LOCUS       DAJVQG010000001        86778 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJVQG010000001 DAJVQG010000000
VERSION     DAJVQG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 86778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86778
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.001"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(50..986)
                     /gene="ldtA"
                     /locus_tag="OQM83_000001"
                     /pseudo
     CDS             complement(50..986)
                     /gene="ldtA"
                     /locus_tag="OQM83_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
     gene            complement(1048..2127)
                     /gene="cobT"
                     /locus_tag="OQM83_000002"
     CDS             complement(1048..2127)
                     /gene="cobT"
                     /locus_tag="OQM83_000002"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCS1518377.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(2139..2882)
                     /gene="cobS"
                     /locus_tag="OQM83_000003"
     CDS             complement(2139..2882)
                     /gene="cobS"
                     /locus_tag="OQM83_000003"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCS1518378.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(2879..3424)
                     /gene="cobU"
                     /locus_tag="OQM83_000004"
     CDS             complement(2879..3424)
                     /gene="cobU"
                     /locus_tag="OQM83_000004"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCS1518379.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(3558..3995)
                     /locus_tag="OQM83_000005"
     CDS             complement(3558..3995)
                     /locus_tag="OQM83_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HCS1518380.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(4213..4388)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(4477..5153)
                     /locus_tag="OQM83_000006"
                     /pseudo
     CDS             complement(4477..5153)
                     /locus_tag="OQM83_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
     gene            complement(5356..6165)
                     /gene="pduF"
                     /locus_tag="OQM83_000007"
     CDS             complement(5356..6165)
                     /gene="pduF"
                     /locus_tag="OQM83_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HCS1518381.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
     gene            6677..6961
                     /gene="pduA"
                     /locus_tag="OQM83_000008"
     CDS             6677..6961
                     /gene="pduA"
                     /locus_tag="OQM83_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HCS1518382.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            6958..7770
                     /gene="pduB"
                     /locus_tag="OQM83_000009"
     CDS             6958..7770
                     /gene="pduB"
                     /locus_tag="OQM83_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HCS1518383.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQDTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDHPSYI"
     gene            7789..9453
                     /gene="pduC"
                     /locus_tag="OQM83_000010"
     CDS             7789..9453
                     /gene="pduC"
                     /locus_tag="OQM83_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HCS1518384.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTNGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGSFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            9463..10131
                     /locus_tag="OQM83_000011"
     CDS             9463..10131
                     /locus_tag="OQM83_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HCS1518385.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            10146..10664
                     /gene="pduE"
                     /locus_tag="OQM83_000012"
     CDS             10146..10664
                     /gene="pduE"
                     /locus_tag="OQM83_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HCS1518386.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            10674..12506
                     /locus_tag="OQM83_000013"
     CDS             10674..12506
                     /locus_tag="OQM83_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HCS1518387.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            12496..12846
                     /locus_tag="OQM83_000014"
     CDS             12496..12846
                     /locus_tag="OQM83_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HCS1518388.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            12866..13141
                     /gene="pduJ"
                     /locus_tag="OQM83_000015"
     CDS             12866..13141
                     /gene="pduJ"
                     /locus_tag="OQM83_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HCS1518389.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            13165..13587
                     /locus_tag="OQM83_000016"
     CDS             13165..13587
                     /locus_tag="OQM83_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HCS1518390.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            13590..14222
                     /locus_tag="OQM83_000017"
     CDS             13590..14222
                     /locus_tag="OQM83_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HCS1518391.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            14219..14710
                     /gene="pduM"
                     /locus_tag="OQM83_000018"
     CDS             14219..14710
                     /gene="pduM"
                     /locus_tag="OQM83_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HCS1518392.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            14714..14989
                     /gene="pduN"
                     /locus_tag="OQM83_000019"
     CDS             14714..14989
                     /gene="pduN"
                     /locus_tag="OQM83_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HCS1518393.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            15001..16008
                     /gene="pduO"
                     /locus_tag="OQM83_000020"
     CDS             15001..16008
                     /gene="pduO"
                     /locus_tag="OQM83_000020"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HCS1518394.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            16005..17390
                     /gene="pduP"
                     /locus_tag="OQM83_000021"
                     /pseudo
     CDS             16005..17390
                     /gene="pduP"
                     /locus_tag="OQM83_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            17401..18513
                     /locus_tag="OQM83_000022"
     CDS             17401..18513
                     /locus_tag="OQM83_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCS1518395.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLKGLL"
     gene            18510..19855
                     /locus_tag="OQM83_000023"
                     /pseudo
     CDS             18510..19855
                     /locus_tag="OQM83_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
     gene            19858..20412
                     /gene="pduT"
                     /locus_tag="OQM83_000024"
     CDS             19858..20412
                     /gene="pduT"
                     /locus_tag="OQM83_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HCS1518396.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            20412..20762
                     /gene="pduU"
                     /locus_tag="OQM83_000025"
     CDS             20412..20762
                     /gene="pduU"
                     /locus_tag="OQM83_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HCS1518397.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            20767..21210
                     /locus_tag="OQM83_000026"
     CDS             20767..21210
                     /locus_tag="OQM83_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HCS1518398.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(21311..21640)
                     /locus_tag="OQM83_000027"
     CDS             complement(21311..21640)
                     /locus_tag="OQM83_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCS1518399.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(21812..22870)
                     /locus_tag="OQM83_000028"
     CDS             complement(21812..22870)
                     /locus_tag="OQM83_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCS1518400.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(23068..23541)
                     /gene="sbmC"
                     /locus_tag="OQM83_000029"
     CDS             complement(23068..23541)
                     /gene="sbmC"
                     /locus_tag="OQM83_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HCS1518401.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(23660..24826)
                     /gene="dacD"
                     /locus_tag="OQM83_000030"
     CDS             complement(23660..24826)
                     /gene="dacD"
                     /locus_tag="OQM83_000030"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HCS1518402.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVFSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            25035..26462
                     /gene="sbcB"
                     /locus_tag="OQM83_000031"
     CDS             25035..26462
                     /gene="sbcB"
                     /locus_tag="OQM83_000031"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCS1518403.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(26505..26732)
                     /locus_tag="OQM83_000032"
     CDS             complement(26505..26732)
                     /locus_tag="OQM83_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCS1518404.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(26746..27804)
                     /gene="yeeE"
                     /locus_tag="OQM83_000033"
     CDS             complement(26746..27804)
                     /gene="yeeE"
                     /locus_tag="OQM83_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCS1518405.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(27983..29341)
                     /gene="plaP"
                     /locus_tag="OQM83_000034"
     CDS             complement(27983..29341)
                     /gene="plaP"
                     /locus_tag="OQM83_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCS1518406.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(29331..29393)
                     /gene="yoeI"
                     /locus_tag="OQM83_000035"
     CDS             complement(29331..29393)
                     /gene="yoeI"
                     /locus_tag="OQM83_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCS1518407.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(29608..30537)
                     /locus_tag="OQM83_000036"
     CDS             complement(29608..30537)
                     /locus_tag="OQM83_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1518408.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(30583..31407)
                     /locus_tag="OQM83_000037"
     CDS             complement(30583..31407)
                     /locus_tag="OQM83_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1518409.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(31490..31744)
                     /gene="yoeB"
                     /locus_tag="OQM83_000038"
     CDS             complement(31490..31744)
                     /gene="yoeB"
                     /locus_tag="OQM83_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCS1518410.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(31741..31992)
                     /gene="yefM"
                     /locus_tag="OQM83_000039"
     CDS             complement(31741..31992)
                     /gene="yefM"
                     /locus_tag="OQM83_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCS1518411.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            32275..32325
                     /gene="hisL"
                     /locus_tag="OQM83_000040"
     CDS             32275..32325
                     /gene="hisL"
                     /locus_tag="OQM83_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCS1518412.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    32301..32424
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            32471..33370
                     /gene="hisG"
                     /locus_tag="OQM83_000041"
     CDS             32471..33370
                     /gene="hisG"
                     /locus_tag="OQM83_000041"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCS1518413.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            33376..34680
                     /gene="hisD"
                     /locus_tag="OQM83_000042"
     CDS             33376..34680
                     /gene="hisD"
                     /locus_tag="OQM83_000042"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCS1518414.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            34677..35747
                     /gene="hisC"
                     /locus_tag="OQM83_000043"
     CDS             34677..35747
                     /gene="hisC"
                     /locus_tag="OQM83_000043"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCS1518415.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            35747..36814
                     /gene="hisB"
                     /locus_tag="OQM83_000044"
     CDS             35747..36814
                     /gene="hisB"
                     /locus_tag="OQM83_000044"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCS1518416.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            36814..37404
                     /gene="hisH"
                     /locus_tag="OQM83_000045"
     CDS             36814..37404
                     /gene="hisH"
                     /locus_tag="OQM83_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCS1518417.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            37404..38141
                     /gene="hisA"
                     /locus_tag="OQM83_000046"
     CDS             37404..38141
                     /gene="hisA"
                     /locus_tag="OQM83_000046"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCS1518418.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            38123..38899
                     /gene="hisF"
                     /locus_tag="OQM83_000047"
     CDS             38123..38899
                     /gene="hisF"
                     /locus_tag="OQM83_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCS1518419.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            38893..39504
                     /locus_tag="OQM83_000048"
     CDS             38893..39504
                     /locus_tag="OQM83_000048"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCS1518420.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(39601..40578)
                     /gene="wzzB"
                     /locus_tag="OQM83_000049"
     CDS             complement(39601..40578)
                     /gene="wzzB"
                     /locus_tag="OQM83_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCS1518421.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLAQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(40724..41890)
                     /gene="ugd"
                     /locus_tag="OQM83_000050"
     CDS             complement(40724..41890)
                     /gene="ugd"
                     /locus_tag="OQM83_000050"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCS1518422.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(42140..43546)
                     /gene="gndA"
                     /locus_tag="OQM83_000051"
     CDS             complement(42140..43546)
                     /gene="gndA"
                     /locus_tag="OQM83_000051"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCS1518423.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(43710..45080)
                     /gene="cpsG"
                     /locus_tag="OQM83_000052"
                     /gene_synonym="manB"
     CDS             complement(43710..45080)
                     /gene="cpsG"
                     /locus_tag="OQM83_000052"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCS1518424.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPFEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(45185..46621)
                     /gene="cpsB"
                     /locus_tag="OQM83_000053"
     CDS             complement(45185..46621)
                     /gene="cpsB"
                     /locus_tag="OQM83_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCS1518425.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(46624..47847)
                     /gene="wcaI"
                     /locus_tag="OQM83_000054"
     CDS             complement(46624..47847)
                     /gene="wcaI"
                     /locus_tag="OQM83_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCS1518426.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(47844..48323)
                     /gene="gmm"
                     /locus_tag="OQM83_000055"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(47844..48323)
                     /gene="gmm"
                     /locus_tag="OQM83_000055"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCS1518427.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(48326..49291)
                     /gene="fcl"
                     /locus_tag="OQM83_000056"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(48326..49291)
                     /gene="fcl"
                     /locus_tag="OQM83_000056"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCS1518428.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(49294..50415)
                     /gene="gmd"
                     /locus_tag="OQM83_000057"
     CDS             complement(49294..50415)
                     /gene="gmd"
                     /locus_tag="OQM83_000057"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCS1518429.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(50441..50989)
                     /gene="wcaF"
                     /locus_tag="OQM83_000058"
     CDS             complement(50441..50989)
                     /gene="wcaF"
                     /locus_tag="OQM83_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCS1518430.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(51005..51760)
                     /gene="wcaE"
                     /locus_tag="OQM83_000059"
     CDS             complement(51005..51760)
                     /gene="wcaE"
                     /locus_tag="OQM83_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCS1518431.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAHLAQAEDISFEWIVVDG
                     GSNDGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDA
                     ANFVRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGL
                     KKWRYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKV
                     QRQILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(51771..52988)
                     /gene="wcaD"
                     /locus_tag="OQM83_000060"
     CDS             complement(51771..52988)
                     /gene="wcaD"
                     /locus_tag="OQM83_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCS1518432.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(52963..54180)
                     /gene="wcaC"
                     /locus_tag="OQM83_000061"
     CDS             complement(52963..54180)
                     /gene="wcaC"
                     /locus_tag="OQM83_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCS1518433.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(54177..54665)
                     /gene="wcaB"
                     /locus_tag="OQM83_000062"
     CDS             complement(54177..54665)
                     /gene="wcaB"
                     /locus_tag="OQM83_000062"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCS1518434.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(54668..55507)
                     /gene="wcaA"
                     /locus_tag="OQM83_000063"
     CDS             complement(54668..55507)
                     /gene="wcaA"
                     /locus_tag="OQM83_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCS1518435.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(55600..57762)
                     /gene="wzc"
                     /locus_tag="OQM83_000064"
     CDS             complement(55600..57762)
                     /gene="wzc"
                     /locus_tag="OQM83_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCS1518436.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(57765..58208)
                     /gene="wzb"
                     /locus_tag="OQM83_000065"
     CDS             complement(57765..58208)
                     /gene="wzb"
                     /locus_tag="OQM83_000065"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCS1518437.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(58214..59353)
                     /gene="wza"
                     /locus_tag="OQM83_000066"
     CDS             complement(58214..59353)
                     /gene="wza"
                     /locus_tag="OQM83_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCS1518438.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            59662..59811
                     /locus_tag="OQM83_000067"
     CDS             59662..59811
                     /locus_tag="OQM83_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1518439.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            60012..61595
                     /locus_tag="OQM83_000068"
     CDS             60012..61595
                     /locus_tag="OQM83_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCS1518440.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(61869..63722)
                     /gene="asmA"
                     /locus_tag="OQM83_000069"
     CDS             complement(61869..63722)
                     /gene="asmA"
                     /locus_tag="OQM83_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCS1518441.1"
                     /translation="MRRFLTTLMILLVVLVAGLSVLVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(63744..64325)
                     /gene="dcd"
                     /locus_tag="OQM83_000070"
     CDS             complement(63744..64325)
                     /gene="dcd"
                     /locus_tag="OQM83_000070"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCS1518442.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(64417..65058)
                     /gene="udk"
                     /locus_tag="OQM83_000071"
     CDS             complement(64417..65058)
                     /gene="udk"
                     /locus_tag="OQM83_000071"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCS1518443.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            65376..68693
                     /locus_tag="OQM83_000072"
     CDS             65376..68693
                     /locus_tag="OQM83_000072"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCS1518444.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWERELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(68803..69651)
                     /gene="alkA"
                     /locus_tag="OQM83_000073"
     CDS             complement(68803..69651)
                     /gene="alkA"
                     /locus_tag="OQM83_000073"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCS1518445.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            69785..71137
                     /gene="yegD"
                     /locus_tag="OQM83_000074"
     CDS             69785..71137
                     /gene="yegD"
                     /locus_tag="OQM83_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS1518446.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(71150..73090)
                     /gene="yegI"
                     /locus_tag="OQM83_000075"
     CDS             complement(71150..73090)
                     /gene="yegI"
                     /locus_tag="OQM83_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HCS1518447.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(73087..73848)
                     /gene="pphC"
                     /locus_tag="OQM83_000076"
     CDS             complement(73087..73848)
                     /gene="pphC"
                     /locus_tag="OQM83_000076"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HCS1518448.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(73845..74504)
                     /locus_tag="OQM83_000077"
     CDS             complement(73845..74504)
                     /locus_tag="OQM83_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCS1518449.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(74725..74784)
                     /gene="ibsA"
                     /locus_tag="OQM83_000078"
     CDS             complement(74725..74784)
                     /gene="ibsA"
                     /locus_tag="OQM83_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HCS1518450.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(75057..75113)
                     /locus_tag="OQM83_000079"
     CDS             complement(75057..75113)
                     /locus_tag="OQM83_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1518451.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(75384..75440)
                     /locus_tag="OQM83_000080"
     CDS             complement(75384..75440)
                     /locus_tag="OQM83_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1518452.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            75719..76960
                     /gene="mdtA"
                     /locus_tag="OQM83_000081"
     CDS             75719..76960
                     /gene="mdtA"
                     /locus_tag="OQM83_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCS1518453.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKTLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            76960..80082
                     /gene="mdtB"
                     /locus_tag="OQM83_000082"
     CDS             76960..80082
                     /gene="mdtB"
                     /locus_tag="OQM83_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCS1518454.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            80083..83160
                     /gene="mdtC"
                     /locus_tag="OQM83_000083"
     CDS             80083..83160
                     /gene="mdtC"
                     /locus_tag="OQM83_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCS1518455.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHLLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            83161..84576
                     /locus_tag="OQM83_000084"
     CDS             83161..84576
                     /locus_tag="OQM83_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1518456.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            84573..85976
                     /gene="baeS"
                     /locus_tag="OQM83_000085"
     CDS             84573..85976
                     /gene="baeS"
                     /locus_tag="OQM83_000085"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCS1518457.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            85973..86695
                     /gene="baeR"
                     /locus_tag="OQM83_000086"
     CDS             85973..86695
                     /gene="baeR"
                     /locus_tag="OQM83_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCS1518458.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
BASE COUNT        21212 a        22993 c        21861 g        20712 t
ORIGIN      
        1 aaaaagcctg ctttctagca ggctttttgc tttctaatta ccaacgctct taaaacatct
       61 gtcttgaacc agaactaatt tgcacaggca ttcccgatcg acgttgcaac gcagcatttg
      121 cgcgatttac atcaacttct tgcccgctga taaacgcccg caaagatggg gttaccggca
      181 atggcacttt tcggtcagac tcatattctg cacgattgcg cgacaacggc tcatgaactt
      241 ccagccagtt cgagccatct ggttcagtgg tgtattttac tggctggtcg ataatttgca
      301 cacgcgtccc aacaggaaca ttatcaaaca gatatttgat atcgtcattg cgcagacgaa
      361 tacagccctg acttacccgg agcccaatac caaaattggc attggtacca tggatggcat
      421 acaacctgcc aatataaatc gcgtacagcc ccatgggatt atcggggccc gcaggaacaa
      481 atgcgggcaa actctcccct cgtttcgcat attcgcgccg agtgttcggc gttggcgtcc
      541 aggttggagc ttcttgttta cgttcaacgg tagtcaccca gttacgcggg gtttctcgcc
      601 cagcctggcc gataccaata ggaaagactt ccacagtatt actgtctggt gggtagtaat
      661 aaagacgcat ctcagcgacg ttaaccacaa tccctttacg aacagtggcg ggcaaaatca
      721 gttgctgcgg aatggtgagt tgcgagccag acttcggcaa aaaaaacatc agcgcccggg
      781 ttcgcttcca gcatgttact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc
      841 tgggtattgt gatcaggaac agttacagta agcgactgcc ccactaaacg gctaccctct
      901 ggaggtaatg gataagttac cgccaggcta ttatggctgg caaaaagcag agcaaatgag
      961 caaaaaatat ttacacgacg catcatgtcc ctttcctaag tcgcgaaagc tatccgttaa
     1021 gtatagcttt tatcagactt ttcgttttta actgttcaaa tcagaagtcg tattccccgg
     1081 tagaacaata ttactggcag caagttcgcc catgttgtta tatatcgcac aggcagcttc
     1141 gatgatgggc atcgccagag ctgcgccact accctcacct aaacgcatct ccatattgag
     1201 ataaggctcc agccccaaat gcgacagcgc aatacgcgcc cctttttctg ccgacaggtg
     1261 agaaggaatg agatacggtt tgattgcagg agacatctgg caggctgcga gcgcggcagc
     1321 ataagaaaga aatccatcca gcaacacggg taaaccgcag gaagcagcgc ctaacatcac
     1381 gccagccatt ccgaccaaat caaatccacc cacttttgcc aggacatcaa cgccatcctg
     1441 cggatttggt tgattcaacg taatcgcccg gcgcacaaca tcaattttat tagccagttt
     1501 atctgtcggc aggtttgcgc caatcccaac cacttcttca ggagcccggc cagtgattgt
     1561 gctgactatt gccgctgccg gtgtcgtgtt tgccatcccc aggtcaccta caccaaacag
     1621 cgtaacaccg ttttttgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc
     1681 tgccagatgg cgactcattg ccggagctga agcaatattg ccactacctc gagcaacacg
     1741 catgttgata agcccgggga taggctcagc agtatcaata ccaacatcaa ttacgtagac
     1801 gttagcgccc gcttgtgctg ccagcacaca cacgccggtt gttccgcggg tcatattttc
     1861 agcctgaatg gctgtcactt cttttgggga aatagcgacc ccttcctccc agacgccgtg
     1921 atcggcacac atgaccagta ccgctttttt gcccacatgc ggaacgccat tcaaccccgg
     1981 cattcctgcc agttgtatgg caagcgcctc cagccttccc aggctaccaa caggtttgag
     2041 taacccgtcg atatgccgtt gtgcacgcga catagcggca ggatcaatag cagggatcgt
     2101 attcagtaaa tcggcaagtg tttgcatgtc acgtctcttt atagcagtgc cagcaggaag
     2161 accagttcac caagctctat agcagctccc agcgtatcgc cggtttgccc gcccagcgta
     2221 cgtttgagaa gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca
     2281 ccatgcatac ccggcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag
     2341 gtttgtcgcc catcaatttt gccaatgaac acattgccaa gtccttcttc ccgggcataa
     2401 cgatgacgat acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc
     2461 gatgcaagga tcggttcgcc acgcagagcc aactcgctta ataccagaat ctttgccagt
     2521 acaacaaaaa tcaatgccag accgccgtgg gtgcctaaac ggctatcacg catgatttcc
     2581 aacatgcgat cgcggctacg tgcagaaaat acgccatcac aggtatcggc gaggccgtcc
     2641 aggtggaatc ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt
     2701 acgccacacc atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat
     2761 ccaattaaag gaaaagtaat aataccgcga gaataatgct cgaaatccag tccctgggac
     2821 cagcgacgcg gtacaggcag acgcgtaata aaagagagca tcgcccaaaa taatttactc
     2881 atttgatttt tactccaata cccgaaacca ccaaccatac ttcatttgcc gcagcggcca
     2941 gctgctgatt tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca
     3001 caatccccat tcccacttcg ttagtcacta atacaacctt tgcgggacaa cgttggcagg
     3061 cagcaatcaa cgactgaatc tcagcattaa tcgcctgttc catcgcctga taatcccatt
     3121 catcagggtc tttatcgccg ccataatcaa acaacagatt agtcaccatg gtggtaacgc
     3181 attccagcaa tacagcctca tgcgggttaa tgtctgcatg aattaattca tcaagatgtt
     3241 gccagcgctc cactgtgtgc cagtgcgccg ggcggccttg ccgatgatgt tctatccgtg
     3301 cagccatctc atcatcaagg atttgtgagg tggcgatata cagaacctgc gaagagtccc
     3361 caataagcgc ctctgcatgg cgactcttcc cgctccgtgc gccccccgtc accaaaatca
     3421 tcataccgac tcctgattca attgtatgat ggtattcatt gaacagaaca atggatcccc
     3481 caccgttgct gaccgcaaaa catttacgcg attaatactg cgcgtaatat aattaaaaat
     3541 ccagcattct caatccatta cgaaagaaga tggcattgcg gaatagccag acttcctcgt
     3601 gttaacgtcc gacaaaatgc gtcattgaca gcggtttctc tggaatgaca gatttcattc
     3661 agataatgga ttaatgctac agccccacca gccggaccag tcagcaaacg ggcattgtgt
     3721 tctgcaatca aaattaccgt cgttccctca gaatgagcga gccattctgg tacggatatc
     3781 tcgctttcag ctgctatcaa tagcagcact gaataatcgc tatacgcatc tgataacgat
     3841 tgcacaggcc caccatcaag aggctggaaa tcttgctgcc agtcctcacc aacaaaacag
     3901 gttattggca atatttcagc cagatgtttc gccagatcca catctccaga taagaggcaa
     3961 aaaagagcta tcgattcggg ttttatcgtt ttcatgcctc tcgccctcca cttaatttcc
     4021 tgtaatggcg gtctggttga taagccatga aacaatattg ccacaccttg aatgcagccc
     4081 tggtgcctgt cggcactgcg atgaaagaac taaatgatgc cgccataccc tgatcctttc
     4141 agggtggtag gggtataaag acgcgccagt actctaccgg acgcttccgg gcaacccact
     4201 tcccaccgaa gttgttggta ttgactgaca gactggtctt ctggcttagc gtcatcctca
     4261 cccgaccttc ccaatctcgc gatcagtggc attttcgggt ttgtcagcat tacagcagcg
     4321 ggggctgcga gggattttca ccctcttccc aggcacaaat gtgccacacc tgtcagcgta
     4381 tgttatgagc tgatttcact tttcccataa cacgtaaata acgttaatta ttcattccag
     4441 cgtttattta aaccttccct ttttttagca tttattctat accgtttcca tcgccgcatt
     4501 ttcactcact tgctgtcgat acacctgcgg cgtaacctga taagtctgac ggaaaacttt
     4561 gcaaaaataa ctgtattgtc gtggggatta ggcgcacgag tctggcgcac cgaatctgac
     4621 cgagatttgt tctctttaat aaccacaaaa ttgagttgtt ttttgaggca attttccaca
     4681 atcaacttca acaaatcagc agaggccata actcttgagt aatccatctc aggaactttg
     4741 cggaattcat taagtaattc gggatctgcc tgccagcaat catcgacatt cagaatatcg
     4801 accaaatcga catcatcatt acgcagacga acttgcccgc acaatacaaa accgactaaa
     4861 tgtccggcga ttaccagtgg aatagagaaa tcggttaatc cggcatgaca gcgataaata
     4921 caaggctgat cggatttaga ggcttccagg cccccacaac gatcgctcat gcgacatcga
     4981 gtgcttagct gagggtgttg ccgcatcatc tgacaaaatg gagtgaagtt aaacagctcg
     5041 gaaatttcat cgccgtgaat attgaccacg acaaccgcaa ggctggtcgc ctgcgcaaaa
     5101 tcctgcgcga ttttattaat gagttctgaa ttaagtgtcg ccgcagaaat catgataaaa
     5161 cctcccagtt aatttattgt tatagcaaaa caattgtttt acatttaatc ttcacatcat
     5221 ccctgatgat ttactgacga gaagcacaaa ggacaataac aaaagcctcc gtgaagcggg
     5281 agacttttta ataagttcgg aaagcagatc acaaacagta aaaaaataaa tgtttataac
     5341 tgccagagta tttcgttatt cgctaattcc tttaggtgta acactattga caggtaaatt
     5401 ctttgcaatg agatagcgat aaatcgccgc accaacacat gccccaatga tcggtgcaat
     5461 aatgggtaca atgaaataag gaatattgcg tccaccagtc atcgcaattt ctccccaccc
     5521 cgcaagccag gtgaaaatct taggtccaaa atcacgcgct ggattcatgg caaaaccggt
     5581 taatggtccg gtagaagcac caataacagc aaccagaata ccaataagta aaggtgcaag
     5641 cggtcctttg ggtacgccat taccgtcatc ggtcaacgcc ataatcaacc ccattaacat
     5701 ggacgttatg accacttcga ccaacgcagc ttgccataca ttaatcgccg ctgccggata
     5761 agtactgaag atactggcga gttgtaaact ttctgcgcta ccacgcacca tattgtgagc
     5821 agattcaaaa tcggtaaaca gactactgta tagagaatat gccagaactg caccaccaaa
     5881 ggcacccgct aattgcgcca gacaaaatgg cagcacttta cgggctggga aagaggcaaa
     5941 cagccacaga gcaatggtaa ctgccgggtt gagatgagcg ccggaaatac ctgccgtaag
     6001 gtaaacagca agcgaaatcc ctaaccccca aatgatacaa atttcccaca gtcccagact
     6061 tgccccggca actttcaggg cgcacaaaca accaatgcca aaaaagagga acagtccggt
     6121 gcccaaaaac tcggcaacgc attgcgcctt gagtgaatca ttcatagtga cacctttttt
     6181 acggagtttt tctttatcag ccaactatgc gctgactgtt gtatataccg agcacaacta
     6241 taaaaaattg ctctgcagaa aatattgcca acatgcatga cagactcgtg taaaaatcgt
     6301 gctaacataa caaattattg tcattaccga atcatgcaac acagcgttag tcagtgttga
     6361 aagtaataat tcccaccaaa cttttacaat aaaaaattgc catcatgaca taataaaatt
     6421 gaaacaaata tcacagttcc ttcttcgaac taaactcaac caaatattac tcgcgattaa
     6481 actcactagc caacgcaatt aattcacatt gaaatatcca caaaacaacc aataaattaa
     6541 caacatatag ttcataaaag caattttata atatttgttc tctattaatt tctctcatta
     6601 gccgattaag tcagccattc tttttcctcg ctttttttta tagtcatatc gtcaaaaaaa
     6661 tccaggcgag gtctttatgc aacaagaagc gttaggaatg gtagaaacca aaggcttaac
     6721 tgccgccata gaggccgcag acgccatggt gaagtcggca aatgttttgc tggtcggcta
     6781 cgaaaaaata ggttccgggc tggtgaccgt catcgttcgt ggtgatgtcg gggcggtaaa
     6841 agcggcaaca gacgcgggtg ccgcagctgc gcataacgtc ggcgaagtaa aagccgtaca
     6901 cgttattccc cgcccacata ccgatgtcga aaaaatctta ccaaagggaa ttaacccatg
     6961 agcagcaatg aactggtcga acagatcatg gcgcaggtca tcgctcgcgt agcgacgcca
     7021 gagcatgagg ttctcccgaa aaccactcat caaatacgag agacggctat ggcagaaaaa
     7081 agctgcacgc taacagaatt tgttggcacc gctattggcg acactgtagg tctggtcatt
     7141 gcgaacgttg atagcgccct gttggacgcg atgaaacttg aaaagcgcta tcgttccatt
     7201 ggcattctgg gtgcgcgtat tggcgcaggg ccacacatta tggccgctga tgaagcggta
     7261 aaagccacca acactgaagt tattagcatt gaacttcctc gcgatactaa aggcggtgcc
     7321 ggacacggct cactgatcct cttaggtggc aacgatgttt ccgacgtgaa acgcggtatt
     7381 gaagtcgccc tgaaagaact cgatcggacc tttggcgatg tctatgccaa cgaagccgga
     7441 catcttgaac tgcaatatac cgcccgcgcc agctatgcac tggaaaaagc gtttggtgca
     7501 ccactgggac gggcttgcgg cgttattgtc ggcgctccgg ctgctgtggg tgttctgatg
     7561 gccgacaccg cgctgaagtc cgccaatgtg gatgttgtgg catacagctc cccagctcaa
     7621 gacaccagct acagcaatga agtcattctg attatctcgg gtgactccgg cgcagtccgc
     7681 caggcggtca tctccgcacg cgaaatcggt aaaaccgtgc tttcgactct cggcgataca
     7741 ccgaaaaacg atcacccttc ctacatctga attctctgag gcagatttat gagatcgaaa
     7801 agatttgaag cactggcaaa acgcccggtc aaccaggacg gtttcgttaa agagtggatt
     7861 gaagaaggct ttattgcgat ggaaagcccg aacgatccta aaccgtcaat caaaattgtt
     7921 aacggcgtcg ttaccgaact ggatggaaaa cccgccagcc agtttgacct gatcgaccac
     7981 tttatcgccc gctacggcat caatctcgcg cgcgccgaag aagtcatcgc gatggattcg
     8041 gtaaaacttg ccaatatgtt gtgcgatcca aatgtcaaac gtagcgatat cgttcccctt
     8101 accacagcaa tgacgcctgc aaaaatcgtc gaagtggttt cgcagatgaa cgtggtagag
     8161 atgatgatgg cgatgcagaa aatgcgcgcc cgtcgaacac catcacaaca ggctcacgtc
     8221 accaacgtca aagataaccc agtacaaatt gccgccgacg cagctgaagg cgcatggcgc
     8281 gggtttgatg aacaggaaac caccgttgcc gttgctcgct atgcaccgtt caacgccatc
     8341 gccctgctgg tcggctcaca ggtgggacgt ccgggcgtct tgacccagtg ttcactggaa
     8401 gaagccaccg aactgaaact cggtatgtta ggccacacct gttacgccga aacaatttct
     8461 gtctatggta cagaacctgt tttcaccaat ggtgatgata ccccgtggtc aaaagggttt
     8521 ctcgcgtcat cctatgcttc tcgtggctta aaaatgcgct ttacttccgg ctccggctct
     8581 gaagtacaga tgggttacgc ggaaggcaaa tcaatgcttt acctcgaggc tcgttgcatt
     8641 tacatcacca aagccgcagg cgttcagggt ctgcagaatg gttcggtaag ctgcatcggt
     8701 gttccatcag ctgtgccatc aggcatccgc gcagtattag cggaaaactt aatttgctca
     8761 tcgctggatc tggaatgcgc ctccagtaat gaccagacct ttacccactc ggatatgcgc
     8821 cgtaccgcac gtttcctgat gcaatttctg ccaggcaccg actttatctc ttccggtttt
     8881 tccgctgtgc cgaactacga caacatgttt gctggttcaa acgaagatgc tgaagacttc
     8941 gatgattaca acgtcattca gcgtgacctg aaggttgatg gtgggctgcg accagtacgc
     9001 gaagaagatg ttatcgctat ccgcaataaa gccgctcgcg ctttacaggc tgtctttgcc
     9061 ggtatgggac taccgcctat taccgatgaa gaagtcgaag cggcaaccta tgcccacggt
     9121 tcaaaagata tgcctgagcg caatattgtg gaagacatca agttcgccca ggagattatc
     9181 aataaaaacc ggaacagcct ggaagtggtg aaagccttgg cgcaaggcag ttttaccgat
     9241 gtcgcccagg acatgctcaa catgcaaaaa gcaaaactga ccggcgatta tctccatacc
     9301 tcagccatca tcgtcgatga cggacaagtg ctctctgcgg tcaatgacgt caatgattac
     9361 gccggaccgg ctacaggtta ccgcctgcaa ggggaacgct gggaagagat caaaaatatt
     9421 cccggtgcac ttgatcccaa cgaaattgac taagggtgag aaatggaaat taacgaaaaa
     9481 ttactgcgtc agattatcga agacgtgctg gcagaaatgc aacccagcga caagtccgtt
     9541 tcatttcgcg cacctgtctc tgcaactgtc ccttccgcgc cagataccgg aaattttctg
     9601 accgaaattg gcgaagccca acaaggcact cagcaagatg aagtcattat tgctgtaggc
     9661 ccggcgttcg gcctggcgca aaccgtcaat atcatcggta ttccgcataa aaacatcttg
     9721 cgcgaagtga ttgccggtat cgaagaagaa ggcattaaag cccgtgtaat tcgctgcttt
     9781 aaatcgtccg atgtcgcatt cgtagcagtc gaaggcgatc gtcttagtgg ttcaggtatt
     9841 gctatcggta ttcagtcgaa aggcacaacg gttatccacc agcagggact tcctcccttg
     9901 tctaacctgg aacttttccc acaagcgcct ttgttgacgc tggatactta tcgtcagatt
     9961 ggcaaaaacg ccgcgcgtta tgcgaagcgc gagtctccac aaccggtgcc gaccctcaac
    10021 gatcaaatgg cgcggccgaa ataccaggcc aaatctgcca ttttgcatat taaagagacg
    10081 aagtacgtgg tcactggcaa aaaaccgcaa gaactgcgcg tgacgtttta acaaaggaaa
    10141 ccttcatgaa taccgatgca attgagtcga tggtcaggga cgtgttgaac cgcatgaaca
    10201 gtttacagga tgcagctcct gtttctgcgg tccccaacgc atcaatactg agcgccaaag
    10261 tgacggatta cccgctggca aataaacatc cggaatgggt taaaacagcg accaataaaa
    10321 cgctcgacga tttcactctg gaaaatgtgc taagcgataa cgtcaccgcc ctggatatgc
    10381 gcattacccc tgaaacattg cgtattcagg ctgcgattgc cagagatgct ggtcgcgatc
    10441 gtctggcgat gaattttgaa cgcgccgctg agcttacctc agtaccagat gatcgcatcc
    10501 tcgaaatcta caatgcgtta cgcccttatc gttcaactaa gcaagaactt attgccattg
    10561 ctgacgatct tgaacagcgt tatcaggcca aaatttgtgc tgcatttgtc cgtgaagctg
    10621 cggaacttta tgtcgagcgc aaaaaactga aaggcgacga ttaaggacga actatgcgat
    10681 atatagcagg cattgatatc ggcaactcat caacagaggt tgcgctggca acgctcagtg
    10741 caactggaga gttgtcgttc gtgagtagcg ccctggagga aaccaccgga atcaaaggta
    10801 cgttgcgtaa cgtccatggt attcaggagg cgctcgcaca ggcaacgaaa aaagtcggca
    10861 tcaatgttag cgatatttcg cttattcgta tcaacgaagc aactccggtc attggtgatg
    10921 tggcgatgga aaccatcact gaaactatca tcaccgaatc aaccatgatc ggtcacaacc
    10981 cgaaaactcc gggcggagtg gggctgggcg tagggttcac catcactccg caagagttat
    11041 taacacgccc ggcggacaca ccttacatcc tggtggtgtc atcggcattt gattttgccg
    11101 atatcgcgac gatgataaac gcctccgtac gcgccggata tcagctgact ggcgttattc
    11161 tgcaacgcga cgatggcgta ctggtcaata accgcctgga aattccttta cctattgtgg
    11221 atgaagtcct ctacatcgat cgtattccgc tcggcatgct ggcagcaatt gaagttgccg
    11281 ttcccgggaa agttatcgaa accctgtcta acccgtatgg gattgccacc gtattcgccc
    11341 tcaatgctga agagactaaa aatattgttc ctgtcgcgcg ggcgctaatc ggcaaccgtt
    11401 cagcagtggt agtgaaaacg ccctctggcg acgttaaagc ccgttcgata cccgccggaa
    11461 atatcgaact gctttctgcc gggcgcacaa cgcgcgttga tgtagctgct ggtgccgatg
    11521 ccattatgaa agcggtcggt gagtgcccaa aactggaaaa cgtgacggga gaaccaggca
    11581 ccaacatcgg cggaatgctg gaacatgttc gccagaccat ggcggaactg accaacaaac
    11641 ccagcaacga aatttttatt caggatttgc tggcgataga tacctctgtc cccgtcagcg
    11701 taaccggtgg tctggcaggt gaattttcac tggaacaggc tgtgggtatt gcctctatgg
    11761 tgaaatccga ccgtctgcaa atggcgatga tcgccagtga aattaagcaa aaactgcatg
    11821 ttgatgttca ggtcggaggc gcagaagcag aagccgccat tcagggggcc ttgactaccc
    11881 ctggaaccac acgtccactg gcaattctgg acttaggtgc aggctctacc gatgcctcca
    11941 tcatcaatca gagtggggaa atcgttgcca ctcaccttgc gggagcgggc gacatggtaa
    12001 cgatgattat cgctcgtgag ctgggcttaa atgatcgtta tctggcagaa gaaatcaaaa
    12061 agtacccgtt agcaaaagta gaaagcctgt tccacttacg ccatgaagac ggtagcgttc
    12121 agttcttccc tactccccta tccccccatg tatttgcccg cgtctgtgta gtgaaacccg
    12181 acgaactggt gcctattccc ggtgatttga cgctggaaaa agtacgcgct gttcgtcgca
    12241 gtgcgaaaga gcgtgtcttc gtaaccaatg ccctgcgcgc gctacgtcag gtaagccctg
    12301 ccggaaatat tcgcgacatt ccgttcgtcg ttctggtggg tggttcttcg ctggatttcg
    12361 aagtaccgca attggtcacc gacgcgctgg cacattatcg actggtcgcc gggcggggga
    12421 atatccgtgg tagcgaaggt ccgcgcaacg cggtagctac tgggctactc atcgcctggc
    12481 acaaggagtc gattcatgga aagtaaagag cgtgcaccag ccatcgttgt catggagatc
    12541 ggtgactgta tcactacatg ggatgaggtg cttcttggca ttgaagagga aggtattccc
    12601 tttcgcattc aacacattcc gtcaggtgaa gttatcgata gcgcctggct ggctgctcgt
    12661 caatctcccc tgttggttgg cattgcctgc gaccgggaag aactgattgt gcattacaaa
    12721 aacttaccgg catcagcgcc actgtttacg ctgacgtatc aacaagataa ccatacccgg
    12781 cgcagcatcg gcactaacgc tgcccgcctg gtaaaaggaa ttcccttccg ggaattccac
    12841 tcataatcca caggagaaac gctgtatgaa caacgcacta ggactggttg aaacaaaagg
    12901 gttagttggc gcaattgaag ctgcagatgc catggttaaa tccgccaacg tgcagctggt
    12961 tggctatgaa aagattggtt cgggtctgat tacggtgatg gtccgcggtg atgttggcgc
    13021 agtaaaggcc gccgttgatg cggggagcgc tgcggcaagc gccgttggcg aagtgaaatc
    13081 ctgccatgtc atcccgcgcc cacacagtga tgttgaggct attttaccta aatcggcata
    13141 accccatttg ctaaggagca cagcgtgaag caatcactgg gattacttga agccagtggt
    13201 ctggctctcg ccatcagttg cgcagacgct atggcaaaag ccgcgtccat cacgctggtt
    13261 ggccttgaga agaccaatgg ctcaggctgg acgctcatca aaatcaccgg tgacgtcgcc
    13321 tcggttaatg cggcgatcgc gacgggagcc agtttcgccg gacagcgaaa tggccttgtc
    13381 gcacacaaag tgattgcccg cccgggagac ggcattctgc cccacacaac tgaagcattg
    13441 accgtgacgg ttgaaacaag caaaacacca gcaacaaccg tcacctcaga gattatcagt
    13501 tgtaatttgt gtcttgaccc ggcatgtcag cggcaaaaag gtgagccccg ctcactttgc
    13561 ctgtacgcca ataagcgagg ggagtagcag tggataaaca aaccttgcag gaaacagtga
    13621 gccagattct ggatgagatg cgcgagcgcc ctatcccact gggcgtttcc agtcgccata
    13681 tacatctttc atcagcggat tatttgcgtc tttttcctga acaaccgatt agcgagaaaa
    13741 agccgttaat gcaacctggc caatacgctg ctgaacaggt tgttacgctg gagggaccaa
    13801 aaggcaaatt gcacaacgtt cgcttgttgg ggccgctacg caatgcaagc caggtggaaa
    13861 tttcccggac ggacgccaga acgttaggga tcaccgcccc gctacgcatg tcaggcgatc
    13921 tcagcggcac tccggggatt cgtttgataa gcccatttgc ggaactggag atcacatctg
    13981 gcgtcattgt cgcgcaacgg catattcata tgtctccgct ggatgccctg atttatggcg
    14041 ttacgcatgg cgataaagtg gcggttgcca ttgaaggtga tgagcgacga ctgatattcg
    14101 aaaacgtcgc tgttcgcgtc tctccggata tgcgtcttga aatgcatatt gataccgatg
    14161 aggccaacgc cgcaggcgct gatagccctg acgtttttgc cacactggtg tcagtgccat
    14221 gaataacatg ctgatgcaac gtattgttga cgaagtggtt tttcgcctta aacaacgtgc
    14281 gggaaaaaca ctcgtgctga ctgtttttca attgcgagac gccagcgttc aagagagcgt
    14341 tcatcagtat gcgtcattac aaatccgata tgtcgatctg ccgctgctgc gacaacttgc
    14401 ggagaacgaa acgtcagatc gagcatctat ccagatacac gaagcgttag cctggggaat
    14461 gcacattcaa ttgtcgttgc agcgtcattt cctgaacgcc atcgaattga agactctcgc
    14521 ccgtctaccg ctaagctggt gcgacgaaca agggcagccg atctaccttc atcataatcg
    14581 acttttaagt tatgccgata tcgcacagtt aagctcggga atactggtgt tacagcggaa
    14641 atgttgtgtg accgcactgg ctcgtgaagc ggcaattacc cgaaatattc agttaatcag
    14701 gcaggagtaa cctatgcatc tggctcgagt cactggcgcg gtagtatcca cgcaaaaatc
    14761 accttctctg atcgggaaaa aactgctgct ggttcgccgg gtcagtgctg acggcgaact
    14821 gcctctcacg ccattaaccg gcgacgaagt tgccgttgat tcagtcggcg caggcgttgg
    14881 tgagttggta ctcctcagca gtggttccag cgccagacat gtgttttcag gccctaacga
    14941 ggccatcgac ctggcagtgg tcggcattgt cgatacgctt tcgcgctaag gagctaaaag
    15001 atggcaattt atacccgaac aggtgattca ggaagtacgt ctttatttac cggacagcgc
    15061 gtcagcaaaa ctcatctacg tgtcgaaacc tatggcacgc tggatgaact gaacgcgaca
    15121 ctcagtctgt gttattgcgc caccgccatc gaaagccatc gcatcttact ggaagccatt
    15181 caacaacaga tattttggtt tagcgccgaa ctcgccagtg aaagcgaaca gccgtcagcg
    15241 caacagcgtt acatcggtac ggaagaaatt gccgcgttgg aaaacgcgat cgatagtgca
    15301 atgaacgccg tccccccagt tcacagcttc attctgcctg gtcgatgtga agccgcaagc
    15361 cgcatgcatt tcgcccgcac ggtggctcga cgggctgaaa gacggctggt tgaactgaca
    15421 acggaaacca ccgtccggaa tgttttgctg cactatatca accgactttc cgattgcctg
    15481 tatgccctcg cccgcgtgga agacaacgtt gctcatcaaa acctgatgat tcaggaaatc
    15541 acaaaacgtt atcacgaagc caaccacaca ccagcattga aggaacgcac aatgccgctc
    15601 acttttcagg atcttcacca gctcattcgt agtgcggcaa tgcgcgcgga tgaactgcat
    15661 attcccgttg tcatcagcat tgttgatgcc aacggaacag aaagtgttac ctggcggatg
    15721 cctgatgccc tgttggtcag cagtgaactg gcaccgaaaa aagcctggac cgccgtggcg
    15781 atgaaaacgg caacccacaa gcttgctgat accgtgcaac ccgacgcgcc gctttacggg
    15841 ctcgaaagcc atatgcaagg aaaagtcgtc acttttggtg gcggcttccc cctctggcgt
    15901 gacggaaaat tgcttggcgg gcttggcatc agcggcggta gcgttgaaca agacatggat
    15961 attgcccaaa gcgcaatggc ggcaattaac gtgggagtaa accaatgaat aactctgaat
    16021 tagaaacact gatccgcaat attctgcgtg agcaacttgt ccccgctaca ccacgaacgc
    16081 aaagaaacgc tattttccag actgtcgatg aagcggtctg cgcagcacac taggcctttt
    16141 tacgctttca acagtgcccg cttaaaaccc gtagcgccat tatcagcgcc attcgcgaag
    16201 aattaatgcc ccgccttacc gaactggcag aagaaagcgc caaagaaacg gggatgggaa
    16261 ataaagaaga taagtaccta aaaaacaaag cggcactcga taacacgcca ggaattgaag
    16321 atttaaccac cacagcacta accggtgatg gtggcatggt tttgtttgag tactctccgt
    16381 ttggggtgat tggctcggtc acgccaagca ccaaccccac ggaaaccatc attaacaaca
    16441 gcatcagcat gctggcagca ggtaatagcg tctacttcag tccacacccg ggtgccaaaa
    16501 acgtttcgtt aaaactgatt accacaatcg aagacatcgc gtttcgctgt agtggcattc
    16561 ataacttgat cgttaccgta tctgaaccga cctttgctgc caccgaacag atgatggcgc
    16621 accctaaaat cagcgttctg gcgattaccg gcggtccggg gatcgtcgcc atgggaatga
    16681 aaagtggcaa gaaagtgatt ggtgccggag cgggtaaccc tccgtgcatc gtcgatgaaa
    16741 cggcggatat tgtgaaagcc gccgaggaca tcatcaacgg cgcttcattt gattacaacc
    16801 tgccgtgtat tgctgaaaaa agcctgattg tcgtggcatc agtggcagat tatttaattc
    16861 agcagatgca aagttttggc gcacttcttc ttaactatga gcaaactgag aaattgcgtg
    16921 caatttgcct gccagacggt agcgccaaca aaaaactggt tgggaaaagc ccatcagcct
    16981 tgctggaagc ggcaggcctc cctttgcccg caaaagcgcc gcgcctactg attgctgttg
    17041 tcgacgccga tgatagttgg gttacttgtg agcaactaat gccaatgctg cccattgtga
    17101 aggtcaagga ttttgacagc gctctaaccc tggcgctgaa agtggaagac ggcttgcacc
    17161 acacggcaat tatgcactct cagaacgtct cacgactcaa ccttgccgcg cgcgttatgc
    17221 aaacctcaat atttgtcaaa aacggcccct cctgggcggg tattggcgtc ggcggcgaag
    17281 gctttacgac ctttaccatt gcaacaccga ctggagaggg tacaacgtca gcacggactt
    17341 ttgcccgctc acgacgctgc gtgttaacca gcggcttttc aattcgctag agaaacggaa
    17401 atgaatactt tttcactgca gacgcgttta tacagcggac caggaagtct caaggtactt
    17461 agccgtttct ctcatagaca catctggatt atttgcgatg cctttctggc acgctcacca
    17521 cttattgaca cgttgcatca ggcgctgccc gacagtaatc gtctcagtat ttttagcgaa
    17581 ataacgcccg ccccaactat ccagaccgtg gtgaaaggca ttgcgcaaat gcagacttta
    17641 cgcccggatg tagttatcgg ttttggcggc ggttctgccc tggacgccgc aaaagcaatt
    17701 gtctggttcg gtcgccagtg tggcattgaa attgaaacct gcgtagccat tcctaccacc
    17761 agcggtatcg gttccgaagt gaccagtgct tgtgtcatca gcgatcctga gaaagggatt
    17821 aaatatcccc ttttcgataa tgcactttat ccggatatcg cgatccttga tccatctttg
    17881 gtcgtcagcg tgccaccggc gataacggcc aataccggga tggatgtttt aacccatgcg
    17941 cttgaagctt atgtttctcc tcgcgccagc gacttcacgg atgcactggt cgaaaaagcc
    18001 gtgcaaattg tttttcaata cctgcccact gcagtgaaaa aaggcgattg tcttgccacc
    18061 cgtggcaaaa tgcataatgc atcgacgctt gcaggaatgg cctttagcca ggccggactc
    18121 ggtttaaacc atgcaattgc tcatcaactc ggcggacagt ttcatctgcc gcatggactg
    18181 gcgaatgcgc tcctgctcac ggcagttatc cgctttaatg ctggcgatcc gcgcacagcc
    18241 aaacgctatg cccggctggc gaaaacgtgc catttatgcc cggacaacgc caatgacacc
    18301 gccagcctga atgcacttat tcaacatatt gagcagctta atacaacctg tacgctgccg
    18361 actcttgcca atgcgctgaa agaaaaaaaa gcagaatggt caatacgcat accagatatg
    18421 gttcaggcgg cgctggcgga tgcaacgttg cgtaccaacc cgcgtgcagc tgatgcctcc
    18481 gcgattgcag aactgctcaa ggggttgtta tgaacgtcgg attaatctcc tgcgacgacc
    18541 agaccatccg cgagcgagta caagcagcag gtgtcgtggg tgctggcggc gcaggttttc
    18601 caacccatat taagctacaa gcgcgtgctg acactttcct ggtgaatgcg gcggaatgcg
    18661 aacccatgtt gaaagtagat caacagttaa tggcgcagca agccagccgc ctgcttcgcg
    18721 gtgttcacta tgcgatgaaa gccaccgggg ccagtagcgg aatcatcgcg ctgaaagaaa
    18781 aatatcaacg ggcaataaac gcactcacac cactgttacc accggatatc cgcctgcata
    18841 ttttgcccga cgtttacccc gcaggagacg aagtgctgac catctggatg gcaactggtc
    18901 gccgcgttcc acccgctgca ttacccgtta gcgtcggtgt ggtggtcaat aatgttcaaa
    18961 ccgtacttaa tattgccaga gccgtcgagc aacagtatcc agtgacgcgc cgcacactca
    19021 ccgttaatgg tgcagtggcg aagccaataa cggtaaccgt ccccatcggc atgtctttac
    19081 gtgaagtcct ggcgctggcg ggcggcgcaa cagtggatgc tcctggtttt atcaatggcg
    19141 ggccgatgat gggaagtttg atcacatcgc tcgacacacc agtcagcaaa accactggcg
    19201 gcttgctggt tttacccaac tctcattcac tgatccaacg tcgaatgcag aatgaccgca
    19261 gcgtacttgc cgtcgcgaaa actgtttgtg agcaatgccg cttgtgtacc gatctctgcc
    19321 cacgtcattt aatcggtcac gaactggcac cccatttatt agtacgtgcg cggttaacta
    19381 ccaacagctc gccacgccgc aactgttact gacagcgctc acctgttcag agtgcaacgt
    19441 ctgcgccagt gtcgcctgtc cggtaggaat ttcaccaatg cgcataaacc gcctgcttaa
    19501 gcaggaatta cgcgcacaaa atctccgcta cgaaggtgcc ttaaatcctg cagatcccat
    19561 ggcaaattat cgactgattc ccgtaaaacg gctggtaacc agacttggct taaccccgtg
    19621 gtatcaggat gcgccattgt ctgaacaggt gcctcagccc gaaaaagtga cgttattgtt
    19681 acgccagcac atcggtgcca gcgcaattgc gtgtgtacaa aaaggcgatc gcgtcgttca
    19741 tgggcaatgt gtaggtcaaa ttccacacgg cacattaggt gcccccattc atgccagtat
    19801 tgatggcatg gtcagcgatg tgacagaaaa cgcgattacg cttgtgagag gttaaccatg
    19861 tcgaaagcgg taggtatttt agaacttagc agtatcgcga aagggatgga aaccggcgac
    19921 gccatgctga aaagcgccaa cgttgagtta ctggtcagca aaacgctatg ccccggaaaa
    19981 tttctgctga tgctcggtgg cgatgtgggc gcggtgcaac aggccatcaa cagcggagcg
    20041 tcccttgctg gtgaaatgct ggtcgacagt cttgtcctgc cgaatattca tcccagcctc
    20101 ctttctgcaa tcagcggact taaccatgtt gaacagcacc gggctgtcgg cgtagtcgaa
    20161 acctggagtg tcgccgcctg cattgacgct gccgattgtg ctgtcaaagc tgcaaatgtc
    20221 attcttgttc gcgtacatat ggcttttggc attggcggta aatgctacat ggtggttgct
    20281 ggcgatattt cagatgttaa taacgcggtc tctgccgcca gcgtgcgcgc gggggaaaaa
    20341 ggtttgttgg tctatcggtc ggtgattgcc cgtccacatg aagccatgtg gcgtcagatg
    20401 gtggagggat aatgacaact cagcacacaa ctgaacggat gattcaggaa tatgtcccag
    20461 gtaagcaggt cacgctggca catgtcatcg ccaatccagg gaaggatctg tttaagaaat
    20521 tgggtttacc agacaccatt tccgctattg gtattctgac aattaccccc agcgaagcat
    20581 ctatcatcgc atgcgatatc gccacaaaat caggcgctgt agagatcggt tttcttgatc
    20641 gttttaccgg tgccgtagtg ctgactggtg atgtttctgc gatcgaatat gcacttagcc
    20701 aggttacccg tacgctgggt gaaatgatgc gctttaccgc ctgccccatt acgcggacct
    20761 gacgttatgc aacgtatgat gctcatcggc cccagccagt gcggaaaaac gtcactcacg
    20821 caatgtctgc gtggcgaaaa tattcattat cagaaaacgc aggcaatcgt ctggtccccg
    20881 accaccattg atacaccagg cgaatacctc gaaaaccgca gcctctacag tgcactgttg
    20941 gtcagtgcct gcgaagcgga cattatcgcg ctggtgctta atgcggacgc accgtggtca
    21001 cctttttcgc caggctttac ctcgtcaatg aatcgcccgg tcattggagt agtaaccaaa
    21061 gcagatttag ccaacatgga acaaatatct ttggtgaaat gttggttacg ggaagctgga
    21121 gcgcacaacg tgttagtaac cagtgcggtt aataaccatg gagttgcaga acttttagcc
    21181 ttgctgcata cagaagaagg ctgtcgctaa tttcgacagg ttatcagatg ggaatgatga
    21241 cggtgataca cagaaaagaa tcaatgagca atgagggttg ccgggcaacc ctcattgaat
    21301 aaaacaggaa ttacttcgct tcgccgtttt tcatttcgcc catagctttc agctttttgg
    21361 agatatcgcg gcgttctttg gagagctcgg catttttgat gatgtaatcg tcaacgcgat
    21421 cttcatagtc acctttcatg ctggcgatga tgccctggat tgcttcaacg ctcatcccag
    21481 gcttgatgta atcgctcagg ttgtccagca gcaggacgcg cttctggttg tcacggatct
    21541 ttttctcaac gtcctgaatt tcacgttgca gtttgttctt acgacggaac agacgaacaa
    21601 attccagtac gtcctgaaat gaaggcttgg tagtttccat ttttataccc ctgataatgt
    21661 gagagtcgga ttcgtttaag caaccgctat tcgttagggc caacattact catagctgcc
    21721 gcagatgaca atgcttttat cccttttcac tatcataccc tttatccttg ctgaatcgaa
    21781 gcagcagcaa gatgattctg aagttcagga attatttgcg caaggcccgg caaatcagat
    21841 tcgatagcaa ctcaagctga tgcgccgttt ccatgttcag ccacacataa ccatagattg
    21901 gtgtttccac taccttcagg tcatggtggt tattgagcaa ctgacgcagc tcttccacag
    21961 catcgttcaa tttttccgta ttggcaaaaa ccggctgcgg attaccttcg tacagcgcat
    22021 gaacaaagct cagcagtatt tgctgcatca tgtgctgggt atcacgaagt ttttgcgcgt
    22081 tcaataacac gaaatggctg gggcgcgtgg cccagtacgc attgatttgc aactccagca
    22141 tacaaaccag attgcggtta atggtctgga taccttcata tatcgatttt ggaatacggg
    22201 tttctttgct ggcgggcgca atcagcccac gcattttcac ggcatcggtc aggagttttt
    22261 gtagatggct ttccagacgt gggcgttcga gtaagttcgg tgagaatgca gattgataga
    22321 cccgattata ctcggtcaga cttttcgcca gttgaatgcg ccagtggatg aacgcccgtt
    22381 gtggccagat accggtaaac aacattgcca gtaaagagcc gaggatcaca tcgccgcttc
    22441 gccataacgc cgtatcaatt tcacctgtcg gggaaccaac aacaattgcc agcgtcaccc
    22501 caatcaataa tccttgatac ggtttcttgc ccagcgccag ccaaccgcaa agaaacatcg
    22561 ccgccgcgca ccagactaac atcagcggta acgaaattaa ctccagttgc agagcgataa
    22621 gacctaaaat cgaacccaac accgtaccgc caatacgttc gaaggcacga gggacaacgt
    22681 taccccagaa cgagattggc cccataatca ccaccatggt gaccagcggc caggtgcttt
    22741 ccgggatagt aaacaggcgg ataatgagaa aagtgagcag gaatgccagc gcgacccgag
    22801 taccatgcac tatgcggtaa tggcggtata cccggatttc aaacgggctt aatgacttat
    22861 cggcacgcac gcagaaactc cgtaatgaag attaaaagcg ctcataactc atctgtcggc
    22921 cagaaggcgc gcctctgaat tatccgacgg acaaaccggg aacagtgtaa tttcccggca
    22981 ggagcgaaaa aattgaaagg cgcatcttac tcttttcgct ttcaaaaaaa gagtggtcat
    23041 cgcgttaaca caccgccctg agatgaatta gtgatgtttt ggctgcaccg caacatacat
    23101 ttcgatatcc cagtacccat cttccgcgcc attgttcaaa taaacctcga agcagggctt
    23161 tggtaacatt tcataagcac tgtcctgcaa gaggctatta aagaactgat accagggttt
    23221 agcaaaatca tcaccgacta cacgagccac cgccaccgca tactgaccac ctgaaatttc
    23281 tgtcagaatg acgccctcac tgttttcggg aagcgtaaag ttattcggta ccgtcacaac
    23341 ggtgtcgcag cgtaattttt cggcgggtgt ttcatctgga ttgtcgtaat agacagcaac
    23401 ccactccttc ggcacaatat ttttgttatc tacccacatc atcaactgct caaagccttt
    23461 ctttaccgtc tgttcccacg ggccaacgag atggaaacct gcaacggtac gtttctcttc
    23521 ctgcgtaatc tcgtagttca tgacgcctcc attaacactg tttttatata cagcatagtt
    23581 gcaatattaa aaccacaagg aatgagtgtt gattatgcga gcagactcgc actcctgcca
    23641 gtctgctgca aaagaaaggt caggccttat ggtggaaata atcactcagg cgggaaaaca
    23701 tgccgccttc cccgacagat tccagggtaa ccagcggcca gtgcgccacc tgtttatcac
    23761 ggtcgtaaag ttcaatttcc cctacccgct gatgggcgct aattggcgcg gtgagctctt
    23821 taccatcaag ggtatatttg gctttgatat gtggaatttc ggctttcggt agcaccatcc
    23881 agaactcttg ttccgttcct aggtcgatat tttctttatc gccataccag atgcgttccg
    23941 ttccgacctt tttcccacgg tgcaaaattt gcaccgtagt aaagttttgt tgcccccaac
    24001 gcagtaattt tcttgcctct tcctcacgac cttttgcact gtcagccccc attaccactg
    24061 caatgagacg acgctgccca tctacagccg aagcaatgag attaaacccg gcaccagaag
    24121 tatgacccgt tttcaggccg tcaacattca tggttttatc ccacaacaac ccgttacggt
    24181 tttgctgggt gataccgttc caggtgagac ttttctcact gtacatatga taaaactcgg
    24241 gctcgccgtg gatgatagcg cgagaaaaca cagctaaatc ataagccgag ctatgctggc
    24301 caggtgcatc cagaccatgc actgtttcaa aatgcgtatc cttgagatgc agcttctcgg
    24361 catagttgtt catcatttca acaaactgcc gttgcccacc ggcaatatag tcagccagag
    24421 caacacaagc gtcatttccg gaatccacaa ttaaaccacg gcttaaatca cgtaccgata
    24481 cgcgatcgcc ctctttcaaa aacatcagtg aagaaccgac aaacaccgga ttatctttcg
    24541 cccacgcatc gcgccccacg gtgacaatat cgtctggcgt aatgcgatga ctatcgatag
    24601 cgcgatccac gacataaccc gtcatcagct ttgtcaggct ggcgggattg cgctgttgat
    24661 gctcattacc cgcggtgagg atctgaccgg tggtgtaatc catcaatacc caggacccgg
    24721 catgaatctc tggaggctga ggtgaaaaag gaatgttttc cgccgcaaaa ccagacgata
    24781 agttaaaaac gaacaaagaa gcagcaataa taagacggcg tttcaacagc aaaccctcag
    24841 gagtttcaaa tagctgttct ttttacggaa atacttatga actggctgga ataaagtgca
    24901 agaaaatgtg actaccctct catttttatc tgacatgatc tgttgccact cgctgccaaa
    24961 ttgtggcgct aaagctgatt agcacggtga tatttgatac tctggcagac agcagaaata
    25021 acggatttaa cctaatgatg aatgacggta agcaacaatc tacctttttg tttcacgatt
    25081 acgaaacctt tggcacgcac cccgcgttag atcgccctgc acagttcgca gccattcgca
    25141 ccgataacga attcaatgtc atcggcgaac ccgaagtctt ttactgcaag cccgctgatg
    25201 actatttacc ccagccagga gccgtattaa ttaccggtat taccccgcag gaagcacggg
    25261 cgaaaggaga aaacgaagcc gcgtttgccg cccgtattca ctcgcttttt accgtaccga
    25321 agacctgtat tctgggctac aacaatgtgc gtttcgacga cgaagtcaca cgcaacgttt
    25381 tttatcgtaa tttctacgat ccttacgcct ggagctggca gcatgataac tcgcgctggg
    25441 atttgctgga tgttatgcgt gcctgttatg ccctgcgccc ggaaggaata aactggcctg
    25501 aaaatgatga cggtctaccg agctttcgcc ttgagcattt aaccaaagcg aatggaattg
    25561 aacatagcaa cgcccacgat gcgatggctg atgtgtacgc cactattgcg atggcgaaac
    25621 tggtaaaaac gcgtcagcca cgtctgtttg attatctctt tacccatcgt aataaacaca
    25681 aactgatggc gttgattgat gttccgcaga tgaaacccct ggttcacgtt tccggaatgt
    25741 ttggggcatg gcgcggcaat accagctggg tggcaccgct ggcgtggcat ccagaaaatc
    25801 gcaatgccgt aattatggtg gatttggcag gagacatttc gccattactg gaactggata
    25861 gcgacacatt gcgcgagcgt ttatatactg caaaagccga tcttggcgat aacgccgccg
    25921 ttccggttaa gctggtgcat atcaataaat gtccggtgct ggcccaggcg aatacgctac
    25981 gcccggaaga tgccgaccga ctgggaatta atcgtcagca ttgcctcgat aacctgaaaa
    26041 ttctgcgtga aaatccgcaa gtgcgcgaaa aagtggtggc gatattcgcg gaagccgaac
    26101 cgtttacgcc ttcagataac gtggatgcac agctttataa cggctttttc agtgacgcag
    26161 atcgtgcagc aatgaaaatt gtgctggaaa ccgagccgcg taatttaccg gcactggata
    26221 tcacttttgt cgataaacgg attgaaaagc tgttgttcaa ttatcgggca cgcaacttcc
    26281 cggggacgct ggattatgcc gagcagcaac gctggctgga gcaccgccgc caggtcttca
    26341 cgcccgagtt tttacaaggt tatgcagatg aattgcagat gctggcacaa caatatgccg
    26401 ataacaaaga gaaagtggcg ctgttaaaag cgctttggca gtacgcggaa gagatcgtct
    26461 aatttgaagc ctccgccgct ggtacggcgg aggtcaatgt tgagtcaggc tttttgaacg
    26521 gtgatactcc acgctgcatc gccaatctgc tgataatcgg tgatggcatg cccttcttct
    26581 gccgcccact gtgggattgc ttcagttgcc tgggtgcaat caaactcaat taccaactca
    26641 tcgccgctgg ccatttctgc cagtgctgct tttgcttcta tgagtggaaa cggacagacc
    26701 tgcgtcacca catccagctt tttaatcgcc atctttacac cttacttaat ttgccgcagc
    26761 agttgccagt cgcgccttac gctgcggtcg aacatacaca agccaggacg cagtccagac
    26821 tccgagaatc ataaatacaa ggccaatcca gccctgccag gtcatcattg cagtcataac
    26881 cagtccatta ccgataaagc aaccacctgc aatactggca ccgaagccca tcagtacacc
    26941 acctaacccg ctacgtaatg ttgtttgtgc atcagctgcg cgaacgcgga actcacggct
    27001 cgctttggct gcaataaatg accccacgaa gatccctaac accaggaaaa cgccccagtt
    27061 aatgtatttc acgtcacccg cgaccagaaa ttgcagaata ttggccgttg gggaagtgat
    27121 tcctaaccca aacatgcgtc cggttgcttc actcaggggc caggctaaaa gcgcaatcaa
    27181 accgatgagt acagcagtga caaagggatg ccagcgtttt tcaaacagaa tatgagcgat
    27241 cccagttcgg cgcggcggta aggtcgcgac tttgagtttt ggcttcttca actctttcat
    27301 caccacccag agcgttatca ccagcaaaac ggcaaccaac ggccaaacag acaaattaaa
    27361 agtctcagca atagagttat gttcagtact gtagtgctgc aaggtttgat ttaaaccact
    27421 ggcatgtgga gaacgcatca ccgcactcat caccatataa gtgaaaagcg cgatccaact
    27481 gccgatcaat ccctcacccg cgcgatacca ggtcccggtg gcacatccac ccgccagaac
    27541 aattcccagc ccgaagatat acccacctat aacagtacca agccacggga acgcctcggc
    27601 ttcgtaagtc agtaggcccg cctgaattaa cgcaaaaacc cccacgcttt gtaccgaaat
    27661 tgcaatcagc aaggcgtaaa acatgcgatt atttttcgcg atatacatat cgcgaaaacc
    27721 acctgtcagg caaaaacgcc cacgctgcat gacaaatccc agcagagcac cacaaattag
    27781 cccgcttaat atcattgaaa acataaatta attaaccaga tgaatgttaa tgaggaaatt
    27841 attcatgact ggtggaatgc agaccaataa ccaaattctc taaattagaa caaatggtta
    27901 ttaatgagga tttcgataaa aaaaaataaa aaaaccggag ccaaatattc attcgactcc
    27961 ggttgattat tagaaaagat gattatgcaa cgtcttcgta ctgcggaact gggttacgga
    28021 agctcttggt gacgcaagcc aggtaaatca gaccgatagc agcccaaatc agacccagaa
    28081 ccattgagct ttcttccagg ttaacccaca gcgcaccaac ggtcagcgca ccacacatcg
    28141 gcaggaacag atactggaag tgatctttca gcgtcttatt acgcttctca cggatccaga
    28201 actgcgagat gaccgacagg ttaacgaagg tgaacgccac caacgcacca aagttaatca
    28261 gcgctgtagc cattaccagg tcgaagttga ttgccagcag ggcaatcgcg ccaaccagga
    28321 tgatgttcat cgccggagta cgccatttcg ggtgtacata accgaagaag cttttcggga
    28381 atacgccgtc acgacccatt acgtacatca gacgcgctac gcctgcatgc gccgccatac
    28441 cggacgccag tacggtaatg gtggagaaga tcagcgcgcc aacctggaac gctttacccg
    28501 caacgtacag catgatttca ggctgtgacg catccggatc tttaaagcga gagatatccg
    28561 ggaagtacag ctgcaggaag taagttgcaa agatgaagat catgccgcca atcagcgcgg
    28621 tcaggaaaat cgcacgcggg atcacgcgct ctgcatcttt ggtttcttcc gacaggttgc
    28681 tgatgccgtc aaagccggta aaggaaaagc acaggattgt cgccccggta atcatcggaa
    28741 taacatgcgc atcgccagac cagaacggac gagtgctcgc cagcgtacca gcgccttcac
    28801 cttcaaatac gccataaaca accatgccca gaatcaccgc gatcagcact acctgcaaca
    28861 cgacgattac agtgttgaag ttcgctacgg atttcagact acgcaggtta aaggcggtca
    28921 taaaggccac cagcgccacc acaaacatcc acgatgggat ggaaggcacc agagcttcaa
    28981 aatagatttt cgccagcaga atgttgatca tcggcgcgaa cagatagtcg agcagagaag
    29041 accaacccac cataaagccg acagtcgggc taatggattt ctgggcgtaa gtatatgcag
    29101 agccagcaga aggatagcgg cgaaccagct tcccgtagct cagagccgta aacaggatcg
    29161 caatcaatgc gaacgcatag gctgtcggca catgaccatc cgtgaggcct gaaacgatac
    29221 caaaagtatc aaacagcgtc atcggctgca tataggcaag acccatcatt acaaccggaa
    29281 ctaacgtaag cgttttacgc aattccacgc gagaggtgtt tggagtaacg ttatgcgaca
    29341 tggtcattct cctttacggt gataaccgtc gcgtaagcaa aaaattgccc catttttttg
    29401 gattcctcag cgacaacaac tgtcgatttt tagtaaatat ctatccggta cgaagcccgg
    29461 cctcttggta tgaatgattg gtttgaagca aaaaataacc gacgctgatg aaacgtcggt
    29521 ttttagtcat tttttgacag cggcgcattg tgcctaaatg ggggggaaat gacaagagaa
    29581 tgagaagctt gtcataataa tttttcttta aatggctgat tttccgtcat cagattttgc
    29641 ataaacaccg gcgatcgcac tatttgctaa aatttcatcc cgccaccagc cgctggcaag
    29701 ccctgcggtc tgttcgttcc agccaatcca gacgggttca aagagagttt gggcgaccac
    29761 tttcttctcg attaacgcgc cactatcgag aaaacgttgc accagataac ggggtaaata
    29821 gccgcagccg aggccgctaa tttgcagctc cagcttggtt ttaaaatcga aaacggtaat
    29881 ggcttcttgt tcgtcgagaa gttgtgaggc cgtagaagcg cctgcctgcg cagtgtcccc
    29941 caccacaatc gcccgatatc gcttaatgat acgacggttt aacggctctt cttcctgggc
    30001 taacggatga tgaggcgcga ccgcaaagac ttgttccaga tcgcccaacc gcgagaaacc
    30061 aaactcactg gaagatggtg gctcatgcat cgcgccaaca ataatatccg ctcgcccctg
    30121 agtcagggca tcccaggaac caccgagtac gccattgata aacttcaggc gcgtcacgct
    30181 atgatgttga taaaaagctt caatcagtgg cgcaagcaga gaaaaaggaa acgtgtcgtc
    30241 cacgccaatc accagctcgt tttcccagcc ttcatgcagt ttgatcgcct gcttttccag
    30301 ttctcgcacg gtatgcagaa cttcccgccc cttctctaat aacatttttc cggtacgggt
    30361 gaatttagcg cggtgaccgc tacgatcaag taattgaata ttaaggtcgc tttccagctt
    30421 gtgaacggtg taactcagag cggacggcgt cttgtagagt ttagccgacg ccgccgcaaa
    30481 actgccttct ttttctaagg catcgaggat catcaacaca tccagcagtt gtttcataca
    30541 cgcccccttg cagtgcatgc ggcgtcctgc tggcgtgatc gtttactcca gcggcattac
    30601 cagcggatcg ggatactggt attcaaatcc cagttcatta caaatccgac tgccatcaat
    30661 aatcttacct ttgccgctgt ccagactatt tctgaactgc ggaggttcca gccccagtaa
    30721 acgggccatc tgcggataga aaacattacg cgcagggtga gctggcgcac atatattata
    30781 gatgtgtccg cctttaggtg cctgtaacaa cagagtgata gcgccaataa catcttctaa
    30841 atggactaaa ttaacaccat gttcaccatc aggcgcggtt tttccggcaa agaagcgtcc
    30901 gggatgacgt cccggtccga ccagccccgc aagacgcaga atatcgaccg aagtaccggg
    30961 taaattgtgc agccagtctt cgagttcttc taatactcgt ccactgttgg tcaccggatt
    31021 acgcggggtg gtttctttca ccgtgccttg cgcgtcgcca tagacagacg ttgagctggt
    31081 aaaaataatg cgaggaatac gatgggccag cgcgctatcc actaactctt gtaccgcttg
    31141 taaatagaac tcatcgccgg ggccgctacg acgtgccgga agcgtaatga ccagcgcatc
    31201 ggcatccatc agggcatcca gatcgtcaga atcgcaaact aactcaggct ccatgcgaag
    31261 cagatagcta tcaatgccac tcattcgggc cgcttcgaca ccatcttgtg tggttttact
    31321 cccggtgact tgccagcctc gcgctgaaag tgacatcgcc agcggcatgc ctaaccaccc
    31381 taacccgaca attgcgacct ttttcatccg ttatctcctg atacacctta gatctataag
    31441 gctacgctag cgtatcaaaa ctgacaattc attctatgaa tgaatctgtt caataatgat
    31501 aacgacatgc tgcaatgagc agtgaatcgt cggtaaccgc gtataccaga cggtgctcct
    31561 ctgtaatgcg tcgggaccag aaacctgaca aattatgttt caggggttct ggcttcccct
    31621 taccttcaaa tggcgttctg cgggtatctt tgataagttc attgatcttt ttaacaattc
    31681 gcttatctgt ttcctgccag tacagataat catcccatga ttcctcagac cagattagtt
    31741 tcactcaata atgtcctttt ctgttccttt gcctgatttc aggctatcga ttgagtccat
    31801 caatctccgg gcgttagcgg gggagcgcag tagataagcc gtctcttcca gcgagttgta
    31861 ttcttcgagt gacatcagaa cacaagcctc tccattctga cgagtaataa ggatcggggc
    31921 atgatcttca acggctttca tcattgttgc cgacaaattc tgacgcgctt cgctgtagct
    31981 aattgtacgc atgtcaatct cctcttttgt acagtttatt gtacaatgat gggcgttaat
    32041 taactattta ttaattagtt tgtagatcaa ggtattgtca gtgagacgaa aatccaggct
    32101 tcgctatttt tggtgccgtc agcttagagg acagtcctct tagccccctc ctttccccgc
    32161 tcattcatta aacaaatcca ttgccataaa atatataaaa aagcccttgc tttctaacgt
    32221 gaaagtggtt taggttaaaa gacatcagtt gaataaacat tcacagagac ttttatgaca
    32281 cgcgttcaat ttaaacacca ccatcatcac catcatcctg actagtcttt caggcgatgt
    32341 gtgctggaag acattcagat cttccagtgg tgcatgaacg catgagaaag cccccggaag
    32401 atcaccttcc gggggctttt ttattgcgcg gttgataacg gttcagacag gtttaaagag
    32461 gaataacaaa atgacagaca acactcgttt acgcatagct atgcagaaat ccggccgttt
    32521 aagtgatgac tcacgcgaat tactggcgcg ctgtggcatt aaaattaatc ttcacaccca
    32581 gcgcctgatc gcgatggcag aaaacatgcc gattgatatt ctgcgcgtgc gtgacgacga
    32641 cattcccggt ctggtaatgg atggcgtggt agaccttggg attatcggcg aaaacgtgct
    32701 ggaagaagag ctgcttaacc gccgcgccca gggtgaagat ccgcgctact ttaccttgcg
    32761 tcgtctggat ttcggcggct gccgcctttc gctggcaacg ccggttgatg aagcctggga
    32821 cggcccgctc tccttaaacg gtaaacgtat cgccacctct tatcctcacc tgctcaagcg
    32881 ttatctcgac cagaaaggca tctcttttaa atcctgctta ctgaacggtt ctgttgaagt
    32941 cgccccgcgt gccggactgg cggatgcgat ttgcgatctg gtttccaccg gtgccacgct
    33001 ggaagcaaac ggcctgcgcg aagtcgaagt tatctaccgc tcgaaagcct gccttatcca
    33061 gcgcgatggt gaaatggaag aatccaaaca gcagctgatc gacaaactgc tgacccgtat
    33121 tcagggtgtg atccaggcgc gcgaatcaaa atacatcatg atgcacgcac cgaccgaacg
    33181 tctggatgaa gtcatcgccc tgctgccagg tgccgaacgc ccaaccattc tgccgctggc
    33241 gggtgaccaa caacgcgtag cgatgcacat ggtcagtagc gaaaccctgt tctgggaaac
    33301 gatggaaaaa ctgaaagcgc tgggtgccag ttcaattctg gtcctgccga ttgagaagat
    33361 gatggagtga tcgccatgag ctttaacaca atcattgact ggaatagctg tactgcggag
    33421 caacaacgcc agctgttaat gcgcccggcg atctccgctt ctgaaagcat tacccgcact
    33481 gttaacgata ttctcgataa cgtgaaagca cgcggtgatg acgccctgcg ggaatacagc
    33541 gcgaagtttg ataaaaccac ggttaacgcg ctgaaggtgt ctgctgagga gattgccgcc
    33601 gctagcgaac gcctgagcga cgagctaaaa caggcgatgg cggtggcagt aaaaaatatt
    33661 gaaaccttcc acactgcgca aaaactgccg ccggtagatg tagaaacgca gccaggcgtg
    33721 cgttgccagc aggtcacgcg cccggtcgct tcagttgggt tgtatattcc tggcggctcc
    33781 gccccgctct tctcaacggt attaatgctg gcgactccgg cgcgtattgc gggctgtaaa
    33841 aaagtggtgc tgtgctcacc gccgccgatt gccgatgaga tcctttatgc ggcgcagctg
    33901 tgcggtgtga aggacgtgtt taacgtcggc ggcgcacagg ccattgccgc gctggcgttt
    33961 ggtacggaat ctgtgccgaa agtggacaaa atcttcgggc cgggtaacgc ctttgtcacc
    34021 gaagcgaaac gtcaggtgag ccagcgtctg gacggtgcgg ctatcgatat gcccgcaggc
    34081 ccgtcggaag tgttggtcat tgccgacagc ggcgcaacgc cggatttcgt ggcttctgat
    34141 ttgctttctc aggctgaaca cggcccggac tcacaggtga ttttactgac gcctgacgct
    34201 gatatggcgc atcaagttgc cgaagccgtc gaacgccagt tagcagaact gccgcgtgcc
    34261 gaaaccgccc gccaggcact gaacgccagc cgcctgatcg tgactaaaga tttagcgcag
    34321 tgcgtagaga tctccaacca gtacggcccg gagcacctga tcatccagac ccgcaacgcc
    34381 cgcgatctgg tcgatggcat caccagcgcc ggttcggtat ttcttggtga ctggtcaccg
    34441 gaatcggcag gtgattacgc ctccggcacc aaccacgttc tgccgactta cggttacacc
    34501 gccacctgtt ccagcctcgg gctggcagat ttccagaagc gtatgaccgt gcaggaactg
    34561 tcgaaagaag gtttctccgc gctggcttcg accattgaaa cattggccgc cgccgagcgc
    34621 ctgaccgccc ataaaaatgc cgttactttg cgtgttaacg cccttaagga gcaagcatga
    34681 gcaccgtgac tattaccgat ttagcgcgtg aaaacgtccg caacctgacg ccgtatcagt
    34741 cggcgcgtcg tctgggcggt aatggcgacg tctggctgaa cgccaacgaa taccccactg
    34801 ccgtggagtt tcagcttact cagcaaacgc tcaaccgcta cccggaatgt cagccgaaag
    34861 cggtgattga aaattacgcg caatatgcag gcgtaaaacc ggaacaggtg ctggtcagtc
    34921 gtggcgcgga cgaaggtatt gaactgctga ttcgcgcttt ttgcgaaccg ggtaaagacg
    34981 ccatcctcta ctgcccgcca acgtacggca tgtacagcgt cagcgccgaa acgattggcg
    35041 tcgagtgccg cacagtgccg acgcaggaca actggcaact ggacttacag ggcatttccg
    35101 acaagctgga cggcgtaaaa gtggtctatg tttgcagccc caacaacccg acagggcaac
    35161 tgatcaatcc gcaggatttt cgcactctgc tggagctaac ccgcggaaaa gccattgtgg
    35221 ttgccgatga agcctatatc gagttttgcc cgcaggcatc gctggctggc tggctgacgg
    35281 aatatccgca cctggctatt ttgcgcacac tgtcgaaagc ttttgctctg gcgggccttc
    35341 gttgcggatt tacgctggca aacgaagaag tcatcaacct gctgatgaaa gtgatcgccc
    35401 cctacccgct ctcgacgccg gttgccgaca ttgcggccca ggcgttaagc ccgcagggaa
    35461 tcgtcgctat gcgcgaacga gtgacgcaaa ttattgcaga acgcgaatac ctgattgccg
    35521 cactgaaaga gatcccctgc gtagagcagg ttttcgactc cgcaaccaac tacattctgg
    35581 cgcgctttaa agcctccagc gcagtgttta aatctttgtg ggatcagggc attatcttac
    35641 gtgatcagaa taaacaaccc tctttaagcg gctgcctgcg aattaccgtc ggaacccgtg
    35701 aagaaagcca gcgcgtcatt gacgccttac gtgcggagca agtttgatga gtcagaagta
    35761 tctttttatc gatcgcgatg gaaccctgat tagcgaaccg ccgagtgatt ttcaggtgga
    35821 ccgttttgac aaactcgcct ttgaaccagg cgtgatcccg gaactgctga agctgcaaaa
    35881 agcgggctac aaactggtaa tgatcaccaa tcaggatggt ctgggaacac aaagtttccc
    35941 gcaggcggat ttcgatggcc cgcacaacct gatgatgcag atcttcacct cgcaaggcgt
    36001 acagtttgat gaagtgctga tttgtccgca cctgcccgcc gatgagtgcg actgccgtaa
    36061 gccgaaagta aaactggtgg aacgttatct ggctgagcaa gcgatggatc gcgctaacag
    36121 ttatgtgatt ggcgatcgcg cgaccgacat tcaactggcg gaaaacatgg gcattaatgg
    36181 tttacgctac gaccgcgaaa tcctgaactg gccaatgatt ggcgagcagc tcactaaacg
    36241 agaccgttac gctcacgtag tgcgcaacac caaagagaca cagattgacg ttcaggtgtg
    36301 gctggatcgc gaaggtggca gcaagattaa caccggtgtt ggcttctttg atcacatgct
    36361 ggatcagatc gccacccacg gtggtttccg tatggaaatc aacgtcaaag gcgacctcta
    36421 tatcgacgat caccacaccg tcgaagatac cggcctggcg ctgggcgaag cgttaaaaat
    36481 ggctcttggc gacaaacgcg gtatttgccg ctttggtttt gtgctaccga tggacgaatg
    36541 ccttgcccgc tgcgcgctgg atatctctgg tcgcccgcac ctggaataca aagccgagtt
    36601 tacctaccag cgcgtgggcg atctcagcac cgagatgatc gagcacttct tccgttcgct
    36661 ctcatacacc atgggcgtga cgctacacct gaaaaccaaa ggtaaaaacg atcaccaccg
    36721 tgtagagagc ctgttcaaag cctttggtcg caccctgcgc caagccatcc gcgtggaagg
    36781 cgacaccctg ccctcgtcga aaggagtgct gtaatgaacg tggtgatcct tgataccggc
    36841 tgcgccaatc tgaactcggt gaagtcagcc attgcgcgcc acggttatga accaaaagtc
    36901 agccgtgacc cggacgtggt gttgctggcc gataaactat ttttacccgg cgttggcact
    36961 gcgcaagcag cgatggatca ggtgcgtgag cgcgagctgt ttgatctcat caaagcctgt
    37021 acccaaccgg tactgggcat ctgcttaggg atgcaactgc tgggtcggcg cagcgaagag
    37081 agcaacggcg tcgacttgct gggcatcatt gacgaagacg tgccgaaaat gaccgacttt
    37141 ggtctgccgc tgccgcatat gggctggaat cgcgtttacc cgcaggcagg caaccgcctg
    37201 tttcagggga ttgaagacgg cgcgtacttt tactttgttc acagttacgc aatgccggtc
    37261 aatccgtgga ccattgccca atgtaattac ggcgaaccgt tcaccgcggc ggtacaaaaa
    37321 gataacttct acggcgtgca gttccacccg gagcgttctg gtgccgctgg cgctaagttg
    37381 ctgaaaaact tcctggagat gtgatgatta ttccggcatt agatttaatc gacggcaccg
    37441 tggtgcgtct ccatcagggc gattacggca aacaacgcga ttacggtaac gacccgctgc
    37501 cgcgcttaca ggattacgcc gcgcagggtg ccgaagtgct gcatctggtg gatctgaccg
    37561 gggcaaaaga tccggcaaaa cgtcaaatcc cgctgattaa aaccctggtc gcgggcgtta
    37621 acgttccggt gcaggttggc ggcggtgtgc gtagcgaaga agacgtggcg gcgttactgg
    37681 aagcgggcgt tgcgcgcgtg gtggttggct ccaccgcggt gaaatcacca gaaagggtga
    37741 aaggctggtt tgaacgtttc ggtgccgatg ccttagtgct ggcgctggat gtccgtattg
    37801 acgagcaagg caacaagcag gtggcggtca gcggctggca agagaactcg ggcgtttcac
    37861 tggaacaact ggtggaaacc tatctgcctg tcggcctgaa acatgtgctg tgtaccgata
    37921 tctcgcgcga cggcacgctg gcaggctcta acgtctcttt atatgaagaa gtgtgcgcca
    37981 gatacccgca ggtggcattt cagtcctccg gcggtattgg cgacattaac gatgttgctg
    38041 ccctgcgagg cactggcgtg cgcggcgtaa tagttggtcg tgcattactg gaaggtaaat
    38101 tcaccgtgaa ggaggccatc gcatgctggc aaaacgcata atcccatgtc tcgacgttcg
    38161 tgatggtcag gtggtgaaag gcgtacagtt tcgcaaccat gaaattattg gcgatattgt
    38221 accgctggca aaacgctacg ccgaagaagg tgcagacgaa ctggtgttct acgatatcac
    38281 cgcttccagc gatggccgtg tggtagataa aagctgggta tctcgcgtgg cggaagtgat
    38341 cgacattccg ttctgcgtgg cgggtgggat taagtctctg gaagatgccg cgaaaattct
    38401 ttcctttggc gcggataaaa tttccatcaa ctcccctgcg ctggcagacc caaccttaat
    38461 tacccgcctg gcggatcgct ttggcgtgca atgcattgtg gtcggcattg atacctggta
    38521 cgacggcgaa accggtaaat atcatgtgaa tcaatatacc ggcgatgaaa gccgcacccg
    38581 cgtcactcag tgggaaacac tcgactgggt acaggaagtg caaaaacgtg gagccggaga
    38641 aatcgtcctc aacatgatga atcaggacgg cgtgcgtaac ggttacgatc tcgaacaact
    38701 gaaaaaagtg cgtgaagttt gccacgtccc gctaattgcc tccggtggcg cgggcaccat
    38761 ggaacacttc ctcgaagcct tccgcgatgc cgacgttgac ggcgcgctgg ctgcttccgt
    38821 attccacaaa caaataatca atattggtga attaaaagcg tacctggcaa cacagggcgt
    38881 ggagatcagg atatgttaac agaacaacaa cgccgcgaac tggactggga aaaaaccgac
    38941 ggactaatgc cggtaattgt gcaacacgcg gtatccggcg aagtgttaat gctgggctat
    39001 atgaacccgg aagccttaga caaaaccatc gaaagcggca aagtcacctt cttctcgcgc
    39061 actaaacagc gactgtggac caaaggcgaa acgtcgggca atttcctcaa cgtagtaaat
    39121 attgcgccgg actgcgacaa cgacacgcta ctggtactgg cgaatcctat cggaccaacc
    39181 tgccacaaag gcaccagcag ctgcttcggc gacaccgctc accaatggct gttcctgtat
    39241 caactggaac aactgctcgc cgaacgcaaa tctgccgacc cggaaacctc ctataccgcc
    39301 aaactgtatg ccagcggcac caaacgcatt gcgcagaaag tgggcgaaga aggcgtagaa
    39361 accgcactgg cagcgacggt acatgaccgc tttgagctga ccaacgaggc atcggacttg
    39421 atgtatcacc tgctggtgct gttgcaggat caggatctgg atttaacgac ggtaattgag
    39481 aacctgcgta aacggcatca gtgagttgca gggtaagcgg atgcgatgtt gttgtcgcat
    39541 ccggcaaaaa aaaagggcaa ggtgtcacca ccctgcccgt tttctttaaa gctgcaaaga
    39601 ttacttcgcg ttataattac gcagagcgtt acgccccagc acaatccccg cgccaaccat
    39661 gccgcccagc agcaccgcca gaatcaaggt aattgccttt ttcgggctat cgcgacgaat
    39721 aggtaacgtc ggtttcatca cataacggta agcatgaata tcaagatcat caacctttaa
    39781 gttttcaata tccaacaggt tttgacgtgt ctgatagtag tttggtgaga acaccaacgg
    39841 acgggtcgcc tcatgcttaa tcatcgactc cagcgcttcg ctccccaaaa ggaacaacgt
    39901 atcttgtgtc acatcttcag tctgctgaac ctgtggcttt gccacctgcg cctgattcgc
    39961 atactgcaac gcttcctgaa tctgacggat acgcagatct ttctgctcct gcgcgactac
    40021 ttcctgcgtt cttaaagagt cctgcaagtt tttccgtccc agagcaatgt tgtctttgag
    40081 atccttttct agctcttgat tcactttatc atcaacttgc tgaatgtatt gggccaactt
    40141 catttgtgct ccctctgcag tttgcccaac ataagaaaca gtcaatggta attgctggtt
    40201 cttaacagaa ggttcgatgg taagtttttc tggttcttcc tgattatcca gcgtttctgc
    40261 taatgcagag aaggcagaac tgaagcgacc aattaacgtc tcctgtaaat ccgatacttt
    40321 cggtgcagcc tgaccataga taacattcat ggcattgtta tagccagcaa tttgccccac
    40381 gtcgggctga gtgataattg ctgttgacgt ccatttctcc ttcgctactg ccaaatatcc
    40441 aatagccagg gcaatagcca caatgacgga aatgatgatt gtcatcttgc cacgccacaa
    40501 ctgcgctagt aaatcaatca aatcaatctg ttccgggtca tggttttgcc cagaaacatt
    40561 attattttct actctcatca ttaccctaac tgacggaaat ttccaagtcc gcatagttta
    40621 gcgataatcc tagtgaatgc tataggataa gtgataaaaa taatgaatta ttggggaggt
    40681 aacaaaagac aatcagggcg taaatagccc tgataacaag atgttagtcg ctgccaaaga
    40741 gatcgcgggt gtatacctta tctgccacat ctttaagctc ttctgccatt cggttagaga
    40801 taatgacgtc ggcttgttgt ttgaagatgg cgagatcacg ttccagccga gagttgaaga
    40861 atgagtcttc tttcatcact ggctcgtaga tgatcacttc aacgcctttc gccttgatac
    40921 gcttcataat cccctgaata gaagacgcac ggaagttatc tgaaccgctc ttcataatca
    40981 gacgataaat acccaccact tgcggtttgc gtgacaaaat ggcatcggca ataaaatcct
    41041 tacgcgtgcg gtttgcatcg acaattgccg agatcaggtt attcggcaca gactgatagt
    41101 tcgccagtaa ctgcttggta tctttcggca agcaataacc accataacca aacgacgggt
    41161 tgttgtaatg gttgccaata cgcggatcga gacaaacgcc ttcgataatc tggcgagtat
    41221 tcagacctaa actttctgcg tagctatcca gttcgttaaa gtatgccacg cgcatcgcta
    41281 ggtaggtgtt tgcaaaaagt ttaatcgctt ctgcttcggt cgaatcggtg aacagggtcg
    41341 ggatattttg cttaatcgcg ccttcctgta acaacgcagc aaaacgttct gcgcgttctg
    41401 aacgctcacc gatgacaata cgtgaaggat ggagattatc gtaaagggct ttaccttcac
    41461 ggagaaattc cggagagaaa ataatatttt cagtacgata tttcttatgc atcgcttcgg
    41521 taaaaccaac gggaaccgtt gatttgatga ccataaccgc ataaggattt atctcaacta
    41581 cgtctttaat tactgattct acactggatg tattgaaata attagtttta ggatcatagt
    41641 cggttggagt ggcgatgatg acataatcag catcccggta agcttcattt ttatctaatg
    41701 tggcattaaa gtgtatttta tctgattgca aaaactgctg aatttcctta tcaacaatag
    41761 gagatatccg atcattcagc atagcaacgc gtgacggtaa aatatctaat gccacaacct
    41821 catgattttg tgcgattaga agcccgtttg acaagcctac atagccagta ccggaaatgg
    41881 tgattttcat ttcatgctct cagaattaac ttaactgtga atcatgatgt ttttagcatc
    41941 ctgataagag ctaaatgttt taacgctacg ttattagctg gtcaacgcta cgaaaaacaa
    42001 ttcagatttt ctttataaga atagctgata tttattaagt taatattaag cagtgaaatt
    42061 tagtctataa gataaatggc aaaaaaaaag cccggtgcaa tatacgccgg gcctcaattt
    42121 tactgttggt taaatcagat taatccagcc attcagtatg gaacacacct tctttatcaa
    42181 tgcgcttata agtatgtgca ccgaaatagt cacgctgtgc ctggatcagg ttcgcaggca
    42241 gaacagcggc acggtagcta tcgtaatagg caaccgcagc ggcgaaggtc ggaaccggga
    42301 taccgttctg tactgcataa gcaacgacat cacgcagcgc ctgctggtag tcatcggcaa
    42361 tttgcttgaa gtacggagcc agcagcaggt tagcgatctg cggattttcg gcatatgcat
    42421 cggtgatttt ctgcaggaac tgcgcacgga tgatgcagcc agcacggaaa atcttcgcga
    42481 tttcgccgta gttcagatcc cagttgtatt cttcagacgc agcacgcagc tgagagaagc
    42541 cctgagcgta agaaacgatt ttgcccagat acagcgcacg gcgaactttt tcgatgaact
    42601 cagccttgtc gcctgctgac tgagcttgcg gaccagagag aactttagat gcggcaacgc
    42661 gctgatcttt cagagaagag atataacgtg caaacacaga ctcggtaatc agcgacagcg
    42721 gttctccgag atccagcgcg ctctggctgg tccatttacc ggtacctttg ttagccgctt
    42781 catccaggat cacatcaacc aggtagttac cgtcttcatc ttttttagtg aagatatctt
    42841 tggtgatgtc gatcaggtag ctgctcagtt caccgttatt ccactcggta aaggtctgtg
    42901 ccagttcttc gttagagaga ttcaggccgc ctttcaggag agaataggct tcagcaatca
    42961 gttgcatatc accgtattca ataccgttgt gaaccatctt cacataatgg cctgcgccat
    43021 cagcaccgat ataggtaacg cacggttcgc catcttcagc aacggcggcg attttggtca
    43081 ggatcggcgc aaccagttca taggcttctt tctggccacc aggcatgatg gacggccctt
    43141 tcagcgcccc ctcttctccg ccggaaacac cggtaccgat gaagttaaag ccttcggcag
    43201 aaagctcacg gttacgacga atggtgtcct ggaagaaggt gttaccacca tcgatgatga
    43261 tgtcgccttt atcgagatat ggtttgagcg aatcaatagc agcatccgtg cctgcacctg
    43321 ctttcaccat taacaggatg cgacgaggcg tttccagaga ttcaacaaac tctttcaccg
    43381 tatagtaagg aaccagtttc ttgcctggat tttcggcaat cacttcttcc gtcttttcac
    43441 gggaacggtt gaaaatagag acggtataac cacggctttc gatgttgagc gcaaggttgc
    43501 gccccatcac tgccataccg actacgccga tctgttgctt tgacattgtt tactcctgtc
    43561 agggggtggt caccgccgag tgtgcggctt aaatgtggta tagatgttaa ctcactcata
    43621 gagatggtga atagcattac ctttgctata ccacactgaa tatctcacct tttccgtctt
    43681 ttaaaactta tttatatgaa attactgaat tacttgttca gtaactcaag aataagtttt
    43741 gtcttttctt ccatcagcgg cacatcaccg cgagattcca cattcaaccg caccaccggt
    43801 tcggtgttgg aggtgcgcag gttaaagcgc cagtcggcaa aggtcatgct gatgccatcg
    43861 gtgcgatcca ccgccagcgc ctcgcggcta aaatgctgtt ccacgcggtt aatcgcctca
    43921 aagggttgcg ccagtttgct gttgatctca ccgcttgccg gaaacgctgc catccggtcg
    43981 cgcaccagtt cgcccagcgt ttttcctttc aggcacacca gttcggcgac cagcagccac
    44041 gggatcatgc cgctgtcgca gtaagcgaaa tcacggaagt aatggtgggc gctcatttca
    44101 ccaccgtaga tggcgtcttc cttgcgcatc cgttctttaa taaaggcgtg tccggttttc
    44161 gacatcaccg gcgtgccgcc tgcggcagtc accacatcaa cggtgttcca ggagagacgc
    44221 ggatcgtgga tgatcttcgc gccgggattt ttttcgagga atgcttctgc cagcaggccg
    44281 acaatgtagt agccctcaat aaactgccct ttttcgtcaa acaggaagca gcggtcaaaa
    44341 tcgccatcaa aagcaatgcc catatccgca ccgtgtttga tgaccgcatt gcgggtgtcg
    44401 tcgcggcatt ccggcagtag cgggttagga ataccgttgg ggaaattgcc gtccggcgtg
    44461 ttgtgcactt tgattaattc cacgggtgcg ccaagggctt taaagcgggc ttcgatggcg
    44521 tccaccaccg gacccgctgc gccattcccg gagttaatca ccagcttgag cggcgtgagg
    44581 tttttgacat tgatataacc gaacaggtga tcaacgtaag cgtcacgcag attgatttgc
    44641 tgatagcgac cgcgtttggt ttcatcaacg ggaggaaagt cgttggcttc tgccagacgc
    44701 tggacatcgc gcagtccggt atcaccgctg atcgggcgag ccccttcgcg caccagtttc
    44761 atgccgttgt aatccatcgg gttatggctg gcggtaactt caatgccgcc atccacgccg
    44821 agatggaacg tggcgaaata gatctcttcg gtgccggaca tgccaatatc cagcacatcg
    44881 acgcccgcat cctgtaaacc tctcgccagc gccagtttta aggtttcgct ggtgaggcgg
    44941 acatcaccgc ctaacacaat ggttttcggt ttgagaaatt cgccataggc gcgaccaatg
    45001 cgccaggcga tatcttcatt cagttcttcg cctaatttcc cgcgaatatc ataggcttta
    45061 aagcaggtta attttttcat gtcgttaccc tttttcaggc aatagttggc cctgaccgaa
    45121 acggccaatt ttgtttttgt cgttatttac atctcggttt cgcattcatt gcctgatgcg
    45181 acgtttacac ccgtccgtag cgatccgcga aacgcaccac atcatcctct tcgagataag
    45241 agccggagcg cacttcaatt aaatcgagcg gaattttccc cgggttttcc aggcaatgca
    45301 tcgcccccag cggaatataa atggactcgt tttcaccaag cagtttgata tcaccatcaa
    45361 tggtgacttt tgccgttccc gcgacaacca cccagtgttc cgcgcggtga tggtgcatct
    45421 gtaccgacaa gccctcgccc ggtttcacgg tgatgcgttt cacctggtag cggtcgcccg
    45481 cgtcgataga gtcatatttg ccccacgggc gatagacttc gcgatgcacc cgatgctcat
    45541 ggcgaccatc ggctttgatc tgctcgacca cttttttcac atcctgcacc gcgttacggt
    45601 cggcaatcag caccgcatct ttggtctgca ccactaccag atctttcacc ccgacggtgg
    45661 ttacgaggcc agattcggcg tacacatagc tgttttcagt tttgtgatta atcacatcgc
    45721 cgtggcaaac gttgccctcg gcggtgtggg cgctaatttc ccataatgag gaccaggagc
    45781 cgacatcgct ccagcccgca tccatcggca ccacaacggc atctgccgta cgttccatga
    45841 ccgcgtaatc caccgactct tccgggcagg cgagaaacgc ttcttcatcc acacgaataa
    45901 aatcgagatc cggatcgacg gcgctcatcg ctttttcaca ggcatcgaga atatccggac
    45961 gatatttttt cagttcttcg agatagcgtc cggcgcggaa caggaacatg ccgctgttcc
    46021 agtaatattc gccgcttgcc acataggcct gggcggtttc cagattcggt ttttcgacaa
    46081 actgcgccac ttcaaaggcc accgtatcct gctcacccgc agacacttca ccgcgacgaa
    46141 tatagccata accggtttct ggtagatccg gcacaatgcc gaaggtcacc agcttgcccg
    46201 cttcggcata tggcatggca ttacgcacgg cggcacggaa cgcgtcttca tcggcaatca
    46261 catgatccgc cgccaatacc agcattaacg ggtcgctctc cgggctatga cgttttgccg
    46321 ccagcgccgc cagcgcaatg gcaggtgccg tgtttcgccc tgccggttcg agaataatgt
    46381 tctcggtaag tttgttcagt tgacgcagct gttccgcgac aataaagcgg tgctgctcat
    46441 tgcaaatcac caccgggctt tcgcactcca cgccgttcag gcggcagatg gtggtttgca
    46501 gcatggtgag atcgcctttc aggcataaaa actgcttggg gtaaagtacg cgggaaagcg
    46561 gccataagcg gctaccggag ccacctgcca tcacaactgg atagagtttc gactgcgcca
    46621 taattatccc cgaatatcat ttataaattg acgtaacacg ttctctttat cgagtgtgcg
    46681 ttcggcatat tcacgtgcca ccgtgttgtg tttgggcagc aggagcgcct gacggatccc
    46741 cgccaccagc gcctcgaccg attccggttc tacgcaaacc gcaatgcccg gaaaggtttc
    46801 gcaaagctgc cccagttctg tgtgggcctc tgcggtaatc accgcgttac cgcctaccgc
    46861 cagaatattg gtcagtttcg acggcaatac ggcatctgcc gcgccgcgtt tttgcaccac
    46921 cagatggcaa tcgcccatct tcagcagtgc gggtaaagcg tcatacgatt gcagcgggaa
    46981 aaattgcatg ttgcgcagtc cacgctgctg cgccattttt tccagccgcg ctttgccgcc
    47041 gccctgcccg acaatggcaa aaatcagcgg ttcatcgcgc agacgatcgg cagcttcaat
    47101 aacgttttcc agcccctgct tttcaccaat attgccggag taaagaatga tttttttgtt
    47161 atccggcagg tcaagctggt tacgaagggc gtcaacatcg gcatctgcaa catgctgaaa
    47221 acgggcaatt tccgaccagt tggggaagaa gatgacgttt tccgccgcca cgcctttttc
    47281 gatggcttta ttcatcatcg aacgcgaaat cgtggagacg ttatcgacgt tatgcagtcc
    47341 gctacgttcg aacgccgttg ccagctgtgc cactttgctg cctttgcctt ttccggcaag
    47401 gcccagcccc agcatggcgt ccacttcgta atcctgaata tgcagcacgg tacgcgcacc
    47461 agaaagtttc gccagcaggc gcattcccgg cgtgcaaaac agcgttggca ccacgccgat
    47521 aatgcgatcc ggcttccagc gacgttgcgc catcagagga aagaaactgc tgacggcaaa
    47581 actgccgaga tgtaacaggc gtttcagggt gctcggctgt tttggcacgt acagcgggca
    47641 gcgccacacc gtggcggccc cctcttctcg tttgtaccgc caggcggaat agttctcgcc
    47701 cacctgccac tgcgggtagt aaggcggtgc ggtaataacc cgcacctcat gaccttgtgc
    47761 cgccagccat tccaccatct cgccggtgta tttgccgata ccggttaact ccggcgagta
    47821 gttaatgccg tagaccagta ttttcataat ccgggtactc cggcacgctt ctcagcgaga
    47881 aaataggcgc ggctgttagc gtgaacatcg ttactggcga gcagcgcgtc cggcgtcagc
    47941 cagcggtaat cgtcatgctg ctcatccggc agtatcagct cttcttcagc gactctgaag
    48001 cgaaaaccga gcaccacata gtgagtggtg aaatccgtgc cggagaagtt atcgtcataa
    48061 aagtgctgcc agacaccgta aaactggcct gctgttatcg gcagacgcag ccccagttcc
    48121 gccatcgtca gccgctcgaa tgcggcttcc agcgtttcgt ctttctgcac gcgccctccc
    48181 ggcacaaacc agtaaccctg cgccgggcgg ttggttcttt tgccaagcag aaactcaccg
    48241 cgactgttct cgacaataaa gtcgagagag acaagcgggg tggagcgcac taccgtggca
    48301 aagtcttcct gacgtaaaaa catcattacc cccgaaagcg gtcttgattc tcaaggaacc
    48361 actggtaagt gctggcaagc cccgcttcca gtgagatttc gtgataccag ccaagctgat
    48421 gcaggcgcgt cacatccagc agtttgcgcg gcgtaccatc cggtttgctg gcatcaaaaa
    48481 ccacccgacc tttgtaaccc accactttgg cgatggtttg cgccagctcg cggatagtgc
    48541 agtcaacgcc cgtgccgacg ttaatgtgcg acagcatcgg ctgggtgttc tccagccaga
    48601 cttcgtgcgc cagctccatg acatgaatgc tcgccgccgc catatcatcg acgtgcagga
    48661 attcacgcat cggtgtaccg ctgccccata ccaccacgtc cggtgcattc tgtgccgtcg
    48721 cctcgtggaa gcgacgcagc aatgcaggga tcacatgcga attactcggg tggaagttgt
    48781 catgcgggcc atacaggttg gtcggcatga ctgagcggta atcgcgtccg tactggcggt
    48841 tgtatgattc gcacagtttg atcccggcga ttttggcaat ggcgtaaggc tcgttggtcg
    48901 gctccagcgt gccctgcaat aactcgcttt ctgccatcgg ctgttttgcc agtttcgggt
    48961 agatacagga cgatccaaga aacagcagtt tgttcacgtc gttctgatgc gcggcgtgaa
    49021 tgatgttgct ctcaatcatc atgttctggt agatgaaatc cgccggatag gtgttgttgg
    49081 caacaatgcc gcccactttc gccgccgcca gatagacctg gtcaatgcgt tcgctggcaa
    49141 agaaatcatg caccgcgcgg ctgtccagca ggttcagctc gtcgcgggtg cgtaatacca
    49201 gttccacatc accgcgctgt tcgagctgcc gcctgatggc agaaccgacc atcccacgat
    49261 ggccagcgat aaaaatgcgt tgtttactca tgcttatgac tccagcgcga tcgccacgtc
    49321 gtagccgtga gatttcagca gagagtgttt tttcgccgct tcgaggtcat tagccaccat
    49381 ttcagacacc atctctctga gggtgatttc cggtttccag cccagttttt cgtgcgcttt
    49441 ggtcgggtcg ccgagcagcg tttcaacttc agccggacgg aagtaacgcg gatcaacggc
    49501 gataatcaca tcacccggtt taacgcccgg cgcgtcatgc ccggtgacgg aaaccacaat
    49561 gcccttctct tcaacacccg tgccttcaaa gcgcagtttg atgcccagct gtgccgctgc
    49621 catttccacg aactgacgca cggagtactg aacgccggtc gcgataacga aatcttccgg
    49681 ctgttcctgt tgcagcatca tccactgcat ttttacgtag tctttggcgt ggccccagtc
    49741 acgcagggaa tccatattgc cgaggtacag gcacgactcc agcccctggg cgatgttggc
    49801 gattgcgcgg gtgattttgc gggtaacgaa ggtttcgccg cggcgcgggg attcatggtt
    49861 gaagagaatt ccgttacaag cgtacatgcc gtaggattcg cggtagttaa cggtgatcca
    49921 gtaggcgtac agtttggcga ccgcatacgg agatcgcggg tagaacggcg tggtttcttt
    49981 ctgcggaatt tcctgcacca gaccatacag ttcagaggtg gaagcctgat agaaacgagt
    50041 tttcttttcc agaccgagga agcggatcgc ctccagcagg cgcagcgtac ccatcgcgtc
    50101 gacgtcagcg gtatattctg gtgactcaaa agagaccgca acgtggctca ttgcgcccag
    50161 gttgtacact tcatccggct gtacttcacg caaaatgcgc gtcaggttag aggtatcact
    50221 caggtcgcca taatgcagat ggaatttcgg gttgcaggtg tgcggatcct gataaatgtg
    50281 atccacgcgc tcggtgttga atgacgatgc gcgacgctta ataccatgca cctcgtaacc
    50341 tttttccagc agaaactctg ccaggtaaga accgtcttgt ccggttacac cggtgatgag
    50401 agcgactttt gacatgtctt attcctctgt atttttgaat ttattcagtt tcaacgcgtt
    50461 cgcgtatcac cactgcggga ttcccccggc aaaccacatt tgccggaagc gatttaaaaa
    50521 cactgcttcg tgcacccacg acggtgccgt cgccgattgt gacgcctggg gcaacaaaga
    50581 catcggttgc cagccagcat ttctcgccaa tcacaatagg cgtggcgtta atggtgaaat
    50641 gttgacttgc atggtcgtgg ctaccggtgc ataaataact tttttgcgat atcaccgaat
    50701 gtgcgccaat ggttatttca ccgagggtat ataaattgac gtcatcgccg acccacgcgt
    50761 aatcacctaa ggttaatttc cacggatagg taatttttac tgacggacga ataactacgt
    50821 tttttcctat ttttgctccg aataaacgta ataaaaaagc ccgccagcga tacaatactt
    50881 gtggcgacca ggcaaatatt gttgcctgta ctgcccacca taattgcact ttaatagcgt
    50941 tgccgccccg gaaccctttc ggcaccgaga aaccgcttaa atcttgcatt ggttttcctt
    51001 atattcagac tttgttatat aaggctttcg tctttgaggt agtacgttgg cgtaaatgcc
    51061 aggataattc agcccaaaag ccaggcacat gtaatatttg tcgttggact tttttcgcgt
    51121 cagcacacaa ttccatatta ttggtggtag ataccccacc catggaaaat tcagacacca
    51181 ggccattgag ttttttaaat gcataaccgg ctttatacat tttggctgcc agcgcgtagt
    51241 cggaagaaac tttatattcc aggtcataac gccatttttt caagccggat acagggaaaa
    51301 atatcgcctg atgactggcg ggcaggctgt gataaatata ccagcccggt ttggcgctac
    51361 gtttaatttt atgcccgtcg ccaaaatcca gcagcgcatc gccggtgatc atcatgttat
    51421 ctttttgcat ttttaactta cggacaaaat ttgcggcatc ctgatgaaaa atatcgcccg
    51481 aattgagaaa caacgcgaac ttgccttgtg ccatcgtaat gcctttgttc atggcgtcgt
    51541 agataccgtt atctggctcg ctgacaaagc gtaggttata gataccattg agattttcca
    51601 gatactcacg ggtgccgtca ttggaaccgc catcgacaac aatccattcg aagctgatat
    51661 cttccgcctg cgccagatgc gccagatgcg ccagcgaggc atgtgttttg actatccctt
    51721 cgaggttacg aaacgcgaca gtgattatgc taagcaacat gttcttattc ttacctcgta
    51781 atatttaacg cttttcgcaa aataaacgga cagacgatta aaaatgcata ttccgggcta
    51841 aatatcgaac cggtaaaaaa cagcgatacc ggagtaaaaa gatataattg cacgcgaaaa
    51901 ttgcggttat caccaaaagc gttgatcatc atttttatca ctttccccat gtaccacagc
    51961 gataaaaaca ctgcgaacca ggaaaaataa ataatcagca gatacaaacc attgtctatg
    52021 gtttttccga catccgcacc gttaaatatt ccgaatgatg cgacgtattc ataaagtgag
    52081 ccaaatctga ctacaccgtc aatatgggtc aaagaataac cgaccatcac cagcggaccg
    52141 acaatacgat aatacgatga cgatccttcc gtacctaaat cgcccagacg ggtggaaata
    52201 taaggaaacg cgattaccac accaacccgg aatacagcca gagaaatcaa tgccaacggt
    52261 aactttttct taatcgcctc tttattcaga tattgaaatg cccactccag caaataaaac
    52321 aggataaaag tcataacccc tgaaaacgat ccggataata ttatccctgc gagaatcata
    52381 gcatcggttt taggcgtttt gataccaaac tgtttgatgc tgagccaaat tgagattaat
    52441 gccagagcga aaaatgccgg ttcgaaataa agtgctgtcg tgcgcttgcc gccgaattta
    52501 atgaaattca gtacatagct gttgctgtaa atcagatatt tcgaaatcga ctccataata
    52561 ctgctgccac cggtgaggat aatttgcgcc atctccaccg ccgccagcgc caccaccagc
    52621 ccgatcacca gataaaagaa acgtaatatc ttgcgatggt tgtgtggcga aatcgtttta
    52681 aagcgaatac tccacaccat gccaataatg atcacaatat agacaaacag catggttgaa
    52741 gtgacgtatt tactggcatc cagcgactga ccaaacagat agttaaacgc cgtgagtccc
    52801 gcaccaatcc ctaaggcaat catcaatttt ttaacgctga tgcgttctaa aaacagcagt
    52861 aacaagacag gtaaaaaagt gacgatggtg atggggaaac tttcgccaag ctgggcgatt
    52921 ttgacgttaa ccagcaggta gatcagcggc agcagcaggt agctacagat tctgatagaa
    52981 gttgacatac tcctccagca tctgttgtcc actgtaggtg gcgcggctgc gttggctgaa
    53041 ctcagccagc gtggtaccaa atatcgcctg cgcgatttcc ggtttgctta actgcaccag
    53101 ttgcagcacg tcttcttcgt cgacggtttt accgccggat ttttgcaaca cttcccgtgc
    53161 cgcgtcgcta tgggtggcaa tcaccggcac gccaattgat aacgcctcac acaaaatcag
    53221 cgggtagtta tcgacgcgag aactgaatac cagcgcatcc atctgattga gcgcgctcat
    53281 caacttgcgc ttgtcagttt caaagccgtg attaaccacg ttgccagcgg tgaacggcga
    53341 gaacttacca aaggtatgca gttcgatttt gtcgcccagc gccatcatct cacgcaccag
    53401 ttgctggtta gtcttgccgt cgtaacgcaa gtcatgcgcc accaccgcga ttttcggctt
    53461 gccctgggtt tcgcgcaccg gaggcaagtc cgccagaatc gcttcggttg ccatatcaat
    53521 accattattg ataatccggc aacgccctgg accgtacagg ctattgaaag cgtcagccac
    53581 atgctggctg ggggaaataa actgacagcc cagcgccagc atctcacgga ataactggcg
    53641 tttgcccgcc accagctggt gtgcgcgatc aatcttcacc ggcggatagt tatttaaggt
    53701 cgggcatttc tggcagcccg ttttccagcc ttcgcaaccg tcggtaaagg cgcagcgtcc
    53761 ggtaacactc cagtggtcgt gcagcgtcca gaccagagtg acgtccggtt tatggttttt
    53821 cactttttcg caaaagcgca ccacgctctt aagatttagc cagtagctgt gcagcacatg
    53881 aaaatgcagg accaccggac ccggtgtgcg agtaatggtg cgatataact cattgaaatt
    53941 gccaaacaga tcgcgattaa acagacgaaa cagggcaata ttcgccatcg cggtcatccg
    54001 cggcgtatgt ttgatgacct gcggatagtt ctgatggctg acgctctctt tgccgccttt
    54061 gccataaccg tagacaaaat gtgacgccag cccctgttgc agcgcacgct ggtggagatc
    54121 taacgccaca cctgctgccc cgccttccgc cagtcgcaca ttaaattgca gaatattcat
    54181 ttaattacct tcactcgcgc tttttctccc accaccagcg cgttgtccgg gacagagtcg
    54241 agcaccacgc tgcccgcacc cacggtgacg ttgttaccaa gcgtgatatc accaagaata
    54301 atgacgttgg caccgagttc gacgccgttg ccaatgtgtg gacatgccat gttatcggca
    54361 ccacgattgc cgatagtgac gccgtggcga atggtgaaat catcccccgc taccacgttt
    54421 ttattgatca ctacggcgta accgtgatgg atagtaaagc ggcggccaat ggtcgcggcg
    54481 gcctggattt cataaccgaa aaagcattcg gtgataatgc gatacagcac cagcagcggg
    54541 gccgcccaca gattgttgag gacgttcttt ttgcgccaca ccgaacaaaa atgagcaaca
    54601 cgataggcaa gaaccatgca gcacgggcgt aaactccagc tgttggcacg cagatcttcc
    54661 agcatcttta gcgcccccga ataccatcag ccagacgttt gccgttacgc accgacagca
    54721 gcgtcagcaa agtgcgccag gtcatacgtt tattgcggat ctggtagagg gtaaacagct
    54781 gatatttttt gcttgcacgg tcaaatttat ctttgtgctt tcggtaaaag tggaagtacc
    54841 cggagaattt tttcggtgac gaggtgatct gcatttcacc gtgattgata tgcaggatct
    54901 gcgtcgcttc ttccactttc cacggttcgc cgtactccac caccatccgc aggaagatgt
    54961 cgtaatcctg tgctgctttc agttcggtat cgaacaaaca ctctttgaaa cgccatgccc
    55021 aagtaaagac ctgattgcca atgatattgc gtttatagaa caggcggcgt gagtacggcg
    55081 atttgggata cagtggtaag cttgccggtt gggaataaac ttctccctgg catacgtagt
    55141 cgttagcgta caaaaaggcg tgagtgacca gttgctgttt atgggcgagg aagacgctca
    55201 gacggttggg tgtccattcg tcatcgtcat cgatcccggt aatgtattcc ccttgcgcca
    55261 gcataatcgc ctggttacgt accgcgcacg ccccgctgtt aatgtcgttg tgaatgtagg
    55321 tgatacgcgg atcgttgagg gcagtgacgt actgttgcag ttgttcccag gaggtggagc
    55381 aatcatccac gatgatcatc tcccagttgc tgtagtcctg gcgcaggacc gattttatcg
    55441 cccgaatcgc cagttgttga cggttccagg tcggcatata gattgaaatc agcggattct
    55501 ctttcatagg ttgttctccc ggcttatgag ctggtttgct tgccggatgc ggcgtgagcg
    55561 ccttatccgg cctacggggc ggtgcgaatg caggcgtcgt tatttcgcat ccgacttata
    55621 ttcgtattcg taatagccat aatcctgata cgcgctggcg cggcggaaga tggagttcag
    55681 aatcacccct ttcaccggaa taccgttttg ctcaaagcgg ctcagactgg tttccacttc
    55741 tttcaatgtg ttgaccgcat agcgcgccac cattaacgtg gttccagcgt gacgcccgac
    55801 aattgctgca tcggtcactg ccagaatcgg cggcgtatca atcaacacca ggtcgtagtt
    55861 tttactcgcc cagctcacca attcggcaaa gcgttcgctc atcaacagtt cagaaggatt
    55921 tggcggcacc tgaccgcgcg ggatcaggtc aaatttggca atagaggtcg gtttagctgc
    55981 ggtagtaata tcgccctggc caatcagaat ttccgacagg ccattgacgt tattggtacc
    56041 caacagctcg tgggtgtagc ctttgcgcat atcgcagtcg atcaacaaca cccgtttatt
    56101 ggtctgactg atcaccgccg ccaggttggc gcagacaaag gttttaccga ttgacgggct
    56161 cacccctgtc atcatcaaca cattgttctg cgcctgcatc atcgcgaagt gcagactggt
    56221 acgcagactg cggatggctt caatcgccag atcggttgga ttccccaccg ccagtagctg
    56281 gctctgttta tagcgtttaa tccctttgat ggttttgacg ctatcgcgcg ctttctgcca
    56341 ttccgacagc gggatgctgg catagacgct gataccatgt tcttccagca cctgcgggct
    56401 ttcgatgccg cgattaaaca acgagcgcag cagcacaccc acgatagaga gcatcaggcc
    56461 gaggataatt gcgccgagga taatcagtcc tttcttcggt ttcagcacgc caggctgggt
    56521 gattgccggg tcaacaatgc gcacatcgcc gacggtgctg gcttcggtga ttttcagctc
    56581 ctgctcttta ttcagcagtt gcatatagac ctgctgacca gactcgacat cgcgggtcag
    56641 gcgcacaatc tcctgctggg ttttcggcat cgccgttacg cgaccgttaa gtttggcttt
    56701 ctcttcttcc agcgcctgac gtttctccag cagcgtgcgg tacgccgggt gaactttggt
    56761 gtacagcttg gagatttccg cctctttaaa ggtcagttcg ttcaactgcg cgtcgatgtt
    56821 caccatcgaa tcgagcaccg ctttcgcttc cagcggcaga tcaacagaat ctttatcctg
    56881 acggaaggca ttcagtttgt tttcggcaac atcgaggcgg ctacgtactt ccggtaactg
    56941 ttgcgcgagg aaagcgaggc ttttcgacgc ttccgccgat ttgcgctcaa tattttgttc
    57001 ctgatagtta cgggcgatgc tgttaagaat gtcgcggatc tgttcgcgat cttcaccggt
    57061 ataagtcagg ctcagtacgc ctgcgtcttt gccgttctcc gttaccgtca ggctgttttg
    57121 cagttggttg atcatcccca acgtggagta tttggtgacg gtaaactcac tgcccgggcg
    57181 ggcgtgaatg gcttcaacca tcagcgtgac gccttctttt ttcagcatct ggcccgcttg
    57241 cccacgggcg ctaaagccgc catcactgct cagggtgtag tttttattgt ccagcacatt
    57301 aagcgtaaac acctggcccg ccatctcttt cgggcggtta aaggtagtca ctttcaccgt
    57361 ctcgttctgg cgtcccatca gtcgctccca gcccgccccg aaaatcggga acgtgttttt
    57421 gctcactgca atatcgaggt cgagatcgtc caccgtttta ccaagcacca gacgcgaacg
    57481 aatcaactgg atctcggcgt ccgatgcagg cggtttgttg gctaatgccg aaccaatgtc
    57541 ctgcactaac gaattgccgc tgttttgctc gatttgtacc agtgcgtcgg cactataaat
    57601 cggcgttgcg aagaaggtgt aaaccacggc acagagggca aatacagcgg tgatgccaat
    57661 cacccaccag cgcgcttcaa tgacggtgcc gaccaggcga ccaatatcga tttcatcact
    57721 gcccgttacc ggagcggcat gttgttttac tttttctgtc attcttatac ctgctctgcg
    57781 ttcaatgcct gcgcccactg gcgggcagac cgttcaagta atgtgtacac cgctgcaaac
    57841 gtttcccggc ttttgcgata cggatcgggg atttcacatt cgttatccca gtgaccaaac
    57901 agcatcactt tgccgcgcat ctcaggtgcc atctcgcata agcgttcgat atggcgcttt
    57961 tccatggtca aaatcaggtc atagttgcga cacaggcggc ggctgatttg tcgtgcacag
    58021 tggccttcca gagacagttg atgttctgcg gcgacgctga tagcggtagg atcagcgccc
    58081 ttaccgacca gcgcgccgag tccagcggac tccactttca gttccgggtg ataacgttgc
    58141 agtaagcgtt ccgccgtcgg ggaacggcaa atattgccga cacagacaac taatatgttg
    58201 ttaaacatga cgattaccag ttatgaatgt cgctggctgt atccgtcatg taacggacac
    58261 cgctaatagt tggcaacaac tgattgatca gacggttcca gcgggaaacc ggggcggtgg
    58321 tgacatacac cacgtcataa ggttgcaggc ggaattctgt cgccatcacc agcgacgtgg
    58381 catcggacat atccagctgg tagatattgg caatcttgcc attacgcccg ccctcgcctt
    58441 tcagcggacg aatgacaaag atgccgctgg cgttggaggt ggtcatgtcg ataccttcag
    58501 cattacccag ggcttcagtc agggtcatgc cgctaaagtc cattttcagg gtgctctgtt
    58561 tcttcacttc acccatcaca aatactttca gatcatcatt acgcggcacg tagagaatat
    58621 cgccgggata aagcagtcgg ttctgattga ggtcgccgtt ttgcatcagc gcctgcaaag
    58681 aaatgcgttc ttcacgacca ttgtgtgtta gcactacgtt gcgccagtca gcggtgtcgg
    58741 tcaggccacc tgcggcgttg atggcgtcga gaatagtcag tggcacgttg gtgatcgcct
    58801 gttgaccgga tttattcacc tgaccggaga tataggcctt ttgtgagcgg aaggcggcga
    58861 tattaacgtc cacctgcggg tcagcgatgt acgtcgctaa gcgcccggta atatcactgc
    58921 ggatttcagc gagcgttttc ccgactacgt gaaccttgcc gatatacggg taaaacatag
    58981 tgccgtcagg ctgtacccag ttgccggtgt cgctggagct gcggtactga ccggctggcg
    59041 tggtgagttc cgggtgatcc cagacggtga cattcagaac gtcgcccggc ccgacgcgat
    59101 actggtaatt cgcgatctcg ctttccagcg tcatattggg gcgcgctaca ttcgggcgtg
    59161 ggcgtaattg gtcaatcagg cgcggggtca gcggataaac attcaccatt ttgtcgagat
    59221 cgaaatcagc gtcctgctgt ttgatgacgt ctttgcccat cgtcgacata ttgctgcccg
    59281 gaagtactgt gcaaccgctt atcaaggtta ctgacaccaa taatggcatc aatttcattt
    59341 tggatttcat cattgtttat ttatcacttt ggcagagtaa ttatcctgtg cactattaat
    59401 agcaatatcg ccatgcacat ttaccttgca gttaattgaa taaaaattta actggcatca
    59461 gtcctaaaaa aattgatttc atccgcaggc tattgacaga ataattcaga ctggtctttc
    59521 aggcatccag acacgctacc gcccctggct ttttagccac caacacactg atttagctta
    59581 atttttcaaa ccctctctgc atacaatcgt tgatgagaaa gggttattgc ggaaattaac
    59641 ttccgaatat aaggtgacat tatggtaatt gaatattggc tctccaataa tgcaggagga
    59701 agtgttacag ctaacggaat agcaggcaag gtaacaattc ggcaattggc tatttttaag
    59761 aattatatta agtgtcattc aatatggttt ttaggagttt ctttaggttg acaatattta
    59821 atattgcgcc tccacatgcg atatttctta aataatgttt tattattacc acttttaatt
    59881 cagggataat gtgaggttat taccctcaaa taatatgaga taaatagtgc tgcaacattg
    59941 catttttgcc ccgatttatc catgatcgaa ttgtgacatt tgtcatacaa cgaataggtt
    60001 ttgtacttac tatggaatgg attgccgatc cgtctatctg ggccgggtta atcacgctga
    60061 ttgtgatcga actggtcctc ggcattgata acctggtctt tattgccatc ctcgccgaaa
    60121 aactaccgcc gaggcagcgc gaccgcgcac gggttaccgg gctgctgctg gcgatgttaa
    60181 tgcgcctgtt actgctggcg tcaatctcct ggctggtcac cctgactcaa ccgctgttca
    60241 gcttccgctc gtttaccttt agcgcccgcg acttaatcat gctgtttggt ggtttcttcc
    60301 tgttgttcaa agccacgatg gagctgaacg aacggctgga agggaaagac agcaataatc
    60361 ccacacaacg caaaggtgcg aagttctggg gcgtggtgac gcaaattgtg gtactggacg
    60421 ccattttctc actcgactca gtgattaccg ccgtcgggat ggtcgaccat ttactggtca
    60481 tgatggccgc cgtggttatc gctatcagcc tgatgttgat ggccagcaag ccgttaacgc
    60541 aatttgttaa cagtcacccg acgatcgtta ttctctgctt aagcttcctg ttgatgattg
    60601 gctttagcct ggtggcagaa ggtttcggct tcgtcattcc gaaaggctac ctgtacgctg
    60661 ccattggttt ctcggtgatg atcgaggcgc tcaatcagtt ggctatcttt aaccggcgac
    60721 gttttctttc cgctaaccag acgctgcgcc agcggactac cgaagcggta atgcgcctgc
    60781 ttagcgggca aaaagaagat gcggaactgg acgccgaaac cgcgtccatg ctggtggatc
    60841 atggtaacca gcagatcttt aatccgcagg aacggcggat gattgagcgg gtacttaatc
    60901 ttaaccagcg taccgtcagc agcattatga cgtcgcgcca cgatattgag catatcgatc
    60961 tcaacgcgcc ggaagaagaa atccgccagt tgctggagcg aaatcagcat acgcggctgg
    61021 tggttaccga cggtgatgac gcagaagatt tgctcggtgt tgttcacgtt atcgacctgc
    61081 tacaacagtc tctgcgcggc gaaccgctca acctgcgggt gttgattcgc cagccgctgg
    61141 tgttcccgga aaccttgccg ttgttacctg ccctggagca gttccgtaat gcccgcacgc
    61201 actttgcttt tgtggtggat gagtttggct cggtggaagg gattgtgaca ttaagtgacg
    61261 tcactgaaac cattgtcggt aacttaccga acgaagtgga agagatcgac gcccgccatg
    61321 atattcagaa gaatgcggac ggttcctgga cggcgaatgg tcatatgccg ctggaagatc
    61381 tggtgcaata tgtgccgctg ccgctggatg aaaaacgtga atatcacacc attgccgggc
    61441 tgttgatgga atatttacag cgtattccaa aacctggcga agaagttcag gtgggggatt
    61501 atttgcttaa aacgttgcag gtggaaagcc atcgcgtgca gaaggtgcag attattccgc
    61561 tgcataagga tggcgagttg gagtacgagg tgtgatggta tcggtcattt tacccaatag
    61621 ccgaaaggaa taagatgagg gaaccgcgcc agcggcgatt taccgccggg agcccgggtt
    61681 gccagggtgg tggcgattga gccaccctgg cacgttcacc ggcttgagcg ttccatagta
    61741 gcgaggaaca tcaagtgaac ggaacagcct tcgatcttac atattcagaa tataatgatg
    61801 ccgccacgaa cgcctgatgc gacgctttac gcgtcttatc aggcctacag gactggtcac
    61861 ctggtaacct acatcttctc cagcaacttc ttcacatcct tgccattgcg ggaatcttta
    61921 ttccgctcgg cccagtcatt caggcgacgt ttcgcttcgt cctgtagatg tttgcgcagt
    61981 aactgatcca cttgcaggct gtaattgagt tgctgccagt tgccataaac ccgcagcggt
    62041 actggcgttt ctttcaggaa atcaatcagt ttactttccc cgttccagcc acccacgacc
    62101 cgaatatcaa actgggtatc gcaggtttga tctgccagat tcaacatgcc ttcccctgtc
    62161 agcgccagca caggcgattg accttgcatg tcgtttaacg tcacgacgcc atccttcaac
    62221 gtcaaatcgg tggtaaagcg gtcaagccgc gtcacgttat caaagttttc agcggccttc
    62281 acatcaccgc cattacgctc taccgcttgc tgaatcatct gctggaagtt catcccttcc
    62341 atgcgcgtgt cggtcatttc gacatgtgcc tgtccttgcc agttgtggcg gaatgcgtcg
    62401 gcatctatgt cagcaccgga gaaatcacca gccagtgaca tttttccggt caacgaaatc
    62461 ggatagttaa acgccttcag aatggtacca atctcaacgt tttccagccg tggctggaag
    62521 tttatccgcg gatttattga tgtcgcgtcc agcgtgcccg gcagtgaaac ctgtccaccg
    62581 ttaagtttgc cctgcagttg agtaatttcc agcaaacccg acttgttggt catttgcgtg
    62641 gcaacatctg taaaattcat tccgcgccag cgcacgttac tggcctgcaa caaaatatca
    62701 gccgtaaagc cttgcagtcc ctgataggcc ggttcatcaa tacgcgaaga aatgaccggg
    62761 cgcggcaacg ttgattggct ctgcccctgc tgcgcggcac cgttttcacc attcgcggtt
    62821 tcattaagcg ggatgaggtt gtcaagattc agttgcggga attgcagtct cagctgccat
    62881 tccggtttct ctgtcagcgt gacctgtgct tgcccgctca gcgtactgtc attggcggtc
    62941 aagctgattt ggttaaatga aaggcgttta tgtgactcct gccactgggc ctggaaactc
    63001 ccctgcccct gaataccttg ttttggtaaa tcggcaccct gcaactgcca gttaatttgt
    63061 tcaatagccg ccgttaaatc atgcggataa tcagaagcat ccaccgtacc gttaagggat
    63121 attgtgagat cgcgctgatc gcgattaacc cgcccggaga actcaaatga gccacgatgt
    63181 tggggatctt gttccatttg caggcggata ttgcggattg tcacctgctc gtcatcttca
    63241 tgctggaaca ccagcacgct gtccgccacc ttaagactgg atatatcaaa cgaccatccg
    63301 cgatcgtctg acagatccgg caaggtattg tcgcgcggtg caaccggagc gtcttcactg
    63361 cgcaccgctt ccgtctgcgg cgtcagttgg atcactgccc cttttagcat cacctgctta
    63421 acgctcagtt gatgactcag tagtggtaaa agcgccacgt ccagacgcat gttgtcggcg
    63481 cgaaccagtg gctggcttgc gccctgggcg gtgagagaca ttcgcccgga gaggatacta
    63541 agctgcggcc agacgtgcca acgcagtggc ccgtcgagct gcaattgata accgctacgt
    63601 gcagcaactt gcttgaccat atagtcgcgg aaatcattcg gattcaccag caacactaac
    63661 acagataacc cggccaccag cacgaccagg agtatcatca gcgtcgtcag aaatcgtctc
    63721 atggtatcct caatgggcct gaattagtct ttatcgattc ggctggctac cgcgccctgc
    63781 tggttgcgat atttcgcatc ttcacggcgg ttgtaaggtc gcgccgccgg gccggaaagc
    63841 ggctcaaagc tcagcgcacc aattaacatg cccggacgca gcgccagcgg cagcttaccg
    63901 gagttgtaga actccagcac aatgcaacca gaccagcccg gatcgatacg gtgcgcggtg
    63961 acgtgcacca tcagccccag acgcgccaaa gaggaacgcc cgtccagcca gcccaccaga
    64021 tcggctggca gcgtcaccga ctccagcgtc accgccagcg ccagctctcc tgggtgaaga
    64081 tagaacgcct cgccctcgtc gagaacgatc tcatcgctca tcacgcggtc aagcgcggcg
    64141 ctcacttcat ctttgggacc gctcagatcg ataaacgctg ccgtgtgacc acggaaggta
    64201 cgaaatttat tgcccaggcg tacatccacc gtcgcgccgt taatacgctc cactggcgga
    64261 cgtgggttga tcgacaaacg gccttcatca agccaggctt caatatctcg gtcacacaga
    64321 cgcatggcat tttctccttt cgcgcatcac tcccttaacg ccaattacgt caagggcata
    64381 ccaggttatc actgaacggt acacaattta tcaagcttat tcaaagaact gacttatttt
    64441 cgctttcaat atatcgatcg cgatgcggtt tttcccgccg cgcggcacga taatgtccgc
    64501 atattgttta gaaggctcaa tgaattgcag gaacatcggg cgcacggttt tttgatattg
    64561 cgccatcacg gaatccattg aacgcccacg ctcgttaacg tcacgcttga tgcggcgcat
    64621 caggcagata tccagcgggg tatcaacgaa aatggagaag ttaagttcgt cacgcaaacg
    64681 cgcatccgtc agcaacaaaa tgccttcgag aatgatgacc ttcttcggct caaccgtcac
    64741 cgtttctttc atacgcgtat gttcaacata gctgtaaacc ggcaggtcaa ttgccgagcc
    64801 gcgtttcaac gcttgtaaat gctcaagcag caggctgtga tccatcgcgc tgggatggtc
    64861 gtagttggtc ttaacgcgtt cttccatcga cagatggctt tgatctttgt aatagcagtc
    64921 ttcgggaatt acgccgatgt gttcatcacc gacttgctca cgtaattcac gataaagggt
    64981 actggcaata agactcttgc cggaagccga tgcgccagcg ataccgataa tgacgcactg
    65041 atgagactga tcagtcatat atttagcgac ctgattaacc tggatgttag gaagggggcg
    65101 acgaagcgcc aaacgcggca attataggga tttcatccgt ctgataccag tcgaatagcg
    65161 ttgccgcgcg ctcagagtta attgttgaca aagaattccc ggtggcaaat tacgttgatc
    65221 agttttatgt aaggtaaaaa atgttatacg cagttgcgca aattatccgc ctttacgtca
    65281 ctttatgagc aattcgcata taaaatgtaa aacttttgta ctagcataaa cacagaaacg
    65341 aatactggcg acctggtctt gcggataaag cggtaatgag caaacaatca cagcatgtat
    65401 taattgccct gccccacccg ctgcttcacc tggtcagttt aggtttagtc tcgtttatct
    65461 ttaccctttt ctcgcttgag ctttcgcagt ttggcaccca actcgcccca ctgtggttcc
    65521 cgacgtccat catgatggtg gcgttttatc gccatgccgg gcgcatgtgg ccgggaattg
    65581 cgctgagctg ttcgctggga aatatcgccg catccatcct gcttttttcc accagctcgc
    65641 tgaacatgac ctggacgacc atcaatattg ttgaagccgt ggtcggggca gtgctgctac
    65701 gtaaattgct gccgtggtat aaccccttgc aaaatctggc tgactggctg cgtctggcac
    65761 tcggcagcgc cattgttccg cctctgttgg ggggtgttct ggttgtcctg ctgacgcccg
    65821 gagacgatcc tctcagggca tttttgatat gggtactgtc agaatccatc ggcgctctgg
    65881 cactggtgcc gctgggattg ttatttaaac cacactatct gctgcgccat cgcaacccac
    65941 ggttgctttt tgagtcgctg ctcacgttag ccatcacact gacgttaagc tggctttcga
    66001 tgctgtatct gccgtggcct tttactttca ttattgtgct gttgatgtgg agcgccgtgc
    66061 gcctgccacg aatggaagcc tttttgatct tccttaccac ggtgatgatg gtgtcgctga
    66121 tgatggccgc ggatccctcc ctgcttgcta cgccgcgtac gtacctgatg agccatatgc
    66181 cgtggctacc gtttttgctg atcctgctgc ccgccaacat catgaccatg gtgatgtatg
    66241 cctttcgtgc ggaacgcaaa cacatttccg aaagcgaaac ccgctttcgg aacgcgatgg
    66301 aatattccgc tatcggtatg gcgttagtgg gcaccgaggg acaatggctg caaaccaaca
    66361 aagcgctctg ccagtttctc gggtacagtc aggaagagct gcgcggactc acctttcagc
    66421 aactgacctg gccggaggat ctcaataaag atctccaaca ggttgaaaag ctgataagcg
    66481 gtgaaataaa cacctattca atggaaaaac gctactacaa ccgcaatggc gatgttgtct
    66541 gggcgttgct tgccgtctca ctggtgcgcc acacggatgg cacgccgctc tattttatcg
    66601 ctcagattga agacattaac gagctaaaac gcaccgaaca ggtcaatcag caactgatgg
    66661 agcgcatcac gctggctaac gaagcgggcg ggattggcat ctgggagagg gagttgaagc
    66721 cgaatgtttt tagctgggat aagcggatgt tcgagctgta tgaaattcct ccacatatca
    66781 aaccgaactg gcaggtgtgg tacgagtgcg tgctgccgga agatcgccag catgccgaaa
    66841 aagtgattcg tgattcgttg caatcacgct cgccctttaa actggaattt cgcattaccg
    66901 taaaagacgg tattcgccat atccgcgccc tcgccaaccg ggtactgaat aaagaaggcg
    66961 aagtcgaacg tctcctcggc atcaatatgg atatgaccga agtgaaacag cttaacgagg
    67021 cattgtttca ggaaaaagag cgcctgcaca ttacgcttga ttccatcggt gaagccgtgg
    67081 tctgtattga tatggcgatg aaaattacct ttatgaatcc agtggcggag aagatgagcg
    67141 gctggacgca ggaagaagcg ttaggtgttc cgctcctgac ggtgttgcat attacttttg
    67201 gcgacaacgg accattaatg gagaacattt acagtgccga cacctcacgt tccgcaatcg
    67261 aacaagatgt ggtgttgcac tgtcggagcg gcggcagtta cgacgtgcat tacagtatta
    67321 cgccgttaag tactctggac ggcagcaata ttggttcggt tctggtgatt caggacgtca
    67381 ccgaatcacg caaaatgctg cgccagctga gctacagcgc ctcccatgat gcactgacgc
    67441 atctcgccaa ccgcgccagt tttgagaaac aactgcgtat cctgctgcaa acggtaaaca
    67501 gtacgcatca gcgacatgcc ctggtgttta tcgatcttga tcgctttaaa gcggtgaatg
    67561 acagcgccgg gcatgcggcg ggcgacgctt tgctgcgcga actggcgtcg ttgatgctga
    67621 gtatgctgcg ctccagtgac gtgctggcgc ggctcggcgg tgatgaattt ggtctgctgc
    67681 tgccagactg caatgttgaa agcgctcgtt ttatcgctac acgtattatc agtgctgtga
    67741 atgactatca ctttatatgg gaaggccggg tacatcgggt aggtgccagc gccgggatta
    67801 ccttgatcga tgacaacaat catcaggcgg cagaagtgat gtcgcaggct gatatcgcct
    67861 gttatgcctc caaaaatggt ggacggggcc gggtgacggt ttacgaacca cagcaagctg
    67921 ccgcacatag cgaacgggcg gcgatgtcgc ttgatgaaca gtggcggatg attaaagaga
    67981 atcagttgat gatgatcgcc cacggtgtcg cttcgccgcg gatcccggaa gcgcgtaatt
    68041 tgtggctgat ttcgcttaag ctctggagtt gcgaaggcga aattattgat gaacaaacat
    68101 ttcgtcgtag cttcagcgat ccggcactta gccatgctct tgaccgacgg gtattccacg
    68161 aatttttcca gcaggccgcc aaagcggttg ccagtaaagg cataagcatc gccctccccc
    68221 tttccgttgc cggtttgagt agcgccacgc tggtgaatga tctgcttgag cagctggaaa
    68281 atagccctct accaccacgg ttattacatc tgattattcc ggctgaagcg attttagatc
    68341 acgcagaaag cgtgcaaaaa ctgcagctgg cgggatgtcg gatagtgctc agccaggtgg
    68401 gccgcgatct gcaaatcttc aactcgctga aagcgaatat ggcagattac ctgctacttg
    68461 atggtgagtt atgcgccaac gtgcagggta atttgatgga tgagatgctg attacgatta
    68521 ttcaggggca cgctcagcga ctcgggatga aaaccatcgc cgggccagtc gttttaccct
    68581 tagtgatgga tacgctttct ggcatcggcg tcgatctgat ttatggtgat gtgattgccg
    68641 atgcccaacc gctggatttg ctggtgaata gcagttattt cgcgattaac tgagagagcg
    68701 tttgcccatt gcctgatgcg acgctaacgc gtcttatcat gcctacaaat cgctcattcc
    68761 ccagaccgga taaggcgctc gcaccgcatc cggcgaccaa cgtcatgctt cgtctggttg
    68821 ccagccttcc gtataccaga tatgcaacag cgcataagaa cgccagggct tccagcgctc
    68881 ggcataacgg cggatttgtg ccggtgtcat tcccggaaat cgccgtttaa tcagataatc
    68941 atccggcaga aaaacatctt tcgcctgcca gccacgcaaa gcaaaataat tcgccgtcca
    69001 gcgcccgata cccggaaaag tttgcagcgt tttcatcgcc tgctccacat cgcccggaat
    69061 tgtcattggt aagctgccct ccagcgccgc atttgccaga tgaatcagtg cctctgcccg
    69121 tttcaacggc atgcctaacg cttttaatgc ctgcgggtcg gctgctgcca gccgctgagg
    69181 cgtggggaag cagatatact ccggaaaatc atccagccgt tcgccataaa gctgtaccac
    69241 tttggcagtc aattttgccg ccatcgccac gctcaccagt tggcctaaaa tcgcccgcac
    69301 accctgctca aaagcatcaa tacagccggg taaacgcaat ccgggccgcc ccgcgcctaa
    69361 ctttcccaac gcaccgttaa caatctgcgg gttacattgc agatcaagca ggcggctcat
    69421 tttcgccaga cactctgcgg caacaggttc taaacctgca cttaaattta tgtgcagagt
    69481 atggcgggct atatccggaa tagcagtcac tacgccgcga tattcgccca ccgccagact
    69541 acgggcataa taattgtccg cgaccgtttc cacaccgctc accgcacggg cggcgagaaa
    69601 tcccaacatc cacgaccagt catacggcgg ctgccagttc agggtataca tcgcatctcc
    69661 ttgttaatcc gctttcagca taaacgttat tcagacgtta cgctttgctt tcatattccg
    69721 gttgtcgcga cagcaacatt tcgctaaagt cacgcccctt cttcaccggc atggggatta
    69781 attcgtgttt attggttttg attacggtac agcaaactgt tcagtggcgg tcatgcgtga
    69841 cggtaaaccg caattactga aaatggaaaa cgacagcacg ctgctgcctt caatgctttg
    69901 cgcgccaacg cgtgaatcgg taagcgaatg gctgtaccgc catcatgatg ttccggcaga
    69961 cgacgatgaa acgcaggcgc tgttacgtcg ggcgattcgt tataaccgcg aagaagatat
    70021 cgatgttacg gcgaaaagcg tgcagttcgg tctttcctca ctggcacagt acattgatga
    70081 tccagaagaa gtgtggtttg tgaaatcacc aaaatcgttc ctcggtgcca gcggcttaaa
    70141 accgcagcag gtagcgctgt ttgaggatct ggtctgcgca atgatgttgc acattcgcca
    70201 gcaggcgcag gcgcagctgc cagaagcgat tactcaggcg gtgattggtc gcccgatcaa
    70261 cttccagggg ctgggcggcg atgaagcaaa cgcccaggcg caagagattc tggaacgcgc
    70321 ggcgaagcgt gccggattca gggacgtggt attccagtac gagccgatcg cggctgggct
    70381 ggattacgaa gccaccttgc aggaagaaaa acgggtgctg gtggtggata tcggcggtgg
    70441 tacgactgac tgttcattgc tgctgatggg gccgcagtgg cgttcgcgtc tcgatcgtga
    70501 agccagtctg ctgggtcaca gtggttgtcg tattggcggt aacgatctgg atatcgccct
    70561 ggcgtttaaa aacctgatgc cactgctggg tatgggtggc gaaaccgaaa aaggcatcgc
    70621 cctgccgatc ctgccgtggt ggaatgcggt tgccatcaac gacgtacctg cgcagagtga
    70681 tttctacagt agtgccaacg gtcgtctgct taacgatctg gtacgcgatg cccgcgaacc
    70741 ggaaaaagtg gccctgttac agaaagtctg gcgtcagcgt ttaagctatc gcctggtgcg
    70801 tagcgcagaa gagtgcaaaa ttgctctttc aagcgtagcg gaaacccgcg cctcactgcc
    70861 gtttatcagc aatgaactgg ctacgctgat tagccagcga gggctggaaa gcgccctcag
    70921 ccagccactg gcgcggattc tggaacaggt gcaactggcc ctggataacg cccaggaaaa
    70981 accggacgtt atctacctga ccggcggtag cgcccgttct ccgcagatta aaaaagcgct
    71041 ggcagaacag ttgccgggca ttccgattgc aggcggcgat gactttggct ccgtcaccgc
    71101 cgggctggca cgctgggcgg aagtggtgtt tcgttaattg aaaaatggct cagcagcggc
    71161 ttaaatctgc ctgcgcctga gccagttttt ctgctgcctg acgtaacggt tccatcaacg
    71221 gcatggtccg tgctggagca tgaaggcgga atcgctgcaa ctctgtcgcg ccgactgtca
    71281 acgccgattc cagccgatgt cgtttggcgg tcattttcgc cattaccgct tgtctgtctg
    71341 ctggcgttat cgcttcattc ggcctgaaca caaaacggcg ttcacagttc gctttccagt
    71401 cgataaccgc ctgggtcaga tggtcgccaa accctttcac ttgcttaacg ccacgacggg
    71461 taacatctgc ggctgtttca ataccaaaag accgtaacgc cgctttacgc gcagggccaa
    71521 cgccgggaat agaggcaaca tcaataaaaa atccctccag aaacttctgc ttctgccgtt
    71581 cccttgcagt gtcatgaagt gctgccagag cgcgtttctc ttcttccggt aacccgagaa
    71641 tttcgtcctt cattttttcg agcatcgtcc gtttggcgat aaaaccttcc aggccgccta
    71701 cctgctggat ctggcggact aaatggtcat agtccatttt cgcacgatta aatgcctcac
    71761 ggcgttgctg aacttctgct ttgtacgctt tgcttgtcaa actgccaata atccagatag
    71821 ccgccagcac aggaaccaaa ataatataac gcggttctgc ctgaaggccg caaagcagca
    71881 gcgataatgc tgacagtgcg atctcaagca gaatgatgta ttcgcgccgt aacaggccta
    71941 aaggaagcgg cctgccagtc ggttggaaat gatcgggtaa tggcagttgc aatgctggcg
    72001 gtgctactga cgccatcacc atcgcccaga cttttgccag cacaaaatcc ccgccggtgg
    72061 taatgacctc ttcgccgaga tcaataaaat agataacacc ttgattatcc agcgcacacc
    72121 atgggcagtc agccaaatga ccgggataaa catgcattgc cgaaacggta catttcttta
    72181 actgttggcg tagagaatcc agtgctgata cccacgcttt agccgtcgga cgcccggttg
    72241 ccacgccact ttccgtaaac gcctgctgaa acatggcttc aacatcactc ggtaacatcg
    72301 atagcggaat cgatcgtggc ggcggtttta aaccacgtcg ctgattatct gacgcgtagg
    72361 cataacggaa atgggtaata tccgtctcca gcgcattacc cgcatcagag ataagcggca
    72421 caccagaata aggatgccgg ccaccaaaca agacgtgaaa aatcagcaac gcaaggccaa
    72481 aattatcgtg attttctgtg cgttcaaagc cgacaaatga cgacaaggtt tgtagctctg
    72541 gcggcgtaaa atgcgacacg ccgacttcgc ataaatgcag tgtgccattg gcgttaatct
    72601 gaaaggagtc gctatcaatc aacaccactt tgctgtcgcg acctaccata aaactgttct
    72661 ggtttacgtc acccacgacg tgcccgtgct cgtgaaccgt agcaaaagat gaagcaatat
    72721 tgcgcgcaac atagagtaga aaatcccacg cacaatgagg gtaacgttga cgacgatgtg
    72781 ccgggctata gatcatatga atcggttctt taccagaaac ttttggcatc ataaaaccga
    72841 taacttttcc gcctcgtcca ccgtgaagcg ttgcctgcgg ccaggcgaca taattcaaca
    72901 attgcgcgtc agctgtcgca gccataaagg caagtttgtc ctgttttaag gcgggtggcg
    72961 gcgtgtgata aatcttggcg acgctatcga caaactcatt gatatcataa accgcgcctt
    73021 caccgccttt gcccagttca cggcctggcg taacgcactc accagtagca gtatataaag
    73081 tgggtttcat ggtaaccaca gcgccagtgc caacgtttta tcatcatccg tacgctcgtt
    73141 taccgccggg ctggacaaaa actgctttaa caattcaggt aataaatcga gttgttcctg
    73201 cgttgctgac gccagtccat tgaaaaacgg ggtaaagaaa ggcacatgag gagaattatc
    73261 cagcatattt aacgccagcc gctgaatacc atccgtaaat gctgcaactt tatgcgcacg
    73321 cccagtgctg gtgaaagttt ccagtctgga aacggcatct tcgtcggtaa tgaagtgcgt
    73381 catgttggca tattcaccca ccatcggcgt gagaggcaat tgcagaccgt gacccaaatc
    73441 taccaccacg ccgccatcgc caatctgcat aatcaaggtg ccgtcaggcg atgaaatcaa
    73501 tcccagaaac gtgcaggcaa aatcgcgaac tgctaatgct ttggcttctg cctcggcaaa
    73561 aagtcgttga cgaatagtca gtaccatatt tgtggcgagg acatcattaa gccccaattc
    73621 accgtcctgt actttttgca ccataaacgc catcgcttca ttgacggcga gcgtcgcgcc
    73681 ttctcctccc tgcgaaacgc tacctgcgcc atcagcaagg aacatcacca gtaatggttg
    73741 ctggtcgttt aaccagacaa cctgcatctg gcaggcatcc tgacaaggca aatcagcact
    73801 aatgtgcgat gttccaacag cggatgcata aaccagacgc cagctcacac tgacgtccag
    73861 ccttttggcg cttccagcac aacttccgtt cccggcgtgg agcgggaaac cgaacgcagc
    73921 gaactggata accagctaaa cagttcacgg aattgtaatc cctgcagtgg caaaggctga
    73981 cgaacgctga tttgtgccag agtcttcata tccgccccct gaacaccgat ggaaaagaag
    74041 gcaaacttct tatcttcttc cccctgaaac actttgttgg cggcagcctg ccactcatcg
    74101 gttggagcgc catcggtgat caggaaaatc cacgggcgat aatacgaaat accatttgcg
    74161 cggtactcac gtttcctctc ttcaaccata tccagggctt tggtaatggc ggcccccata
    74221 ggcgtatccc cctgggcaaa caggatgggc gggaaaaagt tagcggcgct ggtaaagggt
    74281 tgttccacat gcaccgggcc gaacgtcaca atgccgagtt ccactctttt taaagccagc
    74341 ggatcagcaa gcagttcatc acgaaaggta accaatccgg cgttaagttc attaataggt
    74401 ctgccgctca tcgagccaga aacatccagc agcaaaatgc aggggcaacg tggttccggg
    74461 ttactggcaa aatcgctggt ggcaaacgtg atttgttcgc tcataagaaa tcctttctta
    74521 gagaattatc gaggtccggt atcataccga ttgcaataat gaaataagag caaacgaaga
    74581 aactttcaga attaggcccg tcggcgcaga aaattttgct attcgcctcg caagaacgga
    74641 gagccgtcga ttgacactaa gggcggagtg acataatttc aggagtgagg gttagggaga
    74701 ggtttccccc tccccctggt gttcttagta agcctggaag ctaatcacta agagtatcac
    74761 cagtatgatg acgtgcttca tcataaccct ttccttatta aaagccctct tctccgggag
    74821 agggtttccc gtttcagcgt cccgctgaaa tcgtcggctt acctcctttc gccctgaatg
    74881 cagtctatcg ctaacgcgtg aacaaaatag tttcatctgt gattattgcg taacgactcg
    74941 caaagaatat ttgctggcag gatcgcagac tacaaagcct gcggattgac aatcttatcg
    75001 tgaaggcata atttcaggag tgagggtaga gcggggtttc ccccgccctg gtagtcttag
    75061 taagcgggga agcttatgac taagagcacc acgatgatga gtagcttcat catgaccctt
    75121 tccttattaa ggccccttcc tcgggagggg ctttcccgtt tcagcgtccc gctgaaatcg
    75181 tcggcttacc tcctttcgcc ctgtatgcag tctatcgctg acttgcggat aaaatagttt
    75241 ccagtgtgat tattgcgtaa cgtctcgcaa agaatatttg ctggcaggat cgcagactgc
    75301 aaagcctgca gattgacaat cttatcgtga aggcatactt tcaggagtga gggtagagcg
    75361 gggtttcccc cgccctggta gccttagtaa gctggccagc taatgactaa gagcaccacg
    75421 atgatgagta gcttcatcat gaccctttcc ttatttatgg ccccttcctc gggaggggct
    75481 ttcccgtttc agcgtcccgc tgaaatcggc ggcttacctc ctttcgccat gcaagcagtc
    75541 tatcgctaac gcgtagataa aatagtttcc tgtgttatta ctggatgcgt gctcgcaaat
    75601 gtgcccgtca ttcagacgat tcccgacagt gtttcataat tcctccattt ttctccctta
    75661 ttggctggct acactagtat cattccgcga aacgtttcag gaagagaaac tcttaacgat
    75721 gaaaggcagt tataaatccc gttgggtaat cgtggtggtt atcgccgcca tcgccgcatt
    75781 ctggttctgg caaggccgca atgactccca gagtgcagcc ccaggggcga cgaaacaagc
    75841 gcagcaatcg ccagcgggtg gtcgccgtgg tatgcgttcc ggcccattag ccccggttca
    75901 ggcggcgacc gccgtagaac aggcagttcc gcgttacctc accgggcttg gcaccattat
    75961 cgccgctaat actgttacgg tgcgcagccg cgtggacggt caactgatgg cgttacattt
    76021 ccaggaaggc cagcaggtca aagcaggcga tttactggca gaaattgacc ccagccagtt
    76081 caaagttgca ttagcacaaa cccagggcca actggcaaaa gataaagcca cgcttgctaa
    76141 cgcccgccgt gatctggcgc gttatcagca actggcaaaa accaatctcg tctcccgtca
    76201 ggagctggat gcccaacagg cgctggtcag tgaaaccgaa ggcaccatta aggctgatga
    76261 agcaagcgtc gccagcgcgc agttgcaact cgactggagc cgcatcaccg caccagtcga
    76321 tggtcgcgtt ggtctcaagc aggttgatgt tggtaaccaa atctccagtg gtgataccac
    76381 cggaattgtg gtgatcaccc agacgcatcc tatcgattta gtctttaccc tgccggaaag
    76441 cgatatcgct actgtagtgc aggcgcaaaa agccggaaaa acgctggtgg tagaagcctg
    76501 ggatcgcacc aattcgaaga aattaagtga aggcacgctg ttaagcctcg ataaccaaat
    76561 cgatgccaca accggtacga ttaaagtgaa agcacgcttt aataatcagg atgatgcgct
    76621 gtttcccaat cagtttgtta acgcgcgcat gttagtcgac accgaacaaa acgccgtagt
    76681 gatcccaaca gccgccctgc aaatgggcaa tgaaggccat tttgtctggg tgctgaatag
    76741 cgaaaacaag gtcagcaaac atctggtgac gccgggcatt caggacagtc agaaagtggt
    76801 gatccgtgca ggtatttctg cgggcgatcg cgtggtgaca gacggcattg atcgcctgac
    76861 cgaaggggcg aaagtggaag tggtggaagc ccagagcgcc accactccgg aagagaaagc
    76921 caccagccgc gaatacgcga aaaaaggagc acgctcctga tgcaggtgtt acccccgagc
    76981 agcacaggcg gcccgtcgcg cctgtttatt atgcgtcctg tcgccaccac gctgctgatg
    77041 gtggcgatct tactcgccgg gattatcggt tatcgcgccc tgcccgtttc ggcgctgccg
    77101 gaagtggact atccgaccat tcaggtggtc acgctctacc caggtgccag cccggatgtc
    77161 atgacctctg ccgttaccgc gccgctcgaa cgccagttcg ggcagatgtc tggcctgaaa
    77221 cagatgtcgt cgcaaagttc cggcggtgcg tcagttatca ctttgcagtt ccagctaaca
    77281 ttaccgctcg atgtcgccga gcaggaagtg caggccgcga ttaacgctgc gaccaacttg
    77341 ttgccgagcg atctgcctaa cccgccggtt tacagcaaag tgaacccggc agatccgccg
    77401 atcatgacgc tcgccgtcac ctcaaccgcc atgccgatga cgcaagtgga agatatggtg
    77461 gaaacccgcg tcgcgcagaa aatctcgcag atttccggcg tcggcctggt gacgctttcc
    77521 ggcggtcagc gtccggctgt tcgcgtcaaa cttaacgctc aggcgattgc cgccctcggc
    77581 ctgaccagcg aaaccgtgcg caccgccatt accggcgcta acgttaactc ggcaaaaggt
    77641 agcctcgacg gcccttcccg tgcggtcacg ctttccgcga acgaccagat gcaatccgcc
    77701 gaagagtatc gccagctaat catcgcctac cagaacggcg cgccaattcg tctgggcgat
    77761 gtcgcaactg tagagcaagg tgcagaaaac agctggctcg gcgcgtgggc gaacaaagaa
    77821 caggccattg tgatgaatgt tcagcgccag cccggtgcta acattatctc caccgccgac
    77881 agcattcggc agatgctgcc acagctcact gagagtctgc cgaaatcggt gaaggtgaca
    77941 gtgctttccg atcgcaccac caatatccgc gcatccgtcg atgatactca gtttgaattg
    78001 atgatggcta tcgcgctggt agtcatgatt atctacctgt ttttgcgcaa tattccggcg
    78061 accatcattc ccggtgttgc tgtaccgctg tcgttaatcg gcactttcgc ggttatggtg
    78121 tttctcgatt tttcaatcaa taacctgaca ctgatggcgt taactatcgc caccggattc
    78181 gtggtcgatg acgccatcgt ggtgatcgaa aacatttccc gctatatcga aaaaggcgaa
    78241 aaaccgttgg cggcggcgct caagggcgca ggtgaaatcg gctttaccat tatctcgctg
    78301 accttctcac tgattgcggt gttgatccca ctgctgttta tgggcgatat cgtcgggcga
    78361 ctgttccgcg aatttgctat taccctggcg gtagcgattt tgatctcagc ggtggtgtcg
    78421 ctgaccctga caccgatgat gtgcgcgcgg atgctcagcc aggagtcgtt gcgtaaacag
    78481 aaccgcttct cccgtgcctc ggaaaaaatg ttcgacagga taatcgccgc ctatggtcgt
    78541 ggactggcga aagtgctgaa tcatccgtgg ctgaccttaa gcgtggcact cagcacgctg
    78601 ctgcttagcg tgctgctgtg ggtgttcatt ccgaaaggtt tcttcccggt acaggacaat
    78661 ggcattattc agggcacttt gcaggcaccg caatccagct cctttaccaa tatggcccag
    78721 cgacaacgcc aggtcgcgga cgtgattttg caggatccgg cagtgcaaag cctgacctca
    78781 tttgttggcg ttgatggcac taacccgtcg ctgaatagtg cacgtttaca aatcaacctc
    78841 aaaccgttgg atgaacgtga tgatcgggtg caaaaagtca tcgcccgtct gcaaacggcg
    78901 gtagataaag tgccgggcgt cgatctcttc ctgcaaccaa cgcaggatct gactattgat
    78961 actcaggtca gccgcaccca gtaccagttt accttgcagg ccacctcact ggatgcgctc
    79021 agtacctggg tgccacagtt gatggaaaaa ctccagcaac tgccacagct ttctgatgtc
    79081 tccagcgact ggcaggacca agggctggtg gcgtatgtca atgttgatcg cgacagcgcc
    79141 agccgtctgg ggatcagcat ggcggatgtc gataacgccc tgtacaacgc gtttggtcag
    79201 cgactgattt ccactattta tactcaggct aaccagtatc gcgtagtgct ggagcacaac
    79261 accgaaatta ccccgggcct cgcggcgctg gataccattc gcctgaccag tagcgacggc
    79321 ggcgtggtgc cgttaagctc aattgccaaa gttgaacagc gttttgcgcc gctttccatc
    79381 aaccatctgg atcagttccc ggtaacgacc atctccttta acgtgccgga taactattcg
    79441 ctgggcgatg cggtgcagga gattatggac accgaaaaga cgctgaatct gccggtggat
    79501 atcaccacgc aattccaggg cagcaccctc gccttccagt cggcgctggg cagcactgtc
    79561 tggctgattg tcgcggcggt ggtggcgatg tatatcgtgc tcggcattct gtacgagagc
    79621 tttattcacc cgatcaccat tctctcgacg ctacccaccg caggggtcgg cgcgctgctg
    79681 gcattaatga ttgccggtag cgaactggat gtgattgcga ttatcggcat tattttgctg
    79741 atcggtattg tgaagaagaa cgccatcatg atgatcgact tcgcgctggc tgctgagcgc
    79801 gagcaaggca tgtcgccgcg cgatgcaatc taccaggctt gtctgttgcg ttttcgtccg
    79861 atcctgatga ccaccctggc ggctctgctt ggcgcgctgc cgctgatgtt gagtaccggg
    79921 gtcggcgcgg aactgcgtcg tccgttaggt atcggcatgg tcggcggtct gattgtcagc
    79981 caggtgctga cgctgttcac cacgccggtg atttatctgc tgttcgaccg cctggcattg
    80041 tggaccaaaa gtcgctttgc tcgccatgaa gaggaggcgt aagtgaagtt ttttgccctc
    80101 ttcatttacc gcccggtggc gacgatttta ctgtcggtcg ccattaccct gtgcggcata
    80161 ctgggcttcc gtatgctgcc ggtcgccccg ctgccgcagg tcgattttcc ggtgattatg
    80221 gtcagcgcct cgctgcccgg tgcgtcacca gaaacaatgg cgtcttccgt tgccacgccg
    80281 ctggagcgct cgcttgggcg cattgccggg gtcagcgaaa tgacctccag cagttcgctc
    80341 ggcagcacac gtattatttt acagtttgat tttgaccggg acatcaacgg cgcagcgcgt
    80401 gatgtgcagg cggcgatcaa cgctgcacaa agtttgctgc ccagtgggat gcccagccgt
    80461 ccgacctatc gcaaagcgaa cccgtcggat gcgccaatta tgatcctcac gctgacatcc
    80521 gatacttatt cgcagggtga actgtacgat ttcgcctcga cgcagctggc tccgacgatt
    80581 tcgcaaatcg acggtgttgg tgatgtcgat gtcggaggca gctcactgcc cgccgtacgc
    80641 gtcgggctga atccgcaggc gctgtttaat cagggcgtgt cgctggacga cgtacgcacc
    80701 gccatcagca atgccaacgt gcgtaaaccg cagggcgcgc tggaagatgg cactcaccgc
    80761 tggcagatcc agaccaatga tgagctaaaa accgccgctg aatatcagcc gttgattatt
    80821 cactacaaca acggcggcgc ggttcgtctg ggcgatgtgg cgacggtgac cgactcagtg
    80881 caggatgtgc gcaacgccgg gatgaccaac gccaaaccgg ctattttact gatgatccgc
    80941 aaactgccgg aagccaatat tatccagacg gttgacagca tccgggcaaa attaccggag
    81001 ttgcaggaaa ccattccggc ggcgattgat ctgcaaattg cccaggatcg ctcccccacc
    81061 attcgcgcct cgctggaaga agtcgagcaa acgctgatta tctcggtggc gctggtgatt
    81121 ctggtggtgt ttttattcct gcgctcgggt cgcgccacta ttattcccgc cgtttcggtg
    81181 ccggtttcgc tgattggtac gtttgcggcg atgtacctgt gcggattcag tctcaataac
    81241 ctttcgttaa tggcgctcac catcgctact ggtttcgtgg tggatgacgc catcgtggtg
    81301 ctggaaaaca ttgcacgtca tctggaagcg ggaatgaaac cgttgcaagc cgcactgcaa
    81361 ggtactcgcg aagtcggttt tacggtgctg tcgatgagtc tgtcactggt ggcggtgttc
    81421 ctgccgctgc tgttgatggg cggattgccg ggccgactgt tacgcgagtt tgccgtgacg
    81481 ctttctgtcg ccattggtat ttcgttgctg gtttctctga cattaacgcc aatgatgtgt
    81541 ggctggatgc tgaaagccag caagccgcgc gagcaaaagc gactgcgtgg ttttggtcgc
    81601 atgttggtag ccctgcaaca aggctacggc aagtcactga aatgggtgct caatcatacc
    81661 cgtctggtgg gcgtggtgct gcttggcacc attgcgctga atatctggct gtatatctcg
    81721 atcccgaaaa ccttcttccc ggagcaggac actggcgtgt tgatgggcgg gattcaggcg
    81781 gatcagagta tttcgtttca ggcgatgcgc ggtaagttgc aggatttcat gaaaattatc
    81841 cgtgacgatc cggcagtgga taatgtcacc ggctttacag gcggttcgcg agtgaacagc
    81901 gggatgatgt ttatcaccct caagccacgc gacgaacgca gcgaaacggc gcagcaaatt
    81961 atcgaccgtc tgcgcgtaaa actggcgaaa gaaccggggg cgaatctgtt cctgatggcg
    82021 gtacaggata ttcgcgttgg tgggcgtcag tcgaacgcca gctaccagta cacgttgtta
    82081 tccgacgacc tggcggcact gcgcgaatgg gagccgaaaa tccgcaaaaa actggcgacg
    82141 ttgccggaac tggcggacgt gaactccgat cagcaggata acggcgcgga gatgaatctg
    82201 gtttacgacc gcgacaccat ggcacggctg ggaatcgacg tgcaggccgc caacagcctg
    82261 ttaaataacg ccttcggtca gcggcaaatc tcgaccattt accagccgat gaaccagtat
    82321 aaagtggtga tggaagtgga tccgcgctat acccaggaca tcagtgcgct ggaaaaaatg
    82381 ttcgttatca ataacgaagg caaagcgatc ccgctgtcgt atttcgctaa atggcaaccg
    82441 gcgaacgctc cgctgtcggt gaatcatcag ggattatcgg cggcctcgac catctcgttc
    82501 aacctgccga ccggaaaatc gctctcggac gccagtgcgg cgatcgatcg cgcaatgacc
    82561 cagcttggtg tgccttcgac ggtgcgcggc agttttgccg gaacagcgca ggtgttccag
    82621 gagacgatga actcacaggt gatcctgatt atcgccgcca tcgccacggt gtatatcgtg
    82681 ctgggtatcc tttacgagag ttacgtacac ccactgacga ttctctccac cctgccctcg
    82741 gcgggcgttg gtgcgctgct ggcgctggag ctgttcaatg ccccgttcag cctaatagcc
    82801 ctgataggga tcatgctatt aatcggcatc gtaaagaaaa acgccattat gatggtcgat
    82861 tttgcgctcg aagcccaacg gcacggtaac ctgacgccgc aggaagctat tttccaggcc
    82921 tgtctgctgc gttttcgccc gattatgatg actaccctgg cggcgctgtt tggcgcgctg
    82981 ccgctggtgt tgtcgggcgg tgacggctcg gagctgcggc acctcctggg gatcaccatt
    83041 gtcggtggac tggtaatgag ccagcttctg acgctgtata ccacgccggt ggtgtatctc
    83101 tttttcgacc gtctgcggct gcgtttttcg cgtaaaccta aacaaacggt aaccgagtaa
    83161 atgacagaac ttcccgacag cacccgctgg caattgtgga ttgtggcttt cggcttcttt
    83221 atgcagtcgc tggacaccac catcgttaac accgcccttc cctcaatggc gcaaagcctc
    83281 ggggaaagtc cgttgcatat gcacatggtc attgtctctt atgtgctgac cgtggcggtg
    83341 atgctgcccg ccagcggctg gctggcggac aaagtcggcg tgcgcaatat tttctttacc
    83401 gccatcgtgc tgtttactct cggttcactg ttttgcgcgc tttccggcac gctgaacgaa
    83461 ctgttgctgg cacgcgcgtt acagggcgtt ggcggtgcga tgatggtgcc ggtcggcagg
    83521 ttgacggtga tgaaaatcgt accgcgcgag caatatatgg cggcgatgac ctttgtcacg
    83581 ttacccggtc aggtcggtcc gctgctcggt ccggcgctcg gcggtctgct ggtggagtac
    83641 gcgtcgtggc actggatctt tttgatcaac attccggtgg ggattatcgg tgcgatcgcc
    83701 acattgctgt taatgccgaa ctacaccatg cagacgcggc gctttgatct ttccggcttt
    83761 ttattgctgg cggttggcat ggctgtatta accctggcgc tggacggcag taaaggtaca
    83821 ggtttatcgc cgctggcgat tgctggcctg gtcgcagttg gcgtggtggc actggtgctt
    83881 tatctgctgc acgccagaaa taacaaccgc gccctgttca gtctgaaact gttccgcact
    83941 cgtacctttt cgctgggcct ggcggggagc tttgccggac gtattggcag tggcatgttg
    84001 ccctttatga caccggtttt cctgcaaatt ggcctcggtt tctcgccgtt tcatgccgga
    84061 ctgatgatga tcccgatggt acttggcagc atgggaatga agcgaattgt ggttcaggtg
    84121 gtgaatcgct ttggttatcg tcgggtactg gtagcgacca cgctgggtct gtcgctgatc
    84181 accctgttgt ttatgactac cgctctgttg ggctggtact acgttttgcc gttcgtcctg
    84241 tttttacaag ggatggtcaa ctcgacccgt ttctcctcca tgaacaccct gacgctgaaa
    84301 gatctcccgg acaatctggc gagcagcggc aacagtctgc tgtcgatgat tatgcaattg
    84361 tcgatgagta tcggcgtcac catcgccggg ctgttgctgg gactttttgg ttcgcagcat
    84421 gtcagcgtcg acagcggcac cacacaaacc gtctttatgt acacctggct tagcatggcg
    84481 tttatcatcg cccttccggc gttcatcttt gccagagtgc cgaacgatac gcatcaaaat
    84541 gtagctattt cgcggcgaaa aaggagcgcg caatgaagtt ctggcgacct ggtattaccg
    84601 gcaaactgtt tctggcgatt ttcgccacct gcattgtttt gctgatcagt atgcactggg
    84661 cggtgcgtat cagttttgag cgtggcttta ttgattacat caagcatggt aatgaacagc
    84721 gattacaact gttaagtgat gcgcttggcg agcagtatgc gcagcatggc aactggcgct
    84781 tcctgcgcaa caatgatcgc tttgtctttc agatcctgcg ttcatttgaa cacgataatt
    84841 cggaagataa acccggcccg ggtatgccac cgcacggctg gcgtacccag ttctgggtgg
    84901 ttgatcaaaa caacaaagtg ctggttggtc cgcgagcgcc gattccacct gacggtacac
    84961 ggcgacccat tctggtcaac ggtgcggaag ttggcgcggt gatcgcctcc cccgttgagc
    85021 ggttaacgcg caatactgat atcaatttcg ataaacaaca gcggcaaacc agctggttga
    85081 ttgtcgccct ggcaacgtta ctcgcggcac ttgccacttt tctgctggcg cgcggtttac
    85141 tggcaccggt aaaacgactt gtcgatggca cgcacaaact ggcggcgggc gatttcacta
    85201 cccgcgtaac gcccaccagt gaagatgaac tgggcaaact ggcgcaagac ttcaaccagc
    85261 ttgccagcac actggagaaa aaccagcaaa tgcggcgcga ttttatggcc gatatttctc
    85321 acgaactgcg tacgccatta gcggtgctgc gcggtgaact ggaagctatt caggatggcg
    85381 tgcgcaaatt cacgccggag acggtggcgt ctttacaggc ggaggtcggt acactgacca
    85441 aactggttga cgatctccat cagttgtcga tgtctgatga aggcgccctc gcctatcaaa
    85501 aagcaccggt agatttgatc ccactgctgg aagtggcggg cggcgcattt cgcgaacgat
    85561 tcgccagccg tggcctgaaa ctgcaatttt ccctgccaga cagtattacc gtatttggcg
    85621 atcgcgaccg tttaatgcag ttattcaata atttacttga aaacagcctg cgctacactg
    85681 acagcggcgg cagcctgaaa atctctgccg agcagcacga caaaacggtg cgcctgacct
    85741 ttgccgacag tgcgccaggt gtcagtgacg agcagctaca aaaattattt gaacgttttt
    85801 atcgcaccga aggttcccgc aaccgcgcca gcggtggttc cgggctgggg ctggcgattt
    85861 gcctgaacat tgttgaagca cataatggcc gcattattgc tgcccattcg ccttttggcg
    85921 gggtaagcat tacagtagag ttaccgctgg aacgggattt acagagagaa gtatgaccga
    85981 gttaccaatc gacgaaaaca caccgcgtat tttgatcgtg gaagatgaac cgaagctggg
    86041 gcagttgctc attgattatc tgcgtgctgc gagctatgcg ccgacgctta tcagccacgg
    86101 cgatcaggta ctgccgtatg tgcgccagac accaccggat ctgatcctgt tggatctgat
    86161 gctccctggc accgatggct tgacgctgtg ccgggaaatt cgtcgttttt ctgacattcc
    86221 tatcgtgatg gtgacggcaa aaatcgaaga gatcgaccgc ctgctcggac tggagattgg
    86281 cgcagatgac tacatctgca agccgtacag cccacgggaa gtggtagcgc gcgtcaaaac
    86341 cattttgcgc cgttgcaaac cgcagcgcga attgcagcaa caggatgccg aaagcccgtt
    86401 gattatcgac gaaggtcgtt ttcaggcttc atggcgcggt aaaatgcttg acctgacgcc
    86461 tgcggaattt cgtctgttga aaacgctctc tcatgaacca ggaaaagtgt tctcccgcga
    86521 gcaattgctc aatcatcttt atgacgacta ccgcgtagta accgaccgca ccattgacag
    86581 ccacattaaa aacctgcgcc gcaagctgga atctctcgac gccgaacagt catttatccg
    86641 cgccgtttac ggcgttggtt accgctggga agccgacgcc tgccgcatcg tttagtttta
    86701 gcgacatgac tttgttagcc ggagatggcg ttcagaccag atccggcaac attatcccac
    86761 gcatggtcag caaactga
//
LOCUS       DAJVQG010000002        78619 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJVQG010000002 DAJVQG010000000
VERSION     DAJVQG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 78619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78619
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.002"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..457
                     /gene="bluF"
                     /locus_tag="OQM83_000087"
     CDS             <1..457
                     /gene="bluF"
                     /locus_tag="OQM83_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HCS1518459.1"
                     /translation="DAVSFLLDEIKANALVPEQIIVEFTESEVISRFDEFAEAIKSLK
                     AVGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITNVHKSGPRQAIIQAIIKCCTS
                     LEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLNGIPSIAWPEKK"
     gene            662..1393
                     /gene="bluR"
                     /locus_tag="OQM83_000088"
     CDS             662..1393
                     /gene="bluR"
                     /locus_tag="OQM83_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HCS1518460.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPHPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            1614..2018
                     /locus_tag="OQM83_000089"
     CDS             1614..2018
                     /locus_tag="OQM83_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HCS1518461.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2071..2181
                     /locus_tag="OQM83_000090"
     CDS             2071..2181
                     /locus_tag="OQM83_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1518462.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLIFEM"
     gene            2417..2461
                     /locus_tag="OQM83_000091"
     CDS             2417..2461
                     /locus_tag="OQM83_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1518463.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            2714..3037
                     /gene="iraM"
                     /locus_tag="OQM83_000092"
     CDS             2714..3037
                     /gene="iraM"
                     /locus_tag="OQM83_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCS1518464.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKKIIWYQSTIFLPPGSIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(3140..4390)
                     /gene="icd"
                     /locus_tag="OQM83_000093"
     CDS             complement(3140..4390)
                     /gene="icd"
                     /locus_tag="OQM83_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCS1518465.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            4562..5215
                     /gene="rluE"
                     /locus_tag="OQM83_000094"
     CDS             4562..5215
                     /gene="rluE"
                     /locus_tag="OQM83_000094"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCS1518466.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDTEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            5225..5686
                     /gene="nudJ"
                     /locus_tag="OQM83_000095"
     CDS             5225..5686
                     /gene="nudJ"
                     /locus_tag="OQM83_000095"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCS1518467.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            5740..6846
                     /gene="mnmA"
                     /locus_tag="OQM83_000096"
     CDS             5740..6846
                     /gene="mnmA"
                     /locus_tag="OQM83_000096"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCS1518468.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            6882..7523
                     /gene="hflD"
                     /locus_tag="OQM83_000097"
     CDS             6882..7523
                     /gene="hflD"
                     /locus_tag="OQM83_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCS1518469.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            7527..8897
                     /gene="purB"
                     /locus_tag="OQM83_000098"
     CDS             7527..8897
                     /gene="purB"
                     /locus_tag="OQM83_000098"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCS1518470.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            9065..9736
                     /gene="phoP"
                     /locus_tag="OQM83_000099"
     CDS             9065..9736
                     /gene="phoP"
                     /locus_tag="OQM83_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCS1518471.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            9736..11196
                     /gene="phoQ"
                     /locus_tag="OQM83_000100"
     CDS             9736..11196
                     /gene="phoQ"
                     /locus_tag="OQM83_000100"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCS1518472.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            11272..12393
                     /gene="roxA"
                     /locus_tag="OQM83_000101"
                     /gene_synonym="ycfD"
     CDS             11272..12393
                     /gene="roxA"
                     /locus_tag="OQM83_000101"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCS1518473.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(12442..13668)
                     /gene="pepT"
                     /locus_tag="OQM83_000102"
     CDS             complement(12442..13668)
                     /gene="pepT"
                     /locus_tag="OQM83_000102"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCS1518474.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            13918..15054
                     /gene="potA"
                     /locus_tag="OQM83_000103"
     CDS             13918..15054
                     /gene="potA"
                     /locus_tag="OQM83_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCS1518475.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            15038..15895
                     /gene="potB"
                     /locus_tag="OQM83_000104"
     CDS             15038..15895
                     /gene="potB"
                     /locus_tag="OQM83_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCS1518476.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            15892..16686
                     /gene="potC"
                     /locus_tag="OQM83_000105"
     CDS             15892..16686
                     /gene="potC"
                     /locus_tag="OQM83_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCS1518477.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            16683..17729
                     /gene="potD"
                     /locus_tag="OQM83_000106"
     CDS             16683..17729
                     /gene="potD"
                     /locus_tag="OQM83_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCS1518478.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(17885..18706)
                     /gene="cobB"
                     /locus_tag="OQM83_000107"
     CDS             complement(17885..18706)
                     /gene="cobB"
                     /locus_tag="OQM83_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCS1518479.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(18722..19633)
                     /gene="nagK"
                     /locus_tag="OQM83_000108"
     CDS             complement(18722..19633)
                     /gene="nagK"
                     /locus_tag="OQM83_000108"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCS1518480.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(19662..20906)
                     /gene="lolE"
                     /locus_tag="OQM83_000109"
     CDS             complement(19662..20906)
                     /gene="lolE"
                     /locus_tag="OQM83_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCS1518481.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(20906..21607)
                     /gene="lolD"
                     /locus_tag="OQM83_000110"
     CDS             complement(20906..21607)
                     /gene="lolD"
                     /locus_tag="OQM83_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCS1518482.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(21600..22799)
                     /gene="lolC"
                     /locus_tag="OQM83_000111"
     CDS             complement(21600..22799)
                     /gene="lolC"
                     /locus_tag="OQM83_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCS1518483.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGNFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            23061..24134
                     /locus_tag="OQM83_000112"
     CDS             23061..24134
                     /locus_tag="OQM83_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCS1518484.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            24262..27708
                     /gene="mfd"
                     /locus_tag="OQM83_000113"
     CDS             24262..27708
                     /gene="mfd"
                     /locus_tag="OQM83_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCS1518485.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVAPRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            27852..28808
                     /gene="ldtC"
                     /locus_tag="OQM83_000114"
                     /pseudo
     CDS             27852..28808
                     /gene="ldtC"
                     /locus_tag="OQM83_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(28891..29148)
                     /gene="bhsA"
                     /locus_tag="OQM83_000115"
     CDS             complement(28891..29148)
                     /gene="bhsA"
                     /locus_tag="OQM83_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCS1518486.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            29389..30021
                     /gene="comR"
                     /locus_tag="OQM83_000116"
     CDS             29389..30021
                     /gene="comR"
                     /locus_tag="OQM83_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCS1518487.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(30083..30622)
                     /locus_tag="OQM83_000117"
     CDS             complement(30083..30622)
                     /locus_tag="OQM83_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCS1518488.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(30849..32153)
                     /gene="ndh"
                     /locus_tag="OQM83_000118"
     CDS             complement(30849..32153)
                     /gene="ndh"
                     /locus_tag="OQM83_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCS1518489.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELYNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMKNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(32561..33103)
                     /locus_tag="OQM83_000119"
     CDS             complement(32561..33103)
                     /locus_tag="OQM83_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1518490.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(33126..34151)
                     /gene="nagZ"
                     /locus_tag="OQM83_000120"
     CDS             complement(33126..34151)
                     /gene="nagZ"
                     /locus_tag="OQM83_000120"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCS1518491.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(34162..34986)
                     /gene="thiK"
                     /locus_tag="OQM83_000121"
     CDS             complement(34162..34986)
                     /gene="thiK"
                     /locus_tag="OQM83_000121"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCS1518492.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(34967..35608)
                     /gene="lpoB"
                     /locus_tag="OQM83_000122"
     CDS             complement(34967..35608)
                     /gene="lpoB"
                     /locus_tag="OQM83_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCS1518493.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVKAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(35622..35999)
                     /locus_tag="OQM83_000123"
     CDS             complement(35622..35999)
                     /locus_tag="OQM83_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCS1518494.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(36002..36361)
                     /gene="hinT"
                     /locus_tag="OQM83_000124"
     CDS             complement(36002..36361)
                     /gene="hinT"
                     /locus_tag="OQM83_000124"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCS1518495.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            36695..38884
                     /gene="fhuE"
                     /locus_tag="OQM83_000125"
     CDS             36695..38884
                     /gene="fhuE"
                     /locus_tag="OQM83_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HCS1518496.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQLDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(38944..40377)
                     /gene="ptsG"
                     /locus_tag="OQM83_000126"
     CDS             complement(38944..40377)
                     /gene="ptsG"
                     /locus_tag="OQM83_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCS1518497.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(40672..41469)
                     /locus_tag="OQM83_000127"
     CDS             complement(40672..41469)
                     /locus_tag="OQM83_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1518498.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(41480..42484)
                     /gene="holB"
                     /locus_tag="OQM83_000128"
     CDS             complement(41480..42484)
                     /gene="holB"
                     /locus_tag="OQM83_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCS1518499.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(42481..43122)
                     /locus_tag="OQM83_000129"
     CDS             complement(42481..43122)
                     /locus_tag="OQM83_000129"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCS1518500.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(43112..44134)
                     /gene="yceG"
                     /locus_tag="OQM83_000130"
     CDS             complement(43112..44134)
                     /gene="yceG"
                     /locus_tag="OQM83_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCS1518501.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(44137..44946)
                     /gene="pabC"
                     /locus_tag="OQM83_000131"
     CDS             complement(44137..44946)
                     /gene="pabC"
                     /locus_tag="OQM83_000131"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCS1518502.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(45066..46307)
                     /gene="fabF"
                     /locus_tag="OQM83_000132"
     CDS             complement(45066..46307)
                     /gene="fabF"
                     /locus_tag="OQM83_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCS1518503.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(46395..46631)
                     /gene="acpP"
                     /locus_tag="OQM83_000133"
     CDS             complement(46395..46631)
                     /gene="acpP"
                     /locus_tag="OQM83_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCS1518504.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(46842..47576)
                     /gene="fabG"
                     /locus_tag="OQM83_000134"
     CDS             complement(46842..47576)
                     /gene="fabG"
                     /locus_tag="OQM83_000134"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCS1518505.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(47589..48518)
                     /gene="fabD"
                     /locus_tag="OQM83_000135"
     CDS             complement(47589..48518)
                     /gene="fabD"
                     /locus_tag="OQM83_000135"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCS1518506.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(48534..49487)
                     /gene="fabH"
                     /locus_tag="OQM83_000136"
     CDS             complement(48534..49487)
                     /gene="fabH"
                     /locus_tag="OQM83_000136"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCS1518507.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(49555..50625)
                     /gene="plsX"
                     /locus_tag="OQM83_000137"
     CDS             complement(49555..50625)
                     /gene="plsX"
                     /locus_tag="OQM83_000137"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCS1518508.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(50706..50879)
                     /gene="rpmF"
                     /locus_tag="OQM83_000138"
     CDS             complement(50706..50879)
                     /gene="rpmF"
                     /locus_tag="OQM83_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCS1518509.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(50931..51452)
                     /gene="yceD"
                     /locus_tag="OQM83_000139"
     CDS             complement(50931..51452)
                     /gene="yceD"
                     /locus_tag="OQM83_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCS1518510.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            51651..52235
                     /gene="yceF"
                     /locus_tag="OQM83_000140"
     CDS             51651..52235
                     /gene="yceF"
                     /locus_tag="OQM83_000140"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCS1518511.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(52347..53306)
                     /gene="rluC"
                     /locus_tag="OQM83_000141"
     CDS             complement(52347..53306)
                     /gene="rluC"
                     /locus_tag="OQM83_000141"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCS1518512.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            53879..57064
                     /gene="rne"
                     /locus_tag="OQM83_000142"
     CDS             53879..57064
                     /gene="rne"
                     /locus_tag="OQM83_000142"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCS1518513.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(57259..58212)
                     /gene="flgL"
                     /locus_tag="OQM83_000143"
     CDS             complement(57259..58212)
                     /gene="flgL"
                     /locus_tag="OQM83_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCS1518514.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(58224..59867)
                     /gene="flgK"
                     /locus_tag="OQM83_000144"
     CDS             complement(58224..59867)
                     /gene="flgK"
                     /locus_tag="OQM83_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCS1518515.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(59933..60874)
                     /gene="flgJ"
                     /locus_tag="OQM83_000145"
     CDS             complement(59933..60874)
                     /gene="flgJ"
                     /locus_tag="OQM83_000145"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCS1518516.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(60874..61971)
                     /gene="flgI"
                     /locus_tag="OQM83_000146"
     CDS             complement(60874..61971)
                     /gene="flgI"
                     /locus_tag="OQM83_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCS1518517.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(61983..62681)
                     /gene="flgH"
                     /locus_tag="OQM83_000147"
     CDS             complement(61983..62681)
                     /gene="flgH"
                     /locus_tag="OQM83_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCS1518518.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(62734..63506)
                     /gene="flgG"
                     /locus_tag="OQM83_000148"
                     /pseudo
     CDS             complement(62734..63506)
                     /gene="flgG"
                     /locus_tag="OQM83_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(63678..64433)
                     /gene="flgF"
                     /locus_tag="OQM83_000149"
     CDS             complement(63678..64433)
                     /gene="flgF"
                     /locus_tag="OQM83_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HCS1518519.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(64453..65658)
                     /gene="flgE"
                     /locus_tag="OQM83_000150"
     CDS             complement(64453..65658)
                     /gene="flgE"
                     /locus_tag="OQM83_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCS1518520.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(65683..66378)
                     /gene="flgD"
                     /locus_tag="OQM83_000151"
     CDS             complement(65683..66378)
                     /gene="flgD"
                     /locus_tag="OQM83_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCS1518521.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVLRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(66390..66794)
                     /gene="flgC"
                     /locus_tag="OQM83_000152"
     CDS             complement(66390..66794)
                     /gene="flgC"
                     /locus_tag="OQM83_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCS1518522.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(66798..67214)
                     /gene="flgB"
                     /locus_tag="OQM83_000153"
     CDS             complement(66798..67214)
                     /gene="flgB"
                     /locus_tag="OQM83_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCS1518523.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            67369..68028
                     /gene="flgA"
                     /locus_tag="OQM83_000154"
     CDS             67369..68028
                     /gene="flgA"
                     /locus_tag="OQM83_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCS1518524.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            68104..68397
                     /gene="flgM"
                     /locus_tag="OQM83_000155"
     CDS             68104..68397
                     /gene="flgM"
                     /locus_tag="OQM83_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCS1518525.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALLNEAQQDLQSN"
     gene            68402..68818
                     /gene="flgN"
                     /locus_tag="OQM83_000156"
     CDS             68402..68818
                     /gene="flgN"
                     /locus_tag="OQM83_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCS1518526.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(68858..70393)
                     /gene="murJ"
                     /locus_tag="OQM83_000157"
     CDS             complement(68858..70393)
                     /gene="murJ"
                     /locus_tag="OQM83_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCS1518527.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(70503..71426)
                     /locus_tag="OQM83_000158"
     CDS             complement(70503..71426)
                     /locus_tag="OQM83_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1518528.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDDALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(71428..72075)
                     /locus_tag="OQM83_000159"
     CDS             complement(71428..72075)
                     /locus_tag="OQM83_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCS1518529.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(72086..72670)
                     /gene="rimJ"
                     /locus_tag="OQM83_000160"
     CDS             complement(72086..72670)
                     /gene="rimJ"
                     /locus_tag="OQM83_000160"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCS1518530.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            72906..74114
                     /gene="mdtH"
                     /locus_tag="OQM83_000161"
     CDS             72906..74114
                     /gene="mdtH"
                     /locus_tag="OQM83_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCS1518531.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            74178..74825
                     /gene="grxB"
                     /locus_tag="OQM83_000162"
     CDS             74178..74825
                     /gene="grxB"
                     /locus_tag="OQM83_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCS1518532.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            74959..75519
                     /locus_tag="OQM83_000163"
     CDS             74959..75519
                     /locus_tag="OQM83_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1518533.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            75625..76671
                     /gene="pyrC"
                     /locus_tag="OQM83_000164"
     CDS             75625..76671
                     /gene="pyrC"
                     /locus_tag="OQM83_000164"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCS1518534.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            76745..76990
                     /gene="dinI"
                     /locus_tag="OQM83_000165"
     CDS             76745..76990
                     /gene="dinI"
                     /locus_tag="OQM83_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCS1518535.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            77280..77534
                     /gene="bssS"
                     /locus_tag="OQM83_000166"
     CDS             77280..77534
                     /gene="bssS"
                     /locus_tag="OQM83_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCS1518536.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            77649..>78619
                     /gene="solA"
                     /locus_tag="OQM83_000167"
     CDS             77649..>78619
                     /gene="solA"
                     /locus_tag="OQM83_000167"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCS1518537.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLP"
BASE COUNT        19331 a        20692 c        19502 g        19094 t
ORIGIN      
        1 tgacgcagtc tcttttttac ttgatgaaat aaaggccaat gctctggtgc ctgaacaaat
       61 catcgttgaa tttactgaaa gtgaagttat atctcggttt gatgagtttg cagaagcgat
      121 taaatcgctt aaggctgtcg gtatcagtgt agcaattgat cattttggcg caggttttgc
      181 cggtttgtta ctcctgtcac gcttccagcc tgacagaatt aaaatcagtc aggaattgat
      241 taccaatgtt cataaaagcg ggccacggca ggcaattatt caagcgatca taaaatgctg
      301 tacatcactt gaaattcaag tcagtgctat gggcgtggca acaccagaag agtggatgtg
      361 gcttgaatcc gcaggaattg agatgtttca gggagatctg tttgctaaag ctaaattgaa
      421 tggtatccct tccatagcgt ggccagagaa aaaataattt tcagcacatt ctttcacatg
      481 atttcagtaa atcatcgtga aaatgaatac gttatgttta aatttaatcc acattaaata
      541 taatgtttca tccaatttag ctgaaatctt atttatactt tgtacaattt tagcgtatat
      601 tgtacaaagt attattgggt cgtgtacagg cggcggagat ttatgaccgc aaggaggaat
      661 tgtggcttat tacagcattg gtgatgttgc tgaacgttgc gggattaatc ctgtcactct
      721 ccgggcctgg caacgccgct acggtttgtt aaaaccacag cgcagtgaag gcggacaccg
      781 actctttgat gaagaagaca tacaacgcat cgaagagatc aagcgttgga taagtaatgg
      841 cgtccctgta ggcaaagtta aagcattact ggaaaccacc agccaggata cggaagatga
      901 ctggaaccgc ctgcaagaag agatgatgtc aattcttcgt atggctaatc ctgccaaact
      961 acgcgcgaga attatttcac tgggtcgaga gtacccagta gatcaattga ttaatcatgt
     1021 ttaccttcct gttcgccagc gtctcgtgct tgatcacaac acctcccgca ttatgagcag
     1081 tatgtttgac ggcgcattaa ttgaatatgc agcagcctca cttttcgaaa tgcgccgtaa
     1141 gcccggtaaa gaagccattc tgatggcgtg gaatgttgaa gagagagcac gattgtggct
     1201 ggaagcatgg cgtttatcat tgtcaggatg gcacatttct gttcttgctg atcccattga
     1261 atcgccgcac ccggaactgt tcccgacgca aacattgatt gtctggacag gcatggcacc
     1321 aacgagaagg caaaacgaac ttttacaaca ttggggtgag caaggttata aagtcatctt
     1381 tcatgccccc taatttcacc tttatcccgc cccttagaca gaatgttata atctgaaccc
     1441 acaacagcat ttgtaccgga gctggtccgt tgcaaaatgc tcaccggaag aattcaggta
     1501 atccctctat cacactctgt acatatcaca gccaaaggac agaccagatt gaagtggtaa
     1561 aaccgatcgt gcacactacg cagtctcaca actaacaaga ggttattatc ctgatggatc
     1621 aggtcgttgt ttttcaaaag atgtttgagc aagtgagaaa agagcaaaac ttctcctggt
     1681 tttattcaga attaaaacat caccgtattg cacattacat ttattatctg gctacggata
     1741 acatcagaat tattactcac gatgacacgg ttttgttatt aagaggaacc aggaacctgt
     1801 taaaagttag tacgaccaag aaccctgcta aaataaaaga ggccgcattg cttcatattt
     1861 acggaaaatc tacatttcgg gaatactgtt caacactggc aggcgcgggc gttttccgtt
     1921 gggttactga tgttaatcat aacaaacgca gttactacgc cattgataat acgcttttat
     1981 atattgaaga tgtagaaaat aataaaccat taatctagct aaagttggat gcttaagaaa
     2041 tgcttcataa ttcagtaagg cattagcata atgggaataa aagtacagag actatcccta
     2101 tggatgataa atactgtcct tttattgcta cccataaata atcaccaggc caatactatc
     2161 aaattgatat ttgaaatgtg atcacttgac tttctatacg ttattttata acggttaata
     2221 tatttataaa aacaacgggt gtgccatacg cccgtttcaa tacttaacgc acatgtgttt
     2281 tggtttagtc atcatccagt tatatgtatt ttagccagga acaggttaaa cctttcctat
     2341 ataactcaaa aattgaaacc ttattctcat gtcatgttta tattcattat tatcgttata
     2401 taaaaaggca accataatgt ttagcaaatt ggcaccaagt agcataaagg ctatgtttta
     2461 attacaggat gttcagtcat ttgaatgtat aacattatag ctaaacaaat ctaaaacgaa
     2521 gtgaataatt tattgttttc actaaatctc attttgttta acatccattg agattccttg
     2581 ctttaaattt tgttttatat aagcaatcgt tttaattaat ttattttttg aaggggtaat
     2641 atactcatat gcaaaataaa gaaatgaaca tccaaatgaa ctatattaaa taccgtggga
     2701 taagacataa caaatgaagt gggtagtaat tgacacggta attcaaccta catgcggcat
     2761 atctttttca gccatatggg gtgatatgaa aaagatcatc tggtatcaat ctactatatt
     2821 tcttcctcct ggaagtatat ttacaccggt taagcttggt attatcctta aggaaaaaga
     2881 atatcctatt actatttata acattgcacc attcaacaag aatttatgga gtttactcaa
     2941 aagcagtcaa gagtgtcctc caggagaaag caaaataaca aataaatgtt tacataatag
     3001 ttgcattata aaaatatgcc catatgggct caagtaatgg gcttcatata aatatgctcg
     3061 atatgctatt aataatatcc acataaataa aacaacgggc gtgttatacg cccgtttcaa
     3121 tatttaactc atggagagat tacatgtttt cgatgatcgc ttcaccaaac tctgaacatt
     3181 tcagcagctt agcgccttcc atcagacgtt caaagtcata agttacggtt ttcgcgttga
     3241 tagcaccttc cataccttta acaattaaat cagccgcttc agtccacccc atatggcgca
     3301 gcatcatctc agcggagagg atgatagagc ctggatttac tttgtcctga ccggcatatt
     3361 tcggcgcagt accgtgggtg gcttcaaaca gggcgcattc gtcaccgatg tttgcgccag
     3421 gggcgatacc gataccgcca acctgcgctg ccagggcgtc agaaatgtag tcaccgttca
     3481 ggttcataca ggcgataaca tcatattcag ccggacgcag caggatctgt tgcaggaatg
     3541 catcagcaat cacgtcttta atgacgatct ctttgccggt gttcgggttt ttaactttca
     3601 gccacgggcc gccgtcgatc agttcaccgc caaactcttc acgcgccagc tggtagcccc
     3661 agtctttaaa cgcgccttcg gtgaacttca tgatgttgcc tttgtgcacc agagtcacag
     3721 agtcacgatc gttagcaatt gcgtattcga tcgctgcacg aaccagacgt ttggtgcctt
     3781 cttccgaaca cggcttaata ccgataccac aatgttccgg gaagcgaatt ttcttcaccc
     3841 ccatctcttc acgcaggaat ttaatcactt tctcggcgtc ggcagagtct gctttccatt
     3901 cgatacccgc ataaatgtct tccgagtttt cacggaagat aaccatatcg gtcagttcag
     3961 ggtgtttaac cgggcttgga gtgccctgat agtaacgtac cggacgcagg cagatgtaga
     4021 gatccagttc ctggcgcagg gcaacgttca gagagcgaat accgccacca acaggagtgg
     4081 tcagcggacc tttaatggca acgcgatatt cacgaatcag atcaagggtt tcagcaggca
     4141 gccagacatc ctgaccataa acctgtgtgg atttttcacc ggtgtaaatt tccatccagg
     4201 agattttacg ctcgccttta taggctttct cgactgcagc gtcgaccact ttcagcatgg
     4261 ctggggttac atctacaccg attccatcac cttcaatgta agggataatc ggattttcag
     4321 gaacgttgag tttgccgttt tgcagggtga tcttcttgcc ttgtgccgga acaactactt
     4381 tactttccat tcacctctcc ttcgagcgct actggtttgc tcgtctgcca ccacgttgca
     4441 tatgcgtttg cgtcctgcga tacggatgct ttagagcaat tttttgttaa tgatttgtaa
     4501 ttggcttgtc aatgctaccc tattgtttgc cgtcatgaaa gatgcgcgtt attaggctat
     4561 aatgcggcaa ttcataatct ctgaaaatac catgcaaaaa acttctttta gaaatcacca
     4621 ggttaagcga ttcagctcgc aacgttctac taggcgtaaa cctgaaaacc agcccacgcg
     4681 tgtgatcctg ttcaataaac cctacgatgt tcttccgcag ttcaccgatg aagccggacg
     4741 caaaacatta aaagaattca tcccggttca gggcgtttat gcagcaggtc gtcttgaccg
     4801 cgataccgaa gggttactgg tgctgaccaa taacggcgcg ttgcaggcgc gtttaaccca
     4861 gccgggcaaa cgcaccggga aaatctatta tgtgcaggtg gaaggtattc ccacacaaga
     4921 cgcacttgaa gccttgcgca atggcgtaac cttaaatgat ggccctaccc tgcccgccgg
     4981 cgcggagctg gttgacgaac cagcgtggtt atggccgcgg aatccaccaa ttcgtgaacg
     5041 caaaagtatt cccaccagct ggctgaagat caccttatat gaaggacgta atcgccaggt
     5101 gcgccgcatg accgcccatg ttggtttccc cacgctgcga ctgattcgtt atgcgatggg
     5161 tgattacact ttggataacc ttgccaatgg cgaatggcga gaagcaacag aataaggatg
     5221 cacaatgttt aaaccgcacg ttaccgttgc ttgcgtagtg cacgcagagg gtaaattttt
     5281 agtcgttgaa gagacgatta atggtaaagc gttatggaac caacctgccg ggcatctgga
     5341 agccgatgaa accttagtgg aagctgccgc ccgtgagcta tgggaagaaa ccggcatcag
     5401 cgcgcaaccg caacacttta ttcgtatgca tcagtggatt gcgccagata aaacgccgtt
     5461 tttgcgtttc ctctttgcca ttgagcttga gcaaatatgc ccgacccagc ctcatgacag
     5521 cgatatcgac tgctgtcgtt gggtcagcgc cgaagaaatt ttaaaggcgt caaatcttcg
     5581 ttcgccgctg gtggcggaaa gtatccgttg ttatcaaagt gggcaacgct atccgctgga
     5641 gatgattggc gattttaact ggccttttac aaagggtgtc atctaaaagg ctgcgtgata
     5701 gaatacgccg ccttgaagtt caatgtcgtg agtgatccaa tgtctgaaac cgcaaaaaaa
     5761 gtaatcgtcg gcatgtccgg cggtgtcgat tcctccgttt ctgcctggct gttgcaacaa
     5821 cagggatatc aggttgaagg cctgtttatg aagaactggg aagaagacga cggtgaggaa
     5881 tattgcacag cggcagcgga tctggctgat gcccaggctg tctgcgacaa gctcggcatt
     5941 gaactgcaca ccgttaactt tgctgccgag tactgggaca atgtcttcga actgttcctt
     6001 gccgaatata aagccggtcg cacgccgaat ccggatattc tgtgcaacaa agagatcaaa
     6061 tttaaagctt tcctcgaatt tgccgccgaa gatttaggtg ccgattatat cgctaccggt
     6121 cattacgtac gtcgggccga tgtcgatggc aagagccgcc tgctgcgtgg tctggacagc
     6181 aataaagacc agagctactt cctttatacg ctcagccatg agcagattgc gcaaagcctg
     6241 ttcccggtcg gcgaactgga aaaaccgcag gtgcgtaaga ttgctgaaga tcttggtctg
     6301 gtcaccgcga agaaaaaaga ctctaccggc atttgcttca ttggcgaacg taaattccgc
     6361 gagttcctgg gccgttatct cccggggcaa ccgggcaaaa tcattaccgt cgatggcgat
     6421 gaaattggtg agcaccaggg gctgatgtat cacactctcg gtcagcgtaa aggtctgggt
     6481 atcggtggca ccaaagaagg caccgaagaa ccttggtatg tggtggacaa agacgtcgaa
     6541 aacaacattc tggttgtcgc tcagggccat gaacacccgc ggctgatgtc tgtcgggttg
     6601 attgcccagc agttgcactg ggtcgatcgc gaaccattca ccggcactat gcgttgcacg
     6661 gtaaaaaccc gctatcgcca gaccgatatc ccttgcaccg tcaaggcgct ggacgatgat
     6721 cgcattgaag tgattttcga tgaaccggtt gccgccgtga cgccgggcca gtctgccgtc
     6781 ttctataacg gtgaagtgtg cctcggtggc ggtattattg agcagcgtct gccgctgccg
     6841 gtctgattat tatctttact taaaacaagg aagcagtgaa cgtggcaaag aattactatg
     6901 acatcaccct cgccctggcc ggtatttgtc agtcggcacg cctggtgcaa caactcgctc
     6961 accaggggca ttgtgatgcc gatgcgctac acgtctcact caacagtatt attgatatga
     7021 accccagctc gacgctggcg gtttttggcg gtagcgaagc caacctgcgc gtcgggctgg
     7081 aaaccctgct cggcgtgctc aatgccagca gtcgccaggg cttaaacgcc gaattaaccc
     7141 gctacacact cagcttgatg gtgcttgagc gcaaactctc ctcagcgaaa ggcgcgctcg
     7201 acactctggg caaccggatc aacggcctgc aacgccagct cgaacacttc gatttacagt
     7261 ccgaaacgct gatgagcgcg atggctgcta tctatgttga tgtgatcagc ccgcttggcc
     7321 cacgcattca ggtcaccggt tcccctgctg tactgcaaag cccacaagtg caggcgaaag
     7381 ttcgcgcaac cctgctggca ggcattcgcg ccgccgtgct ctggcaccag gtcggcggcg
     7441 gacgtctgca actgatgttt tctcgtaatc gcctgaccac tcaggcaaaa caaattcttg
     7501 ctcatttaac cccggagttg tgatctatgg aattatcctc actgaccgcc gtttcccctg
     7561 tcgatggacg ctacggcgat aaagtcagcg cgctgcgcgg gattttcagc gaatatggtt
     7621 tgctgaaatt ccgtgtacaa gttgaagtac gttggctgca aaaactggcc gcgcacgcag
     7681 cgatcaagga agttcctgct tttgctgccg acgcaatcgg ttaccttgat gcaattgtcg
     7741 ccaatttcag cgaagaagat gccgcacgca tcaaaaccat cgagcgtacc actaaccacg
     7801 acgttaaagc ggttgagtat ttcctgaaag aaaaagtggc ggagatcccg gaactgcacg
     7861 cggtttctga attcatccac tttgcctgta cttcggaaga tatcaataac ctctcccacg
     7921 cattaatgct gaaaaccgcg cgtgatgaag tgatcctgcc gtactggcgt caactgattg
     7981 atggcattaa agatctcgcc gttcagtacc gcgatatccc gctgctgtct cgtacccacg
     8041 gtcagccagc cacgccgtca accatcggta aagagatggc aaacgtcgcc taccgtatgg
     8101 agcgccagta ccgccagctt aaccaggtgg agatcctcgg caaaatcaac ggcgcggtcg
     8161 gtaactataa cgcccacatc gccgcttacc cggaagttga ctggcatcag ttcagcgaag
     8221 agttcgtcac ctcgctgggt attcaatgga acccgtacac tacccagatt gaaccgcacg
     8281 actacatcgc cgagctgttt gattgcgttg cacgcttcaa caccattctg atcgactttg
     8341 atcgtgacgt ctggggttat atcgccctta accacttcaa acagaaaacc attgctggtg
     8401 agattggttc ttccaccatg ccgcataaag ttaacccgat cgacttcgag aactccgaag
     8461 ggaatctggg cctttccaac gcggtattac agcatctggc aagcaaactg ccggtttccc
     8521 gctggcagcg tgacctgacc gactccaccg tgctgcgtaa cctcggcgtg ggtatcggtt
     8581 atgcgctgat tgcgtatcaa tccaccctga aaggcgtgag caaactggaa gtgaaccgtg
     8641 accatctgct ggatgagctg gatcacaact gggaagtgct ggctgagcca atccagacag
     8701 ttatgcgtcg ctatggcatc gaaaaaccgt acgagaagct gaaagagctg actcgtggta
     8761 agcgcgttga cgccgaaggc atgaagcagt ttatcgacgg tctggcgttg ccagaagaag
     8821 agaaagcccg cctgaaagcg atgacgccgg caaactacat tggtcgcgcc atcaccatgg
     8881 ttgatgagct gaaataaact cgtatcagtg ccggatggcg atgctgtccg gcctgcttat
     8941 taagattatc cgctttttat tttttcactt tacctcccct ccccgctggt ttatttaatg
     9001 tttaccccca taaccacata atcgcgttac actattttaa taattaagac agggagaaat
     9061 aaaaatgcgc gtactggttg ttgaagacaa tgcgttgtta cgtcaccacc ttaaagttca
     9121 gattcaggat gctggtcatc aggtcgatga tgcagaagat gccaaagaag ccgattatta
     9181 tctcaatgaa catttaccgg atattgcgat tgtcgatctc ggattgccag acgaggacgg
     9241 tctgtcactg attcgccgct ggcgtagcaa tgatgtttca ctgccgattc tggtattaac
     9301 cgcccgtgaa agctggcagg acaaagtcga agtattaagt gctggtgctg atgattacgt
     9361 gactaaacca tttcatattg aagaggtgat ggcgcgaatg caggcattaa tgcggcgtaa
     9421 tagcggtctg gcttcacagg tcatttcgct accaccgttt caggttgatc tctctcgccg
     9481 tgaattatct attaatgacg aagtgatcaa actgaccgcg ttcgaataca ccattatgga
     9541 aacgttgata cgcaataatg gcaaagtggt gagcaaagat tcgttaatgc tccaactcta
     9601 tccggatgcg gagctgcggg aaagccatac cattgatgta ctgatgggac gtctgcgcaa
     9661 aaaaattcag gcacaatatc cgcaagaagt gattactacc gttcgcggcc agggctattt
     9721 gttcgaattg cgctgatgaa aaaattactg cgtctttttt tcccgctctc gctgcgggta
     9781 cgttttctgt tggcaacggc tgcggtagta ctggtgcttt cgcttgccta cggaatggtc
     9841 gcgctgatcg gttatagcgt cagtttcgat aaaactacgt ttcggctgtt acgtggcgag
     9901 agcaatctgt tctataccct tgcgaagtgg gaaaacaata agttgcatgt cgagttaccc
     9961 gaaaatatcg acaagcaaag ccccaccatg acgctaattt atgatgagaa cgggcagctt
    10021 ttatgggcgc aacgtgacgt gccctggctg atgaagatga tccagcctga ctggctgaaa
    10081 tcgaatggtt ttcatgaaat tgaagcggat gttaacgata ccagcctctt gctgagtgga
    10141 gatcattcga tacagcaaca gttgcaggaa gtgcgggaag atgatgacga cgcggagatg
    10201 acccactcgg tggcagtaaa cgtctacccg gcaacatcgc ggatgccaaa attaaccatt
    10261 gtggtggtgg ataccattcc ggtggagcta aaaagttcct atatggtctg gagctggttt
    10321 atctatgtgc tctcagccaa tctgctgtta gtgatcccgc tgctgtgggt cgccgcctgg
    10381 tggagtttac gccccatcga agccctggca aaagaagtcc gcgaactgga agaacataac
    10441 cgcgaattgc tcaatccagc cacaacgcga gaactgacca gtctggtacg aaacctgaac
    10501 cgattgttaa aaagtgaacg cgaacgttac gacaaatacc gtacgacgct caccgacctg
    10561 acccatagtc tgaaaacgcc actggcggtg ctgcaaagta cgctgcgttc tctgcgtagt
    10621 gaaaagatga gcgtcagtga tgctgagccg gtaatgctgg agcaaatcag ccgcatttca
    10681 cagcaaattg gctactacct gcatcgtgcc agtatgcgcg gcgggacatt gctcagccgc
    10741 gagctgcatc cggtcgcccc actgctggac aatctcacct cagcgctgaa caaagtgtat
    10801 caacgcaaag gggtcaatat ctctctcgat atttcgccag agatcagctt tgtcggtgag
    10861 cagaacgatt ttgtcgaggt gatgggcaac gtgctggata atgcctgtaa atattgcctc
    10921 gagtttgtcg aaatttctgc aaggcaaacc gacgagcatc tctatattgt ggtcgaggat
    10981 gatggccccg gtattccatt aagcaagcga gaggtcattt tcgaccgtgg tcaacgggtt
    11041 gatactttac gccctgggca aggtgtaggg ctggcggtag cccgcgaaat caccgagcaa
    11101 tatgagggta aaatcgtcgc cggagagagc atgctgggcg gtgcgcggat ggaggtgatt
    11161 tttggtcgcc agcattctgc gccgaaagat gaataaatat gtccatactt cacgcattac
    11221 gttaagcatc cgttataatc ggttgcagat accagcctgt ggatgcttaa catggaatac
    11281 caactcactc ttaactggcc cgattttctt gaacgtcact ggcagaaacg cccggtggtg
    11341 ttaaaacgcg gctttaataa ttttattgac ccgatctctc cagacgagtt ggcgggtctg
    11401 gcgatggaaa gcgaagttga cagtcgactg gtcagtcacc aggatggcaa atggcaggtc
    11461 agccacggcc cgttcgaaag ctacgatcat ctcggtgaaa ccaactggtc attactggta
    11521 caggcagtga accactggca tgagccgacc gccgcgctga tgcgaccgtt ccgtgaacta
    11581 ccggactggc gtattgatga tctgatgatt tctttttctg tacctggcgg cggcgtcggc
    11641 ccgcatctcg atcagtacga cgtgtttatc attcagggta ccggacgtcg tcgctggcga
    11701 gtgggcgaaa agctgcaaat gaaacagcac tgcccacatc cggatctgtt acaggtcgat
    11761 ccgttcgaag ccatcatcga tgaagagctg gagcctggtg atattcttta tattccgcca
    11821 ggattcccgc atgaaggcta cgcgctggaa aatgcgatga actattccgt gggctttcgc
    11881 gcgccaaata cgcgggaact gattagtgga tttgccgatt atgtgctgca acgtgaactg
    11941 ggcggcaact actacagcga tcctgatgtt ccacctcgcg ctcatcctgc ggatgttctg
    12001 cctcaagaga tggataaact gcgtgagatg atgctcgaat tgatcaacca gccggaacac
    12061 tttaagcaat ggtttggcga gtttatatcc cagtcacgtc atgaactgga tatcgcgccg
    12121 ccagaaccgc cttatcagcc agatgaaatc tacgatgcgc tgaaacaagg tgatgtgctg
    12181 gtgcgcctgg gtggtctgcg cgtattgcgc attggcgacg acgtgtatgc caatggtgag
    12241 aagatcgatt ccccgcaccg tccggcactg gatgcactcg ccagcaacat tgcgctgact
    12301 gcggagaatt ttggcgatgc gctggaagat ccgtcattcc tcgcgatgct cgcggcgctg
    12361 gtcaatagcg ggtattggtt cttcgaaggg taagtttgga tttaagccgg atgcggcatc
    12421 acatgccgca tcccttttcg cttacttccg ttgcgccgtt aactcggcaa tacggacgat
    12481 cacctgcacc gctttttcca taccttccag agtcacaaac tcatgcttac catgatagtt
    12541 gtaaccgcca gtgaacaagt tcgggcacgg taatcccata aacgacaact gcgcgccgtc
    12601 ggtaccaccg cggatcggtt tcagttccgg ttcaatatcg cagtcacgca tcgcctgctg
    12661 ggcgatatcg agaatatgcg gatgctcaac cactttctct cgcatattgt agtaactgtc
    12721 ttcaatcacc agttcaatgt agcaatcagg atgtaaccct ttgcccactt ttttggcgat
    12781 ctccatcatt ttacgtttac gcgcttcaaa ctgtttacgg tcgaaatcac ggatgatgta
    12841 gtgcatatcg gcccgttcaa cggtgccttt catgctcgcc aggtgataga aaccttcata
    12901 gccttctgtc atttccgggc tttcatccgc cggaacttcc gcatgaatac gtgccgccag
    12961 cgacagcgca tttaccatca ctcctttcgc cgtgcccgga tgaacattgt taccgacaat
    13021 tttgatattg accgatgcgg cgttgaagtt ttcaaactcc agttcgccta cgccaccacc
    13081 gtcaacagtg taagcccagc gggcatcgaa ggcatcaaca tcaaaatgtt tcgccccttt
    13141 gcccacttct tcatccgggg taaaggcgac gcgaatatca ccatgcggaa tgtttttctg
    13201 ttgcaatacc gccagcgcgg tcatgatttc tgcaatacct gctttgtcat cggcacctaa
    13261 caaggtttta ccatcggtgg taatcaacgt ctgacccagt agctgatgca gcaccgggaa
    13321 cataaccggt gataaaactt catcgccgat acccagcgca atatcgccac cgcgatagtt
    13381 ttcaacaatt tgcggattaa catttttgcc gctgcaatcc ggtgaggtat ccacatgaga
    13441 aataaagcca atcgccggga tatcgccagg gacgttagcc ggtaacgtcg ccatcaaagt
    13501 gcccttctca cttaaggtca cattgataag ccccatttct tcgagctgct ctttcagcag
    13561 atgcaataac ttccattggc cttccgtgct gggaacctgt ctcacccctg cttttgattg
    13621 ggtatccaga gatacgtagt tcaaaaatcg ctcaagtagt ttatccatgt agtcaccctc
    13681 actttttgtg acaacattat taagaagcaa gaaaagacaa atattgcgtc aggtcacttt
    13741 tacccctgca agcgggaata tttatcagca ttaccttaat gaatataaag ctaagccagt
    13801 aattcacaac aaagattggc gattcaagcg gtttgccgta gaattaccgc cctcattaag
    13861 agtcaccaag gtggttaacc acaaaccccg catcggtaag ccatccgttg cgtttacatg
    13921 ggacagagta aaaaattgaa taaacaaccg agttcgcttt caccgctggt gcaattggcg
    13981 ggaattcgca aatgctttga tggtaaagag gtcattcccc agctggatct gactatcaac
    14041 aatggcgagt tcctcacgct gcttggccct tctggctgcg gtaaaacaac cgttcttcgc
    14101 ctgattgcag gtctggaaac tgttgattcc ggacgcatca tgctggataa cgaggacatc
    14161 acccacgttc cggcggaaaa ccgctatgtg aacactgttt tccaaagcta cgcacttttc
    14221 ccccacatga ccgtgttcga aaatgtggcc tttgggttgc gcatgcaaaa aacccccgct
    14281 gctgaaatta cgccccgcgt gatggaagcc ctgcggatgg tgcaactgga aaccttcgct
    14341 caacgtaaac cgcatcagct ctctggtggt caacagcaac gcgtcgccat tgctcgcgcg
    14401 gtggttaaca agcctcgtct gttgttgctg gatgagtcgc tctcagcgct ggattacaaa
    14461 ctgcgtaagc aaatgcagaa cgagctgaaa gcgttacagc gtaagcttgg tattaccttc
    14521 gtctttgtca ctcacgatca ggaagaagcg ctcaccatgt cagacaggat tgtggtgatg
    14581 cgcgagggtc gtattgagca agatggtaca ccgcgcgaaa tttacgaaga gccgaaaaac
    14641 ctgtttgttg ccggcttcat tggcgaaatc aatatgttta acgccactgt catcgaacgt
    14701 ctcgacgagc agcgcgtacg cgccaacgtt gaaggccgcg aatgtaatat ctacgttaac
    14761 ttcgccgttg aaccggggca aaaactgcat gttctgctgc gcccggaaga cttacgtgtt
    14821 gaagagatta acgacgacaa ccacgctgaa gggctgattg gttacgttcg tgagcgtaac
    14881 tacaaaggca tgacgctgga gtcggttgtt gaactggaaa atggcaagat ggtgatggtc
    14941 agcgaattct tcaatgaaga cgaccctgac tttgaccact ctctcgacca aaaaatggcc
    15001 attaattggg tagaaagctg ggaggtcgta ctggctgatg aagaacacaa gtaagttcca
    15061 gaatgtagtg attgtcacta ttgtcggttg gcttgtgttg tttgtctttc tgcccaacct
    15121 gatgatcatt ggcaccagct ttttgacccg cgacgacgcc agtttcgtca aaatggtctt
    15181 tacgctggat aactacacgc gtctgctcga tccgctctat tttgaagtgc tattgcactc
    15241 gctgaatatg gcgctgatcg ccaccctcgc ctgcctggtg ctgggctacc cgtttgcctg
    15301 gtttctggcg aagctgccac acaaggtgcg tccgctgctg ctgtttctgc tgattgttcc
    15361 cttctggacc aactcactga ttcgcatcta cgggctgaaa attttcctca gcaccaaagg
    15421 ctatctcaac gagtttttgc tctggctggg cgttatcgac acaccaattc gtatcatgtt
    15481 cacgcccagt gcggtgatta tcggtctggt ttacattctg ctaccgttta tggtgatgcc
    15541 gctgtactcc agtatcgaaa aactggataa gccgttgctt gaggcggcgc gcgatctcgg
    15601 tgccagcaag ttacagactt ttatccgtat cattattcca ctgacgatgc cgggaattat
    15661 tgccggatgt ctgctggtga tgctgccagc gatgggcctg ttctatgtat ccgacctgat
    15721 gggcggtgcg aaaaacctgc tgatcggtaa cgtcatcaag gtccagttcc tcaatattcg
    15781 tgactggccg tttggtgcag ccaccagcat tacgctgact atcgtaatgg gcctgatgtt
    15841 gctggtttac tggcgcgctt ctcgtctgct gaataaaaag gtggaactcg aatgatcggt
    15901 cgactgcttc gcggcggttt tatgaccgct atctacgcgt acctgtatat cccaatcatt
    15961 attttgattg tgaactcctt taacagctcg cgctttggca tcaactggca gggttttacc
    16021 accaaatggt atagcctgct gatgaacaac gacagcctgt tacaggcagc gcagcattca
    16081 ctaacaatgg cggtgttttc ggcgacattt gctacgctta tcggttcact gacggcagtt
    16141 gcgctgtacc gttatcgctt tcgtggtaag ccgttcgtta gcggaatgct gtttgtggtg
    16201 atgatgtcac cagatatcgt gatggcgatt tctctgctgg tgctgtttat gctgctgggt
    16261 attcagcttg gcttctggtc gctgctgttc tcgcatatca ccttctgcct gccgtttgtg
    16321 gtggtgacgg tgtattcgcg cctgaaaggt tttgacgtgc ggatgctgga agcggcgaaa
    16381 gatctcggtg ccagtgaatt taccattctg cgaaaaatca ttctgccact ggcaatgcca
    16441 gcggtggcgg cgggctgggt gttaagcttt accctgtcga tggacgatgt ggtagtttct
    16501 tcgttcgtca ccggaccaag ttatgaaatt ctgccgttaa aaatttattc gatggtcaaa
    16561 gtcggcgtat cgccggaagt taacgcgctg gcaaccatat tactggtgct gtcgctggtg
    16621 atggttattg ccagccagct tattgctcgt gataaaacga aaggtaacac aggggacgtt
    16681 aaatgaaaaa atggtcacgc cacctgctcg cggcgggtgc tctggcactg ggcatgagcg
    16741 ccgctcacgc cgatgacaac aacacgctgt atttctacaa ctggaccgag tacgtgccgc
    16801 caggactgct tgaacagttc accaaagaaa ccggtattaa ggctatctat tcgacttacg
    16861 agtcgaacga aaccatgtac gccaagctga aaacgtacaa agacggtgcc tatgacctgg
    16921 tggttccttc aacctattac gtcgataaaa tgcgtaaaga agggatgatc cagaagatcg
    16981 acaagtcgaa gttaacgaac ttcagcaatc tcgatccaga catgctcaac aagccgtttg
    17041 acccgaataa cgactactcc attccgtata tctggggtgc gacggcgatt ggcgttaacg
    17101 gtgatgcggt ggatcctaaa tctgtcacca gttgggcaga tctgtggaag ccagagtaca
    17161 aaggtagcct gctgctgacc gacgatgccc gtgaagtgtt ccagatggcg ctgcgcaaac
    17221 tgggctactc cggtaacacc accgatccga aagagattga agctgcatat aacgagctga
    17281 aaaaactgat gccaaacgtc gcagcgttta actccgataa cccggctaac ccgtacatgg
    17341 aaggcgaagt taacctcggc atgatctgga acggctctgc tttcgttgca cgccaggcgg
    17401 gtacgccaat tgacgtggtg tggccgaaag aaggcggcat tttctggatg gacagcctgg
    17461 cgatcccggc aaatgccaaa aacaaagaag gcgcgctgaa attgatcaac ttcctgctgc
    17521 gcccggatgt ggcaaaacag gttgccgaaa ctatcggtta tccaacgcca aaccttgcgg
    17581 cgcgtaagct gttaagcccg gaagtggcga acgataaaac gctctacccg gatgctgaaa
    17641 ccattaaaaa tggtgaatgg cagaatgacg ttggcgcagc cagcagcatt tatgaagagt
    17701 attatcagaa gctgaaagca ggacgttaat tcagcaaacg ggcggtggta ttaccgcccg
    17761 ttttttagtt agcaaggatg tggctaatga gtcaggacag taaagtttta tcccgctctt
    17821 tcgtcggtat tgtggtcccc ttgtaggcct gataagcgta gcgcatcagg caatgcttcc
    17881 tgctttataa tcccttcagc aacttttcaa caaactcagg caccacctgg cttgccgggc
    17941 cgtaatattt ctcggcaaat tcattaccga cctgacttgg ttcgagattc agttccacgg
    18001 tgtgcgcgcc atgcagtttc gcttcgtgaa caaacccagc cgccggataa acatgcccgg
    18061 aagtaccaat ggcaatgaaa atatcagcca tcgacaacgc catataaatt tcatccatgc
    18121 cgagtggcat ttcgccaaac caaacgacgt gcgggcgcaa gggtgccggg aactggcaac
    18181 aatggcattt atcttctggc gtaacgtctc cggtccagtc gagaacctga ccactttgcg
    18241 aacaacgaac tttcagcaat tccccatgca tatgaatgac attggtatta cctgcgcgtt
    18301 catgcaggtt gtctatattc tgcgttacca gcaaaaagcg atcgccgagg gcgtcttgca
    18361 gtttagccag cgcaagatgc gcggcgttag gctgaatttc tggctgctgc agctgtcgac
    18421 gacgggcgtt ataaaacgct tgcaccagtt caggatcgcg atcgaaacct tccggcgttg
    18481 ccacatcttc aacccgatgt tcttcccaca ggccatctgc ggcgcgaaag gtacgaatac
    18541 ctgattccgc agaaattcct gcccctgtca gtacgagtac tcttggtttt tccattgctt
    18601 ccggcaccac tttatctctg aaaaaaatac gctgacgcaa acgctcgcgc aggcggcgtt
    18661 tatttttacg aaaacgactt aaccgatgac cccgacgcga cagcatagca acctctgttg
    18721 tttaatcggt tagatgtagg aaggccgcac cacgcattcc tcccgcatca ccgtggcgcg
    18781 cgcgttcaat gcgcggaaca cgagctacag gtaagagatg acgaggcagc ctgtccgcca
    18841 gttgcgttgt gattgccggg aaattcgata agccaccacc aatgacgacc aggtcagagt
    18901 caacgatggt caggatattt cccagacaaa ccgctaataa atccagataa cgctcaacgt
    18961 gcgcccttgc ctgctcatcg ccttgatcat aaagcgcaat aatttcggga gcctgcaacg
    19021 gttgatgata atagtgttgc cagagccacg caaagccccg acctgataga taattttcga
    19081 tacaaccaat ttgaccacag ccgcagcggc gtaacgggaa atccagcccc atcatggtta
    19141 acgcatcaac cggcagacgc atatggccaa actcgccggt aatgtagctt ttaccggtaa
    19201 tcggtttacc gttgaaaatc agcccgccgc caacgccggt gccgagaatt aaccccatca
    19261 ccagtggata ttgcgtaaat tcatcatccc aggcttctga aagggcaaaa cagttggcat
    19321 cgttatcaag gcgtacatcg cgatcaagac gtgcgctcag gtcggcacgc agcggtttac
    19381 cgctggcagc ggggacattg gcggcataca gcgtaccatc ttctgtttcc ggcattcccg
    19441 gaataccgat gccaacagaa cctttacagc caaaacgttg gtcagcttcg gctaccagct
    19501 cgcatactgc atctaaaaat gcgtcatagc tgtcacgcgg tgtcggcacc cgcttttccc
    19561 actgcaactg ccgaccgcta tcaaacacgc caagcgcaat ttttgttcca ccaatatcaa
    19621 acccgtaata cattgccgct ccttgtttta atgtactgcc tttactggcc gctaaggact
    19681 cgcgcagggt caatattact ggcgcgccgc gccggatacc aacttgccaa aagactcagc
    19741 aacaatgctg tgaccagtac gtagaagacg tccagccagt gcaattccga tggcaggaag
    19801 tcaataaaat agatatcgct ggagaggaac tgatgaccga tcagcttttc aatccgctca
    19861 ataatcgggg taagttgcag tgaaacaact acgccgataa tcacaccaca caggctaccg
    19921 aacagccctg ccagcaatcc ataccagaca aagatggcgc gaattaaacc atctttcgcc
    19981 cccagcgttc ttaatactgc gatatcgcca ctcttgtctt tcaccgccat cactaaggtg
    20041 gagacgatgt tgaaacaggc cacgccaatc accagtacca tcgccagata cataatggcg
    20101 cggatcatct ggatatcgcg atacatatag ccgtaagtac caatccaact tttaatataa
    20161 acatagctgt tggtcacttc acccgcatcg cgtaccagct tattggcgtt gaaaacatcc
    20221 gtcattttaa gggcaatacc tgacacgctg gaacccatat caagatattg ttgggcatcc
    20281 gccagcggga tcatggcaaa actgtgatcg agttgaccac tcaactgcaa aataccggca
    20341 atgtgcaaac gcacacgttt tggctgcatc agtttatgct cgggattcga gttgggaatc
    20401 atgatcgaca cccaatcgcc ctgcttcact ttcagcgcat ccgccacgcc tttgccgatg
    20461 ataatttgct gttcgcccgc tttaaaattg cgccaggcat ccccctgaac aaacgagggt
    20521 aatgcgctca gacgctgttc ctgttgcggg ttaacgccct tcacctgaat tgcgcgcaga
    20581 ttggtaccac tttctaccag tccggtgaaa ttgatatacg gcgcagcggc ggcaataccc
    20641 ggcacttttt gcacgttatc cagtgcttcc tgccagttag tccatggctg atttaccgcc
    20701 tcaatttcac cgtgcggtac cacagcaaga atgcggttat tcagttcgcg ttcaaagccg
    20761 ttcatcgcgc ttaagccgac gatcaatacc gccacgccga gggcaatgcc aatggtagaa
    20821 atgacagaga ttagcgacac catgccgccg cgtcgccgtc cgcgactaaa acgcaggcca
    20881 atcaataacg ataaaggcat cgccattact ccgcccccat caggctcagt tccgccgtca
    20941 gacgtccatc acgcatttcc agttggcggc tcatacgttt cgccagttgc aggtcgtgag
    21001 taaccaccag gaaggcggtg ccctgcaagc gattcaattc cccaagcaac tggaagatgc
    21061 tgtcggcgtt acgcgcatcg aggttaccgg taggctcatc cgcaagtacc agacgcggat
    21121 tattgaccag cgcacgggca atcgccacac gctggcgttc gccgccagaa agttcagatg
    21181 ggcggtgatt cgcacgatgc tccagcccca ccgcttttaa catctccagc gcacggctgt
    21241 tgatttcagc gggctttttc ttgccaatca gcagtggcat agccacgttt tccagggcag
    21301 taaaatccgg cagcaggtgg tgaaactgat aaataaagcc cagcttctgg ttgcgcagtt
    21361 ctgctttcgc cgccgaagac agtttgctca tcggttgacc gttaaagatc acatcgccgg
    21421 aggttggtgt atccagcccg cccagcaggt gcagcaaggt acttttaccg gaaccagagc
    21481 taccgacgat cgccatcatt tcaccttcgc cgacgctgaa actgacattg tgcagtacat
    21541 cggtttgcac actgccttcc tgatagcgtt tgcacaggtt gtcgcattgc aacaggatct
    21601 tattcataac gtaaagcctc agcgggttga gtggcggcag cgcgccatga agggtaaagc
    21661 gtagacagca gcgcgatagc catcgccacc agcgcaataa caatgacctg taaaggttcg
    21721 atagcgaccg gcagcgccgc gccatcaagc aggacgccga ttatcggcat cagattattt
    21781 aactggctgg caagcagtgc gccaagcgcc gctccgagga tcgcaccgat aatcccggcg
    21841 ctggcccctt gcaccataaa gaccatcatg atttgtcgcg gagttaagcc ttgcgtttgc
    21901 aggatcgcga cttcgccctg cttctccatt accatcagcc ctagtgaggt aataatgtta
    21961 aacgccgcaa cggcgacaat caggctcagc agcaagccca tcatattttt ttccatgcgt
    22021 acggcctgga acagctcgcc tttacggtca cgccagtcct gccatttgct gccttcaggc
    22081 agtttttgct gacttaaaga gtcaactttc agcggctcat ccagccacaa acgccagccg
    22141 gtaatattgc ctgccggata acgcatcaga cgcgaggcat cctcaatatt caccagcatt
    22201 tcatagccat cgacttcact gttggcggcg aaattgccaa tcacattgaa caggcgctgg
    22261 cttggaatac gccccatcgg cgtgaactgg ctggcagatg gtaccatcac gcggatttga
    22321 tcaccgcgat taacgcctag ctgtgaggca agttgttcgc cgaggatgac attatatttc
    22381 cccggctcga ggtcagtttg tttcacattg accagatacg gcgtaagtgg atctttttgc
    22441 gccgggtcga taccgagcat caccccgacc gccacgctgc gcgcgctttg cagtaccaca
    22501 tcaccggtag taataggtgc gacgcgatta acgccgtcca gtttgactgc cgtttccggg
    22561 agttgctgcg ggttaagaga gccatgctca gaagagagaa ttgcctgtgg catcaggcca
    22621 aggatgttgt tttgcagctc gcgctcaaag ccgttcatca ctgacaatac tgtgaccagc
    22681 gccatcaccc cgagggtaat gccgatggta gaaagccagg agacgaaacg accgaagcga
    22741 tccgctgcac gcccacgcat gtaacgcagg ccaataaata gagcgacagg ttggtacatg
    22801 aaatccgtct ggttgctgta gcaaagacac gagtatataa gcgaaagcaa gatgggtaaa
    22861 cgtctgaact gtaagtgatc gtcagggcta atctgaaaag cccccgtaaa tcaatcggct
    22921 aaattgccat gttttatcat caatcttgcc gcctgatctt aatctgaaaa atcatccaat
    22981 acagacagat acccattaca cctcgggtca taacccgcag gatcaaccgc atgtaaatta
    23041 aaaaagagcg tgttgtcctg atgaaacaaa aagagctatg gattaaccag atcaaagggt
    23101 tatgtatttg tctggtggtg atttatcact cggtcattac cttttatccg catatgacca
    23161 cttttcagca tccgttatcg gaagtcctga gtaaatgctg gatctatttc aatctttacc
    23221 ttgcaccctt tcgtatgccg gtttttttct ttatatccgg ctatttgatt cgccgctata
    23281 tcgacagcgt accgtgggga aattgtctcg ataaacggat ctggaacatc ttctgggtgc
    23341 tggcgctttg gggcgtggtg cagtggctgg cgttaagtgc gctgaaccag tggctggcac
    23401 ctgagcgcga tttaagcaat gcctccaatg ccgcttatgc cgattccacc ggtgagttcc
    23461 tgcacgggat gatcaccgcc agcaccagct tgtggtatct gtatgcgtta attgtctatt
    23521 tcgtggtatg taaaattttt agccgtctgg cgctgccact attcgccttg tttgtactgc
    23581 tgagtgtggc ggttaatttc gttcccacgc cgtggtgggg aatgaacagt gtgatccgca
    23641 atttgcctta ttacagcctt ggcgcatggt ttggcgcaac aataatgacc tgtgttaaag
    23701 aggtgccgtt gcgccgccat ctgctgatgg cttctttgct taccgttctg gcggtcggtg
    23761 cctggttgtt tactatctcg ctgctgttgt cgctggtatc gattgtggtg atcatgaagc
    23821 tgttttatca gtacgagcaa cgattcggta tgcgctccac cagcctgctg aatgtgattg
    23881 gctccaacac cattgctatc tacaccaccc atcgcattct ggttgaaata ttcagcttaa
    23941 ctctgcttgc gcaaatgaac gcagcacgct ggtcgccgca agtcgaactg acactcctgc
    24001 tggtttatcc ctttgtcagt ttgttcatct gtactgttgc gggcttgctg gtaagaaaac
    24061 tttcacagcg tgcattcagc gatctgttgt tctccccgcc ttcactgccc gctgccgtca
    24121 gttactctcg ttaaaccatc gccccagccg gggcgattca atttgctaaa ccatgtcgat
    24181 tacggataat gaacagcatt gcttatcaga gatatgcccc catatgttga ggcatatcct
    24241 aacgagaatc tgacaaccgt tatgcctgaa caatatcgtt atacactgcc cgtcaaagcg
    24301 ggtgagcagc gtctgctggg cgagttaacc ggcgcagcct gtgcaacgct ggtagcggaa
    24361 attgccgaac gtcacgccgg tccggtggta ctcattgcac cagatatgca aaatgctctg
    24421 cgtttgcatg atgaaatcag ccagttcacc gatcagatgg tgatgaatct ggcggactgg
    24481 gaaactcttc cctacgacag tttttcgcct catcaggaca ttatctcctc gcgcctttcc
    24541 accctttacc agctaccgac gatgcagcgt ggcgtactga ttgttccggt gaatacgctt
    24601 atgcagcgcg tttgcccaca cagttttctc cacggtcatg cgctggtgat gaaaaaaggt
    24661 cagcgcctgt cacgagatgc attacgaacc caactggaca gcgccggtta tcgccatgtt
    24721 gaccaggtga tggagcacgg cgaatacgcc acgcgcggcg cgttgctgga tctcttcccg
    24781 atggggagtg agctgcctta tcgtcttgat ttctttgatg atgaaatcga cagcctgcgg
    24841 gtgtttgacg tcgacagcca gcgcacgctg gaggaagtag aagcgatcaa tctgctgccc
    24901 gcgcacgaat ttccgaccga taaagcggca attgaactgt tccgcagcca gtggcgcgat
    24961 accttcgaag tgaagcgcga tccggaacat atttaccagc aagtgagtaa aggcacatta
    25021 cctgccggga tcgagtactg gcagccattg ttcttcagcg aaccactgcc gccgctgttc
    25081 agttatttcc ctgccaatac cttgctcgtg aatactggcg atctggaaac cagtgccgaa
    25141 cgtttccagg ctgacacgct ggcgcgtttt gagaatcgcg gcgtcgatcc gatgcgcccg
    25201 ctgttgccac cacaatcgct ctggctgcgg gtggacgagc tcttctcaga gctgaaaaac
    25261 tggccccggg tgcagctaaa aactgaacat ttaccgacaa aagccgcgaa tgccaattta
    25321 ggtttccaga aactgccaga cctggccgtt caggcacaac aaaaagcgcc gctggatgcg
    25381 ctgcgtaagt tcctcgagac tttcgacggt ccggtggtgt tctcggtaga aagtgaaggt
    25441 cgccgtgaag cgctgggtga actgctcgca cgaattaaaa ttgctccgca acgcattatg
    25501 cgtcttgatg aagccagcga ccgtgggcgt tatctgatga ttggcgctgc cgaacatggt
    25561 tttgtcgata aggtgcgtaa tctggcgctg atctgcgaaa gcgatctgct cggtgaacgt
    25621 gttgcccccc gtcgtcagga ttctcgccgc accatcaacc ccgatacact gatccgtaac
    25681 cttgcggagc tgcatattgg tcagccggtg gtccatctgg agcacggtgt cgggcgctat
    25741 gccggaatga ccacgctcga agcgggcggc attactggcg agtatttgat gctcacctat
    25801 gccaacgacg ccaaactgta tgttccggtg tcgtcactgc atctgattag ccgttacgca
    25861 ggtggcgcgg aagaaaacgc cccgctgcat aaacttggcg gcgatgcgtg gtcacgcgcg
    25921 cggcagaaag cggcggaaaa agtgcgtgat gtggcggcgg aattgctgga tatctacgcg
    25981 caacgcgccg ccaaagaggg cttcgcgttt aaacacgatc gtgagcagta tcagttgttc
    26041 tgcgacagct tcccgtttga aaccacgccg gatcaggcgc aggccattaa tgcggtactt
    26101 agcgacatgt gtcagccgct ggcaatggat cgtctggtgt gcggcgatgt tggctttggt
    26161 aaaacagaag tggcgatgcg cgccgctttc ctggcagtag ataaccacaa gcaggtggcg
    26221 gtgttggtgc ctaccaccct tctcgcgcag cagcattacg acaacttccg cgaccgtttc
    26281 gccaactggc cggtacgtat cgaaatgatc tcccgtttcc gcagcgccaa agagcagacg
    26341 caaatccttg cggaagtggc ggaagggaaa atcgatattc tgatcggtac gcacaaactg
    26401 ctgcaaagtg acgtcaagtt taaagattta ggcctgctga ttgtcgatga agaacaccgc
    26461 ttcggggtgc gtcataaaga gcgcattaaa gcgatgcgcg cgaacgtgga tattctgacg
    26521 cttactgcaa cgccgatccc acgcacgctg aatatggcaa tgagcggaat gcgtgacctg
    26581 tcgattatcg ccacgccgcc cgcccgtcgt ctggcagtta aaacctttgt ccgtgagtat
    26641 gacagcctgg tggtccggga ggcgatcctg cgtgaaattt tgcgcggggg gcaggtttat
    26701 tatctctaca atgatgtgga aaacatccag aaagctgccg aacggctggc agaactggtg
    26761 ccagaagcgc ggatcgccat cggtcacggg cagatgcgcg agcgcgaact ggaacgggtg
    26821 atgaatgatt tccatcatca acgtttcaac gtgctggttt gtacaaccat tatcgaaacc
    26881 gggatcgaca tcccgacagc caacaccatt atcattgaac gcgcggatca cttcggtctg
    26941 gcgcagctgc accagttacg cggtcgcgtc ggacgttcgc atcatcaggc atatgcatgg
    27001 ttgctgacac cgcatccaaa agcgatgact accgatgcac aaaaacgtct tgaagcgatt
    27061 gcctcgctgg aagatctcgg ggcaggtttt gcgctggcaa cgcacgatct ggagattcgc
    27121 ggcgcgggtg aactgcttgg cgaagaacaa agcggctcaa tggaaaccat cggtttctcg
    27181 ctgtatatgg agttgctgga aaacgccgtc gatgcactga aagccggacg cgagccgtcg
    27241 ctggaagatc tcaccagcca gcaaacagaa gtcgagctgc ggatgccgtc gctattgcca
    27301 gatgatttca ttcctgacgt gaatacgcgt ttgtcgttct ataaacgtat tgccagcgcc
    27361 aaaacggaaa acgaactgga agagatcaaa gtcgagctta tcgatcgctt cggcctgctg
    27421 ccagatccgg cgcgtaccct gctggatatt gcccgtctgc gccagcaagc gcagaaactg
    27481 gggatcagga agctggaagg taatgagaaa ggcggggtga tcgaatttgc cgagaagaat
    27541 cacgttaatc cggcctggtt gattggtttg ctgcaaaaac agccgcagca ttaccgcctt
    27601 gatggtccga cgcgcctgaa atttattcag gatttgagtg agcggaaaac gcgtatcgaa
    27661 tgggtacgcc agtttatgcg tgaactggaa gagaacgcga tcgcttaatg gtaaatcggg
    27721 ggcgtttctg cgcccccatc tgcaacattt acaaattctt tgcacttccc tgcactatcc
    27781 gacactgtca ccatccataa ttcaggctta tctgtttatt acaataacct tatatttatt
    27841 attgattttt ggtgatgatc aaaacgcgtt tttctcgctg gctaacgttt tttacgttcg
    27901 ccgctgccgt ggcgctaccg gcaaaagcca acacctagcc gctgccgcca gcgagcagtc
    27961 gtctggttgg cgaaaacaaa tttcatgtgg tggaaaatga cggtggttct ctggaagcca
    28021 tcgccaaaaa atataacgtc ggctttctcg ctctgttaca ggctaacccc ggcgttgatc
    28081 cttacgtacc gcgcgcgggc agcgtgttaa cgatcccgtt gcaaacccta cttccagacg
    28141 cgccgcgcga aggcattgtg atcaacattg cggagctgcg tctctattac tacccgccgg
    28201 gtaaaaattc ggtaaccgtg tatccaatag gtattggtca gttaggtggt gacacgctga
    28261 caccgacaat ggtgaccacc gtttcagaca aacgtgcaaa cccaacctgg acgccaacgg
    28321 caaacatccg cgcccgttat aaagcacagg gaattgagtt gcctacggta gtgccggctg
    28381 gaccggataa cccaatgggc catcatgcga ttcgtctggc ggcctatggc ggcgtttatt
    28441 tgcttcacgg tacgaacgcc gatttcggca ttggcatgcg ggtaagttct ggctgtattc
    28501 gtctgcggga tgacgatatc aaaacactct ttagccaggt caccccaggc accaaagtga
    28561 atatcatcaa cactccgata aaagtctctg ccgaaccaaa cggtgcgcgt ctggttgaag
    28621 tacatcagcc gctgtcagag aagattgatg acgatccgca gctgctgcca attacgctga
    28681 atagcgcaat gcaatcattt aaagatgcag cacaaactga cgctgaagtg atgcaacatg
    28741 tgatggatgt ccgttccggg atgccggtgg atgtccgccg tcatcaagtg agcccacaaa
    28801 ctctgtaaat cctggataaa aaaaatcccc gcgacacggc atgtcgcggg gaaaaaatca
    28861 gcagtagcag gcattaatga gggttaatgc ttatttataa attactgctg ttccatggag
    28921 ggtattcgga ccggttacag aagtaatacg gaaagatttt gcgcccatct catccgcttt
    28981 ttgcgccagc tgctcttcca gcgatcccag atttgtcccc gcgttagcac tgatagtacc
    29041 gactttttgt tggccttctg gcgttgactg aacttcgaca gccgcaaagt tggcaaatga
    29101 catggagctt aaaatcgccg cagcgatgag ggtttttacg tttttcataa tagtggcctt
    29161 atgcagatga atgacgtcgc aagaaattac ttaacgatcg ataactaaat gatagatgtg
    29221 atctggatca catacaagat attttttata acaatcatta attaatttaa aaatctttaa
    29281 atttcagtta aatagaaagc aattattttt atgggatctg gactggtgaa cggaggtgat
    29341 tatttttata ataacctttc attcacagtc accaggttca acggcatcat ggcaactgac
    29401 tcaacacaat gtgtaaaaaa aagccgtggc cgcccaaaag tgttcgacag ggatgccgcg
    29461 cttgataagg ccatgaaatt gttctggcaa cacggttatg aagcgacttc tcttgcggac
    29521 ctcgtcgaag cgaccggagc caaagcgccc acgctatacg cggaatttac caacaaagag
    29581 gggttatttc gtgccgttct cgaccgctat atcgatcgtt ttgccgctaa gcatgaagca
    29641 cagctgtttt gtgaagagaa aagcgtggag tctgcgctgg ctgactattt tgctgccatc
    29701 gccaactgct ttaccagcaa agacaccccg gcaggctgct tcatgatcaa caactgcacc
    29761 accctctccc cagattcagg agatatcgcc aatacgttga aatcacgcca tgcgatgcaa
    29821 gagcgcactt tgcagcagtt tttatgtcaa cgacaagcgc gcggggaaat cccgacccac
    29881 tgtgacgtga cacatctggc agaattcctt aattgtatta ttcaggggat gtcgatcagc
    29941 gcacgcgaag gtgcatcgct ggaaaaactg atgcagattg ccggaacgac tttgcgttta
    30001 tggcccgaac tggtgaaata agtcgcaaaa aaagcccctc agcctgagcg aatgaggggc
    30061 caaattgcag agtacagcct tgttatactt tgttattcaa aatcagttgc ccgttactat
    30121 ctagcgggat ctgcgtaccc ggatcgcggt ccatgcggat tttgccctgc tgatcgccaa
    30181 tcttataggt cacatcataa ccgagcattt tttctgactt gtcatacacc gttttacaac
    30241 gctgttgcgt agtcgtgtaa gtatcgcttt cctggagaga gccctggatc tggttaccgg
    30301 catatccacc acccagcgcc cccacaacag tggcgacatc tttaccacga ccaccaccaa
    30361 actgatgccc tatcacgccg ccagcaacag cgccgagcac cgacccggta atgcgatttt
    30421 catcctgcac cggtcgacga tgggtcactg tgacgttgcg acactcctga cgcggtgttt
    30481 taaccgtttc cttgattggg gttgcagaaa caacctgagc gtattgcggg ccccgttcaa
    30541 acacgttcag actggccact gccgctacgc ccagcgcagc tgcgacacca atcccgatac
    30601 ccgccaacat tgatttattc acgggaacac ctccttcttt gcagttatgc caatcatgca
    30661 agcgaaggag aaaagcagct atcagacaga agatgaaaag cgcgggaatg gctggaaata
    30721 cttaaggatt cggagaagtc tgaaggaatg cctgatgcga cgcttaacgc gtcttatcag
    30781 gcctaccgct cgtaccattt gtccagggcg gatgaggcgt ttatgccaca tccgccagtg
    30841 tacgtcgatt aatgcaactt caaacgcgga cggataacgc ggttaatact ccccaccagc
    30901 atcattaatc cggttttaaa gtaaccatgc agcgcaatct gatgcattcg gtatagcgag
    30961 atatatacaa agcgcgcaat tcgtccttca atcatcattg agccgcgcgt caggttaccc
    31021 atcaggctac cgacagtgga gaagttcgac agcgatacca gcgaaccatg atctttatac
    31081 tgataatttt tcagcggttt accgttcatc tgcgccagaa tatttttcat tgcgcaggtc
    31141 gccatctggt gtgcagcctg agcacgcggc ggaacaaagc ccccttccgg acgcgggcat
    31201 gacgcgcagt cgccaatagc gtaaatgtct ggatcgcggg tggtttgcag cgtcggttcc
    31261 accaccagct ggttgatacg gttggtttca agaccaccga tatctttcag gaagtctggc
    31321 gctttgatcc cggctgccca caccatcaga tcagcctcaa tatattcacc atctttagtg
    31381 tgcaggccgc cttcatcagc actggtgacc atggtttgcg tcagcacgcg aacgccaagt
    31441 ttcgttagct cgttgtgggc cgcagcagag atacgtggcg gtaatgcagg caaaatacgt
    31501 tctcccgctt ctaccagcgt tacgttcagg gcttcgttgg tcaggccttt gtaaccgtag
    31561 ctgtgcagtt gcttgaccgc attgtacaat tcagcggaga gttctacacc cgtcgcgccg
    31621 ccgccgacaa tcgcaatgtt cactttacca ttcgcgccca ggttggcgga gtatttcagg
    31681 aacagattca gcatctcctg gtggaagcga cgcgcctggt gcgggttatc gaggaaaatg
    31741 cagttctctt tgacacctgg cgtattgaaa tcgttagagg tgctacccag cgccattacc
    31801 agggtgtcat aggcgatttt acgttccgga accagcagtt cacctttctc atcgcgcagt
    31861 tctgcaatag tgattgtttt cgcttcacga tcaatatcaa tgacggaacc cagctggaac
    31921 tggaaaccat gattgcgcgc atgggccaga tagctcaacg catcgacgcc ttcatcaagc
    31981 gagccagtcg ccacttcgtg cagcagcggt ttccacagat ggctgtggtt acgatcgacc
    32041 agcgtaattt tggctttttt cttgcgtccc agcttatgcc ccagctgtgt tgccatttcc
    32101 agcccaccag caccgccgcc gacaatcaca atttttttca atggcgtagt caacgtgacc
    32161 cccttaaatt tattaaccaa ttgttaatta atggttataa acatagctct taattaacaa
    32221 caggttacag caatgaaaat gttcttcgct attgagaata acatgaatgg tgcattggtc
    32281 ataccaaaat tgatgttaat caagttttgt tgctgaaaag ataggcgact ttaccgggtg
    32341 agatcaaaaa acagagttta ttgtttatta tttgagcaag tccggaaggg tgatataggg
    32401 tgacgcgtat atcgagggcg tttggtcgtc ctgataagat gcgcagcatc gcatcaggca
    32461 ttgatgctcg actgtcggat gcggcgtaaa cgccttatcc gacttacaga attcggagcg
    32521 catcaggtac cggcaaggtt acctgatgcg acgagggcat ttacccgagg gttttgaacg
    32581 ccttaatgcg ctgcaaatgc ggggagatat tcttgaattt gtgcgtctgc tcttcgtccc
    32641 agacaatctc gtaataatga tgcaactctt cagatgtccg ctggctgtta agcgcttcat
    32701 cattacgcga caaaatcacc aggcaacgat cgcgattctt ctcgcggaag ttggtcacac
    32761 acttagtggc aatatcggca tactcttccg ggcgatcaat cttgccttcc atgttctcat
    32821 aagggaacaa attagggttg aagatcacct ggcggatatc gcaaagaaaa ccaatccgtt
    32881 ccgcccagta tccgcccaga ccaacgccgc aaatcagcgg acgctcgtcg acgttcagtt
    32941 gcaacatttt gtccacttct ttgagcagat gctgcatatc atgtttcgga tgccgcgtac
    33001 tgtagcttat caagcgtaca tccgggtcaa taaactgcaa ttgtaagact ttctcgtggt
    33061 tacccggact gttagagtca aaaccgtgta aatagataat catcgcttcc tcacataagc
    33121 cagggttagt gacctgcttt ctcttcctgc cagcgttcat gtaactgatt cagacgggtg
    33181 ctgatcgcct tccagcgagc cgagtccatc agttcctgtc gcgaaaatga acctttatga
    33241 tacaaacgtg taacacgttc tgccttgatc ggtgacagat tatctaacac gctgacggcc
    33301 cctttacgat tattgcagac caggatcata tcgcaacctg cgtccagtga tgcctgcccg
    33361 cgttcggcat aactgcccat aatcgcggca ccttccatcg ataaatcgtc agagaaaatt
    33421 acaccgtcaa aacacagttc ctgacgcaaa acggttttca gccagtagga agaaccgctc
    33481 gccggacgcg gatcaacatc actgtagatc acatgcgcag gcataatggc gtcgagttta
    33541 ttttcgcgga ttaaggaact gaagaccgac atatctttag cgcgaatctc cgcttgtgga
    33601 cgtggatcgc acggtgtttc tttgtgtgag tctgccgtta ctgcaccgtg tcctgggaag
    33661 tgtttcccgg tcgttttcat tccggcttca tgcataccat caataaaccg gctggcaatt
    33721 gccagggctt tttgtggatc ggcatgataa gaacgctcgc caatcgccgc gctgatatgc
    33781 ccgacatcca gcactggcgc aaagctgata tcaatatcca tagcgatcat ttcgctggcc
    33841 atcagccaac ccgcctcttg cgccagtttg ccgccctctt ccattcccaa cagcgcagca
    33901 aaggattgtg ctgccggtaa gcgggtaaaa ccttcgcgaa aacgctgcac gcgtccacct
    33961 tcctgatcta ccgccaccac cagatgattg cgcgatgctg cgcggatctg gcgcaccagt
    34021 tcacgtaact gggcaggatc atgatagtta cgcgtaaaga gaatcagccc tcccaccagc
    34081 ggatgcgcca gtatttcacg ctcttccgcg tccagttcgt aaccttcgac atccaacatt
    34141 actggaccca cactgctctc cttattgttt tattaatagc tgccgccagg tgtcatcggc
    34201 cagcctgata aattgttgat cgccgatttg tcgccagcgg tactcaaacc accctgcttt
    34261 gagcatcagc agccagggaa accatcgcct gacctgacgc cataattgcg ccggataaat
    34321 cttcgcgcga gtggcatagt cattgaccaa ttgccggtgc tgttcagtat tttccaccca
    34381 caccgccgcc agttccagcg cgatatcacc atctccggca tactcccagt cgatgagttt
    34441 taaccctgac gcgctatgca ctaaatttcc ggcgtggaca tccatatgca atggacttaa
    34501 gcgtaaaggc cgtggttccc gcgctttgcg cagacgtttt aacattcgca gccaacccac
    34561 agtccgccgc gccggatcgc tttgctgcca gtacagttcc agtaagggca acagcgttat
    34621 tcgccagcca aaacgtggtt gttgatgaag ataatacagc aagcctgcca gttcgttggt
    34681 atccggcaaa tacgttttta ccgcgccggg cagatagtcg actaccatcc agtcacggag
    34741 atataaatgc ggcttcggtg caatgcttgc gggtagttgt gataaagccc gatactggcg
    34801 caagaacgcg gactgcggcg catcaggatc gtgcggctga cgcacaacaa aacgctggcc
    34861 ctgatgttca atgagaaaac taccgccact aagcccacta ttaaacgtcg tgacgggatg
    34921 aaactgcggg aaaaagcgcg acagcaattc gtcgcgcgtg atgggattat tgctgcgaaa
    34981 cggcaccttt acctgaccag ataatttcgc ccgtctgcac cagcatcagc tgcatttgta
    35041 gggtcggagc cttaacgttg ccagaggcgc tggagtacag cacgtaatga gcgccgacat
    35101 tgcgggcaat gcctatggct ttactacggg tacctaaact gtcctgcggc gacaaaccta
    35161 actgttgctt agccatcgac agctgctggg cggaaaccag ggtaaatttc ccgttattag
    35221 ccagcgcatt tcgcagcgtt tcggtcgctt ctgcggcatt cagcgaaccg ttagtacggt
    35281 tgttaacgct atcaaccagc aggacgctac ccgcagtcac cccgtcagcc ccaagcatct
    35341 tactgaccat cggctgcatt gcgccattcc agtcataatg gcgaatatgc ggcgcaggcg
    35401 gtgccgtttg atcttcgtgc tcaattgggc ctggctgctg cgggatcgtc ggcaccgagg
    35461 gcactgtggg gacaggctgt tgtggctcgg ctggttgttc cggcgctggt ttcacttctt
    35521 ctaccggtgc aggttcacgt tgccccacac acccggcgag aaacatcgcc agcgcggtaa
    35581 tcaaggcgta gcgactcatt tttgtcatca agattcaccc cttacaaata tagataaagt
    35641 ctgactttgt gcgcccccag aaaattggcg ctgccgtaca gcgttaccgc cgaatgtgcg
    35701 ggaatgctaa cgctgcgtgg cctttccaga ggatgcatct ccagccctct ggcgtcatac
    35761 cagtaaaaac gataatgaac ggtaacgggt tcttgccttt cgttataaag cgttgaggat
    35821 gctgaaggtt gaatatcaga cgtcgaaagg acgggctttt ctgcactgat gcccgcagcc
    35881 aataaagatg actccatcac cagcgattgc tcatcattca ccggaatttc cggatgtgaa
    35941 cgacagccca ccagcagtaa caacgccaga gacaccagcc caaagcatcc ttttctcatc
    36001 gttacagacc tttatgcgcc agcattggtc ccagcggacg gccacccaac aagtgcatat
    36061 ggatgtggta aacctcttgt ccgccatggc ggttggtatt catgatcaga cgatagccat
    36121 cttcggcaat accttcttgc tcagcaattt ttgccgctac ggtgatcatg cgtcccagcg
    36181 cctgctcatg ctcagctgac acgtcgttca cagtcggaat gaggatattc ggaatgatca
    36241 gaatatgcgt tggcgcttgc ggcgaaatat cgcgaaacgc cgttaccaga tcatcctggt
    36301 agacgatatc ggaggggatc tcacgacgaa taattttgct gaatatagtt tcttctgcca
    36361 cgacattttc ctttttcaaa tagccatgcg ttgcgccatg ctacgccgcg ccacactgcg
    36421 agtatagagt atggtcgagt taaacctgct ctttcaacct taactcacga tttcttaagc
    36481 aaaaaatcgt tttgctgccg tatatatcgc cattattccc atttctgcct gcgaatgcgc
    36541 tgaaaagaca ttctctgttt ggtttacacg acaaatatga atgcgcatat ttctcatttg
    36601 catttacaaa caaaattatt cgcacactaa atataaccgc tcatcaccct cctccggcat
    36661 gagcctgacg acaacataaa ccaagagatt tcaaatgctt tcaacacaat ttaacaggga
    36721 taatcaatat caagccatca ccaaaccgtc actacttgcc ggttgcatag cactggcact
    36781 attaccttct gccgcttttg ctgcaccagc cactgaagaa acggtgattg ttgagggttc
    36841 agccacagct ccagatgatg gcgaaaatga ttacagcgta acgtctacct ctgcgggtac
    36901 caaaatgcag atgactcaac ttgatattcc tcagtcggtc actattgtta gccagcagcg
    36961 gatggaagat cagcagttac aaacgctggg cgaagtgatg gaaaacacgc tggggatcag
    37021 caaaagtcag gcggattccg atcgtgctct ttattattcc cgtggattcc agatcgataa
    37081 ctatatggtt gatggtatcc ccacctattt tgaatcgcgc tggaatctgg gcgacgcact
    37141 ttctgatatg gcactctttg aacgcgtaga agtagtgcgt ggcgcgacag gactcatgac
    37201 cgggacgggt aatccatctg cggcaattaa tatggttcga aaacacgcga ccagtcgtga
    37261 atttaaaggc gatgtctcgg cggaatacgg tagctggaac aaagaacggt atgtggcgga
    37321 tttacaaagc ccactcaccg aagacggtaa aatccgcgcg cgaattgtcg gcggctacca
    37381 gaataacgac tcatggctgg accgctacaa cagtgaaaag accttcttct cgggcattgt
    37441 cgatgctgat ttaggcgatc ttacgacgct ttcagccggt tacgaatatc agcgcattga
    37501 tgttaatagc ccaacctggg gcggtttacc gcgctggaat actgatggca gcagcaacag
    37561 ttacgatcgt gcacgcagta ccgcacctga ctgggcgtac aacgataaag agatcaacaa
    37621 ggtctttatg accctgaagc agcggtttgc tgatacctgg caagcgacac tgaatgccac
    37681 ccactctgaa gtcgaatttg acagcaaaat gatgtatgtc gatgcctatg taaacaaagc
    37741 ggatggtatg ctggttgggc catacagtaa ttatggacct ggctttgatt atgtcggcgg
    37801 caccggttgg aacagtggca aacgtaaagt tgatgcgctg gatttgttcg ctgacggtag
    37861 ttatgaattg tttggtcgtc agcacaatct aatgtttggt ggcagttaca gcaaacaaaa
    37921 caatcgttac ttcagttcat gggccaacat cttcccggat gaaattggca gtttctacaa
    37981 ctttaatggc aatttcccac aaaccgactg gtcaccacag agcctggcac aggacgatac
    38041 cacacatatg aaatcgttat atgctgctac tcgagtcacc cttgccgatc cgctgcatct
    38101 gatcctcggc gcacgttata ccaactggcg ggttgatacg ctgacttaca gcatggagaa
    38161 aaaccacacc acgccttacg ctggtctggt gtttgacatc aatgacaact ggtcgaccta
    38221 cgccagctat acctctattt tccagccgca aaatgatcgt gacagttcag gcaaatatct
    38281 ggctccaatc accggtaaca actacgagct gggtctgaaa tcggactgga tgaatagccg
    38341 tctgaccacc acgttagcca tcttccgtat tgagcaggat aatgtcgctc agtccaccgg
    38401 tacacctatc cccggcagca acggcgaaac cgcctataaa gcggtggatg ggacagtcag
    38461 taaaggggtg gaatttgaac tcaacggcgc aattaccgac aactggcagc tgacatttgg
    38521 cgcaacgcgc tatattgcag aggataacga aggaaacgcc gttaatccta atctgccacg
    38581 caccacggtt aaaatgttca ccagctatcg gttgcctgtc atgccagagt tgacagtcgg
    38641 cggtggtgtt aactggcaaa atcgcgtgta taccgacacc gtgacaccgt atggcacctt
    38701 ccgcgccgag caaggtagct acgcgctggt ggatctcttc acccgctacc aggtgacgaa
    38761 aaacttctcg ttacagggga acgtcaataa cctgttcgac aaaacctacg ataccaacat
    38821 ggaaggttct atcgtctacg gcgcaccgcg taatttcagc attaccggca cgtatcaatt
    38881 ctgaataaca cctgtaaaaa aggcagccat ctggcggcct tagtctcccc aacgtcttac
    38941 ggattagtgg ttacggatgt actcatccat ctcggttttc aggttatcgg atttagtacc
    39001 gaaaatcgcc tgaacaccag aaccagcaac cactacgccc gctgcgccca gtttcttcag
    39061 gccagcctga tccactttag acacatcagc aacgctgacg cgcagacggg taatacatgc
    39121 gtcgaggtta gtaatgtttt ctttaccacc aaatgcagca accagagccg gtgccatttc
    39181 gctggtacct gttgcttttg catcttcagt cgcgtcttca cgacccggcg ttttcagatc
    39241 cagtgcttta atcagcacgc ggaagatggt gtagtaaaca atcgcataac cgataccgac
    39301 gatcgggaac agccacagtt tgctgctgtt accagacaga acgataaagt cgatcagacc
    39361 gtgcgagaac gacgtaccgt cacgcatccc cagaagaata cagattggga atgccaggcc
    39421 tgccagaatc gcgtggatga tgtacaggat cggcgcaacg aacatgaagg agaactcgat
    39481 cggctcggtg ataccggtca ggaacgaggt cagcgccgcg gagatcataa taccgcccac
    39541 tttcgcgcgg ttttctggtt tagcagagtg ccagatagca attgcggcag ctggcagacc
    39601 gtacattttg aacaggaagc caccagacag tttacccgca gtcgggtcac ccgccatata
    39661 acgcggaatg tcgccgtgga aaacctgacc tgctgcgttg gtgtattcac caatctgcat
    39721 ctggaaaggt acgttccaga tgtggtgcag accaaacggc accaggcaac gttcgatgaa
    39781 accgtaaatg ccaaacgcaa ctaccgggtt ctggtaagca gcccactgag agaaggtctg
    39841 gattgcagaa ccaatcggcg gccaaatgaa ggacagcaca acgccagtaa agatggcagc
    39901 caggccagaa atgatcggca caaagcgttt accggcaaag aagccaagat actcaggcag
    39961 cttaatacgg tagaaacggt taaacatgta cgctgcgatc gcaccggaga taatccctcc
    40021 gagtacgcca gtatccgcca ggtgtttaga ggcgatttct tcagcaggta aatgcagtac
    40081 cagtggcgca accacggcca tggttttaac catgatgcca taggcaacaa ctgcggccag
    40141 cgcggatacg ccatcgttat tggtaaagcc gagggcgaca ccgatcgcaa aaatcagtgg
    40201 catgtttgca aagacggaac cgcctgcttc tgccataaca tgcgatacaa cggcgggcag
    40261 ccagctgaaa ttcgcggaac cgacgcccag cagaatacct gcgataggca gtacggatac
    40321 cggcagcatc agcgatttac cgaccttttg caggttagca aatgcattct taaacataat
    40381 tgagagtgct cctgagtatg ggtgcttttt ttacgttctc acgcgtggca aggggggaga
    40441 gcctcgccgt gtacagggca tctaagcgcc ctttatttat tacacagagt aaaataattc
    40501 agtgccgatt tgtttgacgg ctatcacatt tcaacttcag atcattggag aagttgcaca
    40561 ttttgacaaa acatatgtca caatatttca aacgtggtta ttcaccgccc aattttcggc
    40621 ggtgaacttt actcgtttta gccattaatt acgagcttta aaaaaactca ttcaacggat
    40681 ggattgaagg cgggaagcgt cgatgtgaaa cagacgggcg aagttatcgg tggttacctg
    40741 cgccagttct tcaacggcaa cacctttcaa cacagccatg tattctgcaa cgtcacgaac
    40801 catcgcaggt tgattctctt ttcctcgatg cggtaccggc gcaaggtaag gtgagtcagt
    40861 ttccaccagt aaccgatcca gggggacata acgcgcagca tctcgaagtt gctccgcatt
    40921 acggaaggtc acaatgccgg aaaaggagat gtaaaatccg agatccagta atttacccgc
    40981 cgtttctctg tcctctgtaa aacagtgtag tacgccaccg caatccgtca ctttttcttc
    41041 gcgcagaatt gccagcgtat cggcgcgggc gtcacgggta tggacgataa ccggcttgtt
    41101 cagttcacga ccaatctgga tatgatggat gaaggactct tgctgacgaa ctttagtttc
    41161 cggtgtgtaa taataatcca gcccggtttc acccagcgct acaacaccct cttctgccgc
    41221 cagacggcgt aaatcttcta catcgtaggg atcattctgg ttgagtggat gcacgccaca
    41281 agaaaatacg acgttgtcac gttcgcctac cagatcccgc atatgtaaat aacccggtaa
    41341 tgttgtggcg actgccagac aaaatttcac atcgcgtgcg gcggctttcg ccagaacgtc
    41401 atccacgtcc ttatgcaaag attcataatc cagaccatcg agatggcagt gtgagtcgac
    41461 taaaaacatg atgtctctct taaagatgag gaaccggtag cacaacgccc ggttgcaggt
    41521 aatgctcaat acgcagtaaa agatcggtga tgagaagctc gcggttgatg cctgtgacag
    41581 acattaactg ttcacgaatg tggcaaacat cccccagtat agcctgcagg cgcgagggag
    41641 aaagatggtt tgccagttcg acgacaaggc ccggcacatc aacattggtc acctgcgcag
    41701 caccatgatg gcgttttagc gcatccatca gcaacgttgc cagccagtgt aaacgcgccg
    41761 gagcttgttc atgattaagg gccgccagca gcgaatacca gtcgcccgat ggcacgctat
    41821 atgccaatgc ctgacacaat gtttcacgag cctgccagtt atctccctga aacaacgcca
    41881 gtgccgcgcc aggcgaaccg gcgcttaagc gcaatgcggc aagtaatgca tcctgtgaca
    41941 ttgtcacttc gcgtgaaagc caggtcacgg cgtactgttc cggcggcggc gcaaggtaat
    42001 gtaaccgaca acgactacgt aatgttgcca gtaaacgttc aggctcgcgg gtagccagga
    42061 aaaaccaggt ttctgctggt ggctcttcaa gcgttttcag caatgcgtta gccgcggcgt
    42121 cggtcagtaa ggcagcatcg gttacccaga caactttcgc accacctagg cgtgcgtgct
    42181 cattcagctt ttcggtgacc tcacgtactg catcaatgcc cagcgtattt ttcccttttt
    42241 cgggagccag ggtgtagtaa tcgggatgcg tgccagcctg catcaactga catccacgac
    42301 agtgaccgca acttttgtgg ccctgcggtt gttggcagag taaataacgg ctcagggcgt
    42361 agattaaagc atcatcgccc atgcccggta acgcctgaat gagtagcgca tggtgacctc
    42421 ttccggcctg atagctggct accagttttt cgaaatcagg tcgtaaccat ggataccatc
    42481 tcatgcgtcc aactccttca cccagtgggt cacggtagtg cggattgcat ccatcacggc
    42541 ctccagcggc tgggtggcat caatggtatg aatactttta tcttgtgcag ccagttccag
    42601 atagcgggcg cgggtgcgat taaagaaatc gaaagactct tgctcaatac gatccagctc
    42661 gccgcgcgca cgcgcgcgtt ttaagccaac ttccggggta acatcgagat agagcgttaa
    42721 gtcggggcga aaatccccga gaacagcatc acgcagtgtt gccagcatat gttggtcaat
    42781 accacgtcct ccgccctgat acgcctgagt ggagagatcg tggcgatcgc caatcaccca
    42841 ggtgccgtta gccagagctg gtttgatgac cgtttctacc agttgaacgc gcgcggcata
    42901 aaacatcaga acttcggctt tatcggtaat gacttcatcg cctaccgatt tgatatccag
    42961 caccaggctt cttaactttt cggcaagttg cgtaccgcca ggttcccgag tgaaaaccat
    43021 gtcgcggata cccagttgct caagcgtctc aaccaccaca ttacgcgcgg tagttttgcc
    43081 tgcgccttcc agcccctcaa tgacgatata cttactgcgc atttttttcc ttaagcactt
    43141 tcagataatc ctgcacagac ttgttatggc tggcaagatt ggtattaaac gtgtgaccac
    43201 ctttaccatc ggccacaaaa tagagatacg gcgtttttgc cggatgcgca gcagccttca
    43261 gcgaatccgc ccccggcgta gctatcgcac ccggcggcaa accggtaatg gtataggtgt
    43321 tatacgctgt cggcgtttcc aggtctgcac gagaaagttt gccattataa cgctctccca
    43381 tcccgtaaat cacggttggg tcggtctgca ggcgcatacc aatgcgtaaa cggttgataa
    43441 ataccgaggc aacctgatcg cgttcactgg caacggcggt ttctttttcg ataattgatg
    43501 ccatcgtcac cagctgattt ttatctttat aaggcagacc gtccgcacgc ccttcccagg
    43561 cgctatcgac cgctttcacc attttcttgt gcgctcgctt gagtaacgcg acatcggtgg
    43621 tattggcggt atacatccag gtgtctggcc agaaccaacc ttcaatccac tccgggtttt
    43681 ccagttcaag tgcctgcgct acggtggcgt acttatcgtc actcagcgtg tgcttgatat
    43741 acggagcctc acgcaattgc ttgaggtagt cgctcagacg catcccttct accagtcgca
    43801 gcgggaactg tgcttcttta ccgctttcca gcaatttcag catctcgcgc acggtcatct
    43861 gcggtgtaaa gcggtaagtc ccggctttaa agtgagaaag atccggttcg atacgcagca
    43921 accattgaaa aacccgtggg cgattgatga tcttatcggc ataaagctgt tcaccaagcg
    43981 ccagacgtcc ggtccctggc ttcagggtaa atatcgtctc ttctttgata agcaatttgc
    44041 tgtcggcaag atggcgaacc ttccagacgc ccacaccagc ggcgataccc agtaccacca
    44101 gcaataacaa gataatcaat aacacttttt tcatgactaa ttcgggcgct cacaaagtgg
    44161 ggctaaatat tcatataacg ttgctgacga aaaggagaca tcgccacagg cacgtacggg
    44221 catcactggc attaacgcat tacaaataac catctcatct gcctgcaacg cctcttccag
    44281 agaggcttgc acttcgacaa gctgataaga ggattgtgcc agcaaacgga tacagaattg
    44341 tcgcataatg ccgttaacac ctgcctgatc cagacgcggc gtataaacta cattgccctt
    44401 ccgccagaac aaattagccg cacagcattc cgtaacccac ccttcgctgt caaggaccag
    44461 cgcctcatca gcgtttgtct gctcaagatg agagcggatc aatacttgct caagccgatt
    44521 aagatgttta atacctgcaa gatgaggatt gcgccccagc cgcaccgggc ttagcgccaa
    44581 cgtcatccct tcgttacgca aacggtcgta atgtgcagga taaaccgtaa cggagagaat
    44641 ccgcgttgcc ggtccactgt tcaatgtgct gtaccctcgc ccgccactac cgcgactgat
    44701 cacgactttc agcacaccat tttgctgttc tgctgccagc gttttcatct cttgttcaag
    44761 ctgaggccag aagtcacaga aaatcatcaa ccgctgacaa gcatcctgta gtcgctggat
    44821 atgcgccgat aacaaactga ctttaccgtc gataactctg gcggtggtaa aacaaccatc
    44881 accaaactgc gttgcccgat cgcttactgc cagcgattcc tgcttatgac cgttaattaa
    44941 gaacataccg gctccttatc agagaattgt tagtgtggca gcatgttcac tacggaacaa
    45001 gtcggaataa aagctaagaa aaaaggcccg caagcggacc ttttatatgg gtgggaaatg
    45061 acaacttaga tctttttaaa gatcaaagaa ccattagtgc caccgaagcc gaaggagtta
    45121 cacagcgtgt attccattcc gctgacctga cgtgcttcat gcggtacgaa atccagatcg
    45181 caaccttcat ccgggttatc caggttgatg gttggcggaa cagcctgatc gcgcagcgcc
    45241 aggatggagt agatagattc tactgcaccc gccgcaccta acaggtgacc ggtcatagat
    45301 ttcgtggagc ttaccaacac acggcttgca gcttcaccga agatggtttt caccgcctgc
    45361 gcttcagctt tatcgccagc cggcgtagaa gtaccgtgcg cgttaacgta gccaatctga
    45421 ctcgcttcaa tgcctgcatc acgcagagca tttgccatcg ccagagctgc gcctgcgcca
    45481 ttttctggcg gtgacgtcat atgataagca tcgctgctca taccaaagcc gacgagttca
    45541 gcgtaaattt tcgcaccgcg ttttttcgcg tgttcgtact cttcaagtac cagcataccg
    45601 gcaccatcgc ccagtacgaa gccatcacgc tctttatccc acgggcggct cgccgcttgc
    45661 gggttatcat tgcgggtaga taatgcacgt gccgcgccaa aaccaccaac acccagcggc
    45721 gtactggctt tctctgcgcc acctgcaacc atcacgtcag catcgccata cgcgataata
    45781 cgcgcagcat ggccaatgtt gtgcacgccg gaagtacagg cggtcgcgat agagatgctt
    45841 gggccacgca ggccatacat gatagtcaga tgacctgcca ccatgttcac aatcgttgac
    45901 ggaacgaaga atgggctgat cttacgtgga ccaccgttca tcagagatgt gtggttttct
    45961 tcgatcagtc cgaggccgcc aatcccggag ccaattgcgg caccaatgcg ggttgcgttc
    46021 tcttccgtta tatcaaggcc agaatcctgc atggcctgaa cgccagcgac aattccatat
    46081 tgaatgaagg catccatctt gcgctgttct ttgcgcgaga taatgtcctc acagttaaaa
    46141 tcctttacta agccagcaaa tttcgttgca taggcgctag tatcgaaatg gtcgattagg
    46201 ctgatgccac tctgaccggc aagcagagct ttccaggtag actctacggt attgccgaca
    46261 ggagacaaca tgcccagtcc ggtcacaact acacgacgct tagacacgtt tgtcctccag
    46321 ggagggaaaa aatgattcta gtgggacaaa aagataaaac tcaggcggtc gaacgaccgc
    46381 ctggagatgt tcacttacgc ctggtggccg ttgatgtaat caatggcagc ctgaacggtg
    46441 gtgattttct cagcttcttc gtccggaatc tcagtatcaa actcttcttc cagagccatt
    46501 accagctcaa cggtgtcaag agaatccgcg cccaggtctt caacgaaaga agcattgttg
    46561 gtaacttctt cctgcttaac gcccagctgt tcgccgataa ttttcttaac gcgttcttcg
    46621 atagtgctca tactcttaaa tttcctatca aaactcgctt tcgcgatggt tttcgtagtg
    46681 tataaaatgt tgaaaaattt gcaactaaat cccggcaggt cttaccacga ttttacgtta
    46741 ttttgaggcc ttacccccca ataacgcaaa tcattttgca ctaattatta ctcataacca
    46801 cgcagactgc gcgcaacatg agcattttgt gcaaatcgcg gtcagaccat gtacatcccg
    46861 ccgttcacat gcaaagtttc acccgtgatg taagctgctt cgtcggatgc caggaatgca
    46921 accgcgttgg cgatttcctg tgcgccgccg aggcgacccg caggaacctg cgccaggata
    46981 cccgcacgct ggtcatcgct cagcgcacgt gtcatgtccg tttcaataaa gcccggagca
    47041 acaacgttta cagtaatacc gcgtgacgca acttcgcgcg ccagtgattt actgaagccg
    47101 atcaagcccg ctttcgccgc agcgtagttg gcctgaccgc catttcccat ggtaccaacc
    47161 acagaaccga tagtgataat acgaccatga cgctttttca tcatagcgcg cattaccgct
    47221 tttgacagac ggaaaacaga tgaaaggttg gtttcgataa tatcgttcca ctcttcatct
    47281 ttcattcgca ttaacaggtt atcacgagtg ataccggcat tattgaccag gatatccact
    47341 tcaccaaatt ctgcgcgaat tttttccaga acagattcga tagatgccgg gtcggtcaca
    47401 ttcaacatca gacctttgcc gttggcacct aaataatcgc tgatcgcctg agcgccattt
    47461 tcactggtcg cagtgccaat aactttcgcg ccacgggctg cgagcgtttc agcaattgcg
    47521 cggccaattc cgcggcttgc accggttacc agtgcgattt ttccttcaaa attcatgatt
    47581 ttcctctttt aaagctcaag cgccgctgcc atcgctgaag gttcgttcag cgccgaggcg
    47641 gtcagggtgt cgacaatgcg tttcgtcagg ccagtaagca ccttgcccgg gccgacttca
    47701 tagagatgtt ctacgccttg cgctgccatg tactcaacag acttcgtcca ctgaaccggg
    47761 ttatacaact gacgtaccag tgcgtcacgg atggcatcac cattggtttc gcatttcaca
    47821 tcaacgttat tcacaacaga aactgttggt gcgttaaagg tgattttcgc taattctact
    47881 gccagtttgt cggctgctgg tttcatcagc gcacagtgag acggtacgct cactggtaac
    47941 ggcagagcac gttttgcgcc cgcagcttta caggcagcgc cagcacgctc aaccgcttct
    48001 ttatgaccgg caataaccac ctgtcccgga gagttaaagt ttaccggaga aacgacctga
    48061 cctgctgcag cttcttcaca cgctttcgca atagacgcat catccagacc gatgattgcc
    48121 gccatagcgc ccgtaccttc cggtacggct tcttgcatga gcttgccgcg catctcaacc
    48181 agacgcaccg catcagcgaa atcaatcaca ccagcgcaaa ccagcgcgga atattccccc
    48241 aggctgtgac cggccatcat tgccggtgct ttaccgccct gctgctgcca tacgcggtac
    48301 agcgcaacag aggcagtcaa cagcgctggc tgggtttgcc aggttttatt cagttcttca
    48361 gctggcccct gctgggtcag cgcccacagg tcgtagccca gcgccgcaga agcttcagca
    48421 aacgtttctt cgacaattgg atagctcgcc gccatatcag ccagcattcc aacggtttga
    48481 gaaccctgtc cagggaacac aaatgcaaat tgcgtcatgt tttaatcctt atcctagaaa
    48541 cgaaccagcg cggagcccca ggtgaatcca ccgccaaagg cttcaagcag aaccaactgc
    48601 cccggcttaa tgcgcccgtc gcgtacagct tcatccagcg cgcacgggac agaggccgca
    48661 gaggtattac cgtggcgatc cagcgtcacc acgacattgt ccatagacat accgagtttt
    48721 ttcgccgttg cactgataat acgcaggtta gcctgatgcg gaaccagcca gtccagttga
    48781 gaacggtcaa gattatttgc cgccagcgtc tcatcaacga tgtgcgccag ttccgttacc
    48841 gcaaccttga agacttcgtt gcccgccatc gtcagatgaa ttgaattctc tggattcaca
    48901 cggtcagcat taggcagcgt cagcaactca ccatagctac cgtcggcatg cagatgggtg
    48961 gagatgattc ccggctcttc agaggcagcc agcaccgcag cgcccgcgcc atcgccaaaa
    49021 ataataatag tcccacgatc ggttggatcg caggtgcgcg ccagtacatc ggaaccgacg
    49081 accagagcat acttcaccgc cccagatttc acgtattgat cggctacgct taatgcatag
    49141 gtgaaacctg cgcaggctgc tgcaacgtca aatgccgggc aacctttaat gcccagcatg
    49201 ctttgaatct gacaagctgc gctcgggaaa gcgtgcgtag cagaagtcgt tgccacaacg
    49261 atcaggccaa tctggtcttt ctcaatgccc gccatctcaa ttgcgcgtgt cgccgcttca
    49321 aagcccatgg ttgaaacggt ttcgtttggc gcggcaatgt ggcgttcgcg gataccggta
    49381 cgagtgacaa tccactcgtc agaggtgtcc accatttttt ccaaatcggc gtttgtccgc
    49441 acttgttcgg gcagatagct gccagtacca ataatcttcg tatacatgta cgctcagtca
    49501 cttttcggtt atataccgtc acttgcaaac tgcgagttcg ctggcagcgt cctgctaccg
    49561 cagagttccg cttttgccac cgtccagcag ctcaaaacca gctgggtata cagattccag
    49621 gcgagcggca attcgctgag gaacttgtcg ctgcaccgcc tgcactgcct gttcaatcgc
    49681 gaccgcaaaa gctcgctgat tggctgcacc atgacttttt atcaccgtgc cgcgcaatcc
    49741 taacagacag gcgccgttat actggtcggg gttgaggtga ctgaatcgcc tcgtcaggct
    49801 cttttgtagc caacgcttta ataacagtag ccaccacgac cgttttttcc cttcaccctg
    49861 agatttcagc agagaaagga acatcctgac aacaccttcc atcgtcttta atgtgacatt
    49921 tcctgtaaag ccgtcacaaa ccagcacatc tgtcttgcca gttaacaact cattggcttc
    49981 aagatagccg atataattga tagaagggat tgttttaagc accgctgagg catcccgaat
    50041 actgtcgaga ccctttactt cttcttcacc aatattgagc aacgccacgc gaggattggg
    50101 aatttccacc acctcttcag ccagaactga gcccataatg gcaaattgca ccaacattgt
    50161 actgtcacaa tcgacgttgg cccctaagtc aaggaccacc gttttgccct tttgctgatg
    50221 tggtaatacc gtcaccagcg ccggacgctc aatcccctcc aggggcttga gtaataattt
    50281 tgccagcccc atcagtgccc cggtattacc ggcactgaca caggcttgcg ctcgaccttc
    50341 tttcaccagc tccagggcca cgcgcattga actcccacga ctggcgcgga tagcttgcga
    50401 aggccgggca tcactggcga taactgactg cgcaggaata atctgcagac gcgaacgttg
    50461 ttcaaagtca gctttagcaa gtaatggcgt gatggcgtcg ggattgccga ctaaaagaag
    50521 agtgagttgc gaattagaat tcagtgcctg caatgctgca ggcactgtca cggaagggcc
    50581 aaaatcccct cccatgacat ctaacgccag ggttagacgt gtcaaggtat cgtcgccgcc
    50641 cggtttggtc tttccccagt tgcctgggga aaatcctcac taagcttcat cacgcagacg
    50701 cgtgattact tagcgatgac cttgcggccg cggtagtaac cgtcggcagt gatgtggtga
    50761 cgcaggtgtt tttcaccaga agttttgtct acagacaggc tggtgactgc ggtcagcgcg
    50821 tcatgggaac gacgcatgcc acgtttggaa cgggttggtt tattctgttg tacggccatg
    50881 gaccttactc ctcaattacg gtttatcccc gatccaacgg ggagcaccaa ttacttacgc
    50941 tttaagctgg ctaatacggc aaatgggttt ggcttttgca cttcttcagg cagttcacca
    51001 aagaccatgt ccgcttcgga cacttcacag tgttcagaat catgcaccgg aactaccggc
    51061 aaggcgagga tgatttcatc ttcaaccatt gcaagcagat cgatttcacc gaattcgtta
    51121 acctcaatcg gttcatacgc ttccggcagt gcttcagcct gttcgtctga acgcacagga
    51181 ctaaaacaat acgttgtgta gacctgatga gtaaacggct tcccgcaacg ctgacactcg
    51241 agcgttaccg tcaccttcgc atcgccgttt aacactgcga gacgttggtt atcgatagcg
    51301 aacgacatgg agcattccac atcactgtcc acactgacta cggattcggc gacgcgctca
    51361 acctgatcag gggtatagat accctggtaa tcaaggcgtt tttgagccgt acgaaccgga
    51421 tcgagagtca ggggtaattt taccttttgc atagggcgcg catattaact ttgtaacgtc
    51481 atagagtcaa agaaaaaggc agcctgtggt tgccttttgc caataattcg cacacattgc
    51541 gggttactgc tttattttcg ttcagtgctg ataacgaaca tcaacctgca aaacgaagcg
    51601 catagtttaa aatggctatc atcaatacgc tatatctgat ggaaaaaaat atgcctaaac
    51661 ttattttagc ctccacctcg ccatggcgtc gcgctcttct ggaaaaactg caaatctctt
    51721 ttgaatgtgc agcaccagag gtcgacgaaa ccccacgcag cgacgaatca ccgcgacagt
    51781 tggtgcttcg actggcacaa gaaaaagcgc aatctctggc gtcacgttat ccggatcatt
    51841 taattattgg ttcagaccag gtatgtgttc ttgatggcga aatcaccggt aaaccgttaa
    51901 cggaagaaaa tgcccgtctg caattacgca aagccagcgg caatatcgtc accttctata
    51961 ctggactggc gttgtttaat tcggcgaatg ggcatctaca aacagaagtg gagccttttg
    52021 acgtccattt ccgtcacctg agcgaggcgg agattgataa ttacgtgcgt aaggagcatc
    52081 ccctgcactg cgcgggtagc tttaagagtg aaggatttgg cattacgctg tttgagcgtt
    52141 tagagggacg tgaccctaac acgctggttg gtttaccgct aatcgcgctg tgtcagatgt
    52201 tacgtcggga agggaaaaac ccgctgatgg gatagttgtt tgcgctggat gcggttacgt
    52261 cgcatccggc aatcggtgca ggcggcctga tgcggcgtaa acgccttatc aggccaaccg
    52321 cccattatca ggcttatatg cttgtattag cgcgcgttac gcagcttttg caaacaacgc
    52381 ttcaaacctt catccatcgg cgcttcgata cgcatcacct caccggtccc cggatgggta
    52441 aacttcaacg ctgcggcgtg caggaacaga cgatttaatc ccgtgcctgc ttcagtgagc
    52501 tgtctgtcaa attcacggtc accgtagcga tcgtcaaagg caatcggatg acccgcatac
    52561 tgtgtatgca cacggatctg atgagtacgt ccggttaccg ggctacaacg caccagggtg
    52621 gcaaatgcat agcgttcttc cactttaaag cgtgtttccg acggtttgcc ttcctgactg
    52681 acacgcacga tacgttcgcc gctttgcaga atatttttca ataacggcgc ttgaacgctc
    52741 ttcacatgcg actgccactg accgcgcacc agcgccaggt aatctttttg cattcctttt
    52801 tcacgtaatt gctcatgcag agaacgcaac gccgagcgtt ttttcgctat cagcaacaca
    52861 cctgaagtgt cccggtcgag acgatgaacc agttcaagga accgcgcttc cgggcgcaac
    52921 gcccgcaaac cttcgataac gccgaagctt aaaccactgc cgccatgtac cgccgtaccg
    52981 gaaggtttat tcagcaccag gatatgatca tcttcatata agatgacgtc cgccagcgcc
    53041 gccacctttt gcagatgtgg cgaaaccgcc tcttcttccc gctcagcaac gcgaaccggt
    53101 gggatacgca cctcatcacc cgcttcgagt ttatattcag gcttaatacg ttttttgttc
    53161 acccgcactt cgcctttacg caaaatacgg taaatcatac tttttggtac gcctttcaat
    53221 tgggtacgca aaaagttatc gatacgttgc cccgcttcgt cagcggtgat agcaacaatt
    53281 tttacggatg gagtctctgt tttcatggtg ggcgattcta attagccaac aggattcgcg
    53341 ccactcattt ttctatgctt atatttagtt ttgcacctta ttacttcact acgtgatcac
    53401 tttattgatg gttattaaac caatcaccag caagaagtga aaaaactgtg agtaagcggg
    53461 tgataaatgg taaaagtcat cttgctataa caaggcttgc agtggaataa tgaggccgtt
    53521 tccgtgtcca tccttgttaa aacaagaaat tttacggaat aacccatttt gcccgaccga
    53581 tcatccacgc agcaatggcg taagacgtat tgatctttca ggcagttagc gggctgcggg
    53641 ttgcagtcct taccggtaga tggaaatatt tctggagagt aatacccagt ctgtttcttt
    53701 gataattgcg ctgtttttcc gcatgaaaaa cgggcaaccg acactctgcg cctctttgag
    53761 ctgacgataa ccgtgaggtt ggcgacgcga ctagacacga ggccatcggt tcacacccgg
    53821 aaaggcgtta ctttgcccgc agcttagtcg tcaatgtaag aataatgagt aagttacgat
    53881 gaaaagaatg ttaatcaacg caactcagca ggaagagttg cgcgttgccc ttgtagatgg
    53941 gcagcgtctt tatgacctgg atatcgaaag tccagggcac gagcagaaaa aggcaaacat
    54001 ctacaaaggt aaaatcaccc gcattgaacc gagtctggaa gctgcttttg ttgattacgg
    54061 cgctgaacgt cacggtttcc tcccactaaa agaaattgcc cgcgaatatt tccctgctaa
    54121 ctacagtgct catggtcgtc ccaacattaa agatgtgttg cgtgaaggtc aggaagtcat
    54181 tgttcagatc gataaagaag agcgcggcaa caaaggcgcg gcattaacca cctttatcag
    54241 tctggcgggt agctatctgg ttctgatgcc aaataacccg cgcgcaggtg gcatttctcg
    54301 ccgtatcgaa ggcgatgacc gtaccgaatt aaaagaagca ctggcaagcc ttgaactgcc
    54361 ggaaggcatg gggcttatcg tgcgcaccgc tggcgtaggc aaatctgctg aggcgctgca
    54421 atgggattta agcttccgcc tgaaacactg ggaagccatc aaaaaagccg ctgaaagccg
    54481 cccggctccg ttcctgattc atcaggagag caacgtaatc gttcgcgcat tccgcgatta
    54541 cttacgtcag gacatcggcg aaatccttat cgataacccg aaagtgctcg aactggcacg
    54601 tcagcatatc gctgcattag gtcgcccgga tttcagcagc aaaatcaaac tgtataccgg
    54661 cgagatcccg ctgttcagcc actaccagat tgagtcgcag atcgagtccg ccttccagcg
    54721 tgaagtccgt ctgccgtccg gtggttcgat tgttatcgac agcaccgaag cgttaacggc
    54781 catcgacatc aactccgcac gcgcgacccg cggcggcgat atcgaagaaa ccgcgtttaa
    54841 caccaacctc gaagctgccg atgagattgc tcgtcagctg cgcctgcgtg acctcggcgg
    54901 cctgattgtt atcgacttca tcgacatgac gcctgtacgc caccagcgtg cggtagaaaa
    54961 ccgtctgcgt gaagcggtgc gtcaggaccg tgcgcgtatt caaatcagcc atatttctcg
    55021 ctttggcctg ctggaaatgt cccgccagcg cctgagccca tcactgggtg aatccagcca
    55081 tcacgtctgc ccgcgctgct ccggtaccgg taccgtgcgt gacaacgaat cgctgtcgct
    55141 ctctattctg cgtttgatcg aagaagaagc gctgaaagag aacacccagg aagttcacgc
    55201 cattgttcct gtgccaatcg cttcttatct gctgaatgaa aaacgttctg cggtgaatgc
    55261 gattgaaacg cgtcaggacg gtgttcgctg cgtgattgtg ccaaacgatc agatggaaac
    55321 cccgcactac cacgtgttgc gcgtgcgcaa aggggaagaa acgccgacct taagctacat
    55381 gctgccgaag ctgcatgaag aagcgatggc gctgccgtct gaagaagagt tcgctgaacg
    55441 taagcgtcct gaacaacctg cactggcaac ctttgccatg ccggatgtgc cgccagcgcc
    55501 aaccccagct gaacctgccg cgactgtcgt agccccagca ccgaaagctg caacggcaac
    55561 accagcagct cctgcacaac ctgggctgtt aagccgcttc ttcggcgcac tgaaagcgct
    55621 gttcagcggt ggtgaagaag ccaaaccgac cgagcaacca acaccgaaag cagaagcgaa
    55681 accggaacgt caacaggatc gtcgcaaacc tcgtcagagc aaccgccgtg accgtaatga
    55741 gcgccgcgac acccgtagtg aacgtactga aggcagcgat aatcgcgaag aaaaccgtcg
    55801 taatcgtcgc caggcacagc agcagactgc cgagacgcgt gagagccgtc agcaggctga
    55861 ggtaacggaa aaagcacgta ccaccgacga gcagcaagcg ccgcgtcgtg aacgtagccg
    55921 ccgccgtaac gatgataaac gtcaggcgca acaagaagcg aaggcgctga atgttgaaga
    55981 gcaatctgtt caggaaaccg aacaggaaga acgtgtacgt ccggttcagc cgcgtcgtaa
    56041 acagcgtcag ctcaatcaga aagtgcgtta cgagcaaagc gtagccgaag aagcggtagt
    56101 cgcaccggtg gttgaagaaa ctgccgctgc cgaaccaatt gttcaggaag ctccagctcc
    56161 acgcacagaa ctggtgaaag tcccgctgcc agtcgtagcg caaactgcac cagaacagca
    56221 agaagagaac aatgctgata accgtgacaa cggtggcatg ccgcgtcgtt ctcgccgctc
    56281 gcctcgtcac ctgcgcgtga gtggtcagcg tcgtcgtcgc tatcgtgacg agcgttatcc
    56341 aacccagtcg ccaatgccgt tgaccgtagc gtgcgcgtct ccggaactgg cctctggcaa
    56401 agtctggatc cgctatccaa ttgtacgtcc gcaagatgta caagttgaag agcagcgcga
    56461 acaggaagaa gtacaagtgc agccgatggt gactggggtc cctgtcgccg ccgctgtcga
    56521 acctgttgtt agcgcaccgg ttgttgaaga agtggctgaa gttgtagaag cccccgttca
    56581 gattgccgaa ccgcaaccgg aagtggttga aacgacgcat cctgaagtga ttgccgccgc
    56641 agtaactgaa cagccgcagg tgattaccga gtctgatgtt gccgtagccc aggaagttgc
    56701 agaacacgca gaaccggtgg ttgaaccgca ggaagagacg gcagatattg aagaagttgc
    56761 cgaaactgct gaggttgtgg ttgctgaacc tgaagttgtt gctcaacctg ccgcgccagt
    56821 cgtcgctgaa gtcgcagcag aagttgaaac ggtagctgcg gttgaacctg aggtcaccgt
    56881 tgagcataac cacgctacgg tgccgatgac gcgcgctccg gcaccggaat atgttccgga
    56941 tgcaccgcgt cacagtgact ggcagcgccc tacttttgcc ttcgaaggta aaggtgccgc
    57001 aggtggtcat acggcaacac atcatgcctc tgccgctcct gcgcgtccgc aacctgttga
    57061 gtaataatta gctcaagtaa tcaagccctg gtaactgcca gggctttttt atttcatctt
    57121 tgaatcaaca tattaaaatt catcgaatgg catccttgct aaccaacaat gcaaaatagg
    57181 caaaaaaatg cccgccccgg cgatcccaga gcaggcagac aaaacatacc cagtttcatg
    57241 atatgtttta aagcgaaatt atttgttgag ctggaagagc gacaatccct gcatatcggt
    57301 aaatgcttta tacgatgcct gcaatgccgt ttgctgcatg atgtaagatg aaatagttgc
    57361 attccagtcc acatcaacca gatcgctcat ctgctgcgtt tgccctaaag cgcgatcgct
    57421 acctaatgaa tccagcgact ccagttcgtt cagctgcgtg cctaattccg cgcgaacggt
    57481 cagcacattg ttcagcgagt ttttcagtcc gcggttggtt ttatctaacg ccgcagcggc
    57541 ggtttcttta tccgcttcgc tatccgcgac cggcgttttc agggctgcga tggcgctatc
    57601 cagcatggca aaaagattgg tttcagaagc gctaccgtct ggttccgcta ccgcgttact
    57661 ggtaatactg tcgaaaattt tgtcgcccgt atgtcctatc accatcgaac gcgaagcatc
    57721 gacctgttgc ttaatacttt ctgttccgcc gacataatca ccgttagcct cactaaacgg
    57781 cgcagtctct gttttataac cggcaaaaat gtagcgccca ttaccgtcag tggtgttcgc
    57841 cagattcagc aactggtcac gaagcccctg aatatccgta gccagcgagg cccggtcatc
    57901 gtcactcaag gtgccattgc tggcgtagac aattttttcc tgagcattct ggatagcagt
    57961 ggtgacctgg ctaagtacac tctcttccag tgacactttt tgagtggcga aagtacgcgc
    58021 cagcgtgtac tggctgtttt gcgcctgtgc ctgggagaga acaaccgctt gtgatgcagc
    58081 aatgggatca tctgaagggt taacgactcg cttacccgtc gacatctgtt cgccgtactt
    58141 catccattct gcctgagaat tggtgatacc acgcatgttt tgctggtaca tcatctgggt
    58201 actgaaacgc attttatctc cccttagcga atgttaatca gcgcatcaaa aatcgcgttt
    58261 gccgtctgca gaacctgcgc attcgccagg taatactgct gaaaacgttg cagatttccg
    58321 tactcctcat cgagattgac accggaaatc gactgctgct gattggaaag ctgcgtcacc
    58381 acattacctt gcgtggtgct actggttttc aacgtcgcgg ttttattacc gatatcactc
    58441 actaacgagg cataagcgtc gttaaaggat ttcgcaccgc ccaccgtttt actattgctt
    58501 tgcagatcca gcagggcctg accgttgcgg ttatcgctat cacccgcatc tttttcgctc
    58561 gccatcgcta ttttcgcttc gtcggtgatt aatacatcca tgttgacgat ggcgtcactt
    58621 actggtttca gcgtgaagct gtcgttaacg gcaggcgttc ctgtaaacgt caactccaga
    58681 ccatcaaatg ccactttacc gttggcatcc ggcgtcaccg taaaagtggt attgctggca
    58741 aggcgggtga cctgccactg attattatcg aacgagattt tgtaatctgt cgccagtacc
    58801 acggaggcat cagttaccgt ggcaccgatc gcaacgtcac ctttgttttt cgtgttttgc
    58861 agaaccgcgg gcttaccgat agtaaagaaa tcttcaccag catcgccgtt agcatcaaat
    58921 ccggctttgt gttgggtgtt gaaagcctcg gcaaatgcca gcgccagttg tccaagcgta
    58981 ttacgcgtct ggtccagctc ctgagaacgg aatgtcagaa tgccgcccag cgacccggta
    59041 ttcagtaatt tctccgggat ctcaatattg cctgccgtcc catcaacata agcgacagtc
    59101 gtacgagaag ggtcagcgct ggaaggaact gccgccagtt gccgcgccgt acttccctga
    59161 accagtgagt aaccattggc caccgtgatg ttataagtgc cgccatcctg aacgctgact
    59221 tctacaccaa caatctggtt taattcgctc accagttgat cgcgttgatc cagcagattg
    59281 ttaggtgacg cccctgcccc cacgcctgtc aggcgcgaga tttgatcgtt caggctggca
    59341 atttgtttag cgtaattgtt gatctgatca acgctggcac ctatcgcgat attgacctgt
    59401 ttgtcctggt cgcgcagata ttgatcggtg gttttaaact gattcaccaa cccttctgat
    59461 ttcccaatca gcgcctggcg cgctgccggg tcttccgcgt tactcaccag cgtttgcagg
    59521 ctggtgaaga aatcctgcat ctgtgttgcc agcgaagagg tactggtgga gagcatattg
    59581 tcgattttcg acatctgctc atagcgggcg gtcaggccgc tactttgcgt ctgcgcagca
    59641 cgtaactggt tggtaataaa cgcatcatac tcacgctgca caccagaaac gtagacgcca
    59701 ttgccaaccc agccgccagc gcccaacgtg ctattggcct gcgccataat agtggtttgg
    59761 cgggtatatc cggcaacgtt atagctggag atattattac ttgccgtatt taacgccgcc
    59821 tgggccgcgt tcagtccgct catggcgtta ttgatcaagc tggacatgga ggttccttat
    59881 aagccttcaa ttacagagta ttatcggcag cgacccgccg gacttgagtt attcagaaca
    59941 gattatcaat gttcatgctg taggttttgc tcaccttgtc gcttatcgat ttcatctgct
    60001 ggatcatgtt ggtgagttta cgggcatagt gaggatcggt agcatacccc gcgtcctgta
    60061 gggcctgcgc cccctgctcc gcactcgcgg ctgtcgtcac ggcggcgtag cgcgggttac
    60121 gtgttaacag cccaacgtaa tccgacaatg cttccagata cgagctgtag acccgaaatt
    60181 tcgcttttac tttcttcgct tcgccatttt catattcagt cgtggtgatt tcagtaactg
    60241 gacctttcca gttgccagag gctttgacgc caaacaggtt atagctcggc tcgccgtttt
    60301 cgcggcggat ttgccgttgc ccccaaccag actccagcgc cgcctgagcg aggatcaaat
    60361 gatgtggcac accgctttgc tggcttgcca gttgggcggg cagcgagagt tgcgcgagga
    60421 atgctttact gtcacccggc agcgaatcat cgtagttacg tggcacggcc ttttgcacca
    60481 gctgcgaaag cgcctgattt tgataacgca ccacagtttc gagcgggaat ttcatcggtg
    60541 ctgctggcgt ggactcctct ggcaatggtt gttctggcgt catctgttta accatcatct
    60601 ctgcaagccc cagacctttg cccgccgtca tctgttgggc aatctgctgg tcatacatac
    60661 tggtatacag gcgagtgtgc tcgctgctga acaggccatc ttttggtaaa gcgtcgcgca
    60721 tgcttttcaa catcatctgc acgaacatcc cttccacctg acgggccacc ggacggatat
    60781 ttgccgccgg atcttcgccc gctttcgcct ttagttcgtt gagtgattgc gcatcccagg
    60841 ccgcacttgc cagtagtttg ctgtcgctga tcatcagatg atttccagtt ttgcccgcag
    60901 acatcccgca ctttgcattg attgcagtat ggacatcaga tccatcggcg tagcgcccag
    60961 cgcattgagc gcgcgcacca cgttattgag gctggcgctg gaacgtacgc tttgcagcga
    61021 accgccgctc tggcgtaaat cgatctgcgt ttgtggagta accacagtct gtccaccacc
    61081 aaacggtgta tctggctggc tgacattggc ctgacgattg actgttactg agagatttcc
    61141 ctgcgctacc gcgcagctgt cgagggtcac ttcgcgattc atcaccaccg aaccggtgcg
    61201 cgagttaatc actactttag cgtcctgcgg ggtgacatta acctgcatat tctggatatc
    61261 tgcaaggaaa cggacctggg aactgttgcc acttggtacg cgcacctgaa tagtccgcgc
    61321 atctaacgcg gtggcgctgc catatccacg cacgcggttg atggtgtcag cgatttgctg
    61381 cgccatgctg aaatcttcgt cgttaagttg caaattaagg gtattcccga cgccaaactg
    61441 gctgggcaat tcacgttcaa taaccgcacc attggtgatc cgtccaccgt tcagttggtt
    61501 cacctgaaca ctgctaccgc cagcggaggc tcctgcgccg ccaaccagaa tattgccctg
    61561 cgccagcgca tacacctgac tgtcaacgcc cttaagcggc gtcatcaaca acgtacctcc
    61621 acgcaagctt ttggcatttc ccatggaaga aaccaccaca tcgatggttt gcccctgacg
    61681 tccaaacgga ggaagtgact ctgtcaccat taccgcagcg acgtttttta gctgcatatt
    61741 ggtgcccgtc ggaacggtaa ttcccagctg tgagagcatg ttattaagcg tttgtgtggt
    61801 aaacggcgtc tgggttgtct ggtcaccggt gccatccagc cccaccacca gaccatagcc
    61861 aatcagtgag ttttgcctta ccccctgaac actggtgaga tcgcgaatac gctcagcctg
    61921 agccgccgtc gtaaccagta gaagaattaa tgcagagaga aatttaatca ccacagcctc
    61981 acttacattg gcgacaggtt aaggaagaaa cgctgcaacc agcccatatt ttgcgcttcg
    62041 ttaatgtagc cattgcctac gtattcaatg cgcgcatccg ccacctgagt agacggtacg
    62101 gtattgctgc cgctgatagt gcgtggatta accacgccag agaagcgaat aaattcggta
    62161 ccctgattaa tggcaatctg tttttcaccc accacatgca ggttgccgtt gaccagtacc
    62221 tggtcaaccg tcaccgtcaa cgtgccgcta aaggtattgc tggcattggc cccgcccttt
    62281 ccgttgaacg tgttaccacc ggaggcttcg acatcggcac gagcgttacc aaacagcccc
    62341 tgcaaatagc gcggcacagt atcaaagcca aaattagttt taccgtcacg gctggcattc
    62401 gcagaggagc ttttgctggc gctgacgttc tcctgcaaca cgatggtcag cgtatcgcca
    62461 atattgcgtg gtcgacgatc ttcaaacagc ggttgatagc catagttaat cggctgagca
    62521 gactggaaaa tagaaccgtt ggcgacgggc gtcggaccgg gaaccggttg tgcactggtc
    62581 gccccctgca ccagcggcgt ggagggtatc caggcgcagc cggttagtga aagcaccaac
    62641 aagctggaaa tggcataagt atgcgcagcg tttttttgca tggctatcat cttcaaaaat
    62701 cagtaaaccg gtgaacctgc caccggttaa gccttagagt tgcgtcagtt tttgcagcat
    62761 ctgatcggtg gtggacaccg ctttactgtt gatttcgtaa gcgcgttgca cctgaatcat
    62821 attgaccagt tcttccgcca cgttgacgtt agacgtttca acataccctt gatacagcag
    62881 tcccgcgccg ttcaggcccg gcgtgctttc gttcggtaca ccagaggatt gcgtttcggt
    62941 gtagaggttt tcgccaatgc tctccagccc ggtgtcattc ataaaggtgg tgagattgag
    63001 ctgcccaacc tgaaccggag ctgcctggcc ttgttgggtt acgctgacca cgccatcacg
    63061 accgatggtg atacttaacg cattcgccgg aatggtgatc gctggctgca cctgaaaacc
    63121 accagctgcc cgttctgatc cacctggaaa gagccgtcac gggtataggc tgatgaacca
    63181 tctggcaaca tcacctggaa aaagccctgc cctttaatcg cgacatcttt gctgttgttg
    63241 gtctgcgaca ggtttccctg gctgtgtaag cgttcagtgg cgaccgggcg tacccccgtg
    63301 ccgatttgta atccggaggg taaggtggtt tgttcggaag actgtgcccc cggctggcga
    63361 atggtttgat aaagcagatc ttcaaacacc gcgcgctgac gcttaaaacc gttagtactg
    63421 acgtttgcca ggttgttggc aatgacgtcc atattggttt gctgggcgtc aaggcccgtt
    63481 ttggcgatcc ataatgaact gatcataaaa tgtcctgtag tggattagtg acaaagtgca
    63541 tatgtcttct tgccggatgc ggcgtaaacg ccttatccgg cctacaagtg ctcgcaaaat
    63601 cgataaattg cgattgcccc catagacccg ataaacttgc gcatcgggca acttatactt
    63661 gtcatgtatc ctttcaatta actcatcgac agcagttggt tggcacggcc tgcgttatca
    63721 tcgacgctgc tgatcacctt catctgcatt tcaaaacgcc gcgcgctggc aatcatgtcg
    63781 ctcattgccg caacggcatt gacgttactg ccttccagaa cccccgacat cacacgcaag
    63841 gttggatctg cctgcagtac cggccctcgc gtggcctggg tttctgcgct taaacgaaaa
    63901 atgccgtcgt caccgcgctg cacttcgctg cccgtggctt tcaccagttt cagacgccct
    63961 actggcgcaa ctgtatttgc cggatcgccc ggattgagcg ccgagattgt gccatcggca
    64021 gcaatagtga tttccgctcc ttccggcaca gcaattggcc ccgcctcgcc tatcacctga
    64081 tgcccctgaa ttgtcagttg cccggtggga tcaacctgaa tgctgccatt acgcgtatac
    64141 ccttcgctgc cgtcagcggt ctgcacggcc agccagccat cctgctgcaa cgcgacgtcc
    64201 agcgggcgcg aggtgtaatc cattttgccg ggcgtcatat ctgcgcccgg cgttgacgcc
    64261 gtgaccaacg tgcgcgtggg cagagaaagc ccttccactg gcaccgcgcg taaagcattc
    64321 aactgcgcgc gaaaaccggg cgttgaggca ttggccagat tactggcggt taccgcctgt
    64381 tgattcagtg tctggctggc tgctcccatc gcggtataaa ttgcgtgatc cattgagcta
    64441 tcccgtcagc gattagcgta agttaaccag cgtgttgagg atctggtcct gagttttgat
    64501 ggtctgggcg ttagactgat agttacgctg ggcaacgatc atattgacca gttctttact
    64561 gagatcgaca ttggacgctt ccaacgcacc gttggtcagg gtgccaaagt tgcccgtccc
    64621 ggctgtcccc aacagcgcca cgccagaaga ttgcgtcgca gaccagacgt tgtcgccttc
    64681 ggatgccaga ccttcgttgt tggcaaagtt cgccagtaca atctgcccca gcagttgtgt
    64741 ttgttcgttg gaatagttgc cgacaaccgt accgtcatca ttgatttgat aactcaccag
    64801 atcgcccggt ttgtagccat tctgggtggt tgccacaata ttgttagcgc cggtattttg
    64861 ctgcatggag ttgaggaagc tcaggctaaa cgtggcggga tctgcgccgt taattgcgcc
    64921 agtggtaata ttctctgttg gatttgaggt cagaacgcca ttggcattaa acaccagctt
    64981 cattgcaggc tcggctgtac ctgttggatc actgctatcc tgggtgtaga cctgccagtt
    65041 attatccccg gtcttcacaa agtagacgct catgtcatga gcattaccct gactgtcgaa
    65101 aacagtcacc gaaccttttt tgttatagct atccgcattg ctggcgctga atggcgtaac
    65161 agtaggaagc ggatcactgg aattcaggtt gatctgcatc gacgccgtgg tggtagtttt
    65221 cgctgccatc agggtattcg ggatcgaaat attggtcgga ttcgcccctt gctgaatagt
    65281 cggcggcgta ccggttgccg ggtaacccgt cagctgtaaa ccttgcatat tcaccaggtt
    65341 acggttttca tccagcttaa attgtccgtt acggctgtag aacaccgaac cgttgctgtc
    65401 taccagacgg aaaaaaccgt tctggctgat agcaacgtcc agacctcgcc cggtgttggt
    65461 ggtcgtgcca tcggtaaagt cctgagtgat accggcaact tttaccccca gtcccacttt
    65521 cgaaccggca aacatatcgg caaaagaggc cgtgcctgat ttaaagccgt aggtggcgga
    65581 gttggcgata ttgttgccaa taacatcgag gttggtggcg gcagcgttta atccgctaac
    65641 cgcttgagaa aaggccatga ctgactcctg aaatgtgaag gcttaaatta tctgccgtac
    65701 ttcgtcgagg gtggtggtgc cgtaagtgcc gagatccagc gtattaccgc tgttgccgcg
    65761 gagcacaccc tgcaccagag caaactgcag cggctgggca accagttgtg taccaccgtt
    65821 actggcgcta atcgctacat tgtaagaacc gttcggcgca gtgctgccat cagtcaacgt
    65881 accgtcccag gtgaaactgt ggactccggc ggtcagttca ccaatatcaa tggtgcgcac
    65941 aaccgcgcca tttttatcgg tgatggtggc cgtaactttg tctgccgcct gttgcagctc
    66001 aacaccaaac ggcgtggtcg tggtcacagc cccttcttca ctgccggttc ccgcaagaac
    66061 agtggtgccg gggatcatca cgccgtgacc gatcaggtta ctggcctgta acgactggct
    66121 gttatcaatc tgtccggaaa tagatccgag cgtggtattg agtttttcaa tcccgctgac
    66181 cgtgctgatt tgtgccaatt gcgacgtcag ctcgttgttt tccattggat tggtcgggtc
    66241 ctggtttttc agctgcgcca ccaacaaagt cagaaaactg ctttgtaagt ctgcggcgtt
    66301 gctgcccgtg agcgaactac tgctggtggt actgacgccg gtatttgtcg gatcggtggt
    66361 ggttaccgca atggacatag ctttctcctt tattgaccga gcgtaagggt tttcagcatc
    66421 atgcttttca ccgtgttgag cacttcaaca ttggcctgat agctgcgtga cgccgacatg
    66481 gtgttaacca tctccccgac gacatcaacg ttcggcattt ttacgtagcc ctttgcatcc
    66541 gccagcggat tacccggttc ataaaccagt ttgtccgggg cctgactttc tataacatcg
    66601 gcaaccttta cgccgcctgt cgcagcacct ggtgcagcgt taacctggaa taccacctgt
    66661 tttgcccgat atggctgtcc atcgggaccg gtcacgctat cagcattcgc cagattactg
    66721 gccgccacgt tcaagcgctg ggactgggca gttaacgccg acccggcgat atcaaaaata
    66781 ttcagcagtg ccatccgtta atttccgctc tgtaaaacgt tcatcatgcc tttgatttgc
    66841 ccgctcaacg cgctaaggct catctggtat tgcaggctgt tatcggcaaa ctgggtgcgt
    66901 tcgcgatcca tatcgacggt attaccgtca agcgaaggct ggtccggaat acggtattgc
    66961 agttctgcgg taggaggcgt cagcgcctgc gccggaatgt gttgcgttga ggtcatcgtc
    67021 agtgcaacca cactggttgc atcccgtcca cgttgcatga cttttttaag ttcactggca
    67081 aaatcgatat cgcgcgcctg ataaccaggg gtatcggcat tggcgatgtt tgctgccagc
    67141 acttcctgac gctgggcgcg cagattgagc gcctcttgtt gaaaacgtaa ggcggcgtcg
    67201 agcttatcga gcatatctcc tccgcaggta tcaaaattct gccatcagct taaatgcctt
    67261 tacgcgcgcg ttatcggcgg aataaacgca aaatgggtcg ctatttatgc cgttgatgac
    67321 cattgcggac aggtacaatt cacgttgtag aaatggctgg gggcgaaaat gctggcaata
    67381 aaacgtagcg tggcgatcat ctcgatacta ttcactccgt taagtgcggc gagtaatctc
    67441 acatcgcaat tgcaaacctt ttttagcgcc cagctcgcgg gggtaagtga tgaggttcgt
    67501 gtttctattc gtacagcgcc caatctacta ccgccgtgcg agcagccatt gctttcgatg
    67561 agcaataatt cccgcctgtg gggcaatgtg aatgtgttgg cacgctgcgg taacgacaaa
    67621 cgatatttac aggttaatgt acaggccaca ggaaattatg tggttgccgc gatgcccatt
    67681 gtgcggggag gaaagctgga agctggcaat gtcaaactaa aacgcggacg gcaggatacc
    67741 ctgccaccgc gtacggtgct ggatatcaat caacttgttg atgccgttag cctgcgcgat
    67801 ctatcacccg atcaacctat ccagttaacc cagtttcgcc aggcatggcg ggtaaaagcg
    67861 ggacaacgcg tcaacgtgat cgccagcggt gacgggttta gcgccaacgc agaaggtcag
    67921 gcgctgaaca atgcagccgt cacacagaat gcgcgggtgc gcatggtatc gggactggtg
    67981 gtcagcggcg ttgttgatgc agatgggaat attcttataa accagtaagc tgttaaagat
    68041 tacccgtccc ttgccgataa ataagcaaca catgataaaa gcgccctcaa tgaggaataa
    68101 accatgagta ttgatcgcac ttcgcctctg aagcccgtaa gcaccgttca accacgcgaa
    68161 accactgacg cgtcggtaac gaacacccgg gcggcaaaaa caacagcctc caccagcacc
    68221 agtgtgacgt taagcgacgc gcaagcaaaa ctgatgcaac ccggcagcag tgatatcaat
    68281 cttgaacgtg tcgaagcgtt aaaactggcg attcgtaacg gtgaactaaa aatggacacc
    68341 ggcaaaattg ccgatgcgct gctcaacgaa gcgcagcaag acttgcagag taactgatcg
    68401 tatgacacgt cttgcagaga tcctcgacca gatgtccgct gtgcttaacg atctcaaaac
    68461 ggtaatggat caagaacagc aacatctctc tatggggcag atcaacggca gccagttgca
    68521 atggattaca gaacaaaaaa gctcactgct ggcgacgctg gattacctcg aacagttacg
    68581 caggaaagaa cccaatacag caaatagcgt tgatattagt caacgctggc aggaaattac
    68641 tgtgaaaacg cagcaactac gccaaatgaa tcaacataac ggctggttac tggaaggaca
    68701 gattgagcgc aatcaacagg cgctggaaat gttgaaaccg catcaggaac cgacgctata
    68761 tggggcgaac ggtcagacct caacaaccca tcgcggcggt aaaaagattt cgatctgaat
    68821 attatctccg gcctgcactg caggccggaa tgcattgtta caccgtccgg cgggcaaatt
    68881 ctttaacttt gaagcccagt accgccagtg cagcgaagta cgcggcaatc cccgccagca
    68941 cgaccgccat taaacgcagt aagcgccagg gcatggtacc caatgaccac tccggcatga
    69001 tatgtaacat acctaaaagc acgccagaca tcaccagtac cgccaccacc agacgcaaca
    69061 gaaacgccat ccagccgggt tgcggagtaa agattttctg cttacgcaac tgccagtaaa
    69121 gcagcgaagc attcagacac gccgccagac caatagaaag tgacagccca gcatgtttca
    69181 acggaccaat aaacgccagg ttcatcaatt gcgttaaaat cagcgtaacg atggcaattt
    69241 tcactggcgt tttaatgtcc tggcgggaat aaaagccagg agccaacact ttcactacaa
    69301 tcaggccaat caaacccacc gagtaggcaa ttaacgcccg ctgggtcatc agcgcatcaa
    69361 acgcggtaaa tttaccgtac tggaacagcg aaacggttaa cggacccgaa agaatgccca
    69421 acgcaaccgc actcggcagc gccaacagga aacaaagacg caacccccag tccatcaaac
    69481 ggttgtattc atcatgattg ccactggcaa aacttttcga cagcgacggc agcaaaatgg
    69541 tgccaagcgc cacgcccagc acaccggagg gaaactccat taagcggtcg gcgtaataca
    69601 tccaagacac cgaaccggaa gcaagaaacg aggcaaaaat ggtgttgatg attaaggaga
    69661 tctggctcac agagacgcca aggatcgccg gtcccatctg tttcaccacg cgcattgctc
    69721 cggcatcgtg gaagttaatg cgcggcagga ccagcatgcc gatcttcttc aggtgcggta
    69781 gctgatacac cagctgcagg atgccgccga ccgtaacagc ccacgccagc gccagcaccg
    69841 gtgggttaaa gtacggtgcg gcaaacagcg cgaaaccaat catgctgatg ttaagcagtg
    69901 ttggcgcaaa cgccggaatc gagaagcggt tccacgtatt cagaatcgct cccaccagcg
    69961 acgccaggga gatcagcaag atatagggaa aggtaatctt tagtagctgg ctggtcaggg
    70021 caaatttgtc agctgtgtca gcgaagcctg gcgcggtcac catgatcacc cacggtgcgg
    70081 cgagcatgcc agcgaccgtc acaaccgcca gcgcaagtgt cagcaggcca gaaacataag
    70141 agacaaagac ccgcgtggcg tcttcaccct gcttactttt atattccgcc agaatcggta
    70201 caaatgcctg ggaaaatgcc ccttcggcaa agatacggcg taacaagtta ggaagtttaa
    70261 aagcgacgaa aaaggcgtcg gttgccatcc ctgcgccaaa gattctggcg acaattgcgt
    70321 ctcgtgcgaa gccaagcaca cgcgaaaaca tggtcatcga gctgacggcg gccagcgatt
    70381 ttaataaatt catcggtgtt ctaatccaga cccacgggca aaacgcctgc aaggcaggcg
    70441 ctgaaagagg cgaatagtct acctggatta tggtgaattg ctaccgccag atgttacagg
    70501 gtttattcac tcatcgcatc gcgccagatc ttgtcaacga tacgttgcgc cagcactgcc
    70561 tgttcgccgg cggtttgcag aactgtctga ttttgcacac attcaatgaa gtgccgcgca
    70621 cagccgacaa atccacgttg ctcaagagtg ctctgccaac caggaatcgg tttatgcact
    70681 acgccctgcc cgcgctcctc acgccattcg cgcatatccg taatgtcgat gagcgcatcg
    70741 tcagtcacgg cctgcacggt ttcacgctga cttccggcac ggcgatgcat acaggtggtg
    70801 atctgcaaag gatcggccga aaaatggtgc tcggcaaaca gcatttcgcc agcgtcgtta
    70861 gtcagtagcg taccgccatc cagagaggct ttgccgcccg acaaccacag cgcggtatcc
    70921 accacatgca gataatcatc cagcaacgtg aaataaagat cgtgtggccc gacactattg
    70981 ctacgatgtt tatccattct tagcgaagct gcggtagcga gttgcgtttt taactcaccg
    71041 tagagtggtg cgaaacgacg gttaaaaccg accatcaacg tcagtttttt ccgcgccgcc
    71101 agttccacca gccgttcagc atcgcgcaga ttttctgcca gcggtttatc gacacagaca
    71161 tgtacacccg cattgagtaa cgtactgacc acgtcaaagt ggctggcggt gctggaatgc
    71221 acaaaaaccg catcgcaact ggcggcaagg ctggataacg aatcggcata aggaatgcgc
    71281 cagctttcac aaattggcag ggctttcgcg cgcgtaggcg accaggctcc ttgtaacatc
    71341 cagtcagacg ctgccgccag caccggtaac cacgcttttt gcgcaatgcc gcctaatccc
    71401 acaacgccga tacgtaattt tttcacttta atctcccaga tgggccagca acgaatcaag
    71461 acgctgtttc agttctgcca cttcgatttc cagggcttcg acgcgggcct gtaaatcacc
    71521 atcaaccgcg ttcgacatat ccgttaccgc cggctgatcc tctacctcac cactgaaaag
    71581 atgcatgtag cggctttcgc gtttacccgg ttcgcgggcc agacgcacca caaaaggacc
    71641 atcttcgcga tttgccagtt gttccagcgt cgactccact tccgccatat cgctgaattc
    71701 atacattcgc gcggcgcggc tgcgcagttc acccggcgtc tgggcaccac gcaataacaa
    71761 cgtggtgatt aacgccactt ccgctgcgct cagtttcaga tcgccaaatt ctgaattaca
    71821 aaaacgttgt tcatatttgg tgacccgatt accaaagcca ctcaccgtgc gcagataatg
    71881 gcgtttgacc agattatcca gttgctcctg cacttcagat tcactcagat tcatgaccgg
    71941 ttcacggttc gttttctgat tacaggccgt gactacacca ttgactgaga gcgggtattg
    72001 ctccggcgtg gtcacctgct tttccagcaa gcaaccaatc acgcgagctt caagggcagt
    72061 aagttgatat ttcatgagat tctccttagc ggccgggcgt ccagtctggg gtagttaatg
    72121 ccgtcagtac gtgatcgcgc cattgtccat caatcaacag atagtctttc gcatagcctt
    72181 ctttttcaaa acccagtcgc gccagtaaat caccgctgcg tttattgtgc ggcatataat
    72241 tagccataat gcgatgaata tgttgggtgc gctgcatata acgaatggct gcggtcaggg
    72301 cttcaaacat gagtcctttg ccctgccatt tttgcccaat cgaataaccg agatagcagg
    72361 catgaaaaga gccacgaaca acattggaaa aattggcaac gccaataatc tctttttcgt
    72421 ccgggtcgaa taagccaaag tagaaagctg aaccttgttt atgaaattcg ttaatcatcc
    72481 ccagcctggc ctgccagcct gatggataac agtggctttc gtcgcgcact ggctcccagg
    72541 gcttgaggaa atggcgattc tctgcgtaat aatccgcaag acgccaggca tcacgatcat
    72601 gcaccagacg cacgaccagt cggtctgtgg ttaagcgcac ttttggcacg ttactgcgat
    72661 agccaaacat tctataccta ctccttcccg taacgtcttt aatacgctgt aagtattact
    72721 atacctgcgc aaccagaccc tgtgaaaagt ccaaaatgcc attttctgca ttattcacat
    72781 atttgataaa aactctctat caaagcgacg ttttgataaa aaaatattgt ccccgaagtg
    72841 atgggaaaag tcttagcggc aatgcagaat agaaagtgct catcttttat ttcccgggag
    72901 gggaaatgtc ccgcgtatcg caggcgagga acctgggtaa atatttcctg ctcatcgata
    72961 atatgctggt cgtgctgggg ttctttgttg tcttcccgct gatctctatc cgcttcgttg
    73021 atcaaatggg ctgggccgcc gtcatggtcg gtattgctct cggtctacgc cagtttattc
    73081 agcaaggtct gggtattttc ggcggcgcaa ttgccgaccg ctttggtgcc aaaccgatga
    73141 ttgttaccgg tatgctgatg cgcgccgccg gattcgccac aatgggtatc gcccacgaac
    73201 cgtggctatt gtggttttca tgcctgctct cgggactcgg cggcacgttg tttgatccgc
    73261 cgcgttcggc gctggtggtg aaactaatcc gtccacaaca acgttgtcgt tttttctcgc
    73321 tgttgatgat gcaggacagt gccggtgcgg tcattggcgc attgttgggg agctggctgt
    73381 tgcaatacga ctttcgcctg gtctgcgcca caggggccgt tttgtttgtg ctgtgtgcgg
    73441 cgttcaatgc gtggttgtta ccggcatgga aactctccac cgtacgcacg cccgttcgcg
    73501 aaggcatgac ccgcgtgatg cgtgacaagc gttttgtcac ctatgtcctg acgctggcgg
    73561 gttactacat gctggctgta caagtgatgc tgatgctgcc aattatggtc aacgacgtgg
    73621 ctggcgcgcc ctctgccgtt aaatggatgt atgccattga agcgtgtctg tcgttaacgt
    73681 tgctctaccc tatcgcccgc tggagtgaaa agcattttcg tctggaacac cggttgatgg
    73741 ctgggctgtt gataatgtca ttaagcatga tgccggtggg catggtcagc ggtctgcaac
    73801 aacttttcac cctgatttgt ctgttttata tcgggtcgat cattgccgag cctgcgcgtg
    73861 aaaccttaag tgctttgctg gcggacgcaa gagctcgcgg cagctatatg gggtttagcc
    73921 gtctgggtct ggcgattggc ggcgctattg gttatatcgg tggtggctgg ctgtttgacc
    73981 tgggcaaatc ggcgcaccag ccagagcttc cgtggatgat gctgggaatt attggcatct
    74041 tcactttcct tgcgctgggt tggcagttta gtcagaaacg cgcaacgcgt cgtttgctgg
    74101 aacgcgacgc ctgatttgat actcccccct gactcctcca tactgaaagt gaactgacag
    74161 ccaaatggag gagtcacgtg aagctataca tttacgatca ctgcccttac tgcctcaaag
    74221 cccgcatgat tttcggcctg aaaaatatcc ccgtcgaatt acatgttctg ctcaacgacg
    74281 acgcagaaac acccacccgg atggtcggtc aaaaacaggt tcccattctg caaaaagatg
    74341 acagccgcta tatgccagaa agcatggata tcgttcacta tgtcgataaa ctcgacggca
    74401 aaccgttact gaccggcaaa cgttcccctg ccattgaaga gtggctgcgc aaggtcaatg
    74461 gctacgccaa caaactgctg ttgccgcgtt ttgccaaatc ggcatttgat gagttttcta
    74521 ctcccgccgc gcgcaaatat ttcgtcgaca agaaagaggc cagcgcgggt aattttgccg
    74581 acctgctggc ccactctgac ggtctgatta agaatatcag cgatgatttg cgtgcgctgg
    74641 acaaactgat cgtcaaaccg aacgccgtga atggcgaact ttcggaagat gatattcagc
    74701 tatttccgct actgcgtaat ctgacgctgg tagccggaat caactggcca agccgcgttg
    74761 ctgattaccg cgataatatg gcgaaacaga cacaaatcaa tttgttatca tcaatggcga
    74821 tttaatcctg acccgccgcg gcagcctgta ttcgcgggtt gccgccgttt tcagtttttt
    74881 ctgctggcga gtttatcgct ggtgacacat gctgcgttgt gtcaggatat tgacgcagat
    74941 ttcatgtcga ggactaccat gaacaagttt ttatttgccg ctgcattgat cgtcagtggc
    75001 ctgctcgttg gctgtaatca actcacccaa tacaccatca ccgaacaaga aattaaccag
    75061 tcgcttgcga aacataataa tttctcaaaa gatatcggtt tacccggcgt ggctgacgcc
    75121 catattgttc tgacaaactt gatcagccaa attggtcgcg aagagccgaa taaggttacc
    75181 ctaaccggag acgccaatct ggacatgaac tccctgttcg gtagtcagaa agcgaccatg
    75241 aaactgaagc tgaaagcgct gcccgtgttt gataaagaaa aaggtgcgat cttcctgaaa
    75301 gagatggaag tggtcgatgc gacggtacaa ccggaaaaaa tgcaaacggt gatgcaaacg
    75361 ttgcttccct atttgaacca ggcattacgc aattacttta accagcaacc tgcttacgtc
    75421 ctgcgcgaag atggcagtca gggcgaagca atggcgaaaa aactggcgaa aggcattgaa
    75481 gtgaagccag gcgaaattgt cattccgttt acggattaat gacgagggcg cattcgcgcc
    75541 ctttattttt cgtgcaaagg aaaacgtttc cgcttatcct ttgtgtccgg caaaaacatt
    75601 ccttcagccg gagcatagag attaatgact gcaccatccc aggtattaaa gatccgccgc
    75661 ccagacgact ggcaccttca cctccgcgat ggcgacatgt taaaaactgt cgtgccgtat
    75721 accagcgaaa tttatggacg ggctatcgta atgcccaatc tggctccgcc cgtgaccaca
    75781 gttgaggctg ccgtggcgta tcgccaacgt attcttgacg ccgtacctgc cgggcacgat
    75841 ttcaccccat tgatgacctg ttatttaaca gattcgctgg atcctaatga gctggagcgc
    75901 ggatttaatg aaggcgtgtt caccgctgca aaactttacc cggcaaacgc aaccactaac
    75961 tccagccacg gcgtgacgtc aattgacgca atcatgccgg tacttgagcg catggaaaaa
    76021 atcggtatgc cgctactggt gcatggtgaa gtgacacatg cagatatcga catttttgat
    76081 cgtgaagcgc gctttataga aagcgtgatg gaacctctgc gccagcgcct gactgcgctg
    76141 aaagtcgttt ttgagcacat caccaccaaa gatgctgccg actatgtccg tgacggaaat
    76201 gaacggctgg ctgccaccat cactccgcag catctgatgt ttaaccgcaa ccatatgctg
    76261 gttggtggcg tgcgtccgca cctgtattgt ttacccatcc tcaaacgcaa tattcaccaa
    76321 caggcattgc gtgaactggt cgccagcggt tttaatcgag tattcctcgg tacggattct
    76381 gcgccacatg cacgtcatcg caaagagagc agttgcggct gcgcgggctg cttcaacgcc
    76441 ccaaccgcgc tgggcagtta cgctaccgtc tttgaagaga tgaatgctct gcagcacttt
    76501 gaagcattct gttctgtaaa cggcccgcag ttctatgggt tgccggtcaa cgacacattc
    76561 atcgaactgg tacgtgaaga gcaacaggtt gctgaaagca tcgcactgac tgatgacacc
    76621 ctggtgccat tcctcgccgg ggaaacggta cgctggtccg ttaaacaata aaaatcgtag
    76681 cttcctgttg tcattaggtt atttaacctg tataaataac cagtatattc aacagggggc
    76741 tattatgcga attgaagtca ccatagcgaa aacttctcca ttgccagctg gggctattga
    76801 cgccctggct ggcgaacttt cccgccgtat tcagtatgcg tttcctgata atgaaggcca
    76861 cgtatcggta cgttatgccg cagcgaataa tttatcggtt attggcgcaa caaaagaaga
    76921 taaacagcgc attagcgaaa ttctccagga aacgtgggaa agcgccgatg actggtttgt
    76981 cagcgaataa tatgcagtga tttttgtttt tgccgggtcg ccccggcttt tttattttac
    77041 ttacgaattt tgagtacaaa atttctacac ttcctcaaaa taattgcaca atatgctaaa
    77101 aaaatatgaa tgacatgctg ttttattgtt catataaatc ttaaaagcag aaatttatct
    77161 gttcccccct ttaaaaattc ggtgaatacc cttacttatt ggtatactga agttgcttca
    77221 gataaaaacg cattgaacct cgaataacgt tgtctagtaa cacgaattag ggggccatga
    77281 tggaaaaaaa taatgaagtc attcagactc atccgctcgt agggtgggac atcagcaccg
    77341 ttgatagcta tgatgcgctg atgttgcgtt tgcactacca gaccccaaat aagtccgagc
    77401 aggaagggac tgaagttggt caaacgctct ggttaaccac tgatgttgcc agacaattta
    77461 tttcgatatt agaagcagga atcgccaaaa ttgaatccgg tgatttccag gtaaacgagt
    77521 atcggcgtca ttaacatgac atgaccatcc acccaaaagg cacctcaccg gtgcctttac
    77581 catttctggg ttgtatatct gcattcagtt aattactctg cttaaaccat gagagcagag
    77641 agaaaaaaat gaaatacgat ctcatcatta ttggcagcgg ttccgtaggc gctgccgccg
    77701 ggtattatgc aacccgcgcc ggtttaaacg tgctaatgac cgacgcccat atgccaccgc
    77761 atcaacacgg cagccaccac ggcgatacgc gattaattcg ccatgcttat ggtgaaggcg
    77821 aaaagtatgt cccgctggtc ctccgcgcgc aaacgctgtg ggatgaactt tcccgccaca
    77881 acgaagatga tcccattttt gtacgctctg gtgtcattaa cctcggcccg gctgactccg
    77941 catttctcgc caacgtcgcc cacagcgccg aacagtggca actcaacgtt gaaaagctcg
    78001 atgcgcaagg gattatggcc cgctggccag aaatacgcgt cccggacaac tacatcggct
    78061 tatttgagac tgattccggt tttttgcgca gcgaactggc gattaaaacc tggatccaac
    78121 tggcgaagga agcgggctgt gcgcaactgt tcaactgccc ggtcaccgca attcgtcatg
    78181 acgatgatgg cgtaactatt gaaacggttg acggtgagta tcaggcgaaa aaagcgattg
    78241 tctgcgcggg aacatgggta aaagacctgc tcccggagct gcctgtccag cccgtacgta
    78301 aagtatttgc ctggtatcag gccgatggcc gctatagcgt gaagaataaa ttcccggcgt
    78361 ttaccggtga actgcccaat ggcgatcaat attatggttt tccggcagaa aacgacgcgt
    78421 tgaagattgg caaacataac ggaggccagg ttatccattc agcggatgaa cgtgttccgt
    78481 ttgcggaagt ggtcagcgat ggttcggaag ccttcccgtt cttgcgcaat gtattgccgg
    78541 gtatcggttg ctgcctgtac ggcgctgcct gcacctatga taattcgcct gacgaagatt
    78601 ttattatcga taccctacc
//
LOCUS       DAJVQG010000003        74284 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJVQG010000003 DAJVQG010000000
VERSION     DAJVQG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 74284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74284
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.003"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            1..943
                     /locus_tag="OQM83_000168"
                     /pseudo
     CDS             1..943
                     /locus_tag="OQM83_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
     gene            979..1665
                     /gene="mlrA"
                     /locus_tag="OQM83_000169"
     CDS             979..1665
                     /gene="mlrA"
                     /locus_tag="OQM83_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HCS1518538.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1725..1832
                     /locus_tag="OQM83_000170"
     CDS             1725..1832
                     /locus_tag="OQM83_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCS1518539.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1813..2544)
                     /gene="yehW"
                     /locus_tag="OQM83_000171"
     CDS             complement(1813..2544)
                     /gene="yehW"
                     /locus_tag="OQM83_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCS1518540.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2549..3475)
                     /gene="yehX"
                     /locus_tag="OQM83_000172"
     CDS             complement(2549..3475)
                     /gene="yehX"
                     /locus_tag="OQM83_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCS1518541.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3468..4625)
                     /gene="yehY"
                     /locus_tag="OQM83_000173"
     CDS             complement(3468..4625)
                     /gene="yehY"
                     /locus_tag="OQM83_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCS1518542.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(4632..5549)
                     /gene="osmF"
                     /locus_tag="OQM83_000174"
     CDS             complement(4632..5549)
                     /gene="osmF"
                     /locus_tag="OQM83_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCS1518543.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5801..8098)
                     /gene="bglX"
                     /locus_tag="OQM83_000175"
     CDS             complement(5801..8098)
                     /gene="bglX"
                     /locus_tag="OQM83_000175"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCS1518544.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLITALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8294..10009
                     /gene="dld"
                     /locus_tag="OQM83_000176"
     CDS             8294..10009
                     /gene="dld"
                     /locus_tag="OQM83_000176"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCS1518545.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10048..10980)
                     /gene="pbpG"
                     /locus_tag="OQM83_000177"
     CDS             complement(10048..10980)
                     /gene="pbpG"
                     /locus_tag="OQM83_000177"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCS1518546.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11154..11741)
                     /locus_tag="OQM83_000178"
     CDS             complement(11154..11741)
                     /locus_tag="OQM83_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCS1518547.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11911..12489
                     /locus_tag="OQM83_000179"
     CDS             11911..12489
                     /locus_tag="OQM83_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1518548.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12619..13380)
                     /locus_tag="OQM83_000180"
     CDS             complement(12619..13380)
                     /locus_tag="OQM83_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1518549.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13433..14869)
                     /gene="mdtQ"
                     /locus_tag="OQM83_000181"
     CDS             complement(13433..14869)
                     /gene="mdtQ"
                     /locus_tag="OQM83_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCS1518550.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIVGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            15093..15176
                     /gene="yohP"
                     /locus_tag="OQM83_000182"
     CDS             15093..15176
                     /gene="yohP"
                     /locus_tag="OQM83_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCS1518551.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(15549..16496)
                     /gene="dusC"
                     /locus_tag="OQM83_000183"
     CDS             complement(15549..16496)
                     /gene="dusC"
                     /locus_tag="OQM83_000183"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCS1518552.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            16735..17133
                     /locus_tag="OQM83_000184"
     CDS             16735..17133
                     /locus_tag="OQM83_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCS1518553.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            17130..17825
                     /locus_tag="OQM83_000185"
     CDS             17130..17825
                     /locus_tag="OQM83_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCS1518554.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            17955..18839
                     /gene="cdd"
                     /locus_tag="OQM83_000186"
     CDS             17955..18839
                     /gene="cdd"
                     /locus_tag="OQM83_000186"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCS1518555.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            18989..19708
                     /gene="sanA"
                     /locus_tag="OQM83_000187"
     CDS             18989..19708
                     /gene="sanA"
                     /locus_tag="OQM83_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCS1518556.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            19711..19950
                     /locus_tag="OQM83_000188"
     CDS             19711..19950
                     /locus_tag="OQM83_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCS1518557.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            20144..21382
                     /gene="preT"
                     /locus_tag="OQM83_000189"
                     /pseudo
     CDS             20144..21382
                     /gene="preT"
                     /locus_tag="OQM83_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            21376..22611
                     /gene="preA"
                     /locus_tag="OQM83_000190"
     CDS             21376..22611
                     /gene="preA"
                     /locus_tag="OQM83_000190"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCS1518558.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(22738..23748)
                     /gene="mglC"
                     /locus_tag="OQM83_000191"
     CDS             complement(22738..23748)
                     /gene="mglC"
                     /locus_tag="OQM83_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCS1518559.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(23764..25284)
                     /gene="mglA"
                     /locus_tag="OQM83_000192"
     CDS             complement(23764..25284)
                     /gene="mglA"
                     /locus_tag="OQM83_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCS1518560.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(25345..26343)
                     /gene="mglB"
                     /locus_tag="OQM83_000193"
     CDS             complement(25345..26343)
                     /gene="mglB"
                     /locus_tag="OQM83_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCS1518561.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(26623..27663)
                     /gene="galS"
                     /locus_tag="OQM83_000194"
                     /pseudo
     CDS             complement(26623..27663)
                     /gene="galS"
                     /locus_tag="OQM83_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
     gene            complement(27805..28962)
                     /locus_tag="OQM83_000195"
     CDS             complement(27805..28962)
                     /locus_tag="OQM83_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCS1518562.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(28979..29647)
                     /gene="folE"
                     /locus_tag="OQM83_000196"
     CDS             complement(28979..29647)
                     /gene="folE"
                     /locus_tag="OQM83_000196"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCS1518563.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            29905..30741
                     /gene="yeiG"
                     /locus_tag="OQM83_000197"
     CDS             29905..30741
                     /gene="yeiG"
                     /locus_tag="OQM83_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCS1518564.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(30773..32764)
                     /gene="cirA"
                     /locus_tag="OQM83_000198"
     CDS             complement(30773..32764)
                     /gene="cirA"
                     /locus_tag="OQM83_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HCS1518565.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(33056..34525)
                     /gene="lysP"
                     /locus_tag="OQM83_000199"
     CDS             complement(33056..34525)
                     /gene="lysP"
                     /locus_tag="OQM83_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCS1518566.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(34730..35601)
                     /locus_tag="OQM83_000200"
                     /pseudo
     CDS             complement(34730..35601)
                     /locus_tag="OQM83_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            35700..36749
                     /locus_tag="OQM83_000201"
     CDS             35700..36749
                     /locus_tag="OQM83_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCS1518567.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            36823..37680
                     /gene="nfo"
                     /locus_tag="OQM83_000202"
     CDS             36823..37680
                     /gene="nfo"
                     /locus_tag="OQM83_000202"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCS1518568.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAVEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKFRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            37684..38772
                     /locus_tag="OQM83_000203"
     CDS             37684..38772
                     /locus_tag="OQM83_000203"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS1518569.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(38828..40078)
                     /locus_tag="OQM83_000204"
     CDS             complement(38828..40078)
                     /locus_tag="OQM83_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCS1518570.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(40178..41119)
                     /gene="rihB"
                     /locus_tag="OQM83_000205"
     CDS             complement(40178..41119)
                     /gene="rihB"
                     /locus_tag="OQM83_000205"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCS1518571.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            41249..41947
                     /gene="yeiL"
                     /locus_tag="OQM83_000206"
     CDS             41249..41947
                     /gene="yeiL"
                     /locus_tag="OQM83_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HCS1518572.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(42014..43264)
                     /locus_tag="OQM83_000207"
     CDS             complement(42014..43264)
                     /locus_tag="OQM83_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCS1518573.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(43358..44296)
                     /gene="psuG"
                     /locus_tag="OQM83_000208"
     CDS             complement(43358..44296)
                     /gene="psuG"
                     /locus_tag="OQM83_000208"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCS1518574.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(44284..45225)
                     /locus_tag="OQM83_000209"
     CDS             complement(44284..45225)
                     /locus_tag="OQM83_000209"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCS1518575.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEVLVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(45683..47374)
                     /gene="fruA"
                     /locus_tag="OQM83_000210"
     CDS             complement(45683..47374)
                     /gene="fruA"
                     /locus_tag="OQM83_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCS1518576.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(47391..48329)
                     /gene="fruK"
                     /locus_tag="OQM83_000211"
     CDS             complement(47391..48329)
                     /gene="fruK"
                     /locus_tag="OQM83_000211"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCS1518577.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(48329..49459)
                     /gene="fruB"
                     /locus_tag="OQM83_000212"
     CDS             complement(48329..49459)
                     /gene="fruB"
                     /locus_tag="OQM83_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCS1518578.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            49827..51008
                     /gene="setB"
                     /locus_tag="OQM83_000213"
     CDS             49827..51008
                     /gene="setB"
                     /locus_tag="OQM83_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCS1518579.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(51005..51259)
                     /locus_tag="OQM83_000214"
     CDS             complement(51005..51259)
                     /locus_tag="OQM83_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCS1518580.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            51414..51986
                     /gene="yeiP"
                     /locus_tag="OQM83_000215"
     CDS             51414..51986
                     /gene="yeiP"
                     /locus_tag="OQM83_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCS1518581.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            52209..53675
                     /locus_tag="OQM83_000216"
     CDS             52209..53675
                     /locus_tag="OQM83_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCS1518582.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKKHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            53793..54779
                     /gene="yeiR"
                     /locus_tag="OQM83_000217"
     CDS             53793..54779
                     /gene="yeiR"
                     /locus_tag="OQM83_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCS1518583.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            54818..55531
                     /gene="lpxT"
                     /locus_tag="OQM83_000218"
     CDS             54818..55531
                     /gene="lpxT"
                     /locus_tag="OQM83_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCS1518584.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            55943..56509
                     /gene="mepS"
                     /locus_tag="OQM83_000219"
     CDS             55943..56509
                     /gene="mepS"
                     /locus_tag="OQM83_000219"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCS1518585.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            56690..58246
                     /locus_tag="OQM83_000220"
     CDS             56690..58246
                     /locus_tag="OQM83_000220"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCS1518586.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALNINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            58328..60142
                     /locus_tag="OQM83_000221"
     CDS             58328..60142
                     /locus_tag="OQM83_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1518587.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            60143..61237
                     /locus_tag="OQM83_000222"
     CDS             60143..61237
                     /locus_tag="OQM83_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCS1518588.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            61237..62262
                     /locus_tag="OQM83_000223"
     CDS             61237..62262
                     /locus_tag="OQM83_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1518589.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            62264..63853
                     /gene="yejF"
                     /locus_tag="OQM83_000224"
     CDS             62264..63853
                     /gene="yejF"
                     /locus_tag="OQM83_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCS1518590.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(63857..64201)
                     /locus_tag="OQM83_000225"
     CDS             complement(63857..64201)
                     /locus_tag="OQM83_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1518591.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(64534..65654)
                     /locus_tag="OQM83_000226"
                     /pseudo
     CDS             complement(64534..65654)
                     /locus_tag="OQM83_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(65682..66377)
                     /gene="rsuA"
                     /locus_tag="OQM83_000227"
     CDS             complement(65682..66377)
                     /gene="rsuA"
                     /locus_tag="OQM83_000227"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCS1518592.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            66527..68287
                     /locus_tag="OQM83_000228"
     CDS             66527..68287
                     /locus_tag="OQM83_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCS1518593.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            68412..68696
                     /gene="rplY"
                     /locus_tag="OQM83_000229"
     CDS             68412..68696
                     /gene="rplY"
                     /locus_tag="OQM83_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCS1518594.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(68835..69842)
                     /gene="yejK"
                     /locus_tag="OQM83_000230"
     CDS             complement(68835..69842)
                     /gene="yejK"
                     /locus_tag="OQM83_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCS1518595.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            70024..70251
                     /locus_tag="OQM83_000231"
     CDS             70024..70251
                     /locus_tag="OQM83_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCS1518596.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            70271..72031
                     /gene="pbgA"
                     /locus_tag="OQM83_000232"
     CDS             70271..72031
                     /gene="pbgA"
                     /locus_tag="OQM83_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCS1518597.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            72106..72182
                     /locus_tag="OQM83_000233"
     tRNA            72106..72182
                     /locus_tag="OQM83_000233"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72140..72142,aa:Pro,seq:ggg)
     gene            complement(72285..74284)
                     /gene="yejO"
                     /locus_tag="OQM83_000234"
                     /pseudo
     CDS             complement(72285..74284)
                     /gene="yejO"
                     /locus_tag="OQM83_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT        18235 a        19573 c        18241 g        18235 t
ORIGIN      
        1 gccaatgtta ggtgaaaaaa gactggtttc atctgtaaca aaagaggatt tgcttttcgt
       61 aaggagagat ctgttgaccg gttatcaaaa gctttctaac ggaaaaattt cttccataaa
      121 aggacgctca gtggtcacag taaactacta tatgacaacc atagctggaa tgtttcaatt
      181 tgcaacagat aatggttata catcaggaaa tccatttaac ggtctgacac cattaaaaaa
      241 gtccaagata gaaccagatc ccctcacccg tgacgaattt attcgtttta ttgaggcttg
      301 ccgtcatcaa caaacaaaaa acctgtggat tatcgctgta tacacgggta ttcgtcacgg
      361 ggagctggta tcactggcct gggaagatat agaccttaaa gcgaggacta taaccatccg
      421 ccggaattat acaaaactag gcgaattcac tccaccaaaa actgatgctg gtaccggaag
      481 aacgattcat ctagttcaac cagctattga tgctcttaaa agccaagcgg aaatgaccat
      541 gcttggaaaa cagcattctg tagaggtaaa gcagagggaa tatgggagaa gtactgtgca
      601 taaatgcact tttgttttta gtcctcaggt aataaaatag cggcagtttt ccggaccgca
      661 ctataaggtt gactccatca gggagtcatg gacaagtatc ttaaaacgcg caggtctgag
      721 acacagaaaa tcgtaccaat ccaggcatac ttatgcatgc tggtcacttg ccgctggagc
      781 taatcctagt tttatcgcaa gccagatggg ccacacaaac gcacagatgg tattcaatgt
      841 ttacggagca tggatgaaag acaacaatca cgaacagata gaactcctta acaaaagact
      901 atctgaaagt gtcccatgta tgccccataa gaaagtgggg taaaataaaa acttgtaaaa
      961 tcagttagtt tacccttaat ccctgtcacg ttacgcgcgt ggcagaggcg ttacgggttg
     1021 ctgaaaccgc aacggacaga cggcggtcat cgactgttca acgatgccga tattgaccgg
     1081 atccgcgaga tcaaacgctg gatcgacaac ggcgtgcagg tcagcaaagt taaaatgctg
     1141 ctcagtaatg aaaatgttga tgtgcagaac ggctggcgcg atcagcaaga aacattactg
     1201 acctacctgc aaagcggcaa tctgcatagc ctgcgaacgt ggatcaaaga gcgcggtcag
     1261 gattaccccg cccagacact caccacacat ctgtttattc ctctgcgccg acggcttcag
     1321 tgccaacaac cgactctcca ggcgctgctg gcgatcctcg acggcgtact gatcaactac
     1381 atcgccattt gtctggcttc ggcacgtaaa aaacagggta aagatgcgct ggtggttggc
     1441 tggaatattc aggataccac ccgtctgtgg ctggagggct ggattgccag tcaacaagga
     1501 tggcgcattg atgtcctcgc ccactcgctc aatcaactac gccctgaact attcgaaggc
     1561 cgtacattgc tggtgtggtg cggtgaaaat cgaacctccg cccaacagca gcaactcacc
     1621 agttggcaag aacaaggcca tgatattttc ccactcggca tttaatgatt cgttaacaaa
     1681 tgcgctttac tgtacaatcc tttcgttaac ataaggagtg caatatgcgc atagctaaaa
     1741 ttggggtcat cgccctgttc ctgtttatgg cgttaggcgg aattggtggc gtcatgctcg
     1801 caggttatac ctttattttg cgtgctggct aagcgcctgc accagccttt caaacaggcg
     1861 gtctgcgatg atcgccgcca gtgccaccag taacgccccc tggatcacat acgcggtatt
     1921 aaatccgcta agcccgatga tgatgggcgt acccagcgtg ctggccccta ccgttgaggc
     1981 gatcgtcgcc gtaccaatgt tgataatcac cgaagttcgc acgcccgcca gaatcaccgg
     2041 agccgccagc ggtagctcga ccttacgcag tcgctgacca cgactcattc ccataccttt
     2101 cgcaacttct gtcacgctgg catcaatcgc tcccagcccg gcaagtgtcg cctgcaggac
     2161 gggcagcaca ccgtaaagga tcaaggcgat aatcgctggt tgcagaccaa agccgatcac
     2221 cggaacggca atcgccagca ctgcgacagg cggaaaagtc tgtccaacgg cggcaatagt
     2281 ttccaccagt gggcgaaatt ccgcgcccca cgggcgagtg acagcaattc cggcaccagt
     2341 gccaatgatc accgcaaaca aactcgaaat tcccaccagc cagaaatgag ccagtgccag
     2401 agctgcaaaa ctttcttgct gataaacggg tcgtggcagt tgtgggaaca aggcagcaaa
     2461 cagcggctgg ctgtaaggca gccagaaaat cagtgccaca aacaaagcaa tgagccagaa
     2521 cagcggatcg cgcaacatct tcatacgctt acgcctcctc cagcagatcc tgaaaatgca
     2581 gcgtgccgca aggctggccc tgcgtgttca ccaccggcag cacctcgcat ccccgcgcga
     2641 caaacaggga gagcgcatcg cgtagcgtca tctcttctgc cagtgcctca ccttctgccc
     2701 gttcttcgcg acgcacgtaa tccgccacac tacgtaacga aagcaggcgc acacccagtt
     2761 cgctgcgccc aaaaaactgg cggacaaaat cgtttgccgg acgagtcagc atcgtcagcg
     2821 gattgccctg ctgcaccact tcaccgtgat ccatcaatac cagatgttct gccagccgta
     2881 gcgcctcatc aatatcatga gtgaccagca caatggtacg ccccagcaaa cggtgaatgc
     2941 gcgtcatctc ttgttgcaac gcgccgcgcg ttaccgggtc cagtgcgcca aaaggttcat
     3001 ccatcagtaa gacttgcgga tcggcagcca gtgcgcgcgc cactcccaca cgttgctgct
     3061 gaccaccgga aagctgatgc ggataacgct cacgtaaatt tgactccagc cccagtagcg
     3121 ccattaattc gtcgatacga tcgtcaatcc tcgcccgcga ccatttttgt aattgcggca
     3181 cggtagcgat gttttgcgcc acgctccagt gggggaacag gccaatagat tgaatggcat
     3241 agcccatccg gcggcgcaac tccagtactg gcagcgagcg aatttcttct ccggcaaagc
     3301 ggatcactcc gctgtcatgc tccaccaggc ggttaatcat tttcagggtg gtggatttgc
     3361 cggagccaga tgtgccaatc agcaccgaaa aactcccttc ctgaaaattg agattgagat
     3421 cgttaacggc tttttgtgcg ccgaacagtt tgctgacatg gccaaattca atcattacgt
     3481 ttcaccttca gcagtgcgat aagcaaatcg aacagcgcgt cggtaagcac cgccagaaca
     3541 attaccggga tcacccccag caacactaaa tcaatggcgc tgcttagcag cccctggaaa
     3601 accagcgcac caaaaccgcc tgcgccgatt aacgccgcaa tcaccgccat acctacagtt
     3661 tgcaccatca ccacccgcag gctgcgcaga aataccggta acgccagtgg taactgaaca
     3721 tgcaggaatc gctgcgcccc gctcatgccc atcgctctgg cgctctccag cacatcgcgc
     3781 gggatctggt tcaaaccgac taccacgccg cgcaccagcg gcagcaaggc atagagcacc
     3841 agcgcaatca gtgcgggtgt cattccggtt cctgctatgc cgagcgtccc cagccacgga
     3901 aaggccgtaa ccagcgcggc aagcggcgca atcaacaggc caaagagcgc caccgaaggc
     3961 acggtctgaa tgacattgag cagagaaaaa attgccccct gccgagcagt ggaaaagtag
     4021 caccagatgc ccaacggcac accaatcact aacgcaggca gcaccgcacc aaacagcaac
     4081 gtcagatgtt gtgccagcgc gtcgtcaaac acatcctgac ggttggcgta ttctttcatt
     4141 agtgagagat cgttaagcgt gccggagtac agcaaccaca gcggaataat ggcaatctgc
     4201 atatgcaaca accagcgcca cagcggatgc gtggagattc ggcggatggc atcactacag
     4261 gccagcaatg ccagcgccgc ggccagccag aaaccactgc cgaggctggt acgcgccagc
     4321 gcactgccat tttgcgccag ttgggtcgcc gcctttcctg ctccccacac cagcaatacg
     4381 aagacgaatt gcgccagaat gagtgcaaaa atgctgcctt ttttagcggg aataaaacag
     4441 gctgtcagcc aggcgcaacc aacgcccacc agcatccaga tcgtttgcgg ccacagttgc
     4501 cagagatgac gcccttcacc cgagactaaa cgattaggcg cgtaactgat aaacggcagc
     4561 gccgctgcga ttgccgtcag caacagcagc agcgccagaa caggattaat acgtagataa
     4621 gtcacgggaa attacttcgt ccaccctttt tgtttcaggt agtcggcagc cacttttttg
     4681 gcatccagtc cttccactgc aatgctggca ttcagttgct gcaatgtttt tgcatcgagg
     4741 ctggcgaaga ctggctgtag ccactgtgcc atttgcggat actctttcag caccgactca
     4801 cgcaccactg gtgcaggcgc gtagataggt tgcacgcctt tcggatcgct taaggtttgc
     4861 agccccagcg ccgcgaccgg accgtcagtg ccgtaagcca ttgcggcatt aacgccagaa
     4921 gtttgctggg cagcggcttt aatcgttacc gccgtgtcgc cgccagccag tgacagtaac
     4981 tgatcctgat cgagtttaaa gtcgtaggct ttttcaaacg cgggtaacgc atcggcgcgt
     5041 tcgataaact ctgccgaggc tgccagtttg aaggtgccgc cctctttcag ataacgactc
     5101 aggtcggcaa gcgaagtgag tttgtttttc tctgccacat cctgacgcac ggcgatggtc
     5161 caggtgttat tcgctggtgc gggcgtcagc cagattaact tgttttgctc tgcatcgagt
     5221 tttttgactt tctcgtaacc ttgctgcgcg tttttccatg ccgcatcgtt ttcatcttta
     5281 aagaagaaag cgccattacc ggtatattcc ggatagatat ccagttcacc ggaagtaatc
     5341 gccccgcgca ccacaggagt cgttccgagt tgcactttat tcaccgtcgg tacgccgtgg
     5401 ctttccagca cctgcaagat gatgttgccg agtagcgcgc cttcggtatc gatttttgaa
     5461 ccgactttaa cgggggaagc cgcctgtagc ggcaggctca cggctgccaa cataaccagt
     5521 gaacctgccc agacctttga gagtggcatg atgctttcct cattctttta ctgttgtttt
     5581 cagcgaatta agagaaaagc atagttgata atgacagagt tagcctgagc ggaaggattc
     5641 agttgcagaa tcagataaat acttaaaggg catattttgc ccggcgtttc ttgccggatg
     5701 cggcgtaaac gccttatccg gcctacaaag gtcgaaaatt cagcaagtta agatatgaac
     5761 atcaggcaaa gggcgcaaac gtcgcgccct tactaaagca ttacagcaac tcaaactcgc
     5821 ctttcttaac gcgtgcggaa tcagtgccga taaagacatt gaacttgcca ggttcggcgt
     5881 catatttcat ctgttgattc cagaacttca gcgcctcgat atcaatcggg aagctgacag
     5941 tctgagtttc gcccggtttc agggtgattt tctcaaagcc tttcagctgc ttcactggac
     6001 ggctcatgga agccgtcaca tcctgcaagt acatctgtac taccgtggcc ccttcgcgtt
     6061 taccggtgtt cgtcacctgc acgctggcgg tcactttgcc gtcacgcttc atggtcggcg
     6121 cagaaagttt cacatcagag acggtgaaag tggtatagct cagaccatag ccgaacggat
     6181 aaagcgcccc gttagcttca tcaaaataac gcgaagtgta tttgttcggc ttgtcggcat
     6241 tatacggacg accggtattc agatggctgt agtacaccgg gatctgcccg acagaacgcg
     6301 ggaaggacat tggcagcttg ccggacgggt tgtaatcgcc aaacaataca tcggcaattg
     6361 cattaccgcc ttcagtcccc gcaaaccagg tttccagaat cgcatcagcc tgctgatctt
     6421 ctttcaccag cgccagcgga cgcccgttca tcagcaccag caccagcggt ttaccggtgg
     6481 ctttcagcgc cgtaatcaag tcacgttggc tttgcggaat agtgatatcg gtacggctgg
     6541 aggcctcgtg cgccatcccc tgtgcttcac cgaccacagc caccaccaca tcagattgtt
     6601 tcgccgtctg caccgcttca tcaatcatct cttgcggcga gcgcggatcg actttgaccg
     6661 cttcttcata ctgattcagg aaatcgataa tgcctttgtc actggtaacg ttcgcccctt
     6721 tggcatacag cactttaccg ttttcaccga cggcattttt aatcccggtc agtacggtca
     6781 cggattgatc ggcaacaccg gctgcggacc agctgcccat cacgtcacgt ttactgtccg
     6841 ccagtggccc aaccaccgca atggtggccg attttttcag cggtaacgtt tcgagacggt
     6901 ttttcagcaa caccaggctt tcgcgcgcca cttcacgcgc ttctttacgg tgcaggcggc
     6961 tttcggcatt ggtatccacc gggtcagact ctttcggccc caaatggctg tatgggtcgt
     7021 taaacaatcc catatcatat ttaacgttca gtacatggcg ggcagcatcg tccagctctt
     7081 ccatcgtcac tttgccggat ttgatcaacc caggcagata cttcgagtaa tactcgtcgc
     7141 tcatactcat gttgattccg gatttcagcg ccacgcgcac cgcatcttcc gggtctgccg
     7201 ccgtgccatg tttaatcagc tctttgattg caccgtgatc ggaaacggtg atgcctttaa
     7261 agccccactg gtcgcgcaga acatctttca gcagccagga atcggaggtg gctggcgtgc
     7321 cgttcagcga gttcagcgcc accatcaccg cgccgctgcc tgcgtccagc cccgctttgt
     7381 acggcggcat ataatcatta aacaggcgct gcggactcat atcgacggtg ttgtactctt
     7441 taccgccttc taccgcgccg tatgcggcaa agtgtttgac gctggtcatc accgagtagc
     7501 gatctgccgg gcttttaccc tgcatcgctt ccaccatggt tttacccatt gttgaggtga
     7561 gatacgtatc ttcgccaaaa ccttcggaag cacgtcccca gcgcggatcg cgcgagacat
     7621 cgaccatcgg tgcccaggtc atattcaggc catcatctgc cgcttcataa gcagagacac
     7681 gtccgaccgt tttcactgca tcgaggttaa aagacgaggc cagaccgagg ctaatcggga
     7741 acaccgtgcg ctgaccgtgc agcacgtcgt aagcaaagaa aagaggaatt ttcaggcggc
     7801 ttaattccat cacctgatcc tgcatggcgc ggatatcctg acgggttacg gtgttgaaaa
     7861 tcgccccaac ctgaccgtct ttgatcatct cgcggatcgc ctctttcggg ttatccggac
     7921 cgacgctgat taagcgcagc tgaccaattt tctcatcaac tgtcattttc ttaagcagtt
     7981 cggtgacgaa cgcatcccgc gcttcgggcg ttaatggatg gttgccgaat aaatcatccg
     8041 ccagtgctgg ctgcagggcc agactcaccg cgattcctac tgaacatagc catttcatat
     8101 ggatttttct tcctctgttg cccgacgagg cagcgaaaat ggtgcaaaaa ccgtagtttg
     8161 ccataagcat gatggagaga gaaaaagaat gctcagttta ttgtctgaat tttcaaaata
     8221 ttcactcgct gaattgtcat acaaggcgct attctagttt gtgatatttt ttcgccacca
     8281 caaggagtgg aaaatgtctt ccatgacaac aactgataat aaagcctttt tgaatgaact
     8341 tgcccgtctg gtcggtcatt cacacctgct caccgatccc gcaaaaacgg cccgctatcg
     8401 caagggcttc cgttctggtc agggcgacgc gctggctgtc gttttccctg gctcactact
     8461 ggaattgtgg cgggtgctga aagcctgtgt caccgccgac aaaattattc tgatgcaggc
     8521 tgccaataca ggcctgaccg aaggatcgac gccaaacggt aacgactatg atcgcgatat
     8581 cgtgatcatc agcaccctgc gtctcgacaa gctgcacgtt cttggcaagg gcgaacaggt
     8641 gctggcctat ccgggcacca cgctctattc gctggaaaaa gccctcaaac cgctgggacg
     8701 cgaaccgcac tcagtgattg gatcatcgtg tataggcgca tcggtcatcg gcggtatttg
     8761 taacaactcc ggcggctcgc tggtgcaacg tggcccggcg tataccgaaa tgtcgttatt
     8821 cgcgcgtata aatgaagacg gcaaactgac gctggtgaac catctgggga ttgatctggg
     8881 cgaaacgccg gagcagatcc ttagcaagct ggatgatgat cgcatcaaag atgacgatgt
     8941 gcgtcacgat ggtcgtcacg cccacgatta tgactatgtc caccgcgttc gtgatattga
     9001 agccgacacg cccgcacgtt ataacgccga tcctgatcgg ttatttgaat cttctggttg
     9061 cgccgggaag ctggcggtct ttgctgtacg tctagatacc ttcgaagcgg aaaaaaatca
     9121 gcaggtgttt tatatcggca ccaaccagcc ggaagtgctg accgaaatcc gccgtcatat
     9181 tctggctaac ttcgaaaatc tgccggttgc cggggaatat atacaccggg atatctacga
     9241 tattgcggaa aaatacggca aagacacctt cctgatgatt gataagttag gcaccgacaa
     9301 gatgccgttc ttctttaatc tcaagggacg caccgatgcg atgctggaga aagtgaaatt
     9361 cttccgtccg cattttactg accgtgcgat gcaaaaattc ggtcacctgt tccccagcca
     9421 tttaccgccg cgcatgaaaa actggcgcga taaatacgag catcatctgc tgttaaaaat
     9481 ggcgggcgat ggcgtgggcg aagccaaatc gtggctggtg gattatttca aacaggccga
     9541 aggcgatttc tttgtctgta cgccggagga aggcagcaaa gcgtttttac accgtttcgc
     9601 cgctgcgggc gcagcaattc gttatcaggc ggtgcattcc gatgaagtcg aagacattct
     9661 ggcgttggat atcgctctgc ggcgtaacga caccgagtgg tatgagcatt taccgccgga
     9721 gatcgacagc cagctggtgc acaagctcta ttacggccat tttatgtgct atgtcttcca
     9781 tcaggattac atagtgaaaa aaggcgtgga tgtgcatgcg ttgaaagaac agatgctgga
     9841 actgctacag cagcgtggcg cgcaataccc tgccgagcat aacgtcggtc atttgtataa
     9901 agcaccggag acgttgcaga agttttatcg cgagaacgat ccgaccaaca gtatgaatcc
     9961 ggggatcggt aaaaccagta agcggaaaaa ctggcaggaa gtggagtaaa aattacggat
    10021 ggcatagtat cgccatccgt cattcactta atcgttctgt gccgtctgcc ccgccgccgc
    10081 catttgggcg gctttttgtt ttttatagct caacgctgct gctggaacag gcatcacttt
    10141 accagtttca atccaggtac gcaggcggct ggcatcggca aaatgggtat atttgccaaa
    10201 cgcatccatc actaccagcg ctaccggttt attattgata accgtacgca tcaccagaca
    10261 atggcccgcc gcattggtaa aaccggtttt ggttaactga atattccagt tatcgcgata
    10321 caccagatgg ttagtattgc ggaacggcag cgtatacgtc gggttagaga aggttgccat
    10381 atcttcccgg gtagtactta actgcccgat caacggatat tgtttgctgg caatgagcag
    10441 tttggttaag tcacgggcag ttgaaacgtt atgcaccgac aatccggtag gttcaacaaa
    10501 gcgcgtgttg ttcattccga gcgatttcgc tttcgcattc attgccttaa taaaggcttt
    10561 gtaaccaccg ggataatggt gtgcaaggct tgccgccgcg cggttttctg aagacatcag
    10621 cgccagcaac agcatatctt tacggctgat ttcgctattc agtcgtacgc gcgaatagac
    10681 ccctttcatc tccggcgtct ggctgatatc cacttttagt ttttcatcca gcggcagtcg
    10741 tgcatccagc acaaccatcg cggtcattaa tttgctgata gacgcaatcg gacgcaccag
    10801 atccgggtgg ttcgagtaga tcactttgtt ggtattcaga tcaacaatca tcgcgctacc
    10861 ggaggcaatt tcaggttgtg aagcggtggt agcggctgcc gttttcgcaa cggcctgcgg
    10921 tgccaaaggc acagccagca tcagggccag gctaaataaa gaaactcgaa atttcggcat
    10981 gatgagcatt cagatagtgg ttcacgcgca caggttgcgc accgccggag taaggattta
    11041 ctgaggctag cgacgccatc ataacgagca aaaagtgcga tcgtcaaagg agaatcgtga
    11101 ggaaatgctg cattgctgac atttacgcca gcaatgcaac gtcaaagaac tttctagaac
    11161 aaacgataac cgtagcccca tagtataacg gttagggcga gcagcacttc cagtaccagc
    11221 acgccaatcg ccagcgtcga actagagaag ctaaggcctt cctctttgtt gatattcagg
    11281 aagctcggaa taccaaggta aagcaggtag ccggtgtaaa acagcgccac cgtgccgacc
    11341 agcgcacaca accagaccag tggataaagc gccaccagac cgcttaaaaa cagcggagtt
    11401 gcaacgtagc ccgcgaagac catgcagtgc gcaagtgacg gacgctgcgg ataattacgc
    11461 gccatccacc agatgacccg ccccattacc gcaaccccag ccagcataac gccataaaac
    11521 aggacagcca gcgccagtcc ggtaaaccag gataacttca ggatagtgcc atcgccaaaa
    11581 ttccagccaa tctgtgtagt gccaatgaag gcgcaaatca ccggaatcgc cgccatcagc
    11641 aaaacgtggt gggtgtaatg gtgagaaatc gtttcgtttt cgcgattaat cacctgcatt
    11701 tcacgatcgg gatgggaaaa cagtccccag acatggctca taccgccccc ttgttgtgag
    11761 ttcatgaacc tgacagttca agtataagtc agcttgtgat tattttttgt tcgctcatgt
    11821 aatttgcacc gtctgatgta cgtccttttt cagcgtgaat gatttccagg gtgtatgctt
    11881 acaggcaacc aaagggagac agactggcct atggatctca atacactcat ctcacaatat
    11941 ggttatgccg cgctggtgat cggtagcctg gcggaaggtg aaaccgtgac tttgctggga
    12001 ggcgttgcgg cacatcaggg gctattaaag ttcccgctgg tggtactttc tgtggcgctt
    12061 ggcggcatga ttggcgacca ggtgctttat ctgtgcgggc ggcggtttgg cggcaagctg
    12121 ttacgccgtt tctcgaaaca tcaggataaa attgagcggg cgcagaaact tatccaacgc
    12181 catccgtatc tgtttgtcat tggtacgcgc tttatgtatg gctttcgggt gattggcccg
    12241 acgctgattg gtgccagcca gctgccgccg aaaatctttc tgccgctgaa tattctcggc
    12301 gcatttgcct gggcgttgat ttttaccact attggttacg ctggtggtca ggtgattgcg
    12361 ccgtggttgc acaatctcga ccagcatttg aagcactggg tctggttgat tctggtcgtg
    12421 gttctggtgg tgggcgtgcg ctggtggctg aaacggcgcg ggaagaaaaa gccggatcat
    12481 caggcgtaaa accattgccc tgggtaaggc gttcgcgccg catccgacat ccggtgcacc
    12541 atgcctgatg cgacgcttgt gcgtcttatc atgcctacca aaattttacc cattgaaata
    12601 accacgcacc tcactgcgct attctgggtt gaactgcgga ttcgccaaca taaagccgcc
    12661 atccactatc aacgactgcc cggtggtgta atttgcgccc tctgagcaca gccacgctac
    12721 caggctggca atctcatgcg ttgcgccaaa acgccgcaga ggaatcgaag gctccgcgtc
    12781 gggcttcacg tcgctgtcat ccatgccatt cattggcgtg gcgatcgccc caggcgcgac
    12841 tgcgttcacc aaaatcttat gcctgaccag ctccagcgcc atcgttttgg ttaacccgcc
    12901 gagcgcatgt ttagcggctg tgtaagcgct ggcatccggc agcggcgtat gttcatgtac
    12961 cgacgtaatg ttgatgatac gaccgccctg cccttgtttc accatctggc gggccgcaat
    13021 ttgtgagcat aaaaatgcac catcgacatc aacggtaaaa atcttgcgcc actcatcgaa
    13081 agccatatca agaaacggcg ctttggtcat tgcgcccgca ttattcacca gcacatcaat
    13141 acgccccagc cgttgaatga gtttctccag cgcctgcgca ccttctggca gtttaccgag
    13201 ctccagctgc acgatctccg cacgtacgcc gtggctaact acctcacgcg cggtatcttt
    13261 tgccccttct tcatctgagt gccaggtaat gccaatatca aacccctgct gcgccagtaa
    13321 taacgcgcac tctttgccga tccccgaatc ggaggcggta ataatcgcaa cctgtgccat
    13381 caagttctcc acttaacgct gaataaacgt taagtataga aggcgcatat catcagcgtt
    13441 tgtaaccccc gcccaacgca ccagtgagtt gaatagaagc atccagccac tgcccttgca
    13501 ataacaggcc attggcccgc tcacgcagcg cggggattct ggcttcgctg acgcgggaac
    13561 caacaatgat gcccgcgtta aagcgcgcct gcgcaagacc taccacacgc aaggcatcgc
    13621 gctcaatttg cgcctgatgc tggtttttct ccgcaagtgt ctgaacctga ctggctgccc
    13681 gcgccacgtc attcaccgct tcaaccaccg ctttgttgta gctggcgata gacaagttgc
    13741 tttcggcttt tgcgatattg agattggcgt taagacgacc actatcgaaa atgggtagcg
    13801 tcaggcctgc cgtaacgccc atttgctgcg cggaatgacg gaacagatcg cttaagtgca
    13861 acgcatcctg ttgcaggaag gccatcaggt tgatgtcagg ataaaatgcc gcttttgccg
    13921 catcaatggt gcttagcgat gactcaacgt accagtgcgc cgcctgcaaa tctgcccgcc
    13981 gggccagtaa ggagtacccc agttcatcag gaagctggct tgccactttc ggcaacgcga
    14041 ccggtttaag cttcaatgac tttgtctggt tatttgtaag tgcgcttaac cgtgcctcaa
    14101 taattttcat tttccccgcg acatcgttga gctgctgccg ggttttgctg gcattaatat
    14161 cggtttccac accttcaact gaagaagtaa tcccgttctg atatagctgg cgatcggtcg
    14221 cgataatggt gttctgctct ttttctattt gctgcaagac cgtgtttaac gccgcctggg
    14281 tttgccactc ccagtacagg cgggctacgc tgccagccag caattggcgg gtttgctcgc
    14341 gttccgccgc ccgtgcttta accgtaccca ggcgggcagt aacctccgcc cgattctttc
    14401 cccagatatc gagatgccag cccgccgtta agccaaaagt accgttggtg taccacgggc
    14461 cggtcgtacc tgcggccgga tcgttcagag caaacggccc cattaagcct tctgccgaca
    14521 ttttttgccg ctccatatcc gccgaaaagt cgatctgcgg accatcctga gtggcaactg
    14581 ccttcgcctg ggcttcagct agctgaatgc gctgttcagc cacctgcata tccggtgcgt
    14641 tctgtagtgc attgttaatt aatgaagtga gctgattatc gtgatactcc agccaccatt
    14701 ggctgtctgg ccaaccattt ttcagcgccg tgggtaatgc ggtgtcaact tgtgcagcgg
    14761 gcgtttgctg gcttaacgcc tggcgggttt catgcatagg cgcacacccg gccagcatca
    14821 gtaacagcgg aaaacaggcg atggctggat aaaaggaatc acgattcatg ggggaataat
    14881 caggtaagaa acggtgcgcg gagattaccg tgtgttgcga tatatttttt agtttcgcgt
    14941 ggcaatacat cagtagcaat aaaacgacat acccagaaaa atatagacta agtaaatgat
    15001 atcttccgat ttatcttaat cgtttatgga taacggcaaa gggcttcgtt ttttcctata
    15061 cttattcagc actcacaaat aaaggaacgc caatgaaaat tatactctgg gctgtattga
    15121 ttattttcct gattgggcta ctggtggtga ctggcgtatt taagatgata ttttaaaatt
    15181 aattaatgtc atcaggtccg aaaataacga gaatatttca gtctctcatc ctgttgcgct
    15241 cctgtcatgt gcattgcttc atataatcac tggcgcaagg agcgcgcagg gggcggccaa
    15301 tcgccgccgc cccctgcacc cccgggctct ggcgaacaaa atcgccgctg cgcggtgccc
    15361 tcggcttatc ccttacggct accgggtcgg gcgcgaggta acatccctgt aaaacgcgcc
    15421 ctcagcccac ctccatgtgg gctgccccgg ccttcaggga acgcctcggc aattttgacg
    15481 ccaccaaaca accgtgcggc ctattgataa agagctaaca cattgtcaaa aaacatcact
    15541 atggtttttt agagtttctc gatatcaatt gcctgaatag cccttgcaat atcaggggaa
    15601 ttattcaaca cccgaacatg ctgaaataat tccgttgctt catcgtattc tttacgcaaa
    15661 taactcaacc actgtttaat ccgcgcaacg tgatataacc cggtatcgcc ctgcttttcc
    15721 agacgggtat atttttgcag caaagcaacc acctccggcc acggcattcg cggttcgtta
    15781 tattttacta cccggctcag gttgggaata ttgagcgccc cgcgaccaat catcactgcg
    15841 tcgcagccgc tgatcgcaat gcattgttgc gcgctctgcc agtcccagat ttcgccgttg
    15901 gcaatcaccg gaatattcag ccgctggcga atctcgccaa tcgcctgcca gtcaatatgc
    15961 tccgcgcggt aaccctgctc ttttgtccgt ccatgcacca ccagctccgt agcaccagcc
    16021 tgttgaaccg catcggcgat ttcaaatttc ttctcaccgc tgtcccagcc cagacgcact
    16081 ttcacgctga cgggcaaatg cgccggtaca gcttcacgca tcgcttttgc accctggtag
    16141 atgagttcag gatctttgag taacgtcgcc ccgccgccgc tgccgttaac cgttttcgac
    16201 gggcagccgc aattgagatc cacgccccag gaacctaatt ccaccgcacg ggcggcgttc
    16261 tctgccagcc attgtgggaa ctgacctaac aactgaacgc gcaccagcgt accggatggt
    16321 gtccggctgg cgttttgtag ctcagggcaa atgcgatgaa agacttttac cggcagcagt
    16381 tgatccacca cgcggacaaa ctcggtgatg cacagatcgt agtcgttaac ttcggtcagc
    16441 aattctcgca ccagagagtc aagcacaccc tccatcggag ccagtaacac acgcatatct
    16501 tcacccggaa aaaaatgagg cgctatgtta gcgcctatgg tcaacggatt aaaggtctgc
    16561 aaagagggat tgctgcaaac tgtaatccct ctctatgctt gatgtatgcc tgagttcgtt
    16621 gcgcattgcg taatgccggg gagcgaaaaa ataagcgctg atttcttaat gtgatcggta
    16681 gcacgtttta cgaattaatt gtatgatgaa tccatctcat ctggggtgtt gattatgagt
    16741 aagacactga acattatctg gcaatattta cgcgctttcg tcctgattta tgcctgcctg
    16801 tatgcaggca ttttcattgc ttccctgcta ccggtaacca ttccgggcag catcatcggg
    16861 atgctgatcc tgtttgtcct gctggccttg caaattcttc cggcaaaatg ggtcaatccg
    16921 gggtgctacg tactgattcg ctatatggcg ctattgtttg tgccgattgg cgtaggcgtc
    16981 atgcaatatt ttgatttgct ccgcgcacag tttggcccgg tagtggtttc ctgtgcagtc
    17041 agtacgctgg tggtttttct ggtggtgagc tggagttcac aactggtaca cggtgaacgt
    17101 aaagtcgtag gtcagaaagg atcagaagaa tgatggcgaa tatctggtgg tcattaccgc
    17161 tgacgttgat tgtctttttt gccgcccgca aactggcggc acggtataag tttcccctgc
    17221 ttaacccgtt gctggtagca atggtggtga tcattccttt tttaatgctg accggcatct
    17281 cttacgacag ctactttaaa ggtagcgaag tgctaaacga tctgctgcaa ccggcggtcg
    17341 tcgcgctggc ctatccttta tatgagcagc tacaccagat ccgcgcgcgc tggaaatcga
    17401 tcatcaccat ctgttttatc ggcagcgtgg ttgcgatggt gacggggact tccgtggcat
    17461 tgttgatggg cgcttcaccg gaaatcgccg cgtcaatcct gccgaaatca gtcaccacgc
    17521 ctattgcaat ggcggttggc ggcagtattg gcggtattcc ggcaatcagc gcagtttgcg
    17581 tgattttcgt cggcatcctc ggcgcggtat ttggccatac attgcttaat gcgatgcgta
    17641 ttcgtaccaa agctgcgcgc ggtctggcaa tggggacagc ctcgcacgcc ctcggtacgg
    17701 cgcgctgcgc cgagctggat tatcaggaag gtgcatttag ttcgctggcg ctggtgttat
    17761 gcgggataat tacttcgctg atcgcaccgt tccttttccc gattattctg gcagtaatgg
    17821 gctaaaattt gcgatgcgtc gcgcattttt gatgtaagtt tcacgcgttg cataattaat
    17881 gagattcaga tcacatataa agccataacg ggttcgtaaa ctgttatccc attacatgat
    17941 tatgaggcaa cgccatgcat ccacgttttc aaaccgcttt tgcccaactt gcggataact
    18001 tgcaatctgc actggaacct attctggcag acaagtactt ccccgctttg ttgaccgggg
    18061 agcaagtctc atcgctgaag agcgcaacgg ggctggacga agacgcgctg gcattcgcac
    18121 tacttccact ggcggcggcc tgtgcgcgta cgccattgtc gaattttaat gttggcgcaa
    18181 ttgcgcgcgg tgtgagcgga acctggtatt tcggtgccaa tatggaattt attggtgcaa
    18241 caatgcagca aaccgttcat gccgaacaaa gcgcgatcag ccacgcctgg ttgagtggtg
    18301 aaaaagcgct tgcagccatc accgttaact atacgccttg tggtcactgc cgtcagttta
    18361 tgaatgaact gaacagcggt ctggatctgc gtattcatct gccgggccgc gaggcacacg
    18421 cgctgcgtga ctatctgccg gatgcctttg ggccgaaaga tctggagatt aaaacgctgc
    18481 tgatggacga acaggatcac ggctatgcgc tgacgggtga tgcgctttct caggcagcga
    18541 ttgcggcggc aaaccgttcg cacatgcctt acagtaagtc gccaagcggt gtcgcgctgg
    18601 aatgtaaaga cggtcgtatt ttcagcggca gctacgctga aaacgccgca ttcaacccga
    18661 ctctgccacc gttgcagggg gcgttaattc tgttgaatct caagggttat gattacccgg
    18721 atatccagcg cgcggttctg gcagaaaaag ccgatgcgcc gttgattcag tgggatgcca
    18781 cctccgcaac gctgaaagct ctcggctgtc acagtatcga ccgcgtgctt ctcgcttaag
    18841 cctggtgccg gatgcggcgt gaacgccttg tccggcttgc cagccctctc ttggtgtcga
    18901 aattcccggc aaacagtttg ccgtttcttg cgcaaaacca gcgggtaaag tagcctgatg
    18961 gaaattttcc ttagatcgag tctcctgcat gttaaagcgc gtgttcctca gcctgttagt
    19021 cctgatcggc ttgctgctgt tgactgtgct cggcctcgat cgctggatga gctggaaaac
    19081 cgcgccttat atctacgacg aattgcagga tctcccctac cgccaggtcg gcgtggtgct
    19141 cggcacagca aaatattatc gtactggcgt aattaatcag tattatcgct accgcattca
    19201 aggagcgatt aatgcctata acagcggtaa ggtaaattat ctattactga gcggcgataa
    19261 cgcattgcaa agttataatg agccgatgac catgcgcaaa gatttaatcg ctgctggtgt
    19321 cgacccatca gatattgttc tcgattacgc aggctttcgt acgctggatt ccatcgtgcg
    19381 tacacgcaaa gtgttcgata ctaatgattt cattattatc acccaacgtt tccactgtga
    19441 gcgggcatta tttattgcgc tgcatatggg gattcaggct cagtgttatg ccgtaccgtc
    19501 accgaaagat atgctgtcag tacgtattcg cgaatttgcc gcccgtttcg gtgcgctggc
    19561 tgacctttat atttttagac gtgaaccgcg ttttttaggg ccgctggtcc ctattccggc
    19621 tatgcaccag gtgccggaag atgcgcaggg gtatcccgcc gtcacacccg aacagttact
    19681 tgaattacaa aagaaacaag gaaagtagtt atggatgtac agcagttttt tgtcgttgcc
    19741 gtttttttcc ttatcccgat attttgtttc cgcgaagcat ggaaaggctg gcgcgcaggc
    19801 gcgattgata aacgggttaa aaatgcaccg gaaccggtgt atgtctggcg agcaaaaaat
    19861 cccggactct ttttcgctta tatggtggca tatatcggct tcggaatttt atctatcggc
    19921 atgattgttt atcttatttt ctatcgttaa tattttctct tttctgttaa ttattgagaa
    19981 taattattac ttcactcaat aagctgcgga tataacattc tgaaccgcag ctatttgtga
    20041 atcctttcac agtttaaatt tccccacacg cttagcctta atatcagcac gcaatattta
    20101 tttagcgttc gccttaatta ctcatagcat taaggaagat cacatgccgc aacaaaatta
    20161 tctggatgaa ctcactccgg cttttacgcc tttactggcg attaaagaag cctctcgctg
    20221 tttattatgt catgacgctc cctgtagtca ggcttgcccg gcacagaccg atccggggaa
    20281 atttattcgc tcaatctact ttcgtaattt taaaggcgct gccgagacaa ttcgcgaaaa
    20341 taatgccctc ggtgccgttt gtgccagagt gtgcccgacg gagaaattat gccaaagcgg
    20401 ttgtacccgt gccggtgttg atgctcccat tgatatcggc cgcttacagc gttttgttac
    20461 tgattttgaa caacaaaccg ggatggaaat ttatcagccc ggtactaaaa cgctcggcaa
    20521 agtcgcgatt attggcgcag gtcctgccgg attgcaggcc agtgtgacac tgacaaacca
    20581 gggttatgac gtcacgatct atgagaaaga agcacaaccc ggtggctgat tgcgtaacgg
    20641 cattccgcaa ttccggttac cgcaatcagt actggatgca gagatcgccc gtattgaaaa
    20701 aatgggcgtg accattaagt gcaacaacga agtcggtaac acactcaccc ttgagcagct
    20761 gaaagcagaa aaccgcgcgg tactggtcac cgtggggtta tcaagcggtt ccgggctacc
    20821 gctgtttgag catagtgacg ttgagattgc cgtcgacttc ttgcaacgtg cacgacaggc
    20881 gcaaggcgat atcagcattc cacaaagcgc attaattatc ggcggcggtg atgtcgcgat
    20941 ggacgtagcc agcacgctga aagttctcgg ctgtcaggcg gtaacttgcg tagcgcgtga
    21001 agagttagat gagtttccgg caagcgaaaa agagtttacc agcgcccggg agctgggcgt
    21061 ttcgatcatc gatggattca cgccagtagc cgtggaaggc aataaagtca cgtttaagca
    21121 tgtacggtta tcgggcgaac tgacgatggc ggcagataaa attattctcg ccgtcggtca
    21181 gcatgccaga ctagatgcct ttgcggagtt agagccgcag cgtaacacca tcaaaacaca
    21241 aaattaccag acccgcgacc cgcaagtctt tgctgctggc gatattgttg agggagacaa
    21301 aaccgtggtc tatgccgtga aaaccgggaa agaagccgcc gaggcgattc atcactattt
    21361 agagggagct tgctcatgtt aacgaaagat ctttcgatta ctttttgcgg cgtgaagttt
    21421 cccaacccgt tctgcctctc ttcttcgccg gtaggcaact gctatgagat gtgtgccaaa
    21481 gcctacgaca caggttgggg cggtgtggtg tttaaaacga tcggcttttt tatcgccaac
    21541 gaagtctcgc cgcgttttga tcatctggtg aaagaagata ccggttttat cggcttcaaa
    21601 aatatggagc agattgccga gcatccgctg gaggagaatc tggccgccct gcgtcggctg
    21661 aaagaagatt acccggacaa agtattgatc gcttcgatca tgggggaaaa tgagcagcaa
    21721 tgggaggagc tggcgcgcct ggtgcaagaa gctggcgcgg atatgatcga gtgtaacttc
    21781 tcctgtccgc aaatgacttc tcatgcgatg ggtagcgatg tcgggcaaag cccggagctg
    21841 gtagaaaaat attgtcgggc agtgaaacgg ggttccacgc tgccaatgct ggcgaagatg
    21901 acgccgaata tcggtgatat gtgcgaagtg gcgctggcgg cgaagcgcgg cggcgcagat
    21961 ggcattgcgg cgattaacac cgttaaatcc atcaccaata tcgatcttaa tcagaaaatc
    22021 ggtatgccga tcgttaacgg aaaatcaagt atttccggat attccggtaa agcggtaaaa
    22081 ccgatcgccc tgcgcttcat tcagcaaatg cgcacccatc cagaactgcg cgatttccca
    22141 atcagcggta tcggcggcat tgaaacctgg gaggatgcgg cggagttttt attgctcggc
    22201 gcagcaacgt tacaggtcac caccggcatt atgcagtacg gctatcgcat cgtagaagat
    22261 atggcgagcg ggttatcgca ttatctcgcc gatcagggat ttgattcgtt gcaggagatg
    22321 gtaggtctgg cgaataacaa tattgtcccg gcggaagatt tagaccgcag ttatattgtc
    22381 tatccccgta tcaatcttga caaatgtgtt ggctgtggac gctgttatat ttcctgttac
    22441 gacggcggtc accaggcgat ggaatggagc gagaaaaccc gcacaccgca ttgtaatacc
    22501 gagaaatgtg tgggttgtct gctttgtggt cacgtctgcc cggtgggttg tattgagctc
    22561 ggggaagtga agtttaagaa aggcgagaaa gaacacccgg taacgttgta gaacccgcgt
    22621 cacatccggc atttggttcc tgttgccgga tgcggcgtga acgccttatc cggcctacaa
    22681 aaccacacag atcatgatgt tatgtgcgtt tatgaccgaa tgcggaccac attcacatca
    22741 tttcttacgc gcgtatttca gtgaatccag cgctacggcg aagataataa tcgccccttt
    22801 gatgatgtac tgccagtatg ggtttacgcc gatatacgtc aggccatagt tgatgacggt
    22861 aaaaataatc accccggtca ccacgccaat caccgtcccc acaccgccgc tgaacgatac
    22921 accgcctacc acgcacgccg cgatagcatc cagctcatac ataaagccga ggttgttggt
    22981 ggcagagccg atacgtccgg cttctaacat cccgccaaag gcatagaaca cgccagacaa
    23041 cgcgtagatc atcagcaggt tcaggccgac gttgacacca gatacttttg ccgcttccgg
    23101 gttaccgcca atggcaaaaa tgttcttacc gaagcgggtt ttgttccaca acacccagac
    23161 gaacgccacc gcaatcaacg cgtagaaggt gatgtaagag agacggaaac tccccagcgc
    23221 gacaaagccc tgagcaaagg tagagaagcc actgtcaaaa ccagaaattg gcgacgcccc
    23281 gacaaagtcg taatagagcg agttgatgcc atagacgatg atcatcgtgc cgagcgtggt
    23341 aatgaacggc gtcacgttga gataagcgat aatcagaccg ttgatcaaac cgatcaccgc
    23401 accaatggca cagacaatca gaataaccag cgcaatcggc atcgtcgcca tttccgggaa
    23461 cactttgttg gcgttatcca tggactgcaa taatgtcgca gccaccactg ccgccagccc
    23521 tacctgacga ccagcagaaa gatcggtccc ctgggtgaca attaaccctg ccacaccgag
    23581 cgcgataata atacgcaccg atgactgggt gagaatatta cttaagttca acagacttaa
    23641 aaatgttggg tcctggaaaa taataatcgc cagcaaaact aaaagaacga cgtaaatacc
    23701 gccctctttc aggtaagtaa gaaaactttt cttatttaac gcactcatgg gaagccccta
    23761 atcttaaagg tgcaaagacg caagacgcag aatttcgttt tgcgttgttg tttttgtatc
    23821 gacaattccg gaaacgagac cattgctcat gaccagaata cggtctgtta tccctaacaa
    23881 ctcaggcatt tcagaggaga taataataat ccccttgcct ttcttcgcca gttcggcaat
    23941 taactgataa atttcaaact tcgccccgac atcaataccg cgcgtcggtt catcgagcat
    24001 taatatttct ggttgcgtta gtagccagcg accaataatc accttttgct gattaccacc
    24061 ggagagcgaa ccaatttgcg tccgatgacc cggcgttttt acccgcatcg aatcaatcac
    24121 ccactgggta tcgcttttca tccgcgagtt atccagtaaa ccaactttat ttttgtagtt
    24181 gcgaatattg gaaattaacg agttaaaacc aatatccaga taggcataaa ttcccgttga
    24241 gcggcgctcc tcagttacca gtgcaaatcc gtggtttatg gcttcgttgg cattatggtt
    24301 attgatctgt ttgccgtgca acgtaatggt gccagccgat ttctcgcgaa taccaaataa
    24361 cgtctcaaca atatcggtac gtttcgcccc caccagcccg gcaataccga ggatctcccc
    24421 tttatgcaga tcaaacgaga catcgcgaat cgacggctgg cgcagtgacg ttaggttacg
    24481 cacctcgagg atgacttcgc ccggcttgtt ttctttgtca ggaaaacgct gattaagaga
    24541 acgcccaacc atcatggcga tgatcttgtc catcgtcagt cctgccagcg gttcggtggc
    24601 gatccactga ccgtcgcgca atacggtaac ttcatcacat aactggaaga tttcttccat
    24661 tttatgcgag atataaacaa taccgcagcc gcgctctttt aatttacgaa taatagtgaa
    24721 cagatgattg acctcttttt cggttaacga agaagtcggt tcatccataa tcacaatttt
    24781 cgcgttatag gaaaacgctt tggcgatttc gatcatctgc atttgcgaaa cggataatgt
    24841 gccgacgcgc gcacgcggat cgatatcaat atccagttca tcaaaaattg ctttggtttc
    24901 gcggtacatt ttgtcctgat cgacaaacat gcctttggtg ggatatcgcc ccagccacat
    24961 gttatccatc accgaacgtt gtaataccag gtttaactcc tggtgtacca tcgaaatacc
    25021 attttccagt gcctctttgg cggaatggaa atcgatctct ttaccctgga ataaaatggt
    25081 gccggagtct ttttgataaa taccaaacag gcattttaat aatgtcgatt ttcctgcacc
    25141 gttttccccc attaatgcat ggatagaatg tggccggact tttaaattaa cgttatcaag
    25201 tgccttaaca ccaggaaagg acttgttgat accgctcatt tccaacaagt attccccgga
    25261 agacggagtc gttgagctga ccatataatt ttaccttgtt ggccatacaa taagggcgca
    25321 gtaaacgact gcgcccaatc agtcttattt cttgctgaat tcagccaggt tgtctttatc
    25381 tacgccaaca taaggtacgc ggaccacttt gttgtcgatt ttccagttgg tgccatcagc
    25441 cgcacctttg ccatcggcca ggtttttcgc cagatcaaag gtcgctttcg cctggttgtt
    25501 agcatcgttc agtactgtgc ccgccagtgc accggatttc accagcgcca gcgcttctgg
    25561 cagcgcatcg acgccaaaca ccggaatgct ggacttgttg tgtgctttca gtgcttctac
    25621 cgcgcccatt gccatcgcat cgttgttggc gataaccact tcgattttgt tggcgttcgg
    25681 gccagacagc caggcgtcca tcttatcttt cgcctgagcg gtgtcccaca ttgcggtatc
    25741 taactgtaac tgttcagttt tgatgccttt atcgttcaat tctttaatca cgtaagtggt
    25801 acgtgcttct gcatccggat ggcccggttc acctttcagc agtacgaact gaatctgacc
    25861 gtctttgttc agatcccaac cctgattcgc cgcccagtgt ttagcaatca aatcgccctg
    25921 aataatgccg gactctttgg agtcagtgcc aacgtagtag gctttgtcgt agctatccag
    25981 cgccttacga gacggttctt tgttgaagaa aaccaccggc acgttttgcc cacgcgcttt
    26041 ctcaatcacc gtacccgcag ctgccgggtc aaccaggttg attgccagtg ccttcacccc
    26101 tttcgccagc aatacgtcga tctgatcgtt ctgcttggac tggtcattct gagaatcatt
    26161 catcagcagc tgaacatctg gcgcggcttt cgcatcttgc tcaatagcct tgcgcactac
    26221 agacataaag ttatcgtcgt acttatagat tgttacacca atgcgagtat cagcagcgtg
    26281 tgcagcggca ccgaataaca tgctggccat cacagcagac agggttaaca ccttcttatt
    26341 catggtatct ccggtttttc ttatgcaggg tagtgcttga gataaatgct cggcggggca
    26401 gtagagttaa tgaagtgtta ctgaacgccg aagctcactt ttttaaattc gttcttccat
    26461 gcccggtaac gctccagaaa acggctttaa ttgttgttat gacgctatta cctcggtgaa
    26521 agtgattatt cactgttaca tacgggttac aacgttaaaa cggtgcaatc atagctatca
    26581 cattgttaag atactgtgaa atcactcaca gattgaaagc ggttacatcg cctgatttgt
    26641 tgagttagtg atcgccgccg cattctggcg cgttgcgaca gaatggcgac gcactaacgt
    26701 cggcataaaa cagtggctgg cacgaggatc aatattgcct gctgccccct gcaaggccag
    26761 ttcggtggct aatttagcca ttgaagcaat gggataacgc acggtcgtta attgcgggtc
    26821 ggtgtaacgg gcgatgggaa tatcatcgaa accgatgatt gagagatgta acggaatcgc
    26881 aatgccatta tcttttaatg ctgtcagtgc gccagcggcc atattgtcgt tataagcaaa
    26941 tatagcggta agttgtagat tgcgccccag cagttcaacc atcgccgcct caccgcccgg
    27001 catgtccggc gtaccagtgc caatccagct ttccggcgga ataatatcct gctctttcaa
    27061 cgcactcatc cagcctgctt tacgcatggc gtcatcttca atgccgtgac tggaagaaag
    27121 ataaccaata cgttgatgac cgttattcag caacatgcgc gtcgccattc gggcaccgct
    27181 gagattatcc aggcaaacgc aacgatgggc gtaccccggc acaacgcggt tgattaacac
    27241 cataccggga atgttatcca taaattgcgc cagttcatcg tcactcaatg cttttgagtg
    27301 aacaatcaac gcattacaac gctggcgaat taacacctca atggcgtgac gctctttttc
    27361 cgcttcatga tagctattgc cgattagcac gtatttctga tgctgctgag cgactagatc
    27421 caccgctttt accagcgcgc cgaaaaacgc atcagaaacg tccatcacca ccacgccaat
    27481 ggtgtcgcta acctgagttg ccagcgcctg ggcattggcg tttggccgat aatccagctc
    27541 actcacggct ttcattactg cttcacgcgt gtcggcactg accagcgtgc tgttattgag
    27601 cacccgggaa accgttgcca cagagacgcc agcctaacgc gctacatcac gaatggtgat
    27661 catattcact acctgttatg agattgcagt aaatgactgc ttgctggcgg ctattttgtc
    27721 agcactgaat acaggacttc gtgaatcgag tcacagcaat ggaaacggtt acagccgttt
    27781 tgttaacgaa tgtgatccag atcgttatct tgatgtttta gatattgccg ttccggcagc
    27841 acgcaaagtt aactgacgcc agagccattc caccggcccc tggcggaagt aacgcagcca
    27901 gataacagag aagaggatat tcgccagcca taccggaata acaaacgcca gcagctccag
    27961 gcggtcaaaa tgcataaaca aaccgaggtg gtaaaaaagc atggtacaaa tcagcgtttg
    28021 caatagatag ttggtcagcg ccatccgtcc gacgcaggcg atcgcaagca ccagcttaaa
    28081 gcggctcaat tgcggccaga agccataaaa cagcgacgca tagccaatcg cctgaaacgg
    28141 cgcactcagt tcccgcggca tttgaagtaa gaaggcacac cagcgatacg cccagtccag
    28201 ctgccattgc agggcgatgg caggaaggtt aatggtcacc ccaatcgcca ccagcacaaa
    28261 accagtacga cgatagtgac gtaagctgaa ctgccctttc agccagccgc tgcgcatcag
    28321 cgccgcacca atgagcatca tccctgccag ttgccagcca tactgtgcgc ccagtgccag
    28381 taaactgttg ccaacaccat cggcacgatt actgatcgct tcaacgccgc cgtgaagctt
    28441 ccagtatttt tcatacaaaa tagccgatgc atccggcgtc caggtgcggc tggtctggct
    28501 gtcggaaatc agccccaata acagcaaaac gccaagcccc accagataaa gcatgacgcc
    28561 tgtattaaac aggcttttta ccgatggcgc atcgcgcacc agccgccagc agattaagcc
    28621 caccagcccg taagccagca gaatatcgcc gtcccagaac aataaaccgt gaataaaacc
    28681 cagcaagact aacagcgtta accgcgactg gatccagcgt ctgccacggg gcagcaacat
    28741 ttgcaggccc gcaccaaaca gcagcgcaaa aagcgtgagg aatttcacct ggccgatgag
    28801 atcgagaaat gcccaggtcc atgcatcctg cggcgtaata gcgccgtacc aggcgggatt
    28861 gagataagcc gcctttggta gcccaaaggc gctgatgttt aatagcagga tccccagaat
    28921 ggcgacgccg cgaacaaaat cgagcgttac gttgcgctcc atggttcctg ccttttaatc
    28981 agttgtgatg acgcacagcg cgcagaaact cgtggcgcgt attctgactg gatttgaaca
    29041 atccaccaag agaggtcgtt gttgtggcac tggttgcatc gcggatgcca cgcgctttca
    29101 cgcagtaatg caccgcatca atggagacag ccacgttatt ggtgcccagc agcgtttgta
    29161 gcgcaataag aatttgctgc gtcagacgtt cctgcacctg cggacgctgg gcaaagaact
    29221 gcacgatacg gttaattttt gacagaccga tcaccgaatc tttcgggata taagccaccg
    29281 tcgctttgcc atcgatggta acaaaatggt gttcacaggt actggtcaga gtgatatcgc
    29341 gcacggtgac catttcatcg accttcattt tgttttcaat gagggtgatt ttcgggaagt
    29401 tggcgtaatc cagaccggag aaaatttcat cgacatacat tttagcgatg cgatgcggcg
    29461 tttccatcaa actgtcatca gccaggtcga gattcagcag ctgcatgatt tcggtcatat
    29521 gaccagcaat aaggcttttg cgcgtttcgt tatccatttc atgcacgggc gggcgcagcg
    29581 gtgtttccag tcctcgcgca actaacgctt catgaaccag ggccgcttct ttactgagtg
    29641 atggcattta tgatttctcc tgcaggtgtg acgcctccgc cctgcgtggg ggcaaagtta
    29701 ttaagctgat ttacagcctg attattgtgc gtgaggcggc gcacataatc cagtattcac
    29761 agcgataatt attgtaattg ccgctgactt tcatcagcag atgttaaaac atcgttatgc
    29821 aaatacggaa gtgaaagtta ctcacagcac attgaataaa cggtatgatg aagaaattgc
    29881 aaacaacaca acaaggagcc acgcatggaa atgctcgaag agcaccgctg ttttgaaggc
    29941 tggcagcaac gctggcgaca cgactccagt accttaaact gcccgatgac gttcagtatc
    30001 tttctcccac cccctcgtga tcacaccccg ccaccagtgc tgtactggct ttccggatta
    30061 acctgcaatg acgagaactt caccaccaag gcgggtgccc agcgcgtggc ggcagaactg
    30121 gggattgtac tggtgatgcc agacaccagc ccgcgcggcg aaaaggttgc caacgacgat
    30181 ggctacgatt taggccaggg cgcaggcttt tatcttaatg ccacgcaacc gccgtgggcg
    30241 acgcattacc ggatgtatga ttatctgcgc gatgaattac cggcgctggt tcagtcgcaa
    30301 tttaatgtca gcgaccgctg cgccattagc ggtcactcaa tgggtggtca cggtgcgctg
    30361 attatggcgc tgaaaaatcc gggtaaatac accagcgttt cggcctttgc gccaattgtg
    30421 aatccgtgca gcgtcccgtg gggaatcaaa gcgtttagca cctatttagg tgaggacaaa
    30481 aatgcatggc tggaatggga tagttgcgcg ctgatgtatg ccagtaacgc gcaggatgcg
    30541 atcccgacgc ttatcgatca gggcgataat gatcagtttc ttgccgacca gttgcaacct
    30601 gcggtattgg cagaagccgc gcgccagaaa gcgtggccga tgacgctgcg tattcagccg
    30661 ggatatgatc acagttacta cttcatcgcc tcttttatag aggatcacct gcgcttccat
    30721 gcgcagtatt tactgaagtg aaagtccgcc cggttcgccg ggcatcttct catcagaagc
    30781 gataatccac tgccataaag taacgacgtc cgtcttcgtt atagctgtag tcgtcacgac
    30841 tgagatcctt gtcgccaagg ttcagcacgc ctgcgcgcag tttgacgtct ttagtcacct
    30901 gccaggccgc gccggtattc cagatggtat aaccgcccgg tgttttagcc gtcgcgctgt
    30961 cggcgcgttt ctgcccggta tagtgaccag aaacatagaa tgaccagtct tccagcgcca
    31021 gcggtttcca gtccagcgta ccgttagcag tatggaacgg cagatcggat agcggtttgt
    31081 tttcgccgtt gctgacatca cgaccatcgt tgtaggtgta gttgatcgac agtttccatt
    31141 catcgttgaa cggaattttc agttcggttt ccacgccctg aatacgagct ttgttaacgt
    31201 tgtagtagct aaataccggt atgcgccgtc cgttagcgcc cgtctcaaaa ccaacaaagt
    31261 tttggtagcc cggtgcagcg ttgacgtcag acgtacggct gatgctgata cgatctttca
    31321 catcgttacg gaaaacggta acgctggatt caaccccttc cagccagcct tcttcaccca
    31381 tgtagtaaag ccccagctcc caactttcgc tggtttctgg tttcagatcc gggctaccca
    31441 caatcttaca tgcgccacgg caggaattgc tcgtccagtc agggctaagt tgcaacagag
    31501 aaggtgcttt aaatgccgtc gcccagcccc ctttcaccgt tacggtgtcg gtggcgttat
    31561 aaaccaggta ggcacgcgga ctccagtgtt caccgtaggt ttcgtgatcg tccatacgca
    31621 cgccggtcgt cagcgccagc ggctcgaaga tccgccattc atcttccaca aacagcgcgt
    31681 actggctggc agacgttttg gagctggttc ccccggtcag gttcaccgca tcgctaagtt
    31741 tgtcgtgacg ccattcaccg ccaaccgtga gaaactgatt aatcgccgtc agcggcaacg
    31801 tgtatttgcc gtcgaccgta ttgctttcgg aagttatcgg gctgctgttg ccagggtttt
    31861 tgttctcgac tttctcaccg tagtatttca gttcgctggt gccgtaatcc caacgcccat
    31921 tatggctgac ggagtagttc tggcgttcca ggcggttttt gtccagcgag tcggaatcac
    31981 gatcctgacg gtcgaaaccg tatccggcag taaaatcgtg attttgattc ggtgtccagg
    32041 caaattcgac attgccgtcg cggctggaga atccttcaat acgcggcgtt tctccggtat
    32101 cggtggtcgt tgagttttgc gggtcatcct tttcacgttt tgccaggctg ccgtaagctt
    32161 tcattcccag cacaccatca attaatggtc cactggtaaa gaactgaccg ttataggtat
    32221 caccgcgatc gcgatgttcc tgaatggtgg tatcgacggt aacggtaccc gaccatttct
    32281 gaccgatttt tttggtgatg atattcacta caccgccgag cgcatcggaa ccgtacagcg
    32341 acgacatcgg gccacggacc acttcaatac gttcgatgga atcgaccggg atccagttca
    32401 gatcgaaatc attgtggcgg aagacggcat tgcgggagtt cacgcgttta ccgtcgacga
    32461 gaatcagggt atagctgctg tccagaccac gaatactaac gcccttacgg ttatcccctt
    32521 cgttcgtcag ttgtacgcca ggcacttctt tgaggacatc cttcagattc tgtaccggtt
    32581 ttcgctgcag atcttcctgg gtaatgacgc tgatactggc gggtgcatct ttaaggtttt
    32641 gttcaacgga agatgcagtg acaaccatcg tttcgccatc gtcatcgacc gctaacacag
    32701 gccatacaca agaaatagcg gacaaacaca gcccgacccg tacgaaaggg ttcaacctaa
    32761 acattccata tctccatgag gtaactacga aaataaaatg ggtatcgctc acatcttctt
    32821 tacgtcccct tgcgtacggc agcatcgcgg tggacttatt tgtatgcagg tgatcatccc
    32881 agaaagccgg accagcctct gatttgtcag ccttttttga ttgtagcgta acgataatgc
    32941 aaacgataac aattatcaat ccaaatgtta aattttatat ccgcatgatt tgtcagggaa
    33001 atggaattga aaaagccctc tcggttgaga gggcttagca aggaagggag gaaacttatt
    33061 tcttatcgtt ctgcgggaac ttcatttcgc tgtagcgtac gaagtgagtt cctttaatca
    33121 gcttgtagcc gaaccaaata atcaggaaca gcgggatacc aatatacgtt gccgctacgc
    33181 cgccccagtc aatggtatct ttcaggaacg cttcgtagtt ctggcccaaa gtgataatca
    33241 gacacagaat gaatgcgaag atcggcccca gtgggaagaa acctgaacgg tacggcagat
    33301 cgttaatgtc gtgtccctgc aatacgtaac cgcgacggaa gcgatagtgg ctaatggcaa
    33361 tccccagcca ggcgataaaa cccgtcatcc cggaggtgtt cagcagccac aggtataccg
    33421 tctggttgcc aaacatggag gtcaggaagc acaaaccggc aatcaccgtc gtcgcataca
    33481 gcgcattacg cggcacgcca ccacgcgaca atttagcgaa aatgcgcggc gctttaccgt
    33541 cacacgccag ggtgtacagc atacgagtag acgcatacat accggagtta cctgctgaca
    33601 gcaccgccgt cagaataact gcgttcatca ccgccgccgc agagagcaga cccgcgtgct
    33661 ggaacaccaa ggtgaacgga ctgacgctga tgtctttaac atcgttacgc agcaggctcg
    33721 gatcggtata cggaataatc aggctgataa tcaggatcgc gaacacatag aacaacagga
    33781 ttcgccagaa tacctgacgt accgcgcgtg gaatgttttt cgccggatct tcggactcgc
    33841 ctgcagcaat accgatcagc tcggttccct ggaaagagaa gccgacaatc atagctacgc
    33901 cgatcatcgc cgcaaaacca ccagcaaacg gcgcttcgcc gattgtccag ttgctccagc
    33961 ccgcaggctg cgcgcctttg aagataccga taatcatcag cacgccaacg atgataaaga
    34021 caataactgt cgtgactttg atcagtgaaa accagtattc cgcttcacca aagccacgaa
    34081 ctgagatgta gttcagcagg aagataacgc cgaggaacaa cgcactccag atccagcccg
    34141 gtgtatccgg gaaccaccag ctcatgacca gctgtgctgc aaccaagtca acggcaatag
    34201 tcaccgccca gttgtaccag tagttccagc ccagcgcgaa gccaaagcct tcttcaacat
    34261 agttctgacc gtaagtggca aacgaaccgg aaaccggcat atatgcagcc aattcaccga
    34321 gactggtcat caggaagtaa accatcaggc caatcagcat atacgagagc aatgccccgc
    34381 ccggacctgc ctgagaaatc gttgcgccag aggcaacaaa aagacctgta ccgatggaac
    34441 cgccaatggc aatcatcgtc aggtgacgcg cctttaattc acggcgtaag cccggcgctt
    34501 ctgtagtttt agtttcggaa cccatacaaa aatgctatcc atcttaaaaa tgaggcgcga
    34561 ttgtagcaga cgattggcaa tccttccggc agaaatacac ggttataaga caccttcatg
    34621 atcgcccagg gattataagt aaagcagcca atctcttttc tggcgaatgc atgctgaggg
    34681 cgctgcgtca cgaaatcgac acagcaccag catgttcttg tacagcacct taacgcggta
    34741 cattcgcggg atcgcaatag tccagaaagc gccgtagcgc gttggaaagg tgtttttgcc
    34801 gatgatgtat acgccacaac gtacgcatca ggcgcgggag agggaccgca acttcactta
    34861 atgtgcctgc ctgcaattga tcttcaatca cacgtcgcga caggcaacta attcccaacc
    34921 catgacgcac cgcatgtttg attgcctcgg agttacctaa ttccatcgcc atctcaaact
    34981 tcggtaaatg tgacagcaac agataatcga caatctcccg cgtgccggaa ccgcgttcac
    35041 gcaggatcca cggcgcagcg gccagctgtt ctaaggtgac cggaccacga gccaacggcg
    35101 aagtcggcgc ggcgaaaacc accagctcgt cttccagcca cggttcagaa atgatttcag
    35161 tgctgtggca cggtccttca ataaagccaa tatcaacgcg gaaatccagc accgcttgca
    35221 tcacgtcctg gctgttccca acgctaagtt caatcggcaa ctgcggataa tcatggcgat
    35281 aactgcaggc agaatgtagt taccgatggt actactggca tagatacgaa tcgcgccgtt
    35341 gtcttcgcga aacagttgtt cgatttcaac cgcctgttca agcaatgcca acgcacgcgg
    35401 atagagcagc cgcccgtgtt cattaacaac cagtcttttc cccacgcgat caaacagttg
    35461 cacgccaagc tgcccttcca ggtcggtcaa ggctgcgctc actgctgatt gcgacaacgc
    35521 cagcatcacc gacgcctggg tggttgatcc acttttcaat acttctgcaa aaacttccaa
    35581 ctgccggagg gtgatgtgca tagtcgctta ccacttataa agattaatta taaatatata
    35641 atcaatttta tttttaaacc agttagtcgt aaccttatgc ccggttaaag gagagggtta
    35701 tgacgaatat caccttacag aaacaacatc gtacactgtg gcattttatt ccggggttag
    35761 ccctgagtgc agttatcacc ggggtcgccc tgtggggtgg ttccattccc gcggttgcgg
    35821 gtgccgggtt tagtgccctc accctcgcaa tcttgttggg gatggtttta ggcaacacca
    35881 tctatccgca catctggaaa agctgtgacg gtggtgtgct gtttgccaag caatatttat
    35941 tacgtctggg tatcattctt tatggcttcc gtctgacgtt ctcgcaaatt gccgatgtcg
    36001 gtatcagtgg gatcatcatt gacgtgttga cgctgtccag taccttcctg ctggcttgct
    36061 tcctggggca gaaagtgttt ggtctggata agcacaccag ctggttgatc ggtgccggta
    36121 gcagtatctg tggtgctgcc gcggtactgg cgactgagtc ggtagtgaaa gcggaagcca
    36181 gtaaagtaac cgtggctgtt gcaaccgttg ttatcttcgg gaccgtcgcg attttcctct
    36241 acccggcgat atatccgctg atgtctcaat ggtttagtcc ggaaactttc ggtatctaca
    36301 ttggttctac tgtgcacgaa gtggcgcagg tggtggcggc aggtcatgcc atcagcccgg
    36361 atgcggaaaa cgcagcagtt atttccaaaa tgctgcgcgt gatgatgctg gctccgttcc
    36421 tcatcctgct ggcggcgcgt gttaaacagc tgtctggggc gaacagcggc gagaaaagca
    36481 aaatcactat tccgtggttt gccatcttgt tcatcgtcgt tgccatcttt aactcgttcc
    36541 acctgttacc gcagagcgtg gtgaacatgc tggtaacgct ggataccttc ctgctggcaa
    36601 tggcgatggc ggcgctgggt ctgaccactc acgtcagcgc gctgaaaaaa gctggggcga
    36661 aacctctgct gatggcactg gtactgtttg cctggctgat tgttggtggt ggtgcgatta
    36721 actatgtgat tcaaagcgtc atcgcataaa ccactacatc ttgctcctgt taacccgcta
    36781 tcattaccgt tttcctccag cgggtttaac aggagtcctc gcatgaaata cattggagcg
    36841 cacgttagtg ctgctggcgg tctggcaaat gccgcaattc gcgccgtcga aatcgacgca
    36901 accgcgtttg ccttgttcac caaaaaccaa cgtcagtggc gtgccgcacc gctcacgacg
    36961 caaaccatcg atgaattcaa agccgcctgt gaaaaatatc actacacatc ggcgcaaatt
    37021 cttccccacg acagttatct gattaacctc ggacatccgg tcactgaagc tctggaaaaa
    37081 tcgcgcgatg cctttataga tgaaatgcag cgttgcgaac aactggggct ttctttgctc
    37141 aacttccacc ctggcagcca tctgatgcag atttcagaag aggattgcct tgcgcgtatt
    37201 gccgaatcca tcaacattgc gctggataaa actcaaggtg taacagcggt gattgaaaac
    37261 accgccggtc agggcagtaa cttagggttt aaattcgaac atctcgcggc gattatcgac
    37321 ggcgtggaag ataaattccg cgtcggcgtc tgcattgata cctgccatgc tttcgctgcc
    37381 gggtatgatt tgcgtactcc agccgaatgc gaaaaaacgt tcgctgattt tgcccgtatt
    37441 gtcggcttta agtatctgcg cgggatgcac cttaacgatg cgaaaagtac ctttggcagc
    37501 cgcgttgacc gccatcatag cctcggtgaa ggcaatatcg gtcatgatgc gttccgctgg
    37561 atcatgcaag acgaccgttt cgacggcatt ccgctgatcc ttgaaaccat caacccggat
    37621 atctgggcag aagagatcgc ctggctgaaa gcgcaacaaa ctgaaaaagc ggtggcctga
    37681 tcgatgaacg acagggaaaa gcagattctt aaaattttac ggcgtaaccc gctgattcaa
    37741 cagaatgaaa tcgccgatat tctgcaaatc agccgctcac gtgttgccgc tcatattatg
    37801 gatttgatgc gcaagggttt gattaaaggc aaaggatata tccttaccga gcaggattac
    37861 tgtgtggttg tgggtgcaat caatatggat attcgcggaa tggcggatat ccgttacccg
    37921 caagcagcct ctcatccagg ttctgttcac tgttccgcag gcggcgtcgg acgtaatatc
    37981 gcgcataacc tggctctgct tggtcgggat gtgcatttaa tatcagctat tggcaacgat
    38041 ttttacggcg agacattact ggaagaaacg cgccgcgcgg gcgtgaacgt ttcaaactgc
    38101 attcgtctgc atggacacag cactgcaact tatctcgcta tcgctaataa gcaggaagaa
    38161 actatcctgg caatcaatga cacacatatt ctgcaacagc taacgcctca actgctcaat
    38221 acttcacggg acttgattcg ccatgctggt gtggtgctgg ctgattgtaa tctcacacct
    38281 gaagccctgg agtgggtctt taccattgct gatgaaattc cgatgtttgt cgataccgtt
    38341 tccgaattta aagcgaacaa agtaaaaaac tggtacagcc ggatccacac tctgaaacca
    38401 acacaaaatg aactggaaat tttgtggggg caaccgatta aagacgacaa cgatcgcatc
    38461 agagctgtaa attcgctgca tcaacaaggt gttaaacgca tttttgttta tctgaaagat
    38521 gaatctgtat tttgcagcga taaggatgga gaacaatttt tgctgaccgc gccagcgcat
    38581 acgacagtag acagttttgg tgctgacgat ggttttatgg cgggcctggt atatagcttt
    38641 cttgaaggat acagtttccg cgacagcgcc cgttttgcgg tagcctgcgc ggcaatttcg
    38701 cgcgccagcg gcagcttaaa caaccctacc ctgtctgccg ataacgcgct ttcattagtg
    38761 ccaatggtgt aacaatgttg ccggattcga cgcttgaccg catcttatcc ggctcataaa
    38821 ttgcgcacta cgctaaacca ataaagaatc ctgcaatagt agcactcatc aggttagaaa
    38881 gtgtcgccgc cgccagcgcg cgtaaaccaa gttgggcgat ttccggcgca cggtgtggcg
    38941 caaccgcaga aaacgccccc accaccaccc cgatagaacc aaagttagcg aaaccgcaca
    39001 acgcgaaaga aataatcgcc acggttttag catccagagt gccacccgtt tgcagatatg
    39061 gcgagaaatt gagataagcg acaaattcat tgatcgccag cttctgccca atcaaactcc
    39121 cggcaagatt tgcatcactc cagtcaaccc ccatcaccca cgccaatggg gccaacaggt
    39181 aacctaaaat cgactccagc gaagcatgtt caaaaccaaa ccagccgcca acaccgccga
    39241 taataccgtt aatcaacgca attattgcaa caaatgccat aacaactgtc gctacaccgg
    39301 cggcgatttt cagcccggtc attgcccctg tcgcagcggc ttcaataatg ctttttggcg
    39361 gtgtttcggt gaaagagagg ttattaaaag aaacctgcga agattccgta gccgggctta
    39421 acaggcgggc aaacaagatc ccgccaggga tcgccattaa cgatgccgcc agcaaatatt
    39481 ccacaggtac gcccagtgcg gcgtaaccaa tcattgtcga accagcaatc gaggccatgc
    39541 cactacaaat cgctgtaaat aattcattgc gattcagacg atcgataaag ggtttgacga
    39601 tcgccggaat ttcgttttgc ccgaggaaaa tggtggtgac cgcgacgaat gactcgatct
    39661 tgctgatatt taaagctttc tggaagatac cgccgagaat gcgaattaag atccccatca
    39721 caccgatgta gtagagaata ctcaccagtg cagtgacgaa gataattgcc ggtaataccc
    39781 tgaaaccaaa gataaatcct gcgccatcaa ataaggtatc cattttcggt ccgaccagag
    39841 aaccgaagat aaatgcgcta cctgcgtcgc tgtacgccat cactttgtgg acgccaaaag
    39901 cgactttttc tgcgacccaa cgccctggcg gtaaccaaag cataatcccg ccaatcacga
    39961 cctgtaacac taacgccgcg ccaacggtac gcaggctgat cttcttcttg tttactgaca
    40021 gtaaaaacgc aattgccagc agtaccacca ttcccagaac acttctcatg acatccatag
    40081 tgattatctc ttcatgtcat tttgggcgac ggctttctgc ccatcagaaa tagccgggcc
    40141 tgcatcttgc gatactgtcc cggcttgtta tcaacactta atgggttttt atgtagccgc
    40201 gtacgcactc ttcgactaat ccccagaacc agtcggtatc aatagtgatg ccgactttag
    40261 tattggcggg cttgccaaga acgcccagct cgtcgcagac ggtacgccca tagcaagggc
    40321 cactgttgac gtccacttcg acgtacatct cctgggtttt aatgccatca gggttaatca
    40381 gataaccgat gcaggtggcg tcgtgcaccg ggccgccagc caggccgtag ttttcgaact
    40441 gcgttttgag agtgaagttc atgatgtcgc tgaacatctc tccggcaggg ccgcctgccc
    40501 tttccattcg tgcaattacg tccggggtgc aaacggtctg gttggtaaga tcgaggccca
    40561 tcatcactaa tggaacgccg gaggtgaaca ccacgcgtgc ggcttccggg tcggcaaaga
    40621 tattgaactc ggcagatggg gtgaagttgc ctgtaccgta agcgccgccc attagcacaa
    40681 tttcacggat tttgggcagg attgcgggtt gcatacgcat tgccaccgcg atatttgaaa
    40741 gcggaccaac cggcaccaga gtgatatcgc catcgcttgc cataagggta tcaatgatgt
    40801 atttcaccgc gtgggtactt tctgcctggc gggtcagcgg ctcgaatacc gggccatcca
    40861 gtccggtttc accgtgaata ttatcggcaa cgatttgttg acgcataatg ggttgcggca
    40921 tccccgcata aaccggaaca ttaatctcca gtttctggca aacattcagg ccattaatta
    40981 atgttttatc aagcgtctga ttacccgcaa caatggtgat acctaataaa tcaatcgccg
    41041 gatgtttcgc cgccatcatt atagcaatag catcatcatg acccggatca caatccagaa
    41101 taatttttct cttttccatt gtttatttcc tctgtttcca gttgcgttat tttttctaca
    41161 gcaacgaaat ttcgatcggg ccgatgattg aatcttaaca acagcgtacg aatgctaaat
    41221 atgagaaatc tcatagcgga taaacatcgt gaaagaaatc cacaataatg atcttaagca
    41281 gcaattgatg agtgaatccg cgtttaagga ttgcttttta acggatgttt cagccgatac
    41341 gcggctgttt cattttttag cgcgtgacta cattgtgcag gaagggcaac agccgtcctg
    41401 gctgttttac ctgacgcgag gccgcgccag gctttacgcc acgctagcta atggtcgcgt
    41461 gtcgctgatc gatttctttg ccgccccctg ttttattggc gagattgagt taatcgacaa
    41521 agaccatgaa ccgcgtgcgg tgcaggctat tgaagagtgt tggtgccttg cgctccctat
    41581 gaaacattac cgcccgctgt tattaaacga cacgctattt ttacgaaaac tctgcgtcac
    41641 cttaagtcat aaaaattatc gtaatattgt ttctttaact cagaatcaat catttccgtt
    41701 agtgaatcgc ctggcagcat ttatattact ctcgcaggaa ggtgatcttt atcacgaaaa
    41761 gcatacgcaa gcggcagagt atttaggcgt ttcttatcga catcttttat atgttctcgc
    41821 gcaattcatt cacgacggtt tattaattaa aagcaagaaa gggtatctaa ttaaaaacag
    41881 aaagcagttg tcaggactgg cgctggagat ggacccggag aataaattct ccgggatgat
    41941 gcagtaaaaa ttattggcaa tagcacgatt gccggatgca acgcttaata aggcgtacat
    42001 ccggcaatga aactcaagct aaaccaataa agaacccggc aatggtcgcg ctcatcaggt
    42061 tggaaagcgt cgccgccgcc agcgcccgta aaccaagctg ggcgatttcc ggcgcgcgtt
    42121 ttggcgaaat agccgaaaac gctccaacga caacaccgat agaaccaaag tttgcaaacc
    42181 cacagagggc gaacgagata atggcaatgg tcttgacatc cagcgtgccg ctcgtttgca
    42241 gatagggcga gaaattgaga taagcaacaa actcattaat cgccagtttc tgcccaatca
    42301 aacttccggc aagatttgca tcactccagt ccacccccat aatccatgcc aacggggcca
    42361 gcacatagcc gaaaatgctt tccagagaga cattggcaaa accaaaccag ccgccaactc
    42421 caccaataat gccgttgatc agcgcaatga tcgctacaaa tgccattacc accgtcgcca
    42481 caccggcggc gattttcagc ccggtcattg caccgttcgc cgcagcttca ataatgcttt
    42541 ttggcggtgt ttcgctaaac gacagatttt caaacgtaac ttgcgaaggt tccgtcgccg
    42601 gactaagaat acgtgcaaac aggataccgc caggaatagc catcagcgaa gcggctaaca
    42661 aatagtcaat cggcaccccc attccggcat agccaatcat catcgaaccc gcaatagacg
    42721 ccatgccgct acaaatagcc gtaaatagct cgttgcgatt catgcgatca ataaaaggtt
    42781 taacgatcgc cgggatctca ttttgcccga ggaaaatagt ggtgactgcg acaaaagatt
    42841 cgattttgct gatgttgagg gctttctgaa aaatactgcc aagaatacga atcagcagcc
    42901 ccattacgcc aatgtagtac agcaagctga taagcgcggt aacgaaaata atcgccggga
    42961 gtacgcgaaa ggcgaagata aagcccgcac cgtcaaacag aacatccatt ttgggaccga
    43021 ccagcgaacc aaaaataaag gcacttcctg cgtcactgta ggacatcact ttatggacgc
    43081 ctaatgccgc ctgctctact gcccattttc ccgggggaaa atagagcatg atgccaccaa
    43141 tggcaatttg cagcagcagc gcagcgccaa ccgtgcgcaa gctgatgctc tttttattta
    43201 ccgacaacac aaaggcaatt gccagcaata ccaccatccc cgcaacactt ctcattatat
    43261 ccataatgat tttcccttca tgccagtaaa cccggcgtca gcgccaggtt ttggtatgtt
    43321 tgatgagtac gggcgacggc tttctgcccg ccagaaatta acccacgaga cgctgatatt
    43381 ctttggcaat ttcgctcgcc agaatggcgt tattaaagac cagttgaata ttggatttca
    43441 ggctatcacc gccggttagc tccgcaacac gcgccagcag gaacggtgta ctctctttcc
    43501 caataacacc ctgttcttcg gcttcagcaa cggcttgatc aattgctgca ttgatggttt
    43561 cttctggcat cgcaaactgt tccgggatcg ggttcgctac caccaggccg ccgttcaaac
    43621 cggtttgcca cttcactgcc atcgcgcgag caatttcgcg ggcactgtca agacgaatgc
    43681 tgacttcaaa tgggctggtt cggcagaaaa acgcaggcag cgcttttgtc tgataaccaa
    43741 ttaacggtac accgaaggtt tctaaatatt ctgttgttaa tcctaaatcg agaatagatt
    43801 ttgcccccgc acaaacaaca gtgacattgg tatttgccag ttcctgtaaa tcggcagaaa
    43861 tatcgaaggt atgttccgct ccgcgatgca caccaccaat acctccggtt gcaaaaactt
    43921 taatcccagc cagtgctgca ataatcatgg ttgatgcaac ggtagtcgcg ccatttttcc
    43981 ctgcagcaac gacaaaaggt aaatcacgac gactaacttt cgtcacgtta tgtccttcac
    44041 ggcccagcaa ttcgatttct tctttactta agcccacttt catcacaccg ccaataatgg
    44101 cgatagtggc tggtactgcg ccctgtttac gaattgtttc ttctacttca attgcggttt
    44161 gggcattttg tgggaatggc attccgtgag aaattatggt cgattccagc gcaacaaccg
    44221 gttttttatt ttttaaagca tcctgtactt cagcggatat ttgtaataat tcaggggaca
    44281 gagttaattc agacattctg tgttctccac taacgatcta acattggcaa ttgataattc
    44341 agggttgttg gtatattcac aggcaagtgc cattgacgaa catccctgcg caaatcgaac
    44401 agaatcgata aacggcatac cgtctaccca acaggaggcc agccccgcca tcatggcgtc
    44461 gcctgcccca gtaacattga tgacattcgt tttaataggg gccgaccacc cactttcacc
    44521 gttgatatca ctgtagtaaa cgccatcccc tcccatgctc agcaccaagc ggttcaggcc
    44581 gtgttgatga aaccaggcgg cgacctttgc gacatcttca cgtccggaaa gtgcaattcc
    44641 gctcaacgtt tcggcttcaa gacggttagg ttttaacgta tggattttgc tcagatgatc
    44701 gcggattttg acgcatttcc atgccgatac cggatcaata aatacaggcg tttccccgct
    44761 attttccaga acccagacca gcacctcttc actaaggttg caatccgcga caataacctt
    44821 tgcaccgcga ataaactcct gatgctgggc aagaaattct gcagaaatac agtcactaat
    44881 actcatatca tttatcgcga caagcatttc tcctgtatta tcgagcagtg ataaatagct
    44941 ggatgtattc tcacccggaa caattaagca tttatcgaca taaacaccgg attgattagt
    45001 ttgcgccagt aatgattgcc cataaaaatc accacctact gcacttaaaa gccaggcatt
    45061 tttccccagt aacgccagat tatgagcgat attccttccc acgccacctg gggtaaattt
    45121 aatttttccg ggattcgaat ccgcataatt caatggatga tgtgaatacc cagccacatc
    45181 aatattcgcc gaaccaataa ttactatgta gtccttttcg cgcatagcac gccccctggc
    45241 aataaattgc caacgaagaa attgggtaaa atagccatta cagtcaataa gactattaga
    45301 gcacgtgttc agatttaaac acatgttcat aataagcata tttaacgtaa tgtaaatcga
    45361 tcagaaaaaa cgaaatcggc cagatatgaa ggcggtcaca aagtttgtct atttatgcag
    45421 caacaaaaga gaaagttgtg atgtgtaaca cacaatatgg caggcaagaa aaagggcaga
    45481 gatgtttctg ccctccagat tgttgacaac gcgcgctttg ttcatcctgg atgtggcgta
    45541 aacgccttat ccgacctaca aaagcgcaaa aaattcaata tattgcaggg ttcatgtagg
    45601 tctgataagc atagcgcatc aggcaatttt gcgtttgtca tcatcggggc agagatgtct
    45661 catccccgat aatgaagata aattatgcgt ttttttctac tatttgtgtt tccgggcgtt
    45721 tcaggaaggc ataggccaaa cccgccacca gcgtaccggc aataattgct accaggtaac
    45781 ccaataccgg cgtaatagcg ccagggatca gcagaacaaa cagaccaccg tgtggtgcca
    45841 tcagtttcgc accaatcgcc attgagattg cgccagtcag cgccccacca acgatacagc
    45901 acggtagcac acgcatcgga tcacgagcag caaacggaat tgcaccttcc gaaataaagc
    45961 acagaccaag taccagagcg gctttgccac cttcctgctg cgctttgtcg aatttgcgac
    46021 gcgccaccat tgttgccaga cccattgcca gcggtggcac catacctgcc gccataatcg
    46081 ccgccatcgg gccataggtt tgagtactca gcagacccac accgaatgcg tacgctgctt
    46141 tgtttaccgg accgcccatg tcagtacaca tcatgccacc gaggatcgcc cccagcagaa
    46201 ccgcattcgc agtccccatg gtctgcagcc agtgagtcaa cccatcgaga atgccagcaa
    46261 ccggtttacc gattaggtag atcatcgcca gaccgaccac cagactggaa attagcggaa
    46321 tgatcaggat cggtttcagc gcctccatac tctgtggcag tttcaattgc gtactgatta
    46381 acttcgcaat gtaaccagcc aggaagccag caattatacc gccaataaag ccagaaccgg
    46441 tgctgaccgc cagcataccg ccaatcagac ccggtgtgag gcccggacga tcggcaatgg
    46501 agaaggcaat ataacctgcc agtaccggca ccatcagcgc aaaggctgaa ccaccaccaa
    46561 tctgcatcag cgccgctgcc aacgtacccg gctctttaaa cgcttcgata ccaaaagcaa
    46621 aagaaagcgc gatacacaga ccacctgcaa caaccatcgg cagcatgtaa gagacgcccg
    46681 tcagcaagtg acggtaagcg cctgcactct ctttcttacc ttcagtggtc gctgtttgag
    46741 ctttgcccgc tggttcatac ggcgttgctt cagcaaccgc tttatccagt tcctgcgcag
    46801 ttttcttcag cgccagaccg gtagaggtgc gatacatcgg tttaccagca aatttcgcca
    46861 gatccacttc gatatctgcc gccacaatca ccagatccgc tgctgcgact tcttcagggg
    46921 tgattgcatt gcccgcgcca acagaaccac gggtttcaac tttcacccac cagccacgtt
    46981 ttttcgcttc ggtttcaatg gcttcagccg ccataaaggt gtgtgctacg ccagtcgggc
    47041 aagcagtcac cgcaactacg cgtttcggac cgctggcagc aaccggtgct gtcgcagcga
    47101 ccggcgcagt gtaaggtttc gcatggcctt tggcttcact caggaacagc tcagggtgcg
    47161 caactgcccg ggaaatatca cccagccaga catttttacc gttcagcgcg ctgtcattcg
    47221 ggatggaatc accgagaaca atcgccattt ctgcgtcgtt cggattgtcg atgatttcca
    47281 gttttgcttt tcgcgccgcc gcgcccagca gggtcttcgc catataggcg cgtgcctgac
    47341 cgagattagc gtcaataatc agcagcgttt tcattatgcc tctcctgctg tcagttaaaa
    47401 ggttgtaagt cgacgcgcgc catcattgcg gccaactgcg gacgatcggt aatacccaca
    47461 ttgctttgac ttaccgccag ggctgcaaca gctgtcgcca gacgcagtgt gtgttcactg
    47521 gattcacgca tcagcaagcc ataaatcagg ccaccaacca tagaatcccc tgcgccaacg
    47581 gtgcttacga catcgactga cggtggtttg gcgatccatt cgccggaggc attaacccaa
    47641 agcgcgcctt cggcacccag tgaaataaca acatgcgcaa taccttgttc acgtagcgca
    47701 tgtgcagctt caatcacatc tttcatttca ggcagtttac ggcctgccca gatttccaac
    47761 tcgcggcggt taggtttcac cagccacggt gccgctttca aacctgctac taacgcttca
    47821 cggctactat caaagataat gcaaggacac tgactacgca ggcgagtcat ccagtcggtg
    47881 aacgcttccg ggctgacgcc tgacggtaag cttccgctga cacagaccat atcgaactga
    47941 ccgagccagc tcagagaatc agtcacaaag cgttcccagt cggcgagggt gacttcaaaa
    48001 cccgagaagt tgaagtcggt cacttcgccg tctttttccg tcagcttaac gttaattcgg
    48061 gtgcgcccct gtacaacctg gaaacggttg gcaatgccca gctcgctgaa cagttgctga
    48121 aaaccatcct gattgtcttt acccaggaag ccgccaacgg tgacatcaat tcccaggtct
    48181 tttaatactt tggccacgtt gatgccttta cccgccgcat gcagaccggt ggttttcacc
    48241 aggttcactt cgccgcgttc aatttccggg cagaaaccaa caaggtcata agccggatta
    48301 agggtgatcg tagcaacacg tctgctcatt atgcgccctc cccaagacca gcagcgatag
    48361 cgtcgccaat ggctttcagc gcctgttcag catctgcacc ctgggcggta aagcgtaggc
    48421 gatgaccttt cttaacgcca agtgccacaa ctttcatcag actacgtccg tttgccggtt
    48481 tgccggtgcc atcaaggttt gtcacggtaa tatcactgtt aaattgttta atggtattga
    48541 ccagcatggt acctggacga gcatgcaggc cgtgttcatt gcgcaccaca aactccgcgc
    48601 ttaacacgtc gtcggtcggc gcatcatcgc tggtcagcag cgccagcaac gttgccgcat
    48661 ccgctttcag caagcggtca gctttattgt cgagcaataa atcagcgaga cgcttaagaa
    48721 ccgcgatggg ctgatcgtca ttcatcgcca cactcaccag catggctgcc gtttcgccgt
    48781 ccacatcaaa agcatttgcc gcacggctta ccgcaatcgc gctacgcaga ttgccttcgg
    48841 cgctatcgct cagccagata ccctgtccga gattcagcgg ttgttcattg atggctttgg
    48901 tgacgaaagt ggcgtcaact gcccccacct ctttcagacg cgcagcgttc agcgcctgaa
    48961 gagtcagcag atcgctggcg acgatatcca gcgtcagcat ttcgttgtcg agcttcagct
    49021 gctcactctg cttttcgccc atcagtaatg cgcgaagttc ttctgctgtt gttgctgact
    49081 tcagttgttc agcaacggaa tcatcgctca gtacgtgggt cagctggcgt agcaggccca
    49141 gatgttcatc cgagctggca gcaataccga ttgccacgta cgctacctga ccgtcacccc
    49201 aggtgacgcc ttccgggaac tgaaatacct gaacgccggt tttcagcacc tgatcgcggg
    49261 tgtcggtagt gccgtgtgga atagcaatac cattgccgag gaacgttgag gtttgctgtt
    49321 cgcgcgccag catgccattg acgtagcctt ctgctacatt accggcctgc accagcgccg
    49381 cagcgacctg gcgaatcgcc tcttctttgt ctccggcctt ttcgcccgga tggatgtcct
    49441 gtacggataa ctggaacata gttctcctct cttgctgaat tgaaacgatt cagcctctat
    49501 gagaaaaaaa gcgccaacct ggcttagggt taaagacaag atcgcgctga aacgtttcaa
    49561 gaaagcataa tacttctgtt tcagcacgca aggaaagccg taaagttatg agcaaaagtt
    49621 tgatgtgctg cacaattttt tgtcattttt cctaattgct gatgggaaaa tccgttatca
    49681 gcaatttcat ttcagcagcg tcagcagcca atcagcaaca gtgaaatggc attttgatta
    49741 ttcgtggcgc ttttgatttc gctgtctgtt tattttctga caagcagcgt aaactccgcg
    49801 tcttcctctt ccagtgatcg acctgcatgc ataactcccc cgcagtctcc agcgcgaaat
    49861 cgtttgacct gacctcgacg gcgtttttaa tcgttgcctt tctcaccggt attgcgggtg
    49921 ctctgcaaac cccgacactc agtattttcc ttaccgatga agtacatgcc cgtccggcga
    49981 tggtgggatt cttctttacc ggcagcgctg tcattgggat tctggtaagt cagtttctcg
    50041 ctgggcgctc tgataagcgc ggcgatcgca aatcgctgat tgtcttttgc tgcctgttag
    50101 gcgtgctggc ctgcaccctt tttgcctgga atcgcaacta ctttgttttg ctattcgttg
    50161 gcgtctttct tagcagcttt ggctcgaccg ctaacccgca aatgtttgcc cttgcccgtg
    50221 aacatgccga caaaaccgga cgtgaggcgg tgatgttcag ctctttttta cgcgctcagg
    50281 tttcactggc atgggtcatt ggcccaccgc tggcttatgc gttagcgatg ggtttcagct
    50341 ttacggtaat gtatctgagc gcagcggtag cgtttattgt ttgcggcgtg atggtgtggc
    50401 tgtttttacc gtcgatgcaa aaagagcttc cgctggcgac cggcacggtt gaagcgccgc
    50461 gccgtaaccg tcgcgatacg ctactgctgt ttgtcatttg tacattgatg tggggctcga
    50521 acagcctgta catcatcaac atgccgctat ttattatcaa cgaactgcat cttcccgaga
    50581 aactggccga tgtgatgatg gggaccgccg ccgggctgga aatcccgacg atgttgattg
    50641 ccggatattt cgccaaacgt ctgggtaagc gtttcttaat gcgcgttgct gccgtgggtg
    50701 gcgtctgttt ttacgcagga atgctgatgg cgcattcacc tgtcattctg ttgggcttgc
    50761 agctgctaaa tgctattttt attggcattc tgggcggcat cgggatgctc tattttcagg
    50821 atctgatgcc cggtcaggcg ggttcagcca ccacgctcta taccaacact tcgcgcgtgg
    50881 gctggatcat cgcaggatca gtggcgggca tcgtcgccga aatctggaat tatcacgctg
    50941 tgttctggtt tgcgatggtg atgattatcg ccactctgtt ttgcttactg cggattaaag
    51001 atgtttaagg cgcggtcagc atttccagat cgataagcca ggtcatggcc tgctgacgcg
    51061 agttaccgca catttcagcg ctggcttgta agcctgcaca gactttcggt cgcagtggcg
    51121 aggtgaaaat tttacagcgt tgctgttcat caagctgaat gcagggcgta ttggcgggct
    51181 tgccatctgg cataccggga atagggctgg aaattgaagg ggcagtgcaa cacgccccgc
    51241 aacccggacg gcattccata cgttgttctc ttaaggttaa ccaccggatt gatgagcgcg
    51301 cacagtacca ccttttttgc accagcaaaa gtgcgaatac cacttgcccg aaaggcccgt
    51361 cgcgagtact ttgtcgcgat atttttgaca ttttcgacta caggaatttt tcgatgccaa
    51421 gagcgaacga aattaaaaaa ggtatggtac tgaattacaa cggcaaactg ctgctggtta
    51481 aggatattga tattcagtcg cccactgccc gcggtgccgc tacgctgtac aaaatgcgtt
    51541 tttctgatgt ccgtaccggg ctgaaagtag aagagcgttt caaaggtgat gatatcgttg
    51601 acaccgtgac gctgacccgc cgttacgttg acttctccta tgtcgatggc aacgaatatg
    51661 tctttatgga taaagaagac tataccccgt ataccttcac caaagatcag attgaagaag
    51721 agttgctgtt tatgccagag ggcggcatgc cggacatgca ggtgctgacc tgggacggtc
    51781 aactgctggc gcttgagctg ccgcagaccg ttgatctgga aatcgttgaa accgcaccag
    51841 gcatcaaagg ggcatctgcc agcgcccgta acaaaccggc gacattgagc actggtctgg
    51901 tgattcaggt accagaatac ttaagcccgg gcgaaaaaat tcgtatccat atcgaagaac
    51961 gccgttatat ggggcgtgct gactaacttc agccgcatgc agaaaaggga tagctcaggc
    52021 tgtccctttt ttaatttatt ataccaatct tctattttgc gcttcctgaa caccttacgc
    52081 cctcaatttt cactcgttga ttgatcgccc tcacacttca tcgcattaac aatccagacc
    52141 aatttcaatt gctgtcatat aactttacac tgtcattgtt aatttatcgt tactaagacg
    52201 tgactcctat gaagacaatt gcctccgtta cgctcccgca tcatgtacac gctccacgct
    52261 acgatcgcca gcagttgcaa tcacgtatcg ttcattttgg ctttggcgcc tttcaccgcg
    52321 ctcatcaggc gttactgacc gatcgtgtgc tgaatgccca gggcggcgac tgggggatct
    52381 gtgaaatcag cttgttcagc ggtgatcaac tgatgagcca gctccgcgca cagaaccatt
    52441 tatataccgt gctggagaaa ggtgcggacg gcaatcaggt gataattgtc ggtgccgttc
    52501 acgaatgcct taatgcaaaa ctggattcct tagcggcaat tattgagaaa ttttgcgagc
    52561 cacaggtggc aattgtttcc ctgacgatta ccgaaaaagg ctattgtatt gacccggcca
    52621 ccggtgcact cgacaccagt aatccgcgga ttattcacga tctacaaacc cctgaagaac
    52681 ctcactccgc accgggtatt ctcgtcgaag cactgaaacg ccgccgtgag cgcggcctta
    52741 caccgtttac cgtgctctcc tgcgacaata ttcccgacaa tggccatgtg gtgaaaaacg
    52801 cggtgctggg aatggcagaa aaatgttcgc cagaactcgc cgggtggata aaaaaacacg
    52861 tcagttttcc gggaaccatg gtcgaccgca ttgttccggc tgcaaccgac gaatcactgg
    52921 cagaaatcag ccagcatctg ggggtaaatg atccctgcgc gattagctgc gaaccgttta
    52981 tccagtgggt ggttgaagat aacttcatcg ctgggcgtcc tgcctgggaa gtcgcaggtg
    53041 tacaaatggt gaatgatgtc ctgccatggg aagagatgaa actgcgtatg cttaatggca
    53101 gccactcttt tctcgcttat ctgggttacc tctcaggatt cgtccatatc agtgattgca
    53161 tgcaggatcg cgtatttcgc catgctgcca gaacattaat gctggatgag caagcgccga
    53221 cactgcaaat taaagatgtc gatttaacac aatatgcgga taagttaatt gcacgttttg
    53281 ctaatccgtc gctgaaacat aagacctggc aaatcgcgat ggatggcagc cagaaattac
    53341 cgcaacgtat gctggcaggt attcgcatac atctggggcg tgaaacggac tggtcgttgc
    53401 tggcattagg cgttgctggc tggatgcgtt acgtcagcgg cgttgatgat gccggaaatg
    53461 ccattgatgt tcgcgatccg cttagcgata aaattcgcga acttgttgcg ggcagcagca
    53521 gtgaacaacg cgtaaccgcc ctgctttccc tgcgtgaagt tttcggtgat gatctgccag
    53581 ataacccgca ttttgtgcag gccatcgaac aagcctggca acaaatcgta caattcggcg
    53641 cacatcaggc gctattaaac accctcaaaa tttaacgatt tctgcggtta aagcggatga
    53701 agctcacctt cgtccgctct ccccttctct tttctgcctt ttttagccag gattaacgct
    53761 cagttaactt accagagtat tgcggagccg ttgtgaccag gaccaacctc atcaccggtt
    53821 ttctcggcag cgggaaaacc acgtcgattc ttcatctgtt agcccataaa gatcccaacg
    53881 aaaaatgggc ggtactggtt aatgaatttg gggaagtcgg aattgatggt gctttgctcg
    53941 ccgatagcgg cgcattgctg aaagagatcc ccggcggctg catgtgctgc gttaatggtt
    54001 tacccatgca ggtagggttg aataccttac tgcgtcaggg aaaaccagac cgcttgttga
    54061 tagagccgac cgggctgggc catccgaaac agatcctcga tctgttaacc gcaccagtct
    54121 atgaactgtg gatagatctg cgcgccacct tgtgcattct cgatccacgc ctgctgctgg
    54181 acgaaaaaag cgccagcaat gaaaacttcc gtgaccagct ggctgccgca gacatcattg
    54241 tcgccaataa atccgaccgt gcgacgcccg aaagtgagca agcgctacag cgttggtggc
    54301 agcaaaatgg tggcgatcga caattaattc acagtgagca tgggaaagtt gacggtcatc
    54361 ttctggattt gccgcgtcgc aatttagccg agttgcccgc cagcgccgcg cattctcatc
    54421 agcatgtcgt gaaaaaaggg ttagcagcgt taagcctgcc agagcatcaa cgctggcgtc
    54481 gcagtctgaa cagcgggcaa ggatatcagg cctgcggctg gatattcgac gctgatacgg
    54541 tattcgacac cattggcatt ctggaatggg cgcgacttgc accggtggaa cgcgtcaaag
    54601 gcgtgctgcg tattcccgaa gggctggtac gaatcaaccg tcagggcgat gacctgcaca
    54661 ttgaaacgca aaacgttgcg ccaccggaca gccgtattga gctgatttcc agcagcgaag
    54721 ctgactggaa tgctttgcag agcgcgctgt tgaagcttcg tttagcgact accgcgtaag
    54781 gttgcctgcg tttttcagta agataattag agaaaatatg attaaaaatt tgccgcaaat
    54841 agtgttgttg aatattgtcg gcctcgcgct gtttctttcc tggtatattc ccgttaatca
    54901 tggattctgg ttgccgattg atgcggatat tttttatttc tttaatcaga aactggtcga
    54961 aagtaaggcc tttttgtggc tggttgcatt gaccaacaat cgcgctttcg acggttgttc
    55021 tctgctggcg atgggaatgt tgatgctgag tttctggctg aaagaaaacg cccctggcag
    55081 acgacgtatc gtgattatgg gtctggtcat gctattaact gcagtggtat taaaccagct
    55141 gggtcaggca ttaattcctg taaaacgggc cagcccaaca ttgactttta ccgatattaa
    55201 ccgcgtcagc gaactgctct ctgttcccac gaaagatgcc tcacgagata gctttcccgg
    55261 cgatcacggc atgatgctgc ttattttttc ggcattcatg tggcgttatt tcggcaaagt
    55321 tgcaggcctt atcgccctta ttatttttgt ggtttttgca tttcccagag taatgattgg
    55381 cgcacactgg tttactgaca tcattgtcgg ttcgatgacc gtgatattga tcggtttgcc
    55441 ctgggtgttg ctgacgccat taagtgatcg attaatcacc ttttttgaca aatcactacc
    55501 gggaaaaaac aaacatttcc aaaacaaata actcacagta attaacatca tcaggcttat
    55561 ttttatagtg aggataatcc tgatgatgcg caccgtgctt tcatccatcg cgcgcaaaaa
    55621 tcattctcta agtaaatgaa tggattgcat gcgtttcact caatcgtact ttaattgacc
    55681 aatcccgctt attaactttc tgtatcactt tttcttataa aaaatcatgt aaaaccgctc
    55741 gccaagaccg caccaatcgg gtaatctaga actcgttttg cctcggcggt agattatcct
    55801 cacagcatat aattttgtgc gttagtccac agatttggcc ttaaggaatt gtttcaacat
    55861 gcccaggtaa ttagtctcgt gtcgcttggc atttttttat aacgatattt gtcgttaagg
    55921 acttcaaggg aaaacaaaca acatggtcaa atctcaaccg attttgagat atatcttgcg
    55981 cgggatcccc gcgattgcag tagcggttct gctttctgca tgtagtgcaa ataacaccgc
    56041 aaagaatatg catcctgaga cacgtgcagt gggtagtgaa acatcatcac tgcaagcttc
    56101 tcaggatgaa tttgaaaacc tggttcgtaa tgtcgacgta aaatcgcgaa ttatggatca
    56161 gtatgctgac tggaaaggcg tacgttatcg tctgggcggc agcactaaaa aaggtatcga
    56221 ttgttctggt tttgtacaac gtacattccg tgagcaattt ggcttagaac ttccgcgttc
    56281 gacttacgaa cagcaggaaa tgggtaaatc tgtttcccgc agtaatttgc gtacgggtga
    56341 tttagttctg ttccgtgccg gttcaacggg acgccatgtc ggtatttata tcggcaacaa
    56401 tcagtttgtc catgcttcca ccagcagtgg tgttattatt tccagcatga atgaaccgta
    56461 ctggaagaag cgttacaacg aagcacgccg ggttctcagc cgcagctaat aaaccgtttg
    56521 gatgcaatcc cttggctatc ctgacgagtt aactgaaagc actgcttagg cagtgctttt
    56581 ttgttttcat tcatcagaga aaatgatgtt tccgcgtctt gatccaggct atagtccggt
    56641 cattgttatc ttttaaatgt tgtcgtaatt tcaggaaatt aacggaatca tgttcatacg
    56701 cgctcccaat tctggacgta agctcctgct tacctgcatt gtagcaggcg tgatgattgc
    56761 gatactggtt agctgccttc agtttttagt ggcctggcat aagcacgaag tcaaatacga
    56821 cacactgatt atcgacgtac aaaagtatct cgatacctat tttgccgacc tgaaatccac
    56881 tactgaccgg cttcagccgc tgaccttaga tacctgccag caggctaacc ccgaactgac
    56941 cgctcgcgcg gcgtttagca tgaatgtccg tacgtttgtg ctggtgaaag ataaaaaaac
    57001 attctgttca tctgcgactg gtgagatgga cattccactc aatgaattga ttccggcgct
    57061 caacattaat aaaaacgtcg atatggcgat cttacccggc acgccgatgg tgccgaacaa
    57121 acccgcaatc gtcatctggt atcgcaaccc tttgctgaaa aatagcggtg tgtttgccgc
    57181 tctgaatctc aacctgacgc cttctctctt ttatagttca cggcaggaag attacgatgg
    57241 cgtcgccctc attattggca atactgcgct atctaccttt tcttcacgtt tgatgaacgt
    57301 taatgaatta accgacatgc cggtccgtga aactaaaatt gcgggcattc ctctgaccgt
    57361 tcggctttat gcggatgact ggacatggaa cgatgtgtgg tacgcatttt tactgggcgg
    57421 catgagtgga actgtcgttg gcctgctctg ctattacctg atgagcgtac gtatgcgccc
    57481 cggcagagaa atcatgaccg ccatcaagcg cgaacaattt tacgtggcgt atcaaccggt
    57541 ggtggataca caagctttgc gagtaacggg cctggaagta ctgctacgct ggcggcatcc
    57601 tgtcgcggga gaaattcccc cggatgcctt cattaacttt gccgaatcgc aaaagatgat
    57661 tgtgccgctg actcagcacc tgtttgagtt aattgcccgc gatgccgcag aattagaaaa
    57721 agtgctgcca gtaggcgtca aatttggtat taacattgcg ccggaccatc tgcacagcga
    57781 aagctttaaa gcagatatcc agaaactgct cacttccctg cccgcacacc atttccagat
    57841 tgtgctggaa attaccgagc gcgatatgct gaaagagcaa gaagccacac aactcttcgc
    57901 ctggctgcac tcggtcggcg tagaaattgc tattgatgac ttcggcaccg ggcacagcgc
    57961 gcttatctat cttgagcgtt ttacgctcga ttatctgaaa attgaccgtg gatttatcaa
    58021 cgccatcggt acggaaacga tcacttcacc cgtacttgac gcggtgctga cgctggcgaa
    58081 acgcctcaat atgctgacgg ttgctgaagg ggtcgaaacg ccggaacagg cgcgatggct
    58141 aagcgaacgc ggcgttaatt tcatgcaagg ctactggatt agtcgccctt taccgctgga
    58201 cgattttgtc cgctggctga agaaaccgta tacgccgcag tggtaaggtg tgcttacgtc
    58261 ccttattatt catagtgaaa gcatgccgga ttgcggctaa tgatgagtaa aaggaaatcc
    58321 attgcagatg attgtgcgca tactgctgct gtttatcgct ctgttcacct ttggtgtgca
    58381 ggcgcaggct atcaaggaaa gctatgcctt tgccgtgctg ggcgaacccc ggtacgcgtt
    58441 taatttcaac cattttgatt atgtgaaccc cgccgcgcca aaaggtgggc agataacgtt
    58501 gtcagccctc ggcaccttcg ataatttcaa ccgctatgca ctgcgcggca acccgggcgc
    58561 acgcaccgag cagctgtacg acacgctatt tacgacttcc gatgacgaac caggcagtta
    58621 ttacccgctg attgctgaaa gcgcacgcta tgctgacgat tattcctggg tggaggtcgc
    58681 tattaatccg cgcgcccgtt ttcatgatgg ttcgcccatt actgcccgcg atgtagagtt
    58741 tacttttcaa aaatttatga ccgaaggcgt gccgcaattt cgtctggtct acaaaggcac
    58801 caccgtcaaa gccattgcac cgttaaccgt gcgcattgag ttagctaaac ccagcaaaga
    58861 agatatgctg agtctgtttt cgctgccgat atttccagaa aagtactgga aggatcacaa
    58921 acttagcgac ccgctcgcca cgcctccgct tgccagtggt ccgtaccgcg ttacgtcctg
    58981 gaaaatgggg caaaatattg tctattcccg cgtgaaagat tactgggcag caaacttacc
    59041 ggtaaaccgt ggacgctgga atttcgacac cattcgctac gattattacc tcgatgataa
    59101 tgtcgccttt gaagcgttta aagcaggtgc ctttgatttg cgtatggaaa acgacgctaa
    59161 aaactgggcc acgcgttata ccggtaaaaa tttcgataaa aaatacatca tcaaagatga
    59221 gcaaaagaac gaatcagccc aggatacgcg ctggctggcg tttaatatcc aacgtccggt
    59281 attcagcgat cgccgggtcc gggaagcaat cactctcgcc tttgactttg aatggatgaa
    59341 caaggcgttg ttttacaacg cctggagtcg cacgaacagt tattttcaga ataccgaata
    59401 cgcggccaga aattaccccg acgccgcgga gctggtgctt ctggcaccaa tgaaaaaaga
    59461 tctaccgcca gaagtcttca cacaaatcta ccagccgccg gtatctaaag gcgatggcta
    59521 cgatcgtgac aacctgttaa aagccgacaa acttctcaac gaaacgggct gggtgctgaa
    59581 gggtcagcaa cgcgttaatg ccacaacggg tcagccgctc agctttgaat tattgcttcc
    59641 cgcaagcagc aatagtcagt gggtattgcc gttccagcac agcctgcaac ggctgggtat
    59701 caatatggac attcgcaagg tggataactc gcaaatcact aaccgcatgc gcagtcgcga
    59761 ctatgacatg atgccgcgcc tatggcgggc gatgccgtgg cccagttccg atttacagat
    59821 ttcctggtca tcggaatata tcaattccac ttataatgcc cccggcgtgc aaagcccggt
    59881 tatcgactcg ctgatcaacc aaattattgc cgcgcaggga aataaagaaa aattactgcc
    59941 gttggggcga gcactggatc gcgtattaac gtggaattat tacatgctgc caatgtggta
    60001 catggcggaa gaccgtctcg cctggtggga taaattctcc caacccgctg tacgccctgt
    60061 ttacagcctg ggtatcgata cctggtggta tgacgttaat aaagcggcca aactgccgtc
    60121 agccagacaa cagggagagt agatgggcgc ttacctgatt cgccgtctgt tgctggtgat
    60181 cccaacgtta tgggctatta tcactatcaa ctttttcatc gtgcaaattg cgcctggcgg
    60241 tccggtcgat caggccatcg ccgccattga gtttggtaat gccggagtat tacccggcgc
    60301 aggcggtgaa ggtgttcgtg ccagccatgc gcaaacgggt gtcggcaata tcagcgacag
    60361 taattaccgt ggcggacgcg gattagaccc agaagtgatc gctgagatca ctcatcgcta
    60421 cggtttcgat aagccgatcc acgaacgtta cttcaaaatg ctctgggact acatccgctt
    60481 tgattttggt gatagcctgt ttcgcagcgc ctcggtgctg acgctgatta aagacagtct
    60541 gccggtttcc atcaccctcg gattgtggag cacgctgatt atctatctgg tgtcgattcc
    60601 gttaggcatt cgcaaagctg tttataatgg gagtcgcttt gacgtctgga gtagcgcatt
    60661 tatcatcatc ggctacgcca ttccggcctt tttgtttgcc atcctgctga ttgtcttctt
    60721 cgcgggcggc agctatttcg acctgttccc tctacgcggc ctggtttccg ctaactttga
    60781 ttcgctgccg tggtatcaga aaatcaccga ttatctgtgg catatcacgc taccggtgct
    60841 ggcgacagtg attggtggct ttgcggcgct gaccatgctg accaaaaact cattccttga
    60901 tgaagtgcgc aagcaatacg tggtgaccgc gcgtgcgaaa ggggtaagtg agaaaaacat
    60961 tctctggaaa catgtgttcc gcaacgccat gctgctggtg attgccggtt ttccggcgac
    61021 gtttatcagc atgtttttta ccggctcgct gctgattgag gtgatgtttt cactcaatgg
    61081 tcttggctta ctgggctacg aagcgaccgt ctcgcgcgat tatcctgtaa tgtttggtac
    61141 cttgtatatt ttcaccctga ttggcctgct gctgaatatt gtcagtgata tcagctatac
    61201 gctggttgat ccgcgtatag attttgaggg acgttaatgt cgcgactcag ccccgtcaat
    61261 caggcccgtt gggcgcgttt tcgtcataac cgtcgcggct actggtcgtt atggattttc
    61321 ctcgttttgt ttggtttgag tttgtgttct gaacttatcg ctaacgataa accgttgctg
    61381 gtgcgttatg acggcagttg gtatttcccg ttgttgaaaa actacagcga aagcgatttt
    61441 ggcggcccgc tggcaagtca ggctgattat caggacccgt ggctgaaaca acggctggaa
    61501 aataacggct gggtactgtg ggcaccgatt cgctttggtg ctaccagtat caactttgct
    61561 accgataagc ccttcccttc tccaccctcc cggcaaaact ggctgggaac ggatgccaac
    61621 ggcggcgatg tgctggcacg cattctctat ggcacgcgga tctcggttct gtttggcctg
    61681 atgctgactc tctgttccag cgtgatgggc gtgctggcgg gggcgctaca aggctattac
    61741 ggcggtaaag tcgatctctg gggacaacgc tttattgaag tatggtcggg gatgccgacg
    61801 ctgtttttga ttattttact ttccagcgtc gtacagccta acttctggtg gctgctggca
    61861 attactgtct tgtttggctg gatgagtctg gtcggcgtgg tgcgggcgga gtttttacgt
    61921 actcgtaatt tcgactacat tcgtgcggca caggcgcttg gcgtcagcga tcgcagtatc
    61981 atcctgcgtc atatgttgcc taatgccatg gtcgcgaccc tcaccttttt accgtttatt
    62041 ttatgtagtt cgataaccac cctgacctcg ctcgatttcc tcggcttcgg tctaccgctc
    62101 ggttcaccgt cactcggtga actgctgtta caagggaaaa ataaccttca ggccccgtgg
    62161 cttgggatca ccgccttctt gtcggtggcg atattattgt ctttgctgat ctttattggt
    62221 gaagccgtcc gcgacgcatt tgatcctaat aaggcggtgt agcatgacgc aaactctgtt
    62281 agcgattgaa aatttgtcgg tgggttttcg ccatcagcaa accgtacgta cagtagtcaa
    62341 tgatgtttca ctacagattg aggctggcga aacgctggcg ctggtgggtg agtcaggttc
    62401 aggcaaaagc gttaccgcgc tgtcaatttt acgcctgctc ccttccccgc cggttgaata
    62461 tctctccggc gatattcgtt ttcatggcga atcgctgctt catgccagcg atcaaacgtt
    62521 gcgcggtgta cgcggtaata agatcgccat gatttttcag gaaccgatgg tgtcgttaaa
    62581 tccattgcat accctggaaa aacagcttta tgaagtgctt tcactccacc gcgggatgcg
    62641 tcgggaagcg gctcgtggcg aaattcttaa ctgccttgat cgcgtcggta tccgccaggc
    62701 ggcaaaacgg ctgacagatt atccgcatca gctctccggc ggcgaacgcc agcgggtgat
    62761 gattgcgatg gcgctgttaa cgcgaccgga attattaatt gccgatgaac cgaccaccgc
    62821 actggacgtc tctgtccagg cgcagatttt acagctgttg cgcgaactgc aaggcgagct
    62881 gaatatgggc atgctgttta ttactcataa cctcagcatt gtcagaaaac tggcccaccg
    62941 cgtggcggta atgcaaaacg gtcgctgtgt cgagcaaaat aacgccgcta cgctatttgc
    63001 ctcacccact catccttaca cacaaaagct actcaacagt gaaccgtcag gcgatccggt
    63061 accgttgcca gaacctgcct caacgttgct ggatgttgaa cagcttcagg ttgccttccc
    63121 cattcgcaaa gggattttga agcgcattgt ggatcataat gtggtggtga aaaacatcag
    63181 ttttacgcta cgggcgggtg aaacactggg tttagtgggc gagtctggtt ccgggaaaag
    63241 cacgacggga ctggcgctgc tgcgactgat tacctctcag ggcagcatcg tctttgacgg
    63301 tcagccactg caagatttaa atcgccgcca gctgttacct actcgtcatc gtattcaggt
    63361 ggtatttcag gatccaaact cctcgctcaa cccacgactc aacgttttgc agattattga
    63421 ggaaggctta cgggttcacc agccgacgct ttctgccgca caacgcgaac aacaagtgat
    63481 agccgtgatg catgaagtgg gattagatcc tgaaacacgc caccgttatc cggcggagtt
    63541 ctctggtggt cagcgacaac gtattgcgat tgccagggca ttaattctta agccctcact
    63601 gatcatactt gatgaaccga catcatcact cgacaaaacg gtacaggcgc aaatattgac
    63661 gctattgaaa tcattgcaac aaaagcatca actggcctat ttgtttatca gccacgattt
    63721 gcacgttgtc cgcgcgttat gtcatcaggt tatcgtactg cgacaagggg aagtagtgga
    63781 acaaggaccg tgcgcgcgcg tgtttgccgc accgcagcag gagtatacgc gtcagctact
    63841 ggcgttgagc tgacgcttaa aaaggattgt agtttgaaaa gggttcggca attgccacac
    63901 caaaattttt caatcggcat gttgcagcaa actcgtcctg gcgatttaca aacaggcacg
    63961 gctcaccttc acactccagc accgaacatg gaacttcgat gtcgcttaac gcctggctta
    64021 gtttatacat caccgaccat gcattatcac catccgggct aagcaattta agcgccacca
    64081 ggctgttgtc accgcacggt ccattttgcg gaatcggttc aaccttcgaa cctgcgaaac
    64141 cagcagacca gcgaaagttc tgcggtagac gatggacgat tgagagttgt aatgaagtca
    64201 cttattttcc ccggaagcac atttacttca caatttattt acatcaattt taacacatca
    64261 tcaacaatcc gtctttatac agatcgtaaa gattgatgcc tcgcctgcat ggcctctatg
    64321 gctctgattt aagtaatatc tgcgggcgtg gtcacgtttg cggggctata tgtacccgtt
    64381 tctgcgatct aactcaacct ttttaactac aatgatgtga ctttttacat aaattgattt
    64441 tacataaaat aaacatatat cgggggaatg atagatttgt ggttgacatg catcaacaaa
    64501 ggaagccttt tagcttcctc gttgtgcaat agatcaccgt tttttcggtc gactggcgta
    64561 cagacagaag agaatggagc tggttgcgca gaatgcaatt gaccaaatca tcggccatgc
    64621 agagttaaag gtcgcaagag aaagcaatgc gccaacaatt gcccctatgc caaaacggaa
    64681 ggttcctgcc agcgaagatg ccgttcccgc catatgggga aactcatcaa gaatgaccgc
    64741 catcgcattg gatgacacca tagacacgca gcccacaaac gccgcaacgc caaccaccag
    64801 cgaccagaat cccagcccca gcagcgcact gatgaccatc cacgctgcca taataaattg
    64861 tatccacaac cccgagcgga acatatttaa cgcgccaatg cggcggacga agcggctgtt
    64921 aaagatggtc atcacgaaca gaaaaacaat gtttagcgca aagtaataac caaagttttc
    64981 cggtgcaacg tggttgattt caatgtagac aaacggtccg gcgctcagga acgagaacat
    65041 tccggcaaag ctgaaaccgc tggccagcat ataactcagg acacgcttat gacggaacag
    65101 cgccgcaaag ttaccaatag tggtacgaat gtgaaaaggc tggcgaggat ccagaagatg
    65161 taatgccagc tcagccacac cagcacccag ccgccaacta tcggtgccat cagcggtgca
    65221 atggtcgtca ccagcatgac aaacgacatc atccgcgaga actcttcttt cgggtaaata
    65281 tcacgcatca aagcgttgat aaccacgctg gccgcagccg cagccagccc gtggaagaaa
    65341 cgcatcacaa tcagctgatc gatggtttgt gccaacgcac acgccaccgc ggcggcggca
    65401 aacaccagcg taccgccaag aaccaccggc ttacgcccga agctgtctgc catcggcccg
    65461 tagattaact gccccaacgc aaagcccaga atataagtac tcagggtcat ctgcgtactg
    65521 cccgccggta cgccaaactg cgctgaaatt accggtagcg cgggcagata catatcaatc
    65581 gacagcggca tcaacatggc cagcaggcca aggataaaaa caatagcaaa cgacgaatgc
    65641 tgtcgggtgg tcacaacggg ctcctgaaag tcattgaaaa gttagacgac gctggcaatt
    65701 tcttcttcag ttaacggacg atattcaccg ggggctaaat cagcatccag cgtaatgccg
    65761 ccaatacgtt cacgatgcag ctcaaccaca tggttaccca cggcggcgaa catgcgtttc
    65821 acctgatgat aacgcccttc gctgatggtc agacgaacct gcgttggggt aatcacttcc
    65881 agcaccgccg gcttagtgag atctttttcg ttatgcagct gcacgccttt agcaaattgt
    65941 tctgccgtat cgtcagctac gggtgatacc agcgtcacca gataggtctt ctcgcaatga
    66001 tggcgcggag aagtaatgcg gtgcgaccac tgaccatcat ctgtcattag taccagaccg
    66061 gtggtatcga tatccagccg ccctgcggcg tgcagtttcc acgctaccgg ttcatctaga
    66121 aaatagagca ccgttgggtg atcagggtcg tccgtggagc aaacataacc ctgaggctta
    66181 ttgagcatga agtaacgtgg accgtgttgc tgcgccagcg ggttgccatc gtaagcgaca
    66241 tcatgttcag gaagcagttt gaacgctgca ttacggacga tttcgccatc gacggtgaca
    66301 cgattgccgc ggatttcacg cccggcaata gcacggctaa cgccgagttg ctgtgcgata
    66361 aatttatcaa gtcgcatgtg tgtgattttg cctgtaaaaa acggaggtcg ggtaatgacc
    66421 cgaatatctg aactgttgtc tgcccagtat agcggtctaa taacatccct caagggaaaa
    66481 agatccgtgg catactatta gcagaataat ctacctacgc gagaccatga tttttacact
    66541 tcgcccatat cagcaagaag ccgtggatgc cacgctcaac cattttcgtc gtcataaaac
    66601 ccctgccgtt atcgtgctgc ccaccggtgc aggtaaaagc ctggtgatag cggaactggc
    66661 acggctggct cgtggtcgcg tactggtgct ggcacacgtt aaagaactgg tggcgcaaaa
    66721 ccatgcaaag tatcaggcgc tggggctgga agccgatatt tttgccgccg ggctgaagcg
    66781 taaagagagc cacggtaaag tggtatttgg cagtgtgcag tcggtcgccc gtaatattga
    66841 tgcctttcag ggtgaatttt cgctgttgat tgtcgatgaa tgtcaccgta ttggtgacga
    66901 tgaagagagc cagtatcagc aaatcctcac tcacctgaca aaagtgaatc cccacttacg
    66961 cctgctgggg ctgactgcca cgccttttcg attgggcaaa ggttggatct accagttcca
    67021 ttatcacggc atggtacgca gcgatgagaa agcccttttc cgtgactgca tttatgagct
    67081 gcccctgcgt tatatgatta aacacagcta tctgacgccg ccagaacgac tggatatgcc
    67141 agtagtgcaa tatgatttca gccgcttgca ggcacagagc aacgggctgt tcagcgaagc
    67201 cgatctcaac cgtgagctga aaaaacagca acgtattacc ccgcacatca tcagccagat
    67261 tatggagttc gccgaaaagc gtaaaggagt gatgattttc gccgcgacgg tcgaacacgc
    67321 aaaagagatt gtgggattac tgcctgccga agatgcagca ctgattactg gcgacacccc
    67381 cggcgctgag cgcgatgtgt taattgaaaa ttttaaagcc cagcgttttc gctatctggt
    67441 caacgtcgcg gtactgacca ccggatttga cgccccgcac gtcgatctta tcgccattct
    67501 gcgccctacc gaatcagtga gtctttacca acaaattgtc gggcgcggtc tgcgtctcgc
    67561 tccgggcaag actgattgtt taattcttga ttatgcgggt aatcctcacg atctctacgc
    67621 gccggaagtt ggtacaccga aaggcaaaag tgacaacgtt ccggtacagg ttttctgccc
    67681 tgcctgcggt tttgccaaca ccttttgggg gaaaacgacc gccgacggga cattgattga
    67741 acactttggt cgccgctgtc agggatggtt tgaagatgac gacggtcatc gcgaacagtg
    67801 tgacttccgt ttccgtttta aaaattgccc gcaatgtaac gcagaaaatg atattgccgc
    67861 tcgccgctgc cgcgaatgtg acacggtact ggttgatccg gatgatatgt taaaagcggc
    67921 gctacgactg aaagatgcgc tggtattacg ctgtagcggc atgtctttgc agcatgggca
    67981 cgacgagaaa ggcgaatggt tgaaaatcac ctattacgat gaagacggtg cggatgtgag
    68041 tgagcgtttc cgtctgcaaa cacctgccca gcgtaccgcc ttcgagcagc tttttatccg
    68101 cccgcatacg cgcacaccgg gcatcccgct gcgctggatc accgccgccg atatcctcgc
    68161 ccagcaagcc ttattgcgac ccccggattt tgtcgtcgcc cgcatgaaag gccagtactg
    68221 gcaggtgcgt gaaaaagtgt tcgattacga aggtcgtttt cgtcgggcgc acgaattacg
    68281 cggttaatgg tagttttgat tgatgtataa ggcgattgag tatagaatct cacccgcttt
    68341 tgcatacgca aagcagatca cttacctgtt gctgggtcgc ctgtagcagg attaatttaa
    68401 gagagaaaga aatgtttact atcaacgcag aagtacgtaa agagcagggt aagggtgcga
    68461 gccgccgcct gcgtgccgct aacaagttcc cggcaatcat ctacggtggc aaagaagcgc
    68521 cgctggctat cgagctggat cacgacaaag tcatgaacat gcaagctaaa gctgaattct
    68581 acagcgaagt tctgaccatc gttgttgacg gtaaagaaat caaagttaaa gctcaggacg
    68641 tacagcgtca cccgtacaaa ccgaagctgc agcacatcga cttcgttcgc gcttaattgc
    68701 tgaataagtt gtaaaaaacc ccgctcccgc ggggtttttt gtatctgcag attatgcctg
    68761 attacggtat tgctattttt tgcaggccag ataaggcgtt cacgccgcat ctggcattca
    68821 gaacacaaaa ccgattaatt gccgccagac gtccggcgct gcaattggtc gcgcaaattc
    68881 ggcggcgtgc ctttaatggt cagggtatcg gtcgccggct cccagaagat gcgttcacct
    68941 aacagcatcg catcaaagtt aatagtcaag ccaccgccgc taccggcaaa cttcgtcaac
    69001 tgacgcagtg tgctccggtc tgccgggaag ctctcttcca gttcgtagcc tttttcagcc
    69061 gcaaactccg tgaagctcac ttcgcttacg cctgccagct ctttcgacag cgattccagt
    69121 tcgatctctt cgcctgcctg cagctgctca ttacagtagc tataaacctg ctggcgcacg
    69181 ttctgtcgct ctgctttatc cagttgtgct tcggcagtga aatcatcaac cgcttgcaac
    69241 agcccacggt tttgtgcttt cgtgttcaat ccttcgctgg ccccgaggaa atccataaag
    69301 aaatccgcca ctttgcgccc tactcgccct ttcaggaaag tgagatagcg ggtggactct
    69361 ggattggttt cccattcggt taaatcgata cgcgcaacaa tatccgcatg attgatatca
    69421 agataatggg ttgggttgat gtcgagattt tcgttaacac gcatactgct caggttgctc
    69481 agaactgcca ccagcagata ctccaccgcc agataacggt agtggcaaaa cagcacgaat
    69541 ccaccgtcag caaaaggata tttcgctaat tcgtcacgca ggcgaccagt tgccgcgcgg
    69601 ctaaatgcca gaaagtcctc ttcgccctga cgctgtaagc gcagcgtctg cgccagttca
    69661 ctctcttcgc taaacaagcc gtaagcttta tttttggcgc tatagacccg atgcagttct
    69721 gccaccatct caacgacggt ttctgtcggt tccagcaatg aatcgcgcaa caccagctca
    69781 aggttctgct catcacgctt gataagctgg tgcagagcaa tctggttgat atccagactc
    69841 atgataaact ctcctttaag accgggcggt attcaaccac cgccggttaa gcgacgcaat
    69901 aaaagatggc ggtcatttta cagaaggcag cgtaacgaga atgatttaac gcataaaaaa
    69961 gcagaaaaaa aaccgttgct acggtaatat gttgcccttt catcaactaa ctgatttcga
    70021 tttatgccac aaatttcccg ctacagtgat gaacaggttg aacaattgct cgcagagctg
    70081 ctcaacgtac tggaaaaaca taaggctccg accgaccttt ccctgatggt gttaggcaac
    70141 atggtcacta accttatcaa caccagcatt gccccggccc aacgccaggc aattgccaac
    70201 tcttttgccc gcgccttaca gtcctctatc aacgaagaca aagcgcacta agggaaacag
    70261 ataacaggtt atggtaactc atcgtcagcg ctaccgtgaa aaagtctccc agatggtcag
    70321 ttgggggcac tggtttgcac tgttcaatat tctgctttcg ctcgtcattg gcagccgtta
    70381 cctgtttatc gccgactggc cgacaacgct tgctggtcgc atttattcct acgtaagcat
    70441 tatcggccat ttcagcttcc tggtgttcgc cacctacttg ctgatcctct tcccgctgac
    70501 ctttatcgtc ggctcccaga ggctgatgag gtttttgtcc gtcattctgg caacggcggg
    70561 aatgacgcta ttactgatcg atagcgaagt ctttactcgt ttccatctcc atcttaatcc
    70621 catcgtctgg cagttggtta tcaacccaga cgaaaatgag atggcgcgcg actggcagct
    70681 gatgttcatc agcgtgccgg ttattttatt gcttgaactg gtgtttgcga cgtggagctg
    70741 gcaaaagctg cgcagcctga cgcgtcgtcg acgcttcgcg cgcccgctgg ccgcattctt
    70801 atttatcgcc tttatcgcct cgcatgtggt gtatatctgg gccgatgcca acttctatcg
    70861 cccgatcacc atgcagcgcg ctaacctgcc gctttcgtac ccgatgacgg cgcgacgttt
    70921 tcttgagaag catggtctgc ttgatgcgca ggagtatcaa cgccgtctta ttgagcaagg
    70981 taatccagac gccgtttccg ttcagtatcc gttaagcgaa ctgcgctatc gcgatatggg
    71041 caccgggcag aatgtgctgt tgattactgt cgatggcctg aactactcac gcttcgagaa
    71101 gcagatgcct gcgctggcag gttttgctga gcaaaatatt tcgttcacgc gccatatgag
    71161 ctccggcaac actacagaca acggcatctt tggcctgttc tatggtatct cgccgagcta
    71221 tatggacggc attctgtcga cccgtacgcc tgcggcatta attactgcgc ttaatcagca
    71281 aggctatcag ctggggttat tctcatcaga tggctttacc agcccgctgt atcgccaggc
    71341 attgttgtca gatttctcga tgccgagcgt acgcacccaa tccgacgagc agaccgccac
    71401 gcagtggatc aactggctgg gccactacgc acaagaagat aaccgctggt tctcgtgggt
    71461 ttctttcaat ggcactaaca ttgacgacag caatcagcag gcatttgcac ggaaatatag
    71521 ccgggcggca ggcaatgtcg atgaccagat caaccgcgtg ctcaatgcac tgcgtgattc
    71581 tggcaaactg gacaatacgg ttgtgattat cactgccggt cggggtattc cgctgagcga
    71641 agacgaagaa acctttgact ggtcccacgg tcatctgcag gtgccattag tgattcactg
    71701 gccaggcacg ccggcgcagc gtattaatgc gctgactgat cataccgatc tgatgacgac
    71761 gctgatgcaa cgcctgctac atgtcagcac acctgccagc gaatattcgc aaggtcagga
    71821 tttgttcaac cctcaacgcc gtcattactg ggttactgca gcggataacg atacgctggc
    71881 aattaccacc ccgaaaaaga cgctggtgct gaacaataac ggtaaatacc gcacttacaa
    71941 cttacgtggt gaaagagtga aagatgaaaa accacagtta agtttgttat tgcaagtgct
    72001 gacagacgag aagcgtttta tcgctaactg attaattata aatcagttag cgaaatatct
    72061 tacttgcaat cggtgtggaa aacggtagta ttagcagcca cgagtcggca cgtagcgcag
    72121 cctggtagcg caccgtcatg gggtgtcggg ggtcggaggt tcaaatcctc tcgtgccgac
    72181 caaaaatccc aagaaaaaac caacctttac ggttggtttt tttatatctg caattaattt
    72241 gataaacaga ccgtgacaca tcacagcctg tttattttct gttatcagaa cgtccagacc
    72301 actcccgcct gagtattcca cggcgattct acccctgcgc cattgccata gctgactgac
    72361 aaatgaccgc ttaacgtcgg ggtaaacgat gaccttatcc cagcctgata aacgccacgg
    72421 gttccagcca catcattatt aaatttgcca tcgtcattaa ctttcacatg gttagaatcg
    72481 gcgtattcct gacgcacggc ggctttcagc cagggttcca acgtcgtacc gttttgcagg
    72541 tccatgtgat agcttaccgc cgttcccgct tcagcgcgta atatccgggt atttcccaca
    72601 tccgcgcgca tgccgtttga taacgtgtag tcctgaccat ctgtggtaaa gccggtaaag
    72661 gcgagatagg gtctaacact ccacaatccg tcaacccaac ggaacccgct ctcaacatga
    72721 gcacccgcgc cgttactatt gtaatcgcca aacgctgttg ccccattact catcttgcca
    72781 tggatggtgt tggcaaaacg gtcaactttc accaccccat caacataggc accgttctga
    72841 tgctcccaac cggcataagc ccccagggtg tagctatcaa tattgccttt gccgccgcga
    72901 tcaaaaccaa tatcagaatg agagtaatca aagaacaagc cgcgaattgt gctgctttct
    72961 tcacgggaga aacggctatc gataccgagc gtcaggcccg tcaatgtttg ctcaaagcca
    73021 gctcccgcat cagtggtcac gttgttgcgg gtgttaattg ccgaactcca catcgccgta
    73081 tcgtaattaa cgccttttac gctaccaaga cgctcacgca cggtgtccag ttctgcatca
    73141 aataccagcg gttgtgctgc tgccatgttc agcacatcag tggttgaagg ggtaatttgc
    73201 gcgcgattct ctgcgaggct ccagctatgg ttgccattat ccagcaaggt atattcatac
    73261 gtaccgatat caacaacgcc tccggcattg cccaacgtga atgcagcatc accgccgccc
    73321 gtcgttacca gtgtaaggct atctcctgct gccgggctgg cccccgtgtc cgtcacgaat
    73381 attttgaaat cacctgttgc ctgaccgatg acgttgagct gatcgctctg atgattagcc
    73441 atatcggtac gcatataaaa ttgccgtttc ccgacagggt attggtggtc aacgtaatga
    73501 aattttccgc acttgccgtc acagatgaac gagtcactgg ctcagccatc ataatcacat
    73561 ttgcgccgga catcgcaagg ttggctaagt tgaggcgagt gttttggttc gtcgtagtga
    73621 tatcggcaat gatggtccat gcgctatttt cgagcgaaac gtccgcttgg ctggtatcca
    73681 cggcaccatg cgttctaaaa gaagcgccct cagaaaccac taccttaccc accaacatag
    73741 cgggtgcata atttattttc ggcttcatga cctcaagaat gccatcattc cctctgactg
    73801 acacgttaac cattgtgcca gcccagacat tagcgcggcc attgttgata accatatttt
    73861 cagagatggc tttagaggaa tacgtgatac ttccattctg atctgatcga ccgagatcat
    73921 atacagcatt tgcctgcact ttcgtaccag tatcctttcc cagcgattgc atagtggcat
    73981 gctcatcaag gatagtgtca atggcctgag tgtcctccat aacaatgagc ccggagccat
    74041 catccagttc ataatttttt gacacaccat cctttatact aaattggcct ttactgtttg
    74101 taccactcac ttccagcgca ttcgttgaga caattaattt cccgcctgct tttttatcaa
    74161 cgccagttgc agtcccgcca tccataacct ccagtaatcc tcccttttcg atagcggcaa
    74221 agacggcaat cccatctttt tcaacaatta agcgcccgcc tttaatcgtg ggatcattcg
    74281 ccgc
//
LOCUS       DAJVQG010000004        64130 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJVQG010000004 DAJVQG010000000
VERSION     DAJVQG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64130
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.004"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(36..1334)
                     /gene="eno"
                     /locus_tag="OQM83_000235"
     CDS             complement(36..1334)
                     /gene="eno"
                     /locus_tag="OQM83_000235"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCS1518598.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAADKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="OQM83_000236"
     CDS             complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="OQM83_000236"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCS1518599.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="OQM83_000237"
     CDS             complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="OQM83_000237"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCS1518600.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="OQM83_000238"
     CDS             complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="OQM83_000238"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HCS1518601.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="OQM83_000239"
     CDS             complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="OQM83_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCS1518602.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4810..7044)
                     /locus_tag="OQM83_000240"
     CDS             complement(4810..7044)
                     /locus_tag="OQM83_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCS1518603.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="OQM83_000241"
     CDS             complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="OQM83_000241"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCS1518604.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLCLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8450..11206
                     /gene="barA"
                     /locus_tag="OQM83_000242"
     CDS             8450..11206
                     /gene="barA"
                     /locus_tag="OQM83_000242"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCS1518605.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11438..12778)
                     /gene="gudD"
                     /locus_tag="OQM83_000243"
     CDS             complement(11438..12778)
                     /gene="gudD"
                     /locus_tag="OQM83_000243"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCS1518606.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKTGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12799..14139)
                     /locus_tag="OQM83_000244"
     CDS             complement(12799..14139)
                     /locus_tag="OQM83_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCS1518607.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14141..15493)
                     /gene="gudP"
                     /locus_tag="OQM83_000245"
     CDS             complement(14141..15493)
                     /gene="gudP"
                     /locus_tag="OQM83_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCS1518608.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSHIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15928..16377)
                     /locus_tag="OQM83_000246"
     CDS             complement(15928..16377)
                     /locus_tag="OQM83_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCS1518609.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16395..17177)
                     /gene="truC"
                     /locus_tag="OQM83_000247"
     CDS             complement(16395..17177)
                     /gene="truC"
                     /locus_tag="OQM83_000247"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCS1518610.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17177..17506)
                     /locus_tag="OQM83_000248"
     CDS             complement(17177..17506)
                     /locus_tag="OQM83_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCS1518611.1"
                     /translation="MTTHDRVRLQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18128..18673)
                     /gene="syd"
                     /locus_tag="OQM83_000249"
     CDS             complement(18128..18673)
                     /gene="syd"
                     /locus_tag="OQM83_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCS1518612.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18741..19589
                     /gene="queF"
                     /locus_tag="OQM83_000250"
     CDS             18741..19589
                     /gene="queF"
                     /locus_tag="OQM83_000250"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCS1518613.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19701..21065
                     /gene="ppnN"
                     /locus_tag="OQM83_000251"
                     /gene_synonym="ygdH"
     CDS             19701..21065
                     /gene="ppnN"
                     /locus_tag="OQM83_000251"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCS1518614.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21621..22910
                     /gene="sdaC"
                     /locus_tag="OQM83_000252"
     CDS             21621..22910
                     /gene="sdaC"
                     /locus_tag="OQM83_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCS1518615.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22968..24335
                     /gene="sdaB"
                     /locus_tag="OQM83_000253"
     CDS             22968..24335
                     /gene="sdaB"
                     /locus_tag="OQM83_000253"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCS1518616.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24447..25202
                     /gene="xni"
                     /locus_tag="OQM83_000254"
     CDS             24447..25202
                     /gene="xni"
                     /locus_tag="OQM83_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCS1518617.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25307..26455)
                     /gene="fucO"
                     /locus_tag="OQM83_000255"
     CDS             complement(25307..26455)
                     /gene="fucO"
                     /locus_tag="OQM83_000255"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCS1518618.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMHYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(26483..27130)
                     /gene="fucA"
                     /locus_tag="OQM83_000256"
     CDS             complement(26483..27130)
                     /gene="fucA"
                     /locus_tag="OQM83_000256"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCS1518619.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVATLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            27677..28993
                     /gene="fucP"
                     /locus_tag="OQM83_000257"
     CDS             27677..28993
                     /gene="fucP"
                     /locus_tag="OQM83_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCS1518620.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQRLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            29026..30801
                     /gene="fucI"
                     /locus_tag="OQM83_000258"
                     /pseudo
     CDS             29026..30801
                     /gene="fucI"
                     /locus_tag="OQM83_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            30910..32328
                     /gene="fucK"
                     /locus_tag="OQM83_000259"
     CDS             30910..32328
                     /gene="fucK"
                     /locus_tag="OQM83_000259"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HCS1518621.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            32330..32752
                     /gene="fucU"
                     /locus_tag="OQM83_000260"
     CDS             32330..32752
                     /gene="fucU"
                     /locus_tag="OQM83_000260"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCS1518622.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            32810..33541
                     /gene="fucR"
                     /locus_tag="OQM83_000261"
     CDS             32810..33541
                     /gene="fucR"
                     /locus_tag="OQM83_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCS1518623.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(33585..34685)
                     /gene="rlmM"
                     /locus_tag="OQM83_000262"
     CDS             complement(33585..34685)
                     /gene="rlmM"
                     /locus_tag="OQM83_000262"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCS1518624.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(34678..35073)
                     /locus_tag="OQM83_000263"
     CDS             complement(34678..35073)
                     /locus_tag="OQM83_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCS1518625.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(35092..36009)
                     /gene="gcvA"
                     /locus_tag="OQM83_000264"
     CDS             complement(35092..36009)
                     /gene="gcvA"
                     /locus_tag="OQM83_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCS1518626.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(36360..36587)
                     /locus_tag="OQM83_000265"
     CDS             complement(36360..36587)
                     /locus_tag="OQM83_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCS1518627.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            36779..37984
                     /gene="csdA"
                     /locus_tag="OQM83_000266"
     CDS             36779..37984
                     /gene="csdA"
                     /locus_tag="OQM83_000266"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCS1518628.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            37984..38427
                     /gene="csdE"
                     /locus_tag="OQM83_000267"
     CDS             37984..38427
                     /gene="csdE"
                     /locus_tag="OQM83_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCS1518629.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(38478..39284)
                     /gene="tcdA"
                     /locus_tag="OQM83_000268"
     CDS             complement(38478..39284)
                     /gene="tcdA"
                     /locus_tag="OQM83_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCS1518630.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(39361..40458)
                     /gene="mltA"
                     /locus_tag="OQM83_000269"
     CDS             complement(39361..40458)
                     /gene="mltA"
                     /locus_tag="OQM83_000269"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCS1518631.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            40667..40743
                     /locus_tag="OQM83_000270"
     tRNA            40667..40743
                     /locus_tag="OQM83_000270"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40701..40703,aa:Met,seq:cat)
     gene            40777..40853
                     /locus_tag="OQM83_000271"
     tRNA            40777..40853
                     /locus_tag="OQM83_000271"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40811..40813,aa:Met,seq:cat)
     gene            40887..40963
                     /locus_tag="OQM83_000272"
     tRNA            40887..40963
                     /locus_tag="OQM83_000272"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40921..40923,aa:Met,seq:cat)
     gene            complement(41038..42291)
                     /gene="amiC"
                     /locus_tag="OQM83_000273"
     CDS             complement(41038..42291)
                     /gene="amiC"
                     /locus_tag="OQM83_000273"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCS1518632.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            42523..43854
                     /gene="argA"
                     /locus_tag="OQM83_000274"
     CDS             42523..43854
                     /gene="argA"
                     /locus_tag="OQM83_000274"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCS1518633.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(43916..45742)
                     /gene="recD"
                     /locus_tag="OQM83_000275"
     CDS             complement(43916..45742)
                     /gene="recD"
                     /locus_tag="OQM83_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCS1518634.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEESLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKRRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(45742..49284)
                     /gene="recB"
                     /locus_tag="OQM83_000276"
     CDS             complement(45742..49284)
                     /gene="recB"
                     /locus_tag="OQM83_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCS1518635.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EVDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(49277..52165)
                     /gene="ptrA"
                     /locus_tag="OQM83_000277"
     CDS             complement(49277..52165)
                     /gene="ptrA"
                     /locus_tag="OQM83_000277"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCS1518636.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(52341..55709)
                     /gene="recC"
                     /locus_tag="OQM83_000278"
     CDS             complement(52341..55709)
                     /gene="recC"
                     /locus_tag="OQM83_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCS1518637.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(55722..56045)
                     /locus_tag="OQM83_000279"
     CDS             complement(55722..56045)
                     /locus_tag="OQM83_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCS1518638.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(56030..56437)
                     /locus_tag="OQM83_000280"
     CDS             complement(56030..56437)
                     /locus_tag="OQM83_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCS1518639.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(56434..56997)
                     /locus_tag="OQM83_000281"
     CDS             complement(56434..56997)
                     /locus_tag="OQM83_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCS1518640.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(56988..57458)
                     /locus_tag="OQM83_000282"
     CDS             complement(56988..57458)
                     /locus_tag="OQM83_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCS1518641.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(57643..58437)
                     /gene="thyA"
                     /locus_tag="OQM83_000283"
     CDS             complement(57643..58437)
                     /gene="thyA"
                     /locus_tag="OQM83_000283"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCS1518642.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(58444..59319)
                     /gene="lgt"
                     /locus_tag="OQM83_000284"
     CDS             complement(58444..59319)
                     /gene="lgt"
                     /locus_tag="OQM83_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCS1518643.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(59470..61716)
                     /gene="ptsP"
                     /locus_tag="OQM83_000285"
     CDS             complement(59470..61716)
                     /gene="ptsP"
                     /locus_tag="OQM83_000285"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1518644.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(61729..62259)
                     /gene="rppH"
                     /locus_tag="OQM83_000286"
     CDS             complement(61729..62259)
                     /gene="rppH"
                     /locus_tag="OQM83_000286"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCS1518645.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            62944..63633
                     /gene="mutH"
                     /locus_tag="OQM83_000287"
     CDS             62944..63633
                     /gene="mutH"
                     /locus_tag="OQM83_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCS1518646.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            63702..>64130
                     /locus_tag="OQM83_000288"
     CDS             63702..>64130
                     /locus_tag="OQM83_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCS1518647.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDS"
BASE COUNT        15541 a        17275 c        15723 g        15591 t
ORIGIN      
        1 ggaggctggc atttttaaat cagataaagt cagtcttatg cttggccttt gatctcttta
       61 cgaccgttgt acggtgcttt ttcgcccaga gcttcttcga tacgaatcag ctggttgtat
      121 ttagcaacac ggtcagaacg gctcatagaa ccagttttga tctggcctgc agcagtacca
      181 acagccaggt cagcgatggt agcgtcttca gtttcgccag aacggtgaga gataactgca
      241 gtgtagccag catctttcgc catcttgatt gcagccagag tttcggtcag agaaccgatc
      301 tggttgaatt tgatcaggat ggagttagcg ataccttttt cgataccttc tttcaggatc
      361 ttggtgttgg ttacgaacag gtcgtcacca accagctgga ttttgtcgcc cagaactttg
      421 gtctggtatg cgaaaccgtc ccagtcagat tcgtccagac cgtcttcgat agatacgatc
      481 ggatactgtt tggtcagttc ttccaggaag tgagtgaatt cttcagaggt gaaagctttg
      541 ttgccttcgc cagccagaac gtatttaccg tctttgtaga attcagaagc tgcgcagtcc
      601 atcgccagag tgatgtcttt gcccagttca taaccagcag ctttaacagc ttcagcgata
      661 acagccagag cttcagcgtt ggaacccagg ttcggcgcat agccaccttc gtcaccaact
      721 gcagtgttca tgcctttcgc tttcagaact tttgccaggt gatggaaaac ttcagaaccc
      781 atgcggatgg cttctttcac agttttcgcg ccaaccggct gaatcatgaa ttcctggata
      841 tcaacgttgt tgtcagcgtg ctcaccaccg ttgatgatgt tcatcatcgg aaccggcatg
      901 gagtatttgc ccggagtgcc gttcagttca gcgatgtgct cgtacagcgg catcccttta
      961 tcggcagcag cagctttggc gttagccaga gatacagcca ggattgcgtt cgcgccgaat
     1021 ttggatttgt tttcggtgcc gtccaggtcg atcatgatct tgtcaatgcc agcctgatct
     1081 ttagcgtctt tgccaatcag tgcctgagcg atcgggccgt ttaccgcagc aacagctttg
     1141 gttacgcctt tacccaggaa acgggatttg tcgccatcgc gcagttccag agcttcacgg
     1201 gaaccagtag aagcacctga cggagcagct gccataccga cgaaaccacc ctccagatgt
     1261 acttcggctt caacagtcgg gttaccacgg gagtcgatga tttcacgacc gatgattttt
     1321 acgattttgg acattaggtt ttcctcaagt cactagttaa actgaaactc cagacaaaca
     1381 acgcgtaccc aaggtacgcg ttgccgttct aactttttta cttacttcgc ctgacgtttc
     1441 tggaactcgc tggcagcttt cacaaagcct gcaaacagcg ggtgaccatc acgtggagta
     1501 gaagtaaact ccggatggaa ctggcaagcc acgaaccacg ggtgattcgg aacttcgatg
     1561 atctcgacca actgatcatc cccggaacgg cccgcaacgc gcagacctgc atcttcaatc
     1621 tgtttcaaca gcatgttgtt gacttcgtaa cggtgacgat gacgctcaac aattgtcggc
     1681 gcattgtaca gctggcgaac caggctatcg tcaaccaact ggcactgctg tgcgccgaga
     1741 cgcatggtac cgccgagatc gctcttctcg ctacgaactt caacgttgcc gttttcatcg
     1801 cgccactcgg taatcagcgc cacaaccggg tacttacagt ctggcacaaa ttccgtagag
     1861 ttggcgttct ccatgttggc aacatggcga gcgtaatcaa ttaacgccac ctgcataccc
     1921 aggcaaatgc ccagataagg aatattgttc tcacgcgcaa aacgcgcggt cgtaatcatg
     1981 ccttctacgc cacgatagcc gaaaccgcca ggtacgagga ttgcgtccag acctttaagg
     2041 atttcaacgc cgcgcgtttc aacatcttgt gaatcgatca gtttgatgtt gacgctgaca
     2101 cgattcttca gcccaccgtg tttcagtgct tcgatcactg atttataagc atccggcagt
     2161 tcaatgtact tgccgaccat accgatggtg acttcactta ccggattcgc ttcttcgaag
     2221 ataacctgtt cccattcgga cagatttgct tccgggcagt ttaagctgaa tcgtttacaa
     2281 atataatcgt ccagcccctg agatttcaac aggcccggaa ttttatagat ggaatcgacg
     2341 tctttcagag aaataaccgc tttttccgga acattacaga acaatgcaat cttcgcacgt
     2401 tcgttcgccg gaacagcgcg atctgaacga caaatcagga tgtcaggctg gataccgatg
     2461 gagagcagct cttttacaga gtgctgagtc ggtttggttt tgacttcacc agacgctgcc
     2521 atgtacggca ccagcgtcag gtgcataaac agagtgtgct cacggccaat ttcaacagcc
     2581 atctggcgaa tcgcttcgag gaacggcaag gattcgatat cacctactgt accgccgatt
     2641 tctaccagta ctacgtcatg accttcgcca ccttccagca cgcgctcttt gattgcgtta
     2701 gtgatgtgcg gaataacctg cacggttgcg ccgaggtagt caccgcggcg ttctttacgc
     2761 agaacgtcag agtagatacg acccgtggtg aagttgttgc ggcggctcat tttggtacga
     2821 atgaaacgct cgtagtgccc caggtccagg tcggtttcag cgccgtcttc agtaacgaac
     2881 acttccccgt gttggattgg gctcatagta cctggatcga cgttgatgta cggatccagt
     2941 ttcatgatgg tcacattgag gccacgggct tcaagaatgg ctgcgaggga ggctgcggca
     3001 atgcctttac ccagagagga tacgaccccg ccggtcacaa aaatatagtt cgttgtcatg
     3061 ctgaacctga gaagttaggt tgaaaagacg atggaataac caggacggga aagcagtata
     3121 cccgaacatg acctgtgcca caaactttca ttatccctcc tcttcgccag cgcactattg
     3181 aaatcaggag tgagaaaata gcccctttgg ggtaaatgtt tttgacgcaa atcaagcgct
     3241 tgtcatttaa aaaatgacac aaatggcgct tgaccgcgta attcccttag agatcaattt
     3301 cctgccgttt tacctgttgc cagacttctt ccattgtttc gaggtcaaca cctgtcattt
     3361 ccagtccacg cgcggcaaca atacgctcca cttcgcgaaa acgacgctcg aatttttcgt
     3421 tcgctttttg caatgcgatt tctgctttcg tccctaaatg gcgagccaga ttaaccgtgg
     3481 caaacagcag gtcccccatt tcctcctcca gtttagcctg gtcgacaaca gcctgccgcg
     3541 cctcgtacat cacctcgtcg atctcttcgt agactttatc aaccaccggg ccaagcgtcg
     3601 tccagtcgaa gccaacgttg gcgcaacgtt tctggatttt ttgcgcccgc attaaagccg
     3661 gtaaactacg aggaatatcg tccagcgccg aatgctgcgc tttctgcgcg cgctcttcgg
     3721 ttttgatttg ctcccaacgg gcaagcactt cactactgtt ttcggcagaa ctatcagcaa
     3781 aaacatgcgg atggcgacgc tctaatttat cgctaatagc agcgcaaata tcattaaagt
     3841 caaagcgccc ttcttcctga gccatttgcg cgtaaaacac cacctggaat agcagatcgc
     3901 ccagttcacc gcgaagatcg tcaaaatctt cacgggcgat ggcgtccagc acttcgtagg
     3961 tttcttcaag ggtgtaaggc gcaatggtgg caaatgtctg ctctttatcc cacgggcagc
     4021 cgttttccgg atcgcgcagg cgctgcataa tagtgagcaa acggtcgatt tgattcattg
     4081 aattgtcctg aaaattgcgg gtctgtcagg tggaaacctg tgaccagaat agaagtgagt
     4141 tagtaacact acccaatcag tacgttaatt ttggctttaa tgagttgtaa ttcctctggg
     4201 gcaactgttc ctttcttcgt tgctcctctt gcccgccagg cgatactttt tacctgatca
     4261 gctaacgcta cgccatcacg ttcctgaccg gataaaacaa cttcgaacgg atatcctttt
     4321 gattgcgttg tacaaggaac acacagacac atacctgttt tgttgttgta catgaaagga
     4381 ctcaggacaa cagctggacg atgtccagct tgctcgctac cttttgtcgg gtcaaaatca
     4441 acccaaatca gatcgcccat atcgggtacg tatcggctta ccattaccag acttccttat
     4501 ctttcggctc tccccagtcg atattctcgt ggaggttttc cggcgtgatg tcgttgacca
     4561 gttcagcaag cgtaaatacg ggctctttac gcactggctc aataattaat ttgccatcca
     4621 ccaggtcaat cttcacttca tcatcaatat tcagattgag cgcctgcatt aacgtagccg
     4681 ggatccgcac cgccggtgaa tttccccaac gctttacgct actgtggatc ataacccttt
     4741 cctcaaaccg ctatcatatg tagatacagt atatatcaat ctacattgta gatacgagca
     4801 aatttctgcc taactcccgt gcaaccgacg cgcgtcgata acatccggca cctggttgag
     4861 tttacccagc acgcggccca gcacctgcag gttgtaaatc tcaatggtca tgtcgatggt
     4921 tgccagttgc tgttttgtgt cgctacggct ggcaacgcca agcacgttca ccttctcgtt
     4981 ggcgagaatg gtcgtgatat cacgtaacaa cccactacga tcattagcca ccacgcggac
     5041 caccagcgaa tatccggcgg agtagctctc accccatacc gcgtcaacaa tgcgttctgg
     5101 cgcatgagag cgcagttccg ccagttgttc gcaatcggcg cggtgtactg aaataccgcg
     5161 cccctgggta atgaagccga caatctcatc tccaggaatc ggctggcagc agcgcgcgat
     5221 gtggtgcatc aggttgccaa caccttcgac taccacgcga ccgttatctt tactgcggtt
     5281 ttgcggcgtg tagctttttt gctgaagttg cttcagcgcg gcggcgtcct gctcttcggc
     5341 actcggctta ttaaattgcg attgcaggaa gttcaccatc tgattgagac ggatatcccc
     5401 gccaccaatc gccgccagca actcgtcgac atcattaaag ttgtaacgcg gcagcagatg
     5461 tttttctgct tctttcaggc tgatccccag atgttccagc tcgtcgtcaa ggatttgccg
     5521 cccagccaga atgtttttgt cacggtcctg tttacggaac caagcgtgaa ttttcgaacg
     5581 cccacggctg gttgtgacgt aaccgaggtt tgggtttaac cagtcacggc tggggttcgg
     5641 ctgtttctgg gtgataattt caatctggtc gcccatctgc agctggtagg tgaacggcac
     5701 aatgcgcccg ccaatttttg ccccgatgca gcggtgtccg acatcactgt ggatgtggta
     5761 agcgaagtcc agcggcgttg atcccgcagg caaatcaacg acatcacctt tcggcgtaaa
     5821 gacatacacc cggtcgtcaa agacctggct gcgtacttcg tcgagcattt cgccggaatc
     5881 agccatctct tcctgccacg caatcagttt acgcagccag gcaatccggt cttcatgtcc
     5941 cgaacgtgcg ccgccagctg ccgcgccctc tttatatttc cagtgcgcag caacacccaa
     6001 ctctgcatct tcatgcatct gtttggtgcg gatttggatc tcaacggttt tgccacctgg
     6061 ccccagaacc acggtatgaa tagactgata accgtttggt ttcgggttag cgacataatc
     6121 gtcaaactca tccggcaggt ggcgatagtg agtgtgcact atccccagtg cggcatagca
     6181 atcctgtaaa cgctcggcga caatacgtac cgcacgcaca tcaaacagct catcaaaggc
     6241 gaggttcttt ttctgcattt tacgccagat gctgtagatg tgtttcggac gaccatacac
     6301 ttccgcttta acgccttcag ctttcatctc agcgcgcaga tgaccaacga actcttcgat
     6361 gtagtgttcg cggtcgagac gccgttcatg cagcagtttg gcaattcgtt tgtattcggt
     6421 tggatggagg taacggaagc agtaatcttc cagttcccat ttcagttgtc cgattccgag
     6481 acggttagcc agcggtgcgt agatgttggt acactctttt gccgccagta cacgttcatc
     6541 ttccggcgca tcttttactt cgcgcagatg agcaatacgc tccgccagtt tgatgactac
     6601 gcagcgaaaa tcatcgacca tcgccaataa catccggcga acgttatcga cctgttcgga
     6661 ggaaacagaa tcagtgtgcg tcgctttcag ctggcggatc gccgccatat cacgcacgcc
     6721 gtgaataagg ttaacaaccg acttaccgac gctctcacgc agcacatctt cgctgactac
     6781 gttggcatca gccagaggga acagcagcgc cgcccgcagc gtgtcaatgt ccatacttaa
     6841 tgtcgagagg atctccacca tctcaacacc acgccacaat aacagactgg catccggatg
     6901 cccctgcgtc tgttgcagac aatacgccca agtttcggct aagcactcac acgacttctg
     6961 gctggtaata cccagacttg cgatccattt ttccggatca aattcaccag ccttattgat
     7021 atgtgcactt cttaccgcaa ccatcgtcct ctcctttagg gaccagacct gccgaaatcg
     7081 gcaaatcgca actatttaac gcgcgagaaa agtaccatcg attccagatg tcccgtgtgt
     7141 gggaacatat ccagcatcgc cagtcgcgca atggtatatc ctgcttttaa taacgcttcg
     7201 ctatcccgag ccagcgttgc agggttacag gatacataaa ctatacgaat aggttccagt
     7261 tttataattt gctgcataac acctgcggca cctgctcgcg ccgggtccag caacacttta
     7321 tcgaagccgt ttttcgccca cggctgcttt gtgacatctt cttcaagatt ttcgtgataa
     7381 aacgtcacat tctgtaagcc attaagacgc gcattctgct ggcctttttc caccagcgcc
     7441 ggaacgcctt ctacacccac cacactggca gcttgtgtcg ccaatggcag tgtaaagttg
     7501 cccataccgc agaacaaatc cagtacgcga tcttcaggtt gcacatccag ccattccaac
     7561 gcacgcgcta ccattttttg gttcacaccc gcattgacct gaataaaatc gcgcgggcta
     7621 aaagttaagc acaacccatt tgagtcatac cagggcatct caccagagac ggtttcgagt
     7681 atctcactat cgggggcgag atacagatcc aggccttcag aatgcgaaaa gcgttccagt
     7741 ttttcgcgat ctgccgaact tagcggtgcg gtatggcgca aaatcatcag cgtgccgctg
     7801 gttgcctgta ccagttcaac atgaccaaga tggcgcataa cttgtaagct gcccaagcat
     7861 gccatgactt tgggcagcaa tgcttcaagt tggggcacta aaatggggca ttgtttaacg
     7921 tcgacaatgt cactggagcc cgctttgcga aaccccatct gaagttgctg tgttttcggt
     7981 aagtagttca gacttaaacg cgcgcgacgg cgatagcccc agggaacatc ggcgatcact
     8041 tcagagactt cgtgtttcat taatcgggcg agtgccgcac ttttgcttcg ctgctgtaaa
     8101 tccacgctgg cgtgttgttg ctgacagccg ccgcatacgc caaaatgagg acagcgtggc
     8161 gtttcgcgtc ccgggctatc gcttaaccgg cgtacgactt tagcgcgggc atactgtttt
     8221 ttatcttcag taacagtaac ttccgcgttt tcctgcggca ataatccggg gataaatagc
     8281 gttttgccgt tatgtcgcgc cacgccctga ccaaaagagt cgaggtcgtt gactgaaacg
     8341 gttatgatct gacgcgtcgt cgtgcgtcgt tttgcagagt agaattgcgc cattggcgag
     8401 actttctcaa tttaacagtg tgaccttaat tgtcccataa cggaactcca tgaccaacta
     8461 cagcctgcgc gcacgcatga tgattctgat cctggcaccg accgtcctta ttggtttatt
     8521 gctgagtatc tttttcgttg tgcatcgcta taacgacttg cagcgtcaac tggaagatgc
     8581 cggagccagc attattgagc cgcttgcagt ttctactgaa tatggcatga gcctgcaaaa
     8641 tcgcgaatct atcggtcagt taataagcgt actgcatcgt cgccattccg atattgttcg
     8701 cgcgatttcg gtttatgatg aaaataaccg actctttgtc acctccaatt ttcatcttga
     8761 tccctcatca atgcagctcg gcagcaacgt gccgtttcct cgccagctca ctgtcactcg
     8821 tgacggcgat attatgatcc tccgcacgcc gattatttct gagagttact cccccgacga
     8881 atcgcccagt agcgatgcca aaaatagtca aaatatgttg ggatatattg cgctggagct
     8941 ggatcttaaa tcggttcgct tgcagcaata taaagagatc tttatttcca gcgtgatgat
     9001 gctgttttgt atcggtattg cgcttatttt tggctggcgc ttaatgcgcg atgtaaccgg
     9061 tccgattcgc aacatggtga ataccgtcga ccgcatccgt cgcgggcaac tcgacagccg
     9121 ggtggaagga tttatgctcg gcgagctgga tatgctgaaa aacggtatca actcgatggc
     9181 aatgtcgctg gctgcttatc acgaagagat gcagcacaat atcgaccagg cgacgtccga
     9241 tctgcgtgaa acgctggagc agatggaaat tcagaacgtt gagttagatc tggcgaaaaa
     9301 gcgcgcccag gaagcggcgc gtattaaatc cgagtttctg gcaaatatgt cacacgagct
     9361 gcgtacacca ctgaatggtg ttattggctt tacccgcctg acgctgaaaa cagaattaac
     9421 accaacgcag cgcgatcacc tgaatacgat tgaacgttcg gcaaataatt tgctggcaat
     9481 tattaatgat gttctcgact tctcgaaact ggaagcaggt aagctgattc tggaaagtat
     9541 tccattccca ctacgcagca cgctggatga agtcgttact ctgctggcac attcttctca
     9601 cgataaaggg ttggaactga cgctcaatat taaaagcgac gtgcctgata acgtgatcgg
     9661 cgacccactg cgattacagc aaatcatcac taacctggtg gggaatgcaa ttaaattcac
     9721 cgagaatggc aacattgata ttctggtaga aaaacgtgcg ctgagtaata ccaaagtgca
     9781 gattgaagtg cagattcggg ataccggcat tggtattcct gaacgcgatc aatcgcgctt
     9841 attccaggcc ttccgacagg ctgatgccag tatttcccgc cgtcatggtg gcaccggtct
     9901 ggggctggtg attacacaaa aactggttaa tgaaatgggc ggcgatattt cgttccatag
     9961 ccagccgaat cgcggttcaa ctttctggtt ccacattaat ctcgatctga acccgaacat
    10021 tattatcgaa gggccatcca cccagtgcct cgccggtaag cgcctggcct atgtcgaacc
    10081 aaactccgca gcagcgcaat gcacgctgga tattttaagt gaaacgccgc tggaagtggt
    10141 ttatagccca acgttctccg cgctgcctcc cgcgcattac gacatgatgt tgttaggcat
    10201 cgcggtgacc ttccgcgagc cgctaacaat gcaacatgag cgattagcga aagcggtatc
    10261 gatgaccgat ttcctgatgc tggcacttcc ttgccatgca caagtcaatg cagaaaaact
    10321 caagcaagat ggtatcggcg cgtgtctgct gaaaccatta acacctacgc gcctgttgcc
    10381 tgccctgacg gaattttgtc atcacaaaca aaacacgctt ttgcctgtaa ccgatgaaag
    10441 caagctggca atgacagtca tggcagttga tgacaacccc gctaacctga aacttatcgg
    10501 cgcattgctg gaagatatgg tgcaacatgt ggaactttgc gatagcgggc atcaggcggt
    10561 tgaacgggcg aaacagatgc cgttcgattt gatcttaatg gatattcaaa tgcctgacat
    10621 ggatggcatt cgggcctgcg agctcatcca ccagctcccc catcagcaac aaacgccggt
    10681 tatcgcggta acggcgcatg caatggccgg gcaaaaagag aagctgcttg gcgcagggat
    10741 gagcgattat ctggcgaaac cgattgaaga agagcgattg cataatttgt tgttgcgcta
    10801 caagcctggc agcggtattt cctctcgcgt cgtgacgccc gaagtcaacg aaattgtggt
    10861 gaacccgaat gcgaccctcg actggcaact ggcactacgc caggcagcag gaaaaaccga
    10921 tctagcgcgc gatatgctgc aaatgttact cgatttcctg cctgaagttc gcaacaaagt
    10981 tgaggagcag ctggttggag aaaacccgga aggcctggtt gatttgattc ataaactgca
    11041 tggcagttgc ggctatagcg gtgtgccgcg tatgaagaat ctctgccaac tgatcgaaca
    11101 acagctacgt agcggtacta aagaagaaga tttggagccg gagctgctgg aactgttgga
    11161 cgagatggat aatgtcgcgc gcgaagccag caaaattctc gggtaacgga cgtcttgtcg
    11221 agtagcaatc ggctttttat gacgctggtt tcagattggt gacaaagtgc ctgtgtttat
    11281 gccggatgcg gcgttaacgc cttatccggc ctacaaaacc aattaaattc aatgaattaa
    11341 aaaatgatgt agacctgata catagcacaa caggctattt tgcgtttagc atcagtctca
    11401 agccggctcc agatagagcc ggttttggtt ttctgtctta acgcaccatg cacgggcgct
    11461 tgttatcgaa cgtccagcca ggaatcagat actgcattcc catcgcatcg tcacgcgcgc
    11521 caagcccgtg tttctgatac agctcatggg ctttcatcac ttgatccata tcgatttcta
    11581 cacccagccc cgttttttcc ggcacctgaa ccagaccgcc tttgatctca aacggttctt
    11641 tggtcaagcg ctgattgcct tcctgccaaa tccagtgcgt atcaatcgcg gtgattttac
    11701 ccggtgcagc ggcggcaaca tgggtaaaca tcgccaggga aatatcgaag tggttgtttg
    11761 agtgtgaacc ccaggtcagg ccaaattcat ggcacatttg cgccacacgt accgaacctt
    11821 gcatagtcca gaaatgcgga tccgccagcg ggatatcaac ggattgtaac gagagcgtat
    11881 ggcccatctg ccgccagtcg gtggcgatca tattggttgc agtcggcagg ccagtcgcgc
    11941 gacggaactc tgccatcact tcacgcccgg agaaaccttg ctccgcacca tacggatctt
    12001 ctgcataagc cagcgaacct ttcaggtatt taccaatttt aattgcttcg ttcagcgacc
    12061 aggcaccgtt aggatcgagc gtaatacgcg cctgcgggaa gcgttgcgcc agtgccacaa
    12121 tagactcggc ttcttcttcc ccggccagta caccgccttt cagtttgaaa tcgttgaagc
    12181 catatttttc atacgccgct tccgctaggc gcaccaccgc atccggcgtc atcgcttctt
    12241 catgacgcag gcgataccag tcgcatgagt catccggctg gctttgatac ggcagaggtg
    12301 tggctttgcg attaccgacg aagaacagat aaccgagcat ttcgacttcg ctacgctgtt
    12361 gaccatcgcc cagcagcgat gccacgttta cccccagatg ctgccccagc agatccagca
    12421 ttgccgcttc tatcccggta actacatgaa tagtggtacg caaatcaaat gtctgcaaac
    12481 cgcgcccacc agcatcacga tcggcaaaag tattacgcac cagcgtcaga acgtttttgt
    12541 attcacccag cgttttacct accaccagcg gaatcgcatc ttccagcgtt ttacggattt
    12601 tctcgccgcc gggaatttcc cctacgccag tgtgaccgga attatctttg ataatcacaa
    12661 tattacgcgt aaagaacggt gcgtgtgccc cactcagatt catcagcata ctgtcatgac
    12721 ccgccaccgg gatgacctgc atttcagtaa caacagacgt cgtaaattga gaactcatat
    12781 ttatgtcctt tttcagaatc aatgacggcc gaaaacggga cgtttacggt caaaggtcca
    12841 gccggggatc aggtactgca tcggacctgc gtcgttacgc gcaccgccag gcagacgttt
    12901 ataggcctca tgtgcctttt gtacctgttc ccagtccagt tccacgccca gaccgggcgc
    12961 atcaggaacg gcaatttttc cgtttttaat ctccagcgga tttttggtca ggcgacaatc
    13021 gccctcctgc caaatccagt gggtatcgat agcggtagga ttacccggtg ccgccgcgcc
    13081 cacatgggta aacatcgcca gagagatatc gaaatggtta ttagaatggc agccccaggt
    13141 cagcccccag tcgtcgcaaa gctgcgccac acggactgca ccggaaagcg tccagaagtg
    13201 cggatcggca agtggaatat ctaccgcatt gagcatcacc gcatgaccca tttcgcgcca
    13261 gttggtggcg atcatgttag tcgcgacggg caagccggtc gcccgccgaa actccgccat
    13321 aacttcacga ccggagaaac cctgttctgc gccgcatgga tcttcggcat aggtaagaac
    13381 atcattcagc cctttgcata gcgaaatggc ttcatcaagt agccaggcac cgttgggatc
    13441 aacggtaatc cgcgcatcag ggaagcgttt cttcaatgca cgaacagtgt cgatttcttg
    13501 ctcgccaggt aacaccccgc ccttaagttt gaaatcttta aagccatagc gatcctgtga
    13561 ggcttccgcc agacgcacaa cggcttcgct gttcatcgct ttctgatggc gcaactgata
    13621 ccactcatgg ttgcccggcg tattttccag ataaggaaga tcggttttgg tccgatcacc
    13681 gatataaaac agataaccga ggacggtaat agtctcgcgt tgcttgcctg gccccaacag
    13741 ttcgcagacc ggaacattca gcgccttacc tagcaggtca agcaaggcgg cttccagcgc
    13801 cgccacggca ttaacgcgca attcaaaagt ccaggcacct ttgccgaagg tatcaaaatc
    13861 ggctgcctga ttacctttat gcacctgctg aaccacttta ttcaggcgcg caacttcctg
    13921 gcccagaacc atcggaatag catcgaccag tgtttgataa atcacctctc cgcccggcgc
    13981 ttcaccaatg ccggtatgcc cggcgttatc ggtgagtacc acaatattgc gggtgaaata
    14041 tgcgttatgt gccccaccaa tattaagcaa catgctatcg tgcccggcga ccggaatgac
    14101 tttcatatca gtaataacag gactggattg tgtcgtcatc ttattgcccc gcgacaggtt
    14161 tcagctcgat acgcttgata tcgcccacca gcaccaggta gctcagtacc gcgattaagg
    14221 catgaacacc aacataaatc agcgccccat taaaggagcc agtcgtgcca acgatataac
    14281 caattgcgat tggcgtgacg atgccagaaa tgttaccgaa catgttgaac aggccaccgg
    14341 aaagaccgct gatctctttt ggcgcggtat ctgccattac tgcccaaccc agcgccccga
    14401 tgcccttacc gaagaaggcc agcgccataa agccgatgat catccactca acgttgacgt
    14461 agttgcagaa caccatcacc atcgacagca acatgcccat tacgatcggt gttttacgcg
    14521 caatgttcag cgatcccgtg cgacgcatca gccagtcgga aataatccca cccagcacac
    14581 cgccgataaa accgcaaacc gccggaacgg aagccacaaa gcccgctttc agaatcgaca
    14641 tcccacgcgc ctgcaccaga taaaccggaa accaggtaat gaaaaagtaa gtcagggcgt
    14701 tgatacagta ctgaccgata taaacgccga tcatcatccg tgacccgagc agttgtttga
    14761 tctgccccca cttcacgctg aacggcactt taactttggt gttttgctga tccatattga
    14821 tcagcgcgcc acccgcggcg atgtactcca gctctttctg atttaccccc ggatgttgat
    14881 ttggctcgtg gatgactttc aaccagataa agctgatgac aatccccaga ccgcccataa
    14941 agaagaagac gtgtgaccag cccacttcat gcgtcagcca gcccataatc ggcgcgaaga
    15001 tcaccgttgc gaagtattga gcggagttaa aaatcgacac cgccgttccc ctttcctgcg
    15061 ccggaaacca ggccgcaaca atgtgactgt tgccggggaa agatggcgct tcagcaagcc
    15121 cgaccaggaa gcgcagagta aacagggcaa cgataatgcc gaatccacta aagatatcga
    15181 cgaagccttg cagcaaggta aacatcgacc agataaagat cgaccagaag tagacgcgtt
    15241 ttgaaccaaa acgatccagc aaccaaccac cagggatctg cccgataaca taagcccatg
    15301 agaaggcaga gaacacatag cccattccca cgggatcaag gccgatatct ttggccattt
    15361 ccgaaccggc aatagaaagc gtagcgcggt cgccgtagtt gaaggatgtg acgataaaca
    15421 acatcaccac tatccagtaa cgagcattcg tgcgtttttc cacactgctc gcagcctgac
    15481 ttaaagaact cattgttgca ctcctgaaaa ttcgcgttag ccacgctcac tctggactgc
    15541 gacatagcca ggaaatcaga ggtgacgtag ggtgtttttt gccgttttta taggtcgttc
    15601 gccgaatacg gcgcgtgttt atatctggca atagcagtat aaaaagcgcg ccatagcggc
    15661 tcaccgtgca acaacacaac attaatgcgt tcaatgaggc ccgattttgg cattagccct
    15721 ggacggtgga atccacttca cggaaatgaa aacaagaaca agaaaggaag ggttaaaacg
    15781 aagaaataaa gaagagtatg aaatggatcg cttgactcca ggcaaacgcc agtaaaaatc
    15841 cgcgctatga agcagttttt actggcattt gcctgaaaag attcgattca gcaccgctaa
    15901 aacgacattt accgctcgct gaacatatca ggacaacagc gtgccccact gttcgaccca
    15961 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16021 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcagaa
    16081 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16141 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16201 ggtcgtggac gtaaccacca gaacatactt atcctgataa ggcagccagt cgcttaattc
    16261 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16321 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16381 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16441 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16501 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16561 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16621 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatgt
    16681 cggcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16741 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16801 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    16861 tccaccagtg gataatccag caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    16921 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    16981 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17041 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17101 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17161 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17221 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17281 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17341 tcagcaccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17401 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17461 cttcaagcgt ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacttcaat
    17521 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17581 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17641 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17701 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17761 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    17821 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    17881 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    17941 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18001 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18061 gcttatttcc tggcgatctc ctacagggtc tgtaagagat ctctcacaac cagatggggt
    18121 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    18181 gcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    18241 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    18301 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    18361 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    18421 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    18481 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    18541 tagttgaaat aatgcacggt gaaggaacac cgtacagttc ctcacttaac ggccagcttt
    18601 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    18661 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    18721 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    18781 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    18841 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    18901 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    18961 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19021 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19081 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    19141 ttgatgaact ggaaggccag ccgataggtc attttaatgg cacttgcatt gatgaccagg
    19201 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc acctgtggtg
    19261 aaaaagtagt ggaagagacg cttgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    19321 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    19381 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    19441 aacgcatctt taatgacctg ttacgcttct gccagccaga aaaattgagc gtttacgcac
    19501 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    19561 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    19621 gcagaaggtt gtgaaaggac atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    19681 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    19741 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    19801 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    19861 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    19921 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgcgttgc
    19981 aggccaactt gttcgcggtt ctgcgtgaca ttctcttcgt ttacgggcaa atccataaca
    20041 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20101 ccatcttgcg taacgctcgc gcactgcatg tcggtgaagc gccaaatatg gtggtctgct
    20161 ggggcggaca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc gggaatcagc
    20221 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc gggagcgatg gaagcgccga
    20281 tgaaaggtgc tgcggtcgga cacgcacagc agcgttacaa agacagtcgt tttattggta
    20341 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc gctggtcaac gaattgatca
    20401 tcatgccaga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    20461 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    20521 acccggccaa caaagatcag gttttaccat tgttcctcac cggcccgaaa gagagcgccg
    20581 actacttccg cgtactggac gagtttgtcg tgcatacgtt gggtgaaaac gcgcgccgcc
    20641 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    20701 cgctggtgaa agaaaatcgc cgcgacacag gcgatgccta cagctttaac tggtcaatgc
    20761 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    20821 agctttaccc ggatcaacct gttgaagtgc tggctgccga tctgcgccgt gcgttctccg
    20881 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    20941 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    21001 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    21061 cgtaatctcc ctcccccggt gacgtttgtt gccgggtttt ctcgtttttg gtcacatacc
    21121 catcaattta tttcatttgt tatatgaatg tttcttacca ccctcacgcg ataaatatca
    21181 ttgcagttaa tatgtcatac aatttatgtt gtaacgcaaa cgtttcccta ttttcataaa
    21241 ccacatttta tcgctatgaa aaagaaatta tcgccatgat taactaaaag tattgatttt
    21301 ttcagttcaa cctacatata ttgcgcgccc cggaagaagt cagatgtcgt ttaatgggca
    21361 aatattgccc ttaaattctc ttttactttt gatttacaga gtaaagcgtt gggataatct
    21421 atcttccaag tagattattg tatttgggat caagatcact gatagataca taacttgtgt
    21481 gtatctttcc gccctcaaat tattacggcg gtaaatgatt aagccatcgc cgatagacag
    21541 atttcatttt tacggtcagg caccttcccg agctgaactg gctaaaagct gaattatttg
    21601 cattcctcca ggagaaatag atggaaacga ctcaaaccag cacgattgcg tcgaaagact
    21661 ctcgtagtgc ctggcgcaag acagacacca tgtggatgct gggcctttac ggcacggcaa
    21721 tcggcgcggg cgtgctgttc ctgccaatca acgccggtgt tggcggtatg atcccgctga
    21781 tcatcatggc tatccttgcg ttcccgatga cattttttgc tcaccgcggc ctgactcgct
    21841 tcgtactgtc tggtaaaaac ccaggcgaag acatcaccga ggttgtagaa gaacactttg
    21901 gtattggcgc aggtaaactg attaccctgc tctacttctt cgctatctac ccgatcctgc
    21961 tggtttatag cgtggcaatc accaatacag ttgaaagctt catgtctcac cagctgggta
    22021 tgacaccacc gccgcgtgcg attctgtcgc tgatcctgat cgtgggtatg atgaccatcg
    22081 ttcgcttcgg tgagcagatg atcgttaaag caatgagtat tctggtattc ccgtttgttg
    22141 gcgtactgat gctgctggct ctgtacctga tcccgcagtg gaacggcgca gcactggaaa
    22201 cgctgtctct ggacactgca tctgcaaccg gaaacggtct gtggatgacc ctgtggctgg
    22261 caattccggt tatggtgttc tctttcaacc actctccgat catctcttct ttcgccgttg
    22321 cgaagcgtga agagtacggc gatatggcag aacagaaatg ctcgaagatc ctggcattcg
    22381 cacacatcat gatggtgctg accgtaatgt tcttcgtctt cagctgtgta ctgagcctga
    22441 ctccggcaga cctggctgcg gctaaagagc agaacatctc gattctgtct tacctggcta
    22501 accactttaa cgcaccggtt atcgcgtgga tggctccgat tatcgcgatt atcgctatca
    22561 ccaaatcctt cctcggccac tacctgggcg cacgtgaagg cttcaacggt atggtgatta
    22621 aatctctgcg tggtaaaggt aagtctatcg aaatcaacaa gctgaaccgt atcactgcgc
    22681 tgttcatgct ggtaacgacc tggattgttg ccaccctgaa cccgagcatc ctgggtatga
    22741 ttgaaaccct gggcggccca atcatcgcga tgatcctgtt cctgatgccg atgtacgcaa
    22801 ttcagaaagt accggcaatg cgtaagtaca gcggtcacat cagcaacgta ttcgttgtcg
    22861 tgatgggtct gattgcaatc tccgcaatct tctactctct gttcagctaa gtcctttcgc
    22921 gccgctttcg ggcggcgctt cctccgtttt aacacgatgt atttcctatg attagcgtat
    22981 tcgatatttt caaaatcggc attggccctt ccagttctca taccgttgga ccaatgaaag
    23041 cgggtaaaca atttaccgac gatctgattg cccgtaatct gcttaaagac gtgacccgcg
    23101 tggtggttga cgtgtacggc tcgctctctc tgaccggtaa aggccaccac actgatatcg
    23161 ccattattat gggcctggcg ggtaacctgc cggataccgt ggatatcgat tccatccctg
    23221 gttttattca ggatgtgaat actcatggtc gcctgatgct ggcaaacggt cagcatgaag
    23281 tggagttccc ggttgatcag tgcatgaact tccatgcaga caacctttct ctgcatgaaa
    23341 acggtatgcg cattaccgcg ctggcgggcg ataaagtcgt ttacagccag acttactact
    23401 ctattggcgg tggctttatc gttgatgaag agcattttgg ccagcagaat agcgcaccgg
    23461 ttgaagttcc ttatccgtac agttcagcag ccgatctgca aaaacattgt caggaaaccg
    23521 ggctgtcact ctctggcctg atgatgaaaa atgagctggc gctgcacagc aaagaagagc
    23581 tggaacagca cctggcgaac gtatgggaag tcatgcgtgg cggtattgag cgcggtattt
    23641 ccaccgaagg cgtgttgcct ggcaaactgc gcgttccacg ccgtgctgcg gcactacgcc
    23701 ggatgctggt cagccaggat aaaaccacca ctgacccgat ggcggttgtt gactggatca
    23761 acatgtttgc actggcagtg aacgaagaga acgctgctgg cggacgcgtg gtgactgcgc
    23821 cgactaacgg tgcgtgcggg attatcccgg cagtgctggc gtactacgac aagttcatcc
    23881 gcgaagtgaa cgctaactca ctggctcgtt acctgctggt agccagtgcc attggttctc
    23941 tttataagat gaacgcgtca atttctggtg ctgaagtcgg ctgccagggt gaagttggtg
    24001 tggcgtgctc aatggcggcg gctggtctgg cagaactgtt aggtgcaagc ccggcgcagg
    24061 tgtgcatcgc ggcggaaatc gccatggagc acaacctcgg tctgacgtgt gacccggtcg
    24121 ccggacaggt ccaggtgcca tgcatcgagc gtaacgccat tgcggcagta aaagcggtga
    24181 acgccgcacg tatggcgcta cgccgtacca gcgagccgcg cgtctgcctc gataaagtta
    24241 tcgaaaccat gtacgaaaca ggtaaagata tgaacgccaa gtaccgcgaa acctctcgcg
    24301 gcggcctggc aatgaagatc gttgcctgcg attaatcgct ctccagaggc ctcgttttgc
    24361 gaggcctctt cccgatttct catccagccg tagcctgttc cggcatcgaa tgttaccctt
    24421 atcgcctgat ctttaagggg gttatcgtgg ctgttcattt gcttattgtc gatgcactga
    24481 atcttattcg tcgcattcat gccgttcagg gatcgccctg tgtcgaaacc tgccagcatg
    24541 cgctcgatca gctcattatg cacagccagc caacccacgc ggtcgccgtt tttgatgatg
    24601 aaaaccgcag tagcggctgg cgtcatcagc gtttaccaga ttacaaagcg ggtcgaccgc
    24661 caatgccgga agagttgcac gacgagatgc ctgcattacg cgccgccttt gagcaacgcg
    24721 gcgtcccgtg ctggtcaacc agcggcaacg aagccgatga cttagccgcc acgctggcgg
    24781 tcaaagtgac acaggccggg catcaggcaa cgattgtttc gacagataaa ggctactgtc
    24841 agttactttc accgacatta cgtattcgtg attacttcca gaaacgttgg ctggatgcgc
    24901 catttatcga taaagaattt ggcgttcaac cgcagcagtt gcccgattac tggggacttg
    24961 cggggatcag cagttcaaag gtaccgggtg ttgcgggaat cggaccaaaa agcgccacgc
    25021 agctgctggt cgagtttcag agtctggaag ggatatatga gaatctggat gcggttgccg
    25081 aaaagtggcg caaaaaatta gaaacccata aagagatggc gtttctgtgc cgcgatattg
    25141 cccgcttaca aaccgatttg catatcgacg gcaatttaca gcaattgcgg ttggtacggt
    25201 aacggcgagc cggatgcaaa cgattatcaa cacattgaat ttaatgcact tatataccgg
    25261 ataagacgcg gctagcgtcg tatccggcat tatcacatca gcgcatttac caggcggtat
    25321 ggtaaagctc tacaatatcc tcaagcgttg cttcacgcgg gttgccaccg gtacaaacat
    25381 catccagtgc cgcctgcgcc agtgccggaa tgtcttcctt gcgtacacca acatcacgca
    25441 aatgtggcgg aataccgaca tcacggttga gagcaaacac cgcttcaaca gcggcattac
    25501 gcgcctcttc caggctcata ccttccactt tcacgcccat aacgcgcgcg atatcgcggt
    25561 acttctcacc ggtaaagtca gcgttatagt gcatgacatg cggtaacagg atggcgttcg
    25621 caacaccgtg tggagtgttg taaaacgcgc ccagtggatg cgccatacca tgcaccaacc
    25681 ctaacccaac attcgagaag cccatacccg caacatactg cccgagcgcc atttcttctc
    25741 cggcatcctt atcaccagca accgatcctc gcagcgcccc agcaatgatt tcaatcgctt
    25801 taatgtgcag tgcatcggtt agcgcccacg cgccacgggt aatatacccc tcaatagcat
    25861 gagtgagcgc atcgacaccc gtcgcagctt tcagcgctgg aggcatacca tccatcatgt
    25921 cagcgtcaat aaacgccacc tgcgggatat catgcggatc aacgcaaaca aacttgcgcc
    25981 gtttctcttc gtcagtgatc acgtagttaa tggtcacttc tgccgcagta cctgctgtgg
    26041 taggaattgc cagaatcggt acactgggtt tattggtcgg ggaaagccct tccaggctac
    26101 gcacatcggc aaactccgga ttgttgctga taatgccaat cgctttacaa gtatcctgtg
    26161 gagaaccacc accaatagcg atcaggtaat ccgcgccgct attctggaat acaccgagcc
    26221 cttctttgac gacagtaatt gttgggttgg gcactacgcc gtcgtaaatc gcccatgcca
    26281 gccctgcagc atccatctta tcggtcactt tcgccaccac gccgcattgc accagcgttt
    26341 tatcggtgac gatcagcgcc ttctgataac cacggcgttt cacctcatcg gttaaagccc
    26401 caacagcacc ccgaccaaac catgccgttt cgttcagaat cattctgtta gccatcatcc
    26461 ttctccttgt tgctttacga aattactctt caatccgtaa cccataggtt ttgaatttct
    26521 ccagcactac ggcaatctct tcatcgctca gcactggcac cgggtccgta atcgccaggg
    26581 tcgccacgta aagttgcgcc agcacttcaa cttcatgcgc cagccataac gctttttcca
    26641 gattcacctc acaagcgata agcccatgat gttgtaacaa agttgcctta cgatttttga
    26701 gagccagcgc aacatgttca gaaagttcgc gtgttccaaa ggtcgcataa ggcgcgcaag
    26761 gaatagaatt accacctgcc gccgcaatca tgtagtgaat agcggggatc ggtcggttaa
    26821 gaatggaaac tgctgtgcaa tgaacggcat gattgtgaac aaccgcgttg gcatccggtc
    26881 tgctttgata ggccgccata tggaaacgcc attcgcttga ggggagcttt ccttcctcat
    26941 gtttaccgtt gccatcaata aagacaatat gcgactccgt cagtttttca tatggaatgc
    27001 ctgtaggcgt aatcagcatc ccatcctgat aacgtacact gacgttcccc gctgtcccct
    27061 ggttcagtcc caggcgggtc atttccaggc aagtgtcaat aatctgacga gcaagtttat
    27121 ttcgttccat tagctacctc tctctgattc aaaacagggc aataatgttg ttcctttcac
    27181 actattgaat tagccgttta attacccacc atcttcttcc tgattaacaa gaaagaaatt
    27241 cacaagctta tattttgtga cctagctcaa ctaatcacag taaataactg caagttctct
    27301 ttttataacc ccattaaaaa tgaccgctct taaaaatatt tatcaaaacg gtcatttttc
    27361 tattcaccca agccaggaat gcccgttttc ggcataaaca attaatacag tcatctggtt
    27421 tgtgtttttt atgatttatt ttctgaaacg ggcatgaaat ttcgattatt aaagtgatgg
    27481 tagtcacata aagtcacctt ccagctaata agtgtgaccg ccgtcatatt acagagcgtt
    27541 ttttatttga aaatgaatcc atgagttcat ttcagacagg caaatattca ctgatatgaa
    27601 gcccgaactc gctggttttg cacttttgaa aacataaccg attacgtgct taagcttctg
    27661 aacctaagag gatgctatgg gaaacacatc aatacaaacg cagagttacc gtgcggtaga
    27721 taaagatgca gggcaaagca gaagttacat tattccattc gcgctgctgt gctcactgtt
    27781 ttttctttgg gcggtagcca ataaccttaa cgacatttta ttacctcaat tccagcaggc
    27841 ttttacgctg acaaatttcc aggctggcct gatccaatcg gccttttact ttggttattt
    27901 cattatccca attcctgctg ggatattgat gaaaaaactc agttataaag caggaattat
    27961 tactgggtta tttttatatg ccctgggcgc tgcattattc tggcccgctg cagaaataat
    28021 gaactacacc ttatttttag ttggcctatt tattattgca gccggattag gttgtctgga
    28081 aactgccgca aacccttttg ttacggtatt agggccggaa agcagtggtc acttccgctt
    28141 aaatcttgcg caaacattta actcgtttgg cgcaattatc gcggttgtct ttgggcaaag
    28201 acttattttg tctaacgtgc cacatcaatc gcaagacgtt ctcgataaaa tgtctccaga
    28261 gcaattgagt gcgtataaac acagcctggt attatcggta cagacacctt atatgatcat
    28321 cgtggctatc gtgttactgg tcgccctgct gatcatgctg acgaaattcc cggcattgca
    28381 gagtgataat cacagtgacg ccaaacaagg atcgttctcc gcatcgcttt ctcgcctagc
    28441 gcgtattcgc cactggcgct gggcagtatt agcgcaattc tgctacgttg gcgcacaaac
    28501 ggcctgctgg agctatttga ttcgctacgc tgtagaagaa attccaggta tgactgcagg
    28561 ctttgccgct aactatttaa ccggaaccat ggtgtgcttc tttattggtc gtttcaccgg
    28621 tacctggctc atcagtcgct tcgcaccaca caaagtcctg gccgcctacg cattaatcgc
    28681 tatggcactg tgcctgatct cagccttcgc tggcggtcat gtgggcttaa tagccctgac
    28741 tttatgcagc gcctttatgt cgattcagta cccaacaatc ttctcgctgg gcattaagaa
    28801 tctcggccag gacaccaaat acggttcgtc cttcatcgtt atgaccatta ttggcggcgg
    28861 tattgtcact ccggtcatgg gttttgtcag tgacgcggcg ggcaacatcc ccactgctga
    28921 actgatcccc acactctgct tcgcggtcat ctttatcttt gcccgtttcc gctctcaaac
    28981 ggcaactaac tgaacatatt ttccgaataa agtgaggaat ctgtaatgaa aaaaatcagc
    29041 ttaccgaaaa ttggtatccg cccggttatt gacggtcgtc gcatgggtgt tcgtgagtcg
    29101 cttgaagaac aaacaatgaa tatggcgaaa gctacggccg cactgctgac cgagaaactg
    29161 cgccatgcct gcggagctgc cgtcgagtgt gtcatttccg atacctgtat cgcgggtatg
    29221 gctgaagccg ctgcttgcga agaaaaattc agcagctaga atgtaggcct caccattacg
    29281 gtaacgcctt gctggtgcta tggcagtgaa accatcgaca tggatccaac ccgcccgaaa
    29341 gccatttggg gctttaacgg cactgaacgt cccggcgctg tttacctggc agcggctctg
    29401 gcagctcaca gccagaaagg catcccggca ttctccattt acggtcatga cgttcaggat
    29461 gccgatgaca catcgattcc tgccgatgtt gaagaaaaac tgctgcgctt tgcccgcgcc
    29521 ggtttggccg tcgccagcat gaaaggtaaa agctatctgt cgctgggcgg cgtttcgatg
    29581 ggtatcgccg gttctattgt tgatcacaac ttctttgaat cctggctggg aatgaaagtc
    29641 caggcggtgg atatgaccga actgcgtcgc cgtatcgatc agaagattta cgacgaagcc
    29701 gaactggaaa tggcactgac ctgggctgat aaaaacttcc gctatggcga agatgaaaat
    29761 aacaaacagt atcaacgcaa tgccgagcaa agccgcgcag ttctgcgcga aagtttactg
    29821 atggcgatgt gtatccgcga catgatgcaa ggcaacagca aactggccga tattggtcgc
    29881 gtggaagaat cacttggcta caacgccatc gctgcgggct tccaggggca acgtcactgg
    29941 accgatcaat atcccaatgg tgacaccgcc gaagcgatcc tcaacagttc atttgactgg
    30001 aatggcgtgc gcgaaccctt tgtcgtggcg accgaaaacg acagtcttaa cggcgtggca
    30061 atgctaatgg gtcaccagct caccggcacc gctcaggtat ttgccgatgt gcgtacctac
    30121 tggtcaccag aagcaattga gcgtgtaacg gggcataaac tcgatggact ggcagaacac
    30181 ggcatcatcc atttgatcaa ctcaggttct gctgcgctgg acggttcctg taaacaacgc
    30241 gacagcgaag gtaacccgac gatgaagcca cactgggaaa tctctcagca agaggctgac
    30301 gcttgcctcg ccgctaccga atggtgcccg gcgatccacg aatacttccg tggcggcggt
    30361 tactcttccc gcttccttac cgaaggcggc gtcccgttca ccatgactcg tgtcaacatc
    30421 atcaaaggcc tgggatcggt actgcaaatc gcggaaggct ggagcgtgga attgccgaag
    30481 gatgtgcatg acatcctcaa caaacgcacc aactcaacct ggccaaccac ctggtttgca
    30541 ccgcgcctca ccggtaaagg gccgtttacg gatgtgtact cggtaatggc gaactggggc
    30601 gctaaccatg gggttctgac catcggccac gttggcgcag actttatcac tctcgcctcc
    30661 atgctgcgta tcccggtatg tatgcacaac gttgaagaga ccaaagtgta tcgtccttct
    30721 gcctgggctg cgcacggcat ggatattgaa ggccaggatt accgcgcttg ccagaactac
    30781 ggtccgttgt acaagcgtta atacattact ccctgatgtg atgcccggtc gctccggcta
    30841 ccgggcctga acaagcaaga gtggttagcc ggataagcaa tgttatccgg ctatattgca
    30901 ggagcgatta tgaaacaaga agttatcctg gtactcgact gtggcgcgac caatgtcagg
    30961 gccatcgcgg ttaatcggca gggaaaaatt gttgcccgcg cctcaacgcc taatgccagc
    31021 gatatcgcga tggaaaacaa cacctggcac cagtggtctt tagacgccat tttgcaacgc
    31081 tttgctgatt gctgtcggca aatcaatagt gaactgactg aatgccacat ccgcggtatc
    31141 gccgtcacca cctttggtgt ggatggcgct ctggtagata agcaaggcaa tctgctctat
    31201 ccgatcatta gctggaaatg tccgcgaaca gcagcggtta tggacaatat tgaacagttg
    31261 atctccgcac agcggttgca ggctatttct ggcgtcggag cctttagttt caatacgtta
    31321 tataagttgg tgtggttgaa agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg
    31381 ctctttattt cgtcgctgat taaccaccgt ttaaccggcg aattcactac tgatatcacg
    31441 atggccggaa ccagccagat gctggatatc cagcaacgcg atttcagtcc gcaaatttta
    31501 caagccaccg gtattccacg ccgactcttc cctcgtctgg tggaagcggg tgaacagatt
    31561 ggtacgctac agaacagcgc cgcagcaatg ctcggcttac ccgttggcat accggtgatt
    31621 tccgcaggtc acgataccca gttcgccctt tttggcgctg gtgccgacca aaatgaaccc
    31681 gtgctctctt ccggtacatg ggaaatttta atggtccgta gcgcccaggt tgatacttcg
    31741 ctgttaagtc agtacgccga ttctacctgc gaactggata gccaggcagg gttgtataac
    31801 ccaggtatgc aatggctggc atccggcgtg ctggaatggg tgagaaaact gttctggacg
    31861 gctgaaacac cctggcaaat gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat
    31921 ggtgtgaaaa tgcagtgtga tttattgtcg tgtcagaacg ctggctggca aggagtgacg
    31981 cttaatacca cgcgggggca tttctatcgc gcggcgctgg aagggttaac tgcgcaatta
    32041 cagcgcaatc tacagatgct ggaaaaaatc gggcacttta aagcctctga attattgtta
    32101 gtcggtggag gaagtcgcaa cacattgtgg aatcagatta aagccaatat gcttgatatt
    32161 ccggtaaaag ttctcgacga tgccgaaacg accgtcgcag gagctgcgct gttcggttgg
    32221 tatggcgtag gggaatttaa cagcccggaa gaagcccgtg cacagattca ttatcagtac
    32281 cgttatttct acccgcaaac tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac
    32341 aatttcgccg ttaatttctc ccgaactatt gaaagtgctg gcagagatgg gacatggaga
    32401 tgaaattatt ttttccgatg ctcactttcc cgcccattcg atgggaccgc aggtgatccg
    32461 cgctgatggc ctgttggtga gcgacttgct ccaagcgatt atcccattat ttgagctgga
    32521 cagttatgcc ccgccgctgg tgatgatggc agcggtagaa ggtgacactc tcgatcctga
    32581 agtagaacga cgttaccgta atgcgctttc actacaagcc ccgtgtcctg acatcatccg
    32641 catcaatcgt tttgcgtttt atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg
    32701 cgaacgagcg aagtacggga atattctttt aaaaaaaggg gtaacaccgt aatctcatat
    32761 cggtacgccc gcatgacgcg ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc
    32821 acgccagcaa gcgatagtcg acctgctgct gaaccatacc agcctgacca cggaagctct
    32881 ctctgaacag ctaaaggtca gtaaagaaac cattcgtcgc gatctcaatg aattacagac
    32941 gcagggtaaa attctgcgca atcatggacg cgctaaatat atccaccgtc aaaatcaaga
    33001 cagtggcgat ccctttcaca tcaggctgaa aagccattat gcgcataaag cagatatcgc
    33061 gcgcgaggcg ctcgcgtgga ttgaagaagg gatggtgata gccttagacg ccagttcaac
    33121 ttgctggtat ctggcacgcc agttgcctga catcaacatt caggtcttca ccaatagcca
    33181 tccgatttgc catgaactcg gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac
    33241 acttgagcgc aaatatggct gttacgtcaa tccctcgctg atttcccaac ttaaatcgct
    33301 ggaaatcgat ctgtttattt tttcttgtga agggatcgat agcagcggcg cactgtggga
    33361 ctccaatgcg atcaacgctg attacaaatc gatgctatta aaacgtgccg cgcaatcgtt
    33421 gttattgatt gataaaagta aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga
    33481 tgaggtaacg cacattattt ctgatgagcg ccaggttgca acttctttgg taacagcctg
    33541 acggaaaggg tagcaggccg aagacgacgc cccggccttg cctgttatcg ctcgtcgcga
    33601 cgaccaccca ccgcagccca gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga
    33661 tacaactgcc gcgcctgaat ctgagcattt atgccatgtt catcaagctg tgcctgaata
    33721 tacgccagat tgtgtgatac ttcttcgtag cgttttttca tcggcagttt gagattgaaa
    33781 atggtttcac ggcaccagcc attaaccagc cactgcgcca tcaatgccgc aactttagcc
    33841 ggtttttcaa ccatatcgca taccatccag gagatattgc tgcgtgtcgg acggaatttg
    33901 aaaccgtctt cccgcagcca cgtcacctgc ccggtatcca tcagactttg cgccatcggg
    33961 ccgttgtcga cggaataaac ccacatgttg cgcttcacca gttggtaggt ccagccgcca
    34021 gggcaagcgc ctaaatccac cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc
    34081 gcaggaataa acacatgaaa tgcctcttcc agtttgagcg tggaacgact cggcgcctct
    34141 gccggaaatt tcaggcgcgg aatgcccata tagaacggcg aattattgtt gctgtatgag
    34201 taaccggtat agcagcagcc tggtgcaata aagaatacat gcacaaccgg acgcttcggc
    34261 gtttcatagt tcgccagcac cccggcatcg cgcaaggcag cgcgtagcgg cacggtgaat
    34321 ttacggcaga atttcagcaa ctctttgctt tcgttggtat cggcaacttc aacacgcagt
    34381 tcaccgccct tctctactac gccctgcaac atgtcgacaa tgggggtaat acgatcttct
    34441 ggcggcaaat gctgcaagag ttcccccacc acaaaccact ggcgggcaaa aattaatgaa
    34501 ctgaacggca gctcacggat taacttatcg ccatcatcag gttgataaca ttcataaatg
    34561 acataacccg cattctcttt cacgcgggca aaaccgaaaa tttcccgctg gccggcttta
    34621 tcggtaattt ctgcggcgca ctctttttca aagcccggac ggcacagcaa tacaacctta
    34681 ttcatgactt acgcccttgc gctttaaacg gatagcacca actaacatta acgcccagcc
    34741 cgcgaggaag ctcacgccgc caaccggtgt gacaaatgcc cacaaacgca gatgggacag
    34801 cgccaggcaa taaaggctgc cgctgaacaa caccgtgcct aacgcgagga aaacgctact
    34861 ccagtaaaac cagatactga tgcgacgctg cattgccacc gccagaccta agatcgccag
    34921 cgtatgaaac gcctggtatt cgaggccggt ctggatccag cccatctcaa cggcccccat
    34981 ggttttactt aacacatgcg cgccaaaagc gcccagagcc acaaaaatga agccgctaat
    35041 ggcggcgaaa atcagcataa aacggctggt catggtcgta ccctacgtaa attattgttc
    35101 ataacgaaag cggaattttt cttgttcagc agcggctttc gccaggatcc attggcgaaa
    35161 ggcggctatt ttacccagtt ctgcctggct gtcatgacaa accagataaa aagcgttttt
    35221 actgaccaga acatcattaa acgggcaaac aagacgtccg gcctcgattt cagattgcgc
    35281 catcacgtta tttgccagcg ccactccctg cccgtgaata gccgcttgca gcaccatggc
    35341 gctatgacta aaaattggcc cttgctgaac gttgatatga tttaacccca actgtcgggt
    35401 atatgtctgc cagtcacggc gtgacgcatc atgtaataac gtatgtttag ccagatcttc
    35461 cggggtcttc aagggttttt cgcctgtcag cagtagcggc gaacacaccg gcaataaata
    35521 ttcggcgtac agtttttcca cccgtagccc cggccagttg ccccgaccat aaaatatcgc
    35581 cacatcaaca tcatccgcca gcttatcttc ctgacgatca accgcctgga ttcgaacgtc
    35641 aattcccgga taagctgaat taaagctgga aagtcgcgga accaaccaat gaatggcgaa
    35701 actggggagt aaactgaccg tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt
    35761 cgcttcggtt aattgcgaaa atatctcttt gatatcgagg aaatagcttt gcccttcctc
    35821 ggtcagcagg agtgaacgat tacggcggcg gaacagtttt agccccaaaa aatcctcaag
    35881 agacttgatt tgatgactta ctgcggcctg ggtcacgaaa agctcttctg ctgcgcgagt
    35941 gaaacttaaa tggcgtgctg cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg
    36001 tttagacatg gcttttaaac tttgatgtta aattaattta acaattagat cacactgtgt
    36061 aacctattag tttttttaat ctgagccatt ataaattgtc cgttgagctt ctaccagcaa
    36121 atacctatag tggcggcact tcctgagccg gaacgaaaag ttttatcgga atgcgtgttc
    36181 tggtgaactt ttggcttacg gttgtgatgt tgtgttgttg tgtttgcaat tggtctgcga
    36241 ttcagaccat ggtagcaaag ctaccttttt tcacttcctg tacatttacc ctgtctgtcc
    36301 atagtgatta atgtagcacc gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat
    36361 tactgatcca gttcgaccat ctctttcacg tcagtacggt tgatctgctg tttgttgcca
    36421 ttagcgtctt tatacgaaat cataccggta tcgttatcag tctgtggttt gccgtcagag
    36481 acgatggtac gtccgtcatt ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg
    36541 gcaaaagtca gcatacaggc agaaataatt gcggcagtct ttttcatcat cttctcctta
    36601 aagcgattaa taaatcttaa tagtattgat taacaggcta aaattaacgc ctaacactat
    36661 tcagcatatg ttacttacgc ggcttcgcca ggatatccag ataattctga tggttagcac
    36721 tctccttgta tcaaagtgaa ttttgcgtca cgatcggtgc atcaagccga ggagtaccat
    36781 gaacgttttt aatcccgcgc agtttcgcgc ccagtttccc gcactacagg atgcgggcgt
    36841 ctatctcgat agcgccgcga ccgcgcttaa acctgaagcc gtggttgaag ccactcgaca
    36901 gttttacagc ctgagcgccg gaaacgtcca tcgcagccag tttgccgaag cccaacgcct
    36961 gaccgcgcgt tatgaagctg cacgagagaa agtggcgcaa ttactgaatg caccggatga
    37021 taaaactatc gtctggacgc gcggcaccac tgaatccatc aacatggtgg cacaatgcta
    37081 tgcgcgtccg cgtctgcaac cgggcgatga gattattgtc agcgtggcag aacaccacgc
    37141 taacctcgtc ccctggctga tggtcgccca acaaactgga gccaaagtgg tgaaattgcc
    37201 gcttaatgcg cagcgactgc cggatgtcga tttgttgcca gaactgatta ctccccgtag
    37261 tcggattctg gcgttgggtc agatgtcgaa cgttactggc ggttgcccgg atctggcgcg
    37321 agcgattacc tttgctcatt cagccgggat ggtggtgatg gttgatggtg ctcagggggc
    37381 ggtgcatttc cccgcagatg ttcagcaact agatatcgat ttctatgctt tctctggtca
    37441 caaactgtat ggcccgacgg gtatcggcgt gctgtatggc aaatcagaac tgctggaagc
    37501 gatgtcgccc tggctgggtg gcggcaaaat gattcacgaa gtgagttttg acggcttcac
    37561 gactcaatct gcgccgtgga aactggaagc aggaacgcca aatgtggctg gtgtcatagg
    37621 attaagcgcg gcgctggaat ggctggcaga ttacgatatc aaccaggcag aaagttggag
    37681 tcgcagcctg gcgacgctgg cggaagatgc gctggcaaaa cgtcctggct tccgctcatt
    37741 ccgctgccag gattccagcc tgctggcctt tgattttgct ggcgttcatc acagcgatat
    37801 ggtgacgctg ctggcggagt acggtattgc cctgcgggcc gggcaacatt gcgctcagcc
    37861 gctactggca gaattaggcg taaccggcac actgcgcgcc tcttttgcgc catataatac
    37921 aaagagtgat gtggatgcgc tggtgaatgc cgttgaccgc gcgctggaat tattggtgga
    37981 ttaatgacga acccgcaatt cgccggacat ccgttcggca caaccgtaac cgcagaaacg
    38041 ttacgcaata ccttcgcacc gttgtcgcaa tgggaagata aatatcgcca gttgatcatg
    38101 ctggggaaac agcttccggc tttgccagac gagttaaaag cgcaggctaa agagattgcc
    38161 ggatgcgaaa accgcgtctg gctgggatat accgtgactg aaaacggtaa aatgcacttc
    38221 tttggcgata gcgaagggcg cattgtgcgc ggcctgctgg cggtgctgtt gactgccgtt
    38281 gaggggaaaa ccgccgccga attgcaggca cagtcgccgc tggcattgtt tgatgagctg
    38341 ggattgcgtg cgcaacttag cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg
    38401 attatcgctg cgacgaagca ggtttaagca tagaaccggg tcggataaga cgttcgcgtc
    38461 tcatccgacc tgattgttta accctgacgc gccgctttcg ccatcatctt cttcagcgca
    38521 tgagaaaccg caacaaaccc aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat
    38581 gcacaatcca tccgcttcgg cccttctgcc gtggctttca tcgcacacac agtaccgtct
    38641 gactgcgggt acaccagcgc ttcagtagaa aacacgcaat cgacgccgag cttaccttta
    38701 ctgtttttca ctacgccaaa atcgcttttc aggcgctcgc gcaacttcgc cgccagcgga
    38761 tcctgaatcg ttttcgccag atcggtaacc tgaatctgcg tcgggtcaat ctgcccaccc
    38821 gcgccaccgg tcgtgaccag cgggatttta ttgcgccgac aataagcaat cagcgccgct
    38881 ttgggccgta cactatcaat ggcatcaatc acgtacgaat aacccacgct catatactgc
    38941 gctacgttat ccggcgtcac gaaatcatcc accaccgtta cacggcactc cgggttaatc
    39001 tggcgaatac gctccgccat gacttccgct tttgccagcc cgacgttatc gcgcagagcg
    39061 tgaatttgcc gattggtatt ggtgacgcac acatcatcca tatcgataag cgtgattgcg
    39121 ccaatccccg tgcgcgccag cgcttccgcc gcccaggaac cgacaccacc gataccaacc
    39181 acacaaatat gcgcgtcagc aaacagttgc aacgcttttt caccatacag acgcgctgtg
    39241 ccaccaaaac gctgacgcca tgcatcacta attaccacag acatagcacc tcagaattaa
    39301 aaagggtgag gttatccctc accctgtcgt atccgtaaaa acggtataca gaataacaca
    39361 tcagccgcta aagacgttac ctgcgcccgg ggcggttttc agcacccaga cacgtccata
    39421 gtggttgtac caacctgcgc ggtgtccggc ttccggcccg atcccttgat agatatcgaa
    39481 gtgttggcct ttgattgcac caccgacatc cagcgccacc atcagacgca gttcgtactg
    39541 accattaaat ttgccgttat tatccagcaa cggcacttct gccagcaagg tagtacctgg
    39601 cggaataatg gaacgatcag aggcaactga cgcgcgacca accagcggca ccgcacttgc
    39661 ccctttcacc ggtgcaaaag attgcggttt aaagaagacg aaagacgggt tctgttccag
    39721 cagctcgcga acctcggctt cactgtgtgt ttcgccccag tgacgaatcg cctgcatcga
    39781 catatcttct tttttcactt cgccacggtc gatcagcacc ttaccaatgc tgcgataggc
    39841 atgaccgttt ttccctgcat agctgaaaaa gttaagcgga ctgccatcac caaagtcgat
    39901 atacccacta ccctgcacat ccataatgaa gttatccatc agggagttac tgtaagcgag
    39961 aatatattta tcactcaatg cccccgcgta gatctccgca cgagacggca gacgaccacg
    40021 ttttggcggc atacggtaaa taggatactg gaactcgccc tggcgggtat ggcgcgcctg
    40081 aattaccggc gtgtaatagc ctgtaaactg cacgttacca tagttgtcgg caccttccat
    40141 ctgccaggca tcaatgccga actggcgcat attgcgggta tcaccgcctg cgcgcagcca
    40201 ctcttgcacc gcgttataaa cattactctg gttgccatac agacgcggtg acgaattacg
    40261 gatatggtta atttgctcgg caaaatcacc ggcgttaatc ggcgcgccaa cggcatctgg
    40321 ctggttcacc agagagaaag gctgggtaaa tttcccgtct ttatattgct gtccgcgatc
    40381 ggttggtttg gaagagcagg cggcaagcat tgccacaacc gtgcccataa gaaggtactt
    40441 tacccaacgt cctttcattg ttctcttctt caggttaaaa aataaggcgc aacgaagata
    40501 acaaaccgcc gcgtttaatg aaatgagcag cctctccctg acgcaaattt tgcacaaaaa
    40561 ataggcttta gtgatttgtt tttgttcaaa atcatgccag atccgtgatc ggggtaaaaa
    40621 aaaggttgca tgaaaacgcg agcggagtat agtgcgcatc cacggaggcg gggtggagca
    40681 gcctggtagc tcgtcgggct cataacccga aggtcgtcgg ttcaaatccg gcccccgcaa
    40741 ccaattaaaa tttgatgaag taaagcagta cggtgacgcg gggtggagca gcctggtagc
    40801 tcgtcgggct cataacccga aggtcgtcgg ttcaaatccg gcccccgcaa ccaatcaaat
    40861 ttgatgaagt aaaagcaata cggtgacgcg gggtggagca gcctggtagc tcgtcgggct
    40921 cataacccga aggtcgtcgg ttcaaatccg gcccccgcaa ccaattattg aacaccctaa
    40981 cgggtgtttt tttgtttcta ctctcccata aaaaaagcgc cattcagcgc ctttttatca
    41041 tccccttctc gccagcgttg ccccatcggc aaaatacgct ttaatccccg caagaataga
    41101 ctccgcaact tcctgctgga aagtcgccgt tttcagttta cgctcttcct caacgttact
    41161 gataaacgcc gtttcgacca gaatggaggg aatatctggt gcctttagta cggcaaaccc
    41221 ggcctgttca acttgatttt tatgcagctt gttgatttta ccgagcttat tcagcactgc
    41281 tttaccaaac ttaaggctgt cagcaatggt cagcgactgc accatatcga acatggtgtg
    41341 gtcgacatag cggtcaccgc ttttgctcac gccaccaatc aagtccgagg cgttctgggt
    41401 ttgtgccaga tattttgccg cagtactggt tgcacctttg gttgagagcg caaacaccga
    41461 ggaaccgctc ggctggcgac tggtaaaggc gtcggcatgg atagagacaa acaggtcagc
    41521 acgctgtttc tgggcttttg ctacgcgcac ttgcaacgga atgaagatgt cttcattgcg
    41581 cgtcatgtac accttcatat tgccctcttt ctcgatcaga gagcgcaaac ggcgagctat
    41641 ttgcaatacc acatcttttt cgcgcgtttt gtatttcccc accgcaccgg agtcttcgcc
    41701 accgtggcca gggtcaagca taatgacaat cggacgatcg cgccctgctt tacctggttg
    41761 tggaccactt tgcgctggcg gcacctgctt ttcgaggtcg cctttgttgt aatcctccag
    41821 cagcgccagc agcgggtcct gcatatcctg tgcattggcc ggatagaggt ccatcaccag
    41881 acgctcttta aacccggcga ccggcgcaag ggcaaacagc tgcggtttta cgttttgctt
    41941 taattcaaaa accatccgta cggtttgcgg gtcaaattgc ccgacgcgcg ccgacttgat
    42001 gaacgggtcg tctgcacgaa tttgtgccgc catccccttg agtaccgagt tcaggtttac
    42061 atcttcgata tccaccacca cgcgttcagg gttactcaac gcgaactgct tatatttcag
    42121 ctgacgattt gattctaccg tcacgcgggt gtagctggac gcaggccaga cgcgcaccgc
    42181 cacgacctgg ctaaccgcag ccaggctgac ctgacttacg ctcaatagcc acatagcgcc
    42241 cgcgccttgc agtaaacgac gacggctgat tgcagtgttg gatcctgaca tgcctctccc
    42301 gagcaaaaga aatctaagct gtgtaacaag taaacgacta atttgaccgg aaactttagc
    42361 gaaagacgca taatctgtca tctaataaac ggtaaacatt ctttttatat tcacggcatt
    42421 actgataaaa aagtcgctct cgcataaaat ttacacttgc accctgcgaa aaaacagaat
    42481 aaaaatacaa taatttcgaa taatcatgca aaggggtgtg ccgtggtaaa ggaacgtaaa
    42541 accgagctgg tcgagggatt ccgccattcg gttccctata tcaataccca ccggggaaaa
    42601 acgtttgtca tcatgctcgg cggtgaagcc attgagcatg agaatttctc cagtatcgtt
    42661 aatgatatcg ggttgttgca cagcctcggc atccgtctgg tggtggtcta tggcgcacgt
    42721 ccgcagatcg acgcaaatct ggctgcgcat caccacgaac cgctgtatca caagaatata
    42781 cgtgtgaccg acgccaaaac actggaactg gtgaagcagg ctgcgggaac attgcaactg
    42841 gatattactg ctcgcctgtc gatgagtctc aataacacgc cgctgcaggg cgcgcatatc
    42901 aacgtcgtca gtggcaattt tattattgcc cagccgctgg gcgtcgatga cggcgtggat
    42961 tactgccata gcgggcgtat ccggcggatt gatgaagacg cgatccgtcg tcaactggac
    43021 agcggtgcaa tagtgctaat ggggccggtc gctgtttcag tcactggcga gagctttaat
    43081 ctgacctcgg aagagattgc cactcaactg gccatcaaac tgaaagctga gaagatgatt
    43141 ggtttttgct cttcacaggg cgtcactaat gacgacggtg atattgtctc agaacttttc
    43201 cctaacgaag cgcaagcgcg ggtagaagcc caggaagaga aaggcgatta caactccggt
    43261 acggtgcgct ttttgcgtgg cgcagtgaaa gcctgccgca gcggcgtgcg tcgctgtcat
    43321 ttaatcagtt atcaggaaga tggcgcgctg ttgcaagagt tgttctcacg cgacggtatc
    43381 ggtacgcaga ttgtgatgga aagcgccgag cagattcgtc gcgcaacaat caacgatatt
    43441 ggcggtattc tggagttgat tcgcccgctg gagcagcaag gtattctggt gcgccgttct
    43501 cgcgagcagc tggagatgga aatcgacaaa ttcaccatta ttcagctcga taacacgact
    43561 attgcctgcg ccgcgctcta tccgttcccg gaagagaaga ttggggaaat ggcctgtgtg
    43621 gcagttcacc cggattaccg cagttcatca cggggcgagg ttctgctgga acgcattgcc
    43681 gctcaggcga agcagagcgg cttaagcaaa ttgtttgtgc tgaccacgcg cagtattcac
    43741 tggttccagg aacgtggatt taccccggtg gatattgatt tactgcccga gagcaaaaag
    43801 cagttgtaca actaccagcg taaatccaaa gtgttgatgg cggatttagg gtaagcgatg
    43861 aaaatcgccg gatgcggcag gcgtaacact cgtgcgtcgc atccggcaat tacgtttatc
    43921 cccgcgaact aaacaacgcc gccagaccac tgcgccgctc agtacgagtg gcgattgccg
    43981 cacttaatat acgctcatcg gcatacagcg acagacggcg acgcgcgcgg gtcaccgcgg
    44041 tgtataccag ctctcgcgtt actatcggcg tgcgttggct cggtaaaatc aacgccgcat
    44101 ggtcgaactc cgatccctgc gatttatgta ccgtcatcgc ccacgtagtt tcgtgctctg
    44161 gcaagcgact cggttgcaca gacttaatat tgccgtccgg catcgcaaac cagacgcgcg
    44221 tcccctgccc gcgatccagc gcaataccga tatcgccatt aaacaaccca agcgcgctgt
    44281 cattacgggc aatcatcacc ggtcgacctt cgtaccaacg agagtgcgaa tgacgatgaa
    44341 ttttgcgctt ctgttgcata aactgctcaa ttcgctcatt cagtccagcc acgccaaatg
    44401 gcccttcccg cagggcgcac aaaagttggt actcattgaa cgcctgaatg attaaatccg
    44461 gctcggcacg cgcttgcagc agatccagat aacgtccata acccgcaaga gcttcctcaa
    44521 gcatcgcaat ataatcttcg ccgctctgta aaagccgttt ttcaatatca gtaaaatcct
    44581 gctgaaaaac ggttttcact gccgttttat caccacggtt gatcgccgca gctaactgac
    44641 caatgccaga atcgctgccg aaacgatagc ttttttgcag caggcagaga ctgtcgcgca
    44701 aagatgccgc ttctgtgccg gttcctgccg gaacgtgagt tcccgtcagg cggcttagct
    44761 gcccggcacg ctcggcggta aagccggcat tggcataagc gcagatatcg cccagcacag
    44821 ccccagcctc aaccgaggcc agttgatcac gatcgccgag aaagatcact cgcgcatgat
    44881 cgggcaaggc gtcgatcagt ctcgacatca taggcagatc gatcattgac gcttcatcta
    44941 ccaccagcac atcaagatgc agcgggttac cggcatgatg acgtaaacgc tggctacccg
    45001 gctgcgcgcc cagcaatcgg tgcaaagtgc tggcatcttc cggaatgcgt ctcttttgtt
    45061 catcggtcag cggtaactgt cgcaaagcct tgccgagaga ttcggttaag cgcgcggcag
    45121 ctttacccgt tggtgcagcc agacggatac ggcagcgttc gccgtcggcc atttgaatta
    45181 acgctgccag caacttcgcc acggtggtcg ttttaccggt gcctgggccg ccggaaatca
    45241 ccgagatccg ccgcgtcagc gccactgctg ccgccacttt ttgccagtta atttcatcgc
    45301 ttactggaaa aagtttgtcc agggtttgcg ccagtagagc ttcatcaacc tcaatggcat
    45361 gattcacctc attgaagaag cgcgctaccg tccgctcgtt acaccacatg cgattcaaat
    45421 aaagacgatc gccacagagg atcatcggcg tgggttcatc tcccctgctg accgcttgag
    45481 aagccagcaa ggattcttcc caattttgta gctcaccgat ttcactgaca caggtcgcca
    45541 acagcggatg cgacgcctcg ttattttcca gtcgtgaaag cggcaaacag acgtgtccct
    45601 ctccggcatc atgacttaac agtgccgccg cgagggtgac ggcaggatgt tcatctcccg
    45661 ccacggtcag ggcaaattgc acatccagcg ggcgtagctg tttgtgctcc acagcttcca
    45721 gtaattgctt ttgcaatttc attacgcctc ctccagggtc ataccggcaa acatctcatc
    45781 catcagggca atcaacccgg cgttgggtcg ggttgtgtaa atcccttgtt gcggatgttc
    45841 tttatcaacg ccacgcagga acagatagat aacgccgcca aagtgacgat catagtcgta
    45901 atcagcaatg cgatggcgca gatagcgatg cagcgccagg gtataaagct gatattgcag
    45961 atcatagcgg tgtgcctgca tcgctgctgc catagcctgt tgggtgtaag ccgaactgtc
    46021 ttcacccagc cagttggatt tatagtcgag caggtaataa cgcccttcgt ggcggaatac
    46081 caggtcgata aagcctttta acatgccgcg tacctgcatg aactccagcg gcgggcagcc
    46141 agcggatagc gggtcaaact ggcgaattag tgcatcaagc tggctggcga tgagcggttc
    46201 gctaatcggc aggtaaaact ccatctccac ctgtttatcg cggtcggaaa gctgactcag
    46261 gctaacaccc gtttcattga gaggtgcctg gaggacagcc gtgatccact cggtcaacac
    46321 cggttcccac tgcgattcaa agccgccgag ttccagtttt tcctgtaccc agttcgggtc
    46381 aaccggctgg gtaaaatcga ggtcttcaaa caaactgtgc aagaatgtcc ccggtgacgc
    46441 accgcgcggg aactgatgcg gtgttaacgt cggttcttca acgacgctga ccacgcctgc
    46501 ggcatcaaca tccagccgtg gcatcagatc ctgagcgata ccgtgaccac gctgctgcaa
    46561 gccggagtag ctggtgacac gccagttatc gccgggcaat cgttgtagtg tcctcgcatt
    46621 cagttctgca gttaaagcat cattaacctg ccagggctgg ttatcaccag tttgtgccgt
    46681 ttgccaggca atatcatcat cgcataacgc ttcaatacag gtgcgaagcc ctgccgcatc
    46741 ttgcggttcc cctttttgca gcaaacgccc gagcgcactt tggtggacgt cggtgtcacc
    46801 ttttttatcg ccacgacggc gcaccagcgg tgcaacgccg agactgcaat gccaaaccga
    46861 acgcgtcagc gccacgtaaa gcaaacgcag atcttccgcc agacgttcga cctccgcgag
    46921 gtcgacgctt tctggcgcag cattaagatc caaaactgcc tcaaacgagt ggcgatcgtg
    46981 ataaaacgcc tgatcctgga cgcggaaatt ggtaataaat ggcaaccaga ccagcggata
    47041 ttccagccct ttcgatttat ggatcgtgac aatctgcacc agatgtttat cactttcgag
    47101 gcgcatttgt tggctggagg cattactgtc tggctcgagg atatgttgcg ataaccagcg
    47161 taccagcgca tgttcacttt ccagctgcgt tccggcttct tgaagcagtt cgctgatatg
    47221 caagatatcg gtaagacgcc gctcaccgcc tgccgttgcc agcaagtttt ctgcaatgtt
    47281 acgcgccgac atcagcgccc gcagcatcgg cataacgcca cgtttgcgcc agatttgccg
    47341 ataaccatcg aactcttcga ctaccgcatc ccacgcatgt tcgtcattat tcagcgtttc
    47401 aatatccagc gcattcagcc ccatcattga cgttgccagc gcactgcgca gggtgttctc
    47461 acgttcgggc gtcatcaccg cctgcaacag ccaaagcatt tcctgcgctt ccagagtttc
    47521 aaaaacacta tcgcggttcg aaaggtaaac ggaagggatt tccagcaacg ttaaggcatc
    47581 gcgcacctgg gcggcctcct ggcggctgcg caccagcacg ctgatgtccg aagcacgcac
    47641 cggacgcgcg tcgtcgccgt tcatcagcaa cgcttcgccc cgctgtccgg cttgtagcca
    47701 gtcgcggatt tgcgcagcac atacctgcgc catggtactt tgatagtcgc caacgccgca
    47761 gctttcgcct tccatcagcc acattttcat cgcaggctgc gtttcacctt taaagacaaa
    47821 acgtaacgcc tgatttttcc cggctgattt cactggaata aacggtattt cgcgaaacat
    47881 gaacgcgtca tcagtctggc tgaaaagctt gttcacactg ttcaccattc caggtgcgga
    47941 gcgccagttg gtgtctaaag tgtagtgggc gtgaacttca ctacgcgcct tcatataagt
    48001 gaagatatcc gcaccccgga atgcatatat ggcctgcttc gggtcgccaa tcagcaacaa
    48061 tgcggtttct ggctgatggt gccagatacg gcgaaaaatt cggtactgct gggggtcggt
    48121 atcctgaaat tcatcgatca ttgccaccgg gaatcgcgta cggatcgccg ctgccaacac
    48181 ctcgccgctt tcgctacgca gcgcggaatc gagccgactt aacatgtcat caaaacccaa
    48241 ttcgccacgg cggcgttttt cacgcgctac tgtttcgcgg atctcagcca atgcgcgggt
    48301 gatcaccaga tcacggatcg acaatggttc tgcaagcagt tgatcgatcg cctcaaacag
    48361 tggatgtcgc ggggtttccc ccccggcttt cgtgcgatct tctaagaaac gctgggagaa
    48421 tttttccagc gactccggca actgataact gtttgtctct tcttctgccc aggcgctgat
    48481 cttgtcgatc catttagcct gattgctacg gttaaacttg cgtcgatcaa taccagaaga
    48541 ttcgatcagc gcatccagtt cacccactgc gtcgcgccac tgctgtttta ccgtatcaat
    48601 acgcgccaca atttgcgcgt gacgggaagc tagcgtttca tcatcgggcg gcggtgcttt
    48661 gataaccggc gcttcgcctt gcagataacg attaatatcg cgcagcaacg cctgcggccc
    48721 tttccaggtt tcaaagacga cctgggctat ttcacgcggc agcgggtagc agtggcgacg
    48781 ccagaaatcg gcgcaggcct ggtagcgtag cagagactca tcttcaatca gctgctgctc
    48841 aaacagcatg ccggattcaa aggcattcag gttgagcatg cgctggcaaa agccgtgaat
    48901 agtaaagact gccgcttcat ccatctgccg ttcggctaac aacaaccact gcgcggcttg
    48961 cgctttatcg tcgacctctt ccagcaggtg ttcgtacaat ggattgtcgg tggtttcacg
    49021 cagacaggcg atgcgcaact cgtggatatt gctacggata cgaccgcgca attctgccgt
    49081 ggcagcctcg gtaaaggtga ccaccagcag ttcttcaacg gtcagcgggc ggggaaaggc
    49141 ggcggaaccg cctagtccaa gtaacaggcg caaataaagc gccgcaatcg taaaggtttt
    49201 gcctgtgccg gcagaggctt caatcaggcg ctcgccctgt aagggcaagc gcaaaggatc
    49261 tagtgtctcg gcgacatcac tcattctttt cactcatcag gggcattgtt tgctgcaacg
    49321 cgctgacgtt ctcccacact ttccagcctt caggatgcac atattcggct ttcccgtttt
    49381 ggctgccgga aatctgcgac aggatagcca tgccttgcgg ctcgactacc gcctgatgga
    49441 agaaatcggc cagtttttgc ggcgtcagca gtttaatctg ggccacgatt ttatcacgcg
    49501 aatcgaagcg catattgccg cgatcgaaat ctttacttaa cttcgatgct tcttcgccga
    49561 gcgtttgcgg tgcctgcagc atctgggtaa ttaccgcctg ctggatttgc gcaaactcat
    49621 ctggcttcat cgctcgcaat tttgcctctg cggttgggaa aaacgccttg taacgctccc
    49681 acaagaatga aggctgttta tcattgcttt gcaaaaggaa gcccatgccc cactgacgcc
    49741 ccacgctcat tggaaacgca aacacggcat agcccaattg ttcttcggta cgcaactgat
    49801 tgtagaacca cggctgtacg atctgcccca acagagagct ataggctgag ctggtgtatt
    49861 catcgtagcc agtcggtaca aataccgctg ccagtgcgga gtcggtgctg ttaccggctt
    49921 tttcaaagat gacggattgt tttttatcga ccactacatc tttgtttcga caccactctg
    49981 aaccatcagc gcccaactgt ttttgcacat cgcgtgccag cgttgttgcc tgggcctcgg
    50041 tcatgttgcc gataaccata aactctggtc gagcccctga ttttaaggcg tcgcgataag
    50101 ccagcacctc tttcaacgta atggagggca aaattttacg ccgttcatct cgcgagaagt
    50161 acggcacttg cgagagcatc tgcgcgggca taatcgcctg ctcaaacgct ttaccctttt
    50221 ctgcggaatc catcatctgg ttataccagg acttcgcctg ctcaagctga tcttccgtag
    50281 cggtatagct aaagtacccc tcgagcaatg cctggaacag ctgcggcaga cgctgggtgt
    50341 aaccattagc attaaccata aggccgttgt tagcgttggt ggaaaaactt atgccaccaa
    50401 ccgacgcctg gttgcttaac tgatcaagcg ccagccctgc gagataatca ttgagcgcaa
    50461 acatcacctg attgcgggcg ctgtccatgg ctttcggatt acgcaaaatc aggctgacat
    50521 cagctttggg ttcgctggaa aaataacggc ttggcgcata caccacgcgc agattcgact
    50581 catcaacaat cagctctgga tggtcatatt tcttctctga cttaatcagc gagaaatcgt
    50641 caggaatata ggggttaagc tccggtaagg agagcgcaat attggcagct ttttgctgcc
    50701 agtcagcgaa agtttgttcg ctgattttat cgacctgata cggcgcatcg acaaaataag
    50761 ccgttttatt gtgcggctct ttcgggctga tataccagat acgcgcattc tgcggcgtca
    50821 tcatcgccag acgttctttt actgctttag catcgtaccg atcggcaata ttgactgcat
    50881 ccagcgtatg ctcaaccgga acgcgaatca tggtatctgc cagccattcg acgtaatcca
    50941 tatcacgggt gatcgacgga taacggaagt cgatatccag cacattcgcc agttcatcga
    51001 agtattgttt atcaatgcct ttttcacgta acagattgag atagctaaaa attgccgcca
    51061 caacctgatc gcgattagcc aggcctttat cggttaaaga cgcagagatc gctaatacgc
    51121 cgctgttgcc gttgacgata ggatcggagt tggcgctaat gccctcaact aatccctgct
    51181 tttgcagcca gtcggaaagt gtacctgggc tgcgattacc aatcagatag gtaatcaatt
    51241 catccgtttt actacggaac ttcgctgagt tgttatcgat gcgaaactca acgcgcagaa
    51301 ctttacgcgg cattgccggg acgtaatgaa tgataatgcc cttttgcgcg tcggtgacta
    51361 ccggcacggt gatttccggt tttttgctct ctttgttcgg cacgcgacca aaggtgtccg
    51421 ccgccatttt tgccaactcc ggcagcggtt tattgctgta aataaccgcc ttcatcagat
    51481 tggcggaata gtacttctcg tggaaatctt tcagcgcctg ctgcaccgga ttaccaggtt
    51541 tatcgcttaa agtttcgagg ttaccaccag aaaactttga accggggtgt gccgggttaa
    51601 tggtttctgc gctgacctgt gccatgcgca tcccgtcacg cgtacgcgcc atggttaatt
    51661 cagcgttcac cgcattacgc tcacgttcgg catatttctt gtcgagcaaa ggttcagcaa
    51721 tagcatcggc caggcggtct accgcaccag gcaaggcgtc gttctcaact tccagataga
    51781 aagccgtgcg atacggcgca gtgctggcat tgtgactacc gccgtgcatt ttgagatatt
    51841 cggccagact gtcagcctgc gggtactttt tcgaccccat cagactcata tgttcaaggt
    51901 aatgtgccag cccctggtac gcctcgggat cttccagcga cccaacgggc accaccagcg
    51961 ccgagagcga tttaactgcc tgcggatcag aaaccagcaa gaccaccata ccgttatcca
    52021 gacgtatagc ctgatactgg cggttatctt tatcactttt acggatggtt tcctgaatcg
    52081 gctgccatcc cgtttctgcc tgacttaagg gtgcccaaag ggcaactaac aacaataatg
    52141 ctttgaacca ggtgctgcgg ggcattctcg gacctcataa tcaacttaat tttctgtcca
    52201 gattcaacac gttaacgcaa atcatctgcc agaatttaat cttgtgctgc acgagtcagc
    52261 ctatgtttat ataaccatca gtccgtgact ggtgcgcatc ataaagtaag cggatagatt
    52321 gcgcaatttt tatacagcac tcatgactga ttaaagcgaa acagcggtaa caggaaacgt
    52381 tgcgactgtt caacgatggc ctccattgtc tctggtgtta attgccgcca gagcctttga
    52441 taccagatat catcaccttc gccacgcacc atcatgttac cttcgtaagc ctgaaggaat
    52501 ttcgtacggg ctttttgcaa cgtggaatcg tcatccagca tggcatcgtt ttgcgcgtca
    52561 taacaggttt ttagccacgc gccgccactt tcaggtaaca ccagcaatgg cgcggacatc
    52621 ccttcacgat acccctcaat cagttgtgag aggtaatgca aagcctgttc ggctgcaagc
    52681 ggcggaaaac gccactcgcc gtctttgcgt agaaaaaggc gactttcacc attaccaccg
    52741 ctggcacagt agacaaggtg ttccagccaa agttgcattc cctgcgccac acttaataaa
    52801 gagggacgcc agcgcaacag gccatccggc tgcacctgcg gcaaccagcc agttatctgc
    52861 acaccgttgc aggcgagatc aatttccata ctctgccccg gctggcgaca ggcaatgact
    52921 ctgtcggcaa gctgctgcat ctcctggcac tgagtttccc agaaaatttc accaaaagcg
    52981 ccatacggta aatcccctgc cgcccggaag cgacggaaca agcgttcggc atcatcctgc
    53041 tcaaccagtg cattcaataa ctgctgattg atttgataac ggctaagccc ttccagaata
    53101 aatggctcgg tgtcggggat ttcgctgtct tcagtacgga agttcacctg caaacgcatc
    53161 tggaagaacg cccgcaccgg atgtgcccag aatcgttgta gcgtttccag cggcacggtt
    53221 tccggtaagg taaacggcag cggctgaaca aattcagaat gtgctttacc agcctggctg
    53281 gccgcaggta gccattcacg agcatagctt tgtcgttcgc ctggctgata gttttgtgga
    53341 tcaaacggca ttcgggtatg gaggcaagta agatgcgctt ttacccttgc ctcgctttca
    53401 tcacagttga gcgcttcatc gcccggtaga taatgacttt gcccgatgta gtcgatcagt
    53461 tcctgcacca gtaccgacgg gaaacgttca ctgttatcct gaatggaacg cccgatatag
    53521 ctgatataga gtttttgctg cgcggaaatt aacgcttcca ggaacagata gcggtcgtca
    53581 tcgcgacggc tacggtcgcc acgcttcggt ttctggctca tcaggtcaaa gcccaatggc
    53641 gcaagctgac gtggataaac gccgtcgttc attcccagca ggcaaaccac tttgaacgga
    53701 attgaacgca ttggcatcag agtacaaatg ttaaccggtc cggcgagaaa acgctggctg
    53761 atacgttctt gatccagacg ctgtgccagt tcatcacgca atagtgacag cggcaccgcg
    53821 tcgccatact gcgcacctaa accttcggcg ataatcgcct gccattgttg ttcgatcagc
    53881 gtcatcgccg cttcggtttc cgcatccggc aggaagaagg cgctgagcat atcgcgacaa
    53941 accggcaacc actcttctag cggacgctcc tgtgccagcc cgcgacgcca gatgtttagc
    54001 tgcattagca gtgaagccag atgccccacc agttctgcaa ttaagccgct cgattcatca
    54061 taaggtagaa ccgattgcca ctcgccctgc gcgctctcca tcgcgtagcc cagcaacata
    54121 cgcgtcaggc caaatcgcca ggtgtgttgt ccggtggcgg ggagttccag ctcgcgaacg
    54181 ttgtcgtcat ctatccccca acgaatgccg gattcgttga cccactggcg taaataacgc
    54241 agcccttctt cggtgatgtc aaaccgcgcc gccagcaccg gcacatccag taatgccagc
    54301 acgtcttccg acacaaagcg gctgtctggc aacgataaca ggctgataaa cgcttccagt
    54361 accggatgcg actgccgcgc ccgacggtcg gaaatggcgt aaggcaggta acgatccgca
    54421 ggtgcgctac caaacacagc ccgaataaac ggactgtagc tgtcgatatc agccaccatc
    54481 acgatgatgt cgcgcggagt aagtgtcggg tcttcttcca gcatcgccag caggcgatcg
    54541 tgcaaaactt caacttcacg ctgcgggcta tggcaaacgt ggaaggtgat actgctatcc
    54601 agtggatcaa gcgggcgttt gttatcgcta cgggaaaact cttcgatgtt cacaccagca
    54661 acggcacggt tttccagttc cagaatgtca gactgaatgt tatgcagcag gttatctggc
    54721 gtgacatcga caaaggcgtc cagctcctgg ctgctctcca ggtcagaaag gagataaatg
    54781 tagtcgcgcc caagcttacc ccatgaagcc agcagcgggt tgccgacatc ctgttcacca
    54841 tcgctgttaa agagctgccc ggcattttcg ctatcacgaa atagcggcaa ttcgcgatct
    54901 tcaaaactgt gtcgacgctg acgggtcagc agtttcgcca gataagcagg atctttaata
    54961 tcgccccagt aataacggca ggggttggta aacaggagat ggatttcaat atgtttaccc
    55021 agcgcctgta gcgcctggag ataaacaggc ggtaacgcgg aaataccgca tataaagacg
    55081 cgcgaaggta accccggcgg gcaggtcgtc gcggactcca gcgtttcgat aaagcgctga
    55141 tagagattgg cgcggtgcca gcgcggttgc ccgagttgat gggtatattc caccagcgcc
    55201 ttccacaacg gggcttgcca ggcctgtgct tctcccagcc cttcaaccaa atgtcctgtt
    55261 tcccactgtg ccagccagtc cggacgatag accagatact ggtcaaacag gtccgccgct
    55321 tttgaggaaa gctggaacag ttttcgcttg tcgctatcgt cagtcagata atgccgcaac
    55381 agggtaaagt cttcgcgctc cagcaattgc ggcagcagag tcatcagttt ccagctcatg
    55441 ctctgtttgt taaaggcgct ctctttgggg atttccggta acacccggac gaacatatcc
    55501 cagataaagc tcgctggcag cggaaaatca atgtttgccg caatactaaa cttttgcgac
    55561 agggtcattt gcagccactg cgccataccg gtactttgca ccagaatcat ctctggttcg
    55621 aaaggatcgt ccagccgttc gcgttcgaca ataaactcca tcaacgcttc cagcacgtcc
    55681 agacgattgg aatggtagac ccttaacata gcggctcctg actactgacg attcgggcaa
    55741 tgcaggcggg tcatctcgcc ttctctgccc ccgggtgaaa ctagcgtaac gctgatgctg
    55801 acacatcccg cctgcgatgt ctgcattcgg ttgacctgcc agttggcagg tggcgaaatc
    55861 gcgcgcagtt gcgtttgctg ccaaccatgc cgccagagct gttggtactg gtttctgctg
    55921 gcgagactgt tcattaatgt tcgctggata cccgataacg cagtgacaat catcaccatc
    55981 agcaccatcg caaccattac ctccggcagg ctaaagcctt gttgattctt cagggaagct
    56041 gacataacgc cctctctttc agcggacaaa aatcgctcca gccgcgtggc gaaaaaacaa
    56101 tgtttccatc aatgacttcg cctgttcgcc acaacgaaac gccttcataa ccggcaacca
    56161 ataaggcttc attatctgcc agtaaacgca aacaaacccg ggcatcggtt ccagcgtact
    56221 gcgagcactg aactgcgggc tgcgtctgcc aggagtgcat tttcccccac gccagcgccg
    56281 actgaacgat ggcctggcgg cgcaatgact gactttctat gctcacgcga gaggcaaaac
    56341 tgcgatcctg ctgactcatt ccttgcaata gcaagctacc caaaaccagc agcatcagga
    56401 ccagagccag tgacgaaaca cctttttcgc ggttcacagg ttgaatcctg tcacgctata
    56461 gctggcatcc accacggttt gcggttcaga cttactggca gcacgcatat taaccgtcaa
    56521 caccggcgag aagccgctga catcctgacg tacgacctga aaagtgtcga taatgatggc
    56581 atccggatta gtgactttat cccagccctt accttcacag gatgtcgcac cgcgtagcgt
    56641 ttccagcaca tgctccttca gacgaaatcc aatctggtca gactctttta ccggttcgcg
    56701 atcccagata ccgttactgt tcgcatccca ctgcacaatg atacagtcac cctgtccgac
    56761 aatttccagc ccttcgccgg tacagctgcc atgacaataa cccgccctct ggagatgctt
    56821 cgcgacggta aatacccgca gccagatttc atcttccagc gccagcttac gggtgctcgt
    56881 taaactttca cgctgtaacg caggcagaaa gcgtgcagcc cccagcaaca atacgctact
    56941 gatcgccata gcaatcaaca cttccagcag agaaaaacct tgctctttta caggcatcct
    57001 tctgtttctc cttgctgaca aagccggagt cttccccacg gcgaaaccac cagccaccac
    57061 tcgcccgttg agtttttgaa gcgaatatgc ccggcccatg cggtattgcg caggccaaag
    57121 aaagcaagcg aaggtgtcag gtcgctcatt tcgacttcgg gccagcgtgg cacaaagacc
    57181 aatggtgaac tgccatgaca ggtattggcc ccagcagcgg aactcacaag gcaccataac
    57241 gtcccctccc tgataacgct gatactgtgg tcgcggttat gccagttggc atcttcacgt
    57301 aaatagagca aatagtcccg cgcctggctg gcggtttgcc atagccgttg cgactgctgc
    57361 cagtattgcc agccatagag tccacttgcg cttagcatga ccaaaatcag catcgcgacc
    57421 agcgtttcaa tcagcgtata accacgttgt gttttcatgc cggcagtatg gagcgaggag
    57481 aaaaaaagac gagggccagt ttctatttct tcggcgcatc ttccggacta tttacgccgt
    57541 tgcaggacgt tgcaaaattt cgggaaggcg tctcgaagaa tttaacggag ggtaaaaaaa
    57601 accgacgcac actggcgtcg gctctggcag gatgtttcgt aattagatag ccaccggcgc
    57661 tttaatgccc ggatgtggat cgtagccttc aatctcaaag tcttcgaaac ggtagtcgaa
    57721 gatggattcg ggtttacgtt tgataatcaa cttcggcagc ggacgcggtt cgcggcttaa
    57781 ttgcagatga gtttgatcca tatgattgct gtacaggtgc gtatcgccac cggtccagac
    57841 aaaatcaccc acttccagat cgcactgctg cgccatcata tgcaccagta acgcgtagct
    57901 ggcaatgttg aacggcaggc cgaggaagac gtcacaggag cgctgataaa gctggcaaga
    57961 gagtttgccg tctgccacat agaactggaa gaatgcatgg cacggtgcca gcgccatttt
    58021 atccagttcg cctacgttcc acgctgaaac aataatgcgg cgggaatccg ggtcgttttt
    58081 cagctggttc agtaccgtag tgatctggtc aatatgacga ccatctggcg ttggccaggc
    58141 gcgccactgt ttaccataca ctggtccgag gtcgccgttt tcatcggccc attcgtccca
    58201 gatggtgaca ttgttttcgt gtagataagc aatgttagtg tcgccctgca gaaaccacag
    58261 cagttcgtgg atgatggaac gcaggtggca acgtttagtt gtcaccagcg ggaatccatc
    58321 ctgcaggtta aaacgcatct gatgaccaaa aatggaaagc gttccggttc cggtacggtc
    58381 gtttttctgt gtgccttcgt cgagcacttt ttgcatcagt tctaaatact gtttcatggt
    58441 tcctcaggaa acgtgttgct gtgggctgcg acgatatgcc cagaccatca tgatcacacc
    58501 cgcgacaatc atcgggatgg aaagaatttg ccccatgctg atgtactgca cccaggcacc
    58561 ggtaaactgc gcgtcgggct ggcggaaaaa ctcaacaatg atgcgaaacg cgccgtagcc
    58621 aatcaggaac aagccagaga cagctcccat tgggcgcggt ttacgaatat acaggttgag
    58681 gataataaac agcaccacac cttccagcag cagctcgtaa agctgtgatg ggtggcgcgg
    58741 cagcacaccg taagtgtcga aaatggattg ccactgcggg ttggtttgca gcagcaaaat
    58801 atcttctgta cgggagcctg ggaacagcat ggcaaacggg aagttcgggt caacgcggcc
    58861 ccacaattca ccgttaataa agttgcccag acgcccggca ccaagaccaa acggaatgag
    58921 tggtgcgata aaatcagaaa cctggaagaa ggaacgttta gtacggcggg caaagataat
    58981 catcaccacg ataacgccaa tcaggccgcc gtggaaagac atgccgccgt cccagacacg
    59041 gaacagatac agcggatcgg ccataaactg cgggaaattg tagaacagaa cataaccaat
    59101 acgtcccccg aggaagacgc cgaggaagcc cgcatagagt aagttttcaa cttcattttt
    59161 ggtccagccg ctgcccggac gattcgcccg tcgtgttgcc agccacattg caaaaatgaa
    59221 acccaccaga tacatcaggc cgtaccagtg aagcgccacg ggtcctattg agaaaatgac
    59281 cggatcaaac tccggaaaat gcagatagct actggtcatc tgtcaccaca agttcttgtt
    59341 atttcgctga aagagaacag cgattgaaat gcgcgccgca ggtttcaggc gctccaaagg
    59401 tgcgaataat agcacaaggg gacctggctg gttgccggat accgttaaaa gatatgtata
    59461 tgatccgcgc tataaccctc cgcgaatcag cccgcccatg ccgcgacgct ccataaaggc
    59521 tgcaacctga tggcgaactt cggtcgccag ttgcgcttcc agactacgct gcgcaagatt
    59581 ttctgcttcg gcataatcaa tgcgccgcag caggtatttt acccgcgcta cagaacgtcc
    59641 gttcatagac agatggcgat acccaagccc aatgaggatt gccacgcaca tgggatcgcc
    59701 cgccatttca ccgcacaaac ggagatcgat tccatgtatt tccgcttccc gggcgatcat
    59761 cgccagagct cgtaacattg caggatgaag actgtcataa atgttcgcca cccgggtatt
    59821 gttgcgatca acggccagaa tgtattgagt cagatcgttg gtgccaacag agatgaaatc
    59881 gacccgcttt gccagatgcg gcagcataaa taccattgac ggcacttcca gcatgatgcc
    59941 gatacgtggt ttgggaattt cgtaaccgat catctcctcg acttcacgtc cggcacgttc
    60001 aatcaggcgg cgtgcttcat caacttcatc gaggcttgtg accatcggca acagaatatt
    60061 caggttgccc gtagcggcat tagcacgcag catcgcccgc acctggatca agaagatctc
    60121 cggctgatcg agcgtaatgc gaatcccacg ccaacccagg catggattct cttcgctgat
    60181 cggcatgtaa ggcagctgct tatctgctcc gacatccagc gtacgcaagg tgacgggttt
    60241 atcattgaac atttgcagca tcccctgata ctgcgccacc tgttcttctt ccgacgggaa
    60301 accactttgc agcatgaatg ggatttcagt gcgataaaga ccgatgccat caatacggct
    60361 gcccagtttt tcttcatgtt ccgggcttaa accagcattg agcatgactt ttatacgctc
    60421 accgcttttt aactgcgcgg gtaaattgac gtcatcttcc gccagacggc taagctcaat
    60481 ctcttcacta attagccgct gatattcttg cagcagtacc ggctccggat cgaccagcaa
    60541 ttcaccgcga tagccatcaa cgatcagcgt cctacgatgc agcaccgaag gctgaatatc
    60601 cgcgcccatc acggtaggga tccccagcgc acgtaccatg atcgcagcat gggagttggc
    60661 tgcgccatct cgcacgacaa caccgactaa gcgatcctgg ggcagctcag caagagttgt
    60721 cgctgacagt tcatctgcca ccagaatgaa acgttccggc caggcgttcg gcccttgatt
    60781 agcgtcatca agatgaaaca gcaatcgctg acccagcgca cgtaaatcgc cagcccgctc
    60841 tttgagatag ttatcgctta gcgcggcaaa ctgttcggca aatttttcaa tgaccgtttt
    60901 taccgcccac tctgccaccg agcctttatc aacctcggca aacaattcgc gacgcagccg
    60961 ggtgtccgaa agtaggtgcg agtaaagatc gaaaatagcc gccgtttctt tttgtgctcc
    61021 ggcggcaaag cgtttgctgt agcggcgaaa ctcgtttgcc gcttcttcca gcgccccggt
    61081 cagtcgttcg cgttccagag ccggatccag cgttgatgcc tgatacacct gttccattaa
    61141 aggtaacgtg gcatcctgcc agccttcggc aatcgccaca ccaggtgctg ccggtaatgc
    61201 gcggattcgc gtctggcgat attgcccaaa caaggcagtc aactgcgact gagaaagaat
    61261 agctgccatc tgggtggcaa gcgtcaccag gaaggattct tcactttcgt catactggcg
    61321 caactctcgt tgctgtacca ccagtacacc aagcaactgg cgacgttgaa taattggtac
    61381 gcctaaaaac gcgcggaaac gttcttcttt tacggagggg atgtatttga agctggggtg
    61441 cttttgcgca tctgcaaggt ttatcggttc cgccagcctg ccaaccaggc cgacgatccc
    61501 ttcatcaaac gcgagcgtta cagtgcgacc gcgtggtttt ttcagccccc gggtcgccat
    61561 caggtagtaa caacgtcgat catgatcggc caggtagacc gaacagacct cggtatccat
    61621 cgcaagacag atgtcggtaa ccagaatatt taacgcctca ttcaggcgtg gtgcgctggc
    61681 taccttttcg actatttcgc gcaggcgagt gagcataatt ggcgtgactt aacctctttt
    61741 acgtcgataa gcagatgtgt tttgtggttt tggcgtattt tcctgcagtg acatcaccac
    61801 actcgcgaac tctttcatta ccctacggta gacatcacgt ttaaatgaca ccacctgtct
    61861 gaccggatac cagtaactta cccatcgcca gccgtcaaac tctggtgtac tgctggtttg
    61921 catattgatt tctgcatcgc cgctcaccag ctgcaagaga aaccattttt gtttttggcc
    61981 gatacaaacc ggcttcgtgt cccaacgcac caaacgtttc ggtaatttgt agcgcaacca
    62041 gttacgcgtt gaagcaagga ttcgaacgtc tttgcggctt aatcctactt cttcaaacaa
    62101 ttcacggtac atcgcctgct ctgcggattc tccggggttg attccgcctt gcggaaattg
    62161 ccaggagtgc tgaccaaatc gccgggccca cattacctgc ccctggcgat tacaaatcac
    62221 gatacctacg tttgggcggt agccatcgtc atcaatcacc ggactacctc aaaataaagc
    62281 tttatatacg aatgattgtt tcatactcca agaagacggt aaaccactct ctgcagggca
    62341 ttacacacta ataacaattg aataactcac agttatgtgc agagttataa acagaggaag
    62401 gggtggatag ccgtttttat tcaccttttc tgtggataga gttgtgaaga actacggaat
    62461 tactacggga aaacccggag cattctgaat aagcccgata tagacatttt aaaatattca
    62521 ttattagtca atgcattaca tgtaatttga gtaatgaaaa agagtaattc gtgacccagg
    62581 tcacacctct catttacggg ttggcgaaag atcaaccaat gccgtattta tccacagaat
    62641 gtgccactaa gttaagcact gaaccactaa aaactggagt ttcgtcgcac gtcaaggctg
    62701 taaatggaaa cagtagtgga ggtttttcac agttatccca gctttctgtg gataacatgg
    62761 tgtaagatcc tgtttatttt cagtgaccag atttggaaaa cctattgcag tgttgcgcaa
    62821 ctcgattacc ggcagcctaa aaagcaatat aaatcagaga attgaacaac acatgtggaa
    62881 aaagttacac tgcgaatatt cggcacataa ttgctgtttg ttttttaacc aaggtatcat
    62941 gacatgtccc aacctcgccc actcctctct cctcccgaaa ctgaagaaca gttgttagcg
    63001 caagcacagc aactttctgg ttatacattg ggagaactgg cggcacttgc cgggctggtt
    63061 acgccagaga atttaaaacg cgataagggc tggattggcg tgttactgga gatctggcta
    63121 ggtgccagcg cagggagtaa acctgagcaa gattttgctg ctctgggcgt ggaacttaaa
    63181 actatccctg tggatagtct tggtcgtccg ctggaaacaa cattcgtttg tgttgccccg
    63241 ttaacgggta atagcggggt gacctgggaa accagccatg tgcgccacaa gctcaaacgc
    63301 gtactgtgga taccggttga aggtgagcgc agcatcccgc tggcgcagcg tcgcgtagga
    63361 tcaccgttgc tgtggagccc gaatgaagag gaagaccgac agctacgcga agactgggaa
    63421 gaattaatgg atatgattgt tctcggtcag gttgagcgga tcaccgctcg tcacggggag
    63481 tatttacaga tacgaccgaa agcagcgaat gcgaaagcgc ttaccgaagc cattggtgcc
    63541 cggggcgaac ggattctgac gctgccacgc ggcttttatt tgaagaagaa tttcaccagt
    63601 gcactactgg cccgtcattt tctgatccag tagccatcgc tttgacctgc cgctttccgg
    63661 gcatataatt accgcttcat ttttttggca gggcttttta gatgttattt gcatggataa
    63721 ccgatcctaa cgcctggctt gcgctcggta cgctgacgct gctggagatc gttcttggga
    63781 tcgacaatat tattttcctt tctctggtgg tggcaaagct ccccacagca caacgtgctc
    63841 atgcgcgccg tctggggttg gcgggagcca tggttatgcg tctggcgctg ctggcatcaa
    63901 tcgcctgggt tacgcgcctg acaaatccgc tttttacaat attcagtcag gaaatttccg
    63961 cccgtgattt gattctgctt ctgggtggct tgttccttat ctggaaagcc agcaaggaaa
    64021 tccacgaatc cattgaaggt gaagaagaag ggctgaaaac acgcgtttca tcattcctcg
    64081 gcgctatcgt gcagattatg ctgctggata ttatcttcag cctcgactcg
//
LOCUS       DAJVQG010000005        60958 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJVQG010000005 DAJVQG010000000
VERSION     DAJVQG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.005"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..468
                     /locus_tag="OQM83_000289"
     CDS             <1..468
                     /locus_tag="OQM83_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1518648.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            581..1375
                     /gene="panB"
                     /locus_tag="OQM83_000290"
     CDS             581..1375
                     /gene="panB"
                     /locus_tag="OQM83_000290"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCS1518649.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1387..2238
                     /gene="panC"
                     /locus_tag="OQM83_000291"
     CDS             1387..2238
                     /gene="panC"
                     /locus_tag="OQM83_000291"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCS1518650.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="OQM83_000292"
     CDS             complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="OQM83_000292"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCS1518651.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3700..4080
                     /gene="panD"
                     /locus_tag="OQM83_000293"
     CDS             3700..4080
                     /gene="panD"
                     /locus_tag="OQM83_000293"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCS1518652.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4084..5313)
                     /locus_tag="OQM83_000294"
     CDS             complement(4084..5313)
                     /locus_tag="OQM83_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCS1518653.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5377..5817)
                     /locus_tag="OQM83_000295"
     CDS             complement(5377..5817)
                     /locus_tag="OQM83_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCS1518654.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5922..6692)
                     /locus_tag="OQM83_000296"
     CDS             complement(5922..6692)
                     /locus_tag="OQM83_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1518655.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6689..7615)
                     /locus_tag="OQM83_000297"
     CDS             complement(6689..7615)
                     /locus_tag="OQM83_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1518656.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7724..8386
                     /gene="can"
                     /locus_tag="OQM83_000298"
     CDS             7724..8386
                     /gene="can"
                     /locus_tag="OQM83_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCS1518657.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="OQM83_000299"
     CDS             complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="OQM83_000299"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCS1518658.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            9169..11559
                     /gene="gcd"
                     /locus_tag="OQM83_000300"
     CDS             9169..11559
                     /gene="gcd"
                     /locus_tag="OQM83_000300"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCS1518659.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="OQM83_000301"
     CDS             complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="OQM83_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCS1518660.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13322..13669
                     /locus_tag="OQM83_000302"
     CDS             13322..13669
                     /locus_tag="OQM83_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCS1518661.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13775..14641
                     /gene="speE"
                     /locus_tag="OQM83_000303"
     CDS             13775..14641
                     /gene="speE"
                     /locus_tag="OQM83_000303"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCS1518662.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14657..15451
                     /gene="speD"
                     /locus_tag="OQM83_000304"
     CDS             14657..15451
                     /gene="speD"
                     /locus_tag="OQM83_000304"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCS1518663.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15489..15851)
                     /locus_tag="OQM83_000305"
     CDS             complement(15489..15851)
                     /locus_tag="OQM83_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCS1518664.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="OQM83_000306"
     CDS             complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="OQM83_000306"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCS1518665.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKIEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18978..20735
                     /locus_tag="OQM83_000307"
     CDS             18978..20735
                     /locus_tag="OQM83_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCS1518666.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="OQM83_000308"
     CDS             complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="OQM83_000308"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCS1518667.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="OQM83_000309"
     CDS             complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="OQM83_000309"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCS1518668.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="OQM83_000310"
     CDS             complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="OQM83_000310"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCS1518669.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="OQM83_000311"
     CDS             complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="OQM83_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCS1518670.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28591..29964
                     /gene="aroP"
                     /locus_tag="OQM83_000312"
     CDS             28591..29964
                     /gene="aroP"
                     /locus_tag="OQM83_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCS1518671.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="OQM83_000313"
     CDS             complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="OQM83_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCS1518672.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="OQM83_000314"
     CDS             complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="OQM83_000314"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCS1518673.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31497..32390
                     /gene="nadC"
                     /locus_tag="OQM83_000315"
     CDS             31497..32390
                     /gene="nadC"
                     /locus_tag="OQM83_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCS1518674.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32593..33033
                     /gene="ppdD"
                     /locus_tag="OQM83_000316"
     CDS             32593..33033
                     /gene="ppdD"
                     /locus_tag="OQM83_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCS1518675.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            33043..34429
                     /gene="gspE"
                     /locus_tag="OQM83_000317"
                     /pseudo
     CDS             33043..34429
                     /gene="gspE"
                     /locus_tag="OQM83_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            34419..35621
                     /gene="hofC"
                     /locus_tag="OQM83_000318"
                     /pseudo
     CDS             34419..35621
                     /gene="hofC"
                     /locus_tag="OQM83_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
     gene            complement(35656..36699)
                     /gene="guaC"
                     /locus_tag="OQM83_000319"
     CDS             complement(35656..36699)
                     /gene="guaC"
                     /locus_tag="OQM83_000319"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCS1518676.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(36855..36899)
                     /locus_tag="OQM83_000320"
     CDS             complement(36855..36899)
                     /locus_tag="OQM83_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1518677.1"
                     /translation="MIIKTLKMSARKRN"
     gene            36924..37544
                     /gene="coaE"
                     /locus_tag="OQM83_000321"
     CDS             36924..37544
                     /gene="coaE"
                     /locus_tag="OQM83_000321"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCS1518678.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37544..38287
                     /gene="zapD"
                     /locus_tag="OQM83_000322"
     CDS             37544..38287
                     /gene="zapD"
                     /locus_tag="OQM83_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCS1518679.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38297..38494
                     /gene="yacG"
                     /locus_tag="OQM83_000323"
     CDS             38297..38494
                     /gene="yacG"
                     /locus_tag="OQM83_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCS1518680.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38594..38983)
                     /gene="mutT"
                     /locus_tag="OQM83_000324"
     CDS             complement(38594..38983)
                     /gene="mutT"
                     /locus_tag="OQM83_000324"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCS1518681.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(39043..41748)
                     /gene="secA"
                     /locus_tag="OQM83_000325"
     CDS             complement(39043..41748)
                     /gene="secA"
                     /locus_tag="OQM83_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCS1518682.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41810..42322)
                     /gene="secM"
                     /locus_tag="OQM83_000326"
     CDS             complement(41810..42322)
                     /gene="secM"
                     /locus_tag="OQM83_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCS1518683.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42553..43470)
                     /gene="lpxC"
                     /locus_tag="OQM83_000327"
     CDS             complement(42553..43470)
                     /gene="lpxC"
                     /locus_tag="OQM83_000327"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCS1518684.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43571..44722)
                     /gene="ftsZ"
                     /locus_tag="OQM83_000328"
     CDS             complement(43571..44722)
                     /gene="ftsZ"
                     /locus_tag="OQM83_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCS1518685.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44783..46045)
                     /gene="ftsA"
                     /locus_tag="OQM83_000329"
     CDS             complement(44783..46045)
                     /gene="ftsA"
                     /locus_tag="OQM83_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCS1518686.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(46042..46872)
                     /gene="ftsQ"
                     /locus_tag="OQM83_000330"
     CDS             complement(46042..46872)
                     /gene="ftsQ"
                     /locus_tag="OQM83_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCS1518687.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46874..47794)
                     /gene="ddlB"
                     /locus_tag="OQM83_000331"
     CDS             complement(46874..47794)
                     /gene="ddlB"
                     /locus_tag="OQM83_000331"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCS1518688.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47787..49262)
                     /gene="murC"
                     /locus_tag="OQM83_000332"
     CDS             complement(47787..49262)
                     /gene="murC"
                     /locus_tag="OQM83_000332"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCS1518689.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49316..50383)
                     /gene="murG"
                     /locus_tag="OQM83_000333"
     CDS             complement(49316..50383)
                     /gene="murG"
                     /locus_tag="OQM83_000333"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCS1518690.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50380..51624)
                     /gene="ftsW"
                     /locus_tag="OQM83_000334"
     CDS             complement(50380..51624)
                     /gene="ftsW"
                     /locus_tag="OQM83_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCS1518691.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51624..52940)
                     /gene="murD"
                     /locus_tag="OQM83_000335"
     CDS             complement(51624..52940)
                     /gene="murD"
                     /locus_tag="OQM83_000335"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCS1518692.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52943..54025)
                     /gene="mraY"
                     /locus_tag="OQM83_000336"
     CDS             complement(52943..54025)
                     /gene="mraY"
                     /locus_tag="OQM83_000336"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCS1518693.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(54019..55377)
                     /gene="murF"
                     /locus_tag="OQM83_000337"
     CDS             complement(54019..55377)
                     /gene="murF"
                     /locus_tag="OQM83_000337"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCS1518694.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55374..56867)
                     /gene="murE"
                     /locus_tag="OQM83_000338"
     CDS             complement(55374..56867)
                     /gene="murE"
                     /locus_tag="OQM83_000338"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCS1518695.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(56854..58620)
                     /gene="ftsI"
                     /locus_tag="OQM83_000339"
     CDS             complement(56854..58620)
                     /gene="ftsI"
                     /locus_tag="OQM83_000339"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCS1518696.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58636..59001)
                     /gene="ftsL"
                     /locus_tag="OQM83_000340"
     CDS             complement(58636..59001)
                     /gene="ftsL"
                     /locus_tag="OQM83_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCS1518697.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58998..59939)
                     /gene="rsmH"
                     /locus_tag="OQM83_000341"
     CDS             complement(58998..59939)
                     /gene="rsmH"
                     /locus_tag="OQM83_000341"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCS1518698.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59941..60399)
                     /gene="mraZ"
                     /locus_tag="OQM83_000342"
     CDS             complement(59941..60399)
                     /gene="mraZ"
                     /locus_tag="OQM83_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCS1518699.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14596 a        16854 c        14998 g        14510 t
ORIGIN      
        1 agtggaactt ctgtgactgg ttctacggta aaaatgggtg aatatagtgc agaacaaatc
       61 agaaacggtg ccacaccggt tccttttgat atttcacttc aaaactgcgt tcgtgtgact
      121 aatattgaga caaaattagt ttcaacaaag gttggtactg aaaacgggca actccttggt
      181 aatactctca caggtaatga cgcagcaaaa ggagtcggtg tactcataga aggtttagca
      241 actagtaaaa atcctctaat gacattgaaa cctaatgatt caaattctgt ttataaagat
      301 tacgacccga gaggcaaaga cgatacaaca ggaggagttt acccggatca agatacaggt
      361 ataacatacc ctctccattt ccaggccacg ctacaacagg atggaactat accaatagaa
      421 gctggtgagt ttaaagccac cagtactttc caggtaacct acccttaata agtccaccgc
      481 accgccatca tctggcggtg cggtaattga taaatcattg cccccaccaa tgtgactgat
      541 aaaatgccag gcaacacgac atcatttatc aggacacgtt atgaaaccga ccaccattgc
      601 ttcacttcag aaatgtaagc aggataaaaa gcgtttcgcg accatcaccg cttacgacta
      661 cagcttcgcg aaactatttg ctgatgaagg acttaacgtc atgctggtag gcgactcgct
      721 gggcatgacg gttcagggtc acgactccac cctgccggtg accgttgccg atatcgccta
      781 ccacactgcc tccgtacgtc gtggtgcgcc aaactgcctg ctgttggctg acctgccgtt
      841 tatggcgtat gccacgccgg aacaagcctt tgaaaacgcc gcaacggtta tgcgtgccgg
      901 tgccaatatg gtcaaaattg agggcggtga gtggctggtc gaaaccgtaa aaatgctgac
      961 cgaacgtgcc gttcctgtat gtggtcactt aggtttaacc ccacagtcag tgaatatttt
     1021 cggtggctac aaagttcagg ggcgcggcga tgaagcgggc gatcaactgc tcagcgatgc
     1081 attagcctta gaagccgctg gggcacagct gctggtgctg gaatgcgtgc cgattgaact
     1141 ggcaaaacgt attaccgaag cactggcgat cccggttatt ggcattggcg caggcaacgt
     1201 cactgacggg cagatcctcg tgatgcacga cgccttcggc attaccggcg gtcacattcc
     1261 taaattcgct aaaaatttcc tcgccgaaac gggcgacatc cgcgcggctg tgcggcagta
     1321 tatggctgaa gtggagtccg gcgtttatcc gggcgaagaa cacagtttcc attaaggagt
     1381 cacgttgtgt taattatcga aaccctgccg ctgctgcgtc agcaaattcg ccgcctgcgt
     1441 atggaaggca agcgcgtggc gctggtgcct actatgggta atctgcacga tggccatatg
     1501 aagctggtcg acgaagccaa agcccgcgcc gatgtggtcg tcgtcagtat tttcgttaac
     1561 ccgatgcagt tcgaccgccc ggaagatctg gcacgttatc cacggacctt gcaggaggac
     1621 tgcgagaagc taaacaaacg taaagtggat ttagttttcg ccccttcggt aaaagagatc
     1681 tacccgaacg gtactgaaac ccatacttac gttgacgttc ccgggctttc gaccatgctg
     1741 gaaggcgcga gccgtccggg gcattttcgc ggcgtttcga ctatcgtcag caagctgttt
     1801 aacctggtcc agccggacat cgcctgcttc ggtgaaaagg actttcagca actggcgcta
     1861 atccgcaaaa tggttgccga tatgggcttc gatattgaaa ttgtcggcgt cccgattatg
     1921 cgcgccaaag atggccttgc gctcagttcc cgtaatggtt atctgacggc ggaacaacgc
     1981 aaaattgcgc ccggtctgta caaagtttta agttcaattg ccgacaaatt gcaggctggc
     2041 gagcgggatc ttgatgaaat tattgccatt gcagggcaag aactgaatga aaaaggcttc
     2101 cgcgccgatg atattcagat tcgcgatgcc gacacgttgc tggaagtctc tgaaaccagc
     2161 aaacgagctg taattctggt agccgcctgg cttggcgatg cccgcctgat cgacaacaaa
     2221 atcgtcgaac tggcgtaatg cttttactct aaataattca agttgcagga aggcggcaag
     2281 cgagtgacaa attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga
     2341 catggatgtc tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa
     2401 cgcagacgca gcacatgcaa tttaaagtat gacgagtata actggcgcta cggctgatgg
     2461 cgccaattat taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac
     2521 gcagacaact tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc
     2581 aacgtcttcc cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctttcctc
     2641 ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2701 catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2761 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2821 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2881 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2941 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gtgccagctc
     3001 cggcgagtaa aatatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     3061 ataaaacagt atcggcacca ccagcggcag cttatcgtga tccgcctcca gatgccggtg
     3121 catggcagca atagaataac gcatcatgcg aaagaccatt ttcttatccg gcttgctttg
     3181 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     3241 gtccgtgctg tgtcctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     3301 gttgaggtca caaagttctc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3361 cgtctccgcg tgcattaaaa attgtttaaa cactgcatca tgcggtgtgg tactcggtgc
     3421 atccatcttc cctgccttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc
     3481 catcgtcagc cacctcttta gtgtttaccg agcagcgttc agagaaattt caattggtta
     3541 cagccgttac atggcacgct tcggcacggt acgcaaactg ctgatgtaaa tgccgtcaga
     3601 tgagtagaca ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcat
     3661 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3721 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3781 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg
     3841 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3901 attatttctg ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc
     3961 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat
     4021 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     4081 ttgttatccc tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct
     4141 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     4201 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     4261 tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcggcg
     4321 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4381 atagccatcc acccgatgca aaaaatgggt atgtgactga aaatcaaaaa catcgcgaat
     4441 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4501 acgtttgatg cgcgaggtaa caacaaacgc cgtcgccttc atgccatatt gtttcaatac
     4561 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4621 cgcagggaga ttgatcttat tcttcacgta accttccagc tgcgccatgc tcagtgtcgc
     4681 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4741 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4801 cggaaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4861 acggacctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4921 caaattgtcc gccaataccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg
     4981 cgtatctttc caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc
     5041 gtcttcaact ttttgtcgcc cctgaaccgg ctcgagatgc cctttatcga taaacccttt
     5101 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     5161 tccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc agaccgcagc
     5221 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     5281 catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg tgaaacaacg
     5341 cttttattgt agcaaaagcg ctataagaat cgtgggttaa tactcataca aattatgttt
     5401 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5461 cagttcgaca atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat
     5521 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5581 taataatgaa gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc
     5641 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5701 taacccacgc cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag
     5761 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5821 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
     5881 caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5941 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     6001 ccaaaggtag tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg
     6061 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     6121 agtgagtaaa agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     6181 ctgatgtcat cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac
     6241 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     6301 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6361 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6421 ttacgctgga acttggcacc aaaaaatgat gacgcaacgt tggcgtaggc attggtgatc
     6481 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6541 ccaatacgcg aaccaatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6601 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6661 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6721 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6781 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt
     6841 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6901 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6961 aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg
     7021 cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca
     7081 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     7141 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     7201 cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt ttaagatatt
     7261 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     7321 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7381 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7441 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7501 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7561 gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7621 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7681 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7741 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7801 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7861 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7921 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7981 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     8041 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     8101 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     8161 gtgtgaactg aacgtcatgg aacaggttta taacctaggc cactccacca ttatgcaatc
     8221 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     8281 cttgctgcgt gatctggatg tcacagcgac caaccgcgaa acccttgagc aacgttaccg
     8341 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8401 atgcaacaca tccggcaact tcacacttac tcgtcccgca gaatcacttt gccgatatac
     8461 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8521 atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc
     8581 agcgtacaaa tcgccagcga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8641 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8701 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8761 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8821 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8881 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8941 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     9001 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     9061 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     9121 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     9181 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     9241 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     9301 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggtgcagtaa acgcgccgcg
     9361 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9421 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9481 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9541 gtggtggcac tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag
     9601 gaaatcaacg gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc
     9661 gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa
     9721 caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat
     9781 gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc
     9841 gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc
     9901 aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc
     9961 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
    10021 gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc gattaacgct
    10081 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
    10141 atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa
    10201 accatcgtga tggccggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg
    10261 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc cggcgcgaaa
    10321 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcctgg
    10381 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10441 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10501 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10561 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10621 gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgcaat
    10681 ggcgaactgg tggttccggc cccggaaaaa ccggtgccgc aaggtgctgc gaaaggcgat
    10741 tacgttactc cgacccagcc gttctctgaa ctgagcttcc gtccgaagaa agatttgagc
    10801 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10861 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg
    10921 ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10981 attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac
    11041 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    11101 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg tctgccgtgt
    11161 aaacagccag catggggtta tatctcggcg ctggatctga aaatcaatga agtggtgtgg
    11221 aagaaacgta ttggtacgcc gcaagacagt atgccgttcc cgatgcctgt accggtgccg
    11281 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    11341 atcgctgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11401 tggcagggcc gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggc
    11461 aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11521 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagagatcc
    11581 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11641 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11701 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11761 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcatg ccgtgaatat
    11821 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11881 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11941 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    12001 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    12061 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    12121 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    12181 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggcgcaatcg
    12241 ccatccccat ctggctgacc ggtagcgtca ccagatcaaa gggtttgttg tcgttaacct
    12301 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12361 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12421 aattgagcga gcgggcgtta cagccattga gcaaacgcag gcgcagccag ccacgcgggg
    12481 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12541 cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat tttttatcct
    12601 gaacaatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12661 cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc ccggtcttgc
    12721 catgttgatg tggatggaac cagcaggtag cggcaggttg atcaacgttc aacgtcaccg
    12781 tacgtttgcc gcctggcgga ataattccct gcgggccgcc gtcgacgtca cccggtactt
    12841 ccagcccgtg ccagtgcaac gtcgtctctt ccgccagttg gttatagata tcaaccgtta
    12901 ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg
    12961 tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac tgaatgcgat
    13021 tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga
    13081 ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa tattttaaga
    13141 aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt
    13201 gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa
    13261 tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc
    13321 aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgtct gcgcaaacag
    13381 ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtctt
    13441 tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc gcgcactggt
    13501 ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc
    13561 cctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaagtg
    13621 caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc
    13681 tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt
    13741 tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac agtggcatga
    13801 aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt atcatgaaaa
    13861 gaccgatcac caagatctga tcatttttga gaacgctgca tttggtcgcg taatggcgct
    13921 ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga tgatgaccca
    13981 tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg
    14041 tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat
    14101 cgatgcgggt atcgtgtcgt tctgccgtca gtatctgccc aaccataacg ccggtagcta
    14161 cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta atcaaaccag
    14221 ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagtct
    14281 gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt
    14341 cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagct atcgcaaact
    14401 cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct attacggcgg
    14461 tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat
    14521 tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcaatcca
    14581 tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac agccgtccta
    14641 aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag
    14701 tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc gcgacggtta
    14761 tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac
    14821 ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg aaccacaggg
    14881 tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca tcgacaaaac
    14941 agaacacccc ggcccactgc cagaaacggt cgttgcccat ctcgataaaa gtcatatttg
    15001 cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat
    15061 tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca
    15121 gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc gcgacattaa
    15181 cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga
    15241 tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata tcttccatac
    15301 caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga
    15361 tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat
    15421 ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc aggctccgcc
    15481 agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg cagaaaatcc
    15541 tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccttc ttccatttca
    15601 tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc catccacagg
    15661 gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct
    15721 tgttccactt catcaagcaa agccaggttt tctttcacct cttcattaaa ccagtgcccg
    15781 accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc gcgcagaaat
    15841 tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca ttgcgctatt
    15901 gatgtaatta ttgcagcctt accggagaaa aacagcggaa cggcgatggt ttagaaataa
    15961 aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt
    16021 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    16081 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    16141 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    16201 gcagaagcca ggaagacatt cgcgccagtc cccagacggt tcgggaagtt acgggtagag
    16261 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagagaacag
    16321 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttcagtc
    16381 aactgtgcgg cgtccatgcg ggttggcggt gccacccaca ggcgggtcgg caactggcct
    16441 ttgtgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttagt catgcaagaa
    16501 ccgataaaca cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcgtcc
    16561 gggtcgttcg gtgcacagag gattggctct ttaatatccg ccagatcgat gtcgatcact
    16621 gccgcgtatt cagcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16681 atgccctgaa tacgacgttc gagggtacga cgatcgccat aaccttccgc gatcatccac
    16741 ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt cagcttgata
    16801 gtacaaccag cggcagaacg ctcagcggac gcatcggtca gttcaaatgc ctgctcaatt
    16861 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16921 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc gtgtaccaga
    16981 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    17041 atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga acccgccggg
    17101 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    17161 agcagcatac ggttcagcca ggagtggatt acgccgtcac ccggacgcag cgacacacca
    17221 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    17281 tacgccgcgg tatggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    17341 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgtgaacc tacagaagtc
    17401 attttcggtt cgcagtacgc gcccggacga atacctttca caccgcaggc acgacctacc
    17461 attttctgag ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17521 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17581 atcagcggaa tacggccacc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17641 aaggtcgcca gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17701 tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag tgcacccgcg
    17761 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17821 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17881 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17941 tgttgcagag cttcgatttg cttgatcggg ccaacaacac ctggctggtc tggctcaata
    18001 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgcgaccac
    18061 gcatccggtg ccggagaaag gtcatcagtg ttggtttcgc cagtgacttt gaagacggta
    18121 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    18181 tgcattacct gcttcgcata ttcgttgcct gctttggctt tctcttctac gtcatagaag
    18241 ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc cagtttggca
    18301 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt acccagcagt
    18361 tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttagc gatagcagcc
    18421 aggaagcctg ctttgacata ggcggcttca tcgacgccag ggggaacacg gttggttaat
    18481 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg
    18541 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18601 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18661 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18721 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18781 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18841 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18901 cgcctcttta tactcggatt aacagcacct gcgggtggca gttcgccgac cattgcgatt
    18961 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcactaa
    19021 tttgcagtgc cgggctttgt gccgcctctg ccgggctata tataaaaagc cgcacagtgg
    19081 aagcgcctgt ggaaacgcaa tcgacacaac agactgcgcc tgacatcacc gcagttacgc
    19141 ttcctgcaac ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc gcattcagca
    19201 ctgcacaaat cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct ctgctttcac
    19261 aagtgttaat ggcatcaacc tatccggcaa acgttgctca agcagtgcaa tggtcgcacg
    19321 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19381 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19441 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19501 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19561 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19621 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19681 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19741 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19801 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19861 acgcactatt tagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19921 atgatgatta tcatcaccac gatggcggtc atcgtgacgg taatggctgg caacacaacg
    19981 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    20041 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    20101 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    20161 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    20221 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    20281 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    20341 tgacccagca acaaaaggac gccgctcgtc agcgttatca atcggcctct cctgagcagc
    20401 gccaggcggt tcgcgagaaa atgcagacta acccacagaa ccagcagcga agagaggcag
    20461 cgcgtgagcg tattcagtct gcaactcccg aacagcgcca ggtgtttaag gaaaaagtac
    20521 agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtata caatcggcat
    20581 cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca caacgtctaa
    20641 acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcagcagta cgcgaacgtc
    20701 tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa aaagcggcgt
    20761 ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aaaaattact tcttcttcgc
    20821 tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca ggcccacaga
    20881 ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat cacaacccat
    20941 ttcgattgcc agaccgattt cacccagcag ctcaccgccg ttagtaccga caatcgcacc
    21001 accgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt ctgcgcagtc
    21061 ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat agctgatgcc
    21121 tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt cggtataggc
    21181 gatagacggg ataactttcg gatcgaagta gtgtttttta ccggcgataa cttcagcggc
    21241 aacgtgacct tcgtgaacac ctttgtgtgc cagcatcggc tgaccaacga tatcgccgat
    21301 agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga aaccacggtc
    21361 gtccacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca cacgaccaat
    21421 cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc cttccatcgt
    21481 cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca gcatcaggtt
    21541 gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag ctgccgggat
    21601 aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt atacggtgcc
    21661 catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta cttctttcag
    21721 ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa acggcagttg
    21781 gatcgggcga gaacccgctg caatgatcgc attgtcgaag ttgatcacgg ttttgccgtt
    21841 ctcaccttca acttccaggg tgttagcccc ggtgaattta cccagaccgt tgaccacttt
    21901 gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga tcactttctc
    21961 tttccaagta cgaatcttgt caatatcggt tttcggttcg ccgaagacga taccgtgttc
    22021 agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt tagaagggat
    22081 acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga ttacggtttc
    22141 cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg ccccaagtac
    22201 cacgacctga gttttgattt cagtactcat catgacctct atatatttat ctccggcggt
    22261 catacccgtc gtctttcagg ccgcagcggc gttagcttca cgccctcacc ccagtcactt
    22321 acttatgtaa gctcctgggg attctggcgc ttgctacctt gctgcaccca gaaatccata
    22381 gggtatgtga agtaataccc taaccaccac cgggtcgttc tatccgtcgg tattttacaa
    22441 aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc taataacctc
    22501 aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt ttacttacat
    22561 caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac gggcaccatc
    22621 agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca gacgcggcac
    22681 gaactcttta ccattccaca ccggctccat cgcggacttg gaaacgccga ggatagccac
    22741 ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga tgctggagat
    22801 ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg ctttcttaga
    22861 aatagtcatc agctcgcgag acagctcgat gatgcctttc ttgttgacgt ctttgaatac
    22921 cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt atttcttcag
    22981 ggtcagacgc tgaccgtctt ccgacagcga actattgaag cgaggcatct gctcaagagc
    23041 tgcagcaacg gctttcatga tgaagacaac cggggtgagc ttcacatcca gcttacgttt
    23101 cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat cggttttgtc
    23161 gaagtgggta acatgcggga tcatcaccca gttacggctc aggttagcac cagaaatttt
    23221 ctggatacgg cccagttcca cttcttcgat ttcgccaaac ttgctgaagt ccaccttcgg
    23281 ccacggcagc atgccaggga taccaccgcc agtcgccgcc ggagctgctt ctgcacgttt
    23341 gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt tacgaccagt
    23401 gcccttcact ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga tcagcggagt
    23461 cgcgtgaacg taagcgtcgt tttcagcaaa ttcagatttg ccttcggctt tcgcagccgg
    23521 agctgctgcc ggggcttcag ctttcgctgc cggagccggc gctgccgctt cctgtttcgc
    23581 aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg agccagtttt
    23641 cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg gagccgggac
    23701 ctccatagaa gctttatcgc cttctacggt gataagcgat tgttcagcgg caactttgtc
    23761 gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat ccggaacgtt
    23821 aacttctttc acgccagccg ctgatgcagg ggccgctgcc ggagctgctt cctgtttagc
    23881 cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca gcgagccggt
    23941 agacactttg tcacccacgt tcactttgat ctctttcacg gtgccagcaa acggagccgg
    24001 aacttccata gaagctttat cgccttctac ggtgatcagc gactgttcag cttcaacttt
    24061 atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga tatccggaac
    24121 gttaacgtct tttgccgccg cagccgcggg tgctgctgcc ggagctgctt ctttcttctc
    24181 ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa tcataatcag
    24241 tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga tacccgcctg
    24301 cggagacgga acttccatag aggctttgtc gccttctacg gtgatcagcg actgttcggc
    24361 ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat cagccccgat
    24421 gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag acgcgggtta
    24481 actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt cttatcgatt
    24541 tcgccacgtt tagccagttc gcccagcgcc gcaaccacga cataagaagc atcaacttcg
    24601 aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt acccagtacg
    24661 cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt catatagtcg
    24721 gtagatgcca ctgccggagc gtcgttcatc acctgagcga tatacggaac gcgcggagtt
    24781 tccagcggat gaagcatgtt ccagcgttca caatcctgac catcacgcgc cagctcggtg
    24841 aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag gatctctgcc
    24901 gcttcacgga cgtgacgcag gatagaaccg gagcccagca gctgaacttt acctttgcta
    24961 ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc accttccggc
    25021 attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac gttctcttgt
    25081 ttttcaccgt acatacgctc cagaccgtca tgcatgatga cagcaacttc gtaagcgtaa
    25141 gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct gtgaccatct
    25201 tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc gatcaggaag
    25261 ccacgcgctt gctggtcgcc agccgcccag cacagatcgc cgatacgctg gaagccgaac
    25321 atcgagtaat agatgtagaa cggaatcatc ggcagattgt tggtgctgta agaggtcgcc
    25381 gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc cttcctgcag aatctgacct
    25441 ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcgggatgta ctgctgaccg
    25501 ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt acgcgcttcg
    25561 tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat tacgttcagg
    25621 gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag cagcgcgccg
    25681 aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg gcttggcaga
    25741 taaccgtgca gtttctgacg ctgtgcgtgc agataggtat gctcttcaga accttccggg
    25801 aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt gaaacggtcg
    25861 cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat gtttttacct
    25921 tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac tgtcgctttg
    25981 cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc gtgaccacca
    26041 cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc ggtttcagga
    26101 tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa ggtctggtag
    26161 tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc tttacgcagc
    26221 agttcatccc aacggctacc ccacatcact ttgataacgt tccagccagc accttcgaag
    26281 atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc aagacgctgc
    26341 aggttacagt tgataacgaa gaccaggtta tccagttttt cacgggtagc gatggtgatc
    26401 gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta aacggtctgt
    26461 ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc ctggtaaata
    26521 gcaccaatcg gccccagacc catagatacg gtcgggaact gccagaattc cggcatcagt
    26581 ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa gttatccagc
    26641 tgctcttgag tcagacgacc ttccaggaaa gcacgagcgt acacgcccgg ggagatgtgg
    26701 ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa gaagtggtta
    26761 aagcacacat cataaatggt tgcggaagac tggaaggacg ccatatggcc gcccagttcg
    26821 aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat agctgaacga
    26881 atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac ggggatggtg
    26941 ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt gcgggcttca
    27001 gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg gatgaccgat
    27061 tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg gaaacgttct
    27121 gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct gtcccattga
    27181 actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc ttttatatac
    27241 gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat tctttcgttg
    27301 ctccagacga cgcagagaac gctcacggcg gctctcttca cgacttctgt cgagcaaaat
    27361 ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg gcttaccggc
    27421 cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca tctcgcgacg
    27481 cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct ccatacacct
    27541 tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct gatactggag
    27601 tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta tggcgtggtg
    27661 gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt aataagcggc
    27721 gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgattcag gatggtcgga
    27781 gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga caaaagtgcc
    27841 gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg cctcacgcaa
    27901 ggagggacgg gagacgtcga actgttttgc cagttcgcgt tccggtggga gtttttcgcc
    27961 cgggcggaga gtgccttcga gaatcaaaaa ctccagttgc tgctcaatca catcggagag
    28021 ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac ttgccgaagt
    28081 caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt caccttatgt
    28141 ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt gacatatata
    28201 acgtttcaaa attgtaacta tgcacaaatg tagactttac gtaggaaagg gattttttaa
    28261 cgattattaa actataaaaa tccgaattga accgattcac ttaccaattt tttgattttt
    28321 aagtcaataa aaacgaattt aaattcattc tgcatattga gaggggttga ggctgagctt
    28381 tacaaacggt ttctttttaa gcaactcatc ttcaaccatg cataaagcgg gtgcattcgc
    28441 tgccgcatac cattattctt gatctgacgg aagtcttttt gtaacaattc aaacttcttt
    28501 gatgtaaaaa aattaataca acaaacggaa ttgcaaactt acacacgcat cactgcgtag
    28561 atcaaaaaaa caatcaccgc acgaggtttc atgatggaag gtcaacagca cggcgagcag
    28621 ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg cgcgataggg
    28681 acagggctat tcctgggtag cgcctccgta atacagtccg cagggccagg gattatcctg
    28741 ggttacgcca ttgctggttt tatcgccttt ctgatcatgc gtcagctggg tgaaatggtg
    28801 gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt ataaatactg gggcagtttt
    28861 gccggtttcg cctctggctg gaactactgg gtactgtacg ttttagttgc catggcagag
    28921 ctgactgccg tgggtaaata cattcagttc tggtatccgg aaatcccaac ctgggtttct
    28981 gccgccgtat tctttgtggt gattaacgcc atcaacctga ccaacgttaa agtgtttggc
    29041 gagatggagt tctggtttgc cattatcaaa gttattgcga tagtagcgat gatcatcttc
    29101 ggcggctggc tgctgttcag tggcaacggc ggcccgcagg cgagcgttag caacctgtgg
    29161 gatcaaggtg gtttcctgcc gcacggcttc accggactgg tgatgatgat ggcgattatc
    29221 atgttctcgt tcggtggtct ggaactggtg gggatcaccg cagcagaagc tgataacccg
    29281 gagcaaagta tcccgaaagc gactaaccag gttatctacc gcatcctgat tttctatatt
    29341 ggttcgttag ccgttctgct ctcactgatg ccgtggaccc gcgttaccgc cgataccagt
    29401 ccgtttgtgc tgatcttcca cgagttaggc gatacctttg tggcgaatgc gctgaacatc
    29461 gtggtactga ctgcggcgct ctccgtgtac aacagctgcg tatattgcaa cagccgtatg
    29521 ctgtttggtc tggcacaaca gggtaatgcg ccaaaagcgc tggcgtctgt cgataaacgc
    29581 ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta cagcattgtg cgtattgatt
    29641 aactatcttg ctccggaatc cgcatttggc ctgttaatgg cactggtggt atccgcactg
    29701 gtgatcaact gggcgatgat cagtctggcg catatgaagt tccgtcgcgc caagcaggaa
    29761 caaggcgtgg taactcgctt ccctgctctg ctttatccgc tgggtaactg gatctgcctg
    29821 ctgtttatgg cggcggtact ggtgattatg ctgatgaccc caggaatggc gatttcggta
    29881 tacctgatcc cggtatggct gatcgtgtta ggtatcggct atctgtttaa agagaaaacc
    29941 gccaaagccg taaaagcgca ttaatctctc tacgccctca cccatgcagg gtgagggcaa
    30001 taatctttac accaacgctc cgtaaatcgt cagtagcgca atcaccacca cgaccacgaa
    30061 cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc tccaccttat cgacatgcgg
    30121 ttcacgcgcc agagagaact gcgccagacg cgttaacacc tgatactgcg aagtatggaa
    30181 gtcacccagc gaagcaaacc aggccggtaa cgctttctcc ccatgaccga tcaaggcata
    30241 taccacaccc gcaagacgaa ccggcaccca atccagtaca tgaagcacgg catcaatgcc
    30301 ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc cagtattgcc atgcacgcaa
    30361 aaacgcatac cccatcagcg taacgggtcc ccaggttccc cccacaatca accagaacag
    30421 cggtgcaaga taaaaacgaa agttaatcca cagcaatgca ttttgcagct cacgcaaata
    30481 ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc cagccatcgt
    30541 ggcacgggca tggctatcat tacgtgaagc agctgtcaga taagcatgat aatgaagacg
    30601 aactttacct gcgccaatac acagcaaacc aatcagcagc cacaccagca gcgtgggaac
    30661 gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa gtcacgccca tcgcaataat
    30721 ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga acgcttcaag
    30781 acgatgatca agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa ttaacaccag
    30841 taaggttgta aatagcgtca tgttgtctcc ttgctgacca gcgcacgaaa ccgtgcccaa
    30901 tcaaatgcag gaccgggatc ggttttccga tccggcgcaa tatcacaatg gcccgtcatg
    30961 ttattagcga tatcgggata gcgatctatc agtgcccgcg taaccgccgc aagctgttga
    31021 tactgcgcat cggtatacgc cagagtatcg gtgccttcaa gctcaatccc aatagaaaaa
    31081 tcattgcagc gttcacgccc ctgatactga gagactcccg catgccaggc acgtttatcg
    31141 aaaggaacat actggactat ttcaccatca cggcgaatca aacagtgagc ggagacgcgc
    31201 aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat caatagttcc agtgaataat
    31261 gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc taatattgtg caccaccagc
    31321 agggtgggtg tttcgtcatc cgggcggcaa tcgtaatgtg gtgagggaac gcggcgcgcg
    31381 ccaaccagcc acccctgttc taacaacatg caggatctcc ttatatgtgg tgctaatacc
    31441 cggttcagag tagcatgttt ctaccttatg attcgttagc tatctggagt tttaacatgc
    31501 cgcctcgccg ctataaccct gacacccgac gtgacgagct gctggaacgc attaatctcg
    31561 atatccccgg cgcggtggcc caggcgctgc gggaagattt aggcggaaca gtcgatgcca
    31621 acaatgacat tacggcaaaa cttttaccgg aaaactctcg ctctcatgcc acggtgagca
    31681 cccgcgagaa tggcgtattt tgcggcaaac gctgggttga agaggtgttt attcaactgg
    31741 caggcgacga tgtcaccata atctggcatg tggatgacgg cgatgtcatc aacgccaatc
    31801 aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa cgcactgcgc
    31861 ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt acgccactat gtcgaattac
    31921 tggaaggcac caacacgcag ttgttggata cgcgcaaaac cttacctggc ctgcgttcag
    31981 ctctgaaata cgcggtactt tgcggcggcg gagcgaatca ccgtctgggg ctttctgatg
    32041 ccttcctgat caaagaaaat catattattg cctccggctc agtgcgccag gccgtcgaaa
    32101 aagcgtcctg gctgcacccg gatgcgccag tagaagtcga agtagagaat ctggaagaac
    32161 ttgatgaagc cctgaaagca ggagccgata tcatcatgct ggataacttc gaaacagaac
    32221 agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg tctggcaacg
    32281 tcactgacaa aacactgcgc gaatttgccg aaacgggcgt ggactttatc tccgtcggtg
    32341 cgctaactaa acacgtacaa gcactcgacc tttcaatgcg ttttcgctaa ttgctgacag
    32401 atttatccgc tccatcgcgg gcggataatc tccccttccc caactttctt cgtaacgcct
    32461 cgcaaatttc ttgtcttttg ctgcaacgaa ataaaaaacc tcggaagctg ctttcccgtt
    32521 tcgttttttg cccctcgctt gtgctgatgg cgtttgccaa agtagcgcca accaaatcaa
    32581 ggagcgaaac agatggacaa gcaacgcggt tttacactta tcgaactgat ggtggttatt
    32641 ggcatcattg ccattttaag cgccattggt attcccgctt atcaaaacta cctgcgcaaa
    32701 gctgcactca ccgacatgct acaaaccttt gtgccttacc gtacagccgt agagttgtgc
    32761 gcgctggaac atggtggatt agatacctgc gacggtggca gcaatggcat tccctcgcct
    32821 accaccaccc gctatgtttc agccatgagt gtggcaaaag gcgtggtgtc gctgaccggg
    32881 caagaaagtc tcaatggact aagcgtcgtc atgacaccgg gttgggataa cgcaaacggc
    32941 gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca gcgcattgca gcaagcctgc
    33001 gaagatgtct tccgctttga tgacgccaac taaggagcgg caatgaatat tccacagctc
    33061 accgccctgt gtctgcgtta tcagggagtc ttgctggatg ccagcgaaga ggtggttcat
    33121 gttgcggtag tcgatgcacc ttcgcatgag ttgctggacg cattgcattt cgctaccacc
    33181 aaacgtattg agatcacctg ctggacgcgc caacaaatgg aaggccacgc cagtcgcaca
    33241 caacagacat tgcccgtagc tgttcaggag aagcatcagc ccaaagcaga gttgctgact
    33301 cgaacgttac aatctgcgct ggaacaacgt gcgtctgata ttcatatcga accagcggac
    33361 aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc atcctttacc ggatgtttca
    33421 ccggatgccg gagtcgcatt aaccgccaga ttaaaagtgc tgggaaacct ggatattgcg
    33481 gaacatcgcc tgccgcagga cgggcaattc actgtcgaac tggcaggaaa cgccgtctca
    33541 tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg tggtattaag gttgttacag
    33601 caggtgaacc aggcactgga tgttaacacg ctgggaatgc agccgttaca actggcggac
    33661 tttgctcatg ccttgcaaca accacaggga ctggtgctgg taactggccc tacaggcagc
    33721 ggcaaaacgg tcacgcttta tagtgccctg caaaagctga ataccgctga cattaatatt
    33781 tgtagcgtcg aagatccggt tgagatcccc atagccggac taaaccagac gcaaatccat
    33841 ccgcgtgccg ggctcacctt tcagggcgtt ttgcgtgcgt tattgcgcca ggatcctgac
    33901 gtcatcatga tcggagagat ccgctatggc tttaacggca gaaattgcca ttaaagccgc
    33961 gcaaaccggt cacctggtgt tgtctaccct acacactaat tccacctgcg aaacgctggt
    34021 acgtttacag caaatgggag tcgcccgctg gatgctctca tcagcgctta cgctggtaat
    34081 agcccagcgt ctggtacgta aactttgccc acattgtcgc cagcagcaag gggagcccat
    34141 ccatattcca gtcaatgtat ggccgtcgcc gctgccccac tggcaggcac ccggttgtgt
    34201 acattgctac cacggttttt atggtcgtac ggccttattt gaagttctgc ccataacgcc
    34261 ggtcattcgt cagcttattt ccgctaatac cgacgttgaa tcgctggaaa cgcacgcacg
    34321 acaggcgggt atgcgtacgc tttttgaaaa cggctgcctg gccgtggagc aaggcttaac
    34381 cacctttgaa gagttaatcc gcgtactggg gatgccgcat ggcgagtaag caactctggc
    34441 gctggcatgg cataaccggc gacggcaatg cgcaagatgg gatgctatga gcagagagcc
    34501 gtgctttgct gctcatggca ctacagcaac agatggttac cccacttagc ctgaagcgaa
    34561 tcgccatcaa ttctgcgcag tggcgaggag ataaaagcgc ggaagtcatt catcaactgg
    34621 cgacgctact caaagccggg ttaacgcttt ctgaagggct ggcactgctg gcggaacagc
    34681 atcccagtaa gcaatggcaa gcgttgctgc aatcgctggc gcacgatctc gaacagggca
    34741 ttgctttttc caatgcctta ttaccctggt cagaggtatt tccgccactc tatcaggcga
    34801 tgatccgcac gggtgaactg accggtaagc tggatgaatg ctgctttgaa ctggcgcgtc
    34861 agcaaaaagc ccagcgtcag ttgaccgaca aagtgaaatc agcgttacgt tatcccatca
    34921 tcattttagc gatggcaatc atggtggttg tggcaatgct gcattttgtt ctgccggagt
    34981 ttgccgctat ctataagacc ttcaacaccc cactaccggc actaacgcag gggatcatga
    35041 cgctggcaga ctttagtggc gaatggagct ggctgctggt gttgttcggc tttctgctgg
    35101 cgatagccaa taagttgctg atgcgccgac cgacctggct tatagtgcgg cagaaattgc
    35161 tgttacgcat cccgattatg ggttcactga tgcggggaca aaaactcacg cagatcttta
    35221 cgattctggc gctgacacaa agtgcaggca ttactttttt gcagggcgta gagagcgtca
    35281 gagaaacaat gcgctgcccg tactgggtgc aacttctgac acaaatccag cacgatatca
    35341 gtaacggtca tcccatctgg ctggcgctaa aaaataccgg ggagtttagc ccgctctgtt
    35401 tgcaattagt gagaacagga gaggcatccg gctcgctgga cctcatgtta gacaacctcg
    35461 cccatcatca tcgggataac acaatggcgc tggcggataa cctcgcagcc ttactggaac
    35521 cgacgttgct gatcataacg ggaggaatta tcggtacgct ggtggtggcg atgtatctgc
    35581 caattttcca tttaggcgat gcgatgagtg ggatgggata acgctggcgt gttgctccac
    35641 accagcgttg gaagattaca ggttgttgaa gatgcggttt tcttgttcct gcacacggat
    35701 aaatgtggtg cgcttggtca gttctttcag gcgtgaagcc ccaacgtatg tacaagctga
    35761 acgcaggccg cccaaaatat cacgcgcggt attttcaacc gggcctcgca gcggcagctt
    35821 aacggtttta ccttctgctg cgcgatattc cgcaacgccg ccaacgtgac gtttcatcgc
    35881 agactcggag ctcatgccgt agaacagcat aaatttctcg ccgttctcct caacgatgcg
    35941 accgccgctc tcttcgtggc ccgccagcat gccgccaagc atgacgaaat cggcaccgcc
    36001 gccaaaggct tttgcgacat cgcccggcgt ggtgcagcca ccgtcgctga cgatcattcc
    36061 gcccagaccg tgcgccgcat cggcacattc gattaccgca gaaagctgcg gataaccgac
    36121 gcctgttttg acgcgagttg tacaaacaga acctgggcca atgccaactt taacgatatc
    36181 agcaccggag aggataagct cctcacacat ttcaccagtc actacgttgc cagcacaaat
    36241 ggttttggtc ggccacgctt cacgcgcttt cgcaacgaac tgcacgaagt gttcggaata
    36301 accattcgcc acatcaatac aaacgaagtt taatgccggg ttcaggtcga gaatctgttt
    36361 agttttttcg aaatccgcat cagacgtacc ggtagaaacc atcacatgtt tcagcacatc
    36421 agcggaagaa ttgttgataa acgcttgcca ctcttcgaca gaatagtgtt tatgcacagc
    36481 agtcaaaata tcaaaagaag ccagcgcaga ggccatagaa aatgtaccta cggtgtccat
    36541 atttgcggcg ataatcggca cgccggacca gctctgacct gaatgtttga aggtgaattg
    36601 acgttccagt tcaacatcgg aacggctttt aagagtggag cgtttagggc ggatgagaac
    36661 gtctttaaaa cctaacttca gatcttcttc aatacgcatg tgcggattcc tggggttaat
    36721 ggctataagg gtaagagcgc aactccagtg acgctatcat acgcactaat caatgcggcg
    36781 caagactgcg aaattgtctt tttttacgct acaatcccat aaatttacct ggtaaatagt
    36841 gagcgaacgt ttcatcaatt tcgctttcgt gcactcattt ttaacgtttt gataatcatg
    36901 atgaattccc gggaatataa attatgaggt atatagttgc cttaacggga ggcattggca
    36961 gtggcaagag taccgttgcc aatgcgtttg ctgatctcgg aattaacgtc attgatgccg
    37021 atattattgc gcgtcaggtg gttgaaccag gtgcacctgc gctacatgcc attgctgatc
    37081 actttggcgc taacatgatt gctgctgatg gaacattgca gcgccgggcc ttgcgcgagc
    37141 ggatcttcgc caacccggaa gagaaaaact ggcttaacgc cctgctgcat ccgctgattc
    37201 agcaagagac gcaacaccag atccagcaag ctacttcccc ctatgtactg tgggttgtgc
    37261 cattgctggt agaaaactca ctgtataaaa aagcgaatcg agtgctggtg gtggatgtca
    37321 gcccagaaac gcaacttaag cgcaccatgc agcgcgatga tgtaactcgc gagcatgtcg
    37381 aacaaatcct tgctgctcag gcaacgcgcg aagcccgcct tgccgtggca gatgacgtca
    37441 ttgataataa cggcgcaccg gatgctatcg catcggatgt tgcccgcctg cacgcacact
    37501 atttgcagct tgcgtcgcag tttgtctcac aggaaaaacc gtaatgcaga cccaggtcct
    37561 ttttgaacat ccactaaatg aaaaaatgcg tacatggctg cgcattgagt ttttgattca
    37621 gcaactcacc gttaatttac ccatcgttga ccacgctggc gcgctgcatt tcttccgtaa
    37681 tgtcagtgaa ttactggatg ttttcgagcg cggcgaagtc cgcactgagc tgttgaaaga
    37741 acttgaccgg cagcaacgta aactccagac ctggattggc gtgcctggcg tggaccagag
    37801 ccgtattgaa gcattaattc agcagttaaa agcggcgggg agcgtattaa tttccgcgcc
    37861 gcgtatcggg caatttctgc gtgaagatcg tttgattgct ctggtgcgtc agcgactgag
    37921 catcccaggc ggctgttgca gctttgattt acctacattg cacatttggc tgcatctacc
    37981 ccaggcgcag cgcgacagcc aggtagaaac ctggattgcc agcctgaacc cgctcaccca
    38041 ggcacttacc atggtgctgg atttaattcg ccagtcggcc cccttccgta aacaaaccag
    38101 tctgaatggt ttttatcagg ataacggtgg cgatgccgac ttgctgcgcc tgaatctgtc
    38161 gctcgattca cagctttatc cgcaaatttc cggacataag agccgttttg ccattcgttt
    38221 tatgccgctg gacactgaaa acggacaggt accggaacgt ctggatttcg aactggcctg
    38281 ttgctaagga gtaaaaatgt cagaaactat tacggtgaat tgcccaacct gcgggaaaac
    38341 ggtggtgtgg ggtgaaatca gcccgtttcg gccattttgc tccaaacgtt gtcagctgat
    38401 cgacctcgga gaatgggctg ctgaagaaaa acgaatcccc agcagtggcg atctttccga
    38461 aagcgatgac tggagcgaag agccaaagca gtgacatttg tctgatgccg cacgtaggcc
    38521 tgataagacg cggacagcgt cgcatcaggc atcttgtgca aatgtcggat gcggcgtgaa
    38581 cgccttatcc gacctacaga cgcttaagct tcgcaattac cggttcattg gctggcggaa
    38641 aatcatcggc attaagaccg actagcgaca tccactcacc gggttgccct tctttacccc
    38701 acggctcccc ttcccagctt tcgaccagcc agaaccacag agttatatgc ctgtccggga
    38761 attcatattc cagtttttca aatagcgaaa aatgttgtgg ggtaatcccg acttcttcct
    38821 gaagttcacg cactaccgcc tgttccggcg tttcgcccat ttcaatttta ccgccgggaa
    38881 actccagttt attcgccatg tgcgcatctg ctgcgcgacg cgttataaag atttcattgt
    38941 tctcgttgcg aataatacct accgcaattt gcagcttttt cattgtctta aacctataaa
    39001 aaaggcgcag aatcctgcgc cttttacttc aactgttagc ttttattgca ggcgaccatg
    39061 gcactgcttg tattttttac cagaaccgca cgggcaagga tcgttacgtc ctactttgcg
    39121 ctcaccggtt tgcgccgcca gtgcagctgc ggctgcagag tcgtcatcct gatggctaag
    39181 ctgctgcatt tgcgctaaac gctcggcttc catacgacgc tgttgttcca gctcctcaac
    39241 ctcttcaggc atacgtacct ggactttgct cagcgtgctg ataacttcat attttaacga
    39301 ctccagcatc gctgcaaaca tggagaacga ttcacgtttg tattcctgct tcggatcttt
    39361 ctgtgcatag ccacgcaggt ggataccctg acgcagatag tccatcgccg ctaggtgctc
    39421 tttccacaga gagtcgagag tttgcagcat gacgcctttt tcgaagtgac gcatcatctc
    39481 agcaccaacc acttcttctt tacgctgata cacttcgatg gactgcgcca gaattcgctc
    39541 acgcagcgtc tcttcatgca gttctggttc tttatccagc cactcggcaa ttggcaaatc
    39601 gaggtcgaaa tcgttcttca gacgttcctg cagccccgga atatcccaca tttcttccag
    39661 tgactgtggc ggaatgtagg catcaatggt cgctttgaac acatcttcac gaatgctgtt
    39721 gatggtttcg ctcacatcgc tgacatccag cagttcgtta cgctgggagt aaatggcgcg
    39781 acgctgatcg ttagccacgt catcatattc cagcagttgc ttacgaatgt cgaagttacg
    39841 gctttcaact ttacgctggg cgttggcaat cgctttagtc acccacgggt gttcaatggc
    39901 ttcgcctggc ttcataccca gtttacgcat catgccggat actcggtcgg aagcaaaaat
    39961 acgcatcagc gcatcttcca tcgacaggta gaaacgggaa gaaccagcat ccccctgacg
    40021 accagaacga ccgcgcaact ggttatcgat acgacgggat tcgtgacgct cggtaccgat
    40081 gatatgcagg ccacctgctt ccagtaccgc atcgtgacgt acctgccagt cggctttaat
    40141 tttttcaatt tgctctgcgg tcggattttc cagcgcggca acttctgcct gccagctacc
    40201 accgagcaca atatctgtac cacgacccgc catattggtc gcgatagtca ccgcagccgg
    40261 ataacctgcc tgagcaacaa tcgccgcttc gttggcgtgg aatttggcgt tcaggacgtt
    40321 gtgcttaata ccggctttgg tcagttcgtt tgacaccagc tccgattttt cgatggagat
    40381 agtacccacc agcaccggct ggcctttcgc agtacgttct ttgatatctt caatgatcgc
    40441 ctgaattttt tccgcttcag tcatgtagac caggtccggc agatctttac gaatcattgg
    40501 acggttggtc ggaacaacga cggtatccag cttgtagatg gagctaaatt cgaaagcttc
    40561 ggtatcagca gtaccggtca tccccgccag tttttcatac agacggaagt agttctggaa
    40621 ggtgatcgaa gccagcgttt ggttttcgtt ctggatctgc acaccttctt tcgcttccac
    40681 agcctggtgc agaccatcgg accagcgacg gccctgcatg gtacgaccgg tgtgttcgtc
    40741 aacgatgata acttcaccat ctttaacgat gtagtcgacg tcgcgggtaa acagcgcatg
    40801 agcgcgcagc gccgccgtta cgtggtgcat cagcatgatg ttggccggag agtacagaga
    40861 ctccccttca tccatgatgc cctctttcac cagcagttct tcaatcagca ccagaccacg
    40921 ttcggtcagg ttcacctggc gagatttttc gtccaccgag aagtggcctt cgccctggaa
    40981 ggtttcggag tcttcttttt cctgacggat caggtgcgga ataattttat tcacgcgttt
    41041 atacatttcc gagctgtctt ctgccgggcc ggaaatgatc agcggtgtac gcgcttcatc
    41101 gatcaggatg gagtccactt cgtccaccag cgcatagtgc agtttacgct gtacacgttc
    41161 ttcagggctg aacgccatat tgtcgcgcag gtagtcaaag ccgtattcgt tgttcgtacc
    41221 gtaagtgatg tcagctgcgt aagcttcgcg ctttgccggt gctggcatgc ccggcaggtt
    41281 gataccgaca gtcaggccaa ggaattcaaa cagcggacgg ttgttttcgg cgtcacgttg
    41341 cgccaggtag tcgttgacgg taactacgtg cacgccttta ccggttagtg cgttcaggta
    41401 agcaggcagc gttgcggtca gggttttacc ttcaccggta cgcatttcgg cgatgcagcg
    41461 ttcgttaaga accataccgc cgagtaactg aacgtcgaag tgacgcatac caaagacgcg
    41521 cttacttgcc tcacgtacca cggcgaaagc ttccgggatc agattttcca gcacttcgcc
    41581 tttttccaga cgtgcacgaa actctgcggt tttccctttc agttcttcgt cggagagttt
    41641 ttccatctcc ggttccatgg cattgatgat gttgaccact ttgcgcatcc ggcgcagggt
    41701 gcgatcgtta cgactaccga aaactttagt taacaatttg attagcataa taaaatctca
    41761 aacgccccgc gttgcggagt taataaaatg aagtaaaggt ttattgttgt taggtgaggc
    41821 gttgagggcc agcacggatg ccttgcgcct ggcttatcca gacgggcgtg ctgaattttg
    41881 cttgtggggt aaaatgcgca taatcaatgc gataaccctt ttcagacggc gtgccttcct
    41941 gggtcagcag cgcgctgagc gtatccagta atgcaagatg ttgcgcctga agaggcaaag
    42001 actcttcagc aacgggcagt gtttgcggtg ccattgcgaa agaaagatga cggattaccg
    42061 tgcgaatggc atgttgatgc cagtaatcaa cggaatagtt cgaattcggg cggcgtgtgt
    42121 tcgcttccag caaggccaat tgaccaaagt taactttggc tgaaggctcg tggttgcggg
    42181 ttgtcgcttt tgcgggcgcg tttggttcgg cggcgttgct gagcgcaggc aaacctaaac
    42241 tcgccgcaac catccctaat aagagatgcg gccagaagta gcgtttacca aactgtcgcc
    42301 agcgcgtcag tattccactc acgttattgc catcccgatc gtattcaaac gtatttagcg
    42361 cacaaatctt atcattaaag cctgcagtcc acagcacgaa tgaagataag ttgtgcggaa
    42421 aagtgcttaa gaaaacagcg ttcgcaccgt tttttatagc tcttataaaa ttcaaaaaaa
    42481 aatgactggt tcacctggcc ggagagagtg ccagatttgc cagtcgaatt ttatacgaca
    42541 gtataaatgt cgttatgcca gtacagctga aggcgctttg aaggccaacg gcagttctgc
    42601 gtcgtcctgg aaggtcacat attcccaggc ttcctgtttc gccaggacag cctgcagcag
    42661 tttgttattc agtgcatgac cggatttata agcggtaaat gcaccaataa tattgtgacc
    42721 acacatgaac aagtcaccga tcgcatcgag cattttgtga cgcacaaatt cgtcttcaaa
    42781 acgcaggccg tcttcgttca gtacgcgata atcgtcaaca acgatggcac aatcgaagct
    42841 gccgcccagg cacaaaccac gggactgcag atattcgata tcacgcatga aaccgaacgt
    42901 acgcgcacgg ctgatctggc gcataaacgc atcagcggag aagttcatcg catagcgctg
    42961 gttgctggaa tcaatagccg gatggttaaa atcgatggtg aaatccagcg aaaaaccatt
    43021 gtacggctta aattcagccc acttatcgcc atcttcgaca cgaacagtct ctttgatgcg
    43081 aacaaatttt ttggcgcagt tcaactcgtc gataccggcg tcaagcagca ggtatacaaa
    43141 cggagcggcg ctgccgtcca tgatcgggat ttccggcgcg ttaacttcga taacaatgtt
    43201 atcgatgccc aggcccgcga gagcagcatt gaggtgctct acggttgaaa tccgtacatc
    43261 atgctcgttg accagacacg tacagagcat ggtatcacgc acagatttgg catcggccgg
    43321 gaaatctacc ggtggattca agtcggtgcg acgatagatg accccggtgt tggccggcgc
    43381 agggcgtaac gtcagagtga ctttcttgcc ggtatgtaaa ccgacacccg tcgcctgaac
    43441 gatacgttta agtgtccttt gtttgatcat cgtattatct cgccaaatta cctatccaac
    43501 cgaagtgtac tatacattcg gcgggccagt ttagcacaaa gagcctcgaa acccaaattc
    43561 cagtcaattc ttaatcagct tgcttacgca ggaatgctgg gatatccaga taatccggct
    43621 ctttcgcagt ttgcggcgca ttgtcattca cgactttagc aaccggcttc tgctcctggg
    43681 tcagcggagc catcccatgc tgctggtagc gatccatcac tggctgctga acctgcttat
    43741 tggtcaccag agtgatttca ggacgtttgt ccatgccgat acctgtcgca acaacggtta
    43801 cgcgcagctc gtcattcata tccgggtcaa gagaagtacc gataaccaca gtcgcgttgt
    43861 cggaagcaaa tgcacggatg gtgttaccta ccgtttcgaa ctcatccaga cgcaggtcga
    43921 agcccgccgt gatgttaacc agcacgccgc gcgcgccaga caggtcgata tcttccagca
    43981 gcggagaaga gatagccatt tcagcagctt cttccgcacg gtcttcaccg ctcgccacgc
    44041 cagaacccat cattgcgtag cccatctcag acattacggt gcgtacgtct gcaaagtcca
    44101 cgttcatcaa acccggacga gtaatcagtt cagcgatacc ttgcacagcg cctttcagta
    44161 catcgttcgc tgcgccaaac gcatccagca gggagatacc gcggcccaga actttcagca
    44221 gtttgtcgtt cgggatagtg atcagagagt ccacatgctt ggacagttca gtgatcccct
    44281 gctccgcgaa tgccatacgc ttcttgcctt caaagttgaa aggcttagtg acgacagcaa
    44341 cggtcaggat acccaaatct tttgccactt cagcgacgac tggtgctgca cctgtaccgg
    44401 taccaccacc catacccgca gcaataaaga ccatgtctgc accttccagc gccgcacgca
    44461 atgcatcgcg atcctcatca gccgcattgc gaccaacttc tggattagcg ccagcgccca
    44521 gtcctttggt gataccgcta ccgatttgaa tcgtctgtcc aaccgctgtt ttacgcagcg
    44581 cttgtgcatc ggtatttacc gcgaagaatt caacaccttc aatacgctcg cgcaccatgt
    44641 gttcaacagc attaccgccg ccgccgccga cgccgatgac tttaatcacc gcgtcattgg
    44701 taagttccat tggttcaaac atagtttctc tccgatttgt gcctgtcgcc tgaggccgta
    44761 atcatcgtcg gcctcataaa aattaaaact cttttcgcag ccaactattg agtcgcttga
    44821 tccacgagcc aactgatgct gtaacacgtt tttctacttc agcttcaccg ttaagatgtg
    44881 actctttccc atagtgaagc aatcccaccg ccgtcgaata atacggctcc tgagcataat
    44941 ccgttaaacc ggtaatgttc agcggcgcgc cgatacgcac ttgcgtatga aacacgcgct
    45001 gagcacaggc tgcaagacct tcgatctgcg ctgcgccacc ggttaataca atgcctgccg
    45061 ccaggtgatg tttaacccct tgttggcgaa gcttttcctg caactgcaat atctcttcgt
    45121 tgaccaggtt gagcagctcg gtatagcgcg gctcgatcac ctctgccagg gtctgacgtt
    45181 gcagactccg tggcggacga ccgcctacgc tcggcacttc cacgctctca tcttttccaa
    45241 cgatggaacc cagcgcacaa ccgtggcgaa ctttaatcgc ttcggcgtcg cttggcggcg
    45301 tgccaaaggc gtaagcgata tcactggtca cgacattgcc agcataagga attaccttag
    45361 tgtggcgcaa tgccccaccg gtataaacgg cgatatccat tgtaccacca ccgatatcga
    45421 cgacgcagac acccagttca cgttcatctt ccgtcaatac cgaataactt gatgccagtc
    45481 cggcaaatat cagttggtca actttcagcc cacaacgttc aaccgctttg acgatgtttt
    45541 tcgccatatc gttgtgacat gtgatcaggt gcacttttgc ctgcatccgc acgcccgaaa
    45601 gtcctaccgg attcttgatc ccttcctgat agtcaatcgc atactcttgc gggatcacat
    45661 gcagcacacg atgttcatcg cgcacacgca ccgatttcgc ggtatggacg acgttttcca
    45721 catcttcttg cgtcacttct tcttcagaaa taggcaccat accaatttca ttctggcagc
    45781 tgatgtgctt accagaaagc gccagatata ccgaagagat ctgacaatct gccatcaatt
    45841 ctgcctggtc aatggcgcgt tgtacgcact tgaccacgga ttcgaggtcg ttcaccccgc
    45901 ctttatccat accacgtgac gggcagctgc ccacgccaat gatattgacc ataccgtcgg
    45961 gcagaacttc ccctactaaa gcggcaacct tcgcggtacc aatctccagt cctactacca
    46021 gttttctgtc cgtcgccttg atcattgttg ttctgcctgt gcctgatttt gttgctgagt
    46081 agattcctct ggcggcaagg gcgcccagcc tactgccgct ccagagtcat aacgcaaatc
    46141 aacgtagcta atccgtttgc catcggtttg cgcctgctgc tgtaaaaccg gataaagttc
    46201 tacaaagcga gccaaacgtt tcatcgtatc gccccggcca agattgagct taatatcgtt
    46261 attcagcgtc aactgccagg aacgccgcgc ggtcatcgcc gcttccttca gagtaaatct
    46321 gtcctttgcc agcatctgcc ccatttcgcg atagccctgc aacacttcat tggcgctgcc
    46381 ttccgggcca tacagcattg gaagcacctg cttgctggtg cgatctggcg gcacgctgaa
    46441 ggtgtttcct tccgcgtcta ccatatgttg atcattccac cgcgcaatcg gcacatattc
    46501 aaccagatga atcttcaatt catcaggcca ctgctttctg acgctcacct gcttaatcca
    46561 cggcaggcgt tgttctattt gcgtctggat gatgttgaca tcctgggtca taaaggtacc
    46621 cggctcaccc aatgccagga tcgactgccg gatatcgtca ttacgcgtgt aatggcgttc
    46681 accggtcaac accagctttg agagcggcag gcgttgcgca tcttccatcc agcccaacac
    46741 gacccagccg ctcaccaaca ctgtcgttaa aacggtcagc aggaaaagga tccccgccag
    46801 acgcgttcca ttattgcggc gagaagaaac ctcttcttcg ctatttcgcg tgttcagagc
    46861 agcctgcgac atattagtcc gccagttcca gaattcgtac taccaactgc gagaagctca
    46921 tacctgcctg acgtgccgcc atcggcacca ggctgtggct ggtcataccc ggtgaggtat
    46981 tggcttccag cagataaaac tgtccatcgc tgtccagcat aacgtcaata cgtccccatc
    47041 ctttgcaacc taacgtcgtc catgctttca gcactaatgc ctgcaaattg gcctcttgtg
    47101 acgcttccag acctgcgggg cagaaatact gtgtctcatc agagagatac ttcgcctcat
    47161 aatcatagaa ggttccggac ggttgaatac gtattgacgg taaaatttct tcaccgagta
    47221 tcgcaaccgt gaactccggc ccacttagcc atttttcaat caatacttct tcatcgtgct
    47281 gaaatgccaa tcttaatgca tcttgtagag cattttctgc tactactttt gacattccca
    47341 cactggaacc ttcgcggctc ggcttaacga taaccggcaa acccagagca gaaatttctg
    47401 ctaactgctt atcgctcagg cctttttcaa actctgcgcg ggttaacgct acccacggcg
    47461 cgaccggtaa accggcacct tgccacagca gtttgctgcg tagtttatcc attgaaagcg
    47521 cagatgccat cactccgctg ccggtataag gcaagcccat cagctcaagc atgccctgca
    47581 gcgtaccatc ttcaccgccg cgaccgtgta gcgcgataaa cactttctga aaacccatcg
    47641 acttcagttg cgtcacgtcg acttctttcg ggtcgacagg atacgcatca ataccgcctt
    47701 cacgcagccc ggctaacacc gctgcgccag aattcagaga aacttcccgc tcagcggaag
    47761 tcccgcccaa caggaccgca attttatcag tcatgttgtt cttcctccgg agtttgcggc
    47821 ttcagtttga tttcagctaa agaacgggca atttttccga tattaccagc cccctgaacg
    47881 agaatcaggt cgttaccgat taataccggt gccagcatct cggctacctg cgcagggtcc
    47941 ggcaccagaa tgggatcaat tttcccacgt ccacgaattg tgcgacacag cgaacggctg
    48001 tccgctcccg gaattggcgc ttcgccagcc ggatacactt ccagcatcaa cagggtatca
    48061 acctgcgtca gcacattggc gaaatcatca tacaggtcgc gcgtacgggt aaaacggtgc
    48121 ggctgaaaca gcattactag gtttttatcc ggccagcctg cgcgcgccgc tttaatggtg
    48181 gcgtcaactt ccgtcgggtg gtggccgtag tcatcgacca gcattgccgt accgctttta
    48241 ccattcactg gctccagcgg gaattcaccg aggaaatcaa aacggcgacc agtcccctgg
    48301 aagctctcca gcgcacgcaa aatagcctcg tcgtcaattc cctcttccgt agcaaccgca
    48361 accgcagctg cggcgttcag cgcgttatga cggcctggcg cattcagggt gacgcgcatc
    48421 ggctctttgt cctggcgcag cagcgtaaag tgcccctgcg ggccaatctg ctgataatct
    48481 tctacacgca cgtcggcatc ttcgctgaag ccgtaagtcg tggtctgacg tcccacacgc
    48541 ggtaacaatt cgcggatcac cggatcatca acacacatca ccgcacgacc gtaaaacggc
    48601 aggttgtgca gaaaattaat aaaagtctgt tttaaattct caaagtcgcc ctggtaggta
    48661 tccatgtggt cggcttcgat attggtgaca atcgccacca tcggttgcag atgcaggaac
    48721 gatgcatcac tctcatctgc ttcggcaatc aggtaccgac catgccccaa acgcgcatga
    48781 acccccgccg cttttaccag cccgccgtta acgaaggttg ggtcgagccc cgcttctgcg
    48841 tagatgctgg aaaccatcgc ggtggttgtc gttttgccgt gcgttcctgc aatggcgatg
    48901 ccatgacgaa aacgcattaa ctcagccagc atttcggcac gacggatcac cggaatacgc
    48961 gcttcatgag ctgcgacaat ttccgggtta tcggcagaaa tggcgctgga aacaacgacc
    49021 acgctggcat cacgtacgtt ttccgggcga tggttgaaat aaatcgtcgc tcccagattc
    49081 attaactgct gcgtgaccgg atttggcgct aaatcggaac cactgatctg ataaccttca
    49141 ttggccagaa cttcggcaat accgcccata ccggcaccac cgatgccgac aaaatgtatg
    49201 tgccgaacgc gacgcatttc gggcacgatg gaacgcagtt ttgccaattg ttgtgtattc
    49261 attctttacg ccattaactt cttaaattca tacgatgcaa aaggcatcgc tacaattacg
    49321 cccgggcagc ccggctcact tcatttgcca ctcgctcggt ggcatccgga atggatgcag
    49381 cgcgggcgcg ttctgccatg gttaataagg tttctcgcga ccacccggcc agggtgttgg
    49441 cgacagcatc cacgctaagc tgtggctgct cgataatttt ggctgcgccc gctttttcca
    49501 gcggtagcgc attccagtat tgctggcggt ctttatgttg aaacggcaca aacaacgccg
    49561 gtagtcctgc cgcggcgatt tcactcaccg ttaaagcacc ggagcggcaa acgacgacat
    49621 ccgcccacgc atacgccgcc gccatatcat caataaattc cgtcacttta tgctgcggtt
    49681 gccccgcttc ggcatacgcc tgttcaacgg attgttgcga acctttaccg ctctgatgcc
    49741 agatagtgac tgaatcaccc agtttcgcag caacctgcgg cattgtctgg ttaagaatgc
    49801 gtgcgccctg ggaaccaccc actaccagca cacgaaccgg accttcacgt ccagccaaac
    49861 gttgctgcgg caacggcagc gccagcacat cggtacgcac cgggttaccc accacttccg
    49921 cattagggaa agcgcctgga aacgcctgca tcactttggt ggcaatcttc gccagccatt
    49981 tattggttaa gcccgcaata ccgttttgtt catgaagtac aaccggaatg cctaacgacc
    50041 acgcggccag accacctgga cctgatacat agccgcccat accgagtacc acgtcaggtt
    50101 tgtacgcttt cataatcgcc cgcgcctgac gccaggcgtt gaagatacgc agcggcgcag
    50161 ctatcagtgc ttttatacct tttccacgca gaccagagat acgaatgaaa tcaatttcga
    50221 tgccatgttt tggcactaag tccgcttcca tacggtcggc agtccccagc cagcgaactt
    50281 gccaaccctg agccattaga tggtgcgcaa ccgccagtcc cgggaataca tgtccaccgg
    50341 ttccgcctgc catcaccatt aatcgctttc cttgaccact catcgtgaac ctcgtacaaa
    50401 cgcctgcgct ttctccagac gcgtttcata atcaatacgc aacagcatca tgatggctgt
    50461 cgacataatc agtaagctcg aaccaccgta actgatcagc ggcaatgtca gacctttggt
    50521 cggtaacatc cccgccgccg cgcctacgtt aaccagcgcc tggaagctaa accagatgcc
    50581 aatagaacag gcgagaaaac cggaaaaacg gtggtcaatt tctaacgctt tgcggccaat
    50641 cgacatcgcg cgaaaagcga cgaagaatac cattaaaagt gccagcacca caccgacata
    50701 ccccagttct tcgccgataa tggcgaaaat aaagtcagtg tgcgcttccg gcagatactc
    50761 cagtttttgt accgagttac ctaaaccttg tccccaaagt tcgccgcgac caaacgccat
    50821 cagcgattgc gttaactgat agccgctgcc aaagggatct tcccacgggt tccagaatgc
    50881 ggtaacacgg cggatacggt acggttcggc gagtatcagc aacacaaccg ctgaaatgcc
    50941 cataccgata atggcaatga actgccacaa tttcgctccc gccaggaaca acatcgccag
    51001 cgtagtcaca aacaacacca ccaccgtacc aaggtctggc tgtgccagca gtaacactgc
    51061 caacaccaga atcacgccca tcggtttcag gaagccgcgc aggttattac gtacttcgtc
    51121 gcctttacgc accagatagt tggcgatata acaaaacagc gacagttttg tcagctccgc
    51181 aggctggata cgcagcaaac cgagatcgat ccaacgcgat gcccctttaa ccgagctacc
    51241 cactaccagc acgatcatca gcaggatgat agatccgagc agcatcgtgg cactgtagcg
    51301 ttgccagaac tccatcggca gacgcagcgt aatgatcgcc agaatgaatg ccaggatcag
    51361 atagacgcca tcacgcttcg cgaagaagaa cggatcgttg gttaagcgtt gccctatggg
    51421 cattgacgcc gaggtcacca tgataaagcc aatcgccgca aggccgaagg tcagccacag
    51481 taaggtgcga tcgtacatga tcaggctgtc ggtatctttt tcccgcgagc ccatcaccca
    51541 gccttttagc gccgtggaga tccagaccag gatactgaat cctggcaggc gcggcatttt
    51601 caggcgaggg agagataaac gcatcaacct aactccttcg ccagacgggc aaactcattg
    51661 cctcgttgtt caaagttctt gaactgatca aggctggcac aggccgggga gagcagaacc
    51721 atatcgccca gctgaacacg cggagccagc aagcgcatcg cttgttccat ggtttcggtt
    51781 tgttctgcca cttccgggcg tagcgccgcc agctgcgcac cgtcacgacc aaaacagtac
    51841 aaacgcacgt tgtcgccatt cagatagcgc gccagtggac tgaagtccgc cgatttacca
    51901 tcgccgccca gcaacaaatg cagcgtgccg tctacctgca agccattcaa cgccgcttcc
    51961 gtactgccga cgttagtggc tttcgaatcg ttaatccagc gtacgccatt gtgctccagc
    52021 accacttcaa agcgatgcgg cagaccggtg aaggtggtta acgctttcag gctgctggcg
    52081 cgcggcaaac cagctgcgtc tgccagtgcc agtgctgcca gcgcattggt gtagttatgc
    52141 tgcccggaaa gtttcatctc tttcacattc agcactttct cgcctttaac ccgcagccag
    52201 gtttcgccct gctgatgatt caggtgatag tcacccatgt tgacgccaaa gctgacgcag
    52261 cgttcatcag caccgcgaat cggcattgtt aaggcatcat cagcattgac gacacaaact
    52321 ttcgcatttt cgtaaatacg caattttgcc gcacggtatt gctgcaaacc aaacggatag
    52381 cggtccatat gatcttcagt cacgttcaga atggtcgccg ccaccgcctg taagctggaa
    52441 gtggtttcca gctggaagct cgacagttcc agcacgtaca gttcgcactc agcatcaagc
    52501 agcatcaacg ctggcaggcc aatattgcca cccacaccaa cgttaacccc cgccgctttc
    52561 gccatttcac ccactagcgt ggtgaccgtg cttttgccgt tagaaccggt aatcgccaca
    52621 atcggtgctt gtgcttcgcg acagaacagc tcgatatcgc caacgatttc gattccggca
    52681 tcagcggcag cgcttaagga tggatgcgcc tgtgcaatac cgggactggc gacaatcaga
    52741 tctgccgcca tcagccattc atcattcaga ctgcccgtgt ggcgttctac ggcttcgggt
    52801 aatttatcca ggccaggcgg tgtcatacgc gtatccataa cgcgcggcgt cacaccgcga
    52861 gcgaggaaaa agtccacgca ggaaagcccg gtgaggccca ggccgataat gacgacattt
    52921 ttaccctgat aatcagccat gattaacgta ccttcagcgt tgccagacca atcagaacca
    52981 gcatcagcga aataatccag aaacgcacaa tgacgcgcgg ttccggccag cctttcagtt
    53041 catagtggtg atgaatcggt gccatgcgga aaatacgttg tccgcgcagt ttaaaggagc
    53101 cgacctgcag gatgacagaa agcgtttcta ccacgaacac gccccccata atcaccagca
    53161 ggaattcctg acgtagcagt acggcgataa tgcctaacgc accacctaac gccagcgaac
    53221 ctacatcgcc cataaagacc tgcgccggat aggtgttaaa ccacaggaag cccagtcctg
    53281 ccccgactat cgccgtgcag acaatgacca gttcccctgc gtgtcgcaga tacggtatat
    53341 gcaagtagct ggcaaagttc atattgccgg tcgcccacgc caccagcgca aaaccaccgg
    53401 cgacaaatac ggtcggcata attgccaggc cgtcgagacc atcggtcagg tttaccgcgt
    53461 tgccagtacc cacaatgacg aagtaagcca gcagaatgta gaacagcccc agctgcggca
    53521 tcacatcttt aaagaatggg accaccagct gcgttgcggg cgtgtctttg ccggcaaggt
    53581 acagggcgaa ggcgacaccc agcgcaatga ccgacatcca gaaatacttc caacgagcga
    53641 tcaacccttt ggtgtcttta cgcaccactt tgcgataatc atcaacaaag ccaataacac
    53701 cgtaacctac cagcaccacc aacacgcacc agacgtacgg attggacggg taagcccaca
    53761 gcagtacgga gatcacaatc gccgtcagga tcataatccc gcccatggtc ggcgtaccgc
    53821 gcttgctgaa gtgtgattca ggaccgtcgt tacgcaccac ctgaccaaag gaaagttttt
    53881 gcaaatgagc tatcatacgc gggcccatcc acaatgagat gaacagcgcg gtcagcaggc
    53941 tgacgatggc gcgaaacgtc agataggaaa agacgttaaa gccggaataa tatttgacca
    54001 aatgttcggc cagccaaact aacatgtccc attctcctgt aaagcgcgta ctacctcttc
    54061 catggcggca ctacgtgaac ccttaactaa aatcgtaatt acctgttgct cagcaatcag
    54121 tgatttaaga cgcgtaatta acgcagtttt atcagcaaaa tgttcgccaa cgccgctggc
    54181 ggtgctgata gcatggcttt gtttacccac gcttaacacg cggtcaatac cagcagcttt
    54241 tgccgcctcg cccacctgta catggcaggc ttcgctttca gcgcccagtt ccgccatatc
    54301 gcccaccacc agcacgcggt agcccggcat ttcagccagt acctgaacag cggcggtcat
    54361 tgaaccgaca ttggcgttgt aggagtcgtc gagcagcaac tggttttctg ccagttggat
    54421 ggggaacaga cggcctggaa cagctttcag atttgccagc cccgctttga tagcatcaag
    54481 cgttgcgccc acggacatgg agagcgcagc ggctgccagc gcattcgcaa tattgtgacg
    54541 ccccggcaac ggcagcagaa catcgacgct accggttggg gtttgtaggg taaattccgt
    54601 accgtgcgag gtcacatgga tattggtggc ggtgaaatcg ctgttggcgg cattgggtga
    54661 gaaacgccac actttgcgtg agccaattac gctctgccag ttcagccagt cgttgttgtc
    54721 ggcgttcata atggcgatac cgttttccgg caggccgcta aagatttcac ctttcgcttt
    54781 cgcgacaccc gcaagcgagc caaaaccttc cagatgcgcc gctgccaggt tgttgaccag
    54841 cgcagcttcc gggcgagtca gactcacagt ccaggctatt tcgccctgat ggttcgcgcc
    54901 aagttcaata actgcgtaat cgtattccgg cgtcaagcgc aacaacgtca tcggtacacc
    54961 gatgtcgttg ttgagattgc ctgccgtata aagcgtgttg ccgcactggc ttaaaatcgc
    55021 cgccgtcatc tctttaacgg aggttttgcc ggaggaacct gtcagagcaa ccacgcgcgc
    55081 cggaacttgc tggcgaaccc atgcagccag ttcaccaaac gccagacgcg tatccttgac
    55141 gattaactgc ggcaggtcga tatccagcgg acggctgacc agtagtgcgc ccgcgccgcc
    55201 agctttcgcc tggtcggcaa aatcatgagc atcgaaacgt tcgcctttca gggcaacaaa
    55261 caggcagccc ggcgtcagtt ttcgggtatc agtggttaca gcatcaaggg tgatatctgc
    55321 accttgcagt tcaccgttga gaatgtcggt aagttggcta agggttacgc taatcatgca
    55381 atcaccccca gcagacgcgc caccgtgacg cgatcggagt agtccagacg ctgattgcca
    55441 acaatctggt aatcttcatg gcctttgccc gcgaccagta ccacatcatt ctctttagcc
    55501 tgcataacgg cgcaagtcac cgcttcagca cggccttcca tcactttggc atgtccggca
    55561 tctaacattc ccgccagaat atcgttgatg atggcacgcg gttcttcggt acgcgggtta
    55621 tcgtccgtca ccaccgccac gtcagcaaac tcttcggcaa ttgcgcccat cagtggacgc
    55681 ttacctttat cgcgatcgcc accacagcca aagacgcacc acagcttgcc cgcacagtgc
    55741 aggcgcgccg cctgtaaggc tttttccagt gcatccggcg tatgcgcgta atccaccacc
    55801 accgtcggtt tgcctggcgc agtgaacact tccatacgtc cgcaaaccgg ttgcagacgc
    55861 gcggcggttt tcagcagatc agccagtgga tagccgagtg ccagcagtgt cgccagcgcg
    55921 agcagcaggt tgctgacgtt aaaagcgccc atcagatggc tttcaatttc gccatcgccc
    55981 caacttgagc taaagcgaat cgtcgcaccg ctgtcgtgat agttcacgtc gatcgctttt
    56041 aaccagcgtc cgtgacagtt cggattaata tgatcttcca ttgataccgc aaccgcgtcc
    56101 ggcagttttg ccagccagcg gcggcccact tcatcgtcgg cgttaataat cgcctgaccg
    56161 caatgatgtt cagaataaag cagccatttc gcagcttcgt agtgttccat atcaccatga
    56221 taatcaaggt gatcgcggct taagttggta aagaccgacg ccgcaaattt caatgccgcc
    56281 acacggtgct gtaccagccc gtgggaggaa acttccattg cgcaaaacgt cgcgccctgc
    56341 tccaccagcc ccgccagctc atgctgaaca tcgactgcgg aaccggtagt attttctgtc
    56401 gggatcactt tcccaagcag accgttacca acggtgccca ttaccgcgct ggtttcgcca
    56461 agcagttggc tccactgcgc cagcagctgg gtagtcgtgg ttttgccgtt ggtgcccgtt
    56521 acgcctacga gacgtaaatt gtcagagggt tcatggtaaa agcggcccgc cagtgcagat
    56581 aaacgctcat tgagctggct gagatagatg accggtacgc cgtgcatttc acggatttca
    56641 ccgtcggtcg cctcatcttt cgctttcgcc tctgcaataa tggcagccac accttgcgct
    56701 atcgcctgcg ggatatatcg acgcccgtcc gcctgatgac ctactacagc tacaaagaga
    56761 tcgcccgccg cagccacacg gctgtcgagt gtcatctctc gcagtgctcg cgaaggtgcg
    56821 tctggcaccc acggagcaag aaggtcgcgc aaattacgat ctgccacctg tcccctcgcc
    56881 ttgattaatc acaaattcat ttttatcgcc cgttgtcagc gcatccggct cgatgttcat
    56941 ggtacgcaat acgccgccca tgatggcacc aaagaccggc gcggaaacgg cgccgccgta
    57001 gtatttaccc gcctgcggat cgttgataac aacaaccagc gcgaagcgcg gctgactcgc
    57061 aggcgcaacg cctgcggtat aagcaatata tttattgatg tagcgaccgt ccggcccgac
    57121 ctttttcgcg gtaccggttt taatggcgat acgatagcct ttaatcgccg ccttcacgcc
    57181 gccgccgcct ggtagcgcca cgctttccat catatgcacc acagtgcgga caatggatcc
    57241 cgggaagaca cgttcaccgg gaaccggggg gtcaactttg gtaatcgaca gtgggcgata
    57301 aatgccgtag ctgccgatag ttgcgtagac tcgcgctaac tgtaacggtg ttaccattag
    57361 cccgtagccg aaagagaagg tggccctctc tatgtcagac caccgttgtt tttgaggata
    57421 taagccactg cgttctccga ccaaccccaa attggtcgct tttcccagtc caaaacgtga
    57481 gtaagtatct actaacgctg aggacggcat cgctaacgcc agcttggaaa caccgacgtt
    57541 actcgacttc tgtaataccc cggtcagggt taattcgctg tagcgtgcca cgtctttgat
    57601 ttcgtggccg ttaattcgat aaggaatggt attgagtacc gagttttccc gcaccacgcc
    57661 acgttgcaac gcggtcatta ccaccatcgg tttaaccgtt gagcccggtt caaacacgtc
    57721 ggtgatggta cggttacgca tcgcctcttt cggcgtgccg ctcagattgt tagggttgta
    57781 tgacgggctg ttagccatcg ccagcacttc accggtgttg acatccacca gcacggcgct
    57841 accagattca gccttgttaa aggccaccgc gttgttcagt tcgcgataaa ccagcgcctg
    57901 caggcgttca tcaatactca gcgccaggtt gtgcgctgcc tggctgtcag tagaagaaat
    57961 atcttcaatt acgcgaccat agcggtcttt acgcacaatg cgctcacccg gctgcccggt
    58021 aagccattta tcgaaactct tctcaacgcc ctcaatccct tgactatcga cgttagtaaa
    58081 gccgatgagg tgagcagtca cttcgccgga cggatagtaa cggcgagact cttcacgcag
    58141 atgaatcccc ggcagtttca gttttttgat gtagtccgcc atgtcagggt tcacctgacg
    58201 cgccagataa ataaaacgcc ctttcgggtt ggcgttaatg cgggctgaaa gctgatccag
    58261 cggaatattg agcgcgttag ccagcgcctt ccagcggtca ccgacgctga taccgccagc
    58321 gtcatgcact tctttcgggt cagcccaaat cgcttttacc ggcacgctca ccgctaacgg
    58381 gcgaccagaa cggtcagtaa tcatgccgcg ggaggtggaa acttgctgaa cgcgaagaga
    58441 acgcatgtcg ccctctttca ccagcatatc cggggagata acttgtaacc acgctacgcg
    58501 tccgagcaga aaagccagcg ccaggagaat acagccgcat aacaacgcaa aacgccaact
    58561 gataaagttg gcatgttctt cctgacgttt tggtttctgc gttttcgccg ctgctttcat
    58621 gcgtcgcgtt tatccttatt tttgcactac gatattttct tgtgacggat caacatgctg
    58681 catttgcagc ttttccgtgg cgatcctttc cacccggcta tggtcgccga gcgcattctc
    58741 ttcaaggatc aggttgcgcc attcaatgtc taaagcatct cgctccagca ccagttgttc
    58801 gcgctgagcg gtcagtaaac gggtatggtg cgccgtggtt accacagtca ccgccgtcaa
    58861 aataatgcaa atgaacaggc agagtggcag cttcccaaat cgcaaaagat cgtcaccgat
    58921 aacaccaggc aatgcatggc gctcgtggct tcccatcgat cctttaactt tgcttagagc
    58981 ttctgtcact ctgctgatca tgcattcgtc ctctctgcaa tacgcagaac tgaactacgg
    59041 gcacgagggt tctcagccac ctcttcttcg cccggcatta acttgcctag tgctcgcagc
    59101 tgacggccac ccagtttttt gagctgctct tcagtcatcg gtaaccctgc cggaacttgc
    59161 ggaccgcggc tgttttcacg cataaaacgt ttcacaatac ggtcttccag cgagtggaag
    59221 ctgatgatcg aaagccgccc acccggggcc agcacgttga gcgagctttt tagcgcctgc
    59281 tctatctcct ccagttcact gtttacccaa atgcgcaccg cctggaaggt acgggtcgcg
    59341 ggatgtttaa acttatcttt caccggcgtt gcaacagcca cgacttccgc cagttctttg
    59401 gtgcgggtca tcggctgttc gcggttacgc tcgacaatgg cgcgggcaat gcgtttggca
    59461 aaacgctctt caccataggt tttcaatacc caagcgatat cggcttcttc tgcggtttgt
    59521 agccattcag cggctgactg cccacgggtt gggtccatac gcatgtccag cggaccatcg
    59581 cgcataaagg aaaagccgcg ctcagcatca tcaagttgcg gtgaagagac gccaagatcg
    59641 aggagaatgc cgtcgatctt gccgataaga tcgcgctcgg caacgtattc gcccagcgcg
    59701 gagaaaggtc cgtggatgat ggagaagcgc ggatcatcaa tagtcttcgc aacggcgata
    59761 gcctgcgggt cgcgatcgat cgccagcaaa cgcccctctt cgccaagctg cgagaggatc
    59821 agacgtgagt gaccaccgcg accaaaagtc ccatcaatgt agatgccatc aggacggata
    59881 ttgaggccat taacggcttc atccagcagc accgtagtat gtttatagtt ttccatcatt
    59941 ttatagagac aagtcctgca gtcgctccga taagtctccg gtagccaact gctctgcgtc
    60001 gatatcttcc ttgacctgtt gatgccaggt tgtttcatcc cacagctcaa acttgttgaa
    60061 ctgtccaacc agcatcactt cttttgtcag cccggcatgt tgccgcagta ctggcgcgat
    60121 taacaatcga cctgcgccat ccatctgaca ttcgctggca tgacctaaca gtaggcgctg
    60181 cacacggcgc tcaaccgggt tcatgctcga cagacgcgat aatttttgct cgataatttc
    60241 ccattcaggc agggggtaaa gcagcaggca cgggtgatga atgtcaatgg tgcaaaccat
    60301 ttgaccgaca gcgttctcaa gcagctgttc ccggtaacgg gtaggcactg ataagcgccc
    60361 tttgctgtcg agattgacta acgttgctcc ccggaacatg ccagcctcac cccttattcc
    60421 cactttgccc cacaatttcc cactgaaagg agtttacgga gcggaggaaa agcttgtcaa
    60481 gccagtcaca aggcataccg aggtaaaaaa cccgatgttt acggctaata tcagcgttgt
    60541 ttattttacg tccaacgtgc gcagcaaatt aacgttatga atagctgtaa gaaaaaatcc
    60601 ctcatcgtca tcaccgtttt aaaatgactc actattttcc acagcaaata taaagtgtca
    60661 gtttgcgacg cgagcggcat tttaggacat atcttccccg gttaacagtc cctgttgcgt
    60721 ctgtgtggcg cttgatgcca gcgggtttgc gcaaaagcaa agaaaagtgc ttgttaaatc
    60781 ggcaattcat ctctgcatgt aacaaaataa tgcaaaaacg cgctgtcgat atcacttaat
    60841 tattgtgaat ttcagctggt gagttaatta acgggcgggt ttaaaagaac ttatcaataa
    60901 tagtcaggaa tagtcttatt tactttaagg atattgatgt ccattcccgt actctacg
//
LOCUS       DAJVQG010000006        59825 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJVQG010000006 DAJVQG010000000
VERSION     DAJVQG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59825
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.006"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..262)
                     /locus_tag="OQM83_000343"
     CDS             complement(<1..262)
                     /locus_tag="OQM83_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS1518700.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
     gene            369..665
                     /locus_tag="OQM83_000344"
     CDS             369..665
                     /locus_tag="OQM83_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1518701.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(799..1224)
                     /gene="arsC"
                     /locus_tag="OQM83_000345"
     CDS             complement(799..1224)
                     /gene="arsC"
                     /locus_tag="OQM83_000345"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCS1518702.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1237..2526)
                     /gene="arsB"
                     /locus_tag="OQM83_000346"
     CDS             complement(1237..2526)
                     /gene="arsB"
                     /locus_tag="OQM83_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCS1518703.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2580..2933)
                     /gene="arsR"
                     /locus_tag="OQM83_000347"
     CDS             complement(2580..2933)
                     /gene="arsR"
                     /locus_tag="OQM83_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCS1518704.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            complement(3472..3645)
                     /locus_tag="OQM83_000348"
     CDS             complement(3472..3645)
                     /locus_tag="OQM83_000348"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1518705.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            3673..3756
                     /locus_tag="OQM83_000349"
     CDS             3673..3756
                     /locus_tag="OQM83_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCS1518706.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3810..5162)
                     /gene="gorA"
                     /locus_tag="OQM83_000350"
     CDS             complement(3810..5162)
                     /gene="gorA"
                     /locus_tag="OQM83_000350"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCS1518707.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5234..6076)
                     /gene="rlmJ"
                     /locus_tag="OQM83_000351"
     CDS             complement(5234..6076)
                     /gene="rlmJ"
                     /locus_tag="OQM83_000351"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCS1518708.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6279..8321
                     /gene="prlC"
                     /locus_tag="OQM83_000352"
     CDS             6279..8321
                     /gene="prlC"
                     /locus_tag="OQM83_000352"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCS1518709.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKSVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8329..9081
                     /gene="rsmJ"
                     /locus_tag="OQM83_000353"
     CDS             8329..9081
                     /gene="rsmJ"
                     /locus_tag="OQM83_000353"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCS1518710.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9130..10599)
                     /gene="dtpB"
                     /locus_tag="OQM83_000354"
     CDS             complement(9130..10599)
                     /gene="dtpB"
                     /locus_tag="OQM83_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HCS1518711.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(10917..11351)
                     /gene="uspA"
                     /locus_tag="OQM83_000355"
     CDS             complement(10917..11351)
                     /gene="uspA"
                     /locus_tag="OQM83_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCS1518712.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11742..12077
                     /gene="uspB"
                     /locus_tag="OQM83_000356"
     CDS             11742..12077
                     /gene="uspB"
                     /locus_tag="OQM83_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCS1518713.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12321..13820)
                     /gene="pitA"
                     /locus_tag="OQM83_000357"
     CDS             complement(12321..13820)
                     /gene="pitA"
                     /locus_tag="OQM83_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCS1518714.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14052..15254
                     /locus_tag="OQM83_000358"
     CDS             14052..15254
                     /locus_tag="OQM83_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCS1518715.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(15570..16553)
                     /locus_tag="OQM83_000359"
                     /pseudo
     CDS             complement(15570..16553)
                     /locus_tag="OQM83_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            16953..18560
                     /locus_tag="OQM83_000360"
     CDS             16953..18560
                     /locus_tag="OQM83_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCS1518716.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKKYRSNTVEVN
                     MLNDFSQIDNDLGLNIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKSDITYKVNGLS
                     IIPENFFTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNAN
                     TAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHENMASSAYIHKNNKLLYN
                     LLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMDG
                     NKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCHYDSETKVL
                     TSHHGIIRNEENECYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRS
                     NAVASTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTM
                     GLNSWKIAPNERGDKKGVPGLSCFQPQ"
     gene            18822..20444
                     /locus_tag="OQM83_000361"
     CDS             18822..20444
                     /locus_tag="OQM83_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCS1518717.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            20810..21877
                     /locus_tag="OQM83_000362"
     CDS             20810..21877
                     /locus_tag="OQM83_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1518718.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELSWPDDLVVRLPQ"
     gene            21874..24609
                     /gene="rbbA"
                     /locus_tag="OQM83_000363"
     CDS             21874..24609
                     /gene="rbbA"
                     /locus_tag="OQM83_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCS1518719.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            24609..25733
                     /locus_tag="OQM83_000364"
     CDS             24609..25733
                     /locus_tag="OQM83_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1518720.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            25806..26079
                     /locus_tag="OQM83_000365"
                     /pseudo
     CDS             25806..26079
                     /locus_tag="OQM83_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            26076..26435
                     /locus_tag="OQM83_000366"
     CDS             26076..26435
                     /locus_tag="OQM83_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCS1518721.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(26555..26956)
                     /gene="nikR"
                     /locus_tag="OQM83_000367"
     CDS             complement(26555..26956)
                     /gene="nikR"
                     /locus_tag="OQM83_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCS1518722.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(26962..27768)
                     /gene="nikE"
                     /locus_tag="OQM83_000368"
     CDS             complement(26962..27768)
                     /gene="nikE"
                     /locus_tag="OQM83_000368"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCS1518723.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(27765..28529)
                     /gene="nikD"
                     /locus_tag="OQM83_000369"
     CDS             complement(27765..28529)
                     /gene="nikD"
                     /locus_tag="OQM83_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCS1518724.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(28529..29362)
                     /gene="nikC"
                     /locus_tag="OQM83_000370"
     CDS             complement(28529..29362)
                     /gene="nikC"
                     /locus_tag="OQM83_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCS1518725.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(29359..30303)
                     /gene="nikB"
                     /locus_tag="OQM83_000371"
     CDS             complement(29359..30303)
                     /gene="nikB"
                     /locus_tag="OQM83_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCS1518726.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(30303..31877)
                     /gene="nikA"
                     /locus_tag="OQM83_000372"
     CDS             complement(30303..31877)
                     /gene="nikA"
                     /locus_tag="OQM83_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCS1518727.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTPQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(31988..32575)
                     /gene="acpT"
                     /locus_tag="OQM83_000373"
     CDS             complement(31988..32575)
                     /gene="acpT"
                     /locus_tag="OQM83_000373"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCS1518728.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(32630..33679)
                     /locus_tag="OQM83_000374"
     CDS             complement(32630..33679)
                     /locus_tag="OQM83_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCS1518729.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            33811..35028
                     /locus_tag="OQM83_000375"
     CDS             33811..35028
                     /locus_tag="OQM83_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1518730.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(35032..35589)
                     /gene="dcrB"
                     /locus_tag="OQM83_000376"
     CDS             complement(35032..35589)
                     /gene="dcrB"
                     /locus_tag="OQM83_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCS1518731.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(35662..36327)
                     /locus_tag="OQM83_000377"
     CDS             complement(35662..36327)
                     /locus_tag="OQM83_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCS1518732.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            36548..36793
                     /gene="tusA"
                     /locus_tag="OQM83_000378"
     CDS             36548..36793
                     /gene="tusA"
                     /locus_tag="OQM83_000378"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCS1518733.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(36895..39093)
                     /gene="zntA"
                     /locus_tag="OQM83_000379"
     CDS             complement(36895..39093)
                     /gene="zntA"
                     /locus_tag="OQM83_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCS1518734.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(39167..39793)
                     /locus_tag="OQM83_000380"
     CDS             complement(39167..39793)
                     /locus_tag="OQM83_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCS1518735.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            39934..40293
                     /locus_tag="OQM83_000381"
     CDS             39934..40293
                     /locus_tag="OQM83_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCS1518736.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(40296..40565)
                     /locus_tag="OQM83_000382"
     CDS             complement(40296..40565)
                     /locus_tag="OQM83_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCS1518737.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(40555..41151)
                     /gene="rsmD"
                     /locus_tag="OQM83_000383"
     CDS             complement(40555..41151)
                     /gene="rsmD"
                     /locus_tag="OQM83_000383"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCS1518738.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            41301..42797
                     /gene="ftsY"
                     /locus_tag="OQM83_000384"
     CDS             41301..42797
                     /gene="ftsY"
                     /locus_tag="OQM83_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCS1518739.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            42800..43468
                     /gene="ftsE"
                     /locus_tag="OQM83_000385"
     CDS             42800..43468
                     /gene="ftsE"
                     /locus_tag="OQM83_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCS1518740.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            43461..44519
                     /gene="ftsX"
                     /locus_tag="OQM83_000386"
     CDS             43461..44519
                     /gene="ftsX"
                     /locus_tag="OQM83_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCS1518741.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINDLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            44764..45618
                     /gene="rpoH"
                     /locus_tag="OQM83_000387"
     CDS             44764..45618
                     /gene="rpoH"
                     /locus_tag="OQM83_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCS1518742.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            45889..46992
                     /gene="livJ"
                     /locus_tag="OQM83_000388"
     CDS             45889..46992
                     /gene="livJ"
                     /locus_tag="OQM83_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCS1518743.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(47191..47574)
                     /gene="panM"
                     /locus_tag="OQM83_000389"
     CDS             complement(47191..47574)
                     /gene="panM"
                     /locus_tag="OQM83_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCS1518744.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            47998..49107
                     /gene="livK"
                     /locus_tag="OQM83_000390"
     CDS             47998..49107
                     /gene="livK"
                     /locus_tag="OQM83_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCS1518745.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            49155..50081
                     /gene="livH"
                     /locus_tag="OQM83_000391"
     CDS             49155..50081
                     /gene="livH"
                     /locus_tag="OQM83_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCS1518746.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            50078..51355
                     /gene="livM"
                     /locus_tag="OQM83_000392"
     CDS             50078..51355
                     /gene="livM"
                     /locus_tag="OQM83_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCS1518747.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            51352..52119
                     /gene="livG"
                     /locus_tag="OQM83_000393"
     CDS             51352..52119
                     /gene="livG"
                     /locus_tag="OQM83_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCS1518748.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            52121..52834
                     /gene="livF"
                     /locus_tag="OQM83_000394"
     CDS             52121..52834
                     /gene="livF"
                     /locus_tag="OQM83_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCS1518749.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            53118..54182
                     /gene="ugpB"
                     /locus_tag="OQM83_000395"
                     /pseudo
     CDS             53118..54182
                     /gene="ugpB"
                     /locus_tag="OQM83_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            54280..55167
                     /gene="ugpA"
                     /locus_tag="OQM83_000396"
     CDS             54280..55167
                     /gene="ugpA"
                     /locus_tag="OQM83_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCS1518750.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            55164..56009
                     /gene="ugpE"
                     /locus_tag="OQM83_000397"
     CDS             55164..56009
                     /gene="ugpE"
                     /locus_tag="OQM83_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCS1518751.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            56011..57064
                     /gene="ugpC"
                     /locus_tag="OQM83_000398"
                     /pseudo
     CDS             56011..57064
                     /gene="ugpC"
                     /locus_tag="OQM83_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
     gene            57061..57804
                     /gene="ugpQ"
                     /locus_tag="OQM83_000399"
     CDS             57061..57804
                     /gene="ugpQ"
                     /locus_tag="OQM83_000399"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCS1518752.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(57791..58231)
                     /locus_tag="OQM83_000400"
     CDS             complement(57791..58231)
                     /locus_tag="OQM83_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCS1518753.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            58351..>59825
                     /gene="ggt"
                     /locus_tag="OQM83_000401"
     CDS             58351..>59825
                     /gene="ggt"
                     /locus_tag="OQM83_000401"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCS1518754.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVL"
BASE COUNT        14591 a        15106 c        16134 g        13994 t
ORIGIN      
        1 acccggcttt cgctgacaac aatccacgta acatgggtat tgtcgagcaa ctacagaacg
       61 gggtaacggc tcccaacaga gcagccagga gatagcctct cccttttctg gcccccatca
      121 tctgctggat tttatggtct ggcacctttt gtcttatcag gctgacaccg gcactaatca
      181 ctataaacag caacgaaagc tcgacggcga gaaacacaaa catctctgcg gcatcttgca
      241 gcatcgcaag ccaactactc atacgtaacc tcattggata aatctatatt tccagaataa
      301 tcgaaatgaa attcagatca agatatttct ggtattatcg aaatatcaac cagacagagg
      361 ggtgtcatat gcagttggaa gaagtcgcta aagcgctgaa agagctaggt cacccgaccc
      421 gtttgttcat atttaaacat cttgtcaaag ccggggaaca agggttgccg gtcggtgagt
      481 tgcaaaagca gcttgggata cctggctcca cactcagcca ccatatatca gcactggtat
      541 cggtaggact ggtcaaacaa aaccgtgaga gtcggacgct gatgtgtgtt tcgcaatacg
      601 caatgctgga agcgataatt gaattcttac gcgaggagtg ctgcgtaaat agcaaaattg
      661 cctgatgcgc tacgctctac atatttcatg ccatatattg aatttgcacg gttttgtagg
      721 tcggataaga cgcgttagcg tcgcatccga cataaacaaa gcgcacttta ccaactatcg
      781 gcagtggata tcgccgcttt atttcaggcg cttacccgct tcatcaacca ctttctcgcc
      841 atcttccttg gagaatgcgc ctttttgcgc atctggcaga atttccagca ccacttctga
      901 agggcggcac aggcgagttc ccagcggcgt caccacaatc gggcgattaa tcagaatcgg
      961 gtgctgaagc ataaagtcga ttaaccgatc gtcagtaaat ttatcttccg caaggcccag
     1021 ctcctcatac ggttcgacgt ttttacgcag cagcgcgcgt acggaaatcc ccatatcggc
     1081 aatgagtttg accagttcat cgcgcgttgg cggagtttcc agataatgga taatggtcgg
     1141 ttctgtgccg ctgttgcgga tcatctccag cgtattacgc gacgtgccgc aggccgggtt
     1201 gtgataaatg gtaatgttgc tcatatcagt atctcattac aaagtgaaag agagacgtag
     1261 cgccagcgca gccagcgtca caaacagcac aggcagggtc atgataatcc ctgtacggaa
     1321 gtaatatccc cagctgatag tcatattctt ctgcgaaagt acgtgcagcc agagtagcgt
     1381 agccaggcta ccaattgggg taattttcgg tcccaaatcg cagccaatca cattggcata
     1441 aaccatcgct tctttgataa cgccagatgc cgtgctgcca tcaatggaca acgcgccaac
     1501 cagtaccgtc ggcatattgt tcataataga agagaggaag gcggtgagga atccggtgcc
     1561 gagcgtcgcg gcccacaggc cgttatccgc cagcacgttg agtacgccag aaagatattc
     1621 cgttaatccg gcattgcgca ggccataaac caccagatac atgccgagcg agaagatgac
     1681 aatctgccag ggggcaccgc gcaggacttt acccgtatta atcgcatgac cgcgtttagc
     1741 gacgacaaat aatatcagcg cgcccacagc tgcaatggcg ctcaccggaa tgccgagcgg
     1801 ttccaggacg aaaaatccca ccagcagaag cagtaaaaca acccagccag ttttgaacgt
     1861 agcaggatct ttgatcgctt ctgcgggaga tttcagcagc gccatatcgt agttctgcgg
     1921 aatatctttg cgaaaataga gatgtagcat caccagcgtg gcaacaatcg cggcgatatc
     1981 caccggcacc atcaccgagg cgtattcgcg aaagccgagg ccaaagaaat cagcggaaac
     2041 gatattcacc aggttggaga caataagcgg caggctggcg gtatcggcaa tgaatccggc
     2101 cgccatcacg aacgccagcg tcgtgccttt actgaatcct aaagccagca gcatggcgat
     2161 gacaatcggc gtcaaaataa gcgccgcgcc atcattggca aacagggcgg caacggcagc
     2221 accgagcagg acaatccagg taaacagcaa gcgaccacga ccattacccc agcgtgagac
     2281 gtgcagcgcc gcccattcaa aaaagccgga ctcatccagc agcaggctga tgataatgac
     2341 ggcgataaac gcagccgtcg cgttccagac gatattccac accaccggaa tatcacccgg
     2401 atgaaccacg cccgtaacta acgccagtac tgcgccgagc gttgcactcc agccgatgcc
     2461 taaacctttc ggctgccaga taaccaatac gatggtcagg acaaagatag cgcctgccag
     2521 taacataatg cctcccggat aaaacacatc tgaaaattca tatgtgttta gctaaatttt
     2581 taactgcaaa tgttcttact gtccccggaa cagttttgtc gagccaggtt gcggacaatc
     2641 gcctgaacct tttcctgttc acatcgccag gcctcatcaa taattttcgc cgcccatgct
     2701 ggaatatgcg gtgataagcg gtaatgaacc cacttacctt gcttgcggtc cagcaatagc
     2761 ccgctttcac gaagcaatgc caggtggcgg gagatcttgg gctgcgactg atcgagggca
     2821 gtacaaagat cgcagacgca taactctccc agctcgctga gcagtaaaac gatgcccaga
     2881 cgggtttcat cagcaagaat tttgaacaat tggatgggta acagaaatga catattgcgc
     2941 tcctgattgt ggcaggtagt gtctctcttc gaagcggata agtcaaaaac atatatgact
     3001 taacgaatgt gtaagtgcag aggaaggtaa taggtgtgaa ttttgagttg gctattcatt
     3061 tgaaaggcgg tttgaatcag gacgtaaaaa aaccgacttt gcgtcggttt tttactcttc
     3121 agcctgagtt ggtggctctg gctggagtga gaatgcccac atctaacggt tcaatactat
     3181 tagccagtgg ctaacattgc aagaattagc gatttcttca gctggcggag ggggcacttc
     3241 ggtatatatc gcaatgacga tttatatact ttcactgggt catcgtcata ttaagccttg
     3301 tccgaatccg gatgtaaaaa aaccgacttt gcgtcggttt ttttactctt cagccctgag
     3361 ttggtggctc tggctggagt gagatagtca tcatctaacg catcaatgct aataccatac
     3421 tgaaactatt gcaaggacat gctggtttta taacctgaat gtactgtatg attatccagt
     3481 tagctctgag gcattttcac tctggtaatg cgcataaacg ctttcaaagt cctggtcaga
     3541 agtacgggtg gtgccgttaa ctgatgctct ggccggagtg agatagtcgt catctaacaa
     3601 tgagacatgc gccgtgacag gcagtggatg agtaagcgga tgcattctca ctccatcgca
     3661 tggagaaaac gggtgattga taaagcaatc atcgttcttg gagcgttaat tgcgctgctg
     3721 gaactgatcc gctttctgct tcagcttctg aactgatagc ggaaacgtaa ttaagggcta
     3781 agagcacact actcttagcc ctttaacatt tagcgcatgg tcacgaactc ttctgccgcc
     3841 gttgggtgaa tggcgacggt attgtcgaag tcttttttgg ttgcgcccat cttcagcgcc
     3901 accgcgaagc cctgcaacat ttcgtccata ccaaagccaa tgccgtgaat accgacaatc
     3961 ttctcttctg gcccaacgca caccagcttc atgcggcacg gctggcggtg agtggtgacg
     4021 gcggtataca tcgcggtgaa agaggattta tacactttca cctgatcgtc gccgtactgc
     4081 tcgcgcgcct gcggttccgt taaaccaaca gtaccaatcg gcggatggct gaagaccacg
     4141 gtcggaatgt tgctgtaatc cagatgctca tccggcttgt tattaaacag gcgttcagag
     4201 agacggcgac ccgctgcaac tgctaccggt gtcagttcca ctgcacccgt gttatcgccc
     4261 accgcgtaaa taccttcaat attggtgttt tgatatttat cgacgacgat atagcctttt
     4321 tcgttagttt taacgccagc ggcttctagg ttgatgttgt cattggcagg ctcgcgacca
     4381 atcgcccaaa tcaggcaatc caccgtttca ctgcgaccat cttccagctc cagcgtcagg
     4441 ctaccatcgg catttttcac taccgctttc gggatggcgt tggtgtgcag ctgcgggcct
     4501 tcggcgttca tcacttcgac cagcgtttcg gaaatcatcg ggtcgaagct gcgcagcggc
     4561 gcatgtttac gcacaaacag atgcgttttc gcgccgaggc cgttaatcac gcccgccagc
     4621 tcaacggcga tgtaacccgc gccaacaacc gccacgcgct ctggcaaagc aggaagggcg
     4681 aagaagccat cagaatcaat accgtattcc acgcccggaa tatccgggtg gctcggacga
     4741 ccgcctgtgg cgatcagaat atgatcggcc gtgatggttt cgccgtttac ctccagcgtt
     4801 ttggcatcaa cgaagcgggc aaagcctttg attacatcaa cgttattttt accgagcacg
     4861 ttttcatagg aagtatgaat acggtcgata taggcggtac ggctggcgat caacgtttcc
     4921 cagttgaact tgttgatggt ggtatcgaaa ccataatcag ggccgtacat atggatcgct
     4981 tcacggattt gcgccgcgtg ccacatcact tttttcggca cgcagccgac gttcacgcag
     5041 gtgccgccca gctctttggc ttcaatcagt gcgcatttct ggccgtacat agccgcgcgg
     5101 ttgatggagg cgataccgcc gctgccgccg ccgatggcga tgtaatcata gtgtttagtc
     5161 atgacagagt gtccttatcg ttgattaccg cgattgtagc gcgagacgta ataggtgcca
     5221 gcaatggctg caattactcc ggcacgatcc agcttacggt ggcgtgcccg gtgcctgccg
     5281 gaaccagttt gctgtgcagc cacggcagca cgttattcat ctgctgttcc agtttccacg
     5341 gcgggttaat cacaatcatg ccggaagcgg tcatgccacg gcgatcgctg tctggcagta
     5401 ccgccagttc aatttgcaga attttgcgaa taccggtcgc ttccagatcg tggatcatgc
     5461 gcttaatttg ctgacgcagc accaccggat accacagtgc gtaagtacca gtggcgaaac
     5521 gtttgtaacc ttctgctatc ccgctgacca ccgcctgata gtcggttttc atttcatacg
     5581 gcgggtcgat aaggattaaa ccacggcggg aaaccggcgg aagtttggcc ttaagctgct
     5641 ggaaaccgtc ggctttttcg acgcgcgcac ggctgtcttt ctgaaattca gaacgcagca
     5701 acgggtaatc gctcgggtgc agttcggtca gttgcaggct gtcttgttcg cgcagtagct
     5761 ggcgagcaat caacggcgac ccggggtagt aacgcaactg accgctacgg ttgaagtgtt
     5821 ttaccacatt gatgtacgcc tccagttctg cgggcaaatc gtcctgctgc cagatacggg
     5881 cgataccttc gagatattcg ccggtacgct cggcatgttc gctgcctaac tgataacgcc
     5941 cggcccctgc gtgggtatcg agatagagaa acggtttatc tttctctttc agcgactcga
     6001 tgatcaggct ctgaacggta tgtttaagga cgtcggcgtg gttgccagcg tgaaagctgt
     6061 ggcgataact gagcatgggt aaaggtgttc cggtaagtaa aaatcggccc gtattcgggg
     6121 cgcacaaaag cgtatcagga cagtataccg aaaagaggcc gccgccgcga aagcgtaacg
     6181 tttctcattg aaattcacta cacttaaccc catgctacac acattatgta aagcgcctgt
     6241 tgagcgcttc cttaacctct ttaaccagga ctgcgcgaat gacgaatccg ttactgactc
     6301 cctttgaatt gcctccgttt tctaaaattc tcccggaaca tgtcgttcca gccgtgactc
     6361 aggcgctgaa cgattgccgc gaaaatgtgg cgcgcgtagt agcgcaaggg gcaccgtaca
     6421 cctgggaaaa tctctgccag ccgttggcgg aagtggacga tgtgttgggg cgtatcttct
     6481 ccccggtcag ccacctgaac tcggtgaaaa atagcccgga actgcgtgaa gcctacgagc
     6541 aaaccctgcc gctgctgtcg gaatacagca cctgggtagg gcaacatgaa gggctgtata
     6601 aagcgtaccg cgacctgcgc gatggcgatc attacgccac gctgaacacg gcgcagaaga
     6661 aagcggttga taacgccctg cgtgattttg aactctctgg cataggtctg ccgaaagaga
     6721 aacagcagcg ttacggcgaa attgctaccc gtctttctga actgggcaac cagtacagca
     6781 acaacgtcct cgatgcgacg atgggctgga ccaaactcgt taccgacgaa gcggggctgg
     6841 cggggatgcc agaaagcgcg ctggctgcgg caaaagccca ggccgaagcg aaagagctgg
     6901 aaggctacct gctgacgctg gatatcccaa gctacctgcc ggtaatgacc tactgcgaca
     6961 accaggctct gcgcgaagag atgtatcgcg cttacagcac ccgcgcctcc gatcaaggcc
     7021 cgaacgccgg taagtgggat aacagcaagg tgatggaaga gatcctcgcg ctgcgtcacg
     7081 aactggcgca actgctgggc tttgaaaact acgcctttaa atcccttgcc actaaaatgg
     7141 cagaaaaccc gcagcaggtg ctggatttct taaccgatct ggcaaaacgc gcgcgtccgc
     7201 aaggcgaaaa agagctggag caactgcgtg ccttcgctaa agccgaattt ggtgtcgatg
     7261 agttgcagcc gtgggatatc gcttactaca gcgaaaaaca gaaacagcac ctctacagca
     7321 tcagcgacga gcagctgcgt ccgtacttcc cggaaaacaa atcggttaac ggcctgtttg
     7381 aagtggtgaa acgtatttac ggcatcaccg ctaaagagcg taaagaggtt gatgtctggc
     7441 atccggatgt acgtttcttc gaactgtatg acgaaaataa cgaactgcgc ggcagcttct
     7501 acctcgatct gtatgcccgt gaaaacaagc gcggcggggc gtggatggat gactgcgtag
     7561 gccagatgcg taaagctgat ggttctctgc aaaaaccggt cgcgtatttg acttgtaact
     7621 tcaaccgccc ggtaaatggt aaaccggcgc tgttcactca cgacgaagtg atcaccctgt
     7681 tccacgagtt cggtcacggc ctgcaccata tgctgacccg catcgaaacc gctggtgttt
     7741 ccggtatcag cggtgtgccg tgggatgcgg tcgaactgcc gagtcagttt atggaaaact
     7801 ggtgctggga gccggaggcg ctggcgttta tctctggtca ctatgaaacc ggcgaaccgc
     7861 tgccgaaaga gttgctggat aaaatgctgg cggcgaagaa ctaccaggcg gcgctgttta
     7921 ttctgcgtca gctggagttc ggcctgtttg atttccgcct tcatgccgag ttccgcccgg
     7981 atcagggggc aaaaatcctc gaaactctgg cagaaatcaa gaaactggtt gccgtggtgc
     8041 catctccgtc ctggggccgt ttcccgcacg ctttcagcca tattttcgcc ggtggttatg
     8101 cggcaggtta ctacagctac ctgtgggctg acgtactggc ggcagatgct ttctcgcgct
     8161 ttgaggaaga gggcattttc aaccgtgaaa ccgggcagtc gttcctcgac aacattctga
     8221 gccgtggcgg ttcagaagag ccgatggatc tgttcaaacg cttccgtggt cgtgaaccgc
     8281 agctggatgc gatgctggag cattacggca ttaagggctg atcattcagt gaaaatctgc
     8341 ttaattgatg aaacaggcgc cggagacggc gccttatctg ttctggcggc ccgctggggg
     8401 ctggagcacg atgaagacaa cctgatggcg ctggtgttaa cgccggaaca tctggaattg
     8461 cgcaagcgtg atgagccaaa acttggcggc atctttgttg attttgttgg cggagcgatg
     8521 gcgcaccgac gcaaattcgg cggtggtcgc ggtgaggcgg tggcgaaagc ggtgggcatt
     8581 aaaggcgatt atttgccgga tgtggtggat gccactgcag gactggggcg cgatgccttt
     8641 gtgctggctt cagtcggttg ccgcgtgcgg atgctggagc gtaatccagt tgttgccgcg
     8701 ctgctcgacg acggcctggc gcgtggttat gcggatgcgg aaatcggcgg ctggttgcag
     8761 gagcgtttgc agttaattca cgcctccagc ctgacggcgc taaccgatat taccccacgc
     8821 ccgcaggtgg tctatctcga cccgatgttc ccgcataagc agaaaagcgc gctggtgaaa
     8881 aaagagatgc gtgtctttca gtcgctggtg gggccggatc ttgatgccga tggattgctg
     8941 gagcctgcac gcttgctggc gaccaaacgc gtggtagtaa agcgcccgga ctacgcgcca
     9001 ccgctagcga atgtcgccac gccaaacgcg gttgtgacta aagggcatcg ctttgatatc
     9061 tatgcaggta cgcccgtgta aaagtaaagc agggtagcga taacgctacc ctgctttctg
     9121 caagaataat taatggcttt ccggcgtcgc aatcatgcgt ttcagccacg gcaccatcaa
     9181 cagcatcact actgcaacgc ccagcgtaac caggccaatc ttaccaaaca cgttggtata
     9241 gacgggtaac gtctcaagcg gatcggtaat gttgtccggc actgcggtaa atgttgccac
     9301 atagccgccc agcaagaatg cggcagcctg cgtcaggaac cacatcccga gaataaagcc
     9361 catcaaatgc tgcggcacca gggcagcaat catcgccagg ccaagggcgc taataaacaa
     9421 ttcacccagg ctctggaata aatacaccag cacgataaac catggcgatg tcagcccttg
     9481 tgcatccgca aaccacattc ccgcagctgc cgccgtcaaa aagcccagtg agcacataaa
     9541 catgccgaga gtaaatttca tcggcatcga gaggtctttg cctttgttac ccagatgcgt
     9601 gtaaatgcct gccagtattg ggctggcgag taccacccag aacgggttaa gcgcctggaa
     9661 gctgaccggg tttatggaaa agccgagaat ttcatgatgc acgttgttga tggcaaagaa
     9721 gttcagcgac gttggcatct gggcgtagag aatgtaaaac accaccgctt cgagcatcag
     9781 gacaaaggcg acaaacattt tattgcgccc ggttttatcc agcttgaatg cctgacgaaa
     9841 gaagatgatg gtgacgacga tggagagaac aatcagcacc agattggcga cttctacgtt
     9901 gtgcatcagc catgcacata cgaagatcat caccacgctg ccaagtaata cgtacaacag
     9961 tttgctgaag ctcatcggct tgaagtcggg ttcagaacca atgtctttca ccattccacg
    10021 acaggcgatg taaaccagta atgcgataat taaccccgcc ccgcacaggt tgtaggtgac
    10081 tgaataaccg tatctatcag cgatcacagg ggccagcgat aacgctatca acgagccgat
    10141 attgatcgac atatagaaca gggtgaatgc gccatcaagc cgcggatctt tcggcggata
    10201 gcacttcgaa agcaagctgg ctgggttagc tttaaacagg ccgttaccga cagcgatagt
    10261 ccccagggcg atgaaaatca ggtcaggctt aagtagcgac atgccggtca tgaagtagcc
    10321 aatcgccagc acaagtgctc cgagaacaat ggtgcgtttg gtccccagca ggtggtcgcc
    10381 gacatagccg ccaatggaaa tgaggccata gaccagcgca gcaaaagcac caaaagtgac
    10441 aaaagcctgc tcttgcgaga atccaagctg tttaacgaag aaaaccgcca gtacgccctg
    10501 cacgccgtag tagccgaatc gctcccataa ctcgacaaaa aagatcatga aaaatgggcg
    10561 aggttgctgc agcatcccca tgggtgttgt tgtattcata tttattaacc ttccaatacc
    10621 atcctgaaaa gtatgaacgc gtaagggata acgcgcgaaa aagtttacat ctgtcagaga
    10681 ttcttaatat tgatgcggtg aatagattaa tacactatta caacagaaaa taaccagctg
    10741 atcacactaa tgatggaaaa tatgatagtg ttaaatgttg tattggcagg agattttgat
    10801 tgcgtacgca gatcgtaggc cagataaggg gtttacgctg catcaggtaa caaggcgcgc
    10861 caaaaagcaa aacgcccgca tatgcgggcg ttcaggaaca cgtcgtgggg ggatgattat
    10921 tcttcttcgt cgcgcagcgg aacaatcagc atatcaacgt gaacggtgtt gatcagctga
    10981 cgtgcggaag acattagttt gctccagaag tcctggtggt gaccacaaac caccagatcc
    11041 atatcgtatt tcttgattgc atcgaccaga acctggccca ggtcgccgct gccgctcagg
    11101 gtttcagtga ttgggtagcc tgcattagtg gaaagctcgg tcagcgcatg atgtgtctct
    11161 tcagagatgc gtttctgcat atcacccaga ttcacatcaa taagcccggt gtataggtca
    11221 gagtagttta catctacgtg gatcagagaa actttcgcat tgtaggggcg agccatagag
    11281 actgctttct ctaccagaac tttgctttcc ggggagagat cgaccgcgat gagaatgtgt
    11341 ttataagcca tagtgttact ccttccataa agttgtcgat gactggccag ctagcgtttc
    11401 ttgtgccgtt acgtcacccg cgtcctgcga actacgtgcg cggggctaac ccagccgtaa
    11461 agattcagtg ttaagaccat ccttacctta tagcgacccg gatgatccgt caatccgcct
    11521 tgcttacccg ttagataaac aaattttcga catgaatata ttgatagtgg ttaaccttct
    11581 ggaaaaaaaa caacctgatc tcctacacta tctatagagc cgctcggatg ttggtcacca
    11641 caaaagcggt ttctccagga cctggtgtta actgattggc agcgtatcgg gagcggtagc
    11701 cccggggtgg aactccgcgg gcaggtcgcc ggggaggagg tatgataagc accgtcgcat
    11761 tattttgggc tttatgtgtc gtttgcattg ttaacatggc gcgctatttc tcatcactac
    11821 gcgcgttgtt agtggtactg cgtaactgcg atccattgct ctatcaatat gttgatggag
    11881 ggggcttttt tacctcacat ggccaaccca acaaacaggt gcgtctcgtt tggtatatct
    11941 atgcccaacg ttatcgcgat catcacgatg atgaatttat tcgccgctgt gagcgggtgc
    12001 gtcggcagtt tattctgacc agcgcattgt gtggtctggt ggtggtcagc ctgattgcat
    12061 tgatgatttg gcattaagca cttcagcaat aataacgcat aaaaaaagcg ggccagtaaa
    12121 ctgacccgct cgagactgat tacaaacgca aaattgcctg atgcgctacg cttatcaggc
    12181 ctacataatc tctgcaatct attgaatttg catgtttttg taggccggat aaggcgttca
    12241 cgccgcatcc ggcatgaaca aagcgcacta tgtcacaatc tgaagcgggc cagctgaccc
    12301 gctcgttttg gtgcgtacga ttacaggaac tgcaaggaga gccagtacag cccgccggaa
    12361 agcagtaccg cagccggaag ggtaaacacc caggccatca gaatactggt tacggtttta
    12421 cgctgtaagc cgccgccatc taccaccatc gtccccgcga cagaagagga gagtacgtga
    12481 gtagtggaaa ccggcatccc ggtataactc gccaggccga tagacactgc cgccgtcatc
    12541 tgggcagaca tcccctgagc gtaggtcatg cctttcttac cgattttctc accgatagtc
    12601 gttgccacac ggcgccagcc aatcatcgta ccgataccta acgccagcgc gaccgccatg
    12661 atgatccaca ccggtgcata ctcgatggtg ctaagcatgt cggacttcag tttcttcaac
    12721 aggcgctgat cgtcagcact cacgccaggc atcttcacca ctttgtcgat agtgtcagaa
    12781 acgcacagca taatgcggcg catctggcta cgttgatcaa gcgacagctt gtcgtagctt
    12841 tccacatcgg tggtcaacat acctttcagg cggttgagcg cgttaatggt attcgacgga
    12901 tggcagtgga actccgcagg ttgcgttgcg ccagcttccg gagccggtac taactgatca
    12961 gcaccggtag cctgtttgag cagcgcagga tgctgctcaa agtaagcttc gacgttgttg
    13021 atggcatcac gggtacgggt gatttcgtag ccagtggcat tcatgttcac cacgaagcct
    13081 gctggcgcga cgccaatcaa taccaacata accagaccaa tgcctttctg accatcgttc
    13141 gcgccgtgcg aaaacgccac gccgatagcg gaaaggatca gcgcgatacg tgtccagaac
    13201 ggcggctttt tcttgccgtc tttcttttca cgctccgctg gggtcaggtg gatacgggcg
    13261 cgtttcttgg tgccgctcca gtagcgacgc agcaagaaaa tcagaccgcc agcaaacacc
    13321 aggccgacaa taggggaaac gatcagagaa ccgaaaatac ttaatacttt cgggatattg
    13381 agtgcatcca ccactgacgt cccggtcatc aacgcattgg ttaaaccaat cccgatgatc
    13441 gcgccaatca gcgtatgaga gctggatgca ggtaaaccaa agtaccaggt acccaggttc
    13501 cagataatcg ccgccagcaa catagagaac accatggcaa ggccatgaga cgatcccata
    13561 ttaagcagca gatccgtcgg cagcatatgc acaatggcat aggcaacact cagaccaccc
    13621 agcaaaacac ccaaaaagtt gaacaccgcc gccataacca cggcgagctg agaacgcatc
    13681 gcgcgggtat agataacggt tgccacggcg ttggctgtgt catggaaacc attgatggct
    13741 tcgtagaaca gcacaaaagc cagtgcaagc aataataaca gcccggtatg caaatccagg
    13801 ccagcaaaca aatgtagcat aggacgttac gccattttga ggacatgaac gcggcgcatt
    13861 atcagtgact ttcgcggcgc gggcaaagtg aaatatagac tttttttgat ttgcctcctg
    13921 tatggatttc actcaaaaaa taattatctt atataattca ggcaaatact tccttttagt
    13981 aatattgatg ctggtgcgac cactgaggaa tctttacaat tcacgcccgt tttttctaag
    14041 aggagcgcaa cgtggaaagg tttgatgcca ttattatagg cgctggtgcg gcgggtatgt
    14101 tctgttctgc gctggcaggt caggcaggac gccgggttct gctgatcgat aatggtaaaa
    14161 aaccagggcg caaaatcctt atgtctggcg gtgggcgctg caactttacc aacctttatg
    14221 tcgaaccagg cgcttatctg agccagaatc cgcatttttg taagtctgca ctcgcacgtt
    14281 ttacccagtg ggatttcatt gatctggtca ataaacacgg catcgcatgg cacgagaaaa
    14341 cgttagggca actcttctgc gatgactccg cgcagcagat tgtcgacatg ctggtggatg
    14401 agtgcgagaa gggcaatgtg accttcagat tgcgtagcga agtgctgagt gtggcggagg
    14461 atgaaacagg cttcacgctt gatctgaacg gcatgactgt cggttgcgaa aagctggtca
    14521 tggcgaccgg tgggctgtca atgccggggc tgggcgcgtc gccgtttggt tataagattg
    14581 ccgaacaatt tggcctcaac gtgctgccga cccgtgcggg tctggtgcca ttcactctgc
    14641 ataaaccgtt gctcgaagag ttacaggtgc tggcgggcgt ggcggtgcct tccgtgatta
    14701 ccgctgaaaa cggcatcgtt ttccgtgaga acttactctt cacccatcgc ggcttgtctg
    14761 gaccggcggt gttgcagatt tccagctact ggcaaccggg tgaatttgtc agtatcaatc
    14821 tgctaccgga tgtggatctc gaaaccttcc tgaatgagca gcgtaacgca catccgaatc
    14881 agagcctgaa aaacacactg gcggttcatc taccgaagcg gttggttgaa cgcttacagc
    14941 aactcgggca aatcccgaat gtttcgctaa aacagctcaa cgtgcgtgac caacaggcac
    15001 tgattagcac attgaccgac tggcgtgtac aacccaacgg cactgaaggc tatcgcactg
    15061 ccgaagtgac gctcggcggc gtggacacca acgaactctc ttcacggacg atggaagcgc
    15121 gcaaagtgcc tgggctgtac ttcatcggcg aagtgatgga cgttaccggc tggctggggg
    15181 gctataactt ccagtgggcg tggtcgagtg cgtgggcttg tgcgcaggat ttgattgcag
    15241 cgaagtcgtc ctgacctgtt acctaaagat gaaaattatt agagccgcat taaaaatgag
    15301 ctaattttga tagtggctat cttgtgatta ttttctaatg agcccgtgaa ctgaaaccct
    15361 ccaggcttaa tataaggtgg aaggaaaggt gattgaaaac tcacccagtg gccagatctt
    15421 tataaaatat gaacaggctg aaaaagacag taactttgtt gtttttttat ccttaaccac
    15481 tatgcattaa tgctgcgtta tttcatgatg cctaagaaaa accagcgtta cgcaaatggt
    15541 caacgctggt tttatccggt acgttgcaat tattttttag cagaacccgc ttctaatatt
    15601 gaaacgattg agaacaacgt aaagcatacc gctcccaaac cgaccagaac tcgcgaagga
    15661 ataaaatatc ccatgtcggt ctgcgccatt tccgcaagaa atgatgccag gaaaaggcaa
    15721 aacaggcagg tgaagacggg tatcatcggt attctgttgg ctaacgaaca ggtacgtctc
    15781 cagaccagtg ccagtagcca gacttttgag aatatgctgt aacagatcat tccgaggcaa
    15841 ataagaataa tgccgggagc cagtcgggcg cttgcatcgg aactgactaa gacgtatatc
    15901 ccctgcagta ccgtgattga gccgagaaaa ataacccaat aacaccacag ccagtgttct
    15961 ttagttgaaa acgtattacg tgtttgatgg acaattgtgg caacaaggcc aattagacag
    16021 gcacagattg cggttaaccc aagcaatacg tgacccgcga cataatgcgg agttatgtca
    16081 gcactacgta acagcgtaat agaccaaatg aaaccgagca gggtgagtaa aacgggaacg
    16141 gcaatgaggc aattaccgat taatgaagaa taagcctgta ccggcgttcc atcgctcttg
    16201 ctccccgctg catttttggg aatgagcaga aagtgaccgg atgacgctgc caccgtcgat
    16261 acacaggcgg caatcatacc aacgccgaaa ataacatggc cggcgacaaa ctcgtctgcc
    16321 atcacatcat tgcctgctaa cagtgcccaa ccccatatca tggtgataat tgaccccgca
    16381 tagccaataa tggggaacaa tgtattgtaa aacgtattaa cgccgcgcgt gagctgcgaa
    16441 atgataataa atgcggtagt gaataatgcc aggcatatgg ccgccagaga aatcagcaca
    16501 tgacccgcga cgaaatactc gctttgccct gaactttcca gcacaaagcc acccgatata
    16561 tatgttcact ttaaactcct caggtatccc tgctaataat gaaatgtgca ttaatgataa
    16621 tttcagcctg ttaatataac gacgagatat cattaaagac aaatgcatag cataactata
    16681 gcggggttta ctgtgccgag tgccattttt taccggttaa aaggccatga taaataaata
    16741 tttatcctaa gattaattct tattctgttg ttttttttac aattatcttc cagggatgga
    16801 ttgttttcca ttatttagta aaagaccgga tagtctcggt gcttaaaatc atcgaacata
    16861 taagtattat tgctttgtat catcgtaaat atgtctttca gacatgtatt gtttattttg
    16921 ttactaacaa gtaaaaatat tacaggttat ttatgaaagt atttcacaat atatttaagc
    16981 acttctcttc aaaccatcaa gataagcatt cagataaagt taacagtcat caacaccatg
    17041 gcaaggttga taaaaaatat cgatcaaaca cagttgaagt taatatgctg aatgactttt
    17101 cgcaaataga taacgattta ggcctgaata tcatctattt cctgcaacat ggtcactgga
    17161 gggtaaatga acatagtctt cagatggaga aaatatggtt ttacaatagt gagccatcta
    17221 tagatattca agaatataat agtttcgccg ataatactac taacacgttt atctttacaa
    17281 ttataccaga caataaccat gtgataaaat tatcgtctcc cattacagtt aagattgagt
    17341 gtaaggatgg ttattatttt attaatttct ccggagataa atcagacata atttataaat
    17401 cagacataac ttataaagtt aatgggcttt ctatcatacc tgaaaatttc tttactcttc
    17461 ttagcggtaa ttttaaagct gactggcgtt gggatgtctc taaagagacg tttcctaagg
    17521 ataagtttga tagctatgtt aaatctgtgt tttctaaaat agacttttat aaacaatgtg
    17581 gtgtgattaa cccacaaaat gccaacacgg cttattttgg cgatacagat ggaagagtag
    17641 gcgctgtact ttatgcattg ctcgtttcag ggcatatagg catcagggaa aagggttgga
    17701 gtttattatg tgaattatta aaacacgaaa acatggcttc atctgcatac atacataaaa
    17761 ataataaatt gctctacaat ttattaaata cgagagacat gatattgaat gagttgcatc
    17821 aacatgtttt tttaaaggac gatgcgataa ccccttgtat cttccttggg gatcatacag
    17881 gcgatagatt tagtactatt tttggtgata aatatatcct tactttgcta aattctatgc
    17941 gaaatatgga tggtaataaa gatagccgta ttaataaaaa tgtcatcgtt ctggctggaa
    18001 atcatgaaat caattttaat ggtaactata ctgcacgact agaaaattat aaactctctg
    18061 ccggtgatac gtataactta attaaaacgc tcgatgtttg tcattatgac tcagaaacga
    18121 aagttttaac cagtcatcac ggaataataa gaaatgaaga aaatgaatgt tattgtctcg
    18181 gtgctttgca ggttccattt aatcagatga aagacccaat tgatcctgag gaattagcaa
    18241 atatatttaa taaaaaacat aaacaacaca tggatgatcg tcttatccat ctaattagaa
    18301 gcaatgctgt agcatccaca cctgtatatg aaaattattt taataacact actgctttca
    18361 gaccaaaacc tgaagatatc ttcaaatgtg gacagacgct caaaaaaact aaacagaaat
    18421 atggccatta tggattaggg gtagatcagc atcaaaaaat tgataattat accatggggc
    18481 taaattcctg gaagatagca cctaacgaac gtggtgataa aaaaggtgtc ccagggctta
    18541 gttgttttca acctcaataa aacttcatga gtgctgtggt atctcttttt aattttgcca
    18601 gtatttcata ggttttctta atcgattgta gaggcatgtt ctgatgacgc ctctacaatc
    18661 tttagggact ttctctgcta tgagataaga aatttcatat tatgctttaa ggctgaatga
    18721 tttgtgcaaa atataattat tttcccatcg aacatgaggt gtatgtcata agattaaatg
    18781 ttaactatgt attcctcgcg gtaaataata tgaggtaaaa gatgaaaatt ggaactgtgg
    18841 caggcactaa cgacagtaca acgacaatca caacaaatga tatggtgcaa gagcatgtta
    18901 ccaacttcac taaagaactg tttgggtata ttgcaaatgg tataggtgat gatatatcaa
    18961 gtattgccag gacaatgctc ggtgaggttg tggaaaaaat agatgattgg caaatagaac
    19021 ggttccaaca atctattcaa gatgacaaaa tttcatttac catacaaacg aatcattcag
    19081 aaaaatatag tatgctatcg ggcatgcgcg ctcatattct acgtaggaat aactgttatc
    19141 agtttattgt gacaattaat agtaaaaatt acggttgccc tcttgataat accgatatta
    19201 attggtgcag cattgtttat ttacttaata atatgacagt aaatgataat gctaatgatg
    19261 ttgcagttac tgagtcgtac aaacccatct ggaactggaa aatatcgcaa tacaatgtat
    19321 ttgatattaa attcgaaaca atgataaaac cccagttcgc tgacagaacc tatttctcca
    19381 attgttcacc tgttgatccc acaagtacca gaccgaccta ttttggcgat accgatggct
    19441 cagtcggtgc agttttgtac gcgctttttg caacgggaca tttgggaatc atggctgaag
    19501 gagaaaattt ccttagccag ttgcttaata tagaagatga agttctcaac gtattgctaa
    19561 gggagaactt taatgagcaa cttgacacta atgttaatat aataatcagt atcctgaaca
    19621 gaagagacat tgttttagaa agtcttcagc cttatttggt tattaataaa gacgcagtca
    19681 cgccatgtac gtttctgggc gaccagactg gcgatcgatt tagtaatatc tgtggcgata
    19741 aatttatcat cgatttatta aaacgcataa tgagtatcaa tgaaaacgtt catgtattag
    19801 ctggaaatca tgaaacgaat tgcaatggta actatatgca gaacttcacg cgcatgaaac
    19861 cacttgatga ggatacctac tctgggataa aagattatcc tgtatgtttc tatgacccca
    19921 aatacaaaat aatggcaaat catcatggga taacttttga tgatcagcga aaacgttaca
    19981 ttataggacc gataacagtc tctagtgatg aaatgaccaa tgctctcgat ccggtcgaat
    20041 tagcggcaat tatcaataaa aagcaccatg ctgtaattaa tggcaaaaag tttaaaaata
    20101 gccgggctat ctcttgtcga tcctttaatc gttatttttc tgtttcgaca gattacagac
    20161 ctaaacttga agcactatta gcatgctcac aaatgttagg tataaatcaa gttgttgcac
    20221 ataacggtaa tggtggaaga gaatgtatag gtgaaacggg gacagtttta ggtcttaacg
    20281 cccgtgacag taaacatgct ggaagaatgt tcagcatgca taattgccaa attaaccctg
    20341 gtgcagggcc tgagataacc actccctgga agtcttacca gcatgaaaaa aacagaaatg
    20401 gacttatgcc tttgatcaga aggcgcacaa tgttacagct ctgaaccata gacaacatga
    20461 atattaatgt tactatctgg ttgagactgt tgataaaacg taaaaaggaa tgctctgttc
    20521 ctgcaactga acaggcgtga aacgctatcc aacaggatgg ataccgtttt gtcagccagt
    20581 tttatagatc acacttatga gtatgcgtgt ttcatttacg gccaggaatg ctgatgatga
    20641 caacattact acccgttttc actaagcctt ccccgttggc gctcaatgct ctgcgcgctg
    20701 gtcgtatttg tcgtttcctt cttatcccgg atggaagaat ccgctgaacg tcgttcggcg
    20761 gcagttgtaa cacgcgtcgt ttgcttgcag ggattatggc gggtcgatta tggataagag
    20821 taagcgccat ctggcgtggt gggttgtcgg gttactggtg gtggctgccg tcgtggcgtg
    20881 gtggctgttg cgcccggcgg gtgtgccgga aggctttgct gtcagcaatg ggcgcattga
    20941 agcgacggaa gtggatatcg ccagcaaaat tgccgggcgt atcgacacca ttctggtgaa
    21001 agaaggccag tttgttcgcg aaggcgaagt gctggcgaag atggatactc gcgtgttgca
    21061 ggagcagcga ctggaagcca tcgcgcaaat caaagaagca caaagtgccg ttgctgccgc
    21121 gcaggctttg ctggagcaac ggcaaagcga aactcgtgcc gcgcagtcgc tggttaatca
    21181 acgccagtcc gaactggact ccgtggctaa acgtcatacg cgttcccgct cgctcgccca
    21241 acgaggggct atttctgcgc aacagctgga tgacgatcgc gccgccgctg agagcgcccg
    21301 agctgcgctg gaatcggcaa aagctcaggt atcggcttct aaagcggcta tagaagcggc
    21361 ccgtaccaat atcattcagg cccaaactcg cgtcgaagcc gcgcaagcca ctgaacggcg
    21421 tattgccgca gatatcgatg acagtgaact gaaagccccg cgtgacggac gcgtgcagta
    21481 tcgggttgct gagccagggg aagtgctggc ggcaggcggt cgggtgctga atatggtcga
    21541 tctcagcgac gtctatatga ctttcttcct gccaaccgaa caggcgggca cgctgaaact
    21601 tggcggtgaa gcccggctga tcctcgatgc cgcgccagat ctgcgtattc cggcgaccat
    21661 cagttttgtc gccagtgtcg cccagttcac gccaaaaacc gtcgaaacca gcgatgaacg
    21721 gctgaaactg atgttccgcg tcaaagcgcg tatcccaccg gaattactcc agcagcatct
    21781 ggaatatgtc aaaaccggtt taccgggcgt agcgtgggtg cgggtgaatg aagaactttc
    21841 gtggcctgac gacctcgtgg tgaggttgcc gcaatgacgc atctggaact ggttcccgtc
    21901 ccgcctgtcg cgcaactggc gggcgtgagc cagcattatg gaaaaaacgt tgcgctgaac
    21961 aatattactc tcgatattcc ggcccgctgt atggtcgggc tgattggtcc ggatggcgtc
    22021 gggaagtcga gcttattgtc gttgatttcc ggtgcccgcg tcattgagca gggcaatgtg
    22081 atggtgctgg gcggcgatat gcgcgacccg aggcatcgcc gcgacgtctg cccgcgcatc
    22141 gcctggatgc cgcaggggct gggcaaaaac ctctaccaca ccttgtcggt gtatgaaaac
    22201 gtcgattttt tcgctcgcct gttcggtcac gacaaagcgg agcgggaagt gcgaattaat
    22261 gagctgctga ccagcacggg gttagcaccg tttcgcgatc gtccggcagg gaaactctcc
    22321 ggcgggatga agcaaaaact tgggctgtgc tgcgcgttaa tccacgaccc ggaactgttg
    22381 atccttgatg agccaacaac gggggttgac ccgctctccc gctcccagtt ctgggatctg
    22441 atcgacagta ttcgccagcg gcagagcaat atgagcgtgc tggtcgccac cgcctatatg
    22501 gaagaggccg aacgcttcga ctggctggta gcgatgaatg ccggagaagt gctggcaact
    22561 ggcagcgccg aagagctacg gcagcaaacg caaagcgcta cgctggaaga agcatttata
    22621 aatctgttac cgcaagcgca acgccaggcg catcaggcgg tagtgatccc accgtatcaa
    22681 cctgaaaacg cagagattgc catcgaagcg cgcgatctga ccatgcgttt tggttccttc
    22741 gttgccgttg atcacgttaa tttccgcatt ccacgcgggg agatttttgg ttttcttggt
    22801 tcgaacggct gcggtaaatc caccaccatg aaaatgctca ccggactgct gcccgccagc
    22861 gaaggtgagg cgtggctgtt cgggcaaccg gttgatccaa aagatatcga tacccgccgt
    22921 cgggtgggct atatgtcgca ggcgttttcg ctctataacg aactcaccgt gcggcaaaac
    22981 cttgagttac atgcccgttt gtttcacatc ccggaagcgg aaattcccgc aagagtggct
    23041 gaaatgagcg agcgttttaa gctcaacgac gttgaagatg ttctgccgga gtcattgccg
    23101 ctcggcattc gccagcggct ttcgctggcg gtggcggtga ttcatcgccc ggagatgtta
    23161 atcctcgatg agcctacttc tggtgtcgat ccggtggcga gggatatgtt ctggcagttg
    23221 atggtcgatc tctcgcgcca ggacaaagtg actatcttca tctctaccca ctttatgaac
    23281 gaagcggaac gttgcgaccg catctcactg atgcacgccg gaaaagtgct tgccagcggt
    23341 acaccgcagg aactggttga gaaacgcgga gccgccagtc tggaagaggc atttatcgcc
    23401 tatttgcagg aagcggcagg gcagagcaac gaagccgaag cgccgcccgt ggtacacgac
    23461 accacccacg cgccgcgtca gggatttagc ctgcgccgtc tgtttagcta cagccgccgc
    23521 gaagcgctgg aactgcgacg cgatccagta cgttcgacgc tggcgctgat gggaacggtg
    23581 atcctgatgc tgataatggg ttacggcatc agtatggatg tggaaaacct gcgctttgcg
    23641 gtgctcgacc gcgaccagac cgtcagtagc caggcgtgga cactcaacct ctccggttcc
    23701 cgttacttta tcgaacagcc gccgctcact agttatgacg agcttgatcg tcggatgcgt
    23761 gcgggcgata tcacggtggc gattgagatc ccgcccaatt tcgggcgcga tatcgcgcgt
    23821 ggtacgcctg tggaactcgg cgtctggatc gacggagcga tgcccagtcg tgccgaaacg
    23881 gtaaaaggtt acgtgcaggc aatgcaccag agttggcttc aggatgtggc gagccgacaa
    23941 tcgacacccg ccagccaaag cgggctgatg aacattgaga cgcgctatcg ctataacccg
    24001 gacgtaaaaa gcctgccagc gattgttccg gcggtgatcc cgcttctgct gatgatgatc
    24061 ccgtcaatgc taagcgccct tagcgtggtg cgggaaaaag agctagggtc gattatcaac
    24121 ctttacgtga cccccaccac ccgtagcgaa tttttgcttg gcaaacagct gccgtacatc
    24181 gcgctgggga tgctgaactt tttcctgctc tgcggcctgt cggtgtttgt gtttggcgta
    24241 ccgcataaag gcagtttcct gacgctcacc ctggcggcgc tgctgtatat catcattgcc
    24301 accggaatgg ggctgctgat ctccaccttt atgaaaagcc agattgccgc cattttcgga
    24361 acggcgatta tcacgttgat cccggcgaca cagttttccg ggatgatcga tccggtagct
    24421 tcgctggaag ggcctggacg ttggatcggc gaggtttacc cgaccagtca ttttctgact
    24481 atcgcccgcg gaacgttctc gaaagcgctg gatctgactg atttgtggca actttttatc
    24541 ccgttgctga tagccatccc gctggtgatg ggcttaagca tcctgctgct gaaaaaacag
    24601 gagggatgat gcgccattta cgcaatattt ttaatctggg tatcaaagag ttgcgcagtc
    24661 tgctcggtga taaagcgatg ctgacgctga ttgtcttctc gtttacggtg tcggtgtatt
    24721 cgtcagcgac cgttacgcca ggatcgttga acctcgcgcc gatcgccatt gccgatatgg
    24781 atcaatcgca gttatcgaac cggatcgtta acagcttcta tcgtccgtgg tttttgccac
    24841 cggagatgat caccgccgat gagatggatg ccggactgga cgccggacgc tataccttcg
    24901 cgataaatat tccgcctaat tttcagcgtg atgtcctcgc cggacgccag ccggatattc
    24961 aggtgaacgt cgatgccacg cgcatgagcc aggcatttac cggcaatggg tatatccaga
    25021 atattatcaa cggtgaagtg aacagctttg tcgcgcgcta ccgtgataac agcgaaccgt
    25081 tggtatcgct ggaaacccgg atgcgcttta acccgaacct cgatcccgcg tggtttggcg
    25141 gggtgatggc gatcatcaac aacattacca tgctggcgat tgtattgacc ggatcggcgc
    25201 tgatccgcga gcgtgaacac ggcacggtgg aacacttact ggtgatgccg ataacgccgt
    25261 ttgagatcat gatggcgaag atctggtcga tggggctggt ggtgctggtg gtatcgggat
    25321 tatcgctggt gctgatggcg aaaggtgtac tgggcgtacc gattgaaggc tcgatcccgc
    25381 tgtttatgct gggcgtggcg ctcagtctgt ttgccaccac gtcaatcggc atttttatgg
    25441 ggacgatagc gcgttcaatg ccgcaactgg ggctgctggt gattctggtg ctgctgccgc
    25501 tgcaaatgct ttccggtgga tccacgccgc gcgaaagtat gccgcagatg gtgcaggaca
    25561 ttatgctgac catgccgacg acacactttg ttagcctcgc gcaggccatt ctctaccggg
    25621 gtgccggatt cgaaatcgtc tggccgcagt tcctgacgct gatggcaatt ggcggcgcgt
    25681 ttttcaccat tgcgctgctg cgattcagga agacgattgg gacaatggcg taaggaaggg
    25741 aaacttgact tcatactgtg atgtcatact attgatgtca taaagtgaag tcataaggac
    25801 atgccatgag aaccaaggta caggctttac ggaagaaaaa aaaatacatt ggatcaaata
    25861 tttaaaactc ctgttcctca agggatcaag tggtctgata tagagtcact ggttaaagca
    25921 ttaggcggag aaattaagga aggaagaggt tcgcgttgta agttcatact aaatatgagc
    25981 gttgcgtgtt tccatcggcc tcatccgtcg ccagataccg ataaaggcgc tgtagaaagc
    26041 gtgcgtgact ggttactaag tataggggta aaaccatgat caaactgaag acgccaaact
    26101 ctatggagat tgccgggcaa ccagctgtta ttacctacgt gccagaactg aatgcatttc
    26161 gtggcaaatt cctgggcttg tcaggttatt gtgattttgt ctctgacagc attcaggggc
    26221 tgcaaaaaga aggggaactt tccctgcgag agtatcttga ggattgtaaa gctgcgggta
    26281 ttgagccgta tgcgcgtaca gaaaagatta agacctttac gttacgctat ccagaatcgc
    26341 tgagtgaacg ccttaataat gcggctgcgc agcaacaagt ctctgttaat acctatatta
    26401 ttgaaacact gaatgagcgt ttgaatcatt tataaatttt agcggcgtac attacgcaga
    26461 atatctgcct ttacaggatc tatctatgca actgaactta attttataaa ttaagttcag
    26521 ttaatatctt ttctttcctc gtttcagaaa taactcaatc ttccttcggc aagcactgca
    26581 aatgcccatg ccgcacgccg cgctgggcga taacgtcatc ggcaaaatgc tgcacgtcgc
    26641 ccatatcacc tttcagcacg gcgatttcca gacagtcgtc gtgattgata tgcacatgca
    26701 gcgtggcgac ggagagatcg tggtgatggt gctgggtgga gacaatgcgg ctggctaagt
    26761 cgcgtttttc gtgttcatac acataggaga gcaccgcgaa accttgcgtg ccgtgctgct
    26821 gagtggcctc ctgggccagg gcgctacgca gaatatcgcg gatagcttcg gaacggttgt
    26881 tataaccacg acgctggctc aggctgtcca gcgtctccag taaatcgtca tcaagcgtga
    26941 tggtgactcg ttgcatttgc gttaaacctt ttctgtggtg cgacggcgca cggggaatgc
    27001 gggtaatacc gcgttttgta gcacacgtcc ggcgtcagag gaaaaggtta atttatctcc
    27061 caccacctgg gtttcgacga tttgtccgtt gtccatcacc atcacccgct gacagaagcg
    27121 ttccaccagg cgtaagtcgt gggtgatgaa caggcaggcg gtgccaaact gttgttgtag
    27181 ctttttcagc aggcgaatga cgcccgcctg taacacgaga tcaaggttag aaacggcttc
    27241 atccagaatc agtagtttcg gttcgaccgc cagcgcgcga gccaggcaga cgcgctggag
    27301 ctggccgccg cttaactgcg gtgggcgttt gtcgagaacg ctgtcatcga gatcgaccgc
    27361 cttcagcatt tcgctggcgc gcgccagttg ttcggatttt ttcagtgata gcaggtggcg
    27421 catcggttca cgcaggatct cgcgcacggt tttgcgcgga ttcacggcgc tgatggagtc
    27481 ctgaaatacc atctgaatat cgcggcggaa cgctttacgc tgggcgcgat tgagtttcgc
    27541 cagcggttcg ccacgccagc taatattccc ctgcgcgggc gattctaaac ccaccagcaa
    27601 ccgcgcgagg gtacttttcc cgcagccgct gcgccccagc agggcgacgg tttcgccgct
    27661 tttcagggtc agggaaacgt tattcagcac cgcctgatgt tgatgttttc cgttaaatcc
    27721 accgtgggca tagtgatggg aaaggccgga gacgttaagt aaagtcatga tgccagctcc
    27781 ataccgtaga gggcgagatg agcggaaacc aggctgcgcg ttaccgcatg tttgggggcg
    27841 ttaaacagcg tttctacatc gccctgttcg acaattttac cttgtgacat caccgccacg
    27901 tcatccgcca gacgcgccac aacgcccata tcatgggtga ccagcagcat tcccggcgct
    27961 tgtttttgca taatgctttc cagcagatcg aggatgcgcg cctgtgctac cacgtcgagg
    28021 tcggtggtcg gttcatcggc gatgataaac ggtgattcac acagcaccgc catcgcaatc
    28081 atcatgcgct gcaacatgcc gccgctcatc tcgaacgggt acagcttcag cacgcgcgcg
    28141 gcgttttcca gccccaccgc ttctatggca gcggtaagcg tagcgtcatc ggcgggtttc
    28201 cctaacgcca ggcaggtttc ccgcgcgtgg gtgtgcatgg tgtgtagcgg attaaaggcg
    28261 ctgcgcgggt tctgcatgat ggtggcaatt ttgatgccgc gcagggcgca gggataaacc
    28321 gctttgccat cggctaaaat ttcccccgcc gtctggcgaa cgccagcggg caaaatgccc
    28381 agcgtcgcgg cgcaggttaa tgatttcccg ctgccgctac cgccgactaa cgccagcacg
    28441 cgcccgcgtt gcagggttaa cgatacaccg tgtaccagcg gctgcgcggc ctgtagcgcg
    28501 atattacgta gttcaatctg ttgcggcatt agtgtgcgtg ctccgtcacc agatgaggat
    28561 ccagatgatc gcgcagtgcg tcacccacca ggttaaaggc catcacgctg ataaacagcg
    28621 ccagccccgg ccagaacatt tgcagcggct gggtccagat atactggcgc gcgtcgttaa
    28681 tcatcacgcc ccattcggcg gtcggcgcgg tcacaccgag gccaaggaaa gacatccccg
    28741 cgacgtgcag catcatatgg ccgatatcca gcgttgccag caccagcagc gaagggatca
    28801 ccgcgcctgc cagatgatcg acaaacaccc gcacatggcc cgcaccggaa agccgtgacg
    28861 ccagcacaaa ctcgcgttgg cgtagtgaaa tcaccaagct gcgcaccatg cgtgcatacc
    28921 acgcccagtg cgacagggcg atggcgataa ttacgttggt cagcccggtg ccgagcacgc
    28981 caaccataaa gaacgacaga atcgaggtcg ggaaggtcat aaacatatcg gcgacgcgca
    29041 tggtggcctg atcaacgcgc ccgccaatca acccggcgct gccgccaata accagcccta
    29101 atgtcagcac cagcagcagg caggccatta ccgaaccgag cgacacgcgg gtcgctgcca
    29161 tcagccgcga gaaaatatcg cgacctaagt gatcggtgcc cagccagtgc tgcgcatccg
    29221 gcgaaagcag gcgcgacggc aaatcaatcg cctgggggtc atacggcagc caccactggc
    29281 tggtgagcgc aatcagcgcc agcagggcga taatgatcag cgccaggcgt accgaccagc
    29341 gggaagagag gaaaaagttc acgcgtgcgc tccttcatga cgacgaatgc gcgggtccag
    29401 cgcggcgttg agcaaatcga caatcaaatt acagaccaca aaaaccacca ccatcatcag
    29461 cgtaaagcac tgaatcaccg gatagtcacg gttaaaaatc gccgacaccg catagcgccc
    29521 gacgcccggc caggcaaaga tgttttcgat aatcatcgtc ccgccaatca gttcgccgat
    29581 gtgcatcccc acggcggtga tcatcggcag cgaggcattg cgcagaatat ggcggcgttc
    29641 ggtctgtttg tcgttcaggc cgcgcagacg cgcccaagtg acgtgacgct gaccggcgac
    29701 gtccagcata ctggcgcgca gcagtcgcgc gttaatcgcc agcgacataa aggcaatgga
    29761 aaccgcaggc aaaatgatgt gctgccagcc gccgtaaccc atcgcgggta gccattgcag
    29821 atacaccgaa aacgccatca ccagtaaaaa cgccagccag aagttaggca tcgacacgcc
    29881 aagaaacgcg atgaaacgta cggcgaaatc cggcagacgg tcgcgatggc gcgccgccca
    29941 gataccgagc ggtacggaag tgagcagaat taataccagc gccgcacctg caagttccag
    30001 cgtggcgggc aggaagttca gcatatcgtc cagcaccggg cgttggctgg cgaatgagat
    30061 accaaagtca agatgcagcg ccttccacaa ccaggtaccg tactggacgt acagcggctg
    30121 atccagcccg agcatggtgc gggtagaggc cagcatctcc ggcgtcggcg gcaggttaga
    30181 taaacgcaga taatcgagcg ccgggtcgcc ggtaccgagg cgcagcatca gaaaaatgat
    30241 caccgaggcg gcgagcacca tcgggatcag cagcagaaag cggcgtaata cgtaacgcaa
    30301 cattaaggtt tcaccggttt aatctgttcg aacggaattt cggtggcgat cggcgcgtag
    30361 gggatattac ccagctccgg ttttgatacc accatcatcg agatgtagct gataggcaga
    30421 taaaccgcct cgtcatgcag acgggtcaaa atgtcgcgat acagcgcctg acgttgcgtt
    30481 tcgtcatggg tcgccagcac ttcgccgatc tctttatcaa tcagcggctt gtcggctaat
    30541 ccttgctgtg cctggaagtc agcgtgcgac ggtacgcgca ttgaactcag gaaggcgtgt
    30601 ggatcgtatg gcgcgcccca ggtgcggtgg aaaatcatgc caaaacgacc gtcgcgctga
    30661 cgagcataga tactgctctc ttcttcgcca atcagcgaga catctgcgcc aatctggcgc
    30721 atatcagcct gaatgatttc cgccatcgat ttgcttaacg catcggtgcc gatgaacgac
    30781 agttcaatgc gcagcggctg accatttttc tcgcggatgt ctttgcccgc aggcagcgtc
    30841 caaccggctt tttccagcaa ctctttcgct ttctgcggat cgtactggcg cggtttcagg
    30901 ccgagattag cgtaaggcac agaaggggca aacagggtgt cggcgacctg cggggtgcca
    30961 tataacgcgt tatcaatcag cgattttttg tttaccgcgt aattaagagc ttcacgtact
    31021 gccagctcgt tggtgggggc tttggcggta ttgagcgcca gcatcacggt ttcgatcggc
    31081 tgtgacagtt gggtgtggta agccggattc tggctaaagc gggcgaaggt atcgagcggt
    31141 aataaccctt cgtttccgta cagcaggtcg atatcgccag tttcaaacgc caccgcgcgg
    31201 gtagtcgggt ccgggatgac gttaaaggtg atctttttaa tcgctggctt ttcgccccag
    31261 tagttttcgt tacggacgaa gacatcgtac tgattcagtt tcgattcctg caaaatccac
    31321 ggtccggtgc caatcggcgc tttaattccg ttcatggttt catggttttt aaactgcgag
    31381 ggagcgataa aacggaaagg acggggcagg gccagttctt gcaggaaagg atagtaggcg
    31441 cttttcaggg taatttgcag ctctgtttta ctgagtgctt taacatcaac aatctggttt
    31501 gccagctcca gccaggcgtg acgttgacgg ttatcgagca ctgcgcggaa gttttctgcc
    31561 gccgcctcgg catcgaacgg ttcaccgttg gagaatttca cgtcatcacg cagggtgaag
    31621 gtccaggttt taccatcttc tgaatgagtc cagctttttg ccagccacgg gatcaccgaa
    31681 ccgtctgcct gatatttcac caatggttca taaaccatgc tctgggcgaa catctggtta
    31741 ggcgtgtaaa ggtgcgggtt tagtggcccg acattcaccg gccaggcggt agtgatttca
    31801 tctggtgcag cggcatggac gataaaagac gcacaagcca gcagcgcaaa tagagtgcgg
    31861 cggagtgtgg agagcatggt aaccccaatg gattaaaata gatggcggaa ataagtatga
    31921 cgattttaag tattcgtcat actgattacc tgttctggat caatagatgg gcagtcgggt
    31981 gggcggatca gttaactgaa tcgatccatt gcacactgtc ggcggtgagc gtaaaggggg
    32041 tgggggtgca gatcgcaagg cttaaatttt ctagctgaca atggctgact gacagcgagg
    32101 agtgataggt gctatctacg ctgacgattt gccaggcgct gccgccacgc tgtttaacga
    32161 tggcttcttt gcgcgtccag atgcgccaga acatttccag ttgctgatca ggatgcacgg
    32221 cgtccatttc agcgtgttcc ccgaggctga acacggcgtt cgccagccag cgccagttgg
    32281 cgcgcgggcg aatcacttcg atatcgcagc cgacttcgcc ttcatcgctc aacagcagag
    32341 cgatatcgtc accgctatgg cttaagttga accatagcgg cgtttccggc gcgaatgcag
    32401 gtttgccttg ttcgccatag atgatctccg gtagcgggga aagcgtgtgc gaaagcaatg
    32461 cacgccccgc cagccagcgt tcgcgtcgtg gaccttgcgg tgcttgctcg cgtaaacccg
    32521 gtggcagtgg agctgcgctt aaggtcgaaa ctttccccag aactatccga tacatatcag
    32581 ggccaacgtt taatggaaaa tgaaagtgcg tatcgtatca cttgtcgcct catcccggta
    32641 accgactttt cggtctgcct ggccccagta aaatcgccag tttgctaccg cctttggtgg
    32701 tttccatcca gattttacac acactggtta atggcaccga aagtagcatc cctaccgggc
    32761 cgagcagcca tccccaaatt aacaatgaaa gaaataccac catggtggac atccccaggc
    32821 gatggcccat catccgtggt tctaaaatat tgccgatgac catatggacc actaaaaaca
    32881 atgcgccgac cagaatacat tcgtaaacac cattaaacag cagcacctga atcattggcg
    32941 gtacggcgga aattaccgcg ccgatattgg gcacgtagtt gagcaaaaac gccagtactg
    33001 cccacatcag cgcaaactgc acccccatca gctccagccc cagccagacg atgacacctg
    33061 tccataaact gagtagcgtc ttcaatgcaa gatagtgcga aacgccttta agtgcgcggt
    33121 gtaatcctgc gatgtgaatc tgtgggttat tcagcgcaaa acgcattttg taagggacgt
    33181 ggcgcacttc aaacagcata aaaactacgg tcatcaccag caaaagcacg ctcgccattg
    33241 ccccggaaag cccggtcatt agcgctgtgg tgaaggtaac cactttttcc gagtccatcc
    33301 gctgcagcat tcgctccggc gacatatgca aattaagaaa aggcaacatc tcctgcaatt
    33361 taaaaagttt gcgcgtcagc tccttattaa acttcggcag catagagata aattcgttaa
    33421 acgatgccgc cagtacgccg accagcgcgg ttagtgcgat cagcatcacc accactacaa
    33481 tcgtaatggc aacggggcgt tgtactcccc gacgaataaa ccaggtaacg agcgggttga
    33541 ggacgatggc aaaaaacagc gccagtaaca gctgcacaat gatatctgcc gctgcgtgaa
    33601 tgcccgcgag gatcactacc agcgaggcca gcttgagcag aatgtgcatg cccgttttat
    33661 cgggttgagg gatttccatt ggggcttcct tgtgactttt tgtattaagt gtagcgggag
    33721 tcacgccagc aatattctga ttcttactgc tgattttcac gtcagcacgt ggtaaaaatg
    33781 aaacactgtt gtaaaaatgt ggtgatcctc atgcccgaac ccgtagccga acccgcgcta
    33841 aacggattgc gcctgaattt gcgcattgtc tccattgtca tgtttaactt cgccagctac
    33901 ctcaccatcg ggttgccgct cgctgtatta ccgggctatg tccatgatgt gatgggcttt
    33961 agcgctttct gggcaggatt ggttatcagc ctgcaatatt tcgccacctt gctgagccgt
    34021 cctcatgccg gacgttacgc cgatttgctg ggccccaaaa agattgtcgt cttcggttta
    34081 tgcggctgct ttttgagcgg tctgggatat ctgacggcag gattaaccgc cagtctgccc
    34141 gtcatcagcc tgttattact ttgcctggga cgcgtgatcc ttgggattgg gcaaagtttt
    34201 gccggaacgg gatcgaccct gtggggcgtt ggcgtggttg gctcgctgca tatcgggcga
    34261 gtgatttcgt ggaacggcat tgtcaattac ggggcgatgg cgatgggtgc gccgttaggc
    34321 gtcgtgtttt atcactgggg cggcttgcag gcgttagcgt taatcattat gggcgtggcg
    34381 ctggtggcca ttttgttggc gatcccgcgt ccgacggtaa aagccagtaa aggcaaacca
    34441 ctgccgtttc gcgcggtgct tgggcgcgtc tggctgtacg gtatggcact ggcactggct
    34501 tctgccggat ttggcgtcat cgccaccttt atcacgctgt tttatgacgc taaaggttgg
    34561 gacggtgcgg ctttcgcgct gacgctgttt agctgtgcgt ttgtcggtac gcgtttgtta
    34621 ttccctaacg gcattaaccg tatcggcggc ttaaacgtgg cgatgatttg ctttagcgtt
    34681 gagataatcg gcctgctact ggttggcgtg gcgactatgc cgtggatggc gaaagtcggc
    34741 gtcttactgg cgggggcagg gttttcgctg gtgttcccgg cattgggcgt agtggcggta
    34801 aaagcggttc cgcagcaaaa tcagggggcg gcgctggcaa cttacaccgt atttatggat
    34861 ttatcgcttg gcgtgaccgg accactggct gggctggtga tgagctgggc gggcgtaccg
    34921 gtgatttatc tggcggcggc gggactggtc gcaatcgcgt tattactgac gtggcgatta
    34981 aaaaaacggc ctccggaaca cgtccctgag gccgcctcat catcttaaaa cttactgaat
    35041 aaccagcgta ttaatgatgt tttctgcggt ggtctgcgct ttttgctgat cgtcagctgg
    35101 cagagtaatt tgcatggtca gcagttgatt acccacgtta cccagaataa cggaagagta
    35161 cgccgtctgg cctttcgcgg agataatact gtctaactgc tgcattttgt gacctttcag
    35221 ctcaatggct ttgttggtta ccacttgcag ctgcggatcg cggctacgtt gctgatcttc
    35281 cagacgcttc gccaacaccg ccagatcttc tttcggatca tcgcccatga tgacgatgac
    35341 tgctttctgc ccggtggcgt cggaccagac atgcatgtta ttggcctgcg ttcccagctt
    35401 accgctctgg tcggtcatat ccgctggcag cgagaaactt aacttgccat caagcaggtt
    35461 gacgggattc ccggtagcgt tactttccgc gaccgaaccc tgcgccgtag cgttagtgtc
    35521 tttatcatca caggccgcaa gccccataac cagcaggcca attccgacat atttaaccag
    35581 attgcgcatt gacttcttcc tttcgataaa cggccataac ggctcattca tccatcttat
    35641 cacaactctg ataacgaacc tttaactcgc ctgcaaagcg ttgatttcgg atttatctgc
    35701 cagtcttttc aacagaatat tgagtaatac gccatacatt ggcaggaaga aaacgatact
    35761 gattaacact ttgaaacagt aatcgaccag cgcgatttcc atccagtgtt cagccataaa
    35821 ggcatccggg ctacgccaga aggcaatgaa gaaaaaggcc agcgtgtcgc tgacgttacc
    35881 gaacagtgtg gacgctgtcg gtgccagcca ccagcggcga ctctgacgca ggcggttaaa
    35941 aacgtgcacg tcgaggatct gtcccagcgc gtaggccatg aaactggcgg tggcgatacg
    36001 ggcgacaaac aggttgaagt gggcgagtgc gccgaatccc tgccaggaac ccatatagaa
    36061 tagcgacgag atgacgtagg agatcagcaa cgccgggatc atcaccgcaa agatgatacg
    36121 acgggccagc ggtgcgccaa aaatacgcac ggtcaggtcg gtagcaagga aaataaacgg
    36181 gaagctaaac gcgccccagg tggtatggaa acccaaaatg gagacgggaa gctgaaccag
    36241 atagttactg gaggtgatca ccagcagatg aaataacgat aaccagaaca acgccttata
    36301 gcgttgagtt tgcgagaaaa cgttcatatt gtaccttttt gattaaccat tggggtgagg
    36361 gaacccaata cgtacgacac gtctgttacc ttaacataat aacgtgctta cccttttttc
    36421 gagccgccgc atgatactgc tttacgttga caatgcaatg gttaattttc acgcaatcgt
    36481 taacctggtt tgcttacgga ctcacagggc gtaaactagc gccgtttttt tatgtgatga
    36541 gaagaaaatg accgatctct tttccagccc tgaccacaca ctcgacgcac ttggcctgcg
    36601 ctgcccggaa cccgtaatga tggtgcgcaa aactgtgcgc aatatgcagc ctggcgaaac
    36661 gttgctgatt atcgccgacg atccggccac tacccgcgat attcctgggt tttgtacctt
    36721 tatggaacac gaactggttg cgaaagagac ggatggactg ccttatcgtt atttgattcg
    36781 taaaggcggt tgatggggaa agattggctt cgatgccgcc ttttcccctc accctaaccc
    36841 tctccccaga ggggcgaggg gaccgatcgc gctcaatgtt gcgatcggtt tgccttatct
    36901 cctgcgcaac aatcttaacg cattcgctgt caccagcacc gtcgcacccg tatctgccag
    36961 caccgccagc cacaggccgg tcatccctaa cagcgtggtg acgaggaaga tccctttcag
    37021 ccccagcgca atggtgatgt tctggcggat attggcgtga gtggcgcgtg ccagttcaat
    37081 catttgcacc aggccgcgca ggtggttatg ggttaatgct gcatcagcgg tttccagcgc
    37141 cacgtctgtg ccactgccca ttgcaatccc aatggcggca gctttcatcg ctggcgcgtc
    37201 gttaatacca tcaccgacca tcgccagggg cgcatgttga ttcagcttgg tcaccgcttt
    37261 gactttatct tccggcaata ggcccgcttt aaactccagc cccagctccc cggcgattgc
    37321 cgccgctgca cgtggattat cgccggtgag gatcactcct ttgaccccca gcgcgttcag
    37381 ttcactgatg gcagttgcag catcggcgcg cagggtatcc tgcaatgcaa tgatacccag
    37441 cacgtcatca ttacgtacta ccagcacaac cgtttgcccg gcgctttcca gttcattaat
    37501 caaaccagca aatgcatcag cgggatgttt cccggcagcg caaatcaata cgcgctcacc
    37561 gttaacctgc gcttcaatgc cagacccgac cagcgcccgc tgtgattcgg cggtgggaat
    37621 ggcgagttca gcaacctgtg cttcgcgtac gatggcttgc gccagtggat gcgtcgcgcc
    37681 ttgctcgacc gccgccgcca gtgtcagcag ttcagattca ctaatacccg ttgccggatg
    37741 aatcgcggta acgcgcggtt taccgacggt cagcgtaccg gttttatcaa acgccacctg
    37801 agtaacacga cctagttgtt ccagcgccgc tccgccttta atcaacgccc cacgacgcgc
    37861 tgccgccgcc agcccggagg taatcgccgc aggcgttgag ataactaacg cacacgggca
    37921 gccaatcagc agcagcgtca gccctttata aatccactcc tgccagctgg cggcaaacag
    37981 cagcggtggc accagcgtca ccagcagagc gacggccata atcgcgggcg tatagatacg
    38041 gctgaaacgg tcgataaacc gctcaatggg ggcgcgacgc tcttcggctt cttcgatcag
    38101 tttcagaatc cggtcaatgg cgctggcacc cggttctgac agcacttcca gcgttaccag
    38161 acggtctacg ctggtggctc ctgcaggaac tttatcgccc gtcgcgcgct ccaccggaat
    38221 ggattctccg gtcagggcgc tttcatcaaa actggcaaac ggtgagagca gtttaccgtc
    38281 ggcaggcaaa cgcccacctg cggcgacttc aatcacatcg ccaggccgca ggctgttaat
    38341 cgccacctct tcccgctcac cgttacgcag gcgcgtggcg gtttctggtt tcagcgccat
    38401 taacgcgcta acgccctgac gcgcgcggct ggcggcccag ccttccagtc gttcaccaat
    38461 caaaaacagc agcaacacca tcgcagcttc agccgttgcg ccaataaaca gtgcaccaat
    38521 agcggctacg ctcattaagg tttcaatggc gaagtagctg ccggatttga tcagccgtaa
    38581 tgcctgacga gcaatcgggt acagcccaac cagcgtggtc gcgataaacg ccagttgccc
    38641 gaacggatga ttgaactgct ccagacccca gctgattgcc atcatcacga ttagtgtaat
    38701 cagcggcaga ttctctttca ggcgcgatgc ttgtggctcg tcggcggcct gctcatcgcg
    38761 cagggaatag cccgcttttt gcaccgcaga ttcaacttgt gcacgaatgt cattgtcggc
    38821 atcaaccacc agtttttcgg tggcgaacaa cacctgcacc tgattcacac ctgcaagctg
    38881 gcgcacggca ttttctacct tgcgcgcaca ggcggcgcag tccatgccac tgactttcca
    38941 gctatagcgg gtgccggaga cgttttcaga gagcgttggc gagctggagc atgcgccgtc
    39001 gcagcaacag tcgttggcgt tctgtaccgt ggttagcggt ttgaacgcag caaattgagg
    39061 ggctttcttg ccgtgattgt caggtgtcga catggcatac tccggttaag ttttttctca
    39121 ttaaccgaag gatacactct ggagtcgact ccagagtcaa gttttatcag agatacagtg
    39181 agcggatgat gaggaagtga ccggcaaagt agcaggccgc agcaatcgcg ttatccgcac
    39241 ggaagcgtcg gcgatagtgg ctccccagcc agacaaagtt actgataaac agcaacgaag
    39301 cgccaacaaa cgcagagagc gccggagcgg tcggacggaa gaaccacagt tcacctgcca
    39361 gccacaccat caccagcgtc atgccgataa acgtgcagat aggccaacga tactcttcca
    39421 gccgcgtcca gataatcgcc agtaacagcg cacccagaac cagcaacacc agtggcagag
    39481 gccagaagaa agagagcgtc atctgactgg cgaaatagat ggtgtacagc aggtgcgaga
    39541 ggaaaaacgc gccgatggcg tacatcagac gttgacgtgg caacagggtt agcgcatcgc
    39601 ccagcagtga ggcgcacagc cctgccagca ccagatagct aatggcgtcg aacatcggcg
    39661 cttgccaggc cagtaacagc aggagaagaa gggttaacgg tttaaacacc cagcgttgcc
    39721 aggttggccc acgatacgat gcatccacag atagccatgc ggaaagacag acagcgataa
    39781 acgaccaaag catcttagtt ccttgaattc gttatttttt cgtaagcgga atgcctacgg
    39841 tcttgcttcc agtttagtgg cgagggtgga cggatgacaa caccataact ggcagggtat
    39901 gcttatttcc gcattttccg atgagggata aagatgagca aaccacctct tttctttatt
    39961 gttatcattg gcttaattgt cgtcgccgca tcgtttcgtt ttatgcaaca gcgacgggaa
    40021 aaagctgata atgatatggc tccgctccag caaaagctgg tggtggtgag caacaagcgg
    40081 gaaaaaccga ttaacgatcg ccgttcgcgc cagcaggaag tgactccggc aggcaccagt
    40141 atgcgctacg aggcaagctt caaaccgcaa agcggaggaa tggagcagac gtttcgcctc
    40201 gacgcccagc agtaccacgc cctgacagtg ggcgataaag gtacgctgag ctataaagga
    40261 acgcgctttg ttagctttgt tggcgaacaa taaggttatt ttttaacttt aaatttcttc
    40321 tgccagacca gcagttcaaa cacgccgaaa aggaaaatcc gtaccttttc ggcggtggtc
    40381 atctgcgggc catctttcgg taaagtggat ttcaatagcg ccagctgcat accatgcatc
    40441 agcacggtaa aaatcagcgc cacgttaacg aaaatattta gcgggcgcgg gaagggatgc
    40501 accagattga ggattaaaaa tccccaaacg cagagcatta acaaacgacc aatattaatc
    40561 agcatcactt tctccttgtg cttcgcgttg atacagccga taagccacct gacccgccac
    40621 tttttcccga tgtaacgacc agtttgccgg aacggtgggc agaccgtttt cgacttcgct
    40681 ttcgacataa atcagggctt cgtcagccag ccagccgtta tcttccagta aatttatcgt
    40741 ctcttctaac aagccacggc ggaacggtgg atcgacaaac acgatattat gcggtgtgcc
    40801 tttttgcgcc aggaatgaca tcgcgttgct gttcaccacg cgtgcattgc ctgcttttag
    40861 tgtcgccaga ttcttaatta actgctgaga aactgcacga tccatctcaa ttaacgtggc
    40921 ccccgcagcg tagcgcgata acgcttccag ccccagcgcg ccgctcccgg cgaagcaatc
    40981 cagacattgg gcgtcaacaa tgaccggagc cagccagtta aacaacgttt cgcgtacgcg
    41041 gtcggtggtg gggcgcagac ctgggctatc aggaaccggg agtttacggc ctcgccactg
    41101 cccgccaata atgcggattt ggccgctgcc ggaatgattc ggttttttca tgataaattg
    41161 ctcaatccgc cagataacac atacttgcag gcggtgatgt gaattaagtt aagtgataga
    41221 ctatttcatc atttttttag ctgctatgta catagcgtta acgctgtgcc atgaagcaac
    41281 agcgaggagt gtagtcgcaa atggcgaaag aaaaaaaacg tggctttttt tcctggctgg
    41341 gctttggtca aaaagagcag accccggaaa aagagacaga agttcagaat gaacaaccgg
    41401 ttgtagaaga aatcgttcag gcgcaagagc ctgtgaaggc ttcggaacaa gccgttgaag
    41461 agcagccgca ggcgcatact gaagccgagg cggaaacttt tgctgccgac gttgtggaag
    41521 tcactgaaca ggttgttgaa agtgaaaaag cgcagcctga agcggaagtt gttgcacagc
    41581 cggaaccggt cgtagaagaa acgccggagc cagtggctat cgaacgtgaa gagctgccgt
    41641 tgccggaaga cgtcaacgcc gaagaggttt cgccagaaga gtggcaggct gaagcggaaa
    41701 ccgtagagat tgtcaaagcg gcggaagaag aagcggctaa agaagaaatc accgacgaag
    41761 agctggaagc acaggcgctg gcagccgaag cagcagaaga ggcggtgatg gtggttcctc
    41821 cggcagaaga agagcagccg gtggaagaaa tcgctcagga gcaggaaaaa ccaaccaaag
    41881 aaggtttctt cgcgcgcctg aaacgcagcc tgttaaaaac caaagaaaat ctcggttccg
    41941 gatttatcag cctgttccgc ggtaaaaaaa tcgacgatga tctgtttgag gagctggaag
    42001 agcagctttt gatcgccgat gtgggcgtgg aaaccacacg taaaattatc accaatctga
    42061 cggaaggcgc atcccgcaag cagcttcgtg atgccgaggc gctctatggc ctgctgaaag
    42121 aagagatggg tgagattctg gcgaaagtcg atgagccgct gaatgttgaa ggtaaaacgc
    42181 cgttcgtgat cctgatggtg ggcgtcaacg gtgtgggtaa aaccacgacg attggtaagc
    42241 tggcacgtca gtttgagcag cagggtaaat cggtgatgct ggcggcgggc gatactttcc
    42301 gtgcagcagc ggttgaacag cttcaggtct ggggtcagcg caacaatatt ccggtgattg
    42361 cccagcatac cggggcggat tccgcctctg ttatcttcga cgccattcag gcagctaaag
    42421 cgcgtaatat cgacgtcctg attgccgata ccgccggacg cctgcagaac aaatcgcacc
    42481 tgatggaaga gttgaagaaa atcgtccgcg tgatgaagaa actcgacgtt gaagcgccgc
    42541 atgaagttat gctgactatt gatgccagca ccgggcagaa cgcggtaagc caggccaaac
    42601 tgttccacga agctgttggc ttaaccggca tcacgctaac gaaactggac ggcacggcga
    42661 aaggcggggt aattttctcg gtggctgacc agtttggtat ccctatccgc tacattggtg
    42721 tcggcgaacg tattgaggat ttgcgtccgt ttaaggcgga cgactttata gaggcacttt
    42781 ttgcccgaga ggattaacaa tgattcgctt tgaacatgtc agcaaggctt atctcggtgg
    42841 gagacaggcg ctgcagggcg ttacgttcca tatgcagccg ggtgagatgg cgtttctgac
    42901 cggtcattcc ggcgcaggga aaagtaccct cctgaagctg atctgtggga ttgagcggcc
    42961 cagcgccggg aaaatctggt ttagcggcca tgacatcacg cgtctgaaaa accgtgaagt
    43021 tccgtttctg cgccgccaga ttggcatgat tttccaggat caccatctac tgatggaccg
    43081 tactgtctac gataacgtgg cgatcccgct gattatcgct ggtgccagcg gtgacgatat
    43141 tcgtcgccgg gtgtcggcgg cactggataa agtcgggcta ctggacaaag cgaagaactt
    43201 ccctattcag ctttccggcg gtgaacaaca gcgtgttggc attgcccgcg cggtggtgaa
    43261 taagcccgcg gtactgctgg cggacgaacc gaccggtaac ctggacgacg cgttgtcgga
    43321 agggattcta cgtctgtttg aagagtttaa ccgcgttggg gtaaccgtat tgatggcaac
    43381 gcacgacatc aacctgattt cacggcgttc ccatcgcatg ctcaccctga gcgatggtca
    43441 cttgcatgga ggcgtgggcc atgaataagc gcgatgcaat caatcatatt cggcagtttg
    43501 gtgggcgtct ggatcgcttc cgtaaatcgg tcggcggctc aggcgacggc ggtcgtaacg
    43561 caccgaaacg cgcgaaatcc tcgccaaaac cggtaaatcg caaaaccaac gttttcaacg
    43621 aacaggtgcg ctatgccttc cacggcgcat tgcaggatct gaaaagcaaa ccgttcgcca
    43681 cgtttttaac ggtgatggtt atcgccatct ccctgacgct gcccagcgtc tgttatatgg
    43741 tgtacaaaaa cgttaatcag gcggcgacgc agtattatcc gtcaccgcaa atcactgttt
    43801 acttgcaaaa aacgctggac gatgacgccg ctgcgggcgt ggtggcacag ttgcaggccg
    43861 agcaaggcgt ggagaaagtg aactatcttt ctcgtgaaga cgcactgggc gagttccgta
    43921 actggtctgg ttttggtggt gcgctggata tgctggaaga aaacccgctt ccggcagtgg
    43981 cggtggtgat cccgaaactc gattttcagg ggacggaatc actcaatacg ctgcgtgatc
    44041 gtatcacgca gattaacggc attgacgaag tgcggatgga tgacagctgg tttgcccgtc
    44101 tggcggcgtt gaccgggctg gtcgggcgcg tttcggcgat gatcggcgtg ttgatggtgg
    44161 cggcagtgtt cctcgtcatc ggcaacagcg tacgtctgag catctttgct cgccgtgact
    44221 ccatcaacgt gcagaaactg attggtgcga cggatggttt catccttcgc ccgttcctgt
    44281 atggtggcgc actgctggga ttttctggcg cattgttgtc attaattttg tcagaaattc
    44341 tggtgctgcg attgtcatcg gcggttgcgg aagtggcaca ggttttcgga acgaagtttg
    44401 atatcaatga tttatcgttc gatgaatgcc tgctattgct gctggtatgc tcgatgattg
    44461 gctgggtggc agcgtggctt gccacggtac aacatttacg ccactttacg cctgaataat
    44521 aaaagcgtgt tatactcttt ccctgcaatg ggttccgtag cagggaaaga gatcccgttg
    44581 tctcttcccg gtatttcatc tctatgtcac attttgtgcg taatttattc acaagcttgc
    44641 attgaacttg tggataaaat cactgtctga taaaacagtg gatgataacc tcgttgctct
    44701 taagctctgg cacagttgtt gctaccactg aagcgccaga agatatcgat tgagaggatt
    44761 tgaatgactg acaaaatgca aagtttagct ttagccccag ttggcaacct ggattcctac
    44821 atccgggcag ctaacgcgtg gccgatgttg tcggctgacg aggagcgggc gctggctgaa
    44881 aagctgcatt accatggcga tctggaagca gctaaaacgc tgatcctgtc tcacctgcgg
    44941 tttgttgttc atattgctcg taattatgcg ggctatggcc tgccacaggc ggatttgatt
    45001 caggaaggta acatcggcct gatgaaagca gtgcgccgtt tcaacccgga agtgggtgtg
    45061 cgcctggtct ccttcgccgt tcactggatc aaagcagaga tccacgaata cgttctgcgt
    45121 aactggcgta tcgtcaaagt tgcgaccacc aaagcgcagc gcaaactgtt cttcaacctg
    45181 cgtaaaacca agcagcgtct gggctggttt aaccaggatg aagtcgaaat ggtggcccgt
    45241 gaactgggcg taaccagcaa agacgttcgt gagatggaat cacgtatggc ggcacaggac
    45301 atgacctttg acctgtcttc cgacgacgat tccgacagcc agccgatggc accggtgctc
    45361 tatctgcagg ataaatcatc taactttgcc gacggcatcg aagatgataa ctgggaagag
    45421 caggcggcaa accgtctgac cgacgcgatg caaggtctgg acgaacgcag ccaggacatc
    45481 atccgcgcgc gctggctgga cgaagacaac aagtccacgt tgcaggaact ggctgaccgt
    45541 tacggtgttt ccgctgaacg tgtacgccag ctggaaaaga acgcgatgaa aaaactgcgc
    45601 gctgctatag aagcgtaatt tccgctatta agcagagaac cctggatgcg agtccggggt
    45661 ttttgttttt tgggcctcta caataatcaa tttcccctcc ggcaaaacgc caatccccac
    45721 gcagattgtt aataaactgt caaaatagct attccaatat cataaaaatc gggtatgttt
    45781 tagcagagta tgctgctaaa gcacgggtag ccatgcataa aacgaaataa agtgctgaac
    45841 aacaacatca caacacacgt aataaccaga agaatgggga ttctcaggat gaacataaag
    45901 ggtaaagcgt tactggcagg atgtatcgcg ctggcattca gcaatatggc tctggcagaa
    45961 gatattaaag tcgcggtcgt gggcgcaatg tccggtccgg ttgcgcagta cggtgaccaa
    46021 gagtttaccg gcgcagagca ggcggttgcg gatatcaacg ctaaaggcgg cattaaaggc
    46081 aacaaactgc aaatcgtaaa atatgacgat gcctgtgacc cgaaacaggc ggttgcggtg
    46141 gcgaacaaag tcgttaacga cggcattaaa tatgtgattg gtcacctctg ttcttcatca
    46201 acgcagcctg cgtctgacat ctacgaagac gaaggcattt taatgatcac cccagcggca
    46261 accgcgccgg agctgaccgc ccgtggctat cagctgatcc tgcgcaccac cggcctggac
    46321 tccgaccagg ggccgacggc ggcgaaatat attcttgaga aagtgaaacc gcagcgtatt
    46381 gctatcgttc acgacaaaca gcaatacggc gaaggtctgg cacgagcggt gcaggacggc
    46441 ctgaagaaag gcaatgctaa cgtggtgttc tttgatggta tcaccgccgg ggaaaaagat
    46501 ttctctacgc tggtggcgcg tctgaagaaa gagaatatcg acttcgttta ctacggcggt
    46561 taccacccgg aaatggggca aatcctgcgt caggcacgcg cggcagggct gaaaactcag
    46621 tttatggggc cggaaggtgt ggctaacgtt tcgctgtcta acattgcggg cgaatcagcg
    46681 gaagggctgc tggtgaccaa gccgaagaac tacgatcagg ttccggcaaa caaacccatt
    46741 gttgacgcga tcaaagcgaa aaaacaggac ccaagcggcg cattcgtttg gaccacctac
    46801 gccgcgctgc aatctttgca ggcgggcctg aatcagtctg acgatccggc tgaaatcgcc
    46861 aaatacctga aagcgaactc cgtggatacc gtaatgggac cgctgacgtg ggatgagaaa
    46921 ggcgatctga aaggctttga gttcggcgta tttgactggc acgccaacgg cacggcgaca
    46981 gatgcgaagt aatcattaat cggcaacttt gggttgccgc caaattgcta atatcgagta
    47041 cgttgcttca tgccggatgc ggcgtaaacg ccttatccgg cctataagat ccaaagaaat
    47101 cagtaaattg caacacacat tgtaggcctg ataagcgtag cgcatcaggc aatttctcgt
    47161 tggtcatcac ttttgaaatc aacttgacga ttaacgcttc tcccagccgc cctgttgcgc
    47221 cgtaaatccc agtgcctgca taaacgctgt catcacaccg cgatcttcca cgcctgcatc
    47281 cgccatccac cagcatgaaa cgccaggatt gttacgcaaa acctcttcca gcagatattg
    47341 ccccacaccg cgacgtcggg tgacttcccg cacacgcagg gaatccagtg ctccctcggt
    47401 gccgcttaag gttacccgta cggcagcgag caggcgctcg ttaaaacgcg cggcgtagat
    47461 acggtggtta tcgtcaacct gtaaagagga aggggaatac tccggccaga tcttttgcag
    47521 gtcaatccgg tcctggtcgc taaagttttc taatcgaatg atggtcagct tcatgggtaa
    47581 cccgtgtaaa tcacaaaagt ggaaccagtg tagcgaaata atttaatcgg aggctttctc
    47641 ttttttattt cttttggcag gtgattaatt ttttaacagc aataattaca aagttaaaac
    47701 attagagaat gaaaaatacc cagcataatc ccctgaatgg tagtgaatta ttccgccctt
    47761 tgtgccgtta ttttatgctg acaaaggcac ttttttctgt ttatctatca ataaattcag
    47821 aatattatct gttcttaatc gactgaaaaa tagggatttt aatcactgtt atcacaaaat
    47881 actgcgctaa acccttaatc agacaggcaa aaacagtgca gtataaaaaa agaacagtct
    47941 gatttgttaa cacataaaaa caaagcaaca caacatcacg aatggggatt tttgactatg
    48001 aaacggaatg cgaaaactat catcgcaggg atgattgcac tggcaatttc acacaccgct
    48061 atggctgacg atattaaagt cgccgttgtc ggcgcgatgt ccggcccgat tgcccagtgg
    48121 ggcgatatgg aatttaacgg cgcgcgtcag gcgattaaag acattaatgc caaaggggga
    48181 attaagggcg acaagctggt tggcgtggaa tatgacgacg cctgcgaccc gaaacaagcc
    48241 gttgcggtcg ccaacaaaat cgttaatgac ggcattaaat acgttattgg tcatctgtgt
    48301 tcttcttcta cccaacctgc atcagatatc tacgaagacg aaggtattct gatgatctcg
    48361 ccgggagcga ccaacccgga gctgacccaa cgcggttatc aacacattat gcgtactgcc
    48421 gggctggact cttcccaggg gccaacggcg gcaaaataca ttcttgagac ggtgaagccc
    48481 cagcgcatcg ccatcattca cgacaaacaa cagtatggcg aagggctggc gcgttcggtg
    48541 caggacgggc tgaaagcggc taacgccaac gtcgtcttct tcgacggtat taccgccggg
    48601 gagaaagatt tctccgcgct gatcgcccgc ctgaaaaaag aaaacatcga cttcgtttac
    48661 tacggcggtt actacccgga aatggggcag atgctgcgcc aggcccgttc cgttggcctg
    48721 aaaactcagt ttatggggcc ggaaggtgtg ggtaacgcat cattgtcgaa tattgctggc
    48781 gatgctgccg aaggcatgtt ggtcactatg ccaaaacgct atgaccagga tccggcaaac
    48841 cagggcatcg ttgatgcgct gaaagcagac aagaaagatc cgtccgggcc ttatgtctgg
    48901 atcacctacg cggcggtgca atctctggcg actgcccttg agcgtactgg cagcgatgag
    48961 ccgctggcgc tggtgaaaga tttaaaagct aacggtgcaa acaccgtgat tgggccgctg
    49021 aactgggatg aaaaaggcga tcttaaggga tttgattttg gtgtcttcca gtggcacgcc
    49081 gatggttcat ccacggcagc caagtgatca tcccaccgcc cgtgaaatgc gggcgggttt
    49141 agaaaggtta ccttatgtct gagcagtttt tatatttctt gcagcagatg tttaacggcg
    49201 tcacgctggg cagtacctac gcgctgatag ccatcggcta caccatggtt tacggcatta
    49261 tcggcatgat caacttcgcc cacggcgagg tttacatgat tggtagctat gtctccttta
    49321 tgatcatcgc cgcactgatg atgatgggca ttgataccgg ctggctgctg gtggcggcgg
    49381 gattcgtcgg cgcaatcgtc attgccagcg cctacggctg gagtatcgaa cgggtagcct
    49441 atcgcccggt gcgtaactct aagcgcctga ttgcgctcat ctcggcaatc ggcatgtcca
    49501 tcttcctgca aaactacgtt agcctgaccg aaggttcgcg tgacgtggcg ctgcccagtc
    49561 tgtttaacgg tcagtgggtg gtggggcata gcgaaaactt ctctgcctct attaccacca
    49621 tgcaggcagt gatctggatt gttactttcc tcgccatgct ggcgctgacg attttcattc
    49681 gttattcccg tatggggcgc gcctgccgtg cctgcgcgga agatctgaaa atggcgagcc
    49741 tgcttggcat caacaccgac cgggtgattg ctttgacctt tgttattggc gcggcgatgg
    49801 cggcagtggc tggcgtactg ctcggtcagt tctacggcgt cattaacccc tatatcggct
    49861 ttatggccgg gatgaaagcc tttaccgctg cggtgctcgg tgggattggc agcattccag
    49921 gggcgatgat tggcgggctg atcctgggga ttgcggaggc gctctcttct gcctatctga
    49981 gtacggaata taaagatgtg gtctcattcg ccctgctgat tctggtgctg ctggtgatgc
    50041 cgaccggtat tctgggtcgc ccggaggtag agaaagtatg aaaccgatgc atattgcaat
    50101 ggcactgctc tctgccgcga tgttctttgt cctcgctggc gtctttatgg gcgtgcaact
    50161 ggagctggat ggcaccaaac tggtggtcga cagcgcttcg gatgtccgtt ggcagtgggt
    50221 gtttatcggc acggcggtgg tctttttctt ccagcttttg cgacctgctt tccagaaagg
    50281 gctaaaaagc gtttccgggc cgaagtttat tctgcccgcg attgatggct ccacggtgaa
    50341 gcagaaactg ttcctcgtgg cgctgctggt gcttgcggtg tcgtggccgt ttatggtgtc
    50401 acgtgggacg gtggatatcg ccactctgac catgatctac attatcctcg gtctcgggct
    50461 gaatgtggtt gttggtcttt ctggtctgct ggtgctgggg tacggcggtt tttacgccat
    50521 cggcgcttac acttttgcgc tgctcaatca ctattacggt ctggggttct ggacctgttt
    50581 gccgattgcc ggactgatgg cagcggcggc gggcttcctg cttggttttc cggtgttgcg
    50641 tctgcgcggt gattatctgg cgatcgttac cctcggtttc ggcgaaattg tgcgcatatt
    50701 gctgctcaat aacaccgaaa ttaccggcgg cccgaacggt atcagtcaga tcccgaaacc
    50761 gactttcttc ggccttgagt tcagccgtac cgcccgtgaa ggcggttggg acacgttcag
    50821 taatttcttt ggcctgaaat acgatccctc cgatcgcgtc atcttcctgt atctggtggc
    50881 gttgctgctg gtggtgctaa gcctgtttgt cattaaccgc ctgctgcgga tgccgctggg
    50941 gcgtgcgtgg gaagcgttgc gtgaagatga gatcgcctgc cgttcgctgg gcttaagccc
    51001 gcgtcgtatc aagctgactg cctttaccat cagtgccgcg tttgccggtt ttgccggaac
    51061 gctgtttgct gcgcgccagg gctttgtcag cccggaatcc ttcacctttg ctgaatcggc
    51121 gtttgtactg gcgatagtgg tgctcggcgg tatgggatcg cagtttgcgg tgattctggc
    51181 ggcaattttg ctggtggtat cgcgcgaatt gatgcgtgat ttcaacgaat acagcatgtt
    51241 aatgctcggt ggtttgatgg tgctgatgat gatctggcgt ccgcagggct tgctgcccat
    51301 gacgcgcccg caactgaagc tgaaaaacgg cgcagcgaaa ggagagcagg catgagtcag
    51361 ccattattat ctgtgaacgg cctgatgatg cgcttcggcg gcctgctggc ggtgaacaac
    51421 gtcaatcttg aactgtaccc gcaggagatc gtctcgttaa tcggccctaa cggtgccgga
    51481 aaaaccacgg tttttaactg tctgaccgga ttctacaaac ccaccggcgg caccatttta
    51541 ctgcgcaatc agcacctgga aggtttaccg gggcagcaaa ttgcccgcat gggcgtggtg
    51601 cgcaccttcc agcatgtgcg tctgttccgt gaaatgacgg taattgaaaa cctgctggtg
    51661 gcgcagcatc aacaactgaa aaccgggctg ttctctggcc tgttgaaaac gccttctttc
    51721 cgtcgcgccc agagcgaagc gctcgaccgc gccgccacct ggcttgagcg cattggtttg
    51781 ctggaacacg ccaaccgtca ggcgagtaac ctggcctatg gtgaccaacg ccgtctggag
    51841 attgcccgct gcatggtgac gcagccggag attttaatgc tcgacgaacc ggcggcaggt
    51901 cttaacccaa aagaaaccaa agagctggat gagctgattg ccgaactgcg caatcatcac
    51961 aacaccacta tcttgttgat tgaacacgat atgaagctgg tgatggggat ttcagaccga
    52021 atttatgtgg tcaatcaggg gacgccactg gcaaacggta cgccggagca aatccgtaat
    52081 aacccggacg tgatccgtgc ctatttaggt gaggcataag atggaaaaag tcatgttgtc
    52141 ctttgacaaa gtcagcgccc actacggcaa aatccaggcg ctgcatgagg tgagcctgca
    52201 tatcaatcag ggcgagattg tcaccctcat tggcgcgaac ggggcgggga aaaccacctt
    52261 gctcggcacg ttatgcggcg atccgcgcgc taccagcggg cgaattgtgt ttgatgataa
    52321 agacattacc gactggcaga cggcgaaaat catgcgcgaa gcggtagcga ttgtcccgga
    52381 agggcgtcgc gtcttctcgc ggatgacggt ggaagagaac ctggcgatgg gcggcttttt
    52441 tgccgaacgc gaccagttcc aggagcgcat aaagtgggtg tatgaactgt tcccacgtct
    52501 gcatgagcgc cgtgttcagc gggcaggcac catgtccggc ggtgagcaac aaatgctggc
    52561 gattggtcgc gcgctgatga gcaacccgcg tttgctgcta cttgatgagc catcgcttgg
    52621 tcttgcgccg attatcatcc agcaaatttt cgacaccatc gagcagctgc gcgagcaggg
    52681 gatgactatc ttcctcgtcg agcagaacgc caaccaggcg ctaaagctgg cggatcgcgg
    52741 ctacgtgctg gaaaacggcc atgtggtgct ctccgatact ggtgatgcac tactggcgaa
    52801 tgaagcggtg agaagtgcgt atttaggcgg gtaataacac gttgattgat aaggagtcaa
    52861 aagactcctt tgagacaggt gacaaatgta aaattgcctg atgcgctgcg cttatcaggc
    52921 ctactgggtg agttgcaata tattgaattt gcacgatctt gtaggccgga taaggcgttt
    52981 acgccgcatc cggcatgaaa cgatgagtaa tctgtagagt ttgattcaga ccttctattt
    53041 ttccgcttat ccgtgcccca tctcccattt tccctcaccc acgccgtcac cgccttgtca
    53101 tctttctgac accttacttg caggtttatg aagttggcac cgccaccatg atggcatcga
    53161 aagccattaa accggtatat gacgtgttta aagaggcagg gattcaattc gatgagtcgc
    53221 agtttgtgcc gacggtttcc ggttactact ccgacagcaa aacgggccac ttactctccc
    53281 agccattcaa cagctcgact cccgttctct attacaacaa agacgccttc aagaaagcgg
    53341 ggttagaccc ggaacagccg ccgaaaacct ggcaggatct ggcggactct tccgcaaaac
    53401 tgaaagcctc cggtatgaag tgcggctacg ccagcggctg gcagggctgg atccaactgg
    53461 aaaactttag cgcctggaac ggtctgccgt ttgccagcaa aaacaacggc tttgacggca
    53521 cggacgcggt gctggagttc aacaagccgg agcaggtgaa acacattgcc atgctcgaag
    53581 agatgaacaa gaagggcgac ttcagctacg tcggtcgtaa ggatgaatcc accgagaagt
    53641 tctataacgg tgattgcgcg atgaccaccg cctcttccgg ttctctcgct aatattcgtg
    53701 agtacgccaa atttaactat ggcgtaggca tgatgcctta cgacgccgat gcgaaagatg
    53761 caccgcaaaa cgccattatc ggcggggcca gcctgtgggt gatgcagggc aaaaataaag
    53821 aaacgtatac cggcgtggcg aagttcctcg acttcctcgc gaagccagaa aacgctgccg
    53881 agtggcatca gaaaaccggt tatctgccaa tcaccaaagc agcgtatgac ctgacccgtg
    53941 agcagggctt ttatgagaaa aacccagggg cggataccgc gacgcgtcag atgctgaata
    54001 agccgccgtt gccgttcacc aaagggctgc gtctgggcaa catgccgcag atccgcgtga
    54061 ttgtggatga agagctggag agcgtgtgga ccggtaagaa gacaccacag caggcactgg
    54121 ataccgccgt tgagcgtgga aatcagttgc tgcgccgctt tgagaaatcg acgaagtctt
    54181 aatcagtgta atgtcggatg cgtttcgctt atctgacctg gcatcgcgtg taggccggat
    54241 aagcgaagcg catccggcac agttcaggaa ttaaccgtaa tgtcatcatc ccgtccggtg
    54301 ttccgctcgc gctggctgcc ttatctgctg gtcgcgccgc agctcatcat caccgttatc
    54361 ttttttatct ggcctgcggg cgaagcgttg tggtactcgc ggcaaagcgt cgatccgttt
    54421 ggtttctcca gccagtttgt cggtctggat aacttcgtca cgctgttcca tgacagctac
    54481 tatctagact ccttctggac gacgataaaa ttcagcacct ttgtcaccgt cagcggtttg
    54541 ctggtatcgc tgttctttgc ggcgctggtg gagtacatcg tgcgcggcag ccgtttctat
    54601 caaaccttga tgctgctgcc gtatgccgtg gctcccgccg ttgccgccgt attgtggatc
    54661 ttcctgttta accccggtcg cgggctgatc acccattttc tcgctgagtt cggctacgac
    54721 tggaaccacg cgcaaaacag cggtcaggca atgtttctgg tggtgtttgc ctcggtgtgg
    54781 aagcaaatca gctacaactt tctgttcttc tatgccgcgc tgcactccat tccccgttcg
    54841 ttgattgaag ccgcagctat tgacggcgca gggccgattc gccgcttctt taagattgct
    54901 ctgccgctta tcgctccggt gagtttcttc ctgctggtgg tgaacctggt gtacgccttc
    54961 ttcgacacct tcccggtgat cgacgccgcc acgtccggcg ggccggttca ggccaccact
    55021 acgctgattt ataagatcta ccgcgaaggc tttaccggac tggatctggc ttcgtctgcc
    55081 gcgcagtcgg tggtgttgat gttcctcgtc atcgtgctga cggtggtgca gttccgctat
    55141 gttgaaagca aggtgcgtta ccaatgattg agaaccgtcc gtggctgacg atattcagcc
    55201 ataccatgct gatcctcggg atcgcggtga tcctcttccc gctgtacgtg gcgtttgtcg
    55261 cggcgacgct ggataaacag gccgtctatg ccgcgccgat gacgctcatc cccggcacac
    55321 atctgctgga aaatatccac aacatctggg ttaacggggt aggcacgaat agcgcgccgt
    55381 tctggcggat gttgcttaac agctttgtga tggcgttcag cattacgctc ggcaaaatta
    55441 ccgtctcgat gctctcggca tttgccattg tctggtttcg ttttccgcta cgtaacctct
    55501 tcttctggat gatttttatc accctgatgc tgccggttga agtacgtatc ttcccgacgg
    55561 tggaagtcat cgccaacctg aaaatgctcg acagctacgc cggtttaacg ctgccgctga
    55621 tggcctcggc gaccgccacg tttctgttcc gccagttctt tatgacccta ccggatgaat
    55681 tggtggaagc cgcgcggatc gacggcgcgt caccaatgcg tttcttttgc gacatcgttt
    55741 ttccgttgtc caaaactaat ctggcggcgc tgtttgtgat cacctttatc tacggctgga
    55801 atcagtattt gtggccgttg ttgattatta ccgacgttga tctcggcacc accgtggcag
    55861 ggatcaaagg gatgatcgct acaggcgaag gcaccacgga atggaactcg gtaatggcgg
    55921 cgatgttgtt aacgcttatc cctccggtgg tgattgtttt agtgatgcag cgtgccttcg
    55981 tgcgcggcct ggtcgatagt gagaaataag atggcaggac tgaaattaca ggcagtaacc
    56041 aaaagctggg atggcaaaac ccaggtcatt aaaccgctga cccttgatgt ggcggatggc
    56101 gaatttatcg tgatggtcgg gccgtctggc tgcgggaaat cgacgctgct gcgcatggtt
    56161 gccgggctgg agcgggtgac agaaggcgat atctggatca acgaccagcg cgtgaccgaa
    56221 atggagccga aagatcgcgg gattgcgatg gtgttccaga attacgcgct ttatccgcat
    56281 atgagcgtag aggagaacat ggcgtggggg ctgaaaattc gcggcatggg caagcagcaa
    56341 attgccgagc gcgttaaaga agcggcgcgc attctggaac tggacggtct gcttaagcgt
    56401 cgtccgcgtg agctatccgg cggtcagcgc cagcgtgtgg cgatgggccg cgcgattgtg
    56461 cgcgatccgg cggtgttctt gtttgatgag ccgctctcta acctcgatgc caagctgcgc
    56521 gtgcagatgc gtcttgaact gcaacagttg caccgtcgcc tgaaaacgac ttcactctac
    56581 gttactcacg atcaggttga agcgagtaat ggtgatgaac ggtggcgttg ccgaacagat
    56641 tggcacacca gttgaagtct acgaaaagcc cgccagcctg tttgtggcga gttttatcgg
    56701 cagcccggcg atgaacctgc tgacaggccg cgtgaataat gaaggcacgc atttcgaact
    56761 ggacggcggt atcgtgctac cgctaaacgg tggctaccgt cagtatgccg ggcgtaaaat
    56821 gactctcggc attcgcccgg aacatatagc gctaagctcg caggcagaag gtggtgtgcc
    56881 gctggtgatg gacacgctgg agatcctcgg cgcagataac ctggcgcacg gacgctgggg
    56941 cgagcagaag ctggtggtgc gactggcgca tcaggagcgc ccgacggcag gcagcacgct
    57001 gtggctgcat ttggcggaaa atcagctgca tctttttgat ggtgaaacag gacaacgagt
    57061 atgagtaact ggccttatcc ccgcatcgtc gctcatcgtg gcggcggtaa gctggccccg
    57121 gaaaacaccc tggcggcaat cgacgtcggg gcaaaatacg gtcataagat gatcgaattt
    57181 gacgcgaagt tatcgaaaga tggcgagatc ttcctgctcc atgacgacaa tctcgaacgc
    57241 accagcaacg gctggggcgt cgcgggtgaa ctgaactggc aggatttact gcgcgtggat
    57301 gcgggcagtt ggtacagcaa agcgtttaaa ggtgagccgc tgccgttgct ttcgcaggtg
    57361 gcggaacgct gtcgcgaaca cgggatgatg gcgaatatcg aaatcaaacc caccactggc
    57421 actggaccat taacgggcaa aatggtggcg ctggcggcac gccaactgtg ggccggtatg
    57481 acgccgccgc tgctgtcatc gtttgagatt gatgctttag aagctgcaca acaggcggca
    57541 ccggaactgc cgcgtggttt attgcttgat gagtggcgcg acgactggcg cgaactgacc
    57601 gcgcggctgg gctgcgtctc tattcatctc aatcataagt tactcgataa agcgcgagtg
    57661 atgcagttga aagacgccgg attacggatt ctggtttata ccgtcaataa accccagcgc
    57721 gcggcagagt tgctgcgctg gggcgtggat tgcatctgta ccgatgcgat tgacgtgatt
    57781 ggtccgaact ttacggccca atagttttca acggaatgtc aggctgcggc gtgctgggct
    57841 gattcagcat gtcgccgttt ctcctttccg gcagcatatg ctgctgattc aacgaactat
    57901 ccggattacg gttgctgttt aacatcccgc cgttggtgtt gggcagcatt tgctgccggg
    57961 cgggatttcg ctcccccggc tgtgactgca acacccgctg agaattattg tttatctggt
    58021 tttctaaatg ctgctgttgc aactgagttt gcgttttcag ttgctgattc agcatccctt
    58081 tttgctggat ttgctgagtc tgcatctggg tttgcatccg ctgctggctg ggtatctgat
    58141 accccggctg gttagggttg ttcagagtat taatgggctg tgcaaagccg acaaacggca
    58201 ggagtgccgt aagaatcaga agtcgtttca tcgcgtatcc tcctctgaag atatccttta
    58261 agtttactcg cttcccgaca aaacgatgat taattcagag ttatatacca ggcttagctg
    58321 gggttgcccc ttaatctctg gagaataacg atgataaaac cgacgttttt acgccgggtg
    58381 gccattgctg ctctgctctc aggaagttgt tttagcgccg ccgccgcgcc tcctgcgccg
    58441 cccgtctcgt atggtgtgga ggaagatgtc ttccacccgg tacgcgcgaa acagggaatg
    58501 gtagcgtctg tggacgccac tgccactcag gtgggggtgg atatcctcaa ggagggcggg
    58561 aatgccgttg atgccgccgt ggcggtgggc tacgcgctgg cggtaacgca tccgcaggca
    58621 gggaatctgg gcggtggtgg ttttatgtta atccgctcga aaaatggcaa taccacggct
    58681 atcgatttcc gcgaaatggc acccgccaaa gcgacccgcg atatgttcct cgatgatcag
    58741 ggcaacccgg acagcaaaaa atcactcact tcgcatctgg cttccggcac accgggtacg
    58801 gtagcaggtt tctcgctggc gctggataaa tacggcacca tgccgctgaa caaagtcgtg
    58861 cagcccgcgt ttaaactggc acgcgatggt tttatcgtta acgacgcgct ggctgacgat
    58921 ctcaaaacct acggtagcga agtgttgccg aatcacgaaa acagtaaagc tatcttctgg
    58981 aaagagggcg agccgctgaa aaagggcgac acgctggtgc aggcgaacct ggcaaagagc
    59041 ctggagatga ttgctgaaaa cggcccggac gaattctata aaggcacgat tgcggaacag
    59101 atcgcccagg agatgcagaa aaacggtggc ttgatcacta aagaagattt agcggcctat
    59161 aaagcggtcg aacgcactcc gataagcggc gattatcgcg ggtatcaggt ttactccatg
    59221 ccaccgccat cctccggcgg gatccatatc gtacaaatcc tcaatattct ggaaaacttc
    59281 gatatgaaga aatacggctt tggcagcgcc gatgcgatgc aaatcatggc agaagcggag
    59341 aaatacgcct acgccgaccg ctcggaatat cttggcgacc cggattttgt caaagtcccg
    59401 tggcaggcac tgaccaataa agcctatgcc aaatccattg ccgatcaaat tgatatcaac
    59461 aaagcgaagc cgtccagtga gattcgccct ggcaagcttg cgccttatga gagtaatcaa
    59521 actacccatt actctgtagt ggataaagac ggtaacgcgg tggcggtgac ctatacgctg
    59581 aacaccacct tcggtacggg cattgtcgcg ggcgagagcg gtattctgct taataatcag
    59641 atggatgatt tctccgccaa accgggcgta ccgaacattt acgggctggt gggcggtgat
    59701 gccaacgccg tcgggccgaa taaacgtccg ctgtcatcga tgtcaccaac cattgtggtg
    59761 aaagacggta aaacttggct ggttaccggt agtcctggcg ggagtcggat tattaccacc
    59821 gtgct
//
LOCUS       DAJVQG010000007        58385 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJVQG010000007 DAJVQG010000000
VERSION     DAJVQG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58385
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.007"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..160
                     /locus_tag="OQM83_000402"
     CDS             <1..160
                     /locus_tag="OQM83_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCS1518755.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(198..1259)
                     /locus_tag="OQM83_000403"
     CDS             complement(198..1259)
                     /locus_tag="OQM83_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCS1518756.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1472..2935
                     /gene="bepA"
                     /locus_tag="OQM83_000404"
     CDS             1472..2935
                     /gene="bepA"
                     /locus_tag="OQM83_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCS1518757.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            2956..3315
                     /gene="arsC"
                     /locus_tag="OQM83_000405"
     CDS             2956..3315
                     /gene="arsC"
                     /locus_tag="OQM83_000405"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCS1518758.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3453..4199)
                     /gene="hda"
                     /locus_tag="OQM83_000406"
     CDS             complement(3453..4199)
                     /gene="hda"
                     /locus_tag="OQM83_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCS1518759.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(4249..5538)
                     /gene="uraA"
                     /locus_tag="OQM83_000407"
     CDS             complement(4249..5538)
                     /gene="uraA"
                     /locus_tag="OQM83_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCS1518760.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5624..6250)
                     /gene="upp"
                     /locus_tag="OQM83_000408"
     CDS             complement(5624..6250)
                     /gene="upp"
                     /locus_tag="OQM83_000408"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCS1518761.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6575..7612
                     /gene="purM"
                     /locus_tag="OQM83_000409"
     CDS             6575..7612
                     /gene="purM"
                     /locus_tag="OQM83_000409"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCS1518762.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7612..8250
                     /gene="purN"
                     /locus_tag="OQM83_000410"
     CDS             7612..8250
                     /gene="purN"
                     /locus_tag="OQM83_000410"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCS1518763.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8421..10487
                     /gene="ppk1"
                     /locus_tag="OQM83_000411"
     CDS             8421..10487
                     /gene="ppk1"
                     /locus_tag="OQM83_000411"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCS1518764.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYVASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10492..12033
                     /gene="ppx"
                     /locus_tag="OQM83_000412"
     CDS             10492..12033
                     /gene="ppx"
                     /locus_tag="OQM83_000412"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCS1518765.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12072..14315)
                     /gene="pdeF"
                     /locus_tag="OQM83_000413"
     CDS             complement(12072..14315)
                     /gene="pdeF"
                     /locus_tag="OQM83_000413"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCS1518766.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(14497..14649)
                     /locus_tag="OQM83_000414"
     CDS             complement(14497..14649)
                     /locus_tag="OQM83_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1518767.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            14667..14858
                     /locus_tag="OQM83_000415"
     CDS             14667..14858
                     /locus_tag="OQM83_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCS1518768.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            15169..15687
                     /locus_tag="OQM83_000416"
     CDS             15169..15687
                     /locus_tag="OQM83_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCS1518769.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            15703..16242
                     /locus_tag="OQM83_000417"
     CDS             15703..16242
                     /locus_tag="OQM83_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCS1518770.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16337..17914)
                     /gene="guaA"
                     /locus_tag="OQM83_000418"
     CDS             complement(16337..17914)
                     /gene="guaA"
                     /locus_tag="OQM83_000418"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCS1518771.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(17983..19449)
                     /gene="guaB"
                     /locus_tag="OQM83_000419"
     CDS             complement(17983..19449)
                     /gene="guaB"
                     /locus_tag="OQM83_000419"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCS1518772.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            19611..20981
                     /gene="xseA"
                     /locus_tag="OQM83_000420"
     CDS             19611..20981
                     /gene="xseA"
                     /locus_tag="OQM83_000420"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCS1518773.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(20978..21193)
                     /locus_tag="OQM83_000421"
     CDS             complement(20978..21193)
                     /locus_tag="OQM83_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCS1518774.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(21262..22734)
                     /gene="der"
                     /locus_tag="OQM83_000422"
     CDS             complement(21262..22734)
                     /gene="der"
                     /locus_tag="OQM83_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCS1518775.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(22852..24030)
                     /gene="bamB"
                     /locus_tag="OQM83_000423"
     CDS             complement(22852..24030)
                     /gene="bamB"
                     /locus_tag="OQM83_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCS1518776.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(24041..24661)
                     /gene="yfgM"
                     /locus_tag="OQM83_000424"
     CDS             complement(24041..24661)
                     /gene="yfgM"
                     /locus_tag="OQM83_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCS1518777.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(24679..25953)
                     /gene="hisS"
                     /locus_tag="OQM83_000425"
     CDS             complement(24679..25953)
                     /gene="hisS"
                     /locus_tag="OQM83_000425"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCS1518778.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(26064..27182)
                     /gene="ispG"
                     /locus_tag="OQM83_000426"
                     /gene_synonym="gcpE"
     CDS             complement(26064..27182)
                     /gene="ispG"
                     /locus_tag="OQM83_000426"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCS1518779.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(27209..28222)
                     /gene="rodZ"
                     /locus_tag="OQM83_000427"
     CDS             complement(27209..28222)
                     /gene="rodZ"
                     /locus_tag="OQM83_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCS1518780.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(28507..29661)
                     /gene="trmG/rlmN"
                     /locus_tag="OQM83_000428"
     CDS             complement(28507..29661)
                     /gene="trmG/rlmN"
                     /locus_tag="OQM83_000428"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCS1518781.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(29811..30242)
                     /gene="ndk"
                     /locus_tag="OQM83_000429"
     CDS             complement(29811..30242)
                     /gene="ndk"
                     /locus_tag="OQM83_000429"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCS1518782.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(30391..32703)
                     /gene="pbpC"
                     /locus_tag="OQM83_000430"
     CDS             complement(30391..32703)
                     /gene="pbpC"
                     /locus_tag="OQM83_000430"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCS1518783.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(32704..37665)
                     /gene="yfhM"
                     /locus_tag="OQM83_000431"
     CDS             complement(32704..37665)
                     /gene="yfhM"
                     /locus_tag="OQM83_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HCS1518784.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            37872..38717
                     /gene="sseA"
                     /locus_tag="OQM83_000432"
     CDS             37872..38717
                     /gene="sseA"
                     /locus_tag="OQM83_000432"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCS1518785.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(39210..39986)
                     /gene="sseB"
                     /locus_tag="OQM83_000433"
     CDS             complement(39210..39986)
                     /gene="sseB"
                     /locus_tag="OQM83_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCS1518786.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESAVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(40128..41411)
                     /gene="pepB"
                     /locus_tag="OQM83_000434"
     CDS             complement(40128..41411)
                     /gene="pepB"
                     /locus_tag="OQM83_000434"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCS1518787.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(41589..41789)
                     /gene="iscX"
                     /locus_tag="OQM83_000435"
     CDS             complement(41589..41789)
                     /gene="iscX"
                     /locus_tag="OQM83_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCS1518788.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(41801..42136)
                     /gene="fdx"
                     /locus_tag="OQM83_000436"
     CDS             complement(41801..42136)
                     /gene="fdx"
                     /locus_tag="OQM83_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCS1518789.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(42138..43988)
                     /gene="hscA"
                     /locus_tag="OQM83_000437"
     CDS             complement(42138..43988)
                     /gene="hscA"
                     /locus_tag="OQM83_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCS1518790.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(44005..44520)
                     /gene="hscB"
                     /locus_tag="OQM83_000438"
     CDS             complement(44005..44520)
                     /gene="hscB"
                     /locus_tag="OQM83_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCS1518791.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(44616..44939)
                     /gene="iscA"
                     /locus_tag="OQM83_000439"
     CDS             complement(44616..44939)
                     /gene="iscA"
                     /locus_tag="OQM83_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCS1518792.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(44956..45342)
                     /gene="iscU"
                     /locus_tag="OQM83_000440"
     CDS             complement(44956..45342)
                     /gene="iscU"
                     /locus_tag="OQM83_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCS1518793.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(45370..46584)
                     /gene="iscS"
                     /locus_tag="OQM83_000441"
     CDS             complement(45370..46584)
                     /gene="iscS"
                     /locus_tag="OQM83_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCS1518794.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(46825..47313)
                     /gene="iscR"
                     /locus_tag="OQM83_000442"
     CDS             complement(46825..47313)
                     /gene="iscR"
                     /locus_tag="OQM83_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCS1518795.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(47614..48351)
                     /gene="trmJ"
                     /locus_tag="OQM83_000443"
     CDS             complement(47614..48351)
                     /gene="trmJ"
                     /locus_tag="OQM83_000443"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCS1518796.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            48470..49273
                     /gene="suhB"
                     /locus_tag="OQM83_000444"
     CDS             48470..49273
                     /gene="suhB"
                     /locus_tag="OQM83_000444"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCS1518797.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            49418..50272
                     /locus_tag="OQM83_000445"
     CDS             49418..50272
                     /locus_tag="OQM83_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1518798.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            50463..51743
                     /gene="csiE"
                     /locus_tag="OQM83_000446"
     CDS             50463..51743
                     /gene="csiE"
                     /locus_tag="OQM83_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCS1518799.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(51735..52874)
                     /locus_tag="OQM83_000447"
     CDS             complement(51735..52874)
                     /locus_tag="OQM83_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCS1518800.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(53034..53924)
                     /gene="hcaR"
                     /locus_tag="OQM83_000448"
     CDS             complement(53034..53924)
                     /gene="hcaR"
                     /locus_tag="OQM83_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCS1518801.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            54060..55421
                     /locus_tag="OQM83_000449"
     CDS             54060..55421
                     /locus_tag="OQM83_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCS1518802.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            55418..55936
                     /locus_tag="OQM83_000450"
     CDS             55418..55936
                     /locus_tag="OQM83_000450"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCS1518803.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            55936..56256
                     /locus_tag="OQM83_000451"
     CDS             55936..56256
                     /locus_tag="OQM83_000451"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCS1518804.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            56253..57065
                     /gene="hcaB"
                     /locus_tag="OQM83_000452"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             56253..57065
                     /gene="hcaB"
                     /locus_tag="OQM83_000452"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCS1518805.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            57075..58277
                     /locus_tag="OQM83_000453"
     CDS             57075..58277
                     /locus_tag="OQM83_000453"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCS1518806.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
BASE COUNT        14271 a        15785 c        14566 g        13763 t
ORIGIN      
        1 ctggcaactg gcgcacagta gcgcagacaa ttttccggca ctgacggtaa gccattttat
       61 taccgccaat ctgctcccgg tgatgctggg taatattatc ggcggtgcag tgctggtgag
      121 tatgtgttat cgggctattt atttacgtca ggaatcctga aaaatcagcc cggcgaaaca
      181 gttcgtcggg ctgaagatta ttcttgcgct atttgcccat cgggccaggc gtggatcacg
      241 gctttgatca gcgtcgccaa tggaatagcg aagaatacgc cccagaatcc ccacaaacca
      301 ccgaagatca ccaccgataa aataatcacc agcggatgca ggttaaccgc ttcggagaac
      361 aacaccggta ctaacagatt accgtccagc gcctgaataa tcagatacac tgcgaagcag
      421 ctccagaatt ccgtgcctgc accaaactgg aatagcgcca cgccaaccac cggaatggtc
      481 accacaaatg cgccaatgta cggaataagt accgagaagc cgaccagcac cgccagcagc
      541 agcgaatagt tcagcccaaa gagcaagaac cccagccaag tggcgatccc caccacgatc
      601 atctccagca ctttgccgcg gatatagttg gtgatttgtt gattcatctc cttccacacc
      661 tgtcctgcca gtccacggtt gcgcggcagc acccgacgaa cggcgttcag catctgctct
      721 ttgtctttca gcaggaagaa gaccatcaat ggtaccagca ccagatagac ggcaatggtc
      781 agcaaaccga ccagcgaggc gagggaaatt ttcaccaccg aatcgcccat ggtcagcatc
      841 cgactgcgca tattttcggc cattgcatca ataatgcccg cgtccattaa tgccggatag
      901 cggcgcggca acgtggcggc aaagtcagaa agcttattga gcatccccgg catatcgcgg
      961 atcaagtaga tgccctgttg ccaggcgata ggcagtacca cgaacgccat cagtagcaat
     1021 ataccgacga aaaccaccaa tacaatcgac gtcgcccagc ggcgggagca gccaatagat
     1081 tgcaggcgca cggttggcca ttccagcaaa taggccagca caatagccac cagcaacgga
     1141 gcaagcaggc cactaaagaa aaagataatg ccaaatccgg caactaaaat aaccagcaag
     1201 gcaatcgctt ccgggtcgct aaaacggcgg cgataccatt gcatcaacat ttcgagcata
     1261 aagaccttcc ctgaacctca agagcgggat tgcgatccgc aattgtatcg aaatgtcaca
     1321 aaaaagactt cgctttttat gacggattca ggaaactgaa aagtcatttg agtgggctaa
     1381 tcttcgccgt tacactcaaa ggcggcgcgg tgggaacaat atttcacagt atcggtcaaa
     1441 tgactatctt cagaaataca ggatagaggt tatgttcagg cagttgaaaa aaaacctggt
     1501 tgcaaccctc attgctgcta tgaccattgg tcaggtagcc ccggcgtttg ccgacagcgc
     1561 agacaccttg ccggatatgg gaacctccgc aggaagcacg ctttccattg gtcaggaaat
     1621 gcagatgggc gactattatg tccgccagct acgcggcagc gcgccgttaa ttaatgaccc
     1681 gctgttaacg caatatatta attcgctggg gatgcgtctg gtttcgcatg ccaattcggt
     1741 taagacaccg tttcattttt ttctgatcaa caacgacgaa attaacgcct ttgctttctt
     1801 tggcggcaac gtggtgctgc actctgccct gttccgttat tccgataacg aaagtcaact
     1861 ggcttcagtt atggcgcacg aaatctccca cgtcacccaa cgtcacctgg cgcgagcgat
     1921 ggaagatcag cagcgcagcg cgccgctgac ctgggtcggc gcgttaggtt ctattttact
     1981 ggcgatggcc agtccgcagg cggggatggc ggcgctgacc ggtacactgg cgggaacgcg
     2041 tcaggggatg atcagtttca cccagcaaaa tgaacaggaa gcggaccgca ttggtattca
     2101 ggtgctgcaa cgctcgggat tcgatccgca ggcgatgcca accttcctcg aaaaattact
     2161 cgatcaggcg cgttactcct cgcgcccgcc ggaaatttta ttgactcacc cgttgccgga
     2221 aagtcgtctg gcagatgccc gcaaccgtgc taatcagatg cgcccgatgg tggtgcagtc
     2281 gtcggaagat ttctatctgg cgaaagcgcg cacactgggg atgtataatt ccggacgtaa
     2341 ccagctcacc agtgatttgc tggatgaatg ggcgaaagga aacgttcgtc agcaacgagc
     2401 ggcgcaatat ggtcgtgctt tacaggcgat ggaagccaat aaatacgacg aggcgcgtaa
     2461 aacgctgcaa ccgttactgg cggcagaacc tggcaacgca tggtatctcg atctggctac
     2521 cgatatcgat cttgggcaaa acaaagccaa tgaggcaatc aatcgcctga aaaatgcccg
     2581 tgatttgcgc accaatccgg tgttgcagct caacctggca aacgcttatc tgcaaggcgg
     2641 tcaaccacaa gaagcggcca atattctgaa tcgctacacc tttaataata aagatgacag
     2701 caacggctgg gatttactgg cacaggcgga agccgcgcta aataaccgcg atcaggagct
     2761 ggctgcgcga gcagaaggtt atgcgctcgc cggacgactc gatcaggcca tttcgctgtt
     2821 gagtagcgcc agttcgcagg tgaaattagg cagccagcaa caagcgcgtt acgatgcgcg
     2881 catcgaccag ttgcgccagc tgcaggaacg ctttaagcct tataccaaga tgtaattcgc
     2941 attaaaggaa gaacaatgac caaacaggta aaaatctacc ataacccacg ctgttcaaag
     3001 agccgggaaa cgctgaacct gctgaaaaaa aacggcgtgg agccagaagt ggtactctac
     3061 cttgagacac ccgccgatgc ggcaacgctg cgcgatttgc tgaaaatgct ggggatgaat
     3121 agcgcccgtg aattgatgcg ccagaaagag gatctttata aagaactgaa cctggctgac
     3181 agttcgctta gcgaagaggc gctgattcag gcaatggtgg ataatccgaa gctgatggag
     3241 cgcccgattg tggtcgcgaa tggcaaagcg cggattggtc gtccaccgga gcaggtactg
     3301 gaaatcgtgg gttaagatgt gccccatcgc tagttgaagc acattgccgg atgcgacgct
     3361 tgccatgtct tatccggcct acgaatacta tggattaact tcgcgtagtt cggataaggc
     3421 gttcgcgccg catccgacaa taaacacctt atctacaact tcagaatttc tttcacaaac
     3481 ggaatggtca gcttacgttg cgcggtaatc gacgcacgat ccaactgatc caacgtcata
     3541 aatagcgtgc gcatttctct gtcgagccgc ttcagcaaga aacgccccac atcttccggc
     3601 agttcaaaac cacgcaaacg cgcgcgtaac tgtagcgcct gcaacttatc ttcatcagaa
     3661 agtggctgca atttgtagat ctgcccccag tcgagtcgcg acgcgagatc cggtaatccc
     3721 agattcaact gccgcggtgg acgatcgccg gtgatcaaca atcgtgtttt gcccgattcc
     3781 agaattcgat tgtagagatc gaaaatcgcc atctcccaca actcatcgcc tgcaatacac
     3841 tcaatgttgt cgatacagac cagcgacaaa tgctccatac cgtcgagcac ttccggaaca
     3901 aaccaggtgc gtttatccag cgggacatag cccaccgcat cgccacgctg cgacaattcc
     3961 gcgcaagccg cgtgcagcag atggctgcgc cccgcgcctt cgcgtgccca gagatagatg
     4021 taaccgctat gttcctgacg cagcacgttt tgcagcgcag ccagtaaaga ggagttatcc
     4081 cccggccaga aacttgcaaa ggtttcgtcg tcaggaagat aaagtggcaa agagagctgt
     4141 gccggtgtgt tcagagatac ctcaaccagg atttcacaaa atcgcgagaa gtttaccaca
     4201 gaatcccatg atgtttgaac cgggcagcaa cactgcccgg tcggtacatt atttgtctgt
     4261 tatatccgcg tcttctgcgt ccagcaccac ttcttcaggg cgcagcacac taatcagttt
     4321 gaagatcaaa cttaagccga taccgacgat ggtcgccagc gccatacctt tcagctccgc
     4381 cgcgccaatg tttaccttcg cgccactgac accgatgatc aaaatcacgg aagtcaggat
     4441 caggttctgt gctttgttgt aatccacttt tgattcgatc aaaacacgaa taccggaagc
     4501 accgatgaca ccataaagca gcagcgaaac gccgcccatc accggcaatg ggatcatctg
     4561 gatagcggca gccagtttac cgacgcagga aagcaggata gcgaaaatcg ccgccccacc
     4621 gataacccag gtactgtaaa cacgggtgat cgccatcacg ccaatgtttt ctccgtaagt
     4681 ggtatttggc gtagagccaa agaagccgga gatcacagtc gacaggccgt tagcaaacat
     4741 cgaacggtgc agacctggat cgcgcagcag atcttttttg acgatattag ccgttactac
     4801 caggtgccct acgtgttcgg caataaccac taacgccgct ggcagaatag tcagaatggc
     4861 aaaccactcg aagcgcggcg tatagagggt tggcagcgca aaccagtgag cattaataat
     4921 cggcgtggta tcgacaattc ccattgcgaa agagagcgcg taccccacca gcacgccaat
     4981 taaaatcggg ataattgcca ggaaaccacg gaacagcacg gaacctaaaa ccgtgaccgc
     5041 cagggtggta atagagatga tgatggtttt ggagtctggc gtttgccctt cagccgggag
     5101 taaacccgcc ataccggcag ctacgcccgc cagctccaga ccgatgacgg caacgattgc
     5161 gcccattgcc gcaggtggaa acagcacgtc cagccagccg gtccccgctt tcttcacgat
     5221 aaaagaaacc aggcagaaca gcacgccgca cataataaag ccgcccagcg cgacttcata
     5281 ccctaacggc aacagtaaca ataccggtga aataaaggca aagctggaac caagataagc
     5341 cggaattttc cctttacaga tgaagagata cagcagcgtt ccaataccgt taaataacag
     5401 tacagtcgcc gggttaatat gaaataagac gggcaccagg acggttgcac caaacatggc
     5461 gaacaaatgt tgcaaactaa gcgggattgt ctgtaaaagt ggcggtcttt cactcacccc
     5521 gatagcacgg cgcgtcatag tattatcctc tgtattatgt gttataggcg ctttactcaa
     5581 aaaaaagccg actcttaaag tcggctttaa ttatttttat tctttatttc gtaccaaaga
     5641 ttttgtcacc ggcatcgccg aggcccggaa taatgtatcc gtgctcgttc agtccctggt
     5701 caatcgatgc ggtatacagt tcgacgtccg ggtgcgcttt ttccagcgca gcgatacctt
     5761 ctggcgcagc taccagcacc agaactttga tgctgctgca gcccgctttt ttcagcaggt
     5821 cgatggtcgc gataacggaa ccaccggttg ccagcattgg gtcaacgatc agcgccatac
     5881 gctcatcgat gttagaaacc agtttctgga agtacggtac cggctccagc gtttcttcat
     5941 tacggtacat accgacaacg ctgatgcgcg cgctcggaac attttccagc acaccgtcca
     6001 tcatccccag accggcacgc agaattggca caacggtaat tttcttacct ttgatctggt
     6061 cgatttctac cgggccgttc cagccttcga tagttacttt ttccgtttcg aggtcggcgg
     6121 tcgcttcgta agtcagcagg ctacccactt cggaagcgag ttcgcgaaag cgcttggtgc
     6181 tgatatcttg ctcacgcatc agtcccagct tgtgtttgac gagtgggtgt ttgacttcca
     6241 cgatcttcat actctttctc ctttgagggg cagccacaaa aaaaatcgac ggattatacc
     6301 tcctttcttc aaggcggcaa tattcttttc gttgacttta gtcaaaatga taacggtttg
     6361 agataaagtt attttatatt caaatggtta tgaaagaaga ttattccatc cgaaaactaa
     6421 cctttgccct ggcacaagtc ttctttcgcc gcgcgtctgg ggaaaagacg tgcaaaaagg
     6481 ttgtgcaaag cagtctcgaa aacgtttgct ttccctgtta gaattgcgcc gaattttatt
     6541 tttctaccgc aagtaacgcg tggggaccca agcagtgacc gataaaacct ctcttagcta
     6601 caaagatgcc ggtgttgata ttgacgcggg taatgctctg gttggaagaa tcaaaggcgt
     6661 agtgaagaaa acgcgtcgtc cggaagtgat gggcggtctg ggcggcttcg gtgcgctgtg
     6721 tgcattgccg caaaaatatc gtgaacccgt gctggtttcc ggcactgacg gcgtaggtac
     6781 caagctgcgt ctggcaatgg acttaaaacg tcacgacacc attggtattg atctggtcgc
     6841 catgtgcgtt aatgacctgg tggtgcaagg tgcagaaccg ctgtttttcc tcgactatta
     6901 cgcaaccgga aaactggatg ttgataccgc ttcagcggtg atcagcggta tcgcggaagg
     6961 atgtctgcaa tcgggctgtt cactggtggg tggcgaaacg gcagaaatgc cggggatgta
     7021 tcacggtgag gattacgatg tcgcgggttt ctgcgttggc gtggtagaaa aatcagaaat
     7081 catcgacggc tctaaagtca gcgacggcga tgtgctgatt gcactcggtt ccagcggtcc
     7141 gcactcgaac ggctattcgc tggtgcgcaa aattcttgaa gtcagcggtt gtgatccgca
     7201 aaccaccgaa cttgatggta agccattagc cgatcatctg ctggcaccga cccgcattta
     7261 cgtgaagtca gtgctggagt tgattgaaaa ggtcgatgtg catgccattg cgcacctgac
     7321 cggcggcggc ttctgggaaa acattccgcg cgtattgcca gataatactc aggcagtgat
     7381 tgatgaatct tcctggcagt ggccggaagt gttcaactgg ctgcaaacgg ctggtaacgt
     7441 tgagcgccat gaaatgtatc gcaccttcaa ctgcggcgtc gggatgatta tcgccctgcc
     7501 tgctccggaa gtggacaaag ccctcgccct gctaaatgcc aacggtgaaa acgcgtggaa
     7561 aatcggtatc atcaaagcct ctgattccga acaacgcgtg gttatcgaat aatgaatatt
     7621 gtggtgctta tttccggcaa cggaagtaat ttacaggcaa ttattgacgc ctgtaaaacc
     7681 aacaaaatta aaggcaccat acgggcagtt ttcagcaata aggccgacgc gttcggcctt
     7741 gaacgcgccc gccaggcggg tattgcaacg catacgctca tcgccagcgc gtttgacagt
     7801 cgtgaagcct atgaccggga gttgattcat gaaatcgaca tgtacgcacc cgatgtggtc
     7861 gtgctggctg gttttatgcg cattctcagc ccggcgtttg tctcccacta tgccgggcgt
     7921 ttgctgaaca ttcacccttc tctgctgccg aaatatcccg gattacacac ccatcgtcag
     7981 gcgctggaaa atggtgatga agagcacggt acatcggtgc atttcgtcac cgatgaactg
     8041 gacggtggcc cggttatttt acaggcgaaa gtcccggtat ttgctggtga tacggaagat
     8101 gacatcaccg cccgcgtgca aacccaggaa cacgctattt atccactggt gattagctgg
     8161 tttgccgatg gtcgtctgaa aatgcacgaa aacgccgcgt ggctggatgg tcaacgtctg
     8221 ccgccgcagg gctacgctgc cgacgagtaa tgccccgtag ttaaagcgcc agctctgccg
     8281 ctggcgtttt tcaattcacc tgtaaatcgc aagctccagc agtttttttc ccccttttct
     8341 ggcatagttg gacatctgcc aatattgctc gccataatat ccaggcagtg tcccgtgaat
     8401 aaaacggagt aaaagtggta atgggtcagg aaaagctata catcgaaaaa gagctcagtt
     8461 ggttatcgtt caatgaacgc gtgcttcagg aagcggcgga caaatctaac ccgctgattg
     8521 aaaggatgcg tttcctgggg atctattcca ataaccttga tgagttctat aaagtccgct
     8581 tcgctgaact gaagcgacgc atcattatta gcgaagaaca aggctccaac tctcattccc
     8641 gccatttact gggcaaaatt cagtcccggg tgctgaaagc cgatcaggaa ttcgacggcc
     8701 tctacaacga gctgctgctg gagatggcgc gtaaccagat cttcctgatt aatgaacgcc
     8761 agctctccgt caatcaacaa aactggctgc gtcattattt taagcagtat ctgcgtcagc
     8821 acattacgcc gattttaatc aatcctgaca ctgacttagt gcagttcctg aaagatgatt
     8881 acacctatct ggcggtggaa attatccgtg gcgataccat ccgttacgcg ctgctggaga
     8941 tcccatcaga taaagtgccg cgctttgtga atttaccgcc agaagcaccg cgtcgacgca
     9001 agccgatgat tcttctggat aacattctgc gttactgcct tgatgatatt ttcaaaggct
     9061 tctttgatta tgacgcgctg aatgcctatt caatgaagat gacccgcgat gccgaatacg
     9121 atttagtgca tgagatggaa gccagcctga tggagttgat gtcttccagt ctcaagcagc
     9181 gtttaactgc tgagccggtg cgttttgttt atcagcgcga tatgcccaat gcgctggttg
     9241 aagtgttacg cgaaaaactg actatttccc gctacgactc catcgtcccc ggcggtcgtt
     9301 atcataattt taaagacttt attaatttcc ccaatgtcgg caaagccaat ctggtgaaca
     9361 aaccactgcc gcgtttacgc catatttggt ttgataaagc ccagttccgc aatggttttg
     9421 atgccattcg cgaacgcgat gtgttgctct attatcctta tcacaccttt gagcatgtgc
     9481 tggaactgct gcgtcaggct tcgttcgacc cgagcgtact ggcgattaaa attaacattt
     9541 accgtgtggc aaaagattca cgtatcatcg actcgatgat ccacgctgcg cacaacggca
     9601 agaaagtgac cgtggtggtt gagttacagg cgcgtttcga cgaagaagcc aacattcact
     9661 gggcgaagcg cctgaccgaa gcaggcgtgc acgttatctt ctctgcgccg gggctgaaaa
     9721 ttcacgccaa actgttcctg atttcacgta aagaaaacgg tgaagtggtc cgttacgcac
     9781 acatcgggac cgggaacttt aacgaaaaaa ccgcgcgtct ttatactgac tattcgttgc
     9841 tgaccgcaga tgcgcgtatc accaacgaag tacggcgggt atttaacttt attgaaaacc
     9901 cataccgccc ggtgacattt gattatttaa tggtgtcacc gcaaaactct cgccgtctgt
     9961 tatatgaaat ggtagaccgc gaaatcgcca acgcgcagca agggctgccc agtggtatca
    10021 ccctgaagct aaataacctt gtcgataaag gcctggttga tcgtctgtat gtggcctcca
    10081 gctccggcgt accggttaat ctgctggttc gcggaatgtg ttcgctgatc cccaatctgg
    10141 aaggcattag cgacaacatt cgtgccatca gtattgttga ccgttacctt gaacatgacc
    10201 gggtttatat ttttgaaaat ggcggcgata aaaaggtcta cctttcttcc gccgactgga
    10261 tgacgcgcaa tattgattat cgtattgaag tggcgacacc gctgctcgat ccgcgcctga
    10321 agcagcgggt gctggacatc atcgacatat tgttcagcga tacggtcaaa gctcgttata
    10381 tcgataaaga actcagtaat cgctacgttc cccgcggcaa tcgccgcaaa gtacgggcgc
    10441 agttggcgat ttatgactac atcaaatcac tcgaacaacc tgaataaccc tatgccaata
    10501 cacgataaat cccctcgtcc gcaggagttt gctgcggtcg atcttggttc aaacagtttt
    10561 cacatggtca tagcccgtgt ggtagatggt gccatgcaga ttattggccg cctgaaacag
    10621 cgggtgcatc tggcggacgg cctggggcca gataatatgt tgagtgaaga ggcaatgacg
    10681 cgcggtttga actgtctgtc gctgtttgcc gaacggctac aagggttttc tcctgccagc
    10741 gtctgtatag tcggcaccca tacgctgcgt caggcgctga acgccactga ctttctgaaa
    10801 cgcgcggaaa aggtcattcc ctacccgatt gaaattattt ccggtaatga agaagcccgt
    10861 ctgattttta tgggcgtgga acatacccaa ccggagaaag gtcgcaaact ggttattgat
    10921 attggcggcg gatctacgga actggtgatt ggtgaaaatt tcgaaccgat tctcgttgaa
    10981 agccgccgga tgggttgtgt cagctttgcc cagctttact tccctggcgg ggtcatcaat
    11041 aaagagaatt ttcagcgcgc tcgtatggcg gcagcacaaa aactggaaac tttaacctgg
    11101 caattccgta ttcagggctg gaacgtggca atgggcgctt ccggtaccat aaaagccgcc
    11161 catgaagtgt taatggaaat gggcgagaaa gacgggataa ttaccccgga acgtctggaa
    11221 aaactggtaa aagaagtttt acgtcaccgt aatttcgcat cgctgagttt accgggtctt
    11281 tccgaagagc ggaaaacagt cttcgttcct ggactggcga ttttatgcgg tgtgtttgat
    11341 gctttagcca tccgtgaact gcgcctttct gacggggcgt tacgcgaagg cgtactgtac
    11401 gaaatggaag gacgtttccg tcatcaggat gtgcgtagtc gcaccgccag cagcctcgcc
    11461 aaccagtatc acatcgacag cgaacaggcc cgacgggtgc tggataccac tatgcaaatg
    11521 tacgaacagt ggcgggaaca gcaaccgaag ctggcgcatc cgcaactgga ggcgctactg
    11581 cgatgggccg ccatgctgca tgaggtcggg ttgaatatca accacagcgg tttgcatcgc
    11641 cactccgctt atattctgca aaacagtgac ttgccgggtt ttaatcagga acagcagctg
    11701 atgatggcga cactggtgcg ctatcaccgt aaagcgatta aactcgacga tctgccgcgc
    11761 tttaccttgt ttaagaagaa acagttcctg ccactgattc agctattgcg ccttggcgta
    11821 ttactcaaca atcaacgtca ggcaaccacc acaccgccaa cattgacact gattactgat
    11881 gacagtcact ggacactgcg tttcccgcat gactggttta gtcagaatgc gttggtactg
    11941 cttgatctgg aaaaggagca agaatactgg gaaggcgtgg ctggctggcg gttgaaaatt
    12001 gaagaagaaa gtacacctga aatcgccgct taatgctttg cgggccgacg agaaatgtcg
    12061 gcccgcatta ttcaggcact ttcgcgaatg ggttcgattt cattcaacgt atcaattaac
    12121 ggctgcggct taccaataag ataaccctgc atataatcga tccccaaaga gagcaccgcc
    12181 tcgcggatct cttcgttttc aacgtactct gccactaccc gcattttctt cattcgcgcc
    12241 agatggcaaa tcgacgccac aatctgataa tccagactat ttgacacaat attgcggata
    12301 aagctgccgt caattttaag cagatcggca ttcacatttt taagccgtgc atagctggcg
    12361 tagccggtgc cgaaatcatc aatcgcaatc tggcagccta attcctgaag atgctgcaag
    12421 gtgatttgcg cctgcttaac attggtcaga gcattacttt cggtgacttc aaaaataagt
    12481 tgccacgctt caatctgata tttagccagc aactgactga cttcaacagg aaaacgagcc
    12541 tgacataccg aggttggaga cagattaata gcaaaacggt gagcgggcat cttcgctctg
    12601 ttttcagcca taaattgcag cgtatgctca atgacccaca tgtcgatact cgacgataaa
    12661 ccaaattcgt gcgcgaccgg caagaagcta tcggggacga tcagttcatc attctcacct
    12721 ttcatgcgca gaagaatttc atggtaaaca tcaccacgca taccggtaat cggctgggcc
    12781 atcaggaaaa aatggttgtg ttccagcgcc tgctgtagtc gattcatcat cgcgacttta
    12841 tctttcaatt cgcgttgcaa atacattgcc ccgcgacgct gcatattttc cggggcgttg
    12901 gtcacgatgg aaagttcggc gaccgtattt agctctccca gcagcaggta gatatgattc
    12961 actggcgagc gcacatagca gtaactgacg ccaatctgcg gttgcatcgg catcccatcc
    13021 caaaagaaac gaaattgctt gagatgacta tccagtgcgg taatgcgctc ctggtgcgat
    13081 tctgtattca gtcgcagcgc gagatcgtta cccgaaagct gataaacatc ttcacccggt
    13141 tccagcaagg gtgacagcca gtgagaaagt ttttgcttgt attgaatccg cagcatgatg
    13201 ccatagttct taaccagcat ttccatgcca gggatgcgta aataacaaag cgcagaccag
    13261 ggagcatcac gcaacgcgcg attcagggcg cgaacatttg gcagatgaac caccgggtcc
    13321 atatacgcaa gccgctgaat gcgtctgact accgctctct gacgggttgc cagtacagcc
    13381 atataattga caataaaaga gaataccaga taactggagg aggttatggt cagctgcgtg
    13441 gtatagccag gataaatggg aatgtaattt tgatagctgt ggatgctgat catcaacacg
    13501 accgcccaga gcagcgaaat cagcttataa ccatagcgca tcgctcccca catcatcagg
    13561 ggcagcaata atgacaaggt ataattggtg ctaaaaattg tgcttttttc atttaacggc
    13621 atgcataaca gcaacaataa agcacctaat gccagtagcc agagcgcgaa ctcttttttg
    13681 gtgactttgg catcaacctg ctgttttaat tgcgaataat agctacgtaa ataaaatgga
    13741 tttcgcacta cccgaatgat gaagtagcac agcgggacac cgatcagatt acccaccagt
    13801 aaggcctgat aattgattaa agtcccgagg ttaaaaggca tgacaccgac cagattttct
    13861 ctgctcgcca gtaatcctac aaatgcagca aactggaaaa gtatcagaaa cagcgttgca
    13921 ggaaacacaa tctgccagaa tatacgctgg gaaattaaac gggtatcacc atgtgaaacg
    13981 ttgttacgcc ggggagtaaa gaccctgtaa ccgccccaac agagaatgat aatgataata
    14041 aaattagccg ttaatgatag cgtttcgtaa aaacccagct ctgcatattt gcggacaaaa
    14101 atccctaagg cgatccctgg caatgccgcc cagctgaaaa acatcatcat gctgatcatc
    14161 agtgccagag gcagataaaa gagaaaaacc tcaccggaag aaatatgcgc gaaagtatta
    14221 atgtgggcga aaatgggcaa gattaaagaa ggcaggaaca gcggaagccc ccaccattta
    14281 tcacgcattt ttatataagt tgcattcagt ttcatagatg ctcagcagaa tcccccacat
    14341 cctgaaggag gtgtattcag acaggcatcc cacctgactt cgaatgatga ttattcatca
    14401 ctatagagag cattgattct aagtgtcata tgaaagttcc aattgatata tatcaaacaa
    14461 aataaccctg attaatgaat tattacgttt atcatgttaa ttcatcatta ttacatcatc
    14521 attgtaaata attaaattaa cttccataac attaaaatat gtatccactg acgttttttt
    14581 acataacgaa gaattgacca ttttgtcctg ttgtgcctta atgtaagtac cgtccacagc
    14641 gtgggacata cttcaaggaa ccttttgtga gtcaggcaac cagtatgcga aaacgacacc
    14701 gatttaacag tcgcatgacc cgtatcgtac tgctcatcag ctttatcttc ttctttggcc
    14761 gttttatcta ctcgtccgtc ggtgcctggc agcaccatca gagcaaaaaa gaagctcagc
    14821 aatccacact ctccgtcgaa tcaccggtac aacgttagcg gttaccttct tcactttcac
    14881 agaacataac ggcacttcgc tgtcggatgc ttttgctctt tgggattatc aaagcggcag
    14941 atattctttc atcttaaatt ttacgtcttt gtcctgactg atgtttatcc tgtttggctg
    15001 cggaataaat ataaaattaa tatatatgtt gtaatgatat atttttataa attattccct
    15061 gcgtgaattt taataaattt aatctatccc tttatacgca atacatttac tttcctcttt
    15121 tgatgatctt aaatgtctta tttttcgtaa tgtgtataac aaggaatagt gatgaaattt
    15181 aaaaaatgtc ttctgcctgt ggcaatgtta gcgtcattca ctctggcagg atgccagtca
    15241 aatgctgacg atcatgccgc cgatgtttat caaaccgatc aactgaatac caaacaagaa
    15301 actaaaaccg ttaatattat ttccattctt cccgcaaaag ttgccgtaga caactcccaa
    15361 aataaacgga acgcacaagc cttcggcgcg cttattggcg cagtcgctgg cggtgttatc
    15421 ggccacaacg tcggttctgg cagcaattcc ggaacgacgg caggggcagt tggcggcgga
    15481 gctgtaggcg cggcagcggg ttctatggtg aatgataaaa ccttagtgga aggtgtttct
    15541 ttaacatata aggaaggcac caaagtgtat acctccaccc aggtgggtaa agagtgccag
    15601 tttacgacag gtttagccgt tgttattacc acaacgtata acgaaacgcg tattcagcca
    15661 aatactaaat gtcctgaaaa gagctaataa tcaggaggag tcatgaagaa agtttttctt
    15721 tgcgccatct tagcctcctt aagctatccg gctatcgcct catcattgca ggatcaactc
    15781 tctgctgtcg cagaagcgga acagcaaggt aaaaatgaag agcaaaggca gcatgacgaa
    15841 tgggtcgcgg agcgcaacag ggaaatccag caagagaagc aacgtcgcgc aaatgcccag
    15901 gccgccgcta acaaaagagc ggcaacggca gcggcaaata agaaagctcg tcaggataaa
    15961 ctggacgccg aagccactgc ggacaaaaaa cgcgatcaaa gttatgaaga tgagctacgc
    16021 agcttagaga ttcagaaaca aaaactgacg ctggcgaaag aagaagcccg cgttaagcga
    16081 gaaaacgaat ttatcgatca ggaactgaag cacaaagctg cgcaaaccga tgtggtgcaa
    16141 tctgaagctg acgccaacag aaatatgact gaaggcggtc gcgatctgat gaaaagcgtg
    16201 ggcaaagcag aagagaacaa atcggacagc tggtttaatt aatcgatgtt agtaacttca
    16261 agcctatgat tcttgaagat aaaaaaccct ctgtagtaac agagggtttt gttcattcat
    16321 agtgcaggga ttaaaatcat tcccactcaa tggtagctgg cggcttgccg ctgatgtcat
    16381 acaccacgcg ggaaataccg ttcacttcat tgataatgcg gttggaaacg cgaccgagga
    16441 aatcgtacgg cagacgcgcc cagtgtgcgg tcataaagtc gatggtttcg acagcacgca
    16501 gagagacaac ccagtcatac ttacgaccat cgcccattac gccaacggaa cgtaccggca
    16561 ggaacacagt gaacgcctgg ctgactttgt cgtacaggtc cgctttacgc agttcttcaa
    16621 tgaagatggc gtcagcacgg cgcagcaggt cacagtactc tttcttcact tcacccagca
    16681 cacgaacgcc aaggcctggt cccgggaatg ggtgacggta caacatgtcg tacggcaggc
    16741 ccagctccag accaatctta cgcacttcgt ctttgaacag ctctttcagc ggttcaacca
    16801 ggcccatctt catctctttc ggcaggccgc ccacgttgtg gtgagatttg atgacgtgtg
    16861 ctttaccggt tgcagacgcc gcagattcga taacgtcagg gtagatggtg ccctgcgcca
    16921 gccacttcac gtcttccagt ttcagcgctt cttcatcgaa tacttcaacg aaaacgcgac
    16981 cgatgatttt acgttttgct tccggatcgt tttcgccagc cagcgctgac aggaagcgat
    17041 cttctgccgg aacgtgaaca atgttcagac caaagtgatc gccaaacata tccagaacct
    17101 gctctgcttc gttgagacgc agcaggccgt tgtcgacgaa tacgcaagtc aggtttttac
    17161 cgatagcgcg gtgcagtagc attgcggtta cggaggaatc cacgccacca gagaggccga
    17221 ggatgacttt atcgtcgcct acctgctcac ggatgcgggc tacagcatcg tcgataattt
    17281 tcgctggcgt ccacagggct tcacactggc agatatcacg cacaaaacgc tccagcatgc
    17341 gcataccctg gcgggtgtga gtcacttccg ggtggaactg tacgccatag aagcgttttt
    17401 cttcgttagc cataatggca aacgggcagc tttcggtgct ggctacggtg acgaagtcgg
    17461 acgggatagc ggtaacttta tcgccgtggc tcatccagac atcgagcagc ggtttaccgt
    17521 ctgcggtcag cgcatcttcg ataccgcgaa ccagtgcact gtcgtttacg acttcaacct
    17581 gcgcgtagcc aaattcacgt tcgttagagg cttcaacgtg accgcccaac tgcattgcca
    17641 tggtctgcat gccatagcaa acgccgaata ccggtacgcc tgcttcaaag acatactgcg
    17701 gcgcacgcgg gctgttttct tcagtagtac tttccgggcc gccggaaaga ataatgccgc
    17761 ttggattgaa gtcacgaatt tgtgcttctg tcacatccca cgcccacagt tcgcagtaaa
    17821 cacccagctc acgcacgcgg cgcgcaacca gttgagtgta ctgagaaccg aagtctagaa
    17881 tgaggatgcg atgcttatga atgttttccg tcattgacgc ttattccgag gcaagtgaaa
    17941 cagataatat aaatcgcccg acatgaagtc gggcgaagag aatcaggagc ccagacggta
    18001 gttcggggac tctttagtaa tggtcacgtc gtgaacgtgg ctttcctgaa tgcccgcacc
    18061 gctgatacgt acaaactccg ctttagtacg cagttcgtcg atagtaccac agccggtcag
    18121 acccatacag gagcgcaggc cgcccatctg ctggtgaatg atctctttca ggcgaccttt
    18181 ataagctacg cgaccttcga taccttccgg caccagtttg tcggcagcgt tatcgctctg
    18241 gaaataacgg tcagaggaac ctttggacat cgcgcccagg gaacccatac cacggtaaga
    18301 tttgtaagaa cggccctggt agagttcgat ttcacccgga gattcttcag tacccgccag
    18361 catggaacct accatcaccg cgcttgcgcc agcggcgata gctttggcga tgtcgccgga
    18421 gaagcgaata ccaccatctg cgataaccgg aataccggtg ccttccaggg cttcaactgc
    18481 gtcagcaacg gcagtgatct gcggaacacc tacgccagta acgatacgag tcgtacagat
    18541 agaaccaggg ccgataccga ctttaaccgc gctgcaacca gcttctgcca aagcgcgtgc
    18601 acctgcagct gttgccacgt tgccgccgat gatttgcaga tccggatatt tagcacgggt
    18661 ttcacggata cgctgcaaaa cgccttctga gtgaccgtgg gaggagtcga tcagcagaac
    18721 gtcaacgcca gcggcaacca gcgcgtcaac acgctcttcg ttacccgcac ctgcgccaac
    18781 tgcagcacca acacgcagac ggccttgctc gtctttacag gcgttcggtt tacgttccgc
    18841 tttctggaag tctttcacgg tgatcatgcc gatcaggtgg aattcgtcat caaccaccag
    18901 cgctttttca acgcgttttt cgtgcatttt tgccagcacc acttcacggg cttcaccttc
    18961 acgcacggtg accagacgct ctttcggcgt catgtaaacg ctaaccggct ggttcaggtc
    19021 ggtaacaaaa cgcacgtcac gaccggtgat aatacccacc agttcgtttt cttcggtaac
    19081 gaccggatag cccgcaaaac cgttacgctc ggtcagttct ttcacttcgc gcagcgtcgt
    19141 ggttggcaga acagtctgcg gatcagtcac cacaccagat tcgtgttttt tcacacggcg
    19201 aacttcttct gcctggcgtt caatggacat gtttttgtgg ataaagccga taccgccttc
    19261 ctgagccaga gcaatagcca ggcgcgcttc cgttacggta tccattgctg cggaaagcat
    19321 agggatattc agacgaatag ttttcgtcag ctgggtgctg aggtcagcag tattcggcag
    19381 aacggtagaa tgagcaggaa cgaggagaac gtcgtcaaac gtcagagctt ctttagcgat
    19441 acgtagcatg ggcaatatct cgaccagagt ggttaataaa tattgccgcg gcattataca
    19501 gagcgtaacc gattgcatct accccttttt gcaaaaaatg cttgctatcc ccgaagggcg
    19561 ggttactatc gactgaataa cctgctgatt tagaatttga tctcgctcac atgttacctt
    19621 ctcaatcccc tgcaattttt accgttagtc gcctgaatca aacggttcgt ctgctgcttg
    19681 agcatgagat gggacaggtt tggatcagcg gcgaaatctc taatttcaca caaccggctt
    19741 ccggtcactg gtactttaca ctcaaagacg acaccgccca ggtacgctgc gcgatgttcc
    19801 gcaacagcaa ccgccgggtg accttccgtc cacagcatgg acaacaagtt ttagttcgcg
    19861 ccaatattac gctctacgag ccgcgcggtg actaccagat aatcgttgag agtatgcagc
    19921 cggccggtga agggctgctg caacagaagt acgaacagct caaagcgaag ttgcaggctg
    19981 aaggtttgtt cgatcagcaa tacaaaaaac cacttccctc ccctgcgcat tgcgttggtg
    20041 tgatcacctc aaaaaccggt gctgcgctac atgatatttt gcatgtgtta aaacgtcgcg
    20101 atccttctct accggtgatc atctacccca ccgccgttca gggcgatgac gcaccggggc
    20161 aaattgttcg cgccattgaa ctggcgaatc agcgcaatga gtgcgacgtg ttgatcgttg
    20221 ggcgcggcgg cggttcgctg gaagatttat ggagttttaa cgacgaacgc gtagcgcggg
    20281 cgatttttgc cagccgcatt ccggtcgtca gcgccgtcgg gcatgagacg gatgtgacca
    20341 ttgccgattt tgttgccgat ctgcgtgcgc caacgccgtc tgccgccgct gaagtagtga
    20401 gccgtaatca gcaagagtta ctgcgccagg tgcaatcgac ccgtcaacgg ctggagatgg
    20461 cgatggatta ttatctcgcc aaccgcacgc gtcgctttac gcagatccat caccgattac
    20521 agcaacagca tccgcagctc cggctggcac gccagcaaac catgcttgag cgcctgcaaa
    20581 agcggatgag ctttgcgctg gaaaatcaac ttaagcgtac cgggcaacag cagcagcggt
    20641 taacacagcg gctgaatcag caaaatccac agccgaagat tcatcgcgcg caaacgcgca
    20701 ttcagcaact ggaatatcgt ttagcagaaa ccctgcgcgt acagcttagc gccacgcgtg
    20761 aacgtttcgg taatgcagta acgcacctcg aagccgtaag cccactgtca acgctggcgc
    20821 gtggatacag cgttactact gctactgacg gcaatgtact gaaaaaagtg aagcaagtta
    20881 aagcgggtga aatgctaacc acacgtctgg aagacggctg gatagaaagt gaagttaaaa
    20941 acatccagcc agtaaaaaaa tcgcgtaaaa aggtgcatta agccagcacg aattcaacgc
    21001 gttttttcga tatcaagcca tgcccgtgct gacagaagta atctactgca ccgcaggctt
    21061 ttaacacctg caacggttga tggcagtctg gacaaagagc tttcatttca ataatttccc
    21121 cgcaggatgg gcaacgcgca tggccgttat cctgatcaag cacatgctga cattgtggac
    21181 aatgcagttc catatgaatt cctccggtag ccatccctct acattcatag agggaatggc
    21241 agataaaata cttacggata attatttatt tttcttgatg tgcttcatca gacgcttacg
    21301 tttacgcatc tgggttggcg tcagggtgtt acgcttgttc gcatacgggt tttccccttc
    21361 tttgaactga atacgaatcg gcgatcccat tacgtccagc gatttgcgga agtagttcat
    21421 caagtagcgc ttgtaggaat caggcaggtc tttcacctga ttaccgtgaa tcaccacaat
    21481 cggcgggtta taaccaccag cgtgggcata tttcagcttc acacgacgac cgcgtaccag
    21541 cggcggttgg tgatcttcaa cagccatcgt catgatgcgc gtcagcatag aggtccccac
    21601 acgacgggtg gagctgtcat acgcttcacg tactgattca aacaagttac caacaccact
    21661 gccgtgcaag gcagagataa agtgcacacg agcaaaatcg ataaagccca gacggaagtc
    21721 cagcgtttct ttcacctgct ctttcacttc ctggctcagg ccatcccact tattcaccac
    21781 aatgacaagt gagcgcccac tattgagaat aaagcccagc agcgagagat cctgatcgga
    21841 aataccttcg cgcgcatcaa tcactaacat caccacgttg gtatcttcaa ttgcctgcaa
    21901 cgttttgatt acggagaatt tctctacagc atcggtgatt ttgccgcgtt tacgtacgcc
    21961 agcggtgtca atgagcacat actcacgtcc atcgcgttcc attggaatgt agatactgtc
    22021 acgcgtcgtg ccaggcatgt cataaacaac aacgcgctct tcaccaagaa tacggttagt
    22081 gagtgtagac ttacctacgt tcggacgacc cacaatcgcc agtttgatcg gcagactttg
    22141 cgggtcgaag tcgtcttcct cttcttcttc gccgttctct tccgcttcaa attgcgccca
    22201 gtattcagcg tcttcgtcga cttcctcttg cggtgcgaga tcttccatcc acggcagcag
    22261 cacacgctcc agcagactta atacgccacg accgtgagat gcggcgatcg ggtagatttc
    22321 acctaaacca agcgagtaga aatcaaccac tgcctgatcg ggatccagac cgtcagtttt
    22381 gtttgccacc aggaaggtcg gtttttcacg agagcgcaga tgtttggcaa tcgcttcatc
    22441 tgccggcatc aagcccgcgc gcgcatccac cataaacagt acgacgtccg cttcttcaat
    22501 cgccagcagc gactgttccg ccatgcgggt ttctacaccg tcttctgtgc catcaatccc
    22561 gccggtatca atacagataa actcacggcc ttcaatttcc gcacgaccgt acttacggtc
    22621 acgagtcaga cccgggaaat ccgcaaccag cgcatctcgg gtgcgagtta gacggttaaa
    22681 taacgtggat tttcctacgt tagggcgccc gacaagcgcg accacaggta ccatgtttaa
    22741 agcctcattt ttataaatca tcagacaacg cacgctatat tcgcgtcgtt gttaaaaaca
    22801 ggaaaacggc ccctgtccag gagccgtttt caaagtgaac gacagagacg attaacgtgt
    22861 aatagagtac acggttccgt cttttgcctg gatcagcagt ttgccgtcag cggcaaccgg
    22921 ttcagtctgg aaaccggaac tatcaacttt ttgctgggca acgaaacgac catcttcgac
    22981 gttaatccag tgcagataac cttcactgtc acctaccacc aggttgccat tatacagcac
    23041 cggcgaagtc agcaggcgat gcagcagatc gctttgtgtc cacagcgtaa cgccgccatc
    23101 aatggtcaac gccatcaccc ggtcattttg atcgaccaga tagatgcgat tgccgtcgac
    23161 gatgaaatca ttcaccgaac ccagttcgcg tttccacata atctgaccac tgcgcagatc
    23221 aagcgccgtc aggttaccat tataggccag agcgaaaaca acgccgttaa caacgacggg
    23281 agtcgtgtca acatcgctca gacggtcaat ttcggtagaa ccggtcgcct gggaaatacg
    23341 ctgctgccaa atcatctggc cctgttccat cagcactgcg ctgacgcgac cattatcgcc
    23401 ccccacgacg gccgcaccaa aagccgttgc cggcgcagac tcgccacgca aagagagcga
    23461 aggcatatcg aggttaactg tccatttgac agcgccgtca gcttcgttca gcgcttgtaa
    23521 ctgaccgtta ctagtgtgga ttaacaccag accgtcgctg accaccgggc gagaaagtgc
    23581 ttcacccgcg actttagttt gccatgccac agtaccatcg ctggtattca gcgcgtaaac
    23641 ctgcgccttt tcgctgccaa tgtagacatg cccaccagac acggtcacac cgccagaaag
    23701 taatgcaggc tctttagaga accagccatc tttctcggcc aggctgacag accagatttc
    23761 tttgccatca tccgcattca gcgcttttac caaaccagcg cggtccgctg catagacaac
    23821 gttgtccgcc agtgccggat gaagattgga atagaagttg ccaatgccgc taccaacgga
    23881 agtgctccac gccgtggtcg gcgtaaactg gttttcaacg gttggcaatg gggacatctt
    23941 taccacatct tcttcgctgt taaacagcga acagccgctt aaaagggtaa cggaaagcag
    24001 tcctggcagc agtaatttac gcaattgcat cgggtccctc tcagatggac aaattattaa
    24061 ttttcatctg catcatttcg ctcagtgccg gagtaacatc gcttttcacg cctgcttccc
    24121 atgcactacg cgcaccttgc ttatcacctt tgctcaacaa tgctttacca cgcaggtcgg
    24181 caacaatggc agcccaccct tcacctttga tggtatcaag ggttttcagc gcggcatcag
    24241 cctgcttgag ctgtacctga acgcgagcaa gacgcagatt tatcacggct ttgagatttt
    24301 catcactcgt gtctgccagc ccctgttgta actgggcggc agctttctct agttcatttt
    24361 tgtcaacaaa ttgctgcgca agttccaaag aagccagcgc accataagta tttttatttt
    24421 cagcagcaaa tttttccgcc gccgggatgc tatccggttt gccttcgctc actgcggtaa
    24481 ccgcattttg ataggcaaga gaagcggagc gtgcagaatc aacctgatgg ctgttccagt
    24541 agcgccagcc aatcagtgcg ccaacgccca aaatcacccc aacagccagt gctttgccat
    24601 tttcagcaaa aaagcgttta accgcttcta cctggtcgtt ttcgttctcg taaatttcca
    24661 cgctgtcctt ctccttcctt aacccagtaa cgtgcgcaaa tgcgcggcta cgctatcctg
    24721 cgcaactgcc gtttgctcac cagagcgcaa atccttcact actgctgtgc cgttagccac
    24781 ttcagactca cccagcacca cagcaacgcg ggcaccccat ttatcagcac gggcaaactg
    24841 tttcttaaag ttgccgccgc cgtggttggt catcaatttc acgcccggta attcatcacg
    24901 cagacgctca gctaatgcca tagccgcaga ttgtgtatca gcacctgaag ccaccaggta
    24961 tatatcgaca acaggatcgg ctttaaattc cggattaacg gcctgtacta acaatacaag
    25021 acgttcgagg cccatcgcaa aaccgactgc cggtgttgca cgaccgccca gttgttccac
    25081 cagaccgtca taacgaccgc ctgcacacac ggtgccctgg gagccgaggc tgttagtcac
    25141 ccactcgaaa acggtacggt tatagtaatc cagaccacgc accagacgct ggtttacggt
    25201 gtaagcgatc cccgcgctct caagcagttt gcacagaccg gcaaaatgct cacgagattc
    25261 ctcgtccaga taatcaccta atgccggagc gtcgttgaga agcgcctgca cttccggatt
    25321 ttttgaatcc agcacgcgca gcgggttagt gtacatgcgg cgtttgcagt cttcgtccag
    25381 cttttcttta tgctgctcaa ggaatgccac cagcgcatcg cggtaattgg cgcgtgcttc
    25441 cagcgaacca atagagttca gctcaagagt tacgtgttcg aagataccca gcgcacgcca
    25501 ccagcgggca gtgagcataa tcagttcagc gtcgatatcc ggaccttgca gaccgaaaac
    25561 ttcgcagccc aactgatgga actgacgata acgccctttc tgcggacgct cgtgacggaa
    25621 catcggcccg atataccaca gacgctgttc ctgattgtac agaagaccat gctcgatgcc
    25681 ggcgcgtaca cagcccgccg tcccttcagg gcgcagagtc aggctgtcgc cattgcgatc
    25741 ctcaaaggtg tacatctctt tttcaaccac gtcggtgact tcaccaatcg cacgtttgaa
    25801 tagcggggtc tgctctacaa tcggcaagcg gatttcactg taaccgtagc tgccgagcac
    25861 gtttttcagt gtgccttcaa tgcgctgcca gatggccgtt tcgccaggca ggtaatcgtt
    25921 catgccgcga atggcttgaa tgttttttgc cacgtttatt ctctttctga atataaaaat
    25981 gaaccctcaa cgcttccctc aatgtttcgg gagccatgcg ggttcaatca tacacgggaa
    26041 gcgaggcgct tcccatcacg ttattatttt tcaacctgct gaacgtcaat tcgacgcgct
    26101 tcgtccagct gactggcttt cgcacgaatg cgtgcttcca gctggtcgat catatcgttg
    26161 ttgtccagac ggtctttgcg cacgccatct tcatagaggc cgcttttctt gttgccgccg
    26221 gtgacgccga gtgtagaaac cagcgcctca cctgggccat tcaccacgca gccgataatc
    26281 gaaacgtcca tcggagtgat gatatcttcc aggcgttgct ccagcgcgtt aaccgtaccg
    26341 ataacatcaa attcctgacg cgaacaggtc gggcaggcga tgaagttaat ccctcgcgaa
    26401 cggatacgca gcgatttcaa aatatcgaaa ccgactttga tctcttcgac cggatcggcc
    26461 gccagcgata cgcgcagcgt gtcgccgatg ccttccgaca gcagcagacc taaaccaatg
    26521 gcggatttta ctgccccgct gcgtgcacca ccggcttcgg tgatccccag atgcagcggc
    26581 tgatcgatct gttttgccag caaacgataa gactcaacag cgaggaagac gtcagacgct
    26641 ttcacgctga ctttgaactg atcgaagttc aggcgatcga gatgatcaac atgacgcatg
    26701 gcggattcca gcaacgcctg cggcgtcggt tcgccatact tttcttgcag atctttttcc
    26761 agcgatccgg cgttaacgcc aatacggatc ggaatgtttt tatcgcgcgc acagtcaacc
    26821 accatgcgaa tacgctcttc attaccgata ttgccagggt taatacgcag acaatcgacg
    26881 ccgtattccg ctactttcag cgcaatgcga tagtcgaagt ggatgtcagc caccagcggc
    26941 acgttaaccc gctgtttgat gagtttgaac gcttctgccg cgtccatcgt cggtacggag
    27001 acacggacga tatcagcgcc aacgcgttcc agcgccttga tttgattgac cgttgcttcg
    27061 acatctgtcg tacgcgtatt ggtcatggac tgtacggcga tgggagcacc atcgccaatc
    27121 ggcacattcc caacgtaaat acgtgttgat tttctacgtt gaattggagc ctggttatgc
    27181 atgaaaaatc tcccgcgtta cccgtctgtt actgcgccgg tgattgttcg gcattgaggg
    27241 tcagacgcgc aacctggtta gttctgataa aacgactcag atcgacaggt ttcccttgat
    27301 actggatctg tactgcggct ggcgcaccaa ttttcagttt atacggtgcc tggccggtta
    27361 aattcaaatt accgtcttta cgctgcatcc cgctaaacaa ttttttaccg gtagcatcag
    27421 tgacctccag ccagcaatcg gcagtaaagt tcatcaccag cgcattcgga tcagccgccg
    27481 gcgtggtcac gccagcctga tcggttggca acggcgcagc accatctggc gttgttgctg
    27541 ccgttggtac cggggtcgcg gcggtatcaa catttgcctg cgaaggcgaa acaaccgcat
    27601 tctgttgcgg atcaacagct ggtgctggcg cagttacggc aggtgtttgc gtgttggttg
    27661 cggtagtatc cacagacgct ggcggcgtgg acgttgtagc cgggtctgta gttgtcgacg
    27721 tatttaacgg gacactctgc ccctgctcgc tattactgct cagttctgcc gaagattgat
    27781 cggccatagt ggtgatctct tcctgctgag ctttgtggtc ttgccaccac caggcaccgc
    27841 tcaggccgat aaccacaaac aacaccagcc aggtgaaggt catcagccag ccatcacgtt
    27901 ttttgcggcg tttaccgagg gaaaaactct gcattggcgc aacttttgca gcccgtagtg
    27961 gagcctgctt ttccagccct ggcagcagtt cttcttctgg aatatgtacc agacgcgcat
    28021 aagagcggat atatccgcgc aggaatgttg aagcaagatc ggcgggtgcc ttatcttctt
    28081 caatgtcgcg taccgtggaa accttcaggc aaagtcgctc ggcaacggcc tgctgactaa
    28141 gtcctagttg ttcgcgagca ttacgcaggc gagcgccggt agtaagtgct tcattttggt
    28201 cgtgcgtggc ttcagtattc attcgctgca ggtacgttta aatgagaatt gagatgccgg
    28261 tgaaccatca tgctcaccca caccgcgaaa catccgttaa gttaaccgtt atcatacagt
    28321 ataagaccgt ctgtgtgctc gtgacaaagc ccacattcac taaggctaac ttactgttgc
    28381 atcgttacat actgccttaa agtcagcaaa aacgcaccgt taatattgac cagacaattg
    28441 caacttaata cttcattcat aaaacattac gccacggtac ataaagtaac cgtggcgtaa
    28501 tggctatcag accgctttaa tgtcgatggc ttcaccctgc atccgtttac gcagggtacg
    28561 tttcgtacgg tcgataacat cgcccgccaa ctgaccacag gcagcatcga tatcatcacc
    28621 acgagtttta cgcacaatag tggtgaaacc gtagctcatc agcacttttg agaaacggtc
    28681 gatacggctg ttcgagctgc gtccatacgg cgcacccggg aacgggttcc acgggatcag
    28741 gttgatctta cacggcgtat ctttcagcag ttccgccagt tggtgcgcgt gttcagtgcc
    28801 gtcgttaacg tggtcaagca tcacgtattc aatagtgact cggccctgat tggcgttgga
    28861 tttctccaga taacggcgca ccgcagcaag gaacgtttcg atattgtact ttttgttgat
    28921 cggcacaatt tcgtcacgaa tttcgtcgtt cggcgcgtgc agggaaattg ccagtgcaac
    28981 gtcgatcata tcgcccagtt tatccagcgc cggaactaca ccggaagtag aaagcgtgac
    29041 gcgacgttta gacaggccaa aaccgaaatc atcaagcatg atttccatcg ccggaacgac
    29101 gttgttcagg ttgagcagcg gctcgcccat gcccatcatc actacgttag tgattggccg
    29161 ctgaccggtg acttttgctg cgccgacgat tttcgccgcc cgccacacct ggccgataat
    29221 ttccgacacc cgcaggttgc ggttaaagcc ctgctgggcg gtggaacaga atttacactc
    29281 cagcgcacac cccacctgcg aagagacgca gagcgtggca cggtcgtctt ccgggatata
    29341 caccgtttcg acgcgctgat cgccaacggc gatcgcccat ttaatggtgc cgtcggatga
    29401 acgctgttct tcaaccactt ccggtgcgcg gatctccgcc acctctttca gtttgccgcg
    29461 caacactttg ttgatgtcgg tcatctcatc aaagttgtcg cagcaatagt gatacatcca
    29521 cttcatcacc tgatcggcgc ggaagggttt ttcacctaaa tctttaaaaa actcccgcat
    29581 ctgctgacgg ttgagatcca gcaggttgat ttttccatct ttcgtggtga cgttttcagg
    29641 tgtgactaat tgttcagaca tatgctattc cggcctcgtt attacacgtt atggcccctg
    29701 gagggttgaa aaaagaaacg ccccggtgag cggctgctcg tccgggggcg ctgcattgta
    29761 caaattctgg cgcacggatg ccacgtttgc acgcggcatt tacgaaatta ttaacgggtg
    29821 cgcgggcaca cttcgccttc gccgaagaaa taagcgattt cgcgagcggc agattcgacg
    29881 gaatcagaac cgtgggtacc gttttcggtc aggctgtcag cgtaatcagc gcgcagagta
    29941 ccggccagtg cgtttgccgg attggtcgcg cccagcagat cgcggtgacg ctgaacggcg
    30001 ttttcacctt ccagcacgga aaccacgatc gggccagagg tcatgaattc aaccagacca
    30061 tcaaagaacg gttttccatc gtgttcagca taaaagccac gagcctgttc aacggtcagg
    30121 tgcagcattt tggtgccgac aattttgaac cctgcagctt caaagcgcgc aaagatatta
    30181 ccaatgacgt tttttgctac cgcgttcggt ttgatgatgg aaaaagtacg ttcaatagcc
    30241 atttttacct ctgtaaattg ttctgttgtt gtctgtacca gcgtacgaga tggcgcggat
    30301 tataatgagc aacagggccg ttgactattg atgaaggtaa cattttttta aaataaaatg
    30361 agttttagca acaatcaggg acaaaacaga ctattgcatg acaaatttca ctgtcgcgat
    30421 ttgccccacg tcatccatca ccagcaattg ataatcacct ttatccgtca aatgcagggt
    30481 cacgttgcgc ccgcgttcag ttaacggttc gccattcaga aaccaccagc gttcacctgc
    30541 ccctccactt gattgcaacg gcaaagtcgc ttccgctgcg cccggtaaac gtttaataat
    30601 cgcgccatcg cgaacgcctg tcagctgcag agggagctgg gcatcgtgac cgtatggcgg
    30661 gcaaattgtc gaggccggtg gtaagcgcac agcgcgacgt tctgatgcgg gcagccaggg
    30721 ttccagcggt agcggccaga catttaccat ttcttgcttc gcctgcgggc agtcagcggc
    30781 gacgcgctgc cctttgtcat ccagccagat ggggaagcga atgccattaa tcccttcctg
    30841 ctctgacagt aacagcgttg gcggctggct gccatccagc agccaggttg ccaggcggcg
    30901 gcgacagtta ccgtcacctt ccggcaaaga ctgtccgccc ggccagcaga taacgccacg
    30961 agtgactgag ttcgggcgcg ggtcttccgg cagattcgca ctgcgcgaca gtaagatatt
    31021 attgacctga ttcaacaatg gtacggcact ggcaaagcca aactgaccaa caacgggcgt
    31081 gccgtcaggt ctgccagtcc agatcccaat gacatagcga gcgttaaccc caatcgccca
    31141 ggcgtcacga tagccatagc tggtgcccgt tttccatgcc agtggggcga cgcgcggcaa
    31201 ggcactatcc ggcaagggtt gcgcttcatc agccataatc cggcgaatga tccacgccgc
    31261 ccccgacgac attaaaggcc gttcaagcag cggatcgtca ggctgtaagc gcaatttgcc
    31321 tgccttgccg tggcgagcaa acgcggtata cgctgccgcc atatcttcca gttttgcacc
    31381 agcgccgccg agaatgagtg aaagattcgg cgcagcgccg ttgggcaaat ataacggcaa
    31441 tccaacattg cgtaactttg ccgcaaaccg tttcggtcca taggcttcca gcacctgcac
    31501 agcaggtaag ttcagcgagc gcaccagcgc ctcgctcatg ctgatcgggc catgaaaacc
    31561 gctatcaaag ttacctggtc gataatcacc ggtgcgccgg gggacgtctt gcagcagtga
    31621 tgccgggtgg atcaagcctt catccagcgc cagaccataa acaaacggtt tgagcaccga
    31681 tcctggcgat cggatcgcat tgaccatatc aacatgacca aagcgtgaat catcgttgag
    31741 atcaaccgat cccacccagc cgcgaacacg catatcggta tgatcaacca cgatcatcgc
    31801 cagtgagctg cgcggtggca accgcccttt ccagttttgc gccagttctt ccagacgtcg
    31861 ttgaagaccg gcatccagcg tagtaacgat tttgtcgctt ttgcttttac cgagcatcat
    31921 gcgcgaaaac agcggtgcca gttgcggcat ttgtcggggt gccagccaga tgggttcttc
    31981 ccttgactct tttacctgct cacgggacca cacaccttgc gcagccatcc gttcgagcac
    32041 tttattacgc gcggcttcgg cacgctccgg ccaacgatcc gggcgaagac ggctgggcgc
    32101 ttgcggcaaa accgccagca ttgccgcctc ggaatagctt aaattcgcag gcgactttcc
    32161 gagataagcc caacttgccg caccgatccc ctgcaacgta ccgccaaacg gagcgcggtt
    32221 aagatacaag gtcagaattt cacgcttaga cagatgccat tccagttgca acgcgcgcca
    32281 gagctggcga attttgccgc caaatgtttt ggggtgagga tcaagcagac gagcaacctg
    32341 catagtgagc gtgctgccac cggaaataac ccgtcccgaa gtgagatctt gccatgctgc
    32401 gcgcgccacc gagaatggat tcacccccgg atgcttccag aaccagcgat cttcataatt
    32461 gatcagcgct tcaaggtaac gtggagaaac atcttcgatt gttaccggat aacgccagat
    32521 gccgtcagca tcggcgaagc gccagagcgg cgtaccatcc tgcgccacga ccactcgtgc
    32581 gggattgact tcatgcagcg gtagaggcca gagtttatct gctccccacg ctgcaagaaa
    32641 aaggagaaat ggcgcagctg ccagcgttat ccagcagccg cgtttggtta acaagcgagg
    32701 catttacggt cggacaatca gcagatcttc agccgcgccg gtcgcccgcc attggggaac
    32761 atacattgat tccaccatcg gttgcggtac ctgatacgtt cccggcgtca ccgcccgcgc
    32821 cagatacacc agcgttaccg gttggtattc atcaacggca accgccgcca caaagcgatc
    32881 gtcacggaac tcaatgtgct taatactcgc ctgctgcatc tggttcagta agttctgcac
    32941 ttcgccacca ctttgctcca ggctggcgct accgttcgcc agattctggt tttccagttc
    33001 cagacccaca ggcagcagat ccacgactaa cgcatccggc acgctgttac tggcttttac
    33061 ctgcaaccac accagcacca gatcgccgct acgtaacgag tccagcgatt tgctcttacc
    33121 atcagtacca agaatatgac gctcgatttg cagcacattg ttcgccggta aaggtgcgga
    33181 ttgcggataa ccgctggcat ccatacgcaa ccataacggc tgatcgccac tgttgctcac
    33241 ctgcaaggtg acaagttgat cgctattcag attgctgttt tgcgctttct cgcctgtcag
    33301 cggctcagct gagaaagagg tttgcgcctg ccatttaccc ggtaaatcct gaatcgtacg
    33361 ggcagccagg aacaacgcgt tactttcctg cgtcgatagc cagcgttcac caaacgcctg
    33421 ctgcgaaagt gtgttcagca aagtgtactg ctcatcgggt agcagtttat tttcttccag
    33481 caaggagagc attaacgcgt tgtcgcgcag tggactaccg taatcaccca gccatatccg
    33541 ctcatcacta tttctcggcg ttttcagcgc cagcgcaatc gcttcttcac cacgcgtcgc
    33601 atcacccatg gttttcagcg caatgccaag ttgcagcagc ggtaaaccag aagcggcatc
    33661 tgcacgatgc tcccagattt cacgcagcgc acccagtggt gctttttgct gacgggccag
    33721 caccagcgca gcgtaagact gaacggcgaa tttactggct ttgagattat ccgcgtacgg
    33781 gatcgacatc atgcccggat cttgtaaata gcgcagcaga cgctcattac cccggttaat
    33841 ggcgtctgtc ggcacgctgt aaccctgttc gcctgcgcgg accaggaaat ccatcacgta
    33901 agccgtcagc cagtactctt cgtcaccgtt tttatcccac agcgcaaagc cgccgttatc
    33961 acgttgcatt tgcagcaaac gggaaatgcc gatatcgacc gatgcacggc gtttctcatc
    34021 actgtcgcct ttgatgccca acgcctgcag ttgggctgcg ttggtataaa gtgacgggaa
    34081 caggccgctg gcggtttgct caagacagcc gtacggatac gcttttaact ctttgatata
    34141 acgtgcaatg ttcagcggtg gtttgccgct caacaacaat tgcccttcca gcgtaacagg
    34201 cgagaagttt tgcaatccat ccgccggaat cgcccatgtc tcaccaggct gtaacgccgt
    34261 accgtaatta accgtttgtg ccgggaacgc cggacggacg ccgattttcc actgcttatg
    34321 ctgatcggca acggtttcac ccggtaacgc taacccgcta atggtggcct gaatttctcc
    34381 atcgccataa cccggcaatg ctcgcaccgg gataaacagc gtagtacgca cacctggcgc
    34441 taattcaacg gctgcgggtg aatcgctgac cagttcaagc aaaccactgg cggtcagggc
    34501 aacgttcagt ttttgcggtt tatcggtaag attagtgata tccagcgtca gacgcgaggt
    34561 atcgccactc gccataaagc gcggcatgtt cagttcagca atcaccggtg cggcaactat
    34621 cactttgctt tcgttgctac cgaaatcatc tgccgtccag gcttgcgcca tgacgcgcaa
    34681 ttcaccgtta aaatcgccaa tcggcagtgt aaccgagcct tcgccctgtt cgttgagcgt
    34741 taccgacagc gcctgctgcg cgacaatatt gacgtgattg accggcggtt taccaccacg
    34801 tttcagctca tcaccatcgc caccgaaacg cagagctgcc agacgcccct gaccttcaat
    34861 aacctgaccg taaatatcgt aaatgtctgc gccatagcgt ttctgaccaa agaacgcctg
    34921 ccacggatct ggcgtaacgt agtcagtaat attcagcaca ccactatcaa cggcagacac
    34981 cagcacattc acctgtttag gcttctcgcc atttttagtg ctggctttaa ttttcacggt
    35041 taatggttga ttgggacgca tttttgctgg tgtttccagc gccagatcga ggcgacggtt
    35101 ttcatcacca agcggcagat gcaacacacc aaccgcgcgt tttggcgtcg cggagcgaga
    35161 tttatcgcca ggacgcacca ccagcgtact caaataaaga tcatgacgat tccaggtttt
    35221 atcgaccgga atcgtcagat ccagcccttg agccggaaca tcaatctctt gccaccacag
    35281 cggcccttca ctggactcga ccatcgcata acctttaccc gccgttggcg cggcaatatg
    35341 caacttaatg gtatcgccgg ggcgataact ggctttatct agtttcagcg tgacacggtc
    35401 gggtcgcact gcgccgctcc cgtcgctgtt gtcctgccag ctatagccag cccaaaaacg
    35461 cacgctactg accgcttcat tcggcgcttt gacttccaga cgataagcgc cccactctac
    35521 cgggaaactg accttaccgg tttcatccgc ctgaagatcc agagtttgtt cattttcgat
    35581 cagatctttt tgatcaaact gtgactgcca gccttcatct tctgaccagt tccagtagta
    35641 atcgcggcgt tcgcgaatca ggcgcacctg caagcccgac acggcttttt tcacgccttg
    35701 cgcatcgcta taaacgatgt caaaagcggc gttactgcct tcatcaacaa tgggctgttt
    35761 caccgtgctg tcagtgcgat aatcgtaaac cgatttcaag gcgaactgcg gacggagccc
    35821 cggcaatgca tcggcaggcc agatagcctg ctcagcgcgg cgcgtcaccg ggcgaccgcc
    35881 cgattccagc aaactgccct ggaaaataac ctgcaatggg gaatgcgttt ccttccactg
    35941 gctttctgta gaaacttcgc cgcgcccttt atcatccagc gtcaactgaa cttcatccag
    36001 cgtgcgggaa aggttttcgg cagctatatc gccgaattca aaaccaggta aggctgacac
    36061 agcctcacgc agtgggcgca ggaaaagttg cccttgcaaa gtattaccat tagcaggcgc
    36121 accgtacagg tagtatccca ccacggagaa tttcacttca tcattcggcg taagcggggt
    36181 tttctcaccg gtcagattca gcgccatacg ctctggcata aaatcttcga cgtggaaatc
    36241 ccacatccgg tactggttat cgcccgtgtt ggcgcgaata tgccacatac cggttgccgc
    36301 attgctatcg agtggccagg taaagtggta gaggccattc tccggctgac taacgacgtt
    36361 cctgagtacc tgcccgtcag gtttaatcac gtctaacttg atgggttgat cgggcaatgc
    36421 tttaccgtct gcatcgcgca gtaaaccatt gaggattacc gtttcacctg ggcgataaag
    36481 atcgcgtggg ccaaacatga aaaactgttt gctatagcct ggcgcgccag caatgttaaa
    36541 ttctgctaag tccagcgccg gaagttttaa atcgagtagc gttgtctgac cgtctttacg
    36601 tgccagcagt aatgccgcat ttttatcatt ttccagctgc acatgcccct gagcgtcact
    36661 ggttgcctga gtcagagtct gtcctttctc atttaataaa gagacttcaa ttccttgctg
    36721 ggccgcgccg ttttccagac tttgggtaaa gatatccaga cgattgtgat aacggtgagc
    36781 tgaaacgccg atatcactta acgtaaacag cgtcgcggga ttactgtaat cgtaacgtcc
    36841 agcctgattc atcacagcca gatacacgcc cgcctgctga agcggtttga tatcgcccag
    36901 cggcagcaat aatttttcac gggtgttacg cgcaggattg agatcaaacc gtccggtgta
    36961 gaccagatcc gccatctgca gcagtttgtc tgactgccag ttcgccagcg aattgcggta
    37021 ttcccattgg ctaatgaatg ctggcagaga ttctggctta acgcggaaga agttaacatc
    37081 gacattatta acgttgagcg ccattaccgg cagcccttca acgactttgc caggcagcag
    37141 cgaaccacgg ctggcaaaac cgacgctggg ttggatgtcg cgggtagtta tagttttttc
    37201 gtaatcttta ctgaaggttg cgttgttgag tgctttgacc tccttgccaa tagtaacgat
    37261 caaatcacgt ttcggttcga ggtggcgtaa acgtagctct ttaagattat ctgacagctc
    37321 ccaggcacca tccactttgc cgcttttttt atcgacgaca tgaataacgc gtgagaaatc
    37381 ctgatccggg tcgagaggga tggagaacgt cagcaccagc gtggctgcac catcaagttg
    37441 gacttcagag agatccagca atgtcagcac cttaccggca ctctgttggg ccagtttttg
    37501 tctctccggc acggagagtt ttgctgaact cgcgttttct gaagaggccg ctttagtaac
    37561 ttcagaaggc gcatcttttt tcaccgctgt tggcgcgtta tcgttgttgt cgcaccctgc
    37621 cagcgccagc attagcatgc aggcggctac gcgtaacttt ttcatttttc atccctgcca
    37681 caatggcccg ttagcaacgt cgaataatta ttatgcgtga gaatttacgt tatgtaattc
    37741 agtatcacgc ccatacaatc cgaattttag tgagaattgt ggctcagaca gccaaatcag
    37801 cctctttcgc ttgtcgcttt tcactaaaca tccgacaatt tagcctaacc ccggcaaaaa
    37861 tggagatgcc tatgtccacg acatggtttg taggagccga ctggctcgcc gaacatattg
    37921 atgacccgga aattcagatt atcgatgccc gcatggcgtc gcctggacag gaagatcgta
    37981 acgttgctca ggagtatctg aatggacata ttcccggcgc agtgtttttt gatatcgaag
    38041 cgctttctga tcacacttcc ccgcttccgc acatgctgcc gcgcccggaa acgttcgccg
    38101 tggcgatgcg tgaattaggc gttaaccagg ataagcacct gattgtctat gacgaaggta
    38161 atcttttctc agccccacga gcatggtgga tgctgcgcac ctttggtgta gagaaagtgt
    38221 cgattctggg gggtggactt gcaggctggc agcgcgatga tctgctgtta gaagaaggtg
    38281 cagtagagct gccggaagga gagtttaacg ccgcgtttaa tcctgaagcc gtggtgaaag
    38341 taaccgatgt attattggca agccatgaaa atacggcgca aattattgat gcccgcccgg
    38401 ctgcacgttt taacgcagaa gttgatgaac ctcgcccagg tttacgtcgc ggacatattc
    38461 ccggagcact gaatgttccg tggacggaac tggtgcgcga aggcgaacta aaaacgaccg
    38521 atgaactgga tgcgatattt tttggtcgcg gcgtcagcta cgacaaacca attatcgtca
    38581 gctgcggctc tggtgtaacg gcagccgtgg ttttgttagc actcgcgacg ctggatgtgc
    38641 caaacgtgaa actgtacgac ggcgcatgga gtgaatgggg cgcgcgggca gatttaccgg
    38701 ttgagccagt gaaataagta ttttacaggc aataaaaaac cgccgaattt ggcggttttt
    38761 tattgctggt ctggttcgcg gcctttccag caggttgtat taccgtagta atgcaagcgc
    38821 gtctcagcgg agacaatact cgccagtaac tctctttttg tcaagcaaaa gagagttatt
    38881 attgttctgt tagtgtatta tccactgcgg ccctttccgc cgtctcgcaa gcgggcgctg
    38941 gcttaaggaa aggatgttcc gcgaccgtta gagcaagcgc gtctcagcgc ttgctatcgc
    39001 ggcaatatcg ccagtggtgc tgtcgtgatg cggtcttcgc atggaccgca caatgaagat
    39061 acggtgcttc tgtatcgtac ttattgtttc tggtgcgctg ttttcccaag taaataataa
    39121 ccggagtctc tccggcgaca atttactggt ggttaacaac cttcagagca gcaagtaagc
    39181 ccgaatgccg ccctttaggg cggcatattt tagattatcc ggttttgttt aaagtcacgc
    39241 aaaaaaccac cccagcgacg ttcatagaat ggcacaatat gttcggtaat aaagtggcta
    39301 attccttttt cccctttttt cacctgacaa atatcgattg gttcatcgcc aggtaatgta
    39361 tcggtcgcta cacttcccgt cgcctgaata atttcttcga tatcaccatc ggcttcaatg
    39421 ccaataagta aattaggctg tgcctcttcg ttctctttaa ttgaacaaat aaaagcacgc
    39481 ttcaccggct taatggtttt aaataaggtg gtgagtgaat caatcatttg tgctggcggc
    39541 tctgcgactt ccgataatat cagcgattca ccgccttcca ggatttcctg gctgctcagc
    39601 ggatttccct cttcaccaat caacaaactg atttcacgcg gcataaattc tttaccggtc
    39661 ggcagtttgg cattaaggaa gagcgtttcg ccaagggtca tctcaaacaa tgtgcgaacg
    39721 ggcattacga caaatgcctg ttcgtcttca accgcctgtt gaagcgcttc taacgaggtg
    39781 aaaaaaggaa tgacgctggt gccgtcttct ttttcccagt gctgtaaatc aagcgcacta
    39841 tcttcaacca cagcctcgcc ctgcgccgcc gtaccaggca cccagacggc ggattccagt
    39901 agagtacgga aaaaggccgg gcggtgcgcc ggttcggttg ctgctttttc cagcaggtct
    39961 tcaagttcgt tttttgtttc ggacataaga accacaattc attcaacgtt cggggcgcaa
    40021 atgcctgatg cgctacgctt atcaggccta caagggattc gcaatttgtt gaatttgcag
    40081 aatttgtagg ccggataagg cgtttacgcc gcatccggca tattagttta cgccgttaac
    40141 agattagcta tcgtgcgcac accaagtccc gtagcgcccg cagaccactg ttcaaccggc
    40201 gctttacggt aagtcgccga gcagtcgata tgcagccagc cttgctgata gttctcaaca
    40261 aagtgcgaca ggaagcccgc cgccgtgctc gcgcctgccg gatacgccgc gcttccggta
    40321 ttgttcagtt cggcaaagtt agacggcagc tggctgcggt ggaattccgc cagcggcaga
    40381 cgccagaatg gttcgttctc ttgtgaggca ctcgccagca aacgaccggc aagcgcatcg
    40441 tcaaaactga acagcgcgtg ataatcatta cccagcgcag ttttcgccgc cccggtgagg
    40501 gtcgccgcat caatgatcat ttccggtttc tgcgcactgg catcaatcag accatcggca
    40561 agcaccagac gcccttcggc atcagtgttc atcacttcaa cttttttacc gttgcgatag
    40621 gtgatgatat cgcccagctt gaacgcattg ccgctaatca ggttatccgc acagcagagg
    40681 aacagcttca cgcgcttgtt cagtccgcgc gtaatggcaa atgccagcgc cccggtaacc
    40741 gttgccgcgc cgcccatgtc cgacttcatc gagtccataa acgcagtctg tttgatgctg
    40801 tagccgccgg agtcaaaagt gataccttta cctaccaggc acgcgtacac tggcgcttct
    40861 ttatcgccag ttgggttgta atccagcgcc agcaataccg gagaacgttc tgaaccgcgt
    40921 ccgacggtgt gcagccccat ataaccttgc tcacgcagat cttcgccttt ggtgatccga
    40981 taagtcacac gatcgcccgc gacgttgctg atcagatcaa cagcacgctg tgccagttgc
    41041 gatggtccca attcttctgc cggtgcgttg atggtgtcac gcacccagtc gatgatcatc
    41101 aggcggttat ccagttcctg gcgctgggca tcgtccagat ccggccactc cactttacgc
    41161 gtgcctttcg gggctttgta accttgccag aatgcccagc agcgatccgc atcccagcct
    41221 tcaccgctta actgaacatg cttgatgccc agaccgtcaa tcttgcgcgc cgcacgctgg
    41281 atcagcccca gatcgtctgc cccgttcaaa tgcagggtaa tgccgtcatt attaatgctg
    41341 taagttgctt tttctcccca gcgcgcgtcg gcaggttggg tagagagggt aatcttcatc
    41401 gcttctgtca ttttagttat ccttctttgt aaaagggccg ccagttggca gccccaggat
    41461 ttttccgcta aatgattcgc gttgcagaaa ggcggcaagt gagtgaagcc ccaggagcat
    41521 agataactat gtgactgggg tgaacgagcg cagccaacgc atctgcggcg tgaagcatga
    41581 cgcggaaatt attcggcctc gtccagccag actaacaaaa tcgcttcgag aattttctcg
    41641 ttggatgcct gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg atgcatatcg
    41701 gtgaatcgaa ccgttttcgg atcaagatca ggatacgcat cgtacagtgc ttcgccaatt
    41761 tcgcggctat cggtccactt aagtcccata ctaacctctg ttaatgctca cgcgcatggt
    41821 tgatagtgta acgcgggatt tcgactacta aatcttcgtc ggtaacacgt gcctggcagc
    41881 ttaaacggct ttcgggctcc agtccccagg ctttgtccag catgtcgtct tcctgctctg
    41941 agctttctgg cagcgagtca aaaccttcac gaacgatgca gtggcaggtg gtgcaagcac
    42001 aggatttttc acaggcgtgt tcaatctcga taccgttacg cagagctgcg tcgagaatgg
    42061 tttcaccgct attagcttcc agaacagcgc catcaggaca gagatcctga tgaggcaaaa
    42121 taacaatctt tggcatatta aacctcgtcc acggaatggc ctttcagcgc acgacgaacc
    42181 gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt tttaatcgct
    42241 tgttcgatgg cgtcaacgtc atcgccctgc gccacttcac tcaggtgagc ggcagcatcg
    42301 tcaatgacct gacgttctgc ggcgcttaac agcgcggcat cagcagccag cgcgccgtgc
    42361 agactttcca gcacacgcgc cgcttctact ttttgttctg ccagcattcg ggcttttacg
    42421 tcctgctcgg catagctcat tgagtctttg atcatcgaag cgatttcgct atcggtcaga
    42481 ccgtaagacg gtttgacctg aatagacgcc tcaacgccgg tggatttctc catcgccgtc
    42541 acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc accaccagcc
    42601 ggtagcgccg gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc ctgcaccagt
    42661 tcgcgctcac cctgcattac atggatagac atcgccgtct gaccatcttt aaaggtggtg
    42721 aaatcctgag cgcgggccac cggaatagtg gtattacgcg gaatcacttt ctccaccagg
    42781 ccgcccatcg tttcgaggcc cagcgacagt gggatcacat caagcagcag catttcgctg
    42841 tctggcttgt tacccaccag aatatccgcc tgaatcgccg cgccaatagc gacgacttta
    42901 tccgggtcga tggaagtcag cggtggacga ccgaaaaatt cgcctacccg ttcacgcacc
    42961 agcggcacgc gagtagaacc gcccaccatc accacttcca gcacttcgtc agcttctaca
    43021 cccgcgtctt taagcgcgcg acgacaagcc agtaaggttc gttttaccag tggcgcgatc
    43081 agttcattga attgttcacg gctgatttcg ccctgccagc ccgcaacgtt aacggtcacg
    43141 gagtccgcat cgctcagcgc gattttggct gcaatggcgg catccagcag ttcacgctga
    43201 acgcggttat cgctacgatc aggaatgccc gcctgctcgc gaatgtaatc cgccagcaga
    43261 tggtcgaaat catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac ttcaaacacg
    43321 ccgcgactta agcgcagaat ggaaatatca aacgtcccgc caccgaggtc ataaacagcg
    43381 atcacgcctt cctgaccgga atccagcccg taggcgatag ccgcagcggt cggttcgtta
    43441 agtaagcgca gaacatgcaa ccccgccaga cgcgccgcgt ctttggtgcc ctgacgctgg
    43501 gcatcgtcaa agtacgccgg aacggtgata actacaccat ccagctcgcc tgccagggct
    43561 tcagttgccc gcgccgccag tgctttgagg atgtccgcag aaacgcgcac cgggttcagc
    43621 agccccgccg ccgtttcaat catcggcagg ccgttttcgc tggcctggaa ttgataaggc
    43681 agatgcggat agcgttgctg gatatcagcc agcgagcgcc ccatcaggcg tttaacagaa
    43741 ctaatggtgt tggcggtgtc aagcgctgcg ttagtacgcg cgtcataacc caccgaatgc
    43801 ccttgctgtt gatagtgaac aacagatggc agcaggtgac ggccttcatg gtcggctaac
    43861 gtttcggcct gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc caggtcaata
    43921 ccagccgcca gacgacgctg atgcggcgca gcactcaaac caggttcact aatttgtaat
    43981 aaggccatgt ttagcttcca gaaattaaaa atcgagcagt ttttcttcga gttgttcggc
    44041 actgctccgc agtttatcga gaaaacgcag cttacgcacg gtatccgccg ccgcgtccca
    44101 cgcctcgttg tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca tctttttcac
    44161 acgtttgata aagctttcca gccgcgcttc atctttcgcc tgttcgatct cgtccagctc
    44221 ttcgcgcagc tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat gctgctcgct
    44281 ggcgagatca aaaccgtgta aagaaagcaa atattccgcg cgcattaacg gatgacgcag
    44341 cgtttgccag gcctggttaa tggttgcaga ttgctgtacg gcggcgagtt gttccgcctg
    44401 gcttccgctg gcgaatttat caggatgata ctgacgttgt agatcctgaa aacgcaggct
    44461 cagcgcctgg gtatcgagtt gataacgggc aggcaagcca aagaaggtga agtaatccat
    44521 aacattctca gggctgcgat ccgccgcgat aacggcgaat caggtaaaac aaaccccacg
    44581 cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct ttcgccgcaa
    44641 ccacactcat ctttgacgtt cgggttggtg aatttaaacc cttcgttcag gccttctttt
    44701 acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac cacgactttc
    44761 acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa ttccagtaca
    44821 taagccatac ctgaacatcc ggaggttctc acgcccagac gcaggccaaa ccctttgccg
    44881 cggttagcca gaaaggtatt tactcgcgct gctgcactgt cgctcagtgt aatcgacata
    44941 accaaacctc aactcttatt ttgcttcacg tttgctttta tagtccgcaa tggcggcttt
    45001 gatcgcgtct tctgccagaa tagaacagtg aattttcacc ggcggcagtt caagttcttc
    45061 agcaatatcg gtatttttga tcgcctgcgc ttcgtcgaga gacttccctt tcacccattc
    45121 ggtgaccagg gagctggaag cgatagcaga accgcagccg taagttttaa aacgcgcgtc
    45181 ttcaatgata ccttcatcgt tgactttaat ctgcaacttc atcacgtcgc cacaggccgg
    45241 tgcccccacc atgccgctgc cgacgttctc gtcgttgttg tcaaaggaac ccacgttacg
    45301 cggattctcg taatggtcga taactttttc gctgtaagcc attataaatt ctcctgattc
    45361 cgataccgat taatgatgag cccattcgat gctgttcaga tccacgccct gcttgtacat
    45421 ttcccacagc ggagaaaggt cacgcagacg accgatggat ttacgaacta actcgatggt
    45481 gtagtcgatc tcttcttcag tagtaaaacg acctaaagag aaacggatag agctatgtgc
    45541 cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc ttgctgacgt
    45601 acaggcggaa cctgaagaaa ctgcgaggtc tttcagcgcc ataatcagcg actcaccttc
    45661 aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt caccgttcag
    45721 gtaaacttct tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc cgcgcagacg
    45781 ttccatctcg gtcgccatct cttcttttgc gatgcgatag gcctcgccca ttccgacgat
    45841 ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc cgtgcatttg
    45901 cgcttcgatg cgtacgcgcg gtttacgacg tacatacagc gcaccgatac ctttcgggcc
    45961 atagattttg tgaccggaga aagacatcag gtcaactttc aactggctca ggtcgatagg
    46021 cagtttgccc acgctctggg ttgcatcaac gtgatagata atgccacgag cacggcacat
    46081 ttcgccgata gccgcgatat cctgcaccac gccgatttcg ttatttacgt gcatgatgga
    46141 aacaaggatg gtgtcatcac gcatcgctgc ttcaagttct ttcagatcga taatgccgtt
    46201 acgctgcggt gccaggtagg tgacttcaaa accttcgcgc tccagctgac ggcaggtatc
    46261 cagtaccgct ttgtgttcgg ttttgctggt gatgatgtgc ttgccttttt tctgataaaa
    46321 gttggctgca cctttgatcg ccaggttgtc agattcggtt gcaccagagg taaagacgat
    46381 ttcacgcgga tcagcgccga ccagatcggc aatctgatta cgggcgatat ctaccgcttc
    46441 ttcagcctgc cagccgaaac ggtgagaacg ggaggccggg ttaccaaagg ttccgtccat
    46501 cgtcataaac tgcatcattt tctcggcaac acgcgggtcc accggcgtgg ttgcggagta
    46561 gtcgagataa atcggtaatt tcattgctct ataaactccg tacatcactc aatgcaagga
    46621 accaggctac cggctggatg tacgaccgtg tttacggagc gcgatacgcc ccggcctgat
    46681 tctaatatct tgttatcttt aacctaactg tttcgggttt cagcaagttg acaaacttac
    46741 gaatctccgg gagcttacat aagtaagtga ccggagtgag tatgtgcagt caacgcagat
    46801 gcaacacgaa acaggacggt taaattaagc gcgtaactta acgtcgatcg cgtcttgtat
    46861 gcgggtgcgt ggcgcgtcgt gagtatgctg acgaccagac acatccagca cttcctggtt
    46921 attaaccagt tcgcctaaag taatgttgtt gagaaaaccg gtgagacggt cgctcaaatc
    46981 acgccacagc gcgtgggtca ggcatttatc gccgccctgg caaccgcctt taccctgaca
    47041 acgggtagca tctacagatt cgtcaacggc gctaatcact tcgccaacgg cgatgctgct
    47101 ggcatctttg cctaacagat aaccaccgcc tggtccacgt acgctggaaa ccagaccatt
    47161 tttacgcaga cgggaaaaca gttgttccag ataagaaagg gaaattccct gacgttcgga
    47221 aatatcagcc aacggtaccg ggcccgcttc agagttgagc gcaacgtcaa gcattgcggt
    47281 cacggcatag cgccctttag atgtcagtct catgtcttac ttcacctcaa actcgcccct
    47341 gcccggggtt ttttattgta aaagtggggg tattgcatag cagggtcaag tctgacattc
    47401 ccgagtaaat tggtcaacta tttacttgac tgatttagtc gggtatttaa ccttcagtgc
    47461 cattttttta tcgtggcgtt tgtacctgtt gtccttccag accatgggcg ggttgagggc
    47521 aattattcgc attagcgctg gcctcttccc ctcaccctaa ccctctcccc gaaggggcga
    47581 ggggaccgtc cgtgcattta tctggcttag ccctcaggcc ttattacctt tattctgctg
    47641 ctctatagaa gccagaatcc cgcgcaggat attcaattcc tggctttccg ggcgcgcacg
    47701 ggtaaacaga cggcgcaatt tattcatcac ctgccccgga tggttttcac ggataaaacc
    47761 ggttgccagc agcgtttgtt ccagatgacc ataaaaacgc tccagatcat cgaccagcgg
    47821 atacggcgtc tcttcatgct cgacctgctc gccattttcc tgagttgcca gccaggccat
    47881 acgcacttca taggcaataa cctgaaccgc cattgccaag ttcagcgaac tgtattccgg
    47941 gtttgccgca atcgcgacat gataatggca tttctgcaac tcttcattgg tcaagccgac
    48001 gcgctcacga ccaaacacca gcgccaccgg ggtatttgcc gcttcagcga cgcttttcag
    48061 accgcattcg cgcgggtcga gcatcggcca cggcagcgtg cgggagcgtg cgctggtgcc
    48121 caccaccagg ctacaaccgg ctaacgcttc gtcgagcgta tcgacgatgt gagcattacc
    48181 aatcacatcg ctggcccctg ctgccagggc aatcgcctgg gagtcgggtt tcaccagtgg
    48241 attaaccagc cacagattgg ttaatcccat tgttttcatg gcacgggcaa cagaacccat
    48301 attgccggtg tgtgacgtct ccaccagcac aattcgaata ttttgcagca ttgtctttct
    48361 tcatctaaag attattcacg catcttatca taaaacgaag acagatgccg atctcgctgc
    48421 tatactctgc gccgttttcc cgttctttaa catccagtga gagagaccga tgcatccgat
    48481 gctgaacatc gccgtgcgcg cagcgcgcaa ggcgggtaat ttaattgcca aaaactatga
    48541 aaccccggac gctgtagaag cgagccagaa aggcagtaac gatttcgtga ccaacgtaga
    48601 taaagctgcc gaagcggtga ttatcgacac gattcgtaaa tcttactcac agcacaccat
    48661 catcaccgaa gaaagcggtg aacttgaagg tactgatcag gatgttcaat gggttatcga
    48721 tccactggat ggcactacca actttatcaa acgtctgccg cacttcgcgg tatctatcgc
    48781 cgttcgtatc aaaggccgca ccgaagttgc tgtggtatac gatcctatgc gtaacgaact
    48841 gttcaccgcc actcgcggtc agggcgcaca gctgaacggc taccgtctgc gcggtagcac
    48901 cgctcgcgat ctcgacggta ccattctggc gaccggcttc ccgttcaaag caaaacagta
    48961 cgccactacc tacatcaaca tcgtcggcaa actgttcaac gaatgtgcag acttccgtcg
    49021 taccggttct gcggcgctgg atctggctta cgtcgctgcg ggtcgtgttg acggtttctt
    49081 tgaaatcggt ctgcgtccgt gggatttcgc ggcaggcgag ctgctggttc gtgaagcagg
    49141 cggcatcgtc agcgacttca ccggtggtca taactacatg ctgaccggta acatcgttgc
    49201 tggtaacccg cgcgttgtga aagccatgct ggcgaacatg cgtgacgagt taagcgacgc
    49261 tctgaagcgt taatgactca ggcgggtgat gtcactcacc cgccctcgcc tttcaggcgc
    49321 tattctgaaa tacttcctca ccgctttact ttctttcgtc actctcccac catctttccc
    49381 cgattaatgg atgataaaga agtcacaagg aaaggttatg gcgctgccag tgaacaaacg
    49441 cgttctcaaa attctgttta ttctctttgt tgttgccttc tgcgtttatt tagtgccgcg
    49501 cgttgccatc aacttcttct attatcctga cgataaaatt tacggtcccg acccctggtc
    49561 ggcggaatcc gtcgaattta cggctaagga cggtactcgt ctgcaaggct ggtttattcc
    49621 ttcttcgacg ggccctgctg acaatgccat cgcaaccatc attcatgctc acggcaatgc
    49681 cggaaatatg tccgcccact ggccgctggt cagttggtta cccgagcgta atttcaacgt
    49741 ttttatgttt gattatcgcg ggtttggtaa atcaaaaggc acgccgtccc aggccggatt
    49801 gctggacgat acgcaaagtg ccatcaatgt ggtgcgccat cgcagtgatg taaacccaca
    49861 acggctggtg ctgttcgggc agagtattgg cggggcgaat attcttgctg ttattggtca
    49921 ggatgatcgt gaaggcatac gtgcggtgat cctcgactcc acatttgcct cttatgcaac
    49981 catcgccaat cagatgatcc ccggcagtgg ctacttactt gatgagagtt acagcggcga
    50041 aaattatatc gccagcgtca gcccgatccc gcttttactc attcacggta aagctgatca
    50101 cgttatccca tggcagcaca gcgaaaagtt gtatagcctg gcaaaagaga caaaacggtt
    50161 gatcctaatc ccggatggcg aacacattga tgctttttcc gatcgtcacg gcgatgttta
    50221 tcgcgaacag atggtggact ttatccttag cgcgttgaat ccgcagaact aacccatgat
    50281 cgctagcacg ataatcattc acaaaaccac cttaagacat gctaatccac tggtcagaac
    50341 agtttaagat gagaaaaatt ctgtgacgct tgccaacatt tctgatgatt agcattccct
    50401 tcgccatttc cctgagcaaa ctttagctat tcttatcaat tatgcttatg ggagatctac
    50461 agatgatgcc tacgcttgct ccaccatctg tcctttcggc tccccagcgc cgctgccaga
    50521 tcttgctgac gctctttcag ccggggttga ccgccaccat ggcaaccttc agcgagctta
    50581 atggtgtgga tgatgatatt gccagtcttg atatcagcga aacaggacgg gagatcctgc
    50641 gctatcatca actcacactg acgactggtt atgacggtag ctaccgggtt gaaggtacag
    50701 tgcttaacca acgtttgtgt ttatttcact ggctacgacg tggtttccgt ctgtgtccgt
    50761 catttattac cagccaattc acccccgccc tgaagagtga actgaagcgg cgcggaattg
    50821 cgcgtaactt ttacgacgat accaatctac aagcgttagt gaatctctgc tcccgacggc
    50881 tgcaaaaacg ctttgaaacg cgcgatattc atttcctgtg tctgtatctg caatattgtt
    50941 tgctgcaaca ccacgctgga attacgcctc agtttaatcc gctccaacgt cgctgggcgg
    51001 aatcctgcct tgaatttcag gtagcgcagg aaattggacg ccactggcag cgtcgggcgc
    51061 tccagcctgt accgcctgat gagccactgt ttatggcatt acttttttcc atgttgcggg
    51121 ttcccgatcc attgcgtgat gcgcatcagc gagacagaca attgcgtcag tctatcaaac
    51181 gtctggtaaa ccattttcgt gagctgggaa atgttcgttt ttatgatgaa caggggttat
    51241 gcgatcagct ttatacccac ctcgcccagg cgttaaatcg cagtttgttt gccatcggta
    51301 ttgataatac cctgccggaa gagttcgcca gactgtaccc acgcctggtg cgcaccaccc
    51361 gcgcggcgct ggccggattt gaaagtgaat acggcgtcca tctttctgat gaggaaagcg
    51421 gtctggtcgc ggtgattttc ggtgcctggc taatgcagga aaacgatctg catgaaaaac
    51481 agattattct actgaccggg aatgatagcg agcgagaagc gcagattgag cagcagttac
    51541 gcgaactaac gttactgccg ctcaacatta agcatatgtc ggtaaaggca tttttgcaga
    51601 caggcgctcc gcgtggcgcg gcactgatta ttgcgcctta taccatgcca ttaccgctct
    51661 tttcaccacc gctgatctat acggacctga cgctgacaac acatcaacag gagcagatcc
    51721 gcaaaatgct cgaatcagca tgagggaaca actttcgggc gcaaaaacat tgccggaagc
    51781 gccaccagcg ccattaccca gaacacgccg tggcccagat attgatacag gaaaccggcg
    51841 aaaacggtca tgatagcgat actgccgccc atcgcgacgg cagagtaaac cgcctgtaaa
    51901 cggatgactt cgctaccctg gcgaacagca atatagcgca tggcggccag gtggcagacc
    51961 gtgaaggtgc cgcaatgcaa aatttgcacc actatcaacc acggcaacgc cgtagttgct
    52021 cccataatgc cccagcgcac tacgccgcaa atcgccgaga tcaacagcat atcgcgagca
    52081 ctacaacggc ggaaaagttt attactcagc gcaaagataa tgacttccgc caccacgccc
    52141 agcgaccaca aataccccac cgccgaggcc gagtagccag ctgcctgcca gtaaatggcg
    52201 ctaaaaccgt aataggccgc atgtgccccc tgcaataaac aaacgcaggc cagaaagcgc
    52261 cagttctggc gaaccagcgc caaccacgca gaccaaccgg tgctctcctg ctggcgactt
    52321 gccccttgtg gctgaatcgt cggacggatg agaaagccga gcagcatgga tgccacgccc
    52381 aacgtcaaca gcgcgaggat cacccgataa tcaaacatag tgaccagttt gcccgtcagc
    52441 gccgagccaa tgacaaacgc caccgagccc cacagtcgca ctttgccgta atcaagcggg
    52501 aactgctttt gccacgtatt cgccagtgca tcggtcaacg gtaccagcgg tgagaaaaag
    52561 aggttaaagc caatcatcac cagcatcagc cacgctacgt gcgcccccgc ccagaaggcg
    52621 acagcaaaga gaagtgtcag cagtgccagc acgcgcaagg cggaaatcag gcgggaagga
    52681 tcgctgacgc ggggcgcgat gagcaaactc ccgagaaaac gggcaaccag acctgccccc
    52741 aataacaggc cgatggtttc tggcgttaaa ccaatccctt taagccagac gctccagaaa
    52801 ggtagaaaaa tgccgtaact aaaaaagtat gtgaaatagc cgagcgccaa ccagcgcgtg
    52861 gattgcaaaa ccatgagtcc ctcccgtcag gaggcgttag tctggggtta atcgaatgtt
    52921 ggtgcaagtt gaaaaataag aatattcgag cctgcttccc gatgtctgta acgccagttc
    52981 acaagcacta aaaaactgcg atccgcgacg catgttgcaa aaataatgcg taattatgcc
    53041 gttacgcttg ccaaacgttc ctgcacaatg gcgatgaaat cgcgtaatgc cggcttcatc
    53101 tcccctttct tccacgccat cagtaaagca atggaaggca catttccggc aatgggacga
    53161 aaaacaacct gcccggtatt aaaattattc atataaccgg gtatcaaagt gacgcccagc
    53221 cccatgccca ccagattcat ggtcaccaga atattcgttg ccacctggac gatatttggc
    53281 tggctgtttt cttgcgcaaa ccacgcttta acgatcggcg caagcgaacc ggaatacgcc
    53341 ggatcggtac tgacgaaatt cacgccatcc agttgcgcgg cggtgatctc tttttcatgt
    53401 gctaaagggt gatcaaccgg taacaccacc actaatggtt cgtcaaaaag ctccagataa
    53461 tcaatctccg ggctataaac aggatggcgc attaagccga cgtcgagttc accacggcga
    53521 attttttcct cctgttgcgt ggtgattaaa ctcaccagct caatcaaggt gtctggctgt
    53581 ctgagacgaa acattggtaa tacttttggc aataaattca cttccgccga tggcacaaag
    53641 ccaatggtta attgtctgtc ttcctgaaca attttccgcg cccgtaattt ggcattttcc
    53701 gcttgttcga ggattgccag cgcgtcctgg agaaaacatt ctcccgccgc cgtcaacgcg
    53761 actttgcgct tatccctcac cagtaacgga acaccgacac agttttcaag atcgcggatc
    53821 tggctgctta acgaaggctg tgaggtatgc agtttttccg ccgcacgggt aaagttcagt
    53881 gcctgcgcca ctgcgacgaa atagcgtaaa tgccgtagtt ccatcacctt ccccttgtta
    53941 tcgaaaaaac gtctcagctg gtagaaatta actatttcac atattagcaa ccaaccaaca
    54001 acatccttat gacacaaaaa tagaaggtca atacatctta tctttcagga ttaaaaaata
    54061 tgaccacacc ctcagattta aacatttacc aactgattga tacccaaaat ggtcgggtca
    54121 ctccgcgtat ttataccgac ccagatattt accaactgga gcttgaacgt attttcggcc
    54181 gttgctggtt atttctcgcc cacgaaagcc agatcccaaa acctggtgat ttctttaaca
    54241 cctacatggg ggaagatgcg gttgtcgtag tgcgtcagaa agacggcagc attaaggcgt
    54301 ttctcaacca atgccgccac cgggccatgc gtgtgagtta tgcagattgc ggcaacactc
    54361 gcgcctttac ctgtccgtat cacggctggt cttatggcat taacggcgag ttgatcgatg
    54421 taccgctgga acctcgcgcc tacccacaag ggttgtgtaa atcccactgg ggactaaacg
    54481 aagttccttg tgtggagagt tataaagggc tgatttttgg caactgggat accagcgcac
    54541 cgggcctgcg tgattacctg ggtgacattg cctggtatct ggatggcatg ctggatcgtc
    54601 gcgaaggcgg caccgaaatt gtcggcggcg tacaaaagtg ggtgatcaac tgtaactgga
    54661 aattcccggc agagcagttc gccagtgacc agtatcatgc tctgttcagc catgcttctg
    54721 ccgttcaggt attaggggcg aaagatgatg gcagcgataa gcgcctcggt gatggacaaa
    54781 ccgcccgccc ggtgtgggaa accgccaaag atgcgctgca atttggtcag gacggtcacg
    54841 gtagcggttt cttctttact gaaaaaccgg atgctaatgt ctgggtcgat ggcgcagttt
    54901 caagctatta ccgcgaaacc tatgccgaag cagaacaacg tttaggtgaa gttcgcgccc
    54961 tgcgcctggc gggtcataac aatattttcc ccacgctttc atggctcaac ggcactgcca
    55021 cgctccgcgt ctggcatccg cgcggccctg atcaagttga agtgtgggcg ttctgtatta
    55081 ctgacaaagc cgcctccgat gaagttaaag ccgcttttga aaacagcgcc actcgtgctt
    55141 ttggtcctgc tggttttctc gagcaggatg actcggagaa ctggtgtgaa atccagaaat
    55201 tgcttaaagg ccaccgcgcc cgcaacagca aactgtgtct ggaaatgggg cttggtcagg
    55261 aaaagcgccg cgacgacggc attcctggca ttactaacta tatcttttca gaaactgccg
    55321 ctcgtggaat gtaccaacgc tgggccgatc ttctgagtag cgaaagctgg caggaagtgc
    55381 tcgataaaac cgccgcttac cagcaggagg tgatgaaatg agtgcgcaag tttcactaga
    55441 gttacatcac cgcattagcc agtttctctt tcacgaagcc agcttactgg acgactggaa
    55501 atttcgtgac tggctggagc agctcgacaa agagattcgt tacaccatgc gcaccacagt
    55561 taacgcgcaa acacgcgacc gccgcaaagg cgtccagcca ccgacaacct ggatttttaa
    55621 tgacaccaaa gaccagctgg agcggcgaat cgcccgtctg gaaacgggca tggcctgggc
    55681 agaagagccg ccgtcacgca cccgtcactt aatcagcaac tgccagataa gcgaaaccga
    55741 catcccaaac gtatttgctg tgcgggtaaa ttatctgctt tatcgggcac aaaaagagcg
    55801 cgatgaaaca ttctatgttg gaacgcgttt cgacaaagtt cgccgtctgg aagatgacaa
    55861 ctggcgcttg ctggaacggg atatcgtcct ggatcaagcg gtaatcactt cccataacct
    55921 gagtgtactg ttctgatgaa tcgaatttat gcgtgtcccg ttgcggatgt gccggagggt
    55981 gaggctctcc ggatcgatac ctcgcccgtc atcgccctgt tcaacgttgg cggcgagttt
    56041 tatgccatta acgatcgttg cagccatggt aatgcgtcga tgtcagaagg gtatctggaa
    56101 gatgacgcca cggtggagtg cccgctacac gccgccagtt tttgcctgaa aacggggaaa
    56161 gcgttatgcc tgcccgccac cgatccgctc accacttatc cagtacacgt tgaaggtggt
    56221 gacattttca tcgacttacc ggaggcgcag ccatgagcga tctgcataac gagtccattt
    56281 ttattaccgg cggcggatcg ggattagggc tggcgctggt cgagcgattt atcgaagaag
    56341 gcgcgcaagt tgccacgctg gaactgtcgg cggcaaaagt cgccagtctg cgtcagcgat
    56401 ttggcgaaca tattctggcg gtggaaggta acgtgacctg ttatgccgat tatcaacgcg
    56461 cggtcgatca gatcctgact cgttccggca agctggattg ttttatcggc aatgcaggca
    56521 tctgggatca caatgcctca ctggttaata ctcccgcaga gacgctcgaa accggcttcc
    56581 acgagctgtt taacgtcaac gtactcggtt acctgctggg cgcaaaagcc tgcgctccgg
    56641 cgttaatcgc cagtgaaggc agcatgattt tcacactgtc aaatgccgcc aggtatcctg
    56701 gcggcggtgg cccgctgtac accgccagta aacatgccgc aaccggactt attcgccaac
    56761 tggcttatga actggcaccg aaagtgcggg tgaatggcgt cggcccgtgt ggtatggcca
    56821 gcgacctgcg cggcccacag gcgctcgggc aaagtgaaac ctcgataatg cagtctctga
    56881 cgccggagaa aattgccgcc attttaccgc tgcaattttt cccgcaaccg gcggatttta
    56941 cggggccgta tgtgatgttg gcatcgcggc gcaataatcg cgcattaagc ggtgtgatga
    57001 tcaacgctga tgcgggttta gcgattcgcg gcattcgcca cgtagcggct gggctggatc
    57061 tttaaggaag cacgatgaaa gaaaaaacga tcattattgt cggtggcggg caagcggcgg
    57121 caatggctgc ggcctcgcta cgccagcaag ggttcaccgg tgagctgcat ctgttttccg
    57181 atgagcgaca tcttccttat gaacgacctc cactctcgaa atccatgttg ctggaagatt
    57241 ccccgcagtt acagcaggtg ttacccgcta actggtggca ggaaaacaat gttcatctgc
    57301 attccggtgt aaccatcaaa acgctgggtc gcgacacacg agagttagtg ttaaccaacg
    57361 gcgaaagctg gcactgggat cagcttttta tagcaatcgg cgcggcagct cgaccgctgc
    57421 cgttgcttga tgcactggga gaacgctgct ttaccctacg ccatgccggt gatgccgcca
    57481 gactgcgaga agttctgcag cccgaacggt cagtcgtgat tgtcggtgcc ggaactattg
    57541 gtctggaact ggctgccagc gccacgcagc gcagatgtaa ggtgacagtg attgaactgg
    57601 cggcaaccgt catgggccgt aatgcaccac cgcccgtgca acgctatctt ttacagcgcc
    57661 accagcaggc tggtgtgcgc attctgctca ataatgccat tgaacatgtg gtcgatggtg
    57721 aaaaagtaga actgacgctg caaagtgggg aaacgcttca ggctgatgtg gtgatttacg
    57781 gtattggtat cagcgccaac gagcaactgg ctcgcgaggc caaccttgat actgccaatg
    57841 gcattgtcat tgatgaggct tgccgcacct gcgatcccgc gatctttgcc ggtggcgatg
    57901 tggcaatcac tcgtcttgat aatggtgcac tacaccgctg cgaaagctgg gaaaacgcca
    57961 ataaccaggc gcaaattgcc gctgccgcaa tgttagggct accgctaccg ctactgccgc
    58021 cgccgtggtt ctggagcgat cagtacagtg ataacttaca gtttattggc gatatgcgtg
    58081 gcgatgactg gctttgtcgt ggcaacccgg aaactcagaa ggcgatttgg tttaatctgc
    58141 aaaacggcgt gcttatcggt gcggtcacgc tgaatcaggg gcgtgagatt cgcccaattc
    58201 gcaaatggat ccagagcggc aaaacgtttg atgcgaaact gctgatagat gagaacatcg
    58261 cgcttaaatc actgtaacca ggataattag cgaatatctc aacgcctggg gcgtggcgag
    58321 gtgcaagagt gtgtattacg tttaaatcac attatcttgc aaagggattg gttatgaaca
    58381 cacta
//
LOCUS       DAJVQG010000008        57420 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJVQG010000008 DAJVQG010000000
VERSION     DAJVQG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.008"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            145..1488
                     /gene="argG"
                     /locus_tag="OQM83_000454"
     CDS             145..1488
                     /gene="argG"
                     /locus_tag="OQM83_000454"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCS1518807.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1496..3103)
                     /locus_tag="OQM83_000455"
     CDS             complement(1496..3103)
                     /locus_tag="OQM83_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCS1518808.1"
                     /translation="MLEDFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFFVLTILV
                     VKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDPDEVVKM
                     LGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQFAYKDA
                     KNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVRDTGIDT
                     YVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQSISGAPYTALSVPLSLTAD
                     SVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETVYVRGFD
                     ELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDACYDNSIH
                     YTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHVPMFIWY
                     SPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDSRVVDAN
                     HDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(3581..3667)
                     /locus_tag="OQM83_000456"
     tRNA            complement(3581..3667)
                     /locus_tag="OQM83_000456"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3631..3633),aa:Leu,seq:gag)
     gene            complement(3682..4014)
                     /gene="secG"
                     /locus_tag="OQM83_000457"
     CDS             complement(3682..4014)
                     /gene="secG"
                     /locus_tag="OQM83_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCS1518809.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4242..5579)
                     /gene="glmM"
                     /locus_tag="OQM83_000458"
     CDS             complement(4242..5579)
                     /gene="glmM"
                     /locus_tag="OQM83_000458"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCS1518810.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5572..6420)
                     /gene="folP"
                     /locus_tag="OQM83_000459"
     CDS             complement(5572..6420)
                     /gene="folP"
                     /locus_tag="OQM83_000459"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCS1518811.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6510..8444)
                     /gene="ftsH"
                     /locus_tag="OQM83_000460"
     CDS             complement(6510..8444)
                     /gene="ftsH"
                     /locus_tag="OQM83_000460"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCS1518812.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8544..9173)
                     /gene="rlmE"
                     /locus_tag="OQM83_000461"
     CDS             complement(8544..9173)
                     /gene="rlmE"
                     /locus_tag="OQM83_000461"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCS1518813.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9299..9592
                     /gene="yhbY"
                     /locus_tag="OQM83_000462"
     CDS             9299..9592
                     /gene="yhbY"
                     /locus_tag="OQM83_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCS1518814.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9748..10224)
                     /gene="greA"
                     /locus_tag="OQM83_000463"
     CDS             complement(9748..10224)
                     /gene="greA"
                     /locus_tag="OQM83_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCS1518815.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10472..11905
                     /gene="dacB"
                     /locus_tag="OQM83_000464"
     CDS             10472..11905
                     /gene="dacB"
                     /locus_tag="OQM83_000464"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCS1518816.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(12091..13263)
                     /gene="cgtA"
                     /locus_tag="OQM83_000465"
     CDS             complement(12091..13263)
                     /gene="cgtA"
                     /locus_tag="OQM83_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCS1518817.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13279..14244)
                     /locus_tag="OQM83_000466"
     CDS             complement(13279..14244)
                     /locus_tag="OQM83_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1518818.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14371..14628)
                     /gene="rpmA"
                     /locus_tag="OQM83_000467"
     CDS             complement(14371..14628)
                     /gene="rpmA"
                     /locus_tag="OQM83_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCS1518819.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14649..14960)
                     /gene="rplU"
                     /locus_tag="OQM83_000468"
     CDS             complement(14649..14960)
                     /gene="rplU"
                     /locus_tag="OQM83_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCS1518820.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15219..16190
                     /gene="ispB"
                     /locus_tag="OQM83_000469"
     CDS             15219..16190
                     /gene="ispB"
                     /locus_tag="OQM83_000469"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCS1518821.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16419..16697
                     /gene="sfsB"
                     /locus_tag="OQM83_000470"
     CDS             16419..16697
                     /gene="sfsB"
                     /locus_tag="OQM83_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCS1518822.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16745..18004)
                     /gene="murA"
                     /locus_tag="OQM83_000471"
     CDS             complement(16745..18004)
                     /gene="murA"
                     /locus_tag="OQM83_000471"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCS1518823.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18059..18313)
                     /gene="ibaG"
                     /locus_tag="OQM83_000472"
     CDS             complement(18059..18313)
                     /gene="ibaG"
                     /locus_tag="OQM83_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCS1518824.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18473..18766)
                     /gene="mlaB"
                     /locus_tag="OQM83_000473"
     CDS             complement(18473..18766)
                     /gene="mlaB"
                     /locus_tag="OQM83_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCS1518825.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18766..19401)
                     /gene="mlaC"
                     /locus_tag="OQM83_000474"
     CDS             complement(18766..19401)
                     /gene="mlaC"
                     /locus_tag="OQM83_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCS1518826.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19420..19971)
                     /gene="mlaD"
                     /locus_tag="OQM83_000475"
     CDS             complement(19420..19971)
                     /gene="mlaD"
                     /locus_tag="OQM83_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCS1518827.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19976..20758)
                     /gene="mlaE"
                     /locus_tag="OQM83_000476"
     CDS             complement(19976..20758)
                     /gene="mlaE"
                     /locus_tag="OQM83_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCS1518828.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLANCLIKSVVFAITVTWISL
                     FNGYDTIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20766..21575)
                     /gene="mlaF"
                     /locus_tag="OQM83_000477"
     CDS             complement(20766..21575)
                     /gene="mlaF"
                     /locus_tag="OQM83_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCS1518829.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21785..22762
                     /locus_tag="OQM83_000478"
     CDS             21785..22762
                     /locus_tag="OQM83_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCS1518830.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22776..23762
                     /gene="kdsD"
                     /locus_tag="OQM83_000479"
     CDS             22776..23762
                     /gene="kdsD"
                     /locus_tag="OQM83_000479"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCS1518831.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23783..24349
                     /gene="kdsC"
                     /locus_tag="OQM83_000480"
     CDS             23783..24349
                     /gene="kdsC"
                     /locus_tag="OQM83_000480"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCS1518832.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24346..24921
                     /gene="lptC"
                     /locus_tag="OQM83_000481"
     CDS             24346..24921
                     /gene="lptC"
                     /locus_tag="OQM83_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCS1518833.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPNSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24890..25447
                     /gene="lptA"
                     /locus_tag="OQM83_000482"
     CDS             24890..25447
                     /gene="lptA"
                     /locus_tag="OQM83_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCS1518834.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25454..26179
                     /gene="lptB"
                     /locus_tag="OQM83_000483"
     CDS             25454..26179
                     /gene="lptB"
                     /locus_tag="OQM83_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCS1518835.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26227..27660
                     /gene="rpoN"
                     /locus_tag="OQM83_000484"
     CDS             26227..27660
                     /gene="rpoN"
                     /locus_tag="OQM83_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCS1518836.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27683..27970
                     /gene="hpf"
                     /locus_tag="OQM83_000485"
     CDS             27683..27970
                     /gene="hpf"
                     /locus_tag="OQM83_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCS1518837.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28088..28579
                     /gene="ptsN"
                     /locus_tag="OQM83_000486"
     CDS             28088..28579
                     /gene="ptsN"
                     /locus_tag="OQM83_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCS1518838.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28625..29479
                     /gene="rapZ"
                     /locus_tag="OQM83_000487"
     CDS             28625..29479
                     /gene="rapZ"
                     /locus_tag="OQM83_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCS1518839.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29476..29748
                     /gene="npr"
                     /locus_tag="OQM83_000488"
     CDS             29476..29748
                     /gene="npr"
                     /locus_tag="OQM83_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCS1518840.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29962..30594
                     /gene="yrbL"
                     /locus_tag="OQM83_000489"
     CDS             29962..30594
                     /gene="yrbL"
                     /locus_tag="OQM83_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCS1518841.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30591..31319)
                     /gene="mtgA"
                     /locus_tag="OQM83_000490"
     CDS             complement(30591..31319)
                     /gene="mtgA"
                     /locus_tag="OQM83_000490"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCS1518842.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFSEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31316..31969)
                     /gene="elbB"
                     /locus_tag="OQM83_000491"
     CDS             complement(31316..31969)
                     /gene="elbB"
                     /locus_tag="OQM83_000491"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCS1518843.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32199..34535)
                     /gene="arcB"
                     /locus_tag="OQM83_000492"
     CDS             complement(32199..34535)
                     /gene="arcB"
                     /locus_tag="OQM83_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCS1518844.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPVDVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34631..35560)
                     /locus_tag="OQM83_000493"
     CDS             complement(34631..35560)
                     /locus_tag="OQM83_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCS1518845.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36235..40695
                     /gene="gltB"
                     /locus_tag="OQM83_000494"
     CDS             36235..40695
                     /gene="gltB"
                     /locus_tag="OQM83_000494"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCS1518846.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40708..42126
                     /gene="gltD"
                     /locus_tag="OQM83_000495"
     CDS             40708..42126
                     /gene="gltD"
                     /locus_tag="OQM83_000495"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCS1518847.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42310..42789
                     /locus_tag="OQM83_000496"
     CDS             42310..42789
                     /locus_tag="OQM83_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCS1518848.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            42806..43111
                     /locus_tag="OQM83_000497"
     CDS             42806..43111
                     /locus_tag="OQM83_000497"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1518849.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43187..43651)
                     /locus_tag="OQM83_000498"
     CDS             complement(43187..43651)
                     /locus_tag="OQM83_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCS1518850.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(43648..44523)
                     /gene="nanK"
                     /locus_tag="OQM83_000499"
     CDS             complement(43648..44523)
                     /gene="nanK"
                     /locus_tag="OQM83_000499"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCS1518851.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44520..45209)
                     /locus_tag="OQM83_000500"
     CDS             complement(44520..45209)
                     /locus_tag="OQM83_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCS1518852.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45257..46747)
                     /gene="nanT"
                     /locus_tag="OQM83_000501"
     CDS             complement(45257..46747)
                     /gene="nanT"
                     /locus_tag="OQM83_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCS1518853.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(46857..47750)
                     /gene="nanA"
                     /locus_tag="OQM83_000502"
     CDS             complement(46857..47750)
                     /gene="nanA"
                     /locus_tag="OQM83_000502"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCS1518854.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(47872..48663)
                     /gene="nanR"
                     /locus_tag="OQM83_000503"
     CDS             complement(47872..48663)
                     /gene="nanR"
                     /locus_tag="OQM83_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCS1518855.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            49043..50365
                     /gene="dcuC"
                     /locus_tag="OQM83_000504"
     CDS             49043..50365
                     /gene="dcuC"
                     /locus_tag="OQM83_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCS1518856.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(50408..50905)
                     /gene="sspB"
                     /locus_tag="OQM83_000505"
     CDS             complement(50408..50905)
                     /gene="sspB"
                     /locus_tag="OQM83_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCS1518857.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(50911..51549)
                     /gene="sspA"
                     /locus_tag="OQM83_000506"
     CDS             complement(50911..51549)
                     /gene="sspA"
                     /locus_tag="OQM83_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCS1518858.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMCLGRS"
     gene            complement(51944..52336)
                     /gene="rpsI"
                     /locus_tag="OQM83_000507"
     CDS             complement(51944..52336)
                     /gene="rpsI"
                     /locus_tag="OQM83_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCS1518859.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(52352..52780)
                     /gene="rplM"
                     /locus_tag="OQM83_000508"
     CDS             complement(52352..52780)
                     /gene="rplM"
                     /locus_tag="OQM83_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCS1518860.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(52999..54126)
                     /gene="zapE"
                     /locus_tag="OQM83_000509"
     CDS             complement(52999..54126)
                     /gene="zapE"
                     /locus_tag="OQM83_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCS1518861.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            54320..54718
                     /locus_tag="OQM83_000510"
     CDS             54320..54718
                     /locus_tag="OQM83_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCS1518862.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            54872..56239
                     /gene="degQ"
                     /locus_tag="OQM83_000511"
     CDS             54872..56239
                     /gene="degQ"
                     /locus_tag="OQM83_000511"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCS1518863.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            56329..57396
                     /gene="degS"
                     /locus_tag="OQM83_000512"
     CDS             56329..57396
                     /gene="degS"
                     /locus_tag="OQM83_000512"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCS1518864.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT        14262 a        15301 c        14250 g        13607 t
ORIGIN      
        1 gcgcattatt ttacaaaaaa cacactaaac tcttcctgtc tccgataaaa gatgattaaa
       61 tgaaaactca tttattttgc ataaaaattc agtgagagcg gaaatccagg ctcatcatca
      121 gttaattaag cagggtgtta ttttatgacg acgattctca agcatctccc ggtaggtcaa
      181 cgtattggta tcgctttttc cggcggtctg gacaccagtg ccgcactgct gtggatgcga
      241 caaaagggag cggttcctta tgcatatact gcaaacctgg gccagccaga cgaagaggat
      301 tatgatgcga tccctcgtcg tgccatggaa tacggcgcgg agaacgcacg tctgatcgac
      361 tgccgcaaac aactggtggc cgaaggtatt gccgctattc agtgtggcgc atttcataac
      421 accaccggcg gcctgaccta tttcaacacg acgccgctgg gccgcgccgt gactggtacc
      481 atgctggttg ctgcgatgaa agaagatggc gtgaatatct ggggtgacgg tagcacctat
      541 aaaggaaacg atatcgaacg tttctatcgt tatggtctgc tgaccaatgc tgaactgcag
      601 atttacaaac cgtggcttga tactgacttt attgatgaac tgggcggccg tcatgagatg
      661 tctgaattta tgattgcctg cggtttcgac tacaaaatgt ctgtcgaaaa agcctactcc
      721 acagactcca acatgcttgg tgcaacgcac gaagcgaagg atctggaata cctcaactcc
      781 agcgtcaaaa tcgtcaaccc aattatgggc gtgaaattct gggatgagag cgtgaagatc
      841 ccggcagaag aagtcacagt acgctttgag caaggtcatc cggtagcgct gaacggtaaa
      901 acctttagcg acgacgtaga aatgatgctg gaagctaacc gcatcggcgg tcgtcacggc
      961 ctgggcatga gcgaccagat tgaaaaccgt atcatcgaag cgaaaagccg tggtatttac
     1021 gaagctccgg ggatggcact gctacatatc gcttatgaac gtctgttgac cggtattcac
     1081 aacgaagaca ccattgagca gtatcatgca catggtcgtc agttgggccg tctgctgtac
     1141 caggggcgtt ggtttgattc ccaggcgctg atgctgcgtg actctctgca acgctgggtt
     1201 gccagccaga tcactggtga agttaccctg gagctgcgcc gtgggaacga ttattcaatc
     1261 ctgaataccg tctcagagaa cctgacctac aagccagagc gtctgacgat ggaaaaaggc
     1321 gactcggtgt tctcgccaga tgatcgtatt ggtcaattga ccatgcgtaa cctggatatc
     1381 actgataccc gagagaaact ttttggttat gccaaaactg gcctgctctc ctcctctgcc
     1441 acttcaggcg tgccgcaggt ggagaatctg gaaaacaaag gccagtaatt cgacatcaac
     1501 cctgcccttc aggggtgggg ttaccttgct tatcctctgt aaactccttt ctgagcatca
     1561 cataatcgaa aacatcatgg tttgcatcca caacccgtga gtctccttta tagtgtgcaa
     1621 tcacttcctc cagcgttttc ggttgttccg gctttgttac ccccatccac gcattaataa
     1681 ggtaattatt gtaagctgtc gaaaagatgt tgttttccgt tgtacgatcc acgccatccc
     1741 caagaacggg gctataccag ataaacatcg ggacatgata tgcctgctgg ctagcctccc
     1801 tgcctccatg aaaatagacg ttcttcttcg tagggtcacg ttccaggccg tggtcggcaa
     1861 aatacatgac cgaggcgcgg cgatctttta ataattcaaa aacctgacct agcaaactat
     1921 ctgtgtaatg aatggagttg tcatagcagg catcctgatc gtcctgcggt tgaaacacgg
     1981 cgctggattg cggataggcg ctacaagccg gttcatggct tccatttaaa tgaagaacaa
     2041 tcagtttctt ttgctgcgta ttttgctgta atgcttgcga taaatgcggc aacaacaatt
     2101 catcaaatcc tctgacataa actgtttcca tggcgcgcat ggcgatactg gtaactgctg
     2161 taccattctg ccgaaaagcg gattgcgagc ttagccagaa agtctgaaat cctgcttgat
     2221 tagccatatt aataatgttg tcggggtaat tatgaatgtc atgactcaaa acagaatcag
     2281 cagttaaaga aaggggaacc gacagcgcag tgtaaggtgc gccgcttatt gattgattaa
     2341 acagtttgat ctgttttctt tgtgcttcaa cttgcggtgt cgtagagcgt gtatatccat
     2401 acaaagacat attgtcgaca cgtacagact cccccacaat caacacgtag gtatcaatac
     2461 ctgtatccct gactgataat tgaaaatacg gcaccgtgtt tgcaattgag agtaatcttt
     2521 gatgctcttt cgctgctaaa gcaaaataat tgagattgaa aaacggcgta taggtagcaa
     2581 atcgcgacgc tagtatatat ggactgaatg cttttttatt ttttgcatct ttataagcaa
     2641 attgacaagc agaaaataaa ctgcccgaaa tgacaatcag caataatatt cctgtcactt
     2701 tttttgtcgg caaggaaaca tcatatttta ttattactgc caaaaaaaga agggataaaa
     2761 aagcaaaagc acatagataa gggatataca tccccagcat tttgacaact tcatccggat
     2821 cactctgcag cacactaatc gcaaatccat cattgaaagt tgtaccaaag gtacaccatg
     2881 aatagaggct aatactcatg tctgccgcag tcagtaacac aaatggtgcg gcaacaagca
     2941 ggcgaagcgg aagggatgat atacgtttta caaccaggat ggtcaggacg aagaacagca
     3001 atcgctgaac catatgcgcg ccggaagagg cgactaagtt cgtaatacca aaaacaataa
     3061 cacaaagata caacaaccaa tgagatttaa aatcttctag caaatttgtt gaatactgtc
     3121 atccagaaat ccaagttcac aaccaatata aacaattatc aacaatgttc aattttatat
     3181 cagacatgtt tttcaactct gatccatatc agaccgaaac actgttagtt ttttcttttt
     3241 aaatgatgcg gccattgcgt tctcagtaat taaataacat tatcaatgcg tctctaaaca
     3301 ttacgcagaa cgtagccatc agtagttata ctgacaggag caggatggtt tatcgagaag
     3361 taaacatatc cgtattcgta ccggaactgg agttgacaca ataaagtgcc aattatgtca
     3421 gcagaaggga aaagtttata actaaggcgt accggcacca tcgtttcaag gtaccagcta
     3481 cgagtaaagc aactggacga gatacagata cctgacaacc attcctcaga ccaggaccaa
     3541 aacgaaaaaa gacgcttttc agcgtctctt ttctggaatt tggtaccgag gacgggactt
     3601 gaacccgtaa gccctattgg gcactaccac ctcaaggtag cgtgtctacc aattccacca
     3661 cctcggcacg gatactactt tttagttcgg gatatcgctg gtcggcttag ccggagcagc
     3721 tggctgagtt tgttcggttt tcgccggtgc actcagattt tcccattcgc tacctttatt
     3781 ggttttgttg ctattgatgt tacccagcac cagactgatg ataaagaata acgttgccag
     3841 cagcgccgtc atgcgggtca tgaagttacc agaaccactt gaaccaaaca gcgtagcgga
     3901 agcgcctgct ccgaaggagg ctcccatatc agcgccttta ccttgctgca gcatgatcag
     3961 accaacaagg ccaattgcca caataaggaa aactaccaaa agagcttcgt acataatcaa
     4021 cctgttcctt gcggagttgc cgcgtaccaa tgcttcaacc aataaagcgg gagtttttaa
     4081 tctttcccac tgaagtgggt gtgaatacta accaaagcga atgaccttcg caagggcaat
     4141 tttatcgcat tgtatcaact gcggaaaaaa acagcaaaac cgatgtgttc gctgagaaaa
     4201 aaggcgacaa tcgccgcctt tttagccagt tatttaccgt tttaaacggc ttttactgca
     4261 tcggcgatac ggtgtgcaaa ttcagttacc tgcgcttcgt cttcaccttc taccatcacg
     4321 cgaattaacg gttcggtgcc ggatttacgc aacagcacac gtccacggct acccagcgcc
     4381 gcttcaactt cggcggtcac agctttaact gactcatgct caagcggatc gccgctacct
     4441 gcggtgtaac gcacgttaac cagaatctgc gggaacattt tcatgccgct gcaaaggtcg
     4501 tgcaggctca tatggttacg tgccatcgcc gccagcacct gcaagccagc aacgatgccg
     4561 tcaccggtag tggttttatc cagcaggatc acatgaccgg aattctctgc accgatacgc
     4621 cagcctttct cctgcatttt ttccagtacg tagcggtcac ccactttcgc gcgcgcaaat
     4681 ggaattccta actgtttcaa cgccagctca agccccatgt tactcatcaa tgtacccaca
     4741 gcaccaccac gcagctggcc ctgacgaaga ccttcacgcg cgatgatata catgatctga
     4801 tcaccatcga ctttattgcc ttcatggtca accataatca cgcgatcgcc gtcgccgtcg
     4861 aaggcaatac cgagatccgc tttttcagcc agcacacgag cctggagcgc gcgaacgtcg
     4921 gtagccccca cttcggcatt gatgtttaca ccgttcggct cacaaccgat agcgataacg
     4981 ttcgccccca gttcacgcag cacgttcggc gcgatgtgat aagtcgcacc gtttgcgcaa
     5041 tccaccacaa tcttcagttc gctgaggcta agttcgttcg ggaacgtggc tttgcaaaac
     5101 tcgatatagc gacccgcagc atcaacgata cggctggctt tacccagttc tgccgaatca
     5161 acacagctga tctctttttc catttccgct tcgatggcct cttctaccgc atccggtagt
     5221 ttggtgccgt cgatagagaa aaatttaatg ccattatcgt agaacgggtt atgcgatgca
     5281 gatatcacaa ttccggcctc tgcgcggaag gtacgcgtca gataagccac ggccggtgtt
     5341 ggcatcgggc cagtgaagag tgcggaaagg cccgctgccg ccagacccgc ttccagtgct
     5401 gactccagca tatagccaga aatacgcgtg tctttaccaa taatgatctt acgggagccg
     5461 tggcgcgcca gcactttacc cgcggcccaa cccagcttaa gcacaaaatc aggtgtgatc
     5521 ggcgcatccc ctacacgacc acgaatccca tcggtaccga aatatttacg attactcata
     5581 gcgtttgttt tcctttgcag acagagtggc ttccaccacc cgcatcgctt ctacggtttc
     5641 tttgacgtca tgaacacgaa taatgtgcgc gccttgcatt gcggcaatga ccgcacaggc
     5701 cagactaccg ctcaggcgct cggacggccc cacgttcagc agctgcccaa tcatcgattt
     5761 tcgtgacata cctaccaaca gcggcaggtt gaaatggtga aattcagcca ggcgcgccag
     5821 taatgaatag ttatgggaga gatttttacc gaaaccgaat ccggggtcga gcaacaattt
     5881 ctcttttgcg atacccgcct gctcgcaacg tgctatttgc tcaataaagt agcgattcac
     5941 ttctgcaaag acatcgtcat acttcggagc ttcctgcatg gtttttggat ttccctgcat
     6001 atgcatcaga caaaccggta aaccggtttc tgcagccgcc tccagagcgc caggttcgga
     6061 aagggagcgg atatcgttaa taatgtgagc gccaactttc gctgactcac ggatgacttc
     6121 tggtttggat gtatctaccg agatccagac ttcgaagcgt tgagcaattg cctcaaccac
     6181 aggaataaca cgttgcaact cttcttcaac gctaacttcc gccgcccctg ggcgcgtgga
     6241 ctcgccacca acgtcaatga tcgtcgcgcc agcgttgatc atcagattcg catgtttcac
     6301 cgcatctatc agcgagttat gcgtgccacc atccgaaaag gaatcaggcg tgacgttgag
     6361 gatccccatt acgtgaggat ggctaaggtc cagtgaagtg ccctgggcaa agagtttcat
     6421 ggtgttatcc ctggtatgaa ttgataagaa aaaaccccgg agcacgcccc ggggttttcg
     6481 gtacaaatac agtcatctga tgcgggaact tacttgtcgc ctaactgctc tgacatggtg
     6541 ttacccgggt tcggcgtacg cggttcatca accggacgag gagcctttgg actaccattg
     6601 tcgccagaat tgttagaagc gcctggttct tcccagcccg ctggcggacg tacatcgcga
     6661 cgtgccatca ggtcatcaat ctgcggtgcg tcgatagtct catatttcat gagagcatct
     6721 ttcatcgcat gcagaatatc catattgtcg gtcagaagct gacgcgcacg attatagtta
     6781 cgctcaatca gtgctttcac ttcctggtcg atgatacgtg cagtttcatc ggacatatgt
     6841 ttcgctttcg ctacgctacg gccgaggaac acttcacctt cttcttccgc gtacagcagt
     6901 ggacccaatt tctcagagaa gccccactga gtcaccatgt tacgcgccag gttggtcgca
     6961 actttaatat cgttggacgc accggtagaa acatgttccg gcccgtagat gatctcttct
     7021 gccagacgac caccgtacag cgtagaaatc tggctttcca gtttctgacg gctggcgctg
     7081 attgcgtcgc cctcaggcaa gaagaaagtc acacccagcg cacgaccgcg tgggataatc
     7141 gtcactttgt gcaccggatc gtgttccggc accaggcgac cgataatcgc atgacccgct
     7201 tcgtggtaag ccgtcgattc tttctgcgct tccgtcatca ccatggagcg acgttccgca
     7261 cccatcatga ttttatcttt cgctttctcg aactcaacca tcgacacaac gcgtttgttg
     7321 ccacgagcag cgaacagtgc cgcttcgttt accaggttcg ccaggtcagc accggagaaa
     7381 ccaggagtac cacgggcaat gattgccgcg tcgatatcgg gtgccaatgg tacgcgacgc
     7441 atgtgaactt tcaggatctg ctcacgaccg cgaacatctg gcaagccaac cacaacctga
     7501 cggtcgaaac ggccaggacg cagcagggcc gggtcaagaa cgtccggacg gttagtcgcg
     7561 gcgataacga tgataccttc gttaccttcg aagccatcca tctcaaccag catctggttc
     7621 agagtctgtt cacgttcatc gtgaccaccg cccagaccag cgccacgctg gcggcctacg
     7681 gcgtcgattt catcgataaa gatgatgcac ggtgccgctt tcttcgcctg ttcgaacatg
     7741 tcacgaacac gggatgcacc cacaccgacg aacatttcta cgaagtcaga accggagata
     7801 gtaaagaacg gaactttcgc ttcgcctgca atcgctttcg ccagcagcgt tttaccggta
     7861 cccggaggac cgaccatcag gacgcctttc gggatcttac cgccgagttt ctggaagcgg
     7921 ctcggctcgc gcagatattc aaccagttca gcaacttctt cttttgcttc gtcgcagccc
     7981 gcaacgtcag caaaggtcgt tttgatctga tcttccgtca gcatgcgcgc tttgctctta
     8041 ccaaacgaca tggcaccttt gccaccgccg ccctgcattt gacgcatgaa gaagatccac
     8101 acaccaatca gcaacagcat cgggaaccag gagatgaaga tagaagccag caggcttggt
     8161 tcttcaggcg gttcaccgac aaccttgacg ttcttggtca acaggttatc cagtaatttc
     8221 ggatcctgaa ccggaatgta agtggtataa cggttactat ctttcttggt aacgttgatt
     8281 tcacgtccgt tgatacgcgc ttcacgaacc tggtcgttat tgacctcttg taggaaggta
     8341 gagtaatcca ccttacggcc attagactcg ctgggcccaa agctctggaa tactgacatc
     8401 agcacaacgg caatgaccag ccagagtatt aggtttttcg ccatgtcact caagggatta
     8461 acctcttatt acaactgtgt taaaaacagc gtcaggatac tctatatcca gcatctttca
     8521 aactttcgtc tgaaatctcc cggttagggt ttacgcccgg tcgctacaat atacacttcc
     8581 cgcgaacgtg cacgagaaga gtccggctta cgaactttga ccttcgtaaa cagggagcga
     8641 atttccctta gatactcatc gaaaccttcg ccctggaaca ccttcactac aaaactgcca
     8701 cctggcgcta atacatcacg acacatttct agcgccagtt ccaccagata catggcacgg
     8761 gggatatcca ccgccggtgt tccgctcatg tttggtgcca tatcggacat gacaacctgg
     8821 actttgctgt cgccaacgcg ctccagcagt gctttcatca ccagttcatc acgaaaatcg
     8881 ccctgaagaa agtctacacc aacgatagga tccataggta aaagatcgca agcgatgatg
     8941 cggcctttgc cgccaatttg ggtgaccaca tattgtgacc aaccacccgg agcagcacca
     9001 aggtcgacaa ccgtcattcc cggtttaaag agtttgtcac tttgctgtat ttcatcaagt
     9061 ttaaaccagg cacgggaacg taaccccttt ttctgtgcct gttgaacata tttatcgcta
     9121 aagtgttcct gaagccagcg gctggagctg gcagaacgct tcttacctgt catttaactt
     9181 tcccatgggg ataactcatc gtaaccaatt gcgtaaattt ttacgcgcct atttggtgat
     9241 atatgggaga tggcggtaga atgacccatt ttcaatccca acgtaagcaa aaaatacgat
     9301 gaatctgagt actaaacaaa aacagcacct gaaaggtctg gcacatccgc tcaagccagt
     9361 tgttctgctt ggcagtaatg gtttgaccga aggggtgctg gccgagattg aacaagcgtt
     9421 agagcaccat gaactcatca aggtgaaaat cgccaccgaa gatcgcgaaa caaaaacctt
     9481 gatcgtggaa gctatcgtgc gcgaaaccgg cgcctgtaat gtacaggtca tcggtaaaac
     9541 gctggtgctt tatcgcccaa ctaaagaacg taaaatctcg ctgccacgct aagattatcc
     9601 taaagttaca cacatttgct gtgtaaaacg aggggttttc cgcaggcagg agagcaaaat
     9661 gccacgctct gttcgttgat aaaaggccgc atagcggcct ttttcctttc tttacaatac
     9721 atcaacatct tgagtattgg gtaattctta caggtattcc accttaatta cttcaaattc
     9781 tacttcgccg cccggcgttt tgatgaccac aacatcatct tcttctttgc cgatcaggcc
     9841 acgagcaata ggcgagttta cagaaatcag gttttgttta aagtcagctt cgtcatcgcc
     9901 aacgatgcga taagtctgtt cttcgtcaga atccagattc agcaccgtta cggtagcacc
     9961 aaaaataacg cgcccattgt taggcatttt ggtgacatca atcacctgcg cgtttgacag
    10021 cttggcttcg atgtctttga tacggccttc gcagaagccc tgctgttcac gagctgcgtg
    10081 gtattcggcg ttttctttca ggtcgccatg ctcacgcgct tccgcgatag cagcaatgat
    10141 ttcaggacgg cgcacagatt tcagaaaatc cagctcttcg cgtaattttt cagcgccgcg
    10201 taaggtcatc ggaatagctt gcatttgtta tacctcttga atattcctga tggggcaagt
    10261 cttcacccac cccggctgtt cggcctgccc ggcataactc ctcaccggga ccagaagcaa
    10321 aaaaataccg acccaggtac aagtcccagg tcagctacaa ttcacatttt gatagtcatt
    10381 ttaccctgaa gttcccgaag ggtcatcgtt tactttatag ggcgttgcgc cgtagtatga
    10441 cggctcgatt ccaggttgtt agcgcgagat tatgcgattt tccagattta tcatcggatt
    10501 gaccagctgt atagcgttca gtgttcaggc cgcaaatgtt gatgagtaca ttactcaact
    10561 ccccgctggt gccaaccttg ccctgatggt gcaaaaagtc ggcgcgtcgg cccccgctat
    10621 tgattaccac agtcagcaga tggcgctgcc tgccagtacc cagaaagtga ttactgcgct
    10681 ggcggcgttg attcaactcg gccccgattt tcgttttacc acgacgcttg aaaccaaagg
    10741 caatgtggaa aacggcgtac ttaagggtga cttagtggcg cgatttggtg ccgatccgac
    10801 gttaaaacgt caggatattc gcaatatggt cgcgactttg aaaaaatctg gcgtcaacca
    10861 aatcgatggc aatgtgttga tagatacctc cattttcgcc agccacgata aagcccccgg
    10921 ctggccatgg aatgacatga cacaatgctt tagcgctccg cctgccgccg ccatagttga
    10981 ccgcaactgt ttctccgtct cgctctacag tgcccaaaaa cccggtgata tggcctttat
    11041 acgcgtggca tcttattacc ccgttacgat gttcagccag gtacgcaccc tcccccgtgg
    11101 ttctgccgaa gcgcaatact gcgaactgga tgtcgtacca ggcgacctga accgctttac
    11161 gctgacggga tgcctgccac aacgttctga gccgctcccg ttggcttttg ccgtgcagga
    11221 tggagccagt tatgccggtg caattctgaa agatgagtta aaacaggcgg gtatcacctg
    11281 gagcggaaca ctgctgcgcc agactcaggt taacgaacct ggaacggtag ttgccagtaa
    11341 acagtcggcc ccgctgcacg atctgcttaa gattatgctg aaaaagtcgg ataacatgat
    11401 cgccgatacg gttttccgca tgataggcca tgcgcgcttc aatgtacctg gtacatggcg
    11461 ggccgggtcg gacgctgtgc gtcagatcct gcgccagcaa gccggtgtcg atattggaaa
    11521 caccattatt gccgatggtt cagggctttc gcggcataac ctgattgccc ccgccaccat
    11581 gatgcaggtg ctgcaataca ttgcccaaca cgacaatgaa cttaacttta tctccatgct
    11641 gccgctggcg ggctatgacg gctctttgca gtaccgtgca ggtctgcatc aggcgggcgt
    11701 ggatggaaaa gtctcagcga aaaccggttc gttgcagggg gtatataacc tggcgggatt
    11761 cattaccaca gcgagcgggc aacgaatggc gtttgtgcaa tatctttctg gctatgcagt
    11821 agaacctgcg gatcagcgta atcgccgtat tccgttagtg cgttttgaaa gccgtttgta
    11881 taaagatatt tatcagaaca attagtcaaa aagaaacccc ggcacatggc cggggcttca
    11941 gattattgac aaagtgcgct ttgtttatgc cggatgcggc gtaaacgcct tatccggcct
    12001 acaaaatcgt gcaaattcaa catattgcaa ttctcttgta ggcctgataa gcgtagcgca
    12061 tcaggctgat ttggcgttta tcatcagtga ttaacgcttg taaatgaact caacgccttc
    12121 ttcgtcgtct tcgtcccagt cgtcatccca gtcttcatca tcctcttcag caatctcttc
    12181 aagctgctgg cgatgataat cgtcccacat gaattcgact ttctctggct gtttcgcttc
    12241 ttcagcctgc acgaccgggt tttcaaggat aaaggtcatc acatcccagc agagatcttt
    12301 cacgcccagt ccgctcgccg cagagatcag ataatattta tcttcccagc ccagcgcttc
    12361 agcaatcgct ttcgctttct cttcagcttc tgccttatcc agcagatcga tcttattgaa
    12421 cactaaccaa cgcggtttcg ccgccagatc ctggctgtat ttttccagct cgctgataat
    12481 aatacgcgcg ttttcaaccg gatcggtgcc gtcaatcgga tcgatatcga tgaggtgcaa
    12541 caggacgcgg caacgttcca ggtgcttcag gaagcggata cccagacctg cgccttccgc
    12601 agcgccttca atcagtcctg gaatatcggc aacaacgaag ctcttttcgt tgtccattcg
    12661 taccacaccc agacttggca ccagagtggt aaacggataa tccgccactt tcggtttagc
    12721 cgccgatacc gcgcgaataa aggtcgattt acccgcgttt ggcatcccca acataccgac
    12781 gtcagccagc agcatcagct ccagcagcag ctcacgctta tcacccggcg taccgttggt
    12841 tttctgccgt ggtgtacggt taacggacga tttgaaacgg gtattgccca gaccgtgcca
    12901 gccgccctta gcaaccagca gacgctgacc gtgtttagtc atatcgccca tggtttcacc
    12961 agtaccctgg tcgattacac gcgtacctac cggcacttta atcgtcacgt ctttaccgcg
    13021 cttaccggta caatcgcggc ttgcgccgtt ctgaccgcgc tctgcacgga aagatttttc
    13081 aaaacgataa tcgataagcg tgttcaggtt ttcgtcggct tccatccata cgtcaccacc
    13141 atcaccaccg tcgccgccat ccgggccgcc tttcggaata tacttttcgc ggcggaagct
    13201 cacgcaacca ttaccgccat cacctgcaac gaccagaatc gatgcttcat caacaaactt
    13261 cattttattc tccataaatc attcgcctgc gcggggttgc gataccaccg ttgtatgctt
    13321 acgtaatccg ccccaaatac gatgaccaat ggcggaatac atcgcgcccg caaccacgac
    13381 aaacgcaccg agataaccta aaaggtttaa catcggtctg gcgaagaaat cgggccaggc
    13441 cagtgataaa agatctgaaa aaaacagcgt aaacagtggg gtgagcgtga tgatcgcgct
    13501 cacctgcgct gcctgccagc gagccatcgc ttccgccagg gcgccatatc ctaccaatgt
    13561 attcagtccg caaaaaatta aacatgcgag ctgccagtgg ctaagctgcg ctatcactcc
    13621 aggctttgcc agagggaaga gcgcaattgt acataaagtg tacagtaaaa acaggatctg
    13681 cggtgaggcc agccgacgca ataaaacctt ttgcgccacg ccataactca cccaaaccgt
    13741 cgccgcaccg accccaaaga taactcccca ggtgtaatcg gtgagctttg taaatatctc
    13801 gaccagactg gtgttaaaaa acatcaccag gccgctcagg agcatcaatg cccctacaac
    13861 ctgagtgctg cgcattttct ctttcaggat aaatacgctg gcaaccatca tgccaactgg
    13921 cgagagttgc ccaatcacct gcgaagcggt cggactcagg tattgcaagg atgagctgaa
    13981 caggatgaag ttcccaaaca gcccggcggt cgccactgcc aacaaaatca accagcgtgg
    14041 cttacgaaac acgcgtaatg gcggcaacct cttcttcacc gcaagaatgg caccaaggcc
    14101 aatactcgcc atcaagaaac ggtaaaacac gattgtcgga ggttccatca cctccagcac
    14161 ctgcttcatt gcgattggca acgcccccca gcaaattgct gtggtgagcg ccaaaagaat
    14221 gccaatgcct gcctgctgct tcataccgta ttccctgcaa agcgcatttg cggattaacg
    14281 tcgcaccatg cgcattttta ccggttatcg aatgtaaaaa gccccgcaac gtgttgcggg
    14341 gctttcatcc gttaccggga cgcgaaaaac ttattcagct tcgatgctga taaatttacg
    14401 gtttttcggg cctttaactt cgaatttcac tttaccgtct gctttagcaa acagagtgtg
    14461 gtcacgaccg caacctacgt tagcgccagc gtggaatttg gtaccacgtt gacgaacgat
    14521 gatgctaccc gccagaacgg attcgccacc gaaacgctta acgcccaggc gtttagcttc
    14581 tgaatcgcga ccgttacgtg tggagccgcc agccttttta tgtgccattt gaaatctctc
    14641 ctcaggtctt aggcgctgat gccagtaatt ttcacatcag tgaaccactg acgatgaccc
    14701 tgctgcttac gatagtgttt acgacgacga aacttaacga ttttaacttt ctcgccacga
    14761 ccgtgagcaa caacttcagc tttgattacg ccgccatcaa cgaaaggaac gccgattttg
    14821 acttcttcac cgtttgcgat catcagcact tcagcgaact caacagtttc gccagttgcg
    14881 atgtccagct tttccaggcg aacggtctga ccttcgctta ctcggtgttg tttaccacca
    14941 ctttggaaaa ccgcgtacat ataaaactcc gcttccgcgc acaccttttc aatgattcag
    15001 agtgcgctat aaatattcac aatagggcgc gaatattacg caaaacgcac gcctttgaca
    15061 agtgctacag tcaatacacg aagaaaaaaa acacaacttg tacggtaacg tttatctgtg
    15121 ccattttttc agtacaatca ccctatattc ctaaccataa accctaagtt gcctttgttc
    15181 acagtaaggt aatcggggcg aaaagcccgg cttttgcgat gaatttagaa aaaatcaatg
    15241 agttaaccgc gcaagatatg gcgggtgtta atgcggcaat ccttgagcag cttaattccg
    15301 acgtccaact gatcaatcag ttaggctatt acatcgtcag cggcggcggt aaacgtattc
    15361 gtccgatgat tgctgtactg gctgcacgag ctgttggcta tgagggaaat gcgcatgtca
    15421 ccattgctgc cctgatcgag tttatccaca cggcgactct gctacacgac gacgttgtgg
    15481 atgaatcaga tatgcgcagg ggtaaagcta ccgccaacgc cgcatttggc aatgccgcca
    15541 gcgtgctggt aggcgatttt atttataccc gcgcgttcca gatgatgacc agcctcggtt
    15601 cactcaaagt gctggaagtc atgtcagaag ctgtaaacgt catcgcagaa ggtgaagttc
    15661 tgcaactgat gaacgttaac gatccggaca tcactgaaga aaactacatg cgcgttatct
    15721 atagcaaaac cgcgcgtctg tttgaggctg ccgcgcagtg ttccgggatt ctggctggct
    15781 gtacgccgga ggaggagaaa ggcctgcagg attatgggcg ctatctcggc actgctttcc
    15841 agttgatcga cgatttactc gattacaatg ccgatggcga acagttaggt aaaaatgtcg
    15901 gcgacgatct gaacgaaggt aaaccgacgc tgccgctgct gcatgcgatg catcatggca
    15961 caccagaaca ggcacagatg atccgtaccg ccatcgaaca gggtaacggt cgccatcttc
    16021 tggaaccggt tctggaagca atgaacgctt gtggatctct tgaatggacg cgtcagcgtg
    16081 ccgaggaaga agcagacaaa gccatcgcag cattacaggt gctcccggac accccttggc
    16141 gagaagcact catcggcctc gcgcacatcg ctgttcaacg cgatcgttaa tcccctccct
    16201 tcatcccgcg cagtgcgcgg gatgattcca gtaaattcca taaaaacact aattcagcgc
    16261 tataacacct gaaaaaaaag ggcgatgaat ctttatatat tctgaatatt cacactcttt
    16321 acaggaactt tttagagcaa taggccatca ggagtatagt gatgcccgac agaagaagtg
    16381 ttctgaatga aagcgactaa cttaaggagt gaggaaagat ggaaagtaat ttcattgact
    16441 ggcatcccgc tgacatcatt gcgggtttgc gcaagaaggg aacgtcaatg gcggcggagt
    16501 ctcgcagaaa tggtttgagt tcctcaacgc tggcaaacgc attatcgcgc ccatggccga
    16561 aaggagagat gattattgcg aaagccctgg gaactgaccc ctgggttatc tggccatcac
    16621 gctaccatga tccgcagacc catgagttta tcgacagaac gcagttgatg cgtagctaca
    16681 ctaaaccgaa aaaatgagtg gtctggcggt agccccgcga acggggctgc cagctctcag
    16741 acgattactc gcctttcaca cgctcaatat ttgcacctaa agcgcgcagt ttgtcttcaa
    16801 tgcgttcgta gccacgatcg atgtgataaa tacgatcaac caccgtcgtc ccttccgcaa
    16861 tacagccagc cagcaccagg cttgctgatg cacgcagatc ggttgccata acctgtgcgc
    16921 cagaaagttt ttcaacaccg tgacaaataa cggtattgct ttcgatttcg gcatgcgcgc
    16981 ccatacggct aagctccggc acatgcataa agcggttttc aaagaccgtt tcggtgatga
    17041 atccggtccc ttctgccacc aggttcaaca gcgtgaactg ggcctgcata tcggtcggga
    17101 atgccggatg cggcgcggta cgtacgttaa cagccttcgg acgtttgcca tgcatatcca
    17161 ggctaatcca gtcttcgccg acttcgatgt ccgctccagc gtcacgcagt ttcgccagca
    17221 cggcgtcgag agtatctggc tgcgcgttac ggcagataat tttgccgcga gaaatcgccg
    17281 ccgccaccag gaaagtaccg gtttcgatac gatccggtag cacgcgatag acaccgccgc
    17341 ctaaacgttc cacaccttcg atgacgatac gatcggtgcc ctgaccgcta attttcgcac
    17401 ccagcgtaat caggaagttc gcggtatcga cgatttccgg ttcacgcgct gcattttcaa
    17461 taatcgtggt accttccgcc agggttgcag cacacatgat ggtcaccgtt gcgccaacgc
    17521 tgactttatc catcacgata tgtgcgcctt tcagacgacc atcgacggaa gctttaacgt
    17581 aaccttcttc cagtttgatg gtcgcgccta attgttcgag gccagaaatg tgtagatcaa
    17641 ccggacgcgc accgatcgta caaccgccag gcagtgaaac ttgcccctga ccaaagcgcg
    17701 ctaccagcgg ccccagtgcc cagatagaag cacgcatggt tttaaccaga tcgtaaggtg
    17761 cgcagaatac attaacgtcg cgggcatcaa tatgcacaga accattacgt tctactttcg
    17821 cacccagctg gcttagcagc ttcattgatg tatcgacgtc tttcagtttt gggacgttct
    17881 ggatctctac cggttcttcc gccagtagag cggcaaaaag gataggcaga gcagcatttt
    17941 tagcgccgga aattgtgact tcgccctgga gcttcgttgg cccctgaaca cgaaatttat
    18001 ccatttagtt tgttctcagt taacaattca tatccgctac cggcgaatcg cccatagctc
    18061 aaaagccgtt cagtttgcga tcgcgcgccc actccgcagg ggtatacgct ttgatcgaca
    18121 cagcatgaat gcggttatcc gcaatatatt ccatcagcgg gccatagacc gtctgctgtt
    18181 ttttaacccg actcatgccg tcaaacaact cacccacggc aataacctga aagtggctgc
    18241 catcgccgga aacgtggact tcctggaggg agagagcgtt catcaacacg ctctgaattt
    18301 cattattttc catgggatct tcaatcatca gttaataaac cagcgaaaca tcttagagca
    18361 aagttgcgct ggcataaata agcaaaaagc ctcgctgata aatcagacaa ggctcgactt
    18421 gcaggcaggt ttgccggaca ggcggttaac gccatatccg gcctgaaaaa atttaacgag
    18481 gcagaacatc agcaggcaaa ttatacaatt tcgcgagggt atacactttg tcgtttaccc
    18541 cctgaagcgt cacgttgttg ccctgctttt tcgccagatc gataagatgg agcagcagtg
    18601 ccagtccacc cgtatccacg cgggacacac ggctaagatc gatgcaggta atccccttca
    18661 ccgcttcctc acgcatttcc caaagcggta gcagaacgtc ctgatccagc tctccggata
    18721 acgccagcgt gtcacccgtc tgcatccagc tcagtgactc gctcattatt ttttctcttc
    18781 cagagtgatt ttctgttgag aaatcgattt cagttgcgca gtcaggccgt cgataccttt
    18841 ggtacgcagc agcgttcccc actcgttttg tttggtggtg atcatactga cgccttcagc
    18901 aatcatgtcg taagcctgcc agtttcccgt ctgggagttt ttacgccact ggaagtccag
    18961 acgcaccggc ggacggccat tcgggtcaat aatggtaacg cgaataggca caatggtttt
    19021 atcgcccagc ggctgttctg gcgcaatctg ataggtttga ccgtgataca tcgccagcgc
    19081 ctgaccgtaa gcctgcttca ggtactcacg gaaagcggca aagtaggctt cacgttgagc
    19141 aggggtcgca ctcttgtaat actggcccag caccagcgca ccggcgtatt tcacctgtac
    19201 gtatggcagc agttcctgat caacaatggt acgcagataa tccgggttgg cccgaatttg
    19261 cggttgctca ttcttcaggc gatcgaacgt tttctgcgcc gcctcgtcca tcagcttata
    19321 aggattggtc tggtctgccg cggttgccgc actcagaggt gcaatcacca gcaaagcgac
    19381 catcattaaa cgtttaaaca tgcgtcggtt ctcctgaaat tatttcgttg tacccacagg
    19441 ttcagtggtt tcattattac ctggcgcagc agctggcgca tcgccactat tcttattgtc
    19501 atcgccttta ctaccgtaaa ggaactgacc aatgagatct tccagcacca tcgcagactt
    19561 agtgtcctga attgtatcgc catccttcag gatagcagtc cccagttccg ggtcttcaaa
    19621 accgacgttt aatgccagat attgttcccc cagcaggccg gaagtacgaa tgctcagcga
    19681 actggtatct ggaatgtggt tataacgttg ttcaatttcc agcgttacgc gcggcagata
    19741 ggttttcggg tccagcgtaa tatccgccac ccgacccaca acaacgccac caatactgac
    19801 cggagagcgc gctttcaggc cgccaatgtt atcgaacgtc gcataaagcg tgtaggtcgg
    19861 ttcagtacgt atggacgtca cgttcgccgc cttcaggcaa acaaacagcg ccgccagcag
    19921 tgctgctaat aaaaagatac ccacccaaat ttcatttttt ttcgtttgca tgaactcaat
    19981 tcccaaacat caatgcggtc agcacaaaat ccagccccag aacagccaga gacgagtgga
    20041 caacggtgcg agtggttgcc cggctaatcc cggcagacgt cgggatggtg tcgtagccgt
    20101 taaacaacga aatccacgtc accgtgatgg cgaacaccac gctcttaatc agacagttgg
    20161 ccagatccat acgccagtcg acggcatttt gcattgccga ccagaagaac ccgctatcaa
    20221 tgcctttcca actgacgccg accagagatc cgccccagat ccccacggcg acgaaaataa
    20281 ccgtcaacag tggtaatgaa ataaccccag cccagaaacg gggagaaata acccgacgca
    20341 gcggatccac cgccatcatc tccatactgg agagttgctc tgtagcgcgc atcaggccga
    20401 tttctgcggt tagcgccgaa ccagcacgcc cggcaaacaa caacgcggca accaccggcc
    20461 ccagttcacg cagtagcgat aacgccacca gcatacccag actggtttcc gcactataag
    20521 tggtcagaac cagataacct tgcagcccca acaccattcc gatgaacacg ccagaaacca
    20581 caataatcag catcgacagg acgccgacat tatagagctg gcgcaccagc agcggcgcat
    20641 gtttgcgaaa ttccggtttg ccgaccagcg cattgaataa cattaacccg gcccgcccga
    20701 acgttctcag ggttttaatc cctttatgtc cgagcgacgc cagcgcattt aacagcatga
    20761 gtggcttaac tccctggtaa aagatcagcg tgataatcgc cggcaggata gcggaacgga
    20821 acaggcccgt cagctatccc gtccagaaac tgacgtacgc gcggatcagg attcgcctgc
    20881 aacgcctggg cactgccatg agcgacaatt tttttgtccg ccaggatcca ggcgtgatcc
    20941 gcaatactta acacttccgg cacatcgtga gaaaccacca cacaagtcac gcccagcgcg
    21001 ctgttcagct cagaaatcag cttcaccagt acgcccatgg tgatgggatc ttgcccaaca
    21061 aaaggttcat caaacatgat gagatccggc tccagcgcaa tcgcacgcgc cagcgctgca
    21121 cgccgcgcca tcccaccgga aagttcagaa ggcattagtt tagccgctcc acgcagcccc
    21181 acggcctcca gcttcatcat caccgtacta tgcaacaatg gcgcgggtag ttgggtatgt
    21241 tcgcgcagtg gataggcgac gttgtcaaat acgttcatat cagtgaacaa cgccccggac
    21301 tgaaataaca tgctcatccg tttgcgcact gtatacaggc gcgaacgaga catcgccgga
    21361 atattctcac catcgaaaag gatctcacca tgatctggtg cgatttgccc gccaatcaga
    21421 cggagtagcg tcgttttacc gatgcccgat ggccccatga tcgccgtgat cttccctcgc
    21481 ggcacggtca gggaaatatt atcgaagatg cagcgattgc cacgcgtaaa actgacatcg
    21541 cgcatatcga ctaaattcgc cacagactgc tccataattc acccttcgtc ttgcgttgat
    21601 tttctaagca tggcgctcaa tttaaccttg aacccaacat atttacagaa tattacccgc
    21661 cgtggttagc gaaagctggc atttgtttta ctttttagcc gcataaagtc aaaattaaga
    21721 atccgttacg gctttctgaa aatcttcagc ggaccgacga gtatacctga agaaaggacg
    21781 ttagatgctt ttagctacgg cactgttaat tgttggttta cttttggtcg tttacagtgc
    21841 cgaccgcctg gtttttgccg cgtctattct ttgccgaacc tttggcatcc cgccgctgat
    21901 catcggcatg acggtggtca gtattggtac atcgttacca gaaatcatcg tctcgcttgc
    21961 cgcgtctctg cacgaacaac gcgatttagc cgttggtaca gccctcggct caaacattat
    22021 caatattttg ctgatcctcg gtctggctgc gctggttcgt ccttttaccg tccattccga
    22081 tgttctgcgt cgtgaattac ccttaatgtt gttggtcagc gtagtggccg gttccgtact
    22141 ctatgacgga caacttagtc gcagcgatgg tatctttctc ctctttctgg ctgtgctatg
    22201 gctgctgttc attgttaaac ttgcacgtca ggctgaacgt caggggactg acagcctgac
    22261 cagagagcag cttgcagagc tgccgcgtga cggcggattg cccgtcgcgt ttttatggct
    22321 cggcattgcg cttatcatca tgccagtggc cacgcggatg gtggttgata acgccacggt
    22381 gctggcgaat tactttgcca tcagcgagtt gacgatgggt ctgacggcaa ttgctatcgg
    22441 aaccagcctg ccggaactgg caaccgcaat agcgggggtt cgcaaaggtg aaaacgacat
    22501 tgctgtcgga aatatcattg gcgcaaacat ttttaatatt gtcatcgtgt tgggtttacc
    22561 cgcgctgata acgccaggag agattgagcc attggcgtac agtcgtgact acagcgtgat
    22621 gttgctggtg agcattattt ttgcgttgct gtgctggcgg cgctccccgc aaccgggccg
    22681 tggtgtaggg gtattattaa ctggcggatt tatcgtatgg ctggcgatgt tgtactggtt
    22741 atcgccaata ctcgttgaat aactggaaac gcattatgtc gcacgtagag ttacaaccgg
    22801 gttttgactt tcagcaagca ggtaaagaag tcctggcgat tgaacgtgaa tgcctggcgg
    22861 agcttgatca atacatcaat cagaatttca cgcttgcctg tgaaaagatg ttctggtgta
    22921 aagggaaagt tgtcgtcatg gggatgggga aatcggggca catcgggcgc aaaatggccg
    22981 caacgtttgc cagcaccggt acaccttcat ttttcgtcca tcctggtgaa gccgcgcatg
    23041 gtgatttagg catggtcacc ccacaggatg tggtgattgc tatctctaac tcaggtgaat
    23101 ccagcgaaat cacggcctta attccagtgc ttaagcgtct tcacgtaccg ttaatctgca
    23161 tcaccggtcg cccggagagc agcatggcgc gcgccgcaga tgtgcatctg tgtgttaaag
    23221 tagcgaaaga agcctgtccg ttagggctgg caccgaccag cagcaccacc gccacgctgg
    23281 ttatgggcga tgccctcgct gtcgcgctgt taaaagcacg cggctttact gctgaagatt
    23341 ttgcgctctc acacccaggc ggcgcactgg gtcgtaaact tctgctgcgc gtaaacgata
    23401 ttatgcatac gggcgatgag atcccgcatg ttaagaaaac ggccagtctg cgtgacgcgt
    23461 tgctggaagt tacccgcaaa aatcttggta tgactgtcat ttgcgatgac aatatgatga
    23521 ttgaaggcat ctttaccgac ggtgatttac gccgtgtctt cgatatgggc gtggatgttc
    23581 gtcagttaag tattgccgat gtgatgacgc cggggggaat acgtgtgcgc cctggcattc
    23641 tggccgttga ggcactgaac ttaatgcagt cccgccatat cacctccgtg atggttgccg
    23701 atggcgacca tttactcgga gtgttacata tgcatgattt actgcgtgca ggcgtagtgt
    23761 aaagattcaa ggataaacaa caatgagcaa agcaggtgcg tcgcttgcga cctgttacgg
    23821 ccctgtcagc gccgacgtta tagcaaaagc agagaacatt cgtctgctga tcctcgatgt
    23881 cgatggcgta ctgtcagatg gcctgattta tatgggcaat aatggcgaag agctgaaagc
    23941 gttcaatgtt cgtgacggtt atggcattcg ttgtgcgctc acctctgata ttgaagtcgc
    24001 tatcattacc gggcgaaagg ctaaactggt agaagatcgt tgtgccacat tggggatcac
    24061 tcacttgtat caggggcagt caaacaaact gatcgccttt agcgatctgc tggaaaaact
    24121 ggcgattgcc ccggaaaatg tggcttatgt cggcgatgat ctcatcgact ggccggtaat
    24181 ggaaaaagtg ggtttaagcg tcgccgtggc cgatgcgcat ccactgttga tcccgcgcgc
    24241 cgattacgtg acgcgcattg ctggcggtcg tggcgcagtg cgcgaagttt gcgacttatt
    24301 actcctggcg cagggcaaac tggatgaagc caaagggcaa tcgatatgag taaagccaga
    24361 cgttgggtta tcattgtgct atcactggcg gttctggtga tgatcggcat tgatatggcc
    24421 gaaaaagacg ataccgccca ggtggtcgtc aacaacaatg atcccaccta taaaagcgag
    24481 catacggaca cgctcgtcta taacccagaa ggggcactaa gctatcgatt gattgctcaa
    24541 cacgttgaat attattccga tcaggccgtt tcgtggttta cgcagccggt acttaccacg
    24601 tttgataagg ataaaatccc gacatggtcc gtaaaagcag ataaagccaa gctgaccaat
    24661 gaccggatgc tctatttata tggacacgtt gaagtcaacg cacttgtgcc aaactctcaa
    24721 cttcgcagaa tcacgacgga taacgcgcag atcaatctgg tgacgcagga tgttacctct
    24781 gaagacctcg tcacgttata cggaacaaca tttaactcca gcggtctgaa aatgcgcggc
    24841 aacttacgca gcaagaacgc cgagctgatt gaaaaggtta gaacatccta tgaaattcaa
    24901 aacaaacaaa ctcagcctta atcttgtgct tgccagctca cttctggccg ccagcattcc
    24961 ggcatttgcc gtaaccggag acactgatca gccgatccac attgaatcgg accagcaatc
    25021 tcttgatatg caaggcaacg tggttacctt taccggtaat gtcatcgtca cccagggcac
    25081 catcaaaatt aatgccgaca aagtggtcgt tacccgtccg ggcggcgaac aaggtaaaga
    25141 agtgattgac ggctacggta aaccggcaac gttctaccag atgcaggaca acggtaaacc
    25201 cgttgaaggt cacgcttccc agatgcacta cgaactggca aaagattttg tcgttctgac
    25261 gggtaatgct tatctgcagc aggtcgatag caacattaag ggcgataaga tcacttacct
    25321 ggtaaaagag cagaaaatgc aggctttcag cgacaaaggc aagcgcgtaa ccaccgttct
    25381 ggtgccgtcg cagctgcagg acaaaaacaa caaaggccag accccggcac agaagaaggg
    25441 taattaattc gttatggcaa cattaactgc aaagaacctt gcaaaagcct ataaaggccg
    25501 tcgcgtggta gaagacgtca gcctgaccgt caactccggg gaaattgtcg gtctgctggg
    25561 gccaaacggt gccggtaaga ccaccacttt ctacatggtt gtaggcattg tgccgcgcga
    25621 tgcgggcaac atcattattg atgatgacga tatcagtctg ctgcctctgc atgcacgcgc
    25681 gcgccgcggt atcggctatc tgccacagga agcctccatt ttccgtcgcc tcagcgttta
    25741 cgataacctg atggcggtac tgcaaattcg tgacgacttg tctgctgaac aacgtgaaga
    25801 ccgcgcgaac gagctgatgg aagagtttca cattgagcac ctgcgtgaca gcatggggca
    25861 gtcactctcc gggggtgaac gtcgccgtgt agaaattgcc cgcgcactgg ctgcgaatcc
    25921 gaaatttatt ctgctcgacg aaccgtttgc cggggttgac ccgatctcgg ttatcgacat
    25981 taaacgcatc attgagcacc tgcgcgacag cggcctgggc gtgctgatca ctgaccacaa
    26041 cgtgcgtgaa acactggcgg tttgtgaacg cgcttatatc gtcagtcagg ggcatttgat
    26101 cgcccacggc acgcctacag aaatcttaca agacgaacac gttaagcgtg tataccttgg
    26161 ggaagacttc agactctgat agggtagaag tttgcgacgt tttagcagga gagtacgatt
    26221 ctgaacatga agcaaggttt gcaactcagg cttagccaac aactggcgat gacgccacag
    26281 ctccaacagg caattcgtct gttgcagttg tcgacgctgg aacttcagca ggagctacag
    26341 caggcgctgg agagtaatcc gctgcttgag caaatcgaca ctcatgaaga aatcgacacc
    26401 cgcgaaacgc aagacagtga aacgctggac accgccgacg cgctcgaaca aaaagagatg
    26461 ccggaagagc tgccgctcga tgccagttgg gacaccattt acaccgctgg tacaccatcc
    26521 ggcaccagcg gtgactacat tgacgatgag ctgccggtct atcagggcga aacgacgcag
    26581 accttgcagg attacctgat gtggcaggtc gagctgacac cgttttccga cactgaccgc
    26641 gctattgcta cctctatcgt cgatgccgtt gatgacatcg gttatctgac tgtcccgctg
    26701 gaagatattc tcgaaagtat gggcgatgaa gagatcgaca tcgacgaggt tgaagccgtc
    26761 cttaagcgga tccaacggtt tgatccggtc ggtgtagcgg caaaagatct gcgtgactgc
    26821 ctgctaatcc aactctccca attcgataaa accacgccgt ggctggaaga ggccagactg
    26881 atcattagcg atcatctcga tctgttagcc aatcacgact tccgcacttt aatgcgcgtc
    26941 acgcgtctga aagaagatgt gctgaaagaa gccgtcaatc tgatccagtc gctcgatccg
    27001 cgccccgggc agtcgatcca gactggcgaa cctgagtatg tcattccaga tgtgctggtg
    27061 cgtaagcata acggtcactg gacggtagaa ctcaacagtg acagcattcc gcgtctgcaa
    27121 atcaaccagc actacgcctc gatgtgcaat aacgcgcgca acgatggtga cagccagttt
    27181 atccgcagca atctgcagga tgccaaatgg ttgatcaaga gtctggaaag ccgtaacgat
    27241 acgctactgc gcgtgagtcg ctgtatcgtt gaacagcagc aagccttctt tgagcaaggt
    27301 gaagaatata tgaaaccgat ggtactggcc gatatcgccc aggctgtcga aatgcatgaa
    27361 tcgacgatat ctcgcgtgac cacgcaaaaa tacctgcata gtccacgagg catttttgaa
    27421 ctgaagtatt tcttttccag tcacatcaat accgagggcg gcggcgaagc ttcctccacg
    27481 gcgattcgtg cgctggtgaa gaaattaatc gcggcggaaa acccagcgaa accgttgagc
    27541 gacagcaagt taacctcttt gctgtcggaa caaggtatca tggtggcacg ccgcactgtt
    27601 gcgaagtacc gagagtcttt atccattccg ccgtcaaacc agcgtaaaca gctcgtttga
    27661 cccaaccgat aaggaagaca ctatgcagct caacattacc ggaaataacg tcgagatcac
    27721 cgaggcactg cgcgaatttg ttacagccaa atttgccaaa cttgagcaat attttgaccg
    27781 aatcaaccag gtctatgttg ttctgaaagt ggagaaagtc acccacacct cagatgcaac
    27841 actgcatgta aacggtggtg aaattcatgc cagcgcggaa ggtcaggaca tgtacgctgc
    27901 cattgatggc ttaattgata agctggcacg ccagttgacc aaacataaag ataaactgaa
    27961 acaacactaa ttgtccgggc aattagcatg tgcatggcgg cctgttgtgc ggcacaacgg
    28021 gccatttgta cggttaatgc tccgagcctg ttccactgtt tgagtgggca ggttcttagg
    28081 tgaaattatg acaaataatg atacaactct acagcttagc agtgttctta acagggaatg
    28141 tacgcgaagc cgcgtccact gtcagagcaa aaaacgcgcc ctggaaatca tcagcgagct
    28201 ggcggcgaaa caacttagcc tgccgcctca ggtggttttt gaagctatcc tgacgcggga
    28261 aaaaatgggc agtaccggta tcggcaatgg tattgccatt ccgcatggca aactggaaga
    28321 agatactctg cgcgccgttg gcgttttcgt tcagctcgaa acgcctatag ctttcgatgc
    28381 catcgacaac caaccggtgg acttactttt tgccctgctg gtgtcggcag accaaactaa
    28441 aacgcacctg catactctgt cgctggtggc gaaacgtctg gcggacaaaa ccatctgccg
    28501 ccgtttacgc gcagcccaga gcgatgaaga gctgtatcaa atcattacgg ataccgaagg
    28561 tactccggat gaagcgtagt tattcggtaa tgtctctttt agacgttgtg aggagaaaca
    28621 gtacatggta ctgatgatcg tcagcggacg ttcaggttca ggtaaatctg tcgccctgcg
    28681 tgcgctggaa gatatgggtt tttactgcgt ggataacctt cccgtagtgt tgttacccga
    28741 tctggctcga accctggccg atcgcgagat ttctgccgcc gtcagcattg atgttcgtaa
    28801 tatgccggag tcaccagaaa tattcgaaca ggcgatgagt aacctgcctg acgctttctc
    28861 accgcaactc ctgttcctgg atgccgaccg taatacctta attcgtcgtt acagtgacac
    28921 gcgccgactg catccgcttt ccagcaaaaa cctgtcgctg gaaagtgcta tcgacaaaga
    28981 aagcgatttg ctggagcctc tgcgttcgcg agcggatctg attgtcgata cctcagaaat
    29041 gtccgttcac gagctggctg aaatgctgcg tacccgtctg ctgggtaaac gtgaacgcga
    29101 actgaccatg gtctttgagt ctttcggctt caaacacggt atccctatcg atgcagatta
    29161 cgtctttgac gtgcgcttct tgccgaaccc gcactgggat ccgaaactgc gtccaatgac
    29221 aggtcttgat aaacctgtcg ccgcgttcct cgaccgccac acagaagtac acaattttat
    29281 ctaccagacg cgaagctatc ttgagctatg gttacctatg ctggaaacca acaaccgtag
    29341 ctacttgacg gtcgccattg gttgtaccgg cgggaagcac cgttcggtgt atattgcaga
    29401 gcaactggca gactacttcc gctcgcgcgg taaaaacgtc cagtcacgcc atcgtacgct
    29461 ggaaaaacgt aaaccatgac cgtcaagcaa actgttgaaa tcacaaacaa gctgggcatg
    29521 catgcccggc ctgcaatgaa gctgtttgaa ttaatgcagg gttttgacgc tgaagtgctg
    29581 ttacgtaatg acgaaggcac cgaggccgaa gccaacagcg ttattgcgct gctgatgttg
    29641 gattctgcca aaggacggca gattgaagtt gaagcgaccg gcccacagga agaggaagca
    29701 ctggccgccg ttatcgccct ctttaactct ggttttgatg aagattaatc ttcatcactt
    29761 tgacatacaa ctcccttcaa actccccccg acaataagaa aatcacgtac tgaaatcgtt
    29821 ctcaatcaac gtcatttgta cattttgtgc gcttttcact ttcagaagaa ccttaagaaa
    29881 accttaagag gcattgttta ggttttgttt aagttaatcg accatactgg agatcgtcag
    29941 aaaatatttc caggagatgg catgattcgc ttatctgaac aaagtcccct gggcaccgga
    30001 agacatcgga aatgttatgc gcatccggaa gatgcccaac gctgtatcaa gattgtctac
    30061 catcgtggcg atggtggcga taaagagatc cgccgtgagt taaagtacta cgcgcatctt
    30121 ggtcgccggt taaaagactg gagtggaata ccgcgctatc acggtaccgt tgagacggat
    30181 tgcggaaccg gttatgtcta cgatgtgatt gcagattttg acggcaaact ctccattacg
    30241 ctgaccgaat ttgctgaaca atgccgttac gaagaagaca ttgcacaact gcgccagcta
    30301 ctgaaacagc tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc gctgaagccg
    30361 cagaatatcc tctgtcaccg catcagtgag tcagaggtta tcccggtcgt ctgcgacaac
    30421 attggtgaaa gcacattgat tccactggcg acctggtcga agtggtgttg tctgcgtaag
    30481 caagagcggt tatggaaacg atttatcgcc caaccggcgc tggctattgc cctgcagaaa
    30541 gatctgcaac ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc ttaatccagc
    30601 tggtgttgtt gcataaacgg ctcaccgcct aactgataca tctgccgtaa aatccacgcc
    30661 tgacggctac gcacgtagcc cgatggcgag gagactttga aacgaagtgg attaggtaat
    30721 acagctgcca gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt gtggaaataa
    30781 cgttgtgccg cagcttcgac gccaaacaca ccgtcgccaa attcggcgat attcaggtaa
    30841 accgtcagga tacgcttttt gctccagact gtttctatcc ccagcgttaa tccagcttcc
    30901 agcccttttc gtacccagct acgcccatcc cataaaaaga gatttttggc cgtctgctga
    30961 gaaatcgttg aagcaccgcg aatacggttt tcattgcgct cgttgtgcgc cagggctttc
    31021 tcaatggaag cgacatcaaa gccccagtgc tcagaaaatt tctgatcttc tgcggcaatc
    31081 accgccagtc ccatccacgg cgagatttga tccatactga cccagtcaga atgtgccacg
    31141 taacgaaaat tgccatgcag ccaggcgctg acctgtcgct cgaccattac cgctgagaag
    31201 ggaacaggcg caacactaaa caacgcgatg cccccgcccc agaaaacggc gaggacaacc
    31261 attaacagca aaagaaaacg gcgaacgaaa ctaaacaccg ttaagcggct tttactcatt
    31321 cagccagaac cagcacgcgg gaaaccagct tatcaatgcc gctcgccgct tctgcaatgt
    31381 tctgcgccag catatatgct ggggtggtga caatcttatt gtcttcatca accacgatat
    31441 catcgacagg acagggcaca tgctccgcgc ccatctcttc cagcacttct gcggtatcga
    31501 tatcagtacc gatggtcaaa cgcagcggga aatcgaaaat tttcggcagc atcgccgggg
    31561 caatacacat aaaaccaagc ggttttccgg cctgatgcat cgcttgtgcc agcgccttta
    31621 attcacggtc aacggtgcat tcgctaccaa gagtggcaaa attgcttaaa ttcttcgccg
    31681 cgccaaaccc ccccggcaca atcaacgcat ccagttcagc ggcatcggcc tgggccagag
    31741 gacggatttc accgcgcgtt attcgtgccg cttcaatcag cacgttgcgc gtttccgtca
    31801 tcgcttcgcc agttaaatgg ttgataacat caacctgctg cttatccggt gcaaagcaga
    31861 ccgcctgcgc accgctgcgt gaaatagcta acaacgtcaa caccgcttca tgaatttcag
    31921 aaccgtcata gacgccgcat ccgctcagaa ttacgccaat tttcttcatt gtgatcatcc
    31981 tttcgcaatt gactgaaaca cattaatttt tttaataaaa atggtacgca tcacacattt
    32041 aactgattca tgtaacaaat catttaagtt ttgctatctt aactgcgtgc ggcctgaaaa
    32101 acagtgctgt gcccttgtaa ctcatcataa taatttacgg cgcagccaag atttccctgg
    32161 tgttggcgca gtattcgcgc accccggtct agccggggtc attttttagt ggcttttgcc
    32221 acccacgctt tcagcacttc tacgtcgtga cgccactctt ctttcatctc ttcaatccat
    32281 tcaccgacgt tatcttccca ggcaggaagg tcaggagact gaatttgctg acccagctgt
    32341 tgcagatggc gtaaccccac tgaccccgcc gcacctttaa ttttatgtcc ttcctcaaca
    32401 atgccttttt tatcctgcgc cgtcagattc gactccagca cgctgacata gcccggcatc
    32461 atcttctcaa acaccgctaa cccgtcggtg atcagcttcg gtcctacaag ttcgagatac
    32521 tgttccagca tgggaatatc gagcaatgct tctgatttac tgttctcttc tgtcgtcacc
    32581 gtactctcct catcatcctg ggtatcccag aatttcttga tcatcgcggt tagcgccgga
    32641 acagaaagcg gcttactcag cacatcatcc attccagcat tgaggtactc ttgtttgtct
    32701 ttcagcacgt tagcggttaa ggccaccagc ggcggtaaat cctctcgcgg ataacgcttc
    32761 gtcagttcac gagagatatc cagcccggtc atatctggca actgaatatc cagtaacacc
    32821 aggtcgtatt cgcccggttt aaacatctct agcgccgcct tgccggtcat ggcgacatca
    32881 acgctgttac ctaatttttc cagcacagaa cgcgcaacaa tcacgttcag ttcaatgtct
    32941 tccaccagca gcacattcag cgccggtaaa ggcatatcgt cttcatcaaa cgcatcatcg
    33001 acctcttctg ctaccgacgg tgcgtggatc gtcaacgtaa aggttgaacc tttgccctgt
    33061 tcgctggtaa ccgtaatatc gccgcccata tttttcgcca gacgacgaga aacggccaga
    33121 ccaataccgg tgccggtggc aggtttaccg ccatgactgt ctttcacctg gtaatacatg
    33181 gcgaaaattt tatccagctc atcctgcgga atgccgatac cagagtcttc cacttcaaaa
    33241 tgcagcatat cgccttcatc gtagcgcacg cgcacggtaa cctggccttg ctgggtgaat
    33301 ttgacggcgt tactgatgag gttccacagg atctgccgta aacgcgtccc gtcggtaatg
    33361 acctgatgcg gtaatggcag cgtcggctcc aggttaaagc gcaatccttt ttgttgcgcc
    33421 tgcaatgcgg agagattttc cagatcggca aggaagctgg tgaaatcaac cggttgatta
    33481 tcaagctgga ccttgcgccg ttccatctta tccatgtcga taatatcgtt aaagatattc
    33541 cccagcgtga cggccgaaac atggatggtc ttgagatatt tttcctgctc ggcggtgagt
    33601 tcggtatcca gcagaatgcg gctcagaccg acgataccgt tcagcggtgt acgcaattcg
    33661 tgactgatgg tggagataaa cgtcgttttg tcgcggctgg cccgttcaag cgcatcctga
    33721 taccgcttac gctcggtaat gtctcgacca aagcccatca aaccgtgacg tttacccacg
    33781 cggtcgtagt acggcacttt acggatttca aagcaggctt tgcgcccgtc cgggtaatcc
    33841 agccactgtt cataggtcag tgacacatta tgacggaaca ctttttcatc ggtttcaatg
    33901 acttttgcgg cggcttccgg tgagtaaaca tcaacaggtt tcaggtgaac cagttgtttt
    33961 tcgctttttc cggtcagcag ctccatcgcg cggttacagc cggaaaactc tttatcttcg
    34021 ttacgataaa aaaccaggtc gggtgaagca tcaaggaagg aacgtaagaa tgaggattgc
    34081 tgctcgagct gaatttgtgt ctcttcgcgc tctttgattt caattttcag ttggccgaag
    34141 gtttcctgca gttctgcttc cgctttttca cgaacggcga tttcctgatt tagctgggca
    34201 atattatctt ttaactgaac gttgaggctc aaatcgcgct cgcgcatctc ctccagtttt
    34261 tgcaccagcc gtgacagacg ttgtcgtgac tcctccagtt gctcgacgac caccgataga
    34321 aagtagaccg cccacggcgt aatcaacaaa ccaaagaaga tagaacgaat aacatcaatg
    34381 ctttcgacct gaccatgcag caccatggtt accgccattt gtaccacaat ggcaagaacg
    34441 acgagggcca gcgccagcaa cattgagaag cgcaccagac ctaacttcat catcaggtca
    34501 acataatact gcgccagcag acgaatttgc ttcattaggg aattccttca cgacaacctg
    34561 ccccaataat acccaattct gacagttacg ttataaattg tgtgagaaat gcggaattcc
    34621 cctcaacgac ctactccgtt ggaggtagcc acggacgtcc cagcgccgat ccctgtacgc
    34681 catgttcatt cagatagcga tccagctcga ccattcccgt ccatcgattt tcgcaccaca
    34741 gaggagcaag cagcgtcggg cgacgggcgc tggcggaaat gcggtggtag atcacttccg
    34801 gtggcgtatg gcgaatcatt tctccggcag tgagcgtgta atcctccagt tcaataccgt
    34861 tcaaacgccc cgcttcccag gctttcgcca taatgctgcc tttcacaata tgcaacggat
    34921 gtagctttat accgtcaaca ccggtttcca ctacccgctc cagcgtttgc agacattcag
    34981 ccagcccttc gcctggcaga ccgacaatta aatgcgagca caccttcagc ccgcgctggc
    35041 gtgccagctg cgtcgtgcgc tgataacagg cgaaatcatg accgcgatta atgcgatgta
    35101 gcgttttgtc gtgcacggtt tgcagcccca gctccagcca cacttcgtag ccctggtctt
    35161 tatattcgca aagcagatcc agcaccgcat ccggcacgca gtccgggcgc gtgccgacac
    35221 acaaaccgac aatattggcc tggctcaccg cctgttgata catcgaacgc agcacctgaa
    35281 cttcggcaaa ggtgctggta tacgcctgga aataggcgag gtaacgtttc gcgcgattaa
    35341 ttaaattcgc ctgatgcgcc agctgttcgg caatggaacg atgctgctgc gcttcgtcgg
    35401 caaacgaggc aacattacag aaggtgcagc cgccgcgccc gatggtgccg tcgcgattcg
    35461 ggcaactgaa accgccgtgc agcgtcagct tatgcacctt ttgcccataa cgacgggtga
    35521 gatcaccacc aaacatattg actaatttct gtaactgcat aatctgatag accgcgcctt
    35581 gaaaagaggc caaagcctgc catttttagc tcaattcggc gatgacctgg atcaatcgtc
    35641 ccggcccgct tttatcaact gcataatcaa tcaaaattac cgaaatttca tgcataatca
    35701 cataaatcac ttttgcttat cttgtgtcag atttttttat ctccttatgg attttaggca
    35761 aaaacagtag catgaaacgt cattaccaat taatgcagta taaaatgctg gttttctcgt
    35821 caattcaagg caggataagg gttaacacgc ctttatgaca gtcaggaatt gactgtttct
    35881 ctaacgactt cccttttagc cttaaagata aaatctattt taatttcagc catttaataa
    35941 agaattttgc gctaaagcac atttctgtac caataagctt gccatttgac ctgtatcagc
    36001 tttcccgata agttggaaat ccgctggaag ctttctggat gagcagcctg ctcatcatat
    36061 ttatgcagta attgagatcc cctcttcacc gtattaaccg atgcgaaaag gacaacaagg
    36121 gggcgaatgc gaggcgcgcg tatgacacgc aacccccgtc gccacgctct ttctgtaccc
    36181 gtgcgcagcg gttcggtagt ggggttcccg cagagcctgg gggaggttca cgatatgttg
    36241 tacgataaat cccttgagag ggataactgt ggtttcggcc tgatcgccca catagaaggc
    36301 gaacctagcc acaaggtagt gcgtactgca atacacgcac tggcccgcat gcagcaccgt
    36361 ggcgcgattc tcgccgatgg taaaaccggt gacggttgcg gcttgctgtt acaaaaaccg
    36421 gatcgctttt ttcgcatcgt tgcgcaggag cgcggctggc gtttagcgaa aaactacgcc
    36481 gtcgggatgc tattcctgaa taaagatcct gaactcgccg ctgccgcacg ccgcatcgtt
    36541 gaagaagaac tgcaacgcga aaccttgtcg attgtgggct ggcgtgatgt ccccactaac
    36601 gaaggcgtac tgggtgaaat cgccctctcc tctctgcccc gcattgagca aatttttgtg
    36661 aacgccccgg caggctggcg tccgcgcgat atggagcgcc gtctgtttat cgcccgccgc
    36721 cgcattgaaa agcgtctcga agccgacaaa gacttctacg tctgtagcct atcgaatctg
    36781 gtgaacatct ataaaggtct gtgtatgccg gcggatctgc cgcgctttta tctggatctt
    36841 gcggacctgc gtctggaatc ggccatttgc ctgttccacc agcgcttctc cactaacacc
    36901 gtaccgcgct ggccgctggc gcaaccgttc cgctatctgg cgcataacgg tgaaatcaac
    36961 accatcactg gtaaccgcca atgggcgcgt gcgcgtacct ataaattcca gacgccgctt
    37021 atccctgatc tgcacgacgc cgcaccgttc gtcaacgaaa ccggctctga ctccagttcg
    37081 atggataaca tgctggaact cctgctggca ggcgggatgg atatcatccg cgccatgcgt
    37141 ctgttagtgc cacccgcctg gcagaacaac ccggatatgg acccggaact gcgtgccttc
    37201 tttgacttta actccatgca tatggagccg tgggatggcc cggcgggcat cgtgatgtcc
    37261 gacggtcgtt ttgccgcctg taacctcgac cgtaacggtc tgcgtccggc gcgctacgtc
    37321 atcaccaaag ataagctcat cacctgcgcc tctgaagtcg gtatctggga ttaccagcct
    37381 gacgaagtgg tcgaaaaagg ccgcgtcggg ccaggcgaac tgatggttat cgacacccgc
    37441 agtgggcata ttctgcactc ggcagaaacc gatgacgatc tgaaaagccg ccatccatat
    37501 aaagagtgga tggagaaaaa cgtccgccga ctggtaccgt ttgaagatct gcccgatgaa
    37561 gaagtgggta gccgcgaact ggacgacgac acgcttgcca gctaccagaa acagtttaac
    37621 tacagcgcgg aagagctgga ctccgtaatt cgcgtactgg gcgaaaacgg tcaggaagcg
    37681 gtcggttcga tgggcgatga taccccattc gccgtgctct ccagtcagcc gcgcattatt
    37741 tacgactact tccgccagca gtttgcccag gtgactaacc cgccaatcga cccgctgcgt
    37801 gaagcgcatg ttatgtcgct cgccaccagt atcggtcgtg aaatgaacgt cttttgcgaa
    37861 gcagagggcc aggcgcaccg tttaagcttt aaatcgccga ttctgctcta ctccgatttc
    37921 aaacagctca cgacgatgaa agaggagcac taccgcgcag atacgctgga tatcaccttt
    37981 gacgtcacta aaaccacgct cgaagcgaca gtcaaagagc tgtgcgacaa agccgaaaaa
    38041 atggtacgta gcggcaccgt gctgctggtg ctctccgacc ggaatatcgc taaagatcgc
    38101 ctgccggttc cagccccgat ggcggttggc gcgatccaga cccgtctggt cgatcaaagc
    38161 ctgcgttgcg atgccaacat catcgtcgaa accgccagcg cccgcgatcc gcaccacttc
    38221 gccgtgttgc tgggcttcgg cgcgacggct atttatccat accttgccta tgaaacgctg
    38281 ggccgcctgg tagacaccca tgcgattgcc aaagattatc gtaccgtgat gctcaactac
    38341 cgtaacggca tcaacaaagg cttgtacaaa atcatgtcca aaatgggcat ctccaccatc
    38401 gcctcttacc gctgctcgaa actgtttgaa gcggtcggtc tacacgatga tgtagtgggc
    38461 ctgtgcttcc agggggcggt cagccgcatt ggtggggcaa gctttgaaga cttccagcag
    38521 gatctgctga atctgtcgaa acgtgcctgg ctggcgcgta agcccatcag ccagggcggc
    38581 ctgctgaaat acgtccacgg cggcgaatac cacgcctaca acccggacgt ggtgcgcacg
    38641 ctgcaacaag cggtacaaag cggcgagtac agcgactatc aggaatacgc gaagctggtt
    38701 aatgagcgtc cggcaaccac gctgcgcgat ctgctggcaa ttacgccggg tgaaaacgcg
    38761 gtcaacattg ctgatgttga accggcaagc gaactgttta aacgcttcga taccgctgcg
    38821 atgtctatcg gcgcgttaag cccggaagcc cacgaggcgc tggcggaagc gatgaacagc
    38881 atcggcggta actcaaactc cggtgaaggc ggtgaagatc cggcgcgcta cggcaccaac
    38941 aaagtgtcgc gcatcaagca ggtggcttcc ggtcgctttg gggttactcc ggcgtatctg
    39001 gtcaatgccg acgtcattca gattaaagtc gcccagggcg cgaagccggg cgaaggcggt
    39061 cagttgccgg gtgataaagt cactccttac atcgccaaac tgcgctattc ggtgcccgga
    39121 gtgacgctga tctccccgcc gccgcaccac gatatctact ctatcgagga cttagcgcag
    39181 ctcattttcg acctcaagca ggttaacccg aaagcgatga tctccgtgaa gctggtttcc
    39241 gaaccgggcg taggcaccat cgcgactggc gtggcaaaag cttatgccga tttaatcacc
    39301 atcgcaggct atgacggtgg caccggcgca agcccgctgt cgtcagtgaa atacgcaggt
    39361 tgcccgtggg agctggggct tgttgaaacc cagcaggcgc tggttgccaa tggtctgcgt
    39421 cataagatcc gtttgcaggt cgatggcggc ctgaaaaccg gtgttgatat catcaaagcg
    39481 gcgattctcg gcgcagaaag cttcggcttc ggcactggcc caatggtcgc gctcggctgt
    39541 aaatatctgc gtatttgcca cctgaacaac tgcgcaacgg gtgtggcaac tcaggatgac
    39601 aaactgcgta agaatcacta tcacggcctg ccgttcaagg tgacgaatta ctttgagttt
    39661 atcgcccgtg aaacccgcga gctgatggca cagctggggg taacgcggct ggtggatctg
    39721 attggtcgca ccgacctgct gaaagagctg gacggtttca ccgccaaaca gcagaaactg
    39781 gcgctgtcga agctgctgga gaccgccgaa ccgcatccag gtaaggcact ctactgcacc
    39841 gaaaacaacc cgccgtttga taacggcctg ctgaacgcgc agttgctgca acaggcgaaa
    39901 ccgtttgtcg atgaacgcca gagcaaaacc ttctggttcg atatccgcaa taccgatcgc
    39961 tccgtgggcg cgtcgctttc aggctatatc gcccagacgc acggcgatca ggggctggca
    40021 gccgatccta tcaaagcgta cttcaacggc accgcaggcc agagtttcgg tgtatggaac
    40081 gcgggcggcg tggaactgta cctgaccggg gatgccaacg actatgtcgg taaaggcatg
    40141 gcgggcggct taatcgccat tcgtcctccg gttggttccg ccttccgcag ccatgaagca
    40201 agtattatcg gcaacacctg cctgtatggc gcgaccggtg gtcgtctgta tgccgcaggc
    40261 cgcgcgggtg aacgtttcgg cgtgcggaac tccggtgcta tcaccgtggt agaaggcatt
    40321 ggcgacaacg gctgtgaata tatgacgggc ggtattgtct gtattctggg taaaaccggc
    40381 gttaacttcg gtgcgggcat gaccggcggc ttcgcttacg ttctcgatga aagcggtgat
    40441 ttccgcaaac gcgttaaccc ggaactggtc gaggtcttaa gcgttgacga tctggcgatc
    40501 catgaagagc atctgcgcgg tcttatcacc gagcatgttc agcatactgg ctctcagcgc
    40561 ggtgaagaga ttctggcgaa ctggtcaacc ttcgccacta aatttgcgct ggttaaaccg
    40621 aagtccagtg atgtaaaagc actgctgggt catcgtagtc gtagcgcagc agagttgcgc
    40681 gtgcaggcgc agtaaggggt agcaacaatg agtcagaatg tttatcaatt tatcgacctg
    40741 cagcgcgttg atccgccaaa gaaaccgctg aagatccgca aaattgagtt tgttgaaatt
    40801 tacgagccgt tttccgaagg ccaggccaaa gcgcaggctg accgctgcct gtcgtgcggc
    40861 aacccatact gcgagtggaa atgtccggta cacaactaca tcccgaactg gctgaagctc
    40921 gccaacgagg ggcgtatttt tgaagcggcg gaactgtcgc accagaccaa caccctgccg
    40981 gaagtttgcg gacgagtctg cccgcaagac cgtctgtgcg aaggttcctg cactctgaac
    41041 gatgagtttg gcgcggtgac catcggcaac attgagcgtt atatcaatga taaagcgttc
    41101 gaaatgggct ggcgtccgga tatgtccggc gtgaagcaaa ccggtaaaaa agtggcgatt
    41161 atcggcgcag gcccggcagg tctggcgtgt gcggatgtcc tgacgcgtaa cggcgtaaaa
    41221 gccgttgtct tcgaccgtca tccagaaatt ggcgggctgc tgaccttcgg tattccggcc
    41281 ttcaagctgg aaaaagaggt aatgacgcgt cgccgtgaaa tcttcaccgg catgggtatt
    41341 gaattcaaac tcaataccga agtgggccgc gacgtgcagc tggacgatct gctgagtgat
    41401 tacgatgccg tcttccttgg cgtcgggact tatcagtcaa tgcgcggcgg gctggaaaac
    41461 gaagacgccg atggcgtgta cgcagcgctg ccgttcctta tcgccaacac caaacagtta
    41521 atgggctttg gtgaaacccg cgacgaaccg ttcgtcagca tggaaggcaa acgcgtggtg
    41581 gtccttggcg gtggcgacac tgcgatggac tgcgtgcgta cgtccgtgcg ccagggagcg
    41641 aagcacgtta cctgtgccta tcgtcgtgat gaagagaaca tgccgggttc ccgccgcgaa
    41701 gtgaaaaacg cgcgggaaga aggcgtagag ttcaaattca acgtccagcc actggggatt
    41761 gaagtgaacg gtaacggcaa agtcagcggc gtaaaaatgg tacgtaccga aatgggcgaa
    41821 ccggatgcca aaggccgtcg ccgcgcggag atcgttgcag gttccgaaca tatcgttccg
    41881 gcagatgcag tgatcatggc gtttggtttc cgtccacaca acatggaatg gctggcaaaa
    41941 cacagcgtcg agctggattc acaaggccgc atcatcgccc cggaaggcag cgacaacgcc
    42001 ttccagacca gcaacccgaa aatctttgct ggcggcgata tcgtccgtgg ttccgatctg
    42061 gtggtaaccg ctattgccga aggtcgtaag gcggcagacg gtattatgaa ctggctggaa
    42121 gtttaagctc tgtcccagcg ccgcctcctc agaaaagagt cggcgctgaa attctccttt
    42181 ctttctggaa agcgcctcgc ataatctgaa gaaaaccaat gtaatacgct catctggatt
    42241 gttaccggta gatgcccggc gtatagctga ttccattatt caacgcagcg ccatataaag
    42301 ggacagataa tggaatcgct ctcagagaga acctcaacag gctaccagca aatccacgac
    42361 ggtattattc acctggtcga tagcgcccgg acggaaacgg tacgtagcgt taacgcgctg
    42421 atgaccgcga catactggga aattggccga cgaattgtcg aatttgaaca aggtggcgag
    42481 gcaagggctg cgtatggtgc gcagctaatc aagcgactat caaaggattt aagtctaagg
    42541 tataagcgtg gtttctctgc aaaaaactta cgccaaatga ggctttttta cctctttttt
    42601 caacatgttg aaattcgcca gacagtgtct ggcgaattaa caccattggg aattccgcag
    42661 acaccatctg cagaattccc atcggcaaag atttggcaga cgctgtctgc caaattgaga
    42721 ggctgtttga tatttccaca gcttttgtat tgcatctcct attttcggtt tgtatattca
    42781 tgcaaataaa cgaaggaaag ttgccgtgat ttacattgaa cagcttgaat taatccataa
    42841 aagtggtgat gtgttgtatc cagtaaaaat aaccagaaaa tcatcaggaa aaactgcctt
    42901 ccaccttgtt ccattcggct taaataagac tcacgacctt ctagaggtgg aagatgcttc
    42961 agaagcaatt cggctggtga ttgatgaacg tcattccatt cgctgcagta cacttacagc
    43021 aactattacg aataaaaagg gcaagcgaat taagcggaca ggtatataca gtatcaaggg
    43081 tgttaacata aaagaataca acgtcaggta atggcacaga gcggtccgtc agttcaggtt
    43141 tggctctgtg ccaaagatgg gtcaggtcac acctgatcta ataaatttaa gccattaaat
    43201 cagccttaac cttcaccacc acctttttaa tctcacccgg ctcgccaaca acgcatcccg
    43261 gcttatgcgg ctctcctagc ataaacacgg cgaacattcc cggtttcagg atgatggctt
    43321 gctcgttttc aatggtgctg caaagctggt aatcatcctc atggtggaac tcttcacact
    43381 gacgcgcagt gcctgccatg ccaaacagaa tccgttcttc accgtttaat aacagctgga
    43441 tatcaatgta ttgctcgtgc agctccgctt ttttctcaac gggcgattga gtgttaaacg
    43501 tcatgacatt cataaaaata ttgtcgccct gtaattcgta acgacccggc gctttttctt
    43561 gcggtctggc agctaatgcc agcgttaacg cgtcctgtaa cgcaggatgt aacccagcag
    43621 acggtaatga ccgtacttca cccatcatca taatttttct ccctgggcca acagcgctgc
    43681 cccaagtaaa cctgcatcat ggcggtagtg cgccgccagt aaatcaacat gaaataccgc
    43741 tggctcctgc gccagatacg tttccaccaa cgccagatac ccttctgcca gaccaacgct
    43801 gccaccgacc accacgcact ggcaatcagt tgtggcttta atatcagcga tcagccttgc
    43861 cagcacatgt gcggagcggt gaatcagttg ctgcgcctgc tcgtcgccct gcccggcgcg
    43921 tgtgaaaata gttctcgcat ccgcgcccat gagttcacct tgcgctgccg ctgcaatacc
    43981 gcgaccagaa gcaatcgctt ccacgcaacc tgtgcgtcca cagccgcaga ctgggccgtg
    44041 tggatcggca agcgtatgcc cgatatgccc cgccagaccg ccagggccgg taagcagttt
    44101 accgccgctc actacaccgc cgccgacgcc agtggaaacg gtgataaaca ccatctcggt
    44161 tacatcgcct tccagcgcct gatactctgc ccatgctgcg gcctgcgcgt cgttaatggc
    44221 aatggtcggc aaattggtaa gttgttccag cgttttgact aacggaaagt gtagcaatcc
    44281 accgagatta tgcggattca gcgccagcaa actgccgtcg cgaataattc cggttgaagc
    44341 gatggcaacc cgctgcgcat gagcttgcaa cggagagact aatgcggata aggcatcacg
    44401 caaggcttct ggtgtctggc tggctggcgt aggaagttca cgacgatcgc ggatctgccc
    44461 gtcagcgcca atcagcgcgg cggcaagttt agtaccgccg atatcaatcg ccagtgtggt
    44521 catagcaccg cctttttcat cgctgtgttg taccactgac aaatgtgttc aagacgcgta
    44581 atggcagaac cgaccgtcac cgcccacgcg ccgtggcgca tcgcatctgc cgcctgagca
    44641 ggcgtgttgt aacgcccttc ggcaatcacc cgacatccgg cttcgctcaa cgttttcacc
    44701 agcgccagat ccggctcttc tggcgtttca ggcgtggtat agccagaaag cgtagtgcca
    44761 ataatttcgg ctcccagctt ttggcatgcc aggccgtctt ccggcgttga gcagtcggtc
    44821 atcgccagta aaccgtgatg atgtatacgt gccagcagcg tttcaacagg caccggacgc
    44881 gggcggtcgg tgccgtcaat ggcgataatg tccgcgcccg cctgcgccag cgcatcgaca
    44941 tcttcaatat aggccgtgat gcgtaccgga gaatcctcca gatcgcgttt cacaattcca
    45001 ataatcggca cgctcaccac cgcacgcgtg gcttgcagat ttgccacacc ttcgatgcga
    45061 atggcaaccg cgcccgcctg ctctgccgct aatgccatgg ctgcgacgat ttcgggttta
    45121 tcgagcgggc tgtcaggaat cggctggcag gagacaatca ggccaccgtt agcagcgatt
    45181 ttttgatcca gttgtgcaag taacgacata catcttccct tagcgaaagg cccggtacat
    45241 agaccgggca acaggattaa cttttggttt tgactaaatc gtttttggcg ctgccaaatg
    45301 gcacggcacc gctaaatggt ttgccgtcga tagcgtcatg agtacgcaat gcttccgggc
    45361 gcagccaacg ctgaacgcgg gaaggcatat ccagaccaat cagcaggatc accacaaatg
    45421 tcaggctgaa cgagagcgat gccagcgcag tacccagatc cagacgttga gcgatcaacg
    45481 cgccgatgat tggggccagt gcgccaccca atgcgccaac gttgtaggta aagcccaggc
    45541 ccgctgcacg ctggtcggta tcgaaataac cgccaatcag tttcggtaag atcccggcga
    45601 tcccttgtcc aagcatttgc tggaagaaca gtaacagacc gagcacccag acgtttgcgc
    45661 cgccaatcgc aaataccgga ataatcagca gctgcgaggc cagcaggcta caaacgtacg
    45721 ctttgcgggt tcccagccag tcaccgagga agcctcctac gcagcatccc acggccgcgc
    45781 caaagccact aaagaacagc acattggcga cagtatgcgg gttataagcc agatcggttt
    45841 tcagatacgt tggcagcagc gcctgaatcg gccatgagta gaggaaagca aacaacacga
    45901 ccaccatcag cattacgccc gttggccagc gtttgcctgt actctgcacc ataaagctga
    45961 taaagattgc ggcgcataac agcccaagaa cagcgacgat cgcggcattt tgcaggttac
    46021 cggcgaagca gaaccacagc gcagtagccg ccgccagtgt cattacgata ttggcaatgc
    46081 gatgttcacc acggtagaga atatccacca ttgtgcgtac tggtgcttta cctgcgtgtt
    46141 tctctttcca gtcttccgct tccgggatgt ttttacgcag ccagagagca aagatgattg
    46201 gcaaaatgcc gataaagaac agcgcacgcc aaccccagac cggaaccacc aggctataga
    46261 cctgagcggc aacgacggcc cccacagaga agcctgaaat caaaaaacca ctggctttgt
    46321 tacgcagatg ttttggccag ctttcaatga cataggtggc gctggaaccg tattcacccg
    46381 ccatccccat gccgatgacc agacgagcga taaacatggt gatgtagcct ggcgcaaagc
    46441 cgcaggccag cgtcccggcc gagaagagaa cgatgctggt gaccattgcc agacgacgcc
    46501 cgtagcggtc acccatagcg ccgagcatca ggccgccgaa ccagcgagag ataaaggctg
    46561 cggagatcag acttgccgcc tgcaccgtcg tcagcccgaa ttcaccctgt acttcggtga
    46621 gtaccagggc gattaaaacg aaatcaaaac cgtcaagcag atatcccaac caggcagcgg
    46681 aaaatgcgcg ccattgtgca cggttgagat ggcgatacca cgggatattc tgggttgtag
    46741 tactcattgt gagtctcccg cggtgggcga tgcccacacg ctttggtatg aaaattgtag
    46801 ggtacagatg cgtttatttc ccctcacccg gtaggggcga gcgaggggaa aacaactcac
    46861 ccgcgctctt gcatcaactg ctgggccagc gccttcagtt ctggcagata tttttcatct
    46921 accggtccaa acggtttgcg gcacagcggc acagaaacga catccatata atgcaggaca
    46981 gttttcaggc cgcggaatac gccagttttg atcagtaaat caatgacttt gttgcactca
    47041 gtttgcagtt tctgcgcggt ctggatatcg ccttctttca gcgccttcac gatcccctga
    47101 tatcgccagc ccatgatgtt gtaagtactg ccgataccac catcagcgcc tgccagcaag
    47161 ccggaagcga agatttcgtc gtaaccgtta tagagcacca gatcaggatg ttcacgacgg
    47221 atctgttcca tctgatagag atcgccagaa gtctgtttca gcgcaccgac gccaggcaac
    47281 gtaaccaggg tgttgatctg atccagggtc agttttaccc cactcagggc tggaatgttg
    47341 tacacaacca tcggcaaacc atccgctgaa tcaataattg cccgatagtg atcgcagtgt
    47401 tcttcaaagc tgaaaggata gtagaacggc gtgacggcgg agacggcatc gaagccataa
    47461 cgtttagccg atgccgcaag ttgttggctt tcggcggtgc tgacgcaacc gacgtgggcg
    47521 atgagtttaa tcttaccttt cgcctcttcg gcgacgattt ccagtacctg ttcacgctcg
    47581 gaaaggcttt gtacaaaggc ctcgccggtc gaaccaccca cgtataaacc gtcgatgccc
    47641 tgctgaatat tgaactgaac caggcgacgc agactcgctt tatccagtgc ttgttgttgg
    47701 tcaaaaggag tcaggagtgc agccattacg ccacgtaaat tcgttgccat aaatacctct
    47761 gaagtgatac ttgtctgata aacgatatac ctttatacct gttataccag atcaattaag
    47821 caacacccca tacagaaagc ttataatgcg atctgcttca ctaaagtggc attatttctt
    47881 tttgttggtg gtctgaccga aagcgtgcca ggtagcagag acgctgttga gatgcgattg
    47941 caacgcacga tcggcttcgt caggatcatg acggcggatc gcatcaacga tcgcaatatg
    48001 ctgttgatag ctaacgttgt tatgctcgtg cagagcctga tcagccacgg ttggacgtgc
    48061 ggcgatcagc cagtcgagca gggcaacgtg gatcgccatg aagattgggt taccagggat
    48121 ctccgccagc acgcggtgga aatcaacgtc tgaacgaatg aatgccgcgt tgttatccag
    48181 cgactgactg ttgatttcca gtgcttttgc cagcaaatcg atttgctcat cggtggcatg
    48241 ttcagccgca tagcgcacca gactggattc aaagaacaga cgtaattgtt cgaaatgggc
    48301 aatcccaccg ggatgagaaa ggaaatcttt cgccatgccg gaaaactcac cgatgatagt
    48361 gtccgcagaa ggacgcgaga cgcgagcgcg ttcgccgttg tttatttgca ccagaccttt
    48421 gcgttttaac gctgccagcg cttcgcgcac agaaggacgc ccgacgttaa agaacgccat
    48481 cagttcgcgt tcagacggta attgttcacc ttcgccaaat tcacgacggc ggatcatctg
    48541 ttccagctct tcttccacca tttcggagag ttttttacgc gccagcgggc ggctacgcaa
    48601 gttgcgacca attgcaggtg aagaatcttc ggtttgcgaa tcaaatgcgt tcataaggcc
    48661 cattctgtaa ggtcagtgtg attaacatca tcagtgacat cctatcacag gattgaaagt
    48721 aggggaaaat ggcaggtttt tctctttgtg cctcatcatt accataatta acggaataat
    48781 taactattgc gaaaaattaa tgtaacgcag ataaacacat cccgattgaa ttattcataa
    48841 gactattcac aagcattatg aatattatga atgtgttctt acaaaataat cataagcgca
    48901 tattttttaa tgaaaaatca catcacctac aattaaaaac acgacatccg caccataaat
    48961 agacttgcaa aaaatataac atcattgttt tcaatctgcc gtttatggga ttgaccgttt
    49021 tcttttgaca cggagttcaa caatgttcgg cataattata tctgtcatcg tattaattac
    49081 gatgggctat ttgatcctga aaaactacaa acctcaggtg gtgctggctg ccgcaggtgt
    49141 cttcctgatg atgtgcggag tctggttagg gttcggtggt gtactcgatc ccgccaaaag
    49201 cagcggctac ttgatcgtcg acatttataa tgaaatcctg cgcatgctgt ccaaccgcat
    49261 tgccggattg gggctgtcga ttatggcggt gggcggttat gcccgctaca tggagcgcat
    49321 aggagccagt cgcgcgatgg tgagcttgct aagccgcccg ttaaaactca ttcgctcgcc
    49381 gtatattatt ctgtcggcaa cttacgtcat cggccaaatc atggcgcagt ttattaccag
    49441 cgcctccggt ctgggtatgt tgctgatggt caccttattt ccgacgctgg tgagtctggg
    49501 agtaagtcgc ctctctgcgg tggcggttat cgcaaccacg atgtccattg agtgggggat
    49561 tctggaaacg aactccattt ttgcagccca ggtcgcggga atgaaaattg ccacatactt
    49621 cttccactac cagcttccgg tcgcctcttg cgtcattatc tcggtggcga tctcccactt
    49681 tttcgtgcaa cgcgcttttg acaaaaaaga taaaaatatc aatcacgaac aggcagagca
    49741 aaaaactctc gataatgtcc cgccgctcta ttacgccatt ttacctgtga tgccgttaat
    49801 cctgatgctc ggctcgctgt tcctcgccca cgtcgggctg atgaagtcag aactgcatct
    49861 ggtggtggtg atgttactaa gtttgacggt gacgatgttt gttgagttct tccgcaagca
    49921 taacttgcgc gaaacaatgg acgatgtgca ggcgtttttt gacggcatgg gtacgcagtt
    49981 tgccaacgtg gtaacgctgg tggtcgcggg tgaaatattt gcgaaaggct taacgacgat
    50041 tggcactgtt gatgcggtta tcaggggtgc ggagcattct ggtctgggcg gtattggcgt
    50101 gatgattatt atggcgctag tcattgccat ttgtgccatt gtgatgggct ctggcaatgc
    50161 gccgtttatg tcatttgcca gtcttattcc gaatatcgca gccggactac atgtaccagc
    50221 ggttgtaatg attatgccga tgcattttgc cacgacgcta gcgcgcgcgg tttcgccgat
    50281 tactgcggtg gtgaagcgga cagcgatccc catggcagtc ggtttcgtgg tgaatatgat
    50341 tgccacaatc acgctatttt attaagtcat taaaaagaca aaacaggccg cctgggcctg
    50401 ttttgtatta cttcacaaca cgtaatgccg gtcgaccacc gcgtggtggc tgcggaggtt
    50461 catcgtcagg atgagtgtca tcatcgtgat ctggcttgtc gccatcaata accgacataa
    50521 cggtttcgtt gtctgccgat gcctcttcat cattcatgat gctggtatct tcatcgtagg
    50581 cagcttcagg ctcaaacatc gtgcctgcgc cattttcacg ggcgtagata gccagcacgg
    50641 cagccagcgg cacagaaacc tgacgcggaa tgccaccaaa gcgcgcgtta aagcgcacct
    50701 catcattcgc cagttccaga ttgccgacag cacgcggcgc aatgttgagt acgatttgcc
    50761 cgtcacgcgc atattccata ggaacctgca cgccagggag cgtcacatcc accaccaggt
    50821 gcggcgtgag ctggttatcc agcaaccact catagaatgc acgcagcaga tagggacgac
    50881 gtggtgttag ctgtgacaaa tccatacaga ttaactccgg cccagacaca tttcacgttc
    50941 tgcttcagtt aaagaagcaa ggaaagagtc acgctcaaag acgcgggtca tatagccttt
    51001 cagctctttc gcacccgggc cgctgagctc gatacccagt tgcggcagac gccacagcag
    51061 cggagcaaga tagcaatcga ccaggctgaa ctcatcgctc aggaagtacg gcttctgacc
    51121 gaagaccggc gcaatcgcca gcagttcttc gcgcagttgc ttacgtgcgg catctgcttc
    51181 agaagctgaa ccgttgatga tggtgttcat cagcgtgtac cagtcttttt cgatgcgatg
    51241 catgtacaga cggctttcac cgcgagctac cgggtaaaca ggcatcagtg gcggatgcgg
    51301 gaaacgctca tccagatatt ccataatgat gcgagattcc cacagggtca gctcacgatc
    51361 caccagggtc ggaacgctct gattcgggtt gaggtcaatc agatcctgag gcggattgtc
    51421 cttttccacg tgttcgatct cgaaacttac acctttctca gccagcacaa tgcggacctg
    51481 atggctatag atgtcagtag gaccggaaaa cagcgtcatt accgaacgtt tgttggcagc
    51541 gacagccatg aaaacctcca ggtatagtca gaatttttac tgctaccagc caccaggtgg
    51601 ccagtcagaa gttgtgttac ccaataagga acgactctct ttgttcgaaa atcaaacaaa
    51661 aaatgagcaa tacccgacat ttgggcagaa aattggatga tagtttacca gattttgcga
    51721 ccattgtggt gagtggatgc cggaaatggg gaaaaagaga tgcgctttag tctgaaatag
    51781 ttgacttaga cccttattgg cgatgtggtt tttgttttgc ctgtctttca gacggcagca
    51841 aaaagcaact tcccattttt tacgctgatt cagaatttag ccataaaaaa acccgccgaa
    51901 gcgggttttt tcgtaaattg ttttctgccg gagcagaagc caattaacgt ttggagaact
    51961 gcggacgacg acgtgcttta cgcagaccga ctttcttacg ttcaacctga cgagcgtcac
    52021 gagtaacgaa gccagcttta cgcagttcag aacgcaggga ctcgtcgtat tccatcagag
    52081 cgcgggtgat accgtgacgg atcgcaccag cctgaccaga gataccacca cctttaacgg
    52141 tgatgtacag gtccagtttc tcaaccatgt cgaccagttc cagcggctga cgaactacca
    52201 tgcgggcagt ttcacgaccg aagtactgtt ccagagaacg ttggttgatt acgattttac
    52261 cgttgcccgg tttgatgaaa acgcgagctg cggaattttt gcggcgacca gtgccgtagt
    52321 attgattttc agccattgcc tataatcccg attagatgtc aagaacttgc ggttgctgtg
    52381 ccgcgtggtt gtgctcgtta cccgcgtaaa ctttcagttt acggaacata gcacgaccca
    52441 gcgggccttt tggcaacatg cctttaaccg cgatttcaat cacacgctca ggacggcgag
    52501 caatcatctc ttcaaagctc acttgtttga taccaccgat gtggccggtg tggtgatagt
    52561 acactttgtc agtacgcttg ttgccggtta cagcaacttt gtcagcgttc agaacgatga
    52621 tgtaatcacc ggtatctacg tgcggagtgt attccgcttt gtgcttaccg cgtaggcgac
    52681 gagccagttc agtagccaga cggcccagag ttttaccggt cgcgtcaaca acataccagt
    52741 cgcgttttac ggtttctggt ttagctgtaa aagttttcat taaaagctta cccaataaat
    52801 agttacacgt tggtgaacac ccaaacgtct tcaattgttg aggttcacac gacaaagtcc
    52861 ggcaaaccta ccccttcgaa tagcctatgc cagcacacaa aaagttttgg gaaaaaaact
    52921 ttcttgtaac gtggggtcgc aggattatag agaagtcggg gtcaaagatc gacccctttt
    52981 tgtgatttgt gacaggtttt aacccgccaa atgctcgcgc ttcagatact cttcgctttg
    53041 catctcttgc agacgtgaca ggcaacgctg gaactcaaac ttcagccgat cgccctgata
    53101 aatttcatac agcggcactt ctgcactcac cactaatttg acatggcgct cgtaaaactc
    53161 atccaccagc gcaataaagc gccgcgcttc gctctccatc aaccgcgtca taactggtac
    53221 atcaaacaac atgaccgtat gaaagagacg tgagagcgca atatagtcat gctgactgcg
    53281 ggcgtcgacg cacagcgtag taaaagagac cgccagcgtc tggttctcga cgcccattgt
    53341 cgctaatggc cgatggttga tttctaacgt cggtgaattt tctcgtttcc cccccgccag
    53401 cgccaaccat agtttatcca tttgcgcccg ggtttcatcg tggagtggcg aaagccacag
    53461 atgcgcctga gtgagtgtac gcagtcgata atcaacacca gcgtccacgt tcattacatc
    53521 acaatgctgt ttaatggcat cgattgcagg cagaaaacgc gcacgttgca agccatttcg
    53581 ataaagttcg tccggcggaa tatttgacgt cgctaccagg gtaataccgc gggcgaacaa
    53641 ggctttcatc agaccgccaa gtagcatggc atcggtaata tcagaaacaa aaaattcgtc
    53701 aaaacagagc acgtcagttt cggctttaaa gcgatcggca ataatttcca gcggatcggt
    53761 ctgcccctgt aaggcagtta gctcttcatg cacccgcagc ataaaacggt gaaagtgcag
    53821 gcgctgtttc cgctctcccg gcaggctttg atagaaaagg tccatcagcc aggttttccc
    53881 gcgtcctaca ccgccccaca tatataagcc acgcactggc gtatgctttg tgtcttcgcg
    53941 tttaccccac agcttaccga cccgcgccat tagcccactc gtcctggggg ctggtggcgt
    54001 gctattgatg agttcctgat aaataatttc caggcggctg acggcctctt tttgaacgtc
    54061 gtcgggttga tggctgcctt cattgagcgc cttcaggtat tgcgatgttg gggtaacgct
    54121 ttgcatgatc ttattgttat tccttgaata atcggtgcgc cgttgttcac ggttgacgaa
    54181 aaaaaggccg ttctacacta cgcgatatgc agtcgggatt ccacttctgt ggaattaacg
    54241 gttatagtgg cataatcagc cgcaggcatg gagcctgaag ccaacaccct acggaaacaa
    54301 aagacaacgg gagatgttca tgacctggga atatgcgcta attgggttag tcgtcggcat
    54361 cattattggt gctgtggcca tgcgttttgg taatcgtaaa ctacgccagc aacaggcgtt
    54421 gcagtacgaa ctggaaaaga ataaagctga actggacgag tatcgcgaag agctggttag
    54481 ccactttgcc cgcagcgcgg aattactgga taccatggcg cacgactatc gccagctgta
    54541 tcagcacatg gcgaaaagtt ccagcagcct gctgccggaa ctgtctgctg aagcaaaccc
    54601 gttccgtaat cgtctggcag agtctgaagc cagcaacgat caggcaccgg tgcagatgcc
    54661 tcgcgactat tctgaaggcg catccggcct gctgcgtact ggcgcgaagc gcgactaatt
    54721 tatttttcgg gcgcagccat tgcgccctcc tcttctctcc ctccccgact atcatttaat
    54781 ctggtgtctc attgttagcc gtctgaaaat tcaataacat caaactgttt tgaatctctt
    54841 ttcttatcat tcaggtacga gagcaggaat aatgaaaaaa caaccccagc tgttgagtgc
    54901 attagcgtta agtgtcgggt taactctctc ggcgtcattt caggccgtcg cgtcgattcc
    54961 aggccaggtt gccgatcagg cccctctccc cagtctggct ccaatgctgg aaaaagtgct
    55021 tccggcagtg gtgagcgtac gggtggaagg aacggccagt cagggacaga aaatcccgga
    55081 agaattcaaa aagttttttg gtgatgattt accggagcaa cctgcacaac ccttcgaagg
    55141 tttaggctcc ggtgtcatca tcaacgccag taaaggctat gtgctgacca acaaccatgt
    55201 gattaatcag gcacagaaaa tcagtattca gctcaatgat gggcgcgagt ttgatgcaaa
    55261 actgattggt agcgatgacc agagcgatat cgccctgtta caaattcaaa acccgagcaa
    55321 attaacgcaa atcgctattg ccgactccga taaattgcgc gtcggtgatt ttgccgtagc
    55381 ggtcggtaac ccatttggcc ttgggcaaac cgccacctct ggcattgttt ccgcattagg
    55441 acgcagcggg ttgaatcttg aaggtctgga aaactttatc cagacagatg cttccattaa
    55501 ccgcggtaac tccggcggtg cactgttaaa ccttaacggt gagttaattg gcatcaacac
    55561 tgcaatcctt gcgcctggcg gcgggagcgt cgggattgga tttgccatcc ccagtaatat
    55621 ggcgcgaaca ctggcgcagc agcttatcga ctttggtgaa atcaaacgcg gtttgttagg
    55681 catcaaaggc accgagatga gtgccgatat cgccaaagcc ttcaaccttg acgtgcagcg
    55741 tggcgcgttt gtcagcgaag tgttgccagg ttctggctcg gcgaaagcgg gcgtcaaagc
    55801 gggcgatatt attaccagcc tcaacggcaa accgctgaat agctttgctg agttgcgctc
    55861 tcgtatcgcg accaccgagc cgggcacaaa agtgaaactt ggcctgctgc gtaacggcaa
    55921 accactggaa gtagaagtga cgctcgatac cagcacctct tcgtcggcca gcgctgaaat
    55981 gatcgcgcca gcgctggaag gtgcaacgtt gagcgatggt cagctaaaag atggcggcaa
    56041 aggtattaag atcgatgaag ttgtcaaagg aagcccagct gctcaggctg gcttgcaaaa
    56101 agacgatgtg atcattggcg tcaaccgcga tcgggtgaac tcgattgctg aaatgcgtaa
    56161 agtgctggcg gcaaaatcgg ccatcatcgc cctgcaaatt gtacgcggca atgaaagcat
    56221 ctatctgctg atgcgttaat gtcgtaaacc gggcatcagg cttacgtgtg atgtccggtt
    56281 aactcgtggt atgctgctgc cgttcccttt tttaatgacg cctccatcat gtttgtgaag
    56341 ctcttacgtt ccgttgcgat tggattaatt gtcggcgcta ttctgctggt tgccatgcct
    56401 tcgctgcgca gccttaaccc gctttccact ccgcaatttg acagtaccga tgagacgcct
    56461 gccagctata atctggcggt tcgccgcgcc gcgccagcgg tggttaacgt ttacaaccgt
    56521 ggtttgaaca ccaactctca caaccagctt gagatccgca ccctgggatc cggtgtaatc
    56581 atggatcaac gcggttatat catcaccaat aaacacgtca tcaacgacgc cgatcagatc
    56641 atcgtcgcct tacaggatgg tcgtgtattt gaagcattgc tggtgggatc tgactctcta
    56701 accgatctgg cggtacttaa aattaatgcc actggcggtt tacctaccat tccaatcaat
    56761 gcacgtcgcg taccgcatat tggcgacgtg gtactggcga tcggtaaccc gtacaacctc
    56821 gggcagacca ttacccaggg gattattagt gccacgggtc gaattggtct gaacccgacc
    56881 gggcggcaaa acttcctgca aaccgatgct tccattaacc acggtaactc tggcggcgcg
    56941 ctggtgaact cgctgggcga actgatgggc attaacacgc tgtcgtttga taagagtaac
    57001 gatggcgaaa cgccggaagg tatcggcttt gcgattcctt tccagttagc aaccaaaatt
    57061 atggataagc tgatccgcga tggtcgcgtg atccgcggct acattggcat tggcggacgt
    57121 gagatcgcac cgctgcacgc acagggcggt ggtatagatc aactgcaagg gatcgtggtt
    57181 aatgaagtgt cacctgacgg cccggcggcg aatgcgggta ttcaggtcaa cgatctgatt
    57241 atttcggtgg ataacaaacc ggccatctct gctctggaga cgatggatca ggtggcggaa
    57301 attcgccctg gttcggtgat cccggttgta gtgatgcgtg atgataagca gttaacgctg
    57361 caggtcacca ttcaggaata tccggcaacc aattaagtcg agtgctcaaa acaaaaaacc
//
LOCUS       DAJVQG010000009        46590 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJVQG010000009 DAJVQG010000000
VERSION     DAJVQG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46590
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.009"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(186..779)
                     /locus_tag="OQM83_000513"
     CDS             complement(186..779)
                     /locus_tag="OQM83_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCS1518865.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            850..1563
                     /gene="bdcA"
                     /locus_tag="OQM83_000514"
     CDS             850..1563
                     /gene="bdcA"
                     /locus_tag="OQM83_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1518866.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1694..2089
                     /locus_tag="OQM83_000515"
     CDS             1694..2089
                     /locus_tag="OQM83_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCS1518867.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2370..2504
                     /gene="pyrL"
                     /locus_tag="OQM83_000516"
     CDS             2370..2504
                     /gene="pyrL"
                     /locus_tag="OQM83_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCS1518868.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2508..3443
                     /gene="pyrB"
                     /locus_tag="OQM83_000517"
     CDS             2508..3443
                     /gene="pyrB"
                     /locus_tag="OQM83_000517"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCS1518869.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3456..3917
                     /gene="pyrI"
                     /locus_tag="OQM83_000518"
     CDS             3456..3917
                     /gene="pyrI"
                     /locus_tag="OQM83_000518"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCS1518870.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            3990..4376
                     /gene="ridA"
                     /locus_tag="OQM83_000519"
     CDS             3990..4376
                     /gene="ridA"
                     /locus_tag="OQM83_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCS1518871.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4582..7278)
                     /gene="mgtA"
                     /locus_tag="OQM83_000520"
     CDS             complement(4582..7278)
                     /gene="mgtA"
                     /locus_tag="OQM83_000520"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCS1518872.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7419..7472)
                     /gene="mgtL"
                     /locus_tag="OQM83_000521"
     CDS             complement(7419..7472)
                     /gene="mgtL"
                     /locus_tag="OQM83_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCS1518873.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7657..8604
                     /gene="treR"
                     /locus_tag="OQM83_000522"
     CDS             7657..8604
                     /gene="treR"
                     /locus_tag="OQM83_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCS1518874.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8723..10144
                     /gene="treB"
                     /locus_tag="OQM83_000523"
     CDS             8723..10144
                     /gene="treB"
                     /locus_tag="OQM83_000523"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCS1518875.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10194..11849
                     /gene="treC"
                     /locus_tag="OQM83_000524"
     CDS             10194..11849
                     /gene="treC"
                     /locus_tag="OQM83_000524"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCS1518876.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12243..14381
                     /gene="nrdD"
                     /locus_tag="OQM83_000525"
     CDS             12243..14381
                     /gene="nrdD"
                     /locus_tag="OQM83_000525"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCS1518877.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14539..15003
                     /gene="nrdG"
                     /locus_tag="OQM83_000526"
     CDS             14539..15003
                     /gene="nrdG"
                     /locus_tag="OQM83_000526"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCS1518878.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15048..15434)
                     /gene="cybC"
                     /locus_tag="OQM83_000527"
     CDS             complement(15048..15434)
                     /gene="cybC"
                     /locus_tag="OQM83_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCS1518879.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15618..16970)
                     /gene="pmbA"
                     /locus_tag="OQM83_000528"
     CDS             complement(15618..16970)
                     /gene="pmbA"
                     /locus_tag="OQM83_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCS1518880.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17064..17615
                     /locus_tag="OQM83_000529"
     CDS             17064..17615
                     /locus_tag="OQM83_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCS1518881.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17698..19071)
                     /gene="mpl"
                     /locus_tag="OQM83_000530"
     CDS             complement(17698..19071)
                     /gene="mpl"
                     /locus_tag="OQM83_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCS1518882.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19247..20245
                     /gene="fbp"
                     /locus_tag="OQM83_000531"
     CDS             19247..20245
                     /gene="fbp"
                     /locus_tag="OQM83_000531"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCS1518883.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20278..21273)
                     /gene="yjfF"
                     /locus_tag="OQM83_000532"
     CDS             complement(20278..21273)
                     /gene="yjfF"
                     /locus_tag="OQM83_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCS1518884.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21260..22282)
                     /locus_tag="OQM83_000533"
     CDS             complement(21260..22282)
                     /locus_tag="OQM83_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1518885.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22296..23798)
                     /locus_tag="OQM83_000534"
     CDS             complement(22296..23798)
                     /locus_tag="OQM83_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCS1518886.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(23938..24894)
                     /gene="ytfQ"
                     /locus_tag="OQM83_000535"
     CDS             complement(23938..24894)
                     /gene="ytfQ"
                     /locus_tag="OQM83_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HCS1518887.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25204..25734
                     /gene="ppa"
                     /locus_tag="OQM83_000536"
     CDS             25204..25734
                     /gene="ppa"
                     /locus_tag="OQM83_000536"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCS1518888.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26113..26454)
                     /locus_tag="OQM83_000537"
     CDS             complement(26113..26454)
                     /locus_tag="OQM83_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCS1518889.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26457..30236)
                     /gene="tamB"
                     /locus_tag="OQM83_000538"
     CDS             complement(26457..30236)
                     /gene="tamB"
                     /locus_tag="OQM83_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCS1518890.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30233..31966)
                     /gene="tamA"
                     /locus_tag="OQM83_000539"
     CDS             complement(30233..31966)
                     /gene="tamA"
                     /locus_tag="OQM83_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCS1518891.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32172..32810
                     /gene="msrA"
                     /locus_tag="OQM83_000540"
     CDS             32172..32810
                     /gene="msrA"
                     /locus_tag="OQM83_000540"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCS1518892.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33133..34476
                     /locus_tag="OQM83_000541"
     CDS             33133..34476
                     /locus_tag="OQM83_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCS1518893.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34556..34762)
                     /locus_tag="OQM83_000542"
     CDS             complement(34556..34762)
                     /locus_tag="OQM83_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCS1518894.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35087..35641
                     /locus_tag="OQM83_000543"
     CDS             35087..35641
                     /locus_tag="OQM83_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCS1518895.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35731..36642)
                     /locus_tag="OQM83_000544"
     CDS             complement(35731..36642)
                     /locus_tag="OQM83_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1518896.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICKKYKSTIEAFKKEQLALPV"
     gene            complement(36854..37594)
                     /gene="cysQ"
                     /locus_tag="OQM83_000545"
     CDS             complement(36854..37594)
                     /gene="cysQ"
                     /locus_tag="OQM83_000545"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCS1518897.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            37784..39727
                     /gene="cpdB"
                     /locus_tag="OQM83_000546"
     CDS             37784..39727
                     /gene="cpdB"
                     /locus_tag="OQM83_000546"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCS1518898.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(39845..40225)
                     /locus_tag="OQM83_000547"
     CDS             complement(39845..40225)
                     /locus_tag="OQM83_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCS1518899.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            40314..41174
                     /gene="qorB"
                     /locus_tag="OQM83_000548"
     CDS             40314..41174
                     /gene="qorB"
                     /locus_tag="OQM83_000548"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCS1518900.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            41282..42247
                     /locus_tag="OQM83_000549"
     CDS             41282..42247
                     /locus_tag="OQM83_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1518901.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            42355..43017
                     /gene="ytfE"
                     /locus_tag="OQM83_000550"
     CDS             42355..43017
                     /gene="ytfE"
                     /locus_tag="OQM83_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCS1518902.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(43062..44474)
                     /gene="cycA"
                     /locus_tag="OQM83_000551"
                     /gene_synonym="dagA"
     CDS             complement(43062..44474)
                     /gene="cycA"
                     /locus_tag="OQM83_000551"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCS1518903.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(44783..45403)
                     /gene="fklB"
                     /locus_tag="OQM83_000552"
     CDS             complement(44783..45403)
                     /gene="fklB"
                     /locus_tag="OQM83_000552"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1518904.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            45622..46260
                     /gene="ytfB"
                     /locus_tag="OQM83_000553"
     CDS             45622..46260
                     /gene="ytfB"
                     /locus_tag="OQM83_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HCS1518905.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(46244..>46590)
                     /locus_tag="OQM83_000554"
     CDS             complement(46244..>46590)
                     /locus_tag="OQM83_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCS1518906.1"
                     /translation="PWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGKK
                     HNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILS
                     WLLVDPSSTAHE"
BASE COUNT        11437 a        12390 c        11609 g        11154 t
ORIGIN      
        1 ggtagccacg gctgaagatt atgaatattt ccgatgatca tcttttccac tccttaatta
       61 aaacaatgtt tcatttccat cgttatacga aaagatgatc aattccgcta gccttaatgc
      121 gtgaattatt gtcttatcac aacttatcgg caggcgcgtt cccgcctttc gggtcaaggc
      181 ccgcatcact ccttgagaat agtttttatc gcttcccccg ccagtgcagc tgttgcacag
      241 agttgttcga tggagtgccc ctcccgagcc ttcgcagaaa gccctgacat cacggtactc
      301 ataaaatcag tcacacattg tgcgctttgt ggatgccgcc tggcgatata gtcataaatg
      361 gccgtttctg cggcgtgata atattgaacg gcaatatcac gcgcttgtgg atcatgacta
      421 tgaatgcctt caagaaccat acagccagcg cagccgccgt tttggctata tctgcgcgcc
      481 gcttctttta atacctcagc caggcactcc cctaccggac gatcatcacg aagaatatcg
      541 gcaagcggaa tagcttccgt accgacatat tcattgagta cacggctaaa taacccagcc
      601 ttactgccaa aagccgcgta gaggctcggg gggttaatac caagataatc agtaacttca
      661 gcaacactga cagcatcgaa acctttttgg tgaaaaagca cttttgccgc agagactgcc
      721 tgctcaggag cgaaacgtct gggacgacct ggaacgcgag attgtttttt agtgaccatt
      781 acaaaacttg ttgacagaaa gttaaaacag ttttgtaatg catgttacat aataaatcaa
      841 ggagtcctta tgggcgcttt tacaggtaag acagttctca tcctcggtgg cagtcgtggt
      901 atcggtgccg ctatcgtacg tcgtttcgtc accgatgggg ccaatgtacg attcacctat
      961 gcggggtcga aagatgccgc tgaacacctg gcacaaaaga ctggagcgac agcagtattc
     1021 acagatagtg ctgacagaga cgctgtcatt gatgtcgttc gtaagagcgg cgcattggat
     1081 atcctggtgg taaatgcagg tattggcgtc tttggcgatg ccctggaatt gaatgccgac
     1141 gatattgatc gccttttcaa aatcaatatt catgctcctt atcatgcctc tgttgaagcc
     1201 gcccggcaga tgcccgaagg cgggcgcatc ttaatcatcg gctccgtgaa tggcgatcgt
     1261 atgcctgttg caggcatggc tgcttatgcc gccagtaaat ctgccctgca aggcatggcg
     1321 cgcgagctgg cccgtgattt cggaccgcgt gggatcacca tcaacgtcgt ccagccaggg
     1381 ccaattgata ccgacgctaa tcccgccaac gggccaatgc gcgatatgtt gcatagtttt
     1441 atggctatca aaagacatgg gcaaccggaa gaggtcgctg gtatggtcgc atggttagca
     1501 gggccagaag ccagttttgt taccggcgcg atgcatacca ttgatggcgc gtttggcgca
     1561 taaccgacta cgctcaatta agcccagcca tttcccatga tgtctgggtt ttgtttactc
     1621 acgtcgtccg ctaaaagcgg ctcctggtaa atataaatct tctgttcatg tcgtaaatct
     1681 tgaggataac caaatggtag aaagaaccgc tgttttccct gcaggccgac attcactgta
     1741 tgctgaacat cgttattctg cggctattcg ttccggcgat ttgctgtttg tttccgggca
     1801 agtaggaagt cgagaggacg gaacaccaga acccgatttt cagcaacaag tcagactggc
     1861 atttgataat ttgcatgcga ccctggctgc tgcgggatgc acttttgacg atatcattga
     1921 tgttacgagc ttccataccg atccagaaaa ccaatttgaa gacatcatga cggtgaaaaa
     1981 tgaaatattt agcgccccac cttatccaaa ctggacggcg gtaggtgtta catggctggc
     2041 aggctttgat tttgaaatta aagtgatagc gcgcatccct gagcagtaag caatagtgtt
     2101 agccgttcgt tttcacactc cgccctataa gtcggatgaa tggaataaaa tgcatatctg
     2161 attgcgtgaa agtgaaaaag gaaaaagcag gaaatgtctg caattattga taccgaacga
     2221 ccgttcccct gcagaatcac aacaaataaa aatgcatgta ttttgactta taattcaaat
     2281 aaaccgtttg cgctgacaaa atattgcatc aaatgcttgc accgcttctg acgatgagta
     2341 taatgccgga caatttgccg ggaggatgta tggttcagtg tgttcgacat tttgtcttac
     2401 cgcgtctgaa aaaagacgct ggcctgccgt ttttcttccc gttgatcacc cattcccagc
     2461 ccctcaatcg aggggctttt ttttgcccag gcgtcaggag ataaaagatg gctaatccgc
     2521 tatatcagaa acatatcatt tccataaacg accttagtcg cgatgacctt aatctggtgc
     2581 tggcgacagc ggcgaaactg aaagcaaacc cgcaaccaga gctgttgaag cacaaagtca
     2641 ttgccagctg tttcttcgaa gcctctaccc gtacccgcct ctctttcgaa acttccatgc
     2701 accgcctggg tgccagcgtg gtgggcttct ccgacagcgc caatacatca ctgggtaaaa
     2761 agggcgaaac gctggccgat accatttcgg ttatcagcac ttacgtcgat gcgatagtga
     2821 tgcgtcatcc gcaggaaggt gcggcgcgcc tggccaccga gttttccggc aatgtaccgg
     2881 tactgaatgc cggtgatggc tccaaccaac atccgacgca aaccttgctg gacttattca
     2941 ctattcagga aacccagggg cgtctggaca atctccacgt cgcaatggtt ggtgacctga
     3001 aatatggccg caccgttcac tccctgactc aggcgttagc gaagttcgac ggcaaccgtt
     3061 tttacttcat cgcgccggac gcgctggcaa tgccgcaata cattctggat atgcttgatg
     3121 aaaaagggat cgcctggagt ctgcacagct ctattgaaga agtgatggcg gaagtagaca
     3181 tcctgtacat gacccgcgtg caaaaagagc gtctggaccc gtccgagtac gccaacgtga
     3241 aagcgcagtt tgttcttcgc gccagcgatc tccacaacgc caaagccaat atgaaagtgc
     3301 tgcatccgct gccgcgtgtt gatgagattg cgacggatgt tgataaaacg ccacacgcct
     3361 ggtacttcca gcaggcaggc aacgggattt tcgctcgcca ggcgttactg gcactggttc
     3421 tgaatcgcga tctggtactg taaggggaaa tagagatgac acacgataat aaattgcagg
     3481 ttgaagctat taaacgcggc acggtaattg accatatccc cgcccagatc ggttttaagc
     3541 tgttgagtct gttcaagctg accgaaacgg atcagcgcat caccattggt ctgaacctgc
     3601 cttctggcga gatgggccgc aaagatctga tcaaaatcga aaataccttt ttgagtgaag
     3661 atcaggtaga tcaactggca ttgtatgcgc cgcaagccac ggttaaccgt atcgacaact
     3721 atgaagtggt gggtaaatca cgccccagtc tgccggagcg catcgacaat gtgctggtct
     3781 gcccgaacag caactgtatc agccatgccg aaccggtttc ttccagcttt gccgtgcgaa
     3841 aacgcgccaa tgacatcgcg ctcaaatgca aatactgtga aaaagagttt tcccataatg
     3901 tggtgctggc caattaattg cggttggtaa taaaagtctg gctccctata atgagccaga
     3961 ctttttaccg ctgtaataaa ggagaaatca tgagcaaaac tatcgcgacg gaaaatgcac
     4021 cggcagctat cggtccttac gtacagggcg ttgatctggg caatatgatc atcacctccg
     4081 gtcagatccc ggtaaatccg aaaacgggcg aagtaccggc agacgtcgct gcacaggcac
     4141 gtcagtcgct ggataacgta aaagcgatcg tcgaagccgc tggcctgaaa gtgggcgaca
     4201 tcgttaaaac caccgtgttt gtgaaagatc tgaacgactt cgcaaccgtt aatgctacct
     4261 acgaagcctt cttcaccgaa cacaatgcca ccttcccggc acgttcttgc gttgaagttg
     4321 cccgtctgcc gaaagacgtg aaaattgaga tcgaagcgat cgctgttcgt cgctaatctt
     4381 gatggaaatc cgggctatca tgcccggatt aagtctgatg acaaacacaa aatcgcctga
     4441 tgcgctacgc ttatcaggcc tacgtgattc ctgcaattta ttgaatttgt tggccggata
     4501 aggcatttac gccgcatccg gcatgaacaa aactcacttt gtctgcaatc tgaatccagg
     4561 ccaccatgcc cagttgattc tttattgcca gccgtaacga cggctataaa atcctttcac
     4621 cagttgggtt aacgtcatat accctgccag aatcgcaacc agccacggga aatagcttaa
     4681 cggcagcgcc tgcagttgca gataactggc cagcggtgaa aacggcaatg cgatcccgac
     4741 aatcatcacg atcacggtca tgatcattaa cggccacgat gcacagctct gaataaacgg
     4801 cacacggcgg gtgcggatca tatgcacaat cagcgtttgc gacagcaagc ccaccacaaa
     4861 ccatcccgac tggaacagcg tttgcgtttc cggcgtgttg gcatggaata cccaccacat
     4921 caggcaaaac gtcagaatat cgaagatcga gctgatcggt ccgaagaaga tcataaagcg
     4981 ccccagatcc gccggattcc aacgctgcgg cttttgaatt tgctcgtcgt cgacgttatc
     5041 aaacgggatc gccacctgtg acacatcata cagcaggttc tgaataagca agtgtaacgg
     5101 caacatcggc aggaagggca agaaagcact cgccaccagc acgctgaaca cattaccgaa
     5161 gttggagctc gccgtcattt tgatgtattt cagcatgttg gcgaaagtgc gacgtccttc
     5221 aataaccccc tcttccagca ccatcaggct tttttccagc aggatgatat cagccgcttc
     5281 acgggcaata tctaccgcgc cgtccacaga aatgccgata tccgcagcgc gtaaggcggg
     5341 cgcatcatta ataccatcgc ccataaagcc aaccacatgc ccttcgcgct tcagtaaggt
     5401 cacaatgcgt tctttatgca tcggcgtcag acgagcaaac agcgtggtgc gctgtgcgag
     5461 atttgccagt tcgtcgtcag atagcgtttc aatatcacta ccaatgacca cctctcccgc
     5521 gtccagcccc acttcatggc acactttcgc tgcaactaac tcactgtcgc cagtaaggat
     5581 ttttacggta attccactcg cttttaatgc cttcagtgcc ggagcagttg tctcttttgg
     5641 cggatcaagg aaagcaatat atccttcgag gatcaggtcg gattcatccg cccgctggta
     5701 atctccttca cgcgctggca ggtatttcgt cgccaccgca accacgcgca gcccctgacg
     5761 attcagcgta tcagtaaccc gcttaatctt acgcagcatg atgtcatcga gcggcacaat
     5821 ctcgccattg tgacgcacct gcgaacacac attgaggatt tcctgcaatg cacctttgca
     5881 aaccagctga tggtgctcgg tattttctgc cactaccacc gacatccggc gacgctcgaa
     5941 atcaaacgga atctcatcaa ttttttgcca acggctggcc agagagcgcg ctgactcttc
     6001 atccgtacct tcgagcaccg ctgtatcgag taggttttta agtccggtct gataatgact
     6061 gttcaaccac gcgctatgca gcacgcgttc gctggtttta ccggagatat cggtatgatt
     6121 ctccagcaca attttgtcct gtgtcagggt gccagtttta tcagtgcaca gaatatccat
     6181 tgcgccaaag ttctgaatag catccagatg tttgacgatc actttctgtt tcgacagctt
     6241 tactgcgccg cgcgccagcg tcgaggtaac aatcatcggc aacatttccg gcgttaagcc
     6301 taccgcgacc gaaagcgcaa acagtgccgc ttcccaccag tcgcctttgg tgtaaccgtt
     6361 gataagcaac accaccggag ccatcaccag cataaagcga atcagcagca tactgacgcg
     6421 gctgatcccc tgctgaaagg cattcggctc gctttcctgc tcactaacac gccccgccag
     6481 ttgaccaaac caggtgttgg ctcctgtagc aatcaccatt gcttgtgccg tgccgctcac
     6541 cacggtggtg cccataaaac acagggtgtc acactccagc ggattgctgt gctccggctg
     6601 gcgagtggta gcggcttttt ccacgggcag agactcaccg gttaacgacg cctgagcgac
     6661 gaacagatcc cgcgcctgca agatacgtaa atctgccggg atcatatccc ccgccgccag
     6721 ttttataata tcgccgggca ccagctggtc gatcgggatc tccagccagc cattttcgcc
     6781 tttgtcgtta attacgcgca gcaccgtcgc ggtattgctg accatcgctt tcagggcatc
     6841 tgccgcttta gtggaacgtg cttcctgaat aaagttcagc aacgtagaaa tagcgaccat
     6901 tagcgcgata acgcccgcgg caaataaatc ttcagtggcg taagaaatag cgccgagaat
     6961 ggtgagtaaa atattaaatg ggttgcgata gcagacccat aaatgtaccc accacggcga
     7021 cggttgttgt gcgggtaatt tattttcacc atgttgttcg cgggcagatt ccacttccgc
     7081 ctgatttaac ccttccggat gggtgtcgaa cgttttccac aattcttctt cgggcatcac
     7141 cgccattttc aggcaatgcg cacttaagga cggcgggacc gccgtattca ctgtctgctg
     7201 cgcgccaggc aatggatcgc gatgaaccag acgggaaggt aaatggcgaa tgagccgggt
     7261 aaaaatttct ttaaacataa ggagtccctc cgcactgtct gaaacagtac agaaatgtct
     7321 tcaggcgtgg cgaagcctta cctgaaggta ataaagctga gcagggacgt taaaacgtca
     7381 cggccttacg catccggtag cgccgtgaaa agacgggctt accggaaaag ttttaatctc
     7441 cgtcgaggga gaggcgtggg atcaggttcc atataacctc cggtaagtga aaaaagcgcc
     7501 gcaaataata aggagaatcc aataatattc gtagcattta tggcgatata atacttgctg
     7561 gcaactaaac caacgataaa ccagacttta cccattgctg aatgcacggg taaagttagg
     7621 ctcaaataat taaacaacac gttacaggac aacaggatgc aaaatcggct gactatcaaa
     7681 gacatcgcac gcttaagcgg cgtggggaaa tctacagttt cccgggtgct gaataacgaa
     7741 agcggcgtga gccagcgcac ccgcgagcgt gttgaagcag tgatgaatca gcatggattt
     7801 tccccttccc gctctgcgcg cgctatgcgt gggcaaagcg ataaagtggt cgccatcatt
     7861 gttacccgtc tggattcgtt gtcagaaaat ctcgccgttc aaaccatgct gccagcgttc
     7921 tatgaacaag gttacgaccc aatcatgatg gaaagccagt tttccccgca attagttgcc
     7981 gaacatttgg gggtgctgaa acggcgtaat atcgacggcg tagtgctgtt cggttttact
     8041 ggcataacag aagaaatgtt agcccactgg cagtcatcgc tggttctgct ggcgcgtgac
     8101 gcaaaaggct ttgcttcggt ctgttatgac gacgaagggg caatcaagat cctgatgcaa
     8161 cggctgtatg accaggggca tcgtaatatc agttatctcg gcgtgccgca tagtgacgtg
     8221 acaaccggta agcgacgtca cgaagcctac ctggcgttct gcaaagcgca taaactgcat
     8281 cccgttgccg ctctgccagg gcttgctatg aagcaaggct atgagaacgt ggcaaaagtg
     8341 attacgcctg aaactactgc gttactgtgc gcaaccgaca cgctggcact tggcgcaagt
     8401 aaatacctgc aagagcaacg catcgacacc ttgcaactgg cgagcgtcgg taatacaccg
     8461 ttaatgaaat tcctccatcc ggagatcgtc accgtagatc ccggttacgc cgaagctgga
     8521 cgccaggcgg cctgccaatt gatcgcacag gtaaccgggc gcagcgaacc gcaacaaatc
     8581 atcatccccg ccaccctgtc ctgatcgttt cctgaacgat aaattgtgat cttcgctgca
     8641 tttcgggaac gttcccgttt ttaaattttt ccgcgcaata tattctgcag ccaaccaaaa
     8701 atgtcatctg ccatggggct ttatgatgag caaaataaac caaacggata tcgatcggtt
     8761 gattgaactg gtcggcgggc gcggcaatat tgcgacggtg agccactgta ttactcgcct
     8821 gcgctttgtc ctcaaccaac cggccaatgc cagaccgaaa gaaattgagc aactccccat
     8881 ggtgaaaggc tgtttcacca atgccgggca atttcaggtg gtgattggca ccaacgtggg
     8941 tgattactat caagcactaa tagcgtcaac cggacaggcg caggttgata aagagcaggt
     9001 aaaaaaagcc gcccggcaga atatgaaatg gcatgagcag ttgatctctc atttcgcgga
     9061 gatcttcttc ccgttgctgc ccgcgttgat tagcggcggt ttgatcctcg gttttcgcaa
     9121 tgtgatcggc gatttgccca tgagcaacgg tcagacgctg gcgcaaatgt acccttccct
     9181 gcaaacgatc tacgattttc tgtggttgat cggtgaagcg attttcttct acctgccggt
     9241 cgggatttgc tggtcagcgg tgaaaaaaat gggcggcacg ccgatccttg gtatcgtgct
     9301 tggcgtgaca ctggtttccc cccagctgat gaacgcttat ctgctcgggc agcagctgcc
     9361 ggaagtgtgg gactttggca tgttcagcat cgccaaagtg ggctatcagg cgcaggtgat
     9421 cccggcactg ttagccgggc tggcgctggg cgttattgaa actcgcctta aacgcatcgt
     9481 accggattac ctctatctgg tggtggtgcc cgtctgttcg ctgatcctcg cggtgttcct
     9541 cgcccatgcg ctgattggtc cgtttggtcg catgattggc gatggcgttg cctttgcggt
     9601 acgtcacctg atgaccggca gctttgctcc gattggtgcg gcattgtttg gcttcctgta
     9661 cgcgccgctg gtgatcaccg gcgtacacca gaccaccctt gctattgatt tgcagatgat
     9721 tcaaagcatg ggtggcacgc cagtgtggcc gctgattgcg ctgtcgaata tcgctcaggg
     9781 ctccgccgtg ataggcatta tcatttccag ccgcaagcac aatgaacgcg agatctccgt
     9841 gcctgccgct atctccgcct ggcttggggt cactgagcct gcaatgtacg gcatcaacct
     9901 gaaatatcgc ttcccgatgc tgtgcgcgat gattggttct ggtctggcag gattgctatg
     9961 cggcctgaac ggcgttatgg cgaatggtat cggcgtaggg ggcctgccgg gaattctctc
    10021 gattcaaccg agctactggc aggtatttgc gctggcaatg gctatcgcca tcatcatccc
    10081 gattgtactc acctcgttta tctatcagcg gaaataccgc ttgggcacgc tggacattgt
    10141 ttaattttct tcggggcgca attgcgcccc ctcgcatttg caggaataac gtaatgacta
    10201 atcttcccca ctggtggcaa aacggcgtta tctaccagat ttatccaaag agttttcagg
    10261 acaccacggg tagcggcacc ggcgatttac gtggcgttat ccaacgcctg gactatctgc
    10321 ataaactggg cgttgatgcc atctggctaa ccccctttta tgtctctccc caggtcgata
    10381 acggttacga tgtagcgaac tatacggcga ttgatcccac ctacggcacg ctggacgatt
    10441 ttgacgaact ggtgacgcag gcaaaatcgc gcgggattcg tatcattctc gatatggtgt
    10501 ttaaccatac ctctacccaa catgcctggt ttcgcgaggc gctgaacaaa gaaagccctt
    10561 accgccagtt ttatatctgg cgcgatggcg aaccagaaac gccaccgaac aactggcgtt
    10621 caaaatttgg cggtagtgcg tggcgctggc atgcggaaag cgaacagtac tatttgcatc
    10681 tctttgcacc agaacaggcg gatctcaact gggagaatcc agcggtacgc gcagagctga
    10741 aaaaagtctg tgaattctgg gccgatcgtg gggtcgacgg gttgcgcctg gatgtggtga
    10801 atctgatctc caaagacccg cgtttccctg aagacctgga tggcgacggg cgtcgcttct
    10861 acaccgacgg gccacgagca cacgagtttt tgcacgagat gaaccgcgat gtgtttacgc
    10921 cacgcgggtt aatgaccgta ggtgaaatgt cctccaccag ccttgagcat tgccagcgat
    10981 acgcggcact gacaggcagt gaattgtcga tgacctttaa ttttcatcac ctgaaggtcg
    11041 attatcccgg tggtgaaaaa tggacgctgg ctaaacctga ctttgtggcg ttgaaaacat
    11101 tgttccgcca ctggcaacaa ggaatgcaca acgtggcatg gaatgccttg ttctggtgta
    11161 accacgatca gccgcgcatt gtttctcgct ttggtgatga aggtgaatac cgcgtgcctg
    11221 cggcaaaaat gctggcgatg gtgctgcatg gcatgcaggg aacgccgtat atctaccagg
    11281 gcgaagagat tggcatgacc aacccgcatt tcacgcgcat tactgactat cgcgacgtgg
    11341 agagcctcaa tatgtttgcc gagctgcgca acgatggtcg tgatgccgac gagttattgg
    11401 caatcctcgc cagtaaatcc cgtgacaaca gccgcacgcc catgcaatgg agcaacggcg
    11461 ataatgccgg gtttacggct ggcgaaccgt ggattggcct gggcgataac tatcaacaaa
    11521 tcaacgtaga agccgcgctg gccgatgact cctcggtgtt ttacacctac caaaagttaa
    11581 tcgcactgcg taagcaagaa gccatcctga catggggcaa ttaccaggat ctgctgccaa
    11641 acagccctgt attgtggtgc tatcgccgtg aatggaaggg gcaaaccttg ctggtcattg
    11701 ccaaccttag ccgtgagatc caaccctggc agccagggca aatgcgcggc aactggcagc
    11761 ttgtgatgca taactacgaa gaagcctcac cacaaccctg tgccatgaat ttacggcctt
    11821 ttgaggctgt ctggtggtta cagaagtaaa tcttccctaa gccccggtaa tgccggtcat
    11881 tccggggttt tgtctgtcag catgttgttt ttgttgattt ttcaaccagc aaattcatta
    11941 aaaaatttac ataacgctgt agcgcccgtc atccgtacgc tctgcttttt actttgagct
    12001 acatcaaaaa aagttaaaac atccttgatg caaagcacta tatatagact ttaaaatgcg
    12061 tcccaaccca atatgttgta ttaatcgact ataattgcta ctacagctcc ccacgaaaaa
    12121 ggtgcggcgt tgtggataag cggatggcga ttgcggaaag caccggaaaa cgaaacgaaa
    12181 aaaccggaaa acgcctttcc caatttctgt ggataacctg ttcttaaaaa tatggagcga
    12241 tcatgacacc gcatgtgatg aaacgagacg gctgcaaagt gccgtttaaa tcagagcgca
    12301 tcaaagaagc gattctgcgt gcagctaaag cagcggaagt cgatgatgcc gattattgcg
    12361 ccactgttgc cgcggttgtc agcgagcaga tgcagggccg caaccaggtg gatatcaacg
    12421 agatccagac cgcagttgaa aaccagctga tgtcgggtcc atacaaacaa ctggctcgtg
    12481 cttacatcga gtaccgtcac gatcgcgaca ttgaacgtga aaaacgcggt cgcctgaacc
    12541 aggagatccg tggtctggtc gagcagacca acgcctcgtt actcaacgaa aacgccaaca
    12601 aagacagcaa ggtgattcca acccagcgcg acctgctggc cgggatcgtg gctaaacact
    12661 atgcacgcca gcacctgctg ccgcgtgacg tggtgcaggc acatgagcgt ggcgatattc
    12721 actatcacga tctcgattac tcgccgttct tcccgatgtt caactgcatg ttgatcgacc
    12781 tgaaaggcat gctgacccag ggctttaaaa tggggaacgc cgagattgaa ccgccgaagt
    12841 cgatctctac ggcaaccgcg gtaactgcgc agattattgc tcaggtcgcc agccatattt
    12901 atggcggcac caccattaac cgtatcgatg aagtgctggc gccgtttgtt accgccagct
    12961 acaacaaaca tcgcaagacc gcagaagagt ggaacatccc ggacgccgaa ggctacgcta
    13021 actctcgtac catcaaagag tgctacgatg ccttccagtc gctggagtac gaagtaaaca
    13081 ccctgcacac cgccaacggt cagacgccgt ttgtaacctt tggttttggt ctgggcacca
    13141 gcagggaatc gcgcctgatt caggaatcga tcctgcgtaa ccgtatcgca ggcctcggta
    13201 aaaaccgtaa aactgcggtg ttcccgaaac tggtgtttgc gattcgcgat ggcctgaacc
    13261 ataaaaaagg cgatccgaac tacgacatca aacagctggc gctggagtgc gcaagcaagc
    13321 gcatgtatcc ggatatcctg aactacgatc aggtagtgaa agtcaccggt tcgtttaaaa
    13381 ccccgatggg ctgccgcagc ttcctcggcg tgtgggaaaa tgaaaacggc gagcagatcc
    13441 acgatggtcg taacaacctc ggcgtgatca gcctgaacct gccgcgtatt gctctggaag
    13501 caaaaggcga tgaagccacc ttctggaagc tgctggatga acgtctggtg ctggcacgta
    13561 aggcactgat gacccgtatc gctcgtctcg aaggcgtgaa agcgcgcgtc gccccgatcc
    13621 tctatatgga aggtgcttgt ggcgtacgtc tgaatgctga cgatgatgtt tctgaaatct
    13681 tcaaaaacgg tcgtgcgtct atttcgctgg gttacatcgg catccacgaa accattaacg
    13741 cgctgttcgg cggcgagcat gtttacgaca acgagcagct tcgcgcgaaa ggtatcgcca
    13801 ttgttgaacg tctgcgtcag gcagtggatc agtggaaaga agaaacgggt tatggtttca
    13861 gtctctacag cacgccgagt gaaaacctgt gcgaccgctt ctgccgtctc gatactgctg
    13921 agtttggcgt ggtgccgggc gtgaccgata aaggttacta caccaacagt ttccaccttg
    13981 atgtggagaa gaaggtgaac ccgtacgaca agatcgactt tgaagcgcct tacccgccgc
    14041 tggcgaacgg tggtttcatt tgctacggcg agtatccaaa cattcagcac aacctgaaag
    14101 cgctggaaga tgtctgggat tacagctatc agcatgtacc gtattacggc accaatacac
    14161 cgattgatga gtgctacgag tgtggcttta ccggtgagtt cgagtgcacc agcaaaggct
    14221 tcacttgccc gaaatgtggt aaccatgacg cctcccgtgt gtcggtaact cgccgcgtgt
    14281 gcggatattt aggtagcccg gatgcgcgtc cgtttaacgc cggtaagcag gaagaagtta
    14341 agcgccgcgt taaacatttg gggaatgggc agataggtta atcttcactc tgcgttaagc
    14401 aaatgccgga tgcggcgtaa acgccttatc cggcctacat ttggcaacgg ctgtaggcct
    14461 gataagacgc gacagcgtcg catcaggcat tgattaccgg atgcagccta cacgcggact
    14521 gataagccag gcgaaataat gaattatcat cagtactatc ctgtcgacat cgtcaacggc
    14581 cccggcactc gctgcaccct gtttgtctcc gggtgtgttc atgaatgccc cggttgctat
    14641 aacaaaagca catggcgggt aaattctggt cagccattta ccaaagcaat ggaagacaag
    14701 atcattaaag atctgaatga cactcgtatc aaacgccagg ggatttccct ctccggcggc
    14761 gatccgctgc atccgcaaaa cgtgccggat attctgaaac tggtaaaacg catccgcgcc
    14821 gagtgtccgg gtaaagacat ctgggtgtgg acaggctata aactcgacga actcaacgct
    14881 gcgcaaatgc aggttgttga tttgattaac gtgctggtcg acggcaaatt tgtgcaggat
    14941 ttaaaagacc cttccctgat ctggcgcggc agcagcaatc aggtggtgca tcatttgcga
    15001 tgataagttc cctgttacct gagtgcaggc aacagggaaa tgaggaatta acgatacttc
    15061 tggtgatagg cgttgcgggt cgttttcagt tgctctgcag cagcctgcgc ttcttttact
    15121 ttaccttcat ttgccagctt cagcgcgtcg tcaatctgac cgaccagaat gtcgaaaccg
    15181 tggcggaaat ctttcatttc cgggctgtcc ggtgatttat cttcgagctt cggcggcgtt
    15241 gctttttgcg catccagcgc tgcggcgcgc atcttcgtta acgcgtcttt gacttgcgtc
    15301 gcgttatcgg ctttttcgac cacttttaaa ttgtcgttga gggtttccat attgtcttca
    15361 agatcagcgg caaacgacgc cgaactgaat accaacgagg agactgcaag aatagctaac
    15421 aggcttttac gcattgctca cttccttttt tatttacact tcatccttca cactgcctca
    15481 tcgacagcta cgctcattcg accgggtcac gtactacgag tacgctcccc ggctctcatt
    15541 cactttccgt ctcgatgcag tgcgaatgat tttgtgtaat tttttagttt atagcgcggc
    15601 aggtcgcgcc agttttttta ctgtccggcg attttcatct ccggcaacag cacagaacca
    15661 cactgtatat tactgcgtgt ttcaatatcg ttaccgacgg tgacaatatt gcgccacata
    15721 tcttttaaat tacctgcgat ggtgatttcg ctcaccggat actgaatttc gccattctct
    15781 acccagaagc ccgcagcacc acgggaataa tcaccggtaa tggcactcac gccctggccc
    15841 atcagctccg tcaccaccag cccggtgccc atctctttga gcatttgttc gaagcttagc
    15901 ccttgtccgg caatccgcca gttgtgaata ccgcccgcat gtccggtgct tttcagccca
    15961 agttttcgcg ccgaatagct ggtcagcagc cactgagtca ggatgccatc tttaataata
    16021 tcgcgacgct cggtgcgcac accttcgctg tcgaatggcg tcgacgccag ccctttcagc
    16081 agatgcggat gctcttcaat ggtcagccag tccggcagaa tttgtttacc cagcgaatcc
    16141 agcaggaagg tagatttacg ataaaccgat ccacccgcta tcgcccccac cagatgccca
    16201 aaaagcccag ttgccacttc attggcaaaa atgactggcg ctttcatggt ggagagttta
    16261 cgcggtaaca ggcgcgataa agtacggcga gcacagtcgg ccccaaccca ctctggcgtt
    16321 tgcagatcgc tcatcgcgcg cccaatggtg taggcgtaat cacgctccat atcgccattt
    16381 tcttcggcaa ttacacagct ggagagcgaa tgacgcgttg agcagtaacc ctgcaacatg
    16441 ccgtggctgt tgccaaaaac tttgacaccg tagtggctgt taaagctgcc accttcggta
    16501 ttggtgatac gtttatccgc ctgtaatgct gcctgttctg cgcgggccgc cagttcaatg
    16561 gcttcatccg gggaaacttc cgcagggtgg aacaagtcga gatctggtgc gtcaaaggcc
    16621 agcagctctt tgtctgccag gccggcacag ggatctggcg aggtataacg ggcaatatcc
    16681 agcgccgcct gtacagtacg ggcaatggcc tgcgggctta aatcggtgga tgatgcgcta
    16741 cctttgcggt tctggtgata aacagtgatc cccagcgcgc catcgctatt gaattcaaca
    16801 ttctccactt caccataacg cgtgcttacg ctaatgccgg tggtcttgct gacggcaact
    16861 tccgcaccgt ccgatttgcc tgaggccaac tccagcgcag tggaaactgc ttcttccaga
    16921 atcttgcgct gcgcttcaac ttgagagatt actttcattg caagtgccat aatgtagaga
    16981 gtttctctga agtctaacag agaaccgttt ttcagtgcgc atcttaactg gtaacattag
    17041 cctctttttt taaggagcct gagatgacta agcagcccga agactggctc gacgacgttc
    17101 ccggtgatga catcgaagac gaagacgatg aaattatctg ggtcagtaaa agtgaaatta
    17161 aacgtgatgc cgaggagcta aaacgccttg gcgcggaaat tgttgatctg gggaaaaacg
    17221 cgctggataa gatcccgtta gatgcggatc tgcgcgcggc tattgagctc gcccagcgta
    17281 ttaagatgga aggtcgccgc cgccagttgc agctcatcgg taagatgctg cgccagcgcg
    17341 acgtagagcc tattcgtcag gcgctggata agctgaaaaa ccgtcacaac cagcaggtgg
    17401 tgctgttcca taaactggaa aacctgcgcg atcgtctgat cgatcaaggt gatgacgcca
    17461 tcgctgaagt attgaatctg tggcccgatg ccgatcgtca gcaactgcgt acactgatcc
    17521 gcaacgcgaa gaaagagaaa gaagggaata agccgccgaa atccgcacgc cagattttcc
    17581 agtatctgcg cgaactggcg gagaatgaag ggtaattttc gttgttgatg tgagtatggt
    17641 ttgccggatg cggcgtaaac gccttatccg gcaatgctat atcaggctga agccgaatta
    17701 ctgcgcagct tccgccttct tcgccagacc atccagcagt ttctgatgga tcccaccaaa
    17761 accgccgttg ctcatcacca gaatatggtc gccaggctga gcggttttca ccaccatatc
    17821 tgccagcgta tccacatcgc cactccagtg tgcaggctga acgcaggctt ctgccacttc
    17881 tgccacctgc cacggaatat gcgccggttg cagcaggaag acttcatcgg cacgacctaa
    17941 tgaaggtgcc agatcgtctt tgcagatccc cattttcatg gtattcgagc gcggttccag
    18001 cacagcaata atgcgcgccg taccaccaac tttgccacgc agcgccgcca gcgttgccag
    18061 aatcgccgtc gggtgatggg caaaatcgtc atataccgtg acgccattcg cttcaccacg
    18121 caactccaga cggcgacgag cattaataaa cgaacccagc gcgttagcgg catctgccga
    18181 cgctacacca acatggcgag ccgctgcaat cgccatcagg ccattgtgca tattatgttc
    18241 gcctaccagc gaccatttca cttcgcccac tttttcgcca tccagcaaaa cttcccattc
    18301 ggaagcatcg gtggtcagct ttttcgcctg ccagtgaccc tgctcaccca ccagctcctg
    18361 ctcgctccag cagcccatcg ccatggtctg tttcaggttg atgtcatttt ccggccagat
    18421 aatacggccc tgccccggaa cgatacgcac cagatggtgg aactgtttct ggatcgcttt
    18481 caggtcgtca aagatatcgg cgtgatcgaa ctcaaggttg ttgaggatca gcgtacgcgg
    18541 gcagtaatgg acaaatttag agcgtttgtc gaagaaggcg cagtcgtact catccgcttc
    18601 gataacaaag aagtcgcttt cgcccagacg cgccgaaacc tcaaagttcc ccggcacacc
    18661 gccgatcaca aatcccggtt tgtaaccaca ctgttccaga atccaggtcg ccattcccgc
    18721 ggtggtggtt ttgccgtgtg taccggcaac ggccagcacc cagcggtcgc gcagcacaaa
    18781 atcgtgcagc cactgtggac ctgacatata agggatgttt ttttccagta ccgcttccac
    18841 acacggattt ccacgggtca tggcgttgcc aataatcacc agatccggct gcggctcgag
    18901 ctggctggca tcgtaaccct gaatcagctc aatgccttgc ttctcaagta aggtgctcat
    18961 cggcggatac acattggcgt ccgaacccgt tacttcatgg cctaactggc gcgccagcat
    19021 cgccagaccg cccataaatg tgccacaaat tcctaaaata tgaatgcgca tacgtcacta
    19081 tcctttttta atctgccgct cattttacgc atatgtcggg caagtgagaa acgcatttca
    19141 ggataatccg caatttgctg gcgcgattca cctgagcgca acattgagat tatttgttaa
    19201 gattgttgcg gtcgctttac tccataaaca ttgcagggaa agttttatga aaacgttagg
    19261 tgaatttatt gtcgaaaagc agcacgagtt ttctcatgct accggtgagc tcactgcttt
    19321 gctgtcggca ataaaactgg gcgccaagat tatccatcgc gatatcaaca aagcaggact
    19381 ggttgatatc ctgggtgcca gcggtgctga gaacgcgcag ggcgaggttc agcaaaaact
    19441 cgacttgttc gctaatgaaa aactgaaagc cgcactgaaa gcacgcgata tcgttgcggg
    19501 gattgcctct gaagaagaag atgagattgt cgtctttgaa ggctgtgaat acgcaaaata
    19561 cgtggtgctg atggaccccc tggatggctc gtccaacatc gatgttaacg tctctgtcgg
    19621 taccattttc tccatctacc gccgcgttac gcctgttggc acgccggtaa cggaagaaga
    19681 tttcctccag cctggtaaca aacaggttgc ggcaggttac gtggtatacg gctcctctac
    19741 catgctggtt tacaccaccg gatgcggtgt tcacgccttt acttacgatc cttcgctggg
    19801 cgttttctgc ctgtgccagg aacggatgcg cttcccggag aaaggcaaaa cctactccat
    19861 caacgaagga aactacatta agtttccgaa cggggtgaag aagtacatta aattctgcca
    19921 ggaagaagat aaatccacca atcgcccgta tacctcgcgt tatatcggtt cactggttgc
    19981 ggatttccac cgtaacctgc tgaaaggcgg tatttatctc tacccaagca ccgccagcca
    20041 cccggacggc aaactgcgtt tgctttatga gtgcaacccg atggcattcc tggcggaaca
    20101 agcgggcggt aaagcgagcg atggcaaaga gcgtattctg gatatcatcc cggaaaccct
    20161 gcaccagcgc cgttcgttct ttgtcggcaa cgaccatatg gttgaagatg tcgaacgctt
    20221 tatccgtgag ttcccggacg cgtaatctcc tgcgcctctc cacacggaga ggcgttttta
    20281 ttgctgcgca atgttgactc ttgtcactgg cgaactctga cgattctccc acagcaccgt
    20341 taatccacgc tgtaatgcta taaaaataaa caacaaaata ccgatggcga ttttcgtcca
    20401 ccaggaactc agcgtgccat caaagtttat gtaagtctga atcagtccct gaatcgccac
    20461 gccaaaaagc gtccctaata ccgttccaac gccaccgctc aaaagcgtac cgccaattac
    20521 cactgaggcg atagcatcca gctccacacc tacgcccgcc agcgcatatc cggcctgggt
    20581 ataaatcgag aagacaatcc ccgccagcgt tgccagtccg gtggagagca tataaatgcg
    20641 aatagtggtg ctgcgagtgg aaatccccat cagattcgcc gacgttgcgt tgccgccaat
    20701 ggcgtatacc tgattaccaa aacgggtacg atgcgcgagg aatatgccga taaccaccac
    20761 cgccaacatc agcagtccca tcgcacttaa gcgaccgccg ccagggattt tccacgcaag
    20821 gcttgagagc gtgtcataaa tgggatggtt tatcggaatc gactcttccg aaacgagata
    20881 gctgacgccg cgcaaaaaga acatccccgc aagcgtaatg atgaacgccg ggatcttcag
    20941 ggcgtcgatc agaagcccca taaatgcgcc gaaggcacag cccatcacca gcaccagcgg
    21001 aaacgccagc agcggcgaga ggccgaaatc gccaatcact tttgccagaa acacgccggt
    21061 aaaggcgatc accgaaccga cggagagatc gatcccaccg gagaggatca caaaggtcat
    21121 gccaacggca atgatcccaa gaaaggcgtt atcggtcagg atattgcaga tcactcttgt
    21181 ggaagcaaaa ccaggaaact gcgtcaggca gtagagatag cccagcacaa agacgccgat
    21241 ggtgatcata agcggcaagt tacgttttat cacggctacg cactccttta atcagactga
    21301 taaagcgttg cgactgaaca atcagcacac aaagcaccac caccgctttc accacctggt
    21361 tcatctctgg cggaaagccc gaaagcaaaa ttccggtatt cattccctga ataatcagcg
    21421 ccccaactcc cgaaagcagc aggttaaagc gaccgcccat cagcgatcca ccgccaatca
    21481 ccaccgcgag aatggcatcc agctccagcc ataatccggc gttgttggca tcggcaccgc
    21541 gaatatccgc cgccacgata atgcccgcaa tcgccgcaca tagcccgctc aacacgtagg
    21601 tgagcatgac gatgattcgt gtgttgactc cggcattttt tgccgcccga atgttgatac
    21661 caacggcttc gataaacatc cccagcgccg ttttgcgggt caacagccag aacaggagaa
    21721 gcgtcagcac cgcgataatg accggcgttg gcaggaacaa cagcgatccg ctgccaaacc
    21781 atgagagatc cggcgagtta aacgtgacga tctgcccggc ggtgatcagt tgcgccacgc
    21841 cgcgcccggc gaccatcagg atcagggtgg cgacaaacgg ctgaattttg aggatcgcta
    21901 ccagtatgcc gttccacaat cccgctagga tgccggtgcc cagggcgctt aacaaaacaa
    21961 tcggcaggct gaatcccgcg accgtcatcg cagccgttgt ggctccggcg atagccatca
    22021 ccgcccccac agagagatcg ataccaccgg tggcgatcac cagcgtcata ccaatcgcca
    22081 gtaacgcaac gggagctgca cggttaagaa tgtcgatagg gctaccgaac aaacgcccat
    22141 cctgaagcac cacctgccag aaatgcgggg ctaccaggct atcgaccagc agcaccagca
    22201 acagtgccgc cagctgcggc atccccgtcg gccagtgaaa acgccttttt ggcggcgtag
    22261 tgtcagggag agattgaggc atcacactgt tctccttacg ccgcaatggc gttcatgatc
    22321 gccggaacgg aaagctgtgc cagcgggatc tccgccacct gtttgcgatc gcgcatgatg
    22381 atcacccggt cggcatagcc caccagctct tccagttcgg aggagatcac cagcagcgcc
    22441 agaccgtcag cacacagcgt ttcaatcagg cggatgatct cggcgtgggc accaacatca
    22501 atgccacggg ttggctcatc aaggatcaga aattgcggtc gggtcagtag ccaacgtgaa
    22561 agcagcactt tttgctgatt gccaccggag agaaattcaa tcggttgttc agttgaaggt
    22621 gtgcgaatgc caagctggcg gataaagcgt tcggcaatct cttgctgctc tttgcgggaa
    22681 atgggtcgta gccagccgcg ctgggcctgg agagcgagga tgatattttc ccgcaccgag
    22741 gcggcggcga tgatgccatc ggttttcctg tcttccgggc agaatccgat gcccagtact
    22801 gaagcctgat gtggcgatcg cagggtttgc ggtttgcctt tgatcaacgc cgtgccgctg
    22861 tcagcaggtt tgataccgaa gatcacttcg gcggtttcgg tacgtccgga tcccagcaag
    22921 ccagccagac cgacgatctc gccggggcgc acttcgagat caaacggtgc tatcgttcct
    22981 tttttgccgt aatttttgaa cgcggcaacg ggtttgtcgc tcaacaatgt tcgcccggca
    23041 cgctgtagca cgtgggtatc cagctcgcgc cccagcatca tttttaccag ttcgatctgc
    23101 ggtagctcgc gcgtttcccg acagcctacg aaactgccgt tgcgtaagac agtgatccga
    23161 tcgctgacct gatagacctg atcgagaaag tgagtgacaa agatcaggct gacgccgcga
    23221 tcgcgcaact gacgcatcag gccaaataat agctccactt cctgggtgtc gaggctggcg
    23281 gtgggttcat cgaggatcag cactttggcg gagagatcga tagcccggca aatggcgacg
    23341 atttgctgca tcgcgactga aaagcggtta agcggttcgc gcacgtcgag gaagaaaccg
    23401 taagatgcca tcagttcggt ggcgcgcttt tccatctctt tacggcgtag aaggccgaaa
    23461 cgtttgggtt cgcgacctat gaatagatta tcagcaaccg acatattggg tagcaggttg
    23521 acttcctgat agacggtgcc aataccgagt tgttgtgcat gggcggtatt tttcggtgag
    23581 atagcctggc cttccagcca gatggtgccg cgatcggcgt ggtatacacc agttaatgct
    23641 ttgattagcg ttgattttcc cgccccgttt tcaccaagca gcgccatgat ttcgccacgg
    23701 cgcaggctga aatcaacgtt gtctaacgct ttgacgccgg ggaaaaattt acttaatcct
    23761 tcggtgcgga ggatctcctg gtgttggtcg gtcgtcatgg tttcccccgg tgtaaagcgt
    23821 gctcccagta ggcatgataa gacgcggtaa gcgtcgcatc aggcttcagt gcaccatcgc
    23881 cggatgcggc gcgagcgcct tatccggcct acgaatcggc atcaggcata gcaaccctca
    23941 ataccccata tttttcttct tctctaactc ttcttttgcg gtatcaggca ggtaaagggt
    24001 ggatttggtt aacgtcagct tttcaggcat ggtgccgtct tttttgtatt tctccagcgc
    24061 atcgaaagcg ggtcccgcca tgttcggcgt cagttcaaca ctggcgttcg cttcgccatc
    24121 catcatcgct ttgtagatgt ccggtacacc gtcaatggaa cccgtgagga tatctttgcc
    24181 cggtttcagg cccgcttctt taattgcctg aattgcacca atcaccatgt cgtcgttatg
    24241 ggcgtaaacc atgcagatgt ttttgccgtt gttttccgct ttgataaagc tctccatgac
    24301 ttctttgcct ttactgcggg tgaagtcacc tgactgcgag cggataattt tgatatttgg
    24361 cgcattctta atggcttcgg caaagccttt cttacggtca atggcgacgc tggccccaac
    24421 ggtgccctgc agctccacca cgttgcatgg tttgccattc acttctttta ccagccagtc
    24481 accgatcaac ttaccttcga ggatgttgtc ggcagtaacg gtggtcatat agagagattt
    24541 gtctttcaca tcgatggaac ggtcgagcaa gaagaccggg atttcggcat ctttcgcctc
    24601 ttttaatacc ggttcccaac ccgtcgcaac cactggagcg ataaagatcg catccacccc
    24661 ttgtgcaacg aaggaacgta ccgctttaat ctggttttcc tgcttttgct gaccatcggc
    24721 aattttcaac gtaattccgc gtttttcggc ctcacttttc gccacattgg tttctgcggc
    24781 gcgccagccg gattccgatc cgacctgcga aaatcctccg gttaatggag ctgccaacgc
    24841 catagacgac atggctgccg agactgcaga gactacaagt aagcgtttcc acatagggtt
    24901 gtcctcgtcg gggtaattat tattggtgaa aagatgttcg cgaaaaaact atagacaatt
    24961 cgttatgtaa cggattgcgt tacatcacac ttcaaaaaag taaataaaac gttaatcaca
    25021 agtttgtaat cgctttcatc tcactatgaa aaatgcggct acggttatgg attttcctgc
    25081 tctgtatacc gtcttaaaac tggcgaaaaa ggaaaatgaa gacgaaaaca agcaaagaca
    25141 ttcggcgcga gttggctata atactcggca cttgtttgcc acatattttt aaaggaaaca
    25201 gacatgagct tactcaacgt ccctgcgggt aaagatctgc cggaagacat ctacgttgtt
    25261 attgagatcc cggctaacgc agatccgatc aaatacgaaa tcgacaaaga gagcggcgca
    25321 ctgttcgttg accgcttcat gtccaccgcg atgttctatc cgtgcaacta cggttacatc
    25381 aaccacaccc tgtctctgga cggtgacccg gttgacgtac tggtcccgac tccgtacccg
    25441 ctgcagcctg gttctgtgat ccgttgccgt ccggttggcg ttctgaaaat gaccgacgaa
    25501 gccggtgaag atgcgaaact ggttgcggtt ccgcacagca agctgagcaa agaatacgat
    25561 cacattaaag acgtaaacga tctgcctgaa ctgctgaaag cgcaaatcgc tcacttcttc
    25621 gagcactaca aagacctcga aaaaggcaag tgggtgaaag ttgaaggttg ggaaaacgca
    25681 gaagccgcta aagctgaaat cgttgcttcc ttcgagcgcg caaagaataa ataagttctt
    25741 ctagcgcaat aaccctgaac gccgggcttc ggttagtaag ggttttttat gcccgcgata
    25801 aataaactct ctattccacc atcattattc tcagcggttg caaggcttga acggtaagaa
    25861 caagcaaacc cgaccaccat tttgctgttc atagccactt gctggaagtt agccgacctc
    25921 actcatactc accgttcaaa gcgtgccccc gttttacccc tgcacatttg tgaacgccag
    25981 cagagttttt tatgcgattg cagatatgga aagcggctcg gagagaaaag caaaagatgg
    26041 aaaagttaca aaaaaaaatc gcgagataca tcgcataaaa caacgccacc cgaaggtggc
    26101 gtgcttttat tattacttat ccctgtctaa ccagtcgccg ctttcaatta gctttaatcc
    26161 atcgacgggt cgttgataaa cgtacatcca tgcactcccg tacggcgtct gaatcaactg
    26221 gcgcgcgtat tcaccgcccc tggtgcgcaa ggcatcaagt tcggccagcg tggcgttgtc
    26281 aatacgataa acttcaccgt gtaccgttcc gttccccgga actgcgcctg gatagtggcc
    26341 caggctatac aactggtagt tatcgatact gaaatcgccc agtaactggg cattggtcat
    26401 ccagtgactg ttgccttgtt tgtggcgtaa actgccgtag acaaatattc gcattgctaa
    26461 aactcgaact gatagagcaa atccagtgcc tggtctacac cagacacggc ttccagatat
    26521 agcttaggca tcaggcgata acgtaacgtg agtgttgcta tagagtcaaa tatacccacg
    26581 ccgtatttca cttgcagacc tggcaataca tagccgctga ccactacctg ggaggagtcg
    26641 cctactccct gggtgtcgag cgctaaattg cttacgccaa acgtctcgcc gattttaccc
    26701 acaatctggc cactttgcgc aacccccaga ccaatcagca tcgaggtcat tgccgcactg
    26761 tcgctctgat cgctctccag cccttgtcca cgtagcaaat aagacaatgc agcttgttgc
    26821 gacatcgccg ggtcagagaa gatctccgct ttcggttcgt ccgccagacc agtgacgcga
    26881 actccggcaa ttacgtcgtc ttctgtagca tccgggttac gaatagcttc aatattaaga
    26941 tacggttgat ccggcggacc agagaacagt aactcaccct tacgcacaat cagatcctga
    27001 ccataggcat ggaagcgccc ttcagggatg ttgatctgcc cgttcaggcc cagcccttgt
    27061 ttgtcctgta cgacgttaag atcgcccgtc agccgcgctt tcaggccaaa ggcgtcaatg
    27121 cgcacattgt tgccaacgtg gacaatcagg ttactgttaa tcggaatcga cgccgttttc
    27181 ggctcttccg gttgcaggtt atcgttaagc atcaccacat cgctggagac gcctactgcg
    27241 ctttccggca gatcgtgcac cacgatgcgc gcccacggga catccacgcg accatcgagg
    27301 gtaaacaggt ttggtgtagc ctcgaataca acatctggcg atacatccat tcgtaccatc
    27361 ggcggcacgg tgatccgcac tttactgcct ttcgccgtta ctcgtgcccg ccagttttca
    27421 atttggctcc agtcggcatc accgttcagg tagatttcac cctgctgggt ccgtactgta
    27481 ccggcaagcg tcgagcgcat accgttaaag ttgaccgcaa gctggctcgg ctgcatatca
    27541 aacggcataa agttgccgtc gatatccaca cccgtaacct gaagctgacc aaacaactgc
    27601 gggctttgca catcaccacc cagacgcaag ttggcactca ccatccccgc tgctttttcc
    27661 ccacgggtaa agatggggtt tatcatcgca aggttgaagt tacggatatt gacgttgcca
    27721 ccaagattac ggcggccttg cggatcggtc acctgcacct gtccatcaaa ctggccgtta
    27781 ttggtcaggc ggatggtcca gcccaattcg gcacggttgt tacgcaattc cgccgtcaga
    27841 ttcagtgtct gaaacgccac cggcagcgcc gcatcgttga cggtttgcgt tacctgcacg
    27901 ttacgccccg aaagggtgat actgccctgc ggcagcccct ctttcgtggt gtcccaggca
    27961 acatccgctt tacccgtgaa gataccgctg gcctgagtgg tttctggcat aaacggtttc
    28021 agcatggcga ggtcgaagcg gttgagattc accaccgcac gcccttcggc ccccgcatcg
    28081 atagtttgcg gcacgcacag ttccgcattc gggttaagcc agcagtgtgg cccgatgctg
    28141 attttttgct ccttattgcg gtaatccagc gcaatatcgc gggtcagcga ccacgggccg
    28201 accggcgtct ggaaacgggt attgctaaga gttcccttcc agcgttcttc tttgcgatca
    28261 aaacttcctg ccagattaag ctgcccggag acaggctcgc cctgaatccg caactgtagc
    28321 tcgtgctgct tttcgctgcc tttggcattc agggtgacga ggttgatatt tacatccggc
    28381 tgcgaaattt gctcaacgcg tacgtcgagt ttcccggcga tctgatcggt ggatttaatg
    28441 tcgccttcca cgcgaacctg cgccacggaa agttcctgcc agcgcaggcc gcgcgcggtg
    28501 atatctgcca gtagttgtgg cgcttccacc gtgccgcgta ctttcaccag ccctttcgct
    28561 gtaccgccaa gccccggcag cgcgttatcc agccccggcg cgttgatggt ggcatcaaga
    28621 ttgagatctt ttacccccag ctcgcctttc acttcggcac tgtttggccc gagttccaga
    28681 tgaagcccag ggatcatcca ctgcatataa ctgttgcctt tcagcgtgcc gtcaacgttc
    28741 actttgttct gtttgacgtt accggtcagc ttcagttctg gcacctccat ctgccaggtg
    28801 ccgccgtaca ggctaccgcg ggttttaatc aagccattga gtttcgacgg ccactccggg
    28861 aactctttgg cggtgttaat gccattaagc gttagctcac cgcgccaact aatggcctgc
    28921 tgccagtcga gcaacgcctt gagttcagtt ttcccttcca gcgccgcgac ggtgagtttg
    28981 tcgagattga cctgctgttc attacctttg gcatcaaggg taatggtggc tggcgggatc
    29041 tcctgtccct tcactgccgt acgcatagag agcgtgtaat cggtcatttt gccggtaagt
    29101 ttcagtttca gatcatccgc ctgatactgc ttctcaccag tgaacggcca gtaaagctgt
    29161 ttgctgttca cttccacgtt gagtggcaat ccggcttccg ccagtcgcgt ctgggcgcgt
    29221 aaatccatat ccaccggacc ggaaaggtta acgcctatct ccagctgttc gcgcagcgcg
    29281 ccgcccactt tcagcttcac cttttcacct ttcaacggct ccacgttcag tgtgctgttg
    29341 aggatgatat ccaccggcca gttgtctgac agctgcgccg tgccgctggc gttgacgatc
    29401 ccctggttgg aatcgatatc cagggcgtcc agtttagtat tgccgtcaat gctgctcact
    29461 ttcagcagca tggtgcgcac ggtgatgtcc gtgtcgcccg tcacgcgcag ctgctcgccc
    29521 ttaaactctt caatgttcag gttaagcggc aaatgcacgt cggtcatttc cggcaatacc
    29581 gggcgagaaa agagatcttt cagcgtttcg ccgagcggct tttcatccgg ctgcggattt
    29641 tcaatcttcg gttcgacgac ctcttcctgc gccacttccg ccactttcgg cagagcaatc
    29701 agcaggcctt tcagcgacgt cggtttcagg gtcagggttt tctcctgcca gttcaggccg
    29761 gaggtgaagt ccatcaccga caccgtggtg tcatcaatct tgatgttgac gttatccagc
    29821 gccacccgtg tcagggtgat gggatacggc gtggagagat ccagcggacc gctatcttcc
    29881 tcttcttcaa cctgttcaga aggaggcatt tttttactgt cgatgttgac ctgaatgtct
    29941 ttcagcgcca ggtcattaat acaaacgcta ctgttccaca ggcactcaag accgaccgcc
    30001 agatgcagat tacccgcttt taccgccacg cctggctgct cataacgaac gtcagacaag
    30061 gtgagatcgc gccagccgcc ggtgacgttg ccaatatcca gtcctggcac ccagcgatcc
    30121 gccgctttaa atacaagatg caggccgctg gtggtgccca ccagaaacgc caccgatccc
    30181 agcaacagta agataacgat aaccacgccg aggctgattt ttttccataa actcataatt
    30241 ctggccccag accgatgtaa aactgtaacc cgtgttcgtc tttatccgcg accggtacgg
    30301 caaaatcgag tttgattggc ccgaccggcg attcccagcg tacgccgccc ccggtaccgg
    30361 ttttaaagtc gctgcggcga atatcgctta ccgcttcgcc actatcgaca aacaccgcgc
    30421 cccaccattt tccggtcacg ttgtactggt attccagcga tccggttatc agcttcgagg
    30481 cccctttcag gtcaccgttg gcgtatttcg gggcgataga tttatatttg tagccgcgaa
    30541 tactacgatc gcctccggcg aagaaacgca gatccggcgg tactttgtcg aaatcaccgg
    30601 tttcaatcca gcccagcgtg ccgcgtgtca caaaacgatg gcgatcgtac agtgtgcgga
    30661 tccagacgtt ctgcgcctgg aaaacggaga aatcaacatc tgaaccccag gccgtgttgg
    30721 agtagtcgat agagtagcgt tgcgagtcgc cccaggttgg catcaggcca ccacgagaac
    30781 gcgtgcggct aatcatcacc ccaggataaa acagcatcgt ggtgttggta atttcaccct
    30841 gggtaaagtg gtcgaggctc cagcgcaggt taatggcacg ctgccagtcg ctggagagat
    30901 cccagtagcg agaagccacc agcgtagtgg agtcagattc ggtatcgttc aggtcagtgc
    30961 gcttaaaacc gccctgcacc aaataatatt gttccagtgg attcttcagc agcggcattt
    31021 tatagctgaa gtcgaggatc tgttccggcg cggaaatact ggtactggtg gtcagactgt
    31081 gaccataaga gttcatccac ggctttttcc acgtcgcttt cacgcgcggt cccacgtccg
    31141 tagagtaacc gaccccggtt tcgatggtgt tttctgttcg cggcgaaacc acgcccgtca
    31201 atggtaatac tttcgtttcg cgcgctttat caaattgcgg tgccactacc accgaattaa
    31261 accagccggt agcagaaagt cggcggttca gttccgccag atctttcgat tcatactcat
    31321 cgccctcttt aaacggcacc agattttgca ggtattcatc gcggatttgt gatccttcaa
    31381 aggtcacatg cccaaagcgg taacgttcgc cactgttata atcaatatcc cagaaggctt
    31441 tatgcaggcc gagcgcaatg cccagctgcg ctttggtaaa ttcgctatcg aaataacctt
    31501 tacgcaacgc aatactggtt aaggactttt tgaaattttc ataatcgccc tggttcagta
    31561 ccgtaccaat agccgggcga gtatcgagca atttcaaata gtctttatcg gtccgcgcgc
    31621 cgccgcgcaa taccacatcg gtgccgccaa ttaacaccgg tacacctggc gtgactttgg
    31681 cgatcaatac ctgccgccct ttctttggcg gtggacggag atcaaattca atggtcggct
    31741 ggtaataacc cagcgctttc agaccttcac ggatggcatc atcgacgcgt gcgcgaaagc
    31801 gacggtctgg cgtcacttca tcactttcaa tcgtagaaag ctgcgcacga acgttctttt
    31861 ccagctgtcc cgataacccc tcgacctgta gacggacgtt cgcggcgacg gcagatccgc
    31921 ttaagcagag taagcttaca cagcataact gtcggatata gcgcacattt tctcctgaat
    31981 atcctttttt cctacccctg gaacgccgtt aaacggctta acaaaaatcc agtaatatgg
    32041 attaaaaagc agactaaacc ccaaatattt cttatgtttt actttagacc tattcacggt
    32101 ggttattgtg tgcaaatacg cctcttgtta caaccttaac cccaatgacc gattttcggg
    32161 agagcgacac catgagttta tttgataaaa agcatctggt ttcccccgcc gatgccctgc
    32221 ctggacgtaa caccccgatg cccgtagcca cgctgcatgc ggtcaacggt cactcaatga
    32281 ccaatgtacc tgacggaatg gagattgcca tttttgcgat gggttgtttc tggggtgtgg
    32341 agcgtctgtt ctggcagtta cccggcgttt acagcaccgc cgcaggctat accggcggct
    32401 atacgccaaa tccgacttat cgggaagtgt gctccggtga tacgggtcat gccgaagcgg
    32461 tacgcattgt ttacgatcct tccgtcatca gctatgagca gttgctacag gtattttggg
    32521 agaatcacga tcccgcccag ggaatgcgtc agggcaatga ccacggcacg cagtatcgtt
    32581 cagcgattta tccgctgacc cctgaacagg atgccgcagc tcgcgccagt ctggaacgtt
    32641 ttcaggcggc gatgcttgcc gccgatgacg atcgtcacat caccacggaa atcgctaacg
    32701 ccacaccgtt ttattatgcc gaagatgacc accagcaata tctgcataaa aacccatatg
    32761 gttactgtgg aattggcgga attggcgtct gtctgccgcc ggaagcatag cgttacgggt
    32821 acaaatgtag tttgttgata aagtgctctt tgtctatgcc tgatgcggct taaacgactt
    32881 accaggccta caaaatcgtc caaattcaat atattgcaag gactgcgtag gcctgatcgg
    32941 catagcgcat caggcagtat tgcatttatc agcagtctga attttaaccc tctggcgact
    33001 ttacagtacc ttacgctata ctagccactg aaaatgtcgg ttcactttct tcgaatcggc
    33061 tttcaatgtg tatttcacac aaattaatca actttccctt ccgaggatct ggcctgaaag
    33121 gtcggataag atatgttaaa cagtatttta gtcatactct gcttgatcgc tgtaagtgcg
    33181 ttcttctcga tgtccgagat ctcgcttgcc gcctcacgca aaatcaaact taaactgctg
    33241 gctgatgaag gcaatataaa tgcccaacgc gttctgaata tgcaggaaaa tcccggcatg
    33301 ttctttaccg tggtccaaat cggtctgaac gcagtggcga ttctcggcgg tatcgtcggt
    33361 gatgcggcat tttctccagc ttttcacagc ctgttctccc gctatatgtc ggcagagctc
    33421 tctgagcaac tgagctttat tctctctttc tcgttagtga ctggcatgtt tatcctgttt
    33481 gcggatttaa ccccgaaacg catcggtatg attgcgccag aagcggtggc tttgcgtatc
    33541 atcaacccga tgcgcttctg cctgtacgtt tgcaccccac tggtgtggtt cttcaacggc
    33601 ctggcgaaca tgatcttccg tattttcaaa ctgccaatgg tacgtaaaga tgacatcact
    33661 tctgatgaca tctacgcggt agtggaagcc ggtgcgctgg cgggcgtgtt acgtaaacag
    33721 gaacacgagc tgattgaaaa cgtctttgag ctggaatccc gtaccgttcc gtcttcaatg
    33781 acaccgcgtg aaaacgtgat ttggtttgat ctccacgaag atgagcaaag cctgaagaat
    33841 aaggtggcgg aacatccgca ctctaagttc ctcgtctgta atgaagatat tgaccacatc
    33901 atcggttatg tcgattctaa agacctgctg aaccgcgtgc tggctaacca aagcctggca
    33961 ctgaacagcg gcgtacaaat tcgcaacacg ctgattgtgc cggatacgtt aaccctttca
    34021 gaggcgttgg aaagttttaa aaccgcaggt gaagacttcg cggtgatcat gaacgagtac
    34081 gcgctggtgg tggggatcat caccctcaac gacgtgatga ccacgctgat gggcgatctg
    34141 gtcggtcagg ggctggaaga gcagattgtc gcccgtgatg agaactcatg gctgattgac
    34201 ggcggcactc cgattgacga cgtcatgcgc gtgctggata ttgacgaatt cccgcagtcg
    34261 ggcaactacg aaaccatcgg cggctttatg atgtttatgc tgcgtaagat cccgaaacgc
    34321 accgattcgg tgaaattcgc cggctacaaa tttgaagtgg tggatatcga taactaccgc
    34381 atcgaccagc tgctggtgac ccggatcgac agcaaggcca ccgccctttc gccaaaactg
    34441 cctgacgcta aagataaaga agaaagcgtc gcgtaacccc ccagaaacat caacggctcc
    34501 tgaatcagga gccgtcttat tactgcatag cactttttac tgcatagcac tttggttaag
    34561 ccatctctgt ttgcagacgc ataacctgac ggttaacttc ggacatcact gacaaatgca
    34621 gcttatcctt cacttttggg ataagaatct tacccttatc aaattcaaaa gcgccaacgt
    34681 ccttgatgta taaccgtcca cggaacagga tctttacgta cttcgccacc tgaagtgggt
    34741 tgtaccgttg gaaaattttc attgttatct cctgctgagt attacgcctg tgcggtgcca
    34801 caatcggccc aactattatg aggcgcaaat tttaatgctt agtgactata gactatccgg
    34861 gcaatgtttc caccgcgtat aacttttttt acctaaaggt tacaattatt cagaattatc
    34921 tttttaccga agcgcggtct tcagtataag cattcatttt tcatatgatt tgtgcgcttg
    34981 accgcaaact ggcatcacac ttgcgggaaa ttcgataaat agcacatatg attaaaactc
    35041 agacccaagt ggtcggatca cctgcatatc ataagaagga aacaccatga ccctacgcaa
    35101 gattctggca ctcacctgcc tgctgttgcc gatgatggct tccgcacatc agttcgaaac
    35161 cggtcagcga gtgccgccga ttggcattac cgatcggggc gagttggtgc ttgataaaga
    35221 tcagtttagc tacaaaacct ggaacagcgc gcagttagtg ggaaaagtgc gagtactgca
    35281 acatattgct ggtcgcacct ctgcaaaaga gaaaaacgcg acgctgattg aagcgattaa
    35341 atcagcgaag ttaccgcacg atcgttacca gaccaccact attgttaaca ccgacgacgc
    35401 gattccgggt tcagggatgt ttgtgcgcag cagtctggag agtaataaaa agctttatcc
    35461 ctggtcgcag tttattgtcg atagcaatgg cgtcgcgcgc ggtgcctggc agctggatga
    35521 agaaagttcc gctgtagtgg tgctggataa ggatggtcgc gtgcaatggg ccaaagacgg
    35581 ggcgctcact caggaagagg tgcagcaagt gatggacctg ctgcaaaagt tgctcaaata
    35641 atggcaacaa catagattct gcaaaaataa ttttccccta tcgttatcta tgggataggg
    35701 gaattaagtc tctgagttct tacgccgaac tcatactggt aacgctagtt gctctttttt
    35761 aaacgcctca atggtcgact tatatttctt gcaaatcttt tctttggtaa tattactatc
    35821 tgcggccaga ttcgcactta ctatcgcatc cacttgttcg gctaattcca cttgttggca
    35881 acctgcatta accagaaaat tataaataag ggagtaatct tcatcctcca aaacaacaga
    35941 ggaaccatta cgaaaacgtt catcccctcc atagatttta agcgcaaagc ccaaagattc
    36001 tatgactatg gcaaaattat catcaattat tttttcgtga atataccatc catcgcattc
    36061 tttaattcca cctcctggta tttggagttc aaatttaccc cgttttaatt tgcttactat
    36121 atcctccaat aaggcataac ctaaagacac gtcaccaatc gcctggtgaa tataaccttt
    36181 aatccttttc ggtatgtcag actgtttttt ctgcagaata tcacaggcat acaatcttct
    36241 gttttgttct aacggaaact cgcggttttt gaaatccatg aatgagttat actcatttat
    36301 tgttatggga atttcttcgt attcaaatcc actacctcgc aatgtcattg tatattcggc
    36361 accattatct ttaaggctga aactgataag gaacttatcc atggcgttaa cagttccatt
    36421 ttttgatgaa agtacctgat aactattttt atcaatctga gttgcgcaac gaaagaactc
    36481 cctcaaagtt aacaggctat cataattcga aatccaatct tttttcttaa atgcatgctg
    36541 gatattgtta aaaaaattgt ttgaatgtgt ttttgtatac gatgacttta caaataactc
    36601 caccgcacca ataaaattat tgtgtctgat cctgggtaac atatgacaac cttattttat
    36661 ttggttattt tatgtcgcca ttaaatatct cattagagaa gtaaactatc aaaaaacgct
    36721 cattattaaa atgtaactta ttagcaattg aatttttgca ctttaaatac aatctctttt
    36781 tatttataac tttttaggtt gggagaaatc aggatggcag aatcaggaaa tacactgttt
    36841 ctgccatctg aatttagtaa atagacactc tgaaccccgg attcaggaac gactcgcgcg
    36901 gagtgtaatc cagcggttta ccctgccagt cgtgaacgtg cgctccggca gctgcagcca
    36961 cagcatgtcc agcggcggtg tcccaaatat tcgttggtcc gaagcgcggg tacagctgcg
    37021 cctgtccttc cgccaccagg cagaatttca gcgaagagcc gatggacgtg gtctgatgtt
    37081 cgccaagctg ttgcagatac tctttcagct ccgcatctgc atgggaacgg ctgataacca
    37141 ccagcggcgg gcgcgcatcg cggacctgaa tctgcttgcg cacaccgcac tcttctttcc
    37201 aggccttgcc ttctgccgcg ctgtacatta cgttcattac cggcgcatac accacaccta
    37261 aaatcggttt gccatggtca atgagcgcaa tgttaacggt gaattcgcca ttacgtttaa
    37321 taaactcttt agtaccatcc agcgggtcta ccagccagta acgctgccag tgctgacgga
    37381 cttcccaacc gggaggatct tcttcagaaa ggaccggaat atccggtgtc agcgtacgta
    37441 aaccgtccat gataacggtg tgtgcggcaa tatccgctgc cgttaccgga gaattgtccg
    37501 ctttgctgac gacgtccatc ggtttcgtcc cgtcgtagac ctgcataatg gcatcgcctg
    37561 cattccgtgc aagctggcat acttgatcta acatttctcc acctcgtctc tgtgagcggt
    37621 gttaacttat tgttttactt ataccctatc gttaatgaat gcgccaactg tgatagtgtc
    37681 atcattttca aagcgtaaaa ttgtggcatt cttcactgtt ctataagtaa gacgtttatt
    37741 ctcccttttc tttcgtattc ccgatgataa aaggatgtcc ctgatgatta agtttagcgc
    37801 aacgctcctg gccacgctga ttgccgccag tgtgaatgca gcgacggtcg atctgcgtat
    37861 catggaaacc actgatctgc atagcaacat gatggatttc gattattaca aagacaccgc
    37921 cacggaaaaa ttcggactgg tacgtacggc aagcctgatt aacgatgccc gcaatgaagt
    37981 gaaaaacagc gtactggtcg ataacggcga tttgattcag gggagtccgc tggcagatta
    38041 catgtcggcg aaaggattaa aaacaggtga tgttcatccg gtttataagg cgctgaatac
    38101 gctggattat acggtcggta cactcggcaa tcatgaattt aactacggtc tggattacct
    38161 gaaaaatgcg ctggcgggag cgaaattccc ttatgtaaat gccaacgtca ttgacgccag
    38221 aaccaaacag ccaatgttta caccgtattt aattaaagat accgaagtgg tcgataaaga
    38281 cggaaaaaaa cagacgctga agattggcta tattggcgtc gtgccgccgc aaatcatggg
    38341 ctgggataaa gctaatttat ccgggaaagt gacggtgaat gatattaccg aaaccgtgcg
    38401 caaatacgtg cctgaaatgc gcgagaaagg tgccgatgtc gttgtcgttc tggcacattc
    38461 cgggctgtct gccgatccgt ataaagtgat ggcggaaaac tcagtttatt acctcagtga
    38521 aattccgggc gttaacgcca ttatgtttgg tcatgctcac gccgttttcc cgggtaaaga
    38581 ttttgctgat atcgaagggg ctgatatcac caaaggcacg ctgaatggtg ttccggcggt
    38641 aatgccgggc atgtggggcg atcatcttgg ggtggtcgac ttacaactca gtaatgacag
    38701 cggtaaatgg caggtgacgc aggcgaaagc ggaagctcgg ccgatttacg acatcgccaa
    38761 taaaaaatcc ctcgcggcgg aagacagcaa gctggtagaa acactcaaag ccgatcacga
    38821 tgccacacgc cagttcgtca gcaagccaat cggtaaatct gccgacaata tgtatagcta
    38881 tctggcgctg gtgcaggacg atccgaccgt gcaagtcgtg aacaacgcgc aaaaagcgta
    38941 tgtcgaacat tacattcagg gcgatccgga tctggcaaaa ctgccggtgc tttcagctgc
    39001 cgcaccgttt aaagttggtg gtcgcaaaaa tgacccggca agctatgtgg aggtggaaaa
    39061 aggccagttg accttccgta atgccgccga tctttatctc taccccaata cgctgattgt
    39121 agtgaaagcc agcggtaaag aggtgaaaga gtggctggag tgctccgccg gacagtttaa
    39181 ccagattgat cctaacagca cgaaaccaca gtcactcatc aactgggatg gtttccgcac
    39241 ttataacttt gatgttattg atggtgtgaa ttatcagatt gatgttaccc agcccgcccg
    39301 ttatgacggc gagtgccaga tgattaatac caatgcggaa aggattaaga acctgacctt
    39361 taatggcaag ccgattgatc cgaacgccat gttcctcgtt gccaccaata actatcgcgc
    39421 ttacggcggc aaatttgccg gtacgggcga cagccatatc gcttttgctt caccggatga
    39481 gaaccgctcg gtgctggcag cgtggattgc tgatgagtcg aaacgtgcgg gggaaattca
    39541 cccggcggca gataacaact ggcgtttagc accgatagct gccgataaga aactggatat
    39601 ccgtttcgaa acttccccgt cagataaagc cgcagcgttt attaaagaga aagggcagta
    39661 tccgatgaat aaagtcgcga ccgatgatat cgggtttgcg atttatcagg tggatttgag
    39721 taagtaaaac acttcttttt cggcctataa atcatcaatc gcatccggca tttattggcg
    39781 gatgcgatgc tggcgcatct tatccgccct acaagtcata caccgtaggc cgcgcatccg
    39841 gcagttatgc cgcacgttca tcccgcaccg ccaacacctc gggcaaattc aactcaatcc
    39901 agtccgccag tgcagcaacc ttttcgctca cctgctcgcc cagcggcgtg aggctgtatt
    39961 caacatgcgg tggcaccacc ggatacgcga tacggttaag aaaaccatcc tgttctaacg
    40021 cctgtaacga ctgcgcaagc atcttttcgc tcaccccgcc gattttgcgc cgcaggtcgc
    40081 taaagcgatg agtaccttcg cgtagcgcca ccagaatcaa caccccccaa cggctggtga
    40141 cgtgtttcaa cacctcgcgc gacgggcact gttccgcaaa gagattaccc tctttcagtt
    40201 gttgcgacag gctaacctga ctcatttcat acttaccttt ttgtacgtac ttactaaaag
    40261 taagtttagg tgttagcgta tttaaacaca agacaaaacg atggagactt cccatgatcg
    40321 ctattactgg tgccactggc caacttggtc actatattat tgaatccttg atgaaaacgg
    40381 ttcctgccag ccaaatagtg gctatcgttc gtaatccggc aaaagcccag gctctagcag
    40441 cacaaggcat taccgtgcgt caggctgact acggcgatga agccgcactg acatctgcac
    40501 ttcagggagt ggaaaaacta ctgctgatct cttccagcga agtgggtcaa cgtgccccgc
    40561 agcatcgtaa tgttattaat gccgcaaagg cggctggcgt gaaatttatc gcttatacca
    40621 gcctgctaca tgcagatacc tccccgctcg gcctcgccga tgagcacatt gagacggaga
    40681 aaatgctggc tgattctggc atcgtttaca ccctgctgcg caacggctgg tacagcgaaa
    40741 actacctcgc cagcgccccg gcagcactgg aacacggcgt atttatcggt gcggcgggcg
    40801 atggcaaaat cgcctcagca acgcgggcag attatgcggc agctgcggca cgcgtgatta
    40861 gcgaagccgg tcacgaaggc aaggtttacg aactggcggg cgatagtgcc tggacgttga
    40921 cacagttagc ggcagagctg accaaacaga gcggcaaaca ggttacctat caaaatctga
    40981 gcgaagccga tttcgccgcg gcactgaaaa gcgtcggact gcccgacgga ctggcggata
    41041 tgctggcgga ttctgacgtt ggcgcatcga aaggtggtct gtttgataac agcaaaacgc
    41101 ttagcaaatt gattggccgc ccaacgacaa cgttagccga aagcgtaagc catcttttta
    41161 atgttaataa ctagttaatt aaagtggcat cctcccgcat cctctctgat aatgacggga
    41221 tgccgggagc aatcatgtct gcttcctgaa ctttcttctg acagaccaat ggatgccagt
    41281 aatgattagc ggcgtgctgt acgccctgtt agcagggttg atgtgggggc ttatttttgt
    41341 cgggccgttg atcgtaccgg aatacccggc gatgttgcag tcgatggggc gttatctggc
    41401 gttagggtta attgcgctgc ccattgcctg gctgggacgc gtgcgtctgc gtcagttggc
    41461 gcgtcgcgac tggcttaccg ccttgatgct caccatgatg ggcaacctta tctattactt
    41521 ctgccttgcc agtgccattc aacgtactgg cgcgcctgtt tccacgatga ttatcggcac
    41581 cctgccggtg gtgattcccg tttttgccaa tctgctttat agccagcgcg acggcaaact
    41641 cgcatgggga aaactcgccc cggcactggt ttgtattggc atcggcctgg cgtgtgtgaa
    41701 tattgctgag ttaaaccacg gactccccga ttttgactgg gcacgttata cctctggcat
    41761 cgtgctggcg ttagtttccg tggtctgctg ggcgtggtat gccctgcgca acgcccgctg
    41821 gctgcgggaa aatcccgaca aacatccgat gatgtgggca acggcgcagg cgctggtcac
    41881 actgccggtt tctctcatcg gctatctcgt cgcctgttac tggctgaata tacaaacgcc
    41941 ggacttctcc ttaccttttg gcccccgtcc gctggtgttt attagtctga tggttgcgat
    42001 agccgtgctt tgctcatggg ttggcgcact ctgctggaac gtcgccagcc agcgattacc
    42061 gacagtgatt ctcgggccgc tgattgtttt cgaaacgctg gcaggtttgc tgtacacctt
    42121 tttactccgc cagcaaatgc cgccgctaat gacgctgagc ggtatcgcgc tgttagtgat
    42181 tggcgtggtc attgcggtca gagcaaaacc agaaaaacct ttaactgaat ctgtctcaga
    42241 aagttgacac gctggcagtg agttaaataa gcctctgcta cgtaagggtt atagcttttg
    42301 ccttaaagat gcatttaaaa tacatcttat attattaaga atgaggtatc agctatggct
    42361 tatcgcgacc aacctttagg tgaactggcg ctctctattc ctcgcgcttc agctctgttt
    42421 cgtaaatatg atatggatta ctgctgtggc ggtaagcaaa cgctggcgcg cgcggcggca
    42481 cgtaaagaac tggatgttga ggtcattgaa gctgaactgg caaagcttgc tgaacaaccg
    42541 attgagaaag actggcgtag cgccccgctg gcagaaatca ttgaccatat catcgtgcgc
    42601 taccacgatc gtcaccgcga gcaactgccg gagctgatcc tgcaagcgac taaagtcgag
    42661 cgcgttcacg ccgacaaacc gagcgtgcca aaagggctga caaaatacct gactatgctg
    42721 catgaagagc tatccagcca catgatgaaa gaagagcaga tcctcttccc gatgatcaaa
    42781 caaggcatgg gcagccaggc aatgggacca atcagcgtga tggaaagcga gcacgatgaa
    42841 gcgggcgaac tgctggaagt gattaaacac accaccaata acgtcacacc gccgccggaa
    42901 gcctgcacca cctggaaagc gatgtataac ggtattaatg aactgattga tgacctgatg
    42961 gatcacatca gcctggaaaa caatgtactg ttcccacgcg cgctggcggg tgagtaatct
    43021 aaaagctgga tggcattgcg ccatccagca tgataatgcg gttatttccg cagttcagca
    43081 gcccgcttct taccaataaa caaccagccc agccccagtg cgataaacca cagcggggtg
    43141 accaacagcg cctggcgagt gtcatcttcc agtgtcagca acacgaccac gaacacaaag
    43201 aacgccatac atacccagca catcagcttg ccgagcggca tcttgtagat cgacttctca
    43261 tgcagatgag gacgctgttt gcggtaaacc aggtacgagc aaaggataat tgtccagacg
    43321 aacataaaca gaatcgcgga aacggttgta atcatcgtga acgcgccaat cacgctagga
    43381 ttcacataca acatcaccac gccgccgagc agacagatac acgagaaggt caaccctttc
    43441 gcgggtactg cgcgcttaga aagtttagcg aacgctttcg gtgccacacc ttcctgcgcc
    43501 agaccaaaca gcatacggct ggtagagaag acgccgctgt tagcggaaga cgccgcagag
    43561 gtcagcacca caaagttgat cacgctggca gccgcaggca aacctaccaa cacaaacagt
    43621 tcaacaaacg ggctcttctc cgggactacc gaactccacg gcgtcacgga cataatcaca
    43681 atcagcgcga agacgtagaa cataatgata cggatcggaa tggagttaat cgcgcgtggc
    43741 agtgatttct ccggatcttt ggtttccgca gcggttgtac ctaccagctc aatccctacg
    43801 aaagcgaaaa ccgctatctg gaatccggca aagaagccac ttaaaccttt cgggaaccag
    43861 ccgccatcat tccacaaatg cgcgaatgac gcttccacac cagtcggtga ctgaaagtgc
    43921 atcgccacca taaccaggcc gacgacaatc agcgacacaa tggcgacgat tttgatcatc
    43981 gcaaaccaga actccatctc accgaacatt ttcacggtgg cgagattgag cgtcagcagc
    44041 agcactatca ccgccagcga ggcgacccag tcggagagat cggggaacca gaactgagca
    44101 taagcagtga tcgccaccac gtctgccata ccggttacaa cccagcagaa ccagtaagtc
    44161 cagccggtga aatatcctgc ccacggcccg agtaaatcgg aagcgaagtc actaaaagat
    44221 ttgtattcca gattcgaaag cagcaattcc cccattgccc gcatcacgaa aaagagcata
    44281 aaaccgatga tcatataaac gaaaatgatc gacggcccgg caaggctaat cgttttgccg
    44341 gaccccataa acaaccccgt accaatggca ccgccaatgg caataagctg aatatgtcgg
    44401 tttgtgagat tgcgccgtag cgactgttca gccggagcct gatcatcggc aacgactttt
    44461 acctgatcta ccatgttttt ttcttcctgt acctgtctgt gttgttcagg ctctacggcc
    44521 tttagtcagt ctatgatacg acgaatctgt atcgggactc atcgatatta ggtaacaata
    44581 cgcgggatga atactaagat ttagatctaa tgttaatttt atgtttaaag tgagtgtcat
    44641 atcacggtga taacgcgaaa cagctcacaa aaatacacgc ttacggggtc tttgcaacac
    44701 aaaatagcgc cgtttggcaa actgaagggt ttattgctga atgcctgctc ccctctggtt
    44761 cgaggggaac agaatgcgct gcttagagga tttccagcag ttcgacttca aacaccaggg
    44821 tgctgaacgg agggatggat gcgcctgcgc cgcgctcgcc atatgccagt tcctgcggga
    44881 tagtcagttc ccatttagaa cctaccggca tcagagtcag tgcttcaatc cagccaggga
    44941 tcacgccatt aaccgggaat tcagcgggtt caccacgagc aacggagctg tcaaacacgg
    45001 tgccgtcgat cagtttaccg gtgtaatgaa cacgaacgcg gtcggtacgt gccggaattg
    45061 cgccttcacc ctggttgatc acgcggaatt gcaggccaga ttcggtgcta ttcacacctt
    45121 cttttttggc gttttcttcc aggtatttca caccttcagc agccatcgcc tggaaacgct
    45181 gacgacgaac ggcatcggcg cgttcgtgga tttcacgcag cgcgcgatgc accacatcaa
    45241 ccggcacagc cggatgtttg ccttccagcg catcggcaat acctgcaacc agtgcttctg
    45301 gcagcagccc ttccaggcca gattcactca gttgttgccc tacctgcaaa ccaatgccgt
    45361 agcttgcttg cgcttcgatg gtgtcaaaag ttggggtggt catcgtattt cctttcttag
    45421 ggtataaaag tagcgggcag catatcagcc acacttcttt gggtaaaact ttgtcgcagg
    45481 aatgatgaca aatcccgggg aaaaagaaac aatagcaata tcccgtatga atataataaa
    45541 ggcgggttta ccacggacat catagcgtta gcttattcat aggctatgat tgaggaacaa
    45601 gacgcggagc aggaggaaac catgcccggg cgctttgaac taaaaccaac cctggagaaa
    45661 gtctggcacg cgccggacaa ttttcgcttc atggacccgc tgccgccgat gcatcgtcgg
    45721 gggatcatta ttgccgccat tgtattggtg gtcggatttc tgcttccatc tgatgatacg
    45781 cccaacgcgc cggtggtgac gcgtgaagcg cagttagata ttcagtcgca atcacaaccg
    45841 ccgacagaag aacagctgcg ggcgcaatta gtcacaccac aaaatgatcc tgatcaggtg
    45901 gctccggtcg ctccagaacc tatacaggaa ggccagccag aagaacagcc gcaaactacc
    45961 caaacgcaac cattccagcc agatagcgga attgataatc aatggcgctc ttaccgtgtc
    46021 gaacccggta aaacgatggc gcagctgttc cgcgaccacg gactgcccgc taccgatgtc
    46081 tatgcaatgg cgcaggtaga aggcgcgggt aagccgctga gtaatttgca aaatgggcag
    46141 atggtgaaaa tccgccagaa cgccagcggc gtggtgacgg gcttaaccat tgatacgggg
    46201 aataatcaac aggtcctgtt tacccgccag ccggatggca gttttattcg tgcgcggtag
    46261 atgaagggtc aaccaaaagc cagctaagga ttgtctcttt gatgacctta cgtctggctt
    46321 ccagtgcctg gggtgaaacc atactgatgt gataaaccag gccaagcgta tgctggttga
    46381 tcaaataata tcccccaagc gcagcaatat taatattcac ctgcaatggg tcgatatctg
    46441 gcttaaaaat attgtgtttc ttcccttcat ccaataagga ttccattaac tgcaagtgcg
    46501 catggttaat ttctaagaga cgcagtgatt ttgcataatg cacaccttta ctttgatttt
    46561 cactatgcac aatttttaaa aaccaagggt
//
LOCUS       DAJVQG010000010        46343 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJVQG010000010 DAJVQG010000000
VERSION     DAJVQG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46343
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.010"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..673)
                     /locus_tag="OQM83_000555"
     CDS             complement(<1..673)
                     /locus_tag="OQM83_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HCS1518907.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(695..2707)
                     /gene="hyfR"
                     /locus_tag="OQM83_000556"
     CDS             complement(695..2707)
                     /gene="hyfR"
                     /locus_tag="OQM83_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCS1518908.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2737..3150)
                     /gene="hyfJ"
                     /locus_tag="OQM83_000557"
     CDS             complement(2737..3150)
                     /gene="hyfJ"
                     /locus_tag="OQM83_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCS1518909.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3143..3901)
                     /gene="hyfI"
                     /locus_tag="OQM83_000558"
     CDS             complement(3143..3901)
                     /gene="hyfI"
                     /locus_tag="OQM83_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCS1518910.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3898..4437)
                     /gene="hyfH"
                     /locus_tag="OQM83_000559"
     CDS             complement(3898..4437)
                     /gene="hyfH"
                     /locus_tag="OQM83_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCS1518911.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4447..6162)
                     /gene="hyfG"
                     /locus_tag="OQM83_000560"
     CDS             complement(4447..6162)
                     /gene="hyfG"
                     /locus_tag="OQM83_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HCS1518912.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDNWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6152..7732)
                     /gene="hyfF"
                     /locus_tag="OQM83_000561"
     CDS             complement(6152..7732)
                     /gene="hyfF"
                     /locus_tag="OQM83_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCS1518913.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7737..8387)
                     /gene="hyfE"
                     /locus_tag="OQM83_000562"
     CDS             complement(7737..8387)
                     /gene="hyfE"
                     /locus_tag="OQM83_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCS1518914.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8399..9751)
                     /gene="hyfD"
                     /locus_tag="OQM83_000563"
     CDS             complement(8399..9751)
                     /gene="hyfD"
                     /locus_tag="OQM83_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCS1518915.1"
                     /translation="MGVLFAALTTLCMLSLISAFYQADKVAVTLTLVNVGDVALFGLV
                     IDRMSTLILFVVVFLGLLVTIYSTGYLTDKNREHPHNGTNRYYAFLLVFIGAMAGLVL
                     SSTLLGQLLFFEITGGCSWALISYYQSDKAQLSALKALLITHIGSLGLYLAAATLFLQ
                     TGTFALSAMSELHGDARYLVYGGILFAAWGKSAQLPMQAWLPDAMEAPTPISAYLHAA
                     SMVKVGVYIFARAIIDGGNIPHVIGGVGMVMALVTILYGFLMYLPQQDMKRLLAWSTI
                     TQLGWMFFGLSLSIFGSRLALEGSIAYIVNHAFAKSLFFLVAGALSYSCGTRLLPRLR
                     GVLHTLPLPSVGFCVAALAITGVPPFNGFFSKFPLFAVGFALSVEYWILLPAMILLMI
                     ESVASFAWFIRWFGRVVPGKPSEAVADAAPLPGSMRLVLIVLIVMSLISSVIAATWLQ
                     "
     gene            complement(9727..9837)
                     /locus_tag="OQM83_000564"
     CDS             complement(9727..9837)
                     /locus_tag="OQM83_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HCS1518916.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(9854..10801)
                     /gene="hyfC"
                     /locus_tag="OQM83_000565"
     CDS             complement(9854..10801)
                     /gene="hyfC"
                     /locus_tag="OQM83_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCS1518917.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10812..12830)
                     /gene="hyfB"
                     /locus_tag="OQM83_000566"
     CDS             complement(10812..12830)
                     /gene="hyfB"
                     /locus_tag="OQM83_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HCS1518918.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12830..13447)
                     /gene="hyfA"
                     /locus_tag="OQM83_000567"
     CDS             complement(12830..13447)
                     /gene="hyfA"
                     /locus_tag="OQM83_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCS1518919.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13700..14170)
                     /gene="bcp"
                     /locus_tag="OQM83_000568"
     CDS             complement(13700..14170)
                     /gene="bcp"
                     /locus_tag="OQM83_000568"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCS1518920.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14170..14742)
                     /locus_tag="OQM83_000569"
     CDS             complement(14170..14742)
                     /locus_tag="OQM83_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCS1518921.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14888..15766
                     /gene="dapA"
                     /locus_tag="OQM83_000570"
     CDS             14888..15766
                     /gene="dapA"
                     /locus_tag="OQM83_000570"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCS1518922.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15783..16817
                     /gene="bamC"
                     /locus_tag="OQM83_000571"
     CDS             15783..16817
                     /gene="bamC"
                     /locus_tag="OQM83_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCS1518923.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17030..17743
                     /gene="purC"
                     /locus_tag="OQM83_000572"
     CDS             17030..17743
                     /gene="purC"
                     /locus_tag="OQM83_000572"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCS1518924.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17911..18759
                     /locus_tag="OQM83_000573"
     CDS             17911..18759
                     /locus_tag="OQM83_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCS1518925.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18774..20789
                     /gene="tmcA"
                     /locus_tag="OQM83_000574"
     CDS             18774..20789
                     /gene="tmcA"
                     /locus_tag="OQM83_000574"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCS1518926.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20863..21561
                     /gene="ypfH"
                     /locus_tag="OQM83_000575"
     CDS             20863..21561
                     /gene="ypfH"
                     /locus_tag="OQM83_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1518927.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21671..21871)
                     /locus_tag="OQM83_000576"
     CDS             complement(21671..21871)
                     /locus_tag="OQM83_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCS1518928.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21899..23026)
                     /gene="dapE"
                     /locus_tag="OQM83_000577"
     CDS             complement(21899..23026)
                     /gene="dapE"
                     /locus_tag="OQM83_000577"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCS1518929.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23030..23386)
                     /locus_tag="OQM83_000578"
     CDS             complement(23030..23386)
                     /locus_tag="OQM83_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCS1518930.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            23768..23827
                     /gene="ypfM"
                     /locus_tag="OQM83_000579"
     CDS             23768..23827
                     /gene="ypfM"
                     /locus_tag="OQM83_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCS1518931.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(23926..27039)
                     /gene="acrD"
                     /locus_tag="OQM83_000580"
     CDS             complement(23926..27039)
                     /gene="acrD"
                     /locus_tag="OQM83_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCS1518932.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(27203..28903)
                     /gene="narQ"
                     /locus_tag="OQM83_000581"
     CDS             complement(27203..28903)
                     /gene="narQ"
                     /locus_tag="OQM83_000581"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HCS1518933.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSPPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            29109..31088
                     /gene="aegA"
                     /locus_tag="OQM83_000582"
     CDS             29109..31088
                     /gene="aegA"
                     /locus_tag="OQM83_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCS1518934.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMVFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            31156..31731
                     /gene="nudK"
                     /locus_tag="OQM83_000583"
     CDS             31156..31731
                     /gene="nudK"
                     /locus_tag="OQM83_000583"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCS1518935.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            31857..32900
                     /locus_tag="OQM83_000584"
     CDS             31857..32900
                     /locus_tag="OQM83_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCS1518936.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(32996..34999)
                     /gene="tkt"
                     /locus_tag="OQM83_000585"
     CDS             complement(32996..34999)
                     /gene="tkt"
                     /locus_tag="OQM83_000585"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCS1518937.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(35019..35969)
                     /gene="tal"
                     /locus_tag="OQM83_000586"
     CDS             complement(35019..35969)
                     /gene="tal"
                     /locus_tag="OQM83_000586"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCS1518938.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            36258..38537
                     /gene="maeB"
                     /locus_tag="OQM83_000587"
     CDS             36258..38537
                     /gene="maeB"
                     /locus_tag="OQM83_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCS1518939.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            38830..39165
                     /gene="eutS"
                     /locus_tag="OQM83_000588"
     CDS             38830..39165
                     /gene="eutS"
                     /locus_tag="OQM83_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCS1518940.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGVIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            39178..39657
                     /gene="eutP"
                     /locus_tag="OQM83_000589"
     CDS             39178..39657
                     /gene="eutP"
                     /locus_tag="OQM83_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCS1518941.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            39632..40333
                     /gene="eutQ"
                     /locus_tag="OQM83_000590"
     CDS             39632..40333
                     /gene="eutQ"
                     /locus_tag="OQM83_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HCS1518942.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            40330..41133
                     /gene="eutT"
                     /locus_tag="OQM83_000591"
     CDS             40330..41133
                     /gene="eutT"
                     /locus_tag="OQM83_000591"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HCS1518943.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            41130..42146
                     /gene="pta"
                     /locus_tag="OQM83_000592"
     CDS             41130..42146
                     /gene="pta"
                     /locus_tag="OQM83_000592"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCS1518944.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            42185..42478
                     /gene="eutM"
                     /locus_tag="OQM83_000593"
     CDS             42185..42478
                     /gene="eutM"
                     /locus_tag="OQM83_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HCS1518945.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            42585..42872
                     /gene="eutN"
                     /locus_tag="OQM83_000594"
     CDS             42585..42872
                     /gene="eutN"
                     /locus_tag="OQM83_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HCS1518946.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            42884..44287
                     /locus_tag="OQM83_000595"
     CDS             42884..44287
                     /locus_tag="OQM83_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HCS1518947.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            44298..45134
                     /gene="eutJ"
                     /locus_tag="OQM83_000596"
     CDS             44298..45134
                     /gene="eutJ"
                     /locus_tag="OQM83_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HCS1518948.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            45124..46311
                     /gene="eutG"
                     /locus_tag="OQM83_000597"
     CDS             45124..46311
                     /gene="eutG"
                     /locus_tag="OQM83_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HCS1518949.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
BASE COUNT        11255 a        12197 c        12611 g        10280 t
ORIGIN      
        1 aatggcgaat ggcaatggcg aacggaatca caaacaaatt ggcgatacag tgctcaaacc
       61 cactggcgac aaacagggtg atgggcaaaa tcatggcgac gattttgtcg cataatgaac
      121 gcccgcaata actcatccac agcgccagac aaaccatcag attgcacata atgccgaggc
      181 tgacagattc agtaaatgta tgatgcattt tgccctcggc gcagtgtaaa accgcgactc
      241 cccacatcgc gttttcactc atcaccagcc cggaaaacca gattaacaaa ctgaaacagg
      301 caatacctgc cagattaccg caggccacca gaagtgcgtt aatcagccaa gttcgccaac
      361 taataacgcc ccgacttttt gccatcaccg tcattaccga cgaggtgaac aggctggtgc
      421 cacaaaccgc cagcaaaata aagccgagtg taaagcaaag gccgcccacc agatgggtta
      481 atgcctgtga agacggggca tcggcgataa cggaaaggta aaaagtaaat ccaatcgcca
      541 taaacacgcc agcggacatc gccattaaaa agaccgacac tttgctgcgg gcaattttat
      601 gggcagtaat ccgttcagcg attgccgctt ttctggcgct caactgcaag tcgaaagaga
      661 gtttgtttct catgcatact cctgcaaaag cagattacaa cacctcgcga accgagagcc
      721 ccagacgctg cattcgtgac agcagcgtgg tgcgcttcat ccctaatcgt gtcgccgcgc
      781 cacggggccc ggcaacaatg ccattggttt ctcgcaatac ctgaacaatg cgctgacgtt
      841 cttcttcgtc attctctggc gtcgtcgggt gcagcaactg cgccattgaa ctgcgaagcg
      901 ctgaatcttc atttagcgtc ggcagtaaat ggctttgtcg gacattaaga tgtaaattca
      961 gactgttacc gcgagtcagc agtaccgctc gctcaatcac gttttccagc tcgcgcacgt
     1021 tgcccggcca gtcccacgac atcaactggc gtagcgcctc ggtcgggatg gcgtcaattg
     1081 aacgattcat atggcgcgcc attttttgcg tgaaatgctt tgctaaaaga gggatatctt
     1141 ccggacggtc gcgtagcggc ggcaattcca gtgggaagac attcaggcga taaaagagat
     1201 cgctgcgaaa ctggcgatct tcaaccattt gccacaaatc acggttggtg gcggcaatga
     1261 ctctgacatt caccgggatc gttctactcc cgccgagacg ctcaatctcc cgctcctgca
     1321 atacgcgcag cagtttaggc tgaagttcta acggcagatc gccaatttca tcaagaaaca
     1381 acgtgccgcc atcggcaatt tcaaaacgac cacgatgggt attaatcgca ccagtaaacg
     1441 cccctttgtc atgaccgaat aactcacttt ccagaagact ggcggggatg gcagcgcagt
     1501 tgattttgac cagcggcttg tcgcgtcgcg ggctaagttg atggatcgct ctggcgatca
     1561 cctctttgcc ggttccggtt tcaccacaaa tcaacactgt actgtcgctc ttcgccacaa
     1621 tatctacctg ctggagcagg tcttccattg cctggctttg atagataatg tcaccgacgc
     1681 ccagattcga aaggagctgc tcgctaagct ggtggttttc ttgctgcaaa ctttcctgca
     1741 aatcggtcat gctacgccag gcatcggcat tgccaacggc aatagcgatg cgatcggcta
     1801 tgtgttgtag tagctgacag ttttcctcac taaagagagt ggaagaggta tgcgccagca
     1861 acaatgcacc cggtgtatgg ttgccaaagg taagcggtat aaggagcgca gattcgcagc
     1921 cgttagatgc aagtaagagt aataacgggt cgcgttgcca gagaaacaga tcgtctgcac
     1981 ggtgcgtcaa ggtcggttgt cgggtttgta gcgtttgtgt cagcaataca ctgtcgccag
     2041 gcatattgcg tggcagacac gcacaatgtg aggcagaaag atcgctgcac cacaggctaa
     2101 acttctcgtt ctttcgatga tcgcccagta ccatactgac tgaagccaga ccgaaaaaat
     2161 gatggatctc acgagcgacg tcagcgatca gatcgtcgag atcaagatgt gacagcactg
     2221 cattagtaat atcaaccagt acccgatgat gatcgcgatc tttctccagc aaacgcagcg
     2281 ccacctgctg ttcttcatca ttgagccatg attgcagaac cggggtgatt atccgcgcca
     2341 gccagttaaa cagtgtgcaa tcctggggag taaaacggca gccgtgggtg cgaataaagc
     2401 gtagctccgc cattccacta ctgccgagaa ggacggcgtg acgcgaataa tcgaccatct
     2461 cgccctgata ctctgttgtt gccgtcacat cgctgccatc atcatggtag cggtaataga
     2521 agttgctttt gagaacatgc agttcaataa gttgtaccgg gctgaacggc ggcggcagcg
     2581 ggatgaaggc gcgtaataaa gacgccttac cgtgtttctg cgctaaccgc tccgcgagca
     2641 ttccgtttac cgcttcaatt gttattcctt gcggcggggc aaacatcgcc tcgtctgaca
     2701 tagccatcaa cagtccttat taatgagaaa tccttctcac gcaatttttc tagccatcag
     2761 atagatggcc ggttcctgca caatttcatc caacagatga atgaattgaa tgctacgcgc
     2821 ctgccacaca gacgtcgcgt catccgccag tggcgcgaat gcccgggtca acagggcggt
     2881 gtggctggcg tcaataacaa tctcaccaaa agtcatcacc cccagcatct tacgtcgggc
     2941 ttgctcctct tcgaccagtg caagccaatc ttcgtattcc gtcagtgggc aggggaaggc
     3001 gacattcaga caatcaatca cgccaacgtg atgaccgata gccagcgagt aatacattac
     3061 ctgagagctg tgttccggca tctcgtcgct actggcgaca aacttttttc gcagcgtcca
     3121 gaaaacaatt tccccgcact cttcagtcat gtaacccccg gagtacgcga aagagttgct
     3181 gaacgatact attgagacgt ggatcgtcgg catcgcgcaa ccaggtgtta acccgatttc
     3241 ctgtaggatc gtcgcttaaa tggcgcagga gccgatcgca aatttctcgc ccctgacgat
     3301 agcccgccag ccgccgcgct tctcgctcaa tggcgatacg ctgtgatggc gggatctgcg
     3361 gccacaacgg ttgcatagtc accccggtgg gatcgcgata atccaccgcg tgaatcttct
     3421 gttgcagcaa accgagcgcc acggcgaaac cgtgaatggt ggcggccggt gttggcgggc
     3481 agccggggat ccaaacatca atggggacaa tggtgtcgct gccgccccag acgctgtaga
     3541 gatcgtggaa aataccgccg ccgacaccgc acgcgccgta ggaaacacaa attttatgat
     3601 cgggggcaga ctcatacgcc cgaagtgcag gcatacgcat cgcccgggtg actgcgcctg
     3661 taaataacaa aatatcggcg tgacgcggtg atgaaataac cttaatgcca aaacgttctg
     3721 cgtcgaatac tggtgtgatg gcggcaaaaa tttcgatttc acaggcgttg cagccgccgc
     3781 aatcgacgcg ataaacgtaa gccgagcgac ggatatcctg tagcaaatgc tgcttcatct
     3841 tttgcgtttg ctcgtccagc gtgatgggct ggctgacatg ttgtgtaagc actggactca
     3901 tagctgctcc ttagccacca gcggtacatc tgtatcgtcg ttgagcagcg tcgcgcgttg
     3961 tttgcactcc gggcagacgc tcgcttgcgc ccataacatt tcgcggtttt gtggcgcatt
     4021 ttgttgctgt gccaacaatt cagcagccag tgcgacggtt ttttgcgggg caaacgggcg
     4081 ttcgcaacgg ctgcaacgtt gtagatggaa cgtcgcgcgg gtatagagat cggctttatt
     4141 ggtgacggtc agttcaaagt tattggtaag ctggatggct ctggtcgggc acacttcttc
     4201 acaacgtccg cagtaaatac aacgcctcag atagagctgc caggtgcgcg aattttgctg
     4261 gtcgtcggtc tggatagtca gcgcatttgc cggacaagca caggcgccgc aggcaataca
     4321 ttggctgggc atcaggtccg gtttcccgcg aaagcctggg ctgacctcca gtggcgcgaa
     4381 gggatatttc accgtcgcgg ttccggcgcg cataatagtt ttcagtaact tcagcatctt
     4441 ctgtccttac ttcagcggcg aacggttacg atcaatgccg tagcgttcga tctctttata
     4501 cggcacggtt tttgactggc gcttgcgcac atctaccagc gtcacacggt cggtacagga
     4561 gtagcaggga tcaaggctac cgataatcag cggtgcgtca gaaacggtat tgccgcgcag
     4621 catgtaacgc aacaccggcc agttggcgta ggtggcggca cggcagcgcc agcggaacaa
     4681 tttttggtta tcaccgagca tgctccagtg cacgtcttca ccgcgtggcg cttcaacaaa
     4741 gcccagcgcg aatgcgtgag gtttatagct aaagccttcg gtcagcagtg gggtatccgg
     4801 catgttgtcg agggcaaatt ccagcattgc cagcgaatca aacgtctctt tgacacggac
     4861 catcacgcgg gagaaaacat cgccgccggt aaaggtaaac agtgtttttg gaatattgcc
     4921 gtagtcggcg taggggtgat caaagcgcaa atcacgggca aaaccactgc cgcggatcag
     4981 cggccctaca gggctataat cacgggcgat ttgtcggtcg agaatgccaa tgccctgagt
     5041 gcgttgttcc atattcggcg tggcaagcag catctctacc agctccgaca cgtcggcgcg
     5101 catctcgcgc accagtttca gcgtttgcag acgttgctct ttgagaatat cgcggcgaac
     5161 accaccaatc agattcagac cgtaggtttt acgcgacccg gtcagcaatt ccgccattgt
     5221 catcgacttt tcccgcacgc ggaaaaattg cataaagccg gtatcaaatc caacaaaatg
     5281 gcaggagagg ccaaggttga gcaaatgact gtgcagacgt tcgacttcca gcagaatcga
     5341 gcgaatagta tgcgctcgtt gcggcacctc aatccccagt gcattttcaa ccgagttggt
     5401 ataggccaca ctgtgggcaa aaccgcaaat cccacacacg cgatccgata agaaggtcac
     5461 ttcgttgtag cccatccgcg tttctgccag tttctccatg ccgcgatgga cataaaacag
     5521 gcggtaatca gcatcgacaa tttgctcgcc atccacaaac aagcggaagt gacccggttc
     5581 atcggaggtg atatgcagcg ggccaatagg gatcacccgc gcatcgctat tgccctcatt
     5641 gataaacgga tacgtttcgg tatcagtcgt cggttcaggg cgcagtcgat aatccatcgc
     5701 atctttgcgc agcggatgca tatcttccgg ccagttatcg ggcaacacca ggcgacgctg
     5761 atccggcagg ccaaccggaa tcagcccgta catatcgcga atttctcgct cgccccagac
     5821 cgcggcaggg acgcgcggtg tgactgacgg aaactcccga ctgtcggcat cgaccagtgc
     5881 cttcaccacg atccagcatt tttcggcccc ttccattgaa agggcataat aaaccgcgta
     5941 atgaccgtta agtgtccgct cgtcgttgcc aaacaggact ggaagccagc catcatgttg
     6001 ataataaaga taatgtacaa cgtcaggcag cagattgatt ttcaccgtaa tggtgacctg
     6061 ttcaggcgtt tgtcgctctt catccagcac cgcgccgggg aactgcgttt tcagggcggc
     6121 gagaatcgct tcgccacgat ttgacgatga attaacgttc actgtgtttc tccgaaacag
     6181 atgcggtgcc tgaaggcaaa aaatcatgcc aggtgctacg ttgtgcaggc aggtcgtgcg
     6241 tccctgagag gactatagtg gaagcgcccg ccaggatcct gatgacaggt tgtggaatat
     6301 gcgttcccat cgccagcatc atgaccagca gaatcaccat tggcgaggtg gtcaaccagc
     6361 cgagatcacc ccggttaacg gcctgcggcg gtttcgccat taacacccgc gctgccatcc
     6421 gtaccaggcc cgccagcacc agcgttaaca gcaataacag caggacgata atcagcaggt
     6481 gattacgtgc cagtccggcg gtaatggtca taaattcgct aagaaaaatg ttgaagggcg
     6541 gcatccctgc cagcgccagc gcaccgccgc caaacagcac ggcggtaaat ggcatgattt
     6601 tgagcatccc acagacgacg ttgagatcgc gcgtgccgta cttgagcagt acattgccgg
     6661 aaccgcagaa cagcagcgtt tttgccagac tgtggtttaa gatgtgcagc agcgcggcaa
     6721 aaattcccag cggcccgcca atgcctagcg ccaccgcgac cagccccatg ttctccacgc
     6781 tggagtacgc cagcagacgc ttaatgtccc gctgtaccag aatgaaaaat gccgccacgg
     6841 caaccgacaa catgccgaag atgagcaaca accggttggg gaaatcgctg ccgatggctt
     6901 ggcaaataat gatgtaatag cgaatcagca ccaacagcgc gcagttcagc aatacggcgg
     6961 agagcagggc gctgaccgga ctcggcgctt cactgtgagc atccggcagc caggcgtgca
     7021 tgggaaatag cccggttttg gtaccaaagc caattagcac aaacacaaag gccaacagca
     7081 ttaatgttgg gtcaagcaag gacgattgct taagaacctc gctccagaat atcgccattt
     7141 ctgcctgcgg cataacgctg gcggcgttgg cgtataccag cacggtaccg aacagaccaa
     7201 aagcgacacc aacagtacaa ataatgatgt acttccatgc tgattccagc gatgaacgct
     7261 gaccgtaaat gcctaccaga aacgccgagc ttaaggtggt ggcttcgatc gccgcccaca
     7321 tcacaatcag gttattgctg gtaacaacca gcagcatggt gaacaaaaac agatggaaga
     7381 aaccgtagta atcgcacagc gtaacgggtg aaagctcgcc gtgtgccact tcatgacgca
     7441 tgtagccaat cgagtaaaca ccggtgagaa agccaatcac accaagaatg gcgaggaaca
     7501 aaccgcccag accatcaata tgaagccaca gtcccgcagc gaatatttct cctgcatcag
     7561 cggcagtttg gaccacccag agtgccagaa tcagcagcag tgtgatccct aagctatgta
     7621 atacagtcac cgtacaagtc gcagagagcc cccgtttccg gcaggcaaaa cagagcagcg
     7681 aaaaaagcag cggcgtgagc aggagtaaag cgaacatcac agaataactc atctcattat
     7741 cccttcagcg cggtcaagtt gttcacgtcc agcgtgccgt gggtacgcca tatttttctt
     7801 gccagtaaca ccatcacaat gacggcgaag atggcgtcgg tagcgatacc tatttccacc
     7861 agttccggtg ctcgccaggc aagaagcgcc agcaccagat gggagccgtt ttccatcagg
     7921 cagtaaccaa aaatttgccg caggatattg cgctggctga caatgcacag caggccaagc
     7981 agaaaatgac ctaacgctac cgccagcgcc ggtttcagcc cggtagccat cggtagcttc
     8041 acgggctgaa cgacaaatgc gcaaagcagg acaattaacg ccgcgagcag tgccatcatt
     8101 gccggaccga ataacgcttt ttccgggatg ttctggggaa tatttcgtgc agcgtaagtc
     8161 atgattaacg gtaccagcag cactttggtg ataaaggcgc tggcggacca gatcagcagt
     8221 tgctctgcgg cgaacaggca cgagagagtg gcgaaaatag acaccagcac cagcgactgg
     8281 caggcgtaaa atccgcatga caggcgatag cttttgacgc taatcacaaa cagcgatgtc
     8341 agcatcatca gtcccgccag attatttacg atcatagaac cggtcattca tcatctcctt
     8401 actgcaacca ggtcgcggcg attacgctgg aaatcagcga catcacaatc agtacaatca
     8461 acaccaggcg cattgaccct ggcagcggtg cggcatcggc gacggcctcg ctcggtttgc
     8521 ccggcacgac gcgaccaaac cagcgaataa accaggcgaa actggcgacc gattcaatca
     8581 tcagcagaat catggcgggc agcaggatcc agtactccac tgacaacgca aaaccgacgg
     8641 caaacagcgg gaatttactg aagaagccgt tgaacggcgg cacaccggta atcgccagcg
     8701 ctgccacgca gaaacccacg cttggcaacg gcagggtgtg caatacgcca cgcagacgcg
     8761 gcaacaagcg cgtgccgcag ctgtaactca gcgcacctgc tacaaggaaa aacaggcttt
     8821 tagcgaacgc gtggttgacg atgtaggcga tgctaccctc cagcgccagc cgcgagccga
     8881 agatggagag cgacaagccg aagaacatcc agccaagttg agtgatggtc gaccaggcca
     8941 gcaaccgctt catatcctgc tgtggcaaat acatcagaaa gccataaaga atggtgacca
     9001 gcgccatgac catgccaacg ccgccaatca catgcgggat attgccgccg tcgataatgg
     9061 cgcgggcaaa aatgtaaacg cccactttca ccatcgatgc ggcgtggaga taggcactga
     9121 tcggtgttgg cgcttccatt gcatccggta gccacgcttg catcggtagc tgggccgatt
     9181 tcccccacgc ggcaaacagg atgccgccat aaaccagata acgtgcgtcg ccgtgtaact
     9241 cgctcatcgc gctaagcgca aacgttccgg tctgcaaaaa cagcgtggcg gcggcaagat
     9301 acaaccccaa cgagccgata tgagtgataa gtaacgcttt tagtgctgaa agctgtgctt
     9361 tatcgctctg gtaataactg atcaacgccc aggagcagcc gcccgtaatt tcaaaaaaca
     9421 acaactgacc gagcagcgtc gaggagagta ccagtcccgc catcgcgccg ataaacacca
     9481 gcaaaaatgc gtaataacga ttcgtgccgt tatgcgggtg ttcgcgattt ttatccgtca
     9541 gataacccgt ggagtagatc gtgaccagca aaccgagaaa caccaccaca aacagaatca
     9601 gcgtactcat gcgatcaatg accaagccaa acaatgccac atcccccacg ttgaccaacg
     9661 tcaacgtgac ggcaacttta tcggcctgat aaaacgcgga gatcagcgac aacatgcaca
     9721 gcgtggtcag tgcggcgaac aaaaccccca ttcggcggcc cgacgttgtg gcgaaaacga
     9781 aacgaccagt gcgccgataa aaggcagcat taacgtcgtc agagcaagat tttccatatt
     9841 ccgtcagtgc tccttacaga ccggttaacc agaagaccca tgcaagcgca gcaaggctga
     9901 agccaagcca ggtcacatgg tgaatgagta aaaaacgccc gcgtgccagc gtgttttctg
     9961 cgattgaggc cagtacaaaa atcagcaaaa ccttgagcag cgtaacgaca agtgaagtca
    10021 gcaggcaggc gagagaaagt tcttgcgcgc gcccaaaggg cagaaacagg gccacaaaca
    10081 gtgctgccat cacgacctgt ttcagcccca gcccccattt cgctagcgcc agcccggcac
    10141 cggaatattc ggtcagcggg ccttcctgta attcctgttc tgcttcagca acatcaaagg
    10201 gaatttttcc catctcaatg aagcaggcaa aaccacaggc cagtaacgcc agtacggtgg
    10261 ttagcggcga gttccagccc gttgccagcg tattgctgat catctcgata tgcgtggaac
    10321 ctgctatcag cgccaatacc agcagtgaga gaatgagcat gggttcgacc agaatgccga
    10381 gcgtcaactc gcgactggca ccgactcccg caaacgggct tccggtatcc agccctgaaa
    10441 gagcaaagaa aaaacgaaac agggcaagaa gatagataag ggtgatcaga tcgccgccgc
    10501 ccgcaaaagg ggaaacggta ataaatagtg gtagggccat cgccagcacc agcatgctgc
    10561 tgattaatac ccacggcatc aggcggaaca tcagacctga agatgtcggc gcatcttcct
    10621 ggcgtttaaa cagtttgtgg atatcgcgat aatcctgcca gatccccggc ccgcggcggg
    10681 agtgcatacg cgcgcgtatc tgccgggaaa tacccgtaaa aagtggggtt agcatcagca
    10741 gaatcacggc ctgtgctaac gcaaaaatga ccagatatcc gtcgcaaaga gtttgtctca
    10801 tggtgatttc cttagacggc aatagcgatt agcagcacaa ccagtgcggc gaccacatac
    10861 aggcaataga gacgaaagtc gccgctttgc agatgctgta tttctttggc cagccgttgg
    10921 gtggcgctca cgatggggcg gatcacccgc tcatcccaga agggttctgt gctctgagcc
    10981 ctggcggtga cgtgcgcaag acctttattt agcctcagcg tagggtcgag ctgttgtcgt
    11041 agacgaaata gcgcagaaaa gaccacacgc agcggctgca tcacgccatt gcctgacggg
    11101 gccatcgcat tttcccagcc gtagccgcat gcccaggcat ctcctgtgcg acgaaacgct
    11161 gcgcggcgcg aacgacacca cagccagtaa aggccaggca gcaaaggcat cgccagtagt
    11221 aacagcaaca gtaatgaagg agtgacccgt gtatgaaacg tgccgggtac aagtgctatt
    11281 ccgctggcga cagtgacggg aggggtattg gtaaacgcat gggcaatgtg cattatcttc
    11341 ggtgccagcc aactggcact aagcgcaatt aatacgcaga gtgccgcgag cagtagcatg
    11401 gcgacgatca tcgtatttgg cacttcctgt gcctcttcag cgtgtgtgct gcgcggcgca
    11461 ccacagaaag taataccgta cattttgacg aagcacatta ctgccagccc accagtgact
    11521 gccagcatta ccatagcaat aggacccgca agttgtagcg ctacggcttc cacacgactt
    11581 agtgagaata gcgactgcca ggtgtaccat tcgctgataa aaccattcag cggaggaatg
    11641 gctgatatcg ccaggcaacc aatcaggcat gctgcggctg tccacggcat ccgtttcgcc
    11701 agtgccccca ttttttccat gtcgtgggta tgcaaacgcg aaataatcgc acccgcgccg
    11761 agaaatagca gccctttgaa aagcgcatgg ttgagcagat gaaacagtgc gccgagcagt
    11821 ccaaccacgg tgagcagcgg gtcgtgcagt gacagaccga ccatcgccac accgaccgcc
    11881 agcaaaataa tgccgacgtt ttcgacggta ctccaggcca gcagccgttt gatatcctgt
    11941 tccgccagtg catacagcac gcccaggagc gcggagattg cgccgatcgc catcaccaga
    12001 atgccccacc acagcggcaa acccgtttgc gccagcagat ccatcgccac tttcaggatg
    12061 ccgaaaatac cgattttgac cattacgcca gacatcaacg ctgaagcgtg cgatggtgcg
    12121 gcagggtgag cgcgcggcaa ccagctgtgc aacggcatca tcccggcttt cgcgccaaaa
    12181 ccgaaaaagg ccagcaggaa caccgccgac gccagccccg gagaaagtga aagcgtgcgg
    12241 aaactggcaa aatcgaggct gccgctttcg cgccccatca gcaagaaggc gatcattatc
    12301 agcaccgatc cggcgtgggc gataaaaaag tagagcatgc cagcgttgat cgacgtttta
    12361 tcctgcctgg caatgaccag aaaccaggaa gacagcgaca tcatttcaaa cagcacgatg
    12421 aaccaaaaag cgttgtccat caccagcagg gcaaccatcg atgcgatgaa aatattcata
    12481 aagaagccca tcgccgccgc gcctttgccc tcgtattcgc gcatataagt caatgaatag
    12541 agagaacaaa ccaccaccag caaggagata accagcacca taaatgccga gaggctatcc
    12601 attcgcaggg ttaaatcggc aaattcaaaa ggcgtggcaa aacgggcgac taaagttacg
    12661 cctgcatgta attgcgtaat tccgctgatg atgccaatca cgccgcccac cagcgatgtg
    12721 atgccggaaa gcttaatagc cagtctgtcc agaccgagta aaaacagcga agccagactg
    12781 gcaaacagat agaggatcag cgaccaggtt aataattgca gggcatccat tagcgttgct
    12841 cctgagtgag ggaaagggga tcctccccgc cattggcaaa ccagcttgcg gcaaggcgct
    12901 gtttttcttt catctggcgt tgcaggctat cgtcggtgat cagccgtaac gcctgattcg
    12961 ggcaagcgcg aacgcaggcc ggaccttctg gcaagaaatc acacaggtcg catttcaccg
    13021 cgacggtctg gacacccggt tcccagcgca gcaacgcatg ttggggcggc acgttttctt
    13081 cgccgtcttt gagtgagccc tcagcctgaa aaacatattg cgcaggggca ttcaccggac
    13141 ggcttcctga agcgctgatt gcgccaaatg ggcagaccac ggcgcaaagc ttgcagccaa
    13201 tacagaggct ttcgttgagt tggatcgcat catccctctg agagatggca ttgaccgggc
    13261 agacctgcag gcaaggggct tcctcacagt gatgacacac gacaggggca gtgagtgttg
    13321 acgtcttcgc cagggccagg cgcgggtgtt gctgtaaacc ttgcgtttta tgcacgtccg
    13381 aacaggcagc gagacaggta ttacatcctg tacaccacag tggttcggcc accacaaagc
    13441 ggttcatgtc ggctccttga aaatgatatg aaacattgca tttcatattg cataaaggat
    13501 gctattctga ttaactcaat atattcaata agttatgtat taactggatt ctgaacggta
    13561 caaaaatgat ggtcattaac gttcagcaaa aaatggattc gacacccgtg tcgcagggga
    13621 aagcgagaat gtgatcttga tcacgagaag tgaggtatgc tggccgcaag cgcagccagc
    13681 acggaatgga gcaaagtaat caggcgtgtt ctttcagcca gttcagcaca acgtcgtggt
    13741 gattgctggt tttgaaatcg tcaaagacat gttcgatttt gccatcagcg tcaatcagga
    13801 agctgatgcg atgaatgcca tcgtaggttt tgcccatgaa ggacttttca ccccagacgc
    13861 cgaattgttc gcacacctgg tggtcctcat cagacaggag cgtaaagtta agcagctctt
    13921 tttccgcaaa acgggagagt ttttcgggtt tatcggtgct gatacccagc acatcaacgc
    13981 ccgctttttt caactcatcc atgttatcgc gtaagccgca ggcctgtacg gtacagccgg
    14041 gggtcatggc tttcgggtag aaataaacca gaacacgctg tccctggaag tcggtcaaat
    14101 taacttgttc tccgtcttga tccggcaagc taaatttcgg tgcgatatca ccggctttca
    14161 gtggattcat tacttaactc catcctgttc atcatgttgg gaataattga cgacgttaat
    14221 actgccttgt gcattgagtt ctgtacatag ggctttgaac gcttgctcaa tatttgctgc
    14281 gtccgcagat gcggggctgt gggcggttat ctgaatatgc aactgcgcag cccgttcatt
    14341 ttcagcaggt tgcgtgcgcg acaccagctc cgcaatgttc atatgatgcg cgtcgaaaag
    14401 tgctgtgaag cgttcaatta aatgcgggga gtctgccaca tcgacctgaa cccagacaga
    14461 tgctggcatt ggcggacgcg gacgcgccgt cgtgcgcttc atcacgatta aaagatccag
    14521 ctcggcacct ttcaacggta acgttgattc aatcagagta atggcattcc atgaaccgga
    14581 aagcagcatg ataaacgtga actcttctcc cagcatcgcc aggcgactgt cttcaatatt
    14641 acagccgcaa ctactgacat gacgggtgat ggtgttcaca attccagggc gatcggcacc
    14701 caatgcagtg atcaccagat aatgttgcga tgacagtgtc aaactggtta ttcctttaag
    14761 gggtgagttg ttcttaagga aagcataaaa aaaacatgca tacaacaatc agaacggttc
    14821 tgtctgcttg cttttaatgc cataccaaac gtaccattga gacacttgtt tgcacagagg
    14881 atggcccatg ttcacgggaa gtattgtcgc gattgttact ccgatggatg aaaaaggtaa
    14941 tgtctgtcgg gctagcttga aaaaactgat tgattatcat gtcgccagcg gtacttcggc
    15001 gatcgtttct gttggcacca ctggcgagtc cgctacctta aatcatgacg aacatgctga
    15061 tgtggtgatg atgacgctgg agctggctga tgggcgcatt ccggtaattg ccgggaccgg
    15121 cgctaacgct actgcggaag ccattagcct gacgcagcgc ttcaatgaca gtggtatcgt
    15181 cggctgcctg acggtaaccc cttactacaa tcgtccgtcg caagaaggtt tgtatcagca
    15241 tttcaaagcc atcgctgagc atactgacct gccgcaaatt ctgtataatg tgccgtcccg
    15301 tactggctgc gatctgctcc cggaaacggt gggccgtctg gcgaaagtaa aaaatattat
    15361 cggaatcaaa gaggcaacag ggaacttaac gcgtgtaaac cagatcaaag agctggtttc
    15421 agatgatttt gttctgctga gcggcgatga tgcgagcgcg ctggacttca tgcaattagg
    15481 cggtcatggg gttatttccg ttacggctaa cgtcgcagcg cgtgatatgg cccagatgtg
    15541 caaactggca gcagaagggc attttgccga ggcacgcgtt attaatcagc gtctgatgcc
    15601 attacacaac aaactatttg tcgaacccaa tccaatcccg gtgaaatggg catgtaagga
    15661 actgggtctt gtggcgaccg atacgctgcg cctgccaatg acaccaatca ccgacagtgg
    15721 tcgtgagacg gtcagagcgg cgcttaagca tgccggtttg ctgtaaagtt tagggagatt
    15781 tgatggctta ctctgttcaa aagtcgcgcc tggcaaaggt tgcgggtgtt tcgcttgttt
    15841 tattactcgc tgcctgtagt tctgactcac gctataagcg tcaggtcagt ggtgatgaag
    15901 cctacctgga agcggcaccg cttgcggagc ttcatgcccc ggctggaatg attttgccgg
    15961 tgacctccgg tgattatgca atcccggtga ccaacggtag tggtgctgtc ggtaaggcgc
    16021 tggacattcg tccaccagcc cagccgctgg cactggtttc tggcgcgcgt acccagttca
    16081 cgggcgatac cgcttcattg ctggtggaaa atggtcgtgg caatactctg tggccgcagg
    16141 tggttagcgt gctgcaggcg aaaaactaca ccatcaccca acgtgatgat gctggtcaga
    16201 cactgaccac cgattgggta caatggaacc gtctggacga agacgagcag tatcgtggtc
    16261 gttatcaaat ctctgttaag ccgcagggtt atcagcaggc ggttacggtt aaactgttga
    16321 acctggaaca ggcgggcaaa ccggttgcag acgcggcttc catgcagcgt tacagcacgg
    16381 agatgatgaa cgttatttcc gccggtctgg ataaatctgc cactgacgcc gcgaacgctg
    16441 cgcaaaatcg tgcctccacc actatggacg tacaaagtgc agctgatgac accggtttac
    16501 caatgctggt cgtacgcggg ccgttcaatg tggtttggca acgtctgcca gcggcgctgg
    16561 aaaaagtggg catgaaagtg accgacagca cccgttcgca gggcaacatg gccgtaactt
    16621 ataagccgct gtctgacagc gactggcagg aactgggcgc gagcgatcca ggcctggcat
    16681 ccggtgacta taaactgcag gttggcgatt tagataaccg cagcagccta cagttcatcg
    16741 atccgaaagg tcatactctg actcagagtc agaacgacgc gctggtagct gtcttccagg
    16801 ctgcgtttag caagtaaaaa tacagggctg gaatcatccg gccctttttt ctgatatgat
    16861 acgcaaacgt gtgcgtcggc aggaaaacgc gattttagcg gtaattcgca cgaaatttgt
    16921 ttgtcggacg tagttcggat aaggcgttca cgccgcatcc gacaaaacat ccggcacacc
    16981 agacagcaaa agattttaaa acgttaattc acacccagga gtgataaaga tgcaaaagca
    17041 agctgagttg tatcgtggta aagcgaaaac cgtatacagc acggaaaacc cggacctgtt
    17101 ggtgctcgaa ttccgcaatg atacgtcagc aggggatggc gcgcgcattg agcagtttga
    17161 tcgcaaaggt atgttgaaca acaagttcaa ctacttcatt atgagcaaac tggctgaagc
    17221 gggtatcccg actcaaatgg agcgtctgct ctccgatacc gaatgtctgg tgaaaaagct
    17281 ggatatggtg ccggttgagt gtgtcgtgcg taaccgtgct gcgggctctc tggtgaaacg
    17341 tcttggaatc gaagaaggta ttgagctgaa cccgccgctg ttcgatctgt tcctgaaaaa
    17401 cgatgccatg cacgatccga tggtcaacga atcttactgc gaaacctttg gctgggtgag
    17461 caaagagaac ctggcgcgta tgaaagagct gacctacaaa gcgaacgacg tgctgaaaaa
    17521 actgttcgat gatgctggtc tgattctggt cgacttcaag ctggagtttg gtctgtacaa
    17581 aggtgaagtg gtactgggcg atgagttctc cccggacggc agccgcctgt gggacaaaga
    17641 aacactggag aaaatggaca aagaccgttt ccgccagagc ctcggtggcc tgatcgaagc
    17701 ctatgaagcc gtcgcccgcc gcctgggtgt acagctggac tgatttttct gttcatcatc
    17761 ttgccgtgca gctggcacgg caagacaacc gctctcggaa tatatgcatt gaacgctcat
    17821 tcctccgcca tttcatcccg ctttcctgtg gtaatcttcc tctgaaccac ctacgatcat
    17881 ttctataatg aatatattgt tgaggtatct atgcgttggc aagggcgacg tgaaagtgac
    17941 aatgtcgaag acaggcgtaa cagctccggc ggtccgtcaa tgggcggtcc cggttttcgc
    18001 ctgccaagcg gtaaaggcgg gctgatttta ctgatagtcg tgctggttgc aggctactat
    18061 ggtgttgatt taaccgggtt gatgaccgga cagccggttt cccaacagca atcaacacgg
    18121 tcaattagcc caaatgaaga cgaagccgca aaattcacct cggtgattct ggcaaccacg
    18181 gaagacacct ggggacagca attcgagaag atgggtaaaa cctatcagca accgaagctg
    18241 gtcatgtatc gtggaatgac gcgtaccggc tgcggggcgg gccagtccat tatgggaccg
    18301 ttctattgcc cggcggatgg cacggtttat atcgatctct ccttctatga tgacatgaaa
    18361 gacaaactcg gcgcggatgg cgattttgcc caggggtacg ttatcgccca tgaagtcggt
    18421 catcatgtgc agaaattgtt aggcatcgag ccgaaagttc gtcaactgca acaaaacgcg
    18481 acgcaggcgg aagtaaaccg cttatctgtg cgtatggaac tccaggccga ctgttttgcc
    18541 ggtgtctggg ggcataacat gcagcagcaa ggcgatctgg aagaggcgct gaacgcggcg
    18601 caggccatcg gcgatgaccg tttacaacag caaagtcagg ggcgagtagt accagacagt
    18661 ttcactcatg gtacttctca gcaacgctac agctggttta aacgtggttt cgacagcggc
    18721 gatccggcac aatgcaatac ttttggtaaa agcatttaac ttccggggca aggatggctg
    18781 aactgactgc gcttcacaca ttaacagcgc aaatgaaacg tgaagggatc cgccgcttgc
    18841 tggtgttgag cggggaagag ggttggtgtt ttgatcatgc gcttaagttg cgtgatgcct
    18901 tacctggcga ctggctgtgg atttcgccgc agccagatgc tgaaaaccac tgttctccct
    18961 cggcgctaca aactttactt gggcgcgagt tccggcatgc ggtattcgac gcccgccacg
    19021 gctttgatgc cgctgccttt gcggcactta gcggaacgtt gaaagcggga agctggctgg
    19081 ttttgttact ccctgtatgg gaagagtggg aaaaccaacc tgatgccgac tcgctgcgct
    19141 ggagtgattg ccctgaccct attgcgacgc cgcattttgt ccagcatctc aaacgcgtac
    19201 ttacggcgga taacgacgct attctctggc ggcaaaacca gccgttctcg ttggcgcatt
    19261 ttaccccccg tactgactgg caccccgcga ccggcgcacc acaaccagaa caacagcaac
    19321 tcttacagca gctactgacc atgccgccgg gcgtggcagc ggtaacggct gcgcgtgggc
    19381 gcggtaagtc cgcgctggca gggcaactca tttctcgtat tgcgggcagt gcgattgtca
    19441 ccgcgcccgc aaaagcggca acggatgtac tggcacaatt tgcgggcgag aagtttcgct
    19501 ttattgcgcc ggatgccttg ttagccagcg atgagcaagc cgactggctg gtggtcgatg
    19561 aagccgcagc catacctgcg ccgttgttgc atcaactggt atcgcgtttt cctcgaacgt
    19621 tgttaaccac tacggtgcag ggctacgaag gtaccggacg tggttttttg ctgaaatttt
    19681 gcgctcgctt tccgcattta caccgttttg aactgcaaca gccgatccgc tgggcacagg
    19741 gatgcccgct ggaaaaaatg gttagtgagg cactggtttt taacgatgaa aacttcaccc
    19801 atacaccaca aggcaatatc gtcatttccg catttgaaca aacgttatgg cgaagcgatc
    19861 cagaaacgcc gttaaaggtt tatcagttat tgtctggtgc gcactaccgg acctcgccgc
    19921 tggatttacg ccggatgatg gatgcaccag ggcaacattt tttacaggcg gctggcgaaa
    19981 acgagattgc cggggcgttg tggctggtgg atgagggcgg attatctcaa gaactcagtc
    20041 aggcggtatg ggcaggtttt cgtcgcccgc ggggtaatct ggtggcccag tcgctggcgg
    20101 cgcacggcag caatccactg gcggcgacat tgcgcggacg gcgggtcagc cggattgcag
    20161 tccatccggc gcgtcagcgc gaaggcgttg ggcaacagct cattgccagc gctttgcaat
    20221 ataggcctgg cctcgactat ctttcggtga gttttggtta caccggggag ttatggcgtt
    20281 tctggcaacg ctgcggtttt gtgctggtgc gaatgggtaa tcatcgtgaa gccagcagcg
    20341 gttgctatac ggcgatggca ctgttaccga tgagtgatgc gggtaaacag ctggctgaac
    20401 gtgagcatta ccgtttacgt cgcgatgcgc aagctctcgc gcagtggaat ggcgaaacgc
    20461 ttcctgttga tccactaaac aatgccgtcc tttctgacga cgactggctt gaactggccg
    20521 gttttgcttt cgctcatcgt ccgctattaa cgtcgttagg ttgcttattg cgtctgctac
    20581 aaaccagtga actggcatta ccggcgctgc gtgggcgttt acagaaaaac accagtgacg
    20641 cgcagttatg taccacactt aaactttcag gccgcaagat gttactggtt cgccagcggg
    20701 aagaggccgc gcaggcgctg ttcgcactta atgaggttcg cactgagcgt ctgcgcgatc
    20761 gcataacgca atggcaattt tttcactgac tccttcaatt ttctcccatg gtcattgtga
    20821 gtcagcggcg taaaattaat gacatcagtt aaggagttcg ccatgaaaca tgaccatttt
    20881 gttgttcaaa gcccggataa acctgcgcaa cagttgttac tgcttttcca cggtgtcggg
    20941 gataacccgg tggcaatggg ggaaataggc agctggtttg cgccgctatt tcctgatgcg
    21001 ctggtggtga gtgtcggagg cgcggagccg agcggtaatc cggcggggcg tcagtggttt
    21061 tcggtgcagg gtattacgga agataatcgc caggcgcgtg tggatgccat catgccgacg
    21121 tttattgaga tggtgcgcta ctggcagaaa cagagcggag taggggctaa cgccacagcg
    21181 ctaatcggtt tctctcaagg cgcgattatg gcgctggaga gcattaaagc cgaaccgggc
    21241 cttgcttcac gcgtcattgc ttttaacgga cgctatgcga gcctgccgga aactgcgtct
    21301 acagccacga caattcacct gattcatggc ggtgaagatc cggttatcga tctggcgcat
    21361 gcggttgcgg cgcaggaagc gttaatcagt gccggtggtg acgtgacgct ggatatcgtg
    21421 gaggatttag gccatgcgat cgacaatcgc agtatgcaat ttgccctgga tcacttgcgt
    21481 tataccattc cgaaacatta tttcgacgaa gcgctaagtg gcggtaagcc tggcgatgat
    21541 gatgtgattg aaatgatgta aaagacagat tacctggcct gataagacgc gtgagcgtcg
    21601 catcaggcat cgttcaccat tgccggatgc ggcgttaacg ccttatccgg cctacggttg
    21661 agcactcagc ttacttcttc ggttgatcct ttttcggcca gtcgtcgtca tcgtcccact
    21721 tatcgttgaa atcacgatgc gggggaagct ccggcttgtt ggcgagaaat ttcttgtggt
    21781 cgacgcgctt gagatcttta atcacgttca gcaggacgcc taccaaaaag acaatcacca
    21841 gaatccacca atatttagcc agccagtcca tgcttatttc ctcttgcaga accactcatc
    21901 aggcgacgag ctgttccatg atacgttgat acatacgggc aagtagctgc aggtcggcag
    21961 cgttcacaca ttcattaatt ttatgaatag tggcattgac cggcccgagt tccaccacct
    22021 gcgcccccat gcgagcaata aagcgcccgt cggacgttcc gcctgtggtc agtagctgcg
    22081 gtttaatttc attatagtgc tcaaccgcgt taacgaccgc atccaccagt ttaccgcgcg
    22141 cggtcaaaaa tggctgcccg gaaagccacc aatccaccgt atagcgcagt tgatgttttt
    22201 caagcagggc aagcacctgc gctttgatca tctcatcagt cagttcggtg ctgaagcgga
    22261 agttaaactg cacaaacagt tcacccggaa taacgttgtt actgcccgtt cccgcctgaa
    22321 tattggcaat ctgcatactg gtcgccggga agaattcatt gccctgatcc cactcaatag
    22381 ccactaattc attaaggaaa ggtgctgcgc gatgtactgg attgtcagcc agatgtgggt
    22441 aggccacatg cccctgcacg ccatgaatgg taaggttgca ggttaacgat ccgcgacgac
    22501 catttttcac cacatcacca accacttcga tactcgacgg ttcgccgacc agacagtaat
    22561 cgagacgctc attacgcgcc attaacgctt cgacgacttt taccgtaccg ttgtgggcac
    22621 tggcttcttc atcagaggtg atcagaaatg ccagtcgtcc cgtatggttg ggatgttgtg
    22681 cgacaaaacg ttctgctgct accaccatcg ccgccagcga gcctttcata tctgccgcac
    22741 cgcgcccgaa taacatgcca tcacgaatgg tgggttcaaa cggcggattg atccaacgat
    22801 cggcgtcgcc aggcggcacc acgtcggtat gcccggcaaa ggccaatgtt tcaccttgcc
    22861 cacgccatgc ccaaaaattc tgcgtatcgg caaagtccat gcgttcaacg gtaaaaccga
    22921 tcgcctgcaa acgatcaatc aacaaagcct ggcatcctgc gtcatcagga ctcagggaag
    22981 ggcggcgaat aagctgttgt gtcagctcaa taaccgggca cgacatagac tacacctcat
    23041 ggaaaaattg ctgataactg gaatcactga aacccagcag cataggctta ccgggcgcgc
    23101 agagcaatgg acgtttgata attgcaggca tttcagtcat taatgccgcc gctgaggccg
    23161 catcggtgat tttattgcgg gtggtttcgt ccagtttacg ccaggttgta ccacgggtgt
    23221 tgagtaacgc ctcccaacct aattcgttga taaaaccgtt caataattcg ctgtccagcc
    23281 catcgacgcg gtaatcatga aaacgatagt cgatgttatt ggcttccagc caacggcgag
    23341 cctttttaat ggtgtcacaa tttttgatac cgtaaagtgt aaccataatg aatccttttt
    23401 cgatttatga gaataaatac tcatttaagg gtgaaaacat tttgcctgca aatgattatc
    23461 aggaaatata acattatcga agtattcaaa ttaacaacta ttaaataata ttaaacattt
    23521 ttaacgtaaa cattaagaat attcatcttg ttatctgtct gtattttaac ctgaattaaa
    23581 tcagtattag atgccgtatg aatatctcga aatgttgctg tgtatcacat acaaatcatc
    23641 accgcaggac catagtattt acataagatt tgtccccccg gaggtagtca tcgcagcaat
    23701 cgagtaaatt agcttcacca cggtatattg tttccgtaga atacccataa ttataagaga
    23761 ggttgttatg attgaacgtg aactggggaa ctggaaagac tttatcgaag ttatgcttcg
    23821 taagtaattc ctgagacaaa gtataaacag caagaacccg ctgagcaggt tcttaatcgg
    23881 aaagttgaaa aaggcgacac attggcatgt cgcctttttt attgcttatt ccgggcgcgg
    23941 ctttagcggg aagcggcggc gcaccagcac aaagaacagc ggcacgaagt aaatagccag
    24001 aatagtggct gaaatcatcc cgcccattac gccagtaccc accgcatgct gaccaccgga
    24061 accggcaccc gtgctggttg ccattggcaa tacgccgaag ataaatgcca gcgaggtcat
    24121 cagaatcggg cgtaaacgct gacggcaggc gtggagcgtc gcttcaaaca ggtcgtggcc
    24181 tttttggttc atctcgttag caaactcgac gatcaggatg gcgtttttcg ccgataaacc
    24241 aatgaccgtt aacaggccca cctggaagta aacgtcgttt tccagcccgc gcatccaggt
    24301 tgccagcagc gcgccgatta cccccagcgg cacgaccagc attaccgaga acggcaccga
    24361 ccagctttca tacaacgcag ccagacacag gaataccacc agcaaggaaa tggcgtacag
    24421 cgccggagcc tgcgcgccgg aaagtcgctc ctgatacgac atcgccgtcc actccagacc
    24481 aaagccgttt ggcagctgct tcactaacga ttccataata tccatcgccg taccggtact
    24541 gacccccggt gcggcttccc caacaatctc aaccgcagaa taaccgttat agcgttccag
    24601 acgcggcgag cctgtttccc agcgtgaggt cgcgaaagca gagaagggca ccatgccgcc
    24661 atctttattt cggacatacc agagattgat gtcatctggc agcatgcgat acggcgcagc
    24721 tgcctgcaca tagactttct tcacgcgacc gcgatccata aagtcattca catagctcga
    24781 accccaggcg gtttgcagtg tgtcgttaat atcgtcgata gcaacgccca gcgcctgagc
    24841 tttacgctgg tcgatatcaa tctgcaactg cggactgtcg tcgaggccgt tatggcgcac
    24901 acgggttagc tccgggtttt ccgccgccag cgccagcaac tggttacgtg ctgccatcag
    24961 cgcatcgtga cctgctccag cgtggtcctg caactccata tcaaaacccg cagaactacc
    25021 aagaccgcta attgctggcg ggctgctggc gataacgcga gcttctttaa tcttgttaaa
    25081 cgcctttgtt gcacgctcga taatggcaaa cgaggtgccg gtcttactgt cgcgttcgct
    25141 ccagtctttc aggcggataa acattcgcgc cacgttttgc ccgttacccc cagggccaga
    25201 accaacggtg gcaaacaccg acatgatgtt gtctttttca tgggtgaagt aatatttctc
    25261 gatttgctca acgactttca gggtctgttg ttgggttgaa ccgctgggca attgtaccga
    25321 ggtagtaaac atgccacggt cttccagcgg taagaacgac gtcggcaaac gcaggaacag
    25381 gaacaccatg ccgccaagca gcaggacata aatcacaatc cagcgcaggc tgcggtggag
    25441 aattttcgcc accccttttt cgtagcgttc ggcgttgcgg ttaaacatct ggttaaacca
    25501 ggcaaaaaag cctttttgcc cgtgatgctc accttttttc agtggcttaa gtagtgtggc
    25561 acacagagcc ggagtgagga tcatcgctac cagtactgac agcaccatcg ccgcaacaat
    25621 ggtaatagag aactggcgat agatggcacc ggtggtgccg ccgaagaagg ccattggtac
    25681 aaataccgcc gaaagcacca tcgcaatccc gaccagtgcc ccctggatct gccccatcga
    25741 tttacgtgtg gcttcgcgag gagtgagtcc ttcctcactc ataatacgtt cgacgttttc
    25801 caccaccacg attgcgtcat ccaccagcag accgatcgcc agtaccatcg cgaacatggt
    25861 taaggtgttg acgctgtaac cgaaggcgta aagtacggag aaggttccca tcaacaccac
    25921 cggcacggcg atagtgggta tcagcgtggc gcggaagttt tgcaggaaca gatacataac
    25981 gaggaaaacc agggcgatag cttccagcag cgttttcacc acgtcttcaa tggaggcttt
    26041 aacaaacgag gtggtttcat acgccacctt gtattccagt ccgtgcggga agtactgcgc
    26101 cagctcgtcg agacgattga gcaccagctc cgctgtcgcc atttcgtttg cgccggaggc
    26161 cagttttacc cccagcccgg aggctggctt accattgaag cggctaagat aatcgtattt
    26221 ctccgccccc atttcgacgg tggcgacatc gcccagcctt acctctgagc cgtcttgatt
    26281 cacccgcaag gtgatatcgc ggaactgttc tggtgtttgc agcagtgact gggcgttaat
    26341 ggtggcgttg agcgcctgct tatcgacgga aggtgtacca ccaagttgcc caaccgcaat
    26401 ctgcgcgttc tgtgactcaa tggcatcagt gacatcttta gccgtcatct ggaaactgtt
    26461 gagtttcgcc gggtccagcc agatacgcat ggaatattgc gaaccatagg catcgatatc
    26521 cccgacgcca ttcacgcggc ttaacgggtc ctgaatatta ctggcaacat aatcggcaat
    26581 atcctgttta tccatcgaac catcggtaga gacgaaagca atggtcagaa tattggtatc
    26641 gccggtttta cgcaccgtca cgccctgatt ttgcaccgcc tgcggtaact ttcgcatggc
    26701 tgattgcagc tggttttgta cttgctgcac ggcttcgtcc ggatcggtgc ctgctttaaa
    26761 acttaaagtg acagatgcct gaccgatgcc actgctctga gatgacatat acatgagatt
    26821 atcgaggccg gtcatatttt gctcgataac ctgggtcacg gtgttttcca gcgtctgggc
    26881 cgatgcgccg ggatagttag cggtcactcg cacattcggt ggcgcgagat cggggtattg
    26941 ttcaacgggc aatgaaaaaa tcgccagggt ccctgtcaga cacaacagga ttgccagcac
    27001 ccaggcaaaa atggggcgat caataaagaa attcgccatt aaaagaggac ctcgtgtttc
    27061 tgcgtatcgt tgtaggcttg acttgcttca ctgtagcggc aaggtacgag ccaatcgtgg
    27121 agaaaaaaag gagttaatgt aaattatcat gcccttttat actgaacctt aagtgcaagt
    27181 attctttggt cagtaggagg cattacatca actgactttc ctcaccctcc gcagagcgaa
    27241 agctaatact cactaacgtg ccgccgccgg aaggtggtga aaaagtcagc gtcccaccca
    27301 gccgttccgc gcgttcgcgc atgatattca gaccataatg accttcgggt tctttcggtt
    27361 caccgatacc aatcccgtta tcacggatat aaaccgtgtg attgccgtcc ggcgcggtga
    27421 cgcaactgac ggcgatttcg ctggcgttgg cgtgcttcat cgcattcagc accgcttcgc
    27481 gaataatttg caacaaatgc acctgcattt gcgcatccag cgccagggtt ggcagacggc
    27541 agtcgagggt cagtttggcg ctggtttgat tttgtaacgt atccagcatt tccctcaacg
    27601 cggaggggag atccgcctgc tgcagcgtca ggcggaaagt agtcaacagc tcgcgtaact
    27661 gccgataagc atcattcaac gcctgggaaa aatcggccat gatactttgt gcggtggcgt
    27721 tatcttccgg tatcgaacgc ttcagtaacg tcaactggat acgtaagtaa gaaagtacct
    27781 gagccagcga gtcgtgcaat tcgcgggcga tggttgcacg ttcttccatc aacaataact
    27841 gctgaaaatg cttttgcgcc tgattaaagt acaaaccgcg tcccagcatc gacgaaacgc
    27901 tgttaagcag cggttctgat gatgaaacgt gactattttg ccagtgcagt tcgccgtaaa
    27961 ccgtctcttg cattgtcacc ggtaaaatct gcatcggcaa ttccgggttt ggttgccctt
    28021 cgctaatccg ccagttttca ccgacattta actccagata ttcagccgct tcattgtcgc
    28081 gaacaatctg caaaatatgg cggaaacaat gcacatcaat ctggcttgtg ttcagcgcct
    28141 gcgaacattg atacaacacc tccagacgac gcttggcctc gtggagatcg cgggtctttt
    28201 cttctactga cgcctccagc gaaaggtaca atttatgcag ctcgctcgac atctggttaa
    28261 aggtttttgc aagcagacca agctcattcg gcagactggt atccagcggc ggcgagtcga
    28321 actgcccgtg ttcaatacgt tgactggcgg taaccagctg attcagcggg gcaaccacct
    28381 gatggcgtat acggcgcaaa gtaaaaaaga ccagcgtgaa aataccgata ccgccagcca
    28441 gggaaatcgc caccaccagc agcattttgc gttcagcgta gtgctgtaaa gccagcacga
    28501 acaggtctat ctgattaaca taattattaa tattggcctg ataccacggc aaatcgccct
    28561 tgctgagccg attattcatc tccagccagt tggcattcag atgcgcatag cgagttttta
    28621 ctgcttctgg cacataccag acgttgagat tggttaatac cgatgaatgc agtgcctgct
    28681 gaaacaactg gcgatgtgca ttgagttgtg gactgccact ttgcaagtcg tagcccaggc
    28741 ggtaactctg catacgcagc gatccggcaa tattgatagc ctcagcgtcg cgcaaactgc
    28801 tcgccagagt tagcagagcg atacccgtgg aaagaatcga cagcagcaca atgtaaaaaa
    28861 aggcccgggc cagactggcc gagacgggtc gtttaacaat cacacgcgtc ttctccacaa
    28921 aaaatcatta atgttccagt tcgctaaaac gcaatttagt cagtgatggg attatttgat
    28981 ctgccacagt ttatagcgaa atagttactt attctgggca catgaacatt gccgattccc
    29041 ctgtaagtcg ggtaataaca acactcatat aaagaataag gtttttacaa ccaaaaaaga
    29101 aggtcgttat gaatcgtttt attatggcca acagtcagca atgtctgggt tgtcatgctt
    29161 gtgaaatcgc ctgtgtcatg gctcacaatg atgagcaaca tgtcctgagc caacaccatt
    29221 ttcatccccg aattacggtt atcaaacatc agcagcaacg tagtgcagtg acctgtcacc
    29281 attgtgaaga tgcgccctgc gcccgtagct gccctaatgg cgcaatcagc cacgttgatg
    29341 acagcattca ggtcaatcag caaaagtgta ttggctgtaa atcctgcgtg gtggcctgtc
    29401 cgtttggtac gatgcaaatc gtcctgacac ccgtcgcggc aggaaaagta aaagccacgg
    29461 cgcataaatg cgacctttgt gcagggcgcg aaaacggtcc tgcctgtgtc gagaattgcc
    29521 cggcggacgc gctgcaactg gtcactgacg tcgcactctc cggcatggcg aaatcccgcc
    29581 gcttgcgcac cgcacgtcag gagcatcaac cgtggcatgc cagtaccgct gcacaagaaa
    29641 tgccggtaat gagtaaagtc gaacaaatgc aggcaacgcc cgcgcgtggc gagccggata
    29701 aactggcgat tgaagcgcgc aaaaccggtt ttgatgaaat ttatctgcca ttccgtgccg
    29761 atcaggcaca acgggaagcc tcgcgctgcc ttaaatgcgg cgagcacagc gtttgtgaat
    29821 ggacctgccc gctgcataac catattccgc agtggattga actggtgaaa gccggaaaca
    29881 tcgacgccgc cgtcgagctt tctcacctga ccaacaccct gccggaaatt accggacgcg
    29941 tttgtccgca ggaccgtttg tgtgaaggtg cctgtactat tcgcgatgag cacggcgcgg
    30001 taactatcgg caacattgaa cgctacattt cagatcaggc gttggcgaaa ggttggcgtc
    30061 ctgacttaag ccatgtcacc aaagtggaca agcgggtggc gattatcggt gcgggtccgg
    30121 cagggctggc ctgtgcggat gttctgaccc gcaatggcgt ggcggtgacg gtgtacgatc
    30181 gccatccaga aattggtggc ttgctcactt tcggcattcc ttctttcaaa ctggataaat
    30241 ccctgctggc acgccgtcgg gaaatcttca gcgcgatggg gattcacttc gaactcaatt
    30301 gtgaagtggg taaagatgtc tctttggatt cgcttttgga acaatacgac gcggtcttcg
    30361 ttggcgtagg cacttaccgt tccatgaaag cgggtttacc caatgaagat gcgccgggcg
    30421 tttatgacgc gctgccgttc ctcatcgcca acactaaaca ggtgatggga ctcgaagagc
    30481 taccggaaga gccgtttatc aataccgccg gactcaacgt cgtggtactg ggcggcggcg
    30541 acaccgcgat ggactgtgtg cgtaccgcgc tgcgccacgg cgcgagtaac gtcacctgcg
    30601 cttatcgtcg tgatgaagct aacatgccag gctcgaagaa agaggtgaag aacgcccgcg
    30661 aagagggggc caacttcgaa tttaacgtcc agccggtggc gcttgagctg aatgagcaag
    30721 gtcacgtttg cggaattcgt ttcctgcgca cgcgtcttgg cgagccggat gcccaggggc
    30781 gccggcgact tgtaccgatt gagggcagtg aatttgtcat gccagccgat gcggtgatta
    30841 tggtgtttgg cttcaatcca cacgggatgc cgtggctgga gtcgcacggt gtaacggtag
    30901 acaaatgggg ccgcatcatc gcggatgtgg aaagccagta ccgttaccag accaccaatc
    30961 cgaaaatctt cgccggtggc gacgccgtgc gtggtgcgga tctggtggtt accgcaatgg
    31021 cagaaggacg tcatgcggca caggggatta ttgactggct gggggtaaaa tcagtcaaat
    31081 ctcactgata gcctgcgcag acaaacccga cttcacagcg taatataatt gttcatttcg
    31141 cgctgtggag tcggtatgac gcaacaaatc accctcatta aagacaaaat tctctccgat
    31201 aactatttca ccctgcacaa cattacttac gatctcacgc gcaaagatgg cgaagttatc
    31261 cgccataaac gtgaagttta cgatcgcggt aatggtgcga cgatcctcct gtacaacgcg
    31321 aagaaaaaga ccgtggttct gattcgtcag ttccgtgtcg ctacctgggt taatggcaat
    31381 gaaagcgggc agctgattga aacctgcgcc gggctgctgg ataacgacga accggaagtg
    31441 tgcattcgta aagaagcgat tgaagagacg ggctatgaag tgggcgaagt gcgcaaatta
    31501 tttgaactgt atatgtcgcc tggcggtgtg actgagctaa tccacttttt tatcgccgaa
    31561 tacagtgaca atcagcgcgc taacgccggt ggcggtgttg aagatgaaga tattgaagtg
    31621 cttgagctgc cgttcagcca ggcgctggag atgatcaaaa ccggcgagat acgtgacggt
    31681 aagacggtgt tattgcttaa ctatttgcaa acgtcacatt taatggactg aaaaataaca
    31741 ataatatttc gttgtttatt attggctcaa tccgacaaat ctgattgagc cgcgctacct
    31801 gcgcaacgaa gatacgactt gtgctgtttg tttgaacttc tggggtcgta ccgtccatgc
    31861 gctatcgcat tttccttctc ttttttttcg ctttgttgcc gacgtctttg gtgtgggcgg
    31921 cactagcgca acgggcgttt tccgactggc aggtcacctg taataaccaa aacttctgcg
    31981 tggcgcgcaa tacgggcgat cataatggac tggtgatgac cctgagccgc agcgccgggg
    32041 cgcataccga tgccgtttta cgtattgagc gcggcgggtt gaagtcgccg gatgcgtctg
    32101 aaggagagat agcgccacgg ctgctgttag atggtgagcc gttggcgtta agtggtgaca
    32161 agtggcggat ttcaccatgg ttattagtaa ccgatgatac ggcaaccatc accgcgtttt
    32221 tgcagatgat tcaggaaggg aaggcgatca ccttacgcga cggcaatcag accatttctc
    32281 tgagtggctt aaaagcggcg ttgttgttta ttgatgctca gcaaaagcgc gttggcagtg
    32341 aaaccgcgtg gatcaagaaa ggggacgaac cgccgctcag cgtaccgccc gcgcctgcgc
    32401 tgaaagaggt cgcggtggtt aacccaacgc cgacgccact ctcactcgaa gaacgcaacg
    32461 atttgctgga ttatggaaac tggcggatga atggtctgcg ctgctcgctt gatccgttac
    32521 gtcgtgaggt gaatgtcact gcgctgactg atgataaagc gctgatgatg atcagctgtg
    32581 aggcaggggc ctataacacc attgatttgg catggattgt gtcgcgtaaa aagccgctag
    32641 cctcgcgccc ggttcggttg cgtttgccgt tcaacaacgg gcaggagacg aatgaactgg
    32701 aactgatgaa cgcaacattt gatgagaaat cgcgtgaact ggtgacctta gcgaaagggc
    32761 gcggattaag cgattgtggc attcaggcgc gctggcgctt tgacggtcag cgttttcgcc
    32821 tggtgcgtta cgccgcagaa cccacctgcg ataactggca tgggccagac gcctggccca
    32881 cgttgtggat cacccgttaa acaaaatggc ggggacatgt aggccggata agacgctgta
    32941 ggccggataa gacgcgtcag cgtcgcatcc ggcaatcagc atccggcgat cactatcagg
    33001 cacctttcac tcccagcacc ttatgcgctt ttgccacaat attctcggcg gtaaagccaa
    33061 agaacgggaa cagcttatcc gccggagcag attccccata acccgtcatc ccgacaattg
    33121 cccctttcag accaacatac ttgtaccagt aatcggcaat acctgcttcc accgccacgc
    33181 gagccgcaac gttagaaggc aacaccgact cccgatattc ctcatcctgg gcgtcgaaaa
    33241 tatcggtcga gggcagggaa accacgcgaa cattgcgacc ttctcctgct aatttctccg
    33301 cagcttgcag ggtgatttcc atctctgaac cggtggcaat cagaataata tctggcttac
    33361 cgccgctgtc tttcagcaca taaccgccac gagtaatctc tttaacctga tccggcgtac
    33421 gttccacctg ggccagattc tgccttgaga ggatcagtgc cgtcggtccg ttgtggcgct
    33481 caaccgccag cttccagccc accgccgctt ccacctgatc gcacggtcgc caggtgctga
    33541 aatttggcgt taagcgcagg ctggccagtt gctcaacagc ctggtgcgtc ggaccatctt
    33601 cgcccaggcc aattgagtcg tgggtataaa ccataatctg ccgcgctttc atcagtgccg
    33661 ccatccgcgc ggcgttacgg gcgtattcaa caaacatcag gaacgtcgcg gtatacggca
    33721 caaagccgcc gtggtgcgcg atgccgttgg cgatagcggt catgccaaat tcacgcaccc
    33781 cgtagtgaat gtagttgccc gccggatctt ccttcagcga aacagaacct ttccagatgg
    33841 tcaggttgct gggagccaga tccgccgaac cgccgagcag ctccggtagc aaggggccat
    33901 aagcgttaag cgtattttgc gaagccttac gggtggcgat tttcgccgga ttcgcctgca
    33961 actcattgat atacttctga gtcgttttct cccagtcctt cggtaaacca ccgctcatcc
    34021 gacgggtaaa ctcttctgcc agttgcggat gagccttttt ataggcggca aacttctcat
    34081 tccacctctg ctgcgctttt tcgccttttt cgcgggcatc ccaggcgtga taaatatctt
    34141 tagggatctc aaatggcgga tggtgccagc ccagtttttg ccgtgccagc gccacttctt
    34201 cttcccccag cggtgcgccg tgcgcctctt ccttacctgc tttattcggc gatccaaagc
    34261 caatcaccgt acgacagata atcagcgacg gcttatcttt cacgctttgc gcttcaagga
    34321 tcgcttcctt caccgcctgc ggatcgtgac cgtcgatttc atggatcaca tgccagtgat
    34381 aggcttcaaa acgttttgcc gtatcgtcgg taaaccagcc ttctgtttca ccgtcgatgg
    34441 aaataccgtt gtgatcgtaa aaaccaatca gcttgcccag tcccagcgtg cctgccagcg
    34501 aacagacttc gtgggaaata ccttccatca ggcagccgtc gcccataaac acataggtga
    34561 agtgatcgac gatctcatgg tctggctggt taaactgcgc cgccagtgta cgctctgcta
    34621 tcgccagccc gacggcgttc gccaaacctt gtccaagtgg accggtggtg gtttcaacgc
    34681 ctggcgtata accaatctcc gggtggcctg gggttttcga atgcagttga cgaaagttct
    34741 tcagctcttc cagcggcagg tcgtaaccgg tcagatgcag caaactgtag agcagcatcg
    34801 acgcgtgacc gttggaaaga ataaagcggt cgcgatcata ccaggttggg tcggtagggt
    34861 tatgtttaag aaaatcgttc cacagcactt cggcaatatc agccatgccc atcggcgcgc
    34921 cgggatgacc agaattggct ttttgtaccg catccatact gagtgcgcga atcgcattgg
    34981 caaggtcttt tcgggacata taacactccg tggctggttt atagtttggc ggcaagaaga
    35041 tcttccagtt tgcgttgatc aacggcgaac agacgaatgc cttcagacag tttttctacc
    35101 gccatcgcat actgattgtg ctcccaacgg aactccgctt cgctcatggg agctgggcgt
    35161 gggaacgtct gagaaggtgg gattaattta cgtaccactg gcgaaacttt ttcctgcagc
    35221 tccttcagca aattcggtgc gatagtcagt cgatcgcagc cggttaaggc gaggatttgt
    35281 tcggtgcgac ggaagctcgc gcccatcaca atggtttcat agtggtgttg cttatagtag
    35341 tcgtagatat tgcgcaccga tttaacgccc ggatcttctt ccaccacata cgggtccatc
    35401 ggcttgcgtg cctgatacca gtcataaata cgcccgacaa acggcgaaat cagaaaaacg
    35461 cctgcttccg cacaggcccg tgcctgtgca aaagaaaaca gcagcgtcag gttgcagtta
    35521 ataccttctt tttccagctc ttctgccgcg cgaattcctt cccaggtcga agccagcttg
    35581 atcagaatgc gtgatttctc aacgccttgt tgctgataca agtccaccag atggcgcgcc
    35641 ttctcaatac tcttttcttt atcaaaagag aggcgtgcat caacttctgt tgacacgcga
    35701 ccgggtacga ttttgaggat ttcagcaccg aaattgaccg ccagtttgtc acacgctgcg
    35761 accacctgtt gttcctgggt cttgccattt tttttacccc aggcgatagc atcgtctatt
    35821 aaatgctcat attgtgataa tccggcagcc ttgagtaaca gcgaaggatt ggtggtggca
    35881 tcctggggat gataatggcg aatggactca atatcgccgc tgtctgccac gacagtggtg
    35941 aactgtttga tgccgtctaa ctcgttcata ggaaatactc cttgaaaagt aaagtgttag
    36001 atgagtgcgt taattcacac ttctgagaaa tttcgctaaa cgcatcaaaa aagcatagca
    36061 gacaggcatg gtattgctgg attaagcagg taacatcagt gttataggat tattaccaaa
    36121 acattatatt aatggtgaga gtttggactt gctcaaagtc tgtagactcc ggcaaggtaa
    36181 taatgtgcgc cacgttgtgg gcagggggtg ttgcccacac actttatttg tgaacgttac
    36241 gtgaaaggaa caaccaaatg gatgaccagt taaaacaaag tgcacttgat ttccatgaat
    36301 ttccagttcc agggaaaatc caggtttctc caaccaagcc tctggcaacg cagcgcgatc
    36361 tggcgctggc ctactcaccc ggcgttgccg caccttgtct tgaaatcgaa aaagacccgt
    36421 taaaagccta caaatatacc gcccgcggta acctggtggc ggtgatctct aacggtacgg
    36481 cggtgctggg gttaggcaac attggcgcgc tggctggcaa accggtgatg gaaggcaagg
    36541 gcgttctgtt taagaaattc gccgggattg atgtatttga cattgaagtt gatgaactcg
    36601 acccggacaa atttattgaa gttgtcgctg cgctcgaacc gaccttcggc ggaatcaacc
    36661 tcgaagacat taaagcgcca gaatgtttct atattgaaca gaaactgcgc gagcggatga
    36721 atattccggt attccacgac gatcagcacg gcacggcaat catcagcact gccgccatcc
    36781 tcaacggctt gcgcgtggtg gagaaaaaca tctccgacgt gcggatggtg gtttccggcg
    36841 cgggtgccgc agcaatcgcc tgtatgaatc tgctggtagc gctgggtctg caaaaacata
    36901 acatcgtggt ttgcgattca aaaggcgtta tctatcaagg ccgtgagcca aacatggcgg
    36961 aaaccaaagc cgcatatgcg gtggtggatg acggcaaacg taccctcgat gatgtgattg
    37021 aaggtgcgga tattttcctg ggctgttccg gcccgaaagt gctgacccag gaaatggtga
    37081 agaaaatggc tcgggcacca atgattctgg cgctggcgaa tccggaaccg gaaattctgc
    37141 cgccgctggc gaaagaagtg cgtccggatg ccatcatctg caccggtcgt tctgactatc
    37201 caaaccaggt caacaacgtc ctttgcttcc cgttcatctt ccgtggcgcg ctggacgttg
    37261 gcgcaaccgc catcaacgaa gagatgaaac tggcggcggt acgcgcgatt gcagaactgg
    37321 cccatgcgga acagagcgaa gtggtggctt cagcgtatgg cgatcaggat ctgagctttg
    37381 gtccggaata catcattcca aaaccgtttg atccgcgctt gatcgttaag atcgctcctg
    37441 cggtcgcgaa agcggcgatg gagtcgggcg tggcgacgcg tccgattgct gatttcgacg
    37501 tctacatcga caagctgact gagttcgttt acaaaaccaa cctgtttatg aagccgattt
    37561 tctcccaggc tcgcaaagcg ccgaagcgcg tagttctgcc ggaaggggaa gaggcgcgcg
    37621 ttctgcatgc cactcaggaa ctggtaacgc tgggactggc gaaaccgatc cttatcggtc
    37681 gtccgaacgt gatcgaaatg cgcattcaga aactgggctt gcagatcaaa gcgggcgttg
    37741 attttgagat cgtcaataac gaatccgatc cgcgctttaa agagtactgg accgaatact
    37801 tccagatcat gaagcgtcgc ggcgtcactc aggaacaggc gcagcgggcg ctgatcagta
    37861 acccgacagt gatcggcgcg atcatggttc agcgtggcga agccgatgca atgatttgcg
    37921 gtacggtggg tgattatcat gaacatttta gcgtggtgaa aaatgtcttt ggttatcgcg
    37981 atggcgttca caccgcaggt gcaatgaacg cgctgctgct gccgagtggt aacaccttta
    38041 ttgccgatac ctacgtcaat gacgaaccag acgcagaaga gctggcggag atcaccttga
    38101 tggcggcaga aactgtccgt cgtttcggta ttgaaccgcg cgttgctctg ttgtcgcact
    38161 ccaactttgg ttcttctgac tgcccgtcgt cgagcaaaat gcgtcaggcg ctggaactgg
    38221 tcagggaacg tgcgccagaa ctgatgattg atggtgaaat gcacggcgat gcggcgctgg
    38281 tggaagcgat tcgcaacgac cgtatgccgg acagcccttt gaaaggttct gccaatattc
    38341 tggtgatgcc gaacatggaa gctgcccgca ttagttacaa cttactgcgc gtttccagct
    38401 cggaaggtgt gactgtcggt ccggtgctga tgggcgtggc gaaaccggtt cacgtgttaa
    38461 cgccgatcgc atcggtgcgt cgtatcgtca acatggtggc gctggccgtg gttgaagcgc
    38521 aaacccaacc gctgtaattc ttttttaact ctcacgctta ccctgaatat tcagggtaag
    38581 cggtttggct gcaatatatt agtaaagctt attactgagt ttgcgaataa taaaaaaagc
    38641 agtctatata atatctcgat attattttat ttatattcat gcgttgcata tgaaagttta
    38701 tgcaccacag cgaatatctc tccattctta gtgatctacc tcacctttta aacgcgcttg
    38761 ccgaattttg ttatttactc tgacgaaaaa ttgtcacgat acacgaaagt ttttcacagg
    38821 cggcgactca tggataaaga acgcatcatt caggaatttg tgcccggcaa acaggtcaca
    38881 ctggcgcatc tcatcgcgca tcccggtgag gaactggcga agaaaatcgg cgttccggat
    38941 gcgggcgtaa ttggcattat gacgctaact cccggcgaaa cggcgatgat cgtcggtgat
    39001 ttagcgctga aagccgctga tgtgcatatc ggttttctcg accgcttcag cggtgcattg
    39061 gtgatttatg gctcggtggg cgcagtagaa gaagcgttat cacaaaccgt cagcggttta
    39121 ggtcgcttat taaattatac gctttgtgaa atgaccaaaa gttaatacga ggcattcatg
    39181 aaacgaattg cgtttgtggg tagcgtcggg gcaggaaaaa caacgttatt taatgcgttg
    39241 caggggaatt ataccctcgc cagaaaaaca caggccgtgg aatttaatga taagggcgat
    39301 attgatacgc cgggtgaata ttttagccat ccccgctggt atcacgcctt aattaccacg
    39361 ctacaggatg tagacatgct gatctatgtc catggcgcaa atgacccgga aagtcggttg
    39421 cctgccgggt tgctggatat tggcgtcagc aaacggcaaa tcgccgtcat cagtaaaacg
    39481 gacatgccag atgctgacgt cgccgcaacg cgaaagttat tgcttgagac gggtttcgaa
    39541 gagccgattt tcgagctaaa cagccacgac ccgcaaagcg tgcagcaact ggtggattat
    39601 ctggcatctc tcaccaaaca ggaggaagca ggtgaaaaaa ctcatcacag cgaatgatat
    39661 tcgtgaagca cacgcgcgcg gcgaacaggc aatgtccgtg gttctgcgcg ccagcattat
    39721 taccccggaa gcccgtaaag tggcggatct actgggtttt accatcaccg aatgtgatga
    39781 atcgatcccg gtaacggcgt ccgtgcccgc cagcgtgcca gccgacaaaa ccgaaagcca
    39841 gcgcatccgc gaaaccatca ttgcccagct gccggaaggc cagtttaccg aaagcctggt
    39901 cgcacagctg atggaaaaag tgatgaagga aaaacagtcg ctggagcagg gcgcaatgca
    39961 gccgagcttc aaatcggtga ccggcaaagg cggcatcaaa gtgattgatg gcagcagcgt
    40021 caagtttggt cgttttgatg gcgcacaacc gcattgcgtg ggcttgactg atcttgtgac
    40081 cggagacgac ggcagcagca tggccgccgg ttttatgcag tgggagaacg cattcttccc
    40141 gtggaccctg aactacgacg aaatcgacat ggtgctggaa ggggagctcc atgttcgcca
    40201 cgaaggcgaa accatgatcg ccaaagcagg cgacgtgatg tttatcccga aaggctccag
    40261 catcgaattt ggtacgacat ccagcgtgaa attcctgtat gtcgcctggc cggctaactg
    40321 gcaatcccta tgaaagattt catcaccgaa gcatggctaa gagcgaacca tacgctcagc
    40381 gaaggcgcag agatccatct ccctgcggac agtcgcctga cgccctctgc ccgggagttg
    40441 ctggaaagcc gccatctgcg catcaagttt attgacgagc agggccgcct gtttgttgac
    40501 gatgaacagc agcagccgca gcccgttcat gggctgacca gtagcgatga acatccgcag
    40561 gcgtgctgcg aactgtgccg ccagccggtg gcaaaaaagc cggatacgct gactcacctg
    40621 tcggcggaaa aaatggtcgc caaaagcgat ccgcgtctgg gttttcgcgc ggttctcgac
    40681 agcaccattg cgctggcagt gtggctgcaa attgaactgg cggaaccgtg gcagccgtgg
    40741 ctggcggata tccgttcgcg tcttggcaac attatgcgcg ccgatgcgct gggggaaccg
    40801 ctgggctgcc aggcgattgt cgggctttct gacgaagatc tgcatcgact ttctcaccag
    40861 ccgctgcgct atctcgacca cgatcatctg gtgccggaag ccagccacgg gcgcgatgcg
    40921 gcgttgctca atctgctacg taccaaagtg cgggaaaccg aaaccgtagc ggcgcaggtg
    40981 tttatcaccc gcagctttga agtattacgc ccggacatct tgcaggcgct gaaccgcctt
    41041 tcgagcacgg tctacgtaat gatgatcctg agcgtaacga agccgccgct aacggtgaag
    41101 cagatccaac aacgactggg ggaaacgcaa tgattattga acgttgtcgt gaactggcgt
    41161 tgcgagcgcc cgccagagtg gtttttccgg atgcgttaga tcaacgtgtg ctgaaagctg
    41221 cgcaatattt acatcaacaa ggtctggcaa cgcccattct ggtcgctaat ccgtttgaac
    41281 ttcgtcagtt tgcgctcagt cacggcgtgg cgatggacgg gctacaggtg attgatccgc
    41341 atggcaacct cgcaatgcgg gaagaatttg ctcatcgctg gctggcccgc gcgggcgaaa
    41401 aaacgccgcc ggatgcgctg gaaaaactta ccgatccgct gatgttcgcc gcagcaatgg
    41461 tcagcgccgg taaagcggat gtctgtatcg cgggcaacct ctcttccacg gcgaatgtgc
    41521 tgcgtgccgg attacgtatt atcggcttgc agccaggctg taaaacgctc tcatccattt
    41581 tcctgatgct gccacagtac agcggtccgg cgttgggctt tgccgattgc agcgtggtgc
    41641 cacagccgac ggcggcgcaa ctggcggata tcgcgcttgc cagtgccgaa acctggcgcg
    41701 ccctcaccgg agaagagccg cgcgtggcga tgctgtcgtt ttccagtaac ggtagcgccc
    41761 gtcacccctg cgttgccaac gtccagcagg cgacagaaat cgtccgtgag cgcgcaccaa
    41821 agctggtggt tgatggcgag ttgcagtttg acgccgcctt cgtgccggaa gtggcggcgc
    41881 aaaaagcgcc tgccagcccg ctacagggca aagccaatgt gatggttttt ccgtcgctgg
    41941 aagccggaaa tattggctac aaaatcgcac aacgactcgg cggatatcgt gccgtcgggc
    42001 cattgataca aggacttgcc gcgccgatgc acgatctctc tcgtggttgt agcgtgcagg
    42061 aaattatcga gctggcgctg gtggcagctg tgccgcgtca gacagaagtg aaccgcgaaa
    42121 gcagtttaca aacactggtt gaatgaacgg tcccgttctg gaccctgata aagaggaaag
    42181 cacaatggaa gcattaggaa tgatcgaaac ccggggcctg gttgcactga ttgaggcttc
    42241 tgacgcgatg gttaaagctg cgcgcgtcaa gctggttggt gttaagcaga ttggcggcgg
    42301 cctgtgtact gccatggttc gtggtgatgt tgcagcctgt aaagccgcga ccgatgcagg
    42361 tgccgcagcg gcacagcgta tcggcgagct ggtatctgta cacgttatcc cgcgtccgca
    42421 cggcgacctg gaagaagtct tcccgatcgg cctgaaaggc gatagcagca acctgtaatc
    42481 accctgtgtg acgtcgattc gggcgtcacg ctctctcctt tttcatttta ccttctgcgg
    42541 agggtagggg gagcttttac ctgaaatacg ccacggaggc gggtatgaaa ctggcagtcg
    42601 tcactggaca aattgtttgt accgtacgcc atcacggact ggcgcatgac aaattgctga
    42661 tggtggaaat gattgatcca caaggtaatc ctgacgggca atgcgccgtc gccatcgaca
    42721 atattggcgc gggaaccggg gagtgggtgt tactggtgag tggcagttcc gcccgccagg
    42781 cgcataaaag cgaaacgtca ccggtcgatc tgtgcgtgat tggcattgtc gatgaggtgg
    42841 tgtctggcgg tcaggtaatt ttccacaaat aaggcagaac atcatgaatc aacaggatat
    42901 tgaacaggtg gtgaaagcgg tactgctgaa aatgcaaagc agtgacacgc cgcccgccgc
    42961 cgttcatgag atgggcgttt tcgcgtccct ggatgacgcc gttgcggcag ccaaagtcgc
    43021 ccagcaaggg ttaaaaagcg tggcaatgcg ccagttagcc attgctgcca ttcgtgaggc
    43081 aggcgaaaaa cacgccagag atttagcgga acttgccgtc agtgaaaccg gcatgggccg
    43141 cgttgaagat aaatttgcca aaaacgtcgc tcaggcgcgc ggcacgccag gcgttgagtg
    43201 tctctctccg caggtgctga ccggcgacaa cggcctgact ttaattgaaa acgcgccctg
    43261 gggcgtggtg gcttcggtaa ctccgtccac taacccggcg gcaaccgtaa ttaataacgc
    43321 catcagcctg attgccgcgg gcaacagcgt catttttgcc ccgcatccgg cggcgaaaaa
    43381 agtctcccag cgggcgatca cgctgctcaa ccaggcgatt gttgccgcag gcgggccgga
    43441 aaacttactg gttactgtgg caaatccgga tatcgaaacc gcgcagcgtt tgttcaagtt
    43501 tccgggcatc ggccttctgg tggtgaccgg cggcgaagcg gtagtagaag cggcgcgtaa
    43561 acacaccaat aaacgtctga ttgccgcagg cgctggcaac ccgccggtag tggtggatga
    43621 aaccgccgac ctcgcccgtg ccgctcagtc catcgtcaaa ggcgcttctt tcgataacaa
    43681 catcatttgt gccgacgaaa aggtactgat tgttgttgat agcgtagccg atgaactgat
    43741 gcgtctgatg gaaggccagc acgcggtgaa actgaccgca gaacaggcgc agcagctgca
    43801 accggtgttg ctgaaaaata tcgacgagcg cggaaaaggc accgtcagcc gtgactgggt
    43861 tggtcgcgat gcggcgaaaa tcgcggcggc aatcggcctg aacgtgccgc aagaaacgcg
    43921 tttgctgttt gtggaaacca ctgcagaaca tccgtttgcc gtgaccgaac tgatgatgcc
    43981 ggtgctgccc gtcgtgcgcg tcgccaacgt ggcggatgcc attgcgctgg cggtgaaact
    44041 ggaaggcggt tgccaccaca cggcggcaat gcactcgcgc aacatcgaaa acatgaacca
    44101 gatggcgaat gctattgata ccagcatttt cgttaagaac ggaccgcgca ttgccgggct
    44161 ggggctgggc ggggaaggct ggaccaccat gaccatcacc acgccaaccg gtgaaggggt
    44221 aactagcgtg cgtacgtttg tccgtctgcg tcgctgtgta ttagtcgatg cgtttcgcat
    44281 tgtttaagga gtaacagatg gcgcacgacg aacaatggct caccccacgt ctgcaaacgg
    44341 cggcaacgct gtgtaaccag acgcccgccg cgacggaatc tccgctgtgg ctgggggtgg
    44401 atctgggcac ctgcgatgtg gtgtcgatgg ttgtcgaccg cgacggtcag tcggtggcgg
    44461 tatgcctcga ctgggccgat gtggtccgcg acggcatcgt ctgggatttc ttcggcgctg
    44521 tcaccattgt tcgtcgccat ctcgacacgc tcgagcagca attcggtcgc cgttttagcc
    44581 atgcggcgac ttcatttcct ccaggtacgg acccgcgtat ttccatcaac gtgctggagt
    44641 ctgctggact ggaagtgagc catgtgctgg atgagccaac cgcagttgcg gatctgctgc
    44701 aactggacaa cgccggtgta gtggatatcg gcggcggcac taccggcatt gccatcgtga
    44761 aaaagggcaa ggtgacgtac tcggcggatg aagcaaccgg cggacatcac atctctctga
    44821 ctctcgccgg aaatcgccgt atttcgctgg aagaggcgga gcagtacaag cgcggtcacg
    44881 gtgaagagat ttggcctacg gtgaaaccgg tgtacgaaaa aatggcggac attgtcgccc
    44941 ggcatattga agggcagggc attactgatt tatggctggc gggcggctct tgtatgcaac
    45001 cgggcgtggc ggaactgttt cgcaaacaat tcccggcgtt acaggtgcat ttaccgcagc
    45061 acagtttgtt tatgaccccg ctggcgatcg ccagtagtgg gcgagagaaa gcggaggggc
    45121 tatatgcaaa atgaattgca gaccgcgctc tttcaggcgt tcgataccct gaatctgcaa
    45181 cgggtaaaaa catttagcgt tccaccggtg acgctttgcg gtccgggctc ggtgagcagt
    45241 tgcggacagc aagcgcaaac gcgtgggctg aaacatctgt tcgtgatggc agacagcttt
    45301 ttgcatcagg cagggatgac cgccgggctg acgcgcagcc tggctgttaa aggcatcgcc
    45361 atgacgctct ggccatgtcc ggtgggcgaa ccgtgcatca ccgacgtgtg tgcagccgtg
    45421 gcgcagttgc gtgagtcagg ctgtgatggg gtgatcgcat ttggcggcgg ctcggtgctg
    45481 gatgcggcga aagccgtggc gttgctggtg acgaacccgg atagcacgct ggcagagatg
    45541 tcagaaacca gcgttctgca accgcgcttg ccgctgattg ccattccaac gaccgccgga
    45601 accggctctg aaaccaccaa tgtaacggtg attatcgacg cggtgagcgg gcgcaagcag
    45661 gtgttagccc atgcctcgct gatgccggat gtggcgattc tcgacgccgc gttgaccgaa
    45721 ggtgtgccgt cgcatgtcac ggcgatgacc ggcattgatg cgttaaccca tgccattgaa
    45781 gcatacagcg ccctgaacgc tacaccgttt accgacagtc tggcgattgg tgccattgcg
    45841 atgattggca aatcgctgcc gaaagcggtg ggctacggtc acgaccttgc cgcgcgcgag
    45901 agcatgttgc tggcttcatg tatggcggga atggcgtttt ccagtgcggg tcttgggttg
    45961 tgccacgcga tggcgcatca gccgggcgcg gcgctgcata ttccacacgg tctggcgaac
    46021 gccatgttgc tgccaacggt gatggaattt aaccggatgg tttgtcgtga acgctttagt
    46081 cagattggtc gggcactgcg aactaaaaaa tccgacgatc gtgacgctat taacgcggta
    46141 agtgagctga ttgcggaagt tgggattggt aaacgactgg gcgatgttgg tgcgacatct
    46201 gcgcattacg gcgcatgggc gcaggccgcg ctggaagata tttgtctgcg cagtaacccg
    46261 cgtaccgcca gcctggaaca gattgtcgga ctgtacgcgg cggcgcaata aatgccggat
    46321 gcgacgcttg ccgcgtctta tcc
//
LOCUS       DAJVQG010000011        45382 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJVQG010000011 DAJVQG010000000
VERSION     DAJVQG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45382
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.011"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(103..762)
                     /gene="pgmB"
                     /locus_tag="OQM83_000598"
     CDS             complement(103..762)
                     /gene="pgmB"
                     /locus_tag="OQM83_000598"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCS1518950.1"
                     /translation="MKLQEVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(759..3026)
                     /gene="ycjT"
                     /locus_tag="OQM83_000599"
     CDS             complement(759..3026)
                     /gene="ycjT"
                     /locus_tag="OQM83_000599"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCS1518951.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEVHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3023..4078)
                     /locus_tag="OQM83_000600"
     CDS             complement(3023..4078)
                     /locus_tag="OQM83_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1518952.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4088..4876)
                     /locus_tag="OQM83_000601"
     CDS             complement(4088..4876)
                     /locus_tag="OQM83_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCS1518953.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(4894..5946)
                     /locus_tag="OQM83_000602"
     CDS             complement(4894..5946)
                     /locus_tag="OQM83_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCS1518954.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(5977..6580)
                     /locus_tag="OQM83_000603"
                     /pseudo
     CDS             complement(5977..6580)
                     /locus_tag="OQM83_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            complement(6567..7448)
                     /locus_tag="OQM83_000604"
     CDS             complement(6567..7448)
                     /locus_tag="OQM83_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCS1518955.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(7469..8779)
                     /locus_tag="OQM83_000605"
     CDS             complement(7469..8779)
                     /locus_tag="OQM83_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1518956.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(8814..10324)
                     /locus_tag="OQM83_000606"
                     /pseudo
     CDS             complement(8814..10324)
                     /locus_tag="OQM83_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
     gene            complement(10536..10850)
                     /gene="pspE"
                     /locus_tag="OQM83_000607"
     CDS             complement(10536..10850)
                     /gene="pspE"
                     /locus_tag="OQM83_000607"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCS1518957.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(10925..11146)
                     /gene="pspD"
                     /locus_tag="OQM83_000608"
     CDS             complement(10925..11146)
                     /gene="pspD"
                     /locus_tag="OQM83_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCS1518958.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(11155..11514)
                     /gene="pspC"
                     /locus_tag="OQM83_000609"
     CDS             complement(11155..11514)
                     /gene="pspC"
                     /locus_tag="OQM83_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCS1518959.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMLDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(11514..11738)
                     /gene="pspB"
                     /locus_tag="OQM83_000610"
     CDS             complement(11514..11738)
                     /gene="pspB"
                     /locus_tag="OQM83_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCS1518960.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(11792..12460)
                     /gene="pspA"
                     /locus_tag="OQM83_000611"
     CDS             complement(11792..12460)
                     /gene="pspA"
                     /locus_tag="OQM83_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCS1518961.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVNDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            12627..13604
                     /gene="pspF"
                     /locus_tag="OQM83_000612"
     CDS             12627..13604
                     /gene="pspF"
                     /locus_tag="OQM83_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCS1518962.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(13722..14987)
                     /gene="puuE"
                     /locus_tag="OQM83_000613"
                     /pseudo
     CDS             complement(13722..14987)
                     /gene="puuE"
                     /locus_tag="OQM83_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15025..16305)
                     /gene="puuB"
                     /locus_tag="OQM83_000614"
     CDS             complement(15025..16305)
                     /gene="puuB"
                     /locus_tag="OQM83_000614"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCS1518963.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(16307..17797)
                     /gene="puuC"
                     /locus_tag="OQM83_000615"
                     /pseudo
     CDS             complement(16307..17797)
                     /gene="puuC"
                     /locus_tag="OQM83_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(17947..18504)
                     /gene="puuR"
                     /locus_tag="OQM83_000616"
     CDS             complement(17947..18504)
                     /gene="puuR"
                     /locus_tag="OQM83_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCS1518964.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(18531..19295)
                     /gene="puuD"
                     /locus_tag="OQM83_000617"
     CDS             complement(18531..19295)
                     /gene="puuD"
                     /locus_tag="OQM83_000617"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCS1518965.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            19507..20868
                     /gene="puuA"
                     /locus_tag="OQM83_000618"
     CDS             19507..20868
                     /gene="puuA"
                     /locus_tag="OQM83_000618"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCS1518966.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21017..21181
                     /locus_tag="OQM83_000619"
     CDS             21017..21181
                     /locus_tag="OQM83_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1518967.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            21171..22556
                     /gene="puuP"
                     /locus_tag="OQM83_000620"
     CDS             21171..22556
                     /gene="puuP"
                     /locus_tag="OQM83_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCS1518968.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            22690..22935
                     /locus_tag="OQM83_000621"
     CDS             22690..22935
                     /locus_tag="OQM83_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCS1518969.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            23248..24891
                     /gene="sapA"
                     /locus_tag="OQM83_000622"
     CDS             23248..24891
                     /gene="sapA"
                     /locus_tag="OQM83_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCS1518970.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            24888..25853
                     /gene="sapB"
                     /locus_tag="OQM83_000623"
     CDS             24888..25853
                     /gene="sapB"
                     /locus_tag="OQM83_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCS1518971.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            25840..26730
                     /gene="sapC"
                     /locus_tag="OQM83_000624"
     CDS             25840..26730
                     /gene="sapC"
                     /locus_tag="OQM83_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCS1518972.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            26730..27722
                     /gene="sapD"
                     /locus_tag="OQM83_000625"
     CDS             26730..27722
                     /gene="sapD"
                     /locus_tag="OQM83_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCS1518973.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            27724..28530
                     /gene="sapF"
                     /locus_tag="OQM83_000626"
     CDS             27724..28530
                     /gene="sapF"
                     /locus_tag="OQM83_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCS1518974.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            28598..28951
                     /locus_tag="OQM83_000627"
     CDS             28598..28951
                     /locus_tag="OQM83_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCS1518975.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            29319..30107
                     /gene="fabI"
                     /locus_tag="OQM83_000628"
     CDS             29319..30107
                     /gene="fabI"
                     /locus_tag="OQM83_000628"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCS1518976.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            30252..31378
                     /locus_tag="OQM83_000629"
                     /pseudo
     CDS             30252..31378
                     /locus_tag="OQM83_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            31446..33380
                     /gene="rnb"
                     /locus_tag="OQM83_000630"
     CDS             31446..33380
                     /gene="rnb"
                     /locus_tag="OQM83_000630"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCS1518977.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAKKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(33458..33561)
                     /locus_tag="OQM83_000631"
                     /pseudo
     CDS             complement(33458..33561)
                     /locus_tag="OQM83_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            33615..35605
                     /gene="pdeR"
                     /locus_tag="OQM83_000632"
                     /pseudo
     CDS             33615..35605
                     /gene="pdeR"
                     /locus_tag="OQM83_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            35752..35925
                     /locus_tag="OQM83_000633"
     CDS             35752..35925
                     /locus_tag="OQM83_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1518978.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36015..36764
                     /locus_tag="OQM83_000634"
     CDS             36015..36764
                     /locus_tag="OQM83_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1518979.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37033..37251
                     /gene="osmB"
                     /locus_tag="OQM83_000635"
     CDS             37033..37251
                     /gene="osmB"
                     /locus_tag="OQM83_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCS1518980.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(37377..37703)
                     /gene="yciH"
                     /locus_tag="OQM83_000636"
     CDS             complement(37377..37703)
                     /gene="yciH"
                     /locus_tag="OQM83_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCS1518981.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(37703..38440)
                     /gene="pyrF"
                     /locus_tag="OQM83_000637"
     CDS             complement(37703..38440)
                     /gene="pyrF"
                     /locus_tag="OQM83_000637"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCS1518982.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(38633..39802)
                     /gene="lapB"
                     /locus_tag="OQM83_000638"
     CDS             complement(38633..39802)
                     /gene="lapB"
                     /locus_tag="OQM83_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCS1518983.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(39809..40117)
                     /gene="lapA"
                     /locus_tag="OQM83_000639"
     CDS             complement(39809..40117)
                     /gene="lapA"
                     /locus_tag="OQM83_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCS1518984.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(40266..41111)
                     /gene="pgpB"
                     /locus_tag="OQM83_000640"
     CDS             complement(40266..41111)
                     /gene="pgpB"
                     /locus_tag="OQM83_000640"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCS1518985.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFCIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAGHTMFAASWALLAVGLLWPRRRTLTIAILL
                     VWATGVMGSRLLLGMHWPRDLVVATLISWALVAVATWLAQRICGPLTPPAEENREIAQ
                     REQES"
     gene            41281..41871
                     /gene="ribA"
                     /locus_tag="OQM83_000641"
     CDS             41281..41871
                     /gene="ribA"
                     /locus_tag="OQM83_000641"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCS1518986.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(41935..44610)
                     /gene="acnA"
                     /locus_tag="OQM83_000642"
     CDS             complement(41935..44610)
                     /gene="acnA"
                     /locus_tag="OQM83_000642"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCS1518987.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKV
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(44774..44869)
                     /locus_tag="OQM83_000643"
     CDS             complement(44774..44869)
                     /locus_tag="OQM83_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1518988.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(44983..45150)
                     /locus_tag="OQM83_000644"
     CDS             complement(44983..45150)
                     /locus_tag="OQM83_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1518989.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(45153..45317)
                     /locus_tag="OQM83_000645"
     CDS             complement(45153..45317)
                     /locus_tag="OQM83_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCS1518990.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
BASE COUNT        10739 a        11975 c        11433 g        11235 t
ORIGIN      
        1 ttatcggcaa tttccaggtt gaagtccttc accacatgca cctggttatc gtagattttt
       61 tgaatatgtt gtaacgaaag ctgagccatg ttgattcctt tgctatacgt tttgccagaa
      121 ggccgataac cgcgaccagg tcagggagtc cgttgaaggc aatagtaatt gcgccccggt
      181 tatgcccgcg ccgatcccca ccgagcgcat accgctggcg ttaatggcgt caatgcccgc
      241 ctgcgcatct tcaatgccga tacatgcctg cggcggcacg cccagcccgg cacaggcagc
      301 gagaaagatt tccggatccg gtttcgagtt tttaagttgg gaagcatccg cgcagaaggt
      361 gaaaaactca cgcagctcca gtgcatctaa aatcgtcggc gcattgaggg agacagaagc
      421 cagccctacc gagatctgtt gtgcgcggag atctgccagc aaagagcgaa tgccgggtag
      481 aacagcgttg accgtcaact cgcgcaatga gtggacatag agcagatttt tgcgatacgc
      541 cagttgcgcc ctctcctgcg agttaaagtc gccctctttg cccccgtgtt gcagaatgcg
      601 ccgcagagac tcatcgcggc tgatcccttt tagggattcg ttaaactgcg catcaatgct
      661 gatgccaatt tcagcggcaa tctgctgcca cgcctggaaa tgcagatgcg cggtatcggt
      721 gattacacca tccagatcga aaattacctc ttgcagtttc attcatcctc ctgatgtgtg
      781 gcagcggtcc cattgaaggg caaaataaaa tcacccaaac agaaaacaga ttcttcagcc
      841 acggatataa gctgaccgtt caaacgcagt gaaacggggg cagaagttcg aatcgcaata
      901 cgctgcgcgt caagagtgac ctgtaattcg cagccctgcc agaacagagg gaaagacaac
      961 tgttgccact gctcaggtaa cgccggattg agatgcaatt caccgtcacg cacgctcacc
     1021 ccggcaaaac cctgaatcgc ccccagccag atagcgccag ttgcggcagc atggatacca
     1081 tcatcacaac tatgcggatc agcaccaaga tcgatttcag tcccctcgcg ccagaactga
     1141 taactttggg tcagcaggcc acagcgtgcg gcaacaatgc cgtggattgc tttacttaat
     1201 gacgagtcgt gaatagtgcg cggttcataa aattgcagat tggcaagaca cgatgccgct
     1261 gagaactgct ccggcagcat gtaattgagc atcaccacat cagcttgttt gaggatttgc
     1321 atctcgttca cttctgcgcg tgaataatcc agcagaatgg tttgcttccc cgccgccgct
     1381 ttgtatttcg caagattaat cgccggctta gccataaacg aatcatcctg cggcaaaacg
     1441 ccgtcgggct gaatttctgg cagccgcagt tctttaagga acatttcggc acgatgaata
     1501 aacgcgtcgt cgctacagcc aaactggcgg gcaatactca gcgcctgctg gacgttgtaa
     1561 tacgccatat agctggtgaa ggcgttatta ttgacatgtt cggtatattc gtcaggccca
     1621 ataacatcat gaatttccag acggtcgtta accctcaccg cgcggctaat ccagaacttt
     1681 gcagtttcca gaagtagcgc catgccttca tgcgcaatga aactttcatc ccccgtggtc
     1741 tgccagtatt gaataaccgc ccaggcgata tcggccacca gatgatgttc cgcctgcgcc
     1801 gaggccactt tttgccgcag cccggtgcga atgttaatgg cggcaaattc cggcgtctct
     1861 tcttcgccgc tgcgcgcgct ttcccacgga aatagcgcgc cctgccagcc gttgcgtcgc
     1921 gctttctcct gcgcgcctgg caagttgtgc cagcgataac gcagtaaact tcgggcaacc
     1981 gtcggatcgc taaacagatg aaacggtaac aaaaatactt ctgtatccca gaaaacgtgg
     2041 cctttgtagc cttcgccggt taagcctttt gccgcaatgc tgctgcgctc gtcgtgggca
     2101 ggcgtcatga tgcgcagatg ataaagcgca taatctaacg cttgctgatc gtgcacttcg
     2161 ccgccattta ccgtgatacg acgtttctgc caccattgac gccagttttc tgttgatact
     2221 gcaagaagtt ggtcgtaact ctgctgcgcg cacatttcaa gctggcgaag cgacgcgctg
     2281 ccccactcgt ctaaaacggc ttgcctgtca tcccgccagt cgatccacac cagtttttgc
     2341 aacgtcactg tctcgcctgc atgaagctgc gcactggtat gttgcagtaa acggcgctct
     2401 ttggcggtat agcattgctg cacatcaccg ctcaccttac agcaacagct gatggccaca
     2461 tcactgcgtc catcctgggt ggtgtagatc ccctgcatca aatgctgacc aaacacccgc
     2521 acctgggttt cgtcgagatg ctggcgaccg tggttggtct gcgtggcgtc aatgcctgtt
     2581 gaaatcagca ctgacgcgtc ggcgtccagt ggcgtaatag tgatttccag cacaatgagc
     2641 ggcagttggt ctgccgaaac aaagcgacgg ctggcgatgg tgtaacctgt gccgttgctg
     2701 gtacgccaga caacgctacg gcgtaattct ccactggcaa agtcaagttc acgctgccag
     2761 gcttcgcggg ataacgagaa aacctcacca tttatggcaa tctccatccc cacgacatca
     2821 ggcaggttca ccagttcgtt gatttcacct tttcccgccc gatgatacag ccccgccaga
     2881 tacatccctc gcgtctggcg cgtgtaatct tcttcatggc tggcgcgaag cccaagatag
     2941 ccgttcccct gcgccatcag cgatgcatac ttgttcaggg tatgctggct gaaatggggt
     3001 tctgataacg ttactggcct ggtcataatt ccacacgcgt ccctgtttct gcggattgat
     3061 acaacgccgc caccagttgc tggatgatgt acccctgctt ggcgtcggct atcatcacgg
     3121 gcttgccctg tacgtgattg ataaaggctt ccatgctgcg caaatggcgg ttgtcgtctg
     3181 ccattttccg ttgcatcagc gtcattaatt caccgttgtt atcggtgtag atatgtgctg
     3241 gaaacagcgt cgcaccagct ttatcaccac aaaagctgac gttcataatc gactgttcgc
     3301 ggatgttgag tgcaaatgac gtttccagcc acagaatgcc gccgttatga aattcaatgg
     3361 tgccaaacag cgaatcttcg acgctgtaag ttgccggatc ccactcacca aattgaccac
     3421 agctcttttg cgtgccgatc ttttgaaagc tatgcgcatt cacgcttttc accgccggaa
     3481 agcccagcac atacatcgca gcatccagca tatgaatgcc gatgtcgatc agcgggccac
     3541 caccctgcag ttctttattg gtaaagacac cccagccggg aacgccgcag cgacgcaggg
     3601 cgcgggcggt ggtaacgtaa atctctccca aaacgccgtt ggtcacctgt tcacgcagct
     3661 gttgcgtatc gagcgcaaaa cgatggtgaa agtcgtaggc cagcaccttg cccagtttgc
     3721 gcgcggtatc gcacatttcc cgcgcctgtt ctggcgtcat ggcgagcggt ttttcgcaca
     3781 tcacatggca gcccgcttcc agtgccatca gggtatgttc gtaatgaaaa cggttaggtg
     3841 agcagacgct aaccacatca ggtttcaccg ccagcagcat ggcctgcgga tcgtcccaca
     3901 cggatgcatt cccgtatttt tctgccagcg cctgcgcctg ggaaaggcgg ctgtcacaga
     3961 cagccaccag ttccagatcg ttgcgggtgc agtagtacga agcatgaacc ttatccgcca
     4021 cctggcctgc gcctattatc gcgacccgca gcggagagct tgtcattgca cttttcacaa
     4081 agacctctta gcaggtacgc aaccaggcca acgaatcacg gtacgcctgg gcaggatctt
     4141 ccgcccggat acgcccttcg tacactacat agccctgata gttatccgcg cgcagctgtt
     4201 caaacagcgc gtggaaatcc agggtgccgc tgcccggctg gtagcgatgg ttatccgcaa
     4261 tatgcacatg accgagcagg tcgcggttgt catgcagcgc ctgcgccagg ttatcttctt
     4321 cgatattcat gtgatagaaa tcgccgataa tctgtacatg tttaagatcg ttttcgacga
     4381 tgtaacggcg ggcatcggcg agggtgttga tcatatgatc ctgatagcgg tttaacggtt
     4441 cgagatacac cactgttccg gtacgcgcgg cgacctgttc cagtacgcgc agggaatcac
     4501 tcaccatttt gcggtcgccg tccaggctac gcggcgaggt catcggcggt aagcggaagg
     4561 taaacatgcc ccacgctgcc ggaacgacga tacctttatc ccccacttct gccagcgctt
     4621 cgagaatgcg ttcgatctgc tttaagccat taagacgacg ctcttcgata aagtcgccaa
     4681 tccaaccgtc atagccaccg caggcggtgg tcaccggtaa accggtttct ttgattgctg
     4741 ctttgacttc ttcgagattg ttgaccagca gtttgccgtc aatctcaaaa ccatcgaagc
     4801 ccatctcttt gatataacga aatttctcca gaatgttttc cggaaagaat gcctgattct
     4861 gtgtgcctat tttcattttt tcattccttt gaattaaaac gtaacgccca ttttgatgct
     4921 ctgttccgga tgctgatcga catactgcat gtagctttcc gggctattgg caaaggtcac
     4981 taccgggtcg attaaatctt cgcaattgag ataaccgttc atcagcagtt cccagcaggt
     5041 ttcttcaata cgcttacggc tccagcgcgg ataatccggg ttcggttcgc tgcacgcgcg
     5101 ggagaaaaca attttggcgt tattgaaatg cgcttcgcgt ccgaggttaa aaccttcagc
     5161 aaatggtttg gcaaacgcaa cataggagat ggtgccaccg taggccagac cgcggaacgc
     5221 cgattgcagc gcgtcggcat agccgctggt ttcgataatc acatcggcac cctgcttgcc
     5281 ggtcagcgtt ttgatctctt tgcctacgtc agtaccaatg gggttaaggc agaaatccgc
     5341 gccgtggcga cgggcaatat cacagcgatg agcgatagga tcgacgccaa tcacgaccga
     5401 cgcgccagca cgtttagcca gttgaatggc aatttgacca atcgcgccaa gccctaccac
     5461 cacaacaaaa tcacccacgc gcacgttggc atcgcgcacg ccgctcatgg caaactgcgc
     5521 cgggtcgtaa caaacggcgt ttttccacga gctgccttcc ggcattttgc gcagcttgta
     5581 gttattcacc gcgttaatga tgaccgtctc ggagagcggg ccgtagccgc atacgctgtc
     5641 gcccaccgcg tagtcggtaa cgtcgctgcc gcactcgata atgtcgccaa ccaccatgtt
     5701 tccaagctgg aatttgccaa actcaatgcc gcgcggcgca tctgccggac gcggagtgaa
     5761 catctgccat tcgccgttaa agtcttcatc gataaacggg ctggcggcgc ggaagtcgac
     5821 cacttccgtt ccgtgtttcg gtgcgccgaa acgcacgcgg accttcactt cattagctaa
     5881 aatcgcccga tcttcatact caaccagcgc agcaacacgc ggtgctgtgg ctactaactt
     5941 tttcatttaa aactccttgt aaagaaactg gccgctttag cccttcacgc caccggcggt
     6001 caaaccactc ttaataaaac gttccgaaag cgcatacata atcaccaccg gcaatgcggt
     6061 caccagtgag gccgccatca tccgtcccca gatgtaatct ggcgtactga acagcgcgtt
     6121 caggcccacc ggtaaggtga aattgccggc gctggagagg aaaatcgacg caaacagata
     6181 gtcgttccac gccaccataa agcagtagac gaacacggat atcagcccgg acatcgccaa
     6241 cggcacggtg atgcggaaga taatttgcag acggttgagg ccatccatca tcgccgcttc
     6301 ttcgatctca tcggggatgg tgtcgaagta gcttttcagc ataaacacgg cggtgggcag
     6361 agtttgcgtc accatggtga tgatcagcgc catctcagtc gacgtaatgc tccagcgtcc
     6421 actgctgcgg cagcagcgta ggatgcagtg agatcgcctc tttcgcgccc ttgaacgagg
     6481 tcatcagcat cacaaaaaat ggaaacagcg tgatgatgag aaacagcgcc agcccgcagt
     6541 aaaaaccgat gcgactgagt gtgcgtttat ttgttgccat tgaggttcac ccgtttcctg
     6601 gtcagcagaa tgacagcgaa aataatgatg aagagcacca ccgaaatcgc cgccgctttc
     6661 cctaagtcat taaaggcaaa ggcggttttg tagagataga cgccgagaat atcgaccttg
     6721 gtggtcagca gataaacatc agcaaacatg tagaacatcc agatagtgcg cagggtcacc
     6781 accgtcgcca ggaccggcat aattgcgggc agcgtgacga tgcgaaaccg ctgccaggca
     6841 ttagcgccat ccatctctgc cgcttcgtac agcgatttgt cgatggtctg caaaatcgcc
     6901 agaaacgaga taaaggcata cgggaagtag cgccagatgg cgaacagcac caccagcaca
     6961 aagctactgc ccggattatc gaaccacagc ggtgcctgct catagagatg cagaagatcg
     7021 acgccgaggt agttcacaat gccgtagccg ttgttgaaca tgtatttcca ggcaaacacc
     7081 agcgaaatgg acggcgttac gtaggagagg atcaccagcg aacgcgcggt tttgcgcagg
     7141 cggaactcgc ggttgaaaaa catcgccact gccagcccga gcacggtgct gcccgccacc
     7201 accagcgcgg tataccagac cgtcatccac agcgagtgcc agaagccggg atcggagagg
     7261 atacgcacat agttactcac cccaacaaag gttgactcga tgttgggatt gagcggcaga
     7321 cgtaaaaaac tgatttcgat attcgacacc attggccagg ccaccagacc acccagcagt
     7381 aataagctgg gcgcgagaag cagcagcgca aagggcatat cggaacgacc tgaaaaaagc
     7441 ctgttcattg cggattcctg tcctgggttt agtgctgttc gatcagttca tccagctttt
     7501 tctggctcgc ttgcagcgtg ccagggagat cggctttacc cacggtgacg ttatgcacca
     7561 tggagctcac cacgccagaa cccgtcacat cacccatgcg ggtaaagttt ttatccccta
     7621 ctgcgccaaa gacctgaata tttggcagtt caccgattag ctgattcggt agttcaccca
     7681 gcgccttaat aacgtcgttg tctttccagg tggcggtagt gacaaccgct ttattcaccg
     7741 gcagcgcagc acctggcgac atcatcaccc agtcggcaat gttgtctgcc tgctccataa
     7801 aggtgacaaa tttttctgct gcttccgtct cttcggcctt ttgcccggcg gtaatggtca
     7861 gcgaggtcaa catgccgtag accgcagagt ttttctcggt tggcaccacg aaaccgacgt
     7921 ttttcgggtc gccttcttta attacagccg gaaggatata ggtggagtaa atcgccatcg
     7981 gcgcggtgcc gttcataaag gcgtctttca cttccatgat gtcgttagaa cccggcatgt
     8041 cgttagaacc cggcatagtg ttggcagcaa ggtcgcggta ataggtcagt gcctgcatca
     8101 tctctggtgt atcaagggtg attttgcctt cggcgttaaa gacattagcc tggttggata
     8161 acgcaaactg ggagaaggat tgttccgtca acacgctttc tgctgtaggc agcgcaatgc
     8221 cgtatttttt attcgccggg tcattcagtt tctgtgcaac gtccagtagc tgttgccagt
     8281 ttttcggctc ctccagcccc gcttttgcca gcacatcttt gcgataccag atgccgccaa
     8341 tccaggcgct gacgggaaca ccggtccagg cactcccatc ttcggtgcgc acaatacgca
     8401 gtacgccatc gtaaaacgcg ccttcaccaa cgttgctgat gaccgtggca accgtcttgc
     8461 gatcgataag ctgctcttta tccataactt tggcgtagtc atggctggtt tcgatcactt
     8521 ccggcagcga gccgctacgt gaaagggtga tgactttggt gttataagcg tcctcttcca
     8581 cgggcacttg tttaacggtg atacctgggt tttctttttc aaaacgggca atcaatttgc
     8641 tgataactgc ctgacgctcc tgctccaccg aagaatgcat aaattcgatg gatacatggg
     8701 ttttattttc ttctttacag cctgaaatca gggcgcagga aaccagtgct gataacagca
     8761 cgatttttga tttaatcatg agatgttcct ttttatttaa tccacataac ctgttagggg
     8821 cgcagcgtta tttcattacc caatagatca acttcactaa tcagatcccg accatgcaga
     8881 ttagtaatat ttacatgctg aatattttta ctgacattaa acaatccagt cagacaatca
     8941 ccatcagcgt tacttctttg aatacgcatt acggatgaat taatcgtatc gatggtaaaa
     9001 ttattatccg gatgaaactc gttatggctg cgacgaagtg taattaaacg cgacaactca
     9061 tgatataccg catgccttaa tgtagcttca tcgttcagtt ctcgggttat ctctttactg
     9121 tgatattttt tacggttaat cgcacggtta tatccgagtt tttcgacacc tgcgtaatca
     9181 ttacgcgagc caagaatact ttgaatatat atcgctggca caccggggaa acttaacaaa
     9241 atcgcatggg cgagaataaa cctggcgcaa cgttcttcat cgctactctc acggcggctt
     9301 aacgcatcca tatgtgagag gcgaggaagt taaaccaggt ggtgttgctg gaaggtagtg
     9361 tcaggttttg cgcccacgca caaagcgcct caacgttctg tttttgcacc gcatgcagca
     9421 ccagtggcgg cagcgagaac tggtacacca tatgtgcttc gtcatcgcct gcgccaaagt
     9481 aagcaatgtt gtctttatgc ggaacattgg tctcggtaat gatcactgta cctggcgcaa
     9541 cgttatcaat aatcgaccgt aacagtttga taatcagatg tgttttttcc agatggatgc
     9601 agcttgttcc cggctctttc cacataaagc caacggcatc caggcggaca tactcagcac
     9661 ctttcgcaag gtaacagagt aaaacatcca ccatcgccag caacacttca gggctacggt
     9721 aattcaggtc aatttgatcg tcactaaagg tggtccataa atggcgcgtt gaatgatcgc
     9781 gcatctggaa tggcgttaat aacggtaacg cacgcgggcg agtgacggcg ctgagatcgg
     9841 tttgcggatc aaccgcaata aaaaaatctt caaaacctgg ctgctgttgt aaatagtttt
     9901 taaaccattc actttttgcc gacatatggt tgcagacaaa atcaaacatt aaatgactgc
     9961 attcaccgag ttgctgaata tcctgccact cccccgcttc actggcgacc tgatgataat
    10021 caattaccga aaacccatca tcagatgaac atggataaaa aggtaataaa tgaacatgtg
    10081 aaaaaatgct ttgcagccat tgatggtaaa actgattaaa tgtgggtaat ggttttaaat
    10141 cattgctgtg aaattgatcg gcataggtaa ttaacacgac atcactttca tcccagtgtt
    10201 ttttacgtcg ctgtgtaatt aatcgtttcg cactctcaag acgcgttaca agtttctgta
    10261 aatgagttgc ggtaaatgtt ccaccgtaga tttcgtccag gtaatccgta attttctgtt
    10321 tcatgttgcc tttttgtggt agcggtccca tgaaacgaga gactactccg cagaaaataa
    10381 actgtcaatt gagcacaagg gttttagtca cgcaggaaat cattttttac cagggaaaaa
    10441 gcgtatttgt gagctgacgc aaataatcta tggggatgtg aataaggtgt gacagaaaaa
    10501 aaacggcgca taagcgccgc tcatggtgaa ttcttttaac ctttgacctt cggcattgcg
    10561 atgtctttca ggccaccggc gttctcaacg tgggtatatc ccatctcgct aaggatctct
    10621 tttgcttgcc ctgactggcg tccggcattg caatacactt tcacggtgtc gtttttatcc
    10681 ggaacggcgg tggcaatgcg ctctttcact tccttcaggg gaatattgat ggccccctga
    10741 acgtgctctt gctgatactg ctctggaaca cgaacatcga tccagtgttc agcggcgaaa
    10801 acgggcagtg aaaacaccag tgccagagca agtaagcctt ttttaaacat gtaagcgtcc
    10861 ttctatggat gaaaattgcc atccattata cgcctgagat ttaacaattt ttgcgaaact
    10921 cccctcacct tttataacgc tgtgccagtt tattagcagc ccgacttaac agcggttcca
    10981 gcgccacagc cagcaacatt ttcagcggtc ggcgagcaac tgattttatc gcccagcccg
    11041 ctacacccgc cgggccatag cgcagtgcgg taagaagtac cagcttgcct gctaatttga
    11101 aaccaggctt taccttttgc ccggcctgtt gccagcgagt attcataact ttcctcacag
    11161 ttgacggaaa cggctacgta acgtgaaagt atcggaagtg acataacgtt ccatctcgcg
    11221 taaacgcgtt tcacttgccg ccagttcacg gtcgacttca tccagcaatt cgctgctgga
    11281 aggtagctgc tcaccaaagg ccatgttgtc cagcattgga tcaagcgcaa atgacaaaat
    11341 gatgtaagca accagggtaa acagcgccag accgaagaaa atcgacagca ccaccaggat
    11401 acgcaccagt tttaccggta catcaaaata gttggcaatc ccggcgcaga cgccgcggac
    11461 catgccctgc tgtggaatac gccataattt tttattgaga ttaatgcccg ccattagcga
    11521 tccctccagt tcggatgttc tgcatcaaga atagattcca gcgcctgaat acgttcgcgc
    11581 atccgttttg cttcatcagc cagttgcgct aatcgctgct gctcactttg cgacaattca
    11641 tcgcgaccag aacgattgct gtaatgcagc cataaccaga tcggtaaaac aaacagcaca
    11701 aaaatggtta acggaatagc cagaaatagc gcgctcataa gtactcctta gacgatgagc
    11761 ggcggcgcgt tcggacgccg cctggatgtt attattgatt gtcttgcttc attttggctt
    11821 ttaattgtgc cagttgttcg ctgattgcat catcggcttt cagttcggca aactgatcgt
    11881 ccagcgattt ttgtttaccg aagctgtggc tttctgcttc cgcttccatc tggtcaattc
    11941 gacgttcgaa agattcgaaa cgagccattg cttcatccag tttgccactg tccagctgac
    12001 gacgcacatc gcgcgacgag tttgccgcct gatgacgtaa catcaatgcc tgctggcgag
    12061 cgcgtgtttc gctcaatttg ttttccagct cgccaatctc tttcttcatg cgtgccagcg
    12121 tatcgttcac cagcgtcact tcatgttcca gggacttaat cagatcggtc agtttctgtt
    12181 tttcaattaa cgctgcacgt gccagatcct ctctctcttt cagcagcgcc agttcggctt
    12241 tttcctgcca ttcaacttca cgcgccgacg cttgttcaat acggcgagtc agctgtttct
    12301 tttctgccaa cgcacgcgcc gaagtagaac gtacttcaac cagtgtatct tccatctcct
    12361 ggatcatcag acgcaccagt ttctgtggat cttccgcttt ctctaacaga gcgttgatgt
    12421 tggcattcac gatgtcggca aagcgagaaa aaatacccat aatgttgtcc tcttgatttc
    12481 tgcgttcagg attgtcctgc tgaactgtta atacaatttg cgtgccaatt ttttatcttt
    12541 ttgatttata aagatctgat tgaagaatca acagcaacat gccaggatga attagcgaat
    12601 tacactaaca agtggcgaat ttcatcatgg ccgaatacaa agataattta cttggtgagg
    12661 cgaacagctt tctcgaagtg ctggaacagg tttcgcatct cgcaccgctg gacaaaccgg
    12721 tgctcatcat cggcgaacgc ggcaccggta aagagctgat tgccagccgc ctgcattatc
    12781 tctcctcccg ttggcaaggg ccgtttattt cccttaactg cgcggcgtta aatgaaaatc
    12841 tgctggattc cgaactgttt ggtcacgaag cgggggcgtt taccggtgcg caaaaacgtc
    12901 atccagggag atttgaacgt gccgacggcg gtacgctatt tcttgatgaa ctcgctacgg
    12961 caccgatgat ggtgcaggag aaattattgc gcgtgattga gtacggtgaa ctggagcgcg
    13021 ttggcggcag ccagccattg caggtgaatg tgcggttggt atgcgcgacg aatgccgatc
    13081 tcccggcgat ggtcaatgaa ggcacttttc gcgctgacct gctcgaccga ctggcttttg
    13141 atgttgtaca actgccacca ctgcgcgagc gcgaaagcga cataatgttg atggcagaac
    13201 actttgccat ccagatgtgt cgggaaatca agctgcctct gttcccgggg tttacggagc
    13261 gcgccagaga aacattgctg aattatcgtt ggccgggaaa tattcgtgaa ttgaaaaacg
    13321 cggtggaacg ttcagtgtat cgccacggca ccagcgatta tccgcttgat gacatcatta
    13381 ttgatccctt taaacggcgt ccgcctgaag acgctatcgc cgtttcagaa accacctcgc
    13441 ttccgacgct gccgctggat ttacgtgagt ttcaaatgca gcaggaaaaa gagttgctgc
    13501 aactcagttt gcaaaagggg aaatataacc agaagcgcgc agctgaattg ctggggttaa
    13561 cctaccatca gttccgcgcg ttgttgaaaa aacaccagat ttagcgaagt tgggcaatgc
    13621 gattcgccgg atgcggcgtg aacgctttat ccggcataca taatcaacgt cgtttgcagg
    13681 cctgataagc gcagcgcatc agacattatt gcgttgggct attaatcgct cagcgcatcc
    13741 tgcaaaattt tcattgccgc atcgaattgc gcatccggga tggtcagcgg atacaggaag
    13801 cgaatcacgt tgccgtatgc gccacaggtc agcaggagca gcccctgcgc cagcgcgcgt
    13861 tgctggattt tctgtgcaat cgccgctgac ggctcgcccg tttgcggatc gttaaactct
    13921 gccgcaatca tcgaccccag gccgcgtacc gcagcaatgg ccggaacgct ttctttggca
    13981 tcaatcaacg tgtttttgag acgctggccc agttgattcg cgcgttcgca gagtgattct
    14041 ttgttgataa tattgagcac cgcgtgcgcg gcagccaccg ccagcgggtt cccggcgtag
    14101 gtgccaccca acccgcccgg cgcgggcgcg tccataatat tcgcgttacc gaccacgccc
    14161 gaaagcggca tcccgcccgc gaggcttttc gccatcgtca ttaagtccgg cttatcggcg
    14221 taatgatcca tggcaaacag tttaccggta cgcgcaaagc cgctttgcac ttcatcggca
    14281 atcatcacaa taccgtgctc gtcgcacagg cggcgaatgg cggcaaccag ctcttttggc
    14341 gcaacgttga aaccgccctc gccctgcacc ggttcgaaaa taatggccgc gacctgcttc
    14401 gcttcgatgt ctgatttaaa caagcgttcg atggcgtcga gggagtccta tgttgaaatg
    14461 ccgtgtaaat ctgacggata aggtacgtga tacaccgaac cagggaacgg gccgaagccg
    14521 attttgtacg gcgcaacttt tccggtcagc gccatggtca tatatgtacg accgtggaag
    14581 ccaccgctaa acgcaatcac gccagggcgt ccggtatggg cgcgagcaat tttcaccgcg
    14641 ttttccaccg cttccgcacc ggtagtgaag aacgcggttt tggcctgccc gctcaccggg
    14701 gcaagggcgt tgattttctc cgccagggtg acgtagcttt catacggcac aatctgatac
    14761 gcggtgtggg taaactgttg cagttgctgc tccaccgccg cgaccagatc agggtggcga
    14821 tgtccggtat tcagcaccgc aatgcctgcg gcgaaatcga tgtactcgtt gccctcaaca
    14881 tccttcagag tagcgttttc agccgactgg gcgaagaagt tacacatcac gccaaccccg
    14941 cgtggggtag cagaaagacg acgctgatgg aattcattgt tgctcatgtt tcacaccttt
    15001 tgactcttca ctgttaatcg gaaattaaac gcccagacga tcgcgcaggc tgtaccaggc
    15061 tgcgcccatc gcggtaaacg gtactcgcag cgtgcgcccg ccggggaacg ggtaatacgg
    15121 cagattggcg aaggcatcga aacgttcggc gtcgccgcgc agcagttcgg caatcaaacg
    15181 tccggcgaga tgagtacagg tgacgccgtg gccgctgtaa ccctgcatgt aatagatgtt
    15241 ggtatcaagg cgaccaaact gcggcatgcg cgacagggtc agcaggaagt tgcccgtcca
    15301 gcggtagtcg attttgacgc ccttcagttg cgggaaggtt ttcagcagtt tcggcacaac
    15361 aagacgctca acgtcatccg ggtcgcgcgc gccgtagacc acgccgccgc cgtacagcaa
    15421 acggttgtcg gcggtaagac ggtagtaatc aagcagataa ttacagtctt ccacacagta
    15481 gtttttcggg atcagcgaac gggctaaatc ttccgacagc cgttcagtgg tgatcacctg
    15541 cgtgccgcac ggcatgctgc gtttcgccag ttccggctct actttatcgc ccagatacgc
    15601 attcccggcg acaatcacat acttcgccgt cacctgacct ttggcagtgc gcaccaccgc
    15661 tggcgtggtg tgctggatct gcgtcacggc agaaagctca tacacgcgcc cgccgttcag
    15721 gcggatggca tcggcttcac caatggcgag gtttagcggg tgaatatgcc caccgctgtg
    15781 atccaacagt gcgccagtgt agcgatcgct ggcgacctcg cggcgaatgg cgttagcgtc
    15841 cagcagctcc agctgtttat tgccgtagcg ttcccagttc tctttctgct cttcgagtgt
    15901 ggcgagctgt ttatcattca tcgccacaaa caggccgccg gggcggtagt cgcagtcaat
    15961 ctgatagcgt ttgatacgtt cgcggatgat ctcgccaccc tcgaacatca tgctgccgag
    16021 catacgggcg gtgtccatgc cgtagctttt ttcgatcacg tcgatgtcgc ggctgtagga
    16081 gttcacaagc tgcccgccat tgcgcccgct tgcgccaaag ccgatgcgtg aggcttcgag
    16141 aaccactacg tcaaagcccg cttccgccag atgcagcgcg gaggagagcc cggtatagcc
    16201 gccgccaacc acgcaaacgt cgcaggtgat tgactcattc agtgtgtcga atggtgcata
    16261 tttattcgca ctggcggcgt agtaactgct ggtatgttcg gtcatttcag gcctccaggc
    16321 ttatccagat ggttttcagt tcagtgaatt tttcaagggc atgcagggat ttgtcgcgac
    16381 cgttgccgct ctgcttatag ccgccaaacg gcacggtcat atcgccgtcg ttgtagttat
    16441 tgacgaagac ggaaccggct ttcaggcggc ggctcatacg gtgcgcgcgg gagaggtcgc
    16501 gcgtccatac cgccgcgcca aggccgtact ggctgtcgtt ggcaagctgt agcgcctggt
    16561 cttctgatgt gaaacgcgtg accaccagca ccggaccgaa aatctcttcg cggcttaagg
    16621 acgcattcgg gtcaacatcc acaaagatgg tcgggccgat ggcggcagcc agctcagcgt
    16681 tacgaccatc cagcagcagt tgccctttgc tttcgccttc acgaataaag ctatggaccg
    16741 agtcggcgtg ggcgcaatcg attaaggtgc ccatggtggt tacggtatca agtggatggc
    16801 ccggctgcca gttttgcgcc tgctgtttta acagggctaa gaattcatcg gcgatgctct
    16861 cttccagtaa caaacgcgtt ccggcgatgc acacctggcc ctggttgtag aagatgcctg
    16921 cggcggtggc gcttgccgct ttttgcagat ccggacaatc ggcgaaaacg atgttggcgc
    16981 ttttgccgcc cgcttccagc cagacgcgtt tcatgttgct atcacccgcg tctttcagca
    17041 gttgtttacc cgtacgcgtg gagccggtaa aggcgatggc atcgatatcg ttatgacgcg
    17101 aaagcgcctg cccggcttca tgaccataac cgctcactac gttcagcacg ccatccggca
    17161 gccccgcttc ttcttttgcc agcccggcga gacgaatcgc actgagtggt gatttttcag
    17221 acggttttag aatcacgctg tttcccgccg ccagcgccgg gccgagtttc cagcaagtca
    17281 gcagcagcgg gaagttccac ggcacgatgg cggcaatcac gccgaccggt tcacgcacga
    17341 tcatcgccag ctcatggcta ctggtggtcg ccacttcgcc atacactttg tcgatcgctt
    17401 cggcgtacca gcgaatggcg cgcgccgcgc cgggaatatc atcacgcaga ctgtgacgaa
    17461 tcggtttgcc ggtgtcgaga gtttccagca gtgccagctc ttcggcgtgg gcttccatta
    17521 aatcggcgag tttattcagt accgctttac gtttagccgg agaagagagt gaccagtcgc
    17581 cgcgttcaaa tacgccgcgt gctgcgctca ccgcatggtc gatatcgacg ctcttgccgc
    17641 gggcaatttt cgccagcggt gcctaggtga ccggatcaac ggtttcaaag gtttcatttt
    17701 ccgccgcagc agtatattca ccgttaataa ataagcggtt ttcaatggcg agacttaacg
    17761 ctttatcctg ccagtaagcc agatgatgaa aattcattat gactcctgtt tcacgtctat
    17821 cagatatatg cagtttgtga acgtcagggc gcagagatcc ccgggccgca tgcccgttta
    17881 tttctggcga cattctttat ggattgccgg gcgcattgcg cttacccggc ttacagaaca
    17941 aaaagattaa aacgtggtgg gcgtatgggc gctgataatt cggcaaatac ctgccgacgt
    18001 attactgaaa ctgtgcggga tgccggtatt aatggcataa ctttgccccg cgacgaggtg
    18061 gtaatcctga ccattaatcg tcagaacaat ttcaccttcc agtactgtgc ctatttcctc
    18121 accctgatgc ttaattcttt ccccagtggt tgtgcccggc tggtacgttt caaagatcat
    18181 cgccagcgtg cgattcgggt taccgttatg aaccagcttc attgacacac cctgactgcc
    18241 catctcaatt aagtcgtcct gattaatgac gacctgcggc tcatcaggtt tttccggctc
    18301 ggaaaagaat tccgagagtg acagaccata caccttcagc agcttttgca gcgtactgat
    18361 ggcagggctg actttatctt gttctatcgt actgatagca ctgtgagtca gcccggagag
    18421 ttcggcggca cgacgttgtg aaagcccctg ctgctggcgg atttccgaca agcgttttcc
    18481 tggcgccagt ccctcatcac tcatatttgc atttccttaa actgtagtgg tcagagtcgc
    18541 tgtttttcag cgatatggtg ctgacaagcg gtgataaagc cctcgaacaa tatacgcgaa
    18601 agcgcgtact cgctactgtt ccattccggg tgccactgta cgcccagcgc aaaaggatga
    18661 ttgatgacgc taaccgcctc aactaagcca tccggcgagc gagcttcaac acgcaaccgt
    18721 gggctaacga ccttcgcccc ttgtccatgt agagagttta cccaaaagtt gctacattca
    18781 ggtaacaacg cagacagtaa tcccccctct tcaacctgaa cttcatgcga cggtgcatat
    18841 tgctgttcca ccggcagttc gggatcttcc cgatgttcca gcaattcagg ctgttcgcac
    18901 agcttgcgat gcaacgaacc accggttgcc accaccagtt cttgtaaacc ccggcagatg
    18961 gcgaaaatgg ggatgcgcct ttcgagtgcg gcattaatta tcgccatgct cagaagatca
    19021 cgcccgggat cggcgtcagg ctcatcgccg ttttcaccat ataggtgcgg ctgcacattg
    19081 ctgggactac caggaagata aatgccatcg agtttcggca aaagttgttc aagtaacgac
    19141 ggttccgcca gcgcatgtgg tagcgcaatt ggcaagccgc ctgcgtggat gatggcattc
    19201 aggtactttt cttgcagagt ctgggtcgca tgacccttaa gcctgttcct gcacattacg
    19261 acaccgataa ccggattgtt cattatattt tccatgctca atctcacaaa gtggactaaa
    19321 ttatcgccat tactgcgctt taaatgacct ttatgttcaa tattttttca atctagcagt
    19381 gggttttcat ttttgcaaac tcaatttaac atttgacaaa catttagttt gcatacagat
    19441 tcgaatggtg gtcattatat tttacgcttt gataacgagc ggaaaacaaa ccaaaggcga
    19501 agaatcatgg aaaccaatat cgttgaagta gagaactttg ttcagcagtc agaagagagg
    19561 cggggtagcg cctttacgca ggaagtgaaa cgctacctgg agcgctaccc gaatacgcaa
    19621 tatgttgatg ttttacttac tgatttgaat ggttgcttcc gtggaaaacg tatccctgtt
    19681 tcaagcctga agaagctcga gaaagggtgt tatttcccgg cctcggtatt tgcaatggat
    19741 attctgggca acgtagtaga agaagcaggt ctgggtcagg aaatgggcga gccggatcgt
    19801 acctgtgttc ctgttctcgg ttccttaact ccttctgccg ccgatccaga gtttatcggt
    19861 cagatgctcc tgaccatggt cgatgaagat ggcgctccct ttgacgttga gccgcggaac
    19921 gttcttaacc gcctgtggca gcagctgcgc cagcgcgacg ctgaagggaa tctgcaaccg
    19981 ccctgcgcgc ctggcaccga tgaccgcaat acacaaagcc aggtttactc cgttgataac
    20041 ctcaaccact tcgccgacgt gctcaatgat attgatgaac tggcgcagtt gcagctgatt
    20101 ccggcagatg gcgcggtcgc tgaggcctcg ccgggtcagt ttgaaatcaa cctttaccat
    20161 actgataacg tgctggaagc ctgcgatgat gcgctggcac taaaacgcct ggtgcgtctg
    20221 atggcaggaa agcataagat gcacgccact tttatggcga agccgtatga agagcacgcg
    20281 ggcagcggga tgcatatcca tatcagtatg caaaataatc gtggcgagaa tgtgctttct
    20341 gacacagaag gcgaagattc gccgctgctg aaaaagatgc tcgccgggat gattgacctg
    20401 atgccgtcgt cgatggcgtt gctggcacca aacgtgaact cgtatcgccg cttccagccg
    20461 ggaatgtatg tgccgacgca ggcgtcgtgg ggccataaca accgcaccgt cgccctgcgt
    20521 attccgtgcg gcgaccgtca taatcaccgc gtggaatatc gcgtggcggg tgccgatgcc
    20581 aacccatatc tggtgatggc agcgattttt gccggtattt tgcatggcct tgataacgag
    20641 ctgccgttgc aggaagaagt cgaaggcaac gggctggaac aggaaggctt acccttcccg
    20701 attcgccaga gcgatgccct gggtgagttt atcgagaatg atcacctgcg ccgctattta
    20761 ggcaaacggt tttgccatgt gtatcacgcc tgtaaaaatg atgagctgtt gcagtttgag
    20821 cgcctcatca cagaaacaga aattgagtgg atgttgaaaa acgcgtaatt ccccttgcgg
    20881 ggttgtttca tcggcctgag cggagccagg cctgacattt gctggtaatc cagacatttc
    20941 ccggatgtcg cgttatggag cgcaggcggc aacgggcttc cgttaacgat taagactgtg
    21001 acgaggaaat gagatgatgc cgatgattaa atccccgcat ggcgagggag gctgcgtatg
    21061 cgcccctcct gctactgact ggactccgcc ccctttgctt cccttgctaa acaggtttga
    21121 tttcaggtcg acacgaccgc aaaccttatt acgcagggga ggcagcaatt atggctatta
    21181 attcaccact gaatattgct gcgcaacccg gcaaaacccg tctgcgaaaa tcactgaaat
    21241 tgtggcaggt ggtaatgatg ggtctggcct atctcacgcc gatgaccgta tttgatactt
    21301 ttggtattgt ctccggcatt agcgacggtc acgttccggc gtcctatttg ctggcgctgg
    21361 cgggcgtgct gtttaccgct atcagctacg gcaaactggt tcgccagttt ccggaggccg
    21421 gttcggccta tacctacgcg caaaagtcga ttaacccgca cgtcggattt atggtcggct
    21481 ggtcatcact gctggattat ctctttttgc caatgatcaa cgttttgttg gcgaaaatct
    21541 atctctccgc cctcttcccg gaagtgccgc cgtgggtgtg ggtggtaacc ttcgtcgcca
    21601 ttttaaccgc cgcgaatctg aagagcgtca acctggtcgc taacttcaat accctgtttg
    21661 tactggtgca aatctccatc atggtggtgt ttatcttcct ggtggttcag ggactgcata
    21721 aaggagaagg cgttggcacc gtctggtcac ttcagccgtt tatcagcgag aacgcgcacc
    21781 tgatcccgat tattaccggt gcgacgattg tctgtttctc gttcctcggt ttcgatgcgg
    21841 tgaccacgct ttcggaagag acgccggacg ccgcacgcgt gatcccgaaa gccatcttcc
    21901 tgacggcggt atacggcggc gttatcttta tcgcggcgtc gttctttatg cagctgttct
    21961 ttcccgatat cagccgcttt aaagacccgg acgccgcact gcctgaaatt gcgctgtacg
    22021 tcggcggcaa gctctttcag tcgattttcc tctgcaccac gtttgtgaac acattagcgt
    22081 ctggcctggc ttcacatgcc agcgtgtcgc gtctgctgta tgtgatgggg cgtgacaatg
    22141 tgtttccgga gcgcgtgttt ggctatgtgc acccaaaatg gcggactccg gcactcaacg
    22201 tcattatggt cgggattgtc gcgctgtcgg cgctgttctt tgatttagtc accgcgacag
    22261 cactgattaa cttcggtgct ctggtggcgt ttaccttcgt gaatctgtcg gtgtttaatc
    22321 atttctggcg gcgtaaagga atgaataaaa gctggaagga tcacttccac tatttgctga
    22381 tgccgctggt tggcgcgctg acggtgggtg tgctgtgggt taacctagag tcaacgtcac
    22441 tgacactcgg tctggtatgg gcttcgttag gcggcgcata tttgtggtat ctgatccgcc
    22501 gctatcgcaa agtgccgctg tacgaaggtg acagaacgcc ggtgagcgaa acgtaatagc
    22561 cggatgcgcg gcgcattatc ctgcctgagg gtaacgcgcc gcgccattaa ctataaaaag
    22621 atgtataacc cggatcacga gtgctatcat ccgcccttat gcttagcgca ttaaccccag
    22681 gggcaaataa tgaaccacga tatcccgcta aaatattttg atattgccga tgagtacgcg
    22741 accgaatgcg cagagcctgt cgcagaggct gaacgcacac cgctggccca ctacttccag
    22801 ttactgctca cccgtttgat gaacaatgaa gagatcagcg aagaagccca acatgaaatg
    22861 gctgctgaag cggggattaa tcccgtacgc attgatgaga tcgcagagtt tttgaatcaa
    22921 tggggtaatg agtaattatt taattgcaga cgatcggtca gtatcgttgt ccggataaag
    22981 cgttcacgcc gcatccggct aacaatgaca cgcactgcag cgtgctttaa tgggtttctc
    23041 acgatcgtac cgctctgctc ctcgctaagt tgttctgtga gttatagtta tagagaaatc
    23101 acgggtacga atgttgaaca gatggcgacg ccacctgttc aacgaccaac cacacggccc
    23161 tcattacgac ccctgaacac aaacggtaat ggcaaatgtc attaaatatt agtaagatag
    23221 cctctttaca taaaatccct taatattatg cgccaggtat tatcgtctct tttggtgatt
    23281 gctggacttg tgagtggtca ggcaatcgcc gcgcctgaat cccccccgca tgctgatatc
    23341 cgcgacagcg gttttgtcta ttgcgtcagc gggcaagtca acacctttaa cccatccaaa
    23401 gcgagcagtg ggttaattgt cgataccctt gccgcccagt tttatgatcg actgctggat
    23461 gtcgatccct atacctatcg cctgatgccg gaacttgccg aaagctggga agtactcgac
    23521 aacggcgcga cctatcgctt ccacctgcgt cgcgatgttc cgtttcaaaa aaccgactgg
    23581 tttactccca ctcgtaaaat gaatgccgac gatgtggtgt ttaccttcca gcgaattttt
    23641 gaccgcaaca acccgtggca taacgtcaac ggcagcaact tcccctactt cgacagcctg
    23701 caatttgccg ataacgtgaa aagcgtccgc aaactggata atcataccgt tgagttccga
    23761 ctcgcccagc cggatgcttc ttttttgtgg cacctcgcaa cccattatgc ttcggtcatg
    23821 tcggcagaat atgcccggaa gttagagaaa gaagatcgcc aggagcaact cgaccgtcaa
    23881 ccggtcggca ccggtccgta tcagttgtcg gaataccgcg ccgggcaatt tattcgccta
    23941 caacgtcatg atgacttctg gcgcggtaaa ccgttaatgc cgcaggtagt ggtggattta
    24001 ggctccggcg gcaccggacg tctgtcgaaa ctcctgaccg gggaatgcga cgttctggcc
    24061 tggcctgctg ccagccagct atccattttg cgtgacgacc cgcgcttgcg tttaacgctg
    24121 cgtcctggga tgaacgtcgc ctatctggca tttaacaccg ccaaaccgcc gctaaataat
    24181 cccgctgtcc gccatgcact ggcactggcg attaataacc agcgcctgat gcaatccatc
    24241 tattatggta cggctgaaac ggcggcctct attttaccgc gcgcctcgtg ggcctatgac
    24301 aacgaggcta aaattactga atacaatccg gcgaaatcgc gcgaacagtt gaaggcgttg
    24361 gggctggaaa acttaacgct gaaactgtgg gtgcccacac gttcgcaggc gtggaacccc
    24421 agtccactga aaactgccga actgattcag gcggatatgg cgcaggttgg cgtaaaagtg
    24481 gtgattgtgc cggtagaagg tcgctttcag gaggcgcggt tgatggatat gagccatgat
    24541 ctgacattat ccggttgggc gacggacagt aacgacccgg acagtttctt ccgtccttta
    24601 ctgagctgcg cggcaattca ttcacagacc aacctcgccc actggtgcga tccgaaattc
    24661 gacagcgtgt tgcgtaaggc gctctcctcg cagcagctgg cggcgcgtat tgaagcctat
    24721 gacgaagcgc agagtattct ggcgcaggaa ttacctattc tgccgctggc gtcgtcattg
    24781 cgtttgcagg cctatcggta cgatatcaaa ggtctggtac ttagcccgtt tggtaacgcc
    24841 tcctttgctg gggtgtatcg cgagaaacag gatgaggtga aaaaaccatg attatcttca
    24901 ccttacgccg cattttgtta ttgattgtca ccttgttcct gctgaccttc gttggcttca
    24961 gcttgagcta tttcacccct catgcgccgt tacaaggcgc gtcactgtgg aatgcctggg
    25021 tgttctggtt taacggcctg atccactggg attttggtgt ttccagcatt aacggtcaac
    25081 cgattgccga acagttaaaa gaggtcttcc cggcgacgat ggaactgtgc atcctcgcct
    25141 tcggttttgc gctgattgtt ggcatccccg tggggatgat tgccgggatt acgcgccata
    25201 agtggcagga taacttgatc aacgccatcg ccttactggg cttttcaatc ccggtgttct
    25261 ggctggcgct tctgttgacg ctgttttgtt cactcacgct aggttggttg cccgtttcag
    25321 ggcgtttcga tctgctctac gaagtgaaac cgattaccgg ttttgcgttg attgatgcct
    25381 ggctttcgga ctcaccatgg cgggatgaaa tgatcatgag cgcaatccgc catatgatat
    25441 tgcccgtgat caccctgtcg gttgcgccaa caactgaagt gatccgcctg atgcgtatca
    25501 gcactatcga agtgtatgac cagaactatg taaaagcggc ggcgacccgc ggtttgtcgc
    25561 gctttactat tttgcgtcgc cacgttctgc ataacgcgtt accgccggtt attcctcgcc
    25621 tgggcttaca gttttccacc atgctgacgc tggcgatgat caccgaaatg gtttttagct
    25681 ggccgggttt agggcgctgg ttaattaacg ccatccgcca gcaggattac gcagccattt
    25741 ccgccggagt gatggtgtgt ggctcactgg tcattattgt taacgtgatt tctgatattt
    25801 tgggtgccat ggctaaccct ctgaaacata aggaatggta tgccttacga tagcgtatac
    25861 agcgaaaagc gcccgccggg cacgctgcgt accgcctggc gcaaatttta tagtgacgcc
    25921 tctgcgatgg tcggcctgta cggctgcgcg gggctggctg tactgtgtat ttttggcggc
    25981 tggtttgcgc cttacggcat cgaccagcaa tttctcggtt atcaattatt gccgccatca
    26041 tggtcgcgct atggcgaagt ttctttcttc ctggggactg acgacctcgg acgcgatgtt
    26101 ttaagccgtt tactgagcgg agctgcgcca accgtgggtg gcgcatttgt ggtgacgctt
    26161 gccgcgacga tctgtggcct ggtactgggc accttcgccg gggcgacaca cggcctccgc
    26221 tcagcggtgc ttaaccatat tctggatacc ttactggcga tcccttcgct gctgttggca
    26281 attatcgttg tagcgtttgc cggaccgagt ttgtcccacg ctatgtttgc tgtctggctg
    26341 gcattgctgc cgcgtatggt acgttcgatt tacagcatgg tgcatgacga actggaaaaa
    26401 gagtacgtta tcgccgcccg tctggatggc gcatcaacgc tgaatattct ctggtttgct
    26461 gtgatgccaa acatcaccgc cgggctggtc accgagatca cccgcgcact gtcgatggca
    26521 attctcgata tcgccgcgct gggctttctc gatctcggcg cacaactccc ctcgcctgaa
    26581 tggggagcga tgctcggtga tgcgctggaa ctgatttatg tcgccccgtg gactgtcatg
    26641 ctgccaggtg cggcaattat gattagcgtg ttgttggtta acctccttgg cgatggcgtt
    26701 cgtcgtgcga ttattgcggg ggtggaataa tgccattact cgatattcgt aacctgacca
    26761 ttgaatttaa aaccggtgat gagtgggtta aagccgtcga ccgggtaagt atgaccttaa
    26821 ccgaaggtga aatccgcggt cttgttggtg aatccggttc cggcaagagt ttgattgcga
    26881 aagcgatttg tggggtgaat aaagataact ggcgtgttac tgctgaccgt atgcgttttg
    26941 atgatatcga tttgctgcgt ctctccgcac gcgaacggcg caaactggtt ggccataacg
    27001 tgtcgatgat tttccaggaa ccgcagtcgt gtcttgaccc ttcagaacgt gtgggtcgcc
    27061 agttgatgca aaacatccca gcctggacct ataaaggccg ttggtggcag cgttttggct
    27121 ggcgcaaacg ccgtgccatt gaactgctgc accgcgtggg gattaaagat cacaaagatg
    27181 cgatgcgcag ttttccctat gagttgaccg aaggtgaatg tcagaaagtg atgatagcca
    27241 ttgcactggc gaatcaaccg cgtctgctga ttgctgacga accgaccaac tcaatggagc
    27301 caacaaccca ggcgcaaatc tttcgcctgc tgacgcgtct caaccaaaac agcaatacca
    27361 ctattttgct tatcagccat gacttacaaa tgcttagcca atgggcggat aaaattaacg
    27421 tgctttactg cggtcaaaca gtggaaaccg cgccgagtaa ggagttggtg acgatgccac
    27481 atcatcctta tacccaggcg ctgatccgcg cgataccaga cttcggcagc gcgatgccgc
    27541 ataaaagtcg cctcaatacg ctgcccggcg ctatcccact gctggaacag ttaccgattg
    27601 gttgtcgtct ggggccacgt tgcccgtatg cacaacgaga atgcattgtg acgccacgtt
    27661 tgacgggggc gaaaaatcat ctctatgcct gtcatttccc gctgaacatg gagaaagagt
    27721 gagatgatcg aaacgctgct tgaagtgcgt aatttaagta aaacgttccg ctaccggacc
    27781 ggctggtttc gtcgtcagac cgtagaagcg gtaaaaccct tgagctttac gctacgtgaa
    27841 ggccagacac tggcgattat tggcgagaat ggttcgggta aatccacgct ggcaaaaatg
    27901 ctggcgggaa tgatagagcc taccagcggc gagttattga ttgacgatca tccactgcat
    27961 tttggcgatt attccttccg cagtcagcgt attcgcatga tttttcagga tccttcgacc
    28021 tcgttgaatc cccgtcaacg tatctcgcaa attctggatt ttccactgcg cctgaacacc
    28081 gacctggaac cggaacagcg tcgcaaacag attattgaaa cgatgcgtat ggttgggctg
    28141 ctaccggatc acgtcagtta ctatccgcat atgttggcac ccggacaaaa acagcgtctg
    28201 ggtctggcgc gcgcgctaat attgcgccca aaagtcatta ttgccgatga agccctcgct
    28261 tcactggata tgtcgatgcg ttcgcagttg attaatctga tgctggagtt acaggaaaaa
    28321 cagggcattt cgtatattta tgttacccag catatcggaa tgatgaagca cattagcgac
    28381 caggtgctgg tgatgcatca gggcgaggtt gtcgagcgag gcagcaccgc ggatgtgctg
    28441 gcatcgccgc tgcatgaact caccaaacgg ctgattgccg ggcactttgg cgaagcattg
    28501 acagcggatg cgtggcgtaa agatcgctaa ttttctgaat cctccttcca gattatccac
    28561 ctcattctga caagagatta aatcttgtca gaatgaggtg atggataaaa taaaatcaaa
    28621 tgcgcgtgat ttaagacgca atctcacttt gcaggaacga aagctctggc gatatcttcg
    28681 cagccgacgt tttggtgatt tcaaatttcg ccgtcaacat ccggtgggga gctacattct
    28741 cgattttgct tgctgctcgg cgcgcgtagt cgttgagctg gatggtgggc agcatgattt
    28801 ggcagttgcc tatgatacca ggcgcactag ctggcttgag tcgcagggct ggaccgtgct
    28861 gcgtttctgg aataacgaga ttgattgtaa tgaggaggcg gtgccggaga ttattctgca
    28921 ggaactgaac cgccgatcac cctcaccctg aaagggcgag ggggccgacc gagccgaata
    28981 gctgttgtgg tgaaaacatg gagacagtgc tggagaatat tcggcaaggt ctgaaccatc
    29041 ccagccatcg ccataaaagg gttaggggct gtatgagcct gtttgttgct ggggtaacaa
    29101 tatttgcaca atatggtccc ctcgcccctc tggggagagg gttagggtaa ggggaaaagc
    29161 gcccaccctg ccgcagcctg ctccggtcgg acctggcaac tatagctact cacagccagg
    29221 ttgattataa taaccgttta tctgttcgta ctgtttacta aaacgacgaa tcgcctgatt
    29281 ttcaggcaca acaagcatca acaataagga ttaaagctat gggttttctt tccggtaagc
    29341 gcattctggt aaccggtgtt gccagcaaac tatccatcgc ctacggtatc gctcaggcga
    29401 tgcaccgcga aggagctgaa ctggcattca cctaccagaa cgacaaactg aaaggccgcg
    29461 tagaagaatt tgccgctcaa ttgggttctg acatcgttct gcagtgcgat gttgcagaag
    29521 atgccagcat cgacaccatg ttcgctgaac tggggaaagt ttggccgaaa tttgacggtt
    29581 tcgtacactc tattggtttt gcacctggcg atcagctgga tggtgactat gttaacgccg
    29641 ttacccgtga aggcttcaaa attgcccacg acatcagctc ctacagcttc gttgcaatgg
    29701 caaaagcttg ccgctccatg ctgaatccgg gttctgccct gctgaccctt tcctaccttg
    29761 gcgctgagcg cgctatcccg aactacaacg ttatgggtct ggcaaaagcg tctctggaag
    29821 cgaacgtgcg ctatatggcg aacgcgatgg gtccggaagg tgtgcgtgtt aacgccatct
    29881 ctgctggtcc gatccgtact ctggcggctt ccggtatcaa agacttccgc aaaatgctgg
    29941 ctcattgcga agccgttacc ccgattcgcc gtaccgttac tattgaagat gtgggtaact
    30001 ccgcggcatt cctgtgctcc gatctctctg ccggtatctc cggtgaagtg gtccacgttg
    30061 acggcggttt cagcatcgct gcaatgaacg aactcgaatt gaaataatca ttctgttggt
    30121 aaatattggg cggcgttctg ccgcccgtta tctctgttat acctttctga tatttgttat
    30181 cgccgatccg tctttctccc cttcccgcct tgcgtcagga taacgatttc ctttacgacc
    30241 aaggagcgcc catggaacaa cgccacatca ccggcaaaag ccactggtat catgaaacgc
    30301 aatccagtac tgcggagtat gacgttctgc ctctggtccc ggaagccgca aaggtcagcg
    30361 atccctttct gctcgacgtg atccttgatg aagaaacgct ggctcccttc ctttcatggc
    30421 tggtccctgc gcgcgttctt gcagtggaat tgttccctga ccagcttacc gtgacccgtt
    30481 cacagacctt caccgcttat gaacgcttgt ctacggccct gacggttgct caggtttgcg
    30541 gcgtccagcg gttatgtaac tactattcgg cgcgacttac gccgctcccc gggcctgatt
    30601 cctccaggga aagcaatcat aggttggcac aaatcacgca atatgcccgc caactggcta
    30661 gctcgccttc tattatcgac aaccgatcgc gccagcatct gaatgacgtc ggtcttactg
    30721 cctgggactg tgtgatcatt aaccaaatca ttggttttat tggctttcag gcgcggacca
    30781 ttgcgacatt tcaggcttat ctcgggcacc cggtacgctg gttacccggt ctggaaatac
    30841 aaaactacgc cgacgcgtca ctgtttgctg atgaatcaat acgctggcga agcagctatg
    30901 aggtggaaaa actacctgaa gagtacacaa aaagttcaac agcagaactt tgccaactgg
    30961 ccgaaacact ctctctccac cctatttcac tttcccttct cgaaaggttg ttaaacagta
    31021 cacgggttaa tacacagccg gataataagc ttgcggcgtt gttatgcgcg cggataaatg
    31081 gcagtcctgc ttgttttgcc gcctgtatgg attcatcaaa tgaatataaa aaatcagccc
    31141 ccttctgcgc aagggcgaaa atgaaattaa ccaatgggct gaccgtcatt ctgttgagca
    31201 cgctaccgtt caggcgatac aatggctgac ccgagcaccc gatcgcttta gcgccgccca
    31261 gttcagccct ttactcggac acgaaaaatc atcaacgcag attattaatc tgctggtatg
    31321 gagcgggctg tgtggctgga taaatcgctt aaaaatcgcg ttgggtgaga catattaacc
    31381 ttgccgcgtc agacagattc gcgtaaaact gtcagccgct ctaatggcca ccaaaataga
    31441 caattatgtt tcaggacaac ccgctgctag cgcagcttaa acagcaactg cattcccaga
    31501 cgccacgcgc tgaaggggtg gtaaaagcca cagaaaaagg ctttggcttc ctggaagtcg
    31561 acgcgaaaaa aagttatttc attccgccgc cgcagatgaa aaaagtcatg catggcgacc
    31621 gaattatcgc ggtgatccac agtgaaaaag aacgtgaatc cgcagagcca gaagaactgg
    31681 ttgaaccgtt cctgactcgt ttcgtgggta aagttcaggg caaaaatgac cgtctggcca
    31741 tcgttcctga tcatccactc ttaaaagacg ccattccttg ccgcgcagcc cgtggcctga
    31801 accacgagtt taaagaaggc gactgggcgg ttgccgaaat gcgccgtcat ccgctgaaag
    31861 gcgatcgttc tttctatgct gaactgacac aatacatcac ttttggtgac gaccactttg
    31921 taccgtggtg ggttaccctt gcgcgccata atctggaaaa agaagcacca gacggcgtcg
    31981 ctaccgaaat gctcgatgaa ggtctggttc gtgaagatct gaccgcgctg gattttgtca
    32041 ccatcgacag tgccagcaca gaagatatgg atgacgccct tttcgctaag gcgttgccgg
    32101 atgacaaact tcagctgatt gtggcgattg ccgatccaac cgcgtggatt gctgaaggca
    32161 gcaagctgga caaagccgcg aaaattcgcg cattcaccaa ctatctgcct ggcttcaaca
    32221 tccctatgct tcctcgcgag ctttctgacg atctctgctc actgcgcgcc aatgaagtcc
    32281 gcccggtact ggcatgccgc atgacgctct ccgctgatgg caccattgaa gataatatcg
    32341 aattctttgc cgccaccatc gaatccaaag cgaagctggt gtatgaccag gtttctgact
    32401 ggctggaaaa taccggtgac tggcagcctg aaagtgaagc aattgccgaa caagtccgtt
    32461 tgctagcgca aatttgccaa cgccgcggcg agtggcgtca taaccacgca ctggtgttta
    32521 aagatcgccc ggattaccgc tttattctcg gtgaaaaagg tgaagtgctg gatatcgtcg
    32581 ccgagcctcg tcgcattgcc aaccgtatcg tcgaagaagc gatgattgcc gctaacattt
    32641 gtgcggcccg cgttctgcgc gataagctcg gttttggcat ctataacgtg catatgggct
    32701 ttgatccggc gaatgccgac gcgctggcag cgttgctgaa aacgcacggt ctgcatgtcg
    32761 atgccgaaga agtgctcacg ctggacggtt tctgcaaact gcgtcgtgaa ctggatgcgc
    32821 aaccaactgg tttcctcgac agccgcattc gtcgcttcca gtcatttgct gaaattagca
    32881 ctgaacccgg tcctcacttt ggcctcggtc tggaagcata cgccacctgg acttcgccga
    32941 tccgtaaata tggcgacatg atcaaccacc gtctgctgaa agcggttatc aaaggcgaaa
    33001 ctgcgacgcg tccgcaggat gaaatcactg tccaaatggc cgaacgtcgc cgtctcaacc
    33061 ggatggcaga acgtgatgtt ggcgactggt tatacgcacg cttcctgaaa gataaagccg
    33121 ggaccggcac ccgtttcgcg gcggaaattg tcgatatcag ccgtggcggc atgcgtgtgc
    33181 gtttggttga taacggcgct atcgccttta ttccggcacc tttcttacac gctgtgcgcg
    33241 atgaactggt ttgcagccag gaaaacggca ctgtacaaat taaaggtgaa acggtttaca
    33301 aagtaactga cgttattgac gtcaccattg ccgaagtccg catggaaacc cgcggcatta
    33361 ttgcgcgccc ggtcgcgtaa tctcctttca cggcccattc ctcatggatg ggccgtttat
    33421 ttccccgctc taccttcgtt atatccaggc agatttttta actgactttc gtttgaaaac
    33481 tggcgttttt ccaaaactgg attacaaata aatatataaa aaaacaatgc gtttagccgc
    33541 cgttaatttc atgtaagtca tgttgttcct tttatttaac cgcggactcc gcagttaacc
    33601 ggaggatatg catcatgaaa accgttaggg agtccacaac gttgtacaac tttctcggat
    33661 cgcacaatcc atactggcgg ttgacggaaa gcagcgatgt tttgcgcttt tttaccaccg
    33721 aaaccacaga acctgatcgt actttgcagt tatctgccga acaggctgct cgcatcaggg
    33781 aaatgacggt catcacctcc agcctgatga tgagtctgac cgtcgatgag agcgatcttt
    33841 ctgtgcatct ggtaggacga aaaatcaata aacgggaatg ggctggcaac gcgtctgcat
    33901 ggcatgacac accggcagtt gctcgtgatt tatcacacgg gctttccttt gctgagcagg
    33961 tagtttctga agcacattcc gcaatagtga ttctcgacag ccgggggaat atccaacgct
    34021 tcaatcggtt atgtgaagat tacactgggt tgaaagaaca cgacgtcatt ggccaaagcg
    34081 tgtttaaact gtttatgagc cgtcgtgaag ctgcggcatc caggcgcaat aaccgtgtat
    34141 tttttcgaag cggcaatgca tatgaagtcg aactgtggat accaacacgt aaaggccagc
    34201 ggctgtttct gtttcgcaat aaaataaatt tgtccacagc ggcagtggca aaaacgagat
    34261 ttttttaatc tgttccggta ccgacattac cgaagagcgc cgcgctcagg agcgactgcg
    34321 tattctggca aataccgaca gtatcaccgg actgccgaat cgtaacgcaa tgcaggagtt
    34381 aatcgatcac gctattaatc aggctgataa caataaagtt ggggttgtgt atcttgattt
    34441 ggataatttt aaaaaggtca acgacgccta tgggcatttg tttggtgacc agttattacg
    34501 cgacgtgtca ttggctattt taagctgtct cgaacatgac caggtgttgg cgcgtccagg
    34561 tggcgatgag tttctggtac tggcatccaa tacctcacaa agcgcgctgg aagcaatggc
    34621 atcacgaatt ttgacccgct tacgcctccc ctttcgtatt ggtttaattg aagtttatac
    34681 cagctgttca gtaggtattt cgctctctcc cgaacatggt tcggacagcg cgactattat
    34741 tcgtcacgcc gacacagcaa tgtacacagc gaaggaaggc ggacgaggac aattttgcgt
    34801 ttttactcca gaaatgaatc agcgggtatt tgaatatctc tggctggata ccaacttgcg
    34861 taaagcactg gaaaacgatc agttggttat tcactatcaa ccgaaaatca cctggcgtgg
    34921 cgaagtgcgc agtctggaag cactagtacg ttggcagtca cctgaacgtg ggttgattcc
    34981 accgttggac ttcatttcct acgccgaaga gtcagggcta attgtgcctt taggccgttg
    35041 ggtgattctc gatgtcgtac gccaggtggc aaagtggcgg gataaaggca taaacctgcg
    35101 agtggcggta aatatttctg cacgtcagct cgccgatcaa accattttca ccgccctgaa
    35161 acaggttctc caggaactca attttgaata ctgccctata gatgttgaac tgacagagag
    35221 ttgtctgatt gagaatgatg aactggcact gtctgttatt caacaattta gccaactagg
    35281 tgcgcaagtg catctggatg attttggtac cggctactct tcactttcgc aactggcgcg
    35341 ctttccgatc gatgccatca aacttgacca ggtttttgtt cgagatattc acaaacaacc
    35401 tgtctcgcag tcactggtcc gggcgatcgt cgctgtggcc caggcattga atcttcaggt
    35461 gatcgccgaa ggtgtagaga gtgcaaagga agatgctttt ttaaccaaga acgggatcaa
    35521 tgagcggcaa ggatttttgt ttgccaaacc gatgcccgcc gtcgccttcg aacgctggta
    35581 taaacgctat ctgaagcgcg cataagtaga tgaatatata tacttagcgc tatatcccgt
    35641 cattatatat cgtattcctg gatgataatt agccgttgct gttaaacact tctcttttag
    35701 cggcaatttt tgcattattc attttccact tttgctataa ggatgtcctt gatgtctgaa
    35761 tttgatgccc agcgcgttgc cgaacgtatt gatattgtgt tagatatcct tgtggcaggc
    35821 gattaccact ctgcgatcca taatctcgag atacttaaag cagaactctt gcgccaggtt
    35881 gctgaatcta cgccagacat cccgaaggca ccgtgggaga tttaacaatc gtgccttcag
    35941 gttttcctat ttgaggatgc ccggcgcgtc agtatttttt tatttagtat tataacgtta
    36001 taagaattac agcgatgaat tcccgacaac aaactattct acagatggtc attgaccagg
    36061 gtcaggttag cgtaaccgat ctggcaaaag ccactggagt ttctgaagtt accattcgcc
    36121 aggatctcaa caccctcgaa aaactgagtt acctccgccg tgcacatggc tttgcagttt
    36181 cgcttgatag tgatgacgtc gaaacccgta tgatgagcaa ctatacgctg aagcgtgaac
    36241 tcgccgagtt tgccgcgtca ctggttcaac cgggcgaaac catctttatc gaaaatggca
    36301 gcagcaatgc cctgctcgcc cggactctgg gggagcagaa gaaaaatgtc actatcatca
    36361 cggtcagcag ctacatcgcg catttgctga aagacgcgcc ttgtgaagtc attttgctcg
    36421 gtggcgtgta ccagaaaaaa agcgaaagta tggttggccc gttgacacgc cagtgcatcc
    36481 aacaggtgca tttcagcaaa gcatttattg gtattgatgg ctggcaacct gaaactggat
    36541 tcaccggtcg cgatatgatg cgtaccgatg tggtcaatgc cgtgctggaa aaagagtgcg
    36601 aagcgatagt cctgactgac agctcgaaat ttggtgctgt acattcatac tccatcggtc
    36661 ccgttgagcg attcaatcgc gtgattaccg attcgaaaat acgcgccagc gatctgatgc
    36721 atcttgagca cagtaaactc accgttcacg tcgttgacat ttaaccattt cccttctcta
    36781 cggatgattt gcagtttggc aaatcatccg ctctaagatg attcctggtt gataattaag
    36841 actatttacc tgttattaac acgcttaaga tataaaatta ttatcagcga tataacagga
    36901 agtcattatc acctgcgtga tataaccctg cgcgcgagca gatttcacgg aataatttca
    36961 ccaggcttat tcttagctat tatagttata gagagcttac ttccgtgaat cataaattca
    37021 ggagagagta ttatgtttgt aacgagcaaa aaaatgaccg cggctgttct ggcaattact
    37081 ttggcaatgt ctctgagtgc ctgttctaac tggtctaaac gggaccgcaa caccgcaatc
    37141 ggcgcgggtg caggggcatt aggcggtgca gtactgaccg atggcagtac gttgggtaca
    37201 ttaggtggtg cagctgtcgg tggtgttatt ggtcaccagg tcggtaaata atcgtaaata
    37261 aatgtcgatt atcgactcgc aattataata cgcttgattc tgttcagtac gaaataactg
    37321 aataatacgc acagtaaaat attcgaaagc cacggatata tccgtggctt tttatgttaa
    37381 ccgcctgcga gttttacctt catccctttc gcttcaagta gtgattttaa taaatcacgc
    37441 ttatcgccct ggatttcaat aactccatct ttaactgctc cgccgcagcc gcattttttc
    37501 ttcagttccg ctgcaagttt tgtcagttcg gcatcatcga gatcgacacc ggtaatcagg
    37561 caaacgccct tacctttacg tccactggtc tgacgctgaa tacgcaccac accgtcgcct
    37621 ttagggcgta caggggccgc tttgggctca tcaatacgtc cggtttccgt tgagtagacc
    37681 agacggctgt tggaatcact catcatgcac tccgctgtaa agaggcgttg atcgctttca
    37741 gcgtctgcgc tggatctacc gattgcgtta ccgggcgacc aatcaccata taatcaacac
    37801 cagccgacaa cgcctgttct ggcgtcataa tgcggcgctg gtcaccagct tcactcccct
    37861 gcggacgaat gcccggcgta accagtttga actcctgacc gaatacctgt ttaaagcgca
    37921 cagcttcctg agcagaacac accacaccat caaggccaca tttttgcgtc agtgccgcca
    37981 gacgttctgc ataatctgca ggtgacagtg tcatgccaag atcgaccagg tcgctggctt
    38041 ccatgctggt caacactgtc acagcaatca aaagcggtgc atctttgcca aacggaacca
    38101 gtgcctcacg cgctgcggtc atcatacgcg ccccaccaga ggcatgaaca ttcaccatcc
    38161 acacgcctaa gtcagctgca gcagcgacag cgtgcgctgc agtgttagga atatcgtgga
    38221 atttcaggtc aagaaagata tcaaaaccac gctgttgaag ttcgcgcaca aactgtggcc
    38281 caaacaatgt aaacatctct ttgccgacct tcagacgaca atcgcgtggg tcgatcttgt
    38341 cgacaaagga cagcgcgtca tcacgattat gataatcaag ggcaacaacc acaggagaat
    38401 tcgtaacagc gcgggaagaa gatgaagcag ttaacgtcat gaccagacct tcttgatgat
    38461 gggcaccgga agtggcgcga aacaggtaaa cggcgagcat tctacccgtg gaaataaaaa
    38521 attaacagtt gcgatttttc ccctggcagg ctttttctgc tccgtcgttg ccggagcaca
    38581 atggaacatc aataactagt atgttgtaac taaagtcgga tttttttaaa aattacaggc
    38641 catcaagacc gcgaatcggt ttaatggttg accaggcccg acaagacgga caatgccagt
    38701 agagggtgta tgcggtaaaa ccacatttct ggcagcgata acgaggctta ctacgtacct
    38761 tttcgccaac catgtcacgc agcaccatca ggctctcttt ggcacgccct tcttccgctt
    38821 catttaagtg gtaatccatt aacttatgga acacacgcat ggtcggatga cgctgaagct
    38881 ggcgcgtaat atagacctgt gcggcctcac taccgtcgcg cgcttcgatg atatcagcaa
    38941 gcatcaattc agcatcggca ccggtgttct cttccaccgc gcgttgcagg aattctgccc
    39001 attcggcagt tttacccaac tgctgatagc aggtttgcaa catttccagc gtttcgctga
    39061 ccagttctct gtcctgggat atcacgcgtt gcaaactttc gacggctttg gcgtattctc
    39121 ctttcgccat aaacacgcgt cccatcatga tggatacgcg ggcgctgttt ttatctgccg
    39181 ccgccccttt ttttagcaag gtcatggcac gatcgagatc gtcgctggcc atatgctgca
    39241 gggctaactc acagtagaaa tgggcaattt cgacgcgctg tttatcttta cccagcttca
    39301 ccaggcgttc ggcaacatca attgctttct gccactcgct ggtagcctgg tagatttgta
    39361 gcaactgttg cagcgcgcca atgcggaagt cagtttcatc ggtcagctga ttgaacatgt
    39421 cttccgcgcg gtcgtataac ccggcggcca tgtaatcacg ccccagttgt tgaatcgcca
    39481 acagacgctg ttcataggtc agtgaggcgc tttccattag ggtctgatgg atgcgaatag
    39541 cgcgatcaac ttcgccacgc gaacggaaca ggtttccgag cgtaaggtgg gcttcaacgg
    39601 tgcctgtatc ctctttaagc atatcgagaa acaggtctac cgctttatcc tgttgattac
    39661 taagcaggaa gttaaccccc gctacgtaat cacgcgacaa gcggttggct tcatcttgct
    39721 tgttttgttg cgcacttctg cggcccatat accagccata ggcagcggct acaggcaaaa
    39781 gcagaaacaa caactccagc atagaaagtt attccttcgc cgctgacgag tgcggcacta
    39841 cagccacgtc agtcgcgggt gaaagctggt tttccagtcg ctttatttta cgttcagcgc
    39901 gcgccaggga aacacgaact cgcagccaga acaggccaca aatcaaccaa ccgatagcaa
    39961 cccccgcagc aaacaatacc gccagcaatg tggaaatacg gtactcccct tgcgctaaca
    40021 gataattaaa cgtcacctgt tgatcgttct gcgcacccaa cgtgaccgaa atcacgaaga
    40081 tcgctaacac cagtaagaaa atgagtaaat atttcacatt acttcccgtt atgtggttaa
    40141 agcgaataaa ttatggtcaa cttaccgcaa tagcccttta aagtaccatt ttcgcgccgt
    40201 ctgcgaaatg gcatccacgc gcattgttga tgatttaagg gctaaattcg gaaaatcaac
    40261 cagcgttaac tttcttgttc tcgttgcgct atttcgcgat tttcttccgc aggtggtgtt
    40321 aatggcccac aaattcgttg cgcaagccac gttgccaccg ccaccagcgc ccacgaaatc
    40381 aacgtagcta ctaccagatc gcgtggccaa tgcatcccga gcagcaggcg gcttcccatg
    40441 actcccgttg cccagaccag caagatagca atggttaacg ttcgccgacg cggccacagc
    40501 aaaccaacgg ccagcagtgc ccaactggca gcaaacatcg tgtgaccggc aatggttaac
    40561 gttcgccgac gcggccacag caaaccaacg gccagcagtg cccaactggc agcaaacatc
    40621 gtgtgaccgg aaggaaaggc aaaccccgtc tctttctgcc agtgtgaacg caaatattgt
    40681 gggatatttt tctcttcagc caactgttct ttcactagat ttccgcgttc tgctcgcttt
    40741 aaagtgtaga actcatcaac cggaatatga tgtgtttttt ccagccagat aacaaaaggt
    40801 cgtggttcct ggactttgtc tttgatccag gatttaacgc cttgtcccac aatgattacg
    40861 gccgccagaa tggcaaataa tacaaaggca gccttaatgc aaaaacgcag acaccagaga
    40921 aaccagccga ataaaatcaa atgtgtaatg acgccccagg gctgggtgac agtttcagta
    40981 acccaaaaag ccgcttttag tagccaactt tgttctccag gttgccaacg ccagccagaa
    41041 atccatacgg ctactggcat gacaagcaat agtgcagctc ccactgcggt acgtctggca
    41101 atcgaacgca tggcctctcc tttttgataa gtcccacaat cataactgaa aacgccagtt
    41161 ccaggaaaaa ttgacagatt tgtgccattc cgtgaacgat cgacgcgtcg tgattaggtg
    41221 aaccccttct cgttatggca aaataagcca atacagaacc agcattatct ggagaatttc
    41281 atgcagctta aacgtgtggc agaagccaaa ctgccaaccc catggggcga tttcctgatg
    41341 gtgggatttg aagaactggc aaccggacac gatcatgtcg cgctagtcta tggcgatatt
    41401 tccgggcata ccccggtact tgcgcgcgtc cattccgaat gtctgaccgg tgacgccctg
    41461 ttcagcttgc gctgcgattg tggcttccag ctcgaagcgg cattgacgca aattgccgag
    41521 gaaggccgtg gtattttgct gtatcaccgt caggaaggtc gtaacattgg tctgctgaat
    41581 aaaatccgcg cttacgcact gcaggatcaa ggttacgata ccgtagaggc taaccatcag
    41641 ttaggcttcg ccgctgatga gcgcgacttc actctttgcg ctgatatgtt caaactcctt
    41701 ggcgtcaatg aagtccgctt gttaaccaat aacccgaaaa aagtcgaaat tctgaccgaa
    41761 gcagggatta atattgttga acgcgtacca ttgattgtag gtcgtaaccc caataacgaa
    41821 cattatctcg ataccaaagc cgagaaaatg ggtcatttgc tgaacaaata accctctcgc
    41881 attgtggaat taatttgctt gccggaagca aaataaccgg caagcaaata tttgttactt
    41941 cagcatatta cgaatgacat aatgcaaaat gccgtcgttc tggtagtagg tcaactccgt
    42001 cgcggtgtcg atacgacaac ggcagggtac gacctcctgg ctaccatccg cgcgcgtaag
    42061 cgtcaccgga accgtcgcac cgggttgtag attttgcaga tcgccaatat caatcttctc
    42121 ttccccggtt agtcctaacg ttttacgcgt tacgccttgc ggaaattcca gcggcaggat
    42181 gcccatgcca attaaattcg aacggtgaat tcgttcaaac gattcggcaa tcaccacacg
    42241 aattccaagc agacgaggac cttttgccgc ccagtcacgg cttgagcctg aaccatactc
    42301 tttcccggca atcaccgcca gcggcgtttg ttcctgctta tagcgcatcg cagcatcata
    42361 aatagagact acgtcgctgt caggtaaatg ccgcgtcatc cccccttcaa cgccaggcac
    42421 catttcatta cggatgcgaa tattggcgaa ggtgccgcgc atcatcactt catggttacc
    42481 acgccgcgaa ccgtaggagt taaagtcttt tcgctcaaca ccccgacctt gtagatatcg
    42541 acccgctggg ctgtcgggct taatactgcc cgccggagag atatggtcag tggtgactga
    42601 atcccccagc attgcgagga tccgcgcacc gtgaatatct tccactggtg ctggtgttgc
    42661 ctgcatttca tcaaagaaag gcgataagcg aatataggtt gagtcctcct gccaaccgta
    42721 ggtatcggat cgtgtgacgt taatttcctt ccactctgct gtgccttcaa aaacttctgc
    42781 gtactctttg cggaacattt ctgtggagac ttgttctacc gcacgggcaa tttcttgtgc
    42841 cgatggccag atatctttca gataaaccgg atcgccttta cgatcatgac caataggctc
    42901 agaagccagg ttgatattca tatttcccgc cagcgcatag gcaaccacca gcggcggcga
    42961 ggccagccag ttggttttaa ccagcggatg gatacggcct tcaaagttac ggttgccgga
    43021 cagcactgca ccgacggtta aatcgccttt tttgattgcc gtttcgatag gatcgggcag
    43081 cggcccagag ttaccaatac aggtagtaca accgtatccc acaaggttaa accccagttc
    43141 gtcgagaaac ggtgtcagtt tcgcttttgc cagatagtca gaaacaactt tcgagcctgg
    43201 tgccagcgac gctttgaccc acggttgccg cttgaggcca agaattacgg cttttttcgc
    43261 cagcaagcct gcagccatca gcacacttgg gttagaggtg ttggtgcacg aggttatcgc
    43321 agcaatgacc acagcgccat caggtaactg atactgatgt ccgttcataa cataatcgac
    43381 cggctggcga tctttatgcg tggcattcac ttccagttcg ttactggcgg caaatacttt
    43441 tggtacatcg ggcagtgcaa cgcgatcctg tgggcgtttt ggccctgcca ggctcgcttc
    43501 aacgtcattc atatccagtt ctaacgtact ggtaaaaatc ggttcatcgc ccgggttacg
    43561 ccacatgccc tgcgctttgg catatttttc gaccaactca acctgatctt cgctgcgccc
    43621 gcttaaacgc atgtaatcga gggttacagc atcgattggg aagaagccac aggtggcacc
    43681 atattctggc gacatattgg caatggtggc gcgatccgcc agcggtagtg aatccagacc
    43741 atcaccataa aattcgacga atttctccac cacgccatgt ttgcgcagca tttgggtaac
    43801 agtgagaacc aggtctgtgg cggtaatacc ttcacgtaat ttaccggtaa gtttaaagcc
    43861 cactacgtcc gggataagca tggaaaccgg ctggcctaac attgcggctt ctgcttcgat
    43921 cccacccacg ccccacccca gcacgccaag gccgttgatc atagtggtgt gcgagtcagt
    43981 accaacgagt gtatccggat aagcaatcca ttcaccgtcc tgcaattcac tccacactgc
    44041 tttgccgaga tattcgaggt taacctgatg gcaaatgcct gtgcctggcg gcacgacgct
    44101 aaaccgactg aacgcttgct ttccccattt caggaacaca taacgttcgt ggttgcgctc
    44161 catttccagg cgtacgtttt cttcaaatgc ctcatcatca ccaaaacgat cgacggtcac
    44221 cgagtggtca atgaccaggt cgaccggtga gagcgggtta acctttgcag tatcgccgcc
    44281 gaggcgttta accgcttcgc gcattgccgc cagatcaaca acggcaggta cgccggtaaa
    44341 gtcctgcatc aacacccttg ccgggcggta ggcaatttca cggtcagcat gggcattttt
    44401 cagccatcct gccagcgcgt ggatatcctc ttcggtaacc gagttaccat cctgccagcg
    44461 cagcaggttt tcgagcaaaa ctttgagtga cttgggtaga cgggtgatat cgcccagtga
    44521 tttagcagca agcggcaggc tgtagtagtg gtaagtttta tctttggcct gcaacgtgtc
    44581 cttactggct tctcgtaggg ttgacgacat agctcctcct taatgacagg gttgcgaagc
    44641 cctgattctc ttcagggtga atattaaaga taacacaaac atcgcgtaac gatttgataa
    44701 caacccaaat tgataaaaga ggagaaacct tgtggcaaag caaatgacaa ccccgccgca
    44761 gcggggtcaa ggatcagtga gtaatgatcc agaggagttc ggcccagaag agcatcgaca
    44821 gagaaaacgc gcccatccag gaccaatatt tcatatttgt gttgatcata ggctacaccc
    44881 ttaacggtta ttattttgtg ttgagataac tcaagctgtt attgctgcgg tgtattaaaa
    44941 gccctttaat tcattaaaaa tgggtaataa gaatgatgat aattattgtt tcggctgatc
    45001 atcttcagca aacagttcta taaaggcttg ctgctgcgat gttagttgcc agtcgtctgg
    45061 aataacgacg gaacattccc gtttcgtttc tgtttcattg tcactatgct ggcataatgg
    45121 tgacgactcc agttgctcct gaccgaccat agttatcccc acactttcca aatcagcagt
    45181 gcgacaacca cccagaaaag tgcagaacca agaaaaaccg ccagccaggc tttacgtttt
    45241 aattcaggat cgcggcgggg ttcctgattt ccagaaggca ttgctaacct catataatcg
    45301 ccatcactta tcagcaagac gttgaacgat tggtgtaatt aatcatacga tgagacaaat
    45361 cctaaagaaa ctttagtcaa aa
//
LOCUS       DAJVQG010000012        43478 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJVQG010000012 DAJVQG010000000
VERSION     DAJVQG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43478
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.012"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(1..77)
                     /locus_tag="OQM83_000646"
     tRNA            complement(1..77)
                     /locus_tag="OQM83_000646"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Arg,seq:acg)
     gene            complement(81..173)
                     /locus_tag="OQM83_000647"
     tRNA            complement(81..173)
                     /locus_tag="OQM83_000647"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(137..139),aa:Ser,seq:gct)
     gene            complement(489..674)
                     /gene="csrA"
                     /locus_tag="OQM83_000648"
     CDS             complement(489..674)
                     /gene="csrA"
                     /locus_tag="OQM83_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCS1518991.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(909..3539)
                     /gene="alaS"
                     /locus_tag="OQM83_000649"
     CDS             complement(909..3539)
                     /gene="alaS"
                     /locus_tag="OQM83_000649"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCS1518992.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3667..4167)
                     /gene="recX"
                     /locus_tag="OQM83_000650"
     CDS             complement(3667..4167)
                     /gene="recX"
                     /locus_tag="OQM83_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCS1518993.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WLNFAD"
     gene            complement(4395..5456)
                     /gene="recA"
                     /locus_tag="OQM83_000651"
     CDS             complement(4395..5456)
                     /gene="recA"
                     /locus_tag="OQM83_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCS1518994.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5536..6033)
                     /gene="pncC"
                     /locus_tag="OQM83_000652"
     CDS             complement(5536..6033)
                     /gene="pncC"
                     /locus_tag="OQM83_000652"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCS1518995.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6178..7263)
                     /gene="mltB"
                     /locus_tag="OQM83_000653"
     CDS             complement(6178..7263)
                     /gene="mltB"
                     /locus_tag="OQM83_000653"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCS1518996.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7519..8082
                     /gene="srlA"
                     /locus_tag="OQM83_000654"
                     /gene_synonym="gutA"
     CDS             7519..8082
                     /gene="srlA"
                     /locus_tag="OQM83_000654"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCS1518997.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8079..9038
                     /gene="srlE"
                     /locus_tag="OQM83_000655"
                     /gene_synonym="gutE"
     CDS             8079..9038
                     /gene="srlE"
                     /locus_tag="OQM83_000655"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCS1518998.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            9049..9420
                     /gene="srlB"
                     /locus_tag="OQM83_000656"
                     /gene_synonym="gutB"
     CDS             9049..9420
                     /gene="srlB"
                     /locus_tag="OQM83_000656"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCS1518999.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9424..10192
                     /gene="srlD"
                     /locus_tag="OQM83_000657"
                     /pseudo
     CDS             9424..10192
                     /gene="srlD"
                     /locus_tag="OQM83_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            10298..10657
                     /gene="gutM"
                     /locus_tag="OQM83_000658"
     CDS             10298..10657
                     /gene="gutM"
                     /locus_tag="OQM83_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCS1519000.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10724..11497
                     /gene="srlR"
                     /locus_tag="OQM83_000659"
                     /gene_synonym="gutR"
     CDS             10724..11497
                     /gene="srlR"
                     /locus_tag="OQM83_000659"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCS1519001.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11490..12455
                     /gene="gutQ"
                     /locus_tag="OQM83_000660"
     CDS             11490..12455
                     /gene="gutQ"
                     /locus_tag="OQM83_000660"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCS1519002.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12452..13966)
                     /gene="norR"
                     /locus_tag="OQM83_000661"
     CDS             complement(12452..13966)
                     /gene="norR"
                     /locus_tag="OQM83_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCS1519003.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            14153..15592
                     /gene="norV"
                     /locus_tag="OQM83_000662"
     CDS             14153..15592
                     /gene="norV"
                     /locus_tag="OQM83_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCS1519004.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15589..16722
                     /gene="norW"
                     /locus_tag="OQM83_000663"
     CDS             15589..16722
                     /gene="norW"
                     /locus_tag="OQM83_000663"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCS1519005.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(16850..19102)
                     /gene="hypF"
                     /locus_tag="OQM83_000664"
     CDS             complement(16850..19102)
                     /gene="hypF"
                     /locus_tag="OQM83_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCS1519006.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19255..19782)
                     /gene="hydN"
                     /locus_tag="OQM83_000665"
     CDS             complement(19255..19782)
                     /gene="hydN"
                     /locus_tag="OQM83_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCS1519007.1"
                     /translation="MNRFIIADASKCIGCCTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19930..20940)
                     /gene="ascG"
                     /locus_tag="OQM83_000666"
     CDS             complement(19930..20940)
                     /gene="ascG"
                     /locus_tag="OQM83_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCS1519008.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21200..22657
                     /gene="ascF"
                     /locus_tag="OQM83_000667"
     CDS             21200..22657
                     /gene="ascF"
                     /locus_tag="OQM83_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCS1519009.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            22666..24090
                     /gene="ascB"
                     /locus_tag="OQM83_000668"
     CDS             22666..24090
                     /gene="ascB"
                     /locus_tag="OQM83_000668"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCS1519010.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24204..24623)
                     /locus_tag="OQM83_000669"
     CDS             complement(24204..24623)
                     /locus_tag="OQM83_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1519011.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(24620..24931)
                     /locus_tag="OQM83_000670"
     CDS             complement(24620..24931)
                     /locus_tag="OQM83_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HCS1519012.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25093..25866)
                     /locus_tag="OQM83_000671"
     CDS             complement(25093..25866)
                     /locus_tag="OQM83_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1519013.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            complement(25891..26361)
                     /gene="hycI"
                     /locus_tag="OQM83_000672"
     CDS             complement(25891..26361)
                     /gene="hycI"
                     /locus_tag="OQM83_000672"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCS1519014.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(26354..26764)
                     /gene="hycH"
                     /locus_tag="OQM83_000673"
     CDS             complement(26354..26764)
                     /gene="hycH"
                     /locus_tag="OQM83_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCS1519015.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(26761..27528)
                     /gene="hycG"
                     /locus_tag="OQM83_000674"
     CDS             complement(26761..27528)
                     /gene="hycG"
                     /locus_tag="OQM83_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCS1519016.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNRGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(27528..28070)
                     /gene="hycF"
                     /locus_tag="OQM83_000675"
     CDS             complement(27528..28070)
                     /gene="hycF"
                     /locus_tag="OQM83_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCS1519017.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(28080..29777)
                     /gene="hycE"
                     /locus_tag="OQM83_000676"
     CDS             complement(28080..29777)
                     /gene="hycE"
                     /locus_tag="OQM83_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCS1519018.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            complement(29795..30718)
                     /gene="hycD"
                     /locus_tag="OQM83_000677"
     CDS             complement(29795..30718)
                     /gene="hycD"
                     /locus_tag="OQM83_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCS1519019.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            complement(30721..32547)
                     /gene="hycC"
                     /locus_tag="OQM83_000678"
     CDS             complement(30721..32547)
                     /gene="hycC"
                     /locus_tag="OQM83_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCS1519020.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKFGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(32544..33155)
                     /gene="hycB"
                     /locus_tag="OQM83_000679"
     CDS             complement(32544..33155)
                     /gene="hycB"
                     /locus_tag="OQM83_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCS1519021.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(33280..33741)
                     /gene="hycA"
                     /locus_tag="OQM83_000680"
     CDS             complement(33280..33741)
                     /gene="hycA"
                     /locus_tag="OQM83_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCS1519022.1"
                     /translation="MTIWEISEKADYIAQRHCRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            33953..34303
                     /gene="hypA"
                     /locus_tag="OQM83_000681"
     CDS             33953..34303
                     /gene="hypA"
                     /locus_tag="OQM83_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCS1519023.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            34307..35179
                     /gene="hypB"
                     /locus_tag="OQM83_000682"
     CDS             34307..35179
                     /gene="hypB"
                     /locus_tag="OQM83_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCS1519024.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            35170..35442
                     /gene="hypC"
                     /locus_tag="OQM83_000683"
     CDS             35170..35442
                     /gene="hypC"
                     /locus_tag="OQM83_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCS1519025.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            35442..36563
                     /gene="hypD"
                     /locus_tag="OQM83_000684"
     CDS             35442..36563
                     /gene="hypD"
                     /locus_tag="OQM83_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCS1519026.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            36560..37570
                     /gene="hypE"
                     /locus_tag="OQM83_000685"
     CDS             36560..37570
                     /gene="hypE"
                     /locus_tag="OQM83_000685"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCS1519027.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            37644..39721
                     /gene="flhA"
                     /locus_tag="OQM83_000686"
                     /pseudo
     CDS             37644..39721
                     /gene="flhA"
                     /locus_tag="OQM83_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(39758..40111)
                     /locus_tag="OQM83_000687"
     CDS             complement(39758..40111)
                     /locus_tag="OQM83_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCS1519028.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(40188..40322)
                     /locus_tag="OQM83_000688"
     CDS             complement(40188..40322)
                     /locus_tag="OQM83_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1519029.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            40398..42959
                     /gene="mutS"
                     /locus_tag="OQM83_000689"
     CDS             40398..42959
                     /gene="mutS"
                     /locus_tag="OQM83_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCS1519030.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            43065..43478
                     /locus_tag="OQM83_000690"
                     /pseudo
     CDS             43065..43478
                     /locus_tag="OQM83_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
BASE COUNT        10293 a        11936 c        11326 g         9923 t
ORIGIN      
        1 tggtgcatcc gggaggattc gaacctccga ccgctcggtt cgtagccgag tactctatcc
       61 agctgagcta cggatgcaaa tggcggtgag gcggggattc gaaccccgga tgcagctttt
      121 gaccgcatac tcccttagca ggggagcgcc ttcagcctct cggccacctc accacacgcc
      181 tcttacgagt gcttcgaaga acttgtttat cgctcatcgt cgctgcgtgg cgcacatatt
      241 actttctgag acttataagt caaacaattt ttcccacact tttatcgttt gcacacttca
      301 cgctcaatta gtctgtaaaa acgtcaaaaa gagtgtttta tctacagaaa aacggaggtc
      361 tggcagatag cagtaatgca aaaaaatgga gacttaagtt gaatgaacgg gagtaaagcg
      421 aaaagactat agagtgaagg agaaattttg agggtgcgtc tcaccgataa agatgagacg
      481 cggaaagatt agtaactgga ctgctgggat ttttcagcct ggatacgctg gtagatctct
      541 tcacggtgaa cagaaacttc cttcggggca tttacgccaa tacgtacctg gttgcccttt
      601 acccctaaaa ctgtcacggt gacctcatcc ccaatcatga gggtctcacc aactcgacga
      661 gtcagaatca gcattctttg ctccttgaaa gattaaaaga gtcgggtctc tctgtatccc
      721 ggcattatcc atcatataac gccaaaaagt aagcaatgac aaacacatta catgtaagca
      781 gtcatggcat tacattctgt taaacctaag tttagccgat atacacaact tcaacctgac
      841 tttatcgttg tcgatagcgt taatgcgaat gccgtgaagc gagtccacgg cattgcctga
      901 cgcttatatt attgcaattt cgcgctgacc cagcctttca cactggctaa cgctgcaggt
      961 aaggccgcag catccgtacc accggcttgc gccatgtcag gacgtccacc acccttgccg
     1021 cccacctgct gagcgaccat accaatcagt tcccctgctt tcacacgatc tgtgacgtcc
     1081 ttagatacgc ctgcaatcag agaaacctta ccttcggcta ccgttgccag cacgataatt
     1141 gtcgacccca gctgattttt taaatcgtca accatggtac gcaacatttt cggctcaaca
     1201 ccgctaagct cgctaaccaa cagcttaaca ccattaacat caattgcctt actggaaaga
     1261 tttgcgctct cctgtgcggc agcttgttct ttaagctgtt gtagttcttt ttctaactga
     1321 cgcgtacgtt ccagtactga gcgcacttta tccgccagat tattgctatc gcctttcagc
     1381 agatgcgcga cttcgcttaa gcgatcgctg tctgcatgaa cggtggtgat agcgccttct
     1441 ccggttaccg cttcgatacg acgaacgcct gcagcagtac ccgattcaga gatgatgcgg
     1501 aacagaccaa tatcaccagt gcggctggcg tgagtaccgc cacacagctc ggtggagaaa
     1561 tcgcccatgc tcagcacgcg tacacgctca tcatacttct cgccgaacag cgccatcgca
     1621 cctttcgctt tcgccgcttc gagatccatg atgttggttt cgatcggcaa attgcgacga
     1681 atctgtgcgt tcaccaggtc ttcgaccgca cgaatctctt ccggtttcat cgcttcgttg
     1741 tgtgagaagt cgaagcgcag caccttgtcg ttaaccagtg aacctttctg cgatacatga
     1801 gtccccagaa cctggcgcag cgcagcgtgc atcaggtgcg ttgcggagtg attcagacga
     1861 atacgggcgc gacgagcctc atcaacatca gcctgcacag cgtcgcccac tttcagagaa
     1921 cccgcagcaa gtttaccgat gtgaccaatc gcctggccgt atttctgcgt atcttccacc
     1981 gcgaaggaga agttagcgcc tttcagttcg cctttatcac caacctgacc gccggattcc
     2041 gcatagaatg gcgtttgatc cagcacgacc acagcttcct ggcctgcatt gatggcatca
     2101 accgctttac catcaacaaa cagcgcggtc actttgccgt tcagttccag atggtcatag
     2161 cctttaaatt cagatgcact atcaacacgg atcattgcgt tgtaatcggc accaaagccg
     2221 ctggcttcgc gcgcgcgacg acgctgctct tccattgctg cttcaaaacc agcttcgtca
     2281 actttgatgt tgcgctcacg acaaacatca gccgtcaggt caaccgggaa gccataggtg
     2341 tcgtacagac ggaaagccgt ttcaccatcc agcgtatcac cagaaagttt tgccagctct
     2401 tcatccagca acgccagacc gcgctccaga gtacgggcaa actgctcttc ttcagtcttc
     2461 agcacctgct caacctgcgc ctgctggcgt ttcaggtctt cacccgcaga gcccataacg
     2521 tcgatcagcg gaccaaccag tttgtagaag aaggtttctt tcgcgccgag catattgccg
     2581 tgacgcactg cgcgacgaat gatacgacgc agtacataac cacggttttc attggacggc
     2641 attacgccat ccgcgatcag gaacgcacaa gaacgaatgt ggtcagcgat tacgcgcagc
     2701 gatttattgc tcagatcggt cgcgccagtg actttcgcta ccgcctggat caacgtgcgg
     2761 aacaggtcga tgtcatagtt agagttaacg tgttgcagca ccgcagcaat acgctccaga
     2821 cccataccgg tatctacaga cggcttcggc agcggttcca tcgtgccatc tgcctggcgg
     2881 ttgaactgca tgaagacgat gttccagatc tcaatgtagc ggtcgccgtc ttcttccgga
     2941 cttcccggag ggccacccca aatgtggtcg ccgtgatcgt agaagatttc ggtgcacggg
     3001 ccgcacggac cagtgtcacc catctgccag aagttgtcag atgcgtatgg cgcacctttg
     3061 ttatcgccga tgcgaataat acgttcgcgc gggatcccta cttctttttc ccagatttcg
     3121 taggcttcgt cgtcgctttc atagacggta acccacagac gctctttcgg cagggcaaac
     3181 catttttcgc tggtcagcag ttcccatgca aactgaatgg catcgtgttt gaaatagtcg
     3241 ccgaagctga agttgcccag catttcgaag aaggtatggt gacgcgcggt gtaaccgaca
     3301 ttttccaggt cgttgtgttt accacccgca cgcacgcagc gttgggaagt ggtagcgcgg
     3361 gaataattac gcttgtcgag tccaaggaac acatccttga actggttcat cccggcgttg
     3421 gtaaacaaca aagttgggtc gttatggggt accagggagc tgctggcaac tacctgatgt
     3481 cccttactat ggaaaaagtc gagaaacgcc tgacggatct cagcggtgct cttgctcata
     3541 attatcctga aatcaagcta acgaaatatc gccaccagct ccagcgtgtc ttaaccgccg
     3601 ggctggtaac tgaaaagtgg gaataagata agttttcttg agtgggaagt aaaataccgt
     3661 atgcgttcag tcggcaaaat ttagccaaat atcctggata tcttccatca gatagccacg
     3721 atagagcaga aaacgctgga tcttaacttt ttctgaaaag acagttggca aaggttcacc
     3781 atattttcgc gtcgcctgat cgcgcaccag tgcgcaccag tcgatgtcac attcacgcat
     3841 cgctttttct gtcgcttcgc gggaaatacc tttctgattc agttcctgac gaatacgtgc
     3901 aggtccataa cctttgcggc tacggctggc gataaagcgc gcaacaaatc ggctgtcatc
     3961 gagatagcca tgttcatggc accaggcaat aacgcgctcg taatcttctg ccgtagcatc
     4021 aatctcttct gggccatttt tgcccataat cggtgccgcg agtttacgtc gcagttcttg
     4081 ctcactgtga tcgcgcaccg ccagaatgcg taccgcacga tccaacaggc gagcatatgc
     4141 cgggcgacgg gatgttgatt ctgtcatggc atatccttac aacttaaaaa agcaaaaggg
     4201 ccgcagatgc gacccaggtt ttgctgataa acagatttaa tagtcaatga gtgtgcagcc
     4261 aattcgttag aggatcgagg tgagagtaat ttttcacgcc agtgttggcc tgttcccctc
     4321 accctaaccc tctccccaaa ggggcgaggg gaccgtccgt gcacacatta tctattatca
     4381 tcaataacga acgattaaaa atcttcgtta gtttctgcta cgccttcgct gtcatctaca
     4441 gagaaatccg gcgttgagtt cgggttgctc agcagcaact cacgtacttt cttctcgatc
     4501 tctttcgcgg tttccgggtt atctttcagc caggcagttg cattcgcttt accctgaccg
     4561 atcttctcac ctttgtagct gtaccacgcg cctgctttct cgatcagctt ctcttttacg
     4621 cccaggtcaa ccagttcgcc gtagaagttg ataccttcgc cgtagaggat ctgaaattca
     4681 gcctgtttaa acggtgcagc gattttgttc ttcaccactt tcacgcgggt ttcgctaccc
     4741 accacgtttt cgccctcttt caccgcgccg atacgacgga tgtcgagacg aacagaggcg
     4801 tagaatttca gcgcgttacc accggtagtg gtttccgggt taccgaacat cacaccaatt
     4861 ttcatacgga tctggttgat gaagatcagc agcgtgttgg actgcttcag gttacccgcc
     4921 agcttacgca tcgcctggct catcatacgt gccgcaaggc ccatgtgaga gtcgccgatt
     4981 tcgccttcga tttccgcttt cggcgtcagt gccgccacgg agtcaacgac gataacgtct
     5041 actgcgccag aacgcgccag ggcgtcacag atttccagtg cctgctcgcc ggtgtccggc
     5101 tgggagcaca gcaggttatc gatatcgacg cccagtttac gtgcgtagat tgggtccagc
     5161 gcgtgttcag catcgataaa cgcacaggtt ttaccttcac gctgcgctgc ggcgatcacc
     5221 tgcagcgtca gcgtggtttt accggaagat tccggtccgt agatttcgac gatacggccc
     5281 atcggcagac cacctgcccc aagcgcgata tccagtgaaa gcgaaccggt agagatggtt
     5341 tccacatcca tggaacggtc ttcacccagg cgcatgatgg agcctttacc aaattgtttc
     5401 tcaatctggc ccagtgctgc cgccaacgct ttctgtttgt tttcgtcgat agccattttt
     5461 actcctgtca tgccgggtaa taccggatag tcaatatgtt ctgttgaagc aattatactg
     5521 tatactcata cagtatcaag tgttttgtag aaattgttgc cacaaggtct gcaatgcata
     5581 cgcagtagcc tgacgacgca ccgcatcacg gtcgccgctg aagcattccc gccgggtaat
     5641 gccttcaccg cgggcagtgg caaaagcaaa ccagacggtg ccgacaggct tctcttcact
     5701 gccgccatcc ggcccggcga taccactaat agacacggca taatcagcac gagccgcttt
     5761 cagtgcacct atcgccattt ccaccacgac gggttcactc accgcgccat gctgcgccag
     5821 cgtctcttcg cgtacgccga tcatctgcgc tttggcttcg ttactgtagg tgacaaatcc
     5881 gcgttcaaac caggcggagc taccggcaat atcggtaatc actttcgcta cccaaccacc
     5941 ggtacaagac tcggcagttg ttacggttgc gccacgggct ttcagcgcct gcccaacctg
     6001 ttcgcttaac tgcatcagtt cactgtcagt catcacaacc tcagtcagtc aaaaatttat
     6061 gccggacaag atagcacttt cgtcaggaag atggtgttgg cgttgcaatt ttacgaggag
     6121 gattcagaaa aaagctgatt agccagaggg aagctcacgc ccccctcttg caaatagtta
     6181 ctgcacgcgc gccagcgcca cggcttgtcc taactgccag accgccattg cgtaatgggt
     6241 gctgtggtta taacgggtaa tggtgtagaa gttcggcaga ccgtaccagt actggtaacc
     6301 ggtaccgaca tccagacgca gcaggctggc ttgttgatgg ttgcccagcg gctgctgtgg
     6361 cgttaaacct gccgtggcaa gctgcgaaat gctgtacctg gttttgaagc catttggcaa
     6421 gcctggagcc tgaccgtttg ccattaccgc gacctgatcg cctttcaccc agccgtgcgc
     6481 tttgaaatag ttcgccacac taccgatcgc atcaaccgga tcccacaggt tgatatgccc
     6541 gtcgccgctg aaatctaccg catattgttt gtaagacgac ggcataaact gtccgtagcc
     6601 catcgccccg gcaaaggaac ctttcagatt gagcggatcg tcctgctcgt cgcgcgccat
     6661 caacaggaag gtttccagct cgccagagaa atactccgcg cggcgtgggt agttaaatga
     6721 cagcgttgcc agcgcatcga ggatgcgagt tttccccatc acgcgccccc agcgggtttc
     6781 aacgccgata atcccgacga taatttccgg cggtacgcca tacacctgcc acgcgcgatt
     6841 caacgcatct tcatactgat tccagaaaac cacaccgttc tgcacgttgt ccggcgtaat
     6901 aaattttttg cgataacgca gccatgcgcc gttcggacct gatggcggtt tcaccgatgt
     6961 ggttggtgcc tggttatcca tcagccgcag taccgaatcc agacgcttcg cctgggagag
     7021 aatttcctgc aactgctgac gatcgaaacc gtgtttgttc accattctgt cgatgaactg
     7081 ctgggcattc gggttattag cgaaatcgcc gcccatctgc atcacattgt gctgaggctc
     7141 aagcaggaag ccgccagacg gcgttccggt ggtcgtttca gtctcagtag gttttggctt
     7201 gctgctacag gcggcaagca acacaaaaag gggaagcaat gttacataac gacgcttgaa
     7261 catgaggggt ccatttaaca gattcaacca ggggcaagta tggtaaagca tcacgccccg
     7321 cacaaggaag cggtagtcac tgcccgatac ggactttaca taactcaact cattcccctc
     7381 gctatccttt tattcaaact ttcaaattaa aatatttatc tttcattttg cggtcaaaat
     7441 aacactttca aatctttcaa tctgattaga ttaggttgcc gtttggtaat aaaacaataa
     7501 atcctgaagg agagaacaat gatagaaacc attactcatg gtgcagagtg gtttatcggg
     7561 ctgttccaaa agggcggaga ggtgtttacc gggatggtga ccggcattct tccgctgttg
     7621 attagcctgc tggttatcat gaacgcactg attaatttta tcggtcagca tcgtattgaa
     7681 cgttttgctc aacgttgcgc cggtaaccct gtttcccgtt acctgctgct accgtgcatt
     7741 ggcacgtttg tcttttgcaa tccgatgacc ttaagcctgg gtcgctttat gccggaaaag
     7801 tacaaaccca gctactacgc ggcagcctct tatagctgcc actcaatgaa tggcctcttt
     7861 ccccatatca accctggcga actgtttgtt tatcttggca ttgccagcgg tctgacaacg
     7921 ctgaacctgc cacttggccc actggcggtg agctatctgc tggttggtct ggtcaccaac
     7981 ttctttcgcg gctgggtgac cgatctgacc accgccattt ttgagaaaaa gatgggcatt
     8041 caacttgaac aaaaagttca cctggcagga gcaacatcat gacgcgtatt cggatcgaaa
     8101 aaggaacggg tggctggggc ggcccgcttg agctggaagc cacgccgggc aaaaaaatcg
     8161 tctatatcac cgccggtacc cggcctgcga ttgttgacaa actggcacaa cttactggct
     8221 ggcaggctat tgacggattt aaagaaggtg aacccgcgga ggcggaaatt ggtgtcgcgg
     8281 taatcgactg tggcggcaca ttacgctgcg gcatctatcc gaaacgacgt attcccacca
     8341 ttaatatcca ctcgacgggc aagtccagtc cgctggcgca gtacattgtg gaagatattt
     8401 atgtctctgg cgtaaaagaa gaaaacatca ctgtagtggg tgatgcgaca ccacaaccct
     8461 cttccgtggg ccgtgactat gacaccagca agaaaatcac cgaacaaagc gatggtttac
     8521 tggcgaaggt gggaatgggt atgggttctg ccgttgccgt gttgtttcaa tctggtcgtg
     8581 acaccatcga cactgtatta aaaaccattc tgccgtttat ggcgttcgtc tcggcgctca
     8641 ttggcatcat tatggcttct ggccttggtg actggattgc ccacggtctt gctccgctgg
     8701 cgagccatcc actaggtctg gtcatgctgg cgctcatctg ctccttcccg ctgctttcac
     8761 ctttcctcgg cccaggcgca gttatcgcac aggttatcgg cgtattgatt ggcgtgcaga
     8821 ttggtctcgg caatattccg ccgcatctgg ctttaccggc actgtttgcc atcaacgcgc
     8881 aggcggcctg cgacttcatc ccggtcggtt tgtcgctggc ggaagcccgt caggacacgg
     8941 ttcgcgtcgg tgtcccttct gtactggtga gccacttttt aaccggcgca ccgactgtac
     9001 tgatcgcctg gtttgtctcc ggttttatct atcaatagag gctgaaacat gaccgttatt
     9061 tatcagacca ccatcacccg tatcggcgcg agtgccactg acgccctcag cgaccagatg
     9121 ctcatcacct ttcgtgaagg cgcgccagcg gacctcgaag agtattgctt cattcattgc
     9181 cacggcgagt tgaaaggtgc actccatccc ggtttgcaat ttacgctcgg gcagcatcgc
     9241 tatccggtga ccgctgttgg cagcgtggcg gaagacaacc ttcgcgaact gggtcatgtc
     9301 accctgcgct tcgatggttt aaacgaagcg gaatttccgg gcactgtcca tgtggcaggc
     9361 cctgtacccg acgatatcgc gccgggatcg gttttgaagt ttgaatctgt taaggagtaa
     9421 aaaatgaatc aggttgtcgt tgtcatcggt ggtgggcaaa ccttaggcgc gttcctgtgc
     9481 cacggtctgg ctgccgaggg gtatcgcgtc gcggttgtcg atattcagag cgacaaagcc
     9541 gcaaatgtgg cacaagaaat taacgccgaa tatggtgaag gaacagcgta tagttttggt
     9601 gctgacgcca ccagcgagca aagcgtcctg gcgctctctc gtggggtaga tgaaatcttt
     9661 gggcgcgtgg atttgctggt ctacagcgcc ggaatagcaa aagcagcctt tatcagcgac
     9721 ttccagctcg gcgattttga ccgtgaatct ggtgggttat ttcctgtgtg cccgtgaatt
     9781 ttctcgtttg atgatccgcg acgggattca gggtcgcatt attcagatca actcgaaatc
     9841 cggcaaagtg ggcagcaaac acaactctgg ctacagcgca gcgaaatttg gtggcgtcgg
     9901 gctgactcaa tcactggcgc tggatctggc ggagtacggc attacggtgc attcactgat
     9961 gctcggtaac ctgctgaaat cgcctatgtt ccagtcactg ttaccccaat acgcgaccaa
    10021 gctgggtatc aaaccggatc aagtcgagca gtattacatc gacaaagtac cgctcaaacg
    10081 cggctgcgat tatcaggatg tgctgaatat gctgctgctc tatgccagtc ctaaggcgtc
    10141 gtactgcacc ggacagtcga tcaatgtcac cggcggtcag gtgatgttgt aatcaacagc
    10201 ggagatccgt taaggatctc cgtgagacta atagaatgcc tgatgcgcta cgctcatcag
    10261 gcatacagga cttccgccac tacattaagg aaaagttatg gtatccgcac tcatcaccgt
    10321 cgccgttatc gcctggtgtg cgcaactggc cttaggcggc tggcaaattt ctcgttttaa
    10381 ccgtgccttc gacacactat gccagcaagg gcgggttggc gtgggccgtt ccagcgggcg
    10441 ctttaaacca cgggtcgtgg tcgccatcgc cctggacgat cagcagcgca tcgtcgacac
    10501 cttgtttatg aaaggactga ccgtcttcgc acgaccgcaa aaaattcccg caattaccgg
    10561 tatgcatgcg ggtgatttac agcccgatgt gatctttccc catgatccac tatcacagaa
    10621 tgctctatca ttggcgctta aactgaaacg tggataattt cgttgtgaat gttacttgct
    10681 tgcgaagtta tcattttgaa acctaaatca ggtaatcacg cccatgaaac ctcgtcagcg
    10741 tcaggccgcc attctggagt atctgcaaaa gcagggtaaa tgctcggttg aagaattggc
    10801 gcaatacttt gacaccacag gcacaaccat tcgcaaagat ctggtcattc tggaacatgc
    10861 aggaaccgtc attcgtactt atggcggagt ggtgttgaat aaagaggaat ccgatccgcc
    10921 tatcgatcat aaaacactca tcaacaccca caagaaagag ctgatagcag aagctgccgt
    10981 tagttttatt catgatggcg attcgatcat tcttgatgct ggcagtaccg ttttgcagat
    11041 ggttcccctg ctctcgcgct ttaataacat cacggtgatg accaacagtc tgcatatcgt
    11101 caatgcgcta tctgaactgg ataacgaaca aactatcctg atgccaggcg gaacgtttcg
    11161 taaaaaatcg gcctcatttc acggacagct ggcagagaat gccttcgaac atttcagttt
    11221 cgataaattg tttatgggca ccgacggcat cgatctcaat gcgggcgtaa ccacctttaa
    11281 cgaggtttat accgtcagta aggcaatgtg caatgccgcg cgcgaagtga ttttgatggc
    11341 ggactcatca aagtttggcc gtaaaagccc caacgtagtt tgcagtcttg aaagcgtcga
    11401 taagctgatt accgacgcag gtatcgatcc ggcgtttcgt caggcgctgg aagagaaagg
    11461 gatcgatgtg atcataaccg gagagagcaa tgagtgaagc actactgaac gcgggacgtc
    11521 agacgttaat gctggagttg caggaagcaa gccgtttacc ggaacgtctg ggcgatgatt
    11581 ttgttcgcgc cgccaatatc atcctgcact gcgaaggcaa agtggtggtt tcgggaattg
    11641 gcaaatcggg ccacattggt aagaaaatcg ccgcaacact tgccagtacc ggcacatcgg
    11701 ctttttttgt ccatccggca gaagcactgc acggcgatct ggggatgatt gaaagccgcg
    11761 atgtgatgct gtttatctct tactccggtg gcgcgaagga actggatctg attattccgc
    11821 gtctggaaga taaatctatc gcgctgctgg cgatgaccgg caaaccgacg tcaccgctgg
    11881 gcctggcggc aaaagcggtg ctggatatct ccgtagaacg cgaagcctgc ccgatgcacc
    11941 ttgcgccgac ctccagcacc gtcaataccc tgatgatggg tgacgcgctg gcgatggcgg
    12001 tcatgcaggc gcgcggattt aatgaagaag attttgcccg ctcccaccca gccggggcac
    12061 tgggcgctcg cttgctgaat aaagtgcatc atctgatgcg ccgtgacgat gccatcccac
    12121 aggtggcgtt aaccgccagc gtgatggatg cgatgctgga actcagccgc accggtctgg
    12181 ggctggtggc ggtatgtgac gctcaacaac aggtacaagg cgtctttacc gacggcgatt
    12241 tacgtcgctg gctggtaggc ggcggcgcac tcaccacgcc agttaatgaa gcgatgacga
    12301 caggcggcac cacgttacag gcgcaaagtc gcgccatcga cgccaaagag atcctgatga
    12361 agcgcaaaat cactgccgca ccggtggtgg atgaaaacgg caaactcacc ggcgcaatta
    12421 acctgcagga tttctatcag gccgggatta tttaatcctt cattcccaga cgtttcgcca
    12481 gccgatgcag gttggcgacg tcggtttcca gcatccgcgc gcaggcagcc cagttatgat
    12541 gattttgtgc cagtgcctgg cgaatagttt cacgctggaa cgcttctgtc gcttcacgca
    12601 ggttttgctt aacaacgggc accgccgccg cttctggcgg cggcaacgtc acctcaggaa
    12661 aagcaaaatg ttgcgcctca agaatcactt catcgccgtt gcgggtggct ctcgccagca
    12721 ctaccgcccg atgaatagca tgttccagtt cgcgcacgtt ccccggaaaa cgatagtgtt
    12781 gcagtaaatt tcgcgctccg gcacttaata ccacgcggga gagccccagc cgcaaacgac
    12841 actgctcgca gaaatacccc gccagcagaa tgacatcatc gccccgctca cgcagcggcg
    12901 gcaccgaaag tggaaacacg ctcaggcgat gaaacaagtc agcgcgaaat cgccctgcca
    12961 tcacctcttc gcgtaagtcg cggttagtcg ccgccagcac gcgcacatcg acccgcaaac
    13021 tacggtcatc gccaacgcgc tgaatatcgc catactgcaa caccctcagc agcttggcct
    13081 gcaatgccaa cgacaactcg ccgatctcat ccagaaacag agtgccgtta tccgccattt
    13141 caaacttccc gctgcggtta ctgatagcgc cagtaaacgc tcctttcaca tgcccgaaca
    13201 actcactttc cgccacactt tccggcagtg cagcacagtt gagatagacc agcggattca
    13261 ccgcccgtgg cgaggcttca tgaatcgctt tcgccaccag ctccttaccg gttcccgtct
    13321 caccgctgat caggacgttg agatcggacg ccgccacaat ctcaatctct tttttcagtt
    13381 gcgtcatgcc aggggacaag ccaatcatct gcgtctgttt caccgcttca aacggcgtgg
    13441 catcgcctgg catcatattc tggctttcca gttgttcaat cagcaacgca ttgcttaacg
    13501 ctcccgccgc cagcgcagca atcagccgta gctcttcgtc gctgaaaaca tcgaactgat
    13561 cgggctgcat cccgtcgagc gtcagtgcgc cgatcaggtt ttgcccggca aacaatggca
    13621 gaccaacgca ggcgtgaacc ttcagactct cctgcccagg aatcaaaccg tcatagggat
    13681 cgggcaattc gctgtctgcg ggaaagcgca ccacatcccc ggcgcgggca atcgcttcca
    13741 gccgtggatg cccttccagc gcaaagcgtc taccgagtac atcctttgcc agaccgtcga
    13801 tggcaagcgg aataaactgc cgcgaatcgt aacgtagcaa cgcagacgca tcgcactcca
    13861 gcacctgacg tagcgtggtg atcaggcgct gaaaacgatc ctgatgacca atcccacgct
    13921 gcaattcgat ggcgatattc gccagcacat caacggaaaa actcatcttt gcctcactgt
    13981 caatttgact atagatattg tcatatcgac catttgattg atagtcattt tgactactca
    14041 ttaatgagca taattttatt tatagagtaa aaacaatcag ataaaaaact ggcacgtaat
    14101 ctgcaattag caagacatct ttttagaaca cgctgaataa attgaggttg ctatgtctat
    14161 tgtggtgaaa aataacattc attgggttgg tcaacgtgac tgggaagtgc gtgattttca
    14221 cggtacggaa tataaaacgc tacgcggcag cagttacaac agctacctca tccgcgaaga
    14281 aaaaaacgtg ctgatcgaca ccgtcgacca taaattcagc cgcgaatttg tgcagaacct
    14341 gcgtaatgaa atcgatctgg cggatatcga ttacatcgtg attaaccatg cagaagagga
    14401 ccacgctggg gcgctgaccg aactgatggc gcaaattccc gatacgccga tttactgtac
    14461 tgccaacgct atcgactcga taaatggtca tcatcatcat ccggagtgga attttaatgt
    14521 ggtgaaaact ggtgacacgc tggatatcgg caacggcaaa cagctcattt ttgtcgaaac
    14581 gccaatgctg cactggccgg acagcatgat gacttacctg acaggcgacg cggtgctgtt
    14641 cagtaacgat gctttcggtc agcactactg cgacgagcat ctgttcaacg atgaagtgga
    14701 tcagacggag cttttcgagc agtgccagcg ttactacgcc aatatcctga cgccgttcag
    14761 ccgcctggta acaccgaaaa ttaccgagat cctgggcttt aacttaccag tcgatatgat
    14821 agcgacctct cacggcgtgg tatggcgcga taacccgacg caaattgtcg agctgtacct
    14881 gaaatgggcg gctgattatc aggaagacag aatcaccatt ttctacgaca ccatgtcgaa
    14941 taacacccgc atgatggctg acgctatcgc ccaggggatt gcggaaaccg acccacgcgt
    15001 ggcggtgaaa attttcaacg tcgcccgaag cgataaaaac gaaatcctga ccaatgtctt
    15061 ccgctcaaaa ggcgtgctgg tcggcacttc gacgatgaat aacgtgatga tgccgaaaat
    15121 cgccggtctg gtggaggaga tgaccggatt acgcttccgt aacaaacgcg ccagtgcttt
    15181 cggctctcac ggctggagcg gcggtgcggt ggatcgtctt tccacgcgcc tgcaggatgc
    15241 gggtttcgaa atgtcgctta gcctgaaagc caaatggcga ccagaccagg acgctctgga
    15301 gttatgccgt gaacatggtc gcgaaatcgc ccgtcagtgg gcgctcgcgc cgctgccgca
    15361 gagcacggtg aatacggtgg ttaaagaaga aacctctgcc accacgaagt ccgacctcgg
    15421 cccacggatg cagtgcagcg tctgccagtg gatttacgat ccggcaaaag gcgagccaat
    15481 gcaggacgtt gcgccaggaa cgccgtggag tgaagttccg gataacttcc tctgcccgga
    15541 atgctctctc ggcaaagacg tctttgacga actggcatcg gaggcaaaat gagcaacggc
    15601 attgtgatca ttggttcggg cttcgccgcc cgccaactgg tgaaaaatat tcgcaaacag
    15661 gacgccagta ttccattaac cctgatcgcc gccgacagca tggatgagta caacaaaccg
    15721 gacctcagcc atgttatcag ccaggggcaa cgtgccgacg accttacccg ccagacggcg
    15781 ggtgaatttg ccgggcagtt taatctgcac ctgtttccgc acacctgggt gacggatatc
    15841 gatgccgaag cccatgtggt gaaaagccag aataatcagt ggcaatacga caagctggtg
    15901 ctggcaactg gtgccagtgc ctttgtcccg ccagtgcctg ggcgtgagtt aatgctgacg
    15961 ttaaatagtc agcaagagta tcgcgcctgt gaaacgcaac tgcgggatgc ccgacgcgtg
    16021 ttgattgtcg gcggtggttt gattggtagc gaactggcga tggatttttg tcgtgcaggc
    16081 aaagcggtca cgctaatcga caacgctgcc agtattctgg cgtcattaat gccaccagaa
    16141 gtaagcagcc gcttgcagca tcggttgacg gagatgggcg ttcatctgct gttgaaatct
    16201 cagttgcagg ggctggaaaa aacggattct ggcattctgg caacgctgga ccaccagcgt
    16261 agtatcgaag tggatgcggt aattgccgcc accggactgc gcccggaaac agccctggca
    16321 cgacgcgccg ggctgacgat taatcgcggc gtttgcgtcg atagttatct gcaaaccagt
    16381 aatgacgata tttatgcgct gggcgattgc gcggaaatca acggtcaggt actgccattc
    16441 ctgcagccga ttcaacttag cgcgatggtg ctggcaaaaa atcttctcgg caataacacg
    16501 ccgctgaaac taccggcgat gctggtgaaa atcaaaacgc cggaattacc gctgcatctg
    16561 gcaggcgaaa cccagcgtca ggatttacgc tggcaaatta ataccgaacg ccagggaatg
    16621 gtggctcgcg gtgttgacga tgctgaccag cttcgcgcct ttgtggtcag tgaggatcgg
    16681 atgaaagagg catttggatt gttgaaaaca ttgccgatgt aggtgggcta ctgtgcctaa
    16741 aatgtcggat gcgacgctgg cgcgtcttat ccgacctacg gggacgcatg tgtaggccag
    16801 ataaggcgtt tacgccgcat ccggcaatgg tgtccaaatg caacacgttt tatccgttct
    16861 ggacttcacc cgctaaccaa cgcgccgcag caataacccc ctgccccaga gacaaaccgc
    16921 catcacccgc cggtaaactc tgtggaaaga gcaatgtgaa atcagcgaga taatgcgcca
    16981 gacgtgcacg cagcaaacgg ttatgaataa ccccgccgct aaataccagc gtagtgatac
    17041 cacgcatcgt ggcctgctca cgcatcaacg cggcaaaacc ctgcgccagc gcatcatgaa
    17101 acgcccacgc gcgttgatta accggtgcct gccagttcag ccactgctgc cagaaagcgg
    17161 cgagatccag ttgattgtcc accagcggca tcgtcaccgg atgcgtcact ccgtggcatg
    17221 aggctgcgag cgcctccaga gcacaagccg cttcaccttc ataacttaac gtggctggcg
    17281 cacagcccag tgccgccgcc actgcatcga acaaccgccc acacgatgac gccagcggcg
    17341 cgttaattcc acgctcaatg gcccgcgcca gcacgctcca gttttgctgt tgcacacttg
    17401 ctgtttcagg gtaattctgc cactccggca caaagcgcag gcactgcgcc agcaggtttc
    17461 gccacggctg cttcgctgcc aaatcgccac ccggaagcgc cactgcaggc aagccgccca
    17521 ggtgctcaca ttcgcgatag ttcacccgca ggcactcgcc gccccacaaa gcgccgttct
    17581 cccccatacc gataccgtcg agcgtcaaag caatgacatc accgccatcc aacggccact
    17641 gatgctctgc cagacacgcc gctgcatggg catgatgatg cagtaccgtt tgcgtcggca
    17701 gattcatttc acgcgcccac tggctggaga catagcccgg atgcgcgtca tgcacaacgt
    17761 attgcggggt aaaatcgtag atgttttgca tcaggcgtaa cgcttcgcgc cactgcatct
    17821 ggatgccatc gtcacttaaa tcgcccagat gctgactcaa caccgcttgt tcaccgcgca
    17881 ccaggcagaa ggtatttttc agatccgcgc cgagacacag cacaggcgga acatttttaa
    17941 agcccggagg caaagccagc gcatccggca catacccccg cgaacggcgc agcatttcgc
    18001 cgctttcgcg caccaccgaa tcatccatcc gctgcacgat gtcgcggtta tgtatcaaga
    18061 atccgtcggc aatgccctgc aaatccgcca gcgcctgttc gttgctgata gctggtggtt
    18121 taccgctcag gttgccggag gtcatcacca gcgggcattg cagttcctgt aacagcaaat
    18181 gctggagcgg gttcgcaggc aacattaccc cgacttcgtt aaggtcaggg gcgatatcat
    18241 cacaaagctc aggaacgtat tttttatcca ccagcacaat cggcgcggcg ggcgtggtaa
    18301 gcaactggcg cgcagcgtct ggtaaaccgt cagccactgg caacatgacc gccagcggtt
    18361 tcgccgggcg atgtttgcgc gcccgaagtg tcgccaccgc gttactgtta cgtgcatcgc
    18421 aggcaagatg aaatccgcca atccctttga tggcgacaat gttgcccatt tttaactgtg
    18481 cgatagccgc ctgtaatgcc gcctcttgtt ctgcatgttc accatgactt acccattcaa
    18541 gatgtgggcc acactccggg caggccaccg gctgggcgtg gaagcgacga tcgagcgggt
    18601 cacggtactc tttgtcacaa gccggacaca acggaaacgc cgccatcacg gtaaacgggc
    18661 ggtcgtaagg catggcgcga ataatggtga aacgcgggcc gcagtgggta cagttgataa
    18721 acggataacg ataacgccgt tcgcctgggg tattcatttc ggcaaggcaa gcagggcaag
    18781 tagcggcatc cgggacaatt tgcgtattca tggcacctcc cgcgctctgg cggatggtga
    18841 actcagtggg cagttgtgac cagatgtacg gctcacgctc gacgctatca atacgcgcca
    18901 gcggcgggca gtgctgatgc aattgaacaa gtaacgtttc cgggtcttcc cgcagccgga
    18961 cttctacgcc atcgccgtca ttacagacat cgccgtgaag atttaattgc tgtgccagct
    19021 gccagacaaa cggacgaaaa ccgacgccct gcactttgcc acgaatacgc agttggacac
    19081 cgcaagatgt gttttttgcc attgagttat tcccgccatc atgaattgcg taacccgtcc
    19141 tgccggacac gactgcgtcg tatccggcag tctcaggtcg gcgataccgc cagctccgta
    19201 ttctacgaat atttccggga attcctttga tgccagaaca gttctgtaag atttttagaa
    19261 catcagcgcc gtacggcggc gtttttctgc actcagttgt tcaagtttat tacgatcgac
    19321 acaaatcagc gcatgagtcg ggcaagccgc catacacgcc gggccgtctt cacgatggtt
    19381 gcacaggtca catttattgg cttcggcttt gtcagcccgt acattcagcc ccgcgccgct
    19441 gttgcgaatc accggacgta ccaccacttc catcgcgcca tacgggcaag ccacaacgca
    19501 ggttttgcaa ccaatgcaac gttcctgcat cacatgaaca aaccctttat cacggctgat
    19561 agcaccattc gggcagacgt tagcgcacgg tgcatcttca cactgacggc aaactgtcgc
    19621 cgtggaaatg ttcacacctt taatgacatg gatacgcggt aaaaatgttt ccggggtcag
    19681 cgatgcacag tcctgatttt cctgatgaga aaccacgcac gccacttcac aggtacagca
    19741 accaatacat ttactcgcgt cagcaatgat gaaacggttc atcaaattct ccagcaatga
    19801 cagttaatgc gccgatacat tcacaaatca tgccagtttt aattcactgt tatttaagga
    19861 aattaatttc tgtaatgcag gaaaaaagat gtcatcgaca ctagtgacga tgacatgtga
    19921 tgacaatgtt tatcgcgaaa gagcaatgag tgagtcgcgg cggatcagtt ttccgctgaa
    19981 ggttttcggc ggtgagaaat ccccgccatc gagcataaaa atcagccgtc caataatttc
    20041 ctgaatcatc tcagtcaccg gaatttttac gctggagagc gccggaacga tatagggggc
    20101 aattgcgata tcatcgaatc cgataactga cacctgctct ggcaccgcta cgccgcgctc
    20161 gtgcaacgct ttcatcgcac ctatcgccat atcgtcgtta ctggcaacta acgcgctaaa
    20221 tttagcccca cgttcgagca acgtttctac cccttcggcc ccgctggcgg gcgtccattt
    20281 accgttagcg ataagttttt cattgagcgc aataccatgc tgcgccagcg cgtctttata
    20341 cccagcaaga cgttcaatgc tggtggggga atccatcgag ccggtaagga aagcaatctc
    20401 ctgatgtccg gcgtttatca actctgccac ggcattaaag ctggtctgtt tatgatcgca
    20461 ccagacgcta tggctgctgt ttttgcgcag gcggcgatta agcaccatta tcggctgact
    20521 gtgcgcgtca atgatgtcat cgatctcatc cacgcttaaa aaacgcgggt aaatcataat
    20581 ggcgtcgcag cgcaaatcca gcagatactg aatcgcctgg cgctcttctt cggcgctgtg
    20641 tttaccatct gccaatagca actgccgccc tttctcttcc gccattcgcg cggcatgaaa
    20701 gagtaattca ctaaaataaa tgccgtggta aagcgtgttg gtcactacca accccagcgt
    20761 ctgagtactc ttcgccgaca gattgcgcgc cagcaagttt gggcggtaac cgctctcttc
    20821 taccgcctga aacacgcgat ctttagtctc ctggctgacg tagccattac ctgaaagcac
    20881 gcgggaaacg gtcgcttttg aaaccccggc gcgcttcgcc acttccagca tcgtcgtcat
    20941 cattttcatc cctttacacg caatcaacgc agtgtactgc accgtttgcc gattgtcctt
    21001 gcgcaatcgg cgggaaaaat attcaggtga ccggtttcac aaatataaaa aatgaacaat
    21061 taactctctt gcttatttag tgacaactat tcatgatttt gtgaaaccgg tttcctaatt
    21121 ccgtttcagc atcggcattt ttccgttacg tcgactgata acaactacat ctaccctact
    21181 gataacagga taaaatccga tggccaaaaa ttatgcggcg ctggcacgct cggtgatagc
    21241 ggcactgggc ggcgttgata acatctcggc ggtcacgcac tgtatgacgc gtttgcgcgt
    21301 tgttatcaaa gatgaccagc ttatcgacag cccgacgtta aaaaccatcc ccggcgtgct
    21361 cggcgtggta cgtagtgaca accagtgtca ggtgattatc ggcaataccg tttcacaagc
    21421 ctttcaggaa gtcgtcagcc tgctgccggg agatatgcag cccgcacagc ccgtgggtaa
    21481 acccaaactc acgctgcgtc gcattggtgc ggggatcctc gatgcgctga tcggcaccat
    21541 gtcaccgctg atcccggcga ttatcggcgg atcgatggtc aaactgctgg cgatgatcct
    21601 cgagatgagc ggcgtgctga caaaaggatc gccgacctta accattctga atgtgattgg
    21661 tgacggtgct ttcttcttcc tgccgctgat ggtcgcggca tctgccgcca tcaaatttaa
    21721 aaccaatatg tcgctggcga ttgccatcgc gggtgtgctg gtacatccga gttttattga
    21781 actgatggcg aaagcggccc agggtgaaca tgtcgaattt gccctgattc cggtcaccgc
    21841 ggtgaaatac acctacacag tgatcccggc gctggtgatg acctggtgcc tgtcatatat
    21901 cgaacgctgg gtggatcgca ttacaccggc ggtgaccaaa aacttcctca agccaatgct
    21961 gattgtgttg attgccgcac cgctggcaat cctgctgatt ggcccgattg gtatctggat
    22021 cggtagcgcc atttcggcgc tggtttacac cattcatggt tatctgggct ggctttcagt
    22081 cgccattatg ggcgcgctgt ggcctctgct ggtaatgacc gggatgcacc gcgtctttac
    22141 gccaaccatc attcagacca ttgccgaaac cggcaaagaa gggatggtca tgccgtcaga
    22201 gatcggcgct aacctgtcgc tgggcggttc atcactggcg gtggcgtgga aaacgaaaaa
    22261 cccggaactg cgccagacgg cgctggctgc ggcggcatca gccattatgg cggggatttc
    22321 cgaaccggcg ttatacggcg tggcgatccg cctgaaacgt ccgcttatcg ccagtcttat
    22381 cagcggtttt atttgcggcg cggttgccgg tatggcgggg cttgccagcc actcaatggc
    22441 agcgccaggg ttatttacca gcgtgcaatt cttcgatccg gcgaatccaa tgagcatcgt
    22501 ctgggtgttc gcggtcatgg cgctggcggt ggtgctgtcg tttatcctca cactgttgct
    22561 cggctttgag gatattcctg ttgaggaagc ggctgccgag gcgcgaaagc atcagagcgt
    22621 acaaccgacc gtcgccaaag aagtaagtct taattgagga tgaaaatgtc agtatttcca
    22681 gaaggttttt tatggggcgg cgcgcttgcc gccaaccagt ctgaaggtgc gttccgtgaa
    22741 ggtggcaaag ggctgaccac tgtcgatatg atcccacacg gcgagcatcg aatggcggtg
    22801 aaactggggc tggaaaaacg ttttcagttg cgcgatgacg agttttatcc cagccatgag
    22861 gcgacggatt tttatcatcg ttataaagaa gatatcgccc tgatggcaga gatgggattc
    22921 aaggttttcc gtacctcaat tgcctggagc cgtctctttc cgcagggcga tgaaatcacg
    22981 cccaatcagc agggcattgc tttttatcgt tctgtctttg aagagtgtaa aaagtacggt
    23041 atcgaaccgc tggtcacgtt gtgccacttc gatgtgccga tgcatctggt caccgaatat
    23101 ggctcctggc gtaaccgcaa gctggtggag tttttcagcc gctacgcccg gacctgcttt
    23161 gaagcatttg atggtctggt gaaatactgg cttaccttca atgaaatcaa cattatgttg
    23221 catagcccgt tctccggcgc gggtctggtg tttgaagaag gtgaaaatca ggatcaggtg
    23281 aaatatcagg ccgcgcatca ccagctggtt gccagtgcgc tagccaccaa aatcgcccat
    23341 gaggttaacc cgcaaaatca ggtgggctgt atgctggcgg gcggtaactt ctacccttac
    23401 agttgcaagc cggaagatgt ctgggcggcg ctggagaaag accgggaaaa cctgtttttt
    23461 atcgatgtgc aggcgcgggg cacgtatccg gcttactctg cccgcgtatt ccgcgaaaaa
    23521 ggggtaacca tcgacaaagc accgggcgat gatgaaatcc tgaaaaacac cgtcgatttt
    23581 gtctctttca gctattacgc ctcgcgctgc gcctcggcgg agatgaacgc caacaatagc
    23641 agtgcggcga acgtagtgaa atcgctgcgt aacccgtatc tgcaagtgag cgactggggc
    23701 tggggtattg atccactcgg tctgcgtatc accatgaaca tgatgtacga ccgttatcag
    23761 aagccgctgt ttctggtgga aaacggcctc ggcgcaaaag atgaacttgc tgccaatggc
    23821 gagattaacg atgactatcg catcagctat ttacgcgaac atatccgcgc aatgggcgaa
    23881 gcgattgcag atggcattcc gctgatgggc tacaccacct ggggctgtat tgatttagtc
    23941 tccgcctcta cgggtgaaat gagcaaacgc tacggttttg tctacgtaga ccgtgacgac
    24001 gcaggcaacg gcacgctgac gcgcacacgt aagaaatcgt tctggtggta taaaaaagtg
    24061 attgccagta atggggaaga tttagagtag gtaacagtgc cggatgcggc gtgaacgcct
    24121 tatccggcct acatgtcgca cgaaccagga aggcccgata agcgcagcgc atcgggcaat
    24181 ctggggtttg gcatcagtca aaatcaaaga aaccattccg gcttaacgcc aaatcgggct
    24241 gaaagtgcct taatgtggga tatcgtcagg gatcgttgtc cagacaaaat ctgactgacc
    24301 agggatttag agccgatttc gttcttcaaa tccgcatagg tcagattatg ttgatcaatc
    24361 agcgtgcgaa gtaacgccac gccaacaggc attgccgcaa cggctttgtc gaactctgca
    24421 aactcttggt tgttgttttc atattctgca atgcggctcg ccagaaagtc gataagtgga
    24481 ttggtgtcat cttcttcaat caaatattcg accagttcca gtgcttcacg ataatcatct
    24541 tctgagtcac tacctcccag aaaggggact gcattgacca gttctcttgt tgctttaaca
    24601 gcgcgtgcag cattagcagt cattctttgt tctctctgta gtaacgtgtc agtttgtcat
    24661 actcggcgtg attagttatg tgcttcacaa agaaccgctt attaacaaag tttatgtagg
    24721 caatcaccct taggttgttc ccacctacat ccagaacgta ccatttgttg cgatatttaa
    24781 aattgtcgag gttgggaaac gccgttcgca tctcttctgg cgtagaaaaa tccgtttcct
    24841 taataacccg atacaatgcc tgaagagcca gagcgtcgtt aggatattta cgcgcagact
    24901 cttcaaatgg cgctttagaa atgatgtgca tttttaagtt ttaacttcct tatagtaagt
    24961 aaacagccac atctgcgagt tctatcctta tgtgttcgga aactatagct catcaaatcc
    25021 aagttcacaa tatgtaaaca cttgagcgcg atataattac ccccaaaatc ctcatagcca
    25081 ctctgcaaaa agtcagttct ggttcacccc atcaagaaca tcccccttta tcaagcaacg
    25141 ctgcgtctgc gccagatctt ccgtttgcgg gtcgcgcccg gcgatgggtt tgaggacgag
    25201 tttacggttg ggggtataag taaaacgcgt gagcgcggga tagcttaact tgccaaacca
    25261 gccgtgaaca cgctgccatt tggcgtcttt tacctgatac agactggtta tccagtaacc
    25321 ggaatcaaac tgcatatcca gcaattgcgt gtgaccattg ccttgcaaat ccagcaaatc
    25381 atctacgcca gttttgcttg cggtgtagaa tcccctgggt tcaaagacac agggacggcc
    25441 ttcgcgcgtg aacgtcataa gaattaaatg tgcataagga gccagaccat taccggtact
    25501 tggtaaccag ataaccagat cctgaatgcc gttacgatca agatcggcgc gatagagttg
    25561 ccctttgccc gctatgtttg ccagcatacc cacaggcaca gaccaggatt taccggcgcg
    25621 atctttaccg ttcaattgcc attcactatc atcatttggc atcttttcaa gcgtcacgcc
    25681 agcaacgccc acttttctaa aatgccaggt ttgctcggca tcataaggca tggtcaggag
    25741 aggtttcagc gcccgctttt gcgcgtattc gctgtcactc attggcagat attcggtaaa
    25801 gctggtggcc tgtgcaggca gcactaagct caataaaaaa gcgcacatcc ctgttctgat
    25861 attcatttcg ctgaattccc tgatggagaa ttattcttct tccaccgcta actgcgcgaa
    25921 gccgccattc ccttcccagc cttccagtcg ttgataaacg gtttctaccg catctttaat
    25981 cggctgagtc atcgggtagt aaaagccgac gatatccggc tggatgccga ggaaaatcac
    26041 ttcgccaatg tcttctttca actggtcgat aagatagttc agcggcatgt tatgggtagt
    26101 catcataaac atctcggcga tatcatccgg gtcgatgatg cggatttcgc cggggtttag
    26161 ccccatatca gtagcgtcaa caatcagtaa ccgcgtaggg cgcagttcac ggatggcgac
    26221 gatgtcgttt tccggtgcac taccgccatc aatcaccacc cagttacctt tcggcgcggc
    26281 ggcgcacttt tccgccagca gcgggccagc gccatcatcg cccatcatgc tattgccaac
    26341 acagagtaaa acgtcagtca cgcagtctcc tcaccatcaa atagatggcg ttttcctgat
    26401 gaatatcatg cagcatactt aacagcattg tactccattc cttctgctgc gaagtttgcg
    26461 ctgcccgggc tttatcgaat gcgttggcca gcatcggcac atggttaatg tcgatgacga
    26521 tctcaccata tttcggcacg ccttccattt tgcggcgggc ttcactgccc gcctccagag
    26581 tggcaatcca tgccagatat tcatcccacg ggcaggtgag cgccgcttcc aggcaatcga
    26641 taaccccaag gtggtgacca atcgccaggc tgtaatagac cacctgctgc gcctcggcgg
    26701 gcgtggcatc gttctcatca ataaatttac ggctcagttg actgaacacc accttttcac
    26761 tcatcggata cgcgcctctt caacaacatg attcagatgg ctgacaatct cgttcagacg
    26821 cggatcgttt tccgcttcca gccagcgtgc aacctgttct tcgccctgcc ctaactgtgt
    26881 caggaagtca tcggcaatct gacgaccgta acgataaccc gccaggcggc gtgcttcgcg
    26941 atccactttc acacgcagcg gctgcaccat atcgccatgc aggatttccg cctgttgttc
    27001 atcctgctca cccgggccac gggcgtgaat tttctgctcc agcaggccga gcgccattgc
    27061 aaagccgtac agcgtggcgg caggcgttgg cgggcaacca ggaatgtaga catctaccgg
    27121 gacgatttta tccgtaccgc cccacacgca gtagagatcg tggaaaatac cacctctgtt
    27181 accgcaggca ccgtaggaga tacaaatttt cgggtccggc gcggactgcc acgcacgcag
    27241 cgcaggggat cgcattgcac gggtgaccgc gccggtaaac agtaaaatat ccgcatgacg
    27301 cggtgaagga acgactttaa tgccgaagcg ttctgcatca aacagcggcg aaagcgtggc
    27361 gaaaatttcg atttcgcagc cgttgcagcc gccgcagtcc acgcgataaa cgtaggcaga
    27421 acgtttgatt tttttcagta acgacgcctt catgctggcg atggattcat ccaccgtcat
    27481 cgggaccgga atgccgttgg cgtcacgggg gcctaataaa ttgctcatca gatggcctct
    27541 ttcatatggc gagtcagttc aatacggtcg gacggcacca ggcatttctg gcgcttacat
    27601 tccgggcaag tctcaaagct ttcgcggtgg ttttccgcgc ggctgtcgcc gttgtgctta
    27661 agcagcgcaa tggcgtagtc gatctctttc tggacggcga aaggacgatt gcagacgcgg
    27721 cagttgcaca gcgcgaagcg ggactgttgc aggaagtctt ctttcttcca cactgccagt
    27781 tcgtactctt gcgacagttt gatcgccgcc gtcgggcaaa cttcttcgca gcgtccacag
    27841 aagatgcagc gcccgagatt aaactcccac gccagctcac cggtggcgag gtcagtttca
    27901 accgttaagg cgtttgacgg gcaggcattg acgcaggccg cacagccgat gcactgctgc
    27961 gggttctgct ccggcttacc acggaagttt ttatcaaccg caatcggctc cagcggataa
    28021 gacgaggtcg ccgtgccggt tttgatgact tttttgataa aggtaaacat ggcgattcct
    28081 tatttcagcg gcgagttttt acgctcaatg ctgtaacgct cgagttcttt gtacggcacc
    28141 actttgctct ttttcttacg cacatcgacc acggtcatgc ggtcggtaca ggagtagcaa
    28201 gggtccaagc taccgataat cagcggcgca tcggaaacgg tgttaccgcg cagcatgtag
    28261 cgcagggtcg gccagttcgc gtaggtcgcg gcacggcagc gccagcggta cagcttctgg
    28321 ttgtcgccgg tcatgctcca gtggatatcg tcgccgcgcg gcgcttcggc aaagcccagc
    28381 gcgaaacggt gcggaatgta ggtaaagcct tccaccatca gcggaccgcc cggcaggtta
    28441 tccagaccgt agtcgatcat gttcagcgcg gtataaactt cgttgatacg cactttcaga
    28501 cgggaaataa cgtcgcagcc ctgctcgctg tggacttcca ttggcagcag gccataaccg
    28561 acaaacgggt gatcggcgcg agtatcgcgg gcgtgaccgc tggcgcgaac catcgggcca
    28621 acgttactga aatcgcgggc catttccggg tccagacgac caatgccgac ggtgcgctgt
    28681 tccatgttcg gcgtactcag cagcacatcc accagctcct gcacttcacg acgcatctgt
    28741 tgcgccagct ggcgggtctg gatcatgtca tctttcaaca gatcgcgacg aatcccgccg
    28801 atcaggttca ggccgtaggt tttacgcgca ccggtgagga tctctgccat tttcatggag
    28861 gtttcacgca cgcggaagaa ctgcataaag ccggagtcaa agccggtaaa gtggcaggcg
    28921 agaccgaggt tgagcagatg cgagtgcagg cgttccacct ccagcagaat ggcgcggatc
    28981 atctgcgcac gttctggcac ctgaataccc atcgcgtttt ctaccgacgt ggtgtaggcg
    29041 gtgctgtggg caaagccgca gatcccgcac acacggtcag agaggaaggt cacttcgttg
    29101 taacccatac gggtttccgc cagtttttcc atgccgcgat gaacgtagaa cagacggtag
    29161 tcggcgtcga taatgttttc gccatcgacg aacagacgga agtggcccgg ttcgtcagaa
    29221 gtgacgtgca gcggaccaat cggcacgacg ttgtttttct tgtcgcccag ttcgttgatg
    29281 aactcgtagg tttcagcatc ggtggtcggt gccggacgct gacgataatc catgctgtct
    29341 ttacgcagcg gataaagttc atccggcagc accagacggc gttcatccgg cagaccaacc
    29401 ggaatcaaac cgtacatatc gcgcacttcg cgctcgcccc acaccgccgc cggaacgcgc
    29461 ggcgtcacgg acggatactc cggtttgttg gcatcaactt cgacgcgaac cgttacccag
    29521 cacttagtcc ccttctccat cgacagcacg taataaacgg cgtaatgacc attcagtttg
    29581 cgttcgtcgt taccaaaaag caccgacagc cagcccccct gcttgtagta gagaaactcc
    29641 accacttccg gcagatagtt cacctttacg gtgacagtca gctgatcttt ggtctgccag
    29701 gcgtggtcca gcacgacgcc cggaaatgcc tcattcagcg cggcgagata atgttgacct
    29761 aatttttctt cagacatgct caaactctct ttaatcacgc cgccagcaag gagacgaacg
    29821 ctaaaaatgc aaagccaaac ccagtccagg taatgcgcgg agtaatatca agacgcagac
    29881 gggccatgct gttttcgaac agcgcgataa ccaggacgcc gacgaccagt ttgacgatgg
    29941 caatcaccag cgccagcagc agcccaccgg cggtgaaggt ttccatctgt ccccacggga
    30001 taaatacgcc gacgaacatc tgcagcacca ccagctgttt caggctgata ccccatttca
    30061 tgacgccaaa gccgctgccg ctgtattcag agagcgggcc ttcctgcagc tcctgctcgg
    30121 cttccgccag gtcgaacggc agtttgccca tttcgataaa ggtggcgaac gcacaggcac
    30181 aaagcgccag taccagcggg atgctctggc tcagcggcca gtgataaacg gtgtcggtga
    30241 tgttgctgat gttggtggaa ccggcaacct gtgcggcaac ccacagacca agcagcagca
    30301 tcggttcgac cagcacgcca agcatcgctt cacggctcgc gccgatagcg gtaaacgggc
    30361 taccggtatc cagaccagaa atggcaaaga agaaacgcgc gatggcaaag agatacagta
    30421 aggtgattaa atcacccagt tgcggcagcg gagaaccgac ggtcaccacc ggcagcgcag
    30481 tagcgatagt cagcatgacg cccaccatca catacggcgt caggcggaac acccagccgg
    30541 aggcatccgg gccaacgctc tggcggccca gcagtttgaa aatgtcacga tactcctgca
    30601 acacacccgg cccacgacgg ttatgcagac gggcgcgcgc cacgcgggta ataccggaga
    30661 gcagcggcgc aacggcaaat aacaccagcg cctgaattaa cggatataaa acactcattc
    30721 tcaggctcct cgtgaaacaa taatcaccac cagtaccgcc agttcaacca gcgccatccg
    30781 gcggaacagc aacgcactac cttcgcactg ccagcccggc accagcgaca ccggattcag
    30841 ccatttgcgc agtttcagca ctggcgcaaa cgcctgttta accggcatgg caaaaccgtg
    30901 agcggtaatc accattgatt tttcatggtc gtagccgcac acccaggccg caccgcggga
    30961 acgtgacggc aggcggtcgc ctttgcaaat cgccataatg atgaatggca gcagcgggca
    31021 ggcaatcagc agcaatgtga tcattggttg agaaacggtg gtgttagcag gctccagagg
    31081 cagaggtaca gcagcagaga gcatcggcag cagccacggc gcagcaacac cgccaattac
    31141 gcagcaaatc gccagtgcca ctacgcttac gctcatcagg agcggcgcac aggtggcgtt
    31201 ttcggcttct ttggtacgcg gcgcgccgag gaacgtgacg ccatagactt tcgccataca
    31261 catcaccgcc agcgcaccgg taattgccag acccacagcg agcagcggcc ccagcagacg
    31321 ggcaacaaac gcgccgctat tgctcagttt gaagaaggat tgatagataa cccattcccc
    31381 ggcaaacccg ttcagtggcg gcagcgcagc cattgccatc aacccgacta acatggcgat
    31441 ggagataacc ggcattttct tgccaatacc accgaatttt tcgatatcgc gatgaccggt
    31501 acggaaccag acgctccccg ccccgaggaa cagtacgctt ttgaacaggc tatggttaag
    31561 cagatggtac agaccaccga ccaggccaag agcaatcagc gccggttgtt cgagcgcgat
    31621 acccgttacg ccagcgccca gccccagcag gataatgccg atattttcca gggtgtggta
    31681 agccagcagg cgctggatgt tgtgttccat caacgcatac agaccaccga cgaacgcggt
    31741 gatcatgccg agcaccagca gcgcgatccc ccaccacagc ggtgcattac cgcccagcag
    31801 tgacagggtt aacatgccca gcaggccaat tttcatgact accgtagaaa acaacgcggc
    31861 agctggcgca gaggcgttag catgtgcctg cggcacccag ccgtgcagcg gaataatccc
    31921 ggccagcagg ccaaagccaa tcacaccgag cagccagata tcggaaccga gcggcagctg
    31981 ttgcatacgc atatccagta ggcacagatc cagcgtgcca taacgctgcc acagcagcca
    32041 gcaagcaatc accagcagca gagtgccaag acgccccagc gcaaaccaca gtttgccctc
    32101 tttgctgttg ctggtgagga acaccgcaca caaggccatg acttcggcca ttaccacgaa
    32161 catgccgagg ttgctggcga tgacggcgca gacggcggca gccatcaaca tattgatctg
    32221 caagccgttg cacttcacct gcgcgtggcg atgccagtca atgttgtaga ggctgacaaa
    32281 cagaccgcac agaccgagcg taatcagcca aatcgcgtta agcggagaga tttgcacatc
    32341 gtagcttacc agcgacagcg caccgctcac gccaaccgcg ccagtcagta cagtgaagcc
    32401 cgcggctgcc gtatacagac taccaaccgc gccgccaatt ccagctatcc agccacttaa
    32461 cgctttttga aaagaaaaga gaaatgccag aacagcggcg gcgacaaacc atgccacgcc
    32521 gctattgatc agggaaattg cgctcattta gcctctccac tttgagcctg ctgaaacaag
    32581 gtgagatcgc caaagtccgt gttaaaggtc agctcacgct tacgtttgct gacgcgggcg
    32641 atatcggtgt tatccaccag atgcagggct ttagtcgggc acatccgcac gcaggccgga
    32701 ccttgttcat caaaactgca aaggtcacat ttgacggcga tcgcgcgaat acccggcacc
    32761 cagtcaagca gcgtactgat acgcgccgga gcaggcggtg ccggtggcgc tttcggggta
    32821 ttggcgtttg ccggaatatc cagcggacgg ctgccggaaa attcaattgc gccaaacggg
    32881 caggcgatgc cgcacagttt gcagcttacg cacagacttt cattcaactg cacggcccca
    32941 tcaatgcggg tgatggcgtt aaccgggcaa accaccgcac agggtgcatc ttcacagtgg
    33001 tgacagagct gcggcgcaga ttctttttca ttcagcatca ctctcaggcg cggcattgat
    33061 tgcaggccgt gctggcgatg cgtctctgaa caggcggcct cacaagtgtg gcagccgata
    33121 cagagcgtgg agtcaacaat tacaaaacga ttcaccaggc attcctcagg tgattgtcat
    33181 ttttgacgaa aacatgccga tgaaatgtca ttttcgacac tcatcgacac gcccatcccc
    33241 aaacaggcgt aacgcctgca aaacgggcaa agcctcagct catgctgccg ggctttgtcc
    33301 ctttaccagt tggcttaaat tcaccggcac attgttttca atagcggtgt ataagctgtc
    33361 gtaaacgcca gcgatttttt cgagatagtg ttccagaacc tgttcgcgat cgcggatgtt
    33421 gaccttgcca tcaatcatgc cgtcaatgct cagttgcgcc tgcgcaatcc gctgtttgtc
    33481 ttcgccatct tttgtttcga cgtaatactc gatggtgtgg ctgttaaagc catccgccag
    33541 ggtgacgtaa atacgaaaat gttcaaaaag gacgaaacag agttctttgt ccggcgcgca
    33601 gctcatggcg tgatgcaggc gcgctttcga taacgtgatc ccctgaacca gcgaattgca
    33661 gtagatgtgc cactggtcct gtaggcgaca atgccgctgt gcgatgtaat cggctttctc
    33721 gcttatttcc caaatagtca ttgtcaggtt acccgtttaa cagagatgcc agctttaagc
    33781 attttctatg ccaactttta atttattgtt attaaataga tttttaagct aaagatgaat
    33841 ttcgtcgccg tgtcgacgtg tcatttcgac atcatcgaca ttattcaccg cagggataat
    33901 caacactggc acaattattg cttgtagctg gcaatagtta atgggaggcg atatgcacga
    33961 aataaccctc tgccaacggg cactggaatt gatcgaacag caggccgcaa aacacggcgc
    34021 aaaacgcgta actggggtct ggctcaaaat tggcgcattt tcttgtgtcg aaaccagctc
    34081 tcttgccttt tgttttgatc tggtttgccg cggcagcgtg gcggaaggtt gtaaactgca
    34141 cctcgaagaa caagaggccg aatgctggtg tgaaacatgc caacagtatg tgacgctact
    34201 gacccagcgc gtccgccgct gtccacagtg tcatggtgac atgctacaga ttgtggcaga
    34261 cgacggttta cagattcggc ggatagaaat agaccaggag tgagcgatgt gtacaacatg
    34321 cggttgcggt gaaggcaacc tgtatatcga gggtgatgaa cataaccctc attccgcgtt
    34381 tcgtagcgcg ccatttgccc cggcggcacg cccgaagatg aaaatcaccg gcattaaagc
    34441 gcctgaattt acccccagcc agactgaaga aggcgacctg cattacggtc atggcgaagc
    34501 gggcactcac gcgccgggca tgagccagcg tcggatgctg gaagtcgaaa ttgacgtgct
    34561 ggacaaaaat aaccgtctgg ctgaacgcaa ccgcgcgcgc tttgctgccc gcaagcaact
    34621 ggtgctcaac ctggtttcca gccctggttc cggtaaaacc accctgctga cggaaaccct
    34681 aatgcgcctg aaagacagcg ttccgtgcgc ggttattgaa ggtgaccagc aaaccgtgaa
    34741 cgatgccgca cgcattcgcg ctaccggcac accagcgatt caggtgaaca ccggtaaagg
    34801 ctgccatctt gacgcacaga tgattgccga cgccgcaccg cgtctgccac tggacgataa
    34861 cggtattctg tttatcgaaa acgttggcaa cctcgtatgc ccggccagct tcgatctcgg
    34921 tgaaaaacac aaagtggcgg tgctttccgt taccgaaggt gaagacaaac cgctgaaata
    34981 tccgcatatg tttgccgccg cctcgctgat gctgctcaac aaagttgacc tgttgccgta
    35041 tctcaacttt gacgttgaga agtgcatcgc ctgcgcccgc gaagtcaatc cagaaattga
    35101 aatcatcctt atttccgcca ccagcggcga agggatggac cagtggctga actggctgga
    35161 gacacagcga tgtgcatagg cgttcccggc cagatccgca ccattgacgg caaccaggca
    35221 aaagtcgacg tctgcggcat tcagcgcgat gtcgatttaa cgttagtcgg cagctgcgat
    35281 gaaaacggtc agccgcgcgt gggccagtgg gtactggtac acgttggctt tgccatgagc
    35341 gtaattaatg aagccgaagc acgcgacact ctcgacgcct tacaaaacat gtttgacgtt
    35401 gagccggatg tcggcgcgct gttgtatggc gaggaaaaat aatgcgtttt gttgatgaat
    35461 atcgcgcgcc ggaacaggtg atgcagttaa ttgagcatct gcgcgaacgt gcttcacatc
    35521 tctcttacac cgccgaacgc cctctgcgga ttatggaagt gtgcggtggt catacccacg
    35581 ccatttttaa attcggcctc gaccagttac tgcctgaaaa cgttgagttt atccacggtc
    35641 cgggttgccc ggtgtgcgta ctgccgatgg gtagaatcga cacctgcgtg gagattgcca
    35701 gccatccgga agtcatcttc tgtacctttg gcgacgccat gcgcgtgccg gggaaacagg
    35761 gatcgctgtt gcaggcaaaa gcacgcggtg ccgatgtgcg catcgtttac tcgccgatgg
    35821 atgcgttgaa actggcgcag gagaatccaa cccgtaaagt cgtattcttc ggcttaggtt
    35881 ttgaaaccac catgccgacc accgccatca ccctgcaaca ggcgaaagcc cgcgatgtgc
    35941 agaattttta cttcttctgc cagcatatta cgcttatccc gacgctgcgc agtttgctgg
    36001 aacagccgga taacggtatc gacgcgttcc tcgcgccggg ccacgttagt atggttatcg
    36061 gcactgatgc ctataatttt atcgccagcg attttcatcg tccgctggtg gtcgctggtt
    36121 tcgaacccct tgatctactg caaggcgtgg tcatgctggt ggagcagaaa atagcggccc
    36181 acagcaaggt agagaatcag tatcgtcggg tggtaccgga tgccggtaac ctgctggcgc
    36241 aacaggcaat tgctgatgtg ttctgtgtca acggcgacag cgaatggcgc ggcttaggcg
    36301 tgattgaatc ttctggcgtg cacctgacgc cggattatca acgattcgat gccgaagcac
    36361 atttccgccc agcaccgcag caggtctgcg atgacccgcg cgcgcgttgt ggtgaggtat
    36421 taacgggcaa atgtaagccg catcaatgcc cgctgtttgg taacacctgt aatcctcaaa
    36481 ccgcatttgg tgcactgatg gtctcctccg aaggagcgtg cgccgcgtgg tatcagtatc
    36541 gtcagcagga gaatgaagcg tgaataatat ccaactcgcc cacggcagcg gcggccaggc
    36601 gatgcagcaa ttaatcaaca gcctgtttat ggaagccttt gccaacccgt ggctggcaga
    36661 gcaggaagac caggcacgtc ttgatctggc gcagctggta gcagaaggcg accgtctggc
    36721 gttctccacc gacagttacg tcattgaccc gctgttcttt cctggcggta atatcggcaa
    36781 gctggcgatt tgcggcacag ccaatgacgt tgcggtcagt ggcgctattc cgcgctatct
    36841 ctcctgtggc tttatcctcg aagaaggatt gccgatggag acactgaaag ccgtagtgac
    36901 cagcatggca gaaaccgcgc gcgcggcagg cattgccatc gttactggcg atactaaagt
    36961 ggtgcagcgc ggcgcggcag ataaactgtt tatcaacacc gctggcatgg gcgcaattcc
    37021 ggcgaatatt cactggggcg cacagacgct aaccgcaggc gatgtattac tggttagcgg
    37081 cacgctcggg gaccacgggg cgactatcct taacctgcgt gagcagctgg ggctggatgg
    37141 cgaactggtc agcgactgcg cggtactgac gccgcttatt cagacgctgc gtgacattcc
    37201 cggcgtgaaa gcgctgcgtg atgccacccg tggtggtgtg aacgcggtag ttcatgagtt
    37261 cgcggcagcc tgcggttgtg gtattgaaat ttcagaagtg gcactgccgg ttaaacctgc
    37321 cgtgcgcggc gtttgcgaac tgctgggact ggacgccctt aactttgcca acgaaggcaa
    37381 actggtgatc gccgttgaac gcaacgcggc agagcaagtg ctggcagcgt tacattccca
    37441 tccactgggg aaagacgcgg cgctaattgg tgaagtggtg gaacgtaaag gtgttcgtct
    37501 tgccggtctg tatggtgtga aacgaaccct cgatttacca cacgccgaac ctcttccgcg
    37561 tatatgctaa taaaattcta aatctcctat agttagtcaa tgaccttttg caccgctttg
    37621 cggtgctttc ctggaagaac aaaatgtcat atacaccgat gagtgatctc ggacagcaag
    37681 ggttgttcga catcactcgg acactattgc agcagcccga tctggcctcg ctgtgtgagg
    37741 ctctttcgca actggtaaag cgttctgcgc tcgccgacaa cgcggctatt gtgttgtggc
    37801 aagcgcagac tcaacgtgcg tcttattacg catcgcgtga aaaagacacc ccattaaata
    37861 tgaagacgaa actgttctgg cacacggtcc ggtacgccgc attttgtcgc gccctgatac
    37921 gctgcattgc agttacgaag aattttgtga aacctggccg cagctggtcg caggtgggct
    37981 ataccccaaa tttggtcact attgcctgat gccactggcg gcggaagggc atatttttgg
    38041 tggctgtgaa tttattcgtt atgacgatcg tccctggagc gaaaaagagt tcaatcgtct
    38101 gcaaacattt acgcagatcg tttctgtcgt caccgaacaa atccagagcc gcgtcgtcaa
    38161 caatgtcgac tatgagttgt tatgccggga gcgcgataac ttccgcatcc tggttgccat
    38221 taccaacgcg gtgctttccc gcctggatat ggacgaactg gtcagcgaag tcgccaaaga
    38281 aatccattac tatttcgaca ttgacgatat cagtatcgtc ttacgcagcc accgtaaaaa
    38341 caaactcaac atctactcca ctcactatct tgataaacaa catcccgccc acgaacagag
    38401 cgaagtcgat gaagccggaa ccctcaccga acgcgtgttc aaaagtaaag agatgctgct
    38461 gatcaatctc cacgagcggg acgatttagc cccctatgaa cgcatgttgt tcgacaccta
    38521 gggcaaccag attcaaacct tgtgcctgtt accgctgatg tctggcgaca ccatgctggg
    38581 cgtgctgaaa ctggcgcaat gcgaagagaa agtgtttacc actaccaatc tgaatttgct
    38641 gcgccagatt gccgaacgtg tggcaatcgc tgtcgataac gccctcgcct atcaggaaat
    38701 ccatcgtctg aaagaacggc tggttgatga aaacctcgcc ctgaccgagc agctcaacaa
    38761 tgttgatagt gaatttggcg agattattgg ccgcagcgaa gccatgtaca gcgtgcttaa
    38821 acaagttgaa atggtggcgc aaagtgacag taccgtgctg atcctcggtg aaactggcac
    38881 gggtaaagag ctgattgccc gcgcgatcca taatctcagt gggcgtaata atcgccgcat
    38941 ggtcaaaatg aactgcgcgg cgatgcctgc cggattgctg gaaagcgatc tgtttggtca
    39001 tgagcgtggg gcttttaccg gtgccagcgc ccagcgcatt ggtcgttttg aactggcgga
    39061 taaaagctct ctgttcctcg acgaagtggg cgatatgcca ctggagttac agccgaagtt
    39121 gctgcgtgta ttgcaggaac aggagtttga acgtctcggc agcaacaaaa tcattcagac
    39181 ggacgtacgt ttaattgccg cgactaaccg cgatctgaaa aaaatggtcg ccgaccgaga
    39241 gttccgtagc gatctctatt accgcctgaa cgtattcccg attcacctgc cgccactacg
    39301 cgagcgtccg gaagatattc cgctgctggc gaaagccttt accttcaaaa ttgcccgtcg
    39361 tctggggcgc aatatcgaca gcattcctgc cgagacgctg cgcaccttga gcaacatgga
    39421 gtggccgggt aacgtacgcg aactggaaaa cgtcattgag cgcgcggtat tgctaacacg
    39481 cggtaacgtg ctgcagctgt cattgccaga tattgtttta ccggaacctg aaacgccgcc
    39541 tgccgcaacg gttgtcgccc aggagggcga agatgaatat cagttgattg tgcgcgtgct
    39601 gaaagaaacc aacggcgtgg ttgccgggcc taaaggcgct gcgcaacgtc tggggctgaa
    39661 acgcacgacc ctgctgtcac ggatgaagcg gctgggaatt gataaatcgg cattgattta
    39721 actgcaaatt gccggacaga tctgcctgtc cggcatacta ttcacgaggt tttttcggac
    39781 gatatttttc cggcagttct ggtaccggac gcttgtcatc gatgagatga cgcacggtta
    39841 agatcggatg acgccacagc attctcggcc cggcccaacg cataatctgt ttcatctctt
    39901 cacgctttgc aggctggtaa cagtgcaccg gacactgctt acaggctggt ttctcttcgc
    39961 cgaacacaca tttatccagc cgcttttgcg cgtaaacaaa caacgcctcg taatgctccg
    40021 gctccgctga cgcctgcggg catttcgctt gataaagatc gatcattttt ttaatcgtca
    40081 gtttttcacg agagatacgc ttgccggaca tgctgcctcc atactcttaa ggtgtattta
    40141 tattacaact taatttgaaa ggggactatg gctctgaagg aggaggttta ttcaaccgac
    40201 gcaattttgc gtacttgctt cataagcatc acgcaaaagc tgcaaaacag catctttccc
    40261 ggaaccagca tcaagaactc gccgttcgct tcttcccctg aaatgattaa ctccggtatc
    40321 atgtgcgcct tatgtgatta caacgaaaat aaaaaccatc acaccccatt taatatcagg
    40381 gaaccggaca taaccccatg agtgcaatag aaaatttcga cgcccatacg cccatgatgc
    40441 agcagtatct caggctgaaa gcccagcatc ccgagatcct gctgttttac cggatgggtg
    40501 atttttatga actgttttat gacgacgcaa aacgcgcgtc gcaactgctg gatatttcac
    40561 tgaccaaacg cggtgcttcg gcgggagagc cgatcccgat ggcggggatt ccctaccatg
    40621 cggtggaaaa ctatctcgcc aaactggtga atcagggaga gtccgttgcc atctgcgaac
    40681 aaattggcga tccggcgacc agcaaaggtc cggttgagcg caaagttgtg cgtatcgtta
    40741 cgccaggcac catcagcgat gaagccctgt tacaggagcg tcaggacaac ctgctggcgg
    40801 ctatctggca ggacagcaaa ggtttcggct acgcgacgct ggatatcagc tccgggcgtt
    40861 ttcgcctgag cgaaccggcc gaccgcgaaa cgatggcggc agagctgcaa cgcactaatc
    40921 cggcggaact gctgtatgca gaagatttcg ccgagatgtc gctgattgaa ggccgtcgcg
    40981 gcctgcgccg tcgcccgctg tgggagtttg aaatcgacac cgcgcgccag cagttgaatc
    41041 tgcaatttgg cacccgcgat ctggtcggtt ttggcgtcga gaacgcgccg cgcggacttt
    41101 gtgctgccgg ttgtctgttg cagtatgcga aagataccca acgcacgacc ctgccgcata
    41161 ttcgttctat caccatggaa cgtgagcagg acagcatcat tatggatgcc gcgacacgtc
    41221 gtaacctgga aattactcag aacctggcgg gcggtgcgga aaatacgctg gcttctgtgc
    41281 tcgactgcac cgtcacgccg atgggtagtc gtatgctgaa acgctggctg catatgccag
    41341 tgcgcgatac ccgcgtgttg cttgagcgcc agcaaactat tggcgcattg caggatttca
    41401 ccgccgagtt gcagccggta ctgcgtcagg tcggcgacct ggaacgtatt ctggcacgtc
    41461 tggctttacg aactgctcgc ccacgcgatc tggcccgtat gcgccacgct ttccagcaac
    41521 tgccggagct gcgtgcgcag ttagaaactg tcgatagtgc accggtacag gcgctacgtg
    41581 agaagatggg cgagtttgcc gagctgcgcg atctgctgga gcgagcaatc atcgacacac
    41641 cgccggtgct ggtacgcgac ggtggtgtta tcgcatcggg ctataacgaa gagctggatg
    41701 agtggcgcgc gctggctgac ggcgcgaccg attatctgga gcgtctggaa gtccgcgagc
    41761 gtgaacgtac cggcctggac acgctgaaag ttggctttaa tgcggtgcac ggctactaca
    41821 ttcaaatcag ccgtgggcaa agccatctgg cacccatcaa ctacatgcgt cgccagacgc
    41881 tgaaaaacgc cgagcgctac atcattccag agctaaaaga gtacgaagat aaagttctca
    41941 cctcaaaagg caaagcactg gcactggaaa aacagcttta tgaagagttg ttcgacctgc
    42001 tgttgccgca tctggaagcg ttgcaacaga gcgcgagcgc gctggcggaa ctcgacgtgc
    42061 tggtgaacct ggcggaacgg gcctataccc tgaactacac ctgcccgacc ttcattgata
    42121 aaccgggcat tcgcattacc gaaggtcgcc atccggtagt tgaacaagta ctgaatgagc
    42181 catttatcgc taacccgctg aatctgtcgc cgcagcgccg tatgttgatc atcaccggtc
    42241 cgaacatggg cggtaaaagt acctatatgc gccagaccgc actgattgcg ctgatggcct
    42301 acatcggcag ctatgtaccg gcacaaaaag tcgagattgg acctatcgat cgcatcttta
    42361 cccgcgtagg cgcggcagat gacctggctt ccgggcgctc aacctttatg gtggagatga
    42421 ctgaaaccgc caatatttta cataacgcca ccgaatacag tctggtgtta atggatgaga
    42481 tcgggcgtgg aacgtccacc tacgatggtc tgtcgctggc gtgggcgtgc gcagaaaatc
    42541 tggcgaataa gattaaggca ttgacgctgt ttgccaccca ctatttcgag ctgacccagc
    42601 taccggagaa aatggaaggc gtcgccaacg tacatctcga tgcgctggag cacggcgaca
    42661 ctatcgcctt tatgcacagc gtgcaggacg gcgcggcgag caaaagctac ggtctggcgg
    42721 ttgctgctct ggcgggcgta ccgaaagagg ttattaagcg cgcacggcaa aaactgcgtg
    42781 agctggaaag catttcgccg aacgccgccg cgacgcaagt ggatggtacg cagatgtctt
    42841 tgctgtcagt gccagaagaa acttcgcctg cggtcgaagc actggaaaat ctcgacccgg
    42901 attcactgac tccgcgtcag gcgctggagt ggatttatcg cttgaagagt ctggtgtaat
    42961 aacaattccc gatagtcttt tgctatcggg aatattaacg acaactgacg aatcaaataa
    43021 aaataccctg tataatagga aagcttattt tacagggtaa aaccatgcca tctacacgct
    43081 atcaaaaaat caatgcccat cactatcgcc atatatgggt cgttggtgat attcatggtg
    43141 aatatcagtt attacaatcc cgttaacatc aactctcttt ttaccctgaa accgacttac
    43201 ttatttccac aggcgataat attgatcgtg ggccgaagag tcttaacgtg ctgcgcctgc
    43261 taaaccaacc ctggtttacg tcggttaaag gcaaccacga agcgatggcg ttggatgcat
    43321 tcgaaactgg cgatggaaat atgtggcttg ccagcggtgg tgactggttt ttcgatttaa
    43381 atgattcaga gcaacaagag gcaatagatc tgttgctgaa attccatcac cttccacata
    43441 ttattgaaat cattaatgac aacataaaat atgccatc
//
LOCUS       DAJVQG010000013        43365 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJVQG010000013 DAJVQG010000000
VERSION     DAJVQG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43365
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.013"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..210)
                     /locus_tag="OQM83_000691"
     CDS             complement(<1..210)
                     /locus_tag="OQM83_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCS1519031.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKT"
     gene            complement(413..649)
                     /gene="feoC"
                     /locus_tag="OQM83_000692"
     CDS             complement(413..649)
                     /gene="feoC"
                     /locus_tag="OQM83_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCS1519032.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(649..2970)
                     /gene="feoB"
                     /locus_tag="OQM83_000693"
     CDS             complement(649..2970)
                     /gene="feoB"
                     /locus_tag="OQM83_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCS1519033.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(2987..3214)
                     /gene="feoA"
                     /locus_tag="OQM83_000694"
     CDS             complement(2987..3214)
                     /gene="feoA"
                     /locus_tag="OQM83_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCS1519034.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(3652..5973)
                     /locus_tag="OQM83_000695"
     CDS             complement(3652..5973)
                     /locus_tag="OQM83_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCS1519035.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6070..6546)
                     /gene="greB"
                     /locus_tag="OQM83_000696"
     CDS             complement(6070..6546)
                     /gene="greB"
                     /locus_tag="OQM83_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCS1519036.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            6774..7493
                     /gene="ompR"
                     /locus_tag="OQM83_000697"
     CDS             6774..7493
                     /gene="ompR"
                     /locus_tag="OQM83_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCS1519037.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7490..8842
                     /gene="envZ"
                     /locus_tag="OQM83_000698"
     CDS             7490..8842
                     /gene="envZ"
                     /locus_tag="OQM83_000698"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCS1519038.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(8918..10540)
                     /gene="pckA"
                     /locus_tag="OQM83_000699"
     CDS             complement(8918..10540)
                     /gene="pckA"
                     /locus_tag="OQM83_000699"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCS1519039.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            10919..11233
                     /locus_tag="OQM83_000700"
     CDS             10919..11233
                     /locus_tag="OQM83_000700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCS1519040.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FG"
     gene            11246..12619
                     /locus_tag="OQM83_000701"
     CDS             11246..12619
                     /locus_tag="OQM83_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCS1519041.1"
                     /translation="MAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNG
                     LTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALVVILARTQSRLIDSIQ
                     KLFTLIATGLLPLVSLLTLMFIITLPFTDLSAISRHISAAGLLLTLAFLQLILMAIVR
                     DPQKASLPWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVL
                     LVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMA
                     RYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQ
                     EQVSEKVLAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQA
                     ERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLDLTVDGET
                     LEINLSK"
     gene            complement(12754..13632)
                     /gene="hslO"
                     /locus_tag="OQM83_000702"
     CDS             complement(12754..13632)
                     /gene="hslO"
                     /locus_tag="OQM83_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCS1519042.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(13657..14058)
                     /gene="hslR"
                     /locus_tag="OQM83_000703"
     CDS             complement(13657..14058)
                     /gene="hslR"
                     /locus_tag="OQM83_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCS1519043.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14069..14737)
                     /gene="yrfG"
                     /locus_tag="OQM83_000704"
     CDS             complement(14069..14737)
                     /gene="yrfG"
                     /locus_tag="OQM83_000704"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCS1519044.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(14802..16937)
                     /gene="igaA"
                     /locus_tag="OQM83_000705"
     CDS             complement(14802..16937)
                     /gene="igaA"
                     /locus_tag="OQM83_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCS1519045.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            17257..17817
                     /gene="nudE"
                     /locus_tag="OQM83_000706"
     CDS             17257..17817
                     /gene="nudE"
                     /locus_tag="OQM83_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCS1519046.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(17982..20534)
                     /gene="mrcA"
                     /locus_tag="OQM83_000707"
     CDS             complement(17982..20534)
                     /gene="mrcA"
                     /locus_tag="OQM83_000707"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCS1519047.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            20654..21433
                     /gene="hofM"
                     /locus_tag="OQM83_000708"
     CDS             20654..21433
                     /gene="hofM"
                     /locus_tag="OQM83_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCS1519048.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21433..21972
                     /gene="hofN"
                     /locus_tag="OQM83_000709"
                     /pseudo
     CDS             21433..21972
                     /gene="hofN"
                     /locus_tag="OQM83_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
     gene            21956..22396
                     /gene="hofO"
                     /locus_tag="OQM83_000710"
     CDS             21956..22396
                     /gene="hofO"
                     /locus_tag="OQM83_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCS1519049.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            22386..22790
                     /gene="hofP"
                     /locus_tag="OQM83_000711"
     CDS             22386..22790
                     /gene="hofP"
                     /locus_tag="OQM83_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCS1519050.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            22702..23940
                     /gene="hofQ"
                     /locus_tag="OQM83_000712"
     CDS             22702..23940
                     /gene="hofQ"
                     /locus_tag="OQM83_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCS1519051.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24341..24862
                     /gene="aroK"
                     /locus_tag="OQM83_000713"
     CDS             24341..24862
                     /gene="aroK"
                     /locus_tag="OQM83_000713"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCS1519052.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            24919..26007
                     /gene="aroB"
                     /locus_tag="OQM83_000714"
     CDS             24919..26007
                     /gene="aroB"
                     /locus_tag="OQM83_000714"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCS1519053.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26099..27385
                     /gene="damX"
                     /locus_tag="OQM83_000715"
     CDS             26099..27385
                     /gene="damX"
                     /locus_tag="OQM83_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCS1519054.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            27492..28328
                     /gene="dam"
                     /locus_tag="OQM83_000716"
     CDS             27492..28328
                     /gene="dam"
                     /locus_tag="OQM83_000716"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCS1519055.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28346..29023
                     /gene="rpe"
                     /locus_tag="OQM83_000717"
     CDS             28346..29023
                     /gene="rpe"
                     /locus_tag="OQM83_000717"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCS1519056.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29016..29774
                     /gene="gph"
                     /locus_tag="OQM83_000718"
     CDS             29016..29774
                     /gene="gph"
                     /locus_tag="OQM83_000718"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCS1519057.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            29767..30771
                     /gene="trpS"
                     /locus_tag="OQM83_000719"
     CDS             29767..30771
                     /gene="trpS"
                     /locus_tag="OQM83_000719"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCS1519058.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            30931..31836
                     /locus_tag="OQM83_000720"
     CDS             30931..31836
                     /locus_tag="OQM83_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1519059.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            31853..32215
                     /locus_tag="OQM83_000721"
     CDS             31853..32215
                     /locus_tag="OQM83_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCS1519060.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            32299..33462
                     /locus_tag="OQM83_000722"
                     /pseudo
     CDS             32299..33462
                     /locus_tag="OQM83_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            33462..34688
                     /locus_tag="OQM83_000723"
                     /pseudo
     CDS             33462..34688
                     /locus_tag="OQM83_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            34685..35563
                     /locus_tag="OQM83_000724"
     CDS             34685..35563
                     /locus_tag="OQM83_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCS1519061.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            35574..35927
                     /locus_tag="OQM83_000725"
     CDS             35574..35927
                     /locus_tag="OQM83_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCS1519062.1"
                     /translation="MKKIGVAGLQREQIKKTIEATATGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            35939..37243
                     /locus_tag="OQM83_000726"
     CDS             35939..37243
                     /locus_tag="OQM83_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCS1519063.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            37255..38340
                     /locus_tag="OQM83_000727"
     CDS             37255..38340
                     /locus_tag="OQM83_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCS1519064.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(38492..39223)
                     /gene="phoB"
                     /locus_tag="OQM83_000728"
     CDS             complement(38492..39223)
                     /gene="phoB"
                     /locus_tag="OQM83_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCS1519065.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(39323..40108)
                     /gene="frlD"
                     /locus_tag="OQM83_000729"
     CDS             complement(39323..40108)
                     /gene="frlD"
                     /locus_tag="OQM83_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCS1519066.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40105..40936)
                     /gene="frlC"
                     /locus_tag="OQM83_000730"
                     /pseudo
     CDS             complement(40105..40936)
                     /gene="frlC"
                     /locus_tag="OQM83_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            complement(40986..42008)
                     /gene="frlB"
                     /locus_tag="OQM83_000731"
     CDS             complement(40986..42008)
                     /gene="frlB"
                     /locus_tag="OQM83_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCS1519067.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42029..>43365)
                     /gene="frlA"
                     /locus_tag="OQM83_000732"
     CDS             complement(42029..>43365)
                     /gene="frlA"
                     /locus_tag="OQM83_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HCS1519068.1"
                     /translation="GSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWLT
                     VLAFVIGELIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDAP
                     SLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAKI
                     IPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMTG
                     EIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPALG
                     STAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSII
                     LQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGLM
                     TTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
BASE COUNT        10061 a        11049 c        11903 g        10352 t
ORIGIN      
        1 cgttttcacc gaccacagca catcggaata gctttcacgc agatcgtcat caataaagct
       61 gctcgactcc agcttgaggg ttttcatatc acaaagcgcg tgaatgggtg ccggtaaatg
      121 aaacgcaaga aaatcacgag ccgtgtccgg ttggcgtaaa aagagtttga acagcgcatc
      181 gtgtggggtg gaactctgct ttttgctcat ggcgttcctt cacctgttgt ttgaatgccg
      241 cgactctacg cgccactcca cacctgtgca ttagcgagtt ttcatccctg cgagatgctt
      301 tcaaaggaac ttacgccgtg ctgcaagagt tgcattttta tggaaagccg cacgcaaaaa
      361 agcccggatg cagacgcata ccgggctttc caaaacaggc gatggagtaa ggttaacgca
      421 gcgcccacca ctcgcgcaga caggcttttc cttccgggca gcttttacaa ctgccagaga
      481 ggcagccgtc aggttcttcc tgaatccgca cggctttgcc catactttcc agttgttgca
      541 gcatggcgtt aatcattggc tgcggagtgt tcaatgtctg gcttatctgg gtcgcttcca
      601 tacggccccg taacgccagc aaatcacgta cctgaataag tgaagccatt aatggcaatc
      661 accggtggtg ctggctgcgc agcaactgct taccgacttg cgggttgcca gcagttcgat
      721 atccacccgg ctacgcgcgc ggcgcagcag accgataact acgatgttaa acaggattac
      781 cgccagaatg cacaccaggc tgtaagttgg gtgctgactg tagctggcga cctgatagaa
      841 caatgttgcc agtgagtaag cgatattcag cccccacagg atggagaagc ccatccagcc
      901 acggcttgat tcacgggcga tggctcccat caccgagata catggtacat acagcaggac
      961 gaaaatcagg tagctgtatg cggcagctgc gctaccgaat ttctgatcca tcacgcccat
     1021 cgccccggta cccatttcac cgtcgccttt gctagcttca atggggttca tcagtacgct
     1081 aaggctgaag gtgtctttca ggctctgcca ggtttcatct accgcactga acagctcttc
     1141 accgaggttg aattccgccg gattgaactc ttcgtcctga atattttctg cggtgtagag
     1201 ggtgttgagc gtacccacca ccacttcttt tgccattgcg cccgtaaaca ggccaaccgt
     1261 tgcctgccag ttatcttcat gtacgccaat cggcttgaag acaggggtta tcacccggct
     1321 gacggacgcc agcgccgagt cgttgatgtt atcgacgatt ttcccgctca acgagaagct
     1381 gttgaaagcg ctcaggaaaa tgctgacgat gataatcact ttaccagcac gcagaacgaa
     1441 gcccttcaga cgctgccagg tctggataat caggctttta acgtgtggta catggtagac
     1501 cggcagctcc atgacaaacg gcgtcgcttc accgcgcatg atggtgtact tgagcatcag
     1561 gccagtcagc accgccatca cgataccgag catatacagc gagaaaaccg ccagggcacc
     1621 gttctgcccg aagaaggcag ccgcgaatac tgcgaagata gccagacgcg cgccgcagga
     1681 cataaacggt gccatcatga tggtcatcag acgttcacgc ggtgcatcaa gcgtacgtgc
     1741 gcccattacc gacggtacgt tacaaccgaa accgacgatc agcggcacaa aggatttccc
     1801 cggcaagccc agcgcctgca tcaggcggtc catcacaaac gctgcacgcg ccatatagcc
     1861 ggagtcctca aggaaggaga ggaacaggta catcatgccg atctgcggca ccagtggcag
     1921 cacggtgtta ataccgccac ccaggccctg ggcgaggaag atagtcagcc agtccgggaa
     1981 gtggagcgtg tagccaatcc attgaatacc atgcacaaat agcgccacgg agccgacgtc
     2041 aaacagcggc tgtaacgccc cgccgatgtt gatagccagc aggaacatca ggtacatcac
     2101 aaagaggaaa atcggcagac cgaggaaacg gttgagcacg attttatcta ccgcagtggt
     2161 gaaacggctg ggttctgccg tcagggtgtt gcttaccaca tcacagatgg cagctatgca
     2221 ctggtaacgc gcatcggcaa tgtgcagcgc cggatcatcc atttcattac gcagacgggc
     2281 aagggcggca tccagatgct gcgacgcttc accggcgtag gcgcggctgt agatatcgcc
     2341 ttccagcatt tgcaggccca gccagcgacg ttgtttcagc gggatgtcgg aaggcatcac
     2401 ttttgccagt gaatctgctt cgttgagtag cggctgtgcg taatgcacca gttccacatt
     2461 ctcgttagct ttatagcgat caatcgccag cttgagcgct tcaataccgc gaccacgggt
     2521 tgaaaccagc gggatcactg ggcagccaag acgcgccgac agagcatcaa tttcaatacg
     2581 aatattttgc ttctcggcaa tgtcgagcat gttcagtgcc acgatgcagg gaatgccgag
     2641 ttccagcagt tgtagcgtca ggtacaggtt acgctcaagg ttagacgcat ccaccacgtt
     2701 aatcagcagg tcggcgtcgc cactcaaaat gtagtgacag gcgatttgct catcgagcga
     2761 ggtctgcgat gagatggtgg tcagagaata ggtgccgggc aggtccacca gcgtgacctg
     2821 atgatcggtg gtggagaatt gcccttcttt acgttcgacg gtaacgccag cccagttacc
     2881 tacacgctga cgtgcgctag tgagctggtt aaataaggtt gtcttgccag aatttggatt
     2941 accaattaag ccaatggtta attttttcat tgttgttatc accgtattaa caggaaaccg
     3001 cttccacttc taataaggcc agatcttttt tgcgtaatac caggctcaca cgacgggttt
     3061 cgatatgaat ggggtcgccg agtggagcga cgcgcaccac attaaaagag gagccaggta
     3121 acatgccaag agaaagcagt ttttggcgat atgccgggct gatttcacgg gaaaagccag
     3181 tgattttcca cgcagtatct ggagtgtatt gcataggtgc ctacttgttt ctcattaact
     3241 ggataactac ctcatggggc gtttgacagc gacatgagac cagatgattt gccaacaaag
     3301 gagagattaa ataatcaaca acacaatgat aatgagaatg gtttctatca gcaatactta
     3361 aaatgtgtgc gaatgttggt ttgaataata atctgactca atgttgatgt ggctcaaggt
     3421 tatatcgcag cgaaaatata attcgaatag tgaattaccc ggactggcta atgtttaatt
     3481 aaggtttcag ttgttaatga agtgataaat attgccggaa tatattatga gtttggttgt
     3541 ggaataaaaa ggccggagat attctccggc acttttatgt caggcaaggc gttcgttgcc
     3601 agatgcggcg tgaacgcctt atcgcgcagg tttaggggcc agtccgggcg tttaacgttt
     3661 tttgcccatt gccgccgcca gcgcatccat catcgcgcta ttaccggcag gctgcgcttc
     3721 acgaccgcgt ggtttggctg ccgggcggtt gttttgcggg cgttcattac cgccgccgcg
     3781 acgggcgttg gtttcgccag gctgctcgtc cagacgcatg gtcagggcga tacgtttacg
     3841 ctgaagatcc acttccagca ctttcacctt cacaatgtcg cccgctttca ccacggtatg
     3901 cggatcttcc acaaacttgt tcgacaatga agagatgtga accaggccgt cctgatgcac
     3961 gccaatatcg acaaacgcgc caaagttggt gacgttggtg acagcgcctt cgaggatcat
     4021 acccggttgc aggtcgttca ttgtctcgac gccatcggca aactgagcgg ttttaaattc
     4081 cggacgcgga tcgcgacccg gtttttccag ctctttgatg atgtcagtta ctgtcggcac
     4141 accgaatttt tcatcagtaa agtcagacgc tttcaggtta cgcagttcgc tgctgttacc
     4201 catcagatct ttcagtgcct gctgtgttgc tgccagaatg cgttccacca ccggataggc
     4261 ttccgggtga acggtagagg cgtccagcgg gttatcaccg tggttaatgc gcaggaagcc
     4321 cgcgcactgc tcgaaggctt tcggccccag acggctgact ttcagcagtt gctgacggtt
     4381 cttgaactgg ccgttctcat cgcgccaggc cacgatgttt tgcgccatca tgcgcgtcag
     4441 gcccgccacg cgagttaata gcggaacaga agcggtgttg agatcgacgc caacggcgtt
     4501 tacgcagtct tctactactg catccagttt gcgggccagt tgcgtctggc tgacgtcatg
     4561 ctgatactga cctacgccga tagatttcgg atcgattttc accagctccg ccagcggatc
     4621 ctgcaaacgg cgggcgatag ataccgcacc acgcagcgaa acgtcgagat ccgggaactc
     4681 ctgcgctgcc agctcggaag ctgagtaaac cgacgcgcca gcttcgctga cgatcacttt
     4741 ctgtgcggtc actttcggga actgcttctg cacgtcgaga tagaaacgtt cagtttcgcg
     4801 ggaagctgta ccgttgccga tcgccaccag ttcaacgtta tgtttttcac acaaggcagc
     4861 aacggtcatc gctacttttg cggcttgtcc ggtgtgcggg taaatggtat cggtcgccac
     4921 cagtttgcca gtggcatcga ccactgccac ttttaccccg gtacgcagac ccggatcgag
     4981 gcccatcgtt gcacgcagtc cggcaggggc cgccatcagc agatcgtgca ggttacgggc
     5041 aaagacgttg attgcttcat cttccgcgcg ttcgcgcacg gtacccatca gttcggtttc
     5101 cagatgcatc agcaccttga tgcgccaggt ccagctcact acgcctttgc gccagctatc
     5161 cgccggggca ttgttcaggc gcaggccaag gtgatccatg atgatttgct cgcaatagct
     5221 ctctttgggc ggctcatcga actgcggatc ggcattcagc gaaagctgga gtacgccttc
     5281 gttacgccca cggaacatcg ccagcgcgcg gtgagaaggc accgtggaca acggttcgtg
     5341 atgatcgaaa tagtcgcgga atttcgcccc ttcctcttct ttaccgctca ccaccgtaga
     5401 aaccaaatgc gcgttcttcc acagataatc acgcactttc gccagcagcg cggcatcttc
     5461 ggcaaaccgt tccatcagga tatagcgcgc gccgtccagc gcggctttgg tatctgccac
     5521 gcctttatcg gcatcaatat attgcgcagc ggcgacttct ggtgtgtgtg acggatcgct
     5581 ccacagcagg tcagccaacg gctcaagccc tgcttcaatg gcgatttgcc cgcgggtgcg
     5641 gcgtttaggt ttgtagggca ggtagaggtc ttcgagttcg gttttgctta gggtggcgtt
     5701 gatggccttc gccagatcat cggtgagttt gccttgctcg gaaatggact tgaggatcgc
     5761 ctgacgtctc tcttccagct cgcgcagata gctcagacgc gtttccagat tacgcagctg
     5821 cgtgtcatcc agaccgccgg tgatttcctt acgataacgt gcgataaacg gcacggtatt
     5881 cccttcgtca agcaggcgaa cggcagcgtc aacctgttcc gggcgcgcct gaatttcacc
     5941 cgcaataatg cggcagaacg aatcattcat catggtttga gttcatcttt tcggatcaaa
     6001 aatcagggga tagttatacg gactggctgg caaaaatgcc agccatcggc aggaggttaa
     6061 gactcttcat tacggtttca cgtattcgat agcattaaca taccagctcg cttccccggc
     6121 aggggtattc accaccgcca gatcgccgac ttcttttttc agcaatgcgc gggccatcgg
     6181 ggaatcgata gagatgtaat ctttacggcc aaaaatttca tcgtagccga caatacggaa
     6241 acggtgagtc acgccatcgt cgttttcaat ctccacccac gcgccaaaaa agactttgcc
     6301 ttcctgctga ggggagtaat tgacgatttt gagattttcc atgcatttag tgagatagcg
     6361 cacgcgacgg tcgatttcac gcagacgctt tttattatac tgatagtcag cattttcgct
     6421 gcggtcgccc agacttgcgg cccaggtcac cttttttgtg acctccgggc gttcttcacg
     6481 ccagagataa ttaagctctt gtttgagttt ttcataccct tcccgggtaa ccaggggcgt
     6541 tttcatctcg ttgattccct ttgtctgttt gataatgcgc acattgggta taacgtgatc
     6601 atatcaacag aatcaataat gtttcgccga ataaattgta tacttaagct gctgtttaat
     6661 atgctttgta acaatttagg ctgaaattca taccagattt agctggtgac gaacgtgagc
     6721 ttttttaaga atacacgctt acaaattgtt gcgaaccttt gggagtacaa acaatgcaag
     6781 agaactacaa gattctggtg gtcgatgacg acatgcgcct gcgtgcgctg ctggaacgtt
     6841 atctcaccga acaaggcttc caggttcgaa gcgtcgctaa tgcagaacag atggatcgcc
     6901 tgctgactcg tgaatctttc catcttatgg tactggattt aatgttacct ggtgaagatg
     6961 gcttgtcgat ttgccgacgt cttcgtagtc agagcaaccc gatgccgatc attatggtga
     7021 cggcgaaagg ggaagaagtg gaccgtatcg taggcctgga gattggcgct gacgactaca
     7081 ttccaaaacc gtttaacccg cgtgaactgc tggcccgtat tcgtgcggtg ctgcgtcgtc
     7141 aggcgaacga actgccaggc gcaccgtcac aggaagaggc ggtaattgct ttcggtaagt
     7201 tcaaacttaa cctcggtacg cgcgaaatgt tccgcgaaga tgagccgatg ccgctcacca
     7261 gcggtgagtt tgcggtactg aaggcactgg tcagccatcc gcgtgagccg ctctcccgcg
     7321 ataagctgat gaaccttgcc cgtggtcgtg aatattccgc aatggaacgc tccatcgacg
     7381 tgcagatttc gcgtctgcgc cgcatggtag aagaagatcc agcgcatccg cgttacattc
     7441 agaccgtttg gggcctgggc tacgtctttg taccggacgg ctctaaagca tgaggcgatt
     7501 gcgcttctcg ccacgaagtt catttgcccg tacgttattg ctcatcgtca ccttgctgtt
     7561 cgccagcctg gtgacgactt atctggtggt gctgaacttc gcgattttgc cgagcctcca
     7621 gcagtttaat aaagtcctcg cgtacgaagt gcgtatgttg atgaccgaca aactgcaact
     7681 ggaggacggc acgcagttgg ttgtgcctcc cgctttccgt cgggagatct accgtgagct
     7741 ggggatctct ctctactcca acgaggctgc cgaagaggca ggtctgcgtt gggcgcaaca
     7801 ctatgaattc ttaagccatc agatggcaca gcaactgggc ggcccgacgg aagtgcgcgt
     7861 tgaggtcaac aaaagttcgc ctgtcgtctg gctgaaaacc tggctgtcgc ccaatatctg
     7921 ggtacgcgtg ccgctgaccg aaattcatca gggcgatttc tctccgctgt tccgctatac
     7981 gctggcgatt atgctattgg cgataggcgg ggcgtggctg tttattcgta tccagaaccg
     8041 accgttggtc gatctcgaac atgctgcttt gcaggtcggt aaagggatta ttccgccgcc
     8101 gctgcgtgag tatggcgcgt ccgaggtgcg ttccgttacc cgcgccttta accatatggc
     8161 ggctggtgtt aagcaactgg cggatgaccg cacgctgctg atggcggggg taagtcacga
     8221 cttgcgcacg ccgctgacgc gtattcgcct ggcgactgag atgatgagcg agcaggatgg
     8281 ctatctggca gaatcgatca ataaagatat cgaagagtgc aacgccatca ttgagcagtt
     8341 tatcgactac ctgcgcaccg ggcaggagat gccgatggaa atggcggatc ttaatgcagt
     8401 gctcggtgag gtgattgctg ccgaaagtgg ctatgagcgg gaaattgaaa ccgcgcttta
     8461 ccccggcagc attgaagtga aaatgcaccc gctgtcgatc aaacgcgcgg tggcgaatat
     8521 ggtggtcaac gccgctcgtt atggcaatgg ctggatcaaa gtcagcagcg gaacggagcc
     8581 gaatcgcgcc tggttccagg tggaagatga cggtccggga attgcgccgg aacaacgtaa
     8641 gcacctgttc cagccgtttg tccgcggcga cagtgcgcgc accattagcg gcacgggatt
     8701 agggctggca attgtgcagc gtatcgtgga taaccataac gggatgctgg agcttggcac
     8761 cagcgagcgg ggcgggcttt ccattcgcgc ctggctgcca gtgccggtaa cgcgggcgca
     8821 gggcacgaca aaagaagggt aaataaacgg gaggcgaagg tgcctcccgt tttgctttct
     8881 ataagatact ggatagatat tctccagctt caaatcatta cagtttcgga ccagccgcta
     8941 ccagcgcggc acccgcaggg gtgtcggtgt atttatcgaa gttgtcgata aacagtttcg
     9001 ccagggtttc ggctttttcc tgccactgtt ccggagaagc gtaggtgtta cgcggatcga
     9061 gaatcttcgt gtctacgccc ggcagttcgg ttgggatcgc caggttaaac atcggcagag
     9121 tgaaggtttc tgcattatcc agcgaaccgt tgaggatggc gtcgataatg gcgcgggtat
     9181 ctttaatcga gatacgtttg ccagtgccgt tccagccagt gttaaccaga taagcctgcg
     9241 cgcccgccgc ctgcatacgt ttcaccagca cttctgcgta ctgagtcggg tgcagcgaca
     9301 ggaacgccgc gccgaagcaa gcggagaagg ttggcgtcgg ttcggtgatg ccacgctcag
     9361 taccggccag tttggcggtg aagccagaga ggaagtgata ctgggtttga tcggcagtca
     9421 ggcgagaaac cggcggcaac acgccgaaag catcagcagt caggaagata accttagtcg
     9481 cgtggcccgc tttggaaacc ggcttaacga tgttatcgat gtgatagatt ggataagaaa
     9541 cgcgggtgtt ctcggttttt gaaccatcat caaagtcgat agtgccatct tcacgcacgg
     9601 tgacgttttc cagcagcgca tcacgacgga tagcgttgta gatttcaggt tccgcttctt
     9661 tcgacagctt gatagttttt gcgtagcagc cgccttcgaa attaaacacg ccgtcatcgt
     9721 cccagccgtg ttcgtcatcg ccaatcaggc ggcgtttcgg gtcggtggaa agggtggttt
     9781 taccggtgcc ggaaaggccg aagaacaccg caacatcgcc tttctcgcca acgttggcgg
     9841 agcagtgcat agaagcgata cctttcagcg gcagcaggta gttcatcatc gagaacatcc
     9901 ctttcttcat ttcgccgccg taccaggtgc cgccaatcag ctgcatgcgc tcggtcaggt
     9961 taaacgccac gaagttttcg gagttcagac cctgttcttt ccactgcggg ttagtgcact
    10021 tcgcgccgtt cataacgata aagtctggtt tgaaacctgc cagctcttca tcgctcgggc
    10081 gaataaacat gtttttgaca aaatgcgcct gccaggccac ttcggtgatg aaacggacgg
    10141 aaagacgagt atccgggttc gcaccacaga aagcgtcgac aacgaacaga cgtttgccgg
    10201 aaagctgcct ggtcaccagg cctttcagat gctgccaggt ttccggagag agaggtttgt
    10261 tgtcgttctt acctttgcct ttgtctgccc accagaaagt atcgcgagtg gtatcgtcac
    10321 ggacgatata cttatctttt ggtgaacgac cggtgaagat cccggtatcg acggcaacgg
    10381 cacccagatt agttaacacc ccgcgctcat aacctgtcag gctcggatcg agctcttcct
    10441 gatacagcag gtcgtagctt gggttgtaaa cgatatcatg tacgtcactg ataccataag
    10501 cctcgagttc ttgcggggtc aaaccattgt taacgcgcat ttcactgctc cttagccaat
    10561 atgtattgcc tgaatagtaa agtctttttg ggggtgttaa ccgcgacaag gctcatagat
    10621 ttacgtatct ggagaaattc atatttgaca aaaaactccg tgattcctgt cacgaaacgg
    10681 ttgctattat cgcagaaata gcattttagg tgtggaaaat gttctcaaaa ggttaaatct
    10741 tggtttatat ggcgggaata ttgtgagtgg aatcgcattc ctgaatgaaa ttgatgcaaa
    10801 ttcaaccggt aaagtatcga ttcaccacgc taactttttt tgattatttt ctgaaaaaag
    10861 caaaatacta ctgcccggat gcgataatta acccaccttt tgattctgga taaccaacat
    10921 ggataacgtt gaactttcac cggcaacacg ctgggggatg attgctaccg gattacttca
    10981 ggggctggtt tgctacctgc ttatcgcctg gcttgccgga aaaaatcaca gttggattgt
    11041 ttatggcgtg cctgcgaccg tcgcgttttc atccgtttta ctcttctccg tgatctcttt
    11101 taaacagaag cgcctttggg gatggctggc gctggtgttt attgccacga cagggatgag
    11161 cggttggctg aagtggcaaa ctgatggcat gaacccctgg agagctgaaa aggcgatttg
    11221 ggattttggc tgatatctgc tgttgatggc aatgttgttg ctaccgtgga tacaacaaag
    11281 ccttcgtatc cgcaatggca gtagccgcta tagctacttt taccagtcag tatggcataa
    11341 cgtacttata ttactggtga tttttctctc caatggcctg acgtggctgg tgcttttact
    11401 gtggggtgag ttgtttaaac ttgttggcat cacgtttttc aatacgctct tttttgcaac
    11461 cgactggttt atgtatctta catttggtct ggttaccgca ctggtggtga tcctcgcgcg
    11521 tacacagtca cgtttaatcg actctattca aaagttgttc acgctaatcg ccacggggtt
    11581 gctgccgtta gtatcattgc taaccctgat gtttatcatc accctgccgt ttacggacct
    11641 gagcgcgatt tctcgccaca tctccgccgc cgggttgctg ttaacgctgg cctttttgca
    11701 attgatctta atggctattg tccgcgatcc gcaaaaagcg tcacttccct ggacggggcc
    11761 gttgcgttgc ctgattaaaa ccgctttgct ggttgctccg ctgtatgtgt tcgtcgccgc
    11821 ctgggcgtta tggctgcggg tcgctcagta cggctggact gtcgaccgct tgcagggcgc
    11881 gctggcggtg ctggtgttac tggtgtggtc gctggggtat tttgtcagta tcgtctggcg
    11941 taatgggcaa aatccccttg ttcttcaggg caaagtgaac cttgcggttt cgttattggt
    12001 gttggtgata ctggtgcttc ttaattcgcc ggtgctggac agtatgcgca ttagcgtgaa
    12061 cagtcatatg gcgcgttatc agagcggcaa aaacacgcca gaccaggtaa ctatctacat
    12121 gctcgaacag agcggtcgct atggacgtgc ggcgcttgag tcgctgaaaa gcgatgccgg
    12181 gtttatgaaa gacccgaaac gcgcgcggga tctgctgatg gcgttagatg gagagcaaca
    12241 tcttcaggaa caggtatcgg aaaaagtatt agccgaaaat gtgttaattg ctcctggttc
    12301 tgttaaaccg gatgcgacat tctggtcggc cttaatccag gatcgctata acgtgatgac
    12361 ctgtattgaa aaagacgcct gcgtcctggt cgagcaagat ctgaatagtg atggtcaggc
    12421 ggagcggatc ctgtttgctt ttaatgatga cagagtcatt gtctatggct ttgactcaga
    12481 cagaaaagaa tgggacgcgc ttgatatgag tttacttccg aacgaaataa cgaaagaaaa
    12541 attactcaca gctgcgaagg atggaaaact ggatctgacg gtagatggtg aaacgctgga
    12601 aataaactta agtaagtgac aactctttta acggatacga cgctgtatct gaccaacaat
    12661 aatttgcgat ttatttcaca ggcctggtaa gcgcagcaca tcaggccatt acgtcgtaaa
    12721 atccggcaga gccctctctg ccggacatac tcattaatga acttgcggat ctgccggaga
    12781 cgcgttgttg cggatttcag caatatccat cgcattgaac agatagtggt taccgcagta
    12841 atcacaatgc atgtcaattt cgccatcttc cgccaggatg ctatcgactt cttcatcagg
    12901 cagcgttttc agcgcgtcgg cgcaacgttc acgcgagcag gtgcatttga actccacatc
    12961 ctgcggatcg taaaccgtca cctcttcttc gtgatacaaa cgccacaaca cttcgtttgc
    13021 cggtaaggtc agcagttctt cggttttgat ggtttcggtt agcgtcgcca ggtggtcaaa
    13081 gtcgtcctgc tgggcatttt gcgcaggcat tacctgcaac aacataccgc ctgcagccgg
    13141 tttgccgtct acgtcgccgg tgcgaataaa caggcgcgtc ggcagctgtt cagaacgcat
    13201 aaagtaatct tccaggcagg ccgccagggt atcaccttcc agaccaacca cgccctgata
    13261 gcgttcgcct tcgctcgggg taatggtgat caccacgtaa ccattgccga ccagcgtttt
    13321 caggtcggca ttttctggaa tttcgccctg cacgcgcgcc acaccgcgca tttgctggtt
    13381 attgttaccg ttaataaccg ccagattcat cggaccgtcg ccttgcagct gtacggtgat
    13441 atcaccatca aacttcagcg tagcggttaa caggctggtc gcaaccagca gttctgccag
    13501 cacgttttta acgggctgcg gataatcgtg attctcaagg atctgttgca gggtttccga
    13561 aacggttacc agttcgccgc gcacggcaaa gttttcaaac agatagcgat gtaattggtc
    13621 atgttgcggc ataatcatct ctcttgcagg tgacagttat tcactgtcgc cgtgtttaaa
    13681 tcgtaacagg tcgcggcgct cttttttgtc cgggcgtcgg tccgggtgcg gcatggttaa
    13741 ggcattaagt ttacgtgcca gcgccatttt ttcgcgtttc tctacacttt ccgcagtctc
    13801 ttcatacagc aaggctgcct cgctggcggg gcgacgctgt tcagtaatcg cctttacaat
    13861 caccgtgcgt tcgtcatttc cctggcgcag agtgagcgtg gcattcagct cgacgatttt
    13921 gctcggcttg ctgcgctgcc cgttgtaatg caccttaccg ccttcaatca tttcacgggc
    13981 cagcgcgcgg gttttataaa aacgggcagc ccatagccat ttatccagtc gaacctcaac
    14041 agcaggtttt tctttcatgg cgtctccttc acattagcga ggggatcagg cggcggtagt
    14101 cattcagtga cggatggcgt tgatactgtt tctcggcaat cccggaatca ggattagtca
    14161 cgccgaggca gtaacgaata ccaaattgcg cggcagcatc gagaatcgct tcgctgtcat
    14221 caataaacag cgttctttca gctttcagac ccgtagcttc ggccaccgca tgccataacc
    14281 gctgatcctc tttcggataa ccaaatgtgt gggtggaaag taataaatca aggtgtgcgt
    14341 ccagaccggt atgctcaagt tttaccgcca ggttgtgcgg atgcgcattg gtgagcaaaa
    14401 ttcgctgctt accgctggct ttcagtgcct caagaaacgg aatggtatct tcacgcagta
    14461 cggcacgcgg tcccatctcg gtggtcatcg cacagatatc cagacccagt tgctcactcc
    14521 agtaatcaag acagtaccag tttagcgtat gctgtacgtc gtgatattgc tggcgcatat
    14581 attccatcgc ttcctgtggc gtaaccccgt ttttcgcgcc ccatgtttca ggcaccagct
    14641 tttgccagaa atagttatcg aaggcgaggt cgagcaacgt gccgtccata tccagcagaa
    14701 cggtatctac gtcctgccag gcaatgttga tatgcatgag ggaaatctcc agagtgaagc
    14761 aatttgcgcg acagggtagc ataacctgcc gcgcaaacgt gttattcgat aaggctttct
    14821 gaaggggtga tcagttgcgg gttcaggcag ctttcataat aggcctgaat cttcatcatg
    14881 cgagtgcgat gacgctggta acgacgccag gcctgtacgc cattgtaaat ggcgctaccc
    14941 agcatcgtca gtagcagcaa tgtggtgctg agatagcgcc acaggccgga acgatccggg
    15001 attggatgaa ggccaatatg ctgcgtacca ttggcgtcag tgaagatttt tgtgacgata
    15061 ccctcggcgt taaacggcgt atgcatcagc atttgtgcca gtttctggaa agcgttccac
    15121 tgttcttgcg gcgggtagtc gtaaagtgat gccgaaggcc agggctgatc aacaaaatcg
    15181 ctgccttcgt cgctgacaat caggaatccg ccgggggccg gactgtttag tgattgtgcc
    15241 gctcgcgccg tttcatgcgt gataaacggc gcggtggagg ttgccaccag gttatccagc
    15301 gattccgcac tcaccgggcg taataacaca ttcacgccat cgagcttccc ggcgttggcg
    15361 cgttttacca gcgcgtccca gtctttactg ttgccgagat tgaccagcgc atttttcagt
    15421 cgcacacagt catctttggc agaacataaa tccgctgtct tcagtacaat gtcgccaaaa
    15481 tcatcaagca ataccatgcc ggatttttga attgctgaac gtaatgaggc actgacgcga
    15541 gatccatctt ccggtttagg gtgcagctgg cgattaactg cttcagtcaa tgccgtcgct
    15601 ttgttgacca gttcagattc tggtaatggc aatgagcggg cgtcgttcca gatgatctgc
    15661 gagcagtcaa acggtaaaaa aggtgaattg gttttcgcgc tccaggttcc ggaagtgcga
    15721 atattacaca ttcccgtacc gctgatacgc aatgtatcgc ctacccgcac gccagcgtca
    15781 gccagttgtt ttacgctggt ggcttcaatg gtctgtgcgc ctttcatcca tgagagagtg
    15841 aatttcagcg gcatatccag cgggatccag aataacagca taaacagcac cagcagcgag
    15901 cccgcagcga taatcgtact gcgcagccag tgctgtaacg gaaagttttt tacttcatca
    15961 tgcagcgaaa gatatcgccc ctgacgcact acgtggcggt cgagatagat atcaatatcg
    16021 gtttgttgac cgagatcctg agcaatgtat ggctgccagt gtgcgggata gaccaggtcg
    16081 ataataccga gtgaaatatt gttgatctgt tcctgatcgt tttcgccaaa caatccccaa
    16141 cgacggggtg taccgcgcag acaatgaatt tcccgcaggg aggattttgc cgggggcgcg
    16201 aatagccccc acagacctgc gcccagcagc agtaatgcgc cgcctgccag ccacggaaca
    16261 aatacatccg gggtaatcag gcagaaaaag aacatcagga aggaggcgac gatcagcaac
    16321 gcttcacgca gcccgcgcgg acgactcagc gcatattctt catgcgtttc tttgcgaata
    16381 ttgagtagct cgatttgctc actctcttcc ccgcgaatcg acgcctgcgt ggacggaaca
    16441 ggttgcaagg catagccgcg cgtttcctgc atgtactcct gcagcgtatg accgttgagt
    16501 gaaataacca gcggcagcga atcggtataa atcagttcaa cggtattctc atcgttgatg
    16561 tattgttccc agaaaggggg caggtggact tctaccgaat cgaggtagta acgccattta
    16621 ttcggatcgt cggtagagat gccgtaacgc gtgatagcgt gtgtcagcat catgacgttg
    16681 ttgctttcgg cattcagcgc cagagagatc ggtgccgcgc tagctcccgt tgggccaggc
    16741 acctgtaata cctgcgtcag gctctcaaga taattttcaa cggcgctacg ttcttcaggt
    16801 gtgagtttac gcgtttgcgc atccgcgaag gcgttggtcc agggcagctg acgccgtctg
    16861 gatcgcactt ttatcagcca tcccgcaagt agtgagcagg ccagcaaagc agctaaaaaa
    16921 atcacaatgg tgctcatgct ttccccatct tacttagtct gtcaggcgtg gtcagtcgca
    16981 ccagacacaa tacatgattc tgcggttggc tatcggcaag cgtggagttt aacttgagca
    17041 tcattaagca catcacagta accgctgatc atagcaaacc ctctgaaggc gggatatcag
    17101 caaattcctg gagttatttc aacctattga cttttaattt gatttgagga cgattattta
    17161 ttaagttaca taaatgtaaa taacatgcaa ttcacattgc cgaaagcgtg tccgatatcg
    17221 cacaataacc gctgatttca cagcatagat cccacgatga gcaaatcatt acaaaaaccc
    17281 accattctga atgttgaaac tgtagcccat tcccgactgt ttaccgtcga gagcgtggat
    17341 ctggagttca gcaatggcgt gcggcgtgtt tatgaacgaa tgcgtccaac caaccgggaa
    17401 gcagtgatga ttgtgccgat tgtggacgat cacctgatcc tgatccgcga atacgcagtg
    17461 ggaactgaat cctacgaatt aggtttttcg aaaggattaa ttgatccggg tgaaagcgtc
    17521 tacgaagccg ctaaccgcga gctaaaagaa gaggttggat ttggtgcgaa cgatctgact
    17581 tttttgaaga agctcagcat ggcaccgtct tacttttcca gcaaaatgaa tatcgtggtc
    17641 gcgcaagatc tctacccgga atcgctggaa ggcgatgagc cagagccgct accacaggtg
    17701 cgctggccgc tggcgcatat gatggatttg ctggaagacc ctgacttcaa cgaagcgcgt
    17761 aatgtcagcg cgctgttcct cgtgcgcgaa tggttgaaag ggcaggggcg agtgtaaatg
    17821 cgtgaagttt gcctgatgca caacgattat caggcttatg gggttcctgc aatatattga
    17881 tttttcacga ttttgttagc cggataacgc gttcacccgc atccggcata aacaaatgca
    17941 ctttgtcagc caactgaaaa ggcgccgaag cgccttttta atcagaacaa ttcctgtgcc
    18001 tcaccattat cgataatggt tgttcccacc tcgtgcaccg cctgttgtgt cggctgcgta
    18061 ccttcgatga aatactcttc gcggctgttg ccaccattag ctaactgccc ggtgctgcga
    18121 tcgatattca ccgtcacaat acccggaggc ggcgtcagcg gctgctccgg cacaccttca
    18181 agaacggctt tcatataagc gtcccatgca ggctgggcac tcttggcacc gccttcgtaa
    18241 cctgagatct gatctttaat cgctccggaa gccgttgtat gaccgagatt acgacggtga
    18301 tcatcaaagc caatccagac cgaggtcaca acgcccggac cgtaacccga gaaccacgca
    18361 tctttcgaac tgttagtggt accggttttc ccgccgatat cgcgacgctg caaatcacga
    18421 cctgcacgcc agccggtacc ctgccagcct ggttcaccaa agatattggt gttcaaagca
    18481 ctcttaatca ggaatgccag cggagtgttg atgacgtgcg gtgcgtactc ctgcgcgcca
    18541 gtcttcgcca ctaacgcctg atttgcctgc tccagctgcg gcattggtac agaaacattc
    18601 tgctgctcgc gggagatagc gacatcttca acatcgttat tttccagcac gttcgatttc
    18661 tgcgtatcac cgtaaatcac cggaatatcg cattccgggc aggctacttt cggtttcgct
    18721 tcgaaaatca cgccgccctg atcgttttca attttgctga taaaccacgg gtccaccagg
    18781 aagccgccgt tcgccatgac cgcgtagccg cgcgccacct gcattggggt gaaggacgct
    18841 gaacccagcg ccagcgattc ggtgtggaca atgttttgcg ccgggaagcc gaagcgttgc
    18901 agatattctg cagcgtagtc gacgcccatc gcccgcattg cacgtaccat caccacgttt
    18961 ttcgactgac ccagcccctg acgtaagcga attggaccag catactgcgg tggtgagttc
    19021 ttcggctgcc agtcagaacc gacacctgca tcccagcgag aaattggcac atcgttcaac
    19081 atacttgcca gcgtcagacc tttatccatc gccgcggtgt agaggaacgg tttgatgttg
    19141 gaacccacct gacgcagtgc ctgggtggcg cggttaaact tgctctgatt gaaatcaaag
    19201 ccaccgacca gcgccataac ggcaccattt tgcggattga tcgacaccag cgccgagttc
    19261 acttccggca cttgtgccag ccaccatgca tcgccaacct gacgaaccca gatttgctga
    19321 cccgtttgca gaacatcggt cactttacgc ggcgtcggtc cctgctgagt atccgaacgg
    19381 taaggacgcg cccagcgaac gccgtccata ctcaatgcga cagtcgaccc gtccgccagc
    19441 attgccgtcg cttcctgagg attggcgctg gtgactgcgg caggcagcag cggaccatag
    19501 gtcggcagcg ccttcagcgt atcggtaatc ttgttgttat cccacgccga ctcgcccact
    19561 ttccacagca catttgccgg gccgcgatag ccgtggcgca tgtcgtagtc cagcacgtta
    19621 ttacgtaccg cctgctgtgc ggcctgctgc actttgcggg tgatggtggt gtaaatgcga
    19681 taaccgtctt cataggcact ttcgccataa cggttataca tctcctggcg caccatttcg
    19741 ctcaggtacg gcgcagagaa agcaatctcc ggcgcgtgat agttagcgtt aatcgcctca
    19801 gtgcgtgtct gatcgaactg ttgttgggtg atataccctt catccagcat ccgcgacagt
    19861 acgacgttac gccgcgcgac ggcacgatcc attgagtaga gcggattaaa ggtggaaggc
    19921 gctttcggca gcccggctat cacagccatt tcgttcagcg tcagttggtc gaccgtttta
    19981 ccgaaataga cttgtgccgc agcaccgaca ccataggcgc ggtaaccgag gtaaatcttg
    20041 ttcagataaa gctcgaggat ctcgtctttc gtcagcaact gttcaatgcg aatcgcgagg
    20101 aagacttcct taatcttacg catcagcgtg cgttctggac tgaggaagaa gtttctcgcc
    20161 agctgctggg taatggtact cgccccttgt gacgcgtggc cggagaacag cgccacactt
    20221 gccgcacgga agatccccac cgggtcaacg ccgtgatgct cgtagaagcg gctgtcttcc
    20281 gtcgcgataa aggctttcac catctccggc gggatttgat ccaacgtaac cggaatacga
    20341 cgtttctcac cgtattgagc aatcagctcg ccatcggcgc tgtaaatctg catcggaatt
    20401 tgcaggcgaa catcttttaa tgtcgccaca tccggtagtt gtggctcgat gtagcggtat
    20461 aggccataaa tcgagcctgc tcccagcaga atgcaacaga ctgcaaggat caaaaaatac
    20521 tttacgaact tcactggaaa tttcccattt agtttcattt gggcagttta taaacaaacg
    20581 tgcggtagta taaaggcaag ccagacgcat tgatataccc gtcagagtga cgggtgataa
    20641 ggagatcatc acaatggcat ttaagatctg gcaaattggt ttgcatttac aacagcaaga
    20701 agcagtagcg gttgcgatcg tgcggggcgc aaaagaatgc tttttgcaac gctggtggcg
    20761 gttgccgctg gagaacgaca ttatcaaaga tgggcggatt gttgatgcgc agcggctggc
    20821 taaaacgttg ttaccgtgga gtcgcgaact gccgcagcgt catcacatta tgttggcgtt
    20881 tcccgccagt cgcacattac agcggtcatt tccgcgcccg tcgatgtccc tcggtgagcg
    20941 ggagcaaacg gcctggctgt cagggacgat ggcccgcgag ctggatatgg atccggactc
    21001 cctgcgtttc gattacagcg aagactcact cagtcccgcc tataacgtga ctgccgcgca
    21061 aagcaaagag ctggcaacgc tactcactct ggcagaaagg ttgcgtgttc atgtgagcgc
    21121 gatcaccccg gatgccagtg cattacagcg attcctgcct tttttacctt ctcatcagca
    21181 atgtctggcc tggcgtgata acgaacagtg gctgtgggcg acacgctgtc gctgggggcg
    21241 caaactggcg gtggggatga ctagcgcgaa ggagctggcg gcagcgttat ccgttgatcc
    21301 cgaaagcgtc gcgatatgtg gcgaaggcgg atttgatccc tgggaggccg tttctgttcg
    21361 tcagccgccg ctaccgccgc caggtggaga ctttgccatc gcgctggggc tggcgcttgg
    21421 gaaggcgtac tgatgaaccc gccaattaat tttttgccct ggcgacagca acgccggacc
    21481 gcttttcttc gtttctggtt gctgatgttc gttgcgcctc tgctgctggc cgtcgggata
    21541 acgctaatac tgcgtctgac agacaacgcc gaagcacgcg taaacaccgt tttgcttcag
    21601 gcggaacaac aactcgcccg cagcttacag ataacgaaac cacgtttgct ggagcgccaa
    21661 caaattcgtg agcagcgttt gcaacggcag cgccagcgac aatttacccg cgactggcaa
    21721 tctgcgctgg aagcactggc ggcgctttta cccgagcacg cctagctgac aacgataagc
    21781 tggcagcagg gaacgctgga gatcaagggg ctgacaacaa gcattaccgc gttaaacgca
    21841 ctagaaacgt cactccgcca ggatgcgtct tttcatctca atcagcgggg agccacgcag
    21901 caggatgcgc agggacgctg gcaatttgag tatcagttaa caaggaaggt tagcgatgaa
    21961 catgttcttt gactggtggt tcgccacatc accccgcctc cgccagtttt gctgggcatt
    22021 ctggttgctg atgttagtta cgctcatttt tctgtcatcg acacaccatg aagagcgcga
    22081 cgcattaatt cgactacggg caagtcatca ccagcagtgg gccacactgt atcgcctggt
    22141 tgacaccact cccttcagcg aagaaaaaac gctgcccttt tcgccactgg attttcagtt
    22201 gcccggcgcg caactggttt cctggcatcc atccgcgcag ggaggcgagt tggcgttgaa
    22261 aacgctgtgg gaagcagtgc cgtcggcatt tacacggctg gcagagcgca acgtcagcgt
    22321 gagccgtttt tcattaagcg tggaaggtga tgatcttttg ttcacgctac aactggagac
    22381 gccgcatgag ggttaaacgc tggttgttgg caggtattgt attgtgcctt ttaaccggta
    22441 tgcgtgaccc ttttaaaccg ccggaagatc tatgccggat tagcgaactt agccagtggc
    22501 gctatcaggg gatggtaggg cgaggcgagc gcatcatcgg tgtaataaaa gacgggcaaa
    22561 agaaatggcg acgggtgcag caaaacgatg tgctggaaaa cggctggaca attttacagc
    22621 tgacgccaga cgtactaacg ctgggtaccg ggacaaactg cgaaccgcca caatggttgt
    22681 ggcaacggca aggagataca aatgaagcaa tggatagccg cactactgtt gatgctgata
    22741 cccggcgtac aggcggcaaa gccgcaaaaa gtgacgctga tggtggatga cgttccggta
    22801 gctcaggtgt tgcaggcgct ggctgaacag gagaagttga acctggtcgt gtcgccagac
    22861 gtcagcggta cggtgtcgtt acatctaaca gatgttccct ggaagcaggc actacaaact
    22921 gtagtgaaaa gcgccggact gataacgcgg caggaaggca acattctctc agtgcattcc
    22981 attgcctggc agaataacaa tatcgcccgc caggaggcgg agcaggcgcg ggcgcaggca
    23041 aatctgccgc tggaaaatcg cagtataacc ctgcaatacg ccgacgcggg agaactggcg
    23101 aaagcggggg agaagctact gagtgccaaa gggagtatga ccgtcgataa acgcaccaat
    23161 cgccttttgc tacgagataa caaaacggcg ttaagcgcgc ttgaacagtg ggtagcgcaa
    23221 atggatctgc cggtcgggca ggttgagctg tcggcgcata ttgtcaccat taatgaaaaa
    23281 agtttgcgtg agttaggcgt gaaatggacg ctggccgatg cgcaacacgc tggtggcgtt
    23341 gggcaagtca ccacgcttgg tagcgacctc tccgtagcga cggcgacaac gcatgtcggt
    23401 tttaacattg ggcgcatcaa cggacgcttg ctggatcttg agctttccgc gctcgaacaa
    23461 aaacagcagc tggatattat cgccagtccg cgtctgctgg cctcacatct tcagcctgcc
    23521 agcattaaac aggggagcga aattccatat caggtttcca gcggggaaag tggcgcgacg
    23581 tcggtggaat ttaaagaggc cgtcctgggg atggaagtta cgcccacagt gttacaaaaa
    23641 ggtcgcatcc ggctgaaatt acacatcagc cagaacgttc cggggcaggt gctacagcag
    23701 gccgatggcg aagtgctggc gattgataag caggagatcg aaacgcaggt cgaggtcaaa
    23761 agcggagaaa cgttggcgct gggcggcatt tttacccgta aaaataaatc gggtcaggat
    23821 agcgtaccgt tgcttggcga cattccctgg ttcgggcaat tatttcgtca tgacggaaaa
    23881 gaagatgaac gacgcgagtt agtggtgttt atcacgccac gactggtttc cagtgagtaa
    23941 acagccgtaa aagcggtaat gtttttacgc tgaacgtgtt tcatctattt gacgcgcgca
    24001 ggtatttagc atacaaggag taccgatttg agagttggtg ctcttcgctg cctgcgttcc
    24061 atgatgatga tttatcattc aggcggcatt ttgctgtctt ttttacgcta atcttacccg
    24121 gtgatttatc gccagagcgg tggtagcaag gcagcgcgct tgcagcgacc agatatgcag
    24181 agggatgggt gatttattca gttgccaaac ccgctggagt attgagataa ttttcagtct
    24241 gactctcgca atatcttatg aggtttcagt tcatgtcctg cggcgctctc tgagcgaagc
    24301 gggtttatca ttaacgaata gtcttagtag taccgaaaaa atggcagaga aacgcaatat
    24361 ctttctggtt gggcctatgg gtgccggaaa aagcactatt gggcgccagt tagctcaaca
    24421 actcaatatg gaattttacg attccgatca agagattgag aaacgaaccg gagctgatgt
    24481 gggctgggtt ttcgatttag aaggcgaaga aggcttccgc gatcgcgaag aaaaggtcat
    24541 caatgagttg accgagaaac agggtattgt gctggctact ggcggtggct ctgtgaaatc
    24601 ccgtgaaacg cgtaaccgtc tttccgctcg tggcgttgtc gtttatcttg aaacgaccat
    24661 cgaaaagcaa cttgcacgca cgcagcgtga taaaaaacgc ccgttgctgc acgttgaaac
    24721 accgccacgt gaagttctgg aagcgttggc caatgaacgc aatccgctgt atgaagagat
    24781 tgccgacgtg accattcgta ctgatgatca aagcgctaaa gtggttgcaa accagattat
    24841 tcacatgctg gaaagcaact aattctggct ttatatacac tcgtctgcgg gtacagtaat
    24901 taaggtggat gtcgcgttat ggagaggatt gttgttactc tcggggaacg tagttaccca
    24961 attaccatcg catctggttt gtttaatgaa ccagcttcat tcttaccgct gaaatcgggc
    25021 gagcaggtca tgttggtcac caacgaaacc ctggctcctc tgtatctcga taaggtccgc
    25081 ggcgtacttg aacaggcggg tgttaatgtc gatagcgtta tcctccctga cggcgagcag
    25141 tataaaagcc tggctgtact cgataccgtc tttacggcgt tgttacaaaa gccgcatggt
    25201 cgcgatacta cgctggtggc gcttggcggc ggcgtagtgg gcgatctgac cggcttcgcg
    25261 gcggcgagtt atcagcgcgg tgttcgtttc attcaagtcc cgacgacgtt actgtcgcag
    25321 gtcgattcct ccgttggcgg caaaactgcg gtcaaccatc ccctcggtaa aaacatgatt
    25381 ggcgcgttct accagcctgc ttcagtggtg gtggatctcg actgtctgaa aacgcttccc
    25441 ccgcgtgagt tagcgtcggg gctggcagaa gtcatcaaat acggcattat tcttgacggt
    25501 gcgtttttta actggctgga agagaatctg gatgcgttgt tgcgtctgga cggtccggca
    25561 atggcgtact gtattcgccg ttgttgtgaa ctgaaggcag aagttgtcgc cgccgacgag
    25621 cgcgaaaccg ggttacgtgc tttactgaat ctgggacaca cctttggtca tgccattgaa
    25681 gctgaaatgg ggtatggcaa ttggttacat ggtgaagcgg tcgctgcggg tatggtgatg
    25741 gcggcgcgga cgtcggaacg tctcgggcag tttagttctg ccgaaacgca gcgtattata
    25801 accctgctca agcgggctgg gttaccggtc aatgggccgc gcgaaatgtc cgcgcaggcg
    25861 tatttaccgc atatgctgcg tgacaagaaa gtccttgcgg gagagatacg cttaattctt
    25921 ccgttggcaa ttggtaagag tgaagttcgc agcggcgttt cgcacgagct tgttcttaac
    25981 gccattgccg attgtcaatc agcgtaacaa caagaaaggt caggccgctt atcaagcggt
    26041 ctattagctt caggttaatt gcaacgtggt aagcattaac cttttagtgg ggtgttaaat
    26101 ggatgaattc aaaccagaag acgagctgaa acccgatccc agcgatcgtc gtactggtcg
    26161 ttctcgtcaa tcttctgaac gttctgagcg tactgaacgt ggcgaaccgc agatcaattt
    26221 tgatgatatt gaacttgatg acactgacga tcgccgtccg actcgtgcgc aaaaagagcg
    26281 caatgaggaa ccggaaatcg aagaagaaat tgacgaatcc gaagatgaaa ccgtggatga
    26341 agagcgcgta gagcgtcgtc cgcgtaagcg caaaaaagca gccagtaaac ccgcttctcg
    26401 tcagtatatg atgatgggcg tcggcattct ggttctactg ctgttgatca tcggtatcgg
    26461 ttctgcgcta aaagccccct cgacctcttc cagcgatcaa accgcgtctg gcgagaagag
    26521 tattgatctt gcaggcaatg cgaccgatca ggcgaatggg gtgcagccag cgccgggaac
    26581 cacgtctgcg gaaaatactc agcaggatgt ttctctgcca ccgatctctt ctacgccgac
    26641 tcaagggcaa accccggcgg caacggatgg tcaacaacgt gttgaagtgc agggtgacct
    26701 gaacaatgcg ctgacccagc cacaaaatca gcaacagttg aacaatgtgg cggtcaattc
    26761 cacattgccg accgaacccg cgactgtcgc gcctgttcgc aatggcaatg catcgcgtga
    26821 cacggcgaaa acgcaaaccg ctgaacgtcc gtccactacg cgcccagctc gtcagcaggc
    26881 ggtgattgaa ccgaaaaaac cgcaagcaac cgtgaaaacg gagccgaagc cggtagcaca
    26941 gacgccgaag cgtactgaac cagctgcccc tgtggcgagc acgaaggcac cgactgcgac
    27001 ttctacgcca gcaccaaaag agacggcgac tacggctcca gtacagacgg catccccggc
    27061 gcaaaccacg gcaacaccag ccgctggagg gaagaccgca ggtaatgttg gttcgttgaa
    27121 atcggcaccg tccagccatt acactctgca gctgagcagt tcctctaact acgacaacct
    27181 gaacggttgg gcgaagaaag agaatctgaa aaactacgtt gtctatgaaa cggcgcgtaa
    27241 tggtcagccg tggtatgtcc tggtttctgg cgtgtacgct tcgaaagaag aggcgaaaaa
    27301 agcggtatct acattgccag cagatgttca ggccaaaaac ccgtgggcga aaccgctgcg
    27361 tcaggtacag gccgatctga agtaatcaag gttatctccc gcaatggttt atcgttgcgg
    27421 gagctgcctg aagcgctgga tgctgtcgga gctttctcca cagccggaga aggtgtaatt
    27481 agttagtcag catgaagaaa aatcgcgctt ttttgaagtg ggcagggggc aagtatcccc
    27541 tgcttgatga tattaaacgg catttgccca agggcgaatg tctggttgag ccttttgtag
    27601 gtgccgggtc ggtgtttctc aacaccgact tttctcgtta tatccttgcc gatatcaata
    27661 gcgacctgat cagtctctat aacattgtga agatgcgtac tgatgagtac gtacaggccg
    27721 cacgcgagct gtttgttccc gaaacaaatt gcgccgaggt ttactatcag ttccgcgaag
    27781 agttcaacaa aagccaggat ccgttccgtc gggcggtact gtttttatat ttgaaccgct
    27841 acggttacaa cggcctgtgt cgttacaatc tgcgcggtga gtttaacgtg ccgtttggcc
    27901 gctacaaaaa aacctatttc ccggaagcag agttgtatca cttcgctgaa aaagcgcaga
    27961 atgccttttt ctattgtgag tcttacgccg atagcatggc gcgcgcagat gatgcatccg
    28021 tcgtctattg cgatccgcct tatgcaccgc tgtctgcgac cgccaacttt acggcgtatc
    28081 acacaaacag ttttacgctt gaacaacaag cgcatctggc ggagatcgcc gaaggtctgg
    28141 ttgagcgcca tattccagtg ctgatctcca atcacgatac gatgttaacg cgtgagtggt
    28201 atcagcgcgc aaaattgcat gtcgtcaaag ttcgacgcag tataagcagc aacggcggca
    28261 cacgtaaaaa ggtggacgaa ctgctggctt tgtacaaacc aggagtcgtt tcacccgcga
    28321 aaaaataatt ctcaaggaga agcggatgaa acagtatttg attgccccct caattctgtc
    28381 ggctgatttt gcccgcctgg gtgaagatac cgcaaaagcc ctggcagctg gcgctgatgt
    28441 cgtgcatttt gacgtcatgg ataaccacta tgttcccaat ctgacgattg ggccaatggt
    28501 gctgaaatcc ttgcgtaact atggcattac cgcccctatc gacgtacatc tgatggtgaa
    28561 acccgtcgat cgcattgtgc ctgatttcgc tgccgctggt gccagcatca ttacctttca
    28621 tccagaagcc tccgagcatg ttgaccgcac gctgcaactg attaaagaaa atggctgtaa
    28681 agcgggtctg gtatttaacc cggcgacacc tctgagctat ctggattacg tgatggataa
    28741 gctggatgtg atcctgctga tgtccgtcaa tcctggtttc ggcggtcagt ctttcattcc
    28801 tcaaacactg gataaactgc gcgaagtacg tcgccgtatc gacgagtctg gctttgacat
    28861 tcgtctggaa gtggacggtg gcgtgaaggt gaacaacatt ggcgaaatcg ctgcggcggg
    28921 cgcggatatg ttcgtcgccg gttcggcaat cttcgaccag ccagactaca aaaaagtcat
    28981 tgatgaaatg cgcagtgaac tggcaaaggt aagtcatgaa taagtttgaa gatattcgcg
    29041 gcgtcgcttt tgatcttgat ggtacgctgg tcgacagtgc tcccggtctt gctgctgcgg
    29101 tagatatggc gctgtatgcg ctggagttgc ccatcgcagg tgaagaacgc gttattacct
    29161 ggattggtaa cggcgcagat gttctgatgg agcgcgcatt gacctgggcg cgtcaggaac
    29221 gtgcgactca gcgtaaaaca atgggtaaac cgcccgttga tgacgacatt ccggcagaag
    29281 aacaggtacg tattctgcgt aaactgttcg atcgctacta tggcgaggtt gccgaagagg
    29341 ggacgttttt gttcccgcac gttgccgata cgttgggcgc gttgcaggct aaaggcctgc
    29401 cgctaggcct ggtcaccaac aaaccgacgc cgttcgtcgc accgctgctc gaagccttag
    29461 atattgccaa atactttagc gtggttatcg gcggcgatga tgtgcaaaac aaaaaaccgc
    29521 atccggaccc gctgttactg gtggctgagc ggatgggaat tgccccacaa cagatgctgt
    29581 ttgtcggcga ctcacgcaat gatattcagg cggcaaaagc ggcaggttgc ccatcagttg
    29641 gcttaaccta cggatataac tacggcgagg ctatcgatct cagccagcct gatgtaattt
    29701 atcagtctat aaatgacctt ctgcccgcat tagggcttcc gcatagcgaa aatcaggaat
    29761 cgaaaaatga ctaagcccat cgtttttagt ggcgcacagc cctcaggtga attgaccatt
    29821 ggtaactaca tgggtgcgct gcgtcagtgg gtaaacatgc aggatgacta ccattgcatt
    29881 tactgtatcg ttgaccaaca cgcgatcacc gtgcgccagg atgcacagaa gctgcgtaaa
    29941 gcgacgctgg atacgctggc cttgtatctg gcttgtggta tcgatcctga gaaaagcacc
    30001 atttttgttc agtcccacgt accagaacat gcgcagttag gctgggcact gaactgttat
    30061 acctacttcg gcgaactgag tcgcatgacg cagtttaaag ataaatctgc gcgttatgcc
    30121 gagaacatca acgctggtct gtttgactat ccggtgctga tggctgcgga catcctgctg
    30181 tatcaaacta atctggtacc ggtgggtgaa gaccagaaac agcacctgga actgagtcgc
    30241 gatatcgccc agcgtttcaa cgcgctgtat ggcgagatct ttaaggtgcc ggagccgttt
    30301 attccgaaat ctggcgcgcg cgtaatgtcg ctgctggagc cgaccaagaa gatgtccaag
    30361 tctgacgata accgcaataa cgttatcggc ctgctggaag atccgaaatc ggtagtgaag
    30421 aaaatcaaac gcgcggtcac tgactccgac gagccgccgg tagttcgcta cgatgtgcag
    30481 aacaaagcgg gcgtttccaa cctgctggat atcctttctg cggtaacggg ccagagcatc
    30541 ccagaactgg aaaaacagtt cgaaggcaag atgtatggtc atctgaaagg tgaagtggct
    30601 gatgccgttt ccggtatgct gactgaattg caggaacgct atcaccgttt ccgcaacgat
    30661 gaagccttcc tgcaacaggt gatgaaagac ggcgcagaaa aagccagtgt acacgcttcc
    30721 cgtacgctga aagcggtgta cgaagcgatt ggttttgtgg cgaagccgta atttaacatt
    30781 aatgccaaaa accggggaaa cccggttttt ttatcctcat ttgcaataat ccgaaaaaat
    30841 gtgaagcgcc tcgccgtttc cacatctcgt cgttgccact tatcttcatt ttaatgaaga
    30901 tataaatatt cattttattg aaaatatatt atgcgtcgaa cgtttatcaa aaaagaaggc
    30961 gtcgtcatca cgacgctggc ccgttatttg ttgggtgaaa aatgcggtaa tcgactgaaa
    31021 accatagatg agctggcaac tgaatgccgt tcatcggttg gcctgacgca ggccgcgttg
    31081 aaaacgttgg aatcaagcgg agcgatacgg attgaacgcc gtgggcgcaa tggcagttat
    31141 ctggtcgaga tggataacaa agcattgctg agtcatgtgg atatcaacaa cgtggtatgt
    31201 gcgatgcctt tgccctatac ccgcttgtac gaaggtttag cgagcgggct gaaagctcag
    31261 tttgatggca ttccttttta ctatgcgcat atgcgcggcg cggatattcg cgtggagtgt
    31321 ttacttaacg gcgtgtatga catggcggtg gtttcccgac tggcggcgga aagttatctc
    31381 acgcaaaaag ggttatgcct cgcgctggag ttggggccgc acacctacgt tggcgagcac
    31441 cagttgattt gccgtaaagg cgagtccgca aacgtgaagc gcgtggggct ggataaccgt
    31501 tcggcggatc agaaaatcat gaccgatgtc ttttttggcg atagtgatgt ggaacgtgtc
    31561 gatctctctt atcacgagag tttacaacgc attgttaaag gcgatgtcga tgcggttatc
    31621 tggaacgtgg tggcggaaaa cgaactgacc atgctgggat tagaggcgac gccgctcaca
    31681 gacgatccgc gatttttaca ggccaccgaa gccgtggtcc tgacgcgagt cgatgattac
    31741 ccaatgcaac aactgctgcg tgccgttgta gataaacacg ccctgctcgc ccatcaacaa
    31801 cgggtagtga gtggggaaca ggaaccgagt tattaaacga aaggcatctg atatggaaac
    31861 cagactcaac ctgctttgcg aggcaggcgt tattgataag gacatctgca aaggcatgat
    31921 gcaggtcgtc aacgtactgg aaacagagtg ccatctgccg gtgcgcagtg agcaaggaac
    31981 gatggcgatg acacatatgg cgagtgcact gatgcgcagt cgccgtggtg aagaaataga
    32041 gccgctggat aacgaattgc tggcagagct ggcgcaatcc agccactggc aagccgttgt
    32101 gcaattgcat caggtgttgt tgaaggaatt cgcactggaa gttaacccgt gtgaagaagg
    32161 ctatttgctg gccaaccttt atggattatg gatggctgct aacgaagagg tttgaagctc
    32221 ccgacaaatc cacgtcgcac aacctaaata ttcaaaaaaa tgaatattta attcaaggaa
    32281 cagaaacgag gcaacaagat gtttgtagaa gcattgaaac gccagaaccc ggcgctgatt
    32341 tccgccgcac taagtttgtg gcagcagggc aagatcgccc cggacagctg ggtgatcgac
    32401 gtggatcagg tactggaaaa cggtaagcga ctgattgaga cggcgcggct ttacggcatt
    32461 gaactgtatc tgatgaccaa gcagtttggc cgtaacccat ggctggcgga aaaattgctg
    32521 gcattaggtt acagcggcat tgtggcggtg gattacaaag aggcgcgagt catgcgccgc
    32581 gctggtttgt cggtggcaca tcaggggcat ctggtacaaa tcccttgtca tcaggttgct
    32641 gacgccgttg aacagggcac cgacgtcatc accgtgttta ctctcgacaa agcgcgggaa
    32701 gtttctgcgg cagcggtgaa ggccggacgt gtgcagtttg tgctgctcaa agtttatagc
    32761 gacgatgatt ttctttatcc ggggcaggag agcggttttg tacagcattc gctgcacgag
    32821 gtggttgctg aaattaagaa actgccgggg ctgcatttag ccggacttac ccatttccct
    32881 tgcctgcttt gggatgaggc tgccggaaaa gttttgccga caccgaatct tcacacgctg
    32941 atacaggcac gggatcaact ggcgaaatct ggtattgcac ttgagcaact gaacgcgcct
    33001 tcagcgacca gctgcacttc gctgccatta ctggcgtaat acggtgtgac tcatgccgaa
    33061 cccggtcatg cgctgacggg cactattccg gcaaaccagc agggcgatca gtctgaacgt
    33121 atcgcgatgc tctggttgag tgaaatctcc catcatttcc gtggcgacag ctactgctac
    33181 ggcggcggtt actaccgtcg tggtcatgcg caacatgcgt tggtgtttac gccagaaaat
    33241 caaaagatta ctgaaaccaa tctcaaaact gtggatgaca gcagtatcga ctacaccctg
    33301 tcgctggcag gcgagtttcc ggtaagcagt gcagtggtgc tctgtttccg cacgcagatt
    33361 tttgtcaccc gtagcgatgt ggtgctggtg tccggtattc accgtggcga accgaaaatc
    33421 gttggtcgtt atgacagtct tggaaactct ctgggggcgt aatggcgcga tttgtggtgt
    33481 tagtgattga tagctttggc gtaggggcaa tgaaagatgt cacgctggtg cgtccgcaag
    33541 atgcgggagc gaatacatgt ggtcacatcc tgagccagtt gccgcatttg cagctaccaa
    33601 cgctggagaa gctggggcta atcaacgcat tgggttatgc gccaggcgat atgcagccgt
    33661 cagattccgc aacctggggc gtggcagagc tgcaacatga aggtggcgat acctttatgg
    33721 ggcatcagga aattttaggc acgcgcccgt taccgccgct gcggatgcct tttcgcgatg
    33781 tgattgaccg tgttgagcag gcattagtat ccgccggttg gcaggtggag cgccgtggcg
    33841 atgaactgta atttctgtgg gtcaatcagg cggttgcgat tggcgataat ctcgaggcgg
    33901 atttaggcca agtctataac attaccgcca atctctctgt gatctctttt gacgacgcaa
    33961 tcaaaattgg tcgtatcgtg cgtgagcagg tacaggtcgg tcgggtcatt acatttggtg
    34021 gcctgttaac cgacagtcaa cgcattctcg atgccgcaga aagcaaagaa gggcgcttta
    34081 ttggtatcaa tgcgccgcgt tctggcgctt atgacaacgg cttccaggtc gtgcatatgg
    34141 gctacggtgt cgataaaaaa gtgcaggtgc cgcaaaaact gtatgaggcg ggcgtgccaa
    34201 ccgtgctggt gggtaaggtg gcagatatcg tcagcaatcc ttatggcgtg agctggcaaa
    34261 acctggtgga tagccagcgg attatggata tcaccctcga cgaatttaac acccatccga
    34321 cggcgtttat ttgcaccaat atccaggaaa ccgacctcgc tggtcatgca gaagacgtcg
    34381 cacgttatgc cgaacgtttg caggtcgttg accgtaacct tgcccggctt gttgatgcga
    34441 tgcagccaga tgattgcctg gtcgtgatgg cggatcacgg caacgatccg accattggtc
    34501 acagccacca tacccgcgaa gtggtgccgc tgctggttta tcagcaaggc ctggctgcta
    34561 cgcagctcgg tgtgcgcacc acgctttctg atgtgggagc taccgtgtgt gaatttttcc
    34621 gtgcgccacc gccacaaaat ggtcgctctt ttctttcctc cctccggttt gcaggagaca
    34681 ccctatgagt tttgatccga cgggttacac cctggcccat gagcatctgc atattgatct
    34741 ctccggcttt aaaaacaacg tggactgccg ccttgatcag tatgcgttca tttgccagga
    34801 gatgaacgac ctgatgaccc ggggtgtacg taatgtgatt gagatgacca accgttacat
    34861 ggggcgcaat gcgcaattta tgctcggtgt aatgcgcgaa accgggatca acgtggtggc
    34921 ctgtactggt tattaccagg atgcgttttt cccggaacat gtggtgaccc gcagcgtgca
    34981 ggaactggcg caggagatgg tcgatgaaat tgaacagggt atcgatggca cggagctgaa
    35041 agccgggatc atcgcggaga tcggctccag cgaaggaaag attacgccgc tggaagagaa
    35101 ggtatttatt gccgctgcgc tggcgcataa ccagaccgga cgcccgatct ccacgcatac
    35161 gtcgttcagc accatggggc tggaacaact ggctctgttg caagcccacg gagttgatct
    35221 ttcgcgcgtc accgttggtc actgcgatct gaaagacaac ctcgacaaca ttttgaagat
    35281 gatcgatctc ggcgcgtacg tgcagttcga caccattggt aagaacagtt actacccgga
    35341 cgaaaagcgc attgcgatgc tccacgcgct acgtgaccgt gggttgctga accgcgtcat
    35401 gctgtcgatg gatattacgc gccgctccca tttaaaagcc aatggtggtt atggctatga
    35461 ctatttattg accaccttta ttccgcaatt gcgccagtca ggattcagtc aggccgatgt
    35521 ggatgtgatg ttacgtgaaa ctccctctca atttttccaa taaggacaga ctcatgaaaa
    35581 agattggcgt tgcaggctta cagcgtgagc agattaaaaa aaccattgaa gcgacggcta
    35641 ctggcagttt tgaagttttc attcacaacg acatggaagc ggcaatgaaa gtgaaatccg
    35701 ggcaactgga ttactacatc ggtgcgtgta ataccggtgc gggcgcggca ttgtcgattg
    35761 ccatcgcggt gattggctat aacaaaagtt gcaccattgc caaaccaggc attaaagcga
    35821 aagacgagca tatcgccaaa atgatcgctg aaggaaaagt ggcgtttggc ctttccgttg
    35881 agcacgtcga acacgcgatt ccgatgctga ttaaccatct gaaataaaag gcacgactat
    35941 ggatctgtat attcagatta tcgtggtggc gtgcctgacg ggtatgacat cgcttctggc
    36001 gcatcgctcg gcggctgttt ttcatgacgg tatccgcccg atcctgccgc aactgattga
    36061 aggctatatg aaccgtcgcg aggcggggag tatcgctttt ggtctgagca ttggttttgt
    36121 ggcctcggtg gggatctctt ttaccctgaa aaccgggctg ctcaacgcat ggttactctt
    36181 tcttcctacc gatatcctcg gcgtactggc gataaacagc ctgatggcgt ttggtcttgg
    36241 cgctatctgg ggcgtgttga tccttacttg cctgttgcca gtaaaccagc tgctgaccgc
    36301 gctgccggtg gatgtattag gtagcctcgg ggaattaagc tcgccggtgg tttctgcttt
    36361 tgcactgttc ccgctggtgg cgattttcta ccagtttggc tggaagcaaa gtctggtcgc
    36421 cgccgtggtt gttctgatga cccgtgtggt agtcgtgcgc tatttcccac atcttaaccc
    36481 tgaatccatc gaaatcttta ttggcatggt gatgctgctg gggatcgcga taactcacga
    36541 cctgcgtcat cgtgatgaaa atgatattga tgccagcggg ctttcggtgt ttgaagaacg
    36601 cacgtcgcgg attatcaaaa acttacccta tatcgccatc gtgggagcat tgattgccgc
    36661 cgttgccagc atgaagattt tcgccggcag tgaagtgtcg atcttcactc tggagaaagc
    36721 gtactccgca ggcgtaacgc cggaacaatc gcaaacgctg atcaaccaag cggcactggc
    36781 agaatttatg cgcgggctgg gttttgtacc gttgattgcc accaccgcct tagcaacggg
    36841 tgtgtatgca gttgcgggct ttacctttgt ttatgcggtg ggctatctct cgccgaatcc
    36901 gatagttgca gcggtattag gcgcagtggt tatttcggcg gaagtcttgc tgcttcgttc
    36961 gatcggcaaa tggctgggac gctacccgtc ggtgcgtaat gcgtcggata acatccgtaa
    37021 cgccatgaat atgctgatgg aagtggcgct gctggtcggt tcgattttcg cagcaattaa
    37081 gatggcgggt tataccggat tctctatcgc ggttgccatt tacttcctca acgaatccct
    37141 gggccgtccg gtacagaaaa tggcggcacc ggtcgtggca gtaatgatca ccggtattct
    37201 gctgaatgtt ctttactggc ttggcctgtt cgttccggct taaggaaggc acctatgaag
    37261 acgtttcctc tgcaaagtct gacgattatt gaggcgcagc aaaagcagtt tgcgctggtg
    37321 gatagcattt gtcgccattt ccccggcagc gagtttctta ctggcggtga tttaggctta
    37381 acgccaggac tgaatcaacc gcgcgttacc cagcgtgtgg aacaggtgct ggctgatgca
    37441 tttcacgcac aggctgcggc gctggtgcag ggtgcgggta ctggcgcgat tcgtgcggcg
    37501 ttggcggctt tgctcaaacc ggggcagcgt cttctggtac atgatgcgcc tgtttacccg
    37561 acgacacagg tcattattga gcagatgggg ctgacgctta ttactgctga tttcaatgac
    37621 ctgtcggcac tgaagcaggt cgtcgacgag caactaccgg atgcggcgct ggtgcagcat
    37681 acgcgccagc agccgcagga cggctacatt ctggcagatg tgctggcaac attgcgctcg
    37741 gctggcgttc ctgcattaac cgatgacaac tatgcggtga tgaaagtggc gcgaattggc
    37801 tgcgaatgcg gggcaaatgt ctcgactttt tcctgcttca agctgtttgg gccggagggt
    37861 gttggcgcgg tggtaggcga tgccgatgtt attagtcgga ttcgctccac gctctattcc
    37921 ggcggcagcc agattcaggg cgctcaggcg ctggaagtct tgtgtgggct ggtgtttgcg
    37981 ccagtgatgc acgcggtgca ggcgggggta tctgaacggt tgctggcttt gcttaacggt
    38041 ggtgcggtgc cggaagtgaa aagcgcggtg attgctaatg cgcagtcgaa ggtgttgatt
    38101 gtcgagtttc atcagccgat tgccgccaga gtgctggaag aggcgcaaaa gcgcggtgcc
    38161 ttgccttacc cggtgggtgc agagtcgaaa tatgaaatcc cgccgctctt ttatcgcctt
    38221 tccggaacgt ttcgccaggc gaatccacaa tcagaacatt gtgcgattcg cattaacccg
    38281 aatcgcaacg gtgaagagac ggtgctgcgg attttgcgtg agagtattgc cagtatttaa
    38341 tgctggatag caccgtcaag ctaaattccg tactgaacgg tcccctcgcc cctttgggga
    38401 gagggttagg gtgaggggaa cgtacgggat gtaaaattca acgcgccagc atcgccggat
    38461 gcgatgctgg cgcatcttat ccggcctacg gttaatgtct tttattatca atcgttaagg
    38521 tgatgcgatt ggcgcggcag aagagttcgg agacgtgcac cggtttgtca ttctgatcaa
    38581 aggcgatttt gctgatgcga aacaacggtt cgcccagttc gcatttcagc cattttgcct
    38641 gcgggcgggt ggcggcaaag atatcgatgg tctttttgtc gctgaccact cgcgtgtcaa
    38701 aacgctcctg aaataactga taggtggagc ttccttcgac gtaaatctca tcaaagtcag
    38761 gataacgcga cagcgggatc catgaactat caataaacag cggctcttta tcgagataca
    38821 tcacccggca gagatggaac acttcgctgt ttccggggat gttcagcttt tcgcaaaacg
    38881 gcgcggcgct gacccgttcc tgttcgatca ctttctcttt cgtcgccttg ccttgtgaga
    38941 cgccaaaatc ggtaaaacca ctgacagtaa gcagggcgtt ttcaactttc tggctttgta
    39001 caaaggtgcc tttgccctgc cagcggatca gtacgccgtc tgccactaag tcgctgatag
    39061 ctttgcgaat agtaatgcgg ctgacgttat attgcgtaca aagctcgttt tcggtaggga
    39121 tctgttgccc ggcctggtaa accccctgcg cgatatcatc cagcagccgc tggcggacgg
    39181 tagcgtagag gagttgatga gagtagcggt ccgtagctga cataatcgac cctgaaataa
    39241 agaatgaatt acacggatta tagggtcgtt ttccggcaag taacagcacg ttggcgcgga
    39301 gatgctcacg ccaacgttat acctaccagg caccgtgata ctgaatggtt ttcgccgcgc
    39361 acgccgttcc ctgcgccatc gcctgcggta atgtcatccc cgcagaccag ccgcaaagga
    39421 atccggcaat gaacgaatct ccggcaccca tggtgtcgat aaccgtcacc ggttcaggag
    39481 cctgacgcca gaactgcgcg ccatcccagg caatgctgcc gttttcaccc agcgtgacaa
    39541 tcactgttcc tgcgccacgg gcaacaatcg ctttcatctt cagacgcagc gcttcgtctt
    39601 cttgcggtgc ggaggcaaag gcaaaatcga gatgcggcac cagtgtctgc cagagcgggc
    39661 tgtcccactt gtcggagaag tcgaaagcgg taagtttgcc cgcagcgtgc agctgtggga
    39721 atgcgtcttc cgcatgtccc cagattgccg cgtgcacaat gtcatactgc gccagccagg
    39781 cgtaatcctc ttcactcagg gcaaagtcgg ccatcacgcc ttcggtgtag tcgccaaaaa
    39841 cgcgatcatt gtcgtgcagt tccacctgag tttgtgcggt aacgccgtgt ttcgtatgga
    39901 catggctgat atcgacgccc atgcgggcga gatcctgctt cagctttgtg ccgtagtcat
    39961 cgtcacccac caaggtaatg catcccggct gtatgccgta gcgagtgcag tacaccgcca
    40021 cattgaccgc attaccgcca gaaaacgctt tattcagttg cgggtagata tcgacgcagt
    40081 tatcgccgat tgtcgccagg gttttcatct ctcatcctcc ggcagcagcg cgcgaaagcg
    40141 ttccagcgcc tggcgggcat agagtctggg ctcgttcata tacatcgtca ccagctccac
    40201 cgtacagtaa ccctcatagc cccgctcaat aatatcgcgc atcagttccc gcagcggcat
    40261 tttgccttct ccaggaatgt aatgcgtgtc gctggccccg tcgctgtcga caatatgcag
    40321 atgacgataa tttatcgccc agtttgtcga aataactcat caccggttcc gcctggacat
    40381 acggcgcgca aatgtcgacc atgctgaaca ggcgcggcga aggcaccagc gccagcgcat
    40441 gaagcacatc attagcatta cacacgacgt tcgattcata cggcgttaac ggttcgagga
    40501 ttagatccat gccgatgttt tccgcgtact cacacagttc gcttaggttc tctgccagcc
    40561 gtccccagat aacattaggt ggcgtgagat agcctgcgtg ggccgcggaa atcagcgtat
    40621 aacccgcgtt catctctttt gccatatcca tcgccagctt gatcatgtcg aggctttcgc
    40681 gacgcatatg ttcatcgccc agcatcatgt tatacggata gccgttggtt tctggcgtat
    40741 agccgataat cggcatctga tacgtctgcg ccagcgcctt gatttgtttg atgccgcccg
    40801 cttttaagtc cggcgcgaac gcgtgcgggc gaccgcccca aatttcgatg ccgtcgtaac
    40861 ccagctcgct tgcatcacga aatgcgtgtt caataggcag ccgctggtgg ccgcaggtaa
    40921 acatacctgt tttcatcggg taactccttg cgtaaatccg gcccgttgcc gggccgggag
    40981 agggattaat attccaccag accaccgtaa tagcggcgtt catccgggtt gtgatctttg
    41041 taaatagaca ggtagtagca gagccactcc attggcacga acatcaggaa cggtgccagc
    41101 cacgggtgca gcccttgcga aatttcggcg taatcgatga cgatcacgtt gtcagtacgc
    41161 tgtttaacaa agttaatggc gcgttcggtg gtgtggcgac tttcatcatt gccgagcagg
    41221 aacaggaacg gaacgcccgg ttcgacaatc tccagcgggc catggcggaa ctctccgctc
    41281 tcaatcacgc agccgtgcgt ccaggtaaat tccatcagcg ttacaatgcc ttctttgtaa
    41341 cccagcggac gcagcggacc cgcagcaacg gtataaatca tcggccactg gctggccagt
    41401 tcaccaagct ggcggccttt ttcttcccag gtgcgtacca gatgaccgag cgcattcggc
    41461 aacggcttga gatcgttttt gatcttgccg atttccgcgt tcggcgcgag gcgggtgatc
    41521 atctccagca ccacgctgta gcagagcagc aggtgaattt cccagataca gtcggcctga
    41581 taatcaatgc taaattccgc cgccgaggta atcgggctgt ccgcgcgttt ggtgaacgcc
    41641 gcagtgagtg cgccgcaggc ccggcccagc tccagcgctt tgattacctc ttccgtttta
    41701 ccgtagtcag aaacgccaat caccgcgcaa cgatcgtcga ggcgatacgg ggtgttatcg
    41761 cagaactccc agccggaaat ggcgtagacc tgaagatcgg aaaagcgatc cgccagatgt
    41821 ttcgccgttt gcgccgcgtt gagtggcgag ccgcaggcaa cgaaataaat acgatcaatg
    41881 tcgcgcttca ccatctcttc cacgatggcg tgcaccagcg gaacgtcatg gctgagaact
    41941 ttttccactt cctgaaccat attttcggtg accagaaagt ccacggtgct tttatcaata
    42001 tccaacattt tattttctcc agataacatt acgacaaagc gttgagctgg cggctacgct
    42061 tcgcccagaa ggcgtaagca ggcagaccag tagcaataac gatgacggcg cagataaggc
    42121 cgggaatcgg tgcccagaca aaggttgagg cgaccagaat gaggcttgac gcaatggcga
    42181 gggtggtcat cagcccgaaa gccggagtac gccacagcgg tttgtaatcg tcgcgtttac
    42241 gacaccagat gatggagccg aaggtgaggg tatttttgaa acacatcacc agggtgaaat
    42301 aacccagcag gctggtgaga tcggaaacga agatgaagaa gatccccagc gccccttgca
    42361 ggatgatgga gacatccggc gtgttgtatt tcggatgcac atggccgaag catttaaaga
    42421 acaggttgtc tttcgccatc gcgtattcca gccgcggctg gtacatcacg cagctggaaa
    42481 gcgaaccaag aatgacgatc atcgccgtga tggcaacaaa gatcccagcg gtgctgccga
    42541 gtgcggggat ccaggtcagg gcgtcggaaa tcggcgtttc agaattggcg agtttgtcga
    42601 agggcatcag gccggaaatc accagcgcca gcagggtgta gagcaccaga accagcagac
    42661 aggaaccaat cagcgctcgt ggcatggttt ttccggggtt tttaatttcg ccggtcatat
    42721 aacagataga ggccatgccg gtatacgacc aactggtggc agagatcccc gccagcagcg
    42781 ccataaagct gcccgttgcg ccaatcgcag tggtggtagg ggcggcaaaa ttctccgctt
    42841 taaaccagaa gatcccaagg ccaatgacga tagtgaacgg gataattttg gcgatggtaa
    42901 ttagcgtctg aaacgctgcg ccgccttcaa cggagcgcag gtgtagcaac ataaaggcga
    42961 taattaatcc ggcggcgata aatttaccga gcaacggatc gataggcgtt aaaaagccaa
    43021 gattgctgac aatcgccagc gccataatcg acaatgacgg cgcatcgttg gcccagaagc
    43081 tggcccagcc ggagaggaaa gccagcggtc ggcttccggc atttttcaga taaacataat
    43141 ctgcgccatt ttccggataa gcggtggata gttccgcata gacgcacatt tgcgggatca
    43201 caattaactc gccaatgaca aacgcgagca ccgtaagcca cggcgtgccc gctgcttttg
    43261 ccacttcacc cacagataca aaaataccgg agccgacggt tgtcccgacg gcgattgcaa
    43321 gaacggccca aaatccgagc ttgcgttgga gttcctggct tccca
//
LOCUS       DAJVQG010000014        41303 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJVQG010000014 DAJVQG010000000
VERSION     DAJVQG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41303
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.014"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(26..1156)
                     /gene="dnaJ"
                     /locus_tag="OQM83_000733"
     CDS             complement(26..1156)
                     /gene="dnaJ"
                     /locus_tag="OQM83_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCS1519069.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1245..3161)
                     /gene="dnaK"
                     /locus_tag="OQM83_000734"
     CDS             complement(1245..3161)
                     /gene="dnaK"
                     /locus_tag="OQM83_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCS1519070.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3538..3942
                     /locus_tag="OQM83_000735"
     CDS             3538..3942
                     /locus_tag="OQM83_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCS1519071.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            3968..4681
                     /gene="msyB"
                     /locus_tag="OQM83_000736"
     CDS             3968..4681
                     /gene="msyB"
                     /locus_tag="OQM83_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCS1519072.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            4830..5396
                     /gene="satP"
                     /locus_tag="OQM83_000737"
     CDS             4830..5396
                     /gene="satP"
                     /locus_tag="OQM83_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCS1519073.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5431..6018)
                     /gene="mog"
                     /locus_tag="OQM83_000738"
     CDS             complement(5431..6018)
                     /gene="mog"
                     /locus_tag="OQM83_000738"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCS1519074.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6133..7086)
                     /gene="tal"
                     /locus_tag="OQM83_000739"
     CDS             complement(6133..7086)
                     /gene="tal"
                     /locus_tag="OQM83_000739"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCS1519075.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7365..8794
                     /locus_tag="OQM83_000740"
                     /pseudo
     CDS             7365..8794
                     /locus_tag="OQM83_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            8864..9640
                     /gene="yaaA"
                     /locus_tag="OQM83_000741"
     CDS             8864..9640
                     /gene="yaaA"
                     /locus_tag="OQM83_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCS1519076.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(9793..10089)
                     /locus_tag="OQM83_000742"
     CDS             complement(9793..10089)
                     /locus_tag="OQM83_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCS1519077.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10303..11589)
                     /gene="thrC"
                     /locus_tag="OQM83_000743"
     CDS             complement(10303..11589)
                     /gene="thrC"
                     /locus_tag="OQM83_000743"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCS1519078.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(11590..12522)
                     /gene="thrB"
                     /locus_tag="OQM83_000744"
     CDS             complement(11590..12522)
                     /gene="thrB"
                     /locus_tag="OQM83_000744"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCS1519079.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12524..14986)
                     /gene="thrA"
                     /locus_tag="OQM83_000745"
     CDS             complement(12524..14986)
                     /gene="thrA"
                     /locus_tag="OQM83_000745"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCS1519080.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15013..15132)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15068..15133)
                     /gene="thrL"
                     /locus_tag="OQM83_000746"
     CDS             complement(15068..15133)
                     /gene="thrL"
                     /locus_tag="OQM83_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCS1519081.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15347..16033)
                     /locus_tag="OQM83_000747"
     CDS             complement(15347..16033)
                     /locus_tag="OQM83_000747"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCS1519082.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16433..16573)
                     /locus_tag="OQM83_000748"
     CDS             complement(16433..16573)
                     /locus_tag="OQM83_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1519083.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            16669..17385
                     /gene="arcA"
                     /locus_tag="OQM83_000749"
     CDS             16669..17385
                     /gene="arcA"
                     /locus_tag="OQM83_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCS1519084.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17445..18797)
                     /gene="creD"
                     /locus_tag="OQM83_000750"
     CDS             complement(17445..18797)
                     /gene="creD"
                     /locus_tag="OQM83_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCS1519085.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(18855..20279)
                     /gene="creC"
                     /locus_tag="OQM83_000751"
     CDS             complement(18855..20279)
                     /gene="creC"
                     /locus_tag="OQM83_000751"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCS1519086.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20279..20968)
                     /gene="creB"
                     /locus_tag="OQM83_000752"
     CDS             complement(20279..20968)
                     /gene="creB"
                     /locus_tag="OQM83_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCS1519087.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(20981..21454)
                     /gene="creA"
                     /locus_tag="OQM83_000753"
     CDS             complement(20981..21454)
                     /gene="creA"
                     /locus_tag="OQM83_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCS1519088.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            21665..22534
                     /gene="robA"
                     /locus_tag="OQM83_000754"
     CDS             21665..22534
                     /gene="robA"
                     /locus_tag="OQM83_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCS1519089.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(22531..23178)
                     /gene="gpmB"
                     /locus_tag="OQM83_000755"
     CDS             complement(22531..23178)
                     /gene="gpmB"
                     /locus_tag="OQM83_000755"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCS1519090.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23230..23751
                     /gene="yjjX"
                     /locus_tag="OQM83_000756"
     CDS             23230..23751
                     /gene="yjjX"
                     /locus_tag="OQM83_000756"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCS1519091.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(23836..24162)
                     /gene="trpR"
                     /locus_tag="OQM83_000757"
     CDS             complement(23836..24162)
                     /gene="trpR"
                     /locus_tag="OQM83_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCS1519092.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24252..26189)
                     /gene="sltY"
                     /locus_tag="OQM83_000758"
     CDS             complement(24252..26189)
                     /gene="sltY"
                     /locus_tag="OQM83_000758"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCS1519093.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            26400..28067
                     /gene="ettA"
                     /locus_tag="OQM83_000759"
     CDS             26400..28067
                     /gene="ettA"
                     /locus_tag="OQM83_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCS1519094.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28374..29606)
                     /gene="nadR"
                     /locus_tag="OQM83_000760"
     CDS             complement(28374..29606)
                     /gene="nadR"
                     /locus_tag="OQM83_000760"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCS1519095.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(29627..31009)
                     /gene="radA"
                     /locus_tag="OQM83_000761"
     CDS             complement(29627..31009)
                     /gene="radA"
                     /locus_tag="OQM83_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCS1519096.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31058..32026)
                     /gene="serB"
                     /locus_tag="OQM83_000762"
     CDS             complement(31058..32026)
                     /gene="serB"
                     /locus_tag="OQM83_000762"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCS1519097.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32132..32776
                     /locus_tag="OQM83_000763"
     CDS             32132..32776
                     /locus_tag="OQM83_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCS1519098.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            32804..33820
                     /gene="lplA"
                     /locus_tag="OQM83_000764"
     CDS             32804..33820
                     /gene="lplA"
                     /locus_tag="OQM83_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HCS1519099.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            33852..34001
                     /locus_tag="OQM83_000765"
     CDS             33852..34001
                     /locus_tag="OQM83_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1519100.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(34276..34995)
                     /gene="deoD"
                     /locus_tag="OQM83_000766"
     CDS             complement(34276..34995)
                     /gene="deoD"
                     /locus_tag="OQM83_000766"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCS1519101.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35075..36298)
                     /gene="deoB"
                     /locus_tag="OQM83_000767"
     CDS             complement(35075..36298)
                     /gene="deoB"
                     /locus_tag="OQM83_000767"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCS1519102.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(36350..37672)
                     /gene="deoA"
                     /locus_tag="OQM83_000768"
     CDS             complement(36350..37672)
                     /gene="deoA"
                     /locus_tag="OQM83_000768"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCS1519103.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(37799..38578)
                     /gene="deoC"
                     /locus_tag="OQM83_000769"
     CDS             complement(37799..38578)
                     /gene="deoC"
                     /locus_tag="OQM83_000769"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCS1519104.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            38836..40386
                     /locus_tag="OQM83_000770"
     CDS             38836..40386
                     /locus_tag="OQM83_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCS1519105.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGSSSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            40358..41221
                     /locus_tag="OQM83_000771"
     CDS             40358..41221
                     /locus_tag="OQM83_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCS1519106.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
BASE COUNT         9711 a        11223 c        10377 g         9992 t
ORIGIN      
        1 gcccacgggc aggcttttgg ggaggttagc gggtcaggtc gtcaaaaaac ttcttcacac
       61 catcaaagaa gctctttgag cgcgggctgt tgtgctcacc ggttgggcca ccgaagcttt
      121 cttgcagctc ttgcagcaac tgtttctgct tctcgttcag gcctaccggt gtttcgacga
      181 caacgcggca cagcaaatca ccctgtgcgc caccgcggac agacttgacg cctttaccgc
      241 gcatacggaa aagcttaccg gtctgggttt cgccaggcac tttcagtttg acgcgaccat
      301 caagggtcgg tacttcgatt tcgccaccca gcgccgccat agcgaagttg atcgggactt
      361 cgcaatagag gttgttgcct tcacgctcga aaatcgggtg ctgtttaacc tgaacctgaa
      421 cgtacagatc gcctgccggt gcgccatgct cgcccgcttc accttcgccc gcaagacgga
      481 tgcggtctcc agtgtccacc cctgccggga ttttaacgga cagcgttttg ctgcgctcaa
      541 cacgaccatg accatgacat ttgttgcacg gatctttgat cagcgtaccg cggccctgac
      601 agtgtggaca ggtctgctgt acagcgaaaa atccctggcg catctgcacc tgaccagaac
      661 catgacaggt cggacaggtc tgcggctgtg tacctggttt tgcaccgcta ccgtggcaaa
      721 cgtcacactc ttccagagtc ggaatgcgga tctctttggt cacgccacgt acagcttctt
      781 cgagggtgag ctccatgtta tagcgtaaat cagcaccgcg cgccgcacgt tgacgaccac
      841 gtccgccgcc aaaaatatcg ccgaaaacgt caccaaaaat atcgctgaag tctgcgccgc
      901 cgccaaaacc gccgccgccc atgccacctt gctcaaacgc agcatgacca tactgatcgt
      961 atgccgcacg tttttgcgag tcggtcagaa cttcataagc ttccttgatc tctttaaatt
     1021 tcgcctcggc ctctttgtca ccctggttac ggtccgggtg gtatttcatg gccaggcgtt
     1081 tgtaggcctt tttgatttca cgctcttccg ctgttttgga aacgcctaaa atctcgtaat
     1141 aatcttgctt agccatcttt tttaaattgc ccctagatga atgcacgggc ggagaggaaa
     1201 ttccccttcg cccgtgtcag tataattacc cgtttatagg gcgattattt tttgtctttg
     1261 acttcttcaa attcagcgtc gacaacatcg tcatctttcg cgttgtttgc agaagcatca
     1321 gcaccggcag tctgctgctg ggcatgttgc tgctgggcga tttccatcag tttctgggaa
     1381 acctgtgcca gttcctgcat tttcgcttcg atagcggctt tgtcttcacc tttcagagca
     1441 gtttccagtg cagtcagcgc agactcgata gcagttttgt cgtcagccgg cagtttgtcg
     1501 cctgcttctt caacctgctt acgggtgctg tgcagcagat ggtcgccctg gttgcgagtc
     1561 tgtaccagct cttcaaactt acggtcagct tcggcgttag cttctgcgtc gcgtaccatt
     1621 ttctggattt catcttcgtt cagaccagaa gaagccttga tggtgatctt ctgctcttta
     1681 ccgctgtttt tatctttcgc ggaaacgtgc aagataccgt cagcatcgat atcgaaggta
     1741 acttcgatct gcggcatgcc gcgcggtgcc gggttgatac catccaggtt gaactgaccc
     1801 agagatttgt tatcagctgc acgtttacgt tcaccctgca gcacatggat ggttaccgca
     1861 gactggttgt cttcagcggt agagaacacc tggctgtgct tggtcgggat agtggtattt
     1921 ttcgcgatca gcgtcgtcat cacaccgccc atggtttcga tacccagaga cagcggggta
     1981 acgtccagca gcagtacgtc ttttacgtca ccagtcagaa caccaccctg aacagcagca
     2041 ccgattgcta cagcttcgtc cgggttaacg tctttacgcg gctctttacc aaagaactca
     2101 gcaactttct tctgaaccat tggcatacga gtctgaccac ccacgaggat aacgtcgtcg
     2161 atatcagata cggacaggcc agcgtcctgc agtgcaactt tcagcggctc aatggaacgg
     2221 tttaccagat cttcaaccag gctttccagt ttcgcacgag tcactttgat gttcatgtgt
     2281 ttcggaccgg tcgcatctgc agtgatgtac ggcaggttaa cgtcggtctg ctgagcggaa
     2341 gacagttcga ttttcgcttt ttctgccgct tctttcaggc gctgcattgc cagcggatcg
     2401 ttgcgcaggt caatgccctg atctttcttg aattcttcaa ccaggtagtt gatcagacgg
     2461 ctgtcgaagt cttcaccccc caggtgggta tcaccgttgg tagccagaac ttcgaaggtt
     2521 ttttcgccgt caacttcgtc gatttcgata atagaaatat cgaaagtacc accacccagg
     2581 tcataaaccg cgatagtacg gttgccagta cctttgtcca gaccgtaagc cagcgcagct
     2641 gcggtcggtt cgttgatgat acgttttact tccagaccag cgatacggcc tgcgtctttg
     2701 gttgcctgac gctgagcatc gttaaagtat gccggtacgg tgataacagc ttcagttacc
     2761 ggttcaccca agtaatcttc agcggttttc ttcatttttt tcagcacttc agcagaaatc
     2821 tgcggcggtg ccattttctg gcctttaact tcgacccatg cgtcgccgtt atcagcagca
     2881 ataattttga acggcatgat ggaaacatca cgctgtactt cttcgtcctg gaagcggcgg
     2941 ccaatcaggc gtttaatcgc aaacagggtg ttttgcgggt tcgtcactgc ctgacgttta
     3001 gccggctgac caaccagagt ttcaccatcc tgggtatagg caataataga aggcgtggtg
     3061 cgatcgcctt cggcgttctc cagcacgcga ggagtggtgc catccataat cgctacacaa
     3121 gagttggtag tacccaggtc gataccaatt attttaccca tctaaacgtc tccactatat
     3181 attcggtcat catgtggttg tgagtctgta ataggggcga aacgtctggt ttcaactgcc
     3241 caatttcatt tttttgcaga ctcactcact gcggttgact actaaatggg gtcgtaaccc
     3301 acgtcatcaa gggggagatg caaaaatttt ttgtgctttt gcgcagaaat ttcgcgatat
     3361 ggcaggacaa gacgtaaaat tgacagcaat tttattcata taaagaatga atcgcagcca
     3421 aggcattcat caaaaaattg taataaaaag aaaagattac gtacctgaat cttctcttta
     3481 tcagcagtaa actagtgggt attcatccct ctacctcttc ccactaagag aatccttatg
     3541 aaatccgttt ttacgatttc cgccagcctg gcgattagcc tgatgctgtg ctgcacggcg
     3601 caggcaaacg accataaact cctcggcgtc attgcaatgc cgcgtaacga aaccaacgat
     3661 ctggcgctga aacttcctgt ttgtcgcatt gtgaaacgca tacaactctc tgccgaccat
     3721 ggcgatttac agttaagcgg tgcatcggtt tatttcaaag ccgcccgtag cgccagtcag
     3781 agtctgaata ttccttcaga aataaaagaa gggcaaacca ctgactggat caacattaac
     3841 agtgataacg acaataaacg ctgcgtctca aaaatcacct tttccggtca tacggtgaac
     3901 tcatcggata tggccacgct gaaaattatc ggcgacgact aacgccagaa tatagaaacc
     3961 acaaaaaatg aatgttaatt acctgaatga ttcagatctg gattttcttc agcattgtag
     4021 tgaggaacag ttggcaaatt tcgcccgatt gctcacccat aatgaaaaag gcaaaactcg
     4081 cctctccagc gtactgatgc gcaacgaact gtttaaatcg atggaagggc atcccgagca
     4141 acatcgccgc aactggcagc tgattgctgg agaattacag cattttggtg gcgatagtat
     4201 cgccaacaaa ctgcgcggac acggtaaatt gtatcgggcc attttgctcg atgtttcaaa
     4261 acgattgaag ctgaaagccg acaaagagat gtctacgttt gagattgagc aacagttact
     4321 ggaacaattt ctgcgtaaca cctggaagaa tatggacgag gaacataagc aggagtttct
     4381 gcacgcggtc gatgccaggg tgaatgagct ggaagagctg ctgccgctgc tgatgaaaga
     4441 caaattattg gcaaaaggcg tgtcgcattt gctttccagc caattgaccc gcattttacg
     4501 cacccacgca gcaatgagcg tgcttgggca tggtttgctg cgcggcgcgg gtctgggagg
     4561 ccctgtgggc gcagcattaa atggggttaa agcggtcagc ggcagtgcct atcgcgtgac
     4621 aattccagcc gtaatacaaa tcgcctgcct gcgccggatg gttagcgcca ctcaggtctg
     4681 acaccgtaaa ccggggaaaa ttttcatttt cccctcaaaa gatcgtagac actgccccac
     4741 tggctgatta ttatgccgcg ccctgaaaac actactgtta ttcagggaaa ttatttcacc
     4801 attcattcga tgatgatttt tgaggaatta tgggcaacac taagttggct aatccggcac
     4861 cgctgggcct gatgggcttc ggcatgacca ccattctgct taacctgcac aacgtgggtt
     4921 atttcgctct ggacggtatt attcttgcca tgggcatttt ctacggcggc atcgcgcaaa
     4981 tttttgccgg tctgctggag tacaaaaaag gcaacacttt cggtttaacc gcattcacct
     5041 cttacggttc tttctggttg acgctggttg cgattctgct gatgccgaaa ctgggtctga
     5101 ccgatgcgcc aaatgcacag ttccttggtg tctacctggg tctgtggggc gtatttacgc
     5161 tgtttatgtt cttcggcacg ctgaaaggcg cacgcgttct gcaattcgtt ttctttagcc
     5221 tgactgtgct gtttgccctg ctggcgatcg gtaacattgc cggtaacgcc gcaatcatcc
     5281 actttgccgg ctggattggt ctgatctgtg gtgccagtgc aatctatctg gcgatgggtg
     5341 aagtactgaa cgagcagttt ggtcgcaccg ttctgccgat tggtgaatcc cactaattgt
     5401 tttgagatcc ccccgctcgg ggggattttt ttattcgcta acgtcgcgtc ttgcactctt
     5461 cggtctgaat gcagcaacca cttctggtgc cgtttcaacg tatggccctt ccagcaactg
     5521 aatgcagtac ggtacgctgg caaaaatacc gtgtaccaca acgttacccg cagcgtcctt
     5581 cacaccttcc agcgtctctt taatagactt cggctgaccg ggtaagttaa ggatcagcgc
     5641 ctgtttgcga atcacaccaa cctgacgcga aaggatcgca gttggtacaa aatgcaggct
     5701 tatctggcgc atctgttcac caaagccagg catctcgcgg tccgctactg ccagcgtcgc
     5761 atcgggcgtt acgtcacgac gcgccgggcc agttccgccc gtggtgagca ccagatggca
     5821 actcatttca tccactagct cacacaacgt ttgctcgatg atcgcctgct catcggggat
     5881 taagcgggtt tccagttcaa acggcgtggt tagcgccgat gtcagccatt cttccagcgc
     5941 agggatgcct ttatcctgat aaacgccgct ggatgcgcga tcagagatgg aaactaagcc
     6001 aatacgtaaa gtattcatca agatttttcc gtttaaacag tctcgttaaa cagaatgata
     6061 cactgcgaag ggagtgacag acaggcttca gaagaggtag cgtgaccgac ttcccggtca
     6121 cgctaagaat gattacagca gatcgccgat cattttttcc agtttttcct ggtcaatagc
     6181 aaacttacgg ataccttccg ccagtttatc tactgccatt ggatcctggt tgtgctgcca
     6241 caggaactcg gactcagtga tacgcgccgg acgcgctttc acttcgccgg tgtaagacag
     6301 tttacgttcg atagcccctt cgctctcagc cagctctttc agcagtgccg gtgcgatggt
     6361 aagacggtcg cagcctgcca gttccagaat ttcgccgatg ttacggaagc ttgcgcccat
     6421 aaccacggtt tcataaccgt gctctttgta gtactggtag atttcagata cagaaaccac
     6481 gcccggatct tctgccggag cgtactcttt cttatcagta ttcgctttgt accagtcaag
     6541 aatacggcca acaaatggcg agatcaggaa cacgcctgct tccgcacaag cacgagcctg
     6601 agcgaaggag aacagcaggg tcaggttaca gttgatgcct tctttttcca gctgttctgc
     6661 agcacggata ccctgccaag tagaagccag tttgatcaga atacgatcgt tgctaatacc
     6721 agcatcgttg tagagtttga tcaggcgttt tgctttcgca attgacgctt cggtgtcata
     6781 ggaaagacgc gcatcaactt cagttgagat acggcccgga accagtttca ggatttccag
     6841 accaatattt actgccagtt tgtcggtcgc gtccacgatc tgctgcgcgc gatcgttgct
     6901 ctgctgtttc gcccaggcga cagcatcatc aatcaactta cggtattccg gaatctgcgc
     6961 tgcgttaaga atgagagaag ggttggttgt ggcatcctgc ggttgataca gcttcattgc
     7021 cgcgatgtcc ccagtgtcgg ccactacggt ggtgtactga cgaagggagg tcaatttgtc
     7081 cgtcatgata gtatttctct ttaaacagct tgttaggggg atgtaaccgg tctgccctga
     7141 tgatatcacg acgcaacgtc agcgcaaccg ccgacaagac ggttatcgcc aggcgagact
     7201 gtttcggatt tctgacactg cgttgatatc gcccgccatt tttatacaaa acctcatgta
     7261 tgctacgcag aagttatcaa gtacctcgta gcgtatatac ttcttaaaca attggtaacg
     7321 tttacacagg aaagtcatcg cgaccggcaa taagagggat atgcatgcca gattttttct
     7381 cctttattaa cagcgtcctt tggggatcgg taatgattta cctgctcttc ggcgcaggtt
     7441 gttggttcac ttttcgcacc ggatttgtgc agtttcgcta catccgccag tttggcaaaa
     7501 gtcttaaaaa tagcattcat ccacagccag gcggtttaac ctcatttcag tcattgtgta
     7561 ccagtcttgc ggcgcgcgtg ggtagcggca acctggccgg cgttgcgctg gctattaccg
     7621 ccggtggacc tggagccgtc ttttggatgt gggttgccgc gtttatcggc atggcgacct
     7681 cgtttgccga atgttccctt gcacaacttt ataaagaacg tgacgtcaat ggacagtttc
     7741 gtggaggacc ggcatggtat atggcgcgcg ggctggggat gcgttggatg ggcgttctgt
     7801 tcgccgtctt tttgctcatc gcctacggca taattttcag cggagttcag gcgaacgccg
     7861 ttgcccgagc cctgagtttt cttttgattt tcccccgctg gtgacaggca ttattctcgc
     7921 tgtctttgct ctgctggcaa tcactcgcgg tcttcatggc gtcgcccggc tcatgcaggg
     7981 gtttgtcccg ttgatggcga taatctgggt gctgaccagc atggtgattt gcgtaataaa
     8041 tatcgggcaa cttccccacg tcatttggtc tatttttgag agtgcttttg gctggcagga
     8101 agcggcaggc ggcgcggcgg gatatacctt aagccaggcg attactaacg gttttcagcg
     8161 cagtatgttt tccaatgagg cgggaatggg ttcgacgcca aacgcggcag cggcagcggc
     8221 gtcctggcct ccgcatccgg cagcgcaagg gattgtccag atgattggca tttttatcga
     8281 caccctggtc atctgtacgg caagcgccat gctgatatta ctggcgggta acgacacaac
     8341 ctacatgccg ctggaaggta ttcagcttat ccagaaggcg atgcgggtgc taatgggttc
     8401 ctggggtgct gagtttgtta ccctcgtggt tattctgttt gccttcagct ccatcgttgc
     8461 caactacatt tatgccgaaa acaatctctt ctttttacgc ctgaacaacc ctaaagcgat
     8521 ctggtgtttg cggatctgca ccttcgcaac ggtcatcggc ggcaccttgc taagtcttcc
     8581 gctgatgtgg caactggcag atatcataat ggcctgcatg gctattacca atttgaccgc
     8641 cattttactg ctctcgcctg tggttcatac cattgccagt gattatctac gccagcgtaa
     8701 actcggcgtg cgcccggtgt ttgatccgtt gcgttatccg gatatcggtc gccagctttc
     8761 tccggacgcg tgggatgatg tttcgcagga gtaatcacaa ctatcgatca actcattctc
     8821 tttttttgct aaagtcggca taaatttcct gcaaggactg gatatgctga ttcttatttc
     8881 acctgcgaaa acgcttgatt accaaagccc gttgaccacc acgcgctata cgctgccgga
     8941 gctgttagac aattcccagc acttgatcca tgaagcgcgg aaactgacgc ctccgcagat
     9001 tagcacgctg atgcgcatca gcgacaaact ggcgggtatc aacgccgctc gctttcatga
     9061 ctggcagcca gatttcacgc cggagaatgc ccgccaggct attttggcgt ttaaaggtga
     9121 tgtctacacc ggcttgcagg ccgaaacttt cagcgaagac gatttcgatt ttgcccaaca
     9181 gcatttgcga atgctttccg gcttgtatgg cgtactgcgc ccgctcgatt taatgcagcc
     9241 ttatcgtctg gaaatgggga tccgtcttga gaatgcccga gggaaagatc tgtatcaatt
     9301 ctggggagat atcatcacca acaagctgaa cgaggcgctc gcagcacaag gcgataatgt
     9361 ggtgattaat ctggcgtcag atgagtattt taaatcggtg aagccgaaga aattgaatgc
     9421 cgagattatc aagccggtgt tcctcgatga gaagaacggc aaatttaaga taatcagctt
     9481 ctacgctaag aaagcacgcg gtctgatgag tcgtttcatt attgaaaatc ggctgaccaa
     9541 accagagcaa ctgactggtt ttaatagcga aggttacttc tttgatgaag cttcctccag
     9601 caatggcgaa ctggtgttta aacgctacga gcagcgttaa gtttgcagtc aatgccggat
     9661 gcggcgtgaa cgccttatcc ggcctacaaa agcatgcaga ttcaatatat tgcagaattc
     9721 acgtaggcct gataagacgc gccagcgtcg catcaggcgc tgaatgccgg attgttaccc
     9781 ggcatttgtc atttagcggt gatgtttgcc tggaccatga ccgccgtgat gatcatgagg
     9841 agctttctta tggtggcgcg gcggtggcgg cggtccgtgt gggtgccagc gattgcctcg
     9901 ccattcataa tgttgtttcc accagccgtg gtcgcgccag tgacctccat cccagtaata
     9961 gccacgatta tcacgatcgc ctatctgtaa ttttactgac gggactaacg taatttccgc
    10021 agcctgtgct gccatgggag cgaccagaac cagggaaagt gcgagtacga tagattgcat
    10081 ctttttcaca ggttactcct tttatcattg cccgttatgg gtcgatatcc ggagattacg
    10141 cgatgagaac gacggttgtt attctccgca atcctaatct ctaagttacc gcatttattg
    10201 agaatttctc ctttttccct gtcgtttttc tccataattt cttcataaaa aagccgggct
    10261 gcataaaagc aaacccggcc tgattgagat aatgaataga ttttactgat gattcatcat
    10321 caatttacgc aacgcagcaa aatcggcggg cagattatgt gaaagcaagg gtaaatcagc
    10381 acgttctgcc agctcttttg gcagatccaa cgtttcaccg agaatcgctt ccacgctctc
    10441 tttaaatttc gccggatgcg cggtgccgag gaacaagcca tattcgcctg gattcaactg
    10501 gtcacacagc gcacgatacg ctacggcagc gtgcggctcc gaggtgtagc ccagttcttt
    10561 taactcacgc attgtctgtt gcgtggtttc atcatccacg gctgcataac ccagctcttt
    10621 cagttgccag attttgcggc ggaacaactc ttccacacgc ggccagttgt tcggctgact
    10681 cacgtccatc gcgttggata acgtcgcctg agtcgctttg ggtgaccact gaccgtcgtg
    10741 caggaaacgt ggcacggtat cgttcacgtt ggtcgcagca ataaaacgtt tcaccggcag
    10801 accgagtgac ttcgccagca gacccgccgt caaatcgccg aagtttccgc ttggcaccga
    10861 gacaaccagc tggttgcgcg cttcctgcgg cagctgcgca actgcttcaa agtagtagca
    10921 aatctgcgcc agcaaacggc tgatgttaat cgagttagcc gagtttaacc ccagcgccac
    10981 tttcagttct tcatcatcaa acgcctgctt caccagcgcc tgacaggcat cgaaatcgcc
    11041 gtcgatggca acagtttcga tattgccgcc caacgtacag aacagttttt cttgcagtgg
    11101 actgattttg cctcgtggat agaggataac cactttcaca ttcggcaaac cgtagaaagc
    11161 atgagccact gccgctccgg tatcaccgga ggtcgcggtc agaatggtca ctggcttatc
    11221 gcccgcaata tgggtcagca tttgtgccat aaagcgaccg ccgaaatctt taaatgccag
    11281 cgttggcccg tggaacaatt ccagacaacc gacatcgctt tcaacattgg cgaccggagc
    11341 cgggaaggca aacgccgcgc gcacgcgctc ttccaggatt tcctgcggga tttcatcacc
    11401 aataaacgcc gagaggatct tcgcactgcg ggtgacaaaa tccagcttca gcatctcatc
    11461 aatttcagtc aggctgaatt ccggcaggtc atgcggaaaa aacagcccct gatttttgcc
    11521 caacccctgg gttacggctt gcgcaaagct gacctgctcg ttgtgatctt tcagattgta
    11581 gagtttcatt tagttttcca gtactcgtgc gcccgccgta tccagccggc aaatatgaac
    11641 aaaaccttcc tgattttgca ggtagttctt acccaaccag tcggcaacgc gctgggcggt
    11701 atccggcttg tcacatagag cgaacaaggt cgggccggag ccggagatac cgctcgctac
    11761 cgcgccgatt tccgcgaccg cctgccgcgc ctgccggaag ccaggcagta accgttcacg
    11821 gtagggttca gcgataacat ctttcatcag cttcgcggca agctcaggct gacgggaata
    11881 gcaggcgtga atgaagccag ccagatgtcg cccgtgcgca atgcaatcct ggcggcgata
    11941 ctgcgccggt aaaatagccc gggcttctgc cgtcgagact ttaattcccg gatacgccag
    12001 cacccacagc cactcatcaa accctggcac ttgctggctg atgatgtcgt tttcttcgat
    12061 catcaactgc ataccaccaa gaaaacacgg tgccacgttg tcgtaatgaa tgctgccgga
    12121 gatacgtcct tccagctcgc ccatcaaagc cagcaaacgg gtgtcattaa gtggcttgcc
    12181 gcagtgttca ttcatcgcca tcagcgccgc gaccaccgaa caggcgctgg agcctaagcc
    12241 cgagccgatc ggcatattct tttccagagt catcgccact ggaatttgct tgcccagctc
    12301 caggcaaaaa cgctcccagc actgataaac gatattttcc cgtggttctg acggcagctt
    12361 atcggcaaag cgtccgaggt tgttgagact gaatgtctct gccgcctcaa ccgtgactac
    12421 atctccgagc aatgcaccat caacgggtgt caccgccgcc ccgagcacat caaacccgac
    12481 gctcatattg gcactggaag ccggggcata aactttaacc atgtcagact cctaacttcc
    12541 atgagagggt acgtagcaga tcggcaaaga caccagcagc ggtaacgtca ttgcccgcac
    12601 catatccgcg cagcaccaac ggcagcggct gataatagtg gctataaaag gccagggcgt
    12661 tttcgccatt tttcactttg aacagcggat cattcccatc cacttcggca atcttcacgc
    12721 ggcagacacc atcttcatca atattgccaa catagcgcaa aacttttcct tcatcacggg
    12781 ccttcgccac gcgcgcggca aagagatcgt cgagctgtga cagattcgcc ataaaagcgg
    12841 caacatcacc ctcggcgtta aactctgcgg gcagcacagg ttcaatttca atatccgcca
    12901 gttccagttc acgtcccgtt tcacgagcga gaatcaatag tttacgcgcc acatccatac
    12961 cagaaagatc atctcgcggg tccggttcgg tataacccat ttcccgcgcc agcgtggtcg
    13021 cctcggagaa actcatgcct tcgtctaact tgccgaagat ataagaaagc gaacctgaaa
    13081 gaatgccgga gaacttcacc aattcatcac ctgcattgag cagattttgc aggttctcaa
    13141 taaccggtaa tccagcccca acgttggtgt catagaggaa tttacgccgc gatttttccg
    13201 ccgcataacg caactgatga tagtaatcca tcgacgaggt gttggccttt ttgttcggcg
    13261 tgacaacgtg gaaaccttcg cgcaggaagt cggcatattg atccgccact gcctggctgg
    13321 aggtgcagtc aacaatgacc gggttcagca gatgatattc tttcacgagg cgaattaagc
    13381 gcccgagatt aaacggctct ttggcttgtg caagttcttc ctgccagttt tccagattaa
    13441 ggccatgcac actagtgagc agagccttcg agttggcaac gccgcagaca cgtaagtcga
    13501 tatgtttatt cttcagccag ctttgctgac gcttcagttg ctccagcagc gcaccgccaa
    13561 cgccaccgac gccaatcaca aacacttcga taacctgatc ggtattgaac agcatctgat
    13621 gagtaacgcg cacgccagtg gtcgcatcat cgttatttac cacgacagag attgagcgtt
    13681 cagaagatcc ctgagcaatg gcgacaatgt tgatattggc gcgggccagc gcggcaaaga
    13741 atttcgccga gatcccacgc aaggtgcgca taccatcacc taccaccgag ataatggcca
    13801 gccgttccat caccgccagc ggctccagta agccttcttt cagttccagg tagaactctt
    13861 cctgcattgc ccgttcagcc cgcacacagt cgctttgcgg aacgcagaaa ctgatactgt
    13921 attcggaaga tgattgcgta atcagcacca cggaaatacg ggcgcgtgac atcgcggcaa
    13981 agacgcgcgc cgccatgccg accattcctt tcatccccgg gccggaaacg ctgaacattg
    14041 ccatgttatt cagattggaa atgcccttga ccggtaattc gccttcatca cgactggcac
    14101 caatgagcgt acctggtgct tgaggatttc cggtattttt aatcaggcaa gggatctgga
    14161 actgggcgat gggggtaatg gtgcgggggt gaagaacttt agcgccgaag taggaaagct
    14221 ccatcgcttc ctggtaggac atcgacttca acaacctcgc atcgggcacc tgacgcgggt
    14281 cgcaggtata gaccccgtca acgtccgtcc aaatctcgca acaatcggcg cgtaaacagg
    14341 cagccagcac cgccgcggag tagtcggaac cgttgcgtcc aagtaccacc agttcgcctt
    14401 tttcattacc ggcggtgaag cctgccatca gcaccatgtg atcagccgga atacgacttg
    14461 ccgcaatacg gcgggtggac tcagcaatat cgacggtaga ttcgaggtaa tgccccactg
    14521 ccagtagttt ttcgaccgga tcgataacgg taacgttgtg accacgcgct tctaacacgc
    14581 cggccataat ggcgatcgac attttctcgc cacggcaaat cagcgcagcg ttaatgctat
    14641 ccgggcactg ccctaacaaa ctaatgccat gcaggacatg ttttatttgg gcaaattcct
    14701 ggtcgacgaa agttttcaat tgcgccagcg ggaatcccgg ctgggcggcg gcgagtcccg
    14761 tcaaaagttc ggcaaaaata cgttcggcat cgctgatatt gggtaaagca tcctggccgc
    14821 taatggtttt ttcaatcatc gccaccaggt ggttggtgat tttggcgggg gcagagagga
    14881 cggtggccac ctgcccctgc ctggcattgc tttccagaat atcggcaacc cgcagaaaac
    14941 gttctgcatt tgccactgat gtaccgccga acttcaacac tcgcatggtt gttacctcgt
    15001 tacctttggt cgaaaaaaaa agcccgcact gtcaggtgcg ggcttttttc tgtgtttcct
    15061 gtacgcgtca gcccgcaccg ttacctgtgg taatggtgat ggtggtggta atggtggtgc
    15121 taatgcgttt catggatgtt gtgtactctg taatttttat ctgtctgtgc gctatgccta
    15181 tattggttaa agtatttagt gacctaagtc aataaaattt taatttactc atggcaggta
    15241 accagttcag aagctgctat cagacactct ttttttaatc cacacagaga catattgccc
    15301 gttgcagtca gaatgaaaag ctgaaaaata cttactaagg cgttttttat ttggtgatat
    15361 ttttttcaat atcatgcagc aaacggtgca acattgccgt gtctcgttgc tctaaaagcc
    15421 ccaggcgttg ctgtaaccag tcgaccagtt ttatgtcatc tgccactgcc agagtcgtca
    15481 gcaatgccat gactcgttcg cgtaaagctt gcagttgatg ttggtctgcc gttgtgtcac
    15541 ttttcgccgg ttgttgtatt aatgttgcta attgatagca atagaccatc accgcctgcc
    15601 ccagattgag cgaaggataa tccgccacca tcggcacacc agtaagaacg tcagccaacg
    15661 ctaactcttc gttagtcagc ccggaatctt cgcgaccaaa caccagcgcg gcatggctca
    15721 tccatgaaga tttttcctct aacagcggca ccagttctac tggcgtggcg tagtaatgat
    15781 atttcgcccg actgcgcgca gtggtggcga cagtgaaatc gacatcgtgt aacgattcag
    15841 ccagtgttgg gaaaacttta atattatcaa taacatcacc agatccatgt gcgacccagc
    15901 gggtggctgg ctccaggtgt gcctgactat cgacaatccg cagttcgcta aaacccatcg
    15961 ttttcattgc ccgcgccgct gccccaatat tttctgctct ggcgggtgcg accaggataa
    16021 tcgttatacg catattgcca ctcttcttga ccaaataacc gcgaacaggg tgatcactgt
    16081 caacttatta cgcggtgcga atttacaaat tcttaacgta agtcgcagaa aaagcccttt
    16141 acttagctta aaaaaggcta aactatttcc tgactgtact aacggtttaa ttgttaaaaa
    16201 atgctacata tccttctgtt tacttaggat aattttataa aaaataaatc tcgacaattg
    16261 gattcaccac gtttattagt tgtatgatgc aactagttgg attattaaaa taatgtgacg
    16321 aaagctagca tttagatacg atgatttcat caaactgtta acgtgctaca attgaacttg
    16381 atatatgtca acgaagcgta gttttattgg gtgtccggcc cctcttagcc tgttatgttg
    16441 ctgttaaaat ggttaggatg acagccgttt ttgacactgt cgggtcctga gggaaagtac
    16501 ccacgaccaa gctaatgatg ttgttgacgt tgatggaaag tgcatcaaga acgcaattac
    16561 gtactttagt catgttacgc cgatcatgtt aatttgcagc atgcatcagg caggtcaggg
    16621 acttttgtac ttcctgtttc gatttagttg gcaatttagg tagcaaacat gcagaccccg
    16681 cacattctta tcgttgaaga cgagttggta acacgcaaca cgttgaaaag tattttcgaa
    16741 gcggaaggct atgatgtttt cgaagcgaca gatggcgcgg aaatgcatca gatcctctct
    16801 gaatatgaca tcaacctggt gatcatggat atcaatctgc cgggtaagaa cggtcttctg
    16861 ttagcgcgtg aactgcgcga gcaggcgaat gttgcgttga tgttcctgac tggccgtgac
    16921 aacgaagtcg ataaaattct cggcctcgaa atcggtgcag atgactacat caccaaaccg
    16981 ttcaacccgc gtgaactgac gattcgtgca cgcaacctgc tgtcccgtac catgaatctg
    17041 ggtactgtca gcgaagaacg tcgtagcgtt gaaagctaca agttcaatgg ttgggaactg
    17101 gacatcaaca gccgttcgtt gatcggccct gatggcgagc agtacaagct gccgcgcagc
    17161 gagttccgcg ccatgcttca cttctgtgaa aacccaggca aaattcagtc ccgtgctgaa
    17221 ctgctgaaga aaatgaccgg ccgtgagctg aaaccacacg accgtactgt agacgtgacg
    17281 atccgccgta ttcgtaaaca tttcgaatct acgccggata cgccggaaat catcgccacc
    17341 atccacggtg aaggttatcg cttctgtggt gatctggaag attaatcggc tttaccaccg
    17401 tcaaaaaaaa cggcgctttt tagcgccgtt tttatttttc aaccttattt ccagatacgt
    17461 aattcatcgt ccgttgtaac ttctttactg gctttcattt tcggcagtga aaacgcatac
    17521 cagtcgatat tacgagtcac aaacatcatg ccggccagcg ccaccaccag cacactggtt
    17581 cccaacaaca gcgcgctatc gacagagttg agcagtcccc acatcactcc atccagcaac
    17641 aacagcgcga gggtaaacaa catgctattg cgccagcctt tcaataccgc ctgcaaataa
    17701 ataccgttca ttaacgcccc aatcagactg gcgattatcc atgccacggt aaaaccgata
    17761 tgttcagaaa gcgccagcaa gagcaaataa aacatcacca atgaaagccc caccagcaaa
    17821 tattgcattg ggtgtaaacg ttgcgcggtg agcgtttcaa aaacaaagaa cgccataaaa
    17881 gtcagtgcaa tcagcagaat ggcgtactta gtcgcccggt ccgttaattg gtattgatcg
    17941 gctggcgtcg tcactgcgac gctaaacgcg aggaagtttt cccagccggt atcattgcct
    18001 gaagcaaaac gctcaccgag attattagca aaccagctgc tttgccattg cgcctgaaaa
    18061 cctgactcgc taacttcccg tttggctggc aaaaaatcac ctaaaaaact gggatgcggc
    18121 cagttgctgg ttaaggtcat ttcgctatta cgcccggcag gcaccacaga aagatcgccg
    18181 gtaccgctta aattcagggc catattcagc ttcaggttct gcttccgcca gtccccttcg
    18241 ggtaaaggga tatgcacgcc ctgcccgcct tgctctaacc cggtgccggg ttcaatggtc
    18301 agcgccgttc cgttaacttc aggcgctttc accacaccaa taccacgcgc atccccgacg
    18361 ctaatcacaa taaatggctt gccaaaggtg atatttggcg cgtcgagttc gctaagacgc
    18421 gaaacatcga aatcggcttt tagcgttaaa tcactgtgcc agacctgacc ggtataaatc
    18481 cctatcttgc gttcttccac gttctgattg ccatcaacca tcaatgactc aggtaaccaa
    18541 aaatggataa aacttcgttt ccgctccacg gttttatcct cttcctgtac cgtataaagc
    18601 tcggtcacag gaatagcgat aagcggccca acaagttttt gcggcccgct ggtactttgg
    18661 cgaatcgcat cttccacatc gctacggtaa tcggcacgtt cgacaatcac ctgccgaatc
    18721 agcattatcg gaatcaacaa cagcaatact gcaccaaaca gggtggtcat tttccagaac
    18781 agggaggatt tcaacatagt cttctccttt gcaatggcag caggatacga aggctatgtg
    18841 ggaagaattt gaagctatgt gaagtgacgg tgaagtcgaa gcgaggccag cacgccacct
    18901 tcctgcacgt tgcgcagcgt gacttcgccg ttaaacaaac gggcgacctc actgacgaac
    18961 gccaacccca gaccgctgct tttttgccca tttgcacgcg gcaaagagta aaagcgttca
    19021 aaaatacgtg acagcgcgta gtcaggaata ccactaccgg tatccagcac cttaagcgtg
    19081 acgtgttcct gatccacttc ggcgcttagc gttatacgac cgctctcggg ggtaaaatcg
    19141 atggcgttat ccagtaaatt ccccagcgcc tgatccagta acgccggttc agcagcaaca
    19201 ttaacctcgg tgggcgtaac atgtagagtg atgttttttt ctgccaactg cacggtgcgc
    19261 gcttcgctga cgcggcggaa taatgccgcc acatcaacag cagtcaaaac gacttcctga
    19321 cgattctcca gtcttgcctg gcgtagtaac gtttccacca gtgcctgcat tcgcgcattt
    19381 tgcgtcagaa tgttgtcagt aaaacgagcc actacttcag gcggcggacc ttcgcgtaaa
    19441 atttccgccg cgccacgaat cgccgccagt gggcttttta gctcatgggt taacgcataa
    19501 acatactgct caatatagtt tttcccttcc agcttcacgc gcatactttc cagcgcctgc
    19561 gcgagtttac gcagctcgct actaccgaga tcgggaagag gaacgggctt attgtcagtg
    19621 acggaatcag catagcgagt gagccgggca atggagcggt tgatccacca aaccatgcct
    19681 gcgccaatca ccagtgcgat ccccaacaaa atggcgctgg cccataaaat tcgccgctcg
    19741 ctgcgtttaa tgaccggagc catcgccgcg ttcggtttgc ctacgctcaa aacgccaata
    19801 agccgcgagc cgtccataat cggcgcggca acatacatca ccgaactttc gggatcggca
    19861 ggattttgca acgtgctacg cgcaccatac tgaccacgca acgttagcca gacgtcattc
    19921 cagcgcgaat aatcctgccc aacggcttta tttgccgaat cgaacaatac tttgccctgc
    19981 gcatcggtca tatagacatg atattcattg cgcactttgt taatgccacc gatattggcg
    20041 cgaaacgggc gatgttgtag ctgattaaac gcctgagcca gttgcccatg cgttgggtcc
    20101 ccagagagca aatcgggacg cgccagctcc gccagcaacg ttgcggtatc gattaacgtc
    20161 ccctccgttg ctcttcgcac gcccggttta acttctttga caaaaatagc cagtacgaac
    20221 caggccgcca ccgccaccag taaaaaatag cccagcagca accgcatgcc gatacgcatt
    20281 acaggcccct caggctatat cccatgccgc gatgagtatt aatcggagaa agatcggggt
    20341 tgatggcgcg cagcttggca cgcaacgttt taatgtgggt atcgacggtg cgatcgtagg
    20401 tgtcctgcgc atcttcccac acgctatcca ttagttgctg gcgggaccag acgcggcctg
    20461 gcgacttgag taacgtcttc agcaataaaa actcataccg cgtcagcgtt aatggcgtgt
    20521 caaaccagct gatctgcgcc gcgggttcat tcaattcaaa atgaccaata cggatgacgg
    20581 gagacggcga cgagaacttc ttcacccgac gcagtaaggt gcgcaccctg gcgcacactt
    20641 cgcggggtga aaacggttta gccacatagt cgtcagcgcc aatttccagc ccaagcaggc
    20701 gatcgacctc ttcacttcgg gccgtcagga acagtacagg taacgccgga tggagcgcca
    20761 gtaactggcg gcacaattca aagccgctga tatcaggcaa accaacatcg agaatcatga
    20821 cgtcgggaac ctgctggcga gctttatcca gcaccggcaa gcctcgctca aagacctcga
    20881 cggcaaaacc ttcctgctgc aacatgtaga ccagcgtgtc ggctatccct tgctcatctt
    20941 ccactaacca gaccgtttcc cgttgcataa atcgcctctg ttattgccgc cacggcatga
    21001 caggaaccgc gctaatcgcg tttttcggcg aaccttctac aactttgtcg gagtaagcca
    21061 gataagcgag cgcgttgcgt ttggcgtcat aaaagcgcac gacctgtaac gacttaaaga
    21121 ccagggacgt gcgttttttg aatactacct cgccctgagc tttgccgttt ttaatacgat
    21181 ccgacagttc aatcggcccg acttgctgac aagaaatagc cgcatcggag gtatcttccg
    21241 ccagacccaa tcccccttta ataccacctg ttttcgcccg gctcacataa caggtgacat
    21301 ttttcacatc gggatcgtca aaggcttcca caacaatttt gtgatccggg ccgatcattt
    21361 taaatacggt gtcgaccgaa ccaatctctt ctgcatgagc caatggcccc agcattatca
    21421 ggcttaaaga caggatcaaa tgcttgtatt tcatattgtt accattttta tctattacac
    21481 gagcaattag ttcgtcacgg taaataacat attgattaag aacaaaatct caatactttt
    21541 atttccgtca agccctaaaa catactctac taagaaaaaa acactgaatg ctaaaacagc
    21601 aaaaaatgct attatccaat tacctgatgt caggtgctcg ttgttgaaag gatgaggata
    21661 ttttatggat caggccggca ttattcgcga ccttttaatc tggctggaag gtcatctgga
    21721 tcagcccctg tcgctcgaca atgtagcggc gaaagcaggt tattccaagt ggcacttaca
    21781 gagaatgttt aaagatgtca ctggccatgc tattggcgcg tatattcgtg ctcgtcgttt
    21841 gtcgaaatcg gcggtcgcac tacgcctgac tgcgcgtccg attctggaca tcgcgctgca
    21901 ataccgcttc gactctcaac agacatttac ccgcgcattc aagaagcagt ttgcccagac
    21961 tcctgcactt taccgccgtt ctcctgaatg gagcgccttt ggtattcgcc cgccgctacg
    22021 tctgggtgaa ttcacaatgc cagagcacaa atttgtcacc ctggaagata cgccgctgat
    22081 tggtgttacc cagagctact cctgttcgct ggagcaaatc tctgatttcc gccatgaaat
    22141 gcgttatcag ttctggcacg attttctcgg caacgcgccg accattccgc cagtgctcta
    22201 cggcctgaat gaaacgcgtc cgagtcagga taaagacgac gaacaagagg tattctatac
    22261 caccgcgtta gcccaggatc aggcagatgg ctatgtactg acggggcatc cggtgatgct
    22321 gcagggcggc gaatatgtga tgtttaccta tgaaggtctg ggaaccggcg tgcaggagtt
    22381 tatcctgacg gtatacggaa cgtgcatgcc aatgctcaac ctgacgcgcc gtaaaggtca
    22441 ggatattgag cgatactacc cggcagaaga cgccaaagcg ggagatcgcc caattaatct
    22501 gcgctgtgaa ctgctgattc ctatccgccg ttaacgctgc agctcatcta atgcaggggc
    22561 gtccagatgc gagatgtccc ctgcggtttc aacgacccag ccagacgcca gccacaggct
    22621 ttcctgataa tccacgcgcg aaatagaaca gttacgcaga cgtaagcggc gctctgccca
    22681 tgctggtaat ccgagaatcg tactcaccag acatcccagt gcaataccat ggctcaccag
    22741 cagcggtcgg cttccctgcg gtaagtcccg gcaagattcc agtgccgcat tgacgcgatc
    22801 gctgagctcc tgcattgact cgccttcagg aatacgcccg tcaacggtgc cattgaccag
    22861 ctgccgacgc cagttctctt cttcttcggt cagagaatcg atatgtcttt tctccagcac
    22921 acccatgttt aattcacgca ggcgagaatc aaagatgatg tcacagccgc aggcctgggc
    22981 gatgatttcc gccgtacgcc gggtgcgtcc taaatcgcta ctgataatat gcgtaatgcc
    23041 aagctctttg gcacgggttg ccacctgcat cgcctgttgc tcacctttgg cggtcagcgg
    23101 gctgtcagac tggccctgaa tacgtcgctc ggcgttccac tgcgtttcac cgtggcggac
    23161 taggtatacc tgtaacatgc tttttttccg tatactgctg taacgttaat tttctgagta
    23221 atgctgatta tgcaccaagt tatctgtgcg accaccaatc ccgctaaaat tcaggccatt
    23281 ctgcaggcat ttcacgagat cttcggcgaa ggatcctgcc atattgcatc cgttgccgtc
    23341 gagagcggtg taccggaaca gccctttggc agtgaggaaa cgcgcgctgg cgcacgaaat
    23401 cgggtagcaa atgcccgccg tttacttcca gaggctgatt tttgggtggc gattgaagct
    23461 ggcatcgatg gtgacagcac tttcagttgg gtggtgattg aaaacaccag ccagcgcggc
    23521 gaggcgcgtt cggctacttt gccgttacct gcggtgattc tgcagaaagt gcgtgaaggt
    23581 gaggcgcttg gtcccgtgat gtcgcgttat accggtattg atgagattgg tcgtaaggaa
    23641 ggggcgatcg gcgtgtttac cgccgggaaa ctcactcgcg ccagcgtcta tcaccaggcg
    23701 gtgatcctgg cgctgagtcc gtttcacaat gccgtgtatc aggcattgta ggacggataa
    23761 ggcgttcacg ccgcatccgg cattcggtgc acgatgcctg atgcgacgct gccgcgtctt
    23821 atcaggccta caaaatcaat cgcttttcag caacacctct tccagccact ggcgcagctc
    23881 cacgggcgcg gctttcaggc tgttagatcc acgcgtaatc gtcgcgatgc ccgcgccgag
    23941 ttcatttttt aactcacgct gactcatttc gccacgcaac agctcttcga caatacgcac
    24001 gcgagtcccc aacgcttcgc gctcatctgg cgtcagcatc aggtttaaca acggtaaatg
    24061 gagatcgttt tggtaggcat tcttaagcag gtcgacaaaa cgtaaccact cctggtgacg
    24121 ctgttctgcc atcgctgctg aatagggtga ttgttgggcc ataatatgtc gccattgtta
    24181 gcgggggaag caaaatgcct cccccggttg tactcgctaa agagtacgat agcatatcat
    24241 aaacgtgcgg atcagtaacg acgtccccat tccgtggcgc tcatcaacgt cggtttatcc
    24301 cccatgaaat aacggtagta agcgtcataa gccagcacgt tcttcacata accgcgcgtc
    24361 tcggagaacg gaatactctc gacaaatgcc acagcgtcga tacgcccggc gctgttgcca
    24421 agccaggttc gcacccgtcc aggtccggcg ttataagctg ctgaggagaa aatacggtta
    24481 ttgccaaact gctgataaac atattgcagg taactggtgc caatgttgat attcgtttcc
    24541 ggatccagca attgcccagg gctgctataa ccaggaatag agaacatctt caccgtatgg
    24601 gtcgctgtac caggcataat ctgcatcagg ccgctggccc ctaccggtga tttcactttc
    24661 ggattccagg cgctctcctg acgggcaatc gccatcgcat agctttgcgg gatctctttc
    24721 ccactggtgt aacgtttgaa aagatcgttg taagccagcg ggaatcgctc ttccagatga
    24781 tcccaaagct tcccggcgat cgttgcctga acgctaagat cccaccattg gttattgaaa
    24841 gcataccgag ccagttgagc ctgctctgtt tttgacttgc tcttcaccag attggcccac
    24901 tcgctacgcg cggtgttatc gagattccag tacatcaact cgcgcacgcg cgccatctcc
    24961 ggcccctgag tcagggcgct gtcaacattc tgcggcgctt tatcaatctt cagctcatac
    25021 tcttctccga tgcgttgtgc tgcaaccatc gggtagaaac cacgctgttg catgagttga
    25081 tgcaaaatct ctttcgcttc agcttcacgt ccgcgttcca gcaataaatc cacctgccag
    25141 taacgccatt catctttctc ttttgcttcc atcggcagac gcgccagcca ggtattcagg
    25201 ccgcgacgat cgccggtgcc aagtgccatt cgcacacggc gttcaatcaa cgacgtggat
    25261 tgcgagcgca taatgacgtc atcgcgccat ttcgcctgct cgtcggtgac atcgttgccc
    25321 atcaaacgcc aggcgacgat atcgcgcagc tcctgaatct gatcttcatt aagctgctgc
    25381 acctgggcaa gcgacgggat catcagccgt gcattttcag catcctgccg cgccacactg
    25441 gcaaacgcca ccgctgccat ttgacgggta aaatcggtcg ctccggttgt acgcgcgaag
    25501 gtcagtaccg tattagggtt gttcgccagt gaaatgattg ccgaggcgat agtctggtaa
    25561 tcggcaggca tctgccctgc cagcactgtt accaggccag tgttacccgc tttcatcgcc
    25621 agacggatac gctctaaata cgccagcgga tcttgtttac ctgacgcacg ccagacgcta
    25681 aataacttgt cacaggcgtt aggctggctc ttgccggtta gccacagctc tttcgcccct
    25741 tgccaggctt cttcactctg cccggtgttc catttcgcat agtagtaatt acattgcgct
    25801 tcggtagttc cgggcttttc cgggctaaag gctaacaagc cacgccagtc ttcacgccgc
    25861 gccagttcat tgacgaaacg agattgcagc gtgcgagcgg gaggaagcgt ggggttagcg
    25921 cgaacaaagt tagtgaccgt caccgccggt tgattcatca gatcatcggt gatctggcgg
    25981 tattccaggt agggataaag cggataatcc ttcagtccag gcatcatttg ttcgaccaca
    26041 tccatttgtc gattatccca ggcctgcttg atttgcgcgt aacggctacg ctgctcatcc
    26101 agtgagtcgg ctcgcgccac gctgctgacc gtcagcagac agacaccggc agccaacagc
    26161 cgccaggtaa cttgtttggc tttttccaca agcgcatcct ctaagtgtaa atacatcaat
    26221 gcagcgtcat gccgcgtaat agatttatgc taaccagtca ttgccgttta cgccacgtta
    26281 cggacacttt tttactttta ctgcgaaggt gatcgaccaa ctgggattag gcggggaaaa
    26341 aggctacact tcgcctttga aaactcatca tcacgataaa catagaggcg aagtccaacg
    26401 tggctcaatt cgtttatacc atgcatcgtg tcggcaaagt tgttccgccg aaacgtcata
    26461 ttttgaaaaa catctctctg agtttcttcc ctggggcaaa aattggtgtc ctgggtctga
    26521 acggcgcggg taagtctacc ctgctgcgca ttatggcggg cattgataaa gacatcgaag
    26581 gtgaagcgcg tccgcagcca gacatcaaga ttggttacct gccgcaggaa ccgcagctga
    26641 acccggaaca caccgtgcgt gagtccattg aagaagcggt ttctgaagtg gttaacgccc
    26701 tgaaacgcct ggatgaagtg tatgcgctgt acgccgatcc ggatgccgat tttgacaagc
    26761 tggccgctga acaaggccgt ctggaagaga tcattcaggc tcacgacggt cataacctga
    26821 acgtacagct ggagcgtgcg gcggatgcgc tacgtctgcc ggactgggac gcgaaaatcg
    26881 ctaacctctc cggtggtgag cgtcgtcgcg tagcgttgtg ccgcctgctg ctggaaaaac
    26941 cagacatgct gctgctcgac gaaccgacca accacctgga tgccgaatcc gtggcctggc
    27001 tggaacgctt cctgcacgac ttcgagggca ccgtggtggc gattacccac gaccgttact
    27061 tcctcgataa cgttgcaggc tggatcctcg aacttgaccg cggtgaaggt attccgtggg
    27121 aaggcaacta ctcctcctgg ctggagcaga aagatcagcg cctggcgcag gaagcttcac
    27181 aagaagcggc gcgtcgtaag tcgatcgaga aagagctgga gtgggtacgt cagggaacta
    27241 aaggccgcca gtcgaaaggt aaagcacgtc tggcacgctt tgaagagctg aacagcaccg
    27301 aatatcagaa acgtaacgaa accaacgaac tgtttattcc acctggaccg cgtctgggcg
    27361 ataaagtgct ggaagtcagc aacctgcgta aatcctacgg tgatcgcctg ctgattgatg
    27421 acctgagctt ctcgatcccg aaaggggcaa tcgtcgggat catcggtccg aacggcgcgg
    27481 gtaaatcgac cctgttccgt atgatctctg gtcaggaaca gccggacagc ggcaccatca
    27541 ctttaggtga aacggtgaaa ctggcgtcgg ttgatcagtt ccgtgactca atggataaca
    27601 gcaaaaccgt ttgggaagaa gtttccggcg ggctggatat tatgaagatc ggcaacaccg
    27661 agatgccaag ccgcgcctac gttggccgct ttaactttaa aggggttgat cagggtaaac
    27721 gcgttggtga actttccggt ggtgagcgcg gtcgtctgca tctggcgaag ctgctgcagg
    27781 ttggcggcaa catgctgctg ctcgacgaac caaccaacga cctggatatc gaaaccctgc
    27841 gcgcgctgga aaacgccctg ctggagttcc cgggctgtgc gatggttatc tcgcacgacc
    27901 gttggttcct cgaccgtatc gccacgcaca tcctggacta ccaggatgaa ggtaaagttg
    27961 agttcttcga aggtaacttt actgagtacg aagagtacaa gaaacgcacg ctgggcgcag
    28021 acgcactgga gccgaagcgt atcaagtaca agcgtattgc gaagtaatgc gtaaaatgcc
    28081 ggatgcggcg tgaacgcctt atccggccta caaaccaaga aatttcaata cgttgtgaat
    28141 ctccaggcct gataagacgc ggtaagcgtc gcatcaggca ttgagcaccg aatgccggat
    28201 acggcgtgaa cgccttttcc ggcctacaac agccttagta tccgcaattt cgtagcgggt
    28261 cacccgtgca ggttttttca aaccttgttt aatctcgaca gcctcttcca gagaggtttt
    28321 caactcatca aaaaacttat tttatacctc gcctttgagc cgtttcatcg cggttatctc
    28381 tgctccccca tcatctcccg caccagctcc acgcagcgca ggaaacggct gtcataatcg
    28441 tcctcttcaa cccgcacgaa ttcgatattg ttctcttcca gcatctccac cagcaagttc
    28501 tggaactctt tgcgatccac cgaactgccg aggctgcgta aaccatccgc cacccacggc
    28561 gtgttgttct ccagcaggat caccagatcg aaacggtatt catcaatcaa cgcctgtacg
    28621 aacggatgct cacgcccttc gtactttttg cagaacgcct gggtagtgac aaaatcagtg
    28681 tcaataaacg ccactttatt ggcatatttc actgcaaaat caatgtattg tgcgtggccc
    28741 agcgcgattt tatcgtaatc ggaatactgc aatgcgatct catcaccgcc gaggtgtgaa
    28801 aagacataat cgcgaccata ttcccacgca ctggtggtat tgaagatatt ggcaagtttg
    28861 tttaccaggg tggatttacc gctcgactcg ccaccaagga tcgccacggt acgtacaaag
    28921 aacggcttca cttccgtagg aatatattcc cagtagcgga acgggttttc gcggatctgc
    28981 gcaccgctga tactcataaa ggtacgtttc ggatcgacca gcaccgtatc gatccccaga
    29041 tgttccatat actgtggcgc atcggcttct tccgaggtgt agatcaggtc cggctgaatc
    29101 cccttttcag ccataaactt tttgatgccg ttgctccaca catcccagcc gtgcggatac
    29161 ggctccatgc cctcttcgtt gaaagcatga atgcgaatat ttttctgata tttaaaagtt
    29221 tgtaataacc agcgcagacg atccggcacc gtgggctgct gcgacatggc gctgtcttca
    29281 aacaacgcgc ggtcgcgggt atcgtcaaaa cccataatga tatgcagctc gtcaacctgg
    29341 ctacaggcgc gctggataag gtagatatgt ccggtatgca gcgggtagaa cttaccgaat
    29401 acgacaccaa tggttttctt ctgtcgggga aattcaagcc ccaaaaaacg gtgcaacgcc
    29461 tccagctttt gcgcgctggg gcttttaatt ttggcattca gtaactggct taaataccct
    29521 ttggtcatac cgctggcatc agctacctgc tgtagcgtgc agccctgttg cttgatggca
    29581 gttttcaggt aatcaaatga cgacatatct ccctccgtat ctctcattat aagtcgtcga
    29641 acacgctaag cgcgtcggag agttttttaa cgccaaaaat ctgcatccct tccggcgctt
    29701 ttttcggtac gttagccgcc ggaacaatcg cccggcgaaa accgtgtttc gccgcttctg
    29761 aaattcgttc ctgaccgctg ggcaccgggc ggatctcccc tgccagcccg acttcaccaa
    29821 acaccaccag atcctgtggc agcggtctgt cacgcaggct ggaaaccatc gccagcagta
    29881 acgctaaatc ggcactggtt tcggttacct tcacgccgcc gaccacgttc acaaatacat
    29941 cctgatcggc catttgcaga ccaccgtgac ggtgcaagac agccagcagg attgccagac
    30001 ggttttgttc cagccccact gccacgcggc gcggattcgc catcatcgag tgatcgacca
    30061 gcgcctgaat ctccaccagc aacggacgcg ttccttccca caccaccatc acggagctgc
    30121 cggaggtcac ttcatctccg cgacttaaga aaattgccga agggttgctg acttcacgca
    30181 gcccctgttc ggtcatcgcg aagacgccca gctcattcac cgcgccgaag cggtttttat
    30241 ggctgcgcaa ggtgcgaaaa cgggagtcgg catcaccatc cagaagcacc gaacagtcga
    30301 tacagtgttc cagcacttta gggccagcca gcgagccatc tttggttacg tgcccaacca
    30361 tgacaatcgc cacaccgcgc gttttggcga agcgcgtcag ataagccgcc gtttcacgca
    30421 cctgcgccac gctaccaggc gatgattgta catccgccat atgcatcacc tggatcgagt
    30481 caattaccat cagtttcggt tgctcttctt cggcaatcag gcagatctgt tcgatgctgg
    30541 tttccgacaa catattgaga ttgtcagtcg gcaggccaag gcgatgagcg cgcattgcca
    30601 cctgttgcag cgactcttcg ccagtgacat acagcgtttt catctgctgg gccagtttgc
    30661 acagcgtttg cagtagcagc gtggatttcc ccgcacctgg gttaccgcca atcagaatgg
    30721 cgcttcccgg caccacgccg ccgcctagta cgcggtcgaa ctctttaaat ccggtggaaa
    30781 aacgcggcag ctcttcaagg ctgatatcgg agagtttctg gacttttgcc accccggcgc
    30841 taccggcata gccgctgaga cgctcgttac gcgccaccgt tggcgacgca gcaagacgca
    30901 cctcggtgat ggtgttccag gcatgacagg cactgcactg cccctgccag cgcggataat
    30961 cggccccgca ttcattacaa acaaaggcgc gttttggagc ttttgccacg gtgtacctcg
    31021 ttaatgctgt gccgcccgca ggatggcggg cgagcaatta cttctgattc aggctgccag
    31081 agaggatgca gaataccccc atcaggtcag cgtgacggat ggtgacttcc gccttttcat
    31141 tcacttttgg cttggcatgg taggcaatcc ccagccctgc tgctttgatc atcggcaggt
    31201 cattagctcc atcgccaatc gccacggtct gcgccagcgg gatttcatac tcctgcgcga
    31261 ggcgagtcaa agttttcgct ttgtactgcg cgtctacgat gtcgccaatc acattgccgg
    31321 taaatttacc gtccatgatc tccagttcat tggctaccac ggcggtcagg cgcagcttgt
    31381 cgcgcagata ttcagcaaag aaagtaaagc cgccggaggc aatcgccact ttccagccca
    31441 gcgtttccag cttgagcacc agttgcgtta agcctggcat cagcggcaga ttttcacgca
    31501 cctgttgcag aatattggcg tcagcgcctt tcagcgtcgc cacacggctg cgcaggctgg
    31561 cggtaaaatc gagttcgccg cgcatcgccc gttcggttac ttccgccacc atctcgcccg
    31621 ttccggccag tttggcaatt tcatcaatac attcaatctg gatggcggtg gaatccatgt
    31681 ccatcaccag caaacccggc gtgcgcaggt gcgggatttt ccccagcggg gcgacatcca
    31741 gttgcgcttc atgggccagg cgcgtagccc gtgcggtgag tgaacctgcc agacgaatca
    31801 cctgataatc ttccacgcac caggcggcaa caatcaccat cgccgcaccc agtttgctct
    31861 ggtattgggt cagacgctgt ttatccagcc cacgaccata cagcagccag ccgctacgac
    31921 ctgcgtggta atccagtggc atcacttcat caccacttaa tgaaagaggc agacccggcc
    31981 ataaagagac atcttcaggc aggtcgcacc aggtaatgtt aggcattaag gctcctgtaa
    32041 aatcgttcga agcagggaaa ataacgcatg aggctacctt gtatccattg cttctggcaa
    32101 cattaagtct caaattttca aagggtggaa gatggctcgc acaaaactga aattccggct
    32161 gcatcgggca gtgattgtcc tgttctgtct tgccttgtta gtggcactga tgcagggggc
    32221 gtcatggttt agtcaaaacc accagcgaca gcgtaatcca cagctggaag aactggcccg
    32281 caccctggcg cgtcaggtga cgctgaacgt tgcaccgctg atgcgtaccg actcaccaga
    32341 tgaaaaacgc attcaggcga tcctcgatca gttaacggat gaaagccgca tcctcgacgc
    32401 gggtgtgtat gacgaacaag gcgatcttat cgcccgttca ggcgaaagcg tcgaagtgcg
    32461 cgaccggcta gcgctcgacg gtaaaaaagc aggcggctat tttaaccagc agattgtcga
    32521 gccaattgcg ggtaaaaacg gaccgctcgg ctatctgcgc ctgacactcg acacccatac
    32581 gctcgccacc gaagcccaac aggtggataa caccactaac attttacgcc tgatgttgct
    32641 gctctcactg gcaatcggcg tagtgctgac ccgcacgctg ctacagggta aacgcacccg
    32701 ctggcagcaa tcgcccttcc tgttaaccgc cagcaaaccg gtgccggaag aggaagaaag
    32761 cgagaaaaaa gagtgaccca ttactacaag aaaggaaatc gttatgtcca cattacgcct
    32821 gctcatctct gactcttacg acccgtggtt taacctggcg gtggaagagt gtatttttcg
    32881 ccaaatgccc gccacgcagc gcgttctgtt tctctggcgc aatgccgaca cggtagtgat
    32941 tggtcgcgcg cagaacccgt ggaaagagtg taatacccgg cggatggaag aagataacgt
    33001 ccgcctggcg cggcgcagta gcggtggcgg cgcggtgttc cacgatctcg gcaatacctg
    33061 ctttaccttt atggctggca agccggagta cgataaaacc atctctacgt cgattgtgct
    33121 caatgcgctg aacgcgctcg gcgtcagcgc cgaagcgtcc ggacgtaacg atctggtggt
    33181 gaaaacagtc gaaggcgacc gcaaagtctc aggctcggcc tatcgcgaaa ccaaagatcg
    33241 tggcttccac cacggcactt tgctgctcaa tgccgacctt agccgcctgg caaactatct
    33301 caatccggat aaaaagaaac tggcggcgaa aggcattacc tcagtgcgtt cccgcgtgac
    33361 caacctcacc gagctgctgc cggggatcac ccatgagcag gtttgcgagg ccataaccaa
    33421 ggcctttttc gcccattatg gcgagcgtgt agaagcggaa atcatctccc cggacaaaac
    33481 gccagacttg ccaaacttcg ccgaaacctt tgcccgtcag agtagctggg aatggaactt
    33541 cggtcaggct ccggcattct cgcatctgct ggatgaacgc tttagctggg gcggcgtgga
    33601 actgcatttc gacgttgaaa aaggccatat cacccgcgcc caggtgttta ccgacagcct
    33661 caaccccgcg ccgctggaag ccctcgccgg gcgactgcaa ggctgcctgt accgcgcgga
    33721 tatgctgcaa caagagtgcg aagcgctgtt ggttgacttc ccggaccagg aaaaagagct
    33781 acgggagttg tcgacgtgga tagcgggggc ggtaaggtaa ctcgctgatt aaaataccaa
    33841 aggaagggaa tatgatcgat ctggaaaacc aggagcggga gattatcaat ttaatgttaa
    33901 gccagcgtat ttcctggcta gccgccgtgc gtatcaggca taaattatca cttgctgaag
    33961 taagtaaaat gttaggtatc tcgattaatt ctctaaaata gatcgaaaag acggaacggc
    34021 ttagctcaaa catcaaaagc aaaatgggag aaatttatgg ttgtccacca gagttgctga
    34081 tatgcccttc ctggatgacg gctgaacata aatgaataga ttcgtgaata gatacttgaa
    34141 tgtattttat tttttatcca tatgaaaaat aatagtaaat ctataaatca gttgctattg
    34201 atgaatagat actaaaaagc cggagcagtc ccccactccg gcttaacacg acaatcgcct
    34261 ttcagcgaaa cacaattact ctttatcgcc cagcagaacg gattccagtg cgattttgat
    34321 catgtcgttg aaggtggtct gacgctcagc ggcagtggtc tgctcgtgag tgcggatgtg
    34381 gtcagacaca gtgcagatgg tcagggcttt cgcgccaaat tctgcagcga cgccgtagat
    34441 accagccgct tccatttcca cgccgaggat gccgtatttt tccatcacgt cgaacatttc
    34501 gccgtccgga gagtagaaca ggtcagcgga gaacaggtta ccaacgcgag catcaatgcc
    34561 cagtgcttta gccgcgtcta ccgcgttacg caccatgtca aagtcagcga tagcggcaaa
    34621 gtcatgatct ttaaaacgga tgcggttaac tttggaatcg gtgcaggcac ccataccgat
    34681 aacgacgtcg cgcagtttta cgtgcggcag aactgcgcca caggaaccca cgcggataat
    34741 tttcttcacg ccgaaatcgg tgatcagttc tttggtgtag atggagcagg acgggatacc
    34801 cataccgtga cccattacgg aaattttgcg gcctttgtaa gtaccggtga agcccagcat
    34861 accgcgaacg ttgttcactt cacgggcatc ttcaaggaaa gtttcagcaa tatacttcgc
    34921 acgcagcggg tcgcctggca tcaaaactac gtcagcgaaa tcgcccattt ctgcattaat
    34981 gtgtggggta gccattgttt tatccttatc aacatgtttt caccctctcc ctgagggaga
    35041 gggccggggt gagggcatca agccgcaccc attatcagaa catggctttg ccatattcca
    35101 tatcagaagt accaaaatat tttgccagag tctggccgat atccgcgaag gtttcacggt
    35161 gacccagtga gcccggtttt actttcgggc cgtaaaccag taccggaatg tgttcacgcg
    35221 tgtggtcagt accggtccag gtcggatcac aaccgtggtc agcagtgagg atcaggatgt
    35281 cgtcatcgcg cagcagagac atcagctccg gcagacggcg gtcgaacagc tccagaccca
    35341 cagcataacc ggcgacgtcg cgacggtggc cccaggaaga gtcgaagtca acgaagttgg
    35401 tgaagacgat ggtgttatca cccgcttctt tcatctcttt gatggtggcg tcaaacagcg
    35461 cgtccaggcc agttgctttc actttcttgg tgataccgca gttggcgtag atgtctgcaa
    35521 ttttaccgac agaaaccacc tgaccgtgtt tttcatcaac cagtttctgc agcacggtcg
    35581 gtgctggcgg ctcaacagcc aggtcgtgac ggttaccggt acgctggaag ttaccggctt
    35641 tgtcaccgat aaacggacga gcgataacac gaccgatgtt gtagccgccg ttggtcagct
    35701 cttcacgggc gatttcgcac agttcgtaga gtttatccag gccaaaggtt tcttcgtggc
    35761 aggcaatctg gaacacagag tcagcggagg tatagaaaat cggcttgccg gttttcatgt
    35821 gctcttcgcc cagctgatcc agaatgaccg taccggaaga gtggcagtta ccgaggtaac
    35881 ccggcagatt agcgcgttca accagtttat ccagcagctc ttgcgggaag ctgttttcgt
    35941 gatcggagaa atatccccac tcaaatagaa ccgggacacc ggctatttcc cagtgaccag
    36001 acggggtatc tttaccggat gacatttcgt gcgcccatgc gtacgcgccg ataacttcag
    36061 cgttgccgtc cattcccgcc ggaatgaaac cggtagaacc ttcgtgtgct ttcgccagcc
    36121 ccagacgggt cagatttggc agattgagcg gacctttacg accgttatca gcttcgcctt
    36181 tggcacaagc ttctgcgata tgacccaggg tgtcagcccc gacgtcacca aagcgttctg
    36241 catcttctgt agcgccgatg ccgaatgagt ccagcaccat aataaatgca cgtttcatat
    36301 gttctccgta ctcagtgctt caaatttaaa tgatcagatc agtataccgt tattcgctga
    36361 tacggcgata gacagttggt gtgctttccg gtgctttatc ggcaagttta attgccgctt
    36421 tcaccgcttt cgccgcttcc tgccagctgt tttcgtcttt cgcgtggata accgccagcg
    36481 gacgctgacc gtctacctgg tcgcccaaac gcgccatatc agtaaagccg acgctgtaat
    36541 caatggtgtc agatgcctga cgacgaccgc cgcccattgc aaccactgcc atccccagcg
    36601 cgcgggtatc catttcactg acaaaacctt cggtatcagc atagactgct ttcgtcagca
    36661 tcgctgtcgg cagatacttc gcgtagttct caacgaagtc ggttgggcct ttttgtgccg
    36721 ctaccatacg accaaagact tctgccgctt taccgttgtc cagcactgct tgcaatttcg
    36781 cgcgcgcttc ggcgtcatct ttcgccagtt tgccggagat aagcatctcc acgcacagcg
    36841 ccatcgtgac atcaaacaga cgcgggttac gatactcacc cgtcaggaac tgcaccgctt
    36901 cacgaacttc aaccgcgtta cctgcactgg aggccagtac ctgattcatg tcggtgagca
    36961 gcgcggtggt gcgcacgcca gcgccgttag ccacgccaac aatcgcttcg gcaagggctt
    37021 cagagagttc gtaggtcggc ataaacgcgc cgctacccac tttcacgtcc atcaccagcg
    37081 cgtccagacc ttccgcaagt ttcttcgcca gaatagaggc ggtgatcagc gggatggagt
    37141 ccacggttgc ggtaatatca cgggtcgcgt agaaacgttt atccgccgga gccagtgagc
    37201 tggtctgacc gataatcgcc acgccgacgt ctttaataat ttcgcggaaa cggttgtcat
    37261 ccgggaaaat gtcgaagcca gggatggatt ccagtttgtc gagcgtaccg ccagtatgac
    37321 cgaggccgcg accagagatc atcggaatat agccgccgca ggctgcgacc atcggcccca
    37381 acatcagcga agtcacatcg ccgacgccac cagtggaatg tttatcaaca atcgggccat
    37441 tcagatgcag gcttttccag tcgagaacgg ttcctgaatc tcgcatcgcc atggtcagcg
    37501 agacacgctc aggcattgtc atatcgtgga agaaaatggt catcgcgagg gcggcaatct
    37561 gcccttcgga gatagtgttg tcgcgaatgc cgttgataaa gaaacgaatt tcttcatcgc
    37621 tcagtgcatg accatcacgt tttttacgaa taatttcttg tgcgagaaac aaggtaacct
    37681 cccgaggaaa agagtatgag gcggcttttg ccggatgcgt ctacgcctta tccggcctac
    37741 gggtagatac gtctcgtagg cctgataagc gcagcgcatc aggcgtaaag catcttactt
    37801 agtagctgct ggcgctctta ccgtcaccgt ggcccagcgc tttcaacagg cttgccagca
    37861 ggctggaagc accaaagcgg tagtgacgcg catctgccca gtcagcaccg aacagttcat
    37921 ctgcgatggc gagatatttc tgcgcatctt ccgcagtacg cacgccgccc gctggtttga
    37981 aaccaacggt tttttctacg cccatatcac ggatcacttc catcatgatg cgcgcgcttt
    38041 ccggcgtcgc gttcacagcc actttaccgg tagaggtttt gatgaagtcc gcacccgctt
    38101 tgatggagat ttcagacgct ttacggatca gcgcttcgtc tttcagttcg ccggtttcga
    38161 tgatcacttt cagcagtaca ttcgctgccg cgcaagcctc tttacaggct ttcaccaggt
    38221 caaaaccaac ctgctcgtta cccgccatca gcgcgcggta cgggaacaca acgtcaactt
    38281 catcagcacc gtaggcgatt gccgcacggg tttctgccag cgcgatttcg atgtcatcgt
    38341 taccgtgtgg gaagttggtt accgtagcga ttcggatttc cggggtgccc tgctctttca
    38401 gcgttttgcg agcaatcggg ataaagcgag gatagataca gatagcggcg gtattgccga
    38461 ccggggtttt ggcctgatga cacagagcaa ttactttctc gtcggtgtcg tcgtcattca
    38521 gggtggtcag gtccatcaat ttcagtgcac gcaggctgct tgctttcaga tcagtcattt
    38581 cattctcctg gcttgtcgcc aataaaattc accttgcgag tttgttagta ttctaacatc
    38641 tactccgcaa cacacttcga tatacatcac aattaaggaa atctacttac aagtttgcat
    38701 cactgtaatg cgatctggtt caaataattc actttcaaat gaatgcgtca gtggtggcaa
    38761 acgcatcagg atcaaagtga acatcacgaa acttcttaca atggcgcatc accttcggca
    38821 taaaaggaaa agaagatgcc cacttctcat gaaaatgcac tgcaacaacg ttgccagcaa
    38881 attgtcacca gcccagtgct tagcccggag cagaagcgcc attttctggc actggaagca
    38941 gaaaacaatc tgccttatcc gcagcttcct gccgaagccc gccgcgcgct ggatgagggt
    39001 gtaatctgcg atatgtttga aggtcatgcg ccgtacaaac cgcgctatgt cttacccgat
    39061 tacgcccgtt ttctggcgaa cggttccgaa tggctggagc tggaaggcgc gaaagatctt
    39121 gatgacgcac tctctctgct gaccattctt taccaccacg taccgtcggt cacatcgatg
    39181 ccggtctacc tggggcaact ggatgcgttg ttgcaaccat atgttagaat tctaacacaa
    39241 gacgagatcg atgttcgaat aaaacgtttc tggcgttacc tcgacagaac cctgccagac
    39301 gcctttatgc acgccaatat cggcccgtct gattcgccca ttacccgcgc gatcttacgt
    39361 gcagacgcag agttgaagca ggtttcaccg aacctgacct ttatctacga tcctgaaatc
    39421 acccctgatg acctgctgct ggaagtggcg aagaacatct gtgaatgtag caaaccgcac
    39481 atcgccaacg gtccggtgca tgataaaatt ttcacaaaag ggggctacgg gattgtgagc
    39541 tgttacaact cactgccgct ggcgggtggt ggcagcacgc tggtacgcct taacctgaaa
    39601 gccattgccg agcgcagcga atcgctggat gacttcttta cgcgcactct accgcactac
    39661 tgccagcagc agatcgccat catcgatgcg cggtgtgaat tcctctatca acaatcacac
    39721 ttctttgaga atagcttcct ggtgaaagaa gggctgatta accctgaacg ttttgtgcca
    39781 atgtttggca tgtatgggct ggcggaagcg gttaacttgc tgtgtaagaa agaagggatt
    39841 gccgcgcgct acggtaaaga agccgccgca aatgaagtgg gttatcgcat cagcgcgcaa
    39901 ctggcggagt ttgtcgccaa tacccccgtg aaatatggct ggcaaaaacg cgccatgtta
    39961 cacgcacagt cggggagcag ttccgatatc ggcaccacgc cgggcgcgcg tttgccgtat
    40021 ggtgatgagc cagatccgat cacccatctg caaactgtcg ccccgcatca tgcttattat
    40081 tattccggca tcagcgacat tctgacgctc gacgaaacca tcaaacgtaa tccgcaggcg
    40141 ctggtacagc tttgcctcgg tgcctttaaa gccggaatgc gtgaatttac tgccaatgtc
    40201 agcggtaacg atctggttcg cgttaccggt tatatggtgc gtttgtcgga tttagaaaaa
    40261 tatcgcgccg aaggttcacg caccaacacc acctggctgg gcgaagaagc cgcacgcaac
    40321 actcgtattc tggaacgcca gccgcgcgtg ataagccatg aacagcagat gcgctttagt
    40381 cagtaagatt atccccttct cctgcgttga cgggccaggc agtcgtctgg ctctgttttt
    40441 gcagggctgc aatctgcgct gcaaaaactg tcacaatccg tggacgatgg gacgttgcaa
    40501 tgactgtggg gagtgcgtgc cacagtgtcc gcatcaggcg ttgcagattg ttgacggcaa
    40561 agtggtgtgg aacgctgtgg tttgcgagca gtgtgatacc tgcctgaaga ggtgtccgca
    40621 acatgccacg ccaatggcgc aatccatgag cgtggacgaa gtgcttagcc atgtccgcaa
    40681 agcggtgctg tttattgaag ggataacggt aagcggcggc gaagccacga cccaactgcc
    40741 gtttgtagtg gcgctgttta ctgctatcaa aaacgatccg caactgcgcc acctcacctg
    40801 cctggtggac agtaacggca tgttgagcga aaccggctgg gaaaaattac tcccggtgtg
    40861 tgacggcgca atgctcgatc tcaaagcgtg ggggagcgaa tgtcatcaac acttaaccgg
    40921 acgcgataat cagcagatta agcgcagcat ctgtttgctt gcagagcgcg gcaagctggc
    40981 ggaactgcgt ttgctggtaa ttccagacca ggtggattat ttgcatcaca tcgatgagct
    41041 ggcgacgttt atcaagagac ttggcgatgt tccggttcgc ctgaatgcgt ttcatgccca
    41101 cggcgtgtat ggcgaggcgc aaagctgggc gagcgccacg ccggaagacg ttgagccgtt
    41161 ggctgatgcg ttaaaggtgc gcggggtgag ccggttgata tttccggcgc tctatttgtg
    41221 acatgccgga tacggcctac ggttcggcac agacttgtag gcatgataag acgcgacaag
    41281 cgtcgcatca ggcattatgc gcc
//
LOCUS       DAJVQG010000015        40833 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJVQG010000015 DAJVQG010000000
VERSION     DAJVQG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40833
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.015"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..687
                     /locus_tag="OQM83_000772"
     CDS             <1..687
                     /locus_tag="OQM83_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HCS1519107.1"
                     /translation="RREYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYY
                     DPLQGRYITQDPIGLKGGWNLYTYPLSPVNSMDPLGLYEFKSKNIDDIGIFALAMCNG
                     ESINENKEYGGLICKKQGEYFPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYS
                     DDKGNKVTKENDVYDSLNFSSKDLTNSYMNGMGKKEYSSYLGTPNNTYLKYNPKAKGN
                     GVTIIRQGSN"
     gene            689..925
                     /locus_tag="OQM83_000773"
     CDS             689..925
                     /locus_tag="OQM83_000773"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1519108.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSNSDSAALYCVAKIF
                     PDGENILIYKVNIINASSKKDVFFMKAQHQPTMT"
     gene            complement(1098..1773)
                     /locus_tag="OQM83_000774"
                     /pseudo
     CDS             complement(1098..1773)
                     /locus_tag="OQM83_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1918..2526)
                     /locus_tag="OQM83_000775"
     CDS             complement(1918..2526)
                     /locus_tag="OQM83_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCS1519109.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYPPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(2710..3027)
                     /gene="metJ"
                     /locus_tag="OQM83_000776"
     CDS             complement(2710..3027)
                     /gene="metJ"
                     /locus_tag="OQM83_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCS1519110.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            3304..4464
                     /gene="metB"
                     /locus_tag="OQM83_000777"
     CDS             3304..4464
                     /gene="metB"
                     /locus_tag="OQM83_000777"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCS1519111.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            4467..6899
                     /gene="metL"
                     /locus_tag="OQM83_000778"
     CDS             4467..6899
                     /gene="metL"
                     /locus_tag="OQM83_000778"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCS1519112.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            7248..8138
                     /gene="metF"
                     /locus_tag="OQM83_000779"
     CDS             7248..8138
                     /gene="metF"
                     /locus_tag="OQM83_000779"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCS1519113.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            8467..10647
                     /gene="katG"
                     /locus_tag="OQM83_000780"
     CDS             8467..10647
                     /gene="katG"
                     /locus_tag="OQM83_000780"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCS1519114.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            10741..11646
                     /gene="yijE"
                     /locus_tag="OQM83_000781"
     CDS             10741..11646
                     /gene="yijE"
                     /locus_tag="OQM83_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCS1519115.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(11673..12290)
                     /locus_tag="OQM83_000782"
     CDS             complement(11673..12290)
                     /locus_tag="OQM83_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCS1519116.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(12564..13667)
                     /gene="gldA"
                     /locus_tag="OQM83_000783"
     CDS             complement(12564..13667)
                     /gene="gldA"
                     /locus_tag="OQM83_000783"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HCS1519117.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(13678..14340)
                     /gene="fsa"
                     /locus_tag="OQM83_000784"
     CDS             complement(13678..14340)
                     /gene="fsa"
                     /locus_tag="OQM83_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCS1519118.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(14352..16853)
                     /gene="ptsP"
                     /locus_tag="OQM83_000785"
     CDS             complement(14352..16853)
                     /gene="ptsP"
                     /locus_tag="OQM83_000785"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1519119.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            17162..18241
                     /locus_tag="OQM83_000786"
     CDS             17162..18241
                     /locus_tag="OQM83_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCS1519120.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTGGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            18256..18576
                     /locus_tag="OQM83_000787"
                     /pseudo
     CDS             18256..18576
                     /locus_tag="OQM83_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            18627..20923
                     /locus_tag="OQM83_000788"
                     /pseudo
     CDS             18627..20923
                     /locus_tag="OQM83_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            20889..21767
                     /locus_tag="OQM83_000789"
     CDS             20889..21767
                     /locus_tag="OQM83_000789"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCS1519121.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            21769..22110
                     /locus_tag="OQM83_000790"
     CDS             21769..22110
                     /locus_tag="OQM83_000790"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCS1519122.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(22097..22948)
                     /locus_tag="OQM83_000791"
     CDS             complement(22097..22948)
                     /locus_tag="OQM83_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1519123.1"
                     /translation="MYHGVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(23163..24896)
                     /locus_tag="OQM83_000792"
     CDS             complement(23163..24896)
                     /locus_tag="OQM83_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCS1519124.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(25079..27730)
                     /gene="ppc"
                     /locus_tag="OQM83_000793"
     CDS             complement(25079..27730)
                     /gene="ppc"
                     /locus_tag="OQM83_000793"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCS1519125.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(28082..29233)
                     /gene="argE"
                     /locus_tag="OQM83_000794"
     CDS             complement(28082..29233)
                     /gene="argE"
                     /locus_tag="OQM83_000794"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCS1519126.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPSMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            29387..30391
                     /gene="argC"
                     /locus_tag="OQM83_000795"
     CDS             29387..30391
                     /gene="argC"
                     /locus_tag="OQM83_000795"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCS1519127.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            30399..31175
                     /gene="argB"
                     /locus_tag="OQM83_000796"
     CDS             30399..31175
                     /gene="argB"
                     /locus_tag="OQM83_000796"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCS1519128.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            31236..32609
                     /gene="argH"
                     /locus_tag="OQM83_000797"
     CDS             31236..32609
                     /gene="argH"
                     /locus_tag="OQM83_000797"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCS1519129.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALSVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            32876..33793
                     /gene="oxyR"
                     /locus_tag="OQM83_000798"
     CDS             32876..33793
                     /gene="oxyR"
                     /locus_tag="OQM83_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCS1519130.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPIADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(33776..35176)
                     /gene="sthA"
                     /locus_tag="OQM83_000799"
     CDS             complement(33776..35176)
                     /gene="sthA"
                     /locus_tag="OQM83_000799"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCS1519131.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            35497..36147
                     /gene="fabR"
                     /locus_tag="OQM83_000800"
     CDS             35497..36147
                     /gene="fabR"
                     /locus_tag="OQM83_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCS1519132.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            36147..36506
                     /locus_tag="OQM83_000801"
     CDS             36147..36506
                     /locus_tag="OQM83_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCS1519133.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(36546..37646)
                     /gene="trmA"
                     /locus_tag="OQM83_000802"
     CDS             complement(36546..37646)
                     /gene="trmA"
                     /locus_tag="OQM83_000802"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCS1519134.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      37760..37950
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            38015..39859
                     /gene="btuB"
                     /locus_tag="OQM83_000803"
     CDS             38015..39859
                     /gene="btuB"
                     /locus_tag="OQM83_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCS1519135.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRGTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            39804..40661
                     /gene="murI"
                     /locus_tag="OQM83_000804"
     CDS             39804..40661
                     /gene="murI"
                     /locus_tag="OQM83_000804"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCS1519136.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9435 a        10167 c        11216 g        10015 t
ORIGIN      
        1 cgccgggaat atgatgaatg gggcaacctg ctgaatgaag agaacccgca tcagctgcag
       61 cagcttattc gtttgccggg gcagcagtat gatgaggagt ccggcctgta ttataaccgc
      121 caccgctatt atgacccgct gcaggggcgg tatatcactc aggatccgat tgggctgaag
      181 gggggatgga acctgtatac atatccgctg agcccggtga atagcatgga tccattagga
      241 ttatatgaat ttaaatcaaa aaatattgat gatattggaa tatttgcatt ggcaatgtgt
      301 aatggagaat caattaacga gaataaagaa tatggtggac taatatgtaa gaagcaaggt
      361 gaatatttcc ccatgaatcc gataagttca aatgataatg atagtgtaga cttgcgaaat
      421 ataaaatgcc ctgaaggttc agagagagta ggcgattatc acactcacgg tttttactct
      481 gacgataaag gaaataaagt aacaaaagaa aatgatgttt atgatagtct aaatttttca
      541 agcaaagatt taacgaattc ttatatgaat ggaatgggaa aaaaagaata cagtagttac
      601 ttgggaacac caaataacac ctatctaaaa tataatccca aagctaaagg gaatggagtt
      661 acaattatca ggcaagggag taattaaaat gaaggcaata caatgttgtt atttccttgc
      721 tttagtctcc agtcatttaa tggctggagt tatgggtgat gataatatat ttttaagtaa
      781 ttcggattca gctgctttat attgtgtggc taaaatattt ccagatggag aaaatatttt
      841 aatatataaa gtaaatataa ttaatgctag ttcaaaaaaa gatgtttttt ttatgaaagc
      901 ccaacatcaa cccacaatga cataatgggg ggcgggggaa tgtcatatgc ctatgaatat
      961 gatgtaaaat ataatatatg tcatttgaga aaaaagagca gaatgaggta aaaatctttg
     1021 gctgtgtatt cagtctggta atcatccaag gaaagcttat cagaagggtg atgactccaa
     1081 ttagtagaac atatattttc gataaaagca ccgatgactt tttcatgaag ttctacagaa
     1141 cgcgagaaac agatggttct gcgggccaga cgtttgattc gggttctcag ggtcagattg
     1201 ttacgctcaa tgtgctgagt aaaaatctta ccagtcagat gcttctcctt cggtaactct
     1261 ctagcgtaac taccccattc gtcagtggtc aacatgccga tggtaaaagg gtaagcagcg
     1321 cccgcaattc acgacaggtt tcgtcagtcc gggggccaaa agtgtaggcc agaacaccac
     1381 cagttttcgt gttgtacacg taccagagcc agtgctgtcg cgctttactg ccgacaaagc
     1441 tccattgctg actaagtttg cagatgagtg ccacatcagc atgagcgacc ggggatgacg
     1501 ttattcttcg tggcgagagt tttttaaagt tcggatgact gtattaatgc cgattttcag
     1561 cgtacgggca gtatcgcgaa ccccggcacc gttgaaagtc atttcagcga tttggtcttt
     1621 aactcccggt ttacggggct cgtaacagta actgagctga aatacccggt gacagtcacg
     1681 gccgcggaat cgctcatgtc tttttgggtt ctgcccgtga cggtaaacct gaacggactg
     1741 gcaacggggg caatgaaggt taactgtgac catgaaacaa ccttaaaagc gagtattata
     1801 tccttaattc aactaattgg aggcatcacc gtttaccgga gcgacagctt gcggaaaggt
     1861 cgtgcaacta caaagcaaca aacagactac ggcgggaatt atttttttca tcatccatta
     1921 ccacgaaaac agcggccact ctttggcgac tgtggtgagt tgtggcgcgt cgaaaacggc
     1981 ttgtggtgaa actaccgttt cctcaagcgg aatatttttg ccgtagcgtt ctttgagctt
     2041 agcctggacc agcgggttgc tgagatcgaa ctctttcaac ttctgctgct cgcccacgcg
     2101 cattcccagc gcgttctggt aaaccttcca caccacttcc gaacagtact ggcgatcgtc
     2161 gctccatgag aagctgaaat catacggttt accgagataa cgttttgccg tttgcgccag
     2221 tttttgctgt tgttcaacac tcagtccgcc ttcaacgcgg cgaacaacgt atttgccctt
     2281 ttcaccatgg gcgatccact gcttgagcgg ggggtatttc accgggccga ccgcttcaaa
     2341 aacgtagggc tttttgttgc gtatcaccag cataccggtg tggctataat cggaatgggt
     2401 cgccagttgg atcgctttac tttgcgatga gcgagagatc tgaaagatga tgtcgccggt
     2461 ttgtggctgc caggcaaagg caggtacaga aaccagcagg ctgaggatca gcagcctgtt
     2521 tttcatagtt aaacgtccat gtataaaaag cggtgggtcg cagacaacgt gctcgttgtt
     2581 tatgccggat gcggcgtgaa cgccttatcc ggcctacaag ttcgtgcaaa ttcaataaat
     2641 tgcaatatga cgtaggcctg ataagcgtag cgcatcaggc gattccactc cgcgccgctc
     2701 ttttttgctt tagtattccc acgtctccgg gttaatcccc atctcacgca tgatctcttt
     2761 tgccgcttcc gggatttcgt cgctgcgctc tttacgcaga tcggcatcat ccggcaaagg
     2821 ttgcccggta aaggcatgca gaaacgcttc gcacagcagc tcgctgttgg tagcgtgacg
     2881 caggttgttc acctgacgac gcgtgcgttc atcggtgagg atttttaaca ccttaagagg
     2941 aatggaaacc gtaatctttt tgacttgttc actcttcttg ccgtgctcag cgtatgggct
     3001 gatatattcg ccgctccatt cagccatgag atacttaatc ctcttcgtca ataaattgag
     3061 accagaccac acaatttatg tgggtactga ccgtaaaccc gcatagttta ccgtacaggc
     3121 gttaccgtga catcgtgtaa tgcacctgtc ggcgtgataa tgcatataat tttaacggct
     3181 atttgggatt tgctcaatct atacgcaaag aagtttagat gtccagatgt attgacgtcc
     3241 attaacacaa tgtttactct ggtgcctgac atttcaccga caaagcccag gaaacttcat
     3301 cacatgacgc gtaaacaggc caccatcgca gtgcgtagcg ggttaaatga cgacgaacag
     3361 tatggttgcg ttgtccctcc catccatctt tccagcacct ataactttac cggatttaat
     3421 gaaccgcgcg cgcatgatta ctcgcgtcgc ggcaacccaa cgcgcgatgt ggttcagcgt
     3481 gcgctggcag aactggaagg cggtgctggc gcagtactta ctaataccgg catgtccgcg
     3541 attcacctgg taacgaccgt ctttttgaaa cctggcgatc tgctggttgc gccgcacgac
     3601 tgctacggcg gtagctatcg cctgttcgac agtctggcga aacgcggttg ctatcgcgtg
     3661 ttgtttgttg atcaaggcga tgaacaggca ttacgggcag cgctggcaga aaaacccaaa
     3721 ctggtactgg tagaaagccc aagtaatcca ttgttacgcg tcgtggatat tgcgaaaatc
     3781 tgccatctgg caagggaagt cggggcggtg agcgtggtgg ataacacctt cttaagcccg
     3841 gcattacaaa atccgctggc attaggtgcc gatctggtgt tgcattcatg cacgaaatat
     3901 ctgaacggtc actctgacgt ggtggctggc gtggtgattg ctaaagaccc ggacgttgtc
     3961 actgaacttg cctggtgggc aaacaatatt ggcgtgacgg gcggcgcgtt tgacagctat
     4021 ctgctgctac gcgggttgcg aacgctggtg ccgcgtatgg agctggcgca gcgcaacgcg
     4081 caggcgattg tgaaatatct gcaaacccag ccgttggtga aaaaactgta tcacccgtcg
     4141 ctgccggaaa atcaggggca tgaaattgcc gcgcgtcagc aaaaaggctt tggcgcaatg
     4201 ttgagttttg aactggatgg cgatgagcag acgctgcgtc gtttcctgag cgggctgtcg
     4261 ttgtttacgc tggcggaatc attaggggga gtggaaagtt taatctctca cgccgcaacc
     4321 atgacacatg caggcatggc accagaagcg cgtgctgccg ccgggatctc cgagacgctg
     4381 ctgcgtatct ccaccggtat tgaagatggc gaagatttaa ttgccgacct ggaaaatggc
     4441 ttccgggctg caaacaaggg gtaaaaatga gtgtgattgc gcaggcaggg gcgaaaggtc
     4501 gtcagctgca taaatttggt ggcagtagtc tggctgatgt gaagtgttat ttgcgtgtcg
     4561 cgggcattat ggcggagtac tctcagcctg acgatatgat ggtggtttcc gccgccggta
     4621 gcaccactaa ccagttgatt aactggttga aactaagcca gaccgatcgt ctctctgcgc
     4681 atcaggttca acaaacgctg cgtcgctatc agtgcgatct gattagcggt ctgctacccg
     4741 ctgaagaagc cgatagcctc attagcgctt ttgtcagcga tctggaacgc ctggcggcgc
     4801 tgctcgacag cggtattaac gacgcagtgt atgcggaagt ggtgggccac ggggaagtat
     4861 ggtcggcacg cctgatgtct gcggtactta atcaacaagg gctgccagcg gcctggcttg
     4921 atgcccgcga gtttttacgc gctgaacgcg ccgcacaacc gcaggttgat gaagggcttt
     4981 cttacccgtt gctgcaacag ctactggtgc aacatccggg caaacgtctg gtggtgaccg
     5041 ggtttatcag ccgtaacaac gccggtgaaa cggtgctgct ggggcgtaac ggttccgact
     5101 attccgcgac acaaatcggt gcgctggcgg gtgtttctcg tgtaaccatc tggagcgacg
     5161 tcgccggggt atacagtgcc gacccgcgta aagtgaaaga tgcctgtctg ctgccgttgc
     5221 tgcgtctgga tgaagccagc gaactggctc gtctggcggc tcccgttctt cacgcccgta
     5281 ctttacagcc ggtttctggc agcgaaatcg acctgcaact gcgctgtagc tacacgccgg
     5341 atcaaggttc cacgcgcatt gaacgcgtgc tggcctccgg tactggtgcg cgtattgtca
     5401 ccagccacga tgatgtctgt ttgattgagt ttcaggtgcc cgccagtcag gatttcaaac
     5461 tggcgcataa agagatcgac caaatcctga aacgcgcgca ggtacgcccg ctggcggttg
     5521 gcgtacataa cgatcgccag ttgctgcaat tttgctacac ctcagaagtg gccgacagtg
     5581 cgctgaaaat cctcgacgaa gcggggttac ctggcgaact gcgcctgcgt caggggctgg
     5641 cgctggtggc gatggtcggt gcaggcgtca cccgtaaccc gctgcattgc caccgcttct
     5701 ggcagcaact gaaaggccag ccggtcgaat ttacctggca gtccgatgac ggcatcagcc
     5761 tggtggcagt actgcgcacc ggcccgaccg aaagcctgat tcaggggctg catcagtccg
     5821 tcttccgcgc agaaaaacgc atcggcctgg tattgttcgg taagggcaat atcggttccc
     5881 gttggctgga actgttcgcc cgtgagcaga gcacgctttc ggcacgtacc gactttgaat
     5941 ttgtgctggc aggcgtggtg gacagccgcc gcagtctgtt gagctatgac gggctggacg
     6001 ccagccgcgc gttagccttc ttcaacgatg aagcggttga gcaggatgaa gagtcgttgt
     6061 tcctgtggat gcgcgcccat ccgtatgatg atttagtggt gctggacgtt accgccagcc
     6121 agcagcttgc tgatcagtat cttgatttcg ccagccacgg tttccacgtc atcagcgcca
     6181 acaaactggc gggagccagc gacagcaata aatatcgcca gatccacgac gccttcgaaa
     6241 aaaccgggcg tcactggctg tacaatgcca ccgtcggtgc gggcttaccg atcaaccaca
     6301 ccgtgcgcga tctgatcgac agcggcgata ctattttgtc gatcagcggg atcttctccg
     6361 gcacgctctc ctggttgttc ctgcaattcg acggtagcgt gccgtttacc gagctggtgg
     6421 atcaggcgtg gcagcagggc ttaaccgaac ctgacccgcg tgacgatctc tctggcaaag
     6481 acgtgatgcg caagctggtg attctggcgc gtgaagcagg ttacaacatc gaaccggatc
     6541 aggtacgcgt ggagtcgctg gtgcctgccc attgcgaagg cggcagcatc gaccatttct
     6601 ttgaaaatgg cgatgaactt aacgagcaga tggtgcaacg gctggaagcg gcccgcgaaa
     6661 tggggctggt gctgcgctac gtggcgcgtt tcgatgccaa tggcaaagcg cgtgtgggcg
     6721 tggaagcggt gcgtgaagac catccgctgg catcactgct gccgtgcgat aacgtctttg
     6781 ccatcgaaag ccgctggtat cgcgataatc cgctggtgat ccgtgggcct ggcgctgggc
     6841 gcgacgtcac cgccggggcg attcagtcgg atatcaaccg gctggcacag ttgttgtaat
     6901 ttcagaaatt taataatgcc cggtactcat gttttcgggt ttatggtttc taatgaaata
     6961 tattgaatta tcataggatt aggccggatt aagcgtttac gacgaatccg gcaagaagca
     7021 ataagtacat ggttagttta tatttgcagt ccggtttgct ttgcataccg gattttcttt
     7081 ttcttaccat cctgaagttt tttcatcttc cctgattttt cctcaccatc attggtcatt
     7141 tttcggttga cgcccttcgg cttttccttc atctttacat ctggacgtct aaacggatag
     7201 atgtgcacaa cacaacatat aactacaagc gattgatgag gtaaggtatg agcttttttc
     7261 acgccagcca gcgggatgcc ctgaatcaga gcctggcaga agtccagggg cagattaacg
     7321 tttcgttcga gtttttcccg ccgcgtacca gtgaaatgga gcagaccctg tggaactcca
     7381 tcgatcgcct tagcagcctg aaaccgaagt ttgtatcggt gacctatggc gcgaactccg
     7441 gcgagcgcga ccgtacgcac agcattatta aaggcattaa agatcgcact ggtctggaag
     7501 cggcaccgca tcttacttgc attgatgcga cgcccgacga gttgcgcacc attgcacgcg
     7561 actactggaa taacggtatt cgtcatatcg tggcgctgcg tggcgatctg ccgccgggaa
     7621 gtggtaagcc agaaatgtat gcttctgacc tggtgacgct attaaaagaa gtggcagatt
     7681 tcgatatctc cgtggcggcg tatccggaag ttcacccgga agcaaaaagc gctcaggcgg
     7741 atttgcttaa tctgaaacgc aaagtcgatg ccggagccaa ccgcgcgatt actcagttct
     7801 tcttcgatgt cgaaagctac ctgcgttttc gtgaccgctg tgtatcggcg ggcattgatg
     7861 tggaaattat tccgggaatt ttgccggtat ctaactttaa acaggcgaag aaatttgccg
     7921 atatgaccaa cgtgcgtatt ccggcgtgga tggcgcaaat gttcgacggc ctggatgatg
     7981 atgccgaaac ccgcaaactg gtgggcgcga atattgccat ggatatggtg aagattttaa
     8041 gccgtgaagg agtgaaagat ttccacttct atacgcttaa ccgcgctgaa atgagttacg
     8101 cgatttgcca tacgctgggt gttcgacctg gtttataaat agtgtggctt ttgtgaaaat
     8161 cacacagtga tcacaaattt taaacagagc acaaaatgct gcctcgaaat gagggcggga
     8221 aaataaggtt gtcagccttg ttttctccct cattacttga aggatatgaa gctaaaaccc
     8281 ttttttataa agcatttgtc cgaattcgga cataatcaaa aaagcttaat tacgatcaat
     8341 ttgacctaca tctctttaac caacaatatg taagatctca actatcgcat ccgtggatta
     8401 attcaattat aacttctctc taacgctgtg tatcgtaacg gtaacactgt agaggggagc
     8461 acattgatga gcacgtcaga cgatatccat aacacgacag ccactggcaa atgcccgttc
     8521 catcagggcg gtcacgacca gagcgcgggg gcgggcacaa ccactcgcga ctggtggcca
     8581 aatcaactcc gtgttgacct gttaaaccaa cattctaatc gttctaaccc gctgggtgag
     8641 gactttgact accgcaaaga attcagcaaa ttagattact acggcctgaa aaaagatctg
     8701 aaagccctgc tgacagaatc tcagccgtgg tggccagccg actggggcag ctacgccggt
     8761 ctgtttattc gtatggcctg gcacggcgca ggtacttacc gttcaatcga tggacgcggc
     8821 ggcgcgggtc gtggtcagca acgttttgca ccgctgaact cctggccgga taacgtaagc
     8881 ctcgataaag cgcgtcgcct gttgtggcca atcaaacaga aatatggtca gaaaatctcc
     8941 tgggccgacc tgtttatcct cgcaggtaac gtggcgctgg aaaactccgg cttccgtacc
     9001 ttcggttttg gtgccggtcg tgaagacgtc tgggaaccgg atctggatgt taactggggt
     9061 gatgaaaaag cctggctgac tcaccgtcat ccggaagcgc tggcgaaagc accgctgggt
     9121 gcaaccgaga tgggcctgat ttacgttaac ccggaaggcc cggatcacag cggcgaaccg
     9181 ctttctgcgg cagcagctat ccgcgcgacc ttcggcaaca tgggcatgaa cgacgaagaa
     9241 accgtggcgc tgattgcggg tggtcatacg ctgggtaaaa cccacggtgc cggtccgaca
     9301 tcaaacgtag gtcctgatcc agaagctgca ccgattgaag aacaaggttt aggctgggcg
     9361 agcacttacg gcagcggcgt tggcgcagat gccattacct ctggtctgga agttgtctgg
     9421 acccagacgc cgacccagtg gagcaactat ttcttcgaaa acctgttcaa atatgagtgg
     9481 gtacagaccc gcagtccggc tggcgcaatc cagttcgaag cggtagacgc accggaaatt
     9541 atcccggatc cattcgatcc gtcgaagaaa cgtaaaccga cgatgctggt gaccgacctg
     9601 acgctgcgtt ttgatcctga gttcgagaag atctctcgtc gtttcctcaa cgatccgcag
     9661 gcgttcaacg aagcctttgc ccgtgcctgg ttcaaactga cgcacaggga tatggggccg
     9721 aaatctcgct acatcgggcc ggaagtgccg aaagaagatc tgatctggca agatccgctg
     9781 ccgcagccga tctacaaccc gaccgagcag gacattatcg atctgaaatt cgcgattgcg
     9841 gattctggtc tgtctgttag tgagcgggta tcggtggcct gggcatctgc ttctaccttc
     9901 cgtggtggcg acaaacgcgg tggtgccaac ggtgcgcgtc tggcattaat gccgcagcgc
     9961 gactgggatg tgaacgccgc agccgttcgt gctctgcctg ttctggagaa aatccagaaa
    10021 gagtctggta aagcctcgct ggcggatatc atcgtgctgg ctggtgtggt tggtgttgag
    10081 aaagccgcaa gcgccgcagg tttgagcatt catgtaccgt ttgcgccggg ccgcgttgat
    10141 gcgcgtcagg atcagactga cattgagatg ttcgagctgc tggagccaat tgctgacggt
    10201 ttccgtaact atcgcgctcg tctggatgtt tccaccaccg agtcactgct gatcgacaaa
    10261 gcccagcaac tgaccctgac cgcgccggaa atgaccgcgc tggtgggcgg gatgcgcgta
    10321 ctgggtgcca acttcgatgg cagcaaaaac ggcgtcttca ctgaccgcgt tggcgtattg
    10381 agcaatgact tcttcgtgaa cttgctggat atgcgttacg agtggaaagc gaccgacgaa
    10441 tcgaaagagc tgtttgaagg ccgtgaccgc gaaaccggcg aagtgaaata cactgccagc
    10501 cgtgcggatc tggtatttgg ttctaactct gtcctgcgtg cggtggctga agtttacgcc
    10561 agcagcgatg ctcacgagaa gtttgttaaa gacttcgtgg cggcatgggt gaaagtgatg
    10621 aacctcgacc gtttcgacct gctgtaatct gaccttcttc agcgactgcc tttcaggcag
    10681 tcgctgaagt ttctttaccg gcgtatagtg tccacaggaa aactacacac tggatctctc
    10741 atgtctgccg caggaaagag caacccactg gcaatcagtg gcctggttgt gctcacactt
    10801 atctggagtt atagctggat tttcatgaag caagtcacca gctacatcgg tgccttcgac
    10861 tttaccgcct tacgctgcat tttcggcgct ctcgttttat tcatcgtcct tttattacgt
    10921 ggtcgcggaa tgcgcccgac accgtttaaa tacaccttag ccattgccct gttacaaacc
    10981 tgcgggatgg ttggcctggc gcagtgggcg ctggtcagcg gcggagcggg gaaggtggcg
    11041 atcctaagct ataccatgcc gttctgggtg gtgattttcg ccgcgttgtt tctcggtgaa
    11101 cgcctgcgac gtgggcaata tttcgcgata ctgattgccg ctttcggctt atttttggtg
    11161 ttgcagccgt ggcaactcga tttctcttcg atgaaaagtg ccatgctggc aatcctctcc
    11221 ggcgtcagtt ggggggcgag cgcgattgtt gctaaacgtc tgtatgcccg tcatccgcgc
    11281 gtggatttat tgtcgttaac atcctggcag atgctctatg cggcgctggt gatgagtgtg
    11341 gtcgctttac tggtgccgca acgtgaaatt gactggcagc ccaccgtgtt ctgggcgctg
    11401 gcctacagtg cgattctggc gacggcactg gcgtggagct tatggttgtt tgtattgaaa
    11461 aacttgcctg ccagtattgc cagcttaagc acactggccg ttcccgtttg cggcgtactc
    11521 ttttcctggt ggctgctcgg cgagaatccg ggggccgttg aaggtagcgg tattgtgctg
    11581 attgtgatgg cactggcgct ggtgagccgt aagaaaaaag aagccgttag tgtaaaaagg
    11641 atctgaattt tttcttcatg tggggcgatc gcttatttaa caaaataacg ataatgcccc
    11701 accatgcgcc agctaaacag cacatcttct tcctgcgcac ctgcgccaat gttatgtatc
    11761 accagcggcg taccgtcgcg ggcgaagcca tctgaaacca ccccaatatg tgccagcccg
    11821 ttatctaatc gccaggagac aatatcgccc gcttgatagt cactggggtt cttgctgatg
    11881 gggcgtgttt tatcgtggtg ggtaaaccag gtttccagat taggcacccg acggtgatcg
    11941 atgttgctgt ccgggcgctt taactgccat ttttgcgggt actcggcaaa attcttcgcc
    12001 atatcttcgt gcaccagttt ctgcaaatcg accttctggc tgcgcaatgc acggatcacc
    12061 acatcggaac ataccccgcg tttttgcggg acatcaccgc caggataggt aagctgcata
    12121 tacgccggat cgtaaaatag cgtgctgccg atttgctgtc tggcaccgtc tgcgatggca
    12181 aggttggtat tggcctggat ttgtaccacg gttggtggaa cggcgggaga ttttaaggag
    12241 tggctggtaa atgccgttag caggctgagc agcgccagtg aagctttcat ctggttcgtc
    12301 cgtgaaaata agagtcacag attcgacctt ccgggataac gattgttctg tagagaaaat
    12361 gtccagaagc ggataaaaaa ctcctgaaca acgtgggagt ttcagattac tggcacagag
    12421 tgccggatgc ggcgtgaacg ccttatctgt cctaaaaaaa aagaaaattc aatatattgc
    12481 agagattgcg taggcctgat aagcgtagcg catcaggcaa ttttgcgttc aaactcccgg
    12541 acaagccggg agtttggagt aggttattcc cactcttgca ggaaacgctg accgtactgg
    12601 tcggctacca gcagagcggc gtaaacctga tctggcgtcg cgccgccagg catgttgtgg
    12661 atggtttcac attctgcaca tgccgcttca gccacaattc gcattttcgc cgggacatct
    12721 tctttaatat ccagttgagc gagagttatt ggcaaaccta ccgcatggct aagtgcagct
    12781 acggtttcga tttcctcaac cggtgcgttt tccagaacca gctgcgtcag cgtaccaaat
    12841 gccacttttt caccgtgata atagtgatgc gcgtccggga tagcggtcag gccgttatgc
    12901 actgcgtgcg ccgcagccag accaccactt tcaaaaccaa caccgctcaa ataggtgttc
    12961 gcttcaatca cgcgctccag cgccggagtc actacatgct gttcggcagc aagcatcgct
    13021 ttttcgcctt cttccagcag ggtgttgtag cacagttcag ccagtgccag cgcagcctgg
    13081 gtgcacttgc cgcccgccat ggtggtcgcg ccgctacgag agcaggcacg cgcttcaaac
    13141 caggttgcca gcgcatcgcc gatacccgcc gctaacagac gtgcaggtgc gccagcgacg
    13201 attttggtgt cgacaatgac catattcggg ttatttggca acagcagata gcggtcaaac
    13261 tcaccctcat cggtgtagat aacagacaat gcgctgcacg gtgcatcggt agaggcgata
    13321 gtcggtgcga tcgctaccgg aacacccatg aaatgtgcca gtgctttggc tgtatcgagg
    13381 gtttttccgc caccgatacc gagaattgcg ccacactgcg cagtctccgc gatgccacgc
    13441 agacggtcga tctcattttg cgaacatcca ccgccaaacg gcgcaatttc tactaccagt
    13501 ccagcatctt taaagctttt ctcgacagtg gattgagcaa aacctaaaac aaatttgtca
    13561 cccaccacta accagcgttc tgccagcggc ttcaggtatt cgcccagacg attaatcaca
    13621 tcagcgccct ggatgtattt acccggtgat tgaataatgc ggtccataat tgctccttta
    13681 gagatgagta gtgccaaatg cggcattcca gtcgtgttcg aacttctcta tagctgactc
    13741 taccgcaggg gtgttgagca tttgttgcgc tacatctaag ggcagggtga tggattcaca
    13801 tcctgccagt aaacagtcca gcgcctgacg cggcgtttta aagctggctg ccagcaccat
    13861 gctttctggc gcgtgcattt ctaacagcgc ttgcagctcc tgaaccgtac gaatgccgtc
    13921 tccgccctgg gcatctacgc ggttaacata cggagcaacg tattttgccc ctgccagcgc
    13981 ggccagtaac ccttgtgcag cactgtacac tgcggtgcca agcgtagtaa taccctcttt
    14041 tttcagtatt ttaattgctg ccagaccttc ggaagtcacc gggattttca ccacaatacc
    14101 cggaatagcg tcgcgcaggc gcttcgcttc ttccaccatc ccctgcgcgt cgcggctcat
    14161 ggtctgagca aacagaatgc cctcatcacc aatcgctttt tgcagacgcg gcagcacttc
    14221 ccatatggac tccttgctgg cagcgataat gctcgggtta gttgtcaccc cggcaatggg
    14281 gaatatgcgt gccagacgtt cgacttctgc gacgttagcg gtgtccagat acagttccat
    14341 gatgtttcct tttacagttc cagttcatgt tgcagcaggc tggcgatagc gtcggcagag
    14401 gcggcgttaa ccagcgcgtt acggaattct tcgtgcatga tgcggcgagc gaggcgcgag
    14461 aaaatgcgca tgtgctgatc gcccgcagcg tgtttgttca gggttaacat gatgatgaat
    14521 tgcgcttcat catcacccca gcgcaccggc gcttgcagac gcgcaacgct gatggtggat
    14581 tgctcaatgt gttctgattt gctgtgtgga atggcaaaac taaagcccag accggtagag
    14641 aaaacggcct cgcgcgccca caagtcggct tccagtttac gcggatagcg gcagcgccct
    14701 gccagcagca ggttatcggt catgcctttg agcacttctt ctttgctgcg ccagtcgctt
    14761 tccagtgtga tgcactcggc ggtgaccagc ggtgcgtctt gttgggtcat gcggaattgc
    14821 gccagcaggt gctccacttc cagcgaagta cggcaggcca ttgcctggtt gagcaacttg
    14881 cggcactcac ggctatcaag ttgcgccatc cgcgctttcg ccgccggaat tgatggtgcg
    14941 ctcatgctga gttcatccag ccctaagccg accagcaacg gcagcacgga acctttcgct
    15001 cccagctcac cgcacagacc aatccattta ccctggcgat gcaccgcctg cacggcgtaa
    15061 tcgagcgccc gcaagaatgc cggattcagg ctgttgtagt gacgagtaac cttagcgtta
    15121 tcgcgatcca ctgccagcag atattgcgtc aggtcattac taccaatact aaagaaatca
    15181 atctcttcgc agcattgatc gatgatgaac atcaccgacg gcacttccag cataatgccg
    15241 agctggatct tctcatcaaa cggaatgtgt tcgttacgta gttgctgttt ggcttctgcc
    15301 agtttttctt tcacccataa gatctcttcc attgaggaga tcatcgggat catgattttc
    15361 aggctgccgt gagcggaggc gcgcaggatc gagcgtagtt gtgtggtaaa taacgacgcg
    15421 tattcttcat aaatacgcac ggcgcgataa ccgaggaacg ggtttgcctc tgcgggaatg
    15481 tttagataat caacgggttt gtcgccgcca atgtccatag tgcgcacaat aatgctgcgt
    15541 ccgttggcag actccagcgc ctgacaaaaa atgttgtaca gctcgctttc gcccggtgca
    15601 ctggtgcgat ccatatagag catttcagtg cggaacaaac caacgccttc cgcaccattg
    15661 ccgaatgcgg cctgcgcttc cacggagtga gcgatgttag cggcaatttc aatgcggata
    15721 ccgtcagcgg tacgggcttg ttgggtcagc cagacacgct gttgctcacg cagggcgtcc
    15781 tgtacgcggg cttcttgctg ataataacga gctacggctt ctcctggctc aaccacaatc
    15841 gccccggcgt tgccgtcgat ataaatcgtt tgatgctgcc acggagtgag ggcatcaata
    15901 tccacaccaa ccagcgtcgg aatgttgaac gaacgggcaa ggatcaccgt atgtgaggtg
    15961 gtgccgccac ttttcagcaa cagacctttg aggtgatttt tatccagttc gaggaactgg
    16021 ctgggggtca gttcatcagc catacaaatg gcggactgcg tcagtttgcc cggtgccggg
    16081 aagcgttgct caccgtagat ttgctggagt aactggaagc agacgtcgcg tacgtccagg
    16141 gcacgttctt gcaggtagct gctgctggaa cgggcgaact cttcgcaaaa gtgattcgca
    16201 ctggcgacaa tcgcttcggc gcagcttaat ccctcgctga cgcctgccag taagtgctga
    16261 cgtaaagatg tatcgccagc cagggatcgg tgagcttcca gtatcgcgct ggtagcaccg
    16321 tcgctatcca gcagacgaaa ctcaatgttt ttcagtacca gcgtcaggcc gttttccagt
    16381 gtggattgct cggcgtcaac gtctttggct gcgggaagat tactcagcgc attgagatct
    16441 aaagaagaga tcggcgtcag aatgccgcct gcactaccgc tgcacacggt gcgggcgcgg
    16501 ataatttgcg gattcagatt ggtcagtgaa accggcagtg gttccagttc gtcagattta
    16561 acttccgcca gcggcgcatc gcagtggggg aattcatcgc gcagccattg gcttaaacgc
    16621 tggtgcgctt cctgttcgtc ggccccggaa atcagtaact ggcagttatc gcccgccagc
    16681 gtatcggtgc caatcagcgc aagggcgctt ttggcgttgc ccttgcggtc agtgtgcagg
    16741 ttatgccact caatttgtga tgaaaaagta ttacacagcg tttcaacgtg gcttgccgga
    16801 cgcgcatgta cgccgttagg tagctcacaa ataaattcca caatcagggc cattgcctct
    16861 ctcccggtac ggttttctgt ttacaggata agagcgcacg gcaacggcct gccatgtgac
    16921 aaatctgcca aaagctggac aaatgtaatg taaccgtcaa tttgcgacgc gtctcacaag
    16981 acgccgtttt gcggcatgct tccggtttat cgcaagttat gaggcggatc gcatttttgt
    17041 actgatatta caaaagtcca gtaaatggcc tttttatcca ctgtttgctc cgcctgcgat
    17101 tgcctattgt ttcctccatc aaaaatatgg acatgggctt tcgcaagccc gggagcagct
    17161 tatgaatgag ttggtgcaga tcctgaaaaa tacccgtcag catttaatga cgggcgtttc
    17221 acacatgatt cccttcgtgg tatcgggcgg tattttgctg gcggtttccg tcatgttgta
    17281 tggcaaaggc gcagtgccgg atgccgtagc cgatccaaat ctgaaaaaac tgtttgatat
    17341 cggcgttgcg ggcttgacgc tgatggtgcc tttcctcgcc gcttacattg gttattccat
    17401 tgcagaacgt tctgcgctgg ctccatgcgc tatcggtacc tgggttggta acagctttgg
    17461 tgcgggcttc tttggtgcac tgatcgccgg gattatcggc ggcatcgtgg tgcattacct
    17521 gaagaaaatt ccggtgcata aagttctgcg ctcggtgatg cctatcttca ttattcctat
    17581 cgtcggcaca ctgattaccg caggcatcat gatgtggggc ttgggcgagc ctgtaggggc
    17641 gttgaccaac agcctgactc agtggcttca ggggatgcag cagggcagca ttgttatgct
    17701 ggcggtgatc atgggtctga tgctggcgtt cgatatgggc ggtccggtta acaaagtggc
    17761 ctatgccttc atgctgattt gcgttgctca gggtgtttat accgtggtgg ccatcgctgc
    17821 cgtgggtatt tgtatcccgc cgctggggat ggggctggcg acgctgattg gtcgtaaaaa
    17881 tttctccgca gaagagcgcg aaaccggcaa agcggcactg gtgatgggct gtgttggcgt
    17941 tactggaggg gcgattcctt tcgccgctgc cgatccgctg cgtgtcattc cttccatcat
    18001 ggtcggttca gtttgtggtg cagtaactgc ggcgctggtc ggtgcgcagt gctatgcagg
    18061 ctggggtggt ctgattgtgc tgccggtggt tgaaggcaag ctgggttata tcgcagcagt
    18121 ggctgtcgga gcagtggtga cggctgtttg tgtgaacgtg ctgaaaagtc tggcgcgtaa
    18181 aaatgggtct tcgactgatg aaaaagaaga cgacctggat ttggattttg aaattaatta
    18241 attgaggaac gagccatgac gaaaattatt gcagtaaccg catgtccttc aggtgttgcc
    18301 catacctata tggcagcaga ggcgctggaa agtgccgcga aagcgaaagg ctgggaagtg
    18361 aaagtagaaa cctaggggtc aattggtctg gaaaatgaac tgactgcgga agatgtggcg
    18421 agcgctgata tggtgattct gaccaaagat atcggcatca agtttgaaga gcgttttgct
    18481 ggcaagacca tcgtgcgcgt caacatcagc gatgcagtaa aacgtgctga cgccattatg
    18541 agcaaaattg aagctcacct ggcgcaaacc gcttaagttc ttctcccgct cgcaagggcg
    18601 ggttcgcttt cccacaggag ttcctcatga cgaatcgtat ctctcgcctc aaaactgcac
    18661 tgtttgccaa tacccgtgaa atctcgctgg agcgggcgct gctttatacc gccagccatc
    18721 ggcaaaccga aggcgaaccg gtgatattgc gccgggcgaa agcaacagcg tatatccttg
    18781 aacatgttga aatttcgatt cgtgatgaag aactgattgc cggtaaccgc accgtaaaac
    18841 cgcgcgccgg gattatgtcg ccggaaatgg acccttactg gctgctgaaa gagctggatc
    18901 aattcccgac gcgtccgcag gaccgctttg ctatcagcga agaagataaa cgtatctacc
    18961 gtgaagagtt gttcccgtac tgggaaaaac gttcgatgaa agatttcatc aacgggcaga
    19021 tgacagatga agtaaaagcc gcgaccaaca cgcagatttt cagcatcaac cagacggata
    19081 aaggccaggg gcacattatt attgattacc cacgcctgtt gaatcacggt ctgggtgagc
    19141 tggtggcaca gatgcagcaa cattgtcagc aacagccgga gaatcacttt tatcaggccg
    19201 cgttgttact gctggaagcc tcgcagaaac acattttgcg ttacgccgaa ctggcggaaa
    19261 cgatggcggc aaactgcaca gatgcacagc gtcgcgaaga actgctgact attgcggaga
    19321 tctctcgcca taacgccgaa cataagccgc agacgttctg gcaggcgtgc cagttattct
    19381 ggtacatgaa catcattctg caatacgaat ccaacgccag ttcgctgtct ttagggcgct
    19441 tcgaccagta tatgttgccg ttctatcagg catctttaac ccagggcgaa gatccggcgt
    19501 tcctgaaaga actgctcgaa tctttatggg tgaaatgcaa cgacatcgtg ctgttgcgct
    19561 ccaccagcag cgcgcgttat ttcgcaggtt tcccgaccgg ctataccgca ctgctcggcg
    19621 ggttaaccga gaacggacgt agcgcggtga acgtgctttc gttcctttgc cttgacgcct
    19681 atcaaagcgt gcaattaccg caaccgaacc tcggcgtgcg cactaacgcc ttgatcgaca
    19741 cgccgttcct gatgaaaacc gccgaaacca ttcgcctcgg caccggtatt ccgcaaatct
    19801 ttaacgatga agtggtggta ccagcgttcc tcaaccgtgg cgtttcgctg gaagatgcgc
    19861 gcgactattc cgtagtgggc tgtgtggaat tatctattcc cggcagaacc tacggcttgc
    19921 atgacatcgc gatgtttaat ctgctgaaag tgatggaaat ctgcctgcat gaaaatgaag
    19981 gcaatgctgc gctgacttat gaaggtttac tggagcagat ccgcgccaag atcagccact
    20041 acatcaccct gatggttgag ggcagcaata tttgtgatat cggccatcgc gactgggcac
    20101 ctgtaccgct gctctcgtct tttatcagcg attgtctgga aaaaggtcgc gatattaccg
    20161 atggcggcgc gcgttataac ttctccggcg tacaggggat cggtatcgcc aacctgagcg
    20221 attctctcca tgcgttgaaa gggatggttt ttgagcaaca gcgtttaagt tttgacgaat
    20281 tgctgtcggt attaaaagcc aactttgcaa cgccagaagg cgaaaaagtc cgtgctcgct
    20341 taattaaccg cttcgagaaa tacggtaacg atatcgacga ggtggataac atcagcgccg
    20401 aactgttacg ccactactgc aaagaagtgg aaaaatacca gaacccgcgc gcggttactt
    20461 cacgccggga tcgtataccg tttctgctca cgtcccgttg ggatcggtgg ttggcgcgac
    20521 gccagacggt cgttttgccg gggaacaact ggcagacggc ggcttgtcac ctatgctggg
    20581 ccaggacgca caagggccaa cagcggtact gaagtcagtc agtaagctcg ataatacgct
    20641 gctgtctaac ggtacgttgc tgaacgtgaa attcactccg gcgaccctgg aaggtgaagc
    20701 gggattacgc aaactggccg acttcttacg ggcgtttacc cagcttaagt tgcagcatat
    20761 tcagtttaac gtggtgaacg ccgacacgtt gcgggaagcg caacagcgcc cacaagatta
    20821 tgccgggctg gtggtgcgcg ttgccggata cagcgccttc tttgtcgaac tgtcgaagga
    20881 gatccaggat gacatcatcc gccggacagc gcatcagctg taacgttgtg gaaacgcgcc
    20941 gcgatgatgt ggcgcgcatt ttcaacattc agcgttattc actgaatgac ggcgagggca
    21001 ttcgtacggt ggtctttttt aaaggctgtc cgcatctttg cccgtggtgt gctaatccgg
    21061 agtcgatctc cggcaaaatc cagacggtac gcagagaggc gaaatgtctg cactgtgcga
    21121 aatgtttgcg tgatgcggat gaatgcccct ccggggcgtt tgaacggatt ggtcgcgata
    21181 tcagccttga cgctctggaa cgggaagtga tgaaagatga cattttcttt cgcacgtccg
    21241 gcggcggcgt cacgctttct ggcggcgaag tgttaatgca ggcggagttt gctacccgtt
    21301 ttttacagcg actgcggctg tggggtgtgt catgcgccat tgaaactgcc ggagacgcgc
    21361 cagcaagcaa gctgttaccg ctggcgaaat tgtgcgatga agtgctgttc gatttaaaaa
    21421 ttatggacgc gactcaggcg cgggatgtgg tgaagatgaa cctgccacgc gtgctggaga
    21481 atctgcgttt gctggtgagt gagggtgtca acgtgatccc gcgtttaccg ttgatccctg
    21541 gtttcacgct cagcagggag aatatgcagc aggcgctgga tgtactgatc ccgctgaaaa
    21601 tcaggcagat ccatctgtta ccgtttcatc agtacggcga accgaaatac cgcctgctgg
    21661 ggaaaacatg gtcgatgaaa gaggtgcctg cgccgtcgtc agccgatgtg acaacgatgc
    21721 gcgaaatggc agaacgggcc ggatttcagg ttaccgtggg aggttaaaat ggcatacctg
    21781 gtggcagtaa ccgcctgcgt aagcggcgtg gcgcatactt atatggcggc ggaacggctg
    21841 gaaaagttgt gccagttaga gaagtggggc gtcagcattg aaactcaggg cgcgctggga
    21901 acggagaatc gtttagcgga cgaggatatc cgtcgggcgg atgttgctct gttgattacg
    21961 gatatcgagc ttgccggtgc cgagcgattt gaacattgcc gctatgtgca atgcagcatc
    22021 tacgcattcc tgcgtgagcc gcagcgggta atgagcgcgg tgcgcaaagt gctttctgcg
    22081 ccgcagcaaa cccatcttat tctggagtag tcggtttttc tgtcagttgg ctgtggtact
    22141 gccggcgata ttccgacggc gagcgttcgg tgtttttacg aaacagacgg cagaagtagt
    22201 tgctgtcgac aaagccgcag gcgtgcgcca cttcttttac cttcaggtca tagcctttca
    22261 ataacgtctt agcgtgctcc agtcgcgtgt gattcaggta ttcgttaaaa ccaatggccc
    22321 ccgttttttg aaacaggtgc gagagatagt ttggtgaaat atagaatgcc tgtgcgacag
    22381 attcgcgggt aagcgcggag gcatagcgtt cgtcgatata atcgcgaata gcttcaaata
    22441 gtgcctggct gcgtgaggcg gtctggattt ggctgccgag caaatcgcgg cagtggctaa
    22501 gcaggctggc tacgataagc cttgccgttt gctgctcctg cggctgcatt tgcatttcat
    22561 tgagcgtttg tagcagaaaa gaacctatac gtgggccgcg tcgggcgacg tgttgcttcg
    22621 ccagattttg atattgtttg ccatcccatt gcacgacgct gaagccgagt tgctgtttgc
    22681 caaacagcac gctaaaggtg gtagcgggtg ttttccattg tgggaaattc cagccaccag
    22741 cggcaacgta cagcacatcg ccgggaacta acgttgctcc agcgccggta tcaacaaact
    22801 caccttccag cacaatttcc agccgtggaa aatcgacctg atacgcaaga tcgggcaccg
    22861 ggccgttact gctggcaaag taaatctggc gcagggacag cgggccgttg atcaggcggg
    22921 agagcagata gctgacgccg tgatacatgt tattccctta ggtatgtaga cgggaaaaca
    22981 gtaactggca ttattaaaaa aggccacctg aaaagtgtgg cctgagggga gttcgatgta
    23041 aacattgcgg cagttgtatt gccggatgtg gcgtaaacgc cttatccagc ctaccgccgc
    23101 gatctgtagg ccggataaga cgcgtcaagc gtcgcatccg gcaaatagcg cctggctgat
    23161 aattactgat tacctacctg gtcgccatac ggcagagtgt cgtaatcgat cagcgcgttt
    23221 ttcttgtacg ggttaccaat ccagcgggta gtttctttga actgcggatt caccactgaa
    23281 cgggttggat cgtaaccgtc gtaagataag cccgccaaat ctgaccaggt gtggatcagt
    23341 tccgccaggc tgtatttacg atcgacatcc tgtgagaaat cacggggatg agtcgcttgc
    23401 catttttccg acgtccacag caggaacgga atggtgtaca tgtggcgcgt cgggttgtct
    23461 tcgttacgcc cctgagtttt gtgcggcggc gtgtcgtaaa cctcttcacc gtggtcagag
    23521 aagtaaacca ggaatccgtt cgggtctgcc gctttaaagt ctttaatcag gctggcaacc
    23581 acatgatcgt tatacaggtt agcgttgtca taatcgttat atgattccag ctcttctgcg
    23641 cttaatcctg gcggaacatg atcggtattg ccatcaaact tgccctgatt ttccgggtag
    23701 cggtatttgt atttgatatg cgtacccagc agatgaacga tgatcagttt cttcggcgca
    23761 gggtcattca gcacatcctg gaacggcttc agcacgttgg tgtcgtattc acgcgcactc
    23821 tgcgtacgtt gctggttcat gtagtactgc ttgtcggtct ggcgcgaaaa taccgtcagc
    23881 atggtattgc gggcggtcat cgtctgctgg ttggtgatcc agaaggtttt ataacccgcc
    23941 tgtttcatca tgttcatcag cgacggctgc gtcagataca gatccgggtt cttttcattg
    24001 gcaaaggtca gcgcctgttg caggatttca atggtgtacg gacgagacgt aactacgtta
    24061 ttaaacacgg tcagattcgg atcggtttta tgcagcgcat ccagctccgg cgtggtttca
    24121 cgcggataac cgtacagact catgcgtccg cgctgggtcg actcgccaat caccagcact
    24181 aaagtgcgcg gttcgttacc cgattcatct ttgaaattag ccagtggcgg caaggcatta
    24241 ttttcattca gtaactttgt tagcgagttt agttgctgac gatactgata atagccggtc
    24301 aggaattgcc acggtgcggc aggctccata cgcgaggcca ggttatccaa cgttttctca
    24361 aacggcttgt ttttgataaa cgtattcatg gcgatcggat gcagaatcaa gccataaagc
    24421 agggcaaaag agacaacata acgccacggc tttggaatat agaccgggcg caggcgtgtc
    24481 cacagcagaa ctgccaccgc cgtataggcc agcgcgataa gcacaatttt caggctgaaa
    24541 tactggctta aatactcgct ggcttcgttg gtgttggttt cgaacatcac aaacagaacg
    24601 ctctgcgaga actcctgccc gtagatgacg tagtagcata gcgccgccag agaggccgcc
    24661 catagcacca cgccgattac tgcggcaata attttaatcc gcttcggaaa gaggaatacc
    24721 gggatcaacc acagcgagct gaataacagc gagtcgcgaa tgccgttggt gccactataa
    24781 ccactgatgt aaataatggc ctgtagcaga gtagagaaaa accaaaagta gagcagtgcc
    24841 caacccaggg ctttccagct aaaaaggggt ttagcctgga cttctgtgga atgcatagta
    24901 agaacctgtc ttgaaaaaaa tatcgccgaa tgtaacgaca attccttaag gatatctgaa
    24961 ggtatattca gaatttgaat aaaatgcaga cagaaatata ttgaaaacga gggtgttaga
    25021 acagaagtat ttcagaaaac cctcgcgcaa aagcacgagg gtttgcagaa gaggaagatt
    25081 agccggtatt acgcatacct gccgcaatcc cggcaatagt gaccattaac gcttgttcga
    25141 cgcgaggatc cggttcctgg ccttcttttt ctgcctggcg ggagcggtgc agcaactcgg
    25201 cctgcaatac gttcagcggg tcggtgtaaa tattccgtag ctgaatagac tctgcaatcc
    25261 acggcagatc ggccatcaga tgggaatcgt tggcaatcgc cagcaccact ttgatgtctt
    25321 cttcttgcag gttgcgtaac tctttaccta acggccacag tgctttgtct accagacgtt
    25381 gatcatagta ttccgccaac cacaggtctg ctttggcgaa gaccatctcc agcatgccga
    25441 gacgcgtcga gaagaatggc caatcgcggc acatggcttc cagctcgctc tgtttgccat
    25501 cttcgaccac tttttgcagc gccgtacctg cacccagcca ggcggggagc atcaggcggt
    25561 tttgcgtcca ggcaaaaatc cacggtatgg cgcgcagcga ctcgacgccg ccggttgggc
    25621 gacgtttcgc cggacgtgaa cccaacggca gtttgcccaa ttcttgttcc ggcgtagcgg
    25681 agcggaagta aggcacaaaa tctttgtttt cacgtacgta gccacggtag agatcgcagg
    25741 agatgactga cagttcatcc ataatgcgac gccagctctc tttcggctca ggtggtggca
    25801 gcaggttggc ttccagaatc gccccggtgt aaagcgacag gctgctgacg gtgatttctg
    25861 gcagaccata tttaaagcgg atcatctcgc cctgttcagt cacgcgcagg ccacctttca
    25921 ggcttcctgg cggttgtgac agcagcgccg catgggcagg tgcgccgccg cgaccaatgg
    25981 aaccgccgcg accgtggaac aacgtcagct caatacccgc tttttcgcag gttttgatta
    26041 atgcatcctg tgcctgatat tgcgcccagg aagctgccat cacgcccgca tcttttgctg
    26101 agtcggaata gccaatcatc accatctgtt tgccctgaat caggccgcga taccagtcga
    26161 tattgagcag ctgggtcatg acatcgttgg cgttgttcag gtcatcgagg gtttcaaaca
    26221 gcggagcaac cggcatcgca aacccgatac ccgcttcttt cagcagcagg tggacagcca
    26281 gtacgtcgga cggcgttttc gccatcgaga tcacgtaggc ggcaatggag ccttgcggcg
    26341 cttcggcaat cacctggcag gtatcgagca cttcgcgcgt ttcggcgctt ggttgccagt
    26401 tgcgcggcag aagcggacgt ttggagttca gttcgcggat caggaacgcc tgtttgtcgg
    26461 cctctgacca gctttcgtag tcgccgatac cgaggtagcg ggtcagctcg cccagcgctt
    26521 cggtatggcg ggtgctctcc tgacggatat caatacggac cagcggtacg ccgaaacatt
    26581 tcacgcggcg cagggtgtcg agcagatcgc cgttggcgat aatacccatg ccacacgcct
    26641 gaagtgactg gtagcaagcg tagagcggtt cccacagttc ttcgttttgt gtcagcagcc
    26701 cttctggttt tggcagttct tcgcctttca ggcgcgcttc cagccatgcc tgtgtcgcca
    26761 tcaggcgaga acgcaggttt ttcatcagat agcgatacgg ttctgcggca ccttcttcgc
    26821 caaccagcgc cagcagttca ggggtcgctt caaccatcga cagttcagaa accagaacct
    26881 gaatatcttt caggaacaga tcggtggctt tccagcgact gagtagcagg acgtggcggg
    26941 tgatatcggc agtgacgttc gggttgccgt cgcggtcacc gcccatccac gaagtaaaac
    27001 ggaccggaac aaattcgacg ggcagtttgt agccgaggtt ctcttccagt tgttcgttca
    27061 gttcgcgcaa gtaattcggt acgccttgcc acaggctgtt ttctactacg gcaaagcccc
    27121 atttggcttc atctaccggg cttggacgca gcttacggat ttcatcagta tgccatgact
    27181 gggcgattaa ctggcgcagg cgacgcatca gctggttgtg ttcgtagtca gcgatatctt
    27241 tgttatcgag ctgttttaag caggcgttca cttccaccat tttgtggatc agtgtacgac
    27301 gggtaatttc ggttgggtga gccgtgagga ccagttccag cgacagcgat tccactgctt
    27361 ttttgatggt gtcttcgctc agttccggct ggtttttcag tttgcgcagg gtgcgggcga
    27421 tcacttccgg gttgctggca gcttcgcctt tcggcgaaat gctgtggtat tgctcggcgg
    27481 tgttggccag gttcaggaac tgactaaacg cacgcgcaac gggcagcagc tcgtcgttcg
    27541 acaaattttg taaggtggtg agcaactcct ggcggttagc atcattgcca gcgcgtgaag
    27601 atttcgacaa cttacggata gtttctacgc gttcaagaat gtgttctccc aacgcatcct
    27661 tgatggtttc tcccagcact ttgccgagca tactgacatt actacgcaat gcggaatatt
    27721 gttcgttcat attaccccag acaccccatc ttatcgtttg atagccctgt atccttcacg
    27781 tcgcattggc gcgaatatgc tcgggctttg cttttcgtcg tcttttataa agccacgtaa
    27841 aagcggtgac gtcaaatgct gcgaaatcgc ttcagcaaac gaataaatag caggaattta
    27901 cgccattaaa ttcacgacgc tttaaataag cgtaacttat ggaaatgtta aaaaatcgcc
    27961 atacataaca ccaaaggtgt aggtcggata agatgcgcaa gcatcgcatc cgacattatt
    28021 gcggcaccgg agtttggcaa cggtgccgga tgcggcgcga gcgccttatc cggcctacgt
    28081 tttaatgcca gcaaaaatgg tgaattacct ggataatcaa ctcacgggtg ggtttaataa
    28141 accgcgtctc cagatattca tcaggctgat gagcttgatt gatggaaccc ggaccgagca
    28201 ccagcgtcgg gcataacgtt tgaataaacg gcgcttcggt acagtaattc accacttcgg
    28261 tttttgctcc gagcaatttc tcaaccactt caaccagttg atgattcggt gggcattcat
    28321 agccagggat cggcggatgc agctcgtcga ccgtcagacg acccggccag cgttcgctca
    28381 ccggagccaa tgcatcgttg agcaaaccat taagttcatt gagtgtcatg ctaggcagcg
    28441 gacgaatatc catatgcaac tcacagcaag cgcaaatacg gttagaagcg tcgccaccgt
    28501 gaatatgccc gaggttgagc gtagggtatg gcacggtaaa tgcttcgtag tgataacgtt
    28561 ctttcaggtt atcgcgcaat tgcaaaatat gcccgatggc gtcgtgcatt agttcgatag
    28621 cgttaactcc gcgtgctgga tcgctggagt gccccgactg gccctgaata cggatggcgt
    28681 tagagatatg acctttatgt gcgcgtaccg gttgtagtga cgtcggttcg ccaatgatgg
    28741 cgcaatccgg gcgcagggcg gtagtttcgg caaaataacg cgctccggcc atactggttt
    28801 cttcatcagc agtcgccaga atgtagagcg gttttttcag tttcgtgacg tcgacatcgc
    28861 gtagcgtatc aaggataaac gcaaaaaagc ctttcatgtc ggcggtgcct aagccgtaaa
    28921 gcttgccgtc atgctccgtc agtgtaaacg gatcgcgcgt ccagcgaccg tcatcaaatg
    28981 gcaccgtatc ggtatgcccc gccagcaaca agccgccagc cccctgtccg atacttgcca
    29041 gcatattgaa tttgttgcga gttcctggca caggctgcac ttccacattg aagcctaaat
    29101 ctttaaacca gtccgccagc agagtgatta aatctgcatt gctttgatcg agtgcctctt
    29161 ccgtggcgct tattgaaggt gtggcaatca gagcacggta aatctcgata aatggcggta
    29221 atttgttttt cattgttgac acacctctgg tcatgatagt atcaatattc atgcagtatt
    29281 tatgaataaa aatacactaa cgttgagcgt aataaaaccc accagccgta aggtgaatgt
    29341 tttacgttta acctggcaac cagacataag aaggtgaata gccccgatgt tgaatacgct
    29401 gattgtgggt gccagcggct acgctggcgc agagctagtg acctatgtaa atcgccatcc
    29461 gcatatgaac ataaccgctt tgactgtttc agcgcaaagc aatgatgcgg gaaagttaat
    29521 ctccgatttg catccgcagc taaaaggcat cgttgagctg ccgttgcagc cgatgtcgga
    29581 tatcagcgag tttagcccag gggtggacgt agtgtttctc gccaccgccc atgaagttag
    29641 ccacgattta gcgccgcagt ttcttgaagc gggctgcgtg gtgttcgacc tttccggtgc
    29701 gtttcgtgtt aacgacgcca ccttctatga aaaatattac ggctttaccc atcaataccc
    29761 ggaactgctg gaacaggcag cctacggtct ggcggagtgg tgcggtaata aattaaaaga
    29821 agcgaatttg attgcggtgc caggctgtta tccgacggcg gcacagctgg cgctgaaacc
    29881 gttgattgat gccgatcttc ttgacctcaa tcagtggccg gtgatcaacg ccaccagcgg
    29941 cgtgagcggt gcaggacgta aggcagcgat ttcaaatagt ttttgtgaag ttagcctgca
    30001 accgtatggc gtctttactc atcgccatca accagagatc gccacacacc tcggtgctga
    30061 cgttatcttc accccacatc tgggcaattt cccgcgcggc attctcgaaa ccattacctg
    30121 ccgcctgaaa tcgggtgtga cccaggcgca agtcgcgcaa gtgttacagc aggcgtatgc
    30181 ccataaaccg ctggtgcggc tgtatgacaa aggcgttccg gcgctgaaaa atgtcgttgg
    30241 gctgccattt tgcgatatcg ggtttgccgt tcagggcgag catctgatta ttgtggcgac
    30301 cgaagacaac ttactgaaag gcgcggcggc acaagcggta cagtgcgcca atattcgttt
    30361 cggctatgcg gaaacgcagt ctcttattta agggtgcaat gatgaatcca ttaattatca
    30421 aactgggcgg cgtactgctg gatagtgaag aggcgctgga acgtctgttt agcacactgg
    30481 tgaattatcg tgagtcacat cagcgtccgc tggtgattgt gcacggcggc ggttgcgtgg
    30541 tggatgagct gatgaaaggg ctgaatctgc cggtgaaaaa gaaaaacggc ctgcgggtga
    30601 cgcctgctga tcagatagac attatcaccg gagcactggc gggaacggca aataaaaccc
    30661 tgttggcatg ggcgaagaaa catcagattg cggccgtagg tttgtttctc ggtgacggcg
    30721 acagcgtcaa agtgacccag cttgatgaag agttaggtca tgttggactg gcgcagccag
    30781 gttcgcctaa gcttatcaac tccttgctgg agaacggtta tctgccggtg gtcagctcca
    30841 ttggcgtaac agacgaaggg caactgatga acgtcaatgc cgaccaggcg gcaacggcgc
    30901 tggcggcaac gctgggcgcg gatctgattt tgctctccga cgtcagcggc attctcgacg
    30961 gcaaagggca acgcattgcc gaaatgaccg ccgcgaaagc agaacaactg attgagcagg
    31021 gcattattac tgacggcatg atagtgaaag tgaacgcggc gctggatgcg gcccgcacgc
    31081 tgggccgtcc ggtagatatc gcctcctggc gtcatgcgga gcagcttccg gcactgttta
    31141 acggtatgcc gatgggtacg cggattttag cttaagtttt gttggccgga ggcgcagctt
    31201 tccggcattg aatttcaaaa taaggaaaca gagttatggc actttggggc gggcgtttta
    31261 cccaggcagc agatcaacgg ttcaaacaat tcaacgactc actgcgcttt gattaccgtc
    31321 tggcggagca ggatattgtt ggctctgtgg cctggtccaa agccctggtc acggtaggcg
    31381 tgttaaccgc agaagagcag gcgcaactgg aagaggcgct gagcgtgctg ctggaagatg
    31441 ttcgcgccag gccacaacaa atccttgaaa gcgacgccga agatatccat agctgggtgg
    31501 aaggcaaact gatcgacaaa gtgggccagt taggcaaaaa gctgcatacc gggcgtagtc
    31561 gtaacgatca ggtagcgact gacctgaaac tgtggtgcaa agataccgtt agcgagttac
    31621 tgacggctaa ccgacagctg caatcggcgc tggtggaaac cgcacaaaac aatcaggacg
    31681 cggtcatgcc aggttacact cacctgcaac gcgcccagcc ggtgacgttc gcgcactggt
    31741 gcctggccta tgttgagatg ctggcgcgtg atgaaagccg tttgcaggat gcgcttaagc
    31801 gtctggatgt cagcccatta ggctgtggcg cgctggcggg aacggcctat gaaatcgacc
    31861 gtgaacagtt agcaggctgg ctgggctttg cttcggcgac ccgtaacagt ctggacagcg
    31921 tttctgaccg tgaccatgtg ttggaactgc tgtcggctgc cgctatcggc atggtgcatc
    31981 tgtcgcgttt tgctgaagat ctgattttct ttaacaccgg cgaagcgggg tttgtggagc
    32041 tttctgaccg cgtgacttcc ggttcatcat taatgccgca gaagaaaaac ccggatgcgc
    32101 tggagctgat tcgcggtaaa tgcggtcggg tgcagggtgc gttaaccggc atgatgatga
    32161 cgctgaaagg tttgccgctg gcttacaaca aagatatgca ggaagacaaa gaaggtctgt
    32221 tcgacgcgct cgatacctgg ctggactgcc tgcatatggc ggcgctggtg ctggacggca
    32281 ttcaggtgaa acgtccacgt tgccaggaag cggctcagca gggttacgcc aacgccaccg
    32341 aactggcgga ttacctggtg gcgaaaggcg tacctttccg cgaggcgcac catatcgtgg
    32401 gtgaagcggt ggtggaagcc attcgtcagg gcaaaccgct ggaagatctg ccgctcgacg
    32461 agttgcagaa attcagcccg gtgattggcg aagatgtcta tccgattttg tcgctgcaat
    32521 cgtgcctcga caagcgtgcg gcaaaaggcg gcgtctcacc gcagcaggtg gcccaagcga
    32581 ttgcttttgc gcaggcgcgg ttggggtaag aacatttata tgtacaaatt tgagcctggc
    32641 ttatcgccgg gcttttttat ggcaaaaaaa agcggatcct ggagatccgc aaaagttcac
    32701 gttggcttta gttattcgag ttgagaaact ctcgaaacgg gcagtgactt caagggttaa
    32761 aagaggtgcc gctccgtttc tgtgagcaat tatcagtcag aatgcttgat agggataatc
    32821 gttcattgct attctaccta tcgccatgaa ctatcgtggc gatggaggat ggataatgaa
    32881 tattcgtgat cttgagtacc tggtggcatt ggctgaacac cgccattttc ggcgtgcggc
    32941 agattcctgc cacgttagcc agccgacgct tagcgggcaa attcgtaagc tggaagatga
    33001 gctgggcgtg atgttgctgg agcggaccag ccgtaaagtg ttgttcaccc aggcgggaat
    33061 gctgctggtg gatcaggcgc gtaccgtgct gcgtgaggtg aaagtcctta aagagatggc
    33121 aagccagcag ggcgagacga tgtccggacc gctgcatatt ggtttgattc ccacggttgg
    33181 accgtacctg ctaccgcata ttatcccgat gctgcaccag acctttccaa agctggaaat
    33241 gtatctgcat gaagcacaga cccaccagtt actggcgcaa ctggacagtg gcaaactcga
    33301 ttgcgtgatc ctcgcgctgg tgaaagagag cgaagcattc attgaagtgc cgttgtttga
    33361 tgagccaatg ttgctggcta tctatgaaga tcacccgtgg gcgaaccgcg aatgcgtacc
    33421 gatagccgat ctggcagggg aaaaactgct gatgctggaa gatggtcact gtttgcgcga
    33481 tcaggcaatg ggtttctgtt ttgaagccgg ggcggatgaa gatacacact tccgcgcgac
    33541 cagcctggag acgctgcgca acatggtggc ggcaggtagc gggatcactt tactgccagc
    33601 gctggctgtg ccgccggagc gcaaacgcga tggggttgtt tatctgccgt gcattaagcc
    33661 ggaaccacgc cgcactattg gcctggttta tcgtcctggc tcaccgctgc gcagccgcta
    33721 tgagcagctg gcagaggcca tccgagcaag aatggatggc tatttcgata aagtgttaaa
    33781 acaggcggtt taaaccgttt aacgcagcta cccgataggc ttccgccatg gtcgggtagt
    33841 taaaggtggt gttgacgaag tactcaatag tgttgccgcc acctttctgt tccataatcg
    33901 cctgaccgat atgaataatt tcggcagcgc gctcgccaaa gcagtgaata cccagaatct
    33961 cttttgtttc ccgatggaac aaaattttca gcgtgcccac gttcatgccg acgatttgtg
    34021 cgcgtgccag atgtttaaac tgggcgcggc ccacttcata tggcactttc atcgcggtca
    34081 gctgctgttc ggttttgccc acagagctga tttccgggat ggtgtaaatg ccggtaggga
    34141 tatcttcaat cagatgcgcg gtggcttcgc cttttaccag cgcctgcgcg gcaatgcgcc
    34201 cctggtcata ggccgctgat gccaggctcg gataaccaat cacgtcgccc accgcgtaaa
    34261 cgtgcggctg tgcggtctga tacatgctgt tgaccttcag ctgtccgcga ctgtcagttt
    34321 ccagcccaat gttctgtaac gccagcgaat cggtattacc ggtacgaccg ttggcataga
    34381 gcaggcagtc agctttcagt tttttacccg acttcagatg catgatcaca ccgtcgtcac
    34441 agccttcgat cttctcgtac tcttcgttgt gacgaatcac tacgccactg ttccagaagt
    34501 gataggagag agaatctgac atctcttgat cgagaaatgc cagcaggcga tcgcgggtgt
    34561 tgatcagatc cacttttaca tccataccgc ggaagatcga cgcatattca cagccgatca
    34621 ctccagcacc atagataagt acatggcgcg gttcgtggtg catgctaaga attgagtcgc
    34681 tgtcgtaaat gcgtggatgg gtgaaatcaa catctgttgg atgatatgga cgagagccgc
    34741 aggcaataac aaatttttca gcggttagtg tttcgacgct gccgtccggg caatccagcg
    34801 ccaacgtatg ctcgtcaaca aagcgagcgt tcccctgcaa tatttcacag tgattacgtt
    34861 cgtaaaatcc ctgacgcatg cgcgtttgtt gattaatcac gttatcggca tggttaagga
    34921 tatcggcaaa agaagagcgg agcagtcgcg aatggtcgct gtatagtggg ttttgattga
    34981 attctataat gcggctgacg gcgtgacgga gagctttcga cgggatggtg ccccagtggg
    35041 tgcaaccgcc gccaacattt tgataacgct cgataactgc gacgcgcaca ccttgcttaa
    35101 ccaggcccat tgcagcgcct tcgccgccgg ggccggaacc tattactatg gcatcgtaat
    35161 cgtaggaatg tggcatggta gggcttacct gttcttatac ataaaagcaa cagaatggta
    35221 acattctatc gcgggtaagc caattgatcc ccgtcattta tctggctata tcctgagcgg
    35281 cctttgcttt gtctgtttct tacttttgcc ctgacgtttt attggatttt tatcgacgat
    35341 actctccgtt taagcggcag gtttccgctg tacgtaaaag aaccggccag taaatatgtt
    35401 ttattgcgtt accgttcatt cacaatactg gagcaatcca gtatgttcat tctctggtat
    35461 agtgccagca gtacttttgg caaggattca gacatcgtga tgggcataag agcgcaacaa
    35521 aaagaaaaaa cccgccgttc gctggtggaa gccgcattta gccaattaag tgctgaacgc
    35581 agcttcgcca gcctgagttt gcgtgaagtg gcgcgtgaag cgggcattgc tcccacctct
    35641 ttttatcggc atttccgcga cgtagacgaa ctgggtctga ccatggttga tgagagcggt
    35701 ttaatgctac gccaactcat gcgccaggcg cgtcagcgta tcgccaaagg cgggagtgtg
    35761 atccgcacct cggtctccac atttatggag ttcatcggta ataatcctaa cgccttccgg
    35821 ttattattgc gggaacgctc cggcacctcc gctgcgtttc gtgccgccgt tgcgcgtgaa
    35881 attcagcact tcattgcgga acttgcggac tatctggaac tcgaaaacca tatgccgcgt
    35941 gcgtttactg aagcgcaagc cgaagcaatg gtgacaattg tcttcagtgc gggtgccgag
    36001 gcgttggacg tcggcgtcga acaacgtcgg caattagaag agcgactggt actgcaactg
    36061 cgaatgattt cgaaaggggc ttattactgg tatcgccgtg aacaagagaa aaccgcaatt
    36121 attccgggaa atgtgaagga cgagtaatga aacaagcaaa tcaagataga ggtacgctgc
    36181 tgctggcgtt agttgctggc ttatcgatta atggtacttt cgcagcgctg tttagctcca
    36241 ttgtgccatt ttctgtattc ccgattattt ccctggtgct gacggtttac tgcctgcatc
    36301 aacgttatct taatcgcact atgccggtag gcttgccggg tctggcagct gcctgtttta
    36361 ttctcggcgt actgctgtac agcacggtag ttcgtgcgga atatccggat atcggctcta
    36421 acttcttccc ggcagtactc tccgtcatta tggtgttctg gattggcgcg aagatgcgta
    36481 accgtaagca ggaagttgct gagtaatcgg tattatgccg gatgctggcg catccggcat
    36541 gggttttact tcgcggtcag taatacgccg cactccatat ggtgcgtgta ggggaactga
    36601 tcaaacagag ccagacgttc gaccttgtgc gtctggctta atgtttccag attcttgcat
    36661 aacgtttccg ggttacagga gatgtacaaa atacacggat acgcctgcac cattttctcg
    36721 gtttcactgt ccagaccgct gcgcggtggg tcgacaaaaa tggtttcgca ctgataactc
    36781 tttaagtcga tcccttgcag gcggttaaac tcgcgcacac cattcatcgc ctgagtaaat
    36841 tcttctgccg ccatacgaat aatttgtacg ttatcaatat ggttagctgc gatgttgtat
    36901 tgcgcagcag caaccgacgg cttagcgatt tcggtggcta atacccgatc aaaattgcgc
    36961 gccagcgcta atgaaaagtt accgttgccg cagtacagct ccagtaaatc gccttttgag
    37021 cctttggtta cgtccagcgc ccattccagc atctgaatat tcatcgccgc gttcggctgg
    37081 gtaaagctgt tttctacctg acggtagatc atctctttcc ctgcgaccgg cagacgctcg
    37141 tcgatgtaat cttgatccag ctcgattttg gttttcgttg cccgaccaat cagatgcaca
    37201 ttcagattct gcgcgcgcag tgcatcgcgc agggcctccg cttcctgacg ccactcatca
    37261 tccagcttct tatggtatag cagggaaacc accgcctgat tactcagcgt ggtgaggtaa
    37321 tcaatctgga acaacttgtg gcgcagaacg ggattattac gcacacccgc aatcatcgcc
    37381 gtcatcaact ggttgataag ttcactggcg gcggggaagc tatccacgcg gatacggctt
    37441 ttggtttgtt gatcgaaaat gatgtgatac aggtcatcgc catcgtgcca gatgcggaac
    37501 tccgcacgca tccggtaatg actgaccggc gagcgaaaca cttccggaac caggtcagaa
    37561 aacggtgcca tcatactttg caaacgtacc actttttcgg ctaactgcgc ttcatactgt
    37621 tctgttggaa ggtgttcggg ggtcatgatg tatcctgaaa aattaaagta cgcggcgatt
    37681 gtagggattg ctcatcagat gtccagatct tgatgaattc ctatttgtga gctacgtctg
    37741 gacagtaact tgttacaacc tgtagcatcc acttgtcggt cctgtgagtt aatagggaat
    37801 ccagtgcgaa tctggagctg acgcgcagcg gtaaggaaag gtgcgatgat tgcgttatgc
    37861 ggacactgcc atccggtggg aagtcatcat ctcttagtat cttagatacc cctccaagcc
    37921 cgaagacctg ccggccaacg tcgcatctgg ttctcatcat cgcgtaatat tgatgaaacc
    37981 tgcggcatcc ttcttctatt gtggatgctt tacaatgatt aaaaaagctt cgctgctgac
    38041 ggcgtgttct gtcacagcct tttccgcttg ggcacaggat accagcccgg atactctcgt
    38101 cgttactgct aaccgttttg aacagccgcg cagcactgtg cttgcaccaa ccaccgttgt
    38161 gacccgtcag gatatcgacc gctggcagtc gacctcggtc aatgatgtgc tgcgccgtct
    38221 tccgggcgtc gatatcaccc aaaacggcgg ttcaggtcag ctctcatcta tttttattcg
    38281 cggtacaaat gccagtcatg tgttggtgtt aattgatggc gtacgcctga atctggcggg
    38341 ggtgagtggt tctgccgacc ttagccagtt ccctattgcg cttgtccagc gtgttgaata
    38401 tatccgtggg ccgcgctccg ctgtttatgg ttccgatgca ataggcgggg tggtgaatat
    38461 catcacgacg cgcgatgaac ccggaacgga aatttcagca gggtggggaa gcaatagtta
    38521 tcagaactat gatgtctcta cgcagcaaca actgggggat aagacacggg gaacgctgtt
    38581 gggcgattat gcccatactc atggttatga tgttgttgcc tatggtaata caggaacgca
    38641 agcgcagaca gataacgatg gttttttaag taaaacgctt tatggcgcgc tggagcataa
    38701 ctttactgat gcctggagcg gctttgtgcg cggctatggc tatgataacc gtaccaatta
    38761 tgacgcgtat tattctcccg gttcaccgtt gctcgatacc cgtaaactct atagccaaag
    38821 ttgggacgcc gggctgcgct ataacggcga actgattaaa tcacaactca ttaccagcta
    38881 tagccatagc aaagattaca actacgatcc ccattatggt cgttatgatt cgtcggcgac
    38941 gctcgatgag atgaagcaat acaccgtcca gtgggcaaac aatgtcatcg ttggtcacgg
    39001 tagtattggt gcgggtgtcg actggcagaa acagactacg acgccgggta caggttatgt
    39061 tgaggatgga tatgatcaac gtaataccgg catctatctg accgggctgc aacaagtcgg
    39121 cgattttacc tttgaaggcg cagcacgcag tgacgataac tcacagtttg gtcgtcatgg
    39181 aacctggcaa accagcgccg gttgggaatt catcgaaggt tatcgcttca ttgcttccta
    39241 cgggacatct tataaggcac caaatctggg gcaactgtat ggcttctacg gaaatccgaa
    39301 tctggacccg gagaaaagca aacagtggga aggcgcgttt gaaggcttaa ccgctggggt
    39361 gaactggcgt atttccggat atcgtaacga tgtcagtgac ttgatcgatt atgatgatca
    39421 caccctgaaa tattacaacg aagggaaagc gcggattaag ggcgtcgagg cgaccgccaa
    39481 ttttgatacc ggaccactga cgcatactgt gagttatgat tatgtcgatg cgcgcaatgc
    39541 gattaccgac acgccgttgt tacgccgtgc taaacagcag gtgaaatacc agctcgactg
    39601 gcagttgtat gacttcgact ggggtattac ttatcagtat ttaggcactc gctatgataa
    39661 ggattactca tcttatcctt atcaaaccgt taaaatgggc ggtgtgagct tgtgggatct
    39721 tgcggttgcg tatccggtca cctctcacct gacagttcgt ggtaaaatag ccaacctgtt
    39781 cgacaaagat tatgagacag tctatggcta ccaaactgca ggacgggaat acaccttgtc
    39841 tggcagctac accttctgaa ccacgtccca ccgtgctggt gtttgactcc ggcgtcggtg
    39901 ggttgtcggt ctatgacgag atccggcatc tcttaccgga tctccattac atttatgctt
    39961 tcgataacgt cgctttcccg tatggcgaaa aaagcgaagc gtttattgtt gagcgagtgg
    40021 tggcaattgt caccgcggtg caagaacgtt atccccttgc gctggctgtg gtcgcttgca
    40081 acactgccag taccgtttca cttcctgcat tacgcgaaaa gttcgacttc ccggttgttg
    40141 gtgtcgtgcc ggcgattaaa cctgctgcac gtctgacggc aaatggcatt gtcggattac
    40201 tggcaacccg cggaacagtt aaacgttctt atactcatga gctgatcgcg cgtttcgcta
    40261 atgaatgcca gatagaaatg ctgggctcgg cagagatggt tgagttggct gaagcgaagc
    40321 tacatggcga agatgtttct ctggatgcac taaaacgtat cctacgcccg tggttaagaa
    40381 tgaaagagcc gccagatacc gttgtattgg gttgcaccca tttccctcta ctacaagaag
    40441 aactgttaca agtgctgcca gagggaaccc ggctggtgga ttctggcgca gcgattgctc
    40501 gccgaacggc ctggttgtta gaacatgaag ccccggatgc aaaatctgcc gatgcgaata
    40561 ttgccttttg tatggcaatg acgccagaag ctgaacaatt attgcccgtt ttacagcgtt
    40621 acggcttcga aacgctcgaa aaactggcag ttttaggctg atttggttga atgttgcgcg
    40681 gtcagaaaat tattttaaat ttcctcttgt caggccggaa taactcccta taatgcgcca
    40741 ccactgacac ggaacaacgg caaacacgcc gccgggtcag cggggttctc ctgagaatct
    40801 caacagagaa aagcaaaaaa atgcttgact ctg
//
LOCUS       DAJVQG010000016        39842 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJVQG010000016 DAJVQG010000000
VERSION     DAJVQG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39842
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.016"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(23..98)
                     /locus_tag="OQM83_000805"
     tRNA            complement(23..98)
                     /locus_tag="OQM83_000805"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(63..65),aa:Lys,seq:ttt)
     gene            complement(147..222)
                     /locus_tag="OQM83_000806"
     tRNA            complement(147..222)
                     /locus_tag="OQM83_000806"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(187..189),aa:Lys,seq:ttt)
     gene            complement(226..301)
                     /locus_tag="OQM83_000807"
     tRNA            complement(226..301)
                     /locus_tag="OQM83_000807"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(266..268),aa:Val,seq:tac)
     gene            complement(451..526)
                     /locus_tag="OQM83_000808"
     tRNA            complement(451..526)
                     /locus_tag="OQM83_000808"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(491..493),aa:Lys,seq:ttt)
     gene            complement(691..1482)
                     /gene="cpoB"
                     /locus_tag="OQM83_000809"
     CDS             complement(691..1482)
                     /gene="cpoB"
                     /locus_tag="OQM83_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCS1519137.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1492..2013)
                     /gene="pal"
                     /locus_tag="OQM83_000810"
     CDS             complement(1492..2013)
                     /gene="pal"
                     /locus_tag="OQM83_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCS1519138.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(2048..3340)
                     /gene="tolB"
                     /locus_tag="OQM83_000811"
     CDS             complement(2048..3340)
                     /gene="tolB"
                     /locus_tag="OQM83_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCS1519139.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3473..4705)
                     /gene="tolA"
                     /locus_tag="OQM83_000812"
     CDS             complement(3473..4705)
                     /gene="tolA"
                     /locus_tag="OQM83_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCS1519140.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASSAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            complement(4770..5198)
                     /gene="tolR"
                     /locus_tag="OQM83_000813"
     CDS             complement(4770..5198)
                     /gene="tolR"
                     /locus_tag="OQM83_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCS1519141.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5202..5894)
                     /gene="tolQ"
                     /locus_tag="OQM83_000814"
     CDS             complement(5202..5894)
                     /gene="tolQ"
                     /locus_tag="OQM83_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCS1519142.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5891..6295)
                     /gene="ybgC"
                     /locus_tag="OQM83_000815"
     CDS             complement(5891..6295)
                     /gene="ybgC"
                     /locus_tag="OQM83_000815"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCS1519143.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPCALPKSIVAEFKQ"
     gene            complement(6445..6738)
                     /gene="ybgE"
                     /locus_tag="OQM83_000816"
     CDS             complement(6445..6738)
                     /gene="ybgE"
                     /locus_tag="OQM83_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCS1519144.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6738..6851)
                     /gene="cydX"
                     /locus_tag="OQM83_000817"
     CDS             complement(6738..6851)
                     /gene="cydX"
                     /locus_tag="OQM83_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCS1519145.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6866..8005)
                     /gene="cydB"
                     /locus_tag="OQM83_000818"
     CDS             complement(6866..8005)
                     /gene="cydB"
                     /locus_tag="OQM83_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCS1519146.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(8021..9589)
                     /gene="cydA"
                     /locus_tag="OQM83_000819"
     CDS             complement(8021..9589)
                     /gene="cydA"
                     /locus_tag="OQM83_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCS1519147.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(10437..13070)
                     /gene="mngB"
                     /locus_tag="OQM83_000820"
     CDS             complement(10437..13070)
                     /gene="mngB"
                     /locus_tag="OQM83_000820"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCS1519148.1"
                     /translation="MKAVSRVHITPHMHWEREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLQDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGGVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(13088..15004)
                     /gene="mngA"
                     /locus_tag="OQM83_000821"
     CDS             complement(13088..15004)
                     /gene="mngA"
                     /locus_tag="OQM83_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCS1519149.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTTYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            15173..15895
                     /gene="mngR"
                     /locus_tag="OQM83_000822"
     CDS             15173..15895
                     /gene="mngR"
                     /locus_tag="OQM83_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCS1519150.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15999..16868)
                     /gene="sucD"
                     /locus_tag="OQM83_000823"
     CDS             complement(15999..16868)
                     /gene="sucD"
                     /locus_tag="OQM83_000823"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCS1519151.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16868..18034)
                     /gene="sucC"
                     /locus_tag="OQM83_000824"
     CDS             complement(16868..18034)
                     /gene="sucC"
                     /locus_tag="OQM83_000824"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCS1519152.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(18128..19345)
                     /gene="odhB"
                     /locus_tag="OQM83_000825"
     CDS             complement(18128..19345)
                     /gene="odhB"
                     /locus_tag="OQM83_000825"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCS1519153.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(19360..22161)
                     /gene="sucA"
                     /locus_tag="OQM83_000826"
     CDS             complement(19360..22161)
                     /gene="sucA"
                     /locus_tag="OQM83_000826"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCS1519154.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22462..23178)
                     /gene="sdhB"
                     /locus_tag="OQM83_000827"
     CDS             complement(22462..23178)
                     /gene="sdhB"
                     /locus_tag="OQM83_000827"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCS1519155.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYPLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(23194..24960)
                     /gene="sdhA"
                     /locus_tag="OQM83_000828"
     CDS             complement(23194..24960)
                     /gene="sdhA"
                     /locus_tag="OQM83_000828"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCS1519156.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLHRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24960..25307)
                     /gene="sdhD"
                     /locus_tag="OQM83_000829"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24960..25307)
                     /gene="sdhD"
                     /locus_tag="OQM83_000829"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCS1519157.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(25301..25705)
                     /gene="sdhC"
                     /locus_tag="OQM83_000830"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(25301..25705)
                     /gene="sdhC"
                     /locus_tag="OQM83_000830"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCS1519158.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            26086..26193
                     /locus_tag="OQM83_000831"
     CDS             26086..26193
                     /locus_tag="OQM83_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1519159.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            26399..27682
                     /gene="gltA"
                     /locus_tag="OQM83_000832"
     CDS             26399..27682
                     /gene="gltA"
                     /locus_tag="OQM83_000832"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCS1519160.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            28072..28638
                     /locus_tag="OQM83_000833"
     CDS             28072..28638
                     /locus_tag="OQM83_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1519161.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28689..31147
                     /locus_tag="OQM83_000834"
                     /pseudo
     CDS             28689..31147
                     /locus_tag="OQM83_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            31162..31893
                     /locus_tag="OQM83_000835"
     CDS             31162..31893
                     /locus_tag="OQM83_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS1519162.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31890..32951
                     /locus_tag="OQM83_000836"
     CDS             31890..32951
                     /locus_tag="OQM83_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1519163.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            33103..34149
                     /locus_tag="OQM83_000837"
     CDS             33103..34149
                     /locus_tag="OQM83_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCS1519164.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(34146..34937)
                     /gene="nei"
                     /locus_tag="OQM83_000838"
     CDS             complement(34146..34937)
                     /gene="nei"
                     /locus_tag="OQM83_000838"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCS1519165.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGLVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34973..35707)
                     /gene="pxpA"
                     /locus_tag="OQM83_000839"
     CDS             complement(34973..35707)
                     /gene="pxpA"
                     /locus_tag="OQM83_000839"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCS1519166.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(35697..36629)
                     /gene="pxpC"
                     /locus_tag="OQM83_000840"
     CDS             complement(35697..36629)
                     /gene="pxpC"
                     /locus_tag="OQM83_000840"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCS1519167.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(36623..37279)
                     /gene="pxpB"
                     /locus_tag="OQM83_000841"
     CDS             complement(36623..37279)
                     /gene="pxpB"
                     /locus_tag="OQM83_000841"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCS1519168.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(37302..38045)
                     /gene="ybgI"
                     /locus_tag="OQM83_000842"
     CDS             complement(37302..38045)
                     /gene="ybgI"
                     /locus_tag="OQM83_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCS1519169.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            38316..39797
                     /gene="dtpD"
                     /locus_tag="OQM83_000843"
     CDS             38316..39797
                     /gene="dtpD"
                     /locus_tag="OQM83_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCS1519170.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         9649 a        10714 c         9701 g         9778 t
ORIGIN      
        1 atgctgcttt aatcattcag gatggtgggt cgtgcaggat tcgaacctgc gaccaattga
       61 ttaaaagtca actgctctac caactgagct aacgacccat acgggtgtta cctgaaagat
      121 ttctactcgg ggccaatctt taagattggt gggtcgtgca ggattcgaac ctgcgaccaa
      181 ttgattaaaa gtcaactgct ctaccaactg agctaacgac ccgagtggtg ggtgatgacg
      241 ggatcgaacc gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc
      301 cgatactgcg ctggatacta caaactactt ccaccgaacc caccgtaatt agtggtgggt
      361 cgtgcaggat gactcgcttc gctcgcccta cgggccgttg ctgacgcaac gttatcctac
      421 acgcttaaca tctgtcttcg atgctaaaac tggtgggtcg tgcaggattc gaacctgcga
      481 ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccactt tttcgttgct
      541 ttcggtttgt ttgatatccc gtggcaacgg cggcatatat tactgatttc agacttgagc
      601 gcaacaaaaa tttcgatgca gatcactcaa ctgcttatga ttcgcacgac acgaccagaa
      661 ataatgcgac ttctggtcgt gtgttatgca ttacatcgcg ttcagacgtt tttgtgcctg
      721 tttagcgcca tcagtaccag ggtatttact gataacctgc tggtatacgg ctttcgcttt
      781 tgcggtgtca cctttgtcct gcatgatgac gccgacttta aacatcgcat cagcagcctt
      841 tggtgacttc ggatagtttt tcactaccga agcaaaatag tacgccgcat catctttttt
      901 acccttgttg tagtttaact gacccagcca ataattggcg tttggcaggt aagttgaatc
      961 agggtaattt ttgatgaaat tctgaaatgc caccattgcg tcatcctggc gggatttatc
     1021 ctgcaccagc gcaatagctg cattgtaatc cgtgtttgca tcgccgcttt tcaccggcgc
     1081 gccagcattc gcagtaccag catcagctgt cggcgtcgtt gatgccgccg caccgctttg
     1141 atcgctgctg gttgattgcg ccgctgcacc accgctgctg aggctgtcga tctgcaacag
     1201 gatctgcttt tgccgctcca cgacctgatt cagttgatac tgattttcct gaatctgacc
     1261 acgcagggaa tcaatatcgg attgattatc agaaagttgt tgctggagtt gggttaaaag
     1321 ctggctgtga gcattagaaa tacgctcaag ttgagtgacg cggtcttcga ccgagcctga
     1381 gccgacacta ctgattggtg cctgagcaaa agcggcccag ggggccgcta taccaaccag
     1441 taacgacaga ctcaatagtt gatgtctgaa gttactgctc atgcaattct cttagtaaac
     1501 cagtaccgca cgacggtttt tggagtatgc cgcttcgtca tgacccagta ctgcaggttt
     1561 ttctttaccg taagaaacga tggagatctg gtctgcagaa acgcctttac cctgcaggta
     1621 catcttaacg gcgttcgcac gacgttcacc cagggagatg ttgtattccg gagtaccacg
     1681 ttcgtccgcg tgaccttcta cggtgacttt gtaagacggg ttgctacgca ggaagtttgc
     1741 atgtgcatcc agcatttgag cgaagtcaga acggatatcg tacttgtcca gatcgaagta
     1801 aacgatgttg ttctgctgca gctgttgcat ttgcagacga gcctgctctt cggaagacat
     1861 gttgccgttg ccgccgttcg catccatacc agtgccggca cccagcatgc cttcgctgcc
     1921 gtcattgctg gcgttcttgt tggaagaaca tgccgcaatt gccataacag gcagagcaat
     1981 catcagccct ttcagcactt tgttcagttg catttcaatg attcctttac tattcaatta
     2041 attattatca cagatacggc gaccaggcag ggaatttgac ctgtccatca gttgccggaa
     2101 gacgcgcttt gaaacgccca tctgtagaaa ccaaattcag cacggatccc atcccctgag
     2161 aagagctgta gattaccata gtgccgttag gtgccagact tggcgtttca tccaggaacg
     2221 tggacgacag aacttgtacg cctcccgttg ccagatcttg tttggcaatg tgctgctgcc
     2281 caccgttgga gctgaccatt accataaatt taccgtcgct gctgacatcc gcatcctggt
     2341 tctgcgaacc ttcccaggta atacgttgtg gcgcaccgcc gttgatattc actttataaa
     2401 cctgtggacg accggcctgg tcagaagtaa atgccaggtt ctggctgtcc gggaaccagg
     2461 tcggttcggt attgttactg cgaccatcag tcacctggcg gatctgacca gaagccaaat
     2521 ccattacgta caggttcagg ctaccggttt tcgacaaggc gaatgccagt ttgctgccgt
     2581 ctggcgagaa tgcaggcgca ccgttgtgac gcgggaatga agccacctga cgtacagcgc
     2641 cgtttgccag cgtttggata actagcgcgg aacgaccgct ttcgaaggtc acataagcca
     2701 gtttagaacc gtctggtgac cacgccggag acatcagcgg ctgtggtgaa cggtgaacga
     2761 caaactggtt gtaaccgtca tagtcagata cgcgcagttc atacgggaac tgaccgccgt
     2821 tggtctgaac aacgtaggca atacgggtac ggaacgcacc tttaatgccg gtcagttttt
     2881 caaacacttc atcactggcg gtatgaccag catagcgcag ccactgcttg ttcactttgt
     2941 acgagttctg agcaagtaca gtacccggtg cgccgccagt gtcaacaagt tgataagcaa
     3001 cattgtaaga accatccgga ttcggagtga cctgaccgac aactacagcg tcaataccca
     3061 gcgcggacca tgcagctggt tgtacttcct gcgcactacc cggctgctgt ggcagacgag
     3121 cgcgatctaa cggattaaat ttaccgctgt tacgcaagtc ggcagcaacg atgccgccaa
     3181 tatcttcagg tgccgcacca gggcccgccc actggaaagg aacaacacca ataggacgac
     3241 cggaatctac accgctgtcg atcacaatgc ggacttcagc atgcagaact gatgcccaca
     3301 gtatgagaaa accaaatgct actcgtaatg cctgcttcat catatctccc ttatctggac
     3361 cgatggccca cgataattta gcagaatgtt aacaaactaa aatacacaaa actaccagaa
     3421 ccccgtggca accggtgcct gatgttgacc gtcggaacag tcaacatcgc gattacggtt
     3481 tgaagtccaa tggcgcgttt ttgaacactt catatactgc ctggcttggt ggtttcggga
     3541 tcttcgcaag tttagctgct gccaacgcag cctgacaaag tgcgggatcg ccaccttcag
     3601 gtttgatatc cagtaacata ccatcgggtg ccagttttat gcgcagcgta caggttttgc
     3661 ctgcatagga cgatgcgtca tagaacttac tttcgatagc agatttaatc tgcccggcat
     3721 agttattgat atcggcccct gatgcgccat tgtttttagt attaccactc ccggcagacg
     3781 aagcattgtt ccctttcgcc cctccccccg ttttcggtgc attcttacca gagcttagct
     3841 caccgaaaat atcatctgcc tctgcggcag cttttgctgc agcggctttt tcagctgctg
     3901 cttttgccgc tgccgctttt ttgtctgcgg cggctttttc tgccgctgct tttgccgctg
     3961 ccgctttttt gtctgcggcg gctttttctg ccgctgcttt cttatcagct gctgccttat
     4021 cagcagccgc tttttctgct gcttttttgt cggctgcagc tttctctgct gccgctttct
     4081 ttcttgcttc agctgcagca gcttctgccg cttccgcttt cttcttcagt gccgcagcgg
     4141 ctgcctcggc ttttttctgc gcttcggctg cggctttggc ggcttctgct tctgcttttt
     4201 tcttcgcgtc tgccgccgct ttcttcgctg cttcttccgc agctttagca tccgcttcgg
     4261 ccttcgcttt agcatctgcc gccgctttcg ctgccgcctc ttccgcttgc ttctgcttta
     4321 actcggcctg ttttgcggct tcttcagcct gctttttctg ttcctgagca gctaaccgct
     4381 ctttctcaag ttgcttcagg cgttcctgtt cagccgcttg tttctcacgc agttcttcag
     4441 cagcctgctg ttcctttatc ttgcgctgct catcagaacg cttcgcgctt gattcctggc
     4501 tttgcatgcg tttgtactgc tcaactaccg cacctgaatc aaccatgaca gcgtcgatgg
     4561 acgaaccacc gccgcctccg gctgaagctt ctatattctc atcgaacgaa ctccagagca
     4621 gcgccgcaaa taagatgaca tgcagcactg ctgaaataat tatcgcccgc ttgagcttgt
     4681 cgttttgttc ggttgccttt gacactctcg gtttccaaaa actgttcgcc tgttacccgc
     4741 tctctttcaa gcaagggaaa cgcagatgtt tagataggct gcgtcattaa accaaccgat
     4801 ttcacacccg cactatgtaa caagttcagt gctttaatta tttcatcgta aggcacatct
     4861 tttgcgccac cgatcagaaa gaccgttttc gggttggcct tgaaacggct ggacacttcc
     4921 gccaccacct gctctggtgg taaacgctcc aggcgatctt tctcaaccac cacggtgtac
     4981 tgaccaatac cagacacttc aacaatcact ggcggattat cgttactgct caccgcctgt
     5041 gattcagtag cgtctggcag atcgacctcc acgctctggg tgatgatggg cgctgtcgcc
     5101 ataaagatca gcaacagcac cagcagtacg tccagcaacg gtacaatgtt gatttcggac
     5161 ttgagatcgc gacgacctcg tccacgcgct ctggccatgg cttacccctt gttgctctcg
     5221 ctaacggtaa acgcctggcg gtgcagaatc gcggtaaact cttccataaa gttgtcgtaa
     5281 ttcagttcca gtttgtttac gcgctggttg aggcggttat aagccattac cgccggaatt
     5341 gcggcgaaca gaccaattgc agtcgcaatc aacgcttctg cgataccggg cgcaaccatt
     5401 tgcagtgttg cttgttttac cgccccgagg gcgataaagg cgtgcatgat cccccagacc
     5461 gtaccaaaca gaccaatata cgggctgatg gagccaaccg taccgaggaa cggaatgtgc
     5521 gtttccagat tttcaagttc acggttcatg gagatacgca tagcacgcga cgccccttcc
     5581 actacggctt ccggcgcatg gctattggca cgatgcaggc gcacaaactc tttgaacccg
     5641 ctgtaaaata tttgttccga accagtcaga ttatcccgtt tcccctgact ctcttgatag
     5701 aggcgagaga gttcgattcc agaccagaat ttatcttcaa acgcttcggc ttcgcgcgcc
     5761 gctgcgttaa gaatacgggt ccgctggata ataatggccc aggatgcgat tgaaaaacca
     5821 atcaaaatca acatgataag tttaaccaga aggctagcct tcaggaacaa atcaaggata
     5881 ttcatgtcag tcactgctta aactccgcga caatagactt gggaagcgca caaggcttca
     5941 ttttgagtgg gtcaacgcaa acaaccagaa cctctgcttc attcagcaaa gtattctcgg
     6001 cgttgacaat acgttgcgtg aaaaccaaag aggtgccacg cattgatgtt atttcagtct
     6061 gtatttcgag catatcgtcg agccgcgcag gtgcgtaata ttccaccgtc attttacgta
     6121 ccacaaaggc aacgcgttca gccatcagcg cctgctgact gaagtgatga tgacgcagca
     6181 tctctgtgcg tgctctttca taaaaagcga cgtaactggc gtggtacacc acaccaccgg
     6241 catcggtatc ttcatagtag acgcgaaccg gccatcgaaa cagcgttgta ttcactttac
     6301 atcccggtaa tgcaacaaaa gttagagctt ttaaacgctg ctactatacg cgcgcgattg
     6361 aggtttggga atgagggcaa gttaagggag cgtaaaaatt tatgggcctg gagcggccca
     6421 taatttttta aagaaaattc tgatttaaaa gaagaagaaa atcagcccga caatgagaac
     6481 aatatcggca agcaatgggc aaaaaatccc ttgccaaaga accttctgcg gacgaaagcc
     6541 cacgccgtga atcacaccag cacagacggc ccacatcagc aataaaccat gccagatttc
     6601 cagttcactg gtcttcgcgg caaaacgaga tgggtcccaa aacatacatc ctgctaacag
     6661 aagcgccatc acgaaggaaa gcgcccgcag ggggcgcttg tccattaccg catacaaggt
     6721 cgcgataatc ttactcatca gatgtcttct tgaccggctt tgcctgattc gacgtgttca
     6781 agcgccagcg cggtgattac cccaaacgaa caggcaagaa gcgttcccag aatccatgcg
     6841 aaataccaca tttttagctc cttacttagt acagagagtg ggtgttacgt tcaatatctt
     6901 ctttggtgat acgaccgaac attttccagt aacaccaagc ggtgtagagc agaatgatcg
     6961 gtaccagaac caccgcaacc caggtcatga cgttaagcgt cagctggctg gaagttgcat
     7021 cccacattgt cagacttgcg ttcatcatgg tgctggacgg catcacaaac gggaacattg
     7081 cgataccggc tgtcaggatg atgcaggcca gcgtcaggga ggagaacaca aacgcccacg
     7141 cggctttatc catacgtgca gtcaggatgg tcagcagcgg aagaaccaca cccagtgccg
     7201 gaatagccca cagaattggc gtgttgttga agttaaccag ccatgcgcca gcttcacgaa
     7261 ccacttcttt attcagtggg ttagaggctg cgtaatggtc cattgtcgat ttcacgacat
     7321 aaccatcgat accgtacatc acccatacgc cagccagtgc gaaacagacc agtgtcacca
     7381 gcgcagccac ctgagccgtt gcacgggtac gcaggtgcag ttcgcccacg gtacgcattt
     7441 gcagataggt tgcgccctga gtaatgatca tccctacgct caccacgcct gccagcaggc
     7501 cgaacgggtt aagcaactgg aagaagttac cggtgtagta cagacgcaga tattcatcaa
     7561 cgttgaacgg tacgccctgc aacaggttac cgaacgctac accaattacc agcggcggaa
     7621 cgaaactacc aatgaagatg ccccagtccc acatgttacg ccagcgggtt tcttcaatct
     7681 tggagcggta gtcaaaaccg accggacgga agaacaaaga cgccagcacg aggatcatcg
     7741 ccacatagaa gccggagaac gcagcggcat agaccatcgg ccaggcagca aagagtgcgc
     7801 cgcccgcggt gatcagccaa acctggttac cgtcccagtg tggtgcaatg gagttaatca
     7861 taattcgacg ctcggtgtcg ttacgaccga ggaaacgggt gagcatgccc acccccatgt
     7921 cgaaaccgtc agtgactgca aaaccaatca gcagaacgcc aaccagcagc caccagataa
     7981 aacgcaatac ttcataatcg atcatttgac gactcctgtc ttagcgtgcc ggctgagtag
     8041 tcgtggaaga ctgctcaaag tgatagcgac cggttttcag gctgcttggg ccgaggcgtg
     8101 caaacttgaa cattaagaac aattctgcca ccaggaacag ggtatacagg ccgcaaatca
     8161 gcaccattga gaagatgaga tcgcctgcgg tcagtgacga gttcgccaca gctgtcggca
     8221 gcacttcacc gatagcccac ggttggcggc catattcagc cacgaaccag cccgcttcta
     8281 cagcaatcca cggcagcgga ataccgtaca gcgcggcgcg cagaagccat tttttctcgc
     8341 caatgcggtt gcggatgaca ctccagaaag agagcgcgat gattgccaga agcaggaagc
     8401 cacacgccac catgatacgg aacgcaaagt acagcggcgc tacacgcggg atggagtctt
     8461 tggttgcctg ttgaatctgc gcttcagtcg catcagccac gtttggcgta tagcgtttca
     8521 gcagcagacc gtaaccgagg tctttcttca tgctattgaa ctggtcacga accgcctggt
     8581 cggtagaacc agaacgcagt tgttcgagca gagagtacgc cttcatcccg ttacgaatgc
     8641 gttcttcatg ctgcaccatc agctctttca ggccgataac cggggtatcc acggaacgcg
     8701 ttgcaatgat gcccagtgcg taagggatct gaatcgcaaa tttgttcgtc tcctcttcct
     8761 gatcaggaat gccgaacaga gtaaaggcag caggcgcagg ttgcgtttcc cactcggctt
     8821 caatagcagc cagtttggtt ttctgcacgt cgcccatttc gtagccggat tcatcaccca
     8881 gaacaataac agacagaaca gcagccatac cgaagctggc agcgatagca aaggagcgtt
     8941 tagcgaaggc gaagtcacga cctttcagca tataccatgc gctgataccg aggatgaaca
     9001 tcgcgccagt cacataacca gacgctacag tgtgaacgaa tttcacctga gcaaccgggt
     9061 taagcaccag ctcggagaag ctcaccatct ccatacgcat agtttcaaag ttgaaatcgg
     9121 acgcgattgg gttttgcatc cagccgttcg caaccagaat ccacagtgcg gacaggtttg
     9181 aaccgagcgc caccagccag gtgacacaca tatgctgaac tttacccaga cgatcccaac
     9241 cgaagaagaa cagacctaca aaggtggatt cgaggaagaa ggccatcaga ccttcgattg
     9301 ccagcggcgc accgaagata tcccctacat agtgggaata gtaagaccag ttagtcccga
     9361 actggaactc catggtcaga ccggtagcca cacccagagc gaagttgata ccaaacaact
     9421 tgccccagaa cttggtcata tctttataaa tctgtttgcc ggagaggacg tagaccgttt
     9481 ccataatggc cagcaggaac gccataccga gcgtcagtgg cacaaaaagg aagtggtaca
     9541 tcgcggtcaa ggcaaactgt aagcgcgaca gttcgactat atctaacatc atgactcctt
     9601 gctcatcgca tgaagactcc gagagtgaac cccgttagaa agggtcacac gcatgcccca
     9661 atagaaattt gtttgaacac tttcatcata ccatgtgaat gcacacaaaa acggcgtaaa
     9721 gaaaggttgc aaacatgtta ataaaaactc aaattgatcc cacgtatata ttacgctgca
     9781 aaatccttac aataaacagg tttttgttga agcaaatttg catttttcga cggtgatcaa
     9841 tttatagcta aattaccgcc tttcagccaa tttgatcgag aacaatttat ctctttttga
     9901 tgccaatttc caagacttat acattgataa atatcaattc ctacaaatta tgttaatcat
     9961 gtttcaataa ggtaacttta gtgacattta tgtttaaaat gtgtgagtta taaggctgaa
    10021 attagtttcg gttggcaatt taaagataaa aagagacgaa gcaaaaacag cacggttgac
    10081 cttaattctt tttttggtca ctggctaata cgacgcagag tttcaggatt actaataaag
    10141 gcataaatcc accccgccga tattaacatt taattaactt gtaattatat tataattaac
    10201 tcacccaggg ttattatttt gttaaaaata aagccacata tacgtggctt gagagtgaag
    10261 ataaacgcaa aattacctga tgcgctacgc ttgccagtgt ctacatcatc tctgcaatat
    10321 attgaatttg cctgcttttg tagaccgaat aaagcgttca tgccgtatcc ggcatgaaca
    10381 aagcgccctt tgtcaacaat ctggccgcgc atatgtggct tcggtttgat tgctattcag
    10441 gcaagccggt aactgaacgt ccgtgactgg ccgggtaaaa aaggccccga tagatttgaa
    10501 ccttgatttt cctcggtggt aatgtgttca tccatcatcg tttctgagca agaaatcacc
    10561 tcgcgactga aagcaacagt cgcatcacag gttgctgatt cagccggatt aaacagccgt
    10621 aaaatcactt cttgtcggtc ttcagctttc ttaagtgcgc ttatcaggca tcccactggg
    10681 ggcattttca acaaactata actttccggc acgttgaatc cggctttgtt gagcttcatc
    10741 gcatcccatg ggattttgtt gtaacactgt actggagtca gccatgctcg cgcctgctga
    10801 gctacaccag cggcggttgg cgtaccggta taactcaata aacttaggcg acaagaaagc
    10861 agaccacgta gttgtgagtc cgggactggc attttaattc ccgaaggccg cccaggcctt
    10921 aaaagcagat cttctttgcc cagtaagccc acgccacgca gcaacgtaat ggcaaaggtt
    10981 ttcttctctt caccgatgac ttcaaattca cgtaaccctt cgctaaagac agccatgccg
    11041 ttacgccctt cctgtaaggc aacatagttg agcatattcc ataccggaac cggcgcttct
    11101 ttccagcctt cttgctgcca gttgttcatt gcactgtcgt tcacggggcg cgttagcgaa
    11161 ccaaactgcg tatctgccag aacactgtca gtgttaaaag gtgtagggac caggacacga
    11221 aggcgatgat cgtcagcctg gttatcaagg ttgatatcca catcaatacg cctgctgtta
    11281 tgactaagag tgacaaccaa caccccccct actctgccag tggattgccg ggcgctgcgt
    11341 tctgacaaat tgagcggcac tgccatgtca tagcggataa cagccctgct ctgccaggct
    11401 tcatgaataa tatcgcattg cggtttcgcg ttcgctgcgg taattaccca ctcttctttt
    11461 gcgggtgaat agtcatattc atcaccatca tcagagcttt cttcaatttg caatacccgg
    11521 tcatagcgca caccgctgtc tttatctacc agttgcagag aaccatcctc attgagcgca
    11581 atctgccaga aagcattttc cagaatccct tcagcgtcac tttttgccgc aatcacgttg
    11641 ccaggctgat tcgcttcaat ataaagcgtg cgatagccca tagaagggac aatctggttg
    11701 atctgaatat caaactccat aaagggatca taattgccat aatgaacaat ttgccgatcg
    11761 attaggccag gatcgatctc acgggcatgg cgaataaaat acggtacagg ctgaccgcga
    11821 tcgtcttgta gattaaactg gctggcgcgc agccgcacag tggtgttaat aacttcttcc
    11881 cgcggccagg gcatcaggtt aaacagcacg agtttgtcgg catcactctg tggcatgtta
    11941 tcggcaattt tgcgcatgta gaaacgcagc agattatccg ccatgtcttc agccagttcg
    12001 aagcgggcga caatttcgcg atgaactttg tcactgcaac aacaaccgat actgtcgtgg
    12061 gcatgatttt ttaagatctc tttccacatt ttctccagca agccgtggtg gtattcaaaa
    12121 cccaacgtcc aggccagcgt tgccagcggt tccagcagat taacaatctt attttcaata
    12181 cgtgcgtggg caattttgat atccatgcgc gtggaaccga tggtgcgatg tacgcgcata
    12241 tatttgccat cgataaattc acctttcagg gtggccagac tttcacgctg cgcttcgatc
    12301 ttctcaaata cctcttcaaa gcggctcatc acaaacttac gttgagggta gatctcacgt
    12361 agcttatcca tcacttcgaa gatattttgc tgcaatggca tctggtcatg accattcggc
    12421 agcaaaatct ctttggttac agacgctttt tccagcacgt caaaataact gtcgaggcgt
    12481 ttacgtaatc cgttttcgtc ggcaggtaag tacttaccga tggcgtagcc cagcggcagc
    12541 acctgcgccg tcacttcgct accgtcactg ctttgccaca aaaactcggt tttatcagta
    12601 ccgtggcgct ccgaacatcc gcgccagaac atagtgcggg taatgccaaa tccattgtaa
    12661 atatgcggca gttgcccgga catgccaaag gaatccggta aataacctat tttcatcggc
    12721 tcgccaaacg cgaggcagtc acgcattccg tacatcagat tacggacgat ggattccgca
    12781 gaaacaatcg tggtatcggt ctgggtatac caggggccga taatcaactt accggcttct
    12841 acctgtttct tcacgcggtc tttgttttcc ggtttcaccg cgaaataatc ttcgaggatc
    12901 gccgtttgcc cgtcgagtac gtaatatttg tattcgttgt cctgttccag tcggcacagg
    12961 atctcttcca tattattgac cagcagaatg cgtgactctt cggtggtgaa ataccactcc
    13021 cgctcccaat gcatatgcgg ggtgatgtga acgcgagata ccgctttcat cgtcgtttcc
    13081 tttttgttta tggcattacg ccatcagtca gatagttgcc atgcttaacc gcgtgacgcc
    13141 gccagatcag gagaattgca gtcgagattg cagcccccac caatgccgca ccaaaccagc
    13201 caattgccgc cataacaccg cccgcgccat tatcatgaag taaaaagagc gagaaaatgc
    13261 cggcaccggg tgtcgaaagg ccgatattca tcgcaccgac aatagcgccc gttaccatag
    13321 agcccagcac aaacgaacca ataacccgca gcggatcttc aatcgccatc gggatcgccc
    13381 cttcggtaat acctgccagc cctaacagcc aggtggattt cccggtctca atttcaaact
    13441 ctttaaacag gcgcggtgcg agcatcgtgg aagcggttac ggtaaatgcc gaaaccattt
    13501 tgacggaggc gaaaatggca tacgggccgt aaacgccgtt cgccattgcg cccaggcaga
    13561 atgcataagc ggctttgttc actggccctc caaggtcaaa ggaacacata aaaccgagaa
    13621 tggcacccag caacagcgcg ttacttcctg acagaccgtt cagccaggcg gtaagcgagt
    13681 tattgatcca tgcgacaggt tctcccacca caaacagcat cagactgccc gctcccaacg
    13741 taccgagcac cgggtagaga taaaaagtca ggaatccatt gaatttactg ctaagacgca
    13801 agtgattttt cacccagcgc atcaagtaac cggctatcaa tccgccaacg accgcgccga
    13861 gaaacccgga gccgatcatg ttggcggcaa gtccagccgc aaagcctggc gctaacgccg
    13921 gtttatctgc cagagaatag gtggtatagg ccgcgagcac cggtaccatc aaaattccga
    13981 gcagcccgcc gcccagcttg cggtacatcc acagccagga gttttcttca ttaaacagat
    14041 cttgtagccc gaagatttgc gacagtaata ccgcgaccgc cagcaccgtg ccccccgcga
    14101 caatcaacgg tacggcaaaa gagattccgc tcaacagtgc ctgtttcagc tccgttttga
    14161 cacttttcac cggttgcgta tcttgctgta cggtacgcgt ctcatcgcta cgcttgaggg
    14221 taagcgcttg ttggatcaac gcttctgcat ggcgaatcgg ctcggcaaca ggcactgaaa
    14281 gcgcgggaat gccattaaaa cgctcactct ccttgatggc gacttcagcc gcaaaaatac
    14341 aggcggttgc actattgagt tgatccgccg ttaaacgccc ttcaatgccg ttagcgcctt
    14401 gtttttcaac gtaaacattt acgccgagtt tgcgtccggc tttttccaga tattccgcag
    14461 ccatataggt gtgagcaata cccgccggac aggccgttac gcagacgata gttggcgcgt
    14521 tggaaaaaga ggcagaaggt tgcgtgcctc ccttgtcatc cagcgccgag agcaactcat
    14581 caggcgtcgt cgccgactgt atacgcgccc gaatctcatc atccgcaagg cgcgtggtca
    14641 gcgctgtcag cagttgcata tgcgtagtac ccgcttcatt ggggggaatc gccagcagca
    14701 ccactaaatc aactgcttcc gggccatcaa cgccttccca ctgaagcggc tcgctgagtg
    14761 tggcgaccgc aaacgccgct tcttttaccg cagcagtttt gccatgcggc acagccaacc
    14821 cttcacctaa ggcggtcggg ccaaggcttt cacggcgata cacttcttcc agaaattgct
    14881 cagtactgga aattttcccc agagcagcaa gacgttgagt caacgcgtgg atggcctctt
    14941 cacggctggt aaagcgcgca ttcagacaca acgcatcgcg gtgggtcaga gtcgtcagat
    15001 tcatcatgat cctcacttga tcgtttttat agtcgcgcca gtgtgcccga taaaacaata
    15061 ccatcatgtg atcaatttca cttgattgaa attatttgta ttgtatttgt attatttaca
    15121 ggatgaattt atcgggcata atggctaaat tcatattaat gagatactaa aaatgggaca
    15181 caagccctta taccggcaga ttgccgatcg cattcgtgaa caaattgcgc gtggcgagct
    15241 taaacctggc gatgcgttac ccactgaatc agcgctacaa acagagtttg gcgtcagccg
    15301 ggttacggtg cgtcaggctt tgcgccagct agtcgagcag cagatcctcg aaagcattca
    15361 gggcagcggg acttacgtca aagaagagcg ggtcaattac gatatttttc agttaaccag
    15421 ttttgatgaa aaactgtcgg accgtcacgt cgatacacac agtgaaatcc tgatatttga
    15481 agtgattccg gctgacgatt ttcttcagca acagctacaa atcaccgcgc aggatcgtgt
    15541 gtggcatgtg aagcgcgtgc gctatcgcaa gcaaaagcca atggcgctgg aagaaacctg
    15601 gatgccgctt gcgttgttcc cggatctcac ctggcaggtc atggaaaatt cgaaatatca
    15661 ctttattgaa gaagtgaaga agatggttat cgatcgtagc gaacaggaaa tcattcctct
    15721 gatgccaacc gaagagatga gccgtctgct taatatcagc cagacaaagc cgattcttga
    15781 aaaagtatcg cgtggatatt tagttgatgg tcgggtgttt gaatatagcc gcaacgcttt
    15841 taataccgat gactataaat ttaccctgat agcgcagcga aaatcatcgc gataagcaca
    15901 aaaaaggcca tcatacgatg gccaaccatg tcgaaaccga cagcccttcg ccattcctgt
    15961 agagggaagc ggcgagggct atttcttatt acagatattt atttcagaac agttttcagt
    16021 gcttcaccga tatccgccag gctgcgaacg gttttcacgc ctgcggcttc cagagcagcg
    16081 aatttctcat ccgcagtccc tttcccacca gcaatgatcg cacccgcgtg gcccatacgt
    16141 ttgcctttcg gcgcagtcac accagcgatg taacctacaa ctggcttggt aacgtgctct
    16201 ttgatgtacg ctgccgcttc ttcttcagcg ctaccgccga tctcaccgat catcacgatc
    16261 gcttcggtct gcggatcttt ttcgaacatt tcgaggatgt cgataaagtt agagcctggg
    16321 atcgggtcac cgccgatacc aacacaggtc gactgaccga aaccgtaatc cgtggtctgt
    16381 ttaaccgctt cataggtcag tgtaccggaa cgggaaacga tacccacttt acccggtttg
    16441 tgaatgtgac ccggctggat accgattttg cattcacccg gagtgataac gcccgggcag
    16501 ttcgggccga tcatacgaac gccggcttcg tccagtttca ctttcacagt cagcatatcc
    16561 agcgtcggga tgccttcagt gatggtgata atcagtttga tgcctgcatc aatagcttcc
    16621 agaatggagt ctttgcagaa cggtgctggt acgtagataa cagaagcggt agcgccagta
    16681 gcagcaacag cttcacgcac ggtgttgaac accggcaggc cgaggtgggt agtaccgcct
    16741 ttacctgggg ttacgccgcc aaccatttta gtgccgtatg caatggcctg ttctgagtgg
    16801 aaagtcccct ggctaccagt aaagccctgg cagataacct tggtgttttt atcaattaaa
    16861 atggacatta tttcccctcc actgcggcaa caacctgctg agctgcatcc gtcagacctt
    16921 ttgctgcaat aatattcagg ccgctgtcag ccagtttctt cgcgccgagt tcggcgttgt
    16981 taccttccag acgtaccacg accggtacgt taacacccac ttctgctacc gcgccgatga
    17041 taccgtcagc gatcaggtcg caacgaacga taccgccgaa gatgttaacc agaacggctt
    17101 tcactttgtc gtcagagagg atgattttga acgcttcggt tacacgttct ttggttgcgc
    17161 cgccgccaac gtcaaggaag ttagccggtt cgccgccgtg cagtttaacg atgtccatcg
    17221 tacccatcgc cagacctgcg ccgttaacca tacaaccgat gttaccgtcc agcgcaacgt
    17281 agttcagttc ccactgtgca gcctgtgctt cacgcggatc ttcctgcgac tggtcacgca
    17341 tttcgcgcag atcaggctgg cggaacagtg cgttgccgtc agcgcccagt ttgccgtcga
    17401 ggcaaatcag atcgccctgt ttggtgatga ccagcgggtt gatttcgatc aacgccaggt
    17461 cgcgctccag gaaaatggtc gccaggccca tgaagatttt ggtgaactgc tgaaccagtt
    17521 taccttccag acccagtttg aacgccagct cgcgtccctg atacggcatc gggccagtca
    17581 gcggatcaag cgcaactttg tggatcaggt gcggagtttc ttccgccact ttttcgattt
    17641 ccacgccgcc ttcggtggag gccataaaga ccacacgacg ggaactacgg tctacaacgg
    17701 caccgagata cagctcttta gcgatatcgg tcgctgcttc aaccagaatc tggttaaccg
    17761 gttggccatt ggcatctgtt tgatacgtta ccagacgctt gccgagccag ttttctgcaa
    17821 aagcacggat gtcttctttg ctgtttacaa ctttcacacc gcccgcttta ccgcggccac
    17881 cagcgtgaac ctgacatttc actacccacg ggccggcacc gatttttgaa gcggcttctt
    17941 ctgcttcgcg cggagtagta caggcataac ccaccggtgc tggtaagcca tagcgggcaa
    18001 aaagttgttt tgcctgatat tcatgtaagt tcatgtgttc tgtccatcct tcagtaatcg
    18061 ttatctttta aaccgtagac cgacacccta tccacgtcgg tctacagtgc aggtgaagct
    18121 taaactacta cacgtccagc agcagacgcg tcggatcttc cagcaactct ttgatcgtta
    18181 ccaggaagcc cacggattcg cgaccatcga tcagacggtg atcgtaggac agcgccaggt
    18241 acatcatcgg caggatctca acctgaccat tcaccgccat cggacgatct ttgatagcgt
    18301 gcatacccag aattgcgctc tgcggcgggt tgatgatcgg cgtagacatc agggaaccga
    18361 acacaccacc gttggtgatg gtgaagttac caccggtcag atcttcaacc gtcagcttgc
    18421 cgtcacggcc tttaactgcc agctctttga ttttcttctc gatgtctgcc atgccgaggg
    18481 tatcgacatc acgcagtacc ggcgtcacca ggccgcgcgg cgtggaaacc gccatgctga
    18541 cgtcgaaata gttgtggtaa accacgtcat caccgtcgat agaagcattc acttccgggt
    18601 aacgtttcag ggcttcaacc accgctttca cgtagaagga cataaagccc agacggatgc
    18661 cgtggcgttt ttcaaacgct tcaccgtact gcttacgcag atccataatc ggcttcatgt
    18721 tgacttcgtt gaacgtggtc agcatggcgg tggagttttt cgcttccagc agacgctctg
    18781 ccacacgctt acgcaggcga gtcatcggga cacgtttttc actacgtgca gccagagccg
    18841 gttgcgccgc cggagcagcc gctgccggag cagactcttt cgccggggct ttcgccagat
    18901 gtttttccac atcttcacga gtcagacgac cacccacacc ggtgccttta atggcgctgg
    18961 cgtcgagatt gtgttcagcc agcaggcgac ggatcgccgg gcttaacgca tcgttgtttt
    19021 gctcttccag agacgcctgc tggcgttgcg ccggagtgga cgctttctct tcagatttgg
    19081 cgctggtttc tttaccggcg ctgttgcctt cacgcaggcg accaaggatc tgacgagacg
    19141 ttaccgttgt accttcatct tccagaaccg catccagaat gccgtctgct gatgccggta
    19201 cttccagtac cactttgtca gtttcgattt ctaccagcac ttcatcacgt acgactgcgt
    19261 cgccgggttt tttatgccag gttgcgacgg tggcatcggc tacggattca ggcaggtcag
    19321 ggaccagaat atctacgcta ctcattgtgt atcctttatt tattcgacgt tcagcgcgtc
    19381 attaaccaga tcttgttgct gtttctggtg aacggacata taccctaccg ccggagaggc
    19441 ggaggccgtg cggcctgcat aacgcagaga agccccaaac ggaatcactt cacggaaatg
    19501 atgctggctg cagtaccatg cgccctggtt gagcggctct tcctggcacc agacaaaatc
    19561 cttgacgtga gcaaactgct gcaacacttc ctgcatcgct ttatgcggga acgggtagag
    19621 ttgctcgata cgcacaatgg cgacatcgtg ttgattgttc ttacgacgct gttccagcag
    19681 gtcgtaataa accttaccag aacacatcac tacgcgcttc acgcccttcg gatcaagctc
    19741 gtcgatttca ccgatggctg gcaggaaggt gccgttcgcc agttcttcga ggctggaaac
    19801 cgccagcgga tgacgcagca gggatttcgg cgacatcact accagcggac gacgcatccc
    19861 gcgcagcgcc tgacgacgca gcatgtggta aacctgtgcc ggggtagacg gaacgcaaac
    19921 ctgcatgttt tgctcagcac aaagttgcag ataacgttcc agacgcgcgg aggagtgctc
    19981 cggcccctgc ccttcgtaac cgtgcggcag caacatcacc agaccacaca tccggcccca
    20041 tttctgttcg ccagaagaga tgaactggtc gataaccacc tgcgcaccgt tggcgaagtc
    20101 accgaactgc gcttcccaga tggtcagagt gcgtggttct gcggtggcat aaccgtattc
    20161 aaacgccagc actgcttctt cagacagtac ggagtcccag acgcggaacg cgccctgccc
    20221 gttatggatg tgttgcaacg gcgtgtaagt ggaaccgtta gactggttgt ggatcaccgc
    20281 gtggcggtgg aagaaggtac cgcgaccgga gtcttcaccc gacaggcgaa ccggaatgcc
    20341 ttcgtcaacc agcgtggcgt aagcgaggtt ttccgcaccg ccccagtcga acagtttctc
    20401 accggcagcc atcgcctggc gatcgccata aatcttggca acgcgagact gcatttcaac
    20461 tgcttccggc accgtgctga tgcgtttcgc cagctcctgc aggcgcttca tctcaacttt
    20521 gttcgggtac tcttcgtccc attcgtggtt gaggtacggc gaccaggtga aagagtgcat
    20581 gttcatcgga cgccactctg ccactacgca atcgccagca tccagcgcat cgcggtacag
    20641 gttaaccatc tcggtggcat cttccagcgt cgccactttt tcctgctcca gcttgtcagc
    20701 gtagattttg cgcggtgtcg gatgtttttt gattttctga tacatcagcg gctgggttgc
    20761 gctcggctcg tcggcttcgt tgtggccgtg acggcggtag cacaccaggt cgatgaagac
    20821 atcacgttta aaggtgttac ggaaatcgag cgccagacgg gtcacaaagg caacggcttc
    20881 cggatcatcc gcgttaacgt ggaaaatcgg tgcctgaacc atcttaccga tatcagtaca
    20941 gtacggtgta gaacgagcat ccagtggatt agaggtggtg aaaccaacct ggttgttgat
    21001 aacgatacgt accgtaccgc caacttcata accacgcgct ttcgacatgt tcagagtttc
    21061 ctgaaccacg ccctgcccgg tcactgcggc gtcaccgtgg atggtgattg gcagcacttt
    21121 gttgctgctc ggctcatcaa gtctgtccag acgggcacga acagaaccga taactaccgg
    21181 gcttacaatc tcaaggtgag acgggttaaa cgccagcgcc aggtgcacca ggccgccatc
    21241 ggtctggaag tcagacgaga agcccatgtg gtatttcacg tcacccgtgc cgaggtgttc
    21301 tttatgttta ccggcaaact cgtcgaacaa gtcttgcggt tttttaccca gcacgttcac
    21361 cagcacgttc agacgaccac ggtgcgccat cccaagaacc acttcgcggg tgccgctgtt
    21421 gccagcgtgg cggatcatct ctttaagcat cgggattaac gcgtcaccgc cttccagcga
    21481 gaagcgtttt gcgccaggga attttgcgcc gaggtaacgt tcaaggcctt cagcggcggt
    21541 cagttcgctt aagaagcgtt ttttctcttc gctattgaaa gtcgcgcgac cagactcgat
    21601 acgctgttgg atccagcgtt tttcttcggt gctggtaatg tgcatatact cggcaccaat
    21661 cgggccacag taggtttgct tgagggcttc cagcagctcg ccgagtttca tgttttcttt
    21721 gccgctggca aatgaaccga cgttgaaggt ctcctggaag tctgcttcgg tcagatcgtg
    21781 gaaagacgga tccagatcgg ccactttatc ttgctgccac agtcccagcg gatcgagatt
    21841 cgcatgctgg tgaccacgga agcggtatgc gttaatgagc tgcaggactt taacctgctt
    21901 cacattggtg tcagggtcgg agatcgttga agagtaacgt gaagcgtctt tcgccaggcg
    21961 gcggaaatat tcacgcgttt gagagtggaa ttgatccggt ttgactcccg taccaggtaa
    22021 ctgctggaac gtcgaacgcc agttagcgtc aaccgagtca ggatcggtta agaagtcttc
    22081 atagagctgt tctatccagc tctggtttgc gccagagagg taagaagagt ccaaccaggc
    22141 tttcaaagcg ctgttctgca tcgtgatccc ttaagcatct tttttatgct tacttcgccg
    22201 tggatactac cacgcacagt gatgtgcgtg ccggggttca cttgcgagct cttgtctatg
    22261 tagtggctcg cgaaggaacc tttaaaaact gtcttagtgt caggggcagt ttttaaagat
    22321 ttcctgcatt tctttgttac gcctgatgcg ctgcgcttat caggcctacg ggtaatgact
    22381 tgtaggccgg ataaggcgtt cacgccgcat ccggcactgg ttgcctgatg cgacgcttgc
    22441 gcgtcttatc aggcctacgg tttacgcatt acgttgcaac aacatcgact taatatggcc
    22501 gatggcgcgc gtcgggttca gccccttcgg acatacactg acgcagttca tgatgctgtg
    22561 acagcggaat acgctgaatg catcgctcaa accgtcgagg cggctgtcag tctcggtatc
    22621 acggctatcg atcaggaaac gatatgccgc taacaagcct gccgggccga taaacttatc
    22681 gggattccac cagaaagacg gacaagaggt tgaacaacat gcgcagagaa tacattcata
    22741 cagcccgtcg agtttttcgc gctgctctgg catctgtaaa tgctcgcgag ctggcggatt
    22801 ttgtccatta ttcaacaggt aaggcttaat tttctcatat tgcgcataga attgtcccat
    22861 gtctaccacc aaatcgcgga tcaccggtaa acctggcagc gggcgaatca caatcttctt
    22921 gcccggctgg ttgagtgccg aaatcggggt aatacaggcc agaccattct tgccgttcat
    22981 gttcagaccg tcggaaccgc acacaccttc acggcaggag cggcggaacg acaggctggg
    23041 atctttctct ttcagctgga taagcgcatc cagcagcatc atgtcgcgac cttcttccgc
    23101 ttccaggggg taatcctgca tacgcggagc atcatcaaca tccgggttat agcgataaat
    23161 tgaaaactcg agtctcattt tcctgtctcc gcattagtaa gtacgaatct tcggcgggaa
    23221 tgccgggcgc agtttcggtt ccatgttgac gcttcggcgc gtcatggatt ccgactctgg
    23281 cagatacagg gagtggcaca gccagttttc atcatcacga tccgggaagt cgaagcggct
    23341 atgtgcgcca cggctttcgg tacggaagtt ggcagaaaca gccgttgcat acgccgtttc
    23401 catcaggtta tccagttcca agcactcaac gcgctgggtg ttgaactcgc tggaagtgtc
    23461 atccagacgg gcatttttca gacgctcgcg gatcactttc aactgctcaa gccctttcgc
    23521 catcgcatca ccttcacgga agaccgagaa gttatgctgc atacattctt gcagcgcttt
    23581 acgaatcgcc accggatctt caccgttacg attattgttc cagcggtgca gacgatccag
    23641 agatgcttca acatcagact cgctggcatc gcgcaatgcg ccctgttcgg cgatagactc
    23701 ttgcagatgc agacctgccg cacgaccaaa gaccaccagg tccagcagcg agttgccgcc
    23761 cagacggtta gcgccgtgta ccgatacaca agcgatttca ccaacggcaa acagccccgg
    23821 aacaaccaca tcttcgcctt tctcgttcac cgtcagcgcc tgaccggtca ctttggtcgg
    23881 aataccgccc atcatgtagt gacaggttgg gataaccgga atcggctctt tcaccggatc
    23941 gacgtgagca aaggtacggg aaagctccag gatacccggc agacgggatt cgagaacttc
    24001 tttacccagg tgatcgagtt tcagtttcgc gtgtggcccc cacggaccat cacagccgcg
    24061 accttcacgg atttcaatca tgatggaacg cgccaccaca tcacggcccg ccaggtcttt
    24121 ggcgttcggc gcataacgct ccataaagcg ttcgccatgt ttgttcagca gataaccgcc
    24181 ttcaccacgg caaccttcgg tgaccagtac gcccgcaccg gcaatgccgg tcgggtggaa
    24241 ttgccacatt tccatatcct gcaccggtac gcctgcgcgg atagccatgc cgacaccgtc
    24301 gccagtgtta atgtgggcgt tagtggtgga ctgataaata cgccctgccc cgccagtcgc
    24361 cagcacggta gcgcgggctt tgaaataaac cacttcaccg gtttcgatgc acagtgcggt
    24421 acaacccacc accgcgccat cctggttttt caccagatcc agcgcatacc actcggagaa
    24481 aatggtggtg tggtttttca ggttctgctg ataaagcgtg tgcaacagtg cgtgaccggt
    24541 acggtcagcc gccgccgcag tgcgtgccgc ctgctcgccg ccgaagtttt tcgactgacc
    24601 gccaaacgga cgttgataga tacgaccatc atcaagacgc gagaacggca ggcccatgtg
    24661 ttcgagttcc agaatcgctt ccggcccggt tttacacata tattcaatcg cgtcctggtc
    24721 accgatatag tccgaccctt ttacggtgtc gtacatatgc cattcccagt tatcttcatg
    24781 ggtattaccc agcgcaacgg taatgccgcc ttgcgcagaa acggtatggg aacgggtcgg
    24841 gaagacttta gagagcagcg cacaggtctg gccgctctgg gaaatttgca gcgccgcgcg
    24901 catacctgcg ccaccggcac caatcacaac tgcatcaaat tctctgactg gcaatttcat
    24961 cacacacccc acaccacaac gaatccataa atcacgtaaa ccaccagtgc aacgacaatc
    25021 accagttgca gcatcaggcg caatgccagc ggtttaacgt agtcggtcaa cacctgccac
    25081 atgccgatcc aggcatgaat caagatagaa aacagcgcca gcagggtgaa cactttggtg
    25141 aacgcagagg cgaagaaacc gatccagact tcatatgtca gttcgccact ggtggcgaaa
    25201 aaaccgacca tataaatgat gtagagcgtc aggacgatag cggtagcgcg aacgaggatg
    25261 aaatcatgta cgccattgcg tcctaatgcg gaggcgttgc ttaccatacg aggactcctg
    25321 cgagaagtga aagcacgaca gtaataacaa aggagatttt ggcggagcgt ttacccgctt
    25381 cgaatgtttc ttccagatag ccaaaatcca tcatcatgtg gcgaatacct acgacgacgt
    25441 gatacgccag agcggtaagg atgccccaca tgataaattt gacgaagaag ctgcccataa
    25501 tcgcggaagc ttgctcgaaa ccttcagggg aagagaggct ggtacccaga agccacagca
    25561 ggatgcccac tgcaacaaag gtgatcacac cggaaacgcg atggagaatg gacgctatcg
    25621 ccgtgatggg gaaccggatg gtctgtaggt ccagattaac aggtctttgt tttttcacat
    25681 ttcttatcat gaataacgcc cacatgctgt tcttattatt ccctggggac tacgggcaca
    25741 gaggttaact ttctgttacc tggagacgtc gggatttcct tcctccggtc tgcttgcggg
    25801 tcagacagcg tcctttctat aactgcgcgt catgcaaaac actgcttcca gatgcgaaaa
    25861 cgacacgtta caacgctggg tggctcggga ttgcagggtg ttccggagac ctggcggcag
    25921 tataggctgt tcacaaaatc attacaatta acctacatat agtttgtcgg gttttatcct
    25981 gaacagtgat ccaggtcacg ataacaacat ttatttaatt tttaatcatc taatttgaca
    26041 atcattcaac aaagttgtta caaacattgc caggaaaagc atataatgcg taaaagttat
    26101 gaagtcggta tttcacctaa gattaactta tgtaacagtg tggaagtatt gaccaattca
    26161 ttcgggacag ttattagtgg tagacaagtt taataattcg gattgctaag tacttgattc
    26221 gccatttatt cgtcatcaat ggatccttta cctgcaagcg cccagagctc tgtacccagg
    26281 ttttcccctc tttcacagag cggcgagcca aataacaaac gggtaaagcc aggttgatgt
    26341 gcgaaggcaa atttaagttc cggcagtctt acgcaataag gcgctaagga gaccttaaat
    26401 ggctgataca aaagcaaaac tcaccctcaa cggggacaca gctgttgaac tggatgtgct
    26461 gaaaggcacg ctgggtcaag atgttattga tatccgtact ctcggttcaa aaggtgtgtt
    26521 cacctttgac ccaggcttca cttcaaccgc atcctgcgaa tctaaaatta cttttattga
    26581 tggtgatgaa ggtattttgc tgcaccgcgg tttcccgatc gatcagctgg cgaccgattc
    26641 taactacctg gaagtttgtt acatcctgct gaatggtgaa aaaccgactc aggaacagta
    26701 tgacgaattt aaaactacgg tgacccgtca taccatgatc cacgagcaga ttacccgtct
    26761 gttccatgct ttccgtcgcg actcgcatcc aatggcagtc atgtgtggta ttaccggcgc
    26821 gctggcggcg ttctatcacg actcgctgga tgttaacaat cctcgtcacc gtgaaattgc
    26881 cgcgttccgc ctgctgtcga aaatgccgac catggccgcg atgtgttaca agtattccat
    26941 tggtcagcca tttgtttatc cgcgcaacga tctctcctat gccggtaact tcctgaatat
    27001 gatgttctcc acgccgtgcg aaccgtatga agttaatccg attctggaac gtgctatgga
    27061 ccgtattctg atcctgcacg ctgaccatga acagaacgcc tctacctcca ccgtgcgtac
    27121 cgctggctct tcgggtgcga acccgtttgc ctgtatcgca gcaggtattg cttcactgtg
    27181 gggacctgcg cacggtggtg ctaacgaagc ggcgctgaaa atgctggaag aaattagctc
    27241 cgttaaacac attccggaat ttgttcgtcg tgcgaaagat aaaaatgatt ctttccgcct
    27301 gatgggcttc ggtcaccgcg tgtacaaaaa ttacgacccg cgcgccaccg taatgcgtga
    27361 aacctgccat gaagttctga aagagctggg caccaaagat gacctgctgg aagtggctat
    27421 ggagctggaa aacatcgcgc tgaacgaccc gtactttatc gagaagaaac tgtacccgaa
    27481 cgtcgatttc tactctggta tcatcctgaa agcgatgggt attccgtctt ccatgttcac
    27541 cgtcattttc gcaatggcac gtaccgttgg ctggatcgcc cactggagcg aaatgcacag
    27601 tgacggtatg aagattgccc gtccgcgtca gctgtataca ggatatgaaa aacgcgactt
    27661 taaaagcgat atcaagcgtt aatggttgat tgctaagttg taaatatttt aacccgccgt
    27721 tcaaatggcg ggttaatttt tatatgccca aacacaaaaa attgtaaaaa taaaatccac
    27781 tatcggacat tcatatattc taaaaaaaat atatcggtat aaattataaa caaatcgatg
    27841 atttttaatc atctatcttc ctctcagtta catgaataaa ccaccaaata tagcgcaccc
    27901 ctataatttc caatacttta ttcatcaaac atttaattca taccatcaat taattacttt
    27961 tacatttaat aaaattaata atatttttaa gaaaaactaa aaacattcga caatcaaata
    28021 agatattctt gcaccgatat acttttttct tttctgttaa aggactcagt tatgtttaaa
    28081 ggacaaaaaa cattggccgc actggccgta tctctgctgt tcacagcacc tgtttatgcg
    28141 gctgatgaag gatccggtga aattcacttt aaaggtgaag ttattgaagc accgtgtgaa
    28201 atacatcagg atgatattga taaagaggtt gaactcggtc aggtgaccac cagccacatt
    28261 aatcagtcac atcacagcga tgccgttgct gtcgacctgc gcttagtcaa ctgtgatctg
    28321 gaaaactcca gcaacggttc cggtggcaag atttctaaag ttgcagtcac ctttgatagc
    28381 tcagcgaaaa ccaccggcgc agatccgatt ctcaacaaca ccagcacggg tgaagccacc
    28441 ggcgtcggcg tacgtttaat gaataaagat caaagcaata tcgttttagg caccgctact
    28501 ccagatatcg acctggctcc gacctccagc gaacaaacgc tgaatttctt tgcctggatg
    28561 gaacaaattg atcaggctac acctgtaacg ccaggcgcag ttacggcgaa tgcaacctac
    28621 gtgctggatt ataaataagt attactttgg cggcgctatc gccgcctttc ataaaatatt
    28681 tatcaggtat ggacaccgtg aatatttatc gactctcttt tgtatcctgc ctggtcgtgg
    28741 cgatgccttg cgcattggcg gtcgaattca accttaatgt tctcgataaa tcgatgcgcg
    28801 accgcattga tatttcatta ttaaaggaaa aaggagtcat tgctcccggt gagtattttg
    28861 ttagcgttgc ggtaaataat aaccaaatca gtaacgggca aaagattaac tggcacaaaa
    28921 atgacgataa aaccattccg tgcatcaatg atttactggt cgataaattt ggcttaaaac
    28981 ctgaagtccg tcagtcgtta ccattgataa atcagtgcgt cgattttagc tcccgacctg
    29041 aaatgctctt caatttcgat caagccaatc agcaactgaa tattagtatt ccgcaagcct
    29101 ggctggcgtg gcactcagaa aactggaccc caccctccac atggaaagaa ggtgtcgccg
    29161 gtatcctgat ggattacaac ttgtttgcca gcagctaccg cccacaggac ggcagcagca
    29221 gcactaacct gaacgcctac ggtaccaccg gaattaacgc cggggcatgg cgcttacgta
    29281 gtgattatca gttgaatcag actgatagcg atgataacca tgaacagtca ggcggaatat
    29341 cgcgcaccta tctttttcgt ccattaccgc aattaggctc taaattaacc ctcggcgaaa
    29401 cggattttag ttccaatatt ttcgacggtt tttcttatac cggcgcggca ctggcaagtg
    29461 acgagcgaat gttgccatgg gaactacgcg gctacgcccc acaaattagc ggtattgcac
    29521 agaccaatgc cacggtgacg atcagtcaat caggccgcgt catttaccag aaaaaagtcc
    29581 caccaggccc atttatcatt gacgacctta atcagtctgt tcagggcaca ctggatgtca
    29641 aagtgacgga agaagatggt cgggtgaaca atttccaggt ttcggcagca tcgacgccct
    29701 tcctgactcg tcagggacag gttcgctata aactggccgc gggtcagcca cggccctcca
    29761 tgtcacatca aactgaaaat gaaacctttt ttagcaatga agtttcctgg gggatgctgt
    29821 caaacacctc gctgtacggc ggcctgctgc tttctggtga tgactaccat tctgccgcaa
    29881 tgggtattgg gcaaaatatg ctgtggcttg gtgcgctgtc gtttgatgtc acgtgggcca
    29941 gtagccattt tgatactcag caggacgagc ggggcttaag ctaccgtttt aattacagca
    30001 aacaagtgga tgccactaac agcacgattt ccctcgccgc ttatcgtttc tccgatcgtc
    30061 attttcacag ctacgccaac tatctggatc acaaatacaa cgacagcgat gcgcaggacg
    30121 aaaaacagac gatcagctta ttgtgggcca accgattacc ccactaaacc tcaatcttta
    30181 cgccaacctg ctacatcaaa cctggtggaa tgcagacgcc tccacgaccg ccaacatcac
    30241 ggcaggtttt aatgttgata ttggtgactg gagagatatc tctatttcga cgtcattcaa
    30301 tacaacccat tacgaagata aagatcacga caaccagatt tacctgtcga tttcgctccc
    30361 cttcggtaac ggtggtcggg ttggctatga catgcaaaac agtagccaca gcaccacaca
    30421 ccgcatgtcg tggaacgata cgctggatga acgtaatagc tggggcatgt ctgccggact
    30481 gcaatccgac cgtcctgaca atggagccca ggtgagcggt aactatcagc acctgagttc
    30541 agcgggtgag tgggatattt ctggtaccta tgccgccaat gattacagtt ccgtcagcag
    30601 cagctggagc ggttctttca ccgcaaccca atatggtgca gcgtttcatc gccgcagctc
    30661 caccaatgaa ccacgcctga tggtcagcac cgatggcgtg gcagatattc cggttcaggg
    30721 caatctcgac tacaccaacc attttggcat tgcggtggtg ccgttgattt ccagttacca
    30781 gccttccacc gtggcggtga acatgaatga cttacccgac ggcgtaacag ttgcagaaaa
    30841 cgttatcaaa gaaacgtgga ttgaaggcgc gataggttac aaatcactgg cttcccgttc
    30901 cggtaaagac gttaacgtca tcattcgcaa cgccagcggt cagtttcctc ccctcggagc
    30961 ggatatccgc caggatgaca gcggcattag cgtggggatg gttggcgagg aaggacatgc
    31021 ctggttaagc ggagtcgctg aaaatcaaaa gtttaccgtg gtctggggtg atagccagca
    31081 ttgctcgctc catcttcctg aacatatgga agacaccgca aatcgcctga ttttaccttg
    31141 tcattaatta aggaaatgaa aatgacattt atgaaaggac tacccctttt gttgttggtt
    31201 gccagtctgt gcagccacgc tgcactacaa cccgatcgca ctcgtattgt gtttaacgct
    31261 aatgataaag ctaccagtct gcgggtggat aatcgtagcg ataaactccc ctacctcgct
    31321 tattcctggc tcgaaaatga aaaaggggag aaaagcgatg atcttctggt cgccctgccg
    31381 ccgatacaac gtctggaacc gaaagcaaca acgcaggtac ggatagtaaa gcaagcctcg
    31441 accacaaaat taccgggcga tcgcgaaacg ctatttttct acaatatgcg tgaaattcct
    31501 cccgccccgg aaaaaaacag cgaccatgcc gttctgcaag tcgccattca gagtcgtatc
    31561 aaggtgttct ggcgaccggc tgcattacgc aagaaagcag gagaaaaggt cgaactgcag
    31621 ttacaggtca gccagcaggg caaccaactg acactgaaaa atcctaccgc gtattatctg
    31681 accattgcct acctgggacg aaatgaaaaa ggcgttctcc ccggcttcaa aaccgtgatg
    31741 gttgcgcctt ttagtacggt caacacaaac accggaaatt atagcggcag ccagttttat
    31801 ctcggttata tggatgatta cggcgcgtta cgcatgacga cgctcaactg cagcggacaa
    31861 tgccatttac aagccgtgga ggcgaagaaa tgagtgctgg caagggagtg ttgctcgtca
    31921 tctgtctgtt atttctgccg ttgaagtctg cgcttgccct gaactgctac tttggttcat
    31981 ccggtggctc ggtagaaaaa tcagaagcga ttcaaccttt tgctgtacca ggcaatgcca
    32041 aactcggcga taagatttgg gaatctgacg atattaaaat tcccgtctat tgtgacaaca
    32101 ataccaacgg caattttgaa agtgagcatg tttatgcctg ggtaaacccc tacccggggg
    32161 tgcaggatcg ctattatcaa ctcggcgtaa cttacaatgg cgttgattat gacgccaacc
    32221 agggaaaaag ccgcatcgac actaaccagt gtattgacag taagaacatt gacatttata
    32281 cccctgagca gattatcgcg atgggatggc agaacaaaat ttgctcgggt gatcccgcta
    32341 atattcacat gtcgcgtacc tttctcgctc gcatgcggtt atacgtcaaa atacgagaaa
    32401 tgccgccgca tgattatcaa agtacgctta gcgactatat cgtcgtgcaa tttgacggtg
    32461 ccggtagcgt taacgaagac cctactgccc aaaacctgaa atatcatatt actggtctgg
    32521 aaaacattcg cgtgctggat tgcagcgtca atttttccat ttcaccggaa acacaggtga
    32581 ttgattttgg taaatttaat ttgctggata ttcgtcgcca cacaatgtcg aaaacattca
    32641 gtattaagac gacaaaatca caaaatgacc aatgcaccga cggatttaaa gtcagttcct
    32701 ccttttatac cgaagaaacg ttggttgaag aagataaagc attgttgatt ggtaatggtc
    32761 tgaagctacg tttgctggat gaaaacgcct cgccttatac cttcaataaa tatgctgaat
    32821 acgccgattt caccagcgac atgttggtct acgaaaaaac ctatacagct gaactttcgt
    32881 ccattgcagg tacccccatc gaggctgggc ccttcgatac agtggtgctt tttaaaatta
    32941 actataactg atttgccatg atgccctttg cagccggaaa gggcatctct ttcagaaaat
    33001 tgctgatttt ccccttttcc ttgattgctg ttattttttc ctggcccggc tttctttatg
    33061 ctgtatcgat acaccaaaga gaaaataatg agggagcgaa ggatgccagt tttgcagtgg
    33121 ggaatgttat gtgtgctatc acttctcctt tctattggtt tcctcgcggt acatctcccg
    33181 gcggcgctat tacttgggcc gatgatcgcc gggatcatct tcagtatgcg cggaattact
    33241 ctgcaacttc cccgctccgc ttttctcgcc gcgcaggcca ttcttggctg catgattgcg
    33301 caaaacctca ctggttcgat tctcaccacg ctggccgcca actggccgat cgtgcttgct
    33361 attttactgg tgacgctgct ttccagcgcc atcgtgggtt ggttattggt gcgctatagc
    33421 tcactgcctg gaaataccgg tgcctggggt tcttctccag gcggcgcagc ggcaatggtc
    33481 gctatggcgc aagattacgg cgcagatatt cgcctggttg catttatgca atatctgcga
    33541 gtgctgttcg tcgcgggggc cgctgttctg gtcacccgca tgatgctggg tgataacgct
    33601 gaagcggtta accagcaaat cgtctggttc ccgccagtga gcattaatct cctgcttacg
    33661 attttgctcg cagtcgttgc cggtacggcg ggatgcatgc tgcgtcttcc ttccggcacg
    33721 atgctcatcc cgatgctggc gggcgcagtg ctccagtctg gtcagctcat caccatcgaa
    33781 ctaccggaat ggctgctggc gatggcgtat atggcaattg gctggcggat tggtcttggt
    33841 ttcgataaac aaatcttact gcgggcacta cgcccgctac cgcaaatcct gctgtcgatt
    33901 tttgctctgc tggctatttg tgcgggtatg gcgtgggggc tgacccggtt tatgcacatt
    33961 gattttatga ctgcctacct cgccaccagt cccggcgggc ttgataccgt ggcggtcatc
    34021 gccgcaggga gcaatgccga tatggcgctt attatggcga tgcaaaccct gcgcctgttc
    34081 agtattttgc tgacggggcc agccattgcg cggtttattt caacctatgc gccgaagcgg
    34141 tcggcctagt gctggcagcc agggcaccag taaaacgggc gagatgacag cgtggttttc
    34201 tcaatgatgc tgccacagcg ttcgcacggt tcgccatcac gatgaaaaac cttaaagcga
    34261 aacagcgccc catgatgctt attctcatcc accagccccc tcgtagcgta ggaaaatcga
    34321 ggaatatcca gtaacgcgtg tgcgagtgca tccagttgcg ccgcattgag atctttcgct
    34381 ttatgatttc cagtcaaccc aacctgccag aggatctcca cccgcaaata attgccaagc
    34441 ccagccagaa acgcctgatc gagcagtaat cctgcaaact gacggttacg aaagcgcggt
    34501 gacaataacc gttcctttac cacctccggc gtcagattcg gatccagcac atcgggacca
    34561 acgcgttgta aaaacggatg cgtggtcagt tgttccggcc tcaacatctc aatatccgag
    34621 gcgctataaa gcagaatggt tttgtcagcc gtttgcagtt ttacccgcaa tactcgcgtg
    34681 gtctgcggct cttcgccggt atcaaccacg cgccagacgc cgtaaagctg attatggctg
    34741 tagagcgtta agtcgttgga aaaatgagtt aacaacgcct taccacgcgt ttccacatgg
    34801 gtaacgtgtt gaccgataag ttgtgattga taagttttta actgcgggaa ggcaaaccag
    34861 acatcagtta gtggtttgcc tttgatcgcc gcctccaggt tatccgctgc acggcggatc
    34921 tccgggcctt caggcatggt gatatccttt tttcattctt tattaatcgg ggttatgctg
    34981 cgacaacaat ctccttttcg gcaaatgtag agcgtagtcg gcgggcgaac gccagtgcgt
    35041 gctcgccgtc gccatgcagg cagactgttt gcgccgtgac cgttgcccat tcgccggtga
    35101 tacttttgac tctgccgtgt tgcaccatct ctagcgtttg cgccaacgcc tgttcttcgt
    35161 tttcaatcaa cgcgcctcgc tgacttcgcg gcaccagcga gccgtcagcc tgataaccgc
    35221 gatcggcaaa cacttcctcg cgcgttgtca ggccatattg cttgcctgca cgaatcagct
    35281 cgcttcctgc cagcccaacg agaatcaatg ccggatcgca agcgtatacc gctctggcga
    35341 tggcgtctgc cagttgtgct tctttcgccg cctggttgta caacatgccg tgcggtttga
    35401 catgacgcat tacgccgcct tgcgcacggg caatcgttgc cagcgcgcca atttgataca
    35461 gcgtctgggc gtaaacggtt tctggcggca gctgcatggc gctgcgacca aaattttccc
    35521 tgtcgggaaa actcgggtga gcgccaatcg cgacaccatt ttttattgct tcacgcacgc
    35581 aagcctgcat gatttgcgca tcgcctgcat gaaatccaca ggcaatattg gcagaggaaa
    35641 ccagcgttaa tagctctgcg tcgctggcgc agccttcgcc cagatcggcg ttcaggtcaa
    35701 ttttcattgt gcagccgcca cactaattgt tcgaaataac gttgctgatc ttgccgcgct
    35761 ttcagtgcct cttccagtga acactggaca aaatgaatcg gctgaccgag cggaatttgc
    35821 gccagatggt acatatcagc ctcaatgata caggcaatac gcgggtaacc atcggtggtc
    35881 tgtgcgtcgt tcatcaacac aatcggctgc ccgttatgtg gcacctgcac cacgcccggt
    35941 aacaaaccgt gagataacag ttcgcgatcg gtggtgcgtt ttaaaatttg cccctgtaag
    36001 cgatagccca tgcggttact ttgcgagcta agctgccagg gcgaacgcca gaatgcatcc
    36061 tgcgaggcgc gatcgaactc atgatattcc ggccccggca aggcgcgaat gcggttgccc
    36121 cacagcagct gtttaacgcc ctgcgcttcc ataaaatcac gcttcgattt gccaatcggg
    36181 agtcggtcac catccttcag taaacggcct tccagcccgc caatccccac tttgagatcg
    36241 gtgctgcatg accccattac cggcggaaca tcaataccac ccgcgaccgc cagataactg
    36301 cgcatcccgt gctgcgggcg tttaagcgtt aaacgctggc ctgctttcat cggcaatcgc
    36361 cagccggtcc agacggcatt atcatccagc cgcgcttcgc aaccggcacc cgtcagagca
    36421 aaccacccat cagtttcgaa ctcaaccgtt aactgaccga gcgtgatctc cagcgcgggg
    36481 gcattggcgt cattacccac cagcaggtta gcaatgcgta acgcaggcat atccagtgcg
    36541 ccgcagtggc tgattcccga ctggcgaaaa ccgtgacgac cgccatcctg cacagtggta
    36601 tacatgcccg cacgaataat cttcagcata ctccctcctt ctgcggtaca aagcgcacgc
    36661 tgtctcccgg acgtaataag atgggttcgt cacgcgccgg atcaaacagg ctgagtgagg
    36721 tatgaccaat caactgccag ccacccggcg ttgccagcgg ataaacacca gtctgcggcc
    36781 cgccgatccc gacagaacct gccggaacga gcaagcgcgg ttcagcgcgc cgtggcgtgt
    36841 gtagttgttc cggcaaactc ccgagatacg ggaagcccgg ttgaaaacct aaaaaccaga
    36901 ccacgtattc cacggaggag tgcaattcaa caacctgttt ttcgctaaac ccgcaatgcg
    36961 ccgcgaccac cgccaaatcc ggtccgcctg caccaccgta aaccaccgga atttcaataa
    37021 agcgagactc aggttccagg gcctcgctct cctcccacca gcgttgcaaa cgctctatgg
    37081 catccagcgc cagcgactca ggattacgca aaatcaccgt gatattgttc atgccgggaa
    37141 tggcttcaac cacattcggc atatccacca gacgctgcgc cagtcgccag atccgtttct
    37201 ggctagccag cgtcaccggc ggttccagtt ccagcactac cgccgtttca cctatcagat
    37261 aacaacgcgc tcgttgcact ttcgatccct ctgattattc gttatgcagg attaggaata
    37321 tcaataaagg tcacatcaag atcggtattt tcattcagcc actcgctcaa tgcgcgaata
    37381 ccaccacgtt cagtggcatg gtgacctgca gcataaaaat gcaacccctg ctcgcgggct
    37441 gaatgaatgg tctgttcaga aacttcgcca gtaataaaag catccacgcc aaaacgcgcg
    37501 gcgctatcga taaaactttg cccgccgccc gtgcaccagg cgacgcgctg gaccacctca
    37561 ggtccggtat cgccacacca taacggctta cgtcccagac gcgcttcaat ccaggaggct
    37621 agttccagtc ccggcacagg catggtcagc tctccccacg gcaccaacgg ctcaatttcg
    37681 cccatgaccg tgatccccag taacgccgcc agctgtgcgt tattgcccag ctcaggatgt
    37741 acgtcaagcg gcaaatgcca gccatacagg ttgatatcat tcgccagcaa cgtttttaaa
    37801 cggttacgct tcattccgcg aatgaccgga gactctcctt tccagaagta gccatgatgc
    37861 acaatgaccg catccgcgcc cagacgcact gcctcatcga gcaaagcctg gctggcggtg
    37921 acaccggtaa caattttttg caccgtctct ttgccttcta cctgcaaacc attcggcgcg
    37981 taatcactaa tcgccgcgct gttcagtttt tcgttaatca gttgttccag ttcggtgttt
    38041 ttcatcttcg cgccttccgt attctgatag ctacaccgtt aacatagcgt tacctcgcct
    38101 gttcgtcgat agccaatctt ttgacatatt aagaaacaaa cccgacgcca aaagacgcaa
    38161 attgtttata tcatcacgct tcagctaatg tatatttatg caatcagaca gcatattgca
    38221 taaataatca gatctgtatc aaagttttaa ctttaaggaa acacaagcac gccgcgcaac
    38281 tattacgtga cgttgcagtc aattgtggaa ttattatgaa taaacacgca tcacaaccgc
    38341 gcactattta ctatgtcgtt gcgctgcaaa tctgggaata ttttagcttt tacggcatgc
    38401 gtgccctgct gattctctat ctcaccaatc aactaaaata caacgatact cacgcctacg
    38461 agttatttag cgcctactgt tcgctggtgt atgtcacgcc aatcctcggt ggctttttgg
    38521 cggataaagt tctcggcaat cgcatggcgg tgatgctggg ggcgttgttg atggcgatcg
    38581 gtcatgtggt gctgggtgcc agtgagatcc atccgtcatt cctctatctg tccctggcga
    38641 ttatcgtttg tggctatggt ctgtttaaat ccaatgtcag ttgcctgctc ggcgagctgt
    38701 atgagccaac cgatccgcgt cgtgatggcg gtttctcgct gatgtatgca gcgggtaacg
    38761 tggggtctat tatcgcacct atcgcctgtg gttacgccca ggaagagtac agctgggcga
    38821 tgggctttgg cctggcggcg gtgggtatga tcgcgggtct ggtcattttc ttatgtggca
    38881 atcgtcattt cacacatacc cgcggcgtta ataaaaaagt actgcgtgca acaaactttc
    38941 tcctgcctaa ctggggatgg ctgctggttc tgctggtggc aacgcctgcg ctgattaccg
    39001 tactgttctg gaaagagtgg tcggtatacg ccttaattgt cgcgactatt attggcctgg
    39061 gtgtactggc aaaaatttat cgcaaagcag aaaaccagaa acagcgtaag gagctggggc
    39121 tgattgtgac gctcaccttc ttcagtatgt tgttctgggc cttcgcacaa cagggcggca
    39181 gctcaattag cctttatatc gaccgcttcg ttaaccgcga tatgtttggt tataccgttc
    39241 cgactgctat gttccagtcg attaatgcct tcgcagttat gctatgcggt gtgttcctgg
    39301 cgtgggtggt taaagaaagc gtcgcaggta atcgtaccgt gcgcatctgg ggaaaatttg
    39361 ctcttggcct tggcctgatg agcgccggat tctgcattct gaccttaagc gcccgctggt
    39421 ccgcaatgta tggtcactct tctctgccac tgatggtgtt aggcctcgcg gtgatgggct
    39481 ttgcggaact gtttatcgac ccggttgcca tgtcgcaaat tacgcgcatt gaaattccgg
    39541 gcgtgaccgg cgtattaacc ggcatctaca tgctgctttc tggcgcgatc gcgaactatc
    39601 tggcgggcgt gattgccgat cagacatcgc aggcttcgtt tgatgcttcc ggggcgatca
    39661 actactccat caatgcatat attgaagtat ttgatcaaat tacctggggc gcactggcgt
    39721 gtgtaggact ggtactgatg atttggctgt atcaggcgct gaaattcaga aaccgcgcgc
    39781 tggcgctgga gtcataatag gccagcaccg atgcctgatg cgacgcttga cgcgtcttat
    39841 ca
//
LOCUS       DAJVQG010000017        39407 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJVQG010000017 DAJVQG010000000
VERSION     DAJVQG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.017"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..266
                     /locus_tag="OQM83_000844"
     CDS             <1..266
                     /locus_tag="OQM83_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCS1519171.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            360..758
                     /gene="fadM"
                     /locus_tag="OQM83_000845"
     CDS             360..758
                     /gene="fadM"
                     /locus_tag="OQM83_000845"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCS1519172.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(810..1505)
                     /gene="queC"
                     /locus_tag="OQM83_000846"
     CDS             complement(810..1505)
                     /gene="queC"
                     /locus_tag="OQM83_000846"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCS1519173.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1570..3270)
                     /locus_tag="OQM83_000847"
     CDS             complement(1570..3270)
                     /locus_tag="OQM83_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HCS1519174.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3370..4188
                     /gene="cof"
                     /locus_tag="OQM83_000848"
     CDS             3370..4188
                     /gene="cof"
                     /locus_tag="OQM83_000848"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCS1519175.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4340..4798
                     /gene="decR"
                     /locus_tag="OQM83_000849"
     CDS             4340..4798
                     /gene="decR"
                     /locus_tag="OQM83_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCS1519176.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            4828..6600
                     /locus_tag="OQM83_000850"
     CDS             4828..6600
                     /locus_tag="OQM83_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCS1519177.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6593..8374
                     /locus_tag="OQM83_000851"
     CDS             6593..8374
                     /locus_tag="OQM83_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCS1519178.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8555..8893
                     /gene="glnK"
                     /locus_tag="OQM83_000852"
     CDS             8555..8893
                     /gene="glnK"
                     /locus_tag="OQM83_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCS1519179.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8923..10209
                     /gene="amtB"
                     /locus_tag="OQM83_000853"
     CDS             8923..10209
                     /gene="amtB"
                     /locus_tag="OQM83_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCS1519180.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="OQM83_000854"
     CDS             complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="OQM83_000854"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCS1519181.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11336..11908
                     /locus_tag="OQM83_000855"
     CDS             11336..11908
                     /locus_tag="OQM83_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCS1519182.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11939..12250)
                     /locus_tag="OQM83_000856"
     CDS             complement(11939..12250)
                     /locus_tag="OQM83_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCS1519183.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12413..12509
                     /gene="ffs"
                     /locus_tag="OQM83_000857"
     ncRNA           12413..12509
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OQM83_000857"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12629..12982
                     /locus_tag="OQM83_000858"
     CDS             12629..12982
                     /locus_tag="OQM83_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCS1519184.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="OQM83_000859"
                     /pseudo
     CDS             complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="OQM83_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14736..15206)
                     /locus_tag="OQM83_000860"
     CDS             complement(14736..15206)
                     /locus_tag="OQM83_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCS1519185.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15322..15873)
                     /gene="maa"
                     /locus_tag="OQM83_000861"
     CDS             complement(15322..15873)
                     /gene="maa"
                     /locus_tag="OQM83_000861"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCS1519186.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16044..16262)
                     /gene="hha"
                     /locus_tag="OQM83_000862"
     CDS             complement(16044..16262)
                     /gene="hha"
                     /locus_tag="OQM83_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCS1519187.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="OQM83_000863"
     CDS             complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="OQM83_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCS1519188.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIETFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(17208..20357)
                     /locus_tag="OQM83_000864"
     CDS             complement(17208..20357)
                     /locus_tag="OQM83_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCS1519189.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="OQM83_000865"
     CDS             complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="OQM83_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCS1519190.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            21715..22362
                     /gene="acrR"
                     /locus_tag="OQM83_000866"
     CDS             21715..22362
                     /gene="acrR"
                     /locus_tag="OQM83_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCS1519191.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            22490..25852
                     /gene="mscK"
                     /locus_tag="OQM83_000867"
     CDS             22490..25852
                     /gene="mscK"
                     /locus_tag="OQM83_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCS1519192.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSHLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGISWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="OQM83_000868"
     CDS             complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="OQM83_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCS1519193.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDKAEK"
     gene            complement(26239..26766)
                     /gene="priC"
                     /locus_tag="OQM83_000869"
     CDS             complement(26239..26766)
                     /gene="priC"
                     /locus_tag="OQM83_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCS1519194.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            26836..27213
                     /locus_tag="OQM83_000870"
     CDS             26836..27213
                     /locus_tag="OQM83_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCS1519195.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            27366..27917
                     /gene="apt"
                     /locus_tag="OQM83_000871"
     CDS             27366..27917
                     /gene="apt"
                     /locus_tag="OQM83_000871"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCS1519196.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            28046..29971
                     /gene="dnaX"
                     /locus_tag="OQM83_000872"
     CDS             28046..29971
                     /gene="dnaX"
                     /locus_tag="OQM83_000872"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCS1519197.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    29304..29368
                     /gene="dnaX"
                     /locus_tag="OQM83_000872"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            30024..30353
                     /locus_tag="OQM83_000873"
     CDS             30024..30353
                     /locus_tag="OQM83_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCS1519198.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            30353..30958
                     /gene="recR"
                     /locus_tag="OQM83_000874"
     CDS             30353..30958
                     /gene="recR"
                     /locus_tag="OQM83_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCS1519199.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            31068..32942
                     /gene="htpG"
                     /locus_tag="OQM83_000875"
     CDS             31068..32942
                     /gene="htpG"
                     /locus_tag="OQM83_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCS1519200.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            33123..33767
                     /gene="adk"
                     /locus_tag="OQM83_000876"
     CDS             33123..33767
                     /gene="adk"
                     /locus_tag="OQM83_000876"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCS1519201.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            33899..34861
                     /gene="hemH"
                     /locus_tag="OQM83_000877"
     CDS             33899..34861
                     /gene="hemH"
                     /locus_tag="OQM83_000877"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCS1519202.1"
                     /translation="MRQTKIGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(34858..35817)
                     /gene="aes"
                     /locus_tag="OQM83_000878"
     CDS             complement(34858..35817)
                     /gene="aes"
                     /locus_tag="OQM83_000878"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCS1519203.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            35969..37273
                     /gene="gsk"
                     /locus_tag="OQM83_000879"
     CDS             35969..37273
                     /gene="gsk"
                     /locus_tag="OQM83_000879"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCS1519204.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(37406..39082)
                     /locus_tag="OQM83_000880"
     CDS             complement(37406..39082)
                     /locus_tag="OQM83_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCS1519205.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
BASE COUNT         9528 a        10049 c        10416 g         9414 t
ORIGIN      
        1 aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg
       61 acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
      121 tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc
      181 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac
      241 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
      301 aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta
      361 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
      421 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
      481 ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc
      541 gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg
      601 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg
      661 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
      721 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
      781 caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct
      841 gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
      901 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
      961 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
     1021 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
     1081 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg
     1141 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
     1201 aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca
     1261 ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
     1321 tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt
     1381 tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
     1441 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
     1501 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
     1561 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
     1621 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
     1681 cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa
     1741 tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata
     1801 tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt
     1861 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
     1921 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
     1981 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
     2041 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca
     2101 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
     2161 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg
     2221 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
     2281 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
     2341 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
     2401 acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa
     2461 tcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
     2521 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
     2581 atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
     2641 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
     2701 tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg
     2761 cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat
     2821 ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag
     2881 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
     2941 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
     3001 ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac
     3061 cagaaagcgt aattgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc
     3121 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
     3181 ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg
     3241 atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt
     3301 cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg
     3361 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
     3421 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
     3481 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
     3541 ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
     3601 ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat
     3661 acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct
     3721 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
     3781 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
     3841 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca
     3901 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc
     3961 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
     4021 gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc
     4081 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
     4141 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
     4201 cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt
     4261 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
     4321 aattctccgt ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca
     4381 gcaggattgc accctctctt tgcaggcact ggctgaagcc gttaatctga caaccacccc
     4441 ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct
     4501 gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca
     4561 tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct
     4621 ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat
     4681 gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt
     4741 cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat
     4801 atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc
     4861 cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg
     4921 caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt
     4981 actaccgggc tgatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat
     5041 ctcctgcgtt atgtctggcg cgtattgctg tttggcgcgt cttaccaact ggctgttgaa
     5101 ctgcgtgaag attattaccg tcagctaagc cggcagcacc cggagtttta cctgcgtcat
     5161 cgcacgggtg atctcatggc tcgtgcgacc aatgacgtcg attgcgtcgt gtttgccgcc
     5221 ggagaagggg tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg
     5281 atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg
     5341 gcgatcatga ttaagcgcaa cggcgatgct ttgcatgaac gctttaagct ggcacaggcg
     5401 gcgttttcca gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa
     5461 gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc
     5521 aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg
     5581 attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt
     5641 ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg
     5701 ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt
     5761 cgtgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt gccggaaggg
     5821 cgtggcgaac ttgatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg
     5881 ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact
     5941 ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt tagcgagggg
     6001 gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc
     6061 ctggcggtgg tcagccagac gccattcctt ttttctgaca ccgtggcaaa taacatcgcg
     6121 ctgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta
     6181 catgacgata ttttgcgttt accgcaaggt tacgatacag aggtgggcga gcgcggcgtg
     6241 atgctttccg gcgggcaaaa acagcgtatc tctattgctc gtgcgttatt agtcaacgcg
     6301 gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc
     6361 ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt
     6421 tcggcactga cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt
     6481 ggcaatcatg atgtgctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa
     6541 caactggagg cggcgctcga cgacgctccg gaaaatcacg aggaggccgt cgatgcgtag
     6601 ttttagccaa ctgtggccga ctctcaagcg cctgttagcg tacggttcac cgtggcgtaa
     6661 accgctgggg attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc
     6721 gctgcttatc agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt
     6781 ggttgcaggg ctggctgcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta
     6841 cgcgcagtcg ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga
     6901 cgtgatggat gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca
     6961 ggtgatttcc cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt
     7021 ggcaactgtc ctgcgcagtg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct
     7081 cgactggcga atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt
     7141 gatatatcaa cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat
     7201 caacgacggc tttaacgaaa tcatcaatgg catgagcgtc atccagcagt tccgtcagca
     7261 ggcgcgattt ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca
     7321 aaccctgcgc ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat
     7381 tctttgtgga ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt
     7441 gctgtatgcc tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac
     7501 gcaacaggcg atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga
     7561 cggaccgcgc cagcaatatg gcaatgatga ttacccgtta cagagtggca ccatcgaagt
     7621 cgataacgtg tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc
     7681 tgtgccttcg agtaactttg tggcgctggt cgggcatacc ggcagtggta aaagtaccct
     7741 cgccagttta ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg
     7801 tccattaagt tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga
     7861 tccggtggtg ctggcggata ccttcctcgc caacgtgacg ctggggcggg atatctccga
     7921 agaacgcgtc tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gtagcatgag
     7981 cgacggtatt tacacgccgc tgggcgagca ggggaataat ctctcagttg ggcaaaagca
     8041 actgctggca ctggcgcgcg tgctggtcga gacgccgcaa atcctgatcc ttgatgaggc
     8101 aaccgccagc attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg
     8161 tgaacatacc acgctggtgg tgattgctca ccgcttatcg accattgttg atgccgacac
     8221 cattctggtg cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc
     8281 tgcccaggga cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc
     8341 cagcgtgcgt gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc
     8401 aaaaatgtaa cgcactgtgc actgtcatag tgcgttttca ttttcaaact tcttaacttc
     8461 ctgctctctt tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc
     8521 agaaccatta ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa
     8581 accattcaag ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac
     8641 cgtcaccgaa gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc
     8701 ggaatacagc gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca
     8761 actcgatgaa gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg
     8821 caaaatcttc gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga
     8881 agcggcgctg taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata
     8941 aaaactgggc ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg
     9001 gccgataaag ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact
     9061 attccgggga ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg
     9121 ctgacgcagg tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac
     9181 tcgctggcgt ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa
     9241 aacatcgaac tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag
     9301 ggctcgtttg cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc
     9361 ttctcagctg tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg
     9421 catatggtgt ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc
     9481 acggtggtgc atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa
     9541 cgcgtgggct tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt
     9601 actgccattc tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat
     9661 gaaatcgcgg cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc
     9721 tggatcttcg gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc
     9781 gcgattgccg gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg
     9841 ttgattatcg gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc
     9901 ttgctgcggg tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc
     9961 ggctgtatca tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa
    10021 ggtgtgacga tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc
    10081 tggtccggtg tggtggcatt tattggctac aaactggcgg atctgacggt tggtctgcgt
    10141 gtgccggaag aacaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat
    10201 aacgcgtaac aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta
    10261 attgtgatta cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgagt
    10321 ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata
    10381 cagcagccat tcattcaaat taaacgggcg atgaaaccac atggaatggt caatggtggc
    10441 aatctgaatc cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa
    10501 gttaagatca gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct
    10561 accatttgcg cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt
    10621 atgaaactcc accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac
    10681 tggcggcagc aggtgcgcca gcgattgggc gatttgtgtt tccgaaggca ggccatcagg
    10741 cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc gcttcaggtg cctggaaaga
    10801 ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa
    10861 gctgttaccg tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg
    10921 gcgaagaaag tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc
    10981 agcatacaag gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc
    11041 actctggccg cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt
    11101 ttttagcgcc tgactcatat aactctccag taactaagct gccgcagcaa gccaaagtga
    11161 gttgagtata acgcaaattt gccactggtc cgatgggtgc aatagtctga attacgggct
    11221 aattacaggc agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc
    11281 actcttccgg cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa
    11341 actcgtgcac atggccagtt gtttagcggt tgcgattgcg ttggcggctt gcgcagataa
    11401 aagcgcggat attcagacgc cagcaccggc ggcaaatacg tctatttcag caacacaaca
    11461 acctgctatc cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact
    11521 gccgcctgat gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc
    11581 aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt
    11641 tctgcctttt aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat
    11701 taccgtgaat gacaaactgg tatttattac cgataccgtt cagccggtga tcaacaaggg
    11761 cggaactaaa gccgacctga cattggtgcc gatgcagcaa accgccgtgc cggttcaggc
    11821 cagcggtggt gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc
    11881 ggcagttcca gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt
    11941 agtagttcca gcgataacgc tgcaagtcga tttgcccgct tcccgatacc atcacacctt
    12001 ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt
    12061 ggcgattaac cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac
    12121 cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg
    12181 tgacatagcc ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt
    12241 ctttttccat cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt
    12301 gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc
    12361 agcagtgata atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt
    12421 ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg
    12481 acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg
    12541 tcaaaaaatc ccaacatagc taaactttaa ccacaactga cgtcgcaaga attgtctggc
    12601 tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt
    12661 tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa
    12721 agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc gatgtaccgg atggcaaagt
    12781 gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat
    12841 tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg
    12901 gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt
    12961 cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa
    13021 aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta
    13081 gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt
    13141 ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg
    13201 cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac
    13261 atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat
    13321 ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa
    13381 ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgattga ttgatcattt
    13441 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    13501 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa
    13561 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    13621 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    13681 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    13741 catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    13801 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    13861 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg
    13921 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    13981 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    14041 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    14101 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    14161 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    14221 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    14281 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    14341 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    14401 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    14461 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    14521 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    14581 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    14641 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca
    14701 agcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg
    14761 agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt
    14821 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    14881 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    14941 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    15001 aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg
    15061 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg
    15121 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
    15181 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    15241 aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc
    15301 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
    15361 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    15421 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    15481 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    15541 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
    15601 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata
    15661 gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc
    15721 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    15781 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    15841 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    15901 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    15961 gatcacatag ggtatgtctt catggtgaaa agtataaaat tcttaataaa cagccggtta
    16021 tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg
    16081 tcgtatagtt tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc
    16141 gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca
    16201 attgtctggc aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac
    16261 atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt
    16321 cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg
    16381 catattaata ccataactac tgaacaacat aaaggtgtca tccagatatt cgtcgatctg
    16441 ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtctcaat
    16501 atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc
    16561 atgattgctt tcttcaaggt ttgcaaggca gtcatgatac agggtttcac agagaaactt
    16621 aagctgtgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag
    16681 cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc
    16741 tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt attacgaaaa
    16801 attacaatta ggaaggaatc gggacaggac tcactttttc atactagcac taacccgcag
    16861 caggtaaaag cagtctgctc gaactgttca taacatttca ggattatcgc cagatgaaat
    16921 tggtgacact aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag
    16981 aattaccgca aataattaag aatagcctct aaatgattat ggattattaa atctattagc
    17041 gctacttaat ataaattaac taacaatcag tattttttat gaattttatc cgtggttaat
    17101 actggttttc gtatgagatc ctgagttggt ggttcaatta ctccttaatg ttcgtaggtt
    17161 atgcataaaa aaggccgctt gcgcggcctt agtgattaca cgttgtatca atgatgatcg
    17221 acagtatggc tgtgctcgat atcttcattc ttgcggctaa agcggcggcg aaccaccaca
    17281 aagaataccg gaacgaagaa gattgccagt accgttgcgg tcaccatccc gcccattaca
    17341 ccggtaccta ctgcgttctg cgcgccggaa ccagcaccag tactgataac cagcggcata
    17401 acgccgagga taaacgccag cgaggtcatc aggatcggac gtaaacgcat ccgcactgca
    17461 tcaagcgtcg cttcaatcag acctttacct tctttatcca tcaagtcttt ggcgaattcg
    17521 acgataagta tcgcgttctt cgccgacaac ccaatggttg tgagcaggcc tacctggaag
    17581 taaacgtcat tggtcaggcc acggaaggtg gcagccagca acgcaccgat aacccccagc
    17641 ggaacgacca gcataacgga gaacggaatc gaccagctct cgtacagcgc cgccagacac
    17701 aggaatacga caatcaacga aatcgcgtac agtgaaggtg cctggttgcc ggagagacgt
    17761 tcctgatagg acatccccgt ccagtcatag ccaacaccgg taggcagttt gctcgccagt
    17821 tgttccatca gctccattgc ttcaccggta cttttacccg gtgccgcctg gcctaagatt
    17881 tccatggatg gcaggccgtt gtaacgttcc agacgcggcg aaccgtactc ccaacgagaa
    17941 gaggagaacg ccgagaatgg caccatctga ccatcagcag cacgaacata ccagtcgccg
    18001 atatcatccg gcagcatacg gtatttcgct tctgacatga cgtaaacttt cttcacacga
    18061 ccgcggtcga taaagtcgtt cacatagcta ccgccccatg cagcgcccag agaggtgtta
    18121 atgtcgttga tagaaacacc cagcgcctgc gctttttcct ggtcgatatc aatcttaaac
    18181 tgcggggtat cttccagacc gtttggacgt acgctggtca gcatatcagg gtgcttcgct
    18241 gcttctgcaa gcagctggtt acgcgcctga gtcagttttt cgtgaccaag gccagcctgg
    18301 tcaatcagct caaagtcaaa gccggttgca gtacccagtt ccacgattgc gggcaggtta
    18361 aaggcgaaaa ccatcgcatc tttgatttgc gagaaagcgc gtgttgcacg catggtaatc
    18421 gcttcaactt tgttttcttc gcccggacga tcggcccagt ccttcaagga aacgaacgca
    18481 ataccggtat tctgaccacg tcccgcaaag ccgaagccgt taacggcgaa caccgactca
    18541 acgttgttct tttctttggt cagatagtaa tgcgttacct cattgagcac tttctgcgta
    18601 cgttcctgcg ttgcacctgc tggcagctga accatggtca taaatacgcc ctggtcctca
    18661 tctggcaaga aggagcttgg cagacgcacg aacagatagg ccatgccgac cacgatgatc
    18721 agatacagca ccaggtaacg ccccgtactg cgcagaatac cgcctacgct gtcggtgtag
    18781 tggtgcgtgc tcttctcgaa catgcggtta aaccagccga agaagccttt tttaccttcc
    18841 ccgtgatcgc ctttggcaat cggtttcagc atggtggcac aaagagccgg agtcaggatc
    18901 aacgctacca gtaccgacag cgccattgct gaaacaatgg taatagagaa ctgacgatag
    18961 atagcaccag tagaaccgcc aaagaaggcc atcggtacga ataccgccga cagtaccatc
    19021 gcgataccga ccagagcgcc ctgaatctgc cccatcgact tacgggtagc ttcttttggc
    19081 ggcaaacctt cttccgccat aacacgctcc acgttttcta ccacaacgat agcgtcatcc
    19141 accaacaagc cgatggcgag caccatcccg aacattgtta gcgtgtttat cgagaagcca
    19201 aaggcggcaa ggacggcaaa ggtcccgagc aataccaccg gtacggcaat ggtcggaatc
    19261 aacgtcgcgc ggaagttctg caggaacaga tacataacca ggaacacgag gatgatcgct
    19321 tcgaccagcg ttttaaccac ttcgtgaata gagattttca cgaacggcgt ggtgtcgtac
    19381 gggtaaacaa ttttcagacc cgacgggaag aacggttcca tcttcgccag ttcagcacgg
    19441 attgccgcag cggtatccag cgcgtttgca ccggtcgcca gcttgatccc cagaccggaa
    19501 gccggttggc cgttaaactc tgcgatgatg tcgtagttct caccacccag ctcaatcttc
    19561 gccacatcac gcagcagtac gcgggaacca tcctgattca ctttcagcag gattttgccg
    19621 aactcttcag tagaggtcag acgcgtctga gcaataatag aggcgttaag ctgttggcct
    19681 ttcaccggcg gcgtaccacc gagctgaccc gccgcaacct gggcgttctg cgctttgatg
    19741 gcggtaatga catcaaccgg cgttagctgg aatttgttca gctcattcgg gttcatccag
    19801 atacgcatcg cgtactgtga accgaacaac tgaacatcac ccacgcccga cgtacggctg
    19861 atggcatctt tcatattcgc cgctacgtag tcagagatat cctcctgcgt catggtgcca
    19921 tcggtgttga taacgccgac aaccatcagg aagctgctgg atgatttctc aacgctcacc
    19981 ccttgctgct gaacttcttg cggcagcaac ggcatcgcca gctgcagttt gttctgcacc
    20041 tgaacctgcg cgatatccgc atcagtacca gactcaaagg tcagggtgat ctgcacggta
    20101 cccgtggagt cactgttaga ggacatgtac atcaggttat cgataccgtt catattctgt
    20161 tcgataacct gtgtcaccgt gtcctgcact gttttcgcat cagcgccggg gtaggaggcg
    20221 gagatcgtta ctgccggcgg tgcaatcgta ggatattgcg ccaccggcag tttgaggatc
    20281 gccagccccc ctgccaacat gatgataatg gcgatcaccc acgcaaaaat cgggcgatcg
    20341 ataaagaaat taggcatgtc ttaacggctc ctgtttaagt taagacttgg actgttcagg
    20401 ctgagcaccg cttgcggctt gctggttatt atcagcggta acttcttgtg cttttacctg
    20461 gacaccagga cgcactttct gcagcccact tattactacg cgatcgcctg ctttcagacc
    20521 ttctgtcacc agccacttat cgccaatagc ctggcttgca acgatcggac gggtttccac
    20581 tttgtcatcc gcgccaacta ccagtacggt ggcatcacca cgcggcgtac gggttacgcc
    20641 ctgttgcggg actaaaatag cgtttggatt aagcccttct tccagacgtg cgcgcacgaa
    20701 catacccggc agcagagtgt gatccgggtt cgggaagata gcgcgtaggg tgatagaccc
    20761 agtggtctga tcaacggtaa cgtcagagaa ttccagcgta ccgtcctgcg ggaacttaat
    20821 gccgtcactg gtgatcagcg acactttggc tttgccgttc tcttgtttca gcgtgccatt
    20881 cgccagttcc tgtttcaggc gcaggaagtc gttgctggac tgggtcacat caaggtagat
    20941 cggatcaagt tgctgcacgg ttgccagcgc agtcgcctga ccgttctgta ccaatgcgcc
    21001 ttccgtcacg ttcgacttac caatgcgacc gctaatcgga gaggtgactt tggtgtaagc
    21061 cagattgatc cgcgcagttt caacggcagc tttcgccgca gttaccgcag cattcgcctg
    21121 ttgcgcatca gccagagcct gatcgtactc ttgcttactg atgtactgag taccgagcag
    21181 tttctgataa cgattcaccg tcaattgcgc gatattggct gcagcctggg ctttcgccag
    21241 atcacctttc gcactgtcgt atgtcgcctg ataggtcgca ggatcaatct gatagagaga
    21301 gacaccagct tcgatgtcgc taccttcttt gaaattacgc ttcaggataa tcccgctaac
    21361 ttgaggacga acttctgcga tccggtaggc actggtgcga cccggaagct cggttgtgat
    21421 ctgcagaggt tcagttttga ctgttactac gccaacggcg ggcatctgct ggccaccttg
    21481 ttgggcctgt ttgtcgtcac atcctgttag ggctaagctg cctgagagca tcagaacgac
    21541 cgccagaggc gtaaaccctc tgtttttgtt catatgtaaa cctcgagtgt ccgatttcaa
    21601 attggtcaat ggtcaaaagt taataaaccc attgctgcgt ttatattatc gtcgtgctat
    21661 ggtacataca ttcacaaatg tatgtaaatc taacgcctgt aaattcacga acatatggca
    21721 cgaaaaacca aacaagaagc gcaagaaacg cgccaacaca tcctcgatgt ggctctacgt
    21781 cttttctcac agcagggggt atcatccacc tcgctgggcg agattgcaaa agcagctggc
    21841 gttacgcgcg gtgcaatcta ctggcatttt aaagacaagt cggatttgtt cagtgagatc
    21901 tgggaactgt cagaatccaa tattggtgaa ctagagcttg agtatcaggc aaaattccct
    21961 ggcgatccac tctcagtatt aagagagata ttaattcatg ttcttgaatc cacggtgaca
    22021 gaagaacgac gtcgattatt gatggagatt atattccaca aatgcgaatt tgtcggagaa
    22081 atggctgttg tgcaacaggc acaacgtaat ctctgtctgg aaagttatga ccgtatagaa
    22141 caaacgttaa aacattgtat tgaagcgaaa atgttgcctg cggatttaat gacgcgtcgg
    22201 gcagcaatta ttatgcgcgg ctatatttcc ggcctgatgg aaaactggct ctttgccccg
    22261 caatcttttg atcttaaaaa agaagcccgc gattacgtcg ccatcttact ggagatgtat
    22321 ctcctgtgcc ccacgcttcg taatcctgcc actaacgaat aaccctgaat ctgactccag
    22381 gatttttcct ggacattttc gtcgttgcta ttctggttca ctgcgtcgtg atattcttgc
    22441 ggtttgactt tttcaggtcg ttcttcaggt tcagaaacct tcattcatca tgactatgtt
    22501 ccagtattac aaacgatcac ggcattttgt tttttcagca tttattgctt ttgtttttgt
    22561 cttgttatgc cagaacacgg cgtttgcgcg ggcgtcatcg aatggtgatc tgccgacaaa
    22621 agcggacctg caggcgcaac ttgactcact aaataaacaa aaagatcttt ctgctcagga
    22681 caaactggtg cagcaggatc tgacagatac attagccacc ctcgataaaa tcgatcgcat
    22741 aaaagaagag acagttcagc tacggcaaaa agtcgctgaa gcgccggaaa aaatgcgcca
    22801 ggcgaccgcg gcgttaacag cacttagcga tgtcgataac gacgaagaaa cgcgcaaaat
    22861 tctgagtacg ctttcgttgc gccagctgga aactcgcgtt gcccaggcgc tggacgattt
    22921 gcaaaacgca caaaacgatc tggcgtctta taacagccag ctggtttcgt tacagacgca
    22981 gcccgaacgc gtgcaaaatg cgatgtataa cgcttcgcag cagctgcaac aaattcgcag
    23041 tcatctggat gggactgatg tcggcgagac tgccttacgt cccagccaga aagtgttaat
    23101 gcaggctcag caggcgttgc tgaatgcgga gattgaccag cagcgtaaaa gcctggaagg
    23161 gaacaccgtc ttgcaggata ccttgcaaaa gcaacgtgat tacgtgacgg cgaacagcgc
    23221 ccgtctggag caccagttgc agctgttgca agaagcggta aacagcaagc gcctgacttt
    23281 aactgaaaaa acggcgcagg aagccgtctc cccggatgaa gccgcgcgta ttcaggctaa
    23341 tccgctggtg aaacaggaac tggaaattaa ccagcagtta agtcagcgtc tgattaccgc
    23401 gactgaaaac ggtaatcagt tgatgcagca aaacattaaa gtcaaaaact ggctggagcg
    23461 ggcgctgcaa tcggaacgca atattaaaga gcagattgcc gtcctgaagg gcagcctgct
    23521 gttgtctcgt atcctttacc agcaacaaca aacgctgccc tcggcggatg aactggaaaa
    23581 catgaccaac cgcatcgcgg atttgcgtct cgaacagttt gaagttaacc agcagcgtga
    23641 tgcactcttc cagagcgatg cgttcgtcaa caaactggaa gaaggtcaca ccaacgaagt
    23701 caacagcgaa gttcacgatg cgttattgca agtggttgat atgcgtcgcg aattgctgga
    23761 tcaactcaac aaacagttgg gtaaccagct gatgatggcc attaacctgc aaatcaacca
    23821 gcagcagtta atgagcgtat cgaaaaacct gaaatccatc ctgactcagc aaatcttttg
    23881 ggtgaacagt aaccgtccaa tggactggga ctggatcaaa gcgttcccgc aaagcctgaa
    23941 agatgaattt aagtcgatga aaatcacggt gaactgggaa aaagcctggc ctgccgtttt
    24001 tatcgctttc ctcgccggtt tgccgctgct gttgattgcc gggctgatcc actggcgtct
    24061 gggctggctg aaagcgtatc aacagaaact ggcttccgct gtgggttccc tgcgtaacga
    24121 tagccagctc aacacaccaa aagcgatcct tatcgacctg atccgcgcgc tgccggtgtg
    24181 cctgattatt ctcgcggttg gcctgattct gttgaccatg cagctcaaca tcagcgaact
    24241 gctatggtcg ttcagtaaaa aactggcgat tttctggctg gtgtttggcc tgtgctggaa
    24301 ggtactggag aaaaatggcg ttgccgtgcg tcacttcggc atgccggagc agcagaccag
    24361 ccactggcgt cggcaaattg tccgcatcag tctcgcgttg ctgcctatcc atttctggtc
    24421 tgtggtggca gaactttccc cgctgcatct gatggatgat gtgctggggc aggcgatgat
    24481 tttcttcaac ctgctgctga ttgccttcct ggtatggcca atgtgccgcg aaagctggcg
    24541 tgataaagag tcgcacacca tgcgactggt caccattacc gtgctgtcga ttatcccgat
    24601 tgcattgatg gtgctgactg ccacgggtta tttctacacc acgctgcgcc tctccgggcg
    24661 ttggattgaa accgtttatc tggtgatcat ctggaacttg ctctaccaga cggtattgcg
    24721 cggcttaagc gtagcggcgc ggcgtatcgc ctggcgtcgt gcgctggcgc gtcggcagaa
    24781 tctggtgaaa gagggcgcag aaggtgccga accgccggaa gaacccacca ttgcactgga
    24841 acaggttaac cagcagacgc tgcgtattac catgttgctg atgtttgcgc tgttcggtgt
    24901 catgttctgg gcaatttggt ccgatttgat caccgtgttc agctatctcg acagcatcac
    24961 gctctggcat tacaacggca ctgaagctgg cgctgcggtg gtgaaaaacg tcaccatggg
    25021 cagtctgttg tttgcgatta tcgcctcaat ggtggcctgg gcgttgattc gcaacctgcc
    25081 tggtttactg gaagtgctgg tgctctcgcg actgaatatg cgtcagggcg cgtcgtatgc
    25141 cattactacc atccttaact acatcattat tgccgttggt gcgatgacgg tgttcggatc
    25201 gctgggcatc tcttgggata aactccagtg gctggccgca gcattatccg taggtcttgg
    25261 ttttggttta caagaaattt tcggtaactt cgtctccggt ttgatcattc tgttcgaacg
    25321 tccggtgcgt attggcgata cggtaaccat tggtagcttc tcggggacgg taagtaagat
    25381 ccgtattcgt gcgacaacga ttaccgattt cgatcgcaaa gaagtgatca tcccgaacaa
    25441 agcgtttgtt accgagcgtc tgatcaactg gtcgttgact gacactacta cgcgtctggt
    25501 gatccgtctc ggcgtggcct atggctccga tctggaaaaa gtgcgtaaag tgttactgaa
    25561 ggcggcgact gagcacccaa gggtgatgca cgaaccaatg ccggaagtct tctttacggc
    25621 gtttggtgcc agcacgttgg atcatgagct gcgtctgtat gtgcgtgaac tgcgtgaccg
    25681 tagtcgtact gtcgatgagc tgaaccgtac tatcgatcag ctgtgccgtg aaaacgacat
    25741 caacattgcc tttaaccagc ttgaagtgca tctgcacaac gagaagggcg atgaggtgac
    25801 ggaagtcaaa cgcgactaca aaggcgatga cccgacgcca gcggtagggt aataaccaaa
    25861 gggggcgaca tttagttgcc ccgagattac tgacaaagtg cgcgttgttc atgccggatg
    25921 cggcgtgacc gccttatccg gcctacgaaa ccgcaagaat tcaatgtatt gcaggagcgg
    25981 tgtaggcctg ataagcgtag cgcatcaggc agttttgcgt ttgcccacaa tcttagggga
    26041 catttagcgg ccccatttat ttctcacttt tccgccttat catcgcgcgt taatttcttt
    26101 tcataatcgc gttttacaat atccagcgcg cgcagcacgg tgcgggctgg gatctgattt
    26161 tcttccagca gcacaatcaa atcgacagcc agtttgacat cgtcaggggc attttcaagt
    26221 gacatactct ctccattgct agcgggttaa gcgcgctagc ctgttttcga ttttttccag
    26281 cgcatggcgg cagcgtgcca ggcgcgcttc ataggcttcc acttcacgat gcagcgtttg
    26341 ctgttccacg agatcggtca cccgcgccag gcgggctctg cgttcggcaa ccatctcacg
    26401 tagccgccgc tcaaaatcct gatgctgaat acgtttacgc tgccagcggg caattttcgg
    26461 tggcgcactg tcccactcgc gcaatgacca ggcggaggct tcacgcgcga tggcttccag
    26521 ttgtgccgcc agatgttccg ccagccaggc cacttgcggc agctgttgct gctcaaccgc
    26581 atgacaaagc gcagccagat tatcgcccgc ctcgtcgaga caagcctgta gtgttgtcgc
    26641 acgagtctga aaaagatgcc tgtcgaaacg agcacttagc gtggcgaact gtgacaccgg
    26701 ggcacaacgc tgacgcagcg tagcgagctg tccttccagt ttttccagca gcagggcggt
    26761 tttcacgata agacctcaat gaaaatgata attgttatgc taaagtagcc actctgtaag
    26821 ctgacaacac tcaatatgca acgaatcatt ttaatcatca ttggctggct ggcggtagtg
    26881 ctgggtacgc tgggcgtggt attaccggta ttaccgacga cgccgtttat cctgctggcg
    26941 gcctggtgct ttgcccgttc ttccccgcgc tttcacgcct ggttgctgta ccgctcatgg
    27001 tttggcagct atctacgttt ctggcagaaa catcatgcga tgccgcgcgg cgtcaaaccg
    27061 cgggcgattt tgcttatttt gctcacgttt gccatttctc tgtggttcgt ccagatgcca
    27121 tgggtgcgca tcatgttgct ggtaattctc gcctgtttgc ttttctatat gtggcgaatt
    27181 ccggtgattg atgaaaagca agaaaagcac tgaagcacaa caatcgcagt tgcaattatt
    27241 gcgtacagcc agtacattct ggcgttttcg agcacaggcg caggcggtca aaggttaaac
    27301 aactgttact tttgatacgt ttaaaacgcg ccgtgagtac caccgtaaca agcaggcata
    27361 cacttatgac cgcgactgca cagcagcttg agtatctcaa aaatagcatc aaaagcattc
    27421 aggactaccc aaaacccggc attcttttcc gcgatgtcac cagcttactg gaagacccga
    27481 aagcttacgc tctcagcatc gacttgctgg ttgagcgtta caaaaatgcg ggcattacca
    27541 aagttgtcgg caccgaagcg cgtggcttct tgtttggcgc tccggtagct ctgggtctgg
    27601 gcgttggctt tgtaccggtc cgtaaaccgg gcaaactgcc gcgtgaaacc atcagtgaaa
    27661 cttacgacct ggaatacggc accgatcagc tggagatcca cgttgatgcc atcaaaccgg
    27721 gcgacaaagt tctggtggtg gacgatctgc tggcaaccgg cggcactatc gaagcgaccg
    27781 ttaaactgat ccgtcgtctg ggtggtgaag tggctgacgc tgcgttcatt atcaacctgt
    27841 tcgatctcgg cggcgaacag cgtctcgaaa aacagggcat taccagctac agccttgtcc
    27901 cgttcccggg ccattaatta tcgccagtct tgtgctgccc acgctacgga cagcacaaga
    27961 tgtgcattca gcctcgccgt tctgacgggg ctgtgttagc attacccctt cgtgaatcca
    28021 ccttccagcg tttcagagcc tgccaatgag ttatcaggtc ttagcccgaa aatggcgccc
    28081 acaaaccttt gctgacgtcg tcggccagga acatgtgctg accgcactgg cgaacggctt
    28141 gtcgttaggg cgtattcatc atgcttatct tttttccggc acccgtggcg tcggaaaaac
    28201 ctctatcgcc cgactgctgg cgaaggggct aaactgcgaa accggcatta ccgcgacgcc
    28261 gtgcggcgtg tgcgataact gtcgtgaaat cgagcagggg cgctttgtcg atctgattga
    28321 aatcgacgcc gcctcgcgca ccaaagttga agatacccgc gacctgctgg ataacgtcca
    28381 gtacgctccg gcgcgtggtc gtttcaaagt ttatctgatc gacgaagtgc atatgctgtc
    28441 gcgccacagc tttaacgcac tgttaaaaac ccttgaagag ccgccggagc acgttaagtt
    28501 tctgctggcg acgaccgatc cacagaaatt gccggtgacg attttgtcgc gctgtttgca
    28561 atttcatctc aaggcgctgg atgtcgagca aattcgccat caacttgagc acatcctcaa
    28621 cgaagaacat atcgctcacg agccgcgggc gttgcaattg ctggcgcgcg ccgctgaagg
    28681 cagcctgcga gatgccttaa gtctgaccga ccaggcgatt gccagcggtg acggccaggt
    28741 ttcaacccag gcagtcagtg cgatgctggg tacgcttgat gacgatcagg cgctgtcgct
    28801 ggttgaagcg atggtcgagg ccaacggcga gcgcgtaatg gcgctgatta atgaagccgc
    28861 tgcccgtggt atcgagtggg aagcgttgct ggtggaaatg ctcggcctgt tgcatcgtat
    28921 tgcgatggta caactttcgc ctgctgcact tggcaacgac atggccgcca ttgagctgcg
    28981 gatgcgtgaa ctggcgcgca ccataccgcc gacggatatt cagctttact atcagacgct
    29041 gttgattggt cgcaaagaat taccgtatgc gccggaccgc cgcatgggcg ttgagatgac
    29101 gctgctgcgc gcgctggcgt tccatccgcg tatgccgctg cctgagccag aagtgccacg
    29161 ccagtccttt gcacccgtcg tgccaacggc agtaatgacg ccaacccagg tgccgccgca
    29221 atcagcgccg cagcaggcac cgactgtacc gctcccggaa accaccagcc aggtgctggc
    29281 ggcgcgccag cagttgcagt gcgtgcaggg agcaaccaaa gcaaaaaaga gtgaatcggc
    29341 agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg gaaagactgg cttcggtcac
    29401 cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa aaagcgtcag ccaaaaaaga
    29461 agcgtatcgc tggaaggcga ccactccggt gatgcagcaa aaagaagtgg tcgccacgcc
    29521 gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg gaactggcgg cgaagctagc
    29581 ggcagaagcc attgagcgcg acccgtgggc ggcacaggtg agccaacttt cgctaccaaa
    29641 actggtcgaa caggtggcgt taaatgcctg gaaagaggag agcgacaacg cagtatgtct
    29701 gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt gcacagcaaa aactggctga
    29761 agcgttgagc atgttaaaag gttcaacggt tgaactgact atcgttgaag atgataatcc
    29821 cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac gaagaaaaac ttgcgcaggc
    29881 gcgcgagtcc attattgcgg ataataatat tcagaccctg cgtcggtttt tcgatgcgga
    29941 gctggatgaa gaaagtatcc gccccatttg atcgtaagca cagcttacgt tcgtcatcct
    30001 taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg tctgggtaac ctgatgaagc
    30061 aagcccagca gatgcaagaa aaaatgcaga aaatgcagga agagatcgcg cagctggaag
    30121 tcaccggcga atctggcgca ggtctggtaa aagtgaccat caacggtgca cacaactgcc
    30181 gtcgcgtaga gatcgacccg agcctgctgg aagacgacaa agagatgctg gaagacctgg
    30241 tggctgcagc attcaacgac gcagcacgtc gtattgaaga aacgcagaaa gaaaaaatgg
    30301 cctctgtatc ctccggaatg cagctgccgc ctggctttaa gatgccgttc tgatgcaaac
    30361 cagcccgctg ttaacacagc ttatggaagc actgcgctgt ctgccgggcg ttggcccgaa
    30421 gtcggcgcag cgtatggcgt tcacgctgct tcagcgcgat cgtagcggcg ggatgcgttt
    30481 ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac tgcgccgatt gccgcacttt
    30541 caccgaacag gaagtctgta acatctgttc gaatccgcgt cgtcaggaaa acggtcaaat
    30601 ctgtgtggtg gagagtccgg cggacatcta cgccattgag cagacggggc agttttctgg
    30661 tcgttatttt gtgttgatgg ggcatctgtc accgctggac ggcatcggtc cggatgatat
    30721 cgggcttgat cgtctggaac agcgtctggc agaggaaaaa atcactgaag tgatcctcgc
    30781 caccaatccc acggttgaag gtgaagctac cgctaactac attgccgagc tttgcgcgca
    30841 atatgacgtg gaagccagcc gaatcgctca tggcgtaccg gttggcggcg aactggaaat
    30901 ggtcgatggc accacgctgt cacactccct tgccgggcgt cataagattc gtttttaagc
    30961 aaacgagagc aggatcacct gctctcgctt gaaattattc tcccttgtcc ccatctctcc
    31021 ctcatcctgt ttttaacctt aaaatggcat tattgaggta gacctacatg aaaggacaag
    31081 aaactcgtgg ttttcagtca gaagtgaaac agcttctgca cctgatgatc cattctctct
    31141 attccaataa agaaatcttc ctgcgtgagc ttatctctaa cgcctccgat gcggcggaca
    31201 agctgcgttt ccgtgcgcta tctaacccgg acctgtacga aggtgatggc gaactgcgcg
    31261 ttcgtgtctc tttcgataaa gacaagcgca cgctgaccat ctctgataac ggcgtgggga
    31321 tgacccgcga cgaagtgatt gaccatctgg ggactatcgc taaatccggt accaaatcat
    31381 tcctcgaatc cctgggttct gaccaggcga aagacagcca gctgatcggt cagtttggtg
    31441 ttggtttcta ctctgcgttt atcgtggccg acaaagtgac cgtgcgtact cgcgcggcag
    31501 gcgaaaaacc agaaaacggc gtcttctggg aatcggctgg cgaaggtgaa tataccgttg
    31561 ccgacatcac caaagaagat cgtggtactg aaatcaccct gcatctgcgt gaaggcgaag
    31621 acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag caaatactcc gaccatatcg
    31681 cgctgccggt agagatcgaa aaacgcgaag agaaagacgg cgaaaccgtt atctcctggg
    31741 agaaaatcaa caaagcgcag gcgctgtgga ctcgtaacaa gtcggaaatc accgatgaag
    31801 agtacaaaga gttctacaaa cacatcgccc acgactttaa tgatccgctg acctggagcc
    31861 acaaccgcgt tgaaggtaag caggagtaca ccagcctgct gtacattccg tcccaggctc
    31921 cgtgggatat gtggaaccgt gatcataaac acggcctgaa actgtacgtc cagcgtgtgt
    31981 tcatcatgga cgacgcagaa cagttcatgc cgaactatct gcgcttcgtg cgtggtctga
    32041 ttgactccag cgatctgccg ctgaacgttt cccgtgaaat cctccaggac agcacggtaa
    32101 cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca aatgctggaa aaactggcga
    32161 aagacgacgc ggaaaaatac cagaccttct ggcaacagtt tggcctggta ctgaaagaag
    32221 gtccggcgga agatttcgct aaccaggaag cgatcgccaa actgctgcgt tttgcttcta
    32281 cccataccga ttcttctgcg cagaccgtat ctctggaaga ctacgtttcc cgcatgaaag
    32341 aagggcagga gaaaatctac tacatcaccg cagacagcta tgcggcagcg aagagcagcc
    32401 cgcacctgga actgctgcgt aagaaaggca tcgaagttct gctgctttcc gaccgcatcg
    32461 atgagtggat gatgaactat ctgactgagt tcgacggtaa accgttccag tcggtgtcta
    32521 aagttgacga gtcgctggaa aaactggctg acgaagttga tgagagcgcg aaagaagcgg
    32581 agaaagcact gactccgttc atcgaccgtg tgaaagccct gctcggcgag cgcgtgaaag
    32641 atgtccgtct gactcaccgt ctgaccgata cgccagcgat cgtttcgacc gacgcggacg
    32701 aaatgagcac tcagatggcg aaactgttcg ctgcggcggg ccagaaagtg ccagaagtga
    32761 aatacatctt cgaactgaac ccggatcacg tactggtgaa acgtgcggca gatactgaag
    32821 atgaagcgaa gttcagcgag tgggtagaac tgctgctgga tcaggcgctg ctggcagaac
    32881 gcggcacgct ggaagatccg aacctgttta ttcgtcgtat gaaccagctg ctggtttcct
    32941 gatgtcatgc cggatgacct ttgtgtcatc cggcattttt cttttcatca tctgcacttt
    33001 ccgcaaatta tctcgccatt aaccgtttca gcctcaggtg cctttcttga ggcaatcgcc
    33061 tgttggtggt atcgtttatc gctttttcaa aaaaatcgac acattttaag gggattttcg
    33121 caatgcgtat cattctgctt ggcgctccgg gcgcggggaa agggactcag gctcagttca
    33181 tcatggagaa atatggtatt ccgcaaatct ccactggcga tatgctgcgt gctgcggtca
    33241 aatctggctc cgagctgggt aaacaagcaa aagacattat ggatgctggc aaactggtca
    33301 ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc tcaggaagac tgccgtaatg
    33361 gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc agacgcgatg aaagaagcgg
    33421 gcatcaatgt tgattacgtt ctggaattcg acgtaccgga cgaactgatt gttgatcgta
    33481 tcgtaggccg ccgcgttcat gcgccgtctg gtcgtgttta tcacgttaaa ttcaatccgc
    33541 cgaaagtaga aggcaaagac gacgttaccg gtgaagaact gactacccgt aaagacgatc
    33601 aggaagaaac cgtgcgtaaa cgtctggttg aataccatca gatgactgca ccgctgatcg
    33661 gctactactt caaagaagcg gaagcgggta acaccaaata cgcgaaagtt gacggcacca
    33721 agtcggttgc tgaagttcgc gctgatctgg aaaaaatcct cggctaatca agccgcagca
    33781 gatctgcttt cgggcaggtc tgctcatcca cctcaggcaa tacctctcac agcaattagt
    33841 tcttcttcct cacttttccg ctacaattat caacaagttg aatcgataag aggcggtaat
    33901 gcgtcagact aaaatcggta tcctgctggc aaacctgggt acgcccgatg cccccacacc
    33961 tgaagcggta aaacgctatc tgaaacaatt tttaagcgac agacgcgtgg ttgatacctc
    34021 acggttgtta tggtggccat tgctgcgcgg cgttattttg ccgctgcgct cgccgcgtgt
    34081 ggcgaagctg tacgcctcgg tctggatgga aggtggctcg ccgctgatgg tttacagccg
    34141 ccagcaacag caggcgctgg cacaacgttt accggagacg cccgtagcgc tgggaatgag
    34201 ctacggctcg ccatcactgg aaagcgccgt cgatgaactc ctggcagagc atgtagatca
    34261 tattgtggtg ctgccgcttt atccgcaatt ctcctgttct acggtcggtg cggtatggga
    34321 tgaactggca cgcattctgg cgcgcaaacg tagcattccg gggatatcgt ttattcgtga
    34381 ttacgccgat aaccacgatt acattaatgc actggcgaac agcgtacgcg cttcttttgc
    34441 caaacatggc gaaccggatc tgctgctgct ctcttatcat ggcattcccc agcgttatgc
    34501 agatgaaggc gatgattacc cgcaacgttg ccgcacaacg actcgcgaac tggcttccgc
    34561 actggggatg gcaccggaaa aagtgatgat gacctttcag tcgcgctttg gccgggaacc
    34621 ctggctgatg ccttataccg acgaaacgct gaaaatgctc ggagaaaaag gcgtaggtca
    34681 tatacaggtg atgtgcccgg gctttgctgc ggattgtctg gagacgctgg aagagattgc
    34741 cgagcaaaac cgtgaggtct tcctcggtgc gggcgggaaa aaatatgaat atattccagc
    34801 gcttaatgcc acgccggaac atatcgaaat gatggctaat cttgttgccg cgtatcgcta
    34861 aagctgagcg gtaaagaact gagcgccgtc gcgaagagcc tcgtcggcgg ttttcatcat
    34921 ccgtgaataa tgcaaaaagg catgcagcgt acctgggtag agtttgaact cacagggctg
    34981 ctgatgcgcc gctaacgtct ggtaaagcag acggctgtca tccagcagcg gatcgaactc
    35041 cgccccggca ataaaacagg gcggaacttc gcgagtgaga tcattattaa acagacagta
    35101 atacggtgac tcgcggtccg cgtcgttgct taaatacgcc tcttcataca tctgcaaatc
    35161 ctgttgcgtt aagccatccc agacaccgcc caacagacga cgagtcacgg aatcccgtaa
    35221 tccgtaaagc ccataccaca gcaaaacacc cgcaatttta ccgcaatcga tctgtttatc
    35281 acgcaaccac aacgcactgg cgagcgccag catggcacct gcggaatcac cggcaaagcc
    35341 aatgcgggac atattgattt gataatcctc cgcctgctgg tggaaataac aacaagcagc
    35401 cacaatttcc tctatcgctt gcggaaaacg cgcttcaggt gaaagggtgt aatcaatacc
    35461 aatcaccgta cattggctgt agcttgccag caggcgcatg atgcgatcgt gggtatcgag
    35521 attgccgaga ataaaaccgc ctccatgcaa ataaaatagc gtcgccgggc tatctggctg
    35581 cggacaaaag agacgtgttt ccacctgccc atattttgtt ggaaccatgt aagctctggt
    35641 tgccatttct ggagcgcccg cattccagaa tcggcgctca agcgtgtaat actgtcgttg
    35701 ctcagcaatc gttcccgttg cgggccagga cggtaaatcc ggctgaagag tattcacaac
    35761 ggtcttcatt tcagcagaaa taaggtccag aacaggtagt ttgttttccg gcttcataca
    35821 aaaactcctt tcaaattacg tcattgtaag gaaccactgc catgaaaatg cgatcccgcc
    35881 tgctgatatt gaaactggct gcgtctcgcg cgctcccgtc agattgtgtt aacattcgcc
    35941 gctcagttaa ccacccgtaa aaacaaccat gaaatttccc ggtaaacgta aatccaaaca
    36001 ttacttccct gtaaatgcac gcgatccgct gcttcagcaa ttccagccag aaaacgaaac
    36061 cagcgccgcc tgggtcgtag gtattgacca gacgctggtc gatattgaag cgaaagtgga
    36121 tgatgaattc attgagcgtt atggattaag cgccgggcat tcactggtga ttgaggatga
    36181 cgtagccgaa gcgctttatc aggagctaaa acagaaaaac ctgattaccc atcagtttgc
    36241 gggtggcacc attggtaaca ccatgcacaa ctactcggtg ctcgcggacg accgttcggt
    36301 gctgctgggc gtcatgtgca gcaatattga aattggcagt tatgcctatc gttacctgtg
    36361 taacacttcc agccgtaccg atcttaacta tctacaaggc gtggatggcc cgattggtcg
    36421 ttgctttacg ctgattggcg agtccgggga acgtaccttt gctatcagtc caggccacat
    36481 gaaccagctg cgggctgaaa gcattccgga agatgtgatt gccggagcct cggcactggt
    36541 tctcacctct tatctggtgc gttgcaaacc gggtgaaccc atgccggaag caaccatgaa
    36601 agccattgag tacgcgaaga aatataacgt accggtggtg ctgacgctgg gcaccaagtt
    36661 tgtcattgcc gagaatccgc agtggtggca gcaattcctc aaagatcacg tctctatcct
    36721 tgcgatgaac gaagatgaag ccgaagcgtt gaccggagaa agcgatccgt tgttggcatc
    36781 tgacaaggcg ctggactggg tagatctggt gctgtgcacc gccgggccaa tcggcttgta
    36841 tatggcgggc tttaccgaag acgaagcgaa acgtaaaacc cagcatccgc tgctgccggg
    36901 cgctatagcg gaattcaacc agtatgagtt tagccgcgcc atgcgccaca aggattgcca
    36961 gaatccgctg cgtgtctatt cgcacattgc gccgtacatg ggcgggccgg aaaaaatcat
    37021 gaacactaat ggagcggggg atggcgcatt ggcagcgttg ctgcatgaca ttaccgccaa
    37081 cagctaccat cgtagcaacg taccaaactc cagcaaacat aaattcacct ggttaactta
    37141 ttcatcgtta gcgcaggtgt gtaaatatgc taaccgtgtg agctatcagg tactgaacca
    37201 gcattcacct cgtttaacgc gcggcttgcc ggagcgtgaa gacagcctgg aagagtctta
    37261 ctgggatcgt taagttatcg tcggttcgta ggccagataa ggcgttcacg ccgcatctgg
    37321 catttggctc tcgatgcctg atgcgacgct ggcgcgtctt atcatgccta catatttttc
    37381 atattttaca tccggcaacc accgtttacc ccgtcaccac ttcacccgcc ggtggcgttt
    37441 ccagcagttc cagcatagta cgggcaattt cacgttcacc catcactacc tgattcgcgc
    37501 cacgttcggt gatatacgtc acttcatcgt cataatgggc gcgggcgata atctcaatat
    37561 ccgggttttt cgcgcgggca gacgccacaa tctcacccgc ttcataaccg ttggggatcg
    37621 tcaggatcag ccattttgca cattccagat gcgccagttg cataatttct tcgttcgctg
    37681 cattgcccaa tacagcgcgg accccacgct ctcgcagctc atcaacacgg gttcgtgacg
    37741 tctcaatcac caccagcgga atatcagagg cgagcaattt ctcccccagc aggctgccta
    37801 cacgaccgta acccaccaat agcgcatggt tgcaaatatc cactgggatc tgtttctctt
    37861 cttcgattgc ctcttccagc gtctgctctt ccagcgtttc ggtcttcgcc agatatttct
    37921 ccagtagtgc gaacagtacc gggttgagca taatcgacag gatcgcccct gccagtacca
    37981 ggttttgtcc ggcctgcggc agtaaattca atgccattcc cagtcccgcc aggataaacg
    38041 cgaactcacc aatctgcgcc aggctggcgg cgatggttaa tgccgtacgt tgggagtgac
    38101 caaacagacg gacaaggaac agggccgcga ccgatttacc aaacagaata attgccagcg
    38161 tcgccagcac tgccagcggt tgctgaatca gaattaacgg atcaaacaac atcccgacgg
    38221 agacaaaaaa cagcaccgca aacgcgtcgc gcaatggcag cgtatcgtgg gcggcacggt
    38281 gactcagttc agactcgttc agtaccatcc cggcaaagaa cgcgccaagt gcgaaggaga
    38341 cgtcaaacaa ctccaccgca ccaaaggcaa tccctaacgc cagcgccagc accgacaggg
    38401 taaatagctc gcgagaaccg gttgccgcgc tgcgtgccat aatccacggc accagacggc
    38461 gacctaccag catcataatg gcgataaatg cgatcacttt gccgatggtg atccccatat
    38521 cgactgcaag agtggcaaag cccacatcgc cctgttccat cattcctgcc actgcgggca
    38581 gcaacaccag cgtcagaacc attaccaggt cttccacaat caaccaaccg atggcgattt
    38641 gcccacgctg actgtcaatt aattgccgtt cttcaagtgc gcgcagtaac accacggtac
    38701 tggcggtgga aagacataaa ccgaacacga taccggtcat taacgaccag cccagcacgg
    38761 cagagagcgc catacccagc agcgtcgcca cggctatctg ggcgatcgca ccgggaatgg
    38821 cgatggcctt taccgccatc aaatccttca gcgaaaagtg caaaccgacg ccaaacatca
    38881 acagaatgac gcccagttca gccagttccg gcgcaagctt ggtatcggca acaaagcccg
    38941 gagtgaatgg tcctgccagc acacccgcta acagatatcc caccagagga gaaatacgta
    39001 gtttattggc cagcatgccg aggataaagg cgagcacaag gccgccaaca atggtggtga
    39061 taagcggggt ggcgtgatgc attccgtctc cttttcctgg tggttattgt ccatttttgg
    39121 ccgggaaaac caaaattaca ggtaatagtt tatgacaatt tcattgatga tgttcatgaa
    39181 taattattga attttgcaga aaaatggaat tagctgcaaa aaaagcacgg atcggaaaac
    39241 agaaggcggt ttaacagagg aaaggcttat ggcgactgta aaacgatgca gccaaagttt
    39301 gcctttggct gcaatgaaat cagtctttat gccggttatc aggcaggaat atggtcaaca
    39361 tccccaatag tggcaggaaa gcacagattt tatagactaa ctcgatg
//
LOCUS       DAJVQG010000018        39258 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJVQG010000018 DAJVQG010000000
VERSION     DAJVQG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39258
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.018"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="OQM83_000881"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="OQM83_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCS1519206.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="OQM83_000882"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="OQM83_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCS1519207.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="OQM83_000883"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="OQM83_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCS1519208.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="OQM83_000884"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="OQM83_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCS1519209.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="OQM83_000885"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="OQM83_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCS1519210.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDISARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLVQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="OQM83_000886"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="OQM83_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCS1519211.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="OQM83_000887"
     CDS             complement(6574..7338)
                     /locus_tag="OQM83_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1519212.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="OQM83_000888"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="OQM83_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCS1519213.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="OQM83_000889"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="OQM83_000889"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCS1519214.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="OQM83_000890"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="OQM83_000890"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCS1519215.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            12023..12718
                     /gene="araD"
                     /locus_tag="OQM83_000891"
     CDS             12023..12718
                     /gene="araD"
                     /locus_tag="OQM83_000891"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCS1519216.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12793..15144
                     /gene="polB"
                     /locus_tag="OQM83_000892"
     CDS             12793..15144
                     /gene="polB"
                     /locus_tag="OQM83_000892"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCS1519217.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15308..18214
                     /gene="rapA"
                     /locus_tag="OQM83_000893"
     CDS             15308..18214
                     /gene="rapA"
                     /locus_tag="OQM83_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCS1519218.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRMEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18226..18885
                     /gene="rluA"
                     /locus_tag="OQM83_000894"
     CDS             18226..18885
                     /gene="rluA"
                     /locus_tag="OQM83_000894"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCS1519219.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="OQM83_000895"
     CDS             complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="OQM83_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCS1519220.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            20072..22426
                     /gene="lptD"
                     /locus_tag="OQM83_000896"
     CDS             20072..22426
                     /gene="lptD"
                     /locus_tag="OQM83_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCS1519221.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22479..23765
                     /gene="surA"
                     /locus_tag="OQM83_000897"
     CDS             22479..23765
                     /gene="surA"
                     /locus_tag="OQM83_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCS1519222.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            23765..24754
                     /gene="pdxA"
                     /locus_tag="OQM83_000898"
     CDS             23765..24754
                     /gene="pdxA"
                     /locus_tag="OQM83_000898"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCS1519223.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24751..25572
                     /gene="rsmA"
                     /locus_tag="OQM83_000899"
     CDS             24751..25572
                     /gene="rsmA"
                     /locus_tag="OQM83_000899"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCS1519224.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25575..25952
                     /gene="apaG"
                     /locus_tag="OQM83_000900"
     CDS             25575..25952
                     /gene="apaG"
                     /locus_tag="OQM83_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCS1519225.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25959..26801
                     /gene="apaH"
                     /locus_tag="OQM83_000901"
     CDS             25959..26801
                     /gene="apaH"
                     /locus_tag="OQM83_000901"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCS1519226.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26879..27358)
                     /gene="folA"
                     /locus_tag="OQM83_000902"
     CDS             complement(26879..27358)
                     /gene="folA"
                     /locus_tag="OQM83_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCS1519227.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="OQM83_000903"
     CDS             complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="OQM83_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCS1519228.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="OQM83_000904"
     CDS             complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="OQM83_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCS1519229.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(30043..31374)
                     /locus_tag="OQM83_000905"
                     /pseudo
     CDS             complement(30043..31374)
                     /locus_tag="OQM83_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="OQM83_000906"
     CDS             complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="OQM83_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCS1519230.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31717..33003)
                     /locus_tag="OQM83_000907"
     CDS             complement(31717..33003)
                     /locus_tag="OQM83_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1519231.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(33054..33995)
                     /locus_tag="OQM83_000908"
     CDS             complement(33054..33995)
                     /locus_tag="OQM83_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCS1519232.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(34010..34780)
                     /locus_tag="OQM83_000909"
     CDS             complement(34010..34780)
                     /locus_tag="OQM83_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCS1519233.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35253..36767
                     /gene="caiT"
                     /locus_tag="OQM83_000910"
     CDS             35253..36767
                     /gene="caiT"
                     /locus_tag="OQM83_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCS1519234.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            36798..37940
                     /gene="caiA"
                     /locus_tag="OQM83_000911"
     CDS             36798..37940
                     /gene="caiA"
                     /locus_tag="OQM83_000911"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCS1519235.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            38069..39258
                     /gene="caiB"
                     /locus_tag="OQM83_000912"
                     /pseudo
     CDS             38069..39258
                     /gene="caiB"
                     /locus_tag="OQM83_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
BASE COUNT         9395 a        10791 c        10280 g         8792 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatctccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggtg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caagagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaatgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcatc cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg
    12001 aaacgacctc tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa
    12061 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    12121 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    12181 tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    12241 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat
    12301 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag tcgggtcagt cgattccagc
    12361 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    12421 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    12481 tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc
    12541 gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    12601 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    12661 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    12721 actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga
    12781 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg
    12841 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    12901 gcaccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt
    12961 ttgcagggta aacaaggctt tcgcctgaca ccgctggcgt taaaggattt tcaccgccag
    13021 ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg
    13081 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    13141 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt
    13201 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat
    13261 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc
    13321 atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa
    13381 tacgtcgcca gccgcccgca gttgctggaa aaactcaacg cctggtttgc caactacgat
    13441 cctgatgtga tcatcggttg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat
    13501 gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc
    13561 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    13621 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    13681 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    13741 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    13801 gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca
    13861 acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc
    13921 tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    13981 cacaccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    14041 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    14101 gggctggttg aaggcatggc gcagcctgat ccagagcaca gtaccgaagg ttttctcgat
    14161 gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg
    14221 cgcgatgaag ccaaacgtca gggtaataaa ccactttcgc aggcgctgaa gatcattatg
    14281 aatgcctttt atggcgtgct tggcaccacc gcctgccgct ttttcgatcc gcgcctggca
    14341 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcatt gattgaagcg
    14401 cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca
    14461 cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    14521 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact
    14581 catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc
    14641 tacgccgggc tgattcagga gggcgataaa cagcggatgg tgtttaaagg gctggaaacc
    14701 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctata cctgcgcatc
    14761 ttccgcaacg agccatatca ggaatatgta cgcgaaacca tcgacaaact gatggcgggt
    14821 gaactggatg cgcgactggt ttaccgtaaa cgccttcgcc gtccgctgag cgagtatcag
    14881 cgtaatgtgc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgt
    14941 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    15001 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    15061 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    15121 accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg
    15181 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    15241 ccactacccc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    15301 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    15361 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    15421 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    15481 tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    15541 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    15601 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    15661 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    15721 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    15781 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    15841 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    15901 cgctgctgaa cgtgtgctaa ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    15961 aatgctgcgc cgtttcaacc tgcgctttgc gctatttgat gatgagcgtt atgccgaagc
    16021 tcagcacgat gcttacaacc cgttcgacac cgaacagctg gtgatttgct cgctggattt
    16081 tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg acctgctggt
    16141 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    16201 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    16261 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    16321 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    16381 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    16441 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    16501 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    16561 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    16621 gccgctgccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    16681 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    16741 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    16801 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    16861 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    16921 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    16981 ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    17041 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    17101 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    17161 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    17221 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccggt
    17281 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    17341 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    17401 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    17461 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    17521 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    17581 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    17641 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg atactggtag
    17701 cagcacgatt tcactattga aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    17761 gatttatgtc gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    17821 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    17881 tgaaaccttt aaccgtcagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    17941 cgccgtgcag caggatgttc acgctatcct tcagctgggt gaagcgcaga tcgagaaatc
    18001 tgcccgtgca ttgattgatg ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct
    18061 gtctcgtatg gaagcgctgc gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    18121 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga
    18181 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    18241 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca cattatggtg
    18301 gttaacaagc cgagcggttt gttgtctgtg ccggggcgtc tggaagagca caaagacagc
    18361 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    18421 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    18481 cagttccgcg agcgcgagcc gaaaaagcag tatgtagccc gcgtctgggg gcatccatcc
    18541 cctgcggaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    18601 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    18661 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcagctgcgt
    18721 gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    18781 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    18841 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    18901 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    18961 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    19021 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    19081 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    19141 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    19201 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    19261 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    19321 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    19381 agtgaaccat cagcaaacgc cgcctgaatc tggatttcca gaaacatacg aattaagtca
    19441 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    19501 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    19561 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    19621 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    19681 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    19741 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    19801 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    19861 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    19921 ttgcattatc atacctgtca ttcaatgtcg tgcctaacac cactgacgct attcggacag
    19981 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    20041 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    20101 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    20161 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    20221 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    20281 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    20341 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    20401 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    20461 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    20521 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    20581 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    20641 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    20701 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    20761 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    20821 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    20881 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    20941 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    21001 ttggttgttc tactggcaac actccggggt catggatcaa gtgtggcgtt tcaacgtcga
    21061 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    21121 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    21181 caccgtttca accaagcagt tccaggtctt tagcgagcag aacaccagta gctactcggc
    21241 agagccgcag ttagacgtta actactacca gaatgatgtt ggtccgtttg atacgcgtat
    21301 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    21361 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    21421 agcgaagttg ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    21481 cacgaccaag ctggacgaat ccgttaaccg cgttatgccg caattcaaag ttgacggcaa
    21541 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    21601 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    21661 gtctctactg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    21721 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    21781 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    21841 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    21901 cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    21961 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    22021 tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta cgctgcctaa
    22081 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    22141 ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    22201 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    22261 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    22321 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    22381 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    22441 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    22501 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    22561 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    22621 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    22681 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    22741 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    22801 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacacct
    22861 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    22921 gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    22981 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    23041 cttctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    23101 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    23161 gcggccagat gggctggggc cgtattcagg agttgccggg tatcttcgcc caggcattaa
    23221 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    23281 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    23341 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgt gtgaaactgg
    23401 aacagattgc tgccgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    23461 tctctcagga tccaggctct gctaaccagg gcggtgatct cggctgggct acaccagata
    23521 ttttcgatcc ggccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    23581 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    23641 aaaccgacgc tgcgcagaag gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    23701 aagaagcagc aagctggatg caggaacaac gcgccagcgc ctacgttaaa atcctgagca
    23761 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    23821 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    23881 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    23941 ttcccccaac tcccctgcac aaccgcaaac tgcgggcaca ttaacgctac ttcctgtcgc
    24001 gctacgcgaa tctgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    24061 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    24121 ccccgtgcat aaaggcgtca ttaacgacgc aggtattccg tttactggtc ataccgagtt
    24181 tttcgaagag cgctcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    24241 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    24301 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    24361 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    24421 tacggaagag atagacacca ttattccggt gctcaacgag ctgcgggcgc aggggatgaa
    24481 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    24541 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    24601 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    24661 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    24721 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    24781 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    24841 tctgccatta acccgcaaaa gggccaagca atggtcgaaa tcggccccgg tctggcggca
    24901 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    24961 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    25021 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    25081 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    25141 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    25201 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    25261 atcccagtgc tggaagtacc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    25321 gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    25381 agccgcatca ccactgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    25441 aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat gcgagcggaa
    25501 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    25561 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    25621 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    25681 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    25741 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    25801 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    25861 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    25921 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    25981 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccctg
    26041 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg
    26101 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    26161 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    26221 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc
    26281 tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg ccgcagtggg
    26341 atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg agtgactcct
    26401 atcccttctt tctcgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    26461 gggggctggg aagactgcgt tttatcacca acgcttttac ccgtatgcgt ttttgcttcc
    26521 cgaacggtca actggatatg tacagcaaag aatcgccgga agaggcccct gccccactga
    26581 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    26641 gggcatcgct ggagggcaaa ggtacgccgg aaggtatata cgcgctggat accggctgct
    26701 gctggggtgg tacattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    26761 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatataggaa
    26821 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaaatt
    26881 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatca gcatcgtgga
    26941 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    27001 ccacttctgc gtcgatatgc gtcagataca gtttctgcgc ttttggcagg aactgctcat
    27061 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
    27121 catccaccga cttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    27181 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    27241 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    27301 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    27361 agatttcccg ataaaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    27421 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctctccct
    27481 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    27541 cactctttat taggatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    27601 accttcttcg gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    27661 ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    27721 gtcgttctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    27781 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagtgcca gacgcccggt
    27841 tttcagcgca ccttcgaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    27901 aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc aaatgcggga agtgttcttt
    27961 caccatctct gtcagttgca ggttggtttg cggatcgtcg atggcgttaa tcagcacttc
    28021 cgctttcgcc gctcccgcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    28081 tttcatacca aatttacgca aggtttcgat atggtccgga tcgtgatcga gcaccaccat
    28141 tttcaccccg ctggagagca gtaaacgtcc ggtgatctgc ccaaaacgac cgaatccggc
    28201 aatgattacc cgcggctgtt cttcgtcgat ctcatcagct tcacgcgcct cttcagtaga
    28261 agattgctca aggcgattga ggatcaccag cagaatcggc gttgccgcca tcgacagcgc
    28321 caccgccagg gtcagcgatt tcgcccactc cggttccagc acattcgcca tctgcgccgt
    28381 gccaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accaacgacg
    28441 ctgtttattt ggcacttgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    28501 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaagcagcg tgccaaagtc
    28561 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa atggttcgat
    28621 atcgctctcc agcgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc
    28681 gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacgaggaa
    28741 taacgccacg gcactaaaca cttcccgcaa gccagagcgg gctacaaaac gcagcgccgg
    28801 acgcgtgaca tagcgcccca gcaataccac cagcaccagc gcacccgcca cttttaacgc
    28861 cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gttgccagta gcggaatcat
    28921 cgccaccagc gggatcgccg cgatatcctg gaacagcagc accgcaaagg cactgcgacc
    28981 catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgtagagga
    29041 gagcgccagc gtcatgccga tcaattccgc gacctgccag tgcagcccaa gtaacatgca
    29101 gaacagcccc agcaggccgc cgcaaatcac catctgcaat gcgccaccgc cgaacactgc
    29161 cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag
    29221 caccacccca atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc
    29281 ccacgggcca ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg
    29341 taccgcaatg ggtacaatca gcgctgccga accgagataa atcagcgcct gaatcagcgt
    29401 atggctatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag tgacgcgcct
    29461 gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc
    29521 agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg
    29581 gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat
    29641 atttgccatg cagcgccttg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat
    29701 cgatccaaag tttaagaagc ggaggaatgc tgtaccactg catcggatgc tgccagacga
    29761 tcagatcggc gcgagacagc gcctcctgct cggcggcaat atcgatattg aagtcaggat
    29821 agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc
    29881 gtttattcgc atgggaatga tgcggatacg gatgcgcata aattataaga atcataggta
    29941 gcccgcgtga ttacgccttt gttttcccac aaagtgtagt catatattca cgcggcttat
    30001 agtcaataat attgattcag aaaagagaga aatctgaaca atttacccca ttctctgccc
    30061 gcggatcgtc atattgctgg tttgcgccag tgacatccct cgagtctccg gggcaaacgc
    30121 tacggaaatc aacaagccaa acagcgagat acccgcctcc attagcatcg tgttactgat
    30181 accgtaatta ttgataaaga tcggcagtgc ccaggtcgaa acaatggtgc caatacggct
    30241 taaggacata atcacgccca cggcagaggc gcggatatct gtcgggaaga gttcattagg
    30301 atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg caaaggccat
    30361 cactaccagc cagatcccca tatccgggat cagccccaaa accgccagcg ccagcgtcat
    30421 catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttaacc acagcatcgg
    30481 cggaatacag ccgagcataa agaacaggct aatcaccacg ttccccagcg ccgcgttttt
    30541 gccaacaccc aatcccaaca aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat
    30601 tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa aatggcggcg
    30661 attaaacaga tcgcgaaaac gggtttgcta cggctgctct tcatcgaaag ccaccggttc
    30721 gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc gtaataacca
    30781 gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc aacaaacagg ggatcgccgc
    30841 gctacccagc atccagcgcc agccgccttc cacatcataa agccagtagc cgaccagatc
    30901 ggcgcaggtc gcgccgacat accacatggc ggcgataaag ctgatggaaa atgcccgctg
    30961 acgggtactg gagaactcgg tgatcattga ggtggcgatg ggataatctg caccgatgac
    31021 aatgccgata agtacccgca tcaccaacag ttcgacgggg gatgaaacaa acatcgtcgc
    31081 caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc gtcctacttt
    31141 atcggaaata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc
    31201 caacaagcca atccagtcag cgtccagttt tagcgccgga gtcagttgct ccagcgccac
    31261 gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc acagcaaaat
    31321 gcggcggtga attgaggaga atttgagatc gtcaaagttt ctggacggtt gcatgggtta
    31381 ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa catcagccgt
    31441 aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca
    31501 gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc
    31561 cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagggcct
    31621 gtttatcggc atcggccttt acaacaatgt gcggatgctc ttcatcgaca ttgaatttat
    31681 tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat
    31741 gccatccttg atcagattga tgaagcccac tttcttgccg tggcggagga ttttcttgcg
    31801 catcagttcc ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag
    31861 ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaag gccggtagtt tctggtacat
    31921 acgcatatcg cgcagcgggc cactctcaag atgctgacga tattccgcca gtttttgctt
    31981 actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcggctt ccccggcggc
    32041 aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc
    32101 accggcaatc aatacgccgt caccaaccaa ctccggcagc atgttgatgc ctgcttccgg
    32161 cactacgtga gcggaatatt ccaccagctt gccgcccgcg atcagcggtg ccactgccgg
    32221 atgctgtttg aaatcttcca gcatttgcgg caccgatttt tttgcgtcat gcagatgatg
    32281 cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc
    32341 catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat taccctgcaa
    32401 ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc
    32461 cacatccgtc ggtttaacgc gttttgccat ccccagcttt tcggcaagga tggagttcac
    32521 tccatcagca aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac
    32581 gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgcgcc
    32641 cgcttcttcg gcctgctcca tcagccaggc atcaaattta ctgcgcaaaa cggagtaaga
    32701 acgctgggat ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc
    32761 cgtcataaac gcgagttttt catgggtgat caggcgttct accggggcgg attcggcgaa
    32821 accaggaata atgtgttcca ggctgtgggc atagagacgc ccgccggtga cgttcttggc
    32881 acctgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    32941 tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg gcgtcaaaga tatcttcgga
    33001 cataagagct ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt ggatcaacgc
    33061 gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt caccaacaat gccgtaatcc
    33121 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgatgca
    33181 ttagcaccaa ccatgtgctg gatctgcccg gagatcccca ccgccaggta cagttcaggt
    33241 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttcc
    33301 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc
    33361 gcaatgttct ctttgctgcc aataccgcga ccgacgctga ccaccagacg ggctttgtcg
    33421 agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga
    33481 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    33541 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    33601 gccagaccac cgtaaaccat atgtttcact gttgctttac cgtcctgtac gctgacggtg
    33661 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    33721 ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca
    33781 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    33841 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggtg
    33901 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta
    33961 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    34021 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    34081 gctgacgttc gcgctgtttc ggcgcggcaa cctgttgttc tgaccaggct gcctctgcgt
    34141 taaaaccaat atccgccgcc gaccatacct ggacgggctt tttcgccgcg ccgagaatgg
    34201 ctttcatcga aggaatttgt ggggaattga tatcagtgga aacagcaaca accgcaggca
    34261 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    34321 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    34381 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcaaat
    34441 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    34501 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agtacatctt
    34561 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggccgtc acctgcgcct
    34621 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttcagatcg tattggctta
    34681 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    34741 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    34801 aggaaacatc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    34861 taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt
    34921 gttaagttcc tcaccaatat tgaaaacatg gcgtagaaaa aagaaatttt caatattgct
    34981 ttatggatca ccaatattga aagtcagatg ctattttttt actcacaaca caacataaca
    35041 aactgattaa taatcaaaat taacgagaaa agcgccctct attcacccga aaaaaaatgt
    35101 gataacaatc acagaataca gcttattgaa tacccattat gagttagcca ttaacgcgtc
    35161 cacgaggtta ataataatta tatcaaatgt taacaaaaat aaaacaaacg ggaacgcaaa
    35221 ataaatattc gttttcacag tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa
    35281 tagaaccgaa ggttttcttt ccgccgttaa taatcgtcgg catactttgt tggcttacag
    35341 tcagagatct ggatgcagcg aatgtcgtta ttaatgctgt attcagttac gtcaccaatg
    35401 tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggttgg ttctggctgg
    35461 tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc acctgaattt agtaccgcca
    35521 gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc tggggatcga
    35581 ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac tcgacagggg
    35641 cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg tgggccactt
    35701 acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc
    35761 gccccagctc gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg ttgttcggca
    35821 ctatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc
    35881 ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt tggcattccg cataccctgc
    35941 aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc gtcgcttgcg
    36001 gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc
    36061 tgggttgggt gttcattgtc agcggtgcca gcttcatcat gaactacttc accgattcgg
    36121 tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc atcgctaaag
    36181 gcggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt tatgctatcc
    36241 agatgagtat cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa ctgtgcttcg
    36301 gcatggtgct ggggctgaca gcgtcaacct ggatcctgtg gactgtactc ggtagtaaca
    36361 ctctgctgtt gatagataaa aacatcatca acattccaaa tctgatcgaa cagtacggtg
    36421 tggcgcgcgc catcattgaa acctgggccg ctctgccact cagcaccgcc accatgtggg
    36481 gcttcttcat cctctgcttt attgccaccg ttacactggt taacgcctgc tcttataccc
    36541 tggcgatgtc cacttgccgc gaagtacgcg atggtgaaga accacctctg ctggtgcgta
    36601 tcggttggtc aattctggtt ggcattatcg gtattgttct gctggcgctc ggcggcctga
    36661 aaccgattca aaccgccatt atcgccggag gatgcccgct gttcttcgtc aacattatgg
    36721 tgacgctctc ctttattaaa gacgcgaaac agaactggaa agattaatta acccccaaaa
    36781 tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg tttgtcgccg
    36841 gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag tgcgaccgtg
    36901 acagcgtcta cccggaacgt tttgtcaaag cactggcgga tatgggtatc gacagtctgc
    36961 tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc gccgtgtgga
    37021 tggagctggg acgtctgggg gcaccaacct atgtgctgta ccagttgccg ggcgggttca
    37081 acaccttcct gcgcgaaggc acacaagagc agatcgacaa aattatggct ttccgcggca
    37141 ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta
    37201 gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt agtaagtgtt
    37261 ttattaccag cagcgcctac accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc
    37321 cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc atcaaagtga
    37381 ccaaacttga aaagctcggt ctgcgtatgg atagctgctg tgaaatcacc tttgacgacg
    37441 tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac cgcgtcaaag
    37501 aagagttcga ccatgaacgt ttcctggtag ccctcaccaa ctacggtacg gcgatgtgcg
    37561 cttttgaaga tgcggcgcgc tacgccaatc agcgcgtgca gtttggcgag gctattggtc
    37621 gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac tccatgaaaa
    37681 acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct ggcgatgcag
    37741 cgatgtgcaa atacttctgc gccaatgcgg catttgaagt tgtggatagc gcaatgcagg
    37801 tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg cgtgatctgc
    37861 gtgtagaccg cgtctccggg ggatctgacg aaatgcagat cctgacgctg ggtcgcgcgg
    37921 tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc gacgctaatg cgtcttatca
    37981 ggcctacggg agcacgaatg taggccggat aaagcgttta cgccgcatcc ggcagtcatg
    38041 cactacataa atttttcagg agctaataat ggatcatcta cccatgccga aattcgggcc
    38101 gttggccgga ttgcgcgttg tcttctccgg tatcgaaatc gccgggccgt tcgccgggca
    38161 aatgttcgca gaatggggtg cggaagttat ctggatcgag aacgtcgcct aggccgacac
    38221 cattcgcgtt caaccgaact acccgcagct ctcccgccgc aatttgcacg cgctgtcgtt
    38281 aaatattttc aaagatgaag gccgcgaagc gtttctgaaa ttaatggaaa ccaccgatat
    38341 cttcatcgaa gccagcaaag gtccggcctt tgcccgtcgt ggcattaccg atgaagtact
    38401 gtggcagcac aacccgaaac tggttatcgc tcacctgtcc ggttttggtc agtacggcac
    38461 cgaggagtac accaatcttc cggcctataa caccatcgcc caggccttca gtggttacct
    38521 gattcagaac ggtgatgttg accagccaat gcctgccttc ccgtataccg ccgattactt
    38581 ttctggcctg accgccacca cggcggcgct ggcagcactg cataaagtgc gtgaaaccgg
    38641 taaaggcgaa agtatcgaca tcgccatgta tgaagtgatg ctgcgtatgg gccagtactt
    38701 catgatggat tacttcaacg gcggcgaaat gtgcccgcgc atgagcaaag gtaaagatcc
    38761 ctactacgcc ggttgcggtc tgtataaatg cgccgacggc tacatcgtga tggaactggt
    38821 gggcattacc caaattgaag agtgctttaa agatattgac ctcgcacatc tactcggtac
    38881 gccggaaatc ccggaaggca ctcagcttat ccaccgtatc gaatgccctt acggcccact
    38941 ggtagaagaa aaactcgatg cctggctggc ggcacatacc atcgcagaag ttaaagaacg
    39001 ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc gtaccggaac tggaaagcaa
    39061 tccacagtat gttgcccgtg aatcaatcac tcagtggcaa acgatggatg gtcgcacctg
    39121 caaagggccg aacatcatgc cgaaattcaa aaataacccc ggacaaatct ggcgcggaat
    39181 gccctcacat ggcatggaca cggctgccat tttgaaaaat atcggctaca gcgaaaacga
    39241 cattcaggag ttggtcag
//
LOCUS       DAJVQG010000019        38126 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJVQG010000019 DAJVQG010000000
VERSION     DAJVQG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38126
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.019"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            11..85
                     /locus_tag="OQM83_000913"
     tRNA            11..85
                     /locus_tag="OQM83_000913"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43..45,aa:Gln,seq:ctg)
     gene            complement(239..1414)
                     /gene="ubiF"
                     /locus_tag="OQM83_000914"
     CDS             complement(239..1414)
                     /gene="ubiF"
                     /locus_tag="OQM83_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCS1519236.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1560..2984
                     /gene="miaB"
                     /locus_tag="OQM83_000915"
     CDS             1560..2984
                     /gene="miaB"
                     /locus_tag="OQM83_000915"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCS1519237.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3137..4177
                     /locus_tag="OQM83_000916"
     CDS             3137..4177
                     /locus_tag="OQM83_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCS1519238.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4174..4641
                     /gene="ybeY"
                     /locus_tag="OQM83_000917"
     CDS             4174..4641
                     /gene="ybeY"
                     /locus_tag="OQM83_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCS1519239.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4731..5609
                     /gene="corC"
                     /locus_tag="OQM83_000918"
     CDS             4731..5609
                     /gene="corC"
                     /locus_tag="OQM83_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCS1519240.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5634..7172
                     /gene="lnt"
                     /locus_tag="OQM83_000919"
     CDS             5634..7172
                     /gene="lnt"
                     /locus_tag="OQM83_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCS1519241.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(7250..9127)
                     /locus_tag="OQM83_000920"
     CDS             complement(7250..9127)
                     /locus_tag="OQM83_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCS1519242.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVPAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            9582..10490
                     /gene="gltI"
                     /locus_tag="OQM83_000921"
     CDS             9582..10490
                     /gene="gltI"
                     /locus_tag="OQM83_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCS1519243.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            10660..11400
                     /gene="gltJ"
                     /locus_tag="OQM83_000922"
     CDS             10660..11400
                     /gene="gltJ"
                     /locus_tag="OQM83_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCS1519244.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            11400..12074
                     /gene="gltK"
                     /locus_tag="OQM83_000923"
     CDS             11400..12074
                     /gene="gltK"
                     /locus_tag="OQM83_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCS1519245.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            12074..12799
                     /gene="gltL"
                     /locus_tag="OQM83_000924"
     CDS             12074..12799
                     /gene="gltL"
                     /locus_tag="OQM83_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCS1519246.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            12917..13852
                     /gene="rihA"
                     /locus_tag="OQM83_000925"
     CDS             12917..13852
                     /gene="rihA"
                     /locus_tag="OQM83_000925"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCS1519247.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            13944..15614
                     /gene="hscC"
                     /locus_tag="OQM83_000926"
     CDS             13944..15614
                     /gene="hscC"
                     /locus_tag="OQM83_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCS1519248.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(15674..17128)
                     /gene="djlC"
                     /locus_tag="OQM83_000927"
     CDS             complement(15674..17128)
                     /gene="djlC"
                     /locus_tag="OQM83_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCS1519249.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDDEEEREAFLEA
                     IQAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAK
                     LHRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLC
                     AYWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTR
                     LLWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVV
                     LEAFPKQEMLARLNTRLEGGDTHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKN
                     DEDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(17125..17832)
                     /locus_tag="OQM83_000928"
     CDS             complement(17125..17832)
                     /locus_tag="OQM83_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS1519250.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEERKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            17934..18488
                     /locus_tag="OQM83_000929"
     CDS             17934..18488
                     /locus_tag="OQM83_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS1519251.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(18498..19925)
                     /locus_tag="OQM83_000930"
     CDS             complement(18498..19925)
                     /locus_tag="OQM83_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCS1519252.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLLSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(19922..20629)
                     /locus_tag="OQM83_000931"
     CDS             complement(19922..20629)
                     /locus_tag="OQM83_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS1519253.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
     gene            20793..21769
                     /locus_tag="OQM83_000932"
                     /pseudo
     CDS             20793..21769
                     /locus_tag="OQM83_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
     gene            complement(21839..22321)
                     /locus_tag="OQM83_000933"
     CDS             complement(21839..22321)
                     /locus_tag="OQM83_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCS1519254.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            22556..25138
                     /gene="leuS"
                     /locus_tag="OQM83_000934"
     CDS             22556..25138
                     /gene="leuS"
                     /locus_tag="OQM83_000934"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCS1519255.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            25153..25734
                     /gene="lptE"
                     /locus_tag="OQM83_000935"
     CDS             25153..25734
                     /gene="lptE"
                     /locus_tag="OQM83_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCS1519256.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            25734..26765
                     /gene="holA"
                     /locus_tag="OQM83_000936"
     CDS             25734..26765
                     /gene="holA"
                     /locus_tag="OQM83_000936"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCS1519257.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVTARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            26767..27408
                     /gene="nadD"
                     /locus_tag="OQM83_000937"
     CDS             26767..27408
                     /gene="nadD"
                     /locus_tag="OQM83_000937"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCS1519258.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            27432..28043
                     /locus_tag="OQM83_000938"
                     /pseudo
     CDS             27432..28043
                     /locus_tag="OQM83_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            28303..28620
                     /gene="rsfS"
                     /locus_tag="OQM83_000939"
                     /gene_synonym="rsfA"
     CDS             28303..28620
                     /gene="rsfS"
                     /locus_tag="OQM83_000939"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCS1519259.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            28624..29091
                     /gene="rlmH"
                     /locus_tag="OQM83_000940"
     CDS             28624..29091
                     /gene="rlmH"
                     /locus_tag="OQM83_000940"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCS1519260.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            29122..31023
                     /gene="mrdA"
                     /locus_tag="OQM83_000941"
     CDS             29122..31023
                     /gene="mrdA"
                     /locus_tag="OQM83_000941"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCS1519261.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            31026..32138
                     /gene="mrdB"
                     /locus_tag="OQM83_000942"
                     /gene_synonym="rodA"
     CDS             31026..32138
                     /gene="mrdB"
                     /locus_tag="OQM83_000942"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCS1519262.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            32149..33237
                     /gene="rlpA"
                     /locus_tag="OQM83_000943"
     CDS             32149..33237
                     /gene="rlpA"
                     /locus_tag="OQM83_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCS1519263.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            33376..34587
                     /gene="dacA"
                     /locus_tag="OQM83_000944"
     CDS             33376..34587
                     /gene="dacA"
                     /locus_tag="OQM83_000944"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCS1519264.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            34697..34960
                     /locus_tag="OQM83_000945"
     CDS             34697..34960
                     /locus_tag="OQM83_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCS1519265.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            35061..35702
                     /gene="lipB"
                     /locus_tag="OQM83_000946"
     CDS             35061..35702
                     /gene="lipB"
                     /locus_tag="OQM83_000946"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCS1519266.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            35962..36915
                     /locus_tag="OQM83_000947"
     CDS             35962..36915
                     /locus_tag="OQM83_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCS1519267.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            37124..38089
                     /gene="lipA"
                     /locus_tag="OQM83_000948"
     CDS             37124..38089
                     /gene="lipA"
                     /locus_tag="OQM83_000948"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCS1519268.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT         9555 a         9730 c         9569 g         9272 t
ORIGIN      
        1 gtgcaccagt tggggtatcg ccaagcggta aggcaccgga ttctgattcc ggcattccga
       61 ggttcgaatc ctcgtacccc agccacatta aaaaagctcg cttcggcgag ctttttgctt
      121 ttctgcgttc attcaatgtc gaatgcgatg ttgacacgtc ttatccttca atgtcggatg
      181 cgacgctgcc gcgtcttatc cgacctacgg ttggcacgca tccggcaatg ttgtaaggct
      241 acaaccctaa cgcatatttc agcgcctgac gtttcaacac gccagcacgc tctgccgcca
      301 ttaacccgag attacgcaca aaacgcagcg gcggcagatt attactgaat ccggcataaa
      361 acagatccat accactttgc ataatgaagt tatccgccat gcgccgcatc tggtaacgct
      421 tgaggacagg ataactggcc cacgcttcac cgtagctgcg ggcattcacc agaacatcaa
      481 tcagggcatc gacatcacga taaccaagat tcaccccctg ccccgccagc ggatggatgg
      541 tatgcgcggc atcgcccacc agcgcaagcc ccggctgcac atactgcaac gcatgtcggc
      601 gcgtcagcgg aaacgcacca gcggcaagcg gtgtaacgta acccagacgc gacgggaaat
      661 gcttcgcgat ttccgcctgg aattgcgcca tattcatatt ctgcaactgg cgaatacgcg
      721 ccggagagtc ataccacacc agcgatgccc agttatcaaa caacggcaga aacgcacgcg
      781 gtccgtccgg ggtaaattgc tgccaggtgc tgtcgccggg atcgttctca cactgtacgc
      841 taatcaacat gcacgactgc gcatactgcc aggcatgaac gccaattccc gccatctgcc
      901 gcacctgaga atttgcgccg tcggcaccaa tcaccagttt cgcgcggatc gtttcaccgc
      961 ccttcagttc cagctcctga agatcattat ctcgatgtaa tgcaatcagc gagcctggca
     1021 cacgtaacgt tactttcgga tgcgcttcca gcgcctgcca cagcgcctgt tgcaggacgg
     1081 tgttttccac catatagcca agcaacggta gcttaagttc agcggcgtca aacaccacat
     1141 gcgccgtttc ccactcccac gtttccagtc tgcggtaagg atggcaacgc atagcctgta
     1201 ctgcatccca gacccctaac cctttaagca atgataccga tgcggcgctg atcgccgaga
     1261 tccgcacgtc cggttggcta tcagcgacaa acggtgcagg ttctgcgtgc tcgatcaccg
     1321 ttaccgaaaa tccgtgctgt gccagcccca gcgccagcgc gccgccgacc attcctccgc
     1381 cgacaatggc aatttccgtt ggttgatttg tcatggtcaa tcatcctgtt agcaatatgc
     1441 tttaagttta caggattttt acccccaagg gctgataacg ctcatactgg tcacaacggc
     1501 agcaaagcat tacactatac gccctgcatt cctggctact atttcgcaag agcaagtcga
     1561 tgaccaaaaa actccatatt aaaacctggg gctgtcagat gaacgagtac gattcatcga
     1621 agatggccga tctgctggat gccacccacg gctatcaact gaccgacgtg gcggaagaag
     1681 cggatgtgct gctgctgaac acctgctcaa tccgcgagaa ggctcaggaa aaagtcttcc
     1741 atcagttggg tcgctggaaa ctgttaaaag agaagaatcc agacctgatt atcggcgtcg
     1801 gtggctgcgt ggcatcgcaa gaaggcgagc acattcgcca gcgcgcccac tatgtcgata
     1861 ttatttttgg gccgcaaacg ctgcaccgtc tgccggagat gatcaactcc gtgcgcggcg
     1921 accgcagccc ggttgtagat atcagcttcc cggaaatcga gaagtttgac cgtctaccgg
     1981 aaccgcgcgc cgaagggccg accgcgtttg tctccatcat ggaaggctgc aataaatatt
     2041 gcacctactg cgtggtgcct tacacccgtg gtgaagaggt aagccgtccg tctgacgata
     2101 ttctgtttga gattgcccag cttgcggctc agggcgtgcg tgaagtcaac ctgctcggtc
     2161 agaacgtgaa cgcctggcgt ggtgaaaact acgacggctc caccggatcg tttgccgatc
     2221 tgctgcgtct ggttgctgcg atcgacggga tcgatcgtat tcgctttacc accagccatc
     2281 cgatcgaatt caccgacgat atcatcgaag tgtatcgcga cacgccggag ctggtgagct
     2341 tcctgcatct gccagtacaa agcggttccg atcgcattct gaacctgatg ggccgtactc
     2401 acacggcgct ggagtacaaa gcgatcatcc gtaaactgcg tgcggcgcgt ccggatattc
     2461 agatcagttc tgacttcatt gttggcttcc ctggcgaaac caccgaagac ttcgagaaaa
     2521 cgatgaagct gattgccgac gtcaatttcg acatgagcta cagctttatc ttctctgcac
     2581 gtccgggtac accagccgcc gatatggttg atgatgttcc ggaagaagag aagaagcaac
     2641 gtctgtatat tctgcaagaa cgtatcaatc agcaagcgat ggcatggagc cgccggatgc
     2701 tcggcaccac ccagcgtatt ctggtagaag ggacttcgcg taagagcatc atggagcttt
     2761 ccgggcgtac cgaaaataac cgcgtggtca acttcgaggg cacaccggat atgatcggta
     2821 aattcgtcga tgtagaaatt accgacgtct acccgaactc tctgcgcggt aaagtggtgc
     2881 gtactgaaga tgaaatgggt ctgcgcgtgg cagaaacacc ggaatcggtg attgcccgta
     2941 cccgcaaaga aaacgacctt ggcgtgggtt attatcagcc gtaattctca ggcccgccgt
     3001 tccggtgggc cttgtttttt ctctctttat ccccatcttt atcgcaatgc ttgcgccttt
     3061 ttcctgtggc gtgaataatt tccttatagg ccggtgaatg ttcagcttcc ggcggcatac
     3121 agataagagg aacggtttga acatagacac tcgcgaaatc accctggagc cagcagacaa
     3181 cgcgcgtctg ttgagcctgt gcggcccgtt tgatgacaac atcaagcagc tcgaacgccg
     3241 tctcggcatc gagatcaatc gccgcgacaa ccactttaaa ctgaccggcc gtccgatttg
     3301 cgtcaccgct gcggcagaca ttctgcgtag cctgtatgtc gatacagccc cgatgcgcgg
     3361 tcagattcag gatatcgaac cggaacagat ccaccttgcg attaaagaag cgcgagtact
     3421 ggagcaaagt gcggagagcg tgccggagta cggcaaagcg gtcaatatca aaaccaaacg
     3481 cggcgtaatt aagccgcgca cgccaaacca ggcgcagtac atcgccaata ttctcgacca
     3541 tgacattacc ttcggcgttg gcccggcggg tacgggtaaa acctacctgg cagtggctgc
     3601 ggcagttgat gccctggagc gtcaggagat tcgccgtatt ctgctgactc gtccggcggt
     3661 cgaagccggt gagaaactgg gcttcctgcc tggcgattta agccagaaag tagacccgta
     3721 tctgcgccca ctgtacgacg cgctgtttga aatgctgggc tttgagaaag ttgagaaact
     3781 gattgagcgc aacgttattg aagtcgcgcc gctggcctat atgcgtggtc gtacgctgaa
     3841 cgacgcgttt atcattctcg atgagagcca gaacaccacc atcgaacaga tgaagatgtt
     3901 cctgacccgt atcggtttta actcaaaagc ggttatcacc ggcgacgtca cacagatcga
     3961 cctgccgcgt aatactaaat caggcttacg tcacgccatc gaagtgctgg ccgatgtcga
     4021 agagatcagc tttaacttct tccacagcga agacgtggtt cgtcaccctg tggtggcgcg
     4081 tatcgttaac gcctatgaag cctgggaaga agccgaacaa aaacgaaaag cggcgctggc
     4141 ggcagaacgc aagcgcgaag aacaggaaca aaaatgagtc aggtgatcct cgatttacaa
     4201 ctggcatgtg aagataattc cgggttaccg gaagagagcc agtttcaaac atggctgaat
     4261 gcggtgatcc cgcagtttca ggaagaatcg gaagtgacta ttcgcgtggt cgataccgcc
     4321 gaaagccaca gtctgaatct gacctatcgc ggtaaggata agccgaccaa cgtgctctcc
     4381 ttcccgtttg aagtgccgcc tggcatggaa atgtcgctac tgggcgatct ggttatctgc
     4441 cgtcaggtgg ttgagaagga agctcaggag caaggcaaac cactggaggc gcactgggcg
     4501 catatggtgg tgcacggcag tctgcatttg ttaggttacg atcacatcga agatgacgaa
     4561 gcagaagaaa tggaagccct cgaaacagag attatgcttg ctctgggcta tgaggatccg
     4621 tacattgccg agaaagaata atttgccagg catttgactg gcagacgtcc ccgccgttgg
     4681 tgattaacga cgacgttttg attaactaac atgagaaccc atacgacgcc atgagcgacg
     4741 acaattcaca cagtagtgac acgataagca acaagaaggg atttttctcc ctgttactca
     4801 gccaactttt ccacggtgaa ccgaaaaacc gtgacgaact gctggcgctg atccgtgatt
     4861 ccgggcagaa cgaccttatc gacgaagata cgcgcgatat gctcgaaggg gtgatggaca
     4921 tcgcagacca acgcgttcgc gacatcatga tcccccgctc ccagatgatt accctgaaac
     4981 gcaaccagac gctggacgaa tgccttgatg tcatcatcga gtccgcccac tcacgtttcc
     5041 cggtgattag cgaagacaaa gatcacattg aagggattct gatggcgaaa gacttgctgc
     5101 cgtttatgcg cagcgatgct gaagccttca gcatggacaa agtgttacgt caggcggttg
     5161 tcgttcctga aagtaagcgc gtagaccgga tgctgaaaga gtttcgctct cagcgttacc
     5221 acatggcgat cgtcattgac gaattcggtg gggtttccgg tctggtgacc attgaagaca
     5281 tcctggaact gattgttggt gagattgaag acgagtatga cgaagaagat gatatcgact
     5341 tccgtcagct gagtcgtcat acctggaccg tgcgtgcact ggcttccatt gaagacttca
     5401 acgaagcgtt cggcacccac tttagcgatg aagaggttga cactatcggt ggtctggtga
     5461 tgcaggcatt tgggcatctt ccggcgcgtg gcgaaactat cgacatcgac ggttaccagt
     5521 tcaaagtggc gatggccgac agtcggcgta ttattcaggt tcatgtcaaa atcccggatg
     5581 actcacccca gccgaagctg gatgaataaa accgaaactg gatagataac tacatggctt
     5641 ttgcctcatt aattgaacgc cagcgcattc gcctgctgct ggcgttatta ttcggtgcct
     5701 gcggaacgct ggccttctct ccttacgacg tctggcctgc ggcgattatt tcgttgatgg
     5761 ggcttcaggc gttgaccttt aaccgccgtc cactccagtc tgccgctatt ggcttttgct
     5821 ggggatttgg cctctttggc agcggtatta actgggtcta tgtcagcatc gcgacctttg
     5881 gcggaatgcc tggcccggtt aacatcttcc tggtggtgct gctggcggcg tatttgtcgc
     5941 tgtataccgg actgtttgct ggcgtgctgt cgcgtctgtg gccgaaaacc acctggctgc
     6001 gcgtggcgat tgccgcccct gctctctggc aagtgaccga gtttctgcgc ggttgggtac
     6061 tgacaggctt cccgtggtta cagttcggct atagccagat tgatggcccg ttaaaagggc
     6121 tggcaccgat aatgggcgtg gaagccatta acttcctgct gatgatggtt agtggcctgc
     6181 tggcactggc gttggtcaaa cgcaactggc gtccgctggt ggtggccgtc gtgctgtttg
     6241 ccctaccctt cccgctgcgt tacatccaat ggtttacccc gcaaccggag aaaaccattc
     6301 aggtttcgat ggttcagggc gatattccgc aatcactgaa atgggatgga gatcagcttc
     6361 ttaatacgct gaagatttac tacaacgcaa cggctccgct gatgggcaaa tcatcgttga
     6421 ttatctggcc ggagtcggcg ataaccgatc tggaaattaa tcagcaaccg ttcctcaaag
     6481 cactggacgg tgagttgcgc gataaaggta gctcgctggt aaccgggatt gtcgacgcgc
     6541 gtctcaataa gcagaaccgc tacgatacct acaacaccat catcacactg ggtaaaggtg
     6601 caccatacag ctacgaatca gccgatcgct ataacaaaaa ccatctagtg ccgtttggcg
     6661 agtttgtccc gctggagtcg attctgcgtc cgttagcacc gttctttgat ctgccgatgt
     6721 cgtcgttcag ccgtgggcca tatatccagc cgccgctgtc ggtaaatggt attgagctta
     6781 ctgcggctat ttgctacgag atcattctcg gcgagcaagt gcgcgataac ttccgcccgg
     6841 ataccgacta tctgctgact atctccaacg atgcgtggtt tggtaagtct attggtccat
     6901 ggcagcactt ccagatggcg cgaatgcgtg cgctggagct ggcgcgccca ctgttgcgca
     6961 gcaccaacaa cggcattacg gcggtgattg gcccgcaggg ggaggttcag gcgatgatcc
     7021 cgcagttcac ccgcgaggtg ttgaccacta atgtgacacc gaccaccgga ctcacaccgt
     7081 atgcacgtac cggcaactgg ccactgtggg tgctgacggc gctgtttggt tttgccgctg
     7141 tgttgatgag tctgcgagca cgtaaacgtt gatgtgcctg atgtatcctg catgagcaaa
     7201 acgtactttc tcattgagct ttggggcatg gttatcatgc ccctttttct tacggttcag
     7261 ggatacaagc cacattgcca tattggggat gacgaataat tgcctgcgga tcaagcgagc
     7321 gcgtcctgtc catttggatc aacccgttct tcgccatgac aaatattttt ctaccatccg
     7381 ctgaaaaacg aaggtgatta acgtagctgg caggataaac gccaataatc tgcccggcat
     7441 taatattgag caggatgaca taaccttgca ggccatatga caccaacgct aaatattgac
     7501 cgtccgggct aataccctca acggaataaa cgctcctgct acaccattgc gtcataatgc
     7561 tggcttgttg atcccctacg cttatacgtg aaatcccctg actatcggca aaaataacct
     7621 cgccgttttc aggtcgaaaa cgcatcgccg atatcgttcc acgcgcatca cctgcgggaa
     7681 agtcttcttt ctgatgcgtg atggtattga tccgcacgat gttacctctg cctgctcccg
     7741 gaaccagtaa tgttctttca tccggggaaa gtgccataac ccaggcgcct ggtttaccgc
     7801 tcgtccccgt cccgtcgtat ttctccagcg ggatatccgc cacatgctga taagtcacca
     7861 gatcaatcgc tggcacacta ttactggtgc gattcatcac ccaggctttt gtgccatcac
     7921 gcgagaggat aaggctgtca ggatgtgcac cggtggtaat cgactgaata atctcccctg
     7981 tcgccacgtt aatgaccgat aacgcattcg cttcaaatga agtcgcataa atcaactttt
     8041 catccgggct taatgccagc gatctgacgc cctgatcttt acagccacca caatgtttga
     8101 ctgacttttc tttcgctatg ggcgctgtaa aggtatgcag gattttcttt tgtccgaggt
     8161 cgaaaacaac aatgctgttc gtgttttcaa ttgcggcata caacatatta ccatcccgac
     8221 tgatcgccat atcatggata tcggcaatct cagcgagcaa atcaccactg gcctgttcac
     8281 gacttaccgg agcggataat gtttccctga gggcgttgcg ttcctccgct aactgctgct
     8341 ttttttgccg tcgctcttgt tcaatctcct gtggataaaa cgcttcacgc aggctttgcc
     8401 tgacctgtaa aactgactca tccatctgct gctgcgcaag ccagatagcc ccggtcagaa
     8461 gaagactgcc cgcaacaata atcccgcctt ccgtaacgag tcggttttgc ccagataaac
     8521 gcgcgctcag ccaacccaac gcgccacccg caatcaggcc gccaatgtgg caagcgttat
     8581 ctattcctgt ttgccgggca ccgtacaaca gtgtcagcgc caccataccc aacagaggaa
     8641 atactcgccg ctggttttta tggtatgtgc ccgtacctga agcaaactgt gttgctatcg
     8701 ctgcaccagc tattcccaga atcgccccgg atgccccaac gctgatgact atctgctcac
     8761 tttcgcgtaa cgcataacag gcgctgatga gcccaccaaa gacgccggag aataaccagg
     8821 taatcaggag tcgccatttc cccagaatct gttccgccac gcctcccacg accaggaatg
     8881 caaacatatt catcagcaga tgagaaacac tactgtgcag aaagatactg ctcaataagc
     8941 gccagggttc acccgacaaa gtaagaggcg caacgtttgc gccgagatgg ataagtgcgg
     9001 attgatcgac ctcttcataa ctcatccatt gtgcgagata acaaaatatc ccaatacaga
     9061 gtgcaaaaag gataagggtt attgcgggta attgaacatt taacggctta accgaagatg
     9121 cagacataag gtgtttcttc cttgaaaaga caggtaaaaa gcatttttcc cgtgtgcagt
     9181 tatcatcccg gcaaacgtcc catgccgaaa agataattct gtccgaaata tgactttttc
     9241 agcctaaaat tacaatcgta attaattcgc ccctaagtat tttttggcat gtaagttgct
     9301 ttatttactg tgtaagcgat gaaatcagcc gcgacgcaac caggttgcac caccacaaat
     9361 cagcggtagc accaaaaagg tgcggtttaa gaattttgct tcggaatggt gcggcgcatc
     9421 tcgcaaaaac aaacaataaa acagcaatat ctttacattt agccaaacta aatgttacct
     9481 aacaggcaca acactgcaca ataaagttgc agacgataac aacacaaaca ctcacaacgg
     9541 gtatccatgc gttcttaacg cagaagataa aggagttgga tatgcaatta cgtaaacctg
     9601 ccacagcaat cctcgccctg gcgctttccg caggactggc acaggcagat gacgccgccc
     9661 cggcagcagg cagtacgctg gacaaaatcg ccaaaaacgg tgtgattgtc gtcggtcacc
     9721 gtgaatcttc agtacctttc tcttattacg acaatcagca aaaagtggtg ggttactcgc
     9781 aggattactc caacgccatt gttgaagcag tgaaaaagaa actcaacaaa ccggacttgc
     9841 aggtaaaact gattccgatt acctcacaaa accgtattcc actgctgcaa aacggcactt
     9901 tcgattttga atgtggttct accaccaaca acgtcgaacg ccaaaaacag gcggctttct
     9961 ctgatactat tttcgtggtc ggtacgcgcc tgctgaccaa aaagggcggc gatatcaaag
    10021 attttgccga cctgaaagac aaagccgtag tcgtcacttc cggcactacc tctgaagttt
    10081 tgctcaacaa actgaatgaa gagcaaaaaa tgaatatgcg catcatcagc gccaaagatc
    10141 acagtgactc tttccgcacc ctggaaagcg gtcgtgccgt tgcctttatg atggatgatg
    10201 ccctgctggc cggtgaacgt gcgaaagcga agaaaccaga caactgggaa atcgtcggca
    10261 agccgcagtc tcaggaagcc tacggttgca tgttgcgtaa agacgatccg cagttcaaaa
    10321 agctgatgga tgacaccacc gcgcaggtgc agacctccgg tgaagcggaa aaatggtttg
    10381 ataaatggtt caaaaatccc attccgccga aaaacctgaa catgaatttc gaactgtcag
    10441 acgaaatgaa agcactgttc aaagaaccga atgacaaggc actgaactaa ttacaagaac
    10501 caggggcgga aattccagcc ctctcgattg ttacgtagca cggacagact atacgcctga
    10561 tggtcgttcc ccatcgggcc tgaaaaccgc aatacgctgg gtaacaatct tcgagggtag
    10621 cagttaacgc tgctaccctc ttttttctgg agtagattta tgtctataga ctggaactgg
    10681 ggtatttttt tacaacaagc cccgttcggc aacaccacct atcttggttg gatctggagt
    10741 ggttttcagg tgacgatcgc tttgtcgatc tgcgcctgga ttatcgcttt cctcgtcggt
    10801 tcatttttcg gcattttacg taccgttcca aaccgttttc tctccggtct gggtacgttg
    10861 tatgtcgaac tgttccgcaa cgtgccgttg attgtgcaat tctttacctg gtatctggtg
    10921 atcccggagc tgttgccgga gaaaatcggc atgtggttta aggccgagct ggatcccaat
    10981 attcagtttt tcctttcatc catgctctgc ctggggctgt ttaccgccgc ccgtgtttgc
    11041 gaacaggtac gcgccgccat tcagtcgttg ccgcgtgggc aaaaaaatgc cgcgctggcg
    11101 atgggcctga cgctaccgca ggcttatcga tacgtactgc tgcctaatgc ttatcgcgtt
    11161 atcgtcccgc cgatgacttc cgagatgatg aacctggtga aaaactccgc catcgcctcg
    11221 actattggtc tggtggatat ggcggcgcag gcgggtaaat tgcttgatta ctcagcccac
    11281 gcctgggagt cgtttaccgc tattacgctg gcgtatgtgt tgattaacgc ggttatcatg
    11341 ctggtaatga cgctggttga acgtaaagtt cgcctgcctg gcaacatggg gggcaaataa
    11401 tgtacgagtt tgactggagt tccattgtcc cttccctgcc atatctgctc gacgggctgg
    11461 tgatcacgct gaaaatcacc gtcacggcag tcgtgattgg tattttgtgg ggcaccatgc
    11521 tggcggtgat gcgcttgtcc agctttgcgc ccgtagcgtg gtttgccaaa gcctacgtta
    11581 acgtattccg ctcgattcct ttagttatgg ttttgctgtg gttttacctg atcgtgccag
    11641 gttttctgca aaacgtgctg ggattatcac caaaaaatga tatccgttta atttcggcga
    11701 tggtggcgtt ttcgatgttt gaagcggcct actattcaga gattatccgt gctggtattc
    11761 aaagtatttc tcgtggtcag tcgagcgccg cgctggcgtt gggaatgact cactggcagt
    11821 cgatgaaact gattattctg ccgcaggcgt tccgcgcgat ggtgccgctt ctgctcactc
    11881 agggcatcgt actgttccag gatacctcac tggtgtatgt gttatctctg gccgatttct
    11941 tccgtaccgc ctcaaccatt ggtgagcgtg atggtacgca ggttgagatg atcctgtttg
    12001 ccggatttgt ttatttcgtt attagtctta gcgcgtcgtt gttggtcagc tacttgaaaa
    12061 gaaggacagc ataatgatta ccctgaaaaa tgtttcaaaa tggtatggtc actttcaggt
    12121 gctgaccgac tgctcaaccg aagtgaaaaa aggcgaagtg gtggtggttt gcggcccgtc
    12181 tggttccggc aaatcaacgc tgattaaaac cgtcaacggc ctcgaaccag tgcagcaagg
    12241 tgaaatcacc gtcgatggta tcgtggttaa tgacaagaaa accgatctgg caaagctgcg
    12301 ttcccgcgtc gggatggtat tccagcattt cgagctgttc cctcatctgt cgattatcga
    12361 aaacctgacc ctggcgcagg tgaaagtgct taaacgcgat aaagcgcccg ctcgtgaaaa
    12421 agcattgaaa ttgctggagc gcgtgggact ttccgcacat gccaataaat ttccggcgca
    12481 gctttccggc ggtcagcagc agcgtgtggc aatcgctcgc gcattgtgta tggatcctat
    12541 tgcgatgctg ttcgacgaac cgacatcggc gctggatccg gagatgatca acgaagtgct
    12601 ggacgtgatg gtggaactgg cgaacgaagg aatgaccatg atggtggtga cccacgaaat
    12661 gggctttgcc cgcaaagtgg cgaatcgggt gatctttatg gacgagggta aaattgtcga
    12721 agactcgccg aaagacgctt tcttcgatga tccgaaatcg gaccgcgcga aagacttcct
    12781 cgcgaaaatc ctgcattaat cactctggcg cgcataccca agtgcgcgcc atctcacgct
    12841 ttatcgtttc gcccctcgta tctgttacat cctggcgtta cccttgtcgc aaagaagcac
    12901 acaacaagga gcaacaatgg cactgccaat tctgttagat tgcgacccag gtcatgacga
    12961 cgctatcgca atagttctcg ccctcgcctc accagagctt gatgtcaaag caattacgtc
    13021 ttccgccgga aaccagacac cagaaaaaac cttacgcaat gttctgcgta tgctgacctt
    13081 gcttaatcgc accgatattc cggtagcagg cggcgcggta aaaccgttaa tgcgtgagtt
    13141 gattatcgcg gacaatgtgc acggcgaaag cggtctggat ggcccggcat taccggaacc
    13201 gacgttcgca ccgcaaaact gtacggcggt agagctgatg gcgaaaacgc tgcgcgaaag
    13261 tgcggaacct gtcacgattg tgtctaccgg accgcaaact aacgttgcct tgctgctcaa
    13321 tagccatccg gaactgcata gcaaaattgc ccgtatcgtg attatgggcg gcgcaatggg
    13381 gcttggtaac tggacgcctg cggctgaatt taatatttac gttgacccgg aagcggcaga
    13441 aattgtcttc cagtcaggga tcccagtggt gatggccggt ctggatgtta ctcacaaagc
    13501 acaaatccac gttgaagaca ccgagcgttt ccgcgcgatt ggcaaccctg tttcaaccat
    13561 tgttgccgaa ctgctggatt tcttcctcga atatcataaa gacgaaaaat ggggctttgt
    13621 cggtgcaccg ctgcatgacc catgcaccat cgcctggctg ttgaaacccg agttgtttac
    13681 cactgttgag cgctgggttg gcgtggaaac acaggggaaa tatagccagg gtatgacggt
    13741 tgttgattat tattatctga ctggcaataa accgaatgcc actgtaatgg tcgatgttga
    13801 tcgtcagggc tttgttgatt tactggctga tcgtctgaaa ttttacgctt aacacacgtc
    13861 attgattaag atcaccacgc gttacctggg tggtcttaat ctgtttatct gtttatctct
    13921 ttttctttca agatataaat gcaatggata atgcagaact cgccattggt atcgatctcg
    13981 gcactaccaa tagtttaatt gccgtctgga aagacggtgc cgcgcaatta attccaaata
    14041 agttcggtga atatttaaca ccatccataa ttagcatgga tgaaaataat catattttag
    14101 tcggaaaacc ggctgtatca cggcgtactt cgcatccgga taaaacggca gcgttattta
    14161 aacgtgcaat gggcagtaat accaactggc ggttaggcag cgacacattt aacgcgccag
    14221 aactgtcctc tttggtatta cgctcattaa aagaagatgc cgaagaattt ctgcaacgtc
    14281 cgattaaaga tgtggtgatc tccgttccgg cttatttcag cgatgaacaa cgcaagcata
    14341 cccgtttagc agcggagtta gccgggttaa atgcggtacg cttaattaat gaacccacag
    14401 cagctgcgat ggcatatggc ctgcatacgc aacaaaatac ccgctcgctg gtttttgatc
    14461 tcggtggcgg cacgtttgac gtcacggtgc tcgaatacgc cacgccggtg attgaagttc
    14521 acgcctccgc tggcgataac ttcctcggtg gcgaagattt tacccatatg ttggtcgatg
    14581 acgttttaaa gcgcgcggat gtcgccagga ccacgcttaa cgagagtgaa ctggcagcct
    14641 tgtacgcctg tgtggaagcg gcaaaatgta gcaatcaatc gccattgcac attcgctggc
    14701 agtaccagga tgaaacgcgg gagtgcgaat tttacgagaa cgaactggaa gatttgtggt
    14761 tgccgctgct caatcgcttg cgagtgccga ttgaacaggc gttgcgcgat gcacgtctga
    14821 agccgagtca aatcgacagt ctggtgctgg ttggcggcgc gtcacaaatg ccgctggtgc
    14881 agcgaatcgc cgtgcgtctg tttggcaaat taccgtatca aagttacgac ccgagcacca
    14941 ttgtcgcgct gggcgcagca atccaggccg cctgccgctt acgcagtgaa gatattgaag
    15001 aggtgatcct cactgatatt tgcccttact cgttgggcgt tgaagttaac cgccagggcg
    15061 tttccggcat tttctcgcca attattgaac gaaacaccac agtacccgtg tcgcgtgtag
    15121 aaacttactc gaccatgcac ccggaacagg attctattac ggttaacgtc tatcagggag
    15181 aaaaccacaa agttaaaaat aacattctgg ttgaatcctt cgatgtgccg ttgaagaaaa
    15241 ccggggctta tcagtcgatt gatattcgct ttagttatga tatcaacggc ttgcttgaag
    15301 ttgacgtgct tctggaagat ggcagcgtta agtccagagt cattaatcat agcccggtaa
    15361 cattgagcgc gcagcagatt gaagagagtc ggacgcggtt atccgcattg aaaatttatc
    15421 cgcgcgatat gctaatcaac cgcaccttta aggccaaact ggaagagttg tgggcgcggg
    15481 cgctgggtga cgagcgagaa gagattggcc gggtgatcac cgattttgat gcggcgttgc
    15541 agtcaaacga tatggcccgc gtcgatgaag ttcgtcgacg ggcgagcgat tatttggcta
    15601 ttgagatccc ttaaacacgg ctgccggatg cggcataaac gctgcatccg gcatcggcgc
    15661 aaaaaacaca tcctcagcta aacaacatcg cgatttttcc gagcgcataa cctatcatca
    15721 gcaccgttaa gctgattttg ataatactgg taatgctctg tggtttttca tcctcatcat
    15781 ttttttcttc atggttttca ggtgttggct catcgacatc ttcatcctcc ggctcgagtt
    15841 tcactttcgt caacagcatc tgggtgataa tggcctctaa tggatgagta tctccacctt
    15901 ccagccttgt attaagacgg gccagcatct cttgtttcgg gaacgcttcc agcacaacct
    15961 gttccgctac ccgatacaaa tccagtaacg gtgaatcttg ctctgtctcg ctaccgactc
    16021 tcgatttcca ccacgcccat gctttgcgct tgaagaataa cgactcaata aacgattgac
    16081 ctaacggcga taattttccg ctatgtaaaa tctccgccca taacagccgc gtccgggcgc
    16141 tctcaccagg aacatatttc aacggtttac cttgcgcatc taccatgctt cgcgcttcga
    16201 taaccatcac taataacgcg aaataatccg ggcaatgacg cttgctccac tgcaacaaca
    16261 aattatcagc ttgtgtagat gggtaacttt cccagtacgc gcagagatcg gcaagcaggc
    16321 tttccccctc gccgcagtac aaacgttgtg cacactggta ataccgggca tcttcgcttt
    16381 ctggttcttc cgcttgccac tgtttggcta caggaatgag ttccgccata cctgtttgca
    16441 ccgagctgta ccagcgcaac agtttgcgat gtaactttgc atcatcagga atgaaccacg
    16501 gtccttccat cgccaaaaac gcagtgacat agtcaggatg gtaacggcag caacgttcca
    16561 gcgcgaagta atattcaaca gtctggttct gtaacgcgac gggatattcc agaaggctaa
    16621 ggaaatcgaa acaatcacca gcctgaatgg cctcaagaaa agcctccctt tcttcctcat
    16681 catccattcc ttcgctgtca ttaaacgaat gccagttcaa acgctctgcc aacaaagaag
    16741 cacaatttaa tgaaagatat cgcgcttcta tagcgattgc acacagcggc cagcgtaatt
    16801 gatcgacatc ctccatgttc caggtattta attgctgaat aaatttctgc caggcggagg
    16861 gctgaaaacg atcactttct gaatccagta atgtcctgaa tgcacgtagt atttcatgtt
    16921 cagcggtggc atctttttct tcatcatcgg cttcctcgac aggatttacc gccagtcgca
    16981 gcgcctcttc ataggcctgg cgtaatgctt taaacccctg cggatcggtt tcgggatgac
    17041 acaacggtaa gcgagcaaga taagcctggc ggataatgtc tatttgcgtc gtgctttcaa
    17101 tttcgaggat ttgccaacat gttttcatag gtcgctcaca tccttaagcg ttaccgggca
    17161 ttcaggctgg acagttaatg gttgccaggg aagcgtggca tagaaacggt cgcttcccgc
    17221 tgccagctct tcgaacatcc gactcccggc gctatcgtat ttgtacaggg ttagcgcctc
    17281 gcgtagcgaa gcgttgtctt cgcactgaga agattgccag tagagacgcc ctagtgaata
    17341 ggcagaaaaa tagtgcatcc agctgtgata gtaatggtga gctcgtgcat agacgcgcga
    17401 ttgcagccat aaactctctt cttccgtcag ccagttgttg agcacaccga cccgtgacag
    17461 aaaccccatt cgaacataat cccaggcttt gattccccct tctccacagc acatagcggt
    17521 ttgggcaacg aattgggtat aaatcaaccc tctctccgag ccaccagcaa ttaatgcttt
    17581 cctctcttca ggggaagagc gttgccatgt cagatataag ccagctaaat cattggcatg
    17641 accatcatca gtcatctgat aaatagtctg aattaaatca tcacgattat ttattcccca
    17701 ggatgatgag agatcgaatt cttttatccc agtatacatt tttggcgagt catgcgcggg
    17761 tttgcaactc tgattaagta tttccatggg cgcagaaagc gcaaaaagaa gatattgttc
    17821 ttccttattc attttttaat aatcccttaa gtgcattaac tgactacttt tttattattg
    17881 atgagtataa acttgacgct ttcaaaataa aaagaaaatc gaagcattca cacatgaata
    17941 aaaaatcaat gtatatattc gcaattttta tagttgcagc aattacctgt attagccaac
    18001 ccaagaaaac gacgttgcgt gataaagcca tggtgaatta tgcctttgat tatttaagat
    18061 caccaggcag tctaccgtta accacggcag ccacggagct ttccgcgatt catggtcact
    18121 caacatcgca atatcgcctt ggcgaatttt atcttcatgg tagcgacggt aaaccactgg
    18181 attatacaca ggcgagatac tggtatgagc aatcagcaga acaggaaaat ccgcgcgcac
    18241 aaagcaaact ggggtggatc tacctgaaag gcctgggagt caaacccgat acccgtaaag
    18301 caattctctg gtataaggaa gcagctgaac aagggtatgc tcatgctcaa tatactttgg
    18361 gtttgatcta cagaaatggc actggtatta atgttaacca ttatgaatct caaaaatggt
    18421 taaaactggc cgccaaacaa cattacaaaa atgcggaaag attacttgcc ggacttcccg
    18481 cacattaacg tcgactgtca cccgaagaga tggagtattt ttcctatcaa accggcaaag
    18541 ataaaaatgt agaaaataat tttgatgacg cccagacagc ccagcccgcc atcgtcaacg
    18601 ggcattggct gcggttgcgg ctccggctcg acggtttcct gaccggtaaa attcgccgcc
    18661 tgaattaata atcgacgaat aattaatgct tccagtggac aacgatcttc tgcatcaagg
    18721 cggctcacaa tcggcattaa tgattcttct gtcggttggc tggcctgcac taattgtttg
    18781 gcaactaaat ataatggcca taagggatgt tctgtatcac gcgtgagtga gatccgcggc
    18841 ttacgtcctt tgtgcaaaac catctcgacc agtgaacgcc ccagcgcaga gagtttttca
    18901 tcgctgagta tttcgtataa atgaaaacgc gtacgggcgc tgtcgccggg gacataaagt
    18961 agctgtttac cctgatcgtt aaccagttca cgagcttcaa tcatcatcac cactaacggg
    19021 taataatcga cccgatgctg acggcaccat tgcaatatta aagcatccgc ctgggtggag
    19081 ggatactcgc gccagtagtc acaaagttct gccagcaagc tttcgccttc tccacaatag
    19141 acgcgttgcg cgtattcata ataacgcgca tcttcattat caggttcagc ttcccgccac
    19201 gaccgggcgt aatccagaag ttcggggata tccaggcgac ctgcgctata ccagcggagt
    19261 aaatttctgt gcagttttgc atcatcgggg atgatcactg cgcgatgttg cgctaaatac
    19321 aatgtcagcc attctggatg cgatgaccaa atccgagcga acatttggta aaaatcgatg
    19381 gtgtcatttt gcacggcaac gggcagatgc agaatggtct ggaagttaaa aacattcccc
    19441 ttcgcaatgg catataaaaa ggattccagt tcttcttcgt ctatttcccc gacgttttcc
    19501 tcctgcaacc accgcaatct ttctgctaat aacaccacgc actcgaagga taaatgggca
    19561 gtgttcatgg cgattgtgca cagcgaccag cgtaattcat caatctcttc catcgagcaa
    19621 taatttaatt gctgaatgaa tcgctgccag gcggagggca gaaaacgttc gctatcagag
    19681 gcaagtaacg cgcgaaaggc gagcagaatt tcatgttctg ctacctcata ttcttctggt
    19741 tgccaaacag atttagcagg cgactgcgca atccggagcg cctcctcata cgcctgacga
    19801 agttgtttaa aaccctgcgg atcggtttct ggatgaaagg acagtaacag cgccagataa
    19861 gcgcggcgga taatatcgac gtcagtcgtt tcctctattt cgaggatctt ccagcaattt
    19921 ttcataggtc gctcatatcc ttaagcgttt ccgggcatgt ggggtagtca gccagtggtt
    19981 gccaggacaa ggcagggtaa tacgcatcat cactggcaaa taattgataa aacatatcat
    20041 tgccggaatc gtcgtattct ttacgagcta agaattcgcg taataaaggc agatgatcat
    20101 cttcgggaga ctgccaatat tgccgaccaa aggtataacc ggcaaagtat tgccgccagt
    20161 tactataata acgtagagcc cgtagatgaa tacgcgactg gatccataga ctttcttctt
    20221 cactcaacca gttattaagt acgcccattc gacttaaaaa acccatccgg acataatccc
    20281 atgctttaat tcctccctca ccacagcatt cggctgtgct ggcgacaaat tgcgcataag
    20341 cctgaccttg ctcatttagt tcagcaagat agtcacgcca ttcacatggc gaataacgaa
    20401 accagcggtg ataaaaccct gccaaccgag cggcatgacc gttatcgatc attcggtgaa
    20461 ttgtgcgcag caggtcatca cggtcatcaa ttccccatga ggaagaaagg tcaaaaaagc
    20521 atgtacctaa atacattttg ggggatgaat gcgaaggtaa acagcattca ttgcgtattt
    20581 ccatcggagt ggaaagtgca aacaatattt tttgcgaccc catgtccata taatttatcc
    20641 ttaaacattc atttaatgaa tgtaaactac atttcgagac tcagttattt atacttttct
    20701 gacgcaacat aatacgcata aaataaggct tacgcaaaaa tggaacacat aaaaatataa
    20761 tatgttttat ctacaaccat aaggaaatga taatggtttt tacgtcaagt tgctgcgata
    20821 atttatcaat agatgagatt atcgaacgtg ctgaaaaagg cgattgcgag gctcaatata
    20881 ttgttgggtt ttattataat cgcgatagcg caattgactc tccggacgac gaaaaggcct
    20941 tttactggct gaagctggcc gctgagcaag gtcattgtga agcacagtat tccttagggc
    21001 aaaagtatac cgaggataaa agccgtcata aagataatga gcaagccatc ttctggctga
    21061 aaaaagctgc cctacaaggc catactttcg cttccaacgc ccttggctgg acactggatc
    21121 gtggagaagc ccccaattat aaagaagcgg ttgtctggta tcagatagcc gcggagagcg
    21181 gaatgtctta tgcgcaaaat aatcttgggt ggatgtacag aaatggcaac ggagtcgcaa
    21241 aagactatgc gctggcattt ttctggtaca aacaagctgc attacaaggc catagtgacg
    21301 cgcaaaacaa tctggccgat ctttatgaag acggaaaagg cgttgctcaa aacaagacac
    21361 tcgccgcatt ctggtatttg aaaagcgcac agcagggtaa tcggcacgcc cagtttcaaa
    21421 ttgcatggga ttataacgct ggcgaagggg tggaccagga ctataagcaa gcgatgtact
    21481 ggtatctgaa ggctgccgct caggaaagcg tcggcgctta cgtcaacatc ggttatatgt
    21541 ataaacacgg acaaggcgtt gagaaagatt atcaggctgc ctttgaatgg tttacgaaag
    21601 ccgctgaatg caatgacgcc actgcctggt ataacctggc cattattatc actacggaga
    21661 aggaagacct gtcgatctcc gacaggctct cgacctgtat cgtaaagttc agtcatccgg
    21721 aaccagagat gtcagtcagg aaattcgtga gactgaagat ttactgtaga gataaaaaca
    21781 agggccgggg aatttcattc ggcccatatt ctctttattg ctgtgcgaaa tcagactatt
    21841 acggctcaaa cgggcgtctc tggaactggt catgaccaca tttcgggcat agcgtcagca
    21901 cttccggtgt gtagatcggg agatggaagt gacatttctc gcagaccaga tttcccagcc
    21961 cgaccacttc tccgctgtga taaaccccat gatgattgag gtcctggaaa acttcgcgcc
    22021 attcaagctg cgttttatcg gtgatatctg ccagctcctg ccataagctt tctttaatca
    22081 cccgcataaa gacgctgtca gattcttctt tcaggctctc ttcatagctc atggcgaact
    22141 cttccaggtc acgtcttaca gctcgcgtca gctcatcgac ctcggttcgc gttaactccc
    22201 ctgtttttat tacgcgctcg cgcgcctgtt ccaccagtgc gtcgatatca cgttcgccat
    22261 tgcgcaggcg ttcgctcagt gacgcgacca gttcacggta atattgagca accttgttca
    22321 tcgtttcatc tcccgggtcg ttaactgact ataataatag acgttctttg ttccttgctt
    22381 tgctaatacg cccacaaact ctgtaaatta atgtatgaat aatccgggca tcatttcaac
    22441 gtcgagtgcg cgaaaggctg ttttgacgcg gacgtttggg ctatgctatg cggatctgaa
    22501 aaaccacctc aacgctacat ttgtagccgt attgaaaaca ggaccactgg ctgccatgca
    22561 agagcaatac cgcccggaag agatagaatc caaagtacag cttcattggg atgagaagcg
    22621 cacatttgaa gtaaccgaag acgagagcaa agagaagtat tactgcctgt ctatgcttcc
    22681 ctatccttct ggtcgactac acatgggcca cgtacgtaac tacaccatcg gtgacgtgat
    22741 cgcccgctac cagcgtatgc tgggcaaaaa cgtcctgcag ccgatcggct gggacgcgtt
    22801 tggtctgcct gcggaaggcg cggcggtgaa aaacaacacc gctccggcac cgtggacgta
    22861 cgacaacatc gcgtatatga aaaaccagct caaaatgctg ggctttggtt atgactggag
    22921 ccgcgagctg gcaacctgta cgccggaata ctaccgttgg gaacagaaat tcttcaccga
    22981 gctgtataaa aaaggcctgg tatataagaa gacttctgcg gtcaactggt gcccgaacga
    23041 ccagaccgta ctggcgaacg aacaagttat cgacggctgc tgctggcgct gcgataccaa
    23101 agttgaacgt aaagagatcc cgcagtggtt tatcaaaatc actgcttacg ctgacgagct
    23161 gctcaacgat ctggataaac tggatcactg gccagacacc gttaaaacca tgcagcgtaa
    23221 ctggatcggt cgttccgaag gcgtggagat caccttcaac gttaacgact atgacaacat
    23281 gctgaccgtt tacactaccc gcccggacac ctttatgggt tgtacctacc tggcggtagc
    23341 tgcgggtcat ccgctggcgc agaaagcggc ggaaaataat cctgaactgg cggcctttat
    23401 tgacgaatgc cgtaacacca aagttgccga agctgaaatg gcgacgatgg agaaaaaagg
    23461 cgtcgatact ggctttaaag ctgttcaccc attaacgggc gaagaaattc ccgtttgggc
    23521 agcaaacttc gtattgatgg agtacggcac gggcgcagtt atggcggttc cgggtcacga
    23581 ccagcgcgac tacgagtttg cctctaaata cggcctgaac atcaaaccag ttatcctggc
    23641 tgctgacggc tctgagccag atctttctca gcaagccctg actgaaaaag gcgtgctgtt
    23701 caactctggc gaattcaacg gtcttgacca tgaagcggcc ttcaacgcca tcgccgataa
    23761 actgactgcg atgggcgttg gcgagcgtaa agtgaactac cgcctgcgcg actggggtgt
    23821 ttctcgtcag cgttactggg gcgcgccgat tccgatggtg acgctggaag acggtaccgt
    23881 aatgccgacc ccggacgacc agctgccggt gatcctgccg gaagatgtgg tgatggacgg
    23941 cattaccagc ccgattaaag cagatccgga gtgggcaaaa actaccgtta acggtatgcc
    24001 agcgctgcgt gaaaccgaca ctttcgacac ctttatggag tcctcctggt actatgcgcg
    24061 ctacacttgc ccagagtaca aagaaggtat gctggattcc gaagcggcta actactggct
    24121 gccggtggat atctacattg gtggtatcga acacgccatt atgcacctgc tctacttccg
    24181 cttcttccac aaactgatgc gtgatgcagg catggtgaac tctgacgaac cagcgaaaca
    24241 gttgctgtgt cagggtatgg tgctggcaga tgccttctac tatgttggcg aaaacggcga
    24301 acgtaactgg gtttccccgg ttgatgctat cgttgaacgt gacgagaaag gccgtatcgt
    24361 gaaagcgaaa gatgcggcag gccatgaact ggtttatacc ggcatgagca aaatgtccaa
    24421 gtcgaagaac aacggtatcg acccgcaggt gatggttgaa cgttacggcg cggacaccgt
    24481 tcgtctgttt atgatgtttg cttctccggc tgatatgact ctcgaatggc aggaatccgg
    24541 tgtggaaggg gctaaccgct tcctgaaacg tgtctggaaa ctggtttacg agcacacagc
    24601 aaaaggtgat gttgcggcac tgaacgttga tgcgctgact gaagatcaga aagcgctgcg
    24661 tcgcgatgtg cataaaacga tcgctaaagt gaccgatgat atcggccgtc gtcagacctt
    24721 caacaccgca attgcggcga ttatggagct gatgaacaaa ctggcgaaag caccaaccga
    24781 tggcgagcag gatcgcgctc tgatgcagga agcgctgctg gccgttgtcc gtatgcttaa
    24841 cccgttcacc ccgcacatct gcttcacgct gtggcaggaa ctgaaaggcg aaggcgatat
    24901 cgacaacgcg ccgtggccgg ttgctgacga aaaagcgatg gtggaagact ccacgctggt
    24961 cgtggtgcag gttaacggta aagtccgtgc caaaatcacc gttctggtgg acgcaacgga
    25021 agaacaggtt cgcgaacgtg ctggccagga acatctggta gcaaaatatc ttgatggcgt
    25081 taccgtgcgt aaagtgattt acgtaccagg taaactcctc aatctggtcg ttggctaagc
    25141 gcgggaggaa gcgtgcgata tctggcaaca ttgttgttat ctctggcggt gttaatcacc
    25201 gccgggtgtg gctggcatct gcgtgatacc acgcaggttc cttccactat gaaggtcatg
    25261 atcctggact caggcgatcc gaacgggcca ttaagccgtg cagtgcgtaa ccagttacgt
    25321 ctgaatggtg tcgagttgct tgataaagaa accacgcgta aggacgttcc atccctgcgt
    25381 ttgggtaaag tgagcatcgc gaaagatacc gcatcggtat tccgtaacgg tcaaacagca
    25441 gagtatcaga tgatcatgac ggtgaatgcg accgtgttga tccccggccg tgatatctac
    25501 ccgattagcg ccaaagtctt ccgttcgttc tttgataacc cgcaaatggc gttagcgaaa
    25561 gataacgaac aagacatgat cgtaaaagag atgtacgacc gtgctgccga acagctgatt
    25621 cgtaagctgc caagcatccg tgctgcggat attcgttccg acgaagaaca gacgtcgacc
    25681 acaacggata ctccggcaac gcctgcacgc gtctccacca cgctgggtaa ctgatgattc
    25741 ggttgtaccc ggaacaactc cgcgcgcagc tcaatgaagg gctgcgcgcg gcgtatcttt
    25801 tacttggtaa cgatcctctg ttattgcagg aaagccagga cgctgttcgt caggtagctg
    25861 cggcacaagg attcgaagaa caccacactt tttccattga tcccaacact gactggaatg
    25921 cgatcttttc gttatgccag gctatgagtc tgtttgccag tcgacaaacg ctattgctgt
    25981 tgttaccaga aaacggaccg aatgcggcga tcaatgaaca acttctcaca ctcaccggac
    26041 ttctgcatga cgacctgctg ttgatcgtcc gcggtaataa attaagcaaa gcgcaagaaa
    26101 atgccgcctg gtttactgct cttgcgaatc gcagcgtgca ggtgacctgt cagacaccgg
    26161 agcaggctca gcttccccgc tgggttactg cgcgcgcaaa acagctcaac ttagaactgg
    26221 atgacgcggc aaatcaggtg ctctgctact gttatgaagg taacctgctg gcgctggctc
    26281 aggcactgga gcgtttatcg ctgctctggc cagacggcaa attgacatta ccgcgcgttg
    26341 aacaggcggt gaatgatgcc gcgcatttca ccccttttca ttgggttgat gctttgttga
    26401 tgggaaaaag taagcgcgca ttgcatattc ttcagcaact gcgtctggaa ggcagcgaac
    26461 cggttatttt gttgcgcaca ttacaacgtg aactgttgtt actggttaac ctgaaacgcc
    26521 agtctgccca tacgccactg cgtgcgttgt ttgataagca tcgggtatgg cagaaccgcc
    26581 ggggcatgat gggcgaggcg ttaaatcgct taagtcagac gcagttacgt caggccgtgc
    26641 aactcctgac acgaacggaa ctcaccctca aacaagatta cggtcagtca gtgtgggcag
    26701 agctgaaagg gttatctctt ctgttgtgcc ataaacccct ggcggacgta tttatcgacg
    26761 gttgatatga aatctttaca ggctctgttt ggcggcacct ttgatccggt gcactatggt
    26821 catctaaaac ccgtggaaac gctggcgaat ttgattggtc tgacgcgggt cacaatcatc
    26881 cctaataatg ttcctccgca tcgtccccag ccggaagcga acagcgtgca gcgtaaacac
    26941 atgcttgaac tggcgattgc cgacaagcca ttatttactc ttgatgaacg cgagctaaag
    27001 cgcaatgccc cctcttacac tgcgcaaaca ctgaaagagt ggcggcagga acaaggaccg
    27061 gacgtgccgc tggcgtttat tattggtcag gattcactgc tgacctttcc gacctggtac
    27121 gaatacgaaa cgatactcga caatgcacat ttgatcgtct gtcggcgtcc aggttaccca
    27181 cttgaaatgg cgcaaccgca ataccagcaa tggctggaag atcatttgac acataacccg
    27241 gaagatcttc accttcagcc tgccggtaaa atttatctgg ctgaaacgcc gtggtttaac
    27301 atctcggcga ccatcatccg cgaacgtttg caaaacggtg aatcgtgtga ggatttattg
    27361 ccggaaccgg tactgactta cattaaccaa caaggcttgt atcgctgata cctgtcgtta
    27421 cggagggcat gatgcgactg tggttaattc gtcatggtga aacgcaagcg aatatcgatg
    27481 gtctttacag cggtcatgcg cccacccccc tgaccgcgcg cggtattgag caagcgcaaa
    27541 atctgcatac gctgctacat ggtgtttcct ttgatctggt tttatgcagt gaactggaac
    27601 gggcacagca taccgcacga ctggttctca gtgaccgcca gctccccgtg caaatcatcc
    27661 ctgaactcaa cgaaatgttt tttggcgact gggagatgcg acatcatcgc gacctcatgc
    27721 aagaagatgc cgaaaactat agcgcgtggt gcaatgactg gcagcatgca atccccacga
    27781 acggtgaagg atttcaggca ttttcgcaac gtgtggaacg ctttatcgca aggcttagtg
    27841 aatttcagca ctatcagaat attttagtcg tcagccatca gggtgtactg agtctgttaa
    27901 tcgcccgttt aattggcatg cctgccgagg ctatgtggca ttttcgcgtt gactaggggt
    27961 gttggagcgc cattgatatc aaccaaaaat tcgcgacgct acgcgtcctc aatagccgtg
    28021 ccatcggggt cgagaatgca tgacttttct gtttttttac gggtaagccg caacgaccat
    28081 tgacagaccc gggcaggctg atattctccg cagccagact tttttcgcca gacacgactt
    28141 tgtagaaatt gttttacaaa aatggcgatg caatctgcgg cgcggggtgg gatgatagcc
    28201 cactttcgaa agccgattcg gcgacaattg tcccgaaatc gcctctggtt caggtatact
    28261 gacagaccat ttttatctat ttgattcacc cagggggaaa acttgcaggg taaagcactc
    28321 caggattttg ttatcgacaa aattgatgac ctcaaaggtc aggacatcat cgccttagac
    28381 gttcaaggca aatccagcat caccgactgc atgatcatct gtacgggtac gtccagccgt
    28441 catgttatgt ccattgctga ccacgttgtg caggagtctc gcgcagcggg cctgttaccg
    28501 ctcggcgtag aaggtgaaaa cagcgccgac tggattgtcg tggatttggg cgatgtgatt
    28561 gtccatgtca tgcaggaaga gagccgtcgc ctgtatgaac tggaaaaact ctggagttaa
    28621 tgcgtgaagc tgcaacttgt cgccgtggga acgaaaatgc cggactgggt acaaaccggt
    28681 tttaccgagt acctgcgtcg ttttccgaaa gatatgccct tcgagctgat tgaaattccg
    28741 gccggaaaac gcggcaagaa tgcggacatc aagcgcatac tcgacaaaga gggtgagcag
    28801 atgttggcgg ccgcaggcaa aaaccgcatt gtcaccctcg atattccagg caagccctgg
    28861 gatacgccgc agttagccgc tgagctggaa cgctggaagc tggatggtcg cgacgtcagt
    28921 ctactgattg gcgggcctga agggttgtcg cctgcctgta aagcggcggc ggagcagagc
    28981 tggtcgctgt cggcgcttac cctcccccat ccgctggttc gcgtgctggt cgcagagagt
    29041 ctgtaccggg cgtggagcat caccaccaac catccttatc accgtgagtg ataagggagc
    29101 tttgagtaga aaacgcagcg gatgaaacta cagaactctt ttcgcgacta tacggcagag
    29161 tccgcgctgt ttgtgcgccg ggcgctggtc gcctttttgg ggattttgct gctgaccggc
    29221 gtgcttatcg ccaacctgta taatctgcaa attgttcgct ttaccgacta ccagactcgc
    29281 tctaatgaaa accgcattaa gctggtgcct atcgcgccca gccgcggcat tatctacgac
    29341 cgtaatggta tccctctggc cctcaaccgc actatctacc agatagaaat gatgccagag
    29401 aaagtcgata acgtgcagca aacgctggac gctttgcgca gcgtggttga tctgaccgat
    29461 gacgatattg ctgcattccg aaaagagcgc gcacgttcac accgtttcac ctctattccg
    29521 gtgaaaacta acctgaccga agtacaagta gctcgctttg ccgtcaatca gtaccgtttt
    29581 ccgggtgtcg aagttaaagg ctataaacgt cgttactatc cttacggttc ggcgttgacc
    29641 cacgtcatcg gctatgtgtc gaaaatcaac gataaagacg tcgaacgcct gaataatgac
    29701 ggcaaactgg ccaactatgc ggcaacgcat gatatcggta agctgggcat tgagcgttac
    29761 tatgaagacg tgctgcacgg tcagaccggt tatgaagagg ttgaagttaa caaccgtggg
    29821 cgtgttatcc gccagttaaa agaagtacca ccgcaagccg gacacgatat ttacctgacg
    29881 ctggatctca aactccagca atatattgaa acgctgctgg cgggtagccg cgcagctgtg
    29941 gttgtcaccg atccgcgtac aggtggggtg ctggcgctgg tttccacgcc tagttatgac
    30001 ccaaacctgt ttgttgacgg tatctccagc aaagattatt ccgccttgtt gaacgccccg
    30061 aatacaccgc tggtgaaccg cgccacacag ggggtttatc ctcccgcgtc tacagttaaa
    30121 ccctatgtgg cggtttcggc attgagcgcc ggggtgatca cgcgcaatac gacgctgttt
    30181 gacccaggct ggtggcaact gccaggttcg gaaaaacgtt atcgtgactg gaaaaaatgg
    30241 ggccacgggc gtctgaatgt cacaagatcg ctggaagaat ctgcggatac cttcttctat
    30301 caggtggcct acgatatggg gatcgatcgc ctctccgaat ggatgggtaa attcggttat
    30361 ggtcattaca ccggtatcga cctggcggaa gaacgttccg gcaacatgcc tacccgcgaa
    30421 tggaaacaga aacgctttaa aaaaccgtgg tatcagggtg acaccattcc ggttggtatc
    30481 ggtcagggtt actggacagc gaccccaatc cagatgagta aggcactgat gatcctgatt
    30541 aatgacggta tcgtgaaggt tcctcatttg ctgatgagca ccgccgaaga cggcaaacag
    30601 gtgccatggg tacagccgca tgaaccgccc gtcggcgata ttcattccgg ttactgggag
    30661 ctggcgaaag acggtatgta cggtgttgct aaccgcccta acggtacggc gcataaatac
    30721 tttgccagcg caccgtacaa aattgcggcg aaatccggta ccgctcaggt cttcggtctg
    30781 aaagcgaacg aaacctataa tgcgcacaaa attgccgagc gtttacgtga ccacaaactg
    30841 atgaccgcct ttgcgccata caacaatccg caagtggctg tcgccatgat tctggagaac
    30901 ggtggtgcgg gtccggcggt tggtacactg atgcgccaga tcctcgacca cattatgctg
    30961 ggtgataaca acaccgatct gcctgcggaa aatccagcgg ttgccgcagc ggaggaccat
    31021 taatcatgac ggataatccg aataaaaaaa cattctggga taaagtccat ctcgatccca
    31081 caatgctgct gatcttactg gcattgctgg tttacagcgc cctggttatc tggagcgcca
    31141 gcggtcagga tattggcatg atggagcgta aaatcggcca aatcgcgatg ggtctagtca
    31201 tcatggtggt gatggcgcaa attcctccac gcgtttatga aggctgggcc ccctatctct
    31261 atatcatctg tattattttg ctggtggcgg tagatgcttt cggtgccatc tctaaaggtg
    31321 ctcaacgctg gctggacctc ggtattgttc gttttcagcc gtcggaaatt gccaaaatag
    31381 ccgtaccact gatggttgcg cgctttatca accgcgacgt ttgcccgcca tcgttgaaga
    31441 acactggcat cgcgctggtg ctgatattta tgcccacgct gctggtggct gcacagcctg
    31501 acctgggaac atcaatcctc gttgcgcttt ccggtctgtt tgtactgttc ctctctggcc
    31561 ttagctggcg tctgattggc gtcgcagtag tgctggtggc ggcgttcatt ccgattctgt
    31621 ggttcttcct gatgcatgat taccagcgcc agcgcgtaat gatgctccta gacccggaat
    31681 cagacccact cggcgcgggc tatcacatta ttcagtctaa aattgctatt ggctccggcg
    31741 gattacgcgg caaaggctgg ctgcacggca ctcagtcaca gcttgaattt ctccccgaac
    31801 gccataccga ctttatcttc gcggtactgg cggaagagct gggattagtg ggcattctga
    31861 ttctgctcgc tctctacatt ctgctgatca tgcgcgggct gtggatagcc gccagagcgc
    31921 aaaccacctt tggtcgcgtc atggctggtg gcttaatgct gatattattc gtttatgtct
    31981 tcgtaaatat tggtatggta agcggtattc tgccggttgt aggggttccg ctcccactgg
    32041 tcagttatgg aggatcggcg ctaattgtgc tgatggctgg gttcgggatt gtaatgtcaa
    32101 tccacaccca caggaaaatg ttgtcgaaaa gcgtgtaaga ggtgcgcaat gcgtaagcag
    32161 tggctcggga tctgcatcgc ggcaggaatg ctcgcggcat gtacaagcga tgatggtcag
    32221 caacagacgg taagtgtacc gcagcctgcg gtatgtaacg gccccatagt tgaaattagc
    32281 ggggcggacc cgcgtttcga accactgaac gcgacggcaa atcaggatta ccagcgcgac
    32341 ggtaaaagct acaaaatcgt gcaggatccg tctcgattta gccaggcggg actggcggca
    32401 atctatgatg ccgaacccgg cagtaacctg acggcctctg gcgaagcttt cgatccgaca
    32461 cagctgacgg cggcccatcc aacgcttccg atccccagct acgccagaat cactaacctg
    32521 gctaacgggc gaatgatcgt ggtgcgcatt aatgatcgcg gcccttacgg caacgaccgc
    32581 gttatttcac tttctcgcgc ggcagctgac cgtcttaaca cgtcaaacaa caccaaagtt
    32641 cgtatcgatc cgattattgt cgcccaggat ggttcgcttt ctggtcctgg tatggcttgt
    32701 accacagtcg ccaaacagac ttacgccctg ccagcacctc ccgatttaag cggtggcgcg
    32761 ggaacaagtt cagtgtctgg cccgcagggt gacattcttc cggtcagtaa ttcgacgcta
    32821 aaaagcgaag atccgaccgg cgcgccggta accagtagcg gtttcctcgg cgctccaacg
    32881 accttagcgc ctggtgtact ggaaggcagc gaaccgacgc ctgcgccaca gcccgttgtt
    32941 acagctccgt cgacaacgcc tgcaacctcg cctgcaatgg tgacaccgca agccgcctcg
    33001 caaagcgcca gcggcaactt tatggtgcaa gtcggggccg taagcgatca ggctcgtgcg
    33061 caacagtacc aacagcaact gggacagaag ttcggcgtcc ccggtcgcgt aactcaaaat
    33121 ggcgcggtct ggcggatcca gcttggccca ttcgccagca aagccgaagc cagtaccttg
    33181 cagcaacgtt tgcaaaccga agcccaatta cagtcattta ttactaccgc gcagtagcgt
    33241 aaagcaggca tctgatgtgt caatttctgt aaatgacgtt aacaaagtca cgcggaaagt
    33301 cagatgcctg ccggtagtgc atttgctata gtagggcact tttttaattc catcacggat
    33361 gtcgtagttc agaccatgaa taccattttt tccgctcgta tcatgaagcg cctggcgctc
    33421 accacggctc tttgcacagc ctttatctct gctgcacatg ccgatgacct gaatatcaaa
    33481 actatgatcc cgggtgtacc gcagatcgat gcggagtcct acatcctgat tgactataac
    33541 tccggcaaag tgctcgccga acagaacgca gatgtccgcc gcgatcctgc cagcctgacc
    33601 aaaatgatga ccagttacgt tatcggccag gcaatgaaag ccggtaaatt taaagaaact
    33661 gatttagtca ctatcggcaa cgacgcatgg gccaccggta acccggtgtt taaaggttct
    33721 tcgctgatgt tcctcaaacc gggcatgcag gttccggttt ctcagctgat ccgcggtatt
    33781 aacctgcaat cgggtaacga tgcttgtgtc gccatggccg attttgccgc tggtagccag
    33841 gacgcttttg ttggcttgat gaacagctac gttaacgcac tgggcctgaa aaatacccac
    33901 ttccagacgg tacatggtct ggatgctgat ggtcagtaca gctccgcgcg agatatggcg
    33961 ctgatcggcc aggcattgat ccgtgacgta ccgaatgaat actcgatcta taaagaaaaa
    34021 gaatttacgt ttaacggtat tcgccagctg aaccgtaacg gcctgttatg ggataacagc
    34081 ctgaatgtcg acggcatcaa aaccggacac actgacaaag caggttacaa ccttgttgct
    34141 tctgcgactg aaggccagat gcgcttgatt tctgcggtga tgggcggacg tacttttaaa
    34201 ggccgtgaag ccgaaagtaa aaaactgcta acctggggct tccgtttctt cgaaaccgtt
    34261 aacccactga aagtaggtaa agagttcgcc tctgaaccgg tttggtttgg tgattctgat
    34321 cgcgcttcgt taggggttga taaagacgtg tacctgacca ttccacgtgg tcgcatgaaa
    34381 gatctgaaag ccagctatgt gctgaacagc agtgaattgc atgcgccgct gcaaaagaat
    34441 caggtcgtcg gaactatcaa cttccagctt gatggcaaaa cgatcgagca acgcccgctg
    34501 gttgtgttgc aagaaatccc ggaaggtaac ttcttcggca aaatcattga ttacattaaa
    34561 ttaatgttcc atcactggtt tggttaaaaa ttgaacactt gaaagtgtaa tttccgtccc
    34621 catatactaa gcatcagtaa aaaactcccg ccttctggcg ggagttgcta tttaattacg
    34681 ttacgccgga gctgacatga aaaccaaact taacgaactg cttgaattcc ctactccttt
    34741 tacttacaaa gttatggggc aggcgttacc tgagctggtt gatcaggtgg ttgaagtggt
    34801 acagcgccat gcgccaggtg actacacccc aacggtaaaa ccaagcagca aaggcaacta
    34861 ccactcggta tctatcacta tcaacgccac tcatatcgag caggttgaaa cactgtatga
    34921 agaactaggc aaaatcgata ttgtccgcat ggttctgtaa ctcgcttctc cgttacccgg
    34981 cccctggtcg ggtaactccc ctctcctcgc tgtgatatac ttttccccca cttttactca
    35041 ttctccacgg agatgcagtt ttgtatcagg ataaaattct tgtccgccag ctcggtcttc
    35101 agccttacga gccaatctcc caggctatgc atgaattcac cgatacccgc gatgatagta
    35161 cccttgatga aatctggctg gtcgagcact atccggtatt cacccaaggt caggcaggaa
    35221 aagcggagca cattttaatg ccgggtgata ttccggtgat ccagagcgat cgcggtgggc
    35281 aggtgactta tcacgggccg gggcaacagg tgatgtatgt gttgcttaac ctgaaacgcc
    35341 gtaaactcgg tgtgcgtgaa ctggtgacct tgcttgagca aacagtggtg aataccctgg
    35401 ctgaactggg tatagaagcg catcctcggg ctgacgcgcc tggtgtctat gtcggggaaa
    35461 agaaaatttg ctcactgggt ttacgtattc gacgcggttg ttcattccac ggtctggcat
    35521 taaacgtcaa tatggatctt tcaccatttt tacgtattaa tccttgtggg tatgccggaa
    35581 tggaaatggc taaaatatca caatggaaac ccgaagcgac gactaataat attgcaccac
    35641 gtttactgga aaatatttta gcgctactaa acaatcctga cttcgaatat attaccgctt
    35701 aattgcatac ctcaatggcc caatttacac tgggtcatta ctcataatac ctgctaatgc
    35761 tttacatctc cttcgcgttt gtatactttc tttatcactt gcagaaatgc ggtgctacgc
    35821 cttgctccgt aactcacccc attgattaac ctattgaaat agttgattat ggtgagcaaa
    35881 agcgttccgt tccaattaga cataagatta ttgtaatttt tttaaatatt atttttccat
    35941 gaataaaatt ggagtaagtg cgtggatagt aataatcaaa ttgaaccctg tttaagcaga
    36001 aagtcgtcag aaggtaaacc acaaatattt acaaccttac gaaatattga tcttaacctt
    36061 ctgactattt ttgaagctgt atatgtacat aaagggatcg ttaatgcagc gaaagtgctt
    36121 aatctgaccc cctcggcaat cagtcagtct attcagaaac tgcgcgttat attccctgac
    36181 ccattgttta ttcgcaaagg ccagggtgtc actcctaccg catttgcgat gcatctacat
    36241 gagtatatca gtcagggcct tgagtccatt cttggcgcgc tggatatcga aggaagctat
    36301 gataagcaac gaacgataac tattgcgacc actccctcgg tcggagccct ggtccttcct
    36361 gtcatctatc gggcgattaa aactcactat ccgcagcttt tactgcgcaa cccgcccatc
    36421 agcgacgcgg aaaaccaact cagtcagttt caaaccgatc tcatcatcga taacatgttt
    36481 tgcaccaatc gtacggtgca acatcatgtt ctgttcaccg acaatatggt gttaatttgc
    36541 cgcgagggaa atccactact ctctttagaa gatgacagag agactatcga caacgctgcg
    36601 catgtactcc tgttaccgga agggcaaaat ttcagcggtc tgcggcagag agttcaagag
    36661 atgtttccgg accggcaaat caatttcacc agctacaaca ttttgacaat cgctgcactg
    36721 gttgccaaca gtgacatgtt agcgattatt cccagccgtt tttataacct gtttagtcgt
    36781 tgctggccgc tggaaaaact gccttttccg tccttaaatg aggagcaaat agatttctct
    36841 atccactaca acaaattcag cctgcgtgat cccatcctgc acggggtaat agatgttatc
    36901 cgtaatgcat tttgaaaaag tattgttaca gccagatcac agcgcagaac gataaaatgg
    36961 cacaatccaa caacaatttt acattttcgc gaccgctttg gctgctttca ggtccgtttc
    37021 aatgatatac tgccagtcgt taattcaaaa atagttgata attacaacaa tctattgaat
    37081 tgaaacgctt tccttcgtaa ttcgcaactg gaacacgcac gctatgagta aacccattgt
    37141 gatggaacgc ggtgttaaat accgcgatgc cgataagatg gcccttatcc cggttaaaaa
    37201 cgtggcaaca gagcgcgaag ccctgctgcg caagccggaa tggatgaaaa tcaagcttcc
    37261 agcggactct acacgtatcc agggcatcaa agccgcaatg cgcaaaaatg gcctgcattc
    37321 tgtctgcgag gaagcctcct gccctaacct ggcggaatgc ttcaaccacg gcacagcaac
    37381 gtttatgatc ctcggcgcta tttgtacccg ccgttgtccg ttctgtgatg ttgcccacgg
    37441 tcgcccggta gctcctgatg ccaatgaacc agtgaaactg gcgcagacca ttgccgatat
    37501 ggcgctgcgt tatgtggtta tcacctccgt tgaccgtgat gacctgcgcg atggcggtgc
    37561 ccagcacttt gcggattgca ttactgccat tcgggaaaaa agcccgcaaa tcaaaattga
    37621 aactctggtg ccggatttcc gtggtcgtat ggatcgtgct ctggatattc tgactgcaac
    37681 gccaccagat gtgttcaacc ataacctgga aaacgtaccg cgtatttacc gtcaggtacg
    37741 gcctggtgca gattacaact ggtcgctgaa gctgctggaa cgctttaaag aagcgcatcc
    37801 ggaaatcccg accaagtctg gtctgatggt gggactgggt gaaaccaatg aagaaattat
    37861 tgaggtaatg cgcgacctgc gccgtcatgg tgtgacgatg ttaacgctgg ggcaatattt
    37921 gcagccaagc cgccatcacc tgccggttca acgttacgtt agcccggatg agttcgacga
    37981 aatgaaagcc gaagcgctgg cgatgggctt tacccatgct gcatgcggtc cgtttgtccg
    38041 ctcttcttac cacgccgatt tgcaggcgaa agggatggaa gttaagtaat cctgtaataa
    38101 ttacttacat ctgtctgata aaaaac
//
LOCUS       DAJVQG010000020        37983 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJVQG010000020 DAJVQG010000000
VERSION     DAJVQG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37983
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.020"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..1222
                     /locus_tag="OQM83_000949"
     CDS             <1..1222
                     /locus_tag="OQM83_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1519269.1"
                     /translation="LIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGS
                     VIAALSDSIWGIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAI
                     AMVLGPIITHKLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKV
                     LAEPRLLKLNFGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVP
                     FIIYAEVKRKMKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLP
                     SLISKESPAGYKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWL
                     AVASTMKEPPYVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSK
                     VTNRFEVEQAIRQA"
     gene            complement(1350..1841)
                     /gene="yajQ"
                     /locus_tag="OQM83_000950"
     CDS             complement(1350..1841)
                     /gene="yajQ"
                     /locus_tag="OQM83_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCS1519270.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2009..2920
                     /gene="panE"
                     /locus_tag="OQM83_000951"
     CDS             2009..2920
                     /gene="panE"
                     /locus_tag="OQM83_000951"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCS1519271.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            2883..3473
                     /gene="yajL"
                     /locus_tag="OQM83_000952"
     CDS             2883..3473
                     /gene="yajL"
                     /locus_tag="OQM83_000952"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCS1519272.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKTHEVASQLVMAAGIYNYYE"
     gene            complement(3527..4975)
                     /gene="thiI"
                     /locus_tag="OQM83_000953"
     CDS             complement(3527..4975)
                     /gene="thiI"
                     /locus_tag="OQM83_000953"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCS1519273.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5181..5423
                     /gene="xseB"
                     /locus_tag="OQM83_000954"
     CDS             5181..5423
                     /gene="xseB"
                     /locus_tag="OQM83_000954"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCS1519274.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDTSLPPFTPDNE"
     gene            5423..6322
                     /gene="ispA"
                     /locus_tag="OQM83_000955"
     CDS             5423..6322
                     /gene="ispA"
                     /locus_tag="OQM83_000955"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCS1519275.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6347..8209
                     /gene="dxs"
                     /locus_tag="OQM83_000956"
     CDS             6347..8209
                     /gene="dxs"
                     /locus_tag="OQM83_000956"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCS1519276.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8264..9238
                     /gene="yajO"
                     /locus_tag="OQM83_000957"
     CDS             8264..9238
                     /gene="yajO"
                     /locus_tag="OQM83_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCS1519277.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9292..9810)
                     /gene="pgpA"
                     /locus_tag="OQM83_000958"
     CDS             complement(9292..9810)
                     /gene="pgpA"
                     /locus_tag="OQM83_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCS1519278.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9788..10765)
                     /gene="thiL"
                     /locus_tag="OQM83_000959"
     CDS             complement(9788..10765)
                     /gene="thiL"
                     /locus_tag="OQM83_000959"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCS1519279.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(10843..11262)
                     /gene="nusB"
                     /locus_tag="OQM83_000960"
     CDS             complement(10843..11262)
                     /gene="nusB"
                     /locus_tag="OQM83_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCS1519280.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11282..11752)
                     /gene="ribE"
                     /locus_tag="OQM83_000961"
     CDS             complement(11282..11752)
                     /gene="ribE"
                     /locus_tag="OQM83_000961"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCS1519281.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(11841..12944)
                     /gene="ribD"
                     /locus_tag="OQM83_000962"
     CDS             complement(11841..12944)
                     /gene="ribD"
                     /locus_tag="OQM83_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCS1519282.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGGVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(12948..13397)
                     /gene="nrdR"
                     /locus_tag="OQM83_000963"
     CDS             complement(12948..13397)
                     /gene="nrdR"
                     /locus_tag="OQM83_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCS1519283.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13548..14087
                     /locus_tag="OQM83_000964"
     CDS             13548..14087
                     /locus_tag="OQM83_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCS1519284.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14386..15270
                     /gene="tsx"
                     /locus_tag="OQM83_000965"
     CDS             14386..15270
                     /gene="tsx"
                     /locus_tag="OQM83_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCS1519285.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15447..15794)
                     /locus_tag="OQM83_000966"
     CDS             complement(15447..15794)
                     /locus_tag="OQM83_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCS1519286.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(15923..16894)
                     /gene="secF"
                     /locus_tag="OQM83_000967"
     CDS             complement(15923..16894)
                     /gene="secF"
                     /locus_tag="OQM83_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCS1519287.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(16905..18752)
                     /gene="secD"
                     /locus_tag="OQM83_000968"
     CDS             complement(16905..18752)
                     /gene="secD"
                     /locus_tag="OQM83_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCS1519288.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18780..19112)
                     /gene="yajC"
                     /locus_tag="OQM83_000969"
     CDS             complement(18780..19112)
                     /gene="yajC"
                     /locus_tag="OQM83_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCS1519289.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19135..20262)
                     /gene="tgt"
                     /locus_tag="OQM83_000970"
     CDS             complement(19135..20262)
                     /gene="tgt"
                     /locus_tag="OQM83_000970"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCS1519290.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20318..21388)
                     /gene="queA"
                     /locus_tag="OQM83_000971"
     CDS             complement(20318..21388)
                     /gene="queA"
                     /locus_tag="OQM83_000971"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCS1519291.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21481..22062
                     /gene="acpH"
                     /locus_tag="OQM83_000972"
     CDS             21481..22062
                     /gene="acpH"
                     /locus_tag="OQM83_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HCS1519292.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22067..23884)
                     /gene="malZ"
                     /locus_tag="OQM83_000973"
     CDS             complement(22067..23884)
                     /gene="malZ"
                     /locus_tag="OQM83_000973"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCS1519293.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24040..25413)
                     /gene="proY"
                     /locus_tag="OQM83_000974"
     CDS             complement(24040..25413)
                     /gene="proY"
                     /locus_tag="OQM83_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCS1519294.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25489..26808)
                     /gene="brnQ"
                     /locus_tag="OQM83_000975"
     CDS             complement(25489..26808)
                     /gene="brnQ"
                     /locus_tag="OQM83_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCS1519295.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27215..28510)
                     /gene="phoR"
                     /locus_tag="OQM83_000976"
     CDS             complement(27215..28510)
                     /gene="phoR"
                     /locus_tag="OQM83_000976"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCS1519296.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28568..29257)
                     /gene="phoB"
                     /locus_tag="OQM83_000977"
     CDS             complement(28568..29257)
                     /gene="phoB"
                     /locus_tag="OQM83_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCS1519297.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29447..30649
                     /gene="sbcD"
                     /locus_tag="OQM83_000978"
     CDS             29447..30649
                     /gene="sbcD"
                     /locus_tag="OQM83_000978"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCS1519298.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETTQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30646..33789
                     /gene="sbcC"
                     /locus_tag="OQM83_000979"
     CDS             30646..33789
                     /gene="sbcC"
                     /locus_tag="OQM83_000979"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCS1519299.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            33915..35099
                     /gene="araJ"
                     /locus_tag="OQM83_000980"
     CDS             33915..35099
                     /gene="araJ"
                     /locus_tag="OQM83_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCS1519300.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35242..36150)
                     /gene="mak"
                     /locus_tag="OQM83_000981"
     CDS             complement(35242..36150)
                     /gene="mak"
                     /locus_tag="OQM83_000981"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCS1519301.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36275..37186
                     /gene="rdgC"
                     /locus_tag="OQM83_000982"
     CDS             36275..37186
                     /gene="rdgC"
                     /locus_tag="OQM83_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCS1519302.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37264..37719)
                     /locus_tag="OQM83_000983"
     CDS             complement(37264..37719)
                     /locus_tag="OQM83_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1519303.1"
                     /translation="MTQHPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37716..>37983)
                     /locus_tag="OQM83_000984"
     CDS             complement(37716..>37983)
                     /locus_tag="OQM83_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HCS1519304.1"
                     /translation="NDHLRQTALRNAHTPASLLTTLTESQDRSLAINNPQLAADVKTA
                     WLKEDLSLLLFVEQPDLSQLRDLVKTGATRKIRSEARHRLEEKQ"
BASE COUNT         9378 a        10236 c         9643 g         8726 t
ORIGIN      
        1 attaatcggt attgccattg gtatttatgg tctgactcag gccgtttttc agattccgtt
       61 tggcctgctt tccgaccgca ttggtcgcaa accattaatt gtcggtgggc tggcggtgtt
      121 tgccgccggt agcgttatcg ctgcgctttc cgactccatc tggggaatta ttctgggccg
      181 ggcgctacaa ggctctggag caattgccgc cgccgttatg gcgctgcttt ccgatctcac
      241 gcgcgaacaa aaccgcacca aagcgatggc gtttatcggc gtgagctttg gcattacctt
      301 tgccattgcg atggtgcttg gcccgatcat cactcacaaa cttgggctgc acgcgctgtt
      361 ctggatgatc gctattctgg caacaaccgg cattgcgttg accatttggg ttgtgcccaa
      421 cagtagcact cacgtactta atcgtgagtc cggaatggtg aaaggcagtt tcagtaaagt
      481 gctggcggaa ccgcggctgc tgaaactcaa ctttggcatt atgtgtctgc acatgcttct
      541 gatgtctacg tttgttgccc tgcccggaca gctagctgat gcggggttcc cggcggctga
      601 acactggaag gtctatctgg cgacgatgtt aatcgccttt ggctcgatcg tgcctttcat
      661 tatctacgct gaagttaagc gcaaaatgaa gcaagtcttt gtcttctgcg tcgggttgat
      721 cgtggttgcg gaaattgtgt tgtggaacgc acaaacgcag ttctggcaac tggtggtcgg
      781 cgtgcagctt ttctttgtgg cgtttaattt gatggaagcc cttctgccct cacttatcag
      841 taaagagtcg ccagcaggtt acaaaggtac ggcgatgggt gtctactcca ccagccagtt
      901 tcttggcgtg gcgattggcg gttcgctggg cggctggatt gacggcatgt ttgacggtca
      961 gggggtattt ctcgctggcg caatgctggc cgcagtgtgg ctggcagtcg ccagtaccat
     1021 gaaagaaccg ccgtatgtca gcagtttgcg cattgaaatc ccggcggaca ttgccgcaaa
     1081 cgaagcgtta aaagtgcgtt tgctggaaac tgaaggcgtc aaagaagtgt tgattgcaga
     1141 agaagagcat tcagcttatg tgaaaatcga cagcaaagtg acaaatcgct ttgaggttga
     1201 acaggcaatt cgtcaggcat aaaaaacgcg gcgaattgcc tgctgcgcta cgcttaccag
     1261 gcctacaaag ttcttgagtt acaaaccgtt gtaggccgaa tcaggcgttc atgccgcatc
     1321 tgacatgaac aaaacgcaca ttgtcgcgat taatcgcgga agtttttgaa ctggaacggc
     1381 tggccgagat cgccaccacg taccatcgcc atgacagcct gcaaatcatc acgagatttg
     1441 ccagtcacac ggatctcatc gccctgaatt tgcgcctgca ctttcagttt gctgtcttta
     1501 atcatcttga cgattttctt ctgggtcgcg ctctcaatgc cctgtttcag tttcgcttcc
     1561 acaaaccagg ttttaccgct atgaacgata ttttccggta catccagtga actgccttca
     1621 atgccgcgct tcagcagctt ggcacgcaga atatccagca actgattgac ctggaaatcg
     1681 gactcgctca acactttgat ggttttgctg gcgtcgttca gctcaaatga ggcttcaaca
     1741 ttacggaagt caaaacggga ctccacttcg cggctcgcgt tatcgaccgc gttacgtgct
     1801 tcctgaagat caacttcaga gacaatatcg aaagatggca tcttttcctc tcccttcatt
     1861 tttgatgcga agcataatac ccgcaaagtt aaataaccga cagtcctgca ccaggacgta
     1921 tcccttaatt gtgcatagtc acaacagcat gcacaattaa gggatagcct tattgaacac
     1981 ccggagtggt tgcgggtgag gaggaacaat gaaaattacc gtattgggat gcggtgcctt
     2041 agggcaatta tggcttacag cactttgtaa acagggtcac gaagttcagg gctggctgcg
     2101 cgtaccgcaa ccttattgta gtgtgaatct ggttgagact gatggttcga tatttaacga
     2161 atcgctgacc gccaacgatc ccgattttct tgccaccagc gatctgctcc tggtgacgct
     2221 gaaagcatgg caggtttccg atgccgtcaa aagcctcgcg tccacactgc ctgtaaccac
     2281 gccaatactg ttaattcaca acggcatggg caccatcgaa gagttgcaaa acattcagca
     2341 gccattactg atgggcacca ccacccatgc cgcccgccgc gacggcaatg tcattattca
     2401 tgtggcaaac ggaatcacgc atattggtcc ggcacggcaa caggacggcg attacagcta
     2461 tctggcggat attttgcaaa ccgtgttgcc tgacgttgcc tggcataaca atattcgcgc
     2521 cgagctgtgg cgcaagctgg ccgtcaactg tgtgattaat ccactgaccg ccatctggaa
     2581 ttgcccgaat ggtgaattac gtcatcatcc gcaagaaatt atgcagatat gcgaagaagt
     2641 cgcggcagtg atcgagcgcg aagggcatca tacttcagca gaagatttgc gtgattacgt
     2701 gatgcaggtg attgatgcca cagcggaaaa tatctcgtcg atgttgcagg atatccgcgc
     2761 gctgcgccac accgaaatcg actatatcaa tggttttctc ttacgccgcg cccgcgcgca
     2821 tgggattgcc gtaccggaaa acacccgcct gtttgaaatg gtaaaaagaa aggagagtga
     2881 atatgagcgc atcggcactg gtttgcctcg cccctggtag tgaagagact gaagccgtca
     2941 ccactatcga tctgctggtt cgcggcggta tcaaagtcac cactgccagc gtcgccagcg
     3001 atggcaacct ggccattacc tgctcgcgcg gcgtgaagct gctggcggat gcgccgctgg
     3061 tcgaggtggc tgatggcgaa tatgacgtga tcgtactgcc tggtggcatt aaaggcgcag
     3121 agtgttttcg cgatagtacc ctgctggttg aaaccgttaa acagttccac cgttccgggc
     3181 gtatcgtcgc ggctatttgc gccgcgccag ccaccgtgct ggtaccgcac gatatcttcc
     3241 cgattggcaa tatgaccggc ttcccgacgc tgaaagacaa aattcccgcc gaacaatggc
     3301 aggacaagcg cgtcgtctgg gatgcacggg taaaattgct gaccagccag gggccgggta
     3361 cggctattga ttttggtctg aaaattatcg acctgctggt tgggcgcgag aaaacccatg
     3421 aagtggcatc acaactggtg atggcggcag ggatttataa ttattacgag tagtgtcgga
     3481 tgcggcaaac gtcgcatctg accagatgcg acgtaaaaac cacaaattat gggcgataca
     3541 ccttcacatt gttaaagccc tgctcgcgca gatagagcgc ctgcagacgg ctcatcaccc
     3601 cgcgctcaca ccacagtagc caggttctgt tctggtcgag atcgccaaat ttggtgctca
     3661 gtttatagaa cggcagagaa accacgtcga taccttcaac cttcagcggc ttatcttcct
     3721 gctcatcgac agaacggata tcgaggatca cgtcgttcgg gccaaagcca ttgacggttt
     3781 ccacttccac cacttcctgc tcggtctgct gggcgatttc gcggatatca acgttattcg
     3841 cttcctcaac cactttatcg agaatactga agtcgaactt ctcttcttcc gcttcaatct
     3901 tcgatttaac cgctttcacc gtcgggcttt tggagatcac gccacaatat tctggcatcg
     3961 tgcgagcaaa gtcttcagtg ccaatctggc gggccaggtt gatgatgtgc tctttgtcgt
     4021 aagaaatcag cggacgcagg atcagcgtat cagagacgtt atcaatcagg cgcaggttgg
     4081 tcagcgtctg actggacacc tggccgagcg cttcgccggt gaccagcgcc tgtacgccgt
     4141 aacgttcagc cactttagac gcggcacgca ccatcatacg tttgaggata acgcccatct
     4201 gaccgtcgtc gattttctcg agaatttccc cgacgaccgg ttcgaaatta atggcgacaa
     4261 aacgcacgcg gtgggagctg ccaaagcggt tccacagata atgcgccacc tgacgcacgc
     4321 caatttcatg cgctgcgccg ccgaggttaa agaagcagta atgcacgcgg cagccgcgac
     4381 gcatcaacat ataactggaa acaccggagt cgaaaccacc ggaaatgagc gacagcacat
     4441 cttcctgggt gccaatcggg aaaccgccaa taccttcgta gcggccttta atcagcagaa
     4501 gacgatcgtc ttccacttcc agatggacag ttacatccgg gttagtcagc ttcacgcgcg
     4561 cggattcaat atgctgattt aaaccgccgc cgacgtaacg ttccacatca atcgagctaa
     4621 aatcatgttt gccacggcgc ttcacgcgta cgcagaaggt tttgccttcc agctgatcgc
     4681 gatattgaac caacgctttc tcgaaaatat cgtgcatgtc ggtaaacggc acgtcttcga
     4741 cttcgagaat atggtggata cccggaatac gggtcagggc gtcgcgaata gccagacgct
     4801 gtttttcatc ttttgcgcga acttcgatgt tatcccagtg gcggacgaca gcgagcgtct
     4861 catcatagtg ctttaaaacg ttacgaatgt tcccggtaag gatttttata aagcgcaagc
     4921 gcacagattg gcttttgatg gtgatttccg ggaacaattt aatgataaac ttcatggcgg
     4981 caatggttcg ttggcaagcc ttaagcgact tgtataggga aaaatacagc agcccacacc
     5041 tgcggctgca tccaggcgcg gaagtatacc actaacatcg ctttgctgcg cacatcacct
     5101 taccattgcg cgttatttgc tatttgccct gagtccgtta ccatgacggg gcgaaaaata
     5161 ttgagagtca gacattcatt atgccgaaga aaaatgaggc gcccgccagc tttgaaaagg
     5221 cgctgagcga gctggaacag attgtaaccc gtctggaaag tggcgacctg ccgctggaag
     5281 aggcgctgaa cgagttcgaa cgcggcgtgc agctggcacg tcaggggcag gccaaattac
     5341 aacaagccga acagcgcgta caaattctgc tgtctgacaa tgaagacacc tctctacccc
     5401 cttttacacc ggacaatgag taatggactt tccgcagcaa cttgaagcct gcgttaagca
     5461 ggccaaccag gcgctgagcc gttttatcgc cccactgccc tttcagaaca ctcccgtggt
     5521 cgaaaccatg cagtatggcg cattattagg tggtaagcgc ctgcgacctt ttctggttta
     5581 tgccaccggt catatgttcg gcgttagcac aaacacgctg gacgcacccg ctgccgccgt
     5641 tgagtgtatc cacgcttact cattaattca tgatgattta ccggcgatgg atgatgacga
     5701 tctgcgtcgc ggtttgccga cctgccatgt gaagtttggc gaagcaaacg cgattctcgc
     5761 tggcgacgct ttacaaacgc tggcgttctc gattttaagc gatgccgata tgccggaagt
     5821 gtcggaccgc gacagaattt cgatgatttc agaactggca agcgccagcg gtattgccgg
     5881 aatgtgcggt ggtcaggcat tagatttaga tgcggaaggc aaacacgtac ctctggacgc
     5941 actcgagcgt attcatcgtc ataaaaccgg cgcattgatt cgcgccgccg ttcgccttgg
     6001 tgcattaagc gccggagata aagggcgtcg tgctctgcca gtactcgaca agtacgcaga
     6061 gagcatcggc cttgccttcc aggttcagga tgacatcctg gatgtggtgg gagatactgc
     6121 aacgttggga aaacgccagg gtgccgacca gcaacttggt aaaagtacct accctgcact
     6181 tctgggtctt gagcaagccc ggaagaaagc ccgggatctg atcgacgatg cccgccagtc
     6241 gctgaaacaa ctggctgaac aatcactcga tacctcggca ctggaagcgc tagcggacta
     6301 catcatccag cgtaataaat aaacaataag tattaatagg cccctgatga gttttgatat
     6361 tgccaaatac ccgaccctgg cactggtcga ctccacccag gagttacgac tgttgccgaa
     6421 agagagttta ccgaaactct gcgacgaact gcgccgctat ttactcgaca gcgtgagccg
     6481 ttccagcggg cacttcgcct ccgggctggg cacggtcgaa ctgaccgtgg cgctgcacta
     6541 tgtctacaac accccgtttg accaattgat ttgggatgtg gggcatcagg cttatccgca
     6601 taaaattttg accggacgcc gcgacaaaat cggcaccatc cgtcagaaag gcggtctgca
     6661 tccgttcccg tggcgcggcg aaagcgaata tgacgtatta agcgtcgggc attcatcaac
     6721 ctccatcagt gccggaattg gtattgcggt tactgccgag aaagaaggca aaaatcgccg
     6781 caccgtctgt gtcattggcg atggcgcgat taccgctggc atggcgtttg aagcgatgaa
     6841 tcacgcgggc gatatccgtc ctgatatgct ggtggtcctc aacgacaatg aaatgtcgat
     6901 ttccgaaaat gtcggcgcgc tcaataacca tctggcacag ctgctttccg gtaagcttta
     6961 ctcttcgctg cgcgaaggcg ggaaaaaagt tttctctggc gttccgccaa ttaaagagct
     7021 gctcaaacgt accgaagaac atattaaagg catggtagtg cctggcacgt tgtttgaaga
     7081 gctgggcttt aactacatcg gcccggttga cggtcacgat gtgctggggc ttatcaccac
     7141 gctgaagaac atgcgcgacc tgaaaggccc gcagttcctg catatcatga ccaaaaaagg
     7201 tcgtggttat gaaccggcag aaaaagaccc catcactttc cacgccgtgc ctaaatttga
     7261 tccctccagc ggttgtttgc cgaaaagtag cggcggtttg ccgagctatt caaaaatctt
     7321 tggcgactgg ttgtgcgaaa cggcagcgaa agacaacaag ctgatggcga ttactccggc
     7381 gatgcgtgaa ggttccggca tggtcgagtt ttcacgtaaa ttcccggatc gttacttcga
     7441 cgtggcaatc gccgagcaac acgcggtgac ctttgccgcc ggtctggcga ttggtgggta
     7501 caaacccatt gtcgcgattt actccacttt cctgcaacgc gcctatgatc aggtgctgca
     7561 tgacgtggcg attcaaaagc tcccggtcct gttcgccatc gaccgcgcgg gcattgttgg
     7621 tgctgacggt caaacccatc agggcgcttt tgacctctct tacctgcgct gcataccgga
     7681 aatggtcatt atgaccccga gcgatgaaaa cgaatgtcgc cagatgctct ataccggcta
     7741 tcactataac gacggcccgt ccgcggtgcg ctacccgcgc ggtaacgcgg ttggcgtgga
     7801 actgacgccg ctggaaaaac tgccaattgg caaaggcatt gtgaagcgtc gtggcgagaa
     7861 actggcgatc cttaactttg gtacgctgat gccagacgcg gcgaaagtcg ctgaatcgct
     7921 gaacgctacg ctggtcgata tgcgttttgt gaaaccgctt gatgaagcgt taattctgga
     7981 aatggccgcc agccatgaag cgctggtcac cgtagaagaa aacgccatta tgggcggcgc
     8041 aggcagcggc gtgaacgaag tgctaatggc ccatcgtaaa ccagtacccg tgctgaacat
     8101 tggcctgcct gacttcttta ttccacaagg aactcaggaa gaaatgcgcg ccgaactcgg
     8161 cctcgatgcc gccggtatgg aagccaaaat caaggcctgg ctggcataat ccctactcca
     8221 ctcctgctat gcttaagaaa ttattcatgg caggagtggc agcatgcaat acaacccctt
     8281 aggaaaaacc gaccttcgcg tttcccgact ttgcctcggc tgtatgacct ttggcgagcc
     8341 agatcgtggt aatcacgcat ggacactacc ggaagagagt agccgtccca tcattaaacg
     8401 cgcgctggaa ggcggcataa atttctttga taccgccaat agctattccg atggcagcag
     8461 cgaagagatc gttggtcgcg cactgcggga tttcgcccgt cgtgaagacg tggtcgttgc
     8521 gacgaaagtg ttccatcgcg ttggtgattt accggaagga ttatcccgtg cacaaatttt
     8581 gcgctctatc aacgacagcc tgcgccgtct cggcatggat tatgtcgata tcctgcaaat
     8641 tcatcgctgg gattacaaca cgccgatcga agagacgctg gaagccctga acgacgtggt
     8701 aaaagccggg aaagcgcgtt atatcggcgc gtcatccatg cacgcttcgc agtttgctca
     8761 ggccctgaag ctacaaaaac agcacggctg ggcgcagttt atcagtatgc aggatcacta
     8821 caatctgatt tatcgcgaag aagagcgcga gatgctgcct ctgtgttatc aggagggcgt
     8881 ggcggtgatt ccgtggagcc cgctggcgcg ggggcgactg acgcgtccgt ggggagaaac
     8941 taccgcacga ctggtgtctg atgaggtggg gaaaaatctc tataaagaaa gcgatgaaaa
     9001 tgacgcgcag atcgcagagc ggttaacggg cgtcagtgaa gaactcggtg caacacgagc
     9061 acaagttgcg ctggcctggt tattgagtaa accgggcatt gccgcaccga ttatcggtac
     9121 atcgcgggaa gaacagcttg atgagctgct gaacgcggtg gatatcactt tgaagccaga
     9181 acagattgcc gaactggaaa cgccgtataa accgcatgcg gtcgtaggat ttaaataaga
     9241 gtccaggcct gatgagacgt gacaagcgtc acatcaggca tcggtgcaca actacgacag
     9301 aatacccagc ggccagtgat gaccgataaa gtacaggatg cctgcggaaa tcaccccggc
     9361 gacaatatcg tcgatcatga tccccatgcc gccatgtaca ttgcgatcaa accagcggat
     9421 cggccacggc ttccacatat ccagaatacg gaaaatcaca aacccggcgg caacccactg
     9481 ccagtcattg gtcggcagcg ccatgagcgt gatccacata ccaataaatt cgtcccagac
     9541 aatgctgccg tgatcgtgca cgcccatatc tttcgccgtt tgatgacaaa gatagacgcc
     9601 gatacagatc cccaacatca ccaccagcga gtagagctgc cagggtaaaa aggtcatcag
     9661 ataccagaac ggaatcgctg ccagcgatcc catcgtgcca ggaacgattg ggcttaatcc
     9721 actcccgaat ccgacagcaa gcagatgcca cggattactc atcttcaggc gacttttcgc
     9781 gacatcttta tggcgtggca aaatggtcat atcctttcca gtcaaacgtg acaggttcgc
     9841 cgtcacgaat aaaacaaagc ccttcgatat cggcggtcat ttgcccgata caggtaaacg
     9901 gtacgccaag gtgtccgaga gcaacatcca gcgcgccacg gttcagttcc ggcaccgtga
     9961 aacacaactc gtaatcttca ccgccagaga gcgcccagcg cagcgcctgt tccggttcaa
    10021 catggcgaga aagcgcatca gaaaacggca gcaatgccag gtcaatacgt gcgccgcagt
    10081 cgctggcttt cacgatatgc ccgagatcgg aaatcagacc gtcagagaga tcgatagctg
    10141 aatttgccag atcgcgcagt gcctgcccct gtaaaatacg cggcgatgga cggagatgac
    10201 gtttgatcaa gtagtcagca tctttagcat cggcaacctg caaacggttt tgcaaaatcg
    10261 ccagcccggc ggcgctatcg cccggtgtac cggtcacata gatccagtca cccggtttcg
    10321 ccccagagcg cgttaaggct cgtcccatcg gaacaaagcc gtggataccc aacgtcattg
    10381 ataatggccc acgcgtggta tcgccgccaa tgagttgcat atcgtaataa ttgagaagat
    10441 caaacaaact gtcgctgaag gactcaagcc acgcttcgtc tacgtccggt aaggttaatg
    10501 ccagcgtcag ccaggccgga tcggccccca tcgctgccag atcgcttagg ttcaccgcca
    10561 gtgctttata agccagatca gcaggatcga tatcagggag gaaatggtta cccgccacca
    10621 gcgtatcagt gctgatcgcc agggtctgtt tctcggggat attgagaagt gcgcaatcgt
    10681 cgccgatgcc cagttcgaca tcaagacgag aacttcttac acggtcaaaa taacgggcaa
    10741 tcagggagaa ctcgccacat gccatacgtt atgcctcagc aggtaaaaaa gaaaaggccg
    10801 acgatcatgg aacggtcttc cgtgaatcta ccggcctgga tatcactttt tgttagggcg
    10861 aatcacaggt gctgctttat cgagtacgcc gttgacgaac ttatggctgt cttctgcgcc
    10921 gaacgatttc gccagttcga tcgcttcgtt aatggccact ttgtatggca catcgctacg
    10981 tttagacagt tcgtacagcg caatgcgcag tactgctttt tctacctgtc ccagttcttc
    11041 cagcaggcgg gacaggtatg gcttcatcag tccgtcgagg tatgcggtat tagtcgccac
    11101 cccggccagc agctcacgga agtacaggac gtcaacgtct tttacatcct gttcagccag
    11161 gaactggtat tcaacatcag cgatgtcgtt ctgggacaac tgccaggagt agagcgcctg
    11221 gacggcacac tcacgagcgc ggcgacgagc agcaggtttc acggatttcc ccttactaat
    11281 ttcaggcctt gatggctttc aatacattaa tcatttcaag cgcggtcagt gcagcttctg
    11341 cacctttgtt gccagctttg gtgccagcac gttcgatcgc ttgttcaatg ctttcagtgg
    11401 tcagaacccc aaaagcaacc ggaatttcgc tgtcctgggc aacatgcgcc aggccgttgc
    11461 ttgcaccacc agcgacatat tcaaagtggg cagtgccacc acgaataacc gtacccagcg
    11521 caatcaccgc gtcgtattta ccggttttag ccagtgcacc cgccgccagc ggcagctcat
    11581 aggcaccagg cacccaaaca acggtaatgt tttcatcttt tacctgaccg atacgtttca
    11641 gtgcgtcaat tgcaccttcc agcaggctgt cattgataaa gttgttgaaa cgcgcgatgg
    11701 tgatggcgac gcgagcgtcc ggggtagcaa cgttagcttc aataatgttc attttcttcc
    11761 ttcgggttcg agtatggccc cgcagggggg cggattttag cataatattt cgtgcgctgc
    11821 ttccctttcg agccgggaga tcatgcaccc actaaatgca ggcaaacatc cgggcctaca
    11881 tgacgtatct ctttgaattt aaattggggg gcgtcggcta atttctcaag ccctggcagc
    11941 gtgcataatc cgcgggcgtc gctgcctaat agtttaggtg cgatatagac aatcagctca
    12001 tcgactaaac ccgcctgcag caatgcgcca gcgagcgttg gccccgcttc cacccagatg
    12061 ctgttaattt gctgtttacc cagttgcatc atcagtacaa ccagatccag atgaccttta
    12121 tgctctggaa tcagcaaggt acgcaccgtt tccggccact cactagaatc ttcctgcgta
    12181 cgcgcgaacc aggtttcgcc gggctgctgc acaatgcgat gttccggcgt cacgcgattt
    12241 tggctatcaa tcacaatacg tactggctgg cggagatttt gttgcgggta gagcgcctga
    12301 gtttgttcat ccagttcaga ccaacgcact gttaaggctg gatcatccgc cagcaccgtg
    12361 gcgctgctgg ttaaaatggc atgactttgt gcgcgctgtc gttgtacgtc gcgccgcgcc
    12421 tgaggcgaag tgatccactg gctttcgccg ctcgccatcg ccgtgcggcc atcaagcgat
    12481 gcgccaagtt ttaactgaat ataaggaaag ccggtgcgca tccgcttgag aaagccttta
    12541 ttcaattgct cggcttcact catcatcagc ccgtggctga cgtcaatgcc agcctgttgc
    12601 agacggtaaa gtccacgccc agcgacctgc gggttaggat cctgcatcgc tgcgaccacg
    12661 cgcgctacgc cagcggcgat gagtgcgtca cagcacggtg gcgtacggcc atgatggcta
    12721 cagggttcga gtgtcacata cgccgttgcg cctttggctt tttcacccgc catacgcaac
    12781 gcgtgtactt cggcatgtgg ttcacccgct cgttggtggt aaccttcacc gacaatttcg
    12841 ccatctttga caatgacgcc cccgacattc gggttggggt gggtggtaaa acgtcctcgt
    12901 tgcgccagct ttagcgcccg cgccatgtaa tactcgtcct gcacggctta gtcctccagg
    12961 cgcgcgatct cttcgccaaa ttctttgata tcttcgaaac tgcggtagac agaggcaaaa
    13021 cggatatagg cgactttatc gagctttttc aattgctcca tcaccagatt gccaatcatc
    13081 ttgctcggta cttcgcgctc accggtggcg cgcagctgcg atttaatatg attgattgcc
    13141 atttcaacgt catcggaact caccggacgt ttttccagcg cccgcagcat tccgctacgc
    13201 aatttctctt cattaaacgg ttcacgcacg tcgttgcttt ttacaacacg cggcataacc
    13261 agctccgcca cttcaaaggt ggtgaaacgt tcattacaca ccagacactg ccgacggcgg
    13321 cgtacggatg aaccctcgcc cacgagacga gagtcaatta ccttagtgtc cacggcgaaa
    13381 cagaatgggc aatgcatacg gcgtcctgac caggtggtta acggaaatct attttaccct
    13441 gaactgacga tacaacaaag gcgcaacgct tttgagataa aggatcgatg ctttcgctgc
    13501 ttttaccctc taccattagg ctaattgcga cgagaaaggg acgacctatg aatactaacg
    13561 tttttcgact gctcctgctg ggaagcctgt tcagccttag cgcctgtgtg cagcaaagtg
    13621 aagtgcgaca gatgaaacac agcgtcagca cgctgaacca ggagatgacg caactcaatc
    13681 aagaaacagt caaaatcact cagcaaaaca ggctgaatgc aaaatccagc agtggggttt
    13741 accttctgcc tggagcgaaa acaccggcaa gactggaaag ccagatcggt actttacgta
    13801 tgtcgctggt gaatattacg cctgatacag atggcaccac ccttacacta cgtattcagg
    13861 gtgagtcgaa tgatcccttg cccgcattca gcggaactgt tgaatatggg caaattcagg
    13921 gaacaataga caactttcag gaaatcaatg tgcagaatca attgattaat gcccctgcca
    13981 gcgtcctcgc ccccagcgat gttgatattc cgttacaatt aaaggggatt tctgtggatc
    14041 agttaggctt tgtacgtatc cacgacattc aacctgttat gcagtaaacg tatttcggga
    14101 cgattttgtg cgtcccgcaa catctttccc cgtcattttg ttactctgct tacatcacct
    14161 ggattgatag taaaagtttg caacaagggc gaaagtcagt acaatccccg cccgaatgtg
    14221 tgtaaacgtg aacgcaatcg attacgtaaa tgatagaact gtgaaacgaa acatattttt
    14281 gtgagcaatg atttttataa taggctcctc tgtatacgaa atatttagaa acgcaatttg
    14341 cgcctttttc actcccgcaa gggattttca aacagtggca tacatatgaa aaaaacatta
    14401 ctggcagccg gtgcggtact ggcgctctct tcgtctttta ctgtcaacgc agctgaaaac
    14461 gacaaaccgc agtatctttc cgactggtgg caccagagcg ttaacgttgt cggaagctat
    14521 cacacccgtt tcggaccgca gatccgcaac gatacctacc ttgagtacga agcattcgct
    14581 aaaaaagact ggttcgactt ctatggttat gcggatgcgc cggtattctt cggcggtaac
    14641 tccgatgcta aaggtatctg gaaccacggt tctccgctgt ttatggaaat cgaaccacgt
    14701 ttctccatcg acaagctgac caatactgac cttagcttcg gtccgttcaa agagtggtac
    14761 ttcgcgaaca actacattta cgacatgggt cgtaataaag atggtcgcca gagcacctgg
    14821 tacatgggtc tgggtaccga catcgatact ggtctgccga tgagcctgtc catgaacgtc
    14881 tatgcgaaat accagtggca gaactatgac gcagcgaacg aaaacgagtg ggacggttac
    14941 cgtttcaaaa ttaaatactt tgtgccgatt accgatctgt ggggcggtca gctgagctac
    15001 atcggcttca ccaacttcga ctggggttcc gatttagggg atgacagcgg taacgcaatc
    15061 aacggtatta agacccgtac taataactct atcgcttcca gccatattct ggctctgaac
    15121 tacgatcact ggcactactc tgtcgtagct cgttactggc acgacggtgg tcagtggaac
    15181 gacgatgcag aactgaactt cggcaacggc aacttcaacg ttcgctctac cggctggggt
    15241 ggttacctgg tagtaggtta caacttctga ttatgaaaat gccgggattt attcccggca
    15301 tttctgattg ttaacaacgt gtgctttgtt catgccggat gcggcgtaaa cgctttgtcc
    15361 ggcccacaaa agcacgtaaa ttcaatatat tgctgaaata acgtaggcct gataagcgta
    15421 gcgcatcagg caattttacg ttttaatcac ttcttcttgt tcatcatcgc tttcaggtca
    15481 gcgaatgggt tgtacttcgc ttcaccgaca tctttctgcg catcttcccc tgcgataacg
    15541 gtcgtaccat actgatccgc ttcggtatat ttcgaatgct catgatcgtg gcaatagaga
    15601 caaagcaatt cccagttact gccatcttcc gggttattgg tatggtcgtg atcaatgtgg
    15661 tgaacggtaa gttcacgcag gttggaataa acaaactcgc gggaacagcg accgcagacc
    15721 cacggataga tttttaatgc tttttcgcga tagccgcttt ctaaccgcgc atagtttttt
    15781 gggatgatag ccatgaaaag ttacctggat gaaatgtagg gttacgcgca gtttcccaag
    15841 atgcggggat tttcgcaatc tccatacatc aaaaaaaatc ccgatcttct gaccgggatt
    15901 ttcaacatca acgggaactt gattacggca gaattgacgg ctgatccgcc ccttcttttt
    15961 ccactttctg ctgcaacatg tgttcgcgct tcatacccag tttcagagcc aacgcagatg
    16021 ctacgtagat ggaagatgca gtgccgatgg aaacaccgat aagcatggtc agcgagaagc
    16081 cttccagtac cggaccaccg aagagataca gcatcaggat aaccatcaag gtagtaccgg
    16141 atgtgatcaa ggtacggtgc agcgtctggg tcaaggacac gttaaagatt tcgtaaggcg
    16201 taccgcgacg gatcttgcgg aagttttcac gaattcggtc cgataccacg atactgtcgt
    16261 taagcgagta accgataacc gacatcaacg atgccacaat ggtcaggtca atctcgatat
    16321 ggaataacga caaaataccc agcgtaatga taacgtcgtg cgccagcgca ataaccaccc
    16381 ctgccgccag tcgccactca aagcggaaac ctacgtacac gaggatagac agcagcgctg
    16441 ccatcaacgc catcgcaccg gtttgcgcaa ggtctgcccc cacgctcgga ccgacgaact
    16501 caatacgctt cactgctgca ttctgattgg tggattcgtt aatcaccttc agaacctggc
    16561 tgcccagcac ctgaccgccg gtttcgcctt cagcaggcgg catacggacc atgatgtcgt
    16621 ggctgctacc aaagttttgc agcattggct cttcaaaacc ggctttttgc aatgcatcac
    16681 gcattacgtc aatttcagcc ggtttttcga gcgtaatttc gataaccgta ccaccggtga
    16741 aatccagccc ccagttaaag ccgcgcacgc ccataataac gatagcagcg attaacagca
    16801 gaccagagat gccgaaagcc cagtagtccc agcgcataaa gtcatagact ttacggccgt
    16861 ggtttagttg ttcaacagta tattcctgtg ccacatcgca ctcctcagat tgacagcttc
    16921 ttgacgcgct tgccgccata tagcaggttt acgatggcac gcgtaccgac aatcgcggta
    16981 aacatcgacg tcgccacacc gataccggta gtaatcgcga accctttaat tgccccggta
    17041 cccactgcgt acaggatgat gactttaatc agcgtggtga tgttcgcatc gaagatagaa
    17101 ctgaatgcgc cacgataacc ttcatcaatt gcctgttgaa cagtacgtcc gttgctcaac
    17161 tcttctttaa tacgttcgtt gatcagtacg ttcgcatcga ccgctaccgc aagggttaag
    17221 acgatacccg cgatacctgg catactcagt gttgcgcctg gcaacagcga cataataccg
    17281 acgatcaaga tcaagttggc aatcagagca ctggtcgcaa tcagaccaaa cttcttatag
    17341 aagatgatca tgaacagaat agaaaccagc agaccggcaa ggcaagcctc cagcccctgc
    17401 tcaatgttct gcatacccag ggtcgggcca attgtacgtt cttcaacaat ctgaatcggc
    17461 gcgatcaacg caccggcacg cagcagcagt gacagctgac gggcttcgtt cgggttgttg
    17521 atgccggtga tacggaagct gttacccaga cgagactgga tgttggcgat gttaatcacc
    17581 tcttcctgtt tcaccagaac cgcacgacca tttgcatctt tcttaccgct gtctttgtac
    17641 tccacaaaca gggttgccat cggtttgccg atgttgtcct tggtgaagtt agacataatg
    17701 ttgccaccag cgctatcgag cgagatgtta acctgcggct ggttgtattc gtcctggctg
    17761 gaagtggagt cggtgatatg gtcaccggtc agaattacgc gtttgtacag cacaaccggc
    17821 tgaccttcgc gggtctgttt cacttcagag tcgcccggta cgcgaccgga tgccgcagcg
    17881 gcctgatcaa cattggtgtt taccagacgg aattccagcg ttgccgtcgc gcccagaatc
    17941 tctttcgcac gcgcagtgtc ctgaatacca ggcagttcaa caacgatacg gtcagcaccc
    18001 tgacgctgaa ccaccggctc cgccacgcca agttggttta cacggttacg caggatatta
    18061 atgttctgct gcaccgcata ttcacgcgct tcactcagac gagcatcgct cattaccgca
    18121 cgcagctggt tgctgccctg gctgctaatc accaggtccg gatggcgctt gctcagatac
    18181 gcaatggctt catcacgagc tttagcatcg cggaaagtga tgctcaggcc gtagttgttt
    18241 tctttacgaa cagtggtata cgggatgccc ttttcgcgca ggtcactgcg taggctatcg
    18301 atattttgtt cctggagttt gccaagcgcg gtgtccatat ccacttccat caggaagtga
    18361 acgccgccac gcaggtcaag gccgagcttc atcggctcag catgaatagc tgccagccag
    18421 cgcggcgttg ccggggcaag gttaagcgcc acgacgtatt tgtcacccat aacgcccatt
    18481 aatgcttcac gagcgcgcaa ctgggtgtca gtggagtcga agcgcgcaag aatagcgccc
    18541 tcttccagtg ccacagactt agcagttatt ttttcttctt gtaacgtttt ctggacctgg
    18601 atcagcgttt gctcactggc ggcgactccg cgcgcaccag tgatctgaac agccggatcc
    18661 tcaccaaaca ggttgggaag cgcatacagc agaccgatga caatcaccac gatcagcatg
    18721 acgtacttcc acaaaggata acggtttaac acggcaattc ccttagggaa aaattttaat
    18781 tacagcgcct tcatggtgcc tttcggcagg acggcagcta cgaagtcacg tttaataact
    18841 acttcagtgg tgtcattcag cgcgatagca atgtagccgt tttccgctac tttggttacg
    18901 cgaccaacca ggccaccgtt cgtcagaact tcatcacctt tggcaatgga gtccatcagc
    18961 tttttgtgtt ctttggtgcg cttctgctgt ggacgcagga tcatgaaata gaaaatcaga
    19021 ccgaatacca ccagcatcaa aatcaaagac atcgggctac cttgcgccgg tgcacccgtt
    19081 gccgctaccg catcagaaat aaaaaagctc attaaatttc cctcattatt aatattaatc
    19141 aacgttcaaa ggtggtactt ctcgcccctg acgctggtaa aaatcagtta cgaagctctc
    19201 taatttaccc tcttcaatag ccttgcgtaa acccgccatc aaacgctggt agtaacgaag
    19261 gttatgaatg gtgttgagtc gcgcgcctaa tatttcgttg caacggtcaa gatgatgcaa
    19321 gtaagcgcgt gaataattgc gacaggtgta gcaatcacac tcaggatcaa gtgggccagt
    19381 atcgctctta tacttcgcat tgcggatttt caccacgcca tcggtcacga acaaatgacc
    19441 atttcgggcg ttgcgggttg gcattacgca gtcaaacata tcgataccac gacgtacgcc
    19501 ttcaaccagg tcttctggtt taccaacgcc catcaggtaa cgcggtttgt ctgccggaat
    19561 ttgcgggcat acatgctcca gaatgcggtg catatctgct ttcggctcac ccacagccag
    19621 accgccgaca gcgtagccat caaaaccgat atctaccaga cctttaacag aaatatcacg
    19681 taaatcttcg taaacgctgc cctgaatgat accaaacagc gcatttttgt ttccgagact
    19741 gtcaaaacgc tcacggctac gcttcgccca acgcagagac atctccatgg agcgttttgc
    19801 gtaatcccag tcagcaggat acggcgtaca ctcatcaaag atcatgacga tatccgaacc
    19861 aagatcgtac tgaatctcca ttgatttttc aggatcgagg aaaatcggat cgccgttgat
    19921 cgggttacgg aagtgcacgc cctgttcggt gattttacga atatcgccaa ggctgaagac
    19981 ctggaagccg ccggagtcgg tgaggatcgg ccccttccac tgcataaaat cgtgcagatc
    20041 gccgtgcagt ttcatgattt cctggcccgg gcgcagccac aggtggaagg tgttgccgag
    20101 gataatttgc gcgccagtgg cttcaacttc ttccggcgtc atccctttta cggtgccgta
    20161 ggtgccaaca ggcataaaac aaggcgtttc cactacgcca cgatcaaaga ccaggcggcc
    20221 acggcgtgcg cgaccgtcgg tggtgtccag ttcaaatttc attttttctc cactacgtca
    20281 gaaaaacagt ccaacgtttt aaaccagcgc cacggaatta ctccccgacg cgctcattaa
    20341 ttgcctgcgg attgtacgtg ataaacatcg catcaccgta actaaaaaag cgatatttct
    20401 cttctaccgc tgctttatag gcgttcatgg tgtgttgata accggcaaag gccgaaacca
    20461 gcataatcag cgtcgattcc ggcaggtgga agtttgtcac cagcgcatcg accactttgt
    20521 actggaagcc cggatagata aagatttggg tatcgtcaaa gaacggttcg ataagatcgt
    20581 ttttcgccgc ctgagccgcg ctttccagcg aacgtactga ggtggtgcca accgcaatca
    20641 cccggttacc gcgcgctttc gccgccagta ccgcgtctac cacatcctgc ggcacttcag
    20701 cgtattccga gtgcatgatg tgatcttcaa tggtgtcgac gcgcaccggc tggaaggtgc
    20761 ccgcaccaac gtgcaacgtc acaaacgcca tctccacgcc tttggcgcgt aatttttcca
    20821 gcaaaggttc gtcaaaatgc agacctgcgg tcggggctgc aaccgcgccc ggtttttcgc
    20881 tataaacggt ttgataaagt tcgcggtcag cgtcttcgtc cggacggtcg atatacggcg
    20941 gcagcggcat atggccgatg ctgttgagaa tatccagcac cgagcgttca tcattaaatt
    21001 cgacttcaaa cagtgcgccg tggcgcgcgg tcattgttgc gttaatactt tcgtcatcgc
    21061 ccagcagcag ttctgcgcca ggttttggcg ctttcgaggc gcgaatatgc gcaagaatgc
    21121 gtttgtcgtc gagcatccgt tcaaccagca cttcaatctt gccgccgctg gctttacgcc
    21181 caaacaggcg cgccgggatc acgcgggtat tattaaaaac cagaagatcg ccggggttga
    21241 gcttatcaag taaatcggtg aaagtaccgt gcgtcagcgc gcccgtcggc ccgtccagcg
    21301 acagtaaacg acagctactg cgttcaggca tgggatagtg ggcaatcagg gattcgggca
    21361 attcaaagga gaaatcggta acgcgcatga cactgactca gactaaaata agaggcgggt
    21421 agtctagtgc cggggcgctc tccctgcaac ctttaccccc ttttggatag aatataatcg
    21481 atgaattttt tagctcacct gcatttagcc catctcgcgg aaagctcgct ttccggcaat
    21541 ttactggctg atttcgtacg cggaaatcct gaagaaagtt ttccgcccga cgtcgtggct
    21601 ggcattcata tgcatcgacg tatcgacgta ttaaccgaca atctgccgga agtccgcgaa
    21661 gcacgggaat ggtttcgtag tgaaactcgc cgcgttgcgc ctattacgct ggatgtcatg
    21721 tgggatcact ttctttcccg ccactggtcg cagctgtcgc cggactttcc gctacaggaa
    21781 tttgtctgtt atgcccgcga gcaagtgatg acgattttgc cggactcacc gccacgtttt
    21841 atcaatctga acaattactt gtggtcagag cagtggctgg tgcgctatcg cgatatggat
    21901 ttcatccaga acgtgttaaa cggcatggca agccgccgcc cacgtctgga tgccctgcgt
    21961 gactcctggt acgatttaga cgctcattat gacgccctcg aaacccgctt ctggcagttt
    22021 tatccgcgga tgatggcgca ggcgtcacac aaggcgttat aaaacgttag ttcatccata
    22081 ccgtagccga aatggcaggc aaagccagaa tcccgtcagt cagttgccca tgcctttctt
    22141 tgcattgcca ttgcacggca ttgagcaacg gtgacgcggg tagcacgact tcacaagctt
    22201 cgccacggtt gattgccacc agtacacgct gctgattcag cacgcggaca aataccacca
    22261 cgttatcttc cgcatacagc acctgacagc cgccatgacg tagcgcctga cttttcttac
    22321 gcagcgcaat cattcgctgg tacagcgcga ataacgccgt atcctgcttt tccacctgcc
    22381 aggggaacgg tttacggcaa aacggatcgt ttttgccatc cagtcctact tcatcaccgt
    22441 aataaatgca cggtacacca ggccaggtga acagccagac caccgccagc ggcaggcgcg
    22501 caatatcccg accgagcagc gttttaaatc gcgcagtatc gtggctgtcg agctgattaa
    22561 acatacgtaa ttgttgttga tgagaaagcc ctgcgcggta attatccatc caggccatac
    22621 aggtttgggc atcaatttgc tgcggatcgt aagagatatc ggtattggca agaaatcccc
    22681 acaacgggaa tgtgaagcca cgatagttca tggcggcatc ttccacatcg gcctgtaacc
    22741 attgccgtgc atcgccaaaa tgttcgccga caatatacgc ttccggctgg gtttctttcg
    22801 ccgcttcggt gctcccagca acgtgctgca tattattgcg cgccccaccc gcctccccta
    22861 gcatatgcac cacatccagc cgccagccgt ccatactcca cggcgctttc agccaatggc
    22921 ggacaatact gtcttccccg cgataaattt cattcaccag actttccgac tgataatcca
    22981 gcttcggcaa gctggcatag ccaagccagt cgagcgccgt gccatcatca ctaaacgagt
    23041 accagtcgcg ccagggcgat tcagggttgt gacaagctcc ccccgtgcca cgattgtgcc
    23101 tatcaaacca ggcatgggaa tcgccactgt ggttaaacac gccatccagc cccagccgca
    23161 ttcccagctg ctgcgtattg tggcgtaagc gcagcaacgc cccatcaccg ccaaactgcg
    23221 gatcgacatg gcgataatcc tcggtatcgt atttatgtac gctgggagct ttaaacaccg
    23281 gattgagata cagcgccgtc acgccaagct ttttcagata cggcagtttt tcgcttatcc
    23341 cgtccagatc gccgccatag aacgttgatc cgcccgcctg cgccgtgacc ggttcatccc
    23401 agtcacgcaa gatgatctct tgtccggctg catgatggta atagacatga tcctgttcag
    23461 cttcacgagg aagactacgc gcaaaacgat cagggaagat ctgataaaaa atctgatccg
    23521 cagcccattg tgggccgata tccggtacat cgacggcaaa ctgctccagt cgtgccggcg
    23581 gcattcggct gaagccctgc ggtgtaaacc aacgctggcg atcgtgccac agcagtttga
    23641 aactgtaacg ccgccggggt tgtccgctgg agagatcaat cgccgcacgc catgcggtga
    23701 cgccaggctg ctgctgactg cgatgcttat gcatcgatac tgacatttct tcgttatcgt
    23761 gttctgtacg cagcataatg cgctgcggtg ggtcttcgcc cgtcagccac agtgtaatga
    23821 gcagttgatc tttgctttgt ttaacaaatg ggggcaccgg caggtgccat gcatttaaca
    23881 tcataattcc cctgtgatga aaatctgatc accttgccat agcctaatgc aggatcactc
    23941 atcatctgaa ggatatttgg gggaggagga cgggggcgga taactttcgg aatagccgga
    24001 tcaggatttc ctcctgaacc ggcttacaga acgcaaggat tactggtttt cagccagctg
    24061 acgatcgtgg cgacgcttaa acatccagcc aatcaacagc acaacaatcc acgcgaaacc
    24121 gacatacagc gagatacgcg tatccgggtg ataaccaatc aacccgataa taaagagcag
    24181 gaaaatcagc ccgccgatgg tcgttgctac cccacccggc actttaaatt tcagcgcctt
    24241 aacttcttct ggcggcaaac agcgacggaa ggcaatttgc gacagcagga tcataatcca
    24301 cacccacacc gtggcgaagg ttgccagcga agcgatcacc aggaagacgt tttccggcat
    24361 gatgtagttc agatacaccg caaacagcag cgcggtagtc atcaccagca ccgtaaccca
    24421 cggaataccg cgacgcgacg ttttgctgaa aattttcggc gcgctgccct gctctgccat
    24481 accgtggagc atacggccta cgccaaagac gtcactgtta attgccgata gcgaagcggt
    24541 cagcacaaca aagttaagaa tgctagcggc aaaggtaatg cccatatgct ggaacgtcag
    24601 cacgaacggg ctaccggcag tgccaacctg attccacggg tagatagaca taatgacgaa
    24661 cagcgtaccg acgtagaaca ccagaatacg catcggcacg gagttaatcg cacgcggtat
    24721 cgatttctca ggatctttcg cttcaccggc ggtaatcccg ataatttcga tcccaccgta
    24781 agcaaacatc accatttgca acgacatcac catcccaagc cagccgttac tgaagaagcc
    24841 gccgttgctc cacagattat gaataccggt cggttgcccg ccgttgccaa tcccccagat
    24901 gatgatgccg aaaccagcga caatcatgat gatgatggtg gcgactttaa agaacgagaa
    24961 ccagaattcc agctccccga agaccttcac gctcatcagg tttacggcgc aaatgatcag
    25021 caccacgctc agtacccaaa tccagtgcgg caccgtcggg aaccagacac ccatatagat
    25081 accaaaagcg gtcacatcgg cgatggcgac aataaggatt tcaaagcagt aggtccagcc
    25141 ggtaatgtaa cctgccagcg ggccgaggtt ttcctgcgca taacgcgaga aagagctggc
    25201 ggccgggtta tgtaccgaca tttcccccag cgcacgcata atgatatacg ccgcgatacc
    25261 accgataata taggccaaca acacgctcgg accggccatt ttgatggcgt ctgccgaacc
    25321 gtaaaacagc ccggtaccaa ttgctgaacc cagtgccata aagcgtatgt ggcgggtact
    25381 tagcccacgc tttagcttgt tcttactttc catcaattcc taacccatca acacaataaa
    25441 aaaccacggg gcatcgcagc cccgtggtta aaacaaatgt tcagtgattt agtgagcgct
    25501 ggaggtcacc tgacgacctg ccgcgcgatc ccagataatg gccagaacca ccatcaccac
    25561 tgttggcatt aaccacgcca gaccttgttc ggccagcggt aaacgctgcg cccaggacgg
    25621 taagatatcg ctgaatgcag atgctttgat cccgtcgaga ataccaaaaa gcaggctgat
    25681 aaacatcggc ggagcaatca cgcgggacga attatgccac catgagcgtg taaaacttaa
    25741 tacaaccagt gcgatacacg gcggataaat agcggtcagc accggtacgg agatctggat
    25801 cagctggctt aagccgaggt tagaaaccac catcgagaag ccgccgagga taaacaccag
    25861 cgtacgataa gagagcggta cgtactgggc aaagaattct gcacaagcac aggtcaggcc
    25921 aactgccgtt accaagcagg cgatgaagat taacgccgcc agcaggaagc taccgccgcc
    25981 gccaaaggtg tgctgaacgt aagcatgcag aatagcagcg ccgtttgcag actgatcgac
    26041 cagcgacgcg ctgtctgaac ccagacggaa cagcgccagg tacagcagag tcagaccaac
    26101 acccgccatc aggccagccc agacggtata acgggtcagc agacgcgctt cggtaacgcc
    26161 acgagaacgc gccgcgttaa caataacgat accaaacacc attgcgccca gcgtatccat
    26221 ggtcagatag ccgttaacga agccgttaga aaacgcagcg ttttgataag cctcagtcgc
    26281 cgtgctgata gaacccgccg gccagacaat cgcggcaaca gacaggatga ccagcgcgat
    26341 aattttcagc ggcgcaagga agttgcccac ggtatccagc agcttgcccg gatagagcga
    26401 aaccagaata acgatagcga aatagaccag gctgtagata aacagcggca gcgcggaatc
    26461 accagtcagc ggcgcaatcc ccacttcaaa ggaaacggta gctgtacgcg gcgtagcgaa
    26521 aagcggcccc accgccaggt aacaaaccgt tgccagcagc acgccagcga ctttaccgat
    26581 tggcgtgctg aggctgtcaa caccgccgcc aacttttgcc agcgccacta ccgttaatac
    26641 cggcaggcca acggcagtaa tgaggaagcc gaatgccgca gtccagacgt gttcgccagc
    26701 ctgcaagccg accattggag ggaaaataat gttacctgcg ccgacgaaca acgcaaatgt
    26761 cataaagccc agagcgatga tatcgcgcga tcttaattga tgggtcataa atcatactgc
    26821 ctgtggatgt ggtgttgtaa cgttaaattt ttgtgccaat ccctggggat gatacagcgg
    26881 tatatttaaa caattttagc gggaaatact cccaaccagg cgcggcgaag aatagttttt
    26941 cgatttaccg cgttaatgca gtcagtatga cagcacctga agcgcaattt aaacgcttat
    27001 aacgttttaa agcaaggagg gatctaaaaa ccagaggtat atagcgataa gaaatcacca
    27061 accagtataa aacactattt ccgtgaaaaa gacatggcta atcatgcgaa caaaaaatca
    27121 ccaaccgttg taaaatctaa cgcccgtatt ttgactgcaa agaaaataag ccagcatcga
    27181 ctggcttatg gcaataaaag atgacaaagg cggattaatc gctgtttttg gcaattaaac
    27241 gttccgggat aacaaaactg aaacgtgttc cttttcctac tgtactctca atattcaggc
    27301 gactttcgtg atgattcaca gcatgtttca cgatcgctaa ccctaatccg ctaccgccgg
    27361 tttgccggga acgcgcttta tcaacgcgat aaaaacgctc ggtcaggcgc ggaatatgct
    27421 ccggtgcaat gcccggtccg ttatcttcaa cgctaaattc ggcaccgtgc ggcactcgct
    27481 gccagcgtac ggtgatatgc gtgccttctg gagtatgatt cacggcgtta tagaccaggt
    27541 tcgaaatcgc actgcgtagc tgatcttcgt tgccagacac tttgaggccg ttatctatct
    27601 caaaggtaaa tgtctgtttt ttctgactca gagtctgagc ctcgcgctca acaacgcgca
    27661 gcatcatcgg cacatcaacc ttttcattga gcaattgcgt cggcgcggct tcaattttcg
    27721 acagcgttag caactgcttc accagccctt ccatccgctg ggtctgctcg cgcatggtgt
    27781 gcaacgcttt ttcgcgtacc gcgccttcca gcggctgctc atccatcatc tccaggtaac
    27841 cctgtaacac ggtcaatggc gtacgtaact catggctcac gttggcaaaa aagttacgcc
    27901 gcgccccttc cagttgatgc atttgcgtga catcacgcgc caccatcagc aactgtttgt
    27961 gggtataagg catgacgcga atttccagat gccgcccggt gttgagcacc agattgagcg
    28021 ggcgagaaaa atcacgcgtt ttcagatatt gcgtaaactc cgggtaacgc agtaggttaa
    28081 ggatgttctg cccgttatct tccggccagc gcaaaccaag aatttgttgc gccagaccgt
    28141 tacaccagaa aataccgctc tcttccgtgg tcagcaccac cgcgtcgggc agcgactccg
    28201 cgccgctacg aaagcgttta atcagattgc ccagttcacg gcggcgtttt ttatttcgca
    28261 gctgcatctg gtgtaagccg tatagtagcg gttcccagct accacgcccc ggtggcgggg
    28321 tcatactgcg atccacccac agccaccatg aaaggcgcaa taaattccag aaatgccaga
    28381 taagcagtcc tgttaccgat gccagcaaaa accagggcag gtaaccaaaa aatgcaccca
    28441 ggatgaaagc cgggaggcag caaagtagca gctccagcac cagccttttc cacgacagcc
    28501 gttccagcac gtaagatact cccgttaaga aatcataagc cctgctctgc gtccgatgag
    28561 caaggcgtta aaagcgggtt gaaaaacgat atcctgtacc gcgcacggtc tgcaccatgc
    28621 ggtcatgccc gccgggctcc agtgctttac gcaggcgacg aatgtggaca tcgaccgtgc
    28681 ggtcttccac atacacgtta gttccccaga cgtggtttaa cagctgctcg cggctgtaca
    28741 cgcgctcagg atgcgtcata aaaaagtgca gcagtttaaa ttctgtcggc cccatctcca
    28801 gcggctcttc gcccgccatc actcggtgag atgtcgggtc gagacttaat ccctgcatct
    28861 caatcacctc ttccaccgcc attggcgaaa tacggcgcat taccgctttg attcgcgcca
    28921 ccagctcctt cggcgaaaac ggcttggtga tatagtcatc cgcgccggtt tcaaggccgc
    28981 gcacgcgatc ttcttcttcc cctctggcgg tcaacatcac cactggaata tcccgggtca
    29041 tcgactcgcg cttaaggtgt ttgatgaact ggataccgga gccgccaggt aacatccagt
    29101 caaggagaat taaatccggc cagggttcat tcagttgatt cacagcactg tcataatctt
    29161 ccgcttcgac cggctgaaag ccattttgtt cgagcacgaa gcagaccatt tcacgaattg
    29221 gagcttcatc ttctacgacc agaatacgtc tcgccatgat ttgccctgtt gtaataaata
    29281 ggttgcgatc attaatgcga cgtcattatg cgtcagattt atgacagatt tatgaaaagc
    29341 tcgtcgcaca tatcttcagg ttattgattt ccgtggcgca gaaaaaagca aatggcacat
    29401 ctgtttgggt ataatcgcgc ccatgctttt tcgcctggga accgttatgc gcatccttca
    29461 cacctcagac tggcatctcg gccagaactt ctacagtaaa agccgcgaag ctgaacatca
    29521 ggcttttctt gactggctgc tggagacaac acaaacccat caggtggatg cgattattgt
    29581 tgccggtgat gttttcgata ccggctcgcc gcccagttac gcccgcacgt tatacaaccg
    29641 ttttgttgtc aatttacagc aaactggctg tcatctggtg gtactggcag gaaaccatga
    29701 ctcggtcgcc acgctgaatg aatcgcgcga tatcatggcg ttcctcaata ctaccgtggt
    29761 cgccagcgcc ggacatgcgc cgcaaatctt gcctcgtcgc gacgggacgc caggcgcagt
    29821 gctgtgcccc attccgtttt tacgtccgcg tgacattatt accagccagg cggggcttaa
    29881 cggtattgaa aaacagcagc atttactggc agcgattacc gattattacc aacaacacta
    29941 tgccgatgcc tgcaaactgc gcggcgatca gcctctgccc atcatcgcca cgggacattt
    30001 aacgaccgtg ggggccagta aaagtgacgc tgtgcgtgac atttatattg gcacgctgga
    30061 cgcgtttccg gcacaaaact ttccaccagc cgactacatc gcgctcgggc atattcaccg
    30121 cgcacagatt attggcggca tggaacatgt tcgctattgc ggctccccca ttccactgag
    30181 ttttgatgaa tgcggtaaga gtaaatatgt ccatctggtg acattttcaa acggcaaatt
    30241 agagagcgtg gaaaacctga acgtaccggt aacgcaaccc atggcagtgc tgaaaggcga
    30301 tctggcgtcg attaccgcac agctggaaca gtggcgcgat gtatcgcagg agccaccagt
    30361 ctggctggat atcgaaatca ctactgatga gtatctgcat gatattcagc gcaaaatcca
    30421 ggcattaacc gaatcattgc ctgtcgaagt attgctggta cgtcggagtc gtgaacagcg
    30481 cgagcgtgtg ttagccagcc aacagcgtga aaccctcagc gaactcagcg tcgaagaggt
    30541 gttcaatcgc cgtctggcac tggaagaact ggatgaatcg cagcagcaac gtctgcagca
    30601 tcttttcacc acgacgttgc ataccctcgc cggagaacac gaagcatgaa aattctcagc
    30661 ctgcgcctga aaaacctgaa ctcattaaaa ggcgaatgga agattgattt cacccgcgag
    30721 ccgttcgcca gcaacgggct gtttgctatt accggcccaa caggtgcggg gaaaaccacc
    30781 ctgctggacg ccatttgtct ggcgctgtat cacgaaactc cgcgtctctc taacgtttca
    30841 caatcgcaaa atgatctcat gacccgcgat accgccgaat gtctggcgga ggtggagttt
    30901 gaagtgaaag gtgaagcgta ccgtgcattc tggagccaga atcgggcgcg taaccaaccc
    30961 gacggtaatt tgcaggtgcc acgcgtagag ctggcgcgct gcgccgacgg caaaattctc
    31021 gccgacaaag tgaaagataa gctggaactg acagcgacgt taaccgggct ggattacggg
    31081 cgcttcactc gttcgatgct gctttcgcag gggcaatttg ctgccttcct gaatgccaaa
    31141 cccaaagaac gcgcggaatt gctcgaggag ttaaccggca ctgaaatcta cgggcaaatc
    31201 tcggcgatgg tttttgagca gcacaaatcg gcccgcacag agctggagaa gctgcaagcg
    31261 caggccagcg gcgtcacgtt gctcacgccg gaacaagtgc aatcgctgac agcgagtttg
    31321 caggtactta ctgacgaaga aaaacagtta attaccgcgc agcagcaaga acaacaatcg
    31381 ctaaactggt taacgcgtca ggacgaattg cagcaagaag ccagccgccg tcagcaggcc
    31441 ttgcaacagg cgttagccga agaagaaaaa gcgcaacctc aactggcggc gcttagtctg
    31501 gcacaaccgg cacgaaatct tcgtccacac tgggaacgca tcgcagaaca cagcgcggcg
    31561 ctggcgcata ttcgccagca gattgaagaa gtaaatactc gcttacagag cacaatggcg
    31621 cttcgcgcga gcattcgcca ccacgcggcg aagcagtcag cagaattaca gcagcagcaa
    31681 caaagcctga atacctggtt acaggaacac gaccgcttcc gtcagtggaa caacgaactg
    31741 gcgggttggc gtgcgcagtt ctcccaacaa accagcgatc gcgagcatct gcggcaatgg
    31801 cagcaacagt taacccatgc tgagcaaaaa cttaatgcgc ttgcggcgat cacgttgacg
    31861 ttaaccgccg atgaagttgc tagcgccctg gcgcaacatt ccgagcaacg cccactgcgt
    31921 cagcgcctgg tcgcgctgca tgggcagatt gttccccaac aaaaacgtct ggcgcagtta
    31981 atggtcacta tccagaatgt cactctggaa cagacgcaac gtaatgtcgc acttaacgaa
    32041 atgcgccagc gttataaaga aaagacgcag caacttgccg atgtgaaaac catttgcgag
    32101 caggaagcgc gcatcaaaac gctggaagct caacgtgcac agttacaggc gggtcaacct
    32161 tgtccacttt gtggttccac cagccacccg gcggtcgagg cgtatcaggc gctggagcct
    32221 ggtgttaatc agtctcgatt actggcgctg gaaaacgaag ttaaaaagct cggcgaagaa
    32281 ggtgcggcgc tgcgcgggca actggatgca ttaacgaagc agcttcagcg cgatgaaaac
    32341 gaagcgcaaa gcctccgaca agatgagcaa gcacttactc aacaatggca agccgtcacg
    32401 accagcctca atatcacctt gcagccacag gacgatattc aaccgtggct ggatgcacaa
    32461 gatgagcacg aacgccagct gcggttactc agccaacgac atgaattaca agggcagatt
    32521 gccgcgcata atcagcaaat tatccagtat caacagcaaa ttgaacaacg ccagcagcag
    32581 ctcttaacgg cattgacggg ttatgcactg acattgccac aggaagatga agaagagagc
    32641 tggttggcga cacgtcagca agaagcgcag agctggcagc aacgccagaa cgaattaacc
    32701 gcgctgcaaa accgtattca gcagctgacg ccgattctgg aaacgttgcc gcaaagtgat
    32761 gatctcccgc acagcgaaga aactgtggcg ctggataact ggcggcaggt tcatgaacaa
    32821 tgtctcgcat tacacagcca gcagcagacg ttacagcaac aggatgttct ggcggcgcaa
    32881 agtctgcaaa aagcccaggc gcagtttgac accgcgctac aggccagcgt ctttgacgat
    32941 cagcaggcgt tccttgcggc gctaatggat gaacaaacac taacgcagct ggaacagctc
    33001 aagcagaatc tggaaaacca gcgccgtcag gcgcaaacgc tggtcacaca gacagcagaa
    33061 acgctggcac agcatcaaca acaccgacct gacgggttgg ctctcactgt gacggtggag
    33121 cagattcagc aagagttagc gcaaactcac caaaagttgc gtgaaaacac cacgagtcaa
    33181 ggcgagattc gccagcagct gaagcaggat gcagataacc gtcagcaaca acaaacctta
    33241 ctgcagcaaa ttgctcaaat gacgcagcag gttgaggact ggggatatct gaattcgcta
    33301 ataggttcca aagagggcga taaattccgc aagtttgccc aggggctgac gctggataat
    33361 ttagtccatc tcgctaatca gcaacttacc cggctgcacg ggcgctatct gttacagcgc
    33421 aaagccagcg aggcgctgga agtcgaggtt gttgatacct ggcaggcaga tgcagtacgc
    33481 gatacccgta ccctttccgg cggcgaaagt ttcctcgtta gtctggcgct ggcgctggcg
    33541 ctttcggatc tggtcagcca taaaacacgt attgactcgc tgttccttga tgaaggtttt
    33601 ggcacgctgg atagcgaaac gctggatacc gcccttgatg cgctggatgc cctgaacgcc
    33661 agtggcaaaa ccatcggtgt gattagccac gttgaagcga tgaaagagcg tattcctgtg
    33721 cagatcaaag tgaagaagat caacggcctg ggctacagca aactggaaag tacgtttgca
    33781 gtgaaataac tattcagcag gataatgaat acagaggggc gaattatctc ttggccttgc
    33841 tggtcgttat cctgcaagct atcactttat tggctacggt gattggtagc cgttctggtg
    33901 gttgtgatgg tggtatgaaa aaagtcattt tatctttggc tctgggcacg tttggtttgg
    33961 ggatggccga atttggcatt atgggcgtgc tcacggagct ggcgcataac gtaggaattt
    34021 cgattcctgc tgccgggcat atgatctcgt attatgcact gggggtggtg gtcggtgcgc
    34081 caatcatcgc actcttttcc agccgctact cactcaaaca tatcttgttg tttctggtgg
    34141 cgttgtgcgt cattggcaac gccatgttca cgctctcttc gtcttacctg atgcttgcca
    34201 ttggtcgcct ggtttccggc tttccgcatg gcgcattttt tggcgtcggc gcgatcgtgt
    34261 tatcaaaaat tatcaaaccc ggaaaagtca ccgccgccgt ggcggggatg gtttccggga
    34321 tgacagtcgc caatttgctg ggcattccgc tgggaacgta tttaagtcag gaatttagct
    34381 ggcgttacac ctttttattg atcgctgttt ttaatattgc ggtgatggca tcggtctatt
    34441 tttgggtgcc agatattcgc gacgaggcga aaggaaatct gcgcgaacaa tttcactttt
    34501 tgcgcagccc ggccccgtgg ttaattttcg ccgccacgat gtttggcaac gcaggtgtct
    34561 ttgcctggtt cagctacgta aagccataca tgatgtttat ttccgttttt tcggaaacgg
    34621 cgatgacctt tattatgatg ttagttgggc tagggatggt gctgggaaat atgctaagtg
    34681 gcaggatttc aggacgttat tcaccactgc gcattgcagc agtgactgac tttatcattg
    34741 tactggcact gctgatgctc tttttctgcg gcggcatgaa aacaacgtcg cttatttttg
    34801 cttttatttg ttgcgcggga ttatttgccc tttcagcacc tctgcaaata ttgttactgc
    34861 aaaacgccaa aggcggagag ttattaggtg ccgcaggtgg gcaaatagcg tttaacctcg
    34921 gtagcgccgt cggcgcatat tgcggaggta tgatgctgac gctggggctg gcatataatt
    34981 acgtggcgct gcctgccgcc ctgctttcgt ttgctgcgat gtcatcgttg ctgctgtatg
    35041 gtcgctataa gcgccagcaa gcggcggata gtccggtgct ggcgaaacca ctggggtaga
    35101 ttactgtcac ggtgcgcgtt gcttattgcc ggatgcggcg tgaacgcctt atccgcccta
    35161 cgcggttctg gcacattttg taggcctgat aagacgcggc aagcgtcgca tcaggcatga
    35221 tgcgccaatt gcctacgttt tttactcttg tggccataac cacgcagcgc cgcgtacgcc
    35281 gctggaatca ccgtgcttcg ccttacgcac cggcgtttca cattcgccgc cgaagacaaa
    35341 ttgtttaatc aactgcccaa ccgtttgata taaacggtct acattgctca tcccgccccc
    35401 caggacaatc acatccggat cgagaatatt cacgacatgt gccagcgatt ttgccagccg
    35461 cagctcgtag cgacgcaatg ccagttccgc taccggatcg ctttcttcaa ccaggcggat
    35521 aatttcactg cctttcagcg catgtccgct caaacgacga taatccgtcg cgaatcccgt
    35581 gcccgaaata aaggtttcaa tacaaccttg tttaccgcaa taacaaggga cttcctcgcg
    35641 ataacgcagc tcgtcttcat ccatccacgg tagcggattg tgtccccact cgcctgccgt
    35701 gccattgccg ccgatatgcg cccgcccatt taatgccacg cccgcgccgc atcccgtgcc
    35761 gataatcacg gcaaataccg tctgcgctcc cgctgccgcg ccatctactg cttctgaaac
    35821 cgccagacag ttggcatcat ttgccagccg cacttcccgc tgcaacctcg cgcttaagtc
    35881 tttatcgaat ggctgaccgt tgagccaggt tgaattggca ttcttcacca caccggtgta
    35941 gggcgaaatc gaacctggaa tgcccatacc taccgttccg cgctgccctg tcgcctgctc
    36001 cgccatatca accaacgtgg cgatcgtttc aatagtctgc cggtaatcat cacgcggcgt
    36061 gggcagacga tggcggtaca actgctcccc tgcatcgccc agtgcaatca cttcagtttt
    36121 ggtgccgcct aaatcgatac ctatacgcac tgtactctcc ttattttttt caatatcaat
    36181 agcgtagaga cggacaaccg gattggcaat gcaaggccgc cgacaattcg ttatcatgcc
    36241 cgctaaattt aacgacaagg ccgtggaaat tatcatgctg tggttcaaaa atttaatggt
    36301 ttaccgtctt agccgcgaga tttcgctgcg tgcagaagag atggaaaaac agctagcctc
    36361 gatggcattt accccatgcg gcagccagga catggcgaag atgggctggg ttcctccgat
    36421 gggatcgcac agcgatgcgt taacgcacgt tgccaatggt caaattgtta tctgcgcgcg
    36481 caaagaagaa aaaatcctcc cgtctccggt gattaaacag gcgctggaag cgaaaatcgc
    36541 caaactggaa gcggaacagg cgcgtaagct gaagaaaacc gaaaaagatt cgctgaaaga
    36601 cgaagtgctg cactctctgc tgccgcgtgc tttcagccgt tttagccaga caatgatgtg
    36661 gatcgacacg gttaacggtt tgattatggt ggactgcgcc agtgccaaaa aagcggaaga
    36721 tacgctggca ttactgcgta aaagcctggg gtcgttaccg gttgtaccat tgagcatgga
    36781 aaacccgatt gaactgacgc tgaccgaatg ggttcgctcc ggtagtgcgg cacagggctt
    36841 ccagctgctt gatgaggccg agctgaaatc gttgctggaa gatggcggcg tgatccgcgc
    36901 gaagaaacaa gatctgacca gcgaagagat caccaatcac attgaagccg gaaaagtggt
    36961 gactaaactg gcgctcgact ggcagcagcg cattcagttt gtgatgtgcg acgatggttc
    37021 gctcaagcgt cttaagttct gcgacgagct gcgcgatcaa aacgaagata tcgaccgtga
    37081 agatttcgcc ctgcgttttg atgccgattt catcctgatg actggtgaac tggcagcgtt
    37141 aattcaaaac ctgattgaag gattaggtgg cgaagcacaa cgttaattgc tgattttcct
    37201 ttaatgccgg atgcgacgcc tgccgcgtct tatccggcgt acgaagccac accaggcata
    37261 taattattcg ctacggcgag caataatttt tagcgcagca atattatgcg ttttacgccg
    37321 taactgatct aatcgcctca tccctattcg cctgtcagca gcacgcagaa tattcactaa
    37381 cccccattta tcgctggtaa attgttttgt taacagctcc gcttttgggg tatcaatata
    37441 ttcacgcaat aaatcagaaa ttgaacaaat atccgtcagg taaggatacc agcaagtttc
    37501 accgtgaaaa ttacgcactc cagcgttacc tgcaataaag tctttcgggt aatcccagat
    37561 cacgttttta tccaacgtga tccaataacg cggtaaatcg gtacttccac cattttggct
    37621 gcgcatgaga taacgggtac agtgaatttg caggttgatc gttggcgtca gtaagtcata
    37681 gatctcccgc tgtaacttgc tccatggatg ttgtgtcatt gtttttcctc aagccggtga
    37741 cgcgcttcac tgcgaatttt ccgcgttgcc ccggttttca ctaaatcacg taactgcgaa
    37801 agatcgggtt gttcgacaaa taagagtaat gatagatcct cttttaacca cgccgttttc
    37861 acatcggcag ccagctgtgg attattaata gccagcgacc gatcctgaga ctcagtcaat
    37921 gtcgttaaca acgacgccgg ggtatgtgcg ttgcgtaatg ccgtttggcg caaatgatca
    37981 ttg
//
LOCUS       DAJVQG010000021        37440 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJVQG010000021 DAJVQG010000000
VERSION     DAJVQG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37440
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.021"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..496
                     /gene="gstA"
                     /locus_tag="OQM83_000985"
     CDS             <1..496
                     /gene="gstA"
                     /locus_tag="OQM83_000985"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCS1519305.1"
                     /translation="ENGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQ
                     LLAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEA
                     LKDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSA
                     EGLK"
     gene            complement(540..1403)
                     /gene="pdxY"
                     /locus_tag="OQM83_000986"
     CDS             complement(540..1403)
                     /gene="pdxY"
                     /locus_tag="OQM83_000986"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCS1519306.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1462..2736)
                     /gene="tyrS"
                     /locus_tag="OQM83_000987"
     CDS             complement(1462..2736)
                     /gene="tyrS"
                     /locus_tag="OQM83_000987"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCS1519307.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2865..3521)
                     /gene="pdxH"
                     /locus_tag="OQM83_000988"
     CDS             complement(2865..3521)
                     /gene="pdxH"
                     /locus_tag="OQM83_000988"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCS1519308.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3580..3909)
                     /gene="mliC"
                     /locus_tag="OQM83_000989"
     CDS             complement(3580..3909)
                     /gene="mliC"
                     /locus_tag="OQM83_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCS1519309.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGVRYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4007..5116)
                     /gene="anmK"
                     /locus_tag="OQM83_000990"
     CDS             complement(4007..5116)
                     /gene="anmK"
                     /locus_tag="OQM83_000990"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCS1519310.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5390..5857
                     /gene="slyB"
                     /locus_tag="OQM83_000991"
     CDS             5390..5857
                     /gene="slyB"
                     /locus_tag="OQM83_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCS1519311.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5904..6338)
                     /gene="slyA"
                     /locus_tag="OQM83_000992"
     CDS             complement(5904..6338)
                     /gene="slyA"
                     /locus_tag="OQM83_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCS1519312.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6539..6775
                     /locus_tag="OQM83_000993"
     CDS             6539..6775
                     /locus_tag="OQM83_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCS1519313.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6778..7635
                     /locus_tag="OQM83_000994"
     CDS             6778..7635
                     /locus_tag="OQM83_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1519314.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7656..9647
                     /locus_tag="OQM83_000995"
     CDS             7656..9647
                     /locus_tag="OQM83_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCS1519315.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9648..10169)
                     /gene="sodC"
                     /locus_tag="OQM83_000996"
     CDS             complement(9648..10169)
                     /gene="sodC"
                     /locus_tag="OQM83_000996"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCS1519316.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10250..11146)
                     /locus_tag="OQM83_000997"
     CDS             complement(10250..11146)
                     /locus_tag="OQM83_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCS1519317.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11195..11434)
                     /locus_tag="OQM83_000998"
     CDS             complement(11195..11434)
                     /locus_tag="OQM83_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCS1519318.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11537..12136
                     /gene="nemR"
                     /locus_tag="OQM83_000999"
     CDS             11537..12136
                     /gene="nemR"
                     /locus_tag="OQM83_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCS1519319.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12173..13270
                     /gene="nemA"
                     /locus_tag="OQM83_001000"
     CDS             12173..13270
                     /gene="nemA"
                     /locus_tag="OQM83_001000"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCS1519320.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13351..13758
                     /gene="gloA"
                     /locus_tag="OQM83_001001"
     CDS             13351..13758
                     /gene="gloA"
                     /locus_tag="OQM83_001001"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCS1519321.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13861..14508
                     /gene="rnt"
                     /locus_tag="OQM83_001002"
     CDS             13861..14508
                     /gene="rnt"
                     /locus_tag="OQM83_001002"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCS1519322.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14601..19170
                     /locus_tag="OQM83_001003"
                     /pseudo
     CDS             14601..19170
                     /locus_tag="OQM83_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19221..19568)
                     /gene="grxD"
                     /locus_tag="OQM83_001004"
     CDS             complement(19221..19568)
                     /gene="grxD"
                     /locus_tag="OQM83_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCS1519323.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            19902..20717
                     /gene="mepH"
                     /locus_tag="OQM83_001005"
     CDS             19902..20717
                     /gene="mepH"
                     /locus_tag="OQM83_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCS1519324.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20845..21426
                     /gene="sodB"
                     /locus_tag="OQM83_001006"
     CDS             20845..21426
                     /gene="sodB"
                     /locus_tag="OQM83_001006"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCS1519325.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21572..22741)
                     /locus_tag="OQM83_001007"
     CDS             complement(21572..22741)
                     /locus_tag="OQM83_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1519326.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(22907..22996)
                     /gene="ynhF"
                     /locus_tag="OQM83_001008"
     CDS             complement(22907..22996)
                     /gene="ynhF"
                     /locus_tag="OQM83_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCS1519327.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23295..24320
                     /gene="purR"
                     /locus_tag="OQM83_001009"
     CDS             23295..24320
                     /gene="purR"
                     /locus_tag="OQM83_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCS1519328.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24317..25249)
                     /locus_tag="OQM83_001010"
     CDS             complement(24317..25249)
                     /locus_tag="OQM83_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1519329.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25362..26573
                     /locus_tag="OQM83_001011"
     CDS             25362..26573
                     /locus_tag="OQM83_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCS1519330.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            26864..28012
                     /gene="cfa"
                     /locus_tag="OQM83_001012"
     CDS             26864..28012
                     /gene="cfa"
                     /locus_tag="OQM83_001012"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCS1519331.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28052..28693)
                     /gene="ribE"
                     /locus_tag="OQM83_001013"
     CDS             complement(28052..28693)
                     /gene="ribE"
                     /locus_tag="OQM83_001013"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCS1519332.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            28908..30281
                     /gene="mdtK"
                     /locus_tag="OQM83_001014"
     CDS             28908..30281
                     /gene="mdtK"
                     /locus_tag="OQM83_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCS1519333.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30322..31578)
                     /locus_tag="OQM83_001015"
     CDS             complement(30322..31578)
                     /locus_tag="OQM83_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCS1519334.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            31886..31962
                     /locus_tag="OQM83_001016"
     tRNA            31886..31962
                     /locus_tag="OQM83_001016"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31920..31922,aa:Val,seq:gac)
     gene            31967..32043
                     /locus_tag="OQM83_001017"
     tRNA            31967..32043
                     /locus_tag="OQM83_001017"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32001..32003,aa:Val,seq:gac)
     gene            32151..32456
                     /locus_tag="OQM83_001018"
     CDS             32151..32456
                     /locus_tag="OQM83_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCS1519335.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32582..34186
                     /locus_tag="OQM83_001019"
     CDS             32582..34186
                     /locus_tag="OQM83_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCS1519336.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(34198..35010)
                     /locus_tag="OQM83_001020"
     CDS             complement(34198..35010)
                     /locus_tag="OQM83_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1519337.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35014..35799)
                     /locus_tag="OQM83_001021"
     CDS             complement(35014..35799)
                     /locus_tag="OQM83_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCS1519338.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35796..36464)
                     /locus_tag="OQM83_001022"
     CDS             complement(35796..36464)
                     /locus_tag="OQM83_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCS1519339.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36528..37166)
                     /locus_tag="OQM83_001023"
     CDS             complement(36528..37166)
                     /locus_tag="OQM83_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCS1519340.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37179..>37440)
                     /locus_tag="OQM83_001024"
     CDS             complement(37179..>37440)
                     /locus_tag="OQM83_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCS1519341.1"
                     /translation="KLMQTKDMRNEHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHAS
                     LTMFYKEMGWDPQLGCPTRETLQRLGLEDIAADLAAHNLLPA"
BASE COUNT         8943 a         9849 c         9306 g         9342 t
ORIGIN      
        1 cgaaaacggt gacgattact ttgccgttaa ccctaagggg caggtgccag cattgctgct
       61 ggatgacggt actttgctga cggaaggcgt agcgattatg cagtatcttg ccgacagcgt
      121 ccccgaccgc cagttgctgg caccggtaaa cagtatttcc cgctataaaa ccatcgaatg
      181 gctgaattac atcgccaccg agctgcataa aggtttcaca cctctgtttc gcccagatac
      241 accggaagag tacaaaccga cagttcgcgc gcagctggat aagaagctgc aatatgtgaa
      301 cgaggcactg aaggatgagc actggatctg cgggcaaaga tttacaattg ctgatgccta
      361 tctgtttacg gttctgcgct gggcatacgc ggtgaaactg aatctggaag ggttagagca
      421 cattgcggca tttatgcaac gtatggctga acgtccggaa gtacaagacg cgctgtcagc
      481 ggaaggctta aagtaatagc catcggccca caggtcgttg tgggccgaaa tgtgatattt
      541 cagagctttg ttgcgctgaa gtaatgttct ggtttggcaa tacgatcctg agcagccacc
      601 acttgcagct catattcctg cattgctttg gtggtcacca tgatttcgta gactgcagcg
      661 gtcacatgtt ccaggacctc ctgcagcgtt gccccctgaa gcagtttcac cagcagtaaa
      721 ccgctcgtca catcaccaac acctaccggc tggcgcatac caaaatccac cagtggacgg
      781 ctgatatgcc aggcttcatc ggcggtgacc agcagcattt caaaacggtc acggctgtag
      841 ccagctcgcg ccaggtgttt aaccaacaca atttgtggcc cttgcgcaat gagttcgcgc
      901 gctgccagaa ccgcttcttc gacgttattt accgcatgct cacagagtat ttccagctca
      961 accagattcg gcgcaatgat atcgctggca ggcaaaccgt gccgcacatg aaactctgcg
     1021 acacccggtg caacgataca gcctttttcc ggatgcccca ttaccggatc gcaaaaatat
     1081 ttcgcctgcg gattcgctgc tttcacctga cggacgatac cgaggatatg ttcaccctgc
     1141 tccgccgatc ccagatagcc acttaacacg gcatcacagg tgtgtaattt atcaatagcg
     1201 gcaatgcctt gcacaatttc ggttaaatgg ctgggcggca tcacgcagcc agtccatttg
     1261 ccgtattggg tgtgattaga aaattgaacg gtgttcagcg gccagacgtt cgcgcccagg
     1321 cggcgcatcg gaaactctgc cgcactgtta cccgcatgac cataaacaac gtgagactgg
     1381 atagcgagaa tatttttcat catgttccct gtataaaaac cagggggagt gatttctcac
     1441 tccccctttc cacttaatgc attatttcca gcaaatcaga cagtaattct ttttgccgcg
     1501 acgcagtaag gtaaaacgac caaacagacg atcttcttct ttaaagaagt attcaggatc
     1561 ggactgtttt tcaccgttaa tggtgatggc attggaggcg atagttttac gcgcctgacc
     1621 acgggaaggt tgcagttcag aatcgaccag cgcctgcatc aggtctgcgc ccttttccat
     1681 ctcaaccatc ggtacgccgt cctgcgccag ctgttcgaag tccgcttcac tcagcgcact
     1741 caaggaaccg ctgaacaggc attcggtaat acgttttgcc gcctgcaaac cttcttcacc
     1801 gtgaaccaga cgagtcacct gctccgccag tacatactgg gcgcgcggtg ctttaccgct
     1861 gtttttatct tcttcttcca gggcgttgat ctcttcaatg ctcataaagg tgaagaactt
     1921 caggaagcgg taaacgtcgg catccgcagt gttgatccag aactggtaga atttgtacgg
     1981 gctggttttc ttcggatcca accagactgc gccgccttca gttttaccaa atttggtgcc
     2041 gtctgctttg gtgatcagcg gaacggtcag gccaaacacc tgattctgat gcagacgacg
     2101 ggtcaggtcg ataccagaag tgatgttacc ccactggtca gaaccaccaa tttgcagcac
     2161 cacaccgtac tgtttgttca gacaggcgaa gtcataaccc tgcaacaggt tgtaggaaaa
     2221 ctcagtgaac gaaatcccct gatcttcacg gttgagacgc tgcttaaccg cttctttgtt
     2281 gatcatctgg ttaacggaga agtgtttgcc aatatcgcgc aggaaggtca gcacattcat
     2341 attgccgaac cagtcatagt tgttcgccgc gatagcagag ttttctccac agtcgaaatc
     2401 aaggaacggg gcaacctgct tgcggatttt gtccacccac tcctgaacag tttcttcggt
     2461 gttcagctta cgctcggcag ctttgaagct cgggtcgcca atcagacccg tcgcgccgcc
     2521 taccagcgca accggcttgt ggcccgcctg ctggaagcgt ttcaggcata acaatggaac
     2581 aagatgcccc aaatgcaagc tgtcagcggt aggatcgaag ccgcaataga gcgcgatcgg
     2641 gccttgcgcc agtcgctctg ctaacgcttc ctcgtccgtc acctgggcta ccagcccccg
     2701 ctcttgcaat tgtttaatca agttactgct tgccatcaaa atctccatgt atataacgac
     2761 tgcacctttg ccggtacacg acttttcgcc agatgcgaaa gagacataga ataaagtgcc
     2821 agaatcagga gtaccagcga ttaaagcaag atttttgcat cttttcaggg tgcaagacga
     2881 tcaatcttcc acgcatcatt ttcacgctgg tacaaaaagc ggtcatgcag gcgatgctca
     2941 ccgccctgcc agaactcaat ctgttcaagg ctgacgcgaa aaccgcccca aaagctcggc
     3001 aatggcactt cgccctgttg aaacttctgc ttcagctcca ggaatttact ttcaaggata
     3061 ccgcgggcag aaatgcgact ggactgcttc gaaacccatg caccaatctg gctatcacgc
     3121 gggcggctat gaaaatattt catcacttcg agagtcgaaa gtcgttccgc tttaccgatc
     3181 accatcacct ggcgctcaag ggtatgccac gggaacagca ggctaacgcg cggattattt
     3241 tcaatttgat gtgctttacg gctgccgagg ttggtgtaaa acaccatgcc tttttcgtcg
     3301 taatgtttga gtaaaacgat gcgttgataa ggctgagcat gttcatccac ggtagcgacc
     3361 accatcgcgg tagggtctgc cagtttggct tcacacgcct gagagagcca acgttcaaaa
     3421 agggttaatg gatcggcggg aagatcgcga cggcgtaacc cgcctttggt gtattcacgg
     3481 cgcagatgcg cgatttgctg caattcgtcg ttatcagaca tggttttctt tacggattgt
     3541 cagtgggtga cgctattgtg cgccgcccct ggaaaaatct caacgctgtg gattttgtaa
     3601 ctgacagtta ttcaatacga tgcggtcgcg tttatagaca gtcgcttcat cgcctttcga
     3661 ccagaaaaca tagattccgt cagtgtaacg tacaccagac gctgaaatgc cctgtttgag
     3721 atgcagtagt tgattatcgt aaacaaaact gacttcctgg cgcggattat tcagtttgac
     3781 cgtcaacggt ttttcatcac actggtattc cagcgtagcg gtttgcatgt gctcaaccag
     3841 ttgattgaac gcgctacagc cggtgagaag caccggcaaa cagatcagta acagtttttt
     3901 catagacatt tcccgaagac tttcctggtc tggagggcaa tacgccctcc ctaacgtttc
     3961 aagtgtaacg gcagacgcgg taagaaaaat tcagttaact ctgatatcac gggttagcag
     4021 ggaaaatagc ccccagtacc gtctcctggc ttgcgccagt gacggaaggc agatttcctg
     4081 gtaatcccgc cagcgtccgc caggcaagcc aggcgaatgc caatgcttcc atgtcatcgc
     4141 cactaatgcc aacggcatcg gtggtggtga cttctgtgcc tggcagtaat gccgccagac
     4201 gcgccatgag tagcgggtta cgactacctc caccacatac catcaatcgt tcgcagccac
     4261 cgctcaacaa aacttgttca gaaatggtca cggcggtgag ttctgccagt gtggcctgaa
     4321 catcacgggg atcaacaccc ggaaaatggc gtaaatggcg ctccagccag ccgtagttaa
     4381 agtattcgcg tccggtgctt ttcggtgcag gttgcgagaa atatgggtcg ctgagcatat
     4441 tttgcagcag tgggagaata actttacccg cccgtgccca ctcggcatct ttatcgtaag
     4501 gtttaccggc ctgacgccag atccaggcat ccatcagcat gttaccagga ccggtatcgt
     4561 agccgccaac cggctgccca ggaatgagca gtgacagatt ggcgatgccg ccaatattga
     4621 gcaccattcg tcgctcggtt gggtgagcca gcaaggcatg atggaacgca ggtaccagcg
     4681 gtgcgccttg tcctcccaag gcaatatcgc gacggcgaaa atcaccgaca accgtaattc
     4741 cggtgcgtgc cacaatttga ttgttatcgc caatctgtaa agtgtgtggt gccacgcccg
     4801 tcggttcatg ccagacggtt tgaccgtgac agccgatcgc aactatatct ctcgcctgca
     4861 ggttttgttc cttaagcaag gcattgaccg catcagcaaa aagtcgcccg agttgagtat
     4921 caagctgtcc aaactgcgaa agtgtaagct gctggccctg gcaaatatcc agtacagcct
     4981 gtttcagaga caccgggatc ggccagctca agcttgccag ctgtgcgacc cggtgttcat
     5041 ctattgtcgc caacacaaca tcaacaccat caaggctggt gcctgacata acgccaataa
     5101 agcggcccga tttcatagtt catccttttt caatctgacg tttgcgcacc actcaaacat
     5161 aaacttttcg tggataccat gcggaatgac cgatttttac cgttggtagt aaaacattat
     5221 cttcaaatca ataatcatca tgaatgtttt gtttataatt ggttgatcct actttcatta
     5281 tgatttgctc atatttggta gaacatgtaa ccatggattc acatatgcca tatactttga
     5341 ccatgaggga tgcttgcgtg gcgtttcatg gtgaacagga gatttttcaa tgattaaacg
     5401 cgtattggtt gtttcaatgg taggtctgtc tcttgtcggt tgtgttaata acgacaccct
     5461 gtcaggggat gtttataccg cttctgaagc gaaacaagta cagaatgtca gctatggcac
     5521 catcgttaac gtacgtccgg tacagattca gggcggtgat gattccaacg ttatcggtgc
     5581 aattggcggt gctgttcttg gtggtttcct ggggaatact gttggtggcg gaaccgggcg
     5641 ttctctggct actgcagcag gcgctgttgc aggtggcgta gctggtcagg gcgtacagag
     5701 tgcaatgaac aaaacgcagg gtgtcgagct ggaaattcgt aaagacgatg gtaataccat
     5761 catggtggta cagaaacaag gcaacactcg tttctctccg ggccaacgtg tcgtactggc
     5821 cagcaatggc agtcaggtga ccgtttctcc gcgctaaata agtttgcgtg tggtcaggtt
     5881 actgaccaca cgcccccttc atttcaccct ttggcctgta actcaatgat attatgctca
     5941 agttttgcga tgagcgtaat cagttgctcc agttcctctg cggagatgcc atgtaatatt
     6001 tccgcgcggg ttttgttaat aacagcttcc atttcgctga tcagtggctc tgccttttcc
     6061 gtcagtttaa tacgtttagc ccgacgatcg ctggcacaag tttgacgcga aattaaccct
     6121 ttttcttcca gttggtccag agtacggacc agtgatggct gctcgatgcc aatcgctttt
     6181 gccagttgaa tttgcgactg gtctggaggt aactgatgga tattgtgtaa cgtaacccaa
     6241 tgggtttgtg ttaactccag cggtttcagg cgatggtcta tcagagcacg ccatatgcgc
     6301 accaaccgtg ccagatcaga acctagtggc gattccaatt tcatctcctt ataattagct
     6361 tgctaagata ttatgcggct tttagaatag tgtgcagcaa ttgtattgct aaaacaaatg
     6421 tattgctgca tttggttacc gtcagacata tttttcagaa attgcgcgta aatttttcgc
     6481 acttaaagaa tatttattaa tctaacgcaa tatattcggt cgtaaaggaa tctactttgt
     6541 gaagtttatg ctcaatgcaa caggattgcc cttacaagat ctggtgttcg gtgcgtccgt
     6601 ctattttcct ccgtttttca aagcattcgc gtttggattc gtcatctggc ttgtcgtaca
     6661 ccgcctgctt cgtggctgga tctacgccgg tgacatctgg catcccttgt taatggattt
     6721 atcgctgttt gcgatttgcg tttgccttgc tctggcaata ctgattgcgt ggtaactatg
     6781 tcaattaaaa caattaagta tttctcaaca atcattgtag cggtagttgc ggttcttgcc
     6841 ggatggtggc tgtggaatta ttacatgcaa tctccgtgga cacgcgacgg gaaaatacgc
     6901 gcagaacagg tttctatcac cccacaggtg tcgggacgta ttgttgagct gaatataaaa
     6961 gacaaccagc tggttaacgc aggggatctt ctgctcacga tcgataaaac gccctttcag
     7021 atcgccgagc tgaacgcaca ggctcagctg gcgaaagcgc aatctgacct cgcaaaagcc
     7081 aataacgaag cgaatcgtcg tcgtcatctc tcacaaaatt ttatttctgc cgaagaactc
     7141 gataccgcta acctcaatgt taaagcgatg caggccagtg ttaatgccgc acaggccacg
     7201 ctgaaacaga cacaatggca actggcgcaa acggaaattc gcgccccggt gagtggacgg
     7261 gtgactaacc tcactacccg catcggcgat tacgctgaca ccgggaaacc gctttttgcc
     7321 cttgtcgaca gccactcgtt ttacgtcatt ggttattttg aagaaaccaa gttgcgccat
     7381 atccgcgaag gtgcaccagc acaaattacc ctctatagtg ataacaaaac gttacagggt
     7441 cacgtttcca gtatcggtcg ggcgatttat gatcagagcg ttgaaagtga ctcaagcctt
     7501 attccggatg tgaaacctaa cgtcccctgg gttcgtctcg cccaacgcgt tcccgtccgc
     7561 tttgcgctgg ataaagttcc aggcgatgtc acgctggtgt cgggcaccac ctgtagcatt
     7621 gccgtaggtc aataatgaac gcatcgtaat ggtccttgcg caatttgccc tggttcaggg
     7681 ccacgctggc gcaatggcgt tatgcgttac gcaataccat tgccatgtgt ctggcgctga
     7741 cggttgccta ttatttgaat ctggatgaac cctattgggc gatgacctcg gctgcagtgg
     7801 ttagctttcc caccgttggc ggtgtcatca gcaaaagcct cggacgcatc gctggcagtt
     7861 tactgggagc cattgcggca ctgcttcttg ccggacatac gctcaatgag ccgtggtttt
     7921 ttctattgag catgtcggcg tggcttggct tttgtacctg ggcctgtgcg cacttcacga
     7981 ataacgtcgc gtatgcattt caactggcgg gctacacggc tgccatcatc gcctttccga
     8041 tggttaatat tactgaggcc agccagctgt gggatatcgc tcaggcgcgc gtttgcgagg
     8101 tgatagtcgg tattttgtgc ggcggcatga tgatgatgat cctgcctagc agttccgatg
     8161 ctactgccct tttaactgca ttgaaaaaca tgcacgcccg attactggaa catgccagtt
     8221 tactctggca gcctgaaaca aacgatgcca ttcgtgcagc acatgagggg gtgattgggc
     8281 agatactgac catgaatttg ctgcgtatcc aggctttctg gagccactat cgttttcgcc
     8341 agcaaaacgc gcgccttaat gcgctactcc accagcaatt acgtatgacc agtgtcatct
     8401 ccagcctgcg acgtatgttg ctcaactggc cctcaccgcc aggtgccacg cgagaaattc
     8461 tcgaacaatt gctgactgcg ctcgccagtt cgcaaacaga tgcttacacc gtcgcacgta
     8521 ttatcgcccc gctacgcccg accaacgtcg ccgactaccg gcacgtcgcc ttctggcagc
     8581 gactacgtta tttttgccgc ctttatctgc aaagtagtca ggaattacat cgtctgcaaa
     8641 gcggtgtaga tgatcatgcc agactcgcac ggacatccgc tctggctcgt cataccgaca
     8701 acgccgaagc tatgtggagc gggctgcgta cattttgtac gttgatgatg attggcgcat
     8761 ggagtattgc atcgcaatgg gatgccggtg ccaatgcatt aacgctggca gcaattagct
     8821 gcgtactcta ctccgccgtc gcagcaccgt ttaagtcgtt gtcactgctg atgcgcacgc
     8881 tggtgttact ttcgctattc agctttgtgg tcaaatttgg cctgatggtc cagattagcg
     8941 atctgtggca atttttactg tttctctttc cactgctggc gacaatgcag cttcttaaat
     9001 tgcagatgcc caaatttgcc gcactgtggg ggcaactgat tgtttttatg ggttcgttta
     9061 tcgctgtcac taatcccccg gtgtatgatt ttgctgattt tcttaacgag aatctggcaa
     9121 aaatcgttgg cgttgcgctg gcttggctag cgttcgccat tctgcgtcca ggatcggatg
     9181 ctcgtaaaag ccgccgccat attcgcgcgc tgcgccggga ttttgtcgat cagctaagcc
     9241 gccatccaac actgagtgga agcgaatttg aatcgctcac ttatcatcac gtcagtcagt
     9301 tgagtaacag ccaggatgcg ctggctcgcc gttggttatt acgctggggt gtagtgctgc
     9361 tgaactgttc tcatgttgtc tggcaattgc gcgactggga atcgcgttcc gatccgttat
     9421 cgcgagtacg ggataactgt atttcactgt tgcggggagt gatgagtgag cgtggcgttc
     9481 agcaaaaatc actggcggcc acacttgaag aattacagcg gatttgcgac agccttgccc
     9541 gtcatcatca acctgccgcc cgtgagctgg cggcaattgt ctggcggctg tactgctcgc
     9601 tttcgcaact tgagcaagca ccaccgcaag gtacgctggc ctcgtaatta cttaattaca
     9661 ccacaggcat agcgttcacc gccaccgccc agcggtttag gttgatcgga catattatcg
     9721 ccgccaacgt ggaccatcag cgccttgtct ttgatttcat ccagtgattt cagacgaggc
     9781 gcgatgacgg catcggtagc tttgccgtca ttattgacga ccagtgcagg cagatcgcct
     9841 aaatgcccgg taccttctgg cccttcatgt ttaccggtat tttgtggatc aagatgcccg
     9901 cctgcggatt ccgcggcgct ggctttgcca tctttggtgg ctggctggca gcttcctttg
     9961 gcatgaatat ggaagccatg ttcaccgggg ggtaatgctt tcagatcggg tgaaaactcc
    10021 agacctttat cggtttcagt aatggtgacg ctaccaattg actgcccaac cccttgcgac
    10081 gtgacgaggt tcatctcgac tttttcactg gcagcttgtg cgccggtcgc aacaaccagc
    10141 gccagaatgg ccagactaaa acgtttcata ggacctccgt tcattgtgca ttcctgaata
    10201 agtgtaaacc agtgacacat ttttgaacgg cggctattcc taaaagtgct tacggtacgt
    10261 cgtaccccag tgccgcttta cggatacgaa accattgttg acgggtcatt ttcagtgttt
    10321 ctgcttcgac agctgcccgt acgcgctcaa ttttacctga gccgataatt ggcagcggct
    10381 gcgacggtaa acgtaatacc caggcgtaaa ccacctgttc aatcgagccc gcgtttaact
    10441 cctccgccac cacagccagt tcatcacgca gcggctggaa ataatcatca ttaaacagac
    10501 gaccaccacc aaggcaggac cacgccatcg gacgaacacg cagttgttgt agttggtcga
    10561 gcgtgccatc cagcagtaac ggctgatgca ccggggatat ttccacctga ttagtggcaa
    10621 gggtaaacgg cagacgtgat ttcaacaggg caaattgcgc aggcgtaaag ttcgatacgc
    10681 caaaatgacg cactttgccg ctctgatgca gatgtttgaa cgcgtccgcc acttcatcgg
    10741 catccattaa cgggtctggt cggtggatta acagcagatc cagatgatcg gtcgcgagat
    10801 taattagcga ctgttcggcg ctcttaatga tgtgatcgcg gtcagtgatg taatgaccaa
    10861 tgacgttttc ttcacgcgcg gtcgtcgcga taccgcattt actgacgatt tccatccgtt
    10921 cacgcaggtg aggtgccagt ttcagtgcct cgccaaacgc cgcttcgcac tgatagccac
    10981 cataaatatc agcatggtcc acggtggtca cgccgagatc cagatgctct tcaataaaac
    11041 tgaccagctg gcgggcggac atattccagt ccatcaatcg ccagtagccc atcacaaaac
    11101 gggaaaactc cgggccttgc ggcgcaatag taatacgctg aaccataatc gcttcctctt
    11161 atcagatatg agaggagtat acgcaagatt aggttcaaaa gagtgatggt tgctccggtt
    11221 cgtctgatga cgctggctta tttgcgcgta atttgcgcat taatcgctgc cgacaaaggc
    11281 gcagcacctc ttgtttttcg ccatcgctca ttttattcca gttaaaacgc tcatcccgac
    11341 tacgaaaaca gccgcgacaa aacccgcgtt cgtcagactg gcaaattccc cggcacgggc
    11401 tctggacggg aaagaactct aattgctccg ccacttcgcc ctcctcaaat aagattatta
    11461 ccattattga agctgttaat gtccaaagta gcaactttgc ttgcactaga ccgactggtc
    11521 tactacactc caacgcatga acaaacacac cgaacatgat actcgcgaac atctcctggc
    11581 gacgggcgag caactttgcc tgcaacgtgg gttcaccggg atggggctaa gcgaattact
    11641 aaaaatcgct gaagtgccga aagggtcctt ctatcactac tttcgctcta aagaagcgtt
    11701 tggcgttgct atgcttgagc gccattacgc cacatatcac cagcgactga ctgagttgct
    11761 gcaatccggc gaaggtaact accgcgaccg catactggct tattaccagc aaacactgaa
    11821 ccagttttgc caacatggaa ccatcagtgg ttgcctgaca gtaaaactct ctgccgaagt
    11881 gtgcgatctg tcagaagata tgcgtagcgc gatggataaa ggcgctcgcg gcgtgattgc
    11941 cctgctctct caggcgctgg aaaatggcca tgagaaccac tgtttaacct tttgtggcga
    12001 accgctgcaa caggcacaag tgctttacgc actatggctg ggtgcgaatc tgcaggccaa
    12061 aatttcgcgc aattccgagc cactggaaaa cgcgctggca catgtaaaaa ccattattgc
    12121 gacgcctgcc gtttagcagg cattttttat caccagacga ccgggagcct ttatgtcatc
    12181 tgaaaaactg tattccccac tgaaagtggg cgcaataact gcggcaaacc gtatttttat
    12241 ggcaccgctg acgcgtctgc gcagtattga accgggtgac atccctaccc cgttgatggc
    12301 ggaatactat cgccaacgtg ccagtgccgg tttgattatt agcgaagcca cgcaaatttc
    12361 tgcccaggca aaagggtatg ctggtgcacc tggcatccat agtccggaac aaattgccgc
    12421 atggaaaaaa attaccgctg gcgttcatgc tgaaaatggt catatggccg tacagctgtg
    12481 gcacactgga cgcatttctc acgccagcct gcaacctggc ggtcaggcac cggtagcgcc
    12541 ttcagcactt agcgcgggaa cacgtacttc tctgcgcgat gaaaatggtc aggcgatccg
    12601 tgttgaaaca tccatgccgc gtgcgcttga actggaagag attccaggta tcgtcaatga
    12661 tttccgtcag gccattgcta acgcgcgtga agccggtttt gatctggtag agctccactc
    12721 tgctcacggt tatttgctgc atcagttcct ttccccttct tcaaaccatc gtaccgatca
    12781 gtacggcggc agcgtggaaa atcgcgcacg tttggtactg gaagtggtcg atgccgggat
    12841 tgaagaatgg ggtgccgatc gcattggcat tcgcgtttcg ccaatcggta ctttccagaa
    12901 cacggataac ggcccgaatg aagaagccga tgcactgtat ctgattgaac aactgggtaa
    12961 acgcggcatt gcttatctgc atatgtcaga accagattgg gcgggaggtg aaccgtatac
    13021 tgatgcgttc cgcgaaaaag tacgcgcccg tttccacggt ccgattatcg gcgcaggtgc
    13081 atacacggta gaaaaagccg aaacgctgat tggcaaaggg ttaattgatg cggtggcatt
    13141 tggtcgtgac tggattgcga acccggatct ggtcgcccgc ttgcagcgca gagctgagct
    13201 taacccacag cgtgccgaaa gtttctacgg tggcggcgcg gaaggctata ccgattaccc
    13261 gacgttgtaa tccaacattg cgagcggcgt aaagccgccg ctatactaaa acaacatttt
    13321 gaatctgtta gccattttga ggataaaaag atgcgtcttc ttcataccat gctgcgcgtt
    13381 ggcgatctgc aacgctccat cgatttttat accaaagtgc tgggcatgaa actgctgcgt
    13441 accagcgaaa acccggaata caaatactca ctggcgtttg ttggctacgg cccggaaacc
    13501 gaagaagcgg tgattgaact gacctacaac tggggcgtgg ataaatacga actcggcact
    13561 gcttatggtc acatcgcgct tagcgtagat aacgccgctg aagcgtgcga aaaaatccgt
    13621 caaaacgggg gtaacgtgac ccgtgaagcg ggtccggtaa aaggcggtac tacggttatc
    13681 gcgtttgtgg aagatccgga cggttacaaa attgagttaa tcgaagagaa agacgccggt
    13741 cgcggtctgg gcaactaatc tcctgccggg cgtgaactca tcgcgcccgc atctttactg
    13801 catcgacaag taatatttgt cataatgcgc gctgcaattt atccgtatta agagaatcag
    13861 atgtccgata acgctcaact taccggtctg tgcgaccgtt ttcgtggttt ttatcctgtt
    13921 gtgatcgatg ttgaaacagc cggatttaac gccaaaaccg atgcgctgct tgagattgcc
    13981 gccatcaccc tgaaaatgga tgaacaaggc tggctgatgc cggacaccac attacatttc
    14041 cacgtcgaac catttgtcgg cgcaaatttg cagccagagg ccctcgcctt caacggcatt
    14101 gacccgaacg atcccgatcg cggcgcggtc agcgaatacg aggcgctgca cgaaattttt
    14161 aaagttgtac gtaaaggtat taaagcgagc ggctgtaacc gcgccattat ggtggcgcac
    14221 aatgccaatt ttgatcacag ctttatgatg gccgccgcag aacgcgcctc actgaaacgt
    14281 aacccgttcc accctttcgc cacttttgac actgctgcac tggccgggct ggcactcgga
    14341 caaaccgtat tgtcaaaggc ttgccagacc gctggcatgg acttcgacag cacccaggcg
    14401 cactccgcgc tgtacgacac cgaacgcact gctgtgctgt tttgtgaaat cgtcaaccgc
    14461 tggaaacgtc tgggaggctg gccgctacct gccgccgaag aggtgtaatc gagtcgatgc
    14521 ctgatgacat gcaatgattc aggcatctat agtgaggcta ttccacgcat caagcatgat
    14581 attcacgggg aatagcgtta atggcagata atccagaccc ttcatcgctc ctgccggacg
    14641 tgttttcact ggcgacccgc gactggtttc ttcgcgcctt taaacagccg accgctgtcc
    14701 agtcgcaaac ctggcatgtg gcggcgcgag gcgaacatgc gctggtgatt gcaccgactg
    14761 gctccgggaa aacgctggca gcatttctct acgccctcga tcggctcttc cgcgaaggcg
    14821 gcgaagatac ccgcgaggcg cataagcgta aaacctcacg catcctctat atttcaccga
    14881 taaaagccct gggcaccgac gttcagcgca acttgcagat cccgttgaaa ggtattgccg
    14941 atgaacggcg gcggcgcggc gaaacggaag tcaatcttcg cgtagggatc cgtactggcg
    15001 atacgcctgc acaggaacgc agcaaactca cccgtaatcc gccggatatt ctgatcacca
    15061 cacccgaatc actctatctg atgctgacct cccgcgcgcg cgaaacgcta cgcggcgtcg
    15121 aaacggtaat tattgatgaa gtccacgcgg tagcgggcag taaacgtggt gcgcatctgg
    15181 cgttaagtct ggagcggctc gatgcgctgc tccacacctc agcacagcga attggccttt
    15241 ctgccactgt gcgctcagcc agcgatgtgg cagcatttct tggtggcaat cgcccggtta
    15301 cggtagtcaa cccgcccgca atgcgccatc cgcagatacg aattgtcgta ccggtcgcca
    15361 atatggatga tgtctcatcg gtcgccagca gcaccggcga agacagccat gccggccggg
    15421 aaggctccat ctggccatat attgaaacgg gtatccttga tgaagtgttg cgccatcgct
    15481 cgaccattgt ctttactaat tcacgtgggc tggcggaaaa actgacggca cgattaaatg
    15541 agctttacgc cgcacgctta cagcgttccc cgtctatcgc cgttgatgcg gcccatttcg
    15601 agtcgacctc cggcgcaacc tctaaccgtg tacaaagtag cgacgttttt attgcccgct
    15661 cacaccacgg ctccgtctct aaagaacaac gagcaatcac cgagcaggcg ctgaaatcgg
    15721 gtgagttacg ctgcgtggtc gcaacctcca gtctcgaact ggggattgat atgggcgcag
    15781 tggatctgat gattcaggtg gcaacgccgc tttctgttgc cagtgggtta caacgcattg
    15841 gtcgcgccgg acatcaggtt ggcggtgtat ctaaagggct gtttttccct cgtacccggc
    15901 gtgacttagt cgattccgca gtcattgtag agtgtatgtt cgcaggcagg ctggaaaacc
    15961 tgacaccgcc gcataatcca ctcgacgtcc ttgcgcagca aaccgttgcc gccgcggcga
    16021 tggatgcatt acaggtagac gaatggtact cccgcgtacg ccgtgccgca ccgtggaaag
    16081 atctgccaag acgtgttttt gacgctacgc tggatatgct ttccgggcgc tatccctctg
    16141 gcgatttttc tgcttttcgc cccaaactgg tctggaaccg ggagaccggg atattgaccg
    16201 cccgacctgg cgctcaattg ttggcggtta ccagcggtag caccattccg gatcgtggca
    16261 tgtatagcgt gttattaccc gaaggtgaag aacaggccgg ttcgcggcgg gtgggtgaac
    16321 tggatgagga gatggtatat gagtcgcggg tgaacgacat tatcactctc ggcgctacct
    16381 catggcggat ccagcaaatc acccgcgatc aggtgattgt gactcctgct ccgggtcgtt
    16441 ctgcccggct ccccttctgg cgtggtgaag gtaacggacg tccggctgaa ttaggcgaga
    16501 tgatcggcga ttttcttcat ttgctggcgg atggcgcgtt cttttccggg actattcccc
    16561 cgtggctggc agaagaaaat acgatcgcca atattcaggg gttgattgac gagcaacgca
    16621 acgcgacggg catcgttccg gggagtcgcc atctggtcct cgaacggtgc cgtgatgaaa
    16681 ttggcgactg gcgtattatt ttgcactctc cctatggaag acgggtgcat gaaccctggg
    16741 cgctggcgat tgccgggcga atacatgccc tatggggcgc tgacgcgtct gtggtcgcca
    16801 gtgatgacgg cattgttgcg cgcattcctg acactgatgg taaattgccc gacgccgcga
    16861 tttttttgtt tgaaccagaa aagttgctgc aaattgtccg cgaggcggta ggcagctcgg
    16921 cacttttcgc cgcccgtttt cgcgaatgcg ccgcgcgggc attattaatg ccggggcgca
    16981 ctccgggcca tcgcaccccg ctttggcaac aacggctgcg cgccagtcag ttgctggaaa
    17041 tcgctcaggg atatccggat tttccggtca ttctcgaaac cctacgcgaa tgtctgcaag
    17101 atgtttatga tcttcccgca ctggaacgtt tgatgcgtcg cctgaacggt ggcgaaattc
    17161 aaatatccga tgtaacgacc actacgccct cgcctttcgc cacaagttta ttgttcggct
    17221 atgtcgcgga atttatgtac cagagcgacg ccccgctggc agagcgccgg gcatccgtac
    17281 tgtcgctgga cagcgagtta ctgcgcaatc tactcggaca ggtcgatccg ggggaattac
    17341 tcgacccgca ggtcattcgc caggtggaag aagagttgca acgactggct cctggcagaa
    17401 gagcgaaagg tgaagaagga ttgttcgacc tgctgcgcga actggggcca atgaccgttg
    17461 aagacctggc gcaacggcat acaggcagca gtgaagaggt tgcgtcgtat ctggaaaatc
    17521 ttcttgcagt aaaacgaatc ttcccagcga tgattagcgg acaggagcgt cttgcctgta
    17581 tggatgatgc cgccaggctg cgtgatgccc tcggcgtacg actaccagag tcattgccag
    17641 agatttattt acatagagtc agttacccgc ttcgcgacct ctttctgcgc tatctccggg
    17701 ctcatgctct ggtcacggct gaacaactgg ctcatgagtt tagtctcggt attgccattg
    17761 tcgaagagca gcttcagcaa ctgcgtgaac agggtctggt gatgaatctg caacaagaca
    17821 tctgggtgag cgatgaagta tttcgtcgtc tgcgtttgcg ctcgctgcaa gccgccagag
    17881 aagcgacgcg tcccgttgca gccacgacct atgcgcgatt gctgctggaa cgtcagggcg
    17941 tattacccgc caccgatggt agcccggcgc tctttgcctc aacatcgcca ggcgtttatg
    18001 agggcgtaga tggcgtgatg cgggtgatcg aacagcttgc cggagtcggt ttacccgcct
    18061 cactctggga aagccagatc ctgcctgccc gcgtacgcga ctattcgtca gaaatgctcg
    18121 atgaattact ggcaaccggt gcggttatct ggtcggggca aaaaaactgg gtgaagatga
    18181 cggcctggtg gcactgcatc tacaggaata tgctgcagaa tcgttcactc ccgccgaagc
    18241 ggatcaggcg aatcgttcgg cgctgcaaca agcgatagtc gctgttctgg ctgacggagg
    18301 agcctggttt gcacaacaaa tcagccaacg gatacgcgac aaaatcggcg aatcggttga
    18361 tctctctgcc ctgcaagagg cgttatgggc gctggtctgg caaggcgtca tcaccagcga
    18421 catttgggca ccgttacgcg ccctcacccg cagcagttcc aacgcacgca cctcaactcg
    18481 ccgcagtcac cgggctcgtc gtggacgtcc tgtctatgcg caacccgtct cgccgctggt
    18541 atcttacaac acaccaaatc tggctggatg ctggcgctgg cggaaaatat gctcgaccgc
    18601 tacggcatca tcagtcgtca ggcggtgata gccgaaaata tccctggcgg gtttccatcg
    18661 atgcaaacgc tttgtcgaag tatggaagac tccgggcgaa ttatgcaagg tcgttttgta
    18721 gaaggtctgg gtggcgcgca attcgctgaa cgtctgacta ttgaccgatt gcgcgatctg
    18781 gcgacacaag ccacgcaaac gcgccactat acaccagtgg cgctctctgc caacgatccg
    18841 gctaatgtgt ggggaaatct tctgccctgg cctgcacatc cggcaacgct ggttccaacg
    18901 cgtcgggcgg gtgcgctggt ggtcgtttct ggcggcaaat tgttactcta tctggcgcaa
    18961 ggtggcaaaa aaatgctggt ctggcaggaa aaagaggaat tactcgcccc agaggttttc
    19021 cacgcgctga ctaccgcact gcgtcgcgaa ccacggctgc gctttacgct aacagaagtg
    19081 aatgatctac cggtccggca aacgccgatg tttacgctgc tgcgtgaggc gggattttca
    19141 agttcgccac aagggctgga ttggggatag agaaaggact gacggatgcc cgttcgcatc
    19201 cgtcagtatt gcaggacgga ttattccgcg tccggctctt cagacttgta tttagcggca
    19261 gtttctttga tcagctgctg cagttcgcca cgctgataca tttcgatcac gatatcacaa
    19321 ccgccgacca gcttgccgtc aacccacagt tgcgggaagg tcggccagtt agcatatttc
    19381 ggcagttccg cacgaatgtc cggattctgc agaatatcaa cataggcaaa acgttcgcca
    19441 catgcggcaa gcgcctggac tgcctgggca gagaaaccgc agctcggcag tttcggtgaa
    19501 cctttcatgt acagcaggat cgggttttca gcaatctggc gttggatttt ttcgatagtg
    19561 gtgctcattg tcttgcttcc tcaaacttct ttacggcagt aatctgacat tgtagcgggt
    19621 cagtgcggca tcggaaaata acattttcat cacgcttttg ctattttatc cctttgctct
    19681 atttgttgca tttcaaatat tcgtttcttc tctgatgccg gcaaactggc gtatttataa
    19741 ccatttttag ctttcactgc tgcatttttt tgcgctcgcc aacgaaacgt attttttaac
    19801 aataaaagct attaactttc tcttcttcta tgcattagaa tcatcaagtt ttgtaaatca
    19861 gacgcaggca tgatagacct gccttaacag agggacgctc agtggcgcgg ataaaccgta
    19921 tttcgatcac gctctgtgct ttacttttta ccaccctgcc tttaacgcct atggcccatg
    19981 cttcaaagca agccagggag agttctgcta ccactcatat caccaaaaaa gcagataaaa
    20041 agaaaagcac ggcaaccacc aaaaagaccc agaaaacagc gaaaaaagcc gccagtaaaa
    20101 gtacgaccaa aagcaaaacc gcttcttccg ttaaaaaatc ttccattacc gcttctaaaa
    20161 acgccaaaac tcgcagcaaa cacgccgtca ataaaacggc ctcagccagc tttaccgaaa
    20221 agtgtaccaa acgtaagggc tataaatcgc attgtgtgaa agtcaaaaat gccgcgtcag
    20281 gaactcttgc cgacgcgcac aaagcgaagg tgcaaaaagc tacgaaagtg gcaatgaata
    20341 aactgatgca gcaaattggt aagccatatc gttggggtgg cagctcaccg cgtaccggtt
    20401 ttgattgcag cggcctggtt tattacgctt ataaagattt ggtgaaaatt cgtattccgc
    20461 gtacggcgaa tgaaatgtat cacctgcgtg atgcagcgcc aatcgaacgt agtgaactga
    20521 aaaacggcga cctggtcttt ttccgtactc agggacgcgg cacagccgat catgtcggcg
    20581 tgtatgtcgg caacggcaaa tttattcagt caccgcgcac aggtcaggaa attcaaatca
    20641 cttctctcag tgaagactac tggcagcgcc actatgttgg cgctcgtcgg gtaatgaccc
    20701 caaaaacact tcgctaaaac tttaccctgt tgttacggca acagggtaag ttcatctttt
    20761 gtctcacctt ttaatttgct accctatcca tacgcacaat aaggctattg tacgtatgca
    20821 aattaataat aaaggagagt agcaatgtca ttcgaattac ctgcactacc atatgctaaa
    20881 gatgctctgg caccgcacat ttctgcggaa actatcgagt atcactacgg caagcatcat
    20941 cagacttatg tcactaacct gaacaacctg attaaaggta ccgcgtttga aggtaaatca
    21001 ctggaagaga ttattcgcag ctctgaaggt ggcgtattca acaacgcagc tcaggtctgg
    21061 aaccatactt tctactggaa ctgcctggca ccgaacgccg gtggcgaacc gactggaaaa
    21121 gtcgctgaag ctatcgccgc atcttttggc agctttgccg atttcaaagc gcagtttact
    21181 gatgcagcga tcaaaaactt tggttctggc tggacctggc tggtgaaaaa cagcgatggc
    21241 aaactggcta tcgtttcaac ctctaacgcg ggtactccgc tgaccaccga tgcgactccg
    21301 ctgctgaccg ttgatgtctg ggaacacgct tattacatcg actatcgcaa tgcacgtcct
    21361 ggctatctgg agcacttctg ggcgctggtg aactgggaat tcgtagcgaa aaatctcgct
    21421 gcataataac tgatggcaaa tgcagcattg cctgatgcgc ttcgcttacc atgccaacgc
    21481 ggatgaggcg ttaacagcgc attcggcaag ataaatcgca cgttgtcagc aactgtaacg
    21541 cagaaggtta tccttctgcg tttttgttta attagctgtt ggcaacgcaa actgtttcag
    21601 gttgttttct ggctgacata aacaccagca ataatgccag tcccgcgaca atcgccccca
    21661 tcaccggcac aaagctgtat cccagcccag cggaaattac cgcaccacca gccgctgctc
    21721 ccagcgcatt tccaagatta aaggcaccaa tattgactga tgaagacaga cctggcgctt
    21781 cactggcgac acgcatcacg cgcatctgta acggcggtac gaccgcaaag gttgctgcgc
    21841 cccacaccac catgctaata gctgcgccga actcattgcg ggccaggaac ggggttgcca
    21901 gcataatcac catcagcagc aacaaaaagc ctttcaacgt gccgttaact gaacgatctg
    21961 ccagtttgcc gccgagatag ttaccgatag agaatccgac accaatcagc accagcattg
    22021 ccgtgacgaa caccggtgtt gcgtgggtaa tactttgcag taccggagag atataggtgt
    22081 agagagtaaa cattgcgcca gctcccagta ccgtcgtcag caatgcagac agcacctgcg
    22141 gacgcattaa taccgccagc tcttttttca cttcaggtcg tgcccctgta ccacctttag
    22201 gtaatgagaa gaacagactt accattgaaa tcactcccag ccccgccgtt gccagaaatg
    22261 acatccgcca gccgatggtt tcacccaacc aggtcgccgc cggcacgcca ccgatatttg
    22321 ccagggttaa ccccataaac atagtggcaa ctgcgctggc ctgtttatgt tttggcacca
    22381 cgcttgcggc cacgactgaa cccaaaccaa aaaatgctcc gtgattcagg ctggtcaaaa
    22441 tgcgtgaaag catcagggtc atataatccg gcgcgatggc ggaaagtacg ttgccgagcg
    22501 tgaaaattgc catcaggaaa atcaacgcac tgcggcgggc acgatgagaa agtagaagcg
    22561 tcatcagcgg cgcgcccacc attacgccaa ctgcataggc actgattaac attccggcag
    22621 cgggaatcga gacatccaca ccgcgcgcaa tgacgggcaa caagcccatt ggcgagaact
    22681 ccgttgtccc gataccaaac gcgccaatcg ccagcgccag caacggatag ttaattttca
    22741 tgccttatct ccacctcttc gcgtcattac gcgatattca ttaaagtggc gaaagcatga
    22801 cagcaatcac aaaaaaatga aaataacaaa aagagaaaac acttttgcca ttttgctaac
    22861 aaacaggaag gagatgcgag ggagaacgcg ctccctcgag aggaaatcag tgcagcgcgg
    22921 cagtcaaacc cacggctacg atcaaaccga ggacgataat cgttgttacc agtgaaaatt
    22981 taaggtcggt gctcatcaag ttttctcctt ttttattacc acacaaaaag tgatattacg
    23041 catttttaca cactgtgatg aaaaaatctc ccgtcattta taatgataag tgtttttacc
    23101 acttcccctt ttcgtcaaga tcggccaaaa ttccacgctt acactatttg cgtactggcc
    23161 attgacccct tcctgacgct ccgtgtcgtt tttccggcgt accgcaacac ttttgttgtg
    23221 cgtaaggtgt gtaaaggcaa acgtttacct tgcgattttg caggagctga agttagggtc
    23281 tggagtgaaa tggaatggca acaataaaag atgtagcgaa acgagcaaac gtttccacta
    23341 caactgtgtc acacgtgatc aacaaaacac gtttcgtcgc tgaagaaacg cgcaacgccg
    23401 tgtgggcagc gattaaagaa ttacactact cccctagcgc ggtggcgcgt agcctgaagg
    23461 ttaaccacac caagtctatc ggtttgctgg cgaccagcag cgaagcggcc tattttgccg
    23521 agatcattga agcagttgaa aaaaattgct tccagaaagg ttataccctg attctgggca
    23581 atgcgtggaa caatcttgag aaacagcggg cttatctgtc gatgatggcg caaaaacgcg
    23641 tcgatggtct gctggtgatg tgttctgagt acccagagcc gttgctggcg atgctggaag
    23701 agtatcgcca tatcccaatg gtggtgatgg actggggtga agcaaaagct gacttcaccg
    23761 atgcggtcat tgataacgcg ttcgaaggcg gctacatggc cgggcgttat ctgattgaac
    23821 gcggtcaccg cgaaatcggc gttatccccg gcccgctgga acgtaacact ggcgcaggcc
    23881 gccttgtcgg ttttatgaag gcgatggaag aagcaatgat caaggtgccg gaaagctgga
    23941 ttgtgcaggg tgactttgaa cctgaatccg gttatcgcgc catgcagcaa atcctgtcgc
    24001 agccgcatcg ccctactgcc gtcttctgtg gtggcgatat catggcaatg ggcgcacttt
    24061 gtgctgctga tgaaatgggg ctgcgcgtcc cgcaggatgt ttcgctaatt ggttatgata
    24121 acgtgcgcaa cgcccgctat tttacgccgg cgctgaccac aatccatcag ccaaaagatt
    24181 cgctgggtga aacagcgttc aacatgctgt tggatcgtat cgtcaacaaa cgtgaagaac
    24241 cgcagtccat tgaagtgcat ccgcgcttga ttgaacgccg ctccgtggct gacggcccgt
    24301 tccgcgacta tcgtcgttaa tcacccgttg cgggagtctc ttccggctcc cgcagccact
    24361 ccttattcag cgtctcacta tcgccgagat actcaagcaa ccaggttaac gcaggcgaca
    24421 tatcattttg ctgccatgtc agacaacatg ccgaatccgg aaaggggttt tccagttcaa
    24481 gtgctaccca cttcccctca ttaagccacg gtttggcgaa atgtgttggc accatcccta
    24541 tgcataatcc tgccgagata caggttgccg atgattccca gtcaggcacg acaactcttt
    24601 tttggttatc cagcaaccag gtaatacgtt taggtagcgt tcgcgaggtg tcttcgcgca
    24661 ccaacgacgg ccagttgcgc aacgtatcat cgctgaacgg gccatccatc aacgccagcg
    24721 ggtgatggct ggcaacaacg caactccagc ttagcatccc catatcccgg aaggcataac
    24781 gaccgcctac cggaatcgcg cgtgttgcgc caatcgccag ttccacgcgc ccgtcggaaa
    24841 gcgcatccca gacaccgttg aacacttcct gaaagacaag aagttcgaca tcatcaaaat
    24901 ggcgataaaa atcaacgatc atctgccgtg tacgttctgg cctgacaata ttatccactg
    24961 cgatagctaa ctgaccgcgc cagccgttcg ctatctgctg acattgctgg cgggtgatct
    25021 gcattttttt gacaacagag cgcccttctt tgagaaacca cgctccagca gcggtcagct
    25081 ccacatcacg gtgccgtcgt tcaaagagcg gcaccgccag ccactcttcc agctgacgca
    25141 cggtatagct gaccgcagaa ggaacgcgat gcagctcctg tgccgcagcg ctaaaactac
    25201 cattacgcgc taccgcatca acaacttcga gtgaatattc tgaccacata gtctgcctgc
    25261 aaaatttttg aaaccaatca tcaaatatta ccgtttcaca acactaattt cactccctac
    25321 actttgcggc ggtgtttaat tgagagattt agagaatata catgcaacct gggaaaagat
    25381 ttttagtctg gctggcgggt ttgagcgtac tcggttttct ggcaaccgat atgtatctgc
    25441 ctgctttcgc cgccatacag gccgacctgc aaacgcctgc gtctgctgtc agtgccagcc
    25501 ttagtctgtt ccttgccggt tttgccgcag cccagcttct gtgggggccg ctctccgacc
    25561 gttatggtcg taaaccggta ttattaatcg gtatgacaat ttttgcgtta ggtagtctgg
    25621 ggatgctgtg ggtagaaaac gccgctacgc tgctggtatt gcgttttgta caggctgtgg
    25681 gtgtctgcgc cgcggcggtt atctggcaag cgttagtgac ggattattat ccttcacaga
    25741 aagttaaccg aatttttgcg accatcatac cgctggtggg tctatctccg gccctggctc
    25801 ctctgttagg aagctggctg ctggtccatt tttcctggca ggcgattttc gccaccctgt
    25861 ttgccattac cgtggtgctg attctgccta ttttctggct caaacccacg acgaaggccc
    25921 gtaacaatag tcaggatggt ctgaccttta ccgacctgct acgttctaaa acctatcgcg
    25981 gcaacgtgct gatatacgcg gcctgttcag ccagtttttt tgcatggctg accggttcac
    26041 cgttcatcct tagtgaaatg ggctacagcc cggcagttat tggtttaagt tatgtcccgc
    26101 aaactatcgc gtttctgatt ggtggttatg gctgtcgcgc cgcgctgcag aaatggcaag
    26161 gcaagcagtt attaccgtgg ttgctggtgc tgtttgctgt cagcgtcatt gcgacctggg
    26221 ctgcgggctt cattagccat gtgtcgctgg tcgaaatcct gatcccattc tgtgtgatgg
    26281 cgattgccaa tggcgcgatc taccctattg ttgtcgccca ggcgctgcgt cccttcccac
    26341 acgcaactgg tcgcgccgca gcgttgcaga acactctaca actgggtctg tgcttcctcg
    26401 caagtctggt agtttcctgg cttattagta tcagcacgcc attgctcacc accaccagcg
    26461 tgatgttatc aacagtagtg ctggtcgcgc tgggttacat gatgcaacgt tgtgaagaag
    26521 ctggctgcca gaatcatggc aatgccgaag tcgctcatag cgaatcacac tgatctatat
    26581 cgatatactt atacttaggc tgctaacaaa attttgttgt atgattgaaa ttagcggcct
    26641 atactaattt cgagttgtta aagctacgat aaatattatg tttttacggg gacaggatcg
    26701 ttcccgactc actatggatg gtcatttcgg caagggttcc tcctttccct ctgttctacg
    26761 tcggattata gactcgcggt tttttctgcg agatttctca caaagcccaa aaagcgtcta
    26821 cgctgtttta aggttctgat caccgaccag tgatggagaa actatgagtt catcgtgtat
    26881 agaagaagtc agtgtaccgg atgacaactg gtaccgtatc gccaacgaat tacttagccg
    26941 tgccggtata gccattaacg gttctgcccc ggcggatatt cgtgtgaaaa accccgattt
    27001 ttttaaacgc gttctgcaag aaggctcttt ggggttaggc gaaagttata tggatggctg
    27061 gtgggaatgt gaccgactgg atatgttttt tagcaaagtc ttacgcgcag gtctcgagaa
    27121 ccaactcccc catcatttca aagacacgct gcgtattgcc ggcgctcgtc tcttcaatct
    27181 gcagagtaaa aaacgtgcct ggatagtcgg caaggagcat tacgatttgg gtaatgactt
    27241 gttcagccgc atgcttgatc ccttcatgca atattcctgc gcttactgga aagatgccga
    27301 taatctggaa tctgcccagc aggcgaagct caaaatgatt tgtgaaaaat tgcagttaaa
    27361 acctgggatg cgcgtactgg atattggctg cggctggggc ggactggcac actacatggc
    27421 atctaattat gacgtaagcg tggtgggcgt caccatttct gccgaacagc aaaaaatggc
    27481 tcaggaacgc tgtgaaggcc tggatgtcac cattttgctg caagattatc gtgacctgaa
    27541 cgaccagttt gatcgtattg tttctgtggg gatgttcgag cacgtcggac cgaaaaatta
    27601 cgatacctat tttgcggtgg tggatcgtaa tttgaaaccg gaaggcatat tcctgctcca
    27661 tactatcggt tcgaaaaaaa ccgatctgaa tgttgatccc tggattaata aatatatttt
    27721 tccgaacggt tgcctgccct ctgtacgcca gattgctcag tccagcgaac cccactttgt
    27781 gatggaagac tggcataact tcggtgctga ttacgatact acgttgatgg cgtggtatga
    27841 acgattcctc gccgcatggc cagaaattgc ggataactac agtgaacgct ttaaacggat
    27901 gtttacctat tatctgaatg cctgcgcagg tgctttccgc gcccgtgata ttcagctctg
    27961 gcaggtcgtg ttctcacgcg gtgttgaaaa cggccttcga gtcgctcgct aaaggctatt
    28021 ctatcgcccc ctctccgggg gcgatttcag atcaggcttc tgtacctggt tgattcatgg
    28081 cattttctcg tgccgccagc acacgttcta ccgtatctac cactgcctga gtttgtggat
    28141 caatttcaat gttgacgcgt gcgccgagtt ttttcttccc aagagtcgtg cgttccagag
    28201 tttccggaat taaatggacg caaaaacgcg ttggcgtgac ttcgccgacg gtcaggctaa
    28261 taccgtcgat gccaataaat cctttgtaca ggatatattt catcaactga ctatcctgga
    28321 ctttaaacca gatctggcga ttattttccg aggttaatat tttcgccact tcagcggtgg
    28381 tcataatatg acctgacatt aagtgtccgc caatttcatc gctgaatttt gccgcacgct
    28441 caacgtttac ccaatccccc acttttaaat cgccaagatt ggtaatgcgt aacgtttctt
    28501 tcatcaggtc aaaactgaca tggttgccgt taatttccgt cactgtcagg cagcaaccgt
    28561 tatgcgccac ggaagcacct gtttccaggc cgtccagcat gtggtcgggt aactccacca
    28621 catgcgtacg aaaatttggt ttctcgtcaa tcgacaccag ttttgcggtg ccctgtacaa
    28681 tccccgtaaa catacttaca actcctgaaa tcagttaaga cattctgttc agcacaatag
    28741 caggtggaaa acgcccttac cagtgaaggg gtaagaatga ctattttttc actggagaat
    28801 taataaatcc tcgctacaat agactgaatt tcccctgctt cttctttttg ctgcccattc
    28861 aggcggcttt ttagtctctc atataactac aaataaaagg tgttcacgtg cagaagtata
    28921 tcagtgaagc gcgtctgtta ttagcattag caatcccggt gattctcgcg caaatcgccc
    28981 aaactgcgat gggttttgtc gataccgtga tggcgggcgg ctatagtgcc accgacatgg
    29041 cggcggtcgc tatcggtact tctatctggc ttccggcgat cctctttggt cacggactgc
    29101 tgctggcatt aacgccggtt atcgcgcaat taaatggttc cggtcgacgt gagcgcattg
    29161 cgcatcaggt gcgacaaggt ttctggctgg caggttttgt ttccgttctc attatgctgg
    29221 tgctgtggaa tgcaggttac attatccgct ccatggaaaa catcgatccg gctctggcgg
    29281 acaaagccgt gggttatctg cgtgcgttgt tgtggggcgc gccaggctat cttttcttcc
    29341 aggttgcccg taaccagtgt gaaggtctgg caaaaaccaa gccgggtatg gtaatgggct
    29401 ttatcggcct gctggtgaac attccggtga actatatctt tatttatggt catttcggta
    29461 tgcctgagct cggtggcgtt ggttgtggcg tggctactgc ggcggtgtat tgggttatgt
    29521 tccttgccat ggtttcttac attaaacgcg cccgctccat gcgcgatatc cgtaacgaaa
    29581 aaggcaccgc aaaacccgat cctgcggtta tgaaacgact gattcaactc ggtttgccga
    29641 ttgcgctggc actattcttt gaagtgacac tgtttgccgt cgtggctctg ttagtgtctc
    29701 cgctcggtat tgttgatgtc gcaggacacc agattgccct gaactttagt tcactaatgt
    29761 tcgtgcttcc aatgtcgctg gcggcagcgg taactatccg cgtaggttat cgtctgggtc
    29821 agggctcaac gctggatgcg caaaccgctg ctcggaccgg gcttatggtg ggtgtctgta
    29881 tggcaaccct gacggccatt ttcacggttt cactgcggga gcaaatcgcc ctgttgtaca
    29941 acgacaatcc cgaggttgta acgctggctg cgcatttgat gttgctggcg gcggtatatc
    30001 agatttctga ctcaatccag gtgattggca gtgggatttt gcgtggttat aaagatacgc
    30061 gttccatttt ctatatcacc tttacagctt actgggtgct gggcttgcca agcggctata
    30121 ttctggcact gaccgatctg gtcgttgaac ctatggggcc agcaggcttc tggataggct
    30181 ttattatcgg cctgacatcg gcagccatta tgatgatgct gcgtatgcgg ttcctgcaac
    30241 gtatgccgtc agccatcatt ctgcaacgag cgtcccgcta ataaagacaa ggcgcaacct
    30301 tcacgggttg cgcctgtgtt tttacgcagg ctggagcgtt gcgccaatcc cgtcttcgtc
    30361 tggctgtaat ttcagagcgt tacacagagt ttgccgactg gcatctgtta tctcaacgcg
    30421 tatatccccg ttaacttccc ctttcacatt gatgaagtca ggtgaaattt ctttcgcact
    30481 ggtttgcagg caaatcgctc cctctccgcg caggcgtttc actttcagtt gcacatgcgt
    30541 tgccgcacca gtgatcagcc acagttttcc gccattcaga ttgatgttac caacaatgaa
    30601 agtgacgctg tcacctttta ccgtactgcc gtcgagaatc aacacaccgc cctgctgtac
    30661 agtaatcgtt gattgttgca gctctgcgcc ataaccaata atcattgaag cgccgtccag
    30721 taatgcaaca ctcccggtca gttcaaccgg tgcgcgatct tcctctacga caaaattttc
    30781 gtccgcgctg gtgggtgaca acaggatcac tgttccacct tgtattaccg cattttccag
    30841 cgtaccggca tgcacttcag cccgcccccc cacattcacc gacaggtttt gcgtcttacc
    30901 ggaactgtag agctgaaggc catccgtccc cagacgataa atggatccat tctcaacaat
    30961 agtgtcagag tcttcaccac cgagattatt catggtgcca ccagcaagca acgtatcact
    31021 ggctttcccg gccagcataa aaacgccgcc ctgattaatc tcacaattgc tggcttctcc
    31081 acttggatac accaacaatt caccaccttc atctaccacg accgctgagg ttgtcccatt
    31141 aaccaacagg ccgccctctt gatcgagaat gattttttcc gcgcgatgtc cttccagtac
    31201 acgcaggtta ccgccatttt ccagcaacaa accgcaggca taaccctgat caacgctgaa
    31261 ttcaccttcg ggatggcggc cattaaccgt agcgagcgta gagagactaa ttgcgcctcc
    31321 ctcggactga acaatatctg tggcaatggc accgtcgctg accgatagcg tgccattgtt
    31381 accgtactgt gtgcatttgg cgatcccacc ggttgccact ttcaaccagc cagcccccgt
    31441 gaccacggta ttttcggctc ttccgccagc ttcaacaatc aactcgcctt cagtaagttg
    31501 cgtcgcgcca atgacctcgc cggtcttaac aatttgcaca ttcccgtcgc tcatcaagtt
    31561 tttcgcatca gatcccatga tttattcctt tgctgcatct gtgtgccttt attgctacct
    31621 aagtgtaaag gctacggagg atttatccac gacagatttg agatggtggc aaacaattct
    31681 gtttaaactc tgatacacga attattgggt tgtatcagat gtaaatgcga tcctgaataa
    31741 aaatcgccct tgcaaatcaa caaaatatag ccaatttgct taaaagctct gcaaacggtc
    31801 gaaatagcgc agaaaattac gttttgcctc ttgccacctt tccactctgc cgctaatatt
    31861 tgtccccgtt gtcacctaca acgttgcgtt catagctcag ttggttagag caccaccttg
    31921 acatggtggg ggtcgttggt tcgagtccaa ttgaacgcac catcctgcgt ccgtagctca
    31981 gttggttaga gcaccacctt gacatggtgg gggtcggtgg ttcgagtcca ctcggacgca
    32041 ccagattttc ttaatctggt cttttccttt ttccctctgt ttattctctg tatccaatac
    32101 gttaaaagat ttacactgtc ttcgtatgcg ttatcagaag gagaatcgct atggcaactt
    32161 tgttacaact tcattttgct tttaatggcc catttggcga cgcaatggct gagcagctta
    32221 aaccacttgc tgagtcgatt aatcaggaac ctggttttct gtggaaggta tggacagaaa
    32281 gtgaaaagaa ccacgaagcc ggtgggatct accttttcac tgatgaaaaa agcgcccttg
    32341 cctatctgga aaaacatact gcccgactga aaaatctcgg cgttgaggaa gttgtcgcca
    32401 aagttttcga tgtcaatgag ccacttagtc aaatcaatca ggcaaaactc gcctgacaga
    32461 atttaatcaa gggcggttag cgcccttttc atccctgtct gaaatttctc aaattctaaa
    32521 aatctcaacc aaacttatct gataacacta aattcgaaag aatgcgtaca ggtaagtaac
    32581 aatgaaaaaa attgctattg tgggtgccgg gcctacgggg atctacacct tattctcgct
    32641 tctacagcaa caaactccac tttctatttc tatcttcgag caggctgacg aggccggtgt
    32701 cgggatgcca tacagtgatg aggaaaactc aaaaatgatg ctggcaaata ttgccagtat
    32761 tgaaataccg ccgattaatt gtacgtatct cgaatggcta caaaagcaag aagccagcca
    32821 tctccagcgt tatggcgtta aaaaagaaac cttgcacgat cgtcagtttt taccgcgaat
    32881 tctgctgggc gaatatttcc gcgatcaatt tttacgatta gtagaccagg cacgacagca
    32941 aaaatttgca gtggctgttt atgaatcatg ccaggttacc gatctgcaaa ttacaaatgc
    33001 tggcgtcatg ctcgctacaa atcaggattt acccagcgag acgtttgatt tagcggtgat
    33061 tgccacgggt cacgtctggc ctgatgaaga agaagcaacc agaacgtatt ttcccagccc
    33121 gtggtcaggc ctgatggaag caaaggtcga tgcgtgtaac gtgggtatta tgggaacatc
    33181 cttgagcgga ctggatgcgg caatggcagt ggctattcag catggttcgt tcattgaaga
    33241 tgataaacaa cacgtcgttt ttcaccgcga taacgcaagt gaaaagctaa atatcacgtt
    33301 gatgtcgcgc acgggtattt tacccgaagc cgatttctat tgccctattc cctacgagcc
    33361 cttacacatc gtcaccgatc aggcattaaa tgctgagatt caaaaaggcg aatatggcct
    33421 tttggatcgg gtatttagat tgatagtaga ggaaatcaag tttgctgatc cagactggag
    33481 ccaacgcata gccttagaga gcctgaatgt cgattccttt gctcaagcct ggtttgccga
    33541 gcgcaaacaa cgcgacccat ttgactgggc agaaaaaaat ctccaggaag tcgaacgcaa
    33601 taaacgagaa aaacatactg ttccctggcg ttatgtcatt ctgcgcctgc atgaagccgt
    33661 acaggaaatt gttccacatc tgaatgaaca cgaccataaa cggttcagta aaggccttgc
    33721 ccgggttttc atcgataatt atgcggcaat cccttcagag tctattcgtc ggctgctggc
    33781 cttacgtgaa gcggggatca ttcatattct cgccctcggt gaagactaca aaatggaaat
    33841 taatgagtcg cgcaccgtcc tgaaaacgga agacaacagc tactcgtttg acgtttttat
    33901 tgatgcccgc ggacagcgtc cgcttaaagt gaaagatatt cctttccctg ggctacgcga
    33961 gcaattacag aaaacagggg atgaaatccc tgatgttggc gaagattata cgttacagca
    34021 acccgaagat attcgtggac gcgtagcgtt cggcgcgttg ccctggttga tgcacgacca
    34081 gcctttcgtt cagggactta cggcatgtgc agaaattggt gaggcgatgg ctcgggcggt
    34141 cgtaaagcct gcatcccgtg ctcgtcggcg tctttcgttt gattaaaggt aaagctatca
    34201 gcactcaaca aactgatgct gttcattaag acgatagcgg actccggttc gaatattgtt
    34261 ttcaccttca atggcgacgg cgaagcgaac tctttcgccg tcatgatatg ccagtgcggc
    34321 gcttccccct ggcccgagaa taattgagtc gaccacgccc gtgctcacta ccgttgaatt
    34381 ttcaccgctg gcaatgatgt gtacattgtc gccgctgttg gcaattctgg cgtttgcgcc
    34441 aaagctggcg atttgcacca ggtcaccatt actggcgaca tgacaccgtt cacccaatgt
    34501 acaaacgcgt actcgcattc cggtattcgc aatccgacta ctatctccag cgctgcttat
    34561 ccgcgaagaa ttaccggtta cagcaacccg tgcgcgttca ccggagctgc caatgtgact
    34621 gttaaagccg acacttccaa tccgcacgct ataacccata ctggcaattt gcgcggcata
    34681 cccagcggtt acgattcggg cgttatcctc acaacagccg atgcgagcat gatcgtcgct
    34741 gcgggcaaac tgactgcaaa atggtatctc tccggggtct gtggccagtt tttgcataga
    34801 ggaaacttcc tgatgcgcga agttttcatc cgccagccat tttttccacg catactcaac
    34861 cagactctcc gcccagtccg tatatccttc ctcaatcagt gcatgatgaa tatcggcata
    34921 actaccgccg cgtgggaaat ggcgcaggaa ccagcgatac atcaccgctc cgatacgcca
    34981 ttctcgcagg tctgctcgtg taatgatcat tcgttagtgc cggtgatagc catcgaccat
    35041 gcttttaaag gtttcgtgtg gcgtacgccc cgtggtgcaa agataaagat gtccgaagat
    35101 aaaaaagagg cttataaatg ccagagcaaa atgcgcctgc aataaccagt atcttacgcc
    35161 aggaaacaca tctcccacgg cttgcggata gagacacaac agccccgtca atagtaacaa
    35221 cggcagcaat ccacacatga caccaacata ggcgacctgc tgtaagggat taaatttaga
    35281 ctgtgttgtt gccgggaaag gatgttcttc cccctgcata atgccaaaca aataaaatcg
    35341 tgtttgtttt gccgctcgtt ccagccaccc ctgacgacga atgcgatagt ggtgaccatt
    35401 atccccaacg gcattgatca gcacaaagcc gagccagcat gccagtaaca aaaatccgca
    35461 aacttcatgc accgcaacca gacttttaac cgcagttgcg cccaccatcg caaagtgatt
    35521 tatcagccca cttgccagca acaatacaaa gagtaacgca ttcgaccagt gccataatct
    35581 gaccgctttg ctgtaaagat aaaccttctc accatgatcg gtggctgatt tcttcacgcc
    35641 acgagcacga agcaaggcgt gcagccccaa caccaaccac atcccaacca gcaatactcc
    35701 ggcaatgatc aaccacaccg gccaaaattc gggagtgaac tgtggcacat agttcgctaa
    35761 ctggctctga aactgttcag catgttgcga cgggttcata cattttcctt tttaatcaaa
    35821 tgttgaccga accggcgata cagatgcggt ttcccggcac caagtagctg atattgatag
    35881 tatttatttt cctgcaacca cgcctgaatt tctgggctat cttcgcgacc aaagatcaat
    35941 gcgtgttccg gacaggcgct gacgcaaatg ggcggaaagc cttttgccag tcgggactca
    36001 gcgcagaaat cacatttatc tgccacttta gtgacagggt taaggtagcg aacctggtac
    36061 ggacaagcgc caatgcaata actgcagccg atgcactgcg atttttctac ccgcacgatc
    36121 ccctgctcat cacgccacga cgcccctgtc gggcatacgt cgatgcacgg agcatcttcg
    36181 cagtgctgac atgactggcg aaaaaagtga tactgcgttt cgttgtcgtt atcggtgact
    36241 ggaatgtgtg ctatcgataa acggcttccc tgagcgggga cgtggttagt tttacggcat
    36301 gcgcgggcgc aaatattaca gccattgcac cgtgactcgt catgaatcat ggcataccga
    36361 acttcctttt cttgcctcgt gttcgctaac agagaagacg cagaaccagt aaaaaagatt
    36421 actgttccca tccccagaac aaatttgcgt cgagtgaagg acatctgatt atcccctaat
    36481 gcagtggctg caactgtcca ccctatccat gacatctccc tgaaatttca ggggttctga
    36541 aactgctcta cctcgaacca ttcgcataag ccataagcct tgccataggt cataaatcgc
    36601 tcgctaaaca agtacaacac cgccgtcgat gactccacct gccgtatatc ggcatattca
    36661 ggtgcaacat ccatcgcggc gatcgcggct tcaatctgcg cttcctggaa gtaatatggc
    36721 gcttgcatca gcatggcgac tttgtaagga cgaggatacg tctggcactc aaaacgaacg
    36781 gcgtgtgcaa tggcgcgaca aatgtcctgc tccacaactt gcaaagacat cgccgcgtag
    36841 ttttcactca tggcctgcgt tgagtaatag taatcatcct gacttcccgt catacaagcg
    36901 atatccgcgt agtctccgtt ctgcttaatg ctttccagca aaactgacag ttcctcttct
    36961 gcaaccgaat agggtggcga gagaaacacg gtgcgcgcca ccagctgccc agccgcagaa
    37021 tgctggcgaa tgaactcagc caggatttca gcaggttccg gttcggttac cgtatcaacg
    37081 gggtgacgca tcccctgcaa ccattgccgc ttcgcctcgt caacttcgct gactttcgct
    37141 aacggcaatt catcctgatg gttcatctta cccattcctt acgcaggcag tagattgtgt
    37201 gctgccagat cggcggcaat atcttccagc cccagccgct gcaatgtttc gcgggttgga
    37261 caaccaagct gtgggtccca gcccatttct ttgtagaaca tcgtgagcga agcatgcata
    37321 tcgtcacgat ccattttgtc agtaccttca gtaaagaccg ggatctgcgg atccttgtcg
    37381 aaaacccagg aacagataag atcgtgttca ttacgcatat ctttggtttg catcagtttt
//
LOCUS       DAJVQG010000022        36656 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJVQG010000022 DAJVQG010000000
VERSION     DAJVQG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36656
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.022"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(141..1052)
                     /gene="gsiD"
                     /locus_tag="OQM83_001025"
     CDS             complement(141..1052)
                     /gene="gsiD"
                     /locus_tag="OQM83_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCS1519342.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1055..1975)
                     /gene="gsiC"
                     /locus_tag="OQM83_001026"
     CDS             complement(1055..1975)
                     /gene="gsiC"
                     /locus_tag="OQM83_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCS1519343.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(1993..3531)
                     /gene="gsiB"
                     /locus_tag="OQM83_001027"
     CDS             complement(1993..3531)
                     /gene="gsiB"
                     /locus_tag="OQM83_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCS1519344.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3551..5422)
                     /gene="gsiA"
                     /locus_tag="OQM83_001028"
     CDS             complement(3551..5422)
                     /gene="gsiA"
                     /locus_tag="OQM83_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCS1519345.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5409..6374)
                     /gene="iaaA"
                     /locus_tag="OQM83_001029"
     CDS             complement(5409..6374)
                     /gene="iaaA"
                     /locus_tag="OQM83_001029"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCS1519346.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6578..7813
                     /gene="moeA"
                     /locus_tag="OQM83_001030"
     CDS             6578..7813
                     /gene="moeA"
                     /locus_tag="OQM83_001030"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCS1519347.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            7813..8562
                     /gene="moeB"
                     /locus_tag="OQM83_001031"
     CDS             7813..8562
                     /gene="moeB"
                     /locus_tag="OQM83_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCS1519348.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8741..9403)
                     /gene="fsa"
                     /locus_tag="OQM83_001032"
     CDS             complement(8741..9403)
                     /gene="fsa"
                     /locus_tag="OQM83_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCS1519349.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            9534..10433
                     /locus_tag="OQM83_001033"
     CDS             9534..10433
                     /locus_tag="OQM83_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCS1519350.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10439..12871
                     /locus_tag="OQM83_001034"
     CDS             10439..12871
                     /locus_tag="OQM83_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCS1519351.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13017..13832
                     /gene="ybiV"
                     /locus_tag="OQM83_001035"
     CDS             13017..13832
                     /gene="ybiV"
                     /locus_tag="OQM83_001035"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCS1519352.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            13984..15249
                     /locus_tag="OQM83_001036"
     CDS             13984..15249
                     /locus_tag="OQM83_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCS1519353.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15216..16376)
                     /locus_tag="OQM83_001037"
     CDS             complement(15216..16376)
                     /locus_tag="OQM83_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1519354.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            16614..17915
                     /locus_tag="OQM83_001038"
     CDS             16614..17915
                     /locus_tag="OQM83_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1519355.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWGHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            17930..20293
                     /locus_tag="OQM83_001039"
     CDS             17930..20293
                     /locus_tag="OQM83_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HCS1519356.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLEFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(20473..22068)
                     /locus_tag="OQM83_001040"
     CDS             complement(20473..22068)
                     /locus_tag="OQM83_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCS1519357.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPEILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22287..23207
                     /gene="ldtB"
                     /locus_tag="OQM83_001041"
     CDS             22287..23207
                     /gene="ldtB"
                     /locus_tag="OQM83_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1519358.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23266..24384)
                     /locus_tag="OQM83_001042"
     CDS             complement(23266..24384)
                     /locus_tag="OQM83_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCS1519359.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24381..24848)
                     /gene="mntR"
                     /locus_tag="OQM83_001043"
     CDS             complement(24381..24848)
                     /gene="mntR"
                     /locus_tag="OQM83_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCS1519360.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25034..25162
                     /gene="mntS"
                     /locus_tag="OQM83_001044"
     CDS             25034..25162
                     /gene="mntS"
                     /locus_tag="OQM83_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCS1519361.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            25434..27017
                     /gene="opgE"
                     /locus_tag="OQM83_001045"
     CDS             25434..27017
                     /gene="opgE"
                     /locus_tag="OQM83_001045"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCS1519362.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27066..27581)
                     /gene="ompX"
                     /locus_tag="OQM83_001046"
     CDS             complement(27066..27581)
                     /gene="ompX"
                     /locus_tag="OQM83_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCS1519363.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(27634..27699)
                     /locus_tag="OQM83_001047"
     CDS             complement(27634..27699)
                     /locus_tag="OQM83_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1519364.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            27934..28821
                     /gene="rhtA"
                     /locus_tag="OQM83_001048"
     CDS             27934..28821
                     /gene="rhtA"
                     /locus_tag="OQM83_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCS1519365.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            29120..29623
                     /gene="dps"
                     /locus_tag="OQM83_001049"
                     /gene_synonym="pexB"
     CDS             29120..29623
                     /gene="dps"
                     /locus_tag="OQM83_001049"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCS1519366.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            30027..30773
                     /gene="glnH"
                     /locus_tag="OQM83_001050"
     CDS             30027..30773
                     /gene="glnH"
                     /locus_tag="OQM83_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCS1519367.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            30912..31571
                     /gene="glnP"
                     /locus_tag="OQM83_001051"
     CDS             30912..31571
                     /gene="glnP"
                     /locus_tag="OQM83_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCS1519368.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            31568..32290
                     /gene="glnQ"
                     /locus_tag="OQM83_001052"
     CDS             31568..32290
                     /gene="glnQ"
                     /locus_tag="OQM83_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCS1519369.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            32407..34632
                     /gene="ybiO"
                     /locus_tag="OQM83_001053"
     CDS             32407..34632
                     /gene="ybiO"
                     /locus_tag="OQM83_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HCS1519370.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(34629..35555)
                     /gene="rlmF"
                     /locus_tag="OQM83_001054"
     CDS             complement(34629..35555)
                     /gene="rlmF"
                     /locus_tag="OQM83_001054"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCS1519371.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            35831..36091
                     /locus_tag="OQM83_001055"
     CDS             35831..36091
                     /locus_tag="OQM83_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1519372.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            36356..>36656
                     /locus_tag="OQM83_001056"
     CDS             36356..>36656
                     /locus_tag="OQM83_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCS1519373.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         8843 a         9930 c         9233 g         8650 t
ORIGIN      
        1 gccgctgcaa aaatagtccc ttgctgcggc tttttaacca aattacccga ttctacaaaa
       61 tcagcagaaa gttacaacac ttgcttaggg caataatttc ctgtgaggtg attacccttt
      121 caagcaatat tcaaacgtaa ctatccttta attttcggat ccagcgcatc gcgtaaacca
      181 tcgcccaaca aattgaacgc cagtacggtc agaaaaatag ccagggccgg aaaaacagcg
      241 acatgcggcg cgataaccat atccgctcgt gcctcattga gcattgctcc ccactctggt
      301 gtcggcggct gcgcaccgag gccgagaaat gacaggctgg cggcagagat aatcgaggtg
      361 ccaatgcgca tggtgaaaaa caccacgata gacgagacgg tcccaggcaa gatatgacgc
      421 aataaaatgg tcatatcgct ggcaccaata ctgcgtgctg actcaataaa ggtttgctgt
      481 ttcaacacca gcgtgttgcc gcgcaccagt cgggcaaacg cggggatgga aaaaatggcg
      541 actgcgataa tcacgttagc aatgccgctt cccaacaccg caacaacagc gatcgccagt
      601 aaaatacccg ggaaggcaaa cagcacatcg caaatgcgca tgatcagccg atcccaccag
      661 ccttcataat atccagccaa caagcccagc aacgtcccga tcgccgcacc gataaacacg
      721 gcaaacacgc ccgccgccag cgagatttgc gcaccaacca ggacacggct gaaaatgtcg
      781 cgccccagtg aatcgacgcc aaaccagtgc tgcaaagaag gtccgttatt cagattgtca
      841 taatcaaaat aattttcggc gtcataggga gcgatccagc gtgcaaaaat ggccaccaca
      901 atcaataaaa taacgaataa tgcggcggtc atcgccatat gctgacggcg aaatcgtcgc
      961 cagaattcat gccacggtgt acgcacctgg tcaggtttga ccagtggcat ggcgtttaac
     1021 accgcctgac gtcgccagtt aaatagtcgc atccttactt gtacctgata gccgggttaa
     1081 tggcggcgta aagcacatcc accactaagt tgataagaat aaattccagc gagaaaagca
     1141 gaatttccgc ctgaatcacc gggtaatcgc gcatttctac ggagtcaacg agtaagcgtc
     1201 caagtccggg ccagttgaaa actttctcca ccacgataga accaccgagc aaaaagccaa
     1261 actgtaagcc catcatggtc actaccggga tcatcgcgtt acgtagcccg tgtttgagga
     1321 caacccaggt ttcgctcacc cctttcgccc tcgcggtacg catataatct tcgcttaaaa
     1381 catcgacaaa cgacgcgcgg gtaaagcgcg ccatcacggc tgccactgcc gcgccgagcg
     1441 tcagggaggg taaaatgtag tgctgccaac tgtctgctcc cacggtaggc agccagccca
     1501 gttcaacgga gaatacctga attaaaagca tccccagcgc aaatgccgga aacgagatcc
     1561 ccgacaccgc aatggtcata ctcaatcgat ccggccaacg attacgccag acggcggcga
     1621 taattcccgc cgccatacca aatatcaccg cccagaccat actggttatg gtcagccaca
     1681 gcgttggcat aaagcggctg gcaatctcat cggcaaccgg acgacgcgac accatcgaca
     1741 ggccaaaatc cccctgcaca gcattgctga tatagtgcca gaactggtga tacagcggct
     1801 gatccaaccc cagctgctga cgcaccagtt ctataacctg cgcatcagct tcgagcccgg
     1861 caatcaatcg cgccggatcg ccgggcagca tatgcacaaa taaaaacacc agcaccgaga
     1921 cgataaacag cgtcggaatc aaccccagta agcgtttgat aacgtaatta agcattccac
     1981 tccctgcgtt gcttattgca aatccgcgtc ttcaaagctg aagccggtgt ctggcatgat
     2041 ccaaaaaccg gtcaggtttt tactgtgtgc cgacaccagt ttttctacca ccagcgggat
     2101 ccacggcgac tcttgccaga tgatatcctg cgccgcctta tacaagcggg tcttttccgc
     2161 cggatcatta gttttcagtg cctgagccag gaagtcgtcc acctgtttat tgctgtaaaa
     2221 tgcggtatta aacagcgtcg gtggccagtt ttgcgaggcg aacagtggcg atagcgccca
     2281 gtccgcttcg ccggttgaag ccgaccagcc agtgtagaac atccgcacgc cgctctcttt
     2341 ttgcccttta ccttcaactt ctgccgcccg ctgtccggca tccatcgcgg tcacctgggc
     2401 tttaatcccg acctgcgcta actgctgctg ggtaaattgc agcactttct gcgcggtgct
     2461 gtggttatgt gacgaccaca gcgtggtact gaaaccgttg ggatatcccg cctctttcag
     2521 taattcgcgc gctttcactg gatcgtaagg ccacggttta taactttgcg cgtaggcgat
     2581 acttggcggt accacaccag tagctggcgt tgcatagccc gcaaaggcaa ctttcaccag
     2641 cgccggacgg ttaatggcgt aattcagcgc ctcacggacc ttcgggttat cgaacggctt
     2701 ttgcgtcacg ttcatactga tataacgctg cataattgac ggactggcca tcaactcgat
     2761 atttttgttt ttctccagca gtgtggcctg ctcgtaggga atggggaaag caaactgcgc
     2821 ttcaccggtt tgcagcattg ccgcgcgggt gttgttatcc gccaccggac gccaggttat
     2881 gctgtccagt ttgggcaatc ctggctgcca gtaacccgcg aattttttca ccttcacaaa
     2941 atcggtctga ttccaggtat ccagttcata cggtccggtt cccaccggat gaaaaccaat
     3001 ctccttgcca tatttttcca gcgctgccgg tgaaatcatc gcggtcgccg gatgggcaag
     3061 aatattaata aacgctgaga acggctgttt gagggtaatc tttaccgttg tcggatcgat
     3121 cgcctccgtt ttggcaatat tcttatacag gttatagcgt ttaagatgat tcgccggatc
     3181 gctggcccgg tccagattcg cttttaccgc cactgcgttg aaatcggtgc catcctggaa
     3241 tttaatgcct tcccgcaatt tcacggtgta agtaatgccg tcatcggaaa cggtataact
     3301 ctccgccagc acgtttttca gtttcatctc tttatccaga ccgaacagcc cctggtaaaa
     3361 cgatttcgct acggcctgag ataacgtatc attcgcgtca tacggatcga gcgtggtgaa
     3421 attcgatcct accgccacca ccacatcttt ggcagcgaat gcacaagatg ccatcaacgc
     3481 tgtcgcaatg cccagcgcca ctaacccact acggtgtaca gctcttgcca ttttattctc
     3541 cgcctgaatg ttatctacgc atgaatgcgt attctgattg tggttgtgcg acgtaatgcc
     3601 ccggcccgac gcattgcaac gagacggctg ccacctcttc gccacgcaga tgaatattgc
     3661 tgggaagatc gtccgacagc agtacacgct gcggtcgttg tcgggacggt tcagcgaccg
     3721 gaactgccgc cagtaattta cgcgtataag gatgctgcgg gttttcgaag accgcgcgcc
     3781 gtggaccaat ttcaacaatt tgcccgagat acatcaccgc cacacgatga ctaatccgct
     3841 ctaccacggc catatcgtgg gagataaaca gatacgcaat gccgaaatca cgctggagat
     3901 cgagcaacaa gttgataatc tgcccgcgaa tagaaacatc cagcgccgaa acggcttcgt
     3961 cggcaatgat cacttttgga ttcaatgcca acgcgcgagc aatgcagatg cgctggcgct
     4021 gaccgccgga aaactcatgc gggtaacgcc aggcatgttc aggtaacagg cccacgcgct
     4081 ccagcaacca cgcaacgcgt gcagccgcgt ctttacctgg caataaaccg tgtacacgca
     4141 gcggttcgat aatcgaatca ccgatggtct gacgtgggtc cagcgaagcg taagggtcct
     4201 gaaaaataaa ctgaatatcc cggcgcaatg cctgaagttt gccgggtgac aaggtatcga
     4261 ttctctgacc gttaaagata atttcgccgc cctgcgattc gaccaggcgc agcaacgccc
     4321 gcccggtagt ggatttaccg ctgccagact cgcccaccag cgatagcgtt tcgccaggcc
     4381 agagatcaaa actgactttc tcaacggcat gcacttcccg cgttacgcga ttcaacaaac
     4441 cgctgcgcaa agggaaacgg gtgacaagat tacgcactcg taaaacaggt tcgccatcca
     4501 ccaccgtttt ctgctcgatg gggggggcct gtttcgctgg atgttcaagc gatatcaacg
     4561 ggaaacgtcg gggataatcc aaccctttca tcgcaccaag ttgcggaaca gcagctaaca
     4621 gcgcacgggt gtaaggatgt tgcggtgcat gaaaaatctg ttcgacggta cccgtttcca
     4681 ccgcctcgcc ctgatacatc accagtaccc gatcggcaat ctctgccacc acgcccatat
     4741 cgtgagtgat aaagataacg cccatcgaca tctctttttg caatactttg attaattgca
     4801 ggatctgcgc ctgaatagtg acatccagcg cggtggttgg ctcatcggca atcagcaccg
     4861 ccgggcggca tgacagcgcc atcgcaatca tcactcgctg gcgcatcccg ccagagagtt
     4921 gatgcggata acgtgaaaga atggtttgtg cctcaggaat gcgtacctga tccagcatcc
     4981 gcttcgcctc gaccatcgct tcttcacgac tggcgttctg atgcagacga attgattcgg
     5041 caatctgttc acccacagta aataccgggt tcagcgatgt catcggctcc tgaaatatca
     5101 tcgccatatc cgcaccgcga acatggcgca tttgtgcagc gctctgctcg ctaagttcaa
     5161 tcacttcgcg actgcgccgc tgcaacagca ttttatcgca ctgtactaaa ccgcccgcct
     5221 gttccaacag gcgcatcaat gccaacgcag tcactgactt accggagccg gattcgccaa
     5281 caattgccag cgtctcaccg cgttgcagac taaaagagag attgcggacc gcagctattt
     5341 tctgctggtc ctgcataaag gcaatattca gattttcaac cgccagcaca ttaccggcat
     5401 caagttcatc actgtgtggc aacggtgtcc cctttttcac ggtagatacc ggtggttggc
     5461 gtatcgcctg cgtagcccca ggcgcgatac attccttcgg tgttaaacgg tagcgcgaca
     5521 ttcccttcat ggtcgatagc gattaagcca ccgctaccgc caagcgcagg gagtttttcc
     5581 attactaccc gctcgcaggc ttccgcgaga cttaatccgc cgtaatccat taacgcggcg
     5641 atgtcatatg ccgccagcgc gcggatgaag acttcgcccg tgccgataca agaaaccgcc
     5701 acactggcgt tattggcgta gcatccggca cccactaagg gactatcgcc aactcgtccg
     5761 ggtaatttat tggtcattcc gcctgtggac gtggctgccg ccaaattgcc gtctaaatcc
     5821 aacgccacgg cccccacggt gcccattttc tgtttttcat ccagtggcgc accgctatgg
     5881 tcgaggacgg ttgccccttc cttgcgcgct gccagtagtt gttcataacg caaaggcgtg
     5941 gagaaaatct ccggcgagac gcgctccatg ccacgagcaa acgcaaaatt ttctgcccct
     6001 tcgccaatca tcatcacatg cgggctttgc tccatcacca gccgggcggc aagaaccgga
     6061 ttacgcagat gactaacgcc cgccaccgca ccggctttca gggtgttacc atccatcaca
     6121 caggcgtcca gttcatgggt ttcatcacgc gtaaagacag cgccaattcc ggcgttaaac
     6181 agtggacact cttccagcag acgcaccgct tccgtcacca catccagcgc actttcgccc
     6241 gcttccagca ttttctgccc ggtttcaaca atggcagaca acgcctcaat gtagcgtaat
     6301 tcctgttgca gactcatctg cgcgcggcta attgcacctg cgccaccatg aattgcaatg
     6361 actgctttgc ccatagtatt cgtccattaa gggtggctcg ccacttttgc tataaatatc
     6421 agaattttta tgaattactc attcaatttt tgaatataga aagatggata tggcatgtaa
     6481 aggcaggagc caacgctacg cccgaggtgg catacagtct gtggcgtttt tctgacataa
     6541 taggcaaatt cgattttgcc tccgcaggag tgttttcatg gaatttacca ccggattgat
     6601 gtcgctcgac accgcgctta atgagatgct ttctcgcgtc accccactga ccgcccagga
     6661 aacgctgcca ctggtacagt gttttggtcg tattctggcg agcgatgtcg tttcgccgct
     6721 tgatgttccg gggtttgata actccgcaat ggacggctac gcggtgcgtt tagccgatat
     6781 tgcctccggg caaccgctgc ccgttgccgg taaatccttt gccggtcagc cgtatcatgg
     6841 tgaatggcct gcgggcacct gcattcgtat tatgaccggt gcgccggtgc cggaaggctg
     6901 cgaagcggtg gtgatgcagg agcagactga acaaacggac aatggcgtgc gttttactgc
     6961 tgaagtgcgt agcgggcaaa atattcgccg tcgcggtgaa gatatctctg caggtgcggt
     7021 tgttttcccg gcgggaactc gcctgactac cgcagagctg ccagtaattg cttcactggg
     7081 gattgccgaa gttccggtga ttcgtaaagt gcgtgtagcg cttttttcta ccggtgatga
     7141 actccagttg cccggtcagc cgctgggcga cggccaaatc tacgacacca accgtctcgc
     7201 cgtacacctg atgttagaac agctgggttg cgaggtaatc aacttaggga ttatccgcga
     7261 cgatccccat gccctgcgcg ccgcatttat tgaagccgac agccaggcgg atgtagtgat
     7321 cagttccggc ggtgtttcag tgggtgaagc ggattacacc aaaacgattc ttgaagagct
     7381 gggggagatc gccttctgga agctggcgat taaaccaggt aaaccgttcg cgttcggtaa
     7441 actcagcaat agctggttct gcggcctgcc gggcaacccg gtttcagcga cgctgacctt
     7501 ctatcaactg gtacagcctt tgctggcaaa actaagcggc aacaccgcca gcggcctgcc
     7561 cgcgcgccag cgcgtacgca cagcgtcccg cctgaaaaaa acgccaggac gtcttgattt
     7621 ccagcgcggc gtgctgcaac gcaacgccga cggtgaactt gaagtgacga ccaccggaca
     7681 tcagggttca catatattta gctcctttag tctcggcaac tgctttatcg tgctggaacg
     7741 cgatcgcggc aatgtggacg tgggcgaatg ggtggaagta gaaccgttta acgcgttgtt
     7801 cggaggcctg taatggcgga actcagcgat caggagatgc tgcgctacaa ccggcaaatc
     7861 attctgcgcg gttttgattt tgacggtcag gaggcgctaa aagattctcg tgtgctgatc
     7921 gtcggcctcg gtggactcgg ctgcgcagct tcgctgtatc tggcaagcgc cggtgtcggc
     7981 aacctgacgc tgctcgactt cgacacggtt tcgctctcga atctgcaacg ccagacactg
     8041 cacagtgatg ccacggtcgg gcaaccgaag gtggaatccg cccgtgacgc cctgacgcgg
     8101 atcaacccac atatcgcgat tacgccagtc aatgcactgc tagatgacgc agaacttgca
     8161 gcattgattg ctgaacacga tctggtgctc gactgtacgg ataacgttgc ggtacgtaat
     8221 caactgaacg caggctgttt tgccgcgaag gtgccgctgg tttccggcgc ggcaattcgt
     8281 atggaagggc aaatcacggt cttcacttat caggacggcg aaccgtgcta tcgctgcctt
     8341 agccgtttgt ttggcgagaa cgcattaacc tgcgtagaag caggcgtgat ggcaccgttg
     8401 atcggcgtaa ttggttcgtt gcaggcaatg gaagcgatca aactgctggc aggttatggc
     8461 aaaccgacca gcgggaaaat cgtcatgtac gatgcgatga cctgtcagtt tcgcgaaatg
     8521 aaactgatgc gcaatccggg gtgtgaggtg tgcgggcagt aattgccacc tccaggcctg
     8581 ataagacgcg acaagcgtcg catcaggcat ttgctccaga cgccagatgc ggcgtgaacg
     8641 ccttatctgg cctacggttc gggcacagat ttccaagcgc ggcaggcatc ggtgcactcc
     8701 gccacgtcat cagcaattta tgaggatgca gaacgtccgg ttaaatcgac gttctgccaa
     8761 acgctccctg ccagtcctgc tcaaacttcg ccacagcggc ttccaccgcc ggatagctaa
     8821 tcatctgttg tgccacatcc agtggcagag taattgattc acatcctgcc agtaagcagt
     8881 ccagcgcctg acgcggggtt ttgaaactcg ctgccagcac tttcgcctgc ggcgcatgca
     8941 ttttcaataa ctggtgtaag tcggtcacag tctgaatgcc gctaccgccc tgagcatcaa
     9001 tacgattaac gtaaggcgca acatattccg cacctgccag cgccgacagc aacccttgtg
     9061 ctgcgccata taccgcggtt cccagcgttg gaatcccttc cgcttttaac atcttaatag
     9121 ctgccagccc ctcggcggtc accggaactt tcaccacgat atccgcaata atagaacgca
     9181 atttacgtgc gtcattcacc atcccttcag cagtggtagc cattacctgg gcaaacagac
     9241 gcccctgacc gcccatcgct tcatgaagtt gcggaagcac aacatccagc ggttttttac
     9301 ccgcggcgat aatgcttggg ttagtggtca cacccgccag cggaaaaata cgtgacagcg
     9361 ccttcaccgc aacaacgtct gaagtatcca gatacagttc cataaccatc ctcaaaatgt
     9421 actttttgtc tgaatgtaga ctatcacggg aaaactctct gaacagttga cgcacatcaa
     9481 tctaactttc attcgaaagt attttaatgt ttgaacgaaa cagagaggcg actatgattt
     9541 tcaatattca gcgctactcg acccatgatg gccccggtat ccgcacggtc gtatttctta
     9601 aaggctgttc gctgggctgc cgctggtgtc agaacccgga aagccgcgcc cgcacgcagg
     9661 atctgctgta tgacgcacga ctgtgtctgg aaggctgcga gctgtgcgct aaggccgcgc
     9721 cggaagtgat tgagcgcgcg ctgaatggtt tgcttattca tcgggaaaag ttaaccccgg
     9781 agcatctgac ggcgttaacc gactgctgtc cgacacaggc attaaccgtg tgtggtgaag
     9841 tgaaaagcgt tgaggagatc atgacgaccg ttctgcgcga taaaccgttt tacgatcgca
     9901 gcggcggcgg tttaacgctt tcgggtggtg agccctttat gcagccggaa atggcgatgg
     9961 cgctactgca agccagccac gaggcaggca ttcatactgc ggtagaaacc tgtctgcatg
    10021 tgccgtggaa atatatcgcc ccttctctgc cctatatcga tctgtttctt gccgatttaa
    10081 aacacgttgc cgacgcgccg tttaaacagt ggaccgacgg taacgccgcc agagtgctgg
    10141 ataacctgaa aaaactcgcc gcagcgggca aaaaaatcat tatccgcgtg ccgctgattc
    10201 agggctttaa tgccgacgaa acctctgtaa aagctattac cgattttgcc gccgacgagc
    10261 tgcacgttgg cgaaattcat ttcctgccct accacacgct aggcatcaac aaatatcact
    10321 tacttaatct gccctatgac gccccggaaa aaccgcttga tgcgccagaa ctgctcgact
    10381 ttgcccagca gtatgcctgc cagaaagggt taaccgcgac cttacgagga taacaaccat
    10441 gaccacactg aaactggaca cgctcagcga ccgcattaaa gcgcacaaaa atgcgctggt
    10501 gcatattgtg aaaccgccag tctgtaccga gcgcgcgcag cactataccg agatgtatca
    10561 acaacatctc gataagccga tcccggtacg tcgcgcgctg gcactggcgc atcacctggc
    10621 gaatcgcacc atctggatca aacacgatga gttgatcatt ggcaaccagg caagcgaagt
    10681 tcgcgccgcg ccgatcttcc cggaatatac tgtctcgtgg atcgaaaaag agattgatga
    10741 tctggcagat cgtcccggtg ctggctttgc ggtgagcgaa gagaacaaac gcgttctgca
    10801 tgaagtgtgc ccgtggtggc gcggtcagac cgtacaggat cgctgctacg gcatgtttac
    10861 cgatgagcaa aaaggtctgc tggcgaccgg aatcattaaa gcggaaggca atatgacctc
    10921 cggcgatgcg cacctggcgg tgaatttccc gctgctgctg gaaaaaggtc ttgatggtct
    10981 gcgcgagaaa gtagcggaac gtcgctcgcg catcaatctg acggtactgg aagatttgca
    11041 cggcgaacag ttcctgaaag cgattgatat cgtgctggtg gcagtcagcg aacacattga
    11101 acgttttgca gccctggcgc gtgaaatggc cgcgaccgaa acccgcgaaa gccgtcgcga
    11161 tgaactgctg gcgatagcag aaaactgcga tcttatcgcc caccagccgc cgcagacttt
    11221 ctggcaggcg ctgcaactgt gttacttcat ccaattgata ttgcagatcg aatctaacgg
    11281 tcactcggta tcgtttggtc gtatggacca gtatctctac ccgtactatc gccgcgacgt
    11341 tgaactcaac cagacactgg atcgcgaaca cgccatcgaa ttgctgcata gctgctggct
    11401 gaaactgctg gaagtgaaca agatccgctc cggctcacac tcaaaagcct ctgcgggaag
    11461 tcctctgtat cagaacgtca ccattggcgg acaaactctg gttgatggtc aaccaatgga
    11521 cgcggtgaat ccactctctt acgctatcct cgaatcctgc ggtcgcctgc gttccactca
    11581 gcctaacctc agcgtgcgtt accatgcagg aatgagtaat gatttcctcg acgcctgcgt
    11641 acaggtgatc cgttgcggct tcgggatgcc ggcgttcaac aacgacgaaa tcgtgatccc
    11701 ggaatttatt aaactcggta ttgaaccgca ggacgcttac gactacgcag cgattggttg
    11761 tatcgaaacc gccgtcggtg gcaaatgggg ctatcgctgt accggcatga gctttatcaa
    11821 cttcgcccgc gtgatgctgg cggcgctgga aggcgggcgt gatgccacca gcggcaaagt
    11881 gttcctgcca caagaaaaag cgttgtcggc aggcaacttt aacaatttcg atgaagtaat
    11941 ggacgcgtgg gatacgcaaa tccgttatta cacgcgtaaa tcaatcgaaa tcgaatatgt
    12001 cgtcgacacc atgctggaag agaacgtgca cgatattctc tgctcggcgc tggtggatga
    12061 ctgtattgag cgagcgaaaa gtatcaagca aggcggcgcg aaatatgact gggtttctgg
    12121 cctgcaggtt ggcattgcca acctcggcaa cagcctggcg gcagtgaaga aactggtatt
    12181 tgaacagggc gcgattggtc agcaacagct ggctgccgca ctggcggatg acttcgatgg
    12241 cctgactcat gagcaactgc gtcagcggct gattaacggt gcgccgaagt acggcaacga
    12301 cgatgatacc gtcgatacgc tgctggctcg cgcttatcag acctatatcg acgaactgaa
    12361 acagtaccat aacccgcgct acggtcgtgg tccggttggc ggcaactatt acgcgggtac
    12421 ctcgtctatc tccgctaacg taccgtttgg cgcgcaaact atggcaacac cggacgggcg
    12481 taaagcccac accccgctgg cagaaggcgc aagcccggcc tccggtactg accatcttgg
    12541 ccctactgcg gtcattggct cagtgggtaa actgcctacg gcagcgattc tcggcggcgt
    12601 gttgctcaac cagaaactga atccggcaac gctggagaac gaatctgaca agcagaaact
    12661 gatgatcctg ctgcgtacct tctttgaagt gcataaaggc tggcatattc agtacaacat
    12721 cgtttcccgc gaaacgctgc tggaggcgaa aaaacatccg gatcagtacc gcgatctggt
    12781 agtgcgtgtc gcgggctatt ccgccttctt caccgcgctc tctccagacg cacaggacga
    12841 tatcatcgcc cgtactgaac atatgctgta atctcctcgg cccggcggcg tcgtcgccgg
    12901 gctaaatcat tcacatcatc aatttcatcc ttactttcat tcgaaatata atttgtgctc
    12961 tgcgtcacat tgttattaca ctgttacggt ttcattttcg agccaggagc acacctatga
    13021 gcgtaaaagt tatcgtcaca gacatggacg gtacttttct taacgacgcc aaaacgtaca
    13081 accaaccacg ttttatggcg caatatcagg aactgaaaaa gcgcggcatt aagttcgttg
    13141 tcgccagcgg taatcagtat taccagctta tttcgttctt tcctgagcta aaggatgaga
    13201 tctcttttgt cgcggaaaac ggcgcactgg tttacgaaca tggcaagcaa ctgttccacg
    13261 gcgaactgac ccgacatgaa tcgcggattg ttattggcga gttgctaaaa gataagcaac
    13321 tcaattttgt cgcctgcggt ctgcaaagtg catatgtcag cgaaaatgcc cccgaagcat
    13381 ttgtcgcact gatggcaaaa cactaccatc gcctgaaagc tgtaaaagat tatcaggaga
    13441 ttgacgacgt actgttcaag ttttcgctca acctgccaga tgaacaaatc ccgttagtga
    13501 tcgacaaact gcacatagcg ctcgatggca ttatgaaacc cgtcaccagt ggttttggct
    13561 ttatcgacct gattattccc ggtctacata aagcaaacgg tatttcgcgg ttactgaaac
    13621 gctgggatct gtcaccgcaa aatgtggtag cgattggcga cagcggtaac gatgcggaga
    13681 tgctgaaaat ggcgcgttat tcctttgcga tgggcaatgc tgcggaaaac attaaacaaa
    13741 tcgcccgtta cgctaccgat gataataatc atgaaggcgc gctgaatgtg attcaggcgg
    13801 tgctggataa cacatcccct tttaacagct gacctctcta ccggagccgt gcgctccggt
    13861 tttcccttct ttttacatcc tgtacatcac atcaaatcat taaacttgca ttaacttatt
    13921 tataacttat attgtcgcat gcaaatcaga atggtttcga atgaaagtta atgagaggtg
    13981 gttatggctt ctacttttac cagcgacaca ttgcctgccg atcacaaagc agctatccgt
    14041 cagatgaagc acgcgctgcg ggcgcagctt ggcgacgtcc agcagatctt taatcagcta
    14101 agcgatgaca ttgccacgcg agtggctgaa atcaacgcac tcaaagcaca gggcgatgcc
    14161 gtctggccgg tgctgtctta tgccgatatc aaagcaggtc atgttactgc agagcagcgc
    14221 gaacagatta aacgtcgcgg ttgtgcggtg ataaaaggcc atttcccccg cgaacaagcg
    14281 ctaggctggg atcagtcgat gctggactat ctggaccgca accgctttga cgaggtctac
    14341 aaaggccccg gcgataattt cttcgggacg ctcagcgctt cacgtcccga gatttacccc
    14401 atctactggt cgcaggcgca aatgcaggcc cgccagagtg aagaaatggc gaatgcgcag
    14461 tcgtttctca atcgtctgtg gacatttgaa agtgatggaa agcaatggtt taacccggat
    14521 gtgagcgtca tctaccctga ccgtatccgc cgccgtccgc ccggaacgac ctccaaaggt
    14581 cttggagcgc ataccgactc cggggcgctg gaacgctggc tgcttccagc gtatcagcgc
    14641 gttttcgcca acgtctttaa tggcaatctg gcgcaatatg atccctggca tgcggcacat
    14701 cgtacggaag ttgaagagta cacggtggac aacaccacca aatgttccgt gtttcggaca
    14761 ttccaaggct ggacagcgct ctctaatatg ctgcctggtc aggggctgct gcacgttgtg
    14821 cccattcctg aagccatggc gtacgtactg ttacgtccgc tgcttgatga tgtgccggag
    14881 gatgaactgt gcggcgtagc gcccggaaga gtgttgccgg tatcagagca atggcatcca
    14941 ctgttaattg aggcgttaac cagcattcca aaactcgagg ccggagactc cgtctggtgg
    15001 cactgcgacg tcatccattc cgttgccccc gttgaaaatc aacagggttg gggcaacgtg
    15061 atgtacattc ctgcggcacc aatgtgcgag aaaaatcttg cctacgcgca caaggtgaag
    15121 gccgcactgg aaaaaggcgc atcgccgggc gacttcccgc gcgaggacta tgaaacaaac
    15181 tgggaaggac gctttacgct tgccgacctc aacattcacg gtaagcgagc gttgggaatc
    15241 gacagctgag tgtcgtacag cccttcccgt tccacggcgg tgttcccttt tcctgggcga
    15301 acccgccgca ctgactcccg caccgccaat cgtccgtgca gcagcagcga gcccaccgga
    15361 gcatccgggc gaatcagccg ctgttgcaac atttgcaccg cttcattgcc tagctcatcg
    15421 cgtggcacat gcacagccgt caggggaaca tcctcgattg ccgcaaggtt aaagccgtcg
    15481 atactcatca ccgagacatc ctgtggtacg cgcagaccac gacgatgtag cgcatttacc
    15541 gttcccgccg ccataaaatc cccgccgacc agaaacgccg tcggcaacgt cttgccattg
    15601 gcggcatcca gccacgctcc gaccttttct tccgcttctt tagcgctaaa actgctgata
    15661 ttcagcaaat gacggtcatg gtgaaaggcc agattatgct gacgccatga ttctttaatc
    15721 cctgcaaacc gctgctccat agtgtagcgg cgcaggcaca tcacattcat cacctcccgg
    15781 tgacccattt caaacagata gcgggcggcg aacgcgccaa ttaaccgatg atccggcgca
    15841 atcgccggga ggcgcattcg ttcatcacgg cagttgatca gcacacaagg cttagcgaga
    15901 tcggcggcca gatcatggat gtgcggatcg tcaatcccca gcaggatcgc tgcctgcgta
    15961 tctgcttcat tcatccgcga gagaaagagc gcgggatcgc tgtcaaactc ctccagcgcg
    16021 cagtaacgca gccgcacttc atgcggcgat aacgctttgc tgatgctctg gatcacacgg
    16081 taataaaaga tatcagatcg ttggtcaaaa gcgcgttgcg gcgcgaaaat aatcaggttg
    16141 ttcagcaaca tacgacctgc tgccatgcca tccatcacgc ccaactcacg cgcgcaggcc
    16201 agcacctgtt cgcgagcgcg tgcactggta ttcgctttgc cagccagcac ccgcgaaacc
    16261 gtactcggcg atagcccggt acgtgcggcg atatcggcga ttttcagctt cttgtccatt
    16321 ttgtgatctg cctctaattc ggaaatgaca gaattttcat gagcaaaaaa gcccattctg
    16381 tctggcatta aatccatttc agtaatgtat atcagtcact catggaaaaa cgtgcacaaa
    16441 acccgctttc tttcgctttt agggtctctt actgtgaatt ggtagaacaa cttccataca
    16501 aaaataatgg aaaaacgaac aataaagact ttatttatca aaaagttaaa agtggtgtaa
    16561 caactccatt ctgacgttgc cgcacaaacc ctataccatt ctgtggagtg catatgagtc
    16621 agggtatcaa taacgatatg actgccagta aaccgcgcca tatcgtcaaa aatctgcgtt
    16681 ggtggatact ggtccttttt ttgctcggtg ttactgtcaa ctacatcacg cgtaactcgc
    16741 tgggtattct cgcccccgaa ctgaaaagca gcctagggat taccaccgaa caatattcct
    16801 ggattgtagg tgctttccag ctcgcctata ccctattcca accgttgtgc ggatggctga
    16861 ttgacgtcat tggcctcaaa ctgggcttta tgatctgcgc gggactgtgg gcactggcct
    16921 gtctgatgca tgcgggggcg ggaaactggg gacatctggc gctgttgcgc ttctttatgg
    16981 gcggtgcgga agccgcggca accccagcaa acgccaaaac catcggtgaa tggtttccaa
    17041 aatcagaacg cccgatcgcc gcaggctggg caggcgtcgg tttttccatc ggtgccatgc
    17101 tggccccacc gattatctat tttgcccacg cttcattcgg ctggcagggt gcatttatgt
    17161 tcactggcat cctggcgatg ctgtgggtgg tgctgtggtg ggcgttctat cacagcccgg
    17221 ataagcatcc taacctcagc aaagccgagc tggactttat tcgccaggat aacgaagcgc
    17281 ccccggtcaa gttacccttt ctgaccgcac tgaaaaccgt gggcaaaaat aaacgctttt
    17341 acggtatcgc tatcccggcc tttatggcag aaccggcgtg ggcggtgatg agtttctggg
    17401 tgcctctata cctgtccaaa acctatggca tggacctgaa gcagatcgca ttattcgcct
    17461 ggttgccgtt cctcgccgct gatcttggca gtattgccag cggctatctg accaaacttt
    17521 acacccgcct gtttgggctg agtcgtgtga actccgttgt cgcatcatcg gtcagtggcg
    17581 cattcttgat ggtttcgctg gcggtgatgg cctttaccaa taacccgtat atcgccatcg
    17641 tccttatctc catcggcggc ttcggtcacc agatcatctc ctgcatgctg agcgcactgg
    17701 tggtggaatc cttcgacaaa ggtcagatgg ccactgttaa cggcatgcgc ggttctgccg
    17761 cgtggatcgc aagtttcctg ttctccctga ttatcggggt taccgctgac aaaatcggct
    17821 ttaaccccct gtttatcgcc atgggcttct ttgacctgat tggcgctttc ttcctggtca
    17881 cctttattgc tgaacgtcgc gcaaaacgcg cctgagagtg gatgtaaaca tgaaaacgct
    17941 gaaaaactgg accctgcaca aacagtctgc ccaccacgtt gaactgctgg tcgatggcca
    18001 gcatatcctg tgcctgtacg tgctggaaga aaatctgttc cgcgtgctgt taaaacgcaa
    18061 aggcgagctg gcgctagatc gcacctggag cattgctccg gcgcaggatg tgccatggga
    18121 agggcgcagc cgcgaagatc tcagcggctt tagcctgccc gcgtggacag tgatccagca
    18181 cgacgacacc gtggttatcg ccaccgagtc actgcgtgca accgttcacc agccgctgtg
    18241 gctggagtgg cactatcgta acaacgaagg cgaatggctg ccgttggtga atgaccgccc
    18301 gaccagcgct tatctgctga atgcccacgg cgatggcgtg gcacactatc agagccgtcg
    18361 taaagatgaa cgcttttatg gcctcggaga taaagccggc gatctgcaac gcaacggtaa
    18421 acgttacgag atgcgtaacc tcgacgcgat gggctacaac gccgttagta ctgacccgct
    18481 gtacaaacac attccattca ccatcacccg ccgcgatgac attagcttcg gcctgtttta
    18541 tgacaacctc agcagctgct ggctggatct gggcaacgag atcgacaact accacaccgc
    18601 ctatcgccgc tggcaggcgg aagcgggtga tatcgactac tacctgttta ccggcgaacg
    18661 cgtacttgat atcaccaaag ccttcgtacg cctgaccgga aaaaccctgt tcggaccgaa
    18721 atggagcctg ggctacagcg gttcaactat gcactacacc gatgcgccgg acgcacaaaa
    18781 tcagttgatg aactttatcc gcctgtgtga agaacatgca attccctgcg attcgttcca
    18841 gctctcttcc ggttatacct caattaacgg caaacgctat gtgttcaact ggaactacga
    18901 caaagtgccg cagccgaaag tgatgagcca ggcatttcac aatgctggtt tgcacctggc
    18961 cgccaatatc aagccgtgcc tgttgcagga tcacccacgc tataacgaag tggcagaaag
    19021 aggactgttc atccgtgatt cagaaacaga tgaaccagaa cgttccagct tctgggatga
    19081 cgaaggttct aacctcgagt ttactaatcc gcagaccatc cagtggtggc aggaaggcgt
    19141 gaccacacag cttctggaga tgggtatcga ctccacctgg aacgacaaca acgaattcga
    19201 agtatgggac ggtgaagcac gctgtcacgg ctttggtcag acgatcgcga tcaaacatat
    19261 tcgcccagtg atgccactgc tgatgatgcg agcctcgatg gaagcccagc agcgtttcgc
    19321 tccggaaaaa cgtccgtact tgatctcccg ctccggctgc gcgggaatgc agcgctacgt
    19381 gcagacctgg agtggcgata accgcactag ctgggacact cttcgctaca acacccgcat
    19441 gggtctgggg atgagcctct ccgggctgta taacctcggt cacgacgtcg gcggtttctc
    19501 cggtgataag ccggacccgg aactgtttgt acgctgggta caaaacggcg tgatgcatcc
    19561 gcgctttacg attcactcat ggaacgatga ccataccgta aacgaaccgt ggatgtatcc
    19621 gggcgtcact ccggcgatcc gcagcgctat tgagttgcgc tatcgcctgc tgccctactt
    19681 ttataccctg atgtggcagg catacgccga tgatgaacca atgctacgtc cgactttcct
    19741 tgaccacgag cacgacgttc agacttttga agagtgtgat gacttcctgc ttggtcgcga
    19801 tattttggtt gccagcgtcg tagagcaagg cgagcgccag cgccgtgtat ggctgccaga
    19861 taacgaaact ggctggtacg atttctacaa cggcgaatgg ttcagtggcg gtcagtggat
    19921 caccctcgac gcaccgctgg agaaactgcc gctgatggtt cgtgccgggg ctggtctacc
    19981 gctcagcaaa cgcatcacct atgtgagcgc agaacaagac gatacccgcg aactgaaact
    20041 gttcccgctg aaaggtgtgg gcacaacgtc cggcctgctg tttgaagacg atggcgaaag
    20101 ctggggctac caaactggta atgcgctttg ggtggaatgg gaaatggttt gcgatggggc
    20161 gaccatcaac ctgaaagtca acgcgcgcgg ggattatcgt cctgcatgga gtgcactgaa
    20221 agtgtcatta ccattggaag aaaaacgcac gctgctggtc aatggtgttg aagggagtga
    20281 atggatgcgt taaatcatta ccggggccgg gaggcgactg caccttaccc ggtccagcca
    20341 tcgaatcctt tacctcagta tttcacgctg caggaaggca gcaaacctgt aaaccccggg
    20401 agcttaaata agtaagtgac cggggtgaac aggtgaagcc aatgcatctg ccgcatgaaa
    20461 gacggcgggg aattaagcgt cgatcccttt actacgcagg taatcttcgt aattaccgct
    20521 aaagtcgatc gcgcgttccg gggtgatttc cagaatgcgg gtcgccaggg agcttacgaa
    20581 ctcacggtcg tgtgaaacaa agatcagcgt gccctgatac agttccagcg ccatgttcag
    20641 tgactcgatg gattccatat ccaggtggtt ggtcggttcg tccatgatca gaatgttcgg
    20701 cttctgcatc attaacttac caaacagcat ccgccctttt tccccaccgg aaagcacttt
    20761 agctggcttt ttgatgtcgt cctggctgaa cagcaaacga ccgagaatgc tgcgtaccgc
    20821 ctgctcgtca tcgccttcct gcttccactg actcatccat tcgaacacgg tcagatcatt
    20881 ttcaaactca tattcgtggt cctgggcgta gtaaccaatg cgcgcgtttt cagaccattt
    20941 tacggtgccg ctgtccggtt gcagatcgcc caccagcgtt ttcagcagcg ttgatttacc
    21001 gacgccgttg gtacccagta ccgcaagttt ttcaccgacc tccagcagca ggttgagatt
    21061 tttaaacagt ggaccgttat caaacccttt ggtcagacct tccacttcca gcgcgttacg
    21121 gaacagtttc ttatcctgtt caaaacggat gaacgggttc tgacggctgg aggctttcac
    21181 ctcttccagt ttgattttat caatctggcg cgcgcgcgaa gttgcctggc gagatttcga
    21241 ggcgttggcg ctaaagcggc taacgaaaga ttgcaactca gcaatctgcg ctttcttctt
    21301 ggcgttatcg gccagcagac gttcacgcgc ctgggtcgcc gccgtcatgt actcatcgta
    21361 gttacccgga taaacgcgca gctcgccgta atccagatcc gccatgtggg tacagaccat
    21421 gttaaggaag tgacggtcgt gcgagatgat gatcatggtg ctgtcacgct cgttcagcac
    21481 ctgttccagc cagcgaatgg tgtcgatgtc gaggttgttg gtcggttcgt cgagcaggag
    21541 aatctccgga tcagcaaaca gcgcctgcgc cagaagcaca cgcagtttcc agccaggagc
    21601 aacttcactc atcgggccgt agtgctgttc cactggaatt cccacgccaa gcaacagttc
    21661 accggcgcga gcttccgcag agtaaccgtc catttcgccg tatttaactt ccagatcggc
    21721 cactttatag ccgtcttcct cactcatttc cggcaaagca tagatgcggt cacgctcctg
    21781 cttcacttcc cacaactctt tatgccccat gatcaccgta tccagcacag tgaactcttc
    21841 aaaggcaaac tgatcctgac gcagcttacc aatgcgctcg ttgggatcga gggaaacgtt
    21901 acccaaagtc ggctctaagt cgccgccgag gatcttcata aaggtggatt taccactacc
    21961 gttcgcgcca atcaggccgt aacggttgcc gccgccgaat ttgacggaaa tgttttcaaa
    22021 caacggctta ctgccgaact gcatggtgac gttactggaa actaacacag gcgtatcctg
    22081 aaaagagata tgacaaaccg cgcattatgc cataagtctg aaagaagatc ctgctcctcg
    22141 cttaaggtct acactcaagc caaaggcaaa aaatgtgatt tcgtacacat ctgatttcac
    22201 tgtgagctgg aatgaactta taatgcgctt ccaatactct aatattctca acccaatggc
    22261 ctgccaggca caaaatctcg cttaacatga atatgaaatt gaaaacatta ttcgcagcgg
    22321 ccttcgctgt tgtcggcttt tgcagtaccg cctctgcggt aacttatcct ctgccaaccg
    22381 acgggagtcg cctggttggt cagaatcagg tgatcaccat tcctgaaggt aacactcagc
    22441 cgctggagta ttttgccgcg gagtaccaga tggggctttc caatatgatg gaagcgaacc
    22501 cgggtgtgga taccttcctg ccgaaaggcg gtactgtact gaacattccg cagcagctga
    22561 tcctgccgga taccgttcat gaaggcatcg tcattaacag tgcagagatg cgtctgtatt
    22621 actatccgaa agggaccaac accgttatcg tgctgccgat cggcattggt cagttaggca
    22681 aagatacgcc tatcaactgg accaccaaag ttgagcgtaa gaaagcaggc ccgacctgga
    22741 cgccgaccgc caaaatgcac gcagagtacc gcgctgcggg cgaaccgctt ccggctgtcg
    22801 ttccggcagg tccggataac ccgatggggc tgtatgcact ctacatcggt cgcctgtacg
    22861 ctatccatgg caccaacgcc aacttcggta tcggcctgcg tgtaagtcat ggttgtgtgc
    22921 gtctgcgtaa cgaagacatc aaattcctgt tcgagaaagt accggtcggt acccgcgtac
    22981 agtttattga tgagccggta aaagcgacca ccgagccaga cggcagccgt tatattgaag
    23041 tccataaccc gctgtctacc accgaagccc agtttgaagg tcaggaaatt gtgccaatta
    23101 ccctgacgaa gagcgtgcaa acagtgaccg gtcagccaga tgttgaccag gttgttcttg
    23161 atgaagcgat taaaaaccgc tccgggatgc cggttcgtct gaattaatct tcaaaactta
    23221 aagcaaaagg cggactcata atccgccttt tttatttgcc agaccctagt tggccgggag
    23281 tataactaac aaaacatatc ccaccaacgc cgcccacaac agcatcggta ttgaatagag
    23341 atggaaacgc caccagatgc ggcgatcgtt cgccatacgt agcgcaatca aatttgccag
    23401 cgatccgggt aataacccaa agccacctac gtttaccgcc cataccagta ataaagacgg
    23461 cggcacatag ttcagcaaca atatggtact cggcacgtta ctgatcacct gcgataaacc
    23521 gattgccgtt aaccataacc cgggttcaga tagatgactc acgttaccca acacgccttg
    23581 caacgctgga agctgggtca gtaaatggac gtcgataaac atcgccataa acaccagcag
    23641 cagcgtccag tccacactga gcaccacgcg acgtgccagc agcgcaaagc ctgccgccac
    23701 aatcaccagt ccccacagct cttgtttgaa ctccagcgcc gtcagaaaga cgatatacag
    23761 ccccagacaa ctccacacca gccgcggttt ccactccggt gtttgcaccc ccgtatggta
    23821 ttgcagcgcc tttccaggga aacaacacca gcacaggagc aggagcgtca gcatcattgc
    23881 gccagccagc ggtgccattt gggcaataaa tccggcaaac gaaagaccag aacgtcccca
    23941 gataagaata ttttgcgggt tgccaattgg cgtcagtagc gaaccagcgt tgactgccag
    24001 cgcctcaaaa ataatcagcc gattaaccgg gatctcacac agtcttttta gcgtgatagt
    24061 cagcggaaca acaataaaca gcgcgacatc gttggtcaga aaggtagaaa gcagcgccgc
    24121 cgccagcacc ataaacatcg ccagccgacg ctccgtagca aagcggcgca ccattttgcg
    24181 ccccagcaca tcaaaataac cgcttaactc cacacctttg gtcagcagca tcaggccgct
    24241 taaggtgatg atggtgtgcc agtcgatagc agcaggccag gatttcggtg caaagggcac
    24301 gaaaaagctt aatccgatac caacaagaat taataactga aaaaaacgat cgccttgcag
    24361 cgtgcgtaaa aaaggcaggc tcatttggca ccgtgtttct gggtaaacaa acgaaaagcg
    24421 tcgagcgtct cttcactaac atggtgctcc atgccttccg cgtcgcgacg ggcgatttcc
    24481 ggactgacgc ccaacaccaa caagaaattt tcgactatct gatgtcgctc gcggctttcc
    24541 tgcgccagct tctctccttc tgccgttaaa aacacgccac gccaggggat catttcaatc
    24601 agccccatgg ttgccagccg cttaagcatt ttagccaccg tcggttgcga aactcccaga
    24661 cgagcagcca tgtccacctg acgagcttcc cccacttccc tgatcaagtc agaaatcagc
    24721 tcaacgtaat catcaataag ctcgcgccga tgcgcctctc tgacctggcg gaacccttca
    24781 acgtgctctt ccacgttcac taactgcgtc actttttttg ctgttggcgt acctgcgcga
    24841 cgactcattg tgcttcctca ttatggtgac gcatacatag cgttctcatt tcgtgcgcac
    24901 attgtaaacc agagttgcga aggtacaaaa aattaacgtt ttagcaatag ctacataata
    24961 tagcctgtgc tatatctgta tgtaatgcaa tcatccctca aggatcgacg ggattagcaa
    25021 gtcaggaggt cttatgaatg agttcaagaa gtgtatgcgc gtgtttagtc attctccctt
    25081 taaagtacgg ttaatgctgc tctctatgtt gtgcgatatg gtcaacaaca aaccgcagca
    25141 agataaacct tccgataaat agcggcgtcg cggtacgccg cttcactcct gcttttatgc
    25201 aggcataacg cgttttggtc tgaaaaaccc cactttttgt cggatttgca atccccttcg
    25261 caaaagattt gttcgtcagt agttgacctg aacggcggct cgctctatct tcttgcagcc
    25321 ctacgtatat tgcggctcgc ggatgcggac ccctttcccc tcttcacgca ctcttgcagg
    25381 tattgaccct tgacgccagg gtaagcacat ggcgtttgtt acgatagtgg catatgaatt
    25441 taaccctcaa agaatcgctt gttacccgta gccgggtatt tagcccgtgg actgcgttct
    25501 actttttaca gtcgctatta attaacctcg gcttaggtta ccccttcagt ttgctctaca
    25561 ccgctgcgtt tacggctatt ttgcttttgc tatggcgaac attgcctcgc gtacaaaaag
    25621 ttctggtcgg tgtcagttcg ctggtggcgg cttgttattt cccttttgct caggcctacg
    25681 gcgcgcctaa tttcaataca ttgctggcat tgcactccac caatatggaa gagtcgaccg
    25741 aaatcctgac gatttttccg tggtacagct acctggtcgg cttatttatt tttgcgctcg
    25801 gcgtaatagc aatcaggcga aaaaaagaga atgaaaaagc gcgctggaat accttcgaca
    25861 gcctgtgtct ggtattcagt gtggcgacat tttttgttgc tcccgtgcaa aacctggcct
    25921 ggggtggcgt atttaaactg aaagatactg gctatccggt atttcgtttt gctaaggatg
    25981 tcatcgtcaa taataacgag gtgattgaag agcaagaacg gatggcaaaa ctttccggaa
    26041 tgaaagatac ctggacggtc actgccgtta agccgaagta tcagacctat gtggtggtga
    26101 tcggcgaaag cgcgcgtcgc gatgccctcg gtgcctttgg cggtcactgg gacaataccc
    26161 cgtttgccag cagcgttaac ggtttgatat ttgctgacta cattgccgcc agtggctcca
    26221 cgcagaaatc acttggctta acgctcaatc gcgtagtcga tggcaaacca cagtttcagg
    26281 ataactttgt caccctggca aatcgcgcgg gcttccagac ctggtggttt tccaaccagg
    26341 gccaaatcgg cgaatacgat actgctatcg ccagcatcgc caaacgtgca gatgaagtgt
    26401 acttcctgaa agaaggtaat ttcgaagcag ataaaaacac gaaagacgaa gcgttactgg
    26461 atatgaccgc tcaagtgctg gcgcaagagc actcgcaacc gcagctgatt gttctgcatc
    26521 tgatgggctc gcatccgcag gcctgcgaca ggacacaagg caaatacgaa acctttgtgc
    26581 aatcgaaaga aacgtcgtgc tatctctata ccatgacgca aacggacgat ttactgcgca
    26641 agctgtacga tcagttacgc aacagcggca gcagcttctc tctggtttac ttttctgacc
    26701 acggtctggc ctttaaagag cgcggcaaag acgtgcaata ccttgcccat gatgataaat
    26761 atcagcaaaa tttccaggtg ccttttatgg tcatttccag cgacgataaa gcgcatcggg
    26821 tgattaaagc ccgccgctca gccaatgact tcttaggctt tttctcgcag tggacgggaa
    26881 ttaaagcgaa ggaaataaat atcaaatacc cgtttatatc tgagaagaaa gccgggccga
    26941 tatacatcac caacttccag ttacagaagg tggattacaa ccatctcgga accgatattt
    27001 tcgacccgaa accttaaaac aaaaaatccg ccccgagagg cggatttttt atatcaccaa
    27061 agtgattaga agcggtaacc aacaccggca atccaggtgc ctacgtcaac gctacgaata
    27121 cggctctgct cgtaagagaa gtccagagca acgttttcca tcgggttgaa ctgcagacca
    27181 gcaccgtagg agaaaccgta gtcgctggtg tcgtgtttgt aggtcgggta ttcagtggtc
    27241 tggaatttac cataacccac ccccactaca ccgtagatgc ttgcccagtc gttaatgcgg
    27301 taagccggac cagcagtgat gccgtagtac tggtttttgt tgtagtcacc agagcttgca
    27361 gtacggcttt tctcggtgta agtgaaagaa ccgatcacac ccagcgggct gttgtcttct
    27421 tcatagcggt atttcaggtt gaaaccgccc attttgttca tttggccctg agcgtcgctc
    27481 tgtgcgtaac cgccagttac ggtagaagtc gcagctacgg aagtacctgc ggtgaaagcc
    27541 agaactgcgg ccagtgctga aagacatgca atttttttca taaccacctc aaatgtgatt
    27601 caaataagtc ctaagtttta aatatatcaa aaattaatgg gaaactcttc gcgatttgtg
    27661 atgtctaacg ggccatttca tgtaacagaa cgtttccata caccgctatc catctaaatt
    27721 taaatcactt tttcagagaa ctgcgtaagt attacgcatg ttttccctgt cattcatcca
    27781 gattattcct aatcaccaga ctaatgattc catcaatcct ggcgcatttt agtcaaaacg
    27841 ggggaaaatt ttttcaacaa atgctcaacc agcattgggt atatccagta cactccacgc
    27901 tttacttaag tctagatatt tgtgggagaa aggatgcctg gttcattacg taaaatgccg
    27961 gtctggttac caatagtcat attgctcgtt gccatggcgt ctattcaggg tggagcctcg
    28021 ttagctaagt cactttttcc tctggtgggc gcaccgggtg tcactgcgct gcgtctggca
    28081 ttaggaacgc tgatcctcat cgcgttcttt aagccatggc gactgcgctt tgccaaagag
    28141 caacggttac cgctgttgtt ttacggcgtt tcgctgggtg ggatgaatta tcttttttat
    28201 ctttctattc agacagtacc gctgggtatt gcggtggcgc tggagttcac cggaccactg
    28261 gcggtggcgc tgttctcttc tcgtcgcccg gtagatttcg tctgggttgt gctggcggtt
    28321 cttggtctgt ggttcctgct accgctgggg caagacgttt cccatgtcga tttaaccggc
    28381 tgtgcgctgg cactgggggc cggggcttgt tgggctattt acattttaag tgggcaacgc
    28441 gcaggagcgg aacatggccc tgcgacggtg gcaattggtt cgttgattgc agcgttaatt
    28501 ttcgtgccaa ttggagcgct tcaggctggt gaagcactct ggcactggtc ggttattcca
    28561 ttgggtctgg ctgtcgctat tctctcgacc gctctgcctt attcgctgga aatgattgcc
    28621 ctcacccgtt tgccaacacg gacatttggt acgctgatga gcatggaacc ggcgctggct
    28681 gccgtttccg ggatgatttt cctcggagaa acactgacac ccatacagct gctggcgctc
    28741 ggcgctatca tcgccgcttc aatggggtct acgctgacag tacgcaaaga gagcaaaata
    28801 aaagaattag acattaatta aatttacatt tctgcatggt tatgcataac catgcagaat
    28861 ttctcgctac ttttcctcta caccgtcttt atatatcgaa ttatgcaaaa gcatatttat
    28921 tccgaaaatt cctggcgagc agataaataa gaattgttct tatcaatata tctaactcat
    28981 tgaatcttta ttagttttgt ttttcacgct tgttaccact attagtgtga taggaacagc
    29041 cagaatagcg gaacacatag ccggtgctat acttagtctc gttaattact gggacataac
    29101 atcaagagga tatgaaatta tgagtaccgc taaattagtt aaatcaaaag cgaccaatct
    29161 gctttatacc cgcaacgatg tctccgacag cgagaaaaaa gcaacagtag agttgctgaa
    29221 tcgccaggtt atccagttta ttgatctttc tttgattacc aaacaagcgc actggaacat
    29281 gcgcggcgct aacttcattg ccgtacatga aatgctggat ggcttccgca ccgcactgat
    29341 cgatcatctg gataccatgg cagaacgtgc agtgcagctg ggcggtgtag ctctggggac
    29401 cactcaagtt atcaacagca aaaccccgct gaaaagttac ccgctggaca tccacaacgt
    29461 tcaggatcac ctgaaagaac tggctgaccg ttacgcaatc gtcgctaatg acgtacgcaa
    29521 agcgattggc gaagcgaaag atgacgacac cgcagatatc ctgaccgccg cgtctcgcga
    29581 cctggataaa ttcctgtggt ttatcgagtc taacatcgaa taaatccatc gctgatggtg
    29641 cagaacttta gtacccgata aaagcggctt cctgacagga ggccgttttg ttttgcagcc
    29701 cacctcaacg cacttattta gtgcatccat ctgctatctc cagctgatta agtaaatttt
    29761 ttgtatccac atcatcacac aatcgttaca taaagattgt tttttcatca ggttttacgc
    29821 taaataatca ctgtgttgag tgcacaattt tagcgcacca gattggtgcc ccagaatggt
    29881 gcatcttcag ggtattgccc tataaatcgt gcatcacgtt tttgccgcat ctcgaaaaat
    29941 caaggagttg caaaactggc acgatttttt catatatgtg aatgtcacgc aggggatcgt
    30001 cccgtggata gaaaaaagga aatgctatga agtctgtatt aaaagtttca ctggctgcac
    30061 tgaccctggc ttttgcggtt tcttctcatg ccgcggataa aaaattagtt gtcgcgacgg
    30121 ataccgcctt cgttccgttt gaatttaaac agggcgataa atatgtgggc tttgacgttg
    30181 atctgtgggc tgccatcgct aaagagctga agctggatta cgaactgaag ccgatggatt
    30241 tcagtgggat cattccggca ctgcaaacca aaaacgtcga tctggcgctg gcgggcatta
    30301 ccatcaccga cgagcgtaaa aaagcgatcg atttctctga cggctactac aaaagcggcc
    30361 tgttagtgat ggtgaaagct aacaataacg atgtgaaaag cgtgaaagat ctcgacggga
    30421 aagtggttgc tgttaagagc ggtactggct ccgttgatta cgcgaaagca aacatcaaaa
    30481 ctaaagatct gcgtcagttc ccgaacatcg ataacgccta tatggaactg ggcaccaacc
    30541 gcgcagacgc cgttctgcac gatacgccaa acattctgta cttcatcaaa accgccggta
    30601 acggtcagtt caaagcggta ggtgactctc tggaagcgca gcaatacggt attgcgttcc
    30661 cgaaaggtag cgacgagctg cgtgacaaag tcaacggcgc gttgaaaacc ctgcgcgaga
    30721 acggaactta caacgaaatc tacaaaaaat ggttcggtac tgaaccgaaa taataacgct
    30781 acacctgtaa agcgcactgg cagttccctc tcccctctgg ggagaggatt agggtgaggg
    30841 gcgcaaaccc gctccggggc cattaattac cctgaatttg attatttaca ccacggtaac
    30901 aggaacaaca tatgcagttt gactggagtg ccatctggcc tgccattccg cttctgattg
    30961 aaggtgccaa aatgaccctg tggatttcgg tcctcggtct ggctggcggt ctggtaatcg
    31021 gattgctggc aggttttgca cgcaccttcg gtggttggat agccaaccac gtcgcgctgg
    31081 tctttattga ggtgatccgc ggcacaccta tcgtcgtcca ggtgatgttt atttatttcg
    31141 ccctgccgat ggcgtttaac gacttacgca tcgacccatt tactgcggcg gtggtcacca
    31201 tcatgatcaa ctccggcgcg tacattgcgg aaatcacgcg tggtgcggtg ctgtctattc
    31261 acaaaggttt tcgtgaagca ggactggcgc tcggtctttc acgttgggaa accattcgct
    31321 acgtcatttt accgctggca ctgcgtcgta tgctgccgcc gctgggtaac cagtggatca
    31381 tcagcattaa agacacctcg ctgtttattg tgatcggcgt ggcggaactg acccgtcagg
    31441 ggcaagaaat tattgccggt aacttccgcg cccttgagat ctggagcgcc gtggcggtgt
    31501 tctatctgat tattaccctg gtgctaagct ttattttgcg tcgtctggaa agaaggatga
    31561 aaatcctgtg attgaattta aaaacgtctc caagcacttt ggcccaaccc aggtgcttca
    31621 caatatcgat ttgaacattg cccagggcga agtcgtggtg attatcgggc cgtccggttc
    31681 cggtaaatcg accctgctgc gctgcatcaa caaactggaa gaaatcacct ccggcgatct
    31741 gattgtcgat ggcctgaagg ttaacgatcc gaaagttgac gagcgcctga ttcgccagga
    31801 agcaggtatg gtgttccagc agttttacct cttcccgcat ctgacggcgc tggaaaacgt
    31861 catgtttggt ccgctacgcg tgcgtggcgc gaacaaagaa gaggcggaaa aactggcgcg
    31921 tgagctgctg gcgaaagtcg gtctggcaga acgtgcacat cactaccctt ccgaactttc
    31981 tggtggtcaa cagcagcgtg tggcgattgc ccgcgcgctg gcggtgaagc cgaaaatgat
    32041 gctgtttgat gaaccgactt ccgctcttga cccggaactg cgccatgaag tgctgaaggt
    32101 tatgcaggat ctggctgaag aagggatgac gatggtgatc gtgacccacg aaatcggttt
    32161 tgccgagaaa gtagcttcgc ggctgatctt tatcgacaaa ggccggattg cggaagatgg
    32221 cgatccgcag gtattgatca agaacccgcc gagccagcgc ttgcaggaat ttttgcagca
    32281 cgtctcttaa cttctctgcc tccctttcac ccgaaaggga ggcacaccag attcctctca
    32341 tttaaaatcg cccctccccc agcatctata cttatctttt tgctctgttt tctcactgga
    32401 ggagtcatgc ggtggatcct gttcatcctc ttctgcctgc tgggcgcacc tgcccacgcg
    32461 gtatccatac ccggcgttac aaccacaacg acaacggact caacgactga accggccccg
    32521 gaaccggata tcgaacaaaa aaaagcggcc tatggcgcac tggcggatgt gctggataac
    32581 gacacctcgc gtaaagaatt gatcgaccag ttgcgtactg tcgccgcgac gccccctgcg
    32641 gaaccggtcc cgaagatcgt gccgccgacg ctggttgaag agcaaaccgt gctgcaaaag
    32701 gtcaccgaag tcagccgcca ttatggtgaa gccctttccg cccgcttcgg gcaactttat
    32761 cgcaatatca ccggctcccc gcataagcca tttaatccac aaaccttcag caatgcgctg
    32821 agccattttt caatgttagc ggtattagtg tttggttttt actggctgat tcgcctgtgc
    32881 gcactgccgc tgtatcgcaa aatgggtcag tgggcgaggc aaaaaaatcg tgagcgcagc
    32941 aactggttgc agcttccggc gatgattatc ggggcgttta ttatcgacct gctgttactg
    33001 gccctgacat tatttgtcgg ccaggtatta agcgacaacc tgaatgcagg cagtcgcacc
    33061 atcgctttcc aacaaagttt gtttctcaac gcctttgccc tgattgaatt tttcaaagcc
    33121 gtactacgcc tgattttttg cccaaacgtg gcggagctgc gcccgttcac gattcaggac
    33181 gagagcgccc gttactggag tcgtcgcctg agctggttaa gcagtttgat aggttatggc
    33241 ctgattgtgg ccgtgccgat tatctctaat caggtgaatg tacagatagg tgcgctggcg
    33301 aacgtcatca ttatgctgtg catgaccgtc tgggcgttgt acctgatctt tcgtaataaa
    33361 aaagagatta cccagcattt gctcaacttt gcggagcatt cgctggcctt tttcagcctg
    33421 tttatccgcg cctttgcgct ggtgtggcac tggctggcaa gcgcctattt tatcgtgctg
    33481 tttttctttt cgttgttcga tccgggcaac agcctgaaat ttatgatggg tgcaacggtg
    33541 cgcagcctgg cgattattgg tatcgcagcg tttgtttccg gtatgttttc ccgctggctg
    33601 gcaaaaacca tcactctctc gccacatact cagcgtaact atccagagtt gcaaaaacgg
    33661 ctgaatggct ggctttcggc ggcgctgaaa acggcgcgta ttctgaccgt ctgcgtggcg
    33721 gtaatgttgc tgttgagcgc atggggattg ttcgatttct ggaactggct gcaaaacggc
    33781 gcggggcaga aaaccgtaga tatcctgatc cgtatcgcac ttattctttt tttctcggcg
    33841 gttggctgga cggtgctcgc cagtttgatc gaaaaccggc tggcttcgga tattcatggc
    33901 cgcccgctac ccagcgcccg tacgcgtacc ctgctgacgc tgttccgtaa cgcgctggcg
    33961 gtgattatca gtaccatcac catcatgatt gtgttgtcgg aaatcggcgt caatatcgcg
    34021 ccattgctgg caggtgccgg ggcattaggt ctggctatct cgtttggttc gcaaacgctg
    34081 gtgaaagata tcattaccgg ggtgtttatt cagtttgaaa acggcatgaa cactggagat
    34141 ttggtgacta tcgggccgtt gaccggcaca gtggaacgga tgtcgattcg ctccgtgggc
    34201 gtgcgccagg ataccggggc gtatcacatc attccgtggt cttcgataac cacctttgct
    34261 aacttcgtcc gcggtattgg ttcggtagtg gcaaactacg atgttgatcg ccatgaagat
    34321 gctgataaag ccaatcaggc actgaaagat gcggtagcgg aattaatgga aaacgaagaa
    34381 attcgcgggc tgattattgg tgaaccgaat tttgccggga ttgtcggctt aagcaatacc
    34441 gcgtttacac tgcgtgtttc gttcaccacg ctgccactca aacagtggac ggtacgcttt
    34501 gccctcgaca gccaggtgaa aaaacatttc gacctggcgg gcgttcgcgc gccagtgcag
    34561 acttatcagg tgctgcctac tccgggcgcg accccggctg aaccgttacc gccgggggag
    34621 ccaacgcttt aacgctggcg attgacaaaa cggcggcgct gctcgtcgtt cataaaggtc
    34681 caggcaataa agcgactctg cttttgcccc tgggccatct ctttttttac caccttcacc
    34741 gcgcccacgt cggtcagagc acgatacaac ggcggtaagt tttcaccacg agataccagc
    34801 gaggtaaacc acatcacctg cttcgcgaag cctttgctct cttcaatcat ctttttgata
    34861 aaggcgactt caccaccttc acaccacaac tcctgttgct gaccaccaaa gttcagtgca
    34921 tcgtctttgt tcagccccag gttacggcgt ttacgctcac tacctgcccg cgctgcagcg
    34981 gcggaatcgt ggaatggcgg gttacacaag gtcgcgtcgt attgctcgtt tttatggatg
    35041 atgccgttaa aaattgcccc actctctttt tgccgacgca gacgaatggc gcggttaagc
    35101 cccggattag cactgataat cgcctgcgca ctgcttaacg cctggctgct ggtttcacta
    35161 ccggtaaaac gccagccata ttcatgtacg ccaatcagcg gataaataca gttcgcacca
    35221 acgccgatat ccagaatgct ggcattcgct ggaattgttc cgcttgcttc agccagtaaa
    35281 tcggcaaggt gatgaatata atccgcccgg cccggtactg gtgggcagag aaaaccgtcg
    35341 gggatatccc agttcgctac ggcgtaaaaa tgggccagca acgccttatt gagcgccttc
    35401 accgccagcg gattggcaaa gtctacgctt tgctccccgg cgggtgtaag cgtgaggaat
    35461 tgcctgagtt caggattgac ctgacaaagc gtggcgagat cgtagcggct gtgatgacgg
    35521 ttgcgcggat gcaaccccgg tttctgggcg gacatagcat tctcctttaa gcagcggcgt
    35581 aagatacccg cataacgcga cagggtaaat atctggctgc gtttatcatt ccccaaaacc
    35641 ttaacatggc tatggtttat ggcaattcat cataaaaatc agcattatgc atgtcattaa
    35701 tttgtcttat tttgtaaaaa tatatcgttc aaaaaataag cacatctctg tcaatttttt
    35761 gcgtcatttg catcgcgcag gcgacctaca cttatcaggc actacccgtt gtatttatga
    35821 ggatgtcatt atgaaaaagt gcctcacact actgattgcc accgtcctga gcggaatctc
    35881 tctcacggct tatgccgcgc aaccgatgag taacctggac agcggtcagc tacggcccgc
    35941 cggtaccgtt tcggcaactg gcgcatcaaa cctaagcgat ctggaggata aactggcaga
    36001 aaaagcgcgc gaacaaggcg cgaaaggtta tgtcattaat tccgcaggcg gaaatgacca
    36061 gatgttcggt actgcaacca tctacaaata accccaccaa cgtaactgca ttcgcctctg
    36121 gatgcagttt tcgcctgttt tgtaatcaaa aaacatcaaa tatgaactca atgtaaataa
    36181 atgtatttct ttttcgcgca atgggtgata gaaaatcgcg ccaagtgata atgcttatca
    36241 aaattattat cacttgcatg aacactatca cgggattaac agtggcatcg catccgcaga
    36301 gatgctttct cgtggcagtg aaaatttcaa catataagaa aaagtcacct gcaaaatgga
    36361 aaacaatcgc aatttccctg ccagacaatt tcattcgctc acgttctttg ccggtctttg
    36421 tattggcatc acgcctgtgg ctcaggcact cgccgccgaa gggcaaacta acgcggatga
    36481 cacgctggtt gtcgaagcat caacgccttc gctttatgcg ccacaacaat ctgccgatcc
    36541 gaaattctcg cgtccggtag cggatactac ccgcacgatg acggtgattt ctgaacaagt
    36601 gattaaagat cagggcgcaa ccaaccttac cgacgcgctc aaaaacgccc ccggcg
//
LOCUS       DAJVQG010000023        35779 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJVQG010000023 DAJVQG010000000
VERSION     DAJVQG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.023"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(1..581)
                     /locus_tag="OQM83_001057"
                     /pseudo
     CDS             complement(1..581)
                     /locus_tag="OQM83_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(810..1730)
                     /gene="malM"
                     /locus_tag="OQM83_001058"
     CDS             complement(810..1730)
                     /gene="malM"
                     /locus_tag="OQM83_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCS1519374.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVSYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="OQM83_001059"
     CDS             complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="OQM83_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCS1519375.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(3294..4409)
                     /gene="malK"
                     /locus_tag="OQM83_001060"
     CDS             complement(3294..4409)
                     /gene="malK"
                     /locus_tag="OQM83_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCS1519376.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4774..5964
                     /gene="malE"
                     /locus_tag="OQM83_001061"
     CDS             4774..5964
                     /gene="malE"
                     /locus_tag="OQM83_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCS1519377.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            6118..7662
                     /gene="malF"
                     /locus_tag="OQM83_001062"
     CDS             6118..7662
                     /gene="malF"
                     /locus_tag="OQM83_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCS1519378.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGADPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            7677..8567
                     /gene="malG"
                     /locus_tag="OQM83_001063"
     CDS             7677..8567
                     /gene="malG"
                     /locus_tag="OQM83_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCS1519379.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8939..10414
                     /gene="xylE"
                     /locus_tag="OQM83_001064"
     CDS             8939..10414
                     /gene="xylE"
                     /locus_tag="OQM83_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCS1519380.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VPLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="OQM83_001065"
     CDS             complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="OQM83_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCS1519381.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(10994..11137)
                     /locus_tag="OQM83_001066"
     CDS             complement(10994..11137)
                     /locus_tag="OQM83_001066"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1519382.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            11138..11416
                     /locus_tag="OQM83_001067"
     CDS             11138..11416
                     /locus_tag="OQM83_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1519383.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11463..13559)
                     /locus_tag="OQM83_001068"
     CDS             complement(11463..13559)
                     /locus_tag="OQM83_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCS1519384.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13559..14296)
                     /locus_tag="OQM83_001069"
     CDS             complement(13559..14296)
                     /locus_tag="OQM83_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCS1519385.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(14293..14931)
                     /locus_tag="OQM83_001070"
     CDS             complement(14293..14931)
                     /locus_tag="OQM83_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCS1519386.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(15045..15287)
                     /locus_tag="OQM83_001071"
     CDS             complement(15045..15287)
                     /locus_tag="OQM83_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1519387.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="OQM83_001072"
     CDS             complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="OQM83_001072"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCS1519388.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            17923..19272
                     /gene="lysC"
                     /locus_tag="OQM83_001073"
     CDS             17923..19272
                     /gene="lysC"
                     /locus_tag="OQM83_001073"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCS1519389.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            19524..19796
                     /locus_tag="OQM83_001074"
     CDS             19524..19796
                     /locus_tag="OQM83_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCS1519390.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="OQM83_001075"
     CDS             complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="OQM83_001075"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCS1519391.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            21149..21986
                     /locus_tag="OQM83_001076"
                     /pseudo
     CDS             21149..21986
                     /locus_tag="OQM83_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            22056..22862
                     /locus_tag="OQM83_001077"
     CDS             22056..22862
                     /locus_tag="OQM83_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1519392.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            22872..23279
                     /locus_tag="OQM83_001078"
     CDS             22872..23279
                     /locus_tag="OQM83_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCS1519393.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            23279..23773
                     /locus_tag="OQM83_001079"
                     /pseudo
     CDS             23279..23773
                     /locus_tag="OQM83_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            23839..24636
                     /locus_tag="OQM83_001080"
     CDS             23839..24636
                     /locus_tag="OQM83_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCS1519394.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            24647..25471
                     /locus_tag="OQM83_001081"
     CDS             24647..25471
                     /locus_tag="OQM83_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCS1519395.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            25523..26751
                     /gene="sorE"
                     /locus_tag="OQM83_001082"
                     /pseudo
     CDS             25523..26751
                     /gene="sorE"
                     /locus_tag="OQM83_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            27134..27824
                     /gene="pepE"
                     /locus_tag="OQM83_001083"
                     /pseudo
     CDS             27134..27824
                     /gene="pepE"
                     /locus_tag="OQM83_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
     gene            complement(27915..29546)
                     /locus_tag="OQM83_001084"
     CDS             complement(27915..29546)
                     /locus_tag="OQM83_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCS1519396.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(29766..33449)
                     /gene="metH"
                     /locus_tag="OQM83_001085"
     CDS             complement(29766..33449)
                     /gene="metH"
                     /locus_tag="OQM83_001085"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCS1519397.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     VAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            33649..34473
                     /gene="iclR"
                     /locus_tag="OQM83_001086"
     CDS             33649..34473
                     /gene="iclR"
                     /locus_tag="OQM83_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCS1519398.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            34790..35779
                     /gene="espL4"
                     /locus_tag="OQM83_001087"
                     /pseudo
     CDS             34790..35779
                     /gene="espL4"
                     /locus_tag="OQM83_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
BASE COUNT         8598 a         9232 c         9102 g         8847 t
ORIGIN      
        1 actatgacca agttgactct gcgagaagtc aactgtatca agaatatttt ggctaaaatc
       61 aacacattcg ccactcgttt tgagaaaaat cacattgtgt aaacgtcgac atttaaatat
      121 gacattatca actgttgcct tagtaaaatt gcaattctca atttctttac attcgaaagt
      181 tacatgttgt agatttgatg ataaaaaaga caccttagaa ataacgtcac ctttaaaatg
      241 acaatttatt aaatcaaaat cagacaggtc gatatacccc tgcgcatctt tatccatcag
      301 aaaattacaa gcgcattcta ttttttcatt tctgtagaat tttacactta tagtatcatt
      361 actaattgag acttcacgta acatatttat tgcatgattt tttcgttccg ttgctttata
      421 tttaaaaata tatcactgat ataaataaag ttatctctgt ttaaacctgt caccccattg
      481 aaattacgta gtaaatcaag atctactata ttatttcctt gaaccacatc atgtttcatc
      541 aaagagttat tgccgccaga atttgtgata tgacctatca taaaacctct gtaatacaga
      601 aataaaataa cctggaaaag tagcctgagc gtccagacac taccggcgaa aggaaaagaa
      661 tctctgataa ggcattgaga taatggacat tcttagtgaa gaagaagtga agtactataa
      721 aaaatcgctc atatcccagg aaggaagcat aaaaaaaggt gcgccaggag acgcaccagt
      781 tgttgcaaaa tcagcactgt ggggcgtaat taccccttgc cttttacact gctgataaag
      841 gtggaacggg cagatgtcga tcccaggcgt tcagcttcat caagcagttt taacgcctta
      901 tcaacatcac ctttcgcgac agcgtttttg atcgcggtat taaaataact ttccgtgtcg
      961 ttgagcatcg gctcgctttt cttcaccggc accggagcag gtgcagccac agctggtgcc
     1021 gccgtgttgc ctaccgtaac cggagctgga gcggaagaac caaatagtgg tcctaccaac
     1081 acgctggagc tggagttcgt tttgactttc agtttcagta agccatcgct ggtatgacga
     1141 gcaaccggat cgggaatatc cgggatcgag ttaccgacgc ctttggcata ggctttagcc
     1201 gggtcgagca gttgggtcgt ctgctggaga tctttttccg tggtaaagac cagaacataa
     1261 agtttttgct gccccaacgc cggtgtcagg cgcataacgc cttccagccg atctgcactc
     1321 atcacgcctg gttcctggta ggtgaaataa ctgctgggga agaaggctga tggggtcatg
     1381 ttctgatcaa gaatcagcac gttcggcgca aaaacgctgg tttgtttgtt cacttcgctg
     1441 gtcagcgtca gggtcagttc gccaatgttt gccgggacgc tgtacgcagc aaccggacca
     1501 ctgatgccgg gaacgttcag ttgttggccg ccggtcgcca gttgggtggt ctgggtttta
     1561 gattgatcga ccggtgtcca ggtgagttgt tgcagcgcgg cagatggaat ggctggcgcg
     1621 tcgctggtgt tttgcggtac gtagctaaca tcggcaaggc taattccagg cgcgcttgcc
     1681 agtaaccctg ctgataaaca gagggcgatg agacttttat tcattttcat tgttttcacc
     1741 tcaaaatctg gagctcagcg gtagccaggc aatagcgcgc taaacccgat aatcagaggg
     1801 gctttcgccc ctttcagata atgacaacct gtttttatgc cggatgcggc gtaaacgcct
     1861 tatccggccc aggttttgct attaccacca gatttccatc tgggcaccga aggtccactc
     1921 gtcgctgtcg ccacgaccga agctgccgcc gttgaaatca gcaggaacgg ctttgccgta
     1981 gttcgggtta accttgctat cgccgttgta gtcgtaaccc catttctcat cccacttggc
     2041 gtaggttgcg aagacacgaa tagccgggcg tgaccagatg ctgtcgccag cctgccattg
     2101 ttgtgcgagg gtaattttgt actgattgtt cttgtcgccg gtgcgctggg attcgacgtt
     2161 gtcgtagccg atttccatca cggtgctcat gattggcgtc cacttgtaca tcgggcgaat
     2221 accgacggtc caccacttgg tgccgttgtc gttatcccag ttgatatcct ggtacatacc
     2281 cacgtacatc atgtcccagt tgtcgcccat ggagatcgca ccgtggtcga ggatacgcag
     2341 catgtgaccg ttgttgttga tattgtaggc aaatttttcg ttatcaaacg cgacgccaga
     2401 accctgcgac agacctttac cctgcgaggt catcgagtca gtagcgtact gaacaacaaa
     2461 cttgttaaag cccttcagga cactctgagt atgttcagca gtgaataacc agccgtcttt
     2521 cgatgcgcca tcaaccagac gatagttatc acgcaggttg gcacgaccgt agtcgacacc
     2581 cagttctaat gtgccgcccg ggttgatttc catctgcgct aaacgcacat cgaaaacgtc
     2641 gttcgcggtt tcgttggtat agtcataaat attgttgctg gcaaaagagg aagaaccacc
     2701 agcttcagag gagcgggttg ctgccagaga gagtttaccg aagccaacat cgatgttttc
     2761 cagaccggca ccaggaccag aaatatccca gtagtagaag tcgatcatat gaacgtcatg
     2821 acgttggtag aagcgcttac ctgcccagat ggtggaacct ggcagccatt cgatcaggtt
     2881 tttaccctgc acgtttgctt cacggaaggc cggatcggta gcttcccagt cattctgttg
     2941 cgcgacggaa taggccacgt tagtgtcgaa atagaagctc ttatcgccct ctttccacac
     3001 ttcctgaccc aattttaatt cagcataagt ttcacattcg ttgccaagac ggtatttact
     3061 ttgagcaccg gtagtctgga aacactgttg ttcaccgccg ctacctgtcc agccaatacc
     3121 ggaacgtgca tagccgtgga aatcaacagc cattgcctga gcagacatta cgcccgctgc
     3181 gacggcaacc gccagaggaa gtttgcgcag agtaatcatc attctatctc ctgagtcatt
     3241 gcttttcttt tttcacatca cctgtgacag gctttgtgtg ttttgtgggg tgcttaaacg
     3301 cccggctcct tatgcagtcg acgacatgca gtgccatcct cacggaacag atggcaacgc
     3361 tctggcggca ggccgatagc gaatgtggca ccttcttcta ccaacaccac gtcgttctgg
     3421 cggtacacca ggttttgacg aatggaaggg atctggatat ggatttgggt ttcgttgccg
     3481 agttgctcga cgacctgaac ttcaccctca aggatgacgt cagcgatatc actcggcagt
     3541 agatgttccg ggcgaatacc cagcgacata ttggctccaa cctggacatc acggctttca
     3601 actggcagcc agacttgctg acgatttggc atcggcagct ccacctgcac ttgatcgatt
     3661 gcggtggcgg tcacttttac cggcaggaag ttcatctttg gcgaaccgat aaatccggcg
     3721 acaaaacggt ctgccggata gtggtacagc tccagcggtt tcccaacctg cgccacgcga
     3781 ccggcgtcca gcaccacgat tttgtcggcc agcgtcatcg cttcgacctg atcgtgggtg
     3841 acgtaaatca ttgtgcggcc caggcgttta tgcagacggg agatttcgat acgcatttgc
     3901 acacgcagtg cagcatcgag gttggagagc ggttcatcga gcaaaaatac gcttggctcg
     3961 gccaccagcg tacggccaat cgccacacgc tgacgctgac caccggagag cgctttcggt
     4021 ttgcgatcca gcaaatgcgc cagttgcagc acttccgcca cctggttgac gcgttggtta
     4081 atcacctctt tttttgcgcc agccagtttc aggccaaatg acatgttttc tgctactgac
     4141 aggtggggat agagcgcgta agactgaaac accataccaa cgccgcgttc tgctggcgga
     4201 gtgtcattca tccgtttctc accgatgaac aggtcgccgc tggtgatcgt ctcaagcccg
     4261 gcaatcatgc gcagtaaagt cgatttaccg cagccagacg gtccgacaaa caccacgaat
     4321 tcaccttcat ggatatcgag attgatatct ttcgatacca cgacctcgcc ccaggctttc
     4381 gttacatttt gcagctgtac gctcgccatg cccttctccc tttgtaacaa cctgtcatcg
     4441 acagcaacat tcatgatggg ctgactatgc gtcatcagga gatggcttaa atcctccacc
     4501 ccctggcttt tttatggggg aggaggcggg aggatgagaa cacggcttct gtgaactaaa
     4561 ccgaggtcat gtaaggaatt tcgtgatgtt gcttgcaaaa atcgtggcga ttttatgtgc
     4621 gcatctccac attaccgcca attctgtaac agagatcaca caaagcaacg gtggggcgta
     4681 ggggcaagga ggatggaaag aggttgccgt ataaagaaac tagagtccgt ttaggtgttt
     4741 tcacgagcac ttcaccaaca aggaccatag attatgaaaa taaaaacagg tgcacgcatc
     4801 ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa
     4861 gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc
     4921 ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg
     4981 gaagagaaat tccctcaggt tgcggcaact ggcgatggcc ctgacattat cttctgggcg
     5041 cacgaccgtt ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa
     5101 gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg
     5161 attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg
     5221 aacccaccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt
     5281 aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct
     5341 gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg atattaaaga cgtggccgtg
     5401 gataacgctg gcgcgaaagc gggtctgacc ttcctggttg accttattaa aaacaaacac
     5461 atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg
     5521 atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt
     5581 gtaacggtac tgccgacctt caagggtcaa ccatctaaac cgttcgttgg cgtgctgagc
     5641 gccggtatta acgccgccag tccgaacaaa gagctggcga aagagttcct cgaaaactat
     5701 ctgctgactg acgaaggtct ggaagcggtc aataaagaca aaccgctggg tgcagtggcg
     5761 ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac
     5821 gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg
     5881 cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac
     5941 gcgcagactc gtatcaccaa gtaatgctgt gaaatgccgg atgcggcgtg aacgccttgt
     6001 ccggcctaca aaactgaaac gcatgtaggc ctgataagac gcatcagtgt cgcatcaggc
     6061 agttgttgtc ggataaggcg tgaaagcctt atccgtcctg gaatgaggaa gaaccccatg
     6121 gatgtcatta aaaagaaaca ttggtggcaa agcgacgcgc tgaaatggtc agtgctaggt
     6181 ctgctcggcc tgctggtggg ttaccttgtt gttttaatgt acgcacaagg ggaatacctg
     6241 ttcgccatta ccacgctgat attgagttca gcggggctgt atattttcgc caatcgtaaa
     6301 gcctacgcct ggcgctatgt ttacccggga atggctggaa tgggattatt cgtcctcttc
     6361 cctctggtct gcaccatcgc cattgccttc accaactaca gcagcactaa ccagctgact
     6421 tttgaatgtg cgcaggaagt gttgttagat cgctcctggc aagcaggcaa aacctataac
     6481 tttggtcttt acccggcggg cgatgagtgg caactggcgc tcagtgacgg cgaaatcggc
     6541 aaaaattacc tctccgacgc gtttaaattt ggcggcgaac aaaaactgca actgaaagaa
     6601 acgaccgccc agcccgaagg cgaacgcgcg aatctacgcg tgattaccca gaatcgtcag
     6661 gcgctgagtg acattaccgc cattctgccg gatggcaaca aagtgatgat gagctccctg
     6721 cgccagtttt ctggcacgca gccgctctac acactcgacg gtgacggcac gttgacgaat
     6781 aatcagagcg gcgtgaaata tcgtccgaat aaccaaattg gcttttacca gtccattacc
     6841 gccgacggca actggggtga tgaaaagcta agccccggtt acaccgtgac tactggctgg
     6901 aaaaacttta cccgcgtctt caccgacgaa ggcattcaga aaccgttcct cgccattttc
     6961 gtctggaccg tggtgttctc gctgatcact gtctttttaa cggtggcggt cggcatggtt
     7021 ctggcatgtc tggtgcagtg ggaagcattg cgcggcaaag cggtctatcg cgttctgctg
     7081 attctgccct acgcggtgcc atcattcatt tcaatcttga ttttcaaagg gttgtttaac
     7141 cagagcttcg gtgaaatcaa catgatgttg agcgcgctgt ttggcgtgaa gcccgcctgg
     7201 ttcagcgatc cgaccaccgc ccgcacgatg ctaattatcg tcaatacctg gctgggttat
     7261 ccgtacatga tgatcctctg catgggcttg ctgaaagcga ttccggacga tttgtatgaa
     7321 gcctcagcaa tggatggcgc agatccgttc cagaacttct ttaagattac gctgccgctg
     7381 ctgattaaac cgctgacgcc gctgatgatc gccagcttcg cctttaactt taacaacttc
     7441 gtgctgattc aactgttaac caacggcggc ccggatcgtc ttggcacgac cacgccagcc
     7501 ggttataccg acctgcttgt taactacacc taccgtatcg cttttgaagg cggcgggggt
     7561 caggacttcg gtctggctgc agcaattgcc acgctgctct tcctgctggt gggtgcgctg
     7621 gcgatagtga acctgaaagc cacgcgaatg aagtttgatt aagggagata acaaaaatgg
     7681 caatggtcca accgaaatcg caaaaagcac gtttgtttat tactcacctg ctactgctac
     7741 tttttatcgc ggcgattatg ttcccgctgc tgatggtcgt cgctatctca ctgcgccagg
     7801 ggaactttgc gatcggcagc ctgatcccgg agcaaatctc ctgggatcac tggaaactgg
     7861 ctttaggttt tagcgtggaa caggctgatg gtcgcattac gccaccgcca ttcccggtac
     7921 tgctgtggct atggaactcg gtaaaggtcg ccgggatttc cgcgattggc attgtcgcgc
     7981 tctctaccac ctgcgcctac gctttcgccc gtatgcgctt tccaggcaaa gcgacgctgc
     8041 tgaaaggaat gctgattttc cagatgttcc cggcagtact ttcactggtc gcgttgtatg
     8101 cgttgtttga tcgtctgggt gagtacattc cattcattgg cctgaatact cacggcggcg
     8161 taattttcgc gtatctgggt gggattgcgc tgcatgtctg gaccatcaaa ggctatttcg
     8221 aaaccatcga tagttcgctg gaagaagcgg ctgcgctgga tggtgcgaca ccgtggcagg
     8281 ccttccgcct tgtcctgttg ccgctgtcag taccgattct ggcggtggta ttcatcctgt
     8341 cgtttatcgc tgccattact gaagttccgg tcgcgtcgct gttactgcgt gacgtaaaca
     8401 gctacaccct ggccgtgggg atgcagcaat acctcaaccc gcaaaactac ctgtggggtg
     8461 actttgccgc cgctgccgtg atgtctgcat taccgatcac catcgtcttc ttgctggctc
     8521 aacgctggct ggtcaacggc ctgacggcag gtggtgtgaa aggttaaaga tgttgttctg
     8581 ccaatgttat gccgctgcac cctcaactta cgttatccca acttgtgact gttattcggc
     8641 gctccacgga gcgccttttt ttctttcgtc tgcaatctga atcgttcgcc ggttaatatt
     8701 tccatcatag agcttattat ttttacgtta tttgttttcc cacttacgat aattctcttt
     8761 cgtgctctga gtcacggcaa tagtattgtt ttcatcaatt tttgataatt atcacaatta
     8821 agatcacaga aaagacatta cgtaaacgca ttgtaaaaaa tgataattgc cttaactgcc
     8881 tgacaattcc aacatcaatg cactgataaa agatcagaat ggtctaaggc aggtctgaat
     8941 gaatacccag tataattcca gttatatatt ttcgattacc ttagtcgcta cattaggtgg
     9001 tttattattt ggctacgaca ccgccgttat ttccggtact gttgagtcac tcaataccgt
     9061 ctttgttgct ccacaaaact taagtgaatc cgctgccaac tccctgttag gattttgcgt
     9121 agccagcgct ctgattggtt gcatcatcgg cggtgccctc ggtggttatt gcagtaaccg
     9181 cttcggccgt cgtgattcac ttaagattgc tgctgtcctg ttttttattt ctggtgtagg
     9241 ttctgcctgg ccagaacttg gttttacctc tataaacccg gacaacacag tacctattta
     9301 tctggcaggt tatgtcccgg aatttgttat ttatcgtatt attggcggta ttggcgttgg
     9361 tttagcctca atgctttcac caatgtatat tgcggaactg gctccagctc atattcgcgg
     9421 aaaactggtt tcatttaacc agtttgcgat tattttcggg caacttttag tttactgcgt
     9481 aaactatttt attgcccgtt ccggtgatgc cagctggctg aatactgacg gctggcgtta
     9541 tatgtttgcc tcggaatgta tccctgcact gctgttctta atgctgctgt ataccgtgcc
     9601 agaaagtcct cgctggctga tgtcacgcgg caagcaagaa caggcggaaa gtatcctgcg
     9661 caaaattatg ggcaacacgc ttgcaactca ggcagtacag gaaattaaac actccctgga
     9721 tcatggccgc aaaaccggtg gtcgtctgct gatgtttggc gtgggcgtga ttgtcatcgg
     9781 cgttatgctc tccatcttcc agcaatttgt cggcatcaat gtggtgctgt actacgcgcc
     9841 ggaagtgttc aaaacgctgg gggccagcac ggatatcgcg ctgttgcaga ccattattgt
     9901 cggagttatc aacctcacct tcaccgttct ggcaattatg acggtggata aatttggtcg
     9961 taagccactg caaattatcg gcgcactcgg aatggcaatc ggtatgttta gcctcggtac
    10021 cgcgttttac actcaggcac cgggtattgt ggcgctactg tcgatgctgt tctatgttgc
    10081 tgcctttgcc atgtcctggg gtccggtatg ctgggtaccg ctgtcggaaa tcttcccgaa
    10141 tgctattcgt ggtaaagcgc tggcaatcgc ggtggcggcc cagtggctgg cgaactactt
    10201 cgtctcctgg accttcccga tgatggacaa aaactcctgg ctggtggccc atttccacaa
    10261 cggtttctcc tactggattt acgggtgtat gggcgttctg gcagcactat ttatgtggaa
    10321 atttgtcccg gaaaccaaag gtaaaaccct tgaggagctg gaagccctct gggaaccgga
    10381 aacgaagaaa acacaacaaa ctgctacgct gtaatcttcc tgtccagcac gccgcgccag
    10441 ttcggcgtgc tttcttttta ctcccgcttc agccgtttcg aattacacag ccacagagtg
    10501 atcaccagta acaggatcgc agccgagtag atcaacacat ccagtggcga tttatgatcg
    10561 acgatgatca agcgcacaat ggcggtgatc ccaatataga caaagtaacg tagcgggaag
    10621 tgaaaaccgg actgaaagta cttcacaatc agcgcgataa attcgaaata gagaaagtaa
    10681 accaccagtc cttctaccag ctcatatttg ctggtttgtt ctggcgcgaa cagcacatct
    10741 gccagatgca ccgtttcttt gccgaggaag acaaccaaaa tcaggccgag gcacagcagg
    10801 ccaagattga gtacggtctg caaaatggtg gagataaact ccacgcgcgg acgagagagt
    10861 gacgtcatag cagtacctcc ttcacagtgg cgagcttcct gtataacgca aaaatgtgac
    10921 ggagatctat attttttgtg tatgttttgt tcaacctgga tttggagcgc gcctccacca
    10981 aagccggata tacctacccg taggccggat aagccgcgcc tggcggcgca tccggcaaga
    11041 caaaccgcac ccaagccggt aattcaaaaa tcacccattc aggtgattcc cagccaccag
    11101 caatgcgcga aaaatcgaca atgttcctct ggaacaaatg aaacgtaacc tgataaaggt
    11161 ggtaaaaatg aagccttatt ttgctgcttt gatgttatca gtctctgttc ttcctgctta
    11221 tgctggtccg ctcggtactg ctgataaagc cgacctaccg caaagcaatg tctccagccc
    11281 aatgatggcc cagtcgctca ggcaaccaga cctgcaaccg atatcgacgg acaggaagac
    11341 agaatgtttt cgcctttaca caccagatcg caaaccgggg gtgaactgcg ttcctgatgg
    11401 ctcaacaggc cattaagaat gagggtgcca gtgattgcga aaactggcac ctttcccatg
    11461 acttagcgga aattgacgct acggtcgctg gtcatatcat acaactggaa cttacgccca
    11521 agttgctgac caccgtcacg cgtcagcggc gtccagccaa tcgccgcacg gctgcgtgtc
    11581 gggccagacg agaagagatc caacggtacc gagacataca cgcctttggt gaagtcccct
    11641 tcgccgtact cctcttttga aacattcgtg atcgtggcat agccacccac caccacgccg
    11701 ctatcaaagc gtttggcgat ctccagcgtg ccgcctttat cccctgccag atactgcccg
    11761 acgctggctt tcactaacac gtcctgggcg aaagatggcg tccagtaggc ggtcagatgt
    11821 ccggttttca cgctgtagtc ggtgaatttc atcatatctt ttgcactacg ccagtcgcgc
    11881 tgtttaacgt agttggcatc cagaccaaac gcccagttgc tgtccagcgg acgatacaac
    11941 acttcagccc ccgcaccgcc aaacatggtt tcgagataac ctccatagac ctgaccgtag
    12001 aagccgttgc ccagatgctg gaagtagttg gcttgcaggt tattcacata ggcatcgttc
    12061 tgcacatact cgcgcacatg ggtacgaacg cgcggtaagt gcgagtcctg tggcggattg
    12121 gtgtagttaa acttgtcgta gttgttggca agatttgcaa acaggctgcc gatggtcagc
    12181 agatggtccg tcagccacaa atctgccgtt cccatcacgc ccagctgata catgtaaaag
    12241 ttttccgggc caccgaccga ctggttcagc accggatcga tatggaaatc aaagcgtgat
    12301 ttgtcgatat accagccctg ctcggtggac tgcggaacca ctggctcgac gcgtttttgc
    12361 gccagcgtcg tttcgtggcc caacggctct ccggcgagat gatttttcag gctggcgaca
    12421 tcggtttccg tcgtcacctg cggcatgtta aggcgatttt ccgtaatgcg gatcgttttg
    12481 atcccatccg gcagatcgtt catcacgatc cgattagcac ggatgatccc ttcgcgcgaa
    12541 tcacgatatt tcacctgctc gccggtaaca tacagcgtat cgcctttcgc ctggatctgc
    12601 ggatcggcaa gtccggcatt gtatttcagc aacgttaact gattcgccac caccgagtgc
    12661 tgcaaaatgg catcctgcgg ctgcggttga tattgcgggc gggcgttatc gatgtaggac
    12721 gggcgcagat cgttaaagtt ggtgcgcagc gtgacgccaa acataaaggt gttgccacgt
    12781 tcatagctaa ggttaacgtc ggcccaatcg gtaacgcgat aaatcgcccc gacgttaaac
    12841 ttactttttt gctccagctt cccggcaaaa tcctgctgat aattattacc ttcatactcc
    12901 agtttcaggc gcagcggttg ccagggcgtc tggtattcca cgccgccaaa cagtgaggcc
    12961 gggccgtgga acatctgact gccgtcgata gatcccgctt gtttgtagct gttatcgcga
    13021 tagcaatatt tatcactggc tgaacagagc ggatttttca cattaccgct ggtgcccaga
    13081 tatccccagc ccaggccgag cgtaaaatcg aacggccccc aggctttgct ggcaacaaga
    13141 tattccgcat caaacagccc cgtaccgccg atatcccgcg caccaaccgc cacttgcggc
    13201 agccagtaac tctcttccca caaacgcagt ttgagatcga aggctttatc tttatacgtt
    13261 tgatcgccag agaacgcttc gacgctgctg tactgccggg tgcgcacgtc ggtgtaacgc
    13321 agcgttgttt ccagccacgg gaagagttgc actgaagctg agtaataacg gtactgatcg
    13381 ttatcgcgat agttcagact caactcccct tcccgtgcca tacgcgcggt gggagtttgt
    13441 aataatccta cgccaccgaa atccgactgc gacggaccaa tgggcgcagg atatgtttcg
    13501 ccgtagcaag ctgtgctaat gcccagcgcc agtaagctaa gcagatgtct ttttttcatt
    13561 attggggtat ccgctgcgtc agagtctgaa gaatgtcggc gttaagggca tcaggcgtct
    13621 cactccagac ggagtctgcg aggccaacat aaataatgct gccgggcatc ggctctacgt
    13681 gacgcttgtt ccagtaagcc accggcgctt tttgcgtgcg tccgtccggg taaacgaccc
    13741 acgcgtagct gcgatccgca ccgctgagca ggctctggtc agagagatag ctcgccacgt
    13801 cgcgacctgg agtgaatggc tgattgccag gatggctgat aagcccgaac aacgtgaccg
    13861 tggacggcgg tggtccgacc cacagcgtgt agttgccctg caacggcggg ttaccgcgtt
    13921 cggcaacgcg gacgatatcg ggatcaagat tgattttttg cctgcctgtc acttccaatg
    13981 cctggatttg ctggcgtaag gcgttaatgg cggcggcatc gtcggtgctg gaatctgcgc
    14041 cctgttctgc cagccgtgtc agcaacgcct gctgctgacg caatgctgcc gccgttgcca
    14101 gctcttcgct aatcaccgca ccaggccacc agctgtttgc cagtcgaggt tgccctacca
    14161 gatcgattaa atgctctgcg cccgttagcg ttttggcctc actgctgcca ttgctgaaca
    14221 ccttgacggt tcctgccgcg aagaccgatg acgctcccac actcaaaagc aacgcgacaa
    14281 tagtttgttt aatcattgtt ttgccgcctt gatgagagtg gttttcaccg ggaaataatc
    14341 cgcgccgaga tactgttccg actggcgaat ttgcccttcg ctgtcgatcc agtagcggtt
    14401 atgccagcgc gcctggtcgg tggagacttc ttcgtccagc acgcgaacca gggtttcatc
    14461 gctgccgacc ttgacggtat cggtgccatc ccatttgaag actgagcgtg cggtggcgta
    14521 gcgtacctgc tggtactcgg tccagcccat cgtgcgcgtc catgttgcac catcaacaat
    14581 ttgtgcgggt ttaatcagcg ggtcggcggc aaggttattc acttcaatca ggttgtcgcc
    14641 gccaagcaaa gtcttcacca gacgaccatg ttgtgtgaca agagtggctt gatcctgagt
    14701 gacccatttt tgttgtccgt cttcagcgaa ggccagcacc acaaacaatt gcggcccgcc
    14761 attaagctgc atgtactggc tggcgtaggg catattttga atatcatcgt ccgtcagctg
    14821 tacgcctggc gtgccgaaca gactgtccca cagtgaattg cccagctctt tagtcgtggc
    14881 tgaacaggcc tgtaataaca ggcagataag aatgagtgca ggtcgcttca cgactcttct
    14941 ccagaggggc agaataacca caccggagtg tggttatggt taatcccttc atatttcaag
    15001 ccacagatgc aatgactcga attattttgg ggatacatac tttattactg ggtactggtg
    15061 gttgtggttg ttgtggtccc ggtattagaa ccatcaccgc caccggttgc cgcgagcgcg
    15121 acacccaccg ccgagcttac ggtgctgacg ctggtcgcgg aggaactccc cgccgaaacc
    15181 gacgttgctg ccgcccctgc cgcttcgccc acctgtacag gtgcagccca cacagaagtc
    15241 gccgcaagcg cagatatggc aaaaatgcca tacagaactt ttttcataac aatttccctt
    15301 cattgaatga atggagattt acccaaaaac atttcgggcg ggatcgagta tacacaacta
    15361 aagcatgcga attacggcgc ggggaattag cgatggtgtt ttaggataat tggaatgaat
    15421 atcaaatata agtttcaact tatatttaaa taacaataaa aatcataaac ttaaaataaa
    15481 aacaaaagta attaatctgc gtattctccg aaataaacaa cgattaaaaa tctattttag
    15541 gattaacctc acggtatgat ttccgttaaa tcacagacaa ggggatttat ctgataaaac
    15601 aatgccgact tattgtcggc attgttttta aggttattta actcctcata ctatgcatta
    15661 atgtcgatat tggcatcagg ctccggttgc cggatgcggc gtaaacgcct tatccggcct
    15721 acatatcggc atcaggcacg catgatgatt aaccgcgcca cgctttatag cggttaatca
    15781 gaccattggt cgagctatcg tggctgctga tttctttgtc atctttcagc tctggcagaa
    15841 tacggttcgc cagttgttta cccaactcca cgccccactg gtcgaaggta aagatgttca
    15901 ggatcacgcc ctgagtaaag attttgtgct catacagcgc aatcaacgca cccaggctga
    15961 acggagtgat ttcgcgcagc aggatggagt tggtcgggcg gttaccttcg aacactttga
    16021 acggcaccac gtagtcaagc gttgccggat ctttaccctg atcgcgatat tcctgctcaa
    16081 ccacttcgcg ggatttacca aacgccagcg cttcggtctg ggcgaagaag ttagacagca
    16141 gtttctggtg gtgatcagag agcgggttat gggtgatagc cggggcgatg aaatcgcacg
    16201 gtaccatttt ggttccctgg tggatcagct ggtagaacgc gtgctgaccg ttagtgcctg
    16261 gttcacccca gataatcggg ccagtctggt aatccacaac gttaccgtta cggtcaacat
    16321 acttaccgtt ggactccata ttgccctgct ggaagtacgc cgcgaaacgg tgcatatact
    16381 ggtcatacgg cagaatcgct tcagtttccg caccaaagaa attgttgtac cagatgccaa
    16441 tcagcgccag caggacaggc aggtttttct cggcaggcgt ggtggagaaa tgcttgtcca
    16501 tcgcgtgtgc gccagaaagc agttcaacga agttatcaaa gccgatggag agaacaatcg
    16561 acaggccaat cgctgaccac aaagagtaac ggccgccgac ccagtcccag aactcgaaca
    16621 tgttggcagt atcaatacca aactcgccaa cggctttggc attggtggaa agcgccgcaa
    16681 agtgttttgc aacgtgtttt tcatcacctg ccgctttcag gaaccagtca cgcgcgctat
    16741 gggcgttggt catagtttcc tgagtggtga aggttttaga tgctaccagg aacagcgtgg
    16801 tttccgggtt tacttttttc agcacttcag cgatgtgagt cccatcgacg ttagaaacaa
    16861 agtgcatgtt caggtgattt ttgtacggac gcagtgcttc ggtcaccatg tacgggccga
    16921 ggtcagaacc accgatcccg atgttcacta cgtcagtgat tgctttgccg gtataaccct
    16981 tccactcacc ggagataatc gcttccgaga aggttttcat cttctccagc accgcgttga
    17041 cttccggcat tacgtctttg ccatcaacca gaatcggggt attgctacgg ttacgcagcg
    17101 ctacgtgcag cacggcgcgg ttttcagtgc ggttgatctt ctcgccagag aacatcgact
    17161 taatcgcgcc cgccagatcg cactctttcg ccagatcctg taatttcgcc agcgtctctt
    17221 cagtgatgcg gtttttggag taatccacca gcatctgatc gtcgaatgtt gcggagaact
    17281 tagaaaaacg atcgccgtct ttagcaaaaa gatcggcgat cgtaacgtct ttcatttcat
    17341 cgaagtgttt ctgtagtgcc tgccaggcag cggtctgcgt tggattgatg tttttcatta
    17401 gcaatactct tctgattttg agaattgtga ctttggaaga ttgtagcgcc agtcacagaa
    17461 aaatgtgatg gttttagtgc cgttagcgta atgttgagtg taaaccctta gcgcagtgaa
    17521 gcatttatta gctgaactac tgaccgccag gagtggatga aaaatccgca tgaccccgtc
    17581 gttgacaacc gccccgctca ccctttattt ataaatgtac tacctgcgct agcgcaggcc
    17641 agaagaggcg cgttgcccaa gtaacggtgt tggaggagcc agtcctgtga taacacctga
    17701 gggggtgcat cgccgaggtg attgaacggc tggccacgtt catcatcggc tacaggggct
    17761 gaatcccctg ggttgtcacc agaagcgttc gcagtcgggc gtttcgcaag tggtggagca
    17821 cttctgggtg aaaatagtag cgaagtatcg ctctgcgccc acccgtcttc cgctcttccc
    17881 ttgtgccaag gctgaaaatg gatcccctga cacgaggtag ttatgtctga aattgttgtc
    17941 tccaaatttg gcggtaccag cgtagctgat tttgacgcca tgaaccgcag cgctgatatt
    18001 gtgctttctg atgccaacgt gcgtttagta gtcctctcgg cttctgctgg tatcactaat
    18061 ctgctggtcg ctttagcgga agggctggaa cctggcgagc gattcgaaaa actcgacgct
    18121 attcgcaaca tccagtttgc cattctggaa cgtctgcgtt acccgaacgt tatccgtgaa
    18181 gagattgaac gtctgctgga gaacattact gttctggcag aagcggcggc gctggcaacg
    18241 tctccggcgc tgacagatga gctggtcagc cacggcgagc tgatgtcgac cctgctgttt
    18301 gtcgaaatcc tgcgcgaacg cgatgttcag gcacagtggt ttgatgtacg taaagtgatg
    18361 cgtaccaacg accgatttgg tcgtgcagag ccagatgtag ccgcgctggc ggaactggcc
    18421 gcgctgcagc tgctcccacg cctcaatgaa ggcttagtga tcacccaggg atttatcggt
    18481 agcgaaaata aaggtcgtac aacgacgctt ggccgtggag gcagcgatta tacggcagcc
    18541 ttgctggcgg aggctttaca cgcatctcgt gttgatatct ggaccgacgt cccgggcatc
    18601 tacaccaccg atccacgcgt agtttccgca gcaaaacgca ttgatgaaat cgcgtttgcc
    18661 gaagcggcag agatggcaac ttttggtgca aaagtactgc atccggcaac gttgctaccc
    18721 gcagtacgca gcgatatccc ggtctttgtc ggctccagca aagacccacg cgcaggtggt
    18781 acgctggtgt gcaataaaac tgaaaatccg ccgctgttcc gcgcgctggc gcttcgtcgc
    18841 aatcagactc tgctcacttt gcacagcctg aatatgctgc attctcgcgg tttcctcgcg
    18901 gaagttttcg gcatcctcgc gcggcataat atttcggtag acttaatcac cacgtcagaa
    18961 gtgagcgtgg cattaaccct tgataccacc ggttcaacct ccactggcga tacgttgctg
    19021 acgcaatctc tgctgatgga gctttccgca ctgtgccggg tggaagtaga agaaggtctg
    19081 gcgctggtcg cgttgattgg caatgacctg tcaaaagcct gcggcgttgg caaagaggta
    19141 ttcggcgtac tggaaccgtt caacattcgc atgatttgtt acggcgcatc cagccataac
    19201 ctgtgcttcc tggtgcccgg cgaagatgcc gagcaggtgg tgcaaaaact gcatagtaat
    19261 ttgtttgagt aaatactgta tggcctggaa gctatatttc gggccgtatt gattttcttg
    19321 tcactatgct tatcaataaa cgagcctgta ctctgttaac caacgtcttt atcgagaata
    19381 attgccttta atttttttat ctgcatctct aattaattat cgaaagagat aaatagttaa
    19441 gagaaggcaa aatgaatatt atcagttctg ctcgcaaacg aattcccgta cactgaactc
    19501 tctggctcac tgagggcacc atcatggcac tcccccgtat tacccaaaaa gagatgactg
    19561 aacgcgagca gcgtgaactt aaaacactgc tggatcgggc gcggattgcg catggtcgcg
    19621 tactgaccaa ctccgaaacc aacagcatta agaaagagta catcgataaa ttaatggtcg
    19681 agcgtgaggc tgaagcgaaa aaagcccgcc aattgaagaa aaagcaggct tataaaccgg
    19741 atcctgaagc gtcatttagc tggtcagcaa atacgtcgac gcgcggcagg cgctaatgaa
    19801 caatcaacgc ctgagatttt cactcgtcag tgtgaacaca attttcccct caccctaacc
    19861 cttttcccga aggggcgagg ggactgtccg agcacttttg ggccgttttc atcagtctga
    19921 ggcgttaatc agcgcccctt ctttttacgc cccggcgagg taaaacgctt accgttggac
    19981 gccgggcgac cgcctttttc cgccgttttt tccatcttaa cgactggacg tttgatgccc
    20041 gctgttttcg gtttggcctt cgctttgggc ttcgcctcgg aagaggaatt ttcaatgagc
    20101 ttaaagagat cgataagctc gtcgtcggtt aaatcgcgcc attctcccag cggaatgccg
    20161 cttaagctga cgttcataat gcgcgtacgt tccagctttt tcacttcata gccgaaatgc
    20221 tcgcacatgc gccggatctg acggttcaac ccctgcacca gggtaatgcg gaagacaaac
    20281 ggtgcttctt ttttgacttt gcactttttc gtcaccgtcc cgaggatcgg cactcccgcc
    20341 cccataccac gaataaattc atcggtaatc ggtttatcga ccgtcaccag atactctttc
    20401 tcatgatcgt tacccgcacg gaggatctta ttcaccagat cgccgtgatt ggtgaggaaa
    20461 atcagcccct gggagtcttt atccaggcgg ccaatcggga acacgcgttt gctgtggtta
    20521 acgaaatcga caatgttatc gcgctcgcca tcttcggtgg tgctgacaat accgacgggc
    20581 ttgttcaggg cgataagtac caaatcttcg gcttcccgag gctcaatcaa ctgaccgttt
    20641 actttcacaa tgtcgccggg tttcacctga tcaccaatgg tggctcgctt gccattaagg
    20701 aacacattgc cttgctcgat atagcgatcc gcttcgcggc gtgagcaaat tccgctttcg
    20761 ctgatgtatt tatttaaacg gactgatgag tcgggcagca taaattctcc tgtaaaacgc
    20821 ggaatatacc gtacctctta gcggggaaaa acagggttat gcggcaacgt cgaaattaaa
    20881 tgtgatctgc cacgcctttt atcacaaaag tgtctccgga agtacgatgc aaatttgcct
    20941 ggcaaaataa ggataatcaa catattacga atcgctgcac acctgtgctg atgactacta
    21001 cgcagagaaa caaatgtgca agagaaagcg ctatttctcc ccatgcaaag agtgaaaaat
    21061 cgctttgaaa ggccctcata atggggttat atcagatcaa gcggtgagaa agcgtatttg
    21121 cacaaatgtg cagaaccagg aggcggcaat ggaaaacagt gacgatatcc gtttgattgt
    21181 gaagattgcc caactctatt acgaacagga tatgacgcag gcgcaaatcg cgcgcgaact
    21241 gggtatttac cgcaccacca tcagccgctt gcttaaacga ggccgcgatc agggaattgt
    21301 caccatcgcc atcaactatg actacaacga aaatctctgg ctggagcagc aactgaagca
    21361 aaagtttggc ttgaaagacg ttgtggtggt gtcgggaaat gatgaggatg aagagactca
    21421 actggcgatg atggggttac acggcgcgca actgctggat cgcttgctgg agcctggcga
    21481 tattgtcggt ttttcctagg gtcgcgcggt gagcgcactg gttgaaaact tgccgcaagc
    21541 ggggcaatcg cggcagttaa tctgcgtgcc aattattggc ggaccgtccg gtaaactcga
    21601 aagccgctat cacgtaaaca cattaaccta cagcgtggca gcgaagctga aaggggaatc
    21661 gcatctcgcg gattttccgg ctctgctgga taacccctta attcgtaatg ggatcatgca
    21721 gtctcagcac tttaaaacca tctctgccta ctgggataat ctggatgtcg ccctggtggg
    21781 aattggctca ccggccattc gcgacggcgc taactggcat gcgttttatg gtggtgaaga
    21841 gagtgacgac ctgaaagcag gcgcggtatt ccattggcat tgccatgagt gaagaaaaat
    21901 acagcggaat tgttggtgca ctgcgtggaa aatatattaa ttgtctggta acgaatagca
    21961 gcacagctga actattactg aaataaaaat cttcccgatg tttaccgtaa taaaaattac
    22021 ggcgggtccc tattatctaa aatcaggagt ctgttatgca aacgtggtta aatttgcagg
    22081 ataaaatcat tatcgtcacc ggcggcgcat ccggtattgg tctggcgatt gtagaggaat
    22141 tattagcaca aggcgcaaac gtacagatgg tagatattca cggtggcgat ggtcaatatg
    22201 aagttcataa aggctatcag ttttggccga ccgatatttc cagcgccaaa gaggtaaatc
    22261 atacggtagc ggaaattatc cagcgttttg gtcgcatcga cggtctggtc aataacgccg
    22321 gggtcaattt cccgcgtctg ctggtcgatg agaaagcgcc tgccgggcag tatgaactca
    22381 acgaagctgc attcgaaaaa atggtcaata tcaaccaaaa aggcgttttt ctgatgtcgc
    22441 aggcggtggc gcgccagatg gtcaaacaac atgatggcgt gattgtgaat gtttcctcag
    22501 aaagtggact ggaaggctca gaaggccaaa gctgttacgc cgctaccaaa gccgcgctca
    22561 atagcttcac gcgctcctgg tcgaaagagc tgggtaaaca cggtatccgt gtggtcggta
    22621 tcgcgccggg gattctggaa aaaaccgggc tacgcacgcc ggaatatgaa gaggcgctgg
    22681 cgtggacgcg caatatcacc gtcgagcagc tgcgtgaagg ctataccaaa aacgccattc
    22741 ctattgggcg cgccggaaga ttagcagaag tcgctgattt tgtttgttat ctgctgtctg
    22801 aacgcgccag ctacatcacc ggagtaacca ctaacattgc gggcggcaaa acgcgcggat
    22861 aaggaggctt tatggtcaac gccatttttt gtgcccacgg caaattagct tgcgccatgc
    22921 tggaatcggt tcagatggtg tacggcgacg ccaatgtcga ggcggttgaa tttgtaccag
    22981 gcgagaacgc cggagacatc gtagcaaagc tggaaaagtt agtaagcatt cacaatcaag
    23041 atgagtggct gattgccgtg gatttacagt gcggcagccc gtggaatgcg gcagcgatgc
    23101 ttgcgatgcg caatcctcgt ctacgggtaa tcagcggcct ttccctgccg ctggcgctcg
    23161 aactggttga taaccaggac agcatgaatg ttgatgaact gtgtgaacac ctgacgcaaa
    23221 tcgcaaagca aacctgcgtt gtctggaaac aactggcaac ggcagaggaa gatttctgat
    23281 gaacattacg ctcgcccgca ttgatgaccg tctgatccac ggccaggtca ctaccgtctg
    23341 gtcgaaagtg gctaacgccc agcgcattat tatctgcaat gacgaagttt ataacgatga
    23401 agtgcgccgg acattattgc gtcaggctgc gccaccggga atgaaggtta acgtggtcaa
    23461 tattgaaaaa gccgtcgcgg tttatcacaa cccgcaatat taggacgaaa ccgtttttta
    23521 tcttttcact cgcccacagg atgccttagc gatggttcgc cagggggtaa aaataggcac
    23581 cctgaatatt ggcggcatgg cctggcggcc cggtaaaaaa cagttaacca aagcggtttc
    23641 attagatgat gatgatatta acgcatttca tgagttaaat aatcttggcg tcataatgga
    23701 tttacgcgtc gtagcgtcag atccctcaat caatattatc gacaaaataa acgaacagtt
    23761 aatcgcaaat taaataatag cgcctgtgta catatgcttt aacaggcagg gatgacgaca
    23821 cttaaaaggt gacaaatcat ggaaataagt accctacaaa ttattgctat atttcttttt
    23881 tcctgtattg ccggaatggg cagcgtgctg gatgaatttc agactcatcg cccgttaatt
    23941 gcctgtacgg tgattggttt aattctcggt gatttaaaaa ccggaataat actcggtggt
    24001 acgctggaat tgatagctct cggctggatg aacgtcggcg cggcgcaatc tccggattct
    24061 gcactcgcca gcataatctc cgccattctg gttatcgttg gtcagcaaag catcgccacc
    24121 ggaatcgcca tcgcgttgcc tgtggctgcg gcaggccaag tgctgacggt gtttgcccgt
    24181 accatcaccg tagtgttcca gcacgcggcg gataaagcag cggaagaggc gcggtttcgc
    24241 actctcgata ttctgcatgt ctccgcgctt ggcgtgcagg cgctgcgcgt tgctattccg
    24301 gcactgattg tctcactgtt cgtcagcgcc gatatggtca gcaatatgtt aaacgccatt
    24361 cccgaatttg tgacccgtgg actgcaaatt gctggcggtt ttatcgtggt ggtcggttac
    24421 gccatggtgc tacgtatgat gggcgtgaaa tatttgatgc ctttcttttt cctcggtttc
    24481 ctcgcaggtg gctacctcga tctcagcctg ctggcgttcg gtggcgtcgg cgtgatcatg
    24541 gccctggtct acatccagct aaatccacag tggcgtaaag ctgaacctca actccaggcc
    24601 accgcctcca ccgcccttga ccaacttgac gattaacgga gccatcatgg aacagagaaa
    24661 aattacacgc agcgatctgg tgagcatgtt tctgcgctcc aacctgcaac aggcgtcctt
    24721 taactttgaa cgtattcacg gactgggctt ttgctacgac atgatccccg ccatcaagcg
    24781 actttaccca ttaaaagagg atcaggttgc ggcgctcagg cgacacctgg tgttcttcaa
    24841 taccacgcca gccgtatgtg gcccggtcat cggcgtcact gccgccatgg aagaggcgcg
    24901 ggccaacggc gcggaaattg atgacggtac cattaacggc atcaaagtcg gtctgatggg
    24961 accattggca ggagttggcg atccactggt ctggggaacg ctgcgcccga ttaccgctgc
    25021 gctcggcgca tctctggcac tttctggcaa catcctcggc ccgctgctgt ttttctttat
    25081 tttcaatgcg gtgcgtctgg cgatgaagtg gtatggccta cagctcggct ttcgcaaagg
    25141 ggtgaatatc gtcagcgata tgggcgggaa tgtgctgcaa aaactcaccg aaggcgcgtc
    25201 gattctcgga ctgtttgtga tgggcgtgct ggtcaccaaa tggacgtcaa tcaacgtacc
    25261 gttggtggtt tcacaaacgc atgccgccga tggctccacc gtcatcatga ccgtgcagaa
    25321 cattctcgac caactttgcc ctggtttgct ggcgctcggt ctgacgctac tgatggttcg
    25381 tctgctcaac aaaaaaatta acccggtatg gctgattttc gccctgtttg gcttagggat
    25441 tatcggcaat gcgctgggct tcctgtcctg attcttcgcc ccggcacgac tgccggggcc
    25501 atcgctcaac atgaggtggt ttatgaaaac aacagctctg cgtctttatg gtaaacgtga
    25561 tttacgcctg gaaacctttg accttcctga aatgcaggag gatgaaatcc tcgcgacggt
    25621 ggtcactgac agcctgtgcc tctcttcctg gaaagaggca acctgggtga aaaccataaa
    25681 aaagtacccg acgatgtggc gaccaaccca atcatcatcg gccacgagtt ttgcggcgat
    25741 attctggccg tgggtaaaaa gtggcagcac aaattccagc cgggtcagcg ttatgtgatt
    25801 caggccaacc tgaaactccc tgaccgcccg gactgccccg gctactcctt cccgtgggtg
    25861 ggcggcgaag ccacgcatgt ggttattccc aacgaggtca tggaacaaga ttgcctgctg
    25921 gcatacgacg gcgaaaccta ttttgaaggc tcgctggttg aaccgctttc ctgcgtgatt
    25981 ggcgcgttca acgccaacta tcatcttcag gaaggtagtt ataaccacac gatggggatt
    26041 cgcccgcaag ggcgcacgct gatcctcggc ggcaccggac caatgggatt gttggcgatt
    26101 gattatgcgc tgcatggacc agttaacccg tcgctgctcg tcattaccga taccgacaac
    26161 gacaaattga gttatgcgcg caagcactat ccatcagaac cgcaaacact gattcattat
    26221 ctcaatgcca ccgatgcagc atttgatacg ttaatggcgc tgagtggcgg tcacggcttc
    26281 gatgatattt tcgtctttgt gcctaatgaa ggactggtga ctctcgcctc ttccttgctg
    26341 gcgacagatg gttgcctgaa tttcttcgcc ggaccgcagg ataaacattt cagcgcgcca
    26401 attaatttct acgatgtgca ttatgcattt acccactacg tgggcacgtc aggcggcaat
    26461 accgacgaca tgcgcgcggc ggtcaaactg attgaagaga aaaaagtgca ggccgcaaaa
    26521 gtggtaacac atattcttgg gctgaatgcc gcgggcgaaa ccacgcttga attgcctgcc
    26581 gtcggcggcg gcaaaaagct ggtgtatacc gggaaatacc tgccgctgac gtcactcacg
    26641 cagattcagg atcaagcact ggcggcgatt ctggcgcgtc atcaggggat ctggtccggt
    26701 gaggcggagc aatatctgct cactcatgca gaggcaattt cccatgatta atcgcgctac
    26761 ttaactctgc atgtcgctgg ctggtcgccg cggcaacttc gcgacctgat gtgcggctga
    26821 ttgctgaagc ggcccaatgt gcacgagaag gataaaaaaa catcagcgcc ttaaggggcg
    26881 ctcaaattgg tggcaaagta cacattgttt atgccggatg cggaataaag ccttattcgg
    26941 cctacataat ctcgaaaatt caatatattg cagttatctg gtaggcctgg taagcgtagc
    27001 gcatcaggca atgctgtgtt tgtcatcagt ctcagcgccc ggtgcaatac gtgacatcgt
    27061 gagccatgtc agtaagtggt taaattcgca gtggtagcat gccttccgtg atttcttact
    27121 aaccggagaa gtaatggaac tgcttttatt gagtaactcg acgctgccgg gtaaagcctg
    27181 gctggaacat gcactgccgc taattgctga acagttgcag ggtcgccgct cagcggtgtt
    27241 tatccctttc gctggcgtaa cgcagacctg tgatgattac acagcgaaaa cggctgcggt
    27301 tctcgctccg ctgggtgttt ctgtcaccgg tattcatagc gttgtcgatc ccgttgccgc
    27361 gattgaaaat gctgagatcg tgattgtcgg ggggcgggaa tactttccag ttgctgaaac
    27421 agtgccgcga gcgcgggctg ctggcaccaa ttactgacgt ggttaaacgt ggtgcgctgt
    27481 atattggctg gagcgcaggc gctaaccttg cttgcccaac cattcgtacc accaacgata
    27541 tgccgattgt cgatccgcaa ggtttcgatg cgctaaatct gttcccgctg caaatcaacc
    27601 cgcacttcac caacgcgctg ccggaaggcc ataaaggtga aacccgtgag cagcgtattc
    27661 gcgaactgct ggtcgtcgcg ccagaactga cgattattgg tctaccggaa ggtaactgga
    27721 tcacggtgag taaaggtcac gctacgctgg gtggcccgaa caccacttat gtgtttaagg
    27781 ccggtgaaga agcggtgccg ctggaagcgg gtcaccgttt ttaagtgaat tgccggatgc
    27841 atgatccggc atcttgttca tagtgcctga tgcgacgctt gacgcgtctt atcaggccta
    27901 caggattcac aatattaata ctcgtcccgt ccttcatctt catccggctg ttccagcaca
    27961 ctgtaagcca ccgaacaaaa cagcgagttc agacgctgca tatctcccag taagcctaaa
    28021 tgtagcgaac tggtttcaat gctctgcacg ttttgctgat gcaggcgatc gacgtgggcg
    28081 tgggaatagc ggcgattaag aatgcgaaag cgatgcttgc tgcggcgcaa acgacgagcg
    28141 ctggtgacat cgccagagaa gaacaccgac attgccagct ttaaattact gagcaattgc
    28201 tcatagagcg catccagttc cttcaaccca tcaagcgaaa atgcccgccg tgctgccagc
    28261 gatttatcag caatttcgct gcccatgcgc tcgacgatat cggaagcctg ttcaaggttg
    28321 agcgacattt caataatctc cgcccagcga cgcgactctt cttctgccag ttcctctttt
    28381 ggcattcgcg ccagatacag cttaatggcg gtgtagagaa cgttgatatc atctgccagc
    28441 ttacgcagct ctttctcctg ccgtggctcg ccgtgcatca ctttattcag cccttccatc
    28501 atctgttcca tggcgtcgcc aatgcgcagg gtttcgcgcg cggcgttcgc cagagcaagc
    28561 gtgggcgtat ccagcgcgct gacatccaga tgtttaggcc gtagctgggt atccagttcc
    28621 ggttcatcgc gaataatcgt tttgcaaaac cgtgccatcg ggtcaacaaa tggcagcatg
    28681 accaggcaac gtacaaggtt gtagaagacg tggaaataga tcaccagttc cgcttttggc
    28741 aatgacaact tccccattgt ctctgccagc aaatggacaa acggcaggat aatcaggcta
    28801 cccaccagct taaacagcaa actacccagc gcgacacggc gggctgcggc attggcggca
    28861 ctgttgttga gcatcgccag caggccggaa ccgaggttag caccaatcac cagacagagc
    28921 gccacgggga aggagataat gcctgcggcg gtcagagtcg cggtcagcag tacagcagca
    28981 aggctggagt agctgataat ggcgaacatc gcgccaatca gcgcatccag cagaatatcg
    29041 ccggtcagcg aggcaaagat cacctgaacg ccgtttgcct gggtgatcgg cgttacggcc
    29101 tgcacaatca actccagcgc cagcaaaatc agcccaagac caataccgac gcggcccagt
    29161 tgcccggcgc gtgactgttt gcgtccgagg aaaaaaatca cgccgataaa aataagtaac
    29221 ggtgacagcc aggataagtc gaaggtgaga atacgcgcca ttagcgccgt cccgacatct
    29281 gcacccagca caatcaccag agccggtgcg agggctacca gatcctgagc gacgaacgag
    29341 gtcaccagca tggtggtggc attactgctc tgtaccagtg cggtaacgcc gatccccgcg
    29401 caaaaggcga gcggcttttt ttcgacgctc cggctaagga cagtacgcaa acgcgcgccg
    29461 aagacgcgca ttacgccggt tcgaacaata tgagtccccc agaccagcag ggcgacggca
    29521 gaaagcaggt gaagcagcgt taacacgagt tcaggttctc cttatcgtta tatgttcctg
    29581 aagcaattga atgaccaaag gaccggaaac acgctgactg gcatctttac tgtgcctttt
    29641 gacattcgtt tttccggcta agattattcc agcctgtttc cagtataagt gtttcatatt
    29701 aataaagaga cagggcactc attgagcgcc ctgtttgttg taaggaaaag tgacagattt
    29761 gtgaatcagt ccgcgtcata ccccagattc ggtgccagcc agcgctcaac atcggagacg
    29821 ctcatacctt tacggcgggc atagtcttca acctgatcgc gctgaatttg cgccaccgcg
    29881 tagtacttgc tgtccgggtg gctgaaatac caacccgaaa ccgatgcacc aggccacatg
    29941 gcgaaagatt ctgtgagttt catgccagta tgtttttcca cttccagcag ctcccagatg
    30001 gtggcttttt ccgtatgttc cgggcaggcc gggtagcccg gtgccggacg gatgccctgg
    30061 tagttttcgc ggatcagttc ttcgttgctg aggttctcgt tcggcgcata gccccagtag
    30121 actttacgca cacgctcatg gagatactcc gcaaaggctt cggctaaacg gtcggcaagc
    30181 gctttcacca tgattttgtt gtaatcatcg tgctgcgctt caaaggcatc agccagtgcg
    30241 tcctcttcca gcccgccagt cacggcaaat gcgccgatgt aatctgcttt accagaaagc
    30301 ttcggcgcaa cgaagtcagc gagacagtag ttagcgaatc ccgttttttc ggtctgttga
    30361 cgcagatggt ggctgacgtt gatcacatgg gtgcgcgttt cgtcacggta gatttcaatg
    30421 tcatcgccca cacggtttgc cgggaacaag cccaccacgc cacgcggatt cagcgttttc
    30481 tcggcgctta atttatccag catgtcgttg gcgtctttaa acagccgctg cgcctcaacg
    30541 cccaccactt catcttccag aatgcgcgga tacttcccgg ccagcgacca ggtcataaag
    30601 aacggtgtcc agtcgatgta attacgcaga gtttcgatgc tggcttcaac ttcctgcacg
    30661 ccgagacggt gcgccaccgg cggcgtgtaa gcctgccagt caaaagcgaa gtcgttatcg
    30721 cgcgccgctt ccagcgtgac cggtggtgtg cgcggtttct tgcgcccgtg ctgaatacgt
    30781 acggtttcat actccttgcg ggtacgagcg acaaaatcat cgcgctgagt atcggaaagc
    30841 agcgccgcca ccacaccaac ggtgcgcgag gcattctgca catacaccgt cgggccgctg
    30901 tagttctgct caattttcac cgccgtgtgc gcttttgagg tcgtcgcgcc accgatcagt
    30961 aacggaatgg tgaagccctg acgctccatc tctttcgcca cgttaaccat ctcgtccagc
    31021 gacggcgtga taagccccga aagtccaatc agatcagcat tcacttcttt agcggtacgg
    31081 agaatttttt ccgcaggcac cataacgccg agatcgacaa tttcgtagtt gttacattgc
    31141 agcaccacac caacgatatt tttaccgatg tcgtggacgt cgcccttcac ggtggcgatc
    31201 accatcttgc cgttggtttt gccctgctct ttgctggctt caataaacgg ttcgaggtag
    31261 gccactgcct gtttcatgac gcgcgccgat ttgaccacct gtggcaggaa cattttcccc
    31321 tcgccaaaca gatcgccgac cacgttcatg ccgtccatca acgggccttc aatcacttca
    31381 atcgggcgcg tagcctgctg gcgggcttct tcggtatcct gctcgataaa ctcagtaatg
    31441 cctttgacca gcgagtattc cagacgttta ttcacttccc acgagcgcca ctccgcctgc
    31501 tgggcgttag cggtgtcgtc ggttttgctg ccgcgatatt tctcggcaag ctccagtaaa
    31561 cgttcagtgc catcatcgcg acgattaaga atcacatctt ccaccgcgtc gcgcagttca
    31621 gcgggcaggt cgtcgtaaat cgccagttgc ccggcgttga cgatccccat atccatgcca
    31681 ttgcgaatag cgtagtagag gaacaccgcg tgaatggctt cgcgcaccgg ttcgttgcca
    31741 cggaatgaga aagaaacgtt agatacgccg ccggaaatca gcgcgtgcgg cagttcgcgt
    31801 ttgatgtctt cgcaggcgcc gataaagtcc tgcgcatagt tgttgtgctc ttcaatgccg
    31861 gtggcgaccg cgaagatgtt cgggtcgaag atgatatctt ctggcgggaa gccaacctct
    31921 tcggtgagga ttttgtacgc ccgacggcaa atctcgattt tccgtgcgcg ggtatcggcc
    31981 tgtccctgtt cgtcaaaggc cattaccacc accgctgcac cgtaacgacg caacagtttc
    32041 gcatgatgga taaaggcgtc gacgccctct ttcattgaga tagagttaac aatgcctttg
    32101 ccctgaatac acttcagccc cttttcgatg acctcccatt ttgaggagtc gatcataatc
    32161 ggcacgcgag cgatatccgg ttcaccggca atcagattga gaaaacgcac catcgccgct
    32221 tcggcgtcga gcatcccttc atccatattg atatcgataa tctgcgcgcc gttttccacc
    32281 tgctggcggg ccacatccag tgcctcgctg tatttctctt ctttgatcag gcgcttgaac
    32341 ttagcggaac cggtgacgtt ggtgcgttca cccacgttca caaacaggct atcttcgcca
    32401 atgttcagcg gctccaggcc ggacaaacgg caggctacgg ggatttccgg cagtttgcgc
    32461 ggcgctaatc cttctactgc acgactcatc gctgcaatat gttgtggcgt ggtgccacag
    32521 cagccgccga cgatattgag aaagcccgct tccgcccatt cacgtatttg ttttgccatc
    32581 gtgtcggcgt cgagatcgta ctcaccaaag gcgttgggta gcccggcgtt cgggtgcgcg
    32641 gtgacgtagc attccgcaat ccgtgacagc tcctgcacgt actggcgcag ttcatcgggc
    32701 cccagcgcac agttcaggcc aaaggtcagc gcttcggcgt ggcgcaatga gttgtaaaat
    32761 gcttcggtgg tctgcccgga gagcgtgcgc ccggaggcgt cggtgatggt gccagagatc
    32821 ataatcggca gctcaacgcc cagcgtttca aactccgttt ttaccgcaaa taccgccgct
    32881 ttggcgttaa gggtgtcgaa aacggtttca atcaggatca gatccgcacc accttccacc
    32941 agcgctttgg tggactctcg ataagccacc accagcccgt caaaagtgat attacgaaat
    33001 gccggatcgt tgacgtccgg agaaatagac gccgtgcggt tggttgggcc gagaacaccg
    33061 gcaacgtagc gcggtttctc cggcgtgcgc gcggtccact cgtcagcaca ggcgcgcgcc
    33121 agtttcgccg ccgcaaagtt gatttccgcc gacagggatt ccatctggta atccgccatc
    33181 gcaatggtcg tggagttgaa ggtgttggtt tcgatgatat ccgcgcccgc ttcaaagtag
    33241 gcgttgtgga tagcggcgat cacttccggt ttactgagta ccagcaggtc gttgttgcct
    33301 ttgaggtcgc atggccagtc ggcaaagcgt tcaccacgaa aatcggcttc gttcagtcga
    33361 tagctctgga tcatggtgcc cataccgccg tccagcacca gaatacgttc atttaactgc
    33421 gcacgcagtt gttccacttt gctgctcaca cttgctcccg acacgctcaa cccagattta
    33481 ataaaaattc aacaaaccat actggcataa acgcatctgt ggtaaaagcg accaccacgc
    33541 aacatgagat ttgttcaaca ttaactcatc ggatcagttc agcaactatt gcattaacta
    33601 acaataaaaa tgaaaatgat ttccacgata cagaaaaagg agactgtcat ggtcgcaccc
    33661 attcccgcga aacgcggcag aaaacccgcc gttgccaccg caccagcgac tggacaggtt
    33721 cagtctttaa cgcgtggcct gaaattactg gagtggattg ccgaatccaa tggcagtgtg
    33781 gcactcacgg aactggcgca acaagccggg ttacccaatt ccacgaccca ccgcctgcta
    33841 accacgatgc aacagcaggg tttcgtgcgt caggttggcg aactgggaca ctgggcaatc
    33901 ggcgcacatg cctttatggt cggcagcagc tttctccaga gccgtaattt gttagcgatt
    33961 gttcacccta tcctgcgcaa tctaatggaa gagtctggcg aaacggtcaa tatggcggtg
    34021 cttgatcaaa gcgatcacga agcgattatt atcgatcagg tacagtgtac tcacctgatg
    34081 cgtatgtccg cacctatcgg cggtaaattg ccgatgcacg cttccggtgc aggtaaagcc
    34141 tttttagccc aactgagcga agaacaggtg acgaagctgc tgcaccgcaa agggttacat
    34201 gcctataccc acgcaacgct ggtgtctcct gtgcatttaa aagaagatct cgcccaaacg
    34261 cgcaaacggg gctattcatt tgacgatgag gaacatgcac tggggctacg ttgccttgca
    34321 gcgtgtattt tcgatgagca ccgtgaaccg tttgccgcaa tttctatttc cggaccgatt
    34381 tcacgtatta ccgatgaccg cgtgaccgag tttggcgcga tggtgattaa agcggcgaag
    34441 gaagtgacgc tggcgtacgg tggaatgcgc tgactttttc tggcgggcag aggcaatatt
    34501 ctgcccatca tacctgagtg gcaatagaat aagggtgtct gttaatcgca ttgacgccaa
    34561 aataacttaa tgtcatacac ttcacacact tttattaact cagcattatt tttaaacatc
    34621 aaaccactta attattaaca aaatcgcaag atctcattcc accgaaactt ttacggctgc
    34681 attatgagat tgagcgattt atgatatttc tttagttgta atcatattaa attttttcac
    34741 tgttctgata gttaaaattt aagacatcaa taaacaatga gatatttaaa tgattactcg
    34801 tattcctcgt agttctttct ctgcaaatat taataataca gcccagacaa atgaacacca
    34861 aaccctgagt gaattgtttt ataaagaact cgaggataaa ttttctggca aggagctggc
    34921 gacgcctcta ttaaaaagct tctcagagaa ctgtagacaa aatggtcgtc atatctttag
    34981 caacaaggat tttgtcatta aattttccac gtccgtctta caagctgata agaaagaaat
    35041 tacgataatt aataaaacga aaacacgaca ctcactcaaa ccattgctcc aatatttgaa
    35101 gaatacctaa tggaaatttt acctcaacgc tcagacactc ttgataaaca agaattaaac
    35161 ctaaaatcag atagaaaaga aaaagaattc ccaagaatta aacttaatgg tcaatgctat
    35221 tttccggggc gaccccaaaa ccgtatagta tgccgacaca ttgctgcaca atatattaat
    35281 gatatttatc agaatgttga ttacaaaccc catcaagatg attattcttc agctgaaaaa
    35341 tttctcactc acttcaacaa aaaatgcaaa aaccagactt tggcgttgat ttccagccgt
    35401 cctgaggggc gttgcgttgc tgcctgcggt gatttcgggc tagttatgaa agcatatttt
    35461 gacaagatgg aatcaaatgg catcagtgtt atggcagcca tattactggt ggataaccat
    35521 gctttgacgg tccggctaag aataaagaac acaactgaag gatgtaccca ttacgtggtt
    35581 tcggtttatg atcctaatgt aactaacgac aaaataagaa tcatgagcga aagcaaagag
    35641 gatattaaac actattctct gatggatttt atgaatgtag attatagcct cctgaaatgg
    35701 tcaaatgatc atgttattaa ccaatctgtt gcaataattc cagcacttcc gaaagaacag
    35761 ctattgatat taaaaggat
//
LOCUS       DAJVQG010000024        35766 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJVQG010000024 DAJVQG010000000
VERSION     DAJVQG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35766
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.024"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            417..1865
                     /gene="uacT"
                     /locus_tag="OQM83_001088"
     CDS             417..1865
                     /gene="uacT"
                     /locus_tag="OQM83_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCS1519399.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPFIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFLHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            1867..1992
                     /locus_tag="OQM83_001089"
     CDS             1867..1992
                     /locus_tag="OQM83_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1519400.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(1989..2060)
                     /locus_tag="OQM83_001090"
     CDS             complement(1989..2060)
                     /locus_tag="OQM83_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1519401.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            2115..2663
                     /gene="idi"
                     /locus_tag="OQM83_001091"
     CDS             2115..2663
                     /gene="idi"
                     /locus_tag="OQM83_001091"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCS1519402.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="OQM83_001092"
     CDS             complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="OQM83_001092"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCS1519403.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(4233..5331)
                     /gene="prfB"
                     /locus_tag="OQM83_001093"
     CDS             complement(join(4233..5255,5257..5331))
                     /gene="prfB"
                     /locus_tag="OQM83_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCS1519404.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="OQM83_001094"
     CDS             complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="OQM83_001094"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCS1519405.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="OQM83_001095"
     CDS             complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="OQM83_001095"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCS1519406.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="OQM83_001096"
     CDS             complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="OQM83_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCS1519407.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            8904..9425
                     /gene="fldB"
                     /locus_tag="OQM83_001097"
     CDS             8904..9425
                     /gene="fldB"
                     /locus_tag="OQM83_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCS1519408.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(9465..9872)
                     /locus_tag="OQM83_001098"
     CDS             complement(9465..9872)
                     /locus_tag="OQM83_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HCS1519409.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="OQM83_001099"
     CDS             complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="OQM83_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCS1519410.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10362..11342
                     /gene="ygfZ"
                     /locus_tag="OQM83_001100"
     CDS             10362..11342
                     /gene="ygfZ"
                     /locus_tag="OQM83_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCS1519411.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(11538..12197)
                     /locus_tag="OQM83_001101"
     CDS             complement(11538..12197)
                     /locus_tag="OQM83_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCS1519412.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(12361..12672)
                     /locus_tag="OQM83_001102"
     CDS             complement(12361..12672)
                     /locus_tag="OQM83_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCS1519413.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            12717..14150
                     /gene="bglA"
                     /locus_tag="OQM83_001103"
     CDS             12717..14150
                     /gene="bglA"
                     /locus_tag="OQM83_001103"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCS1519414.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            complement(14207..14950)
                     /locus_tag="OQM83_001104"
     CDS             complement(14207..14950)
                     /locus_tag="OQM83_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1519415.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVFSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="OQM83_001105"
     CDS             complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="OQM83_001105"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCS1519416.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="OQM83_001106"
     CDS             complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="OQM83_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCS1519417.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="OQM83_001107"
     CDS             complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="OQM83_001107"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCS1519418.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            20005..20088
                     /locus_tag="OQM83_001108"
     CDS             20005..20088
                     /locus_tag="OQM83_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1519419.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="OQM83_001109"
                     /gene_synonym="visC"
     CDS             complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="OQM83_001109"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCS1519420.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="OQM83_001110"
                     /gene_synonym="visB"
     CDS             complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="OQM83_001110"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCS1519421.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="OQM83_001111"
     CDS             complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="OQM83_001111"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCS1519422.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNELEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="OQM83_001112"
     CDS             complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="OQM83_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCS1519423.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24659..24988
                     /gene="zapA"
                     /locus_tag="OQM83_001113"
     CDS             24659..24988
                     /gene="zapA"
                     /locus_tag="OQM83_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCS1519424.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25030..25213
                     /gene="ssrS"
                     /locus_tag="OQM83_001114"
     ncRNA           25030..25213
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OQM83_001114"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            25234..25836
                     /locus_tag="OQM83_001115"
     CDS             25234..25836
                     /locus_tag="OQM83_001115"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCS1519425.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(25937..25996)
                     /locus_tag="OQM83_001116"
     CDS             complement(25937..25996)
                     /locus_tag="OQM83_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1519426.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="OQM83_001117"
     CDS             complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="OQM83_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCS1519427.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(26616..27848)
                     /gene="serA"
                     /locus_tag="OQM83_001118"
     CDS             complement(26616..27848)
                     /gene="serA"
                     /locus_tag="OQM83_001118"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCS1519428.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="OQM83_001119"
     CDS             complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="OQM83_001119"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCS1519429.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            29193..30086
                     /gene="argP"
                     /locus_tag="OQM83_001120"
     CDS             29193..30086
                     /gene="argP"
                     /locus_tag="OQM83_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCS1519430.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            30289..32433
                     /gene="scpA"
                     /locus_tag="OQM83_001121"
     CDS             30289..32433
                     /gene="scpA"
                     /locus_tag="OQM83_001121"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCS1519431.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            32426..33421
                     /gene="meaB"
                     /locus_tag="OQM83_001122"
     CDS             32426..33421
                     /gene="meaB"
                     /locus_tag="OQM83_001122"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCS1519432.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            33432..34217
                     /gene="scpB"
                     /locus_tag="OQM83_001123"
     CDS             33432..34217
                     /gene="scpB"
                     /locus_tag="OQM83_001123"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCS1519433.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            34241..35719
                     /gene="scpC"
                     /locus_tag="OQM83_001124"
     CDS             34241..35719
                     /gene="scpC"
                     /locus_tag="OQM83_001124"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCS1519434.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
BASE COUNT         8544 a         9613 c         9040 g         8569 t
ORIGIN      
        1 tgagttatgt cttctcaaat ttcgcgatgc aataaacggg catatattag gtagcatgac
       61 cgttttaatt ataattttgc gactgagttc aaattattgc ccctcaatct atgccgcttt
      121 cattatttca acattgccga tcaaaagagc gctatccaga taaaaacgat taaatgcgag
      181 agtgcgacat gccagaatga ttaatgaaat atcaatatga gaaaattaca gataattcca
      241 ttgtcaggtt ctcttgtcat tttcgtggtt taagcacgat tatgtgattg cctccgcaat
      301 tcctgtctct aactcccctt cctcgcaaaa actggcactc cacgagcatg tgtttagaca
      361 gtttcattaa cgtaaacggt tgctttttac tctggcgggc gaaaggagaa acactgatga
      421 gcgccataga ttcccaactt ccctcatctt ctgggcaaga ccgcccaact gatgaggttg
      481 accgcatatt atcaccagga aagctgatca tactcggtct gcaacacgtc cttgtcatgt
      541 acgcaggtgc agtcgctgtt cctcttatga ttggtgaccg actgggcctc tcaaaagatg
      601 ctattgcgat gctcattagc tcggatctct tttgctgcgg gatcgtcaca ttattgcaat
      661 gtatcggtat cggccgcttt atggggatcc gcctgccggt gattatgtcg gtgacctttg
      721 ctgctgtaac accaatgata gccattggga tgaacccgga tatcggcctg ctggggatat
      781 ttggtgccac tatcgccgcg ggttttatca ccacattatt agcgccattt attggtcgct
      841 tgatgccttt attcccgcca ctggttaccg gtgtggttat tacttctatt gggcttagca
      901 tcattcaggt gggtattgac tgggccgccg gaggtaaagg gaatccgcaa tatggtaatc
      961 ccgtttattt aggtatctcc tttgctgtct taatttttat cttgctcatt actcgctatg
     1021 cgaaaggatt tatgtccaac gtcgccgtat tactggggat tgtatttggc tttttacttt
     1081 cgtggatgat gaatgaagtc aatttatccg ggctacatga tgcttcatgg tttgcgattg
     1141 tcacgccgat gtcatttggt atgccgattt tcgatcccgt ttccattctg accatgactg
     1201 ccgtgttaat catcgtgttt atcgagtcga tggggatgtt cctggcactg ggtgaaatag
     1261 tcggtcgtaa actctcttca cacgatatta ttcgcgggct gcgtgtcgat ggcgtaggga
     1321 caatgatagg cggaacgttt aacagcttcc tccacacgtc attttcacaa aacgttggcc
     1381 tggttagcgt gacgcgcgtt catagccgct gggtgtgtat ttcttcggga attatattaa
     1441 tcctgtttgg catggtgcca aaaatggcgg tgctggtcgc ctccattccg caatttgtgc
     1501 tgggcggtgc tgggctggtg atgttcggca tggtactggc gacagggatt cgaattctgt
     1561 cgcgctgtaa ctacaccacc aaccgttaca acctctatat tgtggcgatc agtctcggcg
     1621 ttggcatgac tccgacgctc tctcacgatt tcttttctaa gttaccggcc gtactgcaac
     1681 cgttgctgca tagcggcatt atgctcgcaa cccttagcgc cgttgtgctg aatgtcttct
     1741 ttaatggcta ccagcatcat gctgacctgg tgaaggaatc cgtctctgat aaagatttaa
     1801 aagtcaggac agtacgtatg tggcttctga tgcgcaagct gaagaaaaat gagcatggag
     1861 aataatatga attttttaat gcgcgttata ttcagtctgc tgttgctttt tactctctct
     1921 attcctgtca tttctgactg tgttgcaatg gccattgaaa gtcgcttcaa atatatgatg
     1981 ctactttttt aaatggtttt tacctgtcgg catccgctca aaacgggcgg ttgtcgataa
     2041 acgctcactt ggttaatcat ttcactcttc aattatctat aatgatgagt gatcagaatt
     2101 acatgtgaga aattatgcaa acggaacacg tcattttatt gaatgcacag ggagttccca
     2161 cgggtacgct ggaaaagtat gccgcacaca cggcagacac ccgcttacat ctcgcgttct
     2221 ccagttggct gtttaatgcc aaaggacaat tattagttac ccgccgcgca ctgagcaaaa
     2281 aagcgtggcc tggcgtgtgg actaactcgg tttgtgggca cccacaactg ggagaaagca
     2341 acgaagacgc ggtgatccgc cgttgccgtt atgagcttgg cgtggaaatt acgcctcctg
     2401 aatctatcta tcctgacttt cgctatcgcg ccaccgatcc gaatggcatt gtggaaaatg
     2461 aagtgtgtcc ggtatttgcc gcacgcacca ccagtgcgtt acagatcaac gatgatgaag
     2521 tgatggatta tcaatggtgt gatttagcag atgtattaca cggtattgat gccacaccgt
     2581 gggcgttcag tccgtggatg gtgatgcagg cagccaatag tgaagcaaga aaattgttgt
     2641 ctgctttcgc gcagcacaat taaaaaaccc cgacatttgc cggggttgtg agcataacgt
     2701 aatgcttatt ttaccggacg catcgccggg aacagaataa cgtcgcggat ggtatggctg
     2761 ttggtgaaca gcattaccat acggtcgatg ccaatcccca gacctgctgt cggcggtaag
     2821 ccatgttcca gtgcggtaac gtaatcttca tcgtagaaca tcgcttcgtc gtcacctgcg
     2881 tctttcgcgg caacctgatc caggaagcgt tgcgcctgat cttccgcgtc attcagctcg
     2941 ctaaagccgt taccgatttc acgaccaccg atgaagaact caaagcggtc tgtgatttcc
     3001 gggttaatgt cgttacgacg cgccagcgga gaaacttctg ccggatattc agtaatgaag
     3061 gtcggctgaa tcagatgtgc ttctgccact tcttcgaaga tctcggtaac gatacggccc
     3121 agaccccagc tcttctcaac gtggataccg atagattcag caatcgcttt cgcagagtcg
     3181 aagttatcca gatccgccat gtcggtttcc gggcgatatt tcttgatcgc ttcacgcatg
     3241 gtcagttttt cgaacggttt accgaagtcc agcgtcacgt cgccgtaggt cacttccgtc
     3301 ttaccgagaa tatcctgtgc cagagtacgg aacagcgatt cggtcagctc gatcagatct
     3361 ttgtaatctg cgtaagccat gtagagttcc atcatggtga actctgggtt atgacgtacg
     3421 gaaatacctt cgttacggaa gttacggttg atttcgaata cacgctcgaa gccaccgacc
     3481 accagacgct tgaggtacag ttccggcgcg atacgcaggt acatgtcgag atccagcgcg
     3541 ttatggtggg tgataaacgg acgcgcagcg gcaccgcctg ggatcacctg catcatcggc
     3601 gtttcaactt ccataaagcc gcggttcacc atgaactggc gaataccaga gaggatctgc
     3661 gagcgtactt taaaggtgtt acgggattca tcgttagaga tgagatccag ataacgctga
     3721 cgatagcgcg cttcctgatc ctgcaagccg tggaatttat ccggcagcgg acgcagtgct
     3781 ttggtcagca gacgcagctc ggtacagtgg atagacagtt cgccggtttt ggttttgaac
     3841 agtttacctt tcgcgccgag gatgtcgccg aggtcccatt ttttgaactg ctcgttgtaa
     3901 acgccttccg gcagatcgtc acgggcaacg tacagctgaa tgcggccacc cacatcctgc
     3961 agggtaacga aagacgcttt acccataata cggcgggtca tcatgcggcc agcaacggcg
     4021 acttcgatgt tcagcgcttc cagttcttcg ttctctttac cgtcgaattc tgcgtgcaat
     4081 tggtcagagg tatgatcgcg acggaaatcg ttcgggaagg caatcccctg ctcgcgcagg
     4141 ttcgccagct tctcacgacg cgttttcagt tcattgttaa gatcgactac cgcgtcagcg
     4201 ccctgtgcgt gttgttcaga catgttggtt cctcataacc ctgctttcaa acttgcttcg
     4261 ataaattgat ccaggctgcc gtccagcacg gcctgcgtgt tgcgagtttc taccccggta
     4321 cgcagatctt taatgcggga gtcatcaagg acataagaac gaatctggct gccccagccg
     4381 atgtcagatt tgttatcttc catcgcctgt ttctcggcat ttttcttctg catctccagt
     4441 tcataaagct tcgctttcat ctgcttcatg gcctgatctt tgttcttgtg ctgggaacgg
     4501 tcgttctggc actgggtcac gatcccggtc gggatgtggg taatacgcac cgcagattcg
     4561 gtacggttaa cgtgctgacc gcccgcgccg gacgcgcgat aaacgtcaat gcgcagatcc
     4621 gccgggttga tttcgatatc aatatcatca tcaacttccg gataaacaaa cgcagagctg
     4681 aacgacgtgt ggcgacgacc gccggagtca aacgggctct tacgcaccag gcggtgaacg
     4741 ccagtttctg tacgcagcca gccgtaagcg taatcgccgg agattttgat cgtcacggat
     4801 ttaatacccg ccacttcacc ttccgactct tcgatgattt cagttttgaa accacgcgat
     4861 tctgcccagc gcagatacat acgctcaagc atgctcgccc agtcctgtgc ttccgtaccg
     4921 ccagaccccg cctgaatatc gaggtagcag tcggcgctgt catattcgcc agagaacata
     4981 cggcggaact caagctgcgc cagtttttct tccagggcgt cgagttcagc aacggcttcg
     5041 ttaaaggttt cttcgtcgtc agcttctaca gccagttcca gcagaccaga aacatcttcc
     5101 agcccctgtt tcatttggtc gagggtgtcg acaacggctt cgagggagga acgctcttta
     5161 cccagcgcct gtgcgcgttc gggttcattc cagacatccg gctgttccag ctcggcgttt
     5221 acttcttcca gacgctcttt cttggcgtcg tagtcaaaga taccccctaa gaacgtcgga
     5281 gcgttccgtg aggtcctgaa tgcgattatt taccggatta atttcaaaca tggtctgatt
     5341 tcttttattg agctagtcaa aatgcggtga taagagcggg attgtaccca atccacgctc
     5401 ttttttatag agaagatgac gctaaattgg ccagatattg tcgatgataa tttgcaggct
     5461 gcggttgccg cgaaactcgt tgatatcgag cttgtaagcc agttgcactt cgcgcacgcc
     5521 gttatccggc cagagggcgg tatcgacatt aaaagcaata ccatccagca gcggaccgcc
     5581 gccgaccggt tcgaccatga ctttcaaatg acgttcgccc accagccgct gttgcagcag
     5641 ccggaaatga ccgtcaaaca gcggctccgg gaacatctgc ccccacgggc cagcatcgcg
     5701 cagcagctgc gccacttcca tggtcatttc ggccgggctt aacggaccgt ctgacaccac
     5761 ttcgccttgc aatagcgaag ggtccagcca ctcagtaacc agttcgccaa accgttgttg
     5821 aaagagttcg aatttatccg cttccagcga caaacccgcc gccatcgcat gaccgccaaa
     5881 cttcagcatc atgccagggt agagtgtgtc taatcgctcc agcgcatcac gcatatgcag
     5941 cccctgaatg gagcgaccag aacctttcag cgtaccatca cccgcgggcg caaaggcgat
     6001 aaccggacgg tgaaaacgct ctttaatacg ggaagccaga ataccgacaa ccccctgatg
     6061 ccattcgggg tgatacattg ccagcccgcc gggtagcgtg tcacggctgc gctccagttt
     6121 ctcgcacagg gtcagggctt caacctgcat tccttgttcg atctcttttc gcgtctggtt
     6181 tagcgcatcg agttcatttg ccagcacgcg cgcttcgccg atgttgtcgc acagcaagag
     6241 cgccacaccg acggacatat cgtccagtcg tccggcagca ttgagacgtg gccccagcgc
     6301 aaaacctaaa tcgctggcgg cgagtttttg tgcatcacgg tttgccactt caagcagcgc
     6361 tttaatcccc ggacggcact ttccggcacg gatgcgactc atcccctgcc aggtcagaat
     6421 gcgattatta gcgtccagcg gtacgacgtc cgccactgtc cccagcgcta ccagatccag
     6481 cagttctgcc aggttaggaa ttgcgatgcc gcgctcatca aaccagccct gatcgcgcaa
     6541 aaaggtgcgc agcgccagca tcagataaaa cgccacaccc acgcctgcca gtgatttcga
     6601 cgggaaatta cagtcgcgca agttagggtt aatgatcgct tccgccgcgg gtaatgtttc
     6661 gcccggcaaa tggtgatcgg taacaataac cggaatgccc aacgagcgag cgtgttcaac
     6721 ccccgcatgg gaggaaatac cgttatccac cgtgacaatt aactgcgcac cacgggcatg
     6781 ggcctgatcg accacttccg ggcttaagcc gtaaccgtct tcgaaacggt ttggaaccag
     6841 gtagtcgata ttgctgcaac caagcgagcg catcgccagc acgcttagag ccgtgctggt
     6901 ggcaccgtcg gcgtcgaaat caccgaccac aataatccgc gttccttcgc gaaaggcgtt
     6961 gtaaaggatc tcaacggcct tttcgacgcc gctcagttgc tgccagggca gcatcccttt
     7021 aacactgcgt tccagttctt gcgcactgcg cacgccccgg ctggcgtata aacggcgcag
     7081 caagggaggc aattcagcgg gcaagtctgc cgtttcatcg actgcacggc gacgaagttg
     7141 tatctgttgt ttcacgcgaa ttatttaccg ctggtcattt tttggtgttc gtcgaggaat
     7201 tctttcatct ctttcggcgg ctggtaaccc ggaacaagtg tgccattgct cagcacaact
     7261 gccggagtac cgctaacgcc aagctggacg ccaagtgcgt aatggtcggc aatatccacg
     7321 tcgcagctgg ctggtgcgac gcttttacct gccatcacat catcaaacgc tttgttttta
     7381 tctttcgcac accagatagc tttcatttct ttctctgcat cgctgtccag cccctggcgc
     7441 gggaaagcaa gataacgcac ggtgatcccc agcgcgttat agtctgccat ttgctcatgc
     7501 agtttgtggc agtaaccaca ggtaatatca gtaaacacgg tgatgacgtg tttttcctgc
     7561 agcgctttat aaacgatcat ctctttttca agcgcattca actgctttaa cagcatctta
     7621 ttggtgacat tgaccggagc cgtgccgcta acgtcataca ttggcccctg aatgatatgt
     7681 ttaccatcat cggtgatgta caacacgccg ctgttagtca gaactgtctt catgccagct
     7741 acgggcgcgg gctgaatatc gctgcttttg atgcccattt tggctaacgt ttgttgaatt
     7801 gccgcgtcat cagcctgagc aaaacctgaa aacgccgcta acaaagtaaa caacataaaa
     7861 cctttcttca taaatcttcc cgttcttttc agacatcacg cccgcgggtg atgctgttga
     7921 tgaagttgcc gcagacgctc ggtagcgaca tgcgtataaa tttgcgtggt ggaaagatcg
     7981 ctgtggccca gtagcatctg caccacgcgt aaatccgcac catgatttaa taaatgagtg
     8041 gcaaaagcgt gacgcaacac atgcggtgac agcttttcgc tgtcgatacc cgccagcacg
     8101 gcataatgtt taatgcggtg ccagaaggtc tgtcgcgtca tctgctgcgc acgctggctg
     8161 ggaaacaaca cgtcaattga cacaccattc agcagccacg gacgcccatg ttccagatag
     8221 gtttccagcc agtaaaccgc ctcttcacct aacggtacca ggcgctcttt gttgccttta
     8281 ccaatgaccc gcactacgcc ctgacgcagg ctgatatcac tcattgtcag cccgaccagt
     8341 tcagagacac gcagcccggt agcatacaac acttcaagca tggctttatc gcgtagctcc
     8401 agtggctgat caattaatgg cgcctgtaat aaacgttcga cctgcgcttc gcttaaatct
     8461 tttggcaaac gctggggcaa tttcggtgaa gcgagatgcg cactgggatc gtcttcacga
     8521 aacttttcgc gataaagata ctggaacaat cggcgcactg cactcagcaa acgcgctgag
     8581 ctggtggctt tatacccgcc ctccagccgt tctgccagta atgcctgcaa atcgtcactt
     8641 tgcgccgtcg ccagcgtcaa cccgcggtga tgcaaccact ccaccatcat tgacagatcg
     8701 cgacggtaag cgttcaacgt attttcagcc agattttttt ccagccacag agcatcaaga
     8761 aactgctcga tgcgtgccag atcctgtttc acttgcgccc cttatggtca ctcatttgat
     8821 ccattatgcc ttattgtgcc gtgactaaag cgattctgat acactagctg caaaagccac
     8881 agcagaatcg agaagcttac gttatgaata tgggtctttt ttacggttcc agcacctgtt
     8941 acaccgaaat ggcggcagaa aaaatccgcg atattatcgg cccagaactg gtgaccttac
     9001 ataacctcaa ggacgactcc ccgaaattaa tggagcagta cgatgtgctc attctgggta
     9061 tcccgacctg ggattttggt gaaatccagg aagactggga agccgtctgg gatcagctcg
     9121 acgacctgaa ccttgaaggt aaaattgttg cgctgtatgg gcttggcgat caactgggat
     9181 acggcgagtg gttcctcgat gcgctcggta tgctgcatga caaactctcg accaaaggcg
     9241 tgaagttcgt cggctactgg ccaacggaag gatatgaatt taccagcccg aaaccggtga
     9301 ttgctgacgg gcaactgttc gtgggtctgg cgctggatga aactaaccag tatgacctta
     9361 gcgacgagcg tattcagagc tggtgcgagc aaatcctcaa cgaaatggca gagcattacg
     9421 cctgatctca ctgacggctt agcgcatatg ctttgccgtc atctttatct ttgcgtctct
     9481 tgttgcaaca aaatccgccg taaatcccgc cattcggctt cgtccatgct gtcggctgcc
     9541 agccataaat gttgccgttt accgccatca gaacgtaaac gcagcatcat gccgctctta
     9601 atcatccacg gtgctttgac gatgctccac tcctgccctt gccaacgcaa acgcccgtcc
     9661 atcaacaagc gaatttcccc ctggcgagca ttaatacgcc gctggctgcg aacgcaatca
     9721 aacaccacca gcgaaagtaa caccatccat aacggggtgt aactgagtgg ccagggcatg
     9781 agtaaaataa cagcggcaac cagcccatga atcagcaagg aaagccactg tgcgcgccag
     9841 gagacgcgca aatcagattg ccacaggacc acgttcccgg ttccgtgtct ggatgagtcg
     9901 gaccatcatt tccagttctg catcggccgg tttaccgtga ttcatcagcc agttaaacag
     9961 gtccggatcg tcacattcca gcagacgaat aaagatgcgt ttttcgtcat cgcttaagct
    10021 gtcgtactca tgttcgaaaa acggcatgat tgaaatatcg agttcgcgca taccacggcg
    10081 gcatgcccaa tgaatgcggg ctttgttgtt aatgtccatc ttcttcctgt ctcacgaaaa
    10141 tccagtaccc cgctattgta acgtgttttt cgccttcttt tacgggaata tcagtaaaca
    10201 ccatcgcgat cacgaaatta attcacaaca attcaatggc ttcatttttt tggaagtcgc
    10261 ctcgcagaag gcacagatcg cgtagtgaaa gcacttgcat tgcctcatag ctcttttacc
    10321 attagtcatt aatacgccgt taagcaactc aggactctat tatggctttt acaccttttc
    10381 ctccccgtca gcctacggct tctgcccgtt taccgctgac gctgatgacg cttgatgact
    10441 gggcgcttgc caccattact ggcgcggaca gcgaaaaata tatgcagggt caggtgacag
    10501 cagatgtcag ccagatggca gaagatcagc acctgctcgc cgcccattgc gacgccaaag
    10561 gcaaaatgtg gagcaattta cgtctgttcc gcgacggcga tggctttgcg tggattgaac
    10621 ggcgcagcgt gcgtgaaccg cagctgactg aactgaaaaa atatgcggtg ttctctaaag
    10681 tgaccatcgc gccagacgac gagcatgtgc tgcttggtgt tgccggtttt caggcgcgcg
    10741 ccgcgctggc aaatatcttt agtgaactgc cttcgaaaga aaaacaggta gttaaagaag
    10801 gcgcgaccac tttgctatgg tttgaatacc cggcagaacg tttcctgatc gtaaccgatg
    10861 aagctactgc taatatgctg accgataaac tgcgcggtga agcggaactg aacaatagcc
    10921 aacagtggct ggcattaaac attgaagcgg gtttcccggt gattgatgcc gccaacagcg
    10981 ggcagtttat cccacaggcg accaatctcc aggcgctggg cggtatcagc tttaagaaag
    11041 gctgttatac cggacaagag atggtggcgc gagcaaaatt ccgtggtgcc aataaacgtg
    11101 cgctctggtt gctggcaggt agcgccagcc gactgccgga agctggtgaa gacttagagc
    11161 tgaaaatggg cgagaactgg cgtcgtaccg gtacggtgct ggctgcggta aaactggaag
    11221 atggtcaggt cgtggtacag gtcgtcatga ataacgatat ggaaccggat agcatcttcc
    11281 gcgtacgcga cgatgcgaat acattgcata ttgagccgct gccgtattcg ctcgaagagt
    11341 aaatctcttt atcgcatcag gcatttatcg cctgatgcga cgctgacgcg tcttatcatg
    11401 tctgggattt gatgccttta tcacaacagg gacgaggccc gtagatcgga taaggcgttc
    11461 acgccgaatc cggcagttgt gctccgatgc ctgatgcgac gctgacgcgt cttttcaggc
    11521 ctacaaaggc atacccatta cgcctgccca atatacaaat agatcgccag aaagtggcac
    11581 acactgccgc cgagcacgaa gccgtgccag atggcatgat tgtagggaat gcgtttgcag
    11641 acatagaaaa tcactccgag cgaataaacc acgccgccta ccgccaataa ggtaacgctg
    11701 cccgccgcga gcttaactgc catttcataa attaccacca gcgacagcca gcccatcgcc
    11761 agataggtca ccagagataa aattttgaat cggtgcgcga tggtcagttt aaacagaata
    11821 cccagcaatg ccaggctcca gataacaatc atcaacccgc gcgctaacgg agaatccagc
    11881 cccaccagca aaaacggcgt gtaggttccg gcaatcaaca ggtaaatagc gcaatggtca
    11941 aatttcttca gccacatttt tgcccgttga tgaggaatgg cgtgatagag cgtcgaagcg
    12001 aggaacagca ggatcatact gccgccatag aggctgtaac tggttatcgc tgtggcgctg
    12061 gcattgagat ccaccgcctg aaccagtagc aacaccagcc caacgatacc aaacaccaac
    12121 ccaatgccgt gactgacgct gttggcaatt tcctctgcca gcgaatatcc ctgcttaatg
    12181 aggggcttct gaaccataac ttactccgga gaaacgtaca cgcacatgta tacctctcca
    12241 gcgtaactga gaatagttcc agtgaacacc tgttagctaa aataaattct gatttgagac
    12301 taatctccta aaaatcatga aattaaatgc gaaatttcaa ctaacaggcg tgagttcaat
    12361 ttaaagacat ttaaattcaa tcacataaaa ctgtgtctga ccgggataga tgtcggcaat
    12421 gacctttatc agttcagtca gggtcatatt ttcctgctct gcatgttttt ccgtcagcgt
    12481 atccagcgtt acggttgagg ttgcggtgac ttcaatcgtg caaaaataac cgtcatcttc
    12541 aaaacgcccg acacgaagca catcacccgt tttgaagtgc gattcagact cgtcgcggat
    12601 ggtgatggtt ttacgcccag ccagaatgtc atcctggaaa cgttgaaaaa aagtgatgtc
    12661 gtttggctgc atggtactat ttcctgtaag aattgactca tctggagcct atgatagtga
    12721 aaaaactcac cttacagaaa gatttcttat ggggcggcgc agttgccgct catcaggtcg
    12781 aaggcggctg gaacaaaggc ggcaaagggc cgagcatttg tgacgttctg accggtggcg
    12841 cacacggcgt gccgcgcgaa atcaccaaag aagtcttgcc aggcaaatac tatccaaacc
    12901 atgaagccgt tgatttttat ggtcactaca aggaagacat caagctattt gccgaaatgg
    12961 gcttcaaatg ttttcggact tccatagcct ggacgcgcat ttttccaaaa ggcgatgaag
    13021 ctcagccaaa cgaagaaggg ctgaagttct acgatgatat gttcgatgaa ctgctgaaat
    13081 acaacatcga accggtgatc accctctccc actttgaaat gccgctgcat ctggtgcagc
    13141 aatacggtag ctggaccaac cgtaaagtgg ttgatttctt tgtccgtttc gcggaagtgg
    13201 tgtttgaacg ctataagcat aaagtcaaat actggatgac cttcaacgaa attaacaacc
    13261 agcgtaactg gcgtgcaccg ctgttcggtt actgctgctc cggcgtggtt tataccgagc
    13321 atgagaaccc ggaagagacg atgtatcagg tgctgcatca ccagtttgtc gccagcgccc
    13381 tggcggtgaa agctgcgcgt cgcattaacc cggagatgaa agtcggctgt atgctggcga
    13441 tggtgccgct ctatccctac tgctgtaacc cggacgatgt gatgttcgct caggagtcga
    13501 tgcgcgaacg ctacgtcttt accgatgtgc agctacgcgg ctattacccg tcctatgtgt
    13561 tgaacgagtg ggagcgtcgc ggatttaaca tcaaaatgga agacggcgat ctcgatgtac
    13621 tgcgcgaagg cacctgcgat tatcttggtt tcagctatta catgaccaac gcagtgaagg
    13681 ccgaaggcgg caccggcgat gcgatctctg gtttcgaagg cagcgtaccg aacccgtatg
    13741 ttaaagcgtc tgactggggc tggcagattg atccagtagg tctgcgctat gccctttgcg
    13801 aactgtatga gcgttatcag aggccgctgt ttattgtcga aaacggtttt ggcgcttacg
    13861 acaaagtgga agaagatggc agcatcaacg acgactaccg cattgactac ctgcgcgccc
    13921 atatcgaaga gatgaaaaaa gcggtgactt acgatggcgt ggatctgatg ggctacacac
    13981 cgtggggctg catcgactgc gtatcgttca ccaccgggca gtacagcaaa cgctacggct
    14041 ttatctatgt gaataaacat gacgacggta ctggcgatat gtcgcgttca cgtaagaaga
    14101 gctttaactg gtacaaagag gtgattacca gcaacggcga gaagctttaa gtcgatgaag
    14161 taccggatgc aatacttgtt gcatccggtc agacaacttc cctgttttat ttcccgcccg
    14221 ccaaatcgat aaaacttccc gtgacgtaag aggctttatc acttagtagc cagacaatgg
    14281 cctgcgcgac ctcttctgcc tgtccaccac gctgcatggg gatgttcgac ttaacgcgat
    14341 cgacgcgtcc aggctcgccg ccgctggcgt gcatttcggt aaaaataaac cctggccgca
    14401 cgcagttaac gcggaccccc tgcgcggcga cttccagcga tagtccggtg gttaacgtat
    14461 caatcgcccc tttcgatgcc gcgtaatcaa catattcccc tggcgaaccc aaccgtgagg
    14521 ccaccgaaaa gacattgacg atagcgccgc cactgccacc atttttaagc gccatgcgtt
    14581 ttaccgcctc gcggcagcag agaaaatatc ccgtcatgtt ggtggaaagt actcggttga
    14641 ttcgctctgc ggtaaggttt tcaacggtgc actgggtaaa caagatcccg gcgttattga
    14701 ccagcgctgc tagcggttca tcgtgctgat cgattgctgt aaacattgcg atgacctgat
    14761 tttcgtcgct gatatccgcc tggagcacga atgccttgcc accggcttgc gtgattaagt
    14821 tcaccacttc ctgcgccgca tggaggtttt gttgataatt aaccgccacc gtatactctt
    14881 ctttcgccaa cagtaatgca gttgcccgcc cgatgccgcg actgccatcg gtcacaagtg
    14941 ctatagccat ttgtttttcc gagaaaaatt aattcgttga cgtatctttg agattgctaa
    15001 caaactggct caatcttcaa tgttggcacg cttttcccct cgccctttca gggagagggc
    15061 cggggtgagg gtaaatattc gcgccagtgc cggcctgttc ccctcaccct aaccctctcc
    15121 ccaaaggggc gaggggactg tctgagcact tttgtacttt gtcatctgac taaaaaggcg
    15181 ccgaagcgcc tttagaaaat agtcgaatca gtgaattact ggtattcgct aatcggtacg
    15241 caggagcaga acaggttacg gtcgccgtaa acatcatcca gacgtttcac cgtcggccag
    15301 tatttgtctg ccacgcctgc cgggaatacc gcaacttcac ggctgtacgg atgcgcccac
    15361 tcggcgacca gttcgctctg aatgtgcggc gcgttcacca gcgggttatc ttccagcggc
    15421 cagacagcgg ctttcacctg gtcaatttct gcgcggatag ccagcatcgc gtcgataaag
    15481 cgatccagtt ccactttgct ttcagattca gtcggttcaa ccatcagcgt acccgccacc
    15541 gggaacgaca tcgtcggcgc gtggaaaccg tagtcgatca ggcgcttggc aatatccagc
    15601 tcgctgatgc cggtttcttc tttcagcggg cgaatatcga gaatacattc gtgcgccacg
    15661 cgaccgtcgc gaccggtata cagcaccggg aaggcatcct gcaggcggct ggcaatatag
    15721 ttggcgttga ggattgccac ctggcttgct tttttcagcc cttctgcgcc catcatgcgg
    15781 atgtacatcc agctgattgg caggatagag gcgctaccga acggtgccgc agaaactgcg
    15841 ccctgacggg ttaacatgcc ttcgatttgc accacgctat gacccggtac aaacggtgcc
    15901 agatgcgctt tcacaccgat cggtcccata cccggaccac caccgccgtg cggaatgcag
    15961 aaagttttat gcaggttgag gtgcgaaacg tccgcgccaa taaagcccgg cgaggtgatg
    16021 ccaacctggg cgttcatgtt cgcgccatca aggtaaacct gaccgccgaa ctgatgcacg
    16081 acttcacaca cttcacggat cgtttcttca tacacgccgt gggtagaagg ataagtcacc
    16141 atgatacagg agaggttatc gcccgcctgt tccgctttcg cgcgcagatc agtcagatcg
    16201 atgttgccgt ttttatcaca cgccacaacc accacctgca ttcctgccat atgtgcagaa
    16261 gcggggttag ttccgtgcgc agaagccggg atcaggcaga tatcgcgatg cccttcgttg
    16321 cggctttcat gataatgacg aatcgccagc aggcccgcgt attcgccctg tgcgccggag
    16381 ttcggctgca tacaaacggc gtcgtaaccg gtcagtttca ccagccagtc agccagctgc
    16441 gcaatcatct gctgataacc ttcagcctgt tccggcgggc aaaacgggtg cagttcggca
    16501 aattccggcc aggtgattgg gatcatctcg gcggcggcgt tcagtttcat ggtgcaggaa
    16561 cccagcggga tcatcgcctg attcagcgcc agatctttac gctccagcga gtgcatatag
    16621 cgcatcattt cggtttcgct gtggtagcga ttaaacaccg gatgggtgag gatttcgtcg
    16681 tcgcgcagca tcgcaggctg gatagagcgg ctgtcgtgag ccacgtcttt gtccagcgtg
    16741 tcgatctcca gcccgtggtt atcgcccagc agcacgctga aaagctgcat tacgttttca
    16801 cgcgtggtgg tttcatcaag ggtgatcccc acggcgttca ggatatcgct acgcaggttg
    16861 atttcagccg cttcggcacg cgtcagtacg cccgctttgt cggccacttc cacacacaag
    16921 gtgtcgaaat agtgcgcatg gcgcagtttc agaccttttt gttgcaggcc cgccgccaga
    16981 atatcggtca gacggtgaat gcggttagcg atacgtttca ggccaaccgg gccgtgataa
    17041 acggcataca ggctggcgat gtttgccagc agtacctggg aagtacaaat gttggagttc
    17101 gctttctcac gacgaatatg ttgctcgcga gtctgcatcg ccatgcgcag cgcggtattg
    17161 ccagctgcat ctttcgatac accgataata cggcccggca ttgagcgttt gtattcatct
    17221 ttcgccgcaa agaatgccgc gtgtgggcca ccgtagccca tcggcacgcc gaagcgttgc
    17281 gccgaaccaa aaacaatatc cgcgccctgt ttacccggcg cagttaacag caccagcgcc
    17341 ataatatcgg cggcaacgct gaccacaatt ttgcgtgatt tcagttcgct aataagcgca
    17401 gtgtagtcgt gaatttcacc ggtagtgcct acctgctgta acagcacgcc gaagacgtcc
    17461 tgatggtcga gcactttttg cgcgtcatcg acaatcactt caaaaccaaa ggtttcggca
    17521 cgagtacgga ccacatccag cgtttgcgga tgcacatcgg aagccacgaa gaagcggttg
    17581 gcatttttca gtttgctgac gcgtttcgcc atcgccattg cttcggcggc agcggtggcc
    17641 tcgtccagaa gagaagcaga ggccatatcc agtccagtca aatccagcgt tacctgctgg
    17701 aagttgagca gtgcttcaag gcggccctgg gagacttcag gttgatacgg agtgtacgcg
    17761 gtataccagc ccggattttc cagcatgtta cgcaggataa ccggcggtag ctgcacggcg
    17821 gtgtaaccca tgccgatgta agacgtgaag cgtttattgc gactggcaat agccttgagt
    17881 tctgccagtg cggcgtattc ggtcgccggt gcgccaacct gcggcggggt cgcaagctga
    17941 atatctttcg gtacaatctg gccggtcagc gcgtttaacg attgtgcgcc aacggcattc
    18001 agcatttctt gctgttgcgc ggcgtccggt ccgatatggc gttcaataaa agcgccgctg
    18061 ttttcaagct ggcttaacgt ctgtgtcatg agcgatggtt cctgaaacgt gcagtgaatt
    18121 gtgaacctct ctccttacga agagagtgag ggtgaggcat aaattttcct caccctgatc
    18181 ctctcccgca gaagaggaat aaagccgtta ctcgtcttct aacaatgctt cgtatgcggt
    18241 cgcatccagc agtgattcca gttcgctttc atcgctggct ttgattttaa agatccagcc
    18301 acctgcatac ggttcgctgt tcaccagttc cggggaatcg ctcagtgcgt cgtttaccgc
    18361 cacgatttca ccgcttactg gcgcataaat gtctgacgcc gcttttactg attcggcaac
    18421 cgcgcagtca tcgcccgcgc taaccgttgc gcccacttcc ggcaggtcaa caaacaccat
    18481 atcgcctaac agctcctgag catgttcggt aataccaacg gtgtaagtgc cgtcggcttc
    18541 tttacgcagc cattcgtgtt ctttgctgta tttcagttct gctggtacgt tgctcatcaa
    18601 tcaatctcca aaaaagtaaa tcacgcgacg gctttgccgt tacgcacaaa aacaggtttt
    18661 gtcactttaa ccgacatttc acggttgcga atttgcacaa tcgccgtttc gccaatacct
    18721 tccagcacgc gcgccagcgc aatgctgtaa cccagcgtcg gggagaaagt accgctggtg
    18781 ataatgcctt catgctggtt gccctgcgca tcggtaaagc gtaccggcag ttcattacgc
    18841 agcacgcctt tttcggtcat caccagacca accagttttt ctgtaccatg ctcacgctgc
    18901 acttccaggg cttcacgacc gataaagtca cgatctgccg gttcccaggc gatagtccag
    18961 cccatgttgg cggctaaagg agaaatagtt tcgtccatct cctgaccata aagattcatg
    19021 cccgcttcca gacgcagcgt gtcacgcgcg cccaagccac atggcttaac acccgcttcc
    19081 accagcgcac gccagaaatc ggccgctttt tcattgggca gcgcaatttc atagcccgct
    19141 tcaccggtat aaccagtggt ggcaataaac agatcgcccg cctgcacgcc aaagaacggt
    19201 ttcatccctt ccaccgcctg acgctgggcg tcattaaaca gtgtggcagc ttttgcctgc
    19261 gcattcggcc cttgcacggc aatcatggaa aggtcatcac gaacggtaat ttcgatgccg
    19321 aaaggttcag cgtgttgggt aatccaggag aggtcttttt cgcgggtggc ggagttaaca
    19381 acgaggcgga agaaatcttc agtaaagtag tagacgatga ggtcatctat cacaccgcca
    19441 gaggcattca acatccccga gtaaagggct ttgccgcttt tggtgagctt cgccacatcg
    19501 ttcgccagca gataacgcag aaactcccgg gtgcggctgc cgcggagatc gacgatggtc
    19561 atatgtgaca catcaaacat tccggcatcg gtacgtaccg catgatgttc gtcgatttgc
    19621 gaaccgtaat gcagcggcat catccagccg tggaaatcca ccatgcgagc gccgcaaagc
    19681 gtgtgttgtt cgtacaaagg agtctgttgt gccatcttgt cctcattgaa taagcggggc
    19741 tgacaacttt tcatggtgaa attatcacca cgaaacccag catcggagcc actcccggtc
    19801 cccaacgcaa tcgttctctt ttgcctgaac ttaccaccga aacagactgt taaccataag
    19861 gtaaaattga tcatcacatt agcttatggt taaaaaacgc caaaatcgcg acagaataaa
    19921 aaaccaaaaa acacaccagt ttctatataa agatgatgcc attagaaagc taatttgaat
    19981 aaggcaatat taagagctaa aaaaatgtca aaaaacacta aatcaaaaaa taatgacatt
    20041 agaaaatata atgcgaaaac ggaggtgaaa ttagtttatt tcaaatgagg aaaatctccc
    20101 ggcgaaaaaa ccgggagatg aaagtgtgat gggtatcaaa taaacagcag aagagaaatt
    20161 tttaacgcag ccattcaggc aaatcgttta atcccattgc ctggcggata agttgtggtt
    20221 taacgccagg aagcgtgtcg gccagtttca aaccaatatc acgcagcagt tttttcgccg
    20281 gattggtacc ggaaaacaga tcgcggaatc cctgcatacc agccagcatc agcgccgcac
    20341 tgtgcttgcg gctacgctca taccggcgca ggtaaatgta ctgcccgatg tctttcccct
    20401 gacgatgcaa ccgtttcagt tcagcaatca gctctgcagc atccataaag ccaagattta
    20461 ctccctgccc cgccagcggg tgaatggtat gcgcggcgtc gcccaccagc accagacggt
    20521 gcgcggcaaa ctggcgcgca taacgccccg tcagtgggaa cacctgacgc gcgctctcaa
    20581 ccttgcataa gcccaggcga ttatcaaaag cgatatttaa cgcgcgatta aattcatctt
    20641 cacttgcctg ctgcatccgc tgcgcttcct ctggcgacag tgaccagaca attgagcaaa
    20701 gatgcggatc gctaagcggt aaaaaggcca gaatgccttc gccatggaaa acctgccgcg
    20761 ccaccgcatc atgcggttct tccgtgcgaa tggtcgctac cagcgcgtga tgctgataat
    20821 cccagaaagt cagcggaata tctgctttgt tgcgcaacca ggaattagca ccgtccgcgc
    20881 caatcaccag acgcgccgtt aacatgctgc catctttcag cgtcaggaaa gtttcatttt
    20941 ctccccaggc gacctgctgt aattctgcgg gggccaacag agtgatatct gacgactgct
    21001 gcgctttgtt ccacagcgcg tagtgaatca ctgagttttc aacgatatgc ccaagatggc
    21061 tatagcccat gctttgatcg tcaaacgaaa tgtgaccaaa actgtctttg tcccacactt
    21121 ccataccgtg ataacaactg gccctacgag agagaatgtc ctgccagacg ccaagacggg
    21181 tgagtaattt ttcgctggcg gcattgatag ccgaaacgcg cagttgtggt ggtgcatccg
    21241 ccgccagagg ttccggtacg cgctgctcca gtacggcaac gcgtaagccg ctcccctgta
    21301 agccgcaggc aaccgccagc cccaccatgc cgccaccaac aatggctaca tcaacacttt
    21361 gcattgttta ttccttaaaa ccgccttcaa cgcgccaccc aaccgagggt gcgctgcgcc
    21421 agcacatcgc gtgccggggt gaataattcc atcgtcatca gtccgatgtt gcgcccgaca
    21481 accagcggtg cccaacggtt ggcaaaaaga tgtaccaggc tgtccgtgac gccgatggtt
    21541 gcttcgcgat cgctctgtcg acgctgctga tagcggcaca atatgccgta atcaccgatg
    21601 tcttctccgc gctcatgcgc ctgagtcagg gtttccgcaa ggctcatgac atctcgcata
    21661 ccgaggttaa acccttgccc ggcaatcggg tgcagagttt gcgccgcatt gcccaccagc
    21721 acggtacgat gggtaatagc tctggcggcg cgggttaacg ccagcggata agcactgcgt
    21781 ttaccggcgt gggtaatttt tccaagtcgc cagccaaagg ccgactggag ttcacggcaa
    21841 aacttctcgt cgctccacga cagcacctct tcgcgccgtt ccagtggatg acaccagacc
    21901 agcgaacagc gtccgtcaga catcggcaac atcgccagcg ggccatgttg cgtgaagcgt
    21961 tcaaaagcgc ggccttcgtg cgcaacggaa gtcgcaacgt tggcaatcac ggccagttgt
    22021 tcgtaaggct cctgctgcca gtcaacgccg cacgcggtgg ctaacgctga atgggtgcca
    22081 tcagctgcta ccagcacgcg gcccgtcagc gtctcgccac tctccagcgt cacttcaacg
    22141 tgactctgag tacgggcaac gttagccacg cgatcagggc aatgcagcgt tacgccaggt
    22201 gctttacgca gcaatgcaaa cagccgttgc ccgacattgt gcaattcgac aacctgtccc
    22261 agcgccgcca gttggtaatc ttctgcggcg agggtgacaa atccagcgtg accacgatcg
    22321 ctgacatgca cggtagtgat ggcggttgcg taatccgcca gagattgcca gacgccgatg
    22381 cgcgccagtt gctgacaggt ccccgcagcc agcgcaatag ctcgtccatc aaagcccgga
    22441 tgagcatgtg actctggcgc ggtcgcttca atcaaatgta ccggcagcgc cccgtgactt
    22501 aaccgggaaa tagccagcgc cagcgtcgcg cccgccatgc cgccaccgac gatgattacg
    22561 ctcattgctt tctcgcagct gccatcaacg cttcgatttc ttccggcttt ttcaccacgc
    22621 tggcggtgag gttttcgttg ccggtttcgg taatcacaat gtcgtcttca atacgaatgc
    22681 cgataccgcg atattgttct ggcacatccg catccggcgc gatatacaga ccaggctcta
    22741 cggtcagtac catccccggt tccagaatgc gcgagcgatc ctgaccataa acgccaacgt
    22801 catggacatc cagtcctaac cagtggctaa ggccatgcat aaagaaagga cgatgggcgt
    22861 tctgagcgat cagttcatca acatcacctt tcaggatgcc gagttttacc aggccgctaa
    22921 ccatgatgcg caccacttca ccagtgactt ccagaatgga agttcccgga cgatacaggc
    22981 gcaggctggt ttcgagagac tccagcacaa tgtcgtagat ttcacgctgg gcctgggtga
    23041 atttgccgtt gaccgggaag gtgcgggtaa tatcgcccgc gtaacctttg tattcacaac
    23101 ccgcgtcaat caacaccagg tcaccgtcgc gcagttcact ctcgttttcg gtgtagtgca
    23161 gaatgcagcc gttttcaccg ctgccgacaa tggtgttata ggacggatag cgcgcaccgt
    23221 ggcggttaaa ttcgtggtga atttcgcctt ccagatggta ctcgaacatt cccggacggc
    23281 atttttccat cgcccgtgta tgtgccatgg cggtgatttc tcccgcgcgg cggagtacgg
    23341 caatctcttc tggcgatttg aacaggcgca tttcatgaac aacaggacgc cagtcgatca
    23401 tcgttgccgg tgcggtgaga ttttgccgcg aacctttacg cagtttttcc agcgcactgt
    23461 tcatgatttc atcagcatat gcatattcgc cctgggcatg gtaaaccaca tccaggccgt
    23521 taagtagttg ataaagttgc tgattgattt cgctgaatgc cagtgcgcgg tcaacgccca
    23581 gtttctctgg cgcggcatcc tggcctaagc gacggccaaa ccagatctcc gccgtcaggt
    23641 cgcgaacgcg gttaaacaga acgctgtggt tatgagtgtc atcgctttta atcagcacca
    23701 gcaccgcttc caattcgtta aagccggtga agtaccagaa gtcactgttc tgacgatagg
    23761 ggtattcgct gtcggcgcta cgtgttactt ctggtgcagc aaaaatcagc gcggcgctgc
    23821 cgggttgcat ttgctccacc agggcctgac ggcgacgctg aaactcttgc cgggatatct
    23881 cactcataac actctcctta cgttttttgt ttttagtgta gagtcggttt ttgtacttct
    23941 ggcgcggtcg gttgcggatg agtaaaggtg tcgtggcata acagcgcggc aacacgaacg
    24001 tattcgatga tctcttcaag cgacatttca agctcttcct gatcttcgtc ttcgtcgtaa
    24061 cccagttgcg caatgttacg cagatcgtcg atagcttcac cggtttcgcc ggtcacttta
    24121 tccagcttcg gttgcgtaac gccaagacca agcaggaagt gattgaccca acctgccaat
    24181 gcatcagccc gatcgaaaac gctgacatca tcgccatcag gcagataaag ctgaaaaagg
    24241 aagccgtcat cctgcagggc atcgctggtg gcagagtgca ttttacgcag tgcctgtgcc
    24301 agctcatgac cgaaagccat gccttcgttc gtcaggtcgt gaagtagcgg cagccatgag
    24361 ctgtcatcgt taccgccaca tatcatccca ctgattaaac catgcatctc ggcaggggtc
    24421 agccccgtcc cttgttggtt cagatactgg ttcatttcgt tgtaaccagg catttcgttc
    24481 tgtatagaca taagcattcg tcgtcaaagg gaggaatatt catgatatgc taccactttg
    24541 ggccctggtg gaccagaaaa gggcttgtct cttctcatca gggtagctat agtgtcgccc
    24601 cttcgcagac catgggtcta aagacgaagg cagcgcagtc aatcagcagg aaggtggcat
    24661 gtctgcacaa cccgtcgata tccaaatttt tggccgttca ctgcgtgtga actgcccgcc
    24721 tgaccaaagg gatgcgttga atcaggcagc ggacgatctg aaccaacggt tgcaagatct
    24781 gaaagaacgc actagagtca caaatactga acagttggtc ttcattgccg cattgaatat
    24841 cagctatgag ttagcgcaag aaaaagcgaa gactcgtgac tacgcggcaa gtatggaaca
    24901 gcgtattcgg atgctgcagc agaccataga acaagcgtta cttgaacaag gtcgcatcac
    24961 cgaaaaaact aaccaaaact ttgaatgaca cttttcggtt tactgtggta gagtaaccgt
    25021 gaagacaaaa tttctctgag atgttcgcaa gcgggccagt cccctgagcc gatatttcat
    25081 accacaagaa tgtggcgctc cgcggttggt gagcatgctc ggtccgtccg agaagcctta
    25141 aaactgcgac gacacattca ccttgaacca agggttcaag ggttacagcc tgcggcggca
    25201 tctcggagat tcccttctta tctggcacca gccatgacgc aactaccaga actcccactg
    25261 acattatccc gacaagaaat ccgcaaaatg attcggcaac gtcgtcgtgc gttaacgccg
    25321 gaacaacagc aggaaatggg tcaacaagcc gctacccgga tgatgactta tcccccggtg
    25381 gtgatggcgc atacggtcgc tgtattcctc tcttttgatg gtgaactcga cacccagccg
    25441 ctaatagaac aactctggcg cgccggtaag cgcgtatatc ttccagtttt gcatcccttt
    25501 agtgccggta atttgctgtt cctgaattac catccgcaaa gcgaactggt gatgaacagg
    25561 ttgaagatcc atgagccaaa actggatgtg cgtgacgtgc tacccctttc ccgattagac
    25621 gtgctgatca caccgctggt cgcctttgat gagtacggtc agcgcctggg aatgggcggt
    25681 ggtttttatg atcggacctt acaaaactgg cagcactata aaacgcaacc ggtgggttat
    25741 gcgcatgatt gtcagttggt ggaaaaactc cccgttgaag agtgggatat ccctcttcct
    25801 gcggtggtta caccgtcgaa agtctgggag tggtaagggc gatacacctg catcgccctg
    25861 attgacatcg ttgattcttt aacctaattt agtgagtaag ggtaagggag gattgctcct
    25921 cccctgtgac tgactgttaa taagctggga aacttatgag taacagtaca atcagtatga
    25981 tggcaagtcg catcataacc cttctccttc aagccctcgc ttcggtgagg gctttaccgt
    26041 tacagcccca tgctgcccag ctatcgtaag ctctctttaa ataaccacaa cgcattgatc
    26101 tacctttaaa tcttttcttc gagcgtgctc gaaaaaccca tgcaaaacag agataatttt
    26161 tgggaaaaag gatctattac ggcagtaaac gtgggcgatg aagtggctat caaaaagttc
    26221 ggttcagggc gatgcacccc gcatcgccct gattgacatc gttgattctt tgacctaatt
    26281 tagtgagtaa gggtaagaga ggatgactcc tcccctgaga ctgactgtta ataagctccg
    26341 aaacttatca gtaacagtac aatcagtatg atgacaagtc gcatcataac ccttctcctt
    26401 taagccctcg cttcggtgag ggctttaccg ttacagcccc atgctgtccc gccatcgtaa
    26461 atccccatta aataaacaca acgcattgat ctgagtttga tttattttct ggagcagact
    26521 cgcaaagtag aatgcgcaac gcggcaacgg tgtggagaag ggataaaaaa acgggcaagt
    26581 cagtgacctg cccgttaatt ttcagagaag gggaattagt acagcagacg ggcgcgaatg
    26641 gtacccggaa tagctttcat tgcctgcagc gctttttcgg caacgtcttc gtcagcttca
    26701 atatcaataa ctacataacc catctgggcg gaagtttgta gatattgcgc ggcgatgttg
    26761 acgccctgct cggcgaagat tttgttcagc gcagttagca cgcccggacg gttttcgtgg
    26821 atgtgcatca gacgacgacc accatgcagt ggcagcgaga cttccgggaa gttcaccgca
    26881 gagagcgttg agccattgtc agaatacttg atcaatttac ccgcaacttc caggccgata
    26941 ttctcctgcg cttcctgagt cgaaccgcca atgtgtggcg tcagaaggac gttgtcgaat
    27001 tcacacagtg gagaggtaaa tggatcgcta ttggtcgccg gttccgtcgg gaatacgtcg
    27061 attgccgccc ccgccagatg tttgctcgcc agcgcatcac acagcgccgg aatatccacc
    27121 acagtaccgc gcgaggcatt aatcagcagc gagccgggct tcattagcga aatctctttc
    27181 gcgcccatca tatttttggt ggacggattc tctggtacat gcagactcac cacatcgctc
    27241 atattcagca ggtcagaaag atgctgtacc tgagtggcgt tgcccagcgg cagtttgttt
    27301 tcaatatcat aaaagtaaac atacattccc agcgattcag ccagaatgcc caattgcgta
    27361 ccaatatgac cgtagccgat gatacccagc tttttgccgc gcgcttcaaa agaacccgcc
    27421 gccagtttgt tccacacgcc acggtgcgct ttagcattgg cttccggcac gccgcgcaat
    27481 agcagcagca gttcgccaat caccagctcc gcaacagagc gcgtatttga gaacggtgcg
    27541 ttaaataccg ggatcccgcg ctttgccgcc gcatccagat caacctggtt tgttccgata
    27601 cagaaacagc caatagcgac cagtttttct gcggcgttga tcacgtcttc agtcagatgg
    27661 gtacgggatc gcaggccgat gaagtgggca tcgcggatgg attcttttaa ttgttcatca
    27721 tccagcgcgc ctttgtgaaa ttcgatgttg gtgtaaccag ctgcacgaag gctttccagc
    27781 gccttttggt gcacgccttc taccagcaga aacttaatct tgtctttctc cagcgatacc
    27841 tttgccattt acccaatcct gtcttttgaa atgttgtgtg cggatttgca tccgcctttc
    27901 aacatatcaa aaaataatat tgcggcaata tgaacgtttg cgtcgcgatg ttgaggaaat
    27961 atcacgcaaa ggtatttctt ggttgaatat gctggtttag aggatttatt tagagcaatc
    28021 gacaatagcc aagtaaaaac gtgacacatg tcaccaaatt taatgaagag aattttttta
    28081 acgggggagg ttcccccgtc agatcatttc acaatggttt tgacaccgtc aggtgtgcca
    28141 atcagcgcaa cgtccgcgcc acggttagca aacaagccaa cagtcaccac gccaggaatc
    28201 gcatttatgg cgttttccat cgctatcggg tcaaggattt ccatgccgtg gacgtcgagg
    28261 atcacgttgc cgttatcggt caccacgccc tgacggtatt ccggacgacc gcccagtttc
    28321 accagctggc gcgccactgc actacgtgcc atcgggataa cttctactgg cagcgggaat
    28381 ttgcccagaa tatcaacctg cttggaagcg tcagcaatac agataaattt ttccgcaacc
    28441 gaagcaatga ttttttcacg ggtcagcgcc gcgccgccgc ctttgatcat ttgcatgtgg
    28501 ccgttgattt catctgcgcc atcaacgtag atgccaaggc tgtcgacttc gttgagatca
    28561 aaaacgtgaa tgccgaggct tttcagtttt tcagtggaag catctgaact ggaaacggcc
    28621 ccttcaatct ggcctttcat tgtaccgagc gcgtcaataa agtgtgcggc ggtggaacct
    28681 gtacctacac caacaatggt gccgggctga acatactgaa gtgccgccca tcctactgct
    28741 tttttcaatt catcctgcgt catgatcgtt tcgcctgtgg tatgaaattt cacacgcatt
    28801 atatacaaaa aaagcgattc agaccccgtt ggcaagccgc gtggttaact catccataaa
    28861 atatcgcgca atggcgggca tcccctttcg ccccgcaaat aaagcataca acggtctggg
    28921 tatgccgctc cacggtgcga acaggcgcac cagttccccc cgttcgcaag cccctgttta
    28981 caggcaaatt gaggcaataa cgccacccca ttcaacacaa ccagggcacg aactcgctga
    29041 gcgaaatgca ttagcgcaaa tccctgatct tttaagctgc gcgtttaacc acggatacaa
    29101 atgttacccg ccgacggctt cggtatatgc aacctgacac aaaattgtgt catagtgcag
    29161 gaaaaagcat ttaccaggag cagacaacag caatgaaacg cccggactac agaacattac
    29221 aggcactgga tgcggtgata cgtgaacgag gatttgagcg cgcggcacaa aagctgtgca
    29281 ttacacaatc agccgtctca cagcgcatta agcaactgga aaatatgttc gggcagccgc
    29341 tgttggtgcg taccgtaccg ccgcgcccga cggaacaagg gcaaaaactg ctggcactgc
    29401 tgcgccaggt ggagttgctg gaagaagagt ggctgggcga tgaacaaacc ggttcgactc
    29461 cgctgctgct ttcactggcg gtcaacgccg acagtctggc gacgtggttg cttccggcac
    29521 tggctcctgt gttggctgat tcgcctatcc gcctcaactt gcaggtagaa gatgaaaccc
    29581 gtactcagga acgtctgcgc cgcggcgaag tggtcggcgc ggtgagtatt caacatcagg
    29641 cgctgccgag ttgtcttgtc gataaacttg gtgcgctcga ctatctgttc gtcagctcaa
    29701 aaccctttgc cgaaaaatat ttccctaacg gcgtaacgcg ttcggcatta ctgaaagcac
    29761 ctgtggtcgc ctttgaccat cttgacgata tgcaccaggc ctttttgcag caaaacttcg
    29821 atctgcctcc aggcagcgtg ccctgccata tcgttaattc ttcagaagcg ttcgtgcaac
    29881 ttgctcgcca gggcaccacc tgctgtatga tcccgcacct gcaaatcgag aaagaactag
    29941 ccagcggtga actgattgac ttaacgccag ggctatttca acgccggatg ctctactggc
    30001 accgctttgc tcctgaaagc cgcatgatgc gtaaagtcac tgatgcgttg ctcgattatg
    30061 gtcacaaagt ccttcgtcag gattaatcca tcaaataatg cctgatagca catatcaggc
    30121 gttatcctca cttctttttg tattccttga atcacatcac aaaatagaca aatctcaggc
    30181 ggcaaaaaac gacgtctgaa tgcatttttt tgctggcgac aaacccacgt aaaaagctca
    30241 ccgtaagcgc aaataccctc attttgattg cgttttacgg agcaaataat gtctaacgag
    30301 caggagtggc aacagcttgc caacaaggaa ttgagccgtc gggagaaaac tgtcgactcg
    30361 ctggttcagc aaaccgcgga agggatcgcc atcaagcagc tgtataccga agccgatctc
    30421 gataatctgg aggtgacagg tacccttcct ggtttgccgc cctacgttcg tggcccgcgt
    30481 gccactatgt ataccgccca accgtggacc atccgtcagt atgctggttt ttcaacagca
    30541 aaagagtcca acgcttttta tcgccgtaac ctggccgccg ggcaaaaagg tctttccgtt
    30601 gcgtttgacc ttgccaccca ccgtggctac gactccgata acccacgcgt ggcgggtgac
    30661 gtcggcaaag cgggcgtcgc tatcgacacc gtggaagata tgaaagtcct gttcgaccag
    30721 atcccgctgg ataaaatgtc ggtttcgatg accatgaatg gcgcagtgct accagtactg
    30781 gcgttttata tcgtcgccgc agaagagcaa ggtgttacac ctgataaact gaccggcacc
    30841 attcaaaacg atattctcaa agagtacctc tgccgcaaca cctatattta cccaccaaaa
    30901 ccgtcaatgc gcattatcgc cgacatcatc gcctggtgtt ccggcaacat gccgcgattt
    30961 aataccatca gtatcagcgg ttaccacatg ggggaagcgg gtgccaactg cgtgcagcag
    31021 gtagcattta cgctcgccga tgggattgag tacatcaaag cagcaatctc tgccggactg
    31081 aaaattgatg acttcgctcc tcgcctgtcg ttcttcttcg gtatcggcat ggatctgttt
    31141 atgaacgtcg ccatgttgcg tgcggcacgt tatttatgga gcgaagcggt cagtggattt
    31201 ggcgcacagg atccgaaatc actggcgctg cgtacccact gccagacctc aggctggagc
    31261 ctgactgaac aggatccgta taacaacgtt atccgcacca ccattgaagc actggctgcg
    31321 acgctgggcg gtactcagtc actgcatacc aacgcctttg acgaagcgct tggtttgcct
    31381 accgatttct cagcacgcat tgcccgcaac acccagatca tcatccagga agaatcagaa
    31441 ctctgccgca ccgtcgatcc actggccgga tcctattaca ttgagtcgct gaccgatcaa
    31501 atcgtcaaac aagccagagc tattatccaa cagatcgacg aagccggtgg catggcgaaa
    31561 gcgatcgaag caggtctgcc aaaacgaatg atcgaagagg cctcagcgcg cgaacagtcg
    31621 ctgatcgacc aaggcaagcg tgtcatcgtt ggtgtcaaca agtacaaact ggatcacgaa
    31681 gacgaaaccg atgtacttga gatcgacaac gtgatggtgc gtaacgagca aattgcttcg
    31741 ctggaacgca ttcgcgccac ccgtgatgat gccgccgtaa ccgccgcgtt gaacgccctg
    31801 actcacgccg cacagcataa cgaaaacctg ctggctgccg ctgttaatgc cgctcgcgtt
    31861 cgcgccaccc tgggtgaaat ttccgatgcg ctggaagtcg ctttcgaccg ttatctggtg
    31921 ccaagccagt gtgttaccgg cgtgattgcg caaagctatc atcagtctga gaaatcggcc
    31981 tccgagttcg atgccattgt tgcgcaaacg gagcagttcc ttgccgacaa tggtcgtcgc
    32041 ccgcgcattc tgatcgctaa gatgggccag gatggacacg atcgcggcgc gaaagtgatc
    32101 gccagcgcct attccgatct cggtttcgac gtagatttaa gcccgatgtt ctctacacct
    32161 gaagagatcg cccgcctggc cgtagaaaac gacgttcacg tagtgggcgc atcctcactg
    32221 gctgccgatc ataaaacgct gatcccggaa ctggtcgaag cgctgaaaaa atggggacgc
    32281 gaagatatct gcgtggtcgc gggtggcgtc attccgccgc aggattacgc cttcctgcaa
    32341 gagcgcggcg tggcggcgat ttatggtcca ggtacaccta tgctcgacag tgtgcgcgac
    32401 gtactgaatc tgataagcca gcatcatgat taatgaagcc acgctggcag aaagtattcg
    32461 ccgcttacgt cagggtgagc gtgccacgct cgcccaggcc atgacgctgg tggaaagccg
    32521 tcacccacgt catcaggcgc taagtacaca gctgcttgac gccattatgc cgttctgtgg
    32581 taacgccctg cgactgggcg ttaccggcac acccggcgcg gggaaaagta cctttcttga
    32641 agcctttggc atgttgttga ttcgtgaggg attaaaggtt gctgttattg cggtcgatcc
    32701 cagcagcccg gtcactggcg gtagcattct cggggataaa acccgcatga atgacctggc
    32761 gcgtgccgag gcggcgttta ttcgcccggt accctcctcc ggtcatctgg gcggtgccag
    32821 tcagcgagca cgggaattaa tgctgttatg cgaagcggcg ggttatgacg tagtgattgt
    32881 cgaaacggtt ggcgtcgggc agtcggaaac ggaagtcgcc cgcatggtgg actgttttat
    32941 ctcgttgcaa attgccggtg gcggcgatga tctgcagggc attaaaaaag ggctgatgga
    33001 agtggctgat ctgctcgtta tcaacaaaga cgatggcgat aaccatacca atgtcgccat
    33061 tgcccggcat atgtacgaga gtgccctgca tattctgcga cgtaaatacg acgaatggca
    33121 gccacgggtt ctgacttgta gcgcactgga aaaacgtgga atcgatgaga tctggcacgc
    33181 catcatcgac ttcaaaaccg cgctaactgc cagtggtcgt ttacaacaag tgcggcaaca
    33241 acaatcggtg gaatggctgc gtaagcagac cgaagaagaa gtactgaatc acctgttcgc
    33301 gaatgaagat ttcgatcgct attaccgcca gacgctttta gcggtcaaaa acaatacgct
    33361 ctcaccgcgc accggcctgc ggcagctcag tgaatttatc cagacgcaat attttgatta
    33421 aaggaatttt tatgtcttat cagtatgtga acgttgccac tatcaacaaa gtggcggtca
    33481 ttgagtttaa ctatggacga aaacttaatg ccttaagtaa agtctttatt gatgatctta
    33541 tgcaggcgtt aagcgatctc aaccggccgg aaattcgctg tatcattttg cgggcgccaa
    33601 gtggatccaa agtcttctcc gcaggtcacg atattcacga actgccgtct ggcggtcgcg
    33661 atccgctctc ctatgatgat ccattgcgtc aaatcacccg catgatccaa aaattcccga
    33721 aaccgatcat ttcgatggtg gaaggtagtg tttggggtgg cgcatttgaa atgatcatga
    33781 gttccgatct gatcatcgcc gccagtacct caaccttctc aatgatgcct gtaaacctcg
    33841 gcgtcccgta taacctggtc ggcattcaca acctgacccg cgacgcgggc ttccacattg
    33901 tcaaagagct gatttttacc gcttcgccaa tcaccgccca gcgcgcgttg gctgtcggca
    33961 tcctcaacca tgttgtggaa gtggaagaac tggaagattt caccttacaa atggcgcacc
    34021 acatctctga gaaagcgccg ttagccattg ctgttatcaa agaagagctg cgtgtactgg
    34081 gcgaagcgca caccatgaac tccgatgaat ttgaacgtat tcaggggatg cgccgcgcgg
    34141 tttatgacag cgaagattac caggaaggga tgaacgcttt cctcgaaaaa cgtaaaccta
    34201 atttcgttgg tcattaatcc ctgcgaacga aggagtaaaa atggaaactc agtggacaag
    34261 gatgaccgcc gatgaagcgg cagaaattat ccagcataac gacatggtgg catttagcgg
    34321 ctttacccca gcgggttcgc cgaaagcctt accgaccgcg attgcccgca gagctaacga
    34381 acagcatgag gccaaaaagc cgtatcaaat tcgcctgctg acgggggcgt caatcagcgc
    34441 cgccgctgac gatgtacttt ctgacgccga tgctgtttcc tggcgtgcgc catatcaaac
    34501 atcgtccggt ttacgtaaaa agatcaatca gggcgcggtg agtttcgttg acctgcattt
    34561 gagcgaagtg gcgcaaatgg tcaattacgg tttcttcggc gacattgatg ttgccgtcat
    34621 cgaagcatcg gcactggcac cggatggtcg agtctggtta accagcggga tcggtaatgc
    34681 gccgacatgg ctgctgcggg cgaagaaagt gatcattgaa cttaatcact atcacgatcc
    34741 gcgcgttgca gaactggcgg atattgtgat tcctggcgca ccaccgcggc gcaatagcgt
    34801 gtcgatcttc catgcaatgg atcgcgtcgg tacccgctat gtgcaaatcg atccgaaaaa
    34861 gattgtcgcc gtcgtggaaa ccaacttgcc cgacgccggt aatatgctgg ataagcaaaa
    34921 tcccatgtgc cagcagattg ccgataacgt ggtcacgttc ttattgcagg aaatggcgca
    34981 tgggcgtatt ccgccggaat ttctgccgct gcaaagtggc gtgggcaata tcaataatgc
    35041 ggtaatggcg cgtctggggg aaaacccgga aattcctccg ttcatgatgt attcggaagt
    35101 gctacaggaa tcggtggtgc atttactgga aaccggcaaa atcagcgggg ccagcgcctc
    35161 cagcctgaca atctcggccg attccctgcg caagatttac gacaatatgg attactttgc
    35221 cagccgcatt gtgttgcgtc cgcaggagat ttccaataac ccggaaatca tccgtcgtct
    35281 gggcgtcatc gctctgaacg tcggcctgga gtttgatatt tacgggcatg caaattcaac
    35341 gcacgtagcc ggggtcgatc tgatgaacgg catcggcggc agcggtgatt ttgaacgcaa
    35401 cgcgtatctg tcgatcttta tggctccgtc gattgctaaa gaaggcaaga tctcaaccgt
    35461 cgtgccaatg tgcagccatg ttgatcacag cgaacacagc gtcaaagtga tcatcaccga
    35521 acaagggatc gccgatctgc gcggtctttc cccgcttcaa cgcgcccgca ctatcattga
    35581 taattgtgca catcctatgt atcgggatta tctgcatcgc tatctggaaa atgcgcccgg
    35641 cggacatatt caccacgatc ttagccacgt cttcgactta caccgtaatt taattgcaac
    35701 cggctcgatg ctgggttaat ggtttgtccc gtcacgatct tcagcaatat gctgaagcat
    35761 cgtggc
//
LOCUS       DAJVQG010000025        35639 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJVQG010000025 DAJVQG010000000
VERSION     DAJVQG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35639
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.025"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(234..1574)
                     /gene="fadL"
                     /locus_tag="OQM83_001125"
     CDS             complement(234..1574)
                     /gene="fadL"
                     /locus_tag="OQM83_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCS1519435.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            1946..2230
                     /locus_tag="OQM83_001126"
     CDS             1946..2230
                     /locus_tag="OQM83_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCS1519436.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            2410..3720
                     /gene="fadI"
                     /locus_tag="OQM83_001127"
     CDS             2410..3720
                     /gene="fadI"
                     /locus_tag="OQM83_001127"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCS1519437.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLCDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            3720..5864
                     /gene="fadJ"
                     /locus_tag="OQM83_001128"
     CDS             3720..5864
                     /gene="fadJ"
                     /locus_tag="OQM83_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCS1519438.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            6067..6552
                     /gene="sixA"
                     /locus_tag="OQM83_001129"
     CDS             6067..6552
                     /gene="sixA"
                     /locus_tag="OQM83_001129"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCS1519439.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            7236..7799
                     /locus_tag="OQM83_001130"
     CDS             7236..7799
                     /locus_tag="OQM83_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1519440.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            7881..10523
                     /gene="yfcU"
                     /locus_tag="OQM83_001131"
     CDS             7881..10523
                     /gene="yfcU"
                     /locus_tag="OQM83_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HCS1519441.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNIGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            10543..11294
                     /locus_tag="OQM83_001132"
                     /pseudo
     CDS             10543..11294
                     /locus_tag="OQM83_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            11269..11817
                     /locus_tag="OQM83_001133"
     CDS             11269..11817
                     /locus_tag="OQM83_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1519442.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            11814..12284
                     /locus_tag="OQM83_001134"
     CDS             11814..12284
                     /locus_tag="OQM83_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1519443.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            12281..12805
                     /locus_tag="OQM83_001135"
     CDS             12281..12805
                     /locus_tag="OQM83_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1519444.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            12792..13664
                     /locus_tag="OQM83_001136"
     CDS             12792..13664
                     /locus_tag="OQM83_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCS1519445.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(13786..14337)
                     /gene="smrB"
                     /locus_tag="OQM83_001137"
     CDS             complement(13786..14337)
                     /gene="smrB"
                     /locus_tag="OQM83_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCS1519446.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIPEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            14503..15435
                     /gene="prmB"
                     /locus_tag="OQM83_001138"
     CDS             14503..15435
                     /gene="prmB"
                     /locus_tag="OQM83_001138"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCS1519447.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            15470..16555
                     /gene="aroC"
                     /locus_tag="OQM83_001139"
     CDS             15470..16555
                     /gene="aroC"
                     /locus_tag="OQM83_001139"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCS1519448.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            16559..17383
                     /gene="mepA"
                     /locus_tag="OQM83_001140"
     CDS             16559..17383
                     /gene="mepA"
                     /locus_tag="OQM83_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCS1519449.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            17383..18192
                     /locus_tag="OQM83_001141"
     CDS             17383..18192
                     /locus_tag="OQM83_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCS1519450.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            18192..18740
                     /gene="epmC"
                     /locus_tag="OQM83_001142"
     CDS             18192..18740
                     /gene="epmC"
                     /locus_tag="OQM83_001142"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCS1519451.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            18774..19052
                     /locus_tag="OQM83_001143"
     CDS             18774..19052
                     /locus_tag="OQM83_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCS1519452.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(19173..21179)
                     /gene="mnmC"
                     /locus_tag="OQM83_001144"
     CDS             complement(19173..21179)
                     /gene="mnmC"
                     /locus_tag="OQM83_001144"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCS1519453.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            21338..22558
                     /gene="fabB"
                     /locus_tag="OQM83_001145"
     CDS             21338..22558
                     /gene="fabB"
                     /locus_tag="OQM83_001145"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCS1519454.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            22833..24011
                     /locus_tag="OQM83_001146"
     CDS             22833..24011
                     /locus_tag="OQM83_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1519455.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(24008..25003)
                     /gene="flk"
                     /locus_tag="OQM83_001147"
     CDS             complement(24008..25003)
                     /gene="flk"
                     /locus_tag="OQM83_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCS1519456.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            25102..26238
                     /gene="pdxB"
                     /locus_tag="OQM83_001148"
     CDS             25102..26238
                     /gene="pdxB"
                     /locus_tag="OQM83_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCS1519457.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            26304..27317
                     /locus_tag="OQM83_001149"
     CDS             26304..27317
                     /locus_tag="OQM83_001149"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCS1519458.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            27317..28129
                     /gene="truA"
                     /locus_tag="OQM83_001150"
     CDS             27317..28129
                     /gene="truA"
                     /locus_tag="OQM83_001150"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCS1519459.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            28212..28871
                     /locus_tag="OQM83_001151"
     CDS             28212..28871
                     /locus_tag="OQM83_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1519460.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            29027..29941
                     /gene="accD"
                     /locus_tag="OQM83_001152"
     CDS             29027..29941
                     /gene="accD"
                     /locus_tag="OQM83_001152"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCS1519461.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            30011..31279
                     /gene="folC"
                     /locus_tag="OQM83_001153"
     CDS             30011..31279
                     /gene="folC"
                     /locus_tag="OQM83_001153"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCS1519462.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            31269..31931
                     /gene="dedD"
                     /locus_tag="OQM83_001154"
     CDS             31269..31931
                     /gene="dedD"
                     /locus_tag="OQM83_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCS1519463.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            32190..32678
                     /gene="cvpA"
                     /locus_tag="OQM83_001155"
     CDS             32190..32678
                     /gene="cvpA"
                     /locus_tag="OQM83_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCS1519464.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            32715..34232
                     /gene="purF"
                     /locus_tag="OQM83_001156"
     CDS             32715..34232
                     /gene="purF"
                     /locus_tag="OQM83_001156"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCS1519465.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            34327..34896
                     /gene="ubiX"
                     /locus_tag="OQM83_001157"
     CDS             34327..34896
                     /gene="ubiX"
                     /locus_tag="OQM83_001157"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCS1519466.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            35162..>35639
                     /locus_tag="OQM83_001158"
     CDS             35162..>35639
                     /locus_tag="OQM83_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCS1519467.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDV"
BASE COUNT         8421 a         8778 c         9591 g         8849 t
ORIGIN      
        1 gtatcctgga aatgcagaaa agtaaactct ttgagaagga cgagcagaac gcctgaaaat
       61 atgcctttcc cttcaggttt gtctgtggat accgcttatt gatttgaaag ggcaagctgc
      121 caacaggctg tttgcaaaac tcaggaggta agcaatgagg acactacttt cggtgaagtg
      181 ggggagttaa agtcacctgc tatgcaggtg actttatcca ggcgaacgcg ttatcagaac
      241 gcgtagttaa agttagtacc gaacagccag gctttacctt cagactcgaa ctggtatggg
      301 ccttcgttaa ttttcacgct ctgaccgtgc atataagaaa caccaacgtc gactgaagca
      361 tctttattaa atgcgtaagt cgtacctgca ctcagccaga aacggtcctg gtccggaatg
      421 gagatagaac gattctgagc cggaactggg ctgtcatcaa aggcgatacc ggtacggaag
      481 gtccagttat catcgtagta ataagtggta cccaacgcga tgcggtaagc atctttaaag
      541 ccttcatgtt tctggaacag cgtgtcgcca ctggttgagg tcgctttcag ctgctggaac
      601 tgactccagc tggtgtaagc caggctatag tgaatcgccc actgtggatc aacacggtta
      661 taacctgaca cttcccacat ttcaggcagg ttcagcgtca gataacccga ttgcgttgcg
      721 ccacctgtcg cggtaggaat tggcaaaccg tagttattaa acgcacgatt aagatcgctg
      781 ctgtagttac ctttgaagtc aattttcact tcagaacggt aggtcagtgc atagcggtta
      841 tttttatcca gttcatacag gattccggcg ttccagccaa agccccactg gttaccgttc
      901 agatgagcga ttttggtatt actgtcgata ccgttggcgg tagctgccaa tgcttgccct
      961 tgaggagttt gcccggcagg agattgcata atctgaccag caaccaactg ccccagatcg
     1021 cctgcgaaac gttcaatttt cgcgcgagcg tagacggcgt tgaaaccaag accaaagctc
     1081 catgcattat ttaagcgata cgcaccgctt aagttcaggt tcatggtttc aaggtcagtt
     1141 gtacccccga cagagccgcc tgcataagta tcattaaact ctgttgccag accatagtta
     1201 gaggtaatag aagcgcccca accaaattgg tcgttaatcg gtgcaacaaa gtgcatgttc
     1261 ggaacccatg ccgtaggcgc gatgttatcg gctttcaggc tacgaccaga cggagacgtt
     1321 ccgctgatat tcacatccgg gtcaatataa accgcacccg cagaaaatgt cgggcggtca
     1381 aacatggtaa tcaatgcggg gttacggcta acgttacctg catcatcggc aattgcgcct
     1441 tcccctgaat aagcccggcc caggccagag gaagaaaatt cgtttaactg aaagcctgcc
     1501 gaccaggcct gggtggagat aagtgccact gcgactgcga gagcagactt tgtaaacagg
     1561 gttttctggc tcatgaccat aacctcaatg atttattttt atacaaacta tgttacgtgc
     1621 tgtaacagga gcgcgaagtg tagggtctga ggtacatctt agaaatcaga ccagtggcga
     1681 gagtataggt cggaccagct ggaatgctgc aagtatgttt ccatcttttt tcaaatatga
     1741 tctaagaaac gcgatccagc tggcaaaatt tggaggttgc gcactcgcgg attcgggtga
     1801 attttgtttt agatcatttt taagtgtgat ttcggtcact tatccgattt tgcagaatta
     1861 acaactggag cagcactttc cggaagcgta aaatatccac aacgtttatc tggcaccttg
     1921 ggtgcacata cagaggaaat caacgatgag taaatgtagt gctgatgaaa ccccggtttg
     1981 ctgctgtatg gatgttggca ccattatgga caactccgac tgcaccgcgt cttacagccg
     2041 cgtgtttgca aaccgcgcag aagcagaaca aacgctggcg gcgctgactg aaaaagcccg
     2101 tagcgtggaa tccgaaccgt gcaaaatctc cccgactttc actgaagagt ctgacggtgt
     2161 gcgcctggat atcgatttca cttttgcctg cgaagctgaa atgctgatct tccagctggg
     2221 tctgcgttaa ttcctctcgg gtacgttcgc ccctgtcagt tgctggcagg ggcgttttct
     2281 tttctttctc ctgtcttcac tgtgtcatag ctcccacttt cctttttctc cgcttggcta
     2341 aatgtaaaaa aatagttaag actatgatca ggtcagacca ctttatttat ttttttacag
     2401 gggagtgtta tgggtcaggt tttaccgctg gttacccgcc agggcgatcg tatcgccatt
     2461 gttagcggtt tacgtacgcc ttttgcccgc caggcgacgg cttttcatgg cattcccgcg
     2521 gttgatttag ggaagatggt ggtaggcgaa ctgctggcac gcagcgagat ccccgctgaa
     2581 gtgattgaac agctggtctt tggtcaggtc gtacaaatgc ctgaagcccc caacattgcg
     2641 cgtgaaattg ttctcggtac cggaatgaat gtgcataccg atgcttacag cgtcagccgc
     2701 gcttgcgcta ccagtttcca ggcggttgca aacgtcgcag aaagcctgat ggcgggaact
     2761 atccgagcgg ggattgccgg tggggcagat tcctcttccg tattgccaat tggcgtcagt
     2821 aaaaaactgg cgcgcgtgct ggttgatgtc aacaaagctc gaaccatgag ccagcgcctg
     2881 aaactcttct ctcgcctgcg tttgtgcgac ttaatgcccg taccgcctgc ggtagcagaa
     2941 tattctactg gcttgcggat gggcgacacc gccgaacaaa tggcgaaaac ctacggcatc
     3001 acccgagaac agcaagatgc attagcgcac cgttcgcatc agcgtgccgc tcaggcatgg
     3061 tcagacggaa aactcaaaga agaggtgatg actgccttta tccctcctta taaacaaccg
     3121 cttgtcgaag acaacaatat tcgcggtaat tcctcgcttg ccgattacgc aaagctgcgc
     3181 ccggcgtttg atcgcaaaca cggaacggta acggcggcaa acagtacgcc gctgaccgat
     3241 ggcgcggcag cagtgatcct gatgactgaa tcccgggcga aagaattagg gctggtgccg
     3301 ctggggtatc tgcgcagcta cgcatttact gcgattgatg tctggcagga catgttgctc
     3361 ggtccagcct ggtcaacacc gctggcgctg gaacgtgccg gtttgacgat gagcgaactg
     3421 acattgatcg atatgcacga agcctttgca gctcagacac tggcgaatat tcagttgctg
     3481 ggtagtgaac gttttgctcg tgaagtactg gggcgtgcac atgccactgg cgaagtggac
     3541 gatagcaaat ttaacgtgct tggcggttcg attgcttacg ggcatccctt cgcggcgacc
     3601 ggcgcgcgga tgattaccca gacattgcat gaacttcgcc gtcgcggcgg tggatttggt
     3661 ttagttaccg cctgtgctgc cggtgggctt ggcgcggcaa tggttctgga ggcggaataa
     3721 tggaaatggc atcagcgttt actcttaatg ttcgtctgga caacattgct atcatcacca
     3781 tcgatgtacc ggatgagaaa atgaataccc tgaaggcgga atttgcctcg caggtgcgcg
     3841 ccattattaa gcaactccgt gaaaacaaag agttgcgcgg cgtggtgttt atctccgcga
     3901 aaccggacaa ttttatcgcc ggagcagaca tcaacatgat tggcaactgc aaaacggcgc
     3961 aagaagcgga agcactggcg cggcagggcc aacagttgat ggcggagatt catgctttgc
     4021 ccattccggt tatcgcggct attcatggcg cttgcctggg cggtgggctg gagctggcgc
     4081 tggcgtgcca cggtcgcgtt tgtactgacg atcctaaaac ggtgctcggt ttgcctgaag
     4141 tacaacttgg attgttaccc ggttcaggcg gcacccagcg tttaccgcgt ctgataggcg
     4201 tcagtacagc attagagatg attctcaccg gaaaacaact tcgggcgaaa caggcattaa
     4261 agctggggct ggtggatgac gttgttccgc actccatttt gctggaagcc gctgttgagc
     4321 tggcgaagaa ggatcgccca tcttcccgcc ctctacctgt acgcgagcgt attctggcgg
     4381 ggccgttagg tcgtgcgctg ctgttcaaaa tggtcggcaa gaaaacagaa cacaaaactc
     4441 aaggcaatta tccggcgaca gaacgcatcc tggaggttgt tgaaacggga ttagcgcagg
     4501 gcaccagcag cggctatgac gctgaagctc gcgcctttgg cgaactggcg atgacgccgc
     4561 aatcgcaagc tttgcgtagt atcttttttg ccagtacgga cgtgaagaaa gatcccggta
     4621 gtgatgcacc gcctgcgcca ttaaacagcg tgggaatttt aggtggtggc ttgatgggcg
     4681 gcggtattgc ttatgtcact gcttgtaaag cggggcttcc ggtcagaatt aaagatatca
     4741 acccgcaggg cataaatcat gcgctgaagt acagttggga tcagctggag ggcaaagttc
     4801 gccgtcgtca tctcaaagcc agcgaacgtg acaaacagct ggcattaatc tccggaacga
     4861 cggactatcg cggctttgcc catcgcgatc tgattattga agcggtgttt gaaaatctcg
     4921 aattaaaaca acagatggtg gcggaagtgg agcaaaattg cgccgctcat accatttttg
     4981 cttcgaacac gtcatcttta ccgattggtg atatcgccgc tcacgccgcg cgacctgagc
     5041 aagttattgg cctgcatttc ttcagtcctg tggaaaaaat gccgctggta gagattattc
     5101 ctcatgcggg tacatcggcg caaaccatcg ccactacggt aaaactggcg aaaaaacagg
     5161 gcaaaacgcc cattgtcgtg cgtgacaaag ccgggtttta cgtcaatcgc atcttagcgc
     5221 cttacattaa tgaagctatc cgcatgctga ccgaaggtga acgggtagag cacattgatg
     5281 ccgcgctagt gaaatttggt tttccggtag gcccaatcca acttttggat gaggtaggaa
     5341 tcgacaccgg gactaaaatt attcctgtac tggaagccgc ttatggagaa cgttttagcg
     5401 cgcctgcaaa tgttgtttct tcaattttga acgacgatcg caaaggcaga aaaaatggtc
     5461 ggggtttcta tctttatggt cagaaagggc gtaaaagcaa aaaacaggtc gatcccgcca
     5521 tttacccgct cattggcgca caagggcagg ggcgactctc cgcaccgcag gttgctgaac
     5581 ggtgtgtgat gttgatgctg aatgaagcag tacgttgtgt tgatgagcag gttatccgta
     5641 gcgtgcgtga cggggatatt ggcgcggtat ttggcattgg ttttccgcca tttctcggtg
     5701 gaccgttccg ctatatcgat tctctcggcg cgggcgaagt ggttgcaata atgcaacgac
     5761 tggccacgca gtatggttcc cgttttaccc cttgcgagcg tttggtcgag atgggcgcgc
     5821 gtggggaaag tttttggaaa acaactgcaa ctgaccttca ataagaaggt caaagctata
     5881 tgaatccgcg ctgaatggcg gagtgttggt caaaatgtaa acgcatattg actatactta
     5941 cgccattgag gtaaaaaaca gcgtttcatt cggtgaatgg ataaggcaca atgccggcca
     6001 ccgttttctt tctctggttt cagatgaaag aaaacggacg aatctggtta acaaaagcgg
     6061 tgcaatatgc aagtttttat catgcgtcac ggcgacgcag cccttgatgc cgccagtgat
     6121 tcggttcgtc ctctgaccac taatggttgt gacgaatctc gcctgatggc gaactggctg
     6181 aaaggtcaaa aagtggaaat cgaacgcgtt ctggtgagcc cgttcctgcg agccgagcaa
     6241 acactggaag aagtagggga ttgtctgaac ctgccttcca gtgcagaagt tctgccggag
     6301 ttaacgccct gcggcgatgt tggtctggtc agtgcctatt tgcaggcgtt gaccaatgaa
     6361 ggtgttgctt cggtactggt gatctctcac ttaccgttag tcgggtatct ggttgccgag
     6421 ttatgtccgg gcgaaacgcc gcctatgttt accacctcgg ccattgccag cgtaacgctt
     6481 gatgaaagcg gaaacggcac attcaactgg cagatgagcc cgtgtaatct gaagatggca
     6541 aaagctatct gatgagttcc aaattatgcc caatgaggta gattcattgg gcataattgc
     6601 tcatcaggca gcggatatct gtcaatctca ctactatcga agactctccc catctataaa
     6661 taaaacactt tctactttca ttcggaaatt attcttctgc gcgtatcttg attttgccca
     6721 aagttacatg ttgaaatgaa taatgtctgt ttgacactca ttgtcattta ttaatacaga
     6781 taatagagaa aatagagttg aataatcagg caggatgtaa ttgaaacttg attaaattct
     6841 ggctatagtg tttatatatt atattgtcaa aagtggataa atgccaatcg cattatatgt
     6901 tcattgtttg ttttttaagc tgttgatatt cattattgat atactttcta ttttttctaa
     6961 atacttattc cgtatttttt aaggatgtcc ttaagataac ctttcttctg tcaatatatt
     7021 aattaaatct acatttactc tttagtaaaa ttaaattgca tcaggggtgt ggaaacatca
     7081 ctatgcggcc ccacaccaac gaatacaatt ttctacgacc tgttcttttt ggatcgcatg
     7141 ccgtattcaa tggctgggta taaacattgg ttattgactg aatcaatgta ttgcaatgtt
     7201 tatattttta attatttata gtttaaggac aaataatgag taagtttgtt aaaacagcta
     7261 ttgctgcggc aatggtgatg ggcgtgttca cctctacggc aacaatcgct gcgggtaaca
     7321 acggtactgc acgtttttac ggcaccattg aagactcagt gtgttctatc gttccggacg
     7381 atcacaaact ggaagttgat atgggtgata tcggtgccga aaaactgaaa aataacggca
     7441 ccaccacgcc gaaaagtttc cagattcgtc tgcaagattg cgtatttgat actcaggaaa
     7501 caatgaccac tacctttact ggtaccgttt cttctgcaaa tagcggcaat tattacacca
     7561 ttttcaatac cgatacctgt gcggcattta acaatgttag cctggcgatc ggtgactctc
     7621 tgggtacctc ttacaaaagc ggcatgggta ttgaccagaa aatagtgaaa gacacttcta
     7681 ccaataaagg caaagcgaag cagacactga actttaaagc ctggctggtc ggcgcagctg
     7741 atgcgccaga tctgggtaat ttcgaagcaa ataccacctt ccagattact tacctgtaag
     7801 gcataccccc atagcgacac tgctatgggg gatttaaatg gattttgacc gaacaagaat
     7861 aaaaacggat agaaacgtgt atgcctgacc attctctttt tcgattacgg attcttccgt
     7921 ggtgcattgc gctggcaatg tcagggagtt atagcagtgt ctgggctgaa gacgacattc
     7981 agtttgattc ccgttttctg gaattaaaag gcgacacaaa aattgatctg aagcgttttt
     8041 ccagccaggg atatgttgag cccggaaaat acaatttaca ggttcaacta aataaacagc
     8101 cattggcgga agagtacgat atttactggt atgctggtga agatgacgcg agcaaaagct
     8161 atgcttgtct gacaccggaa ctggtagcgc agtttggttt aaaagaagac gtggcgaaaa
     8221 atctgcaatg gagccacgat gctaaatgcc tgaaatccgg tcaactggaa ggcatggaaa
     8281 ttaaggctga tttaagccag tccgcattag tcatttcact gccacaggct tacctcgaat
     8341 atacttatcc cgactgggat ccgccttcac gttgggatga cggcatctcc gggatcgtcg
     8401 cggactacag catcaacgca caaacccggc acgaagaaaa tggcggtgat gatagtaacg
     8461 agatcagcgg caacgggacg gtcggggtta acctggggcc gtggcgtatg cgtgctgact
     8521 ggcagactaa ctatcaacat actcgcagta atgatgacga tgaattcagc ggcgatgaaa
     8581 ctcaaaaaaa atgggagtgg agtcgctact atgcctggcg ggcgttacca tcattaaaag
     8641 ccaaactggc gctgggcgag gattacctca gatccgatat ttttgatggt tttaactatg
     8701 ttggtggcag tgtcagtact gacgatcaaa tgttgcctcc caatctgcgc ggctacgcgc
     8761 cagacatttc cggcgtggca cacaccacag caaaagtgac cgtcagccag atggggcgtg
     8821 tgatttacga aacgcaggtt ccggctggac cgtttcgtat tcaggatctt ggtgattctg
     8881 tctccggtac gttgcatatt cgcattgaag aacagaacgg ccaggtgcag gaatatgaca
     8941 tcagcaccgc ctcgatgcca tacctcactc gtccaggtca ggttcgctat aagatcatga
     9001 tgggccgtcc gcaagagtgg ggacaccatg tcgagggtga atttttttct ggtgctgaag
     9061 cttcctgggg gatcgctaac ggctggtcgt tatatggcgg cgcactggga gatgaaaact
     9121 atcagtctgc ggcgcttggc gtcggtcgcg atttgtctac attcggcgcg gtcgcgtttg
     9181 atgttactca ctcgcacacc aaactggata aagacaccgc ttatggcaaa ggttcgctgg
     9241 acggtaactc cttccgtgtg agttattcca aagactttga ccagctcaac agccgcgtta
     9301 cttttgctgg atatcgcttc tcggaagaga actttatgac catgagcgag tatctggatg
     9361 ccagtgacag cggaatggta cgcacgggca acgacaaaga gatgtacact gccacttata
     9421 accagaactt ccgcgatgcg ggtgtttcgg tttatctcaa ctatacccgc catacctact
     9481 gggatcgcga ggagcagaca aactacaaca tcatgctctc gcactatttc aatataggca
     9541 gtattcgtaa tgtcagcatc tcgatgactg gctaccgtta cgagtatgac aaccaggccg
     9601 acaaaggcat gtacatttcg ctcagtatgc cgtggggtga caacagtacc gttagctata
     9661 acggtaacta tggcagtggg acggacagca gtcaggtcgg ttatttcagc cgtgtcgatg
     9721 acgcgactca ctatcagttg aacgtcggca ccagtgacaa acacaccagc gttgacggct
     9781 attacagcca tgatggttcg ctggcgcagg ttgacctcag tgcgaactac catgaagggc
     9841 aatacacctc tgcgggcttg tcgttacagg gcggcgcaac gcttactgcc cacggtggcg
     9901 cacttcaccg tacccagaat atgggcggga cacgcttgtt gattgatgcc gatggcgttg
     9961 ccgatgttcc ggtagaaggt aacggtgctg ctgtttatac caatatgttt ggtaaagccg
    10021 tcgtttctga cgtcaataac tattaccgca atcaggcgta tatcgacctc aacaaactgc
    10081 cggaaaacgc cgaagcaacc cagtcggtgg tacaagccac gctaactgaa ggggcgattg
    10141 gctaccgcaa atttgccgtc atcagtggtc aaaaagcgat ggcggtgctg cgtttacaag
    10201 acggcagcca tcctccgttt ggcgcagaag taaaaaatga taacgagcag acagtgggcc
    10261 ttgtcgatga tgacggcaat gtttatctgg ctggggtgaa acctggcgaa cacatgagtg
    10321 tgttctggag tggtgttgcg cattgcgata tcaacctgcc ggacccgctg cctgccgatc
    10381 tgtttaacgg cttgttactg ccatgccagc ataaaggcaa cgtcgcgccg gttgttcctg
    10441 atgacataaa gcctgtcatt caggagcaga cgcaacaggt gacacccacc aatccccctg
    10501 tttctgtttc agctaaccaa taacgtgatt aaggaatgat ccatgtctga tttactttgc
    10561 tctgccaaat tgggtgctac gacgctagcg ttactgctta gcgctgccag tttgtcgccc
    10621 aggcttctgt aacgccagat cgtacccgtt tgatatttaa tgaaagcgac aaatcaatca
    10681 gcgtgacgtt gcgcaataac gatccgaaac tgccttatct ggcccaaagc tggatcgaag
    10741 atgaaaaggg taacaagatc agttcaccgt taactgttct gccaccggtg caacgcatcg
    10801 attcaatgat gaacgggcag gtaaaagtgc agggaatgcc ggatatcaac aaattgcctg
    10861 ccgatcgtga aagcctgttc tacttcaacg tgcgtgagat cccgccaaaa tcaaacaaag
    10921 ccaacacgct gcaaatagca ctacaaacgc gtattaagtt gttctggcga ccgaaggcgc
    10981 tggagaacgt cagcatgaaa aacccctggc aatataaggt tactttaacc cgcaacggtc
    11041 aggaatttac cgtcaataac ccgacgccgt attacgtcat tttcagtaat gccagcacac
    11101 agaaaaatgg caatcctgca gccggtttct cgccaatagt gatgtcgccg aaaacctctg
    11161 caccgctgaa tgtgaagatg ggtagcgtgc cggtactgac ctatgtcaac gactatggcg
    11221 ctcggatgcc gctctttttc acttgcaacg gtaacagttg ccaggtcgat gaagagcaga
    11281 gccgcaaagg ctgaggccgg gagagccagt atgaaaggat cttgttctgt ctgggcggta
    11341 actgggttac tgatatgttg tgttggtagc ggctttgcag ttgcagcgag taagtcagta
    11401 agtatgaatt tacgggtgct tgtcgatgcc cctcctcctt gtacggtaaa tggtgcggca
    11461 gttgagtttg gtaatgtatt cattaataag atcaatgggg ttgattataa acgtcccatt
    11521 gactactcgc ttgtttgtaa caatctggca atggatgacc tgcgtctaca gatgcaggcg
    11581 acaacggtcg tgattaacgg tgaaacagtt atcagcacgg gtattcctgg gtttggtatt
    11641 cgggttcaga aaagtagtga tcacacaata ctcgacttaa cctctggctc atggttgcca
    11701 tttaattttt cctcgggagt tcccgttctg gaagctgtgc cggtgaaaca gagtggtaca
    11761 acgttagcgg cagctgaatt taacgccagt gccacaattg tggtggatta ccaatgaaac
    11821 ggatctcgct tattttgctg tggggctttt gctcgatggc actctcaaac gtctcttttc
    11881 acggttattt agtgcagccg cctaactgca cgattagcaa cgctcaaacc attgaaataa
    11941 cttttcagga tgtgcttatt gatgacatta atggcagcaa ttatgagcaa accgtgccgt
    12001 attccattac ctgcgatacg gcggttcgcg acccattaat ggaaatgacc ttgagctgga
    12061 gtggcacact aagcgatttt gataatgctg cagtaagcag caatatcact ggtttaggca
    12121 ttcagctcaa acaggcaggg cagagtttta cgatcaatac gccacttatg gtgaatgaaa
    12181 ccgatttacc ggtgttaacg gctgtgccag taaaaaagag tggtgtcgtt ctgccagagg
    12241 cggactttga ggcctgggcc acgttacagg tagattacca atgaagaaaa agcgtacgtt
    12301 gttttttatt agtagcctga tgttgctggg cagtggaacc acaatagcag gagataattt
    12361 gcactttacc ggcaatttaa tcagtaaatc gtgtacgcca gtgattaatg gtagccagtt
    12421 ggcagaagta cattttcctg ccattgccgc cagcgattta atgaaccttg ggcagtcaga
    12481 gcgagtgcca ttggtgtttc aattgaaaga ttgtcatagt tctacattgt ttaatgtcaa
    12541 agtaacgttg acaggaacag aagatagtgc tctacccggc tttctggcct ttgactcctc
    12601 gtcatcagca tccggcgctg ggattggtat tgaaactgcg gcaggcacat cggtgccaat
    12661 caacaataca acaggcgtca cgctcccgct gaatcaggga aataacagcc taaattttaa
    12721 tacctggtta caggcaaaga gcgggcgaga tgtaacgtcc ggagacttta gtgccacagt
    12781 gacggcaacc tttgagtatt tctgaaatga aaaaatggac cataattttc gcgagcctaa
    12841 tgctgctcgt tctgagtgtt gtcggcgcat ctaagagtta tgctggagat aaacttatga
    12901 gtgcgagttt tgacagtgtg agagtttatt atgccatgga caaggtaacc ggtgctgtag
    12961 cgagcagtgt tttcaatgtt acggtgataa cgccaaagga ggttacatat gggaaatatg
    13021 attcttttgc gtataaaggc gatacgttgc gggttatttc ctggtctggg tcgggttcag
    13081 ctccaacttt ggtactgact gactttgata caattaataa ctcaaattgc cctggcatag
    13141 atacgaaaat ttttcgttgt gcatatatga cgtttaaaat taccgttgca tctgatgatt
    13201 atggttgccc ttggatcgca tccttttatt cctatacaga tttacccgga tttggaagct
    13261 atacggcccc tacagtgcat aacacaatat gcccaacgat accggtcact tcatacgata
    13321 tctcctggag tgaaaattat gtcagccata ataaggcgtt acgaattcag tccacggggt
    13381 caacagtgac caccacgctt tcgacctacc tgatggaagg cggcagactc tgtgatggca
    13441 gtaatttttc tgataatgat ggcagaggag catactgccg cgcggtatca gaattgttaa
    13501 cctttacctc ttacggttgt gataaatcaa cggtaacggt tacgccaact cgtcatccgg
    13561 taacggataa agtactacac gatattgtgg tgaatgtgaa taccagtagt gggcaaccga
    13621 ttgattcgac gtgtcgtttt cagtatgtat tgaatgaact gtagttcgta gtattgctct
    13681 gatgcgtctt atcaggccta cgaagtgaat tgcaatttat tgaattatca catatttgta
    13741 ggccggataa ggcgtttacg ctgctccggc atttgctgcc agcattcagg gcaactccgg
    13801 cggcaaccac tcttccactt caatcaacac caacaaagcc gcatcaccgc cgtactcttt
    13861 cggtgcctgg tgaaatgcca tcacatgcgg atgttgtgcc agccacagcg gtgtttgttg
    13921 cttcaaaata tgcttcccgt gcccatgcat cacgcaggca caaaacacat gttcgcggcg
    13981 acaggcggca atcaacgcgc ccagttcctg cttggcctgc agctgcgtca gaccgtgtaa
    14041 atccaaaaac aattccggtg aataatcgcc gcggcgcagt ttcttcgcct caaaatggct
    14101 gacatccggg cgaacatatt tcaccggacc ttcggtattt aataacggct gaaactcatc
    14161 ggagaaataa tggctggcat cagcctgctc cgggatcaag cgtttcaccg gcacttcgct
    14221 gattttttta cgctgcggtc ggtggacaat cgtgtcctgc ttaatcttgc gagtccccgc
    14281 catcaactgg cgaaacagag cctggtcctc ctcgctaagt gttgttttct ttttcattta
    14341 tctgtctcta tttctaattt tcctacagtt tacccgactc gtagcacaat cgatcgtttt
    14401 aaacgtattt ttcctcactc accgcgcgtg aatgctgatt tatcgtcatc ttcatggcaa
    14461 actagccgcc gaaattattg cgatcatgcc ctggaggaat acgtggataa aattttcgtt
    14521 gatgaagcag taaatgagct gcaaaccatt caggacatgt tgcgctggtc ggtaagccgc
    14581 ttcagcgcgg caaatatctg gtacggtcac ggcaccgata acccgtggga tgaagccgta
    14641 cagctggtgt taccatcgct ctacctgccg ctggatatcc cggaagatat gcgcaccgcg
    14701 cgtctgacct ccagcgaaaa acaccgtatt gttgaacgcg tgatccgccg cgtcaacgag
    14761 cgcattccgg tggcttacct gaccaacaaa gcctggttct gcggtcatga attttacgtc
    14821 gatgaacgcg tgctggtgcc gcgctcgccg attggtgaac tgatcaacaa taaatttgcc
    14881 ggactcatta gcaagcaacc gcagcatatt ttagatatgt gtaccggtag cggctgcatc
    14941 gccattgcct gtgcttatgc cttcccggaa gcggaagtcg acgcggtaga tatctctccc
    15001 gacgcgctgg cggttgccga acagaacatc gaagaacacg gtctgatcca caacgtcatt
    15061 ccgattcgtt ccgatctgtt ccgtgatttg ccgaaagtgc agtacgatct gattgtcact
    15121 aacccgccgt atgtcgacgc ggaagatatg tccgacctgc caaacgaata ccgtcacgag
    15181 ccggaactgg gcctggcatc tggcaccgac ggccttaaac tgacgcgtcg cattcttgga
    15241 aacgcggcag attaccttgc tgatgatggc gtgttgattt gtgaagtcgg caacagcatg
    15301 gtacatctta tggaacaata tccggatgtt ccgttcacct ggctggagtt tgataacggc
    15361 ggcgatggtg tgtttatgct caccaaagag cagctgctgg cggcccgtga atatttcgcg
    15421 atttataaag attaagtaaa cacgcaaaca caacaataac ggagccgtga tggctggaaa
    15481 cacaattgga caactctttc gcgtaaccac cttcggcgaa tcgcatgggc tggcgctcgg
    15541 ctgcatcgtc gatggtgttc cgccaggcat tccgctgacg gaagcggacc tgcaacatga
    15601 cctcgaccgt cgtcgccctg ggacatcgcg ctataccacc cagcgccgcg agccggatca
    15661 ggtcaaaatt ctctccggtg tttttgaagg cgttactact ggcaccagca ttggcttgtt
    15721 gatcgaaaac actgaccagc gctctcagga ttacagcgca attaaagacg ttttccgccc
    15781 aggccatgct gattacacct acgaacaaaa atacggtctg cgcgattatc gcggcggtgg
    15841 acgttcttcc gcccgcgaaa ccgccatgcg cgtggcggca ggggcgattg ccaaaaaata
    15901 tctcgccgag aaatttggta ttgaaatccg cggctgcttg acccagatgg gcgacattcc
    15961 gctggaaatc aaagactggt cgctggttga acaaaatccg ttcttctgcc cggacccgga
    16021 caaaatcgac gcgttagatg aactgatgcg cgcgctgaaa aaagagggcg actccatcgg
    16081 cgcgaaagtc accgttgttg ccagtggtgt ccccgccgga cttggcgagc cggtctttga
    16141 ccgcctggat gccgacatcg cccatgcgct gatgagcatc aacgcggtga aaggcgtgga
    16201 aattggcgac ggttttgacg tagtggcgct gcgcggcagc cagaaccgcg acgaaatcac
    16261 caaagacggt ttccagagca accatgcggg cggcattctc ggcggtatca gcagcgggca
    16321 gcaaatcatt gctcatatgg cgctgaaacc gacctccagc attaccgtgc cggggcgcac
    16381 tattaaccgc tttggcgaag aagttgagat gatcaccaaa ggccgtcacg atccctgtgt
    16441 cgggatccgt gcagtgccga tcgctgaagc gatgctggcg atcgttttaa tggatcacct
    16501 gttacggcag cgggcgcaaa atgccgatgt gaagactgat attccacgct ggtaaaaaat
    16561 gaacaaaacc gcgattgcgc tgctggctct gcttgccagt agcgctagcc tggcagcgac
    16621 gccgtggcaa aaaataaccc aacctgtgcc gggtagcgca caatcgatag gcagtttttc
    16681 taatggctgt attgtcggcg ctgacacgtt gccgatacag tccgaacatt atcaggtcat
    16741 gcgtaccgat cagcgtcgct atttcggtca cccggatctg gtgatgttta tccagcgtct
    16801 gagtagccag gtgagcaatc tgggcatggg tacggtgctg attggcgata tggggatgcc
    16861 cgctggtggg cgtttcaacg gcggtcatgc cagccaccag accggactgg atgtcgatat
    16921 ctttctgcaa ctgccgaaaa ctcgctggac ctccgcgcag ctcttgcgcc cgcaagcact
    16981 ggacttagtt tcccgcgacg gtaaacacgt tgtctccacg ctgtggaagc cagaaatttt
    17041 cagcttgatc aaactcgctg cccaggacaa agacgtcacg cgcatttttg ttaatccggc
    17101 gattaaacaa caactttgcc ttgatgcggg caccgatcgc gactggttgc gcaaagtgcg
    17161 accctggttc cagcatcgcg cgcatatgca tgtacgatta cgttgccctg ccgatagtct
    17221 ggagtgtgaa gatcaacctt taccgccatc aggcgatggt tgcggggcag aactgcaaag
    17281 ctggtttgca cctctaaaac cgggaacaac aaagcctgag aagaagacac cgcctccgtt
    17341 gccgccttcc tgccaggcgc tactggatga gcacgtgatc taatggaaac gtttaatagc
    17401 ctgtttatgg tttccccgct gttgctggga gttctctttt ttgtcgccat gctggcggga
    17461 tttatcgact cgattgccgg tggcggtggg ttactcacca ttccggcatt gatggctgcg
    17521 ggaatgtctc ccgctaacgc gctggcaacc aataaactgc aagcctgcgg cggctctatt
    17581 tccgccacta tctactttat tcgccgcaaa gtggttagtt taagcgatca gaaactcaat
    17641 attgccatga cctttgtcgg ctcaatgagc ggcgcactgt tggtacagta cgttcaggct
    17701 gatgtcttac ggcagatttt gcccattctg gtgatttgta tcggcctcta tttcttgctg
    17761 atgccaaagc tgggtgaaga agatcgccag cgccggatgt acggtttacc cttcgcgtta
    17821 attgccggtg gctgcgtcgg tttttatgac ggattctttg gcccggctgc cggatcgttt
    17881 tacgcgctgg ccttcgttac gctgtgcgga ttcaacctcg ccaaagccac ggctcacgcc
    17941 aaattactca acgcaacgtc aaacattggc ggtctgctgc tgtttattct cggcggcaaa
    18001 gtgatttggg cgacgggctt tgtcatgctg gtggggcagt tcctcggcgc gcgcatgggt
    18061 tcacggctgg tgttgagcaa aggacaaaag ctgatccgcc cgatgattgt tatcgtctcg
    18121 gcggtgatga gtgccaaatt actttacgat agccacggac aggagatcct ccactggttg
    18181 gggatgaact aatgaacagt acacaccact atgagcagtt gattgaaatt tttaatagct
    18241 gctttgccga tgaatttaat acccgtctga ttaaaggcga cgacgaaccg atctatcttc
    18301 ctgctgatgc ggaagtaccg tataaccgaa ttgtctttgc ccacggcttt tatgccagcg
    18361 caattcatga gatttcgcac tggtgtattg ccggaaaagc gcgccgtgag ctggttgatt
    18421 tcggctactg gtattgcccg gatgggcgtg atgcccaaac gcaaagccag tttgaagatg
    18481 ttgaagtgaa gccgcaggca ctcgactggt tgttctgtgt ggcggcggga tatccgttta
    18541 acgtcagttg cgacaatctg gaaggcgact tcgaaccgga tcgtgttgtg tttcaacggc
    18601 gggtacatgc gcaggtgatg gattatctga caaatggcat tcccgagcgt ccggcacgct
    18661 tcattaaagc actacaaaat tattatcaca cgccggaact tacggcggaa cagttcccgt
    18721 ggccggaagc gctcaactga agccggtgcc acgttttaca atgaggaaag aagatgatcg
    18781 cggagtttga atcacgcatt ctggcattaa tcgacggcat ggttgaccat gccagtgatg
    18841 atgagttgtt tgccagtggg tatttgcgtg gccacctgac attagccatc gcagaactgg
    18901 aaagtggtga tgaccactcc gctcaggcgg tgcatacgac cgttagccag agtctggaaa
    18961 aagccattgg tgccggtgaa ttgtcgccgc gtgaccaggc gctggtgacc gatatgtggg
    19021 aaaacctgtt tcagcaggcg tcacagcagt aagttgcggc gtctggagca ccatgcctga
    19081 tgcgacgctt atcgcgtctt atcaggccta cacgtcacac gtaggtcgga taaggcgttc
    19141 acgccgcatc cggcaaacac tgcgagagca gattaccccg ccttaaccgc tttacccttc
    19201 aacaatttcc gcacccataa ccgattcggg tttaacgccg tcagcgtact ggcatccatc
    19261 ggaatcggtt cgtcgctcat ctgcgccgcc agaatctcgg cacacagcgg ggcagaacac
    19321 aaaccgcgag aacctaaagc cgcaaacata aagagatcgt caaaaaccgg cgcgcttacc
    19381 gcctcatctt tctgctccgc caacgacgca tattccacga gtgttgcctc ataatcggga
    19441 acattgccta ccattggcag atgatcgcgg gtggcacaac gcacaccgca gcgcgcctct
    19501 ttatcactga catcaacctc ttttgcccac tgtgcctgcg ggaaacaatc aatcaaccgc
    19561 tggcgattct gctgctgatc gtcctcactg tacgccgtat cttcgctgcc gcgatgataa
    19621 ctggcaccaa tacaatgatg ttgattcgcc ggattttgtg gcgtgagata accgtcatag
    19681 cacagcacct gcttcagctc tgccagttcc ggcgttgtcg gaatatggct gacctgcccg
    19741 gcaaccgaat acaccgggag agtcgacgtt tggctgaaac ggctgatttg atgcccgttc
    19801 gccagtacca ctacgctgtg tgttgcttgc tgatctcctg caaaattcaa caaccaacag
    19861 tcatccttac gggataaatt ctgtaactga tattgataat aaatctgcaa accctgctgt
    19921 tgcgccagtt ccagcacatt acgggtcagt tctgctgggc acagccaacc accttgcgga
    19981 taagtaatgc cgctgcaatt tgtcgcaacg cccgtaattt gttcaaccgc atttgcctca
    20041 acggctacag ccagttctgc gggtaaatcc attgacaaca tctgtgcgat tttatgctgg
    20101 cttttctcat cccagcctaa ctgcgtgacg ccgcaccagt catgatcaaa tttaacgggt
    20161 aattggtcgt aaaaccgacg agcaaaagta aacgcattag agaaaaagcg gtttagcgcc
    20221 tcatcgtgtt tgcttaataa cggatacagc gccccctggc gattgccgga agcacccagc
    20281 gcgggggctt catccgcgca ataaagcgct acctgccagc cgcgccgtaa taacgccagc
    20341 gacaacaacg cgctggcaat accaccgccg ataatcgcta cttcccgttt gctgctgccc
    20401 gtacgattaa accacggcgt ggagcagggg agtggtaatg tctgttccat caccccacaa
    20461 agcatttccc gtttgcgccc aaagccctta cgtttttgca tcgtgaatcc ggcttcctgc
    20521 aaaccgcggc ggacaaaacc ggcagacgta aatgtcgcca gcgtgccgcc tggacgcgcc
    20581 aaccttgcca tggcgttaaa cagattttgc gtccacatat ccgggttttt agctggcgca
    20641 aagccgtcca gaaaccaggc atctactttt tgatttagcg aatcgtccag ttggctggtc
    20701 agttcgttaa tatcgccaaa ccataaatcc agcgtcacgc ggccttcatt gagcaataaa
    20761 cgatggcaac cgggcaaggg cattggccac tgcgcctgaa gttgttctgc ccacggagcc
    20821 agttccggcc agtgttgatg cgctaaggct aaatccgcac gggtgagggg aaatttctca
    20881 aaactaatga aatgtaagcg ttgtaattgc gcttgcggat gcgcttcgcg aaactgatca
    20941 aatgcctgcc atagcgtcag gaagtttaat ccggtgccga agccgctctc tgctaccaca
    21001 aacagaggat gtggatgctc aggaaagcgt acctctaatt ggttgcctcc cagaaaaaca
    21061 taacgcgtct cttccagccc gttatcgttg gaaaaataga catcgtcaaa atctcgggaa
    21121 acaggtgtac cctcagcatt aaattcgagg ttggcaggtt gtatggagta gtgtttcacg
    21181 taagttactc gtcttacagg cggtggctcg atcttagcga tgtgtgtaag gctgcgcaaa
    21241 tttctctatt aaatggctga tcggacttgt tcggcgtaca agtgtacgct attgtgcatt
    21301 cgaaacttac tctatgtgcg acttacagag gtattgaatg aaacgtgcag tgattactgg
    21361 cctgggcatt gtttccagca tcggtaataa ccagcaggaa gtcctggcat ctctgcgtga
    21421 aggacgttca gggatcactt tctctcagga gctgaaggat tccggcatgc gtagccacgt
    21481 ctggggcaac gtaaaactgg ataccactgg cctcattgac cgcaaagttg tgcgctttat
    21541 gagcgacgca tccatttatg cattcctttc tatggagcag gcaatcgcag atgcgggcct
    21601 ttctccggaa gcttaccaga ataacccgcg cgttggcctg attgcaggtt ctggcggcgg
    21661 ttccccgcgt ttccaggtat tcggcgctga cgcaatgcgc ggcccgcgcg gcctgaaagc
    21721 ggttggcccg tatgtggtca ccaaagcgat ggcatccggc gtttctgcct gcctcgccac
    21781 gccgtttaaa attcatggcg ttaactactc catcagctcc gcgtgtgcga cttccgcaca
    21841 ctgtatcggt aacgcagtag agcagatcca actgggcaaa caggacatcg tgtttgctgg
    21901 cggcggcgaa gagctgtgct gggaaatggc ctgcgaattc gacgcgatgg gtgcgctgtc
    21961 tactaaatac aacgacaccc cggaaaaagc ctcccgtact tacgacgctc accgtgacgg
    22021 tttcgttatc gctggcggcg gcggtatggt agtggttgaa gagctggaac acgcgctggc
    22081 gcgtggtgct cacatctatg ctgaaatcgt tggctacggc gcaacctctg atggtgcaga
    22141 catggttgct ccgtctggcg aaggcgcagt acgttgtatg cagatggcga tgcatggcgt
    22201 tgatacccca atcgattacc tgaactctca cggtacttcg actccggttg gcgacgtgaa
    22261 agagctggca gctatccgtg aagtgttcgg cgataagagc ccggcgattt ctgcaaccaa
    22321 agccatgacc ggtcactcac tgggcgctgc gggtgtgcag gaagctatct actctctgct
    22381 gatgctggaa cacggcttta tcgccccgag catcaacatt gaagagctgg acgagcaggc
    22441 tgcgggtctg aacatcgtga ccgaaacgac cgatcgcgaa ctgaccactg ttatgtctaa
    22501 cagcttcggc ttcggcggca ccaacgccac gctggtaatg cgcaagctga aagattaatt
    22561 cgcagtaggc cggagtaggt cggataagac gcgccagcgt cgcatccgac gttacgcgcc
    22621 aatgcggcct ccggcactaa cgcaaaaggg aacctgatgg ttcccttttt cacatcattg
    22681 acaatcgcca ccagttccag gcaaacttcc cgctttgtcg atttccttct gaaaagacgt
    22741 acgcgttaaa tcctgccaac gcaccgtaac cctgaaacca gagagatgag acggggatac
    22801 tcctcgcctt gcgctgcatt ctggagtaat gcatgactgc tgtaagccaa accgaaacac
    22861 gatcttctgc caatttttcg ctcttccgca tcgcttttgc ggtttttctc acctacatga
    22921 ccgtagggct gccgttgccg gttatcccgc tgtttgttca tcatgaactg ggctatggca
    22981 ataccatggt cggcattgcc gtcgggattc agtttctggc tacggtgctg acgcgtggct
    23041 atgccgggcg actggccgat caatatggcg caaaacgttc ggcgcttcag gggatgttag
    23101 cttgtggtct ggctggcggc gcgttgctgc tggcggcgat tttgcctgtc tccgcaccgt
    23161 tcaaatttgc cctgttggtc atcgggcgtt tgattcttgg gtttggtgaa agccagttac
    23221 tgacaggcgc tctgacctgg gggttaggca tcgtagggcc aaaacactct ggcaaagtga
    23281 tgtcatggaa tggaatggcg atttacggtg ccctcgctgt tggtgctccg cttggcctgt
    23341 tgattcatag ccattacggt tttgccgcac tggcgatcac cacaatggta ttacccttac
    23401 tggcgtgggc ctgtaacggc acagtgcgca aagtaccggc cctggcggga gaacgtccat
    23461 cgctgtggag cgttgtcggg tttatctgga aaccagggtt agggctggca ctacaaggcg
    23521 ttggttttgc ggttatcggg actttcgttt cgctctactt tgccagcaaa ggatgggcga
    23581 tggcgggctt tactcttacc gagtttggcg gcgcatttgt cgtgatgcgc gtcatgtttg
    23641 gctggatgcc ggaccgtttt ggcggcgtga aagtggcgat tgtctctctg cttgtagaaa
    23701 cggtgggctt gttgctgctc tggcaagccc caggtgcgtg ggtcgcatta gcgggcgcgg
    23761 cgttaaccgg agccggatgt tcgcttatct ttcctgcgct gggcgtggag gtggttaaac
    23821 gcgtcccctc acaagttcgc ggcaccgcac tgggcggtta cgccgcgttt caggatatcg
    23881 ccctcggcgt ctccgggccg ctggcgggaa tgctggcgac cacgtttggt tactcttcgg
    23941 tatttcttgc cggggcgatc tctgcggtgc tgggaattat tgtcacgata ctgtcatttc
    24001 gtcggggcta acgaaccagc cagaccagca tcaggataat cgcgactaac agtatccaca
    24061 gcgcgggacg cgttgccata ttttgcaacg tgtccatcat cggtggaaaa ggattaagca
    24121 gcggttgcat atcacgcgga tcaatccctt taacccgccg ttgataaagc tgattaagaa
    24181 tatcctcggc ctgggcagaa gaaagcgatg attgcgcaga aagaccatat ttttgctgga
    24241 tatacgccga taacgctgcc agttcactgg catctaaagg ttgttttagc gccatctgta
    24301 gtgattccag cgtcggcgta ttttgctggc ttaacgtctg gcgcgcctgc aaccaggtca
    24361 ccagatggtt aaacagtttc gctggaatta actcgccatc gttcaccccg gaaagttcca
    24421 gcatcgattg ccagatctgt ttgctgggtt cccccgttgc cgccgcaagt ttggtcacca
    24481 gctgttttag cgcattgtgc tccgccggta ataaaggacg gtcggtcgcc tcgcgctgct
    24541 gtggttgcgg aataaccatc ttcccttcct gcaacagggt gagaatggtt tttaattgct
    24601 ccggtgagag ctgattcagc ggcgtctgac caaagttatg acggatataa tccgtgaccg
    24661 cctgacgatt attccccaga cgtaaatact cccctaattg cgctaaaagc tggcgggcag
    24721 aatggctttt ttgcgcggcc agcagacgtt gcgccagatt atgctcagcg gcagggaagt
    24781 gacgcgaaag cagcggtgaa tctcccgaca gaccaatatc gtgcctgata cccgcccaca
    24841 gttctgctct ttgttgttgc gtcagtgagg tcactttcgt cattaagctt tccagcgaag
    24901 tacgttgctg actggataaa ggctgattgc ccgcgccaga cggcaggtta tctccctgac
    24961 ctggtggttg cccaggagga gggccggaaa taggttgtat cattacgtat ccttatacct
    25021 gaaatcttcg caagtatgcc tggccgcgag attatggcac acttgtccgg ttaactctcg
    25081 tctcatacag gtaacacaaa cgtgaaaatc cttgttgatg aaaatatgcc ttatgcccgc
    25141 gacttattta gccgtttggg tgaggtgacc gcggttcccg ggcgtccaat ccccgtcgct
    25201 caactggcag acgcggatgc gctgatggtg cgttcggtca cgaaagtgaa tgaatctttg
    25261 ctggcaggaa aacccattaa atttgttggc actgccactg cggggaccga ccatgtcgat
    25321 gaagcatggt tgaagcaggc gggaattggt ttttccgctg cacctggctg taatgcgatt
    25381 gcggtggtgg aatatgtttt ctcctcacta ctgatgcttg ccgaacgcga tggattttca
    25441 ctgcacgacc gtacggtggg gatcgtgggc gttggtaacg ttggacgtcg attgcaggcg
    25501 cgactggaag cgttagggat taaaacattg ctttgcgatc cgcctcgcgc tgaccgtggg
    25561 gatgagggtg atttccgctc gctggatgaa ttagttcagc atgcggatat tctgactttc
    25621 catacgccac tcttcaaaga tgggccgtac aaaacgctgc atctggcgga tgaaaaactg
    25681 atccgtagcc tgaagccggg agcgattctg attaacgcct gccgtggcgc agtcgtcgat
    25741 aatactgcgt tgctgacctg cctgaatgaa ggccagaagt taagcgtagt gctggatgtc
    25801 tgggaaggcg aaccggaact taacgtggag ttgctgacaa aagtggatat cggcacgccg
    25861 catatcgcag gttataccct ggaaggtaaa gcacgcggta ctacgcaagt gtttgaagcc
    25921 tatagcaagt ttattgggca tgaacagcac gttgcgctgg atacattact gcccgcgcca
    25981 gagtttggtc gcattacgct gcatggcccg ctcgatcaac cgacgctgaa aaggctggtg
    26041 catttggtgt atgatgtgcg ccgcgatgac gcaccgctgc gtaaagtcgc cgggataccg
    26101 ggtgagttcg ataaactgcg caaaaactat cttgagcgcc gtgaatggtc atctctgtat
    26161 gtaatttgtg atgacgccag tgcggcatca ttgctgtgta aactgggttt taacgccgtt
    26221 catcatccgg cacgttaatc tcttcttcat gctctctgct gtaacattgg cagggagctt
    26281 tgctatttct ggagtaaacc accatgtctg aaggctggaa cattgccgtc ctgggcgcaa
    26341 ctggcgctgt gggcgaagcc ctgcttgaaa cgctggctga acgtcagttc ccggttgggg
    26401 aaatttatgc actggcacgt aacgaaagcg caggcgaaca actgcgcttt ggtggtaaaa
    26461 caatcactgt gcaggatgcc gctgaattcg actggacgca ggcgcagctg gcattttttg
    26521 tcgcaggcaa agaagctacc gctgcctggg ttgaagaagc gaccaactca ggttgcctgg
    26581 tgatcgacag cagcggactg tttgctctcg aacccgacgt accgctggtg gtgccggaag
    26641 taaacccgtt tgtactgacc gattaccgca accggaatgt catcgccgta ccagacagtc
    26701 tgaccagcca gctgctggcg gcactgaaac cgttaatcga tcagggcggt ttgtcgcgta
    26761 tcagtgttac cagcctgatt tcagcctccg cccagggcaa aaaagcggtc gatgcgttag
    26821 cggggcagag tgcgaaattg ctcaacggca ttccgattga cgaagaagat ttcttcggtc
    26881 gtcaactggc gttcaatatg ctgccgttgt tgccggatag cgaaggtagc gtgcgtgaag
    26941 aacgtcgtat cgttgacgaa gtacgcaaaa tcctgcagga cgaagggctg atgatttcgg
    27001 caagcgtcgt ccaggcaccg gtattctacg gtcatgccca gatggtcaac tttgaagcac
    27061 tgcgtccgct ggcggcagaa gaagcgcgtg atgcgtttgc tcagggcgaa gatattgtgc
    27121 tctctgaaga gaacgaattc ccgactcagg taggggatgc ttcgggtacg ccgcatcttt
    27181 ctgttggctg cgtgcgtaat gactacggta tgccggagca agtccagttc tggtcggtgg
    27241 ccgataacgt tcgctttggc ggcgcgctga tggcagtaaa aatcgccgag aaactggtgc
    27301 aggagtatct gtactaatgt ccgaccagca acaactgcca gtttataaaa ttgcgctggg
    27361 cattgagtac gacggcagta agtattacgg ctggcaacgg cagaatgaag tccgcagtgt
    27421 gcaggagaag ctggaaaagg cgctctccca ggtggcgaac gaacccatca ccgtcttctg
    27481 cgccgggcgt actgacgcag gggtacacgg taccgggcag gtggtgcatt tcgaaacaac
    27541 cgcgcagcgc aaagacgcgg cgtggacctt gggcgtaaat gcgaatttac ctggtgacat
    27601 cgccgtgcgt tgggttaaag ctgtacctga tgattttcat gcccgattta gcgccacggc
    27661 tcgccgttat cgctacatca tctacaatca tcggctgcgc ccggcggtat tgagtaaagg
    27721 ggtaacccat ttttacgaac cgctggacgc tgaacggatg catcgggctg cgcaatgctt
    27781 gctgggcgag aatgatttca cctcgttccg tgcggtgcag tgccagtccc gaaccccgtg
    27841 gcgcaacgtt atgcatatta atgtcacgcg tcacggtcct tatgtggtgg tagatatcaa
    27901 agcgaatgcc tttgtacatc atatggtcag gaatattgtc ggcagcctga tggaagtcgg
    27961 tgcccacaac cagccggaga gatggatagc agagttgctg gcggcaaagg acagaacgct
    28021 ggcggcagca acggcaaaag cggaagggct gtatctggtc gcggtggatt accctgaccg
    28081 gtatgatctt ccaaaaccgc caatgggccc gctatttctg gcggactaac gagaataatg
    28141 ctcggaacat ttcaggctgt agccaacgta gttacagcct gaaagatgac gagtacaagg
    28201 cataggcaaa tatggacctg atttatttcc tcatcgattt tatcctgcac attgacgtgc
    28261 atctggcaga gctggtcgcg gagtacggcg tctgggttta tgccattctg tttttgattc
    28321 tgttctgcga aacggggctg gtggtaacgc cattcctgcc tggtgattcg ctgctgtttg
    28381 ttgctggtgc attggcgtcg ctggaaacta acgatctcaa tgtccatatg atggtggtgc
    28441 ttatgttgat tgccgctatt gtcggtgatg cagtgaacta taccattgga cggttatttg
    28501 gtgacaaact gttcagtaac ccggattcga aaatctttcg tcgcagttat cttgataaga
    28561 ctcatcagtt ttacgagaga catggcggca aaacgattat tctcgcccgt tttgtaccga
    28621 tcgtcagaac gttcgcgccg tttgttgcgg gaatgggcca catgtcgtac cgtcatttcg
    28681 ccgcttataa cgtgatcggc gcactgttgt gggtactgat ttttacctac gcgggttatt
    28741 tcttcggcac gctccccttc attcagagca accttaagct gatgattgtt ggcattattt
    28801 ttgtttcaat attgcctggg gttttcgaat ttattcgtca caaacgcgct gcggcacgcg
    28861 ccgcaaaata aaaagtaact ccgcggttcg accacttttt tatccaaagt ttcgggctgt
    28921 tatgttttaa tgtgcaacat tcatggtctg ttgggggcaa aaatggcatt atgcgtcccc
    28981 aaagataaaa ctggcatcga accaggttca gacagaaagg tccctaatga gctggattga
    29041 acgaattaaa agcaacatta ctcccacccg caaggcgagc attcctgaag gggtgtggac
    29101 taagtgtgat agctgcggtc aggttttata ccgcgctgag ctggaacgta atcttgaggt
    29161 ctgtccgaag tgtgaccatc acatgcgtat gacagcgcgt aatcgcctgc atagcctgtt
    29221 agatgaagga agccttgtgg agctgggtag cgagcttgag ccgaaagatg tgctgaagtt
    29281 tcgtgactcc aagaagtata aagaccgtct ggcttctgcg cagaaagaaa ccggcgaaaa
    29341 agatgcgctg gtggtgatga aaggcactct gtatggaatg ccggttgtcg ctgcggcatt
    29401 cgagttcgcc tttatgggcg gttcaatggg gtctgttgtg ggtgcacgtt tcgtgcgtgc
    29461 cgttgagcag gcgctggaag ataactgccc gctgatctgc ttctccgcct ctggtggcgc
    29521 acgtatgcag gaagcactga tgtcgctgat gcagatggcg aaaacctctg cggcactggc
    29581 aaaaatgcag gagcgcggct tgccgtacat ctccgtgctg accgacccga cgatgggcgg
    29641 tgtttctgca agtttcgcca tgctgggcga tctcaacatc gctgaaccga aagcgttaat
    29701 tggctttgcc ggtccgcgtg ttatcgaaca gaccgttcgc gaaaaactgc cgccaggatt
    29761 ccagcgcagt gaattcctga tcgagaaagg cgccatcgac atgatcgtcc atcgtccgga
    29821 aatgcgcctg aaactggcga gcattctggc gaagttgatg aatctgccag cgccgaatcc
    29881 tgaagcgccg cgtgaaggcg tagtggtacc cccggtaccg gatcaggaac ctgaggcctg
    29941 ataactgata agggcagggc cactggctct gcccttttgc tattctcacc gtaacgaatc
    30001 agcggatacc atgattatca aacgcactcc tcaagccgcg tcgcctctgg cttcgtggct
    30061 ttcttatctg gaaaacctgc acagtaaaac tatcgatctc ggccttgagc gcgtgagcca
    30121 ggtcgcggcg cgtcttggcg tcctgaaacc agcgccattt gtgtttaccg ttgcgggtac
    30181 gaatggcaaa ggcaccacct gccgtacgct ggagtcgatt ctgatggcgg cagggtacaa
    30241 agtgggcgtc tacagttcac cgcatctggt gcgttatacc gagcgcgtac gtgtgcaggg
    30301 gcaggaattg ccggaatcgg cccacaccgc ctcttttgcg gagattgaat cggcacgcgg
    30361 tgatatttcc ctgacctatt tcgagtacgg tacgctgtcg gcgttgtggc tgttcaaaca
    30421 ggcacaactt gacgtagtga ttctggaagt agggctgggc ggtcgtctgg acgcaaccaa
    30481 tattgtcgat gccgatgtgg ctgtagtaac cagcattgcg ctggatcata ccgactggct
    30541 gggaccagat cgcgaaagta ttggtcgcga gaaagcaggc atctttcgca gcgcaaaacc
    30601 ggcaattgtc ggtgagccgg aaatgccttc taccattgct gatgtggcgc aggaaaaagg
    30661 tgcactgtta caacgtcggg gcgttgagtg gaactattcc gtcaccgatc atgactgggc
    30721 gtttagcgat gctcacggca cgctggaaaa tctgccgttg ccgcttgtcc cgcaaccgaa
    30781 tgccgcaaca gcgctggcgg cactgcgtgc cagcgggctg gaagtcagtg aaaatgccat
    30841 tcgcgacggg attgccagcg caattttgcc gggacgtttc cagattgtga gcgagtcgcc
    30901 acgcgttatt tttgatgtcg cgcataatcc acatgcggcg gaatatctca ccgggcgtat
    30961 gaaagcgcta ccgaaaaacg ggcgcgtgct ggcggttatc ggtatgctac atgataaaga
    31021 tattgccgga actctggcct ggttgaaaag cgtggttgat gactggtatt gtgcgccact
    31081 ggaagggccg cgcggtgcca cggcagaaca actgcttgag catttgggta acggcaaatc
    31141 atttgatagc gttgcgcagg catgggatgc cgcaatggcg gacgctaaag cggaagacac
    31201 cgtgctggtg tgtggttctt tccacacggt cgcacatgtc atggaagtga ttgacgcgag
    31261 gagaagcggt ggcaagtaag tttcagaatc ggttagtggg cacgatcgtg ctggtggcgc
    31321 tgggggtgat tgtacttcca gggctgctgg acgggcagaa aaaacattat caggatgagt
    31381 ttgcggctat cccgctggtt ccaaaagcgg gcgatcgcga tgagcctgat atgatgccag
    31441 ccgccaccca ggcgttgccg acgcagccgc cggaaggcgc agcggaagag gtgcgggcag
    31501 gtgatgccgc agcaccgtcg ctcgatccgg ccactattgc agccaataac accgagtttg
    31561 aaccggaacc tgcaccggtc gccccaccga agccgaaacc ggtggagccg cctaaaccga
    31621 aggttgaagc accacctgcg ccgaagccag aaccgaagcc ggtcgtggaa gaaaaagcag
    31681 cgccaacggg taaagcctat gttgtgcaac tgggtgcgct gaaaaatgcc gataaagtga
    31741 atgagattgt cggtaagctg cgcggtgccg gttatcgggt ttatacgtcg ccatccacgc
    31801 cagtgcaggg taaaattacc cgtattctgg ttgggccgga tgcctcgaaa gataagctga
    31861 aaggttcgct gggtgagttg aagcaacttt ctggcttaag tggcgtggta atgggctata
    31921 cgccgaatta atacagtctt gcctgatgcg acgctggcgc gtcttatcag gcctgggcag
    31981 tgttagaacc gtaggtcgga taaggcgttt acgccgcatc cgacacgcat tgcacgatgc
    32041 cgcaaaggca taaaaagtcg atggcgttga atattttttc agcgccattt ttattgatgc
    32101 gtgggaagga aatccctacg caaacgtttt ctttttctgt tagaatgcgc cccgaacagg
    32161 atgacagggc gtaaaatcgt gggacacata tggtctggat tgattacgcc ataatcgcgg
    32221 tgattgcttt ttcctctctg gttagcctga tccgcggctt tgttcgtgaa gcgttatcgc
    32281 tggtgacatg gggttgtgct ttctttgttg ccagtcatta ctacacttac ctgtcagtct
    32341 ggtttacggg ctttgaagac gaactggttc gaaatggaat tgccatcgcg gtactgttta
    32401 tcgctaccct gattgttggt gctatcgtga acttcgtgat aggccagttg gtggagaaaa
    32461 cggggttgtc aggcaccgat cgggtgctgg gcgtctgttt cggtgcgttg cgcggtgtgt
    32521 tgattgttgc cgccattctc ttctttctcg actcctttac cggggtgtcg aaaagcgaag
    32581 actggagcaa atcacagctg atcccgcagt tcagttttat catcagatgg ttttttgatt
    32641 atctgcaaag ctcgtcaagt ttcttgccca gagcgtaagt gctctgagat gtggcttaac
    32701 gaggaaaaag acgtatgtgc ggtattgtcg gtatcgccgg tgttatgccg gttaaccagt
    32761 cgatttatga tgccttaacg gtgcttcagc atcgcggtca ggatgccgcc ggcatcatca
    32821 ccatagatgc caataactgc ttccgtttgc gtaaagcgaa cgggctggtg agcgatgtat
    32881 ttgaagctcg ccatatgcag cgtttgcagg gcaatatggg cattggtcat gtgcgttacc
    32941 ctacggctgg cagctccagc gcctctgaag cgcagccgtt ttacgttaac tccccgtatg
    33001 gcattacgct tgcccacaac ggcaatctga ccaacgctca cgagttgcgt aaaaaactgt
    33061 ttgaagaaaa acgccgccac atcaacacca cttccgactc ggaaattctg cttaatatct
    33121 tcgctagcga gctggacaac ttccgccact acccgctgga agccgacaat attttcgctg
    33181 ccattgctgc cacaaaccgc ttaatccgcg gcgcatatgc ctgtgtggcg atgattatcg
    33241 gccacggtat ggttgctttc cgcgatccaa acgggattcg tccgctggta ctgggaaaac
    33301 gtgatattga cgagaaccgt acagaatata tggtcgcttc cgaaagcgta gcgctcgata
    33361 cgctgggctt tgatttcctg cgtgacgtcg cgccgggcga agcgatttac atcactgaag
    33421 aagggcagtt gtttacccgt caatgtgctg acaatccggt cagcaatccg tgcctgtttg
    33481 agtatgtata ctttgctcgc ccggactcgt tcatcgacaa aatttccgtt tacagcgcgc
    33541 gtgtgaatat gggcacgaaa ctgggcgaga aaattgcccg cgaatgggaa gatctggata
    33601 tcgacgtggt gatcccgatc ccagaaacct cgtgtgatat cgcgctggaa attgctcgta
    33661 ttctgggcaa accgtaccgc cagggcttcg ttaaaaaccg ctatgttggc cgcaccttta
    33721 tcatgccggg ccagcagctg cgtcgtaagt ccgtgcgccg taaactgaat gccaaccgcg
    33781 ccgagttccg cgataaaaac gtcctgctgg tcgacgactc catcgtccgt ggcaccactt
    33841 ctgagcagat tatcgagatg gcacgcgaag ccggagcgaa gaaagtgtac ctcgcttctg
    33901 cggcaccgga aattcgcttc ccgaacgttt atggtattga tatgccgagc gccacggaac
    33961 tgatcgctca cggtcgcgaa gttgatgaaa ttcgccagat catcggtgct gacgggttga
    34021 ttttccagga tctgaacgat ctgatcgacg ccgttcgcgc tgaaaatccg gatatccagc
    34081 agtttgaatg ctcggtgttc aacggcgtct acgtcaccaa agatgttgat cagggctacc
    34141 tcgatttcct cgatacgtta cgtaatgatg acgccaaagc agtgcaacgt cagaacgaag
    34201 tggaaaatct cgaaatgcat aacgaaggat gatgccttcg ctgagggtgc cggtctggca
    34261 ccctgacttg caactcccgc cgaaatactg caaagtctgc ctgcaagtct gacagggcaa
    34321 ctatttatga aacgactcat tgtaggcatc agcggtgcca gcggcgcgat ttatggcgtg
    34381 cgcttattac aggttctgcg cgatgtcaca gatatcgaaa cgcatctggt gatgagccag
    34441 gcggcgcgcc agaccttatc cctcgaaacg gatttttccc tgcgcgaagt gcaggcatta
    34501 gctgatgtca cgcacgatgc gcgcgatatt tccgccagca tctcttccgg ttctttccag
    34561 acgctgggga tggtgatttt accctgttca atcaaaaccc tttccggcat tgtccatagc
    34621 tatactgatg gcttactgac ccgtgcggca gatgtggtgc tgaaagagcg tcgcccgttg
    34681 gtgctctgcg tgcgtgaaac accattgcac ttaggccatc tgcgtttaat gactcaggcg
    34741 gcagaaatcg gtgcggtgat tatgcctccc gttccggcgt tttatcatcg cccgcaatcc
    34801 cttgatgatg tgataaatca gacggttaat cgtgttcttg accagtttgc aataaccctt
    34861 cctgaagatc tctttgcccg ctggcagggc gcataactcc gctgttgccc tgtttcaggg
    34921 caattttgca accgcgatca aatcctcgac attttgtttc tgccattcaa tcgaaacgct
    34981 gcgattcaac cgctatacct gctatcttca acttcaggac aataatgcaa cgtcttatta
    35041 acatatttaa cgttgaatgt tactgttgtc gtcaagatgg cataagacct gcatgaaaga
    35101 gcctgcaaac acacaacaca atacacaaca taaaaaagcc attttcactt gagggttatg
    35161 tatgaagaag tcgattctcg ctctgtcttt gttagtcggt ctctccgcag cggcttccag
    35221 ctatgcggcg ctaccggaga cggtacgtat cggaaccgat accacctatg cgccgttctc
    35281 gtcgaaagat gctaaaggtg attttgttgg ctttgatatc gatctcggta acgagatgtg
    35341 caaacggatg caggtgaaat gtacctgggt tgccagtgac tttgacgcgc tgatcccctc
    35401 actgaaagcg aaaaaaatcg acgccattat ttcgtcgctt tccattaccg ataaacgtca
    35461 gcaggagatt gccttctccg acaagctgta cgccgcagat tctcgtttga ttgctgccaa
    35521 aggttcaccg attcagccaa cgctggattc actgaaaggt aaacatgttg gtgtgctgca
    35581 gggatcaacc caggaagctt acgctaacga gacctggcgt agcaaaggcg tggatgtgg
//
LOCUS       DAJVQG010000026        35137 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJVQG010000026 DAJVQG010000000
VERSION     DAJVQG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35137
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.026"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..971)
                     /locus_tag="OQM83_001159"
     CDS             complement(<1..971)
                     /locus_tag="OQM83_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1519468.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSSPDYH
                     GPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEE"
     gene            1063..1149
                     /locus_tag="OQM83_001160"
                     /pseudo
     CDS             1063..1149
                     /locus_tag="OQM83_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1553..2029)
                     /locus_tag="OQM83_001161"
     CDS             complement(1553..2029)
                     /locus_tag="OQM83_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCS1519469.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(2088..3377)
                     /gene="bioA"
                     /locus_tag="OQM83_001162"
     CDS             complement(2088..3377)
                     /gene="bioA"
                     /locus_tag="OQM83_001162"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCS1519470.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3464..4504
                     /gene="bioB"
                     /locus_tag="OQM83_001163"
     CDS             3464..4504
                     /gene="bioB"
                     /locus_tag="OQM83_001163"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCS1519471.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4501..5655
                     /gene="bioF"
                     /locus_tag="OQM83_001164"
     CDS             4501..5655
                     /gene="bioF"
                     /locus_tag="OQM83_001164"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCS1519472.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5642..6397
                     /gene="bioC"
                     /locus_tag="OQM83_001165"
     CDS             5642..6397
                     /gene="bioC"
                     /locus_tag="OQM83_001165"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCS1519473.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6390..7067
                     /gene="bioD"
                     /locus_tag="OQM83_001166"
     CDS             6390..7067
                     /gene="bioD"
                     /locus_tag="OQM83_001166"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCS1519474.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7646..9667
                     /gene="uvrB"
                     /locus_tag="OQM83_001167"
     CDS             7646..9667
                     /gene="uvrB"
                     /locus_tag="OQM83_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCS1519475.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9859..10767)
                     /gene="yvcK"
                     /locus_tag="OQM83_001168"
     CDS             complement(9859..10767)
                     /gene="yvcK"
                     /locus_tag="OQM83_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCS1519476.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      11032..11176
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11164..12153
                     /gene="moaA"
                     /locus_tag="OQM83_001169"
     CDS             11164..12153
                     /gene="moaA"
                     /locus_tag="OQM83_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCS1519477.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12175..12687
                     /gene="moaB"
                     /locus_tag="OQM83_001170"
     CDS             12175..12687
                     /gene="moaB"
                     /locus_tag="OQM83_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCS1519478.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12690..13175
                     /gene="moaC"
                     /locus_tag="OQM83_001171"
     CDS             12690..13175
                     /gene="moaC"
                     /locus_tag="OQM83_001171"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCS1519479.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13168..13413
                     /gene="moaD"
                     /locus_tag="OQM83_001172"
     CDS             13168..13413
                     /gene="moaD"
                     /locus_tag="OQM83_001172"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCS1519480.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13415..13867
                     /gene="moaE"
                     /locus_tag="OQM83_001173"
     CDS             13415..13867
                     /gene="moaE"
                     /locus_tag="OQM83_001173"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCS1519481.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            14004..14708
                     /locus_tag="OQM83_001174"
     CDS             14004..14708
                     /locus_tag="OQM83_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCS1519482.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14913..15626
                     /locus_tag="OQM83_001175"
                     /pseudo
     CDS             14913..15626
                     /locus_tag="OQM83_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
     gene            complement(15691..16402)
                     /locus_tag="OQM83_001176"
                     /pseudo
     CDS             complement(15691..16402)
                     /locus_tag="OQM83_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
     gene            complement(16402..17643)
                     /gene="clsB"
                     /locus_tag="OQM83_001177"
     CDS             complement(16402..17643)
                     /gene="clsB"
                     /locus_tag="OQM83_001177"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCS1519483.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17640..18401)
                     /locus_tag="OQM83_001178"
     CDS             complement(17640..18401)
                     /locus_tag="OQM83_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCS1519484.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18534..18944
                     /locus_tag="OQM83_001179"
     CDS             18534..18944
                     /locus_tag="OQM83_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCS1519485.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18906..20012)
                     /locus_tag="OQM83_001180"
     CDS             complement(18906..20012)
                     /locus_tag="OQM83_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1519486.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20023..21156)
                     /locus_tag="OQM83_001181"
     CDS             complement(20023..21156)
                     /locus_tag="OQM83_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1519487.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21149..22885)
                     /locus_tag="OQM83_001182"
     CDS             complement(21149..22885)
                     /locus_tag="OQM83_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1519488.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKHGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22878..23873)
                     /gene="hlyD"
                     /locus_tag="OQM83_001183"
     CDS             complement(22878..23873)
                     /gene="hlyD"
                     /locus_tag="OQM83_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCS1519489.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23876..24547)
                     /gene="cecR"
                     /locus_tag="OQM83_001184"
     CDS             complement(23876..24547)
                     /gene="cecR"
                     /locus_tag="OQM83_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCS1519490.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24776..26140
                     /gene="rhlE"
                     /locus_tag="OQM83_001185"
     CDS             24776..26140
                     /gene="rhlE"
                     /locus_tag="OQM83_001185"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCS1519491.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(26372..26854)
                     /gene="ybiA"
                     /locus_tag="OQM83_001186"
     CDS             complement(26372..26854)
                     /gene="ybiA"
                     /locus_tag="OQM83_001186"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCS1519492.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26974..29124
                     /gene="dinG"
                     /locus_tag="OQM83_001187"
     CDS             26974..29124
                     /gene="dinG"
                     /locus_tag="OQM83_001187"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCS1519493.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIVIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29152..30114
                     /gene="ybiB"
                     /locus_tag="OQM83_001188"
     CDS             29152..30114
                     /gene="ybiB"
                     /locus_tag="OQM83_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCS1519494.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVATGEAATISDGLARVNQAF"
     gene            30255..31340
                     /gene="hcxB"
                     /locus_tag="OQM83_001189"
     CDS             30255..31340
                     /gene="hcxB"
                     /locus_tag="OQM83_001189"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCS1519495.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(31569..31829)
                     /locus_tag="OQM83_001190"
     CDS             complement(31569..31829)
                     /locus_tag="OQM83_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1519496.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(32094..32360)
                     /gene="ybiI"
                     /locus_tag="OQM83_001191"
     CDS             complement(32094..32360)
                     /gene="ybiI"
                     /locus_tag="OQM83_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCS1519497.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32434..33111)
                     /gene="ybiX"
                     /locus_tag="OQM83_001192"
     CDS             complement(32434..33111)
                     /gene="ybiX"
                     /locus_tag="OQM83_001192"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCS1519498.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33153..35137)
                     /locus_tag="OQM83_001193"
                     /pseudo
     CDS             complement(33153..35137)
                     /locus_tag="OQM83_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8305 a         9011 c         9532 g         8289 t
ORIGIN      
        1 tgctcttcat gttcaaaagc atgccatatc tgtgatacat cagattgttt gttttccggg
       61 aaccatgctg tcacggcatc agccaggggg cgatggagtg tattctgttg tccgtcactc
      121 atggagaagt aaatccgtgg gccgtggtag tccggcgaag aggttaatct ttgcagggct
      181 tgcagagtgc gagtcgacaa tggattgtta taaatattaa catctgcata aatgtgcaga
      241 ttcatgatac tgtctggcaa tgtgttcagc tgattgttag ggatatatag tgattgcaga
      301 cgttgtggaa gttcgggcag tctgtgtagt tggttttccc caacgtttaa aagcctcaga
      361 ttttgaggaa ggggcggtag ctcttgtaat ctgttatcaa aagcacttag ttcctgtagt
      421 tcacatggta attctggtaa ggcagtaagc tggttgttgc caacagaaag tttttgtaga
      481 gccgggggaa gatcaggtag cgtctccagg ctattgagaa gggctgagag tgactgcaag
      541 gaagatggaa gagaaggcaa acatgttaaa actctgttaa gtgaaacatc aagagcgacc
      601 aggtgaggcg gaagagccgg gagcctgctc agtctgttat cgctggcttt aagtacagtt
      661 aaggacggag ggagttctgg cagagagcgt agctcattgc tagagatgtt aagttcttgt
      721 atgtgcgggg gcaggtatgg gagagagcgt aatctgagta aacttaaatt gagggctggc
      781 tcttgaaaag ccagacatat tttcagtaat cgaactgcct gtgttcggtc ttctgttgca
      841 gcaccttcct tggcccagtt atcccagatg cggtgataat ccgtgatatt ttgctcttct
      901 acggataaac gggatataca tcctaaacca caaaaggagc ggaaaaaaga aggattgtgg
      961 gcaggtttca tttattattg atcctcattg tttatatatt aatatgttcg tgcaaatgga
     1021 gtgaggattc tttaaaaaaa cgcgcctgta aatagttaag aacaggaaga actgttgggg
     1081 atgtggctgg ccgaaaatga aggggtgaag ttctggctca atgtgctgac tgaactgaaa
     1141 aaacgcggtt ggtcgcccta ccttttcatc aagccagtag tctgcccacc actgcatcat
     1201 ttctctgcgc ttatcgagat actgagcatg gttgtaaatt cagctaaccc agtgcgttga
     1261 caaaaaatta gcgcaagagg acaaaaaatc accttgcgct aatgctctgt atcaggtcac
     1321 taataccatc taagtggttg attcatagtg actggatatg ttgtgttttg cagtattatg
     1381 cagtctattt tttaagctaa atgtattata atgcattgat attaatgatt tttactgttt
     1441 ctcgttcagc tttttatact aacttgagcg aaacgggaag gtaaaaagac aaaaagttgt
     1501 ttttaatacc tttaagtgat accagatggc attgcgccat ctggcagagt gattaactaa
     1561 acatcgcagt aatcgaggcg cttgccagag agtggaaatg aacgttaaac ccgaccatcg
     1621 cgccgctggc accttcatcg acatcaatac gttctatatc cagcgcgtga acggtaaaaa
     1681 tgtagcgatg agtttcgcct ttcggcggtg ctgcgccatc gtacccggtt ttaccaaagt
     1741 cggtacgcgt ctgcaaaacg ccgtctggca ttgctaccag accagagcca aacccttgcg
     1801 gtaatacgcg ggtatcagcg ggtaagttaa caactaccca gtgccaccag ccggagccgg
     1861 ttggcgcatc cgggtcgtag caggtgacaa caaaactttt cgttcccgca ggaacatcat
     1921 cccacgccag atgcggtgaa atattatcgc catcgtaacc catgccgtta aagacatgac
     1981 gatgcggcaa tttatcgcca tcgcgcagat cgttactgat gagtttcatg aaccctcctt
     2041 tcttgtttgc agaaagtgta gccagaaacc ctcacgcgga cttctcgtta ttggcaaaaa
     2101 aatgtttcat cctgtaccgc gcggttaacc gctgcggtca gacgctgcaa ctgttgcggg
     2161 agaataatat agggcggcat caggtaaatc agtttgccaa aaggccggat ccagacaccc
     2221 tgttcgacaa agaatttttg cagcgccgcc atattcaccg gatgagtggt ttcgaccacg
     2281 ccaatggccc ccagtacgcg cacatcggca accatttcgg catcacgggc gggggcaagt
     2341 tgctcgcgca gctgtacttc aatatccgcc acctgttgct gccagtcgcc agattcgaga
     2401 atcgccaggc tggcgtttgc tgccgcgcag gccagcggat tgcccataaa agttggccca
     2461 tgcataaagc aaccggcttc accgttactg atggtttctg caacctcgcg cgtggtgagt
     2521 gtggcggaaa gggtcattgt gccgccggtt aaggctttac cgaggcacaa aatgtccggc
     2581 gcgatttctg catgttcaca ggcaaacagt ttcccggtac gaccaaatcc agtggcgatc
     2641 tcgtcggcaa tcagcaagat accttcgcga tcgcatattt tgcggattcg ttttaaccat
     2701 tccggatggt acatgcgcat cccgcctgcg ccctggacaa tcggctcaat gatcaccgcc
     2761 gcgatttcat gacgatgcgc cgccatcagg cgggcaaagc ccaccatatc gcgctcatcc
     2821 cattcgccat ccatacggct ttgtggggcg ggagcaaaca agttttctgg cagatagcct
     2881 ttccacaggc tgtgcattga gttgtccgga tcgcacaccg acatcgcgcc aaaggtatcg
     2941 ccatgataac cattgcggaa ggtcagaaaa cgctggcgcg cttcgccttt ggcttgccag
     3001 tactgcaacg ccattttcat cgccacttcc accgctacgg aaccggagtc cgcgagaaaa
     3061 acgcactcca gcggttgcgg cgtcatcgcc accagtttgc ggcacagctc aatggctggc
     3121 gcatgggtga taccgccaaa catcacatgc gacatggcat caatttgcga cttcatcgcc
     3181 gcattaagct gcgggtgatt gtagccgtgg atcgccgccc accaggacga cataccgtca
     3241 accaggcgtc tgccgtcaga caaaatcagc tcgcaacctt cggcgctcac caccggataa
     3301 accggcagcg gggaggtcat ggatgtgtat gggtgccaga tatggcgttg gtcaaaggca
     3361 agatcgtccg ttgtcataat cgacttgtaa accaaattga aaagatttag gtttacaagt
     3421 ctacaccgaa ttaacaacaa aaaacacgtt ttggagaagc cccatggctc accgcccacg
     3481 ctggaccttg tcgcaagtca cagaattatt tgaaaaaccg ttgctggatc tgctgtttga
     3541 agcgcagcag gtccatcgcc agcatttcga tcctcgtcag gtccaggtca gcacgttgct
     3601 gtcgattaag accggagctt gtccggaaga ttgcaaatac tgcccgcaaa gctcacgcta
     3661 caaaaccggg ctggaagccg agcggttgat ggaagttgaa caggtgctgg agtcggcgcg
     3721 caaagcgaaa gcggcaggat cgacgcgctt ctgtatgggc gcggcgtgga agaatcccca
     3781 cgaacgcgat atgccgtacc tggaacaaat ggtgcagggg gtaaaagcga tggggctgga
     3841 ggcgtgtatg acgctgggca cgttgagtga atctcaggcg cagcgcctcg cgaacgccgg
     3901 gctggattat tacaaccaca acctggacac ctcgccggag ttttacggca atatcatcac
     3961 cacccgcact tatcaggaac gcctcgatac gctggaaaaa gtgcgcgatg ccgggatcaa
     4021 agtctgttct ggcggcattg ttggcttagg cgaaacggta aaagatcgcg ccggattatt
     4081 gctgcaactg gcaaacctgc cgacgccgcc ggaaagcgta ccaatcaaca tgctggtgaa
     4141 ggtgaaaggc acgccactgg cggataacga tgatgtcgat gcctttgatt ttattcgcac
     4201 tattgcggta gcgcggatca tgatgccgac ctcttacgtg cgcctttctg ccggacgcga
     4261 gcagatgaat gaacagactc aggcgatgtg ctttatggct ggcgcaaact cgattttcta
     4321 cggttgcaaa ctgctgacca cgccgaatcc ggaagaagat aaagacctgc aactgttccg
     4381 caaactgggg ctaaatccgc agcaaactgc cgtgctggca ggggataacg aacaacagca
     4441 acgtcttgaa caggcgctga tgaccccgga caccgacgaa tattacaacg cggcagcatt
     4501 atgagctggc aggagaaaat caacgcggcg ctcgatgcac ggcgtactgc cgatgccctg
     4561 cgtcgccgtt atccggtggc gcaaggagcc ggacgctggc tggtggcgga tgatcgccag
     4621 tatctgaact tttccagtaa cgattattta ggtttaagcc atcatccgca aattatccgc
     4681 gcctggcagc agggcgcaga aaagtttggt gtaggtagcg gcggttccgg gcatgtcagc
     4741 ggttatagcg tggtgcatca ggcgctggaa gaagaactgg ccgagtggct tggctattcg
     4801 cgggcgctgc tgtttatctc tggttttgcc gcgaatcagg cagtcattgc cgcgatgatg
     4861 gcgaaagagg accgtattgt tgccgaccgg cttagccatg cctccttgct ggaggctgcc
     4921 agtttaagcc cgtcgcagct tcgccgtttt actcataacg atgtcgctca tctggcgcga
     4981 ctgcttgctt ccccctgtcc ggggcagcaa ctggtggtga cagaaggcgt gttcagcatg
     5041 gacggcgata gcgcgccact ggcggaaatc cagcaggtaa cgcaacagca caatggctgg
     5101 ttgatggtcg atgatgccca cggcacgggc gttatcgggg agcaggggcg cggcagctgc
     5161 tggctgcaaa aggtaaaacc agaattgctg gtggtgactt ttggcaaagg atttggcgtc
     5221 agtggggctg cggtgctttg ctccagtacg gtggcggatt atctgctgca attcgcccgc
     5281 caccttatct acagcaccag tatgccgccc gctcaggcgc aggcattacg tgcgtcgctg
     5341 gcggtcattc gccgtgatga gggggatgca cggcgcgaaa aactggtgtc actcattgcg
     5401 cgttttcgtg ccggagtaca ggatctgccg tttacgcttg ctgattcatg cagcgccatc
     5461 cagccattga tcgtcggtga taacagccgt gcgttacaac tggcagaaaa actgcgccag
     5521 caaggctgct gggtcacggc gattcgcccg ccaaccgtac ccgctggtac tgcgcgactg
     5581 cgcttaacgc taaccgcagc gcatgaaatg caggatatcg accgtctgct ggaggtgctg
     5641 catggcaacg gttaataaac aagccattgc agcggcattt ggtcgggcag ccgcgcacta
     5701 tgagcaacac gcagagttac agcgccggag tgctgacgtc ttactggcaa tgctcccaca
     5761 gcgtaaatac acccacgtac tggacgcggg gtgtgggcca ggctggatga gccgccactg
     5821 gcgagagcgt cacgcacagg tgacggcgtt agatctctca ccgccaatgc ttgtgcaagc
     5881 ccgccagaaa gatgctgcag accattatct ggcgggagat atcgaatccc tgccgttagc
     5941 gactgcgacg ttcgatcttg catggagcaa tctcgcggtg cagtggtgcg gtaatttatc
     6001 cacggcactc cgcgagctgt atcgggtggt gcgccccgga ggcgtggtgg cgtttaccac
     6061 gctggtgcag ggatcgttac ccgaactgca tcaggcgtgg caggcggtgg acgagcgtcc
     6121 gcatgctaat cgctttttac cgccagatga aatcgaacag tcgctgaacg gcgtgcatta
     6181 tcaacatcat attcagccca tcacactatg gtttgatgat gcgctcagtg ccatgcgttc
     6241 gctgaaaggc atcggtgcta cgcatcttca tgaagggcgc gacccgcgaa tattaacgcg
     6301 ttcgcaattg cagcgattgc aactggcctg gccgcaacaa caggggcgat atcctctgac
     6361 gtatcatctt tttttgggag tgattgctcg tgagtaaacg ttattttgtc accggaacgg
     6421 ataccgaagt ggggaaaacc gtcgccagtt gtgcactttt acaagccgcg aaggcagtag
     6481 gttatcggac ggcaggttat aaaccggtcg cctctggcag cgaaaagacc ccggaaggct
     6541 tacgtaatag tgacgcgctg gcgttacagc acaacagcag cctgcagctg gattacgcaa
     6601 cagtaaatcc ttacaccttc gcagaaccca cttcgccgca catcatcagc gcgcaagagg
     6661 gcaggccgat agaatcattg gtaatgagcg ccggattacg cgcgcttgaa caacaggctg
     6721 actgggtgtt agtggaaggt gctggcggct ggtttacgcc gctttctgac actttcactt
     6781 ttgcagattg ggtaacacag gaacaactgc cggtgatact ggtagttggt gtgaaactcg
     6841 gctgtattaa tcacgcgatg ttgactgcgc aggcaataca acacgccgga ctgactctgg
     6901 cgggttgggt ggcgaacgat gttacgcctc cgggaaaacg tcacgctgaa tatatgacca
     6961 cgctcacccg catgattccc gcgccgctgc tgggagagat cccctggctt gcagaaaatc
     7021 cagaaaatgc ggcaaccgga aagtacataa accttgcctt gttgtagcca ttctgtattt
     7081 ggttaaattg cgagcgagat cgcgtcttcg attgactgca atttaaccaa ttaaattcta
     7141 aaataatcac gaaaaaaatt ttacttctgc ctcatgcggc gaatgtggga attgcccagg
     7201 cggcggggga taggggctgg agacagttat ccactattcc tgtggataac catgtgtatt
     7261 agagttagaa aacacgaggc aagcgagaga atacgcggct tgcacgcgaa ttggcgttaa
     7321 agacggctca aagaaatatc ttttattttt taactggtta gataaatgca atggcagtca
     7381 ctgaacaggc atctcttgcc ataaaactgt catcactcat cttgacaaat gttaaaaaag
     7441 ccgttgcttt ggggataacc cggtaaggcc ggagttttat ctcgccacag agtaaatttt
     7501 gctcatgatt gacagcggag tttacgctgt atcagaaata ttatggtgat gaactgtttt
     7561 tttatccagt ataatttgtt ggcataatta agtacgacga gtaaaattac atacctgccc
     7621 gcccaactcc ttcaggtagc gactcatgag taaaccgttc aaactgaatt ccgcttttaa
     7681 accttctggc gatcagccag aggcgattcg acgtctcgaa gaggggctgg aagatggcct
     7741 ggcgcaccag acgttacttg gcgtgactgg ctcagggaaa accttcacca ttgccaatgt
     7801 cattgctgac cttcagcgcc caaccatggt acttgcgccc aacaaaacgc tggcggccca
     7861 gctgtatggc gaaatgaaag agttcttccc ggaaaacgcg gtggagtatt tcgtctccta
     7921 ctacgactac tatcagccgg aagcctatgt accgagttcc gacactttca ttgagaaaga
     7981 tgcctcggtt aacgaacata ttgagcagat gcgtttatcc gccaccaaag cgatgctgga
     8041 gcggcgtgat gtggttgtag tggcctctgt ttccgcgatt tatggtctgg gcgatcctga
     8101 tttatatctc aagatgatgc tccatctcac ggtcggtatg attatcgatc agcgcgcgat
     8161 tctgcgccga ctggcggagc tgcaatacgc tcgtaatgat caagcattcc agcgtggtac
     8221 tttccgcgtt cgtggcgagg tgatagatat cttcccggca gaatcggatg acattgcact
     8281 tcgcgtggaa ctgtttgacg aggaagtgga acgattgtcg ttatttgacc cgctgaccgg
     8341 gcagattgtt tccactattc cacgttttac catctacccg aaaacgcact acgtcacacc
     8401 gcgcgagcgc atcgtacagg cgatggagga gatcaaagaa gaactggccg ccagacgcaa
     8461 agtgctgttg gaaaacaaca aactgctgga agagcagcgg ctgacccagc gtacccagtt
     8521 tgatctggag atgatgaacg agctgggcta ctgttcgggg attgaaaact actcgcgctt
     8581 cctctccggt cgtggaccgg gtgagccacc gccgacgctg tttgattacc tgcctgccga
     8641 tgggctgctg gtggtcgatg aatctcacgt caccattcca caaattggcg gcatgtatcg
     8701 cggtgaccgg gcgcgtaaag agacactggt ggagtacggc ttccgcctgc catcagcgct
     8761 ggataaccgt ccgcttaagt ttgaagagtt cgaagcatta gcgccgcaaa ccatctatgt
     8821 ttcggcgacg ccgggtaatt acgagctgga aaaatccggc ggcgatgtgg tggatcaggt
     8881 ggtgcgtcca accggattgc ttgacccgat tatcgaagtg cggccggtgg cgacacaggt
     8941 tgatgatctt ctttcggaga ttcgtcagcg agcggcaatt aacgaacgcg tactggtcac
     9001 cacactgacc aagcggatgg cggaagatct taccgaatat ctcgaagaac atggcgagcg
     9061 cgtgcgttat cttcattcag atatcgacac cgtcgaacgt atggagatta tccgcgactt
     9121 gcgtctgggt gagttcgacg tgctggtagg gatcaactta ctgcgcgaag gtctggatat
     9181 gccggaagtg tcgctggtgg cgatcctcga cgctgacaaa gaaggcttcc tgcgttccga
     9241 acgttcgttg atccagacta ttggtcgtgc ggcacgtaac gttaacggta aagcgattct
     9301 ctacggcgat aagatcaccc catcaatggc gaaagcgatt ggcgaaaccg aacgtcgccg
     9361 tgagaaacag cagaagtaca acgaggaaca cggaattacg ccgcaaggct tgaacaagaa
     9421 agtggtcgat atcctggcgc tggggcagaa cattgccaaa accaaagcga agggcagagg
     9481 aaaatcgcgc ccgattgttg agccggataa tgtgccgatg gatatgtcgc ctaaagcgtt
     9541 gcaacagaaa atccatgagc tggaagggtt aatgatgcaa cacgcgcaga atctggagtt
     9601 cgaagaagcg gcacaaattc gtgaccagtt gcatcagctg cgtgagctgt ttatcgcggc
     9661 ttcgtgacag gataacgaag aagacagatg acaaaagcaa aattgcctga tgtgctacgc
     9721 ttatcaggcc tacatgatct ctgcaataca ttgaattagc gcggtttgta ggccggataa
     9781 ggcgttcacg ccgcatccgg cataaacaac gcgcactttg tcttcaatag gaagccggaa
     9841 tttttcttcg gatttccgtt aacctaaagc ctgtaacgcc ttttccagcg cgttatgtaa
     9901 caactggcgg tcatgacgat acggaatatc gctggcctcc agtacctcct gaatcacaat
     9961 ccgctctttc accgccgaga catccacttt tggcccgacg atgaccgcat caatgacctt
    10021 tttaccaaca tactgctcca taattgccag cttgctttcc agcttcaaat tagccgcagg
    10081 taaactcaac tcacgcccca gattgccgat atagaccatc ggcgctggcg tgcggcgtaa
    10141 tgcctgggcg atttccttca gcagcagaat tggcatcagg ctggtataaa aactgccagg
    10201 cccaataatg atgagatccg cttcattgat agcgtgaacc gcctcacgcg ttgcgggtac
    10261 attcggcgtt aataataact cttgaatcgg cgtagttaac tggtcgatat tgacctcgcc
    10321 gtaaacttca tgcccctgat cgtcaatcgc catcagatcg acgggatgct ctgacattgg
    10381 aatcaaatgc gtatccactt tcagcagatt acgaattaaa ttgatggctt ccagaggccg
    10441 cacgctaagg tgatccagcg cctttaacat caagtttccg agattatgac cggaaagttc
    10501 gccattgccg ccaaaacggt attcaaacat cgcggaggcg acgctcggtt ccgttatcag
    10561 ttggttgagg cagttgcgca tatcgcccca ggcaatgccg ccttctgaac ggcgaatacg
    10621 ccccgtcgag ccaccattat ccgtggtggt gacgataccc gttaaacgag aacccaaaga
    10681 cgaaagtgat gagagaacgc gtcccagtcc atgccctccg ccgagagcaa cgacacgatc
    10741 aagatcagcc agcgtacgat tgcgcatata gtttcctgga gtcagattat ccgcgctaca
    10801 gtagcgcaaa ttgcggggaa acagcaatta acctgccaat attgaggata caaactattt
    10861 tcttctacct ctaaaggacg atgcacgcta tgcctccctg atgatgtata tcaaagtaaa
    10921 actgccattt tcccttattc tgtagcgaaa tagcacgatc atgacgctat atacatgatt
    10981 acatagcgaa agtgtggatg gtaaaaatct catttacacg ctagtatcgg cttaaccact
    11041 aaacactcta gcctctgcac ctgggtcaac tgatacggtg ctttggccgt gacaatgctc
    11101 gtaaagattg ccaccagggc gaaggaagaa atgacttcgc ctcccgtatc tggaaaggtg
    11161 tacatggctt cacaactgac tgatgcattt gcgcgtaagt tttactactt gcgcctgtcg
    11221 attaccgatg tgtgtaactt tcgttgcacc tactgcctgc cggatggcta caaaccgagc
    11281 ggcgtcacca ataaaggctt tcttaccgtc gatgaaattc gccgggttac gcgcgccttc
    11341 gccagtctgg gcaccgaaaa agtgcgcctg acaggaggag agccgtcttt acgccgcgac
    11401 tttaccgata tcatcgccgc tgtgcgggaa aacgacgcta tccgccagat tgcggtcacc
    11461 accaatggtt accgtctgga acgcgatgtg gcgaactggc gcgatgcggg acttactggc
    11521 attaacgtca gtgtcgacag tctggacgcc cgccagtttc acgctattac cgggcaggat
    11581 aaattcaacc aggtcatggc agggatcgat gctgcatttg aggtcggttt tgagaaggtc
    11641 aaagtcaata ccgtgctgat gcgtgatgtt aatcatcacc agctcgacac ctttctgaac
    11701 tggatccagc atcgccctat ccagctgcgt ttcatcgaac tgatggaaac gggcgagggc
    11761 agtgagctct tccgtaagca tcacatctct ggtcaggttc tgcgtgacga gctactgcgt
    11821 cgcggctgga tccaccaatt acgtcaacgc agcgacggtc ctgcgcaagt cttttgccat
    11881 ccagattacg ccggagagat tggccttatc atgccgtatg aaaaagactt ctgcgccact
    11941 tgcaaccgcc tgcgcgtttc ctccattggt aaactccatc tctgcctgtt tggtgaaggc
    12001 ggcgttaacc tgcgcgatct gctggaagac gatacccagc aacaggcgct ggaagcgcgt
    12061 atttcagcgg cgctgcggga gaagaaacag acccatttcc tgcatcaaaa caacaccggt
    12121 attacgcaaa acttatcgta cattggcggc taaaacgtca aaaggagaga tcagatgagt
    12181 caggtaagca ctgaatttat cccgacccgt attgctattc ttacggtttc taatcgtcgc
    12241 ggtgaagaag acgatacctc cggtcactat ttgcgcgatt cggcgcaaga agcgggccat
    12301 cacgttgtcg ataaagccat tgtgaaagaa aaccgctacg ctattcgcgc tcaggtatct
    12361 gcgtggatcg ccagcgacga tgtacaagtg gtgttgatta cgggggggac tggcctgacg
    12421 gaaggtgatc aggctcccga agcattgctg ccgttgttcg accgtgaagt tgaaggtttt
    12481 ggtgaagtgt tccggatgtt gtcgtttgaa gagattggca cttccacgtt gcaatctcgt
    12541 gcggtagcgg gcgtcgccaa caaaacgctg attttcgcca tgccgggttc gaccaaagcg
    12601 tgccgtaccg catgggaaaa tatcatcgcg ccgcagctgg atgcccgtac gcgtccgtgt
    12661 aatttccatc cacatttgaa gaaataagta tgtcgcaact gacccatatc aacgccgctg
    12721 gcgaagcgca catggtggat gtctccgcca aagcggaaac cgtgcgtgaa gcgcgggcgg
    12781 aagcctttgt caccatgcgc agcgagacgc tggcgatgat tattgatggt cgccaccaca
    12841 aaggcgacgt atttgccact gcgcgtattg tcggtattca ggcggcaaaa cgcacctggg
    12901 atctgatccc gctgtgtcac ccgctgatgc tcagcaaagt tgaagtcaat ttacaggccg
    12961 agccggagca caatcgggtg cgtatagaaa ccttatgccg cctgaccggg aaaaccggtg
    13021 tcgaaatgga agcgttaacc gcggcctccg tggcggcgct gaccatttat gacatgtgca
    13081 aagcggtgca aaaagatatg gtgattggtc cggtacgttt gctggcgaag agcggcggca
    13141 agtcgggtga ctttaaggtg gaagcggatg attaaagttc tttttttcgc ccaggtgcgc
    13201 gagttggtgg gaacagatgc aaccgaagtg gctgcggatt acccaaccgt tgaagcgtta
    13261 cgccagcacc tggctgcgca gagcgatcgc tgggcgctgg cgctggaaga tggcaaattg
    13321 ctggctgccg tcaaccagac gctggtgagt tttgaccatc cgctgactga cggcgacgaa
    13381 gtagctttct tcccgccggt aaccggaggt taagatggca gaaaccaaaa ttgttgtcgg
    13441 cccgcagccg ttcagcgtag gagaagagta cccgtggctg gctgagcgtg acgaagacgg
    13501 tgcagtggtc acgtttaccg gtaaggtgcg caaccataac ctgggtgaca gcgtcaaagc
    13561 attaaccctc gaacactatc cggggatgac tgaaaaagca ctggcagaaa tcgttgatga
    13621 agcgcgtaac cgctggccgc tggggcgcgt cacggtgatt caccgcatcg gggaattatg
    13681 gccgggcgat gaaatcgttt ttgtcggtgt caccagtgcg catcgcagca gtgcgtttga
    13741 agccgggcag tttattatgg attatctcaa aacccgcgca ccgttctgga agcgcgaagc
    13801 cacgccggaa ggcgaccgct gggttgaagc ccgggagagc gatcagcagg cggcaaaacg
    13861 ctggtagttt tttgttagcc ggataaggca ccgctgcgtc cggcaatttc accgctcagt
    13921 acatgcgttt acgctgcgtc gatgcacagc ctcatcattt tgcagtatcc ttaagatatt
    13981 ccttatatct tcaggagatc gtcatggaca gattcccacg ttctgattca atcgtacaac
    14041 cccgggctgg cttgcaaact tatatggcgc aagtctatgg ctggatgacc gttggcttgt
    14101 tgctgaccgc atttgttgcc tggtatgcgg ctaattccgc ggccgtgatg gagctgttgt
    14161 tcactaaccg tgtcttttta atcggtctga tcatcgcgca attagcattg gttattgtgt
    14221 tatcagcgat gattcaaaag ctgagcgcag gtgtaacgac gatgctcttt atgctttatt
    14281 cggcgctgac gggtcttacg ctttccagta tattcattgt ctataccgct gcttctatcg
    14341 ccagtacttt cgtcgttact gccgggatgt tcggcgcaat gagcctgtac ggttacacca
    14401 cgaagcgcga tttaagtggc tttggcaata tgctgtttat ggcgttaatc ggcattgtgc
    14461 tggcatcgct ggtcaacttc tggctgaaaa gcgaagcatt gatgtgggca gttacctaca
    14521 tcggcgtgat tgtctttgtc ggattgacgg cgtatgacac tcagaaattg aaaaatatgg
    14581 gtgagcagat tgatacccgc gacacgtcga acctgcgcaa atattccatt cttggcgcgt
    14641 taaccttgta tctggacttc atcaacctgt tcctgatgtt gttacggatc ttcggcaacc
    14701 gccgttaatt ctttttcacc atcactatcc cggccatgtg tcgggatttt tttcttacta
    14761 aaaacgacac ttaacaagta ccaggtataa aatatgcctt atgagcgagc ggaatcgttt
    14821 tccgtatcca taaatacaaa agtattttgt atgcgtggtt ttaaatttat tgatggtgaa
    14881 tattaatatt ttttctaagg acaactaaaa gaatggaatc atactcgcaa aacagcaata
    14941 aattagattt tcagcacgag gccaggatat aaaacggtat atggctcatt accgctttag
    15001 gtttggtggc aaccgcagga ctagcctggg gagctaagta tctcgaaatt acggcaacca
    15061 aatatgattc accaccaatg tatgtcgcta tagggttatt attactttgt atgtatggct
    15121 taagtaagga tatcaacaag ataaatgccg ccatcgcggg cgtaatatat ctgtttttac
    15181 tctctttggt ggcgattgtc gttgcaagtt tagttcctgt atatgccatt atcatcgtgt
    15241 tcagcactgc gggcgcgatg tttttaatca gtatgctggc cggtttatta tttaatgttg
    15301 agcctggttc tcaccgtttt atcattatga tgacgttgac agggttggcc ctggtaatca
    15361 tcgtgaatgc ggcattaatg agtgaacggc ccatttgggt aataagttgc ttaatgattg
    15421 tgttatggcc aggcattatc tcgcatggac gaaataagct ccttgaactg gcaggaaaat
    15481 gccatagtga agagttgtgg agtccggttc gttgcgcttt tacaggtgca ttaacactct
    15541 attactattt tatcggcttc tttgggatac ttgccgcgat agctataacg cttgtctggc
    15601 aaaggcatac gcgttttttt cattagccgt aaatgcacat actaaagaac ttaactaaac
    15661 ttcacatcgc cgcttcattt ttcgcccgta gcccagtaca aagaaatagt tcacgctttg
    15721 accaagtaac agccagataa tcgcccccat taccatccag ttaacgctgg aaatcagcat
    15781 ctgagcgagg gcgaatttcc atgaaggcag caccagcttt tgccctttga tggtcatatg
    15841 gcggtgcttc gcgaaagcgc aaaaccacaa ataaaccgcg ataatcatca gtaagccaat
    15901 gccgagaata cgcagcgtag tttgatcgac ataccagtga tccggcaact ccaccacgcc
    15961 tgcggtaaag ataatccctg ccagtaaaat atagcccagc cagttggtgg taatactgag
    16021 cgagaaaatc cgcgtaatag tgctaccggg caaccccaga cgagagtaca aacgatagcg
    16081 cataccaatg ccgccgaccc aggtactgag cgtcaggttg aaggcgtagc agataaacga
    16141 caccagcatc acctggcgct tcgccagttt gtgaccgcag taaaagcggg cgagcaggtc
    16201 atagcagccg taaatcagat agctgacgac caccagcgac cgcactaagc agcgcaacgc
    16261 gattgtagtc gcggatgacc ttccagacct cttcccagtc cacttttttg gcgtagacca
    16321 ccagcaacac aatcaccgcg ataaaaaaca gccaggtgag gatcttcttt gctaagcgcc
    16381 agcgcgggtg tgatttactc atcagggttt tacccccgtg ttttcagttt ctacccgatc
    16441 ctgcgtttcc attgtcggtt gtgcgggcgg atcaacctgc gccagacgtg gcgtgtgtgc
    16501 cggaagccag ccaaccagcg ccgggaagtg gcgtaaaaag tggaacgcca gcacgctttt
    16561 ggtcaggttc caccaggtgc gtttgggcag catggtttca tccacctgct gacaatctgc
    16621 ggcgataatg ccgttcagat tatcgcgcag cgtctggtta aaattacgat cgtggatgat
    16681 gacatttgct tcgagattca gtgacaaact gagcggatcg agattactgg accctactgt
    16741 cgcccagtga tcgtccatca atgctacttt gccgtggagc gggcggcggc ggtactcaaa
    16801 cacctgaacg ccgcctttaa ccagatagtt atacagcaag cgcgcgccga ctctgacaat
    16861 cggcatatcc ggttcgccct gaatgatcag tttgatccgc accccgcggc gtgccgcttt
    16921 acgcaaggcg tgtaaaaatc gatagccggg gaagaagtag gcgttggcga taatgacttc
    16981 ccgccgcgcc tgagtgagca ttttcagata gtggcgttct atatcatcgc ggtgttcttc
    17041 gttatcgcgc cagaccagca atacctgcgc gtctcccggc tggcggttct cttccgcttt
    17101 gtgatgacgt cgccaccagc gtcgtgccgc gctctgtcca ggcaggtttt ccagctcaaa
    17161 ctggagaata tcttcgacaa tcggcccttc aaggcgtacc gcgtaatcct gtttagcctc
    17221 tggaccgtag ctggacatat gctcggcgga gtaattcagc ccgccaataa aggcgatacg
    17281 cgcgtcgatc accacaattt tgcgatgcat ccggcgaaac acattggtgc gcataccaaa
    17341 aaggcgaggg cggggatcgt agtagcggaa cactacgcca gctgccgtca gttcattgac
    17401 aaactcatcg ctgagatccg gcgaaccgta gccatccagc aagacttccg ctttaacccc
    17461 gcgttgcgct gctgccagta gtgccgcatg cagctgtttg ccgacgttat cctcaaacca
    17521 gataaacgtt tcaagaatga tgcgttcttg tgcctcgcca atcgccttaa acaccgcggg
    17581 ataatattgc tcgccgtttt ccagcaactg gatcttattg ccttcgcgcc agctacattt
    17641 cataaatgaa tctccgcact taaaggggca tgatcagaaa ggtgtcgcca tgtccgcagc
    17701 ggcaacgcgg ttggcgcgct ggcgctggca tttttgacgt agatcctgtc cagtcgtagt
    17761 agaggaaatt gcaccggaaa cgtgcgcgcc gggcgtccgt gggcgcgggt aaaaatctca
    17821 tccagtccgg cctgcacttt taacggatga ttagcttttt gccgccagtc attgaaatca
    17881 cccgccacca ataccggttc gccgtccggt agctcattca cccattcggc gagcatcgca
    17941 agctgcgcct gacggtgcgc ctcacgcagg cccagatgta cgcacatcac atgaatcgct
    18001 tttccggtca tcggcggcac aatgcggcag tagagcacgc cgcgcttttc cgcaccatcg
    18061 accgaaacat cgcgattctc ataatgttca atgggataac gcgacagtac ggcgttgccg
    18121 tgatgccctt ccgggtatac ggcattgcga ccgtaggcaa aatcgctcca catagtgtcg
    18181 gcgagaaact cgtagtgcga ggtatcgggc cagttttcca catgcagcgg atgaacttcg
    18241 tgcgcgccca tcacttcctg caggcaaaca atatcggcgc tgacggtacg cacggcatcg
    18301 cgaagttccg gcaaaatgaa gcgtcggtta aacgcggtaa agcctttgtg aatgttgatg
    18361 gtgagcacct tgaacgaaaa ttgttgtgtt tgatcgggca taattttcct gtctttgcct
    18421 cttatctcat tgaaatagtg tagtcggcgt cacaaaaagg tgcggtctta cggaattttc
    18481 cgtaaagttc ggtactctga gtaagtagag ataaattctt caggagagaa gccatgaagt
    18541 ggcaacaacg tgttcgtgtc gcaacgggtc taagttgctg gcagattatg ttgcatttac
    18601 tggtagtggc gctgctggtg gtgggctgga tgagtaagac tctggttcac gtcggcgtgg
    18661 gattatgcgc actgtattgt gtcacggtag tgatgatgct ggtgtttcag cgccaccccg
    18721 agcaacgctg gcgtgaggtg gcagacgtgc tggaagagct gaccacgacc tggtattttg
    18781 gcgcagcgct gattgtgctg tggctgttgt cccgcgtact ggaaaacaac tttttgctgg
    18841 caattgcagg gctggcgatc cttgctggcc cggcggtagt gtctttgctg gcgaaagata
    18901 agaagttaca tcaccttacg tctaaacatc gcgtacgccg ctgaccctgt cgtggccgtt
    18961 atcaccagta gcggccacaa actattccac acaatatcca gactcgcatc cttcaaataa
    19021 atctgcttgg taatgtccgt aaagtggcga atagggttaa tccacgtcag gttttgcagc
    19081 catactggca tgttttccac cggagaaacg taaccggaaa ggagaatggc gggcatcata
    19141 aaaacaaaca cgccgataaa cgcctgctgt tgtgtcgagc agagtgatga aatcaacaga
    19201 ccgaatccca ccagcgataa accgtaaatc accatcgtaa agtagaacag cgccagcgat
    19261 ccggcgaagg ggatttgata cgcccagata ccaatcgcca gcacaatggt ggcctggaag
    19321 gtggcgacaa ttaacgccgg tacggctttg ccgatgaaga tctgccaggt ggtgagcggc
    19381 gaaaccagta gctgatcgag cgtaccttgt tcacgttcgc gggcgacgga aagtgaagtg
    19441 acgatcatta cgccgatagt ggtgatcatg gcgatcagcg acggcaccac aaaccatttg
    19501 tagtcaagat tcgggttata ccagttgcgt accaccagct cgctgttgtt aggtttcggt
    19561 tttccttcca gcagctcctg ctgataattt ttgacgatct gttgcaggta gttggcggca
    19621 atttgcgcac tgttggagtt acgcccgtcg aggatcaact gcaacggcgc ggtctggaag
    19681 gtatccagtt tgcgcgagaa gtcagccggg aaacgcacca gtagtaacgc cttttgtgtg
    19741 tcgatggttg ggcggatctc ctgtgggctt ttcagcagca gcacatgggt aaaggcgctg
    19801 gcgcgggcaa aacgttgggt cagctccacc gaatgctcgc cgttatcttc atcgtagatg
    19861 gcgatggtgg cgttagtcac ttccagcgtg gcggcgaacg ggaacaggat cacctgaata
    19921 agcacaggta aaatcagaat cgcgcgggtt tgtggttcgc gcagcaacga ctgcaactct
    19981 ttgcggatta acgtccataa gcgatgaaac atgctcttct ccctaatcca gccgacgttt
    20041 ggttttcagc cacgtcaggc cgataaacat caccgccgaa gcgatcaaaa acagcacgtt
    20101 taccaccagc accactggaa tattcccggc gaggaacagg ctttgcaggg tgctgacgaa
    20161 ataacgagcg ggaataatgt acgtcaccgc gcggatcacc gcgggcatac tgtcgatctg
    20221 aaaaataaag ccggaaagca taatcgacgg caggaacgcg gcgttcaggg cgacctgagc
    20281 ggcattgaac tggttgcggg taatcgtgga aatcagcagc cccatcccca gggtactgag
    20341 taaaaacagg ctggagataa aaaacagaat aagcagcgaa ccgcgatacg gcacgccgag
    20401 aataaacact gacaccagca tacacagcag catcgccagc atcccgagaa agtaataagg
    20461 gatcagctta cacagcagca gttccgtgcg ggtaatctcc gtagagagca gagcctccat
    20521 ggtgccgcgt tcccattctc gcgccaccac cagcgaggtg agaatcgcgc cgatgaccgt
    20581 catgatgatg gtcactgcac cggggataat gaagtgctgg ctaatcgccg ccgggttaaa
    20641 ccagtagcgg gtttgtacat caataagcgg ttcaaaagtc tgcccgttgt cctccgctcg
    20701 ctgcatttgc cagatctgcc agatcccttc gacatacccc tgtacaaagt tagcggtatt
    20761 cggctcactg ccgtcggtga tcacctgaat cggtgcggtg gcgttggcgc gctccatctg
    20821 ttccgcaaaa tccaccggaa taaccaccag accgcgaatt ttccccgcct gcattttggc
    20881 gatcagttcc tgacggttat cgctgatggt ggcgtcgatg tagggcgaac cggtcatggt
    20941 gtgggtgaaa tccagcgccg cttcgctacg ctgttccagc aagatcccga cccgcagctt
    21001 gctggagtcg aggttaatgc cataaccaaa aataaacagc agtagcagtg ggatcactac
    21061 cgcaatcagc cagctactcg gatcgcgaac gatctgccgc gtctctttga cgcacagcgc
    21121 ccgcacgcga cgccaggaaa ggatcgggtt actcattgct atgctcctta tcccagtcgt
    21181 ggatcaactg aataaaggct tgctccatgg tgggatcggg ttgctcatcg ttagccgact
    21241 gtgctttcaa atcgtccggc gtaccgctgg cgattaattt cccacggtac accaggccga
    21301 tgcggtcgca atattccgct tcatccataa agtgggtggt gaccatcacc gtgacgcctt
    21361 tctctaccat gctgttgata tgtagccaaa attcacggcg ggtgagggga tcaacgccgg
    21421 acgtcggttc gtcgagaaac agaatgtccg gttcatgcat cagcgaacag gccagcgcca
    21481 gccgctgttt aaaacctaat ggcagttcat cggtggcgtg ggaggcgata cttttcaggc
    21541 cgaacgcctc gctcatgcgg gagatttttt cgttctgcgc ccgaccgcgt aagccataca
    21601 caccagagaa aaagcgtaaa ttctgttcga ccgtcaggtt accgtagagc gaaaactttt
    21661 gcgccatata gccgagatgc tggcgcgctt taccagaact ctctttcaga tccatcccca
    21721 gtaccagcgc ctgaccagag gtcggcacca gcaaaccgca catcatctta aaggtggtcg
    21781 atttgcccgc gccgtttggc cccagcaaac caaaaatctc cccgtgttta acggcaaagt
    21841 tgacgtgatc ggtggcggca aaatcaccaa atttcttggt cagttctttc gcttcgatca
    21901 ccgtctcgcc gggtgtgcct tctaccgtat gtaatattgc gcccagcggc gattccgagg
    21961 ttccggcacc gcccagcaaa tcaataaacg catcttcaaa acgcggcgta gtttcgttga
    22021 tgttgatttc cggcatcccg tcggcatggc gaatatcgtc tggtgtggtc tcttttttga
    22081 ggatcagacg taccgatttc ccctgaatca tgccgtcgct gacctgcggc agtttcaatg
    22141 cccgttgcaa cagtttgcgg ttgccctcgt gtggacttgt catcagaaag ctgcgtccgg
    22201 ccatggtttg tgtcagggct tttggttccc cctgatacag caactcgcct tcattcatca
    22261 gtaacacgtc acggcactgc tcggcttcgt cgagatacga ggtactccag aggattaaca
    22321 tcccttcgcc tgccagctca tgcaccatct gccacagttc gcgccgtgag atagggtcaa
    22381 cgccgacgcc gggttcatcg agcagcaaca ctttcggttc gcccaccagg gtacaggcca
    22441 ggccgagttt ttgtttcatc ccaccggaga gcttgcccgc caggcgtccg gtaaacggcc
    22501 caagagacgt aaactccagc aggcgagcaa aagtttgctt acgtgcctcg ccggtgacgc
    22561 tgcgcaaatc cgcgtacaga ttgaggttct ccatcaccgt gagatcttca tacagaccaa
    22621 atttctgcgg catatagccg agcacggcgt gcagcgcgcc gtcgtttttg attggatcaa
    22681 agccaatcac cgtggcattg ccgctgtcgg gtttcagtaa tcccgccagc atccgcatta
    22741 gcgtggtttt acctgcacca tccggcccca ccagccccgt cacataaccg gcgtgaatgg
    22801 tacaatcgag cggcgcgacg gcgggcttgt ccatgcccgg aaagcgtttt tccaggccgt
    22861 tcagcgtgat aacggcatca ttcatgtcct gcctcgttac cgaattgcac cgtcaccggc
    22921 attccctggc gtaacgcatc atcggcgtcg gtcaccacaa tacgcaggcg atagacgagg
    22981 tcggtacgca gatccggtgt ttcgacggtt ttcggggtaa attcagcagt cggcgaaacg
    23041 aaaccaatct gcccgtgata cggcttgtcc gggcgaccat cggtataaag cagcactttg
    23101 cgccccggct gggcctggtc aagataccgt tcatcaacat aagcgcgcac ccacaccgga
    23161 cgcgttagtg aaacggtaaa caccgtgcca ccttcattga ggaccgtgcc tggctccacc
    23221 gcgcgcgtta acagcgtgcc atcagacggg gctatcaacg ttgagtcctg taaattcaac
    23281 tccgcctgcg ccagttgcgc ctgcgcctgt tcgaggctgg ctttcgcctg ggcgatgtcc
    23341 tgttcacggt taccggaacg gtactgacgc aatttatcct gtgctgattt cagcgttgcc
    23401 tgcgcctggt cgcgcgagga gcgggcattt tccaggtcat ttgccgaaat agtgcggctt
    23461 ttccacagtc cttgctggcg gttatagaag ttctgcgcat agtcataggc ggcttgcgcc
    23521 tgtttcaccg ctgcggcggc ctgagcgatt tcttcatcgc gatacccggc aagcatcaga
    23581 tcatactgcg cctgtgccac cgagacgccc gctttcgcct gcatcagggc aatctcatac
    23641 ggcttgtgat ccagttcgcc cagcacctgg cccgctttga tagcatcacc ttcgtccacc
    23701 gccagcgatt caacgcgccc cccaacacgg aaactaagat tcaccgtacg aatatccacg
    23761 ttgccataca gcgtcaggcc gttatcctgg cggctttgat accaccagta gcctccggca
    23821 accacggcgg caagtaccac taccgccagt tcgatcacga caggtttttt catcactaca
    23881 aactcctttg cgataatcct tgcaaaatca ggtcgatatg acaggtcacc gtctggttga
    23941 tcagttcggt cttttcttca tcgaacgcgg tccagccggt acgtaacaga attgtttctt
    24001 tgccaagacg gaacgccaga atctcaccaa tcaacgcatg ggtatgaagg atcatgcggg
    24061 tgtcattggc gtcgctgccg gtccaggcgg caatcaggcg tgtcaggtgg ctgtgtagcg
    24121 gactaatcac ctgttcgtgc accaggtggt aggctgccgt gggagagagc tgctcacggg
    24181 agataaactt gctgaggttg acggtgtcat cctgggtgag cagcttaatc atgttcctgc
    24241 aggcgcgaag gagcagttca cggatggcag cccgatcagg ctgtggttgg gcgaacaagc
    24301 gttcggcttc ctcggcatgc ggacggaact gctcgccaat aaaatcggca atccactggg
    24361 cgcaggcgag gtacaaatct tctttcgaac cgaagtagta ggtgatggca gcgatattct
    24421 gcccggcttg ggcggctatc tcgcgggtgg tggcgttcat tccatattca ccaaactgcg
    24481 ccagtgcggc agcaatcagc tgttttttcg cctgttcacc cttgattgtc atggcaggat
    24541 tattcatcgc acagtccatt cttaatcaaa tgattgatta agattatgac tccatatggg
    24601 agttgtccag tatggctaag aattttagct acgccagtca cagggataat ttatgcgctg
    24661 cgtcacaaaa actgctacac tccgctcgct catgacattg tggtttttgt cattttcctt
    24721 ttcagtatct ccctgaaaac tacaccggta acggtcgggg cggttcggag tagtcatgtc
    24781 tttcgattat ttgggtttaa gccctgatat cctgcgcgcc gttgccgagc agggttaccg
    24841 tgaacccacc cctattcagc agcaggcgat ccctgcggtg ctggaaggcc gcgacctgat
    24901 ggctagcgcc cagaccggca ccggcaaaac agcgggcttt acgctgccgc tgttgcaaca
    24961 cctgatcact cgccagccgc acgccaaagg gcgtcgtccg gtacgtgcgc tcattcttac
    25021 cccgacccgt gaactggcgg cgcagattgg cgaaaacgtc cgtgattaca gcaaatacct
    25081 gaacattcgt tcgctggtgg tgtttggtgg tgtgagtatt aacccgcaga tgatgaaact
    25141 gcgtggcggc gttgatgtgc tggtggcaac gccgggacgt ttgctggacc tggaacatca
    25201 gaatgcagtg aagctggatc aggttgaaat cctcgtcctc gatgaagctg accgcatgct
    25261 cgacatgggc tttatccacg atatccgtcg cgtgttaaca aaactacctg cgaagcgcca
    25321 gaacctgtta ttctccgcga ccttctctga cgatattaaa gccctggcgg aaaaactgtt
    25381 gcacaacccg ctggaaatcg aagtggcacg ccgcaatacc gcgtctgatc aggtgaccca
    25441 gcacgttcac tttgtcgata agaaacgcaa acgcgaattg ctgtcgcaca tgattgggaa
    25501 agggaactgg cagcaggtgc tggtgtttac ccgtaccaaa cacggcgcta accatctggc
    25561 tgaacagctc aataaagatg gcatccgtag tgcggcgatc cacggcaata aatcgcaagg
    25621 tgcgcgtact cgtgcgctgg ctgattttaa atcgggcgat attcgtgtac tggtggcaac
    25681 tgacatcgct gcgcgcggcc tggatattga agagctgccg cacgtcgtca actatgaact
    25741 gccaaacgta ccggaagatt atgtccatcg tatcgggcgt accggtcgtg cggctgctac
    25801 cggtgaagcg ttgtcgctgg tgtgtgttga tgaacacaaa ctgctgcgtg atatcgaaaa
    25861 actgctgaaa aaagagatcc cgcgcattgc gattccgggc tatgagccgg acccgtcaat
    25921 caaagccgaa ccgatccaga acggtcgcca gcaacgtggc ggcggcggtc gtgggcaagg
    25981 tggtggtcgc ggtcaacagc aaccacgccg tggggaaggt ggcgcaaaat ctgcaagcgc
    26041 gaaacctgca gaaaaaccgt ctcgccgcct cggcgatgcc aaaccggcag gcgaacaaca
    26101 acgtcgccgc cgtccgcgta aacctgccgc tgcgcagtaa tcttttatgc cgggctatgc
    26161 ccggcatcag gctgatgaac aaacgcaaaa ctgcctgatg cgctacgctt atcaggccta
    26221 cgtgaactct gcaatatatt gaatttgcat gcttttgtag gccggataag gcgtttacgc
    26281 cgcatccggc atttcacaac aagcacttgt cagcaatttg agaacacggg aaaaaattta
    26341 tctgttttac cgcccataag ccaccgagac gttacttctc tatagccaat tgttctcgca
    26401 actccattaa aaggtagccc agtcgattct tgcccttacc attaccaccg tctccccagt
    26461 aagcatcgtt ttccgtatgc tcaaccagtt ttgcgggcgc ggttgccagc aagagcacac
    26521 gcagttctgc atgctgttcg aatttagcac gaagtgcttt acgcatcacc tgttctttaa
    26581 ccgactccca gtttttacga agtggcttgg aacgattacg ccccatgcga gcggcgacca
    26641 tgggggaaga aacccgacgg atctcttcgc ggtatttttc atcaaggaat ttttgtgcct
    26701 gaaaatagtg ttcggaggtg ggccaggttt tcccgtcaac cttgatgggc caggcggcaa
    26761 agttagaaaa atcaccgtag tcatcgctgg tgctgtagaa atttatgatg gtgtcttgca
    26821 tgacgtgttg gatcctttgt gctcgaacgg gcattaaacc gcattatgtt ggtggttatt
    26881 gcgagccgct ttccagaaac agaaaaacca ttacccctga aaaccgaaaa atgccacaat
    26941 attggctgtt tatacagtat ttcaggtttt ctcatggcat taaccgccgc gcttaaagcg
    27001 caaattgccg cctggtataa ggcgcttcag gaacagatcc ccgactttat tccccgtgtg
    27061 ccgcagcggc agatgattgc ggacgtcgcc aaaacgctgg ccggagaaga aggccggcat
    27121 ctggcgattg aagcccccac cggcgttggg aaaacgctct cctatttgat ccccggcatc
    27181 gccattgccc gcgaagagca aaaaacgctg gtggtgagta ccgccaacgt ggcgttgcag
    27241 gatcagattt acagcaaaga tttaccgctg ctgaaaaaga tcattcccga tcttaaattc
    27301 actgccgctt tcgggcgtgg gcgctacgtt tgtccgcgta atctgacggc gctcgccagc
    27361 actgaaccca cgcaacagga tctgctggcg tttcttgacg acgaactgac ggcaaataat
    27421 caggaagagc aaaaacgttg tgcgaagctg aagggcgatc tcgacactta tagatgggat
    27481 ggtctgcgcg atcatacgga tatcgttata gatgatgatc tctggcgtcg tttgagtacc
    27541 gacaaagcca gctgcctcaa ccgtaactgt tactactatc gtgaatgccc gttttttgtt
    27601 gctcgtcggg agattcagga agcggaagtg gtggtggcaa accatgcgct ggtgatggcg
    27661 gcgatggaaa gcgaagccgt actgcctgac ccgaaaaatc tgctgctggt gctggacgaa
    27721 ggtcatcacc tgccagatgt ggcccgggat gcgctggaga tgagcgctga aatcaccgcg
    27781 ccctggtatc gactacagct ggacttgttc acgaaactgg tcgctacctg catggagcag
    27841 tttcgcccga agaccatccc accgctggcg atccctgaac gtttgaatgc gcattgtgaa
    27901 gagttgtatg agcttatcgc ctcgttaaac aacattctca atctctacat gcctgccggg
    27961 caggaggcag agcaccgttt tgcgatgggc gaactgcctg atgaagtgct ggagatctgc
    28021 cagcggctgg caaaactcac cgagatgctg cgtggtctgg cggagttatt tcttaacgat
    28081 ttaagtgaga aaaccggcag ccatgacatt gtacgcctgc atcggttgat tttgcagatg
    28141 aaccgcgcgt tggggatgtt cgaggcgcaa agcaaactct ggcggctggc gtctctggcg
    28201 caatcttccg gtgcgccggt gaccaaatgg gcgacgcggg aagagcgcga agggcaactg
    28261 catctctggt ttcactgcgt ggggatacgt gtcagcgatc agctggaaag gctgctgtgg
    28321 cgcagtattc cgcacattat tgtcacctcc gcaaccttgc gttcgctgaa tagtttttcg
    28381 cgtttgcagg agatgagtgg tctgaaagag aaagcgggcg accgttttgt ggcgctggat
    28441 tcccccttta accactgcga acagggcaaa attgttattc cccggatgcg cgttgagcct
    28501 tccatcgaca acgaagagca gcatattgcc gaaatggcgg cctttttccg taagcaggtg
    28561 gagagcaaaa aacatctcgg tatgttggta ctgtttgcca gcggacgggc gatgcagcgc
    28621 tttctcgact atgtgacgga tttacgtctg atgttgctgg ttcagggcga tcagccgcgt
    28681 taccgtttag ttgaactgca ccgcaaacgc gtcgccaacg gtgagcgcag cgtgctggtg
    28741 ggcttacagt catttgccga agggcttgat ttgaaaggtg atctgctcag ccaggtgcat
    28801 atccacaaaa tcgcttttcc gcccatcgac agccccgtgg tgatcaccga aggggaatgg
    28861 ctgaaaagcc tcaaccgcta tccgtttgag gtgcaaagcc tgccgagcgc ctcgtttaac
    28921 ctgattcagc aggttgggcg actgattcga agccacggtt gctggggcga agtggttatc
    28981 tacgacaaac gcttgctgac caaaaattat ggcaagcgac tactggatgc attaccggta
    29041 tttccgatag agcaaccgga agtccctgaa ggtatagtta aaaagaaaga aaaaacgaaa
    29101 tccccacgcc gtcggcggcg ttaatgatgt gagtcaggta aggagtcgta aatggactat
    29161 cgcaaaatca ttaaagagat cgggcgcggg aaaaaccacg cgcgcgattt agaccgggat
    29221 actgcgcgcg gtctgtatgc tcatatgctc aatggtgaag tccctgacct tgagttgggc
    29281 ggcgtattga ttgcgctgcg tatcaaagga gaaggggaag cagagatgct cggcttttac
    29341 gaagccatgc aaaatcacac catcaagctg acgccgccag caggcaagcc gatgccgatt
    29401 gtcatcccca gttacaacgg cgcgcgtaaa caggccaacc tgacgccgtt gttggcgatt
    29461 ctcctgcaca aactcggttt tcctgtggtg gttcacgggg ttagcgaaga tccaacccgc
    29521 gtgctgactg aaaccatttt tgaattgatg ggtattacgc caacgttgca cggcgggcag
    29581 gcgcaggcga agctcgacga acatcaaccg gtgtttatgc cagtcggcgc gttttgcccg
    29641 ccgctggaaa aacaactggc gatgcgctgg cggatgggcg tgcgtaacag tgcacatacc
    29701 ctggcgaaac tggcgacgcc atttgccgaa ggtgcggcgc tgcgtctttc cagcgtttcg
    29761 catccggaat atattggacg cgtcgcgaag ttctttagcg atatcggcgg gcgggcgcta
    29821 ctaatgcatg gtactgaagg tgaagtgtat gctaatccgc agcgctgccc gcagatcaat
    29881 ctcattgacc gtgaagggat gcgggtgctg tatgaaaaac aggacactgc tggtagcgag
    29941 ttactgccac aagcaaaaga tccggaaacc acggcgcagt ggattgagcg ttgccttgct
    30001 ggcagcgaac cgattcccga atcgctgaaa atccagatgg tttgctgcct ggtggctacg
    30061 ggtgaagcgg caactatcag cgacggcctg gcgcgcgtta atcaggcatt ttaattcttt
    30121 tctcagccgg atgagccatg tcatccggct tttaccccac cgcaatatga aattcctgca
    30181 tctttattga ccttcccacg cccggcgtgc agcataaaaa tacaacaaac acataacata
    30241 aacaggagtt aaccatggaa agtggtcatc gctttgatgc tcagacgctg cacagtttta
    30301 ttcaggctgt atttcgtcag atgggtagcg aggaacaaga agcgaaatta gttgccgatc
    30361 atttaatcgc ggcaaacctg gcagggcatg attcacatgg tattggcatg atcccaagct
    30421 atgtacgctc ctggagtcag gggcacctgc aaattaacca tcatgccaaa accgttaaag
    30481 aggcgggggc ggcggtcacg ctcgatggcg atcgcgcatt tggtcaggtc gcggcacatg
    30541 aagcgatggc gctggggatt gagaaagcgc atcagcacgg tattgccgcc gtggcgctac
    30601 ataactcgca tcatatcggc cgtatcggtt actgggcgga gcagtgtgca gcggcggggt
    30661 ttgtctctat ccactttgtt agcgtggtcg gtattccaat ggtcgcgccg ttccacggtc
    30721 gcgacagccg ctttggcacc aatccgttct gtgtggtttt ccctcgtaaa gataatttcc
    30781 cgctgttgct tgattacgcc accagcgcca ttgcatttgg caaaacccgc gtcgcctggc
    30841 ataaaggcgt ccccgtgccg ccaggttgcc tgattgacgt taacggcgtg ccgacgacca
    30901 atccggcggt aatgcaggag tcgccgttgg gttcgctgtt gacctttgcc gaacataaag
    30961 gctacgccct tgcagcgatg tgtgaaattc ttggcggggc gctttccggc ggtaaaacga
    31021 cgcatcagga aacgttacaa accagtcccg atgccattct taactgcatg accactatca
    31081 tcatcaaccc ggaactcttc ggcgcgccgg attgtaacgc gcagaccgaa gcctttgccg
    31141 agtgggtgaa agcctcgcca catgatgatg ataagccgat tttgctaccg ggcgagtggg
    31201 aagtgaacac gcgtcgcgaa cggcagaagc aggggattcc actggatgcg ggaagctggc
    31261 aggccatttg tgatgcagcg cggcagattg gtatgccgga agagacgttg caggctttct
    31321 gtcagcagtt agccagctaa aaaaaagccc gtccagtggc ggacgggcaa acaagggtaa
    31381 cataggatca atgagggtta gagcatatgc gtctgtcggc aaacagacag ggaaatactt
    31441 gtgctggacg tagcgtaaac gcctgatccg tccaacggtt cggattttgt aggcctgata
    31501 agacgcggta gcgtcgcatc aggcatctgt tgccggatgc ggcgtaaacg ccttatccgt
    31561 cctacgaatt acttataaat taccgcagta ccgcttaatt tattgttgtt ggtggcggaa
    31621 gtgatgctgt atccactggc accggctgcc gcggctttct cagccagttt cgcttccagg
    31681 gcatcgaggg tggatgcgcc atcggcagaa accacgccga ttttattcat gttctgtgcc
    31741 tgggatgccg ttatcggttc cgccgcgaac acgccaaatg acagagttga aagagccata
    31801 gcagcaacaa cagtattgat agttttcata attaatctct cgcaggtgat ttttgtataa
    31861 gtgacgttgt ttcgtcgatg tgatgagtat cacgtttttt tacgagagat aaaatcgaag
    31921 agaattgacc gccttgttca aataaattga ttgatatcta acatactgat aaaccattta
    31981 tttgttgtac ttatgaactt gttttatacc gcgtggcagt cacagcagcg ttaaaggcac
    32041 ggaatgccac gcaaaataaa aattatactc gctatgcatc tcgccagttg tagttaacgt
    32101 aactggctgt ctttcgaacc tctgcgatta tatcctgtat aagctggttt ttgtaaatct
    32161 ttctcctgct gacaatgaat acataagcgc acgccaggaa tggcttcccg acgggcctgc
    32221 gggatggggg caccgcactc ttcacattca tccaggcttt cgccgcgcgg aatttcaccc
    32281 cgagcgcggg caatcgcatc ttcaattgta ctgttgatct gttcgttgac ggcgtcatcg
    32341 ttagcccaac cggatgccat atcgacctcc ccatatcaat acttgtacag ttaagtgtag
    32401 ctaatccagg gacgaactcg ggcagttcaa gcatcagatc tccgaccatt cccgcagcag
    32461 attatgataa agattaagca gcgacaggat ctcttcactt tcgccgtagc ggcttttcag
    32521 cgactgaata ttgttgtcca gttcaaacag catggcgcgc tttttatcat cgcggatcat
    32581 cgactggatc cacataaatg atgccactcg tacgccgcgg gttacgggtg gcacgcaatg
    32641 ctggctgctg gagggataca acacgagatc gcctgccggg agttttaccc gatgttgtcc
    32701 gaaggtgtca ttaacgacca gttcgccgcc gtcgtagctt tgtggatcgc ttaaaaacag
    32761 cgtggcagaa aggtcagtac gcatccagcc gttttgtgga tggctgcgta ctgcgccatc
    32821 cacatggaaa ccataggttt cattgttctg atagcgatta aacagcggcg tggaaagggt
    32881 acgcggcaag gccgcggcaa agaacaaagc atgttggtta accgcgttca gcacctcatt
    32941 ttgcagggcg gcgtataacg tgctgcgggt gtcgacctgt tgattgttct taacttgcgc
    33001 accttgtgcg ccggtggtga cgcgtccatc cacccattcg gcttgttcca gttgttcgcg
    33061 aaaccgggcg acgtcctgtg gcgataacac gccgggaatg tggtacatca tacaagttct
    33121 ccaaaaagtg gggcctgcgc cccacatctg aatcagaaat gcatattggc tgtgagcaag
    33181 aaggttcttg gctcgcccgg gtgataacgg tagccgctct tgttgattga ggcgacgtaa
    33241 tcagtatcaa acaggttgta gacgtttagc tggaagtcga gattgcgatt aacccgatac
    33301 cccagtttgg catcggcgac ccagtaacct tcggtaaatg ctggcgttcc caccgcgccg
    33361 tctgaacctt tatgcatact gccgatatag cgtgcgcccg cgccaacaga gatatcgtcg
    33421 gttgcctgat attggctcca taaggtgaag gcgtgctccg gggtatacgg cagcgatgag
    33481 gaaccatcct gggcaacatc tttgccgttt ttgatggttg ctttttgctg ggtatagccg
    33541 ccaatcacct gccacgcggg agtgatattc ccggccacgg atatctcata gccttcgacg
    33601 cgtttcttac cgtattgcga gtaagttccg tcatcatttt gctcaacttc attttcgata
    33661 tcagtacgga acagcgcggc ggtgagcaac agacgtttat ccagaacctg ccatttggtg
    33721 ccaatctcgc tggtgttggc tttttgcggt ttaaaatcgg tgcggttggc actgttaccg
    33781 ctgccagact gcgcaagggc gaagttgttg ccgcccggag gctgctggga aacggcatag
    33841 ttaatataga cattgccgtt ttccgtcagg tgatacagcg ccccggcttt ccagttcatc
    33901 agattgcccg acttggcggt gtcgacggtg gtgaccggag aaccttttgc cacaccagtt
    33961 gggcaggtga tggcaccgcg tccgctgccg ccgcaggcgg tggcactgtc atattcagta
    34021 tgataattat ccagacggat cccgccgttc agctcaaaat cacgggtgat ttgcagcgta
    34081 tcaaaggcgt aaattgcgaa ggtatccgtc tgaccattgg cgtttgcgcc gttgcgcgtc
    34141 aggccgccgg gatgaatgct gctgtcagga tgataaatat ttaccgcggg taacgtcacc
    34201 ggattaacgc cgtagttagt ctgcgtttca cgggtaaatt ccacgccggt actgacatca
    34261 tgaccgatag aaccggtata gaacgtcgag gtcaggttgg tctggttggt gagaatttta
    34321 ttactcacat ctttggtatt cgccgtgcgt gaccaggttc agctattcac atcgctggtg
    34381 ggctgagtaa tattcgacgc cccgcccata atcgccgtca tcaggtaatc ctgctttacg
    34441 cgcgaccaac gggtagtatt gcgaatggtg gtgttatcgt tgatgtcgtg ctcaaaacgc
    34501 atggtggcgg tgtcggtggt cgaatcgtcg taatcggaat ccgtgccgta aaagttatga
    34561 gtatcaactt ttccggaatg attcagggcc gccgttcccg cagatggggc agaatagccc
    34621 ggcaaaccga tggtcggaat gccgccgtct ggcgtgttgt gctgggtgac atgcagataa
    34681 ttaagataca aacgattcgc tgtaccaagg ccaaaagcga cagaaggggc gacgccgtaa
    34741 cgctcatttt tgactttgtc gcgtccggca tcgtgcgttt tttcgcccat tacattcagg
    34801 cgcaccgcgg tggtatcacc aatgacctga ttgacgtcca gcgtgccgcg gcggaaccag
    34861 gcgctgccaa tactggcgga ggcgtcaatg ccggaatcat tgcgcggctg tttgctgatc
    34921 atattgatcg agcccgtcgg tgcgctgcgc ccgtagtcgg tgccggacgg ccctttaatc
    34981 acttcgacct gctcggtatt gaaggtgtcg cgcgagacgc tgccgatatc gcgaatgcca
    35041 tcaatataaa tactgttaga ggtatcggca ccacgcatat aaatggcgtc gccagtggtg
    35101 gagttaccgt tctcacccgc aaaaaacgca cccacgc
//
LOCUS       DAJVQG010000027        33995 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJVQG010000027 DAJVQG010000000
VERSION     DAJVQG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33995
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.027"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(1..2601)
                     /gene="acrF"
                     /locus_tag="OQM83_001194"
                     /pseudo
     CDS             complement(1..2601)
                     /gene="acrF"
                     /locus_tag="OQM83_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(2613..3770)
                     /gene="acrE"
                     /locus_tag="OQM83_001195"
     CDS             complement(2613..3770)
                     /gene="acrE"
                     /locus_tag="OQM83_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HCS1519499.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            4169..4831
                     /gene="acrS"
                     /locus_tag="OQM83_001196"
     CDS             4169..4831
                     /gene="acrS"
                     /locus_tag="OQM83_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HCS1519500.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(4834..5013)
                     /locus_tag="OQM83_001197"
     CDS             complement(4834..5013)
                     /locus_tag="OQM83_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCS1519501.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(5097..5981)
                     /gene="yhdJ"
                     /locus_tag="OQM83_001198"
     CDS             complement(5097..5981)
                     /gene="yhdJ"
                     /locus_tag="OQM83_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCS1519502.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(6067..6363)
                     /gene="fis"
                     /locus_tag="OQM83_001199"
     CDS             complement(6067..6363)
                     /gene="fis"
                     /locus_tag="OQM83_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCS1519503.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(6389..7354)
                     /gene="dusB"
                     /locus_tag="OQM83_001200"
     CDS             complement(6389..7354)
                     /gene="dusB"
                     /locus_tag="OQM83_001200"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCS1519504.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(7683..8564)
                     /gene="prmA"
                     /locus_tag="OQM83_001201"
     CDS             complement(7683..8564)
                     /gene="prmA"
                     /locus_tag="OQM83_001201"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCS1519505.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(8576..10027)
                     /gene="panF"
                     /locus_tag="OQM83_001202"
     CDS             complement(8576..10027)
                     /gene="panF"
                     /locus_tag="OQM83_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCS1519506.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(10017..10259)
                     /locus_tag="OQM83_001203"
     CDS             complement(10017..10259)
                     /locus_tag="OQM83_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCS1519507.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(10368..11717)
                     /gene="accC"
                     /locus_tag="OQM83_001204"
     CDS             complement(10368..11717)
                     /gene="accC"
                     /locus_tag="OQM83_001204"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCS1519508.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     RGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(11728..12198)
                     /gene="accB"
                     /locus_tag="OQM83_001205"
                     /gene_synonym="fabE"
     CDS             complement(11728..12198)
                     /gene="accB"
                     /locus_tag="OQM83_001205"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCS1519509.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(13176..14150)
                     /locus_tag="OQM83_001206"
     CDS             complement(13176..14150)
                     /locus_tag="OQM83_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1519510.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            14302..16242
                     /gene="csrD"
                     /locus_tag="OQM83_001207"
     CDS             14302..16242
                     /gene="csrD"
                     /locus_tag="OQM83_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCS1519511.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAAQLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            16547..17590
                     /gene="mreB"
                     /locus_tag="OQM83_001208"
     CDS             16547..17590
                     /gene="mreB"
                     /locus_tag="OQM83_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCS1519512.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            17656..18759
                     /gene="mreC"
                     /locus_tag="OQM83_001209"
     CDS             17656..18759
                     /gene="mreC"
                     /locus_tag="OQM83_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCS1519513.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            18759..19247
                     /gene="mreD"
                     /locus_tag="OQM83_001210"
     CDS             18759..19247
                     /gene="mreD"
                     /locus_tag="OQM83_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCS1519514.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            19256..19849
                     /gene="yhdE"
                     /locus_tag="OQM83_001211"
     CDS             19256..19849
                     /gene="yhdE"
                     /locus_tag="OQM83_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCS1519515.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            19839..21308
                     /gene="rng"
                     /locus_tag="OQM83_001212"
     CDS             19839..21308
                     /gene="rng"
                     /locus_tag="OQM83_001212"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCS1519516.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            21376..25176
                     /gene="yhdP"
                     /locus_tag="OQM83_001213"
     CDS             21376..25176
                     /gene="yhdP"
                     /locus_tag="OQM83_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCS1519517.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            25332..26777
                     /gene="tldD"
                     /locus_tag="OQM83_001214"
     CDS             25332..26777
                     /gene="tldD"
                     /locus_tag="OQM83_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCS1519518.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(26905..27770)
                     /locus_tag="OQM83_001215"
                     /pseudo
     CDS             complement(26905..27770)
                     /locus_tag="OQM83_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
     gene            27953..28156
                     /locus_tag="OQM83_001216"
     CDS             27953..28156
                     /locus_tag="OQM83_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCS1519519.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            28164..29096
                     /gene="aaeA"
                     /locus_tag="OQM83_001217"
     CDS             28164..29096
                     /gene="aaeA"
                     /locus_tag="OQM83_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCS1519520.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            29102..31069
                     /gene="aaeB"
                     /locus_tag="OQM83_001218"
     CDS             29102..31069
                     /gene="aaeB"
                     /locus_tag="OQM83_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCS1519521.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            31161..31433
                     /locus_tag="OQM83_001219"
     CDS             31161..31433
                     /locus_tag="OQM83_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCS1519522.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(31489..31752)
                     /gene="yhcN"
                     /locus_tag="OQM83_001220"
     CDS             complement(31489..31752)
                     /gene="yhcN"
                     /locus_tag="OQM83_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCS1519523.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(32117..32587)
                     /gene="argR"
                     /locus_tag="OQM83_001221"
     CDS             complement(32117..32587)
                     /gene="argR"
                     /locus_tag="OQM83_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCS1519524.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            33022..33960
                     /gene="mdh"
                     /locus_tag="OQM83_001222"
     CDS             33022..33960
                     /gene="mdh"
                     /locus_tag="OQM83_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCS1519525.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT         8255 a         8454 c         8900 g         8386 t
ORIGIN      
        1 tcctgatacg acatacctgt ccagtcataa ccaatgcccg caggtaattt tgacgcaagg
       61 ttttccatca atgccatggc atcgccggaa ctggttcctg gcgcggcttc cccctgaatc
      121 tccattgacg gcagaccgtt gtagcgttcc agtcgcggag agccatacac ccaatgtgaa
      181 gtggtaaagg cagagaatgg caccatttcg ccgttggcgc tgcggacata aagtttatcg
      241 acatcttctg gcagcatacg gaatttggca tccgcctgaa catacacctt tttcacgcga
      301 ccacggtcga tgaagtcgtt aacgtaagtc ccacccagcg ccgttgaaat ggtctgattg
      361 atgtcagaaa gtgaaacacc taatgcctgc gccttttcct ggtcaacttc cagtttaaac
      421 tgcgcggtgt cttccagacc attaggacgc acgctgacta agctggcagg atgttgcgcc
      481 gccatgccaa gcaactggtt acgggcctgg gtcagggcat cgtgacccag cccagcctga
      541 tcaattaact caaagtcgaa acccgttgcc gtgcccagtt caacaatggc tggcatattg
      601 aatggaatga caaaaccgtc gcggatcttg cccaattcca ttttggcacg atggattacc
      661 gcttccgcac tgttttcgtc accactacgc tcttcccacg gtttcagact gacgaaagcc
      721 ataccggcgt tttgtgcctg gccgctgaag ctaaagccgt taaccgtaaa gacactttct
      781 acgttcgctt tctcgttatt cagatagtaa tccgtcactt gatccaacac tttttgcgtc
      841 cgctcttgcg tcgcgccagc gggtaactga atcatggtca gaaagacacc ctgatcctct
      901 tcaggtaaga aggaagaagg aagacggaag acgtaaaaac aacaccacca ttcctgcaac
      961 aatcagcgca tagatcagta aatatcgtcc tgtggaaccg aggattttgc cgacgctgtt
     1021 ggtgtagtgg ttaacgctat gatcgaaggt ggtattaaac cagccgaaga aaccgccctt
     1081 attttcgtga tgctcagcag agacgggttt aagcagcgtt gcacataacg caggggtaag
     1141 aatcaatgcc accagaacag aaagcgccat tgccgaaacg atggtgatag agaactggcg
     1201 ataaattgcc ccagtagaac cgccgaagaa tgccatcgga ataaataccg ctgacagcac
     1261 catcgcgata cccaccagtg cgccctgaat ttgcgacatc gatttttccg tcgcttcttt
     1321 tggcgggagc ttatcctcca tcatcacgcg ctcgacgttc tccaccacca ctatcgcatc
     1381 atcgacgagc agcccgatgg caagcaccat cccgaacatc gttagtgtgt tgatggagta
     1441 accaaaagcg gcgaggatgg caaacgtccc taacaacacc acgggtaccg caatggtggg
     1501 gatcagcgtt gctcgcatat tctgcaagaa cagatacatc accaggaaca ccagcataat
     1561 ggcttcgaac agcgttttta ccacttcgtg aatagaaagc tggacgaatg gcgtggtgtc
     1621 ataagggtag agaaccttca ttccctgcgg gaagaatggc tgtaattccg ccagttttgc
     1681 cttaatggct ttcgcggtat tgagagcgtt cgcgccggtt gctagcttaa tccccaggcc
     1741 cgccgccggt tttccgttga tacgagcgat aacgttatag ttttcaccgc caagttcaac
     1801 tcgtgcgaca tctttcaggc gtaccaccga gccgtcactg tttacgcgca gggttacttt
     1861 gccgaattct tccggatttt ttaaccgcgt ctgagcaata atcgaggcgt tcaactgttg
     1921 ccctggtaac gctggcgttc cgcccaactg cccggcagcg atctgatcgt tctgtacctt
     1981 caactggtta atcacatcaa ccggtgtcag tttatatttg tttagcagat cggcgtccag
     2041 ccagatacgc atcgcatact gtgcgccgaa aagctgtacg tcaccgacgc cattcagacg
     2101 gctaagcgta tctttaacgt tagaggccac atagtccgag atatcgtcct gtgtggtgtc
     2161 tgggttatca gagacaaagc ccgccaccat caaatagctg ctactggact tttcaacact
     2221 gatcccctgc tgctgaacct cctgcggcag caacggcgtg gcgagctgga gtttgttctg
     2281 cacctgcact tgcgcgatat caggatcggt cccggactgg aaggtaaggg taattgtcac
     2341 gctaccggcg gaatcgctgg tggaggacat atacatcagg ttatcgatac cattcatatt
     2401 ctgttcgata acctgcgtca ccgtatcctg cacggtctgc gcatcagcac ctggatagtt
     2461 tgcctaaaca gaaaccgcag gcggtgcaat ggttggatac tgagcgacgg gcaattgtag
     2521 gatcgccagt gcgcccgcca tcatcagaat aatggccagc acccatgcaa atatcggtcg
     2581 tcgaataaaa aagtttgcca tgtcagatta ccttacttcg atgcagtatc tgcgggggta
     2641 tcggtagtgg ctttaacctg ctctcccgga cgcgcttttt gcaggccgct gacaatgact
     2701 tgatcgccag atttcagacc ttcgctaatc aaccatttat cgccaatcgc ctgactggca
     2761 acgacagggc gcgcttccac ctgactttta tcgttaacaa tcagcacggt tgcatcacca
     2821 cgcggtgtgc ggctaacgcc ttgttgcggg ataagaatgg cgtcaggttg gacgccttca
     2881 tcaatccgtg cacgcacaaa catacccggc aaaagcgtat gttgcgggtt agggaagaca
     2941 gcacgtaggg ttatggagcc ggtgcattca tcaacggtca catcagagaa ttgcagcgta
     3001 cctttcaggg gataggtttg accgttttcc atgaccaact ctacgttgct ggtggcgttt
     3061 tccttatgca aatttccttg ctctacggat tgcttcagcc tcataaaatc gttgctggat
     3121 tgggtcacat caacgtagat aggatcgagc tgctggacag tcgccagttc agtcgtttgc
     3181 ccattagtga caagagcgcc ttcggtcacg gtcgatttgc cgatacgtcc gctaattggc
     3241 gcagtcactt tggtataagc gagattgatg cgagcgcttt cgactgtggc ttttgcggca
     3301 atcacggcgg catcggcctg gcgagcatca gcaatggcct ggtcgtactc ctgctggctg
     3361 atgtatttcg tacccacgag cggaacgtaa cgttttaccg tcaaatgcgc gatggcggcg
     3421 gcggcttcac ttttcgccag ttcgcctttc gcgctgtcat aatttgcctg ataggtcgcg
     3481 ggatcgatct ggtacaggga ctggcctgct tgtacatcgc tgccttcagt gaaattacga
     3541 ttcagtacga tcccgctaac ctgtgggcga acttcggcta tacggtaagc attggtgcgg
     3601 cctggtaatt cagtcttaac ttctaacggg gccgttttca caatatgaac ggtaacctgc
     3661 ggttcaccga cgtgagcttt ctcttctccc ttatcgttac aaccgacgat taaagccgcg
     3721 gagatcagaa taaaggaggg caggaggaaa aacctggcat gtttcgtcat tactattcct
     3781 caaaaaaaca aaagcgcgtt atttacccag aaaggcaacg cgttaactcg cagaaagaaa
     3841 aatacagttt gctatcctac aaattatcat tcgtcgatgt aaggaatagt tatgaatact
     3901 ggcgtctcaa ggcacataaa cacaaaaaaa gattaatatt ctactgtttt attttgacgc
     3961 gggttgaaag aggcagaatt aaaacctcgt aaattgaaat atatattcat gtggcgaatg
     4021 tattttaggt aaataatata tattatttat ttgacgattt atagcaactc aatgttaccc
     4081 tctcgtataa atacacatta gatgatagat taaccttcgc tattttctca ccctgtgtcg
     4141 aatatattta tttcctgaat aattaatcat ggcaaaaaga accaaagccg aagctctgaa
     4201 gacccggcaa gaactgattg aaactgccat cgcccagttt gcgcaacatg gcgtaagcaa
     4261 gacgacgttc aacgacattg ccgatgccgc taacgttacg cgtggcgcta tctactggca
     4321 cttcgagaac aagacccaac tgtttaatga gatgtggttg caacagcctt cattgcggga
     4381 gttaatccag gaacacttga cggctggatt agagcatgac ccgtttcaac aattgcgtga
     4441 aaaattgatt gtcggcttgc aatatattgc caaaattccc cgccagcagg cgttgctgaa
     4501 aatcttatat cacaaatgtg aatttaatga tgggatgctg gccgagggag tgatacgcga
     4561 aaagatgggc tttaatccgc agactctccg cgaagtattg caggcgtgtc agcaacaagg
     4621 ttgtgtagca aataacctcg atttagatgt tgtgatgatt attattgatg gtgccttcag
     4681 cggaattgtt caaaactggt taatgaatat ggcgggttat gatctttata aacaagcacc
     4741 cgcgctggtc gataacgtat taagaatgtt catgccagat gaaaacataa cgaaattaat
     4801 tcatcaaaca aatgaattaa gtgtcatgta aattcacatt ctgtcctgaa aattcagttc
     4861 gtcacagtta accagtttta gtggataaac tgagttcata ttacggcatt tgtctgtttc
     4921 tgttgtcagc agttcaatcc actgcatcag taaagtatca aacagaaaaa cgaagacagc
     4981 gaaaacgacg agccaccaat acttgcgaat cattacctga gcctgtgcat gtttaaccaa
     5041 gtctggcagg aaatatacac gaaatgaata atctgaaaac caggcttaca cttcctttac
     5101 tttgcaatga gatcggggtc aacttcgctt aaccggcttc gttttgacag gttgccggtc
     5161 tttctttttt tcactttcgc cagttcttcc gcggagtaat gcgacgcgac atccagccgt
     5221 cgaagcccca ttttgatgta ctcgctgttg atctcaatac caatgaattt tcgtccgctg
     5281 gcgatggcta cggcaccggt agtaaagcta ccggcaaacg ggtcgagaac gatatcgcct
     5341 gggttggaag aggcgagaat aatgcgtttc agtaaggctt ccggtttttg cgtcgggtgg
     5401 ttctcatatt catccatcaa ataacgcacg cgcggaaaat cccagacgtt acccggtact
     5461 ttttgatgat tgtatggctg tggaggattt ttgcgataat cgatcaacgc gcgctgcgat
     5521 ccggttttgg cttctaccag aatagcatca ccgttgaatg tgtagttctt tgcgtctttc
     5581 accatcatca ggatgggttc gtacatggag ccgtagtgtt ttttcgcctg tactccagaa
     5641 ctgtcatatg accagacgat gcgacttttg atggtaaaaa gcttgcggca ctggagatcg
     5701 ataaagggca tgttttccgt actgttcata atgtacatgc tgccctgctt tttcagaacg
     5761 cggtggcact ctacaatcac ttcaaacagc cagtcgataa acagatcttc tttccaggct
     5821 tcgatcagac catcaaaatt tttaccgatg ttatacggtg ggtcggcaaa gatcagatcg
     5881 acgctttcag taggtagctt tttaagttcg gcaagggcat caccgtgaat aatggtctta
     5941 gcttcattac caaaccgggt cggttcacat cctgttctca tggtcactcc ctttgtgaca
     6001 cctataaaaa aggcgcttcc ccatgccgag tagcgccttt ttaatcaagc atttagctaa
     6061 cctgaattag ttcatgccgt attttttcaa ttttttacgc agcgtaccac ggttgatgcc
     6121 catcatcagc gcagcacggg tctggttacc acgggtgtat tgcatcacca tgtccaacag
     6181 gggctgttct acttcagcca gtaccagctc atagaggtca ttcacatcct gaccattcag
     6241 ttgagcaaaa tagttcttca gtgcctgttt aaccgagtca cgcaggggtt tttgggttac
     6301 ctgatcctga gagttaacgg tagaaacggt cagtacgtca gaatttacgc gttgttcgaa
     6361 catagttctg tcagctcttt atttctgttt acgcaaaatt ttcgaagtat gcctccaacg
     6421 cctccagctg ttcgctggca tcctcaatgg cgttgaatgt gcgccgaaac tggtcatttg
     6481 gagcgtgttc ctggagatac caggaaacgt gtttacgtgc aattcggtac ccttttgccg
     6541 gaccataaaa gtcatgcagt tcccgaacgt gcgcgcaaag caagcgctta acctctgcca
     6601 aaggcagcgg gggcagcaac tccccagtgt ccagataatg ctggatttcc cgaaagatcc
     6661 agggtcttcc ctgagctgcg cggcctatca tcagggcatc cgcccctgta tagtcgagca
     6721 cagctctggc tttaagcggg tcagtaatgt cgccattcgc gataaccgga atggaaactt
     6781 tctgcttaac tgcccgaata ctgtcgtact cagcttctcc attgaacaaa caggcgcgtg
     6841 tacggccatg aatggtcaga gcctgaatgc cacagtcttc agccagttgg gcaatctctt
     6901 cgcagttacg gtgttccggt gcccagccgg tgcgaatctt cagggtaaca ggaacgtcca
     6961 ctgcattgac gacctcggta aggatcgatt taacgacatc cgggtactgc aagagggctg
     7021 aacctgcgag cttgcgattc acttttttag ccgggcaacc catattgata tcaataatct
     7081 gggcaccgct ttccacgtta atacgtgctg catctgccat ttctttcgga tcgctaccag
     7141 caatttgcac ggtgcgaata ccgggttcat caatgtgcac catccgtaaa cgagatttgt
     7201 cgctttccca aacctgtggg ttagaagaca tcatctcgga tactgtcaat ccggctccca
     7261 tctcgtagca caacgtccga aaaggtctgt ctgtaatgcc agccatgggc gctgcgatca
     7321 ggcgatttct gagctgatat tgtccgatgc gcatgagtta agaaatgacc atactgtgac
     7381 tgcaaggcgg cgtatattac gcattttttg cacgagatga aaggccaaac tttgaccaat
     7441 cctctgagat ggatcaaaga attgcattta aaatgagcgt ggtgagataa ttactcataa
     7501 aaatcatcat attagaaaat agtgactaaa atttacactc aaagaaattt gagtaagttc
     7561 tcaatttttc tttatgaatg aaaattttgg cacgcaaatt tgcgtaaata accggcaaat
     7621 ttacgtgcct tttgtgagct tgctcgcact tcgccccgcg tcaccctacg gcgatgcgaa
     7681 ggttaattct tacgaccggt aatgcggcac cactcttctt tttccacgac cgggtccagt
     7741 gcgaagctgt cgacataagc ttcacaaacg ctctctgcct ggcttgccag aataccggaa
     7801 aggcccagca aaccgcctga aaccggcagg acgctgatta acggtgccag ttcacgtaat
     7861 gggcctgcaa ggatgttagc gaccaccacg tcggctttca tttcttctgg ctgatctttc
     7921 ggtaagtaga gttccagacg gtcagaaacg ccattacgtt cggcgttgtc gcggctagcc
     7981 tgaatcgcct gcggatcgat atcaatacca atggcttttg ctgcacccag tttcagcgcc
     8041 gcgatagcca gaatgccgga accacagcca aagtcgatga ctattttacc ggttaaatcg
     8101 aggctgtcga gccactgcag gcacagagag gtagttggat gggtacccgt accaaacgcc
     8161 agccctggat ctaacatcac gttgacggcg ttttcgtccg gcacatcacg ccagctaggg
     8221 cagatccaca gtcgttcacc aaagcgcatc gggtggaaat tatccatcca ttcgcgctcc
     8281 cagtctttat cttccagttg ttcgatttta tgcgcgaagc ctgcgccgag cagcggatgg
     8341 ttttccagaa tcgccaccac gtcgttcata tcggtttcag cgtcgaacag accaatcaca
     8401 tcggtgtcgc cccacaggcg cgtttcgccc ggcagcggct caaatactgg cgtatcgtgg
     8461 gtatcctgaa aagtgataga aacggcaccc gcttccatca gcgcatcgct aagatcttca
     8521 gcgttcgcgc cggtggtgtt cagtttcagt tggatccaag gcatggcaaa actctttatt
     8581 tatcagtagt caaaacggta gcttgcggga cggatgtacc gaaacggttt ccgaccagga
     8641 aagccagcaa acttagtagt aacgagggca cgatagggtg gaagcccagg tactgaatat
     8701 tcagcgtcgc gagtacggca tacagcacgc cgccaacgat catcgcactt agcgcgcctt
     8761 tggcgttggc gcgttcccag taaagaccca gcaccagcgg ccacaggaaa acggcttcca
     8821 gcccaccgaa ggccagcaaa ttcagccaga tgatcatttc tggcggcttc caggcggcaa
     8881 gcagcagcaa cgcgccgaga actaacgtaa ttaccgccga catccgcttc agacgcgtct
     8941 cgttttgcat ttgatccgga cggatattca gatagagatc tttaatgatc gtagcggaac
     9001 tttgtagcaa ttgggcgtta attgtcgaca tgatcgcagc cataggtgca gccagaaaga
     9061 tcccggcagc aaacggtggc agcactttta ccattaacgt cgggatcacc agatccggta
     9121 cggtgagatc ggggatcact gcacgaccta acgctccggc taggtgcata ccgaacatca
     9181 ggattgcgac tacaatcgta ccgatgatga tcccccggtg taccgctttg ctgtctttat
     9241 aagagataca gcgcaccgca gtatgcggca ggccaatcac gccaaagcac accagtaccc
     9301 agaacgacgt cataaaggca ggcgacagaa tatcgtcagc gccttgtggc gtaaccagtt
     9361 gcggatcgat ggtttgcaag gtctgtactg cgttgcttaa gccgccagcg gcatgtacca
     9421 cgccaataag cagcacaacg gtgccaatca gcatcacaag cccttgcatg gtgtcgttca
     9481 gcacgctggc gcgaaagccg ccaaaggcgg tatacaacgc aatgctgata ccgaaaatca
     9541 gcagcccggt ttcataagga atacccgccg cggtttccag caggcgcgca ccgccgataa
     9601 actgcacggt cattgcccca acgaacgcaa ccagcaaact caaactcgcc agccacacca
     9661 gaagacgact ctggtagcgg gcaaacagca tgtcgttcag cgtcactgca ttgtagcggc
     9721 gcgcaagaat cgcaaacttc ttgccgagaa taccgagtga aagccagact gcaggaagct
     9781 gaatcatcgc cagcaatacc cagcccagcc cgtatttata agcagctcct ggcccgccga
     9841 taaacgaact ggcactgata taggtcgcgg tgagcgtcat cgccagcaca ataccgccca
     9901 tagagcggct gccgaggaaa tactcattaa ggaaggtgcc ggtgctccgt ttacgcatcg
     9961 cataaaccga gataccgaac accaccacca gataggcgac cagcggtaga attacttcaa
    10021 gctgcatcgt catcctccag tgggatatcg cgatagataa atttcaccat cgcccagcac
    10081 agtccaataa acagtagcgg cgtcaggatg caggccatct caaaccagcg cggaaagccg
    10141 gtaaaaccgg gggcaacgcc aggtaagtaa gcggctacta accaaactgc cagatacaaa
    10201 agggtcagcc ccagcgccca gcgcgcctct ttatgggcct gaacaaaacg agtgtccatt
    10261 ttttgtccct gatgggtgaa gaaagcgagg attgtacctt atgggggttg tcgatcccca
    10321 gtaataaaaa aggccggaaa atccggcctt ttggcgcttt tgcagtctta tttttcctga
    10381 agaccgagtt ttttctccag atagtggatg ttagtgccac catgctggaa gttctcgtca
    10441 ttcatgatgc ggatctgcag atcaacgttg gttttgatac cgtcgatgat cagctcctgc
    10501 agcgcattct tcatgcgggc aatcgccacg tcacggtttt caccgtagca aatcagctta
    10561 ccgatcattg agtcatagta cggcggtacg gtgtagcccg cgtagatatg agactcccaa
    10621 cgtacgccaa aaccgccagg tgcgtggaaa cgggtgattt tgcctgggct tggcaggaag
    10681 gtgttcggat cttcggcgtt gatacgacat tccaccgcat ggccgcgaac gtgaacttct
    10741 tcttgcttga tcgacagcgg ttgaccggca gcgatacgga gctgttcttt gatcaggtca
    10801 acgccagtga tcatttcggt aaccgggtgt tctacctgaa tacgggtgtt catttcgatg
    10861 aaatagaact cgccgttttc gaacagaaac tcgaaagtac ctgcaccgcg atagccgata
    10921 tcaacacacg ctttagcgca acgttcgccg atgtagcgac gcagttccgg ggtaatgccc
    10981 ggtgctggcg cttcttcgac cactttctgg tggcggcgct gcatggagca gtcacgttcc
    11041 gccagataga tagcgttgcc ccgaccgtca gccagtacct gaatctcgac gtggcgagga
    11101 ttttccaggt atttctccat gtaaaccata tcgttgctga aagcagcttt cgcttccgca
    11161 cgggtcatgg agatggattg tgccagttca gcgtcgccgc gcactacgcg cataccgcga
    11221 ccaccgccgc cgccggaggc tttgataatc accggataac caatgcgttt agcaatggca
    11281 cggtttttat ccatatcgtc gcccaacggg ccgtcagaac ccggtacgca agggacgccc
    11341 gcttttttca tcgccgcgat tgcggatact ttgtcgccca tcaggcgaat ggtttctgct
    11401 ttcgggccga tgaagataaa gccggagcgt tcaacctgct cggcaaagtt ggcgttctcg
    11461 gagaggaagc cgtaacccgg atggattgct actgcgccgg tgatttcagc ggcgctgatg
    11521 attgccggga tgttcagata actttttact gacggagcag ggccaataca gaccgtttca
    11581 tctgccagta atacgtgttt tagatcgcga tccgcgctgg agtgcacagc gacagtcttg
    11641 atgcccagtt ctttacaggc acgaagaata cgcaatgcaa tctcgccgcg gttggcaata
    11701 acaattttat ccagcatgtt cgcctcgtta ctcgatgacg accagcggct cgtcaaattc
    11761 taccggttgt ccactttcga ccagaattgc tttcacggta ccggatttgt ccgcttcgat
    11821 ctggttcatc attttcatgg cttcaacgat gcacagggta tcgcccacgt tgactttctg
    11881 acccacttcg atgaacgctt ttgcgtccgg gcttggggtg cggtagaaag taccaaccat
    11941 cggggaacgt acgatgtgac cactgatttc cgctgctgct ggcgcttcca tggaaggaac
    12001 ggtcgccgga gcggctgcgt tagattgagc tggctgctgc atcattggtg cagcgtaagc
    12061 ttgttgcatc acagggaaac ttgcggcagg agctgcacgg ctaatgcgta ctgactcttc
    12121 gccttcagaa atttccagtt cggagatgcc tgattcttca accagctcga tcagtttttt
    12181 aatcttacga atatccatga gtgggttccg tactctttgt ttagtgtgat tgtgacaggc
    12241 gtttcaccgc cgtctgtaaa gcgtatgccc cgacagctgt atgcatagcg ataaattcca
    12301 gcaggccggg acgccggtct attttgcccc agagtatcaa tgttagactt gacggacatt
    12361 gtgcagcgat ctgctgctat cctccggcaa aaaacaaaat ataccttcgt tgcgccaatg
    12421 tcaccttttc cgcagcgcaa aaactgcgtc agggaggacg aggtcgcaca ttataacgat
    12481 ttcgtagcaa ttggcagcta aatactggtc tcatcaggga agataatcaa cagctaacat
    12541 gtaaataacc ttcaacaccg tgtaatttgc aacaagccgc acaattcacg aaattagcgc
    12601 catcatcgac ggaacttctt ataacgtaag gtgaaaagct gtaaagccag ccctgcgaag
    12661 atgaggggct gcaatgagat aatcttcaca gaccacaaat aatgtaggga tgccaggatc
    12721 gcgacaagat agacgacatt acgcagcggt tgctggggat tacccaattt tcactgcatc
    12781 gtctggatag atgtgaatgc taaagcacgc aaaatcaacc gactgacaat tctcagcatt
    12841 aaataggttc gagtggttaa tttgtagtca aataaaatca gattattcac cccatggtac
    12901 cagaaatcag taacaggtta agcgtagtgt cgcccaggca aagcaccata aacctaacag
    12961 gcgacggagg cgtatcaata atggctgttt agaggagtat caatgcgcat caatttgtgg
    13021 tcagtcgaaa tcatgacttt ccagtttgga tgatgttgta caacttcctg accaaggtgt
    13081 tcgacgaaag ctgtccggct aaccgctttt cccgttaacc gattgaataa tgatgtcaaa
    13141 atgagttccc tctcttatta ttccttgcta aatggttagt taaccttcac cagcgtgcga
    13201 ccctggatct ggttattaat gatggcctcg gcaaagttcg gtgcctctga cagagatatc
    13261 tcttttgccg cctgggtata gaatgattcc ggtaaatcgg cgaccagtcg ctgccaggct
    13321 tgtgcgcggc gttctggtgg cgtcattact gaatccaccc cttgcaaacg gacattacgc
    13381 agaataaatg gcatgaccgt ggttggcaga gtaaaaccac ccgccagacc acaggccgcc
    13441 acgcagccgc cgtaattcat ttgcgccagc acttttgcca gcactttgtc gccaacagtg
    13501 tcaattgccc cagcccagac ttgtttttcc agaggacggg attcggcaaa ctcatcacga
    13561 gggagaatac ggctggcacc taaacttttc agatattcat gggtactttc gcgaccggaa
    13621 acggcaacga cctgataacc caacttatgc agcagcgcca cggcggtact gccgacgcca
    13681 ccactggcac ccgtcacgac aatctccccg tcctgcgggc gaacaccggc atcttccagc
    13741 gccatcacac acagcatggc ggtaaaaccg gcagtaccga taatcattgc tttacgcgcg
    13801 tccagccctt gcggcatggc aaccagccag tcacctttca ctcgcgcctg ctccgccagc
    13861 ccaccccagt ggttttcacc aacgccccag ccagtgagta acacctcctg accggcatga
    13921 aaacgcggat cttcgctggt gcgtacagtt ccggcaaaat cgatcccagg aatcatcgga
    13981 aaattacgga tgatttttcc cttaccggta atcgccagcg catctttata gttcaggctc
    14041 gaccagtgaa catcgaccgt gacatcgccc tccggcaggc gactttcgtc cagagtctgt
    14101 actgatgcga gagttttgcc gtcctgctgt tctaaaagta acgcctgcat aagtggtcct
    14161 catgtgcatg atggattgga aaataatttc tgaagattat actcgctaat ggaaaagcaa
    14221 agccgatgaa gcgcaataaa ttgcgagata aatctgattt gctagtatgc ccgcttcctc
    14281 actatcggag ttaacacaag gatgagatta acgacgaaat tttcggcctt tgttacgctg
    14341 ctcaccgggt taacaatttt tgtgactttg ctgggctgtt cgctaagttt ctacaacgca
    14401 attcagtata agtttagtca tcgcgttcag gcggtggcga cggcgattga tacccacctt
    14461 gtgtcgaatg acttcagcac attaaggcca caaattaccg aattaatgat gtcggcagat
    14521 atcgttcgtg tagacctgct ccatggtgat aaacaggttt ataccctggc cagaaatggt
    14581 agttatcgtc cggttggctc cagcgatctg tttcgcgaac tgagtgttcc gttgataaag
    14641 catccgggga tgtcgctgcg tctggtttat caggatccga tgggcaacta tttccattct
    14701 ttaatgacta ccgcgccgct cacgggggcg attggcttta tcattgttat gctcttcctg
    14761 gcggtacgct ggttacaacg gcaacttgcc gggcaagaat tgctggaaac ccgggctact
    14821 cgtatcttaa acggtgagcg tggctctaat gtgttgggaa ccatctatga atggccgccc
    14881 agaaccagca gtgcgctaga tacgctgctt cgtgaaattc agaacgcacg cgaacaacac
    14941 agccgtcttg atacgctgat ccgctcttat gccgcccagg acgtgaaaac cggcctcaat
    15001 aaccgactct ttttcgataa tcagttagca acgttactgg aagatcagga gaaagtaggt
    15061 acccacggga tcgtgatgat gattcgtctg ccggatttca atatgttgag cgacacttgg
    15121 gggcacagcc aggttgaaga acagttcttc actctgacta atctgctgtc gacatttatg
    15181 atgcgctacc ctggcgcact gctggcgcgt taccaccgca gtgattttgc tgcgcagtta
    15241 ccgcaccgga cgttaaaaga ggcagagagc atcgccggtc agttaatcaa agccgtcgat
    15301 accttgccga acaataaaat gctcgatcgc gacgatatga tccacattgg tatctgcgcc
    15361 tggcgtagtg gtcaggatac cgagcaggta atggaacatg cagagtctgc cacgcgtaat
    15421 gcgggattgc agggcggcaa tagctgggct atttacgatg actcgttgcc tgaaaaagga
    15481 cgcggtaatg ttcgctggcg tacgcttatc gagcaaatgc tcagtcgcgg cggcccgcgc
    15541 ctttatcaaa aaccggcggt tactcgcgaa ggtcaggttc atcatcgcga actcatgtgc
    15601 cgcatcttcg atggtaatga agaggttagc tcggcggagt atatgccgat ggtcttgcag
    15661 tttggcttat cggaagagta tgaccgtctg caaatcagcc gtcttattcc actattgcgt
    15721 tactggccag aggaaaatct ggcgattcag gttaccgttg agtcgctgat tcgcccgcgt
    15781 tttcagcgtt ggctgcgcga tacgttaatg caatgtgaaa aatcacaacg aaaacgcata
    15841 attattgaac ttgcagaggc cgatgtaggt caacatatca gtcgtttaca acctgttatt
    15901 cgtttagtga atgctttagg ggtacgggta gccgtcaacc aggctggttt gacgctggta
    15961 agcaccagtt ggatcaaaga acttaatgtt gagttactca agctccatcc ggggctggtc
    16021 agaaacattg agaagcgaac ggagaaccag ctgctggttc aaagcctggt ggaagcctgc
    16081 tccgggacca gcacccacgt ttacgccacc ggcgtgcgtt cgcgaagcga gtggcagacc
    16141 ctgattcagc gcggtgttac aggcgggcaa ggggattttt tcgcgtcctc acagccactt
    16201 gatactaacg tgaaaaaata ttcacaaaga tactcggttt aacctgccgt ttaatccgtt
    16261 ttcacgtaga ataacgcgcg ctgcgtctca tgggagtgtg cttgtctgct cgccagattg
    16321 ttgcagcaca tatgcagatg aatgacctta cgcggttgca aacaggcgag gaatgctgct
    16381 gatgcattaa gcctttctgg actcaggcag agatttgtaa caaaggaaac gaactgcact
    16441 aattttcacc gtagcagatg atttttgcgc cttgtcgctg ctgcgtgtgg ttggtaaagt
    16501 aagcggattt tcttttccgc cccagctttc aggattatcc cttagtatgt tgaaaaaatt
    16561 tcgtggcatg ttttccaatg acttgtccat tgacctgggt actgcgaata ccctcattta
    16621 tgtaaaagga caaggcatcg tattgaatga gccttccgtg gtggccattc gtcaggatcg
    16681 tgccggttca ccgaaaagcg tagctgcagt aggtcatgac gcgaagcaga tgctgggccg
    16741 tacgccgggc aatattgctg ccattcgccc aatgaaagac ggcgttatcg ccgacttctt
    16801 cgtgactgaa aaaatgctcc agcacttcat caaacaagtg cacagcaaca gctttatgcg
    16861 tccaagcccg cgcgttctgg tttgtgtacc ggttggcgcg acccaggttg aacgccgcgc
    16921 aattcgtgaa tccgcgcagg gcgctggtgc ccgtgaagtc ttcctgattg aagaaccgat
    16981 ggctgccgca attggtgctg gcctgccggt ttctgaagcg accggttcta tggtggttga
    17041 tatcggtggt ggtaccactg aagttgctgt tatctccttg aacggcgtgg tttactcctc
    17101 ttccgtgcgc attggtggtg atcgtttcga cgaagctatc atcaactatg tgcgtcgtaa
    17161 ttacggttct ctgatcggtg aagccaccgc agaacgtatc aagcacgaaa tcggttcggc
    17221 ttacccgggc gatgaagtcc gtgaaatcga agttcgtggc cgtaacctgg cagaaggtgt
    17281 tccacgcggt tttaccctga actccaacga aatcctcgaa gcactgcagg aaccgctgac
    17341 cggtattgtg agcgcggtaa tggttgcact ggaacagtgc ccgccggaac tggcttccga
    17401 catctccgag cgcggcatgg tgctcaccgg tggtggcgca ctgctgcgta accttgaccg
    17461 tttgttaatg gaagaaaccg gcattccagt agttgttgct gaagacccgc tgacctgtgt
    17521 ggcgcgcggt ggcggcaaag cgctggaaat gatcgacatg cacggcggcg acctgttcag
    17581 cgaagagtaa tcggatgcag gcaggggaaa tgtctgttta ccctgcctgg tctgatacga
    17641 gaatacgcat aacttatgaa gccaattttt agccgtggcc cgtcgctaca gattcgcctt
    17701 attctggcgg tgctggtggc gctcggcatt attattgccg acagccgcct ggggacgttc
    17761 agtcaaatcc gtacttatat ggataccgcc gtcagtcctt tctactttgt ttccaatgct
    17821 cctcgtgaat tgctggatgg cgtatcgcag acgctggcct cgcgtgacca actagaactt
    17881 gaaaaccggg cgttacgtca ggaactgttg ctgaaaaaca gtgaactgct gatgcttgga
    17941 caatacaaac aggagaacgc gcgtctgcgc gagctgctgg gttccccgct gcgtcaggat
    18001 gagcagaaaa tggtgactca ggttatctcc acggttaacg atccttatag cgatcaagtt
    18061 gttatcgata aaggtagcgt taatggcgtt tatgaaggcc agccggtcat cagcgacaaa
    18121 ggtgttgttg gtcaggtggt ggccgtcgct aaactgacca gtcgcgtgct gctgatttgt
    18181 gatgcgaccc acgcgctgcc aatccaggtg ctgcgcaacg atatccgcgt aattgcagcc
    18241 ggtaacggtt gtacggatga tttgcagctt gagcatctgc cagcgaatac ggatattcgt
    18301 gttggtgatg tgctggtgac ctccggtctg ggcggtcgtt tcccggaagg ctatccggtc
    18361 gcggttgtct cttccgtaaa actcgatacc cagcgcgctt atactgtgat tcaggcgcgt
    18421 ccgactgcag gactgcaacg tttgcgttat ctgctgctgc tgtggggcgc ggatcgtaac
    18481 ggcgctaacc cgatgacgcc ggaagaggtg catcgtgttg ctaatgaacg tctgatgcag
    18541 atgatgccgc aggtattgcc ttcgccagac gcgatggggc caaagttacc tgaaccggca
    18601 acggggatcg ctcagccgac tccgcagcaa ccgacgacag gaaatgcagc tactgcgcct
    18661 gctgcgccca cacagcctgc tgctaatcgc tctccacaaa gggctacgcc gccgcaaagt
    18721 ggtgctcaac cgcctgcgcg tgcgccggga gggcaatagt ggcgagctat cgtagccagg
    18781 gacgttgggt aatctggctc tctttcctca ttgcgctgtt gctgcaaatc atgccctggc
    18841 cggataacct gattgttttc cggccaaact gggtgttact catcttgttg tattggatcc
    18901 tggccttgcc tcatcgcgta aatgtgggca caggttttgt gatgggtgcc atactggatc
    18961 tgatcagcgg ctcgacgctt ggcgtacgcg tattggcgat gagcatcatt gcttacctgg
    19021 tggcgctgaa ataccagctt ttccgcaacc tcgcattatg gcagcaggcg ctggtcgtca
    19081 tgttgctttc gctggtggtg gatattattg ttttctgggc agagttttta gtgattaacg
    19141 tctctttcag accggaagtg ttctggagta gtgtagtcaa tggggtgctc tggccgtgga
    19201 ttttcttgct gatgcgtaaa gtccgtcagc agtttgcagt gcaataaagg tttctatgac
    19261 ttctctgtat ttagcttccg gttctccgcg tcgtcaggag ttacttgcgc aacttggtgt
    19321 gacctttgaa cgtattgtta cgggcattga ggagcagcgt cagccgcagg agagcgcgca
    19381 gcagtatgtt gtgcgtctgg cgcgcgagaa agcacaggcg ggtgtcgcgc aaacggcgca
    19441 ggatctcccg gtgctgggtg cggatactat cgttatcctg aatggagaag tgctggagaa
    19501 accgcgcgac gcagagcatg cggcgcagat gttgcgcaaa ttatcgggtc agacccatca
    19561 ggtgatgacg gcagtggcgt tggccgacag ccagcacatt ctcgattgcc tggtggtcac
    19621 cgatgtgact ttcagaacgt taacagacga agacatcgcg ggctatgtcg ccagcggtga
    19681 accgttagat aaagcaggtg catacggtat tcaggggctg ggtggctgtt ttgtcaggaa
    19741 gataaatggc agctatcacg ccgtagtcgg cttacctctg gttgaaacgt atgaattatt
    19801 aagtaatttt aacgcactgc gtgagaaaag ggataaacat gacggctgaa ttgttagtaa
    19861 acgtaacgcc ttcggaaacg cgagtggcgt atattgatgg cggtattctg caggaaattc
    19921 atattgaacg tgaggcgcga cgcggaatag taggcaatat ctacaagggt cgtgtaagtc
    19981 gtgtacttcc gggtatgcag gcggcttttg tagatattgg gctggataaa gccgcgtttc
    20041 ttcatgcatc cgatatcatg ccgcacaccg aatgtgtggc gggtgaagaa caaaagcaat
    20101 tcacggtgcg cgacatctcg gaactggttc gtcaggggca agatctgatg gtgcaggtgg
    20161 tgaaagatcc gcttggcact aaaggtgcgc gcctgaccac cgatatcacg ctgccttctc
    20221 gctatctggt ctttatgcca ggggcttctc acgttggggt ttcccaacgt attgaaagcg
    20281 aatcagaacg tgaacgcctg aaaaaagtgg tcgcagagta ttgcgacgag cagggcgggt
    20341 ttatcatccg taccgcagcg gaaggggttg gcgaggctga actggcctcc gatgccgctt
    20401 atctgaaacg cgtctggacc aaagttatgg agcgtaaaaa gcgcccgcag acccgttatc
    20461 agctgtacgg cgaactggcg ctggctcagc gtgttctgcg tgatttcgcc gacgccgaac
    20521 tggaccgcat tcgcgttgac tcacgcctga cttacgaagc attacttgag ttcacctcgg
    20581 agtacattcc cgagatgacc agcaagctgg agcattacac cggacgccag ccgattttcg
    20641 atctctttga tgtcgaaaac gaaatccagc gagcgctgga acgcaaagta gaactgaaat
    20701 ccggtggtta tctcattatc gaccagaccg aagcgatgac caccgtggac atcaataccg
    20761 gagcgtttgt cggtcatcgc aatctggacg acaccatttt caataccaat attgaagcga
    20821 cgcaggctat cgctcgccag ttacggttgc gtaatctggg cgggattatc attattgatt
    20881 tcatcgatat gaataatgaa gatcaccgcc gccgcgtgct gcactcgctg gagcaggcgt
    20941 tgagcaaaga ccgggtgaaa accagcgtta atggtttttc ggcgctgggg ctggtggaga
    21001 tgacgcgtaa acgcacccgc gaaagcattg agcacgtact gtgtaacgaa tgcccaacct
    21061 gccacggtcg cggaacggtg aaaaccgtgg aaacggtatg ctatgaaatc atgcgcgaga
    21121 ttgttcgtgt ccaccatgct tacgactccg accgtttcct ggtctatgct tctccggcag
    21181 tagccgaagc cttgaaaggc gaagagtcac actcgctggc ggaagtggaa attttcgttg
    21241 gcaaacaggt taaagtacaa attgaaccgc tctataacca ggagcagttt gacgtcgtaa
    21301 tgatgtaaac agatgctggg ccgccatccg gcaaagggtt tttgagtcac atttttagca
    21361 gacaaggagt gacgggtgag gcgattgccg gggattttac tgcttactgg agccgcgctc
    21421 gttgtgatcg ctgccctgct ggttagcggc ctgcgtattg ctttaccgca tcttgacgcc
    21481 tggcgtccgg aaatcctcaa caaaatagaa tccgcgactg gcatgccggt agaagccagt
    21541 cagctctcag ccagctggca gaattttggc ccgacgcttg aagcacacga catccgtgca
    21601 gaactaaaag atggcggcga attttcggtt aaacgcgtta ctctggcgct ggatgtctgg
    21661 cagagcctgt tacatatgcg ctggcagttt cgcgacctca ctttctggca gctgcgcttt
    21721 cgcaccaaca ctcctatcac cagcggtggt agcgatgaca gtctggaagc cagtcacatc
    21781 agcgatctgt ttcttcgtca atttgaccat ttcgatctcc gcgacagtga agtcagtttc
    21841 ctgacgccat ccggtcagcg tgccgagctg gcgatcccac aactcacctg gctgaacgat
    21901 ccacgtcgac accgtgcgga aggcctggta agcctctcca gccttaccgg acagcacggc
    21961 gtgatgcagg tgcgcatgga tttgcgcgat gatgaggggt tgttaagcaa tggtcgcgtc
    22021 tggctacagg cggatgacat cgacctgaag ccgtggctcg gtaaatggat gcaggacaat
    22081 attgctctgg aaacggcaca gttctccctt gaaggctgga tgacgatcga caaaggcgat
    22141 gtaaccggcg gtgacgtctg gctgaaacag ggcggtgcca gctggttggg cgagaagcaa
    22201 acgcatacgc tgtcggtgga taatctgacc gcgcatatta cgcgtgaaaa tccgggctgg
    22261 cagttctcta ttcccgatac acggatcacg atggacggca aaccctggcc gagcggagca
    22321 ttgacgctgg cctggatacc ggaacaggac gttggcggca aagacaataa acgcagtgac
    22381 gaactccgga ttcgcgccag taatctggag ctggcaggcc tggagggcat acgcccgctg
    22441 gccgcgaaac tttcacctgc actgggtgat gtttggcgct ccacacaacc gagcggcaag
    22501 attaacactc tggcgctgga tatcccgctt caggcggcag acaagacccg ttttcaggca
    22561 tcgtggagcg atctggcctg gaagcaatgg aaattattac cgggtgcgga acacttctcc
    22621 gggacgcttt ccggcagcgt tgaaaatggt ttgcttaccg cgtcgatgaa gcaggcaaag
    22681 atgccttacg aaacggtatt ccgtgcgcca ctggaaatcg ccgacggcca ggcaactata
    22741 agctggctga acaatgacaa aggtttccag ctggatgggc gtaatattga cgttaaagcc
    22801 aaagccgtcc atgcgcgcgg cggttttcgt tacctgcaac ctgctaacga tgaaccctgg
    22861 ctgggtattc tggctggcat cagtaccgat gatggttcac aagcctggca ctatttcccg
    22921 gaaaacttga tgggtaaaga cctggttgat tacttaagtg gcgcgattca gggcggtgaa
    22981 gcggataacg cgacgctggt ttatggtggc aatccgcaac tcttccccta taaacacaac
    23041 gaaggtcagt ttgaagtgct ggtgccgctg cgcaacgcga agtttgcctt ccagccggac
    23101 tggcctgcat taactaacct tgatattgaa ctggacttta ttaacgacgg tttatggatg
    23161 aaaaccgatg gcgttaatct gggcggcgtg cgcgcgagta atctcaccgc agtgatccct
    23221 gactactcga aagaaaaact gctgattgac gctgacatta aaggtccggg taaagccgtt
    23281 ggcccttact ttgatgagac accgctgaaa gattctctgg gtgcgaccct gcaagaactc
    23341 cagctcgacg gcgatgtgaa tgctcgctta catcttgata tcccgctgaa cggcgagctg
    23401 gtaaccgcga aaggtgaagt gacgctgcgt aataacagtc tgtttatcaa accactcgac
    23461 agcaccctga aaaatttgag cggtaaattc agctttatca atggcgatct gcaaagtgaa
    23521 ccactgacag caagctggtt taatcagccg ttgaacgtgg atttttccac caaagaaggg
    23581 gcaaaagcct accaggttgc ggtgaatctc aacggtaact ggcaaccggc gaaaaccggc
    23641 gttctgcctg aagcggtgaa cgaagcattg agtggcagcg tggcgtggga tggtaaagtg
    23701 ggcattgatc tgccttatca tgctggcgcg acctataacg tagagctgaa cggcgatctg
    23761 aataatgtga gtagtcactt accttcaccg ttagccaaac ctgcgggtga accactagcg
    23821 gtaaacgtta aggttgatgg caatctcaac agctttgaat taaccggaca ggctggtgcg
    23881 gataaccatt tcaatagccg ctggttgctt ggtcaaaagc tgacgctcga tcgtgctatt
    23941 tgggcggcag acagtaaaac gctcccgccg ttgccggaac aaagtggcgt tgaactcaat
    24001 atgccgccga tgaatggtgc cgagtggctg gccctgttcc agaaaggcgc tgcggagagt
    24061 gtcggtggtg cagcgagttt cccacaatac ataacgttac gtacgcctat gttgtcactg
    24121 ggaaatcagc aatggaataa cctgagtatt gtttcgcaac cgacggcaaa tggcaccctg
    24181 gttgaggcgc aagggcgtga aatcaacgcc acgctagcga tgcgtaataa cgcgccgtgg
    24241 ctggcgaata tcaaatatct ttattacaac ccgagcgtgg cgaaaactcg tggtgattca
    24301 acgccgtcat cacctttccc gacaacggag cgcattaact tccgtggctg gccggacgca
    24361 caaatacgat gcacagagtg ctggttctgg gggcaaaaat tcggtcgcat tgacagtgat
    24421 atcaccattt ctggcgatac gttaacgctg accaatggac tgattgatac tggtttctcg
    24481 cggcttactg ccgatggtga atgggttaat aatccgggga atgaacgtac ctcgctgaaa
    24541 ggaaaactgc gcgggcagaa aattgatgcc gccgcagaat tttttggtgt cacgacgccc
    24601 atacgccagt cgtcatttaa tgtggattac gatttacact ggcgcaaagc accctggcag
    24661 ccagatgaag cgacgttgaa tggcatcatt catactcaac tgggtaaagg cgaaattacc
    24721 gaaatcaata ccggacatgc cgggcaattg ctgcgcttat tgagcgtaga tgccctgatg
    24781 cgtaagctgc gttttgattt cagagacact tttggcgaag ggttctattt tgactccatt
    24841 cgcagcaccg cgtggattaa agacggggtt atgcacaccg acgacacgct ggtggatggc
    24901 ctggaggcgg atatcgccat gaaagggtcg gtaaatctgg tacgtcgcga cctgaatatg
    24961 gaagcggttg tcgcaccaga gatttctgcg acggtgggcg tggctgcggc ttttgcggtt
    25021 aaccccattg ttggcgcggc agtttttgcc gctagtaaag tgctggggcc gctgtggagc
    25081 aaagtctcca ttttgcgcta tcacatttcg ggtccgctgg acgatccgca aatcaacgaa
    25141 gtgttgcgcc aaccgcgtaa agaaaaagcg caatgatttg acgagggcgc gtaattgccc
    25201 caatctcata tgataatcgt tgccaaaggc caacgagcca gaacataacc gtaggccgga
    25261 tagggcgttc atgccgcatc cggcagccgt aaaaaatcct ctactgcagt aactaacgag
    25321 tagcaaaaac gatgagtctg aacctggtaa gtgaacaatt gctagcggcg aacagcctga
    25381 aacatcagga cttgttcgcg atcctcggtc aactggccga acgtcgcctt gattatggcg
    25441 atctctattt tcagtcgagc tatcacgaat cctgggtttt agaagaccgc attattaaag
    25501 atggttctta caacatcgat cagggcgttg gcgtgcgtgc aatcagcggt gaaaaaaccg
    25561 gatttgctta cgctgaccaa atcagcctgc tggcgctgga acagagtgcg caagcggcgc
    25621 gcaccatcgt ccgtgatagt ggtgatggca aagtacagac gctgggcgcg gtagagcata
    25681 gcccgttgta tacctcggta gatccgctgc aaagcatgag ccgtgaagag aagctggata
    25741 tcctgcgtcg cgtcgataag gttgctcgcg aagcggacaa gcgcgtacag gaagtgactg
    25801 ccagcctcag tggtgtctat gaattaattc tggttgcggc caccgacggc acgctagcgg
    25861 cggatgtccg tccgctggtg cgtctttccg tgagcgttct ggtcgaagaa gatggcaaac
    25921 gcgaacgcgg tgccagtggc ggcggcggtc gttttggtta tgagttcttc cttgccgatc
    25981 ttgacggcga agtccgtgcg gatgcatggg caaaagaagc agtgcgtatg gcgctggtca
    26041 atctttctgc cgttgctgca ccagcgggca ccatgccggt agtacttggc gcaggttggc
    26101 cgggcgtgct gttgcatgaa gcggtaggtc acggtctgga aggcgacttc aaccgccgcg
    26161 gcacttcagt atttagtggg caggtcgggg agctggtggc ttcagaactg tgtaccgtgg
    26221 ttgatgacgg cacgatggtc gatcgccgtg gttcggtggc gattgatgac gaaggtacgc
    26281 caggccagta caacgtgctg attgagaacg gcattctgaa aggctacatg caggataaac
    26341 tcaacgcgcg tttgatgggg atgacgccga ctggcaacgg tcgccgtgaa tcctacgccc
    26401 atctgcccat gccgcgtatg actaacacct atatgctgcc gggtaaatcg accccgcagg
    26461 aaattattga atccgttgag tacggtatct atgcaccgaa ctttggtggc ggtcaggtgg
    26521 atatcacctc cggcaaattc gttttctcca cttcagaagc atatctgatt gaaaacggta
    26581 aagtaacgaa gccggtgaaa ggcgcaacgt tgattggttc cggtatcgaa accatgcagc
    26641 agatttcgat ggttggcaac gacctgaaac tggataacgg cgtgggtgtc tgcggtaaag
    26701 aagggcaaag tttgccggtt ggcgtgggcc agccaacgct gaaagttgat aacctgactg
    26761 ttggcggtac tgcgtaataa tttcattatt ttcagaagga taattaatct ttctacgtgc
    26821 acgaacggtc ccctcgctcc tctggggtta gggtgagggg aacccgttgg cacaggtttg
    26881 tagaacgtaa cagtacaata tgaattactt ctctttcccg cgcccgtgca tctcctgaaa
    26941 caatttaccg acctcaacaa aataatccgt cagcgagttg atcacgacct gtaccttcag
    27001 cggcagctta tctttttcgg tatataacgc ataaaccggg cgtggatctg actgataacg
    27061 cggcagcagg atctccagct caccgcgatt gatctcgttg atcacccaca tcagcggcac
    27121 gtaggcgatc ccggcacccg ccgtcagcca gcgcaccagc gtcatcggat cattagtcac
    27181 aaatcttcct tgtgggatca ggcgagtcga gatcccttcc ggtgcgatca gttcaaattc
    27241 attgtcgggc cgcacgctgt attcaagcca tgaatgacta ctcaaatcgg cgggtttttc
    27301 cggtatgccg tattgtgtga gatagctttt cgcggcgcac accaccatcg gcatcgcgcc
    27361 cagacggcgg gaaaacaggc tggaattccg gtaaccagat tgacgctcaa acctgggtat
    27421 tctttcagca tttttgctgt cagcccggcg agaacatttt gtgccatagt tgaagaacag
    27481 ccaatgcgca gcgtcccgat gggggtgtta ttgaaggcat acagttgctc atgaacatcc
    27541 tgcacttcat gaagcatccg acggcagccc tggtagtaaa ttctgccggc ttcggtcagg
    27601 ccaatgctgc gtgtgctacg gtttaacagc tttacctgca actcatcttc cagttttgac
    27661 accgtctgac tgatggacga aacgctcatc tgtagctgtc tggcggcggc ggtaaaagag
    27721 ccaaattcaa ctactttggc aaacaccgac atgcgtttta gtcgttccat tattcactct
    27781 gacttaaaag tgatttagat cacataatat agataacagc ataacagtta cgctaatata
    27841 ttaaatatca atctacagca atgttgctct cgcccggctt tccatgccct tctctgcggc
    27901 gacagatgct gaaaataata acgcctgctc tctctttaca accaaggtca acatgagtct
    27961 gtttcccgtt atcgtggtgt ttggactgtc cttcccaccg atattttttg aattgctttt
    28021 atcactggcg attttctggc tggtgcgccg ggtacttgtg ccaacaggta tctacgactt
    28081 tgtctggcat ccggcgttgt tcaacaccgc gctctattgc tgcttgtttt atttgatatc
    28141 gcgactgttc gtttgaggtt gaagtgaaaa cactaataag aaaattctcc cgtacggcca
    28201 tcacggtcgt attagtcatt ctagccttca tcgcaatttt taatgcctgg gtctattaca
    28261 ccgaatcccc ctggacgcgt gacgcgcgct ttagcgctga cgtcgttgcg atcgcgccgg
    28321 acgtttctgg actcattacc caggtgaatg ttcatgataa ccagctggtg aaaaaaggac
    28381 agatactgtt caccatcgac cagccgcgct atcaaaaggc gcttgaggaa gcgcaagccg
    28441 atgttgctta ttatcaggta ctggcacagg agaaacgcca ggaggccgga cgtcgtaacc
    28501 gtctcggtgt gcaggcgatg tctcgcgaag agatcgacca ggccaacaac gtactacaaa
    28561 cggttctgca tcagttagcg aaagcgcagg cgacccgcga tctggcaaaa ctggatcttg
    28621 aacgcacggt gatccgcgcg ccagcagatg gctgggtgac caacctcaac gtctataccg
    28681 gtgagtttat tactcgagga tcaacggcgg ttgcgctggt gaaacagaac tccttctatg
    28741 tactggccta tatggaagaa actaagctgg aaggggggcg tccggggtat cgtgcagaga
    28801 tcacgccgct tggcagtaac aaagtgctga aagggactgt tgatagtgtt gccgcagggg
    28861 tcaccaacgc cagcagcacg cgtgacgaca aagggatggc gactatagac tctaatcttg
    28921 aatgggtgcg tcttgcgcaa cgtgttccgg ttcgtattcg tctcgacaac cagcaagaga
    28981 acatctggcc tgcgggcacc actgctacag tggtggtcac tggcaaacaa gatcgcgacg
    29041 aaagccagga ttcgttcttc cgtaaaatgg cccatcgcct gcgtgagttt ggttaatcac
    29101 gatgggtatt ttctccattg ctaaccaaca tattcgcttt gcggtaaaac tggcgaccgc
    29161 cattgtactg gcgctgtttg ttggctttca cttccagctg gaaacgccac gctgggcggt
    29221 actgacagcg gcgattgttg ccgccggtcc ggcctttgct gcgggaggtg aaccgtattc
    29281 tggcgctatt cgctatcgtg gctttttgcg catcatcggc acatttattg gctgtattgc
    29341 cggactggtg atcatcattg cgatgatccg cgcaccatta ttgatgattc tggtgtgctg
    29401 tatctgggcc ggtttttgta cctggatatc ctcgctggta cgaatagaaa actcgtacgc
    29461 gtgggggctg gccggttata ccgcgctgat cattgtgatc accattcagc cggaaccatt
    29521 gcttacgccg cagtttgccg tcgaacgttg tagcgagatc gttatcggta ttgtgtgtgc
    29581 gattatggcg gatttgctct tttctccgcg atcgatcaaa caagaagtag atcgagagct
    29641 ggaaagtttg ctggtcgcgc aatatcaatt aatgcaactc tgtatcaagc atggcgatgg
    29701 tgaagttgtc gataaagcct ggggcgacct ggtgcgacgc accacggcac tacaaggcat
    29761 gcgcagcaac ctgaatatgg aatcttcccg ctgggcgcgg gccaatcgac gtttaaaagc
    29821 gatcaatacg ctatcgctga cgctgattac ccaatcctgc gaaacttatc ttattcagaa
    29881 tacgcgcccg gaattgatca ctgatacttt ccgcgaattt tttgacacgc cggtagaaac
    29941 cgcgcaggac gtccacaagc agctcaaacg cctgcggaga gttatcgcct ggaccgggga
    30001 acgggaaacg cctgtcacca tttatagctg ggtcgcggcg gcaacgcgtt atcagcttct
    30061 caagcgcggc gttatcagta acacaaaaat caacgccacc gaagaagaga tcctgcaagg
    30121 cgaaccggaa gtaaaagtag agtcagccga acgtcatcat gcaatggtta acttctggcg
    30181 aaccacactt tcctgcattc tgggcacgct tttctggctg tggacgggct ggacttccgg
    30241 cagtggtgca atggtgatga ttgcggtcgt gacgtcactg gcaatgcgtt tgccgaatcc
    30301 acgcatggtg gcgatcgact ttatctatgg gacgctggcc gcgctgccgt tagggctgct
    30361 ctactttttg gtgattatcc ctaataccca acagagcatg ttgctgctgt gtattagcct
    30421 ggcagtgctg ggattcttcc tcggtataga agtacagaaa cggcgactgg gctcgatggg
    30481 ggcactggcc agcaccataa atattatcgt gctggataac ccgatgactt tccatttcag
    30541 tcagtttctc gacagcgcat tagggcaaat cgtcggctgt gtgctcgcgt tcaccgttat
    30601 tttgctggtg cgggataaat cgcgcgacag gactggacgt gtactgctta atcagtttgt
    30661 ttttgccgct gtttccgcga tgactaccaa tgtggcacgt cgtaaagaga accacctccc
    30721 ggcactttat cagcagctgt ttttgctgat gaataagttc ccaggggatt tgccgaaatt
    30781 tcgcctggcg ctgacgatga ttatcgcgca ccagcgcctg cgtgatgcgc cgatcccggt
    30841 taacgaggat ttatcggcgt ttcaccgaca aatgcgccgc acagcagacc atgtgatatc
    30901 tgcccgtagc gatgataaac gtcgtcggta ctttggccag ttgctggaag aactggaaat
    30961 ctaccaggaa aagctacgca tctggcaagc gccaccgcag gtgacggaac cggttcatcg
    31021 gctggcgggg atgctccata agtatcaaca tgcgttgacc gatagttaag tcaaaaccga
    31081 cgccaaaagc gtcggttttt tcatggctat acttagcgat gcacggcaga actcgccgcg
    31141 aaacgtgacg gtggcaacag atgaatattt atacctttga ttttgatgag attgagagtc
    31201 aggaggattt ttatcgtgac tttagccaaa cctttggtct ggctaaagat aaggtacgcg
    31261 atctcgactc actatgggat gtgttaatga acgatgtcct gccgctacca cttgagattg
    31321 aatttgttca tctgggagag aaaacgcgtc gccgttttgg cgcgttaata ttgctgtttg
    31381 atgaggcaga ggaagagctg gaagggcatt tgcgttttaa tgttcgtcat tagcgcaaaa
    31441 aaaagccccc gaaccggggg caatatcgtc ggacaagacg atgagggttt atttgtacag
    31501 ttcagccgta gcgtgccagg tgtcaccgct acgagcttca gtaatctggt aggccgttgc
    31561 gcctttctct tccgcttttt tgttcagcat ttcacgcata tccattggcg aagacgccac
    31621 accacttacg gatacggtcc cgattgcttc acgattttgt gcttgtgcag tatcaatgga
    31681 gtcggcagcg aatgcaccga aagagagaac agaaagtacg cttaatgcag ctacagtggt
    31741 tttgattttc atgattttta cctcgacata atcttttagc tgggcctttg tttcgtgatc
    31801 catatcacaa aatcaagcat acactaatta ttgaactaat taataccaca ctaactgttt
    31861 cagttgatac taagtgttaa aaatgtgaat tttaataaca aaaaagaatt aaaaacacgg
    31921 ttatttattg ttaattctag tattttcaat tagataatga attttacata atgtgctttt
    31981 tttaccgtat gtattcattg tgtgaatgat atgtcgcggt aaaacgcact attcgttaaa
    32041 ttaatcgcag gggaaagcag ggggttgaga ggaataagca acattttccc cgccgccaga
    32101 agcgacgggg cagagattaa agctcctggt cgaacagctc taaaatcgct tcgtacaggt
    32161 ctttgacggt gaaaccgtta gcaggggtgg taaagatggt gtcatcgcca gcgatggtgc
    32221 ccagaatacc ttctgctttg cccagtgagt ccagcaggcg agcaattaac tgcgccgcgc
    32281 cagggctggt atgaatcacg acaactgcat cgttgtagtc gatatccagc accagattct
    32341 tcaatggact ggaggtggtt ggtacaccca gttcagctgg caggcagtaa accatttcca
    32401 ttttggcatt gcgtgtacgt acagcaccaa acttggtcaa catccgcgag actttagact
    32461 gattaatatt gtcaaagcct tgctcctgca acgcggcgac gatttcgccc tgggagctaa
    32521 atttctcttc tttaagtaat gctttaaatg ctttaactag ttcttcttgc ttagccgagc
    32581 ttcgcataag tcacccgata tggtggttga tacaacatta ttgtgcatac agatgaattt
    32641 ttatgcaaac agtcagccct gaagaaggct gaaataatgt tatgaaagag cgggatttta
    32701 tcaaatttcg ttattgagaa acatgcctgc gtcacggcat gcaaattctg tttaaaagta
    32761 aattaattgt tatcaaattg atgttgtttt ggctgaacgg tagggtatat tgtcaccacc
    32821 tgttggaatg ttgcgctaat gcataagcga ctgttaatta cgtaagttag gttcctgatt
    32881 acggcaatta aatgcataaa cgctaaactt gcgtgactac acattcttga gatgtggtca
    32941 ttgtaaacgg caattttgtg gattaaggtc gcggcagcgg agcaacatat cttagtttat
    33001 caatataata aggagtttag gatgaaagtc gcagtcctcg gcgctgctgg cggtattggc
    33061 caggcgcttg cactactgtt aaaaacccaa ctgccttcag gttcagaact ctctctgtat
    33121 gatatcgctc cagtgactcc cggtgtggct gtcgatctga gccatatccc tactgctgtg
    33181 aaaatcaaag gtttttctgg tgaagatgcg actccggcgc tggaaggcgc agatgtcgtt
    33241 cttatctctg caggtgtagc gcgtaaaccg ggtatggatc gttccgacct gtttaacgtt
    33301 aacgccggca tcgtgaaaaa cctggtacag caagttgcga aaacctgccc gaaagcgtgc
    33361 attggtatta tcactaaccc ggttaacacc acagttgcga ttgctgctga agtgctgaaa
    33421 aaagccggtg tttatgacaa aaacaaactg ttcggcgtta ccacgctgga catcattcgt
    33481 tccaacactt ttgttgcgga actgaaaggc aaacagccag gcgaagttga agtgccggtt
    33541 attggcggtc actctggtgt taccattctg ccgctgctgt cacaggttcc tggcgttagt
    33601 tttaccgagc aggaagtggc tgatctgacc aaacgcatcc agaacgcggg tactgaagtg
    33661 gttgaagcga aagccggtgg cgggtctgca accctgtcta tgggccaggc agctgcacgt
    33721 tttggtctgt ctctggttcg tgcactgcag ggcgaacaag gcgttgtcga atgtgcctac
    33781 gttgaaggcg atggtcagta cgcccgtttc ttctctcaac cgctgctgct gggtaaaaac
    33841 ggcgtggaag agcgtaaatc tatcggtacc ctgagcgcat ttgaacagaa cgcgctggaa
    33901 ggtatgctgg atacactcaa gaaagatatc gccttgggcg aagagttcgt taataagtaa
    33961 ttgattagcg aataataaaa aaccggagca cagac
//
LOCUS       DAJVQG010000028        33769 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJVQG010000028 DAJVQG010000000
VERSION     DAJVQG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33769
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.028"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            3..505
                     /locus_tag="OQM83_001223"
                     /pseudo
     CDS             3..505
                     /locus_tag="OQM83_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(572..2029)
                     /gene="nuoN"
                     /locus_tag="OQM83_001224"
     CDS             complement(572..2029)
                     /gene="nuoN"
                     /locus_tag="OQM83_001224"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCS1519526.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2036..3565)
                     /gene="nuoM"
                     /locus_tag="OQM83_001225"
     CDS             complement(2036..3565)
                     /gene="nuoM"
                     /locus_tag="OQM83_001225"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCS1519527.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3729..5570)
                     /gene="nuoL"
                     /locus_tag="OQM83_001226"
     CDS             complement(3729..5570)
                     /gene="nuoL"
                     /locus_tag="OQM83_001226"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCS1519528.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5567..5869)
                     /gene="nuoK"
                     /locus_tag="OQM83_001227"
     CDS             complement(5567..5869)
                     /gene="nuoK"
                     /locus_tag="OQM83_001227"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCS1519529.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5866..6420)
                     /gene="nuoJ"
                     /locus_tag="OQM83_001228"
     CDS             complement(5866..6420)
                     /gene="nuoJ"
                     /locus_tag="OQM83_001228"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCS1519530.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6432..6974)
                     /gene="nuoI"
                     /locus_tag="OQM83_001229"
     CDS             complement(6432..6974)
                     /gene="nuoI"
                     /locus_tag="OQM83_001229"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCS1519531.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(6989..7966)
                     /gene="nuoH"
                     /locus_tag="OQM83_001230"
     CDS             complement(6989..7966)
                     /gene="nuoH"
                     /locus_tag="OQM83_001230"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCS1519532.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(7963..10689)
                     /gene="nuoG"
                     /locus_tag="OQM83_001231"
     CDS             complement(7963..10689)
                     /gene="nuoG"
                     /locus_tag="OQM83_001231"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCS1519533.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10742..12079)
                     /gene="nuoF"
                     /locus_tag="OQM83_001232"
     CDS             complement(10742..12079)
                     /gene="nuoF"
                     /locus_tag="OQM83_001232"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCS1519534.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12076..12576)
                     /gene="nuoE"
                     /locus_tag="OQM83_001233"
     CDS             complement(12076..12576)
                     /gene="nuoE"
                     /locus_tag="OQM83_001233"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCS1519535.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12579..14381)
                     /gene="nuoC"
                     /locus_tag="OQM83_001234"
     CDS             complement(12579..14381)
                     /gene="nuoC"
                     /locus_tag="OQM83_001234"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCS1519536.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14475..15137)
                     /gene="nuoB"
                     /locus_tag="OQM83_001235"
     CDS             complement(14475..15137)
                     /gene="nuoB"
                     /locus_tag="OQM83_001235"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCS1519537.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15153..15596)
                     /gene="nuoA"
                     /locus_tag="OQM83_001236"
     CDS             complement(15153..15596)
                     /gene="nuoA"
                     /locus_tag="OQM83_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCS1519538.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16225..17163)
                     /gene="lrhA"
                     /locus_tag="OQM83_001237"
     CDS             complement(16225..17163)
                     /gene="lrhA"
                     /locus_tag="OQM83_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCS1519539.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPYLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18078..19295
                     /gene="alaA"
                     /locus_tag="OQM83_001238"
     CDS             18078..19295
                     /gene="alaA"
                     /locus_tag="OQM83_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCS1519540.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19379..19978
                     /gene="yfbR"
                     /locus_tag="OQM83_001239"
     CDS             19379..19978
                     /gene="yfbR"
                     /locus_tag="OQM83_001239"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCS1519541.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVNMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20037..21869)
                     /locus_tag="OQM83_001240"
     CDS             complement(20037..21869)
                     /locus_tag="OQM83_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCS1519542.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(21956..22604)
                     /gene="hxpA"
                     /locus_tag="OQM83_001241"
                     /pseudo
     CDS             complement(21956..22604)
                     /gene="hxpA"
                     /locus_tag="OQM83_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(22615..23109)
                     /locus_tag="OQM83_001242"
     CDS             complement(22615..23109)
                     /locus_tag="OQM83_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCS1519543.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23192..23647)
                     /locus_tag="OQM83_001243"
     CDS             complement(23192..23647)
                     /locus_tag="OQM83_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCS1519544.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            23985..25187
                     /gene="ackA"
                     /locus_tag="OQM83_001244"
     CDS             23985..25187
                     /gene="ackA"
                     /locus_tag="OQM83_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCS1519545.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25262..27406
                     /gene="pta"
                     /locus_tag="OQM83_001245"
     CDS             25262..27406
                     /gene="pta"
                     /locus_tag="OQM83_001245"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCS1519546.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            27596..29116
                     /gene="yfcC"
                     /locus_tag="OQM83_001246"
     CDS             27596..29116
                     /gene="yfcC"
                     /locus_tag="OQM83_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCS1519547.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGTRMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29149..29691)
                     /gene="yfcD"
                     /locus_tag="OQM83_001247"
     CDS             complement(29149..29691)
                     /gene="yfcD"
                     /locus_tag="OQM83_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCS1519548.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(29749..30300)
                     /gene="yfcE"
                     /locus_tag="OQM83_001248"
     CDS             complement(29749..30300)
                     /gene="yfcE"
                     /locus_tag="OQM83_001248"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCS1519549.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30356..31000)
                     /gene="yfcF"
                     /locus_tag="OQM83_001249"
     CDS             complement(30356..31000)
                     /gene="yfcF"
                     /locus_tag="OQM83_001249"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCS1519550.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31136..31783
                     /gene="yfcG"
                     /locus_tag="OQM83_001250"
     CDS             31136..31783
                     /gene="yfcG"
                     /locus_tag="OQM83_001250"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCS1519551.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLTEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            31840..32202
                     /gene="folX"
                     /locus_tag="OQM83_001251"
     CDS             31840..32202
                     /gene="folX"
                     /locus_tag="OQM83_001251"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCS1519552.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32223..33116
                     /locus_tag="OQM83_001252"
     CDS             32223..33116
                     /locus_tag="OQM83_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCS1519553.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVFISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33164..>33769)
                     /gene="rpnB"
                     /locus_tag="OQM83_001253"
     CDS             complement(33164..>33769)
                     /gene="rpnB"
                     /locus_tag="OQM83_001253"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCS1519554.1"
                     /translation="MRYSIAAMQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDE
                     FAEPAIARKIYSSAFPLVDITVVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVS
                     LLVTGNTNDRQLKALFNYVLQTGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGA
                     MQGKHEEALRIAQEMLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         8410 a         9224 c         8520 g         7615 t
ORIGIN      
        1 aaatgggaat gattggttat tttgcagaaa ttgactcaga aaagattaac caactgcttg
       61 agtctactga aaaacctttg atggataata tacatgacac gctttcagga ttacgccgac
      121 tggatattga taaacgctgg gattttttac actttgggct gacaggcacc cctgcttttg
      181 atcccgcgaa gaatgatccg ctgagtcgtg ccgtgcttgg tgaacacagc ctcgaagatg
      241 gcatagatgg cttcctggga ttgacctgga atcaggagtt agccgcgaca atcgaccgac
      301 tggaatcact ggatcgaagt gagctgcgta aacagttttc cattaagcga ctcaatgaaa
      361 tggagattta tccgggagtg accttttcag aagagttaga ggggcaactc tttgcttcga
      421 tcatgctgat atggaaaagc tgattagcgc gtatcgacgt atgctgcgac aagggaatca
      481 tgcgctgacg gttattgttg ggtgatggtg tcagggcacc aaaaaaaacc gccgaacatg
      541 tcggcggctt tctgacttac aaactgatag cttacatcag cggcattgct aaccgcacaa
      601 tgctaatcag cggttgtggc catacaccca gcaccagtac caacagtgca gagatcagca
      661 ccacgatacc gcctgcgctg tactgccagt ttgacggtgc atcgcgaccc ggttgttccg
      721 gggcgtgcag gtacaggctc accgccacgc gcaggtagta gtaaaggccg cttgccgaac
      781 cgacaaccac ggcgcccacc agccaccaca agtgtgcctg gacaccgacc gccagcacgt
      841 agaacttacc aataaagccc agcgtcatcg gaataccggc cagagacagc atcatcaccg
      901 tcatcaccgc cgcgaggatc gggcgatgcc agaacagtcc gcggtaagag tacagggaat
      961 cagcatccgg gccacgatac gggctggaca tcaggctgac cacgccgaac gcgccgaggc
     1021 tgctaaacag ataaccggcc aggtaaaccc ctaccgcttc catcgacatc tcgccagttt
     1081 gcagcgcaat cagcgccacc agcagatagc cgaggtgaga gatagatgag taaccgagca
     1141 gacgtttgat attggtctgg ctcagcgcca tcaggttacc gaagatgatg gaggcaaagg
     1201 cgataatcgc cagcaccacg cgaatcgctt cgctgtcacc catcggtgcg tagaggaaca
     1261 gacgcatcac cacaccgaag atagcgattt tgctcgccgt cgccaagaaa gtggaaaccg
     1321 gcgcaggcgc gccctggtat acgtctggcg tccacaggtg gaacggcacc agagagagtt
     1381 tgaagccgag gccaacaatc atcaggccga aacctgccag caacagcggc tcgttgagca
     1441 taccgtcgcc aaggttttta cccaacgcga caaacgacag gtcgccagac tgcgcataca
     1501 ccagcgccat accaaacagc aggaaagaag acgctgcggc agaaaggatg gtgtatttga
     1561 tactggcttc cagtgaacgt ttctggcgga aagcgtaacc gaccaggcca aacagcggca
     1621 aagagatcag ttcgataccg aggaacagag acgccagatg gttggcattc gccagcagga
     1681 tcccgcccag cgcggcaatt aacaccaaca ggtagaactc gtccttgttg tcgttatagc
     1741 cttcaagcca cgggtaggcg aaagtacagg tggcgaggct cgccaacaat accagcccgg
     1801 tgtaaagcat ggcgaaacca tcaacgcgca tcagcggcgt aacgtccata gcgcctgcct
     1861 ggccgacaaa ccagagcgaa accagcgccg cgttaagccc aataaccgag agcgtagcgt
     1921 tgaggaaatg attgcgtcgc cacgcaatgg agagcatcac aaccaccacc gtcaagccga
     1981 cgatcagcaa cggtagcagt gcgatcaggt tttgtggagt tattgtcatg gcgatttacg
     2041 gccttgtagt agtaacggaa ttaacaaacc actgctggat attgccaatc gcggagtgcg
     2101 aggtatccag aatcggctgc ggatagaagc cctgcagtac cagcagcacc accagcaaca
     2161 ggatcataaa cagctcacgc agcgacatcc ctggcagttc ctggctggca atctcgcttt
     2221 tcgctttacc gaagtaagcg cgatgcaaca tcgccagcga ataaacagat gcaaagacca
     2281 gcccaaaggt agagataacg gtaatcaccg ggacaacctg gaagctgccg aacagaatca
     2341 taaattcgcc gacgaagtta ccggtgccag gcatcccaag cgttgccacc gcaaagaaca
     2401 gcgacagtgc tggcagccat ttcatcttgc tccacagacc gcccatcatg cgcatgtcgc
     2461 gggtatggat acgttcataa agctgaccac aaagaataaa cagacccgcc gccgacaagc
     2521 cgtgcgcaat catctggatt accgcgccct ggtaggccaa ctggctgccg gtgtagatag
     2581 caatcagcac gaagcccatg tgggaaaccg aggtgtaggc gatcagacgt ttgatatcgg
     2641 tctgggcgaa ggccatccac gcaccgtaga agatgccgat aacacccagc cacatagcaa
     2701 ttggcgcgaa ctctgccgac gcgttcggga acagcggcag ggagaaacgc agcaaaccgt
     2761 aagcggcagt tttcagcaag atccccgcga ggtcaacgga accggcggtc ggagcctggg
     2821 agtgcgcatc cggcagccag ccatgcagcg gaaccaccgg cattttgact gcgaaggcga
     2881 tgaagaagcc cagcattaac aggtattccg caccactgga cattggcgta ttcagcagct
     2941 cttcatagtt gaaggtccag acgccggtcg cattgtagtg aacaaaaacc agcgccagga
     3001 tggcaatcaa catcaccaga ccactcgcct gggtgtaaat gaagaacttg gttgccgccg
     3061 tgatacgcgt tttaccgtca gaggctttat gcccccacag tgcgatcagg aagtacatcg
     3121 gcaccagcat catttcccag aagaagaaga acaggaacat gtcgatggca aggaacacgc
     3181 cgataacgcc gcccaggatc cacatcaggt tgaggtggaa gaagccctga tatttttcga
     3241 tctctttcca cgaacatagt accgccagca caccgagcag accggtcagc acgaccatca
     3301 gcagcgacag cccgtcaatg gcgagatgaa tagagatacc caaacgcggg atccacggca
     3361 tgtcgaattc agactgccac tgcggaattc cggcggattg cgtcagtgaa taaccgccct
     3421 gcaaccacag ttgcagcgac agcgccagcg tcaatcccat ggtgatcagc gcgatccagc
     3481 gtggcacctt gacgccaaag cgttcggtct gccagcacag gaagccgcca ataaagggaa
     3541 ttaatattag ccagggtagt aacatggcga tctttattcc ttgtaaaagt cccgtcagga
     3601 ccggattttc aacgaattct cacgacagaa aattctcttc ggactggggg tagtgtcgga
     3661 tgcaccgcga aggcggctta tccgacctac gttcttatat gcccaggggc aatcccacaa
     3721 tccttaactc aacgcagtac catcaacagt gccagcacca cgaccgcacc gatgctcatg
     3781 gatgccacat accagcgcag atagccgttc tcacttaaca gcagaccttt acctgcaaag
     3841 cgggaaagga cagccggaat gttcatcatt gagttcagcg gatcgcgttt cagcaaccag
     3901 gcaataccca ggaacggcct gacgaacact ttgtcgtaca gccagtcaaa gccccaggcg
     3961 ttgtaccacc aggtactcag caggcggccc ggcgcactgt tggcgatgga ggtcaccaga
     4021 gtacgtttac ccagccacag ccaagctgcc agcagaatgc cgacaatcgc gaccacgcca
     4081 gaggtaattt ccagggtcaa catgctgccg tgcgccagtt ccgtcgtttg cggaagcacg
     4141 ccctgcagcg gcggtacaat cagtgcgcca acgaaggtgg aaaggatcag cagcacaatc
     4201 agcggcaggc tgtgagttac ccctttcacg gcgtgagcgt gaatttgttc ttttccgtgg
     4261 aagacgatga aaatcatacg gaaggtgtag agcgaggtca taaacgcacc gaccagacct
     4321 gccaccatca gattgatatg accattcgcc atcgcacccg cgaggatctc atccttactg
     4381 aagaagcccg cagtgaccag cggcagtgcc gacagtgctg cgccgcccac caggaagcag
     4441 agataaacca gcggaataga tttacgcaga ccgcccatct tgaagatgtt ctgttcgtga
     4501 tggcaggcca gaatgacgga accggatgcc aggaacagca gcgctttaaa gaacgcgtga
     4561 gtcatcaagt ggaaaatcgc cgcatcccat gcctgcacgc caagcgcgag gaacatgtag
     4621 ccaatctggc tcatggtaga gtaagcgaga acacgtttga tgtcggtctg taccagcgcg
     4681 gcaaaaccgg ccagcagcag cgtaaccgcc ccgacaatgc ccaccagatg cagaatttcc
     4741 ggcgtcatca ggaacaggcc gtgggtacgg gcgatcaggt agacacccgc ggttaccatg
     4801 gttgcggcgt ggatcagcgc ggagacaggc gtcgggcccg ccatcgcgtc ggcaagccat
     4861 gtctgcaacg gcaactgcgc agatttaccg accgcaccgc ccagcagcat cagcgtcgcc
     4921 cacatcagca tgttattgcc gtcagcaaag tgcgctggtg ccagttccac catttcgcgg
     4981 aagttcaggg tgcccagttc gttgtaaaga atgaacagtg cgaaagcgag gaaaacgtca
     5041 cccacacggg tcacgacgaa cgctttcatt gccgctgcgc cattcttcgg atcggtgtaa
     5101 tagaaaccga tcagcagata ggagcacagg cccacgcctt cccagccgag gtacatcagc
     5161 agcaggttgt cggcaagcac cagaactacc atgctggcga tgaacaggtt ggtgtaggcg
     5221 aagaagcgag agtagccctc ttcaccgcgc atgtaccagg aggcgtacat gtgaataagg
     5281 aaacccacac cagtgaccac cgagagcatg gtcagcgaca ggccgtccag caccaggtta
     5341 aaaccgatgt taaagtcgcc taccgacatc cacgtccaca gcggctggct gtatgcctgc
     5401 tcgccgttag cgaagaaatc aacgccgata aaggcggtta ccagcgccgc caggcccaca
     5461 gagccaacgc cgacgatcgc cgaaacgttt tcagaccagc gcccacggga gaacgccagc
     5521 aggacgaagc caatcaatgg caaaataatg gttaaggcaa gcatgttcat ccgcgcatct
     5581 cacttactga atcgatgttc aggttctggc gacgacggtg aagttgcagc agcagcgcaa
     5641 ggccgatact cgcttctgcc gccgcgaggc tgatggcgag aatgtacatc acctgaccgt
     5701 cggtctgacc ccagtagctt ccggcgacaa caaaggccag cgcggaggcg ttaatcatga
     5761 tttccagacc aatcagcata aacagcagat tgcgacggat aaccagaccg gttaagccaa
     5821 gaatgaataa gattgccgcg aggatcagtc catgttgtaa ggggatcatg cgtgctcctc
     5881 cgtttttctt ttcgcgctgt cgtctttacg attgctcagc acttcacccg cacgctcttc
     5941 acgaccgacg tggaaggcca caaccagacc tgcgagcagc agcatagaag ccagttccac
     6001 cgccagtacg taaggcccga acagcgtaat acccactgct ttagcactga ttggcgtacc
     6061 gtcgataccc tgatcgttaa caccgaggat ggcgtaaaca atcaccacca gcatgatggc
     6121 cgacaaaatt gccggaccaa tccacacctg cggtttcagc cactggcgtt cctgttcgat
     6181 ttctgaaccg cccaggttga gcatcatcac cacgaacacg aacagcacca taatggcacc
     6241 cgcgtagacg ataatttcca gcgcaccggc gaagtaagcg cccagtgaga agaacacccc
     6301 ggaaatcgcc agcagcgaaa taatcaggta cagcagtgcg tgtaccggat tggtatgggt
     6361 gatcactcgc aaggtcgcaa gtatggctat caggccacag atataaaaag cgaactccat
     6421 tgcccctctc cttacggtaa caggctcttg acgtcgatag gcttggcttc gttctctgct
     6481 tcgcccttat ctttgccgtc gattgccata cctgccatcc ggtagaagtt atattccggg
     6541 tatttgcccg gaccggagat cagcagatcc tctttttcgt aaaccagatc ctggcgcttg
     6601 tattccccca tttcgaaatc cggggttaac tgaatcgccg tggtcggaca ggcttcttcg
     6661 cacagaccac agaaaatgca gcgtgagaag ttgatgcgga aaaattccgg ataccagcga
     6721 ccgtctttgg tttctgcttt ttgcagcgag atacagccga ccgggcaggc taccgcgcag
     6781 aggttacagg ctacgcaacg ctcttcgccg tccgggtcgc gggtcagaac gatacgacca
     6841 cgataacggg gcggcagata gatcggctct tccgggtaca ttcgcgtttc gcgtttggcg
     6901 aacgcgtgca ggccgatcat ccagatacta cgaacctggg tgccgaaacc tactaacaat
     6961 tcttttaagg tcatggtctt attgcccctt attgcgcctg ccagagaatg acagccgccg
     7021 ttaccagcaa gttgatcagc gtcagcggca ggcagatttt ccagccgaag gacattacct
     7081 ggtcataacg cggacgcggt aacgacgcac gaatcaaaat gaacatcatc ataaagaacg
     7141 cggttttcag cgcgaaccag atgaatggcg gtaacaacgg gccttgccag ccaccgaaga
     7201 acagcgtcac catcaatgca gagatggtca caatcccgat gtactcaccc acgaagaaca
     7261 gaccgaactt cataccggaa tattcaatgt ggtaaccatc cgccagttcc tgctcggctt
     7321 ccggctggtc aaacgggtga cggtgacata ccgccacgcc cgcgatggca aaggtaataa
     7381 aaccaaagaa ttgcgggata acgttccaca catgcgcctg gctgttgacg atgtcggtca
     7441 tgttgaatga accggcctgc gccaccacgc ccatcaggga aagcccgagg aacacttcgt
     7501 agctcagggt ctgcgcagaa gcacgcatcg cacccagcaa cgagtatttg ttgttacttg
     7561 accagcccgc aaacagcacc gcgtaaaccg ccagacctgc catcatcagg aagaacaaaa
     7621 tcccgatgtt caggtcggca accacccaac ccggactgac tggcacaatc gcaaaggcca
     7681 gcagcagcga ggtaaaggca atcatcggtg ccagggtaaa gatgacgcga tccgagaatt
     7741 tcgggatcca gtcttcttta aagaacattt tgatcatgtc cgcaaccagc tggagcgaac
     7801 cgccccagcc aacacggtta ggtccgtaac ggttctggaa cagacccagc aggcgacgtt
     7861 cgccaaagct catgaatgcc ccgcaggtga caaccaccag caggatcacc accgctttga
     7921 ggatggtcag caggatctca atcagttccg gtgatatcca actcattgtt gtgcctcctt
     7981 gagatcctca agatgcgcgc cagccagcac cggagcaata ccggacatac ccatcggcaa
     8041 gcccacctgc cctgccgtca gcccttcagc gatttcaacc ggcagcgtga ccgtattgcc
     8101 atcgtaacta aaggagacgt gtgtacctgc gttcacacct aacttcgcgg catccgctgg
     8161 gttgagtttg atgtacggct gcggcatacg gctctggaag accggagcac gctgtgacaa
     8221 ttcatcgctg ccaaacaggt ggtaatacgg cgcgatacgc catttcccgt cctgcggctg
     8281 gaagcgtgcc ggtacgctgg tgaagtaatc cagaccattt tcgctggttt caaacagacg
     8341 cacgcccgga tcgccaaagc gcagtttgcc gcccacttcg tcctggaatt tgttccacgc
     8401 ctgcggggag ttccagcccg gcgcccaggc aaacggcact tgcgaacggt gcgcagtcgg
     8461 ctggttgtta ccttccatcg agaaggtgaa catggtgtca atatcctgcg gctgacgcgg
     8521 ctcatgaacg ctgatattgg cgcgcatggc ggtacgaccg ctgtaacggt gcggttcacg
     8581 ggccagtttc tgcccacgaa tacggaatgt cgcatccggc gcagcatctt tgatacctgc
     8641 cagttccggg attttcgcca caacagcgtc aatcacatgg tcgagctgcg tccagtccac
     8701 ttcacggctc agcagggtgc tgtgcagcga gtgtaaccag cgccagcttt ccagcatgac
     8761 agttttgctg tcgtaataag caggatcgta aacctggaag aaacgttggg cgcggccttc
     8821 gttgttgatc accgtaccgt cgctttcagc aaaactggca gcagaaagta ccagatgggc
     8881 gttttccata atcgctgtgc gttgatgatc aaccaccatc accagcggcg ctttagccag
     8941 cgcagcattc acgcgggtag cggaagcgtg acgatgcaga tcgttttcca gcaccaccac
     9001 cgcgtcggcg cgtccggttt ccagttcggt caacgcttct tcaagcgaac cgccacccat
     9061 aatgcccagc cccatgctgt tgacggaacg ggcaatcatg gtgataccaa cgtcagcgcc
     9121 gcgacctttc agggctttcg cgacgttagc cgcagcctga atcacctcta tgctaccggc
     9181 gttcgtcccg gagataatca acggtttctt cgcacctgcc agtgcctgca cgatgacgtc
     9241 gattttgctt tgcagctcag gttcgatacc gtcaaccgct ggcgcagagt tatccagcgc
     9301 atgggcgatg gcaaaaccta aacgcgcctg atcttcaacc ggtgcgcggt aagtccacgc
     9361 cgcgatatca tccagacggg tgtcatcaac gttggtaaca aacagcggat gcttcgcacg
     9421 ttgaccgatg ttgaggattg ccgcaatctg ccagtcagcc actttctgtg ctgccgccat
     9481 ttcgcgcgct ttacctttca cagcctgacg cactgccagc gcgacgcgcg cgccggtctg
     9541 ggtaacgtct tcgcccagca ccagtaccgc atcgtaagat tcgatttcgc gcagagccgg
     9601 agtataaatg ccgccttcac gcagcacttt cagcgccagt tgcagacgtt cctgctcacc
     9661 gtgagcgata ccggtgtaga agttttcttc gcccaccagt tcacgcagcg caaagttgct
     9721 ttccacgctg gcacgcggag aaccaatacc gatcactttc ttcgactgac gcagaatatc
     9781 tgccgcgccc tgcattgcct gttcggcgtt gagggtaatg aaatcatcgc cacgacgctg
     9841 tactggctga cgcggacgat ccttcaggtt gacgtaaccg taaccgaaac gaccacggtc
     9901 gcagaggaag tagtggttta ccgtaccgtt gtaacggttt tcgatacgac gcagttcgcc
     9961 gtaacgttca ccggggctga tgttacagcc gatggaacat tgctggcaga tgctcggcgc
    10021 aaactgcata tcccatttac ggttgtaacg ctcggagtgc gttttgtcgg taaatacgcc
    10081 ggtcgggcaa atttcgacca ggttaccaga aaattcgctt tccagcgtgc cgtcttccgg
    10141 gcgaccgaag tagacgttgt cgtgcgcacc gtaaacgccc agatctgtac cgtcagcgta
    10201 atctttgtag taacgcacac agcggtagca ggcgatgcag cggttcattt cgtgagagat
    10261 gaatggcccc aaatcctgat tacggtgggt acgtttggtg aaacggtagc gacggaagct
    10321 gtgtccggtc atcacagtca tatcctgaag atggcagtta ccgccttctt cacataccgg
    10381 acagtcgtgc gggtggttgg tcattaacca ctcgaccaca ctttcacgga actgtttcgc
    10441 ttcttcgtcg tcaatggaaa taaaggtgcc atcggaagcc ggtgtcatac aggacatcac
    10501 caggcgacca cgcgtgtctt ccgcgttttg gtattgcttc accgcacact ggcggcaagc
    10561 accgacgctt cccagcgccg gatgccagca aaagtaagga atatcaaggc ccagagacag
    10621 acaagcttcc agcaggttgt ccgctccgtt gacctcgtat tctttgccgt ctacatgaat
    10681 tgtagccatt agcatgcttc cagttttctc ggtcagggac cgagcgttaa tcgaaattcg
    10741 gttaccagcg ctctttcagc aggttcggct gaatcccatt aatcaaatgg gtattgctga
    10801 acggctgttt gattcccgcc tcaaattctt cgcggaaata tttgatggcg ctctgtaacg
    10861 gctccactgc accaggtgcg tgggcacaga aagttttacc cgggcctaag aatcgacaca
    10921 gttgctcaag tgtttcgata tcgcccggct ggccttcgcc acgctccagc gcacgcagaa
    10981 ttttcacgct ccacggcaga ccgtcgcggc acggcgtaca ccagccgcag gactcacggg
    11041 cgaaaaactc ttccaggtta cgcaccagcg acaccatgtt gatctcatgg tcaaccgcca
    11101 tcgccagcgc cgtacccaga cggctgcccg ctttaccgat actttcgaat tccatcggca
    11161 gatcaaggtg cgcttcggtc aggaagtcag tccccgcgcc gcctggctgc caagctttaa
    11221 atttcagacc atcacgcata ccaccggcgt aatcttcgag gatctcgcgt gcggtggtgc
    11281 cgaacggcag ttcccacagt cccggatttt tcacccgacc ggagaagccc atcagcttgg
    11341 tgccagcatc tttacttttc gagatgttct gataccactc cacgccgtta gcgaggatcg
    11401 ccggaacgtt acacagggtt tcgacgttgt tgacacaggt cggtttgccc catacgccgg
    11461 aggttgccgg gaaggggggt ttcgagcgtg ggttagcacg acgcccttcc agggagttga
    11521 ttaacgctgt ttcttccccg cagatgtagc gccctgcccc ggtatggacg aacagttcga
    11581 agtcgaaacc tgttcccata atgtttttgc caagcaagcc cgcttcagtg gcttcggcaa
    11641 tggcgcggcg cagatttact gccgcttcga tatattcgcc acgcaggaag atgtagccac
    11701 ggtaagcttt cagcgcaaac gcggagatga gcataccttc caccagcagg tgcggcagtt
    11761 gctccatcaa caggcggtct ttataggtgc ccggctccat ttcatcggcg ttacacagca
    11821 ggtaacggat gttcatggat tcgtctttcg gcatcaggct ccacttcagg ccagtcgaaa
    11881 agcccgctcc gccgcgccct ttcagaccag cgtcttttac ctgattaacg atttcgtccg
    11941 gagacagccc ggtcagcgcc ttacgcgccc cttcgtaacc gtttttgctg cggtattcgt
    12001 ccagccacac tggctgtttg tcatcgcgca gacgccaggt cagcggatgc gtttcgggag
    12061 tacggataat gtttttcatt tataccgctc cagcagttca gggatcgctt ccggggtcag
    12121 atgcgcgtga gtgtcctcat cgatcatcat gtttggccct ttatcacagt tccccaggca
    12181 gcaagttggc agcagcgtaa agcggccatc aaatgtcgtt tgccctggtt tgatgttcag
    12241 ctttttctcg agcgccgcct gaataccctg atacccgttg atatgacaga ccacgctgtc
    12301 gcaataacgg atcacatggc gaccaaccgg ctggcggaag atctgactgt agaacgttgc
    12361 cacaccttcg acgtcgcttg ccggaatacc cagcacatcg gcgatcgcgt ggatcgcacc
    12421 atccggcacc cagccacgct gcttctgaac gattttcagc gcttcaatgg acgccgcacg
    12481 cgggtcttcg tagtggtgca tctcgtgctc aatcgcttca cgctctgccg cactcagctc
    12541 aaaagcctcg gtttgtggtt gttgattctc gtgcataatt agcggtccac atctgacata
    12601 acaaaatcga tactgcccag ataaacaatc aggtcagaca ccaggctgcc gcggatcgcc
    12661 gccggaattt gctgcaaatg agcataactc ggcgtgcgga tacgagtgcg ataactcatg
    12721 gtgctaccgt cgctggtcag gtagtaactg ttgatcccct tggtcgcctc aatcatctgg
    12781 aaagattcat tggcaggcat caccggcccc cacgacactt gcaggaagtg ggtgatcagg
    12841 gtttcgatat gttgcagcgt gcgctctttc ggcggcggcg tggtcagcgg gtgatccgct
    12901 ttgaacgggc cttccggcat gttgttgagg cactgctcaa gaatgcgcag actctggcgc
    12961 agctcttcca ctttcagcat tacgcgggtg tagcagtcag aaacaccacc tccaaccggg
    13021 atttcaaagt cgaagttttc atagccagaa taaggacgcg ccttacgcac gtcgaagtcg
    13081 atcccggtag cacgcaggcc cgcgccagtg gtgccccact ccagcgcctc tttcgcgcca
    13141 taggcggcaa cgccctggga acgacctttc agaatggtat tttgcagcgc cgctttctcg
    13201 taagacgcca gacgtttcgg catccagtcg aggaactcac gcagcaggcg atcccagccg
    13261 cgcggcaggt cgtgcgctac gccgccaata cggaaccacg ccgggtgcat acggaaaccc
    13321 gtgatcgctt ccaccagatc gtaaattttc tgacgatcgg taaaggcgaa gaacacgggc
    13381 gtcattgcgc cgacgtcctg aataaaggtc gagatgtaca gcaggtggct gttgatacgg
    13441 aacagttcgg agagcataac gcgaatgacg ttaacgcgat ccggcacggt gatcccggcc
    13501 agtttctcta ccgccagcac gtaaggcatt tcgttaacgc agccgccgag gtattcaata
    13561 cggtcggtat acggaatgta gctgtgccag gactggcgct cgcccatttt ctccgcacca
    13621 cggtggtggt agccgatgtc tggtacgcag tcgacaatct cttcgccgtc gagttgtaaa
    13681 acgatacgga aagccccgtg cgccgacggg tggttcggac cgaggttgag gaacatgaag
    13741 tcctcgtttt cggtgccgcg cttcatcccc cactcttccg gtttgaaggt cagggcttcc
    13801 atctccagat cctgtttggc tttggtcagc tcaaacggcg agaattcggt agcgcgcgcc
    13861 gggtaatctt tacgcagcgg gtgacctttc caggtttgcg gcatcatgat gcggcgcagg
    13921 ttcgggtgac cgtcgaaagt aatgccaaac agatcccagg tttcacgctc ataccagtta
    13981 gcgttcggga acagtttggt gaaggtcggt acgtgcaggt cgttttctgc cagcgccacc
    14041 ttcagcatga tgtcgcggtt acgatcgata gaaatcagat ggtagaaaac ggaaaaatcc
    14101 gcggcaggta acccttcgcg gtgtgtgcgc agacgttcgt ccatgccgtg taagtcaaac
    14161 agcatgacgt aaggtttcgg cagtttcttt aagaaatcgc caacttccag taattgttca
    14221 cgcttgatcc acacaacggg aaccccggtg cgagtcgcct gaacagtaaa ggcatccggc
    14281 ccaaaacggt tgcgcagttc gccaatcacc ggatcatcaa gatgatcacg ggtctgccag
    14341 gcgggttctt gcgcggttaa gtcggtcata ttgttcacca ttgcaaatgg tccgtggtga
    14401 ctgtcggcgc agtaagcttc gcgaaataga tttaggaata agcgaagaaa aatccctttg
    14461 ccgacaggcg caaattaaat ctcgtcaggt gtacgcaggt tggttacggc aatgcgttca
    14521 ccgcgcttgc gttcgcgctc tgattgcata ttggcgcgat aaacgccctg atcgccaacc
    14581 acccaggaga gcggacgacg ttctttgccg atagattcct gcaacagcat cagtgcctgc
    14641 atgtacgctt caggacgcgg cgggcagccc gggatataca catcaaccgg gatgaattta
    14701 tcgacgccct gcacaacgga ataaatatcg tacataccac cagagttggc acaggcaccc
    14761 attgagataa cccattttgg ttccagcatc tggtcataca gacgctgaat aaccggtgcc
    14821 attttggtaa agcaggttcc tgcaaccacc atcaggtcag cctgacgcgg cgaagcacgc
    14881 aatacttctg cgccaaaacg cgccacgtca tgcaccgcgg taaacgaagt caccatctca
    14941 acgtaacagc aggaaagacc gaagttatac ggccaaattg agtttttacg accccagtta
    15001 accatgtcat tgagcttgcc cataaacacg tttttgttaa cttcttgctc cagagggtcg
    15061 gttacgatct cctgcttttg cagggggtaa cggtcgttct caccgttggg atctatgcgg
    15121 gtgagcgtat aatccatctt aatgcctcgc ggttagcgtt gacgattagc gatactgttc
    15181 gtttccgggt tcatacgctc gcggcgtgaa cgcgcgggcg tccagtccag cgcgccaata
    15241 cgcaccagat aaaccagacc tgccagtaac acaaaaataa aaattgcagc ttccacaaag
    15301 cctacccagc cgctttcgcg gatagaggtt gaccatgcga acagatacag cgcttcaacg
    15361 tcgaagataa cgaagaacat ggccaccaga taaaacttgg cggacaggcg taagcgggcg
    15421 gagccgaccg agtcgatacc ggattcaaac ggcacgtttt tcgacctcgc gcgtgcgcga
    15481 ccgcctaaaa accaaccgcc taccagcatc aggcaacaca ggccaatggc aacgataaga
    15541 aagatagcga atgcccagtg atgagcgatg acttcagtgg atgttgacat actcattgct
    15601 tactcatcaa aagtagcgcc agattcactg ctcttcacgg cagatggacg ccacatcgat
    15661 tcatggggag gaataaaaaa aaccttacaa tcactgtaga aattctttta tacagctaat
    15721 tgatgtggtc ttttactcct ttctataacc ttttgtcaac tttaacaaaa gtttcttcac
    15781 attagtttac ataatatcaa caccattagc atttaatgcc ctttcacgcc agatccttga
    15841 cgactccagg ataattagat gttgttgaat cgtgtccgtt gtgaagcaat ggaaaaaata
    15901 cgggtctatt ttgacaggaa tttgtgtcga ttcctccccc caaaagagag tattttcttg
    15961 atctgtgaca cgcttttgtc attccataac aaaaacgcag caacaaattt acgcattttt
    16021 aacatcattg tagcaggtga tttttttcag gcgattattt gtgcgttcgg gacgtgaatc
    16081 tctggtgatt gaaaaatgaa cagttttgta cgttctgcac tatgcgagga aggattttta
    16141 ctaaaaaaaa gccgctgggg tttaaaacac ccccagcggc tcgtttttac actattgtct
    16201 caggaattat ctatcgtccg tcgattactc gatatccctt tcaatcaaca atgaatcatc
    16261 cccttccgga gcagacattg ggctgtattg ccacggatta tggtagctct ccatcgcctg
    16321 atagatcacc tgggccagct cattattact ggacgggtca tagcacagca aatattcggt
    16381 atcaggtagc ggcggtaagc catctactcc actcagcaca cgcaggtacg ggctcatcat
    16441 ttcaatcggc cttgccgtca cgccaagacc ggctttcact gccgcacgaa cggccggaag
    16501 cgtcgaggcg acataagcca gtcgccatgg aatatctgct ttattaagcg tcgccagcac
    16561 catatcgcga aacggacttg gatcatccag caatacaaga gggatcggct cgcctttttg
    16621 caatacgtat tccgctgcgc agtaccagtg tgttggcgag gtacgcaggt tcaatgcctt
    16681 aaacgctgaa ggacgatggg tggttaccat caaatccact tcctgggatt ccagcatttc
    16741 tgccatatag gcattacgtt taacgcggac atccagcgct aatttcggat aaaccgaact
    16801 cacgcgattt aacaggaaag gtaagatcgt atcggcagat tcatctgaag caccgatagt
    16861 taacacgccc tgaagattac tgaacattaa tgatgagcag gcctcatcat taaaacgcag
    16921 tattttcctg gcgtaaccaa gaagttgaat gccatgttca gttaacagtt tgttgcgacc
    16981 gtgacgagcg aacagttctt tcccaacgag ttgttccaga cgctgcattt gctgacttac
    17041 ggcggactga gtacgacaca cagcggcagc tgcggcagca aaagtgttca gatcggcaac
    17101 agcaacaaat gttctcagca gatcgaggtc gaggttaatt atcggacgat ttgcacttat
    17161 catatattat cacttactgg cggctcatac tgagctggtt aatgctgtgc acacacaaac
    17221 aagcaattcc atttgtaatg tgcctccctg gcagtttcat ccggaacccg gacgaaagta
    17281 aaaatgcata tgagttgcac taaaaaagcg actcacattg ttccgttata atgcctgaag
    17341 tagatcacag aatatatctt cagggatcgc atatctatta agttactcac tcttttctat
    17401 ttatgacatg cgcgtgtttg tataaatgta aatgtgagtc cttgttccac tctcgtgcag
    17461 catcgctggt catacgcgaa cacgtacaaa cagcaatggt gaggcggcat caagagcagg
    17521 gatccgtcat ttatgcgagc attttacccc aaaaaccttt tatttataag ggtcattgcg
    17581 aattatctga tgcaaagtta tgttaggcaa agtaatcttc tattattaat aagcacatca
    17641 aaaccttttt gaatattaaa taataattaa ttagcatcat cctcattcat taattccgct
    17701 taacaatagt ttcacacctt gcccccattg cgacacctcg gaaatcatca cgctgttaaa
    17761 ctctccgtaa tgcggaaata tcatcatcgc gtttcattag tgaattctta aatgaggcat
    17821 tttcacacaa ttatcttaca gataaaaaaa ccagacttgc aattaagaat cagaacaatt
    17881 cactatataa cattgcatgc aaagcatata cgcctcatta ttttgtcatt attaagttat
    17941 taacagcaca atcgagcctt cccctctggc aaaatcttat tctgcagacc ttcaaaacac
    18001 cgtcctgggg gagtacattg ttctaagctg acttccacgg cagggagtgg cgataacagc
    18061 aaaaaaggtc aagattcatg tcccccattg aaaaatccag caaattagag aatgtctgtt
    18121 atgacatccg tggtccggtg ctgaaagaag caaaacgcct ggaagaagaa ggtaacaagg
    18181 tactgaaact gaacatcggc aacccagccc cattcggttt tgacgcgcca gatgaaatcc
    18241 tcgttgacgt gatacgcaac ctgcctaccg ctcaagggta ttgcgattcc aaagggcttt
    18301 actccgcgcg taaagccatc atgcagcact accaggctcg tggcatgcgt gatgttaccg
    18361 tggaagatat ttacatcggc aatggtgtat cggagcttat cgttcaggca atgcaggcat
    18421 tgctgaacag cggggacgaa atgttggttc ctgcaccaga ttacccactc tggaccgcgg
    18481 cggtttcgct ttccagcggt aaagcggtgc attatctttg cgatgaatcc tctgactggt
    18541 tcccggacct cgatgatatt cgcgctaaaa ttacgcctcg tacgcgtggg atcgttatta
    18601 tcaacccaaa taacccaacc ggcgcggtat attccaaaga gcttttaatg gagattgtgg
    18661 agattgcacg tcagcataat ctcattatct tcgccgatga aatttatgac aaaattctct
    18721 acgacgatgc tgagcatcac tcaattgcgc cactggcacc tgacctgctg accattacct
    18781 ttaacggact gtcgaaaacg taccgcgttg caggcttccg tcaggggtgg atggtgttga
    18841 acgggccgaa aaaacacgcc aaaggctaca tcgaaggtct ggaaatgctg gcttcaatgc
    18901 gcctgtgtgc taacgttcct gcgcaacacg ccattcagac cgcgctgggt ggttatcaga
    18961 gcatcagtga atttattacc cctggcggtc gtctttatga gcagcgtaac cgcgcgtggg
    19021 aactgatcaa cgatattccg ggcgtttcct gcgtgaaacc tcgtggtgcg ctgtatatgt
    19081 tcccgaaaat cgacgccaaa cgctttaaca ttcacgacga tcagaaaatg gtgctggatt
    19141 tcctgttgca ggaaaaagtt ctgttggtgc aagggacggc attcaactgg ccgtggccgg
    19201 atcacttccg cattgtcacg ctaccgcgtg tcgatgatat cgagctgtct ttgagcaagt
    19261 tcgcgcgttt cctttctggt tatcatcagc tgtaatctta atttcactgc cggagattgc
    19321 atccggcagc gttatcccgc cacaatgacc tgatgatgtc atcatacgta aggtcactat
    19381 gaaacagagc catttttttg cccatctctc ccgcctgaaa ctcattaacc gctggccgct
    19441 aatgcgcaac gtgcggacgg aaaatgtgtc cgaacacagt ttgcaggtag cgatggtcgc
    19501 ccatgcgctg gcagctatca aaaatcgaaa atttggcggt aatgtcaacg ccgaacgtat
    19561 cgctttactg gcgatgtacc acgatgcctc agaagtgctc accggcgatc tccctactcc
    19621 ggtgaaatac ttcaattcgc aaatcgctca ggagtacaag gctattgaaa aaatcgctca
    19681 gcaaaaactg gtcaatatgg ttccggaaga gctgcgggat atctttgcgc cgttaattga
    19741 cgagcatgca tatagcgatg aagaaaaatc gctggtgaaa caggcagatg cactgtgtgc
    19801 atatctgaaa tgtctggaag aactcgcggc cggaaataat gaattcttgc tggcaaaaac
    19861 gcgactggaa gcgacgcttg aagcgcgtcg cagccaggag atggactact tcatggaagt
    19921 atttgttccc agcttccatc tttcgctcga tgagattagc caggattcac cgctgtaagc
    19981 agccggagtc tgcgtcgcat caggcaataa gcgccggatg cgacatcagg ctcttgtcaa
    20041 aacggaaaca gcatcgggat catcaccaca caaaccgcca tcacgataat ggtgaacggt
    20101 acccccaact tcacaaagtc actgaagctg taatttcccg gacctaaaac cagtgtgtta
    20161 acaggtgaag aaaccggtgt cataaaggcg gcggatgctg ccatcgccac gaccatcgcg
    20221 aatggataag gcgacacccc catcgttttg gcagcagcca gcgcaatcgg agccatcaac
    20281 accgccgtcg cggtattaga gataaatagc ccaataaccg ccgacaagac aaacaaacac
    20341 cccagcatca tatgtggccc gtaaccaccg ccaatgtcca tcagcccttt caccgccagc
    20401 gcgacacctc ccgttttctg taatgccaca gcaaacggca tcatcccaac gatcaaaata
    20461 atgctcggcc agtgaatgga tttataggcg ctttcagcat ctatacagcg gaatttcccc
    20521 atcagcaggc aggcgatgat agcggcaaca ggattaggaa tttcatctgt cagcattaac
    20581 gccaccatta gcaccagaca gaaaatggca tggggtgcct ggctgtgcgc gggcgatgct
    20641 tcactcacct cttccggtaa gttcagcgct acgaagtcgc ggccctgttt ggccagcata
    20701 ccgatcagtt tccagttacc cacaaccagg atgatatcgc ccagcagcag aggctcatcc
    20761 gccagcgaac cttccagcgc cacgccattg cgctttagcc ccaccacatt cagtccgtag
    20821 cgggtacgaa aaccaatttc gcgcaccgat ttaccaatca gttctgactc aggaattaat
    20881 gaaatctctg ccatgcccac atcaagggcc tggtcagaaa aatactcgcc gcgcagtacc
    20941 atcggctcca gcaattgctc actacaaaat tgccggagat cgacatcagc cgcagacata
    21001 tcaataagca aaacgtcacg cgcgcgaaat tcagaaaccc cattaacgtt cacgataacg
    21061 cgacgaaaac gccgccagcg ttcaacaccg atgacgttag cgccataacg ctcacgtaat
    21121 ttgagatcat ccagccgttg accaatcatt ggcgatccgg ggcgaatagc cagacgtcgc
    21181 gcacgcccgg tcagtcgata ttcacggata agatcgcgaa aggttcgacg cgtccagcct
    21241 tcgcgctgcg gggtctgggt atcccctttc agcatgaaac gcatcactaa catatacaaa
    21301 atacccagca ccagcacgac caggccaata ggtgttacgc taaagaaact gaagccgtga
    21361 tagccttcac gcagcaattc actgttgact accaggttcg gcggcgtcgc caccagcgtc
    21421 atcatgccgc ttatcagccc ggcaaaactc aacggcatca tcagacgtga cggcgaagtt
    21481 tgcatacgca tggcaacgct taacaccacg ggaataaaga tagcgacaac gccggttgaa
    21541 ctcataaacg cgccaagccc cgcgacggtc agcatcaaca aaaccaacat tttgatttca
    21601 ctgttgcccg caactttgac cagccatgtt cccattacgg tggcaacacc ggtacggacc
    21661 aaaccatcgc caataataaa caaggcggca atcaggacaa cgttaggatc agaaaagccg
    21721 gaaaatactt cggggactgt cagcgttccg cttaatgcaa acgcgacaat aacaaacaaa
    21781 gcgaccgcat ccatacgcac tctgcccgtc gcaaacaaga caatggcaac ggccagtaat
    21841 gaaagaaccc aaatcaattc accgttcaca acttatcctt gttaattgag ggagatgtct
    21901 tgattctgcc ataaaaaagc cccgacgaga cggggctaaa tcatgatcag gtgtttcact
    21961 gaataataac atcgccattt ggctgtttgg tcacagtaat ttgctccaga ctgtggagga
    22021 ccaaatcgac ctcattcagg cgcggggtat ctgccggagc gttaaccgca atgacatgac
    22081 aacccgccgc caggccagaa agcacgccag cggagcatct tccaccacca cacactcctg
    22141 cggcgcaagc cccagcagct gcgcgcctaa cagatacgca tcaggttctg gttttccgcg
    22201 cttcactcgc tcagcggtta caaacacctc tggtgcggga agcccagcta ttttatggcg
    22261 cgctcgcgct accggcatgg agccagaagt cacaatggcc cacggaatac ctgctttatt
    22321 caaatgactg agtaaggcga ttgcccccgg aagcgggtaa taccttcggt ttccgtggcc
    22381 tcgatgtgct ccagacgcgt aaactcggcg gcaatatcag cctcggattt gcccgccata
    22441 aaatggcgca gagaggtgat cgcctgttta ccgtgaatga aagccagcac ctcttccggc
    22501 gctaacccat gacgtctggc ccagttgctc cacgcccgtt ctaccgcagg cagggaatcc
    22561 accagcgttc catcaagatc aaacagaaaa cctttgcacc gcacgcgggc ctcctcaggc
    22621 attgataatt tgattaattt cgttggcgct caaatggtac tgacgcgggc aggcatgcca
    22681 cacattaagc atgcgctgat atttttccca cattggcgtc tgggcgttaa aaccgtgagt
    22741 tccggcgtca aaatgggtat agcgcccttc cacattaacc ataaagcgga cataaccgag
    22801 gtaacgtgct tcagtggcgg cgtcaaagcc gaggaaggtg acacgacgtt catcgatgga
    22861 ttgctggtcc tgcaaattag accaggaaac atgcaacgca tgatacatct ccataatgtc
    22921 gatgatagtg cggcaggttt cttctttcag ctcgccaaac tcgcgatcca attcacgcat
    22981 ctgtaatccg taacctcgct caataattgt ttgcaggcga cggtaacgtt cagcatttgc
    23041 cggatcgagc atagtcatca tcttgtactg attagacaaa ataagacgtt gcgcgttggt
    23101 catttccatt gttgactcct gtatcactct actacggtga aaaaaaagaa ggctgagtat
    23161 gccttctttt atatgcgtaa tcaggggtca attacaaatc atcaaggaaa gttttatcca
    23221 gttgtttgaa ggcgcgctta agcgtgtcag ctaatgcctg gtaatcaggc ttgccttcaa
    23281 cgggtgccaa cacctgtcca gactcctgca atttaccgcg aacttcataa aaccagttaa
    23341 ggattgcagg gggtaatggc gttacagaac gcttgcccag ccaccacaat ccctgcatgg
    23401 gtaaacttaa ggcgaacagt gcagtggcaa ctgccggccc aagctgaccg cccagggcaa
    23461 tctgccagca gagagtaaat acggcgatcg gcggcataaa acggatcgca taacgcgtca
    23521 tcttgataac gcgattttcg acaaagaccg gggcaaggcg tttttccagt ggccacgtct
    23581 ttgagtaatg ctgtccgcgg cgaaacaagc taaaaaaatt aacagaacga ttatccggcg
    23641 ttgacatgct tcacctcaac ttcacatata aagattcaaa aatttgtgca aattcacaac
    23701 tcagcgggac aacgttcaaa acattttgtc ttccataccc actatcaggt atcctttagc
    23761 agcctgaagg cctaagtagt acatattcat tgagtcgtca aattcatata cattatgcca
    23821 ttggctgaaa attacgcaaa atggcataga ctcaagatat ttcttccatc atgcaaaaaa
    23881 aatttgcagt gcatgatgtt aatcataaat gtcggtgtca tcatgcgcta cgctctatgg
    23941 ctccctgacg tttttttagc cacgtatcaa ttataggtac ttccatgtcg agtaagttag
    24001 tactggttct gaactgcggt agttcttcac tgaaatttgc catcatcgat gcagtaaatg
    24061 gtgaagagta cctttctggt ttagccgaat gtttccacct gcctgaagca cgtatcaaat
    24121 ggaaaatgga cggcaataaa caggaagcgg ctttaggtgc aggcgccgct cacagcgaag
    24181 cgctcaactt tatcgttaat actattctgg cacaaaaacc agaactgtct gcgcagctga
    24241 ctgctatcgg tcaccgtatc gtacacggcg gcgaaaagta taccagctcc gtagtgatcg
    24301 atgagtctgt tattcagggt atcaaagatg cagcttcttt tgcaccgctg cacaacccgg
    24361 ctcacctgat cggtatcgaa gaagctctga aatctttccc acagctgaaa gacaaaaacg
    24421 ttgctgtatt tgacaccgcg ttccaccaga ctatgccgga agagtcttac ctctacgccc
    24481 tgccttacaa cctgtacaaa gagcacggca tccgtcgtta cggcgcgcac ggcaccagcc
    24541 acttctatgt aacccaggaa gcggcaaaaa tgctgaacaa accggtagaa gaactgaaca
    24601 tcatcacctg ccacctgggc aacggtggtt ccgtttctgc tatccgcaac ggtaaatgcg
    24661 ttgacacctc tatgggcctg accccgctgg aaggtctggt catgggtacc cgttctggtg
    24721 atatcgatcc ggcgatcatc ttccacctgc acgacaccct gggcatgagc gttgacgcaa
    24781 tcaacaaact gctgaccaaa gagtctggcc tgctgggtct gaccgaagtg accagcgact
    24841 gccgctatgt tgaagacaac tacgcgacga aagaagacgc gaagcgcgca atggacgttt
    24901 actgccaccg cctggcgaaa tacatcggtg cctacactgc gctgatggat ggtcgtctgg
    24961 acgctgttgt attcactggt ggtatcggtg aaaatgccgc gatggttcgt gaactgtctc
    25021 tgggcaaact gggcgtgctg ggctttgaag ttgatcatga acgcaacctg gctgcacgtt
    25081 tcggcaaatc tggtttcatc aacaaagaag gtacccgtcc tgcggtggtt atcccaacca
    25141 acgaagaact ggttatcgcg caagacgcga gccgcctgac tgcctgattt cacaccgcca
    25201 gctcagctgg cggtgctgtt ttgtaacccg ccaaatcggc ggtaacgaaa gaggataaac
    25261 cgtgtcccgt attattatgc tgatccctac cggaaccagc gtcggtctga ccagcgtcag
    25321 ccttggcgtg atccgtgcaa tggaacgcaa aggcgttcgt ctgagcgttt tcaaacctat
    25381 cgctcagccg cgtaccggtg gcgatgcgcc cgatcagact acgactatcg tgcgtgcgaa
    25441 ctcttccacc acgacggccg ctgaaccgct gaaaatgagc tacgttgaag gtctgctttc
    25501 cagcaatcag aaagatgtgc tgatggaaga gatcgtcgca aactaccacg ctaacaccaa
    25561 agacgctgaa gtcgttctgg ttgaaggtct ggtcccgaca cgtaagcacc agtttgccca
    25621 gtctctgaac tacgaaatcg ctaaaacgct gaatgcggaa atcgtcttcg ttatgtctca
    25681 gggcactgac accccggaac agctgaaaga gcgtatcgaa ctgacccgca acagcttcgg
    25741 cggtgccaaa aacaccaaca tcaccggcgt tatcgttaac aaactgaacg caccggttga
    25801 tgaacagggt cgtactcgcc cggatctgtc cgagattttc gacgactctt ccaaagctaa
    25861 agtaaacaat gttgatccgg cgaagctgca agaatccagc cctctgccgg ttctcggcgc
    25921 tgtgccgtgg agctttgacc tgatcgcgac tcgtgcgatc gatatggctc gccacctgaa
    25981 tgcgaccatc atcaacgaag gcgacatcaa tactcgccgc gttaaatccg tcactttctg
    26041 cgcacgcagc attccgcaca tgctggagca cttccgtgcc ggttctctgc tggtgacttc
    26101 cgcagaccgt cctgacgtgc tggtggccgc ttgcctggca gccatgaacg gcgtagaaat
    26161 cggtgccctg ctgctgactg gcggctacga aatggacgcg cgcatttcta aactgtgcga
    26221 acgtgctttc gctaccggcc tgccggtatt tatggtgaac accaacacct ggcagacctc
    26281 tctgagcctg cagagcttca acctggaagt tccggttgac gatcacgaac gtatcgagaa
    26341 agttcaggaa tacgttgcta actacatcaa cgctgactgg atcgaatctc tgactgccac
    26401 ttctgagcgc agccgtcgtc tgtctccgcc tgcgttccgt tatcagctga ctgaacttgc
    26461 gcgcaaagcg ggcaaacgta tcgtactgcc ggaaggtgac gaaccgcgta ccgttaaagc
    26521 agccgctatc tgtgctgaac gtggtatcgc aacttgcgta ctgctgggta atccggcaga
    26581 gatcaaccgt gttgcagcgt ctcagggtgt agaactgggt gcagggattg aaatcgttga
    26641 tccagaagtg gttcgcgaaa gctatgttgg tcgtctggtc gaactgcgta agaacaaagg
    26701 catgaccgaa accgttgccc gcgaacagct ggaagacaac gtggtgctcg gtacgctgat
    26761 gctggaacag gatgaagttg atggtctggt ttccggtgct gttcacacta ccgcaaacac
    26821 catccgtccg ccgctgcagc tgatcaaaac tgcaccgggc agctccctgg tatcttccgt
    26881 gttcttcatg ctgctgccgg aacaggttta cgtttacggt gactgtgcga tcaacccgga
    26941 tccgaccgct gaacagctgg cagaaatcgc gattcagtcc gctgattccg ctgcggcctt
    27001 cggtatcgaa ccgcgcgttg ctatgctctc ctactccacc ggtacttctg gtgcaggtag
    27061 cgacgtagaa aaagttcgcg aagcaactcg tctggcgcag gaaaaacgtc ctgacctgat
    27121 gatcgacggt ccgctgcagt acgacgctgc ggtaatggct gacgttgcga aatccaaagc
    27181 gccgaactct ccggttgcag gtcgcgctac cgtgtttatc ttcccggatc tgaacaccgg
    27241 taacaccacc tacaaagcgg tacagcgttc tgccgacctg atctccatcg ggccgatgct
    27301 gcagggtatg cgcaagccgg ttaacgacct gtcccgtggc gcactggttg acgatatcgt
    27361 ctacaccatc gcgctgactg cgattcagtc tgcacagcag cagtaacctc gtcatcatcc
    27421 gcagctttgc gctgcggata tctgaaccgg aaataatcac tatttccggt tttttattct
    27481 cttaatctgc attaatcctt tctgattatc ttgcttaact gcgctgcatc aatgaattgc
    27541 gccatttcac tttacatact taccactttg ttttgtgcaa gggaatattt gcgctatgtc
    27601 cgcaatcact gaatccaaac caacaagaag atgggcaatg cccgatacgt tggtgattat
    27661 cttttttgtt gctattttaa ccagccttgc cacctgggta gttccggtgg ggatgtttga
    27721 cagtcaggaa gtgcagtatc aggttgatgg tcaaacaaaa acacgcaaag tcgtagatcc
    27781 acactcattt cgcattctga ctaacgaagc aggcgaacct gagtatcacc gcgtacagct
    27841 gttcacgacg ggcgatgaac gcccgggcct gatgaacttc ccgtttgaag gattaacctc
    27901 aggatcgaaa tacgggacag ctgttggcat catcatgttt atgctggtga ttggcggcgc
    27961 gtttggcatt gtgatgcgta caggaaccat tgataacggt atcctggcgc ttattcgcca
    28021 tacccgcggg aatgaaattc tctttattcc tgcgctgttt attctgtttt cacttggcgg
    28081 cgcggtattt ggtatgggag aagaggccgt cgcctttgcc attatcatcg caccgctaat
    28141 ggtccggctg ggctatgaca gtattaccac cgtcctggtg acctatattg ccacgcaaat
    28201 cggttttgcc agttcgtgga tgaacccgtt ttgtgtggtc gttgctcagg ggattgccgg
    28261 cgttccggtg ctttctggct ccgggttgcg catcgtggtg tgggttatcg ccactatgat
    28321 tggcctgatc tttaccatgg tgtacgcctc acgagtgaaa aagaatcctc ttctgtcacg
    28381 cgtgcatgag tccgaccgct tctttcgtga aaagcaggcg gatgttgaac aacgtccgtt
    28441 tacctttggt gactggctgg tattgattgt cctgaccgcc gtaatggtct gggtgatttg
    28501 gggcgtgatc gttaatgcct ggtttattcc agaaattgcc agccagttct tcaccatggg
    28561 tctggtgatt ggcatcatcg gcgttgtttt ccgccttaac ggcatgacgg ttaataccat
    28621 ggcttcatcc tttaccgaag ggacgcgaat gatgatcgcc cctgccctgc tggtgggttt
    28681 cgccaaaggg attttgctgc tggtcggtaa tggtgaagcg ggtgatgcca gcgtgttaaa
    28741 taccatcctc aacagcattg ccaatgccat tagcggtctg gacaacgcgg tcgcggcctg
    28801 gtttatgttg ctcttccagg cggtatttaa tttcttcgtg acgtccggtt ctggtcaggc
    28861 ggcgttaacc atgccgttac tggcaccgct tggcgatctg gtcggtgtta accgtcaggt
    28921 taccgtgctg gcattccagt ttggtgatgg cttcagtcac atcatttacc caacctcagc
    28981 ttcgttaatg gcaactctcg gtgtgtgcag ggtggacttc cgtaactggc tgaaggtggg
    29041 cgcgacccta cttggactgc tgtttattat gtccagcgtc gtagtgatcg gcgctcagtt
    29101 gatgggctac cactaaaaat gttaagagcc gcaattgcgg ctcttttttc attctgccgt
    29161 ttcagtctct acggcttcat ttttggcatt gcgcttcatc cacaacgcca gcgctttcag
    29221 cgagtctgga gtgaactcat cgcagcgtgc ggtgatttct tccggcgtca gccagcaaac
    29281 ttcactgact tcatcttcct gtagggcgaa gggaccgtga gagacgcagc tgaacaacgc
    29341 gccccagaca cggcaatttt tatcttcgaa atagaactgc ccgtgctcgg caaaggggac
    29401 accggcaatg cccaactctt cttccgcttc gcgacgcgcg gattccagca gttgctcatc
    29461 ggcctgaacc acaccgcctg cggtcgcatc taacatgccg ggtaaaaagt cttttgtctc
    29521 ggtacgacgc tggaccagaa ttttgcccat gccatcatgc acgacgatgt aagttgcacg
    29581 atgacgcaga cactgtgccc gcatttgttc ccggctggct tgtgcaatga cttcgttctc
    29641 ttcattgaca atatccaccc attcagtact tgccaaacga cgctgttcca ttatcaggaa
    29701 accttctttt tctggcgctc ttacggcgca ttttgagttg tgggtaaatt acggattaat
    29761 cgcgacctgc gcaatgatac tttgatcatt gagtgcgatt acactaagta cgtcattatc
    29821 cagcatgcca taactcgccg gattaccgcc tttcggaata ctcaccgagc cggggttgaa
    29881 gtggaaaatc tccccccgct gttctgccac tggcagatgg gtatgaccgt acaccagcac
    29941 atcgttctgg tttaaagcag gtagattttc cgggccaaaa agatgaccat gcgtcaaaaa
    30001 cagacgttgt ttttccagta atacctgttg ccacggcgcg gttatcggga aatgcagcag
    30061 catttgatcc acttcgctgt cgcagttgcc gcgcacagcg ataaccttat gtgccacttc
    30121 attaagccgt tcagcgactt tggctggcgc gtaaccctcc ggtaaagcat tacgcgggcc
    30181 gtgattcaac acgtcgccaa ggatcaccag ccactgggca ccgctttggg caaacaactc
    30241 cagaacacgt tccgtcgccg gtaacgaccc atgaatgtcc gatgcaaaca tcagtttcat
    30301 cactcactcc tcgtcgaaaa aacgtaagct atgatactgg attctgtcgt tgctatcagc
    30361 cagattgctt cgccgaaagt gcaataaaac gctggacaga cgctcgctgc cactggaatg
    30421 tcgcataatc caccaggcgt tctggcacct catcgccatg tagtaccagg cggttaatca
    30481 ttagcgccag atcagtatca gcaatgcacc attcaccaaa taaattcggc tgacccagtg
    30541 ctaacaaatg ttctgccatc gcgaacagtt tctctgcact ggcttttccc tcggccgtta
    30601 gtggcgcttt tttcgccccc gcaaagacaa catccgtcgg acgctcttcg cggatgggca
    30661 tcagatcgct gcgcagccag gcctgaatcc gtcgtgcacg cgcacgattt tctaaatcaa
    30721 gcggataaat acgttcccag gtcggtggcg caaatcgatc ttccagatac tccgcaatgg
    30781 cagaagattc actcaactca aaatcatcga tttgtaataa cggcacacgg cgtgtctgac
    30841 cgtaaccttg ccacgtcggc ttcaaatgtt caccgctgtc gaggtcgatg gtcttgatat
    30901 gaaacgacag gcctttttcc tgcaacgcca cccaggcgga taacacataa ggggagaaaa
    30961 agtgggcatc tgaccaaagc gtgatagcgg gtttactcat tatgtcctcg gcaataaagg
    31021 gggctggcta tcaaactata gagccttttt gtcactgtca cgtgacgaat actcacgtaa
    31081 gtcgccgcgc tttctatact tgtggtgttc agatgattaa acaaccggag ctgcaatgat
    31141 cgatctctat ttcgccccga cacccaatgg tcacaaaatt acgctgtttc tcgaagaagc
    31201 agagcttgat tatcgcttga ttaaggtaga cctggggaaa ggcggtcagt ttcgtccgga
    31261 atttttgcgc atttcgccca acaacaaaat tccggcaatt gttgatcatt ctcctgccga
    31321 tggcggcgaa ccgctaagcc tctttgagtc tggtgccatt ttgttgtatc tgactgagaa
    31381 aacaggactc tttttgagtc atgaaacgcg tgagcgcgcc gccacattac agtggttatt
    31441 ctggcaggta ggcggactgg ggccgatgct tgggcaaaat catcatttta atcacgcagc
    31501 cccccaaacc attccttacg ctattgaacg ttatcaggtt gaaactcagc gtctttacca
    31561 tgtactaaac aagcggctgg aaaactcgcc ctggctggga ggcgagaact acagcattgc
    31621 ggatattgcc tgctggccgt gggttaatgc ctggactcgc cagcgaattg acctcgcaat
    31681 gtatccggca gtcaagaact ggcatgagcg gatccgttcg cgccctgcca ccgggcaggc
    31741 actgctaaaa gcacaactcg gtgatgagcg ttcggatagt taacagaaac aggttctcgt
    31801 gtattatttc atcctaagta aaacaacgga gaacctgcaa tggcacaacc tgccgctatt
    31861 attcgtataa agaaccttcg tttacgtacg tttatcggaa ttaaggaaga agaaattaac
    31921 aaccgtcagg atattgttat caatgtgacg atccactacc ccgccgataa agcgcgcact
    31981 agcgaagata tcaacgatgc gctgaattat cgcaccgtaa cgaaaaacat tattcagcat
    32041 gtagagaata accgtttctc tttgttggaa aaattaactc aggatgtgct cgatatcgca
    32101 cgtgaacatc actgggtgac gtatgctgaa gtggagatcg ataaactgca cgcgctgcgc
    32161 tacgccgatt cggtatccat gaccttaagc tggcagcgtt aaccgccata tcgggaggct
    32221 gcatgaacat agtgatcacc ggagggacgg gattaattgg tcgccatttg attccgcgtt
    32281 tgctggagct gggccatcaa atcacggtag tgacgcgtaa cccacagaaa gccagttccg
    32341 ttctcggccc tcgggtgacg ctatggcaag ggctagccga tcaaagcaac ctcaacggcg
    32401 ttgatgcggt aatcaacctg gccggagaac cgattgctga taaacgctgg actcacgagc
    32461 aaaaagagcg tctctgccaa agccgctgga atatcacgca aaaactggtc gatttgatta
    32521 atgccagcga cacgccgccg tcggtattca tttccggctc ggcaacgggc tattatggcg
    32581 acttaggtga agtggtggtt accgaagagg aaccgccgca taacgaattt actcataaac
    32641 tctgcgcccg ctgggaagag attgcctgcc gggcgcaaag tgacaaaacg cgagtgtgcc
    32701 tgctgcgaac cggcgtggta ctggcaccgg acggcggtat tctcggtaaa atgctgccac
    32761 cgtttcgtct tggcctgggc gggccgattg gttccggtcg gcagtatctg gcctggattc
    32821 atattgatga tatggtcaac ggcattctct ggctgctgga taacgacctg cgcgggccat
    32881 ttaatatggt ttcgccctac ccggtacgca atgaacaatt tgcccatgcg ctcggtcaag
    32941 cgctgcatcg cccggccatt ttgcgcgtcc ccgcaaccgc cattcggctg ttaatgggcg
    33001 aatcttcaat actggtatta ggtggacaac gcgcgctgcc taaacggctg gaagaagcgg
    33061 gttttgcgtt tcgctggtac gatttagaag aggcgctggc ggatgtcgtt cgctgatgtg
    33121 gtttacagca aacatccgcc agttaactcc cggtgttaca ggattagtgg ctttgcgcga
    33181 taagatcgtc tggtgaaagt cgggtcacca tcataactaa ctctctgtct aaacctctat
    33241 ccagcatctc ctgagcaata cgcagggctt cttcgtgttt gccctgcatt gcgccttctt
    33301 cacgtaatct gtcagcaatg gtcatcagtt tctccttttc ttgtggtgcg cgttctgtta
    33361 tctcaccaat aaatgcacgg aaacgctggg catcccctgt ttgtaatacg taattaaaca
    33421 gggcttttag ctgtctgtca ttagtgttcc ctgtaactag cagcgaaaca atttggtcga
    33481 ctaatcccaa cagatcgcgc tgacgaatat gtttctgaat taactccaac agcgccattt
    33541 tgcggtgttg cataatctcg tcatccggca ccacggtaat atccaccaac ggaaacgctg
    33601 atgaatatat tttgcgggct atagcaggct cggcaaattc atcaagccag cagagtgaat
    33661 aaggataagg acttctgcaa ccatgataaa acagcatcgg gatcaccaat ggaagctctt
    33721 tatagcccgc atcaagatgg ttttgcattg ccgcaatgga ataacgcat
//
LOCUS       DAJVQG010000029        32648 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJVQG010000029 DAJVQG010000000
VERSION     DAJVQG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32648
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.029"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            74..1732
                     /locus_tag="OQM83_001254"
     CDS             74..1732
                     /locus_tag="OQM83_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCS1519555.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1729..2685)
                     /locus_tag="OQM83_001255"
     CDS             complement(1729..2685)
                     /locus_tag="OQM83_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCS1519556.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2836..3951
                     /gene="macA"
                     /locus_tag="OQM83_001256"
     CDS             2836..3951
                     /gene="macA"
                     /locus_tag="OQM83_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCS1519557.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            3948..5894
                     /gene="macB"
                     /locus_tag="OQM83_001257"
     CDS             3948..5894
                     /gene="macB"
                     /locus_tag="OQM83_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCS1519558.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(5967..6191)
                     /gene="cspD"
                     /locus_tag="OQM83_001258"
     CDS             complement(5967..6191)
                     /gene="cspD"
                     /locus_tag="OQM83_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCS1519559.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6514..6834
                     /gene="clpS"
                     /locus_tag="OQM83_001259"
     CDS             6514..6834
                     /gene="clpS"
                     /locus_tag="OQM83_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCS1519560.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6865..9141
                     /gene="clpA"
                     /locus_tag="OQM83_001260"
     CDS             6865..9141
                     /gene="clpA"
                     /locus_tag="OQM83_001260"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCS1519561.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9547..9634)
                     /locus_tag="OQM83_001261"
     tRNA            complement(9547..9634)
                     /locus_tag="OQM83_001261"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9598..9600),aa:Ser,seq:gga)
     gene            complement(9888..10106)
                     /gene="infA"
                     /locus_tag="OQM83_001262"
     CDS             complement(9888..10106)
                     /gene="infA"
                     /locus_tag="OQM83_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCS1519562.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10391..11095)
                     /gene="aat"
                     /locus_tag="OQM83_001263"
     CDS             complement(10391..11095)
                     /gene="aat"
                     /locus_tag="OQM83_001263"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCS1519563.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(11137..12858)
                     /gene="cydC"
                     /locus_tag="OQM83_001264"
     CDS             complement(11137..12858)
                     /gene="cydC"
                     /locus_tag="OQM83_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCS1519564.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGGAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(12859..14625)
                     /gene="cydD"
                     /locus_tag="OQM83_001265"
     CDS             complement(12859..14625)
                     /gene="cydD"
                     /locus_tag="OQM83_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCS1519565.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14748..15713)
                     /gene="trxB"
                     /locus_tag="OQM83_001266"
     CDS             complement(14748..15713)
                     /gene="trxB"
                     /locus_tag="OQM83_001266"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCS1519566.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLHDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16258..16752
                     /gene="lrp"
                     /locus_tag="OQM83_001267"
     CDS             16258..16752
                     /gene="lrp"
                     /locus_tag="OQM83_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCS1519567.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            16887..20954
                     /gene="ftsK"
                     /locus_tag="OQM83_001268"
     CDS             16887..20954
                     /gene="ftsK"
                     /locus_tag="OQM83_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCS1519568.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21109..21720
                     /gene="lolA"
                     /locus_tag="OQM83_001269"
     CDS             21109..21720
                     /gene="lolA"
                     /locus_tag="OQM83_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCS1519569.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21731..23074
                     /gene="rarA"
                     /locus_tag="OQM83_001270"
                     /gene_synonym="mgsA"
     CDS             21731..23074
                     /gene="rarA"
                     /locus_tag="OQM83_001270"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCS1519570.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23165..24457
                     /gene="serS"
                     /locus_tag="OQM83_001271"
     CDS             23165..24457
                     /gene="serS"
                     /locus_tag="OQM83_001271"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCS1519571.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24696..27140
                     /gene="dmsA"
                     /locus_tag="OQM83_001272"
     CDS             24696..27140
                     /gene="dmsA"
                     /locus_tag="OQM83_001272"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HCS1519572.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27151..27768
                     /gene="dmsB"
                     /locus_tag="OQM83_001273"
     CDS             27151..27768
                     /gene="dmsB"
                     /locus_tag="OQM83_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCS1519573.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27770..28633
                     /gene="dmsC"
                     /locus_tag="OQM83_001274"
     CDS             27770..28633
                     /gene="dmsC"
                     /locus_tag="OQM83_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCS1519574.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28669..29295)
                     /locus_tag="OQM83_001275"
     CDS             complement(28669..29295)
                     /locus_tag="OQM83_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1519575.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29610..30758
                     /locus_tag="OQM83_001276"
     CDS             29610..30758
                     /locus_tag="OQM83_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1519576.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            30968..32398
                     /locus_tag="OQM83_001277"
                     /pseudo
     CDS             30968..32398
                     /locus_tag="OQM83_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(32399..>32648)
                     /locus_tag="OQM83_001278"
     CDS             complement(32399..>32648)
                     /locus_tag="OQM83_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1519577.1"
                     /translation="DDVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITER
                     PGFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         8002 a         8003 c         8776 g         7867 t
ORIGIN      
        1 catcacgtat tagcaaggcc tttcccgtta tactgccagc gtaaaggata agtcacatat
       61 ttctggaggg gatatgattc ttgagcgcgt tgaaattgtg ggttttcgcg gtatcaaccg
      121 tttgtcgttg atgctggaac aaaacaacgt cctgattggg gagaacgcgt ggggtaaatc
      181 cagcttgctg gacgccttaa ccctgctgct atcgccagaa tcagatctct accattttga
      241 gcgcgacgat ttctggttcc cgccgggaga tatcaacggg cgagaacatc atctgcatat
      301 tattttgacc ttccgcgaat cgctgccagg ccgacatcgg gttcgccgtt atcggccgct
      361 ggaagcgtgc tggacgccat gcaccgatgg ctatcaccgt attttttatc gtctggaagg
      421 ggagagtgcg gaagacggca gcgtgatgac actgcgcagt tttctcgata aagacggaca
      481 tccgattgat gtcgaggata ttaacgatca ggcacgccat ctggtgcgtt taatgccggt
      541 gctgcgcttg cgtgatgccc gttttatgcg ccgtattcgt aacggcacgg tgccaaatgt
      601 ccctaatatg gaagtcaccg cgcgccagct cgatttcctc gcccgtgagt tatcctcaca
      661 tccgcaaaat ctctctgatg ggcagattcg tcagggactt tccgcaatgg tacagctgct
      721 tgagcattat ttctctgagc agggggccgg acaggcgcga tatcgtttaa tgcggcggcg
      781 agccagcaat gagcaacgaa gctggcgcta tctggatatc atcaaccgga tgattgaccg
      841 acctggtggg cgctcgtatc gggttatttt gctcggcctg tttgctactt tgttgcaggc
      901 aaaaggcaca ttgcgactgg ataaagacgc ccgtccattg ttgctgatcg aagatccaga
      961 aacccgttta caccccatta tgctttcagt tgcctggcat ctgttgaatc ttctgccatt
     1021 gcagcgcatt gtcaccacca actcgggtga gttgctttcg ttaacgccgg tagagcatgt
     1081 ttgccgactg gtacgtgagt cctcgcgcgt tgccgcctgg cgtctggggc cgagtggctt
     1141 gagtaccgaa gatagccgac gcatatcctt tcacattcgt tttaaccgtc cgtcatcgct
     1201 gtttgcacgc tgctggttgc tggtggaagg ggaaacggaa acctgggtta tcaatgaact
     1261 ggcgcgtcag tgcggacatc attttgatgc cgaagggatc aaggtcattg agtttgccca
     1321 gtccgggcta aagccactgg ttaaatttgc ccgccgaatg gggattgaat ggcatgtact
     1381 ggtcgatggc gatgaagcag ggaagaaata tgccgctacg gtacgcagcc tgttgaataa
     1441 cgatcgggaa gccgaacgag aacatttaac ggcgttaccg gcgctggata tggaacattt
     1501 tatgtatcgc cagggatttt ccgatgtgtt ccaccgcgtg gcgcaaatcc cggaaaatgt
     1561 accgatgaat ctacgcaaaa ttatctcgaa agcgatccat cgctcttcca aacccgatct
     1621 tgccattgaa gtggcaatgg aggcaggacg tcgtggtgtg gactccgtac cgacgctgct
     1681 gaaaaaaatg ttctcacgcg tgctgtggct ggcgcgcggt cgcgcggatt aaccgcgaaa
     1741 catcgtggcc atttgtggct gaatagcgtc gagcatctca tagcgccgac ggtattcagc
     1801 ccgtttttta ctggcgattt cggcaatctc ttttcgtgct atctgtgctg gaaggcggta
     1861 atggcgttca gcatcacata cgccgccaac cgattcccag aaagcgttgt aatcagcgtg
     1921 gatcttgcct tctttatcgc gataacgcag gctgcggtaa atatgcgttt cattgctgac
     1981 ggcaataatc tgctctacct gcaaacgttg ggcaaacaga caggccgctt ccatcacgag
     2041 gcgtttggga aatagcccgt ggcaggcttt cgtcgcattc tggatttcct gatgtggaat
     2101 ttcccatttt gcgccttgca gtccgccaat aaacatcgtt cttttcccct gatattcaca
     2161 cagggtaaac gtgatctctg ccagaggaat accttcgctg ttgcgaaaca ggattgtgct
     2221 gtcaccttct ttatccattg agatcatcat ggtcagctca agcgtgaact gctcgccgtt
     2281 tttgccttcc agcttcgcca gttgcagccc gggggtattc aaatataagc tgaattcttc
     2341 cgccgacata catccgcgga gtaacgcata atggtaacgt aacgcctcca gcaattgctt
     2401 acggctaaga ttcgccgcaa ggtaagggcg atgcagacgc acaggcagtc gcggttggcg
     2461 cgttaacaac acattgagat taggccagtg ggaaagttcg ttcatccact caacgcttaa
     2521 acgcggcata atcaacgagc gcagcaaaaa tttctggcga aaactacggc gatgccagaa
     2581 tttacccggc cgacactgtc cacgtgccag actaagaaaa agtgacaggc tgctgagaga
     2641 ttcagatggc gtaaaggtcc gttcagttag ctgcgacata ttcatgaaat caatggttat
     2701 acatgacgtc gatttcacca ttgcgtatct taaccaaaca tcaatagtgt gattactaac
     2761 gtaaatttta tggttttgtt gatatttcgt tgaagttaat gacccggatt ggcatatgga
     2821 gtattcagaa tatttatgaa aaagcggaaa accgtgaaga agcgttacgt tattgcgctg
     2881 gtgatagtca tcgccggact gattacgtta tggagaattc ttaacgcacc cgtgccgact
     2941 tatcagacac tgattgtgcg ccccggtgat ttacagcaaa gcgtgctggc gaccggaaag
     3001 ctggacgcgc tgcgtaaggt tgacgtgggc gcgcaggtca gcggtcagtt gaaaacgctg
     3061 tcggtggcga ttggcgataa agtaaaaaaa gaccagcttt taggggtcat tgatcctgaa
     3121 caggctgaaa accagatcaa ggaggtcgaa gcaacgctga tggagctacg cgcgcagcgg
     3181 cagcaggcgg aagcggagct gaaactggcg cgggtgacgt attcccgtca gcaacgtctg
     3241 gcacaaacgc aggcggtttc acagcaggat ctcgacaccg ccgcgacgga gatggctgtg
     3301 aaacaggcgc aaattggcac cattgacgcg caaatcaagc gcaatcaggc ttctctcgat
     3361 acggctaaaa ccaatctcga ttacactcgc atcgttgccc cgatggccgg ggaagtcacg
     3421 caaatcacca ctctgcaagg ccagacggtg attgccgcac aacaagcacc gaacattctg
     3481 acgctggcag atatgagcac catgctggta aaagcgcagg tttctgaagc ggatgtaatc
     3541 cacctgaagc cggggcaaaa agcctggttt acggtacttg gcgatccact gacgcgctac
     3601 gaggggcaaa tcaaggatgt actaccgacg ccggaaaagg ttaacgacgc tattttctat
     3661 tacgcccgtt ttgaagtccc caaccccaat ggtttgctgc ggctggatat gactgcgcaa
     3721 gtgcatattc agctcaccga tgtgaaaaat gtgctgacga tccctctgtc ggcgttaggc
     3781 gatccggttg gcgataatcg ttataaagtc aaattgttgc gtaatggcga aacacgcgag
     3841 cgtgaagtga cgattggcgc acgtaacgat accgatgttg agattgtcaa agggctggaa
     3901 gcgggcgatg aagtggtgat tggtgaggcc aaaccaggag ctgcacaatg acgcctttgc
     3961 tcgaattaaa ggatattcgt cgcagctatc ctgccggtaa tgagcaggtt gaggtgctga
     4021 agggcatcag cctcgatatt tatgcgggtg agatggtcgc gattgttggc gcttcgggtt
     4081 ccggtaaatc gaccctgatg aatattctcg gctgcctgga taaggccacc agcggcacct
     4141 atcgcgtagc cggtcaggat gttgccacgc tggacgccga tgcgctggcg caactgcgcc
     4201 gcgagcattt cggctttatt ttccagcgtt accatttgct ttcgcattta accgccgagc
     4261 agaacgttga agtacccgcc gtctatgctg gtcttgagcg gaaacagcga ctgcttcgtg
     4321 cccaggagtt gctgcaacgg ctggggctgg aagaccgtac agagtattat ccggcacagc
     4381 tttcgggtgg tcagcaacag cgcgtcagca tcgcgcgggc attgatgaac ggcggtcagg
     4441 taattcttgc cgatgaacca accggcgcac tggacagcca ttctggcgaa gaggtgatgg
     4501 cgatcctgca tcagctgcgc gatcgcgggc atacggtgat tatcgtcacc cacgatccgc
     4561 aggtcgctgc tcaggccgag cgggtgatcg aaattcgcga cggcgaaatt gtgcgcaatc
     4621 ctcccgccat tgaaaaagtg aatgttgctg gcgggacgga gcctgttgtc aatacagtgt
     4681 ctggctggcg gcagtttgtc agcggtttta acgaggcgct gacgatggca tggcgggcgc
     4741 tggcagcgaa taaaatgcgt actttactga ccatgctggg gattattatc ggtattgcgt
     4801 cggtggtttc cattgtcgtg gtgggtgacg ccgccaaaca aatggtgctg gcggatattc
     4861 gttctattgg tacgaatact attgatgtct atcccgggaa agattttggc gatgacgatc
     4921 cgcaatatca gcaggcgctg aagtacgacg acttaatcgc catccaaaaa caaccgtggg
     4981 tcgcctcagc cacacctgcc gtctcgcaaa acctgcgcct gcgttataac aatgttgatg
     5041 ttgctgccag tgccaatggc gtgagcggcg attattttaa tgtctatggc atgaccttca
     5101 gtgaaggaaa cacctttaat caggagcagc tgaacggtcg tgcgcaggtc gtggttctcg
     5161 acagtaatac tcgccgccag cttttccccc ataaagcaga tgtggttggc gaggtgattc
     5221 tggtcggcaa tatgcccgcc agagtcattg gtgtggcgga agaaaaacag tcgatgtttg
     5281 gtagcagtaa agtgctgcgt gtctggctac cttacagcac gatgtccggg cgagttatgg
     5341 gccagtcgtg gcttaactcc attactgtca gggtgaaaga aggatttgac agcgccgagg
     5401 cggaacagca actcacgcgt ttactttcac tgcgccacgg taagaaggat ttctttacct
     5461 ggaacatgga cggcgtcttg aaaactgttg aaaagaccac acgtacttta caactgtttc
     5521 tgacgctggt ggcggtgatt tcgctggtgg tgggtggtat tggtgtaatg aatattatgc
     5581 tggtgtcagt gaccgagcgg acgcgggaaa ttggcattcg catggctgta ggtgcgcgag
     5641 caagcgatgt tttgcaacag ttcctgatcg aagccgtact ggtttgcctg gtcggtggcg
     5701 cgttgggaat aactctgtca ctgttaattg ctttcacctt gcagcttttc ttacccggct
     5761 gggagattgg tttttcaccg ttggcactgc tgctggcgtt tctctgctcg acggccaccg
     5821 ggattttatt tggatggtta cccgcacgaa atgcggcacg actggatcca gtagatgctc
     5881 tggcacgaga gtaatttttg agataaaaat gccagccgat cgggctggca ttttgccttt
     5941 aggatgtaca caatgagaca gaagagctat gcgactgccg cttctacttc gacgggcaca
     6001 ataacactgg cgtgattgcc ttttggcccc tggtggacat caaactgaac ggattgtcca
     6061 gcttttagcg ttctataacc atccatctga atggtggaat aatgagcgaa aatatcttcg
     6121 ccgccgcctt cagggcagat gaaaccaaac cctttggcat tgttgaacca cttaacagta
     6181 cccttttcca tgcttcgaca tccttcgcaa atcttataca agtaagatgg aataaaccgg
     6241 ggtcagagag ggggctgttc aaaacctcgc caactctaga aatacaattt agagaattag
     6301 ggcgagccgt caagcatttg acaggggcca gggggcaggt atgaatcaaa aatttgaagc
     6361 agttaacgct attgacagga atgtgacaga tgtcgctgat gccaacgata gatgatagtt
     6421 atctatcatg tggagtagat tggtcaggca aataagctct tgtcagcggc agggcgttct
     6481 gccgataacc gtaaccgaag atgataactg acaatgggta aaacgaacga ctggctggac
     6541 tttgatcaac tggcggaaga aaaagttcgc gacgcgctaa aaccgccatc tatgtataaa
     6601 gtgatattag tcaatgatga ttacactccg atggagtttg ttattgacgt gttacaaaaa
     6661 ttcttttctt atgatgtaga acgtgcaacg caattgatgc tcgctgttca ctaccagggg
     6721 aaggccattt gcggagtctt taccgccgag gttgcagaaa ccaaagtggc gatggtgaac
     6781 aagtacgcga gggagaatga gcatccattg ctgtgtacgc tagaaaaagc ctgaatgcag
     6841 gcataaaaat tgggggaggt gcctatgctc aatcaagaac tggaactcag tttaaatatg
     6901 gctttcgcca gagcgcgcga gcaccgtcat gagtttatga ccgtcgagca cttgttactg
     6961 gcgctgctca gtaacccatc tgcccgggag gcgctggaag cgtgttctgt ggatttggtt
     7021 gcgctccgtc aggaactgga agcctttatt gaacaaacca cacccgttct gcctgccagt
     7081 gaagaggagc gcgacacaca gccgacgctg agttttcagc gtgtactgca acgtgcggtc
     7141 ttccatgtcc agtcctccgg tcgcaatgag gttaccggtg caaacgttct ggtcgctatc
     7201 tttagcgaac aggagtcgca ggcggcatat ctgttgcgta aacatgaagt cagccgtctc
     7261 gatgtggtga attttatctc tcatggcacg cgtaaagacg agccgacaca gtcttctgat
     7321 cctggcagcc agccaaacag cgaagaacaa gctggtgggg aggaacgtat ggagaatttc
     7381 acgacgaacc tgaatcagct tgcgcgcgtg ggcggaatcg acccactgat tggtcgtgag
     7441 aaggagctgg agcgtgctat tcaggttctc tgccgtcgcc gtaaaaacaa cccgctgctg
     7501 gtgggggaat ctggtgtcgg taaaaccgcg attgcggaag gtcttgcctg gcgaattgtt
     7561 cagggcgatg tgccggaagt gatggctgac tgtacgattt actctctcga tatcggttct
     7621 ctgttagcgg gcacaaaata tcgcggcgac tttgaaaaac gttttaaagc gttgctcaag
     7681 cagctggagc aggacactaa cagcatcctg tttattgatg agatccacac cattatcggt
     7741 gcgggggcag cgtctggtgg tcaggttgat gcggctaacc taatcaaacc gttgctctcc
     7801 agcggtaaaa ttcgtgtaat tggttcgaca acctatcagg agttcagcaa cattttcgag
     7861 aaagaccgtg ctctggcgcg tcgcttccag aaaattgata ttactgaacc gtcaatcgaa
     7921 gaaactgttc aaatcatcaa tggcctgaaa ccgaagtatg aagcgcacca cgacgtgcgt
     7981 tataccgcaa aagcggtgcg tgcggcggta gagctggcgg tgaaatacat caatgatcgt
     8041 catctgccgg ataaagcgat tgatgttatc gacgaagcgg gcgctcgcgc acgcctgatg
     8101 ccggtaagca aacgcaagaa aaccgttaat gtggcggata ttgagtccgt ggtggcccgt
     8161 attgcacgca ttccagagaa gagtgtttct cagagtgacc gcgataccct gaaaaacctc
     8221 ggcgatcgcc tgaaaatgct ggtcttcggt caggataaag ccattgaggc gctgactgaa
     8281 gccattaaga tggcgcgtgc aggtttaggt cacgaacata aaccggtcgg ttcgttcctg
     8341 tttgccggtc ctaccggggt cgggaaaaca gaggtgacgg tacagctttc gaaagcgttg
     8401 ggcattgagc ttctgcgctt tgatatgtcc gagtatatgg aacgccatac cgtcagccgt
     8461 ctgattggtg cgcctccggg atacgttggt tttgatcagg gcggtttgct gaccgatgcg
     8521 gtaatcaagc atccacatgc ggtgctgctg ctggacgaaa tcgagaaagc gcatccggac
     8581 gtgttcaata ttctgttgca ggtgatggac aacggtacgc tgaccgataa caacggacgc
     8641 aaagcggact tccgtaacgt ggtgctggtg atgaccacca acgccggggt acgggaaact
     8701 gagcgcaaat ccattggtct tatccaccag gataacagca ccgatgcgat ggaagagatc
     8761 aagaagatct ttacaccgga attccgtaac cgtctcgaca acattatctg gtttgatcat
     8821 ctgtcaaccg acgtgatcca tcaggtggtg gataaattca tcgtcgagtt gcaggttcag
     8881 ctggatcaga aaggtgtttc tctggaagtg agccaggaag cgcgtaactg gctggccgag
     8941 aaaggttacg accgggcaat gggcgctcgt ccgatggcgc gtgtcatcca ggacaacctg
     9001 aaaaaaccgc tcgccaacga actgctgttt ggttcgctgg tggacggcgg tcaggtcacc
     9061 gtcgcgctgg ataaagagaa aaatgagctg acttacggat tccagagtgc acaaaagcac
     9121 aaggcggaag cagcgcatta atctaattgt taggtaggtt ggtgaagtcc gtaatctcga
     9181 aagaggttac ggactttttg tttatggggt ggaacggtga aaaccctatt tttggaggtg
     9241 aaggtaattt gttgataatt agtgctgctg gaaaaggtaa ggataaaaaa gggtgacagc
     9301 aggaaaatga gatggttttg ctttattaac aacggactaa acgtgtagta tttgagttca
     9361 ctgccgtaca ggcagcttag aaatcgacgg ggtgcggtaa aacctttgcg aacgcgttca
     9421 ctgccgtaca ggcagcttag aaattcacag gtaacatact ccacccgccc accatgttca
     9481 ctgccgtaca gaaagataaa atgcgaaaaa aaagctcgca ctttcgtacg agctcttctt
     9541 taaatatggc ggtgaggggg ggattcgaac ccccgatacg ttgccgtata cacactttcc
     9601 aggcgtgctc cttcagccac tcggacacct caccaaattg ttttgctacc aaacctcatg
     9661 ggtggcaacg gggcgctact atagggagtt ggagtaaaac ggtcaagaag aattttaatg
     9721 ataattattg tttgctcata ctgtaaacaa gttgtgcagt atatctacat cgagacaagt
     9781 tacggactta tacttccaaa gtacttcata catatcacaa aataaaaagg ccggttaaac
     9841 cgacctttta ctcgttcttt ctcttcgccc atcaggcggt aaaacaatca gcgactacgg
     9901 aagacaatgc ggcctttgct caggtcgtac ggggtcagtt caacagtcac tttgtcgccc
     9961 gtcaggatgc ggatgtagtt tttgcgcatt ttaccggaga tgtgtgcagt aaccacgtga
    10021 ccgttttcta actctacgcg gaacatggta ttaggcaacg tttcaagaac ggtaccttgc
    10081 atttcaatat tgtcttcttt ggccatctaa tcctctgggg tatcactacc gtaatttgaa
    10141 ccggcaagat aatgccgaag ttctgtaaat aagtaaagat ttgcgcgcta aatcgcaaca
    10201 aacaggttcg gcacattact ccgaaaacac acggctaagc cgcaccaaaa gcgcaacgta
    10261 taagggagcg gtgagataaa cgatgggcgt tacctgacgc gaaaaattcc ttatcggcag
    10321 cggggtaatg agcataacca actctgcgac cgcaattata acactctggg gagaaatgtg
    10381 ccgaaaacat tcatttttgt ggtgaaaaca agcatcgtgg cacccagaaa ttattcggca
    10441 atcgtccgag gcgcatttga ttgagataat tcagataatc ccgacgggga atttcgcagg
    10501 caccaagcga tgctgtgtga tcgttaagga cctggcagtc gataagctta ccgccatgac
    10561 tgataaattc ctcacagaat accagaagcg ccgttttaga cgcattttcc atccggctga
    10621 acatggactc gccacaaaat agcgttccct gggccacgcc gtacataccg ccgacaagct
    10681 catcttcatg ccagacttca atggagtggg catgaccgag ttcgtgaagg cgatggtagg
    10741 cttcgaccac gccacgcgtg atccaggttc cttcttcgcg atcgctggca caaccttcaa
    10801 tgacctgacc aaaagcgtaa ttcatcgtga cacgataggg cgagcgttta tgaaatcgct
    10861 tcatactacg gctgatatgc agtgattcag gccaaagcac cgcgcgagga tcgggcgacc
    10921 accagaggat gggggcgcct ggagaaaacc acggaaaaat accacgctgg taagccatta
    10981 acaggcgcgc agggctaaga tcgcccccca gagccagcag gccgttaggc tcacgtaatg
    11041 cgccttccgg ggaagggaag gctattgaat ggcgagaaag ctgaaccagg cgcatgaccg
    11101 caaaactcca cgcaagtcgg atcgttcaat aatagcttac aaaccctgct taaactggta
    11161 ataacgcccc tgtctggcaa gcagttctgc gtgagtacct tgctcaataa tttgcccgtt
    11221 gtccatcact attatttgtt ggaaacgaga gagtccgcga agtcgatggg tgaccattaa
    11281 caccgttttc tcacgcatca tttctgcaag caattcaagg atctggcttt cggttgtggc
    11341 atctaagcct tcggtaggtt catccagcaa caccagtggc gcatcatgta acagcgcacg
    11401 ggcgatagcc agacggcgca gttcaccacc ggagagctgg cgtccgcctt cacctaacca
    11461 actgttgaga cctgcgtcct cgagcagctt ctccagacca acgcgacgca agatctccga
    11521 cagagcctca tcactactgc caggcgaggc gagtaacaga ttatcacgca gcgtggcgct
    11581 aaacagatgc actcgctgag gaacaacgct gatggtctgt cgtagagccg cttcattcag
    11641 gctggctatg gggctatcgt taagcaaaat ctcgccctgt tgcgggtccc aagcgcgggt
    11701 cagcagttgt aacagtgttg atttgccgca tccggttcgc ccgagaatcg caatatgttc
    11761 cccggcgttt acctgaagag aaatcccttt aagtgcctgt tgggattgct ccggataagt
    11821 gaactgaaca tcccgtaatg tcagcgaaac gcgatcggca acacgagttt gggtatcagg
    11881 gaaggtgact tccggttttt gatccgttaa atcagtgatc cgaacggcag aggcaatgac
    11941 ttgccccaga tgctgaaatg cacccgttac tggtgccagt gcttcaaacg cggctaacgc
    12001 gcagaagaca aacagggcaa ttaacgcgcc gggttgagca ttgccgccaa cgccgccaga
    12061 cgccatccac agcatcagga tcaccgctaa cgcgccaatg agcagcatta tcgcttgcga
    12121 caatgcggtc agttcagatt gacggcgttg cgcttccagc cattgaattt ctgtattctc
    12181 tagttgcgtg cgataacgat cgctggcacc aaaaatggtc agctcagctt gcccttgcag
    12241 ccaggccgtc agttgttggc gatactgtcc gcgaagatga gtcagatttt gcccggtgct
    12301 ttttcccgca cgataaaaca gcggtggcat caggaaaagc gtcagtaaca taatgccgcc
    12361 cagcgtaaag gcgagggtga aatcaaggaa acttaacccg actgtcacca ccataatcac
    12421 cacaaaagcg cccaccagcg gcgagataac gcgcaggtaa agatgatcga gcgtatcaac
    12481 atccgccacc acgcgattga gcaattcgcc ctgacgatag cgcgccagtc cggcagggga
    12541 gaggggcaac aatttgctga aggtgtaaat gcgcagatgc tgcaacacgc ggaaagtcgc
    12601 gtcgtgactt accagacgtt caaaataacg cccggcagta cgggtgattg cggcaccacg
    12661 cacgcccgca gcgggtagca tatagttgaa gctgtacagt ccggcacccc ccgcaaccgc
    12721 tgaggccgag aggaaccagc cggaaagtgt caacagaccg atactggcga gcagcgtcac
    12781 aattgccagc acaataccaa gacttaacat ccatttatga cgtttataca gtgccagata
    12841 gggtagcaaa gcgcgcattt aaatctcctc ctgacgatgg gccagtaatg tggcgaatgg
    12901 gccaccagcc acacttaatt ccgcgtaacg tccttgctca ataatctgac catcctgcat
    12961 tacccaaatg acatcccagt cagcaagatc ttctaactgg tgggtgacca ttaacgttgt
    13021 ctggcgcaga gaggcggcat tcagcgcctc cattacgcgc tgttcactgt gagcatcaag
    13081 gctggcagcg ggttcatcca acagtaatag cgaacaggga tttagtaacg cacgggccac
    13141 cgccacgcgc tgcgcctgcc ccacggaaag gcgggcagcc tggtcgccaa caggcgtatc
    13201 aacgccttgt ggcaggagcg gtagaaactc gctgacccag gcgttatcca gcgctgcttg
    13261 taattcatgt tcgctggcat caggtcgcgc cagtagtacg ttatcccgta atgttgctgc
    13321 cggtaattgt ggattttgcc caacccagga gagatgttta cgccatgatt ctggtgataa
    13381 atcgcgtaat tctatcccgt tgattcgtag cgatccctga tatgagagaa aaccagaaag
    13441 cgcgttcaac agtgaacttt tacctgaacc gctgcgacca accaacacgg cacgttggcc
    13501 tgctggcaaa gtaaagttca gcggtccggc cagcgtttta ccttccggcg acgtgataaa
    13561 cagatcctcg gcctcaatgg tcaccggatc ggtcgatgct aattccacct cgccacgctg
    13621 cggatgggcg agcggggttt ccataaacgt tttcagactg tcagctgcac caacagcctg
    13681 ggctttagca tgataaaacg taccgagatc gcgtaatggc tggaaaaact ctggcgcaag
    13741 gatcagggcc agaaaacccg cagccagcgt cacgccagta tcgtagtgac caaaatccag
    13801 ctcgccgaga taggaaaaac caaagtagac cgccaccaga gcaattgaca gcgaggtaaa
    13861 aaattcgaga atgccggagg ataaaaatgc cagccgtagc acttccattg tccgttggcg
    13921 gaaatcttcc gaagcagaac gaatactttc aatttcagct tcaccacgac caaaaatacg
    13981 caatgtttcc atgccgcgca ggcgatcgag gaaatgccca cttaagcgag caagagcgag
    14041 aaagttacgt cggttagcat cggcagcccc cattccaacc agcgccataa acaacggaat
    14101 taacggtgca gtgcccagca gaatgagcgc cgcagcccag tttgagggga agattgccac
    14161 cacaatcagc aacggcaccg acactgccag cgccatttgc ggcaggtagc gtgcatagta
    14221 atcatgcata tcgtcaattt gctcgagtac cagcgtcgcc cagctccccg caggtttacc
    14281 ctgaatccac gctggccctg cttgttgcag acggtcgaga acctgacggc ggatggcaaa
    14341 gcggatatgc tgcccggcgt gataacccac ccgttcgcgt aaccagaccg cccatgcgcg
    14401 cagtacaaag gtcagaacca gtaacgtaaa gggaagcagc agggcttcac ggggaatatt
    14461 ctccataatc atatgttgca gaattcgcgc catgaaccag gcctgggcaa tgatcaatat
    14521 gccgcttaca aagcccagca gacgagaaat attcagccaa cgttgggaga tgacgctttg
    14581 ctgttttaac cagcgggtta actctttttg acgagattta ttcattgcac gcttagcagg
    14641 tgagttatca gaattatttg cagagcaatg ttacaacggg gaaaaaataa aggcgaccca
    14701 tagtcgcaag gtgtcgcctt ctttactttt gttactgatt tgtaaaatta ttttgcgtca
    14761 gctaaaccat cgaggtagcg ttccgcatca agtgctgcca tgcagccagt accggccgaa
    14821 gtaatggcct ggcgataaat gtgatccatc acgtcgcctg cggcaaagac gccaggaatg
    14881 ctggtctggg tggcattacc atgaataccc gactgtactt tgatgtagcc gttttccagt
    14941 tccagctgcc cttcgaaaat cgcagtattc gggctgtgac cgatagcaac aaacagaccg
    15001 gcaacgtcga gtgactcgat gttatcgctg ttttgcgtat cgtgcagacg aacgccagtg
    15061 acacccattt gatcgccggt cacttcttcc agcgtacggt tggtgtgcag aatgatgttg
    15121 ccgttctcca ctttatccat cagacgctta ataaggattt tttccgcgcg gaaaccgtca
    15181 cggcggtgaa tcagatgcac ttccgaagcg atgttagaca gatacagcgc ctcttcaacc
    15241 gcggtattgc cgccgccgat gaccgcaact ttctggttgc gatagaagaa accgtcgcag
    15301 gttgcacaag cagaaacccc acggccttta aaggcttctt cagagggcag gccgagatag
    15361 cgtgcagaag ctccggtggc aataatcagc gcgtcgcaag tgtattcgcc gttatcgcca
    15421 ttcagacgga acggacggtt ttgcagatcc accttgttga tatgatcaaa aatgatctca
    15481 gtttcaaact tggtggcatg ttcgtgcatg cgctccatta ataacggacc ggtcagatcg
    15541 tttggatcac caggccagtt ttccacttcc gtggtggtgg tcagttggcc gcctttttcc
    15601 atgccggtaa tcagcacagg ttgcaggttg gcgcgcgccg cgtagacagc agcggtgtat
    15661 cccgccgggc ctgaacccag gataagcagt ttactgtgtt tggtcgtgcc catgagatcc
    15721 ccatagttgt tggcagacaa tgggcaggat tgtagggaat ttacagacgt aaaaaaagag
    15781 tatgacgatt ttgttaacaa tttgtgcaat cggcagcatc gataagcagg tcaaattctc
    15841 ccgtcattat cacctctgct acttaaattt cccgctttat aagccgatta aatgatgaat
    15901 aaacgcccct gttaatgaat atctggcatg ttgtactaaa aatcgatgtt ttgctttgac
    15961 aatcccctgg tgttttgcga aaacattcga ggaagaaaaa aaacagtatt cttatatgcg
    16021 cataaccatg catgtaaata ccatgtttac cgtgctagtg aaatctacgt atggcgtgga
    16081 cagacgccat tcgtgatgtc gatagctgcc acaaggcaac ggtcttctca ccgtagaccc
    16141 aggcattgcg cgccgtgaat cttcatgatt tcggtctatc gtgacgggta gcgactctga
    16201 acagtgatgt ttcaggggca gacaggagta gggaaggaat acagagagac aataataatg
    16261 gtagatagca agaagcgcct tggcaaagat ctcgaccgta tcgatcgtaa cattcttaat
    16321 gagttgcaaa aggatgggcg tatttctaac gtcgagcttt ctaaacgtgt gggactttcc
    16381 ccaacgccgt gccttgagcg tgtgcgtcgg ctggaaagac aagggtttat tcagggctat
    16441 acggcgctgc ttaaccccca ttatctggat gcatcacttc tggtattcgt tgagattact
    16501 ctgaatcgtg gcgcaccgga tgtgtttgaa caattcaata ccgctgtaca aaaacttgaa
    16561 gaaattcagg agtgtcattt agtatctggt gatttcgact acctgttgaa aacacgcgtg
    16621 ccggatatgt cagcttaccg taagttgctg ggggaaaccc tgctgcgtct gcctggcgtc
    16681 aatgacacac ggacatacgt tgttatggaa gaagtcaagc agagtaatcg tctggttatt
    16741 aagacgcgct aacacggaac aggtgcaaaa tcggcgtatt ttgattacac tcctgttaat
    16801 ccatacagca acagtactgg ggtaacctgg tactgttgtc cgttttagca tcgggcagga
    16861 aaagcctgta acctggagag cctttcttga gccaggaata cactgaagac aaagaagtca
    16921 cattgacaaa gttaagtagc ggccgccgcc ttctggaggc gttgctgatc cttattgtcc
    16981 tgtttgccgt ctggttgatg gctgccttac taagctttaa cccttcggac cccagctggt
    17041 cgcaaacggc ctggcatgaa cctatccata atttaggtgg gatgcccggt gcgtggttgg
    17101 cagatacgct gttctttatt tttggcgtga tggcttacac cattcccgtc attattgtcg
    17161 gcggttgttg gtttgcctgg cgtcatcagt ccagcgacga atacattgat tattttgccg
    17221 tttcgctacg catcattggc gttttggcgc tcatccttac ctcctgtggt ctggcggcaa
    17281 tcaacgctga cgatatctgg tattttgcct ccggtggcgt cattggcagc ttactaagca
    17341 ctacgctaca accactgcta cacagtagcg ggggaactat tgcgctgctc tgcgtttggg
    17401 cagcgggcct gacgttgttc accggttggt catgggtgac cattgctgaa aaactcggcg
    17461 gctggatttt aaacattctc accttcgcca gtaatcgtac ccgtcgcgat gatacctggg
    17521 tcgatgaaga tgagtatgaa gacgacgaag agtatgaaga tgaaaatcac ggcaaacagc
    17581 atgaatcacg ccgtgcccgt attcttcgcg gcgcgctagc gcgtcgtaaa cggttggcgg
    17641 aaaaattcat taatccgatg gggcggcaaa cagacgctgc gttgttctcc ggtaagcgga
    17701 tggatgatga cgaagagatt acctacactg cacgcggtgt ggctgccgac cctgacgacg
    17761 tcctattttc gggcaatcgt gcaacgcagc cagaatatga cgaatacgat ccattattaa
    17821 acggtgcgcc aattaccgaa cctgtcgctg tagcagctgc tgctaccacg gcggcacaaa
    17881 gctgggctgc gccggttgaa cctgtgactc agacgccgcc tgttgcctct gttgatgttc
    17941 caccttcgca acctacagta gcctggcagc ctgtaccggg tccacaaacg ggagagccgg
    18001 ttattgctcc tgcaccggaa ggttacccac agcagccaca atatgcgcag cctgcagtgc
    18061 aatataatga gccgctgcaa caaccagtac agccacagca gccgtattat gcacctgcag
    18121 ctgaacaacc tgtgcaacaa ccttactacg ctcctgcggc ggaacaacct gttcagcaac
    18181 cgtattacgc ccctgcgcca gaacaaccgg tggcaggtaa cgcctggcaa gccgaagagc
    18241 agcaatccac ttttgctcca cagtctacat accagactga gcaaacttat cagcaaccag
    18301 ccgctcagga gccgttgtac caacagccgc aacccgttga acagcagcct gttgtggagc
    18361 ctgaacccgt tgtagaagag acaaaacccg cgcgtccgcc actttactat tttgaagaag
    18421 tggaagagaa gcgagcccgt gaacgtgaac aacttgcggc ctggtatcaa ccgattccag
    18481 aaccggttaa agaaccagaa ccgatcaaat cttcgctgaa agcaccttct gttgcagcag
    18541 tacctccagt agaaaccgct gccgctgttt ctccgctggc atctggcgtg aaaaaagcga
    18601 cactggcgac gggggctgcc gcaaccgttg ccgcgccagt cttcagtctg gcaaatagcg
    18661 gtggaccgcg tccgcaggtc aaagagggga ttggcccgca gttgccacga ccgaaacgta
    18721 tccgcgtgcc aactcgccgt gaattggcgt cttacggtat taagctgccc tcacagcgtg
    18781 cggcggaaga aaaagcccgt gaagcccagc gcaatcagta cgattctggc gatcagtaca
    18841 acgatgatga aatcgatgcg atgcagcagg atgaactggc acgtcagttc gcccagacac
    18901 agcagcaacg ctatggcgaa cagtatcaac atgatgtgcc cgtaaacgca gaagatgcag
    18961 atgctgcggc agaggctgaa ctggctcgtc agtttgcgca aactcaacaa caacgttatt
    19021 ccggcgaaca accggctggg gcgaatccgt tctcgctgga tgattttgaa ttttcgccaa
    19081 tgaaagcgtt gctggatgat ggtccacacg aaccgttgtt tacgccaatt gttgaacctg
    19141 tacagcagcc gcaacaaccg gttgcaccgc agcagcagta tcagcagcca caacagccgg
    19201 ttgcgccaca gcagcaatat cagcagccgc aacagccggt tgcgccgcag ccacaatatc
    19261 agcagccgca acaaccggtt gcgccacagc agcaatatca gcagccgcaa caaccagttg
    19321 cgccgcagcc gcagtatcaa cagccgcaac aaccggttgc gccgcagcag cagtatcagc
    19381 agccacaaca gccagttgcg ccacaaccgc aggataccct gcttcatccg ctgttgatgc
    19441 gtaatggcga cagccgtccg ttgcataaac cgacgacgcc gctgccttct ctggatttgc
    19501 tgacaccgcc gccgagcgaa gtggagccgg tagatacctt tgcgcttgaa caaatggctc
    19561 gcctggtgga agcgcgtctg gctgatttcc gtattaaagc cgatgttgtc aattactctc
    19621 cggggccggt tatcactcgc tttgaattga acctggcacc gggcgtaaaa gcggcgcgca
    19681 tttctaactt gtcacgggac cttgcccgtt cactttcgac ggtggcggtg cgtgtcgttg
    19741 aagttattcc tggcaaaccc tatgtaggtc tggagttacc gaataaaaaa cgacaaaccg
    19801 tttatctgcg cgaagttttg gataacgcca aattccgcga taatccgtcg ccattaaccg
    19861 tggtgctggg taaagatatc gccggtgagc cggtggttgc cgatctggcg aaaatgccgc
    19921 acttgttggt tgcggggact accggttccg gtaaatctgt cggtgtgaac gcgatgatcc
    19981 tgagcatgct ttataaagca cagccagaag atgtgcgttt catcatgatc gacccgaaaa
    20041 tgctggagct ttcggtttat gaaggcattc cgcatctgtt aacggaagtc gttactgata
    20101 tgaaagatgc cgccaacgcg ctgcgctggt gtgttaacga gatggagcgt cgttataaac
    20161 tgatgtctgc gctgggtgtg cgtaatctgg cgggttataa cgaaaaaatt gctgaagccg
    20221 atcgcatgat gcgtccgatt ccagacccgt actggaagcc gggtgacagt atggatgccc
    20281 agcatccggt gctgaaaaaa gaaccataca ttgtggtgtt ggttgacgaa tttgccgacc
    20341 tgatgatgac ggtaggtaaa aaagtggaag agctgatagc acgtctggcg caaaaagccc
    20401 gtgccgcggg tatccacctc gtactggcaa ctcagcgtcc atcggttgat gttattactg
    20461 gtctgattaa agcgaatatt ccgacccgta tcgcctttac cgtatccagt aagattgact
    20521 cacgtaccat tcttgatcag gctggcgcgg aatcactgct ggggatgggg gatatgctct
    20581 actctgggcc gaactccacg ttgccggtac gtgtccatgg tgcttttgtt cgcgatcagg
    20641 aagttcatgc cgtggtgcag gactggaaag cgcgtggtcg cccacagtat gttgatggca
    20701 tcacttccga cagtgaaagc gaaggtggcg cgggcggttt cgatggcgct gaagaactgg
    20761 atccgttgtt cgatcaggcg gtgcagtttg tcactgaaaa acgcaaagcg tcaatttctg
    20821 gcgtacagcg tcagttccgc attggttata accgtgcagc gcgtattatc gaacagatgg
    20881 aagcgcaggg gattgtcagc gaacaggggc acaacggtaa tcgtgaagtg ctggctccac
    20941 cgccgtttga ctaattaatg cattgccgga taaggcgcgg tagcgtcgca tccggcactc
    21001 tatcaactga aaattcagta ttttcttctt tcctcaagct gattattagc ctggaataga
    21061 gagtagaggg aactcccgat cgggagtgac gtaatttgag gaataatgat gaaaaaaatt
    21121 gccatcacct gtgcattact ctcaagctta gtagcaagca gcgtttgggc tgatgccgca
    21181 agcgatctga aaagccgcct ggataaagtc agcagcttcc acgccagctt cacacaaaaa
    21241 gtgactgacg gtagcggcgc ggcggtgcag gaaggtcagg gcgatctgtg ggtgaaacgt
    21301 ccaaacttat tcaactggca tatgacacaa cctgatgaaa gcattctggt ttctgacggt
    21361 aaaacactgt ggttctataa cccgttcgtt gagcaagcta cggcaacttg gctgaaagat
    21421 gccaccggta atacgccgtt tatgctgatt gcccgcaacc agtccagcga ctggcagcag
    21481 tacaatatca aacagaatgg cgatgacttt gtcctgacgc cgaaagccag caatggcaat
    21541 ctgaagcagt tcaccattaa cgtgggacgt gatggcacaa tccatcagtt tagcgcggtg
    21601 gagcaggacg atcagcgcag cagttatcaa ctgaaatccc agcaaaatgg ggctgtggat
    21661 gcagcgaaat ttaccttcac cccgccgcaa ggcgtcacgg tagatgatca acgtaagtag
    21721 aggcacctga gtgagcaatc tgtcgctcga tttttcggat aatacttttc aacctctggc
    21781 cgcgcgtatg cggccagaaa atttagcaca gtatatcggc cagcaacatt tgctggctgc
    21841 ggggaagccg ttgccgcgcg ctatcgaagc cgggcatctg cattctatga tcctctgggg
    21901 gccaccgggt accggcaaaa caaccctcgc tgaagtgatt gcccgctatg cgaacgctga
    21961 tgtggaacgt atttctgccg tcacctctgg cgtgaaagag attcgcgagg cgatcgagcg
    22021 cgcccggcaa aaccgcaatg caggtcgccg cactattctt tttgttgacg aagtgcaccg
    22081 attcaacaaa agccagcagg atgcatttct gccacatatt gaagacggca ccatcacttt
    22141 tattggcgca accactgaaa acccgtcgtt tgagcttaat tcggcactgc tttcccgtgc
    22201 ccgtgtctat ctgttgaaat ccctgggtac agaggatatt gagcaagtac taactcaggc
    22261 gatggaagac aagacccgtg gttatggtgg tcaggatatt gttctgccag atgaaacacg
    22321 acgcgccatt gctgaactgg tgaatggcga cgcgcgccgg gcgttaaata cgctggaaat
    22381 gatggcggat atggccgaag tcaatgatag cggtaagcgg gtcctgaagc ctgaattatt
    22441 gaccgaaatc gccggtgagc gaagcgcccg ctttgataac aaaggcgatc gcttttacga
    22501 tctcatttcc gcactgcata agtcggtacg tggtagcgca cccgatgcgg cgctctactg
    22561 gtatgcgcga attattaccg ctggtggcga tccgttatat gtcgcgcgtc gttgcctggc
    22621 aattgcttcc gaagatgtcg gcaatgccga cccacgggcg atgcaggtgg caattgcggc
    22681 ctgggattgc tttactcgcg ttggcccggc ggaaggtgaa cgcgccattg ctcaggcgat
    22741 tgtttacctg gcctgcgcgc caaaaagcaa cgctgtctac actgcgttta aagctgcgct
    22801 ggcggatgct cgcgaacgcc cggattatga cgtgccggtt catttgcgta atgcgccgac
    22861 gaaattaatg aaggaaatgg gctacgggca ggaatatcgt tacgctcatg atgaagcaaa
    22921 cgcttatgct gccggtgagg tttacttccc gccggaaata gcacaaacac gctattattt
    22981 cccgacaaac aggggccttg aaggcaagat tggcgaaaag ctcgcctggc tggctgaaca
    23041 ggatcaaaat agccccataa aacgctaccg ttaatgttat cgttgcggta atgttgttac
    23101 tgtatccctg tggtcgcagg ctgtggccac atctcccatt taattcgata agcacaggat
    23161 aagcatgctc gatcccaatc tgctgcgtaa tgagccagac gcagtcgctg aaaaactggc
    23221 acgccggggc tttaagctgg atgtagataa gctgggcgct cttgaagagc gtcgtaaagt
    23281 attgcaggtc aaaacggaaa acctgcaagc ggagcgtaac tcccgatcga aatccattgg
    23341 ccaggcgaaa gcgcgcgggg aagatatcga gcctttacgt ctggaagtga acaaactggg
    23401 cgaagagctg gatgcagcaa aagccgagct ggatgcttta caggctgaaa ttcgcgatat
    23461 cgcgctgacc atccctaacc tgcctgcaga tgaagtgccg gtaggtaaag acgaaaatga
    23521 caacgttgaa gtcagccgct ggggtacccc gcgtgagttt gactttgaag ttcgtgacca
    23581 cgtgacgctg ggtgaaatgc actctggcct cgactttgca gctgcagtta agctgactgg
    23641 ttcccgcttt gtggtaatga aagggcagat tgctcgcatg caccgcgcac tgtcgcagtt
    23701 tatgctggat ctgcataccg aacagcatgg ctacagtgag aactatgttc cgtacctggt
    23761 taaccaggac acgctgtacg gtacgggtca gctgccgaaa tttgctggcg atctgttcca
    23821 tactcgtccg ctggaagaag aagcagacac cagtaactat gcgctgatcc caacggcaga
    23881 agttccgctg actaacctgg tacgcggtga aatcatcgat gaagatgatc tgccaattaa
    23941 gatgaccgcc cacaccccat gcttccgttc tgaagccggt tcatatggtc gtgacacccg
    24001 tggtctgatc cgtatgcacc agttcgacaa agttgaaatg gtgcagatcg tgcgcccgga
    24061 agactcaatg gcggcgctgg aagagatgac tggtcatgca gaaaaagtcc tgcagttgct
    24121 gggcctgccg taccgtaaaa tcatcctttg cactggcgac atgggctttg gcgcttgcaa
    24181 aacttacgac ctggaagtat ggatcccggc acagaacacc taccgtgaga tctcttcctg
    24241 ctccaacgtt tgggatttcc aggcacgtcg tatgcaggca cgttgccgca gcaagtcgga
    24301 caagaaaacc cgtctggttc ataccctgaa cggttctggt ctggctgttg gtcgtacgct
    24361 ggttgcagta atggaaaact atcagcaggc tgatggtcgt attgaagtac cagaagttct
    24421 gcgtccgtat atgaacggac tggaatatat tggctaatac ccaatttttc tgaatctaaa
    24481 aagcgcctgc gggcgctttt tttgtctccc tttgataccg aacaataatt actcttcact
    24541 tacacgtaat actactttcg agtgaaaatc tacctatctc tttgattttc aaattattcg
    24601 atgtatacaa gcctatatag cgaactgcta tagaaataat tacacaatac ggtttgttac
    24661 tggaatcaat cgtgagcaag cttgagtgag ccattatgaa aacgaaaatc cctgatgcgg
    24721 tattggctgc tgaggtgagt cgccgtggtt tggtaaaaac gacagcgatc ggcggcctgg
    24781 caatggccag cagcgcatta acattacctt ttagtcggat tgcgcacgct gtcgatagcg
    24841 ccattccaac aaaatcagac gaaaaggtta tctggagcgc ctgtacagtt aactgtggta
    24901 gtcgctgccc gctacgtatg cacgtcgtgg acggtgaaat caaatatgtc gaaacggaca
    24961 ataccggcga tgacaattac gacggcctgc accaggttcg cgcctgcctg cgtgggcgtt
    25021 ccatgcgtcg ccgtgtctac aatccggacc gcctgaaata tccgatgaaa cgagtcgggg
    25081 cgcgcggtga aggcaaattc gagcgcatta gctgggaaga agcctacgac atcatcgcga
    25141 ccaatatgca gcgcctgatc aaagagtacg gcaacgagtc tatctatctg aactatggca
    25201 ccggtacgct gggcggcacc atgacccgct cctggccgcc gggaaatacc ctggtcgcgc
    25261 ggctgatgaa ctgctgcggc ggctatctga accattacgg cgactactcc tccgcgcaaa
    25321 ttgcggaagg tttgaactat acctacggcg gctgggcaga tggcaacagc ccgtcggata
    25381 tcgaaaacag taagctggta gtgctgtttg gtaataaccc tggcgaaacg cgaatgagtg
    25441 gcggtggggt gacttactat cttgaacagg cacgccagaa atctaatgcc cgcatgatca
    25501 tcatcgatcc gcgctatacc gacaccggtg ccgggcgcga agatgagtgg atccctattc
    25561 gtccgggaac agatgccgca ctggttaacg gtctggcgta cgtcatgatc actgaaaacc
    25621 tggtggatca ggcattcctc gataaatatt gcgttggcta cgatgagaaa accctgccag
    25681 ccagtgcgcc gaaaaatggc cactataaag cttatattct gggtgaaggg ccagatggcg
    25741 tggctaaaac accggaatgg gcctcacaaa tcaccggtgt tccggcagac aaaatcatca
    25801 aactggctcg tgaaatcggc agtaccaaac cggcgtttat cagccaggga tggggcccgc
    25861 agcgtcacgc taacggtgaa atcgcaaccc gtgctatctc gatgctggcg attctgaccg
    25921 gtaacgttgg tattaatgga gggaacagcg gcgcgcgtga aggttcatac agcttaccgt
    25981 ttgtccgtat gccgaccttg gaaaacccga tccagaccag catttcgatg tttatgtgga
    26041 ccgatgccat tgaacgtggc ccggaaatga cggcgctgcg tgatggtgta cgcgggaaag
    26101 ataagctgga tgtgccgatc aaaatgatct ggaactatgc cggtaactgc ctggttaacc
    26161 agcattctga aatcaaccgt acccatgaaa tccttcagga tgataagaag tgcgagctga
    26221 ttgtggttat cgactgccac atgacctcat cggcgaaata tgctgacatc ctgctgcctg
    26281 actgcaccgc ttccgaacag atggactttg cgctggatgc atcctgcggg aatatgtctt
    26341 acgtgatttt caacgatcag gtgattaaac cgcgctttga atgtaagacc atctatgaaa
    26401 tgaccagcga actggcaaaa cgtcttggcg ttgagcaaca gtttactgaa ggccgtaccc
    26461 aggaagagtg gatgcggcat ctgtatgccc agtcgcggga agcgattcct gaactgccaa
    26521 cgtttgaaga gttccgcaag caggggatct ttaaaaagcg cgacccacaa gggcatcacg
    26581 ttgcttataa agccttccgt gaagatccgc aggcaaaccc actgactacg ccatcgggca
    26641 aaattgagat ttattcgcag gcgctggctg acattgccgc tacctgggaa ttgccagaag
    26701 gcgatgtgat cgatccactg ccgatctaca cgccgggctt tgaaagttat caggatccgc
    26761 tgaacaaaca gtatccgctg cagcttacgg gtttccacta taaatctcgc gttcactcaa
    26821 cttacggcaa cgttgatgtg ctgaaagcag cttgccgtca ggaaatgtgg atcaacccgc
    26881 ttgatgcgca aaaacgcggt atcaacaacg gcgataaagt caggatcttt aacgatcgtg
    26941 gtgaagttca tattgaggcg aaagtgacgc cacgaatgat gccaggtgtg gtcgcactgg
    27001 gtgaaggtgc ctggtatgac ccggatgcaa aacgtgtcga taagggtggt tgtattaacg
    27061 tactgaccac tcaacgtccg tctcctctcg ctaaggggaa tccgtcacat acaaaccttg
    27121 ttcaggttga aaaggtgtaa ggagtaaccg atgacaaccc agtatggatt ttttattgat
    27181 tccagccgtt gcaccggttg caaaacctgc gagctggctt gtaaagacta caaagatttg
    27241 acgccagaag tcagcttccg ccgcatttat gaatatgctg gcggcgactg gcaggaagat
    27301 aacggtgtct ggcaccagaa cgtgtttgcc tactatctgt cgatttcgtg caaccactgc
    27361 gaagatccgg cttgtactaa agtctgcccg agcggtgcga tgcataaacg tgaagatggt
    27421 tttgttgtgg tcgatgaaga tgtgtgcatt ggctgccgct actgccatat ggcttgcccg
    27481 tatggcgcac cgcaatataa cgaaacgaaa ggccatatga ccaaatgcga tggttgttat
    27541 gaccgtgttg cggagggtaa aaagccgatc tgtgttgaat cttgtccgct gcgggcgctg
    27601 gatttcggcc ctatcgacga gctgcgtaaa aaacatggcg atctggcggc cgttgcgccg
    27661 ttgccgcgag ctcactttac caaaccgaat attgtgatca aacccaatgc caatagccgc
    27721 ccgaccgggg ataccactgg ctatctggca aacccgaagg aggtgtgaga tgggaagtgg
    27781 atggcatgaa tggccgctga tgatcttcac ggtcttcggg caatgtgtgg cgggtggttt
    27841 tatcgttctg gctttggcgc tgctcaaagg cgacctgcga gcagaagccc agcagcgtgt
    27901 tatcgcctgc atgtttggct tatgggtgct gatgggcatt ggctttatcg cttctatgct
    27961 ccatcttggt tcaccgatgc gcgcttttaa ctcgctcaac cgggtagggg cttcagcact
    28021 cagtaacgaa atcgccagcg gttcgatctt ttttgccgta ggcggcatcg gctggttgct
    28081 ggcaatgctg aaaaagctgt caccggcatt gcgtacgctt tggctggtgg tgacgatggt
    28141 tcttggcgtc gtctttgtct ggatgatggt gcgtgtgtat aacagcattg ataccgttcc
    28201 gacctggtac agcatctgga cgccgatggg cttcttcctg acgatgttca tgggcggccc
    28261 gttactgggg tacctgttgc tgagtctggc tggcgtcgat agctgggcga tgcgtctgct
    28321 gccagccatt tccgtactgg cactggtggt gagtggcgtg gtgtcagtga tgcagggcgc
    28381 agagctggcg actattcata gttctgtgca gcaggccgca gcgctggtgc cggactatgg
    28441 tgcgctgatg tcctggcgga tcgtgctttt ggccgttgcc ctgtgcttgt ggattgcacc
    28501 acagctaaaa ggttatcagc ctgcggttcc gctactatcc gtctcattca ttctgcttct
    28561 ggcaggggaa ttaatcggtc gcggcgtatt ctatggtttg catatgaccg tggggatggc
    28621 cgtcgcaagc taatcagtaa caaccggggt ttcggccccg gttctctttt atttctgctt
    28681 cgttaacgtg tcataactgg tcatcaaatt acgataatca ggaatatggt tagagaacaa
    28741 cgtcgccagt ccttcaatat cattacgcca gtcacggtgc agctcgcaag ccacgccgaa
    28801 ccatgtcatc aattgcgcac ctgcttgcga catgcgatcc catgccgaat gccgggtaat
    28861 ttcattaaag gtccctgacg cgtcagtcac aacaaacaca tcaaaccctt cttcaatggc
    28921 tgaaagcgcc gggaatgcca cgcaaacttc ggttaccaca ccggcaataa ttaactgttt
    28981 tttacctgtc gctttgactg cttttacaaa atcttcgtta tcccaggcgt taatattgcc
    29041 cgggcgagca atgtaaggtg catcggggaa ttgtgctttc agttctggaa ctaatgggcc
    29101 gttaggaccg gtttcaaaac tggtggtaag aatagttggt aagttgaaat acttagccag
    29161 gtcacccagc gccagcacgt tatttttaaa cttatcgggt tcgatatccc gtacaaggga
    29221 aagtaaacca gcctggtgat caacaagcaa aacagcagca tcatttttat caagacgaac
    29281 atacggtttg gtcatcctct tctcctttcg agagtcagtg gtacgcctga aatcggtcag
    29341 acgtgatgca ttgctctgaa agcatagacg ggaaatatga gtttgctgtg accatgaaat
    29401 ttttcgactg aacgaagcgt atgaaatttg tgttagatca ataaaaacaa tcagatgaga
    29461 ttaatcatct cttcagtatt agcgttatga atgagtaata taaatcgata ataccgccgt
    29521 tgcgatatgc gcgtattttc acccattgac aatgtttttg gcggtggcat gatgcgcatg
    29581 aaatttgaac ttcctcacgg ttttaattca tgtccacgta tacccggcct gtcatgcttt
    29641 tgctgtctgg cctgcttttg ttgactctgg cgatcgcggt gttaaataca ctcgtgccgc
    29701 tttggctcgc ccaggaacac atgtccacat ggcaggtagg cgttgtcagc tcatcctatt
    29761 ttaccggcaa ccttgtcggt acattgctga cagggtatgt cattaaacgc attggtttta
    29821 accgcagcta ttatctggcc tccttcattt ttgccgctgg ctgtgccggc cttggcctga
    29881 tgattggatt ctggagttgg ttggcgtggc gttttgtcgc gggcgtcggc tgtgccatga
    29941 tttgggtagt cgttgagagc gcgctgatgt gcagtgggac gtcacgtaac cgtgggcgtt
    30001 tgcttgctgc atacatgatg gtttattacg tgggaacgtt tttaggccag ttactggtca
    30061 gcaaggtctc aaccgagctg atgagcgtat tgccgtgggt tacaggtttg acattggcag
    30121 ggatcttacc gctgttgttt acgcgtgtcc tgaatcagca ggctgaaaac catgattcga
    30181 cgtcaattac ggcaatgcta aaactccgtc aggcgcggct tggcgtgaat ggctgcatta
    30241 tctcaggaat cgttctggga tctctatatg gcctgatgcc tctgtacctc aatcacaaag
    30301 gggtgagcaa tgccagtatt ggtttctgga tggcggtact ggtcagtgcg ggtatcctcg
    30361 gacaatggcc gattggacgt ctggcggata agtttggtcg actgctggtt ttgcgtgttc
    30421 aggtctttgt cgtcattctc ggcagtattg cgatgcttag ccaggcggcg atggccccag
    30481 cgttattcat cctcggtgcc gctggcttta cgctatatcc ggtggcgatg gcatgggctt
    30541 gcgagaaagt tgaacatcat caactggtgg cgatgaacca agccttactg ttgagctata
    30601 ctgtgggaag tctgcttggc ccgtcattta ccgctatgct aatgcagaat ttctccgata
    30661 atttattgtt tatcatgatc gccagcgtat cgtttatcta tttgctgatg ctgctgcgca
    30721 acgccggtca tacgccgaaa cccgttgctc acgtgtaaat gaattcaagc agagtgtgaa
    30781 cttactgttt cacactctgc ttttttgttt cttctatctg acttgcttta ttccaaattt
    30841 tattcgttta aaaataaaat gtgtagcagg ttataatttt gcatttcgct atttccgcac
    30901 ttcttatttg ccgcgcataa tccctcgttt tacagatgcc cctataattt tggcgaagga
    30961 tttgtctatg gctgggaatg ttcaggaaaa acagctgcga tggtacaaca ttgcgctgat
    31021 gtcttttatc actgtctggg gttttggcaa cgttgttaat aactacgcca accaggggct
    31081 ggtggttgtt ttttcatggg tgtttatctt tgcgctctat ttcacacctt atgcgctaat
    31141 tgttggtcag ttaggctcga ccttcaaaga tgggaagggc ggggtcagta cctggattaa
    31201 acacacgatg ggacccggac tggcttatct cgccgcgtgg acctactggg tggtgcatat
    31261 tccctatctg gcacaaaaac cctaggcaat tctgattgcg ctcggttggg cgatgaaagg
    31321 cgacggttcg ctaatcaaag aatattcagt cgtagcgtta caggggttaa cgctggtgct
    31381 gtttatcttc tttatgtggg ttgcttcacg cggtatgaaa tcgctgaaaa tcgtcggttc
    31441 tgtggcaggg attgctatgt ttgttatgtc gctcctgtat gtggcgatgg cggtaaccgc
    31501 gcctgcaatt actgaagtgc atattgcgac cacaaacatt acctgggaaa cgttcattcc
    31561 tcatatcgac tttacctaca ttaccactat ttcaatgctg gttttcgcgg ttggcggagc
    31621 agagaagatt tctccttacg ttaatcaaac gcgcaaccca ggaaaagaat ttccaaaagg
    31681 gatgttatgc ctggcggtga tggttgcggt ttgtgccatt ctgggctcgc tggcgatggg
    31741 gatgatgttt gattcgcgta atatcccgga tgacttaatg accaacggtc agtattacgc
    31801 ctttcagaag ctgggcgagt attacaacat gggtaatact ttaatggtga tttacgccat
    31861 tgcgaatacc ctgggacaag tagcggcgct ggtattctcg attgatgccc cgcttaaagt
    31921 gctattaggt gatgctgaca gcaaatatat tccagccagt ttatgtcgta ccaacgcttc
    31981 tggtacgccc gttaatggct attttctgac cctggtactg gtggcgattc tgattatgct
    32041 gccgactctc ggcattggtg atatgaacaa tctctataaa tggctgttga accttaattc
    32101 ggtagtgatg ccgctgcgtt atttatgggt atttgttgca tttattgcag tcgttcgcct
    32161 ggcgcagaaa tataaaccag aatatgtctt tattcgtaat aagcctctgg caatgaccgt
    32221 cgggatttgg tgttttgcct ttaccgcctt tgcctgtttg acggggatct tcccgaaaat
    32281 ggaagccttc actgcagagt ggaccttcca gttggcgctg aatgttgcaa cgccgtttgt
    32341 gctggtagga ttaggactga tattcccgct gctggcgcgt aaagcgaata gtaaataatt
    32401 attgtggtcg gtgacgctga ctctggcaga tctgacccgt ttttacataa tcgagaaatg
    32461 cgcgcaagcc gcaggacata tactggcggt ttgggtaata gatctggaag cctggacgct
    32521 ctgtgatcca gtcttccagt acacattcta aacgaccggt atcaaggtac tccttgatct
    32581 gttcatagag cagatacccg ataccagcac ccatcaaaac ggcatccagt tcagcatcaa
    32641 catcatcc
//
LOCUS       DAJVQG010000030        32520 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJVQG010000030 DAJVQG010000000
VERSION     DAJVQG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.030"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(134..1210)
                     /gene="glpQ"
                     /locus_tag="OQM83_001279"
     CDS             complement(134..1210)
                     /gene="glpQ"
                     /locus_tag="OQM83_001279"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCS1519578.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1215..2573)
                     /gene="glpT"
                     /locus_tag="OQM83_001280"
     CDS             complement(1215..2573)
                     /gene="glpT"
                     /locus_tag="OQM83_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCS1519579.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2846..4474
                     /gene="glpA"
                     /locus_tag="OQM83_001281"
     CDS             2846..4474
                     /gene="glpA"
                     /locus_tag="OQM83_001281"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCS1519580.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4464..5723
                     /gene="glpB"
                     /locus_tag="OQM83_001282"
     CDS             4464..5723
                     /gene="glpB"
                     /locus_tag="OQM83_001282"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCS1519581.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5720..6910
                     /gene="glpC"
                     /locus_tag="OQM83_001283"
     CDS             5720..6910
                     /gene="glpC"
                     /locus_tag="OQM83_001283"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCS1519582.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7103..8005
                     /locus_tag="OQM83_001284"
     CDS             7103..8005
                     /locus_tag="OQM83_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCS1519583.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQKGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8046..8849)
                     /gene="yfaU"
                     /locus_tag="OQM83_001285"
     CDS             complement(8046..8849)
                     /gene="yfaU"
                     /locus_tag="OQM83_001285"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HCS1519584.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8867..10156)
                     /locus_tag="OQM83_001286"
     CDS             complement(8867..10156)
                     /locus_tag="OQM83_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1519585.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(10213..11417)
                     /gene="rhmD"
                     /locus_tag="OQM83_001287"
                     /pseudo
     CDS             complement(10213..11417)
                     /gene="rhmD"
                     /locus_tag="OQM83_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
     gene            complement(11432..12214)
                     /locus_tag="OQM83_001288"
     CDS             complement(11432..12214)
                     /locus_tag="OQM83_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCS1519586.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(12434..13636)
                     /locus_tag="OQM83_001289"
     CDS             complement(12434..13636)
                     /locus_tag="OQM83_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCS1519587.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(13736..14278)
                     /locus_tag="OQM83_001290"
     CDS             complement(13736..14278)
                     /locus_tag="OQM83_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1519588.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            14557..14982
                     /gene="nudI"
                     /locus_tag="OQM83_001291"
     CDS             14557..14982
                     /gene="nudI"
                     /locus_tag="OQM83_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCS1519589.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(15021..15623)
                     /locus_tag="OQM83_001292"
     CDS             complement(15021..15623)
                     /locus_tag="OQM83_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCS1519590.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            15931..17070
                     /gene="arnB"
                     /locus_tag="OQM83_001293"
     CDS             15931..17070
                     /gene="arnB"
                     /locus_tag="OQM83_001293"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCS1519591.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTHRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            17074..18042
                     /gene="arnC"
                     /locus_tag="OQM83_001294"
     CDS             17074..18042
                     /gene="arnC"
                     /locus_tag="OQM83_001294"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCS1519592.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            18042..20024
                     /gene="arnA"
                     /locus_tag="OQM83_001295"
     CDS             18042..20024
                     /gene="arnA"
                     /locus_tag="OQM83_001295"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCS1519593.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            20021..20911
                     /locus_tag="OQM83_001296"
     CDS             20021..20911
                     /locus_tag="OQM83_001296"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCS1519594.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            20911..22563
                     /gene="arnT"
                     /locus_tag="OQM83_001297"
     CDS             20911..22563
                     /gene="arnT"
                     /locus_tag="OQM83_001297"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCS1519595.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            22560..22895
                     /gene="arnE"
                     /locus_tag="OQM83_001298"
     CDS             22560..22895
                     /gene="arnE"
                     /locus_tag="OQM83_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCS1519596.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            22895..23281
                     /gene="arnF"
                     /locus_tag="OQM83_001299"
     CDS             22895..23281
                     /gene="arnF"
                     /locus_tag="OQM83_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCS1519597.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(23275..23541)
                     /gene="pmrD"
                     /locus_tag="OQM83_001300"
     CDS             complement(23275..23541)
                     /gene="pmrD"
                     /locus_tag="OQM83_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCS1519598.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(23585..25006)
                     /gene="menE"
                     /locus_tag="OQM83_001301"
     CDS             complement(23585..25006)
                     /gene="menE"
                     /locus_tag="OQM83_001301"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCS1519599.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     DYEIISFTSQFCHSIAVDNRFIC"
     gene            complement(25003..25965)
                     /gene="menC"
                     /locus_tag="OQM83_001302"
     CDS             complement(25003..25965)
                     /gene="menC"
                     /locus_tag="OQM83_001302"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCS1519600.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(25965..26822)
                     /gene="menB"
                     /locus_tag="OQM83_001303"
     CDS             complement(25965..26822)
                     /gene="menB"
                     /locus_tag="OQM83_001303"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCS1519601.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(26837..27595)
                     /gene="menH"
                     /locus_tag="OQM83_001304"
     CDS             complement(26837..27595)
                     /gene="menH"
                     /locus_tag="OQM83_001304"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCS1519602.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(27592..29262)
                     /gene="menD"
                     /locus_tag="OQM83_001305"
     CDS             complement(27592..29262)
                     /gene="menD"
                     /locus_tag="OQM83_001305"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCS1519603.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(29351..30646)
                     /gene="menF"
                     /locus_tag="OQM83_001306"
     CDS             complement(29351..30646)
                     /gene="menF"
                     /locus_tag="OQM83_001306"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCS1519604.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(30725..31030)
                     /gene="elaB"
                     /locus_tag="OQM83_001307"
     CDS             complement(30725..31030)
                     /gene="elaB"
                     /locus_tag="OQM83_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCS1519605.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(31085..31546)
                     /locus_tag="OQM83_001308"
                     /pseudo
     CDS             complement(31085..31546)
                     /locus_tag="OQM83_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            31611..>32520
                     /gene="rbn"
                     /locus_tag="OQM83_001309"
     CDS             31611..>32520
                     /gene="rbn"
                     /locus_tag="OQM83_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCS1519606.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVF"
BASE COUNT         7893 a         8803 c         8138 g         7686 t
ORIGIN      
        1 cgttgttggc taaagaaaag ccggtagaag gcagtccact gtttttacta caaatgtgca
       61 tggaacaatt agtggcgatt ggaaaaattt gatatgagac aggatggcgc atgaacgcca
      121 tcctgcagta gcattactct ttattaagga attttactgc cttatcaggg aaatcagtaa
      181 acagcccatt tacacccgct ttgttataca gagcatcata taactgattc acatcagtag
      241 tgtattcagg cagtttatcc gaccgcacgg tataaggatg cactaccagt ttattctgct
      301 gagcatcttg caccatgcca gtgagtttga tattacccgg ctgcgatgtt tcctcaatca
      361 acatatggta atccggcccg ataccgtcgg catattccgc gacttgcttc atggcacctg
      421 gcttaaacat ccagtcatag ctgtaattaa cccagcttcc gtccggctgt ttctgctgcg
      481 tttcattcca gtcggtatag gcaatcagct gtaccagatt gagatccatg cccattttag
      541 gttccagctc attcttaata cgcttcagct catcagcatc aaaacattgc aaataaactt
      601 tatcgtcctt accggtgtaa ccatatttct tcagcacttc cagcgttttt gccgcaatat
      661 ccttcccttc ctgatgatgg aaccacggcg ctttgatttc tggatagata ccgatatttt
      721 tcccggtaga gtgatttaac ccctgaacaa attcaatctc ttcttcaaag gtgtgccccc
      781 ggaagtcgga cttacccatt gggaaacgcc ccggataagt ctgtactttt ttaccgtttt
      841 caatatcgaa accttcggta aatttcagcg acttaatttc atccagcgtg aaatctatcg
      901 cgtagtaacg accgtctttg cgcgcccgat ccgggaaacg atcggcaaca tcagtaacac
      961 gatcgagata atggtcatgc agaacaacca gatggtcgtc tttggtcatc accaaatcct
     1021 gttccagata atccgctccc tgcgcatacg ccatcgcttt tgctggcagc gtatgctccg
     1081 gcaaatatcc actggcaccg cgatgggcga tgactatttt ttcgttgctg tccgccgcca
     1141 ttgcactgct tcccatgact atagtgctca tcatgatcgc catgctaagg tttttcagct
     1201 tcaatttcat gccattagcc tccgttgcgt ttttgcagta attgttcatg gcgacgtttt
     1261 tcgccaatca tcacaacaat caacaagata accgccagaa tgctgccgcc aatcattacc
     1321 ataaagccgc catcccagcc gaagaagtcc acggtgtagc caacaatcgc gctcgccgcc
     1381 accgaaccgc ccaggtaacc aaacagcccg gtaaagcccg ctgccgtacc tgccgctttt
     1441 ttcggtgcca gttccagcgc atgcagaccg atcagcatca caggaccgta gatcaggaag
     1501 ccgataacaa tcatacaaat catatcgacg gttgggttac ctgccgggtt catccagtaa
     1561 acgatagtcg cgatggtcac cagtgtcata aagaaaacgc cggttgcccc acggttgcca
     1621 cggaagactt tatccgacat ccagccgcac agcagagtgc ccggaatacc tgcatattca
     1681 taaaggaagt aggcccagga ggatttatcc agcgcgaaat gcttaacctc tttcagataa
     1741 gtcggtgacc agtcgaggat gccgtaacgc agcagataaa cgaacacgtt ggcgatggcg
     1801 atataccaca gcagtttgtt cggcagtacg tactgcatga agatttgctt cgccgtcagc
     1861 tcctgttccg ctttttcgtt atagtcgtcc ggataatcat ttttgtactc ttcgatcggc
     1921 ggcaagccac aggattgcgg ggtatcgcgc atcatcgcaa aggcgaataa tgccaccaga
     1981 atggcgcaga aagcaggcat atagagcgcc gcatgccagt cattgaacca ggccatcccc
     2041 agcaggaaca gcagcggcgg aataccacca ccgacgttgt gcgcacagtt ccacactgac
     2101 acaatgccgc cacgttcttt ctgcgaccac cagtgcacca tagtacgacc acacggcggc
     2161 caccccatcc cctggaacca accgcagagg aacaacagta caaacatcac cgcaatgctc
     2221 gacgtcgccc atggcacaaa gcccataaac aacatcactg ccgccgccag aatcaaacct
     2281 gcgggcagga aaacgcgcgg attcgagcga tccgataccg aacccatgat gaatttcgaa
     2341 aatccataag caatcgagat ccccgaaagg gcaaaaccta aatcaccgcg tgagaatccc
     2401 tgctcaacca gataaggcat agcaagcgca aagttcttac gaaccaaata gtaagccgca
     2461 tagccaaaga atatccccag gaaaatttgc cagcgcaatc gacgataagt cggatcgatc
     2521 tccgcggcag gtaagcgcgc tttgtgtggc gctggtttaa aaatactcaa cattgatagc
     2581 ctccgtggcc cgtggtctta tttatgatta acagcctgat tcagtgagag aacctgccgt
     2641 ttcttgagtt gccgcgatgt taagaaaaca ttcataaatt aaatgtgaat tgccgcacac
     2701 attattaaat aagatttaca aaatgttcaa aatgacgcat gaaatcacgc ttcactttcg
     2761 aatcatgagc gaatatgcgc gaaatcaaac aattcatgtt tttactatgg ctaaatggta
     2821 aaaaacgaac ttcagaggga taacaatgaa aactcgcgac tcgcaatcaa gtgacgtgat
     2881 tatcattggc ggcggcgcaa cgggagccgg gattgcccgc gactgtgccc tgcgcgggct
     2941 gcgcgtgatt ttggttgagc gccacgacat cgcaaccggt gccaccgggc gtaaccacgg
     3001 cctgctacac agcggtgcgc gctatgcggt aaccgatgcg gaatcggccc gcgaatgcat
     3061 tagtgaaaac cagatcctga aacgcattgc acgtcactgc gttgaaccaa ccaacggcct
     3121 gtttattacc ctgccggaag atgacctctc cttccaggcc acttttattc gcgcctgcga
     3181 agaagcaggg atcagcgcag aagctataga cccgcagcaa gcgcgcatta tcgaacctgc
     3241 cgttaacccg gcattgattg gcgcggtgaa agttccggat ggcaccgttg atccatttcg
     3301 tctgaccgca gcaaacatgc tggatgccaa agaacacggt gccgttatcc ttaccgctca
     3361 tgaagtcacg ggactgattc gtgaaggcgc gacggtgtgc ggtgttcgtg tacgtaacca
     3421 tctcaccggc gaaactcagg cccttcatgc gcctgtcgtg gttaatgccg ccgggatctg
     3481 ggggcaacac attgccgaat atgccgatct gcgcattcgc atgttcccgg cgaaaggatc
     3541 gctgctgatc atggatcacc gcattaacca gcatgtgatc aaccgctgcc gtaaaccttc
     3601 cgacgccgat attctggtgc ctggcgatac catttcgctg attggcacca cctctttacg
     3661 tattgattac aacgagattg acgataatcg agtgacggca gaagaggttg atattctgct
     3721 gcgtgaaggg gaaaaactgg cccccgtgat ggcgaaaacg cgcattttgc gggcctattc
     3781 tggcgtgcgc ccgctggttg ccagcgatga cgacccgagc ggacgtaacg tcagccgtgg
     3841 catcgtgctg ctcgaccatg ctgaacgcga tggtctggac ggatttatca ccatcaccgg
     3901 tggcaaactg atgacctatc ggctgatggc tgaatgggct accgacgccg tgtgccgcaa
     3961 actgggcaac acgcgcccct gtacgaccgc cgatctggca cttcctggtt cacaggaacc
     4021 cgctgaagtt accttgcgta aagtcatctc cctgcctgcc ccgctgcgcg gttctgcggt
     4081 ttatcgtcat ggcgatcgca cgcctgcctg gctgagcgaa ggtcgtctgc accgtagcct
     4141 ggtatgtgag tgcgaagcgg taacagcggg tgaagtgcag tacgcggtag aaaatttaaa
     4201 cgttaatagc ctgctggatt tacgccgtcg tacccgtgtg gggatgggta cctgccaggg
     4261 cgaactctgc gcctgccgcg ctgccggact gctgcaacgt tttaacgtca cgacgtccgc
     4321 gcaatctatc gagcaacttt ccaccttcct taacgaacgc tggaaaggcg tgcaacccat
     4381 cgcctgggga gatgcactgc gcgaaagcga atttacccgc tgggtttatc agggactgtg
     4441 tggtctggag aaggagcaga aagatgcgct ttgatactgt cattatgggc ggcggcctcg
     4501 ccggattact ctgtggcctg caactgcaaa aacacggcct gcgctgtgcc attgtcacgc
     4561 gtggtcaaag cgcgctgcat ttctcatccg gttcgctgga tttgctgagc catctgcctg
     4621 atggtcaacc ggtgacagac attcacagtg gactggaatc tttgcgtcag caggcaccag
     4681 cccatcctta cagtcttctc gaaccacaac gcgtgctcga tctcgcttgc caggcgcagg
     4741 cattgatcgc tgaaagcggt gcgcaattgc agggcagcgt ggaactggcc catcagcgcg
     4801 ttaccccgct cggcactttg cgtgcaacct ggttaagttc gccagaagtt cccgtctggc
     4861 cgctacccgc gaagaagata tgtgtagtgg ggataagcgg gctgatggat ttccaggcgc
     4921 atcttgcggc ggcttcgttg cgcgaactgg atctttcggt tgaaaccgca gaaatagagc
     4981 tgccggaact ggatgtcctg cgcaataacg ccaccgaatt tcgcgcggtg aatatcgccc
     5041 gtttccttga taatgaagaa aactggccgc tgttacttga tgcgcttatt cctgtcgcca
     5101 atacctgcga aatgatcctg atgcccgcct gcttcggcct ggccgatgac aaactgtggc
     5161 gttggttgaa tgaaaaacta ccttgttcac tgatgctttt gccaacgctg ccgccttccg
     5221 tgctgggcat tcgtctgcaa aaccagttac agcgccagtt tgtgcgccag ggtggcgtgt
     5281 ggatgccggg cgatgaagtg aaaaaagtga cctgtaaaaa tggcgtagtg aatgaaatct
     5341 ggacccgcaa tcacgccgat attccgctac gtccacgttt cgcggttctc gccagcggca
     5401 gtttctttag tggcggactg gtagcggaac gtaacggcat tcgcgagccg attctcggcc
     5461 ttgatgtgct acaaaccgcc acgcggggtg aatggtataa gggcgatttt tttgcgccgc
     5521 aaccgtggca gcagttcggt gtaaccactg atgagacgct acgcccgtcg caggcagggc
     5581 aaaccattga gaacctgttt gccatcggtt cggtgctggg cggatttgat cccatcgccc
     5641 aaggatgcgg cggcggtgtt tgtgccgtca gtgctttaca tgccgctcaa cagattgccc
     5701 aacgcgcagg aggccaacaa tgaacgacac cagcttcgaa aactgcatta agtgcaccgt
     5761 ctgcaccacc gcctgcccgg tgagccgggt gaatcccggt tatccagggc caaaacaagc
     5821 cgggccggat ggcgagcgtc tgcgtttgaa agatggcgca ctgtatgacg aggcgctgaa
     5881 atattgcatc aactgcaaac gttgtgaagt cgcctgcccg tccgatgtga agattggcga
     5941 tattatccag cgcgcgcggg caaaatatga caccacgcgc ccgtcgctgc gtaattttgt
     6001 gttgagtcat accgacctga tgggtagcgt ttccacgccg ttcgcaccaa tcgtcaacac
     6061 cgctacctcg ctgaaaccgg tgcggcagct gcttgatgcg gcgttaaaaa tcgatcatcg
     6121 tcgcacgcta ccgaaatact ccttcggcac gttccgtcgc tggtatcgca gcatagcggc
     6181 tcagcaagca caatataaag accaggtcgc tttctttcac ggctgcttcg ttaactacaa
     6241 ccatccgcag ttaggtaaag atttaattaa agtgctcaac gcaatgggta tcggtgtaca
     6301 actgctcagc aaagaaaaat gctgcggcgt accgctaatc gccaacggct ttaccgataa
     6361 agcacgcaaa caggcaatta cgaatgtaga gtcgatccgc gaagctgtgg gagtaaaagg
     6421 cattccggtg attgccacct cctcaacctg tacatttgcc ctgcgcgacg aatacccgga
     6481 agtgctgaat gtcgacaaca aaggcttgcg cgatcatatc gaactggcaa cccgctggct
     6541 gtggcgcaag ctggacgaag gcaaaacgtt accgctgaaa ccgctgccgc tgaaagtggt
     6601 ttatcacact ccgtgccata tggaaaaaat gggctggacg ctctacaccc tggagctgtt
     6661 gcgtaaaatc ccggggcttg agttaacggt gctggattcc cagtgctgcg gtattgcggg
     6721 tacttacggt ttcaaaaaag agaactaccc cacctcacaa gccatcggcg caccactgtt
     6781 ccgccagata gaagaaagcg gcgcagatct ggtggtcacc gactgcgaaa cctgtaaatg
     6841 gcagattgag atgtccacaa gtcttcgctg cgaacatccg attacgctac tggcccaggc
     6901 gctggcttaa actcctttct gatgcccggt aagcatgtgg ttaccgggca tttttgcgta
     6961 cacgattccg tgcccaatgt atgcgttgca acgcagtgaa aattcctctg aaaacgtctc
     7021 gcaaaggctg aaactggcag atgtcaaagg cctgggataa ccgtaatgtc gcgtcatcat
     7081 aaatatcagg tgacggacaa ccatgaccga atcaacaacc tcctccccgc atgatgcggt
     7141 atttaaaacc tttatgttca cacccgaaac cgcacgggat tttctcgaaa tacatttgcc
     7201 agaaccactg cgcaagcttt gcaacctgca aaccttacgc ctggaaccca ctagttttat
     7261 tgaaaaaagt ttacgcgctt actactcgga tgttttgtgg tccgtggaaa ccagcgacgg
     7321 tgacggctat atctactgcg tgattgaaca tcaaagctct gcagaaaaga atatggcttt
     7381 tcggctaatg cgctatgcca ctgccgccat gcagcgtcac ctggataaag gctatgacag
     7441 agttccgctg gtggtgccat tgctgtttta tcatggcgaa acatcgccct acccgtactc
     7501 acttaactgg ctggatgagt ttgacgatcc gcaacttgcc cggcagttgt acaccgaagc
     7561 ttttccgttg gtggatatca ccatcgtacc tgacgatgag atcatgcaac atcggcgtat
     7621 agctctgctt gaactgattc aaaagcatat tcgcgaccgc gatttaatcg gtatggtcga
     7681 caggatcacc acgcttttgg ttaaaggctt cactaatgac agccagctac aaacactgtt
     7741 taattatctg ctgcaatgcg gcgatacctc ccgtttcacc cgttttattg aggagatttc
     7801 cgaacgttca ccactacaaa aggagagatt gatgactatt gctgagcggc tacggcagga
     7861 aggacaccaa attggctggc agaaaggtat gcatgaacaa gccattaaaa ttgctttgcg
     7921 catgctggaa cagggcattg atcgtgacca ggtgctcgcg gccacccagc taagcgaagc
     7981 cgatctggca gcgaataacc actaattaac acaggcccac agccgatccc catgggcctt
     8041 tgatatcaat aactaccttt tatgcgtggc ccatttttgc ctgatttaaa catcgccagt
     8101 cgttgatcca gggcatcgct gtagagcatc gtgtcaacgc caacagcgac aaagttcgct
     8161 ccccacgcca ggcattgctg cgccatatca ggagccacag ccagaaaacc agccgcttta
     8221 cccgcagcac ggatccgccg aatgctggtt tcaataattc gctgcacttc cgggtgcccg
     8281 gcgttatccg ggtagcccaa cgacgcagaa agatccgctg gtccaataaa cacgccatca
     8341 atcccttcga cgtcgaggat ctcgtccagg ttatccagtg ccgttttact ttccacctgc
     8401 actaacagac aaagcgaatc gttgacttgc gccatgtaat tctcaatgcg tccccagcgc
     8461 gcagcccgtg ccacactggc cccaacacca cgctcaccgt agggaggata gcgcgtggca
     8521 gacgccacct gacgagcctg atcggcagta tcgaccatcg ggatcaatag agtttgcgcg
     8581 ccaatatcca gaacttgttt aatcagcgat ttactgcctt ccaccggacg gatcacgggt
     8641 tggctggcat agggcgctac cgcctgtagc tggtgataaa gatcctgaat ggtgtttggc
     8701 gcgtgctccc cgtcaatcag caaccagtca taaccggaag tagcggcaat ttctgccata
     8761 taagaagtag tcgagcttaa ccacagacca atttgcactt cgcccttgcg taaacgttct
     8821 ttaaagggat tggttaataa tgcgttcatc gtgttcctta acctatttaa tgatgtgcca
     8881 cgtcggtctg cgcaacagta cgatttacac gcagagtgaa aataatcagc gaaccgatga
     8941 ccgccaccgc tgccagtgtc agtaatcctg ccgcatcgct ggcaaataac gtttctgctt
     9001 tcacgcgcag gatcggggca ataaacccgc ccactgcacc aaacaggttt acaaaaccaa
     9061 tccccgcagc cagcgccgta ccggaaagca gttgtgtcgg catcgtccag aacaccggct
     9121 gcacggcaat aaacccgata gctgcaacac acagcgctac gatcgccatt actggagaaa
     9181 gcagcccgga cagaccaata ccaatgcccg ccgccagtaa tgtcagcgct gcgacattac
     9241 gccgttcgcc ggttttatcg gaatagcgcg gaataagcca ggtcccaaac aaggccgcaa
     9301 cccacggaat ggcggtgacc accgacgctg taaagcccac ttttgttccc agcaatgccg
     9361 caacctgggt cggcaggaag aaaattaatc cgtaaaccgc cacctgaatg gtcaggtaga
     9421 taatcgctag ttgccagact cggccattac gcagcgcatc gctcagccga gaagtcactt
     9481 tctgttgttc ttcacttgcc agttgattga taagcaacgt tttttcttgt ttactcagaa
     9541 aacgtgcctg ctccggtgtg tcatcaagcc aaaagaatgt gaatacccca gcaccgactg
     9601 ccaacaatcc ttcaatcaca aacatccaga accagccggg atgccccata aatccatgca
     9661 tctccaacag cgcgccggaa agcggtgatc ccagtgttaa cgccagcggt gcgcccatat
     9721 agaacagccc cataatgctg gcgcgattac gctgcggaaa ccattgcgag gtgagataaa
     9781 tcataccagg gaaaaatccg gcctccgcag caccaagcag agtgcgaact atcagaaatt
     9841 tcgcttcagt atcggcccat gccatggctg ccgaaagaaa tccccacagc agtgttgtcg
     9901 taccaatcca ggttctggcc cccagtttgc gcatcaaaag attcgccgga acacccagaa
     9961 acgcatatac cacaaagaaa atgcctgctc ccagcgcata agcttcatta ctcaacccgg
    10021 tatcaatctg gtaggtctgt ttggcaaaac cgatattcga acggtcgaga aacgccagca
    10081 catacagcgc caacataaac ggaattaaac gcacacggtt tttcttcacc acggcgtcaa
    10141 gcaaagcggt gctcataaaa agctccttag aatatgtggg cgttaccgtt gtcacagcaa
    10201 cgcccggagt gattagtggc tgtaggggcg tttcaaattg cagtcacgat tgagttcgac
    10261 gccaaaaccg ggtttatcaa gcactgattt atgaatacga ccattcaccg gaaccggctc
    10321 attgagcaga atcgggtcaa actgcggacg catcgttgaa caatccgggc tggtcatcag
    10381 gaattcgctg aatggcgtat tggtgaaggt gatcaccgca tggtgagagt aaacagacga
    10441 accgtgcggc accaccagtt gccccctgga tttggcgatt gcggcaattt ccaccagcgt
    10501 ggttaaacca ccgcaccagc caacatccgg ctgcataatg tcgataccgg tttctgaaag
    10561 cgtacgaaaa gattgcagtg tgccgtggtg ctcaccgctg gtgaccatca ttccgactgg
    10621 cgcgttgcgt ttcagttcgc gataaccttc atactgctgt ggcggcaggc actcttcgat
    10681 ccatttcagg ttatagggcg cgcaagcgtg ggccagtttg gtggcatagt tcacgtcctg
    10741 actcatccag cagtcgagca ttaaccagaa atcctcaccg catttttcac gcatatccgc
    10801 gaccataacg gcatctttgc ggatccccgc atcgccatca tgtggccccc agtgcgtcgg
    10861 cattttgcca ccgataaagc ccatctcttt tgccagatcc ggacgcgcgc ctgtggcgta
    10921 gaactgaatc tcatcacgaa ctgcgccgcc taaaagttta taaaccggaa gcccgaccac
    10981 tttgccgaac agatcccaca gagccagatc gacacaggaa atcgtgttca tcaccaggcc
    11041 accagagccg gagtaataca gggtggcatt gagcatttga tcatggatca gtttgatatc
    11101 actgacacat ttaccctcaa tgaaacggtt aagatgtttt tcgacaataa aacagcccat
    11161 ttcaccggct gtcgaaacag cgaatccggt ctggccgttt tctgcttcga cttcaacaat
    11221 caacgtgcca agaacgttaa tgccaaatga ctggcgtgac tgctcgtaat cgcggtattt
    11281 actcatcggg gtggcaatat gatcgtcaat ccagtgattc gccccctggt cgtgataatc
    11341 accgcgccag cacctttttc tgctgtcgca ccgccagtaa accaggcgcg aacctgtttg
    11401 atttttggta gggtcatgat gttctccatt gttatgaggc ctgtaagtca aagggacttt
    11461 tccatcccaa cagacgtgaa atatccctgg cgcaggcaat ggccttgccc gccagataat
    11521 cacggtattc ttcattgatt tgtaagcggg taccgaccac cgagatcgca gcggtaagct
    11581 cgttattggc gttaaacacc ggcgcagcga cacaacggac atcggggtaa tcttcgccgt
    11641 tgtcatagct ccagccctga cggcgaatac gcgccagttc ttcgtgaagt tgctgtggat
    11701 gagtaatcgt tgtgggtgtc gcctgctccc agaccagccc ttcgataata ctttgctgta
    11761 ccgctgcagg ttgccaggca agcaggcatt tacctattcc ggagcgataa agcgaaaggc
    11821 tttttccttc atgggaacgc acgctgatag tggctgatga ctccactttt aaaatgtaat
    11881 aagcgctgcc gttatcaata atccccaggt gacataacag cccggtggta tccatcagtt
    11941 gcgtcaaacg cggtcgagcc agttcccgga gatccatctt gcttaacgca tgaccagaaa
    12001 gctccaccag cctggtccac aaacaaaaat tttcctggtg atcaaggcta agaaaacgct
    12061 gacgcctgag ttcattaagc agcaaatagg ccgtgctttt agggattccc aatgtgtcaa
    12121 tgatcgtcgc agcactacag gggccaatgc gggcaatcaa attgagaata tcaatagccc
    12181 gtgtcagggc gggaactttg cttgattcca acatactgga ctccagtctt agatactgga
    12241 atcagtctcg ttgctcaggg ggagtaaaat tgtgaagcgc atcaaatccc gctcctcgtc
    12301 cagcggactg gagaaattac gcacaaattt gtgaatcagt gcacggattt tttcttattg
    12361 agtacaacag gttggacaag ttttgccgga gggggaatgt gccggtgcag taaaccggca
    12421 cagagtggcg gaatcagaca gataaggtca tggactcaac aacctcaatc cagccatgct
    12481 cactggcgat atcctggccg tttaaccaac ggcgcagcat attcagtgcc atcattgcgc
    12541 acacttcctg acggatagcc aggctgtagc gtgtagtgct gaaacgcaca cgcagagcga
    12601 aagtgccgtc tggcgtggct agcgcaaagt tgagatgctc gttctcgaaa cccgaaacag
    12661 ccagtgccag cccggcaaaa tggttggccc gccgttctgt aatccagtgc gcagtttgcg
    12721 ccagggtttc ttcctgtgaa ggaaccactt cacacgccag caatggagca cctgcgcgag
    12781 aaagttgcaa agccaataaa ccaccggtga attgctcgct caacgtcagg ctgaactggc
    12841 ggttttgcaa ttcgcgactg atctgcgcgg gcagtccttc ggtgccttcg aaaatcacgc
    12901 tctgtccggc aacacgttta acgtccagcc acagtttttc catcgcctgt tgctcgcttg
    12961 ccggtccggt gagtttcagt tcgatgatcg gcattgagga gcgatagccc attgttacgc
    13021 ccggcggcag ttgtagagtg tccaggcttt gtgccagatc gctttccgaa cgaccaaagg
    13081 tagttaaacg cagacaaacc ggcgactgtg gtaaagaaaa acgctcgcgc aggcgcggca
    13141 ggatttcgtg ctcgaccatc accttaaatt ctgacggtac gccgggagta aagaacatca
    13201 ggcaacgatt aagccgcacg gcaaaaccac aggcggtgcc taccgggtta ttgataaatt
    13261 cagcactggc aggcagctcc gcttgtttac ggttgctcgg tgccattact cgtccacgtt
    13321 cgtggaaata gcgttccatc tctttgagcc aggcttcatg cagcaccagg ccttcacctt
    13381 ttgctgtcgc agcggcgagt gcacttaaat catcactggt cggtcccagc ccgccgttaa
    13441 cgatcagcac atcggcgtgc tgactacgtt cgcgaagaat ggtgactaag tcatcaaggt
    13501 tatcccccac cgtattgcgg cgagataatg gcaacccctg atgaaagaaa aaatcggcca
    13561 gccaggcagc gttagtgtca acgatttgcc cgtgtaacac ttcatccccg gtggataaca
    13621 tttccacttt taacatggtt atctcctgct tttgttatcg aatcactata acgcaaaggg
    13681 agataaggcg tagtaagaaa ctgaatggcg cgacagaatg ccgcgccgga agggatcaga
    13741 aactggcgtt aaccccaaca tacgggccgt cagccacggc gttgtcgcgg ttaccgtctt
    13801 tacccgacag attcaggtag cgataacccg cctcaatact gactggatgc ataatggtgt
    13861 aacgcgcgcc agcattcgct tcctcataac ttttaatacc gctggagagt gaatccggag
    13921 agtagtaata ctcgccaaac aaacggaagc tgttgccaat tttccactgc aaaccacctc
    13981 ctaccgctgc ggcataacct tcatcgccgt aattcgggtt ggtgtaaacg ccttttccgc
    14041 caacggtcgc cattaacggc ccgagaggca gattcaaccc cagccccacg ccagcgacgt
    14101 caccgtcgtc gtcgttatgt gtccagttac cgcttaacgc caggcccgtc gattcagtcc
    14161 caaaaccgac accaatattg gtgtattctt tacccgcctg accgctgatg ctcattgcat
    14221 tgaccgatgc agaaaccaac agcattccgg ccagacctgc tagcgcaatt tttttcatta
    14281 tcccctttcc cacaagcaat taatcaaaat tcccaaagct gcgggattgt actgcctggc
    14341 acagaggatt caatgagcga aaaaagggat ctagtcactt attgtaacta aatacttctt
    14401 aaataattaa acgccgaaaa aataaacaca ctttaccgat acacacaaaa ataataataa
    14461 gtgcagttat catcgcaaaa gagattactg tcactttcac atatttctga gttaccgtat
    14521 tctcatggtt tctttttatt tataaggaat acccctgtgc gacaacggac tattgtatgc
    14581 cctttgattc aaaatgatgg tgcttatttg ctgtgcaaaa tggccgacga tcgcggcgtt
    14641 ttccccggtc aatgggcgct ttcgggtggc ggcgtggagt ctggcgaacg aattgaagag
    14701 gcactacgcc gcgaaattcg cgaagaactg ggagaacagc tgcttttgac agaaatcacg
    14761 ccgtggacct tcagcgatga tattcgcacc aagacgtatg cagatggtcg caaggaagag
    14821 atttatatga tttacctgat ttttgactgc gtttctgcca accgagaagt gaaaataaac
    14881 gaagagtttc aggactacgc gtgggtaaaa cctgaagatc tggtgcatta tgatttaaat
    14941 gttgccaccc gaaaaacgtt acgtttgaaa ggtcttcttt aacaatgatg acagccatca
    15001 ctggctgtca gattgaaaat ttagtggtta acgaattccc catccagata aactttgcct
    15061 ttttccacat gcatgactaa accatccaga taatcaggtt taaatgtcgc atcacgctta
    15121 tttttagcaa tatatgtcag gcaatgatta tgggtgaaaa taacgatatt tttatcaggc
    15181 gctttgcttt gtaagtcctt aattgcacta taaatctcat taccgcactg aagaagtcgt
    15241 ttatctaccg tcagtttttt acccgcggaa aaccaggtcg ccgactgaat ggtccggacg
    15301 gtattactgg aataaagatc gaaatcaggg atgtcagcgc taaaagcgtt gcccagttca
    15361 cgggcatcct gggtaccttt aaccgtaata cctgttttat ctgacaagca ttgattggtt
    15421 gaacgatcgc aacgttcagc atggcgaaac aaaacgacaa ccgggtgctg ctgtgccagt
    15481 ctggccaatg ttttattgtc gatacgtggc aaaccattgc tactccaggc ggcatgagta
    15541 cccagtccgg caattgcagc gagcgccagt aaaatgatga aatatttttt tgacttcaat
    15601 gatgagcggc aaaaagctaa cacgtaaact ccacctatag acaagcgcaa ccagacaatt
    15661 accgtgaaat tgagcgacat ttctggcgat aattagcagt tggtgtaata ttaaaaatcc
    15721 tacgatgtcg gcaaaatgcc tcaaaatttt gccaaatgca aagcctaaat aagaaaaaat
    15781 ataaaaattt caatatttac gtctaatatt agtttcttaa ggttaagtta atattctatc
    15841 cttaaaattt cgctccaaat ggcaaaatat acacaacact ctttatagca aatataagtg
    15901 gacaggtatt caatggcgga aggaaaagca atgtcagaat ttttgccttt ttcgcgacca
    15961 gcaatgggcg tggaggaact cgctgcagtt aaagaggttc tcgaatccgg ttggatcaca
    16021 accggtccga agaatcaggc gcttgagcaa gccttttgcc agttgacggg aaatcagcat
    16081 gccatcgcgg tcagttcagc caccgccgga atgcatatca cgctaatggc gttggaaatt
    16141 ggcaagggcg atgaagtgat tacgccttcc ctgacctggg tttcaaccct caatatgatt
    16201 tccttgttgg gtgcaacgcc ggtaatggtg gatgtcgacc gcgatacgct gatggtcacg
    16261 cctgaagcga tcgagtcagc cattacgcca cgcactaaag ccatcattcc ggtgcattat
    16321 gccggtgcgc cagcagatat tgacgccatt cgcgccattg gcgaacgtta cggcatcgca
    16381 gttatcgaag atgctgccca tgccgtcggt acgtattaca aagggcgaca tattggcgca
    16441 aaaggtaccg ctattttttc atttcatgcc attaaaaata ttacctgtgc ggaaggtggc
    16501 ctgattgtaa ctgataatga aaaccttgcc cgccagctac ggatgctgaa atttcacggt
    16561 ctgggtgtcg atgcctatga cagacaaacc tggggccgtg caccgcaggc tgaagtctta
    16621 acaccgggct ataagtacaa tctgaccgat attaacgccg cgattgccct gacacagtta
    16681 gcaaaattag agcacctcaa cacccatcgg cgcgaaattg cccagcaata tcagcaagca
    16741 ctggcagctc tcccctttca gccattaagc cttcccgcct ggccgcacgt tcacgcctgg
    16801 catctgttta ttattcgtgt cgatgaacaa cgttgtggta tcagtcgcga tgcgttgatg
    16861 gaagcgttaa aagaaagagg cattggtacc gggttacatt tccgcgccgc tcacacacaa
    16921 aaatattatc gcgagcgttt tcccacgctg tcgttaccga ataccgaatg gaatagcgaa
    16981 cgcatctgtt ctttgccgct gttcccggat atgactaccg ccgatgccga ccgtgtcatc
    17041 acagcccttc agcaactcgc aggacaataa gccatgtttg aaatccaccc tgttaagaaa
    17101 gtctcggtgg ttattcccgt ttataacgag caggaaagct taccggaatt aatcaggcgc
    17161 accaccacag cctgtgaatc gttggggaaa gagtatgaga tcctgctgat tgatgacggc
    17221 agtagcgata attccgcgca tatgctggtc gaagcctcac aagcggagaa cagccatatt
    17281 gtgtctattt tgcttaaccg caattacggg caacattcag cgattatggc gggattcagt
    17341 cacgttactg gcgacttaat tattaccctt gatgccgatc tccagaatcc gccagaagaa
    17401 atcccccgcc tggtggcaaa agccgatgaa ggttacgacg tggtagggac tgtacgccag
    17461 aaccgccagg acagctggtt tcgtaaaaca gcttcgaaga tgattaacag gcttattcag
    17521 cgcaccaccg gcaaagcgat gggtgactac ggttgtatgc tgcgcgccta tcgccgtcat
    17581 attgtcgatg cgatgttgca ctgccatgaa cgtagcacct ttatcccgat tctggcgaat
    17641 atcttcgccc gccgtgccat tgaaattcca gtacatcatg ccgagcgtga gtttggtgaa
    17701 tccaaataca gttttatgcg cctgattaat ttgatgtacg acctggtgac ctgccttacc
    17761 acaacgccgc tacgtatgct gagtctgctc ggcagcatta ttgcgattgg aggttttagc
    17821 attgcggtgc tgctggtgat tttacgcctg accttcggac cacaatgggc ggcagaaggc
    17881 gtctttatgc tatttgccgt gctgtttact tttattggcg ctcagtttat cggcatggga
    17941 ttgctcggtg aatatatcgg caggatctac accgatgtcc gcgcccgccc ccgctatttt
    18001 gttcagcaag ttatccgtcc atccagcaag gaaaatgaat aatgaaaacc gtcgtttttg
    18061 cctaccacga tatgggatgc ctcggtattg aagctctgct ggctgccggt tacgaaatta
    18121 gcgccatttt tacccatacc gataatcccg ctgaaaaagc cttttatggt tcggtggctc
    18181 gtctggcggc ggaaagaggc attccagttt atgcgccgga taacgttaat catccgctgt
    18241 gggtggaacg cattgcccaa ctctcaccag aggtgatttt ctctttttat tatcgccatc
    18301 ttatttgcga cgagattttg cagctcgctc ccgcaggtgc atttaatctg cacggttcgc
    18361 tgttaccaaa atatcgtggt cgcgcgccgc tgaactgggt gctggtgaac ggtgaaacgg
    18421 aaacgggcgt tacattgcac cgaatggtaa aacgtgccga tgctggggcc attgtggcgc
    18481 aactgcgcgt tgccattgcg ccagacgata tcgctattac gctgcatcat aaattgtgcc
    18541 atgccgcgcg ccagctactg gaacagacat tacccgccat taaacacggt aatattctgg
    18601 aaattgccca gcgcgaaaac gaagccacct gttttggtca cagaacgcca gatgacagtt
    18661 tccttgaatg gcataaaccg gcatccgtac tgcacaacat ggtacgtgcc gttgccgatc
    18721 cgtggccggg tgccttcagc tatgttggca atcagaaatt caccgtctgg tcgtcgcgtg
    18781 ttcatcctca tgccagcaaa gcacagccgg ggagcgtgat ttctgttgcg ccactgctga
    18841 ttgcctgtgg cgatggcgcg ctggaaatcg tcaccggaca ggcgggcgac ggcattacta
    18901 tgcagggctc gcaattagcg cagatgctgg gcctggtgca aggttcacgc ttgaatagcc
    18961 agcctgcctg caccgcccga cgccgtaccc gggtactcat cctcggggtg aatggcttta
    19021 ttggcaacca tctgacagaa cgcctgctgc gcgaagatca ttatgaagtt tacggtctgg
    19081 atattggcag cgatgcgata agccgttttc tgaatcatcc gcattttcac tttgtcgaag
    19141 gcgatatcag tattcattcc gaatggattg agtatcacgt caaaaaatgt gatgtcgtct
    19201 tgccgctggt ggcgatagcc acgccgattg aatatacccg caacccgctg cgcgtatttg
    19261 aactcgattt tgaagagaat ctgcgcatta tccgctactg cgttaagtac cgtaagcgaa
    19321 tcatcttccc gtcaacttca gaagtttatg ggatgtgtag cgataaatac ttcgatgagg
    19381 accattctaa tttaatcgtc ggcccggtga ataaaccacg ctggatttat tcggtgtcaa
    19441 aacaattact tgatcgggtg atctgggcct atggcgaaaa agagggttta cagttcaccc
    19501 tcttccgccc gtttaactgg atgggaccac gactggataa ccttaatgca gcgcgaattg
    19561 gcagctcccg cgctattacg caactcattc tcaatctggt agaaggttca ccaattaagc
    19621 taattgatgg cggaaaacaa aaacgctgct ttactgatat ccgggacggt atcgaggcgt
    19681 tgtaccgcat tatcgaaaat gcgggaaatc gctgcgacgg cgaaattatc aacattggca
    19741 atcctgagaa cgaagcgagc attgaggaac tgggcgagat gctgctggcg agcttcgaaa
    19801 aacatccgct gcgccatcac ttcccgccgt ttgcgggctt tcgtgttgtc gaaagtagca
    19861 gctactacgg caaaggatat caggacgtag agcatcgtaa accgagcatc cgcaatgccc
    19921 gccgctgcct ggactgggaa ccgaaaattg atatgcagga aaccatcgac gaaacgctgg
    19981 atttcttcct gcgcaccgtt gatcttacgg ataaaccatc atgaccaaag tcggcttacg
    20041 cattgatgtc gatacctttc gtggcacccg tgaaggcgtg ccacgtctgc tggaaatctt
    20101 gagtaagcat aatattcagg ccagcatttt tttcagcgtc ggcccggaca atatgggccg
    20161 ccatctctgg cgactggtga agccacagtt tttgtggaag atgctgcgct caaacgcggc
    20221 atcgctttat ggctgggata ttttactggc aggtacggcc tggccaggta aagagattgg
    20281 tcatgccaat gccgatatca ttcgtgaagc ggctaaacat cacgaagtcg gcctgcacgc
    20341 ctgggatcac catgcctggc aagcccgtag cggtaactgg gatcggcaaa caatgatcga
    20401 cgatattgca cgcggtcttc gcactctgga agagattatc ggtcaaccgg taacctgttc
    20461 tgccgctgcg ggctggcgtg ccgaccagaa ggtgatcgaa gcaaaagaag cgttccattt
    20521 gcgctacaac agcgattgtc gtggggccat gccgttccgt ccactgctcg aatcaggaaa
    20581 ccctggcact gcgcaaattc cggtgacctt acccacctgg gatgaagtga ttggtcggga
    20641 tgtgaaagca gaagatttta acggttggtt actcaaccgc atcctgcgag ataaaggcac
    20701 gccggtttat accattcatg cagaagttga aggctgcgct tatcagcata attttgtgga
    20761 tctcctcaaa cgcgcagctc aggaaggcgt gacattttgc cctttaagcg aactgttatc
    20821 agagacgttg ccgctcggac aagttgttcg cggaaatatt gccggacgtg aaggctggct
    20881 gggttgccaa caaattgcgg gtagtcgctg atgaaatcgg tacgttacct tatcggcctc
    20941 ttcgcgttta ttgcctgcta ttacctgtta ccgatcagca cgcgtctgct ctggcaaccc
    21001 gatgaaacgc gttatgcgga aatcagtcga gaaatgctgg catccggcga ctggattgtg
    21061 ccccatctgt tagggctacg ttatttcgaa aaacccattg ccggatactg gattaacagc
    21121 attgggcaat ggctatttgg cgcgaataac tttggtgtgc gggcaggcgt tatctttgcg
    21181 accctgttaa ctgccgcgct ggtgacctgg tttactctgc gcttatggcg cgataaacgt
    21241 ctggctctac tcgccacagt aatttatctc tcattgttta ttgtctatgc catcggcact
    21301 tatgccgtgc tcgatccgtt tattgcattc tggctggtgg cgggaatgtg cagcttctgg
    21361 ctggcaatgc aggcacagac gtggaaaggc aaaagcgcag gatttttact gctgggaatc
    21421 acctgcggca tgggggtgat gaccaaaggt tttctcgccc ttgccgtgcc ggtattaagc
    21481 gtgctgccat gggtagcgac gcaaaaacgc tggaaagatc tctttattta cggctggctg
    21541 gcggttatca gttgcgtact gacggttctc ccctggggac tggcgatagc gcagcgggag
    21601 cctgacttct ggcactattt tttctgggtt gagcatattc aacgctttgc actggatgat
    21661 gcccaacata gagctccgtt ctggtactac gtgccggtca tcattgccgg tagcctgccg
    21721 tggctgggat tactccccgg tgcactgtac acaggctgga aaaaccgcaa gcattccgca
    21781 accgtctatt tgttgagctg gacgataatg ccgctgctgt ttttctccgt cgctaaaggt
    21841 aaattgccca cctatattct ttcctgcttt gcatctctgg caatgctgat ggcgcattac
    21901 gctttgctgg cagcaaaaaa taatcctctg gcgctgcgga ttaatggctg gattaacatc
    21961 gcttttggct tcactggcat tattgccaca tttgtggtct ccccgtgggg accaatgaac
    22021 acgccggtgt ggcaaacctt cgagagctat aaagtctttt gtgcctggtc gattttttcg
    22081 ctatgggcat ttttcggctg gtacacctta acaaacgtcg aaaagacctg gtcttttgcc
    22141 gcgctttgcc cgctggggct ggcgttgctg gtaggatttt caattcctga cagagtcatg
    22201 gaaggaaaac atccgcaatt ttttgtcgag atgacacaag aatcacttca gccaagccgc
    22261 tatattctta ctgatagcgt cggtgttgcc gcaggtctgg catggagcct gcaacgcgat
    22321 gacatcatca tgtatcgcca gacaggtgag ttgaaatacg gccttaatta tccggatgcg
    22381 aaagggagat ttgtcagcgg tgatgagttc gcaaactggc ttaatcaaca tcgtcaggag
    22441 gggattatta cactcgtgct ttcggttgac cgcgatgaag atatcaacag tctcgccatt
    22501 ccgcccgcag atgccatcga tcgtcaggag cgtctggtgc tgattcagta tcgtcccaaa
    22561 tgatctggct aacattagtc tttgccagct tgcttagcgt tgccgggcag ttgtgtcaga
    22621 aacaggcaac ctgctttgtg gcgataaaca aacggcgcaa acatatcgtg ctgtggctgg
    22681 gactggcgct ggcttgtctt ggtcttgcca tggtgctctg gctgctggtc ttgcagaacg
    22741 taccggtagg cattgcctac ccgatgttaa gtctgaattt tgtctgggtg acgctggctg
    22801 cagtaaaact gtggcacgaa ccggtatcgc cgcgtcactg gtgtggggtg gcgttcatta
    22861 ttggcggcat tgtgatcctc gggagtacgg tgtaatgggc ctgatgtggg gattattcag
    22921 cgtcattatt gcctcagttg cgcaattaag cctgggtttt gcggcgagtc atctgccgcc
    22981 gatgacgcac ttatgggatt ttatcgcggc tctgttagct tttggcctgg atgcccggat
    23041 actgttactc gggctgctgg gttatctgct ttccgttttt tgttggtata aaacgctaca
    23101 taaacttgcg ctaagcaaag cctatgcctt gctcagtatg agttatgtgc tggtatggat
    23161 tgcttcaatg gtacttcccg gctgggaagg gactttttcg ctgaaagcac tactgggagt
    23221 agcctgtatt atgagcgggt tgatgctgat tttcctgccc acgacaaaac aacgttactg
    23281 agttttccct gccactttac actgccgttc ccactcatcg gggctataac tgcttgccga
    23341 gaggactttc accgtatgca gcttttcacg attaatacaa taaagcgcat tctgcaaagg
    23401 cgagagtaaa tcccccactt tcacggcgaa gtcactcttc acttcggcga tcatttttat
    23461 cgcgccgcca gcatcgcaga gcataagtac atgtctgtta tcttgtttat tgcagcacga
    23521 ttttttgacc agccattcca ttgcattatc ctgtttgcta agagttttca catcaattta
    23581 cagattaaca gataaaccta ttatcaacgg caatggaatg gcaaaattgc gaagtgaaac
    23641 taatgatttc gtaatcctgg cgttgcaccc actcttttaa cgcctgacgt gaaattttaa
    23701 taccgccgtt tttcagctcc ggcggtaaag ttagccagcg caccggttgt tgaaaacgag
    23761 ccagcttatc tttcacccat tcgccaagat caacggtttg ctggtcatac tccaccaccg
    23821 ctaccggtcg atgaccaaac tccttgtcgg caacggggac gataaacacc tgcaaaaccg
    23881 caggatgtgc agcaattacg cgctcgactt cttccggctg aataccctct ccgccactga
    23941 agaacagatt gtctaaacgt ccgacaatgg tcagcttgcc attatgcatc tcaccgcgat
    24001 cgcgcgtagc gtaccagcct tcgtcattaa ccagtgaaac cagttgcccg ttacgccagt
    24061 aaccttctgc catactggca gcccgcagcc acacttcatt attaacgatt ttcacttccc
    24121 gacccggcag cggcgaacca acgtctgcca ggccgtcggc ttctttcgca cacaccgtgg
    24181 aggcaaactc ggtcagacca tagccgcaaa agcaacgaat cccctgctcg cgcgcctgtt
    24241 ccgtcaactc gaccgggata gccgcgccgc caagtaacac cgctttcagg gaaacggaac
    24301 tgcggttaac cagcaaacgc cagagttgtg ttggcaccag tgaagcgtga gtacagcctg
    24361 ccagcatttg ctccaatggc tgtttatcac gcaccgtcat ccgcgcacca gcgtataacc
    24421 agcgccacat aattccctga ccggagacgt gaaacagcgg taaagagagc aaccagtcat
    24481 cgtgatcgcc aaacggaatc agcgataaca caccttgcgc actggcaaga tgggcctgat
    24541 aagtatggac agcggctttc ggcaaaccgg tagaacccga ggtcaacgtc attgagcaca
    24601 gacgcgtcgg ctgccacgta gcggcatgtg cgccttcaac ccgctgaatg tgcagcgacg
    24661 ttaatgccgg aaacgtgttt tccccatccg gcaccagcgc aaattgcagc gtcagattgg
    24721 gcagcaattc ttcaagcaac ggttgcggca gctgagggtt cacgggcaac acccgcgccc
    24781 cgcattgcag taacgccagc caggcgagca gcgtttgcgg cgtattccac gcccgcaaca
    24841 tcacgccgct gccctcaacc acaccctgcg ccgcaaagcc cgtagctaac tcatcgacgc
    24901 gagcacaaag ctcgcgccag ttgagttgct cgtcattaag acgtaaggcg atggcttctc
    24961 cccgcacttg ccgccagtga cgccacggcc agtcagagaa gatcataaca actgctccag
    25021 tacatcagcg tccactaacg gcagtgggct ccccggccag cgacgaacct gctgcgcctg
    25081 catcagatcc agcgtgtcca gtcccggaat ggtgtctggc gttaaccagg cagcaatccg
    25141 cgccagttgc gttagaccta agctcgactc aatggaagaa ctgatcaccg ccgtcagccc
    25201 cagcgcatgc gccgcctgta cctgctcgcg tactttttcc agactgcccg tgagcgtggg
    25261 tttgataact accgcgcgca cgccctcttc agccacaaag gcaaaatccg gctcgcgcag
    25321 gctttcatcc caggcaatgg caatgccggt ttcacgggca aacgctcgcg aatcatcgcg
    25381 ggttttgcac ggctcttcga gaaacgcgat gcggtggcga taatccgggt taacatattt
    25441 ggcaaactgc tgacctttca gcggtgtcca ggcgcgattt gcgtcaagac gcaaatgcag
    25501 atccggaatt gcctccagca acagattaac caccatgccg tcgcgcacgg cttcgtacaa
    25561 tcccactttg accttcgcca ctttctcgcc tggcatatca gcaagtttga ggatcagatc
    25621 gtcaggatcg ccattacaca gtggtgccgc acggtaattg gccgcctgcg ggagcgtatc
    25681 tgccagctcc gccaatgcac agcttacgcc aaaggcaacg gaaggcatct gcggtagctc
    25741 gccatcgcct gccagccagt tatttaccca agcaagcagc acaatttgcg cctcttccca
    25801 ggtttcctga ctgaagcccg gcagtgggga gatctccccc cacccttcgc gctcgccttc
    25861 acgcaggcaa acatacagcc cgtcgcgggt ttttaaccgc ctgtcgcgca gaaccatccc
    25921 cgcgtccatg gggatctgcc agcggtatac ctgcgcgcta cgcattacgg attccgttta
    25981 aatttgctga aatcaggctg acgtttctgg ttgaaagcgt tgcgaccttc ctgaccttct
    26041 tccgtcatgt agaacagcat ggtggcgttg cccgccagct cctgcagacc cgcctgaccg
    26101 tcacagtcgg cgttcagcgc agctttcagg cagcgcagcg ccatcgggct gttttgcagc
    26161 atttcgcggc accaacggac ggtttctttt tccagatccg ccagcggtac cacggtgttg
    26221 acaaggccca tatccagcgc ctgttttgca tcgtactgac ggcacaggaa ccagatttca
    26281 cgcgcttttt tctgtccgac gatgcgagcc atgtaggaag cgccccagcc gccgtcgaag
    26341 gaaccgactt tcgggccagt ctgaccgaag atggcattat ctgccgcgat agtcaggtcg
    26401 cacatcatgt gcagaacgtg accaccgccg atggagtagc cagccaccat cgcgacaacc
    26461 ggtttcggac aggtacggat ctgacgctgg aagtccagca cgttcaggtg atgtacgccg
    26521 gaatcatctt tatagccgcc gtaatcacca cgcactttct ggtcaccacc ggagcagaac
    26581 gctttatcgc ctgcgccagt cagaatgatc acgccgacgt tgtcgtcata acgcgcatct
    26641 gccagtgcct ggatcatctc ttttaccgtc agaggacgga aggcattgcg cacctgcgga
    26701 cgattaatgg tgatttttgc gataccgtcg gtggattttt cataacgaat gtcctcaaaa
    26761 ccttcggagc agtcgtgcca ttcaaccggt gcgtaaagca ttgcttcatc aggataaatc
    26821 atattgtgtc ctttagtcag aaacgcaaga tctgcgccag acttgcgata acgccagcgg
    26881 gattttcccg atgcgcgtta tgtccggcgc gaggaatgac atggcagtcg gcagccagtt
    26941 ccgccgccaa ggcgcggaat ttgctgtcac gttcaccaca taaataataa aacgcaaatg
    27001 tgcgggcgct aaggttagca cgtaaatcag gctggacggc gagagaagtc gcctccagca
    27061 tggcggcaag ggttgcgcca ttattgttac tgcgcagcgc caccagctcc cggcgttgat
    27121 cgtcattgag cgaggcaaaa acaggctgtt gataccagtc ggcaaatacc gccgttaacg
    27181 gttctgtgcg aaaacgctgc gcccattggc gatcggaacg ctgacgttcc gcgcgttgtt
    27241 cagcattttg caacccagga tgcccgcctt cgacaacaac cccacaaagc cccggcatcc
    27301 cctggcaagc cgccatcatc gccacccgtc ccccaagcga gtaccccacc aaccagaagt
    27361 taaggatgtt gtaactaacc agggttttac gcagtaagtc ggtgacatca tcaaatccat
    27421 cgacgctaat cgccgccgaa ccaccgtgac ctgggagata aacatacaac cgtgagtagt
    27481 cggcaaacgc ctcgcccact tcttgccatt cgtggcaatc gccggaaaaa ccgtgcaaaa
    27541 acaccagcca gggtaaacct ggtttgccgt gttttgcctg cgcgtgcagg atcataaatg
    27601 gcttacctgc gccagaagtt gctggagcgt ttgcgcacca tcggtgtcgt taaccaccat
    27661 ttcaattacc gtggtggttg gcgtgcgcca ggcatcggca agtgccgttt caagttcctg
    27721 ccagttttgc ggacgatgat atttcagctc gaacatcgcg gcggcgtgct caaaatggac
    27781 gttttgcggc atcagataga aacgctcgcg ctcgcttttc ggcgttggca gcagcgagaa
    27841 aatttgcccg ccgttgttgt tcaccacaat taatatcagc ggcgcggaaa cctgacgcaa
    27901 taacgccagc gcgttgagat cgtaaagtgc ggagagatcg cccacaatcg ccagcgtcgg
    27961 tttgccgctt gcccgctgaa cgccggcggc agtcgagagc agcccgtcga taccgctggc
    28021 cccacggttg ctgtacaccg ggtaacctgc cggaagttgc gaaagcgcat caatcagacg
    28081 taccaccagg ctgttaccga caaacaattg cccctgttca ggcagatagt cgctgatgcg
    28141 atgcgccagt tgcgcttcgc caaacgcatc gcgacgggca ataaccgcct gcattgcctg
    28201 ttccgccagg cgcgggattt caacgcacca gggctggcgt ttttctgccg gatgcagctc
    28261 cagccagtcg gcaatattgg caattaagcg gcgtccacga tggtgcgccg gatcaagccg
    28321 cccttcgatg tcatccacaa tccagtactc ttcgggttca cagcttgcct gccattgcag
    28381 gagccgtttg cccgtcaggc tgcttcccag ttgcaccaca atttgcgcct gttgcagttc
    28441 gctggtcgct ttggcattgc ctaaccagag atcggcacac ggcagcggct gtccggtttg
    28501 cgacagcacg tcgccaatca gcggccagcc aagagtttgc gcccacagtg caactttttt
    28561 gccctcttcc gcactcatgc gcccggcaac caccacgccg cgtttttgtc gccagaagaa
    28621 ccagtcgcgc tgtttttcac tttccagacg aggcgcttca cgcagccacg gtttgtcgtc
    28681 ctgccaccag tcgcccagcc gctgttgcca gctaagcccg gtgtcgtcca tttcgccata
    28741 cagcggttcg gcaaacgggc agttgatatg gactccacca gaatgaagcg taccgagagc
    28801 atggtcgatg gtagaaacca gccaacgtgc ggggatatcc tgggtcgggc gcggcaatga
    28861 aatactgtgc gtggggtgag aggcgaacat tcccggctga cgaatcgcct gattcgcgcc
    28921 gcagtcaatc agctccggcg ggcgatcggc ggttaacagg atcagttttt ctccggttaa
    28981 cccagcttca atcagtgccg gatagagatt tgctaccgcc gtaccggagg tcacaatcac
    29041 cgccaccggc tgcttgctga ctttcgccag ccccagcgcc agatgcccca acccacgctc
    29101 atcgaaatgg gtgtggtgaa tgaatgcgga attctccgcc gccgctaacg ttaacggtgt
    29161 agaacgcgag ccaggggcga tacagatgtg tctgacgccg tgacgcgtta atgcttccag
    29221 aatgaccgcc gcccagcgtc ggttaaatgc gcttactgac atgagtttgt ccggtatcaa
    29281 tattgcggct aagtataagg agctacaaaa atagattatt gatatgaatc ggtaatgatg
    29341 cgactcatta ctattccatc tgtaataaag tacgcagccc tgccgctttg ttgtcgattt
    29401 cctgccactc ttgctcgggg tcggaaccac ggacaatgcc cgcgccagca tataatcgca
    29461 cgacattgcc gctaattttt gctgagcgca gggaaacgca gaattcgctt tgctgtaatg
    29521 agagatagcc cgcagaaccg gcgtaccatt ctcgggtgaa cggttcgtga cgggcgataa
    29581 actgtcgcgc cagatcgcgc ggtaagccag caactgctgc cgttggctgc aactgatgta
    29641 aacagatcac attatccgct ttgttgagtg aagtccagat acagcggcga agatgctgca
    29701 ctttacgcag acgcagtacc tgcggcggta aaacatccag cgtctgggta tcggcctgta
    29761 accgttggca gatatcttcc accaccagca tattctcgcg ctggttttta tcatccgcca
    29821 tcagccactc acctaactgc tgcgcctgct tatcatcagg atgatttgct accgttcccg
    29881 ccagcgcttc agtacgcagc gctttgtcac gccgccgcca taaccgttcc ggtgaagagc
    29941 caagaaaagc attttcgcca tcaaaggcca tgtaaaaatg gtagcaattc agattcagtc
    30001 gacgactggc agccatcatc gccgccgcgt tgaccggact tgcgaaatgc aggtcagttg
    30061 cccgagcgag caccactttg tcgagctcac cttcggcgat ggttttcgtt gccagttcga
    30121 ttaattgcgt ccagcccgtt ttgtccggcc agtgttgttc tcgcgtggtg gttaaatgta
    30181 acccaggcaa gggcttgata ctcaccagtg tggcgataaa ttctcttgcc ttaatcgcat
    30241 cgtgctgaag ggagctttcg ctgaatagcg tcagccgcag cgtggcttta ccgccacagc
    30301 gtcgccattc caggcgtggt aaaagtaaat tgccctgcga cgggtcaaac gcattcagcc
    30361 cccaaatgcg taagtcggcg tgttccgggt gctggcgaag aaaacgttgt gcctggtcca
    30421 acgacgtaaa acgggtaatc gcgcccagga cggcagcttc ttcatcacca ttacgttgtt
    30481 gccagtagaa ttgcgggtat gtttgctgac tggccagcca gctcaaggca tcaaaagcgt
    30541 ctttgagagg gaaaggaata tcgataaccc gaatgccggg tgtcgccgga atctcttgcg
    30601 acaaatggcg cagtagattt tccagcgccg tagtaagtga ttgcacgcgg acctctccct
    30661 gttaaaagcc tcacattata cggggtacta caaaaaaaat gcagtacccc ggtgtaggga
    30721 ggttttaacg gcgtgccagc aacagtccta gtaccagccc aacggccgcg cccacaccaa
    30781 ttccttgcca gggtttttcg tggacgtagt catcagcacg ataaactgcc tgcttcgccc
    30841 gatagtaata actgtctgaa gcctggctaa cccgtttttt cacatcatcc agcgcttttt
    30901 ctgcacgcgc tttcagctca acatatttct gatcggcggg atcgccagag gagcggagca
    30961 cctcttccag tgtttcacta agcagcgtca ggtcgtcatc gatacgtgta tcaccaaact
    31021 gattagacat tctcgttctc catttgcgta aaacctgtca ctaactatag acaagggttg
    31081 ttgattacgc ctgaattacc tcgcgtgcca tcccaatgtg tgggatacca tcttcttcat
    31141 agacctccgt cacagggata aaaccgaaac tctggtagaa gttttgcaga tgcgcctgcg
    31201 ccccgaggta aacaggctta tcaggccagt gatgcgtgca ggtgtcgagg gttttactca
    31261 tcagttgctg ccccactttt tcgccacgca acgcttcact gacaatcacc cgaccaataa
    31321 cgaccggctc aagatcatca tcacttttca gaatcctcgc atatgccacc agttcatcat
    31381 ttttccaacc gagaatatgg cgattatccc ccgtcaaatc atcaccatcg atatcctgat
    31441 aagggcaatt ctgctcgaca acaaacaccg cgcaacgcag ttgtaataag gcataaagct
    31501 gggaaaccga taattcagag tgatgcagat cttaccattc aatcataatg ctctcctttt
    31561 agatgtatgc cttatactag ccggaatatt ttttgaaacg tgatgaactc atggaattaa
    31621 tttttttagg tacttcagcc ggtgtgccaa cccgcacgcg caatgtcacg gcaatattgc
    31681 ttaacctgca acatccgacc cagagcggac tttggttgtt tgactgcggt gaaggcaccc
    31741 agcatcagct actgcatacc gcctttaacc ctgggaaact ggacaagatt tttatcagtc
    31801 accttcatgg cgatcatctc tttggtttac ccggcttgct gtgcagtcgt tctatgtcag
    31861 gcattatcca acccttaacg atttatggtc cgcaaggtat ccgtgaattt gtggaaaccg
    31921 cgctgcggat tagcggctca tggaccgatt atccgctgga aattgtcgaa attggcgctg
    31981 gcgaaattct cgatgatggc ctgcgcaaag tgaccgctta tccgatggaa cacccactgg
    32041 aatgttatgg ctatcgtatt gaagaacatg ataaaccggg tgcattaaat gcccaggcat
    32101 taaaagctgc tggcgtgccg cctggcccac tgtttcagga attaaaagcg ggcaaaacaa
    32161 tcacgctgga agatggaagg cagattaacg gcgcagatta cttagctgct ccagtgccag
    32221 gtaaagcgct cgctattttc ggcgataccg gcccctgcga tgccgcactt gacctggcta
    32281 aaggtgtcga tgtcatggtg cacgaagcga cgctggatat aaccatggaa gccaaagcca
    32341 atagtcgcgg ccatagctct acacgccagg ctgcgacact agcccgtgag gctggagtcg
    32401 gcaagctaat cattacccac gtcagctcgc gctatgatga caaaggttgt caacacctgt
    32461 tacgtgaatg caggtcaatt ttcccggcga ctgaactggc gaatgatttc accgtgttta
//
LOCUS       DAJVQG010000031        30870 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJVQG010000031 DAJVQG010000000
VERSION     DAJVQG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30870
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.031"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..948
                     /gene="tuf"
                     /locus_tag="OQM83_001310"
     CDS             <1..948
                     /gene="tuf"
                     /locus_tag="OQM83_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1519607.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGD
                     NIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            1178..1561
                     /gene="secE"
                     /locus_tag="OQM83_001311"
     CDS             1178..1561
                     /gene="secE"
                     /locus_tag="OQM83_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCS1519608.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1563..2108
                     /gene="nusG"
                     /locus_tag="OQM83_001312"
     CDS             1563..2108
                     /gene="nusG"
                     /locus_tag="OQM83_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCS1519609.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            2267..2695
                     /gene="rplK"
                     /locus_tag="OQM83_001313"
     CDS             2267..2695
                     /gene="rplK"
                     /locus_tag="OQM83_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCS1519610.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2699..3403
                     /gene="rplA"
                     /locus_tag="OQM83_001314"
     CDS             2699..3403
                     /gene="rplA"
                     /locus_tag="OQM83_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCS1519611.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3816..4313
                     /gene="rplJ"
                     /locus_tag="OQM83_001315"
     CDS             3816..4313
                     /gene="rplJ"
                     /locus_tag="OQM83_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCS1519612.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4380..4745
                     /gene="rplL"
                     /locus_tag="OQM83_001316"
     CDS             4380..4745
                     /gene="rplL"
                     /locus_tag="OQM83_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCS1519613.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            5065..9093
                     /gene="rpoB"
                     /locus_tag="OQM83_001317"
     CDS             5065..9093
                     /gene="rpoB"
                     /locus_tag="OQM83_001317"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCS1519614.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            9170..13393
                     /gene="rpoC"
                     /locus_tag="OQM83_001318"
     CDS             9170..13393
                     /gene="rpoC"
                     /locus_tag="OQM83_001318"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCS1519615.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13635..14768)
                     /gene="thiH"
                     /locus_tag="OQM83_001319"
     CDS             complement(13635..14768)
                     /gene="thiH"
                     /locus_tag="OQM83_001319"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCS1519616.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSHRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14765..15535)
                     /gene="thiG"
                     /locus_tag="OQM83_001320"
     CDS             complement(14765..15535)
                     /gene="thiG"
                     /locus_tag="OQM83_001320"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCS1519617.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15537..15737)
                     /gene="thiS"
                     /locus_tag="OQM83_001321"
     CDS             complement(15537..15737)
                     /gene="thiS"
                     /locus_tag="OQM83_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCS1519618.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15721..16476)
                     /gene="thiF"
                     /locus_tag="OQM83_001322"
     CDS             complement(15721..16476)
                     /gene="thiF"
                     /locus_tag="OQM83_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCS1519619.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16469..17104)
                     /gene="thiE"
                     /locus_tag="OQM83_001323"
     CDS             complement(16469..17104)
                     /gene="thiE"
                     /locus_tag="OQM83_001323"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCS1519620.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17104..18999)
                     /gene="thiC"
                     /locus_tag="OQM83_001324"
     CDS             complement(17104..18999)
                     /gene="thiC"
                     /locus_tag="OQM83_001324"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCS1519621.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAVHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19080..19180)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19233..19709)
                     /gene="rsd"
                     /locus_tag="OQM83_001325"
     CDS             complement(19233..19709)
                     /gene="rsd"
                     /locus_tag="OQM83_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCS1519622.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19804..20577
                     /gene="nudC"
                     /locus_tag="OQM83_001326"
     CDS             19804..20577
                     /gene="nudC"
                     /locus_tag="OQM83_001326"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCS1519623.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20617..21681
                     /gene="hemE"
                     /locus_tag="OQM83_001327"
     CDS             20617..21681
                     /gene="hemE"
                     /locus_tag="OQM83_001327"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCS1519624.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21691..22362
                     /gene="nfi"
                     /locus_tag="OQM83_001328"
     CDS             21691..22362
                     /gene="nfi"
                     /locus_tag="OQM83_001328"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCS1519625.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGVDVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22405..22995
                     /locus_tag="OQM83_001329"
     CDS             22405..22995
                     /locus_tag="OQM83_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCS1519626.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23182..23454
                     /gene="hupA"
                     /locus_tag="OQM83_001330"
     CDS             23182..23454
                     /gene="hupA"
                     /locus_tag="OQM83_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCS1519627.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23467..24162
                     /locus_tag="OQM83_001331"
     CDS             23467..24162
                     /locus_tag="OQM83_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCS1519628.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24164..24589)
                     /gene="zraP"
                     /locus_tag="OQM83_001332"
     CDS             complement(24164..24589)
                     /gene="zraP"
                     /locus_tag="OQM83_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCS1519629.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24827..26203
                     /gene="zraS"
                     /locus_tag="OQM83_001333"
     CDS             24827..26203
                     /gene="zraS"
                     /locus_tag="OQM83_001333"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCS1519630.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            26200..27525
                     /gene="zraR"
                     /locus_tag="OQM83_001334"
     CDS             26200..27525
                     /gene="zraR"
                     /locus_tag="OQM83_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCS1519631.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27522..28811)
                     /gene="purD"
                     /locus_tag="OQM83_001335"
     CDS             complement(27522..28811)
                     /gene="purD"
                     /locus_tag="OQM83_001335"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCS1519632.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28823..30412)
                     /gene="purH"
                     /locus_tag="OQM83_001336"
     CDS             complement(28823..30412)
                     /gene="purH"
                     /locus_tag="OQM83_001336"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCS1519633.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7226 a         8035 c         8519 g         7090 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggtcgt
      721 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      781 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      841 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      901 ggcggccgta ccgttggcgc gggcgttgta gcaaaagttc tgagctaatt gccgataaca
      961 tttgacgcaa tgcgcactaa aagggcatca tttgatgccc tttttgcacg ctttcgtacc
     1021 agaacctggc tcatcagtga ttttctttgt cataatcatt gctgagacag gctctgttga
     1081 gggcgtataa tccgaaaagc taatacgcgt ttcgatttgg tttgcctcgc gatcgcgggg
     1141 tgaaaatgtt tgtagaaaac ttctgacagg ttggtttatg agtgcgaata ccgaagctca
     1201 aggaagcggg cgcggcctgg aagcgatgaa gtgggtcgtt gtggtggcat tgctcctggt
     1261 ggcgattgtc ggcaactacc tttatcgcga cattatgctg ccgctgcgtg cgctggccgt
     1321 agtaattctg attgctgcag cgggtggtgt cgcgctgtta acgacaaaag gtaaagctac
     1381 cgttgctttt gcccgtgaag cgcgtaccga agtccgtaag gtcatttggc cgactcgcca
     1441 ggaaacattg cacaccacgc tgattgtggc tgcggttacc gcagtaatgt cactgatcct
     1501 gtggggactg gatggtattc tggttcgcct ggtatccttt atcactggcc tgaggttctg
     1561 agatgtctga agctcctaaa aagcgctggt acgtcgttca ggcgttttcc ggttttgaag
     1621 gccgcgtagc aacgtcgcta cgtgagcata tcaaattaca caacatggaa gatttgtttg
     1681 gtgaagtcat ggtaccaacc gaagaagtgg ttgaaatccg tggcggtcag cgtcgcaaaa
     1741 gcgaacgtaa attcttccct ggctacgtcc tcgttcagat ggtgatgaac gacgcgagct
     1801 ggcacctggt gcgcagcgta ccgcgtgtga tgggcttcat cggcggtact tccgatcgtc
     1861 ctgcgccaat cagcgataaa gaagtcgatg cgattatgaa ccgcctgcag caggttggtg
     1921 ataagccgcg tccgaaaacg ctgtttgaac cgggtgaaat ggtccgtgtt aatgatggtc
     1981 cgttcgctga cttcaacggt gttgttgaag aagtggatta cgagaaatct cgtctgaaag
     2041 tgtctgtttc tatcttcggt cgtgcgaccc cggtagagct ggacttcagc caggttgaaa
     2101 aagcctaacc cagcgatcaa aaaagcggcg atttaatcgt tgcacaaggc gtgagattgg
     2161 aatacaattt cgcgcctttt gtttttatgg gccttgcccg taaaacgatt ttttatatca
     2221 cggggagcct ctcagaggcg ttattaccca acttgaggaa tttataatgg ctaagaaagt
     2281 acaagcctat gtcaagctgc aggttgcagc tggtatggct aacccgagtc cgccagtagg
     2341 tccggctctg ggtcagcagg gcgtaaacat catggaattc tgcaaagcgt tcaacgcaaa
     2401 aactgattcc atcgaaaaag gtctgccgat tccggtagta atcaccgttt acgctgaccg
     2461 ttctttcact ttcgttacca agactccgcc ggcagcagtt ctgctgaaaa aagcggctgg
     2521 tatcaagtct ggttccggta agccgaacaa agacaaagtg ggtaaaattt cccgcgctca
     2581 gctgcaggaa atcgcgcaga ccaaagctgc cgacatgact ggtgccgaca ttgaagcgat
     2641 gactcgctcc atcgaaggta ctgcacgttc catgggcctg gtagtggagg actaagaaat
     2701 ggctaaactg accaagcgca tgcgtgttat ccgcgagaaa gttgatgcaa ccaaacagta
     2761 cgacatcaac gaagctatcg cactgctgaa agagctggcg actgctaaat tcgtagaaag
     2821 cgtggacgta gctgttaacc tcggcatcga cgctcgtaaa tctgaccaga acgtacgtgg
     2881 tgcaactgta ctgccgcacg gtactggccg ttccgttcgc gtagccgtat ttacccaagg
     2941 tgcaaacgct gaagctgcta aagctgcagg cgcagaactg gtaggtatgg aagatctggc
     3001 tgaccagatc aagaaaggcg aaatgaactt tgacgttgtt attgcttctc cggatgcaat
     3061 gcgcgttgtt ggccagctgg gccaggttct gggtccgcgc ggcctgatgc caaacccgaa
     3121 agtgggtact gtaacgccga acgttgctga agcggttaaa aacgctaaag ctggccaggt
     3181 tcgttaccgt aacgacaaaa acggcatcat ccacaccacc atcggtaaag tggactttga
     3241 cgctgacaaa ctgaaagaaa acctggaagc tctgctggtt gcgctgaaaa aagcaaaacc
     3301 gactcaggcg aaaggcgtgt acatcaagaa agttagcatc tccaccacca tgggtgcagg
     3361 tgttgcagtt gaccaggctg gcctgagcgc ttctgtaaac taatgccttt acgtgggcgg
     3421 tgattttgtc tacaatctta cccccacata taatgcttaa tgcagacgta tatccgagat
     3481 attcgggttg tggcaaggcg gcaactgagt gagtcgccag gagcatagct aactatgtga
     3541 ctggtgcgaa tgaaggaagc caacgccgcc acaagttgaa tagcgacgga tagaaaagat
     3601 ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag accgcaggag tttcgcaaga
     3661 aacttaatcc cctgcgtaga cggtgacaga acgctaagat tattctttta tattctggct
     3721 tgtttctgct caccgtaatt aagacgctct ctccgcttgg aggagtgaag tgagttccag
     3781 agattttctc tggcaaacat ccaggagcaa agctaatggc tttaaatctt caagacaaac
     3841 aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca gtagttgcgg
     3901 attcccgtgg cgtaactgta gataaaatga ctgaactgcg taaagcaggt cgcgaagctg
     3961 gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa ggtactccgt
     4021 tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat tgcatactct atggaacacc
     4081 cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa tttgaggtca
     4141 aagccgctgc ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc ctggcaactc
     4201 tgccgaccta cgaagaagca attgcacgcc tgatggcaac catgaaagaa gcttcggctg
     4261 gtaaactggt tcgtactctg gctgctgtac gcgatgcgaa agaagctgct taatcgcagt
     4321 tatcttttta acgcattcgc ttacgtataa acttattctg atattcagga acaatttaaa
     4381 tgtctatcac taaagatcaa atcattgaag cagttgcagc tatgtctgta atggacgttg
     4441 tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct gctgtagctg
     4501 tagctgctgg cccggttgaa gctgctgaag aaaaaactga attcgacgta attctgaaag
     4561 ctgctggcgc taacaaagtt gctgttatca aagcagtacg tggcgcaact ggcctgggtc
     4621 tgaaagaagc taaagacctg gtagaatctg caccggctgc tctgaaagaa ggcgtgagca
     4681 aagacgacgc agaagcactg aaaaaatccc tggaagaagc tggcgctgaa gttgaagtta
     4741 aataagccaa cccttccggt tgcagcctga gaaatcaggc tgatggctgg tgactttttg
     4801 gtcaccagcc tttttgcgct gtaaggcgcc agtagcgttt cacactgttt gactactgct
     4861 gtgcctttca atgcttgttt ctatcgacga cttaatatac tgcgacagga cgtccgttct
     4921 gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg aaagtgttcc
     4981 attttccggt caacaaaata gtgttgcaca aactgtccgc tcaatggaca gatgggtcga
     5041 cttgtcagcg agctgaggaa ccctatggtt tactcctata ccgagaaaaa acgtattcgt
     5101 aaggattttg gtaaacgtcc acaagttctg gatgtacctt atctcctttc tatccagctt
     5161 gactcgtttc agaaatttat cgagcaagat cctgaagggc agtatggtct ggaagctgct
     5221 ttccgttccg tattcccgat tcagagctac agcggtaatt ccgagctgca atacgtcagc
     5281 taccgccttg gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg cgtgacctat
     5341 tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc gccggaaggc
     5401 accgtaaaag acattaaaga acaagaagtc tacatgggcg aaattccgct catgacagac
     5461 aacggtacct ttgttatcaa cggtactgag cgtgttatcg tttcccagct gcaccgtagt
     5521 ccgggcgtct tctttgactc cgacaaaggt aaaacccact cttcgggtaa agtgctgtat
     5581 aacgcgcgca tcatccctta ccgtggttcc tggctggact tcgaattcga tccgaaggac
     5641 aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat tctgcgcgcc
     5701 ctgaactaca ccacagagca gatcctcgac ctgttctttg aaaaagttat ctttgaaatc
     5761 cgtgataaca agctgcagat ggagctggtg ccggaacgcc tgcgtggtga aactgcatct
     5821 ttcgacatcg aagctaacgg taaagtgtac gtagaaaaag gccgccgtat cactgcgcgc
     5881 cacattcgcc agctggaaaa agacgacgtc aaactgatcg aagtcccggt tgagtacatc
     5941 gcaggtaaag tggttgctaa agactatatt gatgagtcta ccggcgagct gatctgcgca
     6001 gcgaacatgg agctgagcct ggatctgttg gctaagctga gccagtctgg tcacaagcgt
     6061 attgaaacgc tgttcaccaa cgacctggat cacggcccgt atatctctga aaccttacgt
     6121 gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat gatgcgccct
     6181 ggcgagccgc cgactcgtga agcagctgaa agcctgttcg agaacctgtt cttctccgaa
     6241 gaccgttatg acctgtctgc ggttggtcgt atgaagttca accgttctct gctgcgcgaa
     6301 gaaatcgaag gttccggtat cctgagcaaa gacgacatca ttgatgttat gaaaaagctc
     6361 atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg accacctcgg caaccgtcgt
     6421 atccgttctg ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt acgtgtagag
     6481 cgtgcggtga aagagcgtct gtctctgggc gatctggata ccctgatgcc tcaggacatg
     6541 atcaacgcca agccgatttc cgcagcagtg aaagagttct tcggttccag ccagctgtct
     6601 cagtttatgg accagaacaa cccgctgtct gagattacgc acaaacgtcg tatctccgca
     6661 ctcggcccgg gtggtctgac ccgtgaacgt gcaggcttcg aagttcgaga cgtacacccg
     6721 actcattacg gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat cggtctgatc
     6781 aactctttgt ccgtgtacgc acagactaac gaatacggct tccttgagac tccgtatcgt
     6841 aaggtgaccg acggtgttgt aactgacgaa attcactacc tttctgctat cgaagaaggc
     6901 aactacgtta tcgcccaggc gaactccaac ctggatgaag aaggccactt cgtagaagac
     6961 ctggtaactt gccgtagcaa aggcgaatcc agcttgttca gccgtgacca ggttgactac
     7021 atggacgtat ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc gttcctggaa
     7081 cacgatgacg ccaaccgtgc attgatgggt gcgaacatgc aacgtcaggc cgttccgact
     7141 ctgcgcgctg ataagccgct ggttggtact ggtatggaac gtgctgttgc cgttgactcc
     7201 ggtgtaactg cggtagctaa acgtggtggt gtcgttcagt acgtggatgc ttcccgtatc
     7261 gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag caggtatcga catctacaac
     7321 ctgaccaaat acacccgttc taaccagaac acctgtatca accagatgcc gtgtgtgtct
     7381 ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg gtccgtccac cgacctcggt
     7441 gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg ttacaacttc
     7501 gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac caccatccac
     7561 attcaggaac tggcgtgtgt gtcccgtgac accaagctgg ggccggaaga gatcaccgct
     7621 gacatcccga acgtgggtga agctgcgctc tccaaactgg atgaatccgg tatcgtttac
     7681 attggtgcgg aagtgaccgg tggcgacatt ctggttggta aggtaacgcc gaaaggtgaa
     7741 actcagctga ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa agcctctgac
     7801 gttaaagact cttctctgcg cgtaccaaac ggtgtatccg gtacggttat cgacgttcag
     7861 gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga agaaatgcag
     7921 ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc gggtttgttc
     7981 agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct cgacaaactg
     8041 ccgcgcgatc gctggctgga gctgggcctg accgacgaag agaaacaaaa tcagctggaa
     8101 cagctggctg agcagtatga cgaactgaaa cacgagttcg agaagaaact cgaagcgaaa
     8161 cgccgcaaaa tcacccaggg cgacgatctg gcaccgggcg tgctgaagat tgttaaggtg
     8221 tatctggcgg ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg tcacggtaac
     8281 aagggtgtaa tttctaagat caacccgatc gaagatatgc cttacgatga aaacggtacg
     8341 ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat cggtcagatc
     8401 ctcgaaaccc acctgggtat ggctgcgaaa ggtatcggcg acaagatcaa cgccatgctg
     8461 aaacagcagc aagaagtcgc gaaactgcgc gaattcatcc agcgtgcgta cgatctgggc
     8521 gctgacgttc gtcagaaagt tgacctgagt accttcagcg atgaagaagt tatgcgtctg
     8581 gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg tgcgaaagaa
     8641 gcagaaatta aagagctgct gaaacttggc gacctgccga cttccggtca gatccgcctg
     8701 tacgatggtc gcactggtga acagttcgag cgtccggtaa ccgttggtta catgtacatg
     8761 ctgaaactga accacctggt cgacgacaag atgcacgcgc gttccaccgg ttcttacagc
     8821 ctggttactc agcagccgct gggtggtaag gcacagttcg gtggtcagcg tttcggggag
     8881 atggaagtgt gggcgctgga agcatacggc gcagcataca ccctgcagga aatgctcacc
     8941 gttaagtctg atgacgtgaa cggtcgtacc aagatgtata aaaacatcgt ggacggcaac
     9001 catcagatgg agccgggcat gccagaatcc ttcaacgtat tgttgaaaga gattcgttcg
     9061 ctgggtatca acatcgaact ggaagacgag taattctcgc tcaaacaggt cactgctgtc
     9121 ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg agcaaatccg tgaaagattt
     9181 attaaagttt ctgaaagcgc agactaaaac cgaagagttt gatgcgatca aaattgctct
     9241 ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc cggaaaccat
     9301 caactaccgt acgttcaaac cagaacgtga cggccttttc tgcgcccgta tctttgggcc
     9361 ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc gtggcgtcat
     9421 ctgtgagaag tgcggcgttg aagtgaccca gactaaagta cgccgtgagc gtatgggcca
     9481 catcgaactg gcttccccga ctgcgcacat ctggttcctg aaatcgctgc cgtcccgtat
     9541 cggtctgctg ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact ttgaatccta
     9601 tgtggttatc gaaggcggta tgaccaacct ggaacgtcag cagatcctga ctgaagagca
     9661 gtatctggac gcgctggaag agttcggtga cgaattcgac gcgaagatgg gtgcggaagc
     9721 aatccaggct ctgctgaaga gcatggatct ggagcaagag tgcgaacagc tgcgtgaaga
     9781 gctgaacgaa accaactccg aaaccaagcg taaaaagctg accaagcgta tcaaactgct
     9841 ggaagcgttc gttcagtctg gtaacaaacc agagtggatg atcctgaccg ttctgccggt
     9901 actgccgcca gatctgcgtc cactggttcc gctggatggt ggtcgtttcg cgacttctga
     9961 cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac gtctgctgga
    10021 tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg aagcggtaga
    10081 cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca agcgtcctct
    10141 gaaatctttg gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga acctgctcgg
    10201 taagcgtgtt gactactccg gtcgttctgt aatcaccgta ggtccatacc tgcgtctgca
    10261 tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc aaaccgttca tctacggcaa
    10321 gctggaactg cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg ttgagcgcga
    10381 agaagctgtc gtttgggata tcctggacga agttatccgc gaacacccgg tactgctgaa
    10441 ccgtgcaccg actctgcacc gtctgggtat ccaggcattt gaaccggtac tgatcgaagg
    10501 taaagctatc cagctgcacc cgctggtttg tgcggcatat aacgccgact tcgatggtga
    10561 ccagatggct gttcacgtac cgctgacgct ggaagcccag ctggaagcgc gtgcgctgat
    10621 gatgtctacc aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg ttccgtctca
    10681 ggacgttgtt ctgggtctgt actatatgac ccgtgactgt gttaacgcca aaggcgaagg
    10741 catggtgctg actggcccga aagaagcaga acgtctgtat cgctctggtc tggcttctct
    10801 gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa gatgcaaacg gtgaattagt
    10861 agcgaaaacc agcctgaaag acacgactgt tggccgtgcc attctgtgga tgattgtacc
    10921 gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa tctccaaaat
    10981 gctgaacacc tgctaccgca ttctcggtct gaaaccgacc gttatttttg cggaccagat
    11041 catgtacacc ggcttcgcct atgcagcgcg ttctggtgca tctgttggta tcgatgacat
    11101 ggtcatcccg gagaagaaac acgaaatcat ctccgaggca gaagcagaag ttgctgaaat
    11161 tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca aagttatcga
    11221 tatctgggct gcggcgaacg atcgtgtatc caaagcgatg atggataacc tgcaaactga
    11281 aaccgtgatt aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca gcatctacat
    11341 gatggccgac tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg ctggtatgcg
    11401 tggtctgatg gcgaagccgg atggctccat catcgaaacg ccaatcaccg cgaacttccg
    11461 tgaaggtctg aacgtactcc agtacttcat ctccacccac ggtgctcgta aaggtctggc
    11521 ggataccgca ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg ttgacgtggc
    11581 gcaggacctg gtggttaccg aagacgattg tggtacccat gaaggtatca tgatgactcc
    11641 ggttatcgag ggtggtgacg ttaaagagcc gctgcgcgat cgcgtactgg gtcgtgtaac
    11701 tgctgaagac gttctgaagc cgggtactgc tgatatcctc gttccgcgca acacgctgct
    11761 gcacgaacag tggtgtgacc tgctggaaga gaactctgtc gacgcggtaa aagtacgttc
    11821 tgttgtatct tgtgacaccg actttggtgt atgtgcgcac tgctacggtc gtgacctggc
    11881 gcgtggccac atcatcaaca aaggtgaagc aatcggtgtt atcgcggcac agtccatcgg
    11941 tgaaccgggt acacagctga ccatgcgtac gttccacatc ggtggtgcgg catctcgtgc
    12001 ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc atcaagctca gcaacgtgaa
    12061 gtcggttgtg aactccagcg gtaaactggt tatcacttcc cgtaacaccg aactgaaact
    12121 gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagtg ccttacggtg cggtactggc
    12181 gaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg acccgcacac
    12241 catgccggtt atcaccgaag taagcggttt tgtacgcttt actgacatga tcgacggcca
    12301 gaccattact cgtcagaccg acgaactgac cggtctgtct tcgctggtgg ttctggattc
    12361 cgcagaacgt accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg ttgatgctca
    12421 gggtaacgac gttctgatcc caggtaccga tatgcctgcg cagtacttcc tgccgggtaa
    12481 agcgattgtt cagctggaag atggcgtaca gatcagctct ggtgacaccc tggcgcgtat
    12541 tccgcaggaa tccggcggta ccaaggacat caccggtggt ctgccgcgcg ttgcggacct
    12601 gttcgaagct cgtcgtccga aagagccggc aatcctggct gaaatcagcg gtatcgtttc
    12661 cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc accccggtag acggtagcga
    12721 tccgtacgaa gagatgattc cgaaatggcg tcagctcaac gtgttcgaag gtgaacgtgt
    12781 agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg cacgacattc tgcgtctgcg
    12841 tggtgttcat gctgtgactc gttacatcgt taacgaagta caggacgtat accgtctgca
    12901 gggcgttaag attaacgata aacacatcga agttatcgtt cgtcagatgc tgcgtaaagc
    12961 taccatcgtt aacgcgggca gctccgactt cctggaaggc gaacaggttg aatactctcg
    13021 cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa gtgggtgcaa cttactcccg
    13081 cgatctgctg ggtatcacca aagcgtctct ggcaaccgag tccttcatct ccgcggcatc
    13141 gttccaggag accactcgcg tgctgaccga agcagccgtt gcgggcaaac gcgacgaact
    13201 gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta ccggttacgc
    13261 gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg caccgcaggt
    13321 gactgcagaa gacgcatctg ccagcctggc agaactgctg aacgcaggtc tgggcggttc
    13381 tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt
    13441 atgggtgtta ttcagagggc aaattcttca cggaaaatat taagaaaggc agggcgaaac
    13501 gaggaagaag cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca
    13561 caacgctttc aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt
    13621 tcttacattc cgtctcatag tctttgcgag gcgcgtccca gatagctgtc ccagtctttc
    13681 cataccggct gcaaaccctg agcggttaac gcggcagcaa ccgcttccgg tctgcgatcg
    13741 tcgtgcggtg agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt
    13801 ttcgagaagg cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt
    13861 gattcccgcg tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg
    13921 gtttgcacta actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg
    13981 cgcaggcgcg gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc
    14041 aaatgttcgg caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg
    14101 ccgaggccta tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag
    14161 aagaagtcct gttttttgcc tttcaaatga tggcgggtat aagtcgcctc gtgatatgtc
    14221 tcctgataaa ccatcacccc atccagacca agttgcttta actcggcgta ttccgtctcc
    14281 gccagcggtt gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga
    14341 cgacgaaagt aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt
    14401 tcaaagccca tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt
    14461 ttgcgcttga tgcgatgact catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga
    14521 ttggaaagat aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga
    14581 cgctgcgccc gttgggccag tggttccaga tagccactgg cggctggcga taacagcgcc
    14641 atcatatcgt cgcgggtgag ttgcgaggca tttagcgccc gttctacgtc agcagccgtt
    14701 ttgccgttga tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg atcgctgaag
    14761 gttttcatgc cgatgcctcc agaaatccag tcagcgggct ggtggcatga gcaaaatgac
    14821 tgcggctgcc cggtccggac tgacgtgcca gcaggcctgc ttctaccgcc agacgaaatg
    14881 ccttcgccat gttgacggga tcgtccgcta cggcaatcgc cgtattcact aacaccgcgt
    14941 cggcccccat ttccagggcc tgcgcggcat ggctgggaac gccgatgcca gcatcgacaa
    15001 ccaccggaac tgtggcctgc tggataataa tctccagcat ggcacgggtt tccagtccct
    15061 gattcgagcc aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac
    15121 gtttacacaa taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca
    15181 gcatttcggc ggctttcaga gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt
    15241 gaatctctaa ttttaaccag tttgtgccta acgcttcacg tgccagatgg gcggcgaaaa
    15301 tggcttcttc cgctgttttc gccccggaag tatttggcag cagggtcaca cccgccgcga
    15361 taagcggttc gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg
    15421 tcaccagctg gctgccggaa gcgcggatcg cctccaccat cagttgtgaa gaagcgaatt
    15481 tccctgtgcc ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatctcaa
    15541 ccccctgcaa taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgtc
    15601 cactgctcac gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg
    15661 tcgagttgct ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat cggttgatcg
    15721 ttaaacagga tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc
    15781 caggctgcgc cactggctcg atttaccgtc gaacagtcgg agttctcccg caggtgtctc
    15841 tataccgctt aataacttaa tggcttccag tgcctgcaaa gtgcccataa ccccgaccac
    15901 cgggccaacc acgcccgccg tgcggcagtt gcgttctggc tcctggttat ctggccacag
    15961 gcagcggtaa cacccctgct cccagggcgg cgtcagtacc atcaactgac cgccaaatcc
    16021 gaccgcgctg gcggtgataa gcggcgtgtt gagtaccacg caggcggcat taatctcctg
    16081 gcgagtcgcc atattgtcgg tacagtcgag caccacatcg gcccgtgcaa ccgcatcttt
    16141 taacgcctca cccgttaacc gttgttgtaa tgctgtcagt tgaatatcgg gattcaactg
    16201 tgtcagtcgc tgatggctga cctgcgattt cgggcgatcg atatcttcag tggtaaagag
    16261 gatttgtcgt tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc
    16321 gacgccagcc cccgccaggt atagcgcagc aggtgtaccc agcccgccaa gaccaataat
    16381 cagcacctgg ctgtcgagca gtttttgctg cccgtccaga gcgatatcgt cgagcagaat
    16441 ttggcggcta taacgcataa agtcacggtc attcatcgcc aactcctgca atttccagca
    16501 actgtgccgt tgccaaacgc cagtctgcgg cttgagtaat ggcgctgacg acggcgatac
    16561 tgccgacacc cgttgctatc accgcaggcg cgcgcgccag actgataccg ccaatcgcca
    16621 cggtgggata atccgccagt cgctcaacat gccgtgccag ctgttccagc ccctgcggtg
    16681 cagaaggcat ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc
    16741 gtgctgccag cgcgacgtcg atttccatat cgtcatgtgt cgaaacgccc agccgcaggc
    16801 ctgccgcgcg gatggcattg agatcggtgg cttgcaaatc ttcctgcccc aaatggacgc
    16861 catacgcctg atgcttgatc gccagccgcc agtaatcgtt gataaacaat cgcgcgttat
    16921 agcggcggcc cagcgcaatt gccgccacga catcggcttc cacctcttca ttgcgccgat
    16981 ctttgatgcg tagctggagg gtacgtacgc ctgcatccaa cagacgttcg atccactgta
    17041 cgctgtccac caccgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat
    17101 acatcacgct tcctccttac gcaggtagat ttctccgcct ctggcgcgga agttctccga
    17161 catatccgcc attcccactt caatggtttg cgcggcggcg taatcacgta cttcctggct
    17221 gattttcatc gagcagaatt tcggcccaca catggagcaa aaatgggcga ctttacctga
    17281 ctcttgcggc agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt
    17341 aaactggtct tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg
    17401 cgcgcctgga tgccctttcg ccaggtcagc ggcgtggacg gcaatcttat aggtgataag
    17461 cccctgctta acatcttctt tattaggcag acccagatgc tcttttggcg ttacgtaaca
    17521 gagcatcgcg cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc
    17581 atagcccggc gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca
    17641 gtgctctaac tcctcggtca tattgcggcg gatcatctgc atcggcacgt ggcctgggcc
    17701 ttcaatcatc acctgcacgt catattccca ggcaattttg gtcagttcgc ccagcgtatg
    17761 cagctcggca aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc
    17821 gcccagcgac agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg
    17881 atagaggaaa ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg
    17941 agagacgata ccggtcaggc gtttcgcggt catcggcaca tagcgcagca gtacgcccgc
    18001 atggatagtg aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc
    18061 ttcccaggta agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac
    18121 tgtaccgatc ggcaccgggc tgttacgcaa aatccactcg cgggtttcgt gaatatagcg
    18181 accggtggag agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc
    18241 cacttcttct tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac
    18301 caggaaattg cgaccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat
    18361 aatcgcacgt ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag
    18421 atgtgcgcca aagctcattc ccggatgctg gtggcgtaaa acctcgctac ggatgcgctc
    18481 gcggcccata ttctcgcgga tggcgatgaa ttccatttcc ggcgtgatga tgccctggcg
    18541 ggcgtagtgc agttgggtga cacggcgtcc tgttttggcg cgttttggcg ttaataagcc
    18601 gctaaaacgt agctcgtcga gaccatcatc tgccagccgc gttctggtgt aatcggaact
    18661 gcgcacggta agttcttcgg tatcgccgcg cgcatcgatc cacggctggc gtagttttgc
    18721 cagcccttgc tgcacgttaa tggcgatctg cggatcacca tacgggccgg aggtgtcgta
    18781 gaccggaatc gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt
    18841 cgggctaagc tggatctcac gcatcggcac gcgcacgccg gggtgtgtgc cagtgatata
    18901 aatgcgtttt gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcccg
    18961 ggcgcgttgt tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagtcaag
    19021 gacgtggctt gtcagacgac ggatgaagca agagacgatc gccgcaaagg cgatgcgata
    19081 gcagattaac tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa
    19141 taaacggtct cagccagtta aggcactccg acaagaaatt agcccccaca aacggggcgt
    19201 tgaatgtaaa ttacgcgtta acagcgcaga actcaagcag gatgtttgac gcgggcggcg
    19261 tcaagcacca gcagaatcag cttatcttcc agcacaaagc gcgcttccag cgcctcgccg
    19321 atgtcagata aaacctgttg aaactcaagg taattatcat gatcgatggc ggtttccagg
    19381 ctggaatcgt agtaatccat aatctgttgg gtattggctt cgagttgcgg ccaaatcttc
    19441 gcggcgcgcg cgagttgccc gttgccttcc agcttatgaa gaatgcgctc ataaatactg
    19501 aaatgtccgg cagacaagta atcgaccagg ctctgacaaa aatcatcaag ggctttttcg
    19561 tttagcctca tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg
    19621 agcagatgct tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgc
    19681 tccgtcaggt tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact
    19741 gctcagtgag aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga
    19801 gctatggatc gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa
    19861 aaattatggt tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg
    19921 ggtcagcgcg cactacaaat cggcgaatgg cagggggaac ctgtttggtt agtacaacag
    19981 cagcggcgtc acgatatggg gtcggtacgt caggtcattg atctcgatgt tgggctgttt
    20041 caactggccg gacgaggcgt acaactggcg gagttttacc gatcgcataa atactgtggt
    20101 tactgcgggc atgaaatgta tccgagcaaa accgaatggg cgatgctgtg cagccattgc
    20161 cgtgagcgtt actacccgca aatcgccccc tgcattattg ttgccatccg tcgcgatgat
    20221 tcgatcctcc tcgcccagca tacccgccat cgtaacggtg tccatacagt acttgccgga
    20281 ttcgtcgaag tgggcgaaac cctcgagcag gcagtcgcgc gggaagtgat ggaagagagc
    20341 ggaattaaag ttaaaaactt gcgttacgtg acttctcagc cgtggccgtt tcctcagtct
    20401 ttaatgaccg cgtttatggc ggaatatgac agcggcgaca tcgtgatcga cccgaaagaa
    20461 ttgctcgagg cgaactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc
    20521 gtagcgcgcc gtctgataga agatactgtg gcgatgtgtc gggcagagta tgagtgatga
    20581 tacactgacc gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt
    20641 tatctgcggg cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag
    20701 gcgggtcgct atctaccgga atataaagcc acgcgcgccc aggcgggcga ttttatgtcg
    20761 ctgtgcaaaa acgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctacccg
    20821 ctggatgcgg cgatcctctt ttccgatatc ctcaccgtgc cggacgcgat ggggttaggg
    20881 ctctattttg aagccggaga aggtccgcgt tttacctcgc cagtcacctg caaagccgac
    20941 gtcgataaac tgccaattcc ggacccggaa gatgagctgg gttacgtgat gaacgcggtg
    21001 cgtaccattc gtcgcgaact gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg
    21061 tggacgctgg cgacctacat ggtggaaggc ggcagcagca aagcgttcac cgtgatcaaa
    21121 aaaatgatgt atgccgatcc gcaggcgctg cacgctctac tcgataaact ggcgaaaagc
    21181 gtcactttgt atctgaatgc gcagattaaa gccggtgctc aggcagtgat gattttcgac
    21241 acctggggcg gtgtgcttac cgggcgcgat tatcaacagt tctcgctcta ttacatgcat
    21301 aaaattgttg atggtttact gcgtgaaaac gacggtcgcc gcgtgccagt cacgctgttt
    21361 accaaaggcg gcggacagtg gctggaagcg atggcagaaa ccggttgcga tgcgttgggc
    21421 ctcgactgga caacggatat cgccgatgcg cgccgccgtg tgggcaataa agtcgcgttg
    21481 cagggtaata tggatccgtc gatgctgtac gcgccgcctg cccgcattga agaagaagta
    21541 gcgactatac ttgcaggttt cggtcacggc gaaggtcatg tctttaacct tggtcacggc
    21601 attcatcagg atgtgccgcc agaacatgct ggcgtgttcg tggaggcagt gcatcgactg
    21661 tctgaacaat atcaccgcta aggagtgatt atggatctcg cgtcattacg cgctcaacaa
    21721 atcgaactgg cttcttctgt gatccgcgag gatcgactcg ataaagatcc accggatctg
    21781 atcgccggag tcgatgtcgg gtttgagcag ggcggagaag tgacgcgagc ggcgatggtg
    21841 ctgctgaaat atccctcgct tgagctggtg gagtataaag ttgcccgcat cgccaccacc
    21901 atgccttaca ttccaggttt tctttccttc cgcgaatatc ctgcgctgct ggcagcgtgg
    21961 gagatgctgt cgcaaaagcc ggatttagtg tttgtcgatg gtcatgggat ctcgcatcct
    22021 cgccgtcttg gcgtcgctag ccattttggt ttgatggtgg atgtgccaac gatcggcgtg
    22081 gcgaaaaaac ggctctgcgg taaattcgaa ccgctctcca gcgaaccggg cgcgctggcc
    22141 ccactgatgg ataaaggcga gcagctggcc tgggtctggc gcagcaaagc gcgctgtaac
    22201 ccgttgttta tcgctaccgg ccatcgggtc agcgtggaca gcgcgctggc gtgggtacaa
    22261 cgctgcatga aaggctatcg tctgccggag ccaacgcgct gggcggacgc ggtggcctcg
    22321 gaacgtccgg cgttcgtgcg ctatacagca aatcagccct aattcaggta aactgcggat
    22381 aatttccgta tttgagaact caacatgtta caaaacccaa ttcatctgcg tctggagcgc
    22441 ctggaaagct ggcagcatgt cactttcatg gcttgcttat gcgaacgcat gtaccccaat
    22501 tacgccatgt tctgccagca aaccggtttt ggtgatgggc aaatttaccg tcgtattctc
    22561 gatctcatct gggaaacgct gaccgttaaa gacgcaaaag taaatttcga cagccaactg
    22621 gagaaatttg aagaagcgat tccttcagcc gacgatttcg atctgtacgg cgtttatccg
    22681 gcaatcgatg cctgcgtggc gttaagtgaa ctggtccatt cgcgtttgag tggtgaaacg
    22741 ctcgaacacg cggtggaagt gagtaagacc tccatcacga ccgttgcgat gctggaaatg
    22801 actcaggctg gtcgcgaaat gagcgatgaa gagcttaaag aaaacccagc tgtagagcaa
    22861 gaatgggaca ttcagtggga aatattccga cttttagctg agtgcgaaga acgcgatatc
    22921 gagctgataa aaggccttag ggcagacctg cgtgaggcgg gtgagagcaa tattggtata
    22981 atttttcagc aataagacca gaaaacgtga tttaacgcct gatttgtcgt acctggagtc
    23041 ttccctttcg ccccccgtct ggtctacatt tggggggcga aaaaaagtgg ctatcggtgc
    23101 gtgtatgcag gagagtgcta ttctggcatt tccgtcgcac tcgatgctta gcaagcgata
    23161 aacacattgt aaggataact tatgaacaag actcaactga ttgatgtaat tgcagagaaa
    23221 gcagaactgt ccaaaactca ggctaaagct gctctggagt ccactctggc tgcaattact
    23281 gagtctctga aagaaggcga tgctgtacaa ctggttggtt tcggtacctt caaagtgaac
    23341 caccgcgctg agcgtactgg ccgcaacccg cagaccggta aagaaatcaa aattgccgca
    23401 gctaacgtac cggcatttgt ttctggcaag gcactgaaag acgcagttaa gtaagattgc
    23461 gtggcagtga acagttttaa cgaaggggtg gtttcacccc ttttgtcttt ctggcgtcga
    23521 tcattgatgc tggctggcgc tctgcttctc actgcctgta gtcataactc ttcacttcct
    23581 ccctttaccg ccagtggatt tgctgaagac cagggcgcgg tacgcatctg gcgaaaagac
    23641 agcggcgata atgtgcatct gcttgccgtg tttagcccgt ggcgcagtgg cgataccacg
    23701 acgcgagagt atcgctggca gggcgataac ctcacgctca tcaatatcaa tgtttacagc
    23761 aaaccgccgg tgaatattcg cgcgcgtttt gacgatcgcg gtgatctgag ctttatgcaa
    23821 cgtgaatccg atggggaaaa gcagcagctt tctaacgacc aaatcgattt ataccgttat
    23881 cgtgctgatc agatccgcca gattagcgat gccttacgtc aggggagagt cgtgctgcgc
    23941 caggggcgct ggcatgcgat ggaacagacc gtgaccacct gcgaagggca aaccattaaa
    24001 cctgatttag attcgcaggc gatagcgcat atcgagcgcc gccagagccg ctcttctgtt
    24061 gatgtcagcg tggcatggct ggaagcgccc gaaggttcgc aattactgtt agtggcaaac
    24121 tctgatttct gtcgctggca acccaacgag aaaacgttct gatttaccag tggcccatac
    24181 ccatatgacc gccaccaccg cagccgccgt agcccatgcc cattccggct ccgcgcggaa
    24241 tacccgcttc agccatcgcg atatctcgtt tcacccgtaa ctcatctaac gactgacgca
    24301 aattctccat ctctttggcg accgcgttaa ttttgctgct atccggtggg ttcgcggcta
    24361 acagggcatt gtattcataa cgcttcgtca ccagttgctg ttgcagtgcg ctgctttgag
    24421 cgtaaaagtc attatggatt ttctgccacg ctgtctgttg ttcgctggtc aaaggcgcgg
    24481 cattttgctg ccacataccg tgtccgccgt gagcaaatgc agatgtcgat cccatcgcca
    24541 ttgctgaaag cgccatcatt accagggcaa ttttcgtgtt ccgtttcatg gttaatcctc
    24601 cagtggttgt cttacttcgg gtattgcatc ttccgtgcca acgatgaaac gctgatatga
    24661 cgggtaatct ggcatgataa acgagtaaaa atgactcgcc tgctgcgggt agcgagtcat
    24721 ttttactcat tgaaacttaa gcctttgtgt tacagcgcag ggtaagcgct gataaaagat
    24781 ggcatgattt ctgctgtcag aaagggatga gcaggcaaag aagaagatgc gttttatgca
    24841 acgttctaaa gactccttag ctaaatggtt aagcgcgatc ctccccgtgg tcattgttgg
    24901 gctggtaggg ttgtttgcgg tgacggtgat tcgcgattat gggcgcgaga ctgccgccgc
    24961 cagacaaacg ctgctggaaa aaggcagtgt acttattcgc gctcttgaat ccggctcgcg
    25021 cgtcggcatg gggatgcgca tgcatcatgc gcagcagcag gccttactgg aagaaatggc
    25081 cgggcagcct ggagtacgtt ggtttgcggt cacggatgaa caaggaacaa tcgtgatgca
    25141 tagcaactcc ggcatggtgg gaaaacagct ttattccccg caggaaatgc agcagttaca
    25201 tccgggagat gaagaagcgt ggcggcggat cgatagcgca gacggcgagc ctgttctgga
    25261 aatttatcgc cagtttcaac cgatgtttgc tgctggaatg caccggatgc gccatatgca
    25321 gcagtatgcc gcgacaccac aagcaatttt catcgctttc gacgccagta atattgtgag
    25381 tgccgaagat cgtgagcaga gaaacaccct gattatcctc ttcgccctgg cgacggtctt
    25441 gctggcaagc gtattgtcat tcttctggta tcgccgctat ttgcgctcgc gccagcttct
    25501 acaagatgaa atgaagcgca aagagaagct ggtggcgctg gggcatcttg cggcaggcgt
    25561 tgcccacgaa atccgtaacc cactttcctc gattaaagga ctggcgaaat actttgccga
    25621 gcgcgcgcct gcagggggag aagcgcatca actggcgcag gtgatggcga aagaggccga
    25681 ccgtttaaac cgcgtggtaa gcgagctgct ggaactggtt aagccaacgc atctggcttt
    25741 gcaggcggtg gatctcaaca cgctgattaa ccactcatta cagctggtaa gccaggatgc
    25801 gaacagccgg gagatccagt tacgctttac cgccaacgac acattaccgg aaattcaggc
    25861 cgatccggac aggctgactc aggtcctgtt gaatctctat ctcaatgcta ttcaggcgat
    25921 tggtcagcat ggcgtgatta gcgtgacggc cagcgaaagc ggcgcgggcg tgaaaatcag
    25981 cgttaccgac agcggtaagg gaattgcggc agatcagctt gatgccatct tcactccgta
    26041 cttcaccact aaagccgaag gcaccggact ggggctggcg gtcgtgcata atattgttga
    26101 acaacacggt ggtacaattc aggtcgcaag ccaggaggga aaaggcgcaa cgttcaccct
    26161 ctggcttccg gtcactatta cgcgtaaggg cccacaagga tgacgcacga taatatcgat
    26221 attctggtgg tggatgatga cattagccac tgcactattt tgcaggcttt actgcgcggc
    26281 tggggctata acgtcgcgct ggcgaacagc gggcgacagg cgctggagca ggtgcgggaa
    26341 caggtttttg atcttgtgct ttgcgatgtg cgaatggcgg agatggacgg cattgccacg
    26401 ctaaaagaga tcaaagcgtt aaatccggcg attccggtgc tgattatgac cgcatactcc
    26461 agcgtcgaga cggcggtaga agcgttgaaa accggggcgc tggattatct catcaagccg
    26521 ctggatttcg ataacctgca ggcgacgctg gaaaaggcgc tcgcgcatac gcacagtatt
    26581 gacgctgaaa cgcctgcggt gtctgccagc cagttcggta tggtcggtaa aagcccggcg
    26641 atgcaacacc tgctcagtga aatcgccctc gtcgcgccat cggaagccac ggtgctgatc
    26701 cacggcgatt ccggcaccgg taaagagctg gtcgccaggg cgattcacgc cagtagcgca
    26761 cgtagcgaaa aaccgctggt aacgctcaac tgtgcggcac tcaacgaatc cttgctggaa
    26821 tctgaattgt tcggtcacga aaaaggggcg ttcactggag ccgatagacg ccgggaaggg
    26881 cgctttgttg aggcggacgg cggcacgctg tttctcgatg aaattggcga tatctcgccg
    26941 atgatgcagg tgcgtctgct gcgtgcgatt caggagcgcg aagttcagcg tgtcggtagc
    27001 aaccagacta tctcggttga tgtccggttg attgcggcga cccatcgcga tcttgccgca
    27061 gaggtgaatg ccgggcgttt tcgccaggat ctctactatc gcctgaatgt ggtggcgatt
    27121 gaagtaccat cgctgcggca acggcgggaa gatatccctc tgctggctgt ccattttctt
    27181 cagcgctttg ccgagcgtaa tcgcaaggcg gtaaaaggct ttacgccaca ggcgatggat
    27241 ttgctgatcc actacgactg gccgggaaat attcgtgaac tggaaaacgc ggtggaacgg
    27301 gcggtggtgc tgctgaccgg ggaatatatt tccgaacgcg agctgccgct ggcgattgcc
    27361 ggtacgccga tcccactggg gcaaagtcag gatattcagc cgctggtgga agtcgaaaaa
    27421 gaagtgattc tggcggcact ggagaaaacg ggcggcaaca aaaccgaagc cgcccgtcag
    27481 ttagggatca cgcgcaaaac gctattggca aaactgtcgc gttagttctg ctcgcgttcg
    27541 atagcgcgcc agccgatatc tttccggcag aagcagtcgt cccagtgaat atcggtcatt
    27601 aaggcataag cgcgtttctg cgcttctgcc acggtatgac ccagcgcggt gacgcacagt
    27661 acgcgcccgc cgctggtcac tacctgctcg ttatccgcca gtttcgtgcc cgcgtggaac
    27721 actttgccgc ctgccacttc ttccagcggc aggccgtgga tcacatcgcc ggtgcggtaa
    27781 tcgcccggat atccacccgc agccatcacc acgccgagag aagcacgctc atcccactct
    27841 gacgttttct cgtccagttt gccttcacag gccgccaggc agagctcaac cagatcggac
    27901 ttcatgcgca gcataatcgg ctgggtttct ggatcgccaa agcggcagtt aaattcgata
    27961 accttcggat tgccctgttt gtcgatcatc aggcccgcgt agagaaaacc ggtgtaggtg
    28021 ttgccttccg ccgccatgcc tttcacggtt ggccagatga tacgttccat ggtgcgctga
    28081 tgaacttcat cggttactac cggcgcgggg gagtaagcgc ccatcccgcc cgtgtttggt
    28141 ccggtatctt tatcgcctac gcgtttgtga tcctggctgg tggccatcgg cagcacatgc
    28201 tcgccgtcta ccatcacgat aaagctcgct tcttcgccgt cgaggaactc ttcgataacg
    28261 atgcgatgac ccgcgtcgcc aaaagcgttg cccgccagca tatcgtgaac agccgcttcg
    28321 gcttcttcca gcgtcatcgc cacgataacg cctttcccgg ctgccagacc gtccgcttta
    28381 atgacgatcg gcgcaccttt ctcacgcaga tacgccagcg caggttctac ctcggtgaag
    28441 ttctggtatt ccgctgtagg gatcttatgg cgggccagga aatctttggt aaacgctttc
    28501 gaaccttcca gttgcgcggc acctgcggtt gggccgaaga ttttcatccc ggcggcgcgg
    28561 aaggtatcga ccacgccttt caccagcggc gcttccgggc cgacgatggt cagatcaatc
    28621 ttttcgtttt gtgcgaaatc caacagcgcc gggatatcgg tcacgccgat agcaacgttt
    28681 tgcagcgcgg gttccagcgc agtgcctgca ttacccggag caacaaaaac agtttcaacc
    28741 agcggcgact gcgccgcttt ccaggccagc gcgtgctcgc gcccgccgtt accaatcact
    28801 aatactttca tctattgctc cattaatggc ggaagtggcg catgtcggtg aagagcatcg
    28861 caataccgtg ctcgtcggcg gcggcaatca cttcgtcatc acggatagaa ccgccaggct
    28921 ggattacgca ggtcacgcct gcagcggcgg cggcatcaat accgtcgcgg aacgggaaga
    28981 acgcgtcaga agccatcgag gaacctttca cttccaagcc ttcatcggca gctttaatac
    29041 cggcgatttt cgcggagtac acgcggctca tctggcccgc gccaatcccg atggtcatat
    29101 tgtttttggc atagacgata gcgttggatt tcacgaactt cgccactttc cagcagaaca
    29161 gcgcatcacg cagttcctgt tcggtcggct gacgttgggt gacgacgcgc agttcttccg
    29221 caccgaccat gcccaggtca cgatcctgaa ccagcagacc gccgttcacg cgtttgaaat
    29281 caagacccgg aacacgctcg ccccactgac cgcaggtcag aacgcgtacg ttctgcttgg
    29341 cggcggtgat tttcagggct tcttcgctgg cggaaggcgc aataatcact tcgacaaact
    29401 gacgagaaat gatggcctgt gcggtttccg catccagctc gcggttaaag gcaatgatgc
    29461 cgccgaatgc ggaggttggg tcggttttgt acgcgcgatc gtaagcatca agaatggaat
    29521 tgccgatagc cacgccgcaa gggttggcgt gcttcacaat cacacatgcc ggctcggcga
    29581 actctttcac gcactccagc gccgcatcgg tatcggcgat gttgttataa gagagggctt
    29641 taccctgaac ctgggttgcg gtagcaacgg aggcttcttt cacattctct tctatataga
    29701 aggcagcctg ctggtggctg ttctcgccgt aacgcatatc ctgcttctta atgaagttca
    29761 ggttcagcgt gcgtgggaag cgaccggcgg cttctttgct ttcaccgtgg taagccggaa
    29821 ccatgctgcc gaagtagttg gcaatcatgc tgtcgtaggc ggcagtgtgt tcgaaggctt
    29881 tgatggcgag gtcgaaacgg gttgcaagcg tcagcgatcc ttcgttgtca tccatctctt
    29941 taataatggc gtcgtagtcg ctgctcttca ccacgattgc gacatcttta tggttcttag
    30001 cggcggagcg caccatggtc gggccgccga tatcgatgtt ctcaaccgca tcttccagcg
    30061 agcagccttc acgagcaaca gtctgggcga acggatacag gttaacaacc accatatcga
    30121 taggctggat ctgatgttct tccataatgg catcgtcctg gccgcgacgg cccagaatgc
    30181 caccatgtac tttcggatgc agggtcttca cgcgtccatc catcatctcc gggaaaccgg
    30241 tgtaatcgga aacttcggtt accggcagac ctttttctgc taacagacgg gcagtgcccc
    30301 ctgtagacag cagctccaca ccgcgtgcgg aaagtgcctg ggcgaattcg acgataccgg
    30361 ctttgtcaga aacactgagc agagcgcggc ggactggacg acgttgttgc atggtaaatc
    30421 ccctggattt gactattaca gagagcgtta gctgaatttt tcgcgaaaaa ctcagctaac
    30481 gcccctaacg gggcatcctt atttttcgcc cgcattgtaa cgaaaacgtt tgcgcaacgc
    30541 tcgcgaattt ttctctttca atggtgatca caattttgac agtggttacc gtgggaaaaa
    30601 tacagaaatt acattgatga ttgtggataa ctctgtgcgt aaaaaggtat aaagcggggt
    30661 tttgctgggg aatgcagcag tcagtcattt ttctgcaatt tttctattgc ggcctgcgga
    30721 gaaatcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca cacaaacagc
    30781 cggttcggtt gaagagaaaa atcctgaaat tcagggttga ctctgaaaga ggaaagcgta
    30841 atatacgcca cctcgcgaca gtgcgctaaa
//
LOCUS       DAJVQG010000032        30378 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJVQG010000032 DAJVQG010000000
VERSION     DAJVQG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30378
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.032"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(159..371)
                     /gene="rpmE"
                     /locus_tag="OQM83_001337"
     CDS             complement(159..371)
                     /gene="rpmE"
                     /locus_tag="OQM83_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCS1519634.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            574..2772
                     /gene="priA"
                     /locus_tag="OQM83_001338"
     CDS             574..2772
                     /gene="priA"
                     /locus_tag="OQM83_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCS1519635.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2928..3953
                     /gene="cytR"
                     /locus_tag="OQM83_001339"
     CDS             2928..3953
                     /gene="cytR"
                     /locus_tag="OQM83_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCS1519636.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4045..5004
                     /gene="ftsN"
                     /locus_tag="OQM83_001340"
     CDS             4045..5004
                     /gene="ftsN"
                     /locus_tag="OQM83_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCS1519637.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5097..5627
                     /gene="hslV"
                     /locus_tag="OQM83_001341"
     CDS             5097..5627
                     /gene="hslV"
                     /locus_tag="OQM83_001341"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCS1519638.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5637..6968
                     /gene="hslU"
                     /locus_tag="OQM83_001342"
     CDS             5637..6968
                     /gene="hslU"
                     /locus_tag="OQM83_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCS1519639.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7035..7961
                     /gene="menA"
                     /locus_tag="OQM83_001343"
     CDS             7035..7961
                     /gene="menA"
                     /locus_tag="OQM83_001343"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCS1519640.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8054..8539
                     /gene="rraA"
                     /locus_tag="OQM83_001344"
     CDS             8054..8539
                     /gene="rraA"
                     /locus_tag="OQM83_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCS1519641.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8624..8869)
                     /gene="zapB"
                     /locus_tag="OQM83_001345"
     CDS             complement(8624..8869)
                     /gene="zapB"
                     /locus_tag="OQM83_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCS1519642.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9295..10140
                     /gene="glpF"
                     /locus_tag="OQM83_001346"
     CDS             9295..10140
                     /gene="glpF"
                     /locus_tag="OQM83_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HCS1519643.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10163..11674
                     /gene="glpK"
                     /locus_tag="OQM83_001347"
     CDS             10163..11674
                     /gene="glpK"
                     /locus_tag="OQM83_001347"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCS1519644.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            11804..12814
                     /gene="glpX"
                     /locus_tag="OQM83_001348"
     CDS             11804..12814
                     /gene="glpX"
                     /locus_tag="OQM83_001348"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCS1519645.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            12911..13657
                     /gene="fpr"
                     /locus_tag="OQM83_001349"
     CDS             12911..13657
                     /gene="fpr"
                     /locus_tag="OQM83_001349"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCS1519646.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13662..14090)
                     /gene="uspD"
                     /locus_tag="OQM83_001350"
     CDS             complement(13662..14090)
                     /gene="uspD"
                     /locus_tag="OQM83_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCS1519647.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14117..14416)
                     /locus_tag="OQM83_001351"
     CDS             complement(14117..14416)
                     /locus_tag="OQM83_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCS1519648.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14628..15068)
                     /locus_tag="OQM83_001352"
     CDS             complement(14628..15068)
                     /locus_tag="OQM83_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCS1519649.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15169..15768
                     /locus_tag="OQM83_001353"
     CDS             15169..15768
                     /locus_tag="OQM83_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCS1519650.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNSEKGLTFAVEPIKLALSESLEGLNK"
     gene            15876..16643
                     /gene="tpiA"
                     /locus_tag="OQM83_001354"
     CDS             15876..16643
                     /gene="tpiA"
                     /locus_tag="OQM83_001354"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCS1519651.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16698..17453)
                     /gene="cdh"
                     /locus_tag="OQM83_001355"
     CDS             complement(16698..17453)
                     /gene="cdh"
                     /locus_tag="OQM83_001355"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCS1519652.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17560..18549)
                     /gene="sbp"
                     /locus_tag="OQM83_001356"
     CDS             complement(17560..18549)
                     /gene="sbp"
                     /locus_tag="OQM83_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCS1519653.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(18869..19831)
                     /gene="pfkA"
                     /locus_tag="OQM83_001357"
     CDS             complement(18869..19831)
                     /gene="pfkA"
                     /locus_tag="OQM83_001357"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCS1519654.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20012..20914)
                     /gene="fieF"
                     /locus_tag="OQM83_001358"
     CDS             complement(20012..20914)
                     /gene="fieF"
                     /locus_tag="OQM83_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCS1519655.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21062..21562)
                     /gene="cpxP"
                     /locus_tag="OQM83_001359"
     CDS             complement(21062..21562)
                     /gene="cpxP"
                     /locus_tag="OQM83_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCS1519656.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21712..22410
                     /gene="cpxR"
                     /locus_tag="OQM83_001360"
     CDS             21712..22410
                     /gene="cpxR"
                     /locus_tag="OQM83_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCS1519657.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22407..23780
                     /gene="cpxA"
                     /locus_tag="OQM83_001361"
     CDS             22407..23780
                     /gene="cpxA"
                     /locus_tag="OQM83_001361"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCS1519658.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(23886..24560)
                     /gene="yiiM"
                     /locus_tag="OQM83_001362"
     CDS             complement(23886..24560)
                     /gene="yiiM"
                     /locus_tag="OQM83_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCS1519659.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24709..25692)
                     /gene="kdgT"
                     /locus_tag="OQM83_001363"
     CDS             complement(24709..25692)
                     /gene="kdgT"
                     /locus_tag="OQM83_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCS1519660.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(25952..26572)
                     /gene="sodA"
                     /locus_tag="OQM83_001364"
     CDS             complement(25952..26572)
                     /gene="sodA"
                     /locus_tag="OQM83_001364"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCS1519661.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            26857..27891
                     /gene="rhaT"
                     /locus_tag="OQM83_001365"
     CDS             26857..27891
                     /gene="rhaT"
                     /locus_tag="OQM83_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HCS1519662.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(27888..28736)
                     /gene="rhaR"
                     /locus_tag="OQM83_001366"
                     /pseudo
     CDS             complement(27888..28736)
                     /gene="rhaR"
                     /locus_tag="OQM83_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
     gene            complement(28810..29646)
                     /gene="rhaS"
                     /locus_tag="OQM83_001367"
     CDS             complement(28810..29646)
                     /gene="rhaS"
                     /locus_tag="OQM83_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCS1519663.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            29934..>30378
                     /gene="rhaB"
                     /locus_tag="OQM83_001368"
     CDS             29934..>30378
                     /gene="rhaB"
                     /locus_tag="OQM83_001368"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="catalyzes the ATP-dependent phosphorylation of
                     rhamnulose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCS1519664.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQP"
BASE COUNT         7493 a         7836 c         7826 g         7223 t
ORIGIN      
        1 gtaaaatcct tttgaaataa ataagaacca gaagcctagc gccagtcaac aacaggaaat
       61 gataatgagt taacaccccg gaatttttca gaatttataa aaggcaaatt acaggcacaa
      121 aaaaagcgcc gtgcggcgct tttttcggaa atccggtctt atttgctgcc cgggatgttg
      181 aaacgcttgt tgaagcggtc aacacggcca ccggtagcaa catcacgctg tttgccagtg
      241 aagaacgggt ggcacttgct gcacacgtcg aggttcaggt catgaccaac ggtggagcgg
      301 attttcatta cgttaccgca agagcagcta gcagtaattt cttcgtattt cgggtgaata
      361 tcttttttca tgggaaaacc tcggtttaag gccgcgtcgc tcttccagcc ctaacgccag
      421 acaccacgcg atgttaaaag tatagcttca atacgatcat ttcgtacgaa ggcgcgaaat
      481 catacagaaa ttaaccagcg tatgcaaact gatccgcact cttctacggc aatgtgtata
      541 ctaacccacc gaatttcaag tcaggatgat gctatgcccg ttgcccacgt tgccttgccc
      601 gttccgcttc ctcgtacctt tgactatctg ctgccagaag gcatggcggt taaagccggg
      661 tgtcgcgtgc gcgtgccgtt tggcaaacag caggagcgca tcggggttgt ggtatcagtt
      721 agcgatgtca gtgagctgcc gcttaacgag ctgaaagcgg tagttgaagt gctggacgtt
      781 gagccggtat ttactcactc cgtctggcga ttactgctct gggcggcaga ttactatcat
      841 catccgattg gcgatgtgct gtttcatgcc ttgccgattt tactgcgcca ggggcggcct
      901 gcggcgaacg cgccgatgtg gtactggttt gccactgaac aaggccaggc ggtggatctg
      961 aacagcctga aacgctcacc caagcaacaa caggcgctgg cggcgttacg gcaagggaaa
     1021 atctggcgcg accaggttgc cacgctcgaa tttaatgatg ccgcgttgca gacgctgcgc
     1081 aaaaaaggtc tgtgtgattt agcaagtgaa acaccagagt ttagcgactg gcgaacgaac
     1141 tatgccgttt ctggtgagcg gttgcgattg aataccgaac aggccaccgc cgttggcgca
     1201 attcatagcg cggcagatac tttttctgcc tggctgctgg cgggcgttac cggttccggt
     1261 aaaacggagg tttatctcag tgtactggaa aacgtgctcg ctcagggcaa acaggcgctg
     1321 gtgatggtgc cggaaatcgg cctgacaccg caaactatcg cccgttttcg tgaacggttt
     1381 aatgcgcccg tggaagttct gcattccggt ctgaacgaca gcgagcgtct ttcggcgtgg
     1441 ctgaaagcga aaaatggcga ggcggcgatt gtgatcggca cccgctccgc gctgtttacg
     1501 ccgtttaaaa atctcggcgt gattgtcatt gacgaagagc acgacagttc ctacaagcag
     1561 caggaaggct ggcgctatca tgcccgcgac ctggcggtgt atcgtgcgca tagcgagcaa
     1621 atcccgatta ttcttggctc cgcaacgccc gcgctggaaa cgttgtgcaa cgtccagcag
     1681 aaaaaatacc gcctgctgcg cctgacgcgc cgggcgggga atgcgcgtcc ggcaattcaa
     1741 cacgtgctgg atttaaaagg tcagaaggtg caggcaggtc tggctccggc gttaatcacc
     1801 cgtatgcgcc agcatttaca ggccaacaat caggttatcc tctttcttaa ccgccgtggc
     1861 tttgcgcctg cgctactgtg ccacgactgc ggctggattg ccgaatgccc acgttgcgat
     1921 cactactaca cgctgcatca ggcgcaacag catttgcgct gtcaccactg tgacagtcag
     1981 cgtccggtgc cgcgccagtg cccttcctgc ggttccacgc acctggtacc cgtggggctg
     2041 ggcaccgaac agcttgaaca gacgcttgcg ccgctgttcc ctgatgtgcc catttctcgt
     2101 atcgaccgcg ataccaccag ccgcaaaggg gcgctggaac agcaactggc ggaagtacat
     2161 cgcggcggtg cgaggatttt gatcggcaca caaatgctgg cgaaaggtca ccactttccg
     2221 gatgtgacgc tggtggcgtt actggatgtg gacggcgcgc tgttttctgc cgattttcgc
     2281 tcggcagagc gtttcgctca gctttacacc caggtcgccg gtcgtgccgg gcgtgcgggt
     2341 aaacagggcg aagtggtgct gcaaacgcac catccggaac atcctctgtt gcaaacgttg
     2401 ctctataaag gctacgacgc ctttgccgaa caggcgctgg ctgagcgacg catgatgcag
     2461 ctgccgccgt ggaccagcca tgtgattgtg cgtgcggaag atcataacaa tcagcatgcg
     2521 ccgctgttcc tgcaacaact gcgtaatctg atcctctcca gcccgctggc agacgacaaa
     2581 ttgtgggttc tcggtccggt tccggcactg gcacctaaac gtggcggtcg ctggcgctgg
     2641 cagatattgt tgcagcaccc ttcccgcgtg cgcttgcaac acatcattaa cggtacgctg
     2701 gcactcatca atacaatacc ggattcccgt aaggtgaaat gggtgctgga tgttgatccg
     2761 attgagggtt aaaccgctca cgatgcgagg cggatcgaaa aattcaatat tcatcacact
     2821 tttcatgaaa attctgtaac cgttttcacg cgctatctgc taaaaatgtt gccgatgtga
     2881 agtaaacatg gatgtagtac gcctgacgtg ccaggcgagg agtgagtgtg aaagcgaaga
     2941 agcaggaaac tgccgcgacc atgaaagacg ttgccctcaa ggcaaaagtc tctacagcga
     3001 ctgtctcccg agcattaatg aatcccgata aagtctccca ggccacccgt aatcgggttg
     3061 aaaaagcggc ccgggaagtg ggttatttac cgcagcctat ggggcgcaac gtcaagcgta
     3121 atgaatcccg caccattctg gtgattgtcc cggatatctg cgatcccttc tttagcgaaa
     3181 ttattcgcgg tatcgaagtt acggcggcaa atcacggata tctggtgctg attggcgact
     3241 gtgcgcatca aaatcagcag gaaaaaacct ttatcgattt gatcatcacc aagcaaattg
     3301 atggcatgtt gctgctgggt tcaaggctgc cgtttgatgc cagcattgag gaacagcgta
     3361 atctgccgcc gatggtgatg gcgaacgaat tcgcaccgga actggagcta cctacagttc
     3421 atatcgacaa tctgaccgcc gcatttgatg cagtaaatta tctatatgag caagggcata
     3481 aacggattgg ctgtatagcc ggtcccgaag agatgccgct gtgtcactac cgcctgcaag
     3541 gttacgttca ggcgctgcgt cgctgcggca ttatggttga tccgcaatac atcgcccgtg
     3601 gcgacttcac cttcgaagcc ggaagcaaag cgatgcagca gctgcttgat cttccacaac
     3661 cgcctactgc tgtcttctgc catagcgatg tgatggcgct cggcgcactt tctcaggcaa
     3721 aacgccaggg gctgaaagtc ccggaagacc tttccataat cggttttgat aacatcgacc
     3781 tgacgcaatt ttgtgatccg ccgctgacaa ccatcgcgca gccgcgttac gaaatcggtc
     3841 gggaagctat gctgttattg cttgatcaaa tgcaggggca acacgttggc agtggctctc
     3901 gtttaatgga ctgcgaactt atcatccggg gatcaacacg cgcgttacct taaagtaaac
     3961 ctttaagact gccgtgactg gtcaaaggcc cgccgcttaa gtaacatggc gggctgacga
     4021 acgaataaat acagcgaaac gatagtggca caacgagatt atgtacgccg cagccaaccg
     4081 gcaccttcgc ggcgaaaaaa gagcacctca cggaaaaagc aacgaaatct gcctgcggtt
     4141 tctcccgcta tggtcgctat tgctgccgcc gtgcttgtga cctttatcgg tggtctgtac
     4201 ttcattacgc atcacaagaa agaagagtcc gagacgctgc aaagccagaa agtgaccggt
     4261 aacggactac caccaaaacc agaagaacgc tggcgctaca ttaaagagct ggaaagtcgc
     4321 cagccgggtg tgcgtgcgcc cacagaacct tctgccggtg gtgaagtgaa aacgccggag
     4381 caactgacac cagaacaacg tcagcttctt gaacaaatgc aggctgatat gcgccagcag
     4441 ccaacgcagc tggttgaagt tccgtggaat gaacagacgc cggaacagcg tcagcaaacg
     4501 ctacaacgcc agcgtcaggc gcagcaatta gcggagcagc aacgcctggc tcagcagtcg
     4561 cgtacgactg aacaaagctg gcagcagcag acgcgtacgt cgcaagccgc gccggtacag
     4621 gcgcaaccgc gccagtctaa accggcttct actcagcagc cgtaccagga tctgctgcaa
     4681 acccctgcgc acacgaccgc gcagtcgaag ccgcagcaag ctgcgccggt tgctcgtgcc
     4741 gctgacgcgc caaaaccgac ggcggagaaa aaagacgaac gccgctggat ggtgcagtgc
     4801 ggttcgttca gaggcgcgga acaggcagag acggtacgtg ctcagctggc gttcgaagga
     4861 tttgactcga aaatcaccac caacaatggc tggaatcgtg tggtcattgg cccggtgaaa
     4921 ggcaaagaga acgcagacag caccctcaat cggttgaaga tggcgggtca tacaaactgc
     4981 attcggctcg ccgccggggg ttgaaaccct caaaatcccc cccatctata attgcattat
     5041 gccccgtact tttgtacggg gtttgtactc tgtattcgta accaaggggt cagctcgtga
     5101 caactatagt aagcgtacgc cgtaacggcc atgtggtcat cgctggtgat ggtcaggcca
     5161 cgttgggcaa taccgtaatg aaaggcaacg tgaaaaaggt ccgccgtctg tacaacgaca
     5221 aagtcatcgc gggctttgcg ggcggtactg cggatgcttt tacgctgttc gaactgtttg
     5281 aacgtaaact ggaaatgcat cagggccatc tggtcaaagc cgccgttgag ctggcaaaag
     5341 actggcgtac cgatcgcatg ctgcgcaaac ttgaagcact gctggcagtc gcggatgaaa
     5401 ctgcatcgct tatcatcacc ggtaacggtg acgtggtgca gccagaaaac gatcttattg
     5461 ctatcggctc cggcggccct tacgcccagg ctgcggcgcg cgcgctgtta gaaaacactg
     5521 aacttagcgc ccgtgaaatt gctgaaaagg cgttggatat tgcaggcgac atttgcatct
     5581 ataccaacca tttccacacc atcgaagaat taagctacaa agcgtaagga tctcccatgt
     5641 ctgaaatgac cccacgcgaa atcgtcagcg aactggataa gcacatcatc ggccaggaca
     5701 acgccaagcg ttctgtggcg attgctctgc gtaaccgctg gcgtcgcatg cagctcaacg
     5761 aagagctgcg ccatgaagtg accccgaaaa atatcctgat gatcggcccg accggtgtcg
     5821 gtaaaactga aatcgcccgt cgtctggcta agctggcgaa tgcgccgttc atcaaagttg
     5881 aagcgaccaa attcaccgaa gtgggctatg tcggtaagga agtggattct attattcgcg
     5941 atctgaccga tgccgccgtg aaaatggtac gcgtccaggc tatcgagaaa aaccgttatc
     6001 gcgctgaaga actggcagaa gaacgtattc tcgacgtgct gatcccacct gctaaaaaca
     6061 actggggaca gaccgaacag cagcaggaac cgtccgctgc tcgtcaggca ttccgcaaaa
     6121 aactgcgtga aggccagctt gatgacaaag aaatcgagat cgatcttgcc gcagcaccga
     6181 tgggcgttga aattatggct cctccaggca tggaagagat gaccagccag ctgcagtcca
     6241 tgttccagaa cctgggcggc cagaagcaaa aagcgcgtaa gctgaaaatc aaagacgcca
     6301 tgaagctgct gattgaagaa gaagcggcga aactggtgaa cccggaagag ctgaagcaag
     6361 acgctatcga cgctgttgag cagcacggga tcgtgtttat cgacgaaatc gacaaaatct
     6421 gtaagcgcgg cgagtcttcc ggtccggatg tttctcgtga aggcgttcag cgtgacctgc
     6481 tgccgctggt agaaggttgc accgtttcca ccaaacacgg gatggtcaaa actgaccaca
     6541 ttctgtttat cgcttctggc gcgttccaga ttgcgaaacc gtctgacctg atcccggaac
     6601 tgcaaggtcg gctgccaatc cgcgttgaac tgcaggcact gaccaccagc gacttcgagc
     6661 gtattctgac cgagccgaat gcctctatca ccgtgcagta caaagcactg atggcgactg
     6721 aaggcgtaaa tatcgagttt accgactccg gtattaagcg catcgcggaa gcggcatggc
     6781 aggtgaacga atctaccgaa aacatcggtg ctcgtcgttt acacactgtt ctggagcgtt
     6841 taatggaaga gatttcctac gacgccagcg atttaagcgg tcaaaatatc actattgacg
     6901 cagattatgt gagcaaacat ctggatgcgt tggtggcaga tgaagatctg agccgtttta
     6961 tcctataatc gcgttcaatc attttcatca ttgtttgatg gggctgaaag gccccatttt
     7021 tattggcgcg tattatgact gaacaacaaa ttagccgaac tcaggcgtgg ctggaaagtt
     7081 tacgacctaa aaccctcccc ctcgcctttg ctgcaattat cgtcgggacg gcgctggcat
     7141 ggtggcaagg tcacttcgat ccgctggtcg ccctgctggt actaattacc gccgggctat
     7201 tacagatcct ttctaacctc gccaatgatt acggcgatgc ggtaaaaggc agcgataaac
     7261 ctgaccgcat tgggccgcta cgcggcatgc aaaaaggggt cattacccag caagagatga
     7321 aacgggcgct cattattacc gtcgtgctca tctgtctctc cgggctggca ctggttgcag
     7381 tggcatgcca tacgctggcc gattttgtcg gtttcctgat tcttggcggg ttgtcgatca
     7441 ttgccgctat cacctacacc gtgggcaatc gtccttatgg ttatatcggt ctgggtgata
     7501 tttccgtact ggttttcttt ggctggttga gcgtcatggg gagctggtat ttacaggctc
     7561 atacattgat tccggcactg atccttccgg cgaccgcgtg tggcctgctg gcaacggcag
     7621 tactgaacat taataacctg cgtgatatca atagcgaccg cgaaaatggc aaaaacacgc
     7681 tggtggtgcg cttaggtgaa gtgaacgcgc gtcgttatca tgcctgcctg ctgatgggct
     7741 ctctggtatg tctggcgctg tttaatctct tttcgctgca tagcctgtgg ggctggctgt
     7801 tcctgctggc ggcaccatta ctggtgaagc aagcccgtta tgtgatgcgg gaaatggacc
     7861 cggtggcgat gcgaccaatg ctggaacgta ctgtcaaggg agcgttactg actaacctgc
     7921 tgtttgtttt agggatattc ctaagccagt gggcagcata actgacaaat atcaattaac
     7981 aattgatgat tttgccaaca gcctacatag cgcgatatac tgaaaattct cgcagcaact
     8041 gaatgttaag cctatgaaat acgatacttc cgagctttgt gacatctatc aagaagatgt
     8101 taacgtcgtg gaaccgctgt tctccaactt tggcggacgg gcgtcgtttg gcggacaaat
     8161 aatcacggta aaatgtttcg aggacaacgg gttgctgtac gatctgctcg aacagaatgg
     8221 ccgtggtcgt gttcttgtcg tcgatggcgg tggttctgtt cgtcgcgcac tggtcgatgc
     8281 tgaactggcg cgtctggcag tacaaaatga atgggaaggt ctggttattt acggcgcggt
     8341 gcgtcaggta gatgacctag aagagttgga tatcggcatc caggcgatgg cggcaattcc
     8401 ggttggtgcc gctggcgaag gcattggcga aagcgatgtc cgcgtcaatt ttggcggtgt
     8461 aaccttcttc tccggcgacc atctttatgc cgacaatacc gggattattc tttcagaaga
     8521 tccgctggat attgaatgat aagaaaggca ccgcaaggtg ccttttttct gcgttacctg
     8581 ttggcctaca cagtaaagaa attacgcgaa agatgaagcg taatcagacc tcttccatgc
     8641 gacccagcag ggcctgcaga cgttcctgcc agccgttctg ctgttctttc agatggttgt
     8701 tctcacgctc cagctcttcg cgctgatgct gggcattttg aacttcctgc gacagtgagt
     8761 tgtttttttc tttcagctct tcgatttcca tctgcaacag agtgatggta tcaatcgcct
     8821 gctgtacttt tgcttccagt ttctcaaaca cttctaatga cattgtcata cctctcctga
     8881 attgcaaggc gttgatggat aaaaaatcct cgtcccgatt accggtgacg ccttaataaa
     8941 tacgagcgca ctttagttag ctccgattgt atgaagccgc gccatcgctg tccagcggca
     9001 cgccttgcag attacggttt gccacacttt tcatccttct cctggtgaca taatccacat
     9061 caatcgaaaa tgttaataaa tttgttgcgc gaatgatcta acaaacatgc atcatgtaca
     9121 atcagatgga ataaattgcg cgataacgct cattttatga cgaagcacac acattttaag
     9181 ttcgatattt ctcgtttttg ctcgttaacg ataagtttac agcatgccta caagcatcgt
     9241 ggaggtccgt gactttcacg catacaacaa acattaactc ttcaggatcc gattatgagt
     9301 caaacatcaa ccttgaaagg ccagtgcatt gctgaattcc tcggtaccgg gttgttgatt
     9361 tttttcggtg tgggttgcgt tgcagcacta aaagtcgctg gtgcgtcttt tggtcagtgg
     9421 gaaatcagtg tcatttgggg actgggggtg gcaatggcca tctacctgac cgcaggggtt
     9481 tccggcgcgc atcttaatcc cgctgttacc attgcattgt ggctgtttgc ctgtttcgac
     9541 aagcgcaaag ttattccttt tatcgtttca caagttgccg gcgctttctg tgctgcggct
     9601 ttagtttacg ggctttacta caatttattt ttcgacttcg agcagactca tcacattgtt
     9661 cgcggcagtg ttgaaagtgt tgatctggca ggcactttct ctacttaccc taatcctcat
     9721 atcaattttg tgcaggcttt cgcagttgag atggtgatta ccgctattct gatggggctg
     9781 atcctggcgt taacggacga tggcaacggt gtaccacgcg gccctttggc tcccttgctg
     9841 attggtctac tgattgcggt cattggcgca tctatgggcc cattgacggg ttttgccatg
     9901 aacccagcgc gtgacttcgg tccgaaagtc tttgcctggc tggcgggctg gggcaatgtc
     9961 gcctttaccg gcggcagaga cattccttac ttcctggtgc cgctttttgg ccctatcgtt
    10021 ggcgcgattg taggtgcatt tgcctaccgc aaactgattg gtcgccattt gccttgcgat
    10081 atctgtgttg tggaagaaaa ggagaccaca actccttcag aacaaaaagc ttcgctgtaa
    10141 tatgactacg ggacaattaa acatgactga aaaaaaatat atcgttgcgc tcgaccaggg
    10201 caccaccagc tcccgcgcgg tcgtaatgga tcacgatgcc aatatcatta gcgtgtcgca
    10261 gcgcgaattt gagcaaatct acccaaaacc aggctgggta gaacacgacc caatggaaat
    10321 ctgggccacc caaagctcca cgctggtaga agtgctggcg aaagccgata tcagttccga
    10381 tcaaattgca gctatcggta ttacgaacca gcgtgaaacc actattgtct gggaaaaaga
    10441 aaccggcaag cctatctata acgccattgt ctggcagtgc cgtcgtaccg cagaaatctg
    10501 cgagcattta aaacgtgacg gtttagaaga ttatatccgc agcaataccg gtctggtgat
    10561 tgacccgtac ttttctggca ccaaagtgaa gtggatcctc gaccatgtgg aaggctctcg
    10621 cgagcgtgcg cgtcgtggtg aattgctgtt tggtacggtt gatacgtggc ttatctggaa
    10681 aatgactcag ggccgtgtcc atgtgaccga ttacaccaac gcctctcgta ccatgttgtt
    10741 caacatccat accctggact gggacgacaa aatgctggaa gtgctggata ttccgcgcga
    10801 gatgctgcca gaagtgcgtc gttcttccga agtgtacggt cagactaaca ttggcggcaa
    10861 aggcggcacg cgtattccaa tctccgggat cgccggtgac cagcaggccg cgctgtttgg
    10921 tcagttgtgc gtgaaagaag ggatggcgaa gaacacctat ggcactggct gctttatgct
    10981 gatgaacacc ggcgagaaag cggtgaaatc agaaaacggc ctgctgacca ccatcgcctg
    11041 cggcccgact ggcgaagtga actatgcgct ggaaggtgcg gtgtttatgg caggcgcatc
    11101 cattcagtgg ctgcgcgatg agatgaagtt gattaacgac gcctacgatt ccgaatattt
    11161 cgccaccaaa gtgcaaaaca ccaatggtgt gtatgtggtt ccggccttta ccgggctggg
    11221 tgcgccgtac tgggacccgt atgcgcgcgg ggcgattttc ggtctgactc gtggggtgaa
    11281 cgctaaccac attatacgcg cgacgctgga gtctattgct tatcagacgc gtgacgtgct
    11341 ggaagcgatg caggccgact ctggtatccg tctgcacgcc ctgcgcgtgg atggtggcgc
    11401 agtagcaaac aatttcctga tgcagttcca gtccgatatt ctcggcaccc gcgttgagcg
    11461 cccggaagtt cgcgaagtga ctgcattggg cgcggcctat cttgctggtc tggcggtcgg
    11521 tttctggcag aacctcgacg agctgcaaga gaaagcggtg attgagcgcg agttccgtcc
    11581 aggcatcgaa accactgagc gtaattaccg ttacgcaggc tggaaaaaag cggttaaacg
    11641 cgcgatggcg tgggaagaac acgacgtaat gtaaatgccg gatgaagcgt ttatgccgca
    11701 tccggtagtc ccgaaacgtg cgggggcaac cccgcacaca tcaataatcc ctcccttccc
    11761 ctgtgctaca cttcgcgcca ttccttactg cttagagttt gctatgagac gagaacttgc
    11821 catcgaattt tcccgcgtca ccgaatctgc ggcgctggct ggctacaaat ggttaggacg
    11881 cggcgataaa aacaccgcgg acggcgcggc ggtaaacgcc atgcgtatta tgctcaacca
    11941 ggtcaatatt gacggcacca tcgtcattgg tgaaggtgaa atcgacgaag caccgatgct
    12001 ctacattggt gaaaaagtcg gtactggtcg cggcgacgcg gtagatattg ctgttgatcc
    12061 gattgaaggc acgcgcatga cggcgatggg ccaggctaac gcgctggcgg tgctggcagt
    12121 aggcgataaa ggctgcttcc tcaatgcgcc ggatatgtat atggagaagc tgatcgtcgg
    12181 accgggagcc aaaggcacca ttgatttgaa cctgccgctg gcggataacc tgcgcaatgt
    12241 tgcggcggcg ctcggcaaac cgttgagcga actgacggta acaattctgg ctaaaccacg
    12301 ccacgatgcc gttatcgctg aaatgcaaca actcggcgta cgcgtatttg ctattccgga
    12361 tggcgacgtt gcggcctcaa ttctcacctg tatgccagac agcgaagttg acgtgctgta
    12421 cggtattggt ggcgcgccgg aaggcgtagt ttctgcggcg gtgatccgcg cattagatgg
    12481 cgacatgaac ggtcgtctgc tggcgcgtca tgacgtcaaa ggcgacaacg aagagaatcg
    12541 tcgcattggc gagcaggagc tggcacgctg caaagcgatg ggcatcgaag ccggtaaagt
    12601 attgcgcctg ggcgatatgg cgcgcagcga taacgtcatc ttctctgcca ccggtattac
    12661 caaaggcgat ctgctggaag gcattagccg caaaggcaat atcgcgacta ccgaaacgct
    12721 gctgatccgc ggcaagtcac gcaccattcg ccgcattcag tctatccact atctggatcg
    12781 caaggacccg gaaatgcagg tgcacatcct ctgattgatt tgatcgattg agccttccag
    12841 tccttcggga ctggaatttt tttgttcgga gaacgaagat aaggcaagtc aatcaaaaca
    12901 ggagaaaaac atggctgatt gggtaacagg caaagtcact aaagtgcaga actggaccga
    12961 cgccctgttt agtctcaccg ttcacgcccc cgtgcttccg tttaccgccg ggcaattcac
    13021 caagcttggc cttgaaatcg acggcgaacg cgtccagcgc gcctactcct atgttaactc
    13081 gcccgataat cccgatctgg agttttacct ggtcaccgtc cccgatggca aattaagccc
    13141 acgactggcg gcactgaaac caggcgatga agtgcaggtg gttagcgaag cagctggctt
    13201 ctttgttctg gaggaagtac cagattgcga aacgctatgg atgctggcaa ccggtacagc
    13261 gattggccct tatttatcga ttctgcaact aggcaaagat ttagatcgct tcaaaaatct
    13321 ggtcctggtt cacgccgcac gttatgccgc cgacttaagc tatttgccac tgatgcaaga
    13381 actggaaaaa cgctacgaag gcaaactgcg cattcagacg gtggtcagtc gggaaacggc
    13441 agcggggtcg ctcaccggac ggataccggc attaattgaa agtggggaac tggaaagcgc
    13501 gattggcctg ccgatgaata aagaaaccag ccatgtgatg ctgtgcggca atccacagat
    13561 ggtgcgcgat acacaacagt tgctgaaaga gacccggcag atgacgaaac atttacgtcg
    13621 ccgaccgggc catatgacag cggagcatta ctggtaagcg gttacttatc gataaacggc
    13681 acgatgagca aatccgcact catcttattg atcatcccgc gatatgccgg catcaaacgg
    13741 ttgataaatg agtggtgatg accacagaca aggaggtcgc actgctcttt ttgcataatt
    13801 tccagcagtg tttccggcat ttctccgcgt tcaatacgca gttttgtctt cggccattga
    13861 atatttttcg tcagtttata cagcttgtta tccgacttat tcttcaacaa ttgaagaata
    13921 tcttctgttg cagggaagta gatacccgga tacaactcgc ttaagccatc atcaatatga
    13981 attaacgtca ggtgagcgtc attatgtctg gcgagctcca gggctttatt caccagtaag
    14041 gcatcttctt cattcccgga aattgccacg ccaatgtgtt tataagccat gtttaactcc
    14101 ttctaaagca actccatcaa gctaacgaac gcagtgataa ctcaaaaata atcatctcgg
    14161 cctggcatga gaaagtgaag gccgcatcaa gctcaacttg cccctccaga tccttaatct
    14221 tataggccac gctggcccct tcatctgcag aggatgcagc ggcggcaagc tctttcagtt
    14281 tggtcataaa agactctgct tcttcgcggg tggcaaaaaa gcgcgaaaac ttactggtac
    14341 agttgtcgtt atcaatcacc gtaccgatat caatcgcaca tcctttagta ctgcatttat
    14401 ctacgacatc tttcataggg cacctctgta tattcgcctc tctgtcgatt acgacttaag
    14461 aataatgcgc cgttaaggga agaacaaaca agtaatgatc ttatggatta ccattttttt
    14521 tgatattgct taaggtgaag agttcagaag gattttccgg gtattgtcat ttgcatcgtg
    14581 gaaataacaa tcccggaaaa aaatagcagt aagagatata ttaaaggtta attacttgat
    14641 ttattgtcgg ctttatactt cacatcctga gtatctttac catatttatt ttcgccttgt
    14701 gtgccaacaa acgcaccaag atcgataagc atcatcacca gaatcaacgt cgggacaaaa
    14761 cgccccaccg cccattgcca gacacccggt aaaatcgccc agttacccgc cagcagcatc
    14821 cacgccacaa tcatcagaaa tgcccatgcg ccggaacgcc cgcgatcatg caagcgctta
    14881 acagttactg ccgctgttgg ccaaagcaag cacacaaggc aaaacgccgc ggtctgaata
    14941 tcgagtaaat ttttacccgc cagtgagaaa agcaccagca tgcctgcgaa ccacaggcct
    15001 atccaaatcc agaaatcacg gcgtccaata cgccctttaa atgagaataa ccattgctgt
    15061 atggtcatgt aagttccttg atggttgtct tttccaggat tctacccttt tgacaagggc
    15121 gacagatatc gttttaatcg gagccagtca taacaaaagg tactgtcaat gaagccaggg
    15181 tgtacgctgt tttttctctt atgttctgca ttaaccgtta caacaacggc gcatgcacaa
    15241 acaccagata cggcaacgac cgcgccttat ctgctggctg gagcccctac tttcgatctc
    15301 tccatcagcc agtttcgaga agactttaac agccagaatc ctagcctgcc actgaacgaa
    15361 tttcgtgcca tcgacagcag tcccgacaaa gccaatctca ctcgtgctgc aagtaaaatt
    15421 aacgagaact tgtatgcttc tacagcgctg gagcgcggta ccttaaaaat caaaagcatt
    15481 caaatgacct ggctacccat ccaggggcca gagcaaaaag cagcgaaagc gaaagctcag
    15541 gaatacatgg cagcagtgat ccgcacactc accccattaa tgaccaaaac acaaagccag
    15601 aaaaaactgc agtcgctact aacggcgggg aaaaacaaac gttattacac cgagacagaa
    15661 ggtgcactgc gttatgttgt cgcggacaac agcgaaaagg ggctgacctt cgctgttgaa
    15721 ccgattaagc tggcgctatc tgaatcgctt gaaggtttga ataaatgaca aaaagcaaag
    15781 cctttgtgcc gatgaatctc tatactgttt cacagacctg ctgccctgcg gggcggccat
    15841 cttcctttat tcgcttataa gcgtggagaa ttaaaatgcg acatccttta gtgatgggta
    15901 actggaaact gaacggcagc cgccacatgg ttcacgagct ggtttctaac ctgcgtaaag
    15961 agctggcagg tgttgctggc tgtgcggttg caatcgcacc accggaaatg tacatcgata
    16021 tggcgaagcg cgaagctgaa ggcagccaca tcatgctggg tgcgcaaaac gtggacctga
    16081 acctgtccgg cgcattcacc ggtgaaactt ctgccgctat gctgaaagac atcggcgctc
    16141 agtacatcat catcggccac tctgaacgtc gtacttacca caaagagtct gacgaactga
    16201 tcgcgaaaaa attcgcggtg ctgaaagagc agggcctgac tccggttctg tgcatcggtg
    16261 aaaccgaagc tgaaaacgaa gcgggcaaaa ctgaagaagt ttgcgcacgt cagatcgacg
    16321 cggtactgaa aactcagggt gctgcggcat tcgaaggtgc ggttatcgct tacgaacctg
    16381 tatgggcaat cggtactggc aaatctgcaa ctccggctca ggcacaggct gttcacaaat
    16441 tcatccgtga ccacatcgct aaagttgacg ctaacatcgc tgaacaagtg atcattcagt
    16501 acggcggctc tgtaaacgcg tctaacgctg cagaactgtt tgctcagcca gatatcgacg
    16561 gcgcgctggt tggcggtgct tctctgaaag ctgacgcttt cgcagtgatc gttaaagctg
    16621 cagaagcggc taaacaggct taagtctgac aggtgccgga tttcatatcc ggcacttact
    16681 ttccttaact cttcgcctta acgcaaaatc tcacactgat gatcctgaat ttcctcggct
    16741 gaagcacggt taagcgtcag tagatttcgt tgtgtcgcca gcaatacaaa tgagttatca
    16801 ctctgccgta ccatcgccag cccgtagctt cccatatgtt cccgcgcctc aggtacttct
    16861 tctgccagca tcataaatgg gctgcgttgt accagttcgc tttccgttac ccgacgcgcc
    16921 aggtattcat gcccacgcaa accacctggc agtggcaacc agcggctgct gatgttcgcc
    16981 agattgttat ccagttgttc gcgcacatca ggacgaatac aagagatatg aatatgaaaa
    17041 tggttttgcg tacgcccggt gcgggagttg atcgccaaag aaaccgcgcg atcgggaacc
    17101 ggctggccgt attttttgct cataaaatca cgcgcctgcc aggccaacca aaagaagttc
    17161 ggcgttgaag gatcggtcaa caaaggactt tcagtaccgt taatacgata cgttggcatc
    17221 aacagatatt gcagtggacc attaagatct tttaaaacca cgtatccggc attgggtttg
    17281 acttccgcac atggcgaagg attttgattt tgctgctgat tgggcaaaca ttcctcaagg
    17341 acaatcttac gtaatgtatc cgactcttca ccggttaatt tccagtaacc aataccggca
    17401 gccacaacgg cgataactat catcaccaaa aaaagaagac ccgctttttt catctttttt
    17461 tccctgtacc tcaaagagat gcaagggtaa cgcaaaatcg tgacaaataa aaaacccggt
    17521 cgcgagaaac caccgggttt gataattatc ctggggaaat cagcgtttgc tgatctgatc
    17581 gaacgtaccg ccgttagcaa aatgctcttt ttgcgctttc gtccagccgc cgaactcttc
    17641 atcaatggtg aataacttca gctttggaaa cgcattttcg tactttttcg ctacttcagc
    17701 gtcgcgcgga cggtagtagt ttttcgcggc aatttcctga ccttctggcg agtagagata
    17761 tttcaggtag gcttccgcca cctctttggt gccttttttc tcgaccactt tatcgaccac
    17821 cgacacggtt ggctctgcga ggatagactc actcggcgtg acgatttcga atttatcttt
    17881 ccccagttca ttcgctgcca gcagagcttc gttttcccag gcgatcagta catcgccaat
    17941 cccgcgctcg acaaaggtgt tagtggagcc gcgcgcgcca gaatccagaa cttcgacgtt
    18001 tttatacagt gcccgaacaa aatcctgtgc ttttgcctga tcgttgttgt tgtgatgcag
    18061 cgcgtagccc caggcagcta ggtagttcca gcgtgcgcca ccagagcttt tcggattagg
    18121 cgtgatcacc gaaacacccg gtttaatcag atcgttccag tcatggatct gcttcggatt
    18181 gcccttacgc accaggaaaa caatggtgga agtgtacggt gcggagttat ccggcagacg
    18241 tttgatccac tctttatcaa tccgcccacg ttccgcaatc gcgtccacgt cataggccag
    18301 agccagcgtg acaacatcag cttcaatacc gttgattacc gacgtcgctt gtttacctga
    18361 accaccgtgt gactgacgaa tcaccacgtt atcaccagtt tgctgtttcc agtgggcgct
    18421 gaatgccttg ttgtactgtt cgtacaattc gcgcgttgga tcatatgaaa cgttaagaag
    18481 ctgaatatcc tttgccataa cgctggttgc cgccagcaaa aatgttaacc ctacgcccca
    18541 cttgttcatc gcccgactct cttatgttgt gttgtgatga gcaaagcgtg ccagaaggtt
    18601 aaccaaacat taaagaataa aaaaagattg gctataactt gcgggtatat gttgagggat
    18661 taaaaaggcg gaagaaatcc gcctcatatt gctgacaaag tgcgctttgt ccatgccgga
    18721 tgcggcgcga acgccttatc cggcctacaa aagtttgcaa attcaataaa ttgcagaatt
    18781 catgtaggcc tgataagcga agcgcatcag gcatttttgc ttctgtcatc ggtttcaggc
    18841 taaaggaatc tgcctttttc cgaaatcatt aatacagttt tttcgcgcag tccagccagt
    18901 cacctttgaa cggacgcttc atgttttcga tagcgtcgat gatgtcgtgg tgaaccagct
    18961 gttcgttctg gatacctaca caacgaccgc cgtaacctgc cagcagcaga tcgatagcgt
    19021 aagcgcccat acgggaagcc agaatacggt cgtaaggcac cggagaacca ccgcgctgga
    19081 tgtggcccag cacagttgcg cgggtttcac gaccggtttc tttctcgatg aaatgcgcca
    19141 gttcgtcaac atcacacata tgttcggtaa tcgccacgat cgcgtgtttt ttacctttcg
    19201 cgatacccgc tttgatttcg tttaccaggt cttcacggct gaattcaact tccggaacca
    19261 caacgaattc acagccaccg gcaatggccg cagccaacgt cagatcgcca caataacggc
    19321 ccatcacttc caccacggaa atacgctggt gagaagaaga ggtgtcacgc agacggtcga
    19381 tcgcttctac aacggtgctc agcgcagtga agaaaccgat agtgtagtca gtgcctttga
    19441 tgtcgttgtc gatagtgccc ggcagaccga tgcacgggaa gcccatttcg gtcagacgca
    19501 ttgcacccat gtaggaaccg tcaccgccga taaccaccag cgcgtcgata ccacgttttt
    19561 tcaggttttc gatagccacg gcgcggatgt tctcgtcgcg gaattccggg aagcgcgcag
    19621 aaccgaggaa cgtaccgcca cggttgatca tgtcagaaac gctgtaacgg tctagctgta
    19681 ccatacggtc ttcatacaga cccagatagc cgtcataaat gcccattact tccagacctt
    19741 ctgtcagcgc agaacgaaca accccgcgaa ttgcggcgtt catgcctggc gcatcaccgc
    19801 cgcttgtcaa cacaccgatt ttcttaatca tgactacctc tgaactttgg aatgcaaaat
    19861 aaaatctgtt gccggaagtc ttcttgcaca tcgaagtgat ccaacgaatg tgcaaatagt
    19921 ataacaatca cttcctgctg aattgattca ggtcaggcca aatggcggta ttttatacac
    19981 aaaatgcggg tctggctctc ttttatactg attatgaaag catagaccgt ttaccctccc
    20041 tgggtacgac ggaacagggg tcctgatgga taattacatc cgatcccgga aaacgccgta
    20101 aaatagcctg ctctacctga tccgccacca tatgtgcctg aaccaaaggc agagagtctt
    20161 ccatttccaa atgaatctga ataaagcggg tcggccctga ctgccgcgtg cgaagatcgt
    20221 gagcgccgct aacacccggc caggaagtca cgatatcaat aatttcttgc cgttcctcat
    20281 caggcaatgc gcgatccagt aatgactgta ccgcctcata tcccatgcgt aacgcgctat
    20341 ataaaatata gatgccgatt cccaatgcaa acagagcatc ggcgcgatgc cagccgtacc
    20401 aggacaaccc cagcgccagc agaattgcgc cgttcatcat aacatcagac tggtaatgta
    20461 gcatatcagc ccgcaccgcc tggctttgcg tccggcgcac cacccaccgc tgaaacgaga
    20521 caaggataat cgtacaaatt agcgccacaa ttgtcacgat aaccccgacg cctggatctg
    20581 tcatcggtgt tggagatacc agatgttgaa tacccgtcaa aaacaggaat agtgccgaac
    20641 cggagataaa catactttgc gccagcgccg cgagggactc agctttaccg tgaccaaacg
    20701 agtgattatc gtcggcaggt tgcagggaat atcgcaccac cagtaaattc gtcaacgacg
    20761 cgccgatatc caccagcgaa tccaccagcg cggcgagaat actcaccgac ccggtatacc
    20821 accatgcaaa aattttaatc agcaatagca gcgaagccat cgccgtcgca gcaatcgccg
    20881 cccgactgac cagccgtcca taagattgat tcataaatac tcccactatc aactgacgct
    20941 agtataacgg aagcaaatca tctgcaatgc attaagcagc aggcaaattg aggataaaaa
    21001 aaaccccaca tcatgtgggg gaagacaggg atggtgtcta tggcaaggaa aacagggttt
    21061 actactggga acgtgagttg ctactactca atagcttcaa cgatgaactt ttttgccatt
    21121 gcgtcacgtc acgcaactgc tccattcgtt gttgatgttt ctcgtttaaa accgcttgct
    21181 gctccggcgt taacaggcga tacatttggt tgcggacttt tgccatctca acctgacgag
    21241 caatttgctc attcgccatt ttttctgcct gtgcgcgcac agcgttttca tcaaaatttt
    21301 ctgcggtgac aaggcgatgc attgtctcca gttcgctaac attaacagga ggctgttcgt
    21361 gccgggcctg ttgcataaga tctcgcatct gctgacgctg atgttcggtt aaacttatgc
    21421 cgtcgaacat atggctctgc gtactgcgct gcgtaagttc ttcacccgga tgccagttat
    21481 cgcctgaacc gacttcagca gcgtggctta atgaactgac tgccagcgtt gaggccatga
    21541 cggcagcggt aactatgcgc atcatttgct cccaaaatct ttctgtcgcg attcaacgat
    21601 agagagttta cgattcaggc tgcaaacatg cgtcaggggg tgtaaaacaa cgtaaagtca
    21661 tggattagcg acgtctgatg acgtaatttc tgcctcggag gtatttaaac aatgaataaa
    21721 atcctgttag ttgatgatga ccgagagctg acttccctat taaaggagct gctcgagatg
    21781 gaaggcttca acgtgattgt tgcccacgat ggggaacagg cgcttgatct tctggacgac
    21841 agcattgatt tacttttgct tgacgtaatg atgccgaaga aaaatggtat cgacacatta
    21901 aaagcacttc gccagacaca ccagacgcct gtcattatgt tgacggcgcg cggcagtgaa
    21961 cttgatcgcg ttctcggcct tgagctgggc gcagatgact atctcccgaa accgtttaat
    22021 gatcgtgagc tggtggcacg tattcgcgcg atcctgcgcc gttcgcactg gagcgagcaa
    22081 cagcaaaaca acgacaacgg ttcaccgaca ctggaagttg atgccttagt gctgaatcca
    22141 ggccgtcagg aagccagctt cgacgggcaa acgctggagt taaccggtac tgagtttacc
    22201 ctgctctatt tgctggcaca gcatctgggt caggtggttt cccgtgaaca tttaagccag
    22261 gaagtgttgg gcaaacgcct gacgcctttc gaccgcgcta ttgatatgca catttccaac
    22321 ctgcgtcgta aactgccgga tcgtaaagat ggtcacccgt ggtttaaaac cttgcgtggt
    22381 cgcggctatc tgatggtttc tgcttcatga taggcagctt aaccgcgcgc atcttcgcca
    22441 tcttctggct gacgctggcg ctggtgttga tgttggtttt gatgttaccc aagctcgatt
    22501 cacgccagat gaccgagctt ctggatagcg aacagcgtca ggggctgatg attgagcagc
    22561 atgtcgaagc ggagctggcg aacgatccgc ccaacgattt aatgtggtgg cggcgtctgt
    22621 tccgggcgat tgataagtgg gcaccgccag gacagcgttt gttattggtg accaccgaag
    22681 gccgcgtgat cggcgctgaa cgcagcgaaa tgcagatcat tcgtaacttt attggtcagg
    22741 ccgataacgc cgatcatccg cagaagaaaa agtatggccg cgtggaactg gtcggtccgt
    22801 tctccgtgcg tgatggcgaa gataattacc aactttatct gattcgtccg gccagcagtt
    22861 ctcaatccga tttcattaac ttactgtttg accgcccgct attactgctg attgtcacca
    22921 tgttggtcag tacgccgctg ctgttgtggt tggcctggag tctggcaaaa ccggcgcgta
    22981 agctgaaaaa cgctgccgat gaagttgccc agggaaactt acgccagcac ccggaactgg
    23041 aagcggggcc acaggaattc cttgccgcag gtgccagttt taaccagatg gtcaccgcgc
    23101 tggagcgcat gatgacctct cagcagcgtc tgctttctga tatctctcac gagctgcgca
    23161 ccccgctgac gcgtctgcaa ctgggtacgg cgttactgcg ccgtcgtagc ggtgaaagca
    23221 aggaactcga gcgtattgaa accgaagcgc aacgtctgga cagcatgatc aacgatctgt
    23281 tggtgatgtc acgtaatcag caaaaaaacg cgctggttag cgaaaccatc aaagccaacc
    23341 agttgtggag tgaagtgctg gataacgcgg cgttcgaagc cgagcaaatg ggcaagtcgt
    23401 tgacagttaa cttcccgcct gggccgtggc cgctgtacgg caatccgaac gccctggaaa
    23461 gtgcgctgga aaacattgtt cgtaatgctc tgcgttattc ccatacgaag attgaagtgg
    23521 gctttgcggt agataaagac ggtatcacca ttacggtgga cgacgatggt cctggcgtta
    23581 gcccggaaga tcgcgaacag attttccgtc cgttctatcg gaccgatgaa gcacgcgatc
    23641 gtgaatctgg cggtacagga ttggggctgg cgattgttga aaccgccatt cagcagcatc
    23701 gtggctgggt gaaggcagaa gacagcccgc tgggcggttt acggctggtg atttggttgc
    23761 cgctgtataa gcggagttaa actccgcatt tgtaggcagg ataaggcgtt tacgccgcat
    23821 ccggcatttg agcaggatgc ctgatgcgac gctgatagcg tcttatcagg cctacactcc
    23881 gggggttatt ttccccacaa ccggcgggaa aaatcttcga tcttgccgct cagtcggcgc
    23941 ttttgcatcg tccgcgtcca gcttttcgat aacccggcgg cggagagtaa acggtgatac
    24001 tgatcgtcat caaacggcat atgccatgcg atggcggcag cctcctgcac ggtcacatcg
    24061 ctgacacggg aaaccaactc cagcggcgcg tccgcagaca ctttccctgg cgctatcacg
    24121 ctatacaacc accccacctt acctgtgttt tgcatcaact gcgctatatc gctgatatca
    24181 aaatggtaat tgagtttata gcaaggcgag cgcggctggc tgacctgaat taatgcctct
    24241 ccccagcgga aaatatcgcc catatgaaca ttactttccg tgagaccgtc ggttgagaga
    24301 ttttcgccaa acgcaggcgc aacaaacaac tccgcctgtt ccggaaattc ccgcacccag
    24361 tagagatagt gctcacgagg ataatgacac agcgctctgt ctggcccgcc gtgaaccttt
    24421 ttctccgcct gctcgtcacc ttccagcccc agctccgtca gcatcaactc accatcaacc
    24481 tggattttgg caattgcgct gggtttgcct tcgggataag cctgaatctt gcctgtgtat
    24541 acatcaaccg gatatcgcat ctgttccctc tccacgagaa atttccccat tagaccatat
    24601 ttattttatg tcaggcgtgc tgttttcgcg gtaagtatcc aggcaggaac tcaatagcaa
    24661 accggacgaa cgttagttca tccggctgtg gggattaagg aagcaggttt atttcaccgt
    24721 acctaaaatt tcgattttcg ctgctctggc tttgacttta cgtgaccaga tagaggtaag
    24781 aattggcacc agaatcgaag tcacaatgac cgccgtcgct accagcgaag ttgctgccgg
    24841 agccatcggt ttaaacgcag gcaccatttc tgcaatcagc acaggtgtcg ctaccgcggc
    24901 ccctgcggaa ctggaagcgg caattccggc agtgccatcg ccaccgccaa tcaatttatc
    24961 ggcgataatc aacggaatac cggtcacgat aattactgcc acacccaaca ggatccccag
    25021 caaaccagtc tgggcaatca cagtcaaatc aatggtattg cccagcgcga aggcaaagaa
    25081 tggaatcagc gtttgcactg ctttgctgaa aaattctcgt aattcagggt caagattccc
    25141 cagggcaaag cccaccagga acggtaatac tgcgccgacg aaaacatgcg gttcaaacga
    25201 ggcaatcccg gcagtgccca gaataatcat cgtcatgagc ggcccggact ccaacgacat
    25261 caacacaaat gccccagctt cttctttcgt accgtactgc tgcatgatgg aagcgtaaag
    25321 tccgccgttg gtcatatcca tcgccgccac cagcgccagc gttgaaagtc cggcaaagaa
    25381 tccaacttca acaccatgtt ccggaataat gcgcgaggca attgccgcaa ccacccacgc
    25441 gacggcaatt ttagttacca ccagcgtacc ggatttacgc agtaccgttc ccgtcgcgct
    25501 taattttatt gacgccccca tgcaaaaaaa ccacaccgcc agaatgggca ccgtaccggt
    25561 aatcatcccg ttggtgaatg atccaaaata tttccccgcc cccggcgaga aggtgtggca
    25621 cagtgcgcca aggaatagcg ggacgagcat catccccccc gggattttct caatcgagcg
    25681 ttttatctgc atttccatca cctgtcggca tatttttaag ggctgatgcg taattcctca
    25741 atggagcatc agtgttgatg gaaacgaaga tagacattca tttaatcaat aaaagtgatc
    25801 aaaaccacat caattgaaac gctgttttat ttttatcgga tcattgtttt tattttgaaa
    25861 ttgatcacaa aaaaaccacc gccgttggcg atggttcatt ttttaagctg atatgcggcc
    25921 tatacgcctc attgcagcag gcggcaaatg attatttttt cgccgcaaaa cgtgccgctg
    25981 cttcgtccca gttcaccacg ttccagaact ctttaatgta gtccggacgg cggttctgga
    26041 atttcaggta gtaagcatgt tcccacacat ccaggcccag aatcgggaag ccggaagcgc
    26101 cagaaatagc ttcacccatc agcggagaat cctggttagc agtagaaacc accgccagtt
    26161 tatcgccttt cagcaccagc catgcccagc cggaaccaaa gcgggaagct gccgcttttt
    26221 caaattctgc tttgaagtta tcaacggagc cgaagtcacg ttcgatagcc gctttcaggt
    26281 caccctgcag ggtggtgcct tttttcagac ctttccagaa caggctgtgg ttagcgtgac
    26341 cgccagcgtt gttgcgcagt acggttttct tgtctgctgg cagctggtcc agtttggtga
    26401 tcagctcttc aaccggcagg ttggcaaatt ctggcaggct ttccagcgcc gcgttggcgt
    26461 tgtttacgta ggtctgatgg tgtttggtgt ggtggatttc catggtctgc ttatcgaagt
    26521 gcggttccag ggcatcgtaa gcatacggca gggatggcag ggtatagctc atattcatct
    26581 ccagtattgt cgggcggccg attgttaatg ccgcgtaagc agttggttca ttatagttaa
    26641 ttaaatgata ttgaaaatga ttatcaatgc cgtacttttc gtaagggtat ggttttgcag
    26701 gaaaatgccc gagatgtgaa gcaaatcacc cacttaatgc cgtgattgcc agtaaatcga
    26761 caacggcggc aacaggcgaa aggttaatcg acagcacgat ttttacactc atctcgtcgg
    26821 agatgtgaag cgacgaaaaa tgatgaggat aagaagatga gtaacgcgat tacgatgggg
    26881 atattttggc atttgatcgg cgcggccagt gcagcctgtt tttacgctcc gttcaaaaaa
    26941 gtaaaaaaat ggtcatggga aaccatgtgg tcagtcggtg ggattgtttc gtggattatt
    27001 ctgccgtgga ccatcagcgc cctgttacta ccggatttct gggcgtatta cagctcgttt
    27061 agtctctcta cgctgctgcc tgtttttctg ttcggcgcta tgtgggggat cggtaatatc
    27121 aactatggcc tgaccatgcg ttatctcggc atgtcgatgg gaattggcat cgccattggc
    27181 attacgttga ttgtcggtac gctgatgacg ccaattatca acggcaattt cgatgtgctg
    27241 attaacaccg aaggcggacg catgacgttg ctcggcgttc tggtggcgct gattggcgta
    27301 gggattgtaa ctcgagccgg gcagttgaaa gagcgcaaga tgggcattaa agccgaagag
    27361 ttcaatctga aaaaagggct ggtgctggcg gtgatgtgcg gcattttctc tgccgggatg
    27421 tcctttgcga tgaacgccgc aaaaccgatg catgaagccg ctgccgcact tggcgtcgat
    27481 ccactgtatg tcgctctgcc aagctatgtt gtcatcatgg gcggcggcgc gatcattaac
    27541 ctcggtttct gttttattcg tctggcaaaa gtgaaggatt tgtcgctaaa agccgacttc
    27601 tcgctggcaa aaccgctgat cattcacaat gtgttactct cggcactggg cgggttgatg
    27661 tggtatctgc aattcttttt ctatgcctgg ggccacgccc gcattccggc gcagtatgac
    27721 tacatcagtt ggatgctgca tatgagtttc tatgtattgt gcggcggtat cgtcgggctg
    27781 gtgctgaaag agtggaacaa tacaggacgc cgtccggtaa cggtgttgag cctcggttgt
    27841 gtggtgatta ttgtcgccgc taacatcgtc ggcatcggca tggcgaatta atctttctgc
    27901 gaattgagat gacgccactg gctgggcgtc atcccggttt cccgggtaaa caccaccgaa
    27961 aaatagttac tatcttcaaa gccacattcg gtcgaaatat cactgattaa caggcggcta
    28021 tgctggagaa gatattgcgc atgacacact ctgacctgtc gcagatattg attgatggtc
    28081 attccagtct gctggcgaaa ttgctgacgc aaaacgcgct cactgcacga tgcctcatca
    28141 caaaatttat ccagcgcaaa gggacttttc aggctagccg ccaaccgggt aatcagctta
    28201 tccagcaacg tttcgctgga tgttggcggc aacgaatcac tggtgtaacg atggcgattc
    28261 agcaacatca ccaactgccc gaacagcaac tcagccattt cgttagcaaa cgacacatgc
    28321 tgactacttt catgctcaag ctgaccgata acctgccgcg cctgcgccat ccccacgcta
    28381 cctaagcgcc agtgtggttg ccctgcgctg gcgctaaatc ccggaatcgc tccctgtcag
    28441 tcaagattca gcttcagccg ctccgggcaa taaataatat tctgcaaaac cagatcgtta
    28501 acggaagcgt aggagtgttt atcgtcagca tgaatataaa agagatcgcc acgggtaatg
    28561 cgataaggac gatcgttgag tacatgcaga ccattaccgc gccagacaat caccagctca
    28621 caaaaatcat gtgtatgttc ggcaaagaca tcttgcggat aacggtcagc cacagcgact
    28681 gcctgctggt cgctggcaaa aaaatcatct ttgagaagtt ttaactgatg cgccaccgtg
    28741 gctacctcgg ccagagaacg aagttgatta ttcgcaatat ggcgtacaaa tacgttgaga
    28801 agattcgcgt tattgcagaa agccatcccg tccctggcga atatcacgcg gtgaccagtt
    28861 aaactctcgg cgaaaaagcg tcgaaaagtg gttactgtcg ctgaatccac agcgataggc
    28921 gatgtcagta acgctggcct cgctgtggcg tagcagatgt cgggctttca tcagtcgcag
    28981 gcggttcagg tatcgctgag gcgtcagtcc cgtttgctgc ttaagctgcc gatgtagcgt
    29041 acgcagtgaa agagaaaatt gatccgccac ggcatcccaa ttcacctcat cggcaaaatg
    29101 gtcctccagc caggccagaa gcaagttgag acgtgatgcg ctgttttcca ggttctcctg
    29161 caaactgctt ttacgcagca agagcagtaa ttgcataaac aagatctcgc gactggcggt
    29221 cgagggtaaa tcattttccc cttcctgctg ttccatctgt gcaaccagct gtcgcacctg
    29281 ctgcaatacg ctgtggttaa cgcgccagtg agacggatac tgcccatcca gctcttgtgg
    29341 cagcaactga ttcagcccgg cgagaaactg aaatcgatcc ggcgagcgat acagcacatt
    29401 ggtcagacac agattatcgg tatgttcata cagatgccga tcatgagcgc gtacgaaaca
    29461 gaccgtgcca ccggtgatgg tatagggctg cccattaaac acatgaatac ccgtgccatg
    29521 ttcgacaatc acaatttcat gaaaatcatg atgatgttca ggaaaatccg cctgcgggag
    29581 ccggggttct atcgccacgg acgcgttacc agacggaaaa aaatccacac tatgtaatac
    29641 ggtcatactg gcctcctgat gtcgtcaaca cggcgaaata gtaatcacga ggtcaggttc
    29701 ttaccttaaa ttttcgacgg aaaaccacgt aaaaaacgtc gatttttcaa gatacagcgt
    29761 gaattttcag gaaatgcggt gagcatcaca tcaccacaat gaagctaatt gtgaacatca
    29821 tcacgttcat ctttccctgg ttgccaatgg cccattttcc tgtcagtaac gagaaggtca
    29881 cgaattcagg cgctttttag actggtcgta atgaaattca gcaggatcac attatgacct
    29941 ttcgcaattg tgtcgccgtc gatctcggcg catccagtgg gcgcgtgatg ctggcgcgtt
    30001 acgagcgtga atgccgcagc ctgacgctgc gcgaaatcca tcgttttaac aatggcctgc
    30061 atagccagaa cggttatgtc acctggaatg tggatagcct ggaaagtgcc attcgccttg
    30121 gattaaacaa ggtgtgcgag gaagggattc gtatcgatag cattgggatt gatacctggg
    30181 gcgtggactt tgtgctgctc gaccaacagg gtcagcgtgt gggcctgccc gttgcttatc
    30241 gcgatagccg ctccaatggc ctaatggcgc aggcacagca acaactcggc aaacgcgata
    30301 tttatcaacg tagcggcatc cagtttctgc ccttcaatac gctttatcaa ctgcgtgcgc
    30361 tgacggagca acaacctg
//
LOCUS       DAJVQG010000033        30001 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJVQG010000033 DAJVQG010000000
VERSION     DAJVQG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30001
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.033"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            38..580
                     /locus_tag="OQM83_001369"
     CDS             38..580
                     /locus_tag="OQM83_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1519665.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            663..1364
                     /locus_tag="OQM83_001370"
     CDS             663..1364
                     /locus_tag="OQM83_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS1519666.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1389..3988
                     /locus_tag="OQM83_001371"
                     /pseudo
     CDS             1389..3988
                     /locus_tag="OQM83_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3979..5049
                     /locus_tag="OQM83_001372"
     CDS             3979..5049
                     /locus_tag="OQM83_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1519667.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5061..5603
                     /locus_tag="OQM83_001373"
     CDS             5061..5603
                     /locus_tag="OQM83_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1519668.1"
                     /translation="MKKETIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5611..6126
                     /locus_tag="OQM83_001374"
     CDS             5611..6126
                     /locus_tag="OQM83_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1519669.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6092..6829
                     /locus_tag="OQM83_001375"
     CDS             6092..6829
                     /locus_tag="OQM83_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCS1519670.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6940..7950
                     /gene="pyrD"
                     /locus_tag="OQM83_001376"
     CDS             6940..7950
                     /gene="pyrD"
                     /locus_tag="OQM83_001376"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCS1519671.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8124..8666
                     /gene="zapC"
                     /locus_tag="OQM83_001377"
     CDS             8124..8666
                     /gene="zapC"
                     /locus_tag="OQM83_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCS1519672.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="OQM83_001378"
     CDS             complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="OQM83_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCS1519673.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVMPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10016..12124
                     /gene="rlmKL"
                     /locus_tag="OQM83_001379"
     CDS             10016..12124
                     /gene="rlmKL"
                     /locus_tag="OQM83_001379"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCS1519674.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12136..14043
                     /locus_tag="OQM83_001380"
     CDS             12136..14043
                     /locus_tag="OQM83_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1519675.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQEPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLDQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQMADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14173..15426
                     /gene="pqiA"
                     /locus_tag="OQM83_001381"
     CDS             14173..15426
                     /gene="pqiA"
                     /locus_tag="OQM83_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCS1519676.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSQQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15431..17071
                     /gene="pqiB"
                     /locus_tag="OQM83_001382"
     CDS             15431..17071
                     /gene="pqiB"
                     /locus_tag="OQM83_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCS1519677.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17068..17631
                     /gene="pqiC"
                     /locus_tag="OQM83_001383"
     CDS             17068..17631
                     /gene="pqiC"
                     /locus_tag="OQM83_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCS1519678.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17887..18054
                     /gene="rmf"
                     /locus_tag="OQM83_001384"
     CDS             17887..18054
                     /gene="rmf"
                     /locus_tag="OQM83_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCS1519679.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="OQM83_001385"
     CDS             complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="OQM83_001385"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCS1519680.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18711..20471)
                     /locus_tag="OQM83_001386"
     CDS             complement(18711..20471)
                     /locus_tag="OQM83_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCS1519681.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20657..21109
                     /gene="matP"
                     /locus_tag="OQM83_001387"
     CDS             20657..21109
                     /gene="matP"
                     /locus_tag="OQM83_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCS1519682.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="OQM83_001388"
     CDS             complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="OQM83_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCS1519683.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="OQM83_001389"
     CDS             complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="OQM83_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCS1519684.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23308..23937
                     /gene="sxy"
                     /locus_tag="OQM83_001390"
     CDS             23308..23937
                     /gene="sxy"
                     /locus_tag="OQM83_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCS1519685.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="OQM83_001391"
     CDS             complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="OQM83_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCS1519686.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26072..26518)
                     /locus_tag="OQM83_001392"
     CDS             complement(26072..26518)
                     /locus_tag="OQM83_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCS1519687.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26641..28695
                     /gene="helD"
                     /locus_tag="OQM83_001393"
     CDS             26641..28695
                     /gene="helD"
                     /locus_tag="OQM83_001393"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCS1519688.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISSQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="OQM83_001394"
     CDS             complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="OQM83_001394"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCS1519689.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29281..29943)
                     /locus_tag="OQM83_001395"
     CDS             complement(29281..29943)
                     /locus_tag="OQM83_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCS1519690.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
BASE COUNT         7732 a         7084 c         7879 g         7306 t
ORIGIN      
        1 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
       61 gtttataact gtggtatgtg caacgtccag cgttatggca gctgatgata atgctatcac
      121 ggatggcaaa gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      181 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      241 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      301 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      361 tgccaaccag gctaccaccg atgcagcgac gaatgttgct ctacaaatgt atctgccaga
      421 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cggattctgc
      481 tgagcaaaca gtgaccttta aagttgatta tatcgctacg gggaaagcga cttctggtaa
      541 tgtgaatgcg gtaacaaatt tccatattaa ctattattaa tagaactcat taattgtttt
      601 attaattagt acccctctag tgttctggag gggatattca tattttttaa gagtgactat
      661 ttatgaaaac ttgcataaca aagggaattg tgaccgtaag tttaacggca atattactca
      721 gctgctcatc cgcatgggcg gcaggtaaag gcgggattgg acttgcagca acacgtcttg
      781 tttattcaga aggtgaagag caaatttcac tgggtgtacg taacaccagt ccggatgttc
      841 cttatcttat tcagtcatgg gtgatgaccc cagataataa aaaatcagca gattttatta
      901 ttacaccacc gctatttgtg ctgaatccgg ctaatgagaa tctgttacgc attatgtaca
      961 ttggtgcgcc gttggcgaaa gacagagaaa cccttttctt cactagcgta cgggcagtcc
     1021 cttcaacaac gaagcgggaa gagggaaata ccctgaagat tgccacacaa agcgtcatca
     1081 aacttttctg gcgaccaaaa ggtttagcgt atcccttagg cgaggctccg gcgaaactgc
     1141 gttgcacttc gtcagctgac atggttacgg tcagtaaccc aacaccttat ttcattaccc
     1201 tgacagacct gaaaataggg ggaaaggtag ttaaaaatca aatgatttcc ccctttgata
     1261 aataccaatt ctctctgcca aagggggcca aaaatagcag cgtaacgtat cgaaccatca
     1321 atgattacgg ggcggaaacg ccacaactca actgtaaatc gtaagccgtc ttcagttaag
     1381 agagcgagat gtatagaact catcgacaac acagcctgtt aagctctggt ggagtgccat
     1441 cgtttattgg tgggctggtg gtgtttgtgt cggcagcgtt caatgcacaa gctgaaacct
     1501 ggttcgatcc tgcttttttc aaagatgatc cctcaatggt ggccgatttg tctcgtttcg
     1561 aaaaaggaca aaaaataacg ccaggggttt atcgtgtcga tattgttctg aatcagacaa
     1621 ttgtagatac gcgcaacgtc aattttgttg agataacgcc agagaagggg attgccgcct
     1681 gtttgacgac tgaaagcctg gatgcaatgg gtgttaatac tgatgcgttt ccggctttta
     1741 aacaactgga caaacaagcg tgtgtgccat tggcggagat tattccggat gccagcgtaa
     1801 cgtttaatgt gaataaactc cgtctggaaa tttcagtacc gcaaatcgcc atcaaaagta
     1861 atgcccgtgg ttatgtcccc cctgaacgtt gggatgaagg gatcaacgcg ctattactgg
     1921 gatattcgtt tagcggggct aacagtattc atagcagcgc agacagtgat tctggcgaca
     1981 gctattttct gaatttaaac agtggcgtta atttaggccc atggagattg cgcaacaatt
     2041 caacatggag tcgcagtagt ggccaaaccg cagaatggaa gaatctcagc agctatttgc
     2101 agcgggcggt tattccacta aaaggcgaac tgaccgtagg tgatgattat actgcaggcg
     2161 attttttcga tagtgtcagc tttcgtggtg tgcagctggc gtcagatgac aacatgctgc
     2221 cagacagcct gaaagggttt gcgcctgtgg tgcgtggtat cgccaaaagc aatgcccaga
     2281 taacgattaa gcaaaatggt tacaccattt accaaactta tgtatcgcct ggtgcttttg
     2341 aaattagtga tctctattcc acgtcgtcga gcggtgattt gttagttgaa atcaaagaag
     2401 cggacggcag cgtcaatagc tacagcgtac cgttttccag cgtgccatta ctccagcgtc
     2461 aggggcgaat caaatacgcg gtgacactgg cgaaatacag aaccaatagt aatgaacagc
     2521 aggagagcaa atttgcctag gccacgttgc agtggggcgg accgtgggga acgacatggt
     2581 atggtggtgg acaatatgct gaatattacc gtgccgccat gtttggtctg ggatttaacc
     2641 ttggcgattt cggagcaatt tcgttcgatg cgacccaggc gaagagtacg ctggcagacc
     2701 aaagcgaaca taaaggtcag tcatatcgtt ttctgtatgc caaaacgctc aaccaattgg
     2761 gcactaattt tcaattgatg ggctatcgct attcgacgtc gggtttctac accctttccg
     2821 acaccatgta taaacatatg gatggctacg aatttaatga cggtgatgat gaagatacgc
     2881 cgatgtggtc gcgttattac aatttgtttt acaccaaacg tggcaaactg caggtcaata
     2941 tctcccagca attaggcgag tacggttcgt tttatttaag tggtagccag caaacttact
     3001 ggcataccga tcaacaggat cggctattac agtttggcta caacacgcaa attaaagatc
     3061 tctcgctggg gggttcctgg aactacagta agtcccgtgg tcaacctgat gctgatcagg
     3121 tgtttgcact aaatttttcc ctgccgctca atctgttgct ccccagaagt aatgatagct
     3181 ataccaggaa aaaaattacg cctggatgac ctctaacacc agcatcgata acgaagggca
     3241 cattacacaa aacctgggtt taacggagac actactcgat gacggtaacc tgagctacag
     3301 cgtgcaacag ggatataaca gcgaggggaa aacggctaat ggtagcgcca gcatggacta
     3361 caaaggggcg tttgcagatg cccgagtggg ctacaactac agcgataacg gcagtcaaca
     3421 acaactgaac tacgctcttt caggcagttt agttgcccat tcacagggca ttaccctggg
     3481 gcaatcgctg ggggaaacta acgtgctgat tgcagcacca ggcgcagaga atactcgtgt
     3541 ggcgaacagc accgggctga aaactgactg gcgcggatat accgttgtcc cctatgccac
     3601 ttcttatcgg gaaaatcgaa tcgcacttga tgcggcatcg ttaaaacgta acgtggatct
     3661 tgaaaatgca gtagtcaacg tggttcccac caaaggggcg ttggttctgg cggagttcaa
     3721 tgcccatgcg ggagcaaggg tattaatgaa aacatcaaag cagggtatac cgctgcgttt
     3781 tggcgcgata gcgacgctgg acggcgtaca gactaatagc ggcattattg atgatgatgg
     3841 ctcgctctat atgtccggtt tgcccgctca gggagcgata actgtacgct ggggcgaagc
     3901 tcccgatcaa atttgtcata tcagttacca gcttaccgaa caacaaatta actctgcgat
     3961 tacgcgaatg gatgccatat gcagataatc tttggagaaa aatgcgtgtt attactacga
     4021 ctattttttg ccgccgtctt aatgctatgg tgcgctcaaa ctgctgctta tagcgggcag
     4081 tgtcatacca ctcaggggaa tccgtatatt ggcgtcaatt ttggcgttaa aaccctggag
     4141 gaagaagaaa atacggctgg agtagttaaa gacaaatttt atcagtggaa cgaatcgaat
     4201 gattattatg tttcctgtga ttgcgataaa gacaatgtca gaagtggccg atgggcattc
     4261 gccgcggatt caccgttagt ctatttaggc gacaactggt acaaaattaa tgactatctt
     4321 gccgccaaag ttttattgca ggttaaaggc agttctccta ctgcggttcc tttcgaaaac
     4381 gtgggcacag gggcagatac acgatggcat atttgcgatc ccggcggtca acgtttaggt
     4441 ggccaggggg ctagcggtaa tagcggtagc ttttccctga aaatattgca gccgttcgtt
     4501 ggttcggtcg tcattcctcc tatggcgctg gcgcgattat ttgaatgcta caacataccc
     4561 gcaggtgatt cctgcacgac tacaggtaca ccggttttag tgtattacct gtctggtacg
     4621 atcaattcac ttggctcatg ttccgtcaat gccggagaaa caatcgaggt cgatctgggc
     4681 gacgcatttg cggctaactt tcgtgttgta gggcataagc ctcttggggc cagaacggca
     4741 gaacttgcaa ttccagtcag gtgtaacacg ggaaacgcgg ggttagttaa cgtcaacctg
     4801 agtctgacgg caaccacaga ccccagctat ccccaggcga ttaagacgtc acgtcctggc
     4861 gtgggcgtgg tggtgaccga tagccagaac aacattattt cccctgctgg tggaacatta
     4921 ccgctctcta ttcctgatga tgcagacagt atcgcgcgaa tgaatgtcta tccagtcagc
     4981 acgacaggtg taccaccaga aaccgggcga tttgaagcca cggcaacggt gagaataaat
     5041 tttgattaac gcgtgaacgt atgaagaaag aaacgatatt tcagtgcgtt atcttgttct
     5101 ttagcattct taacatccat gtcgggatgg ctgggcctga acaagttagt atgcatattt
     5161 atgggaatgt ggtcgatcag ggctgtgatg tcgccaccaa aagtgcatta caaaatattc
     5221 atattggtga ttttaatatc agtgattttc aggccgcgaa taccgtaagc actgctgctg
     5281 atttgaatat cgatatcacc ggttgtgccg ctagtattac tggcgcggac gtccttttta
     5341 gcggcgaggc tgacaccctt acgccgacac tgctcaaact aactgacaca ggcggaagcg
     5401 gtggtatggc aacggggatt gccgtgcaaa ttcttgatgc gcaaagtcag caagaaatcc
     5461 cgctcaatca ggtccagcct cttacgccct taaaagccgg ggataacaca ctcaaatatc
     5521 aacttcgtta taagtccaca aaggcgggag caacgggcgg taatgcgacg gcggttctct
     5581 attttgatct ggtttaccag tgaaggagtg atgttgaaac ggataatctg gattctgttc
     5641 ttactgggat taacgggggg ctgtgagcta tttgcccatg atggcacggt caacattagc
     5701 ggatcgtttc gccgtaatac atgcgtgctg gcacaggata gcaagcaaat caatgtgcag
     5761 ttaggcgatg tctcgctgac acgttttttt catgggaatt atggcccgga gaaatctttc
     5821 atcattaatt tgcaggattg cggaacagat gtcagcacgg ttgacgtcac cttttcagga
     5881 acccccgacg gagtgcagag cgaaatgctg tccattgaaa gtggtacgga tgcagccagc
     5941 gggctggcaa ttgcgattct ggatgatgcg aagatattga ttccgcttaa ccaggcgagt
     6001 aaagactaca gcctacatag cggtaaggtg ccattaactt tttatgcgca attgcgacct
     6061 gtcaatagcg atgtgcagtc gggtaaagtg aatgccagcg cgacatttgt acttcattat
     6121 gactaatacg tggaacagat tggcgctctt gattttcgcc gttttatcgc tgctggtggc
     6181 gggtgaattg cgggcaggag tggtggtcgg cggaacgcga tttatctttc cggcagacag
     6241 agaatcgata tctattttac tgactaatac cagtcaggaa tcctggctta ttaacagtaa
     6301 aatcaaccgc ccaacgcgtt gggccggggg ggaagcgtcg acagtgccag caccattact
     6361 ggccgctccg ccacttattc tcctgaagcc cggtactaca ggcacgttgc gcttgctgag
     6421 aacggaaagc gacatcttgc ctgtggatcg cgaaacgcta tttgagttaa gcattgccag
     6481 cgtgccatcc ggcaaagttg aaaatcagag cgtaaaagtg gcgatgcgct cggtatttaa
     6541 actgttctgg cgacccgaag gtttgccggg cgacccgctg gaagcttatc aacaattacg
     6601 ctggacacgg aattcgcagg gtgtacaact cactaaccca acgccttact acattaacct
     6661 gattcaggtg agcgtaaatg gtaaagcgtt aagtaatgcg ggagttgtgc cgcctaaaag
     6721 ccagcgtcag acaagctggt gtcaggcaat cgcaccgtgt catgtcgcct ggcgggcgat
     6781 aaatgattac ggcgggttga gtgcaaagaa ggagcaaaat ctgccctgaa acaggttcgg
     6841 aaaacgtttg cgtttttttt gccgcaggtc aattcccttt tggtccgaac tcgcacataa
     6901 tacgcccccg gtttgcacac cgggaatcca ggagagttca tgtactaccc cttcgttcgt
     6961 aaagcccttt tccagctcga tccagagcgc gctcatgagt ttacttttca gcaattacgc
     7021 cgtattacag gaacgccgtt tgaagcactg gtgcggcaga aagtgcctgc gaaacctgtt
     7081 aactgcatgg gcctgacgtt taaaaatccg cttggtctgg cagccggtct tgataaagac
     7141 ggggagtgca ttgacgcgtt aggcgcgatg ggatttggat cgatcgagat cggtaccgtc
     7201 acgccacgtc cacagccagg taatgacaag ccgcgtctct ttcgtctggt agatgccgaa
     7261 ggtttgatca accgtatggg ctttaataat cttggcgttg ataacctcgt agagaacgta
     7321 aaaaaggccc attatgacgg cgtcctgggt attaacatcg gcaaaaataa agatacgcca
     7381 gtggagcagg gcaaagatga ctatctgatt tgtatggaaa aaatctatgc ctatgcggga
     7441 tatatcgcca tcaatatttc atcgccgaat accccaggat tacgcacgct gcaatatggt
     7501 gaagcgctgg atgatctctt aaccgcgatt aaaaataagc aaaatgattt gcaagcgatg
     7561 caccataaat atgtgccgat cgcagtgaag atcgcgccgg atctttctga agaagaattg
     7621 atccaggttg ccgatagttt agttcgccat aatattgatg gcgttattgc aaccaatacc
     7681 acactcgatc gttctcttgt tcagggaatg aaaaattgcg atcaaaccgg tggcttaagt
     7741 ggtcgtccgc ttcagttaaa aagcaccgaa attattcgcc gcttgtcact ggaattaaac
     7801 ggtcgcttac cgatcatcgg tgttggcggc atcgactcgg ttatcgctgc gcgtgaaaag
     7861 attgctgcgg gtgcctcact ggtgcaaatt tattctggtt ttatttttaa aggtccgccg
     7921 ctgattaaag aaatcgttac ccatatctaa ttatttcttc gaattcctct ttataagata
     7981 atcagggctt tattttcagc cctggttgtt ttatattcat cgctgttgct tatttagaca
     8041 ttttgtactt ttattgttga ggttattaag cgaagcgaca atggattgtg ttgctgcggt
     8101 tttatagggt aggggagagg cagatgcgaa ttaaaccaga cgataactgg cgttggtatt
     8161 acgatgaaga gcacgatcga atgatgctcg atttagccaa tggtatgcta tttcgctcac
     8221 gttttgcgcg caagatgttg actccagatg ccttttctcc cgcaggcttt tgcgttgacg
     8281 acgccgcgct ctatttctct tttgaagaaa agtgccgcga ttttaattta tctaaagaac
     8341 aaaaagccga actggtgctg aatgcactgg tagcaattcg ctatctcaaa ccgcaaatgc
     8401 cgaaaagctg gcattttgtt tcccatggtg aaatgtgggt gcccatgccg ggcgatgcgg
     8461 cctgcgtttg gttgagcgat actcacgagc aggtcaattt gctggtcgtt gaatctggcg
     8521 aaaatgccgc actatgcctg ctggcacaac cctgcgttgt tattgcgggt cgcgcgatgc
     8581 agttgggcga cgccattaaa atcatgaacg acaggctgaa accgcaggtt aatgttgaca
     8641 gcttcagcct cgaacaggca gtctaacgcg ccaacttaag tgcagtcttc ggtacacagc
     8701 tacagcaaag aatggtgcca tcatcgccca ttgctgattt tttcagcggc atgacttcgc
     8761 cttctaaaag ctgaacacgg caacttccac aaatgcccgc gcggcaagaa taagggatac
     8821 gaattccctg attttctaat tgctccagca acacctgttg gttatttcca cgaaatgcct
     8881 gtccctgcca gtcaatatct acatttgcgt ccggttgttg cgtgatgttg gcggtatcat
     8941 cagcggcagc tgcgccgtaa atttttgccg gagccgttgc cagaatttcc acctcatcgc
     9001 caacgcgaat cacgccgcta ttacgggcaa ttaaattctg accaaaatcg acatcgccgt
     9061 tatcctgggc agtgcggaaa gattgcaatg tttttaatgg ttcgcctgcc ggatgttttt
     9121 gccctttttc tgggctgacg gtggtgaaaa tacagcggct acaaggttta accacatcaa
     9181 acaccacatc accaatgcga atcactttcc agcgatcttc ttcccaggct gacgcgccgg
     9241 aaaccaccag attggggcgg aattgctcca tttttacact ggccggacaa cgttgttgga
     9301 gatcacgtaa cgaggcttcg ttagcaagaa ggtaaggata gccatcagca aatgacagag
     9361 gtacagtgtt gtggcgtttc acgcgccggg tcatttgtgg ccccacccag cgtaattgca
     9421 cttcgcggga gaaaaatcca cttagccatt tgttgatcgc gtctggcgca attcgcgcgg
     9481 taaaatgtgt gccccaaact tcggttggtg cgtcttgtgt ggcgaaatca gcaaaacgaa
     9541 catatgcgct actgccatct ggtgcggtga gatgcaagcc atcatgcaca ggtgaaggag
     9601 taaaccgtac catctgggga aactggcgag cggtaataaa cgtaccgtca ggttccgtga
     9661 tcataaagat gcgatcgaag gccagaccac tgacatcagc cagagcatgt gtaagaccaa
     9721 tgccgcgcat cgatttaaca ggatgaataa aaagccgggt taatgtcgcc acggtgcggt
     9781 cctcaaatga aaataagccc tcaactttat gacatgacgc gcttattagc tataatgcgc
     9841 aacaattttc ttagcgcctg tcccattagg ctattttatt tgtcagtttg gccctgggtt
     9901 gtgctcgaaa tcctcacgta cttgtgtacg ctccggtttc tccgcgctgt ccatgtccag
     9961 actgactgca acaattacgc ctactgcggt aggctcttag agtaaaagtg acgatatgaa
    10021 ttctctgttt gccagtacgg cccgtgggct ggaagagctg ttaaaaactg aactggaaaa
    10081 cctgggggcc gttgaatgcc aggtggttca gggtggggtc catttcaagg gcgacacacg
    10141 gcttgtttac cagagcctga tgtggagccg cctggcctcg cgtattatgt tgccgctggg
    10201 cgagtgtaag gtttacagcg atttagacct ctatctcggt gttcaggcga tcaactggac
    10261 agagatgttt aatcctggcg cgaccttcgc tgtccacttc agtggtttga atgacaccat
    10321 ccgcaacagt cagtacggcg cgatgaaagt gaaagacgcg atcgtcgatg ctttcacgcg
    10381 gaaaaatctg ccgcgtccaa atgttgatcg cgatgcgccg gatatccgcg ttaacgtctg
    10441 gctgcataaa gaaaccgcca gtatcgctct tgatctgagt ggtgatggtt tacatctgcg
    10501 tggctatcgc gatcgtgctg gtattgcgcc gatcaaagaa accctggcag ccgctattgt
    10561 gatgcgaacc ggctggcagc caggaacacc gctgctcgat ccgatgtgtg gttccggtac
    10621 gttgctgatt gaagcggcga tgctagcgac cgatcgcgca ccaggcttgc accgtgggcg
    10681 ttggggcttt agcggctggg tgcagcatga tgaagctatc tggcaggaag tgaaagcgga
    10741 agcgcaaact cgtgcccgta aaggcctggc tgagtatagc tctcattttt acggttcgga
    10801 cagcgacgca cgggtgattc aacgtgcgcg cactaacgcc cgtcttgcgg ggattggtga
    10861 actgatcacc tttgaggtga aagatgtcgc gcaactgacc aatccgctgc cgaaagggcc
    10921 gtacggcaca gtgttgagca acccgccata cggggaacgt ctggacagcg aaccggcgct
    10981 gattgcgctg catagcctgc tgggtcggat catgaaaaac cagtttggtg gctggaatct
    11041 ctctttgttt agtgcctcgc cggatctgct aagctgcttg cagctgcgtg cagacaaaca
    11101 gtacaaggcg aaaaacggcc cgctggactg cgtacagaaa aattaccacg ttgccgaaag
    11161 cactccagac agcaaaccgg cgatggtagc ggaagactac gccaaccgcc tgcgtaagaa
    11221 cctcaaaaaa ttcgagaagt gggctcgcca ggaaggaatt gaatgttacc gcctgtatga
    11281 cgccgatcta ccggaatata acgttgccgt tgaccgttat gccgactggg tggtggtgca
    11341 ggagtatgcg ccgccaaaaa ctattgatgc tcacaaagcg cgtcagcgtc tgttcgatat
    11401 tatcgctgca accatttcgg tactggggat tgcgccaaac aaactggtgc tgaaaacccg
    11461 tgaacgccag aagggcaaaa accagtacca gaaactgggc gagaagggcg agtttcttga
    11521 agttaccgaa tataacgctc acttgtgggt gaacctgacg gattatctcg atactggtct
    11581 gttcctcgat caccgtatcg cccgccgtat gctcggtcag atgagcaaag gcaaagattt
    11641 cctcaacctg ttctcttata ccggtagcgc caccgtgcat gcgggattag gcggcgcgcg
    11701 cagcaccacc accgtggata tgtcgcgtac ttatctggag tgggcagaac gcaacctgcg
    11761 tctgaatggc ctgaccgggc gtgcgcatcg cctgattcag gccgattgcc tggcgtggct
    11821 gcgtgaggca aatgaacagt ttgatctgat ctttatcgat ccgccaacct tctctaactc
    11881 aaaacgaatg gaagatgcgt ttgatgttca gcgcgatcat atggcgctga tgaaagattt
    11941 gaaacgtctg ctgcgtgcag gtgggacgat catgttctcg aacaacaaac gtggcttccg
    12001 tatggatctc gacggcctgg caaaactggg actgaaagca caagaaatta cgcaaaaaac
    12061 gctctcccag gatttcgccc gtaaccgcca gatccacaac tgctggctga ttaccgcagc
    12121 ctgaaaggaa tagtaatgtc attaatcagt atgcatggcg catggctgtc gttcagcgac
    12181 gcgccgcttc tcgataacgc agaactgcat atcgaagata acgaacgtgt ttgtctggtg
    12241 ggtcgcaacg gcgcgggcaa atcgacgtta atgaaaatcc tcaaccgtga acaagggctg
    12301 gatgacggtc gcattattta cgagcaagat ttgattgtag ctcgtctgca acaggagccg
    12361 ccgcgtaacg ttgagggtag cgtttatgat ttcgttgccg aaggcattga agaacaagcg
    12421 gaatatctga aacgctatca cgatatttcg cgcctggtga tgaacgaccc gagcgagaaa
    12481 aatctcaacg aactggcgaa ggttcaggaa cagttggatc accacaacct gtggcagctg
    12541 gaaaaccgca tcaacgaagt gctggcgcaa ctggggttag atcctaatgt tgcgctgtcg
    12601 tcgctttccg gcggctggtt gcgtaaagcg gcattaggac gcgcgctggt gagtaatccg
    12661 cgcgtgctgt tgcttgatga accgacaaac cacctggata ttgaaaccat cgactggctg
    12721 gaagggttcc ttaaaacctt caatgggacg attattttca tctcccacga ccgttcgttt
    12781 atccgcaata tggcgacgcg cattgttgat ctcgatcgcg gcaagctggt gacctatcca
    12841 gggaattacg accagtacct gcttgaaaaa gaagaagccc tgcgcgtgga agaactacaa
    12901 aatgccgagt tcgatcgcaa actggcgcag gaagaggtgt ggatccgcca ggggatcaaa
    12961 gcacgccgta cccgtaatga aggccgcgta cgcgccctga aagcaatgcg tcgcgagcgt
    13021 ggtgaacgtc gcgaagtgat gggtaccgca aagatgcagg tggaagaggc cagccgctcc
    13081 ggtaagatcg ttttcgaaat ggaagacgtt tgctaccagg ttgacggtaa gcaactggtg
    13141 aaagattttt cggcccaggt tctacgtggc gacaaaattg ccctgattgg tccgaatggg
    13201 tgcggcaaaa ccacgctgct gaaactgatg ctcgatcagc ttcaagcgga cagcgggcgt
    13261 attcacgttg gcaccaaact ggaagtggct tatttcgatc agcaccgcgc ggaactggat
    13321 cccgataaaa cggtgatgga taaccttgcc gaaggtaagc aagaggtgat ggttaacggc
    13381 aagccacgcc acgtattggg ctatttgcag gactttctgt tccatccgaa acgggcgatg
    13441 acgccggtac gtgcgctttc tggcggtgag cggaaccgct tgctgctggc gcgtttgttc
    13501 ctcaaaccaa gcaacttatt gattcttgac gaaccgacca acgatcttga tgtcgaaacg
    13561 ctggaactgc tggaagaact gatcgacagc tatcagggca cggtattgct ggttagccac
    13621 gatcgtcagt ttgtcgataa caccgttaca gaatgttgga tcttcgaagg cggcggtaaa
    13681 attggtcgtt atgtcggcgg ttatcatgat gcccgtggtc agcaagagca gtatgtggcg
    13741 ctcaaacagc ctgcggtgaa aaaaaccgaa gaagccgccg cggcaaaagc agaaactgta
    13801 aaacgcagca gtagcaaact aagctataaa ttgcagcgcg aactggagca gctaccgcaa
    13861 ttgctcgaag atctggaggc gaagctggaa gccctacaga cgcagatggc ggatgcttcc
    13921 ttcttcagtc agccacatga gcagacacaa aaagtgctcg ccgatatggc tgctgcagag
    13981 caggagctgg agcaagcctt tgaacgctgg gagtatcttg aagcgttaaa aaatggtggc
    14041 tgatcgcaaa atagtcgatt taaacagaga cagctgacaa cggtaaaatt gcctgatgaa
    14101 ctgggtttat caggcatgaa aaccaacact ttgttagcca tctgtaaaat agcgcatcat
    14161 taaggagtac caatgtgcga acatcatcat gccgcgaagc acatcctgtg ctcgcagtgt
    14221 gacatgctgg tggcgttacc gcgccttgag catggtcaga aagcggcatg tccccggtgt
    14281 ggcacaacgt taaccgtggc gtgggatgcc cctcggcagc gtccgaccgc ctatgcgttg
    14341 gctgcactgt tcatgctgtt gctgtccaac ttgtttcctt ttgtgaatat gaacgttgca
    14401 ggagttacca gtgaaattac attactggaa attcccggcg tgcttttttc tgaggactac
    14461 gccagcctcg gcaccttttt cctgttgttt gtgcaactgg ttcccgcgtt ttgtctgata
    14521 accattctgt tactggtgaa tcgcgcggaa ttaccggtcc gtttaaaaga gcaactggca
    14581 cgggtgcttt ttcaactcaa aacctgggga atggcggaga ttttcctcgc tggtgtgctg
    14641 gtcagtttcg ttaaactgat ggcttacggc agcattgggg taggcagcag ctttctcccc
    14701 tggtgtttat tttgtgtcct gcaactgcgc gcttttcagt gcgttgatcg tcgctggtta
    14761 tgggacgaca tcgccccgat gccagaactg cgccagccgc taaaaccagg cgtcacgggg
    14821 atacgtcagg ggctgcgttc gtgctcctgt tgtacggcaa tccttcctgc tgatgaaccc
    14881 gtgtgcccgc gttgtagtac caaagggtac gttcggcgta gaaacagcca gcagtggaca
    14941 ctcgcgctgc ttgtaacgtc catcatgctg tatcttccgg ctaatatttt gcccatcatg
    15001 gtgacggatt tattaggctc gaagatgccg tcgacgattc tcgctggggt cattctgtta
    15061 tggagcgaag gctcttatcc cgtcgctgcg gtgatctttc tggccagtat tatggtgcca
    15121 acgttaaaga tgatcgccat cgcgtggctg tgttgggatg ccaaagggca tggcaagcgc
    15181 gacagtgaaa gaatgcattt gatttatgaa gttgttgagt ttgtaggccg ctggtcgatg
    15241 attgacgttt tcgttatcgc ggtgctctcg gcgctggtgc gtatgggagg tttaatgagt
    15301 atttatccgg caatgggtgc attaatgttt gctttagtcg tcataatgac aatgttttct
    15361 gctatgacgt ttgacccgcg tttgtcgtgg gatcgtcaac ctgaatcaga gcatgaggag
    15421 tcctgacagt atggaatcta ataatgggga agccaaaatc cagaaagtga agaactggtc
    15481 tcccgtgtgg atatttccta tcgtcacggc gctcattggg gcctgggttc ttttttatca
    15541 ttacagccat cagggactgg aagtgaccct gatcaccgcg aatgcggaag gaattgaagg
    15601 tggcaaaacc accattaaaa gccgtagcgt tgacgtcggc gtggttgaaa gcgccacact
    15661 ggctgatgat ttgacgcacg ttgaaatcaa agtgcggctg aattctggta tggaaaaatt
    15721 gctgcataaa gacaccgtct tttgggtggt gaaaccgcag attggtcgcg aagggattag
    15781 cggcctggga acgctgctgt ctggagttta tatcgaactg cagccaggcg cgaaaggcag
    15841 caaaatggat aaatacgatt tgctggactc gccaccgttg gccccgcctg atgcgaaagg
    15901 tatccgtgtg attctcgata gcaaaaaagc cgggcagctc tcgccaggag atccggtgct
    15961 gttccgtggc tatcgggtag gttcggttga aaccagcacc ttcgatacgc aaaaacgcaa
    16021 tatcagttat caactgttca tcaatgcacc ttatgaccga ctggtgacca gcaatgttcg
    16081 cttctggaaa gatagtggca ttgcggttga tctgacgtca gcggggatgc gtgtggagat
    16141 gggctcattg acaacgctgc ttagtggcgg tgttagcttt gatgtgccgg aaggtctgga
    16201 tttagggcag ccagtggcac cgaaaacagc tttcgttttg tatgatgatc agaagagcat
    16261 tcaggattcg ttgtacaccg atcacattga ttatctgatg ttctttaaag attcggtacg
    16321 cggtctgcaa ccgggagctc cggtagaatt ccggggtatt cgcctgggta ccgtaagcaa
    16381 agtgccattc tttgcgccga atatgcgtca gacatttaac gatgattacc gtattccggt
    16441 actgattcgt atcgagccag agcggctgaa aatgcagctt ggcgaaaatg cggatgttgt
    16501 tgagcacctt ggcgaattgt tgaaacgtgg tttacgcgga tcgctgaaaa ccggaaacct
    16561 ggtcactggt gcactgtatg ttgatcttga tttctatcca aatacgcctg caataaccgg
    16621 tattcgtgaa tttaatggtt atcagattat cccgactgtt agcggcggcc tggcgcaaat
    16681 ccagcaacga ctgatggaag cgttggataa gatcaacaaa ctgccattga atccgatgat
    16741 tgaacaggca accagtacgc tttctgaaag tcagcgcaca atgaaaaacc tgcaaacgac
    16801 gctggatagc atgaacaaga tcctcgccag ccagtcgatg cagcagttgc cgacggatat
    16861 gcagtcaacg ttgcgtgaat tgaatcgcag catgcagggc ttccagcctg gctccgcagc
    16921 ctacaacaag atggtggcgg atatgcagcg ccttgatcag gtgttgcgag aactgcaacc
    16981 ggtgctgaaa acgctcaatg agaagagtaa cgcgctggta tttgaagcga aggacaaaaa
    17041 agatccagag ccgaagaggg cgaaacaatg aaaaagtggc tagtgacgat tgcagcactg
    17101 tggctggccg gatgcagctc cggcgaaatt aataaaaact attaccagtt acctgtggtg
    17161 cagagcggta cacaaagtac cgccagccag ggcaatcgtc tgttatgggt agagcaggtc
    17221 actgttcctg actatctggc ggggaatggt gtggtttatc aaaccagtga tgtgaagtat
    17281 gtgattgcca acaacaactt gtgggccagc ccgttggatc aacaattgcg caacaccctg
    17341 gttgccaacc tgagtacgca actgcccggc tgggtggttg cctcccagcc tctgggaagc
    17401 gcccaggaca cgctcaatgt taccgtaacg gagtttaacg gtcgctatga tggcaaggtc
    17461 attgtcagtg gtgagtggct gttgaaccac cagggacaac tgatcaaacg tccgttccgt
    17521 ctggaaggag tgcaaactca ggatggttac gatgagatgg ttaaagtact ggccagtgtc
    17581 tggagtcagg aagccgcttc tattgcgcaa gagataaagc gtctacctta attataaaga
    17641 tttgtaaata taaccgtctc cggtatgttg cctgaggcgg tttttttgtt tctaacgtgc
    17701 ggaaaaagtt gttcctcttt acattttttg tacaaccgac atacccgtgt agctcacaaa
    17761 tatgacagtg gcgtgaattt tgcgcattga cggcagttat gattcgcggt attgcttaac
    17821 tgtgattgca catttagtaa tcactgtttt cttttccacc agaaaccagt atgagggaaa
    17881 cgaggcatga agagacaaaa acgagatcgc ctggaacggg cacatcaacg tggttatcag
    17941 gccggcatcg ccggacgctc aaaagaaatg tgtccctatc agacgctgaa tcaaaggtca
    18001 caatggctgg gaggctggcg agaagccatg gcggacaggg tagtaatggc ctgattctgt
    18061 ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct tttgatactc tgtctgatta
    18121 taatcagaag gcagaagtat cctggaacag accgactttc aggtcgctgg cggtatagat
    18181 caaacgacca tcaaccagca cttctccatc cgccaggccc ataatcagac gacggttaac
    18241 aatgcgttta aagtgaatac ggtaggtcac ttttttcgct gtcggcagta cctgaccagt
    18301 gaatttcact tcgccaacgc ccagtgcgcg gcctttacct tcgccgccca gccagccgag
    18361 gtagaaccct accagctgcc acattgcgtc caggcccagg catcccggca taaccggatc
    18421 gccaataaag tggcatccga agaaccacag atccggattg atatccagtt ctgcttcaac
    18481 ataccctttg tcgaagttac cacccgtttc ggtcattttg accacacggt ccatcatcag
    18541 catattcggt gctggcaatt gcgggccttt agcgccaaac agttcaccgc gaccagaggc
    18601 aagaaggtct tcttttgtat aggattcgcg tttatctacc atgttctctg taagccttat
    18661 tttattgaag cacgcaggat agctaacacg tgtacgctga acaagtccga tcagttcgga
    18721 ataaaccagt tcagccaacg taatggccat ggaaaacggt gacgtccttc ctgttgcgat
    18781 gcttgcgcga tacgttcctg gatggtttgc atcagcgtcg tttggccttc gccatcccac
    18841 accagattta ataataacgg cagtgcgtca gtcacatcgt ctactgccca gatggtgaat
    18901 ttgccttctt ctaccgcttt cactagttca ctgtgaagac ttaaatggcg aacgttagct
    18961 gtggggataa tgacaccttg tttcccggtt aactcacgtt gctggcaaat agcaaagaag
    19021 ccttcgattt tctcatttaa accaccgacc ggctgggcgc gaccgaactg atcgactgaa
    19081 cctgtgatag cgatactctg attcaccggc acatcggcga gggcgcttat cagggcgcag
    19141 agttcagcca tcgaggcact atctccatca acttcactgt atgactgctc aaatgtcagc
    19201 gatgctgaga aggggatctg ttgctcaagc tgtagttccg acatcaggaa cgcttgcatg
    19261 atcatcatcc ctttcgcatg gatattgccg ccaagctccg ctttgcgttc gatgtcggtg
    19321 aattcaccat cgccaatatg cacaacgcag ctaatgcgag aaggttcgcc aaaagcgcgt
    19381 ggatgacccg gaaattcaat gaccgaaagg gcgttaattt gcccgatgcg ttcgccttcg
    19441 gtttcaatca ggatttgctc ctgaaggatc tcatcctgca tacgttcagc gaggaaacct
    19501 tcgcgccatt cacgctgctg cagcattaag tttagctgct cgccggagaa ggtgtcgcca
    19561 tcacacaggg aggcgacctc tttacactgg cggaggatcc actgcgggct aagcggaagt
    19621 gtttcttgtt caccggtgta gcgtgctgct tcgcggataa gtaccggcca ggcatccgct
    19681 cccggtgcag gtaagtgatt atgtctggcg gtaaatgtca cccagcgaca ccactgggtt
    19741 actgactccg catcgacaat ctgcagagta tcttcaaatt cgctataaat agcctgctct
    19801 gaaagctctg gctccatctc ctggaaatca gccaatgatt cgcgttcgcc taccagaatg
    19861 actttcagct tcaatggcat cgaaggcaca gagacgggga gagggcgcga ctcatcaaac
    19921 gcaacccagt caaaacgctc gcggttaacg atatttttca gccgcatcca cagcagaggt
    19981 tgcgccagca gtgtacgcaa agagatgatg agaataccgc catttgcctg atgcaccaga
    20041 ccaggctgca gggtaatgtc gccattaaac tggcgcaggc agccaaagag ttgctccgct
    20101 tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa aattatcatc tgcactcact
    20161 gcgtgacgta agcggatgga gtggccggaa acctcatagt gaccgcccac cagttggcct
    20221 gcatcgcttt gtagcgtacg cgcggcattg gcaataagat tcagatactc agactcttcc
    20281 ggggccttcg ccagcataaa agaggaggat gctcgcgtat gcagcaactg ctccagcgca
    20341 aattgcagcc gcggttgagt atcactgaat aaaggatcgt tttcgtcaat caaatgtggc
    20401 tgagcaaata tttcctgata gctatcggta tcaggaacca ggtcacgcca tgcaagtttc
    20461 gtaatggtca aagttgatgt tttttagtct gttgtcaaag ccgcgattat accgtaaccg
    20521 gcactacagc acacgtagaa agcaccgaca atactccagg catgggcgtt aaagctcaca
    20581 ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag agttacacgg
    20641 taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg gttggaaatg
    20701 gaagtatctg gtgaagaagc atcgcgaagg ggagttaatc acccgttaca tagaagccag
    20761 tgccgcccag gaagccgtcg atgtgttgct ctcgctggaa aatgaacccg tgctggtaaa
    20821 tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc aaaccattcg
    20881 ggcaagacga aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa aatcgatcga
    20941 tctggaattt attgtctggc aacgtctggc tggtcttgcg cagcgtcgcg gtaaaacgct
    21001 ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat acgcgaataa
    21061 aatgtcttct ctgaagcagg atctgcaggc attgctgggt aaggaataac tgacgaaagt
    21121 cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc taccagacga
    21181 gaacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg atctctacgc
    21241 gacgatccgg agccaggcag tcgatcagtg cagcacgctg tttcacgttg tcacaggtgt
    21301 tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct gccgggatac
    21361 ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga ccctggttgt
    21421 aagcgtcaga accgatgcgg tcggtgtaac ccagaacaac tacggaaccg tctttcggat
    21481 ccaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc ggtttcaggg
    21541 ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg gtctgtactt
    21601 ccggtgccgg agctggagcc ggagcaacta ctggagctgc ttcgccctga ccgaaacggt
    21661 aggaaacccc caggctcagc atgccgttgt ccggacgagt gccgatggtg tgtgcgtcac
    21721 cgatgttgtt ggtccactgg tattccagac gggtagcgat ttcaggagtg atcgcgtact
    21781 caacaccgcc agcgaagacc ggagaaacgc cggtgtcgtg gtttttacca taaacgttgg
    21841 atttagtgtc tgcacgccat accataccac ccagacgagt gtagatgtcc aggtcgtcag
    21901 tgattgggta acccagttta gcggtcagtt gaacgccctg agctttgtat gcaccgtttt
    21961 caacgctgcc tttgtacggc atacgaccta accagtcgta acccatttca aagccaacat
    22021 acgggttaac ctggtaacca ccaaaagcac cagcgcccag ttggttttca tgggtcgggc
    22081 cattgttgtt gatgaaacca gtgtcatggt actgggacca gcccagttta gcaccagtgt
    22141 accaggtgtt atctttcgga gcggcctgcg ctacggtagc gaaaccagcc agtgccactg
    22201 caatcgcgat agctgtcttt ttcatttttt gcgcctcgtt atcatccaaa atacgccatg
    22261 aatatctcca acgagataac acggttaaat ccttcaccgg gggatctgct caattaactc
    22321 taccgatatc ttcggcttat gccgagcacc cctggcgatg taaagtctac aacgtagttg
    22381 aaaacttaca agtgtgaact ccgtcaggca tatgaaaaaa aagtcttgta taaggtatgt
    22441 ttaatctttt ttgtcagcga caatttacag aagaaaatcg cggaaaccgc ttcagacaag
    22501 cctccgcaag gaaaattagt cacgactgaa agcattggct gggcgacaaa aaaagttcca
    22561 ggattaatcc taaatttact taatgataca aattagagtg aatttttagc ccggaaagtt
    22621 gtctcgtggc gtgagaggat gcgcttaccg gacgcataat aaaccccata gcgttacctt
    22681 catttgccgc atcaacaagt tcagcatgct cttcttcagt caaatcatct gccaaccaac
    22741 cgatcaccac actgtaattg cccgtgcgta aagcgcgaac cattgactcc acagtgtggc
    22801 aaggggagag ctggctaatc tgcattactt tcgttaaggg tagcccagat gcctgaaccc
    22861 attcccgact cagtttttgt tgcggtgtta accagagttg ccagcgcgat tgctgaccga
    22921 gttgctgtaa caatggcaac agtagaagtt gcgtcatcat gggctgatct tcgcgataga
    22981 caacttcact gataagcccg gctgtagtgt tttccgtaga gacacgcgca attttacttg
    23041 ctgcggatga gaacgacgaa gaacgatgtg catagcctga agtgtacata atcaatccag
    23101 cccctatgag ttactgtatg gatgtacagt acatccagtg acaacaaaga tcaaccctat
    23161 tttcggaaag agcctcgcaa attttgtcgt tggtgacggg aaaacataaa ttaatcttgc
    23221 cccttaagaa taagttgcct attttcgtag ttaacggatc cgttaatgtg aatcattctt
    23281 ttatgttatg attttaaaag gaattttatg aaaagcctct cctataagcg gatctataaa
    23341 tcacaagaat acctggcaac gttgggcaca attgaatacc gatcattgtt tggcagttac
    23401 agcctgaccg ttgacgacac ggtgtttgcg atggtttctg atggtgagtt gtatcttcgg
    23461 gcttgtgagc aaagtgcaca gtactgtgta aaacatccgc ctgtctggct gacatataaa
    23521 aagtgtggcc gatccgttac cctcaattac tatcgggttg atgaaagtct atggcgaaat
    23581 caactgaagc tggtgcgtct gtcgaaatat tctctcgatg cagcgctgaa agagaaaagc
    23641 acgcgcaata cccgggaaag actgaaagat ttgcccaata tgtcttttca tctggaagcg
    23701 attctcgggg aggtggggat taaggatgta cgggcgttac gtatacttgg ggcaaaaatg
    23761 tgttggttgc gactgcggca gcaaaacagt ctggtgacag aaaagattct gtttatgctt
    23821 gaaggtgcca ttatcggcat tcatgaagct gcgctcccgg tggcacgccg ccaggagctt
    23881 gcagaatggg ctgactctct tacgccgaaa caggagtttc ctgcggaact tgagtaatct
    23941 ggcgttgcag acgaccaatc tcaggcagta atgcaatcaa taatccaact tgttgtacga
    24001 ccagaggttc tttgctgtct gcccgtggtt caagttgctg catccgctgt ttcaggctag
    24061 ctaatgcctc attgacgcgt tcttcatcag caggttgatg atgtaacgcg tcatcaacat
    24121 agcaaactgc gtcatcaaga aacgccagaa tttcaggatt agttaactgc tcccggtgag
    24181 caccgagggc tgagatatag ctggtaaacg tatggttaag gcacagcaac cgaaacgcgg
    24241 cttcgcgaat ttgcggggta acgttcggct cgctggacat atttgatacc accgacgcca
    24301 gctcagcatc acggttgtgt gcatcgcggc gggcaatacg atacgccaga cggttatcac
    24361 gcccctgatg gtattgctcc agtatggcat cgagataccg acagttggcc tctgtggcgc
    24421 gttcgagcat gcgcggcaga ttgcgaaact gccagtcagg ccagatgtag ctcactgccg
    24481 cccacgcaat ggcacaacca atcagcgtat cgattacgcg aggtaacgct acttcaaaac
    24541 cttcacccag taagttaaaa cacagtagca ccaaaagtgt gatgaacatc gttgcatgag
    24601 cgtattgcac gttacggaag gcaaaaaaga gcacgccggt aataaccagc agcaccagct
    24661 gcccttccag tgatggcaca aaccacagca caggaatgcc aatggcgata cctaccagcg
    24721 taccaataat ccttaacttc aggcggtggc gcgtggcgtt atagtttggc tggcagacaa
    24781 acaaacttgt cagcaagatc caatacccgt gatgcattcc ggttatctga atgatggcgt
    24841 agccgaagca caataccagc gacattctta ccgcatgacg gaagagggcg gattccggcg
    24901 tgaagtgacg gctaagacgc agccagatat cactcaaccc gtgcgggctg tcatcagcga
    24961 gctcattttc gtcattatta tggggtagtg cctgggcctg ttctgattca attgttgcca
    25021 gttgggcatc aatggcgcgt aaattgttca gcaaaaatcc cagtgttttg agtaaatcgg
    25081 cgggtgcgcc gttatcgcgc atccgctcca gcgcagcatc aatatgcgta aaagcgcgct
    25141 caaaatgcgg atcatgttga taaggctgac gcaacaaaat acagcgtgac agttgctggc
    25201 acgcctggcc ctgcatcgac atcagccgct gaaaacggaa cagcacgtcg ctgtggcgaa
    25261 aatgttcacg caatgtttga tactgaatat gagaagagct ggcacgctcg tgaatatcct
    25321 gtgcgacaaa gtaataatgc agcgtgcgac gcgttccccg ttgaccacga tcgccacgta
    25381 agcgggtcag cagcgagagt ttcgtctgat tcaatgtcgc catcagcaga ccgttggcga
    25441 gagccaaatc gtacagcggt gcctggcttt catcttcaat atcaggatca aacatgcgcg
    25501 acttgagctc aagataacgc gccagttgtt catagcaacg cgccaggttg tcctgcagcg
    25561 ggcggaccgg gaacagcaga tgaccaataa gtgtcaggac gttgtaccag acggcaccgg
    25621 ccagcagata catcggctgc tgataccagt gctcatacag tgatgttccc aacatagtgt
    25681 aaatggcgat cagcaatgca ccgaaggcaa ttgttgcata gcgttgaccc agaccgccga
    25741 gcaaaatgaa gccgctggta gagagcgtta agccaatcgc aaatagccag ggccagggaa
    25801 acagcaattc tactgaggcc gaggcgataa aaaagcagaa cagcgtaatg atgaggttac
    25861 gcaaacgtcc cgccagtcgg tcatcgagat cggtcagcgc cgctgccacc atccccagcg
    25921 ttagcggaat cgtcagtttt acatcaccca gccaccacgg aaacgctgtg gttccacaaa
    25981 gcgcaataaa aatacgcgcg taatacagcc aggcgctgtt ccaggtatag cgtttgagca
    26041 aaggacttag cataaaggcc atattagtgg attattcaaa acgacgacgt gcattggctt
    26101 cacgcgcagc ttgcgctgtt tctaccgata ccacgcgacg accaaccggc catagtgcaa
    26161 tagcggcaat tttaaagttc gcaatgccga caggaatgcc aatgattgaa atacattgtg
    26221 cgatgcccgt tgcaatgtgc atcaggcata accaccagcc aaagaaaatc agccagaaaa
    26281 tattcaatac cgtaccgcca gtattcagca gcacattttt gccagccggg ttcagttcat
    26341 cgacatgaat agcttcattg ccataaggca ccagagatag tttagtgatc tcccagcagg
    26401 atcgtgtcag cggtaaggta aaaatcagca caatactgac cagagtcgcc aacagccagc
    26461 ccagagtggt ggcaaatccg ccaagcacaa agttcagaat gttcaaaacg gtacgcataa
    26521 aacctcgctt tactgtggtt ttcagtaatg ggcggcaatt gtaacgtttt tttgggctgg
    26581 agcacgtttt ctctgacggt tacactgata agaaataatt tcgtgtggat ctacagagtc
    26641 atggaactga aagcgacaac gcttggaaaa cgtctggcac agcaccctta cgatcgggcg
    26701 gtgatcctca atgccgggat taaagtctcc ggcgatcgcc acgaatacct tattcctttc
    26761 aatcaattac tggcgattca ctgtaagcgc ggtctggtat ggggcgagct ggaatttgta
    26821 ctgccggacg aaaaagtggt gcgtctgcac ggcaccgaat ggggcgagac gcagcgtttt
    26881 taccatcatc ttgatgctca ctggcggcgg tggagtggcg agatgagcga aattgcgtct
    26941 ggtgttttac gccagcaact ggatttgatt gccacgcgca ctggagaaaa taaatggctg
    27001 acgcgtgagc aaacctctgg cgttcagcaa caaatccgcc aggctttgtc ggcgttgccg
    27061 ttgccggtta accgactgga agaattcgat aactgccgtg aggcgtggcg taaatgtcag
    27121 gcctggttga aagatattga aagcgctcgg ttgcagcata accaggcgta taccgaagcc
    27181 atgcttaccg agtatgcgga ttttttccgc caggtcgagt cttcaccgct gaatccggcg
    27241 caggcccggg cagtcgttaa tggcgagcat tctctgttag tgctggcagg tgcaggaagc
    27301 ggaaaaacgt cggtgctggt ggcccgtgca ggctggttgc tggcgcgtgg tgaagcgtcc
    27361 cctgagcaaa ttttattgct ggcgtttggt cgcaaagccg ctgaagagat ggacgagcgg
    27421 attcgcgaac ggctacatac cgaagacatt accgcacgca cgtttcatgc gctggcgctg
    27481 catattattc agcagggcag caaaaaagtt ccgatagtca gcaaactgga aaatgatacc
    27541 gctgcccgtc atgaactctt tattgctgag tggcgcaagc aatgcagcga aaagaaagcg
    27601 caggcgaagg gctggcggca atggctgacg gaagaaatgc agtggtcagt gccagaaggt
    27661 aacttctggg atgatgaaaa attacagcgt cgccttgcct ctcgcctcga tcgctgggta
    27721 agtctgatgc ggatgcacgg tggtgcacag gcagaaatga ttgccagtgc acccgaagag
    27781 attcgcgatc tgttcagtaa acgtatcaag ttgatggccc cgttattaaa agcctggaaa
    27841 ggtgcgctga aggcagaaaa cgctgtcgat ttttcgggcc ttattcatca ggcgattgtg
    27901 attctggaga aaggtcgctt tatcagcccg tggaagcata ttctggttga tgaatttcag
    27961 gatatctcgt cgcagcgggc agcgttgtta gcggcattac gcaagcaaaa cagtcagacg
    28021 acgttgttcg ctgttggtga tgactggcag gcgatttacc gattcagcgg tgcgcaaatg
    28081 tcgctcacca ccgctttcca tgaaaacttt ggtgaaggcg aacgttgtga tttagacacg
    28141 acttaccgtt ttaacagtcg tatcggtaag gtggcaaacc ggtttattca gcagaaccca
    28201 ggccagctga aaaagccgct aaacagctta accaatggag acaaaaaagc cgtcacgtta
    28261 ttggatgaga gtcaacttga cgctttgctg gataagctct ctggttatgc caaaccggaa
    28321 gagcgcattc tgatcctggc gcgttaccat cacatgaggc ctgccagcct ggaaaaagcg
    28381 gcaacacgct ggccgaagtt gcaaatcgac tttatgacca ttcatgccag caaagggcaa
    28441 caggcggatt acgtcatcat cgttggcttg caggagggaa gtgatggttt tccggctgcg
    28501 gcgcgggagt cgattatgga agaggcgcta ctgccaccgg ttgaggattt cccggacgct
    28561 gaagaacggc ggttaatgta cgtggcgctg acccgggcac gccatcgggt atgggcactg
    28621 tttaacaaag agaatccctc tccctttgtg gaaatactga aaaatctgga tgtgccggtg
    28681 gcgagaaaac cgtaagaaac aggtggcgtt tgccacctgt gcaatattac ttcagacggt
    28741 ccgcgagata acgctgataa tcggggatta gaatatcgac cgcgtcgttg aaatgcggcg
    28801 actggattat gaagtctgcc gttgccacgt tggtggcgac cggaatgttc cataccgtcg
    28861 ccagacgcag caaggctttc acgtcaggat cgtgcggcac ggcatttagt ggatcccaga
    28921 agaaaatcaa tacatcaatt ttcccttctg agatcaatgc gccaacctgc tggtcacccc
    28981 ccattgggcc actcaacatc gcgttgacgt tcatgccggt cgcgcgggaa attaagttac
    29041 cggtagtgcc tgttgcatac agtacgtgtt gttccagtaa cggttgatgc cgttccaccc
    29101 agctcatcag catttgtttg cagtgatcgt gtgccaccag cgcaatatgt ttccgcgcag
    29161 gtaaagtgcg agtcgtcagt tccataatgt acatccgtag ttaactttcc tacagattac
    29221 tgtaagcact tatcgctgca agataaagac cgaaaaagcc tgcgcacagg cacaaaaatc
    29281 tcaggaagat ggttgttttt ccgcccactg caggaaagta tttcgcgttt gtgggtcagc
    29341 cagtttaaac caatacttca gccgttgttc tgtgagcacc tgagactgcg gtggaatagc
    29401 atccagctcg gaaacacccg aaagcagcgt actgtcatct gccatcatcg tggcaaattg
    29461 cggtagtgaa gcgcgtttcg cggatttgtt atagcgttca acctcgacct cataatcaat
    29521 cgtttttgcc gttgaggtaa tggcgagaat atccagcttt accggaatcg gcgtcgcatc
    29581 gccatccaac aattcaaggc gcggcgcggc atcaaaatgg ttagcttccc gctcattctc
    29641 caggcgaggc aggcgaaaat tgacctggtt gatgagctgg gtattaaaac tgaccaccag
    29701 cggtggggag atatacagcc gttcttcact attggaaaga tgaatcgtct tctcaacgcg
    29761 aaacactaac tgatgcggtc cattatccag ttcaatgctg tccgcaccgc gcagcagaga
    29821 gctggaaacc ttcttgccgt cgagtaccag aagatcgaca tcggttgaaa gccgcagcgt
    29881 ggtggcaaaa acggataccg gcaaacataa tgcaattaag gtggtcacga tgccggtttt
    29941 cataggtagc tcctgtcaaa agaccgcccg aataataatg tgtcaaaatt tttctttaat
    30001 c
//
LOCUS       DAJVQG010000034        29538 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJVQG010000034 DAJVQG010000000
VERSION     DAJVQG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29538
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.034"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..689
                     /gene="dgt"
                     /locus_tag="OQM83_001396"
     CDS             <1..689
                     /gene="dgt"
                     /locus_tag="OQM83_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HCS1519691.1"
                     /translation="EDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRS
                     NSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLL
                     KLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRF
                     PIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWD
                     EYRRLMAVEQ"
     gene            819..2243
                     /gene="degP"
                     /locus_tag="OQM83_001397"
     CDS             819..2243
                     /gene="degP"
                     /locus_tag="OQM83_001397"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCS1519692.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2398..3555
                     /gene="cdaR"
                     /locus_tag="OQM83_001398"
     CDS             2398..3555
                     /gene="cdaR"
                     /locus_tag="OQM83_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HCS1519693.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3644..4030)
                     /locus_tag="OQM83_001399"
     CDS             complement(3644..4030)
                     /locus_tag="OQM83_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCS1519694.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="OQM83_001400"
     CDS             complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="OQM83_001400"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HCS1519695.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="OQM83_001401"
     CDS             complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="OQM83_001401"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCS1519696.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="OQM83_001402"
     CDS             complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="OQM83_001402"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCS1519697.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8648..9442)
                     /gene="map"
                     /locus_tag="OQM83_001403"
     CDS             complement(8648..9442)
                     /gene="map"
                     /locus_tag="OQM83_001403"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCS1519698.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFIEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9810..10535
                     /gene="rpsB"
                     /locus_tag="OQM83_001404"
     CDS             9810..10535
                     /gene="rpsB"
                     /locus_tag="OQM83_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCS1519699.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10793..11644
                     /gene="tsf"
                     /locus_tag="OQM83_001405"
     CDS             10793..11644
                     /gene="tsf"
                     /locus_tag="OQM83_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCS1519700.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11791..12516
                     /gene="pyrH"
                     /locus_tag="OQM83_001406"
     CDS             11791..12516
                     /gene="pyrH"
                     /locus_tag="OQM83_001406"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCS1519701.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12808..13365
                     /gene="frr"
                     /locus_tag="OQM83_001407"
     CDS             12808..13365
                     /gene="frr"
                     /locus_tag="OQM83_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCS1519702.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13457..14653
                     /gene="ispC"
                     /locus_tag="OQM83_001408"
     CDS             13457..14653
                     /gene="ispC"
                     /locus_tag="OQM83_001408"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCS1519703.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14842..15600
                     /gene="ispU"
                     /locus_tag="OQM83_001409"
                     /gene_synonym="uppS"
     CDS             14842..15600
                     /gene="ispU"
                     /locus_tag="OQM83_001409"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCS1519704.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15613..16470
                     /gene="cdsA"
                     /locus_tag="OQM83_001410"
     CDS             15613..16470
                     /gene="cdsA"
                     /locus_tag="OQM83_001410"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCS1519705.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16482..17834
                     /gene="rseP"
                     /locus_tag="OQM83_001411"
     CDS             16482..17834
                     /gene="rseP"
                     /locus_tag="OQM83_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCS1519706.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17864..20296
                     /gene="bamA"
                     /locus_tag="OQM83_001412"
     CDS             17864..20296
                     /gene="bamA"
                     /locus_tag="OQM83_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCS1519707.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20418..20903
                     /gene="skp"
                     /locus_tag="OQM83_001413"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20418..20903
                     /gene="skp"
                     /locus_tag="OQM83_001413"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCS1519708.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20907..21932
                     /gene="lpxD"
                     /locus_tag="OQM83_001414"
     CDS             20907..21932
                     /gene="lpxD"
                     /locus_tag="OQM83_001414"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCS1519709.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22037..22492
                     /gene="fabZ"
                     /locus_tag="OQM83_001415"
     CDS             22037..22492
                     /gene="fabZ"
                     /locus_tag="OQM83_001415"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCS1519710.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22496..23284
                     /gene="lpxA"
                     /locus_tag="OQM83_001416"
     CDS             22496..23284
                     /gene="lpxA"
                     /locus_tag="OQM83_001416"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCS1519711.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23284..24432
                     /gene="lpxB"
                     /locus_tag="OQM83_001417"
     CDS             23284..24432
                     /gene="lpxB"
                     /locus_tag="OQM83_001417"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCS1519712.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24429..25025
                     /gene="rnhB"
                     /locus_tag="OQM83_001418"
     CDS             24429..25025
                     /gene="rnhB"
                     /locus_tag="OQM83_001418"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCS1519713.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25062..28544
                     /gene="dnaE"
                     /locus_tag="OQM83_001419"
     CDS             25062..28544
                     /gene="dnaE"
                     /locus_tag="OQM83_001419"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCS1519714.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28557..29516
                     /gene="accA"
                     /locus_tag="OQM83_001420"
     CDS             28557..29516
                     /gene="accA"
                     /locus_tag="OQM83_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCS1519715.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7158 a         7117 c         8157 g         7106 t
ORIGIN      
        1 ttgaagatgc ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg
       61 aggcgtgggg tcagcatgag aaaggttcgc tcttttcgct agtggttgaa aatgcctggg
      121 aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac
      181 gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc
      241 ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg
      301 atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat ccggatgtcg
      361 agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt
      421 tattaagcct gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt
      481 tccctattga gtcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg
      541 aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc
      601 gttgccgcct actgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat
      661 accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt
      721 ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac
      781 agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac
      841 tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg
      901 agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg
      961 tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta
     1021 tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttcac
     1081 cgtttcagag ctctccgttc tgccaggggg gccagggcgg taatggcggc ggccagcaac
     1141 agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca
     1201 ccaacaacca tgttgttgat aacgcgacgg tgattaaagt ccagctgagc gatggtcgta
     1261 agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg atccaaatcc
     1321 agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg
     1381 attacaccgt agcgattggt aacccgtttg gtctgggcga gacggtaact tccgggattg
     1441 tctctgcgct ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc atccagaccg
     1501 atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga
     1561 tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta
     1621 tcccgagtaa catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac
     1681 gcggtgagct ggggattatg gggactgagc tgaactccga actggccaaa gcgatgaaag
     1741 ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag
     1801 cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccgatc agcagctttg
     1861 ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttac
     1921 tgcgcgacgg taagcaggtt aacgtgaacc tggaactgca gcagagcagc cagaatcagg
     1981 ttgattccag ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag
     2041 atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga
     2101 agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc
     2161 gtaaagtgct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca
     2221 ccatctacct gttaatgcag taatctccct caaccccttc ctgaatacgg gaaggggttc
     2281 tccttacaat ctgtgaactt cgccacaact ccatacatct tcatcatcct ttgggcattt
     2341 gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg
     2401 gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac catgcgcatc
     2461 atcgatacca atatcaacgt aatggatgcc cgcgggcgaa ttatcggcag cggcgatcgt
     2521 gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc
     2581 gatatcgatg acgcggtagc acgtcatctg cacggtgtgc ggcaggggat taatctaccg
     2641 ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc agagaatctg
     2701 cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg
     2761 atgcacctgt tggcgcagga tagccgtttg cgggaagaac tggtgatgaa cctgattcag
     2821 gctgaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat tgatctcaat
     2881 caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggcgt ggacagcgca
     2941 atggcggagt tacaacaact gcaaaatgcg ctgactacgc ccgagcgtaa taatctggtg
     3001 gcaattgttt cgctgaccga aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc
     3061 tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat gaaagagtac
     3121 ggccagctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg cagtattgcc
     3181 cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat gccagaaagt
     3241 cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt gcgtggcgac
     3301 tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga caataatggc
     3361 ttgctgcgac gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct ggctacgtca
     3421 aaggcgctgt ttattcatcg taatactctg gagtatcggc ttaatcgtat atcggaactg
     3481 accgggcttg atttgggtaa ttttgatgac aggttgctgc tgtatgtggc gttacaactg
     3541 gatgaagagc ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt cccctcagcc
     3601 cgaagggtag aggggaatgt ccgagttaac cgtcagttcg gcattattta cgcgttagtt
     3661 tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc aggtgacgta
     3721 agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat
     3781 caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct ttataaccct
     3841 gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac ttcacgctct
     3901 tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg ttgttggctt
     3961 cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga cttttcagat
     4021 tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt agcatttggc
     4081 atggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac ttctatcatg
     4141 gcgtaaaaat caaaaaatta cctgctttat tgtggtgata aaattcacgt tctacaccag
     4201 ttccagcatc gttacttccg ggcggcagtt cagacgcaaa ccatacaaac tgcccacgcc
     4261 acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt aacgtttatc
     4321 ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc cgtgagtatg
     4381 gccgcacagc atcagatccc agggttcatc acgcatgact tctttgctgt cgggattatg
     4441 tgccagcatc agtcttggca gatttgcttc gctggcggga ggtggtttgc attgtccggc
     4501 ccataagtca ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga tcaccgtagc
     4561 ctggttaaac aacaccgtga tgcccactga tttcaacgtc tcgccaatta agtgattttt
     4621 ttctgtacca acagggcgat cgtggttgcc gaagcaggca aacgtcggcg cacattcggc
     4681 aaggggggag aggacatcac taaacgccga aaaattcagc ggcatatcaa ataatacgta
     4741 atcgccgccc agtaatatca aatcgggctt ttgttctatg ccaagagcaa tcgcgtcaga
     4801 aatcaggctt aaaggaacaa aacgagagta atggagatcg gccagaaaaa gaattttgaa
     4861 tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa accagcctag
     4921 ctcccagtaa tgcatataac cgaaacctga gctcgtggct atcgtcgcgg cagtagcctg
     4981 caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg cagcgtcgtg
     5041 ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc
     5101 gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt atttgccatc
     5161 ttttgacggc agattacctg aaacaaccac agaccccgcc ggaacgcgac cgtagtggat
     5221 ttcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatggaaat
     5281 gacggaacct tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc caatgaagca
     5341 gttatcttca atgatggttg ggttagcctg cagcggttcc agcacgccgc cgatgcccac
     5401 gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt
     5461 atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac
     5521 ggtgttacgg gcaataaacg caccctgacg taccgccgct ggtggcacaa cgcggaagcc
     5581 ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta
     5641 gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc
     5701 ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc
     5761 accggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcgtt
     5821 ggctggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg
     5881 cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc
     5941 ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagtgcac gccggtcagc
     6001 ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcaccatg
     6061 aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggctggtc
     6121 gagcgcgata agctcaagga acgatttgcg gtcggtatgg gttggcaaaa acgtcacttc
     6181 ggtttcaaca gtaaagtgac gtagtttagc cggttgacga cgcggctgtg gtggctgcca
     6241 gctactttgt gtcagtacct gctccagacc aaaccgaata acctcatgac gatctgcgga
     6301 tagcgggcta ccatcgggtt ccagcacgat gaaggtatcc atcgccatac cgtcgcgagt
     6361 ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg cacagacggc
     6421 ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc
     6481 ctgcgggcta agcaatacca gcggtttgct taaatcatgc tgtaataaat ggcgggcatg
     6541 ccaggccagt tgatttgggc tatggcggac aaaatagtta gcacgacagc gtgaccaaat
     6601 ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagtg cctggagttg
     6661 gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt
     6721 ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc acagcgtttc
     6781 gttggtggcg caaatgtcag ccactgtcag gcataccaga tagtgcagac gattttccgt
     6841 ttgaacttct tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc
     6901 tgtcaccgac atcagcaagt gctggcgaac cagccaggca accagctgtg tttcgcgtga
     6961 gttcagcccg tggagttctg caaaatgcac tacatcctga gcaccgagaa tggagtggtc
     7021 gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga
     7081 cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt cttcactggc
     7141 aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa
     7201 cagatcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg cgccgagcac
     7261 gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac gcagaatgct
     7321 caaaaacagt tttcgtgctt ccggaatatt acacagcggt tgttgcagat ggcgacgggc
     7381 atggcgtaac tggcgcagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat
     7441 ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag
     7501 gtcgattagc gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc
     7561 gggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac
     7621 gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt tgccttcacc
     7681 actgtaattc agacgctgag cgacgctaag ttggcgatcg aataacaggc gattatcgta
     7741 acggctgacg accagatgca gggcaaagcg aatacgccac agtatatgca gacattcgtt
     7801 taattccgcc cgctccgctg aggttaagaa gccaaaccca accatttcat ccagcgatgt
     7861 tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca agccgccagg
     7921 gctacttttg atgtctggtt caagattgta gctggtgccg tggtaacgct gatggcgctg
     7981 gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa
     8041 aatatgtttt tgcagttcga ggaagagcgc aacatcgcca attaataagc gggattcgat
     8101 taaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt caagcgtgcg
     8161 cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt
     8221 ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag
     8281 tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt cggcaatctg
     8341 gctgaatccc gcttcaatcc ataatcgttg caggagctgg tcgataaact cggtgcgcgc
     8401 ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc aacgctggaa
     8461 agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc agacgcatgg
     8521 attttgcggt tgaccgggca gggtggggag agcggtgttt gcgtactgtt ctggaagggt
     8581 attcattgtg cgccacccat aagattaaat tatcgcatta aaaaagccgg cattcgccgg
     8641 cttcatctta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca
     8701 gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc
     8761 gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt
     8821 tgaccattgg ctcgatggtg aacgtcatcc caggtttcag tacgacgttg gtttcacggg
     8881 agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccgcaat
     8941 attcacgaac gacggagaag ccttctgctt cgataaactt ctgaatcgcc gcaccgattt
     9001 cgcgcaggtt aatgcctggt ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga
     9061 tgcggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat
     9121 cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca
     9181 gcagcttcgc atcgtccggg ataccgtggc acaccacttc attaatagag atgcaaacgg
     9241 atttcggata gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt
     9301 aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga
     9361 tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg
     9421 gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata
     9481 tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc
     9541 caaacgtgcc actgaacgtt ttctataata gaaaattcaa cgtctgatgc tgtacacagc
     9601 gccaacaatt attggtgtcc atgacgtatt tgtggtataa agcgcgccgg acttccgatc
     9661 catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg
     9721 tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata
     9781 accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca
     9841 aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca
     9901 tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca
     9961 acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg
    10021 gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct
    10081 tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca
    10141 tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgaa aagctgacca
    10201 agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta
    10261 tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca
    10321 ttgctatcaa agaagcaaac aacctgggta ttccggtatt tgctatcgtt gataccaact
    10381 ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga
    10441 ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt
    10501 cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat
    10561 agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg
    10621 accgggatga gcgagcgaag acaacgcatc tgcggcgcga aatatgaagg gggagagccc
    10681 ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt
    10741 ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga
    10801 aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg
    10861 caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa
    10921 gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa
    10981 aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt
    11041 tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg
    11101 caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt
    11161 agcgaaaatt ggtgagaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct
    11221 gggttcttat cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga
    11281 agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc
    11341 agaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg atatcgcgat
    11401 gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt
    11461 caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg
    11521 tcagctgctg aaagagcata acgctgaagt gactggcttc atccgcttcg aagtggggga
    11581 aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc
    11641 ttaattatcg aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc
    11701 agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat
    11761 ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa
    11821 cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat
    11881 gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact gggtattcag
    11941 gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt
    12001 atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca
    12061 atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg
    12121 aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt
    12181 gtggtgatcc tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc
    12241 ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg
    12301 tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc
    12361 gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac
    12421 cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta
    12481 atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt
    12541 aagattccgc gtaagtatcg cgggggcgta agtctggtta taaggcgtta ttgttgcagg
    12601 cagtttggtc acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg aggatggcga
    12661 gcactgctcg gggccaaaat ggcaaataaa atagcctaat aatccagacg attacccgta
    12721 atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac
    12781 tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta
    12841 cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt
    12901 gcttctccca gcctgctgga tggcattgtc gtggaatatt acggcacgcc gacgccgctg
    12961 cgtcagctgg caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat
    13021 cgttcaatgt ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg
    13081 aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa
    13141 gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg
    13201 cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat cagcgaagac
    13261 gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat caagaaaatt
    13321 gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct tgaacgacaa
    13381 aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt tattctgtct
    13441 caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct cgattggttg
    13501 cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg cgctggtggc
    13561 aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa ttctctcccc gctatgccgt
    13621 aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac agggtagccg
    13681 caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg aggatgttga
    13741 tcaggtgatg gcagccattg ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg
    13801 cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg gacgtctgtt
    13861 tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg aacataacgc
    13921 catttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg accttgagca
    13981 aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg agacgccatt
    14041 gcgcgatttg gcaacaatga cgcccgatca agcctgccgt catccgaact ggtcgatggg
    14101 acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat acattgaagc
    14161 gcgttggctg tttaacgcca gcgccagcca gatggaagtg ctgattcacc cgcagtcagt
    14221 gattcactca atggtgcgct atcaggacgg cagtgttctg gcgcagctgg gggaaccgga
    14281 tatgcgtacg ccaattgccc acaccatggc atggccgaat cgcgtgaact ctggcgtgaa
    14341 gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt atgatcgtta
    14401 tccatgcctg aaactggcga tggaggcgtt cgaacaaggt caggcagcga cgacagcatt
    14461 gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc gctttacgga
    14521 tatcgctgcg ttgaatttat ctgtactgga aaaaatggat atgcgcgaac cacaatgtgt
    14581 ggacgatgtg ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg
    14641 tctcgcaagc tgaggataat ccggctacag agagtcgcgc tatttgttag cgtagggctt
    14701 cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt caggtatgcc
    14761 gtggttttac acggcttttt tttgtatagg cttcagtatt cctgagtacc gtaaaccctg
    14821 tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga aaaattgcca
    14881 gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg ggcaaaaaag
    14941 caagggagga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg ccgggctgtc
    15001 tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag tagtgaaaac
    15061 tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat
    15121 agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg cgataccagt
    15181 cgctttaact cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac agctgggaat
    15241 accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat agtccaggga
    15301 gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa
    15361 gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt agtaattagg
    15421 actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta tgccgaactt
    15481 tactttacag atgttctctg gcctgatttc gatgaacaag actttgaagg ggcgttaaat
    15541 gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga aacagcctga
    15601 tggggggcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc
    15661 atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca tcgtaacgct ggtggtctgc
    15721 atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc gcagcgagta
    15781 tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt gccggaatat
    15841 caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgtt gggttggtgg
    15901 attgtcgcgc tattgctggt gctgttttac ccaggttccg cagcaatctg gcgtaactct
    15961 aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg gggcatgctg
    16021 gcgttacggg cctggcacta tgacgagaat cattacagtg gcgcaatatg gctgctctat
    16081 gtcatgatcc tggtatgggg cgctgactcc ggcgcatata tgtttggcaa attgtttggt
    16141 aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt tataggtgga
    16201 ctagcgaccg cagcggtaat ctcatggggt tatggcatgt gggcgaatct cgacgtcgct
    16261 cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct cggcgatctg
    16321 accgagagta tgtttaagcg tgaagcagga attaaggaca gcggtcattt aattccagga
    16381 cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt ctttgcttgc
    16441 ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt tttctctggg
    16501 atttggcttc gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa tttggtcatt
    16561 tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg
    16621 cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatggccctg attccgctgg
    16681 gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa ctccgccacc
    16741 atgccttcaa taataaatct gttggccaac gagcggcgat tattgccgca ggtccggttg
    16801 caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt gtgcctggcg
    16861 tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca caaattgcac
    16921 caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt
    16981 tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg ccatttggca
    17041 gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag cctgataaag
    17101 aagatccggt atcttcgctg gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg
    17161 aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc gacaggatcg
    17221 ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt gtccgggata
    17281 acccgggtaa atccttagcg ttagaaatcg aaaggcaggg gagtcccttg tctttgacat
    17341 taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt attgagccga
    17401 aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg ttcaacgcca
    17461 tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt atgctgggaa
    17521 aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct atcgccaagg
    17581 gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt gcgcttatta
    17641 gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg gggcatctgc
    17701 tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt caagactttt
    17761 gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt
    17821 tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga tgaaaaagtt
    17881 gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt
    17941 gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggcac tcctcagtat
    18001 gccggtgcgc acaggcgaca cggttaatga tgaagatatc agtaatacca ttcgcgctct
    18061 gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata cccttctggt
    18121 tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca aatcggtgaa
    18181 agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga
    18241 tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca gcgtcggtaa
    18301 atatagcgcc agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa
    18361 actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg ttggtaacca
    18421 tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag tgccgtggtg
    18481 gaacgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgacc ttgaaaccct
    18541 gcgcagctac tatctggatc gcggttatgc ccgtttcaac atcgactcta cccaggtcag
    18601 tctgacgcca gataaaaaag gtatttacgt cacggtgaac attaccgaag gcgatcagta
    18661 caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg aaattgagca
    18721 gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca agatggaaga
    18781 tgacatcaaa aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc
    18841 tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg
    18901 tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg
    18961 tcgcgaaatg cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg atcagggtaa
    19021 ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc aacgtgttcc
    19081 gggtagcccg gaccaggtag atgtagtcta caaggtgaaa gaacgtaaca ccggtagctt
    19141 caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg gcgtgcaaca
    19201 ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa acgattacca
    19261 gacctatgct gaattgtcgg taaccaaccc gtacttcact gtagatggtg taagcctcgg
    19321 tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg actataccaa
    19381 caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata actcgctgcg
    19441 tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg ttgcgatgtg
    19501 gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata acagcttcaa
    19561 aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc gtggttactt
    19621 cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg gatcggataa
    19681 cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg acgatcacaa
    19741 atgggttgtt ctggggcgta cccgctgggg ttatggtgat ggtttaggcg gcaaagagat
    19801 gccgttctac gagaacttct atgccggtgg ttccagcacc gtgcgtggct tccagtccaa
    19861 tacaattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg atccggacta
    19921 tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt
    19981 aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc cgtttattag
    20041 cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta ccgtttggga
    20101 tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc caagcaatat
    20161 ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt tggtgttctc
    20221 ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc agtttaacat
    20281 cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt
    20341 aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg tgcaaatggg
    20401 atggtaagga gtttattgtg aaaaagtggt tattagcggc aggtctcggt ttagcactgg
    20461 caacttctgc tcaggcggct gacaaaattg caatcgtcaa tatgggcagc ctgttccagc
    20521 aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca
    20581 gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg cagtccatga
    20641 aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc cagacttttg
    20701 ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa gaacgcggca
    20761 aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag gatatcgatc
    20821 tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac atcactgccg
    20881 acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat ttagcgcagc
    20941 agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc
    21001 aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt gagcatttag
    21061 gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc gcgaaaagtg
    21121 ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa attttagata
    21181 ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg acggcgaagc
    21241 tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga gtccggcgtt gaactgggcg
    21301 ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt
    21361 cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag aattgcctga
    21421 tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat cgtggtaact
    21481 gggtgaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct
    21541 gcacaaccat cgatcgcggc gcgctggatg acactgttat tggcaatggc gtgatcattg
    21601 ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg gttgccggtg
    21661 gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc ggagccagcg
    21721 taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg ggtatggtga
    21781 tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag
    21841 tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag cgtctgaaat
    21901 cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg ccaaacttta
    21961 cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt tcttatattt
    22021 tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag agattttaga
    22081 acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt ttgaagaagg
    22141 tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc agggccattt
    22201 ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac aggcaacagg
    22261 tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact acttcgctgg
    22321 tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga tcatggaagt
    22381 cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg
    22441 taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct gatacgtgat
    22501 tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt caattggcgc
    22561 gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcgaaattg gtgagggtac
    22621 cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg ataatgagat
    22681 ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac
    22741 ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac
    22801 agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga tcaacgcgca
    22861 cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg caacgctggc
    22921 gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag tccatcagtt
    22981 ctgcatcatt ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc
    23041 tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct
    23101 gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt ataagctgat
    23161 ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac tggcggaaac
    23221 atatccggaa gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg
    23281 ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac ctccggcgat
    23341 atcctggggg ccggtttaat ccgcgctctg aaagaacatg tgcccaacgc ccgctttgtt
    23401 ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cttggtacga aatggaagaa
    23461 ctggcggtaa tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt
    23521 gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg tattgatgcg
    23581 cccgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat
    23641 tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc
    23701 accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa atacaatgta
    23761 ccgtgccgct ttatcggtca taccatggct gatgccatgc cgttagatcc agataaaaat
    23821 gccgcccgtg atgtgctggg gatccctcac gatgcccact gcctggcgtt gctaccgggg
    23881 agccgtggtg cagaagttga aatgcttagt gccgatttcc tgaaaacggc ccagcttttg
    23941 cgccagacat atccggatct cgaaatcgtg gtgccactgg tgaatgccaa acgccgcgag
    24001 cagtttgaac gcatcaaagc tgaagtcgcg ccagaccttt cagttcattt gctggatggg
    24061 atgggccgtg aggcgatggt cgccagcgat gcggcgctac tggcgtcggg tacggcagcc
    24121 ctggagtgta tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa gccttttacc
    24181 ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa tctgctggcg
    24241 ggcagagagt tagttaaaga attattgcag gaagagtgtg agccgcaaaa actggctgcg
    24301 gcgctgttac cgctgttggc gaacgggaaa accagccacg cgatgcacga taccttccgt
    24361 gaactgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc cgttctggag
    24421 ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt gtggatgaag
    24481 tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc
    24541 gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt ctggcgctct
    24601 atgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa
    24661 tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc
    24721 tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc
    24781 ctgcgatggc tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc gcgtctatcc
    24841 tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc ccgcaatatg
    24901 gttttgccca acacaaaggg tacccaaccg cttttcatct ggaaaaactg gctgaacacg
    24961 gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt
    25021 cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg
    25081 tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa accgcaccgt
    25141 tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat ttcaccaacc
    25201 tttgtggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag cctatcgtcg
    25261 gggcagattt taacgtccag tgcgacctgc tgggtgatga gttaacccac ctgacggtac
    25321 tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc
    25381 gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag
    25441 ggttgatcct tctttccggc ggacgcatgg gcgacgtcgg acgcagtctt ttgcgtggta
    25501 acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt
    25561 ttctcgagct gatccgcacc ggcaggccgg acgaagaaag ctatctgcac gcggcggtgg
    25621 aactggcgga agcgcgcggt ttgcccgtcg tggcgaccaa cgacgtgcgc tttatcgaca
    25681 gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt accctcgacg
    25741 atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa gaggagatgt
    25801 gcgagctgtt tgccgacatc cccgaagctc ttgccaacac cgttgagatc gccaaacgtt
    25861 gtaacgtaac cgtgcgtctt ggtgaatact tcctgccgca gttcccgacc ggggacatga
    25921 gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt ctggcctttt
    25981 tattccctga cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa cgtctggaga
    26041 ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc gttatggaat
    26101 ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc tccggtgcgg
    26161 gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa tttgacctgc
    26221 tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac gttgacttct
    26281 gtatggagaa acgcgatcag gttatcgagc acgtagcgga catgtacggt cgtgatgcgg
    26341 tatcgcagat catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc gacgtaggcc
    26401 gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc gaaactgatc ccgcccgatc
    26461 cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa atctacgaag
    26521 cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta
    26581 acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc gattttgcgc
    26641 cgctttactg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa agcgacgttg
    26701 aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc atcatcaact
    26761 gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccgccg ctggatatcg
    26821 ctgcgatccc gctggatgat aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg
    26881 cggtattcca gcttgaatcg cgcggcatga aggacctgat caagcgtctg caacctgact
    26941 gcttcgaaga tatgatcgca ctggtggcac tgttccgccc cggcccgttg cagtcaggga
    27001 tggtggataa ctttatcgac cgtaaacatg gtcgcgaaga gatttcctat ccggacgtac
    27061 agtggcagca tgaaagcctg aaaccggtac tggagccaac ctacggcatc atcctgtatc
    27121 aggaacaggt catgcagatt gcgcaggtgc tttctggtta taccctcggt ggcgcggata
    27181 tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat ggctaagcag cgttctgtat
    27241 ttgctgaagg tgcagaaaag aacggaatca acgccgaact ggcgatgaaa atcttcgacc
    27301 tggtggagaa attcgcgggt tacggattta acaaatcgca ctctgcggcc tatgctttgg
    27361 tgtcatatca aacgttatgg ctgaaagcgc actatccggc ggagtttatg gcggcggtaa
    27421 tgaccgccga tatggacaac accgagaagg tggtgggcct ggtggatgag tgctggcgga
    27481 tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc cacgtcaacg
    27541 acgacggcga aatcgtgtat ggtattggcg ccatcaaagg ggtaggtgaa ggtccgattg
    27601 aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt gatctctgcg
    27661 cccgtaccga caccaaaaag ttaaaccggc gggtgctgga aaaactgatc atgtccgggg
    27721 cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt gatgcgttaa
    27781 aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc
    27841 tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg tggccggagc
    27901 aggtggtgtt agatggggaa cgtgaaacgt taggtctgta ccttacggga caccctatca
    27961 accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc
    28021 cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg cgggttatgg
    28081 tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc gggcggctgg
    28141 aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa aaagaccgca
    28201 tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt aaaatgaccg
    28261 ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctcgcggg cttgctatct
    28321 cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag tctctggaag
    28381 cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat gcacgcgcgc
    28441 ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta aacgatctcc
    28501 gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg aatactatga
    28561 gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa atcgattctc
    28621 tgactgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa gaagtgcatc
    28681 gtctgcgtga aaaaagcgta gaactgacac gtaaaatctt cgccgatctc ggtgcatggc
    28741 agattgcgca actggcacgc catccacagc gtccttatac cctggattac gttcgcctgg
    28801 catttgatga atttgacgaa ctggctggcg accgcgcgta tgcagacgat aaagctatcg
    28861 tcggtggtat cgcccgtctt gacggtcgtc cggtgatgat cattggtcat caaaaaggtc
    28921 gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa ggttaccgca
    28981 aagcactgcg tctgatgcaa atggctgaac gctttaagat gcctatcatc acctttatcg
    29041 acaccccggg ggcttatcct ggcgtgggcg cagaagagcg tggtcagtct gaagccattg
    29101 cacgcaacct gcgtgaaatg tctcgcctcg gcgtaccggt agtttgtacg gttatcggtg
    29161 aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat atgctgcaat
    29221 acagcaccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg
    29281 acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga
    29341 aactgatcga ctccatcatc ccggaaccgc tgggcggcgc tcaccgtaac ccggaagcga
    29401 tggcggtatc gttgaaagcg caactgctgg cggatctggc cgatctcgat gtgttaagca
    29461 ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggttac gcgtaattcg
    29521 caaaagttct gaaaaagg
//
LOCUS       DAJVQG010000035        28797 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJVQG010000035 DAJVQG010000000
VERSION     DAJVQG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.035"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OQM83_001421"
     CDS             complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OQM83_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1519716.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="OQM83_001422"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="OQM83_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCS1519717.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="OQM83_001423"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="OQM83_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCS1519718.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="OQM83_001424"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="OQM83_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCS1519719.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="OQM83_001425"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="OQM83_001425"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCS1519720.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="OQM83_001426"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="OQM83_001426"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCS1519721.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="OQM83_001427"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="OQM83_001427"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCS1519722.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="OQM83_001428"
     CDS             complement(4604..5326)
                     /locus_tag="OQM83_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1519723.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="OQM83_001429"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="OQM83_001429"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1519724.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="OQM83_001430"
     CDS             6526..6744
                     /locus_tag="OQM83_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCS1519725.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="OQM83_001431"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="OQM83_001431"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCS1519726.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="OQM83_001432"
     CDS             complement(7478..7678)
                     /locus_tag="OQM83_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCS1519727.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="OQM83_001433"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="OQM83_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCS1519728.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OQM83_001434"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OQM83_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCS1519729.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="OQM83_001435"
     CDS             10175..12088
                     /locus_tag="OQM83_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1519730.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="OQM83_001436"
     CDS             12088..13110
                     /locus_tag="OQM83_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1519731.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="OQM83_001437"
     CDS             13104..13322
                     /locus_tag="OQM83_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCS1519732.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="OQM83_001438"
     CDS             13376..14245
                     /locus_tag="OQM83_001438"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCS1519733.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="OQM83_001439"
     CDS             complement(14300..14704)
                     /locus_tag="OQM83_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCS1519734.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="OQM83_001440"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="OQM83_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCS1519735.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15689..17779
                     /locus_tag="OQM83_001441"
     CDS             15689..17779
                     /locus_tag="OQM83_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCS1519736.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD/dapC"
                     /locus_tag="OQM83_001442"
     CDS             complement(17846..19066)
                     /gene="argD/dapC"
                     /locus_tag="OQM83_001442"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCS1519737.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="OQM83_001443"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="OQM83_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCS1519738.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="OQM83_001444"
     CDS             complement(19747..20349)
                     /locus_tag="OQM83_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCS1519739.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20339..20506)
                     /locus_tag="OQM83_001445"
     CDS             complement(20339..20506)
                     /locus_tag="OQM83_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCS1519740.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="OQM83_001446"
                     /gene_synonym="rotA"
     CDS             complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="OQM83_001446"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCS1519741.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21454..22635
                     /gene="tsgA"
                     /locus_tag="OQM83_001447"
     CDS             21454..22635
                     /gene="tsgA"
                     /locus_tag="OQM83_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCS1519742.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22897..25440
                     /gene="nirB"
                     /locus_tag="OQM83_001448"
     CDS             22897..25440
                     /gene="nirB"
                     /locus_tag="OQM83_001448"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCS1519743.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25437..25763
                     /gene="nirD"
                     /locus_tag="OQM83_001449"
     CDS             25437..25763
                     /gene="nirD"
                     /locus_tag="OQM83_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCS1519744.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25889..26695
                     /gene="nirC"
                     /locus_tag="OQM83_001450"
     CDS             25889..26695
                     /gene="nirC"
                     /locus_tag="OQM83_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCS1519745.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26714..28087
                     /gene="cobA"
                     /locus_tag="OQM83_001451"
     CDS             26714..28087
                     /gene="cobA"
                     /locus_tag="OQM83_001451"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCS1519746.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28334..28501
                     /locus_tag="OQM83_001452"
     CDS             28334..28501
                     /locus_tag="OQM83_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCS1519747.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         6995 a         7710 c         7219 g         6873 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccaaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggca ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagaat ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt caccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag
    20581 cgcgggcaag attgcccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac
    20641 taccggtttt gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat
    20701 cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg
    20761 ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct
    20821 gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc
    20881 ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc
    20941 gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct
    21001 gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag
    21061 ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc
    21121 tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa
    21181 catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc
    21241 ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac
    21301 gatcactttt tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc
    21361 aataaaacat ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg
    21421 ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc
    21481 acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg
    21541 gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc
    21601 accttcctca acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc
    21661 gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt
    21721 ttgatgttca gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc
    21781 agcggcatta ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag
    21841 cgcggttccc gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca
    21901 atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc
    21961 gggctggtgt atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc
    22021 aaacatgcac caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg
    22081 tttctctctg ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg
    22141 cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac
    22201 ttctggatgt catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat
    22261 ttgcaacgca ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac
    22321 accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg
    22381 atctatacca ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg
    22441 gttaactttg tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc
    22501 ccgattgttg aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct
    22561 gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc
    22621 ctgacctctc attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc
    22681 ttcccccgct acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa
    22741 acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca
    22801 cttaaggagt atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt
    22861 aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt
    22921 atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg
    22981 gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac
    23041 ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc
    23101 tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag
    23161 gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca
    23221 accggttcct acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc
    23281 tatcgcacca ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caaacgcggt
    23341 gccgttgttg gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt
    23401 attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg
    23461 ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa
    23521 aacacccttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac
    23581 ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag
    23641 ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc
    23701 tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt
    23761 gtatttggtc tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc
    23821 ggtagcgaaa acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta
    23881 gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac
    23941 ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg
    24001 ctcggtgcgg tactggtggg cgataccagc gactacggta acctgctgca actggtgctg
    24061 aacgctatcg aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc
    24121 ggcaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc
    24181 gacgtcacta aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg
    24241 ctgaaagctg aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag
    24301 gtactgaacg cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac
    24361 tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc
    24421 gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc
    24481 ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg
    24541 caggattcta acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc
    24601 ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg
    24661 cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca
    24721 cagaaagacg atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt
    24781 catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc
    24841 tacggtgttg gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc
    24901 cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct
    24961 cagggtaaag acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt
    25021 aacggcggca tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg
    25081 ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg
    25141 cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc
    25201 attgacgaca aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa
    25261 gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa
    25321 cacttcatca acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag
    25381 caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga
    25441 gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc
    25501 tgttaggtga cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga
    25561 tcagcaacat tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac
    25621 accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg
    25681 gcttgtgcat ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg
    25741 gcgtggtgca gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca
    25801 tggtcctgta atgaccttcg gtatattgca gaacattttt taaacttttt gttttatttt
    25861 ttgtttttat tttttaaagg ataatcaaat gtttacagac actattaata agtgtgcggc
    25921 taatgctgcg cgcattgcac gcctgtcggc aaataacccg ctcggctttt gggtcagctc
    25981 cgccatggcg ggcgcatatg tgggtcttgg gatcatcctg attttcacgc tcggtaattt
    26041 gctcgacccg tccgtacgtc cgctggtgat gggcgcgacc tttggtatcg ccttaacgct
    26101 ggtgattatc gccggttctg aactgttcac cggacacgcc atgttcctca cctttggggt
    26161 aaaagcgggc agcatcagcc acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg
    26221 taacctggtc ggttccgtct tcgttgccat gctctatagc tggggcggcg gtagcctgct
    26281 gccggtagat accagcatcg ttcactccgt cgcgctggct aaaaccactg caccggcaat
    26341 ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta tctggatggc
    26401 gctgcgcact gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc tggcatttat
    26461 cgcgtccggc tacgagcact ccatcgctaa catgacgctg ttcgccctct cctggttcgg
    26521 caaccacagc gaagcctaca cgctggcggg tattggtcat aacctgctgt gggtgacgct
    26581 gggtaatact ttatcgggtg ccgtattcat gggattgggt tattggtatg ctacgccgaa
    26641 agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa acggctgccg gttaattact
    26701 aaggggtttt tacgtggatc atttgcctat attttgccaa ttacgcgatc gcgactgtct
    26761 gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc
    26821 tcgcttaacg gtgaatgcat tagcgtttat tccacagttc accgcatggg cagatgcagg
    26881 catgttaacc ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct gctggctggc
    26941 gattgcagcg acggatgatg acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg
    27001 tcgcatcttc tgtaacgtag tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat
    27061 tattgaccgc tcaccgctaa tggtagcggt ctcctctggc ggcacctctc cggttctggc
    27121 gcgcctgttg cgcgaaaaac tcgaatcact gctgccgttg catctgggcc aggtggcgaa
    27181 atatgccggg caattacgcg ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg
    27241 ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg caaacaacga
    27301 tcagaaagcc attactgaaa cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg
    27361 tgaagtggtg ctggttggtg caggtccggg cgatgccggg ctgctgacgc tgaaaggact
    27421 gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt ctggtttctg acgatattat
    27481 gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg
    27541 tgtaccgcag gaagagatta accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt
    27601 ggtgcggctg aaaggtggcg atccgtttat ttttggccgt ggtggcgaag agctggaaac
    27661 actgtgcaac gcgggtattc cgttctcggt ggttccgggt attaccgcag cttctggttg
    27721 ctctgcctat tcgggtattc cactcacgca tcgcgattat gcccagagcg tacgcttaat
    27781 taccggacac ttaaaaaccg gtggcgagct ggactgggaa aacctggcgg cagaaaaaca
    27841 gacgctggtg ttctatatgg gattgaatca ggccgcgact attcagcaaa agctgattga
    27901 acacggtatg cctggcgaaa tgccggtggc aattgtcgaa aacggaacgg cagtcacgca
    27961 gcgcgtgatt gacggtacgc tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc
    28021 atcgctaatt attattggtc gggttgttgg cctgcgcgat aaactgaact ggttctccaa
    28081 ccattaattt aacccgggcc agagaattct ggccttctta atgactcttt ttatatattc
    28141 agcaaataaa acataacccc ttaataaata ttatctgcta atgggttata ttcaccgtta
    28201 cagcgactta caaaattaag tcattcgctc actgacgcca attaaatatt cctgtatcgt
    28261 tcttaacgag cagtctgctt atattcatat aatcaatgaa tattaattaa tatataatac
    28321 atagggaatg taaatgaaca aatttattaa agttgcactg gtaggtgcag tactggctac
    28381 gttaactgca tgtactggtc atattgaaaa ccgtgataag aactgctctt acgactacct
    28441 gctgcacccg gcaatttcta tttctaaaat cattggcggt tgcggtccta ctgcacagta
    28501 agtctctgac agaaatcggc taacaccggt ttaattgccc gacgccctct ggattttccg
    28561 agggcgtatt tgtttctatg tccttcttgc ccccgcccgt aaaaatattt tcactttaaa
    28621 ttcaatttga taactacatc cattagccac agcgcgatcc tgtgcgaaat tttgtgatct
    28681 tcctccacat tacataacat catatgttgt tatattcatc atgcattgtc atgttacctt
    28741 ttaaatgact gcaaactctc ccctacaacg tattggacaa gaaaaaggta tcgctat
//
LOCUS       DAJVQG010000036        28662 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJVQG010000036 DAJVQG010000000
VERSION     DAJVQG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28662
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.036"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..849)
                     /gene="melB"
                     /locus_tag="OQM83_001453"
     CDS             complement(<1..849)
                     /gene="melB"
                     /locus_tag="OQM83_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCS1519748.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTL"
     gene            complement(952..2307)
                     /gene="melA"
                     /locus_tag="OQM83_001454"
     CDS             complement(952..2307)
                     /gene="melA"
                     /locus_tag="OQM83_001454"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCS1519749.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYLHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2590..3498
                     /gene="melR"
                     /locus_tag="OQM83_001455"
     CDS             2590..3498
                     /gene="melR"
                     /locus_tag="OQM83_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCS1519750.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3697..5964
                     /gene="adiA"
                     /locus_tag="OQM83_001456"
     CDS             3697..5964
                     /gene="adiA"
                     /locus_tag="OQM83_001456"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCS1519751.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            6289..7050
                     /gene="adiY"
                     /locus_tag="OQM83_001457"
     CDS             6289..7050
                     /gene="adiY"
                     /locus_tag="OQM83_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCS1519752.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            7187..8524
                     /gene="adiC"
                     /locus_tag="OQM83_001458"
     CDS             7187..8524
                     /gene="adiC"
                     /locus_tag="OQM83_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCS1519753.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            8628..10271
                     /gene="eptA"
                     /locus_tag="OQM83_001459"
     CDS             8628..10271
                     /gene="eptA"
                     /locus_tag="OQM83_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCS1519754.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            10268..10936
                     /gene="basR"
                     /locus_tag="OQM83_001460"
     CDS             10268..10936
                     /gene="basR"
                     /locus_tag="OQM83_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCS1519755.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            10937..12037
                     /gene="pmrB"
                     /locus_tag="OQM83_001461"
     CDS             10937..12037
                     /gene="pmrB"
                     /locus_tag="OQM83_001461"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCS1519756.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISIFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(12013..12102)
                     /gene="pmrR"
                     /locus_tag="OQM83_001462"
     CDS             complement(12013..12102)
                     /gene="pmrR"
                     /locus_tag="OQM83_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCS1519757.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(12214..13716)
                     /gene="proP"
                     /locus_tag="OQM83_001463"
     CDS             complement(12214..13716)
                     /gene="proP"
                     /locus_tag="OQM83_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCS1519758.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(13980..14846)
                     /locus_tag="OQM83_001464"
     CDS             complement(13980..14846)
                     /locus_tag="OQM83_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCS1519759.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(14843..17071)
                     /gene="crfC"
                     /locus_tag="OQM83_001465"
     CDS             complement(14843..17071)
                     /gene="crfC"
                     /locus_tag="OQM83_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCS1519760.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGVHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            17484..17819
                     /locus_tag="OQM83_001466"
     CDS             17484..17819
                     /locus_tag="OQM83_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCS1519761.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            17977..18420
                     /gene="yjdN"
                     /locus_tag="OQM83_001467"
                     /pseudo
     CDS             17977..18420
                     /gene="yjdN"
                     /locus_tag="OQM83_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            18553..19341
                     /gene="phnC"
                     /locus_tag="OQM83_001468"
     CDS             18553..19341
                     /gene="phnC"
                     /locus_tag="OQM83_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCS1519762.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVALNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            19366..20382
                     /gene="phnD"
                     /locus_tag="OQM83_001469"
     CDS             19366..20382
                     /gene="phnD"
                     /locus_tag="OQM83_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1519763.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            20508..21287
                     /gene="phnE"
                     /locus_tag="OQM83_001470"
     CDS             20508..21287
                     /gene="phnE"
                     /locus_tag="OQM83_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCS1519764.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            21308..22033
                     /gene="phnF"
                     /locus_tag="OQM83_001471"
     CDS             21308..22033
                     /gene="phnF"
                     /locus_tag="OQM83_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCS1519765.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            22034..22486
                     /gene="phnG"
                     /locus_tag="OQM83_001472"
     CDS             22034..22486
                     /gene="phnG"
                     /locus_tag="OQM83_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCS1519766.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            22483..23067
                     /gene="phnH"
                     /locus_tag="OQM83_001473"
     CDS             22483..23067
                     /gene="phnH"
                     /locus_tag="OQM83_001473"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCS1519767.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            23067..24131
                     /gene="phnI"
                     /locus_tag="OQM83_001474"
     CDS             23067..24131
                     /gene="phnI"
                     /locus_tag="OQM83_001474"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCS1519768.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            24124..24969
                     /gene="phnJ"
                     /locus_tag="OQM83_001475"
     CDS             24124..24969
                     /gene="phnJ"
                     /locus_tag="OQM83_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCS1519769.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            24966..25724
                     /gene="phnK"
                     /locus_tag="OQM83_001476"
     CDS             24966..25724
                     /gene="phnK"
                     /locus_tag="OQM83_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCS1519770.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            25835..26515
                     /gene="phnL"
                     /locus_tag="OQM83_001477"
     CDS             25835..26515
                     /gene="phnL"
                     /locus_tag="OQM83_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCS1519771.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            26512..27648
                     /gene="phnM"
                     /locus_tag="OQM83_001478"
     CDS             26512..27648
                     /gene="phnM"
                     /locus_tag="OQM83_001478"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCS1519772.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            27648..28205
                     /gene="phnN"
                     /locus_tag="OQM83_001479"
     CDS             27648..28205
                     /gene="phnN"
                     /locus_tag="OQM83_001479"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCS1519773.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            28192..28626
                     /gene="phnO"
                     /locus_tag="OQM83_001480"
     CDS             28192..28626
                     /gene="phnO"
                     /locus_tag="OQM83_001480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCS1519774.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
BASE COUNT         6937 a         7956 c         7398 g         6371 t
ORIGIN      
        1 taacgtcacc aggttagcag ctcccgcata cgacagataa taggggaaca aatccgcatc
       61 accgataaca tatgagaaat aatagatagc aaatccggta ataatgttgc tggctacatt
      121 ataagcaaga gccataccca agaggcatga aagctgatcg tttttataaa ttagcgcaac
      181 gatggcttta agtgtcagat ggcttccttc agcagacggt tgattgtctg acgaaaagac
      241 ttcatgcaca ttgcgcagag tgatgatggt tgaaacaata aaaaaggcga tcagtaccag
      301 tgtgaacatc tgaaagccaa atccccgatc gccaccgccg acataattaa caaatggtag
      361 cgtcacacct gccgtaacaa agcccgccag actggcaaaa aaacgcggat aaggaaccag
      421 ttgttcacgc tcacgtttat cgagcgtgat ggttggaacc agcgaccaga agggaatatc
      481 cataatggtg taagtcatgc cccagaggat gtaggtcacg caaacaaaga caatctgagt
      541 agtaccttca aacagatgcg cactaaagag gagaaataag attacagagt ttgccaacgt
      601 accgatcagg atccagggtt tgaacttacc ccatcgcgat cgcgtagcat ttacaatcca
      661 tcccataatc ggatcgttaa tagcatccca gatcctcgcc accagaaaca aagtaccgac
      721 caaacccaca gacagcccaa cgacatcggt gtagtaatac atgaggtaca tatacacaat
      781 gccgatcgca aaatccttcc cgaacgctcc aaatccataa ctgagttttg tagtcattga
      841 aatgctcata gggtatcggg tcgctgttac caacgcccgc cttctgttat tggtatcgtc
      901 agaggtgtgc gccggaataa atcccccggc gcagtagcgt ttagtcgcgt tttaacggtg
      961 caaccagcct ggcagccagt cgccgtgggc ggcaatcagg tcgtcaacaa gagcatatat
     1021 ttcgtcaatg cccagcacgg cggcagtatg cgggtccatc atcgcggcgt ggtaaacacg
     1081 gtcgcgattt tccgtaagaa tagcttcggt cagcagcgtc tgtacattga tgttggtttg
     1141 catcagggcg gccagatgcg aaggtagcgt accgacttta gtcggctgaa tgccattagc
     1201 atcaaccaga caggctactt ccacgcaaca tccttgtggc aggttatcaa tcaaaccatc
     1261 gttacggacg ttgccgtaaa tcacactcgg ctcgccagtc cagatagcgt tcatgattgt
     1321 gctggcatat tctcgtgacg gtttaatatc aatccgggag gcgtttttat actcctccag
     1381 ctctttatgc cagttcgcca gctgctcgac gcagcgtttc gggtactcat ccagcggtac
     1441 tttataacgc tcaatcaaat cctcacgacc tggcttaata aaccacggcg tgtattcagc
     1501 aaaatgttct gacgattccg taacgaagta gcccagcttt ttgaacattt cgtagcgcac
     1561 aatattctgg cagcgagtat taccatgaat atttggcttc ggtgcctgcc ctgcttcata
     1621 agccgccagc agttccgggt agagattcac gtaactgccg tcggcggttt tacgctccag
     1681 ttccaggtaa aacgccatat ggttgatacc agcgcaacgg taacgcagcg tggacggatc
     1741 gatattgagg tcacgcgcca gctcttccgc cgttccctgc accgaatggc acagccctac
     1801 ctgtttgata tgcagatagc gggcatacat cgcccaggta ttcatcgcca ttgggttaac
     1861 gtagttgagc atggtggcat cggggcagac ttccgtcatg tcctcgcaaa tttgccacag
     1921 atgcggaatg gtacgtagcg cgcgcataat accgcccggc cccaacgtat cggcaatggt
     1981 ttgttccaga ccatgccgct tacagacctc gaaatcagtc accgtgcaag gttcataacc
     2041 gccaatctga aatgccacca cgacaaaatc ggcatcctgt aaggcttctt tctgttgggt
     2101 gtggcaggtg attttgccgc tggcccctgc tgaatccatc agcttacgca ccacaatatg
     2161 cgactcttcc aggcgggtgg gatcaatgtc catcagggca atatgcgccg ttttcagcgc
     2221 ctcgcgatgg aacacatcac caagaatatt tttaacgaaa atcgtcgaac cagcgccgat
     2281 aaatgtaatt ttgggtgcag tcatcatgca gatctcctgg cttgcttgaa taacttcatc
     2341 atggcaggcg aatctgcaga aaacctccgg cttccaggtt gagcattccc ataaactcag
     2401 atcctgcgtg aagcagcagt aaatctgagt ttatggtaat aagttacctg aaaagcagag
     2461 ttttcggcct catgatgctc ggacgctccc ctcgcctctc tgggaagagg gtgaaaaccg
     2521 tgctcccact cgcagtcatc ctccctcact cctgccataa ttctgatatt ccaggaaaga
     2581 gagccatcca tgaatacaga tacgtttatg tgcagcagcg acgaaaaaca gacccgcagc
     2641 ccgctgtcgc tgtattcgga ataccagcgg atggagattg agtttcgcgc accgcatatc
     2701 atgcccacca gccactggca tggtcaagtc gaagtgaatg tgcctttcga tggcgatgtg
     2761 gaatacctga tcaacaatga aaaagtgaat atcaatcagg gtaatatcac gctgttctgg
     2821 gcctgtacac cgcaccaact aacagatacc ggaacctgtc agagcatggc gatttttaat
     2881 ctgccgatgc atctgtttct ctcctggccg ctggataaag acctgattaa ccacgtcact
     2941 cacggcatgg tgatcaaatc actggccaca cagcaactta gcccgtttga agtgcgccgc
     3001 tggcagcagg aattaaacag tccgaacgag caaattcgcc agctcgccat tgatgaaatt
     3061 ggcctgatgc tcaagcgatt tagcctctct ggctgggaac cgattctggt caataaaacc
     3121 tcgcgcacac acaaaaacag cgtctcgcgc catgcgcaat tttatgttag ccagatgctg
     3181 ggctttattg ccgaaaacta tgatcaggcg ctgaccatca acgatgtggc tgagcacgtc
     3241 aaacttaacg ccaactatgc aatggggata ttccagcggg tcatgcaatt gacgatgaaa
     3301 cagtacatta ccgcgatgcg catcaaccac gttcgcgcgt tactgagcga taccgataaa
     3361 agtattctcg atattgccct gacggcaggc tttcgttcaa gtagtcgttt ttacagcacg
     3421 ttcggcaaat atgtcggcat gtcgccgcaa caataccgca aacttagcca acagcgccgc
     3481 cagacgtttc ccggctaaag acgatatcag tatcagccaa aaaaatagtt tagccgtcga
     3541 taataagcag tgaaaataat ttaaaataat cacataacgc attgtttata aaaataaata
     3601 tttcaaaaat gtttgttttt cacgcgcttt acagcccgaa aaggccggaa gatacttgcc
     3661 cgcaacgaag attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc
     3721 gagtttctcc atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta
     3781 agccagcaaa atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc
     3841 tcttcaaacg aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa
     3901 catcaaaacg tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc
     3961 ttcctgttgg gcgatcggga aaaagccctc gccgcaatgg atcgcgacct gctggagctt
     4021 gtcgatgaat tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgctgtt
     4081 gccgcgatga cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa
     4141 tatagcgaca tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt
     4201 accaaaacac ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc
     4261 gatatgggca tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc
     4321 gaaagcgaaa aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc
     4381 ggtacttccg gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg
     4441 gtcgttgacc gtaactgcca taaatccatc gaacaaggct tgatgctgac aggcgcgaaa
     4501 ccggtctaca tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag
     4561 gaaatgcaac ctgaaacctt gcagaagaaa atcagtgaaa gcccgctgac taaagacaaa
     4621 gccgggcaaa aaccgtctta ctgcgtggtg accaactgca cctatgacgg cgtgtgttat
     4681 aacgctaaag aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa
     4741 gcctggtacg gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc
     4801 gaacctggcg atcacaacgg tcctaccgtt ttcgccaccc actccaccca caaactgctg
     4861 aatgcgcttt cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc
     4921 tcccgcttca accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc
     4981 gcatccaacg acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacgcag
     5041 gaagtgatcg acgaagcggt tgatttccgt caggcgatgg cgcggctgta taaagagttc
     5101 accgctgacg gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa
     5161 accggcaaaa cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac
     5221 tgctgggtaa tgcatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg
     5281 agtatgctcg acccgattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa
     5341 ctggaagaaa ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt
     5401 gtgcctaccc gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt
     5461 gggaaatggg gaactctggt taacaccctt tgctccttca aacgccacta tgacgccaac
     5521 acaccgctgg cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac
     5581 atggggattc acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc
     5641 gcacggttga acgaagccta ttccggcctg tcggtggcgg aaatcacccc gcgtgaagcg
     5701 tacaacgcga ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc
     5761 atcgcggcaa actcagttat cccatatccg ccaggaatcc cgatgctgct gtctggtgaa
     5821 aacttcggcg ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac
     5881 caccatttcc ctggatttga acacgaaact gaagggactg aaattattga cggtatttac
     5941 cacgttatgt gtgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac
     6001 attacgccgc ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca
     6061 aacaaaaatg tcatactttt tgcgcggccc caccccgcgc ttttttttgc ctgttattta
     6121 tcctgtaaaa aaatatgtac atgagaaatt actataaaaa tttgtaatat tagtaaaact
     6181 cgttattttt atgcatgttt atattcatca tacaattata taaccatttc ccggtatcgc
     6241 tttgctttag cgagaactgg tcttttattc gcactcagga gtacatgtat gaggatttgc
     6301 agcgaccaac cttgtattgt tttattgact gaaaaagatg tctggataag ggtgaatggg
     6361 aaagaaccta ttagccttaa agctaaccat atggcgttat taaattgtga aaataatatt
     6421 atcgacgtct cctctcttaa caacactttg gttgctcata ttagtcacga catcatcaaa
     6481 gattacctcc ggtttctgaa taaagatctc tcgcaaatac cagtatggca acgtagcgct
     6541 acgcccatac tcaccctgcc atgcctgacg ccagacgtct ttcgcgttgc cgcgcaacac
     6601 agcatgatgc ccgcagaaac tgagtcagaa aaggaacgaa cacgcgcatt attattcacg
     6661 gtgctatccc gttttctcga cagtaaaaaa ttcctttcat taatgatgta tatgttacgt
     6721 aattgtgtaa gtgacagcgt ttatcaaatt attgaaagcg atattcacaa agactggaat
     6781 cttagtatgg tagccagttg tttatgtctt agcccaagtc tgttaaagaa aaagctgaaa
     6841 agcgaaaaca ccagttatag ccaaataatc accacctgcc gcatgcgtta tgccgtaaat
     6901 gaattaatga tggacggtaa aaatatctct caggtatcac agtcctgcgg ctacaacagt
     6961 acgtcgtact ttatttctgt ctttaaagac ttctacggta tgacgccgct gcattatgtt
     7021 agtcagcaca gagaacgaac tgtcgcctga tttttaacct taacgaagag ctatattaat
     7081 aacggcatca gcgataaccc ggtcgataat aattcaacta tcgaatgcag gcgtatgata
     7141 tgacgtaatt tattgtcacg aagctcgcct tcgcaggagt ttaattatgt cttcggatgc
     7201 tgatgctcac aaagtgggct taatccccgt caccctgatg gtgtcgggga atattatggg
     7261 gtcaggtgtt tttctgttac ctgcaaacct ggcctccact ggcgggattg ccatttatgg
     7321 atggttggtg acgattatcg gtgcgctggg gctctcgatg gtatacgcca aaatgtcgtt
     7381 cctcgatcca agtcctggtg gttcttacgc ttacgcccgc cgctgctttg gcccgtttct
     7441 cggttatcaa accaacgtcc tctactggct ggcctgctgg atcggcaata tcgctatggt
     7501 ggtcattggc gtaggatatt taagttactt cttcccgatt ctgaaagatc cattggtatt
     7561 aaccatcacc tgcgtcgtgg tgctgtggat cttcgtcctg ctgaacattg tcggtccgaa
     7621 aatgatcacc cgtgtgcagg ccgttgccac cgtgctggcg ctgatcccca tcgtcgggat
     7681 tgccgtattt ggctggttct ggttccgtgg tgaaacctat atggcggcat ggaacgtcag
     7741 cggcctgggc accttcggtg cgattcaaag tacccttaac gttacgctgt ggtcgttcat
     7801 cggtgtggaa agtgcctccg ttgccgcagg tgtggtgaaa aacccgaaac gcaatgtccc
     7861 tatcgccacc attggtgggg tattgattgc cgccgtttgc tatgtgctct ccaccaccgc
     7921 gattatgggg atgatcccta atgccgcact gcgcgtttct gcctcgccat tcggtgatgc
     7981 cgcacggatg gcgttgggtg acaccgctgg ggccattgtt tccttctgcg cagctgcggg
     8041 ttgcttaggt tcactgggcg gctggacgtt gctggcgggt caaacggcga aagccgctgc
     8101 cgatgacgga ctgttcccac cgatttttgc ccgtgtaaat aaagcgggta cgccagtggc
     8161 ggggctgatt atcgtcggta ttttgatgac catcttccag ctcagtagca tttcgccaaa
     8221 cgcaaccaaa gagttcggtc tggtatcttc cgtgtcggtc atctttacac tggtgccata
     8281 tctttacacc tgtgcggcgt tactgctgct cggccacggt cactttggta aagcacgccc
     8341 ggcatatctg gctgttacta ccattgcctt cctctactgc atctgggccg tggtggggtc
     8401 cggggcgaaa gaggttatgt ggtcatttgt caccctgatg gtcatcaccg ccatgtatgc
     8461 gctgaattac aaccggctac ataaaaaccc gtatccctta gatgcaccaa taagcaaaga
     8521 ttaattcccc ttaatccagc aaacataaaa gccaacctta agaacttaag gttggcttaa
     8581 ttttgctttg cgagcatatg cgcactttgt tcgatggaaa caccgtgatg ttgaagcgcc
     8641 tactaaaaag cccctctttg aatttactcg cctggctatt gttggccgct ttttatatct
     8701 ctatctgcct gaatattgcc ttttttaaac aggtgttgca ggcgctgccg ctggattcgc
     8761 tgcataacgt actggttttc ttgtcgatgc cggtcgtcgc tttcagcgtg attaatattg
     8821 tcctgacact aagctctttc ttatggctta atcgaccact ggcctgcctg tttattctgg
     8881 ttggcgcggc tgcacaatat ttcataatga cttacggcat cgtcatcgac cgctcgatga
     8941 ttgccaatat tattgatacc actccagcag aaagttatgc gctgatgaca ccgcaaatgt
     9001 tattaacgct gggattcagc ggcgtgcttg ctgcgctgat tgcctgctgg ctaaaaatca
     9061 aacctgccac ctcgcgtctg cgcagtgttc ttttccgtgg agccaatatt ctggtttctg
     9121 tactgctgat tttgctggtc gccgcactgt tttataaaga ctacgcctcg ttgttccgca
     9181 ataacaaaga gctggtgaaa tccttaagcc cctctaacag cattgttgcc agctggtcat
     9241 ggtactccca tcagcgactg gcaaatctac cgctggtgcg aattggtgaa gacgcccacc
     9301 gcaacccgtt aatgcagaac gaaaaacgta aaaatttgac catcctgatt gtcggcgaaa
     9361 cctcgcgggc ggagaacttc tccctcaacg gctacccgcg tgaaactaac ccgcggctgg
     9421 cgaaagataa cgtggtctat ttccctaata ccgcatcttg cggcacggca acggcagttt
     9481 cagtaccgtg catgttctcg gatatgccgc gtgagcatta caaagaagag ctggcacagc
     9541 accaggaagg cgtgctggat atcattcagc aagcgggcat caacgtgctg tggaatgaca
     9601 acgatggcgg ctgtaaaggt gcctgcgacc gcgtgcctca ccagaacgtc accgcgctga
     9661 atctgcctgg tcagtgcatc aacggcgaat gctatgacga agtgctgttc cacgggctgg
     9721 aagagtacat caataacctg cagggtgatg gcgtgattgt cttacacacc atcggcagcc
     9781 acggtccgac ctattacaac cgctatccgc cgcagttcag gaaatttacc ccaacctgcg
     9841 acaccaatga gatccagacc tgtaccaaag agcaactggt gaacacttac gacaacacgc
     9901 tggtttacgt cgactatatt gttgataaag cgattaatct gctgaaagaa catcaggata
     9961 aatttaccac cagcctggtt tatctttctg accacggtga atcgttaggt gaaaatggca
    10021 tctatctgca cggtctgcct tatgccatcg ccccggatag ccaaaaacag gtgccgatgc
    10081 tgctgtggct gtcggaggat tatcaaaaac ggtatcaggt tgaccagaac tgcctgcaaa
    10141 aacaggcgca aacgcaacac tattcacaag acaatttatt ctccacgcta ttgggattaa
    10201 ctggcgttga gacgaagtat taccaggctg cggatgatgt tctgcaaact tgcaggagag
    10261 tgagtgaatg aaaattctga ttgttgaaga cgatacgctg ttattgcagg gactgattct
    10321 ggcggcgcaa accgaaggct acacgtgcga tggcgtaaca accgcgcgga tggcggaaca
    10381 aagccttgag gccggtcatt acagcctggt ggtactggat ttagggttac ccgacgaaga
    10441 tggactgcat tttctcgccc gtatccggca gaaaaaatac accctgccgg tactgatcct
    10501 caccgctcgc gatacgctga ccgacaaaat cgccgggctg gatgtcggtg ccgatgacta
    10561 tctggtgaag ccttttgcgc tggaagagtt acacgcccgt atccgcgccc tgctacgacg
    10621 ccataataat cagggcgaaa gtgagctgat tgttggcaat ctgacgctga atatgggtcg
    10681 ccgtcaggta tggatgggcg gtgaagagtt gattctgacg cccaaagaat atgctctgct
    10741 gtcacggtta atgctcaaag caggcagtcc ggtacatcgg gaaattctct acaacgacat
    10801 ctataactgg gacaatgaac cctcgaccaa caccctggaa gtgcatatcc acaatctgcg
    10861 cgacaaagtg ggcaaagccc gtatccgcac cgtgcgcggc tttggctata tgctggtcgc
    10921 gaatgaggaa aactaattga atctgatgcg ttttctgcgc cgaccaatat cgctgcgcca
    10981 acggctgata ttgaccatcg gggccatttt gttggtgttt gagctgatca gcatcttctg
    11041 gctatggcat gaaagtaccg agcagattca gctgtttgag caggcgctac gcgacaatcg
    11101 caacaacgat cgccacatca tgcgtgagat ccgcgaggcg gtcgccagcc tgattgtccc
    11161 cggcgtcttt atggtcagcc tgacgctatt tatctgctat caggcggtac gccgcatcac
    11221 ccgcccgctg gcggagctgc aaaaagagct ggaagcgcgc accgccgaca acttaacgcc
    11281 cattgccatt cacagcgcca ccctcgaaat cgaagcggtg gtttcggcgt tgaacgatct
    11341 ggtcagtcgc ctgacgagca cgctggataa cgaaaggtta tttaccgctg acgtcgcgca
    11401 cgaactgcga acgccactgg cgggggtgcg tttgcatctg gaactgctgg cgaaaacgca
    11461 tcacattgat gtagcaccgt tagtggcacg gcttgatcag atgatggaga gcgtctccca
    11521 gctgctgcaa ctggcgcgtg ccggacagtc attttcttcc ggtaattatc aacatgtaaa
    11581 actgctggaa gatgtcattc tcccctcgta tgacgaactc agtaccatgc tcgaccagcg
    11641 acagcaaacc ctgctattgc cagagagcgc cgccgacatc accgttcagg gtgatgcaac
    11701 cctgctgcgg atgttattgc gaaacctggt agaaaacgcc catcgttaca gcccacaagg
    11761 cagcaacatt atgattaagc tgcaagaaga tggcggagcg gtcatggccg ttgaagatga
    11821 aggaccgggt attgatgaga gtaaatgcgg ggagttgagt aaagcgtttg tacgtatgga
    11881 cagccgttat ggcgggattg gtctggggtt aagtattgtc agccgcatta cacagttgca
    11941 tcacgggcag tttttcctgc aaaaccggca agagacttcc ggcacgcggg cctgggtacg
    12001 gctgaagaaa gatcagtacg tagcaaacca gatataaaga aagctgctga ccaccagcac
    12061 actgaacacg gtagttaaac tttcataaac acggtttttc attgcactct cctccgttaa
    12121 cctggaggag agtatgcgcg tcagagatta agtgaacctt aagagttcaa ttggcctgat
    12181 aagacagcgt cacatcaggc catccgtttc agcttattca tcaattcgcg gatgttgctg
    12241 caccaggcgg gtacgtttcg cctgcaaatc ggcaatctcg tggtcaatat catcgatttt
    12301 ctgctcgata ttatcgtaat gctcgacgag aatttccttc gcttcctgta tatctgacgc
    12361 cgccggtgtc gcacctttca acggacgatt tgccgtctct ttcatggtta cgccggtgat
    12421 taaaccaatc accgccacta ccatcaggta ataggcaggc atcatcagat tctgcgagct
    12481 ttcgaccagc caggccgcca gtgttggcgt cagaccggca accagcaccg aaatattaaa
    12541 tgccgccgcc agcgcgctgt aacggatatg cgtcgggaac atcgctggca aggtagaagc
    12601 cataacgccc gtaaagcagt taaggatcac cgccagcatc agtaacccgg caaaaatcag
    12661 gccgatgacg ttactgttaa tcagaataaa cgccgggatc gccaacacaa acagggcaac
    12721 actaccaagt agcacaaacg gacgacggcc aaaacggtca ctcagcaagc ccatcaccgg
    12781 ctggacaaac agcataccga tcataatggc gataataatc agcaccccgt ggtcttcgga
    12841 gtaatgcagg ttatgcgaca aataactcgg catataggtc agcaacatgt agtaagtcac
    12901 gttggtggca attaccagac caatacatgt caacaggctg cgccagtatt tagtggcaat
    12961 ctctttaaac gagactttcg ggccatcctg caaaccttcg cggtcgccct gttccagttt
    13021 atcaacatgc tgctggaacg ccggagtttc ttccagcgca tggcgcaggt aaagcccgat
    13081 aatccctaac ggcagagcaa taaagaacgg aatacgccag ccccagtcga ggaagttcgc
    13141 ttcgccgaca atggtcgaaa ttaacaccac cacgcccgca cccagcacaa acccggcaat
    13201 agaaccgaag tccagccagc tgcccataaa gccacgttta cggtcagggg agtattccgc
    13261 aacaaatatc gacgccccgg tatattcacc gccgaccgag aaaccttgtg ccatcttaca
    13321 gatcaacagc agaatcggtg cccaaatacc aatcgtgtcg taggacggta ttaagccaat
    13381 acagaacgta ctgatcgaca taatcacaat agtgatagcg aggatcttct ggcgaccata
    13441 tttatcgccc aacataccaa agaagagccc gccaagcggt cgaatcagaa agggaacgga
    13501 gaaagtggca agtgcagcaa ccatctgcac gctggggtca gcccccggga aaaaaacttt
    13561 acctaatgcg taagcaacaa aaccataaac accaaaatcg aaccattcca ttgcattacc
    13621 cagtgatgct gcggtaatgg ctttacgcag tttaccgtca tcaataatgg tgacatcacg
    13681 aagggtaatc ggttttactt ttttcctttt cagcatagct ttcctcgcag agttggccca
    13741 tttccgcaaa cgccagaaaa atcagcgcga cggccgcaaa aggggagcgg gaattgcagg
    13801 atgcaatctc tgtaacaagc gtagcgggtt tgcttacacc ctccggttaa ggaatgtaca
    13861 atcaaaccag ttgacgcctg aattgggcag ttaatgacct ttttacccta ccagtgttta
    13921 aatttgtgat caacttcaca catggatttc gtttaggact cattgatgta actgattatt
    13981 tacgccaaat ttacagagaa gatttcctcg accattgccc ccaccaaacg ctgcgctgaa
    14041 cagagtcgtg gcatccccag cgcgactgtt tgccagcgtt gggtcactga ccagcaaacg
    14101 ggctgtttat ctgccgtgga ccactttccc agccaactta aaaaatcagc atgctcccct
    14161 cccgaagagg caaaccactg atggtaatag tgacgcgtgg tcggtgccgc attgatactc
    14221 tctgccagat gttgtgtcgc tttctcgcgc aaatcaccca gtaacaacac caccgcttgc
    14281 gggcttccca gacggcaggc atcgccaaat gcaccccagc gcaactcctc cagaacgata
    14341 aaacagcgcc caggcaacga ccaggcgtta tacgccccag cacgccagcg ggtaaaaatt
    14401 tgctcgctct gatcacctgc ctgcaccagt aggcctcgtt gcgttaacgc cttttccttc
    14461 cagcgacaac gggctgaaaa ccactcatac aaggcggcaa tttgctgggg gagctccggg
    14521 aaagcagagt gctggcggtg atccattttt tgcagcagcg ccagatgctg cgcgatctgc
    14581 gtcagcgcca gatgaccggg atccagcatc gacgccagtt tttccaccat tgtcagtcgc
    14641 aggctaagcg gcgcatttaa catgccgccg ttcgtccatg tctgaagctg cacacgcgaa
    14701 agaagttcct gcatcagtcg ttcacgaaac tgctgctgat gggcttgtgg aaggcgggca
    14761 tcatcgccct gcgccagatc cactaaaaag cggggataaa ccgactccag cacgcgaccg
    14821 gggccgtcca gtaacgtctt ggtcaatatc gttctgccgt gaaaagtgtt tgaatatcat
    14881 cgcgtaacag ctgggcgtcg gtgtaaatcc agccgtgagt catcacagtc tgctgcaatt
    14941 gctgctgcat cagcctgacc accgattcat tttgttgacg cagagccagg ctttcgcgta
    15001 aacgcgtctg taattccgtc aaacatgaag cgaactcagc gcaaaaagta ttcatgcctg
    15061 ccgtaacaga ttcatcgacc tgctctgcca gaactttagc catttgttgg caataaagat
    15121 cgacttctgc gcttaaagct cgttgcaaca cactgtaatc aaccgtctct gtcggggatt
    15181 tctcatttcc ccgtccccag tcgggctgat tcaaccagcg cgaaaaagtc tcacgcacaa
    15241 cgcctaaacg cgtgctctgc tcgtccgttg catcctggcg cgaaatgact tcactgaaca
    15301 gctggcgagt gttgacgtgg ggaactactc cgtgaaaaac aggaaaatga accccaggac
    15361 gaaaccctga ctcgctcaat tccattttga cttgttgctc aatgggccga ataacatcgg
    15421 ttaacacccg gcaaagggtg gattcaagct cggcaaaacg cagcgtaaag tcgcgactga
    15481 tggtgttctg cgccgtctgt aacagtgtct cacagcaggt acgaatctcg cttaacggct
    15541 ccgaatcatc ctgaaacaag gcggctaact gcgcattcag cgcatcttgc tgttgacgca
    15601 gaaagtggtt ggcggaggtc agggccagct cgatttcatg tttaatctcg ccgctcacct
    15661 gcgcctgatt gagttgcaat agctgcaaac tttcttcgac ctgatggata ttttgccgca
    15721 attgttcaca agcgacgttt aacccgtgcg cacgaaaatc caggtattcc cgcgcctgct
    15781 gcgcgtaatt caacagttta tgcgcagcag atcgcagagc atacaacgag gcgttagcgt
    15841 aagcggcatg aagcaacgcc tgaattggct gggcgaacag cgaatcttcc cacaactgat
    15901 cggcagcatg acgaatatgt tcgaggtccg ccagatcggc atgacgccag cgcctgccga
    15961 gcgcggcatg ggcaaaatct tccacccagc gttgttgctc tggcgctggt aacttaccgt
    16021 tgttggctaa ctcatggcgc gctcgatttg ccaagtagcc ccacatcgac gacaccggaa
    16081 atatctgctg tggcgtaata cagcctttca tcagcgtccc ggaaatcagt gcccgcacct
    16141 ggtcggcgtc gtcactgtta cgatcctgtt gatcgaactt attgaccagc acatacagtg
    16201 gcaccgattg ccccaccgcc aaaatcgcct cacggacctc ttcatcggag atcgatttca
    16261 gttgcgtata atccagcacc gccagtaccg ccgaggcgcg tgccagctgc tggttaagca
    16321 ttttttgcag atgcggttgc ccggcttcat ttggcccggg ggtatccagt aacgtcaact
    16381 gaccgggata actctccagc cccgccagat ggacaaactc cacttcaatc acgggaatat
    16441 gctcaatggc ggcgtaagca gaaaaaggaa aatcgacgtc cagcgccttc gccagtcgca
    16501 ctaaatcatt caaacttttc agacaatgaa aaataggctg ggcacccaga taatattttt
    16561 cgaaagcgac gccattttcg atccgctgca taagcgcacg catatcttta tctatttcca
    16621 gcacatcggt cagatgctta atatcgcaat cacgcaggcg ctgttgtaat tgttgaatta
    16681 aacaatcgat tggcgcgaca tgtgaaaaat gcagtaccgg ttccttttgc ccgggcgtat
    16741 ggcgaataag cgtcggcagc gcagtcattg ggcgattacg attaggcaga acctccgtac
    16801 caacaatggc attaatggtg gttgatttcc ctgctttcat agtaccgaca atcgccagca
    16861 ccatttccag tcgggaaatt ttactcaact cattattcag catcgcgtga cgttcggcga
    16921 tattaggctg actccagggt aaagccagtt gtggcgcgtc gtctccgggt acagagagag
    16981 gcattttttc cagtaactgc aactgttggc gagaaagctg taacaggcgt tcagcctcct
    17041 gacttaactc atacagggtc tgtgtgtaca tagaaaattc ttccttaaag caaattttgt
    17101 tacttttatt tagccagatt gtttttgagt tctgtttgag ttctgttttt ggcttttata
    17161 attactgcaa gaaataattt tatatttagt gtgttgtttt ttatcagaat aaataacgtc
    17221 ttctgatgcg tttaaaacgt cagaaagata aaaatatcat gtgaataaaa aaaagaacaa
    17281 gtagagcatt aacattatct taaataataa atagaggcaa aaagattatt ttctttttgc
    17341 gtttcctttc aaatgaaaac gatcgtcgtc taaaatcagc agtacccccg acaaactcag
    17401 ggattttgtg tataattgcg gcctttttcg gcaatctgcc gttttttggc gcttttgccc
    17461 tgctgacttt tgaggaaatc cacatgtcat taccacactg cccaaaatgc aactccgaat
    17521 acacttacga agataacggc atgtacatct gcccggaatg tgcctacgaa tggaacgacg
    17581 cagaacctgc acaggaaagc gacgagctga tcgttaaaga tgctaacggc aatctgctgg
    17641 ctgacggcga cagcgttacc atcattaaag atctgaaggt gaaaggtagc tcttcgatgc
    17701 tgaaaattgg caccaaagtg aaaaacatcc gcctggttga aggcgaccat aacatcgatt
    17761 gcaaaatcga cggttttggt ccgatgaaac tgaaatctga gtttgtgaaa aagaactgat
    17821 tgtattgtga tcggtaagcc ggataaggcg ctcgcgccgc atccggcaac gatgccgact
    17881 gcctgatgcg acgcttgcgc gtcttatcag gcagcctctc atctgtataa atttcgaact
    17941 acacttaatt ggcttctctt aactgaggtc accatcatgc cgttaagtcc ctacctctct
    18001 tttgccggta actgtgccga cgcaattgct tattatcaac gtacgttggg cgctgaactg
    18061 ctctataaaa tcagcttcgg cgaaatgcca aaatcagcgc aggacagcgc cgagaactgc
    18121 ccttccggaa tgcaatttcc cgataccgcc atcgcccacg ccaatgtgcg cattgccgga
    18181 agcgacatca tgatgagcga tgccatgccg tcaggaaaag ccagctactc cggctttacg
    18241 ctggtgctcg attcgcaaca ggtcgaagaa ggaaaacgct ggtttgacaa tcttgccgct
    18301 aacggaaaaa tcgaaatggc ctggcaggaa actttctagg cgcatggctt tggcaaagtc
    18361 accgataaat ttggcgtacc gtggatgatt aatgtcgtca aacaacaacc aacgcaataa
    18421 cccgccggga ggcccgccct cccgcactgt catcgaattc ccgttaactc ttcatctgtt
    18481 agtcactttt aattaaccaa atcgtcacaa taatccgcca cgatggagcc acttttttag
    18541 ggaggctgca tcatgcaaac gattatccgt gtcgagaagc tcgccaaaac cttcaatcag
    18601 catcaggcgc tgcatgcggt tgctctgaac attcatcacg gtgaaatggt ggctctgctt
    18661 gggccgtcgg gttccggcaa atccaccctt ttacgtcact taagcggttt gattaccggc
    18721 gataaatccg ccggcagcca tatcgagctg ctgggccgca cagtccagcg cgaaggccgt
    18781 ctggcgcgcg atatccgcaa aagccgcgcc aacaccggct acatcttcca acaattcaac
    18841 ctggtgaacc gcctgagcgt actggagaac gtgctgattg gcgcgctcgg cagcacgccg
    18901 ttctggcgca cctgttttag ctggtttacc cgcgagcaga aacaacgcgc gttacaggcg
    18961 ctgacccgcg ttggcatggt gcattttgcc catcaacgcg tttccaccct ctccggcgga
    19021 cagcagcagc gtgtggcgat tgcccgcgcg ctgatgcagc aggcgaaggt gattctggcc
    19081 gatgaaccca tcgcctcgct ggacccggaa tccgcccgca tcgtgatgga caccctgcgc
    19141 gacatcaatc agaacgacgg catcaccgtg gtcgtcacgc tgcatcaggt ggattacgcc
    19201 ctgcgctact gcgaacgcat cgtcgccctg cgccaggggc acgttttcta cgacggcagc
    19261 agccaacagt ttgataacga acgttttgac catctctacc gcagcattaa tcgcatcgaa
    19321 gagaacgcga aagctgcctg acatccccat cattgaggaa aacgaatgaa cgctaagata
    19381 attgcctcgc tggccttcac cagcatgttc agcctcagca ccctgttaag cccggcacac
    19441 gccgaagagc aggaaaaggc gctgaatttc ggcattattt caacggaatc acagcaaaac
    19501 ctgaaaccgc aatggacgcc attcttacag gatatggaga agaagctggg cgtgaaggtg
    19561 aacgccttct ttgccccaga ctacgcaggc attatccagg gaatgcgctt caataaagtg
    19621 gatatcgcct ggtacggcaa cctgtcggca atggaagcgg tggatcgcgc caacggccag
    19681 gtcttcgccc agacggtcgc ggcggatgga tcgccaggtt actggagcgt gttgatcgtc
    19741 aacaaagata gtccgatcaa caacctgaac gatctgctgg cgaagcggaa agatctcacc
    19801 ttcggcaatg gcgatcctaa ctccacctct ggcttcctcg tccccggtta ctacgtcttt
    19861 gccaaaaaca atatctccgc cagcgacttt aagcgcaccg tcaacgccgg gcatgaaacc
    19921 aacgcgctgg ccgtcgccaa caagcaggtg gatgttgcca ccaacaacac cgaaaacctc
    19981 gacaagctga aaacctccgc gccagagaag ctgaaagaac tgaaggtgat ctggaagtcg
    20041 ccgctgatcc caggcgatcc gatcgtctgg cgcaagaatc tctccgaaac caccaaagac
    20101 aagatctacg acttctttat gaactacgga aaaacgccgg aagagaaagc ggtgctggaa
    20161 cgcctgggct gggcaccgtt tcgcgcctcc agcgacctgc aactggtgcc gattcgccag
    20221 ctcgcactgt ttaaagagat tcagggcgta aaaagcaata acggactgaa tgagcaggac
    20281 aagctggcga aaaccaccga gattcaggcg cagctggatg acctggaccg cctgaacaac
    20341 gcgttaagcg cgatgagttc ggtgagtaaa gcggtgcagt aatgtgctcg atcggtcccc
    20401 tctcccttga gggagagggt tagggtgagg gggaaatgcg cacgctgatg ccctcacccc
    20461 ggccctctcc cacagggaga gggagaaaac attaaacgga gccaaacatg caaaccatca
    20521 ccatcgcccc acccaagcgc agctggttct cgcttctgag ctgggccatt gttctcgccg
    20581 tgttggtcgt ctcgtggcag ggcgcggaaa tggccccgct tacgctgatc aaagacggcg
    20641 gcaacatggc gaccttcgcc gccgacttct tcccgcccga tttcagccag tggcaggatt
    20701 acctcaccga aatggccgtc acgctgcaaa tcgccgtctg gggcaccgcg ctggcagtgg
    20761 ttctctccat ccccttcggc ctgatgagcg ccgaaaacct ggtgccgtgg tgggtttacc
    20821 agcccgttcg ccgcctgatg gacgcctgcc gcgccattaa cgaaatggtc ttcgccatgc
    20881 tgttcgtggt cgccgtcggt ctcggaccgt tcgctggcgt gctggcgcta tttattcaca
    20941 ccaccggcgt gctctcgaaa ctgctttccg aagcggtgga agcgattgaa cccggcccgg
    21001 tggaaggcat tcgcgccacc ggcgccaaca agctggaaga gatcctttac ggcgtgctgc
    21061 cacaggtaat gccgctgctg atctcctact ccctctatcg cttcgaatcc aacgtccgct
    21121 cggcgaccgt cgtcggcatg gtcggtgcag gcgggatcgg cgtcaccctg tgggaagcga
    21181 ttcgcggttt ccagttccaa caaacctgcg ccctgatggt gcttatcatc gtcacggtca
    21241 gcctgctgga tttcctctct caacggttgc gtaagcactt tatctgataa gtgaggcatt
    21301 gatatctatg cacttgtcta cacatccgac cagctaccca acacgctatc aggagatagc
    21361 cgcaaaactt gagcaggagc ttcgtcaaca ctatcgctgc ggcgactatc ttcccgccga
    21421 gcagcaactg gcggcgcgct ttgaggtaaa ccgccacacc ctgcgccgcg ccatcgacca
    21481 actggtggaa aaaggctggg tacagcgccg tcagggcatc ggcgtgctgg tgctgatgcg
    21541 cccgttcgat tacccgctca acgcccaggc gcgttttagc cagaacctgc tggaccaggg
    21601 cagccatccc accagcgaaa aactgctgtc ggtattgcgc cccgcgtccg gtcacgttgc
    21661 tgacgcgctg gggattaccg agggtgagaa cgttatccat ttgcgcaccc tgcgtcgcgt
    21721 caacggcgtc gcgctctgtt taatcgacca ctactttgcg gacctcaccc tctggccgac
    21781 actgcaacgt ttcgacagcg gttcgctgca cgattttctg cgcgagcaaa ccggaattgc
    21841 gctgcgccgc agccagacgc ggatcagcgc ccgccgcgcc caggccaaag agtgccagcg
    21901 ccttgaaatc ccgaatatgt cgccgctgct gtgcgtgcgc acccttaacc accgtgacgg
    21961 tgaaagcagc ccggcggagt actccgtcag cctgacgcgc gccgacatga ttgaattcac
    22021 tatggagcac tgaatgcacg cagataccgc gacccgccag cactggatgt ccgtactggc
    22081 gcacagccaa ccggctgaac tggcagcccg cctgaacgcg ctgaacatca ccgccgacta
    22141 tgaagtgatc cgcactgccg aaactggcct ggtgcagatt caggcgcgaa tgggcggtac
    22201 cggcgaacgc ttttttgccg gcgacgccac gctgacccgt gccgccgtac gcctgactga
    22261 cggcaccctc ggctacagct gggtgctggg gcgtgataaa cagcacgccg aacgctgcgc
    22321 gctgattgat gcgctgatgc agcaatctcg ccactttcaa aacttatcag aaacccttat
    22381 tgccccgctg gacgctgacc gtatggcacg cattgccgca cgccaggccg aagtgaacgc
    22441 cagccgggtc gacttcttta cgatggttcg cggagacaac gcatgaccct ggaaaccgct
    22501 tttatgcttc ccgtgcagga tgcccagcac agttttcgtc gcctgttaaa ggccatgagc
    22561 gagccgggcg tgattgtcgc cctgtatcag ctcaaacgcg gctggcagcc gctgaatatc
    22621 gccaccacca gcgtgctgct gacgctggcc gataacgaca cgccggtgtg gcttgccacg
    22681 ccattaagta acgatatcgt caatcagagc ctgcgttttc ataccaacgc gccgctggtc
    22741 agccagccgg aacaggcgac cttcgcggtg acggatgagg cgatttccag cgaacagctc
    22801 aacgcccttt ccaccgacac cgccgttgcg ccggaagcgg gcgcgacgct gattttacag
    22861 gtcgccagcc tgagcggcgg acgcatgttg cgtctcaccg gcgcgggcat tgccgaagaa
    22921 cgaatgatcg ctccgcagct gccagagtgc attctgcacg aactcaccga gcgcccgcac
    22981 ccgttcccgc tcggcatcga cctaatcctg acctgcggcg agcgcctgct ggctatcccg
    23041 cgaaccactc atgtggaggt gtgctgatgt acgttgccgt gaaagggggc gaaaaggcga
    23101 tcgacgccgc ccacgccctg caagagagcc gacgccgggg cgataccgat ttgcccgaac
    23161 tgagcgtcgc ccagattgaa cagcagctta acctcgcggt ggatcgcgtg atgacagaag
    23221 gcggcattgc cgaccgcgaa ctggcggcgc tggcgctgaa acaggccagc ggcgataacg
    23281 tcgaagcgat tttcctgcta cgcgcctacc gcaccacgct gacgaaactg gcggtaagcg
    23341 agccgctcga caccacggag atgcgtctcg aacggcgtat ttcagcggtt tataaagata
    23401 ttcccggcgg ccagctgctt ggcccaacct acgactacac ccatcgcctg ctcgatttca
    23461 ctctgctggc aaacggcgaa gcgccgacgc tgaccaccgc cgacagcgaa caacagccgt
    23521 cgccgcacgt tttcagcctg ctggcgcgtc aggggctggc gaagtttgaa gaggatagcg
    23581 gagcacagcc ggatgacatc acccgcacgc cgccggttta cccctgttca cgttcttccc
    23641 gtttgcagca gttaatgcgc ggcgacgaag gctatttgct ggcgctggct tactccaccc
    23701 agcgcggtta cgggcgcaac cacccgttcg cgggcgagat ccgcagtggt tacatcgacg
    23761 tgtcgattgt gccggaagag ctgggatttg cggtaaacgt cggcgaactg ctgatgaccg
    23821 agtgtgaaat ggtcaacggt tttatcgacc cgccggatga gccgccgcac ttcacgcgcg
    23881 gctacgggct ggtattcggc atgagcgagc gcaaagcgat ggcaatggcg ctggttgacc
    23941 gtgcgttgca agccccggaa tacggcgagc acgcgacagg cccggcgcag gatgaagagt
    24001 tcgtgctggc gcatgccgac aacgtcgaag ccgcaggctt tgtctcgcac ctcaaactcc
    24061 cgcactacgt cgatttccag gccgaactgg agctactcaa acgtctgcaa caggagcaga
    24121 accatggcta atctgagcgg ctacaacttt gcctacctcg acgagcagac caaacgcatg
    24181 atccgccgcg ccatcttaaa agcggtggcg atccccggtt atcaggtacc gtttggcggg
    24241 cgcgagatgc cgatgccgta cggctgggga actggtggta tccagctcac cgccagcgtg
    24301 attggcgaaa gcgacgtgtt gaaggtgatt gaccagggtg cggatgacac caccaacgcc
    24361 gtgtcgattc gcaacttctt taagcgcgtg accggggtaa acaccactga atgtacggac
    24421 gatgcgacgc ttatccagac gcgtcaccgc atccccgaaa cgccgctgac cgaagatcag
    24481 atcattatct tccaggtgcc aatcccggaa ccgctgcgct ttatcgagcc gcgcgaaacg
    24541 gagacccgca ctatgcacgc gctggaggag tacggcgtga tgcaggtgaa actgtatgaa
    24601 gatatcgccc gcttcggtca tatcgccacc acctatgctt atccggtgaa ggtgaacggg
    24661 cgctacgtga tggacccgtc gccgatcccg aaattcgata acccgaaaat ggacatgatg
    24721 cccgccctgc agctgttcgg cgcagggcgc gagaagcgca tctatgcggt gccgccgttt
    24781 acccgcgtgg aaagtctcga tttcgacgat cacccgttca ccgttcagca gtgggatgag
    24841 ccatgcgcca tctgcggatc gacccacagc tatcttgatg aagtggtgct ggatgacgcc
    24901 ggaaaccgca tgtttgtctg ctccgatacc gattattgcc gccaacagag cgaggcgaaa
    24961 agccaatgaa tcaaccgtta ctttcggtca ataacctgac ccacctttac gcgccgggca
    25021 aaggctttag cgatgtctct tttgatttat ggccggggga agtgctgggc attgtcgggg
    25081 aatccggctc cgggaagacc acgctgctga agtcgatctc cgcgcgtctg acgccgcagc
    25141 agggggagat tcactatgaa aaccgttccc tctatgcaat gagcgaggcc gaccgccgtc
    25201 gcctgctgcg caccgaatgg ggcgtggtgc atcagcatcc gctcgacggc ctgcgccgcc
    25261 aggtgtcggc aggcggcaat atcggcgagc ggctgatggc gaccggggcg cgacattacg
    25321 gcgatattcg cgctaccgcg cagaagtggc tggaagaggt ggagattccc gccaaccgta
    25381 tcgacgacct gccgaccacc ttttccggcg gtatgcagca gcgtttgcag attgcccgca
    25441 acctggtgac gcatccgaag ctggtgttta tggatgaacc gaccggcggg ctggatgtgt
    25501 cggtgcaggc ccgcctgctc gacctgctgc gcggactggt ggtggagctg aacctcgcgg
    25561 tagtgattgt cacccacgat ttaggcgtcg cccgcctgct ggcggaccgt ttgctggtga
    25621 tgaagcaggg gcaagtggtg gagagtgggt taaccgaccg cgtgctcgac gacccgcatc
    25681 atccgtatac acagctgctg gtgtcatcgg ttttgcagaa ttgagccggt gccggatgcg
    25741 gcgtaaacgc cttatccggc ctacaaatgc gctccccgta ggtcggataa gacgcgtcag
    25801 cgtcgcatcc gacacccgaa ccacgaggcg aaaaatgatt aacgtacaaa acgtcagtaa
    25861 aaccttcatc ctgcaccagc aaaacggcgt gcgcctgccc gtcctcaacc gcgcctcgct
    25921 catcgtcaac gcgggcgaat gcgtggtgct ccacggccat tccggcagcg gaaaatcaac
    25981 cctgctacgc tcgctgtacg ccaactatct gcccgacgaa gggcaaatcc agatcaaaca
    26041 cggtgacgaa tgggtcgatc tggtcaccgc gccagcgcgc aaagtggtgg agatccgcaa
    26101 aaccaccgtt ggctgggtga gccagtttct gcgcgtcatc ccgcgtattt cggcgctgga
    26161 agtggtgatg cagccgctac tcgataccgg cgtaccgcgt gaagcctgcg ccgctaaagc
    26221 cgcgcgtctg ctcacccgcc tgaacgtgcc ggaacgcctg tggcatctgg cgccatcgac
    26281 cttctccggc ggcgaacagc agcgcgtcaa catcgcccgc ggctttatcg tcgactaccc
    26341 cattctgctg ctcgacgaac ctaccgcctc gctggacgcc aaaaacagcg ccgccgtagt
    26401 cgagctgatt cacgaagcca aagcccgtgg cgcggccatc gtcggcatct tccacgacga
    26461 agccgtacgt aatgacgttg ccgaccgcct gcacccaatg gaagcctctt catgattatc
    26521 aataacgtca aattggtgct ggaaaacgaa gtggtacacg gttcactgga ggtgcaggac
    26581 ggcgaaatcc gcgcctttgc cgaaagccag agccgcctgc cggaggcgat ggacggcgaa
    26641 ggcggctggc tgctgccggg gctgattgag ctgcataccg ataatctgga taaattcttc
    26701 accccgcgcc cgaaagttga ctggcctgcc cactcggcga tgagcagcca cgacgcgctg
    26761 atggtggcga gcggcatcac caccgtactg gatgccgtgg caattggcga cgtgcgcgac
    26821 ggcggcgatc ggctggtgaa tctggagaag atgatcaacg ccatcgaaga gacgcagaaa
    26881 cgcggcgtca accgcgccga gcaccgtctg catctgcgct gcgaactgcc gcatcacacc
    26941 acgctgccgc tgtttgaaaa actggtgcag cgcgagccgg tgacgctggt gtcgctgatg
    27001 gaccactcgc cgggccagcg ccagttcgcc aatcgcgaga agtatcgcga atattatcag
    27061 ggcaaatact ccctcactga tgcgcagatg cagcagtacg aagaagagca actggcgctc
    27121 gccgcacgct ggtcgcagcc gaatcgcgaa tccatcgccg ccctgtgccg cgcgcgaaaa
    27181 attgcgcttg ccagccacga tgacgccacc cacgcccacg ttgctgaatc tcaccagctt
    27241 ggcagcgtga tcgccgaatt tcccaccacg ttcgaagcgg cggaagcctc gcgcaagcat
    27301 ggcatgaacg tgctgatggg cgcgccgaat attgtgcgcg gcggctcgca ctccggcaac
    27361 gtggcggcca gtgaactggc gcagcttggc ctgctggata tcctctcttc cgactactac
    27421 cccgccagcc tgctcgatgc ggcatttcgc gtcgccgatg acgagagcaa ccgctttacg
    27481 ctgccgcagg cggtgaagct ggtgactaaa aatccagcgc aggcgcttaa tctccaggat
    27541 cgcggggtga ttggcgaggg caaacgcgcg gacttggtgc tggcgcatcg caagggcaat
    27601 cacattcata tcgaccacgt ctggcgtcag ggtaaaaggg tgttctgatg atgggaaaac
    27661 tgatttggtt aatggggccg tccggctccg ggaaagatag cctgctggcg gaactccgcc
    27721 tgcgggaaca aactcagcta ctggtggcgc atcgctacat cacgcgcgac gccagcgccg
    27781 gaagcgaaaa ccatatcgct ctgagcgagc aggagttttt tacccgcgcg gggcagaacc
    27841 tactggcctt aagctggcac gctaacggtc tgtattatgg cgtcggcgtc gagatcgacc
    27901 tctggctgca cgccggattc gacgtgctgg tcaacggctc acgcgcccat ctgccgcagg
    27961 cgcgggcgcg ctatcaatcg gcgctgctgc ccgtctgttt acaggtttcg ccggagatcc
    28021 tccgccagcg cctggaaaac cgtggccgtg aaaacgccag tgaaattaac gcccgcctgg
    28081 cgcgcgccgc ccgctatact ccacaggatt gccatacgct caacaatgac ggcagcctgc
    28141 gccagtcggt cgacacgctg ctgacgctga ttcatcagaa ggagaaacac catgcctgct
    28201 tgtgagcttc gcccggccac gcagtatgat accgacgcgg tttacgcgct gatttgtgag
    28261 ctaaaacagg cggagtttga ccaccacgca tttcgcgtgg gctttagcgc caatctgcgc
    28321 gacccgaaca tgcgctatca tctggcgctg ctggatggcg aggttgtcgg catgatcggc
    28381 ctgcatttgc agtttcatct gcatcatgtc aactggatcg gcgaaattca ggaactggta
    28441 gtgatgccgc aggcgcgcgg cctgaacgtc ggtagcaagt tgctggcgtg ggcagaagaa
    28501 gaagcccgcc aggccggggc cgaaatgacc gagctttcga ccaacgtgaa gcgccacgac
    28561 gcgcaccgtt tctatctgcg agaaggctac gagcagagtc acttccgctt caccaaggcg
    28621 ctgtaacatg agtctgaccc tcacgctcac cggcaccggc gg
//
LOCUS       DAJVQG010000037        28560 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJVQG010000037 DAJVQG010000000
VERSION     DAJVQG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28560
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.037"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..928)
                     /locus_tag="OQM83_001481"
     CDS             complement(<1..928)
                     /locus_tag="OQM83_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCS1519775.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGG"
     gene            1419..1976
                     /gene="pinE"
                     /locus_tag="OQM83_001482"
                     /pseudo
     CDS             1419..1976
                     /gene="pinE"
                     /locus_tag="OQM83_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(2315..3163)
                     /locus_tag="OQM83_001483"
     CDS             complement(2315..3163)
                     /locus_tag="OQM83_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCS1519776.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3372..4007
                     /gene="yfhb"
                     /locus_tag="OQM83_001484"
     CDS             3372..4007
                     /gene="yfhb"
                     /locus_tag="OQM83_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCS1519777.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4032..4568
                     /gene="tadA"
                     /locus_tag="OQM83_001485"
     CDS             4032..4568
                     /gene="tadA"
                     /locus_tag="OQM83_001485"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCS1519778.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQPSTD"
     gene            complement(4565..6121)
                     /gene="mltF"
                     /locus_tag="OQM83_001486"
     CDS             complement(4565..6121)
                     /gene="mltF"
                     /locus_tag="OQM83_001486"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCS1519779.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            6379..10266
                     /gene="purL"
                     /locus_tag="OQM83_001487"
                     /gene_synonym="purI"
     CDS             6379..10266
                     /gene="purL"
                     /locus_tag="OQM83_001487"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCS1519780.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            10824..12251
                     /gene="qseE"
                     /locus_tag="OQM83_001488"
     CDS             10824..12251
                     /gene="qseE"
                     /locus_tag="OQM83_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCS1519781.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            12416..13129
                     /gene="qseG"
                     /locus_tag="OQM83_001489"
     CDS             12416..13129
                     /gene="qseG"
                     /locus_tag="OQM83_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCS1519782.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            13119..14453
                     /gene="glrR"
                     /locus_tag="OQM83_001490"
     CDS             13119..14453
                     /gene="glrR"
                     /locus_tag="OQM83_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCS1519783.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            14514..14852
                     /gene="glnB"
                     /locus_tag="OQM83_001491"
     CDS             14514..14852
                     /gene="glnB"
                     /locus_tag="OQM83_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCS1519784.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(14897..16087)
                     /gene="hmpA"
                     /locus_tag="OQM83_001492"
     CDS             complement(14897..16087)
                     /gene="hmpA"
                     /locus_tag="OQM83_001492"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCS1519785.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            16415..17668
                     /gene="glyA"
                     /locus_tag="OQM83_001493"
     CDS             16415..17668
                     /gene="glyA"
                     /locus_tag="OQM83_001493"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCS1519786.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            complement(17866..19059)
                     /locus_tag="OQM83_001494"
                     /pseudo
     CDS             complement(17866..19059)
                     /locus_tag="OQM83_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            19177..22454
                     /locus_tag="OQM83_001495"
                     /pseudo
     CDS             19177..22454
                     /locus_tag="OQM83_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            22551..23534
                     /locus_tag="OQM83_001496"
     CDS             22551..23534
                     /locus_tag="OQM83_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCS1519787.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            23557..25068
                     /locus_tag="OQM83_001497"
     CDS             23557..25068
                     /locus_tag="OQM83_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCS1519788.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            25093..26091
                     /locus_tag="OQM83_001498"
     CDS             25093..26091
                     /locus_tag="OQM83_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1519789.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            26124..27218
                     /locus_tag="OQM83_001499"
     CDS             26124..27218
                     /locus_tag="OQM83_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1519790.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            27230..28102
                     /locus_tag="OQM83_001500"
     CDS             27230..28102
                     /locus_tag="OQM83_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCS1519791.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(28150..>28560)
                     /locus_tag="OQM83_001501"
     CDS             complement(28150..>28560)
                     /locus_tag="OQM83_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCS1519792.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT         6784 a         7066 c         7758 g         6952 t
ORIGIN      
        1 gccccccttc agtgatgact ctgatatggc gcgaatgtaa attttgcggc aacatctggc
       61 gtaaaaacag tctgttttgg tctgacaggg aaatacgaaa ctgaggcccg ttaaaatttg
      121 gaaccgaagt ttgctgtatt aagaatagca gagtttgatc tgataagggg ttatttgcaa
      181 tctcaatagt tatatgagga tctaaacgaa aaagatttgt cgggattgta gttatttggt
      241 tgttttcaaa ataaaaaaat cttctcatga taatattttc ttggatgttt ggcaagttgc
      301 ttaatctatt gctatatgca aaaagaagtt gcagtctcct aggtaaaggg ggcagttcag
      361 taagatggtt gtgcgcaacc ttaagtatct taagattaga aggcagagat ggaagagttt
      421 gcagttcgtt gttcctaacc tcaagttttt ttaaattcaa gggtaatgaa ggcagtgtgg
      481 ataacctatt atactctgcg cttagtgatt tgatggtgtc tggtaataac ggcagattct
      541 ctaattgatt ttcggacaca tccaaagtta ttagataatg tggtaatgca gatatatgtg
      601 ttaagaaatt atctcttgca ataagttctt taatattttc aggtaaatca gtaaggatgg
      661 ttaatttatt acagctcaca tcaagctttt taatgcaagc agggagtaca ggtaacgagg
      721 aaagcgccaa ggctcgcagg tccagtttat gccccttaga ggtaagacat gcacgaagtc
      781 ttcttaccgc ctcagtacgc tgttcttccg gtaacccctg tttctcccaa tcatcccatt
      841 cggacaaata atggttgggg gatgtaattg tgctgatatg tggaaataaa caggatgaaa
      901 tatttctgag catattaatt tctcgcatat ggatatggaa ttagattgtg aataaattgt
      961 atttaatgta tctctcctaa tgaggagggt gattcagaat attcgccata agggaatgag
     1021 tgttatatca tgctttctac cctggtaagt atatttgctt ttttcgtata tttggttcat
     1081 ctttttttga tgttttacat attcacaatg cagaggattt ataccagaag acattagtct
     1141 tgagtaagat actacaacat ctccaactat tttagccagc ctgactactt gacttggtaa
     1201 tatttgttgc taataatcat gtataaaata agcttatata catagtaact tcagcaaagg
     1261 atttagtaat gatccaaatc atatctcaat gtactgtttt tttctttaaa aaaacagtac
     1321 attctacttt ataatgactg tgtattccca tggaaggaga tcacccaatt ctcccccccg
     1381 acttttgacg cagtgtcttg ttaacagggg gatttaccat gcttattggc tatgtacgcg
     1441 tatcaacaaa tgaccagaat accgatctac aacgtaatgc gttgaactgt gcaggatgcg
     1501 agctgatttt tgaagacaag ataagtggca caaagtccga aaggccgggg ctgaaaaaac
     1561 tgctcaggac attatcggca ggcgacacac tggttgtctg gaagctggat cggctggggc
     1621 gcagtatgcg gcatcttgtc gtgctggtgg aggagttgcg cgaacgaggc atcaactttc
     1681 gtagtctgac tgattcaatt gataccagca caccaatggg acgctttttc ttccatgtga
     1741 tgggtgccct ggctgaaatg gaacgtgaac tgattgttga acgaacaaaa gcaggactgg
     1801 aagctgctcg cgcataggga cgaattggcg gtcgccgtcc caaactttca ccagaataat
     1861 gggcgcaggc cgggcgacta attgcatcag gagtttctcg ccagaaggtg gcgatcatct
     1921 atgatgttgg cgaatcgact tttttgtata agaggtttcc agccagggtt atataaaacc
     1981 gcagacacgg cgtatacaag aacgtgctac ggttgactga aaagttaatg tggtatgtca
     2041 caggcctttt gcgccgtaaa gccccgtcct ttagggcgcg gaggacgtta aagaaagtgg
     2101 tgttctttgt gttgctgaac ttatgcaaaa tttttttgca ccataaatcc aacaacacat
     2161 tcaataacac attgctgtat cactatgaaa ataaatgtgt ttttatttga tttcacacat
     2221 atcacaattg atgcattttt tagtgattaa caacgccata aggaaactct caaaacaaca
     2281 caaaccgggc gggcattgta cgcgcattct ttactcagac cagttttttc accagcgctt
     2341 cactatgacg aatacgttct ggtgccagtt ccagatcctg ctgaatcagc gcaatgaaca
     2401 gcaaatccgt taacattccc tgagcgtgac aagcagagat tgaagcactg tttgtcgctt
     2461 gttcttcggc aatggtatac aggcaatgag aagcacgctg ttgcagggca ttcggagtaa
     2521 agccggtaat cgccagcact tttccgccca ctcgcagcat ctcatctgcc gccaggttta
     2581 actcgcgtcg tacaccggtg taggaaatgg ctaataacag atcgtcaggg gacgacgcct
     2641 gtactgttgc gagtagcgca tgcatatcgc gcactgcggc agcattgaag ccaatcttca
     2701 tcagcttcca ggcaaagttt tgcgccacca gacccgaagc gccaataccg gtcagaatta
     2761 tccgccgcgc agagcgcaac attgttacgc attcatgcag tttctcttca ctattaacgt
     2821 ttagcgttgc gtacatagcg gcagtatttt ctttaatcag tttttcgccg accagccgta
     2881 acggatcatc accgcggatt tggttatgaa tgggcacgga gggcgattcc ggctggcttg
     2941 ccagcgcttc actcaacgcc aacttaagcg ccggaaaacc tttatagccg agtttttgcg
     3001 cgaacttcac gacgctggac tgactgactc cggcttcgtt ggccagttgc tgagagctta
     3061 aatggcgcgc cgtatcaggt tgtagcagca gataatccgc cagtttttta tcactctggg
     3121 caagcccctg gtaacgctga cggatacgaa gtaagccgtt catatctttc ctcgcgatga
     3181 aatagtggcg acaacaggca atatgagaca cacgcgagtg aattttatat tccattgccg
     3241 ctaattgcca tgaattaaaa attccagtta gccatgatgc ggtcgattgt tgccggaaaa
     3301 gtgatgatct atctgttagg ctatgtagta gcgaaaatta atcaggatat ttcagtccag
     3361 aggagtatgg tttggcaact cacgagcgtc gtgtggtgtt ttttgactta gatggaacat
     3421 tgcatcagca ggatatgttc ggcagttttc tgcgctattt actgcgtcgc caaccgctga
     3481 atgcgttact tgtcctgccg ttgttaccga ttatagccat tgcgttattg ataaaaggtc
     3541 gtgcggcacg ctggccgatg agtctgcttc tgtgggggtg cacttttggt cacagcgaag
     3601 cacgtttaca gacgttgcag gccgatttcg tgcgctggtt tcgcgacaat gttaccgcct
     3661 ttccgctggt tcaggagcga ttaaccacct acctgttaag ttccgatgct gatatctggt
     3721 tgattaccgg ctctccgcag ccgctggttg aagcggttta tttcgatacg ccctggctgc
     3781 cgcgggttaa tcttatcgcc agccaaattc agcgtggcta tggtggttgg gtattgacga
     3841 tgcgttgtct gggacatgaa aaggtcgcac aactggagcg caaaatcggc actccgctgc
     3901 ggctgtacag tggctatagc gacagtaatc aggacaatcc gctgctttat ttctgtcagc
     3961 atcgttggcg agtaaccccg cgcggtgaac tccagcaact ggaatagagt aaagcatagc
     4021 gtccgtgtat aatgcgccgc gcttttataa ccggagtttt ctttttgtct gaagtcgaat
     4081 ttagccacga atactggatg cgtcacgcgc tgacgctggc gaaacgtgcc tgggatgagc
     4141 gggaagtgcc ggtcggcgcg gtattagtgc ataacaatcg ggtaatcggc gaaggctgga
     4201 accgcccgat tggtcgccat gatcccaccg cacatgcaga aatcatggcc ctgcggcagg
     4261 gtggtctggt gatgcaaaat tatcgtctga tcgacgccac gttgtatgtc acgcttgaac
     4321 catgtgtaat gtgtgccgga gcgatgatcc acagtcgcat tggtcgcgtg gtctttggtg
     4381 cgcgtgacgc gaaaactggc gctgcgggat ctttaatgga tgtgctgcat catccgggta
     4441 tgaatcaccg agtggaaatt acggaaggaa tactggcgga tgagtgcgcg gcgttgctca
     4501 gtgacttctt tcgcatgcgc cgccaggaaa ttaaagcgca gaaaaaagcg caaccctcga
     4561 cggattaatt ttgtttctct tcactccctt tcctggaaaa cagcagagag ggagaatggg
     4621 tcaaatagtt tgatgacgac tgggaaagaa acgagagaaa aggaaatttc tctttgccca
     4681 actccgtagg cgataccgcc ggataatcct gcgccagttg catcgccgct tctgtagcct
     4741 gcttctcttt ctcttgcaga taacccacca ggctaatctg atacttacga atattttcga
     4801 cataagcgta ggcttcatgt ccacgagcgt agccgtaagt cagcttgctg taatagggtt
     4861 tctggctaag taaaggcaga cgctgtttta cgtcagccca actgtcagga ttccctttgg
     4921 tttttgccgt cagggcgcgg gcatccagca tatgcgcata gcccatattg tacgcagcga
     4981 gggcaaacca gatccgctcg ttctccggca cactttccgg cactttactc atcatatcct
     5041 gcaaataacg cacgccaccg ctgatgctct gttcggcatc ggtacgatcc gtaatgccga
     5101 ggctttgcgc ggtatttttg gttaacatca tcatgccgcg cacacccgtc ggtgaagtgg
     5161 cctgtgcatc ccagtgcgat tcctgataag caatagcggc cagcaaacgc cagtcaattt
     5221 cttcggcgta tttctcaaac aggggcttta actgcggcag taccgcatcg acggcgcgta
     5281 aaaatgtgcg cgtatcgacg taatcaaaat catcgccatg ccccaggtat ttctcttcaa
     5341 tgcgtgccag cgtaccgtct tcattcattt cgttgaagaa gtcgagcagg gcggcggaaa
     5401 gggtattatc gccatctaac gggctaaacc aagtcaccgg ttgttcatcg gtgatatcga
     5461 gcgctacggc gagctccggg tgaacgcgct gaaacaggct gatggcgaca gaatcagcaa
     5521 tggtgtaatc gagttttcct tcgatgacat cttccattaa ttccgcagag ccttttttgt
     5581 cgtctacctt ccagcttaat tccgggaatt ttgtgtcttt cagggtttgg agatcgttaa
     5641 ccaccacgtg acccggtgca acggtgagtt gctccgccgt caggttgccc agtgtgcgcg
     5701 ggcgatactg actcacttta taaaccagtt gttgtgacac ggaataatag gtagggccag
     5761 gctgataatt ttttacccgc tcactgttat agaccagtcc tgccgccagc aggtcggcgt
     5821 taccattatc gaggtcgtca aacagctggc tgatattctg ccgcacggtc actttcagtt
     5881 ttacaccgag gtaatcggca aactgtttcg ccagttcgta atccaggcca aaaggtttcc
     5941 cgttgatttc gttataagtc aggggagtat gaatggtgct cacacgcaac tctccccgcg
     6001 cttgaatggc ggcgatacgg ttgtcggctt taccaaacca gggaatggat ggccagagag
     6061 cgaccgcgag cagcagtgcc agaatgccga tgaacagata attaatcttt aattttttca
     6121 attagttaat tctctgtgtc gtgcgcgtcc cagcttgaaa aaacgtaata atagtgaaag
     6181 gtttactcat aaatgagcgg cattttgcgt aaacctgcgc cagatggcaa cttattacag
     6241 ccattggcgg cacgcgttgc taattcacga tggtgatttt atttccacgc aaacggtttc
     6301 gtcagcgcat cagattcttt ataatgacgc ccgtttcccc cccttgggta caccgaaagc
     6361 ttagaagacg agagacttat gatggaaatt ctgcgtggtt cgcctgcact gtcggcattc
     6421 cgaatcaaca aactgctggc acgttttcag gctgccaggc tcccggttca caatatttac
     6481 gccgagtatg tccattttgc tgacctcaat gcgccgttaa acgatgatga gcacgcacaa
     6541 cttgaacgcc tgctgaaata tggcccggca ctcgccagcc acgccccgca aggcaaactc
     6601 ctgctggtga ccccgcgtcc tggcaccatc tctccctggt cttcgaaagc gaccgatatt
     6661 gcccataact gcgggctaca acaggtaaac cgccttgagc gcggcgttgc ttactatata
     6721 gaagccggta cgctgaccaa tgaacaatgg cagcaggtta ccgctgaact gcacgaccgc
     6781 atgatggaaa cggtcttttt tgctttagat gatgcagagc agttgtttgc ccaccatcaa
     6841 ccgactccgg ttaccagcgt tgatttgctg gggcagggcc gtcaggcgct gatcgacgct
     6901 aacctgcgtc ttggcctggc tctggcggaa gatgaaattg actatctgca ggatgctttc
     6961 acaaagcttg gtcgtaaccc gaacgacatc gagctgtata tgttcgctca ggcgaactct
     7021 gagcactgcc gccacaaaat ttttaatgcc gactggatta tcgacggtga acagcagccg
     7081 aaatcgctgt tcaagatgat caaaaatact ttcgaaacca cgccagatca cgttctctct
     7141 gcttataaag ataacgccgc cgtaatggaa ggttctgaag tgggccgcta ctttgctgac
     7201 cacgaaacgg gccgctacga tttccatcag gaaccggcgc atattctgat gaaagtcgaa
     7261 actcacaacc acccgacggc gatttctccg tggccgggcg cggcgacggg ttccggcggc
     7321 gaaattcgtg atgaaggcgc caccggacgc ggtgcgaagc cgaaggctgg tctggttggt
     7381 ttctccgttt ccaacctgcg aattcctggc ttcgaacagc cgtgggaaga agatttcggt
     7441 aagcctgagc gcattgtcac cgcgctggac atcatgaccg aaggcccgct gggcggcgcg
     7501 gcgtttaaca acgaatttgg tcgtccggca ctgaatggct acttccgtac ttatgaagaa
     7561 aaagtgaaca gccacaacgg cgaagagctg cgcggttatc acaaaccgat catgctggcg
     7621 ggcgggatcg gcaacattcg cgccgatcac gtacaaaaag gcgagatcaa cgtcggtgcg
     7681 aagctggtcg ttctcggcgg cccggcaatg aacatcggtc ttggcggcgg agcggcgtct
     7741 tccatggcgt caggtcagtc tgatgccgac ctcgactttg cttccgtaca gcgcgacaac
     7801 ccggagatgg agcgtcgctg ccaggaagtg atcgaccgtt gctggcagct tggtgatgcc
     7861 aacccaatcc tgtttatcca cgacgtcggc gctggcggtc tttctaacgc gatgccggaa
     7921 ctggtgagcg acggcgggcg cggcggtaaa tttgaactgc gcgatattct aagcgacgaa
     7981 ccgggcatga gcccgctgga aatctggtgt aacgaatccc aggaacgcta cgtgctggcg
     8041 gttgctgccg atcaattacc gctgtttgac gaactgtgta agcgtgagcg cgcaccctac
     8101 gcggtgattg gtgaagcgac cgaagaactg catctttctc tgcacgatcg tcattttgat
     8161 aatcagccga tcgatctgcc gctggacgtc ctgcttggta aaacgccgaa gatgacccgc
     8221 gatgtacaaa cgctgaaagc gaagggcgat gcgctggtcc gtgaagggat caccattgca
     8281 gacgcggtga aacgcgtact gcatctgccg accgtggcag agaaaacctt cctggtgacc
     8341 atcggcgacc gcagcgtaac cggcatggta gcgcgcgatc agatggtggg gccgtggcag
     8401 gtgccggtcg ctaactgcgc ggtcactacc gccagcctcg acagctacta cggtgaagcg
     8461 atggcgattg gcgagcgtgc gccggttgca ctgctggatt tcgccgcctc tgcccgtctg
     8521 gcggtcggtg aagcgttaac caatattgcc gcaacacaga ttggcgatat caaacgcatt
     8581 aaactttccg ccaactggat ggcggcggca ggccaccctg gtgaagatgc gggcctgtat
     8641 gaagccgtta aagccgtggg cgaagagctt tgtccggcgc tgggcctgac gatcccggtg
     8701 ggtaaagact ctatgtcgat gaaaacccgc tggcaggaag gtaacgaaga gcgcgaaatg
     8761 acgtcgccgc tgtcgctggt gatttctgca tttgcccgcg tggaagatgt gcgtcacacc
     8821 atcacgccac agctttctac cgaagataac acactgctgc tgattgattt gggcaaaggc
     8881 aataacgcgc tgggcgcaac ggcgctggcg caggtttatc gtcagcttgg cgacaaaccg
     8941 gcagatgtac gcgacgtcgc gcaactgaaa ggcttctatg acgcgattca ggcgctggtt
     9001 gcacagcgta agctgctcgc ataccacgac cgctctgatg gcggcctgct ggtaacgctg
     9061 gcggaaatgg cctttgctgg tcattgcggc attaacgcgg atattgcctc tctgggcgac
     9121 gatcgcctgg cggcgttgtt taacgaagaa ctgggtgcgg tgattcaggt tcgtgccgct
     9181 gaccgtgaag cggtcgagtc cgtactggca cagcatgggc ttgctgattg tgtccattat
     9241 gtagggcagg cggtttccgg tgaccgtttt gtgattaccg ccaacgggca gactgtattc
     9301 agcgaaagcc gcaccacgtt gcgtgtctgg tgggcagaaa ctacctggca gatgcagcgc
     9361 ctgcgtgaca acccggagtg tgctgaccag gagcaccagg cgaaatcaaa cgacgccgat
     9421 ccgggcctga atgtgaaact gtcgttcgat atcaacgaag atgtggcagc accgtatatt
     9481 gccactggcg cacgtccgaa agttgctgtg ctgcgtgagc agggcgtgaa ctcgcatgtt
     9541 gaaatggcgg cagctttcca ccgtgcaggc tttgatgcta tcgacgtgca tatgagtgac
     9601 ctgctgaccg gacgcacggg cctggaagat ttccacgccc tggtcgcgtg cggtggtttc
     9661 tcctacggtg atgtgctggg tgccggtgaa ggttgggcga agtcaatcct gttcaatgac
     9721 cgtgtacgcg atgagtttgc aaccttcttc caccgtccgc aaacgctggc gctgggggta
     9781 tgtaacggtt gccagatgat gtctaacctg cgtgaactga tcccggggag cgagttgtgg
     9841 ccacgttttg tgcgtaacac ctccgatcgc tttgaagcgc gtttcagcct ggttgaagta
     9901 acccaaagcc cgtctctgct gttgcagggg atggtgggct cgcaaatgcc gattgctgtc
     9961 tctcatggtg aagggcgcgt ggaagtgcgt gatgcggcgc atctggcggc actggaaagc
    10021 aaagggctgg tggcactgcg ctatgtcgat aacttcggca aagtcactga aacctacccg
    10081 gctaacccga acggttcacc gaacggtatt acggcggtta cgactgaaag tggtcgagtc
    10141 accattatga tgccgcaccc ggaacgtgtt ttccgtactg tcagcaactc ctggcatccg
    10201 gaaaactggg gcgaggatgg cccatggatg cgcattttcc gcaatgcgcg taagcagttg
    10261 gggtaagtca tcagttcatt ggttttgcag cccggatgcg gcattaagca ccaaccacta
    10321 tcataagctc ccaactccgg gagctttttt gtgtctgtaa atcacgacaa tgggtggttt
    10381 accgtgtcgc tttctggcga cacttaaatg attgatttta ttgcaatcta ataagtttat
    10441 cttaagttgt ctctttttag cgacacagtg gctgatttac attcaaatag cgaagaagcc
    10501 ataaatccct caaagcaacg tataatcagt acgttaccaa actattttct ttattggcac
    10561 agttactgca taatagtaag cagtggctca ttcaccgact tatgtcagcc ccttcgggac
    10621 gtgctacata aaattcgaat gacgcacaac aaggtgcctg ccgtccaact tctgatatca
    10681 gcgtagctat atcaaccatc gggcgaaacg tcgagttagg caccgcctta ttccataaca
    10741 aagccgggta attcccggct ttgttgtatc tgaacttccc ctcggttagc atcaggctat
    10801 tcgcgtctga cgagagtaac accttgaaac gctggcccgt ttttccccgc tcattacgac
    10861 aactggtaat gctggcattt ttgctaattc tactacccct gttggtgctg gcatggcaag
    10921 cctggcaaag cctgaatgcg cttagcgatc aggcggcgct ggttaaccgc actacgctta
    10981 ttgatgcccg gcgtagtgaa gcgatgacca acgcggcgct ggagatggag cgtagctacc
    11041 gtcagtattg cgtgctggac gaccctacgc ttgcgaaggt ttatcaaagc cagcgcaagc
    11101 gttacagcga aatgctcgat gcccacgcag gcgtactgcc agacgataaa ctttaccagg
    11161 cattgcgtca ggatttgaac aatctggccc aacttcagtg taacaacagt ggtcccgatg
    11221 ctgccgccgc cgcgcgtctt gaagcctttg ccagtgccaa taccgaaatg gtgcaggcca
    11281 cgcgcacagt ggtgttctct cgtgggcagc aacttcagcg tgaaatcgcc gaacgtgggc
    11341 aatattttgg ttggcaatcg ctggtgctat ttctggtgag tctggtgatg gtgctacttt
    11401 tcacgcggat gattatcggg ccggtgaaaa atatcgagcg catgatcaac cgtctggggg
    11461 aagggcgttc tctgggcaat agcgtttcgt tcagtggacc gagcgagtta cgctcggttg
    11521 ggcaacgtat tctttggtta agtgagcgcc tgtcatggct ggaatcccaa cgccatcaat
    11581 ttttaagaca tttatctcat gaattaaaaa cgccactggc gagtatgcgc gagggcactg
    11641 aattactggc tgaccaggtt gtcgggccgc ttacgccaga gcaaaaagag gtggtgagca
    11701 ttcttgatag cagcagccgc aatttgcaaa aactgatcga acaactgctt gattacaacc
    11761 gtaaacaggc ggacagtgcg gtggaactgg agaatgttga attagcaccg ctggtggaga
    11821 cagtggtttc tgctcatagc ctgcccgcac gggctaaaat gatgcatacc gacgtcgatc
    11881 tcaaagcaac agcttgcctg gcggagccaa tgctgctgat gagcgtactg gataatcttt
    11941 actccaatgc ggtgcactac ggggctgaat ccggtaacat ttgccttcgc agcagttcac
    12001 atggtgcgcg ggtttatatt gatgtcatca atacaggcac gcccattccg caagaggaac
    12061 gcgccatgat cttcgaaccc ttttttcagg gaagccacca gcgaaaaggg gcggtgaagg
    12121 gcagcggtct gggattaagc attgccaggg attgtattcg ccgtatgcaa ggggaactgt
    12181 atctggtcga cgagagcggg caagacgttt gtttccgcat tgaattaccg tcgtcgaaaa
    12241 acacgaaata atgctaacag cctatcctat caggctattt tatttgtcag ttcggccccg
    12301 ggcagtgctc gaaatcttca cgtacgatat gtacgctccg gtttctccgc gctgtccatg
    12361 tccagactgt ctgcaacaat tacgcccact gggataggct ttaagtctgg tgaatatgcg
    12421 acacattttt caacgattat tgccccgacg gctgtggctg gcagggctgc catgtctggc
    12481 attgctgggt tgcgtgcaga atcacaataa gccagccatt gatacgccag cagaagaaaa
    12541 aattccggtt tatcaactgg ctgactatct ttctactgaa tgtagcgaca tctgggcgct
    12601 gcaaggtaaa tcaaccgaaa ccaatccgct ttactggctg cgggcgatgg attgtgctga
    12661 tcgtttaatg cctgcgcagt cgcgtcagca agctcgccaa tatgacgacg gcagctggca
    12721 aaataccttt aaacaaggga ttttgctcgc cgacgccaaa attacgccgt atgagcgccg
    12781 ccagctggtc gcgcgtattg aagcgttaag taccgagatc ccggcacagg ttcgcccact
    12841 gtatcaactc tggcgcgacg gtcaggcgct gcaactgcaa ctggcggaag agcgccaacg
    12901 ttacagtaaa ctccagcagt ccagcgacag cgagctggat acattgcgcc agcaacatca
    12961 cgttctacag cagcaactgg aactcaccac ccgcaagctg gaaaacctga ccgatattga
    13021 acgccagctc tcgacccgca aaccgactgg aaatttctcg ccggatacgc cgcacgagag
    13081 tgaaaagccc gcgccatcca cccatgaggt cactcctgat gagccataaa cctgcgcatt
    13141 tattattggt cgatgacgat ccgggattgc tgaaactgct tggcctgcgc ctgaccagcg
    13201 aaggctacag tgtggtcacg gcggaaagtg gcgctgaagg attacgggta ctgaatcgcg
    13261 aaaaagtaga tttagttatc agcgacctgc ggatggatga aatggacggt atgcagctat
    13321 ttgctgaaat ccagaaagtg cagccgggaa tgccagtaat tattcttact gcgcatggtt
    13381 ctatccccga tgccgttgct gcaacacagc agggcgtttt tagtttcctc accaagcctg
    13441 tcgataaaga tgcgctatat caggcaattg acgatgcgct ggagcaatcc gcgccagcca
    13501 ccgatgaacg ctggcgcgag gcaattgtca cccgcagccc gctgatgctg cgtttgctgg
    13561 aacaggcgcg gctggtggcg caatcagacg tcagcgtttt gattaacggt cagagcggca
    13621 ccgggaaaga gattttcgcc caggctatcc acaacgccag cccgcgcaac agcaaaccat
    13681 ttattgctat taactgtggc gcattgcccg agcaattgct ggagtcggag ctgtttggtc
    13741 atgcgcgtgg cgcgtttact ggcgctgtca gcaatcgcga aggtttattc caggcggcgg
    13801 aaggcggaac gctatttctc gatgaaattg gcgatatgcc tgcgccgtta caggtcaaac
    13861 tgctgcgcgt gttgcaggag cgtaaagtgc gcccgctggg cagtaaccgc gatattgata
    13921 tcaatgtgcg gattatttct gccactcacc gtgatctgcc aaaagcgatg gcgcgcgggg
    13981 aattccgcga agatctctat taccgcctca acgttgtcag cctgaaaatt ccggcactgg
    14041 cggagcgcac agaagacatt ccgctactgg caaatcacct gttgcgccag gcggcagagc
    14101 gacataaacc gtttgtccgc gcgttctcta ccgatgcgat gaaacgcctg atgaccgcga
    14161 gctggccggg taatgtgcgc cagttggtca acgtgattga acagtgcgtg gcgctgacct
    14221 catctccggt gattagtgat gcgctggtgg agcaggcgct ggagggtgaa aatacggcgc
    14281 tgccaacctt tgttgaggca cgtaatcagt ttgaactcaa ctatttgcgt aagctgctgc
    14341 aaatcaccaa aggcaacgtc acccacgcgg cgagaatggc ggggcgcaac cggacagaat
    14401 tttataaact gctttcccga cacgagctgg atgcaaacga tttcaaggaa tgaattggcg
    14461 ttatgtgtta cgtttagcag atcaaaagac aggcgacctt ttcaaggaat agcatgaaaa
    14521 agattgatgc gattataaaa cccttcaagc tggacgatgt ccgcgaagca ctggccgaag
    14581 tcggtattac cggcatgacg gtgaccgaag tgaaaggctt tggtcgccag aaaggccata
    14641 ccgagctgta ccgcggcgcg gagtatatgg tggattttct gccgaaagtg aaaattgaga
    14701 ttgtcgtacc ggacgacatt gtcgatacct gtgtcgatac cattattcgc acggcgcaaa
    14761 ccggcaaaat cggtgacggt aaaatcttcg tctttgacgt ggcacgggtc attcgcatcc
    14821 gtaccggtga agaggacgac gcggcaattt aaaccgcgtc aagggttgcc ggatgtttcc
    14881 atccggcaac atcaaattac agcaccttat gcgggccaaa gcattcgtaa tgaatgtttt
    14941 cctgcttcac gcccagatcc actaactgtt tcgcggtaaa ctgcatgaag ccaaccgggc
    15001 cgcatagata gaactgcatt gtcggatcgc tgaacgcacc ttccagtttg ctcaaatcca
    15061 tcagaccttc gctatcaaac tgacctttag cgcgatcggc ttcgctcggc tgacgatgcc
    15121 aggtgtgcgc ggtaaagcgc ggcagtgact gccccagttc cttaacttca tcggcaaagg
    15181 cgtgaacatc gccattttct gccgcatgga accagttcac ttgtgctgtg tggcctgctt
    15241 ttgccagcgt gtcgagcatt gccagcattg gcgtttgacc aacaccggca gagattaacg
    15301 tcactggtgt gtcatctgcg acagccataa agaaatcacc tgccggagcg accagtttca
    15361 cgacatcgcc aacattggcg tgattgtgca accagttgga tacctgccca ccctcttcgc
    15421 gtttcaccgc aatacgatag cctttgccat ccggtttgcg agtcaaagag tactgacgaa
    15481 tttcctgatg cgggaaacct tccggcttca gccagacgcc gagatattgc cccggacggt
    15541 attctgccac tgcgccaccg tcgaccggct ccagttcgaa gctggtgata agcgcgctgc
    15601 gcggtgtttt agccacaatg cggaaatcgc gagtaccttc ccaaccaccg gctttgctgg
    15661 cgttttcgtt atagatttcc gcctcgcgat tgataaatac attagccagt acaccatagg
    15721 ctttacccca cgcgtccagc acttcctgcc ccgggctgaa catttcgtcc agcgttgcca
    15781 acaggtgttc accgacgatg ttgtactgtt ccggtttgat ctggaagctg gtgtgcttct
    15841 gcgcgatttt ttctaccgct ggcagcagcg caggcaggtt ttcaatatta ctggcgtagg
    15901 cggcaatagc gttaaacagg gcttcacgtt gatcgccatt acgctggtta ctcatgttaa
    15961 aaatttcttt gagttctggg ttatgagtaa acatacggtc gtagaaatgg gcggttaact
    16021 ttggccccgt ttccaccagt aaagggatgg tggcttttac tgtagcgatg gtttgagcgt
    16081 caagcatatg gtcttccttt ttttgcatct taattgatgt atctcaaatg catcttataa
    16141 aaaatagccc tgcaatgtaa atggttcttt ggtgtttttc agaaagaatg tgatgaagtg
    16201 aaaaatttgc atcacaaacc tgaaaagaaa tccgtttccg gttgcaagct ctttattctc
    16261 caaagccttg cgtagcctga aggtaatcgt ttgcgtaaat tcctttgtca agacctgtta
    16321 tcgcacaatg attcggttat actgttcgcc gttgtccaac aggaccgcct ataaaggcca
    16381 aaaattttat tgttagctga gtcaggagat gcggatgtta aagcgtgaaa tgaacattgc
    16441 cgattatgat gccgaactgt ggcaggctat ggagcaggaa aaagtacgtc aggaagagca
    16501 catcgaactg atcgcctccg aaaactacac cagcccgcgc gtaatgcagg cgcagggttc
    16561 tcagctgacc aacaaatatg ctgaaggtta tccgggcaaa cgctactacg gcggttgcga
    16621 gtatgttgat atcgttgaac aactggcgat cgatcgtgcg aaagaactgt tcggcgctga
    16681 ctacgctaac gtccagccgc actccggctc ccaggctaac tttgcggtct acaccgcgct
    16741 gctggaacca ggtgataccg ttctgggtat gaacctggcg catggcggtc acctgactca
    16801 cggttctccg gttaacttct ccggtaaact gtacaacatc gttccttacg gtatcgatgc
    16861 taccggtcat atcgactacg ccgatctgga aaaacaagcc aaagaacaca agccgaaaat
    16921 gattatcggt ggtttctctg catattccgg cgtggttgac tgggcgaaaa tgcgtgaaat
    16981 cgctgacagc atcggtgctt acctgttcgt tgatatggcg cacgttgcgg gcctggttgc
    17041 tgctggcgtc tacccgaacc cggttcctca tgctcacgtt gttactacca ccactcacaa
    17101 aaccctggcg ggtccgcgcg gcggcctgat cctggcgaaa ggtggtagcg aagagctgta
    17161 caaaaaactg aactctgccg ttttccctgg tggtcagggc ggtccgttga tgcacgtaat
    17221 cgccggtaaa gcggttgctc tgaaagaagc gatggagcct gagttcaaaa cttaccagca
    17281 gcaggtcgcg aaaaacgcta aagcgatggt agaagtgttc ctcgagcgcg gctacaaagt
    17341 ggtttccggc ggcactgata accacctgtt cctggttgat ctggttgata aaaacctgac
    17401 cggtaaagaa gcagacgccg ctctgggccg tgctaacatc accgtcaaca aaaacagcgt
    17461 accgaacgat ccgaagagcc cgtttgtgac ctccggtatt cgcgtgggta ctccggcgat
    17521 tacgcgtcgc ggcttcaaag aagcagaagc gaaagaactg gctggctgga tgtgtgacgt
    17581 gctggacagc atcaatgatg aagccgttat cgagcgcatc aaaggtaaag ttctcgacat
    17641 ctgcgcacgt ttcccggttt acgcataagc gaaacggtga tttgctgtca atgtgctcgt
    17701 tgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaaactttg caaattcaat
    17761 atattgcaat atccgtgtag gcctgataag cgtagcgcat caggcaatct taggtatgtg
    17821 atcatcaagg cttccttcgg gaagcctttc tacgttattg cgccatcaaa tctgtcgtaa
    17881 ctgcgcctca acatacaaat agccaatccc caacacctgt tgtgcgcggc ttaattgccc
    17941 aaagccaatt tgcgttgctt tcacgctcgg cccttcgtcg cggtcgaacg ggttaaatcc
    18001 cgtctggcga ataatggtat taagccagtg ctcaccaaag gcacaactgc gaccgtacaa
    18061 ccaaatctga ttaatattga ggatgttgag gaagttatac agactcaaac caatggcatt
    18121 ggcactgcga tcaacccaac tggtgatcca cggttctcca ctctaccagg cagcgattaa
    18181 ctgcgctgta gtcagttttt ccggatcaag ctgagtatta accggttgtg attttagcca
    18241 tacccgcgcc tgttttttta atgcacttaa cgaggcgacg gtttccaggc agccgtaacg
    18301 tccacagtcg cagacgacgc catccggatt aacgatggtg tgactaatct gtccgctacc
    18361 atacaaactg ccgcgataaa tttgctcgtt aatcacgaac gacgagccaa tgccgtaatc
    18421 aacgttgatc acgcagaaat cccgtttctg cgaattattt tgccatttct ccgccagcgc
    18481 cagcatcacg cagtcattat cgaccatcac ccgaatgccg agcttctctt ccagcagata
    18541 cttcacctca accggcgttg tccacggcgc ttgcggcatg gtttgcgaca cgccggtcac
    18601 cggatcaacc tgaccgtgga ttgccagcgc cagattgatg gtgcggtccg gccacaattt
    18661 acggtggcga tgccagcatt tttcgatttc ggacagcagc gcctgcggcg tcggtgcatc
    18721 aatctgtaaa tactcaaatt cacctttcgg acttaaacaa gcattagcaa cctgacactc
    18781 aatactggtg ggcgtcacgt tcaggcacag cgtccagtca ccttccggcg caatcagcca
    18841 tgtaccgcta ctatgcccgc gcgtctggct ttcatcgtcg atattcacca cccgtttttc
    18901 gctttccagt tcctgcaaaa tattactgac tgccggaatc gaaatttgcg ccagccgggc
    18961 cagcgttgat ttattggcgc gcttttgccg gtacagcagt tccagaatca cgcatttgtt
    19021 atggtggcga atctgttgat tgttaatgca ggctctcatc ttcactaaac cttgtttagt
    19081 taattgcgtg attattccgc gaaaacattg ccagattgtt caatacactg ccacaaatct
    19141 tttaattcag tatgtcttgt taatattgag ggcaccatga ctccagtaaa agtgtggcaa
    19201 gagcgcgttg agatcccgac ctatgaaacc gggccgcagg atatacatcc catgttcctg
    19261 gaaaatcgcg tttatcaggg atcgtccggc gcggtttatc cctacggcgt gaccgatacg
    19321 ctgagcgagc agaaaaccct gaaatcctgg caggcggtgt ggctggaaaa cgactacatc
    19381 aaagtgatga tcctgccgga actgggcggt cgggtgcatc gcgcgtggga taaagtgaaa
    19441 cagcgcgatt ttgtttatca caatgaagtc attaaacctg cgctggtggg gctgctgggg
    19501 ccgtggattt ccggtgggat tgagtttaac tggccgcaac atcatcgccc gaccaccttt
    19561 atgcccgttg atttcaccct cgaagcccat gaagacggtg cacagacggt gtgggtaggc
    19621 gaaacggagc cgatgcatgg tttacaggtg atgacaggtt tcaccctgcg ccctgggcgg
    19681 cgctggaaat cgccagccgc gtctataacg gcaacgccac gccgcgtcat ttcttgtggt
    19741 gggccaaccc ggcagtgaaa gggggtgaag ggcatcagag cgttttccca ccggatttga
    19801 ctgcggtgtt tgatcacggc aaacgggccg tctccgcttt ccccatcgct accggcactt
    19861 actacaaagt ggactactcc gctggagtgg acatttctcg ctataaaaat gtgcccgttc
    19921 caacctcata tatggccgaa aaatcgcagt acgattttgt cggcgcgtgg tgtcacgatg
    19981 aagatggcgg tttgctgcac gttgccaacc accatattgc gccaggtaaa aaacagtgga
    20041 gttggggaca cagtgaattt ggccaggcgt gggataagag tctgaccgac aataacggcc
    20101 cgtatatcga actgatgacc ggtatttttg ccgataacca gcccgatttt acctggcttg
    20161 atgcttacga agagaagcgt tttgagcagt atttcctgcc ttatcattct ctgggcatgg
    20221 tgcaaaatgc ctcccgcgat gcggtgataa aactccagcg tagtgagcag gggattgagt
    20281 gggggctgta tgccatctct ccgttgaacg gataccgcct ggcgatccac gaaatcggca
    20341 aatgcaacgc gttacttgat gatgccgtgg cactgatgcc tgcgaccgcc atccagggcg
    20401 tgttgcacgg tatcaatcct gataggctga ccattgagct ttccgatgcc gacggcaata
    20461 ttgtactgag ttatcaggaa catcagccgc aagcgttgcc gttaccggac gtcgccaaag
    20521 cgccactggc agcacaagac attaccagta cagatgaagc ctggtttatc ggtcagcatc
    20581 tggagcaata tcatcacgcc agccgttcac cgttcgatta ctacctgcgc ggcgtggcgc
    20641 tggacccgct ggattatcgc tgtaacctgg cgctggcgat gctggaatat aaccgcgcag
    20701 atttcccgca agcggtggcg tatgccagtc aggcgctgaa acgcgcacat gcgctgaaca
    20761 aaaacccgca gtgtggacaa gcaagtttga ttcgcgccag tgcttacgaa cgtcagggac
    20821 aatatcaaca agccgaagag gatttctggc gtgcggtctg gagcggcaac agcaaagccg
    20881 gtggctatta tggcctggca cgactggcgg cgcgtaatgg taacttcgac gctggtctgg
    20941 atttttgcca acaaagtctt cgcgcctgcc caaccaatca ggaagtgctt tgcctgcata
    21001 atctgctgct ggtgttaagt ggtcgtcagg acaacgcgcg tttgcagcgc gcgaaactgc
    21061 tgcgtgatta tccgctgaac gccattctgt ggtggctgaa ctggttcgat ggtcgtagcg
    21121 aatcagccct cgcgcagtgg cgcggtctgt gtcagggacg cgacgttaac gccctgatga
    21181 ccgccgggca actgattaac tggggaatgc ccactctggc gacagagatg ctgaacgcac
    21241 tggactgtca gcgcacgtta tcgctttatc tacaagccag cttgctgccg aaagccgaac
    21301 gcggcgaact ggtcgcaaaa gccattgatg tcttcccgca gtttgtccgt ttcccgaata
    21361 cgctggaaga agtggcggcg ctggagagta ttgaagagtg ctggtttgcc cgtcatttac
    21421 tggcctgttt ctactacaac aagcgcagct acaacaaagc cattacttta tggcaacgtt
    21481 gcgtagagat gtcgccggag tttgccgatg gctggcgcgg gttggcgatc catgcgtgga
    21541 ataagcaaca cgattatgaa ctggccgcgc gttatcttga taatgcttat cagcttgcgc
    21601 cgcaggatgc acgtctgctt ttcgaacggg atttgctgga taagttaagt ggagccacac
    21661 cggagaaacg actggcgcgt ctggaaaata atctggaaat tgcgctgaaa cgcgacgaca
    21721 tgaccgcaga actgctcaat ttgtggcatc tcacgggtca ggcagacaaa gcggcggaca
    21781 ttctcgccac gcgcaaattc cacccgtggg aaggcgggga agggaaggtc accagtcagt
    21841 ttatcctcaa ccagttatta cgcgcctggc agcatcttga tgccagacag ccgcagcagg
    21901 cctgcgaact gcttcatgcc gcgctgcatt atccggagaa tttaagcgaa ggccgtttac
    21961 cggggcaaac tgataacgac atctggttct ggcaggcgat atgcgccaac gcgcagggcg
    22021 atgaaactga agcgacgcgt tgtttacgtc tggcggcgac cggcgatcgc accattaaca
    22081 tccacagtta ttacaacgat cagccggttg attatctctt ctggcaagga atggcgctgc
    22141 gactgctggg cgaacaacaa atcgcacagc aactgtttag tgaaatgaaa cagtgggcgc
    22201 aagagatggc gaaaaccagt attgaggcgg atttctttgc tgtttcacaa cctgacctgt
    22261 tgtcgctgta tggcgattta caacagcagc ataaagaaaa atgcctgatg gtggcgatgc
    22321 tggcgtccgc gggactcggg gaggttgcgc aatatgaatc tgctcgcgct gaattgacgg
    22381 cgattaatcc ggcctggccg aaagcggcat tattcaccac cgtgatgcct tttattttta
    22441 actacgttca ctaaacccta ttaactgtaa tatttgagcg gcacgcgctg ccgctcatca
    22501 caaaataatt agaataagcc gttgttaata aaaactctac aggaggcact atgcctaaaa
    22561 aaatgagaac aacccgtaat ctattattaa tggctacgct attaggtagc gcactatttg
    22621 ccagggctgc ggataaagaa atgaccattg gtgcaatata ccttgatacc ctgggatatt
    22681 acgctggagt gcgtcagggc gttcaggatg cggcaaaaga ttcttcagta caggtacagt
    22741 taattgaaac taacgcccag ggtgatattt cgaaagaaag tacctttgtt gataccctcg
    22801 tggcgcgtaa tgtcgatgcc attattttat cggcagtgtc tgaaaatggc agtagccgta
    22861 ccgttcgtcg cgccagtgaa gcgggtattc cggtgatttg ctacaacacc tgtattaatc
    22921 aaaagggtgt cgataaatat gtctcggcgt atctggtcgg cgatccactg gaatttggta
    22981 aaaaactggg taacgctgcc gccgattatt ttattgccaa taaaattgac cagccgaaaa
    23041 ttgccgtcat caattgcgaa gcctttgaag tttgtgtgca gcgacgtaaa ggatttgaag
    23101 aagtattaaa atcccgcgtt cccggcgcgc aaattgtcgc taatcaggaa gggactgttt
    23161 taaataaagc gatttccgtt ggtgaaaaac tgattatctc cacgccggat ctcaacgcca
    23221 ttatggggga gtcaggcggt gcgacactcg gcgcggtaaa agcggtacgt aatcaaaatc
    23281 aggccggaaa aattgctgtt ttcggttcgg atatgacaac cgaaattgct caggagctgg
    23341 aaaacaatca ggtgctgaaa gcggtagtgg atatttccgg taagaaaatg ggcaatgctg
    23401 ttttcgcgca aacattgaag gttatcaata aacaagccga cggtgaaaaa gtgattcagg
    23461 tgccaatcga tctctatacc aaaacggaag atggtaaaca gtggctggca acgcacgttg
    23521 atggtctgcc ctaatcgctg attcgcagag gtccctatgt tcacggcaac agaggcagtc
    23581 ccggtagcaa aagtggtggc aggaaataag cgttaccccg gcgtcgttgc gctggataac
    23641 gttaacttca cgctcaataa aggcgaagtt cgtgcgctgt taggtaaaaa cggcgcgggc
    23701 aaatcgactc tcattcgaat gcttaccggc agcgaacgcc cggatagcgg tgatatctgg
    23761 attggcgaga cgcgactgga aggtgacgaa gctacgttga ctcgccgtgc cgctgaactg
    23821 ggggttcgtg cggtttatca ggaattaagt ctggtggaag ggctgacggt ggcagaaaac
    23881 ctctgccttg gtcaatggcc tcgccgcaac ggcatgattg attacctgca aatggcgcag
    23941 gatgcccaac gttgcttaca ggcgctgggc gttgacgtta gtcctgaaca acttgtttca
    24001 acgctaagcc cggcgcaaaa gcaactggtg gaaattgcac gggtgatgaa gggcgagccg
    24061 cgcgtggtca ttcttgatga acccaccagc tcgcttgcca gtgcagaagt tgaactggtg
    24121 atcagtgcgg tgaaaaagat gtcggcactg ggcgtggcgg tgatttatgt cagccaccgg
    24181 atggaagaaa tccgccgcat tgcctcctgt gccaccgtta tgcgcgatgg tcaggtggcg
    24241 ggcgatgtga tgctcgaaaa cacctccacg catcatattg tgtcgctaat gctagggcgt
    24301 gatcacgttg atattgcgcc ggttgcccct caggaaattg tggatcaggc cgtactggaa
    24361 gtccgtgcgt tacgccataa gcccaagctg gaggatatca gctttacgtt acgtcgcggc
    24421 gaagtgcttg gcattgctgg cctgctgggg gcagggcgca gtgaattgct gaaggcgatt
    24481 gttgggctgg agacgtatga acagggcgaa attgttatca acggcgagaa aatcacgcgc
    24541 cccgattacg gcgacatgct gaaacgcggc attggctata cgccagaaaa ccgcaaagaa
    24601 gcggggatca ttccctggtt gggcgttgac gaaaatacag tgctgaccaa tcggcaaaaa
    24661 atcagcgcca acggagtgct gcaatggtcc accatccgcc gcctgaccga agaggtgatg
    24721 cagcggatga cggtcaaggc cgctagtagc gaaacgccca tcggcacgct ttctggtggc
    24781 aatcagcaaa aagtggtgat cggtcgttgg gtctatgccg ccagccagat tttgttgctc
    24841 gacgagccaa cgcgcggcgt cgatatcgaa gccaaacagc agatttaccg tattgtccgt
    24901 gagctggctg ccgaaggaaa aagcgtggtg tttatctcca gtgaagtgga ggagttgccg
    24961 ctggtgtgtg accgcatcct gttattacag cacggcacgt tctcgcagga gtttcactcg
    25021 ccggtcaatg tggatgagct gatgtccgcc attctgtctg tgcactgatg aattttaccg
    25081 aggaacctga agatgtctgc ttcgtcatta ccattgccgc agggcaagag cgtctcgctc
    25141 aaacaatttg tcagtcgcca tattaatgag atcggtttgc tggtggtgat tgccattctt
    25201 tatctggtct tctccctgaa cgcgcctggc tttatctcat tgaataacca gatgaacgtg
    25261 ctgcgcgatg ccgccaccat tgggattgcc gcctgggcga tgacgctgat tattatctcc
    25321 ggtgaaattg atgtcagcgt tgggccgatg gtggcttttg tctcggtgtg cctggcattt
    25381 ttgctgcaat ttgaagttcc gttggcgatt gcttgtctgc tggtgttgct gttaggcgcg
    25441 ctgatgggaa cgctcgccgg ggtgctgcgc ggcgtgttta acgtaccaag tttcgttgcc
    25501 acactggggc tgtggagcgc cctgcgcgga atggggctgt ttatgaccaa cgcactgcca
    25561 gtgccgattg acgaaaacga ggtgctggac tggctgggcg gacaatttct cggtgtgcca
    25621 gtatccgcgc tgatcatgat ggtgttgttt gcgctgtttg tgttcattag ccgaaaaacc
    25681 gccttcgggc gctcggtttt tgccgttggc ggtaatgcca cggcggcgca gttgtgcggt
    25741 attaacgttc gtcgggtgcg cattcttatc tttacccttt cgggattatt agcggcggtg
    25801 accggcattt tgctggcggc acgcctcggt tccggtaacg caggtgccgc aaacggtctg
    25861 gagtttgacg tcatcgccgc ggtcgtggtc ggcggtacgg cactttccgg cggtcgcggt
    25921 tccctgttcg gtacattgct tggtgtgctg gtgattacgc taatcggtaa cggtctggtg
    25981 ctgctcggta ttaactcctt tttccagcag gtggtacgcg gcgtcatcat cgtggtggcg
    26041 gtgctggcga atatcttgct gacccagcga agcagtaaag cgaaacgcta aaccttaacg
    26101 cccctggcgc gggtgcggca gggatgaaaa ttaatcgtat tgttaatgag ggatttatga
    26161 aaacgatgct ggcagcttat ttaccaggaa attcaaccgt cgatctgcgg gaagttgcgg
    26221 tgccgacgcc ggggattaac caggtactga tcaaaatgaa atcctccggg atttgcggaa
    26281 gcgatgtcca ctatatctat catcaacacc gtgccacggc ggcggcaccc gataaaccgt
    26341 tataccaggg atttatcaac ggtcatgaac cgtgcgggca gattgtggcg atggggcaag
    26401 gctgccgcca ttttaaagag ggcgaccgcg tgctggtgta tcacatttct ggctgtggtt
    26461 tttgcccgaa ctgccgccgc ggctttccta tctcttgtac tggcaaagga aaagcggctt
    26521 acggctggca gcgtgacggc ggtcatgccg aatatctgct ggcggaagaa aaagatctga
    26581 tcctcctgcc ggatgcgctg agctacgaag atggtgcgtt tatcagttgc ggcgttggca
    26641 cggcctatga agggattttg cgcggcgaag tttccggcag cgataacgtg ctggtggtcg
    26701 gtctggggcc ggtcggcatg atggcgatga tgctggcgaa aggtcgcggt gcaaaacgga
    26761 tcatcggcgt tgatatgctg ccggaacgtc tggcgatggc aaaacagtta ggggtgatgg
    26821 atcacggcta tttagcaacc accgaaggtc tgccgcagat tatcgccgaa ctcacccacg
    26881 gtggcgcgga tgttgcgctc gattgttctg gtaatgccgc aggtcgcttg ctggcactgc
    26941 aatccaccgc tgactgggga cgggtggttt acattggtga aaccggaaaa gtggaattcg
    27001 aggtcagcgc cgatctgatg caccatcaac ggcggattat cggctcctgg gtgaccagtc
    27061 tgttccatat ggaaaaatgc gcccacgatt tgactgactg gaagctgtgg ccgcgtaacg
    27121 ccattaccca tcgcttctcg ctggaacagg caggtgatgc ctatgcgctg atggcgagcg
    27181 gcaaatgcgg gaaagttgtg attaacttcc cggattaagg agttagcgga tgaccatcta
    27241 taccttatcc cacgggtcgc taaagctgga cgtttctgac cagggcggcg taatcgaagg
    27301 cttctggcgc gatacgacgc cgctcttacg tcctggtaaa aaaagcggtg tggcaaccga
    27361 tgcctcatgt tttccgctgg tgccatttgc aaaccgggtg agcggcaacc gttttgtctg
    27421 gcaggggcgt gagtatcaac tgcaaccgaa tgttgagtgg gatgcgcact atctacacgg
    27481 cgatggctgg ctgggcgagt ggcaatgtgt ctcgcacagc gatgatagtt tgtgtctggt
    27541 gtatgaacat cgcagcggtg tctatcacta tcgggtaagt caggcgtttc atttaacggc
    27601 ggatacgctg acggtgacgc tctctgtcac caatcaaggg gcagagacgc tgccatttgg
    27661 taccggctgg catccttatt ttccgttgtc accacaaacg cggattcagg cgcaggcgag
    27721 cggttactgg ctggaacggg agcagtggct ggcgggtgag ttttgcgagc agctaccgca
    27781 ggaactggat tttaaccagc tcgcgccgtt gccgcgccag tgggtgaaca atggttttgc
    27841 cggatggaat ggtcaggccc gcatcgagca gccgcaggaa ggttatgcca tcatcatgga
    27901 aacgacgcca cctgcaccgt gttatttcat ctttgtttcc gaccctgcgt ttgataaagg
    27961 atatgcgttt gatttcttct gtctggaacc gatgagccat gcgccggatg atcatcatcg
    28021 cccggaaggg ggtgatctca ttgcgctagc accaggggaa tcaacaactt cagagatgtc
    28081 gttgcgggta gagtggctgt aatttattaa atgccggata aggcgttcac gccgcatccg
    28141 gcatctttcc taacgccgat cgagagaaat tgcccccggc ccggtaattg ccaatagcaa
    28201 gaacgcgcca gcgatactga cattcttcca gaaattaatc atatttggcc caaccgcatc
    28261 gccggtcata tcccagtaat ggtgaccaat caccgccgta cccagcgtgt agaaaataaa
    28321 cagcaccgcc agcggacggg tgaaaaagcc aagcacgatt aatatcgcgg cgggcacttc
    28381 cataactacc gcaataatcg ctgccagcat cggcattggc gcgcccaacg aggccatata
    28441 ttggaccgta ccgtcaaagc ccatcatttt gggaaaacca aaaataatga aaattaagac
    28501 cacggcgata cgggcaatta ataacaaaac ggggcgggca gctccaaaat caaaataacg
//
LOCUS       DAJVQG010000038        28407 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJVQG010000038 DAJVQG010000000
VERSION     DAJVQG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.038"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(301..507)
                     /locus_tag="OQM83_001502"
     CDS             complement(301..507)
                     /locus_tag="OQM83_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCS1519793.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            820..909
                     /gene="kdpF"
                     /locus_tag="OQM83_001503"
     CDS             820..909
                     /gene="kdpF"
                     /locus_tag="OQM83_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCS1519794.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            909..2582
                     /gene="kdpA"
                     /locus_tag="OQM83_001504"
     CDS             909..2582
                     /gene="kdpA"
                     /locus_tag="OQM83_001504"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCS1519795.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2605..4653
                     /gene="kdpB"
                     /locus_tag="OQM83_001505"
     CDS             2605..4653
                     /gene="kdpB"
                     /locus_tag="OQM83_001505"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCS1519796.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4662..5234
                     /gene="kdpC"
                     /locus_tag="OQM83_001506"
     CDS             4662..5234
                     /gene="kdpC"
                     /locus_tag="OQM83_001506"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCS1519797.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5227..7911
                     /gene="kdpD"
                     /locus_tag="OQM83_001507"
     CDS             5227..7911
                     /gene="kdpD"
                     /locus_tag="OQM83_001507"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HCS1519798.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7908..8585
                     /gene="kdpE"
                     /locus_tag="OQM83_001508"
     CDS             7908..8585
                     /gene="kdpE"
                     /locus_tag="OQM83_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCS1519799.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8860..8964
                     /locus_tag="OQM83_001509"
     CDS             8860..8964
                     /locus_tag="OQM83_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCS1519800.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9275..11473
                     /gene="speF"
                     /locus_tag="OQM83_001510"
     CDS             9275..11473
                     /gene="speF"
                     /locus_tag="OQM83_001510"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCS1519801.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11470..12789
                     /gene="potE"
                     /locus_tag="OQM83_001511"
     CDS             11470..12789
                     /gene="potE"
                     /locus_tag="OQM83_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCS1519802.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12845..14485)
                     /gene="pgm"
                     /locus_tag="OQM83_001512"
     CDS             complement(12845..14485)
                     /gene="pgm"
                     /locus_tag="OQM83_001512"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCS1519803.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14511..15056)
                     /gene="seqA"
                     /locus_tag="OQM83_001513"
     CDS             complement(14511..15056)
                     /gene="seqA"
                     /locus_tag="OQM83_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCS1519804.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15241..16005
                     /gene="ybfF"
                     /locus_tag="OQM83_001514"
     CDS             15241..16005
                     /gene="ybfF"
                     /locus_tag="OQM83_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1519805.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16145..16438
                     /gene="ybfE"
                     /locus_tag="OQM83_001515"
     CDS             16145..16438
                     /gene="ybfE"
                     /locus_tag="OQM83_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCS1519806.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16578..17108
                     /gene="fldA"
                     /locus_tag="OQM83_001516"
     CDS             16578..17108
                     /gene="fldA"
                     /locus_tag="OQM83_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCS1519807.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17318..17404
                     /gene="uof"
                     /locus_tag="OQM83_001517"
     CDS             17318..17404
                     /gene="uof"
                     /locus_tag="OQM83_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCS1519808.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17397..17843
                     /gene="fur"
                     /locus_tag="OQM83_001518"
     CDS             17397..17843
                     /gene="fur"
                     /locus_tag="OQM83_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCS1519809.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(17928..18254)
                     /locus_tag="OQM83_001519"
     CDS             complement(17928..18254)
                     /locus_tag="OQM83_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1519810.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQYLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18304..19710)
                     /gene="chiP"
                     /locus_tag="OQM83_001520"
                     /pseudo
     CDS             complement(18304..19710)
                     /gene="chiP"
                     /locus_tag="OQM83_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
     gene            complement(20288..21952)
                     /gene="glnS"
                     /locus_tag="OQM83_001521"
     CDS             complement(20288..21952)
                     /gene="glnS"
                     /locus_tag="OQM83_001521"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCS1519811.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22212..24158)
                     /gene="nagE"
                     /locus_tag="OQM83_001522"
     CDS             complement(22212..24158)
                     /gene="nagE"
                     /locus_tag="OQM83_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCS1519812.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            24491..25291
                     /gene="nagB"
                     /locus_tag="OQM83_001523"
     CDS             24491..25291
                     /gene="nagB"
                     /locus_tag="OQM83_001523"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCS1519813.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25351..26499
                     /gene="nagA"
                     /locus_tag="OQM83_001524"
     CDS             25351..26499
                     /gene="nagA"
                     /locus_tag="OQM83_001524"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCS1519814.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            26508..27728
                     /gene="nagC"
                     /locus_tag="OQM83_001525"
                     /gene_synonym="nagR"
     CDS             26508..27728
                     /gene="nagC"
                     /locus_tag="OQM83_001525"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCS1519815.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27776..>28407
                     /gene="nagD"
                     /locus_tag="OQM83_001526"
     CDS             27776..>28407
                     /gene="nagD"
                     /locus_tag="OQM83_001526"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCS1519816.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAG"
BASE COUNT         6664 a         7231 c         7451 g         7061 t
ORIGIN      
        1 acaatgctac cgccatactg cgtcatgtcg ccctgacgcg ccgccggttt tccgctcatt
       61 tctcatcctt gttttatcgg gttatttgtc cgctgacagg caagacccta cagatttata
      121 agaaagcctt aagtgagata tccgtcagat agaaacaatt aggaaaatat ttatatatat
      181 ttcatgaata tatgtttcga ttggtataac ggtgtctaaa tattcctctg aacatcttcc
      241 tgatacaaaa aaccgccagc gtttccgctg gcggttttca atgtattcag aactcagcaa
      301 tcagtaaaaa tcaccggttg ccttttcggc ctgatccatc cacaccggtt tatcgctggt
      361 tttcgaccag acgcggtgca ggtagctgta aaagcgggcg cggtctttcc agaaaagcat
      421 gaaaggcagc gccagaacgc ccgctgcaac cgcatatgta cggcgtaaaa agataagcca
      481 tgcaggatat tctctgtaga gttccatact tttctccccc ttttgtatct acccggtgaa
      541 tagcaccgga aaaatgaatt tgtttatctg atgaaaatag taccgccttt tgtgtaattt
      601 tactactcat ccgaccactt atttttgctt attgatggtt tatttacatt cactctgtaa
      661 ttaagttaca caaaagttaa attaatacta aacattagtt aaatcatggc ttttgccatt
      721 tttatacttt ttttacaccc cgcccgcaga tttttgcgaa atctttgcag ccagaattct
      781 actcttccgg tatcactttt aggccactgg aggtgcactg tgagtgcagg cgtgatagcc
      841 ggcgtgttgc tggtgttttt attactgggt tatttggttt atgccctgat caatgcggag
      901 gcgttctgat ggctgcgcaa gggttcttac tgattgccac gtttttactg gtattaatgg
      961 tgctggcgcg tcctttaggc agcgggctgg cgcggctgat taatgacatt cctcttccag
     1021 gtacagcggg cgttgaacgt atactctttc gtttacctgg cgtctctgac catgagatga
     1081 actggaagca atatctttgt gccattctcg gcctgaacat gctggggctg gcggtgctgt
     1141 tttttatgtt gctcggtcag cactatctgc cgcttaatcc acagcagttg ccggggctgt
     1201 cgtgggatct ggcgctgaat accgccgtca gctttgtcac gaataccaac tggcaatctt
     1261 atagcggtga aaccacgttg agctatttca gccagatggc aggcttaacg gtacaaaact
     1321 ttctttctgc cgccagcggg attgcggtga tttttgcctt catccgtgcg tttactcgcc
     1381 agagcatgag cacactcggg aatgcctggg tcgatctgct acgcatcacg ttatgggtgc
     1441 tagtccctgt ggcgttgttg atcgcactgt tttttattca acaaggtgcg ctgcaaaact
     1501 ttctgcccta tcaggcagtg aataccgttg aaggagcgca acagctgtta cccatgggac
     1561 cagtagcttc tcaggaagcg atcaagatgc tcggtactaa cggcggtggc ttctttaatg
     1621 ccaactcgtc gcatccgttt gaaaacccaa ccgcactgac caacttcgtg cagatgctgg
     1681 cgatcttctt gatcccaaca gcgctgtgct ttgcctttgg tgaagtgacg ggcgatcgcc
     1741 gccaggggcg catgttgctg tgggcgatgt cagtgatttt tgtcatctgc gtaggcgtgg
     1801 ttatgtgggc agaagttcag ggtaatcctc atctgctggc gctcggcgca gacagcagca
     1861 tcaatatgga aggtaaagag agccgtttcg gcgtgctggt cagtagcctg tttgcggtcg
     1921 tgactacggc ggcgtcctgt ggcgcggtga ttgcgatgca tgattcgttt accgctctcg
     1981 gcggcatggt gccgatgtgg ctaatgcaaa ttggtgaagt ggtgttcggc ggtgtcggtt
     2041 ctggtcttta cggcatgatg ctgtttgtcc tgctggcggt gtttattgcc gggctgatga
     2101 ttggtcgtac accggaatat ctgggtaaaa aaatcgacgt acgcgagatg aaactgactg
     2161 cactggcgat tctggttact ccaacactgg tactgatggg cgcggcgttg gcgatgatga
     2221 ccgacgccgg acgtagcgcc atgcttaacc ctggcccgca tggttttagc gaagtgctgt
     2281 acgccgtgtc gtccgccgct aacaacaacg gcagcgcctt tgccggatta agcgccaact
     2341 ctccgttctg gaactgttta ctggcgttct gcatgtttgt cggtcgcttc ggggtgatta
     2401 tcccggtgat ggcaattgcc ggttcgctgg tgagtaaaaa gagccaaccc gccagctccg
     2461 gcacgctgcc aacgcacggc ccgctgtttg ttggcctgtt aatcggcacc gtgttgctgg
     2521 ttggcgcact gacctttatc cctgccctgg cgcttggtcc ggtggcggaa tatctctcct
     2581 gatgatattg agcgagcact gaatatgagt cgtaaacaac tggcgctatt cgaaccaaca
     2641 cttgtcgttc aggcgctgaa agaagcggtg aaaaaattaa acccgcaggc gcaatggcgc
     2701 aatccggtga tgtttatcgt ctggatcggc agtctgctga ccacctgtat tagcatcgcg
     2761 atggcaagcg gtgcgatgcc tggcaatgcg ctgtttagcg cggcaattag cggctggctg
     2821 tgggtcaccg tactgttcgc taatttcgcc gaggcgctgg cagaaggccg cagtaaagcg
     2881 caggccaaca gtcttaaagg ggtgaaaaaa actgcctttg cccgcaagct gcgtgagccg
     2941 aaatatggcg ctgcggcgga caaagttcct gccgatcaac tgcgtaaagg tgacatcgtg
     3001 ctggtagaag ctggcgatat tatcccctgc gatggtgaag tcattgaagg cggcgcatcg
     3061 gtcgatgaaa gcgccatcac cggggaatcg gcaccggtga tccgtgaatc cggcggcgat
     3121 tttgcctccg tcaccggcgg cacgcgtatt ctttctgact ggctggtgat tgagtgtagc
     3181 gttaaccccg gtgagacatt tctggatcgg atgatcgcga tggtggaagg cgcacagcga
     3241 cgcaaaacac cgaacgagat tgccctgact atcctgctga ttgctctgac catcgtcttt
     3301 ttactggcaa ccgccacgct gtggccgttt tccgcgtggg gcggtaatgc cgtgagtgta
     3361 acggtactgg tggcgctgct ggtctgtctg atcccaacca ctattggcgg cctgttgtca
     3421 gcgatcggcg tcgccgggat gagtcggatg ctgggcgcga atgtgattgc caccagcgga
     3481 cgtgcagttg aagcggcagg tgacgttgac gttctgctac tggataaaac cggcaccatc
     3541 acactcggta accgtcaggc gtcggagttt atccccgcgc agggcgtgga tgaaaaaacg
     3601 ctggctgacg ccgcacaact ggcttcgctg gctgatgaaa cgccggaagg tcgcagtatt
     3661 gtgatcctcg ccaagcaacg ttttaacctg cgcgagcgcg atgtgcagtc gctccatgcc
     3721 acctttgtac cgtttactgc gcaaagccgg atgagcggga tcaacatcga caaccgcatg
     3781 atccgtaaag gttctgtcga tgccattcgc cgccatattg aggctaacgg cggtcacttc
     3841 cctgccgatg ttgatcaaaa agtcgatcag gttgcgcgtc agggagccac gccgctggtg
     3901 gtggtggaag gttctcgtgt gctgggcgtt attgcgctga aagatatcgt caaaggcggt
     3961 attaaagagc gcttcgccca gctgcgcaaa atgggcatta aaacggtgat gattaccggc
     4021 gataaccgtc tgactgccgc cgcgatagct gcggaagcgg gtgtcgatga ttttctcgcc
     4081 gaagcaacac cggaggccaa gctggcattg atccgtcagt atcaggcgga aggtcgtttg
     4141 gtagcgatga ccggcgacgg caccaacgat gctccggcgc tggcgcaggc agatgtcgcg
     4201 gtggcgatga actccggcac ccaggcggcg aaagaggcgg gcaatatggt ggatctcgac
     4261 tctaacccga ccaagttgat cgaggtggtg cacattggca aacagatgct gatgacccgt
     4321 ggctcgctga ccaccttcag cattgccaac gatgtggcga aatacttcgc cattattccg
     4381 gcggcgttcg cggcaacata tccgcagtta aatgcgctga acatcatgcg cctgcattcg
     4441 cccgactccg ccattctcag tgcggtcatt ttcaacgcct tgattatcgt ctttttgatt
     4501 cctctggcgt taaaaggcgt gagttataaa ccgcttaccg cttctgccat gttgcgccgt
     4561 aacttatgga tttacggtct gggtgggctg ctggtgccgt ttattggtat caaagtcatt
     4621 gatttactgc tgaccgtttg cggtctggtg tgaggtttac catgagtgga ttacgtccgg
     4681 cattatcgac atttctcttt ctgttattga ttactggcgg cgtttacccg ctgctgacca
     4741 ccgcactggg gcaatggtgg tttccctggc aggccaacgg ttcgttgatc cgtgaaggtg
     4801 atacggtgcg tggctcggca ttaatcgggc agaattttac cggcaacggc tattttcatg
     4861 gtcgcccgtc agcaacggct gaaatgccct ataatccaca ggcttctggc gggagcaatc
     4921 tggcggtcag taaccctgag ctggataaac taatagccgc acgcgttgct gcattacggg
     4981 ccgctaaccc ggatgccagc gcgagcattc cggttgaact ggtgacggca tcggcaagcg
     5041 ggctggacaa taatatcacc ccgcaagcgg cggcctggca gatcccacgc atcgcgaaag
     5101 cgcgtaatct cagcgttgaa cagctcacgc agctgatcgc aaaatacagc caacaaccgc
     5161 tggtgaaata tatcggccag ccggtcgtca acattgtcga actcaatctg gcgctggata
     5221 agcttgatga ataacgaacc cttacgtccc gaccccgatc gtctgctgga acaaactgcc
     5281 gcgccgcatc gggggaagct gaaagttttc ttcggtgcct gtgcaggcgt cgggaagacc
     5341 tgggcgatgc tggcagaagc ccagcgactg cgggcgcaag ggctggatat tgtggttggc
     5401 gtggtagaaa cccacgggcg aaaagatacc gccgcaatgc tggaagggct ggctgttctg
     5461 ccgccaaaac gcctggcgta ccgtgggcgg cacatctgcg agtttgacct cgatgccgcc
     5521 ctcgcccgcc gtccggcgct gatcttaatg gacgaactgg cgcacagtaa tgcgccaggt
     5581 tctcgtcacc ctaaacgctg gcaggacatc gaagagctgc tggaagcagg cattgatgtt
     5641 ttcactaccg tcaacgttca gcatctggaa agtctgaatg atgtggtcag cggcgtcacc
     5701 ggaattcagg tacgggaaac cgtgcccgat ccttttttcg atgccgccga cgacgtggtt
     5761 ctggtggact tgcccccgga cgatctgcgc cagcggctga aagaaggcaa agtctatatt
     5821 gccgggcagg cggagcgcgc cattgaacat tttttccgca aaggcaatct gatcgccctg
     5881 cgcgaactgg cactgcgccg tactgccgat cgcgttgatg agcaaatgcg cgcctggcgg
     5941 gggcatccta gcgaagagaa agtctggcac acgcgcgacg cgatcctttt atgcatcggc
     6001 cataacaccg gtagtgagaa gctggtccgc gcggcggcgc ggctggcgtc acggctgggt
     6061 agcgtctggc acgcggtgta tgttgaaacc cctgccctgc atcgcttacc ggagaaaaaa
     6121 cgtcgtgcaa ttctcagcgc cttacgtctg gcgcaggaac tgggcgcgga gacggcaaca
     6181 ctttctgatc cagcggaaga gaaagcggta gtgcgttatg cccgtgaaca taatctcggc
     6241 aagattattc tcggtcgccc ggcctcgcgc cgctggtggc gtcgggaaac gtttgctgac
     6301 cgactggcgc gcatcgcccc cgatctcgat caggtgctgg tcgcgcttga tgaaccaccc
     6361 gcccgcacga ttaacaacgc gccggatagc cgctctttta aagacaagtg gcgggtacag
     6421 attcagggat gcgtggttgc cgccgcgtta tgcgccgtta tcaccttaat tgccatgcag
     6481 tggctgatgg cgtttgatgc cgccaacctg gtgatgctgt atctgcttgg cgtggtggtg
     6541 gtggcgctat tttatggacg ctggccttcg gtggttgcca ccgtcattaa tgtggtgagt
     6601 ttcgatctct tttttatcgc cccacgcggc acgctcgccg tctctgatgt gcaatatctg
     6661 ctgaccttcg cggtgatgtt aaccgtcggg ctggtgatcg ggaaccttac tgctggcgtg
     6721 cgttatcagg cgcgggtagc ccgttaccgc gagcaacgca cacggcactt atatgaaatg
     6781 tcgaaagctc tggcggtggg ccgcagtccg caggacatcg ccgccaccag cgaacaattt
     6841 attgcctcca cgtttcatgc ccgcagtcag gtgttgttgc ccgatgacaa cggtaaattg
     6901 cagccgttaa cacatccgca aggaatgacg ccatgggacg atgccatcgc gcagtggagt
     6961 tttgataaag gcctgcctgc gggcgcgggc accgacacgt tacccggtgt accgtaccag
     7021 attttgccgc taaaaagcgg cgagaaaacc tacgggctgg tggtggtgga gccggggaat
     7081 ctgcgccagt tgatgatccc ggaacagcag cgcctgctgg agacgtttac gctgttagtc
     7141 gccaatgccc ttgagcggct gacgctaacc gccagcgaag aacaggcgcg gatggcaagc
     7201 gaacgtgaac agatccgcaa cgccctgctg gcagcgcttt cgcatgattt acgcacgccg
     7261 cttacggtgc tgtttggtca ggcagaaatc ttaacgctcg atctggcaag cgaaggatca
     7321 ccccacgccc gccaggccag cgagattcgt cagcatgtgc tgaacactac ccgactggtg
     7381 aataatctac tggatatggc gcgaattcag tccggcggct ttaatttgaa gaaagagtgg
     7441 ttaacgctgg aagaagtagt cggcagcgcg ctgcaaatgc tggaaccggg tttatcgtcg
     7501 cccatcaatc tttctctgcc agaaccgctg accttaatcc acgttgacgg gccactcttt
     7561 gaacgggtgc tgattaatct gctggagaac gcggtgaaat atgcgggtgc gcaggccgaa
     7621 attggtatcg atgcccactt tgagggcgaa aatctacaac tggatgtctg ggataacggc
     7681 cccggtcttc cgccaggcca ggagcagacg atatttgata agtttgctcg cgggaataaa
     7741 gagtcggcgg tgccgggtgt cgggcttggg ttggcaattt gtcgggcgat tgtggatgta
     7801 cacggcggca ccattaccgc gttcaaccga ccggaaggtg gtgcctgttt tcgtgttaca
     7861 cttccccagc aaaccgctcc tgaacttgaa gactttcatg aggatatgtg acaaacgttc
     7921 tgattgttga agatgaacag gctattcgtc gctttctgcg cacggcgctg gagggcgacg
     7981 ggatgcgcgt ctttgaggcc gaaactctgc aacgcggctt gctggaagcg gcaacccgta
     8041 agccagattt gattattctc gatctcggcc tgcccgatgg tgatgggatt gagtttatcc
     8101 gcgacctgcg ccagtggagc ccggtgccgg tgattgtgct ttccgcacgc agcgaagaga
     8161 gcgacaagat cgccgcgctg gatgccggag cggatgatta tctgagtaag ccgtttggca
     8221 ttggcgaatt gcaggcccgt ctgcgcgtcg ctttacgccg ccactccgcc accgccgcgc
     8281 ccgatccgct ggtgaaattt tccgatgtta ccgtcgattt agccgcccgc gtgattcacc
     8341 ggggtgagga agaggtgcat ctcacaccaa ttgagttccg cctgctggcg gtgctgctca
     8401 acaatgccgg aaaagtactc acccagcgcc agctccttaa ccaggtgtgg gggccaaacg
     8461 cggtcgaaca cagtcactat ttgcgtattt atatgggaca tctgcgacaa aaactggaac
     8521 aggatccggc ccggcctcgc cacttcatta ctgaaaccgg tattggatat cggtttatgc
     8581 tttgaattgt tattcaataa attcgcttaa atagtgatta atttattatt aaacctgcgc
     8641 cgaatattat tatttggcag gtttaatttc ttaacgcaaa tataaaacac gaaattacaa
     8701 catttaagta acaaaacagc atctaatatg gcgatattca taattaatga tttattatct
     8761 ggggtatgat cgttttttgt tgatcttctt cacagattat agccatttca tggatagaat
     8821 aactctacct tcaactgaca cagcaagagg taaaggtaaa tggaaaataa taaccgctta
     8881 atgcctcata taaggcggac aacccatatc atgaagtttg cccatcgtaa tagttttgac
     8941 tttcatttct ttaatgcccg ctagtcttct gactaaaggg caccccaacg tacaggtctc
     9001 cctgacttta agcattacag gttaatacct gtattcctcg gtgctcatat actgctaacc
     9061 cttttaaact ctaaataatt cgagtcgcag cacttgcaac ttgaggtatg acgagtatag
     9121 ccagttaccg ggctggtctg ggttattgca tctgcaaaaa gcaaactact gatttattta
     9181 tcagcggtgg agctttgctt tttttccggc gtgatcgatt tctcctttga gaaattgagg
     9241 acctgctatt acctgaaata aagagatgaa taaaatgtca gaattaaaaa ttgccgttag
     9301 ccgttcttgc ccggattgtt tttccactca tcgtgcatgc gtgaatattg acgaaagtaa
     9361 ttatattgac gttgccgcca ttattttatc agtcaatgat gttgaacgtg gaaaactcga
     9421 tgaaatagac gctactggct atggcattcc tgtttttatt gcaacggaaa atgaagaacg
     9481 tgtaccagca gaatatctgc cacgtatttc tggtgtcttc gagcattgcg aatcacggaa
     9541 agagttttat ggacgtcagt tagaaaccgc tgctagccat tatgaaacgc aactgcgccc
     9601 gcccttcttc cgcgcactgg tcgattatgt caatcagggc aatagcgctt ttgactgccc
     9661 gggtcatcag ggaggtgagt ttttccgtcg tcatccggca gggaatcagt tcgtggagta
     9721 cttcggagaa acgctgttcc gttccgactt atgcaacgca gacgtagcaa tgggcgatct
     9781 gctgattcac gaaggcgcgc catgcattgc acagcaacat gcggcaaaag tgtttaatgc
     9841 cgataaaaca tacttcgttc taaatggcac ttcatcttct aacaaagtgg ttttaaacgc
     9901 cctgctaaca ccgggtgatc tggtgctgtt tgatcgcaat aaccacaaat ctaaccacca
     9961 cggagcgttg ctacaggctg gtgcaacacc ggtttatctg gaaacggcac gtaacccgta
    10021 tggctttatc ggtggcattg atgcgcactg ttttgaagaa agttacctgc gtgagctgat
    10081 cgcggaagtc gcaccgcagc gggcaaaaga ggctcgtcct ttccgcctcg ctgtgattca
    10141 gttaggcacc tacgacggta cgatttataa cgcccgccag gtggtggata aaattggtca
    10201 tctgtgtgac tacattctgt ttgactccgc atgggttggc tatgagcaat ttatcccgat
    10261 gatggctgac tgttcgccgc tgttgctgga tcttaatgag aacgatccgg gtattctggt
    10321 tacgcaatcc gtacataaac agcaggctgg tttttctcaa acttcacaaa tccataaaaa
    10381 agacagccac atcaaagggc aacagcgtta tgtaccgcac aaacgcatga acaacgcctt
    10441 tatgatgcac gcctccacca gcccgttcta tccgctgttt gccgcactgg atatcaacgc
    10501 caaaatgcat gaaggtgtca gcggtcgtaa tatgtggatg gattgtgtgg taaatggcat
    10561 taatgcccgc aaactgatcc tcgataactg tcagcatatt cgtccgttcg tacctgaact
    10621 ggtggatggt aaaccctggc agtcgtatga aacagcgcaa attgcggttg atctgcgctt
    10681 cttccagttt gtaccagggg aacactggca ttcttttgaa ggctatgcag agaatcaata
    10741 ctttgtcgat ccttgcaaac tgttgctgac aaccccaggt attgatgcac gtaacggcga
    10801 atatgaagcg ttcggtgtac ccgcgacgat tcttgctaac ttcctgcgcg aaaatggcgt
    10861 agtgccggaa aaatgcgatc ttaactccat cctcttcctg ctgactccgg cagaagatat
    10921 ggccaaactt cagcaacttg ttgccctgct ggtacgcttc gaaaaactgc ttgagtccga
    10981 cgcgccatta gcagaagtgc tgccttccat ctacaaacag catgaagagc gctacgccgg
    11041 ttataccctg cgtcagttgt gccaggaaat gcatgatttg tatgcccgcc acaacgtgaa
    11101 acaactgcaa aaagagatgt tccgcaaagc gcacttccca cacgtcagca tgaatccgca
    11161 agaagccaac tacgcctatt tacgcggtga agtggagctg gttcgtctgc cggatgccga
    11221 aggtcgtatc gctgccgaag gggcgcttcc ttatcctccg ggtgtgctgt gtgttgttcc
    11281 gggtgaaatc tggggtggtg ctgttttgcg ttacttcagc gctctggaag aagggatcaa
    11341 cctgctgcca ggttttgcac cggagctgca gggtgtctat atcgaagaac atgatggtcg
    11401 taagcaagtt tggtgctatg tcatcaagcc tcgtgatgcg caaagcaccc tgttgaaagg
    11461 ggaaaaatta tgagtcaggc taaatcgaac aaaatgggcg tcgttcagtt aaccatactg
    11521 acgatggtca acatgatggg ctccggtatc atcatgctgc cgacaaagct tgccgaagtt
    11581 gggacaattt caattatctc ctggctggtg acagccgtag gctcaatggc actggcatgg
    11641 gcattcgcaa aatgcggtat gttcagccgt aaatcaggtg gtatgggcgg ttatgccgag
    11701 tatgctttcg gtaaatccgg taactttatg gcgaactata cctacggcgt ctcactgctg
    11761 attgctaacg tcgcgattgc tatttcggcg gttggttacg gcaccgaact gctcggcgcg
    11821 agtttgtcgc cagtgcagat tggtcttgcg accatcgggg tgctgtggat ttgtaccgtg
    11881 gctaactttg gtggtgcgcg cattaccggg caaatcagta gcattaccgt gtggggggtc
    11941 attattccgg tggtaggcct gtgcattatc ggctggttct ggtttagccc gacgctttac
    12001 gttgattcct ggaatccgca tcatgcaccg ttcttcagtg cagtaggttc ttccatcgct
    12061 atgacgctgt gggcttttct cggtctggag tctgcgtgtg cgaatactga tgtagtggaa
    12121 aacccggaac gtaatgtgcc aatcgcggta ctcggcggta cgttaggtgc ggcggtgatt
    12181 tatatcgtct ccaccaacgt gattgcaggg attgtgccta atatggagct ggcaaattca
    12241 acggcaccat ttggcctggc cttcgcacag atgttcacac cagaagtggg taaagtcatt
    12301 atggcgctga tggtgatgtc ctgctgcggt tcgctgcttg gctggcagtt caccattgcc
    12361 caggtgttta agtcttcatc tgatgaaggc tacttcccta aaattttctc ccgtgtaacc
    12421 aaagtggatg caccggtgca gggaatgttg accattgtga ttatccagag cgggttggcg
    12481 ctgatgacga ttagcccgtc gctaaacagt cagttcaacg tgctggttaa cctggccgtg
    12541 gtgaccaata tcattccgta tattctgtca atggcggcat tagtcattat tcagaaggtc
    12601 gctaatgtgc ccccctcaaa agcgaaagtt gcaaactttg ttgcttttgt tggcgcgatg
    12661 tatagctttt atgcgctgta ctcatccggg gaagaagcca tgctgtacgg ttccatcgtg
    12721 accttccttg gttggacact gtatggtctg gtatcaccac gctttgaact gaaaaataaa
    12781 cacggttaaa aaagcaaagt aaaaaagggc gatcttgcga ccgccctttt tttattaaat
    12841 gtgtttacgc gtttttcaga acttcgctaa caatctcaac cgcttctttc tcaatctgct
    12901 tgcgatgttc ttcaccgagg aagctttcgc agtagatctt atatgcgtct tccgtgcctg
    12961 acggacgcgc ggcgaaccag ccgttgtcag tcatcacttt cagaccgccg atagaagcac
    13021 cattgcccgg tgccgccgtc aggcgcgcgg tgatcgggtc gcctgccagg gtgctggcgc
    13081 tcaccatttc cggagacagc ttagacagcg ccgctttttg tgcggaagtc gcagctgcct
    13141 gcaaacggtt gtagctcggc gcaccaaagc gttttgccag ttcgttgtag tgttcctgcg
    13201 ggttcttacc ggtgacagcg gtgatttccg ccgccagcag acacatgatg atgccgtctt
    13261 tgtcggtgga ccacggcgtg ccgtcgaaac gcaggaagga agcccctgcg ctctcttcgc
    13321 cgccaaagcc gaagctgccg tcgaacagac catcaacaaa ccatttgaaa cctaccggga
    13381 cttctaccag cttgcggccc aagtcattga ccacacggtc gatcatcgca gaagaaacca
    13441 gcgttttacc gacagcaaca tctttgcccc actgcggacg atgctggaac aggtagttga
    13501 tcgccaccgc caggtagtgg ttcggattca tcaaacctgc cggagtgacg ataccgtgac
    13561 ggtcataatc cgggtcgtta gcaaacgcca gatcgaactt atcacgcaat gccagcaggc
    13621 ccgccatcgc acactcggag gagcagtcca tacggatcgc gccgtcttta tcgaggtgca
    13681 taaagcggaa ggtttgatcg acctgatcgt taacgatggt caggttgagg ttgtaatact
    13741 cgccaattcg cttccagtat tcgataccgg aaccacccag cggatcaacg cccagcgtca
    13801 ggcccgtttt ctgaatcgcc gccatatcaa cgatatcggc cagaccttcc acaaactgct
    13861 gcaccagatc ctgctctttc acatgaccgg atgccatcgc ttcgtcgagg gagatacgct
    13921 tcacgccttt caggccatcg gccatcagtg cgttggcacg atcttccacc actttagtga
    13981 cgttggtatc agccgggcca ccatttggcg gattgtactt gataccacca tcttccggcg
    14041 ggttatggga gggtgtaatc acgataccgt ctgccagcgg gccacctttt ttattatgaa
    14101 ccaggatggc attggaaacg gcaggcgtcg gagtgaagcc attgttttcc tgcacaatga
    14161 catcaacgcc gttcgctgcc agcacttcca gtacggaaat gaacgcaggc tcggacaggg
    14221 cgtgagtatc tttacccaca tagcaagggc cagtgatgcc gtttttcgca cgttcttcag
    14281 caattgcctg agcgatcgcc agaatgtgcg gctcgttaaa gctgtggcgc gctgcactgc
    14341 cacggtgacc ggaagtaccg aatttcaccg cgtgctccgc attccctgct tctggtttca
    14401 gtacataata ttgcgccgtc agttgggcga cgttaatcaa atcactctgt tgtgcaggtt
    14461 ggcctgcacg attgtggatt gccattgctt tgtcctttgt ctgcaacgtt ttagatagtt
    14521 ccgcaaacct tctcaatcaa ttccgccggg aattgcatcg actgcatgat gtgttcgatc
    14581 atgctgcatt tacggccggt gttggtgttg gtgatcaccc aatacggcgt gcctggcaca
    14641 tgtttcggct tggtctgatt accatttttc agcagcgttt gttcatctgc cgcaaagtaa
    14701 acgcgtgtac gaccgtgcaa cgattccgtt gcttcggcaa acgcctgggc gtcaagagaa
    14761 tatagtgtag acaacagcag cataaagcga ttgaccgctc gcttttgctc tgcgtattcg
    14821 tcagaaagca gaagttcacg cattgcgcga accttgtctt taatcgtttt gaccggcttc
    14881 gcttcgacga tagcaggtga cgcaacgcga acctctttcg tcaccggagc agcaggctgt
    14941 gatgcggcgg aaaatttcaa catacgccgt aaaatgtcgg atgcgctctc gccgatatgc
    15001 ttagtgtggc tggcaatata gctgtagagt tcatcatcaa cttcaatcgt tttcatctta
    15061 atccagtaca gtgtcttagc tgaatacaag tcgccaagag tatatgaata aatcccggca
    15121 gcggatagcg tcaagcctgg cttgcagaaa aactcggaat aaaccttatt tgttgcagcc
    15181 ctgtggcaca atggtcaaca taatacccta acccgacaac gcgaaaaaaa gaactttgcc
    15241 atgaaattga atatccgcgc gcaaactgca caaaaccagc acaataattc tcccatcgtt
    15301 cttgtccatg gtctgtttgg tagcctcgac aaccttggcg tactggctcg cgatctggta
    15361 aacgatcaca atatcatcca ggttgatatg cgtaaccacg gtctttcacc gagagatccg
    15421 gtaatgaatt acccggcgat ggcgcaggat cttgttgata ctctggatgc acagcagatc
    15481 gacaaagcaa catttatcgg tcactccatg ggcggtaaag cggtaatggc acttacggca
    15541 ctggcccgcg atcgcatcga taaactggtg gcgatcgata tcgcaccggt cgactatcac
    15601 gtacgccgtc atgatgagat tttcgcggct atcaacgcgg tcagtgaatc ggacgcacaa
    15661 actcgccagc aagcagcagc aataatgcac cagcatctta atgaagaagg ggtgattcag
    15721 tttctgttga aatcttttgt tgacggggag tggcgcttta acgtgccggt attgtgggat
    15781 cagtatccgc atattgtggg ttgggagaaa atcccggcat gggatcaccc tgccctgttt
    15841 atccctggcg gcaattctcc gtatgttagc gagcagtacc gtgatgattt actggcacaa
    15901 tttccacagg cacgggcgca tgtgattgca ggcgcgggtc actgggtcca tgctgaaaaa
    15961 ccggatgcgg tattacgcgc tatccgtcgc tatctcaatg attaacgcaa ctgattaaaa
    16021 acccagcgtc cgcgtcgctt tccgcgcgtg ggcgttggcg tgactcatcg cgctgatgta
    16081 ttatggcgcg ctatccatcc gggcagaggc ctggctgatt gtttcccccg aagtcaccaa
    16141 gatcatggcc aaagaacaaa cggaccgtac gacattagat ctgttcgcgc acgagcgtcg
    16201 accgggacga ccgaaaacta atccgctttc gcgcgatgaa cagctgcgta ttaataaacg
    16261 caaccagcta aaacgcgaca aagtacgtgg ccttaagcgt gtcgaactga agctgaacgc
    16321 ggaagctgtc gaggcgctga acgagctggc ggagtcgcgc aatatgagcc gtagcgaact
    16381 gatcgaagag atgttaatgc agcaactggc ggcgctgcgt agtcagggca tcgtttaaat
    16441 ttccactttc atgtagcaca gtgtgcagtc ctgctcgttt gctgattccg caagactgcc
    16501 tgttctgcta tgattgcctt tatccgtggg caattttcca cccccatttc aataagtttc
    16561 aagaggttat ttcactcatg gctatcactg gcatcttttt cggcagcgac accggtaata
    16621 ccgaaaatat cgcaaaaatg attcaaaaac agcttggtaa agacgttgcc gatgtccatg
    16681 acattgcaaa aagcagcaaa gaagatctgg aagcttatga cattctgctg ctgggcatcc
    16741 caacctggta ttatggcgaa gcgcagtgtg actgggatga cttcttcccg actctcgaag
    16801 agattgattt caacggcaaa ctggttgcgc tgtttggttg tggtgaccag gaagattacg
    16861 ccgaatattt ctgcgacgca ttgggcacca tccgcgacat cattgaaccg cgcggtgcaa
    16921 cgatcgttgg tcactggcca actgcgggct atcatttcga agcatcaaaa ggtctggctg
    16981 atgacgacca ctttgtcggt ctggctatcg acgaagaccg tcagccggaa ctgaccgctg
    17041 aacgtgtaga aaaatgggtt aaacagattt ctgaagagtt gcatctcgac gaaattctca
    17101 atgcctgatg tgatgcggcg tagactcatg tctacgccgt attaatagat aatgccaatc
    17161 aaaataattg ctacaaattt gtaacttttg ctgttgtacc tgtacaatgt cccggtgttc
    17221 aagtggcctt gccgttgtaa atgtaagctg tgccacgttt ttattaacaa tatttgccag
    17281 ggacttgtgg ttttcattta ggcgtggcaa ttctataatg atacgcatta tctcaagagc
    17341 aaattctgtc acttcttcta atgaagtgaa ccgcttagta acaggacaga ttccgcatga
    17401 ctgataacaa taccgcccta aagaaagctg gcctgaaagt aacgcttcct cgtttaaaaa
    17461 tcctagaagt tcttcaggag ccggacaacc atcacgtcag tgcggaagat ttatacaaac
    17521 gtctgatcga tatgggtgaa gaaattggtc tggctacggt atatcgcgta ctgaaccagt
    17581 ttgacgacgc tggtatcgtc acccgccaca attttgaagg cggtaaatcc gtatttgaac
    17641 tgacacagca acatcaccac gatcacctga tctgcctcga ctgcggcaag gttatcgaat
    17701 ttagtgatga ttccatcgaa gcgcgtcagc gtgaaattgc cgcaaaacat ggcattcgcc
    17761 tgactaacca cagtctctat ctttacggtc actgtgccga aggcgattgc cgcgaagatg
    17821 aacatgcgca cgaaggcaaa taagccagcc tgaatgagaa aaagccaacc tgcgggttgg
    17881 cttttttatg caagggaaat aagaaaagcg agagttacag ctctcactta tttgttatta
    17941 ctgcgaattt cctgccaaac tttatcacaa tcggctttca ccgcctgatc atttccggtt
    18001 tgcgttgcac gcagatactg ctgataatcc agtacgcgga cactttcctg gtcggcaaaa
    18061 tgctgatgtt gtttctcttt cttcagcacg tttaacacgc tctggcaggc ttcaattttt
    18121 tctggcgaac cttgtgcggt gttgatacag gcgctgtatg cctctttcag acgactgtct
    18181 tcctgtggcg cggttgattg cgcacaagct accagccccg atgccattat ggcgatgaga
    18241 atcagttttt tcatagcaaa ttcctcaaac gtgcggcagg cacaaaacct gccgcgtcgg
    18301 gcatcagaag atggtgaatg gtgcgattac cataaatttc acgtcacgct catcctggaa
    18361 gatgttgccg taaccaccgc cccagcttgg gatgtcggag tggttgtcgt attcggtgaa
    18421 gtgcagtttg aacatcgtgc ctttggcgcg accgtcctga atggtgtaga ccgcatccag
    18481 gctgtatgca gactcttcaa tagtccggtt tttgtcgtag tacgcatccg gattgctctg
    18541 ctaggtcgca ggtttagcat cccatgcgta aacgtaggaa gcgccgatgg cgaagcctgg
    18601 aagattccag tttttcaggt catacatcgc accgaagaag accgcttttt cgccgttagc
    18661 gttgaagtcg gaacggttat cccaccagat atccaggcgg ccgttggagg aagcgtaggt
    18721 tggagtcata cgttgcagga agtatccctg ctgaccgtca gccttaaccc aggtgccttc
    18781 gaggcgcaaa tccactacgt cagccgcccg gtaaccaaag gtcaacgctt gcagccaggc
    18841 ggtgccgtca taaaggtcgt tgacgctgcg atcgtcaact ttatcgcgcg taccatagaa
    18901 ctggtagctg gtggttaacg ggctaccggc gatatcaaat ttgtagctgg ctttggcaaa
    18961 atattgatcg atataccctt ccgcctgacc aaatgccgct tccagtacga agttattttt
    19021 gaagtcgtat ttcgccccaa gggagtgcag ataatcaact ttggtggttt tatcgttctg
    19081 ataaaactca tccatttcca gatgccacgg cgctttgtat tcgttggtcc acatgtagga
    19141 gaaactcaac gcaccagcat cgccgtaatc aaaattcgcc ccggcttccg ccccctgata
    19201 agtacctggc ataaagctcc agtgcggcgc taacagcgtt tgaccagttg gctgaatgta
    19261 acctgccctc gcccaaaccg gaccgtattt aaatttggcc gcagctttat acaggcttat
    19321 accgcttttg tcgccggacc agtcttcgtc ataggcttta ttactttttg aaaacgcgat
    19381 ttcgttcggg tgggagctgt cgccgttttc cgccatttca atcgccgtaa acgcagcaat
    19441 atcaaggccg aacatatcag cagcataacc ggactgaaaa tcgaggttgg cattccaggt
    19501 ggagtgagaa aggttggttt tgtatttgtc gccgtcggta acatctttac ggtcgcgttc
    19561 acgctgccag taatatatac cgccggttaa ggttgaatcg tcgatgaatc cttcggcgcg
    19621 agcctccggc attgccataa agcccgacat tgctgtaaca ccggcgatag ccagcgccag
    19681 cgtactacgt ttgccactaa acgtacgcat gggttaatcc tctttgacgt ataaattgct
    19741 gcaccaaagg tgaacagcga aaaaataaaa attaaaaaag tagcgttgag aaaagtctcg
    19801 ctgactacag gaacagacta tttttcgcga cgcgaattat caatctcttt ctgtaaccag
    19861 aatgtaagaa catgagcaac cgcacatatt ttgatgattt ttgttacaag gcttaagttt
    19921 gcggttgaaa ggcttgcaga agaataaaag cgtgaaattc cagcaagatg aaagcggtta
    19981 cattttattt aattatgttt tgttatttta aatttatttt atggataaat tgcgaagcgg
    20041 aattaattcg cgaagaaaag cagaaaaaac gccgctgaac gcggcgtttt ggattgtttg
    20101 catatttctt gccggatgcg gcgtgaacgc cttaaccggc ctacaaaagc acgcaaactc
    20161 aatatattgc agagatcatg taggcctgat aagcgtagtg catcaggcaa tttagcgttt
    20221 gaaactgttt gtctgttcct tgccggatgc gacgtaaacg ccccatccgg catagcgaaa
    20281 cttaaaatta ctcgcctact ttcgcccagg tatcacgcag cccaacggtg cggttaaata
    20341 ccggtttttc cgccgtagaa tggcggctat cgaggcagaa gtaaccttca cgctcaaact
    20401 ggaatgcttt acccgcaacc gcatctttca gcgacggttc agcaaagccc tgtttgatca
    20461 ccagcgattc cgggttaatc accgacagga aatcatccgc agcaccaggg ttcggcacgc
    20521 tgaacagacg gtcgtacaga cggatttcaa ccggcagcgc atgtgccgcg ctcacccagt
    20581 gaataacacc tttgacttta cgaccatctg ccggatcttt gcttaaggtg tcggcgtcat
    20641 aagtacagaa gatggtggtg atattgcctt cggcatcttt ctccacgcgt tctgccttaa
    20701 tgacgtaagc attacgcaga cgcacttctt tacccagcac cagacgtttg tactgcttgt
    20761 tagcttcttc gcggaaatcg gcgcgatcga tccaaatctc accgctaaac ggcacctggc
    20821 ggctacccat ttccggtttg ttcggatggt tcggcatggt aaccatttcg ccttcgccct
    20881 gatagttttc gataaccagt ttcaccggat cgataaccgc cattgcgcgc ggggcgtttt
    20941 cgttgagatc ttcacggatg caggattcca gcgacgccat ctcaatggtg ttgtcctgct
    21001 tggtcacgcc gatgcgtttg cagaactcac gaatagaagc agcagtgtaa ccacgacgac
    21061 gcagaccgga aatggtcggc atacgcgggt catcccagcc ttcaacgtgc ttgtcggtca
    21121 ccagcaggtt cagcttacgc ttggacatca cagtgtattc cagattcagg cgcgagaatt
    21181 cgtactggcg cgggtgaaca ggaatggtga tgttgtccag cacccagtcg tacaggcgac
    21241 ggttgtcctg gaactcaagc gtacacagag agtgcgtaat accttccagg gcatcgctga
    21301 tgcagtgggt gaagtcgtac atcgggtaga tgcaccactt gttgccagtc tggtggtgtt
    21361 cagcaaattt aatgcggtac agcaccggat cgcgcatcac gataaacggt gaagccatgt
    21421 cgattttcgc acgcaggcag gctttacctt cttcaaaacc accggcacgc attttttcga
    21481 acagcgccag gttctcttca acgctgcggt cgcggtacgg gctgttttta cccggttgcg
    21541 tcagggtgcc gcggtattcg cggatctgtt ccggcgtcag ttcatcaacg tacgccaggc
    21601 ctttattgat cagttcgatc gcataggcgt ggagctgatc aaaataatcg gaggagtaac
    21661 ggacgttacc agaccagtga aaacctaacc actctacgtc gtttttgatc gactcaacat
    21721 actcgatatc ttcttttacc gggttagtgt cgtcgaaacg caggttgcac tggcctttat
    21781 agtcctgggc gatcccgaag ttcaggcaga tagatttcgc atggccaata tgcagatagc
    21841 cattcggctc cggcgggaaa cgggtgtgta ctgtggtgtg cttaccactg gccagatctt
    21901 catcgatgat ctgacggata aagttagtcg ggcgggcttc tgcctcactc atcgtggatt
    21961 cctcaaagcg taaacaacgt ataacggcgt atgatcttat aagcgggaca ggctgacaac
    22021 tgttagttag cgaaaatcga aaatctcact gagattatgg ggatgattga tgaaaaaaaa
    22081 aagcggcaga gaagatctcc accgcttttg ccagtcccgc gtcattgttg gatgcgcgtc
    22141 ttatccaacc aggactgagc ttttgtaggc ctgataagac gcgtcaagcg tcgcatcagg
    22201 cataaagcag attacttttt gatttcatac agcggtgttt gacccgccac aacattgccc
    22261 tgagctttaa tgatcaagcc actgaaatcg tcgatattgc tgcaaaccac cgggctaatc
    22321 atcgaacggg cgttagcgtt caggtaatcc agatccattt ccagaatcgg ttgccctgcg
    22381 ctcacctgcg cgccctcttc caccagacgt ttaaagcctt taccttccag cgctacggtg
    22441 tcgataccca tgtggacgac aatctccgcg cctttttcgg tttctaggca gaacgcgtgg
    22501 ttggtgttga agattttcac gattgtccct gcggctggtg atacgacgat tttatctgtc
    22561 ggtttcaccg ccacaccgtc acccaccgct ttgctggcga atgcttcgtc agaaacctga
    22621 tccagtgcca cgacatcacc ggtaatcggc gataccagct ccgcgataga taccgcgttt
    22681 ggtacagcct gcggttttgc tacaggagcg gcagttgccg gagttgcttc agctgacgca
    22741 gctgctaccg ggccacgggc aacgactttc ttcatcgcat cgccaatgga ttctgctttc
    22801 gcgccaacaa tcacctgaat ggtctgtttg ttcagtttta ctacaccaga agcacccaga
    22861 cgtttacaca tcgcatcgtt gacgcgagct gagtcagcca cggtaaggcg cagacgggta
    22921 atacaggcgt caatcgcttt caggttgtca gtaccgccaa ccgcagcaat atagttggtt
    22981 gccagttgag tcagaccttc ttcagtattg ctgttagcct cttcagttac gatctcgtct
    23041 tctttatctt cacgacccgg cgttttcagg ttgaacatgc ggataaccaa actgaacacc
    23101 acgaagtaga tagcgaagaa gacaacaccc atcaccagca gcatccagac gttctggctg
    23161 gcggccggca ggttatacat caacgcgtag tcgatagccc ccgcagagaa agagaagcca
    23221 gcatggatac ccagcagcgt tgccacaaac aggctgatac cggtcagcag tgcgtgcagg
    23281 aggtacagca gcggagcaag gaacatgaac aggaattcca gcggctcagt cacaccggtc
    23341 aggaacgcag taacagcaac agaaagcagc atcccgccaa ccatcggacg acgctctttc
    23401 ggtgctgcga agtacatcgc cagcgccgca cccggcagac cgaacatcat gatcgggaag
    23461 aagccggaca tgaacatccc cgcggtgccg tcaccagcgt agaaacggtt gatgtcgccg
    23521 tggaaaaccg tacccgccgc gttggtgaat tcaccaatct ggaaccaggc gatggtgttc
    23581 agcacctgat gcagaccggt tgggatcagc agacggttga tgaaaccaaa gataccggaa
    23641 cccagcgcgc ctgcagaaac gatccactcg ccgcctgcat ggatagcgtg ctgtactggc
    23701 ggccagacgt aaccaaaaat ggccgccagc accaggcaga aaaagccggt ggcaatcggc
    23761 acaaagcgtt tgccgccgaa gaagctcagg aagtccggca gtttaatatc ggaccaacgg
    23821 ttataggctg cgccaccaac cagaccggta atgatacccg ccagtacacc catgttaatt
    23881 tctgggttga tggtcaccat cgctttggtt aacacaaagt aacctaccgc acccgccagc
    23941 gccgccgcac ctgcgctgtc tttcgaccag ctggatgcca caccgatggc gaagattaat
    24001 gcgaggttat caaaaatcgc accgcccgcc tgggcaataa acgcaacgtt aagtaaatct
    24061 ggctgaccga atcgcagcaa cagtgccgcc accggcagca ccgcgatagg gagctgtaac
    24121 gccctaccga gtcgctggaa aaaacctaaa atattcatct tattccccct acgagaaccc
    24181 tatttggctc gtttaaagcc gtatttttat tttgctgcaa attgtactgc cgatgttctg
    24241 taatcagatt gttagatcat ctgctacaga gtgtgtgaaa atttaattcg tatcgcaaat
    24301 taaacgcgtg ccttttgtga gttttgtcac caaatatcgt tatcatcact cccttttact
    24361 ggctaaagca gaaaacttat tttatcattc aaaaaatcag gtcggattga cgcctgtctg
    24421 cgcaaatcca ggttacgctt aaagatgcct aatccgccaa cggcttacat tttacttatt
    24481 gaggtgaata atgagactga tccccctaac taccgctgaa caggtcggca aatgggctgc
    24541 tcgccatatc gtcaatcgta tcaatgcgtt caaaccgact gccgatcgtc cgtttgttct
    24601 gggcctgccg actggcggca cgccgatgac cacctataaa gcgttagtcg aaatgcataa
    24661 agcaggccag gtcagcttta agcacgttgt caccttcaac atggacgaat atgtcggtct
    24721 gccgaaagag catccggaaa gctactacag ctttatgcac cgtaatttct tcgatcacgt
    24781 tgatattcca gcagaaaaca tcaaccttct caacggcaac gccccggata tcgacgccga
    24841 atgccgccag tatgaagaaa aaatccgttc ttacggaaaa attcatctgt ttatgggcgg
    24901 tgtaggtaac gacggtcata ttgcatttaa cgaaccggcg tcttctctgg cttctcgtac
    24961 tcgtatcaaa accctgactc atgacactcg cgtcgcgaac tctcgtttct ttgataacga
    25021 tgtcaatcag gtgccaaaat atgccctgac tgtcggtgtt ggtacattgc tggatgccga
    25081 agaagtgatg attctggtgc tgggtagcca gaaagcactg gcactgcagg ctgcagttga
    25141 aggttgcgtg aaccacatgt ggaccatcag ctgtctgcaa ctgcatccaa aagcgatcat
    25201 ggtgtgcgat gaaccttcca ccatggagct gaaagttaag actttaagat atttcaatga
    25261 attagaagca gaaaatatca aaggtctgta attgttatcc ctgccctggc tccttgctca
    25321 gggcaatatt ttttaaaatc ggggggcaga atgtatgcat taacccaggg ccggatcttt
    25381 accggccacg aatttcttga tgaccacgcg gttgttatcg ctgatggcct gattaaaagc
    25441 gtctgtccgg tagcggaact gccgccagag atcgaacaac gttcactgaa cggggccatt
    25501 ctctcccccg gttttatcga tgtgcagtta aacggctgcg gcggcgtaca gtttaacgac
    25561 accgctgaag cggtcagcgt ggaaacgctg gaaatcatgc agaaagccaa tgagaaatca
    25621 ggctgtacta actatctgcc gacgcttatc accaccagcg atgagctgat gaaacagggc
    25681 gtgcgcgtta tgcgcgagta cctggcaaga catccgaatc aggcgttagg tctgcatctg
    25741 gaaggtccgt ggctgaatct ggtaaaaaaa ggcacccata atccgaattt tgtgcgtaag
    25801 cctgatgccg cgctggtcga tttcctgtgt gaaaacgccg acgtcattac caaagtgacc
    25861 ctggcaccgg aaatggttcc tgcggaagtc atcagcaaac tggcaaatgc cgggattgtg
    25921 gtttctgccg gtcactccaa cgcgacgttg aaagaagcga aagccggttt ccgcgcgggg
    25981 attacctttg ccacccatct gtacaacgcg atgccgtata ttaccggtcg tgaaccgggc
    26041 ctggcgggcg cgatcctcga cgaagctgac atttattgcg gtattatcgc tgatggcctg
    26101 catgttgatt acgccaacat tcgtaacgct aaacgcctta aaggcgacaa actgtgtctg
    26161 gttaccgacg ccaccgcgcc agcaggtgcc aacattgaac agttcatttt tgcgggtaaa
    26221 acaatatact accgtaacgg actttgtgtg gatgagaacg gtacgttaag cggttcatcc
    26281 ttaaccatga ttgaaggcgt gcgtaatctg gtcgaacatt gcggtatcgc actggatgaa
    26341 gtgctgcgta tggcgacgct ctatccggcg cgtgcgattg gcgttgagaa acgtctcggc
    26401 acactcgccg caggtaaagt agccaacctg acctcattca cacctgattt taaaatcacc
    26461 aagaccatcg ttaacggtaa cgaggtcgta actcaataag agaaagtatg acaccaggcg
    26521 gacaagctca gataggtaat gttgatctcg taaaacagct taacagcgcg gcggtttatc
    26581 gcctgattga ccagtacggg ccaatctcgc ggattcagat tgccgagcaa agccagcttg
    26641 cccccgccag cgtaaccaaa attacgcgtc agcttatcga acgcgggctg atcaaagaag
    26701 ttgatcagca ggcctccacc gggggccgcc gcgctatctc catcgtcacc gaaacccgca
    26761 atttccacgc aatcggcgta cggcttggtc gtcacgacgc caccatcacc ctgtttgatc
    26821 tcagcagcaa agtgctggca gaagaacatt acccgctgcc ggaacgtacc cagcaaacgc
    26881 tggaacatgc cctgttgaat gccattgctc agtttattga tagctaccag cgcaaactgc
    26941 gcgagctgat cgcgatttcg gtgatcctgc cagggcttgt tgacccggac agcggcaaaa
    27001 ttcattacat gccgcatatt caggtagaaa actgggggct ggtagaagct ctggaagaac
    27061 gttttaaagt gacctgtttc gttggtcacg atatccgtag tctggcgctg gcggagcact
    27121 acttcggtgc aagtcaggat tgcgaagact ccattctggt gcgtgtccat cgcggaaccg
    27181 gggccgggat tatctctaac gggcgcattt ttattggccg caacggtaac gtcggtgaaa
    27241 ttggccatat tcaggtcgaa ccgctgggtg aacgctgcca ctgcggcaac tttggctgcc
    27301 tggaaactat cgccgccaac gctgccattg aacaacgggt gttgaatctg ttaaagcagg
    27361 gctaccagag ccgcgtgccg ctggacgact gcaccatcaa aactatctgc aaagccgcga
    27421 acaaaggcga tagtctggcg tcggaagtaa ttgagtatgt cggtcgtcat ctgggtaaaa
    27481 ccatcgccat tgctatcaac ttatttaatc cgcaaaaaat tgttattgcc ggtgaaatca
    27541 ccgaagccga taaagtgctg ctccctgcta ttgaaagctg cattaatacc caggcgctga
    27601 aggcgtttcg tactaatctg ccggtggtac gttctgagct ggatcaccgc tcggcaatcg
    27661 gcgcttttgc gctggtaaaa cgcgccatgc tcaacggtat tttgctccag catttgctgg
    27721 aaaattaatg tgcttttata gtggcgctta ttgttgtcaa tattctgggt agtccatgac
    27781 cattaaaaat gtaatttgcg atatcgacgg cgtgctgatg cacgataacg tcgccgtacc
    27841 gggtgcagcg gaatttttgc acgggattat ggataaaggc ctgccgctgg tgttgctgac
    27901 caactatcct tcgcagaccg ggcaagatct ggcgaaccgc tttgccactg ccggtgtcga
    27961 tgtgccggat agcgtgtttt atacctctgc gatggcgacc gccgatttcc tgcgtcgcca
    28021 ggaaggcaag aaagcatatg tggtgggcga aggcgcactg attcatgaac tgtacaaagc
    28081 cggtttcact attaccgatg tgaaccctga tttcgtgatt gttggcgaaa cgcgttccta
    28141 caactgggac atgatgcata aagcggccta tttcgtcgct aacggtgcac gctttatcgc
    28201 caccaacccg gacacccacg ggcgcggttt ttatcccgct tgtggcgcgt tgtgtgcagg
    28261 gattgagaaa atctccgggc gcaaaccgtt ctatgttggt aagcccagcc cgtggatcat
    28321 ccgcgcagca ttaaacaaaa tgcaggcgca ttcggaagaa acggtgattg tcggcgataa
    28381 cttgcgcacc gacattctgg cgggctt
//
LOCUS       DAJVQG010000039        27627 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJVQG010000039 DAJVQG010000000
VERSION     DAJVQG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.039"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..155
                     /locus_tag="OQM83_001527"
     CDS             <1..155
                     /locus_tag="OQM83_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1519817.1"
                     /translation="GRLLRRDRPDIAVRYYYKGVLWSPGYFANSCGGAPISVIRQYIE
                     QQQTPG"
     gene            complement(286..1609)
                     /locus_tag="OQM83_001528"
                     /pseudo
     CDS             complement(286..1609)
                     /locus_tag="OQM83_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1609..3225)
                     /locus_tag="OQM83_001529"
     CDS             complement(1609..3225)
                     /locus_tag="OQM83_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCS1519818.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3521..4237
                     /locus_tag="OQM83_001530"
     CDS             3521..4237
                     /locus_tag="OQM83_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1519819.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4234..5895)
                     /locus_tag="OQM83_001531"
     CDS             complement(4234..5895)
                     /locus_tag="OQM83_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCS1519820.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6091..6519)
                     /gene="ibpB"
                     /locus_tag="OQM83_001532"
     CDS             complement(6091..6519)
                     /gene="ibpB"
                     /locus_tag="OQM83_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCS1519821.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6631..7044)
                     /gene="ibpA"
                     /locus_tag="OQM83_001533"
     CDS             complement(6631..7044)
                     /gene="ibpA"
                     /locus_tag="OQM83_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCS1519822.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7038..7110)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7350..7682
                     /locus_tag="OQM83_001534"
     CDS             7350..7682
                     /locus_tag="OQM83_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCS1519823.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7684..8898)
                     /locus_tag="OQM83_001535"
     CDS             complement(7684..8898)
                     /locus_tag="OQM83_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCS1519824.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            8999..10063
                     /gene="cbrA"
                     /locus_tag="OQM83_001536"
     CDS             8999..10063
                     /gene="cbrA"
                     /locus_tag="OQM83_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCS1519825.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10060..11397)
                     /locus_tag="OQM83_001537"
     CDS             complement(10060..11397)
                     /locus_tag="OQM83_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1519826.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11472..12620)
                     /gene="dgoD"
                     /locus_tag="OQM83_001538"
                     /pseudo
     CDS             complement(11472..12620)
                     /gene="dgoD"
                     /locus_tag="OQM83_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(12617..13234)
                     /gene="dgoA"
                     /locus_tag="OQM83_001539"
     CDS             complement(12617..13234)
                     /gene="dgoA"
                     /locus_tag="OQM83_001539"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCS1519827.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13218..14096)
                     /gene="dgoK"
                     /locus_tag="OQM83_001540"
     CDS             complement(13218..14096)
                     /gene="dgoK"
                     /locus_tag="OQM83_001540"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCS1519828.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPAHFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14093..14782)
                     /gene="dgoR"
                     /locus_tag="OQM83_001541"
     CDS             complement(14093..14782)
                     /gene="dgoR"
                     /locus_tag="OQM83_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCS1519829.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            14751..15383
                     /locus_tag="OQM83_001542"
                     /pseudo
     CDS             14751..15383
                     /locus_tag="OQM83_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15429..16241)
                     /gene="yidA"
                     /locus_tag="OQM83_001543"
     CDS             complement(15429..16241)
                     /gene="yidA"
                     /locus_tag="OQM83_001543"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCS1519830.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16356..16754)
                     /locus_tag="OQM83_001544"
     CDS             complement(16356..16754)
                     /locus_tag="OQM83_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCS1519831.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(16994..19408)
                     /gene="gyrB"
                     /locus_tag="OQM83_001545"
     CDS             complement(16994..19408)
                     /gene="gyrB"
                     /locus_tag="OQM83_001545"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCS1519832.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19437..20510)
                     /gene="recF"
                     /locus_tag="OQM83_001546"
     CDS             complement(19437..20510)
                     /gene="recF"
                     /locus_tag="OQM83_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCS1519833.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20510..21610)
                     /gene="dnaN"
                     /locus_tag="OQM83_001547"
     CDS             complement(20510..21610)
                     /gene="dnaN"
                     /locus_tag="OQM83_001547"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCS1519834.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(21615..23018)
                     /gene="dnaA"
                     /locus_tag="OQM83_001548"
     CDS             complement(21615..23018)
                     /gene="dnaA"
                     /locus_tag="OQM83_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCS1519835.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23315..23395
                     /locus_tag="OQM83_001549"
     CDS             23315..23395
                     /locus_tag="OQM83_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1519836.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            23625..23765
                     /gene="rpmH"
                     /locus_tag="OQM83_001550"
     CDS             23625..23765
                     /gene="rpmH"
                     /locus_tag="OQM83_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCS1519837.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            23782..24141
                     /gene="rnpA"
                     /locus_tag="OQM83_001551"
     CDS             23782..24141
                     /gene="rnpA"
                     /locus_tag="OQM83_001551"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCS1519838.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24105..24362
                     /gene="yidD"
                     /locus_tag="OQM83_001552"
     CDS             24105..24362
                     /gene="yidD"
                     /locus_tag="OQM83_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCS1519839.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24365..26011
                     /gene="yidC"
                     /locus_tag="OQM83_001553"
     CDS             24365..26011
                     /gene="yidC"
                     /locus_tag="OQM83_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCS1519840.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26117..27481
                     /gene="mnmE"
                     /locus_tag="OQM83_001554"
     CDS             26117..27481
                     /gene="mnmE"
                     /locus_tag="OQM83_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCS1519841.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6636 a         7486 c         7059 g         6446 t
ORIGIN      
        1 cgggtaggtt actgcgacga gatcgaccag atattgcagt caggtattac tacaaaggcg
       61 ttttgtggag tcctggctat tttgccaata gctgcggagg tgcgccaata tccgtcatcc
      121 gccaatacat tgaacaacag caaacacctg gttaggtgga aaaccgcgcc ttatatcccc
      181 gccctgaagg acggggtttt acggcgcacc ggataaattt ggttatgcgc cgtcagccgt
      241 gctaaagaat acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg
      301 ccagtacgct ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct
      361 cggtaccgtt ttcgataacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca
      421 ggcgtccacg accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg
      481 cgggatatcg ccgaccacca gtggttctgg tccgttgtgc ccgaccagac acgggatctc
      541 gaccatcgct tcatcatcaa agttatggat tgccccgttg ttaggcacaa tcagcaacat
      601 ccgctcctga gtgttgaagg caattgccgc cgccagatcg acgatgtatg acgcatgttc
      661 gtcaatctcc agcttggccg ccgctgaatt tcctgccgct gtaatggcgc ggcaagcatc
      721 gaaaacctgt ttctcgcgat gttccattca cctcattcgc gcgggtatgt tcagggttgg
      781 aatgctgaac aacataatcc gggaagagat aatatttcag ataggtgttc ggcaatgtat
      841 ccgggtccag tgcctggaca tcccgcgctt ttgcataggt gtcgttccag ctcgcttcag
      901 tatgtgtatc ttgctgcggt ggaacataac cgtgcttcga gacatattgc cgcagctggg
      961 gcatcaggtc gttgccctgc aaatcttcaa ttgatgtcca ccagccaaag tggttcaggc
     1021 cgtagtagcg cacgcgcatc tgtttgcgat cttgcagccc aacaatttgc gccatccggc
     1081 tttcaatacc gattggcatg tcacagatgt tgaggatttt cgcattcggg cgcagacgac
     1141 gcgtggcttc tgcgacaatg gctgccgggt tggagtagtt gagcatccag gcatttggtg
     1201 aatatttttc catataatcc accagttcca ggacgccgcc aatggaacgc atgccgtacg
     1261 ctattccgcc gggtccgcaa gtttcctgac caacaacacc gtggcgcagt gggatttttt
     1321 catccagttc gcgcatcggg tacttgccga cgcggatgtg cgccatgaca aaatcaacgt
     1381 cgctgaacgc cacttcagga tcggtggtgt aactaaacgc aatgtccggc gctttctctt
     1441 taaggatgac tttacaggct tcggcaatca cttcctgccg cgcgccatcg ttatcataaa
     1501 atttcagtgc acgaagcggg aaacgctcct gattcgccag gagcatcaac acgatgcctg
     1561 gcgtaaaggt gcttccaccg cctgcgacaa ccactgagaa tttggtcatt atactgcctc
     1621 cgtaatggca acattttctg ctgattgatg agaattaatt aagctatcga gctgttcacg
     1681 cagctgggat acatgcagac caatgatcac ctgaatggca tcgccactac ggaagacgcc
     1741 gtgcgctccc agctttttaa agacttcgtc atccagcgtt tgtgacatgt catgcagtgc
     1801 aatgcgtaga cgcgtcgcac aattgttaat gctggagata ttgccgaccc cgcccagggc
     1861 ttgcaggata ccggcagcct gatccagctc tttttttggc tctgccgcgg tggtttggcc
     1921 tcgcgaggct ttgtattcgg cttttgagta gagtttcact tccgcatctt cacgtcccgg
     1981 cgtgcacatg ttgaactgca gaatcagggt gcggaaaacc acgaagtaca gcagggtaaa
     2041 gcacaaccca atggcgattt gggtcagcat catatccgcg tggttgctga acatcgggat
     2101 ccagttttgc ggtaaaacct ggtcaatcag acctccgccc atgttgccca ccacaccaaa
     2161 gagatacatc acggtcgaca ttgaggccgc aagcacagcg tgtaccgcaa acagcaacgg
     2221 tgaaatgaac aggaaggtaa attccagcgg ttcggtaatt cccaccagca tggcggttaa
     2281 ggttgcggga atcagcaagc ccgccacttt tacccgattt tccggtgcgg cagtgaagta
     2341 catcgctaaa gaaatgccca cggcaccaaa gatttttgag ttaccgtgca gggcaaaacc
     2401 gccttccggg aacaacgatt tcagcggctc ggcgctcaaa ctgaactctt gcagatgctg
     2461 cgcccagtac atctgaatgc cgccttcaac agctgccgga cccaagataa acggtccgta
     2521 gatgaagtgg tgtaaaccgg ttgggatcag aatacgttcg aggaaggtgt aaacccagac
     2581 cccaagcgca cccgccgaac gcaggaacgc ttgcagagat tcaatccccg tttgtacttt
     2641 tggccagccg agcaatgtca gccaggcaca ggggatcatc accaggaagg cgataatcac
     2701 cacataagac gtcccctgga aaatgccgag aaaaaccggc agttttttat cgaacagacg
     2761 gttatgcagc gccgtcacaa tgccggaaat gataattgcg ccgataatgc tggtatcgag
     2821 ggttttaatc ccggccatca ttgtcagacc gctacctgcc accgcgtcct gagtgaaatc
     2881 gacgccgaag tagcttcccc aggtcattcc catcgcgttg atgaaatagt tccaggtcag
     2941 gaaactcacc atcaccgcca gacaagcacg tccctgcgct tgcttagcaa ggccaatggg
     3001 taaaccgaca gcaaaaatca gcggcatatt acggaatacc gtccaaccgc cctcttcaat
     3061 aatgtgtacg atttgcgcga ataaactgtt cggatcggtc agtgattccc cgacaaacat
     3121 cgggttttgc agcaagatgg caagacccac cacaatcccg gcgaagggaa acagcagcac
     3181 tggcgtaaac atcgcgccgc caaagcgttg aatttgactg agcatttcca catcctcttt
     3241 tctcaattct gtaagtagga agcctgattg gttatgcggc aggcctggag caaggatagg
     3301 aagatgcgat cggcagaaca tgacgaccca atgggattcg tgatcgcttt catgcttttc
     3361 gttgggcggt agagatctac gttttcgcga aaaagtagac atctgtgagc acaggtacac
     3421 agcatcgaac acatctttaa aaaaaagatg ttttttcaat cgattaagca gaacttgtgt
     3481 gcgcattacc cgggcttgca ggcaaaaaag agatctagag atgatctaca aaagcattgc
     3541 ggagcggtta agaattcgac ttaactccgc agatttcacg ctaaacagcc ttcttccggg
     3601 tgaaaaaaag ctggcggaag agtttgcggt atcgcggatg accatccgta aagccattga
     3661 cctgctggta gcgtgggggc tggtggtccg ccgccacggt agtggcactt acctggtgcg
     3721 caaagatgta ctgcatcaaa ccgccagtct gaccgggctg gtggaggtgt taaaacggca
     3781 gggaaaaact gtcaccagcc aggtgctgat ttttgaaatc atgcctgcgc ctccggccat
     3841 tgccagccag ttacggattc aaatcaacga gcagatctac ttctcccgtc gcgttcgttt
     3901 tgtggaaggg aaaccgctga tgctggaaga cagctatatg ccggtaaaac tgttccgtaa
     3961 tctttcgctg caacatctgg aagggtcgaa gtttgaatat attgaacaag agtgcgggat
     4021 tttgattggc ggtaattatg aaagcctgac gccggtgctc gccgatagac tgctggcgcg
     4081 gcaaatgaag gtagcggaac acacgccact gctgcggatc acctcgttgt catatagcga
     4141 gagcggggag tttttgaatt attcagtgat gttcagaaat gccagcgaat accaggtgga
     4201 gtaccattta cggcgactcc acccggaaaa gagttaaccg atactccaga agagcaccgc
     4261 cagtaattgg ggggtgataa tgcgcaggaa catcactaac ggatagacag tggcgtaaga
     4321 gagcgccgcc gcaccgctag ttggatgaag attattagca aacgccagcg ccggaggatc
     4381 ggtcatggaa cctgccagca tcccgcacat ggtcaggtaa ttcattttgg ctaacatccg
     4441 cgccagaatg ccaacagtaa tcagcggaac ggcggtgatc agggcaccat aaccaatcca
     4501 gcttagccct tcgccattga ccagggtatt cacaaaatcc ccaccagatt tcagaccaac
     4561 gaccgagagg aacagcacga tccccagctc ccgcagcgcg aggttggcac ttggcggcat
     4621 aaaccagtac agcttgccga tactgccgat acgcccgagg atcaacgcca taatcagcag
     4681 accgcccgcc agccccagtt tcaacgcggc cgggaatccc ggcacaaaga cgggaataga
     4741 acctaacaat acgccaagcc cgatgccaat aaacactggc agcatctgaa cctgttgcag
     4801 tttttgctgc gcattcccca gcacattggc aacggcatca attgcggacg gacgccccac
     4861 caggttgagg atatcgccga actgcaggct gatatcgcca ctggcgacca gttcgacccc
     4921 ggcacggttc aggcgcgaga taacaacgtc atagcgttct ttaaagtgca ggtcgcgaat
     4981 acgttttccg agcacgtttt cattggtgac caccacacgc tcgacgcgca aatcagtgtc
     5041 tttcgtggac agcgaagtat cgacctcctg accaatcacc agttgcgcat tatgtaaatc
     5101 cgctggctga cccaccagat gcagcaaatc gcccagttgg ataatggtat ctggcgaagg
     5161 aacttttagg gtttcttcgc gtttcagacg cgagcagata attttgtcgc cgttgagaat
     5221 cggtacatct ttaatggcta aatcatgcag gttagggttc tcaacgcgaa tattgatagt
     5281 cttgatcagc gcgccgccat tggtgcgtga agactcgtgc tgctgagctt ctgtctcgac
     5341 attgacgcgg aaaataaccc gcaacatcca catggtgaac aaaatcccgc aaatgccgaa
     5401 tggatacgcc atcgcgtaac tcatccccat ctgatcgacc atttccattg gtgtacccag
     5461 gtcgcgcaaa atctgctgcc ctgcccccag cgctggcgta ttggtaaccg caccggagaa
     5521 aatccccagc actaccggca gtggaatatc aaacagttta tgcaggatgg cggtaaccag
     5581 accaccgatg atgacgatca gaacagcaaa caggttgagg cgtaatccgg agacgcgcaa
     5641 tgaggcaaag aagcccggcc ctacctgaat cccgatggta taaacgaaca ggatcaggcc
     5701 aaattcctga ataacatgca gcatatcgct acttaatgtc atccccgcct gagaaacaaa
     5761 atggccgacg atgatcccac caaacagcac gccgccaata cctaatccta tgccgcgaaa
     5821 tttgacgttg ccgataaaca aaccgacgac tgccaccaaa gccagaatac tgaccgttaa
     5881 tgctatatca ctcatcgtcc atttctcttg agataacatt ttaattgcta cggattatgg
     5941 ctgaatcggt gaagcagatt tgccttatag cgcacattat tacgggaaag aacgtgccga
     6001 aatatcttaa acagtcgaaa tttaacgcat tgttgaagaa gaaagacaaa aaaagcccca
     6061 ccagaatggc ggggcaaaga gaatagctag ttagctattt aacgcgggac gttcgctgat
     6121 agcgatacgc tgcgctgcga tgggttcagg ctcattacga attaaatcaa tatgcagtaa
     6181 accgtttacg aaagttgcgc cagagacttc catattttca gccagcgtaa agctcaggct
     6241 aaatggctga ttcataagcc cttgatgcag ccattttttc tcttcttttg gctgctccgg
     6301 cgtgcctttt acgctcaggc gcgtaccttc cagttgaatc tctaaatctt cctgacggaa
     6361 acctgccagc gcaagggtaa tgcggtagtg gttatcgtcg cttttctcaa tgttgtacgg
     6421 cgggaagctc tgactttcac cggcgttttg cagtgcgttg gccagtttgt caaaaccgat
     6481 ccattgacgc atcagtgggg ataaatcgaa gttacgcata gtcatttctc cttctaagaa
     6541 gcgagtaagt acctgcaaat ccgaagattc gcatatgctc cctgacggcg agcatggaga
     6601 tgtcaggccg cgccaggcgg ccttaaggaa ttagttgatt tcgatacggc gcggtttttt
     6661 cgcttccgga atcacgcgtt cgagatcgat atacagcaaa ccatttacca ggttagcgcc
     6721 acgaacatga atgttctcag ctaactggaa tttgcgttca aagttgcgtt cagcgatgcc
     6781 ctggtacaga taggtgcgct ctttttgttc gtcggcgtga gcacctttca ccaccagcag
     6841 attatcctgg gcggtaattt ccagttcgct ctcagcaaaa ccagccacag cgatagcaat
     6901 gcggtaatgg ttttcgtcta ccagttcaac gttatacgga gggtagccgc cattactctg
     6961 gctctggttg ttttctaagt ggttaaacaa acggtcaaat ccaatagcag aacggtaaag
     7021 cggggataaa tcaaagttac gcataatcaa tagctcctga aatcagcgag aatgtaagac
     7081 cttccacaat ggacaggtca ggtagccaga acacccatca ggcgcgtcct catcgactac
     7141 gatgtaaaaa tgggtctgga aatgaacttt tcaagcctta tcagcggact tttttttgca
     7201 gtttatggtc tattgcatag actgaggggg cagcacgaaa cgttaaagtg tgatagccgc
     7261 cacagagcga cgtaatgatg atgttatttt cagcaaggga tcgctataac tcatcaggcc
     7321 aacacccacg atcaacagat gaatttatta tgataagaaa tgtgttgtta gcgttcatga
     7381 tatgcagcgg aatggcatta ctcggaggat gctccagcgt gatgtcccat accggaggga
     7441 aagaaggaac gtatcccggc acccgcgcca gcgccaccat gattggcgat gatgaaacca
     7501 actggggcac taagtcgctg gcgattctcg atatgccgtt taccgctgtg atggacacgc
     7561 ttttattacc gtgggatgtg ttccgcaaag acagctcggt gcgctcacgc gttgaaaaaa
     7621 gcgaagccaa cgcgcaagcc accaatgcgg ttattccacc ggccagaatg cctgataact
     7681 gatttagcga tcagtttcgg tgatccacag ctggccacct tccatccacg ccagccattg
     7741 accatccggc gagaagacca cggcgtccgc agaaggtgga ttggcgtggt tttcggttaa
     7801 atactcaacc tcgccgcttt gcgcatgggc acaagcaatt cgattatcca gcacaaagcc
     7861 caaccattct cctgacggat gccagttaaa tgcagactga atatccgttt tgttatgggt
     7921 taactggcgc ggctcgccgc cctgtggcga gataagccac agttgcacaa tgccgttatc
     7981 atcacgcatt aaaaacgcga tttgcgtacc ctgcggatta cagcgcaccc agtggcgcgg
     8041 gacgttgact aaccccggat aagcccgatg gtgggtaaag gttaaacgtc gctgcacgac
     8101 gccacgcggt ggcgcgggca gggtggtttc cgttccactt aacggcgcat cacctgccgc
     8161 tttccagcca gcttcatctt gcggcaactc aacgataaac agctccggca ctttctcacc
     8221 ctttggcgaa agtgtgtcgc caataaacgc cagcgcgtga tttcctaccc atccttcttc
     8281 ataagcacga ttgatttcat cactgccagg ctgcggcgtg ggcgtggttt tactcaccag
     8341 cacacaccag tggctgccgc tgtattcacg cggatgctgc ttttgtacgt tgaccggacc
     8401 aaacggcgca gcaacaccga cgtttcgcaa atccagcgcc ggatcgagtt catgcattac
     8461 atggtcgtta taggtaaagc tcaccctttc accgttcggg ctaaagacat gcacatggct
     8521 gccgccgcgc agcgctcctg gcgtatacgg cgcggtaata tccattgcat cgagattgct
     8581 caccttgccg ccttcagcaa tgactccgcg ccgatgatgg aagtcgtaat gccatgtttc
     8641 atcaggattt tccgggccgt gaataaagac atatttctct gacttcggat gaacggtcac
     8701 cacgccgacg tgtgcgccct gtgacgcaca gtagataacc tcgacctcac cggtatggat
     8761 attcacacgc tcaatggtct cgccggtaaa cgacgcccca gaaggacgca cgtcaaatac
     8821 cagccactgg ctgtcgggcg tccaggtatt ggtattggtg agcaggtgat tacggggagc
     8881 aaaggttatc tgtttcatgg cgcggtcctg atacaaaacg ggtccagcca tcatacttca
     8941 caaggacttc actttcagac aatagtcttt tgccatcgtg atgaatgtga gtgaaaagat
     9001 ggaacatttc gacgtggcga ttattggcct cggcccggca gggtcggcgt tggcacgaaa
     9061 gttagccggc aaaatgcagg tgatcgcgct ggataaaaag caccagcatg gtactgaagg
     9121 tttcagcaaa ccttgtggcg gtctgctggc accggacgcg cagcgatctt ttattcgcga
     9181 tgggctgacg cttcctgtcg atgtgatcgc taatccgcag attttcagcg tcaaaaccgt
     9241 cgacgtcgcc gcatcgctca cgcgtaacta ccagcgaagc tatatcaata ttaatcgcca
     9301 tgctttcgac ttgtggatga aatcgctgat ccccgccagc gttgaggttt atcacgacag
     9361 cctgtgtcga aaaatctggc gtgaggatga taaatggcat gtcatttttc gtgcagacgg
     9421 ctgggagcag catattaccg cccgctatct ggtcggtgcc gatggtgcca actcgatggt
     9481 gcggcgacat ctctacccgg atcatcaaat ccgtaaatat gtcgcaatcc agcagtggtt
     9541 cgcagagaaa catccggtgc cgttctactc ctgcatcttt gataatgcga taactgactg
     9601 ttattcatgg agtatcagca aagacggtta ttttatcttt ggcggtgcct atccaatgaa
     9661 agacggtcag acgcgtttca cgactctgaa agagaaaatg agcgcctttc agttccagtt
     9721 tggcaaagcg gtgaaaagcg aaaaatgcac ggtgctgttt ccctcacgct ggcaggattt
     9781 tgtctgcggt aaggacaacg cctttctgat tggtgaagcg gcgggattta tcagcgccag
     9841 ctcgctggag gggattagct atgcgctgga tagcgcagac attctgcgtt cggtgttact
     9901 gaagcagcca gagaagctca ataccgctta ctggcgcgcc acccgcaaac tgcgtttaaa
     9961 actcttcggc aagatagtaa aaagccgatg cctgaccgca ccggctttaa gaaagtggat
    10021 tatgcgcagt ggtgtggcac atattccaca gttgaaggat tagccaacgc gcttcacatc
    10081 gcccaccagc aggatataag agagcgcgcc aatcaacgcg acggcggaga tataaaccag
    10141 tgcaggtgcg aaaccgtaac cctgcgccag gtaccccacc accagcggaa cggtgatgcc
    10201 gcccagacca ccggcgaagt taaacacgcc gccggttaaa ccaatcaggc gcatcggtgc
    10261 cagagaagaa accagcgacc aggtaattga agcaaaaccg ttaccgaaga atgccagcgc
    10321 catcaggcac ataatcatca tcggatcgtt agtgtagtta gcgcccataa tgcaggtgga
    10381 gatcagcaag ccgcagataa tcggcgtttt acgcgcaaag cccagtgaaa agcccttacg
    10441 taccagcaga tccgctaccc agccagagag caggacgccg acaaacgccg cgaggaatgg
    10501 caccgtggtc ataaagcctg ctttcagcgc cgtgattcct ttttcctggg ttaaatagtt
    10561 cgggaaccag gttaagaaaa accacagtgt agaagccacc gcaaattgcc caagatagac
    10621 gccgatcagt ttacgatgga acaccagttt ccagtctttg gctgttaacg gctgacgtgc
    10681 ctctttcttc actggcgcat caccatccac cagaccgccg ccatcacgaa tgtaatccag
    10741 ttcagctttg ctgatgcctt tggtcaggcg cggcggctga taaaccttaa accaaatcag
    10801 cgaccagata atgccgatac caccagtcac aatgaacacc cagtgccagc tcaacatctc
    10861 ctgaatccag atcagcagcg gagtcaaaaa cgccagaccg acaaactgac cagacgtata
    10921 aaaaccaacg gcggaggcgc gttcatgttc cgggaaccag ctggtcacca tccggttatt
    10981 ggtcgggaaa gcgggcgctt cgaaaatacc ggttatcgca cgcagaccaa ttaatgacat
    11041 taatcccgtg gcaaagccct ggaataaagt cgccactgac cagccaaata tcgcaataaa
    11101 ataagtcacg cgagaaccta cgcgatctaa aaaccaaccg ccggggatct ggcatagcgt
    11161 ataaagccag gcgaaggccg aaaatacata gcccatttcc gctttcgtaa tgccgaactc
    11221 ttcctgaata tgggcggaag ccacggccag gttagcgcgg tcaacataac aaatgaccac
    11281 cgtaataaag atcatcacca gcgtcagata acgccgacgc cccggctttg ctgcattaac
    11341 gggaatatcc atagcgagct ttctccagat tttgggcata gcgaagccgc tcaccatgcc
    11401 ctgtaattta cagagggtta tatttttgta ttgctgtttt agtgcccgat gaggggctta
    11461 cgtggcagga attaccactc tgctacgctg ttatcttcat gacgccagag tggattacgc
    11521 cagtccgggg catttttact gaactcaata actttagctt cgtcgatttc cacgcctaag
    11581 cccggtttcg ttagcggttt aaagaagccg ccaaccatgc tgaagtcttc tttgtttttc
    11641 acaaagtcga gtaactccgc gcctttgttg taatgaattc ccatactttg ttcctgaagt
    11701 accgcgttat aggaaacaaa gtcgatatgc agacaagccg ccagtgcaat cggtccgagc
    11761 ggacagtgcg gcgcaagggt cacgtcatag gcttctgcca ttccggcaat tttgtagcat
    11821 tcggtaatac cgcctgcatg ggagagatcc ggttgtagaa tcgaaatacc gcctgcctcc
    11881 agcacgcgtt taaaatcgaa acgtgagaac atgcgctcac ccgccgccag tggaatatgc
    11941 gtttgtgccg ccaatttcgg gtagtattcg gcttgttccg ccagcaccgg ctcttcaata
    12001 aacagcgggc gatacggctc cagttcttta atcagcactt tcgccattgg cgcgctgacg
    12061 cgaccgtgga aatcaagacc aaactcaatc tgattgccaa aagcttcacg aatttgtgcc
    12121 acggtgttga ctgccgcatc taccgcgcgg gagttatcaa ttagccccag ttcttcacaa
    12181 ccgttcagtt tgaaggtatc gaagccgatt tcgcgcaggg ttttaatacc gtcgatcaca
    12241 tccgccggac gatcgccgcc gacccaactg taggctttaa ttttgtcgcg aaccaggccg
    12301 cccatcagtt gccagaccga cgcattcagc actttacctt tgatatccca taacgcctgg
    12361 tcgatcccgg caatggcgct catcaggatt ggaccgccac gataaaatcc ggcgcgatac
    12421 atcacttgcc ataagtcatt gatgcgctat ggatcctgac caatcaaata gtcacccagc
    12481 tcgtgaactg ccgcttccac cgtgcgggcg cggccttcaa tcactggctc gccccagccg
    12541 acgacgcctt catcggtttc aattttcagg aacatccagc ggggaggtaa acgatacgtg
    12601 gtaattttgg tgattttcat tgcactgcct ctcgatacgc cttaacaaat gctgctgcct
    12661 gctgcgcggt gcgttctacg gactgcccgg cgcgatagag atcgctgcct aagcccgccc
    12721 cagcacaacc tgcgtctatc cactgcgcca ggttttctgg cgtcacgccg ccaacggcaa
    12781 agactgcgat gtccgatggc aataccgctt ttaacgcttt gatgtattgc ggaccaaaag
    12841 ccgatgacgg aaatattttc agcgcctgcg cgcccgcttc gagcgcggta aaggcttcgg
    12901 tcgccgtcgc gcagccgggg cagacggtca tgccgtagcc caccgcacgg cggatcactt
    12961 cactatggat attgggcgta acgatgagct gacagcccat cctggcgagc gcatcgacct
    13021 gttcaggttt cagtaccgta cctgcgccaa tcaacgcctt gtcgccatac gcatcaacga
    13081 tggcgggaat gctttgctcc cattgtgggg aattcagcgg gatttcaacc gcgtcgaacc
    13141 cggcgtcaat caccgcgcca acatgcgcca gcgcctcgtc gggcttaatg ccgcgcaaaa
    13201 tggcgatcag cggaagttta gtttgccact gcatgagcga tgctccttat accagcctga
    13261 aatgccgtgt cgcccgccac cgccgtcacg tcgcaaccca tcgcctgaaa ggcttgctgg
    13321 tagcgcgcgg tcaacgatgt tccggcgaca agggtgatgg cgtgttgatg ggccacatag
    13381 tcgcgcatac tggcgacctc tgcgccaatc aacaaaccag agagaaattc actgacctgt
    13441 tcacggggaa gtgttcccag cacatgcgag gcacgaactt caaaaagctg cggcaatatg
    13501 gcgggcgtat taagaccacg ctcaaggcca gccgtgaagg catcggcaga gttttcctgc
    13561 ggcggcaaac ctgcaccaat caatgagtga tttaacagta aatgatgtaa ttcaccggtc
    13621 atcacggtgc gaaaatcgtt gatttgctgg ctatcggcct gcacccattt gcaatgcgta
    13681 ccgggcatga cataaagaga ggaaggagcc agagctcgcg cgccgatcaa ttgagtttct
    13741 tcgccgcgca tcacattgtg gttatcatca tgagagacgc ataatccggg aataatccag
    13801 atattgtcgc caacagacgt taattgttcg ccaatagacg aaaaatgggc aggaacagat
    13861 aaatacggag caactttcca gccgacattg ctgccgacca ttcctgccat taccactggc
    13921 gtattctctt cacgccagtc ggtcgtgact tctgctaaca ccgcagccgg agattttccg
    13981 ttcaggcgcg tgacgcctgc ttctgattgc ctgctctcca ggcagtggtc gccctgataa
    14041 agccaggcgc gcagattggt cgatccccag tcaattgcga tgtagcgagc tgtcatgtga
    14101 tttcctttaa ccttcgtgtc gagctggcga tcatggtaag cgccgcctgc tctgccgcat
    14161 cgccgtcctg atgccgtatc gcatcgaaca gcgccttatg ttcctggagc gtttgcggca
    14221 tgttggcctc atcgcccatc caggttcgtt caaaaaccgc ccgctgcagc gaactgatcg
    14281 caatgctaag ttgctgtaac accgggttat gcaccgactg cagcaccgcc tcgtggtagc
    14341 gaatatccgc ttcgttaaac gcttcgcggt cctgattgtt ggcaatcatc tcgttcagcg
    14401 ccgattcaat ctgcgccaga tcgctggagg tcgcgcgctc tgctgcccaa cgggcaatcg
    14461 ccggttccac cagatttcgc acttcgctca tggcactgat aagccgtggg tcgtagtcgt
    14521 tttccagcac ccattgcagt acgtcagtgt cgaggtaatt ccactggtta cgcggtgcca
    14581 caaacgcgcc gcgataacgt ttcatttcaa tcagccgttt cgccatcaac gaacggaaca
    14641 cctcacggat gatgttgcgc gaggttgcaa actcctcaca gagttccgcc tcagccggaa
    14701 gcggcgagcc tggtacgtat ttgccgtgaa cgatctgttt acccagcgta atgacgatgc
    14761 gatcggtttt attgagagtc atggagagtc cttgtgctct gtatgttctt ctctacttta
    14821 ccccgatcga tgcataatca ggaaagtatc aatcgcagtc agacaccatc acggggctat
    14881 cgcaggcaaa ggatagtaat ggaacaaaag gctatgtttt tgtaggggaa tcgctggatt
    14941 accttatcac agatggtgcc gatgacatcg ttaagatgct caaggatcca gctcttaacc
    15001 ggcacaatat tcaggttgct gatgacgcaa gatttgtttt aaatgcgggg aaaaagaaat
    15061 ttaccggcac aatatcactc tattacaact ggagcacaga agaagaaaaa gcacgagtga
    15121 cacaatatcg ttttgcttgt ggtgttcagc actgtacccg gacagtagaa aacctggaag
    15181 gtactatcca tgagaaaaat aaaaacatgg attactcaaa cgtaatggct ttctaccatc
    15241 catttaaagt gcgattttat gaataccatt cacccagagg cattccggat ggtgtttccg
    15301 cagcattact gccagtgact gttacgctgg acatcattac tgcaccgctg caatttctgg
    15361 ttgtatatgc agtaaaccaa taatcagtaa gcgggcaaac gcgtttatgc tgtttgcccg
    15421 cccacagatt aattcagcac atacttctca atagcaaacg ccacgccatc ttcaaggtta
    15481 gatttggtga caaagttcgc cacttctttc accgaaggaa tagcgttatc catcgccaca
    15541 ccgacgcctg catattcaat cattgcgatg tcgttttcct gatcgccaat cgccatgatt
    15601 tcttccggtt taatacctaa cacgtcggcc agtgatttca cacccgtacc tttgttaacg
    15661 cgtttatcga ggatttcgag gaagtacggc gcacttttca gcacggtata tttctctttc
    15721 acttcctgcg gaatacgcgc gatagcctgg tcgaggatgg cgggttcatc aatcatcatc
    15781 actttcagga actgggtatt ggggtccatt ttctccgctt cgcagaacac cagcggaata
    15841 gtggcaacga aagattcatg cactgtgtag tagctgatat cgcggttggc ggtgtacagc
    15901 gtggtgcggt ccagggcgtg gaaatgagaa ccgacttcgc gagagagttt ttccaggaaa
    15961 cgatagtcgt catagctgag agcagtttgc gccacggtgc taccatcagc tgccttctgt
    16021 actagcgcgc cgttataagt aatgcagtag tcgcccggct gttccatatg cagctctttc
    16081 aggtagttgt gcacacctgc atacgggcga cccgtcgtta gcacgacatt cacgccacgg
    16141 gcgcgagctg cggcaatcgc atttttaacg gcgggtgaaa tggtgtgatc gggcagcaga
    16201 agggtgccat ccatatcgat agcaatgagt ttgatagcca tgagttcccc aggtagattg
    16261 gttcctgacc catgctaacg cgattccgct caaaaatcag tacaacaccc gagggaaaag
    16321 ggggatgcaa cgcgggttgc atgctcccca tttgcttagc ggaagagttt ccctttcagc
    16381 agttccatac ctgcggaaag cagatcgtta ttggcttgtg gtgacacttc accttgcggt
    16441 gagagcgcat caataatctt cggcaattgc tctgccagta aactggaagc tgtactggta
    16501 tccacgccaa gtttttgccc gagatcggac accgcatttg tgccgagcgc cgattccagt
    16561 tgctcgccac taaccgattg attgccctgt tgattactca gccaggttga gagaatggcc
    16621 cctaagccgc cactttgcag tttttccagc agcacctgaa tgccgccctg ctcctcaacc
    16681 caacttaaaa tagcctgata tttccccgca tcgcctttca gaaaggcacc gacaacttca
    16741 tcaaaaagcc ccatgataat cacctgtaaa gcgttacgtg ttgacccaaa aagtatagat
    16801 ttgcgggatg ataattgcgg attgcagaaa taaaaagggc ggagatgatc tccgcccttt
    16861 tcttatagct tcttgccgga tgcggcgtga acgccttatc cggcctacaa aatcatgaaa
    16921 attcaataca ttgcaagatt ttcgtaggcc tgataagcgt agcgcatcag gcacgctcgc
    16981 atggttagcg ccattaaata tcgatattcg ccgctttcag ggcgttctct tcaataaacg
    17041 cacggcgcgg ttcaacggcg tcgcccatca gcgtggtgaa caactggtcg gcagcaatcg
    17101 catctttaac ggtaacgcgc agcatacgac ggctttccgg gtccatggtg gtttcccaca
    17161 gctgttccgg gttcatctcg cccagacctt tataacgctg gatggagagg ccgcgacggg
    17221 actctttcac cagccagtcc agcgcctgct cgaagctggc taccggctga cgacgctcgc
    17281 cacgttcgat aaacgcatct tcttccagca agccacgcag tttctcaccc agcgtgcaga
    17341 tacgacgata ttcgccaccg gtgataaact cgtgatccag cggatagtca gtatccacac
    17401 cgtgggtacg cacgcgaaca atcggttcaa acaggttttg ttcggcattg gtgtggacat
    17461 caaacttcca ctggctgccg tgctgttctt tgtcgttcag ttcgctgacc agcgcgttca
    17521 cccagcgggt aacggtctgc tcatcagaga ggtcggcttc cgtcagcgtc ggctgataga
    17581 taagctcttt cagcattgct ttcggataac gacgctccat gcggttgatc attttctgcg
    17641 tcgcgttgta ctcagacacc aatttctcta acgcttcgcc agccaatgcc ggtgcgctgg
    17701 cgttggtgtg cagcgttgcg ccatccagcg cgatagagat ctggtactga tccatcgctt
    17761 cgtcgtcttt aatgtactgt tcttgcttgc ctttcttcac tttgtacagc ggcggctgag
    17821 cgatgtagac gtgaccgcgt tcaacgattt ccggcatctg acgatagaaa aaggtcaaca
    17881 gcagcgtacg aatgtgcgag ccgtcgacgt ccgcatcggt catgatgatg atgctgtgat
    17941 aacgcagttt gtccgggttg tactcgtcac gaccgatacc acagccaagc gcggtgataa
    18001 gcgtcgccac ttcctgagaa gagagcatct tatcgaagcg cgctttctca acgttgagga
    18061 ttttaccctt cagcggcaga atcgcctggt tcttgcggtt acgcccctgc ttcgcagagc
    18121 cgcccgcgga gtccccttcc accaggtaca gttcggaaag cgccggatcg cgttcctggc
    18181 agtctgccag tttgcccggc aggcccgcta agtcgagcgc acctttacgg cgggtcattt
    18241 cacgcgcgcg acgcgccgct tcacgggcac gggcagcatc gataattttg ccaaccacga
    18301 ttttcgcgtc ggttgggttt tccagcaggt attctgccag cagttcgttc atctgctgtt
    18361 caaccgctga tttcacctca gaagaaacca gtttgtcttt ggtctgggag gagaatttcg
    18421 ggtccggcac tttcacggaa acgaccgcaa tcaggccttc acgcgcatcg tcaccggtgg
    18481 cgctaacttt ggcttttttg ctgtagcctt ctttgtccat gtaggcgttc agggtacggg
    18541 tcatcgccgc acggaagcct gccaggtgag taccgccgtc acgctgcgga atgttgttgg
    18601 taaagcagta gatgttttcc tggaagccat cgttccactg caacgccact tcgacgccaa
    18661 taccgtcttt ttcagtggag aagtagaaga tattcgggtg gatcggcgtt ttgttcttgt
    18721 tcagatattc aacgaacgcc ttgatgccgc cttcatagtg gaagtggtct tctttgccgt
    18781 cgcgcttgtc gcgcagacga atggaaacgc cggagttgag gaacgacaac tcacgcagac
    18841 gtttcgccag aatttcatat tcgaactcgg tcacattggt gaaggtttcg aggctgggcc
    18901 agaaacgcac catggtgccg gttttttcag tctcgccggt aaccgccaac ggtgcctgcg
    18961 gtacaccgtg ttcgtagatc tgacggtgaa ttttaccctc gcgctggata accagctcca
    19021 gtttttgcga cagggcgttt actaccgaaa caccaacgcc gtgcagaccg ccggacactt
    19081 tataggagtt atcgtcaaat ttaccgcctg cgtgcagaac ggtcatgatc acttccgccg
    19141 ccgatacgcc ctcttccggg tgaataccgg tcggaatgcc gcgcccgtca tcctgtacag
    19201 agacagagtt atcggcgtga atggtgacga taatttcttt acagtgaccc gcgagcgctt
    19261 cgtcgatagc gttatctacc acctcgaata ccatgtggtg cagaccggtg ccgtcatccg
    19321 tgtcgccgat atacataccc gggcgcttac gcaccgcatc cagccctttc aggactttga
    19381 tactggagga gtcataagaa ttcgacatca acgtttctcg ctcatttata cttgggttaa
    19441 tccgttattt tacccttttc cacggtaaac atcttcgaat tttcgtccga catgtctata
    19501 acgtgttcag cactgatcgc gctgacaaag acctgtgatt gcgtcgcttt taagcggctg
    19561 gcaagcagcc cgcgacgctc atcatcaagc tcagaggcaa aatcatctat caggtagaga
    19621 caccgccgcc cgctttcacg ggtgaggaac tctccttgcg ccagacgtaa ggcgcacatc
    19681 aacagcttaa gctgcccacg cgataaggta tcttccaccg gcgcaccgtc ggcgcgaatg
    19741 cgtaagtccg ctttatgcgg gccatgcgcg gtgtaggtta gctggcgatc gcgttcaaaa
    19801 ttacgttcca gcacctcagc atattctgtc tctttctccc agccgcgctg gaaagagaaa
    19861 gtcagagaaa actcagggag aaattgctta caagtatcgg ccatatcagc cgcgataccg
    19921 gcgctatact ccgcgcgcca ggtgctgatt tgctccgcca gcgggatcag ctctttatcc
    19981 cacgggcgta gctgttcgta acgtgtcacc tggcgcagcg ccgcattgcg ctgcttgagc
    20041 aatcgcttga gattgctcca ggcggtgaaa aatccgggtt cgttgtgaaa gcatccccag
    20101 tcgaggaatg ctcttctgta tttggggccg ccgttgagta aagtaaaccc ttctggcgtt
    20161 atcaactgca ttggcatcag gtgcgccagt tccgcgacct tatgcccgtc tgtaccgtcg
    20221 atgcggactt tgctgtcgcc ctgtttgtct ttggttaagc caatcgctgt ctcgcgctct
    20281 tcgccctgta atcgcccgtg gagaacaaac gcctcctgct catggcgaat gacgcgacca
    20341 atctgcaaac tgcgaaacgc ccgaccatgg ccgagcgtat agatggcttc cagcacgctg
    20401 gttttgccac tgccgttggc acctaccaga aagttaaagc cgggagataa agcgagatcc
    20461 gcggtttcaa tgttgcggaa atcgcggatc aacaagcggg tgagggacat tacagtctca
    20521 ttggcatgac aacataggca gccgactgtg atgcggcatc ttcaatctgc acgctggaaa
    20581 ccgaatcggt cagcatcatg cggacgtttt cacatttcag cgcgttcaga acatccagca
    20641 cgtagctgac gttgaagccg atttccatct ccgcaccgct ataggtaacg tcgaggatct
    20701 cttccgcttc ttcctgttcc gggttgttgg cggtgatttt cagctggttt tcgctgacat
    20761 aaagacgtac gccgcggaat ttctcgttag agagaatcgc cgcgcgagca aacgcctgct
    20821 tgagcagatc gcagccagct tccagatgtt tgtccgggtt cttcggcaga acgcggcgat
    20881 aatccgggaa gcgaccatcc accagtttgg aggtgaagat aaagtcgcca acgtgggcgc
    20941 gaatgttgtt gctgccaatc tgtacgcgca gcggattgtc gccgccgtcg agcatacgca
    21001 tcagttcaat cacgccttta cgcggcacga tcaccgaatg gcttggcaaa gattgaccaa
    21061 ttggcattga acagactgcc agacggtggc cgtcggtcgc cacggtgcgc agttcttcgc
    21121 cttcggtttc aaacagcata ccgtttaagt aatagcgaac gtcctgatgc gccatagaaa
    21181 actgggtcgc ttcaatcaga cgcttcatcg tcgcctgcgg cagggtaaat tcgacttcgc
    21241 tctgccagtc atcgaggttc gggaaatccg ccgctggcag ggtagacagc gaaaaacggc
    21301 tacgcccgga gcgcaccagc atccgttcac cttccagctg tacggcaatt tccgcgcctt
    21361 caggcagacc acggcagata tcaaagaatt tgcgcgccgg aacggtcgtc gctcctggct
    21421 cgtgtggctg aaccagcgca acgcgtgcca ccatttccat ctcgagatca gtaccggtca
    21481 gcgacaacgt accgtcagca acctgtaaca gcagattacc gagaatcggt agcgtaggac
    21541 gaccacctaa cggcccgctc acctgttgta gcggttttaa taaatgctca cgttctacgg
    21601 taaatttcat aggtttacga tgacaatgtt ctgattaaat ttgaaaaatc ttctttgata
    21661 tcgtggctct cttcacgcaa ctgctcgatc ttacggcagg catgaagcac cgtcgtgtgg
    21721 tcacgaccac caaacgcatc gccaatctcc ggcagactgt ggttagtcag ctctttcgcc
    21781 agcgccatcg ccatctggcg cggacgcgcc accgagcggg atcgacgctt ggaaaggaga
    21841 tccgcgactt tgatcttgta gtactccgcc accgtcttct gaatattgtc gatggtgacc
    21901 agtttttcct gcaatgccag caagtcgcgc agtgcctcac gcacgaagtc gatggtgatc
    21961 gcccgtccgg taaagttggc gttagcaata acgcggttca gcgccccttc cagctcacgt
    22021 acgttagatc gtagacgctt ggcgataaag aacgccactt cgcctggcaa acgaatgtca
    22081 ttttcgtcgg cctttttcat caggatcgcc acgcgggttt ccagctctgg cggttcgatc
    22141 gccacagtca gtccccaacc gaagcgggat ttcaaacgat cctcaacgcc gttgatctct
    22201 ttcggatagc gatccgaggt gagaatgatc tgttgattac cttccagcag ggcgttgaag
    22261 gtgtggaaaa actcttcctg agatcgttct ttattagcaa aaaactgaat atcgtcgatc
    22321 agcagtgcat ctacggaacg gtagtagcgt ttaaactctt cgatcgcgtt gttttgcagg
    22381 gctttaacca tgtcctgaac aaagcgctcg gagtgcatat aaaccacttt ggcattcggc
    22441 ttgcgcgcca taatgccgtt acccaccgca tgcagcaggt gagttttacc cagacccgtg
    22501 ccgccataaa ggaacaatgg gttataggca ccgccagggt tatccgccac ctggcgagcc
    22561 gccgcgcgcg ccagttggtt agatttacct tcaacgaagt tatcaaacgt gtgtttgacg
    22621 tttacgttag aacgataggt cggttctgcc ggggccggga cgttatccca acctgagcgc
    22681 gtagaaggcg cagcacgttg cggctgcgtt tgcgccacct gtgcaggggc cgcgacgttg
    22741 ctcgtcactg ccgcttgtgg cgtttgcgtc accggtttgg tgccgacttc aaaacgcagc
    22801 tgtggggcat ccgctccgca gaaactggtt agcagtccat tgatattatt aaggtacttg
    22861 tcccgtaccc aatcgaggac aaaacggttt ggcgcgtaca gggccagcgt gttatcgctc
    22921 agttccgcct gcaatgggcg tatccacata ctgaattctg tggctggtaa ctcatcctgc
    22981 aatcgggcaa gacactgctg ccaaagcgaa agtgacacgg cggactccac tcgaacaaaa
    23041 gtcgataatg actaaggctg aaacattcat gattgttgac gtacgtcgaa aagaccctgc
    23101 ttgagggtga cgcacgaacc gctgtctgcg gttatatgcc cgatcaagat cctgcaaaac
    23161 gatcgggacc gcggatcata gcctaaactg cgcaagagat cttctgtttc tcacagattt
    23221 tcccgattta tccacaggac tttccagacc tcgctaagtg taaacgatcc tgccgcgagg
    23281 cgggcacgat ttacgccgca tattggaaaa tttaatgacc atagacaaaa attggcttaa
    23341 tcgatctaat aaagatctag gacgatcctt gcgctttacc catcagcccg tataatcctc
    23401 cacccggcgc gccatgctgg ttttcactgg tgtgaggtcg tacattttcc ctgcgaaaag
    23461 gtgcggaaaa gcgcggtaaa taaggaaaga gaattgactc cggagtgtac aattattaca
    23521 atccggcctc tttaatcacc catggcttcg gtgtccatcg tttcattttt cggcggatat
    23581 ccaataaagc cattgaattt attcaagttt aggtagaaat cgccatgaaa cgcacttttc
    23641 aaccgtctgt actgaagcgc aaccgttctc acggcttccg tgctcgtatg gctactaaaa
    23701 atggtcgtca ggttctggca cgtcgtcgtg ctaaaggccg cgctcgtctg accgtttcta
    23761 agtaataaag ctaacccctg agtggttaag ctcgcatttc ccagggagtt acgcttgtta
    23821 actcccagtc aattcacatt cgtcttccag cagccacaac gggctggcac gccgcaaatt
    23881 accattctcg gccgcctgaa ttcgctgggg catccccgta tcggtcttac agtcgccaag
    23941 aaaaacgttc gacgcgccca tgaacgcaat cggattaaac gtctgacgcg tgaaagcttc
    24001 cgtctgcgcc aacatgaact cccggctatg gatttcgtgg tggtggcgaa aaaaggggtt
    24061 gccgacctcg ataaccgtgc tctctcggaa gcgttggaaa aattatggcg ccgccactgt
    24121 cgcctggctc gcgggtcctg atagccctca ttcgggtcta tcaacgcctg attagtccgc
    24181 tactcgggcc gcattgtcgt ttcactccaa cctgttcaag ctacggaatt gaggcattgc
    24241 gcaggtttgg agtgataaaa ggcagttggt tgacggtgaa acgcgtatta aaatgccacc
    24301 ctttacaccc tggtggtgac gatcccgtcc cgcccggacc atttgatacc agagaacact
    24361 aacgatggat tcgcaacgca atcttttagt catcgctttg ctgttcgtgt ctttcatgat
    24421 ctggcaagcc tgggagcagg ataaaaaccc gcaacctcag gcccaacaga ccacgcagac
    24481 aacgaccacc gcagcgggta gcgccgccga ccagggcgta ccggccagtg gccaggggaa
    24541 actgatctcg gttaagaccg acgtgcttga tctgaccatc aacacccgtg gtggtgatgt
    24601 tgagcaagct ctgctgcctg cttacccgaa agagctgaac tctacccagc cgttccagct
    24661 gctggaaact tcaccgcagt ttatttatca ggcacagagc ggtctgaccg gtcgtgatgg
    24721 cccggataac ccggctaacg gcccgcgtcc gctgtataac gttgaaaaag acgcttatgt
    24781 gctggctgaa ggtcaaaacg aactgcaggt gccgatgacg tataccgacg cggcaggcaa
    24841 cacgtttacc aaaacgtttg tcctgaaacg tggtgattac gctgtcaacg tcaactacaa
    24901 cgtgcagaac gctggcgaga aaccgctgga aatctccacc tttggtcagt tgaagcaatc
    24961 catcactctg ccaccgcatc tcgataccgg aagcagcaac ttcgcactgc acaccttccg
    25021 cggcgcggcg tactccacgc ctgacgagaa gtacgagaaa tacaagttcg ataccattgc
    25081 cgataacgaa aacctgaaca tctcttcgaa aggtggttgg gtggcaatgc tgcaacagta
    25141 tttcgcgacg gcgtggatcc cgcataacga cggtaccaac aacttctata ccgctaatct
    25201 gggtaacggc atcgccgcta tcggctataa atctcagccg gtactggttc agcctggtca
    25261 gactggcgcg atgaacagca ccctgtgggt tggcccggaa atccaggaca aaatggcagc
    25321 tgttgctccg cacctggatc tgaccgttga ttacggttgg ttgtggttca tctctcagcc
    25381 gctgttcaaa ctgctgaaat ggatccatag ctttgtgggt aactggggct tctccattat
    25441 catcatcacc tttatcgttc gtggcatcat gtacccgctg accaaagcgc agtacacctc
    25501 catggcgaag atgcgtatgc tgcagccgaa gattcaggca atgcgtgagc gtctgggcga
    25561 tgacaaacag cgtatcagcc aggaaatgat ggcgctgtac aaagctgaga aggttaaccc
    25621 gctgggcggc tgcttcccgc tgctgatcca gatgccaatc ttcctggcgt tgtactacat
    25681 gctgatgggt tccgttgaac tgcgtcaggc accgtttgca ctgtggatcc acgacctgtc
    25741 ggcacaggac ccgtactaca tcctgccgat cctgatgggc gtaacgatgt tcttcattca
    25801 gaagatgtcg ccgaccacag tgaccgaccc gatgcagcag aagatcatga cctttatgcc
    25861 ggtcatcttc accgtgttct tcctgtggtt cccgtcaggt ctggtgctgt actatatcgt
    25921 cagcaacctg gtaaccatta ttcagcagca gctgatttac cgtggtctgg aaaaacgtgg
    25981 cctgcatagc cgcgagaaga aaaaatcctg attcggtgag ttttcgctaa aataagggcg
    26041 gtcagttgac cgcctttttt cttttcgtag ggtggataag caccgcgcat ccgccacaca
    26101 aagcaacagg aacatcatga gcgataatga cactatcgta gcccaggcca cgcctccggg
    26161 acgtggcggc gttggcatcc tgcgcatctc cggcctaaaa gcccgtgaag ttgccgaaac
    26221 cgtgctgggt aaactgccta agccgcgcta cgccgattat cttccgttta aagacgccga
    26281 cggcagcgtg ctcgatcagg ggattgcgct atggttccct ggcccgaact cgttcaccgg
    26341 cgaagatgtg ctggaactgc agggtcatgg cggtccggtg atcctcgacc tgctgttaaa
    26401 acgcattctg accattcccg gcctgcggat tgctcgccct ggtgagtttt ccgaacgcgc
    26461 gtttcttaac gataaactcg acttagccca ggccgaggcg attgccgatc ttatcgacgc
    26521 cagttcggaa caggcggccc gttcggcact taactcgctg caaggcgcat tctccgcacg
    26581 ggtcaaccat ctggtggaag ccctcaccca cttgcgcatt tacgtcgaag cggcaattga
    26641 tttccctgat gaagagatcg atttcctctc cgacggcaaa attgaagccc agcttaatga
    26701 cgtgattgcc gatctcgatg cagtgcgtgc tgaagcacgt cagggtagtt tgttgcgcga
    26761 agggatgaag gtggtgattg ccggacgtcc taacgccggt aaatcgagcc tgttaaacgc
    26821 gctggcgggc cgtgaagcag caatcgtaac tgatatcgcc ggaaccacac gtgacgtgct
    26881 gcgtgagcat atccacattg acggaatgcc gctgcatatc atcgataccg ctgggctacg
    26941 tgaagccagt gacgaagtgg aacgtattgg tatcgagcgc gcgtggcagg aaattgaaca
    27001 ggccgaccgc gtgctgttta tggtcgatgg caccacaaca gacgccgtgg atccggcaga
    27061 gatctggccg gaatttatcg cccgtctgcc agcgaaacta ccgatcaccg tggtgcgcaa
    27121 taaagccgat atcaccggcg aaacgctggg aatgagtgaa gtgaacggtc acgcgttaat
    27181 tcgtctctcg gcaaggacag gtgaaggcgt ggaggtgctg cgtaaccatc tcaaacagag
    27241 catgggcttt gacaccaaca tggaaggcgg cttcctggcg cgtcgtcgcc acctacaggc
    27301 gctggaacag gcagcggagc atttgcagca aggtaaagcg caactgttag gtgcctgggc
    27361 gggtgaactg ctggcggaag agttgcgcct ggcacagcag aacttaagcg aaatcaccgg
    27421 ggaatttacc tctgatgatt tgctggggcg gattttctcc agcttctgta ttggtaagta
    27481 accgcgctta cgaagccgta ttctgactgt cagatgcggc ttcgcttcat tgttagcact
    27541 cctgttattc ctcaaccctt tttttaaaca ttaaaattct tacgtaattt ataatcttta
    27601 aaaaaagcat ttaatattgc tccccga
//
LOCUS       DAJVQG010000040        27264 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJVQG010000040 DAJVQG010000000
VERSION     DAJVQG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27264
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.040"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..1438)
                     /gene="rsmF"
                     /locus_tag="OQM83_001555"
     CDS             complement(<1..1438)
                     /gene="rsmF"
                     /locus_tag="OQM83_001555"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCS1519842.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1519..4152)
                     /gene="yebT"
                     /locus_tag="OQM83_001556"
     CDS             complement(1519..4152)
                     /gene="yebT"
                     /locus_tag="OQM83_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCS1519843.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4121..5404)
                     /gene="yebS"
                     /locus_tag="OQM83_001557"
     CDS             complement(4121..5404)
                     /gene="yebS"
                     /locus_tag="OQM83_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCS1519844.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5534..6031
                     /gene="msrC"
                     /locus_tag="OQM83_001558"
     CDS             5534..6031
                     /gene="msrC"
                     /locus_tag="OQM83_001558"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCS1519845.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6128..6826
                     /gene="proQ"
                     /locus_tag="OQM83_001559"
     CDS             6128..6826
                     /gene="proQ"
                     /locus_tag="OQM83_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCS1519846.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDALRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            6846..8894
                     /gene="prc"
                     /locus_tag="OQM83_001560"
     CDS             6846..8894
                     /gene="prc"
                     /locus_tag="OQM83_001560"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCS1519847.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9086..9967
                     /gene="htpX"
                     /locus_tag="OQM83_001561"
     CDS             9086..9967
                     /gene="htpX"
                     /locus_tag="OQM83_001561"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCS1519848.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10013..11076)
                     /locus_tag="OQM83_001562"
                     /pseudo
     CDS             complement(10013..11076)
                     /locus_tag="OQM83_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11253..12044
                     /gene="kdgR"
                     /locus_tag="OQM83_001563"
     CDS             11253..12044
                     /gene="kdgR"
                     /locus_tag="OQM83_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCS1519849.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12187..12426)
                     /locus_tag="OQM83_001564"
     CDS             complement(12187..12426)
                     /locus_tag="OQM83_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1519850.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12585..12728
                     /gene="mgrB"
                     /locus_tag="OQM83_001565"
     CDS             12585..12728
                     /gene="mgrB"
                     /locus_tag="OQM83_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCS1519851.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            12803..13090
                     /locus_tag="OQM83_001566"
     CDS             12803..13090
                     /locus_tag="OQM83_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCS1519852.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            13760..13903
                     /locus_tag="OQM83_001567"
     CDS             13760..13903
                     /locus_tag="OQM83_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCS1519853.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            13916..14125
                     /gene="cspE"
                     /locus_tag="OQM83_001568"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             13916..14125
                     /gene="cspE"
                     /locus_tag="OQM83_001568"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCS1519854.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            14291..15100
                     /gene="rlmA"
                     /locus_tag="OQM83_001569"
     CDS             14291..15100
                     /gene="rlmA"
                     /locus_tag="OQM83_001569"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCS1519855.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(15097..15663)
                     /gene="mntP"
                     /locus_tag="OQM83_001570"
     CDS             complement(15097..15663)
                     /gene="mntP"
                     /locus_tag="OQM83_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCS1519856.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(16092..16550)
                     /locus_tag="OQM83_001571"
     CDS             complement(16092..16550)
                     /locus_tag="OQM83_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCS1519857.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(16605..17456)
                     /gene="manZ"
                     /locus_tag="OQM83_001572"
     CDS             complement(16605..17456)
                     /gene="manZ"
                     /locus_tag="OQM83_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCS1519858.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(17469..18269)
                     /gene="manY"
                     /locus_tag="OQM83_001573"
     CDS             complement(17469..18269)
                     /gene="manY"
                     /locus_tag="OQM83_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCS1519859.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(18332..19303)
                     /gene="manX"
                     /locus_tag="OQM83_001574"
     CDS             complement(18332..19303)
                     /gene="manX"
                     /locus_tag="OQM83_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCS1519860.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            19766..21316
                     /gene="yoaE"
                     /locus_tag="OQM83_001575"
     CDS             19766..21316
                     /gene="yoaE"
                     /locus_tag="OQM83_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCS1519861.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(21320..22918)
                     /locus_tag="OQM83_001576"
     CDS             complement(21320..22918)
                     /locus_tag="OQM83_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1519862.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(23049..24413)
                     /gene="sdaA"
                     /locus_tag="OQM83_001577"
     CDS             complement(23049..24413)
                     /gene="sdaA"
                     /locus_tag="OQM83_001577"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCS1519863.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(24597..25175)
                     /locus_tag="OQM83_001578"
     CDS             complement(24597..25175)
                     /locus_tag="OQM83_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCS1519864.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(25179..26540)
                     /gene="pabB"
                     /locus_tag="OQM83_001579"
     CDS             complement(25179..26540)
                     /gene="pabB"
                     /locus_tag="OQM83_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCS1519865.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            26614..26793
                     /locus_tag="OQM83_001580"
     CDS             26614..26793
                     /locus_tag="OQM83_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCS1519866.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            complement(26913..27236)
                     /locus_tag="OQM83_001581"
     CDS             complement(26913..27236)
                     /locus_tag="OQM83_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCS1519867.1"
                     /translation="MVNFKDKNMPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKY
                     LKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEE
                     LKALV"
BASE COUNT         7029 a         6712 c         6890 g         6633 t
ORIGIN      
        1 aggcgttacc ggtaaaaagt ttcccatcgc gcaccagttc acgcggatag ctgtttttca
       61 atcgcgaacc aatccgtttg gctaaaccaa tcggttgatg ctggaaagta accaacacgt
      121 catccgccac tggcgcggct tgcgggtaaa catcgcgccc gcgataccac tcctccgctt
      181 cctgcggtgt cagttcaaaa gcgttcacat tgtcgggggt ggcaagggca ataactgctt
      241 catgctgcca gcgataacct ttgttgtgcg tttcggcaag tttaatcccc aagcgagaaa
      301 atcggacttt accgatcagg gcttcaatgc ccaccgggaa caaccacagt tctttgtcac
      361 gctgccagag gcgcaggttt tcatcccagt ttaagccaac acctgcagcc gcctgacgaa
      421 tttgtccagc ttctcgatct ttcactgggc tgaacggaaa attaccgact ttgtatttgg
      481 gggcgggtaa ggcgggaatc gcctgagttt tacgcagacg agcaacgaag aagccttcgc
      541 agtcgtaaat ttgtgggaaa acatgcaaaa agccttcctc ggtcagcgcc ttgtttgcac
      601 cagggaagag atcaccaagc ggtaaaaact ctactgcgtc ggggtaagtc tctttcagcc
      661 acaggcaaac ggcttcgttt tcttcctggt ttaaggtaca ggtcgagtaa accagcgtac
      721 cgccagggcg taatgcatga aaggcgctgt cgatcagttc ccgttgggta gctgcgattt
      781 cctgattgct ttctggtgac cagtttttta gcgcatcggg atctttacgc accacgcctt
      841 cgccggagca gggagcgtcc agcaaaatgg catcaaacat ttctggcact gccgcaccaa
      901 acacgcggcc atcaaaatgt gtgagcgcaa cattactgat accacagcgg ctgatattgg
      961 catgtaacac ttttacccga ctggcggaaa actcattggc aagaattgcc ccttcgttat
     1021 tcatccgcgc ggaaatttgc gtcgttttgg agccaggcgc ggcagcgaca tccatcaccc
     1081 gctgtggtgc attaccgtca gcaaacaagg cggcgacggg caacattgaa ctggcttcct
     1141 gaatataaaa caggccactt aaatgctcgg cggtactacc caatggcaat gcatcttcat
     1201 tgtcgcgttc aatccagaaa ccttcttcac accacggaat tggcgtaagc gtccagccat
     1261 aaggagcggt taattgcagg aaatcagcaa cggagatttt cagcgtatta acgcgaatgc
     1321 tgcggcgcaa cgggcgctga caggcggcaa gaaaatcatc aaaagagagc gtcgaaggca
     1381 tcgcttcgcg catttgcgtc agaaaggcgt ccgggaaata aacggtgtgt tgggccacga
     1441 gcatatacca ctggcaaaaa aaacaggcgc gcagtttagc ataaacgctc cggcgcaggc
     1501 acgccggagc agtgggcatt atttgggaag cgcagtaccc cattcacgcc actctttcgg
     1561 ttcactttcc tgcaacagga agtgcttgcc ttcctgggct ttcggtgcca gtggcgtacc
     1621 cggaggcgtg gcgaaggcga tgccgccacg gataaactgg ttaaaggtgc cggttttcac
     1681 tacgccgccc gtcagaccaa agtccagact gtaacccgat gccaaccaga agacggaatt
     1741 gttacgcacc aggtgttgat agcgtttact gatgcgcatc gcaatcatca cgcgatctga
     1801 caatgtcccc agcgtcattc cggtaaccgt acctacttcc agaccacgga acagcacagg
     1861 cgtaccaata cctaacgaac cggcttccgg tgcttcgaca ataatgctta agccatccag
     1921 gtaacgcgaa tcagtaatag tagcttcttg caactcaaag tcacggcgag gattaccccg
     1981 acctggttcg acattgatat acggctggag gatagtatct agatgctcaa cgcccgcggc
     2041 tgaaatttgc ggtgtgacca ccgagaagcg cgtaccgccg cgggcgaagg tctggacata
     2101 ctccggatag agcaccgcct ttgcctgtac ttcattgcgc gcggtaatca gatccagcgt
     2161 ctggatttgc ccgatatcaa taccgagata gcgaattggc atcccgaccg ccagttttcc
     2221 ggcatcgaaa gcgtgaagcg taatctgccc accaaccgca cgggccgctg tttcggaagc
     2281 atacagaatt cgtttgtcgc ctttacgctg actggcgctg gcaccgctga ggttatcgaa
     2341 gctaatggct ccctttaatg ctctggagag cggggatgcc tgtacggtca ggccactacc
     2401 attcagctga actttcgccc cgccttctgc ccagaacacg ctattgctgg tcagaaggtt
     2461 gcgatactcc ggcttaatat gcagatcgat atcaaacgcg ttagctcgcg gacgcacggt
     2521 aatcacttca ccaacttcaa atttacggta cagcactacc gatcctgcct gcacatccgg
     2581 cagcgtctct gcactcaaac tcacggttgt ggtgggtaaa tcgctaaggc tgttctccag
     2641 cgctttttcc agattggcat acagtggata gctggctttc atctcacctt tatcgcctgg
     2701 cagaatacgt atcccgccgt taatccattc tgaggcgctg gcaccaagaa actcaacgcc
     2761 atccagcccc accttcacgt cgacacggct gttgacgaca aatttgctat cgccttttac
     2821 cagttcgcga tgctgaggct cgatggcgac ggtaaaggta acgcctttgc tggtgagttt
     2881 acgatcaata acctggccta cctgcacgcc gtgaagaatg agcggctgac cagcatcaat
     2941 accgtaactt tccggcgcgg tcagggtcag cgtcagaaca tcaggttcat gcagcagtgc
     3001 tttttcgcct ggcacaacaa cgaactcttt gcgtggctcg ccatcgccgg gtaccaactc
     3061 gaaggttttg ccagtcagca gggcgctgag attggcatcg ctaagggata atttcggatt
     3121 aagtaattcg atgcgggtat tttcacgaag cagggtaacg acgctgggat taactgtcat
     3181 ttccccagtg actttaccac caggatttaa atccagttta gttagctgtc cgacttccag
     3241 tccctgatac attaatggcg tcgagtcggc ggttaatccg gccccactcg gcagttccag
     3301 tttgattatt acgccacgct ggctatgggc gaggtcttca tacagaccaa aggtatcttc
     3361 cgcctcggca ggtttcgact cttcaggtga atcgaaggca atcgcaccgt taaccagtgc
     3421 cgccagactt tccagtttca ccttcgcacc actgatactg acgttggcat caacgccgga
     3481 aacgttccag aaacggctac cttttttcac taggtcggta aaacgccgct cgatcaggac
     3541 atcaatcacc acgccttgct tgttgggatt gatggcatag tcgtagactt ttcccacagg
     3601 gatcttgcgg aaatagacca atgaaccgct gttcagcgaa ccgagatcgg gggcttgcag
     3661 gtggatcatc agatcgccat tgtccagtcg atatttcggt tgggtatcga gtgcgacaaa
     3721 gtgatcctgc tctttacctt tacctggcat catgccgata tagttaccac cgacgagggc
     3781 gtccagcccg gagacacctg ccaacgatgc ttttggcgtc accagccaga actgagtctc
     3841 ttcgcgcagc gcatctttca tatcggactt gatgctgacc ttgacttcaa tcttacgaag
     3901 atcgtcgctg aggctgatat cctgcactgt tccgacttca acgccctgat aacgaacagg
     3961 cgtacggccc gggacaatac catccgccga cataaagtcg atggtgacgg tattaccccg
     4021 gtcctgataa ctgtcccaaa tcagccaacc ggcaatcatt agcgcgatga aaggcagcag
     4081 ccagaaaggt gagatacggc gtttattttt aatctgcgct tcagtcgtcg aagcgggcgt
     4141 ttcctgactc atgtgcatcc caaagtaagc ggctgtccag ccattccaca gcaagaatag
     4201 tcaaaattac ggctgcgcca aaataaaacg cagccggtcc catagtaaaa gcgaggatct
     4261 gatcgcgatt aatcagcgac atggttaaag atatgacaaa caggtcgagc atcgaccagc
     4321 gtccaatcca ggtcaccatc cgcagtaaca gaatgcgtgt gcgtaaacct tgctggcatt
     4381 taaaatgaat gctcaacagt aaagtgaaca tgacgatcac tttagtaaac ggtaccagaa
     4441 tactggcgat aaacacgatt ccggcgaccg caatgttgct acttgccagc gacataatcc
     4501 cggaaagaat cgtatcttcc tgccggccac cattcagata aatgatagaa ataggcaata
     4561 ggttggctgg taacaacaaa acgattgacg ctaacagcgc cgcccagcat ttttgcaaac
     4621 tatgacgacg acgcaggcgt agcgggatat ggcaacgcgg gcagcgacca cgctgatcag
     4681 gatagccggt aaaatggcac ccaagacaga cacgaagttt ctcgtcccta cgcgtagcgg
     4741 ggcgctgcgg ataatatcgc tcccacagct cctcaacatt aagatgtgac aacgtcaccg
     4801 tcgttaaaat caccaacgcc acaaaagaga acaagccgac acccgcctgg atatgggcat
     4861 aatcctgtac ctttatagaa gcaacgccaa tgccgaccag gtagatatcc agcattaccc
     4921 actctttaag acgctcaagc atcagcagta ccggacgtaa attcattccc agtcggttac
     4981 caaaccataa atatgctatg gaggacacca gaatgagggg ggcaccaata acgcagaaaa
     5041 agaccatcgc ccccgttatc gtatcgccct gtttggtcat ttgccagatg ccttgcatga
     5101 cgttggcgtc gatacgaata cctaacagcc agatatgcaa cagcggttcg ccccaggcaa
     5161 acggcatcag caacagcatg gtgaaagcca ttgccgccag gcgcgttagc gaccagtcgc
     5221 gcccgtcgcg aatttttgcc tgacagcgcg gacaataggc actctgatga gaatttatct
     5281 cgggcaggct aaacagcatg tcacattgcg ggcaacgttg gtaatcacca cgcggcagtt
     5341 cctcgccgat tgccctcact gttatctttt ttgtcggcgt aatttgtggt gtgttaagag
     5401 ccatgacgtg ccaaatgtaa taagaattaa cattaatctt aacccatgat gggaatgatc
     5461 ttgagcatta acgcatttaa tgcttatttt aataggctgg cagttccagg taagacaaac
     5521 aagtgattat ataatgaaca aaacagaatt ttacgcggat ttaaatcgcg actttaacgc
     5581 gctgatggcg ggagaaacca gttttctggc aacgcttgcg aacaccagtg cgttgttata
     5641 cgagcgtctc actgacgtaa actgggcagg tttttatttg cttgaggacg atacactggt
     5701 actcggacca tttcagggca aaattgcctg tgtccggata cccgtcgggc gcggggtgtg
     5761 cggcactgcg gttgcccgca atcaagtgca gcgtatcgag gatgttcacg cgtttgacgg
     5821 gcatattgcc tgtgatgcgg cgagtaattc tgaaattgtt ctgccgctgg tggtgaaaaa
     5881 tcagattatt ggtgttcttg acatcgatag taccgtcttc ggtcgcttta cagacgagga
     5941 cgagcaaggc ttacgtcagc ttgtggcaca gcttgaaaaa gtgcttgcaa cgacggatta
     6001 caaaaaattc tttgcgagcg tcgcaggata atcaacggat aacgtagcat ttactggtag
     6061 cgtcattata atgacgcctg ttcatgcctg cgcttgttgg ctacgtccgt tgtaatcagg
     6121 aaatttcatg gaaaatcaac ctaagttgaa tagcagtaaa gaagtaatcg cgtttctggc
     6181 cgaacgtttt ccccactgtt tcagtgcgga aggtgaagcg cgtccgctga aaatcggtat
     6241 ttttcaggat ttggtcgatc gtgttgccgg ggaaatgaac ctgagcaaaa cgcaattacg
     6301 atccgcttta cgtctctaca cttcgagctg gcgttatctt tacggtgtta aacccggcgc
     6361 aacgcgtgtc gatcttgacg gcaacccatg cggtgagctg gacgagcaac atgtagagca
     6421 tgctcgcaag cagcttgaag aagcgaaagc gcgtgttcag gcacagcgtg ctgaacagca
     6481 agcgaaaaaa cgcgaagctg ccgcagctgc tggtgagaaa gaagacgcac tgcgccgcga
     6541 acgcaagcca cgtccgacca cgccacgccg caaagaaggc gctgaacgta aacctcgtgc
     6601 gcaaaagccg gtagagaaag cgccaaaaac agtaaaagca cctcgcgaag aacagcacac
     6661 cccggtttct gacatttcag ctctgactgt cggacaagcc ctgaaggtga aagcgggtca
     6721 aaacgcgatg gatgccaccg tattagaaat caccaaagat ggcgtccgcg tccagctgaa
     6781 ttcgggtatg tctttgattg tgcgcgcaga acacctggtg ttctgaaacg gaggccgggc
     6841 caggcatgaa catgtttttt aggcttaccg cgttagctgg cctgcttgca atagcaggcc
     6901 agaccttcgc tgtagaagat atcacgcgtg ctgatcaaat tccggtatta aaggaagaga
     6961 cgcagcatgc gacggtaagt gagcgcgtaa cgtcgcgctt cacccgttct cattatcgcc
     7021 agttcgacct cgatcaggca ttttcggcca aaatctttga ccgctacctg aatctgctcg
     7081 attacagcca caacgtgctg ctggcaagcg atgttgaaca gttcgcgaaa aagaaaaccg
     7141 agttaggcga tgaactgcgt tcaggcaaac tcgacgtttt ctacgatctc tacaatctgg
     7201 cgcaaaagcg ccgttttgag cgttaccagt acgctttgtc ggtactggaa aagccgatgg
     7261 atttcaccgg caacgacact tataaccttg accgcagcaa agcgccctgg ccgaaaaacg
     7321 aggctgagtt gaacgcgctg tgggacagta aagtcaaatt cgacgagtta agcctgaagc
     7381 tggcaggaaa aacggataaa gaaattcgtg aaaccctgac acgccgctac aaatttgcca
     7441 ttcgtcgtct ggcgcaaacc aacagcgaag atgttttctc gctggcaatg acggcgtttg
     7501 cgcgtgaaat cgacccgcat accaactatc tttccccgcg taataccgaa cagttcaaca
     7561 ctgaaatgag tttgtcgctg gaaggtattg gcgcagtgct gcaaatggat gatgactaca
     7621 ccgttatcaa ttcgatggtg gcaggtggtc cggcagcgaa gagtaaagct atcagcgttg
     7681 gtgacaaaat tgtcggtgtt ggtcaaacag gcaagccgat ggttgacgtg attggctggc
     7741 gtcttgatga tgtggttgcc ttaattaaag ggccgaaggg cagtaaagtt cgtctggaaa
     7801 ttttacctgc tggtaaaggg accaagaccc gcactgtaac attgacccgt gaacgtattc
     7861 gtctcgaaga ccgcgcggtt aaaatgtcgg tgaagaccgt cggtaaagag aaagtcggcg
     7921 tgctggatat tccgggcttc tatgtgggtt tgacagacga tgtcaaagtt caactgcaga
     7981 aactggaaaa acagaatgtc agcagtgtga tcatcgacct gcgtagcaat ggcggtgggg
     8041 cgttgaccga agcggtatcg ctctcaggtc tgtttattcc tgcgggtccc attgttcagg
     8101 tccgcgataa caacggcaag gttcgtgaag acagcgatac cgacgggcag gtgttctata
     8161 aaggcccgct ggtggtactg gttgaccgtt tcagtgcttc ggcttcagaa atctttgccg
     8221 cggcaatgca ggattacggt cgtgcgctgg ttgtgggtga accgacgttt ggtaaaggca
     8281 ccgttcagca ataccgttca ttgaaccgta tttacgatca gatgttacgt cctgaatggc
     8341 cagcgctggg ttctgtgcag tacacgatcc agaaattcta tcgcgttaac ggcggcagta
     8401 cgcaacgtaa aggcgtaacg ccagacatca tcatgccgac gggtaatgaa gaaacggaaa
     8461 cgggtgagaa attcgaagat aacgcgctgc cgtgggatag cattgatgcc gcgacttatg
     8521 tgaaatcagg cgatttaacg gcctttgggc cggagctgct gaaggaacat aatgcgcgta
     8581 tcgcgaaaga tcctgagttc cagaacatca tgaaggatat cgcgcgcttc aacgctatga
     8641 gggacaagcg caatatcgtt tctctgaact acgctgtgcg tgagaaagag aataatgaag
     8701 atgatgcgac gcgtctggcg cgtttgaacg aacgctttaa acgcgaaggt aaaccggagt
     8761 tgaagaagct ggatgatcta ccgaaagatt accaggagcc ggatccttat ctggatgaga
     8821 cggtgaatat cgcactcgat ctggcgaagc ttgaaaaagc cagacccgcg gaacaacccg
     8881 ctcccgtcaa gtaatatcaa tcaggcacaa gaaattgtgc ctgatttttt aacagcggca
     8941 agatgccgta aatcagatgc tacaaaatgt aaagttgtgt ctttctggtg acttacgcac
     9001 tatccagact tgaaaatagt cgcgtaaccc atacgatgtg ggtatcgcat attgcgtttt
     9061 gttaaactga ggtaaaaaga aaattatgat gcgaatcgcg ctcttcctgc taacgaacct
     9121 ggccgtaatg gtcgttttcg ggctggtact gagcctgaca gggatacagt cgagcagcgt
     9181 tcaggggctg atgatcatgg ccttgctgtt cggttttggt ggttccttcg tttcgcttct
     9241 gatgtccaaa tggatggcat tacgatctgt tggcggggaa gtgatcgagc aaccgcgtaa
     9301 cgaaagggaa cgttggctgg tcaatactgt agcaacccag gctcgtcagg cagggatcgc
     9361 tatgccgcaa gtggctatct accatgcgcc ggacatcaac gcttttgcaa ccggtgcgcg
     9421 ccgtgatgcc tctctggttg ctgtcagcac cggtttgctg cagaacatga gcccggatga
     9481 agccgaggcg gtaattgctc acgaaatcag ccacatcgcc aatggtgata tggtcaccat
     9541 gacgctgatt cagggcgtgg tgaacacctt cgttatcttt atttcccgta ttctggcgca
     9601 gcttgccgcg ggttttatgg gcggaaatcg tgatgaaggt gaagagagca acggcaaccc
     9661 gctgatctac tttgcggttg caacggttct ggaactggtg tttggtattc tggcgagcat
     9721 tatcaccatg tggttctcgc gtcatcgtga attccatgct gatgccggtt cggcaaaact
     9781 ggttggtcgc gagaaaatga ttgccgcgct gcagcgcctg aaaaccagct atgaaccgca
     9841 agaagcaacc agcatgatgg ctttctgcat taacggtaag tcgaaatcgc tcagtgagtt
     9901 gttcatgact cacccgccgc tggataaacg aattgaagct ctgcgtacgg gtgaatacct
     9961 gaagtaatat gaagtaaata ctaaaaagcg cgtcctgatc gacgcgcttt ttttatgcct
    10021 tggatcgtgg ctgagtgata cgtaaaccac tgacacaggc agcaatcact gccagaatag
    10081 ccgcagccat cagcgaaacg tgcgtaccat tatcaccgaa ctgatttagc atcagcgcca
    10141 ccagtgccgc gccgctactc tgacccagta gacgagccgt tcctaacatg ccactggctc
    10201 caccgctacg ttcgcgagga gcggaggtaa taatggtgtg gttattgggt gactggaaca
    10261 agccaaatcc agcgccacat aagatcatcg gccagataat attgatatcc gcaggtgacg
    10321 cgggcagcag aaccagggga aaaagccccg cagccatgat gaacaaccct agagcaccca
    10381 gcaatcctgc atgtacgcgt tcaatcaaat agcctgccaa cggagccatc accatcgttg
    10441 ctaacggcca cggtgtcaga agtaaacctg tttcgacttc actacgcccg agcacggttt
    10501 gcaggtaaaa gggcagggaa accattgcca gcatttgtgc gcagaaagag caaacagatg
    10561 tgcaaataga aagtgaaaac agcgggatac gcagtaaatc caccggtagc agcggtacgg
    10621 gaagagaaag ctggcggcga ataaagaaaa taccaacaac aaccattacc accagttccg
    10681 cagcaattaa cgtcagcgat tgcccctgag cgaaaccact caacgcagtg ataagcaggc
    10741 cgaaggttaa cgcgttcatc acggcgctgg gcaggtcgaa acggggttta ctggcgcgag
    10801 aaccattggg tggcagaaaa cgcatcgcca gaagcagggc gataatacct aacggtacgt
    10861 tgattaaaaa taaccatttc caggatgcga tggagaggat tgctgcagca attgtcggcc
    10921 cggcagcaga agagacggca acaatatggc gattgttggc agggcgacgt tggcgattgc
    10981 gccgtcaagg acagccatcg aaatgccaat cacaatggtt aatatcgcac cgtatcgctg
    11041 gggcaatggc aggccgtcgg cctgaacttt tggcatagga attttatatc tttggtgaat
    11101 aatcaacagg ggtatgattc tagcactgat attcacggtg gatgtcgcag aattgtaact
    11161 aaccggcaaa agatggattg cgaccaccct ggtgcgaaat tataataaaa aacagttctg
    11221 atttttataa aacactcgca atgaggtgat aaatggctaa cgcagatctg gataaacagc
    11281 ctgattctgt atcttccgtg ctaaaagttt ttggcatttt gcaggcgctg ggtgaagagc
    11341 gcgaaatagg gataaccgag ctgtcgcagc gcgtcatgat gtcaaaaagc accgtttatc
    11401 gctttttaca gaccatgaaa accttaggtt atgtggcgca ggaaggggag tcggagaaat
    11461 attccctgac cctgaaattg tttgaactgg gcgctcgcgc gttacaaaac gtcgatttaa
    11521 ttcgtagcgc agatatccag atgcgtgaga tctcccgcct gaccaaagaa actatccacc
    11581 tcggcgcact ggacgaagac agtattgttt acattcacaa aattgactct atgtacaatt
    11641 tgcgcatgta ttcacggatt gggcgtcgta atccgctgta cagcaccgcg attggtaagg
    11701 tactgctggc atggcgcgat ctcgatgaag tgaagcaaat tcttgagggc gtggagtata
    11761 aacgcagtac cgagcggacc atcaccagta cagaagcgtt attacccgtt ctggaccagg
    11821 tgcgcgagca ggggtatggc gaagataacg aagagcagga agaagggctg cgatgcattg
    11881 cggtaccggt atttgatcgc tttggcgtgg tcattgccgg tttgagcatc tccttcccga
    11941 cgttgcgttt ctctgaagag cgtttacagg aatatgtcgc aatgttgcat accgcagcgc
    12001 gcaaaatttc tgcccaaatg ggttatcacg actatccgtt ctgatgagag taagaacctg
    12061 tcggaatatc aaacagacag gttctttatt tagcatgaga aaaataaagt tgaaggtgcg
    12121 ttatattaaa cgcgcttgct ataagaatat tttactcatg agtgagaatc tggttgttta
    12181 ttacccttaa ccattatcga ccacgatatt gcttttacgt aacagtgggc aatctgttat
    12241 ccccaagaaa ccacttttag tgtgcaagta ttgtaccgtg ctggtgcctc tggcagtcag
    12301 ataggtacat tgcaaaccta atcctgcggc attctctttg cttccaatca aaacgccata
    12361 tccgctgagt aataatccta tccataccag tgctatcagc ataactgtgc gaatgatgaa
    12421 tcgcattaca acctcttctc tttttatgtt cgcttaatcg tagcggcaat atgcgctgaa
    12481 gcaagctact cattccgaaa aagcacgaat atcgacatag ttaggcgctg tttaactaac
    12541 gcatgctagt ttaatgacat aaggtaggtg aagcggagat tggagtgaaa aagtttcgat
    12601 gggtcgttct ggttgtcgtg gtgttggctt gcttgctgct ttgggcgcag gtattcaaca
    12661 tgatgtgcga tcaggatgta caatttttca gcggaatttg tgccattaac cagtttatcc
    12721 cgtggtgata tcattttttc agcactagat tttcttcctc tccagcagtg gtagaatagc
    12781 gcctctactc agaggtggtg aaatgaacga agttgtaaat tcaggcgtga tgaacattgc
    12841 gtctttggtt gtatcggtgg tggttcttct tatcgggctc atcttgtggt tttttatcaa
    12901 tcgtgccagt tcacggacta acgaacagat tgaactgctt gaggcgttgc tggatcagca
    12961 aaaacgtcaa aatgcactgt tacgtcgttt gtgcgaagca aacgaaccag agaaagcaga
    13021 taaaaagacc attgagagtc aaaaatcggt tgatgacgaa gatattattc gcctggtcgc
    13081 cgaacgataa tccttcctca atcttgagta accccgtcgt tgaagggtta ctcaattcat
    13141 cctgattata cgcactgtca tggtgcaact gcttcaaatt aaaactctgg tctgacttca
    13201 aataattatc atattattta tttgcgtaac gggatatcag aaaaagatat tttcctcatt
    13261 aatttattag gatgtttaca tcggatttgt gattaagcgt ggtattattt attagcgaaa
    13321 cgtttctctc ttgatttttt tgctcattca tcaatttttc ttattttaaa ttttcaatcc
    13381 ctttggggat tgacttctct ttagggtaat tgatagccgt taaccgactg ttttatgaga
    13441 aaaagtgata taacttttta ttcattgcat agcaaaaaat gtgatattgc acgcactatg
    13501 taataacttc tcccactggc ctggaacaac tgaacttatt gaactatgtt agaaaatacg
    13561 ccagtttaag tatctgcctg aactggcaag gttaagcaca atgatatatc ggcgcgtatt
    13621 ccgttgcata agtgtgcaaa aaaagtggaa gacgtatcga gatttgtgcg tctgatcgag
    13681 acatgtttaa aaatggcttg ccataattaa cgttgtatgt gataacagat ttcgggttaa
    13741 acgaggtaca gttctgttta tgtgtggcat tttcagtaaa gaagtcctga gtaaacacgt
    13801 tgacgttgaa taccgcttct ctgccgagcc ttatattggt gcctcatgca gtaatgtgtc
    13861 agttttatct atgttatgcc tgcgggcgaa gaaaacaatc taaggaattt ttcaaatggc
    13921 aaagattaaa ggtcaggtta agtggttcaa cgagtctaaa ggttttggct tcattactcc
    13981 ggctgatggc agcaaagacg tgttcgtaca cttctccgct atccagggta atggcttcaa
    14041 aactctggct gaaggtcaga acgttgagtt cgaaattcag gacggccaga aaggtccggc
    14101 agctgttaac gtaacagcta tctgatcgaa tccactgatc tgaagtgtga atacgcttca
    14161 atctcgctat aaagcctcgt cgaatgcgag gctttttact atgctttatc ttcgctcctg
    14221 gcgttcggat atttgcccgc cgcgtgattc gcgttacact tgcggccttt agtatcctgc
    14281 cggagttgtc atgtcttttt cctgtccact ttgccatcag cctctttcgc gtgaaaaaaa
    14341 cagctatatc tgtccccagc gacatcagtt tgatatggcg aaagaagggt atgtcaatct
    14401 gctgtccgtt cagcataaac ggtctcgtga tccgggcgac agcgcggaaa tgatgcaagc
    14461 acgccgcgca ttcttagatg ccggacatta tcagccgctg cgtgatgcaa ttgtcgccca
    14521 actgagggaa cggcttgatg ataaggccac ggcggtgctg gatattggct gtggtgaagg
    14581 gtattacaca cacgcatttg ccgatgcgtt gcccgaaatc accacgtttg gtctggatgt
    14641 ttcgaaggta gcgataaaag cggcggcgaa acgctatccg caggtcactt tttgtgtcgc
    14701 ttccagccac cgtttgccgt tttccgatac cagtatggac gccataatac gtatttacgc
    14761 gccgtgtaaa gcagaagaat tagcacgagt agtgaagccc ggcggctggg tcattactgc
    14821 cacgccggga ccgcgacatt tgatggagct gaaggggctg atttacaatg aagtacatct
    14881 tcatgcacct catgcagaac aactggaagg ttttacatta cagcagagtg cggagttgtg
    14941 ttatccgatg cgtcttcgcg gtgatgaagc cgtcgcatta ttgcagatga cgccgtttac
    15001 ctggcgtgcg aagccagaag tctggcaaac actggcagca aaagaagtgt tcgactgcca
    15061 gacggacttt aatattcacc tctggcagcg ttcttattaa ccgtggaagt gcgtccagag
    15121 gatctggacg ccgatgccga tcagtaccag cccgccgaga atttccgctt ttttcccaat
    15181 aattgagccg ataaagcgac caaccatcat ccctaatgtt gacataatca aggttgcaca
    15241 accaatggcc aatgcggtcg cgataatgtt gacctgcagg aaagcaagac caacacccac
    15301 agccatggca tccaggctgg tggcaatcgc ggtggttacc agtagccaga aaccgtgtcg
    15361 acggcgcggc tcttcatctt catcatctgc gccacgaaaa ccctcaataa tcattcgccc
    15421 gccgaggaat atcagcagca caaacgcaat ccagtggttc cattcaagga caaaccggct
    15481 ggctaacatg cccattcccc agccgatcag cggcgtcagg gtttcgacgg caccaaaaat
    15541 aaggccggtt cgcaatgctt cagaaaattt cggtttatgg agggtggcac ctttaccgat
    15601 tgatgcagca aatgcatcca tcgacatacc aaacgcaaga agaacagtag cagtgatatt
    15661 catgacaatg tcctgaccgg ggtatccata aaacacatca tcgcccccag caaacatgca
    15721 tacatgaaca acaagccgtg atggttttcg cgttcatgca ggcatagcga ttgatgtgca
    15781 tatggtctcg cctgactgaa agtattcagt ccgtacgtgc cacgttttgc aacgagtatg
    15841 ttgacacgta catttccgga atctggaaat cggctactcc ccaaatgagg gcgcaacctt
    15901 aacatatttt gagaatataa aacaactgtt ggaatatatt atttcattgc tttattgata
    15961 acgattttca tttggattta tatatcaatg aaacgttaaa ataaataaca aataagggaa
    16021 gaggtaatat agcctaagct atgtctgatt attaaaaata cactaaacat agctttggct
    16081 aaattcataa cttattgagt tgaaacgaga agcttataaa ttttttccag atcgtcgata
    16141 tttcgaacgc ggattaacag ccgacgctgt tctaattgca tcaccaacac gccatcttcc
    16201 gacaagttca tcgctttgat tcggctatat tcaatccaga cattggcgaa gaaaaaacct
    16261 ttttgtttaa agatgatctt cggaacgcgg atccagaata tataaaaacc catcagagcc
    16321 agtgcgctta ataaccatgt cgttattaac gcaccatgat tcgtgacgtt gttatagata
    16381 agaatgacaa tcaatccgac gaagataacg ctatcgatgc gaccacgccg gagcaaagga
    16441 attgccagca gggtggggcc gttacggcgg ggcatgatga actgatcgta gatcgcgaag
    16501 gccaggagtg cggcgatgaa aagaatcagt accaggtccg tgattgtcat taatcctcca
    16561 gataaaaaaa cggggccaaa aggccccggt agtgtacaac agtcttacag tcccagcagg
    16621 ccgcaagcgt aaccagcgat accgatgacg aagaagccaa cgatgatcca cagcgggtta
    16681 acttttttgc gcagtagcca catacaagca aaggtcagca gcagtggtac caggcctggc
    16741 attaactggt ccagaatagt ctggacagta gtaacgtgtt ctttgcccgt ctggtcagta
    16801 atgcgagaga caaccagcgg gatgttgaca tgtgtccact tgttaaccaa tgcccccatg
    16861 acaaacaggc cgaggataga cgccccttcc gtcagttttt gcaggaagcc accacccata
    16921 tctttaacga tatcgatacc tttggagtaa ccatacgcta cgccgtagta acgggttgcc
    16981 agacgcacca ggttaaacag gatgaagaac agcagcggac ctaacaggct gccgctcatc
    17041 gcgataccgg cacccagtgc tgcaaatacc ggacgtacgg ttccccagaa gatcgggtcg
    17101 cctacaccag ccagcggccc catcaaaccg actttgatac cgttgatagc accgtcgtcg
    17161 atctctgcgc cattagcacg ctgttcttcc agcgccaggg ttacgccgag aatcggcgca
    17221 gccacgaacg gctgggtgtt aaagaactcc aggtgacggc gaatagcttg tttacgagct
    17281 tcgttgttct cagggtagag gcgacgaatt gccggtacca tagagaagca gaaacccagt
    17341 gcctgcatac gttcgaagtt ccatgaaccc tggaagaggt tagaacgcag gaagacgcca
    17401 cgaatatcac tttgagtgag ttttttctcg gtggtagttt gagttgtatc aaccatttcg
    17461 ctcacctgtt agtccagttc gttatcgaga tcgttgttac cagctgcctg agcaggcgca
    17521 ccggctacgc ggttgtattt cgggctaagt tggatgtaga gcactgccat aacagtacca
    17581 atcacaccca gagcaaccag gttaaagttg gtgaatgctg cggttacgaa gccgaggtag
    17641 aagaacggca tcaggtagcc agcacgcatc atgttgataa ccatcgcata accaaccacc
    17701 acgatcatgc caccggcgat attcagacca ttggtcacca cttccggaat cgcattcagc
    17761 atgttctgta cttcgctggt accaacagac agcgcaacga tgacggccgg aatagccaca
    17821 cgcattgctt gcaggaacag agaagaaacg tggatccagg aaatcgctgt caggttgccg
    17881 ttatcagcag ccttatccgc agcgtgctgg aaagcaacgg taatagtacg aacgatgatg
    17941 gtcagtacct ggcccgcagc ggccagaggg attgccagtg cgatacctgc accaatgctc
    18001 tgatgacctg cgataaccag aatggtagaa atgatagaag ccagagcggc gtcaggcgca
    18061 actgcagcac cgatgttcat ccagcccagc gcgatcattt ccagcgtacc accgataata
    18121 ataccggttt tcatatcccc aagaacgata cccaccaggg tacacgcgat tagcggacgg
    18181 tgaaactgaa attcatcgag gattgatccc atacctgcga tacaggctac gataaatacc
    18241 agcacaattt gaagagtggt aatctccatt gtacttctcc tgtttacgta agacttaagt
    18301 gtgaaaactg agtgataatc aacacaatac gttacttatc gattttgctg atcagatcca
    18361 tcattttcag tttcggatcg gtggaaacct tacggacttc cagctcaata ccgcgcgcat
    18421 tcagtttctt gaacgcctcg atatcttttt catcaaccga aaccgcgtta ttcacctggg
    18481 ttttaccctg acggaatgcc ataccaccga cgttaacaga ggtgattttc acgccgcctt
    18541 caacgagacg ctctacatct gttgggttgg taaataacag cattacgcgt tcgccagcat
    18601 atttcgggtt gttgtagacg cgaatcattt tggcaacatc aactacgtgt gctgttacgc
    18661 ccggaggtgc aacctgggtg agcagtgtct tacgaacggt atccgcagcc acttcatcac
    18721 taacaacaat aatacgggag acattggttt ctttggtcca gcgggtggcg acctgaccgt
    18781 gaatcagacg gtcgtcgata cgcgcaaggc caataaccat gtagtcgttt ggccccattg
    18841 gttttgccgg agttggagcc gcttttggtg ctgcggcagc gggtgctggc gcggcttttt
    18901 caaccggttt ggctttcagt gctttcacgc cttcacggcc tgtttctact gccagcgcaa
    18961 ccagttcatc aaagcttggg tcatcatcac gagccattaa cgtttccacg agcattggaa
    19021 tgttaacgcc tgcaatgact tcataatgct ctttgtcgac gacaatgcgg ctggcagcat
    19081 tgaacgggct gcctccccat gtatcaacga gaaacagcac gcctttagtg gtgtcgagtt
    19141 ttgccaactg agcgttgtac ttttcaatca gcgtttcggc attttcacct ggaacgaaat
    19201 cgatccagcc gacgttttcc tgctcgccta acagcatttc tgccgtttta agcaactgct
    19261 ctgcagccca accatgtgtg cctataacaa tagcaatggt cacttgctac ctcctttatt
    19321 atcgttaaca cctcaacgtg ccagatgtat ttttgaatcg tcgtccacaa tcgaatcgat
    19381 tcagataagg gctacaacca aaaacccctg cgtttcgcga tttattttag atatcgaaaa
    19441 aattatttta tgtgatgaag atccgtaatt taactttcga ttagcagaaa tttcgaaagg
    19501 taaaatatcc ttgtcacatt cgtttgcaaa ggaaggtaaa tctttgccaa atcagaggcg
    19561 tctctgatat gtttaactcc cgtttaacaa ccatggagta tagggcagta gcccgcagta
    19621 tggatcgtca ccgacgtcat ttcagcatca ggccttttaa cgcctgcctt tctggcactc
    19681 tatgccgcac ctttcgtttg cattttgtcg ttacgcctgc attatttctg gcgtcgaata
    19741 gctattcctt aagcaggagc ttgtcatgga attcttaatg gacccctcaa tttgggcggg
    19801 gctactcacg cttgttgttc tcgaaattgt gctgggtatc gataacctgg tcttcatcgc
    19861 cattcttgct gacaaactgc cgccaaaaca acgcgataaa gcgcgtttgc tggggttatc
    19921 actggcgctg attatgcgtc tggggctgct gtcgctgatt tcatggatgg tcacgctgac
    19981 caaaccgcta tttaccgtca tggatttctc cttctccgga cgcgacctga ttatgttgtt
    20041 cggggggata ttcttgctgt tcaaagcaac aaccgaactg catgaacggc tggaaaaccg
    20101 cgatcatgat tccggccacg gtaaaggcta cgccagtttc tgggtggtcg tcacacagat
    20161 tgtcatcctt gacgccgtct tctcgttgga tgcggtaatt actgcagtag ggatggttaa
    20221 ccatctgccg gtgatgatgg cggcggtagt gattgcgatg gcggttatgt tgctggcatc
    20281 caaaccgctg acgcgattcg ttaaccagca ccccacggtg gtggtgctct gtctgagctt
    20341 cctgttaatg attggtctga gtctggtggc agaaggtttc ggtttccaca ttccgaaagg
    20401 ttacctgtat gccgcgattg gcttctcgat catcatcgaa gtgtttaacc agattgcgcg
    20461 tcgcaacttt attcgccacc agtcgacttt gccgctgcga gcgcgtactg ccgatgccat
    20521 cctgcgtttg atgggcggga aacgtcaggc caatgttcag cacgatgccg ataacccgat
    20581 gccgatcccg gaaggtgcat ttgccgaaga agaacgttac atgattaacg gcgtactgac
    20641 gcttgcgtcg cgttctctgc gcgggatcat gacgccgcgc ggtgaaataa gctgggttga
    20701 cgctaatctc ggggtcgatg aaatccgcga gcaactgctc tcttcaccgc acagtctgtt
    20761 cccggtatgt cgcggtgaac tggatgaaat catcggtatt gtacgtgcta aagaactgct
    20821 ggtggcgctg gaagagggcg ttgatgtggc ggcgattgct tcggcgtctc cggcgattat
    20881 cgtcccggaa accctcgatc cgatcaacct gttgggcgtg ctgcgtcgtg ctcgcgggag
    20941 ctttgttatc gtgaccaacg agtttggtgt ggtacaaggt ctggtcacgc cgctggatgt
    21001 gctggaagcc attgcgggtg aattcccgga cgctgacgaa acgccggaaa tcattactga
    21061 tggtgacggc tggctggtaa aaggcggtac agatttgcat gccttgcagc aggcgcttga
    21121 tgttgagcac cttgccgatg acgatgatat cgcgacggtc gcgggcctcg tgatctcggc
    21181 aaatggtcac attccccgtg tgggcgatgt gattgatgta gggccactgc atatcaccat
    21241 cattgaagcc aatgattatc gtgttgatct ggttcgcatt gttaaagagc aaccggcgca
    21301 cgatgaagat gagtaagcat taacgtaacg gcataatggg cgtgatatgt ccattatgcc
    21361 gggcgggcgg cggttggctg cccgccagcc atttgggaaa atcacgtagc ggcatcggct
    21421 gtgcgtacaa atacccttgc aaaatatgca cgccatgacg gcgcaaatac ttcgcctgtt
    21481 cttgcgtttc cacgccctcc gccaccagtt caatattcag tctttgcccc agcgcgatga
    21541 tgatatcggt caccgtcgag ttaaccgcgt cagaacctat agctgcggta aatgacttat
    21601 caattttcag catgtcagga cgtaatgttt caagccagga aaatgaactg ttgccggtgc
    21661 cgaagtcatc aatcgccagt ttgacgtttt tacgatgtag ctcgcgggca atccgataat
    21721 caacatccag taaggcatcg cgttcggtga tttcgaggat cagttgctga atcgggtgag
    21781 cgctaaacca gtactgattg agatctttta tcagcacacc acggcgaaaa tggctgggtg
    21841 cgacgttaat gccaacatga aactgactac tcatcgggaa aacatggcgc tgacgaatgg
    21901 tttctgccat cacatagcgg gtcagtggca caattaaatg atgttcttcc gcgataggaa
    21961 taaacacatc cggtgaaatc cagccctgac gcggattgtt ccagcgcagc aaaatctcta
    22021 caccaataca ttgctggctg cgcgcattaa gcaaaggctg acagaacaat tcgaactcat
    22081 gttgcgccag gcccagattg atttcgcggg aaaagctcat ccggtaagcg gtcgccagcc
    22141 aggcgatgta gcccaccagt aaacttagca gcaccgccag cggtaattgt gttggaagat
    22201 agtgccatgc cagcgccgta gcccccggac cattaacgtt aatggtaaaa ggaaagcgtt
    22261 gcgaagaaac ctgatagttt tcattgtctt caggttgcgg aagggaatct accagcccat
    22321 taccatagag caaatgccgt ttgtccaccg tcaggctggc gctactgatt tgcggcagtt
    22381 gtggctcaag gagcattgcc gtcagtaagt cgatgttaat catctccatg accccagcat
    22441 tgctactgcc cgctgcaggc gtccattgaa tcaaaaccgg actgccttta atcagggcac
    22501 ggtcgatcgt caggcgtaaa agtggttgcg gtgcaggaag ttcagccaga atgtccacga
    22561 cgggcacatt gcgataacca aaaatgctgg agcaatagag tgttccgtct tgcaccaggc
    22621 caatggatcg cacagtttgg agttttgccg cctgtttacg taatggcaaa tgggcgacag
    22681 agcagggcag accaatcagc ggaagcaaga cttcgctacc tgcctgtagg ggaagcagta
    22741 ctttatctaa ttcctctaca gcgtgattgg cgaattgtac tacccgttgt tgatttaagt
    22801 tacgctgtga aataaatcgc acgctcaggg tcgaagcgag cgtaacgagt gcgcaaatcg
    22861 tacaaacaat cattcgatta cggcgatagg ttttaatgat ccgttgtgct ttttgcatgc
    22921 gggccaccct gtaagcgtat ggcgacaaac gcctgacgca acagtggaag tgtagtgggg
    22981 aatattacag cagacgagaa agcgggtata aattcgccca tccgttgcag atgggcgagt
    23041 aagaagtatt agtcacactg gactttgatt gccagaccac cgcgtgaggt ttcgcggtat
    23101 ttggcgttca tgtccttacc agtttcgtac atcgtttcga tgaccttatc cagcgagacg
    23161 cgcggtgcac tggtgcggcg cagagccatc cgtgcggcgt taatcgcctt cacagaggca
    23221 atggcattac gctcaatgca cggcacctga acctgccctg caaccgggtc gcaggttaaa
    23281 ccaaggttgt gttccatgcc aatttccgcc gccacgcaaa cctgttccgg gctaccgccc
    23341 agcagttctg caagacccgc agcagccatt gaacaggcaa cacccacttc gccctggcaa
    23401 ccaacttccg caccggaaat agaggcgttc attttataca atgcaccaat cgcgcccgct
    23461 gccataaagt aacgggtata gatgtccggg ctgaccgatt caataaagtg gtcatagtaa
    23521 gccagcactg ccggaacgat accgcaggca ccgttggttg gcgcagttac cacacgacca
    23581 ccggcggcgt tttcttcgtt aactgccagc gcaaacatgt ttacccagtc aatgacattc
    23641 atcggatcgt tagacagttt atcgctggaa actaacatcc ggcgcagggc agacgcacga
    23701 cgtggcacgc gcagcgggcc tggcagtaca ccttcggtgt tcatcccgcg atcgatacat
    23761 gcctgcatgg tttgccagac atgcgcgaaa tactcgtcga tctctttctt gctgtgcagc
    23821 gccagttcgt tctgcatagc gagaccagac agcgaatagc cggtttcatt acagtacgcg
    23881 agcagttcgg tggcagattt gaacggatac ggcacgctca cttcgttggc agcatcctga
    23941 ccaaagtgtt cttcatcgac gataaaaccg ccgccgatgg aataataagt tttgctgtag
    24001 acgacttcat cgccgttata ggcgtggatt tgcataccgt tttcatgcag cggcaggttg
    24061 ccgttatgaa aacgcatccc gttgtcgcgc gggaaatcca cttcatgccg tccctgtgcc
    24121 agcagcagac gttcgcgctc ttctacgtcg cgaataaaac cgggaatact gtcgatatcc
    24181 acggtggcag gttcgttacc tgcaagaccc ataataatgg cgatatcggt gtggtggcct
    24241 ttacccgtca gcgacagtga accataaacg tccacggcaa cgcgagtaac gctatccagt
    24301 aagccttttt cgaccagatc atcgacgaac tgtttacctg ccttcatagg ccctacggta
    24361 tgggaagatg agggaccaat ccccacctta aacatgtcga atagactaat cacgataata
    24421 ctcctgacaa ggtgactgga cttccagtaa cgaactaata acagcgcata gtgtaagagg
    24481 gaacgcggcg actggcttaa ctattcacat gaattaaact aatcaaaaac cgcgatttaa
    24541 ctaatattgc gatgattgtc tcaagtagtg ttttgtagat gtaaagataa gtatagtcag
    24601 ggtttcacac caatttgcag cgccagctca cgaattatgc ctgcggtcat tccccataca
    24661 aaatactgtt cgtaccagga cagccatacc cgatgtgaat cgccacggcg gtagatatct
    24721 aaagggtgat aacgacccag atgtaatgcc tgggcgagcg gcatttcaaa caccgccgag
    24781 acttcatctt cactggcgcg atacggcaga tcgggcggga taatgccgac cactggggtt
    24841 acctgatagc cagttacgct atcgacgggc ggcagcacgc cgataacttc aacggcggaa
    24901 ggcggtatag cgacctcttc ttcagcttcg cgcagcgcgg cggcgataac tgatgcgtcc
    24961 gtgtcatcga ctgcacctcc agggaatgcc acttgtccag cgtgtttacg cagatgaatt
    25021 gaacgctgag tcagcaacaa ccccggttgc ggtcgacgga cgatggggat taacacggca
    25081 gcctgacgat gatttagggt ttcccggtta atttgcgggc gcaaaagttg aaagcgcgat
    25141 aaaaaatcat caagcatcag gctacggtat tccacgtctt acttctccag ttggcgtaat
    25201 atcttattaa ctttatcaaa agtttcctga tattccgctt cttcctggct atcggcgaca
    25261 attccaccgc ccgcagagca gtatatttgt ccgttaatgg cagtcagagt gcggatagta
    25321 atgctggtat ccatgttgcc gcaaaagctc aaatagccaa tgctgccgca ccaggcatta
    25381 cgtcgctgcg gttccagttc gtcgataatt tccatagccc gtactttcgg agccccggtt
    25441 attgagccac caggaaaagc tgcgcgcagc agatcgctgg cgtgtaactg ttctggtagt
    25501 tgtgccgtta tggtgctgac cagatgatgc acggcaggga agggttccac cacgaacagc
    25561 tctggtactt ttaccgaacc tgctacggca acacgaccga tatcattacg cattaaatcg
    25621 acaatcatca gattttcggc acgatctttc gctgagttcg ccagtttttc tgcttgtttg
    25681 ctatcttcct gaggatcggg caggcgtggt agcgtgcctt taatagggcg ggtctggatt
    25741 tcactattat cacaaagaat aaaccgctct ggcgaaaggc ttaaaattgc accctgttca
    25801 agacgtaaaa aagcgctaaa tggcgcgcgg ttggcctgat taagctgaag gaatgcctgc
    25861 cattcatcgc cagaataggt cgcatgaaaa cgctgggcga gatttacctg atagcaatca
    25921 ccgctgtgca gatattcctg tacctggcga aatttttcgc cgtactgctc gcgggtcata
    25981 ttggattgcc agtcggaagt aagcgtgaaa tcttcctgcg gcgagaattg ctggctttcc
    26041 agccaggccc gacgggcatt gacatcatta tgactcagca aagaaactgt atgacgctgg
    26101 tggtcgacaa tgagcgccca atcgtagata cccactgcca tatccggcag aacgatatct
    26161 tgttccgcaa tttctggcag tgactcaaaa cggcggccca gatcgtagcc aaacaacccc
    26221 agtgcgccgc cctgaaatgg caaatcttcg ttatgcgttg ggcgaatgtc tgcgcgatcc
    26281 agcacctgct ggagcacctg tagcgggtca tcagtggtcg ttgtgcgttt ttcgctttca
    26341 ctaacaacgg tttctttacc taaagtggtt aaagtgcaaa tcggatcggc gaccacaata
    26401 tcaaagcggc tatacggatg atcggcatag ccggagtgta aaagcatcgc ccacggcagg
    26461 tggcttaagc gggagaaata aaattcagcg gcgtcctgac gccagggtaa agtaatcaca
    26521 gcgggagata acgtcttcat cagtcctgac tctactggct atgtgccggg tagcatgaaa
    26581 taattagcgc caacaattta gcaggagtta acaatgtttg caggtttacc ttcactcacc
    26641 catgaacagc agcaaaaagc tgtcgagcgg atccaggaac tgatggcaca agggatgagc
    26701 agcggccagg caatcgcgtt ggtggcagaa gagctgcgtg ccaaccattc cggcgagcga
    26761 atcgtggcgc gttttgagga tgaagacgag taacacacta atccgtattg atttcgttat
    26821 acaagccgat gattatttga ttgtgagaaa aaggacgctt tttatatgtg attaaaagcg
    26881 tcctgatcca tttatgctga tgtaaatttt actcagacca gtgcttttag ttcttcctgc
    26941 atatatgttg aaaagtattc aggattttta atcacagagc gttcacctga ctccattttt
    27001 tttgcccatc caaccatttt ttccgtaaac ccagggttcc agccttcctg gtcagctcgc
    27061 gccgtaatat cagcggcact cgcaggtact cccagttctt ttaaatattt aaagattccc
    27121 ttcgccgtgc tttcattcat cgaacttggt gttggcgctg atacatccat ggcaccaatg
    27181 aaatccaggg ctttatctat tacagcaggc atattcttat ccttaaaatt aaccatgtta
    27241 tcgataaggc tatatatgac ctta
//
LOCUS       DAJVQG010000041        26883 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJVQG010000041 DAJVQG010000000
VERSION     DAJVQG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26883
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.041"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            34..663
                     /locus_tag="OQM83_001582"
     CDS             34..663
                     /locus_tag="OQM83_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCS1519868.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(664..1824)
                     /gene="ybdL"
                     /locus_tag="OQM83_001583"
     CDS             complement(664..1824)
                     /gene="ybdL"
                     /locus_tag="OQM83_001583"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCS1519869.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1933..3021
                     /gene="hcxA"
                     /locus_tag="OQM83_001584"
     CDS             1933..3021
                     /gene="hcxA"
                     /locus_tag="OQM83_001584"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCS1519870.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGDLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(3031..3228)
                     /locus_tag="OQM83_001585"
     CDS             complement(3031..3228)
                     /locus_tag="OQM83_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCS1519871.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3410..5515)
                     /gene="cstA"
                     /locus_tag="OQM83_001586"
     CDS             complement(3410..5515)
                     /gene="cstA"
                     /locus_tag="OQM83_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCS1519872.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5696..6109)
                     /gene="entH"
                     /locus_tag="OQM83_001587"
     CDS             complement(5696..6109)
                     /gene="entH"
                     /locus_tag="OQM83_001587"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCS1519873.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPMSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(6112..6858)
                     /gene="entA"
                     /locus_tag="OQM83_001588"
     CDS             complement(6112..6858)
                     /gene="entA"
                     /locus_tag="OQM83_001588"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCS1519874.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6858..7715)
                     /gene="entB"
                     /locus_tag="OQM83_001589"
     CDS             complement(6858..7715)
                     /gene="entB"
                     /locus_tag="OQM83_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCS1519875.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7729..9339)
                     /gene="entE"
                     /locus_tag="OQM83_001590"
     CDS             complement(7729..9339)
                     /gene="entE"
                     /locus_tag="OQM83_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCS1519876.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9349..10524)
                     /gene="entC"
                     /locus_tag="OQM83_001591"
     CDS             complement(9349..10524)
                     /gene="entC"
                     /locus_tag="OQM83_001591"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HCS1519877.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            10899..11864
                     /gene="fepB"
                     /locus_tag="OQM83_001592"
     CDS             10899..11864
                     /gene="fepB"
                     /locus_tag="OQM83_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCS1519878.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(11945..13195)
                     /gene="entS"
                     /locus_tag="OQM83_001593"
     CDS             complement(11945..13195)
                     /gene="entS"
                     /locus_tag="OQM83_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCS1519879.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            13306..14310
                     /gene="fepD"
                     /locus_tag="OQM83_001594"
     CDS             13306..14310
                     /gene="fepD"
                     /locus_tag="OQM83_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCS1519880.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            14307..15299
                     /gene="fepG"
                     /locus_tag="OQM83_001595"
     CDS             14307..15299
                     /gene="fepG"
                     /locus_tag="OQM83_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCS1519881.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            15296..16111
                     /gene="fepC"
                     /locus_tag="OQM83_001596"
     CDS             15296..16111
                     /gene="fepC"
                     /locus_tag="OQM83_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS1519882.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(16108..17241)
                     /gene="fepE"
                     /locus_tag="OQM83_001597"
     CDS             complement(16108..17241)
                     /gene="fepE"
                     /locus_tag="OQM83_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCS1519883.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(17457..21338)
                     /gene="entF"
                     /locus_tag="OQM83_001598"
     CDS             complement(17457..21338)
                     /gene="entF"
                     /locus_tag="OQM83_001598"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCS1519884.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(21335..21553)
                     /gene="ybdZ"
                     /locus_tag="OQM83_001599"
     CDS             complement(21335..21553)
                     /gene="ybdZ"
                     /locus_tag="OQM83_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCS1519885.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(21556..22758)
                     /gene="fes"
                     /locus_tag="OQM83_001600"
     CDS             complement(21556..22758)
                     /gene="fes"
                     /locus_tag="OQM83_001600"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCS1519886.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            23001..25241
                     /gene="fepA"
                     /locus_tag="OQM83_001601"
     CDS             23001..25241
                     /gene="fepA"
                     /locus_tag="OQM83_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCS1519887.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            25416..26036
                     /gene="entD"
                     /locus_tag="OQM83_001602"
     CDS             25416..26036
                     /gene="entD"
                     /locus_tag="OQM83_001602"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCS1519888.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(26158..26310)
                     /gene="hokE"
                     /locus_tag="OQM83_001603"
     CDS             complement(26158..26310)
                     /gene="hokE"
                     /locus_tag="OQM83_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCS1519889.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(26661..26813)
                     /gene="hokE"
                     /locus_tag="OQM83_001604"
     CDS             complement(26661..26813)
                     /gene="hokE"
                     /locus_tag="OQM83_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCS1519890.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
BASE COUNT         6414 a         7561 c         7006 g         5902 t
ORIGIN      
        1 ttataacgaa cggataaaag gaaaacgtca ggaatggggg atactatgca acaacgatta
       61 acgcaggatt taacgcaatt tctcgccagc ttgccggaag atgatcgcat caaagcgatt
      121 aatgaaatcc gcatggcgat ccatcaggtt agcccctttc gtgaagagcc agtggattgc
      181 gttctgtggg ttaaaaacag ccaacttatg cctaatgatt acaacccaaa caacgtggca
      241 ccacccgaaa aaaaactgct gcaaaaatcc attgaaatag atggttttac ccaacccatt
      301 gtggtcacac atacggataa aaatgccatg gaaatcgtgg atggttttca ccgccatgaa
      361 attggtaaag gctcatcatc attaaaatta cgcttaaaag gttatttgcc ggtgacctgt
      421 ctggaaggta cgcgtaatca acgcatcgcc gcgactattc gtcataatcg cgctcgtgga
      481 cgccatcaaa tcaccgccat gtcggaaatc gtccgtgaac ttagccagtt gggatgggac
      541 gataataaaa ttggcaaaga gctgggaatg gacagcgacg aagtgctgcg cctgaagcaa
      601 attaacggcc tgcaagagct gtttgccgac cgtcaatatt cccgcgcctg gacggttaaa
      661 taactaaagc tggcgcaggc gttcagctgc tgccagcaac gtcgattcct tcttggcaaa
      721 acagagacga atcagtttat gtgggaaggg atcggcgcaa aacaccgaca gcggaatcgc
      781 cgctacgccg tgctcctgcg tcagccactg gcaaaactca acatcatcca gggtagaaac
      841 cgcgctgtaa tccaccagca aaaagtatgt accttcacac ggtaaaatct ccagccggct
      901 ttcatttaag gcatttacca gaatatcgcg cttctggcga taaaagtccg gtaacgcaag
      961 ataatgctca ggttctgcac gtagcatatc agcaagcgcc agctgtgccg gggtattcac
     1021 cgaaaaggtc agatactgat gtaccttgcg aatttcggcg ctgatgggcg ctggcgcaac
     1081 acaataaccc actttccagc cggtcatatg ataggtcttg ccaaatgaag aaaccgccac
     1141 tgcccgctca cgcagctgcg aatgcgccag cacactggca tggccctgtt gtgaaaagtt
     1201 gatgtgctcg tagacttcat cgctaatgac aaaaatctcg tgcccggcga tcgcctgcca
     1261 caaagcggcg aaatcagcct gctgccagac agttgcactg gagttatgcg gagtgttgag
     1321 gatcaccagt ctggtgcgct cgcttaataa tgcggcaaat tcctgccagt caacgcgaaa
     1381 atgcggtggt tgcagtgcca tacgcttcac tattccccca gaaagcgcga tggcgggggc
     1441 gtaactgtca tagctgggat caaaacaaat cacttcatcg ccattgcgca ccagtgcggt
     1501 aatcgccgcg tataacgctt ccgtcgcccc tgccgttacg gtgatatcgc tatcggcatc
     1561 tggttgatag ccatacaaac gttccgtttt ctgagcaatc gcctcgcgca aggcctgcac
     1621 gccggtcatg ggcgcgtatt ggtttgcccc ctgggcaacg tggtacgcca gccgctcctg
     1681 taaatagcgc ggaccatcaa aatcaggaaa gccttgcgac aggttaatcg cctggtgttg
     1741 ctgcgccagc gcgctcatct gggtgaaaat agtggtgcca agttgtggaa gtttgctttg
     1801 tggaatcaga gggttatttg tcatttttat tgtacctgac gaagttgcgt gttgaaggca
     1861 ctataacacg atgctagtat ttggcaatca agacgtttag atgtctaaat aaaacaataa
     1921 ggacaacaca acatgcctca caatcctatc cgcgtggtcg tcggcccggc taactacttt
     1981 tcacatccag gaagtttcaa tcacctgaac gattttttca ctgatgaaca actttctcgc
     2041 gcggtgtgga tctacggcaa acgcgccatt gctgcggcgc aaaccaaact tccgccagcg
     2101 tttggacttc caggggcaaa gcatattttg tttcgtggtc attgcagcga aagcgatgta
     2161 cagcaactgg cggctgagtc cggtgacgat cgcagcgtgg tgattggcgt cggcggcggc
     2221 gcactgctcg acaccgcgaa agccctcgcc cgccgtctcg gtctgccatt tgttgccgtt
     2281 ccgacgatcg ccgctacctg cgccgcctgg acaccgctct ccgtctggta taacgatgcc
     2341 ggacaggcgc tgcattatga gattttcgac gacgccaatt ttatggtgct ggtggaacca
     2401 gagattatcc tcaacgcgcc gcaacaatat ctgctggcgg ggatcggtga cacgctggcg
     2461 aaatggtatg aagcggtggt gctggctccg caaccagaaa cgttgccgct aaccgtgcga
     2521 ctggggatca ataatgcgca ggccattcgc gacgtattgt taaacagtag cgaacaggcg
     2581 ctgagcgatc agcaaaatca acagttaacg caatcatttt gcgatgtggt ggatgctatt
     2641 attgctggtg gtgggatggt cggtgatctg ggcgatcgtt ttacgcgtgt ggcggcggct
     2701 catgccgtgc ataacggtct gactgtcctg ccgcaaaccg agaaatttct ccacggcacc
     2761 aaagtcgctt atggcattct ggtacaaagc gccttgctag gtcaggatga tgtgctggcg
     2821 caattaaccg gggcgtatca gcgttttcat ctgccgacca cgctggcgga gctggaaatg
     2881 gatatcaata atcatgcgga gatcgacaaa gtgattaccc acaccctgcg tccggtggaa
     2941 tacattcatt acctgccagt cacgctgaca ccagatacgt tgcgtgcagc gttcgaaaaa
     3001 gtggaatcgt ttaaagcctg acgtgcaaat ttagcagcag cgcgcgccgc cttttccacc
     3061 gtagcgcgcg tcctgccgct cacggaaaaa ctcttcgtag gtcatcggcg tttgatcggg
     3121 atggttaacc cgcatatgtt cgacatagtt gtcgtaatca ggcataccaa tcatcagctt
     3181 cgccgcctga cctaaatatt ttccggcttt tgccagtgaa tcaaacatct tggcctccgc
     3241 taaccatgtt gctgaaagag tgccggatgc ggcgtgaacg ccttatccgg cctacaaatg
     3301 ctatcaacgc gctcggtcag tcccctcgcc ccatcgggga gagggttagg gtgaggagga
     3361 accctcggca ctatgccaac attcgccaac atcccccctc actctgactt tagtgtgcgc
     3421 cttttgcctg cgccacgatc tcctcgacat tttccggcat tggctcatac ggcgtttctt
     3481 tcgccgttgg cttcggatct ttcaatgccg ccagtgccga cttaatcgag aacagcgcca
     3541 gaaccaccac gaccaccata aagaagatgg ttaacccggc atccagacgg ttgttgaaca
     3601 ccagttgcgc cagctgtgac tcagtatact gcgacggaat attgccgctg tctatcattg
     3661 cctggaactt attagcaatg gccaggaagc cgactttcgc atccgggcta aacgctttct
     3721 gccagcctgc ggtcagggta caaatcagca gccaggccgt tggtaccagc gccacccagg
     3781 cgtaacgttg acgtttcatc ttgaacaaca ccacggcaca gagcatcagc gccatccctg
     3841 ccagcatctg gttggcaata ccaaacagcg gccacagagt gttaatgccg cccagcggat
     3901 cgaccacgcc ctgatggagg aagtagcccc acgccagtac gcacagcgct gttgccagca
     3961 ggttagcagg cagtgaatcg gtccgcttca ggccaggaga caccacgccc agcagatcct
     4021 gcaacataaa gcgcgcagca cgcgtacccg catctaccgc cgtcagaata aacagagctt
     4081 caaacagaat ggcgaagtga taccagaacg ccacatccat cataccaccc agcgcaccgt
     4141 gcaggatgta agccatcccc accgccagcg tcggcgcacc gcccgcacgg gaaatgatcg
     4201 actgttcacc cacttcgctg gcaatctggt tgagcgtatc cggagtaatc gcaaagcccc
     4261 agctactcac cacctgcgcg gcagaagcga ccacatccgc cgtccctgcc ggagccagca
     4321 ccgccatcgg gctgttcatg gcgaagtaca cgcccggatc gatgatacag gcggaaacca
     4381 gtgccataat cgccacgaag gattccatta acattccacc gtagccgata aagcacgcct
     4441 gcccttcgtt cgccagcatc ttcggcgtgg tgccggaaga gatcagcgca tggaagcctg
     4501 acaccgcgcc acaggcgatg gtgataaaca ggaacgggaa cagattaccg gtccataccg
     4561 ggccagtgcc atcgacaaat ttggtcagcg caggcatggt cagcgtcgga cgcataatca
     4621 aaatgcctac cgccagacca acgatagtcc cgattttcag gaaggtagag aggtagtcac
     4681 gcggggccag cagtaaccac accggcagca ccgccgccac aaaaccgtag cccaccagca
     4741 tccaggtcag ctgcacgccg gtaaagtcaa agtacggtgc ccaggtcggg ctttctgcca
     4801 cccagccgcc agagataatg gcaaaaataa ggaacaccag gccaatcact gacacttcgc
     4861 caatacgccc cggacgcaga tagcgcaggt agattcccat aaaaatcgcc agcggaatgg
     4921 tgaacgcaac ggtatacgtt ccccacgggc tatgagtcag ggctttcacc acgatcatcg
     4981 ccagtactgc aaggataatg accatgatca taaagcaggc taccagcgca atcaccccgg
     5041 cagttggccc catctcttct ttgaccagct cacccagcga gcgaccgtca cggcgcgtag
     5101 aaacaaacag caccatgaaa tcctgcaccg caccggcgag aaccaccccg gcgagcagcc
     5161 agatcatccc cggcaggtag cccatttgcg ctgccagcac cggccccacc agcggccctg
     5221 ctccggcaat ggccgcaaaa tggtgaccga acagcacttt cttgtccgtc ggcacatagt
     5281 ccagcccgtc gttatggcgc accgctggcg tcatacgcgt cgggtcaacc gccagcacat
     5341 ttttggcgat atacagccca taaaaacggt aagcgatcag atagatacag accgacgcca
     5401 ccacaatcca cagcgcgttg atctgttccc cacgatttaa agcaatgtat cccagagcaa
     5461 atgctcccat tacagagagc actgtccaga cgaggtattt ccctgatttg ttcatagttg
     5521 ttatccgtgt gcgtgtccat agagatgtta cattttgttt ctataacatc tttgtacgat
     5581 ttaacaacac ggtaaccatg taaatcggag ttgaaaccaa tatttaactt aacaatgtta
     5641 actcgatcac tccgttaacc gagttttcct gcaatctcat ttaattctgt cgggctcatc
     5701 ccaaaactgc cgtacccagc cgacaagtgc agcaacgccg cccctgttca tcgaaaacga
     5761 tgatttccca gctctgattt tgccgcccaa gatgcagcgg ctggcagacg ccgcgtacct
     5821 tgccttcaga catcgggcga tgatgggtgg cgttaagttc cgtacccacc acacactgcc
     5881 cgtcacgggt catcataaat ccggccatcg atcccagcgt ttcagccagc gccgccgacg
     5941 cgccgccatg cagcaggcca aacggctgat gagtacgggt atcaaccggc atttcggctt
     6001 ccagcacatc atctcccaga cgggtataca caattcccag atgcgccacc attgtgttat
     6061 cgctggtggc gttcagttcg tcgagcgtta aatggcgttt ccagatcatg cttatgcccc
     6121 cagcgttgag ccgccatcga ccacaatatc ctgtagggtg atatggctgg cgaggtcaga
     6181 ggcgaggaac aaaatcgtgt tggcgatctc ttgtggacgg gcgattttcc ccagcggaat
     6241 gccgagttta aactgctcgc caaagccgcg aatacgctgt tcttcggcgt catcgctcac
     6301 ccacagcgtg cgttgcatat cggtgtcggt ggagccaggc gaaaccacat tacagcgcac
     6361 gccgctaacc gccagttcca gcccgacgct caacgccagg cttttcagcg ccgctttcga
     6421 tgcgccataa gcactcatac caatacgcgg cgtgtgtgcg gcgtcggacg ccacagtgac
     6481 aatcgccccg ccccgctgac ggcgaaactg gttcatggtt tgctgaaaca ggttaaacgc
     6541 accgccgacg ttaaccgcaa aagtctgctg ccagtcctct ttactgagct gatcggtcgc
     6601 gcccatgcgc aaaattcccg ccgcattgac cagcacgtcc agtcgctccg tttcagctaa
     6661 cagtcgctga catacctgcg cgacctgcgc agcatcggca acatccatca cttcggtcgc
     6721 aaagggatat tgctcctgag cgaacgcttg atcaaaaccg gtaactttcg ctcccgcttc
     6781 aacaaacgcc agcgccgtgg cgtagccgat acctttacct gcgccggtta cccagacatt
     6841 tttaccgctg aaatccatta tttcacctcg cgggagagta gcttccacca ggcgtcgatg
     6901 gtcgggtttt tcgccagcat gacaaagtcg atatcgccat gcactttgcg ccagcgcgcc
     6961 gccagcgcca tcatgcgcac cgaatccaga ccgtagtcga tcaggttgtc gtcatcgaac
     7021 ggttcatcgg actcgtccag caacggcagg ataacctcac gcagcgccgc tttcctggcg
     7081 gggacaggtg ctggcagtaa tccttcagtc atcaccaccc ggccagaccg cccggccaca
     7141 tatttcagcg acatcaaatg ctcgtcacgg ctgaaatcgg ccagcgcatc cgccaccata
     7201 aacggtttaa tatcgcgcat aaatgcgtcg gttgcggtgg tcatacagcc aatgtgggca
     7261 tataccccgg taataatcag ctggttacgt ccactctctt tgagcatttg ctccagcgga
     7321 gaacgatgaa acgcgctgta gcgccacttc accagcaccg tgtcgtcggc atctggcgtc
     7381 aggcgatcca ccaccttttg ctgttccggc gaacgggtca ggcccggccc ccacatatca
     7441 ttcaacagcg cccgatcttc atcgctctgc tctttcggct gggcggtgta ataaaccggg
     7501 atattgtgct gtttgcagta gtcgcgcagc gcagcaatat tcgcgatcac ctgctccatc
     7561 atcgggcagt tctcgcccca gaagctgaca aaatagtcct gcatatcatg gattaacaac
     7621 gcggcacgtt gcggttcaaa ggcccagtca actttattct gcggaatatc gtgagactcc
     7681 ggcagtgcgt aagcctgtaa ttttggaata gccatcgtgt tctctccttc aggctgatgc
     7741 gcgtgacgcc agccactgac gtaattgttt tttatcgact ttcccgaccg ccgtcagcgg
     7801 aagtgaatcc acacactcca cgcgatccgg taatttaaat tcggcaatac cctgttcacg
     7861 caggaaacga cgcacctgca ccgcgcgcag cggctctttt accaccagat aagcacagct
     7921 tttttcgccc atcagctcat cttccatgct caccagtgcg gcatagatca ccgccggatg
     7981 gcgcagcagc aggttttcga tctcttcggc agcgatcttc tcgccgccac ggttgatctg
     8041 atctttctcg cgcccctgca cggtgatgta accctctgga tcaatagaga tcagatcgcc
     8101 ggaacagtaa aaaccgttgg catcaaaggc actggcattg tgctgcggac ttttgtaata
     8161 accgcggaag gtgtacggcc cgcgcgtcat caggcgtcca acttcccctt gcggcagtgg
     8221 atttccttca gcatcagcaa cccatacttc atcgtccgga cacattgggt aaccctgggt
     8281 atggataatt ttctccgcgc tatcatcaag tcgggtgtag ttcaccagcc cttccgccat
     8341 gccaaacacc tgctgcaact ggcagccaat ctcagcggaa atacgcgccg caagggtggc
     8401 agaaagacgt gcgccgccga cctgtaacag tttcagcgag gcaagctgcg cccggctttc
     8461 gccttcagcc agcgcctgca accacaggct aactgccggc ggcaccagcg cggtaacgtt
     8521 aacctgatgt tttgcaatca acgggaagca aagcgtggcg ctgggatcgg ccgccagaac
     8581 aaccgttcct ccggcaagaa agacgcccag cgatcctggc gaactcatag cgtagttatg
     8641 agccgccggg atcgcgcaca ggtagcgtgt ctgttgtgtg aactgacaaa tctcgacgct
     8701 acgacgcacg ctgtagtagt agtcgttatg agtgcgcggg atcagcttcg gtgtaccggt
     8761 ggtgccgccg gaaagctgga aataggccac ttcatcagca ggtgatggcg tagcagtaaa
     8821 accgtctgcc ggatggttaa tcgcatcctg caagttatgc tcaccgctgt cgttgagcag
     8881 ttgcaccacg cgaatggagg aatgttctgt gacgaaagta ttgaggaaat catccccgct
     8941 aaacagcgca tgttggcgat cggcaatcag taacgccggt tcaatctggc tggcataggc
     9001 gttcagttca ctacgctgat ggctgaacaa cgccagcacc ggcgcaacgc ccagtttcag
     9061 cagtgcgaaa aaggtaatgt aaagttcagc gacgttaccc agttgtacca gcgcggtttc
     9121 accgggttta atgccctgac ggcgtaaact acacgcgagg ttatccgccg cctgattcag
     9181 ctcccggtaa ctcaactgtc gctcgccgtc gataaccgcg atgctgtcac tcgcagcgtg
     9241 gcgagtcaga atgtcggtca gcggcaaatc ctgccagtag cctttttccc gatagcgacg
     9301 ggcaaactct tccggccagc gggtgaatgg aatgctcatc ctcgctcctt aatgcaatcc
     9361 aaaaacgttc aacatggtag aaagtttgac gcctgtttcg cgccactcac ccaacggtga
     9421 cgacgcaggc acaatccccg ctccggcaaa cagacgcacc tgattttccc gcagcttcgc
     9481 gcagcggatg gtcaccaccc attcgccgtt accttcgctg tcacaccaac ccacaatgcc
     9541 gccaaacagt tcgcggtcaa acggctccag ttcagcaata acctgggtcg cggcctgatg
     9601 cgggaaaccg ctcagcgcgg gggtcggatg cagcagacag gccagagtca gtgcgttttc
     9661 ttgcgaattc gctttacctt caaagggagt tgcgagatgc cacagcgtcg gcgtggtaat
     9721 caattgtgga gaggagggaa cgtgtaactc actactgcgt tcgcgcagta cctctttcat
     9781 cgcctgagtc accagttcat gttcatggcg atctttttct gacgccagca gacgattacc
     9841 cgcttcgcga tcgagcactt catccggctg acgacgcgcg gaaccggcta acggaatgga
     9901 gctaaaacgc tcgccgtctt tacgtagcag cagttccggg ctggccccca gcaggacgcc
     9961 accatcagcc agcgggacat ggaagttgta actaaccggg ttttgcgcaa tcaaccgttc
    10021 cagcaatacg ccactatcaa tggcggcgtc agtggtgata tcaatcaatc gtgacaacac
    10081 cactttgtcg acctgcggcg tggcggtaag tgcggcggcg cgggcaacca tctgttcaaa
    10141 cgtggtttgt tcaggaattg cctggcgttc caccacgttc agcgactggc tgcgggtaaa
    10201 acagtgggct gaggtctgtt tttcctgacg ggagaacgac tgccaggatt cgggaatata
    10261 cagcgacgaa ggctgacgtg gatcgaaggg aatcgccccg accatcaccg gatttttgat
    10321 gccctgcgct ttggcatcgg caaacagcgc ggcgagtttt tgctggaagg gactgtcggg
    10381 cgaatccccg ttcacagccg gttcatcgaa gcgggcgaaa catcctgacg tcgtaaaact
    10441 gcggtacggc gacataaaga aaaagcgatt gggcgcaagt gttgccatgg tctgctgtac
    10501 ttcctcagcc agtgacgtat ccatatcatc ctccacaaaa tgataaaggc tttaataatg
    10561 attatcattt atattttcgg tcgctaacct aaaagcaaac gcgcactatg tcaactcttg
    10621 aggtaacgca atctgcaact cggggttgca tctggttgtg gttacaatga aaatgagaag
    10681 cattattgat gaattcgcat aagcgcaatg tgatgtcctg cgccgttttg ccccctctcc
    10741 cttccagggt gagggctggg gtgagggtta atgttcgcac cagtgctggc tgttcccctc
    10801 accctaaccc tctccccaaa ggggcgaggg gacggattgt gcgctttgtc gaatttgtca
    10861 ttacgccctt aaccttatta ataacaggaa gctgacttgt gagactcgcc ccgctctacc
    10921 gcaacgccct tctattaaca ggacttttgc tttcaggaat agccgtagtt caggccgccg
    10981 actggccgcg tcagattact gacagccggg gcactcatac cctggaaagc cagccgcagc
    11041 gtattgtttc caccagcgtc accctgaccg gatcactgct ggcgattgat gctccggtga
    11101 tcgccagcgg cgcgaccacg ccgaataacc gcgtcgcaga tgaccagggc tttttacgcc
    11161 agtggagcaa ggtggcgaaa gaacgcaaac tgcaacggct ctatatcggc gaaccgagcg
    11221 ccgaagccgt tgccgcgcaa atgccggatc tgattttaat tagcgcaacc ggcggggatt
    11281 cggcgctggc gctgtatgat cagctttcca ccatcgcccc gacattaatc atcaattacg
    11341 acgacaaaag ctggcagtcg ctgttaacgc agcttggcga aattaccggg catgagaaac
    11401 aagcggcaga gcggattgca cagtttgata agcaactggc agcggcgaaa gagcaaatca
    11461 aattaccgcc gcagccggtc actgccattg tctataccgc cgctgcacac agtgccaatc
    11521 tctggacgcc ggaatcagca caagggcaga tgctggaaca actcggcttt acgctggcga
    11581 agttgcccgc agggttaaac gccagccaaa gccagggcaa acgccatgac atcattcagc
    11641 ttggtgggga aaatctggct gctgggttaa acggtgaatc gctattcctg ttcgccggtg
    11701 atcagaaaga cgccgatgcg atttatgcca atccactgct cgcgcacctg cctgcagtac
    11761 aaaacaagca ggtttatacg ctgggaaccg agacgttccg cctggattac tacagcgcca
    11821 tgcaagtgct ggacagactt aattcactgt taaaaaaaga ttgataaaac atacaattag
    11881 agcactaata atcccctcgc ccctttgggg agagggttag ggtgagggga aaatgcacct
    11941 ttagctaact gtcagacgct gtcacctgcg gcggcgtctg gcgaaaacgt cgcaactcca
    12001 ccagcaccag caataacaac acgccgataa tcaacaaacc aaaaccgctc gcgcttgcgg
    12061 aagcaaccgg tgtcatcatc gcgcccaaac cacccaacag cgccgcgcct atggcatcgc
    12121 ccgtcacgtt ctgcgccgtc cacaaaccgt taatccgccc taacatcgct tccggggttt
    12181 gcgtttgcag cattgtatat tgcagcaacg aactaaccgc gctcagccag ccgaacagcg
    12241 ccagacaaac cacgcctaaa atccacatcg gcatcaggcc aaacagacca atggcgagga
    12301 acgatcccag cgtggagagc agcatcaata accctggtcg cgcactatgt gccagcttcc
    12361 cgctggttaa cgcaccaata gccgcaccga gcgggatcgc cgcatagaga aaaccaatct
    12421 gtgccgccga catctgccag ttgtcagcca gcgccggata cagtacccgc accgcgctcg
    12481 ccatcgtcaa taaaccaccc agcaacgcaa tccctcctac cagcgggctg gcgagcagaa
    12541 aacgaaatcc tgccagtaat gatttcaacg gatgctcgcg cggctgcggt ggcggtggca
    12601 acgccggaag gcttaacaac ggtagcaagg taataaacgt gcccgccgcc gccagcccgt
    12661 agttccaggc tacgccgccg gtcgccagca ataaaccgcc aatcatgggc gaaatcaccg
    12721 accccagacg cacggtcaac atggtgatcg ccccggcctg cattaagttt tcacgcccta
    12781 ccagtgcagg tgttgccgcc agtagcgccg taacaccaag tgatgcgaaa aaaccatccc
    12841 ataaaccaag taaatagatt gccagcaatg acggctccgg cagcagcgcg ttcaggcaca
    12901 gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa aatcactttt ttgcgctcat
    12961 agcgatccgc cagcacaccg ccaaccatca ggccaacaaa catcgcgccg ccggtcagcg
    13021 tcaccgaaag ccccacctgc caggtagaat gcgtcatcat ctggatctgc accggcaccg
    13081 cgacgccgag caaacccaga gacacaattg agataaaacg agcgaggaat actgcgcgaa
    13141 acgccgggtg cgttttcaac aggctgaggt taagcagcca ggattgttta ttcattacaa
    13201 tgccttgcca tcaaatattt taaataccta ttcttaggct gcacatgcta acatatccaa
    13261 ataagatcga taacgataat taatttcatt atcatggaag ttcgtatgtc tggttctgtt
    13321 gccgtgacac gcgccattgc cgtgcccgga ttgctgttat tactgattat cgcgacggca
    13381 ttaagcctgc tcattggggc aaaatcactc cccgcgtcag tagtgctgga ggccttctca
    13441 ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg cgcgactgcc gcgcaccctt
    13501 gccggtttac ttgcaggcgg tgcgcttggg cttgctgggg cgttaatgca aaccctcacc
    13561 cgaaatccac ttgccgaccc cggcttgctt ggcgtgaacg ccggagccag ctttgccatt
    13621 gtgctgggtg cggcgctgtt tggttactca tccgcgcagg aacaactggc gatggccttc
    13681 gccggggcgc tggtggcctc attgattgtt gcctttatcg gcagtcaggg cggcgggcag
    13741 ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg cggcggtgct ggaagggctg
    13801 accagcggca tcgccctgct taatcctgac gtctacgatc aactgcgttt ctggcaagcc
    13861 ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg tgctgatccc ggttctgatc
    13921 gccggagcaa ctgcgctatt actgagtcgc gcgctgaaca gtttgagcct cggcagtgac
    13981 accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt tgattggtct gctggcgatt
    14041 accgtgcttt gtggtagtgc gacggcggta gttggcccga ttgcctttat cggcctgatg
    14101 atgccgcaca tggcgcgctg gctggtaggt gccgatcatc gctggtcgct gcccgtcacg
    14161 ctacttgcta cccctgccct gctgctgttt gccgatatca tcgggcgggt gattgtgccc
    14221 ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg caccggtgct gatcttcctc
    14281 gtgcgacgta aaacgcgagg tggtgcatga tttacgtctc tcgccgatta ctcatcacct
    14341 gtttgctgct gatttccgcc tgtgtggttg caggtatctg gggattacgc agcggtgccg
    14401 tcacgctgga aatctcgcag gtattcgccg cgttgatggg cgatgcgccg cgcagtatga
    14461 cgatggtggt caccgaatgg cgtttaccac gcgtgctgat ggcgctgttg attggcgcgg
    14521 cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg taacccgctc ggcagccctg
    14581 acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct ggtggcgatg gtgctgtttg
    14641 gtcaggacct gacggctatc gcgctggcag caatggtggg cggcattgtc acttctctgt
    14701 tggtctggct gctcgcctgg cgtaacggta tcgacacctt tcggttgatt attatcggca
    14761 tcggcgttcg cgccatgctg gtggccttta atacttggct gttgctgaaa gcgtctttag
    14821 aaacggcgct aacagcgggt ttgtggaatg ccggatcgct caatggcctg acgtgggcaa
    14881 aaacttcgcc ttccgcaccc atcatcatat tgatgctcat tgccgccgcc ttgctggtgc
    14941 ggcgaatgcg cttgctggag atgggcgatg ataccgcctg cgcgctgggc gtcagcgtcg
    15001 agcgttcgcg tctgttaatg atgctggttg cagtggtgct taccgctgcg gcaacggcgc
    15061 ttgccgggcc gatttccttt attgctttag tcgcgccgca cattgcccga cgcattagcg
    15121 gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg agcgctgtta ctgctggcgg
    15181 ccgatctctg cgcccaacaa ctgtttatgc cgtatcaact tccggttggc gtcgttaccg
    15241 tcagcctcgg cggtatttac cttatcgtct tgttaattca ggagtctcgc aaaaaatgac
    15301 cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg ggatatggca aatataccgt
    15361 tgcggaaaat ctgactgtag aaattcctga tggtcacttc acggcaatta tcggaccaaa
    15421 tggctgtggt aaatccacgt tactgcgtac cttaagccgc ctgatgacgc ctgctcacgg
    15481 gcatgtctgg ctggatggcg agcacattca acattacgcc agtaaagaag ttgcacgccg
    15541 gattggtctg ttggcgcaaa atgctaccac gccgggcgat atcaccgtgc aggagctggt
    15601 ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc tggcgcaaag aggatgaaga
    15661 agcggtaacg aaagcgatgc aggccacgag aataactcat ctggcagatc aaagcgtgga
    15721 taccctttct ggcggacaac gccagcgagc gtggatcgcg atggtgctag cccaggaaac
    15781 ggcaattatg ctgctcgacg aacccacgac ctggctggat atcagtcatc agattgatct
    15841 actggaactg ttaagcgaac tgaaccgcga gaaaggctat accctggcgg cggtgctgca
    15901 cgatcttaat caggcctgtc gttacgccag ccatttgatt gcattgcggg aagggaaaat
    15961 tgttgctcag ggagcgccga aggagattgt cactgctgaa ctgattgagc gcatttatgg
    16021 tctgcgatgc atgatcattg acgatccggt agccggaacg ccgcttgtgg tgccgctcgg
    16081 acgaacggca ccttcaaccg caaatagtta aactaagtgg tctgccatca tggcatcctg
    16141 ttttctggat gccatcgcat agcgcaataa cacgctacca caagccacca tcccgccgat
    16201 cagcgcggaa aggatcacaa taatcgcctt acccggaccg tcttttttca ctggcaatga
    16261 cgggcttaac tgatatttaa acggcgtaaa attcacatcg ttgacatttg cttttgttaa
    16321 ttgctcgaca agatactgcc gattacgtaa ttcaccgttc agttccgcaa cgtcagtaac
    16381 cgctttttca atttccagtt tgcgttcaat accgtctgcg ccgagagaaa tagagaaatc
    16441 ggggtcatct ttaacggcct gaccattact gtatacgggc tttttaattc ctgccgcgtt
    16501 ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca tcaagttgat ttttcgtttt
    16561 aatgcgatcc tgagccagtt tttctttttc aaactgggtt ttgatctcca gtttattacg
    16621 gacgttttct atcgactctt tcaccaccaa cgcagagata taatcgatat acccgctcaa
    16681 aacggtctgc gcctctttac tggttggcgc ggtaaaactt agcgtccagg aggtatacag
    16741 tgacggttca tcttttttct tactggcatt gtcatcaacc gctttcattt tttcgctcaa
    16801 tgcaacaata gcgcgatgca aatccagttc atcgattttc gcctctttta attggtccat
    16861 cacataaggt gatgaacgca ggtactcttc cagcaagcta acagactgaa acttcttgat
    16921 aaacaggtta aatgcaccgc cacggtcgat attaacatcg agatcaagaa cgcggagttt
    16981 ggtaaaggtt ttctccaggt cctgccactg aatggcttcg gcgggagtaa taacggctga
    17041 gctggtccat ttttgcggca ggatgaaaga gatcagtaaa cctgcgcagg caaatgcaaa
    17101 aacgaccgcc atgaccgttt ttttggcccg ccataaaact tcgattagat taagtaaatc
    17161 aatttcatta ttactgggcg acgccagagg atactcagaa aaatgagcgt cacttccctg
    17221 tttaatattc aatgatgaca tggggtataa cctgaaaatg agtccaatag gggaaaatct
    17281 ccacggcgct cactttacca gttggtgaga attatccgaa gctgaagttt gtaaattcct
    17341 tccactgaat gcaatattca ctctatttaa gagaaatatt catcaaacct ataaatcaaa
    17401 caaataaaaa acccataatt attttctgta attatgggtt tatagataat attaatttac
    17461 ctgtttagcg ttgcgcgaat aatcggccca atttttacaa acgcccctgg agagataata
    17521 tccacatgcg cacaatcctg acgatagata tccagctccg caatccacgg cgaccaggcg
    17581 cgttcaggac tcataccttc ctgaagcgtg cgttcagcaa caaacaacgt cgctttaccg
    17641 tcaaacggta cgctatgagc agtcgtcagc aggcgcacag catcagcgta gttgccttca
    17701 atggtggtaa acaactccgt tgaagtactt ccctgctgtg ccgccaggaa ggcctcgcgc
    17761 tcgcggttaa tctccgccag cacttccggg tccagaccat tagcttcttt ttcctgccag
    17821 ttttgcgttt ctggcggcca ggtatccagc aagccaagaa atgccacctg ttcgccacgg
    17881 gcacgcagtc gcgccgcaat accctgcgcc agcgtaccgc caagggaata ccccagcagg
    17941 taataagggc cgtgcggttg ttgctcaagt aacgttgcca gatgcgcttc gcagacttca
    18001 tccaggtttg tcgctgtctg catgggtcca tgagggcgcg gcgactgaat gccaataatc
    18061 gaccattgtg gatcgagata acgcgagagt acgctaaact gccaggcaaa accggacgcc
    18121 ggatggaaac aaaacagcgt cgggccatta ccttcacgca acggcagaat ggtttcgaat
    18181 cccatgcgcc gggagctgtc ctcttcacca tcaataatcg ttgccagttt ggcgacggtt
    18241 gacgcgacca tcacctgccc cggcgtcacc tgacgagcaa actgtcgact taactgcgct
    18301 gccagtttca ttgccagtag cgaatgaccg cccagcgcaa agaaatcagc atcggcatcc
    18361 tgcacgtcac aacccagcaa cgacgcgaat gccgcggcga taatcgtttc actgcccgct
    18421 ttcggtgcac gtcccgacgc ttgtgtcttc agttcaggca acggtaaggc tttgcgatcc
    18481 agcttgccgt tggcgctaag aggtaactgt ggaagttgca gcagaaccac cggtaccata
    18541 tgtggtggca atgtttcacg aagctgcgcc tgcaatgcgc tggtatccaa cggcaggccc
    18601 gattgcgaca ccagatagcc caccaactga cgcgcatcac caccagtggc tgccgcctgg
    18661 ttaatcacac aggcgtgggt aacggcttgt tcgacatccg gcagcgcctg catcacgcga
    18721 tcgatttcgc ccagttcgat acgctgcccg cgaattttta gctgatcgtc actgcgcccg
    18781 aggtactcca ccgcgccgtt atccagccag cgggcaacgt ctccggtacg gtacatccgt
    18841 tcgccaggag caaaaggatc ggcaataaag cggctggcgg tcagatcggg gcgtccgaga
    18901 tagccctgcg ccagttgaat gccggtaaga tagagatcac ccgccacacc cggcggcacc
    18961 ggatgcatca tcgcatcaag aatacgcagc cccatgttcc acaccggata accaatcggc
    19021 acactgctgc cgcgcacctg tgccagttcc tcgccaaaag ccggatacca gctgacatct
    19081 accgccgctt ccgtcgggcc atacagatta tgtaacggcg caccggttaa ctgttgccat
    19141 tcacggcata aatcagccgg taaggcttca ccactacaga aaacctgttt caacgtcgcg
    19201 caactctggc gagcggtttg cggagtcagc gaggcaacaa atgccgccag catcgacggc
    19261 acaaagtgcg tggtcgttac gccatattcg gcaaagaatt gctgcatagc gagcgggtca
    19321 cggtgcgctt ccggttcagc catcaccagt tttgcccctg cgataaacgg ccagaaaaac
    19381 tcccacaccg agacatcaaa actgcacggc gttttttggg caacgacatc ttcgcctgta
    19441 agcgggtagt gattttgcat ccataacagg cggttaacga tagccgtctg cccgaccatt
    19501 acccctttcg gcctgccggt ggagccagag gtaaagatga tataagccgt gtggtgcggt
    19561 tgtgaaagtt gcagcggcgc actgccctgg ggggtaagcg gggcgttata gcaaaggctt
    19621 gttaaattgg gaacatcgct aaagcgcggc agttgatcgt cggtggtaat taacagcgac
    19681 ggacgcgcat cttccagcat cattttcagg cgatcgtccg gatagccggt atccagcggt
    19741 agccaggccg cgccagcttc aactattgca tggagtgcca gggtcaaaaa gaccgagcgc
    19801 ggtagtgcca ccgccacgct gtcccctggt ttaacgccgc gctcacgcag cagattcgcc
    19861 agcgccacca cctgctcgcg catttcccga tagctgaaca ggtaacgcgc atctgccagc
    19921 gccggagcat ccggtgtttt tgccgcttgt tctgccacca gcgcgctcag cgtggtttct
    19981 ggaatctcaa cctgagtggc gttgatctgc gccagctgcg catactcacc tggcagcata
    20041 atatcgacat cgccgcacaa cagcgccgga tccgcggcga actgggcaat cagcattttc
    20101 aggcgttcag catgctggat taacgttggc tcatcgtaac gctgtttatt ggcgaggatc
    20161 tcaatactca aatcaccgtg ttcatccggg aacagggcca gttcaaggtc attaaccgga
    20221 ccggttgcca gggtatgggt ttgcgcctga acaccaggaa tatccagttg gtaatcaaat
    20281 accttaatat tgagtaccgg accaaacagc ggttcatcac ctgccgctcg cccgctgtca
    20341 cggacaattt gttcggcatc gtaacgttga tggcgacgca tttttttcag ttgagccgcc
    20401 agtcgggttg ccagttccgg cagcgtttct tgcgccgcaa tgtgaatacc caacggtaaa
    20461 acgttgagca cgggtccgct agccgtcagc gccgccgagc ccagtcgacg cataaagata
    20521 aatccggcgg catagtccat gcgattgcac aatcgcccca gccacaaggc tgccagcgca
    20581 agggctaaat cggtacgctg cacacctgaa agttgcgtag ccagctggcg gaattccccg
    20641 tcggtaaatt ccagtttcag gcgcagaata tctgccgaag cgctgcgccc cggtaaaggt
    20701 gccggagaaa gtgacgcggg cggcggcagt tgacgacgct gttctgccca gaatgccgca
    20761 tcacgctgcc aggcttcgct ttcgcggtat tgctggtact cttccactac atcagcgaaa
    20821 ggcgtaaacg gcgaagcagg cgttggttcg ccacgcagcc atgtgcagta aatattggcg
    20881 atctggcgag taatggccgg gaaactgaag ccatcgacca gcaaatggtg ataacgctga
    20941 taccagtacc agcggttatc cgcaacatgt atcagctgat gaaagaccag tggtttaccg
    21001 ctatcgacgc gcaaatcttg ttgcaaatcc gccagcatta atgcctgcgc agtaccgtgc
    21061 ggatcaatat tggttcgcag gtcgataatt tctggctgtt cgaatatcag cgcatcatcg
    21121 acccactgcc agacttcgcc gttatcttcc gtaaaacgca tccgcaacgt atctgcttgc
    21181 gctagtccgg caaccaccgc gcgggccagt aatggcgcat caacatctcc agttaactca
    21241 acgtaatgcg ccacgctcca ggcggagggt aaatccgaca gtttttctgc catccagatg
    21301 ccgggctgtg cggcgaccaa aggtaaatgc tggctcattg tgcctcctgc aactgggtga
    21361 aattggccgg tgtcagagta cgccagtgga cttccagcca ctgctggcag gacgcctgtg
    21421 actgcggctg acacacaatg tcccagcctg ccgataagag gcattgttgc ggccacagac
    21481 tgaattgccc ctgcgcattg cgcaaaatgt aaaacgctcc ctgcggatca tcgaagggat
    21541 tactgaatgc catactcaac tcctgtcatg gaaaagtggt tgccagaggt cgattaaccc
    21601 ctgcatcaag ccaccgcgcc aacaaagcgc atcatgtccg ccgtcaacct gacgccagaa
    21661 aatggattct tttatagggt gcaattgcgc atacagcgcc tgattggccc gcatgatcat
    21721 cggctcgcga atacccgctt ccagcacaat gcgcagacct tcggcgctaa cttcaccagc
    21781 ttttagcttt tcaagtaaca cgccctcttg ctgcccgtcc cgatgcggcc accagtacga
    21841 tcctgactgg cttaatacac agccaaagcg ttcaggccag tgcagtccgg catacagcgc
    21901 ggacagccca ccaaaactct gcccggcgac cacggtgcga tcggcgcgat cgctaaaagg
    21961 ggcaatagct ttcaccaggg gtaataactc ttgctgtact gccagccaga aatccgcatt
    22021 acacggcagt tcgtgggcgc ggtgcgtggt gtcgatagcg tcgatcaaca catacacggc
    22081 gggaggaagt tgctgacgat gggtcagcga agtcagcaca ggccagacgg gcatactttg
    22141 cgcccaaaat tcgccatcga gcaaaactgc cagcggacgt tcttctgctg ttacatcgcc
    22201 ggtggtaaaa atccatacac gacgtgaatt tttcaaccgt tcacttttcc agataatttc
    22261 tttggctgct atttctggca cttgcggaca atcccatccc ggttgcagag gcgcttgcgg
    22321 catttcgagt gcagaaacag cgtgccctcg cccgcctttc cagctttgtg gattcagcgg
    22381 atcggctatt gcctggggta atagttttcg ccagccttca cgtaattcga ggcgatcggg
    22441 ggatggtgca gaaaaaatgt catcgcgttc ggtgggaata aagcagtagc tgccgcgcca
    22501 gttggcattg agttgtgtcg tccactgcca gacatcagta cctgcaattc gctgcatcga
    22561 ctggggctgg ctgttctgat gatgatcggt cacaccagtg atgtagatcc atacgcgctt
    22621 tatcgtcgag tattcttcag aaccttgggg atcacgccac cagaaagtga cctcaaacat
    22681 ttcgtcattc agacgctgcc attccgggcc atgtttcgac tgccaccagc tctcacttcc
    22741 tacttttaac gccgtcacac cataacccca tgtttactgt gcaatttttc attgattgca
    22801 gaaatatatt gataatatta ttgataacca tttgcatttg caatagcgta atggcgcgcc
    22861 gtgggaagcg cggacattta ttaaccaact gcactgcgtg tctttcagga tcaaaggttt
    22921 tcgcggtagc gggatgcgtc gtgttgagga cgaccatgcc cgacagttgc aattcgtggc
    22981 aaaaatgcag gaataaaaca atgaacaaga agattcattc cctggccttg ttggtcaatc
    23041 tggggattta tggggtagcg caggcacaag agccgaccga tactcctgtt tcacatgacg
    23101 ataccattgt cgttaccgcc gccgagcaga acttacaggc gcctggcgtt tcgaccatca
    23161 ccgcagatga aatccgcaaa aaccccgttg cccgcgatgt gtcggagatc atccgtacca
    23221 tgccaggcgt taacctgacc ggtaactcca ccagtggtca gcgtgggaat aaccgacaga
    23281 ttgatattcg cggtatgggt ccggaaaaca cgctgatttt gattgacggc aagccggtaa
    23341 gcagccgtaa ctcggtgcgt cagggctggc gtggcgagcg cgatacccgt ggtgatacct
    23401 cctgggtgcc acctgaaatg attgaacgta ttgaagttct gcgtggtccg gcagctgcgc
    23461 gttatggcaa cggcgcggcg ggcggcgtgg ttaacatcat taccaaaaaa ggcagcggtg
    23521 agtggcacgg ctcctgggac gcttatttca atgcaccaga acataaagag gaaggtgcca
    23581 ctaaacgcac caacttcagc ctgaccggtc cgctgggcga cgaattcagc ttccgcttgt
    23641 atggcaacct cgacaaaacc caggctgacg cgtgggatat caaccagggc catcagtccg
    23701 cgcgtgccgg aacgtatgcc acgacgttac cagccgggcg cgaaggggtg atcaacaaag
    23761 atattaatgg cgtggtgcgc tgggatttcg cgccattgca atcgctggaa ctggaagcag
    23821 gttacagccg ccagggcaac ctgtatgcgg gcgacaccca gaacaccaac tccgattcct
    23881 atacccgctc gaaatatggc gatgaaacca accgtctgta tcgccagaac tactcgctga
    23941 cctggaacgg cggctgggat aacggagtga ccaccagcaa ctgggtgcag tatgaacaca
    24001 cccgtaactc gcgtattccg gaaggtctgg caggcggtac cgaagggaaa tttaacgaaa
    24061 aagcgacaca ggatttcgtc gatatcgatc ttgatgacgt gatgctgcac agcgaagtta
    24121 acctgccgat tgatttcctc gttaaccaaa cgctgacgct gggtacggag tggaatcagc
    24181 aacggatgaa ggacttgagt tccaataccc aggcgctgac cggggcgaac accggcggcg
    24241 ctattgatgg tgtgagtgcc accgaccgta gcccgtattc aaaagcagaa attttctcgc
    24301 tgtttgccga aaacaacatg gagctgactg acagcaccat cgtaacgccg gggctgcgtt
    24361 tcgatcatca cagtattgtc ggcaataact ggagcccggc gctgaacata tcgcaaggtt
    24421 taggcgatga cttcacgctc aaaatgggca ttgcccgtgc ttataaagca ccgagcctgt
    24481 accagactaa cccgaactac attctctaca gtaaaggtca gggctgctat gccagcgcgg
    24541 gcggctgcta tcagcaaggt aacgatgacc tgaaagcaga aaccagcatc aacaaagaga
    24601 ttggtctgga gttcaaacgc gacggctggc tggcgggcgt cacctggttc cgtaacgatt
    24661 atcgcaataa gattgaagcg ggctatgtgg ctgtagggca aaacgcagtc ggcaccgatc
    24721 tctatcagtg ggataacgtg ccgaaagcag tggttgaagg tctggaagga tcgttaaacg
    24781 taccagttag cgaaatggtg atgtggacca ataacatcac ttatatgctg aagagtgaaa
    24841 acaaaaccac gggcgaccgt ttgtcgatca tcccggagta cacgttgaac tcaacgctga
    24901 gctggcaggc gcgggaagat ttgtcgatgc aaacgacctt cacctggtac ggtaagcagc
    24961 agccgaaaaa atacaactac aaaggtcagc cagcggttgg cccggaaacc aaagaaatca
    25021 gtccttacag cattgtgggt ctgagcgcga cctgggatgt gacgaagaat gtcagtctga
    25081 ccggcggcgt ggacaacctg ttcgacaaac gtttgtggcg tgcgggtaat gcccagacca
    25141 cgggcgatct ggcaggggcc aactatatcg cgggtgccgg ggcgtatacc tataacgagc
    25201 cgggacgtac gtggtacatg agcgtaaaca ctcacttctg atgctaacgt cagattgttg
    25261 acaaagtgcg cgtcgttcat gccggatgcg gcgtgaacgc cttatccggc ctacaaaaat
    25321 cttgccaatt caatatattg caggaccatg taggcctgat aagcgtagcg catcaggcag
    25381 ttttgcgttt gtcatcagtc tcgaatatgg tcgatatgaa aactacgcat acctccctcc
    25441 cctttgccgg acatacgctg cattttgttg agttcgatcc ggcgaatttt tgtgagcagg
    25501 atttactctg gctgccgcac tacgcacaat tgcaacacgc tggacgtaaa cgtaaaacag
    25561 agcatttagc cggacggatc gctgctgttt atgccttacg ggaatatggc tataaatgtg
    25621 tgcccgcaat cggcgagcta cgccaacctg tctggcctgc ggaggtatac ggcagtatta
    25681 gccactgtgg gactacggca ttagccgtgg tatctcgtca accgattggc attgatatag
    25741 aagaaatttt ttctgtacaa accgcaagag aattgacaga caatattatt acaccagcag
    25801 aacacgagcg actcgcggac tgtggtttag ccttttctct ggcgctgaca ctggcatttt
    25861 ccgccaaaga gagcgcattt aaggcatcgg agagacaaac tgaagcaggt tttctggatt
    25921 atcagataat tagctggaat aaacagcagg tcatcattca tcgtgagaat gagatgtttg
    25981 ctgtgcactg gcagataaaa gaaaaaatag tcataactct gtgccaacac gattaattga
    26041 caacatctgg tacgattcgc ccgcagccat cactgactgc gggcgaaagt gtaaagcagg
    26101 tgccttacca ctctgacctg acaaccggat atgcagggat tgctccccgc acaacggcta
    26161 cttcttcggt tcgtaagcga gaacagcctt aaactcaata ttacgttcct ttacggtgaa
    26221 ctcacacagc gagtctccga ccagaagcgt aaatcccagc accgtcaaac acagcactat
    26281 gactgccaca agggcatatt tcgtcagcat gtttatattg cctccatgcg aagaagggac
    26341 taccatccga gttgttgagg ttcggagtgg cagccccagg attgatagaa atatctcctg
    26401 gggcttcatc tttccagacc tcagagtaaa cctgaaaccg gaaagcttca ggcacccgcc
    26461 gttatcttac ccttcctcag caaaatatct gtttatttat acagtaagtc ctgtttcgga
    26521 cttctgttgg caacactcgt tattgctcgt aaaacgaaat cgcccgcagc catcactgac
    26581 cgcgggcgaa agtgtaaagc aggtgcccta ccatcctgac ctgacaaccg gatatgcggg
    26641 gattactccc cgcacaacgg ctacttcttc ggttcgtaag cgagaacagc cttaaactca
    26701 atattacgtt ccttcaccgt aaactcacac agcgagtctc cgaccagaag cgtaaatccc
    26761 agcaccgtca aacacagcac tatgactgcc gcaagggcat atttcgtcag catgtttata
    26821 ttgcctccat gcgaagaagg gactaccatc caaattgttc gggtttggag tggcagcccc
    26881 agg
//
LOCUS       DAJVQG010000042        26827 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJVQG010000042 DAJVQG010000000
VERSION     DAJVQG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26827
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.042"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..924
                     /locus_tag="OQM83_001605"
     CDS             <1..924
                     /locus_tag="OQM83_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1519891.1"
                     /translation="TSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKL
                     NDSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPV
                     NIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNL
                     TAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQ
                     MRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGT
                     VNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(967..1827)
                     /gene="rsmI"
                     /locus_tag="OQM83_001606"
     CDS             complement(967..1827)
                     /gene="rsmI"
                     /locus_tag="OQM83_001606"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCS1519892.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1891..3927
                     /gene="lpoA"
                     /locus_tag="OQM83_001607"
     CDS             1891..3927
                     /gene="lpoA"
                     /locus_tag="OQM83_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCS1519893.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3885..4280
                     /locus_tag="OQM83_001608"
     CDS             3885..4280
                     /locus_tag="OQM83_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCS1519894.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4300..4890
                     /gene="diaA"
                     /locus_tag="OQM83_001609"
     CDS             4300..4890
                     /gene="diaA"
                     /locus_tag="OQM83_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCS1519895.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4900..5475
                     /gene="yraP"
                     /locus_tag="OQM83_001610"
     CDS             4900..5475
                     /gene="yraP"
                     /locus_tag="OQM83_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCS1519896.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5589..6629)
                     /locus_tag="OQM83_001611"
     CDS             complement(5589..6629)
                     /locus_tag="OQM83_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS1519897.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6702..7337)
                     /locus_tag="OQM83_001612"
     CDS             complement(6702..7337)
                     /locus_tag="OQM83_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCS1519898.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7465..7983
                     /gene="yhbO"
                     /locus_tag="OQM83_001613"
     CDS             7465..7983
                     /gene="yhbO"
                     /locus_tag="OQM83_001613"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCS1519899.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7963..8406)
                     /locus_tag="OQM83_001614"
     CDS             complement(7963..8406)
                     /locus_tag="OQM83_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCS1519900.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8457..8759
                     /gene="yhbQ"
                     /locus_tag="OQM83_001615"
     CDS             8457..8759
                     /gene="yhbQ"
                     /locus_tag="OQM83_001615"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCS1519901.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8746..9249)
                     /locus_tag="OQM83_001616"
     CDS             complement(8746..9249)
                     /locus_tag="OQM83_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1519902.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9243..9767)
                     /locus_tag="OQM83_001617"
     CDS             complement(9243..9767)
                     /locus_tag="OQM83_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCS1519903.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9976..10971
                     /locus_tag="OQM83_001618"
     CDS             9976..10971
                     /locus_tag="OQM83_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCS1519904.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10980..11858
                     /locus_tag="OQM83_001619"
     CDS             10980..11858
                     /locus_tag="OQM83_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCS1519905.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12064..13071
                     /locus_tag="OQM83_001620"
     CDS             12064..13071
                     /locus_tag="OQM83_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HCS1519906.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13189..14433)
                     /gene="mtr"
                     /locus_tag="OQM83_001621"
     CDS             complement(13189..14433)
                     /gene="mtr"
                     /locus_tag="OQM83_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCS1519907.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14587..16476)
                     /gene="deaD"
                     /locus_tag="OQM83_001622"
     CDS             complement(14587..16476)
                     /gene="deaD"
                     /locus_tag="OQM83_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCS1519908.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16469..16549)
                     /gene="yrbN"
                     /locus_tag="OQM83_001623"
     CDS             complement(16469..16549)
                     /gene="yrbN"
                     /locus_tag="OQM83_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCS1519909.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16656..17540)
                     /gene="nlpI"
                     /locus_tag="OQM83_001624"
     CDS             complement(16656..17540)
                     /gene="nlpI"
                     /locus_tag="OQM83_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCS1519910.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17649..19784)
                     /gene="pnp"
                     /locus_tag="OQM83_001625"
     CDS             complement(17649..19784)
                     /gene="pnp"
                     /locus_tag="OQM83_001625"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCS1519911.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20031..20300)
                     /gene="rpsO"
                     /locus_tag="OQM83_001626"
     CDS             complement(20031..20300)
                     /gene="rpsO"
                     /locus_tag="OQM83_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCS1519912.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20449..21393)
                     /gene="truB"
                     /locus_tag="OQM83_001627"
     CDS             complement(20449..21393)
                     /gene="truB"
                     /locus_tag="OQM83_001627"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCS1519913.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21393..21794)
                     /gene="rbfA"
                     /locus_tag="OQM83_001628"
     CDS             complement(21393..21794)
                     /gene="rbfA"
                     /locus_tag="OQM83_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCS1519914.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21958..24630)
                     /gene="infB"
                     /locus_tag="OQM83_001629"
     CDS             complement(21958..24630)
                     /gene="infB"
                     /locus_tag="OQM83_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCS1519915.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24655..26142)
                     /gene="nusA"
                     /locus_tag="OQM83_001630"
     CDS             complement(24655..26142)
                     /gene="nusA"
                     /locus_tag="OQM83_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCS1519916.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26170..26622)
                     /gene="rimP"
                     /locus_tag="OQM83_001631"
     CDS             complement(26170..26622)
                     /gene="rimP"
                     /locus_tag="OQM83_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCS1519917.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
BASE COUNT         6357 a         7449 c         6745 g         6276 t
ORIGIN      
        1 acctctgcaa cgctaagtag cactaactgg caatacgcct gttcctgctc tgcgggtaag
       61 gcagttaaac ttgtctatat ggtcagcccc gtacttacca ccactggaca tcagacagga
      121 tattacaaac tcaatgacag cctggatatt aaaaccacat tacaggcaaa cgacattcca
      181 ggactcacaa ccgaccaggt tgtctctgtt aacacccgat tcacacagat aaaaagcagc
      241 accgtatatt ctgctgcaac ccaaacgggt gtttgccagg gtgacacgtc tcgttatgga
      301 cccgttaata ttggtgcaaa taccaccttt accctgtatg tcaccaagcc atttctcggc
      361 tcgatgacca ttccgaaaac ggatattgcc gtcattaaag gcgcgtgggt cgatggaatg
      421 ggaagcccgt ctacaggtga cttccatgat ttagtcaagt tatcgattca gggaaatctc
      481 accgccccac agtcgtgcaa aattaatcag ggcgatgtta ttaaggttaa ttttggattc
      541 atcaatggtc agaagtttac cacccgcaat gccatgccag acggttttac tccagtagac
      601 tttgatatca cttatgactg tggtgatact tcaaagatta aaaactcgtt gcaaatgcgc
      661 atcgacggta caactggggt agtagaccag tacaacctgg tcgccaggcg aagaagttca
      721 gacaatgtgc ccgatgtcgg tattcgtatt gaaaatctcg gcggcggagt tgcaaatatt
      781 ccttttcaga acggtatcct tcccgttgat ccttccgggc atggcaccgt caatatgcgc
      841 gcctggccag ttaatctggt cggtggtgag ctggaaacag gaaaatttca gggcaccgcc
      901 accattaccg tcatggtgcg gtaaaagaag acgttgcgtt ttgattaacg caacgcttcg
      961 gcactgttac ccctgctgct ccagcgcata cttatacagc gcatttttct tcacgccatg
     1021 aatttctgcg gccagcgccg ccgctttttt cagcggcagt tctgcctgta gcagcgccag
     1081 cgtgcgcagg gcatcggcgg gtaagtcttc ttcctgtgct ttatgacctt cgacaatcag
     1141 caccatttcg cctttgcgac ggttttcatc ttcctttacc cacgccagca gctcgccaac
     1201 gggcgcgccg tgaatggttt cccaggtttt ggtcagctca cgcgccagaa ccacgtagcg
     1261 ggattcgcct aataccgcaa cgatatcttc caggctatct aacagacggt gggtagattc
     1321 ataaaaaatc agcgtgcgcg gctccgcttc aatggctttt agcgcatcac ggcggccttt
     1381 tgatttggca ggtaaaaagc cttcgtaaca gaaacggtca gagggtaaac ccgcggcgct
     1441 taacgcagtg atagcagcac acggtccggg tagcggcacc acacgaatcc ccgcttcacg
     1501 gcaggtacgc accagatggt agccaggatc gttaattagc ggcgttccgg catcggaaac
     1561 cagcgcaatg ttttgcccct cttgcagctt cgccagcagc gtttcggctt tttgttgttc
     1621 gttatggtcg tgcagcgcaa acaaccgggc attaatccca aaatgttgca gcaataaacc
     1681 ggtgtgacga gtatcctcgg cggcaatcag atcaacggcc tgtaatacct ctaacgcacg
     1741 ctgggtgata tccgccagat tgccgattgg cgtcggtaca atgtaaagct ggccctgaga
     1801 attatccgcc gattggtgtt gtttcattgt gtcgtccgta ttgccgattt aatattgagc
     1861 attgcgtaaa aaaatatcac tggatacatt atggtaccct caacattttc tcgtttgaaa
     1921 gccgcgcgtt gtctgcctgt tgttctggca gccctgattt tcgccggttg tggcacccat
     1981 actcccgatc agtccactgc ttatatgcag ggcacggcgc aggctgattc tgccttttat
     2041 cttcagcaga tgcagcaaag ctctgatgat accaggatca actggcaatt actcgccatt
     2101 cgtgcactgg tgaaagaagg taaaaccggg caggcggttg agttgtttaa ccaactaccg
     2161 caagaactga acgattctca gcgtcgcgag aaaacactgc tggcggtaga gattaaactg
     2221 gcgcagaaag attttgctgg cgcgcaaaac ttgctggcga aaatcacacc tgccgattta
     2281 gaacaaaacc agcaagcgcg ttactggcag gcaaaaatcg atgccagcca ggggcgtcct
     2341 tccattgatt tactgcgcgc gttaattgct caggaaccgc tgctcggcgc gaaagaaaaa
     2401 aagcagaata ttgatgccac ctggcaggcg ctctcctcca tgactcagga acaggcgaat
     2461 acgctggtga tcaacgccga cgaaaatatt ctgcaaggct ggctggatct gcagcgcgtc
     2521 tggtttgata accgtaacga tcccgacatg atgaaagccg ggatcgccga ctggcaaaaa
     2581 cgttatccga acaatccggg cgcgaaaatg ctgccaacgc agttggttaa cgtaaaagcg
     2641 tttaaaccag cctcgaccaa caaaatcgcc ctgctgttgc cgctgaatgg ccaggcagcg
     2701 gtatttggtc gcaccattca gcaaggcttt gaagcggcga aaaatatcgg cactcagcca
     2761 gtggcggctc aggtagctgc cgcacctgcc gcagacgtag ctgaacaacc tcagccgcaa
     2821 accgtggatg gcgttgccag cccggcacaa gcctcggtta gcgatctgac cggtgatcag
     2881 cctgcggccc agccggtgcc tgtaagcgcc ccggcgacaa gcaccgcagc ggtaagcgca
     2941 cccgcaaatc catccgcaga gctgaaaatc tacgatacct catcacaacc acttagccag
     3001 atcttaagcc aggttcagca ggatggcgcg agtattgtgg ttggtccgtt gctgaaaaat
     3061 aacgttgaag agttgctgaa gagcaacacc ccgctgaacg tgctggcact gaaccagcca
     3121 gagaatatcg aaaaccgcgt caatatttgt tacttcgcgc tttcacctga agacgaagcg
     3181 cgcgatgcag cgcgtcatat tcgtgaccag ggtaaacaag cgccgctggt gctgatccca
     3241 cgcagttcat tgggcgatcg cgtagccaat gcgtttgcgc aagagtggca gaaactgggc
     3301 ggcggcaccg ttctgcaaca aaaatttggt tccaccagcg aattacgcgc gggtgttaac
     3361 ggcggttctg gtatcgcttt aacgggtacc ccgattactc ccagagcgac aaccgactcc
     3421 ggcatgacga ccaacaatcc aacgctgcaa accacgccaa ccgatgacca gttcaccaat
     3481 aatggcggtc gtgtcgatgc ggtgtacatt gtggcaacgc cgggtgaaat cgcttttatc
     3541 aaaccgatga tcgccatgcg taacggtagc cagagcggtg caatgctgta cgccagctcc
     3601 cgcagcgcac aaggcaccgc aggcccggat ttccgtctgg agatggaagg tttgcagtac
     3661 agcgaaatcc cgatgctggc gggcggtaat ctgccgttaa tgcagcaggc actcagcgcg
     3721 gtgaataacg attattcact ggctcgcatg tatgcgatgg gcgtcgatgc ctggtcgctg
     3781 gcaaatcatt tctcacaaat gcgccaggtt cagggttttg aaatcaacgg taataccgga
     3841 agcctgacgg caaacccgga ttgcgtgatt aacaggaagt tatcatggct acagtaccaa
     3901 caaggtcagg tagtccccgt cagttaacca ccaaacagac cggcgatgcg tgggaagcac
     3961 aagcgcgtcg ctggctggaa ggcaaaggac tgcggtttat cgccgctaac gtgaacgagc
     4021 gtggcggcga gatcgatctg ataatgcgtg aaggccggac caccgttttt gtcgaggtac
     4081 gctatcgccg ctctgcgctt tatggcggcg cggcagccag tgtgacccgc agcaaacaac
     4141 acaaattatt acagactgcc cgcttgtggc tcgcgcgtca taatgggagt tttgatactg
     4201 tggattgccg gttcgatgtg gtagccttca ccgggaatga ggttgagtgg attaaggatg
     4261 cctttaatga ccactcataa ttaaggttta aggattagcg tgcaagaaag aattaaagct
     4321 tgcttcactg aaagcattca aactcaaatt gcggcggcag aggcgcttcc ggatgccatc
     4381 tcccgtgcag ccatgacgct ggttcagtct ctgctcaatg gcaacaaaat cctctgttgt
     4441 ggtaatggaa cttccgctgc caatgcacag cattttgctg ccagcatgat caaccgtttc
     4501 gaaacggagc ggcccagctt acctgccatt gcactaaata ctgataatgt tgtcttaacg
     4561 gcgattgcca acgatcgctt acatgatgaa gtgtatgcaa aacaggtgcg ggcgctgggt
     4621 catgcgggag atgtattgtt agccatttcc acccgtggca acagccgcga tattgttaaa
     4681 gcagttgaag ccgccgttac gcgtgatatg accattgtgg cattgaccgg ctatgacggc
     4741 ggcgagcttg caggtttgtt agggccacag gatgtggaga tccgcattcc ttcgcatcgt
     4801 agtgctcgca ttcaggaaat gcatatgctg acggtaaatt gcctgtgcga tctgatcgat
     4861 aacacgcttt tccctcacca ggatgattaa ggagaataca tgaaggcatt atcgccaatc
     4921 gcagtcctta tttccgcgct gctgttgcaa ggttgtgttg ccgctgccgt agtgggtacc
     4981 gctgctgtgg gtaccaaagc cgcaactgac ccacgcagtg tcggcaccca ggtggacgat
     5041 ggtaccctgg aagtgcgcgt gaacagcgca ttgtcgaaag acgaacagat taagaaagaa
     5101 acccgtatta atgtcacggc gtatcagggc aaagtgctgc tggttgggca gtcaccaaac
     5161 gctgaacttt cggctcgcgc caaacagatt gctatgggcg tagacggtgc caacgaagtg
     5221 tataacgaga ttcgtcaggg ccagccgatt ggtctgggcg aagcatctaa cgatacgtgg
     5281 atcaccacca aagtgcgttc gcagctctta accagcgacc tggtgaaatc gtccaacgtg
     5341 aaagtgacca ccgaaaacgg tgaagtgttc ctgatggggc tggtgactga acgtgaagcg
     5401 aaagcggcgg cagatattgc cagccgggtg agcggcgtaa agcgggtaac cacagcgttt
     5461 acgtttatta aatagcagcc ccttgtaatg cctgatgcga cgcttgccgc gtcttatcag
     5521 gcctacaacg atgcaaaccg taggtcggat aaggcgttta cgccgcatcc gacatgtttt
     5581 ccctcaaatc agaataacag cgccagcccg ccgacaatca cgcctgacac tgccaccatc
     5641 gcccccgtca gccataaggc tttcgccggg aacgctttgc gcagcataat cagtgacggc
     5701 aaactcactg ccgggagcgt catcaacaat gccagcgccg gagcggttcc catacctgcc
     5761 agcatcatcg tttgtacaat cggaatttct gctgccgtgg gaatgacaaa caagcatcct
     5821 gctaccgcca tcgccaccac ccacatcagg ctgttatcga cagcaccatc ggcatggggg
     5881 aataaccaga cgcgtgcggc acccaacacc agtactgcaa ggatgtaaac cgggatcgta
     5941 ctccagaaaa gtgtccatag cgccctgccc cagcggctaa aaaaaccgct ctgtgcttcc
     6001 ggtatgtcaa tttcgaccgg tgcctgcgtt tgcggtgttt cacgcaccca tttttgcacc
     6061 agcgtcgcaa tcagcaacac catcaccagc ccggccacca gacgaatcgc cgcaaaaccc
     6121 cagccgagga caaaccccat aaacaccagc gtcgccgggt ttaacaccgg attgcccatc
     6181 cagaatgcca gcgcaccgcc catcgatacc tgttggcgac gcattcccgc cgcgaccgga
     6241 gccgcacagc aggtacacat catgcccggc agcgaaaaca gcgttcccag cagcgtgccg
     6301 cgaaagcgcg attgtccaag cgtacgcaac aaccagtcac gcgggatcag cacctgaatc
     6361 aacgaaccga ggatcacccc cagcaccgcc gctttccata ccgcgaggaa atagatcatc
     6421 gcgtaatcca acgccgcctg ccacggatta gcatccgcct gcgcaaggat agatttaccg
     6481 atactgtggg tttcggcagc agtaaacgct ttgccgtagt aaggttgcca tttcacatac
     6541 cagagaccgg caatgacaac gagaaagaaa agcgcgggtt tccaccactg aatgggcgtt
     6601 gccgcctgag atgaagactg accagtcata gcattcccca ggaataagtt atgataattg
     6661 agcgcgtgaa tattacgctc actatcaatt cttgggaata attattcagc tcttttgcgt
     6721 aattctgaag agcttaaaat agtcacgcct tcatgctcag gccgcatcgc ctctgccagc
     6781 atgacccgcg ccacgtctct cgcatcaatg gatttccagt taccgggcag taaacgaaac
     6841 agcggcgcaa aaagcgtttc gttcatccgc tgtttgctac gatcgcccag caacatcgac
     6901 gggcgagcaa tggtcaattt cggccagttc tgggcaatta atgcttcttc catctcccct
     6961 ttgacgcggt tatagaaaaa cggcgagtgg gcattggcac ccatagcact gaccaccagc
     7021 atatgctgcg cgcccagtcg ccgcccggtt aatgcggtat ccactaccag cgtgtaatcg
     7081 gcatgaataa acgcctcttt gctccccgct tctcgccgcg tggtgccgag acaacaaaac
     7141 acaatgtcga tgggttcggt gacctgcgcc aacgcgtcac tcagttgcgg atcatgggga
     7201 ttaaacaccc cgggcatatc gcccaacgga cgtcgcgtcg gcgcggctat ggcgttaact
     7261 ttcggttcgt taatcaacat ccgcagcagg tgaccgccca ccagccccgt tgcgcctgta
     7321 atcagtacct gactcatctt cgctccttta cagaattgtc tgccttgcgc tccacggctc
     7381 atgcaccagg cttaatagac catgaggtaa ttatccccga ttgtgtgaaa ttcgcctcat
     7441 ccaatgcaac aacgcggagg aagcatgagt aagaaaattg ccgttttaat cagtgatgaa
     7501 tttgaggatt cagaatttac ttcacccgca gacgagttcc gtaaagccgg acacgaagtg
     7561 attaccattg aaaaacaagc gggtaaaacg gtgaaaggca aaaaaggaga agccagcgtg
     7621 accatcgata aatccatcga tgaagtgacg ccagcggagt ttgatgccct gctgctaccg
     7681 ggtggtcatt caccggatta tctgcgtggc gacaaccgtt ttgtcacctt tacccgtgat
     7741 tttgtgaata gcggcaaacc tgttttcgcc atctgtcacg gtccgcagct gctgatcagc
     7801 gccgatgtga ttcgggggcg caaactgacc gcagttaaac cgatcattat tgatgttaaa
     7861 aatgcgggcg cggaatttta cgatcaggaa gtcgtggttg ataaagatca gctggttacc
     7921 agccggacac cggacgatct gccagcgttt aaccgcgagg cgttacgcct gctcggtgcc
     7981 tgagtcgcgt agccaaatca tttttttacc aaagcccagc gtgttgtcgg tgaatttgat
     8041 ttcatcgagc cggatttccc acaccggggc cgacagcatt ctggcaaccg gaaagcgacg
     8101 attgtacgcc tggcgcgcga ggtcgctttc ctcaccttcc agcctgcgga tctcaccttt
     8161 aaactgcacg ccgcgaatta acgctaccgt tttcggctga ccgtttaccg ttccggcaac
     8221 cgcagcctgc ggcccactca tctgcgcgtg gcgcgttttt tcttccgtca aaatgtagaa
     8281 ggcgactttc tgcgcatcaa acagataaaa agcattagcg caccacagtt ccccttcctg
     8341 ctgcacacac caggtgacaa catgttgttt tgccaaccaa cggctgatgg tgatgagtgt
     8401 ttccattgct gctctctttt atactgtggg cctgacctta acatgcgacg cagacgatga
     8461 caccctggtt tctttacttg atccgtaccg ccgacaataa gctttatacc gggatcacca
     8521 cggatgtcga acgccgctat cagcagcacc aaagcggcaa aggggcgaaa gcactgcgcg
     8581 ggaaaggaga actgacgctg gcgttttctg cgccagtggg cgatcgttcg ctggcgttac
     8641 gggcggaata tcgcgttaag caactgacca aacggcaaaa agagcgtctg gtagcggaag
     8701 gcgcagggtt tgcagagctg ttaagcagtc tgcaaacccc ggagattaaa agcgattgaa
     8761 atgctcgtga tactcaacca gaccggttac gccattcagc gcgtcatccg ctaaacgatg
     8821 tacctggaag gcgctttcag tgcccggcca gcggcaacgc agatcgtgat gcgccgccag
     8881 ttcaaagccg aaacgactgt acagcgccgg atcgcccagc gtcaccactg cggcatagcc
     8941 gaactcatta agcgaatcga gtccttcata gaccagttgg cgtgccagcc cctgtccacg
     9001 gtatttttca tcaaccgcca acggtgccat gccgacccat tgcaggtctt cgccctgcac
     9061 atcaaccgga ctaaatgcca catagccaat gacctgacct tcgtcatctg tcgccaccag
     9121 ccccagcgtc agaaagccat cttcacgcag atcgtgaacc agcttcgctt ccgcatcact
     9181 ttcgaatgag cgacgcagca gggcatcaat acccggcgca tcaatgggaa tttctactcg
     9241 aattagcatg gttcacctac cgatgtctgt ttggtttcag gcgcggtttt cattcccgcc
     9301 tcaacaaaat ccgccagttg cagcagcatc atgcgtaagg ctttcggcat ttgctccagt
     9361 tcaatggcgt ccatcaggtt tttcacatac agccccagct ccgtatcgcc ttcaatcacc
     9421 agccgacgct ggaagaagag cgtatccgga tcttgtttac gcgccgcaat catcagcaga
     9481 tcgctggcgt cggcactaaa actcacatca gcttgcgcgt tctggctgac caccagtttg
     9541 ccattcacca ccgaggtaaa ccattgcagg tcaatatcac gcacatgaat acttaaccag
     9601 cggccttcaa gaaactccag ctcaccatca tccagcgcct gacggaattg ccagcttaag
     9661 acctgctcaa gaacctggcg ttttagcgca aatggcgtca gttttaccgg tacactcaac
     9721 agagatggcc ccaaatgcac aatacgggaa cgcagtttat ccaacacgag ttttactccc
     9781 tgtttcaaca atcatcctat tttgccatat cagaaaaata acatagcggt ataaatcaac
     9841 aattccatat gaaattgctg ctaccaccaa tacaacttta actgccttaa atcaaaaatt
     9901 gtcgcagcaa ggttaactaa aatcccagtt cgttaacatt tttgcgtttt gaaagcgcaa
     9961 ccttcaggaa aaattatgga gctgctctgc cctgccggaa atctcccggc gcttaaggcg
    10021 gccatcgaaa acggcgcaga tgctgtttat atcgggctaa aagatgatac taatgcccgt
    10081 cacttcgccg gccttaactt taccgagaaa aaattgcagg aagcggtgag ttttgtccat
    10141 caacatcgcc gcaaacttca catcgcgatt aacacttttg cgcatccgga cggttacgcc
    10201 cgttggcagc gcgccgtgga tatggcggcg cagctgggtg ccgacgcgct gatcctcgcc
    10261 gacctcgcca tgctggagta cgccgccgag cgttacccgc atattgagcg tcatgtgtca
    10321 gtgcaggctt cggcgaccaa tgaagaggcg attaactttt atcatcgcca ttttgatgtt
    10381 gcccgcgtgg tgctgccgcg cgtgttgtcg attcatcagg tgaaacagct ggcacgggtc
    10441 acacctgtcc cgctggaagt ctttgctttt ggcagcctgt gcattatgtc ggaaggtcgt
    10501 tgctatctgt cgtcgtatct gacgggtgag tcgcccaaca ccgtgggcgc gtgttctccg
    10561 gcccgtttcg tgcgctggca acaaacgccg caggggctgg aatcccgcct gaacgaagtg
    10621 ctgatcgacc gttatcagga tggcgaaaac gcaggttatc cgacgctgtg taaagggcgt
    10681 tatctggtgg acggcgagcg ctatcacgcg ctggaagaac caaccagtct caataccctg
    10741 gaactgctgc cggagttaat ggcggcgaat attgcttcgg tgaaaattga aggccgccag
    10801 cgtagcccgg cgtatgtcag ccaggtggcg aaagtctggc gtcaggctat cgaccgttgt
    10861 aaggccgatc cgcaaaactt cgtaccgcaa agcgcgtgga tggagacgct cgggtcgatg
    10921 tccgaaggca cgcagaccac ccttggcgcg tatcaccgta aatggcagtg agaagagcaa
    10981 tgaaatattc cttagggcca gtgctgtggt actggccaaa agaggcgctg gaagaatttt
    11041 atcagcaggc cgccaccagc agcgccgacg tgatttatct tggtgaagcg gtatgcagca
    11101 agcgccgggc aaccaaagtt ggcgactggc tggagatggc aaaatcgctc gccggaagcg
    11161 ggaagcagat agtgctctcc acgctggcgc tggtgcaggc atcctctgaa ctgggcgaac
    11221 tgaaacgcta tgttgagaac ggtgagtttc tgattgaagc cagcgatctc ggcgtggtga
    11281 atatgtgcgc tgaacgcaaa ctgccgttcg tcgccgggca cgcgctgaac tgctacaacg
    11341 cggtgacgct gaaaatattg ctcaaacagg gcatgatgcg ctggtgtatg ccggtggagc
    11401 tttcccgcga ctggctggtg aatctgctta atcagtgcga tgagttgggg attcgcaacc
    11461 agtttgaagt ggaagtcctg agctacggtc atctaccgct ggcctactcc gcccgctgct
    11521 ttaccgcgcg ttcggaagac cgcccgaaag atgagtgtga aacctgctgc attaagtatc
    11581 cgaacgggcg caacgtgctg tcgcaggaaa accaacaagt gtttgtactc aatggcattc
    11641 agaccatgag cggctacgtt tacaacctcg gtaacgagct gacatccatg cagggattgg
    11701 ttgatgtggt ccgcctgtca ccgcagggta ctgacacttt cgcgatgctc gacgccttcc
    11761 gcgctaatga aaatggcgct gcgccactgc cgttgacggc aaacagcgac tgtaacggct
    11821 actggcggcg gctggctggg ctggagctgc aagcctaagt aaatatgctc gtcatacttc
    11881 aagttgcatg tgctgcatct gcattcgttc accccagtca cttacttatg taagctcctg
    11941 gggattcact ctcttgtcgc cttcctgcaa ctcgaattat ttagagcaca gctcactttg
    12001 ttaacaactt taactactct ttaatgcagt attaaagatt aatcggtaac aaagtgagct
    12061 gttatgactg ataaaaccat tgcgttttcg ctactcgatc tggcccccat tcccgaaggt
    12121 tcttcagcgc gagaagcatt ctcccactct ctcgatctcg cccgtctggc tgaaaagcgc
    12181 ggctatcatc gctactggct ggcagaacac cacaatatga ctggcattgc cagtgctgcc
    12241 acgtcggtat tgattggcta tctggcggcg aataccacca cgctgcatct ggggtctggc
    12301 ggcgtgatgt tgcctaacca ctcaccgttg gtcattgccg aacagttcgg cacgctcaat
    12361 acactctatc cggggcgaat cgatttgggg ctgggtcgtg cgccaggtag tgaccaacgg
    12421 acaatgatgg cgctgcgtcg ccatatgagc ggcgatattg ataatttccc ccgcgatgtc
    12481 gcggagctgg tggactggtt tgacgcccgc gatcccaatc cgcatgtgcg cccggtacca
    12541 ggctatggcg agaaaatccc cgtgtggttg ttaggctcca gcctttacag cgcgcaactg
    12601 gcggcgcagc ttggtctgcc gttcgcgttt gcctcacact tcgcgccgga tatgttgttc
    12661 caggcgctgc atctttatcg cagcaacttc aaaccgtcgg cacgactgga aaaaccatac
    12721 gcgatggtgt gcatcaatat tatcgccgcc gacagcaacc gcgacgctga atttctattt
    12781 acctcaatgc agcaagcctt tgtgaagctg cgccgtggcg aaaccggaca actgccgccg
    12841 ccgattcaaa atatggatca gttctggtca ccgtctgagc agtatggcgt gcagcaggcg
    12901 ctgagtatgt cgttggtggg tgataaagcg aaagtgcgtc acggcttgca gtcgatcctg
    12961 cgcgaaaccg acgccgatga aattatggtt aacgggcaga ttttcgacca ccaggcgcgc
    13021 ctgcattcgt ttgaactggc gatggatgtg aaggaagagt tgttgggata gtgtgtctta
    13081 acgcgggaag ccttatccga gctggcaacg ctgtcctacg tagacctgat aagcgaagcg
    13141 catcaggcat tgtgtaggca gcagaaatgt cggataaggc accgctgatt actgatacac
    13201 cggcagcaaa ttaaagctcg ataagatatg gaccagtgcg ttgccaacgc caaacaccag
    13261 aatcagcgta atcatcggct tgccgcccca gacacggaat ttcgggctgc caaagcgttt
    13321 acgcgatgcg cgggctaaca gcgccggaac aattgccgcc cagatggtag ctgctaaacc
    13381 agcataacca atggcgtaca ggaatccgtt cgggaacagt agaccaccca caacaggcgg
    13441 ggcaaaggtc agcaatgccg ttttcaagcg ccccagggct gagtcatcga aaccaaacag
    13501 atctgccaga tagtcaaaca aacccagcgt tacgccaagg aacgaactcg ctaccgcaaa
    13561 gtttgagaac acgaccagca gaaggtccag gctacggctg ttcagcacgc cgcttaatgc
    13621 ctgtaccagc acatcaatat taccgccctt ctctgcaata ccgataaact ccggacgcgg
    13681 gatgttaccc atcgtcgcca gcaaccagat ggtatacagc gccagtgcca tcagcgtacc
    13741 gtacaccagg catttcacga tggttttcgg atctttgccg taatacttca tcaggctcgg
    13801 cacgttaccg tgataaccaa acgatgccag acagaacggc agggtcatca gcaaatacgg
    13861 cgcataagat gcattgcttt cggcgacgtt gaacaatgtc gcaggctgca catgccccag
    13921 taggctaccg aaggtgagga agaaggtaat gactttcgcc cccagtacaa tcgccgtcat
    13981 gcggctgacg gccttagtac tcaaccacac cacaaacgct accagcagtg caaaaccaaa
    14041 acccgccgcc cgtgccggga cgtttagcga catctctgcg aaggtgtgat gcagaattga
    14101 accactggca gaaatatagg cataggtcag gatatagagc acaaaggcaa tggaaatacc
    14161 gttgaccacg ttccagcctt tgcccagcaa atctttggtg atggtgtcaa aactcgaacc
    14221 gattcggtaa ttcaggttag cttccagaat catcaagccg gaatgcagca tacagaacca
    14281 ggtaaagatc agcgccgcca ttgaccagaa aaaccacgcc ccggacatga ccactggcag
    14341 agaaaacatc cctgcgccaa taatggtgcc gccgataatc accacgccgc caagcagcga
    14401 cggtgacgtt tgggtggtgg ttagtgttgc catgagagct tctctccagt gaaaaatagt
    14461 gcgactgcgt tgttatgcat tgcactgtac cagtacacga gtacaaaaga cagaaaaaaa
    14521 gccccgatgg taaaaatcgg ggctgtatat attattttac agattgtgtt cgctgttcag
    14581 cgatgattac gcatcaccac cgaaacgacg acgaccggta gagtcatcac gacgcggagc
    14641 gcggccttca cgacgttcgc cgctaaaacg acgaccatca ccacggccac cttcacggcg
    14701 ttcaccgctg aagttacgac cgccttcacg acgttcgcca ccgaaaccac gaccaccgcc
    14761 acgacgctca ccgccagtat gcggctgtgc atcgcccagt aactgcatgt tcatcggctt
    14821 gttgagaatg cgagtgcgcg taaagtgttg cagcacttca cccggcatac ctttcggcag
    14881 ttcgatggtg gagtgagaag caaacagctt gatgttacca atgtaacggc tactgatgtc
    14941 gccttcgtta gcaatcgcac caacgatatg acgaacttca acaccatcat cgcggcccac
    15001 ttcaatgcgg tacagctgca tatcgccaac atcacgacgt tcacgacgcg gacgatcttc
    15061 acggtcacca cgcgggccac ggtcgttacg atcgcgcgga ccacggtcat cacggtcacg
    15121 gaattcacgt ttcggacgca tcggcgcatc tggcggtacg atcagagtac gttcaccctg
    15181 tgccattttc agcagtgccg cagccagagt ttcgagatcc agctcttcac cttctgcagt
    15241 cggctgaatt ttgctcagca gtgcgcggta ttgatccaga tcgctgcttt ccagctgctg
    15301 ctgtacttta gcggcgaatt tttccagacg gcgtttgccc agcagttctg cgttcggcag
    15361 ttctacttcc ggaatagtca gcttcatagt acgttcaatg ttgcgcagca gacgacgctc
    15421 gcggttctca acgaacagca gcgcgcggcc agcacgaccc gcacgaccgg tacgaccgat
    15481 acggtgaacg taagactcag aatccatcgg gatatcgtag ttaactacca ggctgatacg
    15541 ctcaacgtcc aggccacggg ctgcaacgtc ggtcgcaatc aggatgtcca gacgaccatc
    15601 tttcaggcgt tccagtgtct gttcacgcag cgcctggttc atgtcaccgt tcagcgcggc
    15661 gctgttgtag ccgttacgct caagagcttc agccacttcc agagtcgcgt ttttggtacg
    15721 aacgaagata atcgccgcat caaaatcttc cgcttccagg aaacgtacca gtgcttcgtt
    15781 tttgcgcata ccccagacag tccagtagct ctggctgatg tcaggacggg tagtcacgct
    15841 ggactgaatg cgcacttcct gcggctcttt cataaagcgg cgggtaatgc gacgaatcgc
    15901 ttccggcatg gttgcagaga acagagcggt ctgatgacct tccgggatct gcgccataat
    15961 ggtttcaacg tcttcgatga agcccatgcg cagcatttcg tcagcttcat ccagaaccag
    16021 accgctcagt ttagagaggt ccagagtgcc acgtttcagg tggtccagca gacggcccgg
    16081 agtaccgaca acgatctgcg gcccctgacg cagggcgcgt aattgcacgt cataacgctg
    16141 gccgccgtac agagcaacca catttacgcc gcgcatgtgt ttagagaaat ccgtcattgc
    16201 ttcagcaacc tgtaccgcca gttcgcgggt cggtgccagc accagaatct gtggtgcttt
    16261 cagctcagga tcaagattct gcaacagagg taaagagaat gctgcagttt ttccgctccc
    16321 cgtctgggcc atacccagaa cgtcgcggcc attcagcaga tgtggaatac actctgcctg
    16381 aattggagat ggtttttcgt aacccagatc gttaagggct tcaaggatag gagccttcag
    16441 gcccagatct gcaaaagtgg tttcgaattc agccatgtag tacgtgtgcc tcaaaattaa
    16501 tggcggccag tctacataac tcatcatgaa attgatcagc aattttcatt gaaaagtgtg
    16561 aaccggctca aagtaggtgt attaacgaac aacaacgccc tcacccgtta aggtgatggc
    16621 aatcaaaaaa gattacgggc tgatgtgtac gtcagctatt gctggtccga ttctgccagg
    16681 tcatcttggt cctggcccag gagcgataat tccaacaatg cgtatcggtg ctcaacaaag
    16741 ttatgaacgt tgttggcaac cgccagtttg aacagtgccg tggcgctgtc caaatccccc
    16801 agacttaggt agtacttacc taaatagaag ttggtttcac tgagatgctc agcgagcgag
    16861 gtgttatccg ttgcgtccgc cttgagcctt tccattaacg tttgttcgct aatgttgccc
    16921 aagtagaact cgacaatgtt ccatccccac tgttccttat ccgatttttc gaagtgctgt
    16981 ttcaacactt ctttagcctg cttctcatcg agcttctgct cggcgagata aagccacaga
    17041 ctacggaaag gatcattggg atcgtcttga taaaacgcca gcagatcatc ttgcgctaac
    17101 ttgtcacgac cgccgtaata taatgcgatc ccgcgattca agtgcgcgta gttgtaagtt
    17161 ggatcaagct caagtacaga atcaaacgct tcataggcag catcaaaatt gcctgcctgc
    17221 gttaaatata tgcctaagta attgaatact tcaggcatat ccggtcggat tgccagcgct
    17281 tgcgaaaaat cgttacgcgc taatgccctc agaccgagac tatcatacaa cactccgcgc
    17341 tcatataaaa gctgtgcgcg ttcgtcatcg gttaaagccc gactggcaag gatttgttcc
    17401 atacgtgcca gaatcacttc ctgctgtaaa gtcggttgca atggtaccgc gaggacttca
    17461 cttttacgcc aggaagtatt actgcatcct gcaagcgtaa gtgctgtcgc aacgaaacac
    17521 cagcgcaaaa aaggcttcat ttcccactcc cgaagaccac ggttgaatga acgtcctgtt
    17581 cccggttgct aacaaggcgt cctgcccggt taaaagcccc ccgccgcagc ggagggcaaa
    17641 tggcaacctt actcgccctg ttcagcagcc ggagcttccg gtgctgcagc aggttgagac
    17701 tgctcagtcg cttctttaat gctcagacgg atacggccct ggcgatcaac ttctagaact
    17761 ttcaccggta cttcctgacc catctgcagg taatcggtca ctttctcaac gcgtttgtca
    17821 gcgatttgag agatgtggac cagaccttct ttaccgccgc cgatggcaac aaatgcgcca
    17881 aagtcaacga tacgggtcac tttaccagtg tagacgcggc ccacttcgat ttctgcagtg
    17941 atctcttcga tacgacgaat agcatgtttc gctttctcgc cgtcggtcgc tgcgatcttc
    18001 acagtaccgt catcttcgat ttcgatggta gtgccagttt cttcggtcag ggcacggata
    18061 acagagccgc ctttaccgat aacgtctttg atcttatccg ggttgatctt gatggtatgg
    18121 atacgcggtg cgaactcaga gatatcgcca cgcggcgcgt tgatcgcctg ttccattacg
    18181 cccaggatat gcagacgcgc acctttagcc tggttcagcg caacctgcat gatctctttg
    18241 gtgatacctt caattttgat atccatctgc agtgcagaga taccgtcgcg ggaacctgca
    18301 actttgaagt ccatatcgcc caggtgatct tcgtcgccca aaatgtcaga cagtacaacg
    18361 tagttgtcgc cttctttcac cagacccatt gcgatacccg caacggcagc tttgattggc
    18421 acacctgcgt ccatcagcgc cagagacgcg ccgcacacgg aagccataga agaagaaccg
    18481 ttggattcgg tgatttcaga cactacacgt acggtgtacg ggaatttgtc catatccggc
    18541 atgactgcca gcacgccgcg cttcgccaga cgaccgtgac caatttcacg acgcttcgga
    18601 gaaccgacca tgccggtttc gcctacggag tacggaggga agttgtagtg gaacaggaag
    18661 gtatcggtac gttcgcccat cagttcatca agaacctgcg cgtcacgagc ggtacccagc
    18721 gttgcagtaa ccagtgcctg cgtttcaccg cgggtgaaca gcgcagaacc gtgagtacgc
    18781 ggcagcacgc cagtacgcac atccagacca cggatcatat ctttttcacg accgtcgata
    18841 cgcggttcgc ctgccagtac gcggctacga acaacgtttt tctcgatagc gtgcagaatt
    18901 tcacccagtt cgttttcgtc cagggtttcg tcttcagcaa gcagcgtcgc gatggtttca
    18961 gatttgatga catcaacctg cgcataacgc tcttgtttgt cggtgatgcg gtaagcatca
    19021 ctcagacgag cttcagccag tgcagcaacg cgcgcgttca gcgcttcgtt taccggctcc
    19081 ggctgccagt cccaacgcgg tttaccggct tctttcacta gttcattgat gttctgaata
    19141 acaacctgct gttgttcatg accgaacact actgcgccca gcatctgatc ttcgctcagc
    19201 agttcagctt cagattcaac catcagtaca gcggcttcag taccggcaac aaccagatcc
    19261 agtttgctct ctttcagctc gtcctgagtc gggttcagta cgtactggtc attgatgtaa
    19321 cctacgcggg cagcaccaat cgggccattg aacggaatac cagacagaga cagtgctgcg
    19381 gaagcaccaa tcatcgcgac gatatccggg ttaacctgcg ggttaacaga aaccacggtg
    19441 gcgataacct gaacttcgtt gacgaagcct tccgggaaca gcgggcgaat cgggcggtca
    19501 atcagacgcg cgatcagggt ttcgccttcg cttggacgac cttcacgacg gaagaagcta
    19561 cccgggatac gaccagcagc gtaggtacgc tcctgatagt taacggtcag tgggaagaag
    19621 tcctgacctg gtttggcttt tttctgtcca acaacggtaa cgaataccgc ggtgtcatcc
    19681 atgctaacca taacagcggc agtagcctga cgagccatca tgccggtttc cagagtcacg
    19741 gtgtgttggc cgtactggaa tttacgaacg atcggattaa gcaatgtaat atcctttctc
    19801 tttcttagac agtaccttac ggcactggtg ttaatacccg atcttctgcg catcctcgcg
    19861 actaatgaca accctaaccc agctctatgt gggtaaagcc tctcattagc cgcgcgaacc
    19921 tctgcaacgg aagatcattc atagcaacaa tacattagtt tccagtgaat tgctgccgtc
    19981 agcttgaaaa aaggggccac tcaggccccc ttttctgaaa ctcgcaagaa ttagcgacgc
    20041 agacccaggc gctcgatgag ctgggtgtaa cgtgctacgt ctttacgttt caggtagtcg
    20101 agcagtttac gacgctgaga aaccatgcgc agcagaccac gacggctgtg gtgatctttt
    20161 ttgtgctctg caaagtggcc ctgcaggtgg ttgatctgtg cagtcagcag tgctacctga
    20221 acttcggtag aaccggtgtc gtttgcgtca cgaccaaact cagaaacgat tttagctgtt
    20281 gcttcagtac ttagagacat tttaaaactc caaagtatat agaatgaaag gacgccgatc
    20341 tctaattcag cgatcccagt gtacgttacg caaagtgtta aacaatttac gcgacgttaa
    20401 gcggcagtat tctactcgta gcgacctgtt atcgcaagac ggttaacatt acgccgggta
    20461 ttcaaccacc aggcgacgag gcgcgacgcg gccttcatcg tcaatttcgc ccataccgat
    20521 aaacttgccg ttctcacctt ctgtaacgcg aaccagtcct tccagcggcg caccggatgt
    20581 acgaactggg ttaccatttt tgaagtaaac agaagacgtt aacggaagat tcaccaccgg
    20641 gtagtccgaa gctggactgt ccattggcat cagtaatgga tcaagtaact ccgcagccgg
    20701 aatatcctgc tgttcggctt gttcgaccag ttcacgcaga tgttccaggg tcaccatccg
    20761 ttcaaccgga tatttactta ccgccagacg acgcaggtaa ataacatgcg cgccacaacc
    20821 gagtttttca cccaggtcat caatgatggt gcggatataa gtgccttttg agcagtgaat
    20881 ttccagctcc agctcattgc cttcgtggcg aataaacagc aattcataaa cggtaatcgg
    20941 gcgcgcttca cgcggtactt caatgccctg acgcgcatat tcgtacagtt ttttaccctg
    21001 atatttcagt gctgaataca tcgaagggat ctgttcgata tcgccacgga aagtatccag
    21061 cgccgccgcc agttgctctg cgctaaaggt taccggacgt tcttcaacga tttgtccgtc
    21121 ggcatcagaa gtatcggtac gctgtccaag acgcgcaatg acccgatagc gtttgtcgga
    21181 gtccagcaga tactgggaaa acttcgtcgc ttccccgagg caaattggca acatgcctgt
    21241 tgccagcggg tccagcgcac cggtatgccc ggcacggttg gcgttataaa tccgtttcac
    21301 tttttgcagc gcatcattgc tggacatacc ctgcggttta tccagcaaca aaacgccgtt
    21361 aatgtcgcga ccgcgacgac gaggacgact cattagtcct ccttgctgtc gtccgggtta
    21421 acacgacgtt cttcgtcatg tttgaccacg ctggtcacca ggtttgacat gcgcatccct
    21481 tcaaccagag agttgtcgta gaagaaggtc agttccggca cgatacgcag gcgcatcgct
    21541 ttccccagca ggctgcggat gaaaccagaa gcttcttgca acgctttgat gcccgcttta
    21601 accgcgtctt catctttgtc gttgaggaac gtcacatata ctttggcata cgccaggtcg
    21661 cgagacattt cgacaccgga aacggtggtc atcatgccca ggcgaggatc tttaatttca
    21721 cgctgcagga tgagagcgat ctctttttgc atttcctgcg ctacgcgctg cgggcgacca
    21781 aattctttcg ccataataaa ttctcctgac aaaaaagggg ctgttagccc ctttttaaaa
    21841 ttaatttcag gtggaagggc tgttcacgtt ggcctgataa gacgcgccag cgtcacatca
    21901 ggcaatccat gccggatgcg gcgtaaacgc cttatcccgc atggaaccct aaaaacctta
    21961 agcgatggta cgttggatct cgatgatttc gaatacttcg atcacatcgc cagtgcggac
    22021 gtcgttgtag ttcttaacgc cgataccaca ttccataccg ttacggactt cgttaacgtc
    22081 atctttgaag cggcgcaggg actccagctc gccttcgtag ataaccacgt tgtcacgcag
    22141 aacgcggatc gggttgtgac gtttaaccac accttcggta accatacagc ctgcgatagc
    22201 accaaatttc ggcgatttga acacgtcacg aacttccgcc agaccgataa tctgctgttt
    22261 cagttccgga gacagcatac cgctcatcgc cgctttcact tcgtcaatca ggttatagat
    22321 gacggagtag taacgcagat ccaggctttc cgcttcaatc actttacgtg cagaggcatc
    22381 agcacgtacg ttgaagccaa ccaggatggc gttggacgcc gcagccaggg tggcgtcggt
    22441 ttcggtgata ccacctacgc cagaaccgat gatcttcact ttaacttcgt cagtagacag
    22501 tttcagcaag gagtcggaga tcgcttccac agaaccctgt acgtctgcct tcaggacgat
    22561 gttcacttcg tgaacttcgc cttcggtcat gttggcgaac atgttctcga gtttagattt
    22621 ctgctgacgc gccagtttaa cttcgcggaa tttaccctga cgatacagtg caacttcacg
    22681 cgctttcttc tcgtcacgta caacggtaac ttcatcaccc gcagccggta cgccggacag
    22741 gccgaggatt tccaccggaa tggacggacc cgcttccagc acttcctgac ccagttcgtt
    22801 acgcatcgca cgaacacgac cgtattcgaa gccacacaga acgatatcgc ccttgtgcag
    22861 agtaccttca cgtaccagaa cggtagcaac cggaccacga cctttatcga ggaaggattc
    22921 gataaccgca ccgctcgcca tacctttacg taccgctttc agctccagaa cttccgcctg
    22981 cagcaggata gcgtccagca gttcatcgat gccggtaccc gctttcgcag atacgtgtac
    23041 gaactggctt tcaccgcccc actcttccgg caggatgcca tactgggaga gttcgttctt
    23101 aacacgatcc ggatcggctt ctggtttatc aatcttgttc actgcaacca ccaccggtac
    23161 ctgcgccgct ttcgcgtgct ggattgcttc gatggtctgc ggcatcacgc cgtcgtcggc
    23221 agcaacaacc aggactacga tgtccgttgc ctgcgcacca cgtgcacgca ttgaagtaaa
    23281 cgcggcgtgc cccggggtgt ccaggaaggt gatcatgccg ttttcagttt caacgtggta
    23341 tgcaccaatg tgctgggtaa tgccgcccgc ttcgccagag gcaactttcg ttgaacgaat
    23401 gtagtccagc agagaggttt taccgtggtc aacgtgaccc atgatggtca caaccggcgc
    23461 gcgcggttca gccgcagcac ccgtgtcacg gtcgctcatt accgcctctt ccagctcgtt
    23521 ttcacgacgc aggataactt tgtggcccat ctcttcagca accagctgtg cggtttcctg
    23581 atcgataacc tggttgatgg ttgccattgc gcccagtttc atcatcgctt tgatgacctg
    23641 agagccttta accgccatct tgttcgccag ttcgccaacg gtgatggttt cgccgatcac
    23701 aacgtcacgg ttaacggcct gagctggctt ctggaagcct tgctgcaggg aagaaccttt
    23761 acgttttccg cctttaccgc cacgtactgc tgcgcgtgct tcttcacgat cagcttttga
    23821 ttcagcgtgt ttgttgcctt tcttcggacg cgctgctttc gcgttacgac cacggccacg
    23881 accgccttcg acttcacgat cgctttcgtc ttctgcctgg cgagcatgtt gagaagtagt
    23941 gacgtgataa tcgctggaat cttcagtcgg ttccgcgtta tcagtccatt tgttttcttc
    24001 cgccatacga cgtgcttctt cagcaacgcg acgtgcttct tcttcgagtt tacgatgcgc
    24061 ttcttcttcg gctttacgct tgagctctgc agcttcctgc tcacggcggg ctttttcagc
    24121 ctgggcgttt ttagtcatat cgtcttgttg attgctcact ttgtcttttt ccgcagcttc
    24181 acgtttcgct tgttcagcag cttcacgctt agcttgttct gcagcctcac gttcagcttt
    24241 ttgttgcgcc tcgcgtttag ccgattcttc tgcctcacga cgggcttgct cttccgcttc
    24301 acgctgcgct tgctcttccg ctgcaaggcg ttcagcctct tgcggatcgc gtttcacaaa
    24361 ggtgcgtttc ttgcggactt cgatttgtac cgatttgctt tttccaccgg taccaggaat
    24421 gttaagggtg ctgcgtgttt tacgttgcag cgtcaatttg tccgggcctg aatttttctg
    24481 attcaggtgg tcaatcaaag tctgtttctc ttgtgcagac acagagtcgt cagcagactt
    24541 ccggatacct gcatcagcaa attgctgtac caggcgttcc acggaggtct gtcgctctgc
    24601 ggccagcgtt ttaatcgtta catctgtcat gctgttcctt cctgctacag tttattacgc
    24661 ttcgtcaccg aaccagcaaa tattacgggc agccataatc agtgctccgg ccttttcgtc
    24721 ggtcaaccct tcgatatcag ccagatcatc aatgccctgt tcggcgagat cttccagcgt
    24781 acaaacgcca cgggcggcca gtttgaatgc caaatcacga tctacccctt caaggttcag
    24841 cagatcgtca gccggtttgt tatcaccgag gctttcttcc tgggcctgtg caatggtggc
    24901 cagtgcattt ttagcacgct cgcgcagtgc ttcaacggtc ggctcatcaa ggccttcgat
    24961 ttccaacagc tctttcatcg gcacataggc cagttcttcc agcgtcgaga agccttcttc
    25021 taccagaaca gtcgcgaagt cttcgtcgat gtcgagatat ttggtgaagg tgtcgatcgc
    25081 tgcgtgcgct tccgcctgat gcttagcctg caggtcgtca acggtcatca cgttgagttc
    25141 ccaaccgctc agctgcgaag ccagacgcac gttctgaccg ttacggccaa tcgcctgcgc
    25201 caggttaccg gcttcaacgg cgatatccat ggtgtgttta tcttcatcca ccacgataga
    25261 agcaacgtct gccggtgcca ttgcgttaat cacgaactgc gccgggttat catcccacag
    25321 gacgatatcg atacgctcgc cacccagttc agtagacacc gcctgaacac gcgcgccacg
    25381 catacctacg caagcaccta ccggatcgat acgtttatcg ttggttttca ccgcgatttt
    25441 cgcacgagaa cccggatcgc gagccgctgc tttaatttca atcacttctt cgccgatttc
    25501 tggcacttca atacggaaca gttcgatcag catttccggc ttggaacgag tgacgaacag
    25561 ttgcgcgcca cgcgcttccg ggcgaacgga ataaagcacg ccacgaacgc ggtcgccagg
    25621 gcggaagttt tcacgcggca gcatatcttc acgcaggatc acggcttcag cgttgttgcc
    25681 cagatccaga gagatgttgt cgcggtttac ttttttcacc acgccggtga tgatttcacc
    25741 ttcgtgttca cggaactgat caaccaccat cgcacgttcg gcttcacgca ctttctgcac
    25801 gataacctgt tttgccgtct gggtagtgat acggtcaaag gtaacagact caatctgatc
    25861 ttcaacgtaa tcgcccaggt tcaggctttc atcttcataa cgtgcggctt caagggtgat
    25921 ttccttggtc ggctgggtga cttcatcaac aactaaccag cgacggaagg tgtcaaaatc
    25981 accgcttttg cgatcgatct gtacgcggac gtcgatctct tgttcatatt ttttctttgt
    26041 tgctgtcgcc agcgcgcttt ccaatgcttc gaaaatcttc tcgcgaggta gcgccttttc
    26101 attggatacg gcttcaacta cagccaaaat ttctttgttc atcgcgggct tttcacctca
    26161 tccagactat taaaagtggg gaaccaggtt cgccttctgg atattactca gcgcgaacac
    26221 ttcatcttta ccttcgacgg taactgtgat catttcaccg tctaccgctt tgataacgcc
    26281 ctgccattta cgacggtttt gtaccgccat acggagaacc agagtcacct cttctccgac
    26341 aaaacgggcg tagtgttcag ccgtgaacag tgggcgatcg agacccggtg aggagacttc
    26401 caggttataa gcaacggtga tgggatcttc aacatccagc acagcactta cctggtggct
    26461 cacatcagca caatcatcaa cattgatgcc atcttcacta tcaatataga tgcgcagtgt
    26521 ggatgtgcga ccgcgaataa attcgatgcc aaccagttca aaacccaggg cctcaactgg
    26581 cgcagtaatc atctctgtta atttttgctc taatgtggac aagcccaccc ccaagacata
    26641 aaaaaagggc ctaaagccca gttattctgt agtcagataa caaaaaaccc cgataaatcg
    26701 gggctttata taactgaacc ctataaccgc aactgcggtc tggagcactt tccagaagga
    26761 ttttttcaaa tcccactacg aaggccgaag tcttcacagt atatttgaaa aaggactcta
    26821 agggaaa
//
LOCUS       DAJVQG010000043        26142 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJVQG010000043 DAJVQG010000000
VERSION     DAJVQG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26142
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.043"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            363..1262
                     /gene="lysO"
                     /locus_tag="OQM83_001632"
     CDS             363..1262
                     /gene="lysO"
                     /locus_tag="OQM83_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCS1519918.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1406..3058
                     /gene="hcp"
                     /locus_tag="OQM83_001633"
                     /gene_synonym="priS"
     CDS             1406..3058
                     /gene="hcp"
                     /locus_tag="OQM83_001633"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCS1519919.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            3070..4038
                     /gene="hcr"
                     /locus_tag="OQM83_001634"
     CDS             3070..4038
                     /gene="hcr"
                     /locus_tag="OQM83_001634"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCS1519920.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            4171..5889
                     /gene="poxB"
                     /locus_tag="OQM83_001635"
     CDS             4171..5889
                     /gene="poxB"
                     /locus_tag="OQM83_001635"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCS1519921.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            5926..6927
                     /gene="ltaE"
                     /locus_tag="OQM83_001636"
     CDS             5926..6927
                     /gene="ltaE"
                     /locus_tag="OQM83_001636"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCS1519922.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            6938..8368
                     /locus_tag="OQM83_001637"
     CDS             6938..8368
                     /locus_tag="OQM83_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCS1519923.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8467..9480
                     /locus_tag="OQM83_001638"
     CDS             8467..9480
                     /locus_tag="OQM83_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS1519924.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9477..10307)
                     /gene="amiD"
                     /locus_tag="OQM83_001639"
     CDS             complement(9477..10307)
                     /gene="amiD"
                     /locus_tag="OQM83_001639"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCS1519925.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10304..10627)
                     /locus_tag="OQM83_001640"
     CDS             complement(10304..10627)
                     /locus_tag="OQM83_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCS1519926.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(10838..12607)
                     /locus_tag="OQM83_001641"
     CDS             complement(10838..12607)
                     /locus_tag="OQM83_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCS1519927.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIA
                     LMNTFYDSDNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGFFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNIINNVFKNVSFRSVKINNVNTHSLKFINCHFFNVDMIRVNFSKCLFH
                     DCSMHGVKIKPWLPVKWTKELINDYLYGCLLSLYSICARDIYNMSAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQERAFIYEFARMIFAYINDKSIEILLLSCFAASDQKAIQRY
                     IPQSQDGEDFRSHLQYKLPLSAH"
     gene            13256..13771
                     /locus_tag="OQM83_001642"
     CDS             13256..13771
                     /locus_tag="OQM83_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1519928.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            13989..14717
                     /gene="artP"
                     /locus_tag="OQM83_001643"
     CDS             13989..14717
                     /gene="artP"
                     /locus_tag="OQM83_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCS1519929.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            14735..15466
                     /gene="artJ"
                     /locus_tag="OQM83_001644"
     CDS             14735..15466
                     /gene="artJ"
                     /locus_tag="OQM83_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1519930.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            15473..16189
                     /gene="artQ"
                     /locus_tag="OQM83_001645"
     CDS             15473..16189
                     /gene="artQ"
                     /locus_tag="OQM83_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCS1519931.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            16189..16857
                     /gene="artM"
                     /locus_tag="OQM83_001646"
     CDS             16189..16857
                     /gene="artM"
                     /locus_tag="OQM83_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCS1519932.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            17149..17880
                     /gene="artJ"
                     /locus_tag="OQM83_001647"
     CDS             17149..17880
                     /gene="artJ"
                     /locus_tag="OQM83_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCS1519933.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(18055..19182)
                     /gene="rlmC"
                     /locus_tag="OQM83_001648"
     CDS             complement(18055..19182)
                     /gene="rlmC"
                     /locus_tag="OQM83_001648"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCS1519934.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(19223..19711)
                     /locus_tag="OQM83_001649"
     CDS             complement(19223..19711)
                     /locus_tag="OQM83_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCS1519935.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(19771..20616)
                     /gene="potI"
                     /locus_tag="OQM83_001650"
     CDS             complement(19771..20616)
                     /gene="potI"
                     /locus_tag="OQM83_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCS1519936.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDGITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(20613..21566)
                     /gene="potH"
                     /locus_tag="OQM83_001651"
     CDS             complement(20613..21566)
                     /gene="potH"
                     /locus_tag="OQM83_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCS1519937.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(21576..22709)
                     /gene="potG"
                     /locus_tag="OQM83_001652"
     CDS             complement(21576..22709)
                     /gene="potG"
                     /locus_tag="OQM83_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCS1519938.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(22804..23916)
                     /gene="potF"
                     /locus_tag="OQM83_001653"
     CDS             complement(22804..23916)
                     /gene="potF"
                     /locus_tag="OQM83_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCS1519939.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(24267..24743)
                     /locus_tag="OQM83_001654"
     CDS             complement(24267..24743)
                     /locus_tag="OQM83_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCS1519940.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(24831..25733)
                     /gene="rimK"
                     /locus_tag="OQM83_001655"
     CDS             complement(24831..25733)
                     /gene="rimK"
                     /locus_tag="OQM83_001655"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCS1519941.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(25794..>26142)
                     /gene="nfsA"
                     /locus_tag="OQM83_001656"
     CDS             complement(25794..>26142)
                     /gene="nfsA"
                     /locus_tag="OQM83_001656"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCS1519942.1"
                     /translation="GVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWPADNPDLKPR
                     LPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRRTIIKESRPF
                     ILDYLHKQGWATR"
BASE COUNT         6473 a         6820 c         6449 g         6400 t
ORIGIN      
        1 ggccaatcgc cgccgccccc tgcccccccg ggctctggcg aacaaaatcg ccgctgcgcg
       61 gtgccctcga cttatccctt acggctaccg ggtcgggcgc gaggtaacat ccctgtaaaa
      121 cgcgccctca gcccacatcc atgtgggctg ccccggcctt cagggaacgc ctcggcgatt
      181 ttgacgccac caacatcggt gcagtttgta attaaagcgc aagaatatct ttaaatgaaa
      241 acgaatactc tactgctcct tatgcataac atttcctttt aattgcaact ttactcgtcc
      301 ttccgctttc agtagtgtgt catcgcgcat ttcgacttca tctttgcaag gactgggttt
      361 tcatgttttc tgggctgtta atcattctgg ttcccctgat tgtaggttac ctcattccgc
      421 ttcgccaaca agctgcgtta aaagttatta atcagctatt aagctggatg gtttacctta
      481 ttctcttttt tatgggtatc agtctggcgt ttctcgataa cctcgccagt aacctgttgg
      541 cgattctgca ttattctgcc gtcagtatta ccgttatttt actgtgtaat attgccgccc
      601 tgatgtggct ggagcgaggc ctgccgtggc gcaaccacca tcagcaagaa aaactcccgt
      661 cgcgtattgc gatggcgctg gagtcgctaa aactgtgcgg cgtagtagtg attggttttg
      721 ccattggtct gagtggactg gctttcttac aacacgcgac cgaagccagt gaatacacgt
      781 taattttgct acttttcctc gttggtattc agttgcgcaa taatggcatg accttaaagc
      841 agattgtcct taatcgccgg ggaatgattg tcgccgtggt ggtggttgtc agttcattaa
      901 ttggtggttt aattaacgcc tttattcttg atctccccat caataccgcg ctggcaatgg
      961 cctccggttt cggctggtat tctctttccg gtattttatt gaccgaatct tttggtccgg
     1021 taatcgggag cgcggcgttt tttaatgatc tggcccgtga actgattgct attatgttga
     1081 tccctgggct gattcgccgc agccgctcta ctgcactggg cttatgcggt gccacatcaa
     1141 tggatttcac cctgcccgtt cttcaacgta ctggcgggct ggatatggtc ccggcggcaa
     1201 ttgttcacgg ttttattctt agcctgttag tgccgatcct catcgccttt ttctctgcgt
     1261 aatacctctc tggcggtaga tccctgccgc caaaattgcg ctaaatcaat ctcccttaaa
     1321 gttgcatgaa aaatcccttt tatccccgcg ttaagcgtct taaccttaaa catgtatatt
     1381 aaatataact ttaaaaggtg tgatcatgtt ttgtgtgcaa tgtgaacaaa ctatccgtac
     1441 tccggcagga aacggctgct catacgcgca ggggatgtgt ggtaaaacgg cggaaacttc
     1501 tgaccttcag gatttactca tcgcggcgct gcaagggctt tctgcctggg cggtaaaagc
     1561 gtgtgaatac ggcatcatca accacgatgt tgacagcttc gcgccacgtg cctttttctc
     1621 aaccctgacc aacgttaact tcgattctcc gcgtattgtc ggctacgctc gtgaagcgat
     1681 tgccttgcgc gaggcgctga aagcacaatg cctggctgta gatgccaacg cgcgcgtcga
     1741 taacccgatg gctgacctgc aactggtgag cgatgatctc ggcgagctgc aacgtcaggc
     1801 agcagaattt acccctaaca aagataaagc ggcgattggc gaaaacattc tcggcctgcg
     1861 tctgctgtgc ctgtatggcc tgaaaggtgc ggcggcctat atggaacacg cgcatgttct
     1921 cggtcaatac gacaacgata tttatgccca gtaccataaa atcatggcgt ggctggggac
     1981 ctggcctgcc gatatgaacg cgcttcttga gtgttcaatg gaaatcggcc agatgaactt
     2041 caaagtgatg agcattctgg atgcaggcga aaccggtaaa tacggtcacc cgacgccaac
     2101 ccaggtcaac gtcaaagcga cggcgggtaa atgcattctg atttccggtc acgatctcaa
     2161 agatctctac aacctgctgg aacagaccga aggcacgggc gttaatgtct acacccacgg
     2221 cgaaatgctg cctgcgcatg gctacccgga gctgcgtaaa ttcaagcatc tggtcggtaa
     2281 ctacggcagc ggctggcaga atcagcaagt ggagttcgct cgtttccctg gccccatcgt
     2341 gatgacctcg aactgcatca tcgacccaac cgtaggcgct tatgacgatc gtatctggac
     2401 ccgcagcatt gttggctggc ctggcgtgcg tcatctggac ggtgatgatt tctctgcggt
     2461 tatcacccag gcgcaacaga tggcaggctt cccgtacagc gaaattccgc accttatcac
     2521 cgtgggtttt ggtcgccaga cgctgcttgg cgctgctgat acgctgattg atctggtgag
     2581 ccgtgaaaaa ctgcgtcata tcttcctgct tggtggctgt gacggcgcac gcggcgagcg
     2641 ccactacttc accgatttcg ccaccagcgt gccggatgac tgcctgatcc tgaccctcgc
     2701 ctgtggtaaa tatcgcttta acaaacttga gtttggcgat atcgaaggtc tgccgcgtct
     2761 ggtagatgca ggtcaatgta acgatgctta ctcagcgatt attctggctg tcactctggc
     2821 agagaaactg ggctgtggtg tgaacgatct gccgctgtcg ctggtgctct cctggtttga
     2881 acagaaagcg atcgtcattc tgctgacgct gctttctctg ggcgtgaaaa atatcgtcac
     2941 cggcccgact gcacctggtt tcctgacacc agacctgctg gcggtgctga acgagaaatt
     3001 cggcctgcgt tctatcacca ctgttgaaga agacatgaag caactgttga gcgcgtaagg
     3061 agtttgtcga tgacgatgcc aacgaatcaa tgcccgtggc ggatgcaggt tcatcacatt
     3121 acgcaagaaa cgccggatgt gtggacgatt tccctgattt gccacgatta ctacccatat
     3181 cgcgccgggc aatatgcact ggtcagcgtg cgaaactcag cggaaacgct gcgtgcttac
     3241 accatttcct ccacgccagg cgtgagtgaa tatatcaccc tgaccgtgcg gcggattgat
     3301 gacggtgtcg gctcccagtg gctgacgcgc gatgtaaaac gcggtgatta tctctggctt
     3361 tcggacgcga tgggggaatt tacctgcgac gataaagcag aagataaatt cctgttgctg
     3421 gcggcaggct gcggcgtcac gccgattatg tcgatgcgtc gctggcttgc gaagaaccgt
     3481 ccacaggccg atgtgcgggt gatctacaac gtgcgtacgc cgcaggatgt tattttcgcc
     3541 gatgagtggc gtaactatcc ggtaacgctg gtggcagaaa ataacgttac cgaaggcttt
     3601 atcgctggtc gtctcactcg cgaactgctg gcaggtgtac ctgacttagc ttcacgtacc
     3661 gtgatgacct gcggcccggc tccgtatatg gattgggtag agcaggaagt gaaagcgctc
     3721 ggcgtgacgc gtttctttaa agagaaattc ttcactccag tagcagaagc ggcgaccagc
     3781 ggtctgaaat tcaccaaact gcaaccggca cgagaatttt acgccccggt tggcaccacg
     3841 ctactggagg cgctggaaag caataacgtt ccggttgtcg ctgcctgccg tgcgggtgtt
     3901 tgcggctgct gtaagacaaa agtggtttcc ggtgaatata cggtgagcag tacaatgacg
     3961 ctgaccgacg ccgaaatcgc tgaaggttac gtactggcct gctcctgcca tccgcagggg
     4021 gatttggttc tcgcataatc gccttatgcc cgatgatatt cctttcatcg ggctatttaa
     4081 ccgttagtgc ctcctttctc tcccatccct tccccctccg tcagatgaac taaacttgtt
     4141 accgttatca cattcaggag atggagaacc atgaaacaaa cggttgcagc ttatatcgcc
     4201 aaaacactcg aatcggcagg ggtgaaacgc atctggggag tcacaggcga ctctctgaac
     4261 ggtcttagtg acagtcttaa tcgcatgggc accatcgagt ggatgtccac ccgccacgaa
     4321 gaagtggcgg cctttgccgc tggcgctgaa gcacaactta gcggagaact ggcggtctgc
     4381 gccggatcgt gcggccccgg caacctgcac ttaatcaacg gcctgttcga ttgccaccgc
     4441 aatcacgttc cggtactggc gattgccgct catattccct ccagcgaaat tggcagcggc
     4501 tatttccagg aaacccaccc acaagagcta ttccgcgaat gtagtcacta ttgcgagctg
     4561 gtttccagcc cggagcagat cccacaagta ctggcgattg ccatgcgcaa agcggtgctt
     4621 aaccgtggcg tttcggttgt cgtgttacca ggcgacgtgg cgttaaaacc tgcgccagaa
     4681 ggggcaacca cgcactggta tcatgcgcca caaccagtcg tgacgccgga agaagaagag
     4741 ttacgcaaac tggcgcaact gctgcgttat tccagcaata tcgccctgat gtgtggcagc
     4801 ggctgcgcgg gggcgcataa agagttagtt gagtttgccg ggaaaattaa agcgcctatt
     4861 gttcatgccc tgcgcggtaa agaacatgtc gaatacgata atccgtatga tgttggaatg
     4921 accgggttaa tcggcttctc gtcaggtttc cataccatga tgaacgccga cacgttagtg
     4981 ctactcggca cgcaatttcc ctaccgcgcc ttctacccga ccgatgccaa aatcattcag
     5041 attgatatca acccagccag catcggcgct cacagcaagg tggatatggc actggtcggc
     5101 gatatcaagt cgactctgcg tgcattgctt ccattggtgg aagaaaaagc cgatcgcaag
     5161 tttctggata aagcgctgga agattaccgc gacgcccgca aagggctgga cgatttagct
     5221 aaaccgagcg agaaagccat tcacccgcaa tatctggcgc agcaaattag tcattttgcc
     5281 gccgatgacg ctattttcac ctgtgacgtt ggtacgccaa cggtgtgggc ggcacgttat
     5341 ctaaaaatga acggcaagcg tcgcctgtta ggttcgttta accacggttc gatggctaac
     5401 gccatgccgc aggcgctggg tgcgcaggcg acagagccag aacgtcaggt ggtcgccatg
     5461 tgcggcgatg gcggttttag catgttgatg ggcgatttcc tctcagtagt gcagatgaaa
     5521 ctgccagtga aaattgtcgt ctttaacaac agcgtgctgg gctttgtggc gatggagatg
     5581 aaagctggtg gctatttgac tgacggcacc gaactacacg acacaaactt tgcccgcatt
     5641 gccgaagcgt gcggcattac gggtatccgt gtagaaaaag cgtctgaagt tgatgaagcc
     5701 ctgcaacgcg ccttctccat cgacggtccg gtgctggtgg atgtggtggt cgccaaagaa
     5761 gagttagcca ttccaccgca gatcaaactc gaacaggcca aaggatttag cttgtatatg
     5821 ttgcgcgcaa tcatcagcgg acgcggcgat gaagtgatcg aactggcgaa aacgaactgg
     5881 ctaaggtaaa aagggtggca tttcccgtca taataaggac atgccatgat tgatttacgc
     5941 agtgataccg ttacccgacc gagccgcgcc atgctcgaag caatgatggc cgccccggtt
     6001 ggggacgacg tttacggaga cgaccctacc gttaatgctc tgcaggacta cgccgcagag
     6061 ctttccggta aagaagccgc catttttctg ccgaccggca cccaggctaa cctggtcgct
     6121 ctgctcagtc actgcgaacg cggcgaagag tatattgtcg gtcaggccgc gcataattat
     6181 ctgtttgaag ccggtggcgc agcggtgctg ggcagtattc aaccgcaacc catcgacgcg
     6241 gctgccgacg gcacgctacc gctggataaa gtggcgatga aaatcaaacc cgacgatatc
     6301 catttcgccc gcaccaaatt actcagtcta gaaaacaccc acaacggcaa agtgctgccg
     6361 cgtgaatacc tgaaagaagc atgggaattt acccgcaagc gcaatctggc gctgcatgtt
     6421 gacggtgcgc gcatctttaa tgccgtggtg gcttacggct gcgaactgaa agagatcacg
     6481 caatattgtg attcgttcac catttgcctg tcgaaaggtc ttgggacgcc agtcggttca
     6541 ttactcgtcg gtaatcgtga ttacattaaa cgtgccatcc gctggcggaa aatggccggt
     6601 ggcgggatgc gtcagtccgg gattctggct gccgccggga tgtatgccct gaaaaataac
     6661 gtcgcacggt tgcaggaaga tcacgacaac gccgcctgga tggcggagca actgcgtgaa
     6721 gcaggcgcgg atgtgatgcg tcaggacact aatatgctgt ttgttcgcgt cggggaagaa
     6781 aatgctgccg cgttaggcga atacatgaaa gcgagaaacg tgctgattaa cgcctcgccg
     6841 attgtccgcc tggtgacgca tcttgacgtc tcgcgcgaac aactggcgga agtcgccgcc
     6901 cactggcgcg cattcctggc gcgttaagga gagagacgtg ccgcaacgca ttttagttct
     6961 cggtgccagt ggctacattg gtcagcatct ggtgcgcaca ctcagccagc aagggcatca
     7021 gatcctggcg gcggcacgtc atgtcgacag gcttgcaaag ctgcaactgg caaacgtcag
     7081 ttgccataaa gtcgatctca gctggccgga taaccttccg gccctgttgc aggatatcga
     7141 taccgtctat tttctggtgc acagcatggg cgaaggcggc gattttatcg ctcaggagcg
     7201 ccaggtggct ctcaacgtcc gcgatgcgct acgtgaagta ccagttaagc aattaatctt
     7261 tctcagttcg ttgcaggccc cgccacatga gcagtcggat catctgcgtg ctcgtcaggc
     7321 tacggcggac attcttcgtg aagcgaatgt acctgtgacc gaacttcggg ccggaattat
     7381 cgttggcgca ggttcagcgg cgttcgaagt catgcgcgat atggtctaca acctgccagt
     7441 gttaacgccg ccacgctggg tacgttcacg caccacgccc atcgcgctgg aaaacttgct
     7501 gcactatctg gtggcgttgt tagatcatcc ggccagcgaa caccgcatct tcgaagccgc
     7561 cggaccagag gtgctcagtt atcagcaaca gtttgaacat tttatggcgg tgagcggtaa
     7621 gcgccgctgg ttgatcccca tccccctccc cacccgctgg atttcggtgt ggtttctcaa
     7681 tgtgattact tccgtaccgc ccaccaccgc cagggcgttg attcaggggc tgaaacacga
     7741 tctgctggcg gatgataccg cgctacgtgc actcatccca caacggctga tcgctttcga
     7801 tgacgcggta cgtagcacgt tgaaagagga ggaaaaactg gtcaactcca gcgactgggg
     7861 ctacgacgct caggcctttg cccgctggcg accggagtac ggttattttg ccaaacaggc
     7921 ggggtttacc gttaaaacgt ccgccagcct tgctgcttta tggcaggtag tgaaccaaat
     7981 cggcggtaaa gagcgttatt tctttggcaa tattttgtgg cagacacggg cgttgatgga
     8041 ccgcgcgatc ggtcataagc tggcgaaagg ccgcccggag cgcgaatatt tacagactgg
     8101 cgatgcggtg gatagctgga aagtgattgt cgttgaaccg gaaaaacaac ttacgttgtt
     8161 atttggcatg aaagcgtcgg ggctgggacg actgtgtttt agcctggaag ataaaggcga
     8221 ctatcgtact atcgatgtcc gcgctttctg gcatccgcac ggtatgccgg ggctgtttta
     8281 ctggttattg atgatccccg cgcatctgtt tatttttcgc ggaatggcaa aacaaatcgc
     8341 cagactggca gaacaaagca cagattaatc gacaaaacct gcgttattct ttcatgtttc
     8401 ccattgcatc atgggggaaa tcacggaaga atgcgcacgt cattttctca acaacagtgg
     8461 attgatatga aggtactggt taccggcgcc accagcggct taggtcgaaa cgcggtagag
     8521 tttttatgcc agaaaggcat cagcgtgcga gcgaccggtc gcaacgaggc aatgggcaaa
     8581 ttgctggaaa aaatgggcgc agagtttgtt ccggcggatc tgaccgagct ggtttcatca
     8641 caagctaaag tgatgctcgc gggcattgat acgctgtggc actgctccag ctttacctca
     8701 ccctggggga cacaacaggc tttcgatctg gctaacgttc gcgccactcg ccgcctgggt
     8761 gaatgggctg tcgcctgggg tgtgcgtaac tttattcata tctcttcccc ctccctgtac
     8821 ttcgattatc accaccatcg cgatattaaa gaagattttc gccctcaccg cttcgccaac
     8881 gagtttgccc gcagcaaagc agccagcgaa gaagtgatca atatgctttc gcaggcgaat
     8941 ccacaaacgc gctttactat tctgcgccca caaagtctgt tcggaccgca cgataaagtc
     9001 tttattcccc gtctggcgca tatgatgcac cactacggca gtattctgtt accgcatggc
     9061 ggcagtgcgc tggtcgatat gacctactat gaaaataccg tgcacgcaat gtggctggca
     9121 agccaggaag cctgcgataa gctaccttcc gggcgtgtgt acaatatcac caacggcgag
     9181 catcgcacac tgcgcagcat cgtgcagaag ctgatcgacg agttgaatat tgactgtcgt
     9241 attcgttccg tcccctaccc gatgctggat atgatcgccc gcagcatgga gcgtttaggc
     9301 cgcaagtcag caaaagagcc gccgctgacc cactacggcg tctccaagtt gaattttgac
     9361 tttacgctgg atattacgcg tgcgcaggaa gagttaggtt atcagccgat catcacccta
     9421 gatgaaggta tcgagaaaac tgccgcctgg ctacgcgacc acggaaaact gccgcgctaa
     9481 tcctgcccgt atttctccag caatgcttcg gcaatcgcct gagtttctgc atccgcttcg
     9541 ccgttatata acgtcgggcg gaaatgcatc tggaatgcca taatcacgcg ccgctgctcg
     9601 cgcggtgtca tatcaggttt aacgtcataa ccgtagcgcg ccaaaagctc cagcaatgac
     9661 gcggtatcta ccggtgtgtg cggcgcgcgc ccggcaaggt aaaagttaac ccgctgcgca
     9721 tccggccagg caccaatccc ctgctgcgcc agttgctgcc agggaaataa tggccccgga
     9781 tcgtctttgc gctgcggtgc gatatccgca tgtgccacta cgttttccgg cttgatgtga
     9841 taacgggcaa taatatcttt cgccagtgga ataagcgcct gaatctgtgt cggttcaaac
     9901 ggggcaaaat atttcactcc ggccgatttt tgccagccac ggttttccag ctcaatgcca
     9961 atagaggtgt cgttaaggcg cgttgccccg cgccaggcgc taatccccgc atgccaggcc
    10021 agttcttgtt ccggcaccag ttgccagatg cgcggtttac cgttgtatcg cggtggtacc
    10081 gcagggacca gataatgcga gctgacctgc ttatcggtca gtgtcgccag cgagctatca
    10141 aaatcatctg cggtgtagtg gatcaccagc actttaatgc gcggatacgc cgcctgcgcc
    10201 tggcgtcggg tatcaagctg atatccctct ttctcgacaa tgcctttttc gcctgcacac
    10261 cctgccaata acagagcggc agcgaccagc cagaagactc ttctcatcga cgcgttttca
    10321 ccgccgtacc gctgacgcta accatcagca tactgccgtt ttgcccgacc gtttcgtagt
    10381 cgatatcaat accgacgacg gcatcggccc ccagcgcccg cgcctgggag cctaattcct
    10441 caaaggcgat ctcccgtgct ttacgcagtt ctttttcata cgcaccggaa cgtccgccaa
    10501 cgatatcgcg gataccggca aagaaatcac ggaaaatatt ggcacctaaa atcgcttcgc
    10561 cggtcaccac accgcaatat tcaacgatgg tctggccttc cagagttggg gttgtagaaa
    10621 attgcatgat cgatctcctt gtttgttaag ccttcgatct ctaaagcatt atcgaactga
    10681 tatgccatgt tgggaagaag gttttgatca gtttaggaaa tcatctcatt gtgtcaaaaa
    10741 cacccactta agtctttacg attatccgtt gcataacccg atattggttt cattataggg
    10801 taccgcttac ctgtcttatt atgttgcacc aacaacatta atgagcggaa agaggcaact
    10861 tatattgtaa atgactccta aagtcttccc catcttgtga ctgaggtata tacctctgga
    10921 ttgctttttg gtcagacgcc gcaaaacaag aaagtaataa gatctcaatg cttttgtcat
    10981 taatataagc gaagatcatt cttgcaaatt cgtaaataaa cgcccgttcc tgagcgctta
    11041 atagtttaat gcaatattta ttcttaagag agaaaataat ttcaaggaaa gcatccgcag
    11101 caaccttcac gttattcccg gcgctcatat tatatatatc ccttgcacag atagagtaca
    11161 gagatagtaa acagccatac aaataatcat ttattaactc ttttgtccat tttactggca
    11221 accatggttt tatcttgaca ccatgcatac tacagtcatg aaaaagacat ttgctgaaat
    11281 taactcttat catatcaaca ttaaaaaaat ggcaattaat aaattttaaa gaatgagtat
    11341 ttacgttgtt aattttaaca gatctaaacg aaacattttt gaatacatta tttattatat
    11401 tgctatttgt tattttaaca tcacctaaac tgccgttttc aaaacaacat tttttcaata
    11461 tgctttcatt cacaacagtg tgtgataagt agagattact tatgttttgc tttattaatg
    11521 ccacgtcatt aaaatgacta tatttaatga aaaaaccatc attgagtagt gttttaagat
    11581 tgactaaatt acttgcatct tcacgtacca tcgtggcaac tggaacaggc agagacatga
    11641 aaacttcttc attcaccatc aagtgaatca tgcattgcgt tccctttgtc atatccacac
    11701 atacgctaca aatatcgtgg taatgcgaag agcgcatttc aggaaacaga ttaaacacgg
    11761 tatcttgaaa ggcactaaat cgtgttaata aatcctgata atgaacaggt tttgtttcaa
    11821 aattatcatc agcaaatcca agcactattg tatgctctac atctaacgca gtttcaaatg
    11881 gctcttcttc tgtaaaatca ttatttaaaa cctcacctga cgtattatca ctgtcataaa
    11941 aagtgttcat caatgcgata ttgtttgtta cagcgtcttt ttcgatattc tcactaattg
    12001 catatcgcct gctataatca taaaaatgaa taaacgcgcc ctttataaag gatatatatt
    12061 gttctttgtt aaagtcaata cggctgtata atttttcaaa aatcacattc gcaattttac
    12121 taccaatggt atgcctgtat aataatgggt gcgattcaat cgtaaaatgc ttgtctaatt
    12181 gatcttgtaa tttattctca tagtccagag ggaaaagaaa ctgttgtctt tcaagtttcc
    12241 cattcgatac attacgtatt ccaatagaga ttctttcatt acccgccttt atttcaactt
    12301 cgttatcttc tgatttaccc agatattttt taacatcagt ctcgaaagca ttactaaaaa
    12361 aagtattaaa ttggtaattc gctgatggca tattttccgc gagcaacaca tcatttttta
    12421 tgagatcact gtcagcattt attgctaata tcagatgcaa gtagatggta tcccatgtag
    12481 acaactcacc cttacgactg gaatttaata tcgcgaaaaa ttccctcagt gaatccagga
    12541 aattatcact ttgatttagt tgtgtgcctg gaaatgatga tgcttgatca gatatattgt
    12601 ttatcatatt aaaaatcacc caagaaaaac gataatccac cgaactcctc gtgctttata
    12661 attataaaag cacaacgcct tttatattca tattaattaa cgagatactg aaacaactta
    12721 atttattctt cgctaaaatt cattagctta aattctttag ccacaattgt agcataaata
    12781 atattatcga gagtcaaagc tgggactatc aaatttcgct cattttcaaa aatacaacgt
    12841 aattttggca atcatcttaa tcgcaggatc tcttatcgca tgtgaaaaat tttgtaatga
    12901 ttagacaata accgcgttca tttatcacgc gttataaatg tgaaactggc ttcttgcaga
    12961 tgctcttaac ctctacagga ggatttatac caccatttca gaactatcat atttacctta
    13021 aatgatcatt tcagccatat aacacgctca acctcacaaa tggtttacac tgtctattgt
    13081 ttgggtaatt taagtaagtc agctacgagc aaccttgagt ggatagccag caatggactc
    13141 aaacccaatc aggtgttcag attgtttcag aattgtatta aaaacaatgt attaaataat
    13201 ctgatagcgt ttggtcgtag tgtgacgcag acagcaagct aataaggaaa tgattatgcg
    13261 ctaccgttca ctatctctac ttattccatg tgcactgctg ctgagtgcct gtaccaccgt
    13321 cacgccagct tataaagata acggtccccg caccggttcc tgtgtccagg gggggcctga
    13381 cagcgtggct caacaattct atgactaccg cattcaacat cgcagcaatg acattaccgc
    13441 cctgcgcccc tatttgagtg acaaactggc aacccttctt agcgatgcca gccgcgataa
    13501 tagtcatcgt gaattgttaa gcagcgatcc gttctccagt cgaaccacct tgccagatag
    13561 cgcccacgtt gccagtgcat cgactatccc taatcgtgat gcccgtaaca ttccattgcg
    13621 cgtagatctc aaacagggtg accagggttg gcaggatgaa gtgttaatga ttcaggaagg
    13681 ccagtgttgg gtcatcgacg atgtgcgcta cttaggcggc agcgttcatg ccacggcggg
    13741 tacgctccgt cagtccatcg agaatcgcta acgccttgat caaccaataa accccgtaaa
    13801 ttgtccggca tttcgtgagg aatgccgaca tttatgctcg ccgaccaccg cccccgttat
    13861 tttgtgctat gtttattgaa taatgcgctt tgcttttaac ttttaaagca gaaatattgc
    13921 ataattattc tgtcaaaggt actatcttcg acctcaatag ctatcagact gccagtatac
    13981 gagtgtcaat gagtattcaa ttaaacggca ttaattgctt ctacggcgcg catcaggcgc
    14041 tgttcgatat cacgctggat tgcccacagg gcgaaacgct ggtgttactt ggccccagcg
    14101 gcgcgggtaa aagctcgctg ctgcgtgtac tcaatctgct tgagatgcca cgctccggta
    14161 cgctcaacat tgcaggcaac catttcgatt tcaccaaaac accctctgac aaagcgattc
    14221 gcgatttgcg tcgtaacgtt ggcatggtgt ttcagcaata caacctgtgg ccgcatctga
    14281 ccgtgcagca aaacctgatt gaagcgccct gccgtgtact ggggttgagt aaagatcagg
    14341 cgctggcccg tgcagaaaaa ctgctggaac gtctgcgtct caaaccttat agcgatcgtt
    14401 acccgctgca tctttctggt ggtcagcagc agcgtgttgc tattgcccgt gcgttgatga
    14461 tggaaccgca ggtactgctg ttcgatgaac cgaccgccgc actggacccg gaaattacgg
    14521 cacaaatcgt cagcatcatt cgtgagctgg cagaaacgaa tattacccag gtgatcgtca
    14581 cccacgaagt tgaagtggcg cgtaaaaccg ccagccgagt ggtgtatatg gaaaatggtc
    14641 atatcgtaga acaaggcgac gcgagctgct ttaccgagcc gcaaaccgaa gcatttaaaa
    14701 actatctctc tcactaagat tcgggataac aacaatgaaa aaagttctga ttgccgcgtt
    14761 aattgcaggt tttagtcttt ccgccacagc tgccgaaacc attcgttttg ctaccgaagc
    14821 atcctatcct ccgtttgaat cgattgatgc aaacaaccag atcgttggtt ttgacgtcga
    14881 cctggcacaa gcgctgtgta aagagattga tgcaacctgc actttctcta accaggcgtt
    14941 tgacagcctg atcccaagcc tgaaattccg tcgcgtagaa gccgtgatgg cgggcatgga
    15001 tatcactccg gagcgtgaaa agcaggtgct gtttaccacc ccgtactatg acaactctgc
    15061 cctgtttgtg ggtcagcaag gcaaatacac cagtgttgat cagctgaaag gcaaaaaagt
    15121 cggcgtacag aacgggacga cacaccagaa attcattatg gataagcacc cggaaatcac
    15181 taccgttccg tatgacagct accagaacgc aaaactggat ctgcaaaacg ggcgtatcga
    15241 cggcgtcttc ggtgacaccg cagtggtcac tgagtggctg aaagataacc cgaaactggc
    15301 ggcggtgggc gacaaagtga ccgataaaga ttacttcggc actggcctcg gcatcgcggt
    15361 acgtcagggc aacactgagc tgcagcagaa actcaacact gcgctggaaa aagtgaagaa
    15421 agatggcact tacgaaacca tctacaacaa atggttccag aagtaattcc tgatgaatga
    15481 attttttcct ttagcaagcg ccgccgggat gaccgtcggc cttgccgttt gtgcattgat
    15541 tgtcgggctg gcgctggcga tgttctttgc ggtatgggag tcggcaaaat ggcgtcctgt
    15601 cgcgtgggca ggttcagcgc tggtaaccat tctgcgtggc ctgccagaaa ttctggtggt
    15661 gctgtttatc tattttggct cctcgcagct gctgctgacg ctttcggatg gcttcactat
    15721 caatcttggg ttcgtgcaga tcccagtgca gatggacatt gagaacttcg acgttagccc
    15781 gttcctttgt ggtgtcatcg ctctgtcact gctgtatgcc gcctatgcct cgcaaacgct
    15841 tcggggcgcg ttgaaagcgg tgccggtggg tcagtgggag tctggtcagg cgctggggct
    15901 gtcgaaatcg gctatctttt tccgtctggt gatgccgcag atgtggcgtc atgcgctgcc
    15961 tggcctcggt aaccagtggc tggtgctgct gaaagatacc gcgctggtca gtttgattag
    16021 tgtgaatgat ttaatgctgc aaacaaaaag catcgctact cgtacccagg aaccatttac
    16081 ctggtacatt gtggcggcgg cgatttacct ggtgatcacc ctgctcagtc agtacattct
    16141 caaacgcatt gacctgcgcg cgacacgttt tgagcggagg cccagctaat gtttgagtat
    16201 ttacccgaac tgatgaaagg gctacacacc agcctgacgc taaccgttgc ctcgctgatt
    16261 gtggcactga ttctggcatt gatttttacc atcatcctga cgctgaaaac gccggtgctg
    16321 gtgtggctgg tgcggggtta tatcacgctg tttaccggta cgccgctgct ggtgcagatc
    16381 ttcctgattt attacgggcc gggccagttt ccgacgttgc aggagtatcc ggcactgtgg
    16441 catttgttgt cagaaccgtg gttatgtgcg ctgattgcgt tatcgctgaa cagtgcggcg
    16501 tataccacgc agctgtttta cggtgcaatt cgtgcgatcc cagaaggtca gtggcagtcc
    16561 tgtagcgccc tgggaatgag caaaaaagat acgctggcgc tcctgctgcc gtatgccttt
    16621 aaacgctcgc tctcttctta ttccaacgaa gtggtgctgg tgttcaaaag tacctctctg
    16681 gcttacacca ttacgctgat ggaagtgatg ggatacagcc agttgttgta cggacgcacc
    16741 tacgatgtaa tggtgttcgg tgcggcaggg attatttacc tggtcgtcaa cggcctgctg
    16801 acgctgatga tgcgtctgat cgagcgcaaa gcactggcat tcgaacggcg gaattaatgt
    16861 agctgcgtac attcgccggg ggggtttgta ggtctgataa gacgcgccag cgtcgcatca
    16921 gacatcagca cggtgcctgt gccggatgcg gcgtggacgc cttatccggc ctacatagtg
    16981 cataaattct tataatgaag acggacaact cactaagtta tccgtctttt tttatttcat
    17041 ttaaattatt taatcatatt tattgcatat aaattcactt gatggcattg ttatcccatg
    17101 ccgcagacac ggccaaaaat cataagattg acagacggga gttccatcat gaaaaagtta
    17161 gttcttgccg ctttacttgc ttcctttact ttcggtgctt ctgccgcaga gaaaatcaat
    17221 tttggcgttt cagccaccta tccacccttt gaatctatag gtgctaataa tgagattgtc
    17281 ggctttgata tcgatctggc aaaagccttg tgcaaacaaa tgcaggcaga atgtactttt
    17341 actaatcacg cgttcgacag cctgatcccg tcactgaaat tcagaaaata tgactccgta
    17401 atctccggta tggatatcac gcccgagcgt agcaagcagg tatcgtttac cacgccctac
    17461 tatgaaaact cagccgtcgt gattgccaaa aaagatacct acaaaacgtt tgccgatctg
    17521 aaaggcaaac gtattgggat ggaaaacggc accacgcacc agaaatatat tcaggatcag
    17581 caccccgaag tgaaaactgt ctcctatgac agttatcaga atgcctttat cgatctgaaa
    17641 aatggtcgta ttgatggggt atttggtgac acagcggtgg taaacgaatg gctgaaaacc
    17701 aatccgcaac tgggcgttgc tactgaaaaa gtgaccgatc cgcaatactt cggcaccggt
    17761 ctgggcatcg ctgtacgtcc ggataacaaa gctctgctgg aaaaactgaa taacgcgctg
    17821 gcagcaatta aagctgacgg tacatatcag aaaatcaatg accagtggtt cccgcagtaa
    17881 ttgagactga tgacaaaagt aagattgcct gatgcgctgc gcttatctgg cccacatcaa
    17941 ctctgcaatg tattgattta gcatgatttt gtaggccgga taaggcgtac acgccgcatc
    18001 cggcatgaac aaagcgcacc tgatcagcaa tctgaagccg cacctgcggc ttttttattg
    18061 cttcaccagc agcgtcatca cttcatagtg cgcggtgtgc gggaacatat caaaaagctg
    18121 tacccgttcg atgcggtaac ctggcagttc gcggatatct ttcgccatgg tttgggcgtt
    18181 acagctggag tagataataa aacgcggtgc catcgttgag agataatcac acagcggttt
    18241 accaatgccg cggcgcggcg ggttaaccag caccagctcc ggcacatccc cctgagcggt
    18301 ggcaaactga gtggagtcca gcgcctgaaa ttgcaaacgc gttaagccca gttcagcggc
    18361 tgactgcttt gcacaggcaa tggcctctga tgcaatttcg atcccggtta actgcatgtc
    18421 aggcgtcgcg cagtgtaaac caaagccacc cacaccgcag aacagatccc acatatgttt
    18481 aactggcagc tgccgtaccc agtcgcgcgc ggtagcgtac agctggctgg cgaccgccgg
    18541 attagtctgg aagaaacttt gcggacggat ccacagcggt acgtcattaa aacgctctgc
    18601 cagcgcctgt tgttcagtca ggtagatctc cgtctcccct tccataatcg ccatatgtac
    18661 cggctgaata ttgacggtaa taactttcag ttgcggtagt tgttcctgta accacggcag
    18721 cgccttacgc agttgtgcca gtttggtttc agaacgcagt acaaagcgca gcatcatgcc
    18781 gccatcgctc tggctttcag tcagcagaat gtatttcagt tcgccacgct tacgcgccac
    18841 gttgtagggc gttaaccccg cacgggcgat aaacggtttt agcgccgcaa aaacgggcgc
    18901 aaaagaggca ggataaagcg ggcagtcaca aaggtcttcc ggcgtgccat cgcgatgcag
    18961 cataccgagc agtggttttt caacgctacc actcaccacc attttggctt tattacgaaa
    19021 cccttgttcc gggcctgaca ccggcgcgca ccattcctca accggaaaat cggcgagcag
    19081 atttttaaga tcggcggttt tagcggagag ttgctctgga atcggctgcg ttatccactg
    19141 acaggaacga cagcgacccg cgtcgtaaag tgcgcactgc atacattgac cttcacatca
    19201 tcaggggcga cgattataca cattattgca actggaagaa ccgccgactg gtcgagggaa
    19261 cgaacagcag catcaggatg agcatatccg gcagcttctg cagcatcagg ctatggaaga
    19321 tttcacgttt tgattcaccg ggaatgctga acagctccgg ataaccgtac cccagcgaag
    19381 ccgcccacaa gtaacttgcg gcggtgattt gtgtcagcag atatagccag cgcgcccagc
    19441 gacgcccttt caccagtgaa aacgcacacc agatctcaat gaaaaccagc accagactgc
    19501 ttaaaaagac cagcgttaaa ctccaggttt gtacgctgcg atgaatgaac tcgccaatac
    19561 cgcgcacgcc cagcgtattg aaaatcatca gcacgtcgag gccacggatc ataataatgg
    19621 cgagcgccgc cacctgcacc agcgcaggca cattcaggcg agcatgagat gaagatgttt
    19681 tcttaaaaaa tcccaacgtt tcgtcttcca tgaaaacaat gccgcggcat gcgcggcatt
    19741 atgtagccag gttggcaaat tttagtgtct tcagccatgt cttgcacgct ggatatcgcg
    19801 tatccgctgt ttttctgcgc gagccatcag ataccaggcg ataaatccga caattccgac
    19861 cgcaccgaga attaacgttg caagggcgtt gatttccgga ttcactccca tccgcacgct
    19921 ggaaaagacc agcatcggta acgtggtggc tcccggcccg gaaacaaagc tggcgatcac
    19981 cagatcatca agcgacaaag taaaagccag taaccagcca gaaatgatcg cgggcatgat
    20041 catcggtagc gtaatgacga aaaacacttt cagcggcgtc gcaccgagat ccatcgctgc
    20101 ttcttctatc gagcgatcca gttcccgcaa tcttgaagaa ataacgaccg ccacataagc
    20161 cgtacaaaaa gtgacatgcg ccagccagat ggtgagcata ccgcggtccg caggccagcc
    20221 aatggcatga gcaagcgcga cgaataataa caacagcgac aagcccgtga tgccatctgg
    20281 catcaccagc ggcgcggtga tcataaaggc aaaaccattt gatccgcgaa acctgccaaa
    20341 acgcaccagc accaccgccg caatagtccc gaggatcgcc gccgccgttg ccgcacaggc
    20401 agcaattgtc aggcttaaac caaccgcact catcatcgcg tcatcgcgca ataactcacc
    20461 ataccagcgc gttgaccagc cggcccacac cgtcaccagc ttcgagctgt taaacgaata
    20521 gataaccagc atcagcattg gcgcgtagag aaaggtaaag cccagcagca aaatcacaat
    20581 ccgccagggc gaacgaacta ccggtaaatt attcatccgt gttctcccac gcttttttgc
    20641 tggtgtttgt ggaaccacat tatcggcaca attagcagca gcaacatgat gatcgctacc
    20701 gccgaggcca ccggccagtc gcggttattg aaaaactctt gccataacac gcgcccgatc
    20761 atgatgctgt ccgggccacc gagcagttcc gggatcacaa actcgcccac agccgggata
    20821 aacaccagca tcgatccggc aataatccca cctttagtca gcggcacgat cacagtaaag
    20881 aacgttttca gcggtcgtgc accgagatcc agcgctgctt ccaccagcga ataatcaata
    20941 cgaatcaacg cggtataaat cggcagtacc ataaacggca cgtaagcgta aacaatgccg
    21001 atataaacgg ccagattggt atgcagaatg gtcagcggtt gatcgataac ccccagccac
    21061 agcagaaaat tattcagcac accgttgttt tttaatattc ccatccaggc ataaacgcgg
    21121 atcagaaacg aggtccacga cggcaggatc accagtagta ataaaatatt acgggtcgaa
    21181 ggcttactgt gcgccaccgc ccacgccagc ggatagccga tcagtaaaca gcaaaatgtc
    21241 gaaatcgccg ccacctgtaa cgactggaga taagcatcaa aatagagcgg atcgtcggtc
    21301 agttgcagaa aattaccgag attaagagtg atggaaagtt gcccgtcagc ccactccatc
    21361 agttcggtat aaggtggaat agcgcgcgcc atctctccca ggcttatttt aaagacaatc
    21421 agaaatggca gcagaaacag caagatcaac cagatatata gcaacgcaat gaccagtttg
    21481 cgcccatgct tcatttgcag ctgcgacaac cacagcttaa atccgcccgg cggtttcgac
    21541 tgggcagcag gttcaagtgt actcatcttt gctccttaaa ccgtcagcac cacacagctg
    21601 tccacttccc agcacaaacg cacttcgtcg ccccaggtcg gtaacccttt acgatgacga
    21661 tgggcgtttt gtagctgggc gctgatcatc tgcccacttt tcagtcgaac gtgatacacc
    21721 gaaagatcgc cgagataggc aatgtgtatc acctccccca ccgcgaagtt acaaccattg
    21781 gcgggcggct cttcgcaaag catgattttt tccgggcgca gcgctacgtg taccggcacg
    21841 ttatcgacca ctgaagcatc cgcatcgact ttcagtggat gcaccagccc cggcgaatca
    21901 agcaccaggc catcttcctg acgctctttg agtacacctt caaagacatt aaccgaaccg
    21961 ataaattcag cgctgtagcg ggtagttgga tgctcgtaga tctcttccgg ctcgccaatc
    22021 tggacaaatt tcccgcgatt cataatggcg atacgccccg ccatggtcat cgcctcttcc
    22081 tgatcgtggg tgaccatcac acaagtcaca ccgacgcgct ccagaatatc caccacttca
    22141 agctgcatcc ggtcacgcag ctttttatcc agcgcgccca tcggctcatc gagcagtaat
    22201 agtttcgggc gtttggcaag gcttcgggcc agggccacac gctgtcgctg accaccggaa
    22261 agctgatgcg gtttgcgttt ggcgaactcc tgcatgtgca ccaggccgag catctcatgg
    22321 acccgactgg caatttccgc tttcggtagt ttgtcctgtt tcaggccaaa agcgatgttc
    22381 tgttccacgg tcatatgcgg aaacagcgcg taagactgaa acatcatatt gatggggcgc
    22441 aggtaaggcg gcacctgtga caaatcgacg ccatcgagca ttatctgccc ggcagaaggt
    22501 tgttcaaaac ctgccagcat acgcagcagc gtggacttgc cacagccgga tgcgcccagc
    22561 agcgcgaaga tttcaccttt gtagatggtc aggctgacat catcgaccgc atgctgacca
    22621 tcgtaggatt tggtgaggtt gcggatttct aatagcggcg tcagcgcctt acgggttttc
    22681 gcctgcgggc gagggatagc gtcattcacg gctgctctcc ggcattgaat gtgtgcaagc
    22741 gcaccatgat ggtgcgaatt gccggctggt gtggtgcgcc cgcccggcat ctaaggctgc
    22801 ggattatttt ccgctcttca ctttggtcca cgcgcgggtg cgcacacggt cgattttcgg
    22861 atcctgtact ttcagagtga atagcttcgc acgaacgtcc gccggaggat aaatacccgg
    22921 gttctcacgg acttccgcac tcaccagcgg cgtggctgct ttattggcgt tggcatagaa
    22981 cacatggtcg gaaatatgcg ctactacatc cgggcgcagc aggtaattca ggaactgata
    23041 ggcttcgtct ttgtttttgg catccgcagg catggcgaat acatcaaaga acgccatcgc
    23101 cccttctttt ggaatcgaga acgagacatt cactccattc ttcgcttcct tcgcgcggtt
    23161 tgacgcctgc cagacatcac ctgcccagcc gatagcgacg caaatatcgc cgtttgccag
    23221 gtcgttaatg tattgagatg aatggaaata acgaatgttc gggcgcagct ttaacagcag
    23281 atcggttgcc ggtccggtgt aatcatccgc tttagtgctg ttgggatctt tgccgagata
    23341 attcaacacg gtagcaaaaa cttcttctgg cgcatccagg aaagagacac cgcagctttt
    23401 cagtttttcc agattttcag gtttgaggat caagtcccag ctatcgacgg gcgcgttttc
    23461 gcccagcaca gctttaactt tatcaacgtt atagccaatc ccggtcgtcg cccacatata
    23521 gggcatagca aatttattgt cgggatcgtg tttggcgacc agcttcagca gctccgggtc
    23581 gagattcttc cactccggca atttgctttt gtccagcggc tggaaaactc ccgcagtcaa
    23641 ctggcgttca agaaagctgg cagatggaac caccaagtca aagccggtgc tacccgccat
    23701 taatttgcct tccagtactt cgttagagtc gaaaacatcg tagacgactt taataccggt
    23761 ttctttttca aaattggcca ccgtgtccgg ggcgatataa tcagaccagt tataaatgtg
    23821 gagtgttttt tgttcagccg cgagcgtgcc gacagagacg gccatcagag cacccgcagc
    23881 cagacccgaa agccattttt tatttaaggc ggtcatatct ctttccttct gaaagttcgt
    23941 taacaaacga acaaaagttg tcacactaaa agatatgcaa taaacgtgca tattttttat
    24001 cggcggggtg taagaaaaga gaactctctc ccggccaaca ctgctcgcta tataaaagca
    24061 tcgcaagaat aactttagcc agggtttgag actatagctc ggattaaaaa acggtattta
    24121 acaaattttt atgcgtattt gctctatgtg ataaagccaa atcgacacaa gagaagtgaa
    24181 ataatcttta agaaaaggtt aaaaacagca gataattttg cagcgcgcag ccactgcacc
    24241 agcgactgcg tgctcgaaag gagttatcag tggaggaaat agttttcggt aacgttgttt
    24301 tgcccttcat cgtcagtcgg taacagcagt tgatgggcat tagcttcaag aatgaccatc
    24361 gaaatctgct cttcgctctg acgcacgaac catgcaaact gctcataggt tacgccctgc
    24421 ataacggata aagactggca aaccaccagc tttggcagat tatcatcctg catatcaaga
    24481 aatgctttca cggtcagcga actggcattg atggcagata aatccgccgc cagcggcaat
    24541 acggctgaag gtcggacttc cgccatggca gaaaacagga tcgtgttatc gatcagatcg
    24601 attttggcat caaagacacc gtcgaaattc tgcatatggg gcagatgcag agcctgacag
    24661 ttatcacatt caaaaaaact catccccagg tcatcgagcc attgacgcag cgtatccaga
    24721 ccaggaacga ccagcgatgt cataattttg tacaacctct ttcgataaaa agaccggcac
    24781 agcttacgca aaaagcgcag gcaaaaccat gatcagtaat gtgattgcga ctaaccaccc
    24841 gttttcaggc aatattctgt cgtagcgtgg cgttcgatcc agcggatcat tttacccgcg
    24901 atgtcgatac cagtggtttt ttctattcct tccagccccg gcgacgcatt cacttccatc
    24961 accaacggcc cgcgattagc acgcagaata tccacaccag caacgtccag cgccatcgtt
    25021 cgcgcggctt ttatcgcgat ttcacgctcc tgtggtgtga tactggcgac gcttgccgcg
    25081 ccgccacgat gcaaattgga acgaaaatcg ccctctttcg cccgccgttc aatcgcagcg
    25141 accacttcat cgccaacaac cagacagcgg atatcgcacc cttgcgcctc tttgatatat
    25201 tcctgcacca gaatatgcgc gttcagaccg cggaaagcgt caatcacgct ttccgccgcc
    25261 tgacgcgtct ccgccagcac gacgccaatt ccctgcgtgc cttcaaccaa cttgaccacc
    25321 agcggcgcac caccgaccat gtcgattaaa tcgctggtat catccggcga atgcgcaatg
    25381 cccgtgacag gcaggtcgat gccctgacgc gccagcagtt gcatggaacg caatttgtca
    25441 cgcgcccggg caatggcgac cgactcattg agcggatagc tccccagcat ctcgaactgg
    25501 cgcagtgccg ccgtcccata aaaggtaatg gcggtgccaa tacgcgggat cactgcgtca
    25561 aaatggggta acttgcggcc tttgtagtga atagaagacg ccgcaggatt tatgttcatg
    25621 tagcaagaaa gcggatcaag aatttcaacc aggtgaccgc gctgtatagc ggcttcacgc
    25681 agccgcttac acgaatagag cgttccatcc cgggacaata tggcaatttt caccctgcac
    25741 ctctctgtca gacctggtca aaagcccgcg tatcatacat cgacgtggcg gttttagcgc
    25801 gtcgcccaac cctgtttgtg caaataatcc agaataaatg ggcggctttc tttaatgatt
    25861 gttcggcgga tatgatcgct ccaggtatcc cggcgattat tgctgccacg ggtgaggtaa
    25921 tattccgcca gttgctcgtc atactgcgcc agtgcgtctt tatccagcgg ttgatagctg
    25981 ttttcatgca ccaaaatgga ggccggtaaa cgcggcttga gatccggatt atccgcaggc
    26041 cagccaaggc acagcccaaa cagcggcaga acatgctgcg gtaatttcag cagtttcgtc
    26101 accgcttcaa tattattgcg caggccgccg atatataccc cg
//
LOCUS       DAJVQG010000044        26013 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJVQG010000044 DAJVQG010000000
VERSION     DAJVQG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26013
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.044"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            42..1238
                     /gene="hemY"
                     /locus_tag="OQM83_001657"
     CDS             42..1238
                     /gene="hemY"
                     /locus_tag="OQM83_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCS1519943.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            1900..2061
                     /locus_tag="OQM83_001658"
     CDS             1900..2061
                     /locus_tag="OQM83_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1519944.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(2145..3380)
                     /locus_tag="OQM83_001659"
     CDS             complement(2145..3380)
                     /locus_tag="OQM83_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCS1519945.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(3527..3603)
                     /locus_tag="OQM83_001660"
     tRNA            complement(3527..3603)
                     /locus_tag="OQM83_001660"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3567..3569),aa:Pro,seq:tgg)
     gene            complement(3646..3732)
                     /locus_tag="OQM83_001661"
     tRNA            complement(3646..3732)
                     /locus_tag="OQM83_001661"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3696..3698),aa:Leu,seq:cag)
     gene            complement(3753..3828)
                     /locus_tag="OQM83_001662"
     tRNA            complement(3753..3828)
                     /locus_tag="OQM83_001662"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3793..3795),aa:His,seq:gtg)
     gene            complement(3887..3963)
                     /locus_tag="OQM83_001663"
     tRNA            complement(3887..3963)
                     /locus_tag="OQM83_001663"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3927..3929),aa:Arg,seq:ccg)
     gene            complement(4064..5449)
                     /locus_tag="OQM83_001664"
     CDS             complement(4064..5449)
                     /locus_tag="OQM83_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1519946.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(5640..6380)
                     /gene="rffM"
                     /locus_tag="OQM83_001665"
     CDS             complement(5640..6380)
                     /gene="rffM"
                     /locus_tag="OQM83_001665"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCS1519947.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6383..7735)
                     /gene="wzyE"
                     /locus_tag="OQM83_001666"
     CDS             complement(6383..7735)
                     /gene="wzyE"
                     /locus_tag="OQM83_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCS1519948.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7732..8811)
                     /gene="rffT"
                     /locus_tag="OQM83_001667"
                     /gene_synonym="wecF"
     CDS             complement(7732..8811)
                     /gene="rffT"
                     /locus_tag="OQM83_001667"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCS1519949.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8808..10058)
                     /gene="wzxE"
                     /locus_tag="OQM83_001668"
     CDS             complement(8808..10058)
                     /gene="wzxE"
                     /locus_tag="OQM83_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCS1519950.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(10060..11190)
                     /gene="rffA"
                     /locus_tag="OQM83_001669"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(10060..11190)
                     /gene="rffA"
                     /locus_tag="OQM83_001669"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCS1519951.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(11195..11869)
                     /gene="rffC"
                     /locus_tag="OQM83_001670"
                     /gene_synonym="wecD"
     CDS             complement(11195..11869)
                     /gene="rffC"
                     /locus_tag="OQM83_001670"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCS1519952.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAQLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11847..12728)
                     /gene="rfbA"
                     /locus_tag="OQM83_001671"
     CDS             complement(11847..12728)
                     /gene="rfbA"
                     /locus_tag="OQM83_001671"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCS1519953.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12747..13814)
                     /gene="rffG"
                     /locus_tag="OQM83_001672"
     CDS             complement(12747..13814)
                     /gene="rffG"
                     /locus_tag="OQM83_001672"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCS1519954.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(13814..15076)
                     /gene="wecC"
                     /locus_tag="OQM83_001673"
     CDS             complement(13814..15076)
                     /gene="wecC"
                     /locus_tag="OQM83_001673"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCS1519955.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(15073..16203)
                     /gene="wecB"
                     /locus_tag="OQM83_001674"
     CDS             complement(15073..16203)
                     /gene="wecB"
                     /locus_tag="OQM83_001674"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCS1519956.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(16259..17305)
                     /gene="wzzE"
                     /locus_tag="OQM83_001675"
     CDS             complement(16259..17305)
                     /gene="wzzE"
                     /locus_tag="OQM83_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCS1519957.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(17317..18420)
                     /gene="wecA"
                     /locus_tag="OQM83_001676"
     CDS             complement(17317..18420)
                     /gene="wecA"
                     /locus_tag="OQM83_001676"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCS1519958.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18660..19919)
                     /gene="rho"
                     /locus_tag="OQM83_001677"
     CDS             complement(18660..19919)
                     /gene="rho"
                     /locus_tag="OQM83_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCS1519959.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(20004..20105)
                     /gene="rhoL"
                     /locus_tag="OQM83_001678"
     CDS             complement(20004..20105)
                     /gene="rhoL"
                     /locus_tag="OQM83_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCS1519960.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(20246..20575)
                     /gene="trxA"
                     /locus_tag="OQM83_001679"
     CDS             complement(20246..20575)
                     /gene="trxA"
                     /locus_tag="OQM83_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCS1519961.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20706..21971
                     /gene="rhlB"
                     /locus_tag="OQM83_001680"
     CDS             20706..21971
                     /gene="rhlB"
                     /locus_tag="OQM83_001680"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCS1519962.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            22105..23589
                     /gene="gppA"
                     /locus_tag="OQM83_001681"
     CDS             22105..23589
                     /gene="gppA"
                     /locus_tag="OQM83_001681"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCS1519963.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(23636..25657)
                     /gene="rep"
                     /locus_tag="OQM83_001682"
     CDS             complement(23636..25657)
                     /gene="rep"
                     /locus_tag="OQM83_001682"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCS1519964.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         6576 a         7079 c         6452 g         5906 t
ORIGIN      
        1 caaggtgata ctccggcagc cgcgccgcaa ggagaataac catgctaaaa gtgttattac
       61 tctttgtttt gctgattgcg gggatagtgg ttggcccgat gattgccggt catcagggtt
      121 acgtgctgat ccagactgac aactacaata tcgaaaccag cgtcacgggc ctggcgatta
      181 ttttgattct ggcgatggta gtgctgtttg ccattgagtg gctactgcgg cggatcttcc
      241 gcacaggcgc acacacccgt gggtggtttg tcggacgtaa gcgtcgccgt gcccgtaagc
      301 agaccgaaca ggcgctgctg aaactggcgg aaggcgatta tcagcaagtt gaaaagctga
      361 tggcgaaaaa tgccgatcac gcggaacaac cggtggtgaa ctatctactg gctgccgaag
      421 ccgcgcaaca acgtggtgat gaagcacgcg ccaaccaaca tctggaacgc gcagcagagc
      481 tggccggcaa tgacaccatt ccggtagaaa tcacccgtgt acgtctgcaa ctggcccgta
      541 atgaaaacca tgctgcacgc cacggcgtgg ataagctgct ggaagttacg ccacgccatc
      601 cggaagtatt acgtctggcg gaacaggcgt atatccgcac aggtgcatgg agttctctgc
      661 tggatatcat cccatcaatg gcgaaagccc atgttggtga tgaagaacat cgtgcaatgc
      721 tggaacaaca ggcatggatt ggcctgatgg atcaggcgcg tgccgataac ggtagtgaag
      781 gtttgcgtaa ctggtggaaa aaccaaagcc ggaaaacgcg tcatcaggta gcgttgcagg
      841 tggcaatggc ggaacatctt attgaatgtg acgatcatga tactgcccag caaattatca
      901 tcgatggcct gaaacgccag tacgacgatc gcctactgct gccgattcct cgactgaaaa
      961 ccaacaatcc ggaacagctg gaaaaagtgc tgcgccagca gatcaaaaac gtcggcgatc
     1021 gcccgctgtt gtggagcaca ctgggccagt cactgatgaa gcacggagaa tggcaggaag
     1081 catcgctcgc cttccgcgca gcgctgaaac aacgtccgga cgcctacgat tacgcatggc
     1141 ttgccgacgc gctggacaga ctgcacaagc cggaagaagc tgcagcgatg cgtcgcgacg
     1201 gtttgatgtt aacgttgcag aacaacccac cacagtagtt ccttctcacc cggaggcaag
     1261 cacctccggg acctttctga tgcataaaaa aacgcctgct cttattacgg agcaggcgtt
     1321 aaaacaggtc tgtatgacaa cagtgggtgc ttcactcaac gttgtgtcca tggtgtctga
     1381 tgaggcataa gcgacatctg tcagtggacg ataagcaccg taaacggctc tgcgtcattc
     1441 cggagtttat gaggcactaa ggcgaacata agagatggaa tgagcatcta cccgtttatt
     1501 atgccacaga gaatcgggaa ataacatccc ttaacacttg ttatgagata attctgtaat
     1561 cctctttgct tcctgagtga atatttaacc tgagcttgat cctacacata cttattatga
     1621 atgataaaat tcattcaatt aataacacat atactaattg ccgttaaaac taaaaaagca
     1681 ttaataatca acgcaatata ataaacccgc cttacatatc aactgcgcca gaggtaggat
     1741 tgaaaacgct ctcctgattt tccaattcat tttctggata aataaataat ttatttttgt
     1801 cactattatt tatgtaatca tcctgtcagg gagagggatc tcaattatca atgcttaatt
     1861 acgtcatcat tttgatgaca tgaaaccgga gcattttgca tggaattttc gttttcaccc
     1921 aaacttcttg ttgttgctgt cgccgccgct cttcctctca tggccagcgc agcagatacc
     1981 ccgtcaactg ccaccgcacg caaaggcttt gccggatacg atcacccaaa ccagtatcca
     2041 cagaacgctg caatttattg aatttgcacg atcatgtagg ccggataagg cgtttacgcc
     2101 gcatccggca atcaaccgca ggcggccaca aaatggccgc ttttttactt attcaccacc
     2161 agcaaatgtg cctgcataat gtcactggcc gggcgaccgt gcgccagcaa atcagccatt
     2221 gctttaagat atggcggtag atggcggaaa taacgctgat acccggcaca caaataattc
     2281 agccccggtt tgccgctggc atcgagcatg aagcggtgtt tcgggcagcc tccccagcac
     2341 gcttttaaca cgttacaact gcgacactgc gccggtaatt gcttaaattt atcttcacca
     2401 aacacctgct gttgcgggga atcgatcatt tctgcaattg tttgctgatg catattcccc
     2461 agccgatatt gcggataaac atagtgatcg caggcgtaaa cgtcgccgtt gtgctcaaca
     2521 atcaccgagc gcccacaggt tggctgatga tggcaaaccg cacccggcgc accgacaaaa
     2581 ttggcaaacg cccattcgat attcatcacg aaaatcttgc cgacgtcgcg tttgatccag
     2641 tggtcgaata tcgccaccag aaattcacca aattcatcgg ggcgcaccga ccattccgtc
     2701 agttccccct gaatatcacc aggcgcatgc aatttcagtc catcgcgggc agctgtttca
     2761 tcagccagac gctcgaccac cggaataaac tggatgaatt cgactcccgc atcgcacaaa
     2821 aaatcataaa cctgcaacgg ttgctgcgcg ctggtgcggt tgacgcagac cagcacgtta
     2881 tagtcgacat gatgtttttg cagcagcgtc agggcacgca tcaccagctt atgcgttggt
     2941 ctgccacctt tggtcacgcg atattgattg tggatctcag ccggaccgtc cagcgataac
     3001 ccaacaagaa aatgattttc tgccagaaat gcacaccatt tatcatcgag cagcacgccg
     3061 ttagtctgga agctgttact tatcttcctg ccagcaccgt atttcgcctg taactttacg
     3121 gcacggcggt aaaaatccag cccgagtaac gttggttcgc cgccctgcca ggtaaaagcc
     3181 acttcgtttt gcggttcgct ggcagcgata tagtgacgga catacgcttc cagcgtgtca
     3241 tcgtccatat gcgtgactgg cttttcgcgg taaagggatt gtttttcgag ataaaaacag
     3301 tagtcacagt tcagattaca atcggaacca ctcggtttcg ccatcacatg aaaagcacgc
     3361 gttggaacct gttgcagcat ggtcactcct tgcctgaagt catccttcaa tacgttaagg
     3421 gcgaaatcat accgtggggc aatgagttat tacgggcgtt tattgcgaag ggagggtgac
     3481 gaaatgcaca gaaaacaaaa aaccccgccg aagcggggtt caaaattggt cggcgagaga
     3541 ggattcgaac ctccgaccca ctggtcccaa accagttgcg ctaccaagct gcgctactcg
     3601 ccgaaatact gctttttgaa tttttagttc aattctttaa agtcgtggtg cgaggggggg
     3661 gacttgaacc cccacgtccg taaggacact aacacctgaa gctagcgcgt ctaccaattc
     3721 cgccaccttc gcattgtcac aacttctaat aatggggtgg ctaatgggat tcgaacccac
     3781 gacaactgga atcacaatcc agggctctac caactgagct atagccacca ctacaaatct
     3841 tgttacgcgg tattactacc accgcagctc aagcgccggg actaaatggc gcgcccgaca
     3901 ggattcgaac ctgagacctc tgcctccgga gggcagcgct ctatccagct gagctacggg
     3961 cgcttagcgc cgttgcgggg ggataatacg gacttcccac ccctctgtct agtccctttt
     4021 taaataaatt gcgcgtttgg ttacggtttg tgcgttttgc tgcttattcc tccagtttat
     4081 gcgctttccc gtggcgatta aggccaaaaa ctttataaat cgccgtcacc gccagcataa
     4141 agatgatgcc aacaaacagc gacatacgcg tatcttcatt aaagtacatg ccgatcaaaa
     4201 cgcaaatcag gaatgccatt gttacgtagt tggcccacgg gaacaggatt gagcggaacg
     4261 gatggctggc aatcgccgct ttatgtgcac gccgaaaacg cagctggctt atcaatatca
     4321 caaaccatgg caccatcccc ggaagcacgc tggcactgta gacgtagaca aacacacgct
     4381 gcggattggg aatgatgtag ttcaggcatg agccaattag cagaatagca atagatactg
     4441 ccacacccgc aactggtacg ccgtgacggg aaactttcgc catcgccgcc ggtaactgac
     4501 ggtttttcgc cagtgcgtag agcatacgtc cgcaactgta catgccgctg ttacagccag
     4561 agagcgcagc cgtcagcacc acaaagttga taatgcccgc cgctgcggta ataccgattt
     4621 tggcaaaagt cagtacgaac gggctgccgt tgctgccaat ttcattccac gggaagatgg
     4681 tgacgataac gaaaatcgcg cctacgtaga aaatcaggat ccgccacagc actttgccta
     4741 cagcactgcg cagcgtcacc tgcggattct tcgcttcacc ggcagtaatg ccaatcagct
     4801 ccacgccctg gtaggacgcc accacaatac acagcgcggt caggaaccct ttccagccac
     4861 ccgcaaagaa accgccatgc tctgtgagat tgctaaaacc aatcgactgc ccgccattgc
     4921 caaagccaaa gaaaatcacg cccaggccaa tgacaatcat cacgataatc gtggtgactt
     4981 tgatcatcgc gaaccagaac tcgatttcgc cgtacaaccg caccgccgcc agattcgcca
     5041 acgccaccag cgccaccgcg atcaatgcag gtatccactg cgccatctcc gggaaccaga
     5101 actggacata aacgccaatg gcggtgattt ctgagatccc caccgccatc cacataaacc
     5161 agtaagacca ggcggtgaga taaccaaaaa acgggctcat ataacgatgc gcataaacgg
     5221 cgaacgaacc ggtaacaggt tcaaggaaca acatttcgcc cattgaacgc atgatgaaaa
     5281 agacgaacag cccggcgatg atataggcca acaatacgga tggcccggcc catttcaggg
     5341 tactggcggc ccccataaac aggccgacgc caatagtgcc ccccagggcg atgagttcga
     5401 tatgtcgagc ttccagccca cgctgtagct ctggtttgtt atctgccata aatcctcgtg
     5461 ttgtgtttgc atgctttccg gtgttaccgg ttatcgttat gggtacatcg agtgttgcga
     5521 atgttttcgt aattcaggag aaatggcaaa taaagcatta aaaatttgaa tgctttgtgt
     5581 aataaaaaag cagacaggcg acggagtgac cactccgtcg ctttacaaag agaggaaaat
     5641 cagaggttgc cggtgtagtg ccagcgtaaa taacgcagca aacgaagctg acgcttaatg
     5701 cggctcggct gcgaaagcag gcggtagagc cactccagtc ccagcgtttg ccagattttc
     5761 ggtgcgcgtt ttacgtgacc ggtgaaaaca tcgtaagtcc cgccaacgcc catatacagc
     5821 gcatctggat gcaccagacg gcagtcgcgc atgaagatct cctgctttgg cgatcccatc
     5881 gcaacggtga cgatttgcgc tccgctggca tgaatgcgtt caaacagcgc ctgacgctgc
     5941 tcgggtttaa aataaccatc ctgactgcca acgatattca cattccattg gttgcgcagt
     6001 ttagcttcag tttgcgccag cacttcaggt ttaccgccca caagaaatac cggcgtccct
     6061 tctttgcctg cgcgcgccat cagctcttcc cagagatcgg caccggcaac gcgggaaacc
     6121 tgcgcctgcg ggtacttttt acgtactgaa cgtacaacgc tgatgccatc cgcatattta
     6181 aattcggcag cgttaattaa ctccctgacc tcggcgttat cttcaatggt cagcattttt
     6241 tcagcattaa tggcaaccag cgttccctgc ttaagctgcc cgtcagcaaa cagataatcg
     6301 agggcgtgct gcatatcacg ccaaccaatc aactgtaagc cacgcagcgt ataggtcggt
     6361 gccgtggtgt tgttattcat tgttatcctt caacctgcgt ccggagcgat gattttgtac
     6421 gtttatgaat gagtccggcg ctttcaaaaa gccagtacag cagttttgcg atcatcagac
     6481 atgcgccgaa gaccacgata aaaaagacta cgcgtgagac aaacgaatcc agcccttcac
     6541 gcgccagcac gatcatattg aagatcgccc caaagcagaa actgtgcaat atcgcagcct
     6601 tatagcgatt aggctcgcgg ttgcccagct catacagcca gtcgaaccat ttgatgatca
     6661 gaccaaccac gatcgccccg agcgggatga acaacgcgcc gcccatcacc accagtgagc
     6721 ctataagcgt aggcgagatc gccagtccgg agtggttatt cagcacttcc caggtaaagt
     6781 agttggctga gttcagcacc atactcgggc gacccggcca cagccaggaa gggataaaga
     6841 catagaaatc gcggacaatt ggagccaggc cctggaagtc gatgttgtcg tagttctgca
     6901 acagcaacgc cagattctcc cacggggaga aggtgtcgcg agtgagatag agaaacgtat
     6961 agaacgcttc atcgccgctc acattcattc catagcgttt tagcgccagc cagaacatgc
     7021 caacaatccc cagcacgccc gccgccgcca gcatccacaa cgaaatccag ccgcgaataa
     7081 tgccaataaa caggaagata gcgaatgcga tgatgatatt ggcgcgagtg ccgccgacaa
     7141 tcatataagt cagcaagcca aaggcgaccg tgctgacgag gaaaaacagc cacgctttgc
     7201 tgtcctggcg cagaaagtag accaccagca tcgccgggat gaaaaagtaa aagaagcgtt
     7261 ttaacgccac gccggagact tcactggaga agatctgact gtaggagtta agccggaaca
     7321 gcaaaaagcc gttgtgcatg aagaagatgc cgacgcttac cagcgcgata cccatcagga
     7381 tcacccacgt aagattggtc tccacgcggt tcatggtaaa cagcggacgg cgcggtacat
     7441 cagcaacgcg tttgcgtagg cgggttttgt aggtgacata gtaaaccgcg tagaagcagc
     7501 ccgcagaaag caacgcctgc aacaagattt ctggcggcgc gacaccaaca tcaaagcgaa
     7561 ataccagcac gctggtcagc gggaagccga agaaaaaggt gagcaaaaac agcaatgaaa
     7621 agaagacatt gaagttaaag cgcacacggc gaaactcaaa ccaggtcagc gtggcaataa
     7681 acagcgtgca gagcagccag accacaaaca ggccactgaa ttgcagcaga ctcatgcgac
     7741 ctccccggcg gcaatagcca acgcccgctg ccagccttgt agatagttag ggctaaagaa
     7801 ggcaatggtg tttttatcca ccgacgccaa ctggcgctgc gcttcacgca caatatcctc
     7861 gttgagatcg tcggtagtaa acagcaccgg caaatgctgt tccgtcatat cctgccagaa
     7921 cggattttcc cggttaagca cacaaggaat gcccgcctga atcagtaagc aaagcgtacc
     7981 aatgccctgc tggcgggcaa aaataaagta accaagatcg cactgacgaa gtagcgccag
     8041 ataggcgtca aattccagtt tttcgctcaa aatttgcaga ttttcttcgc tgaataactc
     8101 cagccccgcc tgacgaactt cctcaatgta cgcttcgtta ttaggcggat atcccatcgg
     8161 caccaccact tttaccgtat cgccaaattg ctgatgaacg gcgcgcaagg cagcaatatg
     8221 gtcattgctg cggtcaccgg agttccccac cagaatggtc attttccctt cacgttgccg
     8281 atcattcgcc atcgtattga gagaagggtc catccgcgtc gggaagtaca gcagttcgcc
     8341 ccgcaccttt ggatgcgttt tggcaaaaaa gctcaaatca ccgcgggtgg caaatacaca
     8401 gccgactcgc ttttgcgcca ggcgacgtag cgggtaaaaa agcttatatc tcaagccact
     8461 ggaaagctca tacaggtctg ccccccagat atgccagaaa aactggctgg gcttaatgcc
     8521 accactcagc agagccagcc acagtgtggg attgaactga ccgtggaaaa aaaatcgctg
     8581 ctgacggtta gcttttgctt tcgcgatgac cgcttccgcc agcgattttt tcccagggaa
     8641 aaattgcaca gaaagcgccg gacagctatc acttaagccg tcgtccttgc caacaaccat
     8701 aaactcgcgc gcgtgctcgc tcgtcgcggc cagcgcgtca ttgaaaaacc gcaaaacggt
     8761 tcggttatgg tgagggatat ccgatcccag tacgtgaatc agtacagtca tgcccgccta
     8821 cgccagagta aaaacacgcc acaacaaaga gaaaaataga cgatataagt tgccatatat
     8881 gcctgcgccg cgcccagtgc accatgcgca gggattagcc agtgggcaaa taccatcaat
     8941 aaagtgaact ggctgacttc cgccagaata taaaaccgca gtgacgcttt ggcgatcacc
     9001 agataaccaa agacataagc gcccactttt aacacatcac ccactaactg ccaggcaaag
     9061 agatcgcgca tagcggtaaa tttattcgac aacagcaacc agatagcaaa atcacgcagc
     9121 aaccagacgg taaaactcgc cgccgccact gccggtaaga cgaatttcag cgatttaacc
     9181 acttcccggg tgatatcgcg cttttccgtt agccgcgaca acgtgggcag caaatatacg
     9241 ctgaacgatg ccgtaataaa ttgcaggtag gcatcggaaa tactgctcac cccttgccag
     9301 atccccactt catcccagct atactgcgcc gccagcagtt tacgcatcat gatgtaagca
     9361 acaggcaagg tcaccgacgt aatcaacgcc atgagcgtaa atttgctcaa ctgccctgcc
     9421 agaccgttat cccagctggg tttcagatag cttaacggga tgacaccacg tttgatcaac
     9481 atgatggcgg caggaatcac caccagcgcg ggaatcagcg ccagacccag caacgcccct
     9541 tcataaccgc ccaaacggta actgacgtag tacgcgagaa cgccaatcaa gctgccgaca
     9601 atcagcgata acgcattacc tgcggcatcg cgaaagcctt tcatcagcgc cagtaacagg
     9661 ttgccccagg cgatccccat ttgcaccagc gccactaaac gcaccagccc ctgatagtcg
     9721 gtattaccaa acagtccctg gctgattggc gcagctgcca gcacaaaaac cagcgccatc
     9781 agcgtagaga aaccaagtac catcgctgat gaagtgccga ccacgcggcg cagctgttgc
     9841 ggattatcat ggtactgggc aacgtatttg gttacaccgt taaagatgcc agctccggca
     9901 agcacgccga gcacggtaat caactggcgg aaatttgccg ccagcccaag ccccgccgga
     9961 ccaaatgaca cagccagcaa cttaccgacc agtaacccgg caccaatctt gaccagtgta
    10021 ctggccgccg tccacaagga cgcttttgcc aacgacatat caggagaagt agttcaacaa
    10081 agtcgcaatt accgtacgct gattgacggg cgacaggttg tagaacagcg gcaggcgcag
    10141 caggcgctcg ctctctttgg tggtgtagcg atcttcaccg tggaactcac caaagcgttc
    10201 ccccgcaggg caaccgtgca gcggaatgta atggaacacc gccatgattt ccgcttcttt
    10261 cagaaagtta atcaacgcgc tccggtcatc aatatcccgc agtttaatgt agaacatatg
    10321 cgcgttctgc acgcagccat cgggaatcga cggcagctcg atacgcccgg ctttcgccag
    10381 aggcgctaac gcatcgtagt agttttgcca cagcgccaga cgttgctggt tgatacgatc
    10441 cgctgcttcc agttgcgccc acaggtacgc agcttgcaga tcggacatca aatagctgga
    10501 gccaatatcg cgccaggtat atttatcgac ctgaccacgg aagaactggc tgcggttagt
    10561 gcccttttca cggatgatct cggctcgttc gattaacgct ttatcgttaa tcagcgtcgc
    10621 gccgccttca ccacccgccg tatagttttt ggtttcatgg aagctaaagc agccaatatg
    10681 accaatggtt cccagtgcac gccctttgta agtggacatc acgccctgag cggcatcttc
    10741 taccacaaac agattatgct ttttcgccaa cgccataatg gtgtccattt cgcaggccac
    10801 acccgcgtaa tggaccggca cgataacgcg cgttttgtcg gtgatcgccg cttcaatcag
    10861 cgtttcgtcg atgttcatgg tgtccgggcg aacatccaca aaaacgattt ttgcgccacg
    10921 cagcacaaag gcattggcgg tggagacaaa ggtgtagctc ggcatgatca cttcatcgcc
    10981 aggctggata tcgagcagca gcgccgccat ctccagcgaa gcggtgcagg acggcgtcag
    11041 taacactttg gcgctgccaa aacgttgctc cagccactgc tggcagcgac gggtaaaacc
    11101 gccatcgcca cacagtttgc cgctacccat tgccgactgc atatagtcga gttcggttcc
    11161 caccaccggc ggtgcgttaa atggaatcat gtgatcacct gtataaccag tacgcggtgc
    11221 tttctacatt cgcaccactt tgtatgtatc gtttaagcgc ggcggtgttg cccatttggg
    11281 tcgccacccg caaagttgtt ttaccgcgag catacgccca gtttagcgcc gtttgcatca
    11341 gctcagcacc tgcaccgcgt ccagccagca ggccaattcg cgcatctgtc gcattgagtt
    11401 cccgtaaaga gacataaccg cgaataccgc cggacgccgc acgtaaaatc agacattgat
    11461 gatcaaaggt gccgcgcacg gcattttcaa tccactgtgc ataaaagcgt ccgctggcgt
    11521 caggcgcata ccacggcgca cgaaaacggc tttgcgcaaa tgcggcgctg gctaactgac
    11581 gtaatgcggg aatatcggtc tcttgtgcca ctacagcacc actatcactg acattgttca
    11641 cgggtagtgc caaatcaact tcaccttcta ccagggagaa tcccagctgt tgcagggcat
    11701 ccagttcacc cgtatttgat gccgcaattt tggcctgcac ccgtgaccac ggcgctaaca
    11761 cgtctggcgt caggagctgt gcttcagacg taatgcgcac gatggcgctg ttaacaccaa
    11821 agaaggcgtt ttcccaggtt agtggctcaa tactggcgcg gacgggcacg aagtaactcc
    11881 agcagatatt ggccgtagcc agttttcgct aatgaactgg cagcacgctt cacaccctcg
    11941 tcatcgagcc agccgttacg ccaggcgatc tcttccaggc aggcaatctt aaagccctgg
    12001 cgtttttcta ctgtctgcac aaaggtactg gcttcaatca ggctgtcgtg agtgccagtg
    12061 tccagccagg caaatccgcg cccgagcagt tcaacggtca ggttgcccgc ctcgaggtac
    12121 atctggttga tggaggtaat ctccagttca ccacgctccg acggcttcac ctgctttgcg
    12181 tactccacga ctttactgtc gtagaaataa agcccggtca ccgcccagtt tgacttcggc
    12241 tgttttggct tttcttccag cgagatagcg cggaaattgt cgtcaaactc caccacgcca
    12301 aagcgttccg ggtccatcac ctgatagcca aataccgtcg ccccttccgt gcgcgccgca
    12361 acatgacgca gcttcggact gaagccctga ccgaagaaga tgttatcgcc cagcaccaga
    12421 caagaaggtt caccgttgag gaaggtttca ccgatgataa aggcctgcgc cagaccgtcc
    12481 gggctgggct gttcggcgta ttccagctgg ataccgaact cactaccatc gcccagcagg
    12541 cgctggaaat aacctttatc ttccggcgta gtgatgatga gaatttcgcg gatatcggcc
    12601 aacatcagca ccgacagcgg atagtaaatc attggcttat cgtaaatcgg caacagttgc
    12661 ttcgatacgc cgcgcgtaat cggatgcaat cgggtgccgg aaccgcccgc caggataata
    12721 cctttcatgt ttgcctccgc tgaaaattag cctttcagac ctaaacgctc gccctgatag
    12781 ctgccgtcct gcacctgctt ccaccagctt tcattagcca gataccactg caccgtttta
    12841 cgcattccac tttcaaaggt ttcctgtggc agccagccaa gttcacgggc aattttcgaa
    12901 gcatcaatgg cataacgcag atcatgcccc ggacggtcag cgacaaaggt gatcaagtca
    12961 cgatagtgca ccacgccgtg cggcttgttc ggagccagtt cttccagcag ctcgcaaatg
    13021 gtttccacaa catcgagatt cttacgctcg ttgtgaccac caatattata ggtttcaccg
    13081 actttcccgg tggtcgccac gcaatacagc gcgcgggcgt gatcttccac atacagccag
    13141 tcacggattt gctgcccgtt gccatatacc ggcagcggtt tacccgccag cgcgttgagg
    13201 atcatcagcg ggatcagttt ttccggaaag tggtaaggac cgtagttatt cgagcagttg
    13261 gtgataagcg tcggcagacc gtaggtccgc agccaggcgc gcaccagatg gtcgctgctg
    13321 gcttttgacg cggaataagg gctgctcggc gcatacggcg tggtttcggt gaagaaatca
    13381 tccgtcgagt gcaggtcacc atatacttcg tcggtggaga tatgatgaaa acggaacgct
    13441 gattttttat cttccgtcag cgtattccag taagcccgcg ccgcttcaag caatgtataa
    13501 gtcccgacaa tgttggtttc aataaacgct gccgggccgt caatagaacg gtcaacatgg
    13561 ctttcggctg ccagatgcat gacacagtct ggctgatgct cagtgaatac gcgtgccagt
    13621 tctgcccgat cgcagatatc aactttctca aaggcaaagc gctcgctttg cgcgaccggt
    13681 gccagcgaca tcaggtttcc ggcgtaggtc agcttatcga ccactaccac cgcgtcgctc
    13741 gtttcgttga tgatataacg caccagcgcc gagccaataa acccggcacc acctgttatc
    13801 agaatttttc tcatcagcgc cagactcctt tggcatcgac gacatactgc tgatggacat
    13861 tgtcgccatt gataacttta aactgactat gatcgaccag catcaccagc acgtctgccg
    13921 ttgccagcgc ctcgtcaagc tgcgccagag tacaaagccc ggtcagtttt ttcggcaact
    13981 ggtggatgtt aggctcaaca accagagttt cgccgctatg ccactgggcg atcagttcag
    14041 cgatttccat cgccgggctt tcgcgcaggt catcaatatt cggtttaaac gccagaccaa
    14101 agcaggcgat tttcagttca ctggcgcgtt tatcggtagc cgccaggcaa tcagccaccg
    14161 ccgctttcac ctgatcgata acccagaacg gtttgtgatc gttcacttcg cgcgcggtac
    14221 ggataagccg cgcctgctgg gggttctgtg ccacgataaa ccacggatca acagcaatgc
    14281 agtgaccgcc cacgccaggg ccaggctgaa gaatattaac gcgagggtga cgattcgcca
    14341 ggcgaatcag ttcccagacg ttaatcccct gatcggcaca aatcagcgac aattcattag
    14401 caaaagcgat attcacatcg cggaagctgt tttcggtgag cttacacatt tccgccgtcc
    14461 gcgagttagt gacgacacac tcaccttcga ggaaaatttt gtacagttcg ctggcgcggg
    14521 tcgaacaaac cggcgtcata ccaccaatca cgcgatcgtt tttaatcagc tcgaccataa
    14581 cctggcccgg taacacgcgt tccgggcagt aagcaatgtt gacgtccgcc tgctcgccca
    14641 cctgctgtgg gaaagtgaga tccggacgca tctctgctaa ccattctgcc atcttctcgg
    14701 ttgaccccac cggcgaggtg gattcaagga tcaccagcgc gccttttttc agcactggcg
    14761 caatggagcg agcagccgat tcaacgtagg tcatatctgg ctcatgatcg cctttaaacg
    14821 gcgtgggtac agcaatcagc caggcatccg cttcaactgg cgtcgtgctc gctcgtaaaa
    14881 aaccgccttc tacggcagtt tttactacgc tcgccaaatc aggctcgacg atatggattt
    14941 cgccacgatt gatggtatca accgcgtgtt ggttgatatc gacaccaatc acctgttttt
    15001 gccgtgaggc aaacgctgct gctgtaggca gcccgatgta acccagtccg ataacagaaa
    15061 tggtcgcaaa actcatagtg atatccgatt attttttaac gcttccagaa tgcgagagca
    15121 tgcctgacca tcaccatacg ggttatgggc gcggctcata gtttgatatt cgttttcgtc
    15181 ttttaaaaga cgcgtcactt cctcgacaat tcgctgctta tccgtgccta ccagacgcac
    15241 cgtacccgcc gtcaccgctt ccggacgctc agtggtatcg cgcatcacca gcaccggttt
    15301 ccccagcgaa ggcgcttctt cctgaatgcc gcctgagtcg gtcaaaatca gccaggcgtg
    15361 gttcatcagc cagacaaacg gtaaatactc ctggggatcg atcagaatga catttttcac
    15421 atgccccaga atgcgattga ccggctctct gacgttcggg ttgagatgca ccggatagac
    15481 aatctggatg tcctggtgcg tggtggcaat gtctgccaac gcgtggcaga tttcttcaaa
    15541 gccacgaccg aaactttcac gcctgtgacc ggtcaccaga atcatctttt tatcggggtc
    15601 gataaacggg taatttgccg ccagttctga acgcagcgta tcgctgctca tcacctggtc
    15661 acgcacccat aacagtgcat caataactgt attaccagta atgaagattc ggctatccgc
    15721 aacgttttca cgcagcaagt tttgccggga agtttcggtt ggagagaagt gatacatcgc
    15781 cagatgcccg gtcaatgtac ggttagcctc ttccggccac ggcgaataaa gatcgcccgt
    15841 gcgcagacca gcctcaacgt gaccaacagg aatacgctga taaaacgccg ccaggctggt
    15901 tgccagcgtc gtcgttgtgt cgccgtgaac cagcacgacg tctggtttga actcggcaag
    15961 aataggtttt agcccttcca gaatccgaca ggttatctct gtcaggccct gtcctggctg
    16021 cattatgttg agatcgtagt caggtacaat ggaaaagagt ttcagcacct gatcgagcat
    16081 ctcccgatgc tgcgcagtga cgcaaacttt agcctcaaaa aaaggatctt ttgccaacgc
    16141 atgcaccaac ggcgccatct tgatggcttc cgggcgcgta ccaaatacag tcagtacttt
    16201 cacatcgatt ctcttcgaat aagcggcgag cgcctttgcg ctcaccgcag cagtgttgct
    16261 atttcgagca acggcgggtt aatgcgacac cagccccgat cagccccccg acaatgcccc
    16321 acataatcat caggaaggca cgacgtgggc tatcgcgttt taccggttct tccggcgtac
    16381 gcaaatagcg ataggtctga aaacgcggat ccagggttgg accaacattc agggtgttta
    16441 acatggcccg attctgatca tagtcgagat caaaggccgg accgacggcc tgtaaatttt
    16501 ccagtcgagc ctggagcatt ggacgcccaa gcaggaacat ttctgaatca ggtaattcct
    16561 cggcaggcac atctgtcgca ctgcgcgaaa tattatgctg ctcagcaatt ttcagcgcct
    16621 gttcaatact gttcatccgg cggtcgtaga tggctttcgc cacctcttcc tgacgcttca
    16681 cctgtgcttt catctggatg gtacgcgccg cccatgcgcc tttcagctca tcattcagat
    16741 ggctggctgc acgctggctg gcaaaagcaa catactgacg taacaggtta ttagcgtcag
    16801 gcgcggtttc agcaataagc ttcacgctgt cattgaccgc gcgggtaaag tctccgggga
    16861 taaactggat gttgttaatc atttcatcca gcaacgccgc atcggctttg ctgttgccca
    16921 ccatccgctg tttgtaatag tcggtttgca gccagaactc tctgcgggta tcccacgagg
    16981 ccagttgcat aacaaactct ttataggctt cgtccatgac cgatggttgg tcggcagaag
    17041 ccatgtttga acggacatcc aggttacgca aaaattgctg ctgcgagtaa tatcccccca
    17101 gcatattcac cgttggacga tcggtaatcg ccgtcgagct ccactcctga cgagcaaaaa
    17161 aagtatacgc cagcgcgatt aacgcaaatg ccagccccat gccaataatc catagcttcc
    17221 cagcccacaa ggtacgaaac aacccacgaa tatccagttc attttcagcg tcttcggccg
    17281 gtttcccagg cattggttgt gtcatcacat cctcatttat ttggttaaat tggggctgcc
    17341 accacgattt ctacgcagtc tgcgttttac gcgcttaata aagcgagcaa ctttccaggc
    17401 acgcttaatg caatatccat agaggaagaa tgctagcaaa aagagcacca gcatgaccca
    17461 ctccgggaca aaatgagaat attctgccag cacgccaatg gaagcgagca gtgccgcagc
    17521 aagggtaatc agcacaaacg cctgacggga agtaaacccg gcacgcatga tcaaatggtg
    17581 aatatgctga cggtcaggag agaatgggct catgccttta cgcaggcgac ggtacataat
    17641 cgccaccata tccattagcg gaatggcgat tatccacaaa gcggtaaccg ggctgatggg
    17701 atgggttttg ccctgggtcg tttcgagcag gatccagata acggtaaaac caatcagcgt
    17761 actgcccgca tcacccataa agactttgta gcggcgaccc aggataccaa ggttaagcat
    17821 gatgtatggc aggatggcgg cgatcatcgc aaagcaccag attgcgaggc tggtttgccc
    17881 gtcgaaccac aaaatcatac cgattgctgc aaacgagacg caggacaacc cgcccagcaa
    17941 gccatcaatg ccatcaacca tgttgaacgc attaatggcc gcccagacgg caaatagcgt
    18001 caggaagtaa ccaaacggtc cgagcaccat ctcccaggag ccaaagatat aacccaggct
    18061 actgagataa agtttgccga acaccatcat aacaatgcca acagcggcct gtatggtggc
    18121 acggattttt acgctgatat caaaacggtc atccagcgcg ccaatgaaaa caagcacacc
    18181 ggcacaagcg agatagagag atgcatgcgg aatatagtaa tcgacaattc cgaacgtgaa
    18241 gcaaatccct gcgtaaaccg aaatcccccc aacgagaggt atcaatccct ggtgacgttt
    18301 gcggaagttt ggtttatcca ctaaaccgac tttttttgcc accttacggg caaaaaacag
    18361 aaacagtgtc gtgaataaaa aaatactgat gagatcagta ctcactgtca gtaaattcac
    18421 aatgcatgct ctcagagaaa attattagca gaagtataac cacgaagacc tttattcaga
    18481 agggaaagtc cgttccgaac ccaccagtcc tgtgcaaaaa ttaaccattt gatatgcaaa
    18541 ttgcataaca cgccagcaaa ttccaggaat tttcctaaca tcgccagcgc ggcattaaga
    18601 ttacagatat aaaagcaaaa cgccacgtaa acacgtggcg tttttggcat aagacaaatt
    18661 tatgagcgtt tcatcatttc gaagaaatcg tcattggtct tggtcatggc cagtttatta
    18721 atgaggaatt ccattgcatc gatttcgccc atcgggtgaa tgattttgcg caggatccac
    18781 attttctgca gttcttcctg agtagtgagc agttcttctt tacgggtacc ggaacggttg
    18841 tagtcgatag ccgggaagac gcgtttttca gcgatcttac gagagaggtg cagttccatg
    18901 ttgcctgtac ctttaaactc ttcgtagata acttcgtcca ttttagaacc ggtatcgata
    18961 agcgccgtcg cgataatagt caggctgccg ccctcttcca cgttacgcgc cgcaccaaag
    19021 aagcgtttcg gacgatgcag ggcgttggca tccacaccac cggtcaacac tttacctgac
    19081 gccggaacaa cggtgttgta agcgcgcgcc agacgagtga tggagtcgag cagaatgata
    19141 acgtctttct tgtgctcaac caggcgtttg gccttctcga tcaccatttc cgcaacctga
    19201 acgtggcgag atgcgggttc gtcaaaggta gaagcaacaa cttcaccttt taccagacgc
    19261 tgcatctcgg ttacttcttc cggacgttcg tcgatcagca gaaccatcag cacacaatcc
    19321 gggtggttgt aagcaatgct ctgggcaatg ttctgcagca gcatggtttt accggctttc
    19381 ggcggtgcca caatcagacc acgctgacca cgaccgatag gtgatgccag atccagtacg
    19441 cgagcagtta aatcttcagt agaaccgtta ccacgttcca tacgcagacg agaatttgcg
    19501 tgcagcgggg ttaagttctc aaagaggatt ttgttgcggg cgttttcagg tttgtcgaag
    19561 ttaacttcgt taactttcag cagcgcaaaa tagcgttcac cttctttcgg cgggcgaatc
    19621 ttaccagaga tggtatcacc agtgcggagg ttgaaacggc ggatttggct aggggaaacg
    19681 tagatgtcat caggaccggc gaggtaggag ctgtctgcgg aacggaggaa accaaatcca
    19741 tcctgcaata tctccagtac gccatcacca aagatatctt cgccactctt tgcgtgctgc
    19801 ttcaggatgg caaaaataat gtcctgctta cgcatgcgag ccaggttttc cagccccata
    19861 ttttcgccga gagtgatcag ctcagaaacc ggcgtattct ttaattcggt aagattcata
    19921 gtggtgtgag ttcttaaact tggggtaaat ctcgaactta atgttgtgaa tggtatggca
    19981 ggatcatcca tgcctgttta cggtcatcga ctcatgtctt ttcgctgcct ggtcacagga
    20041 gagtacgcag aactgaaacg acaagacgga ttgagtgacg agccagaaat ctgttcactt
    20101 cgcatttaag ataaaacggg aagcgttgga aattacaaga ttcaaactta ataaggtatg
    20161 tttaatacga agtcaacact aagttagcat gactcacgcc gggcgtccag tttttagcga
    20221 cggggcaccc gaacatgaaa ttcccttacg ccaggttagc gtcgaggaac tctttcaact
    20281 gacctttaga cagtgcgccc actttggttg ccgccacttc accgtttttg aacagcagca
    20341 gagtcgggat accacggatg ccatatttcg gcgcagtgcc agggttttga tcgatgttca
    20401 gttttgcaac ggtcagtttg ccctgatatt cgtcagcgat ttcatccaga atcggggcga
    20461 tcattttgca cggaccgcac cactctgccc agaaatcgac gaggatcgcc ccgtccgctt
    20521 tgagtacatc cgtgtcaaaa ctgtcgtcag tcaggtgaat aattttatcg ctcatatata
    20581 actccacagg aataagcctg gcgtgttggt ttcgttgttg gtgtaacatt aaccaactaa
    20641 aggttgactt tatttcaccg gatacgcttt cgtaaagcaa tagtaagctg atattctacc
    20701 acactatgag caaaacacat ttaacagaac agaagttttc cgacttcgcc ctgcatccga
    20761 aggttgtaga agcccttgaa aaaaaagggt ttcataactg tacgcccatt caggcactgg
    20821 cccttccgct gacgctggcg ggtcgtgacg tagccgggca ggcgcaaacc ggtaccggga
    20881 aaacgatggc gtttcttacg tcaacgtttc attatcttct ctctcatcct gcgattgccg
    20941 atcgcaaggt gaatcaaccg cgtgccttaa ttatggcacc gacgcgtgaa cttgccgtgc
    21001 agatccatgc cgacgcagaa ccgctggcgg aagctactgg cctgaagctg ggtctggctt
    21061 acggtggtga tggctacgac aaacagctga aagtgctgga aagcggcgtt gacattctga
    21121 ttggcaccac gggtcgttta attgactacg ccaagcagaa ccacattaac ctcggtgcca
    21181 ttcaggtagt ggttctggat gaagccgatc gcatgtacga tctgggcttt attaaagata
    21241 tccgctggct gttccgccgt atgccgcctg caaaccagcg cctcaacatg ctgttctccg
    21301 ccacgctttc gtaccgggta cgtgaactgg cgttcgagca gatgaacaat gccgaatata
    21361 ttgaagtgga accggaacag aaaacgggcc accgtattaa agaagagctt ttctaccctt
    21421 ctaacgaaga aaaaatgcgt ttgctgcaaa cgctgatcga agaagagtgg ccagacagag
    21481 cgattatttt cgccaatacc aaacaccgtt gtgaagagat ctggggccac ctggcggcag
    21541 atggtcatcg tgtcggtttg ttgacaggcg atgtcgcgca gaaaaaacgt ctgcgtattc
    21601 ttgatgaatt tacccgtggc gatctggata ttctggttgc caccgacgtt gccgcgcgtg
    21661 gtttgcatat tccggcagtg acgcacgtct ttaactacga tttacccgat gactgtgaag
    21721 attacgttca ccgtattggt cgtacaggtc gcgcaggcgc aagcggtcac tctatcagcc
    21781 tggcgtgtga agagtatgca ttgaatttgc ctgctattga aacctatatt ggtcactcaa
    21841 ttccggtaag caaatacaat ccggacgcat tgatgaccga tctgccaaaa ccgctgcgcc
    21901 tcacgcgccc acgtacaggc aatggtccgc gtcgtactgg cgctccgcgt aatcgtcgtc
    21961 gttcaggtta aataaaatgt catactcaaa atcattcaaa ctgtatcaag atggcaagag
    22021 aatgaatccc gatgagcata ctgtagtaag tgattcaggt gaaagaatgc agccaacaca
    22081 gagacagatt gaaggatgaa gagtatgggt tccacctcgt cgctgtatgc agccattgat
    22141 ctcggttcga atagttttca tatgctggtt gtgcgcgagg tggctggaag catccagacg
    22201 ctgacgcgaa taaaacgcaa agtgcgtctg gctgctggcc tgaatagcga aaatgccctg
    22261 tctaatgaag caatggagcg cggttggcaa tgtctgcgcc tgtttgctga acgtctgcaa
    22321 gatatccctc cctcgcaaat tcgcgttgtc gctacggcga cattacgtct tgccgtcaat
    22381 gcgggtgatt ttattgccaa tgcgcaggaa atcctcggtt gtccggtaca ggtgatcagc
    22441 ggtgaagagg aagcacgtct gatttatcag ggcgttgctc acacaactgg cggtgccgat
    22501 cagcgcctgg tggtggatat aggcggtgcc agtactgaac tggtaaccgg cacgggtgca
    22561 caaaccacct cgttgttcag cctgtcgatg ggctgcgtca cctggctgga acgctatttt
    22621 gccgatcgta atctggggca ggaaaatttt gatgctgcag aaaaagcggc acgcgaagtg
    22681 ttacgtccgg ttgccgatga attacggtat cacggctgga aagtgtgcgt tggtgcttcc
    22741 ggcaccgtgc aggcgttaca ggaaatcatg atggcacagg ggatggatga acgcattacc
    22801 ctggaaaagt tgcagcaatt gaaacagcga gccattcatt gcggtcggct ggaggaactg
    22861 gagattgacg ggctgacgct ggaacgtgcg ttagtgttcc cgagtggtct ggcgatcctg
    22921 atcgccattt ttaccgaact gaatattcag tgtatgaccc tggcgggcgg tgcgctgcgt
    22981 gaaggcctgg tctacggcat gttgcatctg gccgtcgagc aggatattcg cagccgtacg
    23041 ctgcgtaata ttcagcgccg ctttatgatc gatattgatc aggcgcagcg cgtagccaaa
    23101 gttgcggcta acttcttcga tcaggtggaa aaggaatggc atcttgaagc aataagccgc
    23161 gatttgctca tcagcgcctg tcagcttcat gaaatcggcc tgagcgttga ctttaaacaa
    23221 gcgccgcaac acgctgctta tctggtgcgt aatctggatc ttcccggttt tacccccgca
    23281 cagaaaaaat tgctggcgac gctactgctc aaccagacta atccggtcga tctctcatcg
    23341 ctgcatcagc aaaatgccgt accgccgcgc gtcgcagaac aactctgccg tttactccgc
    23401 ctggccatca tttttgccag ccgtcgccgt gacgatctcg tgccagagat gacattacag
    23461 gctaaccatg aactgttgac cttgacgctt ccgcaaggtt ggctaaccca acatccgctg
    23521 ggtaaagaga ttattgctca ggaaagccag tggcagagct atgtccactg gccgctggaa
    23581 gtgcattaat gagtaagtgc cggatgcgat gctgacgcat cttttccggc cttgattatt
    23641 tccctcgttt tgccgccatc atcgctttca gattcgccag atggctttgc cctttctgca
    23701 tccgttcttc ggcgctgacc actttgcgct cctgttccca aatcagatca tcctgcggca
    23761 gctccagcaa aaagcggctc ggctccgggc gcaccagctc gccgtactga cggcgttctt
    23821 tacacagcgt aaaggtcaat tccttctggg cgcgggtaat gccgacatag gccagccgcc
    23881 gctcctcatc gatattatct tcatcgatgc tgctctggtg cggcaaaaac ccttcttcca
    23941 taccgaccat gtagacataa ggaaactcca gcccttttga cgcgtgcaga gtcatcagtt
    24001 gcacctgatc cagctcttct tcactctcac cacgctccat catgtcgcgc aaagtaaagc
    24061 gcgtcaccac ctgggtgagc gtcatcggct catccagttc gctgccttcc agcatctccg
    24121 tcatccagct aaacagttgg ttgacgttct tcatgcgcat ttcggcggct ttcgggctgg
    24181 gcgatgtttc gtacagccag gattcataat ccatgccgtg gatcagatcg cgcaccgcgg
    24241 caatcggctc ccgctccgcc agacgctgga tttctgccaa ccagtgagta aagcgggtca
    24301 atgcttcata accacgtccg ctaagcgtct gactcaggcc catatcaaag ctggcggtaa
    24361 acatgctttt attgcgcgtc atcgcccact cacccagctt tttcagcgtc gccgggccaa
    24421 tctctctctt cggcgtgtta acgatacgca gaaatgcgct gtcatcgtcc gggttagtca
    24481 gcacgcgcag ataagccagc aagtccttga tttcaggacg agagaaaaac gacgtaccac
    24541 cagaaatttt gtacgggatg cggttttgca tcaggaattt ttcaaacact cgcgactgat
    24601 ggttaccgcg ataaagaata gcgtaatctt tgtactgcgt tttattgacg aagtgatgcg
    24661 caatcagttc accagtgacg cgctcggctt catgttcttc gttattcgcg cttaaaactt
    24721 taagctctgt gccgtaaccc agttcggaga acagacgctt ctcaaagacg tgcgggttat
    24781 tggcgatcag gatgttcgcc gctttcaaaa tacgtccgga agagcggtag ttctgctcaa
    24841 gcttaatcac cttcagcgcc ggaaaatcct gactcagcag caccaggttt tgtggacgtg
    24901 cgccgcgcca ggagtagatc gactggtcgt catcgcccac cacggtaaag cgcgcgcggc
    24961 tgcccaccag cagtttcacc agttcatact ggctggtgtt ggtgtcctga tactcatcca
    25021 ccagcagata gcgaattttg ttctgccagc gctcgcggac ttcttcattg cgttgcagca
    25081 gcaacgtcgg caataaaatc agatcatcga agtcgagaac attgcacgct ttcaggtgtg
    25141 catcatacag cccataacaa tgggcaaaaa tacggtcccg ctcgccaatc gcactggctg
    25201 ccgcctggga cggtgttttg agatcgttct tccagttaga gatggtcgaa atcaactgtt
    25261 gcaggagaac tttgtcatct tcaatcagcc cttcggtcaa ctctttgagc aacgccagct
    25321 gatcggtatc gtcaaacagc gaaaagttcg ccttcatccc cagcgccgca tactcacgct
    25381 tgatgatatc cagccccagc gtgtggaagg ttgagatcat cagcccacgc gcctctttgc
    25441 gacccagcgt ctgtccgaca cgctctttca tctcgcgcgc cgccttattg gtaaaggtca
    25501 ccgccgcgat atgtcgcgcc tgataaccgc aaccgcggat cagatgggcg attttattgg
    25561 tgatcacacg agttttaccg gaacccgcgc ccgccagcac caggcagggg ccggtaacga
    25621 attcgacagc ttgttgttgg ccggggttta gacgcatagg tgtattgctc aatcttcgaa
    25681 cggggggagg attgtagcag aatcgcattt gaatgtgacc gcgtgtaagc cgctaaccga
    25741 ttggctgcgg atgtttaaat cagagaggga agaccgaaac cggagttcat cgtgaatcca
    25801 ttagacttag aaaatatcgg gtcgtaacgt tgtgttatta ctttttcaga gtttactaag
    25861 gagaggtgta ctcgtggcga ttaatttcag tcctaaggtt ggtgaaatac tggattgcaa
    25921 ctttggtaac tatcctgtta gccagaatgg acagttcagc accacgtatt acgatggccg
    25981 cattcctcct gaaatgataa aaaacaggtt agt
//
LOCUS       DAJVQG010000045        25901 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJVQG010000045 DAJVQG010000000
VERSION     DAJVQG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25901
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.045"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..965)
                     /locus_tag="OQM83_001683"
     CDS             complement(<1..965)
                     /locus_tag="OQM83_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1519965.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNT"
     gene            complement(1025..1576)
                     /locus_tag="OQM83_001684"
     CDS             complement(1025..1576)
                     /locus_tag="OQM83_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HCS1519966.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            complement(1859..2149)
                     /gene="ubiK"
                     /locus_tag="OQM83_001685"
     CDS             complement(1859..2149)
                     /gene="ubiK"
                     /locus_tag="OQM83_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCS1519967.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
     regulatory      2271..2419
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            2523..3176
                     /gene="ribB"
                     /locus_tag="OQM83_001686"
     CDS             2523..3176
                     /gene="ribB"
                     /locus_tag="OQM83_001686"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCS1519968.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     gene            3437..3607
                     /locus_tag="OQM83_001687"
     CDS             3437..3607
                     /locus_tag="OQM83_001687"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1519969.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(3665..4438)
                     /gene="zupT"
                     /locus_tag="OQM83_001688"
     CDS             complement(3665..4438)
                     /gene="zupT"
                     /locus_tag="OQM83_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCS1519970.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            4581..5369
                     /gene="ygiD"
                     /locus_tag="OQM83_001689"
     CDS             4581..5369
                     /gene="ygiD"
                     /locus_tag="OQM83_001689"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCS1519971.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(5407..6567)
                     /locus_tag="OQM83_001690"
     CDS             complement(5407..6567)
                     /locus_tag="OQM83_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCS1519972.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(6573..7244)
                     /locus_tag="OQM83_001691"
     CDS             complement(6573..7244)
                     /locus_tag="OQM83_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCS1519973.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(7392..8873)
                     /gene="tolC"
                     /locus_tag="OQM83_001692"
     CDS             complement(7392..8873)
                     /gene="tolC"
                     /locus_tag="OQM83_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCS1519974.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            9078..9707
                     /gene="nudF"
                     /locus_tag="OQM83_001693"
     CDS             9078..9707
                     /gene="nudF"
                     /locus_tag="OQM83_001693"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCS1519975.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            9708..10130
                     /locus_tag="OQM83_001694"
     CDS             9708..10130
                     /locus_tag="OQM83_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCS1519976.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            10155..10982
                     /gene="cpdA"
                     /locus_tag="OQM83_001695"
     CDS             10155..10982
                     /gene="cpdA"
                     /locus_tag="OQM83_001695"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCS1519977.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            10982..11563
                     /gene="yqiA"
                     /locus_tag="OQM83_001696"
     CDS             10982..11563
                     /gene="yqiA"
                     /locus_tag="OQM83_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCS1519978.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            11592..13484
                     /gene="parE"
                     /locus_tag="OQM83_001697"
     CDS             11592..13484
                     /gene="parE"
                     /locus_tag="OQM83_001697"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCS1519979.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPNSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(13532..13846)
                     /locus_tag="OQM83_001698"
     CDS             complement(13532..13846)
                     /locus_tag="OQM83_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCS1519980.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(13877..14458)
                     /gene="mdaB"
                     /locus_tag="OQM83_001699"
     CDS             complement(13877..14458)
                     /gene="mdaB"
                     /locus_tag="OQM83_001699"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCS1519981.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            complement(14568..15917)
                     /gene="qseC"
                     /locus_tag="OQM83_001700"
     CDS             complement(14568..15917)
                     /gene="qseC"
                     /locus_tag="OQM83_001700"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCS1519982.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(15914..16573)
                     /gene="qseB"
                     /locus_tag="OQM83_001701"
     CDS             complement(15914..16573)
                     /gene="qseB"
                     /locus_tag="OQM83_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCS1519983.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            16725..17117
                     /gene="ygiW"
                     /locus_tag="OQM83_001702"
     CDS             16725..17117
                     /gene="ygiW"
                     /locus_tag="OQM83_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCS1519984.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            17170..17652
                     /locus_tag="OQM83_001703"
     CDS             17170..17652
                     /locus_tag="OQM83_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1519985.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            18198..20456
                     /gene="parC"
                     /locus_tag="OQM83_001704"
     CDS             18198..20456
                     /gene="parC"
                     /locus_tag="OQM83_001704"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCS1519986.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKVAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            20689..21426
                     /gene="plsC"
                     /locus_tag="OQM83_001705"
                     /gene_synonym="parF"
     CDS             20689..21426
                     /gene="plsC"
                     /locus_tag="OQM83_001705"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCS1519987.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            21501..22913
                     /gene="ftsP"
                     /locus_tag="OQM83_001706"
     CDS             21501..22913
                     /gene="ftsP"
                     /locus_tag="OQM83_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCS1519988.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            23024..25243
                     /locus_tag="OQM83_001707"
     CDS             23024..25243
                     /locus_tag="OQM83_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCS1519989.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINHYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSPRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLHCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(25286..25543)
                     /gene="yqhH"
                     /locus_tag="OQM83_001708"
     CDS             complement(25286..25543)
                     /gene="yqhH"
                     /locus_tag="OQM83_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCS1519990.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(25594..>25901)
                     /locus_tag="OQM83_001709"
     CDS             complement(25594..>25901)
                     /locus_tag="OQM83_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1519991.1"
                     /translation="FKTANNPDEDMQIERQFIYDNPICFGEESLFSRVDEIRVLEKTA
                     DSARIHVRFTLTNGNNEEQELVLQRREGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
BASE COUNT         6150 a         6399 c         6840 g         6512 t
ORIGIN      
        1 gtattgaata aattagtaat agcaaatggg ccaggaggaa cattttcttg ataaataata
       61 cggttgttct gtgagacagt gacctttgca ttcgtttgtg caacacccgt gatttgcggt
      121 gcgtaaccac gtagatcagg cggcaacata ttctcatcgc ttttcaacga aacgccagaa
      181 aaatggaaag agtcgaaaat agaggattca gtatcgtatt gacctatagt tagttttgca
      241 ttaattgctg ggattgggcg aaacaaataa atacgcggaa aatccaggtt gttcgcctgg
      301 cttttgccat tgttaaatga ctgatcgtac tggtaatcag tacgcaggcg ccaggcccca
      361 agattaaccc cagcctgccc ataggaactt atattttgtg aattatcgcc ctgatgtggt
      421 gcataatgag aggcatataa attgtagtca agaaatgccc cagcaatacc atgatcccaa
      481 agttcaggag gcgtccagtt tgcatctttg tattttaacc atgcctgagg tgcagatatg
      541 gataattgca ttttcccttt atcgagataa gttgtaattt gtttttcttt ttcgataggg
      601 taacaaccat cgatcaggtt ttgctgagcc tcttcaataa tctcatcagt aaagcctaat
      661 aatgttaatt gctctggagt cagacaaact gcagtttggt tatcaattaa tacccattgc
      721 aggtccagat ggattggcgt ccttttatta ttaataagag cgtttaatga gtatttacct
      781 acgggtaatt ggccatcagt ttcaaattgg gaaaggttaa cgttttcacg atcttcggct
      841 tcgattaaat ctttgttgaa ttcaacggca taagctgacg cacaaccaat gatgagaacg
      901 gataatgaac aataaatatt tttaatcaat tcgcttattg tggttaattt taatttttta
      961 tacatctgtt ccacttatca ttaatccgtt aatgatgtgg ccgggcgaac ccggcctaat
     1021 gtgattactc gtaagcaatc ttatagtcag cctgagcgta aacgttgccc ggggtagcat
     1081 ctttgcccgg ggattccata cgagctttga agttcagttc ctgatagctg ttagtattgt
     1141 tgaatgtaat tactaccgga gtacctaaag tcacttcgcc gctctggcta tccagaatac
     1201 gtacgccaac gcctgtggca ccaccgattt cagtttcttt ggtgtttttc agcaggtcag
     1261 tctgacccgt catagtattc gctgtggtga aagtaacctt cgcttttgaa taagtggttc
     1321 caccctggct caacatgcag tcttgcaggt gaatagtgaa aggctcagcc aaagatttct
     1381 ggtcgctttt cagaacggta tctgctactt cacccagatt tacagttaag tcttcgtcac
     1441 ctggtttaat tgaacatgga gcagagataa cttcaccttt aaatttaaca atgccgtgac
     1501 cttcgtcagc aaatacgtta ttggaaagaa tcattgctac gccagcaaca agaagtgact
     1561 ttttaaaagc agacataata ttcctttata taaatataag tagaaaatga atgattagta
     1621 actgttattt aaggattgct aatcattcaa atattatatt ttacttttcg ggttttatga
     1681 aattagttta tctaaatatg gattaagtaa aacttaaaaa atacataaca attatatttt
     1741 caattgcact cattcatttt tgtgatgata ttctgaatgg aattaagcga taaggtaata
     1801 gaaagcactg taaattagaa gattaattca taatttacag tgcattaaag acaaatgttt
     1861 acagcgttgg ggggagagtc tctggatctg gctgcttttt aatctcagta ctgcgatttt
     1921 ccagttcgct cattctttgt tcaagccgcg ccagtttctc acgagtccgt aacaggactt
     1981 gcgtttggac gtcgaactct tcacggctta caaggtcgag gcgcgtcagc tgagcttgta
     2041 gggtttggcg gatttttttc tccacatctt ccccgaactc cctgattcct ttaggcattg
     2101 attcgtgaac ctggcgagcg atttgctcaa tttttttcgg gtcaatcatt gtagtttccc
     2161 tgtactgata ggtgttgagt accattgtag tgcgataagg gtaagtcata aaccagaatt
     2221 atgtgaagct atgcgttgct gccgctaatc attagcgtta tagtgaatcc gcttattctc
     2281 agggcggggc gaaattcccc accggcggta aatcaactca gttgaaagcc cgcgagcgct
     2341 ttgggtgcga actcaaagga cagcagatcc ggtgtaattc cggggccgac ggttagagtc
     2401 cggatgggag agagtaacga ttctgtcggg catggacccg ctcacgttat tttggctata
     2461 tgccgccact cctaagactg ccctgattct ggtaaccata attttagtga ggttttttta
     2521 ccatgaatca gacgctactt tcctcttttg gtacgccttt cgaacgtgtt gaaaatgcac
     2581 tggctgcgct gcgtgaagga cgcggtgtaa tggtgcttga tgatgaagac cgtgaaaacg
     2641 aaggcgatat gatcttcccg gcagaaacca tgaccgttga gcagatggcg ctgaccattc
     2701 gccacggtag cggtattgtt tgcctgtgca tcactgaaga tcgccgtaaa caacttgatc
     2761 tgccaatgat ggtagaaaat aacaccagcg cctatggcac cggttttacc gtgaccattg
     2821 aagcggctga aggtgtgact accggtgttt ctgccgctga tcgtattacg accgttcgcg
     2881 cagcgattgc cgatggcgca aaaccttctg atctgaatcg cccgggccac gttttcccac
     2941 ttcgcgcgca ggcaggtggt gtactgacgc gtggcggtca tactgaagcg actattgatt
     3001 tgatgacgct ggcaggcttt aaaccggctg gtgtgctgtg tgagctgact aatgacgatg
     3061 gcacgatggc gcgtgcacca gagtgcattg agtttgccaa taaacacaat atggcgctcg
     3121 tgactattga agacctggtg gcataccgtc aggcacatga gcgtaaagcc agctgaaaac
     3181 cgctgcttaa ttactgcctt aatcaagaaa ccgaagttgt agctggcttc ggtttttatt
     3241 ttgccctgct atgcaagatg ttaattccgc ttctctttct atgagaaaat ttcattaata
     3301 tcaggcattc tttttcatta tgatcaaaat catataattg ttccgttatt atgcgaccat
     3361 atgaatatat ccgaaaaagg atatgtgaaa tttatgcgct aaatgtttag tattgttaaa
     3421 tattaagggt aaaaggatgt ttatcgcctg gtactggatt gtattgattg ctctggttgt
     3481 ggtgggttat ttcctgcatt tgaaacgtta ttgtcgggcg tttcgccagg acagagacgc
     3541 actgcttgaa gcgcggaaca aatacttaaa cagtacgaga gaagagacag ccgaaaaggt
     3601 agaataaggc tttcgtgaag ctggcagaag ttgtccatgt gctggacaac ttctgatgtt
     3661 gtttttaacc aattcccgcc gtttgtagca gcactaaact gaatcccatc actgacattc
     3721 cacatagtac gccatagctg gggttattat tagggtcaat ctctttggcg agcggcatta
     3781 attcatcaac cgagagcgcc accataattc ctgcaaccgc cgccatgatt gccgccatga
     3841 ccaccgggga aatcatgcta ccgaggatta accacgccag cacaccacca agaatttctg
     3901 ctagtccaga aatccccgcc cacagaatcg cggtgcgttt agaccccgtt gccgcataaa
     3961 ccgggcctgc cactgccaga ccttcaggga tattgtgcaa cgcgacggcc agtgcgatgc
     4021 caaatcccag ctccaggttg ctgctcgccg tgacaaaggt ggcaatccct tccgggaagt
     4081 tatgcagact gatgccgaga gtgagcagaa tggctgtgcg cttgatcgat tttggcaacg
     4141 gctgcaccga tttttgcatt aaatcctgcg gatgcgcatg tggcaacatg cggtccaggc
     4201 caaaatagcc aagcagaccg aagataaaca ttccataacc caacacaggc gacattcctt
     4261 cagctgctag tgcggcagga agcatttcca ttaatgagat gagcaacatg atccccgccg
     4321 caaatcctag cgaaaacgcc agtaagcggt tcgagggttt ttgcccgaga acgccgagaa
     4381 acgcgccaat aaacgtggct gcccccgcca gtatggtcag aatgagaggt actgacatcg
     4441 acaactcctt ataaatcttc accttcaggc agatcgtcaa aataactgat gatcatcatc
     4501 attgttatcc gggttcttac tccgccgaac aagcgtattg tgaggatatc aaaatgacac
     4561 ctttagttaa ggatatcatc atgtcttcaa cacgtatgcc agcattgttt ttaggtcacg
     4621 gtagtccgat gaacgtgctg gaagataatt tgtatacccg cagctggcag aagttgggga
     4681 tgacattgcc acgcccgcaa gcgattgtgg tggtttcggc tcactggttt acccgtggaa
     4741 caggagtgac cgcgatggag acgccgccca cgattcatga ctttggtggc ttcccgcagg
     4801 cgctgtacga tacgcattat cctgctccgg gttcgcctgt gctggcacag catctggttg
     4861 agctgttagc gtcggtcccg gtgacgctgg ataaagaagc ctggggcttt gaccacggtt
     4921 cctggggcgt gctgattaag atgtatcctg acgccgatat cccgatggtg cagttgagta
     4981 tcgacagtag caaacctgcc gcctggcatt tcgaaatggg gcgcaaactg gcagcgctgc
     5041 gagatgaagg aataatgttg gtcgccagtg gcaacgtggt gcataacctg cgtacagtga
     5101 agtggcacgg tgatagttca ccatatcctt gggcgatgtc gtttaatgag tatgtgaaag
     5161 cgaatctgac gtggcaaggg gcggtagagc agcatcctct ggtgaattac cttgaccatg
     5221 aaggtggcgc gttatcgaac ccaacgccgg agcactatct gccgttgttg tatgtcttag
     5281 gtgcgtggga tgggcaggag ccaattacca ttccggtcga tggtatagaa atgggcagcc
     5341 tgagtatgct gtcggtgcag ataggttaac aattaccgga tggcgaatgc catccggcat
     5401 cattgattac tcaataaaaa tatgtggata aaaccgagac atatcctggg tgatcaacgc
     5461 acggtcttca cgaatgccaa ttccggcagg ttgatcgttc accagccagc taccaatcag
     5521 catatagctg tcgccgaatt tcggtaacgg gtggaattgc tgaacaatca tcccttcttc
     5581 gccatacgga ccttccgctg cttcaatggt tttgccgttc tcaatgatcg acacgtttgc
     5641 gccttcacgg gagaagatcg gtttaaccac atatttttcc atttgcggat gatcatcttc
     5701 cgcaaaataa gcgggcagca ggttcgggtg attcgggaac atctcccata gtagcggtag
     5761 aagcgctttg ttggagataa tgctcttcca cgccggttcc agccagcgta cgcctgcatc
     5821 ctccagcttg gtggagaaca tctcacgcaa cataaattcc catggataca gcttgaacag
     5881 gttggaaatt acctgatcct gtaaatccgt gaactgacct ttttcaccta acccgatatc
     5941 gtcaatgtag aggaactcgg tagcgatttc agcttccgtc gcgcagtcct gcaaatactg
     6001 aatggttccg cgatcttcca ccgtgtcgcg acagcaggtg agatgcagca actggaagcc
     6061 atactgttca cgcaactcga caaagcgatc gattagcttt tcttgcagac tgttaaactg
     6121 gtcgctgccc tccggcaagt taccggcgtt aagctgatct tccagccaga tccactgaaa
     6181 gaacgccgcc tcgtatagtg acgttggtgt atcggcgtta ttttccagca gtttaggttc
     6241 gccagtgcca tcccatgcca gatcaagacg cgaataaagc gatggctggt gagtcagcca
     6301 tgactgacgc acaaaactcc aggtgcgttt tggaatgcgg aatttggtca tcagctcatc
     6361 gctggcgatc actttttcca ccactttcag gcacatctgg tgcagttcgg cggtgacttc
     6421 ttccagcttt tcaacctggg cgagggtcaa cttgtagtaa gcatcttcac accagtacgg
     6481 ctcgccgtac atggtgtgaa aattgaaacc gtattcgtgg gctttttcgc gccagtccgg
     6541 gcgctcggta atactgactc tttccatcgg tatcagccac ccattgaacg agaagaggtg
     6601 ccggttgcgc tacgctgcat agtgctttgt ttggcaacag attcaccaaa accgccacgg
     6661 gtaacggtag tagtggtcgc cggttttggt gccatggctg tcttcggtac ggtcatggtg
     6721 cggcctggct gggctgcgcc atagttttta cccgtcgcgt cggtatattt accgtaagcc
     6781 gggctggctg ggtttttcga ggagaacagc ggctgctgtg caaatcccgc gccgccgccc
     6841 atcagacgcc ccatcatgta accggccatc agcggcatcc agaaactccc gctggattgc
     6901 tgggcctgcg cctggttttc tggtgccatg ccagcctggg ctggtgcctg ctggcactga
     6961 ccttcaccaa attcagcaac acagtcttca cgggtggcgt atttcggcgc agtacgttcg
     7021 gcttctttca gcgcattgtt gtacgcggtg gtacattcgg cgcttttgcc tgggtttgca
     7081 gctgaacagt cgtcagcatt ttgatagaga gacactgttt catcactctt ttcacagcca
     7141 gccagcataa aaacagtggc aaccgcgaga gcgactggtg tcagatggcg tgcgctccag
     7201 tttttgcgga acgatgcgtg gcgtatggat tttgtccgtt tcatttttgt cttccgggac
     7261 cagtggtaaa tacccatcag aatagaggat ggctggtcga aattgaagcg agaaggggga
     7321 agaatgcggc agataacccg tatctttacg ttgccttacg ttcagacggg gccgaagccc
     7381 cgtcgtcgtc gtcagttacg gaaagggtta tgaccgttac tggtggtagt gcgtgcggat
     7441 gtttgctgaa cgactggtgc cgggctatca ggcgcataac catcagcaat agcattctgt
     7501 tccggcgttt gcggtgcaac gttttccgga ttagtggaaa ccggtttgct cagcgcattg
     7561 ttcagtgcca gcagatcctg ctcgttcaac gtacccagag ctgacttaat attcaactgg
     7621 ttaatcaggt agttataacg cgcatttgcc agctcttgct tagcgttgta cagcgtggtg
     7681 gttgcatcca acacatcaac aatggtacgc gtaccgaccg agtagcccgc ttccatcgcg
     7741 tctaatgagc tttgagcgga aactacggct tgtttgtagg cgttaatgct actgatagat
     7801 gcattaatgt tgttgaagga ggaacgtacg gtttgcacga cgctacgatg cgcgctttcc
     7861 agttgctcgc tggcaccaac aaagttgtac tgggcctgtt tcacctgcga gttaaccatt
     7921 ccgccctgat aaatcggcag cgagaagctc aggcccactt tgttctggcc catattgctg
     7981 tcgtcatact gggtaccagc ggcaccacgg gttttcgaac cgctataaga ggtgtcagaa
     8041 atcccggtag aagccgttaa atccagcgtc ggtaagtgac catcctgcgc ctggcgaatt
     8101 tgctcgcgcg ccaggtcctg gctcaagcgt gcctgtaaca gcgacaggtt gcgtttttcg
     8161 gcttctttca gcagcgcgtt aaccggctgt ggtttgtcgg ttttaaagtt ttcgacattc
     8221 agcgccgcca gttccggata gtagttaccg gtgatctggc gcagctgctc taccgcgtta
     8281 tcaaggttat tacgtgcggt cacttcgttc gccagcacgg tatcgtactg cgcgcgggcg
     8341 ttctgcacgt cggtgatcgc taccaggccc acgttaaaac gttgggtggt ttgatctaat
     8401 tgacggtaga tcgcttcttt ttgtgcctgt gtataggaaa gaacgtcaat agcattcaac
     8461 acgttgaaat aagcggtcgc ggtgttgagg atcaaggttt gctgatcggt ctgatatgtg
     8521 acgtcctgaa tccctgctgc tttttcctgc agcgttaacg cacgccattt cgacatatca
     8581 aaaatggatt gagttaactg cagggacgca ctggtcgcgt tagagttgat gccgttcgcg
     8641 tcgcggtagc cgttgctata ggtgtaatct gcacctaaac ctagctgtgg cagtaatgga
     8701 ctgcgcgctt cattaatttt ttcaaaggca gcatcacgat cggcggcaga cttacgcaat
     8761 tccgggttac taaggcgtgc ttgctgataa acttgcatca ggttctcggc ctggctcaac
     8821 gaactgaacc cagaaaggct caggccgata agaatgggga gcaatttctt catttgcatt
     8881 ccttgtggtg aagcagtatt tagcgcgatc aaactgtaaa attattgcgg attctagcag
     8941 aagccgctac cgcaaaaggt tggcgttacg tgccataggg cgttaatgtg cctcaattta
     9001 acacatgact gctcaaacgg cagtcaaacg tcgctgaaat tcacatttaa ttcactatta
     9061 gtgccaggac attaacaatg cttaagccag acaacctgcc cgttacattt ggcaaaaacg
     9121 atgtagaaat tattgcacga gaaacacttt atcgcggctt tttttcatta gatctttata
     9181 gatttcgtca tcgtctattc aacgggcaaa tgagtcatga ggtacggcgg gaaatttttg
     9241 agcgcggtca cgccgcagtc ttgctaccct ttgacccagt gcgcgatgaa gttgtgctga
     9301 ttgagcagat tcggattgcc gcatacgaca ccagtgaaac cccctggcta ctggagatgg
     9361 ttgccgggat gatagaagag ggtgaaagtg tggaagatgt tgcccgtcgc gaagcgattg
     9421 aagaggcggg actgatagtc aaacggacca aaccggtgtt aagtttcctg gcaagcccgg
     9481 ggggcaccag tgagcgttcg tcaattatgg tgggcgaagt ggacgccacg accgcaagcg
     9541 gtattcatgg tctggctgat gaaaacgaag atattcgcgt tcatgtggta agccgggaac
     9601 aggcatacca gtgggtagaa gaggggaaaa tcgacaacgc agcgtcggtc atcgctttgc
     9661 aatggctgca gctgcatcat caagcgttaa aaaatgagtg ggcataaatg aagcgttaca
     9721 cacctgactt tcctgaaatg atgcgcctgt gcgagatgaa cttttcacaa ttgcgccgtt
     9781 tgttaccgcg caatgacgca cccggcgaaa ctgtaagcta tcaggtggca aacgcacaat
     9841 atcggctgac gattgtggaa tcgacccgat acactaccct ggtgacaata gaacagactg
     9901 cgcccgcgat cagttactgg agccttccgt caatgacggt gcgtctgtat catgacgcga
     9961 tggtggctga agtgtgttca agccagcaga tttttcgctt caaagcgcgg tatgattatc
    10021 ctaataaaaa gttgcatcaa cgcgacgaaa agcatcaaat taatcagttt ttagcggact
    10081 ggttgcgata ctgtttagca catggagcga tggcgattcc ggtttactag catcgtgaaa
    10141 cctaaggaca ccatttggaa agcctgttaa cccttcctct ggctggtgag gccagagtca
    10201 ggattttaca aattaccgac actcacctgt ttgcacaaaa gcacgaagct ctgttagggg
    10261 taaacacctg ggaaagctac caggcggtgc tggaggcgat tcgtccacat cagcacgaat
    10321 tcgacctgat tgtcgcgaca ggtgatttag cgcaggatca atcctctgcg gcctatcagc
    10381 atttcgctga aggcatcgca agttttcgtg cgccctgcgt ctggctgccg ggcaaccacg
    10441 atttccagcc cgcgatgtac agcgcgttac aggatgcggg tatctccccg gcgaagcggg
    10501 tgtttattgg tgagcaatgg caaatcctgt tgctggatag ccaggtgttt ggcgtgccgc
    10561 acggtgagct gagcgagttt cagcttgagt ggctggaacg taaactggcc gatgcgccag
    10621 aacgccatac gttgctgctg ctgcatcatc atccgctacc tgcgggttgt agttggctcg
    10681 atcaacacag tctgcgtaac gcgggcgaac tggataccgt gctggcgaag tttccgcacg
    10741 tcaaatactt gctgtgcggt catattcatc aggagctgga tctcgactgg aacggtcgcc
    10801 gcctgctggc aacgccgtcg acctgtgtgc agtttaagcc gcactgttcc aactttacgc
    10861 tggatactat cgcgcccggc tggcgtactc tcgagttaca tgctgatggc acgctgacca
    10921 ccgaggtgca tcgcctggcg gacacacgtt tccaacctga taccgcttca gaaggctact
    10981 gatgtctacg cttctttatt tacacggttt caacagctcg ccgcgctctg caaaagcgag
    11041 cttgttaaaa aactggctgg cggaacatca ccctgacgtt gagatgatca ttccgcagtt
    11101 gccgccgtat ccttccgacg cggcagaact gctggaatcc attgtcctgg aacatggcgg
    11161 cgattcgctg ggtattgtcg gttcgtcact ggggggatat tacgccacct ggttgtcaca
    11221 atgttttatg ctgcccgcag tggtggtaaa cccggcggtg cgcccgtttg aactgctgac
    11281 ggactatctc ggtcagaacg agaacccata caccgggcag caatatgtgc tagagtcacg
    11341 ccatatttac gatctcaaag tcatgcagat tgacccgctg gaagcgccgg atttgatctg
    11401 gctgctgcaa cagacgggag atgaagtgct ggattaccgc caggcggtgg cgtactacgc
    11461 ttcctgccgc cagactgtca tagaaggcgg caaccacgca ttcacgggct tcgaagatta
    11521 tttcaacccg atcgtcgatt ttcttggtct gcaccatctc tgacgatcaa ctcgaattgc
    11581 taacttaaac catgacgcaa acttataacg ctgatgccat tgaggtactc accgggcttg
    11641 agccggttcg ccgccgtccg gggatgtata ccgataccac tcgccctaac catttggggc
    11701 aagaagttat tgataacagt gtcgatgaag cactggcggg tcacgcaaaa cgcgtggatg
    11761 taatcttaca tgctgaccag tcgttagaag ttattgacga tgggcgcggg atgccggtgg
    11821 atattcaccc ggaagagggt gtcccggcgg ttgaattgat tctttgccgt ctgcatgcag
    11881 gcggtaaatt ctctaacaaa aattaccagt tctctggcgg cctgcatggc gtggggattt
    11941 cggtggttaa cgccctgtcg aagcgcgttg aagttaacgt gcgccgcgat ggtcaggttt
    12001 ataacatcgc ctttgaaaat ggcgaaaagg tgcaggattt acaggttgtc ggcacttgcg
    12061 gtaaacgcaa taccggtacc agcgtgcact tctggccgga tgaaaccttc tttgacagcc
    12121 cgcgattttc tgtttcacgc ctgacgcatg tgctgaaagc caaagcggta ctgtgccccg
    12181 gcgttgagat tacctttaaa gatgagatca acaacaccga acagcgctgg tgctatcagg
    12241 acggtctgaa tgattacctg gcggaagcgg taaacggttt accgacgctg ccagaaaaac
    12301 cgtttatcgg taatttcgct ggcgatactg aagctgtgga ctgggcgcta ctgtggctgc
    12361 cggaaggcgg tgaactgctg accgaaagct acgtcaacct gatcccaacg atgcagggcg
    12421 gtacccatgt taatggcctg cgtcagggcc tgttggacgc gatgcgtgag ttctgtgaat
    12481 atcgcaacat tctgccgcgc ggcgtgaagc tgtcggcgga agatatctgg gatcgctgcg
    12541 cctatgttct gtcggtgaaa atgcaggatc cgcagtttgc cgggcagacg aaagagcgtc
    12601 tctcttcgcg tcaatgcgcg gcattcgttt ctggcgtggt gaaagatgcc tttatcctgt
    12661 ggctgaacca gaacgttcag gcggcggagt tgctggcaga gatggcgatt tccagcgccc
    12721 agcgtcgtat gcgtgcggct aaaaaagtgg tgcgtaaaaa gctgaccagc ggcccggcgc
    12781 tgcctggcaa actggctgat tgtaccgcgc aggaccttaa ccgtaccgaa ctgttcctcg
    12841 tggaaggtga ctccgcaggc ggatctgcca agcaggcgcg cgatcgtgaa tatcaggcga
    12901 tcatgccgct gaaaggtaag atccttaaca cctgggaagt ttcttccgac gaagtgctgg
    12961 cctcgcagga agtgcatgat atttcggtag cgatcggtat cgatcctaac agcgacgatc
    13021 tgagccagct tcgttatggc aaaatctgta tcctcgcgga tgcggactct gatggtctgc
    13081 acattgccac gctgctctgc gctttgttcg taaaacactt ccgcgcgttg gtgaaacacg
    13141 gtcacgttta cgtcgcactg ccaccgctct accgtattga tctcgggaaa gaggtttatt
    13201 acgcgctgac ggaagaagag aaagagggcg tacttgagca attaaaacgc aagaaaggca
    13261 agccgaacgt ccagcgtttt aaaggtctgg gggaaatgaa cccgatgcaa ttgcgtgaaa
    13321 ccacgcttga tccgaacact cgccgtctgg tgcagttgac tatcgatgat gaagacgatc
    13381 agcgtactga cgcgatgatg gatatgctgc tggcgaagaa acgctcggaa gatcgccgca
    13441 actggttgca agagaaaggc gacatggcgg agattgaggt ttaaaggaaa gaacattgcc
    13501 cggtcaggat ggccgggcaa acaaggcagg attaaatccc tggctgcaga atacgaatat
    13561 tcatctccag cacgtcgcct tttacggctt cgctgtacgc cttcatgtgc ggggtttgca
    13621 gatgcgcttc aaggtgcgcg atgctttccc actgctcaat catcacgata gaatccggtg
    13681 ccatagactg gaaactcacg ccagcagcgc aatccaccat tggcgcatag ccgtggcaac
    13741 cttcttcttt cagtacggtt ggaacgattt tagcaaactg atccaatacc gcctgacggt
    13801 gatgttgacc aggacgagta cggatttctg cgattacggt aagcatggtt aactccttct
    13861 aaagcctgag ctctagttaa ccaaaaattt ccacaagatg cttgcgatat tcttcagtat
    13921 agcggggaac atcaggcatt tttatcacgt cattagcgat aaatgtcggc agcggttcca
    13981 tgccaaggaa ttggtttgct ttatggaacg gcagatacac accgtcgacg ccaacgccgt
    14041 ggaagaattg atctttttcg gtgaaggctt ccattggtgc gttccaggtc agagaaagca
    14101 tatatttttt gccctgtacc aggccgccgg aaccgtattt ttttgacgga tctttgcggg
    14161 tacggccatc gctggcatac agcgtcccgt gaccttcggt gaatacatca tcaatgtatt
    14221 ttttcactgt ccacggcgcg cccatccacc agcctggcat ctgccagatc accacatcag
    14281 cccagagaaa gttttgtact tccgctttga catcgtagtc gctgtcggcg cgaacgatgc
    14341 gaacatcatg cccgaggtcg cgcagtgtgc catccgcgac ttcggtcagg gtgtcgttca
    14401 gttgaccatt ggagtgggcg aattttttcg cgccgttgat aatcagggtg ttgctcattt
    14461 tttaacctca aggtgagacg tttgtcgact atagtacgtc acagagcggt acggtaaaat
    14521 tagcaaaatg tgcaaagtct tttgcgaatt tagcaaaagt cttaccctta ccagctcacc
    14581 ttcgcctcaa atccaccttg ttccgcattc ccaaattcaa cattcatgcc atgcaatttg
    14641 gcgattcgct ggacaatcga tagcccaagt ccactgccgg tagcggtttg tccggggggg
    14701 cgatagaagc gttcgccaat tcgcgccagt gcctctggtg tcacaccggg gccgttatcc
    14761 ctcacgatga aattatcggc attcagcgtg acgtccacca cgctgccctg tggactgtag
    14821 cgcactgcgt tatccagaag gtttcgcacc aacaaactta gcaatagcgg ttgtccagtg
    14881 cgtttgatgc tgtgggcatt gagtgtcagt cgcacgtcaa ttttcgcctg ctgcgccgtg
    14941 tggtaaatat ccatcaccga cgattgcagg agatcttcaa gcgggatctc cgcgacgtcc
    15001 tgaaggttat ccagtgagtc cagccgcgat agcgtgagca gttgatcaac cagacgagta
    15061 gcgcgatcga tcccggaatg taattggagc agtgcttttt tccgcgcctg cggatcatcg
    15121 tcagagagct gcgcaacttc ggtttgcact ttcagcgctg ttaacgggct acgtagttcg
    15181 tgagctgcat cggaggtaaa gcgtcgttct cgaaccatca tcgcatgtgt gcgggcgaac
    15241 agttgattta gcgactcaac cagtggtcgc acttcgctgg gtacgccagt cgcgtttagt
    15301 ggtttttccg agtcagggtc acgcatacgt agtgccagcg ccagtttgtt cagcggcgcg
    15361 agttcacgac ccagtagtac catcatgatg attaacataa tcggcagtgc gaccagccac
    15421 gggatcaatt gcccggcaac aatcgccagc gccatgtctt cacggtattc ccattcctgg
    15481 ccaacaacga tgcgatattt gccatcaggt gaggtcatcc agacaaaacg ccaaggatcg
    15541 tcttcaccga ccagttgccc gtcagcaaaa ccttcccgtt gatagctata gggaatatct
    15601 tctccgttat cgccatcatt aaggaccatt ctgccgtcgt gggtaaagat ggcaaaggtc
    15661 agcgcatcgt catcaacatg accgtgtttt aatttatttg gcgtctgtgc catgcgatcc
    15721 gccgcgttga tttcgttgag atcgagcgta cttaaccgct tggcaaacag catcagttgg
    15781 gtgtcgaaca attcatcgac gttatccgtt gtttgtttcc aggcgacaaa gctggaaagc
    15841 agccaggtca ccgaggccag aattaaaaag attagcgtca gcctgacgcg cagactaaga
    15901 cgttgggtaa atttcatttc tcacctaatg tgtagccaat accatgcacg gtacgaataa
    15961 aatcactacc gagtttgcgt cgcagatgat gcacatgcac ttcaacggca ttactggtga
    16021 cctcttcgtc ccaggtatac agtttctctt caatcagttt gcgcggcagt acccgaccag
    16081 cgttacgcat cagtaattcc agcagggcaa attcttttgg tttcagtgtt aagggttcgc
    16141 cagccagcgt ggcgatacgt ttgccggggt cgagcatgac gttaccgtgg cgcagctcgt
    16201 tgctggcctg gccgttggtt cggcgcatca gagcctccag cctggcggcg acttctatca
    16261 acgcaaaagg tttacacaga taatcgtcag ctcccagacg cagcccttct acacgttccg
    16321 ctaacgcatc acgcgcggtc aggatcagta ccggctcacg ctgacctttc tctcgccatt
    16381 cgcgcaaaat atcgcgacca tccatgcctg gtaaggttaa atccaggatc accgcatcat
    16441 aaggtgcgct ataaagcgcc tcttttccct gacgaccttg tgtaaaccag tcgacactaa
    16501 aacccatttt actaaggcca gttttgatgc cgtcgccaat cagcatgtca tcttctatca
    16561 gtaaaattcg cattttttca tccctgcgat aaccgtattt gctgccagta aaccgtgttg
    16621 taacgcgctc tgtacagcat taaattttat ttttttccct taagaagttg ttaaggacta
    16681 tcttgttaaa tgctcggaaa cagacattaa agggagtaat aaacatgaaa aaattcgcag
    16741 cagtaatcgc agtaatggcc ctgtgcagtg caccggtgat ggcagcagag cagggcggtt
    16801 tttctggccc atcggcaacg caaagtcagg ccggaggatt ccaggggccg aacggcagcg
    16861 taacgactgt agaaagcgca aaatccctgc gtgacgacac ctgggtaacc ctgcgcggca
    16921 atatcgttga acgcatctct gacgatctct acgtgtttaa agatgccagc ggtactatca
    16981 atgttgatat cgaccacaaa cgctggaacg gtgtgacggt gacgccgaaa gatacggttg
    17041 agattcaggg tgaagtcgat aaagactgga attctgttga aattgacgtc aaacagatcc
    17101 gcaaagtaaa tccgtaattg ttaccgctcc cgggacgcgt tcccgggaat aatttcgcag
    17161 ggaggcaaaa tgacaaacct gacactggat gtaaacatta tcgatttccc atcaatccct
    17221 gtggcgatgt tgccgcaccg ctgtagccct gagttgctca actacagtgt ggcgaaattt
    17281 atcatgtggc gtaaagagac ggggctttct cctgttaacc aaagccagac ttttggcgtc
    17341 gcctgggacg accctgccac caccgcaccg gaagcgtttc gctttgatat ctgcggcagc
    17401 gttagcgaac cgattcccga taatcgttat ggtgtgagca atggtgaact taccggtgga
    17461 cgttatgccg tggcccgcca cgttggcgag ctggacgata tttcacacac gatatggggc
    17521 attattcgcc actggctgcc tgcaagcggc gagaaaatgc gtaaagcacc gattctgttt
    17581 cactacacca atcttgccga aggggtgacg gagcagcgac tggaaacgga tgtttatgtg
    17641 ccgttagcgt gatggggaat ctttaccgga gtgtgagcca aatcactcgc gataagtttg
    17701 agcgaacaat gccacaggcg tattgttgtt gggcttataa tttctccggt aaggaatacc
    17761 atgtatacgc gaaatttatt atccggtgcg ccgtaagccg tctcgggggt acccatgagc
    17821 aagccgcact tatggcagga ggcgcggggc actggtggag acacagctaa actgggctga
    17881 accatatact tacagtcgac tactttgatg ctttaggctt ccctggactc gcataacctc
    17941 aacttctcga atcgcccgga gcggaccccc atgccgagtg gtgtggcatg ggcgcaacct
    18001 tgtgctgccc tctatgccga tcaagtctac agcaaagcgt tgtatatcaa ggcacattga
    18061 tgcctttatc gccgtcggag cacgtccggc ggcgctattc acgctgatct cctgtgactc
    18121 gacgcggcag ataatgtagt atctccggca atattgcccc tttgaaggct ggcgaatcag
    18181 ttgaggaatc agaattaatg agcgatatgg cagagcgcct tgcgctacat gaatttacgg
    18241 aaaacgccta cttaaactac tccatgtacg tgatcatgga ccgtgcgttg ccgtttattg
    18301 gtgatggtct gaaacctgtt cagcgccgca ttgtgtatgc gatgtctgaa ctgggcctga
    18361 atgccagcgc caaatttaaa aaatcggccc gtaccgtcgg tgacgtactg ggtaaatacc
    18421 atccgcacgg cgatagcgcc tgttatgaag cgatggtcct gatggcgcag ccgttctctt
    18481 accgttatcc gctggttgat ggtcagggga actggggcgc gccggacgat ccgaaatcgt
    18541 tcgcggcaat gcgttacacc gaatcccggt tgtcgaaata ttccgagctg ctattgagcg
    18601 agctggggca ggggacggct gactgggtgc caaacttcga cggcactttg caggagccga
    18661 aaatgctgcc tgctcgtctg ccaaacattt tgcttaacgg caccaccggt attgccgtcg
    18721 gcatggcgac cgatattcca ccgcataacc tgcgtgaagt agctcaggcg gcaatcgcat
    18781 taatcgacca gccgaaaacc acgctcgatc agctgctgga tatcgtgcag gggccggatt
    18841 atccgactga agcggaaatt atcacttcgc gcgccgagat ccgtaaaatc tacgagaacg
    18901 gacgtggttc agtacgtatg cgcgcggtgt ggaagaaaga agatggcgcg gtggttatca
    18961 gcgcattacc gcatcaggtt tcaggtgcgc gtgtactgga gcaaattgct gcgcaaatgc
    19021 gcaacaaaaa gctgccgatg gttgacgatc tgcgcgatga atctgaccac gagaacccga
    19081 cccgtctggt gattgtgccg cgttccaacc gcgtggatat ggatcaggtg atgaaccacc
    19141 tcttcgctac caccgatctg gaaaagagct atcgcattaa cctcaatatg atcggtctgg
    19201 atggtcgtcc ggcggtgaaa aacctgctgg aaatcctctc cgaatggctg gtgttccgtc
    19261 gcgataccgt gcgccgccga ctgaactatc gtctggagaa agtcctcaag cgcctgcata
    19321 tcctggaagg tttgctggtg gcgtttctca acatcgacga agtgattgag atcattcgta
    19381 acgaagatga accgaaaccg gcgctgatgt cgcggtttgg ccttacggaa acccaggcgg
    19441 aagcgatcct cgaactgaaa ctgcgtcatc ttgccaaact ggaagagatg aagattcgcg
    19501 gtgagcagag tgagctggaa aaagagcgcg accagttgca gggcattttg gcttccgagc
    19561 gtaaaatgaa taacctgctg aagaaagaac tgcaggcaga cgcgcaagcc tacggtgacg
    19621 atcgtcgttc gccgttgcag gaacgcgaag aagcgaaagc gatgagcgag cacgacatgc
    19681 tgccgtctga acctgtcacc attgtgctgt cgcagatggg ctgggtacgc agcgctaaag
    19741 gccatgatat cgacgcgccg ggcctgaatt ataaagcggg tgatagcttc aaagtggcgg
    19801 tgaaaggtaa gagcaaccaa tcggtagtgt ttgttgattc caccggtcgt agctatgcca
    19861 tcgacccgat tacgctgccg tcggcgcgtg gtcagggcga accactcacc ggcaaattaa
    19921 cgttgccgcc tggggcgacc gttgaccata tgctgatgga aagcgacgat cagaaactgc
    19981 tgatggcttc cgatgcgggt tacggtttcg tctgcacctt taacgatctg gtggcgcgta
    20041 accgtgcagg taaggctttg atcaccttac cggaaaatgc ccatgttatg ccgccggtgg
    20101 tgattgaaga tgcttccgat atgctgctgg caatcactca ggcaggccgt atgttgatgt
    20161 tcccggtaag cgatctgccg cagctgtcga agggcaaagg caacaagatt atcaacattc
    20221 catcggcaga agccgcgcgt ggagaagatg gtctggcgca attgtacgtt ctgccgccgc
    20281 aaagcacgct gaccattcat gttgggaaac gcaaaattaa actgcgcccg gaagagttac
    20341 agaaagtcac tggtgaacgt ggacgccgcg gtacgttgat gcgcggtttg cagcgtatcg
    20401 atcgtgttga gatcgactct cctcgccgtg ccagcaacgg tgatagcgaa gagtaatatg
    20461 ccggggaggg cctgtttcct cccgcttgca aggaacgccg gatgaaacgt taagtcgtca
    20521 tccggcagcg tattcaaccc ccggaaacga taaaaattcc cttgaaaccg ttgtttattc
    20581 atgcgttgcg attaacaata cgcttttcca gagagcggct tttaacaatg ccctaacctg
    20641 atttcaggtg acgtacaatg ccagtctctc agaccttcag agggtgttat gctatatatc
    20701 tttcgtctta ttattaccgt gatttacagc atcttagtct gtgtattcgg ctccatttac
    20761 tgccttttca gcccgcgtaa cccgaaacat gtggccacct ttgggcatat gtttggccgt
    20821 cttgcgccgc tgttcggcct gaaagttgag tgccgcaaac ctgcagatgc tgaaagctac
    20881 ggcaatgcta tctatatcgc taaccaccag aacaactatg acatggtgac agcatcgaac
    20941 attgtgcaac cgccgacggt gacggtaggt aaaaagagct tgctgtggat ccccttcttc
    21001 gggcagttgt actggttaac cggcaactta ttgatcgaca gaaacaatcg cactaaagct
    21061 cacggcacca ttgcggaagt cgtgaatcat ttcaaaaaac gccgtatttc catctggatg
    21121 ttcccggaag gaacccgcag ccgtggtcgc ggcctgctac cgttcaagac tggagcattt
    21181 cacgcggcaa ttgcggcggg cgtcccgatt attcccgtgt gcgtctctac aacttcgaat
    21241 aagattaatc ttaatcgact gcacaacggt ctggtgattg tcgaaatgct gccgccaatt
    21301 gacgtcagtc agtatggcaa agatcaggtt cgtgagctgg ctgcccattg tcgttcgata
    21361 atggaacaaa aaatcgccga gctcgataaa gaagtcgcag aacgcgaagc cgccggaaaa
    21421 gtttaagtcg gcaatctgta tttttgcggg gaacactttc ctgcacggta ttactttagc
    21481 cagttttaca tggagcaaat atgtcactca gtcggcgtca gttcattcag gcatcgggga
    21541 ttgcactttg tgcaggcgct gttcccctga aggccagcgc agccgggcaa cagcaaccgc
    21601 tacccgttcc gccgctactt gaatctcgcc gtgggcaacc gctgtttatg actgtacaac
    21661 gtgcgcactg gtcatttacg ccagggacac gcgcgtcggt ctggggaatc aatggtcgtt
    21721 acctggggcc gactatccgc gtctggaagg gcgacgatgt taagcttatt tacagcaacc
    21781 gcctgacaga aaatgtctca atgacggtgg ccgggctaca ggtacctggc ccgctgatgg
    21841 gcggtccggc acggatgatg tcgccaaacg ctgactgggc acccgtactg cctattcgcc
    21901 agaacgcagc tactctgtgg tatcacgcca atactcccaa ccgcacggct cagcaggtct
    21961 ataacggcct tgccggaatg tggctggtgg aagatgaagt cagcaagtcg ctgcctatcc
    22021 ccaaccatta tgatgtggat gattttccgg tcattatcca ggataaacgg ctggataact
    22081 ttggtacgcc agaatacaac gaaccgggaa gcggcggctt tgttggtgat acgctgctgg
    22141 ttaacggtgt acaaagcccg tacgttgaag tctcgcgtgg ctgggtgcgc ttgcgactgc
    22201 tgaacgcgtc gaactctcgt cgctatcaac tacagatgag cgatggtcgc ccgttacatg
    22261 tgatttctgg cgatcaggga ttcctgcctg ctcctgtatc ggtgaagcaa ctttcgctgg
    22321 caccgggcga gcgccgcgag attctggtgg atatgagcaa cggcgatgaa gtgtcgatca
    22381 cctgtggcga agcggcgagc attgttgatc gtattcgcgg cttctttgag ccatccagta
    22441 ttctggtttc taccctggtg ctaacgctgc gcccaaccgg ccttctgccg ctggtcacag
    22501 acagtcttcc gatgcgcttg ctgccaactg aaatcatggc cggttcgccg attcgcagtc
    22561 gcgatatcag tctgggtgat gacccgggta ttaatggaca gctgtgggac gtcaaccgta
    22621 ttgatgtcac cgcgcagcaa ggaacgtggg aacgctggac ggtacgcgcg gacgagccgc
    22681 aagcgttcca tattgaaggc gtgatgttcc agatccgtaa cgtgaatggt gcgatgccgt
    22741 tcccggaaga cagaggctgg aaagataccg tttgggttga cggacaagtg gagctgcttg
    22801 tttatttcgg tcagccttcc tgggcgcact tcccgttcta cttcaacagt cagacgctgg
    22861 aaatggcgga ccgtggctcg attgggcaac tgttagtcaa tccggtaccg taatcttcat
    22921 actcgcccga ggagggcgag tatggaatac ttttcgattt ctctttcgtt ccggcttata
    22981 atccccggcc tttgattatt tttttatcat ttctggaagc aaaatgagct ctatctccct
    23041 gatccaaccg gatcgcgacc tgttctcctg gccgcagtac tgggccgcct gttttggacc
    23101 ggcaccgttt ttgccgatgt ctcgtgaaga gatggatcaa cttggctggg atagctgcga
    23161 catcattttg gttactggcg acgcgtatgt cgatcaccca agcttcggga tggcgatttg
    23221 cggtcgtatg ctggaagcac agggctttcg cgtcgggatc atcgcccagc cggactggag
    23281 cagcaaagac gactttatgc gtctgggtaa accgaatctg tttttcggtg ttactgctgg
    23341 caacatggat tcgatgatca accattatac cgccgatcgc cgtttacgtc atgacgatgc
    23401 ctacacgccg gataacgtcg cgggtaagcg cccggatcgc gccacactgg tttataccca
    23461 gcgttgtaaa gaggcgtgga aagatgtacc ggtgatcctc ggcggtattg aggctagtct
    23521 gcgccgtacc gcacattatg attactggtc cgataccgtg cgccgttccg tgctggtgga
    23581 ttcgaaagcc gacatgctga tgtttggtaa cggtgagcgt ccgctggtgg aggtggcgca
    23641 ccgtctggcg atgggcgagc cgattagtga aatccgcgat gtgcgtaata ccgcgattat
    23701 cgtgaaagag gcgctgcctg gctggagcgg cgtggattcc ccccgtcttg atacccctgg
    23761 aaaaatcgac ccaatcccgc atccgtatgg tgaagatttg ccgtgcgcgg ataacaaacc
    23821 ggtggcaccg aaaaagcagg aagccaaagc cgtaaccgtg cagccaccgc gcccgaaacc
    23881 gtgggaaaaa acctacgtgt tgctgccttc tttcgagaaa gtgaagggcg ataaagtgct
    23941 gtacgcccat gcttcgcgta ttctgcacca cgaaaccaac ccaggctgcg tccgcgcatt
    24001 gatgcaaaaa cacggtgacc gctatgtgtg gatcaacccg cctgctatcc cgctttctac
    24061 cgaagagatg gacagcgttt ttgcgctgcc gtacaagcgc gtgccacatc cggcctatgg
    24121 caatgcccgt attccggctt acgaaatgat ccgtttttcg gtcaacatta tgcgcggctg
    24181 ctttggcggc tgttctttct gttctattac cgagcacgaa gggcgcatta ttcagagccg
    24241 ttcggaagat tcgatcatta atgagatcga agcgatccgc gacaccgttc caggttttac
    24301 gggcgtgatt tccgatctcg gtgggccaac tgccaacatg tatatgttgc actgcaaatc
    24361 gccacgcgct gaacaaactt gtcgccgttt gtcgtgcgtt tatccggata tttgtccgca
    24421 catggacact aaccacgaac cgacgatcaa cctctatcgc cgtgcgcgtg atctgaaagg
    24481 cattaaaaaa atcctcatcg cctcaggcgt acgttatgac atcgccgtgg aagatccgcg
    24541 ctatatcaaa gagttggcga cccatcacgt cggcggttat ctgaagattg ctccggaaca
    24601 taccgaagaa gggccgttgt cgaagatgat gaagccgggc atgggcagct atgaccgctt
    24661 taaagagctg ttcgatacct actcgaaaca ggcaggtaaa gagcagtatc tgatcccgta
    24721 tttcatctct gcgcaccccg gtacgcgtga tgaagatatg gtgaatttgg cgctgtggct
    24781 gaaaaagcat cgtttccgtc tcgaccaggt acagaacttc tacccatcgc cgctggcgaa
    24841 ctcaaccacc atgtattaca ccgggaaaaa accgctggcg aagattggtt ataagagcga
    24901 agacgtcttc gtaccgaagg gcgacaaaca gcgtcgtttg cataaagcgt tgttgcgtta
    24961 ccacgatccg gcaaactggc cgttaatccg cccggcgctg gaagcgatgg gcaaaaagca
    25021 tctgattggc agccgtcgcg attgcttagt gcctgcgcca accattgaag agatgcgtga
    25081 agctcgtcgc cagaaccgca atacccgtcc ggcgttgact aaacatacgc cgatggcgac
    25141 ccagcgtcag acgcctgcta cggcaaaaaa agcgtcgtct acgcaatctc gtccggtgaa
    25201 tgctggtgcg aagaaacgcc ctaaagcggc ggttggacgt taaaaaaaat ccccggcaac
    25261 tgacatgctg ccggggattt ttttatcatt ctgcgtacat acgcaagcag cgcaggcaat
    25321 caaaatagtc ctgagcatca agacgcgtat tggctctgtt agcttcggat ttggcagcat
    25381 agatttgtgg gcgtagtgct tttatatcct gctcaagccg ggcgattttg gcatttaatg
    25441 tttgtacatt gctcgccagt tgattgacct tttgctcatt gacgcagcca ctgagcaagc
    25501 aggttgccag aacaacagct cccacggtga aaatcgtttt catatagtgt tcccttttat
    25561 ttattgttcc ggctaattgt tatttaattc agctcattgc tttaatctgg cggcggtttt
    25621 tgcctcaatc tgcttaagta ggctgccgct gttcgggcgg ataaaatcag cgatttccca
    25681 cttgccttcg cgccgctgta aaaccagttc ttgctcttcg ttgttgccat tggtcagcgt
    25741 aaaacgaaca tgaatgcggg cggaatcggc ggttttctcc aggactcgaa tttcatcaac
    25801 gcgtgaaaat agcgactctt cgccaaaaca gatcggattg tcgtaaataa attgccgttc
    25861 tatttgcata tcttcatcgg gattattggc cgtcttgaag g
//
LOCUS       DAJVQG010000046        25645 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJVQG010000046 DAJVQG010000000
VERSION     DAJVQG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25645
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.046"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..394
                     /locus_tag="OQM83_001710"
     CDS             <1..394
                     /locus_tag="OQM83_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCS1519992.1"
                     /translation="WDFGLIVLRNPPSGITPLPLFEGDKAALTAALKSAGRKVTQAGY
                     PEDHLDTLYSHQNCEVTGWAQTSVMSHQCDTLPGDSGSPLMLHTNDGWQLIGVQSSAP
                     AAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(433..762)
                     /gene="mdtI"
                     /locus_tag="OQM83_001711"
     CDS             complement(433..762)
                     /gene="mdtI"
                     /locus_tag="OQM83_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCS1519993.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(749..1114)
                     /gene="mdtJ"
                     /locus_tag="OQM83_001712"
     CDS             complement(749..1114)
                     /gene="mdtJ"
                     /locus_tag="OQM83_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCS1519994.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            1526..2560
                     /gene="tqsA"
                     /locus_tag="OQM83_001713"
     CDS             1526..2560
                     /gene="tqsA"
                     /locus_tag="OQM83_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCS1519995.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLISVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(2585..3973)
                     /gene="pntB"
                     /locus_tag="OQM83_001714"
     CDS             complement(2585..3973)
                     /gene="pntB"
                     /locus_tag="OQM83_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCS1519996.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(3984..5516)
                     /gene="pntA"
                     /locus_tag="OQM83_001715"
     CDS             complement(3984..5516)
                     /gene="pntA"
                     /locus_tag="OQM83_001715"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCS1519997.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            6040..6984
                     /locus_tag="OQM83_001716"
     CDS             6040..6984
                     /locus_tag="OQM83_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1519998.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            7170..8552
                     /locus_tag="OQM83_001717"
     CDS             7170..8552
                     /locus_tag="OQM83_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1519999.1"
                     /translation="MKKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            8589..9311
                     /gene="folM"
                     /locus_tag="OQM83_001718"
     CDS             8589..9311
                     /gene="folM"
                     /locus_tag="OQM83_001718"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCS1520000.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(9308..9643)
                     /locus_tag="OQM83_001719"
     CDS             complement(9308..9643)
                     /locus_tag="OQM83_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCS1520001.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            9772..10491
                     /gene="rstA"
                     /locus_tag="OQM83_001720"
     CDS             9772..10491
                     /gene="rstA"
                     /locus_tag="OQM83_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCS1520002.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIVPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            10495..11796
                     /gene="rstB"
                     /locus_tag="OQM83_001721"
     CDS             10495..11796
                     /gene="rstB"
                     /locus_tag="OQM83_001721"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCS1520003.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            11872..12801
                     /gene="tus"
                     /locus_tag="OQM83_001722"
     CDS             11872..12801
                     /gene="tus"
                     /locus_tag="OQM83_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCS1520004.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12798..14201)
                     /gene="fumC"
                     /locus_tag="OQM83_001723"
     CDS             complement(12798..14201)
                     /gene="fumC"
                     /locus_tag="OQM83_001723"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCS1520005.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(14344..15990)
                     /gene="fumB"
                     /locus_tag="OQM83_001724"
     CDS             complement(14344..15990)
                     /gene="fumB"
                     /locus_tag="OQM83_001724"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCS1520006.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            16189..17364
                     /gene="manA"
                     /locus_tag="OQM83_001725"
     CDS             16189..17364
                     /gene="manA"
                     /locus_tag="OQM83_001725"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCS1520007.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            17465..18973
                     /locus_tag="OQM83_001726"
     CDS             17465..18973
                     /locus_tag="OQM83_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCS1520008.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(19154..20464)
                     /gene="uidC"
                     /locus_tag="OQM83_001727"
     CDS             complement(19154..20464)
                     /gene="uidC"
                     /locus_tag="OQM83_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCS1520009.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(20503..21876)
                     /gene="uidB"
                     /locus_tag="OQM83_001728"
     CDS             complement(20503..21876)
                     /gene="uidB"
                     /locus_tag="OQM83_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCS1520010.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGFCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(21873..23684)
                     /gene="uidA"
                     /locus_tag="OQM83_001729"
     CDS             complement(21873..23684)
                     /gene="uidA"
                     /locus_tag="OQM83_001729"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCS1520011.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(24073..24663)
                     /gene="uidR"
                     /locus_tag="OQM83_001730"
     CDS             complement(24073..24663)
                     /gene="uidR"
                     /locus_tag="OQM83_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCS1520012.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(24884..25630)
                     /gene="hdhA"
                     /locus_tag="OQM83_001731"
     CDS             complement(24884..25630)
                     /gene="hdhA"
                     /locus_tag="OQM83_001731"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCS1520013.1"
                     /translation="MRHAPCAIITGAGAGIGKEIAITFATAGASVVVSDINADAANHV
                     VDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPKPFDMPMA
                     DFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYASSKAAAS
                     HLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRLGQPQDIA
                     NAALFLCSPAASWVSGQILTVSGGGVQELN"
BASE COUNT         6420 a         6660 c         6147 g         6418 t
ORIGIN      
        1 gtgggacttc ggattgattg tgctacgtaa tcccccttct ggcattacac cgttgccgtt
       61 atttgaggga gataaagccg cgcttactgc cgcattaaaa tcggctggcc gtaaagtaac
      121 tcaggcggga taccctgaag atcatctcga tacgttgtac agtcatcaaa actgtgaagt
      181 gactggctgg gcgcaaacgt cggtgatgtc acatcagtgc gataccttgc cgggtgacag
      241 cggttcgcct ctgatgttgc ataccaatga cggctggcaa ttaattgggg tgcaaagttc
      301 ggctcctgcc gcgaaagatc gctggcgcgc cgataaccgg gccatttctg ttaccggttt
      361 tcgcgacaag ctggatcaac tgtcgcaaaa ataatgttca aacgctgccc gactgtgcgg
      421 gcagcgtgtt catcaggcaa gtttcaccat gatcattcca gccaacagca agaccaggcc
      481 aatccagcct ttacgattta accgttgacc aaacaagatc caacctgcgg ctaacgtggc
      541 ggcaatacca aacccgcccc acaatgcata agcgacagac aagtcgatcc ctttaacggc
      601 ctgagaaagc gcactaaagg cagccagcac cgccgccagg gagagcaagc caaatatttt
      661 gcgacgaaag ccgtcagaaa atttcaaaaa gacgttagca acgatttcca gcacgattgc
      721 caatgccagc caggcggcgt gaacccattc aaactgcgcc atggttcacc tccagttcag
      781 gtttacgcgc tttacgggta cctgatttta tcaacacaat cccggcgacc agggtggtta
      841 acccggcaat tttcatcagc gataaacttt cgtcgaataa caaaacgcta aacaaggtaa
      901 taaataaaat accgatacct tcccacagcg cataagctac gcctaaggcg atctttttaa
      961 cggcgaaaga gagaaatata tacgacagag aaatcatcac cagcattaaa ataaagccgc
     1021 catttccctc actgacgctc gcccatttca ttgacagcgt accggtaatt tctgtagcaa
     1081 tagccaggcc taataaaatc caataaatat acattgtcct tctcctgcaa gagaattatt
     1141 ttaattttcg cttaattcag cgaaaccaaa gtaaagtggt caagccccgc gcatagggca
     1201 agcttaggca gaagaaagga ctaaagcgcg ttgcgccagt gctgctcacc tacgagcaag
     1261 atagtggatg aggtacgaag atgggtaaat gttgaaaata acgtcctgaa caaattgtcc
     1321 ataatattac aattatccgc agtgttgctt ctcgtcatcg cggatgataa ttgtcctcgg
     1381 tagttgaaca cgcctgattt gtatcatagc ttaagaatta actcaaaata ttttcacttc
     1441 tttacctgag cggtttgatt ttcgttatga tgacggagcg aaaaagacat tattattagc
     1501 aaaggaagaa aaaacgggga caagcatggc aaagccgatc atcacgctca atggcctaaa
     1561 aatcgtcatt atgttgggaa tgctggtcat tattctctgc ggtatccgtt ttgccgccga
     1621 gatcatcgtg ccgtttattc tcgcattatt tattgctgtt attcttaacc cgctggtgca
     1681 acacatggtc cgctggcgag tgccgcgtgt actggcggtg tcgattttga tgaccatcat
     1741 cgtgatggcg atggtgttgc tattagctta tctgggttcc gcgctcaacg agttgacgcg
     1801 gacgttaccg caatatcgca actctattat gacgccgctg caagctcttg agccgttgtt
     1861 gcaacgcgta gggattgacg tctcagttga ccagctggcg cattacattg atccgaacgc
     1921 ggcgatgacg ttgctcacca acttattgac gcagttatct aatgccatgt catcaatatt
     1981 tttattgctg ctgacggtgc tgtttatgct gctcgaagtg ccacaattgc ccggaaaatt
     2041 tcagcaaatg atggcgcgtc cggttgaagg gatggcggcg attcaacgtg caattgacag
     2101 tgtttctcat tatctggtgc tgaaaacagc catcagcatc atcaccggcc tggtcgcctg
     2161 ggcgatgctc gccgcactcg atgttcgctt cgcttttgtc tggggattgc tggcctttgc
     2221 gcttaattac atccctaata ttggttcagt tctcgcggca atccccccta tcgctcaggt
     2281 actggtgttt aatggcttct acgaagcgtt gctggtgctg gcgggatatc tgctgattaa
     2341 tctggtcttc ggcaatattc tggagccgcg catcatggga cgtgggctgg ggctttccac
     2401 attggtggta tttttgtcgt tgattttttg gggatggttg ttaggaccgg tgggtatgct
     2461 gatttccgtg ccgttaacaa ttattgtcaa aattgcgctt gaacaaacag cgggaggtca
     2521 aagcatcgcc gttctgttaa gcgatctcaa taaagagtga cggcctccgc agaggccgtc
     2581 agggttacag agctttcagg attgcatcca cgctagcttt ggcgtcacca aacagcatgt
     2641 gggtgttttc cttgaagaac agcgggtttt gcacaccagc atagccagtg ttcatcgaac
     2701 gtttaaagac aatcacgttc tgcgctttcc acacttccag cacaggcata ccagcaatag
     2761 gactcttcgg atcatcctgc gccgccgggt taaccgtatc gttagcacca atcaccagta
     2821 cggtatcggt atcagcaaag tcatcgttga tctcgtccat ttccagcacg atgtcatacg
     2881 gtacttttgc ttcagccagc aatacgttca tatgtccagg caaacgcccc gcaaccgggt
     2941 ggataccgaa acgcacgttg ataccacgag cgcgcagttt ctcggtaatt tcagcgacag
     3001 gatattgcgc ctgcgcgact gccatgccgt accccggagt aatgatcact gaatgggagt
     3061 ttttcagcag ttccgctgtc tcttctgcgg tgatttcgcg gtgctcgccc acttcctgat
     3121 catcgccagt agaagagccg tcggtgccga aaccacccgc aataacgctg ataaaggaac
     3181 ggttcatcgc cttacacata atgtaagaaa ggatagcccc cgaagaaccg accagcgcac
     3241 cggtcacaat cagcaggtcg ttgctgagca taaagcccgc agccgcagcc gcccagccgg
     3301 agtacgagtt cagcatcgac accaccactg gcatatctgc accaccgatg gaggcgacta
     3361 aatgccagcc gaataccagc gcaatggcgg tcattattag caatgccagc acttgcaggc
     3421 cgacgctgtc cgtgcgaaca aatacaatca gcagcaggaa ggaaacgacc agagccgcca
     3481 ggttcatttt gtgacggttt ggcagcatca acggtttaga cgaaatcttg ccacacagtt
     3541 tgccgaacgc caccaccgaa cccgtgaacg ttaccgcccc gatgaagata ccgaggaaca
     3601 cttccgtcag gtgaatattg accagaatcg gtgccattcc cgcgtcatga tgcagatagc
     3661 tgttaaagcc aaccagcact gccgccagac ccacgaagct gtgcaggatc gccaccagtt
     3721 ctggcatttc ggtcatttca actttcttcg ccagacggat accaattgcc ccaccaatga
     3781 ccatcgccag caagatccag ccaacattac ccgtatccgg tccaaaaatg gttgcgatta
     3841 acgcaatcgc catcccggcg ataccgaagt tgttaccctg gcgagacgtt tcatgtttcg
     3901 aaagaccggc cagactgaag ataaacagga tcgcggcaac aatgtatgca gctgtaacta
     3961 atcctccaga catatgttac cccttaattt ttgcggaaca ttttcagcat gcgctgagtc
     4021 acggtgaagc caccgaaaat attaatgctg gctataagca ccgcgataaa actaaggaag
     4081 ctaacccagc cgccctggcc aatctgcaac agtgctccga caacaataat ccctgaaatc
     4141 gcgttggtga ccgacatcaa cggtgtatgc agcgcgtgcg atacattcca caccacgtaa
     4201 taaccgacaa cgcaggccag cgcgaaaacg gtgaagtgcc caagaaactc tttcggcgca
     4261 acgcttgcca gccaaccgaa aaggatgatt gccagcgcca tcaacgcgta tttacgccac
     4321 ggtgagcagg cacatttttc ctcagttttc acttccggtg ccgctttttg tgccgcctgc
     4381 ggctgagctg atacctgaat cggcggtgcc ggccaggtaa tttcgcccgc acggatcacg
     4441 gtcacgccgc gaatcaccac atcatcaaaa tcaacagtga tattgccgtc tttctctttg
     4501 cacaacagtt tcagcagatt aacgaggtta gtaccgtaaa gctgcgagga ttgcgtcggc
     4561 agacggcccg gaagatcggt ataaccaatc actttgacac cattttccgt agtgaagatt
     4621 tcacccggca cggtgtattc acagttgcca ccgttttggg ctgccaggtc gacaatcaca
     4681 ctgcccgcct tcatggagtc aaccatttca cgggtaatta gcttcggcgc tggtttgcct
     4741 ggaataagcg cggtggtgac aatgatatcg acctcttttg cctgggcggc aaagagttcc
     4801 atttccgctt taatgaacgc gtccgacatc actttggcat agccatcgcc gctgcccgct
     4861 tcctctttaa aatccagctc gaggaattcc gcgcccatac tttgaacttg ttctttcact
     4921 tccggacggg tgtcgaatgc acgcacaatc gcgccgagac tgtttgctgc gccaatggcg
     4981 gccagacctg caacacccgc accaatcacc atcacttttg ccggtggcac tttcccggcc
     5041 gcagtaattt gcccggtaaa gaagcgccca aattcatgtg ccgcttcaac aatggcgcga
     5101 taaccggcga tgttcgccat cgagcttagt gcgtccagcg attgtgcgcg tgagatacgc
     5161 ggcacagaat ccatcgccat cacggtcacg ttacgttccg caagtttttg cattaattcc
     5221 ggattctgcg caggccagat aaaactcacc agcgttgtcc caggattcag taacgcaatt
     5281 tcatcatcta acggcgcatt gaccttcaga atgatctctg actgccagac gctattccct
     5341 tctacaattt cagcgcccgc ttgcacaaac gctttatcgt caaaacttgc cagttgaccc
     5401 gcgccgctct ctaccgcgac ggtaaaaccc agtttcagca gctgttccac tgtttttggc
     5461 gttgctgcaa cacgggtttc attggttaac cgttctcttg gtatgccaat tcgcatgata
     5521 ttcccttcca tcggttttat tgatgatggt ttgcctgtgt caggagccac acaagccgct
     5581 catgtacgag ctaaatgtta ctccgttaaa ataaattagt aacaaacgcc ctataacgta
     5641 ctgaaaatta tgcctgtgat ctagcgccaa aaatcagtat ttcggcgtga actcgcaaaa
     5701 tattaacgat tcagccgtga tagtgggata aacaccttag aacgccggat aaagactgat
     5761 aattgtcttc gacggccggg taaaacgaga catcgccccg gcacgattca ctacttaaca
     5821 ttaaattaac ttatacaatt cagttgcttc agtagtaatg atgctgatac ggctgttttt
     5881 taagcataga cggtcatttg agcaggatta aaattggctt aaggaatgtg atatgaaaaa
     5941 tgacgcagac agttacaccg tttaaatgca ataatcagcc acgtttctcg ttaataacaa
     6001 taccagtacc tggtttgcgc aaggcgaagg attattttta tgaagcttaa gaacaccctc
     6061 ctggcgtcgg cactgctttc tgctacggca ttctccgtta acgcagcaac agaactgaca
     6121 ccggagcaag cggcagcggt taaacctttt gaccgtgtag tggttaccgg tcgttttaat
     6181 gctattggcg aagcggtgaa agccgtttct cgtcgcgcag ataaagaagg tgccgcctct
     6241 ttttatgttg tcgacacttc tgattttggt aacagcggta actggcgtgt ggtcgctgac
     6301 ctctataaag ccgatgctga aaaagcagaa gaaacaagta atcgcgtaat taacggtgtt
     6361 gtcgaactgc cgaaagatca ggctgttctg attgaaccgt ttgacacggt caccgtccag
     6421 ggcttctatc gtagccagcc agaagtcaat gatgccatca ccaaagcggc aaaagcgaaa
     6481 ggtgcctact ctttctacat cgttcgtcaa atcgatgcca accaaggcgg caaccagcgt
     6541 attactgcat tcatctataa aaaagatgct aagaaacgta tcgtccagag cccggatgtg
     6601 atcccggcag attccgaagc aggacgtgca gctctggctg ccggtggcga agccgcgaag
     6661 aaagttgaga tcctaggtgt tgcgactacc gcatcaccaa gttctgaagt cggtcgtttc
     6721 tttgaaaccc agtcatcaaa aggcgggcgt tacaccgtca tgctcccgga tggcactaaa
     6781 gtcgaagaac tgaacaaagc gaccgcagcg atgatggtcc cgttcgacag catcaaattc
     6841 tctggcaact acggcaacat gaccgaagtc tcctatcagg ttgcgaaacg tgccgcgaag
     6901 aaaggtgcta agtattacca catcacccgc cagtggcagg aacgtggtaa taacctgacc
     6961 gtcagcgcag atctgtataa ataactattc agttactcct ataaggcggc ttgatgccgc
     7021 ctttcgcatt tttagtcaca aatttccccg cgaccattgc atgcagacac atctcttcgt
     7081 aaaatcccgc gcttcagtgg caaactccat ttttatgcgt tttgccctaa tatttattct
     7141 tcttatcacg ttttaatcac tggatatcga tgaaaaagaa actgggactg agcgcactca
     7201 ccgcgctggt attaagctca atgctgggcg cgggtgtttt cagtctgccg caaaatatgg
     7261 cggcagttgc cagcccggca gcactgctca tcggctgggg tattactggc gctggcattt
     7321 tattgctggc ctttgccatg ctgatcctca cgcgcattcg tcccgaactt gacggcggta
     7381 tctttaccta tgcccgcgaa gggtttggcg agctaatcgg cttttgttcc gcatggggat
     7441 actggctgtg cgcagtcatc gccaacgtct cctatctggt gatcgttttt tccgcgttaa
     7501 gcttttttac ggacacgccg gaattgcgcc tgttcggcga tggtaatacc tggcaatcga
     7561 tcgtcggtgc atcggcttta ttgtggattg ttcacttttt gatcctgcgc ggggtgcaaa
     7621 ccgctgccag cattaacctg gtggcgacac tggcaaaatt gttgccgctg ggtctgtttg
     7681 ttgtgctggc gatgatgatg ttcaaactgg ataccttcaa gctcgacttc accggacttg
     7741 cacttggcgt acccgtttgg gaacaggtga aaaacaccat gctgatcacc ctgtgggttt
     7801 tcattggtgt ggaaggtgcc gtggttgttt ctgcgcgtgc gcgtaataaa cgtgatgttg
     7861 gcaaagcgac actgctggcg gttctctccg ctctgggcgt ttacttgctg gtaacgctgc
     7921 tttcgctggg tgtggtggca cgtcctgaac tggctgaaat tcgtaacccg tcaatggccg
     7981 gtctgatggt ggaaatgatg ggaccatggg gcgaaatcat catcgctgcc ggtttgatcg
     8041 tttccgtttg cggcgcgtac ttaagctgga ccatcatggc ggcagaagtt ccgttcctgg
     8101 ctgccactca taaagcattc ccacgcattt ttgcgcgcca gaacgcacaa gctgcgccat
     8161 ccgcctcact gtggttgacc aacatctgcg tgcaaatttg tctggtactc atctggctga
     8221 ccggttccga ttacaacacg ttgctgacca tcgcctcaga aatgattctg gtgccctatt
     8281 tcctggtcgg cgcgttcttg ctgaaaatcg caacccgtcc actgcataaa gcggtaggtg
     8341 tcggtgcctg catttatggc ttatggttat tgtacgcctc tggtccaatg cacttgctgt
     8401 tatccgttgt tctgtatgcg ccggggctgc tggttttcct gtatgcgcgc aaaacgcata
     8461 cacatgataa tgtactgaac cgtcaggaaa tggtgctgat tggcatgctg ttaattgcct
     8521 cagttccggc gacctggatg ctggtgggat aacgttcatc ccatcgttgg atagcaaagg
     8581 agataacgat gggtaaagcc cagcccttgc caatattaat tactggcgga ggtcgtcgca
     8641 tcggcctcgc cctcgcctgg catttcatta atcaaaagca accggtgatt gtcagctatc
     8701 ggacgcacta tccagccatt gatggactga ttaaggcagg tgcgcagtgt attcaggctg
     8761 atttttcgac caacgacggt gtgatggcgt ttgccgatga agtactaaaa agcccccatg
     8821 gtctgcgtgc tattttgcat aacgccagtg cgtggatggc ggaaaaaccg ggtgcgccac
     8881 tggccgacgt actggcttgc atgatgcaga tccacgttaa taccccatac ctgctcaacc
     8941 atgcgctgga aagattactg cgtgggcacg gacacgccgc cagcgatatc attcacttta
     9001 ccgattatgt ggtggagcgc ggtagcgaca agcatattgc gtatgctgca agcaaagcgg
     9061 cactggataa tatgacccgc tcgtttgccc gcaagctggc accggaagtg aaagtgaact
     9121 ctattgctcc atcgctgatc ctgtttaatg aacatgatga tgccgaatat cgacaacagg
     9181 cgctgaataa atcactgatg aaaaccgcgc ctggcgagaa agaagtgatc gacctggtcg
     9241 attacttact taccagttgc tttgtcaccg gacgcagttt cccacttgat ggcggtcgtc
     9301 atctgcgtta atgcagcttt atccagcata taatcaacac ccatgcgctt atcccccaac
     9361 acgaaacaga accaacaaaa gcggcgggca agcgcatcat tccggtgaaa taccacagcg
     9421 acaccaggta aacaaaatag ggaataatcg accacatact aaaaatgatg gttgcgcgta
     9481 aggcttcaat gccgcgttcg ctggcaacaa tataatgcgc gataagcgca aaggtcggga
     9541 aaagtggaat cagcccggcg atataataat ttttcgtttt tgctaaaaca ccaatcaaca
     9601 gcactaccag cgcaccgagc gcggctttga ttaccagccc cattttttta ccttaacact
     9661 tccataacaa gtcatcagta gaatacctga tgaaaacttg tttagaaacg attgatagta
     9721 agtaaaaaca gcgcggtgta ttgtgacgtt tttatatcta ccgtgaatgt tatgaacact
     9781 atcgtatttg tggaagatga tgcggaagtc ggttcactga ttgccgcgta cctggcaaaa
     9841 catgatatgc aggttaccgt agagccgcgc ggcgaccagg ccgaagaaac cattttgcga
     9901 gaaaatccgg atttggtgtt actcgacatc atgctaccag gcaaggacgg catgaccatt
     9961 tgtcgtgatt tacgcgcaaa gtggtctgga ccgattgttc ttctaacctc tctcgatagc
    10021 gatatgaacc acatcctggc actggaaatg ggtgcctgcg actatattct caaaacgacg
    10081 ccccctgctg ttttgctagc gcgtttacgt ttgcatttgc gtcagaatga gcaagccaca
    10141 ctgaccaaag gtcttcagga aacgtctctg actccctaca aagccctgca tttcggcacg
    10201 ttgaccatcg ttcccatcaa ccgcgtagtc accctggcta acactgaaat ctcgctctcg
    10261 acagctgatt tcgaattatt gtgggaatta gctacccatg ccgggcaaat catggaccgc
    10321 gatgcattgc tgaaaaattt acgcggcgtc agttatgacg gactggatcg tagcgtggac
    10381 gtggctattt cgcggttaag aaaaaaactg ctcgataacg ccgcagaacc ttatcgcatt
    10441 aaaactgtgc gtaacaaagg ctatcttttt gcgcctcatg catgggaata agcgatgaaa
    10501 aaactgttta tccagtttta cctgttattg tttgtctgct tccttgtgat gtctctgctg
    10561 gttgggctgg tgtacaaatt taccgccgaa cgcgcgggca aacagtcgct ggatgatttg
    10621 atgaacagtt cactgtatct gatgcgcagc gaattgcgtg agatcccccc acacgactgg
    10681 ggtaagacgc tgaaagagat ggatttaaat ctctctttcg atctgcgtgt cgagccactg
    10741 agtaaatacc atcttgatga tatttccatg caccgactgc gtggcggcga aattgtcgcc
    10801 ctggacgatc agtacacgtt tttgcagcgt atcccgcgca gccactacgt gctggcagtt
    10861 ggtcctgttc cttatcttta ttatctccat cagatgcgat tgctggatat cgccctgatc
    10921 acttttattg ctatttccct cgcctttccg gtgtttatct ggatgcgtcc gcactggcag
    10981 gatatgttaa aactggaagc agcggcgcaa cgatttggcg atgggcatct caatgaacgt
    11041 atccactttg atgagggttc gagctttgaa cgacttggta tcgcatttaa ccagatggcg
    11101 gacaatatca acgccttgat tgccagcaaa aaacagctta ttgacggtat cgctcacgaa
    11161 ctgcgaacac cgttagtgcg cctgcgttat cgactggaga tgagcgataa cctgagcgcc
    11221 gccgaatccc aggcgttgaa tcgtgatatc agtcaacttg aagctttaat tgaagagctg
    11281 ctgacttatg cccgactcga tcgcccacaa aacgagcttc atcttagcga accagacctg
    11341 ccgttgtggc tgtcaacgca tctggcagac attcaggcag taacgcccga taaaacggta
    11401 cggattaaaa cgctcgcgca aggccattat gcggcgttgg atatgcgctt aatggagcgc
    11461 gtgctggata atttgctcaa taacgccctg cggtactgcc attcaacggt tgaaaccagc
    11521 ctgctactgt cggggaatag agcgacatta attgttgagg atgatggccc agggattgcc
    11581 ccagaaaacc gcgaacatat ctttgaacct tttgttcgcc tcgatcccag ccgggatcgc
    11641 tcaaccggcg gctgcgggct ggggctggca attgtccact ctatagcact ggcaatgggc
    11701 ggtacggtta attgtgacac cagcgaactg ggtggtgctc gcttctcgtt tagctggccg
    11761 ttatggcata acatcccgca atttacctct gcctgacact acgcgcacga tggtcaagtc
    11821 accaccactg tgctataaaa taagtatgtt gtaactaaag tggttaatat tatggcgcgt
    11881 tacgatctcg tagaccgact caacactacc tttcgccaga tggaacaaga gctggctgca
    11941 tttgccgctc atcttgagca acacaagcta ttggttgccc gcgtgttctc tttgccggag
    12001 gtaaaaaaag aggatgagca taatccgctt aatcgtattg aggtaaaaca acatctcggc
    12061 aacgacgcgc agtcgctggc gttgcgtcat ttccgccatt tatttattca acaacagtcc
    12121 gaaaatcgca gcagcaaggc cgctgtccgt ctgcctggcg tgttgtgtta ccaggtcgat
    12181 aacctttcgc aagcagcgtt ggtcagtcat attcagcaca tcaataaact caagaccacg
    12241 ttcgagcata tcgtcacggt tgaatcagaa ctccccaccg cggcacgttt tgaatgggtg
    12301 catcgtcatt tgccggggct gatcaccctt aatgcttacc gcacgctcac cgttctgcac
    12361 gaccccgcca ctttacgctt tggttgggct aataaacata tcattaagaa tttgcatcgt
    12421 gatgaagtcc tggcacagct ggaaaaaagc ctgaaatcac cacgcagtgt cgcaccgtgg
    12481 acgcgcgagg agtggcaaag aaaactggag cgagagtatc aggatatcgc tgccctgcca
    12541 cagaacgcga agttaaaaat caaacgtccg gtgaaggtgc agccgattgc ccgcgtctgg
    12601 tacaaaggag atcaaaaaca agtccaacac gcctgcccta caccgctgat tgcactgatt
    12661 aatcgggata atggcgcggg cgtgccggac gttggtgagt tgttaaatta cgatgccgac
    12721 aatgtgcagc accgttataa accacaggcg cagccgcttc gtttgatcat tccacggctg
    12781 cacctgtatg ttgcggatta acgcccggct ttcatactgc cgaccatctg ttctggccgt
    12841 acccagctgt caaactcggc ttcgctaaga taccccagcg caagggccgc agcttttaag
    12901 gtcagccctt ctttatgcgc ttttttggcg atctccgcgg ctttgtcata accaatgtgg
    12961 gtgttaagcg cagtcaccag catcagcgat tcattgagta attgattgat tcgctcacga
    13021 ttcggttcaa tacccactgc gcagtgtttg ttaaaacttt ccatgccatc tgccagcaag
    13081 cgcaccgatt gcaggaaatt gtggatcacc attggacgga agacgttcag ttcaaagtta
    13141 ccggaagcgc cacccatgtt gatcgccacg tcgttcccca ttacctgaca gcagagcatg
    13201 gttaatgctt cacactgggt tgggttcact ttccccggca tgattgagct gcccggctca
    13261 ttttccggga ttgagatttc accaattccg cagcgcgggc cagaggccag ccagcggaca
    13321 tcattggcga ttttcatcag tgacgcagcc aaccctttca acgcgccgtg cgcctgaacc
    13381 agggcatcac aggtcgcaag cgcttcaaat ttgttcggcg cggtaacaaa cggtgcacag
    13441 gtaatgactg ccagttcatc tgctacgcga cgcgcatact ccggatgggt atttagtcca
    13501 gtacccaccg ctgtaccgcc gagagccagt tccgctacgt gaggcaggct gtattcgata
    13561 tgtttgagat tatgctcgag catcgctacc cagccggaaa tctcctgccc cagtgttagc
    13621 ggcgtggcgt cctgcaagtg ggttcgaccg attttgacga tatcggcaaa tgcacgcgat
    13681 ttttcactca gtgtctgtgt cagggtttta acctgcggaa tgagttgctt gcgcagcgcc
    13741 agcagcgccg caacgtgcat cgccgtcgga aagacatcgt tggaactttg gcttttgttc
    13801 acgtcgtcgt tagggtgaac tttacgttcc atcccgcgca cgccgccgag taattcactg
    13861 gcccggttag ccagcacttc gttcatgttc atgttacttt gcgtgccgga gccggtctgc
    13921 cagatagcca gcgggaattc gtcgtcatgc tgtcctgcca gtacttcatc cgccgcctgc
    13981 cgaatggcgc tcgctttctc ttcagacaac aagcctaaat cttcattaac ttttgccgct
    14041 gcgcgcttgg ttagcgccag cgcatgaatc agtgaggtgg gcattttctc cgtcgaaatg
    14101 cggaaatgct ccagcgagcg ttgagtttgt gcgccccaca gcttatctgc cgggacatca
    14161 atcgccccca tcgaatcttt ttcgctgcgt actgtattca tggcctgctc ctcacctgat
    14221 taattttttc tttctgtttt gccttcgtta agcaactttt agctcactta ttatttacta
    14281 tttgataaca aatgtttggt ctttcgtgcc atgtaaaaaa accgccccga agggcggctc
    14341 tgtttatttc acacagcgag tgcactgtgt gagttgtatc tgctggaaga agtcatttcc
    14401 tttatcatcc acaaggataa acgccgggaa atcttccact tcaattttcc agatggcttc
    14461 cattcccagt tccggatatt caacacattc caggctctta atacttccct gcgccaatac
    14521 agcggccgga ccaccgatac tgccaaggta gaagccgccg tgttttttac aggcatccgt
    14581 cacctgctgg ctgcggttgc ctttcgccag catgatcata cttccgccct gcgcttgcag
    14641 ttgatcgaca taagaatcca tccgtccggc ggtcgttggg ccaagagaac cggaggcata
    14701 accttccggc gttttggccg gacccgcgta gtaaatcgga tgatctttaa tgtactgcgg
    14761 cagcccttca ccgttatcca tccgctcttt cagtttggcg tgagcaatat cacgaccgac
    14821 gataatcgtg ccgttaagcg ataagcgtgt agaaacggga tactgcgaca actgtgcgag
    14881 gatctctttc atcggacggt taaggtcaac gcgcaccgct tcgccttctc ccgctttacg
    14941 cagctcttca gggatatatt tacctggatt atgttccagt ttttcgatcc agatcccctg
    15001 acggttgatc ttcgctttga tattacggtc agcagagcag gaaacaccca taccaaccgg
    15061 acaggatgcg ccgtgacgtg gcaggcgaat cacgcgtatg tcgtgagcga agtatttacc
    15121 accaaactgc gcacccagac cgagattttg cgcttcgatc agcaattctt tttccagttc
    15181 cacatcgcgg aatgcctgac cgtgctcatt cccttccgtt ggcagttcgt catagtattt
    15241 agcggaagcc agtttcactg ttttaaggtt agtttctgca gaagttccac caattacgaa
    15301 cgcaatatga tacggaggac aggccgccgt acccagcgtg cgcatcttct caaccaggta
    15361 atttttcagt ttccccggcg ttagtaacgc tttggtttcc tgatagagat acgtcttgtt
    15421 tgccgaacca ccacctttgg cgatacagag gaatttgtac tcgtcgccat caacggcata
    15481 aagatcgatc tgcgctggca gattggtgcc ggtattcacc tctttataca tatccagcgg
    15541 cgcgttttgc gagtagcgca aattatcttc gatataagtg ttatagacac cgcgcgccag
    15601 cgccgcttca tcacagccac cggtccatac acgctgccct tttttaccaa caataatcgc
    15661 agtgccggta tcctgacagg ttggcagaac acctttcgcc gcgatgtcgg agttgcgcag
    15721 gaattgcagc gccacatatt tatcattttc gctggcttcc gggtcacgca gaatgtcggc
    15781 cacctgttgt tggtgagccg gacgcagcat aaatgacgca tcatgaaacg cctgacgcgc
    15841 caacagagtt aacgcttcgg gtgcgacttt caaaatctcc tgcccttcaa attcagatac
    15901 gctaacgtgt tcgctggtta gcaggtaata ctcagtatca tcttttttga gtggaaaagg
    15961 agcctgataa tgaaagggtt tgtttgacat tgttctctca cttactgcct ggtttggtta
    16021 tgctctgggc gggtgttccg ttgccctgtt aaaagcgagt aacaatatcc tacacacttt
    16081 tttaacaaaa actgagacta gtacgacttt ttgcggctcc aggttacttc ccgtaggatt
    16141 cttgctttaa tagtgggatt aatttccaca ttaaaacagg gattgatcat gcaaaaactc
    16201 attaactcag tgcaaaacta tgcctggggc agcaaaacgg cgttgactga actttatggt
    16261 atggaaaatc cgtccagcca gccgatggcc gagctgtgga tgggcgcaca tccgaaaagc
    16321 agttcacgag tgcagaatgc cgccggagat atcgtttcac tgcgtgatgt gattgagagt
    16381 gataaatcga ctctgctcgg agaggccgtt gccaaacgct ttggcgaact gcctttcctg
    16441 ttcaaagtat tatgcgcagc acagccgctc tccattcagg ttcatccaaa caaacgcaat
    16501 tctgaaatcg gttttgccaa agaaaatgcc gcaggtatcc cgatggatgc cgccgagcgt
    16561 aactataaag atcctaacca caagccggag ctggtttttg cgctgacgcc tttccttgcg
    16621 atgaacgcgt ttcgtgagtt ttccgagatt gtctccctac tccagccagt cgcaggtgca
    16681 catccggcaa ttgctcactt tttacaacag cctaatgccg aacgtttaag cgaactgttc
    16741 gccagcctgt tgaatatgca gggtgaagaa aaatcccgcg cgctggcgat tttaaaatcg
    16801 gcgctcgaca gtcagcaggg tgaaccgtgg caaacgattc gtttaatttc tgaattttac
    16861 ccggaagaca gtggcctgtt ctccccgctg ttactgaatg tggtgaaatt gaatcctggc
    16921 gaagcgatgt tcctgttcgc tgaaacaccg cacgcttacc tgcaaggcgt ggcgctggaa
    16981 gtgatggcga actccgataa cgtgctgcgt gcgggtctga cgcctaagta cattgatatt
    17041 ccggaactgg ttgccaatgt gaaattcgaa gccaaaccgg ctaaccagtt gttgacccag
    17101 ccggtgaaac aaggtgcaga actggacttc ccgattccag tggatgattt tgccttctcg
    17161 ctgcatgacc ttagtgataa agaaaccacc attagccagc agagtgccgc cattttgttc
    17221 tgcgtcgaag gcgatgcaac gttgtggaaa ggttctcagc agttacagct taaaccgggt
    17281 gaatcagcgt ttattgccgc caacgaatca ccggtgactg tcaaaggcca cggccgttta
    17341 gcgcgtgttt acaacaagct gtaagagctt actgaaaaaa ttaacatctc ttgctaagct
    17401 tattaaaggc ttataacacc ttcaggcggc cagtccgcct gatttcattt tatggataat
    17461 cattatgaat aaatcgctgg tagcggtagg cgtcattgtt gcgctaggcg tagtctggac
    17521 aggcggcgca tggtatacag gcaagaagat tgaaacccat ctcgaagaca tggtcgcgca
    17581 ggcgaacgcg caactcaaac tgacagctcc tgaatccaac ctggaagtga gttatcaaaa
    17641 ctatcatcgc ggcgtattca gcagccagtt gcaactgttg gtgaaaccca ttgccgggaa
    17701 agaaaatccg tggattaaaa gcggtcagag cgtcatcttc aacgaatcgg ttgatcatgg
    17761 tcccttcccg cttgcccagc ttaaaaaact gaacctgatc ccgtcgatgg catcaattca
    17821 aaccacgctg gttaataacg aagtaagcaa accactgttt gatatggcaa aaggtgaaac
    17881 gccttttgag attaactcgc gcattggtta cagcggtgat tccagttccg atatttcgct
    17941 caagccactg aattacgagc aaaaggatga aaaagtcgcc tttagcggcg gcgagttcca
    18001 gttaaatgct gacagagacg gcaaagccat ctccctttcc ggggaggcgc aaagtggtcg
    18061 gatagacgca gttaacgaat acaaccagaa agtgcagttg acctttaata atctgaaaac
    18121 cgacggttcc agcacgctgg caagttttgg tgagcgcgta ggaaaccaaa aactgtcact
    18181 ggaaaaaatg accatttcag tggaaggcaa agaactggca ctgctggaag gcatggagat
    18241 cagcggtaaa tcggatctgg tcaatgacgg taaaacgatc aatagccaac tggattactc
    18301 gctaaacagc ctgaaggtac agaatcagga tctgggcagc ggcaagctga ctttaaaagt
    18361 cggccagatt gatggtgaag cctggcatca gtttagccag caatataacg cgcaaactca
    18421 ggcgctgctg gcacagccag aaattgccaa caatcccgaa ctttatcagg agaaagtgac
    18481 ggaagccttc tttagcgccc tgccgctgat gttgaaaggc gatccggtga ttactatcgc
    18541 gccgctaagc tggaaaaaca gtcagggtga aagtgcgctg aatctgtcgc tgttcctgaa
    18601 agatccggca acgactaaag aagcgccgca aacgctggcg caggaagtag atcgttcggt
    18661 taaatctctg gatgcgaaac tgaccattcc ggtggatatg gcaactgagt ttatgactca
    18721 ggtagcgaag ctggaaggtt atcaggaaga tcaagcgaaa aaactggcga aacagcaagt
    18781 tgaaggtgca tcagcaatgg ggcagatgtt ccgtctgacc accttgcagg acaataccat
    18841 caccaccagc ctgcaatata ctaacggtca gataacgtta aacgggcaga aaatgccact
    18901 ggaagatttc gttggtatgt ttgcgatgcc agctcttaac gttccggctg taccggcaat
    18961 tccgcagcag taattcacgg ccaaaacgcc ggagaatttt cacttcggcg ttttcatctg
    19021 aaaacaaacc agagattgcc tgatgcgcta cgcttatcag gcctaccttt tcctgcaaca
    19081 ctttgaattt atgagttttt gtaggctgga taaggcgttt acaccgcatc cggcataaaa
    19141 aacgcgcact ttgtcaacaa tctgaaacgc cggaggtttt tctctccggc gttatttctg
    19201 acttcagcat aaagtcatac tgaatactca ttttcacatc ccagtaattc aggcgatcgc
    19261 ccagcgtact gccatcgcgc ttttcccgcc ctttatcggc agcaagaaag aaattcatcc
    19321 cttgtaacgg cccttcgctg acccgatagt tcataccacc taagataccc gcgccttcaa
    19381 tccgcacatc tcgtttactg gattcatagc cgcgagtcac aatctgtgca aatgtcagtg
    19441 taaattgcgg cgttaaacga tagttgacgc ccaattgcca ggactgcatt ccttcacggt
    19501 tacgatcaag acttaatgaa aagggatagc caatgtcggt atccaccacg gcaccattca
    19561 ttgcatggcg caaataacca ccactgccaa atacgcttcc ccacggcgca ttccaggtca
    19621 attgtccact aaccaacgcc gtttcattgg gctggctggt attacgactt aaaccttcga
    19681 gctgcgcgta aaagccgagc aactcccctt tcaacgtata attcgggtcg agcggtatgc
    19741 ttttctcgag aatactttgt atctgattgg aaacatcttt ctgtaccgat gcccccagag
    19801 aatagcgact ggtatcatca tgccaactgg cggctaaaaa agaacgtgcg cgatgtttat
    19861 cgtattttcc cgtctggctg ttatacaccc cttcatcacg aataccggtt gttgaatcag
    19921 tccataaaaa tactcgtgaa cgccacattc cccacagatc aatatttggt gttaaagccc
    19981 aataacctaa tgctccttca gaaactgtcg gtaatgtacg ttgcgaactg ccagccatta
    20041 acggaatacg ataaggtaag ctaccataac tataatccgt cccgaagcga cctgatttaa
    20101 gcaggctatt ttcactgggt tttattttta ctgcccccag ggcaaagcca aaacttttat
    20161 caccatcaag ataccaccgg gtactcatat cagcacgagc acctaattta taaacataat
    20221 atgccccgcc ttcaacgcca ataatattgg aataataacc actattgaaa tcgagcaatc
    20281 ctccctgtac ccatgcgtaa atatctcttc ccgcgccagc actgggctta tccgccctgc
    20341 gcaattcgtt tttcagtctg atgcgtagtc ccgcctcatc gtcagccagt tgcgccgcat
    20401 aagcagagat aaggccagag gcagccgtca ggcaaataac ggccatggcc actatttttc
    20461 tcatagttgc acctttgatg ttctgattat ttttattgat tattaattag tgatatcgct
    20521 gattaattgc tgctgcactt ttttacgatt atcaatttca accacgattt ctttgaattt
    20581 tttatccgta agcggataaa accagataat aacgaatgcc agtagcataa atccgcaagg
    20641 tactaaggca attgatgtgc ggatgcccat aataacttcc ggcgtttgca cctgattggc
    20701 aatatatccg cttaacccca aaataaaggc aggaattgaa cctccgattg cctgaccgca
    20761 tttacgggta aatgagaata gtgaataggt gagcccttca attcgcacgc cggtcaggta
    20821 ttcaccgtat tctacggtat cagcttccag tgcccacatc actgtcatag taacgccctg
    20881 accaattgaa gcgatggcca acgcaaccaa cgccaccggc agtgaccaga cggaaaccca
    20941 gaagaatagc agataaccgc aggttcccag caaagcgcca atcaggaagg tattcttttt
    21001 accgatcctc gcgaccatcc ccggcacaag cggtgccgat gccacagtac caaccaggtt
    21061 ttgcaccagt accagcacag tgaacagccc ggtatcattt aacacatagc gcacgtagaa
    21121 caacgacgag gcgctgaccg caaaggtcga aatcagcaca cacagcgcac cgatgcacaa
    21181 cataaacagc gggcgattcc gtttcagggt ttgcaaactg atcttcaatg acggctgcgc
    21241 aacgatacgt accacattct cacgcgtcga tttgaagcag atgaagtaaa gcaccattcc
    21301 ggcaatcgcc agcacaatcg tccagaaatg gtataccgac accatctctt ccgggctgga
    21361 gttcttaatg ctcggcccta tcagaaatgc caggcagaca aaggtcaatg aagcggcaat
    21421 cccacgagcc gcgcccagac gggcgcggga ttgtggttgt tgggtcatcg cggtagcaag
    21481 tgaaccataa ggaatattca ccaggctgta gcaaaacccg aggcccatgt aggtcaaata
    21541 tgcatacact actttgcggc tatggctcca gtcggtcggt acccagaata ccagcacgct
    21601 gaagatcatt aacggcgcag taccgaagag taaaaacggg cggaattttc cccagcgggt
    21661 attcacactg tccaccactc gtccggcaaa gacgtcggcg aaggcatcga atacccgcac
    21721 cagtaacagc atggtgcccg ccgcagcggc accgacgcca gcgacgtcgg tgtagtaact
    21781 caacaggaag agcaccccca ttgcgaaggc gaagttattg gcgacgtcac cgaggctgta
    21841 gccgacgatg gtgcgccagg agagttgttg attcattgtt tgcctccctg ctgcggtttt
    21901 tcaccgaagt tcatgccagt ccagcgtttt tgcagcagaa aagccgccga cttcggtttg
    21961 cggtcgcggg tgaagatccc cttcttgtta ccgccaacgc gcaatatgcc ttgcgaggtc
    22021 gcaaaatcgg cgaaattcca tacctgttca ccgacgacgg cgctgacgcg atcaaagacg
    22081 cggtgataca tatccagcca tgcacactga tactcttcac tccacatgtc ggtgtacatt
    22141 gagtgcagcc cggctaacgt atccacgccg tattcggtga tgataatcgg ctgatgcagt
    22201 ttctcctgcc aggccagaag ttctttttcc agtaccttct ctgccgtttc caaatcgccg
    22261 ctttggacat accatccgta ataacggttc aggcacagca catcaaagag atcgctgatg
    22321 gtatcggtgt gagcgtcgca gaacattaca ttgacgcagg tgatcggacg cgtcgggtcg
    22381 agtttacgcg ttgcttccgc cagtggcgcg aaatattccc gtgcaccttg cggacgggta
    22441 tccggttcgt tggcaatact ccacatcacc acgcttgggt ggtttttgtc acgcgctatc
    22501 agctctttaa tcgcctgtaa gtgcgcttgc tgagtttccc cgttgactgc ctcttcgctg
    22561 tacagttctt tcggcttgtt gcccgcttcg aaaccaatgc ctaaagagag gttaaagccg
    22621 acagcagcag tttcatcaat caccacgatg ccatgttcat ctgcccagtc gagcatctct
    22681 tcagcgtaag ggtaatgcga ggtacggtag gagttggccc caatccagtc cattaatgcg
    22741 tggtcgtgca ccatcagcac gttatcgaat cctttgccac gcaagtccgc atcttcatga
    22801 cgaccaaagc cagtaaagta gaacggtttg tggttaatca ggaactgttc gcccttcact
    22861 gccactgacc ggatgccgac gcgaagcggg tagatatcac actctgtctg gcttttggct
    22921 gtgacgcaca gttcatagag ataaccttca cccggttgcc agaggtgcgg attcaccact
    22981 tgcaaagtcc cgctagtgcc ttgtccagtt gcaaccacct gttgatccgc atcacgcagt
    23041 tcaacgctga catcaccatt ggccaccacc tgccagtcaa cagacgcgtg gttacagtct
    23101 tgcgcgacat gcgtcaccac ggtgatatcg tccacccagg tgttcggcgt ggtgtagagc
    23161 attacgctgc gatggatccc ggcatagtta aagaaatcat ggaagtaaga ctgctttttc
    23221 ttgccgtttt cgtcggtaat caccattccc ggcgggatag tctgccagtt cagttcgttg
    23281 ttcacacaaa cggtgatacg tacacttttc ccggcaataa catacggcgt gacatcggct
    23341 tcaaatggcg tatagccgcc ctgatgctcc atcacttcct gattattgac ccacactttg
    23401 ccgtaatgag tgaccgcatc gaaacgcagc acgatacgct ggcctgccca acctttcggt
    23461 ataaagactt cgcgctgata ccagacgttg cccgcataat tacgaatatc tgcatcggcg
    23521 aactgatcgt taaaactgcc tggcacagca attgcccggc tttcttgtaa cgcgctttcc
    23581 caccaacgct gatcaattcc acagttttcg cgatccagac tgaatgccca caggccgtcg
    23641 agttttttga tttcacgggt tggggtttct acaggacgta acataaggga ctcctcatta
    23701 agataaaaat actggtcaac ctttaatctg attagttaac caattctgtg ttgcacacag
    23761 ttatctgtga gaaacccatg gaagagaaat ggaaataaaa agaactgaca ccgcgatcaa
    23821 aaaaccaaaa actgcaatac ccacagcgtg atattgatcg cattaatctt taaaacggcg
    23881 ttgacagaag ccataaaata agcaaccgaa cgttcgttcg agcatattgg gaacgataaa
    23941 ctctcatgac atatggctac ggtgaatatt tttggggggg aattctccga tcccggctag
    24001 attgtatttt gtacagggaa attaaagtat gaaacgcggg cacagttatc accagcccgc
    24061 gaaatgagag gatcaggatg cggttaagat accgccaatc atcgcgcgta atccctgagc
    24121 aaacgacgct tcacgcgcca gatcttccgc ttcgatatcg gccagtacac cgtaggtcag
    24181 atccagcagt aaacgtgaag tcattgacgt gttgatgtct gggcttatct cgcctttttg
    24241 ctgggcatca cgcatccgct gggcaacaaa ttccgtgatc gtcatatgct tatttttaag
    24301 catggcggcg acctgtgggt tacgcatccc ttccgccata atttcgacca ccagcgcccg
    24361 ttgcccaaag gcttcatggg tgagagagac aatagactcg accatatagt caacaaaatg
    24421 aatcccttta atcggttccc ggaaacgagc cagcgccctc tcctggtcct gtaagataat
    24481 cgcctgaatc aaggcttctt tggagatgaa atggtgatag agcgtcccgg gactaatggc
    24541 gcaagattta cagatggctt tcatcgaggc actgtgaaat ccattttctg aaaaaatctc
    24601 tctggcagca ttgaggatcc gggtccgtgt cggttgtgct tcagtctgca tgttatccat
    24661 cattaaacca ttatagttag cgtgaataac tagtttaccg cgttgtcagg tgagcgccta
    24721 tcattgctgt tgaatagtgc gaaggcacac tctattcgta tacttttcac tctgaaattg
    24781 gttaaccaat agccgtgatt tggctgccag ttatttagca gagacgttga tgagtttatc
    24841 ccggtaaaca acacgcacgg ttgtttaccg gtccgttagt gtattaattg agctcctgta
    24901 ccccaccacc ggagacggtg agaatttgtc cgcttaccca gctcgcagca ggcgagcaaa
    24961 ggaacagcgc tgcgttagca atatcttgcg gttggcccag acgtctgatc ggcgtgtgct
    25021 gtaacatttt ttgttcaatt tctggtgtaa taacggattt cagggcatcg gttaatattg
    25081 cccccggcgc aatgccattt acccgaatat ttttttcacc caggtcaaac gccatatttc
    25141 tgaccagatg actggctgca gctttagatg atgcatagga agtcatgttt atatttttat
    25201 tttctgccgc catagaagtg atggtcagaa taacgccacc gccatttttt tccatttctg
    25261 gcgcaacaag ttgtgacaga tggaaaaaag aaaacacatt cagttcataa gcacggcgaa
    25321 aatccgccat tggcatatca aacggtttag gtccaccgcc accggcgttg ttaaccagaa
    25381 tatcaacttt acccagctta ctgatagcaa agtctgccag tgcagagagt tcctgttcgg
    25441 aagtaatatc acaacggcag gcaaatgcct gaccacccag ttgttgaatt tcgtctacaa
    25501 catggttagc tgcgtcggcg ttaatatcac tgaccaccac agatgcgcca gctgtcgcga
    25561 atgtaatggc gatttcttta ccaatacctg cacccgcacc tgtgatgatg gcgcatggcg
    25621 catggcgcat ggcgcatttt ccgtc
//
LOCUS       DAJVQG010000047        25496 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJVQG010000047 DAJVQG010000000
VERSION     DAJVQG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25496
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.047"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            317..871
                     /locus_tag="OQM83_001732"
     CDS             317..871
                     /locus_tag="OQM83_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCS1520014.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            complement(847..1104)
                     /locus_tag="OQM83_001733"
     CDS             complement(847..1104)
                     /locus_tag="OQM83_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCS1520015.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(1101..1919)
                     /gene="aroE"
                     /locus_tag="OQM83_001734"
     CDS             complement(1101..1919)
                     /gene="aroE"
                     /locus_tag="OQM83_001734"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCS1520016.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            complement(1924..2496)
                     /gene="tsaC"
                     /locus_tag="OQM83_001735"
     CDS             complement(1924..2496)
                     /gene="tsaC"
                     /locus_tag="OQM83_001735"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCS1520017.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            complement(2501..3043)
                     /locus_tag="OQM83_001736"
     CDS             complement(2501..3043)
                     /locus_tag="OQM83_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCS1520018.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            complement(3072..3545)
                     /locus_tag="OQM83_001737"
     CDS             complement(3072..3545)
                     /locus_tag="OQM83_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCS1520019.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            complement(3517..4641)
                     /gene="dprA"
                     /locus_tag="OQM83_001738"
     CDS             complement(3517..4641)
                     /gene="dprA"
                     /locus_tag="OQM83_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCS1520020.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            4771..5280
                     /gene="def"
                     /locus_tag="OQM83_001739"
     CDS             4771..5280
                     /gene="def"
                     /locus_tag="OQM83_001739"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCS1520021.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            5295..6242
                     /gene="fmt"
                     /locus_tag="OQM83_001740"
     CDS             5295..6242
                     /gene="fmt"
                     /locus_tag="OQM83_001740"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCS1520022.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            6288..7577
                     /gene="rsmB"
                     /locus_tag="OQM83_001741"
     CDS             6288..7577
                     /gene="rsmB"
                     /locus_tag="OQM83_001741"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCS1520023.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            7599..8975
                     /gene="trkA"
                     /locus_tag="OQM83_001742"
     CDS             7599..8975
                     /gene="trkA"
                     /locus_tag="OQM83_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCS1520024.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            9105..9515
                     /gene="mscL"
                     /locus_tag="OQM83_001743"
     CDS             9105..9515
                     /gene="mscL"
                     /locus_tag="OQM83_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCS1520025.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(9512..9730)
                     /gene="arfA"
                     /locus_tag="OQM83_001744"
     CDS             complement(9512..9730)
                     /gene="arfA"
                     /locus_tag="OQM83_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HCS1520026.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(9786..10211)
                     /gene="zntR"
                     /locus_tag="OQM83_001745"
     CDS             complement(9786..10211)
                     /gene="zntR"
                     /locus_tag="OQM83_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HCS1520027.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            complement(10222..10590)
                     /locus_tag="OQM83_001746"
     CDS             complement(10222..10590)
                     /locus_tag="OQM83_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HCS1520028.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            complement(10698..11081)
                     /gene="rplQ"
                     /locus_tag="OQM83_001747"
     CDS             complement(10698..11081)
                     /gene="rplQ"
                     /locus_tag="OQM83_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCS1520029.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            complement(11122..12111)
                     /gene="rpoA"
                     /locus_tag="OQM83_001748"
     CDS             complement(11122..12111)
                     /gene="rpoA"
                     /locus_tag="OQM83_001748"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCS1520030.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            complement(12137..12757)
                     /gene="rpsD"
                     /locus_tag="OQM83_001749"
     CDS             complement(12137..12757)
                     /gene="rpsD"
                     /locus_tag="OQM83_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCS1520031.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            complement(12791..13180)
                     /gene="rpsK"
                     /locus_tag="OQM83_001750"
     CDS             complement(12791..13180)
                     /gene="rpsK"
                     /locus_tag="OQM83_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCS1520032.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            complement(13197..13553)
                     /gene="rpsM"
                     /locus_tag="OQM83_001751"
     CDS             complement(13197..13553)
                     /gene="rpsM"
                     /locus_tag="OQM83_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCS1520033.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            complement(13700..13816)
                     /gene="rpmJ"
                     /locus_tag="OQM83_001752"
     CDS             complement(13700..13816)
                     /gene="rpmJ"
                     /locus_tag="OQM83_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCS1520034.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            complement(13848..15179)
                     /gene="secY"
                     /locus_tag="OQM83_001753"
     CDS             complement(13848..15179)
                     /gene="secY"
                     /locus_tag="OQM83_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCS1520035.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            complement(15187..15621)
                     /gene="rplO"
                     /locus_tag="OQM83_001754"
     CDS             complement(15187..15621)
                     /gene="rplO"
                     /locus_tag="OQM83_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCS1520036.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            complement(15625..15804)
                     /gene="rpmD"
                     /locus_tag="OQM83_001755"
     CDS             complement(15625..15804)
                     /gene="rpmD"
                     /locus_tag="OQM83_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCS1520037.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            complement(15808..16311)
                     /gene="rpsE"
                     /locus_tag="OQM83_001756"
     CDS             complement(15808..16311)
                     /gene="rpsE"
                     /locus_tag="OQM83_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCS1520038.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            complement(16326..16679)
                     /gene="rplR"
                     /locus_tag="OQM83_001757"
     CDS             complement(16326..16679)
                     /gene="rplR"
                     /locus_tag="OQM83_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCS1520039.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            complement(16689..17222)
                     /gene="rplF"
                     /locus_tag="OQM83_001758"
     CDS             complement(16689..17222)
                     /gene="rplF"
                     /locus_tag="OQM83_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCS1520040.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            complement(17235..17627)
                     /gene="rpsH"
                     /locus_tag="OQM83_001759"
     CDS             complement(17235..17627)
                     /gene="rpsH"
                     /locus_tag="OQM83_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCS1520041.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            complement(17661..17966)
                     /gene="rpsN"
                     /locus_tag="OQM83_001760"
     CDS             complement(17661..17966)
                     /gene="rpsN"
                     /locus_tag="OQM83_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCS1520042.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            complement(17981..18520)
                     /gene="rplE"
                     /locus_tag="OQM83_001761"
     CDS             complement(17981..18520)
                     /gene="rplE"
                     /locus_tag="OQM83_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCS1520043.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            complement(18535..18849)
                     /gene="rplX"
                     /locus_tag="OQM83_001762"
     CDS             complement(18535..18849)
                     /gene="rplX"
                     /locus_tag="OQM83_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCS1520044.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            complement(18860..19231)
                     /gene="rplN"
                     /locus_tag="OQM83_001763"
     CDS             complement(18860..19231)
                     /gene="rplN"
                     /locus_tag="OQM83_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCS1520045.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            complement(19396..19650)
                     /gene="rpsQ"
                     /locus_tag="OQM83_001764"
     CDS             complement(19396..19650)
                     /gene="rpsQ"
                     /locus_tag="OQM83_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCS1520046.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            complement(19650..19841)
                     /gene="rpmC"
                     /locus_tag="OQM83_001765"
     CDS             complement(19650..19841)
                     /gene="rpmC"
                     /locus_tag="OQM83_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCS1520047.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            complement(19841..20251)
                     /gene="rplP"
                     /locus_tag="OQM83_001766"
     CDS             complement(19841..20251)
                     /gene="rplP"
                     /locus_tag="OQM83_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCS1520048.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            complement(20264..20965)
                     /gene="rpsC"
                     /locus_tag="OQM83_001767"
     CDS             complement(20264..20965)
                     /gene="rpsC"
                     /locus_tag="OQM83_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCS1520049.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            complement(20983..21315)
                     /gene="rplV"
                     /locus_tag="OQM83_001768"
     CDS             complement(20983..21315)
                     /gene="rplV"
                     /locus_tag="OQM83_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCS1520050.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            complement(21330..21608)
                     /gene="rpsS"
                     /locus_tag="OQM83_001769"
     CDS             complement(21330..21608)
                     /gene="rpsS"
                     /locus_tag="OQM83_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCS1520051.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            complement(21625..22446)
                     /gene="rplB"
                     /locus_tag="OQM83_001770"
     CDS             complement(21625..22446)
                     /gene="rplB"
                     /locus_tag="OQM83_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCS1520052.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            complement(22464..22766)
                     /gene="rplW"
                     /locus_tag="OQM83_001771"
     CDS             complement(22464..22766)
                     /gene="rplW"
                     /locus_tag="OQM83_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCS1520053.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            complement(22763..23368)
                     /gene="rplD"
                     /locus_tag="OQM83_001772"
     CDS             complement(22763..23368)
                     /gene="rplD"
                     /locus_tag="OQM83_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCS1520054.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            complement(23379..24008)
                     /gene="rplC"
                     /locus_tag="OQM83_001773"
     CDS             complement(23379..24008)
                     /gene="rplC"
                     /locus_tag="OQM83_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCS1520055.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            complement(24041..24352)
                     /gene="rpsJ"
                     /locus_tag="OQM83_001774"
     CDS             complement(24041..24352)
                     /gene="rpsJ"
                     /locus_tag="OQM83_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCS1520056.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            complement(24590..25009)
                     /gene="gspB"
                     /locus_tag="OQM83_001775"
     CDS             complement(24590..25009)
                     /gene="gspB"
                     /locus_tag="OQM83_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HCS1520057.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVKESDEKAGLRERVKNALNELER"
     gene            complement(25011..25481)
                     /locus_tag="OQM83_001776"
     CDS             complement(25011..25481)
                     /locus_tag="OQM83_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HCS1520058.1"
                     /translation="MVDQNLPWSASLKVGDKKLPVVVVRVGEASVDVLVGQQTWTLTH
                     KWFESVWTGDYLLLWKMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPL
                     LVEKIKQFQKSHHLKTDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
BASE COUNT         6301 a         6680 c         6023 g         6492 t
ORIGIN      
        1 ctttcagctc actgtcgcga ggtggcgtat attacgcttt cctctttcag agtcaaccct
       61 gaatttcaga attttttctc ttcgttgttc cgaccatcct gtgaagtgtt tcacgttgtc
      121 gtctcaacgg aggcgcatta tagggatccc aattttttgc acaagtactt ttttgatctt
      181 tttttctgtt tgttgttttt tcaccctttt tgctgcattc gcacacaaaa cggtgctttt
      241 ttgcatacta aaagacttgc acaaggctaa taatgcaccc aaagtcatta ggaaatcatt
      301 tattgttgag gtgagtatgt ctgatgtttt acgcccatac cgcgatcttt ttccacaaat
      361 cggccagcgc gtaatgatcg acgatagcag cgtcgtgatt ggtgacgttc gtctggctga
      421 tgatgtgggg atctggccgc tcgttgtgat tcgtggagat gtacattatg tacagatcgg
      481 agcacgcacc aatatccagg atggcagtat gttgcatgtc actcataaat cctcgtacaa
      541 cccagatggc aacccattaa ccattggcga agatgtcact gttggtcaca aggtgatgct
      601 ccacggctgc accattggca atcgagtttt ggttgggatg ggctcaattt tacttgatgg
      661 cgcaatagta gaagatgatg tgatgattgg tgcgggtagc ctggtcccac aaaataaacg
      721 gctggagagc ggatatctgt atctcggtag tcccgtcaaa cagatccgcc cgttaagtga
      781 tgaagagaag gctgggttac gctattccgc aaataactac gtgaaatgga aggacgaata
      841 tctggatcaa ggtaaccaga cccaaccttg atcatcttca ctttgttcct gaattaagtt
      901 ttccgcttct tcttccaggt cccagcgatg ctgacgaaaa ctcgctaacc actgttctgg
      961 cgtacctcca gtaaagcgat acgccagact ctcgccagag atcgcacatg tcagttgcat
     1021 accattcacg agagcaggaa aacatacaca tcttttattc tcgtcccact cttccctatc
     1081 cggaaactgg atggcctgat tcacgctgac aattcctgtt gcaatagttt gatgaccggt
     1141 tctacatcag gcagaacgcc atgccaaaga aggaaggcat gagccgcctg tgccaccagc
     1201 attcctaaac catcagcatt acgctttgag cctcgctgct cacaccatgc cagaaaagga
     1261 gtttttcctt tctgatagaa catgtcatag caataaatgc ctggatgaat gagcgatgac
     1321 gggatcgccg gaatatcacc actgatgcca ctggatgttg cattaataat gagatcaaac
     1381 tcatgacctt ccagttcgtc catacccaac gcctgaatac tgccagtgtg cgcaaacaat
     1441 ttagccaact cttccgcgcg ggataccgtc cgattagtga ttgtcaccgc acagtctagg
     1501 gaaagaagtg gcagtagtac gccgcgagat gctccaccag cgccgataag cagaatacgt
     1561 aaaccagggc ggataaaaga cagacgttcc agatcgctta acaagcctac accatcggta
     1621 ttgtcaccca gcaggcgtcc atcttctaac cgcttgaggg tattaactgc accagccaac
     1681 gctgcccgtt cagtaagctc atccgctctg gcaaaagcct cttctttaaa aggcaccgtc
     1741 acattcgcac ctttaccacc agcacgaaag aaagcgttca gtgtgttgat gaaatcattg
     1801 atgggtgcca acacgcgccc atagggatgt tcaatattca gttgctgagc aaattgctga
     1861 tgaatgaatg gcgatttgct gtgggctatc ggattaccaa aaacagcata ggtttccatt
     1921 atgttacccc tgtcgaaaca gttcacccgt cagggcatcg cggatttctg aaggatttaa
     1981 acgccccccc gtttcaccag gcacaaccgg gaacgccgcg ccaaattgtg cgcgaacttc
     2041 gtctactgtt cgacaaggtg gcaatccact caagttggca ctggtagaaa ccagcggttt
     2101 accataagcc tggcacaaag caaccaccaa cggatggtcg gtgactcgta cagcaagcga
     2161 atcaaagcgg cccgtcaacc agcgcggtgt tgtcgcaggc gcgggaaaga caaaggtgac
     2221 aggacctggc cagcgggaaa aaatggtttc acgctgcgca tcagtcagca tggtgtcatc
     2281 aatatagggt ttaagctgct cgtaatttgc cgcgattaaa atcagcccct tatcaaccgg
     2341 acgctgtttt aactccaaca gtctcatcac tgctgtttcg ctatcaggat cgcacccaac
     2401 accgaaaacg gcttccgttg gataggcgat gacacgttct tcattgagga catctatcgc
     2461 agctgcgata gcgtctcctt gcaggttatt attcacgtta ttattccgcc gaaaccggct
     2521 ttccacattg tttactggca caaaagtgtt ttacaccctg cgcggttttc ttttcgatga
     2581 gtagcggata atgacactca gggcattctc cagctattgg tttgaagtta atggcaaatt
     2641 gacactccgg gtagcgatca caggagtgaa atgttttgcc ataacgggag cggcgctgga
     2701 ccagatggcc cgtccgacat tgggggcatg taattgctgt ttcgtccggt ttatcgataa
     2761 gttcggtatg ttcgcattca gggtagttac tgcaaccaat aaacatacca aagcgtccct
     2821 ggcgtaatac cagatttgcg ccacatgcag ggcaaacctg cccctccaga actttgacaa
     2881 tatgtccatc cgctgaagat ttcagaagac ggacgtagtc acacgccgga tactgtgagc
     2941 atccaagaaa cggaccgtgt ttcccggatc gaataaccag ttcagccccg cactttgggc
     3001 aggactcatt attacgcacc gtgaacagtg ctgattttgc cataacaact tatgctgaat
     3061 taaagaaatg attaatgcag cataccttca ttcacttcaa agagtaattc ttccatttgc
     3121 tggtacgcat tttcgcaccc cgggatgttg aacaacacca tcaggatcac ccatttcaga
     3181 tcatccagtt cgaactctgc gttatccagc gcgagcactc gctctatcac catttcacga
     3241 gtttcaaggt tgagcacctg aatctgctca aggaaaagca gaaacccacg gcagctggca
     3301 tccagtcgtt cacactcttc cggtgtataa atacgcatgg agagaggatc cgaggccagt
     3361 tgcatcggtt ctgccaaccc ttcctgataa tcagcaagtt tttccagcca tagcagggca
     3421 ttgtagatat cttctcgctc aaaacctgcg tcggtaagat cctgttcaag tttgtcttga
     3481 tccacacgca actcagcttc agtgtgaata taggtttcaa acaaatacat tagtacgtcg
     3541 aacatggcat gccctcctca atcggacata gccgccgggt acagctgcga tccatcctgc
     3601 taactccagt tcgagtagtt gagtaactac ctctggcaca ggttggccgg cacgttcagc
     3661 gacgacgtca acaggtgtta cctcatctcc tacgttagcc aggagctcag gaaatggcaa
     3721 tgccacgtct tgctgatctg gtgaataaaa tgaattttca ggggcgtctg gcaaccagtg
     3781 caatccaaat tgcaagtttt ccagaatttc ttccggttcc gtcacaagaa tcgcaccttg
     3841 ttttattaac cagtgaggcc cttcgcttcc cggattccct attggacctg gcaaggcaaa
     3901 aacttcacgc ctctgctcaa gcgcacaacg tgctgtcacc agcgaaccac tgcgcaaagc
     3961 cgcttccacc accagtacac ctttacttag accactgata atgcgatttc ttcgtgggaa
     4021 attgtaagca aggggtggaa catcgagggg aaattccgag acgagagctc cgccatgttc
     4081 aagcagactg gcagccagtc gggcatggcg gcggggatga atggtattaa gtccattccc
     4141 caatacagca atgctgacgc catttacctg taaggcagct ttatgcgcca caccatcgat
     4201 tccacgcgcc agtccactcg taattgtcac tccacgcgtc gccagagttt cgcaaaataa
     4261 ccgtccccat cgctcgccat accatgaatg cgcccgactc cccactacgg caagctgaaa
     4321 tgaatgcagc gcgtgcagtt ctccttcaac aaacagtgcg ccggggtaat ctgtcgtcac
     4381 cagaagttga ggaggataaa attcgctgtc cgcagggatt aaatgatggt tgggttgctc
     4441 caaccaacaa agtgagcttt cgatactctt tcgcggaaat gaaagaaagc gttgtgcctg
     4501 ccgcaatgta agccctgttt gctgcaatcc aaccgcatca atttgcgact gttttgccag
     4561 ccagtgagct atacggacca tatcatcgcc gtacaagctg ctgatactca ttaaacgcag
     4621 ccaaatttct gtatcgacca tccttatctc cctgccataa gcagccttag caatctttgc
     4681 gattggtcag tgatgctgtc aatcagaggg ggatttgtct agaatagaag aaataatctt
     4741 tctaacccct gaacacatct ctggagattt atgtcagttt tgcaagtgtt acatattccg
     4801 gacgagcggc ttcgcaaagt tgctaaaccg gtagaagaag tgaatgcaga aatacagcgt
     4861 atcgtcgatg atatgttcga gacgatgtac gcagaagaag gtattggcct ggcggcaacc
     4921 caggttgata tccatcaacg tatcattgtt attgatgttt cggaaaaccg tgacgaacgg
     4981 ctggtgttaa tcaatccgga gcttttagaa aaaagcggcg aaacaggcat tgaagaaggt
     5041 tgcctgtcga tccctgaaca acgtgcttta gtgccgcgcg cagagaaagt taaaattcgc
     5101 gcccttgacc gcgacggtaa accatttgaa ctggaagcag aggggctgtt agccatctgt
     5161 attcagcatg agatggatca cctggtcggc aaactgttta tggattatct gtcaccgctg
     5221 aaacaacaac gtattcgtca gaaagttgaa aaactagatc gtctgaaagc ccgggcttaa
     5281 ggataagaac taacgtgtca gaatcactac gtattatttt tgcgggtaca cctgactttg
     5341 cagcgcgtca tctcgacgcg ctgttgtctt ctggtcataa cgtcgttggc gtgttcaccc
     5401 agccagaccg accggcagga cgcggtaaaa aactgatgcc cagcccggtt aaagttctgg
     5461 ctgaggaaaa aggtctgccc gtttttcaac ctgtttccct gcgtccacaa gaaagccagc
     5521 aactggtcgc cgatctgcag gctgatgtta tggtcgtcgt cgcctatggc ttaattctgc
     5581 ctaaagcagt gctggagatg ccgcgtcttg gctgtatcaa cgttcatggt tcactgctgc
     5641 cacgctggcg cggtgctgca ccaatccaac gctcactatg ggcgggtgat gcagaaactg
     5701 gtgtgaccat tatgcaaatg gatgtcggtt tagacaccgg tgatatgctc tataagctct
     5761 cctgcccgat tactgcagaa gataccagtg gtacgctgta cgacaagctg gcagagcttg
     5821 gcccacaagg gctaatcacc acgttgaaac aactagcaga cggcacggcg aaaccagaag
     5881 ttcaggacga aactcttgtc acttacgccg agaagttgag taaagaagaa gcgcgtattg
     5941 actggtcgct ttcggcagcg cagcttgaac gctgcattcg cgctttcaac ccatggccaa
     6001 tgagctggct ggaaattgaa ggacaaccgg ttaaagtctg gaaagcgtcg gtcattgata
     6061 cggcaaccaa cgctgcacca ggaacgatcc ttgaagccaa caaacaaggc attcaggttg
     6121 cgactggtga tggcatcctg aacctgctct cgttacaacc tgcgggtaag aaagcgatga
     6181 gcgcgcaaga cctcctgaat tctcgtcggg aatggtttgt tccgggcaac cgtctggcct
     6241 gatagtccac tcttctaagc ccggtcttac cgggcatttt tatacttatg aaaaaacaac
     6301 gtaatttacg tagcatggcg gcccaggccg ttgaacaagt cgtcgagcaa gggcaatcat
     6361 taagcaacat tctgccaccg ctccagcaaa aagtttctga taaagacaaa gcacttcttc
     6421 aagagttgtg ctttggcgta ctgcgtacgc tttcgcagtt agactggctg attaataagt
     6481 taatggcccg tccgatgacc ggcaaacagc gcactgtgca ttacctgatt atggttggtt
     6541 tgtatcaact gctttatacc cgcattccac ctcatgctgc gctggctgaa acggttgaag
     6601 gcgctatcgc aattaagcgt ccgcaactta aagggttgat taacggtgta ttacgccagt
     6661 tccagcgtca gcaagaagag ttattagccg agtttaatac cagtgatgca cgttatctgc
     6721 atccttcctg gttgctgaag cgtctgcaaa aagcgtatcc agagcagtgg caatccatcg
     6781 tcgaagccaa taaccagcgt ccgccaatgt ggctgcgcgt taatcgtacg catcattccc
     6841 gcgacagctg gcttgcattg ttggatgaag caggaatgaa aggtttcccg catgcggatt
     6901 accctgatgc tgtacggctg gaaacacctg cacctgttca tgcgctacct ggttttgaag
     6961 acggatgggt taccgttcag gatgcatcag cacaaggttg catgacctgg cttgcgccac
     7021 aaaacggtga acacattctg gatctttgtg ccgcccccgg cggtaaaaca acgcatatcc
     7081 ttgaggtggc accagaagcg caggttgttg cggttgatat tgacgaacag cgcctctctc
     7141 gcgttcacga caatttaaaa cgccttggta tgaaggcgac cgtgaaacaa ggtgatggcc
     7201 gttacccttc ccaatggtgt ggcgagcaac agtttgatcg cattttatta gatgcgcctt
     7261 gttcagcaac gggggtgatt cgtcgccatc cggatattaa atggttacgt cgcgatcgcg
     7321 atatcccgga actcgcgcaa ttgcagtctg aaattctcga cgccatttgg ccgcatttaa
     7381 aatccggtgg aaccctggtc tatgccacct gttcggtgtt accggaagag aatagcctgc
     7441 agattaaagc ttttttgcaa cgtaccgctg atgccgaact ttgcgaaaca ggaacaccag
     7501 agcaaccggg taaacaaaat ctgcctggtg ccgaagaggg cgacggcttc ttttacgcta
     7561 agctaatcaa aaagtgatga gataacgggt cgagactgat gaaaattatc attctgggtg
     7621 ccggccaggt tggcggcaca ctggcggaaa acctggttgg cgagaacaac gatattactg
     7681 ttgtcgatac caacggtgag cgtctgcgga ccttgcagga taaatttgac ctgcgagtcg
     7741 tgcaggggca tggctctcat ccacgcgtat tgcgggaggc aggtgccgac gacgccgata
     7801 tgctggttgc tgtaaccagt tcagatgaaa ccaatatggt tgcctgccag gtagcctact
     7861 cacttttcaa cacccctaat cgcatcgctc gcatccgctc accagactac gtacgcgatg
     7921 ccgataagct atttcattca gatgctgtac cgattgatca tctgatcgca ccagagcagt
     7981 tggttatcga taatatttac cgactgattg agtatccagg cgcattgcag gtggtgaact
     8041 tcgctgaggg taaagtcagc ctcgctgtag ttaaagccta ttatggcggc ccgctgattg
     8101 gtaatgcact ttcgaccatg cgcgaacata tgccacatat cgatactcgt gtggcagcaa
     8161 ttttccgcca cgatcgcccc attcgtccac aaggttcgac cattgttgaa gctggtgatg
     8221 aagtgttctt tattgccgct tcacagcata tccgcgcggt gatgagtgaa ttacagcgac
     8281 tggaaaaacc gtataagcgg atcatgctgg ttggtggcgg taatatcggt gcagggctgg
     8341 cgcgtcgtct ggaaaaagat tacagcgtta aactcatcga acgtaatcag cagcgcgctg
     8401 ccgaactggc ggaaaagttg cagaatacga tcgtcttttt tggtgatgcg tcggatcaag
     8461 aactgctggc cgaagaacat atcgatcaag ttgatctgtt tattgccgtc accaacgatg
     8521 acgaggccaa tatcatgtcc gccatgcttg ccaaacgtat gggtgcgaaa aaggtgatgg
     8581 tattgatcca gcgtcgcgct tatgtggatc tggttcaggg gagcgtgatc gatattgcaa
     8641 tttcaccaca acaagcaact atttctgcgt tgcttagcca tgtgcgaaaa gcagatattg
     8701 ttggtgtttc ctcattgcgc cgcggcgtag cagaagctat tgaagccgtt gctcacggtg
     8761 atgaaagcac ctcacgcgtt gtcggcagag tcattgacga aatcaagcta ccgccaggaa
     8821 cgattattgg agcggtggta cgtggaaacg acgtgatgat tgccaatgac aatctgcgca
     8881 ttgagcaagg cgatcacgta attatgttcc tcacagataa aaagtttatt accgacgtcg
     8941 aaagactctt ccagccaagc cctttcttct tgtaattaat aaggcgtcta atgacgcctt
     9001 attatttccc tttgattatc aaggattaat taaattcatt cctggcagga aaatggctta
     9061 acatttgtta gacttatggt tgtcggcttc atagggagaa taacatgagc attattaaag
     9121 aatttcgcga atttgcgatg cgcgggaacg tggtggattt ggcggtgggt gtcattatcg
     9181 gtgcggcatt cgggaagatt gtatcttcac tggttgccga tatcatcatg ccacctctgg
     9241 gcttattaat tggcgggatc gactttaaac agtttgctgt cacgctacgc gatgcgcagg
     9301 gggatatccc tgctgttgtg atgcattacg gtgtcttcat tcaaaacgtc tttgattttc
     9361 tgattgtggc ctttgccatc tttatggcga ttaagctaat caacaaactg aatcggaaaa
     9421 aagaagaacc agctgccgca cctgcaccaa ctaaagaaga agtattactg gcagaaattc
     9481 gtgatttgct gaaagagcag aataaccgct cttaacaagc gcctgaaagc agaagaccag
     9541 tggtaaaaaa gtgatttact ttcttgccac tggcctccca gttcccccga ttgccatgtt
     9601 ttccttttcg catgtaactg cctttccctt tcttattttt ctctactcgc tgtcggaata
     9661 agggatcatg tagtaatgct tctatcgcat tatcctttat ctgcccttta gtatgctgat
     9721 atcggctcat aaaaactcca gttggttatt taacggcgcg agtgtaatcc tgtcagtgca
     9781 aaaaatcaac agccactctt aacgccactc gccccttgtt caagagcttc aagaatcgag
     9841 caataaacac tgctatgggc ggtcccacaa caggcatcgt taaggcgttg caaggaacgc
     9901 tgcatactct gcaactcggc tatccgtgct tcgacttcct gcaatctttc ctgcacaatg
     9961 ccttttgact cctgacaggt atggtgttca ggatcgatgc ggatcgacag caactcgcgg
    10021 atcgactcca gactgaaacc tagttgtctg gcatggcgga taaatttcaa tcgctggaga
    10081 tcgctttcgg tatacaggcg aaacccgcct tcagtacgca cttcatgctc catcatctgc
    10141 tgtttttcgt aataacgaat cgtgtcaggt gttacttccg ccatttttgc cagctcacca
    10201 atgcgataca tacatactcc actagttatc gttgattttg tccaacaact tgtcagcata
    10261 atcgccgcgt aaaaaatcgg tactcaatcc agcttgtcgc agcttcaatt ccagtaatga
    10321 caatcgacgg ctaacctctt gatattgcgg atcatcgtga cggatacctt tgagaatatc
    10381 cagaagctga attgcttctc tccgttgctc aagttctggc ggtaagcaac cggcattctt
    10441 cagcaagcga taccccgcac gtaattccgg tggcacgtga gaatcatcat ccagtatcaa
    10501 tggttcgccg ctacctgcca ggttatcaaa ctcacctttc gcttgcgctt ctgctatatg
    10561 gcgctctgcc cactggtcaa gtaaccacat aaatactcca ggggatgaac aaaaagagta
    10621 cagctattgt agataagtgg ggatactacg ggtataaaaa aaacccgccg gggcgggttt
    10681 ttttacattg cttcagatta ctctgcagca gcttctgctt tctctgaacg atcaaccagc
    10741 tcgatgtaag ccatcggcgc gttgtcgcct gcacggaagc cacacttcag aatacgagtg
    10801 taaccaccgg cacggctcgc gaaacgcggg cccagttcgt taaacagttt tgccacgatc
    10861 tcgttatcac gagtacgggc aaatgccaga cgacgattag caacgctatc agtcttggca
    10921 agagtaatca gcggctcaac tacgcggcgc agctctttcg ctttaggcag agtcgtcttg
    10981 atgatttcat gacgaaccag tgaacctgcc atattgcgga acatagcctg gcgatggctg
    11041 ctgttgcggt tcagttgacg accactctta cgatggcgca tgaccttatc cttctcagta
    11101 aaaccttaac ctgtgatccg gttactcgtc agcgatgctt gccggtggcc agttttccag
    11161 gcgcatgccc agagacagtc cacgggaagc cagcacgtct ttaatctcag taagagattt
    11221 tttaccaagg ttaggcgttt taaggagctc aacctcggta cgctgtacca gatcaccgat
    11281 atagtggata gcttctgctt taaggcagtt agcagagcgg acagtcaatt ccagatcgtc
    11341 aacagggcgc agcaggatcg gatcgaactc tggtttctct tctttcactt caggctgacg
    11401 tacatcacgt aagtcaacga aagcttccag ttgttcagcc agaatggttg ccgcacgacg
    11461 aatcgcctct tcaggatcga ttgtgccgtt ggtttccatt tcgatgacca gcttgtccag
    11521 gtcggtacgc tgttctacac gcgctgcttc aacattgtag gcaatacgct ccacagggct
    11581 gtagcatgcg tcgaccagca gacggccgat tgggcgctca tcttcttccg aatgaattcg
    11641 ggtagaagcc ggcacataac cacgaccgcg ctgaactttg atacgcatgc taatagacgc
    11701 gttctcatcg gtcaggtggc agatcacgtg ctgcggcttg acgatttcga catcaccgtc
    11761 gtgggtgata tcggctgcag tcacagggcc aatgccagat ttattcaagg taagaataac
    11821 ttcatctttg ccctgaactc tcaccgccag ccctttcagg ttgagcagga tttccaggat
    11881 atcttcctga acgccttctt tggtgctgta ctcatgtagt acaccatcaa tctcaacctc
    11941 ggtcaccgcg caacccggca tcgatgagag cagaatacgg cgcagtgcgt tacccagagt
    12001 atggccaaag ccacgctcta aaggctcaag ggtcaccttg gcgtgcgtcg aactcacttg
    12061 ctcgatatca accaggcgcg gttttagaaa ctctgtcaca gaaccctgca ttgtgtcctc
    12121 tctttggtac taagctttac ttggagtaaa gctcgacgat caggtgttcg ttaatgtccg
    12181 cagacagatc agaacgctcc ggcttacgct taaacgtacc ttccatcttg ccagcatcaa
    12241 cttccagcca ggttggcttt tcacgctgct cagccagctc cagagcggct ttcacgcgag
    12301 actgcttctt cgctttctca cgaatgctta caacgtcatt cggactaacc tgataagaag
    12361 cgatgttaac aacacgaccg tttaccataa ttgctttatg gctaaccagc tgacgtgctt
    12421 ctgcacgagt ggcaccgaag cccatacggt atacaacgtt gtccagacga ccttccagca
    12481 gagccaacag gttttcaccg gtgttgcctt tcagacgtgc tgcttctttg tagtagttac
    12541 ggaactgacg ctccagcaca ccatagatac ggcgaacttt ttgcttttca cgcaactgca
    12601 caccatagtc agacagacgc ggtttacgcg caccgtgctg gccaggagct tgttcaattt
    12661 tacacttggt atcgatcgcg cgaacgccag acttaaggaa taagtcggtg ccctcacgac
    12721 ggctcagctt gagcttagga cccaaatatc ttgccatttt ctttctccaa caaacctgga
    12781 aaacgaggcg ttatacgcga cgttttttcg gcggacgaca accgttatga gggatcggag
    12841 tcacatcagt aatgttagtg atgcggaaac ctgcggcgtt cagagcacga atagtagatt
    12901 cgcggcctgg acccggacct ttaaccataa cttccagatt cttgatgccg tattctttca
    12961 cggcgtcagc gcaacgctct gctgcaacct gagctgcaaa cggagtggat ttgcgagaac
    13021 cacggaaacc ggaaccaccg gctgttgccc aacccaacgc gttaccctga cgatcagtga
    13081 tagtcacgat ggtgttgttg aaagaagcat ggatatgagc cacgccgtca gagacttgtt
    13141 ttcttacacg tttacgtgca cgaattggtg cctttgccat tattcaatca ccccgattat
    13201 ttcttgatcg gtttgcgcgg acccttacgg gtacgtgcgt tggtcttggt acgctgaccg
    13261 cgaaccggga gaccacgacg atgacgcaaa ccgcgatagc aaccaagatc catcaggcgc
    13321 ttgatgctca tgctgatttc acggcgcaga tcaccttcaa cgacaaattt ggcaacttcg
    13381 tcacgcagcg tgtcgatttg tccttcagac agctcactga tcttaacatc ttcagcgata
    13441 cccgctgcag ccaggatggc tttagaacgg gtcttgccga cgccataaat cgaagttaat
    13501 gcgattacgg catgcttatg atcaggaatg ttaatgcctg ctatacgggc cactatgcac
    13561 tcctactatt taatatgtac gttccatgct gaaaagcccg ttttcaggat actcaaatgg
    13621 aaacgcacag acatacaaaa gattggctga ctaatctagc cagctcaacc caactttgca
    13681 agaaaaatat gcgaaaaaat cagccttggc gctgtttatg cttcggctcg gcactgcaaa
    13741 tcacacggat gacaccatca cgcttaacga ttttgcagtt acggcataat ttcttgacgg
    13801 aagcacgaac tttcattttt actctccgta acttctcggg cgaccaatta tcggccgtag
    13861 cctttcaggt tcgccttctt caatgcagac tcatactgac tggacatcat cagagtttgc
    13921 acttgagcca taaagtccat aatcacgaca acaacgataa gcagtgaggt cccaccgaag
    13981 tagaacggta ctttcattgc atcacgcatg aactccggga tcaggcagat aaaggtaata
    14041 tacagcgcac caaccagggt caggcgggtc attactttat cgatatactt cgccgtttgc
    14101 tctcccggac gaattcctgg tacaaatgca ccggacttct tcaggttatc tgctgtttca
    14161 cgcgggttga aaaccaacgc cgtgtagaag aaacagaaga agatgattgc agacgcatag
    14221 agtaacacat aaagcggttg cccaggctgc aaatacagcg aaattgttgt cagccagttc
    14281 caaccagtac cgcccccgaa ccatgacgcg atggtcgccg ggaacagaat aatactggaa
    14341 gcgaagattg ccgggattac ccccgccata ttcactttca gcggtaaatg tgtgctctgt
    14401 gcagcataaa cacgacgacc ttgctgacgt ttcgcgtagt ttaccacaat gcggcgttga
    14461 ccacgctcaa caaatacaac aaagaacgtc actgcaaata ctaatactgc aaccaacagc
    14521 aacacgagga agtgcaggtc gccttgacgc gcttgctcga tagtatgggc aatggctggc
    14581 gggagtcccg cgacaatacc gacgaagata atgattgaaa taccgttgcc gatacctcgt
    14641 tcagtaatct gttcgcccaa ccacatcagg aacatcgttc ctgtgaccag acttacaaca
    14701 gcggtgaagt agaatgcaaa gcccgggtta atcaccaggc cttgcatacc aggcatattc
    14761 ggcagaccag tagcaatacc gatcgactgg aatattgcca gcaccagagt accgtagcgg
    14821 gtgtactggc tgatcttacg acgaccagac tccccttctt tcttaatttc cgccaacgtt
    14881 gggtgaacca ccgtcagcag ctggataatg atcgacgccg aaatatacgg catgatcccc
    14941 agagcaaaga tagaagcacg gctgagagca ccaccagaga acatgttaaa catctcaatg
    15001 atggtgcctc gctgttgctc aagcagtttg gcaagtacag cggcatcaat accagggatc
    15061 ggaataaaag agccaatacg gaacacaatc agcgcaccga taacaaacag cagtctgcgt
    15121 ttcagctcgc ctaagccacc tttggcactt tgaaaatcta atcccggttg tttagccatc
    15181 tgctacttat tcctcgattt taccgccagc agcttcgata gcagcacgag cgcctttagt
    15241 aacacgcagg ccacgaacag ttaccggagt cgttacttcg ccagccagga tcactttcgc
    15301 gaactcgatc tggataccga taatgttagc cgctttcagc gtgttcaggt ctacaacacc
    15361 gccttctact ttagccaggt cagacagacg aacttcggct gtaatcgctg ctttacgaga
    15421 agtgaagccg aatttcggca gacgacggta cagaggcatc tgaccaccct cgaaaccgcg
    15481 acgtacgcca ccgccagaac gagacttctg acctttgtga ccacgaccac cggttttacc
    15541 gaggccagaa ccgataccac gacccaggcg tttacccgcc tttttggagc cttcggccgg
    15601 agacagagta tttaaacgca tctcttactc ctcaacttta accatgaagg aaaccgcgtt
    15661 gatcataccg cgaatagcag gagtatcctc gcgctctacg gtgtgaccaa tacgacgcag
    15721 acccaggcca agcagcgttg ccttgtgttt cggcagacga ccgattgcac tgcgggtttg
    15781 agtaatttta atagtctttg ccatggttta tttccccaga atttcttcaa cggatttacc
    15841 acgcttggca gcgaccattt ctggagaatt catattttcc aggccatcaa tagttgcacg
    15901 aaccacgttg atcgggttgg tggaaccata tgctttagcc agaacgttat gaaccccagc
    15961 gacttccaga acggcgcgca ttgcaccacc ggcgatgata ccggtacctt cggaagccgg
    16021 ctgcatgaat acgcgagaac ccgtgtgaac acctttaaca gggtgttgca gagtgccgtt
    16081 attcagcgcg acgttaatca tattgcgacg ggctttttcc atcgctttct ggatcgctgc
    16141 tggaacttca cgcgctttac cgtaaccaaa accaacgcga ccgttaccat cgccaactac
    16201 agtcagagct gtgaaggaga aaatacgacc acctttaacg gttttagata cgcggtttac
    16261 cgcgatcagc ttttcctgca gttcgccagc ttgtttttcg atgtgagcca tcttacacct
    16321 ctaccttaga actgaaggcc agcttcacgg gcagcatctg ccagtgcctg gacacgacca
    16381 tgatattgga acccggaacg gtcaaaggat acatctttga tgcctttttc cagagcgcgt
    16441 tcagcgacag ctttacccac agctgcagcc gcgtctttgt taccggtgta cttcagttgt
    16501 tcagcgatag ctttttctac agtagaagca gctaccagaa cttcagaacc gttcggtgca
    16561 attacctgtg cgtaaatgtg acgcggggta cgatgtacca ccaggcgagt tgcgcccagc
    16621 tcctggagct tgcggcgtgc gcgggtcgca cgacggatac gagcaaattt cttatccata
    16681 gtgttacctt acttcttctt agcctctttg gtacgcacga cttcgtcggc gtaacgaaca
    16741 cccttgcctt tataaggctc aggacgacgg taggcgcgca gatccgctgc aacctggccg
    16801 atcacctgct tatcaacgcc tttcagcacg atttcagtct gagtcggaca ttcagcagtg
    16861 atacccgcag gcagctgatg gtcaacagga tgagagaaac ccagagacag gttaatcaca
    16921 ttgcctttaa ccgctgcacg gtaacctaca ccaaccagct gcagcttctt agtgaagcct
    16981 tcggtaacac cgataaccat tgagttcagc agggcacgcg cggtaccagc ctgtgcccaa
    17041 ccgtctgcgt aaccatcacg cggaccgaag gtcagggtat tatctgcatg tttaacttca
    17101 acagcatcgt tgagagtacg agtcagctcg ccgtttttac ctttgatcgt aataacctga
    17161 ccgttgattt ttacgtcaac gccggcagga acaacgaccg gtgctttagc aacacgagac
    17221 attttttcct ccgattaggc tacgtagcag ataatttcgc caccaagacc agcctggcgc
    17281 gctgcacgat cagtcataac acctttagag gtagaaacaa ctgcgatacc cagacccgcc
    17341 ataactttcg gcagctcatc tttacgttta tagatgcgca gacctgggcg gctgacacgc
    17401 tgaatgcttt ctacaacagc tttgccctgg aaatacttca gagtaagttc cagttcaggc
    17461 ttggtgtcgc cttcaacttt aaaatcttca ataaaacctt cttccttcag cacgttggcg
    17521 attgccactt tcagcttgga ggaaggcatg gtgaccgcag ctttgttcgc ggcctgaccg
    17581 ttacggatac gggtcagcat atccgcgatc ggatcttgca tgctcatctg tctttactcc
    17641 cgtgattcaa ttggtgacaa ttaccagcta gcctttttca gacccgggat ttcaccgcgc
    17701 atagcggctt cacggacctt aatacggctc aacccgaact tccgcaggaa accatgcggg
    17761 cgacctgttt gacggcagcg gttacgctga cgagacgggc tggaatcacg cggcagagtc
    17821 tgcagcttga gaacagcgtt ccaacgatct tcgtcggaag cgttcacatc agagatgatc
    17881 gctttcagtt cagcgcgttt cgcgaagtat ttatcagcta aagctacgcg ttttacttcg
    17941 cgtgctttca ttgattgctt agccatttag taaccctacc ttacttgcgg aacgggaagt
    18001 caaaggcagc cagcagagcg cggccttctt cgtcagattt cgcagtagtg gtaatggtaa
    18061 tatccaaacc acgaacgcgg tcgactttat cgtagtcgat ttctgggaag atgatctgct
    18121 cacggacacc catgctgtag ttaccacgac cgtcgaaaga cttagcggac aggccacgga
    18181 agtcacgaat acgaggtaca gcaatagtga tcaggcgctc aaagaactcc cacatgcgtt
    18241 cgccacgcag agttacttta cagccgatcg gatagccctg acggattttg aagcctgcaa
    18301 cagatttgcg tgctttggtg atcagcggtt tttgaccgga gattgctgcc aggtctgctg
    18361 ctgcgttatc cagcagtttt ttgtcagcga tcgcttcacc aacacccatg ttcagggtga
    18421 tcttctcgac ccgagggact tgcatgacag aattgtagtt aaactcagtc atgagttttt
    18481 taactacttc gtctttgtag taatcatgca gtttcgccat cgtactactc caaattactt
    18541 gatagtttcg ctgttagact tgaagaaacg gacttttttg ccgtcttcga atctaaagcc
    18601 tacacggtca gccttgccgg ttgccgcatt gaagattgct acgttggaaa cctgaatagc
    18661 ggcttctttt tcaacgatgc cacccggttg gttcagggcc ggaaccggct tctgatgttt
    18721 cttaaccagg ttgatacctt caacaatgac cttgccggaa gacaggacat tcttaacttt
    18781 accgcgttta cctttatctt taccggttaa cacgataact tcgtcatcac gacggatttt
    18841 cgctgccatg attcgctcct tagagtactt ctggtgccag agagataatt ttcatgaact
    18901 tctcactacg aagctcacga gttaccggcc caaaaatacg cgtaccgata ggctgctcgc
    18961 tgttgttgtt cagaagaaca caagcattac catcgaagcg aatgacagaa ccgtccgggc
    19021 gacgaacacc cttcttggtg cgcaccacta ccgccttcag cacatcacct tttttgacct
    19081 taccacgcgg aattgcttct ttgatggtga tcttgatgat gtcgcctacg cctgcgtagc
    19141 gacggtgcga gccacccaga accttgatac acattacgcg acgtgcaccg gagttgtcgg
    19201 cgacgttcag catagtctgt tcttggatca ttttagtgct ccgctaatgt caactactac
    19261 tgagacccga aaatcaggtc gttaaaaatc cccatatcga gggcgcggca ttataacacc
    19321 gcttcaagga tatgggtaga aaaaataaac ggctcatttc tgagccgttt attcgtatcg
    19381 agagagtgta ctgtattaca gaaccgcttt ctctacaacg cgaaccagcg tccaggattt
    19441 agtcttggac agcggacggc attcgcggat ttcaaccacg tcaccgatac cgcattcgtt
    19501 gttctcgtca tgtacgtgca gtttggtcgt acgcttgatg aatttaccgt agatcgggtg
    19561 tttcacaaaa cgttcgatag caacaacaat ggatttctcc attttgtcgc taacaacgcg
    19621 accttgcaga gtacggattt tatcggtcat tacgcacccg ccttctcgtt cagtaaagtc
    19681 ttaacgcgtg cgacatcgcg acgcacttgc ttcaacaggt gagactgttg cagctggcca
    19741 cttgcagcct gcatacgcag gttgaactgc tcacgcagca ggttcagcag ctcggtgttc
    19801 agctcttcaa cgctcttctc acgcagctct tttgctttca ttacatcacc gtcttagtta
    19861 caaaggtggt tttaatcggc agtttcgctg ctgccagctt gaatgcttca cgggccagct
    19921 cttccggaac accgtccatt tcatacagga ctttacccgg ctgaatcaag gcaacccaat
    19981 actccacgtt acctttacct ttacccatac gcactgccag cggcttttca gtgatcggtt
    20041 tgtccgggaa cacacggatc cagatcttac cttgacgctt aactgcacgg gtcatagcac
    20101 gacgtgctgc ttcgatctga cgggcagtca gacgaccacg gccaacagct ttcagaccga
    20161 agctgccgaa gctaacatcc gtaccctgcg ccagaccgcg gttacggcct ttgtgcattt
    20221 tacggaattt tgtacgcttt ggttgtaaca tcagcgacgc tccttattta cggcctttac
    20281 gctgctgctt tttaggctga gcagccggtt tttccggttg ttcaacagca gccataccac
    20341 ccaggatctc gcctttgaag atccacactt taacgccgat tacaccgtaa gtggtgtgcg
    20401 cttcagaggt gttgtagtcg atgtcagcac gcagagtgtg cagcggtacg cgaccttcgc
    20461 ggtaccattc ggtacgtgcg atttccgcgc cgcccagacg gccgctaact tcaactttaa
    20521 tacctttagc gcccagacgc attgcgttct gtacagcacg cttcatagca cgacggaaca
    20581 taacgcgacg ttccagctga gaagtgatgc tgtcagcaac cagttttgcg tccagttcag
    20641 gcttacgaac ttcggcgatg ttgatctgtg caggaacgcc agcgatgtcc gctacgacct
    20701 tacgcagttt ttctacgtct tcaccttttt taccgataac gatacccggg cgagcagtgt
    20761 gaatggttac acggatgctc ttagccggac gctcgataac gatacgagat acagacgctt
    20821 tagccagttc cttagtcagg tactgacgta ctttaaaatc gctgtccagg ttgtcagcga
    20881 attctttggt gttcgcaaac caggtagagt tccatggttt tacaataccc aagcgaatac
    20941 cattaggatg tactttctga cccattgcta gtctccagag tctcagcgat cggacacaac
    21001 cacagtgatg tggctggtgc gcttcaggat gcgatctgca cgaccttttg cacgcggcat
    21061 aatgcgcttc atgctcgggc cttcgtctac gaaaattttc gtaactttca gatcgtcaat
    21121 gtcagcgcca tcgttgtgtt cagcgttagc aatggcagat tccagaactt tcttgaccag
    21181 tacagccgct ttcttgttgg tgtaggtcaa aatatccaga gcctgcgaca ctttcttacc
    21241 gcgaatcagg tcagcaacaa ggcgaacctt ctgagcagaa gaacgagcat ggcgatgttt
    21301 agcgatagtt tccatctctt cctcctacct tatttcttct tcgctttttt atcagcagcg
    21361 tggccgcgat aagtacgagt cggtgcgaat tcacccagtt tgtgaccgac catttcgtcg
    21421 gtcacaaata ccggaacgtg ctgacgacca ttatggacag cgatggtcaa accgatcatg
    21481 ttaggaaaga tcgttgaacg acgggaccaa gtgcgcaggg gcttcttgtc tccgctttcc
    21541 accgctttct ctaccttctt cagcaagtgc aggtcaataa aaggaccttt cttgagagaa
    21601 cgtggcatgg cttatcctct aaaattattt gctacggcga cgtacgatga atttatcagt
    21661 acgcttgttg ctgcgggtct tcttaccttt ggtctgaacg ccccacggag ttaccgggtg
    21721 cttaccaaag ttacgacctt caccaccacc atgtgggtgg tctaccgggt tcatcgcggt
    21781 accgcgaacg gtcggacgaa caccacgcca gcgtgcagca cctgctttac ccagaacgcg
    21841 cagcatatgc tcagcattgc caacttcgcc cagagttgca cggcagtctg cttctacttt
    21901 acgcatttca ccagaacgca gacgcagggt gacataagca ccatcacgag caacgatctg
    21961 aacgtaagta ccagcggaac gtgccagctg accgccttta cctggtttca tttctacgtt
    22021 atgaacagta gaaccaaccg ggatgttgcg catcggcagg gtgttacctg gtttgattgc
    22081 agcatcaacg ccagactgaa tctggtcgcc agctttcagg cctttagggg ccaggatgta
    22141 acggcgttca ccgtctttgt acagaaccag cgcgatgttc gcggaacggt tcggatcgta
    22201 ctcaagacgt tcaacaactg ccgggatacc gtctttgttg cgtttgaagt caacaatacg
    22261 gtaagcctgc ttgtggccac caccgatatg acgagtggtg atacggccat tgttgttacg
    22321 accaccggat ttgctgtttt tttccagcaa cggagcaaaa ggtttgccct tgtgcagctc
    22381 agggttaacc actttaacta cgtggcgacg acccggagat gtcggtttac atttaacaac
    22441 tgccattgta ttactcctcc gacttactca gcgccgccaa cgaagtccag attctggcct
    22501 tctttcaggg tgacgtaagc ttttttccag tcgctacgac gaccgatacg ctgtccgtga
    22561 cgtttaactt tccctttaac taccagggtg ttaacgactt cgacttcgac ttcaaacagt
    22621 ttctgcacag cagctttgat ttctgctttg gtcgcgtctt tagcaacttt gagtacgatg
    22681 gtgttggatt tttccatcgc agtagacgct ttttcagaaa cgtgcggtgc acgcagcacc
    22741 ttcagcagac gttcttcacg aatcatgcca gcatctcctc aacttgctta acagcatcag
    22801 cagtcattac gactttgtcg aaggcgatca ggctaaccgg gtcgatacca gttgcatcgc
    22861 gtacgtcaac cttgtgcagg ttgcgcgcag ccaggaacag gttttcgtcc agctcaccgg
    22921 tgatgatcag cacatcttcc agagccatgt ctttcagttt ctgtgccagc agcttagttt
    22981 tcggcgcttc tacagagaac ttctcgacaa cgatcagacg atcctgacgt accagttcgg
    23041 acaggatgct tttcagcgcg ccgcggtaca tcttcttgtt aactttttga ctgtggtcct
    23101 gcggacgagc agcaaaggtc acgccaccag aacgccagat cgggctcttg atagaaccag
    23161 aacgcgcacg gccggtgcct ttctggcgcc acggtttttt accggaacca gttacttcag
    23221 cacgagtctt ctgagcacga gtaccctgac gagcaccagc tgcataagca acaacaacct
    23281 ggtgaaccag cgcttcgttg aaatcacgac cgaaggtagt ttcggaaaca gtcagcgcgc
    23341 tctgcgcgtc tttcaatact aattccattg ctatctcctt acgccttcac agctggttta
    23401 acgatcaggt cgctaccggt tgcacccggg acagcacctt taaccagcag caggttgcgc
    23461 tcagcgtcaa cgcgtactac gtcaaggctc tgaacggtta cacgttcgtt acccatctga
    23521 cctgccattt tcttgccttt gaacactttg cccggagtct ggttctgacc gatagaaccc
    23581 ggaacgcggt gagacaagga gttaccgtga gtagcgtcct gggtacggaa gttccagcgc
    23641 ttaacggtac ctgcgaaacc tttaccttta gaggtgccag ttacgtcaac ttttttaacc
    23701 tcagcaaaca gttcaacgct aatgctctga cctacagtga actcttcgcc ttcagccagg
    23761 cggaattccc acagaccacg gccagcttct acgccagctt tagcgaagtg gccagcttca
    23821 ggcttggtca cacggttagc ttttttagca ccggtggtca cctgaatagc acggtagcca
    23881 tcgttagcca ggtctttaac ctgagtaacg cggtttgctt caacttcgat tacggttact
    23941 gggatagaaa cgccgtcttc tgtgaagata cgggtcatac ccactttttt accgactaaa
    24001 ccaatcattg tttcaacctc tcaatcgctc aatgacctga ttaacccagg ctgatctgca
    24061 cgtctacacc ggcagccaga tccagacgca tcagagcatc aacggttttc tcggttggct
    24121 caacgatgtc aaccagacgc aagtgagtac ggatttcgta ctgatcgcgc gcgtctttgt
    24181 tgacgtgcgg ggagatcaga acagtgaagc gctctttgcg tgtcggcagc gggatcggac
    24241 cacggacctg cgcaccagtg cgcttggcag tctcgacgat ttccgcggtt gcttgatcga
    24301 tcagacgatg atcaaacgct ttcaggcgga tacggattct ttggttctgc atgagaccag
    24361 agctccaatt attttataaa cgaaaatgat tactcctcag acccattacg attgatggga
    24421 gagtgtaacc gttcttacgt agctccccga ttgggagcat tgttaggtag ccaaattcgg
    24481 ctaactgagg ttcagattga acctgctgtc aactacgaca agcccgcgca ttatacatac
    24541 ttaaccaccg aacgcaagcg tattgctagt aaagtaaaca gccttcgggt tatctctcaa
    24601 gttcgttcag cgcatttttg acacgctctc ttaaaccggc tttctcgtcg ctttctttta
    24661 cagtattttc ttcgtcttca gaagaagagg gagcattctc aaccgcgacg tctgggtcat
    24721 cttcctcctc gacggattcg acgacgggga gtggctgttt tttgaagaaa tagtgttgtg
    24781 ttgattgttt aaaagcgggc gtttcagcct ctacagtgac ttctgctttt actatccaaa
    24841 gcgcccatag ctgcctggca tacccaccaa ccaacaccat tcctgcaaac cacaggcaaa
    24901 tcagcaataa acaaatgaca tatattatgg tgctgtgttt ttgtcgggag aagataccag
    24961 ggtgacctgt ttctttctgc tcacgcgcgg cgatatagaa ttcaaacatg tcatttcccc
    25021 ttcactgttg tttccgggga aatatttgct tcatcccgat ataagtaggc actctcccct
    25081 gccacctgcc agagatgtac taacgttgaa aagccaacaa caccgtcagt tttcaagtgg
    25141 tggctttttt gaaattgctt tattttttct accaacaagg gacgccactc cgccgaaggt
    25201 tctgtcgaaa tatgcaatgc acggtttaac atcgtttcca gccagagaat ctcttcctcg
    25261 ctgctatcac gcgtaatggt actctcccct tccggcgaca ttttccacaa cagaagataa
    25321 tcacccgtcc ataccgactc aaaccattta tgtgtcagcg tccacgtttg ctgacctacg
    25381 aggacatcaa cgctagcctc accgacgcga acgaccacaa caggcaattt tttgtccccg
    25441 actttaagcg atgcgctcca gggcaaattc tgatctacca atgtttgtaa agaagc
//
LOCUS       DAJVQG010000048        25333 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJVQG010000048 DAJVQG010000000
VERSION     DAJVQG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25333
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.048"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..1254)
                     /locus_tag="OQM83_001777"
     CDS             complement(<1..1254)
                     /locus_tag="OQM83_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCS1520059.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPR"
     gene            complement(1257..2924)
                     /locus_tag="OQM83_001778"
     CDS             complement(1257..2924)
                     /locus_tag="OQM83_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1520060.1"
                     /translation="MSATRIQAVYRDTGGEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(2921..5029)
                     /locus_tag="OQM83_001779"
     CDS             complement(2921..5029)
                     /locus_tag="OQM83_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCS1520061.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(5016..5837)
                     /locus_tag="OQM83_001780"
     CDS             complement(5016..5837)
                     /locus_tag="OQM83_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCS1520062.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(5997..7826)
                     /gene="glmS"
                     /locus_tag="OQM83_001781"
     CDS             complement(5997..7826)
                     /gene="glmS"
                     /locus_tag="OQM83_001781"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCS1520063.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(7988..9358)
                     /gene="glmU"
                     /locus_tag="OQM83_001782"
     CDS             complement(7988..9358)
                     /gene="glmU"
                     /locus_tag="OQM83_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCS1520064.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(9709..10128)
                     /gene="atpC"
                     /locus_tag="OQM83_001783"
     CDS             complement(9709..10128)
                     /gene="atpC"
                     /locus_tag="OQM83_001783"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCS1520065.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(10149..11531)
                     /gene="atpD"
                     /locus_tag="OQM83_001784"
     CDS             complement(10149..11531)
                     /gene="atpD"
                     /locus_tag="OQM83_001784"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCS1520066.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(11558..12421)
                     /gene="atpG"
                     /locus_tag="OQM83_001785"
     CDS             complement(11558..12421)
                     /gene="atpG"
                     /locus_tag="OQM83_001785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCS1520067.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKSVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(12472..14013)
                     /gene="atpA"
                     /locus_tag="OQM83_001786"
     CDS             complement(12472..14013)
                     /gene="atpA"
                     /locus_tag="OQM83_001786"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCS1520068.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(14026..14559)
                     /gene="atpH"
                     /locus_tag="OQM83_001787"
     CDS             complement(14026..14559)
                     /gene="atpH"
                     /locus_tag="OQM83_001787"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCS1520069.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(14574..15044)
                     /gene="atpF"
                     /locus_tag="OQM83_001788"
     CDS             complement(14574..15044)
                     /gene="atpF"
                     /locus_tag="OQM83_001788"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCS1520070.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(15106..15345)
                     /gene="atpE"
                     /locus_tag="OQM83_001789"
     CDS             complement(15106..15345)
                     /gene="atpE"
                     /locus_tag="OQM83_001789"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCS1520071.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(15392..16207)
                     /gene="atpB"
                     /locus_tag="OQM83_001790"
     CDS             complement(15392..16207)
                     /gene="atpB"
                     /locus_tag="OQM83_001790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCS1520072.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(16216..16596)
                     /gene="atpI"
                     /locus_tag="OQM83_001791"
     CDS             complement(16216..16596)
                     /gene="atpI"
                     /locus_tag="OQM83_001791"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCS1520073.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(17213..17836)
                     /gene="rsmG"
                     /locus_tag="OQM83_001792"
     CDS             complement(17213..17836)
                     /gene="rsmG"
                     /locus_tag="OQM83_001792"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCS1520074.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(17900..19789)
                     /gene="mnmG"
                     /locus_tag="OQM83_001793"
     CDS             complement(17900..19789)
                     /gene="mnmG"
                     /locus_tag="OQM83_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCS1520075.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(20168..20611)
                     /gene="mioC"
                     /locus_tag="OQM83_001794"
     CDS             complement(20168..20611)
                     /gene="mioC"
                     /locus_tag="OQM83_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCS1520076.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(20701..21159)
                     /gene="asnC"
                     /locus_tag="OQM83_001795"
     CDS             complement(20701..21159)
                     /gene="asnC"
                     /locus_tag="OQM83_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCS1520077.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            21311..22303
                     /gene="asnA"
                     /locus_tag="OQM83_001796"
     CDS             21311..22303
                     /gene="asnA"
                     /locus_tag="OQM83_001796"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCS1520078.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(22308..23759)
                     /gene="viaA"
                     /locus_tag="OQM83_001797"
     CDS             complement(22308..23759)
                     /gene="viaA"
                     /locus_tag="OQM83_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCS1520079.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(23753..25249)
                     /gene="ravA"
                     /locus_tag="OQM83_001798"
     CDS             complement(23753..25249)
                     /gene="ravA"
                     /locus_tag="OQM83_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCS1520080.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRLQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
BASE COUNT         6279 a         6567 c         6002 g         6485 t
ORIGIN      
        1 tcttgggtga tcaaggctac tatctagacg ctttatgatt cttaatagtt gtcgtacagc
       61 aattcgatct ctttggttcc aatcaactct aggggcgggt gccagacgct cttgttgatg
      121 ctgttgattc cagtgaacta gccaatccct gtcatgtcgg taaagccaag cgtatagcac
      181 cccaccctct aaagactgtc ttgccgcctt aattccttgg tatttatgca ctagttgctg
      241 ccagtcttta tgcttaacag ataaatcttc agagttaggt tgcacagact ggctaacagg
      301 tcttgtcgtt atagagtgct cagtaagagc acttgcctgc tgtagcgctt ctataaccgt
      361 tagttttggc aataaggctt gccacacaat actatgctgt aaataactaa aggcttttct
      421 atgcttacgg aatatacttt tcagccaaca cgtgtccttg ttctctgcca actttaaatc
      481 cagtttctct agtgcctcat cactaaaagt ctgcctcact ctctccgcca ccaagtcatg
      541 acgaatgtgc ttgcttttgg ttagccctag atcctgcgct aagcgctgat aaaacagcgt
      601 ccactgctca aggcttgggg aaagctcttg cgctcgtgga gcatctaaca gaggggctat
      661 ataagcagct agtgctgtta attgagatag ggagtctttg gggtagtctg aaagcagctc
      721 agtatgaccc aaagcccaaa attgatgtcg gtgatcatct acagctctat caaagaagac
      781 taaagcaccg tgttttggac aatatggcaa agcgggcaaa taccaatctc gttgccaaaa
      841 ggcttcccca tacctattta gctgaagagc aacgcaatca gggcagtatc taaagcggtt
      901 atcgctctta actctagaag cagcgactcc tagcattaaa tgcaccgcac cttgcgcttg
      961 gtactccatt aaccgaatag cttcgtctcg gcgctccttg cctacaaacg gagcatataa
     1021 agggaataag gtatgctcat aaataagctg ctgaacagcg taacgtcctg tttgatgtag
     1081 atgtcttgct atcacaccta aatgcgaggg cagacctaag gtagcgacca ccttgcggtt
     1141 gccatacacc tcatccaaca gctgcttagg actaacaatc ccttgataaa cgcctgcccg
     1201 tgcaatagtg ctataaatca gctcattcga gtacggaaca ggaaagtttc tcatggctaa
     1261 cctgcttgtt taaataagct cgccatatcc actattaccc ctttcccttt taaccgttca
     1321 tgcatggttt tttcaggttg gcgttgtgaa tagatataac gtaaatccgt atcaggcaag
     1381 ctatcccagt cgctgggctt gactaccttt atagccgaaa cctttttact cttggagggc
     1441 ttttctagtt ctgacactac cgtttcgcct tccatcaacc actgcaaaac aagaggcagt
     1501 aacttttgtc ttgtcatcgt tggattctgg ctaaacgctt ttttaatagt gggaattaac
     1561 aggcttgaat cgtaatcctc tttcagcatc agatataaat gacgctgatc ttcggtatct
     1621 aactcttgaa gggctttttc ttctggtgtt tgttcttgta tcgctgcgat atctagctga
     1681 agttggatta accgtttatc aatctcggga acgactagat cagaataacg agcaatgcgt
     1741 tctgggatac ccgagcgtaa tgcctctagc atggggtgca caggctttaa ctcatcttga
     1801 tacacttgcc gcaataaacc agcggtaata cgctcattgc ctaaagctag cgcacggagc
     1861 tgagcgagta caaaaagttt tactacaatg tccatcactc cttggcttag ctcataccac
     1921 acatcacgga cctcatccga taacagcgca tctttgcgtt gtaaaagctg taattgccaa
     1981 agattatccg taaaagcgat ccactcttga ttgggctttc cacgttgcgt ttgttgtata
     2041 ggatcccaga atatagctcc aaaccctgcc cctctacgtg cagaccgcaa atcagcctca
     2101 aaaatctctc gtgctttagg ggtaccaatc aacatcactg gtacgccaat aatattcacc
     2161 atcgtcacaa aaaagttcag catctcttga gatccacccg aacgagagcg gcttaaatgc
     2221 tgaatttcat caataaccaa caaccctaaa gcatgtgcat tggctatttg cgacatcaaa
     2281 gccaacatgg tttctatacc atgacgtttt aagccataac gacgctcata gttcgagccc
     2341 aaggctcgat ccaacgctct gaaaaaattc aagcagattt cttttagcga accattatgc
     2401 gagcagtcta ttttcaaata caccacctgc tctacattga gttcacgatg gtaaatcacc
     2461 tgaggatacg tggctagaat acgatgaaga gaggtcgtct tcccactacc agaacaacca
     2521 attaacaata agctttgtgc cgtagatcgt gcctcctcaa agcgaaatgt ctccaactct
     2581 cccgtttgaa cacgctcata accattttgt aaatgctttt gtaaatctcc tgttttagga
     2641 tttctgccta cgtagccacc tcgaatcatg accgaaatac gctcacttag tagcaaatgc
     2701 gtacctaatg gctgaaaata gtcatctgga atacgacaaa tggtatgagc tctgataaca
     2761 cgggactttt gcaagtcaga ggaagtaagc tgtaaagagg atttcagtga tgcagcacta
     2821 ttcactgact cttgtaatgg tggtaaggcc tcgataaaag gattatcacg ataagcctct
     2881 ccccccgtat cacgataaac tgcttgaatc cgggtagcac tcatgactca tccttttctg
     2941 gtggatcctg aaataattca ggcacgtatg tgggtaggct gtaatcttct tgatcatccg
     3001 cttccactgc gttgaaagga ataactttag cctcatcacc tgaagagctt ggcttcaaat
     3061 gctccgcacg ttttttacgc tccgaggtca cggcttcttt tttattagtt ttaatctgct
     3121 taatgcgtgt tgatttgggc tcagtagtac tgggcgttaa cttattcgct ttctgaatgg
     3181 tttgctgaat aaacgcctca agctccctgc gtttagttaa ctcatcctgc ttcgcattgg
     3241 ctttattgtg tttttcttgt gcttgtatat cccaaacctc ccaaaatgag agacctttaa
     3301 actgccgact acgttccgtc agattacagc gccaaaatac acggctgcca acttgcggaa
     3361 acaaataaat cgtatcaacc agcactgggt cataagccgc ttctaaatgt tgaggtctag
     3421 ctatatcagt gctccgctgc aaccaaccct cacgcagaat ctctgacccc gagtaataca
     3481 aaccccacaa attaacgcca aatgaagaaa tagagacctt tcggcgaggc agtaacgcta
     3541 ctcgcaactg ctcttgctcc acagccctta aactacctgt acgatgctgc ataccccatt
     3601 gccatagctg gacaggaata gacggtaaat ctgtaggaaa atcagcatct cgatcgtatt
     3661 tatccatcac cagatggtta tttctgaata agatcgtacg caaaataatt tgtgtgaact
     3721 caaataccga cagagatgca tctaacctat agtctgtttc accatggctt ttgatccgac
     3781 tgccctctac aatgccaggt gcaaaggact taaactcggc ttgtagtgtt ctaaaagtgc
     3841 tttccactat gcctttagca tcgccacgtc taggtggagc actttccact cgcacattaa
     3901 aactagaaac taaggcttcg acctgatgac tcattaattc gccacggtcc gctagcaaca
     3961 catctggcaa acctacacac ggccagtctg agctactaat ctcaatatca tgctgggcac
     4021 aaatggccgt tttgtcagag caagcattta caaaagcctg catcgccacc acataagacg
     4081 gattttcaaa gccgatataa aagcccgtga tcatccgact aaacacatca atcacaatgt
     4141 aaagcgttgg tcttcctatg attttttggc gatcatgatg atccactaaa taaatatcag
     4201 caatcgtggc atcaatctca taacgactcc cagggcctaa cgcctgagaa gtggctgtac
     4261 tacttaaggg tcgtacgtct tttttatata cccctgcttt aactctagac tttaagcgct
     4321 gagctttagg gtattctcga tcataaaaat aacgaaactg acgtagtgtt gggtaatcct
     4381 cttggggaat gcgaggaaaa tactgagcaa acaagtccac aaatcgtcta taggcaacgg
     4441 tggtctttgt acctttttga tttaacaggt gcttttctat ggtcaaccta aaaaggcgtt
     4501 caatctcggg cgttaccttg gttccttcac ccttaccata ttctctggct cggccaatct
     4561 ttgctgttcc tgtcgctgaa cgtctttccc ctggtgcacc gctgtttttg tagtcaggaa
     4621 ttaatgcatt aggcgtttga ccacgctgcc agtaacggcg taacaactta taaactgtag
     4681 ccttagtaac cttatgttct tggaccacat gctcaacgag ttcgcttctg actttagggt
     4741 cgaaacgatc cttactatta ataataggaa gaatttttcg ataatcctcc tcgcgcttct
     4801 ggaagctgac agaatctaca gacggctctt ctaaatcaag atgtacataa gggtcatcag
     4861 ttctaactaa gcggccttca tctaaatact gcattagcaa caactcagcc ctagcttgtg
     4921 gaactccttt atcagcgctt atttgcatcc agacaacttg gccatcctct atagccaaaa
     4981 tgcgatacgg atcattatca aatagcacaa cctcattaat ttgccacata gcgcaactcc
     5041 tccatattca ctacttggct aatacagaga tctgcacact tatttgccct gaaagactta
     5101 taaatattga acttaataaa accatttgcg gttaaggctc gtatctcact caatgttttg
     5161 cctaactcca aatcataagc aatatcaacc tgcttacaga cattgataat gttttcatct
     5221 cctttttctt gcaggatatg ggccaatggg gatagttgtg ctaaaagctc cgcagaaact
     5281 tcttctgttt tcactgaata aagccattca atattttctt ttactacggg atttatttct
     5341 ttatcagtaa aaatgaacca aggaatttgc ttttgctgcc aatagcgacg ctctagttct
     5401 agtttttcta aggtacgctc gtcttgtaag gctgctgcag gtttgacttg aatagcaaac
     5461 tgctcaaaag gaccatcttt gcagtccact aaaaaatcag tagacataac ctgatctaca
     5521 ccacgaataa caggatgctt aataccacta tctattgcaa tctgcctggt atcactaggt
     5581 aataagggga actgctcgcg tatatctagc acgctgctct cccactcaag actgagaaaa
     5641 acagcaagct ctaagtcaga tagcaaatga tggactcgtc ccgtcttatg agaataaata
     5701 cggtgggaac gacctgaaga aggaacttct tgtactgtta gccatggaat atagtcttta
     5761 ccatgccctt ggccacgccc ctctttaata cgacgggcaa tttgcacttc agaaaatgaa
     5821 gagtttgctt tagccataac aaaagtccag tatgcttttt cacagcataa ctggactgat
     5881 ttcagtttac aactattctg tctagtttaa gactttattg tcatagttta gatctatttt
     5941 gttcagttta agactttatt gtccgcccac aagccctttt ttatctatga tagtgattac
     6001 tcaaccgtaa ccgattttgc caggttacgc ggctggtcaa cgtcggtgcc tttgatcagc
     6061 gcgacatggt aagccagcag ctgcagcgga acggtgtaga agatcggtgc aatcacctct
     6121 tccacatgcg gcatctcgat gatgtgcatg ttatcgctac ttacaaaacc cgcatcctga
     6181 tcggcgaaga catacaattg gccgccacgc gcgcgaactt cttcaatgtt ggatttcagt
     6241 ttttccagca attcgttgtt cggtgcaacg acgataaccg gcatatcggc atcaataagc
     6301 gccagcggac cgtgtttcag ttcaccagca gcgtaggctt cagcgtgaat gtaggagatc
     6361 tctttcagct tcaatgcgcc ttccagtgcg attgggtact gatcgccacg gcccaggaac
     6421 agtgcgtgat gtttgtcaga gaaatcttct gccagagctt caatgcgttt gtcctgagat
     6481 agcatctgct caatacggct cggcagcgcc tgcagaccat gcacaatgtc atgttcaatg
     6541 gaggcatcca gacctttcag gcgagacagc ttcgccacca gcatcaacag cacagttaac
     6601 tgagtggtga atgctttagt ggacgccacg ccgatttccg tacccgcgtt ggtcatcagc
     6661 gccagatcgg attcgcgcac cagagaagaa cccggaacgt tacagattgc cagcgaacca
     6721 aggtaaccca gctctttcga cagacgcagg cccgccaggg tatccgcggt ttcgccagac
     6781 tgtgacaagg tgatcatcag gctgttacga cgcacggcag atttacgata gcggaattca
     6841 gaggcgattt cgacgtcgca cggaatacct gccagcgatt caaaccagta gcgggaaacc
     6901 ataccggagt tataagaagt accacaggcg aggatctgaa tatgctcaac cttcgacagc
     6961 agttcgtcgg cgttcggtcc cagctcgctt aaatcaacct gaccgtggct gatgcgcccg
     7021 gtaagggtgt tttttatcgc gttcggctgt tcgtagatct ctttctgcat gtagtgacgg
     7081 taaatacctt tatcgcccgc gtcatattgc agattggatt cgatatcctg acgttttact
     7141 tccgcgccag ttttatcgaa gatgtttacc gaacggcgag tgatttccgc aatatcgccc
     7201 tcttcaagga agataaagcg acgggtcacc ggcaacagcg ccagctggtc agaagcgata
     7261 aagttttcgc ccatacccag gccaatcacc agcggactac cagaacgtgc cgccagcagg
     7321 gtatccgggt gacgggagtc catgatcact gtaccgtacg caccgcgcag ctgcgggata
     7381 gcacgcagaa cagcctcacg cagagtcccg ccttgtttca gctcccagtt caccagatgg
     7441 gcaatcactt cggtgtcggt ttcagaaacg aaggtatagc cacgcgcttt taactcttca
     7501 cgcagcggtt catggttttc gatgatgccg ttatgcacca ccacaatgtg ttcagaaaca
     7561 tgcggatgcg cgttagcttc tgaaggttca ccgtgggtcg cccagcgagt gtgagcaata
     7621 ccggtgccgc catccagagg atgttcttcc gctgcctgag ccagcatctg gactttaccg
     7681 aggcgacgca ggcgggtcat atgaccttcc gcatcaacaa cggccagacc ggcagagtca
     7741 tatccgcggt attccagacg acgtaaacct tcaagaagga tttctgctac atcacgttgc
     7801 gcgatcgcgc caacaattcc acacatagtt tttgattccg atttatatcg ttgtcggtca
     7861 acctgtatgc ccgttttttc gggcgccccg agccttgtag agagtggggt tatttttata
     7921 gttactgctt gtgggaggga gattatgtta tctcctcatc ccagatgacc ggattacccg
     7981 gccagaatta ctttttcttt accggacgac gccagccttc tttctgagtc tgcggcacac
     8041 ggctgatagc taatgcattt tcgccgacat tacgcgtcac agttgtaccc gcagcaatgg
     8101 tcgcgccttt gcctactgtt accggggcca ccagctgagt gtcggaaccg acaaacacat
     8161 cgtcgccgat aatggtctta aatttattcg caccatcgta gttgcaggta atggttcccg
     8221 cgccgatgtt aacgttatcg ccaatttccg catcgcccag gtaagtcaga tgcccgactt
     8281 tcgagccttt acccagacgc gcttttttca tctcaacgaa gttaccaacg tgtgcacctt
     8341 ccagcaactc agcaccagga cgcaaacgag caaacgggcc aatagtacag gccgctgcca
     8401 gattcgcgtc ttccacaacg gtatacggac tgatttcgca atcatcgcca atcacgctgt
     8461 ttttaatcac gcaaccggtg ccgattttca cgcgatgacc gagagtcacg ttgccctcga
     8521 tgataacgtt agtatcaatt tcaacatcgc gcccgtgagt aagcgtaccg cgcagatcaa
     8581 aacgcgccgg atcgcgcagc ataacgcctg ctaacagcag tttttcagcc tgttcggact
     8641 ggtaaacgcg ctccagacgg gagagttgca ggcggttatt cacaccttct acttcgctta
     8701 aacgttgcgg atgaacggcg acgatttcac gcccttcctg atacgccagc gcaataatgt
     8761 cggtgatgta gtattcgccc tgagcattat tgttggtcag cttcgccagc cagcgtttca
     8821 tatctgcgcc gttagcgatc agaatgccgg tgttgatctc ctgaatctga cgctgctcgt
     8881 cggtggcatc tttgtgctca acaatgccgg taactttgcc attttcacgg gtgatacgtc
     8941 cataaccggt cggatcatcc agtttcaccg tcagcagacc aatgccaccc tgcggtttag
     9001 catcacgcag gcgctggagt gtttcgacag agatcagcgg cacgtcgccg tagagcatta
     9061 aaatgtcttc atcatcggca aagaaaggtg cggcctgctg cattgcatga cccgtaccca
     9121 gctgctctgc ctgaagcacc cagttaaggt tgtcgtcttt cagcgcctgt ttaagcagat
     9181 cgccgccgtg accgtacacc aagtgaacgt gcgctgcgcc taattcattc gcagcatcaa
     9241 tgacatgctg aaccatcgct ttcccggcaa gggtatgcag cactttcgga agatcggaat
     9301 acatgcgcgt gcctttgcct gcggcaagga tcactacgct catagcatta ttcaacatac
     9361 gcgtcctgac tgtaatttga gaacgaattt aaaccgcttc accttgaaaa aactacattt
     9421 ttttcatcgt gaaatggaca gaggataaat tgttcaataa cggattatcc ccgctgacga
     9481 aagtgccatt ttcgaccatc gtcacgcctt ttgtctgcag aaaattaagg cagtaccttc
     9541 atttaagcgt caggtggatg tttttgctct tatttcgatc aatgaataaa cgggaaaaca
     9601 ggggtgtttt atatcagcag gatctatgtg aacgctcttc aggacggatc acgcgtaaaa
     9661 aaaagccagc ctgtttccag actggctttt gtgcttttca agccggtgtt acatcgcttt
     9721 tttggtcaac tcgataacgc gcagctgcgc gatcgctttg gccagttccg cagacgcctg
     9781 agcgtaatct acgtcgccgt gagagctgct aatgtgctct tcagccttac gtttcgcttc
     9841 catggctcgc gcttcgtcga gatcctggcc gcgaattgcg gtgtcggcca gaacggtcac
     9901 gttgccaggc tgcacttcaa gaatgccgcc agacagatag ataaactctt cgtgaccgtg
     9961 ctgtttcacg atgcgaatca taccaggctt aatggcggtg agcagcggtg cgtggccagg
    10021 gtagatcccc agttcacctt cgctacccgt tacctggatt ttctcgacca gaccagagaa
    10081 catttgttgc tctgcgctga cgacgtccag gtggtaagtc attgccatat caccctccga
    10141 ttaaggcgtt aaagtttttt ggctttttcc acagcttctt cgatggaacc gaccatgtag
    10201 aacgcctgct ccggcaggtg atcgtattcg ccttccatga tgcctttaaa gccacggatg
    10261 gtgtctttca gggagacgta tttacccgga gaaccggtga atacttctgc cacgaagaac
    10321 ggctgggaca ggaagcgctg gatcttacga gcacgcgcta ccaccagttt gtcttcttca
    10381 gacagttcat ccatacccaa gatggcgatg atgtctttca gttcctgata acgttgcagg
    10441 atggactgaa cgccacgcgc ggtgtcgtag tgttcctgac caaccaccag cgggtccagc
    10501 tgacggctgg tggagtccag cgggtcaacg gccgggtaga tacccagaga cgcgatctga
    10561 cggctcagta ccacggttgc gtcaaggtgc gcaaaggtgg ttgccggaga cgggtcagtc
    10621 aagtcatccg caggtacgta taccgcctgt acggaggtga tagaaccagt tttggtggag
    10681 gtgatacgtt cctgcagaac gcccatctct tccgccaggg tcggctgata acctaccgct
    10741 gaaggcatac ggcccagcag tgcggatact tccgtaccgg ccagggtgta acgatagatg
    10801 ttgtcaacga acagcagaac gtcacgacct tcgtcacgga atttctcagc catggtcaga
    10861 ccggtcagag caacgcgcag acggtttccc ggcggctcgt tcatctggcc atacaccagg
    10921 gatactttgt cgataacgtt ggagtcggtc atttcgtggt agaagtcgtt accctcacga
    10981 gtacgttcac ctacgcccgc aaacacagag taaccggagt gctcgatcgc gatgttacga
    11041 atgagctcca tcatgtttac ggttttacct acacccgcac caccgaacag accaacttta
    11101 ccgcccttag cgaacggaca catcaggtcg ataactttga taccggtttc cagcagttcc
    11161 tgagagtttg acagctcttc gtaggaaggt gctgcgcggt gaatcgccca acgctcttct
    11221 tcaccgatct cgcctttcat gtcgaccggt tcacccagta cgttcatgat acggcccaga
    11281 gtcgctttac ctaccgggac ttcgatcggg tgttcgaggt cttttacatc cagaccgcga
    11341 cgcagaccgt cggaggaacc cattgcgatg gtacgcacga taccgccgcc gagctgctgc
    11401 tgaacttcca gcaccagacg ctcattacca ttttgcacct caagagcatc gtacacgcgc
    11461 ggtacggcat cctgagggaa ttcgacgtca actacggcgc cgattacctg gacaatcttt
    11521 ccagtagcca tcttaaatcc tctacgaaat aacctgttta aaccgcggcg gcccccgaga
    11581 cgatctcggt gagttcctga gtaatgctgg cctgacgagc tttgttgtat accaactgca
    11641 gctctttaat caggctgccg ccattgtcgg tcgcggcttt catcgccacc atacgggcgg
    11701 cctgctcgct ggccaggttt tcaaccacgc cctgataaac ctgagattcg acataacgac
    11761 gcagcagggt atccagcaac gccttcggat cgggttcgta caggtaatcc caggatttat
    11821 gtttcagatc atcatcatct gatgccggta acggcagcag ctggctgatg gtcggaacct
    11881 gagacatggt gttaataaat ttgttgctga caatgtaaag cttgtccaga cggccttcgt
    11941 cgtaagcctg caacatcact tttaccggac cgatcagttc ggacagggaa gggttatccc
    12001 ccatgccggt gacctgggca acaacattgc cgcccacgga gttgaagaac gacacgcctt
    12061 tcgagccgat cattgcgagg tcgcattgaa cgcttttgtc ggtccaggtc ttcatttccg
    12121 ccagtagttt tttgaacagg ttaatgttca aaccaccgca caaaccacgg tcggtcgaca
    12181 ccaccaggta gcccacgcgt ttaacgtcgc ggtcttccag gtaagggtgc ttatattcca
    12241 gattaccgtg tgcaaggtga ccaatcactt tgcgcatggt ttctgcataa ggacggctgg
    12301 ccgccatgcg atcctgcgat ttacgcattt tggaagcggc gaccatctcc atcgctttag
    12361 tgatcttttg cgtgttctgg acgcttgcga tcttactacg tatctctttt gcgccggcca
    12421 tgagcttctc ctcaatgcct tgcggcctgc cctaaggcaa gccgccagac gttaccagga
    12481 ttgggttgct ttgaaggaat cgaggatgcc tttcagcttg ccttcgattt cgtcgttgta
    12541 gccaccggtc tggttgatct cttgcatcaa cggagcgtga tcacggtcga cgtaagccag
    12601 cagagcggct tcgaagctgc caattttcga cagttcaaca tccgccaggt aaccacgttc
    12661 tgctgcgaac agaaccagag actgctgcgc aacggacatc ggcgcatact gtttctgttt
    12721 cagcagttcg gtcactttct gaccgtggtc aagctgctta cgtgttgcat cgtcaaggtc
    12781 ggatgcaaac tgagagaacg ctgccagttc acgatactgt gccagagcgg tacggatacc
    12841 accggacagt tttttcatga tcttggtctg tgctgcacca ccaacacggg atacggaaat
    12901 acccgggtta accgcaggac gaataccggc gttgaacagg ttggtttcca ggaagatctg
    12961 accatcggta atggagatta cgttggtcgg aacgaacgca gaaacgtcac ccgcctgagt
    13021 ttcgataatc ggcagtgcgg tcagagaacc ggttttccct ttcacttcac ctttggtgaa
    13081 ggcttcaacg tattcggcgt taacacgtgc agcacgctcc agcagacgag agtggaggta
    13141 gaaaacgtcg cccgggaatg cttcacgtcc tggcggacga cggagcagca gggagatctg
    13201 acggtaagca acagcctgtt tagacaggtc atcgtaaatg atcagcgcat cttcaccgcg
    13261 gtcacggaag tattcgccca ttgcgcaacc ggcatacggt gccaggtatt gcagtgcagc
    13321 ggattcagac gcggttgcta ccacaacgat ggtgttagcc agtgcgccgt gctcttccag
    13381 tttacgtacc acgttagaaa tggtggacgc tttctggccg atagcgacat agatacattt
    13441 gataccggaa tcgcgctggt tgatgatggc atcgatagcc agtgcggttt tacctgtctg
    13501 acggtcaccg atgatcaatt cacgctgacc acgaccgatt gggatcatgg agtcaacggc
    13561 tttataaccg gtctgtaccg gctgatctac ggactgacgt tcgataacgc ccggagcgat
    13621 tgcttctaca gcagagaagc cgtcgtgatc cagcggacct ttaccgtcga ttggtgcacc
    13681 cagagtgtta accacacggc ccagcaggcc acggccaacc ggaacttcca ggatacggcc
    13741 agtacactta actttcatgc cttcggcaag gtcagcgtac ggacccataa caaccgcacc
    13801 tacagagtcg cgctcaaggt tcagtgcgat agcgtaacgg ttacccggca gggagatcat
    13861 ttcaccctgc atacaatcgg ccaggccgtg aatgcggata acaccgtcac ttacagaaac
    13921 aatagtacct tcgttgtgag cttcactcac aacattgaac tgagcaatgc gctgcttgat
    13981 cagttcgctg atttcggtgg aattcagttg catgctccag tccccttaag actgcaagac
    14041 gtctgcaagg cgctcaagac gaccgcgtac gctgccatca atgaccatat cacccgctcg
    14101 gatgataacg cctgccatta cagacttatc gattttgcaa ttcagcttaa ctttgcgtga
    14161 cagacgtttt tccatcgcag cagaaatttt cgcgagctgt tgttcactca gtgcggcagc
    14221 ggaaatgacg tctacctcag cggtagcctc actcacggca cgaaggtgaa taaactgctc
    14281 cagaacatcc gggagcgcgt taagacgacc attttcagcc attacccgaa tcaggttctg
    14341 accgttttcg tccagttgct caccacaaac tgcgataaac gactcggcga gcgtttctgg
    14401 cgcaagcgcg ccagagagaa gctctgccat ttgttcgttt ttggttacct cggcggcaaa
    14461 cgccagcatg tcctgccagc gttctacact ttggtgttcg acggcaaagt caaaagctgc
    14521 tttggcgtag gggcgagcta ccgtaataaa ttcagacatc agcccctccc tccttacagt
    14581 tcagcgacaa gtttatccac gatgtcgctg ttagcagctt catccacgga acgttcgatg
    14641 atcttctcgg cgccagcaac agccaggata gcaacttgct tacgcagctc ttcacgggca
    14701 cgtttacgct cggcttcaat ttctgcctgc gcctgggcca cgattttagt acgttcctgt
    14761 tctgcctcag ctttcgcttc gtccagaatc tgcgagcggc gtttgttcgc ctgctcgatg
    14821 attacctggg cttccgcttt cgcttttttc agctggtcgg tcgcgctggc ctttgcaagg
    14881 tcaaggtcct tatgtgctcg ttctgcggaa gcaaggccgt cagcaatttc tttttgacgt
    14941 ttttcgatgg ctgccattaa tggcggccat acgtacttca tgcagaacag aacgaacagg
    15001 acaaacgcga tggcctggcc gaggattgtt gcgttaagat tcacagcaca atgcctctat
    15061 ttagttaacg ttctgatatt gctctttaaa taaaagcaac gcttactacg cgacagcgaa
    15121 catcacgtac agacccagac ctacagcgat catcgggata gcatccacca gacccataac
    15181 gataaagaac tgagtacgca gcagaggaat cagatcaggt tgacgcgctg cgccttccag
    15241 gaatttaccc ccgaggatgc cgataccgat cgcagcaccg attgccgcca gacccatcat
    15301 cacagcggca gccatgtaca gcagatccat attcaggttt tccatgacag tctccagttt
    15361 gtttcagtta aaacgtagta gtgttggtaa attaatgttc ttcagacgcc atcgacagat
    15421 agacgatcgt cagaaccatg aagatgaagg cttgcagcgt aatgatcagg atgtggaaaa
    15481 tggcccacgg cacattcagg atccactgtg accaccacgg caacagacca gcaatcagaa
    15541 tgaaaatcag ctcaccggca tacatgttac cgaacagtcg caaaccgagt gaaactggtt
    15601 tggacagcag gcttacccct tcaaggatta agttgacagg aatgaacgcc cagtgattga
    15661 acggctgcag cgtcaactct ttcgtgaagc cgccgatgcc tttcattttg atgctgtaga
    15721 acagaatcag gataaatacg cccagtgcca tagacagcgt tacgttcacg tccgcagacg
    15781 gaaccacacg cagtgcaggc agacccagta catgttcagc aatgtacggc agcaggtcga
    15841 taggcagtaa atccatcagg ttcatcagga atacccagac gaagatcgtc agggccagcg
    15901 gagcaatcag cttgcttttg ccatggtaca tgtctttcac gctaccatta acaaagccga
    15961 tcaccagctc aatcgcggtc tgaaacttac ctggcacacc gctggtcgcc tttttggcta
    16021 cgctacggaa taaaaccagg aacaacagac ccagcaccac cgagaagaac atggagtcaa
    16081 tattgattgt ccagaaggtg gctggggggt tttgtggatc caccagcgag aatgtacgca
    16141 ggtccagctg aaggttattc aggtggtgtc ctatgtaatc ctgcggcgtc atattttctg
    16201 aagccatgat gccttttacc ctttgttgtt aattacagcc ggtgccagta tctgaaccac
    16261 cagcaccaaa acccacgtaa cgatcagcgg caagaatacc gcctttaaaa ccgccaacgc
    16321 caccaccagt aacaccaaca tcgccagaac tttgaaagct tcgccaaatg cgaatgtcca
    16381 ggccacccgg cctttcgctg gtgtatacgc ctggtgacgc caggcaaata tcataaacaa
    16441 aacgttaggc agaaagactg ccaggccccc gcttattgca gagacgcccc agaaggggtc
    16501 tttgaggctg aacagcaatc cacttgctat caccaccagt aactgaacga gcagaagctt
    16561 ccgagcaacg tttcgactca cgagcgacac agacatcacg tttttcactc ctgctccctt
    16621 cgaggtatgc cgcgtgtcgt ataaaacttt ctttaaggct tagagtcaag catcaaaaag
    16681 cggtcaaatt atacggtgcg cccccgtgat ttcaaacaat aagtagccaa aaggtgaata
    16741 aatgtttaaa tatttttcca gtgcatacaa ttgcgacttt tccgctaacc ctgttcgatc
    16801 atgaaaaact gtaaataacg cgtaaacact agtgataaag cgtgcttcag atcacatatt
    16861 gcgcatgttc gcgcacagta tatttattta cttggcaaat gatgcctttg caagtttatg
    16921 atatttcagt ctaaaaacag atactgtttt aataaatgac atttacacaa caaaaaccac
    16981 ccattgacat ttttaataat gttttaacag ccaatgatgg ttcttagcgc cgatttttag
    17041 cagactgata ttttcactaa tgacttattt tctgcttacc aaaaaaagcc acgttatctt
    17101 gttgatacaa aagagtgaac gtggcgttaa atgtaaccag ttatatcagt agaaaacttg
    17161 gttgttgtta acagtctaac cggtcaattt tttatgattt ttttgataaa aattaaattt
    17221 tatttgcttt aatcaccacc agatgacgtt cgccatccag agctggaacc tgaagtttaa
    17281 ccactgattc gacctgatat tcttcgggca acaaagcgat ttcatcttcc ggcatttgcc
    17341 ctttcagcgc gtagaaacgg ccttgctcac caggaagatg gtggcaccag ctcaccatat
    17401 cgttcagaga ggcaaaagcg cggctaatta cgccatcaaa tggcggctct gaaggaaact
    17461 cttctaccct gctctgtact ggctcaatat tctccagttt aagctcatgt tgcacctgac
    17521 gaaggaaacg cacgcgttta ccaaggctat ccaacagagt gaaatgggct tcaggacgca
    17581 cgatagagag tggaatgcct ggcagtcctg gtccggtgcc gacatcgata aaccgttcac
    17641 cttgcagata cggtgccacc acaatgctat cgagaatatg gcgtaccagc atctcattag
    17701 gatcgcggac cgaagtcagg ttgtacgctt tgttccattt atgcagcata ttcacgtagg
    17761 caataagctg gtttttctgg tgatcggtaa gcgaaatacc tgcgtctttc agcagtaagg
    17821 agagtttgtt gagcacggtg attacctgtt cttgatgcgt tgcctggtaa gcgggtgctt
    17881 accaggcatt tttaatgcgt tatgcgctac gacgcagcat accctgtttt ttcagccaca
    17941 ccagcagaat ggagatagcc gcaggcgtga cgccagaaat acgcgaagcc tggccgatag
    18001 aggccggttt gtgatcgtta agtttggcga tcacttcgtt agaaagaccg gatacctggc
    18061 ggtaatccag tgtcgcgggt agcagggtgt tctcgttacg cagctgcttt tcgatctcat
    18121 cttgctggcg cgcgatataa ccttcgtatt taacctgaat ctcaacctgt tccgccgcct
    18181 gttcgtctgt caacgcaggg gcaaacggcg tcagcgtggt taatttttca taagtcattt
    18241 ccggacgacg cagcagatct tcaccactgg cttcacggga aagcggcgca gtcaggtgag
    18301 cattcacttc ggctgcagct tccgccgacg gggttaccca ggtcgatttc agacgctgac
    18361 gctcacgctc gatattctca agtttctcgt taaagcgcgc ccaacgttcg tcatccacca
    18421 ggcccagttc acgaccgatt tcagtcaaac gcagatccgc attatcttcg cgcagcatca
    18481 gacgatattc tgcgcgcgaa gtaaacatac gatacggttc tttggttcct aaagtgcaca
    18541 ggtcatcaac cagtacgccg agatacgcct gagaacgcgc cggagcccaa ccttctttgt
    18601 cagcagacag acgggcagcg ttaagaccga ccagcaaacc ttgcgcagcg gcttcttcgt
    18661 aaccggtagt gccgttaatc tgaccagcaa agaacagccc ctggataaac ttgctctcca
    18721 gcgtcggttt caggtcgcgt ggatcgaaga agtcatactc aatggcataa cccggacgca
    18781 cgatcttcgc gttttccatc ccctgcatgg agcggacgat ttgcatctgc acatcgaacg
    18841 gcaggctggt ggagataccg ttcggataaa tttcgttaga ggtcagccct tccggttcaa
    18901 ggaagatctg atgctgattt ctgtcggcga agcgcatgac tttgtcttcg atcgatgggc
    18961 agtagcgtgg gccgacacct tcgatcaccc ctgcgtacat tgggctacga tcgaggttac
    19021 tgcggatcac atcatgggtt ttctcgttgg tatgagtgat ataacacggc acctgctggg
    19081 gatgctggga cgcattgccc ataaacgaga ataccggcat tgggttatcg ccatgctgtt
    19141 gcgccagcac actaaagtcg atagttcgag catcaatacg cggtggtgtc ccggttttca
    19201 gacgaccaac gcgcagcggc agttcacgca aacggcgaga aagcggaatg gacggcggat
    19261 caccagcacg accaccgctg taattatcca gaccgatatg aattttaccg tcgaggaacg
    19321 tcccaacggt gagcacgacg gctttggcac ggaacttcag tcccatttgg gtaacagcac
    19381 cgaccacgcg atcgttttcg acaataagat cttcaaccgc ctgctggaag atcatcaggt
    19441 tcggttggtt ctccagcgcc gtacgtaccg cctgacggta gagcacacga tccgcctgag
    19501 ctcgggtagc gcgaaccgcc ggtcctttgc ttgcgtttag tatcctaaac tggatacccg
    19561 cttgatcgat cgctttcgcc atcagaccgc cgagtgcatc cacttctttt accagatgtc
    19621 ccttcccaat accgccgatc gccgggttgc agctcatctg ccccagagtg tcgatattgt
    19681 gtgtcaaaag cagagtctgt tgacccatac gcgccgcggc catcgcggcc tcggtgcctg
    19741 catgaccccc gccaatgatg atgacgtcaa aaggatccgg ataaaacatg gtgattgcct
    19801 cgcataacgc ggtatgaaaa tggattgaag cccgggccgt ggattctact caactttgtc
    19861 ggcttgagaa agacctggga tcctgggtat taaaaagaag atctatttat ttagagatct
    19921 gttctattgt gatctcttat taggatcgca ctaccctgtg gataacaagg atccggcttt
    19981 taagatcaac aacctggaaa ggatcattaa ctgtgaatga tcggtgatcc tggaccgtat
    20041 aagctgggat cagaatgagg ggttatacac aactcaaaaa ctgaacaacc gttgttcctt
    20101 ggataactac cggttgatcc aagcttcctg acagagttat ccacagtaga tcgcacgatc
    20161 tgtatactta tttgagtaaa ttaatccacg atcccagcca ttcttctgcc ggatcttccg
    20221 gaatgtcgtg atcaagaatg ttgatcttca gtgtttcgcc tgtctgtttt gcaccggaat
    20281 ttttgagttc tgcctcgagt ttatcgatag ccccacaaaa ggtgtcatat tcacgactac
    20341 caataccgat tgcgccaaag cggaccgcag aaagatcggg cttctgttcc tgcaatgctt
    20401 catagaaagg agaaaggttg tccggaatat ctccggcacc gtgggtggag ctgataatca
    20461 gccagatccc cgaggcagat aaatcttcca acagcggacc gtgcagcgtt tcggtggtaa
    20521 aacccgcctc ttccagcttt tcagccaggt gttctgctac atattcggca ccgccgaggg
    20581 tgctgccgct gataagagtg atatctgcca taaaccgcca cctttattaa gagtggcgta
    20641 ttgtacgctg tgaacgcgtt gggatctacc tgtggaaaag tatgggatta aaaaagccga
    20701 ttatggcttg atggtacgca tgatcgggtt ttgcaggacg atcaatgtct cggtggactg
    20761 aatttcatca attgtttgga tcttgttgat aagtacatgc tggagagcgt cgatcgaacg
    20821 gcacattact tttataaaga tgctgtagtg gcctgttgtg taataggctt cagtgacttc
    20881 agcaaggctt tccagctttg ccagcgcgga agggtagtct ttggcgctct ttaatataat
    20941 gccgataaag cagcctacgt cataaccgag ctgcttcggg ctgacatcaa tacgcgcccc
    21001 ggtaatgatc cccgcctgct tcattttctc tactcgaacg tgaatcgtcc ccggactgac
    21061 gccaaattgt ttcgccagtt cggcgtaagc ggtgcgcgca ttgcccatta atgcttccag
    21121 gatgccacgg tccagattgt cgatcagata attttccata ggattttctt atgcagattg
    21181 atgattcatt ctattttagc cttctttttt aatgaatcaa aagtgtgtta ggctttttat
    21241 tgaatgatta ttgcatgttt gtcggttttt gttgcttaat cataagcaac aggacgcagg
    21301 agtataaaaa atgaaaaccg cttacattgc caaacaacgc caaattagct tcgtgaaatc
    21361 tcacttttct cgccaactgg aagaacgtct ggggctgatc gaagtccagg cgccgattct
    21421 tagccgtgtg ggggatggca cgcaggataa cttgtcgggc tgtgaaaaag cggtgcaggt
    21481 aaaagtgaaa gctctgcctg atgcccagtt cgaagtggtt cattcactgg cgaagtggaa
    21541 acgtcagacc ttagggcaac acgacttcag cgcgggcgaa gggctgtaca cgcacatgaa
    21601 agcccttcgc cccgatgaag accgtctttc tccgttgcac tcggtctatg ttgaccagtg
    21661 ggactgggaa cgcgtaatgg gcgacggtga gcgtcaattc tcgactctga aaagcacggt
    21721 agaggcgatc tgggcgggaa ttaaagcaac cgaagctgcg gttagcgaag agtttggcct
    21781 ggcaccgttc ctgccggatc agatccactt cgtacacagc caggagttac tgtctcgtta
    21841 tccggatctc gatgccaaag ggcgtgagcg ggcgatagcg aaagatcttg gcgcggtatt
    21901 ccttgtcggg attggcggca agctgagcga tggtcatcgc cacgacgtgc gcgcaccgga
    21961 ttatgatgac tggagcaccc cgtcagagct gggtcatgcg ggtctgaacg gcgatattct
    22021 ggtgtggaac cctgtactgg aagatgcgtt tgagctttct tccatgggga tccgcgtgga
    22081 tgccgacacg ttgaagcatc agttggcgct gaccggtgat gaagatcgtc tgcagctgga
    22141 atggcatcag gcgctgctgc gcggtgaaat gccgcagacc atcggcggcg gtatcggcca
    22201 gtctcgtttg accatgctgc tgctgcaact gccgcatatc ggccaggttc agtgtggtgt
    22261 atggccagct gctgttcgcg agaacgtccc ttctctgctg taataattta tcgccgccag
    22321 cgtctgagca ggcggcttcg catcccggta tcaaagcgcc agatatgatc gaaaatgcgc
    22381 atgatgccgg gtttgccgtg tgccgacatc gccacggcat gaaagcgatg ctgatgtacc
    22441 cgctgcagct ctttcacttt actcgtcacg tcgtcaggca accgctgagc gataaaatca
    22501 gaaatcacca ccgcatcggc atcaaaccat tccctgcttt gcaagcgttc cataatggcg
    22561 cgaaaacaac tggcaagatc ggtgccgcca cgaaactgct ggcttaaaaa acggattgct
    22621 tgttcgatgc cttgggggcc tgaaagctca taacggacga tctcggtgga aaatagcata
    22681 atatagcagc gccggttttc agcgagagca atgcgcatca aggccaggca gaacgctttc
    22741 gcacactgtt cattaaagcc gcccattgag ccggaagtat ccacacagac aataaacggc
    22801 ccgcgcggct gttcatcgta atctttatgt accaccggac gttcgatcac tttttcacgc
    22861 cacgactcac cgtgcaggcg ataggtgagc aactgttttt ccaccagccg acggtaaaac
    22921 tcatactcca gttccgttat ccctagtgtc gccagttctg gcggcagaag acgtaaaata
    22981 tcatcgcttt gttgcagacc atcaacctgc tcaggaaccg tcgccggttc gcgcaccatg
    23041 gtgcggaagg tttccatctg cgcatcgttg cgcggtattg atttggcttc ccgagaacgc
    23101 cccagctgct ctgccaggcg tttcagttcc ggctgttcgt taagaaattc accgtatttc
    23161 acaatcaact gatagtcgcc acgtttaagc tgaccggcgc tcatatccca cagacgacca
    23221 gctgcagtat tgttatctgc gagaatcggt tcaagttgtc cgctcagcgt catgcgttct
    23281 tgaacttcac tcaacagttg ttcgcgttct tcttctaata gctgttgatt taacgtcgtt
    23341 gcttgcacga tcagacttaa acgccaacgc tggagaaaaa gcgtgtgtaa cgctgaagtt
    23401 attgtgctgt tagcatcaac caactgccgg gcttgttctg cccatgggga attcagacga
    23461 tgcagtaagt ccaggatctg tggtagctgc acaataaact gcggcgtgga gaggagctgg
    23521 ctttgctgat agcacatcac ctcttcggtg agttccggcg ggactcgggc atctttcagc
    23581 cgactgcgca gcgcctcacg ccagcgggga acatcatcag tgattgccgc cttcaggcgt
    23641 gggaattttt caaagaagac cgccagctgc ggtgaggcca gcagtgcgat gatcatctct
    23701 tcgatcaatc cctcttcgct gacggccagc atcacattaa gcgtatccag cgttagcatt
    23761 gttgtgcctg gtgaatctgt gcaccgacat cctgtaggct ggcttcaatg cgacctaacc
    23821 agtcgccagg gataaacaga catttttgct gttcgctgaa taaagcgtgt tgcttgcgcc
    23881 agtcgctttc aagctcttcc agttgttgtt ttatttcacc aggcagacct tcagccgatg
    23941 aaccgggaag tgccagcgta ctgccttgta aacttacatc gcgaacgaca agatgttggg
    24001 cgctatcaac ttccagattc agtttctggg caaagccgat accgttcagt ttaccgcgaa
    24061 tttcaccacc tttgctcagc cactgttcca gcgcgctacg ctcaaaggag atatgaacca
    24121 cttccatatc atgcagtttt aacggttttt gcagcagtag agtcagagtg gaagcagtaa
    24181 cgttaacagg aagttgatac tgctgacgac ggctgaaaat gccgcccaga cgaattaccg
    24241 ttaaggctgt tttatcgctt tgttgctgct ggagttgcag gtgacgttgc acaatcgcgc
    24301 caaggcgagt caacatccct tgctgttgcc aggcatgacc ggtcatcaat acatcaattt
    24361 gttgttgtat caaattcagg ctttgcgcgt cataccacag gcaatctttc agcaaaatga
    24421 gatctaccgg ggcaacagca ctgcgaccgc taaaaaaggc gctggcctgc aataatcgga
    24481 tcgctttttt ccagcgacga tccgagacat aaggcgcatc cggtaattta tccagttgca
    24541 ggcgcagcat aaaaatcagc tcaaatacat gatcgggcag cgtaatttca ccaatctctt
    24601 tctgccagcg ttcatattct tcatctgtga cctgcaaggc atcaggaacc ggattgtcgt
    24661 tttcatcctg ttgactggtc agcatggagc ggaaattcgc tttatcctgc actttatcta
    24721 accacagacg aatcagcatg cggtcatata acgcttccag actgctgtct gcttccggca
    24781 gctcgttgga ggccgccacc agcagacgca tcgggatttt ttctacgtgt gcaccgttgc
    24841 ggaactggcg ctcgttaatg gcggtgagca aggtattaag aattgccggg cccgctttcc
    24901 agatctcatc cagaaagaca atttctgctt ccggcaggta accgctggtt aaacgttcat
    24961 agcgcccttc atcttttagc gcctgaatag aaaggggacc aaaaacttct tccggcgtgg
    25021 agaagcgggt catcagatat tcaaacgcgc gggcattctg aaaggcgaat tttaagcgcc
    25081 gggcgatcaa acttttggca atacctggcg ggccaaggag gaacacactt tcaccactta
    25141 atgccgctaa taaacacaag cggatggcgt ggctacgttc ataaagcccc ttttccaacg
    25201 aactgctcag gcgggaaatt ctttccgcta ataaatgagg gtgagccata atgaagtggc
    25261 gtcctttcgt caaaagttct gcgtaaattg cgagtataga cgtttcttgc tggtggctaa
    25321 aatagtctca aag
//
LOCUS       DAJVQG010000049        25104 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJVQG010000049 DAJVQG010000000
VERSION     DAJVQG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.049"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            194..382
                     /locus_tag="OQM83_001799"
     CDS             194..382
                     /locus_tag="OQM83_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCS1520081.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            394..1146
                     /gene="bcsQ"
                     /locus_tag="OQM83_001800"
     CDS             394..1146
                     /gene="bcsQ"
                     /locus_tag="OQM83_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCS1520082.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1143..1307
                     /locus_tag="OQM83_001801"
     CDS             1143..1307
                     /locus_tag="OQM83_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCS1520083.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1500..3761
                     /gene="bcsA"
                     /locus_tag="OQM83_001802"
     CDS             1500..3761
                     /gene="bcsA"
                     /locus_tag="OQM83_001802"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HCS1520084.1"
                     /translation="MNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSLILA
                     LICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRYTST
                     LNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVDIFV
                     PTYNENLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIARTTHE
                     HAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHFFSP
                     DPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSYAVIRRKPLDEIGGIAVET
                     VTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLDNPL
                     TGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFVLPH
                     MIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGGLVE
                     EEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVLGGA
                     VAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQILEGQ
                     KVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWALWQD
                     SYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRHPERS
                     ETAQPSDQALAQQ"
     gene            3772..6111
                     /gene="bcsB"
                     /locus_tag="OQM83_001803"
     CDS             3772..6111
                     /gene="bcsB"
                     /locus_tag="OQM83_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCS1520085.1"
                     /translation="MKRKLFWICAVAMRMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKSDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6118..7224
                     /gene="bcsZ"
                     /locus_tag="OQM83_001804"
     CDS             6118..7224
                     /gene="bcsZ"
                     /locus_tag="OQM83_001804"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCS1520086.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7206..10698
                     /gene="bcsC"
                     /locus_tag="OQM83_001805"
                     /pseudo
     CDS             7206..10698
                     /gene="bcsC"
                     /locus_tag="OQM83_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10780..12768
                     /gene="hmsP"
                     /locus_tag="OQM83_001806"
     CDS             10780..12768
                     /gene="hmsP"
                     /locus_tag="OQM83_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCS1520087.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12951..14237
                     /gene="dctA"
                     /locus_tag="OQM83_001807"
     CDS             12951..14237
                     /gene="dctA"
                     /locus_tag="OQM83_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCS1520088.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14457..15953
                     /locus_tag="OQM83_001808"
     CDS             14457..15953
                     /locus_tag="OQM83_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCS1520089.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16049..16978)
                     /gene="kdgK"
                     /locus_tag="OQM83_001809"
     CDS             complement(16049..16978)
                     /gene="kdgK"
                     /locus_tag="OQM83_001809"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCS1520090.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17210..17977
                     /gene="pdeH"
                     /locus_tag="OQM83_001810"
     CDS             17210..17977
                     /gene="pdeH"
                     /locus_tag="OQM83_001810"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCS1520091.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18047..20107
                     /locus_tag="OQM83_001811"
     CDS             18047..20107
                     /locus_tag="OQM83_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCS1520092.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWFKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIATNIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20289..21611)
                     /locus_tag="OQM83_001812"
     CDS             complement(20289..21611)
                     /locus_tag="OQM83_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCS1520093.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22022..23035)
                     /gene="yhjD"
                     /locus_tag="OQM83_001813"
     CDS             complement(22022..23035)
                     /gene="yhjD"
                     /locus_tag="OQM83_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCS1520094.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23084..23983)
                     /locus_tag="OQM83_001814"
     CDS             complement(23084..23983)
                     /locus_tag="OQM83_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1520095.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24503..>25104
                     /locus_tag="OQM83_001815"
     CDS             24503..>25104
                     /locus_tag="OQM83_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS1520096.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5894 a         6365 c         6991 g         5854 t
ORIGIN      
        1 ttagcttgtg aatcaattta gaaggtcaca agacgtgtga ttttagtctt ttcgttttta
       61 actttttctg ttgcgtaacg gaaagtcaaa aagtgagcaa attcccgtct cgccgtaaac
      121 aataaaccgg cgaaatgctc acggttagaa ttgtttcatg aacggtacgg ttatttcata
      181 gggatcaagc aaaatgaata acaatgaacc agatactctg cctgatcccg cgataggcta
      241 tatcttccag aatgatattg tggcgttaaa gcaggcattt tcactgcctg atattgatta
      301 tgccgatatt tcccaacgcg aacagttggc cgcggcatta aaacgctggc cgttgctggc
      361 agagtttgcg caacaaaagt aggggattgg tgaatggccg tactgggatt gcagggggtg
      421 cggggaggcg tggggacaac aaccatcacc gccgcattag cctggtcatt acaaatgttg
      481 ggagaaaatg tcctggtggt cgatgcctgc ccggacaact tgttgcgcct gtcatttaat
      541 gttgatttta cccaccgtca gggctgggcc agagcgatgc tggatggcca ggactggcgt
      601 gacgctgggt tgcgctacac ctcgcagctc gatttgctgc cttttggtca gttatccatt
      661 gaagaacaag aaaatccaca gcactggcaa acccggctga gcgatatttg ctccggctta
      721 cagcaactaa aagccagcgg gcgttaccag tggattttaa tcgacttacc gcgtgatgcc
      781 tcgcagataa cccaccagct gctgagtttg tgcgatcact cgctggcaat cgtcaatgtg
      841 gatgccaact gccatatccg actgcatcag caagcgctgc cggatggcgc acatattttg
      901 attaatgact tccgtattgg cagtcaggtt caggacgata tttaccagct ttggttgcaa
      961 agccagcgcc gattactgcc gatgctcatt catcgtgatg aagcgatggc tgaatgcctg
     1021 gcggctaagc aaccagtagg tgaatatcgc agtgatgcgc tggcggctga agagatactg
     1081 acgctggcga actggtgcct gttgaactac tccgggctga aaacgccagt cgggagtgca
     1141 tcatgagtat cctgacccgg tggttgctta tcccgccggt caacgcgcgg cttatcgggc
     1201 gttatcgcga ttatcgtcgt cacggtgcgt cggctttcag cgcgacgctc ggctgtttct
     1261 ggatgatcct ggcctggatt tttattccgc tggagcaccc gcgctagcag cgtattcgcg
     1321 cagaacataa aaacctgtat ccgcatatca acgcctcgcg tccgcgtccg ctggacccgg
     1381 tccgttatct cattcaaaca tgctggttac tgatcggtac atcgcgcaaa gaaacgccga
     1441 aaccgcgcag gcgggcattt tcaggtctgc agaatattcg tggacgttac catcaataga
     1501 tgaacgagct gcctgagcgc gttagccata aaacacagca tcttgatgag aaaaaagagc
     1561 tcggtcattt gagtgccggg gcgcggcggt tgatcctcgg tatcatcgtc accttctcgc
     1621 tgattctggc gttaatctgc gttactcaac cgtttaaccc gctggcgcag tttatcttcc
     1681 tgatgctgct gtggggtgta gcgctgatcg tacggcggat gccggggcgc ttctcggcgc
     1741 taatgttgat tgtgctgtcg ctgaccgttt cttgccgtta tatctggtgg cgttacacct
     1801 ctacgctgaa ctgggacgat ccggtcagcc tggtgtgcgg gcttattctg ctcttcgctg
     1861 aaacgtacgc gtggattgtg ctggtgctcg gctacttcca ggtagtatgg ccgctgaatc
     1921 gtcagccggt gccattgccg aaggatatgt cgctgtggcc gtcagtggat atctttgtcc
     1981 cgacttacaa cgaaaatctc aatgtggtga aaaataccat ttacgcctcg ctgggtatcg
     2041 actggccgaa agacaaactg aacatctgga tcctcgatga cggcggcagg gaagagtttc
     2101 gccagtttgc gcaaaacgtg ggggtgaaat atatcgcccg caccactcat gaacatgcga
     2161 aagcgggcaa catcaacaat gcgctgaaat atgccaaagg cgagttcgtg tcgattttcg
     2221 actgcgacca cgtaccaacg cgatcgttcc tgcaaatgac catgggctgg ttcctgaaag
     2281 agaaacagct ggcgatgatg cagactccac accacttctt ctcgccggac ccgtttgaac
     2341 gcaacctggg acgttttcgt aaaacgccga acgaaggcac gctgttctat ggtctggtgc
     2401 aggatggcaa cgatatgtgg gacgccactt tcttctgcgg ttcctatgcg gtgattcgtc
     2461 gtaagccgct ggatgaaatt ggcggcattg ctgtcgaaac cgtgactgaa gatgcgcata
     2521 cttctctgcg tttgcaccgt cgtggctata cctccgcgta tatgcgtatt ccgcaggcgg
     2581 cggggctggc gaccgaaagt ctgtcggcgc atatcggtca gcgtattcgc tgggcgcgcg
     2641 ggatggtaca aatcttccgt ctcgataacc cgctcaccgg taaaggactg aagtttgctc
     2701 agcggctgtg ctacgtcaac gccatgttcc acttcttgtc gggcattcca cggctgatct
     2761 tcctgactgc gccgctggcg ttcctgctgc ttcatgccta catcatctat gcgccagcgt
     2821 tgatgatcgc cctattcgtg ctgccgcata tgatccatgc cagcctgacc aactcgaaga
     2881 tccagggcaa atatcgccac tctttttgga gtgaaatcta cgaaacggtg ctggcgtggt
     2941 atatcgcacc accgacgctg gtggcgctga ttaacccgca caaaggcaaa tttaacgtca
     3001 ccgccaaagg tggactggtg gaagaagagt acgtcgactg ggtgatctcg cggccctaca
     3061 tcttccttgt tctactcaac ctggtgggcg ttgcggtagg catctggcgc tacttctatg
     3121 gcccgccaac cgagatgctc accgtggtcg tcagtatggt gtgggtgttc tacaacctga
     3181 ttgttcttgg cggcgcagtt gcggtatcgg tagaaagcaa acaggtacgc cgatcgcacc
     3241 gcgtggagat gacgatgccc gcggcaattg cccgcgaaga tggtcacctc ttctcgtgta
     3301 ccgttcagga tttctccgac ggtggtttgg ggatcaagat caacggtcag gcgcagattc
     3361 tggaagggca gaaagtgaat ctgttgctta aacgcggtca gcaggaatac gtcttcccga
     3421 cccaggtggc gcgcgtgatg ggtaatgaag ttgggctgaa attaatgccg ctcaccaccc
     3481 agcaacatat cgattttgtg cagtgtacgt ttgcccgtgc ggatacatgg gcgctctggc
     3541 aggacagcta cccggaagat aagccgctgg aaagtctgct ggatattctg aagctcggct
     3601 tccgtggcta ccgccatctg gcggagtttg cgccttcttc ggtgaagggc atattccgtg
     3661 tgctgacttc tctggtttcc tgggttgtat cgtttattcc gcgccacccg gagcggagcg
     3721 aaacggcaca accatcggat caggctttgg ctcaacaatg atgataacgc gatgaaaaga
     3781 aaactattct ggatttgtgc agtggctatg aggatgagtg cgttcccctc tttcatgacg
     3841 caggcgacgc cagcaacgca accactgatc aatgctgagc cagctgtagc cgcccagacg
     3901 gaacaaaatc cgcaggtggg gcaagtgatg ccgggcgtgc agggcgctga tgcgccagtc
     3961 gtggcgcaga acggtccttc gcgtgatgtg aagctgacct ttgcgcaaat tgcgccgccg
     4021 ccgggcagca tggtgctacg tggcattaac ccgaacggca gcattgagtt tggtatgcgc
     4081 agcgatgaag tggtgacgaa ggcgatgctc aacctcgaat acacgccatc gccatcgtta
     4141 ctgcctgtcc agtcgcagtt aaaggtttat ctcaatgatg agctgatggg cgtgctgcca
     4201 gtgaccaaag aacagttggg taaaaaaacg ctggcgcaaa tgcccattaa cccactgttt
     4261 attaccgact tcaaccgtgt gcggctggag tttgtcggcc attatcagga cgtgtgcgaa
     4321 aacccggcca gcaccacgct ttggctggat gttgggcgga gcagtggact ggatctgacc
     4381 tatcagaccc tgaatgtgaa gagtgacctg tcacacttcc cggtgccatt ctttgacccg
     4441 cgcgataacc gtactaacac cttgccgatg gtctttgcgg gtgcgccgga tgttgggctg
     4501 caacaagcat ctgccattgt cgcctcgtgg tttggttcgc gttctggctg gcgtgggcag
     4561 aacttcccgg tgctctataa ccaattgccg gatcgcaatg ccattgtctt tgcaaccaac
     4621 gacaaacggc cggacttcct gcgcgatcat ccggcggtaa aagccccggt gattgagatg
     4681 attaaccatc cgcagaatcc ttacgtcaaa ctgctggtgg tgtttggtcg tgacgacaaa
     4741 gacctgttgc aggcagcgaa aggtatcgct cagggtaaca ttctgttccg tggtgaaagc
     4801 gtggtagtga atgaagtgaa accgctgcta ccgcgtaagc cgtacgatgc gccgaactgg
     4861 gtacgtaccg atcgtccggt cacatttggc gaactgaaaa cctatgaaga acagttacaa
     4921 tccagcggtc ttgagccagc agcgattaac gtttcgctaa acctgccgcc ggatctctac
     4981 ctgatgcgca gtaccggcat tgatatggat attaattacc gctacaccat gccgccggtg
     5041 aaagacagtt cgcggatgga tatcagcctg aataaccagt tcctgcaatc cttcaacctg
     5101 agcagtaaac aggaggcgaa ccgcctgctg ctgcggattc cggtattaca aggtttgctg
     5161 gatggcaaaa cggatgtctc tattccggcg ctgaaactgg gcgcgaccaa ccagctgcgc
     5221 ttcgactttg agtatatgaa cccgatgccg ggcggttcgg tggataactg tattaccttc
     5281 cagccggtgc agaatcatgt ggtgattggt gacgattcca ccatcgactt ctcgaagtat
     5341 taccacttca tcccgatgcc ggatctacgc gcctttgcta acgcgggctt cccgttcagc
     5401 cggatggcgg atctgtcgca aaccatcacc gtgatgccga aaacgcctaa cgaagcacag
     5461 atggaaacgt tgctgaatac tgttggtttt atcggcgcac agacgggctt cccggcgatt
     5521 aatctgacgg tgaccgatga tggcagcacc attcagggca aagatgccga catcatgatc
     5581 atcggtggta tcccggacaa attaaaagac gataagcaga tcgacctgtt ggtgcaggcg
     5641 accgaaagct gggtgaaaac accgatgcgc cagaccccgt tccccggcat tgtgccggac
     5701 gagagcgatc gtgcggcaga aacccagtca acgctgacct cttccggtgc gatggcggcg
     5761 gtgattggct tccagtcgcc gtataacgac cagcgcagcg tgattgcgct gctggcagat
     5821 agcccacgcg gttatgaaat gcttaacgat gcggtgaacg atagcggcaa acgcgccacc
     5881 atgttcggtt cggtcgcggt gatccgcgag tccggtatca acagcctacg tgttggcgac
     5941 gtttattacg taggtcatct gccgtggttc gagcgcttgt ggtatgcgct ggcaaaccat
     6001 ccgattctgc tggcggtgct ggcggcaatc agtgtgatat tgctggcatg ggtactgtgg
     6061 cgtctgctgc gaattattag tcgtcgtcgt cttaacccgg ataacgagta attgaagatg
     6121 aatgtgttgc gtagtggaat cgtgacgatg ctgctgttgg ctgcctttag tgttcaggca
     6181 gcctgtacct ggcctgcctg ggagcagttt aaaaaggatt acatcagtca ggaagggcgc
     6241 gtcatcgccc ccagcgacgc gcgcaaaatc accacctccg aagggcaaag ttacggtatg
     6301 ttctttgccc tggcggctaa cgaccgtgta gctttcgata atattctcga ctggacgcag
     6361 aacaatctcg ctcagggctc tttaaaagaa cgtttgcccg cctggctgtg gggcaagaaa
     6421 gagaacagta agtgggaagt gctggacagc aattcggcct ccgatggtga tgtctggatg
     6481 gcctggtcgt tgctggaggc ggggcgtttg tggaaagagc agcgttatac cgacatcggc
     6541 agcgcgttgc taaaacgtat cgcgcgggag gaagtggtga cggtgcctgg gctgggttcc
     6601 atgttgttac cgggcaaagt gggttttgct gaggataaca gctggcgttt taaccccagc
     6661 tacctgccgc cgacgctggc gcagtatttc acccgctttg gcgcgccgtg gaccacgctg
     6721 cgcgaaacca atcaacgttt attgctggaa accgccccga aaggtttttc gccagactgg
     6781 gtgcgctatg agaaagacaa aggctggcag ctaaaagccg aaaaaacatt gatcagcagc
     6841 tacgacgcta tccgcgttta catgtgggta ggcatgatgc ctgacagcga tccgcaaaaa
     6901 gcgcggatgc tcaaccggtt taaaccgatg gcgacattca ctgagaaaaa cggttatccg
     6961 ccggaaaaag tggatgtggc tacggggaaa gcgcagggta aaggaccgat cggtttttct
     7021 gccgccatgc tgcccttttt acaaaaccgc gatgcgcagg ccgttcagcg ccagcgcgtg
     7081 gccgataact ttcccggcag cgatgcctat tacaactatg tgctgaccct gtttggacaa
     7141 ggctgggatc aacaccgttt ccgcttctcg acaaaaggtg agttattacc tgactggggc
     7201 caggaatgcg caaattcaca ctaaacatat tcacgctttc cctcggtctg gccgtcatgc
     7261 cgatggtcga ggcagcacca accgctcagc aacagttgct ggagcaagtt cggttaggcg
     7321 aagcgaccca tcgtgaagat ctggtgcaac agtcgttata tcggctggaa cttattgatc
     7381 cgaataaccc ggacgtcgtt gccgcccgtt tccgttcttt gttacgtcag ggcgatattg
     7441 atggcgcgca aaaacagctc gatcggctgt cgcagttagc gccgagttca aatgcgtata
     7501 aatcgtcgcg gactacgatg ctactttcca cgccggatgg tcgtcaggca ctgcaacagg
     7561 cacgattgca ggcgacgacc ggtcatgcag aagaagctgt ggcgagttac aacaaactat
     7621 tcaacggtgc gccgccggaa ggtgacattg ctgtcgagta ctggagtacg gtggcgaaaa
     7681 ttccggctcg ccgtggcgaa gcgattaatc agttaaaacg catcaatgcg gatgcaccgg
     7741 gcaatacggg cctgcaaaac aatctggcgc tattgctgtt tagtagcgat cgccgtgacg
     7801 aaggttttgc cgtcctggaa cagatggcaa aatcgaacgc cgggcgcgaa ggggcctcta
     7861 aaatctggta cgggcagatt aaagacatgc ccgtcagtga tgccagtgtg tcggcgctga
     7921 aaaaatatct ctcgatcttt agtgatggcg atagcgtggc ggctgcgcaa tcgcaactgg
     7981 cagaacagca aaaacagctg gccgatcctg ctttccgcgc tcgtgcgcaa ggtttagcgg
     8041 cggtggactc tggtatggcg ggtaaagcca ttcccgaact acaacaggcg gtgcgggcga
     8101 acccgaaaga cagtgaagct ctgggggcgc tgggccaggc gtattctcag aaaggcgatc
     8161 gcgccaatgc agtggcgaat ctggaaaaag ccctcgcact ggacccgcac agcagcaaca
     8221 acgacaaatg gaacagtctg ctgaaagtaa accgctactg gctggcgatc cagcagggcg
     8281 atgctgcgct gaaagccaat aatcctgacc gggcagaacg cctgttccag caggcgcgta
     8341 atgtcgataa caccgacagt tatgcagtgc tggggctggg cgatgtggcg atggcgcgaa
     8401 aagattatcc cgccgccgaa cgttattatc agcagacctt gcgtatggac agcggcaaca
     8461 ctaacgccgt gcgcgggctg gcaaatattt accgccagca atcgccagaa aaagctgaag
     8521 cgtttatcgc ctcgctctct gccagtcagc ggcgtagcat tgatgatatc gaacgcagcc
     8581 tgcaaaacga ccgtctggca cagcaggcag aggcactgga aaaccagggc aaatgggcgc
     8641 aggcggcagc acttcagcgg caacgactgg cgctggaccc cggcagcgta tggattactt
     8701 accgactttc gcaggatctc tggcaggccg gacaacgcag ccaggccgat acgttaatgc
     8761 gcaatctggc gcagcagaag tcgaacgacc cagagcaggt ttacgcttac gggctgtacc
     8821 tacgcttacg ggctgtacct ctctggtcat gaccaggaca gagcggcgct ggcgcatatc
     8881 aacagcctgc cgcgtgcgca gtggaacagc aatattcagg agctggttaa tcgactgcaa
     8941 agcgatcagg tgctggaaac cgctaaccgc ctgcgagaaa gcggcaaaga ggcagaagcg
     9001 gaagcgatgc tgcgccagca accaccttcc acgcgtattg acctcacgct ggctgactgg
     9061 gcgcaacaac gacgtgatta caccgccgcc cgcgctgcat atcagaatgt cctgacgcgg
     9121 gagccagcta acgccgacgc cattcttggt ctgacggaag tggatattgc tgccggtgac
     9181 aaagcggcgg cacgtagcca gctggcgaaa ctgcccgcta ccgataacgc ctcgctgaac
     9241 acacagcggc gcgtggcgct ggcacaggcg cagcttggcg ataccgcagc agcgcagcgg
     9301 acgtttaata agttgatccc gcaggcaaaa tctcagccac cgtcgatgga aagcgcgatg
     9361 gtgctgcgtg atggtgcgaa gtttgaagcg caggcgggcg atccaacgca ggcgctggaa
     9421 acctacaaag acgccatggt cgcatccggt gtgactacga cgcgtccgca ggataacgac
     9481 acctttaccc gactgacccg taacgacgag aaagatgact ggctgaaacg tggcgtgcgc
     9541 agcgatgcgg cggacctcta tcgccagcag gatcttaacg tcacccttga gcacgattac
     9601 tggggttcga gcggcaccgg tggttactcc gatctgaaag cgcacactac catgttgcag
     9661 gtggatgcgc cgtattctga cgggcggatg ttctttcgca gtgatttcgt caatatgaac
     9721 gtcggcagtt tctccactaa tgccgatggc aaatgggatg acaactgggg cacctgtaca
     9781 ttacaggact gtagcggcaa ccgcagccag tcggattccg gtgccagcgt ggcggtcggc
     9841 tggcgaaatg acgtctggag ctgggatatc ggtaccacgc cgatgggctt caacgtggtg
     9901 gatgtggtcg gcggcatcag ttacagcgat gatatcgggc cgctgggtta caccgttaac
     9961 gcccaccgtc ggcccatctc cagttctttg ctggcctttg gtgggcaaaa agactccccg
    10021 agcaataccg ggaaaaaatg gggtggcgta cgtgccgacg gtgtggggct aagtctgagc
    10081 tacgataaag gtgaagcaaa cggcgtctgg gcatcgctta gtggcgacca gttaaccggt
    10141 aaaaatgtcg aagataactg gcgcgtgcgc tggatgacgg gctattacta taaggtcatt
    10201 aaccagaaca atcgccgcgt cacaatcggc ctgaacaaca tgatctggca ttacgacaaa
    10261 gatctgagtg gctactcact cggtcagggc ggttactaca gtccgcagga atacctgtcg
    10321 tttgccatac cggtgatgtg gcgggagcgc acggaaaact ggtcgtggga gctgggggcg
    10381 tctggctcgt ggtcgcattc acgcaccaaa accatgccgc gttatccgct gatgaatctg
    10441 atcccgaccg actggcagga agaagctgcg cggcaatcca acgatggcgg cagcagtcag
    10501 ggcttcggct acacggcgcg ggcattactt gaacgacgtg ttacttccaa ctggtttgtt
    10561 ggcacggcaa ttgatatcca gcaggcgaaa gattacgcac ccagccattt cctgctctac
    10621 gtacgttatt ccgccgccgg atggcagggt gacatggatt taccgccgca gccgctgata
    10681 ccttacgccg actggtaagt tttcagatag cgcctctctt aatgccgctg cgatcgggta
    10741 tactcgggcg gcaatctggg atttccgggg ggagacaatt tgcgcgtaag tcgctcgtta
    10801 acaatcaagc agatggcaat ggtggcagcc gttgtcctgg tgttcgtttt tattttttgc
    10861 accgttttgc tgttccatct ggtccagcag aatcgctata acacggctac gcaactggaa
    10921 agcattgctc gctctgtccg cgaaccctta tcttcagcta ttttgaaagg cgatattccc
    10981 gaagcggaag ctattcttgc cagcattaaa ccggcaggcg tggtcagccg tgccgatgta
    11041 gtgctgccta accagttcca ggcgctgcgt aaaagtttta ttccagagcg cccggtgccg
    11101 ataatggtta ctcgcctgtt tgagctaccg gttcaaatct cgctgggcgt ttactcgctc
    11161 gaacgtccgg caaacccgca gccaattgcc tatctggtac tacaggcgga ttccttccgt
    11221 atgtataagt tcgtgatgag caccctctca acgttagtga ccatttactt acttttgtcg
    11281 cttatcctga ccgtcgccat cagctggtgc attaaccgcc tgattttgca tccgttacgc
    11341 aatattgctc gcgaacttaa cgccatccca gcccaggagc ttgttggtca ccaactggca
    11401 ttaccgcgtc tgcatcagga cgatgaaatc ggtatgttgg tgcgcagtta caacctcaac
    11461 cagcaattgc tgcagcgcca ttatgaagaa cagaacgaaa atgcgatgcg cttcccggtg
    11521 tcggatttgc cgaacaaagc cttgctgatg gagatgctgg agcaggttgt cgcgcgtaaa
    11581 caaaccaccg cgctgatgat catcacctgt gaaaccctgc gtgatactgc gggcgtgctg
    11641 aaagaggcgc aacgagaaat tctgctgctg acgctggtgg aaaaactcaa atcggtactg
    11701 tcgccacgta tgatcctcgc gcagattagc ggttatgact ttgctgtcat tgccaacggt
    11761 gtacaggaac cgtggcacgc aatcacctta ggtcagcaag tgctcaccat catgagcgag
    11821 cgcctgccga ttgaacgtat tcaactccgt ccgcactgta gcattggcgt ggcgatgttc
    11881 tacggcgatc tcaccgccga acagctttac agtcgcgcta tttctgcggc atttaccgct
    11941 cgccataaag gcaagaatca gattcagttc tttgatccgc agcagatgga agccgcccag
    12001 aagcggttga cggaagagag cgatatcctt aatgcactgg aaaatcatca gtttgctatt
    12061 tggttacagc cacaggtcga gatgaccagc ggtaaactgg tcagtgcgga agtgttactg
    12121 cgtatccagc aaccggatgg cagttgggac ctgccggatg gcttaatcga tcgcattgag
    12181 tgctgtgggc tgatggttac cgtcggtcac tgggtgctgg aagagtcctg tcgattgctt
    12241 gcagcctggc aagagcgcgg cattatgctg cccttgtcgg taaacctctc tgcgctgcaa
    12301 ctgatgcacc caaatatggt ggcggatatg ctggaactgt taacccgcta tcgcattcag
    12361 ccgggaacac tgattctgga agtgacagaa agccgacgta ttgacgaccc tcatgctgcg
    12421 gtggcaatcc tccgtccgct gcgcaatgcc ggagttcggg tggcgctgga tgatttcggc
    12481 atgggctacg cagggctgcg tcagctgcag catatgaaat cgttgccaat cgacgtactg
    12541 aaaatcgaca aaatgtttgt tgaaggcttg ccggaagata gcagcatgat tgctgcaatt
    12601 atcatgctgg cgcagagcct gaacttacaa atgattgccg aaggcgtgga gactgaagca
    12661 caacgcgact ggctggcaaa agcgggcgtt ggtattgccc agggcttcct ttttgctcac
    12721 ccactcccta ttgaaatctt cgaagagagt tacctggaag aaaagtagct accccaaact
    12781 gattacaaaa ctttaaaaag tgctggtttg tgcgagccag ctcaaacttt ttaacctttt
    12841 tgtttcaatt atgatccagg tacatttctg tgatgttgtc tgggtgttat tttaaggccg
    12901 caggtacccc ataaccttac aagacctgtg gttttactaa aggacaccct atgaaaacct
    12961 ctctgtttaa aagcctttac tttcaggtcc tgacagcgat agccattggt attctccttg
    13021 gccatttcta tcctgaaata ggcgagcaaa tgaaaccgct tggcgacggc ttcgttaagc
    13081 tcattaagat gatcatcgct cctgtcatct tttgtaccgt cgtaacgggc attgcgggca
    13141 tggaaagcat gaaggcggtc ggtcgtaccg gcgcagtcgc actgctttac tttgaaattg
    13201 tcagtaccat cgcgctgatt attggtctta tcatcgttaa cgtcgtgcag cctggtgccg
    13261 gaatgaacgt cgatccggca acgcttgatg cgaaagcggt agcggtttac gccgatcagg
    13321 cgaaagacca gggcattgtc gccttcatta tggatgtcat cccggcgagc gtcattggcg
    13381 catttgccag cggtaacatt ctgcaggtgc tgctgtttgc cgtactgttt ggttttgcgc
    13441 tccaccgtct gggcagcaaa ggccaactga tttttaacgt catcgaaagt ttctcgcagg
    13501 tcatcttcgg catcatcaat atgatcatgc gtctggcacc tattggtgcg ttcggggcaa
    13561 tggcgtttac catcggtaaa tacggcgtcg gcacactggt gcaactgggg cagctgatta
    13621 tctgtttcta tattacctgt atcctgtttg tggtgctggt attgggttca atcgctaaag
    13681 cgactggttt cagtatcttc aaatttatcc gctacatccg tgaagaactg ctgattgtac
    13741 tggggacttc atcttccgag tcggcgctgc cgcgtatgct cgacaagatg gagaaactcg
    13801 gctgccgcaa atcggtggtg gggctggtca tcccgacagg ctactcgttt aaccttgatg
    13861 gcacatcgat atacctgaca atggcggcgg tgtttatcgc ccaggccact aacagtcaga
    13921 tggatatcgt ccaccaaatc acgctgttaa tcgtgttgct gctttcttct aaaggggcgg
    13981 caggggtaac gggtagtggc tttatcgtgc tggcggcgac gctctctgcg gtgggccatt
    14041 tgccggtagc gggtctggcg ctgatcctcg gtatcgaccg ctttatgtca gaagctcgtg
    14101 cgctgactaa cctggtcggt aacggcgtag cgaccattgt cgttgctaag tgggtgaaag
    14161 aactggacca caaaaaactg gacgatgtgc tgaataatcg tgcgccggat ggcaaaacgc
    14221 acgaattatc ctcttaatct caccacttat gcccgcactc ccttccccga gtgcgggtgt
    14281 ttgcgcataa ttgacccctg tgcctgaact taccacctgg gtgagttcag gtttatttca
    14341 cttcttccgt gacattttca tgttcttgcg gtctaacacg aagtgttttt aacgtcattt
    14401 agactgactg acaacgggca gttttttatt accaggatag ttgatcaggg gttcacatgc
    14461 agggcacaaa aattcgactt ttagcgggcg gtttgctgat gatggccact gctggctatg
    14521 tgcaggcaga tgcgctccag cctgatccag catggcaaca ggggacgctt tccaacggtt
    14581 tacagtggca agtgctgacc accccccagc gtcccagcga tcgtgttgaa attcgcctgc
    14641 tggttaatat cggttcgctc gccgaaagta cacaacagag cggttacagt cacgccatcc
    14701 ctcgtattgc gctaacgcaa agcggtggcc ttgacgcagc acaggcgcgt tcattgtggc
    14761 agcaggggat cgaccctaaa cgcccgatgc cgccggtaat tgtctcttat gacaccacgc
    14821 tgtttaatct gagtttgccc aataaccgta acgatttgct gaaagaagcg ctctcttatc
    14881 tggcaaatgc cactggcaaa ttgaccatca caccagaaac catcaaccac gcgctgcaaa
    14941 gtcaggacat ggtggcaacc tggcctgccg atactaaaga gggctggtgg cgctatcgtc
    15001 tgaaagggtc aaccttgtta ggtcacgatc ctgccgatcc gctgaaacaa cccgttgaag
    15061 cggaaaaaat taaagatttc tatcagaaat ggtacacccc ggatgcaatg acgctactgg
    15121 tggtgggaaa cgtggatgcg cgctcggttg tcgaccaaat caacaaaacg tttggcgaac
    15181 tgaaaggcaa acgtgaaacg ccagctccgg tgccgacgct ttctccgctg cgtgcggaag
    15241 cggtgagtat tatgactgac gcggtgcgtc aggaccggtt atctatcatg tgggatacgc
    15301 cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta ctggcgtgcg gacctggccc
    15361 gcgaggcgct gttctggcat gttcagcaag cgttaagcgc cagtaacagc aaagacatcg
    15421 gtcttggatt tgactgccgt gtgctgtatc tgcgtgcgca gtgtgccatc aacatcgaat
    15481 caccaaacga caagctgaac agcaacctta atctggtggc gcgtgaactg gcgaaggttc
    15541 gcgataaagg tctgccggaa gaagagttca atgcgttagt ggcgcaaaag aaactggagc
    15601 tgcagaaact gtttgccgcc tatgcgcggg ctgataccga tattctgatg ggtcagcgga
    15661 tgcgttcgct gcaaaatcag gtcgtcgata tcgcgccgga gcagtatcag aaactgcggc
    15721 aggatttcct taacagcctg acggtggaga tgttaaatca ggatctgcgt cagcagttgt
    15781 cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga gccggaattt aacatgaaag
    15841 cgttgcaggc ggcctgggat caaatcatgg ccccatcgac tgctgctgcc gccacctctg
    15901 tcgccacgga tgacgtacat cctgaagtga cggatattcc acctgcacag taatgcctga
    15961 tggtgatgag ttagcacaac catcaggtat ttatggatga gctggataga ggggtaacac
    16021 tttatccagc cttttgcata tgctgcgttt acgctggcat cgcctcacgc gggataatcg
    16081 cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc gcgtttcgcc gcgttttccg
    16141 cactgccgcc cgtcaaacat accgccagat aaccggcgct gaaagagtcg ccagctgcgg
    16201 tggtatcgat cactttttct ttcggcagtt tcaccgccgg aacatccact aacccttcgc
    16261 cagcaatgga caccaggcaa gaatccgccc cgcgtttcac caccacttct ttcacgcccg
    16321 cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg accccacagc gcgtcttcat
    16381 cgtccagcgt caggaaggcg atatccgtgc attccagcat ttgttggtac acctgctgtg
    16441 tctcttcttt gctggcccac aggcgcggac gatagttatt gtcgaaaatc acttttccgc
    16501 cgttggcgcg gcattcgcgc agcagggaaa gcagcttttc gcggctggtc gggcttaaga
    16561 tcgccaggct aatcccgctc aggtagagat aatcgaaatt cgccagctct tcgcaaatcg
    16621 ccgcagactg ctcactctcc agccagaatt tggcggcggc ttcgttccgc cagtagtaga
    16681 acgtacgctc gccggtgctg tcggtttcaa tgtagtaaag gcccggcaga cggttttcca
    16741 tccgttgggt cagggaagta tcaacgttct cgccgtgcca ggcgtccagc atttgctggc
    16801 taaaactgtc cgttcccagc gccgttacgt aatgaacggt taatgccgca ggatcgacct
    16861 gacgggcgat atagacggaa gtgttcaggg tatcgccgcc gaaaccgcgc ttaacgtccg
    16921 cgcctttctc ggaaagctca atcatgcatt cgccaatcac ggcaatcttt ttggacatag
    16981 tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc tgtggtggtt tgctggtcaa
    17041 ccatattaaa acagtgttcc attatttttt tgagccagaa cgtattcctg aaagattccg
    17101 ttgtggagtg aggaaattta ccccggccag gcggtaaagt tctgcccttg cgcgccgata
    17161 atctttgtcg agtccgggca gcatcacttt taaacacagg acatctttga tgataaggca
    17221 ggttatccag cgaataagca accctgaagc aagcatcgag agcttgcagg aacggcgttt
    17281 ttggttgcag tgtgagcgtg cttacacctg gcagccgatc tatcaaacat gcggacggtt
    17341 aatggccgtg gagctattaa cggtggtcac gcatcccttg aacccttcgc aacgcctgcc
    17401 gccggatcgc tattttactg aaatcaccgt cagccatcgg atggaggttg tgaaagagca
    17461 gattgatttg ctggcgcaaa aagccgactt ctttgtagag cacgacctgc tggcatcggt
    17521 caatattgat ggccctacgc tcatcgccct gcgtcagcaa ccaaaaatcc tgcgccaaat
    17581 tgagcgtctt ccctggctgc gtttcgaact ggtggaacac atccgcctgc cgaaagattc
    17641 aacctttgcc tcgatgtgtg aatttggccc gctgtggctg gatgattttg gcaccgggat
    17701 ggcaaatttc tctgcgctaa gtgaagtgcg ttatgactac atcaaaatcg cgcgagagct
    17761 gttcgtgatg ctgcgtcagt cgccggaagg ccgcacactc ttttctcagc ttttacatct
    17821 aatgaatcgc tattgtcgcg gggtgattgt cgagggcgta gaaacaccgg aagagtggcg
    17881 tgatgttcag aactcgcccg cattcgccgc acaaggctgg tttctttcac gcccggcacc
    17941 gatggaaacg ctgaatacgg cggttctggc gctataagct gcctcatttt ccgctgggct
    18001 ggactatctt taggactggc acaggaagaa tatgaggaag cgaacgatga gcaaggcagg
    18061 caaaataacc gctgcgattt caggggcttt cttgttgttg attgtcgtgg cgatcatttt
    18121 gattgcaact tttgactgga atcgactcaa accgaccatc aaccagaaag tctctgcgga
    18181 gttgaatcgt ccgttcgcta tccgtggcga tctgggcgtg gtgtgggagc ggcaaaaaca
    18241 agaaactggc tggcgcagct gggtgccgtg gcctcatgta catgcagaag acatcattct
    18301 tggtaatcca ccggatattc ccgaagtcac gatggtgcat ttgccgcgag tagaggcaac
    18361 gctggcaccg ctggcgctgc tgaccaaaac ggtctggctg ccgtggttca agctcgaaaa
    18421 gcccgacgcg cgcctgattc gcctctccga aaagaacaat aactggacgt ttaatcttgc
    18481 caacgatgac aacaaagacg cgaatgcaaa gccgtcggca tggtcgtttc ggctggataa
    18541 tattcttttc gatcaagggc ggatcgccat tgatgacaaa gtaagcaaag cggatctgga
    18601 aatttttgtt gatccgttag gcaagccgct gccgttcagc gaagttactg gatcgaaagg
    18661 taaagcggat aaagaaaagg tgggcgatta cgtttttggc ctgaaggcgc agggacgtta
    18721 taacggtgaa ccgctcacgg gtacgggaaa aataggcggt atgctggcgc tgcgtggcga
    18781 agggacgccg tttccggtac aggctgattt ccgttcaggt aatacccgtg ttgcttttga
    18841 tggcgtcgtg aatgacccaa tgaagatggg cggtgtcgat ttacggctta aattttctgg
    18901 cgattcactg ggtgatctct atgaactgac gggcgttctg ctgcccgata ccccgccgtt
    18961 tgaaacggat ggtcggctgg tagcgaaaat cgacactgaa aaatcgtcgg tctttgatta
    19021 tcgcggtttt aatgggcgaa ttggcgatag cgatatccac ggttctctgg tctacaccac
    19081 cggaaagcca cgaccaaaac tggaaggtga tgtcgagtcg cggcaattgc ggctggcgga
    19141 cctgggaccg ttgattggcg ttgattccgg gaaaggggca gaaaagtcga aacggtctga
    19201 acagaagaag ggcgaaaaaa gcgttcagcc tgcgggcaaa gtgctgcctt atgaccgctt
    19261 cgaaaccgat aaatgggacg ttatggatgc cgatgttcgc ttcaaagggc ggcgcattga
    19321 gcatggcagt agcctgccga ttagcgatct ttctactcat atcatcctca aaaatgctga
    19381 cctgcgcctg caaccgctga aatttggcat ggcgggcggc agcattgcga cgaatattca
    19441 tctggaaggc gataaaaagc cgatgcaggg gcgggcagat attcaggctc gtcgactgaa
    19501 actgaaagaa ctgatgcccg atgtggaact gatgcagaag acgctggggg aaatgaacgg
    19561 tgacgcggaa ctacgcggta gcggtaactc ggtggcggcg cttttaggca acagtaacgg
    19621 caacctgaaa ctgttgatga atgacgggct ggtgagccgc aacctgatgg agattgttgg
    19681 gctgaatgtc ggcaactaca ttgtcggtgc gatatttggt gacgatgagg tgcgggtgaa
    19741 ctgcgcggcg gcgaatctga atattgccaa cggcgtggcg cgcccgcaga tttttgcttt
    19801 cgatactgag aacgcgttga ttaatgttac cggcacggca agttttgctt cagaacagct
    19861 ggatttgact attgatccgg agagtaaagg gattcggatt atcacactgc gttcgccgct
    19921 gtatgtgcgt gggacgttta aaaatccgca ggctggggtg aaagccggac cgctgattgc
    19981 ccgtggtgct gttgctgcgg cactggcaac gctggtaaca ccgacggcgg cgttactggc
    20041 actgatctca ccttccgaag gggaggctaa tcagtgtcgg acgatattgt cgcagatgaa
    20101 gaagtgatga taattgccgg atgtctgaca tccggcaatt aaacagattt agatttttgg
    20161 caaagtgtgc tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaatcct
    20221 tgtgaattca atatattgta tggaaattca gtcctgataa gcgtagcgca tcaggctttt
    20281 cacacttatt acaacgactg atgtcgcgtc tcatgggtca gcagcagggc gattaacgtc
    20341 aagccagcca tcgccgccag atataacccc accgcaccta acccgtagtt agtctgcaac
    20401 caggctgcga tatacggtgc aacggaagcc ccgagaatcg acgctacgtt gtaagagaat
    20461 gatgctccgg tgtaacgcac ttctgttggg aacagctctg gtaacagcgc gcccatcggc
    20521 ccgaaggtca gccccatcag acttaacccc agcagcagga aggcaaaaac cagaatcggg
    20581 ttgccagaac cgagcagcgg gttaaaggcg aacagcgcga acaggatgat cagcgtggtg
    20641 ataattacca tgcttttacg gcgaccaaag gcatcagcca gtaatccggc gactggcacc
    20701 atcacgccaa aaccaataac tgccatcatc agcatccaca acacttcgtt acgcggcagg
    20761 ccaagcccaa ctggcgcggc ggcggtactg aaggtcatag agtagaccgt catgatgtaa
    20821 aacagcgtat aggttgccag catgatgaac gtacccagta cggttacgcg aacatgtttg
    20881 gtcagcagcg tacccagcgg gatcttcacc tgttttttcg ctttagcgac tttctcaaac
    20941 accggcgact catgcagcga cacgcgaaca tacaggccga taatgaccag taccgccgag
    21001 aagataaatg gcacacgcca gccccagctc ataaactgct cgtcggtcag tagccaggaa
    21061 agcagcaaga aagtgccgtt ggcaaagaag aagccaatcg gtgcgcccag ctgtgggaag
    21121 gagccataca gtgcacgttt gcgcggtggg gcgttttcag tcgccagcag cgccgcgccg
    21181 ccccattcac cgcctaagcc cagaccctga ccaaatcgag ccagcgccag cagcagcggg
    21241 gcgaaaatac caatcgtggc atagcccggc agcagaccaa tcaccacggt tgaaatcccc
    21301 atcgttagca acgaggcgac cagcgtcgct ttacgcccaa cgcgatcgcc aaaatgacca
    21361 aaaacggcag agccaatggg gcgcgcgacg aaggcgatgg cgaaggtggc gagcgactgt
    21421 agcgttgctg ccgttggatc gccctgcggg aagaagatat gcggaaacac aataacggcc
    21481 gcagtggcgt aaatgtaaaa gtcgaagaac tcaatggccg tgccaatgag agaggcgaca
    21541 aggactttat tacgcgagtt gatcggcgtg tattcttgct cgtggtcgag tgttgtggct
    21601 gttgcttgca taatcttttc ttattttttg gctaacgaat agccatatta cgcacagcaa
    21661 aagtgatatt tcaatgtgta acaaatcgcg gaaatggtca aaaaccaggc aaaaagggga
    21721 taatgtttag gccagtgatt tttcttctat gaaatgcttc acataatttt ataaatagtg
    21781 gataaaactg gtttcaggtt aaataaaagt taccaactgg atttatatgc tggggttatg
    21841 agttggaatg aaattgtttg ctcccaaata gatggctgaa gaggctctgg ggaagagttt
    21901 caagaggtta tgtgcgcatt atagccggat gcgatgctgg cgcatcttat ccggcctacg
    21961 gggagccagg ccgaataaaa tcgtatgccg gataaggcgt ttacgccgca tccggcctat
    22021 tttaaggctg cgttttccct ggcattcgcg ggtcgtcttt atattcggcg gtggcaatcc
    22081 acgccgcgca aaacagcgtc aaacgggcga agaagtagaa aaatgccatc agccccagca
    22141 cggaaccaaa tgctgcgcca gagggggatt tcatcagcga tggcagggtg taggtcatca
    22201 cgattttaat cacttcaaaa ccaatagcag ccagaaatgt tccgcgaatc agggcttttt
    22261 tgcgcggacg gtggcgcggt aaccgccaga agatccagaa gaaaagcaga tagttggcga
    22321 agatggaaat cgccaggcca atcaaccgcc acgtcggttt cagccactca atgctgttca
    22381 ggtgcagggc gctaataatc atttgctgcg ccgaaccggc aaccgaggtg atcgaaagcg
    22441 tcacaatcag cgcaatcaac aaaccaatca gcgaaataaa atcacgtaga tattttaccc
    22501 agaacttctc ctgatcttgc ggcgagcgtt cccagacatc gcgcgactgg gcgcgaatcg
    22561 cttcacgcag gttacccatc cagttgatgc cggaataaag cgccacagcc aggccgacaa
    22621 gccctacagt cgtacgctgc tgaacggcgg tgttgatggt gtttttcaac gtggcggcta
    22681 gcgtcggatc gctgatgttt tgcagaattt tgtcgaagat atcctgtagc aacatcggat
    22741 gggaggccag cacaaagccc cctgcggcaa acgacaccat caaaatcggg atcatcgaca
    22801 aaaatgagaa ataggtgata gccgcaccaa actggttacc cagccgatcg ttaaagcgtt
    22861 ctgtcgcgcg aatcaggtgc gcaataaccg gttggcgttg gactttttcg gcagtggtgg
    22921 tgacggtttc cagcgcctgg ctaactttgc cgcctttctc gctattatcc aactgcttaa
    22981 tcggctcgtg ctccagatcc tgggtgggac gtttgatctc gttttcctgc gtcatccgct
    23041 cttttgtcct tctgttgtgg tcattcttaa aaagtatagt cggtcagtcc aggtactctt
    23101 tcattacgcc agccagccac tgcataaaca ggtttacacg ccgggaaagc tcccgacgct
    23161 gcggataaac cagcgaaagg gagagcggct cggcacggta gcctggcaac acttcaataa
    23221 gctgcccggc acgcagggct tcgcgcacgg caatgcgcgg gatctgaata atccccagac
    23281 cggcaagacc ggcggcgaga tagttttcgc tactgtttac cgtcaacatg ccgccagact
    23341 taaaccactg gacgccatta acgctggcaa cctcaaaacc taacggatgt acacccaggt
    23401 gcggtgtata acgcaccatt gcgtgtgaag tcagatcatc ggggctttgc ggataaccaa
    23461 agcgcgtcag atagtgcgga ctggcacagt tgaccatggt cagtttgccg aggggacggg
    23521 cgataacgcc atcttccggt aacgcgccag tgcgtatcac gcaatcaaaa ccatcatgaa
    23581 gaatatctac cggacggtta tggctactca gttccagctc aattcccgga tgctgataga
    23641 gaaattccga cagacgcggc agtaacaggt ttttcgcgat tcctgacggg atgtcgatgc
    23701 gtaatttacc gctgatactg gtggcatcct gttgaaacag accgtccagt tcgctgagat
    23761 tactcaacac atcttttgcc cgttgataat aggtcatgcc ttctggcgtg agtttgaccc
    23821 gtcgcgtagt gcgctggaga agctgggtgc caagttgatg ttccagtgcc tgtatctggc
    23881 gcgaaacact tccctttggc aaagcaaaga aatccgccgc gcgggaaaaa ctttccagct
    23941 ccgcgacttt gatgaacaac tgcattgcgt gaattttatc catttctttg accgattgtt
    24001 gttcacagcg aaacagtaac gcgcatatcg cattgtttat tgttttttct aacatctaat
    24061 gccgggtttc acaatgttgt catgacgaat gtggcgattt ctcagcagga aaagggcgga
    24121 atagctttat gagaaaggtg aatcccctta aacagagcgt atttttcagt gattcccttt
    24181 aaagtgaata gaggacgtat tggctatttg tgcttatttt tgtctcattg ccatttgtta
    24241 ttttggcttg tagtcgcgga atttcatttt taattgcatg cgtactaacc accaatataa
    24301 ggaaatcctc aaaattgttt taaatagtgc cctcgacatg taatttcagg cgtttagctg
    24361 taactattta tatcggtaac tattttctac gcgtcatctc tgtagaatgg ccaccgccaa
    24421 atggcgaatt cttatttttt ctcgcgggta atacccttcc actacttaag tctttttaac
    24481 gtattcaggt ggagattcag ttatgcaaat agtcatgttt gacaggcagt caatatttat
    24541 tcatggaatg aaaatcagtt tacagcagcg tattccagga gtgagtattc agggggccag
    24601 tcaggcagac gagttatggc aaaagctgga aagttaccct gaagccttag ttatgctcga
    24661 tggcgatcag gatggtgagt tttgctactg gttgctgcaa aaaaccgtgg tgcagtttcc
    24721 tgaggttaag gtgttaatca cggcgacgga ttgcaataaa cggtggttac aggaagttat
    24781 tcattttaat gtgctggcca ttgtgcctcg tgattcaacc gtcgagacat ttgcgttggc
    24841 ggtaaatagt gcggcgatgg ggatgatgtt tctgcctgga gactggcgga cgacgccgga
    24901 aaaggacatt aaagatctca aatcgttaag cgcccgccag cgggagattt taaccatgtt
    24961 agcggcgggc gaatcaaata aggagatcgg cagagcgctg aatatcagta ccggaacggt
    25021 aaaagcgcat ctggaatccc tgtatcgccg tctggaagtg aaaaatcgca cccaggcggc
    25081 aatgatgtta aatatctcct cctg
//
LOCUS       DAJVQG010000050        24961 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJVQG010000050 DAJVQG010000000
VERSION     DAJVQG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.050"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..1565)
                     /gene="pdeC"
                     /locus_tag="OQM83_001816"
     CDS             complement(<1..1565)
                     /gene="pdeC"
                     /locus_tag="OQM83_001816"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCS1520097.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPAR"
     gene            1995..2275
                     /locus_tag="OQM83_001817"
                     /pseudo
     CDS             1995..2275
                     /locus_tag="OQM83_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2374..2910)
                     /gene="ssb1"
                     /locus_tag="OQM83_001818"
     CDS             complement(2374..2910)
                     /gene="ssb1"
                     /locus_tag="OQM83_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCS1520098.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3164..5986
                     /gene="uvrA"
                     /locus_tag="OQM83_001819"
     CDS             3164..5986
                     /gene="uvrA"
                     /locus_tag="OQM83_001819"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCS1520099.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6021..6377)
                     /locus_tag="OQM83_001820"
     CDS             complement(6021..6377)
                     /locus_tag="OQM83_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCS1520100.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6381..6797)
                     /locus_tag="OQM83_001821"
     CDS             complement(6381..6797)
                     /locus_tag="OQM83_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCS1520101.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(6908..7621)
                     /gene="aphA"
                     /locus_tag="OQM83_001822"
     CDS             complement(6908..7621)
                     /gene="aphA"
                     /locus_tag="OQM83_001822"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCS1520102.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            8023..8245
                     /locus_tag="OQM83_001823"
                     /pseudo
     CDS             8023..8245
                     /locus_tag="OQM83_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8749..9942)
                     /gene="tyrB"
                     /locus_tag="OQM83_001824"
     CDS             complement(8749..9942)
                     /gene="tyrB"
                     /locus_tag="OQM83_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCS1520103.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(10195..11274)
                     /gene="alr"
                     /locus_tag="OQM83_001825"
     CDS             complement(10195..11274)
                     /gene="alr"
                     /locus_tag="OQM83_001825"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCS1520104.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(11327..12742)
                     /gene="dnaB"
                     /locus_tag="OQM83_001826"
     CDS             complement(11327..12742)
                     /gene="dnaB"
                     /locus_tag="OQM83_001826"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCS1520105.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            12825..13808
                     /locus_tag="OQM83_001827"
     CDS             12825..13808
                     /locus_tag="OQM83_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCS1520106.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(13974..14216)
                     /gene="pspG"
                     /locus_tag="OQM83_001828"
     CDS             complement(13974..14216)
                     /gene="pspG"
                     /locus_tag="OQM83_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCS1520107.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(14350..15387)
                     /gene="dusA"
                     /locus_tag="OQM83_001829"
     CDS             complement(14350..15387)
                     /gene="dusA"
                     /locus_tag="OQM83_001829"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCS1520108.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(15750..16735)
                     /locus_tag="OQM83_001830"
                     /pseudo
     CDS             complement(15750..16735)
                     /locus_tag="OQM83_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            17053..17568
                     /gene="zur"
                     /locus_tag="OQM83_001831"
     CDS             17053..17568
                     /gene="zur"
                     /locus_tag="OQM83_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCS1520109.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(17610..17819)
                     /locus_tag="OQM83_001832"
     CDS             complement(17610..17819)
                     /locus_tag="OQM83_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCS1520110.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(17935..19260)
                     /gene="dinF"
                     /locus_tag="OQM83_001833"
     CDS             complement(17935..19260)
                     /gene="dinF"
                     /locus_tag="OQM83_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCS1520111.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(19333..19941)
                     /gene="lexA"
                     /locus_tag="OQM83_001834"
     CDS             complement(19333..19941)
                     /gene="lexA"
                     /locus_tag="OQM83_001834"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCS1520112.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(20051..20419)
                     /gene="dgkA"
                     /locus_tag="OQM83_001835"
     CDS             complement(20051..20419)
                     /gene="dgkA"
                     /locus_tag="OQM83_001835"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCS1520113.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            20590..23013
                     /gene="plsB"
                     /locus_tag="OQM83_001836"
     CDS             20590..23013
                     /gene="plsB"
                     /locus_tag="OQM83_001836"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCS1520114.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(23168..24040)
                     /gene="ubiA"
                     /locus_tag="OQM83_001837"
     CDS             complement(23168..24040)
                     /gene="ubiA"
                     /locus_tag="OQM83_001837"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCS1520115.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(24053..24550)
                     /gene="ubiC"
                     /locus_tag="OQM83_001838"
     CDS             complement(24053..24550)
                     /gene="ubiC"
                     /locus_tag="OQM83_001838"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCS1520116.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(24773..>24961)
                     /locus_tag="OQM83_001839"
     CDS             complement(24773..>24961)
                     /locus_tag="OQM83_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1520117.1"
                     /translation="DMLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYAL
                     FPIFTASWRATRIKNIGI"
BASE COUNT         6463 a         6422 c         5986 g         6090 t
ORIGIN      
        1 cgcgcgggta attgctccat ccattgcata aacacctgcg gcggcatcgc cttcgcaaaa
       61 aaccatccct ggcaatagcg cacgccgcgt ttgcgcagcc agttaacctg ctcctcagtt
      121 tcgacgcctt cagcgatcgt ttttaacccc aggctgtgcg ccagctcgat gatgtgttcc
      181 gcaatcaaat gactggtttt gtgggtggtc agcgtttcaa caaacgattt atcgattttc
      241 aaaatatcga cattcaatga tttaaggtta tgcaagttag agtagccaat accaaaatca
      301 tcaattgcca cttcgtaacc agcctggcgg aaagccagaa taatcggcgt cattttttcg
      361 acatcaagaa atgcatgctc agtcacttca aatttaattt gctgcggacg caccgcgtat
      421 tgctctgttt tctgattgat tcgcgctatc aaccgtgacg tatgaaaatc ggaggccgac
      481 aggttaatag aaacatagcg atcggcatgt gttgccaagt acgcgcccag atcgcggaag
      541 acattatcaa taacataatc agttatctgt tctatcatcc cctccttttc tgccagcgga
      601 ataaactctg ccggattcat tatttgcccc tgctcaccaa gccaacgtaa caacgcttca
      661 gcgccgatac atttttctgt tttgatatcg attattggct ggtaataaag acaaagttga
      721 tgtttttcga gggcgcgttg caatttacgt ttgggagata aatagttttg tcgaatacgt
      781 agccagagta atagcagaac aagactcccc aaaataccgg cgggcaacgc aaaaataaga
      841 tgattataaa aatgggttat aagacgttga tatgaagtcg caacaatggc tgcaattggg
      901 cgttttgtcg aataaactgt cgcatataaa tagccatttc tttgtacggt taaatccttc
      961 aaatgaatca gcggagaaaa cgttgctgcc gaggcctctt tgcttaacga gaaaaaggtt
     1021 ttcgtcaccg tatcatacac accccattgc aatgtcgggt catcagacat cacttcactc
     1081 cagaagagag ggttgataac cgccacataa tttccccgct gcatataggt cattttatag
     1141 ccagagaaaa aaggcgtatc gcggtaataa tagatagaaa cgttaggttc acgcttataa
     1201 tcggccggtg caatcgtata gccgtttaca ggcgctatca gcgatgagca taaaaaatgg
     1261 ttatcacggg catagatcaa ttcattaata taaagatagc cacgaataat attcaacatt
     1321 cgcttttgat gggctggagt gcaaacttgc ccctgatagc gttcagctgc atcgctcact
     1381 aaatctgcct ggcgaatgac cagctcggat ttatccagtg ccagttgagc aaaggttcgg
     1441 agctgattat tcacttctga ttttgcccaa aggaaggcaa tccatagcga aagaatgatg
     1501 ggaaagagaa ctaaaaagat aatgcccggc aacgccagta attggtgtcg tgcacgatga
     1561 ctcattgcta tcaatttcct gcctgtttat acccggccat tgcaaggtga aaaccctggg
     1621 taccgacgtc tttagataaa tcttacggta taaatagcac ttttgcctga tttgaaattg
     1681 atacctttct aatcgtcacc tggatgaaaa taattcattg atattcaaaa ttaatttcct
     1741 acaggaatgc agaggcggcg ggaagatatg cccttaatgc cactgaaaaa ccacataatc
     1801 tgtacagcaa taaaagtcac ggcctaatat ggctaacaaa caatcaattg tgatatagct
     1861 cacaaaatta atgaaacaaa cagagtgttt catttttgtg ttctgcaagc cccgcgatag
     1921 ccagagtgta ccacgcctcc cgtgaacaac gccgcgctgt ccagggcatc ggctcttttc
     1981 agaggaattg tttgatggct accctcacca ctggcgtggt tcttcttcgc tggcagcttc
     2041 ttagtgccgt aatgatgttt ctggccagca catcaacatc cgttttcgtc ggtctgatta
     2101 tgtcgggctt gcggtgatca gcagcggtct gggcgttgtt tccgcctgtt ggttcgcaat
     2161 ggggttgctt ggcatcacaa tggcggacat caccgctatc tggcacaaca tcgagtcggt
     2221 gatgatagaa gagatgaatc agacaccgcc acaatggcca atgattttga cctgatacgt
     2281 agaagcctcc ataacggagg cttctttttt atggctggca gatgctttaa tcatccacct
     2341 taaaacaata taacctattg ttttaatgac aaatcagaac ggaatgtcat catcaaagtc
     2401 catcggcggc tcgttagacg gcgctgccgg agcggactgc tgcgggcgag actgcgcgcc
     2461 gccgctgaac tgattgccac cctgcggctg ctgaggctgc ccccaaccgc cctgcggctg
     2521 accaccaccg atattaccac ctgccggagc gccaccaccc tgacgaccac ccagcatctg
     2581 catggtgccg ccaacgttca ccacgacttc tgtggtgtag cgatcctgac cggattgatc
     2641 ggtccattta cgggtacgca gctgaccttc gatataaacc tgagaacctt tacgcagata
     2701 ttcgctggcc acttctgcca gtttgccgaa cagcacaacg cggtgccatt cagtctgttc
     2761 tttcatctcg ccggtcgctt tatcacgcca ggattcggaa gtagccagcg taatgttggc
     2821 aactgcgcca ccatttggca tgtagcgtac ttccgggtcc tgacccagat taccaacgag
     2881 aataaccttg tttacgcctc tgctggccat gttcgtgtct cctgaaaaaa atcgttctga
     2941 ataagtgtaa acgcgcgatt gtaccattac caatagcgct tttactatgt tgtgacctcg
     3001 gttccgggaa acaaacctgg ccagacattg ttacacaaca ctccggataa tgcattccaa
     3061 tactgtatat tcattcaggt caatttgtgt cataattaac cgtttgtgat cgccggtagc
     3121 accatgccac cgggcaaaaa agcgtttaat ccgggaaagg tgaatggata agatcgaagt
     3181 tcggggcgcc cgcacccata atctcaaaaa catcaacctc gttatccccc gcgacaagct
     3241 cattgtcgtg accgggcttt cgggttctgg caaatcctcg ctcgctttcg acaccttata
     3301 tgccgaaggg cagcgccgtt acgttgaatc cctttccgcc tacgcgcggc agtttctgtc
     3361 actgatggaa aagccggacg tcgatcatat tgaggggctt tctcctgcca tctcaattga
     3421 gcagaaatcg acgtctcata acccgcgttc tacggtgggg acaatcaccg aaatccacga
     3481 ctatttgcgt ttgttgttcg cccgcgtcgg cgaaccgcgc tgtccggacc acgacgtccc
     3541 gctggcggcg caaaccgtca gccagatggt ggataacgtg ctgtcgcagc cggaaggcaa
     3601 gcgtctgatg ctgctcgcgc caatcattaa agagcgcaaa ggcgaacaca ccaaaacgct
     3661 ggagaacctg gcaagccagg gttacatccg tgctcgtatt gatggcgaag tctgcgatct
     3721 ttccgatccg ccgaaactgg aactgcaaaa gaaacacact attgaagtgg tggttgatcg
     3781 cttcaaggtg cgtgacgatc ttacccaacg tcttgcggag tcgtttgaaa ccgcgctgga
     3841 gctttccggt ggtacagcgg tagtggcgga tatggacgac ccgaaagcgg aagagctgct
     3901 gttctccgcc aacttcgcct gcccaatttg cggctacagt atgcgtgaac tggagccgcg
     3961 actgttttcg tttaacaacc cggcaggtgc ctgcccgacc tgtgacggcc ttggcgtaca
     4021 gcaatatttc gatcctgacc gcgtgatcca aaaccccgag ctgtcactgg ctggcggtgc
     4081 gatccgtggc tgggatcgcc gcaacttcta ttatttccag atgctgaaat cgctggcaga
     4141 tcactataag ttcgacgtcg aagcgccgtg gggcagcctg agcgcgaacg tgcataaagt
     4201 ggtgttgtac ggttctggca aagaaaacat tgaattcaaa tacatgaacg atcgtggcga
     4261 tacctccatt cgtcgtcatc cgttcgaagg cgtgctgcac aatatggagc gccgttataa
     4321 agagacggaa tccagtgcgg tacgtgaaga attagccaag tttatcagca atcgcccatg
     4381 cgccagctgc gaaggaacgc gtctgcgtcg ggaagcgcgc cacgtgtatg tcgagaatac
     4441 gccgctgcct gctatctccg acatgagcat cggtcatgcg atggaattct tcaacaatct
     4501 caaactcgca ggtcagcggg cgaagattgc agaaaaaatc cttaaagaga tcggcgatcg
     4561 tctgaaattc ctcgttaacg tcggcctgaa ttacctgaca ctttcccgct cggcagaaac
     4621 actttccggc ggtgaagccc agcgtatccg tctggcgagc cagattggtg cgggcctggt
     4681 tggcgttatg tatgtactgg acgagccgtc tatcggcctg caccagcgcg ataacgagcg
     4741 cctgttgggt acgcttatcc atctgcgcga tctcggtaat accgtgattg tggtggagca
     4801 tgacgaagac gcaattcgcg ccgctgacca tgtgatcgat atcggtcctg gtgcgggtgt
     4861 tcacggcggt gaagtggtcg cagaaggtcc gctggaagcg attatggcgg tgcctgaatc
     4921 gttgaccggg cagtacatga gcggtaaacg caagattgaa gtgccgaaga aacgcgttcc
     4981 ggcgaatcca gaaaaagtgc tgaagctgac aggtgcacgc ggcaacaacc tgaaggacgt
     5041 gacgctcacg ctgccagtcg gtctgtttac ctgcatcaca ggggtttcag gttccggtaa
     5101 atcgacgctg attaacgaca cactgttccc gattgcccaa cgccagttga atggtgcgac
     5161 catcgccgaa ccggcaccgt atcgcgatat tcaggggctg gagcatttcg ataaagtgat
     5221 cgatatcgac caaagcccaa ttggtcgtac tccacgttct aacccggcga cctataccgg
     5281 cgtgtttaca cctgtgcgcg aactgtttgc gggcgtaccg gaatcccgtg cgcgtggtta
     5341 tacgccagga cgtttcagct ttaacgtccg tggcgggcgc tgcgaagcct gtcagggcga
     5401 cggtgtgatc aaagtggaga tgcacttcct gccggatatc tacgtgccgt gcgatcagtg
     5461 caaaggtaaa cgctataacc gtgaaacgct ggaaattaag tacaaaggca aaaccatcca
     5521 cgaagtgctg gatatgacca tcgaagaggc gcgtgagttc tttgatgcgg tgccagctct
     5581 ggcgcgtaag ctgcaaacgt tgatggacgt tggtctgacg tacattcgcc tggggcagtc
     5641 cgcaaccaca ctttctggtg gtgaagccca gcgcgtgaag ctggcgcgtg agctgtcaaa
     5701 acgcggcacc gggcagacgc tgtatattct cgacgagccg accaccggtc tgcacttcgc
     5761 cgatattcag caactcctcg acgtgctgca taaactgcgc gatcagggca ataccattgt
     5821 ggtgattgag cacaatctcg acgtgatcaa aaccgctgac tggattgtcg acctgggacc
     5881 agaaggcggc agtggcggcg gcgaaatcct cgtctccggt acgccagaaa ccgtcgcgga
     5941 gtgcgaagct tcgcatacgg cacgcttcct caagccgatg ctgtaatcgt taaggccgct
     6001 ttctgagcgg ccttttcctt tcagagttgt accagcaatt tacgcttttc ttccggtagt
     6061 aaattcactg cctgctgata agacgcatcc accagataat agatttgcga atccggcagc
     6121 gaaccgtcga gatacacggt gctccagtgc gctttattca gatggcggct aggacgcaca
     6181 tcgctgtgct gctgacgtag cagctccgcc agctccgggc tggttttcag cgaaacagct
     6241 gggcgatttt ctacttcttt caccatcgca aacagtacat cttccacttt gatctgcgtc
     6301 gctttccagt cgttatgcac gctctgttct gcgcctggtt ttgccatgca atattgtagc
     6361 aactccgaaa tggtcatttt ttactcccct tgtagtgtcg cgatgatgcg acgcgatccg
     6421 ccgtggatgc gatgttcccc cagccagatg ccttgccagg tgccggtctg aatacgccct
     6481 ttatgtaacg gcaatacaag cgatgttccc agcattgagg atttgatatg agaaggcata
     6541 tcgtctgctc cctcatagtc atgctcataa tttccgttgt cgggaacggt gcggaggaaa
     6601 aaacgctcca tgtcgtggcg tacggtggga tcgcagttct cattaagtgt cagagaggcg
     6661 gaggtatgtt gcagcaacag atgcagtaag ccgatgttaa cgcgcggcat atcagccagc
     6721 tgattcagaa tttcatccgt taccagatga aacccacgag atttggcgct aagcgtgagc
     6781 gtcttttgat accacatgtg ctgctccttg ataaaactct cttaatcagt ttgcagcaag
     6841 acagcgaaag gataaaggtg tgattaaaaa aacagcattc gggagagcat cacgctctcc
     6901 cgctctgtca gtattctgaa ttgacgatca cctcttcacc aaacgcaccc gcttgtggca
     6961 agggtttgta ggtagagttg gaggcgcgca gaatgcggat accacgagcg ccgacatcgc
     7021 gtgcggcggt aatatcgtta tcggaatcgc cataaaaaat tcggatattt ttatcctgca
     7081 gccattgcga ttttgtattt tgccctggtt tatcgcccgc aaagattacc ggattcatgt
     7141 tggtggcagg aatatgaaaa ttatccgcca gcgtttttga aaccgtttct gttttcgtcg
     7201 ggctacgacc agtcacaaag aagatcgcgt caccgcggcg tacatgcata tcaatcagct
     7261 ggcgagcgac ctcttttgga atgctgaatt catcccagcc attattcatt ttttcccaga
     7321 acacaggatt tttcagataa tcttcgcttt ctggcgagaa ggtttttttg ccgcgccaga
     7381 accccggact ggaaaaaagt accgtgtcat cgatatcaaa ccccaccgcc attggcggac
     7441 gccctgcgag gctattttca atttgtgcga ccgaaaccca atgaattggt gcctgttcag
     7501 caagcctggc aacgttagtc ccagggttaa gcggtgaagg agatgaggcc agggcaacag
     7561 cggaactgtt tagagcgaac aataagcaaa cggcactgat tgcctgtgtg atcttgcgca
     7621 tatttttccc taaatagtca gtatgttgaa actttttatt gtgagatttg ttgcaaaaac
     7681 catctgacca taacgccagc tgccggggaa aggaaggatt atctgcggtt tttgtgatga
     7741 aaagcaggat gattatattt aacccagtaa atacgttcct gtcttttttt gtataaaaaa
     7801 tgtttattac tcgatgtaag gatacattaa ttttacgttt attatgatga atcctctgca
     7861 aatgagcgaa atttgaaaga acatttatct gctgtacgat ttaatgaact taatgataca
     7921 gcattacaaa aacaatcgaa gtttataaag atgattcctg attgaccaga accctatcct
     7981 acaatggtga ctaattttat tactcccgat ggagatgatg atatgaatat atcatatgtg
     8041 aatagcaaca aaacgacatc tttacctgtt gagctagatg cactaaataa taaagacatc
     8101 tcttatgcca aagatttttc ttatgctaaa gatttctttt tatacataga aacacagtta
     8161 aaaattgcaa aagatttttg tagacctgga gaagaagtat caagttctat tgcaagtaaa
     8221 gtttttcacg catttatcga tttagttaac aaaatcaggg gtaagaaaga ctttatgtat
     8281 atttgcacgc tttgctgttt tgccgaggag gttaaaggtg attattctca ttacaggacc
     8341 tttttatttg atattggtaa tcaatataag gttaaactta cacaaagcgg aaaaaaagag
     8401 ttctctttaa cattagaatt taacgatact ataattgaat ctcagaaagt cacaggcaat
     8461 aaggcaaagt atattcttga ggatatagaa atattctatc gtaacaaacc cgatacttat
     8521 tattaaattt aataatggtt agatgtgcag aaattattac tccacacgaa aaaataggga
     8581 aaggaattcc agataattgg gataaactta acaataaaag aactgacaac gttttattat
     8641 ctctcacgta gaacaacggc atcaaaaaaa tcatctacaa agaaatagtg aaagcgacga
     8701 cagtttaatt tcactgcagg ctgggtaact ccagcctgct ttcctgcatt acatcaccgc
     8761 agcaaacgcc tttgccacac gttgtacatt tgccgtattt aacccggcga cacacatgcg
     8821 accgctggcg atgagataga caccaaattc ttcacgtagt cggtcaacct gagcggcact
     8881 taaaccggta taactgaaca tgccgcgctg attaagcaga taatcgaaat tgcgttctgg
     8941 catctctgtg cttaatacct tcaccaattc ctgacgcatt gccagaatgc gagtacgcat
     9001 ctcttctact tccgccagcc agctggcttt caatgcctcg tcattcagca ctgcagccac
     9061 cacctgcgca ccaaaattcg gcgggctgga gtagttgcgg cgaactgttg ctttcaattg
     9121 ccccagtacg cggccagcgg cttcggcatc ttcacacaga acagaaagtc cgccgacgcg
     9181 ctcgccgtaa agggagaaaa ttttcgagaa cgaattgctc accagagcgg gtaatccagc
     9241 gctggcaatg gcgcgaatag cgtaggcatc ctcttccata ccggcactaa atccttgata
     9301 ggcaatatcg aggaatggga taagctcgcg ggctttgaga atttcaatca ccgcatccca
     9361 ctgatcatta gtgagatcgg cacccgttgg gttgtggcaa catggatgca gcaacacaat
     9421 actgcgggca ggtaatgttt tcagcgtcgc caacaggtca ttaaagcgca cgctgttagt
     9481 cgcttcgtca taccaggggt aagtactcac ttcgaatcca gccccggcga atattgctac
     9541 gtggttttcc caggtaggat cgctgaccca gacgcctgat tccgggaagt agcgtttcag
     9601 gaaatccgcg cccactttca atgcccctga gccgccaagg gtttgaatgg ttgctacgcg
     9661 ctgttgttgc agtaccggat ggtccgcacc aaacagcagc ggcgcaatgg catggcgata
     9721 gctgttaagc ccttccatcg gtaaataaag cgaagcgcca tggggctgcg cattcaggcg
     9781 cgcttccgcc tccgccacgg ctttcagttg tggaataatt ccgtcttcgt tgtagtacag
     9841 accgatactt aaattcactt tgtcgctgcg agggtcttct ttaaaacgct ccataagcgt
     9901 aagaatcggg tcgccagcgt aggcgtcaac tttttgaaac acgcgatggt tctccaggtt
     9961 tacgggcagg tggttaaaac acaataaacc ggaagaaggc gaagatcgag tggatgttca
    10021 ggagcgaacg gcaattagca acagagtgag actcatgaca aacgaacatc cgccagaggc
    10081 acgaccttta taagagcatg aaagaacatc aacttattga atttttagga ttttattggc
    10141 cggataaggc attcacgccg catccggcac agacaatcaa atattacaga acgattaatc
    10201 cacgtatttc atcgcgaccc ttgaagtcag gcgcgtaata agttcgtaag cgctcacttt
    10261 tgtcatttca gcgatacgtt ctacgggcaa accttcgccc cataaaatga ccggatcccc
    10321 ggctttgtcc tgcgcctgtg gacctaagtc tacgcagatc atatccatcg ccacgcgtcc
    10381 gacaatcggt acttcgcgac cgttcaccag cactggcgta ccggatggag cggcgcgcgg
    10441 ataaccatcg ccatagccca tcgcgactac gccaagacgg gtatcacgtt cgcttaccca
    10501 ggttccacca taaccaacag gctctccggc tttatgctca cgcacggcaa tcaggctgga
    10561 ggttagtgac atcactggct gacagccaaa atcggcaccg atggagcgat cttccagcgg
    10621 cgagacgcca taaagaatga tgcccgggcg cacccagtca aaatgcgatt gtggccacag
    10681 cagaatgcca cccgacgcgg caatggaacg ttgaccaggt ttgccttcgc aaaaggtatt
    10741 aaagatagcg agttgtttct cggttgcgcc acattttggt tcatccgcgc gcgcaaaatg
    10801 gctgacgata ttcaccggct gacgaacgtt tttgcactgt gtcaggcgat gataaaacgc
    10861 ctcagcctgt tccggcctta cgcccagacg gtgcataccg gtatcgagtt tcatccagac
    10921 ggtaaccggc tcgtccaggc tagcctcttc cagcgcagcc agctgttctt cgttatgcac
    10981 ggcggtatga aaatgttgcg cagaaatcgt cggcagatct ctggcatcaa aaaagccttc
    11041 gagtaacagt acaggtttgg tgattccccc cgcacgcagt cgcagagctt cttcgagacg
    11101 ggctacgcca aaggcgtcag catcggggag cgttcgcgcg gtctcaagaa gaccgtgacc
    11161 ataagcgttc gctttcacca ccgcaaccat tttactggca ggggccagtt cacgaagacg
    11221 ttgcaggttg tgtcgcagag cgcggcggtt aatcacaaca gttgccgctt gcatttgtgt
    11281 tccttgataa gtgtttgctt taattaccta attcataaaa taattattat tcgtcgtcgt
    11341 actgcggccc cgcatagttg tcgaagcgcg accattgacc gttaaaggtc aggcgtaccg
    11401 tcccgattgg gccgttacgt tgtttaccga taataatttc cgcgatgcct tttaaatcac
    11461 tgttttcgtg atacacctca tcacgataga taaacataat caagtccgca tcctgctcga
    11521 tagagccaga ttcacgcagg tcggagttga ccgggcgttt gtcggcacgt tgttccagag
    11581 aacggttcaa ctgggacagc gccaccaccg gcacgttcag ttctttcgcc agtgctttca
    11641 gcgagcgaga gatttctgca atttccagcg tacggttatc ggaaagcgcc ggtacgcgca
    11701 tcagttgcag gtagtcgatc atgataagcc cgatgccgcc gtgttcacgg gcaatacggc
    11761 gtgcgcggga acgcacttcc gttggcgtca ggccggagga gtcatcgata tagatattgc
    11821 gtttttcgag caaaataccc atggtgccgg aaatgcgcgc ccagtcttca tcatcgagct
    11881 gcccggtacg gattttagtc tggtcaacgc gcgacagcga cgccagagaa cgcatcataa
    11941 tctgttctga aggcatctcc agcgagaaga taagtactgg tttatcctgc aacatcgccg
    12001 cgttttcgac gaggttcatc gcaaatgttg ttttacccat cgacggacgc gcggcgacga
    12061 tgatcaaatc cgacggctgc aagccagcgg tttttttgtt gagatcgtca taaccggtgt
    12121 ttactccggt aacgccatcg tgtggctgct gaaacaactg ctcaatacgc gccacggttg
    12181 cgtcgagcac atcggcgatg ttcttcggcc cttcgtcttt gtttgcacga ctttcggcaa
    12241 ttttaaagac gcgggattca gcaaggtcca gcagatcttc gctggtacgc ccctgcggat
    12301 caaaaccggc ttcagcaatc tcattcgcaa ccgagatcat ctcacggaca acggcacgtt
    12361 cacgcacgat gtcagcataa gcactgatgt tcgccgcact tggcgtattt tttgacagct
    12421 ctgccagata agcaaaacca ccgacgctat cgagttgccc ctggcgttcc agcgattccg
    12481 caagggtaat cagatcgata ggactaccgc tttcctgcaa acgcgccatt tcagtaaaga
    12541 tatggcggtg tgggcgggtg taaaaatcgt ctgccaccac acgctcggct acatcgtccc
    12601 agcgttcgtt atccagcatt aaaccgccca acaccgactg ctccgcttcg atcgagtgcg
    12661 gaggcacttt cagcccggca acttgtggat cgcgttcgcg gggttcagcc tgctgtttgt
    12721 tgaagggttt atttcctgcc atagtgaatg gagttaccga gataaagaat gggtcgaaac
    12781 tttaccatat gaagcagacc ctgacgatac gttctggagg acacatggca acacgaattg
    12841 aatttcacaa gcacggtggc ccggaagtac ttcaagccgt agagttcact cctgccgatc
    12901 cggcagagaa tgaaatccag gtcgaaaata aagccatcgg catcaatttt atcgacacgt
    12961 atatccgcag cggcctttac ccgccgccat cactacccag cggattaggt accgaagcgg
    13021 caggcatcgt gagtaaagtc ggcagtggtg taaagcatat taaggcaggc gatcgtgtag
    13081 tctatgcgca gtcggcgtta ggcgcttaca gctctgtgca taacattaat gcggataaag
    13141 cggcgattct gcctgcggca atttcttttg agcaagctgc ggcatccttc ctgaaaggct
    13201 taacggttta ttatctgctg cgcaaaacct atgaaattaa acccgatgaa cagttcctgt
    13261 tccacgcagc ggctggcggc gttggcttaa ttgcctgcca gtgggcaaaa gccctgggcg
    13321 cgaaacttat cggcaccgta ggaaccgcgc aaaaagcgca gagcgcgcta aaagcgggcg
    13381 cgtggcaggt tattaactat cgtgaagagg atctggtcga gcggttaaaa gagatcaccg
    13441 gcggtaagaa agtgcgcgtg gtgtacgatt ccgtgggcag agacacctgg gaacggtcgc
    13501 tggattgcct gcaacgccgc ggcttaatgg tcagttttgg caactcatca ggtgcggtta
    13561 ccggtgtgaa cttaggcatt ctcaatcaaa aaggctcgtt gtatgtgaca cgcccttccc
    13621 tgcaaggcta tatcaccacg cgggaggaat taaccgaggc cagtaatgaa ctgttctctt
    13681 tgattgccag cggtgtgatt aaggtcgatg tcgccgagca gcagaaatat ccgctgaagg
    13741 atgcgcagcg tgcgcatgag attctggaaa gccgggcgac gcaaggttcc agcctgttga
    13801 ttccataaaa gaaatagggc ttccacctgg gaagcccttt ctttttatag ttcggctgta
    13861 tgtagggtac agcacgatga atctgttaga ggcgcaatag tgacagattt gattatcaat
    13921 tcctattttg ttctaaggat aaaaccttag gttgtgatca tccgcacaat cccttagtaa
    13981 cgccagcggt cataacgctg atatttcggc acttttggtg ctttaatcgc cttaataacc
    14041 cacaccaccg caatcgccag tagtaaccac ggcagcaact taatcatcaa tgccagcata
    14101 ccgccgagga acataatggc cgtcgccaca accagcgcgg cgataatgcc cagcaacgaa
    14161 acgccggtga ccatcagcat gacaaaaaag ccaatcacaa aaagtagttc cagcatgatg
    14221 ctctcccaaa tatgaaatct cttgctggca ttacaagaat catgccaaaa ataatctatt
    14281 gatttaacag caaaacgccc cgcgacggtg cgcagggcgt ggtgaatttg actacttttt
    14341 ggtgaaaagt taacgcttat ccgccaccag tttgagcgcg tgttccagca cattaatgtc
    14401 tgcacccgct ttatgggcat tttcacttaa ataacgccgc cactgccgcg cgccaggaat
    14461 accctggaac aagcccaaca tatgccgggt aatatggccg agatacgtcc cctggctgag
    14521 ttcacgctca atgtacggat acatggcgcg cactaccgcc accggatcgg catcggtatc
    14581 cgaggaacca aagatctccc ggtctaccgc cgccagaata cccggattct gatacgcctc
    14641 gcgcccgacc atcacgccat ccatatgttg caggtgtgct ttggcctctt ccagcgactt
    14701 gataccaccg ttaatcgaca ttgtcagatg cggaaagtca cgcttcagtt gatacacacg
    14761 cggataatcg agcggcggga tttcacggtt ttctttcggg cttaaccccg aaagccaggc
    14821 tttacgtgcg tggatgataa acatctcaca ctcgcctttg ccggaaacgg tgttgatgaa
    14881 atcgcagaga aattcatagc tgtcctggtc atcgatgcca atacgcgttt tcaccgtcac
    14941 tggaatcgac accacatcgc gcatcgcttt cacgcagtcg gcaaccagct gcgcattacc
    15001 catcagacac gcaccaaaca tgccgttctg cacccggtca gacgggcagc cgacattcag
    15061 gttgatctca tcatatccac gcgcttctgc cagcttcgca cactgtgcca gcgccgccgg
    15121 atcgctaccg ccgagttgca acgctaccgg atgttcttct tcactgtacg ccaggtaatc
    15181 acctttaccg tgaataatcg cccctgtggt caccatttcg gtatacagca acgtattgcg
    15241 ggaaagcaga cgcaagaaat agcggcaatg tctgtccgtc cagtcgagca ttggtgcaac
    15301 gctaaaccga ccactccagt gaacgtcagt tttttcaggc attgcgctgg tttggttgat
    15361 tttttgcatt tcagaattac cgtgcatttt caaatgtaga gattatttta tcgatatatc
    15421 atggggttat gttattcagc atcactattc aggaggctca atagcggggt actataccat
    15481 aacaacagga agcgcctgtc tcattgcaaa agaaaattga gatcctctca aggcatgaag
    15541 ctctcacgaa gtgatggaaa taatcttatt agccgttaac tttgttaagg ccaatgataa
    15601 acaatccagg ttcgacgata aataaaaaat cacacattaa actctggtga tatatctccc
    15661 tgctaatagc attgatagag aaaaaagaac ccaataaata ttgggtcctt ttatataatg
    15721 ccttccatac tatcgaagaa cttcacatat tatttctccg atttaacccc gaacaaatca
    15781 taaattaatt tagaagtgtc tgtaagtatt ttaatctctt cctttgaggt tgggtcaaac
    15841 gacttcgcga agctaattaa ttgtggtgca gcatctcgca ttttgcttaa aatatcagga
    15901 tcgagcgttc cttcattcac cagatgtgac atcttatcca gatagccatc aaaattcatt
    15961 tctcccccat cagccagacg cttcatctct cccaggtact tcttcatatc acgttgagat
    16021 aatttttcaa actgagcttt caggtagctc tcattatttt cattaatata cactgtttct
    16081 gataaatctg cgtaggcact agaatatgaa agtgaaatta acagtgaagc aaataatttt
    16141 aagctgttgt catcatgttc ctggcaggca ttaacaaacg ttaaaaaacc agaacctatt
    16201 gcttgaaaat gtatattcgc taaaggttca ttatccttag attcctcata aaatagctga
    16261 accgtggagg gaaggccatt cttagtttct acatcaaaag tacattgttc aatgggagtc
    16321 gcatctttat tatccccagg attacatata ctattaatcg cgaaatgaaa aatagcccgg
    16381 tcaacaactg atgcaataaa gtcacgatcg tttaaatcct catcagtgca atcgtcaata
    16441 tacttattga cccacatttt gagatcattt atttgctcaa ttaaaaacac atttttttac
    16501 gtgcatgcca taaattatgc gtgattcgtt gaataaacaa atctattttt gcaataatta
    16561 tatcccgatg acgcagcgct gcataatcaa tagatttacc atatatatta tttaatttac
    16621 ctttgattga acctctatta tctaatttat ctttgattgc atcttcatta tcgaattgat
    16681 ttgaattgag tgttttatta aagtatccag ttgcaccggg tattatcttc aacatgattc
    16741 atctaccttt tttgtcgtac cgtgtcctcc ttataattct tttgccatct ggtcactatc
    16801 aaaaatcgct cataatttct gaactacagg tatgcgttta aacccagtta aataaatagc
    16861 gagttaactt gaagcaattt atttaaaaat gaattattaa ttattatcgt gttgataatg
    16921 gtacgacaag gcaacataac accttaccta caaaacagaa aatcaacatt acggcaacaa
    16981 taagggttct cggtgttttt cctggaacat ggtaaagtaa ggacattctt aacccccact
    17041 ttgaggtgcc cgatggaaaa gaccacaacg caggagttat tagcgcaggc tgaaaaaatc
    17101 tgcgcgcagc gtaatgtgcg cctgacccca cagcgcctgg aagtgttgcg cctgatgagt
    17161 ctgcaggatg gcgctatcag cgcttatgat ctgcttgatt tactgcgcga agctgaaccg
    17221 caagccaagc cgccaacggt ttatcgcgcg ctggattttc tgcttgagca aggttttgtg
    17281 cataaggtgg aatccaccaa cagttatgtg ctctgtcatc tgttcgacca gcccacgcat
    17341 acgtcagcca tgtttatatg cgatcgctgc ggcgcggtga aagaagagtg tgcagaaggc
    17401 gtggaagaca ttatgcatac gctggcggca aaaatggggt ttgccctgcg gcataatgtg
    17461 attgaagcac atgggctctg tgcggcatgc gtggaagtgg aagcgtgtcg tcatcctgaa
    17521 cagtgccagc atgatcactc tgtgcaggtg aaaaagaaac cgcgttaaga gggcgtacat
    17581 ccttgtacac gtcgggcagg agggattaat taccagcgat attcattgcg ggtttcccaa
    17641 tccacgacct ctttttctgc ctgatctttc tgataaccat aacgttcctg gattttaccg
    17701 accagttgat cacgtttacc ttcaatgatc gtcatatcat catcggtcag tttgccccat
    17761 tgctctttca ctttaccttt aaactgtttc cagttaccgc cggcttcatc tttattcata
    17821 atcaagacct catcgttagg ttgtgaatga gagtacgttc acttttcttc tgaacgtgag
    17881 attaagtgta gtcagcaatt tggcttagga taatttattc agaattttta accgtcacgt
    17941 tgcggcaaac caggtaccgt tacgccagtg acgccgccag atagccgcca gagaaagccc
    18001 gcgcaacgcc agaaagacgg ttagtgccag ccacaaacca tgattaccca gccacggcag
    18061 cgtaaggagc gtcagcgcaa aacctgcggc ggccaccgcc atactgttac gcatttcggc
    18121 ggcacgcgtt gcgcctataa acatgccgtc cagcagataa caccagacgc caaccaacgg
    18181 caaaatcacc tgccagataa gatagcggtc agccagctgc tgaatctggg ttaacgacgt
    18241 cagcaacgca atgatgtgtt cccctgccag caaataaacc accgaaaata gtaacgctac
    18301 gatccccgac tggcggcacg ctgcccgcca gacatccagc aactggctac cgtcgcgcgc
    18361 accgtatgcc tgaccggaat gtgcttcaac cgcgtaggca aaaccatcca gcgcataggc
    18421 ggtaaaggtg agtagcgtca tcagaaccgc gtttacagcg ataatgtcac tccccagtcg
    18481 cgcgccaagt acggtgatcg cgccgaaaca gagttgcaac aacagcgaac gcagcatgat
    18541 atcgcggtta agcgccagca agcgacggaa gtttcctcgc caggcagttt tcgacatttc
    18601 gccggaaatt ccgcgtagtt tgaggatttt acgcaccatt agcagaccaa tcagcaatgt
    18661 tgcatattcc gcaataaccg tcgccagagc cgcgccctgc acgttcatat gcagccccat
    18721 cactagccag acatccagca caatgttgag gatattgccg accactaaca aaattactgg
    18781 cgcacgggca tattgcacgc cgagtaacca accgagtaat accagattcg ccagcgacgc
    18841 cggtgcgctt aaccagcgga tttcaagaaa gcgccgcgcc tgttccagga ctgcttcact
    18901 accgccaaca atatgcagag ccagatcgat aatcggtgta cgcagcagcg caattaacgc
    18961 cccagccccc aacgccaaca gcaacggttg caccagcgca cgggctaatg cctgaggatt
    19021 tttggcacca taagcctgcg cagtcagccc ggtggtgctc atgcgtaaaa acagcaacag
    19081 cataaagaga aagctggtcg ccgttgcacc aaccgccacg ccgcccaaat aaaccgggct
    19141 atcaagatga ccaattaccg ccgtatcgac cagtcccagc aacggaacgg tgatattgga
    19201 gaaaatcatg ggtaaggcga gatgccagag tgctttatca gatgaagtga ggaatgccat
    19261 gcagacaagc ctgatgaaga gagatgaaaa acaaaccgcg acgcctggcg gcatcgcggt
    19321 ctcagagata tgttacagcc agtcgccgtt gcgaataacc ccaaccgcca gcccttcaat
    19381 ggtgaagctc tgctgacgaa ggtcaacgac aattggttta aactcgctat tttctggcaa
    19441 cagttcgact ttattaccct gttttttcag gcgcttaacg gtaacttcgt catcaatacg
    19501 tgcgacaacg acctgaccgt tacgtacatc ctgagtttta tgcactgcca gcaagtcacc
    19561 atccataatg ccgatatctt tcatcgacat cccgctgacg cgcagcagga aatcagcatt
    19621 cggcttgaac aaggaaggat cgacctgata atgaccttca atatgctgct gcgccagaag
    19681 cggttcaccg gcagccacac gacctaccag cggcaaccct tcttcctctt cctgcaacag
    19741 acgaatcccg cgtgatgcgc cggaaacaat ttcaataacg cctttgcgtg ccagcgcctt
    19801 cagatgttct tcagccgcgt ttggggaacg gaaccccaaa cgctgcgcga tttccgcacg
    19861 cgtcggcggc atacctgtct ggctgatgtg atcacggatg agatcaaaca cctcttgttg
    19921 cctggccgtt aacgctttca ttccgccccc tgggtgtata tacagttatg ctgtgagtat
    19981 atacagcaaa aggcgatttt ggaaccataa actgcacaat aaaccagaga tttatcgaaa
    20041 tctggaaggg ttatccaaaa tgcgaccata acaggatgca ccaggttatc acggcgacga
    20101 taatcgcaat cagcaccgcg gcggatccca tatctttagc gcgtccggaa agctcatggt
    20161 attcagagcc aattcggtca accactgctt cgatggcgct attgagaatt tccacaatca
    20221 tcaccagcat cacggagctg ataagcagca cgcgggtaat tgcgtccaca tccagccagc
    20281 aggcgatgac caccgccaac aataccgcta cgccttcctg acggaatgcc gcttcgttga
    20341 tccatgcagc gcgtaaacct ttccaggaat agccagcagc tttgataatt cgggtgaatc
    20401 cagtggtatt attggccatt gaaagaacct ttttacatta tgagcgtcaa tatcagtgta
    20461 ccgttaaatc cgatgattcg ggtttgcgta acgccacgcg aaacatgagc ggataccaca
    20521 gaatttccca tgactttctg ctatccttgc cgcgcatttg cattattaac cagaggcttt
    20581 acatcgttta tgtccggctg gccacgaatt tactacaaat tactgaattt accattaagc
    20641 atcctggtaa aaagcaagtc tattccggca gatcctgccc cggaactggg gctggatacc
    20701 tctcgtccaa ttatgtacgt tttaccgtac aactcgaaag cagatttgct gacgttacgc
    20761 gcccagtgtc tggctcatga cttgcctgac ccgttagagc cgctagaaat cgacggcacg
    20821 ctactgccgc gctatgtgtt cattcacggc gggccgcgtg tattcaccta ttacacgccg
    20881 aaagaagagt ctattaagct gttccacgac tatctcgatt tgcaccgtag caacccaaat
    20941 ctggatgtgc agatggtgcc agtatcggtg atgtttggtc gcgcgccggg gcgtgaaaaa
    21001 ggcgaagtaa acccgccgct gcgtatgctt aacggcgtac agaaattttt cgccgtactg
    21061 tggctcggtc gcgacagttt tgtgcgtttc tcgccgtcag tttcgctgcg ccgtatggcg
    21121 gatgaacacg gcacggataa aactatcgca cagaaactgg cgcgcgtggc gcgtatgcac
    21181 tttgcccgtc aacgtctggc tgccgtaggc ccacgtctgc ctgctcgtca ggatctgttt
    21241 aataagctgc tcgcctcccg cgccattgcc aaagcggtag aagatgaagc gcgcagcaaa
    21301 aaaatctccc atgaaaaagc gcagcagaac gcgattgcgc tgatggaaga gattgcggcg
    21361 aatttctctt acgagatgat ccgcctgact gaccgcattc tgggcttcac ctggaaccga
    21421 ctttatcagg gcatcaacgt ccataacgcc gagcgcgttc gccagctggc ccacgacggt
    21481 catgagctgg tgtatgtgcc ttgccaccgc agtcacatgg actacctgct actttcttac
    21541 gtgctgtacc accaggggct ggtgccgccg catatcgccg ccgggatcaa cctgaacttc
    21601 tggccggccg ggccgatttt ccgccgtctg ggggcgttct ttattcgccg tacttttaaa
    21661 ggcaataaac tttattccac cgttttccgt gagtatctcg gcgaactgtt cagccgtggt
    21721 tattccgtcg agtacttcgt ggaaggcggt cgttcccgca cggggcgttt gctggatccg
    21781 aaaaccggta cgctgtcgat gaccattcag gcgatgctgc gtggcggcac gcgtccgatt
    21841 acgctgattc cgatctatat cggttatgag cacgtcatgg aagtgggtac ttacgccaaa
    21901 gaactgcgcg gcgcgacgaa agagaaagag agcctgccgc agatgctgcg cggtttaagc
    21961 aagctgcgta atctcggtca gggttacgtc aacttcggtg aaccaatgcc gttgatgacc
    22021 taccttaacc agcacgtacc agactggcgt gaatctatcg atcccatcga agcggtgcgt
    22081 ccggcctggt taacgccgac ggtcaataat attgctgccg atctgatggt acgcattaac
    22141 aacgcaggcg cggcaaacgc catgaacctg tgttgtactg cgcttctggc atcacgtcag
    22201 cgctcactca cccgcgagca gttaaccgag caactcaact gctacctgga tctgatgcgc
    22261 aacgtacctt actccacgga ctctaccgtt ccttcagcca gcgccagcga gcttatcgat
    22321 cacgcgctgc aaatgaacaa gtttgaagtc gagaaagaca ctatcggcga catcatcatt
    22381 ctgccgcgcg agcaagcggt gctgatgacc tactatcgca acaacattgc gcatatgttg
    22441 gtgctgcctt cgctgatggc ggcaattgtc acccagcatc gccacatctc ccgcgacgta
    22501 ttgatggagc acgtcaatgt gctttaccca atgctgaaag cggagctgtt cctgcgctgg
    22561 gatcgcgacg agttgccgga cgttattgat gcgctggcaa atgagatgca acgtcagggg
    22621 ctgattaccc tgcaagatga tgagttgcat atcaacccgg cgcattctcg ccccctacag
    22681 ctgctggccg caggcgcgcg cgaaacgctg caacgttatg ccatcacctt ctggttgttg
    22741 agtgccaacc cgtcgatcaa ccgcggtacg ctggagaaag agagccgcac cgttgcgcaa
    22801 cgtctctccg tgctgcacgg catcaacgcg ccggagttct tcgacaaggc ggtgttcagt
    22861 tctctggtgc tgacgctgcg tgatgaaggg tatatcagcg atagcggcga cgccgaaccg
    22921 gcagaaacga tgaaggttta tcagttgctg gcggagttga ttacatcaga cgtgcgtttg
    22981 acgattgaga gtgcgacgca gggcgaaggg taatcagaga gaattgccgg atgcggcgaa
    23041 aacgccttat ccggccttcc ataatctgca aattcaataa attgcgatgt gttgtgtagg
    23101 cctgagaagc gcagcgcatc aggcaaccca gaagaaagcc ggatgatcat ccggcttttt
    23161 tacatcatca gaaatgccag taactcattg ccagtcctaa aaacagtacc agaccaacat
    23221 agttattatt cataaatgct ttaaagcagg cttcacgctc gcggttggca atcagttttt
    23281 gttgataaac aaacagcgcg ccagccacca gaattgacca gtaatatccc cagcctaagc
    23341 catttaactc accgatgatc gccatcagtg ccagtacgcc aatctgcaaa ataccaataa
    23401 tcaatttatc gtattggccg aacaggattg cggtggattt aataccaatc ttcacatcat
    23461 cgtcacggtc aaccattgca tactgcgtgt cgtaagccac cgcccagaga atattggcga
    23521 ggaacattaa ccagcaactc aatggcaccg actcactcac agcggcaaaa gccattggaa
    23581 tcgaccagcc aaacgccgcg cccagcacca cttgcgggag atgggtatac cgcttcataa
    23641 acgggtacac ccacgccaac gccagagcgg caatcgacaa cagaatggtc atcgtattca
    23701 gcgtcagcac cagtaaaaat gaaatcaata ccagcactac aaacagcgcg cgcgcctctt
    23761 tctccgttac cgcgccgctg ggaagcggtc ggttcgccgt gcgcttaaca tgaccatcaa
    23821 acttgcggtc agcataatca ttcaccacac atccggcagc gcgcatcagc cagacacccg
    23881 cgacaaacac cgccaggatc cagagctggg gaacgcccgg tgtcgccacc cacaacgccc
    23941 ataatgttgg ccagagcagc agtaacgcgc caattggctt atccgtacgc attaagcgat
    24001 gaaacgccag cagcttattc tgcgtcagac tccactccat atttttttcc tcttagtaca
    24061 acggtgacgc cggtaaaaac agttctgtta gcaacagcgg tttaccgctt aatcgcaggc
    24121 gggaacgtcg cccccacagt ccggcatcac ggcctatctc aataaagtcc cgggttaatg
    24181 tcgatgatgt gaacagatag cgtcctagcg gcgttttacc caatttttgt aacgccagct
    24241 ccggcccgct taacgttgac acaggaacga cggtacgacc ggcaagccac ggttcaccat
    24301 cggcacataa caaaatttca cgtaaccagt aacgagactc tttcggcagc agcggcagtt
    24361 cttcggggat ttcattctgc tcgacaaacc cttcgcggat catcgtcacg ctcaccgttt
    24421 ttccctgctg ttcaaaacgt tttgtcatgg aatcttccag caacagccag tcgagcagtt
    24481 gcgaatccag ggcagggatc tctttaaaat agcgcagcgc acgcagttgc gttaacgcgg
    24541 ggtgtgacat gccgaactct ccgttacata acgtaaaggc attgtatcgc agaaaagtgt
    24601 ttacgggaga tgtaaagcaa attaagtgat ctaatgcgca acaaaaacgc aacaatcgca
    24661 tgaagtggta aaacgctgtt gaaccttacg ctttgccgat gcccgtgctg gcgagggcaa
    24721 ccgtaggggc atcaccgccc atgatgccag gaacggtgat gttttccttt acttaaatac
    24781 ctatattctt tatacgagta gctcgccaac tcgcagtaaa aattgggaaa agcgcatatt
    24841 taccatgaga tttggagcta aaaagtgttt cctgagtaag attaataggc aacatcttat
    24901 gtctggattt atagatacac gtggaattcc aattggtatc ttttgtagga ttcaacatat
    24961 c
//
LOCUS       DAJVQG010000051        24454 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJVQG010000051 DAJVQG010000000
VERSION     DAJVQG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24454
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.051"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..174)
                     /gene="dgt"
                     /locus_tag="OQM83_001840"
     CDS             complement(<1..174)
                     /gene="dgt"
                     /locus_tag="OQM83_001840"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="forms a homotetramer; requires magnesium for
                     activity; catalyzes the hydrolysis of dGTP to form
                     deoxyguanosine and triphosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HCS1520118.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLE"
     gene            258..956
                     /gene="mtnN"
                     /locus_tag="OQM83_001841"
     CDS             258..956
                     /gene="mtnN"
                     /locus_tag="OQM83_001841"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCS1520119.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            949..1749
                     /gene="btuF"
                     /locus_tag="OQM83_001842"
     CDS             949..1749
                     /gene="btuF"
                     /locus_tag="OQM83_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCS1520120.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            1787..2410
                     /locus_tag="OQM83_001843"
     CDS             1787..2410
                     /locus_tag="OQM83_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCS1520121.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(2457..2801)
                     /gene="erpA"
                     /locus_tag="OQM83_001844"
     CDS             complement(2457..2801)
                     /gene="erpA"
                     /locus_tag="OQM83_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCS1520122.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(2794..2859)
                     /locus_tag="OQM83_001845"
     CDS             complement(2794..2859)
                     /locus_tag="OQM83_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1520123.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            complement(2883..4304)
                     /gene="clcA"
                     /locus_tag="OQM83_001846"
     CDS             complement(2883..4304)
                     /gene="clcA"
                     /locus_tag="OQM83_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCS1520124.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            4529..5809
                     /gene="hemL"
                     /locus_tag="OQM83_001847"
     CDS             4529..5809
                     /gene="hemL"
                     /locus_tag="OQM83_001847"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCS1520125.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            complement(5967..7949)
                     /gene="fhuB"
                     /locus_tag="OQM83_001848"
     CDS             complement(5967..7949)
                     /gene="fhuB"
                     /locus_tag="OQM83_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCS1520126.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(7946..8836)
                     /gene="fhuD"
                     /locus_tag="OQM83_001849"
     CDS             complement(7946..8836)
                     /gene="fhuD"
                     /locus_tag="OQM83_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCS1520127.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            complement(8836..9633)
                     /gene="fhuC"
                     /locus_tag="OQM83_001850"
     CDS             complement(8836..9633)
                     /gene="fhuC"
                     /locus_tag="OQM83_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCS1520128.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(9684..11873)
                     /gene="fhuA"
                     /locus_tag="OQM83_001851"
     CDS             complement(9684..11873)
                     /gene="fhuA"
                     /locus_tag="OQM83_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCS1520129.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSTAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            complement(12093..14627)
                     /gene="mrcB"
                     /locus_tag="OQM83_001852"
     CDS             complement(12093..14627)
                     /gene="mrcB"
                     /locus_tag="OQM83_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCS1520130.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(14823..17252)
                     /gene="hrpB"
                     /locus_tag="OQM83_001853"
     CDS             complement(14823..17252)
                     /gene="hrpB"
                     /locus_tag="OQM83_001853"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCS1520131.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            17326..17856
                     /gene="thpR"
                     /locus_tag="OQM83_001854"
     CDS             17326..17856
                     /gene="thpR"
                     /locus_tag="OQM83_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCS1520132.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            17871..18575
                     /gene="sfsA"
                     /locus_tag="OQM83_001855"
     CDS             17871..18575
                     /gene="sfsA"
                     /locus_tag="OQM83_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCS1520133.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            18753..19208
                     /gene="dksA"
                     /locus_tag="OQM83_001856"
     CDS             18753..19208
                     /gene="dksA"
                     /locus_tag="OQM83_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCS1520134.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            19245..20171
                     /gene="gluQRS"
                     /locus_tag="OQM83_001857"
     CDS             19245..20171
                     /gene="gluQRS"
                     /locus_tag="OQM83_001857"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCS1520135.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            20210..21628
                     /gene="pcnB"
                     /locus_tag="OQM83_001858"
     CDS             20210..21628
                     /gene="pcnB"
                     /locus_tag="OQM83_001858"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCS1520136.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            21625..22104
                     /gene="folK"
                     /locus_tag="OQM83_001859"
     CDS             21625..22104
                     /gene="folK"
                     /locus_tag="OQM83_001859"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCS1520137.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            22474..23058
                     /locus_tag="OQM83_001860"
     CDS             22474..23058
                     /locus_tag="OQM83_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1520138.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            23163..23903
                     /locus_tag="OQM83_001861"
     CDS             23163..23903
                     /locus_tag="OQM83_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCS1520139.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            23938..24454
                     /locus_tag="OQM83_001862"
                     /pseudo
     CDS             23938..24454
                     /locus_tag="OQM83_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         6003 a         6654 c         6107 g         5690 t
ORIGIN      
        1 ctccagtgga aaaacctggg tcttttgttg cagacgacga attgccggag agttgatgat
       61 acgcccgcga tcgctctcga agatccgcag gatctcatgt tcggttttaa cgccctgcgg
      121 tgaacggtaa cgacgatgcc agtttatttt ttttcggaaa tcaatctgtg ccatgtcctc
      181 tcccgcgtga gaaatacgct tccccgtaag cgcatggtaa actatgcttt caaatcgggc
      241 ttatcgcgag taaatctatg aaaatcggca tcattggtgc aatggaagaa gaagttacgc
      301 tgctgcgtga caaaatcgaa aaccgtcaaa ctatcagtct cggcggttgc gaaatctata
      361 ccggccaact gaatggaacc gaggttgcgc ttctgaaatc gggcatcggt aaagtcgctg
      421 cggcgctggg tgccactctg ctgttggaac actgcaagcc agatgtgatt attaacaccg
      481 gttctgccgg tggcctggca ccaacgctga aagtgggcga tatcgttgtc tcggacgaag
      541 cgcgttatca cgacgcggat gtcacggcat ttggttatga atacggtcag ttaccaggct
      601 gtccggcagg ctttaaagct gacgataaac tgatcgctgc cgctgaggcc tgcattgccg
      661 aactgaatct taacgctgta cgtggcctga ttgttagcgg cgacgctttc atcaacggtt
      721 ctgttggtct ggcgaaaatc cgccacaact tcccacaggc cattgctgta gagatggaag
      781 cgacggcaat cgcccatgtc tgccacaatt tcaacgtccc gtttgttgtc gtacgcgcca
      841 tctccgacgt ggccgatcaa cagtctcatc ttagcttcga tgagttcctg gctgttgccg
      901 ctaaacagtc cagcctgatg gttgagtcac tggtgcagaa actggcacat ggctaagtca
      961 ctgttcaggg cgctggtcgc cctgtctttt cttgcgccac tgtggctcaa cgccgcgccg
     1021 cgcgtcatca cgctttctcc cgccaacact gaacttgcct ttgccgccgg gatcacgccg
     1081 gttggggtca gcagctattc cgactatcct ccacaagcgc aaaagattga gcaggtttcc
     1141 acctggcagg ggatgaatct ggaacgcatt gtcgcgctga aacccgatct ggtgattgcc
     1201 tggcgtggag gtaatgccga gcggcaggtt gaccagctgg cttcgctggg aataaaagtg
     1261 atgtgggtcg atgcgacaag cattgaacaa attgccaatg cgttacgtca attggccccc
     1321 tggagtccgc aaccggataa ggccgaacaa gccgcgcaat ccctgctgga tcagtacgcg
     1381 caattgaaag cgcaatatgc tgataaacct aaaaaacgtg tttttctgca attcggcatt
     1441 aatccgccat ttaccagtgg aaaagagtcg attcaaaacc aggtactcga agtttgtggc
     1501 ggagaaaaca tctttaaaga cagccgggtt ccctggccgc aagttagccg cgaacaggtg
     1561 ttagcacgct cgccacaggc gattgtcatt acaggcggac cggaccaaat tcctaaaatc
     1621 aaacaatact ggggtgaaca gctcaaaatt cccgttattc ctctcacgag tgactggttt
     1681 gaacgtgcaa gcccacgtat tatcctcgct gcacaacagc tctgtaatgc gctttcacag
     1741 gtagattagc gcctgacctt tttaggtgct cagtggggat tgaacaatgc tcgtctattg
     1801 gctggatata gtcggcacag cggtatttgc catctccggc gttttgttag ccggaaaatt
     1861 gcgtatggac ccttttggtg ttctggtact gggcgtggtt accgctgtag gcggcgggac
     1921 aattcgcgac atggcgctgg atcacggccc ggtattttgg gtgaaagatc ccaccgatct
     1981 ggtcgttgca atggtaacca gcatgctgac catcgtgctg gtgcgccagc caagacgctt
     2041 accaaaatgg atgttgccgg tgctggacgc cgttggtctg gcggtgtttg tcggcattgg
     2101 cgtgaataaa gcctttaatg cggaagtcgg tccgttaatc gcggtttgta tggtcgtcat
     2161 tactggcgtt ggcggcggga tcattcgtga tgtactggcc cgcgagatcc ccatgatttt
     2221 acgtacagaa atctacgcaa ctgcctgtat tatcggcggt attgtccacg ctacggctta
     2281 ttacacattt tccgtaccac tggaaacagc cagtatgatg ggcatggtcg tgacgctatt
     2341 gattcggctg gcggctattc gttggcatct taagctaccg acgtttgcgc tggatgagaa
     2401 tgggcgttga agagagaaga tgcgccggat agtttatccg gcacatgaac aacagattag
     2461 atgctaaagg aagaaccgca accgcaggtg cttttcgcgt tcgggttggt cacgatgaaa
     2521 cgagaacctt ccagtccttc ggtatagtca acggaaccgc cgaccagata ttgcaggctc
     2581 atcggatcaa ccaccaagcc aacgccctgt ttttcgatgg tcatatcgcc ttcgttcacc
     2641 tgatcatcaa aggtgaaacc atactggaag ccgctgcaac cgccaccggt gatatacacg
     2701 cgtaatttca gattcgggtt atcttcgtca gcgatcaggc ttttaacttt gttggctgct
     2761 gcgtcggtaa actccagcgg cagtgctacg tcatcactca tattttgctc caaacgacat
     2821 cggcaattgg gcaaattcta acccaataat aatcgccatt atctaatacc ctggtatttc
     2881 gttcaagtat tctcgctggc tgatgcggcc ttgcttcgcg ccagttgctc agcttcctgt
     2941 tttgccagcg tgcgcgcaag aatcgccgag tatagcggtt tcccgccggt aaattgcgct
     3001 aatagtgttg cgccaagacc ggtaataatc attggcaaaa tgagctggta gttatcggtc
     3061 atctccagaa ccagaatgat ccccgttaac ggcgcgcgaa tagatgccgc cagtaatgcc
     3121 cccattccgg caatagcaaa cgtccccgcc tcaaggtgat attgcggaaa cagctcaacg
     3181 gcaaccattc cgaaagcggt tcccagcaca gtacccagcg ccagcatcgg ggcaaaaata
     3241 ccgcccggcg cgccggaaga gaagcagagt aaggtggtaa tgacccgagc gacgaagata
     3301 aacaccagca ttcccatgct gaaattcccc gcggtagcga taggaatcag gttaaaaccg
     3361 ccgcccgacg ttgctggtgc cacaaacccc agcaatccac acagaccgcc aatcgcaccg
     3421 cccatcagta cccatttggt aatattgccg ccgtgcacac ggtgcagcaa atcctgcatc
     3481 cccagcaccc atttattaaa aatagggccg aaaatgccaa aaataatacc gaggatcaga
     3541 taaagccaca gcgtattaag cggcgcatca gaaagtttac cgacgtcaat caacgcaact
     3601 tcatgattaa aaatccggta cataatggtc gacataatga cgccaataaa taccgctttt
     3661 atcgaaatta acgtatagcg aaactgcgga cgcatctctt cgataataaa caaaataccc
     3721 gccagcggcg cgttaaaggc cgcagccagc cccgcagcag caccggttgc cagcagcgta
     3781 tggcgagctt cgtcaccttt caggcggaaa atatcaagca ccatacggcc aatgttaccg
     3841 ccgatctgca cggttggccc ttcgcgcccc aacaccatgc ctccgccgag tgtccccagc
     3901 ccgccaaaga acttcaccgg caatacacgc caccagcgaa cgggacgttg atcttccagc
     3961 gccccttcaa tttccgggat ccccgaacca cctgcttccg gcgcgtattt gcgcaccaaa
     4021 aagtagccaa acatcgccag caccgccgaa cagagaaaag cgacggttaa cagaagcgga
     4081 taattatcag cagtatgtac cagcgccccc atacgttggt tctgcaacca ggcgacacct
     4141 ttgtcaaaag caaccgctgc cagcccaaca agcgtgccga cgactgccgc cataaacaaa
     4201 atggctaacg gggttttatc gcgctcaaga agttggcgaa tcagttgtct gcgtcgcagg
     4261 cgcgcggcct gcggtgtttc taaagaggga gtatcagttt tcatccaatg atcacttatt
     4321 ggtcatacaa ataagatgac ggcattttac tcgcacgttc catgaaaatc ctttgcaaag
     4381 cgtaatgttt caaatgcaca aaacttttat actctaagaa atcaggattg caccagtaca
     4441 agcagcctga tgtttgacga gtatttaact tgttatgaat aacatagaat agcagccatt
     4501 cactttttct acgaatcagg aaccctccat gagtaagtct gaaaatcttt acagcgcagc
     4561 gcgcgagctg atccctggcg gtgtgaactc ccctgttcgc gcctttactg gcgtgggcgg
     4621 cactccactg tttatcgaaa aagcggacgg cgcttatctg tacgatgttg atggcaaagc
     4681 ctatatcgat tatgtcggtt cctgggggcc gatggtgctg ggccataacc atccggcaat
     4741 ccgcaatgcc gtgattgaag ccgccgagcg tggtttaagc tttggtgcac caaccgaaat
     4801 ggaagtgaaa atggcgcaac tggtgaccga attggtcccg accatggata tggtgcgcat
     4861 ggtgaactcc ggcaccgagg cgacgatgag cgccatccgt ctggcccgtg gttttaccgg
     4921 tcgcgacaaa atcattaaat ttgaagggtg ttaccacggt cacgctgact gcctgctggt
     4981 gaaagccggt tctggcgcac tcacgttagg ccagccaaac tcgccgggcg ttccggcaga
     5041 tttcgccaaa cataccttaa cctgtactta taacgatctg gcttctgtac gcgccgcgtt
     5101 tgagcaatac ccgcaagaga ttgcctgtat tatcatcgag ccggtggcag gcaatatgaa
     5161 ctgtgttcca ccgctgccag acttcctgcc aggtctgcgt gcgctgtgcg acgaatttgg
     5221 cgcattgctg atcatcgatg aagtaatgac cggcttccgc gtggcactgg ctggcgcaca
     5281 ggattattac ggtgtggaac cggatctcac ctgcctgggc aaaatcatcg gcggtggaat
     5341 gccggtaggc gcattcggtg gtcgtcgtga tgtaatggat gcgctggccc cgacgggtcc
     5401 ggtctatcag gcgggtacgc tttccggtaa cccaattgcg atggcagccg gtttcgcctg
     5461 tctgaatgaa gtcgcgcaac cgggcgttca cgaaacgttg gatgagctga caacacgtct
     5521 ggcagaaggt ctgctggaag cggcagaaga agccggaatt ccgctggtcg ttaaccacgt
     5581 tggcggcatg ttcggtattt tctttaccga cgccgagtcc gtgacgtgct atcaggatgt
     5641 gatggcctgt gacgtggaac gctttaagcg tttcttccat atgatgctgg acgaaggtgt
     5701 ttacctggca ccgtcagcgt ttgaagcggg ctttatgtcc gtggcgcaca gcatggaaga
     5761 tatcaataac accatcgatg ctgcacgtcg ggtgtttgcg aagttgtgat ccagcatggt
     5821 ttgtcggatg cggcgtgaac gccttatccg acctacgatt cggggccgtt tgtaggcccg
     5881 ataagacgcg ccagcgtcgc atcgggcatc cgttgtcgcg taaacgcttt atccgtccag
     5941 gcgtacaggg ccgttatatg gaaaaattaa cggctctgct ttctcagcaa atagataaaa
     6001 tatggcgcgc cgataaaggt cgacagcagc cccgccggga tctggaatgg aaacagcacc
     6061 atccgcccac accagtcagc gaataccagc agtaaaccac ccaccagcgc cgaaattacg
     6121 atgtgtggca tcgtccgtcg aaagcccatc atccgcgcaa tatgcggtgc cattaaacca
     6181 acaaaactca acggtccaat agtcatcgtc gcggtcgccg tcaggcaagc cgctaacagc
     6241 agcagcgcaa ttcgcgtcgg cgtcagcgcc attcctacgg ctcgtgcggt atcaccaccc
     6301 agcggtaaaa tggtcagcca gcggcggtac agcggggtaa tcgccagcaa aatcaccatc
     6361 acaattccgg tacgccagac ctgcgcatcg gtcgcgttgt aggtcgaacc ggaaatccag
     6421 gtcagcactt gcgccattcg cgggtcaccg ctcgcctgca acatcatcaa aagcatggtg
     6481 aatgcggtac ttaacgccat ccccgccagt aacatacggt gtggggaaaa tccaccgcgg
     6541 ccggcggcga tcataatgat caacagcgtc accgccgccc cgatactgcc cgcgggtaac
     6601 agccagccaa aggcattacc cggcaccaga aacagcatta acaccacgcc aaacgccgcg
     6661 ccagagctaa tccccagcac ttccgggctt gccatcgggt ttccggtcag ccgctgaata
     6721 atacagcccg ccaccgccag catgacgccc gcaaacagcg ccgccataat tcgcggccag
     6781 cgccagggca tcaaatcctc gagcaacgcc ccgctcgccc acgtccagcc gtgcgcatca
     6841 cgaccaaacg acagcgccac caccacagcc atcaacagca gcacgccgcc cgcgagggca
     6901 aacgccagca catgttggcg ttcagccgcg acacgatcgt tgaccttcat atccggcgcg
     6961 ctaatgctgc gtaaacgcgg caacagccac agtagcagcg gcgcaccgat caacgcagtg
     7021 accgaaccgg tggacacttc catccacacg cgagtcagcc agaggatgat ttgatcggaa
     7081 agccagagga tcagcgcccc aatcagcgac gccagcatca accgtggcaa caaacgccgc
     7141 gcccccagca ttttcgccag cagcggcgcg aacaaaccga taaagccgat aatccccacc
     7201 gcattcacca gcagcgcact gatgactatc gccagcgaca gcgccgccag acgcgcgagc
     7261 gacaaggcca gcccgagatt gcgcgccacg ccatcatcaa gccccatcag ggttaacggg
     7321 cgaagtagca ataacgtcaa catcacgccg cccagcaact gcggccataa acgctcaacg
     7381 ccgccccagt cggtttgcgt cagcgttccg gtgctccaca ggaacatgct ttgcagctgg
     7441 tcgtgatgga agataaccag tagctgatta attgccccgc aataaaggct caccaccagc
     7501 cccgcgagaa taagcgtaac cggcgaaagc cgtttccccc acgcgacgcc aaagacgatt
     7561 aagccaacga cacaagcgcc caccagtgcg gcaaactggc tcgccatagc acctgggatc
     7621 gcccaaagtg tggtgacggt aattcccagt tgcgcgcctg tagcaacgcc aagcgtcgtc
     7681 ggctccgcca gcgggttacg cagcacttgc tgaaacagca cgcccaccag ccccaggccc
     7741 gcgcccacca gcagcgaaat cgccagacgc ggcaacaagc tgtagtgaaa aatcatctgc
     7801 tcgatgacat cgatgtctgg cgaccaggca gcctgcgccc actggctacg cggcagcgcc
     7861 tgcgagaagt tcatccaggt gagcgccgta gcgacaatca ctaacagcgc cagcaataac
     7921 gccggaaaaa gcgcaattcg tttactcacg ctttacctcc gatggcgtta tccagaacgc
     7981 gcacaaagtg cattgccgag agtgtcgcac cataaaacca gactgcaggt acgcgctgaa
     8041 agcgtccggc gcggacaaac ggcatggcct gccacagcgg ggttgccatt agcgcatcca
     8101 tgtctttgct gttgtcgtga tcaaaacaga gcacatcaac gtctttatac gctgccagac
     8161 gatcgatact gacggcggta ctgccccaga agttggtttc cccttgccag gcatttggga
     8221 tgccgtactc atcgagaatt tcctggaaca agctgtttgg accgaagacc agcatatggc
     8281 gcggatcgat aagcgtcgtc agcagtaacg ggcgtgcacc acgcttcaca aagcggggtt
     8341 tcatgctgcg gataaagtct tcgtattgcg ctaaatgcgt ttccgctgcg ctttgcaggt
     8401 taagtaaatc tgccatttcc gtcagcgatt tacgcgccat cgccaacggc tgtttgccgt
     8461 cactgaagtt aaatccgcga cccggcgcaa tacgtgccag catttctgat gaagggccat
     8521 atcctgccga ccagaccata aacgacggtt tcatttcggt cagcagttca aggttaggtt
     8581 ctgtacgcaa accgacgtca atcactgagt ccggcaatgg tggttcgctg acccacaggc
     8641 gatagttgat ggtatccgcc acgccgtaag gcacgatgcc gagcgccagc agtaattcca
     8701 ccggcaacca ctccagcgcc acaatacgat tggggtcaat agccgccgcg tgggcggtat
     8761 tcatctgcca taacaacgga gaaagcgcca tcgccgttaa cagtcgacgg cgcgaaataa
     8821 gaggtaagcc gctcatcaat aaacaaaact cacaggtgca gcacccgccg gatgcggcaa
     8881 aatacccatc gggatgccat aaatcatttc gagggtttcg ccgcgcataa tttccgcagg
     8941 cgttccctga gcaatcattt caccactgcg cagggcgacc agataatcac agtagcgtgc
     9001 cgccatattg atatcgtgca acacggcaat gaccgtcagg ccacgttcct gacttaaacg
     9061 gtgcaccagc gccagcacat caacctggtg ggcgatatcc agcgccgaag tcggttcgtc
     9121 gagcaacaga caacggctat cctgcgccac cagcatggcg atccacgccc gctgacgttc
     9181 gccgccagag agactatcga ccagccgatg cgccagcggt tttaagccaa ccagcgagat
     9241 agcttcctcg accttttcgc gatctgccgc accaaagcgc cccagcgcgc catgccacgg
     9301 gtaacgacca atcgccacca gttcacgcac ggtcatccct tctgccggag gaagctgctg
     9361 cggcaaataa gccactttgc gggcaaacgc tttgctgctc cagctttcca gcggctgggc
     9421 atcaagaaga atctcccctt ctgacggagg ctgatgacga ccaagcattt tgagcagagt
     9481 ggatttacca gaaccgttgt gaccaatcag accggtcact ttcccggcag gaaaggttaa
     9541 cgacagcgga tgcaaaagcg tgcgcccggg cacacgaaag gagatattac gcagtgcaaa
     9601 agtggtatcg gaatgattcg tgtattcctg cataacagcc aacttgtgaa atgggcacgg
     9661 aaatccgtgc cccaaaagag aaattagaaa cggaaggttg cggttgcaac gacctgacgt
     9721 tctgcgcccc agaagcagcc ataagtctgg aagcaactgg caacatactc acgatcgagc
     9781 aaattgttga cgttcacagc aaggctggat cccgccatac caaagcgtgc cagatcatat
     9841 tttacaacag catccattac agctgtgcta cccactttaa aagtgtttgc cggatcacca
     9901 tagctggaac caataaaacg accacctgtt cccaatgtta aaccagaaag cggcccttca
     9961 ttgaaggtat aatcccccca gagagatgcc atatgctccg gtacttgttc tggagtttta
    10021 cctttgagat tggtgtcttt ggtgtattcc gcatcggtgt aggtataaga agccgtcaag
    10081 ttaatattgg catttaacgc cgcttttgct tcaagttcca caccacgagc gcgaatctca
    10141 cctgctggga cttgcgctaa tgggtttgtt gggtctgctg tcaggttatt tgttttcgtc
    10201 aattgataga ctgcgcccgt aacaacgacc ggcatatcat tcggaacata ttttacgcca
    10261 gcttcatact gttcaccttt cgatggctta taggaaatgc gcgggttgct ccacagatcg
    10321 aaagcactcg gttcaaagga ctggctatag ctaaagtaag gtgaaatacc attatcgaat
    10381 aagtaattta caccaccgcg ccaggtgaac tgatgatcat tgcgctcaat ataaccttca
    10441 gtcggcgtat aagagttttc acgaacagtg gtcgcttgct tagaccaatc gtagcgtccc
    10501 cccagagtga atacccattt attccattcc gcctgatcct gaacataaat acctgtttgt
    10561 ttgctttcat tcatctggta tggctctgcg ctaccaaatg caaagtattc agggtgatat
    10621 ttgttataaa gatcgatgga tggtgcggat ccaaagctgg cattgatatc attacgcatc
    10681 cgcatgaagt ctacccctgt cagcaaagta tgctcgacgt cacctgtagc aaacttactt
    10741 tcaagttggg tatcaacgct aaagttttgt agacgctcat tatccaccac tgtcccgcgg
    10801 tttagggtat gaccatcacc agcaatccct gtgccataaa cacttttctg tgaggttttc
    10861 atttcactga aacgcagatt ctgacgcacg gtgaaggtgt cattaaaacc atgttcaaaa
    10921 ctatatccca ccattttctg gttacgggag tatgtattat tcgatgcacc ttcattgaag
    10981 tcagtcggta gacgcttacc attaggcaat ggctcaacgg tcccctcttt cggcaaccaa
    11041 ccgtaataac ccgtttctgg ttcattctgg aaataggaca ggaaagtaaa attggttttg
    11101 tcgtctggac gccatgagaa tgatggagca atggtataac gctgagattc agagtttttc
    11161 tgctgttcgt ttgttgagcg agcgaggccc gtcaaacggt aagagaactc accgttatca
    11221 tccagtgcat cactgaaatc gaatccggtc tgaaacagat tatccgtccc cattttaaac
    11281 tgaatttctt tcaggggttc agtggtcgga cgcttactca ccatagaaat aatcccacca
    11341 ggattacttt ttccatagag aacagacgtc ggcccacgca tcagttcaac cctctcgagc
    11401 atgtaaggat caatcacagc atcgttataa aagttgccct gaagttttag tccatcaagg
    11461 taattatttt gattcagacc aacagatgag aaaccacgaa tgataacaaa atcatatgta
    11521 ttagaagcac cacgactgct aaccgtaacg ccaggtgtat aacctaatgc ttcttttaca
    11581 gactgaaatt gacgcatctg catctcttca tttgtcacaa ccgaaataga ctgcggcgtt
    11641 ttttcaattg gtgtatccgt tttagtcgca gtagcagagt gcttcgccgc aatagttgca
    11701 gcaggccccc atgcgctttc ttgcggcgca ggtgcagcgg taacggtgat agtgtcttct
    11761 ttcggttcaa ccgctgcctg tgcataaaca gacatgccgc taaccgctgt ggctactaca
    11821 actgcgattt tacgcagtga gtgttttggc tgagcagttt tggaacgcgc cattggtata
    11881 tctctgatgt aaagtgaatg ataacgtaaa cgagaatgat tattataaag ataagataat
    11941 attcgaaacg ctggcgggtt cgcaagcggt acgcaatctg agcgttcctg aagggttaat
    12001 aaacaaccag atgaaaagaa agggttaata ctttaggtgg gatgttattt taccggatgg
    12061 caattcgcca tccggtattt cacgcttaga tgttaattac taccaaacat atccttgatc
    12121 caaccggcta caccgtcgct gtctttctgc tcttgctgag caggttgctg ttgcggctgt
    12181 tgctgcggct gagaagactg atcaaacgga ttgcctgacg gctgctgctg catctcgctc
    12241 tgctggcaca gcgattgcgg atcgctggtc cagaccggca agacacgcat gccaccgctg
    12301 caaacaaagt tgccgtcgta gtccacgccc atatctgcaa tatcttctgg cggaacaaga
    12361 ttcagcggcg ttggcgtctg gttagccaga taacgctgat aaatagacat tgccccgctg
    12421 gcaccataca gtttggtcgg ctggttgtta tcacggccta cccaggtgat ggtcaccgtg
    12481 ctgccgtcaa tgcccgcaaa ccaggtatct acgttattgt tggtagtccc tgttttccct
    12541 gccagatgca ggttcgggta tttcgcccca agttgacgac ccgtaccacg ttgtaccacc
    12601 tgctgcatgg tccatagtgt cagatacgcc gcctgcgccg gaacagcgcg ttctgcctgc
    12661 gggaagctct gatacagcac cttgccatct tccgcgatta ccgaacgcag tgcagaaagc
    12721 ggtgcacggt taccgccgct ggcgatggtc tggaatgcct gcgccacttc gattggcgtt
    12781 aagttcaacg cccccagcag catcgccgga accggatgca gctgatcttt cggcacgccc
    12841 agtttgatcc aggtctccgt aactgcaggc agccccagcg ccatccccag atttaccgtc
    12901 ggcacgttca tcgaacgggt caacgcatcc accagcatca ctctgccgct ttcgctataa
    12961 cgacggtcat cgttctgcgg tgaccagacc tggccattcg gctgacgcag cgcgattggc
    13021 gcatcggcaa tccacgtgtt caggcgatag attttcggct ggcttaaagc cgtcagataa
    13081 gtcgctggtt ttgcaaggga accaatcgaa cgacgcgcct gcatcgcacg gttgtagccc
    13141 gcaaactgcg gctcagaacc tcccaccatc gcacgaactt caccactaaa gcggtcgacg
    13201 accacaattg cagtttcaag atcgctcaac ttacgctgtt tcttcagtgc cggaatgcct
    13261 tccacggcgg ctttttctgc cgcgtcctgg gccaccgagt caaaggtagt gaagatcttc
    13321 acgccggaga gatcttttac cttatcgcct aatttcgcct gcagctcctg acgcaccagt
    13381 tgcataaagg ctggctgtgg agagattacc ccaccgcgcg gctgtacgcc cagaggacgc
    13441 gcgctgagca tttcatacag ttcaccatcg ataatctgct gttgttgtag caaacgcagc
    13501 accagattac gtcgctccag cgccagtttc gggttacgcc acgggttgta gatggacgcc
    13561 cctttcacca taccgactaa cagcgcctgc tggtcgaggc tgagctcttc taccgggcga
    13621 ccaaagtaat acaagctcgc aagcgggaag ccgcggattt cgttgtcgcc gctctgaccg
    13681 agatacacct cgttcatata cagctcaaga atacggtctt tgctgtaacg cgcgtccatg
    13741 atcagcgcca tgtaagcttc gttcgcttta cgccagtaag aacgctcgct ggagaggaac
    13801 aggtttttca ccagctgttg cgtcagcgta ctcgctccct gcaccgtgcg tccggcggtc
    13861 aggtttgcca gcaccgcacg tccgatggag tagagactga tcccatcatg ctcgtagaaa
    13921 tgacggtctt ctgtcgccag caaagtatcc accagcaagt ccgggaaacc actgcgcggc
    13981 acaaacagac gctgctcacc gtttggcgaa gagatcatgg tgatcaggcg cggatcaaga
    14041 cggaagaaac cgaactgacg gttgttctcc atattgacga tcgtcgccag atgatcgcca
    14101 tcaaaggtca gacgcgcacg cacctgccct tctttgctgt ccgggaaatc aaacggacgg
    14161 cggatcatct caatgctgtt ggcctgcacg gtaaattcgc caggacgggt cattttcgac
    14221 acctgacgat actgtgttgc ctccagcagc ttcaccattt cgttcttgct gatggtcata
    14281 tctggttcaa ggttgaccat tcggccataa actgccgcgg gcagttgcca gaccttgcca
    14341 tcaatacggc tacgaatttt ttgatcgaga taaacgccgt aaatggcgat cagcacggca
    14401 aaaacgatag ccagttttaa cagcagccat agccagccgc gtttgccacg aggcttacgc
    14461 cctttgcctt tgcccttacc tttgcgcggc atcggttctt catcctcata gtcatcataa
    14521 tcgtcgtaat catcgtcatc ttcgtaacga cgacggctta ccttttgttt gaccggacgc
    14581 gtcggtttcc ctttgcgtcc aattggctcg cggtcattcc cggccatgct ttttctccgc
    14641 aatattcagg cgcaaaagcc cgattttctg ttcttctgtt acaagacaga agaagatatc
    14701 tctcaaaatt tgtgctcgat cggtcccctc tcccttgagg gagagggtta gggtgagggt
    14761 gaacgtgcgc gctgatgccc tcaccccggc cctctcccac agggagaggg agaaaaccaa
    14821 ccttacgaat actttttcgt ccgtcgcgtc ggtgccgtgt ttgccgggtc gtccggccag
    14881 acgtgtttgg gataacgccc tttcatctct ttttgcacct cacggtacgc tcctttccag
    14941 aaggcgctca aatctcgtgt gatttgtagt ggcctttggg cgggtgaaag caactccagc
    15001 accagcggca cgcccccctg ggcgatagtc ggattagtgg cctcaccaaa catctcctgc
    15061 attctcaccg ccagtgcggg cgggttatct tcatgataac gaatggcgat ccggcttccc
    15121 gtcggcacag tgtaatgcgc aggcaattca ctatccagac gttgctgcat tccccaatca
    15181 agtaatccgc gtagtgcctg ataaatgtcc agtgatttca ggccgcgtag tgaatgtacg
    15241 cctgtcatat gtggcagcag ccacgtttcc agcgctgcca ataaactttc atcatcaacc
    15301 gctggccagt catattccgg caaccacttt gcggcgcata acaaacgcaa gcgtagctgt
    15361 tccgcttccg ccgtccagtt gagcacgctt aaacctttat cacggatgcc attaagcatc
    15421 gcctgatgca actcgtcttc tgacggtttc gccagcggct gcactttcac cgtcaactga
    15481 ccgatttgta gccgacgcca ggctttcagc gtaccttgcg cgtcatccca ttccacggtg
    15541 tcagattgct gcaccagctg cggacagcgt tgtactaatt catcaatatc gaccggtagc
    15601 gccagtaaaa tccgcgcatc cggcgaggcg ctgccctgca ataataacgg tgcgatcaac
    15661 cattcgtggc ggcttagcgc gtcgtcggca tcgagcatcg ctcccatgcc gtttgccagt
    15721 tgatagcgtc catcttgccc acggcgatga gcaatgcgat cggcaaacgc cccggcaagt
    15781 agcggcgcga taagcgaact gtctgcctca ccgccgcgta cgtttaagcg ttttaacagt
    15841 tgctgactac gttgctgcca ggctggttga ttgcgcgaaa acgccacgcc caggtcacta
    15901 ttgcccatca gtggcggctc ttcgagaatg gcggcaattt ttgccgcggt agcagcttcg
    15961 tcgtcgttct tcgcgctaac cagcattgcc gctaaacgcg gatcgttacc cagcgctgcc
    16021 attttttgcc cttgcgcact aagccgttca ccgtccagcg cccccagcat ttgtaacaga
    16081 cgtttcgcgg ccattagatt cactactggc ggttgatcca gccagctcat ctgcgccgga
    16141 tcgctgcatc cccattgcag taattccatc agcaaaccgg aaagatcgct ttgtaagatc
    16201 tccggttcac tttgcgcggc agcgcgttct gcttgttctt tggcgattaa atgcaggcag
    16261 atacccggct ccagacgccc ggcgcgcccg gcacgctgcg tcatggatgc ctggctaacg
    16321 cgttgagtaa tcagtcgcgt aagccccgtg cgcggctcaa aacgcgccac acgctcctgg
    16381 gcacaatcca ccaccagacg aataccttca atggttaaac tggtttcagc aatattggtc
    16441 gccagcacca ctttgcgcat cccttgcggt gccgggagga tcgcttttcg ctgatcgttc
    16501 agcgacaacg cgccatacag cgggcagagc aacacatcac tgccgatgcg cgaagccagt
    16561 tgttcctgca cacgctgaat ttctccgacg ccaggtaaaa ataacagtaa tgatccgctt
    16621 tcctgacgca gcatttcggc ggtggctacc gcaacggctt cgtcaaaacg ctgatgcgcg
    16681 ggcagcggta aataatggcg ttcaaccgga aacgagcgcc cttctgagat gacgacgggc
    16741 gcttctggca gcatttgctg caagcggtcg ttatccagcg tagccgacat aatcagcagt
    16801 ttaaggtcat cacgcagacc ttgttgcaca tcgagtaaca gcgccaacgc caaatccgcc
    16861 tgcaagctgc gctcatgaaa ttcatcaagg atcaccaatc caacaccgct caactccggg
    16921 tcacgctgga tcatccgcgt cagcacgcct tcggtaacta cttccagacg ggtatttggc
    16981 ccgacgcagt tttgcgcacg catccggtag ccaacggtat cgcctggctt ttcgttaagc
    17041 agctccgcca gccgttgcgc gacgttacgt gccgccagac gacgcggctc cagcaggata
    17101 attttcccgt taatgccggg atgcgccagc agttgcagcg gcagccaggt tgatttcccg
    17161 gccccggtcg gcgcacttaa taatacctgc ggcgcacaat cgagagcggt aagtaattca
    17221 ggtaagacgg cagcaacggg caacgacgac acaaaacgct ccagagggtt aacattcttc
    17281 gcgccacatt gtagcatcgc ggtaattcat aaccgagtgc ctcacatgtc tgaaccgcaa
    17341 cgtctgttct ttgctatcga cttacctgca gaaatccgcg aacagattat ccactggcgc
    17401 gccacacact tcccacctga ggcgggacgt ccggtcgccg ccgataattt gcatctgact
    17461 ctggcatttt taggcgaagt gagcgccgag aaagagaagg cgctttctct tttagccgga
    17521 cggattcgtc aacctggttt cacactcacg cttgatgacg ccggacaatg gctgcgttcg
    17581 cgtgtggtgt ggttagggat gcgtcagccg ccacgcggct taatccagct ggcgaatatg
    17641 ctccgttcac aggctgcccg cagcggttgt tttcaaagca atcgtccgtt tcatccacat
    17701 attaccttat tgcgcgacgc cagcgaggcg gtgacaatcc cgccgccagg ttttaactgg
    17761 tcttatgcgg tgacggagtt caccctttac gcctcctcgt ttgcccgtgg acgcacacgc
    17821 tacacgccgc taaaacgctg ggcgctaacg caataacaag gattgtcgca atggaattct
    17881 ctccccctct acagcgcgcg acgctaattc agcgttacaa acgtttttta gccgatgtga
    17941 tcacacccga tggtcgcgaa ttaacgctac actgcccgaa tacgggtgcg atgaccggtt
    18001 gtgcaacgcg tggcgatacc gtctggtatt cgacttcaga caacaccaaa cggaaatacc
    18061 cacacacctg ggaactaact caaagccaga gcggcgcatt tatttgcgtc aatacgcttt
    18121 gggctaacag gttgacgaaa gaggctatcc ttaatgaatc aatttcagaa ctgtcaggct
    18181 atagctcgct gaaaagcgaa gtaaaatacg gcgcagaacg cagccgtatt gactttatgt
    18241 tgcaggcgga ttcgcgtcca gactgctata ttgaagtgaa atcggttacg ttagcggaga
    18301 acgaacaggg atattttccc gatgcggtca ctgaacgagg tcagaaacac cttcgggagt
    18361 tgatgaacgt agcggctgaa ggccagcgtg cggttatctt tttcgccgtg ctgcattcag
    18421 ccattacacg gttttcaccc gcgcgccaca tcgatgagaa atacgcgcaa ctattgtcag
    18481 aagctcaaca gaggggggta gaaattctgg cttacaaagc ggaaatttct gctgaaggca
    18541 tggctcttaa aaaatcactg tcggttacat tgtagtaaag taagtaactg gttaatttac
    18601 attctggtcg cgtgcgcaaa tacgcttttc ctcacacagt tgtcaagtgt tacgtttaga
    18661 taattgctat ccggaaaagc atctgctatt tatagcggcc tcatttttcc cccgaacatg
    18721 gggatcgata gtgcgtgtta aggagaagca acatgcaaga agggcaaaac cgtaaaacat
    18781 cgtccctgag tattctcgcc atcgctgggg tggaaccata tcaggagaag ccgggcgaag
    18841 agtatatgaa tgaagcccag ctggcgcact tccgtcgtat tctggaagca tggcgtaatc
    18901 aactcaggga tgaagtcgat cgcaccgtta cacatatgca ggatgaagca gccaacttcc
    18961 cggacccggt agaccgtgca gcccaggaag aagagttcag cctcgaactg cgtaaccgcg
    19021 atcgcgagcg taagctgatc aaaaagatcg agaagacgct gaaaaaagtg gaagacgaag
    19081 atttcggcta ctgcgaatcc tgcggtgttg aaattggtat tcgccgtctg gaagcgcgcc
    19141 cgacagccga tctgtgcatc gactgcaaaa cgctggctga aattcgcgaa aaacagatgg
    19201 ctggctaatt acagccgttc catcacgttt accacaggcg ggaaatgctc ccgccttatt
    19261 ttttgttcaa agagatgaca gacacacagt atattggccg cttcgccccc tctccttccg
    19321 gcgagcttca ttttggctct ctgatcgccg cgctcggcag ctatttgcag gctcgcgccc
    19381 ggcaaggtcg ctggctggta cgcatagaag atatcgaccc gcctcgtgaa gttcccggtg
    19441 ccgcagaaac tatcctgcgc cagctggaac attacggtct gcactgggac ggcgatgttc
    19501 tctggcaatc gcaacgtcac gacgcctatc gtgaagcact cgcctggtta catgaacaag
    19561 gactaagtta ttactgcacc tgtacgcgtg cgcgtattca aagcattggc ggtatttacg
    19621 acggtcattg ccgggtgttg catcatggac cagacaatgc cgcagtgcgt atccgccagc
    19681 agcatccggt cacgcaattt actgaccagc tgcgcggcat tattcacgcc gacgaaaaac
    19741 tggcacggga agattttatc attcatcgcc gtgatgggtt gttcgcctac aacctggctg
    19801 ttgtggttga tgatcatttc cagggcgtta cagaaatagt gcgtggggct gatctgattg
    19861 aaccaacagt aaggcaaatc tcgctgtacc agctttttgg ctggaaagtg ccagattaca
    19921 ttcatctgcc gctggcgctt aatccacaag gcgctaaact ttccaagcag aatcatgcgc
    19981 ctgcgttgcc gaaaggcgat ccacgtccgg tactaatcgc ggcacttcaa tttctgggtc
    20041 agcaggcaga agcacactgg caggatttca gcgtcgaaca aatccttcag tcagcagtca
    20101 aaaactggac gctgaccgcc gtgcccgagt cggcaattgt aaattcaaca ttctcaaatg
    20161 cgtcatgctg agctatgatt agccgctatt tttttgtcct gaatgatgtt tgacactacc
    20221 gaggtgtact atttttaccc gagtcgctaa tttttgccgc aaggtgctaa gccgcgagga
    20281 aagcgaggct gaacaggcag tcgcccgtcc acaggtgacg gtgatcccgc gtgagcagca
    20341 tgctatttcc cgcaaagata tcagtgaaaa tgccctgaag gtaatgtaca ggctcaacaa
    20401 agcgggatac gaagcctggc tggttggcgg cggcgtgcgc gacctgttac ttggcaaaaa
    20461 gccgaaagat tttgacgtaa ccactaacgc cacgcctgag caggtgcgca aactgttccg
    20521 taactgccgc ctggtgggtc gccgtttccg tctggctcat gtaatgtttg gcccggagat
    20581 tatcgaagtt gcgaccttcc gtggacacca cgaaggtaac gtcagcgacc gcacgacctc
    20641 ccaacgcggg caaaacggca tgttgctgcg cgacaacatt ttcggctcca tcgaagaaga
    20701 cgcccagcgc cgcgatttca ctatcaacag cctctattac agcgtagcgg attttaccgt
    20761 ccgtgattac gttggcggta tgaaggatct gaaggacggc gttatccgtc tgattggtaa
    20821 cccggaaacg cgctaccgtg aagatccggt acggatgctg cgcgcggtac gttttgccgc
    20881 caaattgggt atgcgcatca gcccggaaac cgcagaaccg atccctcgcc tcgctaccct
    20941 gctgaacgat atcccaccgg cacgcctgtt tgaagaatcg cttaaactgc tacaagcggg
    21001 ctacggttac gaaacctata agctgttgtg tgaatatcat ctgttccagc cgctgttccc
    21061 gaccattacc cgctacttca cggaaaatgg cgacagcccg atggagcgga tcattgaaca
    21121 ggtgctgaag aataccgata cgcgtatcca taacgatatg cgcgtgaacc cggcgttctt
    21181 gtttgccgcc atgttctggt acccactgct ggagacggca cagaagatcg cccaggaaag
    21241 cggcctgacc tatcacgacg ctttcgcgct ggcgatgaac gacgtgctgg acgaagcctg
    21301 ccgttcactg gcaatcccga aacgcctgac gacgttaacc cgcgatatct ggcagttgca
    21361 gttgcgtatg tcccgacgtc agggtaaacg cgcatggaaa ctgctggagc atcctaagtt
    21421 ccgtgcggct tatgacctgt tggccttgcg agctgaagtt gaacgtaacg ctgaactgca
    21481 gcgtctggtg aaatggtggg gtgagttcca ggtttccgcg ccaccagatc aaaaagggat
    21541 gcttaacgag ttggatgaag agccgtcacc gcgtcgccgt actcgtcgtc cacgcaaacg
    21601 cgcaccgcgt cgtgagggta ccgcatgaca gtggcgtata ttgccatagg cagcaatctg
    21661 gcctctccgc tggagcaggt caatgctgcc ctgaaagcat taggcgatat ccctgaaagc
    21721 cacattcttg ccgtttcttc gttttaccgc actccaccgc tggggccgca agatcaaccc
    21781 gattacctca acgccgccgt ggcgcttgaa acttcgctcg ccccggaaga gttactcaac
    21841 catacacagc gcatagaact gcaacagggt cgtgttcgta aagctgaacg ctggggacca
    21901 cgcacgctgg atctcgatat catgctgttt ggtaatgaag tgataaatac cgaacgcctg
    21961 accgttccgc actacgatat gaagaatcgt ggatttatgc tgtggccgct gtttgagatt
    22021 gcaccggagc tggcgtttcc tgatggggag acgttgcgtg aagtcttaca tacaagagca
    22081 tttgacgaat taagcaaatg gtaatttatt tatattacga atataaatat tttctgaaaa
    22141 tcatacatcc tctctttcct taataaaaat tttagtttaa acatttttgc agaaacataa
    22201 tataaattcc cgcagataaa tatcaatccc atcgccatac cctaaccaat tgcacttgtg
    22261 aatttatttt tgcttaattt tgaattaaga aaatttaatt tgttcaaaag cccttaaagt
    22321 gcacaatagg cgtcctaatg aacaatggtt gtaaattttg gtaacaaata tcattgctca
    22381 ttaatatcta gcatctgctt ataacatttg tgtcatgaat agtcattctt caaaccagta
    22441 gtctgactaa tggaatttta ttcaaggatt tttatgtcta aaaaattagg ttttgccctg
    22501 tctggtctta tgctggcaat ggttgcaggt actgcttctg ctgatatgga tggtggtcag
    22561 ttaaatatca gtggtctggt tgttgataac acctgtgaaa cccgcgttga cggcggtaat
    22621 aaagatggtc tgatcctgct gcaaactgca actgttggtg aaatcgatgc tggcgtgctg
    22681 aatgacaccg ttggcgctaa agccaaacct ttcagcatca ccgttgactg cagcaaagca
    22741 aatccgaatc caggtagtac agctaaaatg accttcggtt ctgttttctt tggtaatagc
    22801 aaaggaacgc tgaacaacga catgtctatt aacaacccgt ctgacggcgt gaatatcgct
    22861 ctgcacaata ttgatggttc cactatcaaa caggttcaaa ttaacaaccc tggcgatgtg
    22921 tatactaaag ccctggatgc aacgacaaaa tctgctgttt atgattttaa agcgtcttac
    22981 gttcgtgccg tcgcagacca aacagcaact gctggttatg taaaaactaa cactgcatac
    23041 accattactt atcagtaagt attcaccaat taaattgatt gaatgaatat acagggaata
    23101 gtgttaataa tttctatttt tatattattc cctgttttaa ttaactctgt cagggatggt
    23161 ttatgttttt taacaccaaa catacaacag ctttatgctt cgtaacctgt atggctttta
    23221 gttcatcgtc tattgcggac attgtcattt cgggtactcg cgtaatatat aaaagcgatc
    23281 aaaaaagtgt caacgtacgt ctggaaaata aagggaataa cccgttgctt gtccagagtt
    23341 ggttagatac tggcgataac aacgctgagc ctggcagtat tacagtccct tttactgcca
    23401 cgccgccagt atcgcgtatt gatgccaaac gtgggcaaac aatcaaatta atgtacacag
    23461 ccagcacctc actgcctaaa gacagagaga gcgtgttctg gtttaacgta ctggaagttc
    23521 caccaaaacc agatgcagaa aaggtcgcga atcaaagtct gctgcaactg gcatttcgca
    23581 cacgtataaa acttttctat cgcccggatg gattgaaggg aaatccctct gaagccccgt
    23641 tagccctgaa gtggttctgg tcaggttcag aaggtaaggc gtcattgcga gtgaccaatc
    23701 caacccctta ctacgtctct tttagcagtg gtgatttaga agctagcggt aaacgctatc
    23761 cgattgatgt gaaaatgatt gcaccattta gtgatgaggt catgaaagtc actggcctta
    23821 atggcaaagc aagttctgca aaagtacatt tttacgccat taatgatttt ggtggcgcca
    23881 ttgaaggtaa tgccagtctg taatcaggca aggatataat tccgcaggaa gcataacgtg
    23941 actataaaac caactaaaaa ttatcataat catctgaccc gtatcgctac gttttgcgcg
    24001 ctgctgtatt gcaatagtgc tttctgtgct gaacttgttg aatatgacca taccttcctg
    24061 atggggcaga atgcctcaaa tattgatctc agccggtata gtgaaggtaa ccctgctata
    24121 ccgggtatgt acgacgtcag tgtttatgtc aacgatcaac ctattattaa ccaaagcatt
    24181 acatttattg aaattgaagg aaaaaaaacg cacaagcctg tatcacactt aagaatttat
    24241 tgcagtttca tataaattct cccgatataa ataacgaaaa agccgttctg cttgccaggg
    24301 atgaaacact cggcaattgc cttaatttga cggaaattat ccctcaggct tctgttcgtt
    24361 atgacgttaa cgagcaacgt ctggatatag acgttcctca ggcatgggta atgaaaaatt
    24421 accaaaacta tgttgatcca tcgttatggg aaaa
//
LOCUS       DAJVQG010000052        23954 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJVQG010000052 DAJVQG010000000
VERSION     DAJVQG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23954
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.052"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(38..400)
                     /locus_tag="OQM83_001863"
     CDS             complement(38..400)
                     /locus_tag="OQM83_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCS1520140.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            937..2850
                     /gene="mtlA"
                     /locus_tag="OQM83_001864"
     CDS             937..2850
                     /gene="mtlA"
                     /locus_tag="OQM83_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCS1520141.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3080..4228
                     /gene="mtlD"
                     /locus_tag="OQM83_001865"
     CDS             3080..4228
                     /gene="mtlD"
                     /locus_tag="OQM83_001865"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCS1520142.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4228..4815
                     /gene="mtlR"
                     /locus_tag="OQM83_001866"
     CDS             4228..4815
                     /gene="mtlR"
                     /locus_tag="OQM83_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCS1520143.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4827..5036)
                     /locus_tag="OQM83_001867"
     CDS             complement(4827..5036)
                     /locus_tag="OQM83_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1520144.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5321..5683
                     /locus_tag="OQM83_001868"
     CDS             5321..5683
                     /locus_tag="OQM83_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCS1520145.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            6226..6904
                     /locus_tag="OQM83_001869"
                     /pseudo
     CDS             6226..6904
                     /locus_tag="OQM83_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
     gene            6948..11798
                     /locus_tag="OQM83_001870"
     CDS             6948..11798
                     /locus_tag="OQM83_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HCS1520146.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTN
                     DNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIA
                     KGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            12166..13821
                     /gene="lldP"
                     /locus_tag="OQM83_001871"
     CDS             12166..13821
                     /gene="lldP"
                     /locus_tag="OQM83_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCS1520147.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            13821..14597
                     /gene="lldR"
                     /locus_tag="OQM83_001872"
     CDS             13821..14597
                     /gene="lldR"
                     /locus_tag="OQM83_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCS1520148.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            14594..15784
                     /gene="lldD"
                     /locus_tag="OQM83_001873"
     CDS             14594..15784
                     /gene="lldD"
                     /locus_tag="OQM83_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCS1520149.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            15980..16453
                     /gene="trmL"
                     /locus_tag="OQM83_001874"
     CDS             15980..16453
                     /gene="trmL"
                     /locus_tag="OQM83_001874"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCS1520150.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(16506..17327)
                     /gene="cysE"
                     /locus_tag="OQM83_001875"
     CDS             complement(16506..17327)
                     /gene="cysE"
                     /locus_tag="OQM83_001875"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCS1520151.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(17407..18426)
                     /gene="gpsA"
                     /locus_tag="OQM83_001876"
     CDS             complement(17407..18426)
                     /gene="gpsA"
                     /locus_tag="OQM83_001876"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCS1520152.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(18426..18893)
                     /gene="secB"
                     /locus_tag="OQM83_001877"
     CDS             complement(18426..18893)
                     /gene="secB"
                     /locus_tag="OQM83_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCS1520153.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(18956..19207)
                     /gene="grxC"
                     /locus_tag="OQM83_001878"
     CDS             complement(18956..19207)
                     /gene="grxC"
                     /locus_tag="OQM83_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCS1520154.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELLIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(19349..19780)
                     /locus_tag="OQM83_001879"
     CDS             complement(19349..19780)
                     /locus_tag="OQM83_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCS1520155.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            20025..21569
                     /gene="gpmM"
                     /locus_tag="OQM83_001880"
                     /gene_synonym="pgmI"
     CDS             20025..21569
                     /gene="gpmM"
                     /locus_tag="OQM83_001880"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCS1520156.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            21579..22862
                     /gene="envC"
                     /locus_tag="OQM83_001881"
     CDS             21579..22862
                     /gene="envC"
                     /locus_tag="OQM83_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCS1520157.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            22866..23825
                     /locus_tag="OQM83_001882"
     CDS             22866..23825
                     /locus_tag="OQM83_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCS1520158.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
BASE COUNT         6048 a         6108 c         6211 g         5587 t
ORIGIN      
        1 agcaccacgt aagttaaaac aatcaataga tccataatta gatctccggg aacgtggaat
       61 agtcagtctt tttctcggcg gcgagttgat gctctagccg ggaaattcgg tcagaaagtg
      121 cggcgatttc cgggtcagtc gctttctcct gcgacgcaac atgtgactgc atgccccacc
      181 cgcgctccgg ttgatagagc gtcgcccaga tccacaggaa cggccagata acatgcaggg
      241 taaacaggct cacccagccc gccgtatgaa tagcgtcggc atggggatgg ttgcgctttt
      301 tggcaatcag ataagggatg tcatgtatcg cgatgattcc ataaaaaatc accagaaaga
      361 cgaagatcag cactcccaac gcgaaatagt ttagaaacat atctgcctcg gattcacgtt
      421 tatcagtgtt gtttttgggc tggcagccag aagggagtca ggctgatatt ttgacaataa
      481 tccgggttcg cgattctcgc cataacacca aagaataatt tttagaggtg atgagttgct
      541 tagttacata acaattgtat gacgaaggca taacatgctg tagatcacat caggtgaacg
      601 ccgtaagaaa atatcttgtg attcagatca caaagattca acaaaccatc aaaacaaaaa
      661 tgtgacacta ctcacattta aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt
      721 tacacaagcg ttttgtgatg aacgtcacgt taattacctc tctaccccct atatttatgt
      781 gattgatatc acacaaaagg ccgtcgactg gacagttaac cgattcagtg ccagatttcg
      841 cagtatctac aaggtccggc tacctctgcc gccacattaa caaaaaacct cgggcttcca
      901 gcctgcgcga cagcaaacat aagaaggggt gtttttatgt catccgatat taagatcaaa
      961 gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc caaatatcgg cgcgtttatc
     1021 gcgtggggta tcatcaccgc gttatttatt ccaacagggt ggttaccgaa cgagacgctg
     1081 gcgaagctgg tcgggccgat gatcacttat ctcctgccgc tgctgatcgg ttataccggt
     1141 ggtaagctgg taggcggcga acgtggcggc gtagtcggtg ccatcaccac catgggcgtt
     1201 atcgtcggcg cagacatgcc gatgttcctc ggttctatga ttgcaggtcc gctgggcggc
     1261 tggtgcatta agcacttcga ccgctgggta gacggtaaga tcaaatccgg ttttgagatg
     1321 ctggtgaata acttctccgc aggcatcatc gggatgatcc tcgctattct ggcattcctc
     1381 ggcattggcc cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg
     1441 gttgtccatg acatgctgcc gctggcgtct atctttgttg aaccggcgaa aatcctgttc
     1501 ctcaacaacg ccattaacca cggtatcttc tcgccgctgg gtattcagca gtcccatgaa
     1561 ctgggtaaat caatcttctt cctgattgaa gctaacccag gtccaggtat gggcgtgctg
     1621 ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc
     1681 atccacttcc tgggtggtat ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt
     1741 ctgatcctcg cagtcatcct cggcggtatg actggcgtgt tcacgctgac tatcctgggc
     1801 ggtggtctgg tttctccggc atctccgggt tctatccttg ctgtactggc gatgacacca
     1861 aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt
     1921 gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat tgaagcagca
     1981 actcgtcgta tgcaggacat gaaagctgag tctaaaggca catctccgct gtctgcgggc
     2041 gatgtgacta acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg
     2101 ggttccagtg cgatgggcgc aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtca
     2161 cagatttcgg tcactaacag cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc
     2221 actcaccgtg acctgaccga acgcgctatg cgccaggttc cgcaggcgca gcatatttcg
     2281 ctgaccaact tcctcgacag cggcctgtac accagcctaa ccgaacgtct ggttgctgcc
     2341 cagcgccata ctgaaaacga agtgaaagtg aaagacagtc tgaaagacag ctttgacgat
     2401 tccagtgcta acctgttcaa actgggcgcg gagaacatct tcctcggtcg caaagcagca
     2461 accaaagaag aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag
     2521 ccggaatacg ttcaggcgat gctggatcgt gaaaaactga ccccgactta tctgggtgag
     2581 tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa aacgggcgta
     2641 gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt
     2701 ctggtgattg gtattgcagc ccgtaacaac gagcacattc aggttatcac cagcctgacc
     2761 aatgcactgg atgatgagtc cgtcatcgag cgtctggcac acaccaccag cgtggatgaa
     2821 gtgctggaac tgttggcagg tcgtaagtaa tccaatccca ccctctccac atggagaagg
     2881 tggggttaat tgcctgatgc gctacgctta tcaggcctac aggatgcatc acgatttgtt
     2941 gaatttgcac gttcttgtag gccggataag gcgcttacgc cgcatccggc gctgcccctc
     3001 tcctcacgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc gggtaaaaac
     3061 attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata tcggtcgtgg
     3121 ctttatcggt aaactgctgg cagacgcggg tatccaactg acgtttgccg atgtcaatca
     3181 ggtggtactt gatgccctga atgcccgtca tagctatcag gttcatgtgg ttggtgaaac
     3241 cgagcaggta gataccgttt ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt
     3301 tgatctgatt gctcaggtcg atttagtcac taccgccgtt ggcccggttg tgctggaacg
     3361 tattgctccg gctatcgcca aagggctggt gaaacgcaaa gaacaaggta atgaatcccc
     3421 gctgaacatc atcgcctgtg aaaacatggt acgcggtacc acgcagctga aaggccatgt
     3481 gatgaacgcc ctgccggaag acgccaaagc gtgggtagaa gaacacgttg gctttgtcga
     3541 ttccgccgtt gaccgcatcg taccgccttc ggcttcggca actaacgatc cgctggaagt
     3601 gacggtagaa actttcagcg aatggattgt cgataaaacg cagttcaaag gcgcactgcc
     3661 gaacatccca ggcatggagt taaccgacaa cctgatggca tttgtcgaac gtaaactctt
     3721 caccctgaac acgggtcatg ctataaccgc gtacctcgga aaactggccg gtcatcagac
     3781 cattcgtgac gctattctcg acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga
     3841 aagtggtgcg gtactgatca agcgctacgg ctttgacgca gacaaacatg cggcgtacat
     3901 ccagaaaatc ctcggtcgtt ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg
     3961 ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccat tgctcggtac
     4021 gctggaatac agccttccgc acaagaatct gattcagggg attgctggtg caatgcactt
     4081 ccgcagtgaa gatgatccgc aggctcagga actggcagca ctgatcgctg acaaaggtcc
     4141 gcaggcggcg ctggcacaga tttccgatct tgatgccaac agcgaggttg tatccgaggc
     4201 ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggacac cctgcgcccg
     4261 aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt
     4321 gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag
     4381 cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga cgattacgcg
     4441 gtgaagtatg ctgtagaacc gttactcgac ggcgacggtc cgctgggcga tctttctgtg
     4501 cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga agatgcggag
     4561 ctgctgatgg cattgcgtga agaactaaat cacgacggca acgagtacgc ctttaccgac
     4621 gacgagatcc ttggaccctt tggtgaactg cactgcgtgg cggcgttacc accgccgcca
     4681 cagtttgaac cagcagactc cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct
     4741 gtgcgatcaa caatggtcct ttcactgact gagctgattt ccagaatcag cttaaaaaaa
     4801 gcctttcaaa agtaagcaac gtctgcttac tgcccctcta cctgcttcgg ccgataaagc
     4861 cgacgataat actccagacg ttgtagatat aacggcacgc tttcatcggg tattccggac
     4921 ggaatcgcgt tacggggagg aagttttttc agatactccc ggaacgcctg gcttgatgcc
     4981 atgaaatcta cggctttatc gataagaagc ggaacgtttt cacctaattt gcccatgatg
     5041 atatctccgt attaccccgc gccgggaatg cgcggccgcc aattttagtt taggttcccg
     5101 gtaaagatga cattaaggaa aacgtgctga atcctcaata atcatccgct gctgtttatt
     5161 atcttttttc tatagatttc ttaattaatc aataaattgc gtagttttta agcatgttaa
     5221 tgattacgtt acagaactat tgcaatgtca tcagttgtga acaaagcacc tggtcgcgca
     5281 tactaggggc tataaattta tctttatcag aagccatcac atgaaagaag tcgaaaaaaa
     5341 cgaaatcaaa cgtctcagcg atcgtctgga cgccatccgc caccagcagg ccgatctgtc
     5401 gctggttgaa gccgcagaca aatatgccga gctggaaaaa gagaaagcca cgctggaagc
     5461 agaaattgct cgcctgcgtg aagttcatag ccaaaaactg agtaaagaag cacagaagct
     5521 gatgaagatg ccgtttcagc gcgcaattac caaaaaagag caggctgata tgggcaagct
     5581 gaagaaaagt gttcgcggac tggtggttgt gcacccaatg accgcgctgg gccgtgaaat
     5641 gggcctgcag gagatgactg gattctctaa gactgcgttt taagcgcctc agacgactgg
     5701 ttcgatcata tcaacccgca tctggcttgc cttagctggt caatgcgggg actctcctgt
     5761 ctactcgcct aattaccaaa caattcactc cctttacgtc gatttaccgc tggttgagat
     5821 tctgaaaatg catttttacc gaaatagcca cctataaaca ataaaaatat atagaagata
     5881 tcatttatta attaacaagc agttacgtct ctttttttcg gatttaattg tgaaatttta
     5941 atcatcatat tcatacaata aattatctgt aaattttttc ccagacatgg tttctttcat
     6001 atttgacatt tattaataat catcattaga atccacgaaa ataatatatt catacaaaca
     6061 cagacatctt ttgaaacgaa tgatacattt ttatgatgaa tgcttaaaac aatatttaat
     6121 cgacacacat cactgtcatt aatgtaaagc aacatagatc accaataata caatggacaa
     6181 ctctaccggc attgatcgct ggtcggagga aataaggatt tttacatgaa cttgaagaag
     6241 atttttttca gtgctgtcac tgtttcagtt ctctgcgcat taacgggatg tgactacatt
     6301 gaagaaggca agccagaaag tagtttattg aaacaacaag aagaacatta aaattgatct
     6361 acttgaaaaa caacaagcac agctaaagag tcaactggaa actattcaaa aacaacaaac
     6421 tggcataata agcagtacaa agactttaac tcacgtaatt aaatccgtta aagatcaaca
     6481 aaacactttt atttttaccg agtttaaccc agcaaaaacg aagtatttca tcctgaacaa
     6541 tggttcggtg gctttagcgg gtcgagtgtt atccattgac gccacagaga atggcagtgt
     6601 tattcatatt tcactggtca acttattaag tacacctatc tcaaatattg gttttaatgc
     6661 gacatggggt ggtgaaaaac ctgtcgatgc caaagagttt gcccggtggc aacaattact
     6721 tttcaacaca tcaatgaaat ccacattgaa attattacca ggtcaatggc aagacattaa
     6781 tttgaccctg aagggtgtat cgcccaataa cctgggatat ctgaagttag ccatcaacat
     6841 ggaaaatatt cagttcgaca atcttccctc tgctgaaaat cggcagaaaa gaagcaaaaa
     6901 ataaatgcag ttagtacgct tataacaagg aatagcgagc atcattaatg aacaaaatat
     6961 ttaaagttat ctggaaccct gcgacaggga attatactgt taccagcgaa acggcaaaaa
     7021 gccgtggcaa gaaatctggg cgcagtaagc tgttaatttc tgcgctggtt gcgggtggaa
     7081 tgttgtcgtc gtttggggca ttggcgaatg ccgggaatga caacggccag ggtgttgatt
     7141 acggtagtgg atcagctggc gacagctggg ttgctatagg caaaggggcg aaagcaaata
     7201 cttttatgaa caccagtggt tccagtactg ctgtgggtta tgacgctata gctgaaggcc
     7261 aatatagctc tgccatcggg tcaaaaaccc atgcgattgg tggtgcatca atggcctttg
     7321 gggttagtgc aatatcagaa ggcgatagaa gtatagcact gggtgcctct tcgtattcat
     7381 tgggccaata ctcaatggcc ctcggccgtt attcaaaagc attgggtaaa ttgtctattg
     7441 ctatggggga ctcttccaaa gcggaaggag caaacgccat tgccctggga aatgccacta
     7501 aagctactga gattatgagt attgctcttg gcgacaccgc caatgcgtca aaagcgtatt
     7561 caatggcgct gggagcaagt agcgtcgcat ctgaagaaaa cgcaattgcc ctggggcgta
     7621 gcagtgtagc tagcggtact gacagcctcg catttggcag acaatcactt gccagcgcag
     7681 cgaacgctat tgcgataggt gctgagaccg aagccgctga aaatgcaact gctattggca
     7741 ataatgcgaa ggcaaaaggg actaatagca tggcaatggg gttcggaagc cttgccgata
     7801 aagtcaatac tatcgcatta ggaaatggca gccaggctct ggcagataat gcaatcgcca
     7861 taggccaggg caacaaagct gatggcgtgg atgccatcgc tctgggtaat ggtagccagt
     7921 cgagaggctt aaacaccatt gccttaggca cagccagtaa tgcaactggt gataagagtc
     7981 ttgcgcttgg tagtaatagc agtgccaacg gtattaactc tgtcgcgctg ggcgcagatt
     8041 ccattgcgga tttagacaat accgtctctg tcggcaatag ttcattaaaa cgcaagatcg
     8101 ttaatgtgaa aaatggcgcg atcaagtctg acagttacga tgccattaat ggttcacagc
     8161 tttatgccat tagcgactcg gtagcaaaaa ggcttggagg aggggctgca gtagatgttg
     8221 atgacggtac tgttacagca ccaacctaca atttaaaaaa tggtagcaaa aataacgtag
     8281 gggctgcgct cgctgtactt gatgaaaaca ccctgcaatg ggaccaaacc aaaggcaaat
     8341 acagcgctgc tcatggtact agtagcccaa ctgccagcgt aatcaccgat gttgcggatg
     8401 gcacgatttc agcctccagt aaggatgcgg ttaacggttc ccaactgaaa gctaccaatg
     8461 acgatgtcga agccaacacc gccaatatcg ctactaatac cagcaacatt gccacgaata
     8521 cagcaagtat tgccaccaat accaccaata tcaccaacct gacggattcc gttggtgacc
     8581 ttcaggctga tgccctgctc tggaacgaaa ctaaaaaggc attcagtgca gctcacggcc
     8641 aggataccac cagcaaaatc accaacgtta aagatgccga cctgacggct gacagcactg
     8701 atgctgttaa cggctctcag ctgaaaacca ccaacgatgc tgtggcgacg aataccacca
     8761 atatcgccaa taacacttcc aatattgcca ctaacaccac caacatctct aatctgactg
     8821 agacggtgac taatcttggt gaggatgcgc tgaaatggga taaggacaat ggtgtattca
     8881 cggcagctca tggcaccgag accaccagca aaatcaccaa cgttaaagat ggcgacctga
     8941 cgactggcag caccgatgcc gttaacggct ctcagctgaa aaccaccaac gatgccgtgg
     9001 cgacgaatac caccaatatc gccactaaca ccaccaacat ctctaatctg actgagacgg
     9061 tgactaatct tggtgaggat gcgctgaaat gggataagga caatggtgtc ttcactgcag
     9121 ctcatggcaa caataccgcc agcaaaatca ccaatatcct ggacggcaca gtcactgcaa
     9181 ccagttccga tgccattaac ggtagccagc tttatgactt aagcagcaat atcgccacct
     9241 acttcggcgg caatgcttct gtgaatactg acggtgtgtt taccggtcca acctacaaaa
     9301 tcggtgaaac aaattattat aacgtcggcg atgcactggc tgcgattaac tcctcattta
     9361 gcacgtctct cggcgatgct ctgctttggg atgccaccgc aggtaaattc agtgccaaac
     9421 acggtactaa tggtgacgca agcgtgatca ctgatgtcgc agatggtgaa atttcagact
     9481 ccagttctga cgcagtaaac ggctcacaac tccacggcgt gagcagttat gttgttgatg
     9541 cgctgggggg tggtgccgaa gtcaatgcag acggcaccat cactgcgccg acgtacacca
     9601 ttgctaatgc tgattacgat aatgtcggtg atgccctgaa tgctatcgat accactcttg
     9661 acgacgctct gctctgggat gcggacgccg gtgaaaatgg tgcatttagc gccgctcacg
     9721 gaaaagataa aactgccagt gtaatcacta acgtcgctaa cggtgcaatc tctgctgcca
     9781 gcagcgacgc gattaacggc tcacaactct ataccaccaa taagtacatc gctgatgcgc
     9841 tgggtggtga cgcagaagtc aacgctgacg gcaccatcac cgcaccgact tacaccattg
     9901 cgaacgccga gtacaacaac gtcggtgacg ccctggatgc gcttgatgat aacgccctgc
     9961 tgtgggatga gactgccaat ggcggtgctg gagcctacaa tgccagccat gacggtaaag
    10021 ccagcatcat cactaatgtc gctaatggca gtattagtga ggacagtacc gatgcagtga
    10081 acggttctca gttgaatgcg acgaatatga tgattgagca gaacacccaa attatcaatc
    10141 agctcgctgg taacaccgac gcaacctata tcgaagaaaa tggcgctggt atcaactacg
    10201 ttcgtactaa cgacaacgat ttagccttta acgatgcaag cgcctctggt gtcggcgcta
    10261 cagctgtagg ttataacgct gtcgcgtctg gtgccagcag cgtagccatt ggtcagaaca
    10321 gcagcagcac cgttgatacc ggtattgcgc tgggtagcag ctccgtttcc agccgtgtga
    10381 ttgccaaagg ttctcgtgac actagcgtaa cggaaaatgg cgtggtgatt ggttatgaca
    10441 ccactgacgg cgaactgcta ggtgcattgt caattggtga tgacggtaaa taccgccaaa
    10501 tcatcaacgt agctgatggt tcagaagccc atgacgccgt tacggttcgc cagttgcaga
    10561 acgctattgg agcggtcgcc actacgccaa ccaagtactt tcacgccaac tcaacggcag
    10621 aagactcact ggccgttggt gaagactcgc tggcaatggg tgcgaaaact gtcgttaatg
    10681 gtaatgcagg tattggtatc ggtttgaaca ctctggttct agctgatgcg atcaacggca
    10741 ttgctatcgg cagtaacgca cgcgcaaacc atgcaaacag tatcgcaatg ggtaacggtt
    10801 ctcagaccac ccgtggtgca cagactggct acaccgccta caacatggac gcaccgcaga
    10861 actctgtagg tgagttttct gtcggtagtg aagacggtca acgtcagatc acaaacgtcg
    10921 cagctggttc agcggatacc gatgcggtta acgtgggtca gttgaaagtc actgatgagc
    10981 gcgtagcgca aaatacccag agcattacta acctgaacaa tcaggtcact aatctggata
    11041 ctcgcgttac taatatcgaa aacggtattg gcgacattgt caccaccggt agcaccaagt
    11101 acttcaagac caacaccgat ggcgtagatg ccaacgccca gggtaaagat agcgttgcta
    11161 ttggttctgg ttccattgct gccgctgaca acagcgtcgc actgggtacc ggttccgttg
    11221 cagaggaaga aaatacaatc tctgtaggtt cttctactaa ccaacgccgt atcaccaacg
    11281 tagctgccgg tgttaatgcc accgatgcgg ttaacgtgtc gcagctgaaa tcttctgaag
    11341 caggcggcgt gcgctacgac accaaagctg atggttctat cgactatagc aatatcaccc
    11401 tcggtggtgg caacggcagt acgactcgta tcagcaacgt ctccgctggc gtcaacaaca
    11461 acgacgcggt gaactacgcg cagttgaagc aaagcgtgca ggaaacgaag caatacaccg
    11521 atcagcggat ggttgagatg gataacaaac tgtctaaaac tgaaagcaag ttgagcggtg
    11581 gtatcgcttc tgcaatggca atgaccggtc tgccgcaggc ttatacaccg ggtgccagca
    11641 tggcttctat tggtggcggt acttacaacg gtgaatcggc agttgcttta ggtgtatcga
    11701 tggtgagcgc caatggtcgt tgggtctaca aattacaagg tagtaccaat agccagggtg
    11761 aatactccgc cgcactcggt gccggtattc agtggtaatg atccattaag ttagtgtgac
    11821 taagggcaaa cgccaaattg cctgatgcgt tacgcgcatc aggcaagata aggcgccccg
    11881 ggtcaacaat ctaagggcaa ttctctgatg aggattgccc ttttctttac cagacatctc
    11941 cccccacaag aattggccct accaattctt cgcttatctg acctctggtt cacaattccc
    12001 caattaaaac tcacatcaat attgccaata cataacattt agttaaccat tcattgtcat
    12061 tatccctaca caacacaatt ggcagtgcca cttttacaca acgtgtgaca aggagatgag
    12121 caacagactc attacacgat gtgcgtggac tccaggagac ctgcaatgaa tctctggcaa
    12181 caaaactacg atcccgccgg gaatatctgg ctttccagtc tgatagcatc gcttcccatc
    12241 ctgtttttct tctttgcgct gattaagctc aaactgaaag gatacgtcgc cgcctcatgg
    12301 acggtagcaa tcgcccttgc cgtggctttg ctgttctata aaatgccggt cgctaacgcg
    12361 ctggcctcgg tggtttatgg cttcttctat ggtttgtggc ccatcgcgtg gatcattatt
    12421 gcagcagtgt tcgtctataa gatctcggtg aaaaccgggc agtttgacat cattcgctcg
    12481 tctattcttt cgataacccc tgaccagcgt ctgcaaatgc tgatcgtcgg tttctgtttc
    12541 ggcgctttcc ttgaaggagc cgcaggcttt ggcgcaccgg tagcaattac cgccgcattg
    12601 ctggtcggcc tgggttttaa accgctgtac gccgccggac tgtgcctgat tgttaacacc
    12661 gcgccagtgg catttggcgc gatgggcatt ccaatcctgg ttgccggaca agtaacaggc
    12721 atcgacagct ttgagattgg ccagatggtg gggcggcagc taccgtttat gaccattatc
    12781 gtgctgttct ggatcatggc gattatggac ggctggcgcg gtatcaagga gacgtggcct
    12841 gcggtcgtgg ttgcgggcgg ctcgtttgcc atcgctcagt accttagctc taacttcatt
    12901 gggccggagc tgccggacat tatctcttcg ctggtatcac tgctctgcct gacgctgttc
    12961 ctcaaacgct ggcagccagt gcgcgtattc cgttttggtg atttgggggc gtcaaagatt
    13021 gatatgacgc tggcccacac cggttacact gcgggtcagg tgttacgtgc ctggacaccg
    13081 ttcctgttcc tgacagctac cgtaacactg tggagcatcc cgccgtttaa agccctgttc
    13141 gcatcgggtg gtgcgctgta tgagtgggtg atcaacattc cggtgccgta cctcgataaa
    13201 ctggttgccc gtatgccacc agtggtcagc gaggctacag cctatgccgc cgtgtttaag
    13261 tttgactggt tctctgccac cggcaccgcc attctgtttg ctgcactgct ctcgattgtc
    13321 tggctgaaga tgaaaccgtc tgacgctatc agcaccttcg gcagcacgct gaaagaactg
    13381 gctctaccca tctactccat cggcatggtg ctggcgttcg cctttatctc gaactattcc
    13441 ggactgtcat caacactggc actggcactg gcgcacaccg gtcatgcatt caccttcttc
    13501 tcgccgttcc tcggctggct gggggtattc ctgaccgggt cggatacctc atctaacgcc
    13561 ctgttcgccg cgctgcaagc caccgcagca caacaaattg gcgtctctga tctgttgctg
    13621 gttgccgcca ataccaccgg tggcgtcacc ggtaagatga tctccccgca atctatcgct
    13681 atcgcctgtg cggcggtagg cctggtgggc aaagagtctg atttgttccg ctttactgtc
    13741 aaacacagcc tgatcttcac ctgtatggtg ggcgtgatca ccacgcttca ggcttatgtc
    13801 ttaacgtgga tgattcctta atgattgttt tacccagacg cctgtcagac gaggttgccg
    13861 atcgtgtgcg ggcgctgatt gatgaaaaaa acctggaagc gggcatgaag ttgcccgctg
    13921 agcgccaact ggcgatgcaa ctcggcgtat cacgtaattc actgcgcgag gcgctggcaa
    13981 aactggtgag cgaaggcgtg ctgctcagtc gacgcggcgg cgggacgttt attcgctggc
    14041 gtcatgacac atggtcggag caaaacatcg tccagccgct aaaaacgctg atggccgatg
    14101 atccggatta cagtttcgat attctggaag cccgctacgc cattgaagcc agcaccgcat
    14161 ggcatgcggc aatgcgcgcc acacctggcg acaaagaaaa gattcagctt tgctttgaag
    14221 caacgctaag tgaagacccg gatatcgcct cacaagcgga cgttcgtttt catctggcga
    14281 ttgccgaagc ctcacataac atcgtgctgc tgcaaaccat gcgcggtttc ttcgatgtcc
    14341 tgcaatcctc agtgaagcat agccgtcagc ggatgtatct ggtgccaccg gttttttcac
    14401 aactgaccga acaacatcag gctgtcattg acgccatttt tgctggtgat gccgacgggg
    14461 cgcgtaaagc aatgatggcg caccttagtt ttgttcacac caccatgaaa cgattcgatg
    14521 aagatcaggc tcgccacgca cggattaccc gcctgcccgg tgagcataat gagcattcga
    14581 gggagaaaaa cgcatgatta tttccgcagc cagcgattat cgcgccgcag cgcaacgcat
    14641 tctgccgccg ttcctgttcc actatatgga tggtggtgca tattctgaat acacgctgcg
    14701 ccgcaacgtg gaagatttgt cagaagtggc actgcgccag cgtattctga aaaacatgtc
    14761 cgacttaagc ctggaaacga cgctgtttaa tgagaaattg tcgatgccgg tagcactggc
    14821 accggtgggt ttgtgtggca tgtatgcgcg tcgtggcgaa gttcaggcag ccaaagcggc
    14881 ggacgcgcat ggcattccgt ttactctctc gacggtttcc gtttgcccga ttgaagaagt
    14941 cgcgccagcc atcaagcgcc caatgtggtt ccagctttat gtactgcgcg atcgcggctt
    15001 tatgcgtaac gcgctggagc gagcaaaagc agcgggttgt tcgacgctgg ttttcaccgt
    15061 ggatatgccg acaccgggcg cacgctaccg tgatgcgcat tcaggtatga gcggcccaaa
    15121 tgcggcaatg cgccgctact tgcaggcggt gacgcatccg caatgggcgt gggatgtggg
    15181 cctgaacggt cgtccacatg atttaggtaa tatctcagct tatctcggca aaccgaccgg
    15241 actggaagat tacatcggct ggctggggaa taacttcgat ccgtccatct catggaaaga
    15301 ccttgagtgg atccgcgatt tctgggatgg cccgatggtg atcaaaggga tcctcgatcc
    15361 ggaagatgcg cgcgatgcag tacgttttgg tgctgatgga attgtggttt ctaaccacgg
    15421 tggccgccag ctggacggtg tactctcttc cgctcgtgcg ttgcccgcta ttgcagatgc
    15481 ggtgaaaggt gatatcgcca ttctggcgga tagcggaatt cgtaacgggc ttgatgtcgt
    15541 gcgtatgatt gcgctcggtg ccgacaccgt actgctgggt cgtgctttcc tgtatgcgct
    15601 ggcaacagcg ggccaggcgg gtgtagctaa cctgctaaat ctgatcgaaa aagagatgaa
    15661 agtggcgatg acgctgactg gcgcgaaatc gatcagtgaa attacgcaag attcgctggt
    15721 gcaggggctg ggtaaagagt tgcctacggc actggctccg atggcgaaag ggaatgcggc
    15781 atagtcgttt gcgccccctc accctaaccc tctccctcag ggagagggga ccgttcggcg
    15841 ctgtatgtac tccctcactc tgaaacgaca ccgcactctt tttttctccc tcgcccctcc
    15901 ggggagggcc ggggtgaggg gaaaaggccg cacggttcac caattttctg ctatcctgcc
    15961 cccgcactaa gggggcagta tgctaaacat cgtactttac gaaccagaaa ttccgccaaa
    16021 tactggcaac atcatccgtc tttgcgctaa taccggcttt cgtctgcata tcatcgaacc
    16081 gatgggattt gcctgggacg ataagcgcct gcgccgcgcg gggctggact atcacgagtt
    16141 taccgccgtt acgcgtcatc atgactatcg cgcgttcctc gaagcagaaa atccccagcg
    16201 cctgtttgcc ctcaccacga aaggtacgcc tgctcacagc gccgtaagct atcaggatgg
    16261 cgattatctg atgttcggcc cggaaacacg cggcctgcca gcgagcattc ttgatgccct
    16321 gcccgctgaa caaaaaattc gcattccgat ggtgccggac agccgcagca tgaatctgtc
    16381 caatgcggtg tcggtagtgg tgtatgaagc ctggcggcag ctgggatatc cgggagcgtt
    16441 gttgagagat tagttactgt atgccggata gatgattaca tcgcatccgg cacgatcaca
    16501 ggacattaga tcccatcccc atactcaaat gtatggttaa taccgttgaa atgctggtcc
    16561 atatccattg atggcttatc gctgtctggt ttaccgacaa tacgagccgg aacgccagcg
    16621 gcggttgtat gcggcggcac cggttgcagc accacggaac ctgcgccaat cttcgcgccg
    16681 cgaccaactt caatattgcc gaggattttc gcgcccgcgc caatcatcac accttcacga
    16741 attttcgggt gacggtcacc accagattta cccgtaccgc caagcgtcac agattgcaga
    16801 atcgatacgt cgttttcaat taccgccgtt tcacccacaa cgatgcccgt ggcgtggtcg
    16861 agcataatgc cacggccaat ttttgctgcc gggtgaatat cgacctggaa cgtcacagaa
    16921 acctggtttt gcagaaagat tgccaatgcg cgacgcccct gattccacaa ccagtgaccg
    16981 atgcgatagg cctgcaaggc atgaaaaccc ttcaggtata acaacggggt tgagtattta
    17041 tcgactgccg ggtcgcgggt acgcaccgcc tgaatatcac aggccgcaga ggcgatcatt
    17101 tccgggtcag cggcgtaggc ttcttccacc acttcacgga tagcaatagc aggcataatt
    17161 ggcgatgaca gcttgttcgc cagcatgtag ctcagtgcac taccaaggtt ttcgtgcttg
    17221 agtagcgtcg cgtggtaaaa actggccagc attggctcac agtccgccag cgttctggct
    17281 tcggctttaa tattgttcca gacaatttcc agttcttcac acgacattgc ttactccaca
    17341 cgatgagata atgaccggcc cgttctgcgc gggccgggtc atagcggtaa caaaggttcc
    17401 ctggggttag tggctgctgc gctcgtcctt gcgtgcacga cctaataacg tcaatgctgc
    17461 ctcgcgcgcg ttttttccgc aatataatac ttgataaatt tcctcggtta ttggcatttc
    17521 tacgccgaag cgatgcgcca gttcgcggac ttctttcgta ttgcggtagc cttccaccac
    17581 ctgaccaatc ttctcctgcg cgctttgtac atccatgccc tgaccgagca tcatgccaaa
    17641 acggcggtta cgcgactggt tgtcggtaca ggtaagcacc agatcgccca gccccgccat
    17701 gcccataaag gtggcaggat cggcacccag cgccgcgcca agacgcgaca tttcagccag
    17761 cccacgggtg atcagtgccg tacgcgcatt cgcaccaaaa ccgataccgt cggacatccc
    17821 cgcgccaatg gcaatgacgt ttttcaccgc accgccaagc tgcacaccga tgaaatccgg
    17881 gttgctgtaa acgcggaaac ttttgccaca gtgcagcaat tgttggagat catcggcaaa
    17941 ggtctggtcg gtagaggcca acgaaatagc cgtcggtaaa cctgccgcca gttctttcgc
    18001 aaacgttggg ccagagataa ccgccagcgg aatttgatcg cctaaggcct cacgcgccac
    18061 gtcctgtaac agacgtccgg tttccgcttc cagccctttg gtcgcccaca ccagacgcgc
    18121 atcaggacgc atcagcggtt taatctggcg cagcacttca ccaaagacat ggctgggtac
    18181 gacgacgaga atattacggc tggctgccag cgcagtggcg agatcgcttt caagatggag
    18241 cgtatcggga aaaggcacat cggggagaaa cgcggcgtta cagcggtcgc gttcaagcgt
    18301 tgcgatatgt tcagggtcat ggccccagag gacaacctcg tggccatttc ttgccagggt
    18361 aatggcaaga gcggtgccgt acgagccggc accgatcaca gtcattgaag cattacgttg
    18421 gttcatcagg catcctgatg ttcttcagta ccttcgccag cctgctgctg caaatagttc
    18481 atgaacagcg catcgaagtt aatcggcgca aggttcagtt gcgggaatgt accgcgggat
    18541 accacgctgg tgatgcactc acgagcatac gggaacagaa tgttcgggca gtatgcgccc
    18601 aggcaatgtg ccatctgggt gccttcgata cccgcgatgg agaaaatacc gccctgctga
    18661 acttcacaca ggaacgcggt ttcttcgccc agggaggccg ttacggtaac acgcagtacc
    18721 acttcgtata cgtcatctgc cagttgggaa gatgccgtat ccagatcaag tttaacttct
    18781 ggttgccaat ctttctggaa aacgtgcggc gcgttcggcg cttcgaaaga gatatccttg
    18841 gtataaatac gttggatctg gaaagtcatt tcagtgttgt tttgttctga catgtgtaga
    18901 aaacccttaa gtgttgtcct taaatactgc gcaatgtaat gccaacgttc acacgttatt
    18961 tcagcagggg atccagtcca ccacgtgcat ccaatgcata caagtcatca cagccgccaa
    19021 tgtgctgtgc gtcaataaaa atctggggaa ccgtggtgcg accgctgcgt ttgatcatct
    19081 cttcacgctt ggcggcattg ccatcgatca gcaactcctg aaaactcacg cccttgctgc
    19141 tcagcagtgc ttttgcacga tggcaatacg ggcaggtttc tttggtatag atttcaacat
    19201 tggccataat ttagctcctg tttttttacc ctgaaatttc atgttgcagg gcggctgcaa
    19261 aatggagtgt ccccagaagc ttataacaat aagtcgctgg gggcctaatt gcagctaacg
    19321 gcctgcatca tgaaagacga caggtaaatt atttgccgcg caccagaggc aagttttcgc
    19381 cagcccagcc agcaacgccc tctttcagca cgaatacttg cgcaaaacca gctttcgtca
    19441 gcgcgtttgc aggctcctgg cactgcatgc cagaaccgtc taccacgata accggtttgt
    19501 ctttgtgctt ctcaagctca ccaacattgt tggctttgat ttcgctcggc aacagattga
    19561 tagaacctgc gatatggcct ttacggaagt catcacgctg acgtaaatcc acaaccacag
    19621 cgtcttcttt gttgatcaga cgcgtagctt caccacgagt aatcaccttc actttcgagg
    19681 tcaggctctt aaacgtagtc acaagaaccg ccaccagtaa cgcgatccag gcgatactca
    19741 gtatgggatg acggccaaca aattgcataa tttcttgcat ggggggtaac aactcccgac
    19801 gtagtgatta aaaaaaccag gaaaggagta tacctgcgcg gtgcggcaaa tacagccagc
    19861 gcgttaactg gaatgcaatt ttgcggggca cgacgaaaaa aaagcgcaaa tctggcatcg
    19921 gcttgccgcg agcggtcgta tattttgatc ttcagaggct attttatcga ttcagctgta
    19981 gtaaaattac gcaaattttg actcttgagt atgaggttgt cgcaatgtcg gtttctaaaa
    20041 aacctatggt actggtgatt ctggatggat atggctatcg tgaagaacag caggataacg
    20101 ccatttttag tgctaaaacc ccggtaatgg atgcactgtg ggccaatcgt ccgcataccc
    20161 tgatcgacgc ttccggtctg gaagtcggtc tgcctgaccg tcagatgggt aactccgaag
    20221 taggccacgt taacctgggt gccggccgca tcgtgtatca ggacctgact cgtctggacg
    20281 ttgaaatcaa agatcgcgct ttctttgcta atccagtgct gactggcgcg gtagataaag
    20341 cgaaaaacgc aggtaaagca gtacacatta tgggtctgct ctccgcaggc ggcgtacaca
    20401 gccacgaaga tcacatcatg gcgatggtag aactggcagc tgaacgcggc gcagaaaaaa
    20461 tctacctgca cgcattcctt gacggtcgcg acaccccgcc gcgcagtgct gaatcctcgc
    20521 tgaaaaaatt tgaagagaaa tttgctgcgc tgggtaaagg ccgcgtagcg tccatcattg
    20581 gtcgttacta cgcgatggac cgcgataacc gttgggatcg cgttgaaaaa gcctatgacc
    20641 tgctgactct ggcgcagggt gagtttcagg ccgatactgc cgttgctggt ttgcaggcag
    20701 cttatgctcg cgacgaaaac gacgagttcg tgaaagcgac cgttattcgt gcagaaggtc
    20761 agccagatgc ggcaatggaa gacggcgatg cgctgatttt catgaacttc cgtgctgacc
    20821 gcgcgcgtga aatcactcgt gcattcgtga acgctgattt cgatggcttc gcgcgtaaga
    20881 aagtggttaa cgtcgatttc gtgatgctga ccgaatacgc cgctgacatc aaaactgcgg
    20941 ttgcttaccc acccgcttcc ctggttaaca ccttcggcga gtggatggcg aaaaacgaca
    21001 aaactcagtt gcgtatttcc gaaaccgaaa aatatgccca cgttactttc ttcttcaacg
    21061 gtggcgtaga agagtcgttc aaaggcgaag atcgcattct gatcaactca ccgaaagtgg
    21121 ctacctacga tctgcaaccg gaaatgagct ccgcagagct gaccgaaaaa ctggttgcgg
    21181 ccatcaagag cggcaaatac gacaccatta tctgtaacta tccgaacggc gacatggtag
    21241 gtcacaccgg ggtgatggaa gcggcggtta aagcggttga agcactggat cactgcgtgg
    21301 aagaagtcgc gaaagcggtt gagtccgtgg gtggacaact gctgatcacc gctgaccacg
    21361 gtaacgctga acagatgcgc gatccggcaa cgggtcaggc acacacggca cacaccaacc
    21421 tgccagttcc gctgatttac gttggtgata agaacgtgaa agcggttgca ggcggcaaac
    21481 tttctgacat cgcgccgacc atgttgtcgc tgatgggtat ggaaatcccg caagagatga
    21541 ctggtaagcc gctgttcatc gtggaataat ccctccccat gaggggaaag gcgattaata
    21601 ccatgacacg ggccgtgaaa ccgcgcaggt ttgcaatcag gcccatcatc tacgccagcg
    21661 ttcttagcgc tggcgtattg ttgtgcgcct tttccgccca cgcggatgag cgtgaccaac
    21721 tcaaatctat tcaggccgat atcgccacaa aagagcgcgc ggtacgccaa aagcaacaac
    21781 aacgcgcaag cctgctcgca caattgaaaa agcaggaaga agcgatctct gaagccaccc
    21841 gtaagctgcg cgaaacgcaa aacacgctca atcaactcaa taaacagatt gatgagatga
    21901 acgcgtcgat tgccaaactg gagcagcaaa aagccgccca ggagcgcagc ctcgccgcac
    21961 aactggatgc cgcattccgt cagggcgagc ataccggtat tcagctaatt ctcagcggtg
    22021 aagaaagcca gcgtggacag cgtttacagg cttatttcgg ctatctcaac caggcgcgac
    22081 aagaaaccat tgcccagttg aagcaaacgc gtgaagaagt cgccatgcag cgtgctgaac
    22141 tggaagagaa acaaagcgag caacaaacgc tgttatatga acagcgcgcc caacaggcga
    22201 aactgactca ggctctgaac gagcgtaaaa agacgctggc ggggctggaa tcttccatcc
    22261 agcaaggtca gcagcagcta agtgaactgc gtgccaacga atcccgtctg cgtaacagta
    22321 ttgcccgtgc ggaagccgcg gcgaaagcgc gtgctgaacg agaagcgcgt gaggctcagg
    22381 cagttcgcga caggcagaaa gaagcgacgc gcaaaggcac cacctacaaa ccgaccgaaa
    22441 gcgaaaaatc gctgatgtcc cgaactggtg gcctgggtgc gccgcgtggt caggcattct
    22501 ggccagttcg tggccctacg ctgcatcgct atggcgagca gctacagggt gaattacgct
    22561 ggaaaggaat ggttatcggt gcctctgaag gtactgaagt taaagcaatt gccgacggtc
    22621 gggtgattct ggctgactgg ctgcaaggct acggtctggt ggtggtggtt gagcacggta
    22681 aaggcgatat gagtctttac ggctataatc aaagcgcgct ggtgagcgtt ggttcgcagg
    22741 ttcgtgcggg ccagccaatt gcactggtgg gcagcagtgg cggtcagggc agaccttcac
    22801 tctatttcga aattcgccgc cagggtcagg ccgtcaatcc acagccgtgg ttgggaagat
    22861 aagttttgtt tccatttcgt cgtaacgttc ttgcatttgc cgctctgttg gcgctctcct
    22921 cccctgtaat tgctggcaaa cttgccatcg tcattgatga ttttgggtat cgcccgaaca
    22981 acgaaaacca ggtgctggcg atgccttccg ctatctccgt cgctgtatta cccgattcac
    23041 cgcacgccag agagatggcg accaaagcgc ataacagcgg tcacgaagtg ttgattcatc
    23101 tcccgatggc accgttgagt aaacagccgc tggagaaaaa tacgctgcgc ccggagatga
    23161 gcagcgacga aattgagcgc attattcgta gtgcggtcaa taacgtgccc tatgccgtgg
    23221 ggatcaataa ccacatgggc agcaagatga cctctaacct gtttggtatg cagaaagtga
    23281 tgcaggcgct ggagcgttat aatctttact tcctcgacag cgtaaccatt ggtaataccc
    23341 aggcgatgcg cgccgcgcaa ggcactggcg tgaaggtgat caaacggaag gtgttcctcg
    23401 acgattcaca gaacgaagcg gacatccgtg tgcaattcaa tcgcgcaatt gacctagcgc
    23461 gtcgcaacgg atcaaccatt gccattgggc atcctcaccc ttcaacggta cgcgtgttgc
    23521 aacagatggt ttataacctg ccgccagaca ttacgctggt gaaagccagc agcttgctga
    23581 atgaaccgca ggttgatacg tctacaccgc cgaaaaacac tgtgcccgac acaccgcgta
    23641 atccgttccg tggcgtgaag ctgtgcaaac cgaagaaacc gatagaacct gtttatgcta
    23701 atcgcttctt tgaagtatta agcgaaagca tcagccagag cacgctgatc gtttacttcc
    23761 agcatcagtg gcaaggctgg ggcaaacagc ctgaagcggc gaagcttaac gctagcgtaa
    23821 attaaaggcg ctacgcgtaa tgcgatggtg cgaccgcgtt ttgtcgcgcc attgccataa
    23881 gcggaaagac tacagtaacg cctgataacc cacctttacg ctgcgcacat tggtaatcag
    23941 gcgcttatac atac
//
LOCUS       DAJVQG010000053        23948 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJVQG010000053 DAJVQG010000000
VERSION     DAJVQG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23948
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.053"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(507..1304)
                     /locus_tag="OQM83_001883"
     CDS             complement(507..1304)
                     /locus_tag="OQM83_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1520159.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNSELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(1314..1865)
                     /gene="ybcL"
                     /locus_tag="OQM83_001884"
     CDS             complement(1314..1865)
                     /gene="ybcL"
                     /locus_tag="OQM83_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCS1520160.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(2034..2366)
                     /gene="emrE"
                     /locus_tag="OQM83_001885"
     CDS             complement(2034..2366)
                     /gene="emrE"
                     /locus_tag="OQM83_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCS1520161.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(2700..3014)
                     /gene="fliE"
                     /locus_tag="OQM83_001886"
     CDS             complement(2700..3014)
                     /gene="fliE"
                     /locus_tag="OQM83_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCS1520162.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            3230..4888
                     /gene="fliF"
                     /locus_tag="OQM83_001887"
     CDS             3230..4888
                     /gene="fliF"
                     /locus_tag="OQM83_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCS1520163.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            4881..5876
                     /gene="fliG"
                     /locus_tag="OQM83_001888"
     CDS             4881..5876
                     /gene="fliG"
                     /locus_tag="OQM83_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCS1520164.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5869..6555
                     /gene="fliH"
                     /locus_tag="OQM83_001889"
     CDS             5869..6555
                     /gene="fliH"
                     /locus_tag="OQM83_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCS1520165.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            6555..7928
                     /gene="fliI"
                     /locus_tag="OQM83_001890"
     CDS             6555..7928
                     /gene="fliI"
                     /locus_tag="OQM83_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCS1520166.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            7947..8390
                     /gene="fliJ"
                     /locus_tag="OQM83_001891"
     CDS             7947..8390
                     /gene="fliJ"
                     /locus_tag="OQM83_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCS1520167.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            8387..9514
                     /gene="fliK"
                     /locus_tag="OQM83_001892"
     CDS             8387..9514
                     /gene="fliK"
                     /locus_tag="OQM83_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCS1520168.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            9619..10083
                     /gene="fliL"
                     /locus_tag="OQM83_001893"
     CDS             9619..10083
                     /gene="fliL"
                     /locus_tag="OQM83_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCS1520169.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            10088..11092
                     /gene="fliM"
                     /locus_tag="OQM83_001894"
     CDS             10088..11092
                     /gene="fliM"
                     /locus_tag="OQM83_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCS1520170.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            11089..11502
                     /gene="fliN"
                     /locus_tag="OQM83_001895"
     CDS             11089..11502
                     /gene="fliN"
                     /locus_tag="OQM83_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCS1520171.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLISQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            11505..11870
                     /gene="fliO"
                     /locus_tag="OQM83_001896"
     CDS             11505..11870
                     /gene="fliO"
                     /locus_tag="OQM83_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCS1520172.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            11870..12607
                     /gene="fliP"
                     /locus_tag="OQM83_001897"
     CDS             11870..12607
                     /gene="fliP"
                     /locus_tag="OQM83_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCS1520173.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            12617..12886
                     /gene="fliQ"
                     /locus_tag="OQM83_001898"
     CDS             12617..12886
                     /gene="fliQ"
                     /locus_tag="OQM83_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCS1520174.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12895..13680
                     /gene="fliR"
                     /locus_tag="OQM83_001899"
     CDS             12895..13680
                     /gene="fliR"
                     /locus_tag="OQM83_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCS1520175.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            13965..14588
                     /gene="rcsA"
                     /locus_tag="OQM83_001900"
     CDS             13965..14588
                     /gene="rcsA"
                     /locus_tag="OQM83_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCS1520176.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKKLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(14632..14820)
                     /gene="dsrB"
                     /locus_tag="OQM83_001901"
     CDS             complement(14632..14820)
                     /gene="dsrB"
                     /locus_tag="OQM83_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCS1520177.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            14983..15210
                     /gene="yodD"
                     /locus_tag="OQM83_001902"
     CDS             14983..15210
                     /gene="yodD"
                     /locus_tag="OQM83_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCS1520178.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            15508..16323
                     /locus_tag="OQM83_001903"
     CDS             15508..16323
                     /locus_tag="OQM83_001903"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HCS1520179.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(16320..18014)
                     /gene="yedQ"
                     /locus_tag="OQM83_001904"
     CDS             complement(16320..18014)
                     /gene="yedQ"
                     /locus_tag="OQM83_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HCS1520180.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(18185..18367)
                     /locus_tag="OQM83_001905"
     CDS             complement(18185..18367)
                     /locus_tag="OQM83_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCS1520181.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(18446..19363)
                     /locus_tag="OQM83_001906"
     CDS             complement(18446..19363)
                     /locus_tag="OQM83_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCS1520182.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            19536..20457
                     /gene="yedA"
                     /locus_tag="OQM83_001907"
                     /pseudo
     CDS             19536..20457
                     /gene="yedA"
                     /locus_tag="OQM83_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            complement(20446..20916)
                     /gene="vsr"
                     /locus_tag="OQM83_001908"
     CDS             complement(20446..20916)
                     /gene="vsr"
                     /locus_tag="OQM83_001908"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCS1520183.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(20897..22315)
                     /gene="dcm"
                     /locus_tag="OQM83_001909"
     CDS             complement(20897..22315)
                     /gene="dcm"
                     /locus_tag="OQM83_001909"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCS1520184.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(22382..23038)
                     /locus_tag="OQM83_001910"
     CDS             complement(22382..23038)
                     /locus_tag="OQM83_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCS1520185.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            complement(23078..23443)
                     /locus_tag="OQM83_001911"
     CDS             complement(23078..23443)
                     /locus_tag="OQM83_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS1520186.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
BASE COUNT         5983 a         6180 c         5923 g         5862 t
ORIGIN      
        1 gatctacttc acaatttaaa tgagaaacta aaacttacat cttgaaataa tctcattgat
       61 tggatgaata tttgtagtgc agtgacatca tttttaaata atagttcaaa taaaagggct
      121 cacgatgaaa aaattaacag tggcaatttc tgctgtagct gcatcttaac gtataaacta
      181 gatttaataa taacgaacta gatttaataa caacgatatc cgtacctatc cggatatcta
      241 atgtcataag catcccttta attatcccct caacctcttg taactttccc actcttgaac
      301 tccacatagt ccaaacgccg gatgcaaaaa aatcacacgt gcatttctga aattgtgatg
      361 gaggaagata gtatcaatta tatgtgctta taaacgtccc cgattagcgc agaagtcacc
      421 ggattgttca ggctccaata ggttgagtat gccgtattta ttttccaaaa taaaaatcga
      481 tgctgttgct ccttcataaa atacttttac tctctggacg cttttcttag ctctcttggt
      541 gttatcttaa aacgattatg gaacctttca gtaaaacgag aaggacactt gtaaccattt
      601 tccctggcaa tctcgctgat gggttttact gtcgtttgta tagcagacaa cgcattattt
      661 aacctcacgt cgtccaatat actttgaaaa cttactccct cgcttgccag acggcgatgc
      721 aatgtggaaa cagaaatgta gagatatctc gctaccttgt ttgctgtcca ttttgttcca
      781 ggttcggata gcattaggtt ataacaacga cttattaatg attgtttact atctgataaa
      841 agtaaacaat taacatgatg tgctcctaat gaaagtagaa cgcccattgc taagtgttcc
      901 tgaatttgcg ttgagaagcc tcgggaaata gatgttttta gttgctccca acaatatatt
      961 aactcggaat tctgaggtaa gcagaaacct gtcgtgttac gaatttgatc ggttatcgca
     1021 taaagatttt ggaacttttc aattagatca atgggataat aaagcatttc tgcaagataa
     1081 agacctgctt ctgggtaatt cgcaatataa aattcatatc cacagggaaa taatattatc
     1141 tgattattat caacagttag actatgcgtc tcccaattga taactttttt tccctgacgg
     1201 atacgacatg aagctggcat aagaggctta accctgtgaa tctcatgatg tttatgcatc
     1261 cgtatttctt caatctttag gttagtccta tcttttgcca gcatactctt atcctacttt
     1321 acctcataaa cgggtattat ctcagcggtt gcaattttat tggcattaag catataacca
     1381 actaacgctc cgctggaatt agaatccaca ggaatcttat cagtttttag agcccatatt
     1441 ttaaactggt aatgatgggg tttatctcct ttgggaggac atgctccacc aaatcccgca
     1501 tagccaaaat catttcggcc ttggacagca ccagtcggaa gttttgtacc atcacgtctt
     1561 cctgcatcag cgggcaaata tgttaccgtt gctggaatat tagcaacagt ccagtgccac
     1621 catccgctgc ctgtaggcgc atctggatca tacacagtta cggcaaagct tttggtgcct
     1681 tctggagcac cagaccaggt taatgagggc gatgtattac ccccctcaca cccaactcca
     1741 gaaaatacat gagacgttgt aagttgctct cctgttttta tttcattact agtgacctga
     1801 aatgctgcgg cctgcgcaga aaatgttatg aatgccaata cacttgaaac gataagtttt
     1861 ttcataaaaa cctctttgtt gtgacctatc gttattttat ttgatgtgca ttgatctcat
     1921 tatgcaaaaa ggcgcaatga tcgcgcaaat gcaatcataa ttataaagtt gccccgaaca
     1981 ttagccataa tatgcacaaa tcagattcct gttatctcaa ggatgattat tttttaatgc
     2041 ggtgcgcttc gtgacaacaa gttaatgact aacacaccgg cacaaatcaa catcatgcct
     2101 atgatggctg gcaggtccag tcgttggcca aaaaatcccc aggaaagcaa actaatcaga
     2161 acgataccga ctcctgacca gatagcataa gcaatccctg taggtatata agccagtgtc
     2221 tgagctaata accaaaatga tgcacaatag caaataattg tgccaacaga gggccataac
     2281 cgtgtaaacc cctctgaaaa cttcattaag gttgtaccaa tgacctctgc aagtattgcg
     2341 ccaccaagat aaatataagg gttcatagca tattctttcc tgttcatact ggagagaatt
     2401 gtactacagt ttgaacacaa ctcaccgttt tcatcctggt gtatgtgtat ccagggggct
     2461 ctggcgtggc aagagataaa ggcaggaaca ataatgatca gaaggaaccc caggctaaca
     2521 aaagagttca ctcttcctat gagcaaagcg aagacaaaac atgttatcgc gtttacggat
     2581 cgtggatggc agaaaacttt caggatcagg tatccatttt aaaccagaaa ttgagtgagt
     2641 ttgccccatc catgccccac gcggtgagat cggatgtaat taacaacagg ttacaaaacc
     2701 tacacctgca tggacatcac ttcctgataa gccgccacca gcttattacg cacctgaatc
     2761 cccatttgca tagaaactga ggctttttgc atatcggtca tcacatcgtt taacgccacg
     2821 ccgggttcac cgagagtgaa tttttctgcc tgcgtgcggg cggctgtttg tgtatcgctt
     2881 atgcgatcga gcgcggcgtg cagctgcccg gcaaaactaa tggtcggttg tggcagtgat
     2941 tcctgcgcac gcgcactcat cgccgtagcc tgtaactggc tgataacccc ttcaatcccc
     3001 tgtatcgctg acattttcat ctcccggata atttctggta gcaaagctta ccagtaagtc
     3061 aataagacaa aggcgctaaa tagcaacaaa aaaaacgggt ttattggcgg atagaaaaaa
     3121 acgaaagcac aaataatggc agcgtcaatt tttcgagttt gctgacccgg gagtgagtct
     3181 tgttccactt tgccaataac gccgtccata atcagccacg aggtgcgcga tgaatgcgac
     3241 tgcagcccag acaaaatctc ttgagtggct taatcgcctg cgtgcgaatc cgaaaattcc
     3301 attgattgtt gccggttccg cggcagtggc ggtcatggtc gcactgatcc tgtgggcgaa
     3361 agcccccgac taccgcacat tattcagcaa tctttccgat caggatggtg gcgcaattgt
     3421 cagccaactg acgcaaatga atattcctta ctgcttcagc gaagccagcg gcgctattga
     3481 agttccggca gataaagttc acgaactgcg tctgcgcctg gcacaacaag gtttgcccaa
     3541 aggcggcgcg gtcggtttcg aactgcttga tcaggaaaag tttggtatca gccagttcag
     3601 cgaacaggtg aattatcagc gggcgctgga aggcgagctt tctcgtacca tcgaaactat
     3661 cggcccggta aaaggggcgc gcgtacatct ggcaatgccg aaaccgtctt tattcgtccg
     3721 tgaacaaaaa tccccttctg catcggtgac ggtaaatctg ttacccggcc gcgcactcga
     3781 tgaagggcaa attagcgcca ttgtgcacct ggtttccagc gccgttgctg gtctgccgcc
     3841 gggaaacgtc acgctggtgg atcagggcgg acatctgtta acccagtcca ataccagcgg
     3901 gcgcgatctt aatgacgctc agttgaaata tgccagcgat gtcgaaggcc gtattcagcg
     3961 gcgtattgaa gcaatcctgt cgcctattgt tggtaacggt aatattcacg cccaggtcac
     4021 ggcgcaactg gacttcgcca gtaaagaaca aacggaagaa cagtatcgcc ctaacggtga
     4081 tgaatctcat gcggcgcttc gttcacgcca gcttaatgag agcgagcaaa gcggttccgg
     4141 ttatccgggc ggcgtaccgg gggcgttgtc gaatcaaccg gcacctgcga ataacgcgcc
     4201 aatcagcacg cctccggcaa atcaaaataa ccgccagcag caggcgagca ccaccagcaa
     4261 tagcgggccg cgtagcacac agcggaatga aaccagtaac tacgaagtcg atcgcaccat
     4321 tcgtcatacc aaaatgaacg tgggcgatgt gcaacgtctg tcagtcgcgg tagtggtgaa
     4381 ttacaaaacc ttgccagacg gtaaaccatt gcctctcagc aacgaacaga tgaagcaaat
     4441 tgaagatctg acccgcgaag cgatgggctt ttctgaaaaa cgcggtgact cgctcaatgt
     4501 cgttaactcg ccgttcaata gcagtgacga aagcggcgga gaactgccgt tctggcaaca
     4561 gcaagcgttt atcgatcagt tgctggctgc cggtcgctgg ttgctggtac tgctggtggc
     4621 gtggctgctg tggcggaaag cggtacgccc gcagttaaca cgtcgcgccg aggcgatgaa
     4681 agctgtacag caacaggcgc aggcccgtga ggaagtggaa gatgcggtgg aagtccgcct
     4741 gagcaaagac gaacaactcc aacaacgacg cgctaaccaa cgtctggggg cagaagtcat
     4801 gagccagcgt atccgtgaaa tgtctgataa cgatccgcgc gtggtggcgc tggtcattcg
     4861 ccagtggata aataacgatc atgagtaacc tgacaggcac cgataaaagc gtcatcctgc
     4921 tgatgaccat tggcgaagac cgggcggcag aggtgttcaa gcacctctcc cagcgcgaag
     4981 tgcaaaccct gagcgctgca atggcgaacg tcacgcagat ctccaacaag cagctaacct
     5041 atgtgctggc ggagtttgag caagaagctg aacagtttgc cgcactgaat atcaacgcca
     5101 acgattatct gcgttcggta ttggtcaaag ctctgggtga ggaacgtgcc gccagcctgc
     5161 tggaagatat tctcgaaact cgcgataccg ccagcggtat tgaaacgctc aactttatgg
     5221 agccgcagag tgccgccgat ctgattcgcg atgagcatcc gcaaattatc gccaccattc
     5281 tggtgcatct gaagcgcgcg caggccgccg atattctggc gttgttcgat gaacgtctgc
     5341 gccacgacgt gatgttgcgt atcgccacct ttggcggcgt gcagccagcc gcgctggcgg
     5401 aactgaccga agtactgaat ggcttgctcg acggtcagaa tctcaagcgc agcaaaatgg
     5461 gcggcgtgag aacggcagcc gaaattatca acctgatgaa aactcagcag gaagaagccg
     5521 ttattaccgc cgtgcgcgaa ttcgacggcg agctggcgca aaaaattatc gacgagatgt
     5581 tcctgttcga aaatctggtg gatgtcgacg atcgcagcat tcagcgtctg ttgcaggaag
     5641 tggattccga atcgctgttg atcgcgctga aaggggccga gcagccactg cgcgagaaat
     5701 tcctgcgcaa tatgtcgcag cgtgccgccg atattctgcg cgacgatctc gccaaccgtg
     5761 gtccggtgcg tctgtcgcag gtggaaaacg aacagaaagc gattctgctg attgtgcgcc
     5821 gccttgccga aactggcgag atggtaattg gcagcggcga ggatacctat gtctgataat
     5881 ctgccgtgga aaacctggac gccggacgat ctcgcgccac cacaggcaga gtttgtgccc
     5941 atggtcgagc cggaagaaac catcattgaa gaggccgaac ctagccttga gcagcaactg
     6001 gcgcaactgc aaatgcaggc ccatgagcaa ggttatcagg cggggattgc cgaaggtcgc
     6061 cagcaaggtc atgagcaggg ctatcaggaa ggactggccc aggggctgga gcaaggtctg
     6121 gcagaggcga agtctcaaca agcgccaatt catgcccgga tgcagcaact ggtcagcgaa
     6181 tttcaaacta cccttgatgc gcttgatagt gtgattgctt cgcgcctgat gcagatggcg
     6241 ctggaggcgg cacgtcaggt gatcggtcag acgccaacag tggataactc ggcactgatc
     6301 aaacagatcc aacagttgtt gcagcaagaa ccgttattca gcggtaaacc acagctgcgc
     6361 gtgcacccgg atgatctgca acgtgtggat gatatgctcg gcgcaacctt aagtttgcat
     6421 ggctggcgct tgcggggcga tcccacactc catcctggcg gctgtaaagt ctccgccgat
     6481 gaaggcgatc tcgacgccag tgtcgccact cgctggcaag aactctgccg tctggcagca
     6541 ccaggagtgg tgtaatgacc acgcgcctga ctcgctggct aaccacgctg gataactttg
     6601 aagccaaaat ggcgcagttg cctgcggtac gtcgctatgg gcgattaacc cgcgctaccg
     6661 ggctggtgct ggaagccacc ggattacaat tgccgctcgg cgcaacctgt gtcattgaac
     6721 gccagaacgg cagcgaaacg cacgaagtag aaagcgaagt cgttggcttt aacggtcaac
     6781 ggctgttttt aatgccgctg gaggaagtgg aaggtgtcct gcccggcgcg cgggtttatg
     6841 ccaaaaacat ttcggctgaa ggactgcaaa gcggcaagca gttgccgctc ggtccggcgt
     6901 tattaggtcg cgttctggac ggcagcggta agccgctcga tggcctgccc tcccccgata
     6961 cgacggaaac cggtgcgctg attaccccgc catttaaccc gttgcaacgt acaccgattg
     7021 aacatgtgct ggacaccggc gtgcgcccaa tcaatgccct gcttaccgtt gggcgtgggc
     7081 agcgtatggg gctgtttgcc gggtccggcg ttggtaaaag tgtgctgctg gggatgatgg
     7141 cacgttacac ccgcgccgat gtcattgtcg tgggtttgat tggtgaacgt gggcgcgaag
     7201 taaaagattt tattgagaat atcctcggtg ctgaagggcg tgcacgctcc gtggtgattg
     7261 ccgctccggc ggatgtctct ccgcttctgc gaatgcaagg tgccgcctat gccacgcgca
     7321 ttgccgaaga ttttcgcgat cgtggtcagc atgtgttgct gattatggac tccctcaccc
     7381 gctacgcgat ggcccagcgt gaaattgcgc tggcgattgg cgaaccaccc gccaccaaag
     7441 gttatccacc gtcggtgttt gccaaattac cggcactggt cgagcgtgcc ggaaatggca
     7501 ttagcggcgg cggctcgatt accgcgtttt ataccgtgct tactgaaggc gatgaccagc
     7561 aggaccccat tgcagactcc gcacgggcga tcctcgacgg tcacattgtg ctgtctcgcc
     7621 gactggcgga agccgggcac tatccggcta tcgatattga agcgtcgatc agccgcgcaa
     7681 tgacggcgtt gatcagtgag caacattacg cgcgagtgcg caccttcaaa cagctgttgt
     7741 cgagttttca gcgtaaccgc gatctggtta gcgtcggcgc gtatgccaaa ggcagcgatc
     7801 cgatgctcga taaagccatc gccctgtggc cgcagctgga gggctattta caacaaggca
     7861 tttttgaacg tgcggactgg gaagcgtctc tccaggggct ggagcgtatt ttcccgacag
     7921 tgtcataacc caggagataa cggtagatgg cagaacatgg tgcgctggcg accctgaaag
     7981 atctggcaga aaaagaggta gaggatgccg cgcgcctgct gggtgaaatg cgtcgcgggt
     8041 gtcagcaggc ggaagaacag ctcaaaatgc tgatcgatta tcagaatgaa tatcgcaata
     8101 acctcaacag cgatatgagt gccgggatga ccagcaaccg ctggatcaac tatcagcagt
     8161 ttatccagac gctggaaaaa gccattactc agcatcgcca gcaacttaat cagtggacgc
     8221 agaaagttga cattgccctg aacagctggc gagaaaagaa acaacgtttg caggcctggc
     8281 agacactgca ggaacggcaa tccacggcgg cactgcttgc agaaaaccgc ctcgatcaga
     8341 aaaagatgga tgagttcgcc cagcgcgccg ccatgaggaa acctgaatga ttcgcttagc
     8401 gcccttgatt accgccgacg ttgacaccac cacattgcct ggcggcaaag ccagcgatgc
     8461 tgcacaagat tttctcgcgt tgttgagcga agcattagca agcgagacaa ctaccgacaa
     8521 agcggccccc caggtgctgg tggcaacaga taagcccacg acaaaaggcg agctgctgat
     8581 cagcgatatt gtttccgacg cgcaacaagc ggatttactg atccctgtgg atgaaacact
     8641 gcctgtcatc aacgtcgaac aatccacatc gacaccgtta accaccgctc atacgatgac
     8701 gttggctgcg gtggctgaca aaaatacgac aaaagacgaa aaagcggatg atctgaatga
     8761 agacctcacc gcaagcctga gcgccctttt tgcgatgttg ccgggttttg acaatacgcc
     8821 caaagtgact gatgcgccgt caaccgtgtt accggcagag aaaccaacgc tcttcacaaa
     8881 actgacttct gcgcaactca caacagcaca gcctgatgat gcccccggca cgccagctca
     8941 gccattaaca ccgctggtag cagaagccca gagtaaagcg gaagtcatca gcacaccttc
     9001 accggtgacc gctgccgcca gcccgctaat cactccacac cagacacagc cactgctcac
     9061 cgtcgccgcg cctgtcttga gtgcaccgct gggttctcac gaatggcaac aatcattaag
     9121 ccagcatatt tcgctgttca cccgccaggg acaacaaagt gcagagttgc gtctgcaccc
     9181 gcaggattta ggtgaagtgc aaatctccct caaagtggat gataaccagg cgcaaatcca
     9241 gatggtttcg ccgcatcagc acgtacgcgc cgccctggaa gcagcgctgc cggtactgcg
     9301 tacgcagctg gccgagagtg gcattcagtt agggcaaagc aacatcagtg gcgaaagctt
     9361 tagtggtcag cagcaggccg cttcccaaca acagcaaagc caacgcacag taaaccatga
     9421 acctctggcg ggggaagaag acgatacgct tccggttccc gtctctttac aagggcgcgt
     9481 aacaggcaac agcggcgttg atattttcgc ctaacgtcag aggtagcacc gtaatccgcg
     9541 tcttttcccc gctttgttgc gctcaagacg caggataatt agccgataag cagtagcgac
     9601 acaggaagac cgcaacacat gactgattac gcgataagca agaaaagcaa gcgatcgctt
     9661 tggctcccga ttctggtatt cattaccctc gcggcctgtg ccagcgcagg ttacagctac
     9721 tggcattcgc atcaggttgc cgctgaagac aaagcgcagc atcgcgtcgt gccctcaccg
     9781 gtcttctatg cgctggatac ctttacggtc aatttgggcg atgcggatcg cgtgctttat
     9841 atcggtataa ccctgcgact caaagatgaa gctacccgct cgcggctgag tgagtatttg
     9901 ccggaagtcc gtagccgttt gctgttactg ttttcgcgtc aggatgctgc cgtactggcg
     9961 acagaagaag gcaagaaaaa cctgattgcc gagattaaaa ccacgctttc caccccgctt
    10021 gttgccgggc aaccgaaaca ggatgtcacc gacgtgatgt ataccgcttt tattctgcga
    10081 taacgacatg ggcgatagta ttctttctca agctgaaatt gatgcgctgt tgaatggtga
    10141 cagcgaagtc aaagacgaac cgacagccag tgttagcggc gaaagtgaca ttcgtccgta
    10201 cgatccgaat acccaacgac gggttgtgcg cgaacgtttg caggcgctgg aaatcattaa
    10261 tgagcgcttt gcccgccatt ttcgtatggg gctgttcaac ctgctgcgtc gtagcccgga
    10321 tataaccgtc ggggccatcc gcattcagcc gtaccatgaa tttgcccgca acctgccggt
    10381 gccgaccaac ctgaacctta tccatctgaa accgctgcgc ggcacagggc tggtggtgtt
    10441 ttcaccgagt ctggtgttta tcgccgtgga taacctgttt ggcggcaatg gacgcttccc
    10501 gaccaaagtg gaaggtcgcg agtttaccca taccgaacag cgcgtcatca accgcatgtt
    10561 aaagctggcg cttgaaggct atagcgacgc ctggaaggcg attaatccgc tggaagttga
    10621 gtacgtgcgt tcggaaatgc aggtgaaatt taccaatatc accacctcgc cgaacgacat
    10681 tgtggttaac acgccgttcc atgtggagat tggcaacctg accggcgaat ttaatatctg
    10741 tctgccattc agcatgatcg agccgctacg ggaattgttg gttaacccgc cgctggaaaa
    10801 ctcgcgtaat gaagatcaga actggcgcga taacctggtg cgccaggtgc agcattcaca
    10861 gctggagctg gtcgccaact ttgccgatat ctcgctacgc ctgtcgcaga ttttaaaact
    10921 gaaacccggc gacgtcctgc cgatagaaaa acccgatggc atcatcgccc atgttgacgg
    10981 cgtcccggtg ctgaccagtc agtacggcac tctcaacggt cagtatgcgt tacggataga
    11041 acatttgatt aacccgattt taaattctct gaacgaggaa cagcccaaat gagtgacatg
    11101 aataatccgg ccgatgacaa caacggcgca atggacgatc tgtgggctga agcgttgagc
    11161 gaacaaaaat caaccagcag caaaagcgct gccgacgcgg tgttccagca atttggcggt
    11221 ggtgatgtca gcggaacgtt gcaggatatc gacctgataa tggatattcc ggtcaagctg
    11281 accgtcgagc tgggccgtac gcggatgacc atcaaagagc tgttgcgtct gacgcaaggg
    11341 tccgtcgtgg cgctggacgg tctggcgggc gaaccactgg atattctgat caacggttat
    11401 ttaatctccc agggcgaagt ggtggtcgtt gccgataaat atggcgtgcg gatcaccgat
    11461 atcattactc cgtctgagcg aatgcgtcgc ctgagccgtt agtgatgaat aaccacgcta
    11521 ctgtgcaatc ttccacgccg gtttctgctg cgccactgct gcaggtcagc ggcgcactca
    11581 tcgccattat tgccctgatc ctcgctgctg cctggctggt aaaacggttg ggatttgccc
    11641 cgaaacgcac tggcgttaac ggtctgaaaa ttagcgccag tgcttcgctg ggcgcgcgtg
    11701 aacgggttgt ggtggtcgat gtggaagatg cacggctggt gctcggcgtt accgcagggc
    11761 aaatcaatct gctgcataaa cttccccctt cagcaccaac agaagagata ccgcagaccg
    11821 attttcagtc ggtcatgaaa aatttgctta agcgtagtgg gagatcctga tgcgtcgttt
    11881 gttgtctgtc gcacctgtcc ttctctggct ggttacgccc ctcgccttcg cgcaactgcc
    11941 gggtatcacc agccagccgc tgcctggcgg tggacaaagc tggtcgctcc cggtgcagac
    12001 gctggtgttc atcacctcgt tgacgtttat tccggcaatt ttactgatga tgaccagttt
    12061 cacccgcatc atcattgttt ttggtttatt gcgtaacgcg ctgggaacgc cctccgcgcc
    12121 acctaaccag gtattgctgg ggctggcact atttttgacc ttttttatta tgtcatcggt
    12181 gatcgacaaa atttatgtcg atgcgtacca gccattcagc gaagagaaaa tatcaatgca
    12241 ggaggcgctg gaaaaagggg cgcagccgct acgtgagttt atgctgcgtc agacccgtga
    12301 ggcagattta gggttgtttg ccagactagc gaataccggc ccgttgcagg gacctgaagc
    12361 cgtgccgatg cgcattttgc tcccggccta cgtgaccagc gaattgaaaa ccgcatttca
    12421 gataggcttc acgattttca ttcctttttt gattatcgac ctggtgattg ccagcgtgtt
    12481 gatggcattg gggatgatga tggtcccccc cgccaccatt gctctgccct ttaaactgat
    12541 gctgtttgta ctggtggatg gctggcaatt gctggtcggt tcgctggcgc agagctttta
    12601 cagctagaga ggcaaaatga cacctgaatc ggtcatgatg atggggactg aagcgatgaa
    12661 agtcgcgctg gcactggctg ccccgctgtt gctggtggcg ctggtcacgg gccttatcat
    12721 cagtattttg caggccgcca cgcagatcaa cgaaatgacg ctgtcgttta ttccaaaaat
    12781 catcgccgta tttattgcca ttattattgc cggaccgtgg atgctcaatc tgttgctgga
    12841 ttacgtccgc accttgttca ctaacctgcc gtatatcatc gggtaggccg tacaatgatg
    12901 caggtgacaa gcgatcaatg gctttcctgg ttaagcctgt acttctggcc gttactgcgc
    12961 gtgctggcgc tcatttctac cgcaccaatt ctgagcgaac gcagcgtgcc gaaacgggta
    13021 aaactggggc tggcaatgat gatcacgttc gtcattgccc catcattacc tgccaacgat
    13081 gttcctgttt tttcgttctt tgctctgtgg ctggccgtgc agcagatcct gatcggcatt
    13141 gcgcttggtt ttaccatgca atttgccttt gccgctgtgc gaaccgctgg cgaaattatc
    13201 ggtctgcaaa tggggctgtc atttgcgacg tttgtcgatc cggccagcca tctcaatatg
    13261 cccgttttag cgcgtatcat ggatatgctg gcgttactgc tgttcctgac atttaacggt
    13321 catttatggt tgatttcact gctggtcgat acctttcaca ccctgccaat tggtggcgaa
    13381 ccgttgaaca gcaatgcgtt tctggcactc accaaagcgg ggagtttgat tttccttaac
    13441 gggctgatgc tggcgttacc gctcattact ctgctgctga ccctgaatct ggcattaggt
    13501 ttactgaatc gtatggcccc gcaattatcc atttttgtta ttggatttcc attaactctg
    13561 actgtcggta tctctttaat ggcggcatta atgccgttaa ttgcaccttt ttgcgaacat
    13621 ttattcagtg agatgtttaa tttgctggct gatattatta gtgaattgcc attaatataa
    13681 ttccgtaacg tttatcatgt tatcctaagg attatccgaa aaataatacc tacgaacatc
    13741 ttccaggata ctcctgcggc gaaatatttg ttttaagctc actcacatat cgcaacattt
    13801 actttaagac aattccaggc aaattataca acactttacg ggatagtaag tccgcctgaa
    13861 aaatcgcgag agtggcgcat taggtgaccc atgttgttcc gtttagtcat gatgaaatat
    13921 tcaggtaagg ggaattatcg ttacgcattg agtgagggta tgccatgtca acgattatta
    13981 tggatttatg tagttacacc cgactaggtt taaccgggta tctgttgagt agaggggtta
    14041 aaaaaagaga aatcaacgac attgaaaccg ttgatgacct tgccatagct tgtgattcac
    14101 agcgcccttc agtggtgttt attaatgagg actgtttcat ccatgatgct tctaacagtc
    14161 agcacatcaa gcacatcatt aatcaacatc ccaatacgtt atttatcgtt tttatggcaa
    14221 ttgccaatgt tcattttgat gaatatctat tggtcagaaa aaaattattg ataagttcta
    14281 aatcgattaa accggaatct ctcgacgata tccttggcga tattctgaaa aaagagacaa
    14341 cgataacctc ttttttaaat atgccgacgt tatcattgag ccgaaccgaa tcgagtatgt
    14401 tgcgaatgtg gatggcaggt cagggaacca ttcaaatctc tgaccaaatg aatatcaaag
    14461 ccaagaccgt ttcatcgcat aaaggtaata ttaaacgtaa gatcaaaaca cataataaac
    14521 aggttatcta ccatgtcgtc cgactgacgg ataatgtgac taatggtatt tttgtcaaca
    14581 tgcgctaaca cattctgact ggtggtttcc caccagtcag gctgaataag attactctgc
    14641 tttctccaca aagataccgt cctgatgccc tgcttcatta aagaaccaga tgccgagcgg
    14701 gtagtcttcc agcgaaacca ggtacattgt gccttcacta aactcctcaa ctgccagtac
    14761 cacgccagga cgacgcggac cgccatccgt tttgactgtt acccgatcat tcaccttcat
    14821 cgttttcctc ctgtggcttt gtgccagtgt agaacaattt cgtttttctg gcagctccag
    14881 gcgcgcgcga gtgctgattt tctcgacggt ctatacttaa gagatgccag gcggacttaa
    14941 cgactggcgg caacaacaga gtaacggttg cgaggaaaga tgatgaaaac cgcaaaagag
    15001 tacagcgata ccgcaaaacg tgaggtcagc gtcgatgtcg atgccctgct ggcggcgatc
    15061 aatgaaatta gcgaaagcga agttcatcgc agccagaacg attctgaaca cgttagcgtc
    15121 gatggacgtg aatatcatac atggcgtgaa ttggcggatg ccttcgaact ggatattcat
    15181 gacttcagcg tctctgaagt gaatcgttga atgcacaata aaaaaatccc gaccctgagg
    15241 gggtcgggat gaaacttgct taagcaagaa gcacttaaaa aattcgttac accaggaaat
    15301 ctgatgtgtt catcacctta tccgcaattt ttttcgctga caagaaaata ttcgcgatat
    15361 gaataattaa cgctacttat gaatagaaat gtgacgtgcg tcaccccttg gtggctctgg
    15421 cgtcaacggg atcgctcccg aaaaaatcat ctttgattat ttgtgctgtt ttgagaatca
    15481 tcctggtaag gggagaactg cacgaccatg ttttcaattc aacaaccact actggttttt
    15541 agcgatcttg atggcaccct gctggacagt catagttatg actggcaacc gactgccctc
    15601 tggctcagcc gtttacgcga agcgaatgtt cccgtcattc tctgtagcag taaaacttca
    15661 gcggaaatgc tgtacttgca aaaaacgttg gggctacaag ggttaccact gattgccgaa
    15721 aatggcgcag tgatccagct tgctgaacaa tggcaggata tagacggttt tccacgcatc
    15781 atctcaggta ttagccatgg cgaaatcagc caggttttaa atacgctacg cgagaaagaa
    15841 cattttaaat tcacgacttt cgatgatgtc gacgatgcaa ccatcgccga atggacggga
    15901 ttaagccgta gccaggcggc gctgacgcag ctgcatgagg cgtcggtaac gctaatctgg
    15961 cgcgacagtg acgagcgtat ggcacaattt accgctcgtc tgaacgaact gggcttacag
    16021 tttatgcagg gtgcgcgctt ctggcacgtc ctggatgcct ctgccggaaa agatcaggcc
    16081 gccaactgga ttatcgcgac ctatcaacaa ttatcaggca aacgcccaac cacacttggc
    16141 ctgggcgatg ggccaaacga tgcgccctta ctggaggtaa tggattacgc ggtgattgtg
    16201 aaagggctaa accgtgaagg ggtgcatctg catgatgagg atccgacccg cgtctggcga
    16261 acgcagcgtg aaggaccgga aggctggcgt gaagggctgg accatttttt ctccgctcgt
    16321 taagcgttat cgctcgcgca tacccgatta cgcccggcct gtttagcgag ataaagccga
    16381 cggtcagcca gtgactggag ttgttcaaaa tcataatcac cggtttcctc gctgctactt
    16441 acccccaacg aggcactgat gcgtatcgtc gtactcttag caatcaacat ctctttttca
    16501 tttaacttca ggcgaatacg ttctgcgact tccgcagcct ccgtcagact cgcgcctggc
    16561 agaatcacac aaaactcctc accaccgacc cgcccggcaa cgtcctgcgc acgcaaggaa
    16621 ctgctaatta atccggcagc atgagaaaga acacggtcgc ccgcctgatg accaaagcgg
    16681 tcattaatcg ctttaaaatg gtcaaggtcg acctgaatga cagaaaaagg atgttggtgc
    16741 gtctgacaca atttcgcgag cggacgggct ttttcaaaca gtgcgccacg attatataaa
    16801 cgggttaagg tgtcgtgcca cgcctgccac tgcaacgagc tttgcagaac atacatgttg
    16861 ctaaccatcc ggcgaatcac ataccaggag atgagtaaca tggtggtaaa gagcgcccac
    16921 agcagggtta atgcaatgct gatactgccg aaatcgccgc gcacgccttc gcttagcgta
    16981 tggacgcgca ccagcacacc gtcgaaatgg tccagacgtt cccagctaac atagcgactg
    17041 tccatacgaa tgccgccccg cgtgtcatgt tccatcgcct gcgccagcaa ggccagttca
    17101 cgaggatcaa aaatattccc tgttggatgg tcaggattgg aagaggtcaa aaatctcagc
    17161 ttactgtcat agagctgata ctcaccatcg aggtttttat cgatggcgtt tcttaaaaat
    17221 tgctgcatgg tacgcacggg aatactcatc cccagcacgc cataccagta gttattactg
    17281 tctaccggaa cactgacggt aacctgcggt tcagtattgc tggcgtgttc cggttgcgaa
    17341 gtaaaccagc gtaccgcacg gtgacgattt tctcgttgcg aatggccgat aaaccagggt
    17401 tgggtgacat agccgtaata acgcgttggt acattgcgcg taaacaaggt cggctgcgtc
    17461 gaaacgtaaa atccggcacg cgagacatac atcgcctgtt caaccatcga cgaggtgttg
    17521 tgcgccagtc gcagcaagta accaacttcc agtgcagcgg taatttcatt gtcgaggctt
    17581 tcattttcgc gagacaggag attcccctcg ctgactaatg catccgatac gccattgact
    17641 gacagggtgc gtcgtcggtt gagttcaatt tgccaggcgt gctcgtcgcg atgctgttcg
    17701 aactcggtta cagcattacg cagtgaagtg aaatccagtg gcgctacgag agcttcgcgc
    17761 atgccattac gcaaaaagat cagtttgtcg acgttatact gcaaatgctt atcgagcgcg
    17821 ttggctacgt tttccagatg attacgctgg ctggagatat aggcatcttc cagcactacc
    17881 acttcacgcc aggtcagcaa ggtggaaaaa agcaatacca cgataaagca gagattaacg
    17941 acatgaccag gccccaggcg gcgtgcgagt tttttcaacc agctctggtt ttccattttt
    18001 gtctcgtgct gcaccctgac gactcccaac ctgctttttt atgattctgg cataacctgg
    18061 cgatagcgta ccgcaaaaca gcgataattt tataatggtg gcatgataag acgcgactat
    18121 cattcattgc aggcattggc acaccaggcc ggaagtgatc cggcctggtg ttaatgatac
    18181 cctttcaaac ttcttccgca gaacgactcc cctcccccgg aacaagctcg gtttcatgaa
    18241 aagcttcccg cttgaccccg taaccgtcat accaacgaca ctcaaccata ccgctggagt
    18301 atccagtgac aatcatccgc gggccgccct ctttaaccgt aacttcctca ctaaccataa
    18361 agctcatact cgcctccttt tttcgagtga aacttgttca ccttagttga agatggcgaa
    18421 ttttgcataa tagcctgtac gagtattaat gtgcctgacc gcgcattttc gctacggctt
    18481 tcactcccag caccacgatg ccgccgatga taaatccaag aatcagattt aaaacagtcg
    18541 gtaatatcat cgccaccact gcactttgct gcccggcgaa atgttcaatg gcgtgatgca
    18601 gcggcgcaat accatgtacc acaataccgc cgccgacgag gaacatcgcc agcgtgccga
    18661 caatcgataa cgccttcatc aaccagggcg caatgatcaa taacccttta cctaatgcct
    18721 gcatcagcgc gctggatttt tccgccagcc aataccccag gtcatcaatc ttaacgataa
    18781 cccctaccag accgtagacg cccacggtca ctaccagcgc gatgcctgaa agcaccagca
    18841 cctgattaag caacggcgct tcggccacaa tccccagcgt gatggcgacg atttcggcag
    18901 acaatataaa atcggtacga atcgcccctt ttattttgtc cttttcaaac ttcagcggat
    18961 cctgcgccgc cagcttctcc agacgctgct ggctctgcgc cggatcttct ttatgtttac
    19021 gcgcctccag catatgcagc actttctcta ctccttcaaa gcagagaaac gcgccaccaa
    19081 tcatcaacag aggcgtaatc gcccacggga taaacgcact gatgatcagc gccagcggca
    19141 ccagaatcac tttattaatc agcgatcctt tcgccacgcc ccagaccacg ggaagttccc
    19201 ggttggcccg cacgcctgaa acttgttgcg cattgagcga taagtcatcc cctaatacac
    19261 cggcggtttt cttcgccgcc agtttgccca tcacggagat atcgtccagt agtgtggcga
    19321 tatcatcgag caacgtcagt aaactactcc cggccaaaag cattccctct catttttatt
    19381 gttgaagggt gtaagtatga agcaaaaacg gcagctctga tacttgcttc acaggtcaac
    19441 atttttttta caatcaagtg caattatatc tctcgccagc agaatgattt tcacgtttac
    19501 tatgaaccac ctttttattt ccaccgtgag ggattatgcg tttccgccag ttgttaccgc
    19561 tttttggcgc gctgtttgcg ttgtatatca tttggggctc aacctatttt gtcattcgga
    19621 ttggcgtgga aagctggcct ccgttaatga tggcaggcgt tcgattcctg gcagccggta
    19681 ttttattgat ggcatttttg ctactgcgcg gacacaagct ccccccgcta cgtccgctgc
    19741 tcaatgccgc gctgattggc ctgttattgc tggctgtcgg taatggcatg gtgacggttg
    19801 ccgagcatca aaatgttcct tccggcatcg ccgccgttgt ggttgcaacc gtgcccctct
    19861 ttaccctggt gcttcagccg cctgtttggc attaaaacgc gcaaactgga atgggtgggt
    19921 atcgccattg ggcttgccgg aatcatcatg ctcaatagcg gtggaaattt aagcggcaat
    19981 ccgtggggcg cgattctgat tttaatcggc tcgattagct gggcgtttgg ctcggtttat
    20041 ggctcgcgca ttaccttacc tgtagggatg atggcgggtg cgattgagat gctggcggca
    20101 ggcgtggtgt taatgatcgc gtcgatgatt gcaggtgaaa aactgacggc gctcccttcc
    20161 ctttcaggct tccttgcggt cggctatctg gcgctgtttg gttcgattat cgccatcaac
    20221 gcttatatgt atttaatccg taatgtcagt ccggctctcg ccaccagcta cgcctacgtt
    20281 aacccggtgg tcgcggtctt gctgggtacg ggactgggcg gagagacgct gtcgaagatt
    20341 gaatggctgg cgctcggcgt aattgtcttc gcggtggtac tggtcacgtt gggaaaatat
    20401 ctcttcccgg caaaacccgt agttgcgcca gttattcagg acgcatcaag cgagtaaatg
    20461 aatcccctgc gtgtcgatct gcgcgctggc accttcgccg cagatccact cttccagacg
    20521 ctcggtaagc gcttcatccg tcagcttctc gcgcccacgt aatgcacact cccagacaat
    20581 cagtacgcgc cagccgagtt cctgcaaacg actgatatcg cggcgatcgc gttcaacatt
    20641 tttacctatc ttctccagcc agaactcggt tcgagtcgca ggcactttaa acagatagca
    20701 gtgatgatga tgccagaagc agccatgggt aaatatcacg cagcgatatt catcaacgac
    20761 aaaatccggg cttccgggca gactggcgtc ctgaacgcga aacgccaggc cctgcccggt
    20821 taacagactg gcgaggcgct tctcaatcgc cgtatcacgc gtggcaatcg cgcgcatatt
    20881 tttgctgcga gtggccttat cgtgaacgtc ggccatgttg tgcctcttgc tgacgcaacg
    20941 ccaccgcctg tttgattttt ggctcaagca gttttgccac cgcggcaaag accggcacga
    21001 ccaccgagtt accgaactgg cgataggcct gagtgtccga aaccggaatg cggaatttcg
    21061 cttctcccgg cgcttcaaaa cccattaagc gcgcgcattc ccgaggcgtt aaccgacgtg
    21121 gacgatgttg ctgattcagc ggatcgtcaa aatctttctc acccgtggcc atatcccagc
    21181 cgcgatcgat taaaatttcc gcgccatctt tgtaataacg cgcagacagc gtacgcgtga
    21241 cgctttgcgg attgttcgga taaaccattc cataaccgaa gccgttaccg cgcgcctgat
    21301 gttttttcgc atatcgatag aggtacttcc acagcaccgg cgtcaggata tatttcgcct
    21361 cgaccatcgg gtccaacagc tgcgccagcg tcactcgctg tgcagggaaa cattcgctga
    21421 tatcacgcag agtaaaatcg gctttcagat taagatcgcg acgaaaaccc accagcacga
    21481 tgcgttcacg gtgctgcggc agaaaatgtt taccatcgat gattttcgga tcgtccggcc
    21541 cgttatcttc tgcatcagcc acgtcatagc ccagttcgtc cagcgtctgc atgatgatgc
    21601 ggaacgtttt accctggtcg tgacttttca ggtttttgac gttttcgagc acaaacatcg
    21661 ccggacgacg cgcgtcgata atgcgtacca catcaaaaaa cagcgtgccc tgggtatcgc
    21721 aggcaaaacc gtgcgcccgc ccgagcgagt tctttttcga tacgccagcc agcgaaaatg
    21781 gctgacaagg gaaaccggcc agtaaaacat cgtgttcagg aatgtgttga cgaatatgtt
    21841 ccgccgccgc ctcatcactc acgccttctt tatggctgag ggtgatgtcg cggatatctt
    21901 cattaaaatg atgcgtcgcc ggatcgcaat aatggttggc tttataagtg cgtaccgcat
    21961 gtttgttcca ttcgctggta aacacgcatt gtccgccaat cgattcaaaa ccgcgacgga
    22021 tgccgccaat tccggcaaat agatcgataa agcgaaacgc ataatgcgga tgatgtgccg
    22081 atggtttggg taacaacgtt tgcagatggg cgaactcttt ctcacttaaa cggtgccatg
    22141 ccgagtcatt cgccagcgca cgttttaaaa ttgccgcgct ccagtgattc tcacccacac
    22201 cattaagctg cgccaccagc gttttaacat cataaatttg cagcagtttc tccagcattg
    22261 cctgtgcggc attcccggtg ctgtatgaat cggttactga tatattttcc tgcatagatt
    22321 taaccggcca tctgagatgg ccgacaggtt aacataatta cagccattta gggccacgcg
    22381 cttatcccgc gggtgaaaac gcatctatca ccttgtgatc gacaccttca ttctcccccg
    22441 ccagttcggc actgagcttc gccataaact cgactaaaaa atgcgcgttg tgctgcgcca
    22501 gctgcttgcc tcttgcggtt tgcatggttt gcggtagctt cagcaactta gtctggaaat
    22561 gatccagcgc gtagcgttta tcatcaagcg ggcgatgctg tgcgaacgga tcttcaccat
    22621 caaacagcgc cacgcccaat gcccctgaaa cggcaaacac gcgcgccagg ccaatcgccc
    22681 ccagggcttc cagccgatcc gcatcctgga cgattttagc ttccgttgtt aagggggcga
    22741 tttgcgcgct gaaactgtga gcggcaatgg catgacaaac ggcctcgatt ttctccgccg
    22801 gaaattgctc gaactcttca cggagcaggc gacgcgtctc ttctgccgcc aggattgaag
    22861 aacgctgcct ttgcggatga tttttcgcca ggctcacaat atcgtgaaaa taacaggcgg
    22921 ttaaaatcac cagcatatcg acatcgtcat ctgccgccag cttctgtgcc gcgtcctggt
    22981 gctgatgatg gtttttcaac cagttttcga actgtgcctg ccagtgttgt aagtccatac
    23041 gcgctccgtt gccaaagagt cgccacctta gcagttttta ttcatagcgt ctgctacgtg
    23101 cgggacgagg tcgggtgtac caggcaatgc tgcctaacaa tgccccgacc agagccataa
    23161 ataaaaatcc gaccagtgcc cctaaccact ttcccgcggt agagcctaaa tcgctttcaa
    23221 atcccgacac cggggcatca gggatccggc tcatcaccca gatgtaacgc gccattgccc
    23281 agacgaaata gccacaccat gtatagaaag cccacaaagc caatttgccg ccagggctgc
    23341 gagagagtct ctcttccatc tttgtcgaac cgtattccat tttgagataa cccggaaaat
    23401 tcagacttag tactattatg tgataaaagt cacatttttc cacattggat gagccagtca
    23461 agactcaatc attttttgaa caaatagata aattttgcgc aacggcaata attactggaa
    23521 aaacagcaat aaagatttat ttatatcctt ggtaatagtt ttattaattc acccaaacaa
    23581 tatattagca ctgaatacaa ttataccttt tttgatttct gtgtcatgat aaccccgtta
    23641 ttagcctttt atcgtcttgt ttatattttt tgggccggca tgatgccggc tttttttata
    23701 ccttcattaa tgtgcgcgtg atcacgcctg ccgtttggcg caacaatcat tgatactccc
    23761 tatctttcca ttcaactcat aacatattga tatacattta taattttcat ggatgtttat
    23821 atttagaatt cataattctg aattatatca aatagataag aaacattttg aaatctttta
    23881 aatacatttg ttacacgcaa tctttaaaat aaaatgaact tcatagaata gtatccaaat
    23941 gtgctttt
//
LOCUS       DAJVQG010000054        23624 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJVQG010000054 DAJVQG010000000
VERSION     DAJVQG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23624
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.054"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            103..1329
                     /gene="eutH"
                     /locus_tag="OQM83_001912"
     CDS             103..1329
                     /gene="eutH"
                     /locus_tag="OQM83_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HCS1520187.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            1326..2729
                     /gene="eutA"
                     /locus_tag="OQM83_001913"
     CDS             1326..2729
                     /gene="eutA"
                     /locus_tag="OQM83_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCS1520188.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            2741..4102
                     /gene="eutB"
                     /locus_tag="OQM83_001914"
                     /pseudo
     CDS             2741..4102
                     /gene="eutB"
                     /locus_tag="OQM83_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
     gene            4123..5010
                     /gene="eutC"
                     /locus_tag="OQM83_001915"
     CDS             4123..5010
                     /gene="eutC"
                     /locus_tag="OQM83_001915"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HCS1520189.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESPSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            5020..5679
                     /gene="eutL"
                     /locus_tag="OQM83_001916"
     CDS             5020..5679
                     /gene="eutL"
                     /locus_tag="OQM83_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCS1520190.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            5692..6192
                     /gene="eutK"
                     /locus_tag="OQM83_001917"
     CDS             5692..6192
                     /gene="eutK"
                     /locus_tag="OQM83_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCS1520191.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            6238..7290
                     /gene="eutR"
                     /locus_tag="OQM83_001918"
     CDS             6238..7290
                     /gene="eutR"
                     /locus_tag="OQM83_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCS1520192.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(7296..8195)
                     /gene="hemF"
                     /locus_tag="OQM83_001919"
     CDS             complement(7296..8195)
                     /gene="hemF"
                     /locus_tag="OQM83_001919"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCS1520193.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(8199..9068)
                     /gene="amiA"
                     /locus_tag="OQM83_001920"
     CDS             complement(8199..9068)
                     /gene="amiA"
                     /locus_tag="OQM83_001920"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCS1520194.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTVAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            9282..9707
                     /locus_tag="OQM83_001921"
     CDS             9282..9707
                     /locus_tag="OQM83_001921"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCS1520195.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            9694..10143
                     /locus_tag="OQM83_001922"
     CDS             9694..10143
                     /locus_tag="OQM83_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCS1520196.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            10204..10779
                     /locus_tag="OQM83_001923"
     CDS             10204..10779
                     /locus_tag="OQM83_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCS1520197.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            10875..11774
                     /gene="yfeX"
                     /locus_tag="OQM83_001924"
     CDS             10875..11774
                     /gene="yfeX"
                     /locus_tag="OQM83_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCS1520198.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(11832..13136)
                     /gene="pbp4b"
                     /locus_tag="OQM83_001925"
     CDS             complement(11832..13136)
                     /gene="pbp4b"
                     /locus_tag="OQM83_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HCS1520199.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(13141..13314)
                     /locus_tag="OQM83_001926"
     CDS             complement(13141..13314)
                     /locus_tag="OQM83_001926"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HCS1520200.1"
                     /translation="MGLNSAFGPSGLVALPLMTSAQGILPAMAVYAGGILVAWVCGFI
                     FTTLFGCRNVNLD"
     gene            complement(13327..14565)
                     /gene="murP"
                     /locus_tag="OQM83_001927"
                     /pseudo
     CDS             complement(13327..14565)
                     /gene="murP"
                     /locus_tag="OQM83_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040446.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            complement(14569..15465)
                     /gene="murQ"
                     /locus_tag="OQM83_001928"
     CDS             complement(14569..15465)
                     /gene="murQ"
                     /locus_tag="OQM83_001928"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCS1520201.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            15629..16486
                     /gene="murR"
                     /locus_tag="OQM83_001929"
     CDS             15629..16486
                     /gene="murR"
                     /locus_tag="OQM83_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCS1520202.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            16615..17406
                     /gene="ucpA"
                     /locus_tag="OQM83_001930"
     CDS             16615..17406
                     /gene="ucpA"
                     /locus_tag="OQM83_001930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCS1520203.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            17577..18593
                     /gene="cysP"
                     /locus_tag="OQM83_001931"
     CDS             17577..18593
                     /gene="cysP"
                     /locus_tag="OQM83_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCS1520204.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            18593..19426
                     /gene="cysT"
                     /locus_tag="OQM83_001932"
     CDS             18593..19426
                     /gene="cysT"
                     /locus_tag="OQM83_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCS1520205.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            19426..20301
                     /gene="cysW"
                     /locus_tag="OQM83_001933"
     CDS             19426..20301
                     /gene="cysW"
                     /locus_tag="OQM83_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCS1520206.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            20291..21388
                     /gene="cysA"
                     /locus_tag="OQM83_001934"
     CDS             20291..21388
                     /gene="cysA"
                     /locus_tag="OQM83_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCS1520207.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            21522..22433
                     /gene="cysM"
                     /locus_tag="OQM83_001935"
     CDS             21522..22433
                     /gene="cysM"
                     /locus_tag="OQM83_001935"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCS1520208.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(22436..22804)
                     /locus_tag="OQM83_001936"
     CDS             complement(22436..22804)
                     /locus_tag="OQM83_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCS1520209.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            22909..>23624
                     /gene="pdxK"
                     /locus_tag="OQM83_001937"
                     /gene_synonym="thiJ"
     CDS             22909..>23624
                     /gene="pdxK"
                     /locus_tag="OQM83_001937"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCS1520210.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQL"
BASE COUNT         5379 a         6071 c         6707 g         5467 t
ORIGIN      
        1 gcttgccgcg tctgatccgg ccttcgggat tgcacatgta gggcggataa ggcgtttacg
       61 ccgcatccgc caataaataa caactaacag ggagtaaagg cgatgggaat taacgaaatc
      121 atcatgtaca tcatgatgtt ctttatgctg atagctgccg tagacaggat cctgtcgcag
      181 ttcggcggtt ctgctcgttt cctcggtaag ttcggtaaaa gtatcgaagg atcaggcggt
      241 cagttcgaag aaggctttat ggcaatgggc gctctgggtc tggcgatggt cggtatgacc
      301 gcgctggcac cggtactggc tcacgtactc gggccggtaa ttatcccggt ttacgaaatg
      361 ctcggcgcaa acccgtcaat gttcgccgga acgctgctgg cgtgcgatat gggcggcttc
      421 ttcctcgcca aagagctggc gggcggcgac gtagcagcgt ggctatactc tgggttaatt
      481 cttgggtcga tgatggggcc aacgattgtg ttttccattc cggtggcgct cggcattatc
      541 gaaccttctg accgtcgtta tctggcgctc ggcgtgctgg cgggcattgt gaccattccg
      601 attggctgta ttgctggtgg tctggttgct atgtactccg gtgtgcagat caacggtcag
      661 ccggtggaat tcactttcgc cctgatcctg atgaacatga tcccggtgat cattgttgcg
      721 attctggtgg cgctggggct gaaattcatc ccggaaaaaa tgatcaacgg cttccagatc
      781 ttcgccaaat tcctcgttgc attgatcacc ctcggtcttg ctgccgcggt agttaagttt
      841 ctgcttggct gggaactgat ccccggtctg gaccctatct ttatggcccc tggcgataaa
      901 cccggtgaag tgatgcgcgc cattgaagtt atcggttcta tctcctgcgt tctgttaggg
      961 gcgtatccaa tggtgctgct gctgacccga tggtttgaaa aaccgctgat gagcgtcggt
     1021 aaagtgctga atatgaacaa catcgcggca gccggcatgg tggcaacgct tgccaacaac
     1081 atcccgatgt tcggcatgat gaagcagatg gatacccgcg gcaaagtcat caactgcgcc
     1141 ttcgccgttt ccgctgcttt cgccctgggc gaccacttag gcttcgccgc tgccaacatg
     1201 aacgccatga tcttcccgat gattgtcggc aagttgatcg gcggcgtaac ggcgattggc
     1261 gtggcgatga tgctggtgcc aaaagaagac gcgaccacgg ctaaaaccga agcggaggca
     1321 caatcgtgaa cactcgccag ctattgagcg tcggtatcga tatcggcacc accaccaccc
     1381 aggtgatttt ctcccacctg gagctggtta accgtgcggc ggtgtcgcag gtgccgcgct
     1441 acgaattcat taaacgcgaa attagctggc aaagcccggt gttctttacc cctgtcgata
     1501 aacagggtgg tttaaaagaa gcggaactga aaaccttaat cctcgagcaa tatcaggctg
     1561 cgggcattgc gccggaaagc gttgattctg gtgccatcat catcaccggt gaaagcgcga
     1621 aaacccgcaa tgctcgtccg gcggtgatgg tgctctccca gtcgctgggc gattttgtcg
     1681 ttgccagcgc cgggccgcac ctcgaatccg tcattgccgg tcacggtgcc ggggcacaaa
     1741 ccctttctga acaacggctg tgtcgggtac tgaatattga catcggcggt ggcactgcga
     1801 actacgccct gttcgatgcc ggaaaaatca gcggcactgc ctgcctcaac gtcggtggtc
     1861 gcctgctgga aaccgacagc caggggcgcg tggtttacgc tcataaaccg gggcagatga
     1921 ttgtggatga gtgcttcggc gcaggcaccg acgcccgttc gctgaccggc gcgcagctgg
     1981 tgcaggttac ccggcggatg gcagaactga ttgtcgaagt gattgacgga acgctctcgc
     2041 cgctcgcgca ggcattgatg caaaccggtt tgctgcccgc aggtgttacg cccgaaatca
     2101 ttacgctttc tggcggcgtg ggcgaatgtt atcgccacca gcccgccgac ccgttctgtt
     2161 ttgccgatat tggcccgctg ctggcaacgg cgctgcatga ccatccgcgc ctgcgtgaga
     2221 tgaacgtaca gtttccggcg caaaccgtgc gcgccacggt gattggcgcg ggcgcgcata
     2281 ccctttcgct ctctggcagc acaatctggc ttgagggcgt acaactgccg ctgcgcaatt
     2341 tgccggtggc gatcccgatt gatgaaacgg atctggtgag tgcctggcaa caggcgctgc
     2401 ttcagctgga tcttgatccc aaaactgacg cgtacgtgct ggcgcttccc gcctcgctgc
     2461 ctgtgcgtta cgccgcggta ctgacggtca tcaacgcgct ggtcgatttc gtcgcgcgtt
     2521 ttccaaatcc gcatcccctg ctggtggtgg ccgggcagga ctttggtaaa gctctgggca
     2581 tgttgttgcg cccacagcta caacaactcc cgttggcagt cattgacgaa gtgattgtcc
     2641 gcgcggggga ctatatcgac attggtacgc ctctttttgg cggatcggtt gtgccggtga
     2701 cggtgaaatc actcgcattt ccttcctgag ggaacgactt atgaaactaa agaccacatt
     2761 gttcggcaat gtatatcagt ttaaggatgt aaaagaggtg ctggctaaag ccaacgaact
     2821 gcgttcgggg gatgtgctgg cgggcgttgc agcggcaagc tcacaggagc gcgtggcggc
     2881 aaagcaggtg ttgtcggaaa tgaccgtagc ggacatccgc aataatccgg tgattgccta
     2941 tgaagatgac tgcgtgacgc ggctgattca ggacgatgtt aacgaaacgg cctacaacca
     3001 gattaaaaac aggagcatca gcgaactgcg tgagtatgtg ctgagcgatg aaaccagcgt
     3061 ggacgacatt gcctttaccc gcaaagggct gacctcggaa gtggtcgcgg cggtagcgaa
     3121 gatttgctcc aacgcggacc tgatctacgg cgcgaagaaa atgccggtaa tcaaaaaggc
     3181 caataccacc atcggtattc cgggcacctt cagcgcccgt ttgcagccaa atgacacccg
     3241 tgacgacgtg caaagtatcg ctgcacaaat ctacgaaggg ctttccttcg gggtgggcga
     3301 tgcggtgatc ggcgttaacc cggtaactga cgacgtggaa aacttaagcc gcgtgttgga
     3361 caccatttac ggcgtgatcg acaaattcaa catcccaact cagggctgcg tactggcgca
     3421 cgtcaccacc cagatcgaag cgattcgtcg cggcgcgcct ggcggactga ttttccagag
     3481 tatctgtggc agcgaaaaag ggctgaaaga gtttggcgtg gaactggcga tgctcgacga
     3541 agcgcgcgca gtgggtgctg agttcaaccg tatcgccggg gaaaactgcc tctacttcga
     3601 aaccgggcaa ggttcggcgc tgtctgctgg cgctaacttc ggcgcagact aggtgacgat
     3661 ggaagcacgt aactatgggc tggcgcgtca ttacgatccg tttatcgtca acaccgtggt
     3721 cggctttatt gggccggagt atctctacaa cgaccgccag attatccgtg ctggcttaga
     3781 agatcacttt atgggcaagc tgagcggcat ctctatgggc tgtgactgct gttacaccaa
     3841 ccacgctgat gctgaccaga acctcaacga aaacctgatg atcctgctcg ccaccgcagg
     3901 ctgcaactac atcatgggga tgccgctggg cgatgacatc atgctcaact atcagaccac
     3961 cgcattccac gacactgcca ctgtgcgtca gttactcaac ctgcgcccgt caccggagtt
     4021 tgaacgctgg ctggaaagca tgggcattat ggcaaacggt cgcctgacca aacgggcggg
     4081 cgatccgtca ctgttcttct gatgacgcgg agataacaca tcatggatca aaaacagatt
     4141 gaagaaattg tacgcagcgt gatggcgtca atgggacaag cggcccccgc gccgtcagaa
     4201 gcaaagtgcg ccaccactaa ctgtgcggca ccggtgacct cggaaagctg cgcgctggat
     4261 ttaggttctg cggaagcaaa agcgtggatt ggtgttgaaa acccgcatcg cgcagacgta
     4321 ttaacagaac tgcgccgcag caccgtggcc cgcgtttgta ccggtcgtac cggtccgcgt
     4381 ccacgtacgc aggcgctgct gcgtttcctg gccgatcact ctcgttcgaa agataccgta
     4441 ctgaaagaag tgccggaaga gtgggtgaaa gcgcagggct tgctggaagt acgctccgaa
     4501 attagcgaca aaaatctcta cctgactcgc ccggatatgg gccgccgcct gtgtgcagaa
     4561 gctgttgaag cgctgaaagc acagtgtgtt gctaatccgg acgtacaggt cgttatttct
     4621 gatggcctgt caactgatgc catcaccgtg aactacgaag agatcctgcc gccgctgatg
     4681 gcgggcctga aacaggccgg gctgaaagtc ggcacgccat tctttgttcg ttatggtcgc
     4741 gtgaagattg aagatcagat cggtgagatc ctcggcgcga aagtggtgat cctgctggtg
     4801 ggggaacgtc cggggttggg tcagtcagaa agcccctcct gttacgccgt ttactcgccg
     4861 cgtatggcga ccaccgttga ggccgatcgc acctgcatct ctaacattca ccagggcggc
     4921 acgccgccgg ttgaagccgc agccgtcatt gtggatttgg cgaaacgtat gctggagcag
     4981 aaagcatccg gcatcaacat gacccgataa ggaggcatca tgccagcttt agatttgatt
     5041 cgaccgtcgg taaccgctat gcgggtgatt gcctccgtta acgctgaatt tgcgcgtgaa
     5101 ctaaaattgc cgccgcatat tcgtagcctc gggctgattt ctgctgattc cgatgacgtc
     5161 acctacattg cggctgacga agcgaccaag caggcgatgg tcgaagtggt atatggccgc
     5221 tcgctgtatg cgggagcagc acacggaccg tcaccgactg ccggtgaagt gctgattatg
     5281 cttggtgggc caaacccggc ggaagtgcgt gctggtctgg atgcgatggt tgcgcatatt
     5341 gaaaatggcg ctgctttcca gtgggctaat gacgcagaaa acaccgcatt cctggcacat
     5401 gtagtttcgc gtaccggttc ttatctttca tcaaccgccg ggatcacgct tggcgatccg
     5461 atggcgtatc tggtggcacc gccgctggaa gcgacctacg gcattgatgc agcgttgaaa
     5521 tctgccgacg tacagctggt gacctatgtc ccgccaccgt ctgaaaccaa ctactcggca
     5581 gcatttttaa ccggtagcca ggccgcgtgt aaagcagcct gtaacgcctt taccgatgca
     5641 gtgctggaaa tcgcgcgtaa tccaatccag cgtgcgtaac ggaggttgcc gatgatcaat
     5701 gcactgggat tgctggaagt ggacggcatg gtcgccgcga tagatgcagc ggatgccatg
     5761 ctcaaagcag ctaacgttcg tctgctcagt cacgaagtgc ttgaccctgg tcgcttaacg
     5821 ctggtggtgg aaggcgatct ggcggcgtgt cgtgcggcgc tggacgcagg ttgtgctgcc
     5881 gcgatgcgta ccgggcgtgt catcagccgc aaggagatcg gtcggccaga cgatgacacc
     5941 cagtggctgg ttactggctt taaccgccag ccgaagcaac ccgtaaagga acccgacgcg
     6001 ccagttatcg tcgcggaatc tgctgacgag ttgttggcgc tgttaacatc agtacgtcag
     6061 ggaatgacgg caggagaagt ggctgcccac tttggctggc cgctggaaaa agccagaaat
     6121 gcgctcgaac agctcttttc tgccgggacg ttacgtaaac gcagtagtcg ttatcgtctc
     6181 aagccccatt aacctgtcgg aggtgccggg tgtcctacaa cacccggcat taacatcatg
     6241 aaaaagaccc gtacagccaa tttgcaccat ctttatcatg aacccttacc cgaaaacctg
     6301 aagctcacgc cgaaggtcga agtggataat gttcatcaac gacagacaac ggatgtctat
     6361 gaacatgctt tgacgattac cgcctggcag cagatttacg atcagctgca tccgggcaag
     6421 tttcatggtg aatttacgga aattctgctc gatgatattc aggtttttcg tgaatacacc
     6481 ggtctggcgc tgcgtcagtc gtgcctggtc tggccgaact cgttctggtt tggcattccg
     6541 gcgacgcgcg gtgagcaggg atttatcggt tcgcaatgtc tgggaagcgc agaaatagcg
     6601 acgcgccccg gtggaaccga gtttgaatta agtacgccgg acgattatac gatcctgggc
     6661 gtagtgctgt ctgaagatgt catcacccgt caggctaact ttttgcacaa tccggatcgg
     6721 gtattacata tgctgcgtag ccagtcggcg ctggaagtga aagagcagca taaagccgcg
     6781 ctgtggggct ttgtccaaca ggcgctggcg acgttttgcg agaacccgga aaatctccat
     6841 cagcctgcag tgcgaaaagt gctgggggat aatttgctaa tggcgatggg ggcgatgctg
     6901 gaagacgcgc aaccaatggt gacggcggaa agcatcagtc atcagagtta ccgtagatta
     6961 ctttcccgcg cccgtgaata tgtgctggaa aatatgtctg agctggtgac ggtgctggac
     7021 ttgtgtaatc aactgcatgt cagtcgccgc acgctacaaa acgcgtttca cgctatttta
     7081 ggcattggcc caaacgcgtg gctgaaacgc attcgcctga acgccgtacg ccgcgaactg
     7141 ataagcccgt ggtcgcaaag cacaacggta aaagacgccg ccatgcagtg gggattctgg
     7201 catctggggc aatttgccac ggattaccag cagctgtttg ccgagaagcc gtcgttgacg
     7261 ttgcatcagc ggatgcggga atgggggtga gggagttaca cccaatccct gaccttaata
     7321 aactcactta acgccgcttc tgggctgcca tcttttggct gataatcata ctcccagcgt
     7381 accagtggcg gcattgacat caggatcgac tcggtgcgcc cgccagtttg caggccaaac
     7441 agagtgccgc gatcccagac cagattgaac tcgacataac gaccgcgacg gtagagctga
     7501 aaattgcgct cgcgctcgcc gtaggccatc gctttacgtc gctctacaat tggtaaataa
     7561 gcgtcggtgt agcctttgcc taccgcctgc ataaaggcaa aacagtggtc gaaatctggc
     7621 gtgttcagat catcaaagaa cagcccgcca ataccgcgct gttcgttgcg atgtttgagg
     7681 tagaagtaat cgtcgcacca ctttttgtaa cggggataaa cgtcttcacc aaatggcagg
     7741 cacaggtcac gggcggtgcg gtgccagtga atggcgtctt cttcaaaacc atagaaaggg
     7801 gttaaatcga agccgccgcc aaaccaccag acgggctctg cacccggttt ttcggcaata
     7861 aaaaaacgca cattcgcgtg gctggtggga acatacgggt tatgcggatg cactaccagt
     7921 gaaacgccca tcgcctcgaa actgcgcccg gcaagttccg ggcgatgagc ggtggcggaa
     7981 gcaggcatcg cctcgccgtg gacgtgagaa aagttgacgc ctgcctgttc gaaaacacca
     8041 ccattacgca acacccgact acgcccgccg ccgccagctt cgcgctgcca actatcttcg
     8101 acaaattctg cgccatcgac ggcggtcagc tgctgacaaa tcgtatcctg aaggttgagc
     8161 agaaactgtt taacctggtg tgcgtcgggt ttcataactt atcgcttttt cgaatgtgct
     8221 ttctggttgt cgaaccagtg gaaataactg atcacgcctt cagcaatcgc tgtggcgatt
     8281 ttctgacgaa acgccaccgt gcctaacagc cgctcttctt ccgggttggt gataaacgag
     8341 gtttccacca gcaccgaagg aaccgacggc gatttcaaca ccacaaacgc ggcttgttcg
     8401 gtgttgcggc tgtgcagttt atgcaccggc ttaatctttt tcagaatatg cgagccaagc
     8461 gtcaggctgt ttttaatagt atcggtttgc accagatcaa acagcacttg ctgcaatagg
     8521 tgatccttgt cagtcgcctt tttaccggca acttcatcgg cgcggttttc gcgttcagac
     8581 aggtatttcg ccattgcgct acttgcccca cggttagaga gggcaaatac cgaagcaccg
     8641 gcagctttcg ggttggtaaa gccatcggca tgaattgaca taaacagatc tgcgccatgt
     8701 ttatgggcga tttcaacgcg atcgtaaagt gggataaacg tatcgccaga acgcgttaaa
     8761 cgcgcatcaa tcccatgatt acgcaaaatg gaacggacgt ttttagcaat cgccagcacc
     8821 acatgttttt ctttcgaacc gttgcgtccg atcgctccgg tatcaattcc gccgtggcct
     8881 ggatcgagaa caacgacacg tttgccgcca gattttttgg ctttcggctt gctgtgtccg
     8941 ttgctggttt ttaaaggttc gtctttggcg atggcttgcg acattcctga caacgtcagg
     9001 gcagccaaac cggctttcag cacctggcgg cgcgaagtga gtgtttttag tggtttaaaa
     9061 gtgctcatac ggcctgagtt gtaataataa agttccagat gttatatcgt atcgcgtaat
     9121 ccgttacgac ggtttgatgt tgagaattgt tttacttttc atttcaatag ttgacaactc
     9181 cccattatgc cactttttca accgattttc gtcagatatt ggctaattcg accgttcacg
     9241 ccataatcag ccttttaacc cgcaaaaagg taacgaatac catggagata cgcgtatttc
     9301 gccaggaaga tttcgaagag gtcatcaccc tttgggagcg ttgcgacttg ctgcgtccgt
     9361 ggaacgatcc ggaaatggac atcgagcgta agatgaacca tgacgtcagt ttgtttttgg
     9421 tcgctgaggt aaacggtgaa gtggtcggaa cggtgatggg cggttatgac gggcatcgcg
     9481 ggtctgctta ttatcttggc gtgcatccag agtttcgtgg gcgtgggatt gccaatgcgt
     9541 tgcttaatcg gctggagaaa aagctgattg ctcgtggctg cccgaaaatt cagatcaatg
     9601 tgccggaaga taacgacatg gtgctcggaa tgtatgaacg cctgggatat gaacacgccg
     9661 acgtgctgag tctgggtaag cgtttgattg aagatgaaga gtactgagtt ccatcctgtc
     9721 cattatgatg cgcacggacg cctgcgttta cccttgctct tctggcttgt gctattgctt
     9781 caggcgcgaa cctgggtgct gtttgtcatt gccggtgcgt cgcgcgagca gggtactgcg
     9841 ctgctaaatc tgttttatcc cgatcacgat aatttctggc tggggctgat tccaggcatt
     9901 ccggcggtgc tggcgttttt gttgagcggt cggcgggcta catttcctcg cacttggcat
     9961 gtgctctatt ttctgttgct gttggcgcag gtggttttac tttgctggca accgtggctg
    10021 tggctgaacg gtgaatccgt tagcggtatc ggtctggcgc tggtagtggc ggatattgtg
    10081 gcgttaatct ggctgctgac caatcgacgt ttacgcgctt gttttaatga agaaaaagaa
    10141 taacggcact ttttggtgaa tttgcactcc aagcaacgtt attgaataac caaaggaagt
    10201 gacatgaaat cgctgcgttt aatgttatgc gctatgccgt tgatgctgac cggctgttcc
    10261 acgatgtcgt cagttaactg gtcggccgct aacccgtgga actggtttgg atcgtccacc
    10321 aaagtgagcg agcagggcgt gggtgaatta acggcgtcca caccactgca agaacaagcc
    10381 attgctgatg cgcttgatgg cgattatcgc ctgcgcagcg gaatgaaaac cgcgaacggc
    10441 aacgtggtgc gcttttttga agtgatgaaa ggcgacaacg tggcgatggt gattaacggc
    10501 gatcaaggca cgatcagtcg cattgacgtg ctggatagcg atattcctgc tgacaccggt
    10561 gttaaaatcg gtacaccgtt tagcgacctt tacagcaaag catttggcaa ttgccaaaaa
    10621 gctgatggtg atgataatcg tgccgtcgaa tgtaaagccg aaggcagtca acatattagc
    10681 taccagttca gcggggaatg gcgtggtcct gaagggttaa tgccttcgga cgataccctg
    10741 aaaaactgga aagtcagtaa aattatctgg cggcgttaat ttgcgtctga acaaaccgcc
    10801 gccgcgaaaa aacacgtaaa atagcgctca acaatgccac ggattgcgtg gcattcttat
    10861 tttcaggagg aacaatgtct caggttcaga gtggcatttt gccagaacat tgccgcgcgg
    10921 cgatttggat cgaagccaac gtgaaagggg aagttgacgc cctgcgtgcg gccagtaaaa
    10981 catttgccga caaactggca acttttgaag cgaaattccc ggacgcgcat cttggtgcgg
    11041 tggttgcctt tggtaacaac acctggcgcg ctctgagcgg cggcgttggg gcagaagagc
    11101 tgaaagattt tccgggctac ggtaaaggcc ttgcgccgac cacccagttc gatgtgttga
    11161 tccacattct ttctctgcgt cacgacgtaa acttctctgt cgcccaggcg gcgatggaag
    11221 cctttggtga ctgcattgaa gtgaaagaag agatccacgg cttccgttgg gtggaagagc
    11281 gtgacctgag cggctttgtt gacggtacag aaaacccggc gggtgaagag acgcgtcgcg
    11341 aagtggcggt tatcaaagac ggcgtggatg ccggcggcag ctatgtgttt gtgcagcgtt
    11401 gggagcacaa cctgaagcag ctcaaccgga tgagcgttca cgatcaggag atgatgatcg
    11461 ggcgcaccaa agaggccaac gaagagattg acggcgacga acgtccggaa acctctcacc
    11521 tcacccgcgt tgatctgaaa gaagatggca aagggctgaa gattgttcgc cagagcctgc
    11581 cgtacggcac cgccagtggc actcacggtc tgtacttctg cgcctactgc gcgcgtctgc
    11641 ataacattga gcagcaactg ctgagcatgt ttggcgatac cgatggtaag cgtgatgcga
    11701 tgttgcgttt caccaaaccg gtaaccggcg gctattactt cgcgccgtcg ctggacaagt
    11761 taatggcgtt gtaagcgttt ctgattcgat gattaaaggc cagccagtaa caaaactgac
    11821 tggccttttt attacttttg cttcaacgcc gcatacacct gatcaactac ccaaccataa
    11881 gttgcaatcg gtaactgacc gctttcgaac atattgggat ttttttgtgg atcggcaact
    11941 ggcgaatggg gcttgttgct taacatcaca atcgccatat gattgaccgg atcgataacg
    12001 gttactgtcc cggtccagcc agtgtgaccg taggtttgcg ggcttgccag cgtgccaaac
    12061 gtcggcgtca tggtggcatt accattcacg cgccagccga ggccaaaagt ggcatcttcc
    12121 ttagagctgg tggtgaacat cttcaccgtt tccgcactga acagctgcac atcgccatag
    12181 cccccgccgt tcagcatcgt ttgcattaac accgcaatat cgccggtatt ggaaaacaaa
    12241 cctgcgtgcc cggaaacgcc gcccatcgaa taaaaggctt tttcatcgtg cacctgaccc
    12301 cagagagtgg aggtgcggat attcgggaaa tggatcacgc catcgcgggt attgccgttc
    12361 aattccgtgg cggcaatttg ttgcggttta aagcctttca gcagcgggtt aaataccgta
    12421 tgtgtcaggc cgagcgggcg ataaatcgac tcttcaacat agcggtcaag cggctgaccg
    12481 gtaaccgact cgacgataaa tccaagcagc atataatcga catcgctgta gatatgtttg
    12541 ctgccgggct gatattccag cggcgtgcgc ttgatcatct ccagcgtctg gcctttatcc
    12601 tgggaatata acgcgcccgc gaccgcttta ttcgggtatt gcggatctgc cgggaaaccg
    12661 ccgctgtgat gcagcaggtc agaaatccgc agggtgtttt tgcctttaat ggtgtcattt
    12721 ggactatcgg caaaccccgg aatatatttc gcaatccgat cgtcaggatg cagtttgcct
    12781 tcgctcatca gcttttgcag ggcgaagttc gtggcgtaca ttttggtgtt tgaggccagg
    12841 tcatacagcg tcccggtggt ggctttgaca ggctgttcca tcaacacgct gccatcgtac
    12901 tttttcgccg ctccccaggc tttgcgatac acaatctgat tatctttaat gatcagcagg
    12961 tttacgccgg gataaccgac atcaatttgc tggctaatcc agcgatccat ctggttaagc
    13021 cgttcgacgt taaaccctgc tttctctggc gagctttctg tcagaacagg gtattttgcg
    13081 gcgctgacgc tacaactaac agccagcaga gaaagataga gcattgtccg tttcatgata
    13141 tcagtccaga ttgacgttac ggcagccaaa gagcgtggtg aaaataaacc cgcaaaccca
    13201 tgccaccaga atcccgccag cataaaccgc catggccgga aggatgcctt gtgcggaagt
    13261 catcagcggc agcgctacca gaccagacgg cccaaaggcg ctgtttaagc ccatcggcag
    13321 accccatcag gctatcaggc cgataaacaa accgcccgcc gcgccgccta aacaggcggt
    13381 aacaaacggc ttcatgcggg gcagggtgac accgtaaatc agcggttcac caacgcccag
    13441 caggccggga ataatcgccc cgcgtacctg actgcgtagc gcactgtgcg gttgcgcccg
    13501 ccagtagagt gccagcgccg cgcccacctg gcccgcgcct gccattgaaa ggatgggaaa
    13561 taagctgttg aatccctagc tgtccattaa cgcgaggtaa acaggaataa agccctgatg
    13621 cacgccaaat accacggcga tcaggaacag cccggctaaa accgcacaac cgaacggatt
    13681 actgttcagg tgcataaaca gccatgacat accttcgaat aaccagccac ccagcggcat
    13741 aatgatcagg tacgcaagcg tagcggtgat cagcagggtg attaacgagg tcagcagcat
    13801 gtcgagatcg tccgacataa agcggcgcac catgccttca atgcgggcgc aggcccacgc
    13861 ggcaatcagc acgccgataa tattgccgcg cggatcgatg ggcagaccaa agaaatcgtg
    13921 aaaaccggcg tagtcaccgg tggttgcagc ggggttataa ccgagcaaaa agagtgcggc
    13981 aataattgcg ccattgacgc ccgtgccgcc aaatgcctgg gcggcgttat agcccaccag
    14041 aatcaccagg aaagtgaaca aacctttgct gaacaccttc ataaaattca gcgcgtcggg
    14101 tagcgtgccc tgagcatctg ccggaacgtg catcaccgtg gcaattaacg tcgctatccc
    14161 cagcagcaga ccggcggcaa taaaaccggg gatcagcggc gtgaagatgg tggcgaattt
    14221 ggcgagaaat tgttgcacgc cggaggtttg tttggctttt aactgacgtt tgttctgggc
    14281 ggcgatttct gcggcatcct gtaccggtgc ctcgcccagc agctcgctca tcgcttttgc
    14341 ggcacgatgc gctttaccag gcccaaaaac gacctgcacc tggtcgctgg tcaaaatgac
    14401 gcccttcacg ccttccagcg ttttgatatt gggatcaacc agtgaactgt catgtacacc
    14461 cagacgcagg cgcgtcatac agttaccaca actggcgata tttcccagtc cgccgacacg
    14521 ggtaagaatg gtgttcagaa gttcactgct gatctctttg gccatgggtt attccttgtc
    14581 taaaacctga cgaataaagc cgccgtgttg atccaggcgt tttttagctt ctgcggcatc
    14641 gagatttttc agcaccatca caatggccgt tttacagttg cgctcgcagg caattaacgc
    14701 cgcttccgct tgttcggcat tacatccggt ggcgttttta acgatattga cctggcgcac
    14761 atgcagtttt tcgttggtgg cgaccacatc gaccatcagg ttgccgaaca ctttgccgga
    14821 tttaatcatc agcccggtgg aaagcatatt gagcaccagt ttctgcgctg tgcctgcttt
    14881 cattcgcgaa gaaccagtga ccacttcggc acctacaatc ggtgtaatgg caaactcggc
    14941 ggtggtggaa acggcactcc ccggattaca ggaaattccc actgtgcggc aaccgagctg
    15001 gcgggcgtat tccagtccgg caatcacata cggcgtacga ccgctggcgg caataccgac
    15061 caccacatcc tgtgccgtta aatttatatt tttcagatcg ttaatgccgc cttctcggct
    15121 atcttccgtg ccttccaccg cgtgctgaat ggcatattcg ccgccagcaa tcaaaccaac
    15181 caccagaccc ggtttcacgc cgtaggtggg cggacattcg ctggcatcca gaatccccag
    15241 acgaccggat gttcccgcac cgagataaat cagacgaccg ccgccgctta cctgggcgtg
    15301 gataacatcg atcgccgcgg cgatatccgg caggacgcgc tcaacggcaa gcggtacggt
    15361 tttatcttca tcgttgataa tccggcacat ttccagcgtc gatacgcggt caatttcagc
    15421 cgaggcggtg ttcgagcctt cagtaatcat cttttcaagt tgcatcggtg gtgaccttac
    15481 tatttacgat caaataattg cagatgtatg gaatcatata tcccgaattg acagacttaa
    15541 aggaatcatt gagtctacac ttgcagagtg tgaagagcgt cataagaact taagcggaat
    15601 ttttgctgcc agcccgggag agtggaagat gttgtacctg acgaaaatac gcaatgcgga
    15661 aagtgaattt accgagaacg agcaaaaaat tgctgacttt ctgcgggcca acgtgagtga
    15721 gctgaaatcc gtctcttcgc gcaagatggc gaaacagctc ggcatcagtc agtcgagcat
    15781 tgtgaaattt gcccagaagc tcggcgcgca aggatttact gaattgcgta tggcgttaat
    15841 tggcgaatac agtgccagcc gggagaaaac gaatgccacg gcgctgcacc tgcacagttc
    15901 gattaccagt gatgattcgc tggaagtgat tgcccgcaaa ctgaatcgcg aaaaagagct
    15961 ggcgctggag caaacctgtg cgttgttcga ctacgctcgg ctgcaaaaaa tcattgaggt
    16021 catcagtaaa gcaccgttta tccagataac gggcctgggc gggtcggcgc tggtggggcg
    16081 cgatctgtcg ttcaaactga tgaaaattgg ctatcgcgtg gcctatgaag ccgacacaca
    16141 cgtacaagcc accgtttccc aggcgttgaa aaaaggcgat gtacaaatcg ctatctctta
    16201 cagcggcagt aaaaaagaga ttgtcttgtg cgcagaagcg gcacgaaagc agggggcaac
    16261 ggtgattgcc attacttctc tggctgactc tccgctgcgg cggctggcgc atttcacgct
    16321 cgataccgtt tccggtgaaa ccgaatggcg tagctcgtcg atgtctaccc gcacggcgca
    16381 aaattctgtt accgatttac tgtttgttgg tcttgtgcaa ctcaatgatg tggagtcatt
    16441 gaaaatgatt cagcgcagca gtgagctaac acaacgcctg aaatgatgca taaagcagcg
    16501 actggattga gattttcctg aattagtgag ctgatccgca gcaatatttt gtttatcctg
    16561 tattttcaga gggaatggag tgtaacgctc tgtattaaca aggagagcat taaaatgggt
    16621 aaactcacgg gcaagacagc actgattacg ggcgcattgc agggaattgg cgaaggaatt
    16681 gccagaactt ttgcgcgtca tggcgcaaac ctaatcttgc tggatatctc ccctgagatc
    16741 gaaaagctgg cggacgaact gtgtggtcgt ggtcatcgct gtacggcggt tgtcgccgat
    16801 gtgcgtgacc cggcgtcggt agccgcagct atcaaacgcg cgaaggaaaa agaagggcgc
    16861 attgatatcc tggtgaataa cgcaggcgtt tgtcgtctgg gcagtttcct cgatatgagc
    16921 gatgacgatc gcgatttcca tattgacatc aatattaaag gcgtatggaa cgtcacgaag
    16981 gcggtgctgc cggagatgat tgcccgcaaa gatggtcgca ttgtgatgat gtcttcagtc
    17041 actggtgata tggtggccga tcctggcgaa acggcgtacg ccttaacgaa agcggcgatt
    17101 gttggcctga caaaatcgct ggcggtggag tacgcgcagt ctggtattcg cgttaacgcc
    17161 atttgcccgg gatacgtgcg cacaccaatg gcggaaagca ttgcccgcca gtcgaacccg
    17221 gaagatccag agtcggtgct gactgaaatg gcgaaagcaa tcccgctgcg tcgcctcgcc
    17281 gatccgctgg aagtcggcga actggcggcc ttcctcgcat cggatgaatc cagctattta
    17341 accggtacac agaatgtgat tgatggcggc agcacactgc cggagacggt tagcgtcggt
    17401 atctgattca cctctgtttc ctccctgcat ttgtggggag gatttcgttt taaacccggt
    17461 tcttattccc ttttcaactt ccaaatcacc aaacggtata taaaaccgtt actcctttca
    17521 cgtccgttat aaatatgatg gctattagaa agtcattaaa tttataaggg tgcgcaatgg
    17581 ccgttaactt actgaaaaag aactcactcg cgctggtcgc ttctctgctg ctggcgggcc
    17641 atgtacaggc aacggaactg ctgaacagtt cttatgacgt ctcccgcgag ctgtttgccg
    17701 ccctgaaccc gccgtttgag caacaatgga ccaaagaaaa cggcggcgac aaactgacaa
    17761 taaaacaatc tcatgccggg tcatcaaaac aggcgctggc aattttgcag ggcttaaaag
    17821 cagacgttgt cacttataac caggtgactg acgtacaaat cctgcatgac aaaggcaagc
    17881 tgatcccggc ggactggcaa acgcgcctgc caaataatag ctcgccattc tactccacca
    17941 tgggcttcct ggtgcgtaag ggcaacccga agaatatcca cgactggaac gacctggtgc
    18001 gctccgatgt gaagctgatt ttcccgaacc cgaaaacgtc gggtaacgcg cgttacacct
    18061 atctggcggc atggggcgca gcggataaag ctgacggtgg cgataaagcc aaaaccgaac
    18121 agtttatgac ccagttcctg aaaaacgttg aagtgttcga tactggcggt cgtggcgcga
    18181 ccaccacttt tgccgagcgc ggcctgggcg atgtgctgat cagcttcgag tcggaagtga
    18241 acaacatccg taaacagtat gaagcgcagg gttttgaagt ggtgattccg aaaaccaaca
    18301 ttctggcgga attcccggtg gcgtgggttg ataaaaacgt gcaggccaac ggtacggaaa
    18361 aagccgccaa agcctacctt aactggctct acagcccgca ggcgcaaacc atcatcaccg
    18421 actattacta ccgcgtgaat aacccggaag tcatggataa actgaaagac aaattcccgc
    18481 agaccgagct gttccgcgtg gaagacaaat ttggctcctg gccggaagtg atgaaaaccc
    18541 acttcaccag cggcggcgag ttagacaagc tgttagcggc ggggcgtaat taatgtttgc
    18601 agtttcatca agacgcgtgc tgccgggctt taccttaagc ctcggcacca gtctgctgtt
    18661 tgtgtgcctg attttgctgc tgccgctctc cgcgctggtg atgcagctgg ctcagatgag
    18721 ctgggcgcag tactgggagg tgatcaccaa cccgcaggta gtagcggcct acaaagtaac
    18781 gctgctgtca gcgtttgtgg catcgatttt taacggcgtt tttggcctgc tgatggcgtg
    18841 gatcttaacc cgctatcgct ttccgggccg cacgctgctt gatgcgctga tggatttacc
    18901 ctttgcgctg ccaacggcag tcgccggttt aacgctagcc tcgctctttt ctgtaaacgg
    18961 tttttacggt gaatggctgg cgaagtttga tatcaaagtc acctatacct ggctggggat
    19021 tgcggtggcg atggccttta ccagcattcc gtttgtggta cgtaccgtgc agccggtgct
    19081 ggaagagtta ggcccggaat atgaagaagc ggcagaaacg cttggcgcaa cgcgctggca
    19141 gagtttctgc aaagtggtgc tgccggaact ttctccggcg ctggtggcgg gcgtggcgct
    19201 gtcgtttacc cgtagtcttg gtgaatttgg cgcggtgatt tttatcgccg gaaatatcgc
    19261 gtggaagacg gaagtgacgt cgctgatgat ttttgtgcgc ttacaggagt ttgattaccc
    19321 ggcagcgagc gcgattgctt cggtgatcct cgcggcatct ctgctgctgc tgttctcaat
    19381 taacactctg caaagtcgct ttggtcggcg tgtggtaggt cattaatggc ggaagttacc
    19441 caattgaagc gttatgacgc gcgcccgatt aactggggca aatggtttct gattggcatc
    19501 gggatgctgg tatcggcgtt catcctgctg gtgccgatga tttatatctt cgtacaggcg
    19561 ttcagcaaag ggctgatgcc ggttttacag aatctggccg atccggacat gctgcacgcc
    19621 atctggctga cggtgatgat cgcgctgatt gccgtaccgg taaacctggt gttcggcatt
    19681 ctgctggcct ggctggtgac gcgctttaac ttccctggac gccagttact gctgacgcta
    19741 ctggacattc cgtttgccgt atcgccggtg gttgccggtc tggtgtatct gctgttctac
    19801 ggctctaacg gcccgctggg cggctggctg gatgaacata atctgcaaat tatgttctcc
    19861 tggcccggaa tggtgctggt caccatcttt gtgacgtgtc cgtttgtggt gcgcgagctg
    19921 gtgccggtga tgttaagcca gggcagccag gaagacgaag cggcgatttt gcttggcgcg
    19981 tctggctggc agatgtttcg ccgcgtcaca ttgccgaaca ttcgctgggc gctgctttat
    20041 ggcgtggtgt tgaccaacgc ccgcgcaatt ggcgagtttg gcgcggtgtc ggtggtttcc
    20101 ggctcgattc gcggcgaaac cctgtcgctg ccgttacaga ttgaattgct ggagcaggac
    20161 tacaacaccg tcggctcctt taccgctgcg gcactgttaa cgctgatggc gattatcacc
    20221 ctgtttttaa aaagtatgtt gcagtggcgc ctggagaatc aggaaaaacg cgcacagcag
    20281 gaggaacatc atgagcattg agattgccaa tattaagaag tcgtttggtc gcacccaggt
    20341 gctgaacgat atctcactgg atattccttc aggtcagatg gtcgcgttgc tggggccgtc
    20401 cggttccggg aaaaccacgc tgctgcgcat tatcgccggg ctggagcatc aaaccagcgg
    20461 gcatattcgc ttccacggca ccgacgtcag ccgcctgcac gcgcgcgatc gcaaggtcgg
    20521 tttcgtgttc cagcattacg cgctgttccg ccatatgacg gtattcgaca atatcgcttt
    20581 tggcctgacg gtgctgccgc gtcgcgatcg cccgaatgcc gcagccatca aagcgaaagt
    20641 gacaaaattg ctggagatgg tgcagcttgc tcatctggcg gatcgttatc cggcacagct
    20701 ttccggcggc cagaaacagc gcgtggcgct ggcgcgcgcg ctggctgtag aaccgcaaat
    20761 tctgctgctt gatgaaccgt ttggcgcgct ggatgcgcag gtgcgtaaag agctgcgtcg
    20821 ctggctgcgt caactgcatg aagagctgaa attcaccagc gtgttcgtga cccacgacca
    20881 ggaagaagcg acggaagtag ctgatcgtgt agtggtgatg agccagggca atattgaaca
    20941 ggctgatgcg ccggagcagg tatggcgcga accagcgacc cgttttgtgc tcgaatttat
    21001 gggcgaagtg aaccgcctgc agggaaccat tcgcggcggg cagttccatg ttggcgcaca
    21061 tcgctggccg ctgggctaca cacctgctta tcaggggccg gtggatctct tcctgcgccc
    21121 gtgggaagtg gatatcagcc gccgtaccag tctcgattcg ccgctgccgg tacaggtact
    21181 ggaagccagc ccgaaaggtc actacaccca attagtggtg cagccgctgg ggtggtacaa
    21241 cgaaccgctg acggtcgtga tgcatggcga cgatgccccg cagcgtggcg agcgtttatt
    21301 cgttggtctg caacatgcgc ggctgtataa cggcgacgag cgtatcgaaa cccgcgatga
    21361 ggaacttgct ctcgcacaaa gcgcctgata ggttgagtga atgttaaacg cccggaggcg
    21421 cttcccgcga tccgggcttt ttaatggcaa ggtttgtaac ctgtagacct gataagacgc
    21481 gcaagcgtcg catcaggcaa caccacgtat ggatagagat cgtgagtaca ttagaacaaa
    21541 caataggcaa tacgcctctg gtgaagttgc agcgaatggg gccggataac ggcagtgaag
    21601 tgtggttaaa actggaaggc aataacccgg caggttcggt gaaagatcgt gcggcacttt
    21661 cgatgatcgt cgaggcggaa aagcgcgggg aaattaaacc gggtgatgtc ttaatcgaag
    21721 ccaccagtgg taacaccggc attgcgctgg caatgattgc cgcgctgaaa ggctatcgca
    21781 tgaaattgct gatgcccgac aacatgagcc aggaacgccg tgcggcgatg cgtgcttatg
    21841 gtgcggaact gattcttgtc accaaagagc agggcatgga aggtgcgcgc gatctggcgc
    21901 tggagatggc gaatcgtggc gaaggaaagc tgctcgatca gttcaataat cccgataacc
    21961 cttacgccca ttacaccact accgggccgg aaatctggca gcaaaccggc gggcgcatta
    22021 cccattttgt ctccagcatg ggaacaaccg gcactatcac cggcgtttca cgctttatgc
    22081 gcgaacaatc caaaccagta acgattgttg gcctgcagcc ggaagaaggc agcagtattc
    22141 ccggcattcg ccgctggcct gcggaatatc tgccggggat tttcaacgct tctctggtgg
    22201 atgaggtgct ggatattcat cagcgcgatg cagaaaacac catgcgcgaa ctggcagtgc
    22261 gggaaggaat attctgtggc gtcagctccg gcggcgcggt tgccggagca ctgcgggtgg
    22321 caaaagctaa ccctggtgcg gtggtggtag cgatcatctg cgatcgtggc gatcgctacc
    22381 tttccaccgg ggtgtttggg gaagagcatt ttagtcaggg ggcggggatt taagattatt
    22441 gcgcaggcac cgttttatcc gtctgcgcaa ttaaggcatg taaaaaaggc tgtgcgttct
    22501 catcgctctt accggggatc ttcacaatat acggcttccc ggtaatcgat gacgacgaag
    22561 caaccttatc aataaactgc tcggcagtgt caatgcggtt acgggtattg ccgagcttca
    22621 gacgcagatg agaaaccgct tcatcgcagg tatgttcatc accgttgcgc acaaatatca
    22681 aatccttttt ctgtgctaat ccctccagca tggcgttgat gcgggcttct tcatgaacag
    22741 ttaacttcgc gtaagcgggg agtgtcatta gtagtgtaat gaccagacaa atgattttct
    22801 tcattgaatt accttatcct tgttttgtga tagccgattt gactgccaga atcaggtaaa
    22861 gtttcggttg gaatacgatg aggatcatcg gcaaggagaa aaaattaaat gagtagtttg
    22921 ttgttgttta acgataagag tagggcgctg caggcggata tcgtcgccgt gcagtcgcag
    22981 gtagtgtacg gcagcgtagg caacagcatt gcggtgcctg ctataaaaca gaacggcttg
    23041 aatgtctttg ccgtgccgac ggtattgctg agcaatacgc cgcattatga cactttctac
    23101 ggtggtgcga ttccggacga atggtttagc ggctatttgc gtgcgcttca ggagcgtgat
    23161 gcgctgcgcc aacttcgtgc tgtaaccacg ggctatatgg gaacggcatc gcaaatcaaa
    23221 atccttgccg agtggctgac tgcgctacgc aaagaccatc ctgacctatt gatcatggtc
    23281 gatccggtga ttggcgatat tgatagcgga atttatgtca aacctgacct tcccgaggcg
    23341 tatcgacaat atttactgcc gctggcgcaa ggaattaccc ccaatatctt tgagctggaa
    23401 atcctgaccg gtaaaaattg ccgcgatctc gacagtgcca ttgctgccgc aaaaagtctg
    23461 ctttcagaca cattaaaatg ggtggtgatt accagcgcct ccggtaatga agaaaatcag
    23521 gagatgcagg tggtggtggt cagtgccgac agcgtgaatg tcatttccca ttcacgggta
    23581 aaaaccgacc tgaaagggac tggcgacctg ttttgtgctc agct
//
LOCUS       DAJVQG010000055        23380 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJVQG010000055 DAJVQG010000000
VERSION     DAJVQG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23380
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.055"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(271..888)
                     /gene="tdk"
                     /locus_tag="OQM83_001938"
     CDS             complement(271..888)
                     /gene="tdk"
                     /locus_tag="OQM83_001938"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCS1520211.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1493..1906
                     /gene="hns"
                     /locus_tag="OQM83_001939"
     CDS             1493..1906
                     /gene="hns"
                     /locus_tag="OQM83_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCS1520212.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2050..2958)
                     /gene="galU"
                     /locus_tag="OQM83_001940"
     CDS             complement(2050..2958)
                     /gene="galU"
                     /locus_tag="OQM83_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCS1520213.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3160..4173)
                     /gene="rssB"
                     /locus_tag="OQM83_001941"
     CDS             complement(3160..4173)
                     /gene="rssB"
                     /locus_tag="OQM83_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCS1520214.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4265..5170)
                     /gene="rssA"
                     /locus_tag="OQM83_001942"
     CDS             complement(4265..5170)
                     /gene="rssA"
                     /locus_tag="OQM83_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCS1520215.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5283..5741
                     /locus_tag="OQM83_001943"
     CDS             5283..5741
                     /locus_tag="OQM83_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCS1520216.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5791..6633
                     /gene="purU"
                     /locus_tag="OQM83_001944"
     CDS             5791..6633
                     /gene="purU"
                     /locus_tag="OQM83_001944"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCS1520217.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6793..6877
                     /locus_tag="OQM83_001945"
     tRNA            6793..6877
                     /locus_tag="OQM83_001945"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6827..6829,aa:Tyr,seq:gta)
     gene            6925..7056
                     /locus_tag="OQM83_001946"
     ncRNA           6925..7056
                     /ncRNA_class="other"
                     /locus_tag="OQM83_001946"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7240..7917)
                     /gene="narI"
                     /locus_tag="OQM83_001947"
     CDS             complement(7240..7917)
                     /gene="narI"
                     /locus_tag="OQM83_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCS1520218.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7917..8627)
                     /gene="narJ"
                     /locus_tag="OQM83_001948"
     CDS             complement(7917..8627)
                     /gene="narJ"
                     /locus_tag="OQM83_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCS1520219.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8624..10162)
                     /gene="narH"
                     /locus_tag="OQM83_001949"
     CDS             complement(8624..10162)
                     /gene="narH"
                     /locus_tag="OQM83_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCS1520220.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10159..13902)
                     /locus_tag="OQM83_001950"
     CDS             complement(10159..13902)
                     /locus_tag="OQM83_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCS1520221.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14295..15686)
                     /gene="narK"
                     /locus_tag="OQM83_001951"
     CDS             complement(14295..15686)
                     /gene="narK"
                     /locus_tag="OQM83_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCS1520222.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16025..17821
                     /gene="narX"
                     /locus_tag="OQM83_001952"
     CDS             16025..17821
                     /gene="narX"
                     /locus_tag="OQM83_001952"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCS1520223.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17814..18464
                     /gene="narL"
                     /locus_tag="OQM83_001953"
     CDS             17814..18464
                     /gene="narL"
                     /locus_tag="OQM83_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCS1520224.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18465..19859)
                     /gene="ychO"
                     /locus_tag="OQM83_001954"
     CDS             complement(18465..19859)
                     /gene="ychO"
                     /locus_tag="OQM83_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCS1520225.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20044..20397
                     /gene="ychN"
                     /locus_tag="OQM83_001955"
     CDS             20044..20397
                     /gene="ychN"
                     /locus_tag="OQM83_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCS1520226.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20441..21136)
                     /gene="chaC"
                     /locus_tag="OQM83_001956"
     CDS             complement(20441..21136)
                     /gene="chaC"
                     /locus_tag="OQM83_001956"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCS1520227.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21294..21524)
                     /gene="chaB"
                     /locus_tag="OQM83_001957"
     CDS             complement(21294..21524)
                     /gene="chaB"
                     /locus_tag="OQM83_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCS1520228.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21794..22894
                     /gene="chaA"
                     /locus_tag="OQM83_001958"
     CDS             21794..22894
                     /gene="chaA"
                     /locus_tag="OQM83_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCS1520229.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23297..>23380
                     /locus_tag="OQM83_001959"
     CDS             23297..>23380
                     /locus_tag="OQM83_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCS1520230.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIV"
BASE COUNT         5860 a         6118 c         5626 g         5776 t
ORIGIN      
        1 ccttctgggg ccgttttttt gtttacccaa agcaactttt ccataaaccg acagcattag
       61 ccttcatcat atttgcgacg atgtataacg cctaaacaca gggatattgt actttacagg
      121 tcacaagtca acgtcggtgc ttaagagccc tgtgaggcgt atagcggcgt taaaaaactg
      181 ccgagaaggg tatatagccc ggaagaagtg cgtaaaacga actgacagga taaaagtgcc
      241 ctgctcaccc tgtcagtaaa gaaattctta ttaatcgtgg cgatgccttt cctgaatagc
      301 cgttaatgag ccgacttgta acgcctcttt atagtgttta cggcatacag aaacgtatcg
      361 ttcattacca ccaattacca cctgctcacc ttcgttataa ggtctgcctg cttgatcaag
      421 acgcagcacc atgcttgctt tacggccaca aaaacagatg gtttttaatt caaccagttt
      481 gtcggaccat gccagtaagt attggctgcc aataaataat tcacctcgaa aatcggtacg
      541 taaaccataa caaagtacgg gtatatcgag ttgatcgaca acctccgata attcatatac
      601 ttgttgtctg gttaaaaact ggcattcatc aaccagtacg caatgaattg cctgctgttc
      661 atgttccgca cgaatctcat caaataatga tgaattttgg ttaaataatt ttgcaggcga
      721 tgacaaacct atacgcgaac tgactttccc ggcaccaaag cgatcatcaa tttctgccgt
      781 atatacgaca gtgcgcatgc cgcgttcctg gtaattgtat gaagattgca acaatgctgt
      841 agacttaccc gcattcattg cggaatagta gaaatatagc tgtgccatca gccacaggcc
      901 ctcaatgata tgaaataaag ttggctggag tttatcataa ttcgtcagtt tttcagctca
      961 acagtatgca cagaagtggg tatatgcgtt ctcccttacg aagccttgca taatccttct
     1021 gagctatcat tacaactgcc tcgcttgtta taagcgggta aatgactact ggtaactatt
     1081 cacaatcttt aacctgttgc gcaagtaata gccctctgtt gacctccagg agatagtgca
     1141 atactaagtc catgctctta ttgcgactgt tctacttttc atcattcgct taatagggaa
     1201 ttctcgtaaa cacaactaat acagaagact gaaaggtcgt cagcctacga taatctcccc
     1261 ataaaatgtg acatgaatca ggaagtttta acctcacgtg ctgcgaaatc atcggtgtaa
     1321 atagggctat atgccgcgtc ttttctggct aattttatga aaagatattt attggcggca
     1381 caaaataaag aacaattttg aattccttac attcctggct attgcacaac tgaatttaag
     1441 gctctattat tacctcaaca aaccacccca atataagttt gagattacta caatgagcga
     1501 agcacttaaa attctgaaca acatccgtac tcttcgtgcg caggcaagag aatgtacact
     1561 tgaaacgctg gaagaaatgc tggaaaaatt agaagttgtt gttaacgaac gtcgcgaaga
     1621 agaaagcgcg gctgctgctg aagttgaaga gcgcactcgt aagctgcagc aatatcgcga
     1681 aatgctgatc gctgacggta ttgacccgaa cgaactgctg aatagccttg ctgccgttaa
     1741 atctggcacc aaagctaaac gtgctcagcg tccggcaaaa tatagctacg ttgacgaaaa
     1801 cggcgaaact aaaacctgga ctggccaagg ccgtactcca gctgtaatca aaaaagcaat
     1861 ggatgagcaa ggtaaatccc tcgacgattt cctgatcaag caataatctt ttgtagattg
     1921 cacttgctta aaatcccgcc agcggcggga ttttttattg tccggtttaa gacaatttaa
     1981 taagaataag ctataaaaaa acggcgtcga ttgctcaacg ccgtttcgtg gataacaccg
     2041 atacgaatgt tacttcttaa tgcccatctc ttcttcaagc caggctttaa attccgtgcc
     2101 aagggtgtta tgacgaatac cgtattcaac gaaggcctgc atgtaaccta atttattacc
     2161 gcagtcatgg ctcttccctt tcatatgata ggcttccacc gtttcttttt cgatcagcat
     2221 atcaattgcg tcggtgagct gaatttcatc accagctccc ggaggggttt ttgccagcaa
     2281 cggccaaata tccgcgctaa gtacgtaacg acccacaata gcgagattag acggcgcaac
     2341 atccgctttc ggtttttcta ccacaccaac catcggtacg ctttcacccg gcgctaattc
     2401 aacgcctttg caatccacaa cgccatatgc ggtcacatca gcaaccggtt caaccatgat
     2461 ctggctatga cccgtttcat caaagcggcg gatcatctct gccaggttat cctgtgacaa
     2521 atcggattca tattcatcca gaataacatc aggcaaaata acagctaccg gttcatcacc
     2581 cactaccggg tgagcacaca ataccgcgtg tcccaggcct ttcgccagac cctgacgaac
     2641 ttgcataata gtcacgtgcg gtggacaaat agactgcact tcatcaagca gttgacgttt
     2701 tacacgtttt tccagcattg cttccagttc aaaactggta tcaaagtggt tttcaataga
     2761 gtttttagat gagtgtgtaa ccagcacaat ttcagtaatg ccagccgcaa tacattcatt
     2821 cacgacgtat tgaattaatg gcttatcgac aagtggcagc atctctttcg ggatggcttt
     2881 cgtcgccggc aacatcctgg ttcctaatcc cgcaacgggg ataacggctt ttttgacttt
     2941 cgtattaatg gcagccattt aaattctcct ggactgttcg tgttttgaac gtgttcatat
     3001 ttctgtatcg catcccagta tatcagcacc ccgaaggggc taagttctga aaaggaggcg
     3061 agttagcata attaagacaa ttacttatac gaatggcgac gatagtacca ccagccgaca
     3121 ttagcaggta atgcaaattt agcccgcgtt atcgtttgct cattctgcag acaacatcaa
     3181 gcgcagtcga ccaccggttc cccatatttg gcattgccag gcatcgcatc gctggctcaa
     3241 ttgattcaaa taagcgttac ctaaagtgcc taacggaaca ccattactga tttgcacctg
     3301 gtgttcgccg gtatttaacg tcgcattcag acccgcagaa accagaatga gatttttcag
     3361 ttcgcgatga taatagccaa ctaatagcgg aaactgcccc ggcagattgg cctgacggag
     3421 taaatggttt acctgcttca ataacgcgcc caactctggc aaccgttgat tttggtgtgc
     3481 aagctgttcc tgtaataatc cgttaaacaa tgcgcgtaat aacaaggcag caagtacgcc
     3541 attatgtcca gctcgggtga catcaaggca ataaaatgcc agatcgtttt ccgaaagtgc
     3601 ggcaatatca agcaccaggc cgggtttgtc cgcggcaacc aattgacgat aattaacccg
     3661 gcaatgggaa atcacctgct gaaccggcgg ttgtagttcc tgtaataatt tcgccgctgc
     3721 ggcagggtta tcaaccattg catcccagtc gcgaaaaagc ctttcctctt cctcaacgcg
     3781 cgaattaaac atgctgggat agagacaggc aaaaaccatc tcgcgcaagc gattcagatc
     3841 tttaactggt ttcagcaaaa catcttcaac gcccagacgt aacgctttgg caatatctgc
     3901 catattttca gtggcagata tcaccagaac tggggtctgg tcgcctctgt tacgtatatg
     3961 ctccagcagt ttaagcccgt tcattcgtgg catcgcgata tcacatatca tcaggtctgg
     4021 agtgaaacct cccagcaact caagggcatc caccccatca gccgccagta ccgttgtcgc
     4081 tcccaatgag gaaaaccatg aatccagaag cgagcgaaat acctgctcat cttcaacaat
     4141 gagaatctgt tttccgacca atggctgcgt catgttctct cccctgactg gctttactca
     4201 atagtggcat gctattgcca cctgcgcctg tcagaattaa cttaagtgta gataaaaatt
     4261 ctggtcaaat gttggtgcgt accaacggca aaagttcgtc cattttcctt tccactgcca
     4321 gctgtccggc cgcaatggca gcgtgcgcgc gatggaaatc aagcgtagat atttgcgggc
     4381 aaacaggttg aatcagaata tcgggcggat cacctgccat gcggttcctt ttaaggcggt
     4441 tctccaacac ctggatagac gtggtcataa tctctgttgc cgttggcgct gtcacagccc
     4501 gacgtgtcgt tatgctgcct aacctttctt tcagacgcgc atgccacggt agagaatcac
     4561 cattctcgct attttcttca ctgacattaa aggagagcag atcttgttgc atcaaatgag
     4621 catcgtgctg caggtcgacc gctatcacaa tatcagcccc caatgcacgc gtaagagaaa
     4681 taggaattgg gttaactact gccccatcaa ccagccagta gccgttatgt gcaacgggtg
     4741 ccatgagtcc tggaatactg catgatgcgc gaatagcaag atggagatcg ccttcagtaa
     4801 accataattc acgccccgta cttaaattgg tggcaacagc cgcaaagcga cgggaacaat
     4861 tttcgatctc tgtttccggc attatttcgc gatattgatt gaagacacgc tcgccgcgca
     4921 gtaacccacc gcgctgccag gagaggtcca tcaggcgtaa aacgtcccaa taactgaaag
     4981 aggttaccca atcttccagc gcagataatc gatcgcatgc ataggccgcg cccaccagcg
     5041 aaccaattga acatcctgca acgatatcaa tttcaatacc cacttttttt agcgcattaa
     5101 taacgccaat atgcgaccaa cctctcgccg cgccagatcc cagcgccagc cctattttta
     5161 tctttctcat tattcccgca agattcccct ggaatgcaat cgtagccaca tcgctactgc
     5221 tcggttaaca tagtgctact caggcggtta atacgccgtt attgtttcca gggagattat
     5281 ttgtgtctca gctttgtccc tgtggtagtg ctgtcgagta tagcctatgt tgccaccctt
     5341 atgtgtctgg tgaaaaggtt gcacctgatc cagaacatct catgcgttcg cgttactgcg
     5401 cttttgtgat gcaagacgca gattatttaa taaagacctg gcatccctct tgtggagcag
     5461 cggcgttacg tgccgaattg atggccggat ttgcgcatac tgaatggttg ggattgaccg
     5521 tatttgaaca ttgttggcag gacgccgaca atatcggatt tgtcagtttt gttgctcgat
     5581 ttaccgaagg tggcaaaaca ggtgcaataa ttgaacgttc gcgatttttg aaagaaaacg
     5641 gtcaatggta ctatattgac ggtacacgtc cgcagtttgg tcgcaacgat ccctgccctt
     5701 gtggttcagg taaaaaattt aaaaagtgct gcggccaata atggttgacg gtacggttta
     5761 gcaaacactc tcaacaaggt ttttccagca atgcattcac tccaacgtaa agttctgcgt
     5821 actatttgtc cggaccaaaa aggtctgatc gcacgtatta ccaatatttg ctacaagcac
     5881 gagttaaata tcgtacagaa caatgaattt gttgatcacc gtaccgggcg cttttttatg
     5941 cgcacggaac tggaagggat ttttaatgat tccaccctgc tggcggatct cgatagcgca
     6001 ttgccagaag gctccgtgcg tgagctgaat cctgccggtc gtcgccggat agtgattctg
     6061 gtcactaaag aagcgcattg ccttggcgat ttgttgatga aagccaatta cggcggcctg
     6121 gatgtcgaaa tcgcggcagt tattggtaac cacgatactt tacgttctct ggttgagcgt
     6181 tttgatattc cgtttgagct ggtaagccat gaagggttaa gccgcaacga gcacgatcaa
     6241 aagatggcgg aagccattga tgcttatcaa cctgactatg tggtgctggc gaagtatatg
     6301 cgggtattaa cgccggaatt tgtggcacgc ttcccgaata aaatcatcaa tattcaccat
     6361 tcattcctgc ctgcgtttat cggcgcacgt ccttatcacc aggcctatga acgtggtgtg
     6421 aagattattg gcgcaaccgc tcactatgtg aatgacaatc tggacgaagg cccaatcatc
     6481 atgcaggacg ttattcatgt cgatcatacc tacacagctg aagatatgat gcgcgcaggt
     6541 cgtgacgtcg agaaaaacgt cttaagtcgt gcgctataca aagtactggc acagcgcgtc
     6601 tttgtttacg gtaatcggac gattattctt taatcgccag caaaaataac tggttacctt
     6661 taatccgtta cggatgaaaa ttacgcaacc agttcatttt tctcaacgta acactttaca
     6721 gcggcgcgtc atttgatatg atgcgccccg cttcccgata agggagcagg ccagtaaaaa
     6781 gcattacccc gtggtggggt tcccgagcgg ccaaagggag cagactgtaa atctgccgtc
     6841 atcgacttcg aaggttcgaa tccttccccc accaccatca cttttcaaaa gtccgtaaac
     6901 tctcaaacga atccgcaatt aaatattctg cccaagcggg gaaggatgag aagcttcgac
     6961 caaggttcga cttgagcgcc agcgagagag cgttgccgca ggcaacgacc cgaagggcga
     7021 agcgcgcagc gctgagtaat ccttccccca ccaccatcgc ttccttaaat aatcaaaacc
     7081 tgttactccg agctaatttt gcccgatggc accacgttta ccgggcctac aatatcctcc
     7141 cctgtaggtc ggataagcga agcgcatccg acaactcacc ccgcaccgtg cccataaaaa
     7201 aaccccgccg aagcagggtc tatgtgaact aaaattcgct tagtgacgag cgcgcaccag
     7261 ctggtactta cgtgtcagat actccaccgg tacgctccag atgtgtacca gacgcgagaa
     7321 cgggaacagc aggaataacg tcatccccag caccaggtgc agacggaaga taaacgccac
     7381 accatcgagg tgttgagaag cgccaccgtg gaaggtcacc accgactgcg cccagccaac
     7441 cagtttcatc atctcgctac cgtccatatg ctgagcggag aacggaatgg tcaacaggcc
     7501 cagcgcgcac tggataacga gcagcgacag gatcaggata tccgctccgg tagtggttgc
     7561 acgcacgcgt gggctgaaca gacgacgctt cagcaacagc acgccgccaa tcagacacag
     7621 cacgccgctg gcaccaccag caaacatcgc cattttctgt ttcacttcaa tcggcagcca
     7681 ggcttcatac atccagtgcg gcgtcagcat accgaagaag tgaccaacaa aaatccccag
     7741 aataccgata tggaacaggt tcgacgccag gttcatccct ttgcgatcca gcatctggct
     7801 ggacgccgcg cgccaggtgt actgcccgta gtcataacgc agccagctac caatcaggaa
     7861 gaccgccccg gctatgtacg ggtagatatc aaagaagaac atattcagga attgcattag
     7921 tgctgtcctc cggtggtgat attcagatat tgcggcgcga cggcaccggc aaagcgacgc
     7981 tgatgagcag tgattgctga atcaccgcag cctttgtcag caaagaattt aacctgctct
     8041 tcttcccaga cagcatccag cgcctgcggc gtatcatcgc gcgcttcgtc ggcaattttt
     8101 tccgccactt tgtcgctgtc gatagcggta ttggccaatt tcagcagcag atcaaacatc
     8161 acggcataac ggctttcacg ctgttgcaga cgcgcgctca gcaatgccag aatcggcgcg
     8221 atatctttca aaccttccac ggcttcgctt tgcggcagct gcgacaggta ctccagatac
     8281 agcggcagat ggtccggtaa ttcgcggctg tttaactgca agccgtgctg ctcgtactgc
     8341 gccagcaggt ccaccatcgc ctggccgcgg tcacgggatt caccgtgaac atgttcaaat
     8401 agcaacagtg acgtggcgcg accacggtcg aacagttcgc tgtactgggc ctgggcatcg
     8461 agcggatcca tcgccgttaa atcgcgcagg aaaatgccca gcgcatgggc atcttctttc
     8521 gacagatttt tcgacgcagc aatcgcctca aacatctctt gttgatgctg ccataaggca
     8581 gcatccggat attcaaggag acgcgataca atcacgagtt cgatcatgga tgcggctccg
     8641 ttttgctggt cacatcgatg gcatcgatac gacggctgtt gaacagattg aatttggtat
     8701 ctgaaccgtg gcagccatca ccaaaggtaa agccgcagcc atttttctcc gggaaggctt
     8761 cccgtgccag ttcacgatga ctactcggca ccacaaagcg atcttcgtag ttagcaatcg
     8821 ccagataacg gtacatctcc tgtgcctggg cttcggtcag accgacctct tccagcgcac
     8881 gggtatcaac tttaccgtca acggtttcag cacgtttgta atgacgcatc gccagcatac
     8941 gtttcagtgc gcgcagtacc ggtttggtat caccggcggt cagcagattc gccagatact
     9001 gtaccggaat acgcaagctt tcgacgtctg gcagaatgcc gttgctaccc agctcacccg
     9061 cgtctgctgc agactgaatc ggagacagag gcggcacgta ccagaccatc ggcagtgtgc
     9121 gatattctgg atgcagcggc agcgccagtt tccattccat tgccatttta taaaccggcg
     9181 actgctgtgc ggcttcaata acgctcagcg gaataccgtc tttaatcgcc tgctcgatga
     9241 ctttcggatc gttcggatcg aggaacacct ccagctgacg ctggtaaaga tctttctcgt
     9301 tctcggtgct ggctgcacgt tcaatcgcgt cggcatcgta cagcagcacg ccaagataac
     9361 ggatacgacc gacacaggtt tctgagcaca cggtcggctg acccgcttca atacgcggat
     9421 agcagaagat gcacttctca gacttaccgc tcttccagtt gaagtagatt tttttgtacg
     9481 ggcatccggt gatgcacata cgccagccac ggcatttatc ctggtcgatc aggacgatgc
     9541 catcttcttc acgcttgtaa atcgcaccgc tcgggcaggt cgccacacat gccgggttca
     9601 ggcagtgttc gcacaggcgc ggcaaataca tcataaaggt gttttcgaac tggctataca
     9661 tcgccttctg gatgttgtcg aagttcttgt ctttcgccag tttgtcaaac tcaccaccca
     9721 ggtcatcttc ccagttcggc cctttttcga ttttcgccat ccgttccccg gtaatcagcg
     9781 aacgcggacg ggcaatcggc tgcgatttgc tgccttccgg cgcggtatgc aggttctgat
     9841 agtcaaaatc gaacggctcg taataatcgt cgatccccgg cagatgcggg ttagcgaaga
     9901 ttttacccag caacatggca cggttaccca tgcgcggctg cagtttgccg ttgattttac
     9961 ggatccagcc gcctttgtat ttttcctggt tttcccagtc agtcgggaag ccctgacccg
    10021 gcttggtttc cacgttgttg aaccacgcgt attccacgcc ttcacggctg gtccagacgt
    10081 ttttacaggt aactgaacag gtgtggcagc cgatgcactt atcgagattc agcaccatgc
    10141 cgacttgtga acgaattttc attttacgct ctcctgtacc tggtcattgc cttcgccatc
    10201 taaccagtca atgttcttca tcttacgcac tacaacaaac tcatcgcggt tagaacctac
    10261 ggtgccgtag tagttaaagc cgtatgccag atgggcatag ccgccgatca tatgcgtcgg
    10321 tttcggcgtg atacgggtga ccgagttatg gataccacca cgctgttggg taatttccga
    10381 accaggcagg ttaacgatac gttcctgcgc gtggtacatc atggtcatcc ctgccggaac
    10441 acgctggctg acaaccgcac gggcagtcag agcaccgttg ctgttgaata cttcaatcca
    10501 gtcgttatcg gcgataccca gatctttggc atcggcttca ctcaaccaga ccaccggacc
    10561 accgcgacct aaagtcagca tcagcaggtt gtcgctgtag gtggagtgga taccccactt
    10621 ctggtgcggc gtcaggaagt tgagcgcttt ttccgggttg ccgttggatt tctggcctat
    10681 cacttctttc accgaacggg tgtcgatcgg cggacgataa accagcaggc tttcaccgaa
    10741 atcacgcatc cactggtgat cctgatacag ttgctgacga ccagagagcg tacgccatgg
    10801 gatcagctcg tgaacgttgg tgtaaccggc gttgtaagaa acgtgttcat cttccagacc
    10861 agaccaggtc gggctggaga taattttgcg cggctgtgcc tgaatatcga ggaagcggat
    10921 cttctcgtct tctttattca gcgccagatg cgtatggtca cgaccggtaa attcgctcag
    10981 ggcagcccag gctttcacgg ctacctgacc gttggtttcc ggtgccagtg tcaggatcat
    11041 ctctgccgca tcaattgcgg tgttcagcat cggctggcct ttcgccggac cttccgcttt
    11101 ggtgtagttg agcttacgca gcagatccat ctcgctctgg gtgttccagg caatcccttt
    11161 accgccatta ccgattttct ccatcaacgg gccaatagag gtaaagcgtt cgtaagtcgc
    11221 cggataatcg cgctctacga ccataatgtg tggcgcggtt ttacctggga tcaggtcgca
    11281 ctcgcctttt ttccagtctt tcacatccag cggctgcgcc agttcagcgg cagagtcatg
    11341 ctggataggc agcgtgacga tgtcggtttc tttacccaga tggccgacgc acacttcgga
    11401 gaatttcttc gcgatggctt tgtagatttc ccagtcgctt ttcgcttccc aggccggatc
    11461 gaccgccgca gacagcgggt gaataaacgg atgcatatcc gaagtattca tgtcgtcttt
    11521 ttcgtaccaa gtcgccgtcg gcaaaatgat gtcggaatag agacaggtgc tcgacagacg
    11581 gaagtccagc gtaaccacca gatccagctt gccttccaga ccattgtcct gccagtccac
    11641 ttcttccggc ttcacgccgc cctgttgccc cagatcttta ccctggatac cgtgctccgt
    11701 ccccagcagg tacttgagca taaactcatg acctttaccg gaagaaccga gcaggttaga
    11761 acgccagatg aacaggttgc gcgggtggtt tttaccgttt tctggttgtt ctgccgcaaa
    11821 acggatggaa ccctctttca gggatttcac cgtatagtcc accggattca tcccggcttt
    11881 ttccgcttcg ccagcgatag tcagcgggtt agtgcctaac tgcggtgcag acggcagcca
    11941 gcccatgcgt tccgcacgga cgttaaagtc gatcaagtgt ccggtatagc gggatttgtc
    12001 cgccatcggt gacagcaact cttccgccgt gacggtttca taacgccact ggctggagtg
    12061 gttatagaaa taagaagtgc tgttcatgtg acgcgccgga cgctgccagt caagggcaaa
    12121 cgccagcggc tgccagccgg tttgcggacg cagtttttcc tgacctacat agtgcgccca
    12181 gccgcccccg ctctgaccga cacagccgca gaaaatcagc atgttgatca gaccacgata
    12241 gttcatatcg aggtgatacc agtggttcag ccccgcaccg acgataatca tcgaacgacc
    12301 gtgcgtttta tcagcgttat cggcaaattc acgggcgatg cgaataatct ggctgcgaga
    12361 aacgccggta atctgctcgg cccaggccgg ggtataagct ttcacatcgt catagctggt
    12421 tgcacagtta acgtcgttca ggccacgttc cagaccgtag tttgccagcg tcagatcata
    12481 aacggtgctc accagggcgg tgctgccatc agccagttgc aggcgtttca ccggcagttt
    12541 gtgcagcagc acgttttcca gttccacttt gttgaagtgt tccgtgccgt cgccaccaaa
    12601 gtacgggaag cctacctcag cgatctcatc ctggctaccc agcaggctca gttgcagctc
    12661 ggtttcttcg ccagttttgc cgtcgcgctg ctcaagattc catttgccct tctcgcccca
    12721 gcggaagcca atagaaccgt tcggcgcaac catttcgcca ttggtattaa aggcgacagt
    12781 tttccattcc ggattgtttt cctggcccag cgcatcaacc agatcagcag cgcgcagcat
    12841 acgacctgca gcgtagtaac cgtcgcgttc ttccagcatc accagcatcg gcatgtcggt
    12901 gtagcgacgc acatagtcgg tgaaatactg gcttgggttg tcgaggtgga attcacgcag
    12961 cattacgtgg cccatcgcca gcgccattgc cgcatcggtg ccctgtttcg gtgccagcca
    13021 cagatcgcac agtttggcga tttcagcgta gtctggtgtg acggcaacag ttttggtgcc
    13081 tttgtaacgc acttcagtaa agaagtgagc atccggggta cgcgtctgcg gcacgtttga
    13141 accccaggcg atgatgtagc tggagttata ccagtcagca gattccggta cgtcagtttg
    13201 ctcgccccag gtttgcggag acgcaggagg caggtcgcag taccagtcgt agaagcttaa
    13261 gcaagtaccg ccaatcagcg agagatagcg tgcacccgat gcgtaagaaa ccatcgacat
    13321 tgccggaatt ggcgagaaac cagcaacacg gtccgggccg tagtttttga tggtgtaaac
    13381 gttagatgcg gcgatcagtt cgttcacctc ctgccaggaa gaacgaacaa atccaccgcg
    13441 tccacgcgcc tgcttaaagc ttttcgcttt atcggcgtct tcaatgatag aagcccatgc
    13501 ctcaaccgga tcgctatgca gcgccttcgc ttcacgccac attttcatca ggcgtttgcg
    13561 catcatcggg tatttcaggc ggttggcact gtaaagatac caggagtagc tggcaccgcg
    13621 cgggcagccg cgaggttcat ggtttggcag atccggacgg gtacgcggat agtcagtctg
    13681 ctgggtttcc caggtgacca gaccgttttt gacgtagatt ttccagctgc aggagccggt
    13741 gcagtttacc ccgtgggtag agcggacgat tttgtcatgc tgccaacgct ggcgatatcc
    13801 atcctcccag tcacggttgg tattgagaag ctggccatgc ccatcggcaa aggtttcacc
    13861 cttctgtttg aagtagcgaa accggtccag gaatttactc atcggttttc tcctgtggga
    13921 gcctgtcggc cctctgataa atcgacattg ctaatgttta agggcaaggt aacgctctga
    13981 aacagcgtgg gaattgataa cgatcaagag tgatggggat gaaaaataaa gtaaattcct
    14041 aagatacccc tttaggataa ttaacagcca ccacataaca gactgtaaat taagacgttt
    14101 tatacctgta gttatttaaa ctattaagat ttttatgcag atgggtatta aggagtattc
    14161 cccatgggta acatattaat ggctcttaat gaaatccggc gaaataagcc atataatatg
    14221 agtatgttgt aactaaatga cgcataaaaa aagcgcgaca tcatgccgcg caaaggataa
    14281 tcaaatatta agatttattt tttagaatgc cgaccatata ccgcccaggt aatcactacg
    14341 caggcgatat agaaaatcaa aaataccttc attgcgccga ctggcgaacc cgttaatgcc
    14401 agcgagctac caaacgcttt cgggataaag aagccaccaa tagcgccaat cgcagagatg
    14461 aaacccagcg ccgctgccgt gtcggttgcc gcttcacgca tcgcacgttc gtcagaaccc
    14521 ccttctgctt tcacgcgatc cattgtcagt ttacggaaga tcactgaaat catctggaaa
    14581 gtggaaccac tacccagccc agctgtcagg aacagcgcca ggaagaccgc gaagaacgcc
    14641 atgaagcttc cgccctgccc gtcagtcggt aaggtcagga acagcaggcc gctgaaaatc
    14701 gccatcagaa taaagttcac cagcgtgaca cgagttccgc ccagacggtc agataatgca
    14761 ccacctgcag aacgcgccag cgcaccaata aacggcccga agaaagcgta ttgcagaatc
    14821 tgaacatccg ggaactgcgt ttttgacagc atcgcaaagc ccgcggagaa gccgataaag
    14881 gagccgaagg ttgccagata cagcaggctc ataatccaca gatgaccccg tttgagtacc
    14941 ggcaactgct ccttgatgga ggctttcgag gtagcaagat cgttcatgcc aaaccacgcc
    15001 gcaatggtga agatggcaag gaacggcacc catatccagg acgcattcgc cagatacagc
    15061 tcagtcccat ccggctgttt gacgccctgg ctaccaaata ctgcgaaaat cgacagtgat
    15121 accaccagcg gagcaaccaa ctgcatgacg ctgacgccca tgtttcccag accaccattc
    15181 agacccagcg cgccaccctg cttctgtttc ggaaagaaga agctgatgtt tgccatactg
    15241 gatgcgaagt tcgcgccagc aaagccgcat agcagagaga tgatgatgaa gacgctataa
    15301 ggcgtggagg tatcctgcac ggcaaaaccc agccagacgc aaggaataat cagaataccg
    15361 gtgctgaacg ccgtccagcg acgaccaccg aagataggaa ccataaagga gtatggaaca
    15421 cgtaataacg cgccagaaac cgaaggcagc gcagtcaaca taaatagctg atcggtcgta
    15481 aaattaaaac cgactttcgg taagttcacc gcaacagcgc tgaacaacat ccatacgcaa
    15541 aacgccagca gcagacaggg aacggaaatc cacaggttac ggctggcaat acgttgaccg
    15601 cgttgttgcc agaacgcagg atcttccggt cgccaatctg taatgacagc tccagtagcc
    15661 ctttcggggg cggatgagtg actcatagac acctctgata ctcgtttcga tgtctgccac
    15721 cttagtgtct gtagctaaag gcaatttgat gtaaatcaaa cgataagata actttatcta
    15781 tcattgatat ttatcattac ccatagtgag tacagtgatt tcataaaaat tatgagattt
    15841 ttcacggtgc tgtaaaatcc ctacccttac cgatgtaaag cgactaacca cacggcaaat
    15901 aaggagtaac tctttccggg tatgggtata cttcagccaa tagccgagaa tactgccatt
    15961 ccagaatgta tcgtcacatt cattaaggtt attgctcatt taaagcctga aggaagaggt
    16021 ttgcatgctt aaacgttgtc tctctccgct caccctggtt aatcaggttg cgcttattgt
    16081 gttgctttct actgctattg gactggcagg gatggcggtt tctggctggc tggtgcaagg
    16141 cgttcagggc agcgcccatg cgatcaacaa agcgggatcg ctgcgcatgc aaagttaccg
    16201 tctgttggcg gcagtgccat taagtgagaa agacaagccc ttaattaaag agatggaaca
    16261 aacggcattt agcgccgagt taactcgagc agcagaacga gacgaacaac tggcgcaatt
    16321 acagggttta caagattact ggcgtaatga actgatccct gcactgatgc gtgcacaaaa
    16381 ccgcgaaacg gtgtcagcgg atgtcagcca gtttgttgcc gggcttgatc agctggtatc
    16441 tggttttgac cgcaccacgg aaatgcgcat tgagacagtg gtactggtcc atcgggtaat
    16501 ggcggtattt atggcacttt tactggtgtt cactattatc tggttgcggg cgcgactgct
    16561 acaaccgtgg cggcaactgc tggcaatggc gagtgccgtc agtcatcgcg attttaccca
    16621 acgcgcgaac atcagcgggc gcaacgaaat ggcgatgctt ggaactgcat tgaacaatat
    16681 gtctgcagaa ctggccgaaa gttatgccgt acttgagcag cgggttcagg agaaaaccgc
    16741 cgggctggag cataaaaatc agatcctctc ttttttatgg caggctaacc gccgtttgca
    16801 ttcccgcgcc ccgctgtgtg aacgcctgtc acctgtactc aacggcttac agaatttaac
    16861 cctgctacgt gatatcgaat tgcgggtgta tgacactgat gatgaagaga atcatcagga
    16921 gtttacctgc cagccagata tgacttgtga tgataaaggc tgccagctct gcccgcgcgg
    16981 cgtattaccc gttggtgatc gcggcacgac cctgaagtgg cggctggctg actctcatac
    17041 gcagtacggt attttgctgg cgaccctgcc gcaggggcgt catcttagcc atgatcaaca
    17101 acaactgctg gataccctgg ttgaacaact caccgccacg ctggcgctgg atcgccatca
    17161 ggaacgtcag caacagttga tcgtgatgga agagcgtgcc accattgcgc gcgaactgca
    17221 tgattctatt gcccaatctc tctcttgcat gaagatgcag gtgagttgtt tacagatgca
    17281 gggcgatgcg ctgccagaaa gcagccgcga actgttaagt cagatccgta acgaactgaa
    17341 tgcatcctgg gcgcagttgc gtgaattgct caccacattc cgcttgcagc tcaccgagcc
    17401 tggattacgt ccggcgctgg aggcgagttg cgaagagtac agcgccaaat ttggcttccc
    17461 ggtgaagctg gattatcaat tgccgcctcg cctggtgcct tcgcatcagg caatccactt
    17521 gttgcaaatt gcccgtgagg cattaagtaa cgccctcaaa cattcgcaag cgagtgaagt
    17581 cgtggtgacg gtggcgcaaa acgataatca ggtcaaactg accgtccagg ataacggctg
    17641 cggcgtgcct gaaaatgcca tccgcagcaa tcactacggc atgataataa tgcgcgaccg
    17701 tgcgcaaagt ttacgaggcg attgccgcgt ccgccgtcgt gaatcaggtg gcaccgaagt
    17761 ggtggtcacc tttattcccg aaaaaacttt cacagacgtc caaggagata cccatgagta
    17821 atcaggaacc ggctactatc ctgctgattg acgatcaccc gatgctgcga actggcgtaa
    17881 aacagcttat cagtatggca ccagatatca ccgtggttgg cgaagcgagt aatggcgaac
    17941 agggtattga actggcggaa tctcttgatc ccgatctgat cctgttagat ctcaatatgc
    18001 ccggcatgaa cggtctggaa acgctggata aactgcgcga aaagtccctc tcagggcgca
    18061 ttgtggtatt cagcgtctct aaccatgaag aagatgtggt caccgcactg aaacgcggcg
    18121 cggatggtta tctgttaaaa gatatggaac cggaagatct gctgaaagca ttgcatcagg
    18181 cagctgctgg cgaaatggta ttaagcgaag cattaacgcc tgttctggcc gccagcttgc
    18241 gcgctaaccg tgccactact gagcgcgatg ttaaccagtt aaccccacgc gagcgcgata
    18301 ttctcaagct gattgcccag ggtttgccga acaagatgat tgcccgccgc ctggatatca
    18361 ccgaaagcac agtaaaagtg cacgtcaagc acatgctgaa gaaaatgaag ctcaagtctc
    18421 gcgtggaagc agcggtatgg gtgcatcagg agcgcatttt ctgattacgg ttcccagcgc
    18481 agttcgtcgt ttgacaatgc atcgaacggt tcgacaagcg ttagcgtgat ctcattggag
    18541 gagacacgct gcccctggtt atcttccacc accaccgaca atcgccagtg attgctcgcc
    18601 ccttccccgt tctgccagtc aggcatgatc agcgtccagc cctccgcgct gttggcttgt
    18661 gcgcccggcg tcaaacttaa tatctgcgta tcgccctgcc aaatcagttg ccgaataccg
    18721 taacgactgc ggatttgtaa tttcagcggc actgtttcgc caggttttag atcccacggc
    18781 ggtgtcgcca gaaacaccgt taacgttttt cgctgtcggt actcaagagt cggtagatta
    18841 tttcgctgcg gattatcata gcgactacca cgtaacgact gactttcggc aacctcgccc
    18901 gcagaaagtt gttttttgag cggcacacca aagcggtaat taagattcag tccgaggtta
    18961 ttttgatttt cgccactttc accctgttta tgctgggccg tcacagtgac taatggcaca
    19021 ggggtgtaat ttaatcccag actcaacgcg acgggattgt gataacccgt accagagtta
    19081 aacaaatcaa cacgctcacc aaaatactgt tctaccctga cactggtatt gaggtgttga
    19141 tagaacggca tgcgcatccg ggctgtcagg tcatacccgc gcgccatccg ttgttcctgc
    19201 gtggctgtct gttcatgcca tgcagcaaac ggctgataaa agtttgccga tagtcgcaaa
    19261 tattcgcccc acgcttcggc accaaagccc gctcgctgaa gattttcgtc cagcaagttg
    19321 tcataaaaag tgttataacc caccagccag ttgccgcgcg cccagcgttg cccaacgccc
    19381 acattgctca ccaacccatc atcctgctga gtaagaccaa gctggctcca ggtgagataa
    19441 cgatcattat cttgtaacgg cacaaaccag cttccacgac tgccggtgaa atgtccttcg
    19501 ttatccactt tgacgtcaac actggcattt ccccacggtg atagccagga ctctacgtgc
    19561 tgattaacct gttgactaag cgcgtcgcgg acttttccca atgcgaaagc ttttgcctgc
    19621 tcgccagtat ccagcccgtt atcattcata ctggtttcgc cgaaatcttt cacaatttca
    19681 gcaaagtgtt tttccccatc atggttttcg ggagccatgc ctaaatccgg cagaccatca
    19741 ttgttattat caaagggatt tgccgctttt tgctcgaagg tagattgtgc gttagctgta
    19801 ccgcctgccg ccagcaagag taaataaaac gggataatgc gaggaacgaa acggctcaaa
    19861 acgtcggtaa cttccggtaa taatgaaaaa ccatagcctt aaagaataac gattactggc
    19921 aacttttgtc gtattttctc gcgctttcag gaaaatcctt aaaaaggtga ccgattggtc
    19981 agacgctgca gaagcagtaa gatagaagat gagtgtctaa ttccctgaat acaggagtaa
    20041 ataatgcaaa aaatcgtgat cgttgccaat ggcgcacctt acgggagcga atccttgttt
    20101 aacagcttgc ggctggccat tgcgttacga gagcaggaga gcaatctgga tctgcgtctg
    20161 ttcctgatgt ctgatgcggt cacagccggg ttgcgcgggc aaaaaccagg ggaaggctac
    20221 aacattcagc aaatgctgga gatccttacc gctcagaatg taccggtgaa attgtgcaaa
    20281 acctgtaccg acgggcgcgg gattagcaca cttcctctta ttgatggggt ggaaatcggt
    20341 actcttgtgg aactggcgca atggacgctg tcagccgata aagtgctcac attttaatgt
    20401 ttgagggggc gtctgacgcc ccactatgag ggaagtttac tcaggcgaat cccggccgta
    20461 acacaccatc cggataattc tccgccagca gtttttttac cgataccagc aaatcattca
    20521 gcccatcgtc ctgcattccc agtttgataa gctcctgttc cagtgaaaac aggtattgtg
    20581 cgttggttcc cagcggaccg ctcgccgccg caatcaacgg cgcgatgacc tgagcgcgag
    20641 tgtcagattc atattctgga tgtcgcgggt ccataataaa cacaatggcg tttactgtgc
    20701 gtccatcatc aagatcaagc tgacaccagg ttggcagata acagccggta atcatctcgc
    20761 gcttccacaa cagggttagt tcctgctcca gcgtctcttc tggtagtcga taggcgacgc
    20821 ctgtggtgcg tccgccctct ttcagtgcaa gcatccgtcc cggctggtgc gcagtcccgc
    20881 gcccggcggt caggcgcagg caaaacgcgc gatgccatcc aaccagtgta ccggtgcata
    20941 attcggtaaa ctccagtgcc ggattccaca tcaacgatcc ataaccgaag atccacaccg
    21001 gtccctcatt aggtcgacaa gccagcgtcg ccgccagcga agccgcccgt tgttctgctg
    21061 accataagag tgattcttca atggcaccaa atgccgtttt acaatcggca ttcatcaaga
    21121 aatcacgcgt tatcacctta cacctccgcc actgcatact tccctgcgac aactttttgg
    21181 tgtcttccgg acatttttac cgctcatttt gaaaccacta acgggacaat atgcaatcca
    21241 cggaccactt gcaaggcaac accgcacgaa tagctttaac taagctaccg gttttacgat
    21301 tttttatgcc atttatcatc atcccctttg gcatattcat gcttcacagc agcccaggcc
    21361 actttatgcg cggtttcttc gcgactggcg tcatcacgcc gatcttcttt atctttatat
    21421 tgatcccatg cgctgttgaa cgcttcttta tagatatcct gggcatgaga cggtagaacg
    21481 tgctttacgc tttccggcag atcgcttttc gttttatacg gcatcgttga ctccttttac
    21541 aaagttgaac attaagtgtg gtcaacaata tctactcttg ccaaaacata agaattttca
    21601 tgtcagactg ttcttaagaa acgctaaaac aaacattgtc taaacgtaat acttattttt
    21661 atgccccaat gagtaaaaaa tcgacagcgg ggtaaaaatt cgtaaaatgt taccgatatc
    21721 cagacacttt ctgttatttt tcctacctat tttttacatt ttcgataaat tcatctgcaa
    21781 agggataacc ataatgtcaa atgctcaaga ggcggtaaaa acccgccaca aggagacttc
    21841 gcttattttc ccggttctgg cgctggtagt gctgttcctg tggggaagca gccagacact
    21901 accagtggtc attgccatca atcttcttgc gcttattggt attttaagta gcgcctttag
    21961 tgttgtccgt catgcggacg tattagccca tcgcctggga gaaccttacg gttcgcttat
    22021 tcttagcctt tcagtggtta ttcttgaagt cagtttgatt tcagctttaa tggcaaccgg
    22081 cgacgccgcg ccaacgctaa tgcgtgatac gctctattca atcattatga ttgttaccgg
    22141 tgggctggtt ggcttttcat tattgttggg cggtcgtaag tttgccaccc aatatatgaa
    22201 tctgtttggt atcaagcagt atttaattgc gctgttcccc ctggcgataa tcgtactggt
    22261 atttccaatg gctctgcctg cggcgaattt ttcaaccggt caggcgttac tggtagcatt
    22321 aatttctgcg gcaatgtatg gcgtattttt gctgatccag accaaaacgc atcaaagttt
    22381 gtttgtctac gagcacgaag atgacagtga tgacgacgac ccgcaccacg gtaaaccgtc
    22441 tgcccatagc agcgtgtggc atgctatctg gttgattatc catctgattg ccgttattgc
    22501 agtcaccaaa atgaatgcca gcccgctgga gacattgctc gacagcatga atgcccctgt
    22561 cgcctttact ggcttcctgg tggctctgtt gattctgtcg ccggaaggtt taggtgcgtt
    22621 aaaagcagtg ttgaacaacc aggtccagcg cgcaatgaat ctgttctttg gttcagtgtt
    22681 agcaaccatt tcgcttaccg tacctgtcgt cacgctaatt gcctttatga cgggtaacga
    22741 attacagttt gcacttggtg cgccagaaat ggtggtgatg gtggcctctt tagtgctgtg
    22801 ccatatctcc ttctccaccg gacgtactaa cgtgctcaat ggcgcagcgc atctggcact
    22861 gtttgccgcc tatttgatga cgatatttgc ctgacagtta tatctcctcc gatgacggaa
    22921 atcgggggag ataagataaa ttcctgctta ttgtatttct tcatactacc tggacagctc
    22981 gttaatcaga attgcgagcc tgtaccacaa cctgcacgaa accactgcaa cagctgttga
    23041 aaagccggaa tgtactcttt attgcaaaca tttcagggat gaagtgaaga tgaattgaga
    23101 cgtagtatcc acaccaagcc ggaattatcc ggtgaggcgc aatgttgcgg gggctttatc
    23161 cctggtggca ttggttgctg gaaagagaaa acccccgcac gttgagaggt aaaacctgac
    23221 aacaaaacgg gggctaatct tgaaaccaga cacttcaaga atagccgcct tgttgtcatt
    23281 acaacaaggc ggctatatga cgctcgcgca gtttgccatg actttctggc acgacctggc
    23341 agcaccgatc ctggcgggga ttattaccgc agcgattgtc
//
LOCUS       DAJVQG010000056        23256 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJVQG010000056 DAJVQG010000000
VERSION     DAJVQG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23256
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.056"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..455
                     /locus_tag="OQM83_001960"
     CDS             <1..455
                     /locus_tag="OQM83_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCS1520231.1"
                     /translation="VPALVITAFVARRLLRFRHMLACRRRGLIVLTDRYPQDQIPGAY
                     DGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDLEVACARKPDHKRES
                     LARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITDFMTARGYH"
     gene            complement(504..2003)
                     /gene="pitB"
                     /locus_tag="OQM83_001961"
     CDS             complement(504..2003)
                     /gene="pitB"
                     /locus_tag="OQM83_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCS1520232.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(2295..4154)
                     /gene="gss"
                     /locus_tag="OQM83_001962"
     CDS             complement(2295..4154)
                     /gene="gss"
                     /locus_tag="OQM83_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCS1520233.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            4359..5225
                     /gene="yghU"
                     /locus_tag="OQM83_001963"
     CDS             4359..5225
                     /gene="yghU"
                     /locus_tag="OQM83_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCS1520234.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(5348..5596)
                     /gene="hybG"
                     /locus_tag="OQM83_001964"
     CDS             complement(5348..5596)
                     /gene="hybG"
                     /locus_tag="OQM83_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCS1520235.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(5609..5950)
                     /gene="hypA"
                     /locus_tag="OQM83_001965"
     CDS             complement(5609..5950)
                     /gene="hypA"
                     /locus_tag="OQM83_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCS1520236.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(5943..6431)
                     /gene="hybE"
                     /locus_tag="OQM83_001966"
     CDS             complement(5943..6431)
                     /gene="hybE"
                     /locus_tag="OQM83_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCS1520237.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(6424..6918)
                     /locus_tag="OQM83_001967"
     CDS             complement(6424..6918)
                     /locus_tag="OQM83_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCS1520238.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(6918..8621)
                     /gene="hybC"
                     /locus_tag="OQM83_001968"
     CDS             complement(6918..8621)
                     /gene="hybC"
                     /locus_tag="OQM83_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCS1520239.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(8618..9796)
                     /gene="hybB"
                     /locus_tag="OQM83_001969"
     CDS             complement(8618..9796)
                     /gene="hybB"
                     /locus_tag="OQM83_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCS1520240.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(9786..10772)
                     /gene="hybA"
                     /locus_tag="OQM83_001970"
     CDS             complement(9786..10772)
                     /gene="hybA"
                     /locus_tag="OQM83_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCS1520241.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(10775..11893)
                     /gene="hybO"
                     /locus_tag="OQM83_001971"
     CDS             complement(10775..11893)
                     /gene="hybO"
                     /locus_tag="OQM83_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCS1520242.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(12082..12369)
                     /locus_tag="OQM83_001972"
     CDS             complement(12082..12369)
                     /locus_tag="OQM83_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCS1520243.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(12488..13375)
                     /locus_tag="OQM83_001973"
     CDS             complement(12488..13375)
                     /locus_tag="OQM83_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS1520244.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            13532..14572
                     /gene="gpr"
                     /locus_tag="OQM83_001974"
     CDS             13532..14572
                     /gene="gpr"
                     /locus_tag="OQM83_001974"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCS1520245.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRIGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(14612..15106)
                     /locus_tag="OQM83_001975"
     CDS             complement(14612..15106)
                     /locus_tag="OQM83_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCS1520246.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            15297..16181
                     /gene="yghA"
                     /locus_tag="OQM83_001976"
     CDS             15297..16181
                     /gene="yghA"
                     /locus_tag="OQM83_001976"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCS1520247.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(16341..16766)
                     /gene="exbD"
                     /locus_tag="OQM83_001977"
     CDS             complement(16341..16766)
                     /gene="exbD"
                     /locus_tag="OQM83_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCS1520248.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(16773..17507)
                     /gene="exbB"
                     /locus_tag="OQM83_001978"
     CDS             complement(16773..17507)
                     /gene="exbB"
                     /locus_tag="OQM83_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCS1520249.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            17759..18946
                     /gene="metC"
                     /locus_tag="OQM83_001979"
     CDS             17759..18946
                     /gene="metC"
                     /locus_tag="OQM83_001979"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCS1520250.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDCVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            19086..19745
                     /locus_tag="OQM83_001980"
     CDS             19086..19745
                     /locus_tag="OQM83_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1520251.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(19785..20684)
                     /gene="yqhC"
                     /locus_tag="OQM83_001981"
     CDS             complement(19785..20684)
                     /gene="yqhC"
                     /locus_tag="OQM83_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCS1520252.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            20878..22041
                     /gene="yqhD"
                     /locus_tag="OQM83_001982"
     CDS             20878..22041
                     /gene="yqhD"
                     /locus_tag="OQM83_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCS1520253.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANDVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            22146..22973
                     /gene="dkgA"
                     /locus_tag="OQM83_001983"
     CDS             22146..22973
                     /gene="dkgA"
                     /locus_tag="OQM83_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCS1520254.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
BASE COUNT         5787 a         6227 c         5793 g         5449 t
ORIGIN      
        1 cagttccggc gctggtaatt acagcgtttg tcgcccgtcg cttactgcgc tttcgtcata
       61 tgcttgcctg tcgtcgtcgc gggttaattg ttctaaccga ccgttatcct caggaccaaa
      121 ttcctggcgc ttacgatggt acggtgttcc cacctaacgt tgaaggtggt cgttttgtct
      181 catggctggc aagccaggaa cgtaaagcgt ttcactggat ggcgagccat aagcctgatc
      241 tggtcatcaa actcaatgtt gaccttgaag ttgcctgtgc acgtaaaccc gaccataaac
      301 gggaatcgct ggcgaggaag attgccataa cgccacagtt aacctttggt ggtgcacaac
      361 tggttgatat cgatgccaat cagccactgg aacaggtgtt ggttgatgca gaaaaagcga
      421 ttacggattt tatgaccgcg cgtggttatc actagtcaaa aatggaaatg cccgatcgcc
      481 aggaccgggc attttcagga aggttaaatc aactgcaatg ctatccagta cagcccacca
      541 gaaagaaaaa ttgccgccgg taaagtaaac acccacgcca tcaggatgct ggttaccgtt
      601 ttacgctgta acccaccgcc gtccaccacc atcgtccctg ctaccgcaga cgagaggacg
      661 tgtgttgtgg agacaggcat cccaatataa ctggcaaggc cgatagacac tgccgccgtc
      721 atttgtgccg ccatgccttg cgcatacgtc atgccgcgct taccaatctt ctcaccgatg
      781 gtcatcgcta cacgacgcca gccaatcatg gtgccaatgc cgagcgccag tgctaccgcc
      841 atgatgatcc acaccggagc gtactcaatg gtgcttaaca tatcgctgcg cagttttttc
      901 agcaggttct ggtcttcttt actgacgcct ggcagtttcg ccagcttcgc ggaggtatca
      961 gagatgcaca gcataatgcg gcgcagctgg ctgcgctgac tcacgcttaa cggctcgtaa
     1021 ctttccatat tgcctggcag catcgtttta acgcgcgcaa tcgcatcaaa ggtatttgcc
     1081 ggatgacagt gaaactctgt tacttgcgtg ccatcagtcg atgctgcagg caatggaggt
     1141 tccatcgcaa tcaacttctg cggcagttca ggatgctgtt gcaggtagtg ttcgaagttg
     1201 gtgacggcat cgcgggtacg ggtaatttca tagccggacg cgttcatatt gacgacgaag
     1261 ccagcagggg caatccccac cagtaccagc atcaccaggc cgatcccttt ttgtccgtcg
     1321 ttcgcgccgt gcgaaaacgc cacgcccgca gcggaaacaa tcagcgcaat acgcgtccag
     1381 aatggcggtt tacgtttgcc ttttttcttt ttgcgatctt ccggaatgcg gtgaatacgg
     1441 tcacgctttt tcgtcccgct ccagtagcgt cgcagcagga atatcaggcc tcccgcaatg
     1501 accaggccga cgatagggga aacaatcagc gaggagaaaa ttttggtcac ttcacgcagg
     1561 tttaacgcat ccatcaccga tgagccggtt aacagcgcgt tggttaaacc gatgccgata
     1621 atcgcaccaa tcaaggtgtg cgaactggag gccggtaaac cgaagaacca cgttcccagg
     1681 ttccagataa tcgccgccag cagcatggaa aagaccatcg ccaggccgtg ggttgacccc
     1741 atattcagca acaaatcggt tggcaacata tggacaatgg cataggcaac gctaagtccg
     1801 cccaataaca cgccaaaaaa gttaaaaaat gccgccatca ccacagcaag ttgtggttgc
     1861 atggcacgag tataaataac ggttgccacc gcattcgccg tgtcatgaaa accattgatt
     1921 gcttcgtaga acaacacaaa tgccagagca agcaataaca aaagccctgt gtatatatca
     1981 aggccaacaa ataaatttag catatattag attacgccat tttgaattta cgaacggacg
     2041 cattatcagt gactttaacg gcatgggcaa agtgaaatat cattttttaa cgataaaaag
     2101 agtgttattt cttgtgtatc agacaataag cattcattca aaacctttag aaataatcaa
     2161 aacacaggta ttccactggt taatatattc acatatgaaa taaataaata ttagaacata
     2221 taaatattga atattttgat taatccccgt actgattatt cttcataatc agtacggggt
     2281 tgctacaaca tgtattactt tttcaccaca attaacggtt caatatcact ctcttttttg
     2341 atgaccagtg attcatcacc gcgcaaacac gtcccaccgt agttgccgcc cacggtgaag
     2401 gtacatacct gaatgtattt accgtccact ttcggcaaac accacagttg ctgatagatg
     2461 tttttctgct cggcaaattt accgctggtt ttgtccagca cctcttcatg atggctgacc
     2521 aggtcgatat tgctaccaca gcgaccggca atcggtttca ctgcgtaacc tgttttcacc
     2581 aattcatcat taacagtgaa atcggtatcc agcaggtaac gatggtgcgg gaacagcgac
     2641 cagaggatcg gcagaatcgc tttgttgccg gggatcaccg tccacagcgg ctcaaagact
     2701 agcacttccg ggcgcagcaa tacgtcgata agacgcactt cgttttgcgg atgaccggta
     2761 cggattggca ccgcagcaaa ctcacggtcg ctaacttcac gaatctgatc aaacgcggtt
     2821 tcccacgccc aggttttcca cacgcagtta accagtcgcc cttccccatc aatcagttgc
     2881 ccggcagcat cccagcccag ttcatccaat ccacgcaaga tacgcgtttc aaagcccgcc
     2941 tggtgcagcg cctgctccat aaactgcgcg tgatagtttt cctcgatatc tttgtcctgc
     3001 atgatatgga caaacggacg tgcacgactg tgtttccagg caccagccaa ttcgttaatc
     3061 agcccttccg ccggattgaa gccgttgcct ttatagccct gctccgccca acgttcgagg
     3121 atcaagcccg cttcggtatg acaggaggcg gagtcggcgt tgtactcgta aaccttcagg
     3181 ccacgctcat ccatgcagaa atccatacga ccagtgatca tatggtgacg gcgacgctgc
     3241 caggagagac gcaaacgtgg ccagaggatt ttcgggatgt cgaacagcgc cagcaggttg
     3301 tcatctttca gcaccttgtc ggttgcgtga agatacatca ggtgcagctc gttggtggct
     3361 ttaattagct cctgctcggc actctcggta atggtgtagt aatgataagg atcctgattg
     3421 atcacctgac cgttggcctg cacataggcg ttttgcagcg gatctttttc atccagccat
     3481 ttaccgtcaa actggccttt gttttccagg cgcgctccgc tgattttcag cagctcgcct
     3541 gcaatttccg gctgcggtaa gctgtattca gtatcttccg tctggatcat ccagcccaga
     3601 atggtggtgt catcaaaagt gtctttcagg gtatagcagc cgttttcgac caccatctcc
     3661 agctcacgcg tccactgttg cccttgcggc aacggggaat gaatcacgtt ctgttccgca
     3721 atacggactt tgttgccatg caattgggta atgatggcga catggccagt gtctttaaat
     3781 tcaccgcctt tatcccagat aagaagcgca cccgcgaccg gcgcacgcgg cgagccgtta
     3841 ggaaatgcct gcaatggcag gatgttgtca ttaaccactt ctcgcaggaa gcgcagcgag
     3901 aaaatctccc acgccatacc cacgtcagta aagaccacac cgtaattcag aaagagaaaa
     3961 cggcgagcaa attcaacgca ttgccacttg tggcccatat attcgtcgtc gatatagcta
     4021 cggaatacgg cgtcatcttc gtattcctgc ggatcgagag aactgtaatc tgaagagtag
     4081 attgctaccc cacctggggc gtagcccaat aatgtcccga acggggcatc ctggctggtc
     4141 gttcctttgc tcatcacttt acctttaaca atacaaccta agcagttggc gtaattgttg
     4201 tgctctgatt acctgctcag cgacattaac cggacagagg tcagactaat catacacctc
     4261 atcgcagcgg ctggcgcaaa gggttaaaaa attcacattc tgtacagcaa gtgacctgct
     4321 acactgcttc aacactacca ctcagaaggc aactcactat gacagacaat acttatcagc
     4381 ccgcgaaagt ctggacgtgg gataaatccg ctggcggcgc gttcgccaat atcaatcgcc
     4441 cggtttctgg tccgacgcat gaaaaaacgc tgcccgttgg caaacaccca ttgcaacttt
     4501 attcgctggg aacgccgaac ggtcagaaag taacgattat gcttgaggag ctgctggcgc
     4561 tgggcgttac tggtgcagag tacgacgcct ggctgattcg tattggcgat ggcgatcaat
     4621 tctccagcgg ctttgtcgaa gtgaacccaa actcgaagat cccggcgctg cgcgatcata
     4681 cgcataatcc gccgatccgc gtgtttgaat ctggttcgat cctgctttat ctggcggaga
     4741 aatttggcta cttcctgccg caggatttgg caaagcgtac tgaaacgatg aactggctgt
     4801 tctggttaca gggcgcggca ccgttcctcg gcggtggttt tggtcacttt taccattacg
     4861 cgccgatgaa aattgagtac gccatcaacc gctttaccat ggaagccaaa cgtctgctcg
     4921 acgtgctgga taagcaactg gcgcagcata agtttgttgc gggcgatgag tacaccattg
     4981 cggatatggc gatttggccg tggtttggca atgtggtgtt aggcggcgtg tatgatgccg
     5041 cagagtttct tgatgcgggc agttataagc atgtacaacg ctgggcgaaa gaagtaggcg
     5101 aacgtccggc ggtgaaacgt gggcgtattg ttaaccgcac caacggaccg ctgaatgagc
     5161 agttgcatga gcgccatgac gccagtgatt tcgagacgaa tacggaagat aagcgtcagg
     5221 ggtaagggtt ggtgttcgtc gcagcaagcc atccaggccg gataaggcgt tcacgccgca
     5281 tccggcaatc gtgcacaatg cctgatgcga cgctgccgcg tcttatcagg cctacgaaag
     5341 ctaatcatca cgcgctggta atgtcgtaat ccatttggcg cagtgcgtct aatgtggctt
     5401 tggcttcatc ttcgtcgatg atgctcatgg caaatccgac gtgtaccagc acccactggc
     5461 ccagtagatc ggcagggtta ccttcacaaa tcagggcgat attcacgtcg cgcttgatac
     5521 cacatacttc aacctgcgca agctggtgaa tatcttcacc gacagccagc acctggcctg
     5581 gaacgccaat acacattatt aactccggtt attcaacttc aatacttttg acgatcagcg
     5641 aatcgccggt atcgacacgc aaccgctcgc cgtgacagag cggacactgc gcatcgtgct
     5701 gatgaatctc caccacctgg ctgcaatccc agcaccaagc ctgggcgggt ttatagacga
     5761 tatgtaaatc gcacccttgc gccaccgttc cgtggcagac aatttcaaaa ctaaaacgga
     5821 cggcgctctc ctcaacgcag gagagcgcgc caatttccag ccacacggcg gtgacgcgct
     5881 taacatcgtg ctgctccgcc tgccgttgga taatttcaac ggcgctctgg caaagagaca
     5941 actcatgcat tttcgccact cctgcgacca aacagcaggg cgcgacgccc tgcgtgtggt
     6001 acatccggat tcgtgactgg cagcgaaagg atcattcgtg cgcagtcatc cgtcaggcgt
     6061 tggccctctt caatcgacat gctgtgcgaa agcggcgaca tcagcgaaca ggagagatat
     6121 tgcgaaacac cgtccagttc gccaacggta aaggtcatag tgccatacgg cagttgcaga
     6181 ccaatttttt cactcacttt gcgcagcggc cagagttgat ccgggccggg gaagataact
     6241 gcactcagca tccacggggt gatcacacac cccgtccact gaccttcgaa cagcgtaaaa
     6301 tcagaaacat acaccggcat tgaaggatgc agaaaagaga gatcgtgcat cgaacgccgg
     6361 gcaatttctt caaacgctgc ctgtacttgc gccttcgggg aggtctggaa acctgctatc
     6421 tcttcagtca tgaatcgcct cccgtgggat ggcttccacg cctgattcgc gcagcgcagc
     6481 cagaacctgc tcaagcgcag gttcaatcat tgcttcaacc gtcggcgtta agccgatgtg
     6541 tggctccagc gattccggga tcacgccgac cagggtcagc tttttcggaa actcaccggt
     6601 gaagcgcagg gccgacaata cgtcggccag gccaagctga tgcggagaga ttttgttggt
     6661 aaacaacgcc ggaacttctt catcccgcag gatcatcatc gttcccggcg tgttcttttt
     6721 cgacacaatg gcatccgcaa taatcagatg atcgcgattt gccatgtcgc caagcagctc
     6781 cattcccgcc gtgccgccat cgaggatctc aacataatcc ggcagaatgt atcgttgctc
     6841 taacgcttcg acaatccgca caccgatggc ttcatcggtc agcaaaatat tgccgacccc
     6901 taagactaaa atacgcatta cagaaccttc actgaaacca cttcgttgcc gtcggcatcc
     6961 actacgtgta ccgcacaggc catgcacggg tcgaaggagt gaatggtacg caccacttcc
     7021 agcggtttat tcggatcagc aaccggtgta cccaccagcg actgctcgta aggaccgacg
     7081 tcatcattga agttacgcgg accagagttc caggttgatg gaacaactgc ctggtagtta
     7141 ctgataatac cgtctttaat aaccatccag tgagagagca taccgcgcgg cgcttcgagg
     7201 aagccaacac ctttgaactc acccgttgcc ggaatgttcg gtttcacaaa ggtggtgtga
     7261 tcgcctttgc cgatattggt gatcagtgca ctgtattgat tttgcaggat atcctgcaat
     7321 tcgcagcagt gaacggtacg accaataata cggcccagcg tggagtgcag ctgcgccact
     7381 tccagcgtgt tgccagtcag tttctgataa atcgcaacga tttcattcag tttgttctgg
     7441 gtagattcgc gacctgccgc cagtttcacc agcatattag ccagcggacc cacttctacc
     7501 gttttgccgt agaaagtcgg tgatttcacc caggaatatt tcccgtcgtc agaccaacca
     7561 tcataagccg gaatggtggt accttcccac ggtgcctgcg gcgcttcgtc tttataccag
     7621 gagtgcttcg cgctttcctg aatgcctttg atcaggtatt catcggaatg agaagtgatc
     7681 ggacgatacg aggacagatc cgcattctca atgtagccgc ccgggaacag gaagctgccg
     7741 tttttactgt cggtcgggaa ttccggcacg ctcaggtagt tcaccgcacc tttaccgcgc
     7801 gtcagccatt ccgggtagaa cgcggcaata actgcggtat caaccttata aacctgttca
     7861 acaaagtcgc tcagcttgtc gatgaaagac ttgatgtaca tcaggcgctc aaggttcagc
     7921 acgcccaaac cgtcgaggtt gatcgggttc gcgacaccac ctaccgccag gttctgaatg
     7981 tgcggcgttt taccgcccag cagcgccacg acgcggttag cgtcacgctg gcactccagc
     8041 gcctgcaggt agtgcgctac cgcaatcagg ttcacttccg gcggcagttt catcgccggg
     8101 tgaccccagt agccattggc gaaaataccc aactgaccgc tggcaaccag atctttgatc
     8161 ttgttctgaa ctttagtgaa ctcttccgga ctgttcagat gccaggtcga aacgcctttc
     8221 agcatttcgg aggctttggt tgggtcagct tgcagtgcag aagtgatgtc cacccagtcc
     8281 agcgccgaaa gctgatagaa atgaacgata tggtcatgcg tggtgtgcgc agccagaatg
     8341 atgttacgga tgtattgcgc gttaaccgga acgtcgatat tcagcgcact ttctgccgca
     8401 cgaacggaag acagcgcgtg agtggtagta catacgccac agatacgttg cacaatcatc
     8461 catgcatcgc gcggatcgcg gtttttcacg atctcttcca tgccgcgcca catggtaccg
     8521 gaagcccatg ctttcgaaac gacgccattt tcgatttcgc aatcgatgcg taaatgcccc
     8581 tcaatacggg ttaccggatc aatagtaatt ctctggctca tgctttgctc gcctcatgac
     8641 gattatgatc gttttgtttt aaaggaggaa gtatcggcag tagacgaatg agtacgatgt
     8701 aagcgcaaat ctcaatagcc acaaaaccaa tagaaatcaa cagttcttcc caggtcggga
     8761 agtaggcgta accgccgccc gggttgaatg ccaccagcga ataggtcaga cgccaggttg
     8821 cacaacctaa cagtgcgctc agtgctgaca ggaacagcat gcgggaatca ttacgcagct
     8881 tcgccacacg cagaacgacc agcgggaaga gcatcagcag gacttcaatc cagaacatca
     8941 cggagtagaa gtcaccggca aacgctaacg acagcttgtc gcgatagata agctcgccaa
     9001 agcgcagcac gatgaaaatc gccagcaaca cactgatggt gttggtcagc ttgacgaaca
     9061 gactcttttc atccggaccg ttgccacgca gacccgcctg caccagcgaa ccttcaaaga
     9121 tgacaatcga gaagcccatg atgaacgccg tcagcagcga gaacagcggc aacatttcat
     9181 agctctgcca caacggatgc accttgtagc ccgccgagat catcagtgac cccattgaag
     9241 actggtgcat ggtcggcaac agcgcgccaa gtgcaatgat gaagaacata accttgttca
     9301 gacgcttgag agaaaccttc cagcccagac gttcaaacag tgccggagca aactccagtg
     9361 ccatcacgcc gatatagatg gtcatacaga ccgccgtctc gaacagtacc gagttcacgt
     9421 tgaagtgacc cggaatgtag aagtacggca ggttccagta gcgacccacg tcgatagtga
     9481 tcgacaagcc acccagtgag taaccaaaca gactcgccaa cagcgccgga cgcaccagcg
     9541 gatggtattg cccacggtta aagacgtata ccgcccacgc cagcgcccag ccgccacagg
     9601 caaagccggt gccaatcagc aggtcaaacg cgatccacac gccccacggg aagccgccgt
     9661 tcaggtcaga gacagagccc agaccgaaca ccagacgctt cacaatcagg agcatacaga
     9721 tgacgattaa cggtccaaaa atcatgaccg gtttactgat gattttgccg cccagcggtt
     9781 gtggatcatg actcatgatc gtctcctccg tcgtgatggt cgtttttggt gttgcgacga
     9841 accagcacgg ttaagcccgc cagcacagcc agtggtagca tcatgccttt atacagggtg
     9901 tgttgaatat tttcggaacg cgcaccggta gaaagatcgt ccagtttcgg caggtcgaga
     9961 ttttcataag gcacacccgt cagtaccaga acctgagtac cgccgccctc tttctcgccg
    10021 tacagatgcg gataatattt cggcaccgta tgcaggtaag tgtcgccaga tttcagcgtc
    10081 tgacgcggat agtggtattc gctgccaggc ttcagcgcca gacgtttttt cgcctccgcc
    10141 atcagctctt cacgcgtacc gaaaatcacc gcgcccgccg ggcacacttc tacgcagcca
    10201 ggcagaccgc ctttatcgag acgttccaca cctttctggt tgcacagctc gcacttatgc
    10261 agcgcaccaa acgggttgtt gtagtcgtac ttcggcacgt tgtacggaca ggcgaccatg
    10321 cagtaacggc aaccggtgca cacgtctttg tcgtaatgga caatgccggt tttcggatct
    10381 tttttcagtg cagagaccgg gcacacagag acacagttcg gatcgacgca gtgcatacac
    10441 tgtttcttaa tgtacgcgta gccgttctcc tcctggtctt tgttgacccc tgtgccgctg
    10501 gtccacacct gaatgatgtt attggtatac ggcgacagtt tgtcgttgtt cgaccaggtc
    10561 tgttcccctt gcgggttacg ttcagggaag ttgatatcct gacacttggt gacgcaagcc
    10621 tggcagccta cgcacaaggt cgagtcgtac aacatcccca gcgatcccgg aattggcggg
    10681 cggttttcag cagccgcatg actgacagac ggcagcgcgc ccgtcagcaa tgccccgcag
    10741 gaggctgctt taataaaatt acgtctgttc acggttattc tccccgtgag tcagcgttat
    10801 ctttcttttg ctgacgaccc agttcacgca ccgccatcac gctgacaccg gcaaccagcc
    10861 caaccacacc gccgagcaaa ccaatagcgc ctgcagagac gttgccgccc tctttagcgt
    10921 taacatccgg tttctgtgaa cgcggagtct gattttcgac gttggcaagc tgatggatgc
    10981 ctttatggaa gccgatacct tcttcgttac agccatagca tgggtgacca atcgctaccg
    11041 gccacacgcc gccaacatcg cagaattgca gcgttgagca gttgccgtaa gtttctggcc
    11101 ctttacagcc gaggtggtac aggcaccagc cttcacggtg gccttcatca ccgaactctt
    11161 tggcaaaacg accagcatcg aagtgcgggc gacgttcgca gtgttcgtga atcagacggc
    11221 cataggcgaa ggtcggacgg tttttgtcat ccagtttcgg cggtttgccg taagtgatga
    11281 tgtgcgcaac ggtcgcgagg aagttgtgcg ggttcggcgg gcagcccgga atattgataa
    11341 cggttttgcc tggcagaact tcttgcaggc tgactgcgcc agttgggtta actccagctg
    11401 cggcaacacc gccccacgca gagcaggaac cgatagcgat aatcgctgct gcgccttctg
    11461 ccgctttgcg gatgtgatcc acaatcggct caccagcaac catgcaataa ataccgttat
    11521 cttttaatgg gatggaacca tccacaacca acacatactg ccctttgtac ttctcaagtg
    11581 cgttatgttt gttctcttcg acctgatgac cgaaggcggc ggaaagcact tcgtgatact
    11641 ccagagagat agtctccagc acgaggtttt ctaccgttgg atgcgttgca cgaagcagag
    11701 attccgtaca accggtgcac tcctgcgcgc caatccagat aactggcgga cgctgcgggt
    11761 tagtaaccga ttcggccatc tctgcagcgg ctttgctact taaccccatg gtggcggcta
    11821 atgctgcaca aagcttcatg aaatcacgac ggttaatgcc gtgagaatgg atgagggtgt
    11881 tatctccagt catttatagt tattccgttg cgaagacctg gcatatattt tgcctcaatc
    11941 gcaaaatcaa taatgcgatc gatgcgccat ttaccacaca tttattatgg ttatcgatat
    12001 catgatactg cggcggggcg ctaaaacgaa gggaagaagc attagtgtaa ttaattaaag
    12061 caagataata cgtatgtttg atcaattttc gaggcgataa ccggccataa aaaagcgcaa
    12121 cgtagaacag gaattattct cacgaaagaa atccattacc atctctttat ccagtccata
    12181 gcggcgcgtc agaatacccg cttcccaggc gctaagctgg cgatctccgg tcttttcgta
    12241 catccggtgt gagtagccta atacaaaacc gcgtttataa tcggcacaga attttgtcac
    12301 attaaccgca ctgtcggcat gcgttgcttt taatcccgcc attaagcctt taccaaaatg
    12361 gttattcatc gttcacctca ttcgctatat atcgccatat atagcgatat tttaggcaaa
    12421 gaacgacttt tgcgcacgct attttttcat gcttgtttaa gacgtaatcc agtaagagat
    12481 aaacctatca ggagagatat ttatcgaacc atttcagtgt cctttgccag gcaagatcgg
    12541 cggcagattt gtcataacgg ggcgtggaat cattatggaa tccgtgatta acccccggat
    12601 agatatacgc ctcataaacc ttattattgg ctttcaacgc cgcctcgtaa gcaggccagc
    12661 cctcgttgat tcgggtgtcc agttccgcga agtggagtag taaaggcgct tcaatcttcg
    12721 ccacatcggc agtgggtgcc tgacgaccat aaaacggcac cgcgcaggcc agttccggat
    12781 acgcgacagc cgccgcgttc gatacgccac cgccatagca aaatccggta atacccactt
    12841 tgcctgccgc ttgtggatag cgttgcataa actcaattgc ggcaaagaaa tcattcatca
    12901 gtttggttgg atcaacctgc tgttgcagct cacgaccttt atcatcattt cccggataac
    12961 ctccaacgga acttaagccg tcaggtgcca gggcgatata ccccgccttc gccactcgcc
    13021 gtgccacatc ttcgatatac ggattcagtc cacgattctc atgcaccacc accacggctg
    13081 gcgttttgcc gctcattttt gcgggtttca ccagataacc ccgtacctcg ccgtgaccat
    13141 ttggcgaagg atacatgatg tactcagcaa caatctccgg gtcggtaaac tctacctgag
    13201 tcgccagcgc ataatttggc ttgagcaaat caaacaacgc taatgccgtc atcccgccca
    13261 ccgcatactt cgccgcaagg ttgaggaact cacgtttcga aattttcccg tgagcgtaat
    13321 agtcgtagta atccaacaac tcttgtggga aatctttggc ggttaaacgc ggcatcgttg
    13381 cactcctcag ttggtgtttt tttaagcaaa gcataagcac gtgtttttgc ccagtttttc
    13441 gtcactctgt gagccagact acgggatacg cgctggcgaa tcgctaaact agaaacattg
    13501 tttcgaaatt gaacggtgga aaggagaggt catggtctgg ttagcgaatc ccgaacgtta
    13561 cgggcagatg caataccgct attgcggaaa aagcggttta cgcctgcccg cgttatcgct
    13621 cggtttatgg cacaatttcg gtcacgttaa cgcgctggaa tcacagcgtg cgatcctgcg
    13681 taaagcgttt gatttgggca ttacgcactt tgatttagcc aacaattacg ggccgcctcc
    13741 aggaagcgca gaagagaact ttggtcgcct gctgcgggag gattttgccg cttatcgcga
    13801 tgaactgatt atctctacca aggctggcta cgatatgtgg cccggccctt acggctctgg
    13861 cggttcacgt aaatacctgc tcgccagcct cgaccaaagc ctgaagcgta tagggcttga
    13921 gtatgtcgat atcttttact ctcatcgcgt cgatgaaaat acgccgatgg aagaaaccgc
    13981 ctctgcgctg gctcatgcgg tacaaagcgg taaggcgctg tatgtcggga tctcctctta
    14041 ctcgccagag cggacgcaaa aaatggtcga gttgctgcac gagtggaaaa ttccgctttt
    14101 aattcatcaa ccttcgtaca atttactgaa ccgctgggtg gataaaagcg gcctgctgga
    14161 taccctgcaa aataacggcg tgggctgtat tgcctttact cctctggctc agggattgct
    14221 gaccggaaaa tatctcaacg gcattccgca agattcacgg atgcatcgtg aagggaataa
    14281 agttcgtggt ctgacgccga aaatgcttac cgaagccaac ctcaacagcc tgcgcttatt
    14341 gaatgaaatg gcacagcagc gtggacaatc aatggcgcaa atggcgttaa gctggttgct
    14401 gaaagatgat cgcgtgacgt cggtattgat tggtgccagc cgcgcggagc aactagagga
    14461 gaacgtgcag gcgctgaata atctgacatt tagcaccgag gagctggcgc agattgatca
    14521 gcatatcgcc gatggcgagc tgaatctgtg gcaggcgtcg tccgataaat gacctgttaa
    14581 taacgggcgc gcgaaccgcg cccgaatcag atcagtgatt atgacgagtc agtcggtcaa
    14641 gatagcccat cacaaatgca gaaagcacaa acgtcagatg gataatgacg taccacatca
    14701 gtttgttatc cgggacattt ttcgcatcca taaagacgcg cagtaagtga atggaagaaa
    14761 ttgccacaat cgacgctgct actttgtttt tcagcgacgt tgcgtccatt ttccccagcc
    14821 agttcagctt ctctttgttc tcggagatat ccagctgtga gacgaaattc tcataaccgg
    14881 aaaacatcac catcaccagc aaaccgccaa ccagcgtcat atccaccagc gacagcaaca
    14941 cgaggatcaa atctgattcc gccatcgaga agatattcgg cagtacgtga ataatctcct
    15001 ggaagaactt cagcgccagg gcaactaacg ccagcgaaag gccaaagtac acgggggcaa
    15061 gcagccagcg agaagcatac attgcatttt caagaaaacg ttccatacat tcctgtcgtg
    15121 tagttaaaac gggcaacagt atatcgcaat tgagtaaaca gcacgcaaca gcaaatcaac
    15181 atatacgtta aacctttgtc gcataattct gaggtttagg gatcttcctg gttcccttat
    15241 caacgcatcg agagcgtcta cccttaaagg acgttgaacc tgaagggaga aaaacgatgt
    15301 ctcatttaaa agacccgacc acgcagtatt acactggtga atatcccaaa cagaaacaac
    15361 cgacgccagg catccaggcg aagatgacac cggtaccgga ttgcggcgag aaaacctatg
    15421 ttggtagcgg tcgcctgaaa gatcgtaaag cactggtgac agggggcgat tccggaatag
    15481 gtcgcgctgc cgccatcgct tacgcgcgtg aaggggctga cgtggcgatc agttatcttc
    15541 ccgtggaaga agaagacgct caggatgtga aaaagatcat tgaagaatgc ggacgcaaag
    15601 ccgttctgct gccaggcgat ttaagcgatg agaaattcgc ccgttcgctg gttcacgaag
    15661 cgcacaaggc gttaggcggg ctggatatta tggcgctggt cgccgggaaa caggttgcca
    15721 ttccggatat tgcagacctc accagcgaac agtttcaaaa gacctttgcc attaacgttt
    15781 tcgcgctgtt ctggctaacc caggaagcga tccccctgct accgaaaggt gcaagtatca
    15841 tcaccacttc gtcaatccag gcataccagc caagtccgca tttactggac tatgcggcta
    15901 cgaaggcggc gattctgaac tacagccgtg gcctggcaaa acaggtcgcg gagaaaggta
    15961 ttcgggtgaa tattgtcgcg ccaggcccta tctggacggc actgcaaatt tccggcggac
    16021 aaacgcagga taagatcccg cagtttggtc agaaaacgcc gatgaagcgt gcggggcaac
    16081 cggcggaact ggcccctgta tatgtttatc tggcaagtca ggagtcgagc tacgtcaccg
    16141 cagaagtgca cggcgtgtgc ggcggcgagc atttaggtta aaaaaatgcc cggttgtgaa
    16201 aagcaaccgg gcatcattgt gaattcactc ttacctgatg tggcgaaaac gccttatccg
    16261 gcctacaaac gcacgaagat tcaacatgtt gcaatagatt ttgtaggcct gatgagcgta
    16321 gcgcatcagg caattctact ttacttcgct ttggcggttt cttcgccgac cagacctatc
    16381 ttcaggtaac ccgcctgatg cagcgtatcc attaccttca tcaacgtctc gtaatcgacg
    16441 gttttgtccg ctcggaagaa gatggtggtg tctttcttgc cttcggttaa cgcattcaac
    16501 gccgtaatca ttgtttcatc ggtgaccgga tcgttaccga taaacatcga gttgtctgcc
    16561 ttcaccgaca gataaaccgg tttttccggg cgcggctgcg gcgtgctggt agaagcaggc
    16621 aagttcacct tcacatctac cgtcgctaac ggtgccgcca ccataaagat aatcagcaga
    16681 accaacatca cgtcgataaa cggcgtcacg ttgatatcat gcatttcgcc gttatcgtcg
    16741 aggttttcgt taagatgcat tgccatcgga cattatcctg cgcgtaattt ttgtgcgaca
    16801 cgaaccggat gcgcagcggc gctggcttcc agatccaggt cacggctttg cagcaacaat
    16861 acctgcgctg caacatcacc cagcatcgct ttaaagccgc caatctggcg tgcaaatacg
    16921 ttatagataa cgaccgcagg aatcgctgcc acgaggccga ttgccgttgc taacagagct
    16981 tctgcgatac ccggcgcaac gactgccagg ttagtggtct gcgtttgcgc gataccaata
    17041 aagctgttca tgatgcccca gaccgtacca aacagaccaa cgaacggaga aatcgcgccg
    17101 atggttgcca gatagccgtt accgcggccc atttgacgac ccactgcggc gacccgacgc
    17161 tccagacgga agctggtacg ttctttaata ccttcgttat cgtcgctgcc ttctgacagt
    17221 tccagctcat tctgcgcttc attgagcaaa tgcaggctta agcttttgct accaaaatca
    17281 gcggcgatat cgttggcctg gtttaaggaa cgcgcttcag ccagcagttg ctgctcgcgc
    17341 ttaaggcgac gcttctgatt gaagaactct acgctcttac tgaagaagat tgcccaagtg
    17401 actacggagg ccaaaataag cccaatcatc acgcacttaa cgacaatatc ggcgtgctga
    17461 tacatacccc agacggaaag gtccgtctgc atcaaattat tacccactgt gtatctccag
    17521 gacgcaagtc acaaaatctg cgcataataa tatcaaaatg acgtcgaatt gatagtcgtt
    17581 ctcattacta tttgcatact gccgtacctt tgctttcttt tccttgcgtt tacgcagtaa
    17641 aaaagtcacc agcacgccat ttgcgaaaat tttctgcttt atgccaattc ttcaggatgc
    17701 gcccgcgaat attcatgcta gtttagacat ccagacgtat aaaaacagga atcccgagat
    17761 ggcggacaaa aagcttgata ctcaactggt gaatgcagga cgcagcaaaa aatacactct
    17821 cggcgcggta aatagcgtga ttcagcgcgc ttcttcgctg gtctttgact gtgtggaagc
    17881 caaaaagcac gcgacgcgca atcgcgccaa tggcgagttg ttctatggac ggcgcggaac
    17941 gttaacccat ttctccttac aacaagcgat gtgtgaactg gaaggtggcg caggctgcgt
    18001 gctatttccc tgcggggcgg cagcggttgc taattccatt cttgcttttg tcgaacaggg
    18061 cgatcatgtg ttgatgacca acaccgccta tgaaccgagt caggatttct gtagcaaaat
    18121 cctcagcaaa ctgggcgtaa cgacatcgtg gtttgatccg ctgattggtg ccgatatcgt
    18181 taagcatctg cagccaaaca ctaaaatcgt gtttctggaa tcgccaggct ccatcaccat
    18241 ggaagtccac gacgttccgg cgattgttgc cgccgtacgc agtgtggtgc cggatgccat
    18301 cattatgatc gacaacacct gggcagccgg tgtgctgttt aaggcgctgg actttggcat
    18361 cgatgtttct attcaagccg ccaccaaata tctggttggg cattcagatg cgatgattgg
    18421 cactgccgtg tgcaatgccc gttgctggga gcagctacgg gaaaacgcct atctgatggg
    18481 ccagatggtc gatgccgata ccgcctatat aaccagccgt ggcctgcgca cattaggtgt
    18541 gcgtttgcgt caacatcatg aaagcagtct gaaagtggct gaatggctgg ctgaacatcc
    18601 gcaagtagcg cgagttaacc accctgctct gcctggcagt aaaggccacg aattctggaa
    18661 acgagacttt acaggcagca gcgggctatt ttcctttgtg cttaagaaaa aactcagtaa
    18721 tgaagagctg gcgaactatc tggataactt cagtttattc agcatggcct actcgtgggg
    18781 cgggtatgaa tcgttgatcc tggcgaatca accagaacat atcgccgcca ttcgcccaca
    18841 aggcgagatc gattttagcg ggaccttgat tcgcctgcat attggtctgg aagatgtcga
    18901 cgatctgatt gccgatctgg acgccggttt tgcgcgaatt gtataacatt gccacttttg
    18961 gacaattttg cagacattta attgtgaaaa gtcttaaatt gttgcgtccg ggatcaaggc
    19021 gtcccggacg aatcaggagt acaataggca gataaaggct taaacgctgt tccacaggaa
    19081 agtccatggc tgttattcaa gatatcatcg ctgcgctctg gcaacacgac tttgccgcgc
    19141 tggcggatcc tcatattgtt agcgttgttt actttgtcat gtttgccacg ctgtttttag
    19201 aaaacggcct gctgcccgcc tcatttttgc caggcgacag cttgttgata ctggcaggcg
    19261 cattgattgc ccaaggggtt atggattttc tgcctacgat tgcgattctg accgccgcag
    19321 caagtctggg ctgctggcta agttatattc aggggcgctg gttagggaat accaaaacgg
    19381 tgaaaggctg gctggcacag cttcctgcta aatatcacca gcgcgccacc tgcatgtttg
    19441 accgccacgg tctgctggcg ctgctggctg gacgttttct tgcatttgtc cgtacgctgc
    19501 tgccaaccat ggcgggaatt tccggtctgc caaaccgccg cttccagttt ttcaactggt
    19561 taagtggatt gctgtgggtc agcgtggtaa ccagttttgg ctatgcctta agtatgattc
    19621 cgttcgttaa acgccatgaa gatcaggtaa tgacgttcct gatgatcctg ccaattgcct
    19681 tgttaaccgc tggcttgtta ggcacgctgt ttgtagtgat taaaaaaaaa tactgtaacg
    19741 cctgacgatt ttccccgttc ccggctgctg taccgggaac gtatttaatt cccctgcatc
    19801 gcccgcattc ttgccgcatc ttcccccggc gtcacaccga agtaacgttt aaactcacgg
    19861 ctaaattgcg atgcgctttc atagtcgacg cgcatcgctg ctgcgctggc cttcatgccg
    19921 tcatggatga tcatcatccg cgccttatgc agacggtaat tcttcaaata ctgcaacggc
    19981 gaggtgctgg tgacagactt aaaattatga tggaacgccg atacgctcat gttggcttct
    20041 gccgccagtt gctcgacgct caggttttcg gtgtatttat tctcaatccg tttcagcacg
    20101 cggctaatca gactgaagtg agtctggcga ctgaccagcg ccagtaacgc gccgccgcaa
    20161 ggtccggtca gcacgtagta cagaatttcg cggatgatct gtttgccgag aatacgcgca
    20221 tccagtggtc gctccatcac gtcgagtaac cgctccgccg cgcataaaat ctcttctgat
    20281 aacgtggcgg agttaatccc gctggctgcc atcgacggct ggaaatgctc atcttcgcca
    20341 atgtccatca acagttcctg taactgcaaa atatcgacat tgagacgcaa tcctgccagc
    20401 ggcacctctg acgtggcata ggtttcgcac tcaaacggca acggcaccgt cagcagcagg
    20461 tattcattgg catcataacg aaacacgcgt tcattgatat aaccgatttt atgcccggaa
    20521 aagagaatta tgatgccagg ctcgtacatc accggtgtac gtgcgaaagg cgtctcgcca
    20581 tacaacaaac gcacatcggg caacagttct gacaaactat tttctttatt tttcagttta
    20641 ttaactttat ccgccagcaa gcggcaaatc tcttcacgtt tcatatcgcg taatttctta
    20701 ggaataatgc ggcaatttga ttgtgcgcaa ttttgtagca tttctccagc actctggaga
    20761 aataggcaag acattggcag aaatgagcat tgagagccag ggcgctggcg atcacaatga
    20821 aaaacatcag gcagatcgtt ctctgccctc atattggccc agcaaaggga gcaagtaatg
    20881 aacaacttta atctgcacac cccaacccgc attctgtttg gtaaaggcgc aatcgctggt
    20941 ttacgcgaac aaattcctca cgatgctcgc gtattgatta cctacggcgg cggcagcgtg
    21001 aaaaaaatcg gcgttctcga tcaagttctg gatgccctga aaggcatgga cgtgctggaa
    21061 tttggcggta ttgagccaaa cccggcttat gaaacgctga tgaacgccgt gaaactggtt
    21121 cgcgaacaga aagtgacttt cctgctggcg gttggcggcg gttctgtact ggacggcacc
    21181 aaatttatcg ccgcagcggc taactatccg gaaaatatcg atccgtggca cattctgcaa
    21241 acgggcggta aagagattaa aagcgccatc ccgatgggct gtgtgctgac gctgccagca
    21301 accggttcag aatccaacgc aggcgcggtg atctcccgta aaaccacagg cgacaagcag
    21361 gcgttccatt ctgcccatgt tcagccggta tttgccgtgc tcgatccggt ttatacctac
    21421 accctgccgc cgcgtcaggt ggctaacgac gtagtggacg cctttgtaca caccgtggaa
    21481 cagtatgtta ccaaaccggt tgatgccaaa attcaggacc gtttcgcaga aggcattttg
    21541 ctgacgctaa tcgaagatgg tccgaaagcc ctgaaagagc cagaaaacta cgatgtgcgc
    21601 gccaacgtca tgtgggcggc gactcaggcg ctgaacggtt tgattggcgc tggcgtaccg
    21661 caggactggg caacgcatat gctgggccac gaactgactg cgatgcacgg tctggatcac
    21721 gcgcaaacac tggctatcgt cctgcctgca ctgtggaatg aaaaacgcga gaccaagcgc
    21781 gctaagctgc tgcaatatgc tgaacgcgtc tggaacatca ctgaaggttc cgacgatgag
    21841 cgtattgacg ccgcgattgc cgcaacccgc aatttctttg agcaattagg cgtgccgacc
    21901 cacctctccg actacggtct ggacggcagc tccatcccgg ctttgctgaa aaaactggaa
    21961 gagcacggca tgacccaact gggcgaaaat catgacatta cgttggatgt cagccgccgt
    22021 atatacgaag ccgcccgcta agctttttac gcctcaaact ttcgttttcg ggcatttcgt
    22081 ccagacttaa gttcacaaca cctcaccgga gcctgctccg gtgagttcat ataaaggagg
    22141 aacgtatggc taatccaacc gttattaagc tacaggatgg caatgtcatg ccccagctgg
    22201 gactgggcgt ctggcaagca agtaatgagg aagtaatcac cgccattcaa aaagcgttag
    22261 aagtgggtta tcgctcgatt gataccgccg cggcctacaa gaacgaagaa ggtgtcggca
    22321 aagccctgaa aaatgcctca gtcaacagag aagaactgtt catcaccact aagctgtgga
    22381 acgacgacca caagcgcccc cgcgaagccc tgctcgacag cctgaaaaaa ctccagcttg
    22441 attatatcga cctctactta atgcactggc ccgttcccgc tatcgaccat tatgtcgaag
    22501 catggaaagg catgatcgaa ttgcaaaaag agggattaat caaaagcatc ggcgtgtgca
    22561 acttccagat ccatcacctg caacgcctga ttgatgaaac tggcgtgacg cctgtgataa
    22621 accagatcga acttcatccg ctgatgcaac aacgccagct acacgcctgg aacgcgacac
    22681 acaaaatcca gaccgaatcc tggagcccat tagcgcaagg agggaaaggc gttttcgatc
    22741 agaaagtcat tcgcgatctg gcagataaat acggcaaagc cccggcgcag attgttatcc
    22801 gctggcatct ggatagcggc ctggtggtga tcccgaaatc ggtcacacct tcacgtattg
    22861 ccgaaaactt tgatgtctgg gatttccgtc tcgacaaaga cgaactcggc gaaattgcaa
    22921 aactcgatca gggcaagcgt ctcggtcccg atcctgacca gttcggcggc taacatgcaa
    22981 attctcccgg tgacggtaat gttccgctac cggacttttc agaaatcatt tattcccctc
    23041 gcgtcccgcc cgttgttact cttccttgtt caggaatgcc aaatataagg acatcatcat
    23101 gcagagccgg aagctcttaa aagaacaact catctatatc cgggataaac gcaacggaga
    23161 ggtgaaaaac agatgaaaat aatacttctg tttttagcag ccctggcaag ttttaccgta
    23221 cacgcgcagc ctccttcaca gaccgtagaa caaaca
//
LOCUS       DAJVQG010000057        23237 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJVQG010000057 DAJVQG010000000
VERSION     DAJVQG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23237
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.057"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(40..240)
                     /gene="glgS"
                     /locus_tag="OQM83_001984"
     CDS             complement(40..240)
                     /gene="glgS"
                     /locus_tag="OQM83_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCS1520255.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            509..1138
                     /locus_tag="OQM83_001985"
     CDS             509..1138
                     /locus_tag="OQM83_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HCS1520256.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            1165..2826
                     /locus_tag="OQM83_001986"
     CDS             1165..2826
                     /locus_tag="OQM83_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCS1520257.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            3065..3124
                     /locus_tag="OQM83_001987"
     CDS             3065..3124
                     /locus_tag="OQM83_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1520258.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3441..3500
                     /gene="ibsE"
                     /locus_tag="OQM83_001988"
     CDS             3441..3500
                     /gene="ibsE"
                     /locus_tag="OQM83_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCS1520259.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3620..5053)
                     /gene="hldE"
                     /locus_tag="OQM83_001989"
     CDS             complement(3620..5053)
                     /gene="hldE"
                     /locus_tag="OQM83_001989"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCS1520260.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKQGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(5101..7941)
                     /gene="glnE"
                     /locus_tag="OQM83_001990"
     CDS             complement(5101..7941)
                     /gene="glnE"
                     /locus_tag="OQM83_001990"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCS1520261.1"
                     /translation="MKPLSSPLQQYRQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7964..9265)
                     /gene="ygiF"
                     /locus_tag="OQM83_001991"
     CDS             complement(7964..9265)
                     /gene="ygiF"
                     /locus_tag="OQM83_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCS1520262.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9507..10127
                     /locus_tag="OQM83_001992"
     CDS             9507..10127
                     /locus_tag="OQM83_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCS1520263.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            10191..11429
                     /gene="cca"
                     /locus_tag="OQM83_001993"
     CDS             10191..11429
                     /gene="cca"
                     /locus_tag="OQM83_001993"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCS1520264.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11592..12413)
                     /gene="bacA"
                     /locus_tag="OQM83_001994"
     CDS             complement(11592..12413)
                     /gene="bacA"
                     /locus_tag="OQM83_001994"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCS1520265.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12504..12872)
                     /gene="folB"
                     /locus_tag="OQM83_001995"
     CDS             complement(12504..12872)
                     /gene="folB"
                     /locus_tag="OQM83_001995"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCS1520266.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12977..13594
                     /gene="plsY"
                     /locus_tag="OQM83_001996"
     CDS             12977..13594
                     /gene="plsY"
                     /locus_tag="OQM83_001996"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCS1520267.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13607..14539)
                     /gene="ttdR"
                     /locus_tag="OQM83_001997"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(13607..14539)
                     /gene="ttdR"
                     /locus_tag="OQM83_001997"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            14746..15657
                     /gene="ttdA"
                     /locus_tag="OQM83_001998"
     CDS             14746..15657
                     /gene="ttdA"
                     /locus_tag="OQM83_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HCS1520268.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15654..16259
                     /gene="ttdB"
                     /locus_tag="OQM83_001999"
     CDS             15654..16259
                     /gene="ttdB"
                     /locus_tag="OQM83_001999"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCS1520269.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            16308..17771
                     /gene="ttdT"
                     /locus_tag="OQM83_002000"
     CDS             16308..17771
                     /gene="ttdT"
                     /locus_tag="OQM83_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCS1520270.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17814..18827)
                     /gene="tsaD"
                     /locus_tag="OQM83_002001"
     CDS             complement(17814..18827)
                     /gene="tsaD"
                     /locus_tag="OQM83_002001"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCS1520271.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            19064..19279
                     /gene="rpsU"
                     /locus_tag="OQM83_002002"
     CDS             19064..19279
                     /gene="rpsU"
                     /locus_tag="OQM83_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCS1520272.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            19390..21135
                     /gene="dnaG"
                     /locus_tag="OQM83_002003"
     CDS             19390..21135
                     /gene="dnaG"
                     /locus_tag="OQM83_002003"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCS1520273.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            21330..23171
                     /gene="rpoD"
                     /locus_tag="OQM83_002004"
     CDS             21330..23171
                     /gene="rpoD"
                     /locus_tag="OQM83_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCS1520274.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5652 a         6332 c         5907 g         5346 t
ORIGIN      
        1 ctaatacaaa cactaaaacg ggccatcagg cccgttatat cagtgctcta actccagctc
       61 tcttgcctgc atcatctgtt ggcaatacca ggcataacgt gcgcaaaacc aggttctatg
      121 ttcttcatcc atgttaccag taacggcaag aatatcgacc gggcgacctt ctgcgtgcat
      181 tctggcaaaa ctacgcgcca ggaaatcgaa attatttaaa gaattaagac tatgatccat
      241 aagcacatcc tccggttatc gcataccttt taaggccata tgctttttct gatttcagta
      301 ttccccaatc agtgtagtgc ctttaaacat aacgtgcgta aatatattgt ccccgatcac
      361 actttttagt gaaaaataaa aaactatatt aaaaaacttt ctcttccaga acttctctaa
      421 catgtgttat tattaacaaa gataaatgta agcaaggaaa ctatcccaaa gtaaatattg
      481 cgtcactaaa tggacattgg agtgacatat gattttattc gccgactata atacccctta
      541 tcttttcgct atttcctttg tactgttgat cggcctttta gaaattttag cgcttatctg
      601 cggtcacatg ctctccggtg ctcttgatgc acatcttgac cattataatt ccataaccac
      661 aggtcatatt agccaggcac ttcattatct caatatcgga agattgccag ctctcgtcgt
      721 tctctgcttg ctggctggtt tttttggtct tattggcatc ctgttgcagc atgcctgcat
      781 catggtctgg caatcaccac tgtcgaacct gttcgtggtt cctgtcagtt tactgtttac
      841 gattattgcg gtgcattata ccggtaaaat agttgcgccc tggattccac gcgaccatag
      901 ttcagctatt acagaagaag aatatattgg tagcatggca ttaattaccg gtcatcaagc
      961 cacttcaggt aatccctgcg aaggaaaact caccgatcaa tttggccaaa tacattattt
     1021 actactggag cctgaagaag gaaaaatttt cactaaagga gtcaaagtgt taattatttg
     1081 ccgactttct gcaacgcgat atttggcgga aaacaatccc tggccacaaa tattgtaatt
     1141 tattaatcaa aaggaaattt aataatggat gatattgtta attctgtgcc ctcctggatg
     1201 tttaccgcga ttattgccgt atgcattctg tttattattg gaattatttt cgccaggctc
     1261 tatcgtcgcg cttcggcaga gcaagctttt gttcgtactg gtttaggtgg gcaaaaagtg
     1321 gtgatgagcg gtggcgcaat cgtgatgccg atctttcatg aaataatccc catcaatatg
     1381 aatactctga agctggaagt cagccgctca accattgata gcctgattac gaaagatcgt
     1441 atgcgtgtcg atgtagtagt cgctttcttt gtacgggtaa aaccgtctgt agaaggcatc
     1501 gccactgcgg cgcagacgtt ggggcaacgc acgctatcac cagaagactt acgtatgttg
     1561 gttgaagata aatttgtcga tgccctccgt gcaacagctg cacaaatgac catgcatgag
     1621 ttacaggata cccgcgagaa cttcgtgcag ggagtacaaa atactgtcgc agaagaccta
     1681 tcgaaaaacg gtcttgagct ggaaagcgtt tcacttacca actttaacca gacctcgaaa
     1741 gaacatttca atccgaacaa tgcctttgac gccgaaggtt taaccaaact gactcaggag
     1801 acggagcgcc gtcgtcgcga acgtaacgaa gttgaacagg atgtagaagt tgcggtgcgt
     1861 gagaaaaacc gtgatgcact ttcgcgcaag ttggagattg aacaacaaga agcgtttatg
     1921 acgcttgagc aggagcagca ggttaaaacc cgtactgccg aacagaatgc acgtattgcg
     1981 gcttttgaag ctgaacgtcg tcgtgaagca gagcagacgc gaattctggc tgaacgacaa
     2041 attcaggaaa cggagatcga gcgcgagcag gccgtccgct caagaaaggt tgaggctgaa
     2101 cgtgaagttc gtattaaaga gatcgaacag cagcaggtca ccgaaatcgc caaccagacg
     2161 aaatcgatcg ctattgccgc caaatcggaa caacagtccc aggcagaagc gcgtgctaac
     2221 cttgcactcg cagaagcagt aagtgcgcaa caaaacgtag aaaccactcg ccagaccgca
     2281 gaagccgatc gtgctaaaca agttgcccta atcgctgccg cgcaggatgc agaaaccaaa
     2341 gcggttgaac tgaccgtgcg ggcgaaagca gaaaaagaag ccgcagagat gcaggcggcg
     2401 gctatcgttg agttagccga agctacacgt aaaaagggtc tggcggaagc agaagcacaa
     2461 cgtgcgctga acgatgctat caacgtactt tctgatgaac aaaccagcct taaattcaaa
     2521 ctggccttgt tgcaggcgct acctgcggta atagagaaat ccgttgagcc gatgaagtcc
     2581 attgacggta tcaagattat tcaggtcgat ggtctgaatc gtggcagcgc tgcgggtgat
     2641 gcaaacacgg gtaatgtggg gggcggaaac ctagcggaac aagcattatc agccgctctc
     2701 tcttaccgca cacaggcacc gctgattgac tccttgctca atgaaattgg cgtttcaggc
     2761 ggctcactgg cggcattgac ttcaccctta acctcaacaa ctcccgtcgc cgaaaacgta
     2821 gaataaattt tcagctatga acgtccctgg cacaaaaagc cggggatttt ttatatctgc
     2881 gttccgctaa aaactgcaaa tgatcaggcc gttgcaacgt tttgcgtgac cgctcgggga
     2941 aggcaaaact gcctctggga aagcgttgcg cgaggtccgg cgctcatcaa caatcgggga
     3001 gtcagcaaag ggctgaaacg ggaaagcccc tcccgaggaa ggggccttac agaaggaaag
     3061 ggttatgatg aagctcgtca tcatactgat tgtgttgtta ctcgtaagtt tcgcagctta
     3121 ttaacagcca atcagagggg ggagaaatcc tccctcaccc ttgttccttt actttaggtt
     3181 gaaaaaaaat cagcgtcaag aaggtttcat taacacatat ccctggccca aaaagccggg
     3241 gattttttat atctgcgttc cgctaaaagg tgcaaatgct caggacgttg cagcgttttg
     3301 cgtgatcgct cggggaaggc aaaactgctt cagggaaagc gttgcgcggg gtccggcgct
     3361 catcaacaat cggggagtca gcaaggggct gaaacgggaa agcccctccc gaggaagggg
     3421 ccttatagaa ggaaagggtt atgatgaagt tcgtcatcat actggttgtg ttgttactgt
     3481 taagtttccc gacttactaa caactgatca gagggggaag aaatcctccc ttacccttgt
     3541 tcctttactt taggttgaaa aaacaacagc gtcaataggc ctgcctgcta caaagcgaga
     3601 tctgtgaacc gctttccggt tagccttttt tatcctgttg gatcttcttg atgatgttag
     3661 tggtcgagca accgtcttcg aagttgagca ccagcacttc gccgccgttg gcccagactt
     3721 ctttactccc ggcgatctct tctggtttat agtcgccgcc cttcaccagt aaatctggca
     3781 ggatcccggc gatcaagcgc tgcggcgtgt cttcttcaaa cgacaccacc cagtcgaccg
     3841 cttccagtgc gcccagcaca atcatacgct gttcgagcgg gttaacgggg cgggaatccc
     3901 ctttcagccg tttggtggag gcatcgctgt tcacagcgac aatcagacgg tcaccctgtt
     3961 tgcgagcatt tgccagatag gagacgtgcc cggcgtgcag gatgtcaaag acaccgttgg
     4021 tcattaccac cttttcgccg cgtttacgcg ctgccgctac ggccagcttc agttcctctt
     4081 cggtcatcac gccaaagccg gtatctgcgc gcccacgcac tgcattttcc agctcgatcg
     4141 gcgaaaccgt ggaggttccc agtttgccga ccaccacgcc agccgccgca ttggcaaaga
     4201 agcaggcttc ttccagcgaa ttacccgctg ccagcgttgc cgccaggacg ccaatcaccg
     4261 tgtcgcccgc accggtaacg tcatacactt cctgcgcctg ggttggcata tgcagcggcg
     4321 ctttacccgg ttgcagcagc gacataccct gttcggaacg ggtcactaac agagccgaga
     4381 gttcgtaatc ggcaatcagt ttcatgccgc gctcaacaat ctcttcttcg gtcttacatt
     4441 taccgacaac agcttcaaat tccgagagat tcggcgttaa cagcgtagcg ccgcggtagc
     4501 gctcaaaatc ggtacctttt ggatcaatca gcaccggaac gcccgcttta cgcgccagtt
     4561 ggatcatctg ctgtacgctt gccagcgcac ctttggcgta gtcagaaagc accagcgcgc
     4621 caatcgaact cagcgcctga ttaatccgct cgtgcagcgg ctgcggatca acgccttcga
     4681 aaccttcttc gaaatccaga cggatcagct gttggttgcg ggaaagtacc cgtaatttgg
     4741 tgatggtcgg atgcgtcggt acagaaacga agtcgcattt gacgttgacg tcggccagag
     4801 atttactcag cgcgcgcgct gcatcgtcaa tgcccgtcaa cccgaccagg cgtgcattag
     4861 caccgagaga agcgatattc atcgccacgt tagccgcgcc gcccggacgt tcttcgatgg
     4921 tattcacttt aaccacgggc accggcgctt ccggcgagat acggctggag ggaccgtacc
     4981 agtaacgatc cagcattaca tcaccaacca ccatcactcc tgcacgttca aactctggca
     5041 gcgttacttt cattcctgtc tcctgagaga ttcaaaattt gcgcgcgata ataccatact
     5101 tcattcttcc accagccact tctgccagct tgcccgcacc agatcacgct ctgcggtgaa
     5161 gcaatccccc gacacatggc ccggcaattc ctgtaatgcc agatgatgaa gttcatcgcg
     5221 cagcgtcgtg taagcacggg tcagcgccat cgcttcctgc tcttccataa tgtcgttttg
     5281 cgccagcagc tccagaatac gcacgttgtc tgaccagcgc gttaacttcg gtttttcatg
     5341 agcgtagcgc aacaccagat attgggtaat aaattcgata tcggtaattc cgccttcatc
     5401 agctttgata tcaaagcgat cgcgatgttt attgccgaga tgagcgcgca ttttctcgcg
     5461 catttcccgc acttccgttt gcagagttct accttcacgc ggcagcgtca taatctcgcg
     5521 acgcactgcg tcaaagtgcg cggtgagctg cggatcgccg tacactaccc gcgcacgcac
     5581 cagcgcctga tgttcccacg tccaggcctc gtttttctga taatcggcaa atgcttctgt
     5641 ggaggtaacc agcattcccg ccgccccgga cgggcgcagc cgcgcatcca cttcgtacaa
     5701 aatgccggaa gaggttcgcg tgctgaacag gtgcataatg cgttgcgcca gacgaagata
     5761 aaactgtcgt ccgtcgattt cccgctcgcc gtcagtcatc gcatccattg ggcaatcatg
     5821 gaggaagatc aggtcaagat cggaactgta gcctaactcc cagccgccca gcttgccgta
     5881 gccgaccact gcaaaaccac gcccttcgcg atcgttgagg tgattcggct taccgtagcg
     5941 ggcaaccatt tgaacccacg cctgctgaac gacggcatct atcatggctt ccgccagcca
     6001 ggttaagtga tcgctcactt tcatcaccgg tagcgtacca gcgatatccg ctgcggcgat
     6061 gcgtaacagc tgcgcctgtt tgaactgacg cagcgcctca agctgttgct cttcgtcatc
     6121 ttccggcacg cgcagcaaat actggcgcaa ctcatcgcgg taggcatcgg tcgccgtcgg
     6181 ctggtaaaga gtgtttggat cgagcaattc atccagcaat aatggataac gcgccagctg
     6241 gctggcaatc atcggcgatg cggcacacag agaaatcaaa tggttaagcg ccgcagggaa
     6301 ttcactgagc aattctaaat aggtggtgcg ggtaacaatc cccaccagca aggcggtaat
     6361 gcgcgacagc gtgacagcag cgtcttcacg cgcacagaca tcacttagca gatgcggcat
     6421 cagatggtcg agcacctgac gtcctcgcgg cccgatggtg cgcttatcca gctctttgcg
     6481 gaaatcggaa atcagcgtta gcacctgttt gcgatcatcc tcgctaagat gcgccagcac
     6541 tggcgtagtg tcatcttcct gcaacgcatc ctgccacagc tcacgccact gttccgacag
     6601 cgactcttcc tgagtttcac tttcgtcgtc gccaatcaat tcattaaaca cccggcgcac
     6661 attgttcata tgtgcggtca gcgccccggt cagttgcggc cagtcagcaa agtccatcgc
     6721 ccacgccagc cgcgcacggt taagctcatc agaaggaagc gtctgggttt gttcgtcgtt
     6781 aatgctttgc agcaggtttt ccagacgccg caggaacaga tacgccactc gcaattgttc
     6841 agcatcgttt tcagaaagca gatgcagcgc agcaatggcg ctgagcgttg gcagtaaaga
     6901 gcacgattgc agcgacggtt cgcgcccgcc gcgaatgagc tggaatacct ggacgataaa
     6961 ttcaatttcg cgaataccac ccgcgccaag tttgatgttg tcggtcaaac cgcgccgacg
     7021 cacttcacgg gcaatcattc ctttcatgtt gcgcagcgac tgaatcacgc tgaaatcgat
     7081 gtaacgacgg aaaacaaacg ggcgtagcat cgcacgcaac tcgttagcat agacgccttc
     7141 gctatcgccc ataatccgcg ctttgaccat cgcgtaacgc tcccagtcgc gcccctgctc
     7201 ctggtaataa tcctccaacg cggcaaagct cagcaccagc gggccacttt cgccaaacgg
     7261 acgcagtcgc atatccacgc gatagacgaa gccatcctgc gttggttgat ccagcacttt
     7321 aatcagccgc tgccccatgc gggtaaaaaa ctgcgcgtta tccagttccc ggcgtccgcc
     7381 ctgcgtacaa ccgtgttccg gccaggcaaa aatcagatcg atatcagagg agaaattcag
     7441 ttccccgccg cccagcttac ccatgcctaa gatcagcagc ggttgcgctt cgccctgcgc
     7501 attgcacggc gttccccact cgcggcagca ggcgtcatac agccagtcac gcgccgcaac
     7561 aatcagcgtc tccgccagat aactgagctg ctgcaatatg ctctcttcag taaccagtgc
     7621 cagcgtttgc gcccatgcga tgcgcaccat aatgcgccgc cggaatagcc gcagctcgcg
     7681 cattaacccg gcttcgtcac tcacattact gagcgcctcc tgcaaccatg cagcgtaatg
     7741 ctgccattca tcggcctgcg gcggttggct ttccagttcc gtcaaccact ctcgatgcgc
     7801 aattatgctg tcctgcacaa aatcactgaa agtaagtact gacttcgcct gtgcacttag
     7861 tggttcctcg gctaaaggct ctggcagccg ctcaacaacg gtctgcctgt actgctgtaa
     7921 cggtgaagag agcggcttca taagcgattt tatccttgcc tgattaacgt tttccgctgt
     7981 gcaaccagaa cggttcctga ttgtttgcct cattacggaa atgctcaatt tcaatgcgtt
     8041 gcccggtcgc aatagcgtga cgcagcccct gccagttctc cagccaggca tgcgcgacga
     8101 caggatcata gtaacccgcc agcaacagta ttgagtcaat gtcacgcgtc aggcgtggca
     8161 actggtcacg gtagagatcg cctaacggct ggcagaaaac gcttttcagt tcagcggcat
     8221 ggtgggaaag atggatatcg gcaaagcgtt tgaaggagtc gctgattttg ccctgcgctt
     8281 tggcatctaa aaatggctgc catgctttgc ttaccaacca ttcggtcaac gccagcttcg
     8341 ccattgccgt ttcggtagag tagaccgccg tcacggcaga aaccgcagaa gcaatggtcg
     8401 cttcgcattg ggtcagcaaa tcacgtaagt gagtgctcgc tttacgcggc acaataccac
     8461 cgaacagcat cagcgtatga cggaccaggc taatggctgc cagcacctgt tctttcgccg
     8521 catcgttacc gcgtacccac agttcttcat gatactgcca ttgcgctaac gccagctcga
     8581 gcgccgcttc cagcccctgt tctacatcgg cttttgccgc aacatgcaaa atggtggtcg
     8641 gtttgatttc acgcgccgga ttgccctgcg ccagatgata gccacgcgcc gctttgctca
     8701 ggctgccctg gcgtaatccg gtttgcgata ccagttggtt cgccagtttc agcaccgcgc
     8761 gcgtgtcgcc gctaagcagt tccagttcca gctcacagat aggttcagca aattcccccg
     8821 ctttcacctc cccctgatcg agagcgattt caattcggct gccatcgacc gccaccagcc
     8881 atttttcgcg ataaaaatcg gtgctgaaca gcggttgtac gcgggaagcg agatcggcag
     8941 gcaattcacc gttcggccag acttccgagg gaaactgcgc caggtcgagc gtcggttcgc
     9001 tcaacgccac gttatattcc gggcgctggt gtaagccgcc tgtcactctt cctgcaactt
     9061 tcatggtcat ctcatagcga ccgttttcgc cacgaatacg taagcccata tcgtgcctac
     9121 gcagccagtt atccggcgtt tcgtagtaaa tattcagcaa ctgcacgggg tcatggtgct
     9181 cgccgcccag cgtattgaga tggtcacgca acgcctcaac ggcactgtga ttaacaataa
     9241 actttaattc gatttcctga gccatggcct ttacttatcg gttatgtcac ataagtgacg
     9301 atgaacggcg aacttaatgc gatctttttt gtcagtagat agtattttgc gccaaattgc
     9361 catgcaacgg gcaatttgac gggcgtaaaa gtttgaagca gtggcaagac ctgacacaga
     9421 ggattccgat tgatgtcgaa tgctttgcgt agcgacactg atgccactac tatcgttcca
     9481 ctttccatga caataacgac agcctgatgc caaaattacg cctgatcgga ttaactttac
     9541 tcgcacttag cgcgactgcc gtctcacacg ctgaagaaac gcgctatgtt tctgacgaac
     9601 tgaatacctg ggtccgtagc ggtccgggag atcattatcg cctcgtgggc acggttaacg
     9661 ccggcgagga agtgacctta ttacaaactg acgccaacac caattatgcc caggtgaaag
     9721 acagctctgg ccgtaccgcc tggatcccgt tgaaacaact tagcactgag ccaagcctgc
     9781 gctcccgtgt gccagatctg gaaaatcagg tcaaaaccct gaccgataaa ctcaccaata
     9841 tcgataacac ctggaatcag cgcaccgcag aaatgcagca aaaagtggcg cagagcgaca
     9901 gcgtgatcaa cgggttaaaa gaagaaaatc agaaactgaa aaacgagctg attgtcgcgc
     9961 agaaaaaggt cgatgccgcc agcgtacagc tggatgacaa acagcgcacc atcatcatgc
    10021 aatggtttat gtatggcggt ggcgtgctgg ggcttggctt gctgctcggt ctggtactgc
    10081 cgcacctgat cccaagccgc aaacgcaaag atcgctggat gaactaaatc gccttcttcg
    10141 ttgcactgtc ttattattag gaattgtgta tttgacgaag ggatgatggc gtgaagattt
    10201 atctggtcgg tggtgctatt cgggatgcat tgttagggct accggtcaaa gacagagatt
    10261 gggtggtagt cggcagtacg ccacaggaga tgctcgacgc gggctaccag caggtaggcc
    10321 gcgattttcc tgtgtttcta catccgcaaa cgcatgaaga gtatgcgctg gcacgtaccg
    10381 aacggaaatc cggttccggt tacaccgatt ttacttgcta tgccgcaccg gatgtcacgc
    10441 tggaagatga tcttaagcgt cgcgatctga ccattaatgc gctggcccag gacgataacg
    10501 gcgagattat cgacccgtac aacggtctgg gcgatctgca aaatcgtctg ttgcgccatg
    10561 tttcccccgc ttttggcgaa gatccgttac gcgtactgcg cgtggcgcgt tttgctgcgc
    10621 gttatgccca cctcggtttt cgtattgccg atgaaactct gacgctgatg cgcgagatga
    10681 cccatgcggg tgaactggaa cacctgacgc ctgaacgggt atggaaagag acggaaagcg
    10741 ccctcaccac ccgcaatcca caggtgttct tccaggtact gcgcgattgc ggcgcactgc
    10801 gcgttttatt cccggaaatt gacgcactgt ttggcgttcc ggcccctgcc aggtggcatc
    10861 cggaaatcga cacgggtatt cataccttaa tgacgctctc aatggcggcg atgctgagtc
    10921 cgcaggtcga tgtccgtttc gcaactttat gccacgatct cggtaaagga ctgacgccgc
    10981 cagaactttg gccgcgtcat catggtcacg gtccggcggg tgtgaaatta gtggaacaac
    11041 tctgccagcg tctgcgcgtg ccaaatgaaa ttcgcgattt agccagactg gtggctgagt
    11101 ttcacgatct catccacacc ttcccaatgc tgaacccaaa aactatcgtc aaattgtttg
    11161 attccatcga cgcctggcgt aaaccgcagc gtgtcgagca actggcgctg accagcgagg
    11221 ctgacgtacg cggcagaacc ggttttgaat cagcggacta cccgcaaggc cgttggttgc
    11281 gcgaagcctg ggaagtggcg cagtcagtgc cgacaaaagc ggtcgttgaa gcgggattta
    11341 aaggtgtgga gattcgcgag gagctgaccc gacggcggat tgcggcggta gccagttgga
    11401 aggaacaacg ttgcccaaag cctgactgag tataaaacta aacgttaaca cgaaccatcc
    11461 cctcgcccct tcggggagag ggttagggtg aggggaacag accagcaccg gtgcgaacaa
    11521 tcacccccac cccggccctc tccctacaga gcgagggaga aaaccaggcc tgctcccgaa
    11581 ccttttctca atcaaaagaa cacgacgtac accgcagccg ccacaataaa gcgataaatg
    11641 gcgaacggga taaacgaaat gcgcttaatc aattgcagga aggttttaat cgctatcagc
    11701 gccaccacaa aagcggtgat aaacccaacg gcaaacatcg agatatcgcc ggttgtcagg
    11761 aagccccagc ttttgtagag atcgagcgcc gttgcgccca tcatcatcgg taccgccagc
    11821 aggaacgaga actcggaagc agcgtaacgg ctcacgccca tcagcatccc acctgaaatg
    11881 gtggccccgg aacgggagaa acccggccac agcgccagac actggaaaca gccaatcata
    11941 aacgcctgac gataggtcat atcatcaaga cccggcgcac gcggctcttt cggcttcagg
    12001 cattcggcgg caatcagcaa caaaccgcca acgaccagtg catacatcac atttatcggg
    12061 ttaaacaatg acttaatcgt gtcgtggaac aacagcccca gtaccaccgc cggaatcatc
    12121 cccagcaaaa tgtggatcag cgttaaacga cctttgcttt ctccttcgtg ctgcaacggg
    12181 cggccaaagt ggatgccaat caggccaaac agacgccgcc agaacatcac tactaccgcc
    12241 agaattgatc ctaactggat cacaacttca aaggttttcg ccgtgtcgcc ctcgaacccc
    12301 aacaagtgac cgacaataat catatggccc gtgctggata ccggcagaaa ttctgtcaat
    12361 ccttcgacca cacccaatat tgccgctatc agcagcgagt gcatatcgct catcaataaa
    12421 cccctaaatt attaaaatgt accgcttgtc cggactactg cagtaagacc aggttataac
    12481 cgtttggttt aacagctgta aaattaatta ttttctttca gattattgcc acgctcaatg
    12541 attacaccaa cattcgccgc ccgcgccatt gcgcctggct tgctgagttt gatgcgcacc
    12601 cacggcgagt tgaagcgtgc taacagcagc tccgccacct cttcagccac gcgttccacc
    12661 agcgcaaaac gcgccccctc gacgtggctg accaccgttt ctgcaatgtc agcgtaactg
    12721 aggcaatccg ccacatcatc acttttcgcc gctttacggt tatcccatgc catttcgata
    12781 tcgaacacta acttctgttc gatggtctgt tcccagtcgt aaacaccaat agtggtgatt
    12841 accgaaagtt gctctataaa tacaatatcc atcacgtcct gcctgctttt tggctaaccg
    12901 gataccactt ccggcgaaat gtgcgtatta tccacagatt catcgttgaa cacgaatttt
    12961 caaaacggaa cagcttatga gtgcaatcgc gcctggaatg atcctcatcg cgtacctctg
    13021 cggctccatt tccagtgcca ttctggtttg ccgcttgtgt gggctgcccg atccgcgaac
    13081 cagcggctcc ggcaatccag gcgcaaccaa tgtgttacgt atcggtggca agggagcagc
    13141 cgtagcagta ctgattttcg acgttctgaa aggaatgttg cccgtctggg gcgcgtatga
    13201 attaggtgtc agccccttct ggttaggctt aattgccatc gccgcctgtc ttggacacat
    13261 ctggcccgtt ttcttcggat ttaaaggagg aaaaggcgtt gctaccgctt ttggtgccat
    13321 cgcacccatt ggctgggatc tcaccggagt aatggcggga acctggttac tgaccgtgct
    13381 attgagcgga tactcgtcgc tgggagcgat tgtcagtgca ctgattgctc cgttttatgt
    13441 ctggtggttt aagccacaat tcaccttccc ggtttcgatg ctctcttgcc tgatcctgct
    13501 gcgtcatcat gacaacatcc agcgtctgtg gcgtcgtcag gagacaaaaa tctggacgaa
    13561 attcaaaaga aagcgcgaaa aggatcccga gtgatttctg gtggatctac atgacctgat
    13621 agccttcatc gggcttgccc agccgttgct ggcaccatgc cgccagaaat tccacgcaga
    13681 cacgtaattt catgctgcga tagagcggct cccggtaaac agcccagata ttggcgcttt
    13741 gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc aacacatccc
    13801 actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact atctcgccgc
    13861 tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg ttccccaact
    13921 cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga cgacttaatt
    13981 cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg cataatattc
    14041 ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca ttaatacgaa
    14101 tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat tcaaaatgca
    14161 cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata tggctgcgcc
    14221 caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg atttacgtga
    14281 cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg taacaacgtt
    14341 ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt gttgccagcg
    14401 tattttcaag gatttggatc cgcttggtga caaatgcagg cgtctgccca agcgtcgccg
    14461 ctgcggcgct aaaactcccg ctgtggacga tttctaccag tacctgcaag tctttggcta
    14521 aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag tgtatttcgc
    14581 aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg ttgattcacg
    14641 aattgttaat tacatattca cggtggcaaa aaatataaaa ccacattttt agtggtagtt
    14701 tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat gagcgaaagt
    14761 aataagcaac aggcagtgaa taagttgacg gagattgtcg ctaactttac cgccatgatt
    14821 tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga tgccgaaacg
    14881 tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa agcgattgac
    14941 ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa agtcggttcc
    15001 cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga agaggcaacc
    15061 gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa caccggcaaa
    15121 aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa tgacgatgcg
    15181 gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc gaaagtgtta
    15241 atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat ctccaccctc
    15301 gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc ggtggaaacc
    15361 gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca tcccaatcca
    15421 aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg gattggtcca
    15481 caagggctga ctggcaacag ttcagtgatg ggcgtacata tcgaatctgc cgcccgccat
    15541 ccgtcaacca tcggcgttgc tgtctctacc ggctgctggg cgcatcgtcg cggcacactg
    15601 ctggttcatg ccgatctcac ctttgaaaat ctgtctcaca cccggagcgc gttatgaaaa
    15661 agatcctgac aaccccgatc aaagctgaag atctgcaaga tattcgcgtc gacgatgtga
    15721 tctacctgac cggtacgctg gtgacctgcc gtgacgtttg tcaccgccgc ttgatcgaac
    15781 tgaaacgtcc gatcccttac gatctcaacg gcaaagcgat tttccacgct ggccccatcg
    15841 tgcgcaaaaa cggcgataaa tgggagatgg tttccgttgg cccgacaacc agtatgcgta
    15901 tggaaagttt tgaacgtgaa tttatcgagc agaccggcgt gaaactggtg gttggcaaag
    15961 gcggaatggg gccgctgacc gaagaaggct gccagaaatt caaggcgcta catgtgattt
    16021 tcccggcagg ctgcgcggtg ctggcggcaa cccaggtgga agagattgaa gaagtgcact
    16081 ggacagagct cggaatgccg gagtcgctgt gggtctgtcg ggtcaaagag ttcggcccgc
    16141 tgattgtctc tattgatacc cacggcaaca acctgatagc cgaaaacaaa aagctgttcg
    16201 ccgaacgccg cgatcccatc gtggaagaga tctgcgagca cgtccattac atcaaataac
    16261 cctcccggag aggctcgccc cctctccttt ttcgcaggca taacacgatg aaaccttcca
    16321 ctgaatggtg gcgatacctt gcgccgctgg cggtcatcgc cattattgct ctaattccgg
    16381 ttcccgcagg gctggagagt catacctggc tctactttgc cgtttttact ggcgtgatcg
    16441 ttggactgat cctcgaaccc gtgccgggtg ccgtggtggc gatggtgggt atatccatca
    16501 tcgccatact ctctccctgg ctgctgttca gcccggagca gctcgctcag ccaggcttta
    16561 aattcactgc aaaatccctc tcgtgggccg tttccggttt ttctaattcg gttatctggc
    16621 tgattttcgc cgcctttatg tttggcacag gctatgaaaa aaccgggctt ggacgccgca
    16681 ttgcgctgat tctggtgaaa aagatgggac atcgcacgct gtttctcggc tatgcggtga
    16741 tgttctccga gctgatcctc gcacctgtaa caccgtccaa ctcggcgcgt ggtgcgggga
    16801 ttatctatcc catcatccgt aacctgccac cgctctatca atcacaacca aacgacagca
    16861 gttcgcgcag cattggctcg tacatcatgt ggatggggat tgttgccgac tgcgtgacca
    16921 gcgccatttt cttgacggcg atggcaccaa acttgctgtt aattggactg atgaaaagcg
    16981 catctcacgc cacgctgagt tggggcgact ggttcctcgg gatgttgccg ctcagtattt
    17041 tgctggtgct gctggttccc tggctggctt atgtgctgta cccgccggta ctgaagtctg
    17101 gcgatcaggt gccgcgctgg gcagagacgg aactgcaggc aatgggcccg ctctgttcgc
    17161 gtgaaaaacg gatgctgggg ctgatagtag gcgcgctggt gctgtggatt ttcggcggtg
    17221 attatatcga tgccgcgatg gtcggttaca gcgtggtggc actgatgctg cttctgcgca
    17281 ttatcagttg ggacgacatt gtcagtaata aagcggcgtg gaacgttttc ttctggctgg
    17341 cctcgcttat caccctcgct accggactca acaacaccgg ttttattagc tggtttggca
    17401 aactgttagc aggcagctta agcggttatt cgccaacgat ggtgatggtg gcgttgattg
    17461 tggtgtttta tctgctgcgc tactttttcg ccagcgccac ggcgtatacc tccgccctcg
    17521 caccgatgat gattgccgct gcgctggcga tgccggaaat cccgctgccg gtgttctgcc
    17581 tgatggttgg cgcggcaatt ggtctgggga gcattcttac accatacgcc accggaccca
    17641 gtccgattta ctacggtagt ggttatctgc caacggcgga ttactggcga ctgggggcga
    17701 tttttgggct gatattcctc gtgttgctgg tgattaccgg cttactgtgg atgcccgtgg
    17761 tgttgcttta agtgaggacg gtgccggaaa ttgctctccg gcacccaata gttttacgca
    17821 gccggtaact ccgccagcgg ccagcgcgga cgcacgctaa cgccgagatc cgccgtcgcg
    17881 cctgctttaa accgcaccat tccggcatag gcgatcatcg cgccgttatc agtacaaaac
    17941 tccggacgcg cgtagaacac ttcgccgcgg cgttttttca tcatttcagc cagcttcgcc
    18001 cgtaacgtgc ggttagcact cacgccgccc gccatgacca gtcgcttaaa gcccgtctga
    18061 tccaacgctc gcttacactt aatcatcaac gtatcgacca ccgcatcttc aaaggcgcgg
    18121 gcgatatcag cacgcgtctg gtcgtcggtg ccgttgtcac gaatcgtgtt cgccgcaaag
    18181 gttttcagac cagaaaagct gaaatccagc cccggacggt cggtcatcgg acgcgggaaa
    18241 acaaagcgcc cggcagtacc ctgagccgcc attttcgaca gtaacggtcc gccaggataa
    18301 tccagcccca gcagcttcgc ggttttatca aacgcttcac cggcggcatc atcgatagac
    18361 tcgccgagca gctcgtactg accaatgcca gtcacgctga ttaactgcgt atgaccgccg
    18421 gacaccagca gcgcgacaaa cggaaattcc ggcgggttat cttccagcat cggcgctaac
    18481 agatgccctt ccatatggtg taccgggatt gccggaacgt tccaggcaaa cgccagagaa
    18541 cgccccacgg ttgcgccaac cagcagtgcg ccgactaatc cagggcctgc ggtataggcc
    18601 acagcatcaa tgtcttttgc cgttaagcca gactccttta gcgccgcctg gatcaacggt
    18661 acggttttgc gcacgtgatc gcgggaggcc agttcaggca cgacgccgcc atagtcagcg
    18721 tgcaatttca cctgactata caattggttg gctaacaaac ctttttcatc gtcgtaaatg
    18781 gcgatgccgg tttcatcgca ggaagtttca atacccagta cacgcatgac ttgttttacc
    18841 tcgctttatt accgcgcagt gtaggaccaa tgcgggttga tgtaaaactt tgttcgcccc
    18901 tggagaaagc ctcgtgtata ctcctcaccc ttataaaagt ccctttcaaa aaggccgcgg
    18961 tgctttacaa agcagcagca attgcagtaa aattccgcac cattttgaaa taagctggcg
    19021 ttgatgccag cggcaaaccg aattaatcaa aggtgagagg cacatgccgg taattaaagt
    19081 acgtgaaaac gagccgttcg acgtagctct gcgtcgcttc aagcgttcct gcgaaaaagc
    19141 aggtgttctg gcggaagttc gtcgtcgtga gttctatgaa aaaccgacta ccgaacgtaa
    19201 gcgcgctaaa gcttctgcag tgaaacgtca cgcgaagaaa ctggctcgcg aaaacgcacg
    19261 ccgcactcgt ctgtactaat tccccgagag cgttgctctc cgatcagacc gagttgtagt
    19321 tgtaaggccg tgcttccgaa aggaatgcgc ggcttatttt cgtttatgaa ttgctaaaaa
    19381 tcggggccta tggctggacg aatcccacgc gtattcatta atgatctgct ggcacgcact
    19441 gacatcgtcg atctgatcga tgcccgtgtg aagctgaaaa agcagggcaa gaatttccac
    19501 gcgtgttgtc cattccacaa cgagaaaacc ccgtcattca ccgttaacgg tgagaaacag
    19561 ttttaccact gctttggatg tggcgcgcac ggcaacgcga tcgacttcct gatgaactac
    19621 gacaagctcg agttcgttga aacggtcgaa gagctggcag caatgcacaa tcttgaagtg
    19681 ccatttgaag caggcagcgg ccccagccag atcgagcgcc atcagaggca aacgctttat
    19741 cagttgatgg acggtctgaa tacgttttac caacaatctt tacaacaacc tgttgccacg
    19801 tctgcgcgcc agtatctgga aaaacgcgga ttaagccacg aggttatcgc tcgctttgcg
    19861 attggttttg cgccccccgg ctgggacaac gtcctgaagc ggtttggcgg caatccagaa
    19921 aatcgccagt cattgattga tgcggggatg ttggtcacta acgatcaggg acgcagttac
    19981 gatcgtttcc gcgagcgggt gatgttcccc attcgcgata aacgcggtcg ggtgattggt
    20041 tttggcgggc gcgtgctggg caacgatacc cccaaatacc tgaactcgcc ggaaacagac
    20101 attttccata aaggccgcca gctttacggt ctttatgaag cgcagcagga taacgctgaa
    20161 cccaatcgtc tgcttgtggt cgaaggctat atggacgtgg tggcgctggc gcaatacggc
    20221 attaattacg ccgttgcgtc gttaggtacg tcaaccaccg ccgatcacat acaactgttg
    20281 ttccgcgcga ccaacaatgt catttgctgt tatgacggcg accgtgcagg ccgcgatgcc
    20341 gcatggcgag cgctggaaac ggcgctgcct tacatgacag acggccgtca gctacgcttt
    20401 atgtttttgc cggatggcga agaccctgac acgctggtac gaaaagaagg taaagaagcg
    20461 tttgaagcgc ggatggagca ggcgatgccg ctttccgcat ttctgtttaa cagcctgatg
    20521 ccgcaagttg atctgagtac ccctgacggg cgcgcacgtt tgagtacgct ggcactgcca
    20581 ttgatatcgc aagtgccggg cgaaacgctg cgaatatatc ttcgtcagga attaggcaac
    20641 aaattaggca tacttgatga cagccagctt gaacgattaa tgccaaaagc ggcagagagc
    20701 ggcgtttctc gccctgttcc gcagctaaaa cgcacgacca tgcgtatact tatagggttg
    20761 ctggtgcaaa atccagaatt agcgacgttg gtcccgccgc ttgagaatct ggatgaaaat
    20821 aagctccctg gacttggctt attcagagaa ctggtcaaca cttgtctctc ccagccaggt
    20881 ctgaccaccg ggcaactttt agagcactat cgtggtacaa ataatgctgc cacccttgaa
    20941 aaactgtcga tgtgggacga tatagcagat aagaatattg ctgagcaaac cttcaccgac
    21001 tcactcaacc atatgtttga ttcgctgctt gaactgcgcc aggaagagtt aatcgctcgt
    21061 gagcgcacgc atggtttaag caacgaagaa cgcctggagc tctggacatt aaaccaggag
    21121 ctggcgaaaa agtgatttaa cggcttaagt gccgaagagc gatcgggaag cccccgacag
    21181 ccgcactgag aggcagcggc aaatatataa gtacgccctc gtaattatcg ttggcggtaa
    21241 acaaccgttg gatttcagcg ttaacggctg aaggacatcg ggtcaatcgc ccaacaccaa
    21301 cctcatgaaa taagtgtgga taccgtctta tggagcaaaa cccgcagtca cagctgaaac
    21361 ttcttgtcac ccgtggtaag gagcaaggct atctgaccta tgccgaggtc aatgaccatc
    21421 tgccggaaga tatcgtcgat tcagatcaga tcgaagacat catccaaatg atcaacgaca
    21481 tgggcattca ggtgatggaa gaagcaccgg atgccgatga tctgatgctg gctgaaaaca
    21541 ccgcggacga agatgctgcc gaagccgccg cgcaggtgct ttccagcgtg gaatctgaaa
    21601 tcgggcgcac gactgacccg gtacgcatgt acatgcgtga aatgggcacc gttgaactgt
    21661 tgacccgcga aggcgaaatt gacatcgcta agcgtattga agacgggatc aaccaggttc
    21721 aatgctccgt tgctgaatat ccggaagcga tcacctatct gctggaacag tacgatcgtg
    21781 ttgaagcaga agaagcgcgt ctgtccgatc tgatcaccgg ctttgttgac ccgaacgcag
    21841 aagaagatct ggcacctacc gccactcacg tcggttctga gctttcccag gaagatctgg
    21901 acgatgacga agatgaagac gaagaagatg gcgatgacga cagcgccgat gatgacaaca
    21961 gcatcgaccc ggaactggct cgcgaaaaat ttgcggaact acgcgctcag tacgttgtaa
    22021 cgcgtgacac catcaaagcg aaaggtcgca gtcacgctgc cgctcaggaa gagatcctga
    22081 aactgtctga agtattcaaa cagttccgcc tggtgccgaa gcagtttgac tacctggtca
    22141 acagcatgcg cgtcatgatg gaccgcgttc gtacgcaaga acgtctgatc atgaagctct
    22201 gcgttgagca gtgcaaaatg ccgaagaaaa acttcattac cctgtttacc ggcaacgaaa
    22261 ccagcgatac ctggttcaac gcggcaattg cgatgaacaa gccgtggtcg gaaaaactgc
    22321 acgatgtctc tgaagaagtg catcgcgccc tgcagaaact gcagcagatt gaagaagaaa
    22381 ccggcctgac catcgagcag gttaaagata tcaaccgtcg tatgtccatc ggtgaagcga
    22441 aagcccgccg tgcgaagaaa gagatggttg aagcgaactt acgtctggtt atttctatcg
    22501 ccaagaaata caccaaccgt ggcttgcagt tccttgacct gattcaggaa ggcaacatcg
    22561 gtctgatgaa agcggttgat aaattcgaat accgccgtgg ttacaagttc tccacctacg
    22621 caacctggtg gatccgtcag gcgatcaccc gttctatcgc ggatcaggcg cgcaccatcc
    22681 gtattccggt gcatatgatt gagactatca acaaactcaa ccgtatttct cgccagatgc
    22741 tgcaagagat gggccgtgaa ccgacgccgg aagaactggc tgaacgtatg ctgatgccgg
    22801 aagacaagat ccgcaaagtg ctgaagatcg ccaaagagcc aatctccatg gaaacgccga
    22861 tcggtgatga tgaagattcg catctggggg atttcatcga ggataccacc ctcgagctgc
    22921 cgctggattc tgcgaccacc gaaagcctgc gtgcggcaac gcacgacgtg ctggctggcc
    22981 tgaccgcgcg tgaagcgaaa gttctgcgta tgcgtttcgg tatcgatatg aacaccgacc
    23041 acacgctgga agaagtgggt aaacagttcg acgttacccg cgaacgtatc cgtcagatcg
    23101 aagcgaaggc gctgcgcaaa ctgcgtcacc cgagccgttc tgaagtgttg cgtagcttcc
    23161 tggacgatta atcggtaggc cggatcaggc gttacgccgc acccggcact agtccctctg
    23221 cacaaacgcc acctttt
//
LOCUS       DAJVQG010000058        23103 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJVQG010000058 DAJVQG010000000
VERSION     DAJVQG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23103
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.058"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..157)
                     /gene="cas6e"
                     /locus_tag="OQM83_002005"
     CDS             complement(<1..157)
                     /gene="cas6e"
                     /locus_tag="OQM83_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCS1520275.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREEL"
     gene            complement(139..885)
                     /gene="cas5e"
                     /locus_tag="OQM83_002006"
     CDS             complement(139..885)
                     /gene="cas5e"
                     /locus_tag="OQM83_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HCS1520276.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(896..1951)
                     /gene="cas7e"
                     /locus_tag="OQM83_002007"
     CDS             complement(896..1951)
                     /gene="cas7e"
                     /locus_tag="OQM83_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HCS1520277.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(1963..2499)
                     /gene="casB"
                     /locus_tag="OQM83_002008"
     CDS             complement(1963..2499)
                     /gene="casB"
                     /locus_tag="OQM83_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HCS1520278.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(2496..4057)
                     /gene="casA"
                     /locus_tag="OQM83_002009"
                     /pseudo
     CDS             complement(2496..4057)
                     /gene="casA"
                     /locus_tag="OQM83_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
     gene            complement(4155..6854)
                     /locus_tag="OQM83_002010"
     CDS             complement(4155..6854)
                     /locus_tag="OQM83_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HCS1520279.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGWPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGI
                     NTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGL
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKE
                     DSSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
     gene            complement(7049..7201)
                     /locus_tag="OQM83_002011"
     CDS             complement(7049..7201)
                     /locus_tag="OQM83_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCS1520280.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(7466..8200)
                     /gene="cysH"
                     /locus_tag="OQM83_002012"
     CDS             complement(7466..8200)
                     /gene="cysH"
                     /locus_tag="OQM83_002012"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCS1520281.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(8275..9987)
                     /gene="cysI"
                     /locus_tag="OQM83_002013"
     CDS             complement(8275..9987)
                     /gene="cysI"
                     /locus_tag="OQM83_002013"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCS1520282.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(9987..11786)
                     /gene="cysJ"
                     /locus_tag="OQM83_002014"
     CDS             complement(9987..11786)
                     /gene="cysJ"
                     /locus_tag="OQM83_002014"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCS1520283.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            12105..12467
                     /gene="queD"
                     /locus_tag="OQM83_002015"
     CDS             12105..12467
                     /gene="queD"
                     /locus_tag="OQM83_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCS1520284.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            12545..13816
                     /locus_tag="OQM83_002016"
     CDS             12545..13816
                     /locus_tag="OQM83_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1520285.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            13807..14067
                     /locus_tag="OQM83_002017"
     CDS             13807..14067
                     /locus_tag="OQM83_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCS1520286.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            14084..14659
                     /locus_tag="OQM83_002018"
     CDS             14084..14659
                     /locus_tag="OQM83_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCS1520287.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGGMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(14807..15667)
                     /locus_tag="OQM83_002019"
     CDS             complement(14807..15667)
                     /locus_tag="OQM83_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCS1520288.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(15664..16443)
                     /locus_tag="OQM83_002020"
     CDS             complement(15664..16443)
                     /locus_tag="OQM83_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCS1520289.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(16421..17830)
                     /locus_tag="OQM83_002021"
     CDS             complement(16421..17830)
                     /locus_tag="OQM83_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1520290.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVDFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(17852..19306)
                     /locus_tag="OQM83_002022"
     CDS             complement(17852..19306)
                     /locus_tag="OQM83_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCS1520291.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(19376..20192)
                     /locus_tag="OQM83_002023"
                     /pseudo
     CDS             complement(19376..20192)
                     /locus_tag="OQM83_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            20510..21787
                     /locus_tag="OQM83_002024"
     CDS             20510..21787
                     /locus_tag="OQM83_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1520292.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIFQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAIASSK"
     gene            21814..23103
                     /locus_tag="OQM83_002025"
                     /pseudo
     CDS             21814..23103
                     /locus_tag="OQM83_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
BASE COUNT         5510 a         6154 c         5698 g         5741 t
ORIGIN      
        1 ggagttcttc ccgacgataa agaaattgcc tttctttgcc gccagggaaa agatcccaca
       61 gccactgatg catcacatat tcaccgcgat ccaccagatg cagcaactgc gcaggcgaaa
      121 gctggccggt atgtaaggtt attcttgaga ggtacatggt tcctccttgc tgagccacgg
      181 cccctgattg acggtgcgtt ccccaaacag ccactgctga cgatttaaag gaacatcccg
      241 gcggcgtaat attttgctcg cgaccaggcc gtcgtgtttc ccttcccacc agcattcatc
      301 ctgaagtttc gggagtgaga ctttcagttt atggaaatga tcctgatact gttggtatgc
      361 attacataag gcatcacacg cgttgccttc gagcagtaac ggcgcaagag gtaatgccag
      421 cggatgactt tttcgcccca gataaagcgg aaaaactgga tggcgtaaac cgtcctgcaa
      481 ctgttcgagg ctgtaaggcg catcggctgt tgttgccacc gccaccatcc accaggcatc
      541 ggtgtagtag tcgcgccggg agataatcgc gctcaaaagc tcagggtcgc tcaactcttc
      601 acgacggctg aaataacgtg ctttacgcac ctcttttggc atctggaccg tgtgataatc
      661 ccgtgcccag cgcgggttac ggctggcgca aaccaccagt gaatagtggc gattaaacgc
      721 gtttaagcgt tcggtatcat cacgccgaat ccctaccccg gcagccagta gccccagcaa
      781 tgctgagcgc gaaggaagtt catgggtatg acgaacttca cctggagcat cgacacccca
      841 ggacgccatc ggcccatgaa gctgaaaaat cagatattgg ctcataagcc gccccttacg
      901 cgcagataaa gtccagcacg tccttcatgc ttccctgttt gttcatcacg tcaaaacttg
      961 cgcattcggt cttctgttcg tagaccgtat tcatgttttc gcgaagcgtt gtaatacgct
     1021 gcaccgccac atctaactga cgtgtgccat taatgggttc atagaaagcc gccgccagag
     1081 agcgtggttg atcggtgcct ttttctgcca gcgcccagga agcataggca cggctggcaa
     1141 agctattttg tttaccggtt ggggaaactt taagcgcggc ttccgtaaaa gcgcgtatgg
     1201 tctgattagc taacgcttcg tcaccgccga ggttttctac cagcagatct ttatcaatgc
     1261 agatataggt gtagaaaagt gctgaaccga atccggtttc tccgagatgc ccggcaccgg
     1321 catcttcaga ggcctggcgc aaatcatcaa cggcggtgaa aaaatcatct tcgacaatcg
     1381 tttcactgac accaaacgca tgcgcgacct ggcaggcggc ttcaacatta aactcaggtt
     1441 tattcgccag catacgacca aacatagcga tatctaccgc tatacggtct ttacgtaaca
     1501 acgcgagatc ttcctctttt ggcgcgcgct tttcttctgc cagttgatga gcaagcgctt
     1561 ttacggcgtc aaattctgcc gggctgatat gaactaattg ttcagtttcg gcgttagtga
     1621 gcggatcttt tggttttttg tcatttttag ctttcccaag ataatcagca atttttgccg
     1681 cccattcgat ggctttttta tcttcaatcc ctttctcaat caggatagtt gccgcctcac
     1741 gcgcaatacg cccactgcga ataccaatat ggcccgccag tgcctgttca aaaagtgcag
     1801 aagtgcgcca cgcacgtttc agactttgcg aggaaacgcg cagacgcgtt gctccaccca
     1861 gcaccacagt ttttggcgca ccggtgtcat cacggttaag gttggcagca gggtaagcgg
     1921 ttaacacatg aagctgaata aatgttgtca tgagatagtc ctttattgat tttgttcgtt
     1981 atcggcgtcg ccagcctggt aatattccag cgcccagcgg atacggataa attcggttgg
     2041 ccgctgctgg cggcggtgat gattgagcag gtcgtcgctc tcctggcacc agcggaaaat
     2101 gccatctgcc agggagtcaa gattgacgga accgtttaat aatctgacgg cgcggcgtaa
     2161 ctggcgcagt aattcatccg gtgtttttac tgctgacagg cgagtaaaac gcccctttga
     2221 cattaccgcc gccagttgcg cggcaaaagg ctgccgttca tcaatggctt taacattggc
     2281 gctaactgcg gctatcagcg ccagtgcggt gatacgccac tcaggttcat cctgccactt
     2341 tatttgtctg ttcttcagaa acaggcgaaa tccatccgtc agacaaacat cattaaccgt
     2401 cgtactacgc cgcagactgg cacgttcgcc gcgtttctcc tgcaattcct cgtgccattt
     2461 gcgcagcgtg gctttgtgct cctcttttac aatactcatt cagcagcctc ctgctttttc
     2521 tccctggcgg ctttagcact ttgcttctcc gccgatgttg taaaatattt cttgcgcgcg
     2581 gtcatgatgc gttccaaatc aacgggctca taaggattgg tgaatacacg ctcgtcaaaa
     2641 tcctgacgcg cgaataacca aatttcctta ttccatttgc tgagtaattc atccgcatcc
     2701 tgaccttctt caatttggcg cactaacctc aggaagcgat gctgagtttt gttccagaag
     2761 tcgatatcca caaaactaaa atcacccctt gcaccttttg gatcggagaa ccatgcttct
     2821 ttcaatgcac tccgtaacag acttagaatc cgtgaagccg tttgcgcagc cagccgcagt
     2881 ttcggtatct ggccttcttt ttattgagca gcagcgggaa atgatgttcg taccaacagc
     2941 gcgctttcat gttgtcgaaa tcatagccaa atccccacag gcccactttt gcctgcttga
     3001 gactgctggc attaaagagt ttcaccacca gcgcgggaag ttccgtattg ttttctgact
     3061 tacccgtttc gataaggcct aaccagtcgc gccagattaa accgcccggt tgtggtttga
     3121 ccgagtaaaa ctcgccgccc tctttaagta gtacacggta aggcgttaag ggatgctgcc
     3181 acatggcata gttagcgccg taatttttgg tcgtcatcag gctcagaagc gcgtcgctct
     3241 gctcaccgca aatatcgcag ttgccgactg tggtggtatt aaaatcaata cgaatacggc
     3301 gaggcattcc ccagtacgcc tggagtttat tgacctgctc atcggttacc accgcaccgg
     3361 ccagttcgct gatgcgcgtc gggccaagcc aggggaaaac cagatcgtca aattttttgg
     3421 gtagcggtaa atcggcttca tcctgcggca tgacattgat ccacagtttg cgccacaagg
     3481 gagtttgttg attaccctga tattcctgta attcaatcag agtggtcatc ggcccaccgc
     3541 cgcgtaaacc ggtgcggtag cctttgccac ctgacggcgc attcaactgt agggagaaca
     3601 gagctaacgc agagcaatgt gggcacaggt attccgttac accacgcttg ataaagtggt
     3661 ctttattaaa cttcgttgtt tgagcgccgg gaatctcagg cagtagcgaa gcgaccggaa
     3721 ctttatctcc ggtgagcgcc tcgaaatcct gtataaatga aggggaatct ggcccaaact
     3781 ggaaagcatg ttctaatgac agcaatgctt cccgtagctt ttcagcttcc agtccgtctt
     3841 cccagatatc atcccaacga cgatgatctt ttggcgcgaa gctggtttgt agtagcccca
     3901 gcaaaaactg ccataccgca ccctggagat ctgcccgcgg cgcagaaata tcgacaacat
     3961 tttcatccgc cagatcgact ggcgccagtt tgcctgtcgt tccgtcttta aaacgaacgg
     4021 gcaaccacgg ggttgtcaga agtgaaaacg agttcatacg catcctctcg taaaaaagac
     4081 catcccaggt cggttgtcca tctttccatc cttttcggtg tatcaactat tcagagagtc
     4141 ctgtcgcaga catctcaacc aaccttgcca atcaatcctt ccgtagccga atacccacaa
     4201 ctttcgtcat cgttgactaa aataacgttt gccatctccg gatcttgtcg ctgttcaatg
     4261 caccacagcc tgaacgcctc tccctccagt aaagaaaact catcccgatg ttttttccac
     4321 cagcttcggc gcactctgac aacgctcatt tcccatgcgt gagcaccggt ggcataaggc
     4381 ttcaccacac cgtcaataca ggtagccagc cacagggaaa cagactcctc agccagacgt
     4441 gtcgacagtt tttctgggag gtaatcgttg atattggcgg catagccggg cctgaagttc
     4501 agcacaaact ttttagccat tgcgcgatca cagtaatatt tgcccacttg ctcctgctcg
     4561 ctgcgggcaa atccttccgg cattaccacg tcctcaccgt agacggattc aataagaagc
     4621 cgggctgcgt gtggcatctg aatagcacct tgctcacgca gcacacgctg cgtcagccag
     4681 attcgcccat gatcgggata gacaaatgca ctgttacgca tggcactgcc aaaccattcg
     4741 tcgccaggag agtcgtccca gacgggagcc agaatcagca attcaggggg ggaacgctcg
     4801 tcctttccgt cacgctttaa ctgaccatta atatcgcgga tatgccgctg taatcgtcct
     4861 gctcgctgaa tcagcaaatc aacaggggcc aggtcggaga tcatttcgtc caggtcacaa
     4921 tcgacactct gctctaatac ctgagtacaa ataaggactt ttccggcacg ctgtgagcta
     4981 tcttctttac caaagcgtgc cagtgtctcc gtttcaattc gctggcgatc gctaaaagca
     5041 aagcggctat gaaaaagtga aaggctggaa gcaggaatga cgccgcgggc aagcagctga
     5101 cgatgaacct taatagcgtc atcgacagaa ttccggatcc aggcgatgca tttcccctga
     5161 cttaccgccg attcgatacg cgcaatacac tcttgttcac tatgaagcca acccacgctg
     5221 acgctacgct caacgtcttt gcgcgtcgct acccggtgtg agttcacatc ggatttcgtg
     5281 acatgcgtca gccaggggta atcatccttt tccaggaacg gagcttcttg caggccctct
     5341 gtaccccgcg caaaggcagc gacgagtttg tcgcgctgct gttgagataa cgtggcagaa
     5401 agcaaaataa cgctgtttcc gccacgcgcc tgccgctcga tcaacccttc aagaatgcac
     5461 gacatgtaag cgtcacaggc gtggatctca tcagccagca ggattttgtt actcagcccc
     5521 agaagccgca gattattatg tttgaacggc atcaccgcca tcatcgcctg atccagcgtg
     5581 ccgacgccaa tttcagccag caacgccttc ttgttactgt cggcaaacca ggccgcacat
     5641 ccctgactga acgtttgttc atccggttct tctgacccga ctaaatcacc agaccagagt
     5701 gattcattaa agcggtccat taatgtgcgg gcactatgtg ccagcaccag gctggggcgg
     5761 gattctggcg aatagaaagc aagccaggtt ttgaccagcc gatcgtacat ggcattggcc
     5821 gttgccattg ttggcagacc aaaaaacagc ccctgagcct tccccgcagc tatcaacctg
     5881 tgcgccagga taagcgccgc ttctgtttta cctgcgccag tcacatcttc cagaataaat
     5941 aactgcggtc cttgctggct gatatccaga tccagcacct tttgctgtaa cggtgtcgga
     6001 ttctcaataa aaggaaacag cgtattaatt ccgttgaaag gcgcgacttt tgcttttaga
     6061 ggaaagacgg ttaaggcgtt ttgagcctga actaaagttt tctgccagta acctttaata
     6121 tccattgggt gtgcgacgcg tggaaaaaat cgcgttgacg aacccgtcca gtctgcgagc
     6181 acgacggttg cagagatata ccaggaaagg tgttttataa gttcaatgcc ctcatcatca
     6241 tcccagaatg cggggatctc tatgagcgga aacagtggtt tgatttcaag gagaaaatct
     6301 cgtgctgcag ctttgtcttc aggcagaaaa ttatccaatt catccatacg atcagggggc
     6361 caaccatgat gccctgtcgt cacgggcatc cacatttcta tcactcgcct aagtttgcgc
     6421 ggagagagcg aagatgaagg aagtaactcc tgacattcac ttaaataatg attccacaac
     6481 cagtagccca gcgttgaatg agagattttt tcgtaatttt ttctggcacc ttcctgaatc
     6541 ttgagttcag ggggcaggta aagttgttga aaagagcggg caaattttcc aatatcgtgc
     6601 cagcacagca accaggcgaa aaattgagcc gcctgttcct tgtcagaaat ccctaattga
     6661 cgaaaataat cagccagccc gaagcaattt cttttaacca ttaaatagcc catcgcggct
     6721 acatccagcg aatgccagca aagaaggtga tagtcgtcgc ccccgtcttt ctcgccgcgt
     6781 cgggtttttc cccagaaatc aaaaaaagtc acaatatttt tatccttcag taaacttaaa
     6841 ggatatttac gcatatatta aaaattatct gtgatatata tcattcaata aaatatttac
     6901 attatgaata atcaaataat tttcatccgt aatatatatg aataataaac ataggcataa
     6961 tattcacata aataatataa attaaaaaag ggtgcaggag accaaaccat cacaattcag
     7021 tacgcggggt tttacccccg cgtggtggct acttcttcga ttcgtaagcg agaactgcct
     7081 taaactccat accacgttct ctgatactca actcacacaa cgagtcgcct accatcagcg
     7141 tgaatcccag tactgtaaaa cacagcacta tgattgccac aagggcatat tttgtcagca
     7201 tgtcttgcct ccctgcaaag aagagactaa cattcgaatt gtttgggttc gaacgctggc
     7261 ctcaggttga tagaaatatc gcctggggct tttgtccatc tggaacctcg cgaatgctta
     7321 acgccagaca gcctcaagca cccgacgcca ttctatacct gataattctt ccagcggcgt
     7381 tcacgtttaa aatcaggaat actgctcgca aaataaaaac gcccccatca tttctgacag
     7441 aggcgtttaa tttatccggc aatatttacc cttcgtgcaa tccacattcc ctttttaagc
     7501 caaagaaacg tgtttcttct tccgacatgc caggttccca tttacgggtt gtatgggtat
     7561 caccgaccga taaatatcct tcatcccata atgggtgata tttcaggcca tgtttttgca
     7621 ggtactgata aatagttcgg ttatcccagt cgataatcgg cagcacttta aatacgccac
     7681 gctgaattgc cagcaccggt aaattggcgc gactgccgga ttgttcacgg cgcaggccag
     7741 caaaccaggt ttgcgcgtta agttctttca gagcccggtt catcggttcg actttgttga
     7801 tgtcattgta cttttcaatg ccttcaacgc cctgctccca cagttttccg tagcgtgctt
     7861 cctgccaggc tgcgctttcg gtagcacggt acactttcag gttgagcttg agtttgtccg
     7921 ttaactcgtc aataaagcgg taggtttccg ggaacaagta acccgtatcg gtgaggatca
     7981 ccggaatatc cgggcgaatt tgattcacca gatgcaggct caccgccgcc tgaatgccaa
     8041 agctggaaga aagtacatat tcaccgggca gattatccag cgcccaggct acgcggcctt
     8101 cagcgtccag tttttccagt tcggcgttag tttccgccag cgccagaata cgatctacct
     8161 tcggcagttc gttcagggcg tttagatcga gtttggacat agattcctca ctgtttgcct
     8221 tgcctgatgc gacgctcacg cgttcttatc aggcctacaa gaccgggctg atggttaatc
     8281 ccacaaatcg cgcgccggat cgagcaccgg gcgaatgatg cccgcacgca ccgtaaagtc
     8341 gccgaagcct tcacccgctt cgcgctcttt cgcccagcgc cctatcagtt catcaagcga
     8401 cgccaggatt tccggctcgg tgatgttttc tttatacatc cgtgggatac gtgtcccaat
     8461 gcggttgccg ccaagatgca ggttgtagcg acccggcgct ttacccacca ggcccacttc
     8521 cgccagcatc gcgcgaccac aaccgttcgg gcagcctgtt acacgcatca cgatatgctc
     8581 atcgctgaca ccatgtttcg ccattaaatt atcgatgttg tcgataaaag acggcaggaa
     8641 acgctctgct tccgccatcg ccagcgggca agtcgggaat gacacgcagg ccatcgagtt
     8701 ttcacgctgc ggcgtgacgg cattcattaa cccgctctct ttggcgatct tctcgatctt
     8761 cgctttctcg ctttccggta caccggtgat gatcagattc tggttcgccg taatgcggaa
     8821 atcgcctttg tggatcttcg cgatctccag caggccggtt ttcagcggac gccccggata
     8881 atcaaggatg cgaccatttt cgataaacag cgtcaggtgc cagttatcat caatgccctt
     8941 aacccagcca atacgatcgc ctcgtccggt gaactcatat ggacggatcg gttcaaattt
     9001 gatccccgcg cgacgctcca cttccgcttt aaacgtctca acccccacgc gctccagcgt
     9061 gtatttggtt ttggcatttt tacgatcggt tcggttaccc cagtcacgct gagttgtcac
     9121 gacggcttcg gccaccgcca gcgtatgctc cagcggcaga tagccaaact cgctcgccgt
     9181 gcgggcgtag gttttcttgt tgccgtgttc gatagaaagc ccaccgccca ccagcaggtt
     9241 gaagcctacc agcttgccgt tttcggcgat tgccacgaag ttcatgtcgt tggcatgcag
     9301 atcgatatcg ttctgcggcg ggatcactac cgtggttttg aacttacgcg gcaggtaggt
     9361 ctgaccaagg atcggttctt catcagtagt ggcgactttt tcctggtcga gccagatctc
     9421 cgcatacgcg cgggtacgag gcaacagatg ctcagaaatc ttcttcgccc actcgtacgc
     9481 ttccgcgtgc agctgcgact cgtaagggtt cgaggtgcag agtacgttac ggttcatgtc
     9541 gttagctgtc gccagcgcat caagaccgac cgagtgcagc atctggtgca ccggtttgac
     9601 gttctttttc agaatgccgt ggaactgaaa cgtctggcgg ttggtcaggc gaatgctgcc
     9661 atagatggtg ttttcaccgg caaatttgtc gatcgcctgc cactgtttag tggtaatcac
     9721 cccacccggc agacgacagc gaagcagcat cgcgtggcgc ggctccagct tctgttcagc
     9781 acgttcggca cggatgtcgc ggtcatcctg ctgatacatg ccgtggaagc ggatcagcag
     9841 gaagttgtcg cccttaaagc cgccggtcag accgtcgttt aaatcttccg caatggtgcc
     9901 gcgcaggtag ttgctttcaa gcttcatgcg ctcggcgtct gtcagttttc cttcgaccac
     9961 taaaggccct ggatgttttt cgctcattag tagacatctc gctgataacg gcgctctacg
    10021 cgcagctcac ttaaaaattc atccgccgct tcggtgtcca tgccaccaaa ttcggcaatc
    10081 acttccagaa gtgcctgctc aacgtctttc gccatgcgat tagcgtcgcc gcagacataa
    10141 atgtgggcac cgtcattgat ccagcgccac agctccgcgc cctgttcgcg cagtttgtct
    10201 tgtacataaa ctttttcttt ttggtcgcgc gaccaggcaa gatcgatacg cgtcagcacg
    10261 ccctctttga cgtagcgctg ccactccacc tggtagagga aatcttccgt aaagtgcggg
    10321 ttgccaaaga acagccagtt tttacccggc gcttcgtcgg cggcgcgttg ctgcataaag
    10381 gcacggaacg gcgcgatgcc ggtaccgggg ccaatcataa tcactggcgt ttgcgggtta
    10441 gcgggcaggc ggaaattgtc gttatgttcg ataaatacgc ggacttcgcc ctcttcttcc
    10501 acgcgatccg cgaggaagct ggaggcacca ccggcacggg cgcggccttc cacgtcgtaa
    10561 cgcaccacac caacggtgac gtgtacttcg ttctcgactt ccgcctgcga ggaggcgatg
    10621 gaatacaggc gcggggtcag cgggcgcagc aggttaatca gcgcttcggc atccagttgc
    10681 gccggagaga aacgcaccat gtcgacaatc ggcgtggtcg cggcgtaatg ctgtaacttc
    10741 gctttatcgc ccaccagcgg cagcagcgtt tcgctgcggg taagcgtggc atagttctca
    10801 acaatgttgg cggtgttgac ggtcagttcg aagtgccact gtagcgcttc gttcagaggc
    10861 aacgttttgc cctcgacggt gacaggttca tcgcctttca gccacagcag ttcgacaagt
    10921 tctttcacca gtgccgggtc gttctgatac cagacaccca gcgcgtcacc cggctggtaa
    10981 cgcaggcctg aatcgcctaa gtcaatttcg atatgacgaa cgtctttttc agagttacgc
    11041 ccggtaattt tctggttaac cgaaaggctc gccaccagcg gcgcgtcttt gctgtacggg
    11101 ctggtgtgga tttcatttac cgcgccagta gcgacggatt gcgaaggtgc cgcgacaggc
    11161 gcacgcgatt taagcgcatc aaccacgcgg gcgcgccact cgctggcagc agcctggtat
    11221 tcaacatcgg catcgacacg gtcgagcagg cgttcaccac ccagttccgc cagcttgctg
    11281 tcgaaatctt tcccggactg gcagaaaaat tcataagagc tatcgccgag gctaaacacg
    11341 gcaaacgcgg tgttttccag ctttggcgct tttttggaga acaggaactt atgcagcgcg
    11401 acggcttctt ccggcggttc cccttcccct tgcgttgacg tcactacgat gagcagtttt
    11461 tcgctggcga tttgtttgaa tttatagtcg cccgcgttca ccagcttaac gttcagtttt
    11521 gctgctaata aatcatcgcg taatgcttca gcaacccggc gcgcattgcc ggtttgcgag
    11581 gcggagataa tagttatacc cggcatttct gcggctggcg ctggcgtcgc tgcaagcgca
    11641 gcaggctgct gattgagtac gccccagaaa tagccagaaa cccaggcaag ctgggtaggc
    11701 gttaaatcgg tcgtggccgc ctgaaggcgt gccagttgct ccgggttcaa cggaagcaac
    11761 gcggaaggtg ggacctgtgt cgtcatgcgt cgttatgttc cagtaagcaa agctgtttct
    11821 gcgccctgtc agcgcccata aaacagaaga gaagataagg ttaacggggt gaacggtgtg
    11881 gattaaagac gggatagcga taactaataa ccaaatcgac taacctgttt tagcaatagt
    11941 ctttaacaac aaaatagatt aaccaaccca atgaaaaaca aatgaattta gccaatcatt
    12001 aagataaatc agcgattttg cgcaacaaag tcgttttaga taatgcgaaa aaacagcctt
    12061 tccggtactc tacggcggtt ttatcgtcct gtagagaaat tatgatgtcc accacgttat
    12121 ttaaagattt caccttcgaa gccgctcacc gcttaccaca cgtcccggaa gggcataaat
    12181 gtggtcgcct gcacgggcat tcctttatgg tgcgactgga aattaccggg gaagtcgatc
    12241 cgcatacggg ctggattatc gatttcgctg aactgaaagc ggcgtttaag ccaacttatg
    12301 agcgcctcga tcaccattat ctcaatgata ttccaggtct ggaaaaccca accagcgaag
    12361 ttttagcaaa atggatttgg gatcaggtta aacccgttgt gccgctgtta agtgcggtga
    12421 tggtaaaaga aacctgcacc gcagggtgta tctatcgcgg cgaatgataa gagtgtgtcg
    12481 gcggtcaatt tcccttaagt aacgctatgt tagggtgttg tgttctggat atctggggca
    12541 tgacatggaa gacgactgcg acattattat tattggtgcc ggtattgcag gcaccgcttg
    12601 cgcgttacgc tgcgcgcgag cgggtttatc cgttttgtta ctggaacgcg ctgaaatccc
    12661 cggcagcaaa aatctttccg gcgggcggtt atatacccat gcactcgcgg aactcctccc
    12721 gcaatttcat ctgaccgcgc ctcttgaacg acgcatcact cacgaaagcc tttccctgtt
    12781 aacgcccgat ggcgtaacga cgttttccag cttacagccc ggcggtgaat cctggagtgt
    12841 attacgtgca cggttcgatc cgtggctggt tgccgaagcc gaaaaagaag gtgtcgaatg
    12901 catccccgga gcgacggtgg atgcactgta tgaagaaaac ggcagagtct gtggcgttat
    12961 ttgtggtgac gatattctcc gcgcccgtta tgtggtgctg gcagaaggtg ccaacagcgt
    13021 cctggctgaa cgtcacgggt tagtgactcg tcctgctggc gaagcgatgg cgttggggat
    13081 caaagaagtg ctgtcgctgg aaacatccgc tattgaagaa cgttttcatc tggagaataa
    13141 cgaaggcgca gcgttgctgt tcagcggcgg aatctgtgat gacttacccg gcggcgcatt
    13201 tctttatact aatcaacaaa cgctctcgtt agggattgtt tgcccgctct cttcccttac
    13261 gcaaagtcgt gttccggcaa gcgagctgct gactcgcttt aaagcgcatc cggcagtgcg
    13321 cccgcttatc aaaaacacgg aatcactgga gtatggtgcg catctggagc cagaaggtgg
    13381 cttgcacagt atgccggtgc aatacgccgg taacggctgg ctgctggtgg gcgatgcgtt
    13441 gcgcagttgc gtcaataccg gaatttccgt gcgcggcatg gatatggcgc tgactggcgc
    13501 acaggcggcg gcacaaacgc taataagcgc ctgccagcac cgcgagccgc aaaatctgtt
    13561 tgcgctttat catcacaacg tagagcgcag cctgctatgg gatgttctac aacgttatca
    13621 gcatgttccg gcgcttttgc aacgccctgg ctggtatcgg gcgtggcctg cgttaatgca
    13681 ggatatttcc cgcgatttat gggatcaggg tgataaacct gttccaccgc tgcgccagtt
    13741 attctggcgt catttacgtc gtcatggcct gtggcatctg gcgggcgatg ttatcaggag
    13801 tctgcgatgt ctgtagccca taatctctgg cgcgttgctg atgcgccgca cattgttccg
    13861 gctgactccg ttgagcgtca gatggtacaa cggttgatta acgcctgtcc ggcaggtctt
    13921 ttttcgctca caccggaagg tgacttacgc gttgattatc acggctgcct ggagtgtggc
    13981 acctgccgtt tgctgtgcga cgaatccaca ctacaacagt ggcgctatcc gccttccgga
    14041 ttcggcatca cctaccgctt tggataaata ataaggataa tttatgcccc tcttacacct
    14101 actccgccag aatccggtga ttgctgccgt taaagataat gccagcctgc aactggctat
    14161 cgattctgaa tgccagttta tttccgtgtt gtacggcaat atctgcacca tcagtaatat
    14221 cgttaaaaag attaagaacg ccgggaaata tgcttttatt catgttgatt tgctggaagg
    14281 cgcgtcaaat aaagaagtcg ttattcagtt tttgaaactg gtgaccgagg cggacggcat
    14341 tatcagtacc aaagcctcaa tgttgaaagc ggcaagagcg gaaggttttt tctgtattca
    14401 tcgcctgttt attgttgatt caatttcgtt tcacaacatt gataagcaag ttgcgcaatc
    14461 gaatccggat tgtattgaga tcctgccagg cggtatgccc aaagtgctgg gctgggtgac
    14521 agagaaaatc cgccaaccgc tgattgccgg tgggctggtg tgcgatgaag aagatgcgcg
    14581 taatgcgatt aacgcgggtg tcgtggcgct ttccaccacg aataccgggg tctggacgtt
    14641 agcgaaaaaa ttactttgac gggataatcg taaccaattg aatttggttt gatttgtagg
    14701 ccgcacgcca catccgacat tcagcgcctg atgcgacgct tgacgcgtct tatcaggcct
    14761 acaagtcccc aggccagaac cgtaggcagg atcatccggc aacacgttat tggcaatctg
    14821 cgtgaatgtt tgtcaccagt gcctccagca ccactttcca gtcatcaacc acgccaacat
    14881 ctgcctgtga aaataccgca gcgctggcgt catgattaat cgccaccaca aacttgctat
    14941 tgcgtacgcc cgccattaac gctgctgcgc cagaagcgcc aacaacaata cacacctcag
    15001 gtgccagcag atgtccggaa ataccaatca ctttttcagc atcaacgcca ccgttcatca
    15061 ctcgcgcccg actgtagccc acttcggctc ccagcttttc tgccagcata gcgatttcct
    15121 ggttatctgc ctctccgccc tgccccacca ccagcacccg tctggcctca gctaatgggt
    15181 cgcgagtaac atttttcaga tcctctgtgc taaccagcca gtccggaagc gcacccggga
    15241 caatgttcag ttgttgcatt ccagagggca acgtggcgtt tttcgctgct cccgcctgac
    15301 gcgccaggga aaggcatagc gggcgttttt ctgtttgcag cgtcgccgtt agcgcatttc
    15361 cccagtggga tttgcgcacg cttacggttg gaatatctaa cgaagtcacc tggcagatgc
    15421 ttgcaccatg taaacgccag gccagccggg tcgacagttc atcgccaaac gttcccggcg
    15481 gaaagagaac aacgtccgca ggcgttcgct gccactgttc caccaacgca tccagcacct
    15541 gttccgccac tacaggctga ggttcaatct gccagtgcgc cagggtacaa ccgctgaaat
    15601 cctgcgccgc cagccagctt gctattgcgg cattctcctg atttattgtc acaattgcga
    15661 tattcatggc tgcatccttt ggcgtaaata gtcctgccat agcttttgag ctttttctgc
    15721 taccgtttgc ccatcaatca gcgtcgctcc gcgtcgttgc tctgctcttg ccagttgcag
    15781 acactgcatc gctggcattt cggcagcaac agttttacgg ataatttctg ctttccccgc
    15841 agccattcgc tggcgcattc ccggtactgg caaggccact tcaccgcact gacgcacggc
    15901 aatcaccgca ggcaagcgaa cccggcaaca acgcaaccca tgttcagtac gctgttcgag
    15961 ggtgataaac agcgcgtcga gagtgaaacg ctccacctgg gtgaagcagg gccagcccag
    16021 catttccgcc agcaaaaatg gcgtttgccc attctgccct tcgctgcttt ggcagccggt
    16081 aattatcaga tccagcggat tctgatgctg ccattcggca atatggcgag cgacaaattc
    16141 cggcgcaaag cgcagatctg ccgccgtctc cagcaataca gcttcctcaa acccaagagc
    16201 catgagatag cgcagccagt gcaatgcccg ttcatccccc atgctcaacg cggttaaaga
    16261 catcggcgta ccgttttttc tctgcgccaa cagcagcgcg gcagcggcct gttcatcagc
    16321 accgagtaaa cttcgcagta gcgaaatatc cggtccgctt ttaccctgag ccgccgcctg
    16381 ccactctttt tccgccagca ttccggcatc cggttcggct ttaaacgcta acagaatgtt
    16441 cattcgcacc tcctacattt cccgccgcca ccagcgggag ggctttagtt tctggtgccc
    16501 atagccaggt cacaacaaaa tcaaccaaca acactgtcgc caggagcaat aaggtgactt
    16561 gcattcccca ctgcgccagc acccacggca gcaggccagt acttaccgcc gcgccaagtc
    16621 gactcatggc ggtggcaaaa ccgacgccca gcgagcgaat gtcagtagga aaactttccg
    16681 caggcaaaat gcccaccaga ttactgactg ccgaaatggt ggtgctgaag agaacaaaaa
    16741 gcagcagcgt taatgaactg ccggaaggca aacaggccat cactaccagc gttgccgcca
    16801 gcagcaaaaa acttcccagc aaaaatttgc gatgtgccag caggtgcgtc agaactaatc
    16861 ccagcagcgc gcccacaatt aacaacgcat taagcatcag gctggcagtc agcgcatctt
    16921 ccagaccaat agtctgggcg atagttggca gccaggtata aatcacaaac catgggatta
    16981 cgaggcagac aaagaagacg ctgttaaacg ccgtgcgccg ccagtaacgc gaagagaaca
    17041 aggttttgat gtgtttatgg gtcgccgtta ccacttcatc gcccagtaaa acatggggac
    17101 caaaatagcg atgcacgata gcgtgagctt ctgcaaaacg cccctggcgc agtagccagc
    17161 gtggtgattc tggcgttccc cagcgtaata acgtaatcaa caacgcgggc agagctgccg
    17221 atgccagtag ccagcgccag gcctccgggt tttcggaaat aaagtgatgt ccggcaatac
    17281 ttgccagcac atagcctacg gtccacacca cgctgaatgc gcccagcaaa ataccgcgat
    17341 ggcggcgcgg ggaaaattca gccagcaagg tgtgacctac tgaataatcg cctcccagac
    17401 caatgccaat caaaatgcgc agtccaataa gatgctctgg cgtggtggca aaaaattgta
    17461 aaaacgaagc aagcgtaatc agcaaaaagc tgaaggtgaa gattttttgc cgaccaatat
    17521 ggtcggagat ccacccaaga accaggctgc caaggaacaa accaaggagc gccgagccgc
    17581 cgatcattcc cgccataaac ggcgtcagtt gcatggcggg cgtaagctga ataatggcgt
    17641 aaccaatgac gccgagaaca taaccgtcgg tcaggtgtgc gccgaaagtg agcgcagcaa
    17701 tgcggcagtg aaaacggtta agcggtaaat catccattcg caccggtgaa gtgttcatct
    17761 ttaccccttc attgtccggt aataagaacg gcttctcgcc tgagaagccg aaaaggcaac
    17821 gccccgtcat cgcggaacgc tgcctgacac attatttttc aatcggatag atagtacccg
    17881 tattcataat gccattagga tcgaactgct ttttcagccc ttccagcaac gcccacgcgc
    17941 tgccgtgttc cagtttgctc cagtgaacgc gatgtttacc gataccgtgg tggtgcacca
    18001 tcgaaccgcc gaggcgaatg gtttcttcac agatgatctt gttgagcgga ttgtggtact
    18061 tgtcgatttc ctcttccggc ttacagtcaa cgacgttgta atcgtagacg aagtacatgt
    18121 tggtgccgtt ctgatagcta tgagaggaat gaccgcccag catggtgatg tcgtcggcgt
    18181 gcgggaactc agtacgaata cggttaataa cgctttcgta gatttcgtgg atgcagctcc
    18241 agcagccgga cacttcagtg gtaaagccca tgttgccggt tttgaggatc tgcacacgtt
    18301 cggcagccac tttatccggt ccccagttca ggttgttaaa ccaggtttcg atcagcttgc
    18361 tgtccacgcg ctggcattgc gggtagcggg caacgatttc cgcaatccct tcgcccgtcg
    18421 ccttcgcaat gcgagggtta ccttcagcca taaagatcag cacgcatttt ccgtctgcaa
    18481 aatgggtgaa gtgttgggtg ccatcttcag cgtcatacaa acgagcgatc gacggacgat
    18541 acccttccac catgacttca cgcaggatgt tgaagtcggt tttcatgtct tccaggatat
    18601 agccgtagaa gaggttgttt tccggggtga atttaaagat tttcactgtt acttcagtga
    18661 tatagcaaag tgcaccttcg ttaccgatga tgatgtggcg aatgtccggg ccagccgcgc
    18721 ggcgtggcac gtttttaatg cgtgtgacgg tgccatcagc caatactgct tccagaccaa
    18781 cgaccatatc ttcgattgcg ccgtagagtg tggagaactg cccgatactg cgggttgcta
    18841 ccaagccgcc catctgcgcc agcggctttg actgcggaga atgccccgtg gtgtaacctt
    18901 tttcacgcaa cgcattttcc agcacctcca gcggaacgcc acattgcgcc gtcgcctgca
    18961 tattctcaat atcaatatta atgatttgat tcatggcgga gccgtcaagc accaccgagt
    19021 tttccacaac ggtttccagc ccaccttcag tggcggaagc gccggtacgc ggcacaccgt
    19081 taattttgtg cgcattcata aaattcagca cacgggatac ttgctctgtg gaaccgagtt
    19141 ttacgaccgc tgccggaatc ggcaaagtat aaatgccatg aatatccgga aatttacgaa
    19201 aacggtcaat actgttcttc tttaatactg tttcatcggt aattacgcga tctgcaccaa
    19261 caatttcctt tagttggtcg acaatcgctg cgcgagataa agacatagta attccttcct
    19321 gataataaaa atggaataat cattaataag agaaaacaga aaccaccgtt atggattagc
    19381 gcactaaata accaccattg accactaata aatgcccatt gacataattt gaagccggac
    19441 ttgcgaggaa tacagctgcg cccattaaat cctgagtatc gccccaacgg tttgccggaa
    19501 tatgatcaag aacgcgctga ttggtttctg gattactgcg tgtcgccagc gtaatatctg
    19561 ttgcataata gccaggagca ataccattta cctgaatatt atattgacct agttcatcac
    19621 aataagcttt ggtgaatccg gcaagagcat gtttagtggc agaatatgca ggtgaccatt
    19681 gtccacctaa gtaagagaac aatgaacaga tattaataat tttgccgctt ttctgcggga
    19741 tcataatttt tgcagcttca tagcttaact cgaatgcggc ggtcaggttc acatcaatca
    19801 tcggatccca gtcggcacga ccgaagtcca gcaccttatt cagcttacaa ataccggcat
    19861 tgtttaccag aatatcaact gtaccgaaac gctcacagca agcagcgata atcttctgcg
    19921 gcgcgccttc tgcggtgata tccacctgca tgaagtccac ctcaacaccc tgtttttcaa
    19981 tcatttcctt tgtttcgccg ttatctttca atcatttcct ttgtttcgcc gttatctttg
    20041 acgaaactag gaataaagat atttgcgcca gctttggcca acgccatggc aaatgcctgg
    20101 cctaaaccgc tattcccacc ggtaacaatt gcggttttac ctttcagtga gaaaaaatcc
    20161 attgagaacg cattgagaga ttcgattgac atagttagct ccaatttccg taaggcaaaa
    20221 aaaagaaagg taacttcccc cgcgagaggg aagttacctt tctcattatc tctggtaact
    20281 ggagataatt tttagcataa cggcataact gctaatgtga tcgtaatcac agtgtgatat
    20341 tcgtattgtc aaatagtttt aaaaagcaga agcgtgatta ctctcacaca acacctctgc
    20401 tttccatgtt atttcttatt gcaattacta gagacctgag aaaagtaatc cacctgaaag
    20461 ggcggcttac ttttctcttt tttttgtcta ttacaaatgg gaatacgata tgcaacacaa
    20521 ctcatatcgc cgttggataa ccctcgcgat aattagtttt agcggcggcg ttagttttga
    20581 cctggcttat ttacgttata tttttcaaat tcccatggcg aaatttatgg gattcagcaa
    20641 taccgagata ggtttaataa tgagtacctt tggtattgcg gctattattc tttatgcccc
    20701 cagcggcgtt attgccgata aattttcaca ccgcaaaatg attacttccg cgatgatcat
    20761 taccggatta ctgggtctgt taatggcaac gtatccaccg ctgtgggtaa tgctctgtat
    20821 tcaggtcgcc tttgcgataa cgacgatttt aatgctgtgg tcggtgtcga ttaaagccgc
    20881 ctcgttgctg ggcgatcata gtgagcaagg gaaaattatg ggctggatgg aagggctgcg
    20941 cggcgtcggt gtaatgtcgc tggcggtgtt taccatgtgg gtcttttctc gctttgcacc
    21001 ggatgacagc gccagcctga aaacagtcat tatcatctac agtgtggttt acatcttgtt
    21061 ggggattctg tgctggtttt ttgttagcga taacaacaac ctgcgcagtg ccaataacga
    21121 agaaaaacag tcattccagc ttagcgacat ccttgccgtt ttgcgtatca gcaccacctg
    21181 gtattgcagc atggtgattt ttggtgtctt cacaatctac gccattctga gttactccac
    21241 caactatctg accgaaatgt acggcatgtc gctggtggca gcgagctaca tggggattgt
    21301 gatcaacaaa atcttccgcg cgctgtgcgg cccacttgga ggcattatca ccacctacag
    21361 taaagtgaaa tcccctaccc gcgtgattca aatcctttcc atcatcggtt tgctggcgtt
    21421 gacagccctg ctcgtcacga actctaaccc ccaatcggtc gcgatgggga ttggcctgat
    21481 tttactgctg ggattcacct gttacgcctc acgcgggctg tactgggcct gccccggcga
    21541 agcgagaaca ccgtcttaca ttatgggcac cacggtaggt atttgttcgg tgattggatt
    21601 cctgccggat gtcttcgttt acccgattat cggccactgg caagacaccc tgcccgctgc
    21661 agaagcctac cgcaatatgt ggctgatggg catggcggcg ctcggcatgg tgattgtctt
    21721 tacctttttg ctgttccaaa aaattcgtac tgctgatagc gcccccgcaa tagctagcag
    21781 caagtaagcc aggaaaatta caggagaaaa atcatgtcga aaaaatacat catagggatt
    21841 gatggcggaa gtcagagcac aaaagtggtg atgtacgatc tggaaggtaa tgtggtttgc
    21901 gaaggtaaag gcttattaca gccgatgcac acgcctgatg ccgatactgc agaacatcct
    21961 gacgacgatt tatgggcatc attatgtttt gcaggtcacg atttgatgag ccagtttgcc
    22021 gggaataaag aagatattgt tggcatcggc ctgggctcca tccgttgctg ccgtgcgtta
    22081 ttgaaagccg acggcacgcc agctgcgccg ttgattagct ggcaggatgc acgcgttaca
    22141 cgcccttacg aacatacgaa tcctgacgtg gcgtatgtaa cctctttttc gggttatctg
    22201 acgcatcgct taaccggcga atttaaagat aatatcgcca actattttgg tcagtggccg
    22261 gtggattata agacctgggc atggagcgaa gatgccgcag tcatggagaa gtttaatatc
    22321 ccccgtcata tgctgtttga tgtgcaaatg ccaggcactg ttcttgggca tatcacacct
    22381 caagccgcgc tggcgacgca tttcccggca ggactgccgg ttgtttgtac caccagtgat
    22441 aaaccggtag aagctctggg agccggatta ctggatgatg aaactgcggt aatttctctt
    22501 ggcacttaca tcgcattgat gatgaacggc aaagccttgc cgaaagatcc ggtggcgtac
    22561 tggccgatta tgtcttctat tccgcaaaca ttgctgtatg aaggttacgg tattcgcaaa
    22621 ggcatgtgga cggtgagctg gctgcgcgac atgttaggcg agtcgttaat tcaggatgcc
    22681 agggcgcagg atctttcacc ggaagattta ctcaacaaga aagcctctag tgtgccacct
    22741 ggctgtaatg ggctgatgac ggtgctggac tggctgacca atccgtggga accgtacaaa
    22801 cgcgggatta tgatcggctt tgattccagc atggattacg catggatata tcgttcgata
    22861 ttggaaagcg tggcgctgac gctgaagaac aattacgaca atatgtgtaa tgaaatgaat
    22921 cactttgcga aacatgtaat cattactggc ggcggttcga acaggatctg tttatgcaga
    22981 tttttgccga cgtgttcaac cttccggcac gccgtaacgc cattaacggt tgtgcaagcc
    23041 tgggggcagc gattaataca ggggtaggtc tggggctata cccggattac gcaacggctg
    23101 tcg
//
LOCUS       DAJVQG010000059        22847 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJVQG010000059 DAJVQG010000000
VERSION     DAJVQG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22847
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.059"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..1354
                     /gene="cscA"
                     /locus_tag="OQM83_002026"
     CDS             <1..1354
                     /gene="cscA"
                     /locus_tag="OQM83_002026"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCS1520293.1"
                     /translation="FHLAPPAGWMNDPNGLIWFNDRYHAFYQHHPMSEHWGPMHWGHA
                     TSDDMIHWQHEPIALAPGDDNDKDGCFSGSAVDDNGVLSLIYTGHVWLDCAGNDDAIR
                     EVQCLATSRDGIHFEKQGVILTPPEGIMHFRDPKVWREADTWWMVVGAKDPGNTGQIL
                     LYRGSSLREWTFDRVLAHADAGESYMWECPDFFSLGDQHYLMFSPQGMNAEGYSYRNR
                     FQSGVIPGMWSPGRLFAQSGHFTELDNGHDFYAPQSFLAKDGRRIVIGWMDMWESPMP
                     SKREGWAGCMTLARELSESNGKLLQRPVHEAESLRQQHQSVSPRTISNKYVLQENAQA
                     VEIQLQWALKNSDAEHYGLQLGTGMRLYIDNQSERLVLWRYYPHENLDGYRSIPLPQR
                     DTLALRIFIDTSSVEVFINDGEAVMSSRIYPQPEERELSLYASHGVAVLQHGALWLLG
                     "
     gene            complement(1362..2420)
                     /locus_tag="OQM83_002027"
     CDS             complement(1362..2420)
                     /locus_tag="OQM83_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1520294.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2588..2806
                     /locus_tag="OQM83_002028"
                     /pseudo
     CDS             2588..2806
                     /locus_tag="OQM83_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            2824..4152
                     /gene="dsdA"
                     /locus_tag="OQM83_002029"
     CDS             2824..4152
                     /gene="dsdA"
                     /locus_tag="OQM83_002029"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCS1520295.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4260..5798)
                     /gene="emrY"
                     /locus_tag="OQM83_002030"
     CDS             complement(4260..5798)
                     /gene="emrY"
                     /locus_tag="OQM83_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCS1520296.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(5798..6961)
                     /gene="emrK"
                     /locus_tag="OQM83_002031"
     CDS             complement(5798..6961)
                     /gene="emrK"
                     /locus_tag="OQM83_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCS1520297.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7377..7991
                     /gene="evgA"
                     /locus_tag="OQM83_002032"
     CDS             7377..7991
                     /gene="evgA"
                     /locus_tag="OQM83_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCS1520298.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            7996..11589
                     /gene="evgS"
                     /locus_tag="OQM83_002033"
     CDS             7996..11589
                     /gene="evgS"
                     /locus_tag="OQM83_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCS1520299.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11645..12790)
                     /gene="yfdE"
                     /locus_tag="OQM83_002034"
     CDS             complement(11645..12790)
                     /gene="yfdE"
                     /locus_tag="OQM83_002034"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCS1520300.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(12864..13808)
                     /gene="yfdV"
                     /locus_tag="OQM83_002035"
     CDS             complement(12864..13808)
                     /gene="yfdV"
                     /locus_tag="OQM83_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCS1520301.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(13878..15572)
                     /gene="oxc"
                     /locus_tag="OQM83_002036"
     CDS             complement(13878..15572)
                     /gene="oxc"
                     /locus_tag="OQM83_002036"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCS1520302.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15626..16876)
                     /gene="frc"
                     /locus_tag="OQM83_002037"
     CDS             complement(15626..16876)
                     /gene="frc"
                     /locus_tag="OQM83_002037"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCS1520303.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17389..18012)
                     /locus_tag="OQM83_002038"
     CDS             complement(17389..18012)
                     /locus_tag="OQM83_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCS1520304.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18309..18584
                     /gene="ypdI"
                     /locus_tag="OQM83_002039"
     CDS             18309..18584
                     /gene="ypdI"
                     /locus_tag="OQM83_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HCS1520305.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18661..18903)
                     /locus_tag="OQM83_002040"
     CDS             complement(18661..18903)
                     /locus_tag="OQM83_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCS1520306.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19256..20176
                     /gene="lpxP"
                     /locus_tag="OQM83_002041"
     CDS             19256..20176
                     /gene="lpxP"
                     /locus_tag="OQM83_002041"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCS1520307.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20532..20603
                     /gene="ypdK"
                     /locus_tag="OQM83_002042"
     CDS             20532..20603
                     /gene="ypdK"
                     /locus_tag="OQM83_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCS1520308.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20668..21906)
                     /gene="alaC"
                     /locus_tag="OQM83_002043"
     CDS             complement(20668..21906)
                     /gene="alaC"
                     /locus_tag="OQM83_002043"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCS1520309.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22283..>22847
                     /gene="ypdA"
                     /locus_tag="OQM83_002044"
     CDS             22283..>22847
                     /gene="ypdA"
                     /locus_tag="OQM83_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCS1520310.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVSILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIV"
BASE COUNT         6384 a         5356 c         4997 g         6110 t
ORIGIN      
        1 ttttcacctc gcgccacctg ccggatggat gaacgatcca aacggcctga tctggtttaa
       61 cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg gaccaatgca
      121 ctggggacat gccaccagcg acgatatgat ccactggcag catgagccta ttgcgctagc
      181 gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg atgacaatgg
      241 tgtcctctca cttatctaca ccggacacgt ctggctcgat tgtgcaggta atgacgatgc
      301 aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg agaaacaggg
      361 tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag tgtggcgtga
      421 agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg ggcagatcct
      481 gctttatcgc ggcagttcat tgcgtgaatg gaccttcgat cgcgtactgg cccacgctga
      541 tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg atcagcatta
      601 tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa atcgctttca
      661 aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat ccgggcattt
      721 tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag cgaaggatgg
      781 tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct caaaacgtga
      841 aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg gcaaacttct
      901 acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg tctctccccg
      961 cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga ttcagttgca
     1021 gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca ctggaatgcg
     1081 gctgtatatt gataaccaat ctgagcgact tgttttgtgg cggtattacc cacacgagaa
     1141 tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc taaggatatt
     1201 tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga tgagtagtcg
     1261 aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg gagtggctgt
     1321 gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga acaacggatc
     1381 aacagcggac aagggatccg cgtcactctt cccccttcac gaccttcaat aatatgcaat
     1441 gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg cggtaaaaac
     1501 agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg cgggatacgt
     1561 acccccttcg ccaggagaac ctgataagcg acaaaggctg cacgatcgtt accacatatc
     1621 agaacatcaa aatctggttt gcccgatttg aagtgggcat tgagtaaact tgcgagatcg
     1681 gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag atctcgtcca
     1741 gcatcacgcc aggcctgctc aaatccctgc cgacgatacc ctgttgccaa cgcactttcc
     1801 ggtagccaga agcataacgg ttgacgatag cccgccgcga gcaaatgctg tgttgattca
     1861 tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc cgccacacag
     1921 ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg tcgcagcccc
     1981 attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc cgcgcgggca
     2041 gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc gctggcggtt
     2101 tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc ctgcgccagc
     2161 acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga aagatcagga
     2221 acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg acgcacagat
     2281 tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag gcgtgcgaca
     2341 tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta gcagaaacag
     2401 acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata aaaaataaca
     2461 ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag taaccatata
     2521 ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa tataaatatg
     2581 gatcattgtg ctgccgctgt atcccgacat cagcccggaa attattgcga ttgctatcgg
     2641 ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctag tgaagcaata
     2701 ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat ttatcgcttc
     2761 agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc aaagagacgt
     2821 actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt aaaggatctg
     2881 gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc tgaaggttta
     2941 ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt atcccgtttt
     3001 gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat tattgaatca
     3061 gaactggttg ccattccagc tatgcaaaaa cggctggaaa aagaatatca gcaaccgatc
     3121 agcgggcaac tgttactgaa aaaagatagc catttgccca tttccggctc cataaaagca
     3181 cgcggcggga tttatgaagt cctggcacac gcagaaaaac tggctctgga agcggggttg
     3241 ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca gttctttagc
     3301 caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg cattatgagc
     3361 gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc atggaaaaaa
     3421 gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta tggtgttgcc
     3481 gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat tgatgacgaa
     3541 aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa agcgcaattt
     3601 gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct gccgtgtggt
     3661 gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg cgatcatgtt
     3721 cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt ccatacagga
     3781 ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc agcggatggc
     3841 cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct gctggatggc
     3901 ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc gcaggaagaa
     3961 ggtattcgtc ttgaaccttc ggcactggcg ggtatggccg gacctcagcg cgtatgtgca
     4021 tcagtaagtt accaacagat gcacggtttc agcgcagaac aactgcgtaa taccactcat
     4081 ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca atatctggca
     4141 aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt gagcgatgct
     4201 gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc cttttatcat
     4261 cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac cgtaagtaag
     4321 ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag tgactgctgg
     4381 gttatttcat tattaatttc attaagaact cctgaaagcg aaccataata tttatccata
     4441 atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt caactgacta
     4501 tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc aactgatcct
     4561 gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt atctggcaag
     4621 cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa tccctgaaaa
     4681 aactgcggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac agaacgccag
     4741 taatagcaaa ccgcatacat caaaaaacta aatgtcacta acaaccgcat gtctattttg
     4801 ttgccataac gtcctatcaa aggtgaaatt aatagtggca tgatgccgat gggcgcataa
     4861 gcaagtccgg cccatatcgc attatacccc atcgtttcct ggagtaactg cggcataagg
     4921 acgatcgctc cagagtaaaa taaatacgcg catgtgatac tcacaatacc aatggtgaag
     4981 ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga ctcccaaatg
     5041 actaaagaga tcagagaaat aactgatact actgttaata ttattattgt actcgagttg
     5101 aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac accgagcact
     5161 aacagggtca gtcctggtag attcattttg accggtgaag tctcagtttc tcttccttta
     5221 agtaaggtta agcataatgt caggacgata atccccatag ggacattgat taaaaatatc
     5281 caaccccagc taaagttatc acaaatataa ccgcccaata tcggcccaca tatcggagcg
     5341 ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg cggataattc
     5401 cttaataata aactctgtga cagtggaatt aacggccccg ccattaaccc ctgaacgact
     5461 ctaaaaaata tcagcacatc aagattggtc gataggctac acattaatga agacagcgaa
     5521 aaaaaggtta ctgaaagtaa aaataatctt aattcgccta ttctttgtgc caacctgcca
     5581 gtaacaggga tcgcaatggc atttgctaca ccaaacgagg tgataaccca ggtgccttcg
     5641 tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga aatagtggaa
     5701 tccaacattt gcataaatgt cgctaatgac aatgcaatag tgacgcacca taacgtccca
     5761 ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca ttatgcgaaa
     5821 taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc ttactggtat
     5881 aagccggcat ggaggtcacg gttgaagcca gctcaggcat ctcagcaatg tcttcgtcct
     5941 tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc atgagttctt
     6001 ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag ttccctgtcg
     6061 cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc cctgtcaccc
     6121 gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact gattgaccaa
     6181 tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt tgacgtgccg
     6241 gtacgacagc cattaacgat tgtccgggac tcactgtttc gccgacctga acacttcgct
     6301 gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcgcc aaccaggctt
     6361 ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt gtgttcatta
     6421 ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct ttgctactta
     6481 ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc ttcgctaaag
     6541 gcacacgacg gttataatct tctaaggatt gttgatactg aatacgtgct gaagcgactt
     6601 cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca atatttgcca
     6661 gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt gaaactaaaa
     6721 tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca ctacctgaga
     6781 cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta ctaatcatgt
     6841 cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat aaaactatca
     6901 ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta atctgttcca
     6961 ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga gtattcattc
     7021 ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga ggcctaattt
     7081 aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat acaattctta
     7141 cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa tcatcggtat
     7201 aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg tatacttgtc
     7261 gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga gatgacgcct
     7321 tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa taatctatga
     7381 acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat ttattgatca
     7441 aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt cagcgggtgg
     7501 aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt aacggtatcc
     7561 tggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc gtctccgcta
     7621 aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt ttcgtgagta
     7681 aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat ggctactgct
     7741 atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag caaaaactcg
     7801 actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc aaggataata
     7861 atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacttat aaaagtcgcc
     7921 tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgca caacgtaaca
     7981 aaatcggcta accacatgaa gtttttaccc tatatttttc ttctctgttg tggtctttgg
     8041 tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag tagtaacaac
     8101 cgtgtcatac ttgatccagt acgtctgagc aacaaggaat tacgttggtt agcgagcaaa
     8161 aaaaatcttg tgattgcagt acataagtcc caaacggcta cgttgttgca taccgattcg
     8221 cagcaacgag ttcgtggtat taatgctgat tatttaaatc ttttaaaaag aacgttaaat
     8281 atcaaattaa cactccggga atacgccgat catcaaaaag caatggacgc gctggaagaa
     8341 ggtgaagtcg atatagtgtt atcacattta gtagcttcgc cgcctcttaa tgatgacatt
     8401 gctgcaacca aaccactgat aattaccttt ccggcgctgg taaccaccct tcacgattca
     8461 atgcgaccgc ttacctcatc aaaaccagta aatattgctc gagtagcaaa ttatccccct
     8521 gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac aaatttatat
     8581 caggcattag catccgtctc agccggacag aatgattact ttattggtag taacatcatt
     8641 accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa atattataac
     8701 tcaccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcgttct taatgaagta
     8761 ctcaatcgat ttgttgatgc tttgacaaat gaagttcgct atgaagtatc acaaaattgg
     8821 cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga acatgaaaaa
     8881 caatggatta agcagcatcc cgatttaaag gtgctggaaa atccttactc ccctccctat
     8941 tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggatattct taatattatt
     9001 actttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat ccatgctgga
     9061 acacagctga atcccggcgg atgggatata ttacccggtg ctatttatag tgaagataga
     9121 gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt tgtcatgcaa
     9181 aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat tccatattat
     9241 tatgagcttc attcgcaatt aaaagagatg tatccggagg tagagtggat aaaagtcgat
     9301 aacgccagcg ctgcatttca caaggtcaag gaaggcgaac ttgatgctct ggtcgcgaca
     9361 cagttaaatt cacgttacat gatcgaccat tactatccta atgaacttta tcattttctt
     9421 attcccggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga accggaactt
     9481 aaggatatta ttaataaagc actgaatgca atccccccaa gcgaagttct gcgcctgacc
     9541 gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctcta tagcgagcaa
     9601 ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt atggggattt
     9661 tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga aaaccaaata
     9721 tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt aaactggcaa
     9781 ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga ttactacaaa
     9841 aatgcaatgt taccattaga aaacagtgac tcccccttta aagatgtttt ttctaatgcg
     9901 catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt ttttgaaatt
     9961 gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa tctgccagcc
    10021 agcgatcatg ctgtttatat ttgtggttgg caagatatta cagagacgcg tgatttaatt
    10081 catgcactcg aagtagagag aaataaagcg atcaatgcaa ctgtcgcaaa aagtcagttt
    10141 ctggcaacaa tgagtcacga aataagaaca ccaataagct ccattatggg cttcctggaa
    10201 cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc acttgcctac
    10261 tccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga caaaattgaa
    10321 tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt ccagaacact
    10381 tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaatttg cagcagtaca
    10441 tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt cttatcaaat
    10501 ttactgagca atgctctcaa atttaccacc gagggggcag taaaaattac gacctccctg
    10561 gttcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc tggaagtgga
    10621 ttatcgcagg aagaacaaca acaactcttt aaacgctaca gccaaacaag tgcaggtcgc
    10681 cagcaaacag gatctggctt aggcttaatg atctgcaaag aattaattaa aaatatgcag
    10741 ggcgatttgt cattagaaag tcatccaggc ataggaacaa catttacgat cacaatcccg
    10801 gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaccc catcacacta
    10861 cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct attactcaaa
    10921 cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt gcaagcgcta
    10981 cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat gccgaatatg
    11041 gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc catctggggg
    11101 cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg catgaactta
    11161 tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca gttacaccag
    11221 gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa aaataatacg
    11281 gcgaacgatc tacaactgat gcaggagatt ctcatgactt tccagcatga aacgcataaa
    11341 gatctacccg ctgcgtttca agcactagaa gctggcgata acagaacttt ccatcagtgt
    11401 attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat taatattagc
    11461 catcagttag aaataacacc tgtttcagat gacagtaagc ctgaaattct tcagttgctg
    11521 aactctgtaa aagaacacat tgcagagctg gaccaggaga ttgctgtttt ctgtcagaaa
    11581 aatgactaaa tagcggctcc cacaatgttc taatgtggga gctatttacc agcacatctt
    11641 acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc gttgccgctc
    11701 ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc ggcatcatta
    11761 ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta atggcctcag
    11821 ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct aaccaaactt
    11881 cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc tggttttgta
    11941 cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc tccagtgcct
    12001 ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc ttatcctgag
    12061 tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt tgtggtgact
    12121 tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg gcatcaaaca
    12181 tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt gcactcacta
    12241 ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca acacgcactg
    12301 gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga atgatggtat
    12361 cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat gaagcatata
    12421 tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt tccattgtac
    12481 ctgggcgaaa attctcagct aatacatcag cttgtttgag catatttata aatatacttt
    12541 tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta ataaaactgt
    12601 aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca ccatgacctg
    12661 gtggctcaac tttaattacc cttgcgccca tattacaaag aagttgagtt ccgaaaggtc
    12721 cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct ttgctttcat
    12781 tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact tcggcattta
    12841 tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat ccacaaggga
    12901 gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt acgggtgtag
    12961 acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc gcctgccagt
    13021 accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag aagcagcgcc
    13081 agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact gaactcgaat
    13141 ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt agccttcgca
    13201 atcagattaa aggtcgggtc ccatgctgcc ggaattttta ccccaaccag caccaggatc
    13261 gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa tgcgctcaga
    13321 ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag accaatagga
    13381 atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt tgatacggaa
    13441 tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg tgaacccgca
    13501 attaatgcac atacagcggc ttctgcatgg gtacgtttaa aaaatttgta gcaaccaaac
    13561 caagagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag acgagtgtcc
    13621 gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc aggaagagcg
    13681 tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt ttctcgtctg
    13741 ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc aataaaaaat
    13801 gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga gttggcaaga
    13861 ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt ggtaatatgg
    13921 ccactttcag ttcctgcagc agggtcgatg accacattaa taatggtcgg tttgcgcgac
    13981 tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt atagccaacg
    14041 ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc cgttggtgat
    14101 ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc attattaaaa
    14161 ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat cccactgaaa
    14221 ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac gctagcaccg
    14281 atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag acgacgacgt
    14341 ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc ttcattaact
    14401 aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc attaaagtaa
    14461 tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt ttgctgcttg
    14521 tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg tttcagttct
    14581 gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc aatggggcgg
    14641 ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc tgccgcccat
    14701 ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat gacaacgtca
    14761 gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt atcttcaagg
    14821 atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat aaattcacga
    14881 agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa tggccgttca
    14941 gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa taatgctggc
    15001 gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat cgtcgcggcc
    15061 aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac agagacccgg
    15121 atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa tgctgctttg
    15181 gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg ctgtaggtcg
    15241 acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt taccgttgcg
    15301 ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag gcagatcccc
    15361 ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg ctcatgacga
    15421 aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt cacaggaata
    15481 cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac gatgatatgc
    15541 ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat ttttataaat
    15601 tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt gcagcaattt
    15661 catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct aatagcggcg
    15721 cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg gtcagatatt
    15781 ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa gagggatcaa
    15841 gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga gtcaaatagg
    15901 ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca aaaatatcga
    15961 aaatatgtgg ctgtcgggca tgggctgtac tgtatgccga atcggtaatc cattctggtt
    16021 tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa taaatatagg
    16081 cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga ccgccaccac
    16141 ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac tgcgggtatt
    16201 cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg cggcaaaggt
    16261 tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt ttttcgcgat
    16321 gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg tcacccaacg
    16381 ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc gcgccacccg
    16441 ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac tcatcaaacc
    16501 ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc caggtgaagc
    16561 ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct tcgcggatca
    16621 gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata gaacgtttgt
    16681 tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc agttggtgac
    16741 gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca aaccaggcca
    16801 gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact ttaattcctt
    16861 gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa tttagcgttt
    16921 atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc ggcactcgat
    16981 atttttaaat tcctgtatca agtactgaaa attatttcat cgccacttcc aattcctttc
    17041 tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atatttattc gtggtaatca
    17101 gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc ggtgatttac
    17161 aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata ataaatgatt
    17221 tgtgatgaca ctggattatt gcggtttact ttgaggaaaa taaaatgcgt gtttatagaa
    17281 ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat aaaaaattgt
    17341 cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt agttcacaaa
    17401 cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca ggtttgcctc
    17461 gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac gagtctgttt
    17521 aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa gctcttccat
    17581 tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg cggcttcttt
    17641 ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga caatatattg
    17701 gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt cggtactgtc
    17761 atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg gatcgccatg
    17821 aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt gttcagagat
    17881 acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg ctaccggtgc
    17941 attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg tagccataat
    18001 taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc atcatattgg
    18061 gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta tcttacaatt
    18121 gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc ttcctgtcgc
    18181 agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg taaatattga
    18241 ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca ttttcaggat
    18301 aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc tctattatgg
    18361 catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc tataaagaga
    18421 ctgaaaaaac atcagcgatg acaaccacac actctacaaa acttcagcca tcacaggcga
    18481 ttttgtttaa gatgagagaa gatgcgccac cattaaacct cacagaagaa atcacgccta
    18541 cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg tcacatggca
    18601 ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca tcctgaccca
    18661 ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg gggcaagaaa
    18721 cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga agtaataaat
    18781 gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca cgttcagtac
    18841 ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc ataaataaat
    18901 catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt aatagtaaga
    18961 caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg ttaaatgcaa
    19021 cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag ctgaaagcag
    19081 tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg tcctgactag
    19141 tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat tgagtgtacc
    19201 gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg taactatgtt
    19261 tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca catggtttgg
    19321 gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct ttctcggcac
    19381 gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg cccgtaaaaa
    19441 cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga ttgccgaaaa
    19501 ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct ggcccgacag
    19561 tcgcgtacgt aaatggtttg atgttgaagg gttggataac cctaaacgcg cacaaatgca
    19621 aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg gcggccgcgt
    19681 gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc agctgatgga
    19741 atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca gaaataatct
    19801 gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc ccgatcagga
    19861 ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg tcgccacaac
    19921 caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg taacgatggt
    19981 aagaaaagcg gattacagcg gatatcgttt gtttatcacc ccagagatgg aaggctaccc
    20041 gacagatgaa aatcaagccg ctgcctatat gaacaagatt atcgagaaag agatcatgcg
    20101 cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg tgggagaatc
    20161 gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg ccataataac
    20221 ctcatggttt ttatggcttt tttcttaagg ttttttcctt cgctgaatat ctaattatta
    20281 ccgtcagaaa ttaaactgtc gccgatatga gacacttgta agttactaaa agtaaataag
    20341 aaaatgcctc ttgtctcgtt tcagttttag gcgtacatta acgccgtctt gagcagttta
    20401 gctcagaatt ctggggtgat gcccacggta aaatacaggg cttttaaggt cgctttgccc
    20461 ggtgtcaact cactgtatca ggtgtaatga agtcattcag gcgtaacagt aattacgcgg
    20521 agagatgtaa agtgaaatat ttctttatgg gcatttcttt tatggtcatc gtttgggccg
    20581 gtacttttgc cctgatgatc taaaagcaaa ctgtgcaatg aaaaacagga gccatcaggc
    20641 tcctgtttgc atttctgacg acggtgttta ttccgcgttt tcgtgaatat gtttgctgct
    20701 ggcgggtagt aaaccgtcgg cacggaacat cgctttaatc cctctgatgg cctgacgaat
    20761 acggtcgcgg ttttcaatca gtgcaaagcg aacatgcgta tcaccatagt cgccaaagcc
    20821 aatccccggc gagacacaaa ccttcgcttc gttaaggagc ttcttggcaa attccagcga
    20881 tcccatggcc gcatatggtt ccgggatttt cgcccagaca tacatcgaag ccttcggcat
    20941 ttcgaccatc cagcccgctt catgcagccc tttaaccaat acatcacggc ggcgtttgta
    21001 ctgttcggta atgtcgcgca cgcactgttg atcgccctcg agtgccgcaa tcgccgccac
    21061 ctgcaacggc gtaaaagtgc cgtaatcgtg atagcttttg attcgcgcca gggcactgac
    21121 cagcgttttg ttgccgacca taaagccgat acgccagccc gccatgttgt agcttttcga
    21181 cagcgtaaag aactcgaccg ccacatcgcg tgcacccggc acctgcataa ttgacggcgc
    21241 tttccagcca tcgtagacga tatcggcata ggccaggtca tggaccacca gcacatcgta
    21301 gcgtttcgcc agcgccacaa ccttttcgaa gaactccagt tccacgcatt gcgcggttgg
    21361 gttcgacggg aagccgagga tcatcatctt cggtttcgga taactttcac gaatggcgcg
    21421 ctccagttcg ttgaagaaat cgacaccttc caccagcggc actgagcgta cctgcgcccc
    21481 ggcaatcacc gcgccataaa tatggatcgg gtaacttgga ttcggcacca gcaccgtgtc
    21541 accatgatcc agcgtcgcca gcatcagatg cgccaggccc tctttcgaac caatagtgac
    21601 gatggcttct gattccgggt cgatttcaac gtcgtagcga tcctgatacc agcgggaaat
    21661 ggcgcgacgt aaccgcggaa tgccgcgtga agtggagtaa ccatgcgtgt ccgggcgctg
    21721 ggccacagta cataattttt cgacgatatg cggcggagtc gcaccgtccg ggttacccat
    21781 gctgaaatcg ataatatctt cgccgcgccg acgcgcagcc attttcagtt cagcggtgat
    21841 gttaaaaacg tagggcggga gacgatcaat gcgcgtaaag cgacgttcag ggcgagtgtc
    21901 agccataatt tcctcagatt aacgttagcg cccggaccgt ccgagcgacg ctgccacaat
    21961 ggtggctgtt ttgaaaatag cctgattaat ttctgtctgt ccagcgtttt agaacatttt
    22021 tatccatggc tttaaatgag gaactccact tcaaaaacag gaaatttttc tacgctaaca
    22081 agaaaattac caccaatttt attaacatta taatttcaat aaatttacca gccagaagtg
    22141 cattatcaag agatacattg ctaatacacc atttatcttt caatcccctt ttaaatttat
    22201 ggtttttcct caaaaacggc ctgctggtca tcagccttca ggttacctat catagaggtt
    22261 taatccttat tcagagtcac ccgtgcacga aatattcaac atgctgctgg cggtcttcga
    22321 tcgggcagcg ttaatgctta tctgcctgtt ctttctcatc cgtatccgcc tgtttcgcga
    22381 actgttgcac aagtcggcgc actccccaaa agaattgctc gccgttaccg ccattttctc
    22441 gctgttcgcc ctgttcagca cctggtccgg cgttcccgta gaaggctcgc tggtgaacgt
    22501 acgtattatc gcggtgatgt ccgacgggat tctgtttggc ccgtgggtag gcatcattac
    22561 cggcgtgatt gcgggtattc accggtattt aattgatatc ggcggcgtga cggcgatccc
    22621 ctgctttatc accagcattc tggcgggttg tatatcaggc tggataaacc tgaaaatccc
    22681 caaagcacag cgctggcgcg tcagtattct cggcggcatg ttgtgtgaga cgttgaccat
    22741 gattctggta attgtctggg caccaactac cgcgttgggg atcgatatcg tctctaaaat
    22801 cggcattcca atgatcctcg gtagcgtctg tatcggcttt attgtgc
//
LOCUS       DAJVQG010000060        22580 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJVQG010000060 DAJVQG010000000
VERSION     DAJVQG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22580
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.060"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            233..1339
                     /gene="ompC"
                     /locus_tag="OQM83_002045"
     CDS             233..1339
                     /gene="ompC"
                     /locus_tag="OQM83_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCS1520311.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1451..2506
                     /gene="apbE"
                     /locus_tag="OQM83_002046"
     CDS             1451..2506
                     /gene="apbE"
                     /locus_tag="OQM83_002046"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCS1520312.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2580..3644
                     /gene="ada"
                     /locus_tag="OQM83_002047"
     CDS             2580..3644
                     /gene="ada"
                     /locus_tag="OQM83_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCS1520313.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3644..4294
                     /gene="alkB"
                     /locus_tag="OQM83_002048"
     CDS             3644..4294
                     /gene="alkB"
                     /locus_tag="OQM83_002048"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCS1520314.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4370..6013
                     /gene="yojI"
                     /locus_tag="OQM83_002049"
     CDS             4370..6013
                     /gene="yojI"
                     /locus_tag="OQM83_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCS1520315.1"
                     /translation="MELLVLVWRQYRWPFISVMELSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6231..7877
                     /gene="mqo"
                     /locus_tag="OQM83_002050"
     CDS             6231..7877
                     /gene="mqo"
                     /locus_tag="OQM83_002050"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCS1520316.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8186..8674)
                     /gene="eco"
                     /locus_tag="OQM83_002051"
     CDS             complement(8186..8674)
                     /gene="eco"
                     /locus_tag="OQM83_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCS1520317.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            9082..9576
                     /gene="napF"
                     /locus_tag="OQM83_002052"
     CDS             9082..9576
                     /gene="napF"
                     /locus_tag="OQM83_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCS1520318.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            9566..9829
                     /gene="napD"
                     /locus_tag="OQM83_002053"
     CDS             9566..9829
                     /gene="napD"
                     /locus_tag="OQM83_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCS1520319.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            9826..12312
                     /gene="napA"
                     /locus_tag="OQM83_002054"
     CDS             9826..12312
                     /gene="napA"
                     /locus_tag="OQM83_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCS1520320.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            12319..13014
                     /gene="napG"
                     /locus_tag="OQM83_002055"
     CDS             12319..13014
                     /gene="napG"
                     /locus_tag="OQM83_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCS1520321.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            13001..13864
                     /gene="napH"
                     /locus_tag="OQM83_002056"
     CDS             13001..13864
                     /gene="napH"
                     /locus_tag="OQM83_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCS1520322.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            13861..14310
                     /gene="napB"
                     /locus_tag="OQM83_002057"
     CDS             13861..14310
                     /gene="napB"
                     /locus_tag="OQM83_002057"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCS1520323.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            14320..14922
                     /gene="napC"
                     /locus_tag="OQM83_002058"
     CDS             14320..14922
                     /gene="napC"
                     /locus_tag="OQM83_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCS1520324.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            14941..15558
                     /gene="ccmA"
                     /locus_tag="OQM83_002059"
     CDS             14941..15558
                     /gene="ccmA"
                     /locus_tag="OQM83_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCS1520325.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            15555..16217
                     /gene="ccmB"
                     /locus_tag="OQM83_002060"
     CDS             15555..16217
                     /gene="ccmB"
                     /locus_tag="OQM83_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCS1520326.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            16259..16996
                     /gene="ccmC"
                     /locus_tag="OQM83_002061"
     CDS             16259..16996
                     /gene="ccmC"
                     /locus_tag="OQM83_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCS1520327.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            16993..17202
                     /gene="ccmD"
                     /locus_tag="OQM83_002062"
     CDS             16993..17202
                     /gene="ccmD"
                     /locus_tag="OQM83_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCS1520328.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            17199..17678
                     /gene="ccmE"
                     /locus_tag="OQM83_002063"
     CDS             17199..17678
                     /gene="ccmE"
                     /locus_tag="OQM83_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCS1520329.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            17675..19618
                     /gene="ccmF"
                     /locus_tag="OQM83_002064"
     CDS             17675..19618
                     /gene="ccmF"
                     /locus_tag="OQM83_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCS1520330.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSIDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            19615..20172
                     /gene="dsbE"
                     /locus_tag="OQM83_002065"
     CDS             19615..20172
                     /gene="dsbE"
                     /locus_tag="OQM83_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCS1520331.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            20169..21221
                     /gene="ccmH"
                     /locus_tag="OQM83_002066"
     CDS             20169..21221
                     /gene="ccmH"
                     /locus_tag="OQM83_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCS1520332.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(21256..21903)
                     /gene="narP"
                     /locus_tag="OQM83_002067"
     CDS             complement(21256..21903)
                     /gene="narP"
                     /locus_tag="OQM83_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCS1520333.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            22223..>22580
                     /locus_tag="OQM83_002068"
     CDS             22223..>22580
                     /locus_tag="OQM83_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1520334.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVGLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAVNQTTLNNGVL"
BASE COUNT         5056 a         5668 c         6422 g         5434 t
ORIGIN      
        1 atgttaggtg cttatttcgc cattccgcaa taatcttaaa aagttccctt gcatttacat
       61 tttgaaacat ctatagcgat aaatgaaaca tcttaaaagt tttagtatca tattcgtgtt
      121 ggattattct gcatttttgg ggagaatgga cttgccgact gattaatgag ggttaatcag
      181 tatgcagtgg cataaaaaag caaataaagg catataacag agggttaata acatgaaagt
      241 taaagtactg tccctcctgg tcccagctct gctggtagca ggcgcagcaa acgctgctga
      301 agtttacaac aaagacggca acaaattaga tctgtacggt aaagtagacg gcctgcacta
      361 tttctctgac aacaagtcag aagacggcga ccagacctat gtacgtcttg gtttcaaagg
      421 cgtaactcag gttactgacc agctgaccgg ttacggccag tgggaatatc agatccaggg
      481 caatacctct gaagacaaca aagaaaactc ctggacccgt gtggcattcg caggtctgaa
      541 attccaggat gtgggttctt tcgactacgg tcgtaactac ggcgttgttt acgacgtaac
      601 ttcttggacc gacgtactgc cagaattcgg tggcgacacc tacggttctg acaacttcat
      661 gcagcagcgt ggtaacggct tcgcgaccta ccgtaacact gacttcttcg gtctggttga
      721 cggcctgaac tttgctgttc agtaccaggg taaaaacggt agcgtaagcg gcgaaggcat
      781 gaccaacaac ggtcgtggtg ctctgcgtca gaacggcgac ggcgttggcg gatctatcac
      841 ttatgattac gaaggcttcg gtatcggtgg tgcgatctcc agctccaaac gtactgatga
      901 tcagaacagc ccgctgtaca tcggtaacgg cgaccgtgct gaaacctaca ccggtggtct
      961 gaaatacgac gctaacaaca tctacctggc tgctcagtac acccagacct ataacgcaac
     1021 tcgcgtaggt tccctgggtt gggcgaacaa agcacagaac tttgaagctg ttgctcagta
     1081 ccagttcgac ttcggtctgc gtccgtctgt agcatacctg cagtctaaag gtaaaaacct
     1141 gggtaccatc gctggtcgta actacgacga cgaagatatc ctgaaatatg ttgatgttgg
     1201 tgctacctac tacttcaaca aaaacatgtc cacctacgtt gactacaaaa tcaacctgct
     1261 ggacgacaac cagttcactc gtgacgctgg catcaacact gataacatcg tagctctggg
     1321 tctggtttac cagttctaat ctcgattgat atcgaacaaa gggcctgcgg gccctttttt
     1381 cattgttttc agcgtacaaa ctcatttttt tggtgtactc ttgcgaccgt tcgcatgagg
     1441 ataatcacgt atggaaataa gctttacccg cgtggcactg ctggctgccg cgctcttctt
     1501 tgttggttgc gatcaaaaac cacaacccgc caaaacccac gctactgaag ttaccgttct
     1561 agaaggcaaa accatgggta ccttctggcg tgccagcatc ccgggcattg atgccaaacg
     1621 cagtgccgaa cttaaagaaa agattcagac ccagctggac gccgacgatc agctgctttc
     1681 gacctataaa aaagattccg cgctgatgcg ctttaacgat tcgcaaagtt tatcgccgtg
     1741 gccggtaagt gaagcgatgg ccgatatcgt caccacctcg ctgcgcattg gcacgaagac
     1801 cgatggcgcg atggatataa ccgtcgggcc gctggtgaat ctgtggggct ttggcccgga
     1861 acaacagtcg gttcaaattc cgagccagga acagatcgat gcgatgaaag ccaaaaccgg
     1921 cttacagcac ctgacggtca ttaatcagtc gcatcagcaa tatctgcaaa aagacctgcc
     1981 ggatttaaat gtcgatctct ccaccgtcgg tgaaggttat gcggcggatc acctggcacg
     2041 cttgatggag caggaaggga tttcccgcta tctggtgtcg gtgggcggcg cgctgaacag
     2101 ccgtggtatg aacggtgaag gcctgccgtg gcgggtagcg attcaaaaac caaccgataa
     2161 agaaaacgcg gttcaggctg tggtggatat caacggtcat gggattagca cctctggcag
     2221 ctaccgtaac tattacgaac tggacggcaa acgtctttcc catgttatcg atccgcaaac
     2281 cgggcgtccc atcgaacaca atctggtatc cgtgacggtg attgctccga cggcgctgga
     2341 agccgatgcc tgggatactg gcttgatggt actcgggccg gagaaagcca aagaggttgt
     2401 tcgccgggaa gggctggcgg tttatatgat caccaaagaa ggcgatagct ttaaaacctg
     2461 gatgtcacca cagtttaaaa gcttccttat cagcgaaaaa aattaaagcg caagattgtt
     2521 ggtttttgcg tgatggtgac cgggcagact aaaggctatc cttaaccagg gagctgatta
     2581 tgaaaaacgc cacatgctta actgacgatc aacgctggca atctgtctta gcccgcgacc
     2641 cgaatgccga cggcgaattc gttttcgccg tgcgcaccac gggtatcttt tgccgtccgt
     2701 cttgccgcgc cagacatgcc ttgcgggaaa atgtctcctt ctacgcaaat gccagcgagg
     2761 cactcgccgc cggatttcgc ccctgcaaac gttgtcagcc agacaaagcc aatccccggc
     2821 aacatcggtt ggataaaatc acccacgcgt gtcgactgct ggaacaggaa acgcctgtaa
     2881 cgctggaagc cttagccgac caggtggcga tgagtccatt tcatctacat cggttgttta
     2941 aagcgactac cggaatgacg cctaaagcct ggcaacaggc ctggcgcgct cgccgtttgc
     3001 gcgaatcgct ggcgaaaggg gagagcgtga cgacgtctat tcttaacgcc ggattccccg
     3061 acagcagcag ttactaccgc aaagccgatg aaacgctggg catgacggct aaacaattcc
     3121 gtcacggtgg cgaaaatctg gcggtgcgtt acgcgctggc tgattgtgag ctgggtcgtt
     3181 gcctggtggc agaaagcgag cgggggattt gcgcgatatt gctgggcgat gatgacgcga
     3241 cattaatcag cgagttgcag cagatgtttc ccgctgccga caacgcgcct gccgatctga
     3301 cgtttcagca acatgtgcgt gaagtgatcg ccagcctcaa tcaacgcgat acgccgctga
     3361 cgttaccgct ggacattcgc ggcactgctt ttcagcaaca agtctggcag gcactgcgca
     3421 cgataccttg tggtgaaacc gtcagttatc agcaactggc taacgccatc ggcaaaccga
     3481 aagcggtacg ggccgttgcc agcgcctgtg ccgccaacaa gctggctatc gtaataccct
     3541 gtcatcgagt ggtccgtggt gatggcacac tttccggtta ccgctggggc gtgtcgcgta
     3601 aagcgcaact gctgcgccgc gaagctgaaa atgaggagag gtaatgttgg atctgtttgc
     3661 cgatgctgaa ccgtggcaag agccactggc ggctggtgcg gtaattttac ggcgttttgc
     3721 ttttaacgct gcggagcaac tgatccgcga tattaatgac gttgccagcc agtcgccgtt
     3781 tcgccagatg gtcacccccg ggggatatac catgtcggtg gcgatgacca actgtgggca
     3841 tctgggctgg acgacccatc ggcaaggtta tctctattcg cccattgatc cgcaaacaaa
     3901 taaaccgtgg cccgccatgc cacaaagctt tcatgattta tgtcaacgtg cggctacggc
     3961 ggcgggctat ccagatttcc agccagatgc ttgtcttatc aaccgctacg ctcctggcgc
     4021 gaaactgtcg ctgcatcagg ataaagacga accggatctg cgcgcgccga ttgtttctgt
     4081 ttctcttggc ttacccgcga tttttcaatt tggtggcctg aaacgtaatg atccgctcaa
     4141 acgtttgttg ttggaacatg gcgatgtggt ggtatggggc ggtgaatcgc ggctgtttta
     4201 tcacggcatt caaccgctga aagcggggtt tcatccactc accggcgact gccgttacaa
     4261 cctgacattc cgtcaggcag gtaaaaaaga ataaaaataa gaattattat tgctgtgcgc
     4321 gcgaagatgt ttaaactgcg ggctgtaatt cattgtccgg gttttctgca tggaacttct
     4381 tgtacttgtc tggcggcagt atcgctggcc atttatcagt gtgatggagc taagcctcgc
     4441 cagtgcggct ttaggcattg gcttaattgc ttttatcaat cagcgcctta tcgaaacggc
     4501 ggataccagt ctgctggtgt tgccggagtt tctgggatta ttgctgctgt tgatggcaat
     4561 cactctcgga tcgcaactgg cgctcaccac tttggggcat cacttcgttt accgactgcg
     4621 tagtgaattt atcaagcgga ttctcgatac ccacgtcgag cgcattgaac aactcggtag
     4681 cgcctcgttg ctggcgggat taaccagcga tgtgcgcaat atcaccattg cttttgtgcg
     4741 tctgccggaa ctggtgcagg ggatcattct cactatcggt tcagcggcgt atctgtggat
     4801 gctgtcgggc aaaatgttgc tggtaacggc tatctggatg gcggtcacca tctggggcgg
     4861 ttttgtgctg gtggcgcggg tgtacaaaca tatggcgacc ctgcgtgaaa ccgaagataa
     4921 gctgtacacg gattttcaaa ccgtactcga agggcgcaaa gagctgactc tgaaccgtga
     4981 acgcgccgag tatgtgttta acaacctcta cattcctgat gcgcaagagt atcgccatca
     5041 tattatccgc gcagacacct tccatcttag tgccgtgaac tggtcaaaca tcatgatgct
     5101 gggcgctatc ggcctggtgt tctggatggc gaacagcctc ggttgggctg ataccaacgt
     5161 tgctgcgacc tattcgttga cgctgttatt cctgcgtacg ccgctgcttt cggcggttgg
     5221 cgcattgccg acgctgctga cggcgcaggt ggcgtttaac aagctgaaca aattcgcgct
     5281 cgcgcctttc aaagcagagt ttccgcgccc gcaggcgttt cccaactggc aaacgctgga
     5341 gctgcgtaat gtgacgttta gctatcagga taacgcgttt tccgttgggc cgattaacct
     5401 caccattaaa cgtggcgagc tgctgtttct gattggtggc aacggtagtg gaaaatcgac
     5461 gctggcgatg ttgctgacgg gcttgtacca gccacaaagc ggcgaaatct tgctggatgg
     5521 caaacctgtc agcggcgaac aaccggaaga ttatcgcaaa ctgttttcgg cagtgtttac
     5581 cgatgtctgg ctgtttgatc aactgctggg gccggagggg caacccgcta acccgcaact
     5641 ggttgagaag tggctggcgc agctgaaaat ggctcataag cttgagttaa gcaacgggcg
     5701 tattgttaac ctgaagttat caaaagggca gaaaaaacgc gtggcgctgt tgctggcgct
     5761 ggcagaagaa cgcgatatta tcctgctgga tgaatgggcg gcggatcagg atccacactt
     5821 ccgtcgtgag ttttatcagg tgttgctgcc gctgatgcag gagatgggta aaactatttt
     5881 cgccatcagt catgatgatc attactttat ccacgccgac cgcctgctgg aaatgcgcaa
     5941 tgggcaactt agcgagctga cgggcgaaga gcgcgatgcc gcttcgcgtg atgccgttgc
     6001 ccggacggca taatattacg acgctgacct ggcggactgc tgccgccagg tcaatattct
     6061 aaaaatccct tcatttctaa taaccctata attaatcaac gaaattataa tgtttctaaa
     6121 attagaatat aatttataaa cattatttaa atgttgttac tttagtgtta ccgttgatgc
     6181 cgcgcaaatc tcacctgcaa taaagcgact aaaagtaagg cattaacaag atgaaaaaag
     6241 tgactgccat gctcttctcg atggccgtgg ggcttaatgc cgtttcgatg gcggcaaaag
     6301 cgaaagcgtc cgaggagcag gaaactgatg tactgttgat tggcggcggc attatgagcg
     6361 ccacgttggg gacctattta cgcgagctgg agcctgaatg gtcgatgacc atggtggagc
     6421 gcctggaggg tgtcgcgcag gagagttcga acggctggaa caacgccgga accgggcatt
     6481 ctgcactgat ggaactgaac tacaccccgc aaaacgccga tggcagtatc agtattgaaa
     6541 aagcagtcgc cattaacgaa gcatttcaga tttcccgcca gttctgggcg caccaggtcg
     6601 agcgcggcgt gctgcgtact ccgcgttcat ttatcaatac cgttccgcat atgagctttg
     6661 tctggggcga ggataacgtt aatttcctgc gcgcccgtta cgccgcgttg caacaaagct
     6721 cgctgtttcg cggtatgcgt tactctgaag atcacgcgca gatcaaagag tgggcaccgt
     6781 tagtgatgga agggcgcgat ccgcaacaga aagtggcagc cacgcgtacg gaaattggta
     6841 ccgatgtgaa ctacggcgag atcacccgcc agttaattgc ttccttgcag aagaaatcta
     6901 acttctcgct gcaactcagc agcgaagtcc gcgccctaaa gcgtaatgac gataacacct
     6961 ggaccgttac cgttgccgat ctgaaaaatg gcactgcaca gaacattcgt gccaaatttg
     7021 tctttatcgg cgcgggcggt gcggcgctga agctgttaca ggaatcgggg attccggaag
     7081 cgaaagacta cgccggtttc ccggtgggcg gacagttcct tgtttcggaa aacccggacg
     7141 tggttaatca ccatctggcg aaggtttacg gtaaagcatc cgttggcgca ccaccgatgt
     7201 cggttccgca tatcgatacc cgcgttctgg acggtaaacg cgtagtgctg tttgggccat
     7261 ttgccacctt ctcaaccaaa ttcctcaaaa acggttcatt gtgggatctg atgagttcca
     7321 ccaccacctc taacgtgatg ccgatgatgc acgtcgggct ggataatttc gatctggtga
     7381 aatacttggt gagtcaggtg atgttgagtg aagaggatcg ttttgaagcg ttgaaagagt
     7441 actatccgca ggcgaaaaaa gaggactggc gtttgtggca agcggggcag cgtgtgcaga
     7501 ttatcaagcg tgatgccgat aaaggtggtg tactgcgtct gggtactgaa gtggtcagtg
     7561 accagcaagg gaccattgcc gcgctgctgg gggcatcgcc aggggcgtca accgctgcgc
     7621 cgattatgtt ggatctgctg gaaaaagtat ttggcgatcg cgtttccagc ccgcaatggc
     7681 aggctacgtt gaaagcgatc gttccatctt atggacgcaa gctgaacggt gatgtagcgg
     7741 caacagaacg cgagttgcag tacaccagcg aagtgctggg cctgaaatac gacaagccgc
     7801 aggcagcaga tagtacgccg aaaccgcagt tgaaaccgca acccgttcaa aaagaagtgg
     7861 cggatattgc gttgtaatga tgtgccacat ccggcatggt atgccggatg tggcgtaacc
     7921 gccttatccg acctacgggg tggagcactt cacagcggtg taggcctgat aagacgcgtc
     7981 agcgtcgcat caggcaaccg gctcgggcgt tagatgtcgg atgcggcgta accgccttat
     8041 ccaacctacg gggtggagca cttcacagcg gtgtaggcct gataagacgc gccagcgtcg
     8101 catcaggcaa ccggcttggg cgttagatgt cggatgcggc gtgaccgcct tatccgacct
     8161 acggggtgac gcacttcaca gcagtttagc gaactaccgc gttgtcaatt ttctcttccg
     8221 ccttccagac gcggtacttc acatctacat tgtctggcgt atacaccacg atcggcagct
     8281 tgctgttgta acgcagcatt ccagcatcgc ccagatacgc ggtgacaaat ttcttctctt
     8341 tcttgccatc cgggcaggcc atcatcgtgg aaaccggcga gctaacttta tcaaagacat
     8401 aataatcata gccccagcct tccagcgttt tgttttccag cttcccgccg agacgatgca
     8461 aattgcaatc gacttccagc gtctgaccgg ttaacagttc tactttcagg gtagattcat
     8521 cttcttgcgg ggttaactga atcacctgac gcttcatccc tttttcagct tgtggataag
     8581 gcgcgatttt ttccagtggc tggacgcttt ctgccgccca ggcggaagtg gtggcgaaag
     8641 cggcaaacaa tactgcaggt agaatggtct tcattttttt gctttccttc ttcatttata
     8701 ggttttcacc gatgatgtcg ctgaaggtta acatattcat cagatttatt aatttgcttt
     8761 atgaataact taaagaacat tcagacaatt ttacctgctc ttttagtgtt aaattctgat
     8821 gagggagcaa accagcagaa atatctctgg tgaattaatc gctatataaa tatgtttata
     8881 accatttgaa atgtgagcaa aagcccgttt ttgccacgct tcccgccact cttttgatcc
     8941 tgctacaggt tttaccccga tcggggtatg catctttgac acatccttta atatcttagc
     9001 ggctataaaa atggcttatt aattatgcgg tttatttggt cgctctcaat tttcagagcg
     9061 cgttaatgat ggaaggtcaa tgtgaagatt gatgcatccc gtcggggcat actcactggt
     9121 cgctggcgca aagccagtaa cggtatccgt ccgccctggt ccggtgatga atctcatttt
     9181 ctgacccatt gcacccgttg cgacgcctgt attaatgctt gtgaaaacaa cattctgcaa
     9241 cgcggcgcgg gcggctatcc gagcgttaat ttcaaaaata acgagtgcag cttctgttac
     9301 gcctgcgcgc aggcttgccc cgaatcgcta ttttctccgc gccacaccag ggcctgggat
     9361 ttgcagttca ccattgggga cgcgtgcctg gcgtatcagt cagttgaatg ccgccgctgt
     9421 caggatagct gtgagccaat ggcaattatc tttcgcccga cgctctccgg gatctaccag
     9481 ccgcaactta atagccaact ttgcaacgga tgtggagcat gtgcagccag ctgtccggta
     9541 tcagccataa ctgcggagta tctccatgca cactaactgg caagtttgca gcctggtcgt
     9601 gcaggccaaa agcgaacgaa tttcagacat cagcacccaa ctgaacgcct ttcccggctg
     9661 tgaagttgct gtcagcgacg cgccgagcgg tcagttgatt gtggtggtgg aagcagaaga
     9721 cagcgaaacg ctgatccaaa ccattgagtc agtacgcaac gtagagggcg tgctggcggt
     9781 gtcgctggtt tatcaccagc aggaagagca aggtgaggaa acaccatgaa actcagtcgt
     9841 cgtagcttta tgaaagctaa cgccgttgcg gccgctgcgg cggctgccgg tctcagcgtg
     9901 ccgggcgttg cccgcgccgt tgttggtcag caggaagcca ttaaatggga taaagcgccg
     9961 tgccgtttct gcggtactgg ttgcggcgtt ctggtcggaa cgcagcaggg gcgtgtggtg
    10021 gcctgtcagg gcgacccgga cgcaccggtt aaccgtggcc tgaactgcat taagggctat
    10081 ttcctgccca agatcatgta cggtaaagac cgtttgacgc agccgctgct gcgtatgaaa
    10141 aacggtaaat atgacaaaga aggcgaattt accccaatca cctgggatca ggccttcgat
    10201 gtgatggaag agaaattcaa aaccgccctg aaagaaaaag ggccggaatc gatcggtatg
    10261 ttcggttctg gtcagtggac tatctgggaa ggttatgccg cgtccaagct gtttaaagcg
    10321 ggcttccgtt cgaacaacat cgacccgaac gcgcgtcact gtatggcgtc ggcagtagtt
    10381 ggctttatgc gtacctttgg tatggatgag ccgatgggct gctatgacga catcgagcag
    10441 gctgacgcgt ttgtgctgtg gggcgcaaac atggcggaga tgcacccgat cctctggtcg
    10501 cgcatcacta accgtcgtct ctctaatcag aacgtcaccg tggcggtgct ttctacctac
    10561 cagcatcgta gcttcgagct ggcggataac ggcatcatct ttacgccaca gtctgacctg
    10621 gtgatcctga actacatcgc caactatatc attcaaaaca atgcgataaa tcaggacttc
    10681 ttcagcaaac acgttaacct gcgcaaaggg gcgacggaca tcggctacgg tttacgtccg
    10741 acccatccgc tggaaaaagc agcgaagaat ccaggttctg acgcctccga accgatgagc
    10801 tttgaagatt acaaagcctt cgttgccgag tatacgctgg aaaaaaccgc tgaaatgacc
    10861 ggcgtgccga aagaccagtt agaacaactg gcgcagctgt atgccgatcc gaacaagaaa
    10921 gtcatctcct actggacgat gggcttcaac cagcatactc gtggcgtgtg ggctaacaac
    10981 ctggtctaca acctgcacct gctgaccggc aaaatttccc agccgggttg cggtccgttc
    11041 tccctgaccg ggcaaccttc tgcgtgtggt actgcgcgtg aagtgggcac ctttgctcac
    11101 cgtctgcctg cggacatggt ggtgaccaac gagaaacacc gtgatatctg cgagaagaag
    11161 tggaatatcc cgagcggcac cattccggcg aaaatcggtc tgcacgcggt ggcacaagac
    11221 cgtgcgctga aagacggcaa actgaatgtt tactggacca tgtgtaccaa caacatgcag
    11281 gccgggccga acatcaatga agagcgtatg ccgggctggc gcgatccgcg caacttcatc
    11341 atcgtctccg atccgtatcc gacagtcagt gcgctggccg ccgacttgat cctgccgacc
    11401 gcaatgtggg tagagaaaga gggtgcttac ggtaacgccg aacgccgtac tcagttctgg
    11461 cgtcagcagg tacaggcacc gggcgaagcg aaatcggatc tctggcagtt agtccagttc
    11521 tcccgccgct tcaaaactga agaagtgtgg ccggaagagc tgctagcgaa gaaaccggaa
    11581 ctgcgtggca aaacgctgta cgaagtgctt tatgccacac cggaagtgag caaattcccg
    11641 gtatccgaac tggcggaaga tcagctgaac gatgaatccc gcgagctggg cttctatctg
    11701 caaaaagggc tgttcgaaga gtacgcatgg tttggtcgcg gtcacggtca cgatctcgcg
    11761 ccgttcgatg actaccacaa agcgcgcggt ctgcgctggc cggtggtgaa cggtaaagaa
    11821 acgcagtggc gttacagcga aggtaacgac ccgtacgtga aagcgggcga aggctataag
    11881 ttctacggta aaccggatgg taaagcggtg atcttcgcgc tgccgttcga accggcggcg
    11941 gaagcaccgg atgaagagta cgacctgtgg ctctctaccg gacgcgttct ggagcactgg
    12001 cacactggca gtatgactcg ccgtgtgccg gaactgcacc gcgccttccc ggaagcggtc
    12061 ttgtttattc acccgctgga tgcgaaagcg cgcgatctgc gccgtggcga caaagtgaaa
    12121 gtggtttctc gccgtggcga agtgatctcg attgttgaaa cgcgcggtcg taaccgtccg
    12181 ccacagggcc tggtgtacat gccgttcttc gacgccgcac agctggttaa caaactaacg
    12241 ctggacgcga ccgatccgct ctcgaaagag acggacttca agaagtgcgc agtcaaactg
    12301 gagaaggtgt aagccattat gtcccggtca gcgaaacctc aaaatggtcg ccgccgcttt
    12361 ctgcgcgatg ttgttcgcac agcaggcggg ctggctgccg tgggtgtggc gctggggtta
    12421 caacagcaaa ccgcacgcgc atctggcgtg cggttgcgcc cgcccggagc cataaacgag
    12481 aacgcctttg ccagtgcctg tgtgcgttgc ggtcagtgtg ttcaggcttg cccttacgac
    12541 accttaaaac tggcgacgct ggcttctggc ctgtcggcgg gcacgccata ttttgtcgca
    12601 cgggatattc cttgcgaaat gtgtgaggac attccgtgcg ccaaagtgtg cccgagtggt
    12661 gcgctggatc gtgagatcga atcgatcgac gacgcgcgga tggggctggc ggtgctggtg
    12721 gaccaggaaa actgtctcaa ctttcagggg ctgcgctgcg atgtttgtta tcgcgaatgc
    12781 ccgaaaattg atgaggccat caccctggag ctggagcgca acacgcgtac cggtaagcac
    12841 gcccgctttc tgccgacggt tcacagcgac gcctgtaccg gctgcggtaa gtgcgaaaaa
    12901 gtgtgcgtgc tggaacaacc ggcaatcaag gtgttaccgc tgtcactggc gaaaggggag
    12961 ttaggtcacc attaccgctt cggctggctg gaggggaaca atggcaaatc gtaaacgtga
    13021 cgccgggcgc gaggcgctgg tgaaaaaagg ctggtggcgc agtcaccgtt ggctggtgtt
    13081 acgtcgcctt tgtcagttct tcgtgctggg gatgtttttg agcggtccgt ggtttggtgt
    13141 gtggatctta cacggcaact acagctgtag cctgttattc gacaccgttc cgctgaccga
    13201 tccgctgatg acgctgcaaa gtcttgccag tggtcatctg cctgccacgg tggcgttgac
    13261 cggtgcggtg ataatcactg tgctctacgc cctggcagga aagcgattat tttgcagctg
    13321 ggtctgcccg ctgaacccga ttaccgactt agcgaactgg ttacgcaggc gctttgacct
    13381 caatcagtct gcgacgatcc ctcgccacat tcgctacgtg ctgttggtgg tcattctgct
    13441 gggatcagcc ttaaccggca cgctcatctg ggaatggatc aaccccgttt ccctgatggg
    13501 gcgtagcctg gtaatgggct tcggcagcgg cgcgctgctt attctcgcgc tgtttttatt
    13561 tgatctactg gtcgttgaac acggctggtg cgggcacatt tgtccggttg gcgcgctgta
    13621 tggcgtgctg ggtagcaaag gtgtgattac cgttgcggcc agcgatcggc agaaatgtaa
    13681 ccgctgtatg gattgttttc atgtttgccc ggaaccgcat gtgctacgtg ccccggtgct
    13741 ggatgaacaa agcccggtgc aggtcaccag ccgcgattgc atgacgtgcg gtcgctgcgt
    13801 ggatgtctgt tctgaggatg tatttacaat aactacacga tggagttcgg gagcgaaatc
    13861 atgaaaagcc atgacctgaa gaaagcgctg tgtcaatgga cggcgatgct ggccctggtg
    13921 gtaagcggcg cggtttgggc ggctaatggc gtcgatttta gccagtcgcc ggaagtatcc
    13981 gggacgcagg aaggggccat tcggatgcca aaagagcagg atcggatgcc gctgaactat
    14041 gtgaatcagc cgccaatgat cccgcatagc gttgaaggtt atcaggtaac gaccaatacc
    14101 aaccgctgct tgcagtgcca cggtgtcgaa agctatcgca ccactggcgc gccgcgtatc
    14161 agtcctactc actttatgga cagcgacggc aaagtgggcg cggaagtggc tccgcgtcgt
    14221 tatttctgtc tgcaatgtca cgtaccgcag gccgataccg cgccaatcgt ggggaatacc
    14281 tttaccccat caaaaggtta cgggaaataa gaggttatta tgggaaattc tgaccgtaag
    14341 cctggtctga ttaagcgcct gtggaaatgg tggcgtaccc ccagccgtct ggcgctggga
    14401 acgctgctgt tgatcggttt tgtcggcggc atcgtcttct ggggcggctt taacaccgga
    14461 atggaaaaag ccaataccga agagttctgc attagctgcc acgaaatgcg taacacggtg
    14521 tatcaggaat acatggattc cgtgcactac aacaaccgta gcggcgtccg tgcgacctgt
    14581 ccggattgtc acgttccgca cgagtttgtg ccgaagatga tacgcaagct caaagcaagt
    14641 aaagagctgt atggtaaaat ttttggcgtt attgacacgc cgcagaaatt tgaagctcat
    14701 cgtctgacaa tggcacagaa tgagtggcgg cgcatgaagg acaataactc gcaggagtgc
    14761 cgtaactgtc acaacttcga gtatatggat acaaccgccc agaaatcggt tgccgcgaag
    14821 atgcatgacc aggcggtgaa agatgggcaa acctgtattg attgccataa agggatagcg
    14881 cacaagctgc ccgatatgcg tgaagtcgag ccaggttttt aacagggtta ttgcgtgggt
    14941 atgcttgaag ccagagagtt actttgtgag cgggatgaac gaaccttatt tagtggcttg
    15001 tcatttacgc tgaacgcagg agagtgggta caaatcaccg gtagcaacgg cgcggggaag
    15061 acaacgcttc tccgtttgct gacggggttg tctcgccctg acgcaggcga ggttctctgg
    15121 caagggcagc ccttgcatca ggtacgcgac agctaccatc aaaacctgtt atggataggc
    15181 catcagccgg ggatcaaaac ccggctgacg gcgttagaaa atctgcactt ttatcatcgc
    15241 gatggcgata ccgcacaatg tctggaagcc ctggcgcagg ccgggcttgc cggattcgac
    15301 gatattccgg taaatcaact ttctgccggg caacaacgcc gcgtcgcttt agcgcgtctg
    15361 tggctgaccc gtgccacgtt atggatcctc gacgagcctt ttaccgcgat tgacgttaac
    15421 ggcgtcgatc gtctgaccca gcgtatggcg cagcatacgg agcagggggg gattgtgatt
    15481 ctgactaccc accagccgct caacgttgct gaaagtaaaa ttcgccgcat ttcactgacg
    15541 caaacgaggg ccgcatgatg ttctggcgca ttttccgtct tgagctgcgt gtagcgtttc
    15601 gccatagcgc cgaaatcgcc aacccgctgt ggttcttcct gattgtaatt accctttttc
    15661 cgctcagtat cggtccggag ccgcaactgc tggcgcgtat tgcaccgggc attatctggg
    15721 ttgctgcgct gctttcatcc ttgctggcgc tggaacgact gttccgtgac gatttgcagg
    15781 acggcagtct tgaacaattg atgttgttgc cgttaccctt gcccgccgtt gtgctggcga
    15841 aggtgatggc gcactggatg gtaaccggtc tgccgttact catcctttcg ccactggtag
    15901 caatgctact gggaatggat gtttatggct ggcaagtgat ggcgctgacg ctgctgctgg
    15961 gaacgcctac gcttggcttt ctcggtgcac cgggtgtggc gttgacagtg ggacttaagc
    16021 gcggtggtgt gctgctcagc atactggtgt taccgctgac tatcccatta ctcatctttg
    16081 ccaccgccgc gatggacgcg gcttccatgc atttgcccgt tgacgggtat ctggcaattt
    16141 taggcgcgtt gctggcaggc accgcgacat taagtccttt tgcgacggcg gcagcgttac
    16201 gaatcagcat tcaataacgc tggattactg atttttgttc gagtctggta tcgaaactat
    16261 gtggaaaaca ctgcatcaac tggcgatccc accacggctg tatcaaatct gtggctggtt
    16321 tataccgtgg ctggcaattg ccagtgtggt cgtgcttacc gtcggctgga tctggggatt
    16381 cggctttgct ccggctgatt atcagcaggg aaatagctac cgcattatct acctgcatgt
    16441 gcctgcggcg atctggtcga tgggcattta tgcatcaatg gcagtggcag cgtttattgg
    16501 ccttgtctgg cagatgaaaa tggccaacct ggcggtggcg gcgatggccc ccattggtgc
    16561 cgtgtttacc tttattgccc tggttaccgg ctctgcatgg ggaaaaccga tgtggggcac
    16621 ctggtgggta tgggatgcac gtctgacttc tgaactggtg ctgctgtttt tgtatgtggg
    16681 tgtgattgcc ctgtggcacg ccttcgacga ccgccgtctg gcgggccgtg cggcaggtat
    16741 cctggtgctg attggcgtgg tgaatctgcc gattattcat tactccgtgg agtggtggaa
    16801 caccctgcat cagggatcaa cgcggatgca gcaaagtatc gatccggcga tgcgttcgcc
    16861 gctgcgctgg tcgatttttg gcttcctgct cctgtctgcc acgctgacgc tgatgcggat
    16921 gcgtaatttg attttgctga tggaaaaacg ccgtccgtgg gtgagtgaac tgatactgaa
    16981 aagaggccgt aaatgacccc tgcatttgct tcctggaatg aatttttcgc aatgggcggt
    17041 tacgcctttt ttgtctggct ggcggtggtg atgaccgtta ttccgctggt ggttttggtc
    17101 gtgcactcgg tgatgcaaca tcgcgcaatt ctgcgtggtg tggcgcaaca gcgggcgcgt
    17161 gaggcgcgtt tacgtgctgc gcaacagcag gaggctgcat gaatattcgc cgtaaaaacc
    17221 gcttgtggat tgcctgtgcc gtgttggcag ggctggcgct gactatcggt ctggtgctat
    17281 atgcgctgcg ctcgaatatc gatctctttt atacgccggg ggaaattctc tacggcaagc
    17341 gtgaaactca gcaaatgccg gaagtcggtc agcgtttgcg cgttggcggg atggtgatgc
    17401 tgggtagtgt gcagcgcgat cccaattcgc tgaaagtgac cttcactatt tacgatgctg
    17461 aaggctcagt ggatgtctct tacgaaggca ttttgccgga tctgttccgt gaagggcagg
    17521 gcgttgtggt gcagggcgag ctggaaaaag gcaatcatat cctcgcgaaa gaagtgctgg
    17581 cgaaacacga cgaaaactac acgccgccag aagttgagaa agcgatggaa gctaaccacc
    17641 gtcgcccggc gagtgtttat aaggacccag catcatgatg ccggaaattg gaaacgggct
    17701 gctgtgtctg gcgttaggga ttgcgctgct gctgtccgtg tatccgctat ggggcgtggc
    17761 gcgcggagat gcgcgcatga tcgcgtcttc ccgcttgttt gcctggctgc tgtttatgtc
    17821 tgtggctggc gcatttctgg tgctggtcaa tgctttcgtg gtcaacgact ttaccgtcac
    17881 ctatgttgcc agctactcca atacccagct tccggtgtgg tatcgcgtgg cggctacctg
    17941 gggcgcgcat gaaggctcgc tactgctgtg ggtgctgctg atgagcggct ggacctttgc
    18001 ggtggcgatt tttagtcagc gtattccgct ggatattgtg gcccgcgtac tggcgataat
    18061 ggggatggtc agcgtcggct ttttgctgtt cattctcttt acctctaacc cattctcacg
    18121 cacgctgccg aacttcccga ttgaagggcg cgatcttaac ccgctgttac aggacccggg
    18181 gctgatcttc catccgcctc tgctttatat ggggtacgtg ggtttctcgg tggcgtttgc
    18241 ttttgccatt gcttctttgc tgagcgggcg tctggacagc acttatgcgc gttttactcg
    18301 cccgtggacg ctggcggcgt ggattttcct gacgctcggc attgtgctcg gttccgcatg
    18361 ggcctattac gaactcggct gggggggctg gtggttctgg gatccggtag aaaacgcctc
    18421 gtttatgccg tggctggtgg ggactgcgct gatgcactca ctggcggtca ctgaacaacg
    18481 cgccagcttc aaagcgtgga cattactgct ggcaatcagt gccttctcgt tgtgtctgtt
    18541 ggggactttc ctcgtgcgtt ccggcgtgct ggtatcggta cacgcgtttg cgtctgatcc
    18601 ggcgcgcggt atgtttatcc tcgcctttat ggtgctggtg attggaggtt cgctgctgct
    18661 gtttgccgcg cgtggacaca aagttcgctc acgcgtaaac aatgcgctgt ggtcgcggga
    18721 atctctgctg ttagcgaaca acgttttgct ggtcgccgcg atgctggtgg tgttgctggg
    18781 gacgctgctg ccgctggtgc acaagcaact gggactgggc agtatttcga ttggcgaacc
    18841 gttcttcaac accatgttta cctggctgat ggtgccgttt gcgctactgc tgggtgtcgg
    18901 tcctctggtg cgctgggggc gcgatcgccc acgtaaaatc cgcaatttat tgattatcgc
    18961 cttcatctca acgctggtgc tgtctctgct gttgccgtgg ctgtttgaga gcaaagttgt
    19021 ggcgatgacg gtgctcggcc tggcaatggc ctgctggatt gcggtgctgg caattgcgga
    19081 agctgcgctg cgtatttcac gcggcacgaa aaccaccttc agttattggg ggatggtggc
    19141 tgctcacctg gggctggcag tgacaattgt tggcattgct tttagccaga actatagcgt
    19201 tgagcgtgat gtgcgcatga agtccggcga tagcatcgat attcatgaat atcgcttcac
    19261 cttccgtgat gtcaaagagg tgactggccc gaactggcgt ggcggtgtgg cgactatcgg
    19321 cgtaacgcga gagggtaaac cggaaacagt gctgtatgcg gaaaaacgtt attacaacac
    19381 tgccgggtcg atgatgaccg aagcggcgat tgacggtggc atcacgcgtg acctgtacgc
    19441 cgcgctcggt gaagagctgg aaaacggcgc gtgggctgtg cgtctttact acaaaccatt
    19501 tgttcgctgg atttgggcgg gcgggctgat gatggcgttg ggcggactgc tgtgtctgtt
    19561 tgatcctcgc tatcgtaagc gcgtgagtcc gcaaaaaact gcgccggagg ctgtatgaag
    19621 cgcaaagtat tgttaattcc gttgattatc ttcctggcga ttgccgcggc gctgctgtgg
    19681 cagctggcgc gtaatgccga aggggatgat ccgaccaatc tggaatcggc gctcattggc
    19741 aagccagtgc cgaagtttcg cctcgaatca ctggacaatc cggggcagtt ttatcaggcg
    19801 gatgtgctga ctcagggcaa accagtgctg cttaacgtct gggcgacctg gtgtccgacc
    19861 tgccgtgcgg aacatcaata tctgaatcag ctttctgcgc agggcatccg cgtagtcggc
    19921 atgaactata aagacgatcg ccagaaggca atcagctggc tgaaagagct gggcaatcct
    19981 tacgcgctaa gcctgtttga tggcgatggc atgttagggc tggatctcgg tgtctatggc
    20041 gcgccagaaa cgttccttat tgacggcaac ggcatcattc gctatcgcca tgcgggcgat
    20101 ctcaatcctc gcgtctggga agaagagatc aagccgctgt gggagaaata cagtaaggag
    20161 gccgcacaat gaggttttta ttgggcatgc tgatgctgat gatctccggc tcagcgctgg
    20221 cgaccataga cgtgttgcag tttaaagatg aagcacagga acaacagttc cgtcagctca
    20281 ctgaagaact gcgctgcccg aaatgccaga acaacagcat tgccgattcc aactcgatga
    20341 ttgccaccga cctgcgccag aaagtgtatg aactgatgca ggaaggtaaa agtaaaaaag
    20401 agattgtcga ttatatggtg gcgcgttacg gcaacttcgt cacttacgat ccgccgttaa
    20461 cgccgctgac cgtgctgctg tgggtgctgc cagtagtagc tattggcatt ggcggttggg
    20521 tcatatacgc ccgctcgcgg cgtcgggtac gcgtggtgcc ggaagcgttt cctgaacaaa
    20581 gcgtgccgga aggtaagcgt gccggatatg tcgcttattt gccgggcatt gtggtggcgt
    20641 taattgtggc tggcgtcagc tactaccaga ccggaaatta tcaacaggtg aaaatctggc
    20701 agcaggccac ggcacaggct ccggcgttgc tggacagggc gctggatccg aaagctgatc
    20761 cgctcaacga agaagagatg tcgcgccttg cgctggggat gcgtactcaa ctgcaaaaaa
    20821 atccgggaga tatagacggc tggattatgt tgggccgcgt tggcatggcg ctgggtaacg
    20881 ccagtatcgc caccgatgca tacgctactg cgtatcgcct cgatccgaaa aacagtgatg
    20941 ccgcactggg ttatgctgaa gcgttgacac gttcatctga tcccaacgac aaccgcctcg
    21001 gcggcgaact gctacgtcag ctggtgagaa cggaccatag caatatccgt gtgctaagca
    21061 tgtatgcgtt taatgccttt gagcagcagc gatttggcga agccgttgcc gcgtgggaga
    21121 tgatgttgaa actcttacct gccaacgata ctcgccgtgc ggtgattgaa cgtagtatcg
    21181 cgcaggcgat gcaacatttg tcgccgcagg agagtaaata aaaagaggcc cgatggtgat
    21241 gccatcgggc tatttttatt gtgccccgcg ttgttgcagg aacagaatgg tggccgccac
    21301 gcgtgagcgg acattgagtt tacgcagcag atttcgaata tgaactttta ctgtctgctc
    21361 ggaaatattc aacacagagg caatctgttt atttgacagc ccctgtgcca gctcgtgcag
    21421 aacatccagc tcgcgctccg tcagcacgct gaagggatct tcttccgcgc caaacatttc
    21481 acgttcacgt aagtactgat tgacgcgttc gctaaagact ttgctgcctt tcgctccggc
    21541 acgaatcgct tccagcaaca cttccgggtc gctgtctttc agcagataac cgtctgcgcc
    21601 tgcgtctatc agcgcaaaga catcgctgga ggcatcggat acggtcagga taataatttg
    21661 cgcggtaacg ccatccctgc gcaaggcatt gagggtatcc aggccactca tacctttcat
    21721 attgagatcc agcaagatca cgtcgatatc cagtctattc gccagatcga tcgcgctcgc
    21781 gccgtcgccc gcttcggcga ccacttcaaa gccaggatca agctccagta actgacgaac
    21841 accgcgtcgc ataagtggat gatcatccac aatcatcacc tgaaaaggcg ttgcttcagg
    21901 catagtagtc tcctgaggtt ttattagaat cattatgttt gagcgtagcg ttggtaacgc
    21961 taccaggcgc gtgaatgtta ccccatttta tgtatggaca tgtagtgaaa actacaacct
    22021 tgccgttcgc tttggcatca ttttcattat aaagacggaa ttttgcatca gcttctcatc
    22081 aagaaaaaca actaaaaaat caatcacaat acttgtgcat ttctttaaac agtttttaaa
    22141 aactgactcc gggtatggag ctatgggtat tttctgtacc caatgctttt aacagcaatt
    22201 aatttcatag gatgaaagct caatgcatca atctggttct gtttctcttt gtcgttccgc
    22261 aatatctgtt ctggtggcta cagcgttata ttcacccata gcattggcat caacagttga
    22321 gtatggtgag acagttgatg gtgttggcct ggaaaaagat atccagctgg tttatgggac
    22381 cgccaataat acgaaaatca atcctggcgg agaacagcat ataaaagaat ttggtgtaag
    22441 tagtaatact gaaattaacg gcgggtatca gtacattgaa atgaatggca ctgcagaata
    22501 ctcagtatta aatgatggtt atcaaattgt tcaaatgggt ggcgcggtaa accagactac
    22561 gctcaataat ggagtgctac
//
LOCUS       DAJVQG010000061        22236 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJVQG010000061 DAJVQG010000000
VERSION     DAJVQG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22236
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.061"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            75..1463
                     /locus_tag="OQM83_002069"
     CDS             75..1463
                     /locus_tag="OQM83_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCS1520335.1"
                     /translation="MSAYLRYIVQKADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPE
                     QRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEG
                     NRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRK
                     DIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTH
                     DRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFIT
                     SSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPELEQRLQK
                     LVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDE
                     FCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIGFSSGIYAMKENDTLHDAYKASDER
                     LYVNKQNKNSRS"
     gene            1506..2009
                     /gene="yeaK"
                     /locus_tag="OQM83_002070"
     CDS             1506..2009
                     /gene="yeaK"
                     /locus_tag="OQM83_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCS1520336.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(2010..2114)
                     /locus_tag="OQM83_002071"
     CDS             complement(2010..2114)
                     /locus_tag="OQM83_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1520337.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            2284..2730
                     /locus_tag="OQM83_002072"
     CDS             2284..2730
                     /locus_tag="OQM83_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCS1520338.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="OQM83_002073"
     CDS             complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="OQM83_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCS1520339.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3605..4786
                     /gene="nimT"
                     /locus_tag="OQM83_002074"
     CDS             3605..4786
                     /gene="nimT"
                     /locus_tag="OQM83_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HCS1520340.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            4841..5188
                     /locus_tag="OQM83_002075"
     CDS             4841..5188
                     /locus_tag="OQM83_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCS1520341.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="OQM83_002076"
     CDS             complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="OQM83_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCS1520342.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5647..6672
                     /gene="dgcP"
                     /locus_tag="OQM83_002077"
     CDS             5647..6672
                     /gene="dgcP"
                     /locus_tag="OQM83_002077"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCS1520343.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            6705..6803
                     /locus_tag="OQM83_002078"
     CDS             6705..6803
                     /locus_tag="OQM83_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCS1520344.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6806..6880
                     /gene="yoaJ"
                     /locus_tag="OQM83_002079"
     CDS             6806..6880
                     /gene="yoaJ"
                     /locus_tag="OQM83_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCS1520345.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6939..7187)
                     /locus_tag="OQM83_002080"
     CDS             complement(6939..7187)
                     /locus_tag="OQM83_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCS1520346.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(7335..7517)
                     /locus_tag="OQM83_002081"
     CDS             complement(7335..7517)
                     /locus_tag="OQM83_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HCS1520347.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(7521..7880)
                     /gene="yeaR"
                     /locus_tag="OQM83_002082"
     CDS             complement(7521..7880)
                     /gene="yeaR"
                     /locus_tag="OQM83_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HCS1520348.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(8053..8691)
                     /gene="leuE"
                     /locus_tag="OQM83_002083"
     CDS             complement(8053..8691)
                     /gene="leuE"
                     /locus_tag="OQM83_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HCS1520349.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(8818..9741)
                     /gene="dmlR"
                     /locus_tag="OQM83_002084"
     CDS             complement(8818..9741)
                     /gene="dmlR"
                     /locus_tag="OQM83_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HCS1520350.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            9844..10929
                     /gene="dmlA"
                     /locus_tag="OQM83_002085"
     CDS             9844..10929
                     /gene="dmlA"
                     /locus_tag="OQM83_002085"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HCS1520351.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVAIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            11180..12790
                     /gene="yeaV"
                     /locus_tag="OQM83_002086"
     CDS             11180..12790
                     /gene="yeaV"
                     /locus_tag="OQM83_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HCS1520352.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYPPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            12822..13946
                     /gene="yeaW"
                     /locus_tag="OQM83_002087"
     CDS             12822..13946
                     /gene="yeaW"
                     /locus_tag="OQM83_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HCS1520353.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            14002..14967
                     /gene="yeaX"
                     /locus_tag="OQM83_002088"
     CDS             14002..14967
                     /gene="yeaX"
                     /locus_tag="OQM83_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCS1520354.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(15021..16136)
                     /gene="rnd"
                     /locus_tag="OQM83_002089"
     CDS             complement(15021..16136)
                     /gene="rnd"
                     /locus_tag="OQM83_002089"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCS1520355.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(16218..17903)
                     /gene="fadD"
                     /locus_tag="OQM83_002090"
     CDS             complement(16218..17903)
                     /gene="fadD"
                     /locus_tag="OQM83_002090"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCS1520356.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(18108..18689)
                     /gene="yeaY"
                     /locus_tag="OQM83_002091"
     CDS             complement(18108..18689)
                     /gene="yeaY"
                     /locus_tag="OQM83_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCS1520357.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(18729..19424)
                     /gene="tsaB"
                     /locus_tag="OQM83_002092"
     CDS             complement(18729..19424)
                     /gene="tsaB"
                     /locus_tag="OQM83_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCS1520358.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(19482..21392)
                     /locus_tag="OQM83_002093"
     CDS             complement(19482..21392)
                     /locus_tag="OQM83_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCS1520359.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            21524..21868
                     /locus_tag="OQM83_002094"
     CDS             21524..21868
                     /locus_tag="OQM83_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCS1520360.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5444 a         5699 c         5404 g         5689 t
ORIGIN      
        1 attttatcgc cgcaagtgtc attgtgctga catcttcctt ccttattttt gaacttgtcg
       61 ccagcgacag agcaatgagt gcctatctgc gctatatcgt gcagaaagca gactcctcct
      121 ttctttatga taagtatcag aatcagagta ttgccgcgca tgtgatgcgc gctctcgctg
      181 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
      241 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      301 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      361 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatgg
      421 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      481 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      541 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      601 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      661 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      721 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      781 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      841 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      901 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      961 tccgaatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
     1021 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
     1081 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagcaaataa
     1141 atgacaccct cggtcatcag gagggggatt tagcgattac gttattagct caggcgatta
     1201 aacaatcgat tcgtaaaagt gattatgcca tccgactcgg tggcgatgaa ttctgcatca
     1261 ttcttgtcga ttcgacgccg caaattgcag cacaactgcc tgaacgtatc gaaaaacgtc
     1321 tgcaacatat cgcgccgcag aaagaaatcg gcttctcttc cggtatttac gcgatgaaag
     1381 aaaacgatac gttacatgat gcgtataaag cttccgatga gcgtttatat gtcaataagc
     1441 agaacaaaaa cagccgttca tgataacctt ctgtggttgt ttgcttgtaa tctcaggagc
     1501 gtgaaatgac tgaaatggct aaaggaagcg tgacgcatca gcggttaatc gctttattat
     1561 cacaagaagg tgctaacttc cgcgtcgtta cccatgaagc ggtaggtaaa tgtgaagcgg
     1621 tgtcagaaat tcgtggcacc gctttaggcc agggagctaa ggcactggta tgtaaagtca
     1681 aaggcaatgg cgtaaatcaa catgttctgg cgatcctcgc tgccgatcaa caggccgatc
     1741 tgagccaact tgccagccat ataggcggat tacgcgcttc gttagccagc cctgctgaag
     1801 tcgatgaact cacaggctgt gtcttcggcg cgatcccccc tttcagcttc catccaaaac
     1861 tcaaactggt tgccgaccct ctactcttcg aacgatttga tgaaatcgcc ttcaatgcag
     1921 ggatgctgga taaatccgtt attttgaaaa ccacggatta tttgcgcatt gcgcaaccag
     1981 aactgctcaa tttccgccgc actgcgtaac tagcctgtcc gttcaataag cagaacggac
     2041 aaaaccagca caacagcaat ggcaaaaaac gatgacgtga taatcagtgt ctcgacgaac
     2101 atttgatcgt tcatagcatg cccccgggag atagctgttt tcacgttatt gttagcgtgc
     2161 acaaatggca gtttgatgac aattcgcctg attttttatg caaaaaacgt aaagattttc
     2221 tacttccttc ctgcagcaaa cgtaaagtaa gcaggcttat tattttttgg caaggaaacc
     2281 acgatgtttg atgtcacttt gctgatcctg ctcgggttag ctgcgctggg ctttatcagt
     2341 cataacacca ctgtcgccgt ttcaattctg gtgttgatca ttgtccgcgt cacaccgtta
     2401 agcacctttt tcccgtggat tgaaaaacag gggctaagta tcgggataat cattctgacg
     2461 attggcgtta tggcccccat cgccagcggg acgctaccgc cctcaacctt gattcactcg
     2521 tttctgaact ggaaatcgct ggtggcaatt gcagtaggcg tgattgtctc ctggctgggt
     2581 gggcgcggcg tgaccttaat gggcagccag ccgcaactgg tcgccgggct gctggtaggc
     2641 actgttttag gcgtggcgct gtttcgcggc gtaccggtcg gcccgcttat tgccgcgggt
     2701 ctggtttcgc tgattgtggg gaaacagtag ttaatcccgc aatatatcgg cctggcgttt
     2761 gccccaggcc tttcttaaac atagtaataa aggccgttgt tgaatcatac cccagcgtat
     2821 gagcaacttt ctgtacagta tcgcctttca ctaatccctg aagtgccata atcagctgta
     2881 actgctgacg ccactggcga aaactcaacc ccgtctcttt tactatcagt cgcgcaagat
     2941 tgcgttcact catcgcaaaa aagccagccc attgccctaa tgacccccac tcgacgggcc
     3001 ctttcgccat catctccacc atcgtgcgga ttttaggatg agaagaaacg ggcaggtgca
     3061 gctgttgttg tggctgctga gggagttcat caaacaggac ctgaattaat ctgcgggtca
     3121 tcggctcagc acgctgagtc gtcgtcctgt ttgccagggt taaaatcaat tcccggcata
     3181 agggcgatat tttcaatgtg caacatgttg tcggcattgt cactgcgctg ggttcgataa
     3241 acagaaaaca gagttcagcg ttagctgtca cttgattgct atgttccacc ccgcccggta
     3301 tccaaaccgc atattgcggc ggcaccatcc ataaagcatt ttccaccgta caggtaattg
     3361 caccatgtaa tgctagtatc agctgtcctt tgcggtgctg atgtacagga ctggttaact
     3421 catccgttcc ggcgtgaata caaaatgcga ccgcggcttc atggtgtcgg tcgggttcat
     3481 agccattgag attcaacctg tgcatcattt tgtccgaatt tagcgataat ttgtcatttt
     3541 agcttgattc aacataacaa taaaaacggt aaggtacagc ctcgtttgta acaatgagaa
     3601 gcatatgacc tgttcaactt cattaagcgg caaaaacagg attgtcctta tcgctggcat
     3661 tctgatgatt gccacaacat tacgcgtcac ctttatcggc gcagcaccgt tactggatac
     3721 gattcgttcc gcttactcgc tgacgacagc gcaaaccggc ttattgacca ccctgccatt
     3781 attggccttt gcgctaatct cacctttggc caccccggta gcgcgacgtt ttggtatgga
     3841 acgtagcctg tttgccgcgt tacttttgat ctgtgctggt atcgcaattc gctctctccc
     3901 ttcgccttac ttattatttg gcggtacagc ggtcattggc ggtgggattg cattaggcaa
     3961 tgtcttactg ccaggattaa ttaaacgcga tttccctcat tccgtcgcca gacttaccgg
     4021 cgcatattcc ctgacaatgg gagctgcagc ggcactggga tcggctatga tagtgccgct
     4081 ggctttgaac ggttttggct ggcaaggcgc gttgctcatg ctgatgtgtt ttcctctgct
     4141 ggctcttttt ttatggctgc cacagtggcg aagccaacaa catgcaaatt tgagtacctc
     4201 gcgcgcctta catactcggg gtatctggcg ttcgccgctt gcctggcagg taacattgtt
     4261 tcttgggatc aactcactga tctattacgt gattattggc tggcttccgg cgatcctcat
     4321 cagtcacggc tatagcgaag cacaggcggg ttcactgcat ggtttgctgc aactagccac
     4381 agcagctccc ggtttgctga tccctctttt cttacatcat gtgaaagatc agcgtggtat
     4441 tgcagcgttc gttgccttga tgtgcgcagt gggcgctgtt gggctctgct ttatgccagc
     4501 gcacgcgatc acctggactc tgcttttcgg ttttggttcc ggcgcaacaa tgatactggg
     4561 gttgacgttc attggtcttc gggcaagttc tgcgcatcag gcggcggcac tctcggggat
     4621 ggcacaatcc gtcgggtatt tgctggcagc ctgtgggccg ccgctaatgg gtaaaataca
     4681 cgatgctaac ggtgactggt ccgtaccact gatgggtgtt gcaatacttt cactactgat
     4741 ggcgattttc ggactttgcg ccgggagaga caaagaaatt cgctaatatc cggtgctata
     4801 gtgacgtgac aaatcatgcg tgaaagggag aacaaacacg atgaatattc agtgcaaacg
     4861 cgtttatgat ccggctgaac agagcgatgg ttatcgcgta ctggtcgacc gcctctggcc
     4921 gcgcggtatc aaaaaaaccg atttagccct tgatgagtgg gataaagaaa tcacgccgtc
     4981 aacggaactg cgcaaagcct ttcacggcga agtcgtcgat tttgcaacct ttcgcgagca
     5041 atatcttgca gaactggcgc aacacgagca agaaggaaag cggctggcgg acatcgccaa
     5101 aaaacagccg ctgaccctgc tctacgcagc aaaaaacacc acgcagaacc atgcgctggt
     5161 gctggccgac tggctacgta gcttgtgatt ttagtacagc atccggcggt tatttttcac
     5221 cagccggatg gtcccgtcgc cacaatgccc attcatcaat cgtttcaccg cccggtaatt
     5281 tgcaattgtt gctgacccct tgcgctgtcg acactggaat gagcgtcccg cccttctgct
     5341 ggcaatagac cgacgccgga tttgccatac caatctgcgg cggtttaggt acttctggct
     5401 gagaagaggt tgaacaacca gccaggacca gcaagcaagg caaagcaaaa ctgataattt
     5461 tcatttattg atctcactca tttatctacg cttagagtat cgcggtatcg ttttgttttg
     5521 cagcactatt tttattacat tcactcaaaa catattacgt cttgtttcat ctttgttggt
     5581 gatgttttat catgcctgca aagattaaat aatcagcatt tacccgccgt atcctggagt
     5641 tgtttcgtgt cagatcagat tatcgcccgc gtctcgcaat cccttgccaa agaacagtca
     5701 ctggaaagtc tggtccgaca gcttctggag atgctggaaa tggtcactga tatggaatca
     5761 acctacctga ccaaagtgga tgtcgaagcg cgcctgcagc atattatgtt tgcccgtaac
     5821 agccagaaaa tgcacatccc ggagaatttt accgtctcgt gggattactc gttatgcaaa
     5881 cgcgccattg atgaaaactg ctttttcagc gatgaagtcc ctgaccgttg gggcgactgt
     5941 attgcggcac gcaatcttgg catctccaca tttctgagca caccaattca cttaccggat
     6001 ggatcattct atggcacgct ttgcgccgcc agcagtgaga agcgccagtg gagtgaacgc
     6061 gcggaacagg ttttacagtt attcgccgga ctgattgcac aatatattca aaaagaggca
     6121 ctggttgaac agttgcgcga agccaatgct gcgctgattg cgcaatcgta taccgactcg
     6181 ttaaccgggt tgccgaatcc gcgggcgatt tttgaaaatc tgacgacgct gttttctctc
     6241 gcccggcatc ttaaccataa gataatgatc gcgtttatcg atctggataa cttcaaatta
     6301 atcaatgatc gttttggtca taatagtggc gatctgtttc tcattcaggt tggcgagcgc
     6361 cttaatacgc tccagcaaaa tggcgaagtt attggtcgtc tcggcggtga tgagtttttg
     6421 gttgtttcac taaacaacga gaatgcggat atttcgtcgc tgcgagaacg cattcagcag
     6481 caaatacgtg gagaatatca cttaggtgat gttgatttgt attatcccgg tgccagtctt
     6541 ggcatagtag aagtcgatcc tgaaacaacc gatgcagaca gtgccctgca tgctgccgat
     6601 attgcgatgt atcaggagaa aaaacacaaa cagaaaacac cttttgtcgc gcattcagcg
     6661 ccacattgct gaggcgtatt cacatccttt tgattggtga taacatgcga atcggtatta
     6721 tttttccggt tgtaatcttt attacagcgg tcgtattttt agcatggttt tttattggcg
     6781 gctatgctgc cccgggggca taaagatgaa aaaaacaacg attattatga tgggtgtggc
     6841 gattattgtc gtactcggca ctgagctggg atggtggtaa cgtcacctct aaaaaatagc
     6901 aaaggctgcc tgtgtgcagc ctttgtgcaa tttaagcgtt aacttttaat cttcctgtag
     6961 ataaatagca cgacaatcgc accaataacg gcaaccacga agctgccaaa attgaagcca
     7021 tcgactttac caaagccaaa cagcgtgctg atccatccgc cgactacggc accgactatc
     7081 cccagcagga tagtcataaa gaatccacca ccatctttac ctggcatgat ccacttcgcc
     7141 agaataccgg caataagccc aaaaataatc catgacagaa tgcccattgt ttcctcactt
     7201 atctgttttg cattagcggg ttagtcgctg ataaaaagca tagcacaaca tcgggagggc
     7261 aagatttgtg acgagcatca cggaggattt ttttgcgatg gcgcagaaat tgcgccatca
     7321 acgatcagtg ataattacca accacaaaca tcatgttcgt tttccgtgtc ataagaacgc
     7381 acggtattca ccagatcttt tatcacttca gccgccactt ctggcaccag caaagtcatc
     7441 ggcgtctctg tttcataatc gacagaaacg ccattactgt tattggtgac ggtcacggta
     7501 tacgttgctt tgcccatgat tcatttcccg ttatgaatga ctttccgttg ttgcgcacct
     7561 tccatcagga cttcaggagc cacgaagaag tcaatgttga aataagtatc gtcagtcatg
     7621 gcttcaatgt tgtgccactt ttctggaggg aacacctcaa actgccccgc ttcgataagg
     7681 atcacctgat caggctctgc actgtgttca tcagcgtagc cgagatattt gaccgcccca
     7741 tgcataacgg aaaggcgtgg gtaaaccccc gggcgcgttc ctttatcaag atgacgttcg
     7801 aatattccgg caggcgcagt ttgtttattc cagaaaggcg ttgagcgcgt atgaatataa
     7861 ttctgtggga tttgcagcat ccttttccct ccttcggtga atgcgctgaa aacggtttat
     7921 tccagccgtt tcagggtacg cctgataatt tgcattttaa ataccattta ttggttactt
     7981 tttagcacca tatcagagaa aaatcaggga ggattacaag tgggaagccc gcgacaacgc
     8041 gggctgaatg tatcaggatt gcagcgtcgc cagtcgggcg gcgaaaccca cgaacatcaa
     8101 accaatcagt gagttgccaa ctttagccag tttctttttg gtacgtatgt actgcgtgac
     8161 aaaagcacca gaaataatca ggaagctcaa atagcagaaa ctcaccagtt ccagcgtcgt
     8221 cgccagaata aagaatgaaa ttcccgtatg tggggcatta acatcgataa actgtacgaa
     8281 aaacgacaca tagaacaaaa tggctttcgg attagtcagg ctcaaaatta acgcgcgttt
     8341 aaaaatggcc ccgtattggg gctcatcgga tttggcctcg ttatttttac ccttcagggt
     8401 cgcgtagaga attttactcc ccagatagag caaataaaac gcaccaagat aacgtacaat
     8461 gttgaataat atcggggtgg tcttaattaa tgtcgccact ccagcccatg ccagaaacat
     8521 caatactgca tcgccaataa atacaccgca ggccgcaaga taaccgcctt tcataccgct
     8581 actgacgcta tttttgagta caaacagggt atttggccct ggcaccaaca caataaaaat
     8641 ggccccaacc agataggtcc agtaattcag aaccccgtat tcagcgaaca cattaacctc
     8701 tttaattatc tttcgatcat gcgcgattaa aggtgaatat gctaaccaat ctgtagcggc
     8761 ttagaaagga gaaaatcagg ttttaacccg atatcaaccc gataattgaa taattaatca
     8821 ggcatgctcc agtgaaaaat tcgggtagtg ctcggcaaaa tactggcgta aaaattctat
     8881 cgttatccgc actttcgctg acgtcgccag ccttgaaaca taaacggccc agacgttcgc
     8941 tggctggtaa tattccggta gcacttgcac taaatgacca ctggcaatgt tttcgctaac
     9001 atcccaccag gagcgcagag caatcccctg cccatcgaga caccactgat ggacaatttc
     9061 tccatgatta gacgacagtg ggccggtgac tttaatcgca tggtggcctt ctttattgcg
     9121 taattgccag acaccaaaag gatgatcgcg ctctttaatg accaggcacg gcaatgcgga
     9181 aagatcggtt aaatgtttcg gcgcgccatg ttgcgcaata aattccggcg acgcgcagag
     9241 aatgcggtaa ttagtcgcca gtttgcgggc gattaaatta ggggcgatgt catcgccaat
     9301 gcgaatatcg agatcaacac cttcattgac caaatcgacc aaccggtctt ccacatcaaa
     9361 acgtaattcc agttgcggat acgccttagc cagcgctgat aacgccggag ccaccacctg
     9421 tcgtccaaaa ccaaagctgc tgataatacg cagcatcccc tgcggcacct gacgcacgtc
     9481 agaaagttcg tccatcatct gaccgacatc ctgcaaaatc cgctgcgccc attcataaat
     9541 ccgctctcct tcttcggtaa tggtgacgcg gcgggtggtg cggtgtaaca acaccacgtt
     9601 tagcgtttgc tccagcaagg cgatgcgctt gctgacgaac gccggtgaaa cgcccagttc
     9661 ttccgccacg gcggcaaaac cagcccggcg agccaccagc ataaagacgc gcaaatcgtt
     9721 cagcagcggt aaattattca tgattcgtgt tttatgtttc accagttacg gggattaatt
     9781 ccttttcagt caattatagg atggtgatgt tgtcaatttt gatggtcagg aagtgagaac
     9841 ccaatgatga aaacgatgcg tattgctgcg atcccgggag atgggattgg caaagaagtc
     9901 cttcctgaag ggattcgcgt attacaggct gccgctgagc gctggggctt cgacttgagt
     9961 tttgagcaaa tggagtgggc gagctgcgag tattacagcc atcacggtaa aatgatgccg
    10021 gacgactggc atgagcaact tagccgtttc gacgccatct attttggtgc cgtcggctgg
    10081 ccggataccg ttccggacca tatttcgttg tggggttcgc tgctgaaatt tcgtcgtgaa
    10141 ttcgaccagt acgtcaacct gcgcccggtt cgtctctttc ctggcgttcc ctgcccgctg
    10201 gcgggaaaac agcctggcga catcgatttt tacgtggtca gggaaaacac cgaaggcgaa
    10261 tattcctcgc tcggcggtag agtgaatgaa ggtacagagc atgaagtcgc cattcaggaa
    10321 tcggtattta cgcgtcgtgg tgtcgatcgc attttgcgtt atgccttcga acttgcgcaa
    10381 agccgcccac gtaagacgct aacttctgcc actaaatcaa acggtttagc catcagcatg
    10441 ccgtactggg atgagcgagt ggaagcaatg gccgagaatt acccggagat ccgctgggac
    10501 aagcagcata ttgatattct ctgcgcgcgt tttgtgatgc agccggaacg cttcgatgtg
    10561 gtggtggcgt ccaatttgtt tggcgatatc ctttccgatc ttggcccggc ctgcaccggc
    10621 accattggca ttgccccatc cgccaacctg aatccggaac gcactttccc gtcgctcttc
    10681 gagcctgtcc acggttccgc gccggatatc tacgggaaaa atattgctaa ccctatcgcc
    10741 acaatttggg ccggtgcgat gatgctcgat tttctcggca atggcgatga gcgtttccag
    10801 caagcgcata acggtattct ggcagcgatt gaagaagtga ttgctcacgg gccgaaaaca
    10861 ccggatatga aaggcagtgc caccacgcca caggttgccg acgcgatttg caaaattatt
    10921 ttgcgttaag gtcaaaccag tttatttgaa ccgcgtcact gacgcggttt ttttattcgt
    10981 tctttgcagt taataacctg cgtcatttca ccttttattg tttccgtttc gtgttttatg
    11041 gctttccgta ttcttaattg tttaatttat gtaacatgca aataatcaat ttgttaaaca
    11101 caacatacaa ctcattaccc tgcaaataat tacaactggc aaaagccaga gacgggcttt
    11161 tatgcccagg agatgactca tgatgagcaa tgttaagaaa aaagatgtgc cgctgataag
    11221 catcagcctg gtggccattc ttttcatcgc agctgcatta agccttttcc cacaacaatc
    11281 ggccgacgcg gccaacacaa tatacacttt tgttacgcgt acgttaggtt ccgccgtaca
    11341 ggtattggtt ttgctggcaa tgggactggt gatttattta gccaccagta aatacggcaa
    11401 tattcgtctt ggcgaaggaa aaccggaata cagcacgctc tcctggctgt ttatgtttat
    11461 ttgtgccggt ttaggttctt ctacgcttta ttggggggtt gctgaatggg cctattatta
    11521 tcaaacgcct ggattaaata tcgcaccacg ttcacaacag gcactcgaat ttagcgtccc
    11581 ttactccttc ttccactggg gcatcagcac ctgggcaact tatacgctgg cctcattaat
    11641 catggcttat cactttcatg tgcgtaaaaa caaaggtctg agtctttccg gcattattgc
    11701 cgcgattacc ggcgttcgcc cgcaaggccc atggggaaaa ctggttgatt tgatgtttct
    11761 gatcgccact gtcggcgcac tgactatttc ccttgttgtc accgcagcaa cctttactcg
    11821 tggactttcc gcgctgaccg gtttacccga taacttcacc gtgcaggcat ttgtgatcct
    11881 gctttccggc ggcatttttt gcctaagctc gtggattggt atcaacaacg gtttgcaacg
    11941 tctgagcaaa atggttggct ggggcgcgtt cctgctgcca ttactggtgc tgattgtcgg
    12001 tccaaccgaa tttattacca acagcatcat caatgccatc ggcctgacca cgcaaaactt
    12061 cctgcaaatg agcttattca ccgatccgct tggcgatggt tcatttaccc gcaactggac
    12121 cgttttctac tggctgtggt ggatctcata cccccctggc gtagcaatgt ttgtcacccg
    12181 cgtttcccgc ggtcgtaaga ttaaagaagt tatctgggga ctgatcctcg gcagcaccgt
    12241 cggttgctgg ttcttcttcg gcgtaatgga aagctatgcc attcatcagt ttatcaatgg
    12301 cgtaatcaac gtcccacagg tgctggaaac actgggcggc gaaacagctg tacagcaagt
    12361 tctgatgtcg ttgccagccg gtaaattgtt cctcgccgca tacctgggcg tgatgattat
    12421 tttccttgcc tcgcatatgg atgcagtggc ctacaccatg gcggcgacca gtacgcgtaa
    12481 tctccaggaa ggtgacgatc ctgaccgtgg gctgcgtctt ttctggtgcg tggtgatcac
    12541 tctgatcccg ctttccatct tgtttaccgg tgcttcgctg gaaacgatga aaaccaccgt
    12601 cgtgctcaca gcccttccct tcctcgtcat tttactggtg aaagtcggcg gatttattcg
    12661 ctggctgaaa caggattatg ccgacattcc ggctcatcaa gttgaacatt atctcccgca
    12721 gacaccggtt gaagccctgg aaaaaacacc agtgctccct gcgggaaccg tattcaaagg
    12781 cgacaactga gcgccatcat cctaacgata aaggtatccc tatgagcaat ctgagccctg
    12841 actttgtact acccgaaaat ttttgcgcta acccgcaaga ggcgtggacc attcctgccc
    12901 gtttttatac cgatcagaac gcgtttgaac acgaaaaaga gaacgtcttc gccaaaagct
    12961 ggatttgcgt cgctcacagc agcgaactgg cgaatgccaa tgattatgtg acgcgtgaga
    13021 tcattggcga aagcatcgtg ctggtacgcg gtcgtgataa ggttttgcgc gcgttctata
    13081 acgtgtgtcc gcaccgtggt catcagttgt tgagcggtga aggaaaagca aaaaatgtga
    13141 ttacctgccc atatcacgca tgggcattca aactcgatgg caatctggcc catgcacgta
    13201 actgcgaaaa cgtcgccaat ttcgatagcg acaaagcgca actggttccg gtgcgtctgg
    13261 aggaatatgc cggattcgtc ttcatcaaca tggaccccaa cgccaccagc gtagaagatc
    13321 agttacccgg tctgggcgcg aaagtgctgg aagcctgccc ggaagtccac gatctgaaac
    13381 tggcggcccg ctttaccacc cgcacgcctg ccaactggaa gaacattgtc gataactatc
    13441 tcgagtgcta tcactgtggt ccggcgcatc caggtttctc cgactccgta caggttgatc
    13501 gctactggca caccatgcac ggtaactgga cgctgcaata cggtttcgcc aaaccgtccg
    13561 aacagtcgtt taaatttgaa gagggtacgg atgcggcatt ccacggtttc tggctgtggc
    13621 cgtgcacgat gctgaacgtc accccgatca aagggatgat gacggtcatt tatgaattcc
    13681 cggtggattc tgaaactacc ctgcaaaact acgatattta cttcaccaat gaagagttaa
    13741 ccgacgagca aaaatcgctg attgagtggt atcgcgatgt gttccgtccg gaagatttac
    13801 gtctggttga aagcgtacag aaagggctga aatcgcgtgg ctatcgtggt caggggcgca
    13861 tcatggccga cagtagcggt agtggcattt ccgaacatgg tatcgcccat ttccataatc
    13921 tgctggcgca ggtgtttaag gactaatgac atcggcggcg gtattttccg ccgctgggct
    13981 gatttttgat ggagtacagc aatgtcagac tatcaaatgt ttgaagtaca ggtgagtcag
    14041 gtggaaccgc ttaccgaaca ggtgaaacgc ttcacgctgg tggcaaccga tggcaaaccg
    14101 ctgcctgcgt tcaccggagg aagtcacatc attgtgcaga tgagcgatgg tgataaccag
    14161 tacagcaatg cgtattcact actgagttcg ccgcatgaca cctcttgtta tcagattgcc
    14221 gttcggctgg aggaaaactc gcgcggcggt tcccgctttt tgcatcagca ggtaaaagtg
    14281 ggcgatcggt taacgatttc aacgcctaat aacctgtttg cgctaattcc ctcagccaga
    14341 aagcatctgt ttatcgcggg cggtattggt atcacccctt tcctgtcgca catggcagag
    14401 ttgcaacaca gcgacgtcga ctggcagcta cattactgct cgcgaaatcc agaaagttgc
    14461 gcatttcgtg atgagctagt ccagcatccg caggctgaga aagtccattt gcatcattcg
    14521 tcaaccggaa cacgactgga attagcgcga ttattggcgg atatcgaacc tggcacacac
    14581 gtttatacct gtggccccga ggcgctaaat gaagcggtaa gaagtgaagc tgcgcgtctg
    14641 gacatcgccg ccgatacgct gcactttgag caatttgcta tcgaagacaa aaccggcgat
    14701 gcctttaccc tggtgcttgc ccgttccgga aaagagtttg tggtgccgga agagatgact
    14761 attttgcagg ttattgaaaa taataaagcc gcgaaagtgg aatgtttatg tcgtgaaggg
    14821 gtatgcggaa cctgcgaaac agcaatactg gaaggtgaag ctgaccatcg ggatcagtat
    14881 tttagcgatg aagagcgtgc cagccagcaa agtatgttga tctgttgttc gcgtgcgaag
    14941 ggtaaacgcc tggtgttgga tttgtagttt gccaaagccg gatgtggcgc tgagcgcgcc
    15001 cagtccggct tcggaagatt ttactgcgga tattcctgca ataaattgtg taatgcttcc
    15061 gccatcagct caccacgcca gccggaaatc agctccggca aattgttctg cggtttcagt
    15121 ttccagtgcc agttcagcag ttggttgatt tgccgacgcg atgccagcaa ttcggcgctg
    15181 atcttatgcg tttcgctcac gtcagtaatc agcgacttaa tcgctttaaa cgctttacga
    15241 taacccggca tgtccatcag gttaagcatc ggctgcggta aggcctcttc cggcaatgtc
    15301 tgcgcttttt ccaccagcgc cagcagcgtt ttaccgtgaa agcggatttc gctaccggat
    15361 aaacccaggc tgtccagttc gcctaaactt cccggcatat aacgcgcgac cgaccacaaa
    15421 tgctcttcac gcacgacaaa gttcaccgcc agatcacgct cacgagcctt gcgcagtcgc
    15481 cagtcggcta acagttgcag acaggccagt tggcgtgtgc gtaattgcca ggcattggtg
    15541 atatcacgcc aggcatcttc cggcgcaacg acttcctgac gacgcatttg catcaggcgg
    15601 cattcatcca gcgccgcagg tagccagccg gaggcctccg tttctaccat aagcttggcg
    15661 gtgatcggta acagatacca gacatccgcc gctgcgtatt cacactgacg ttcggtcagc
    15721 ggtctggcca gccagtcggt gcgcgattca ctcttgtcca gcgtaacgcc ggaatactct
    15781 tccaccatgg aagcgaaacc ccatgacatc gggcgtccgc agaaggcagc aaggatttgc
    15841 gtgtcaatca ggggttgtgg taattcgcca aagacattga ggaacacttc cagatcttca
    15901 ctgcctgcat ggagaaattt tgtgatggac ggatcgcgca ggatcgcttt cagcggtgac
    15961 cagtcggtga tcccgagagg atcaattagc gccagatgct caccatcgaa aagttgaatc
    16021 aaccccagct gcgggtaata agtgcgcgta cgaacaaatt cagtatcctg ggctatcgcc
    16081 ggaaagacac ggacggcttc acacaaagaa gccagcgcat cgtccgtggt aatcatttgg
    16141 taattcaaat tgtattctct ttagtgggcg tcaaaaaaaa cgccggatta accggcgtct
    16201 gacgactgac ttaacgctca ggctttattg tccactttgc cgcgcgcttc gtcacgtaat
    16261 tctcgtcgca aaatttttcc gacgttagat ttcggtaact catcacgaaa ctccaccagc
    16321 ttcggtactt tgtagcccgt gagctgacgg cagcaaaagg tcaccagtga ctcttcggta
    16381 agcgatggat cttttttcac tacgaagatt ttcaccgctt caccactgga gccggaaggt
    16441 acgccaacag ccgcgacttc ctgtacgcca ggatgctgca tgacgacatc ttcaatctcg
    16501 ttgggataga cgttaaaacc ggaaaccaga atcatgtctt ttttacgatc gacaatgcgc
    16561 aggaaccctt cttcatccat caccgcgatg tcgccggtgt gtaaccagcc atttttgatg
    16621 atctcatcgg tagcatcggg acgctgccag taacccagca tcacctgcgg tcctttgaca
    16681 caaagctcac ccggttgacc cggtggtact tcattatcgt cgtcatccac cagtttggct
    16741 tccgtcgacg gcaccggcaa accgatgcta ccactatgat aatcaatatc atatgggtta
    16801 acgctgacca gcggcgcaca ctcggtaaga ccatagcctt ccagcagata ctgtcctgtc
    16861 agtttcaccc aacgctctgc caccacttgc tgcactggca tcccaccgcc tgcggaaaga
    16921 tgcagactgg agaaatccag ctgctggaac tctttattgt tcagcaacgc attgaacaag
    16981 gtgttaacgc cagtgatagc ggtaaacgga tatttcgcta actcttttac caaccctgga
    17041 atatcgcgcg ggttagtgat aagcaggttc tgcccaccca gttcgataaa cagcaggcag
    17101 ttaatggtca gggcaaaaat gtgatacagc ggcagtgccg ttactaccag ttctttacct
    17161 ggatgcagca acggcccata ggtggcgttg acctgttcca ggttcgccag catattgcgg
    17221 tgagtcagca tcgcgccttt cgccacacct gtggtgccgc cggtgtattg caggaaggct
    17281 aaatcttccg gcaccagttc gggtttgacg tactgcatcc ggtagccgtt atgcagtgcg
    17341 ctacgaaatg aaatggcatc tggcagatgg tatttcggca ccaaacgctt gatgtattta
    17401 acaacgaaat tgactaccgt gccttttgcc gtagatagct gatcgcccat acgggtcaga
    17461 attacgtgct gaacggcggt tttatcaacc actttttcca gtgtgtgagc aaagttagac
    17521 acgataacaa tcgccgatgc gccgctatcg ttaagctgat gctcaagctc acgcggggta
    17581 tacaacgggt taacgtttac gacgatcatc ccggcacgca aaatgccaaa cagcgccacc
    17641 ggatattgca ataaattagg catcatcaac gcaacgcgat cgcctttctt cagccccaac
    17701 ccttgttgca aataagcggc aaacgcgcga ctgcgttctt ccagcttgcg gaaggtcatt
    17761 acctccccca tattcacaaa cgcaggctga tcggcgtagc ggacgaccga ctgctcaaac
    17821 atatctatca gagattgata acggtcaggg ttgatctccg tcggaacatc cgcgggataa
    17881 cggttaagcc aaaccttctt caattcttca cctctaaaat gcgtgttcgt cgtcatcgca
    17941 accccaagtg ataaacatgt tgttaacata acattaactc atcttaccag cttgataatt
    18001 agacaacgaa aaggttgcga agcgcgtcac tatttatttt tatagttgcc gtatgaacgc
    18061 agaaacagcg gaccagccgc tgtttctttt tctttaaaaa caacttgtta ctcggttaca
    18121 attgtttcaa ctcttgcagg gccaggatta taccaacccc atccgccgta accatagggc
    18181 cagccgcgtc caccataaaa ccatggatca attggctgcg gcggcataat cacctgttga
    18241 gtgatatgcc agcgtttgta acctgttacc tgcataacca taaatttata tggtgcatta
    18301 tttactttgc catccactgt gccagtgatt ggcccgacaa ccgtcaccag ttgtccacga
    18361 aaatcgaccg gatcaagaaa accgttcaca tcggcataaa tgcgcccgcg tgaaggttct
    18421 cccaaggttg gtcttgctcc gctgtccagc ggtacggtag caatttccag gcgggttttc
    18481 ccttgctggt tttgtaccac aaccactttg ccgccaaagc gcgcctcctg accaacatat
    18541 aaatctggcg cattcatcac gcgtaccaga tcttgctgtg gtgttggact gctgccttta
    18601 atggcgtccg gcacagtgac acaaccgctc agcattagcg caaacgtacc tgccagtatg
    18661 cctttgataa cattcttttg aaccgccatg atgcgactcc tttttttctc agggcatact
    18721 cttaagattc attctttgcc cggaagtttc ttccatgcga cgttgttacg taaataaacc
    18781 ggttcggcat gttccaccgc caccgtttta ccctcggcaa acatctgaca cgcaatcggc
    18841 agcatatctt ccgccgctgg cagtaaaact tctccgtcgc gcaaaaccag cccgctctct
    18901 ttacggagat ccggccaggc ttgccagccc gtccctaccg ttacccattc gccggaaagc
    18961 tgttgcattc gttcatgaac gagttcgggt ttgagtacgg cttcggtttc ttcaccgcgc
    19021 cagataccgt tttcatcacg ctgatattcg gcccagtaaa cttcgcccat tcgcgcgtca
    19081 atggctgcca gcacgcgggt tgcgccgttt ttgcgccacg ccccctgcgc catcgtcatt
    19141 agcgtagaga caccaatcat cggtaactcc gcgccaagcg ccagcccttg cgcaatacca
    19201 ataccaatgc gcacgccagt aaagctaccg gggccgcgcc cgtaagccag agcgtttatg
    19261 tcagtcaggg aagttccgct ggtggtcagg atatcctgca ccatcggtaa gattcgttga
    19321 gtatgttcac gagggcaaag ctcaaaatga gcgttgacat taccgtcgtt ccacagagcg
    19381 acagagcaga cctctgtcgc ggtatcgata gccagaattc gcatgggtct tcgtgcttag
    19441 atcaataaaa aggcgcgcat cataccatac tccgtaacaa attacctgga ggatggtatc
    19501 gcaaggaaac gaaccgcacg ggcaatgtca cgggtgcgtg gcgcgggcgg cagactggcg
    19561 agaaacgtcg cgccgtaagg acgcatcacc agccgattgt cacaaattac cagcacgccg
    19621 cgatcgtcgg cgtcgcgaat cagtcgccct actccctgct tgagagtaat gaccgcatcc
    19681 ggcagctgca cttcatcgaa cgggtcgcca ccgcgcaaac gacaatcttc catgcgcgct
    19741 tttaacagta gatcatccgg cgaggtaaac ggcaatttgt cgataattac caatgacaat
    19801 gtatcgccac gcacgtccac cccttcccag aaactactgg ttgccacaag aagcgcgtta
    19861 ccggcgctga caaattgctg caacagttgc cctttgctgg tttccccctg caacaaaacg
    19921 ggaagcgtca tggtagcgcg gaactgctcg gccagatcgc gcatcatggc atgcgaggta
    19981 caaagcataa aacaacgacc gttgttagct tcgatgatcg gtcgcagcat tgccgccagt
    20041 tgccgtgcac aacccggttg attggtttgc ggcaggttgc gcggcacaca gagtaacgcc
    20101 tggcggctgt aatcaaacgg gcttggcaat agcaacgact ccgcctgttc tatcccaagc
    20161 cgcgaggtga aatgatgcag atcgtcgttc accgacagcg ttgctgaggt gaagatccag
    20221 ctaccgggct tttgcgccat taactctttg aatttatccg ccacgctgag cggcgtgaga
    20281 gccagagtaa aatggcgcga agtgcattcg taccagtagc tgtagcccgg ctgattgatc
    20341 tcttttagcc gcttcagccg tgtgcgatac aacgtggcgc gctcaaatgc cgcatccagc
    20401 aaggcggaac gccccagtga cagtttcgcc acgtcataac aaagttccag ggtgtcatcg
    20461 agcagtaaaa atgcccgctg aatttgcgga ttagctaaca gctcacgcag gttaccgcga
    20521 taacctggct caccgagttg cagacgaaaa tcctgtgcac tctgggcaag acgatcagcg
    20581 cacttttgta actgctgggt gtcttttaat tcggtgcggt aggcgatggt gatgtctttt
    20641 gccaggtcga gcagttgtcg actggagagt gactgaccaa aatactggct ggcaatgtcc
    20701 ggtagctggt gggcttcgtc gaagatcatg acgtccgctt cagggatcaa ctcgccaaat
    20761 ccactctctt taaccaccat atccgccaga aagagatgat ggtttaccac caccacatcg
    20821 gcgtccatcg cttttttacg tgctttgacc acaaagcaat ctttatacat cgggcagtcg
    20881 ctgccaagac agttgtcgtt ggtgctggtg accagcggcc acgcctgtga atcttccgcc
    20941 acgctgacgc aggtgctgat atcaccatcg actgtttgat tagaccagga gcgcagcagg
    21001 atcacatcgc ttaagatttg taccggcaga tcgccccccg ccagcgcctg ctgttcgaga
    21061 cgttcgaggc agaggtagtt tgagcgccct ttcagcagcg ccacgttgcc cgtatatttc
    21121 aatgcctttg agactgttgg caaatcgcgg ctgtagagct gatcctgcaa cgcttttgag
    21181 ccggtcgaga taatgacttt ctttttcgcc cgcagcgcag gagccaggta agcgtaggtt
    21241 ttgcccgtac cggttcctgc ttccaccacc agcggctggc ctttttctat cgcctgggtg
    21301 acggctaccg ccatctgtcg ctgtggttct cgcggcttaa agcctggtat cgctttcgcc
    21361 agctgaccgt ctggtgcaaa atcgtccgtc acactacccc ctgttgattt gaacagggat
    21421 tatgtcagga tgagggcgct ttcgccagtt gaagtggtga cggcaacctc acattgtggc
    21481 agtctttgca gcacgaaaat ggaagtataa tgaggacaaa atgatgacta tcgttcgtat
    21541 cgatgctgaa gcccgctggt ctgatgtagt aatccacaac aacacgctct actacactgg
    21601 tgtaccggaa aacctcgacg ccgatgcctt tgagcaaacc gccaacacgc tggcacagat
    21661 tgacgccgtg ctggaaaaac agggcagcaa taaatcgagc attctggatg ccaccatttt
    21721 cctggccgat aaaaacgact tcgcggcgat gaataaagcg tgggatgcct gggttgtcgc
    21781 gggtcatgcg ccagtgcgct gcacggtaca agcgggtttg atgaacccga agtataaagt
    21841 tgaaattaag attgtggctg cggtgtaagc tttatcgaag caaaataagt cagacgataa
    21901 tttatcgata atactggtcg gttttacata aatcgaccag agaataagat attactgttg
    21961 atgcttctgc taattcatta tttatattta taatttcaat tttatctata aaactaacta
    22021 tataaataaa cataaccatg ccgttgtgtt cgccctttga ttagcaactc tggttttctt
    22081 tcttagctga acaatctggt tatagcgtgc ggactgaccg ttaattttct ccttcttact
    22141 ggataacaca ccatgagctg ctacctttaa aacacctgaa tgtcaacagg ttaactcgtg
    22201 ccatatcgtt ataatccctt tgatattaga tgcaaa
//
LOCUS       DAJVQG010000062        22014 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJVQG010000062 DAJVQG010000000
VERSION     DAJVQG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22014
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.062"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..407)
                     /locus_tag="OQM83_002095"
     CDS             complement(<1..407)
                     /locus_tag="OQM83_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS1520361.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLS"
     gene            703..1479
                     /gene="exuR"
                     /locus_tag="OQM83_002096"
     CDS             703..1479
                     /gene="exuR"
                     /locus_tag="OQM83_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCS1520362.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1824..2486
                     /locus_tag="OQM83_002097"
     CDS             1824..2486
                     /locus_tag="OQM83_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1520363.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            2490..2873
                     /gene="mzrA"
                     /locus_tag="OQM83_002098"
     CDS             2490..2873
                     /gene="mzrA"
                     /locus_tag="OQM83_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCS1520364.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            3020..3388
                     /locus_tag="OQM83_002099"
     CDS             3020..3388
                     /locus_tag="OQM83_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HCS1520365.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            3426..3731
                     /locus_tag="OQM83_002100"
     CDS             3426..3731
                     /locus_tag="OQM83_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCS1520366.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3734..4138
                     /locus_tag="OQM83_002101"
     CDS             3734..4138
                     /locus_tag="OQM83_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCS1520367.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            4128..4427
                     /locus_tag="OQM83_002102"
     CDS             4128..4427
                     /locus_tag="OQM83_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCS1520368.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4613..5005
                     /locus_tag="OQM83_002103"
     CDS             4613..5005
                     /locus_tag="OQM83_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCS1520369.1"
                     /translation="MKKLEDVGVMVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            5075..6088
                     /gene="yqjG"
                     /locus_tag="OQM83_002104"
     CDS             5075..6088
                     /gene="yqjG"
                     /locus_tag="OQM83_002104"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCS1520370.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYISLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESTEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPQDEMFASVKKRHLT
                     KCL"
     gene            6353..6718
                     /locus_tag="OQM83_002105"
     CDS             6353..6718
                     /locus_tag="OQM83_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCS1520371.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            6960..7316
                     /locus_tag="OQM83_002106"
     CDS             6960..7316
                     /locus_tag="OQM83_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCS1520372.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(7367..8263)
                     /gene="yhaJ"
                     /locus_tag="OQM83_002107"
                     /pseudo
     CDS             complement(7367..8263)
                     /gene="yhaJ"
                     /locus_tag="OQM83_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            8368..9069
                     /locus_tag="OQM83_002108"
     CDS             8368..9069
                     /locus_tag="OQM83_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCS1520373.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            9092..9256
                     /locus_tag="OQM83_002109"
     CDS             9092..9256
                     /locus_tag="OQM83_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1520374.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(9390..10700)
                     /locus_tag="OQM83_002110"
     CDS             complement(9390..10700)
                     /locus_tag="OQM83_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCS1520375.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVRIETHNG
                     VVFTQQECVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(10728..12059)
                     /locus_tag="OQM83_002111"
     CDS             complement(10728..12059)
                     /locus_tag="OQM83_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCS1520376.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(12334..13698)
                     /gene="tdcG"
                     /locus_tag="OQM83_002112"
     CDS             complement(12334..13698)
                     /gene="tdcG"
                     /locus_tag="OQM83_002112"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCS1520377.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(13770..14159)
                     /locus_tag="OQM83_002113"
     CDS             complement(13770..14159)
                     /locus_tag="OQM83_002113"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCS1520378.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQACLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(14173..16467)
                     /gene="tdcE"
                     /locus_tag="OQM83_002114"
     CDS             complement(14173..16467)
                     /gene="tdcE"
                     /locus_tag="OQM83_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCS1520379.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(16501..17709)
                     /gene="tdcD"
                     /locus_tag="OQM83_002115"
     CDS             complement(16501..17709)
                     /gene="tdcD"
                     /locus_tag="OQM83_002115"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCS1520380.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(17735..19066)
                     /gene="tdcC"
                     /locus_tag="OQM83_002116"
     CDS             complement(17735..19066)
                     /gene="tdcC"
                     /locus_tag="OQM83_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCS1520381.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(19088..20077)
                     /gene="tdcB"
                     /locus_tag="OQM83_002117"
     CDS             complement(19088..20077)
                     /gene="tdcB"
                     /locus_tag="OQM83_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCS1520382.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(20176..21114)
                     /gene="tdcA"
                     /locus_tag="OQM83_002118"
     CDS             complement(20176..21114)
                     /gene="tdcA"
                     /locus_tag="OQM83_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCS1520383.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            21303..21647
                     /gene="tdcR"
                     /locus_tag="OQM83_002119"
     CDS             21303..21647
                     /gene="tdcR"
                     /locus_tag="OQM83_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCS1520384.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
BASE COUNT         5766 a         5623 c         5148 g         5477 t
ORIGIN      
        1 gagagatctt ttttatctgt ggcaacgtca tcaatactgg gaaccgcttc aaatttaacg
       61 cgataatttt tttctttttg ctctggttcc attgcaacaa agcgcacagt tttgctacta
      121 ccggctggta aagcgaattt aggtggcata acaaccagat gatttgcatc accggattta
      181 atctcaactt cattttcctg aggcgtcgat ggattatcga tacggaatac tgtcgccttg
      241 atgtactgaa cttcattact tttagatatt acgcgaacgt tgccttcacc ctgactatta
      301 atggaaacag ccataggata aacagtcata tttgactgtg ccaaaaaagg agcgaacacg
      361 aataaaagcg gcgtaagccg tgataagaga ttaattttcg ccaccatata aatttgatct
      421 tacaatattg tttccaaaaa gttggtgtat tagagatgta gatttattgt aagtcaatat
      481 aaaaaagcat ttttagttaa tattaaattt aaaggctggg aattaactta ctgaaattta
      541 gtagctttca caatgacgat acttattttt taggaattta taaatattca caatattaat
      601 tcaatacatt aattcaatca ccaaatttat atatgaccaa tggcataccc atgaataaag
      661 tggtatgata acttcgtccg cattccactc cgggagacgc atatggaaat cactgaacca
      721 cgccgtttgt atcaacaact tgccgctgac ctgaaagagc gcattgaaca gggcgtctat
      781 ctggtgggtg ataaactgcc tgccgaacgc tttattgccg atgaaaagaa cgtcagccga
      841 acggtagttc gtgaagccat catcatgctg gaagttgaag gctatgtgga ggtgcgtaaa
      901 ggttcaggta ttcatgtggt ttccaaccag ccgcgccatc agcaggcggc tgacaataat
      961 atggaattcg ccaattacgg tccgtttgag ttgcttcagg ctcgccagct catcgaaagt
     1021 aatattgccg agttcgcggc aactcaggta acgaaacaag acatcatgaa actgatggcc
     1081 attcaggaac aggcgcgcgg cgaacaatgc ttccgtgatt ccgagtggga tttacagttc
     1141 cacattcagg tagccctggc gacgcagaac tccgccctgg cggccatcgt tgaaaaaatg
     1201 tggacccagc gtagccataa cccgtactgg aaaaaactac acgaacacat tgattcccgt
     1261 accgttgata actggtgtga tgaccacgat caaattctca aggcgttaat tcgcaaagat
     1321 ccccatgctg ctaagctggc aatgtggcag catctggaaa acaccaaaat catgttattt
     1381 aacgaaacca gcgacgattt cgagttcaat gccgaccgct atctattcgc cgaaaacccg
     1441 gtagtgcatc tcgatacggc caccagtggc agtaaatgaa actcctgtcc gacaggcgct
     1501 gcgacgcgcc tgttcgcttc gtcggtaagc gaaaagtata aagtgtcagc ctgtgtaaat
     1561 cctctcgcca ccctcccctg cattcagcaa aatcagactc cacggacatg taattttgat
     1621 aacgaacaac gttgaccttt gttacaatta gattcaattt gaatttatgt ttttgaatgc
     1681 tttcttatct cacgatttaa cagggaatag ttcaggctgt gttgatgtat caaacccgca
     1741 gaacatacca aaacagcaat aacattgcgg tagtgcatct tttaaaacca gcgtggcgtt
     1801 aaccgattca ccaggaataa tgaatggaac ttttgaccca attgctgcaa gccctgtggg
     1861 cgcaggattt tgaaaccctg gccaatccat cgatgattgg catgttgtat tttgtcttgt
     1921 ttgtaatttt gttccttgaa aacggcttgc ttccagcggc ctttttaccg ggcgacagtt
     1981 tactggtatt ggtcggcgtg ttgattgcga aaggcgcgat gggttacccg caaacgattc
     2041 tgctgctgac cgttgccgcc agcctcggct gctgggtcag ctatattcag gggcgatggc
     2101 tgggcaatac ccgcaccgta caaaactggc tatctcattt acccgcgcat tatcatcaac
     2161 gcgcacacca tctttttcat aaacacggtt tatcggcgct gttaattggt cgctttattg
     2221 cgtttgtcag aacactgctg ccgacgattg ccgggttatc agggctgaat aacgcgcgct
     2281 ttcagttttt caactggatg agcggtctgc tgtgggtatt gatcctgaca actctgggtt
     2341 acatgctcgg caaaacgccg atatttttaa agtacgagga ccagctgatg tcatgcctga
     2401 tgctgctccc ggtggtgctg ctggtgtttg gcctggcagg ttctctggtc gtgttatgga
     2461 aaaagaaata tggaaatcgg gggtaaggga tgcaaatacc tcgcatgtcg cttcgccagc
     2521 tagcctggtc cggcactgtt ttacttctgg tcggcacgct gttgctggcc tggtccgcgg
     2581 ttcgccagca agagtctacg ctggcgattc gtgccgttca tcaaggcaca acgatgccag
     2641 acggtttttc gatctggcat caccttgacg ctcatggcat tcctttcaaa agtatcaccc
     2701 ccaaaaacga cactctgtta attacatttg actccagcga ccagagcgcc gccgcaaaag
     2761 cggtcctcga cagaacattg ccccatggct acatcattgc gcagcaggac aataacagtc
     2821 aggctatgca gtggctgacc cggttacggg ataactctca tcgcttcgga taacttcctg
     2881 ttatccgaaa catatcactc actttggtga tttcaccgta actgtctatg attaatgagg
     2941 cggtggttaa cactaccgct tcgttgtctg gacaatcgtt cctttgtaat aggtccgaat
     3001 cacaatggaa ggttcaagaa tgaaataccg catcgcttta gctgtttctc tctttgctct
     3061 tagtgccggt agttatgcca ctaccctgtg tcaggaaaag gagcaaaata tccttaagga
     3121 gatcagctat gccgaaaaac accaaaacca gaatcgtatt gacggtctga ataaagccct
     3181 gagtgaagtc cgggccaact gttcagatag ccagctgcgt gccgatcatc acaagaaaat
     3241 cgcaaagcag aaagatgagg tggcggaacg ccagcaagat ttagccgagg cgaagcaaaa
     3301 aggcgatgcc gataagattg ccaaacgcga acggaaactg gcagaagcgc aggaagagct
     3361 gaaaaagctg gaagcgcgcg actactaact cacaatagtc actacttact cacctggaga
     3421 aaactatgtc gaaagaacac actacggaac atctgcgtgc tgagttgaaa tccctttccg
     3481 atacgctgga agaggtgctt agctcatctg gcgagaagtc gaaagaagag ttgagtaaga
     3541 ttcgtagcaa agcggagcag gcactgaaac agagccgtta tcgcctgggt gaaaccggtg
     3601 atgccatcgc caaacaaacc cgtgtcgcgg cggcgcgtgc tgatgagtat gtgcgtgaaa
     3661 atccgtggac cggcgtggga attggcgctg caatcggtgt agtgctcggc gttctgctgt
     3721 cgcgtcgtta attatggcgg acactcatca cgcgcaaggg cccggtaaaa gcgttctggg
     3781 catcgggcag cgaattgttt ccatcatggt tgaaatggtg gaaacacgtc tgcggctggc
     3841 ggtggtggag ctggaagagg aaaaagctaa tctctttcaa cttttactga tgctgggcct
     3901 gacgatgctt ttcgctgcat ttggtcttat gagcctgatg gtgctaatta tttgggcggt
     3961 tgatccgcaa tatcgcctga atgcgatgat tgccaccacc gtggtgttgc tgctactggc
     4021 actgattggc ggtatctgga cgttacgtaa atcgcgtaaa tccaccttgc tgcgccatac
     4081 acgccatgag ctggcaaacg atcggcaact gctcgaggag gagtcccgtg agcagtaaag
     4141 tcgaacgtga acgacgtaag gcgcaactgc ttagccagat ccagcaacaa cggctggatc
     4201 tttccgccag tcgtcgtgaa tggctggagg caacaggcgc ttacgatcgt cgctggaata
     4261 tgctgctaag tctgcgctcc tgggcgctgg ttggcagtag cgtgatggcg atctggacga
     4321 ttcgccatcc taatatgctg gtccgctggg ccagacgcgg ttttggcgta tggagcgcct
     4381 ggcgtctggt taaaacgacc ctcaagcagc aacagcttcg cggttaactt ccctctggcc
     4441 ggagcaattc cggccttatc cctcaaattt tttgaagatt tttgacagtt ttccttgcta
     4501 acaatcatca ttcaccacgt ttatgattct ctccatcgac agcaacgacg ctaataccgc
     4561 gcccttgcac aaaaaaacaa tcagcagcct gagtggcgca gtggagagta tgatgaaaaa
     4621 attagaagat gttggtgtaa tggtagcgcg cattttaatg ccgattctgt ttattaccgc
     4681 aggctgggga aaaatcaccg cttactctgg tacccaacaa tatatggaag caatgggtgt
     4741 cccgggtttt atgctgccgc tggtgattct gcttgagttc ggtggtggtc tggcaatcct
     4801 gttcggtttc ctgactcgca ccacagccct gtttactgcg ggctttacgc tgctgacggc
     4861 atttttattt cacagcaact ttgctgaagg cgtcaactcg ctgatgttca tgaaaaacct
     4921 gacaatttct ggcggattcc tgctgctggc aattaccggt ccgggcgcgt atagcatcga
     4981 ccgcctgctg aataaaaagt ggtaagcacg cgctatactt aacgataaaa aacgaggagg
     5041 aagctcctcg tttttgctat tggaggagag aaaaatgggt caactgattg acggcgtctg
     5101 gcatgacacc tggtacgata ccaaatctac cggcggtaaa tttcaacgtt cagcttccgc
     5161 atttcgtaac tggctcactg ccgatggcgc acctggcccc actggcacag gcggttttat
     5221 cgcagagaaa gatcgttatc atctctatat ttcactcgcc tgcccgtggg cgcaccgcac
     5281 gctgatcatg cgcaaactca aaggactgga accgtttatt tccgtttccg tagtgaaccc
     5341 gctgatgctg gaaaacggct ggacctttga tgacagtttt ccgggagcaa ccggagatac
     5401 gctctatcaa catgaatttc tgtatcagct ttatctccac gccgatccac actacagcgg
     5461 acgagttact gttcccgtac tgtgggacaa aaagaaccac accatcgtca gcaacgaatc
     5521 aacagaaatc atccgcatgt tcaataccgc gtttgatgcg ctgggcgcga aagcgggtga
     5581 ttactaccca ccagccctgc aaacgaaaat tgacgaactt aacggctgga tttatgacac
     5641 cgttaacaac ggcgtgtata aagctggttt tgccaccagc cagcaagcct acgacgaggc
     5701 ggtggcgaaa gtgtttgaat cgctggcgcg actggagcag attttaggcc agcatcgtta
     5761 cctgaccggc aaccagctaa ccgaagccga tattcgcctg tggactacgc tggtgcgttt
     5821 tgatccagtg tatgtgaccc acttcaagtg tgataagcac cgcatcagcg attacctgaa
     5881 tctgtatggc ttcctgcgcg atatctacca gatgccgggg atcgccgaaa ctgtcaattt
     5941 cgatcacatc cgtaatcatt acttccgcag ccataagacc atcaacccta cggggattat
     6001 ttcaattggt ccgtggcagg atctcgatga accgcaggac gagatgttcg cttcggttaa
     6061 aaaaaggcac cttacgaagt gcctctgata attagctaag tgcatgttgt tcatatcgga
     6121 tgcggcgtga acgccttatc aaacctacaa aacatttata ttcaatatgt tgcaatgacc
     6181 gcgcaggcca gataagcgta gcgcatctgg cgattttgcg cttgtcatca gtcttagcgc
     6241 gccttcacag cactttatta attccttgaa cgaatattta ctgccatttc attcacgtct
     6301 attcttaatt tgctgcttta aaaacaagtg attgagcaac attgaggcaa aaatggactg
     6361 gtatctgaaa gtaataaaaa attatgtcgg tttccggggt cgtgcgcggc gcaaagagta
     6421 ctggatgttt attctggtca acatcatctt tacgttcgtg ctggggctgc tggataaaat
     6481 gttaggctgg caacgcgctg gtggcgaagg catcctgacg acaatctacg gtattctggt
     6541 gtttttaccg tggtgggcag ttcagttccg ccgcctgcac gacaccgacc gctcggcgtg
     6601 gtgggcactg ctattcttaa tcccgtttat cggctggctt attatcatcg tctttaactg
     6661 ccaggcgggt acgccaggcg aaaaccgctt tggacctgat ccaaaactgg aaccttaaaa
     6721 caaatctgct cgccggatgt ctgactcatc cggcttttat catcatgttg atgaatgaat
     6781 aatttactaa tgtttattta aaatatttca atgtcgttat ataaattacc tataaaaaat
     6841 aaccatgtat ttatcttaaa tatcaaaacc tttataacat ccgaaaaaga aagatacgaa
     6901 tagcatttac acaatacccg gaatagactt tcgtcacttt taattaaagg gatgttttta
     6961 tgcagtggta tttatccgta ttaaaaaatt atgttggttt ctctggtcgc gcacgtcgta
     7021 aagagtactg gatgtttact ctgattaacg ccatcgtcgg cgctattatc aatgtcattc
     7081 aattgatttt aggtctggag cttccatatc tgtctatgct ttatttgctg gcaaccttcc
     7141 ttcccgtcct tgcgcttgct atccgtcgct tacacgatac cgaccgttca ggtgcctggg
     7201 ccttgctctt ttttgttccg tttattggct ggctcgtgct tctggttttt ttctgcacag
     7261 aaggtacttc tggcagcaat cgttacggaa acgatccgaa gtttggttca aattaatctt
     7321 agaattgggg cgatatttcg ccccttttta ttaacaataa taaatattat tttccgctaa
     7381 aaagtttggg aatttcccgc agacaccagg atttcgcttc ccccatactg tcacgacgcc
     7441 aggccataat aatatcgatc tcactggtcg attccgggct gacgacacgc aaccgccctt
     7501 ccgcaatatc tttttcgacc atcggatacg gcatcgtcgc cacgccaagc cccgccagta
     7561 atgcctgacg tttatcttca atcgtgctca ccgttaagcg cggctgtttg tccagcagtt
     7621 gtacggtcaa caccgggcgc tcacgagcgg tatccgctac cgcaattcca cgatatttca
     7681 cgcgcgtcac ttcagataac ggttccggct cctgatgaat cgggtgatca ggcgcggcaa
     7741 cgtagacgtt cattaacgta tagagcttgc gcgagttgat ctccgacgag gaacgaaaat
     7801 gcatatccgg cgcgataaca atatccgccc gcccctgctc cagtcgttcc tacgcccccg
     7861 ccagcacttc ggtgatgatt gccagttggg tattggcttt tgccgccagt ttgtcgatta
     7921 acgggaaaaa ggcaggtgtc ggtaccagcg cttcggtcac aatggtgaga tgcgtttccc
     7981 aaccgcgcgc gagggcttcc gcatcggtag tcagtttatc tgcggcttcc agcaaaacgc
     8041 gccctcgctc cagcaacatc ctcccgacat tggtgaattt ggtgcgatgg cccgagcggt
     8101 caaacagcac cacatccagc tcttcttcca gtttttgcat ggtgtagcta agtgcggaag
     8161 gcacgcgtcc cagctcatcc gccgccgccg caaaactgcc ccggcgatcg atcgcatcca
     8221 taacccgtag tgcttccagc gttaatgccc tttctttggc catttcgttc tcattcagga
     8281 aatttgaaca taccgggcag aatatctggc taacaatgta gcgtccagcc ccttaccata
     8341 aaaggaagta aagagaggtc aagaattatg attactaccc gaactgccag gcagtgtgga
     8401 caagcagact acggatggtt gcaggcccgg tatacttttt cctttggaca ctacttcgac
     8461 ccgaaattgt taggctatgc ctccctgcgt gtgcttaacc aggaagtgct ggccccaggt
     8521 gccgcctttc agccgcgaac ctatcccaaa gtcgatattt taaatgtgat tctggatgga
     8581 gaagcagagt atcgcgacag cgaaggcaat catgttcagg ccagcgccgg tgaggcgttg
     8641 ctgctctcta cccagccggg tgtcagctac agcgaacaca atctcagcaa agacaaaccg
     8701 ttaacacgaa tgcagctttg gctggacgcc tgcccgcagc gagagaatcc gctgattcaa
     8761 aaactggcgc ttaatatggg caagcagcaa ttaatcgcct cgccagaggg tacgatggga
     8821 agcctgcaat tacgccagca agtgtggctg caccatatcg tgctcgacaa aggcgaaagt
     8881 gcgaatttcc agttgcatgg gccacgcgcg tatttgcaat cgattcacgg gaaatttcat
     8941 gcgcttacgc atcatgaaga gaaagcggcg ctgacctgcg gtgatggggc gtttattcgt
     9001 gacgaggcta acattacgct ggttgccgat tccccactgc gcgctttgct gatagatttg
     9061 cctgtctagt tgtttttaca gggagatgat gatgagtaaa aaatcggcca aaaagcgcca
     9121 gccagtgaag cccgtggtgg cgaaagaacc tgctcgcacc gccaaaaatt ttggctatga
     9181 agagatgttg agcgagctgg aagccatcgt cgcggatgct gaaacgcgtt tagccgagga
     9241 tgaagctacc gcgtaaagac aggttgccgg atgcggcgaa aacgccttat ccggcctaca
     9301 aaatccaaca acattaacga attaaacaac ccgtaggccg gataaggtgc gccagcatcg
     9361 catccggcac aatactcaaa acctggcgtt tatctggcct tgctcgccat aatctcgata
     9421 atctgccgat ccgtttgctg catcgaatgg cttgctaacg cacacaggtt ggcaatcgac
     9481 tgctcaacat catgcgccac gatcccttca ttgccggtca cggcggtatc atccagcgcc
     9541 attaacaccg ctttccacgc agccgaagca ctggtcgaaa ccttcatcgc gcagctgttc
     9601 gacgcaccat cgcaaatcat gccgctgaca tcgccgatca tactgctgat cgccatcgag
     9661 atagtttcat aacgcccatc caccagccat gccatcccgg cggcggcccc cattgctgcg
     9721 gtcgttgcgg cacaaagtgc agacaaacgc ggtaactggt tatggatgta aattgcgctc
     9781 aaatgagaaa gcatcagcgc acgcgccagt cgttcatcat ccgctccgaa gtgttctgct
     9841 acaaccacca caggcattgt tgcggtgatc ccctggttac ccgagccgga gttactcata
     9901 gccggaagcg tagcgccgcc catacgcgca tcggatgccg cgctggtacg aatcacaatg
     9961 gatgaagaga gatctttcgc cagcaaaccg cgctcgcact gtttttccag cgtcgcgcca
    10021 atatgcagcc cccacttacc gctcaaacct tcctgcgata acgcacaatt tagcttcgcg
    10081 gaatcgagaa taaagcggat cgccgcaaac gggacttcat tgacgaactt caggatctca
    10141 gccagcgtcg ttctggaaag caccgtcagc ggagactctt gctcgccctc tgccacacac
    10201 tcctgctggg taaacaccac accattgtgc gtctcaatac gcacaatgtt ggtatgcccg
    10261 ccgacgatgg tgacacacgc ccacttctca ccgttccaga ctttggcgcg tgagaagagg
    10321 atttcattgc aaggttcctg gatcttaacg gagactttcc ccgccgccag cagtgctttg
    10381 gcatcggcaa ttgcctgcgc agttgcgtct ttcagcactt ccagcccggc gttggcattt
    10441 ccacctaacg cccccagcgc cgccgcaatc ggcagcccca ccattcccgt gccgggaacg
    10501 gtgacgccca gaccgttctt catcagattt ggcgaaaccc aggcttctac acgttcaacc
    10561 ggaccttcca gttctgccgc agcaaccgcc gccgccagcg ccagtgaaat cggttcagta
    10621 catcccagcg ccggttttac ttcctcctga acggcgagga tgtaacgctg ccataacgga
    10681 tttaaagtcg aatcaaacat attaaaaacc ttaaaatttc aggtaaatca agaaaatgcc
    10741 aggaacggag aaacacaaag caacaaacca gtgacgataa tcaggtacag agacatccct
    10801 ttgtatttgt gcaatgccgg tactttgtaa accagccacg ccgggatcag gcaccctacc
    10861 atgccgaaaa tcgggctaca gatagaggtg aagctcaaca ccggtgcgtt cagtacgata
    10921 gcgctccagg ccagcaaaat ggcgaaaatc atgatgccgc gctgaacaag attttcgtta
    10981 atcttctcgg caggcatctt gcgacgcagg atgttcatta cgatcccttg cgttgcttcg
    11041 cgaaagccta aatagacgcc aaagaatgca gtcatgacag caaagatatt gagaatgacg
    11101 ctgaccactt tcacccacgc tgcgccgtca ccgctaataa actgtgcggc aatcgccagc
    11161 gcggaaatat tctgctcgta ggcttttacc gcttcgtcat gccccatcgc cagcgtgaac
    11221 gacacggcgt agaaaaagac ggtgacaaac aaaatgccaa acgcgatatt cattgcccgc
    11281 aatgctttat gccgcgccac ttcaatagat ttttcccgcg agcgataaga gatcaccatc
    11341 gggcttaacg tctggataaa caaaatcgac gtcagggtaa acggcagcgt aataatggcg
    11401 tttttcacca gcagccccag cggcggtagc gaaccgacgt tatacagatg ccacattccc
    11461 accatcgaca cgcccagcgc cgcgaccacc agcagcttgg tcagcaccat gccggtcgaa
    11521 attttgaata acaatttctc gccgcgtgaa gagatcgcca ccagaatgca aatcagtacc
    11581 agaccataaa agggactgtc tgacagcaac ccttccgtca cgccgaaggt atgcagatag
    11641 gaagcactat cgttggtgat ggcggtggaa tagacgaaca tccaaatcac cagcattacg
    11701 aaatagagcg cacctaacag gatgccccag tttttaccta aataaccgct aatgacactc
    11761 gggtaatctt tacattctgg tgattctgcc agcgtattaa taaacaaccg ctgaaacaga
    11821 tacattgccg ggtaaccaat caccgatgag agcaaaaata cccacaatcc catcaaaccg
    11881 acctgcaccg ggagaaaaac aatccccgcg ccaatcgcca tcccgatact cataatcacc
    11941 cagccggtgt cagtactgtc gaatttgatc gcttctcgcc actcgctctc actcattcca
    12001 gcacgacccg ccggggtcga agcgtctgca atgacgcctt tattcgatgc gatttccata
    12061 atttctcgct caatattttg tagggcttat tatttttttc cgagccgcat caaggcgata
    12121 tgcggtctac gtttttgcag gcgagttatt agaataaaga aatgatacgc gcgaggtcag
    12181 agaaaatctt cacaatcaag actttgaaat aacggagatg gataagaatt ttctactaaa
    12241 ttaatcgcaa gagaatattc attctctatt tgcggcagat cacaaaaaaa aggtgcacat
    12301 ttgtgcaccc aaggatgaaa gctgacagca atgtcagccg cagaccactt taatggccag
    12361 tcctccgcgt gatgtttcgc ggtatttatc gttcatatct ttgccggttt catacatcgt
    12421 ctcgatcact ttatcgagtg aaacacgcgg tgccgaggtg cggcgcatcg ccatccacgc
    12481 ggcgtttact gctttcacgg cattaatggc attacgttca atgcacggga tttgtacctg
    12541 tccggcaacc ggatcgcagg tcagcccaag gttatgctcc atcgcgattt ccgccgcatt
    12601 gcatacctgc gccggactgc cgcccagtag ttcagttaac cctgccgccg ccattgaaca
    12661 ggccacgcca atctccccct gacagccgac ctccgcgcca gagatggagg cgttcatttt
    12721 atacagcgcg ccaatagccc ccgctgccag aaaatagcgg gcaattgacc gctcgtttac
    12781 cggacgacgg aacttatcgt aataagccag cactgccgga ataatgccgc acgcaccgtt
    12841 agtcggtgcc gttaccacgc gcccgccagc tgcgttttct tcactaaccg ccagcgcgta
    12901 catgttgatc cagtcgatga cattcatcgg atcgttagag atgttatcgc tagaaaccag
    12961 ctgacgacgc agcgctacgg cacgacgcgg cacattgagc ggaccaggca gcacgccttc
    13021 agtgttcatc ccgcgttcaa taccgtcatg catcacttgc cagatacggg caaatcctgc
    13081 gtcaatttcc gctttgctgc gcagcgccag ctcgttgtgc atcatcaggc cagatataga
    13141 caggccgttg taatcacaca ttttcagcag ttcacctgct gagtggaaat cgtaaggtac
    13201 ggacgtttcg acatcgtgcg acaggccgaa gtgttcttct tcgacaataa acccgccgcc
    13261 gacagagtaa taggttttac ttaatagcgc ttcctgtccc ttccaggcag tgatccgcat
    13321 tccgttctca tggcgaggca acatttcggg atggaagata atgttctttg ctacaggaaa
    13381 atcaacaata tgcgcaccag atgccactgg cagccgcccg ctgcgcgtta ctaactctat
    13441 aaatgcaggg atctcatcaa tgacaacatc ctgcggactg tttcctgcca gtcccatgat
    13501 aatggcgaca tccgtggcat ggcctttgcc cgtcagtgac aacgacccat acagatcgac
    13561 cacaatatgg ctcgtcgcgg ttaataagcc gctactttcc agccgatcaa taaaactttt
    13621 tccggcattc attggcccca cggtatgcga actggaggga ccaatcccaa ttttgaaaat
    13681 atcgaatgca ctaatcatat ccacaccctc ggattgccgt tcagtgaagt ggagcggaac
    13741 gaccttgcga ccgtcccgct cacgaggctt tacgcactac gtactgcgat agcttcaatt
    13801 tccagcttca catctttcgg caaacgcgca acctgcacac agctccgggt cgggtaggtc
    13861 gcctgatgct catcgaagaa ctgcttatag acttcattga tggtggcaaa atcattcaga
    13921 tcggtgatga acacggtcat cttgatgata tcgcccacgc tcagcccggc agcaaccacg
    13981 atcgctttga cgttttcgag gcttaaacac gcctgatctt gcacatcagc cgggatctca
    14041 ccggtctgcg ggcaaaccgg aatttgcccg gaggtgaaga ccatgctgcc taaatcaacg
    14101 ccctgaacat aagggccgat tgcgcctggg gcacgttgcg tttcgataat ctttttcata
    14161 catcctccgg cgtcagagcg cctgggtaaa agtacgtgaa ataacatcct gttgctgttc
    14221 acgggtcagt gcgttgaagc gcacggcgta gccagagaca cggattgtca ggttaggata
    14281 tttttccggg tgctcgatgg catccagcag catttcccga ttcattacgt tgacgttgag
    14341 gtgttgaccg ccttcgacat ccgcttcgtg gtggaaatac ccatccagca ggccgacaag
    14401 gttggtttta cgtactggat cttctttgcc cagcgccgca ggaacgattg agaaggtgta
    14461 cgagatccca tctttggcgt aggtgaacgg cagtttcgcc accgacgtca atgaggccac
    14521 ggcacctttg cggtcacgac catgcatcgg gttagcgccc ggcgcgaacg gtgttccggc
    14581 gcgacgaccg tccggcgtat taccggtttt ctggccgtac accacgttag aagtgatagt
    14641 cagaatcgac tgggtaggga cggcgttgcg ataggttggc agcgctttaa ttttcttcat
    14701 aaagcgttca accaggtcgc aggcaatgct gtctacgcgc tcgtcgttgt tgccgtactg
    14761 cggatattca ccgtcgattt caaagtccac cgccaggccg ttttcgtcac ggattggttt
    14821 cacgcgggca tatttgatgg cagacaggga gtccgtcgcc accgacaggc ccgcgatgcc
    14881 gcatgccata gtgcgataga catcacgatc gtgcagcgcc atcagcgaag cttcgtagct
    14941 gtacttgtcg tgcatgtagt gaatgatatt cagcgcgctg atgtactgca ccgccagcca
    15001 gtccatgaag tgatcgaggc tgtccatcac tttgtcgtaa tccagcacgt cgtccatcag
    15061 cggtgctgtt ttcggcccga cctgaatctt cagcttctcg tccaccccgc cgttaattgc
    15121 gtacagcagt gttttcgcca ggttagcgcg tgcaccaaag aactgcattt gcttaccaat
    15181 caccattggg ctgacgcaac aagcaattgc gtaatcgtcg ctgttgaagt cagtacgcat
    15241 cagatcgtcg ttttcatact gcaaggaaga ggtgacgatc gacacctgcg cggcatattt
    15301 tttgaaggca atcggtaatt cttccgacca aagaatggtc aggttaggtt ccggtgccgg
    15361 ccccatagtg tgcagggtgt gcaaatagcg gaaggagttt ttggtcacca gcgtacgacc
    15421 gtccagcccc atcccgccga tcacttccgt cgcccagatt gggtcgccgg agaacagcga
    15481 atcaaattcc ggcgtacgca agaagcgcac catacggatc ttcataatga agtgatcgat
    15541 cagttcctgt gcctgctgct cattgagtac gcctgcttta aagtcgcgct caatgtagat
    15601 atcgaggaac gacgccgtgc gccccagcga catcgcgccg ccgttctgtg atttcactgc
    15661 cgccagataa gcgaagtaga gccactgcac cgcttcctgc gcattctgcg ccgggcgaga
    15721 gatatcaaag ccatatttcg ccgccatttc ctgaatctgc aacagcgcat gacgatgctc
    15781 tgccagctcc tcacgcagac ggatggtggc ttccagatct tcgccttttt ccagacggga
    15841 ctggagatcg gcaaattgca gttcgcgttc acgcaccaga taactgatgc catacagcgc
    15901 tacgcggcga tagtcaccga taatgcgccc acggccatag ccatctggta aaccggtcag
    15961 cacgccagat ttacggcagc gcagcatatc cggtgagtaa acatcaaata cgccctggtt
    16021 atgggtttta cgcagatcgg taaacagata ttcaaattca ctgtccattt cacggccata
    16081 ggcgtggaat gaacttttaa tcatattaat gccaccgaac ggatgcagcg cgcgctttaa
    16141 tggcgcatcc gtttgcagac caacaatttt ttccagcggc tggttaatat atcccgcatc
    16201 atgagcggta attgtggtgg caatattggt atcgaaatca accggcgcgt gggttgcgtt
    16261 ttcgatacgg atgccttcca ttactttttc ccacaattcc gtggtggcag gcgtcgcttc
    16321 ggcgaggaaa gattcatcgc cttcatacgg tgtatagtta tgttgaataa aatcgcggac
    16381 attaatttcg tttttccagt ccgtaccttt aaagccaagc catgcgtcgg cgtacagctt
    16441 atcgctggta tcaatatcta tcttcatgaa aaataatctc tctacaatac ttcaactaaa
    16501 ttatgcaaat tctgcgggcg cgttaacttt gcctaaatga atggcatcca aagcaatcat
    16561 tttttcttcg ttagtcggaa taacggcaca aatgacacgc gcattttcac tggaaacaat
    16621 tcgctctcca aaagagttag agcgattatt catttctgta tcaatcacta cgcctaatac
    16681 agccaaatgt tccatgacca gacgacgaat taaacttgaa ttctctccta ttccgccggt
    16741 gaatataatt ccatccaggc gacgtaatga agctgcgtgt ccggcaatat gacgggcaat
    16801 tcggtgaaca aaggttttaa ttgccagttg cgcgcgttcg tgaccttcat gccaggcttt
    16861 ttccagaaca cgtaaatccg aagaaagacc ggaaatacct aataatcccg actctttatt
    16921 cactacgcgt tccaggtcac ccaggctctg gttggtttgg ctggcgaccc aggacatcgc
    16981 accaaagtcg acatcgccac tgcgggttcc catcatcaag ccttccagcg gcgtcattcc
    17041 cattgaagta tcaacactct gaccgttgcg aaccgcgcag attgacgcgc cattgccaag
    17101 atgcgccaca accaggccgg agtcatcttc cgccagattc agcagagaat gtgcgcgctg
    17161 ggaaacatag cggtgcgacg tgccgtggaa accataacgg cgtacaccta actcttcata
    17221 atatttccac ggcaggccgt ataaataagc ttccggagcc atcgtctggt ggaaactggt
    17281 atcaaatacc gccacctgag ttacgcccgg aaataattgc tgcgccgatt caataccact
    17341 taaattggcg taattatgca ggggtgccag tggagaaacg cgacggatat tatcaatgac
    17401 ttcatcggta ataatggcgg actcggtaaa aatactgccg ccgtgagcga tgcggtggcc
    17461 aattaaggcc acactgtcat ttaaattccg tttttccagt tcaaatgcaa ttgccttcaa
    17521 tgcaccttcg tagctgtggt gagccagcgg tgctggctct cccccattta cggataagaa
    17581 tgcattttcc gagttaatac cgtcggcaat acctgacatt aatacttcac agtcgctggc
    17641 atcgagcacg gaaaacttaa tcgaagacga accacagtta ataaccaaaa caaccggaaa
    17701 ttcattcatc tcttttctca tcctgagtta cggattaaaa cagtttgtat acgatgttca
    17761 ggatggtcag cagaccaatc acggtaacaa acacgttatc cagacgacca cggtatttcg
    17821 ccagagacgg cgctttacgg atggcataca tcggcaacag gcacagcagg gatgcgataa
    17881 tcggtgcgcc catggcttca atcaggtcga ggatgttcgg gttggcgtag gcaacaaccc
    17941 aggtggagcc catgatgaag atcatgctga tagtgttcag tttacccagc gacactttag
    18001 ttttgtcgcc tttataacca aacttcagga ccaggccatt cagaccttcc agcgttccca
    18061 gatagtgacc gaagaaagat ttgaagatag ccacgagtgc gatgatggaa gccgcatatt
    18121 ccagtgtaat cgcgaacgtt gttttggtac cggtcatgga cgcaaagtgg ttagccagat
    18181 aagaaagcac tggaatattc tgcgctttgg cttccgccat gttggccgga gacagcgtaa
    18241 ataggcagct aaaggcaaag aacatcacca ctgcaaccat cagcatgctg gcacgagaaa
    18301 tgatttggga acatttacgt tcggtgaagt cgcgaccgaa gtctttctca tactcttcac
    18361 gtttagaaac cacgaaggaa gagacgattg gcgagaaatt aaaggagaaa accatgatgg
    18421 aaatccccag ccagacggtg atcaggatac cgtcatgacc ggttaacgac agcgaaccga
    18481 ggtcaacctg gtcgataact gcagagttcc agtaagggat cagcgacaga gaaatcagca
    18541 ccaggctggc gataaacggc cataccaggt agctcatcac tttaaccatc agatccttac
    18601 caaaccagat gacgaaagcc atcagcagca gcaggaacag cgccacaaag ccgcgattca
    18661 gcggtgcaaa gccgagctgg ttttcccaga acgtcataaa ggtattggta atagtaacgc
    18721 cataaatcca cagcagtggg caaatcgcga agaagtacag gaacgtgata accacgccgc
    18781 cagttttacc aaaatgctct tccaccgttt ccgtaatgtt gccggaaggg ttagagccag
    18841 aaagacatag acgcgccagc gcccggtggc aataaaatgc gatggggtat gccaatacca
    18901 acatcagaag aatcgggatc agtccgccaa aacctgcgcg gatagggaag aacagcaccc
    18961 cggcgccgat tgccgtacca aacaagccta acgtccatgt ggtatctgat ttacgccagg
    19021 acgattgttt tgtctggctg gatacaatgc tatctgaagt actcatatcc tatcctcaac
    19081 gaattaatta agcgtcaacg aaaccggtga tttgagagac gcgagaaaga tcgatattgc
    19141 cgccggaaat aatactgacg gtttttctgt tttgaatata ttggtctaat ttaccgctta
    19201 ataatgcagc acatgccaga gcgcctgcgc cttcggtgac gactttattg cgctgaatta
    19261 aggcaatcat actgtttctg atttcgtctt cgctgaccag cacgatgtca tcgactaatt
    19321 cacgaacgat ttcgtaagtt aaattacccg ggcgggagac atcacaacca tccgccaggg
    19381 tgccggtagt tcggtgcgtg gttatttctc cggagtggaa agaagccgcc atgccgtgaa
    19441 cgttttcaga ctgtacgcca ataacacgaa tggtcgggtt aatagattta attgccaccg
    19501 caataccagc aattaaaccg ccaccaccaa ttggcacaat cacgttatcg acatcataga
    19561 gatcttccat aatttccaga ccaatcgttc cctggccagc aatcactttc ggatcatcgt
    19621 aaggtgggat aaaaatacgg ccttccattt cgacaatttc gctcacttta gcgatagtgt
    19681 cgttgaagtt atcaccatgc agaacgactt ctgcggagta gtcgcacgtt gccgctactt
    19741 tggattttgg cgcacctttt ggcatcacca ctttaccgtc gatacccagc atcgcgcagg
    19801 agagcgaaac cccttgcgca tggttgcccg cagaacaggc caccacgcct ttgcgttttt
    19861 ccgcatcggt cagtgaactt aatttattaa atgcgccacg aattttaaat gaacccgtac
    19921 gctgcatatt ttcaaacttc aggaatattt cacctttgca acgttcacta aaatagttgg
    19981 agcgaggcat gcctgtttta taaattcgcc cagccagtcg ttgtttcgct tcaataatat
    20041 catcaatagc aatcggcaga tcgtatgtaa tatgcattat aaaacctctt cgcctgaatt
    20101 aaatatgtag gtaaccgtaa ccgacacctg caaaacagac aggtggatta tttatgttta
    20161 aataaaaaca aataactaac caacttctat taattgcctt cgtctacacc cattataaga
    20221 tgaatactct ttggctaatt ccaccaaaac cgatgctgct tttttaatac gataattttt
    20281 cgaccatacc gcggcatatt gtgccacagg taatgtttct tcaaccggaa tagtaataaa
    20341 ttgattagaa ccaaaaggtg acgtcatatc acaaggaatt acagttaaga aatcagcatt
    20401 gagaacaaga ttataaattg tcacgactga gtcggtttta acgatgtttt caatactgat
    20461 gccatttctt tgtaacgtag taagcagttc gctgtagtac cccatattag tttgtggcaa
    20521 cacccactgt tcgttcttca acgactccag cgtggtggtg ccggtgcatg ttcgggactt
    20581 actggctacc agcacaaact cggactcgaa cagcggctca acatgtaaat cctgaagctt
    20641 catttctgca cttaatgtac caatcgcaaa atccagtcga ccgtcgcgga ttgccggtaa
    20701 gaacgaagac agttgcgctt catacataga aacctgcgct ttcgggaaca cctctttgaa
    20761 cttgttgatc atccctgaca taaaagtaaa accaatcaat gaaggaaaac caaatgagac
    20821 ttccaccacc gcctcagaag acataccgct tatctcatta accatatttt tcatttcacg
    20881 ggtaatggat tcggaacggg agagtaacaa ttgaccggca ggtgttaatg ttacaccggt
    20941 atttttccgc accactaatt ccacaccaaa ataatcttca atatcgttaa tgattttact
    21001 gacggccggt tgagttaacc ctaattcttt tgcagccgag ccgatagagc cacttctaat
    21061 gacttcctga aagactacca ggtgctgcgt tttcggaaga agaatagtgc tcataacgac
    21121 ctacgttaat tacctcattg acggcatgaa gtgtatcaaa atgaaatgaa caggatatgt
    21181 gcgaccactc acaaattaac tttcaatact ttccagggta tcgtcattac aattaaatac
    21241 ctttaaatat caacaagtta aagcataaaa atcagcataa accctgattt ttgtcaaaaa
    21301 aaatgacggg gataaccata ttttatggcg ataacatcat tcgttatgtg gttaatacaa
    21361 aaaaggggct gagaccatat ttcaagcaat taccggataa ttaccaggca aaatttgagt
    21421 taaatttaat gagcaagttc tcaaatttta ttataaataa accattttca gcgataaata
    21481 ctgcggcacg tcacattttt tcacgctatt tgttggagaa caaacattta ttttatcaat
    21541 attttaaaat ttcgaataca ggtattgatc atctcgaaca attgattaac gtcaactttt
    21601 tctcttctga caggacgtca ttttgtgaat gcaatcgttt tccataaatt cttctcccct
    21661 cataggcgac gaatagcatt ttgtgttgag gatcacaaaa cgaataattg ctgatcgccg
    21721 cgataaggtc agacaaagac aacaagggaa attttcacag agcttttgat cgtcgtaggc
    21781 cacagaatgt tgcttcgttt actgtaacgc cgggtaaatg agcgtttttt gatagtgcga
    21841 aagaaccctg cgaggaaaat aagcatctat tattgtttgt catgacgaca aggcaggcat
    21901 caatgaaagg cttcccaatt gcacatattt ttcacccttc aatcccgcca atgcacgcag
    21961 tggttaacaa tcacaataga aatattgatt attggacggt aaaaagaaag tttg
//
LOCUS       DAJVQG010000063        21857 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJVQG010000063 DAJVQG010000000
VERSION     DAJVQG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21857
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.063"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            270..1388
                     /locus_tag="OQM83_002120"
     CDS             270..1388
                     /locus_tag="OQM83_002120"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCS1520385.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            1454..1702
                     /locus_tag="OQM83_002121"
                     /pseudo
     CDS             1454..1702
                     /locus_tag="OQM83_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            1767..2135
                     /locus_tag="OQM83_002122"
     CDS             1767..2135
                     /locus_tag="OQM83_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCS1520386.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2229..2882
                     /gene="nfsB"
                     /locus_tag="OQM83_002123"
     CDS             2229..2882
                     /gene="nfsB"
                     /locus_tag="OQM83_002123"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCS1520387.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            2990..4237
                     /gene="ybdG"
                     /locus_tag="OQM83_002124"
     CDS             2990..4237
                     /gene="ybdG"
                     /locus_tag="OQM83_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCS1520388.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4305..5681)
                     /gene="pheP"
                     /locus_tag="OQM83_002125"
     CDS             complement(4305..5681)
                     /gene="pheP"
                     /locus_tag="OQM83_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCS1520389.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5783..8926)
                     /gene="cusA"
                     /locus_tag="OQM83_002126"
     CDS             complement(5783..8926)
                     /gene="cusA"
                     /locus_tag="OQM83_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCS1520390.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKARENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(8938..10161)
                     /gene="cusB"
                     /locus_tag="OQM83_002127"
     CDS             complement(8938..10161)
                     /gene="cusB"
                     /locus_tag="OQM83_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCS1520391.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(10177..10509)
                     /gene="cusF"
                     /locus_tag="OQM83_002128"
     CDS             complement(10177..10509)
                     /gene="cusF"
                     /locus_tag="OQM83_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCS1520392.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(10533..11915)
                     /gene="cusC"
                     /locus_tag="OQM83_002129"
     CDS             complement(10533..11915)
                     /gene="cusC"
                     /locus_tag="OQM83_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCS1520393.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            12072..12755
                     /gene="cusR"
                     /locus_tag="OQM83_002130"
     CDS             12072..12755
                     /gene="cusR"
                     /locus_tag="OQM83_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCS1520394.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            12745..14193
                     /gene="cusS"
                     /locus_tag="OQM83_002131"
     CDS             12745..14193
                     /gene="cusS"
                     /locus_tag="OQM83_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCS1520395.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            14930..16831
                     /locus_tag="OQM83_002132"
     CDS             14930..16831
                     /locus_tag="OQM83_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCS1520396.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRPPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDSIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
     gene            16859..17320
                     /locus_tag="OQM83_002133"
     CDS             16859..17320
                     /locus_tag="OQM83_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HCS1520397.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            17340..21751
                     /locus_tag="OQM83_002134"
                     /pseudo
     CDS             17340..21751
                     /locus_tag="OQM83_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR/RHS domain-containing protein"
BASE COUNT         5251 a         5863 c         5793 g         4950 t
ORIGIN      
        1 caaaatggct gtttatttat acagtaaatc ctgtttcgga cttctcactc ctcatttaat
       61 caactgaagc tgagcccgtt ttttctgcat gcctcaatcg tcaaaattct tcgccatcag
      121 ccagctctgc cataacttgc cacattgaaa aagtagccaa attgcttcag tcaccatcat
      181 caaaaaaaga aacattccag aacttccgca tctcgcccag ccgctccgac ttctatactg
      241 aatagaaaac gccaacataa gagaaaccta tgccattacc cgattttcat gtttctgaac
      301 cttttaccct cggtattgaa ctggaaatgc aggtggttaa tccgccgggc tatgacttaa
      361 gccaggactc ttcaatgctg attgacgcgg ttaaaaataa gatcacggcc ggagaggtaa
      421 agcacgatat caccgaaagt atgctggagc tggcgacgga tgtttgccgt gatatcaacc
      481 aggctgccgg gcaattttca gcgatgcaga aagtcgtatt gcaggcagcc gcagaccatc
      541 atctggaaat ttgcggcggt ggcacacacc cgtttcagaa atggcagcgt caggaggtat
      601 gcgataacga gcgctatcaa cgaacgctgg aaaactttgg ctatctcatc cagcaggcga
      661 ccgtttttgg tcagcatgtc catgttggct gcgccagtgg cgatgacgcc atttatttgc
      721 tgcacggctt gtcacggttt gtgccgcact ttatcgccct ttccgccgcg tcgccatata
      781 tgcaaggaac ggatacgcgt tttgcctcct cacgaccgaa tattttttcc gcctttcccg
      841 ataatggccc gatgccgtgg gtcagtaact ggcaacaatt tgaagccctg tttcgctgtt
      901 tgagttacac cacgatgatc gacagcatta aagatctgca ctgggatatt cgccccagtc
      961 ctcattttgg cacggtggag gttcgggtga tggatacccc gttaaccctt agccacgcgg
     1021 taaatatggc gggattaatt caggctaccg cccactggtt actgacagaa cgcccgttca
     1081 aacatcagga gaaagattac ctgctgtata aattcaaccg tttccaggcc tgtcgctatg
     1141 ggctggaagg cgtcattacc gatccgcaca ctggcgatcg tcgaccgcta acggaagaca
     1201 ccttgcgatt gctggaaaaa atcgcccctt ccgcacataa aattggtgca tcgagcgcga
     1261 ttgaggccct gcatcgccag gtcgtcagcg gtctgaatga agcgcagctg atgcgcgatt
     1321 tcgtcgccga tggtggctcg ctgattgggc tggtgaaaaa gcattgtgag atctgggccg
     1381 atgactaagt agggaattgc cttacagaag aatatccctg acaatggtgt tttaacttac
     1441 atttaacaat gttatgaaac accctttaga aaccttgact accgcagcag gcattttgct
     1501 gatggctttc ctctcttgcc tgctgctgcc cgcccccgca ctggggctga cgctggcaca
     1561 aaaactggtg accatgttcc atctgatgga tcttagctag ctttacactt tattgttttg
     1621 tctgtggttt ttagtgctgg gcgctattga gtattttgtt ctgcgcttta tctggcgacg
     1681 ctggttctcg ctggcggatt aaacgtcgat tatcgccgta cttcgcggca tactttgctt
     1741 attctccttc gcctaaagga acgtttatgg ataagcaatc actgcacgaa acggcgaaac
     1801 gcctggccct tgagttaccc tttgtcgagc tttgctggcc ttttggcccg gagttcgatg
     1861 tttttaaaat tggcggcaaa atttttatgc tgtcgtcgga gctgcgcggc gtccccttta
     1921 tcaatctgaa gtccgatcca caaaaatccc tgttaaatca gcagatatac ccgagcatta
     1981 agccagggta tcacatgaat aaaaagcact ggatttcggt gtatcccggc gaggaaatct
     2041 ccgaagcgtt acttcgcgat ctgatcaacg attcgtggaa tctggtggtt gatggtctgg
     2101 ctaaacgcga tcaaaaaaga gtgcgtccag gctaaagcgg aaatctatag cgcatttttc
     2161 tcgcttacca tttctcgttg aaccttgtaa tctgctggca cgcaaaatta ctttcacatg
     2221 gagtctttat ggatatcatt tctgtcgcct taaagcgtca ttccactaag gcatttgatg
     2281 ccagtaaaaa acttaccccg gaacaggccg agcagatcaa aactctcctg caatacagcc
     2341 catccagcac caactcccag ccgtggcatt ttattgttgc cagcacggaa gaaggtaaag
     2401 cgcgtgttgc caaatccgct gccggtaatt atgtgttcaa cgaacgtaaa atacttgatg
     2461 cctcgcacgt cgtggtgttc tgtgcaaaaa ccgcgatgga cgatgcctgg ctgaagctgg
     2521 ttgttgacca ggaagatgct gatggccgct ttgccacgcc ggaagcgaaa gccgcgaacg
     2581 ataaaggtcg caagttcttc gctgatatgc accgtaaaga tctgcatgat gatgcagagt
     2641 ggatggcaaa acaggtttat ctcaacgtcg gtaacttcct gctcggcgtg gcggctctgg
     2701 gtctggacgc ggtacccatc gaaggttttg acgccgccat cctcgatgca gaatttggtc
     2761 tgaaagagaa aggctacacc agtctggtgg ttgttccggt aggtcatcac agcgttgaag
     2821 attttaacgc tacgctgccg aaatctcgtc tgccgcaaaa catcacctta accgaagtgt
     2881 aattctctct tgccgggcat ctgcccggct atttcccctc agattctcct gatttgcata
     2941 accctgtttc agccgtcatc ataggctgct gttgtataaa ggagacgtta tgcaggattt
     3001 aatatcccag gttgaagatt tagcgggtat tgagatcgat cacaccacct cgatggtgat
     3061 gattttcggt attatttttc tgaccgccgt cgtggtgcat attattttgc attgggtggt
     3121 actgcggacc ttcgaaaaac gagccatcgc cagttcacgg ctttggttgc aaatcataac
     3181 ccagaataaa ctcttccacc gtttagcttt taccctgcag gggattatcg tcaatattca
     3241 ggcggtattc tggctgcaaa aaggcaccga agcggcagat attctgacta cctgcgcgca
     3301 gttgtggatc atgatgtatg cgctgctttc agtcttctcg ttgctggatg ttattttgaa
     3361 tctggcgcag aaattcccgg cggcatctca gttaccgctg aaagggatat ttcaggggat
     3421 taaactgatc ggcgcgatcc tggtcggcat tttgatgatc tcgctgctga ttggtcagtc
     3481 gccagcgatt ctgatcagcg gtcttggtgc aatggctgcc gtgctgatgt tggtatttaa
     3541 agatccgatt cttggtctgg tggccggtat tcagctttcc gcgaacgata tgctgaaact
     3601 gggcgactgg ctggagatgc cgaaatacgg cgcagatggc gcggtgatcg atattgggtt
     3661 aaccaccgtc aaagtgcgta actgggacaa taccattacc actattccca cctggtctct
     3721 ggtttctgac tcctttaaaa actggagcgg gatgtcagca tctggcgggc gacgtattaa
     3781 gcgcagtatc agtattgatg tcaccagtat tcgttttctt gatgaagacg aaatgcaacg
     3841 tctgaataaa gcgcatttgt taaagcctta tttaaccagc cgccatcagg aaattaatga
     3901 gtggaatcgc cagcaaggtt ctacggagtc ggtattaaac ctgcgccgaa tgaccaatat
     3961 tggaaccttt cgtgcctatc tgaacgaata tctgcgtaac catccgcgga ttcgtaaaga
     4021 tatgacctta atggtacgcc aactggctcc aggtgataac ggtttaccgc tcgagatcta
     4081 cgcgtttact aacaccgtgg tgtggctgga atatgaaagc attcaggctg atatattcga
     4141 tcacatattt gcgattgtcg aagagtttgg tctgcgactt catcagtcgc caaccggcaa
     4201 tgatattcgc tctctggcgg gtgcatttaa gcagtaatta aaaaaaccgc tctcatcgaa
     4261 tggatgagag cggacatcgt tttgtcggat gtagcgtgaa tgccttattt ccgacgcagc
     4321 gttttaaatg ccacaaacag gaatacaatc cacaccggca gcaggatcgc tgacaagcgc
     4381 atatcatcca tcgtgcacat cagcagcaaa atcatgccga ggaaggcaat gcagagatag
     4441 tttccgaacg gatagagcag cgccttaaac tgtgtttcac gcccctgacg tcgcatcgct
     4501 gcacgaaaac gcagatgcgc cagacagatc ataatccagt tcaacagcag cgttgctacc
     4561 accagcgcca tcagcagacc aaacgctttt tgcggcagca gatagttgat taacaccacc
     4621 agcgaagtga tcgctccgga aagcatcagc gagttaatcg gcacaccgcg acggctgacg
     4681 cgagtcaaaa acttcggcgc attaccctgc acagaaaggc caaacagcat gcggctgtta
     4741 gagtaaaccc cgctgttata cactgacagc gatgctacca gaatgacgaa gttcagcgca
     4801 gaagctacca cgttgctgtc gagattatgg aaaatcatca caaacgggct actgttggat
     4861 ttcacttcca cccacggata gagcgccagt aaaaccacca gtgaaccgat gtaaaacagc
     4921 aggatgcgat acaccacctg atttaccgct tttggaatgc ttttatccgg atcgcgcgct
     4981 tcagcggcag taatcccaat cagctccaga ccgccgaagg agaacataat taccgccagc
     5041 gacaaaatca gcccattcca gccggtggcg aagaaaccac cgtagcgcca gaggttgtcg
     5101 atactggctt tctcgccgcc gtgaccagaa aacagcagcc acaggccaaa gccgatcata
     5161 ccgatgattg ccagcacttt aatcaacgca aaccagaact cggtttcgcc atataagcgc
     5221 acgttcacca ggttaacggc gttgatgata ataaagaagg cggcagccca aatccacgtt
     5281 ggaacatccg ggaaccagta ctgcatatag atgcccgcag cggtcagctc tgccattccc
     5341 accagcacga acattaccca gtagttccag ccagagagga agcccgcaaa cggtccccag
     5401 tatttatagg caaagtgggc aaatgaaccg gataccggct cttcgaccac catctcgcca
     5461 agctggcgca taatcaggaa agcgatgatc ccggcgacgc cgtaacccag caatacagcc
     5521 ggacccgcca tctgaatcgc cggaccaatg ccaagaaaca gaccagtacc aattgcgcca
     5581 cccagcgcaa tcagttgaat atgacggtta tgtaatccgc gatgaagcgt cggctcttga
     5641 ttcgacgcag tatcttccga tacggttgac gcgtttttca cgcctttccc ctgtgtgtct
     5701 tttttgttga ggggcacctt ttaacattta gtgcccatcg tcgcaagaca caatccacac
     5761 ggttaaacgg ggtatcctgc ttttatttcc gtacccgatg tcggtgcagc cacatcagct
     5821 tatacgccgc cgggataata aacagcgaca gcaaaggtgc ggtgatcatg ccgccaatca
     5881 tcggtgcggc aatccggctc atcacctctg aaccagcccc cgttccccac agaatcggca
     5941 gcagaccggc gataatcacc gccaccgtca tcgctttcgg gcgcacgcgc agtaccgcgc
     6001 cgtgatacaa cgcctcatcc agcttctgct cactgaatgt ttgcggatta ttcaacgacg
     6061 gctcggcctc tatggcgtga cgtaaataca tcagcatcac cacgccaaat tcggcggcga
     6121 ccccggcaag ggcaataaaa ccggtgcccg ttgccacaga aagatgaaag cccatccacc
     6181 acagcaacca gatgccgccc accagcgcaa acggtacgct gctgataatc agcaacgctt
     6241 cgcccacccg acggaatgcc agatacaaca gcacgaagat gatcatcagt gtcatcggca
     6301 ccatgagttt cagcttatgg ttggcgcgct ccagtagctc gaactgcccg gagaatgcca
     6361 cgctggtgcc cggttttaac tgcacttttt cagctatcgc tttttgcaaa tcgtgaacca
     6421 ccgacaccat gtcacgatcg cgggcatcaa tataaatcca gctcgtcggg cgcgcattct
     6481 cggttttcag catcgacggt ccggtagaga ctttaacgtc ggccacgtct gccagggtga
     6541 tttgctgctt catcggcgtc aggatcggta gctggcgcag tgcctgcggg ctatcgcgcc
     6601 agctttgcgg ataacgcaga ttaatgggat aacgggcaat cccttccacc gtttcgccaa
     6661 ccatcgcccc gcccaccgca gaagtcacaa acaactgcac atccgccacc gtcataccgt
     6721 aacgcgcggc tttttcacgg ttaatctcaa cgttgatata gcgcccacct tccagacgct
     6781 cggctagagc agaagccacg cttggcaccg ttcgcgctac ttcttcaatt tgctcagcca
     6841 tcgcgtcgat atccgccagc acagtgccgg aaactttaat gccgatgggg cttttaatgc
     6901 cggttgagag catatcgata cggttacgaa ttggcggcac ccacagattc gccagccccg
     6961 gcagccgcac ggtgttatcc agttcctcaa tgattttgtc catcgtcatg ccaggccgcc
     7021 actgatcctg cggcttaagc tggatggtcg tttcaaccat ctcgagcgga gcagaatcgg
     7081 tggcggtttc cgctttcccg gttttgccaa atacccgcgc cacttcaggt acgctcataa
     7141 ttagcttgtc ggttttttgc agcatactcg ccgcctctgc tgcggaaatc cccggcagcg
     7201 tcgatggcat atacaacaag tcgccttcat tgatctgcgg taaaaattcc ccgccaactt
     7261 tattgagcgg ccagagaacc gtcagcaccg aaagcgctgc caccaacagc gtggttttcg
     7321 gccagtgcag tactttcagc aatagaggat gataaacacg aatcaaaaag cgattgagcg
     7381 ggttactgct ttccggcgga attttgccac ggatccagta ccccatcagg atagggatca
     7441 ctacgatcgc cagcagcgcc gcgcccgcca tcgcataggt tttagtgaac gccaacgggc
     7501 caaacagacg cccttcctgc ccttccaggg tgaagatcgg gataaacgac aacgtgataa
     7561 tcagcagact gataaatagc gccggaccca cttcaacaga tgcatcggtg atcacctgcc
     7621 agcgcgtttt attatccagc gtggcgtcag gatgctggtg ctgccactct tccagccgtt
     7681 tatgtgcatt ttcgatcatg acgatagcgg catcgaccat cgccccgacg gcaatcgcaa
     7741 tgccgcccaa cgacataata ttggcattca gtccctggaa gtgcatgaca ataaaagcaa
     7801 tacacaaccc cagcggcaac gaaataatcg ccaccagcgc cgagcgcaca tgccagagaa
     7861 acagcgcaca gactaccgcc accacgataa actcttccag caacttgccg ctgaggttgt
     7921 cgatggcgcg gtcaatgagc tggctgcgat cgtatgttgt aactatctcc acgccttccg
     7981 gcagactact tttcagcgtt tccagtttgt ccttcacgga ggcgatcact tctcgggcgt
     8041 ttttgccgga gcgcaggatc accaccccgc ccgccacttc gccttcgcca ttcagttcgg
     8101 caatgccccg gcgcatctcc gggccaatct ggaccttcgc aacatcacgc agataaacgg
     8161 gcacgccatt ttctctggct tttaaaacga tgtgattaaa gtcgtcgagc gtttgcagat
     8221 agccgctggc gcgcaccata tattccgctt ccgccagttc gatcgacgaa ccgcccgctt
     8281 cctggtttga agcatccagc gcgcttttta cttcggctag actgatgcca tattgcgcca
     8341 ggcgctgggg atcgataacc acctgatact ctttcaccac gccgcccacc gacgccactt
     8401 ccgcaacgtc agggatggtt tttagctcat atttgagaaa ccagtcctgc aatgagcgta
     8461 aatcggccag atcgtgctta ccgctgcgat ccaccagtgc atattcatag atccagccaa
     8521 cacccgtggc atctggtccc agctcggcgc tgactcccgc aggcagctta ccctgtacct
     8581 ggttgaggta ctccagcacc cgcgaacgcg cccagtacgg atcggtacca tcttcgaaaa
     8641 tgacatacac ataagagtct ccaaactgcg agaaaccgcg cacagtcttc gcgccaggca
     8701 ccgacaacat ggtggtggtt agcggataag tcacctgatt ttcaacgatt tgcggtgcct
     8761 gaccgggata gctggtttta ataatcacct gcacatcgga gagatccggc agcgcatcca
     8821 ctggcgtgtt aatgatggtc caggtgcccc agatgctcag aaacaacgcg cccatcagca
     8881 ccaaaaaacg gttcgccacc gagcgacgaa taatccattc aatcattggt tattccctca
     8941 atgcgcattg gtagcacttt cagagcgcat ccgctccagt gcgccagaaa tattggcttc
     9001 ggaatcaatc aggaacaggc cgctggaaac caccttttca ccttccgcca gaccagagtg
     9061 taacgcggtg acgccttgcg atgcctggaa aacggcaacg cgtttcggta caaagcgtcc
     9121 gtcggcatca acggtaatca cccgctgttc gttgccggta tcaatcagcg cctgtgacgg
     9181 aatgagcagc atcggttcgc tggcggtgtt gagttgcaac caggcgttca tgcccggttt
     9241 tagcgcctcg tcggcgttgt cgacttccag acgcagctgc agcgtgcggg tcgcggcatc
     9301 cacgccaggt agcagcgtcc atttgcggat ggtgagtgtt ttatccggtc gcgccggaac
     9361 ggtgagcgta aactgcgagg catctttcac cagccaggcg atagactccg ggatcgcagc
     9421 agtgacccac accgggtcca taccctgaat tttcgctacc acgttatctt tggcgatatt
     9481 cattcccgcg cgcagatcaa acgcggtgat cacgccatca atgggtgctt tgagcgtaaa
     9541 gcgagtctgg attttttgcg tggcgatcag gcggcgaata tccgcctccg gcattcccgc
     9601 cagtcgcagc cgctcaagaa tgccttcagt ctgggtcgcc gtaccgccgg tttcgcgcag
     9661 cagtaaatac tcactctgcg cttccaccca gtcaggaatg gtcaggtcga gaagcggtgt
     9721 gcccttttgt actttatcgc ccacggtaag cggatacacc ttgtcgataa acccggcagc
     9781 gcgagcctgc acaatggcat actgatactc gttgtaactg acattcgccg ggaaactctg
     9841 ggcaaaagtc agcggtccgc gcgtgacggt agccgttttc acccccagat tctgcgtctg
     9901 agtcgggtca atgcgcacac cagacgcaga actctcttca tcggcatatt tcggcaccag
     9961 atccatatcc ataaacggcg atttacctgg tttatcgaac cgcgtattgg ggtacatcgg
    10021 gtcgtaccag aataagattt tacgttctgc ggtcgacgtt ttttctgcgg gtggttcagc
    10081 ctttgcaaac caggtaaaac ctgccgcaga aataataccg cccgcgatca tgctgccgat
    10141 aataagcgcg atttttttca tctcattaaa cctgggttac tggctgactt taatatcctg
    10201 taataaagaa aggttgccct gctggacaaa attaaacgcc actttgtcgc cggttttaat
    10261 ttcactcatt ttcgtctgcg gggtgatggt aaagcgcata gtcatctccg gccagttcac
    10321 ggcagcaatc ggatcgtgat ggatggtgat ttttttgctt tccagatcaa taccctttac
    10381 cacgccagtg gcgctaataa cctgcggttg tacttcgctc atggtttcat gatgatgttc
    10441 gttagcctgg gcattaaagc caataacggt aaacagactg aacattgcga cttgcagtgc
    10501 ttttttcatt tattctctcc tggagttaaa aattattgct gccaaccgcc ccctaaggcg
    10561 gtatacaaag aaatttcgtt aacctgacgg gcataattca ggtcgagtaa agtttgtcgg
    10621 gttgcaaata aagaacgctc ggcatccagc acttccagat aacttaccgc gccgtgctga
    10681 tataatgccc gcgcccgttg caaagtaatt tgcagcgacg ccagataacg ctgctgggcg
    10741 ctgatttgat cgttcaggct ttgacgtaat gccagcgcat ctgccacttc tttaaaggcg
    10801 ttctggattt tctgttcata attcactacc gactgctgct ggcgaatttc ggcgatatcc
    10861 agattggcct ggttgcgtcc ggcattaaaa atggggatct caattttagg aataaaattc
    10921 cacatcccgc tgctagcgtt aaataatgaa gacagatcgc tgctggcggt tgatattccg
    10981 ctggtcaggc tgatagacgg gaaaaatgcc gcacgcgcag caccaatatt agcattagcc
    11041 gccattaacg cgtgttcagc ttccataata tccgggcgct gcaataagat ttgcgacgac
    11101 aagcccgccg gtaatttaac gctttgcagg ctgccgctgt ttactgtctg cgcttgcggc
    11161 agtttgccgt agcttcccaa taacagttgc aatgcattat tcgcctgcgc cagttccccc
    11221 tgacgtttag cgatgtcact gcgggtactt tctatcaccc cgcgagcctg ttccagcgcc
    11281 agaacattgc tgctaccggt caacagttgt ttttcgacaa acgcatatga ctgctgataa
    11341 ttacgcagcg tttcttcggc tatttgcaat tgcgcatacg ccagttgctg attgaaatag
    11401 ctttgcgcga cattagaaac cagcagaata tgcaccgcgc gctgagcttc ctcagtggct
    11461 aaataatttt gtcgctcggc ttcgctcatg ttctttaagc gaccgaaaaa atcgaggtca
    11521 aagctggcgt taaggccggt cgaaaactcc cgtgtcgtgg cggtattccc tttaagatta
    11581 ccgctccagc tgccgctgcc ctcgccattg agctgtgggt agcggtcggc atcggtcaga
    11641 cgatattgcg cccgtgcctc ctgcactttc agcgtcgcca tgcgcaaatc ccggttattc
    11701 accagcgcct cgctaatcag cgtcttcacc tgattatcaa caaaaaaggt gcgccagccc
    11761 acgttctggt agttatctgc tgcgttaacc aagccgttct ggctgagtga gaactgctgc
    11821 ggcacgggca ttgccgtacg ctgataatcc ggtgccagtg aacaaccggt tagcgcaagg
    11881 gccacacaaa atggcagaag tttacaagga gacataggct cataatttct ggtgatttta
    11941 taccgccaac tttactcgcc aggctctgat tttccggtga caggaaaatg acaaaattgt
    12001 cattttgcca ataagcgatt gccatctgat cccgctactc tagaattgcc cgggcaacat
    12061 gcggaggaaa aatgaaactg ttgattgtcg aagatgaaaa gaaaaccgga gaatacttga
    12121 ccaaagggtt aaccgaagcc ggttttgtgg tcgatttggc cgacaacggg ctgaatggct
    12181 accatctggc gatgaccggt gattatgacc tgataatcct cgatattatg ctgccggacg
    12241 tgaacggctg ggatatcgtg cgcatgttgc gctccgccaa taaagggatg ccgattctgt
    12301 tgcttaccgc gcttggcacc attgaacatc gcgtcaaggg gctggagttg ggggccgatg
    12361 actatctggt gaagccgttc gcttttgctg aactgctggc gcgggtgcgc actctcctgc
    12421 ggcgcggggc ggcggtgatt atcgaaagtc agtttcaggt tgccgatctg atggtcgatc
    12481 tcgtcagccg caaagtcacc cgcagcggca cgcgcatcac tctgaccagt aaagagttta
    12541 ctctgctgga gttttttctc cgccatcagg gcgaagtgct gccccgctcg cttatcgcct
    12601 cgcaggtgtg ggacatgaat ttcgacagcg acactaacgc cattgatgtg gcggtgaagc
    12661 ggctacgcgg caaaatcgac aacgactttg agccgaagct gattcagacc gtgcgcggcg
    12721 tgggctatat gcttgaggtg ccggatggtc agtaagccat ttcagcgccc gttttcgctg
    12781 gcaacccgtc tgaccttttt tatcagcctg gccaccatcg cggcgttttt cgcctttgca
    12841 tggatcatga tccactcagt gaaagtgcat tttgccgagc aggatattaa tgatttaaaa
    12901 gaaattagcg ccacccttga acgggtactt aatcatcctg acgagacgca agcccgacgc
    12961 ttaatgacgc tggaagatat cgtcagtggt tattccaacg tgttgatttc cctggcagat
    13021 agccacggca aaacggtgta tcactccccc ggtgcgccgg atatccgcga gtttacgcgt
    13081 gacgccatac ccgataaaga cgctcagggt ggcgaggtgt atctcctttc cggcccgacg
    13141 attatgatgc caggccacgg tcacgggcat atggaacaca gcaactggcg gatgattaac
    13201 ttgccggttg gcccgttggt ggacggcaaa ccgatttata cgctctacat cgcgctttcg
    13261 atcgattttc atcttcatta cataaatgat ttgatgaata aacttattat gaccgcatct
    13321 gtcatcagca tcctgatcgt ctttatcgtg ctattggcgg tacataaagg tcacgcgccg
    13381 atccgcagcg tcagccgtca aatccagaat atcacctcga aagatctcga cgttcgcctt
    13441 gacccgcaga ccgagcccat tgagctggaa cagctggtac tgtcgttcaa ccatatgatc
    13501 gagcgtattg aggatgtctt tacccgccag tccaatttct cagcggatat cgctcacgaa
    13561 attcgcacgc cgattaccaa tctcattacg caaacggaaa tcgccctcag ccagtctcgc
    13621 agccagaagg agctggaaga tgtgctctac tctaatctcg aagagctgac gcgaatggcg
    13681 aaaatggtca gcgatatgct gtttctcgct caggccgata acaaccagct aatccccgaa
    13741 aagaaaatgc tcaacctggc ggatgaagtc ggcaaagtgt tcgatttttt cgaggcgtta
    13801 gcggaagatc gcggcgtgga gttgcgattt gttggcgaca aatgtcaggt tgcgggcgat
    13861 ccgctgatgc tgcgtcgggc gttaagcaac ctgctttcta atgccctgcg ttatacgcca
    13921 accagagaga caattgtagt gcgctgtcag acggtcgatc accaggtgca agttaccgtc
    13981 gaaaaccccg gtacgcccat tgcgcccgag cacttaccgc gattgtttga ccgtttctac
    14041 cgcgttgacc cttcccgcca gcgaaaaggc gaaggtagcg gcattgggct ggcgatagtg
    14101 aaatcgattg ttgtcgcgca taaaggcacg gttgcagtaa cgtcagatgc gcgggggaca
    14161 aggtttgtga ttatgctgcc ggagagagag tgattattaa gttcaaattc agggcgtggt
    14221 gcatcacctc ctggtattgt tgatcaacaa attagagaaa aacttattaa acatatgaaa
    14281 tatcgaaggc ttttattttt caaagtttca cgagatgttt gtatatgaaa cttagttaaa
    14341 tctgtgtttc ttccaattgt agatcacaat gccctttcta aattttcatc gttataaaca
    14401 agccatctat catataaagc aatgaataat ttaatcgcga ttaatcatca ttaattgtta
    14461 tattattcac tcaatctatt tgtcaagtga atttgataaa ttcctaattt aaaatatcca
    14521 ctattgagcc agtatttaac aacgttaaga tgtgcaatgt gcagatcatg ttatcagggt
    14581 ggagcggtag catatatttc ttatgcaatc attcatttta ataatgttaa ttggattatt
    14641 tattgctgtg aataaaaata tattatgaat attggtttaa ctttgcgttt ttgttactcg
    14701 ttgtgatatg cgtacctgtc ctgaataacg ccaagaaaca agaggttgag cttgtgatta
    14761 tttttttgac tgaacagaca cccgtttctg taaagtaata tttaaacaaa attctcacat
    14821 aaccatagca ttttctgttc cttccctaat aacgtctgaa gtcgtcccgc ttatcacatt
    14881 atccgcagtt gtacggtacg gatcattatt cattttactg gaggttgcta tgtcaaccgg
    14941 attacgtttc acactggaag tggacggcct gccgccggac gcttttgcgg tggtttcctt
    15001 tcatctgaac cagtcactct cttcgctctt ttccctcgat ctctccctgg tcagccagca
    15061 gtttctctcc cttgaatttg cgcaggtgct ggacaaaatg gcctacctga cgatatggca
    15121 gggcgatgaa gtacagcgcc gggtgaaagg cgtggtgacc tggtttgaac tcggggagaa
    15181 cgacaaaaac cagatgctgt acagcatgaa ggtgcacccg ccgctgtggc gtgccggtct
    15241 gcgccagaac ttccgtatct tccagaacga ggacatcaaa agcatcctcg gcacgatgtt
    15301 gcaggaaaac ggggtgaccg aatggagtcc gctgttcagc gagccgcatc cttcccgtga
    15361 gttttgtgtc cagtacggtg agactgatta cgatttcctg tgccggatgg cggcggagga
    15421 aggcatcttc ttttatgagg agcatgctta caaaagtacc gaccagagcc tggtgctgtg
    15481 cgacacagtc cgccatctgc ccgaatcttt tgaaatccca tggaacccga acacccgtac
    15541 cgaggtgagc accctctgca tcagccagtt ccgctacagc gcacaaatcc gcccttcttc
    15601 cgtggtgacc aaagactaca cctttaaacg ccccggctgg gccggacgtt ttgaacagga
    15661 aggccagcac caggactacc agcgcacgca gtatgaagtg tatgactacc ccggacgttt
    15721 caagggtgct cacgggcaga actttgcccg ctggcagatg gacggctggc gaaacaatgc
    15781 agaaaccgcg cggggaatga gtcgctcgcc ggagatatgg ccgggacgac gaattgtgct
    15841 gacggggcat ccgcaggcga acctgaaccg ggaatggcag gtggtggcaa gtgaactgca
    15901 cggcgaacag ccacaggcgg tgccaggacg gcagggagcg gggacggcgc tggagaacca
    15961 ttttgcggtg atcccggcag acagaacatg gcgaccacag ccgttgctga aaccgctggt
    16021 cgacggcccg cagagcgctg tcgtgacagg accggcaggc gaggaaatct tctgcgacga
    16081 acatggtcgc gtgcgggtga agttcaactg ggaccgttat aacccggcag accaagacag
    16141 ttcgtgctgg atccgtgtgg cacaggcgtg ggcaggcacc ggttttggcc acctggcgat
    16201 accgcgtgtg ggtcaggaag tgattgtaga cttcctcaac ggcgatccgg accagccgat
    16261 cattatgggg cgcacctacc accaggaaaa ccgccccccc ggcagcctgc cgggaacaaa
    16321 gacgcagatg accatccgct ccaaaacgta catgggcagc ggatttaatg agctgaagtt
    16381 tgatgatgcg acagggagag aacaggtcta catccacgcg cagaagaaca tggataccga
    16441 agtgctcaac gaccgtacca ccaccgtaaa acacgatcac cgcgaaaccg taaaaaatga
    16501 ccagacggtc acgatccagg aaggtaaccg ccttcttacg gtggaaaaag gccacaagat
    16561 caccggagta ctgaaagggt ctttatctga ggatgtcttt caggacagag gcacgattgc
    16621 cggttcggtg catgttgacg ctgtaaacaa tggtggcgaa ggcgacggta tacaggctta
    16681 tacggcgatt aaggaaattt tgctggccgt ggaggaaagc aaaattgcgc tgacgccgga
    16741 tagcattcag ctacaggtcg gggaatcgac ggtaatcagg ctgtcgaagg atggcatcac
    16801 catcgtgggc ggttctgttt tcatcaactg attcactcag attatcaggg agtattttat
    16861 gcagttcact tttaatgaag gccacattca actgccgtct caatggcagg atcagtcaat
    16921 gcaggtcctg gtgtccacag ataacagtgg catcaatctg gtcattaccc gcgaagccgt
    16981 tccacaggga accctgacac cagagctgta ccaggaaacg ctggcgctgt accagggcaa
    17041 acttgatggg tacaccgaac atgcctgccg ggaaatcact ctggcagagg ccccggcctg
    17101 gctgctggat tattcctgga acggtccgga agatgagggg aatcaggggc ggattagcca
    17161 gatagccgta ttccagcggc gtggagatac gctgctgacc ttcactttca gcacctcctt
    17221 atccctgaag aacagtcaaa agacgatgct gctggaggtg ataaagagct tcaccccgtt
    17281 gccaccggag aatgacatcc aaaaggacca gccgcgctga cataaacgag ggataacaca
    17341 tgagtgaagg accaggcggg ccacagggag cgaccgcagg cggtacgctg gcaatgcgaa
    17401 tgctgtcaca gcaggcgatg gtcgccagcc agatgaaacg ggcagccaac gacaaagcca
    17461 ttgcacagat gttggcatca aaaaagtctg gcccccccgc cgccaggctg ggcgatgaaa
    17521 ttcagcacaa gagttttttg ggggcgctgg caggggccgt actgggggcg atagtgacca
    17581 tcgcagaagg ttgcctgatt atggccgcct gtgccaccgg cccttatgcg ctggttctgg
    17641 tacctgcgct gatgtatgcc agctataagg cgagtgatta tgtggaggag aaacagaacc
    17701 agcttgaatc atggataaac agcttttgtg acacggacgg cgccatcaat accggttctg
    17761 aaaacgtaaa aattaacggc gagctggccg cacgtgcagc ggtcaccctt ccccctcctc
    17821 ccccacctgg agcaatacct gaagtcccac agggggaacc ctcatggggt gatattgcca
    17881 ctgacctgct tgaatcggca gcggaaaaag cagtaccact ggcgaaggcc tgggggaacg
    17941 ctgttatcac cctgacggac agcaatgccg gttttatgga tcgcgtcagc gccggcgcat
    18001 cgcttctgtt tcccgccggt ccggtattaa tggagtttgc caccatggtg ggcgggcgtg
    18061 gcgaaatcaa aaaagatgtg gatttcccgg aagccggtga ggacacggcg ctctgcgaca
    18121 aggagaacaa accaccgagg atagcccagg gcagcagcaa cgtctttatc aacaatcagc
    18181 ctgccgcgcg caagggcgac aaactggagt gcagtgcggc gatcgtggga ggttcgccgg
    18241 acgtctttat tggcggtgag caggtcacat atctggatat ccagccggag ttcccgccgt
    18301 ggcagagaat gatcctgggg ggaataacga tagccagcta tcttctgccg ccagcgggac
    18361 tactgggaaa actgaagaat ctggcgagac tcggcaaact gggaaactgc tggggaaaag
    18421 cgggaagctg ctgggcgcaa agctcggcgc gttgctgggg aaaacaggta agtcgttaaa
    18481 aagtattgcc aataaagtca tcagatgggt aacagatcct gtcgatccgg taaccggcgc
    18541 atactgcgac gaacgtaccg acttcaccct gggccagacc ctccccctct ccttcacccg
    18601 tttccacagt tctgtactgc cgctgcatgg cctgaccggc gtgggctgga gcgactcctg
    18661 gagcgaatac gcctgggtgc gtgaacaggg aaaccgggtg gatatcatca gccagggagc
    18721 cacgctgaga tttgccttcg acggtgacag tgatacggcg gttaacccgt atcacgccca
    18781 gtacattctg cgccgccgcg atgattatct ggagctgttc gacagggatg cactgagcag
    18841 ccgcttcttt tatgacgcct ttccgggaat gcgtctgcgc cacccggtga ctgacgatac
    18901 cagcgatgac cgcctggcac acagccccaa tgaccggatg tacatgctgg gcgggatgag
    18961 cgacaccgcc agcaaccgca tcacgtttga gcgcgacagc cagtaccgga tcacgggtgt
    19021 cagtcacacc gacgggatcc ggcttaaact gacgtaccac gccagcggct acctgaaagc
    19081 cattcaccgc acggataacg acatacagac gctggcgacc tacgaacagg atgcgcgggg
    19141 gcggctgaca gaagcggatg cgcggctgga ctaccacctg ttttatgagt acgacgctgc
    19201 ggaccggatc atccgctggt ccgataacga ccagacgtgg agccgtttca cctacgatga
    19261 acagggccgg tgcgtgaatg tcaccggggc ggagggctat tacaacgcca cgctggacta
    19321 tggtgacggc tgcaccaccg tgacggacgg caagggcatt caccgttatt actatgatcc
    19381 tgacggcaat attctgcggg aagaagcgcc ggacggcagc accaccatgt atgaatggga
    19441 tgaattccat cacctgctgg cccgccactc ccctgccggg cgggtggaga aatttgaata
    19501 caacgccgca cacggtcagt taagccgtta cacggcggca gacggcgcgg agtggcagta
    19561 ccgctatgat gagcgcggcc tgctcagcaa catcaccgac cctgccgggc agacgtggac
    19621 acagcagtgt gatgaacgcg gcctgccggt aagtctggtg tcgccacagg gcgaagagac
    19681 ccggctggca tacaccgctc aggggctgct gtcgggaata ttccgccagg atgaacggcg
    19741 tctgggcata gagtacgacc accacaaccg gccggaaaca ctcaccgacg tgatgggccg
    19801 tgaacaccac accgaataca gcggtcacga cctgccagtg aagatgcgcg gccccggcgg
    19861 tcagtcagtg cggttacagt ggcagcagca ccataaactg agcggcattg aacgtgctgg
    19921 aaccggcgcg gaaggatttc gctatgaccg ccacggcaac ctgctggcct ggacggacgg
    19981 caacggtgtt gtctggacga tggaatacgg tccgttcgat tttccggtgg cgcgaacgga
    20041 cggtgaaggc caccgctggc agtaccgcta cgataaagac acgctgcaac tgacagaagt
    20101 catcaacccg cagggtgagt catatcttta tgttctggat aactgcggcc gggtgacgga
    20161 agagcgtgac tggggcggcg tggtctggcg ttaccgctat gacgccgatg gcctctgtac
    20221 cgccaaggtt aacggcctgg aggaaaccat cctctacagc cgggacgccg caggccgcct
    20281 ggcagaaatc atctcgccgg aaggcaaaac gcagtatgcc tatgacaaat ccggcaggct
    20341 gacgggtatc ttcagcccgg acggcacatc acagcgcacc ggctatgacg aacgcgggcg
    20401 ggtgaatgtc accactcagg gccgacgggc cattgaatac aactaccccg atgaacacac
    20461 cgtcatccgc tgtatcctgc caccggaaga tgaacgcgac agacaccccg gtgaatccct
    20521 gctgaaaacc acgtaccgct acaacgccgc cggagaactg acagaggtca ttctgccggg
    20581 ggatgagacg ctgacgttca gccgtgatga ggcgggacgt gaagtgctcc ggcacagtaa
    20641 ccggggtttt gcctgtgaac agggctggaa tgcagccggt cagcctgtca gtcagcgcgc
    20701 cggatttttt ccggcagaag ccacatggga cgggctggtt ccctcactgg tacgggagta
    20761 ccgttacgac agcgcgggta acgtgtcagg cgtcaccagc cgggaagatt acggacggga
    20821 aacacggcgg gagtatcggc tggaccggaa cggccaggtc acggcggtga cagcctcagg
    20881 caccgggctg ggctatggcg aaggcgatga gtcctatggc tatgacagct gcggctacct
    20941 gaaggcgcag tctgcgggca ggcaccggat aagtgaagag actgaccagt atgccggagg
    21001 ccaccggctg aaacaggccg gaaacacgca gtatgactat gacgccgcag gccggatggt
    21061 cagccgcacc agacaccgtg acggctaccg accggaaaca gagcggttcc ggtgggacag
    21121 ccgggaccag ctgaccgggt attgcagcgc acagggtgag ctgtgggaat accgccacga
    21181 cgccagcggc agacgaacag agaaacgctg cgaccggaag aaaatccgct tcacgtatct
    21241 gtgggacggc gacagtattg cggaaatccg ggaataccgc gatgataaac tgtacagcgt
    21301 aaggcacctg gtgtttaacg gctttgagct gataagccag cagttcagcc gggtacggca
    21361 ggcgcatccg tcggtggccc cgcagtgggt gacgcggacg aatcatgcgg tgagcgacct
    21421 gacgggccgc ccgctgatgc tctttaacag tgaaggaaag acggtctggc gaccggggca
    21481 gaccagcctg tgggggctgg cactcagcct gcccgcagac accggctacc cggacccgcg
    21541 cggggaactg gacccggaag ccgaccccgg cctgctgtat gcaggacagt ggcaggatgc
    21601 ggaatccggg ctgtgctata atcggttccg gtattacgag ccggaaaccg gaatgtacct
    21661 ggtgagtgat ccgctggggt tgcagggcgg ggagcagact taccggtatg tgccgaatcc
    21721 ttgtgggtgg gttgatccgc tgggattggc tgcaagttct aaaatcagca gtttgatgga
    21781 ctatattggc gatggtcgtc gtgttagtgg gcatacgggt ttcctggatg gggttcgttt
    21841 atcacgtagt caaataa
//
LOCUS       DAJVQG010000064        21643 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJVQG010000064 DAJVQG010000000
VERSION     DAJVQG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.064"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..986)
                     /gene="cmtA"
                     /locus_tag="OQM83_002135"
     CDS             complement(<1..986)
                     /gene="cmtA"
                     /locus_tag="OQM83_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCS1520398.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
     gene            complement(1014..1457)
                     /gene="cmtB"
                     /locus_tag="OQM83_002136"
     CDS             complement(1014..1457)
                     /gene="cmtB"
                     /locus_tag="OQM83_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HCS1520399.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            complement(1771..3762)
                     /gene="tkt"
                     /locus_tag="OQM83_002137"
     CDS             complement(1771..3762)
                     /gene="tkt"
                     /locus_tag="OQM83_002137"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCS1520400.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            4040..4798
                     /gene="loiP"
                     /locus_tag="OQM83_002138"
                     /gene_synonym="yggG"
     CDS             4040..4798
                     /gene="loiP"
                     /locus_tag="OQM83_002138"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCS1520401.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4941..5861)
                     /gene="speB"
                     /locus_tag="OQM83_002139"
     CDS             complement(4941..5861)
                     /gene="speB"
                     /locus_tag="OQM83_002139"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCS1520402.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5999..7975)
                     /gene="speA"
                     /locus_tag="OQM83_002140"
     CDS             complement(5999..7975)
                     /gene="speA"
                     /locus_tag="OQM83_002140"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCS1520403.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7984..8115)
                     /gene="yqgB"
                     /locus_tag="OQM83_002141"
     CDS             complement(7984..8115)
                     /gene="yqgB"
                     /locus_tag="OQM83_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCS1520404.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            8251..8466
                     /locus_tag="OQM83_002142"
     CDS             8251..8466
                     /locus_tag="OQM83_002142"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1520405.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            8770..9924
                     /gene="metK"
                     /locus_tag="OQM83_002143"
     CDS             8770..9924
                     /gene="metK"
                     /locus_tag="OQM83_002143"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCS1520406.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            10360..11754
                     /gene="galP"
                     /locus_tag="OQM83_002144"
     CDS             10360..11754
                     /gene="galP"
                     /locus_tag="OQM83_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCS1520407.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            11831..12328
                     /locus_tag="OQM83_002145"
     CDS             11831..12328
                     /locus_tag="OQM83_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCS1520408.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            12423..13130
                     /gene="endA"
                     /locus_tag="OQM83_002146"
     CDS             12423..13130
                     /gene="endA"
                     /locus_tag="OQM83_002146"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCS1520409.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMYNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            13210..13941
                     /gene="rsmE"
                     /locus_tag="OQM83_002147"
     CDS             13210..13941
                     /gene="rsmE"
                     /locus_tag="OQM83_002147"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCS1520410.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            13954..14904
                     /gene="gshB"
                     /locus_tag="OQM83_002148"
     CDS             13954..14904
                     /gene="gshB"
                     /locus_tag="OQM83_002148"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCS1520411.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTENGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            15013..15576
                     /locus_tag="OQM83_002149"
     CDS             15013..15576
                     /locus_tag="OQM83_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCS1520412.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            15576..15992
                     /gene="ruvX"
                     /locus_tag="OQM83_002150"
     CDS             15576..15992
                     /gene="ruvX"
                     /locus_tag="OQM83_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCS1520413.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(16167..17147)
                     /locus_tag="OQM83_002151"
     CDS             complement(16167..17147)
                     /locus_tag="OQM83_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCS1520414.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            17165..17869
                     /gene="yggS"
                     /locus_tag="OQM83_002152"
     CDS             17165..17869
                     /gene="yggS"
                     /locus_tag="OQM83_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCS1520415.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17887..18453
                     /gene="yggT"
                     /locus_tag="OQM83_002153"
     CDS             17887..18453
                     /gene="yggT"
                     /locus_tag="OQM83_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCS1520416.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            18450..18740
                     /locus_tag="OQM83_002154"
     CDS             18450..18740
                     /locus_tag="OQM83_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCS1520417.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIVALIN"
     gene            18748..19341
                     /locus_tag="OQM83_002155"
     CDS             18748..19341
                     /locus_tag="OQM83_002155"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCS1520418.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            19334..20470
                     /gene="hemW"
                     /locus_tag="OQM83_002156"
     CDS             19334..20470
                     /gene="hemW"
                     /locus_tag="OQM83_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCS1520419.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            20781..>21643
                     /locus_tag="OQM83_002157"
     CDS             20781..>21643
                     /locus_tag="OQM83_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCS1520420.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSEGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQKQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMG"
BASE COUNT         5355 a         5635 c         5513 g         5140 t
ORIGIN      
        1 gcaaaggaca ccagggtagc taccgtaaca ccggcaattg tcgccaggaa cgagcctttc
       61 ggcgtcagtg ccaggtaagc aaagatagac cccggacttg ggccagccac cagaccaccg
      121 tccagtaagt taaacaccca ggtgccagac ataccgcccg caatcatggc aataatggtc
      181 agcggcttca tcagcacata cgggaaatac agttcgtgga tcccaccgag gaagtgaata
      241 atcatcgccc cgggcgcaga acgtttactc atccctttac caaacaaggt aaacgccagc
      301 agcagcccta agccaggccc aggattggac gccaccataa agaagatgga tttaccgtta
      361 accgaagcct gttgcattcc cagcggataa tagacgccct gatcgatcgc attattgagg
      421 aaaagcactt tcgccggttc attgatgact gacagcagag gcagataacc cgcatgtacc
      481 agcgcctcaa tacactcttt gacgagagta ttggcaatta acaccgccgg gccgataact
      541 tcaaaaccca gcagacaaag gagcatcccc gcgataccta atgagaagtt attgataacc
      601 atctcaaaac cggcaggtat gcgtttttcc agtgccttat caacatattt tatgaccagc
      661 ccaccgagcg gccccataat cattgagcca aggaacatcg ggatctctgc gccgacgatc
      721 acaccaatag tacctattcc gcccatgacc gcgccgcgtt taccgccgac cagatgacca
      781 ccggtagaac caatcatcac gggcaataaa taggtaatca tcgggccgac aattttggcg
      841 aaatgttcgt taggcagcca accggtggga ataaataacg cagtaataaa accccaggca
      901 ataaaagcac caatattggg gatgaccatt gccgtcaaaa agcccccaaa agcctggacc
      961 tttgcacgag cagacttgtt ttccataata ttatcctgta gaggagagag taattatccg
     1021 cggctgatta tgtccgctaa ttgtttttct gatgctgctg ttaagagttg ttcgagtatt
     1081 tcttcttcgc acagtaattc acttaacgcc tgaatagcgc caatgtgcga atcggcatcg
     1141 gcagcagaaa ggccgatgag taattttatt ggttcgtcat tccccggaaa ataaacacct
     1201 tgttcaagta atgtcaaaga catcccggtt ttaagcgccc cacattccgg tcgcgcatga
     1261 ggcattgcca cgcctggtgc gagaatataa taagggccat tgttaatggt ggaatcttta
     1321 attgcctgaa tgtaattctc gctgatatag tttttatcca gcaatgatac catcgagaaa
     1381 tcgatagctt cctgccaatc ttttgctgaa tgtataactg agattgatga ttctggaaaa
     1441 tagtcactaa gccgcataaa ttatccttat tttattttta gggtaccgtc cggcggtaaa
     1501 aataaaatac tcgtgcggtt taatgaaagc aattgagcag ggaaaccggt gttttgaaaa
     1561 cagtgaatta ttaatttctt atataacatt gagttataga tataacaaca aatgtcaata
     1621 cgcatatcgt gatgcatatt acataattga tatttattgc atataaatat ttgtgatcta
     1681 caacacgctt tatctattgc ttgtccgcaa acggacatat caaggtaata aaaaaggtcg
     1741 ccgaagcgac ctttttaccc gaaatgctaa ttacagcagt tcttttgctt tcgcaacgac
     1801 gttgtcgacg gtgaagccga actcttcgaa cagcaactct gccggagctg actcaccgaa
     1861 ggtggtcata ccgatgatag cgccattcag gccaacatac ttgtaccagt agtcagcaat
     1921 acccgcttct accgcaacgc gtgcagtaac cgctttcggc agtacggatt cacggtaagc
     1981 cgcatcctgc ttgtcgaatg catcggtaga cggcatggaa accacgcgcg ccttcacgcc
     2041 ttcggcagtc agtttttcgt aggcggcaac agccagttca acttccgaac cggtcgcgat
     2101 gaagatcagt tccggctgac cagcacagtc tttcagcaca taaccaccgc gcgcgatgtt
     2161 tgccagttgc tcttcagtac gttcctgctg cgccagattc tgacgggaga ggataagcgc
     2221 ggtcggaccg tcctgacgct caacaccgta tttccacgcg accgcggatt caacctggtc
     2281 acacggacgc catgtagaca tgttcggggt cacgcgcaga gaagcgacct gctcaaccgg
     2341 ctggtgagtc gggccgtctt cgcccagacc gatggagtcg tgggtgtaaa ccatcacctg
     2401 acgctgtttc atcagcgcag ccatacgtac agcgttacgt gcgtattcga caaacatcag
     2461 gaaggtggag gtgtacggca ggaagccacc gtgcagggag ataccgttag caatcgcggt
     2521 cataccgaac tcgcgaacac cgtagtggat gtagttaccc gcagcatctt cgttgattgc
     2581 tttagaacca gaccacaggg tcaggttaga cggtgccagg tcagcggagc cgccgaggaa
     2641 ttccggcaac agcgggccga acgcttcaat agcattctga gacgctttac ggctggcgat
     2701 tttcgccgga ttagcctgca gtttagcgat aaactctttc gctttggcgt cgaagtcaga
     2761 cggcatttcg cctttcatac ggcgggtaaa ttcagccgct tcctgcggat aagctttcgc
     2821 gtaagccgca aacttctcat tccatgcaga ttctttcgcc tggcctgctt ctttcgcatc
     2881 ccactgcgca tagatttcag acgggatttc gaacggtgcg tatttccagc ccagctgttc
     2941 gcgggtcaga gcgatttctg catcacccag cggcgcaccg tgggagtcgt gagtaccggc
     3001 tttgttcggg gaaccgaaac cgatgatggt tttgcacatc agcagggacg gtttgtcagt
     3061 cactgcgcgc gcttcttcta ctgcgcgttt gatggatgct gcgtcatgac cgtcgatgtc
     3121 gcgaataacg tgccagccgt aagcttcgaa acgcattgcg gtgtcgtcgg tgaaccagcc
     3181 ttcaacgtga ccatcaatag agataccgtt gtcatcgtag aacgcaatca gtttacccag
     3241 cttcagcgta cccgccagag agcaaacttc gtgggagatg ccttccatca tgcagccgtc
     3301 gcccatgaag gcgtaggtgt agtggtcgac aatgtcgtgg cccggacggt taaactgcgc
     3361 cgccagcgtt ttttctgcaa tcgccatacc gactgcgttg gcaataccct gacccagcgg
     3421 accggtggtg gtttccacac cagcggtata acctacttcc gggtggcccg gagttttaga
     3481 gtgcagctga cggaagtttt tcagttcttc catcggcaga tcgtaaccgg tgaggtgcag
     3541 caggctgtag atcagcatgg agccgtggcc gttggacagc acgaagcggt cacggtcagc
     3601 ccaggacgga ttctgcgggt tgtgtttcag gaaatcacgc cacaggactt cggcaatgtc
     3661 agccataccc ataggggcac ccgggtgacc ggatttggct ttctgtactg cgtccatgct
     3721 cagcgcacga atagcattgg caagctcttt acgtgaggac attttgactc cagatcggat
     3781 gatgaagggc acgcccttaa cgacttgacg acagcgcgtt ttgggctacg ccggaaaatt
     3841 tgccaacaat ttaccgcaag ccgcgcgtca tgtacatgga acatcctttt gccgcttcag
     3901 aaatctctgg atcatgctcg catgttgcgc aatctactcg cccgtccgct gcgcttttcc
     3961 ttatactgag actgagcgtc gattcacctg caaacggcgc atttttagaa taatcctgac
     4021 cttgtgcgga agagaaaaca tgaaaattcg cgccttattg gtagcaatga gcgtggcaac
     4081 ggtactgact ggttgccaga atatggactc caacggactg ctctcatcag gagcggaagc
     4141 ttttcaggct tacagtttga gtgatgcgca ggtgaaaacc ctgagcgatc aggcatgtca
     4201 ggagatggac agcaaggcga cgattgcgcc agccaatagc gaatacgcta aacgtctgac
     4261 aactattgcc aatgcgctag gcaacaatat caacggtcag ccggtgaatt acaaagtgta
     4321 tatggcgaag gatgtgaacg cctttgcaat ggctaacggc tgtatccgcg tctatagcgg
     4381 gctgatggat atgatgacag ataacgaagt cgaagcggtg atcggtcacg agatggggca
     4441 cgtggcgtta ggccatgtga agaaagggat gcaggtggcg cttggtacaa atgccgtgcg
     4501 agtagctgcg gcctctgcgg gcgggattgt cggcagttta tctcaatcac aacttggtga
     4561 tctgggcgag aaattagtca attcgcaatt ctcccagcgc caggaagcag aagccgatga
     4621 ttattcttac gatcttctgc gccaacgcgg catcagcccg gcaggtcttg ccaccagctt
     4681 tgaaaaactg gcgaaactgg aagaaggccg ccaaagctca atgtttgacg accatccggc
     4741 atccgccgaa cgcgcccagc atattcgcga tcgcatgagc gcggatggga ttaagtaaag
     4801 cctggtggtg tcgaacgtag gtcagataag gcgttcacgc cgcatctgac atcaatggca
     4861 catttgttac cctgtgcgca ttgccggatg cgatgctgac tgcatcttat ccggcctacg
     4921 ggtggcatcg catctggtgc ttactcgccc tttttcgccg cttggatata cagcatctcc
     4981 agcgccagcg tcgccgcagc cagagcggtg atttccgact gatcgtatgc cggagccact
     5041 tccactacgt ccatcccaac aatgttgaga tctttcaggc cgcgtaccag tttaatagcg
     5101 cgatcggagg tcaggccgcc aatcactggc gtaccggtgc ctggtgcaaa agcaggatcc
     5161 aggcagtcga tatcaaaagt caggtaaacc ggcatatcac ccacaatctg tttcacttgg
     5221 gcgataacgt catccacgct gcgatcgttc acctggcagg cgtccagcac ggtaaagccg
     5281 ttgtctttat caaactcggt acgaatacca atctgcacgg aatgattcgg gtcgatcaga
     5341 ccttctttcg gcgcggtata gaacatagtg ccgtggtcaa attcacaacc gttcgcatag
     5401 gtatcggtgt gggcgtcaaa gtgtaccagc gccattttgc cgaaatgctt cgcatgagca
     5461 cgcagcagcg gcagcgtaac aaagtggtca ccaccgaagg tgagcatacg cttaccggca
     5521 gccagcagct tctcggcgtg cgcctgcagc ttttcgctca tctcacgggc atcgccaaag
     5581 gcatatacca gatcgccgca gtccacgacg ttcagacgct cacgcatgtc gaaattccac
     5641 gggaagcggt tgtgttccca ggccagattc gtcgaaacct gacggatcgc tgccggaccg
     5701 tggcgaccac ccgcacgacc agaagtggcc atatcgaacg gcacgccagt aatcacccag
     5761 tctgcatcgc tgtcatacgg ctggaagttc atcggcaggc gtaaaaaacc aaaggcattg
     5821 gaaaccagtg agttatcgta ttgatgacct aaggtgctca tggatggact cctattacaa
     5881 agtcgatata aaaaaaccct tccgcgtcgt taggcccgac gaggaagggt tggatttgtc
     5941 acaataaatt gtggcggatt atcaccgcta aattaagcgg attcaagtaa cacaggactt
     6001 actcatcttc aagataagta taaccgtaca aacctgcctc gaactcttca aggaactgtt
     6061 gttgcagttc agcatcaaga tcggttttct tcacttgatc gcggaactgg gttaacagcg
     6121 ttttcggatc gagctgtaca tattgcagca tgtccgccac ggtatcgcct tcgtcagaca
     6181 gttctacttc tacgctaccg tcagggaaca cgaacacgtc aaccgcttcg gtatcaccga
     6241 acaggttgtg catattgcca aggatctcct gatatgcgcc gaccataaag aaaccgagca
     6301 tcggcggatt ctctggatcg tactccggca ttggcattgt cgtggcaata ccatcaccat
     6361 caatatagtg gtcgatagca ccgtcagagt cacaggtaat atccagcagc acagcgcgac
     6421 gttccggcac ttgatccagc ccttccagcg gcagaaccgg gaacaactgg tcgatccccc
     6481 atgcgtccgg cattgactgg aacagcgaga aattgacgta cattttgtcc gccatacgtt
     6541 cctgcagttc gtcgattatc ggacgatgcg cacggttttg cggatccagt tgcttctgca
     6601 cttcatggca catgctcaga tagagttgtt ctgcccatgc acgttcttgc aggctaaagg
     6661 tgccggaaga gtagccgata tgaatgtcgt gcagatccat ctggctgtcg tgtaaccatt
     6721 cacgcaaaga acggcgagtt cccggttcgt gcatttcctg ccaggtttcc cacatgcttt
     6781 gcagcgcgcg cggcgcatct tctgcgggcg cggtcggcac cgtatattcg ttacgttcca
     6841 cgccgatgat attcgacacc agcacggtat ggtgcgcagt caccgcacgg cccgattcgg
     6901 tgattaccgt cggatgcggc agaccatttt cttcacacgc atcgccaatc gcccagatga
     6961 tgttgttggc gtattcattg aggccatagt ttacggagca gtcagactgc gaacgggtgc
     7021 cttcatagtc cacgcccaga ccgccgccga cgtcgaagca ctgaatattg acgcccagct
     7081 tgtgcagttc cacatagaaa cgcgcggatt cacgaacgcc cgtggcgata tcgcgaatat
     7141 tcgccatctg cgaaccgagg tggaagtgca gtagttgcag gctgtcgaga cgcccggctt
     7201 cacgcagggt ttcaaccagt tgcagtacct gagtcgcagc caggccgaac ttcgattttt
     7261 ccccgccgga ggactgccat ttacccgagc cctgcgaagc cagacgtgca cgcacgccca
     7321 gacgaggaac gacattcaga cgttctgctt catccagcac aatggcgatt tctgacatct
     7381 tctcaatgac cagatagacc ttgtgcccca tcttctcgcc aattaatgcc aggcggatat
     7441 attcgcggtc tttataaccg ttgcagacga tgacgctacg ggtcatgcca gcatgtgcca
     7501 gtactgccat caactcggct ttggaaccgg cttccagacc cagcggttcg cccgaatgaa
     7561 tcagggactc aatcacgcgg cggtgctggt taactttgat cggataaaca aggaagtaat
     7621 cgccgttata gccgtaggat tccctcgcac gtttgaacgc ggcgttaatg gaacgcaaac
     7681 ggtgctgcag gatctgtggg aaacagaaca gtgcaggcag acgctgaccc tgtgcttcac
     7741 gagttttcac taactgcgcg agatcgacgc gagcttccgg gacgtccggg tccgggcaca
     7801 cgctgatgtg gcccagctcg ttaacgtcat agtagttatt gccccaccag gcaatattgt
     7861 aagtacgcag catcttgctg gcttcctggg agctcattgc aacctcctgc atggagcgta
     7921 gtacaccgtg ttcgcccgct gacgaaggca aacccataga catgtcgtca gacatagcga
     7981 acctcaaatt attttattaa gtgtaaaaca gttaacgact atcgcagcct ggaactgcga
     8041 taacaaccca taagcacatg gattttccag cagtgaatgc tgacgctcca actgcgcaac
     8101 cggtttcttt ttcataacat tattaagcac ataaccgaac gtaagtgtga cagttcggcg
     8161 aaaccacgag aaaactcttg tttttacaag agcgcccttg ttcagtcctc agtaactgta
     8221 accagctctt gaatcctgag aagcgccgag atgggtataa catcggcagg tatgcaaagc
     8281 agagatgcag agtgcgggga acgaatcttc accagaacgg tgagacaggt taagcagcag
     8341 acaacggttc attatttcgt atcacctcca cggccgcctg ttaagacgaa cccacaagcc
     8401 aaaactctga tttcaacccg gctggaagtg gcaacacgaa agaaacgtcg tgtgcttttt
     8461 atttaagccg cgcgccgcgt tttatacccc acaatggcag aaaattgcaa aagataaata
     8521 cgcagaatgc cggcattgtc aggaaaaatt tccagccacg tttttaacag aatgagacac
     8581 gattcaaaaa aaagtggaaa tcgggtgaag aattgaccta aaatagccat ccagatgtta
     8641 atccatccat accgattaac actcagactg ccagtgtttt taacctgcag agtcgtggta
     8701 ggatccgcta ccacagaaaa tccacacaac agtttgagct aaccaaattc tctttaggtg
     8761 atattaaata tggcaaaaca cctttttacg tccgagtccg tctctgaagg gcatcctgac
     8821 aaaattgctg accaaatctc tgatgccgtt ttagacgcga tcctcgaaca ggatccgaaa
     8881 gcacgcgttg cttgcgaaac ctacgtaaaa accggcatgg ttttagttgg cggcgaaatc
     8941 accaccagcg cctgggtaga catcgaagag atcacccgta acaccgttcg cgaaattggc
     9001 tatgtgcatt ccgacatggg ctttgacgct aactcctgtg cggttctgag cgctatcggc
     9061 aaacagtctc ctgacatcaa ccagggcgtt gaccgtgccg atccgctgga acagggcgcg
     9121 ggtgaccagg gtctgatgtt tggctacgca actaatgaaa ccgacgtgct gatgccagca
     9181 cctatcacct atgcacaccg tctggtacag cgtcaggctg aagtgcgtaa aaacggcact
     9241 ctgccgtggc tgcgcccgga cgcgaaaagc caggtgactt tccagtatga cgacggcaaa
     9301 atcgttggta tcgatgctgt cgtgctttcc actcagcact ctgaagagat cgaccagaaa
     9361 tcgctgcaag aagcggtaat ggaagagatc atcaagccga ttctgcccgc tgaatggctg
     9421 acttctgcca ccaaattctt catcaacccg accggtcgtt ttgttatcgg tggcccgatg
     9481 ggtgactgcg gtctgactgg tcgtaaaatt atcgttgata cctacggcgg catggcacgt
     9541 cacggtggcg gagcattctc tggtaaagat ccatcaaaag tggaccgttc cgcagcctac
     9601 gcagcacgtt atgtcgcgaa aaacatcgtt gctgctggcc tggccgatcg ttgtgaaatt
     9661 caggtttcct acgcaatcgg cgtggctgaa ccgacctcca ttatggtaga aactttcggt
     9721 actgagaaag tgccttctga acaactgact ctgctggtac gtgagttctt cgacctgcgc
     9781 ccatacggtc tgattcagat gctggatctg ctgcacccga tctacaaaga aaccgcagca
     9841 tacggtcact ttggtcgtga acatttcccg tgggaaaaaa ccgacaaagc gcagctgctg
     9901 cgcgatgctg ccggtctgaa gtaatctttc ttcacctgct ttcaaaggcc agcctcgcgc
     9961 tggccttttt cttttacctc accgcggtgg ataggcattc acgccgcatc cggcaaaaag
    10021 ctgcccgcac aataacatca ttcttcctca acacgtttta tcgcaaatta tcatcactac
    10081 tgaaaccgat tacaccaata acaccagcca aatatttgta atattttcat attattattc
    10141 agttttcaca gttgttacat ttcttttcag taaagtctta attgcagata acagcgttta
    10201 atctatgatg atataactca attattttca tgcacttaaa ccataactaa gataaatgtt
    10261 agtgtaagcg attacactga tgtgatttgc ttcacatctt tttacgtcgt actcacctat
    10321 cttaattcac aataaaaaat aaccatattg gagggcatca tgcctgacgc taaaaaacag
    10381 gggcggtcaa acaaggcaat gacgtttttc gtctgcttcc ttgccgctct ggcgggatta
    10441 ctctttggcc tggatatcgg tgtaattgct ggcgcactgc cgtttattgc agatgaattc
    10501 cagattactt cgcacacgca agaatgggtc gtaagctcca tgatgttcgg tgcggcagtc
    10561 ggtgcggtgg gcagcggctg gctctccttt aaactcgggc gcaaaaagag cctgatgatc
    10621 ggcgcaattt tgtttgttgc cggttcgctg ttctctgcgg ctgcgccaaa cgttgaagta
    10681 ctgattcttt cccgcgttct actggggctg gcggtgggtg tggcctctta taccgcaccg
    10741 ctgtacctct ctgaaattgc gccggaaaaa attcgtggca gtatgatctc gatgtatcag
    10801 ttgatgatca ctatcgggat cctcggtgct tatctttctg ataccgcctt cagctacacc
    10861 ggtgcatggc gctggatgct gggtgtgatt atcatcccgg caattttgct gctgattggt
    10921 gtcttcttcc tgccagacag cccacgttgg tttgccgcca aacgccgttt tgttgatgcc
    10981 gaacgcgtgc tgctacgcct gcgtgacacc agcgcggaag cgaaacgcga actggatgaa
    11041 atccgtgaaa gtttgcaggt taaacagagt ggctgggcgc tgtttaaaga gaacagcaac
    11101 ttccgccgcg cggtgttcct tggcgtactg ttgcaggtaa tgcagcaatt caccgggatg
    11161 aacgtcatca tgtattacgc gccgaaaatc ttcgaactgg cgggttatac caacaccaca
    11221 gagcaaatgt gggggacagt gattgtcggc ctgaccaacg tacttgccac ctttatcgca
    11281 atcggccttg ttgaccgctg gggacgaaaa ccaacgctaa cgctgggctt cctggtgatg
    11341 gctgctggca tgggcgtact cggtacaatg atgcatatcg gtatccactc tccgtcggcg
    11401 cagtatttcg ccatcgccat gctgctgatg tttattgtcg gttttgccat gagtgccggt
    11461 ccgctgattt gggtactgtg ctccgaaatt cagccgctga aaggccgcga ttttggcatc
    11521 acctgctcca ctgccaccaa ctggattgcc aacatgatcg ttggcgcaac gttcctgacc
    11581 atgctcaaca cgctgggtaa cgccaacacc ttctgggtgt atgcggctct gaacgtactg
    11641 tttatcctgc tgacattgtg gctggtaccg gaaaccaaac acgtttcgct ggaacatatt
    11701 gaacgtaacc tgatgaaagg tcgtaaactg cgcgaaatcg gcgctcacga ttaatctcct
    11761 caggcttcct cccgtcgcgg aggaagccgc ctcttgcagt catcttttct tcgctctatc
    11821 ctctgccgct atgaaaacat cccgtctccc tatcgccatc caacaggccg ttatgcgcgg
    11881 cctgcgggaa aaactcgccc aggccaacct gaagctaggg cgtaactacc cggagccaaa
    11941 actctcttac acccagcgcg gaacctccgc cggaacagcc tggctggaaa gctatgaaat
    12001 ccgcctcaat cccgttttgc tgttggaaaa cagtgaagct tttattgaag aagtggtacc
    12061 gcacgaactg gcacatttgc tggtatggaa acatttcggc cgcgtagcgc cacatggcaa
    12121 agagtggaag tggatgatgg aaagcgtgct gggtgttccc gcccgtcgta cgcatcagtt
    12181 cgaactgcaa tccgtgcgtc gcaacacctt cccctaccgc tgcaagtgcc aggagcatca
    12241 gcttaccgta cgccgccata atcgcgtagt tcgtggcgag gccgtctatc gctgtgttca
    12301 ctgcggtgaa cagctggttg cgaaataacc atctgaacta tcaggaactt tcctgatctg
    12361 gctgattgca taccaaaaca actttcgcta cgttgctggc tcgttttaac acggagtaag
    12421 tgatgtaccg ttatttgtct attgctgcgg tggtactgag cgcagcattt tccggcccgg
    12481 cgttggccga aggtatcaat agtttttctc aggcgaaagc cgcggcggta aaagtccacg
    12541 ctgacgcgcc cggtacgttt tattgcggat gtaaaattaa ctggcagggc aaaaaaggcg
    12601 ttgttgatct gcaatcgtgc ggctatcagg tgcgcaaaaa tgaaaaccga gccagccgcg
    12661 tagagtggga acacgtcgtt cccgcctggc agttcggtca ccagcgccag tgctggcagg
    12721 acggtggacg taaaaactgc gctaaagatc cggtctatcg caagatggaa agcgatatgt
    12781 ataacctgca gccgtcagtc ggtgaggtga atggcgatcg cggcaacttt atgtacagcc
    12841 agtggaatgg cggcgaaggc cagtacggtc aatgcgccat gaaggtcgat ttcaaagaaa
    12901 aagctgccga accaccagcg cgtgcacgcg gtgccattgc gcgcacctac ttctatatgc
    12961 gcgaccaata caacctgaca ctctctcgcc agcaaacgca gctgttcaac gcatggaaca
    13021 agatgtatcc ggttaccgac tgggagtgcg agcgcgatga acgcatcgcg aaggtgcaag
    13081 gcaatcataa cccgtatgtg caacgcgctt gccaggcgcg aaagagctaa cctacactag
    13141 cgggattctt tttgttaacc cctaccccac gcgtacaacc gcgtggggag acgacgcgga
    13201 tttttaacta tgcgtatccc ccgcatttat catcctgaac cactgaccag ccattctcac
    13261 atcgcgcttt gcgaagatgc cgccaaccat atcgggcgcg tactgcgcat ggggccgggg
    13321 caggcgttgc aattgtttga cggtagcaac caggtctttg acgccgaaat taccagcgcc
    13381 agcaaaaaaa gcgtggaagt gaaggtgctg gaaggcaaga tcgacgatcg cgaatctccg
    13441 ctgcatattc acctcggtca ggtgatgtcg cgtggtgaaa aaatggaatt tacgatccag
    13501 aaatcgatcg aactcggtgt aagcctcatt acgccacttt tttctgagcg ctgcggcgtt
    13561 aaactggata gtgaacgtct gaacaagaag ctccagcagt ggcagaagat tgcaattgct
    13621 gcctgtgagc agtgtggtcg taaccgggtg ccggaaatcc gtccagcgat ggatctggaa
    13681 gcctggtgtg cagagcagga tgaaggactg aaactgaatc ttcacccgcg cgccagtaac
    13741 agcatcaata cgttgccgtt accggttgaa cgcgtccgcc tgctgattgg cccggaaggc
    13801 ggtttatcgg cagatgaaat tgccatgact gcccgctatc aatttactga tatcctgttg
    13861 ggacctcgcg ttttgcgtac agagacaact gcgctcaccg ccattaccgc gctacaagta
    13921 cgatttggcg atttgggcta acggagaaga ataatgatca agctcggcat cgtgatggac
    13981 cccatcgcaa acatcaacat caagaaagat tccagtttcg ctatgttgct ggaagcacag
    14041 cgtcgtggtt acgaacttca ctatatggag atgggcgatc tgtatctgat caatggcgaa
    14101 gcccgcgccc atacccgcac gctgaacgtg aagcagaact acgaagagtg gttttcgttc
    14161 gtcggtgaac aggatctgcc gctggccgat ctcgatgtga tcctgatgcg taaagacccg
    14221 ccgtttgata ccgagtttat ctacgcgacc tatattctgg aacgtgccga agagaaaggg
    14281 acgctgatcg ttaacaagcc gcagagcctg cgcgactgta acgagaaact gtttaccgcc
    14341 tggttctctg acttaacgcc agaaacgctg gttacgcgca ataaagcgca gctaaaagcg
    14401 ttctgggaga aacacagcga catcattctt aagccgctgg acggtatggg cggcgcgtcg
    14461 attttccgcg tgaaagaagg cgatccaaac ctcggcgtga ttgccgaaac cctgactgag
    14521 aatggcactc gctactgcat ggcacaaaat tacctgccag ccattaaaga tggcgacaaa
    14581 cgcgtgctgg tggtggatgg cgagccggta ccgtactgcc tggcgcgtat tccgcagggg
    14641 ggcgaaaccc gtggcaatct ggctgccggt ggtcgcggtg aacctcgtcc gctgacggaa
    14701 agtgactgga aaatcgcccg tcagatcggg ccgacgctga aagaaaaagg gctgattttt
    14761 gttggtctgg atatcatcgg cgaccgtctg actgaaatta acgtcaccag cccaacctgt
    14821 attcgtgaga ttgaagcaga gtttccggtg tcgatcaccg gaatgttaat ggatgccatc
    14881 gaagcacgtt tacagcagca gtaacccacc ttagcgagaa ggatctcgtt gagactctga
    14941 gtgacagcgc ccttctttcc acgcatactg ggcgctgttg cttttttgaa ccaggaaaca
    15001 gaacctctga caatgaattt acagcatcac tttcttattg ccatgcctgc tctccaggat
    15061 ccgattttcc gtcgttccgt ggtctacatt tgcgaacata ataccaatgg tgcaatgggg
    15121 atcatcgtca acaagccgct ggaaaatctc aaaattgaag ggattctgga aaagctgaag
    15181 atcacgccgg agccgcgtga tgaatcaatc cgtctggata aaccggttat gctcggcggt
    15241 ccgctggctg aagatcgcgg gtttattttg catactccgc cctccaattt tgcttccagc
    15301 attcgcattt cagacaacac agtgatgacc acttcccgcg atgtgctgga aacgctcggc
    15361 accgataaac aaccgtctga cgtattggtg gctctaggtt atgcctcctg ggaaaaaggt
    15421 caactggaac aagaaattct cgataacgcg tggctaacgg ccccggcgga tctgaatatt
    15481 ctgttcaaaa cgccgattgc cgaccgctgg cgcgaggcgg caaaactgat tggtgtggat
    15541 attctcacca tgcctggtgt ggcagggcac gcctgatgag tggaacctta ctcgccttcg
    15601 acttcggcac caaaagcatt ggcgtagcgg tcggccaacg cattaccggc actgctcgcc
    15661 ctttgcctgc aattaaagca caggacggta cgccggactg gaacctcatc gaacgtttac
    15721 tgaaagagtg gcagccggac gaaatcatcg tcggtttgcc gctgaatatg gacggcaccg
    15781 agcaaccatt gactgccaga gcgcgtaaat ttgccaaccg tattcatggt cgtttcggtg
    15841 ttgaagtaaa gctccatgac gaacgtctta gcaccgtgga agcccgttcc ggtctgtttg
    15901 aacagggcgg ctatcgggcg ctcaacaaag gcaaagtcga ctctgcctct gcggttatta
    15961 ttctcgaaag ctatttcgag cagggatatt aaggcgattt aaaagcctgg cggatagtcg
    16021 ttaccgccat cttgccggat gcggcgtgaa cgcctatccg gcctacatgt ggcagcgatt
    16081 gtaggtctga taagacgcgc aaacgtctca tcagacgttt attgcctttg taggcccgat
    16141 aagcttgcac atcgggcatg gcaacgtcac aaacgccctt cccccactcg ctgctgaaaa
    16201 ctctgctgaa acgttatcat ccccacctgc tgcccggttt gaataacatg cggtaactgg
    16261 tgggttttcc cttcgcgaat caaattcccc accgcgggag tgttaatcaa taattcaaac
    16321 agcgccacgc gtccttcctg tttatccact tccagctttt gtgacaacac ggcccgtaaa
    16381 ctacctgcca gttgattacg tacgggatct ttttcctgcg ccggaaatga atccaccagt
    16441 cgctcaactg cctgcgcggc accacgcgta tgtaatgttg ccagcaccaa atgcccggtt
    16501 tctgccgccg tcagtgccag acggattgtc tcgctgtcac gcagctctcc gagcaaaatc
    16561 acatcaggat cttcccgcaa tgcggcccgc aatcccgatg cgaacgtcat acagtgcaaa
    16621 ccaatttccc gcttctggat caaacatcgc tggctggcat agatatattc aacaggatct
    16681 tccagcgtca gaatatgcgc atcggcatgt tgattgagat agccaaccat cgccgccagc
    16741 gtggtagatt tgctgctccc cgtcgccccc gtcaccagaa tcaggccatt ctcactcttg
    16801 agtaattccg gcaataccgt tggtgcgcca agctgttcga gctgcgggca gtgcgaaggt
    16861 aacagccgta acgccagcga aatgccatgc cgttgcgcaa atgcgctgcc acgcaatcgc
    16921 tggttttccg ccagcgacac ggcaaaatcc agctggccat tttccagcaa tattgcccgc
    16981 tgatcgtcat ccagccactc ccgcagtagc tcttcgacgt ccggcgcctc aaacggcgca
    17041 gcttccatta gcccgcgaat gcgccatcgt gcgggccagg cgctgcacag gtgtagatcc
    17101 gagacgttat gctttacact aagggccaca atttcttcca tattcatact aagatcctcg
    17161 gaaaatgaac gatattgcgc ataacctggc acaggtccgg gacaaaatct cagcggctgc
    17221 aacgcgttgc ggccgttctc cagaagaaat tacgctgctt gcagtcagta aaacaaaacc
    17281 tgcgagcgcc atcgcagaag ccattgatgc cgggcagcgt caatttggtg aaaactacgt
    17341 tcaggaaggg gtagataaaa ttcgccactt tcaggaactg ggcgtaacag gattagaatg
    17401 gcattttatt ggcccgttgc agtctaataa aagccgcctg gtggcagagc atttcgactg
    17461 gtgtcatacc atcgaccgtt tgcgcattgc tacccgcctc aacgatcagc gcccggcaga
    17521 acttccccct cttaacgtac tgattcaaat taatattagt gatgaaaaca gtaagtccgg
    17581 gattcaactg gcagaactgg acgagctggc agcggcggtc gctgaactac cgcgtttacg
    17641 tctgcgcggg ctgatggcaa tccctgcgcc tgagtcagaa tatgtaaggc agtttgaagt
    17701 tgcacgccaa atggctgtag catttgccgg actgaaaacg cgctacccgc atatcgacac
    17761 gctctctctg ggaatgtcgg acgatatgga agccgccatt gcggcaggta gcacgatggt
    17821 tcgtatcggc actgcaattt ttggtgcgcg tgattactct aaaaaataag gaattaaagg
    17881 aacgccatga atacgttgac tttcctgctt tcaacggtca ttgagctgta taccatggtg
    17941 ctgttattac gcatctggat gcagtgggct cattgtgatt tttacaaccc cttctcacag
    18001 tttgtagtga aggtaacgca gccaattatc gggccactgc gccgcgttat tccggcaatg
    18061 ggccccattg acagcgcctc gctgctggtt gcctatattc tcagttttat caaagccatc
    18121 gtgctgttta aagtggtgac cttcctgcca atcatctgga ttgctggttt actgattctg
    18181 ctgaaaacca tcggcctgct gattttctgg gtcctgctgg tgatggcgat tatgagctgg
    18241 gtaagccagg gtcgtagtcc gattgaatac gtgctgattc agctggccga tccgctgctg
    18301 cgcccgattc gccgcctgct accggcaatg ggtgggattg atttctcgcc gatgatcctc
    18361 gttctgctgc tgtatgtcat caatatgggt gtcgcagaag tattacaggc aaccggaaac
    18421 atgctgctgc cggggctgtg gatggcgtta tgagtgccgt aacagttaat gatgacggtc
    18481 tggttttacg gctctatatt cagccgaaag ccagccgtga ttctattgtc ggtttacatg
    18541 gcgacgaagt taaagtcgcc attaccgcgc cgccggttga cggccagggc aacagtcatc
    18601 tggtgaagtt tctcggtaag caattccggg ttgccaaaag ccaggtggtg attgaaaaag
    18661 gcgaacttgg ccgccacaaa caaattaaaa tcattaatcc gcaacaaatc ccgccagaaa
    18721 tcgtggcgtt aattaattag gtatcctatg caaaaagttg tcctcgcaac cggcaatgcc
    18781 ggtaaagtgc gtgagctggc gtcgctgctt agcgacttcg gtcttgatat cgtggcccaa
    18841 acagacctcg gcgtagattc cgctgaagaa accggcctga cctttatcga aaacgcgatt
    18901 ctgaaagcgc gctatgcggc aaaagtgacc ggtttaccgg caattgccga cgactctggt
    18961 ctggcggtag atgcgctggg tggcgcgccg ggtatttact ccgcgcgtta ttccggtgaa
    19021 gacgcgaccg atcaaaagaa tctgcaaaaa ctgttggaaa cactgaaaga cgtaccggac
    19081 gaccaacgtc aggcgcgttt ccactgcgtg ctggtatatc tgcgtcacgc ggaagatccg
    19141 actccgctgg tatgccacgg cagctggccg ggcgtgatta ctcgcgaacc agcgggcact
    19201 ggtggctttg gttatgatcc aatcttcttc gtaccttccg aagggaaaac cgccgccgaa
    19261 ctgacccgcg aagaaaagag cgccatttcc caccgtggtc aggcgttgaa actgctgctg
    19321 gacgctttac gtaatggtta aattaccgcc gctgagtctc tacattcaca tcccgtggtg
    19381 cgtgcagaaa tgcccgtact gcgatttcaa ctctcacgcg ttgaaaggag aagtgccgca
    19441 cgacgattat gttcagcatc tgctttgcga tctggacaat gatgtggctt acgctcaggg
    19501 ccgtgaagta aagacaatct ttattggcgg tggtacgccg agcctgcttt ccggcccggc
    19561 gatgcaaacg ctgctggacg gcgtgcgtgc gcgtttgccg ctggcagcgg atgcagaaat
    19621 tactatggaa gcgaaccctg gaacggtaga agccgatcgc tttgtcgatt atcagcgtgc
    19681 tggtgtgaac cgcatctcta ttggtgtgca gagttttagc gaagaaaagc tgaaacgact
    19741 tgggcgtatt catggcccgc aagaagcgaa acgcgcggca aagctggcga gcgggctggg
    19801 gctgcgtagc tttaacctcg atttgatgca tggactaccg gatcaatcac tggaagaggc
    19861 gcttggcgat ctacgccagg ccattgaact gaatccgccg catctttcct ggtatcaact
    19921 gaccatcgaa cccaatacgc tgtttggttc gcgaccaccg gtgctgccgg acgatgacgc
    19981 gctgtgggat attttcgaac aggggcatca gttattaacc gcagcgggtt atcagcaata
    20041 tgaaacttcc gcttacgcca aacccggtta tcagtgccag cacaatctca actactggcg
    20101 atttggtgac tacatcggta ttggctgcgg cgcgcacggc aaagtcacct tcccggatgg
    20161 gcgcattctg cgtaccacta aaacgcgtca tcctcgtggt tttatgcaag gaaggtatct
    20221 ggaaagccag cgtgatgtcg aagccgcaga taagccgttt gagttcttta tgaatcgctt
    20281 ccgtctgctg gaggccgcgc cgcgcgtgga gtttattgcg tataccgggc tttgcgaaga
    20341 tgtgattcgc ccacagttag acgaggcgat tgcccagggt tatctcaccg aatgtgcgga
    20401 ttactggcag ataaccgagc acggtaaatt attcttaaac tcacttcttg aattattcct
    20461 cgctgagtaa tcattaatgc gcctgcgggc gcattaatca cttcttttcc ccccgttaat
    20521 taacttcggc aaaatatatt tcttactgaa taaacatcga tgatatttat tagatatatc
    20581 tagtgaatat ttactcatca tttattgatt ataaacatgt acaacctggc gcacaaattt
    20641 aatatttaat ttctgacttt atttcaagtt gtgataaaca tagacaagtt gtcagatagg
    20701 ttaaccaatt tgtcgcacaa agcactgata agcacaatta gatatcaacc aatttccatc
    20761 cttcaacagg gagttttttt atgaaaatga ggtcatttac ccgctcatta gtctgcgcat
    20821 ccttgctggc tctggttagc actggcgtaa acgccgcaga aaaagtgacg ttaaagttag
    20881 cacacaacct ggaaagaagt catgttgtac atcagagttt tgaagagctg gcgaaagagg
    20941 taaaacagct gtcagaaggt aatatggtga tccgtattta tcccagcagc cagatgggta
    21001 atgcgcgaga aactatggaa ttgttgcaaa atggcgcact ggatatgacc aaaggttccg
    21061 ccagtgatct ggagtctttt gataatattt atgctatcta taatttgccg ttcttattta
    21121 aagatcaggc tcacttcaac aaagttgttt ttggtgaagt tggtaaagaa attatggact
    21181 caacaaaaga caaaggcttt tttgctttat cagcttatgt agcgggtaca cgcagtttct
    21241 atgcgaaaaa accgattact aagccagaag atcttaaagg gctgaaaatt cgtgttcagc
    21301 caagtccgac caccatcaaa atgattgaac tgatgggtgg ttcacccact ccaatttcat
    21361 ttggcgaagt ttatactgcg atgcagcaag gtgtagtaga tggcgcagaa aataacgtgc
    21421 cttcctgggt gcagactcga catattgaaa ttgccaaagt tttttctgaa gatgaacacg
    21481 cctcaattcc tgactttttg gttatctcca ccaaaacatg gaataaatta accccagaac
    21541 agaaacaaat cctcgaaaca gcagcgaaga aatccgaagc ttatcaacaa aaattgtggg
    21601 aaaaaattga cgctgacaca cgtgctcagg cgaaagctat ggg
//
LOCUS       DAJVQG010000065        21643 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJVQG010000065 DAJVQG010000000
VERSION     DAJVQG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.065"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="OQM83_002158"
     CDS             complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="OQM83_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCS1520421.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1345..2997
                     /gene="ushA"
                     /locus_tag="OQM83_002159"
     CDS             1345..2997
                     /gene="ushA"
                     /locus_tag="OQM83_002159"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCS1520422.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTREDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="OQM83_002160"
     CDS             complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="OQM83_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCS1520423.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3717..4511)
                     /locus_tag="OQM83_002161"
     CDS             complement(3717..4511)
                     /locus_tag="OQM83_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCS1520424.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4649..4990
                     /locus_tag="OQM83_002162"
     CDS             4649..4990
                     /locus_tag="OQM83_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCS1520425.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5205..7709)
                     /gene="copA"
                     /locus_tag="OQM83_002163"
     CDS             complement(5205..7709)
                     /gene="copA"
                     /locus_tag="OQM83_002163"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCS1520426.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTIVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7971..8903
                     /gene="glsA"
                     /locus_tag="OQM83_002164"
     CDS             7971..8903
                     /gene="glsA"
                     /locus_tag="OQM83_002164"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCS1520427.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8906..10198
                     /locus_tag="OQM83_002165"
     CDS             8906..10198
                     /locus_tag="OQM83_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1520428.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10323..10730
                     /gene="cueR"
                     /locus_tag="OQM83_002166"
     CDS             10323..10730
                     /gene="cueR"
                     /locus_tag="OQM83_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCS1520429.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10731..11189)
                     /locus_tag="OQM83_002167"
     CDS             complement(10731..11189)
                     /locus_tag="OQM83_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCS1520430.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11186..12103)
                     /locus_tag="OQM83_002168"
     CDS             complement(11186..12103)
                     /locus_tag="OQM83_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCS1520431.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12249..12926
                     /gene="fetA"
                     /locus_tag="OQM83_002169"
     CDS             12249..12926
                     /gene="fetA"
                     /locus_tag="OQM83_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCS1520432.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12913..13692
                     /gene="fetB"
                     /locus_tag="OQM83_002170"
     CDS             12913..13692
                     /gene="fetB"
                     /locus_tag="OQM83_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCS1520433.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="OQM83_002171"
     CDS             complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="OQM83_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCS1520434.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="OQM83_002172"
     CDS             complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="OQM83_002172"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCS1520435.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="OQM83_002173"
     CDS             complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="OQM83_002173"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HCS1520436.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16063..16749
                     /locus_tag="OQM83_002174"
     CDS             16063..16749
                     /locus_tag="OQM83_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1520437.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16746..19160
                     /locus_tag="OQM83_002175"
     CDS             16746..19160
                     /locus_tag="OQM83_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1520438.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="OQM83_002176"
     CDS             complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="OQM83_002176"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCS1520439.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20557..21483)
                     /gene="allS"
                     /locus_tag="OQM83_002177"
                     /pseudo
     CDS             complement(20557..21483)
                     /gene="allS"
                     /locus_tag="OQM83_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5015 a         5684 c         5799 g         5145 t
ORIGIN      
        1 tgatcggcga taagccccag aactgccgct cccagacctc ccatgccaaa agcaaaaccg
       61 aaaaagagtc cagaaaccat accgatacgt cccggaagta gctcctgagc gtagaccaga
      121 atggcagaga atgccgaagc gaggataaat ccaataatca ccgttaaaac ccccgtccag
      181 tgcaggctgg cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat agagccccaa
      241 atcacatatt tccgtccaat tttatcccct acaggcccgc cgatcaccgt acctgccgca
      301 acggcaaaca ggaaggcaaa cagatgaagc tgggcattct ggatagataa tccgaatttt
      361 tgcatcagat aaaaggtgta atagctgctg atgctcgcca tatagaaata tttcgagaaa
      421 atgaggatta acagaatgct gactgccagt accactttgt tgcgcggcag tggattgatt
      481 atcgtcgctt tgggttttcc tttattcatt cggtgctgtg ccgagtacca acggctaatt
      541 tgcgccaaca ccacgatcgc cagcagtgcc gcaagcacaa accaggcaac gttgcctttg
      601 ccataaggcg cgataatcac cgccgccagc aagggtccca gggaactgcc aaagttgccg
      661 ccgacctgaa agatagattg cgccaggcca tgccgcccgc cggaagccat acgggctacg
      721 cgagaagatt ccggatgaaa gaccgatgaa ccggtaccga ccagcgccgc cgccagcaga
      781 actgcgccaa aactgcccgc cagcgcaagc agcaccagac cacttaaggt aaagcacatg
      841 ccaattggca acgaccacgg catcggatat ttatcggtcc agtagccgac cactggttgc
      901 agtagcgaag aggcgagctg gaaggtgagg gttatcatgc caatctgcat aaatgtcaga
      961 gaaaattctg actgaagcag cggataaatc gccagaatca gcgattggat catgtcgttc
     1021 agcagatgtg agaggctgat agcacctaaa ataccaaacg atgttcgggc cttggtcgtt
     1081 gacgcagccg cgcccgccac aggctggggt tgttcactca ttgccatagg aaagtcactt
     1141 tttcagggtt gcgatgtaaa gaatgatctt atttgtgatt attacccgac taacatacct
     1201 gtatgcgtcg tctgaaggaa gtctcaacgc cgaatacaga atttctaatc tggatgcaga
     1261 tttatcttca ccggacgcag acttgtctat gatgtcgcgt catactattt ttcaacacgt
     1321 tgaaatcagg tcagggagag aagtatgaaa ttattgcagc ggggcgtggc gttagcgctg
     1381 ttaaccacat ttacactggc gagtgaaact gctctggcgt atgagcagga taaaacctac
     1441 aaaattacag ttctgcatac caatgatcat catgggcatt tttggcgcaa tgaatatggc
     1501 gaatatggtc tggcggcgca aaaaacgctg gtggatggta tccgcaaaga ggttgcggct
     1561 gaaggcggta gcgtgctgct actttccggt ggcgacatta acactggcgt gcccgagtct
     1621 gacttacagg atgccgaacc tgattttcgc ggtatgaatc tggtgggcta tgacgcgatg
     1681 gcgatcggta atcatgaatt tgataatccg ctcaccgtat tacgccagca ggaaaagtgg
     1741 gccaagttcc cgttgctttc cgcgaatatc taccagaaaa gtactggcga gcgcctgttt
     1801 aaaccgtggg cgctgtttaa gcgtcaggat ctgaaaattg ccgttattgg gctgacaacc
     1861 gatgacacag caaaaattgg taacccggaa tacttcactg atatcgaatt tcgtaagccc
     1921 gccgatgaag cgaagctggt gattcaggag ctgcaacaga cagaaaagcc agacattatt
     1981 atcgcggcga cccatatggg gcattacgat aatggtgagc acggctctaa cgcaccgggc
     2041 gatgtggaga tggcacgcgc gctgcctgcc ggatcgctgg cgatgatcgt cggtggtcac
     2101 tcgcaagatc cggtctgcat ggcggcagaa aacaaaaaac aggtcgatta cgtgccgggt
     2161 acgccatgca aaccagatca acaaaacggc atctggattg tgcaggcgca tgagtggggc
     2221 aaatacgtgg gacgggctga ttttgagttt cgtaatggcg aaatgaaaat ggttaactac
     2281 cagctgattc cggtgaacct gaagaagaaa gtgaccaggg aagacgggaa aagcgagcgc
     2341 gtgctgtaca cccctgaaat cgctgaaaac cagcaaatga tctcgctgtt atcgccgttc
     2401 cagaacaaag gcaaagcgca gttggaagtg aaaataggcg aaaccaatgg tcgtctggaa
     2461 ggcgatcgtg acaaagtgcg ttttgtacag accaatatgg ggcggttgat tctggcagcc
     2521 caaatggatc gcactggtgc cgactttgcg gtgatgagtg gaggaggaat tcgtgattct
     2581 atcgaagcag gcgatatcag ctataaaaac gtactgaaag tgcagccatt cggcaatgtg
     2641 gtggtgtatg ccgacatgat cggtaaagag gtgattgatt acctgaccgc cgtcgcacaa
     2701 atgaagccag attcaggtgc ctacccgcaa tttgccaacg ttagctttgt ggcgaaagac
     2761 ggcaaactca acgacctgaa aatcaaaggc gaaccggtcg atccggcgaa aacttaccgt
     2821 atggcgacat taaacttcaa tgccaccggc ggtgatggct atccgcgcct tgataacaaa
     2881 ccgggctatg tgaataccgg ctttattgat gccgaagtgc tgaaagcgta tatccagaaa
     2941 agctcgccgc tggatgtgag tgtttatgaa ccgaaaggtg aggtgagctg gcagtaatcc
     3001 gaaagtgccg gatatttgca tccggcacaa tgcttaatcg cggcgggcga tatcagcaaa
     3061 tttggcatcg aggatcttcg ccagatcgcc tgccgccagt tcgatatcca gtccgcgctt
     3121 gccgccagaa acataaatag tggcaaattc ttgtgcgggg gcgtcgataa tcgtcggcag
     3181 acgttttttc tgccccaatg ggctaatccc cccaaccagg tatcccgtcg aacgctgcgc
     3241 ggccatcgga tcggccatct caactttctt ggcacccagc gcttttgcta cttttttaag
     3301 atccagttga cctgcgaccg gcgtaacggc cactgccaga tgtttcatat caccgttcac
     3361 tgccaccagt agcgttttgt agacttgatc cggattcaaa cctaattttt tgacgacttc
     3421 atcgccaaaa ttggtttcag ccggatcgtg ctcgtaggta tggatttgaa acgaaatctt
     3481 gtttttttcg agtaatttaa ctgcgggtgt catggtaatt ttcctgcctt aaacaaaaaa
     3541 acgaagcaag catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc ctaagagtat
     3601 tggcaggatg gtgagattga gcgacaatcg agttacaccg tcgcttaaag tgacggcata
     3661 ataataaaaa aatgaaattc ctctttgacg ggccaatagc gatattggcc atttttttag
     3721 cgcaacattt gcggcaaatt cccttctcca tacaggtgta gtgcaccgac cgcgaccaca
     3781 tatcgccccg gcggcatggc gcgtaattta tcccgccagg cgagatttcg ctgatgcatc
     3841 agcacatcgt acagcgactg actgaacgta ttgggcagcg ttatatcatt attttgcgga
     3901 ggtgcattca gccaccagct catcatttgt tgcagcaacc gtgcgttggt atgccagtgg
     3961 gtcagcgtat cgtccagcag cgccagtcct ttgtcaggga gctggagcaa catggtaatc
     4021 tggttttcag ccccttccag ttcaatcacg ggtttatgtt gttgcttcgc cgcctgcaat
     4081 aactggtaat cgataccgta ttctgcccgc agccccagtt tttgtgcctg cgtcgcctga
     4141 agaaccatcg cgatttgcca cagcggttga gtagaaaaga gcgacggaga aatgcccatc
     4201 tcctggctaa tgtgctgcag attttggagt tgctcctcgt taatgcgctc ttccagcgcc
     4261 tcgcaagtag gcaaattagc aaaaggcgta tcgctggtgg aaacatccgc ctcgacgatc
     4321 agcgcatcgg cgtttttgag ctttttgagc aaacgggtgg gcaggggagc catatcgtgg
     4381 ctacccatat gaatactgcc aatcagatga aaatggcgat tgccgggaag ggtgatatcg
     4441 atggcaggcc aggtgtaatg attaccgcgc agcgcggccc aaagcgtttt tacccggtac
     4501 aacagatcca tacgacctcc ctttgtgaaa tatcatgcta gcgcgcggtg agggatggcg
     4561 caaatggagt tggggacttg tttaatgtgt ttgtatgatt cagtatgttc ttgcatcgct
     4621 attcacaggg aagcaacagt taaaaaccat gaaacaggca acaagaaaac cgacgacacc
     4681 cggagatatt cttctctatg aatatctgga accgctcgat ttgaaaatca atgagttagc
     4741 agagttgctg catgttcatc gtaatagcgt cagtgcactg atcaataaca atcgtaaact
     4801 caccactgag atggcattcc gtctggcgaa agtttttgat accacagtcg attttaggct
     4861 aaacctccag gcggcagttg atctttggga agttgaaaac aacatgcgca cccaggaaga
     4921 attgggacgg attgaaacag tggctgaata tttggcacgc cgtgaagagc gtgcaaaaaa
     4981 ggtcgcgtaa ggccaaaaca tcgcttcatc tcgtaggccg gataaggcgt tcacgccgca
     5041 tccggcatcc gagcaccatt gcctgatgcg acgcttaccc gtcttatcag gcctacaaac
     5101 ctgtgcctga accgtaggcc ggataaggcg tttacgccgc atccggcagt catgcgtcga
     5161 tgccaaatgc gccgccctaa agcagcgcat ccgcaatgac gtacttattc cttcggttta
     5221 aaccgcagca accggttggc gttactcact acggtaatcg acgagagcgc cattgccgct
     5281 ccggcaacta ccgggttaag cagtgttccc gtaaacggcc acaaaatacc ggcggcgacc
     5341 ggaataccga tgctgttgta gataaaggca ccgagcaggt tctgcttcat gttgcgcagc
     5401 gttgcgcggg aaatcgcgag cgcatccgca acgcccatca ggctatggcg catcagggta
     5461 atcgccgcgg tttcaatggc aacatcactg ccgccaccca tcgcaatgcc gacatccgcc
     5521 tgggccagcg ctggcgcgtc gttaatgccg tcgcccacca ttgccacctg acgtccttca
     5581 ctttgcagac gtttgatcgc ttcggcttta ccgtccggca gcactccggc aatcacttca
     5641 tcaattccag cttctttggc gatcgcattg gcggtggttg ggttatcccc ggtcaacatc
     5701 accagacgat atcccgtttt atgcaggcgt tgcagcgccg ccacgctatc actacgcaac
     5761 ggatcgcgta cagccagcag ggctactgct ttcccgtcaa tcgccagcag cacaggcgtt
     5821 gctccttgcg atgcctgagc agtaatctcc gcttcgatag ctttggtacc aacctgttgc
     5881 tcatttaaca gcgcctgatt gcccagcaat aacgcatgac cttcagcttc accgctcacg
     5941 cccagcccgc gcaatgtgcg gaaaccgttg acctgcggca gctgcatatc acctgcttta
     6001 tcgaggatcg cccgtgccag cggatggctg gaaccttgct ccagtgccgc cgccagacgc
     6061 aatgcctgcg cttcatcaac atcagcaaac gttttcactg cgacaacctg cggcttccct
     6121 tcggtcagcg tcccggtttt atcgaacacg atggtatcga gtgtactggc gcgttgcagc
     6181 gcgtcggcgt cccgcaccag cacgccaaac tcagccgccc gcccgacgcc ggaaataatc
     6241 gacatcggcg tcgccagccc cagcgcacac ggacaggcga taatcagtac cgtggtggca
     6301 atcaccaggg tataaacaat ttgcggtgcc ggaccaaaga agtaccagat tgccgcactg
     6361 accagcgcaa tcaccaccac cacaggtaca aatacggctg agattttatc cgccagctga
     6421 ccgatttctg gcttgctgct ctgggcctgg cgcaccatgc gaatgatccg tgacagcgtg
     6481 gtatggctgc caaccgcact ggcacgaaac agcacactgc cgtcctgtac cactgtcccg
     6541 gcatggacgc tttcgccttc gcctttttgc tgtgggattg gttcgcccgt cagcatcgct
     6601 tcatccagcc atgcttcgcc ctgggtaatc tcgccatcta ccggcacgcg atcgccggtc
     6661 gtcaggcgca gtaacatccc tggctgaact tccgccagcg gcacgctttt ttcaccatct
     6721 gcggtgacca ctcgcgccgt cggtggggtt aaatcgagta atttctccag cgccttagaa
     6781 gaacgctgac gtgcgcgcgc ttccagcata tggccgagat taatcagacc gataatcatc
     6841 gcactggctt catagtaaag atgccgcgct tccatcggga accactgcgg ccacaggttg
     6901 acgctcatcg aatagagcca cgccacaccc gttcccagcg ccaccagcgt atccatcgtc
     6961 gctgcaccgt tcagcaggct tttccatgca ctgcggtaaa aatggccgcc ggcgaaaacc
     7021 atcactgcca gggttatcag gccgataact aaccacaagc tgcggttgtc agcggtgacc
     7081 atcatgttat cgccgatcat cccccagacc attaccggga tacccacagc cagtgcaaca
     7141 attgcctgcc agcggaagcg cttcatcgta gctacggcgg tttcttgctg gcgctcgcgg
     7201 cgtttagcgt catcttcaat cgcttccgca ccgtagcccg ctttttccac cgcctgcact
     7261 aaatcttgtg gggaggcact gcccatcacc agcgcagtac gctccgccag gtttacccgt
     7321 gcctgagtga cgcccggtac gctttgcagc gcattttgta cgcgggtgac acagctggcg
     7381 cagctcatgc cgctcagcag caactgctgg ctgtcatcgt catcggcggt cgctgccgga
     7441 agcgcctcag aaaccgctgt cagtgcttcc gacgggattg atgactccgc cagcggttta
     7501 gcctttgggt ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc gatcagctgt
     7561 tctgcactgg cagtcccggt aacgtgcgct tcagtgatag acacatccgc ctgctcaaca
     7621 tccgggcgct gttcaagact ttctttcacg cgtttaacgc agtgaccgca ggacaggccg
     7681 tccagggtca ggtcgatagt ttgtgacata aaacactcct ttaagacagt tttgactggc
     7741 tgtgataaag gttaaacctt ccagcaaggg gaaggtcaag aaattaataa accaggcggg
     7801 taaaagtccg taaagattaa aaaatcggct cgatttgcat caggattaga catttatttc
     7861 tttgttttcc tgtagttaag ttgcgggtgc taagttaaat caggatgcct gaaaatcggc
     7921 accggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag atgttagatg
     7981 caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca cttaacggcg
     8041 gacaaaatgc cgattacatt ccctttctgg cgaatgtacc aggtcaactg gcggcagtgg
     8101 ctatcgtgac ctgcgatggc aacgtctata gtgcgggtga cagtgattac cgctttgcac
     8161 tggaatccat ctctaaagtc tgtacgttag cccttgcgtt agaagatgtc ggcccgcagg
     8221 cggtacagga caaaattggc gctgacccga ccggattgcc ctttaactca gttatcgcct
     8281 tagagttgca tggcggcaaa ccgctgtcgc cactggtaaa tgctggcgct attgccacca
     8341 ccagcctgat taacgctgaa aatgttgaac aacgctggca gcgaatttta catatccaac
     8401 agcaactggc tggtgagcag gtagcgctct ctgacgaagt caaccagtcg gaacaaacaa
     8461 ccaacttcca taaccgggcc attgcctggc tgctgtactc cgccggatat ctctattgtg
     8521 atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc tacgctcatc aatactgttg
     8581 aactggcaac gcttggcgcg acgctggcgg cgggtggtgt gaatccgttg acgcataaac
     8641 gcgttcttca ggccgacaac gtgccgtaca ttctggccga aatgatgatg gaagggctgt
     8701 atggtcgctc cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc ggtgtaggtg
     8761 gcggtattct ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca ccaccgctgg
     8821 acgaagaagg caacagtgtt cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg
     8881 gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg gtaacaaacc
     8941 cctcggtcta tggaacgtcg tttccatcgg tattggggca atggtggggg cggggatctt
     9001 cgcgctgctg gggcaggctg cgttgctaat ggaagcctcg acctgggtcg cctttgcttt
     9061 tggcggtatt gtggcgatgt tttccggtta tgcctatgcg cgtctggggg cgagctatcc
     9121 cagcaatggc ggtattatcg acttttttcg tcgcggatta ggcaacggcg tcttttcgct
     9181 agcgctctcg ttgctgtacc tgttgacgct ggcggtgagc atcgccatgg ttgcccgtgc
     9241 ttttggcgct tatgccgtgc agtttttgca tgaaggcagc caggaggagc accttatttt
     9301 gctctacgcg ttggggatca ttgcggtgat gacgcttttt aactccttaa gcaaccatgc
     9361 ggtagggcgg ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt tattgctgat
     9421 tattgccggt gtctggtcgc tgcaaccggc gcatatttcc gtctctgcgc cccccagctc
     9481 cggtgcgttc ttctcctgta ttgggataac cttccttgcc tatgcgggct ttggcatgat
     9541 ggcgaacgcg gcggataaag tgaaagatcc gcaggtcatt atgccacggg cgtttctggt
     9601 ggcgattggc gttaccacgt tgctttatat ctcgctggcg ctggttttgc ttagcgatgt
     9661 atcggcatta gagttagaaa aatatgctga taccgccgta gcgcaggctg cttctccgct
     9721 gctcgggcat gtgggttatg tgatcgtcgt catcggcgct ttactggcga cggcttcagc
     9781 cattaacgcg aacctgttcg ccgtgtttaa catcatggac aacatgggca gcgaacgcga
     9841 actgccgaag ctaatgaata aatccctgtg gcggcagagt acctggggca acattattgt
     9901 cgtggtgttg attatgctga tgacggcggc gctaaattta ggctcactcg ccagtgttgc
     9961 cagcgccacc tttttgattt gctacctggc ggtgtttgtg gtggcgatcc gcctgcgtca
    10021 tgatattcac gcctcgttgc cgattcttat cgttggtacg ttggtgatgt tgttggtgat
    10081 cgttggcttt atctacagtc tgtggtccca gggtagccgt gcgttgatat ggattattgg
    10141 ctcactctta ctcagcctta ttgtggcaat ggtcatgaag cgcaataaaa ccgtataaca
    10201 tctctctgtg cgcagtactt cctgtattat tgtggtggcg gtcgatattc gcactggcaa
    10261 aaaaacgtgc ttgaatatct gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa
    10321 cgatgaacat cagcgatgta gcaaaaatta ccggcctgac cagcaaagcc attcgcttct
    10381 atgaagagaa ggggctggtg acgccgccga tgcgcagcga aaacggttat cgcacctaca
    10441 cgcagcagca tctcaacgaa ctgaccttac tgcgccaggc acggcaggtg ggctttaacc
    10501 tggaagagag cggcgagctg gtgaatctgt ttaacgaccc gcagcggcac agcgccgacg
    10561 tcaaacggcg cacgctggag aaggtggcgg agatcgaacg acacattgag gagctgcaat
    10621 ccatgcgcga ccagctgctg gcactggcga atgcctgccc tggcgatgac agcgccgact
    10681 gcccgattat cgaaaatctc tccggctgct gtcatcatcg ggcagggtga ttaagacgag
    10741 acggctcgga tatgtagggt tatcccttct atcgcaatga cttcaacatg cgtacctgca
    10801 ccgagatcct cgctggcgct gacaggccat gaactgtcac cgacgcgcat atgaccgcgc
    10861 ccgttgacca gcggagattc cagcacaaaa cgtcggccaa tcagctgctg cccgcgctgg
    10921 tttaaatgac tatcgctgtg cttttgttcg cgcacccgcc gcgacaacca tttccaccac
    10981 agccaggcgg cgagcagcgt caggatggca aacattaccc cttgccactc ccaacccagc
    11041 ggcaccagcc agaccaccag gccggtaatc accgctgcca cgccgctcca caacaaataa
    11101 ccatttccgc ccagcatctc ggctgccagc agcaaaccgc cgagactgag ccagaaaata
    11161 tgtggatgaa cgaccattaa ctccatcatg gctgagtccg tttgttggcg ctgtctttca
    11221 ccagctcggc aatcccggca atcgacccca tcaggctgct ggcctctaat ggcatcatca
    11281 ctactttgct gttactggag gtaccgatct gctgtaacgc ttcggtgtat ttctgcgcta
    11341 cgaagtagtt caccgcctga atatcaccgg aggcgatagc ttcagacacc attttggtgg
    11401 cgcgggcttc tgcttcggcg gaacgttcac gcgcttcagc ctgtaaaaac gccgactgac
    11461 gttcgccttc cgctttcagg atttgcgact gtttttcacc ttcggctttg aggatttccg
    11521 cctgacggat cccttccgct tcaagaatgt aagcgcgttt ggtacgttcc gctttcatct
    11581 gcgcgttcat tgaagagata agctctgccg gtgggcgcac gtcgcgaatt tcaatacggg
    11641 tgactttaat cccccacggg ttggtggctt catcgacaat acgcagcagg cgtgagttga
    11701 tgctgtcgcg ctgagagagc atttcgtcaa gttccattga acccaacacg gtacggatgt
    11761 tagtcatggt caggttgatg atcgccagct ccagattgct gacttcataa gccgcgcgcg
    11821 gcgcgtcaat cacctgaata aaacagactg cgtcgatggt aacgttggcg ttatctttcg
    11881 agataacttc ctgggaaggg atatcgagca cttgctccat catattgatc ttgcgaccaa
    11941 tgcgatccat aaacggcacc accagactga gccccggctg taacgttttg gtatagcgac
    12001 caaaacgttc taccgtccat tgatagccct gcggcacgat tttgacgccc gcgccgacaa
    12061 tgaccagcgc gacaaaaatg agaatcggga taaagataag catcggaaaa acctcctgtt
    12121 gtaccgtcca taatcagcaa aattgctgct tgattaaaca aattatacct gattactgaa
    12181 agagagttcc cccttattcc tgcgaaggat aaactgtttt tagtaaaaat cagaaaaagg
    12241 gaacagcgat gcaggaaaat agtcctttgc ttcagctaca aaacgtagga tatctggcgg
    12301 gtgatgcgaa gattcttaat aacatcaatt tttcgctgcg tgctggcgaa tttaagttaa
    12361 ttaccggtcc ttctggttgt ggcaaaagta cgctgctaaa aatagttgct tcattgatca
    12421 gcccaaccag cggaacgtta ctgtttgaag gtgaggatgt cagcacacta aagccagaaa
    12481 tctaccgcca acaagtctct tactgcgccc agacaccgac gctgtttggc gatacggtat
    12541 acgataatct gatctttccc tggcagatcc gtaaccggca gcctgaccca gccatttttc
    12601 tcgattttct cgaacgcttc gccttgccgg acagcatttt gacgaaaaat atcgccgagc
    12661 tatctggtgg tgaaaaacaa cgcatctcac tgattcgtaa cctgcaattt atgccgaagg
    12721 ttttattgct ggatgaaatc accagtgcgc tggatgaaag taataaacat aacgttaatg
    12781 agatgatcca tcgttatgtg cgcgagcaaa atattgccgt gctgtgggtg acacacgata
    12841 aagacgaaat taatcatgcg gataaagtga ttacactgca accgcatgcc ggagaaatgc
    12901 aggaagcacg ctatgaactc gcataatatt actaacgaat cattagcact ggcattaatg
    12961 ctggtggtgg tggcaatctt aattagccat aaagaaaaac tggcgctgga gaaagatatt
    13021 ctctggagcg tcggacgagc gataattcag ctgattattg tcggctatgt gctgaagtat
    13081 attttcagcg tggatgatgc cagcctgaca ttattgatgg tgttatttat ctgctttaat
    13141 gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta aagcttttat ctcatcgttt
    13201 attgccatta cggtcggggc gggaattacc ctggcggtgc tgattctctc agggtcgatt
    13261 gaatttatcc cgatgcaggt gatccctatc gccgggatga ttgccggtaa cgccatggta
    13321 gcggtggggt tgtgttacaa caatttaggg caacgggtca ttagcgaaca gcaacagatt
    13381 caggagaaac tgagtcttgg tgcgacgccg aagcaggctt cagcgatatt gattcgcgac
    13441 agtattcgcg cggctttaat tccgacggtc gattcagcaa aaacggttgg cttagtgagt
    13501 ttaccaggaa tgatgtccgg gctgatattt gccgggattg atccggtgaa ggcgattaaa
    13561 tatcagatta tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac cattattgcc
    13621 tgctatttaa cctatcgtaa gttttataat tcgcgccacc agttggtggt gacgcaattg
    13681 aagaagaaat gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg gttcatgtgc
    13741 cgatcaacgg aatatcaata caacaatgca tacagctggc ggcgatactt cgacgccagt
    13801 gcatcacccg tacccagcgc agcgaggatc tcctggaacg ttttacgcgt ctgaccgtct
    13861 gcggcggtga gatctttacg cagatgcccg aacagcaact ccagcgcctc ttcattgcgc
    13921 ccaacctgat gcagttgcag cgccagttgc gtcgccagtg cggcatcttc tggattctcc
    13981 gccacctgct gttgcaactg ttgaatttcc ggcgtatcag ccgcctgctt cagcagttcg
    14041 atttgcgcca ccatcccctg gtagcgggtg tcctgatcct gtaacggaat ggttttcagc
    14101 accgcttctg catcttcaga acggttcagc gcaatcagcg tttctgccaa cagcaggccg
    14161 atctccccgt tctgattcga caactgccag gcgtctttca gcaatggcag ggcatcggtg
    14221 aaattgcctt cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc ttcgcgcggc
    14281 agcactttat ccagcagggc gcggatcgcc tcttccggtt gcggcccctg gaagccatct
    14341 accggttgcc cgttctggaa cagatacacc gtcggaatcg cgcgcagacc aaactgggcg
    14401 gcaatcatct gctccgcatc gcagtccagc ttcgccagaa taaattgccc gttgtactgc
    14461 gccgcaaggc tttccagaat tggggttaac tgcaaacagt gctggctacg ttcagaccaa
    14521 aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag gttagattcg
    14581 ttaatgttga caatattttc tacggacatg gagtcgctct ctgttgtcga tattttcttt
    14641 gacatggggg cttaagcgcg cgcttcaact cacccctgca atattttgtc catcacgcgc
    14701 cccggcagca ggcgcttaag caccattact gcccaagtca ccagcgttac cggatagcgc
    14761 atcttcggct tctcgctaat aaaagcatgg cgtactttgt ccaccaccgc ttccggtccc
    14821 aacgtaaagc gggcggcgat gccgggattt tcgactggtt tatcactttg cgtctggttg
    14881 acgttgtcgg tgaagcgagt acgaatggga ccgggttcga tcaggctgac tttaattccg
    14941 ctgtggcgta gctccatgcg cagcgcgtct gaccatgcct ccagcgcata tttactggcc
    15001 gcgtaagcgc cacgacccgg tgtggagatt aatcccatca ccgatgatgt catcacaata
    15061 cgcccttcac cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg gtgtgcgccg
    15121 aaaaagttgg cggaaaactg ctgttccatc tgcgcgcggc tgatggtgga aagggggcca
    15181 tacatgccga atccggcatt gttaaagatc ccatacagac aattatcggt cagggcgatc
    15241 acttcgtcgg ctgcgcgatc aacactttct ggcgaatcca gatcgatcaa cacgccggta
    15301 aatcccatgc tgttcatacg ctcaacatca tccggtttcc ggcaacctgc cagcacatga
    15361 aaaccctggc gttttaattc gagcgcgctt tccaggccaa ttccactgga acatccggta
    15421 attaagaccg atttttgcat aactttacct gtcaggatct ccgttgcttt atgagtcatg
    15481 atttactaaa ggctgcaact gcttcgccat ccagtcggca ataaacggct gggcgtcgcg
    15541 gttgggatga ataccgtcat cctgcatcca ttgtggcttg aggtagacct cttccataaa
    15601 aaagggcagc agcggaacat caaactcttt ggcgagtttg gggtaaatgg cgctaaaggc
    15661 ttcattataa cggcgaccat agtttgcagg cagacgtatt tgcattaaca atggttcagc
    15721 gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt gctgtggctg
    15781 aaaaccacgc aaaccgtcat tgccgcccag ttcaaccagc acccaacgcg gctgatgctg
    15841 tttcagcaga gccggaaggc gcgccagtcc ttgttgcgag gtgtcgccgc tgatgctggc
    15901 attaactacc gacgttttac tctgccactt atcattcaac aaggcaggcc aggccgcgct
    15961 ggcagacatt cgatacccgg cgctcaggct atcacccaga atcaataacg tgtccgctgc
    16021 ggcggcacgg aaggttaaca gggccaggaa caggaagggc aaatgccagc ggaaaacatt
    16081 gttgaagttc atcatcttaa gaagtccgtc ggtcaggggg agcatgaact ctccatcctc
    16141 accggagttg agctggttgt caaacgtggc gagaccatcg cactggtggg cgagtcggga
    16201 tcgggtaagt caaccttgct ggcgatcctc gccgggcttg atgacggcag cagtggcgaa
    16261 gtgagtctgg tgggacaacc gctacataat atggacgaag aagcgcgggc aaagttgcgc
    16321 gcgaagcacg tcggctttgt ttttcagtca tttatgttaa ttcctaccct taacgcgctg
    16381 gaaaacgtcg agcttccggc tctgctgcgc ggtgagagta gcgcggaaag tcgtaacggg
    16441 gcgaaagcgt tgctcgaaca gttagggctg ggtaaacgtc tggatcatct tccggcacag
    16501 ctttccggcg gtgaacagca acgagtggcg ctggcacgag cctttaatgg tcgacctgat
    16561 gtgctgtttg ccgacgaacc caccggcaac cttgaccgcc agacgggcga taaaattgcc
    16621 gacctgctgt tttccctcaa ccgtgaacat ggcaccacgt tgattatggt gacccacgac
    16681 ctgcaactgg cggcacgctg cgaccgctgc ttacggctgg tgaacgggca gttgcaggag
    16741 gaagcatgat tgcacgttgg ttctggcgcg aatggcgttc gccgtcgcta ttaattgtct
    16801 ggctggcgct aagcctggcg gtggcctgcg tgctggcgct gggcaatatc agcgatcgca
    16861 tggagaaggg cttaagccag caaagccgtg agtttatggc gggcgatcgg gcgttgcgca
    16921 gttcacgcga agtgccgcaa gcgtggctgg aggaagcgca aaagcgcggc ctgaaagtcg
    16981 gcaagcagct gactttcgcc acaatgacct ttgcaggcga cacaccgcag ctggcgaacg
    17041 tcaaagcggt ggatgatatc tacccgatgt atggcgatct gcaaactaat ccccctggcc
    17101 tgaaaccgca ggcgggcagc gtattgctgg ccccacgcct gatggcactg cttaacctga
    17161 aaacgggcga caccattgac gtgggcgatg ccaccttgcg gattgccgga gaagtgattc
    17221 aggaaccgga ttccggtttt aaccccttcc agatggctcc gcgtctgatg atgaatctgg
    17281 cggatgtcga taaaaccgga gccgtgcagc cggggagtcg ggtcacctgg cgttataaat
    17341 tcggcggcaa cgagaaccag ctcgacggct atgataaatg gttgttacct cagcttaaac
    17401 ccgaacaacg ctggtacggt ctggaacagg acgaaggcgc gctggggcga tcgatggaac
    17461 gctcgcaaca gttcctgctg ctttcggcgc ttctgacctt gctgctggca gtggcagcgg
    17521 tggcggtagc gatgaatcat tactgtcgca gtcgctacga tctggtggcg atcctcaaaa
    17581 cgctgggggc agggcgagcg caactgcgta agctaatcgt cggtcagtgg ttgatggtgc
    17641 tgacgctttc agccgttacc ggtggggcca taggcctgtt gttcgaaaac gtgttgatgg
    17701 tgctgctcaa gcccgttctg cctgctgcac taccgccagc cagcctctgg ccgtggctgt
    17761 gggcgcttgg caccatgacg gtcatctcgc tgctggtggg gctacgacca taccgcttgt
    17821 tgctggcaac gcagccttta cgcgtattac gtaatgatgt ggtagcgaac gtctggccgc
    17881 tgaagtttta tctgccgatt gtcagtgtgg tggttgtgct gctgctcgcc ggattaatgg
    17941 gtggcagcat gctgctttgg gcggtgctgg cgggcgcggt agtactggct ttgctgtgcg
    18001 gtgtgctggg ctggatgcag ctgaatgtac ttcgccgcat gacgctgaaa tcgctacctc
    18061 tgcgcctggc ggttagccgc ctgttacgtc agccgtggtc aacgttaagc cagctttcgg
    18121 cattttcgct ctcctttatg ctgctggcgc tgttgctggt attgcgtggc gatctgctcg
    18181 accgctggca gcagcagcta ccgccagaaa gcccgaacta ctttttaatt aacatcgcca
    18241 cagaacaggt agcaccgcta aaagcgttcc tcgcggaaca tcatatagtc ccggaatcat
    18301 tttatccggt agtgcgggcg cggctgacgg cgattaacga taagccgaca gaaggtaatg
    18361 aagatgaggc gcttaatcgc gaactcaatc ttacctggca aaatacgcgg cccgatcata
    18421 atccgattgt cgccggtaac tggccgccaa aagccgatga agtgtcgatg gaagaggggc
    18481 tggcgaaacg cttaaacgtt gccctcggtg ataccgtgac ttttatgggc gatacccagg
    18541 agttccgcgc taaagtgacc agcctgcgca aagtggactg ggaaagtctg cggcctaatt
    18601 tctattttat tttcccggaa ggggcattag acgggcaacc gcagagctgg cttaccagtt
    18661 tccgctggga gaatggcaac ggcatgttga cgcaactcaa ccgccagttc ccgaccatta
    18721 gcctgttaga tattggcgcg attttaaaac aggtcggtca ggtgctggag caggtaagtc
    18781 gggcgctgga ggtgatggtg gtgctggtca ccgcctgcgg tatgttgctg ttgctggcac
    18841 aggtgcaggt gggaatgcgt cagcgtcatc aggagctggt ggtgtggcgc acactcggtg
    18901 cggggaaaaa actgctgcgt accacgttgt ggtgtgagtt cgccatgctt gggtttgttt
    18961 ctggcctggt agccgcaatt ggtgcggaaa cggcgctggc agtgttgcag gcgaaagtgt
    19021 ttgatttccc gtgggagcca gactggcgat tgtggattgt tctgccgtgc agcggtgcac
    19081 tgctgctgtc gcttttcggc ggctggctgg gtgcgcgact ggttaagggt aaggcgctgt
    19141 tcaggcagtt tgcggggtga tgacaggtgt tagattgcct gatgcgctac gcttatcagg
    19201 cctacgaaga gcgtgcaata tattgatttt gcaagattgt gtaggccgga taaggcgttt
    19261 acgccgcatc tggcatgaac agtgtgcatt ttggcaataa atatgaaacc aactgtcatg
    19321 tcggtttttt atatctgtat tctggtgcaa ccattctgtt ctcacgacaa tcgccgtttc
    19381 agaataccgc ccatcactgc gtcttaaccc attccgccac ttccgcccac tcaccgcgaa
    19441 agacaacttt ttccgctttt ttctcaagct gatagcgata catcgggtcg taatattctt
    19501 taagtaacgg caccagccag gccagatgac cgtcggtgct gtcggtggtg agttgcgttg
    19561 tcagcgccgt atccagctgg gcagcgagtt cgttatagcg ctgtaacccc agccgacgct
    19621 taatagccga aagtccgtga tgcaggtatt cgcaatattc ctgccagccc tgttcgtcgc
    19681 cgtacgcgtg ggtaaaatca tgatgcatac gcaagaaata ctcttcgttc aggcgctcaa
    19741 gacggatctc aaacggatct tctaccaccg caatcgccgc ctgagtcatt cgctcgcgca
    19801 ggcattccgg caggtgattc gaaccgatca tccggctttc gtcttccagc acccacaggc
    19861 gcaaatcctg acgggcgtcg gtttttagca tttcggcagc cagcaggttt tcaaaactcg
    19921 cctggctaag ttgtggttgt aaggtgcgac caaacgccga accgcgatga cgcgctaacc
    19981 cttccagatc aacaccgttc ggctgttgct gcactaacag cgttttaccg ctgccggtac
    20041 aaccgccaat cagcactatc ggtttttgtg ccagttcaat agtcgcctga atcgcggctt
    20101 ggcgcagtgc cttataaccg ccttccacca gcggataatc aatccccgct gcatacaacc
    20161 agctttgcac aatatgtgag cgctgaccgc cacgggcgca gcagagaata ccttgcggat
    20221 tttgcaggca cgctgcccgc caggcgtcca tgcgctgctg acgaatttca cccgccacca
    20281 gtttatgccc cagcgccagc gccgcgtctg agccttgctg tttataacaa gtgccaacgg
    20341 cggcgcgttc atcgttattc attaacggca ggttgatagc ggcgggcatt gcgccttgct
    20401 caaactcgat aggggcgcga acatcaataa tgggcgtatc agcaatcagc agggcacgat
    20461 agtcctgttc cgtgtgtctc tcttgcatag ttaaaagtga acctcaaatc agcttgcgcg
    20521 ctattttacg cgccaacgcg caaggaaact tgatttttaa ctgcgtgggt tgccgaaaat
    20581 ttctaaaaat ccgctgattt ccggcctgcg ctgggtaaac aaggtcacaa tatcttctac
    20641 cgctttgccg ctgccaaatt tgcgccatgc cagactcaat ggcgaaggag ggcgcatcgt
    20701 tgggattacc cggctgacca gttgttgatt atcgatcatt gactggcaaa gtgattttgg
    20761 caaaaaacca atgccaacac ccgccagatg ggcggcgatt ttcgtttcca tatcgggaac
    20821 aataatctct ttttgccccg gcaatcgcca ggcgacgcgt ttggttaagg ttcgggcgct
    20881 gtcttcaata ttgaccgccg gaaagcgccg caactgcgct tctgttagcg gctcttcaac
    20941 gttcgccagc ggatgatccg ccgccatgac aaagcgccat tgcaccgatc ctaagggatc
    21001 aagactaaag gtatttgcca gcgcctcagt tcccgtgacc ccgatagcca gcgaaaaacc
    21061 ttcgtacaat agcgagtccc agacgcccat atagatttgt cgggagatgt gaaactgggt
    21121 aaaggggtaa cgctcattca gccacgccag caactgggcg acggcctggg ggttatagag
    21181 caggttgttg atgacaatat tcacctagcg ttccacgcca tcattcacct gttgcagctc
    21241 gctgggcata ctttccagcc agctcagcca gtctctggcc tgggaaagta gatgctcgcc
    21301 agccgctgtc aacgtcacgc tgcgagtcgt gcggaaaaac agcgccactc cggtattctc
    21361 ttccagaagt ttaatgcgat aactgatcgt cgccgtggtt ttacataatc gttctgccgc
    21421 ttttgaaaaa cttcctgttt cagcaaccgc aatgaaagtc cgcaaggttt ctggatcgaa
    21481 catcttcagg tatccccttt taaatccgca agttgcgtga ttttcttatc ctctgattta
    21541 tcagtatttt tacatgataa ccctgttcaa tttgtggact aaatctagtt ttggaaaaat
    21601 attccaactt ttgtattgat gttgttctct taaggtttta gat
//
LOCUS       DAJVQG010000066        21455 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJVQG010000066 DAJVQG010000000
VERSION     DAJVQG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.066"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(331..876)
                     /gene="hemG"
                     /locus_tag="OQM83_002178"
     CDS             complement(331..876)
                     /gene="hemG"
                     /locus_tag="OQM83_002178"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCS1520440.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(888..2339)
                     /gene="trkH"
                     /locus_tag="OQM83_002179"
     CDS             complement(888..2339)
                     /gene="trkH"
                     /locus_tag="OQM83_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCS1520441.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2378..2992)
                     /locus_tag="OQM83_002180"
     CDS             complement(2378..2992)
                     /locus_tag="OQM83_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCS1520442.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITSVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2992..4323)
                     /gene="pepQ"
                     /locus_tag="OQM83_002181"
     CDS             complement(2992..4323)
                     /gene="pepQ"
                     /locus_tag="OQM83_002181"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCS1520443.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGQQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4513..6702
                     /gene="fadB"
                     /locus_tag="OQM83_002182"
     CDS             4513..6702
                     /gene="fadB"
                     /locus_tag="OQM83_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCS1520444.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6712..7875
                     /gene="fadA"
                     /locus_tag="OQM83_002183"
     CDS             6712..7875
                     /gene="fadA"
                     /locus_tag="OQM83_002183"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCS1520445.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQAGLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(8065..8766)
                     /gene="fre"
                     /locus_tag="OQM83_002184"
     CDS             complement(8065..8766)
                     /gene="fre"
                     /locus_tag="OQM83_002184"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCS1520446.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8813..10306)
                     /gene="ubiD"
                     /locus_tag="OQM83_002185"
     CDS             complement(8813..10306)
                     /gene="ubiD"
                     /locus_tag="OQM83_002185"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCS1520447.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10473..10961
                     /gene="rfaH"
                     /locus_tag="OQM83_002186"
     CDS             10473..10961
                     /gene="rfaH"
                     /locus_tag="OQM83_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCS1520448.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10958..11740)
                     /gene="tatD"
                     /locus_tag="OQM83_002187"
     CDS             complement(10958..11740)
                     /gene="tatD"
                     /locus_tag="OQM83_002187"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCS1520449.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(11782..12558)
                     /gene="tatC"
                     /locus_tag="OQM83_002188"
     CDS             complement(11782..12558)
                     /gene="tatC"
                     /locus_tag="OQM83_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCS1520450.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12561..13076)
                     /gene="tatB"
                     /locus_tag="OQM83_002189"
     CDS             complement(12561..13076)
                     /gene="tatB"
                     /locus_tag="OQM83_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCS1520451.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(13080..13349)
                     /gene="tatA"
                     /locus_tag="OQM83_002190"
     CDS             complement(13080..13349)
                     /gene="tatA"
                     /locus_tag="OQM83_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCS1520452.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13428..15068)
                     /gene="ubiB"
                     /locus_tag="OQM83_002191"
     CDS             complement(13428..15068)
                     /gene="ubiB"
                     /locus_tag="OQM83_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCS1520453.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(15065..15670)
                     /gene="ubiJ"
                     /locus_tag="OQM83_002192"
     CDS             complement(15065..15670)
                     /gene="ubiJ"
                     /locus_tag="OQM83_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCS1520454.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15684..16439)
                     /gene="ubiE"
                     /locus_tag="OQM83_002193"
     CDS             complement(15684..16439)
                     /gene="ubiE"
                     /locus_tag="OQM83_002193"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCS1520455.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16534..17961)
                     /gene="rmuC"
                     /locus_tag="OQM83_002194"
     CDS             complement(16534..17961)
                     /gene="rmuC"
                     /locus_tag="OQM83_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCS1520456.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(18102..18863)
                     /gene="udp"
                     /locus_tag="OQM83_002195"
     CDS             complement(18102..18863)
                     /gene="udp"
                     /locus_tag="OQM83_002195"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCS1520457.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            19125..19937
                     /locus_tag="OQM83_002196"
     CDS             19125..19937
                     /locus_tag="OQM83_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS1520458.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            20331..21392
                     /gene="hcp"
                     /locus_tag="OQM83_002197"
                     /pseudo
     CDS             20331..21392
                     /gene="hcp"
                     /locus_tag="OQM83_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
BASE COUNT         5262 a         5740 c         5378 g         5075 t
ORIGIN      
        1 cactcttgag acttggtatt catttttcgt cttgcgacgt taagaatccg tatcttcgag
       61 tgcccacaca gattgtctga taaattgtta aagagcagtg ccgcttcgct ttttctcagc
      121 ggcgcggggt gtgcataata cgctttcccg ctacagagtc aagcatttat ttttgctttc
      181 tctgtcggaa ttctcaggag aaccccgctg acccggcggc gtgtttgccg ttgttccgtg
      241 tcagtggtgg cgcattatag ggagttattc cggcctgaca agaggaaatt taaaataatt
      301 ttctgaccgc gcatttttta ttctttatgc ttatttcagc gtcggtttgt tggttaactg
      361 ggcgatttct cgggcgaaat tcgccacctg ctcccaatcg gtatagacaa cttctttgcg
      421 cgtatccgtt tcaccgcctg acatcttcat aattagcttg atcataaaac gatcgtacca
      481 gcgataacgt gggtaacgca gcgccccggc aatgaccgcg cagcgatcgg gacgccattg
      541 cgagttcatc agaaacttcc gcgcgtagct gttggtctgt ggagtacgct tctccggttt
      601 gcgcgccacc aggtttacgg aataaaaggc gctcggcatc gaattcagtc gcgtcgcatg
      661 ttttttgaca aattcctgga acgcagagtg gtagtgacca tagcgaatag aagcaccaat
      721 gaccacacgg tcataatttt cccattgtgg ttcttcaatg cggtgcacat tggcgacatc
      781 cgtctggatc cccagttctt tcagttccga agccaggtag gaggcaatct cgcgcgtttg
      841 tccgtccctt gttgagaaaa gaattaatgt tttcacgtat tactccatca ttcacgccag
      901 aaagtcgggg taaagagcac cagcaatgta aagacctcga gacgaccaaa cagcatgttg
      961 gcaatcagga tccatttagc caccgggttc atactggtaa agttatcagc aaccacgcca
     1021 agccctggcc ccaggttatt caatgtcgca acaactgaag caaaggcaga aaagtcatcc
     1081 acgcccgtgg cgataatcgc cagcatactg acaataaaca ccaatgcata ggcggagaaa
     1141 aatccccaaa cggcttcgag gatacgttcc ggcagtgcgc gattccccag cttaatgcta
     1201 tagacggcgt tcggatgcac cagtcgtttc agctcacggt tcccctgctt aaacagcagc
     1261 aggatgcgga tcactttcag gccaccgccc gttgacccgg cgcaaccgcc gataaaagct
     1321 gaacataaaa gcagtaccgg caaaaagagc ggccagcggg caatgctgtc agtcgtaaac
     1381 ccggcggttg tcgccatcga taccacctgg aaaaacgcct ggttaattgt catcagcgcc
     1441 gaactgtaga cattatgaaa ccacagtacg agtgtacaaa taaccaccag cgtaaactgt
     1501 acgccgataa acatgcgaaa ttccggatcg cgccaataaa ccttcagact acgcccactt
     1561 aacagtgaaa agtgcagacc gtagttacag ccggagatca gcaggaagat agcaatgata
     1621 gtgttaatag tcgggctgtc gaaataaccg atactggcat catgtgtcga gaagccgcca
     1681 atagcgatag tcgcaaagct atggccgatg gcatcaaagg catccattcc ggcaaaccac
     1741 aacgccagcg cacaggcgac ggtcagcaag acataaatca accacagggt tttcgccgtt
     1801 tccgcaattc gcgggcgcat tttgttatct ttcagcgggc cgggcatttc tgcgcgatag
     1861 agctgcatcc cacccacgcc gaggataggc agtatcgcaa ccgctaacac gatgatcccc
     1921 atcccgccaa accattgcag catctggcga taaaagagga tggcatgagg gagcgaatcc
     1981 agccccacca gcgtagtggc cccggtggtg gtcaggccag agaaagattc aaaaaacgca
     2041 tcggtaatcg tgaggttcgg gctttccgag aagataaaag ggagcgcacc gacactaccc
     2101 agcacggtcc agaacagcac cactatcaga aacccctcac gggatttcag ttcgcccttc
     2161 tctttacggt tcggccacca cagcatagaa ccaatggcga gggcgacaaa aaaggtctgg
     2221 gtaaaagcgc ggcccgctcc atcccggtag atgagtgcta ccagcccagg gataatcatg
     2281 gtccctgaaa ataagatgac cagtagtcca acgattcggg taatggcgcg aaaatgcatc
     2341 tctgccgctt ccttagttct gcaaaacgaa gtggggatta ttcttcaatc gctaacaatt
     2401 gcaatgaacc acggctaaaa tccgccagct ttgcggaaaa ttcagccact ttcgccgccg
     2461 gaagcgccac ccgcagcaga acgaatgcct gataatcact gttgataatt ttgccgtcac
     2521 actgccccag caacgcttca atgccggtta actgatgata ttcacattgc aaagtatatt
     2581 cggttaatgg cgtcttgcgt tgggtcgtta gctggcgcag cgcctgattc acgccgccgc
     2641 catacgcttt cactaaccca ccggtgccaa gcaatatgcc gccgtagtag cgcactacca
     2701 cagaggtaat ttccccgacg ccgctgccca ttagctgggc gagcatcggt ttacctgccg
     2761 ttcctgccgg ctccccgtcg tcagagaaac ccagctgttg agaatcatcc ggcgcacccg
     2821 ccacccacgc cacgcaatgg tggcgggcat cggggtgttc tgcccgcacc gattcaacaa
     2881 acgctttcgc cgcctcaacg ccatcggtat gcgccaacat cgttatgaag cggctttttt
     2941 tgatctcttc aacgaccgtg accggtgccg caggaattaa ccagctttcc atcacgccag
     3001 tttcagatcc cgggtcatgt tttccacgtt gttttcgtgg atcaccacgt tgtcttcgat
     3061 acgaatgccg ccgaacggtt tcagtgcttc aattttctgc cagttgaagt gcttgctgaa
     3121 ctgcccttca cgccacggtg ccagtagcga ttcaatgaag tagatacccg gttcgatggt
     3181 taacaccata cccggctgga gaatacgggt gcagcgcagg tacggatatt ttgccggtgc
     3241 cgcgaggtgt gtaccgctat catcctgcat aaaaccggct acgtcatgca cctgctggcc
     3301 cagcggatga ccgataccat gcggcataaa cggtccggtg agatcgtttt cgaccatcgc
     3361 ttcttcactc atatcggtga tgatttgatg tttacgcagc agtttggcga tgcgctgatg
     3421 gaactggatg tggtaatcca catagctaac gcctgctttc atggtggcga tcagcgccag
     3481 ttgttcatca tttacgtctt tcaccagctg tgcgtagtcg ttgtcgcttt ttgccgacca
     3541 ggtacgagtc aggtcagccg catagccgtt atattcggcc ccggcatcca gcaggaagct
     3601 gcgcatctct tccggtgcct gatgatccag tttggtgtaa tgcagcaccg aagcgtgttc
     3661 gttaagcgcc acaatgttgc tgtaaggtac gtcggtatca cgatgaccgg tcgcggtcag
     3721 ataggcgatg ttgatatcga actcgctcat gccagaacgg aacgcttctt ctgccgcacg
     3781 atgaccgttg accgccattt tctgcgcttc acgcatacag gccagttcgt actcggtttt
     3841 gaaggagcgg tagtaatgca ggtagtcgat cacccctttc gggttgatgt tgctggcctc
     3901 aatacccagt tgcagcgcac gttccggcac cggaccgata taaccgatat tgccgcgcgc
     3961 agcgggcagc aggctaccaa tgccatcggc tttcggcagt gcgatcactt ccacatcttc
     4021 agtccagaag gaggtcggca gcggttcgac gttgtgccag taatcaaccg gcagatagaa
     4081 ccacagtttc ggcttgttca cgccatccac cagcaaccag cagtttggca cctgagttac
     4141 cggcacccac gctttgaatt gcgggttcac tttaaacgga tagggatgat cgtcgagaaa
     4201 aacgttgaac agctcgccgg agtgaataag taatgcatcc agcttgaagc gcgccagcgc
     4261 atcgcgagtc cgttcttgta aggtagctat atgattttta tagagcgagg ccagtgattc
     4321 cattttttac ccttctgttt ttttgacctt aagtctccgc atcttagcac atcgttcatc
     4381 cagagcgtga tttctgccga gcgtgatcag atcggcattt ctttaatctt ttatttgcat
     4441 atttttaaca caaaatacac acttcgatgc atctggtacg accagatcac cttgcggatt
     4501 caggagactg acatgcttta caaaggcgac accctgtacc ttgactggct ggaagatggc
     4561 attgccgaac tggtatttga tgccccaggt tcagttaata aactcgacac tgcgaccgtc
     4621 gccagcctcg gcgaggccat cggcgtgctg gaacagcaat cagatctaaa agggctgctg
     4681 ctgcgttcga acaaagcagc ctttatcgtc ggtgctgata tcaccgaatt tttgtccctg
     4741 ttcctcgttc ctgaagaaca gttaagtcag tggctgcact ttgccaatag cgtgtttaat
     4801 cgcctggaag atctgccggt gccgaccatt gctgccgtca acggctatgc gctgggtggt
     4861 ggctgcgaat gcgtgctggc gaccgattat cgtctggcga cgccggatct gcgcatcggt
     4921 ctgccggaaa ccaaactggg catcatgccg ggctttggcg gttctgtacg tatgccacgt
     4981 atgctgggcg ctgacagtgc gctggaaatc attgccgccg gtaaagatgt cggcgcggat
     5041 caggcgctga aaatcggtct ggtggatggg gtagtcaaag cagaaaaact ggttgaaggc
     5101 gcaaaggcgg ttttacgcca ggccattaac ggcgacctcg actggaaagc gaaacgccag
     5161 ccgaagctgg aaccgctaaa actgagcaag attgaagcca ccatgagctt caccatcgct
     5221 aaaggaatgg tcgcgcaaac ggcggggaaa cattatccgg cccccatcac cgcagtaaaa
     5281 accattgaag ctgcggcccg ttttggtcgt gaagaagcct taaatctgga aaacaaaagt
     5341 tttgtcccgc tggcgcatac caacgaagct cgcgcactgg tcggcatttt ccttaacgat
     5401 caatatgtaa aaggcaaagc gaagaaactc accaaagacg ttgaaacccc gaaacaggct
     5461 gctgtgctgg gcgcaggcat catgggtggc ggtatcgctt accagtccgc gtggaaaggc
     5521 gtgccggttg tcatgaaaga tatcaacgac aagtcgttaa ccctcggcat gaccgaagcc
     5581 gcgaaactgc tgaacaagca gcttgagcgc ggcaagatcg atggtctgaa actggctggc
     5641 gtgatctcca cgatccaccc aacgctcgac tacgccggat ttgaccgcgt ggatattgtg
     5701 gtagaagcgg ttgttgaaaa cccgaaagtg aaaaaagccg tactggcaga aaccgaacaa
     5761 aaagtacgcc aggataccgt gctggcgtct aacacttcaa ccattcctat cagcgaactg
     5821 gccaacgcgc tggaacgccc ggaaaacttc tgcgggatgc acttctttaa cccggtccac
     5881 cgaatgccgt tggtagaaat tattcgcggc gagaaaagct ccgacgaaac catcgcgaaa
     5941 gttgtcgcct gggcgagcaa gatgggcaag acgccgattg tggttaacga ctgccccggc
     6001 ttctttgtta accgcgtgct gttcccgtat ttcgccggtt tcagccagct gctgcgcgac
     6061 ggcgcggatt tccgcaagat cgacaaagtg atggaaaaac agtttggctg gccgatgggc
     6121 ccggcatatc tgctggacgt tgtgggcatt gataccgcgc atcacgctca ggctgtcatg
     6181 gcagcaggct tcccgcagcg gatgcaaaaa gattaccgcg atgccatcga cgcgctgttt
     6241 gatgccaacc gctttggtca gaagaacggc ctcggtttct ggcgttataa agaagacagc
     6301 aaaggtaagc cgaagaaaga agaagacgcc gccgttgaag acctgctggc agaagtgagc
     6361 cagccgaagc gcgatttcag cgaagaagag attatcgccc gcatgatgat cccgatggtc
     6421 aacgaagtgg tgcgctgtct ggaagagggc attatcgcca ctccggcgga agcggatatg
     6481 gcgctggtct acggcctggg cttccctccg ttccacggcg gcgcgttccg ctggctggac
     6541 accctcggta gcgcaaaata cctcgatatg gcacagcaat atcagcacct cggcccgctg
     6601 tatgaagtgc cggaaggtct gcgtaataaa gcgcgtcata acgaaccgta ctatcctccg
     6661 gttgagccag cccgtccggt tggcgacctg aaaacggctt aaggagtcac aatggaacag
     6721 gttgtcattg tcgatgcaat tcgcaccccg atgggccgtt cgaagggcgg tgcttttcgt
     6781 aacgtgcgtg cagaagatct ctccgctcat ttaatgcgta gcctgctggc gcgtaacccg
     6841 gcgctggaag cggcggccct cgacgatatt tactggggtt gtgtgcagca gacgctggag
     6901 cagggtttta atatcgcccg taacgcggcg ctgctggcag aagtgccgca ctctgtcccg
     6961 gcggttaccg tcaatcgctt gtgtggttca tccatgcagg cactgcatga cgcagcacga
     7021 atgatcatga ctggcgatgc gcaggcaggt ctggttggcg gcgtggagca tatgggccat
     7081 gtgccgatga gtcacggcgt cgattttcac cccggcctga gccgcaatgt cgccaaagcg
     7141 gcgggcatga tgggcttaac ggcagaaatg ctggcgcgta tgcacggtat cagccgtgaa
     7201 atgcaggatg cctttgccgc gcggtcacac gcccgcgcct gggccgccac gcagtcgggc
     7261 gcatttaaaa atgaaatcat cccgaccggt ggtcacgatg ccgacggcgt cctgaagcag
     7321 tttaattacg acgaagtgat tcgcccggaa accaccgtgg aagccctcgc cacgctgcgt
     7381 ccggcgtttg atccagtaag cggtacggta acggcgggta catcttctgc actttctgat
     7441 ggtgccgcgg ccatgctggt gatgagtgaa agccgcgccc gtgaattagg tcttaagccg
     7501 cgcgcccgtg tgcgttcgat ggcggtcgtt ggttgtgatc cgtcgattat gggttacggc
     7561 ccggttcctg cctcgaagct ggcgctgaaa aaagcggggc tttctgccag cgatatcggc
     7621 gtgtttgaga tgaacgaagc ctttgccgcg cagatcctgc cgtgcattaa agatctcggg
     7681 ctaatggagc agattgacga gaagatcaac ctcaatggtg gtgcgatcgc gctgggtcat
     7741 ccgctgggtt gttccggtgc gcgtatcagc accacgttgc tgaatctgat ggaacacaaa
     7801 gacgttcagt ttggtctggc gacgatgtgt atcggtctgg gtcagggtat tgcgacggtg
     7861 tttgagcggg tttaaccact tgccggatgc ggcgtgaacg ccttatccgg tctaccgatc
     7921 ctgcaccaat gtaggcctga taagacgcgc cagcgtcgca tcaggcatcg tgcaccgatt
     7981 gccggatgcg acatagatct aaatgccact gaataaagtt ttagttgccg ttcttcccgc
     8041 ctgtcagggg cgggttttta tatctcagat aaatgcaaac gcatcgccaa acagacgatc
     8101 ttcccgcgca ttacgctcac tgcaaaacag gtcacgagca attttcgcca tctcaaaacg
     8161 tccggcaata tagatatcat gctctgccag cgtaccatga tcctgcaaga ccgccgttaa
     8221 cacggtgcca gtacgcccac gccagcccgc ttccggttgt tcaaccaccg gcaccacttg
     8281 cagaccagga tgcttcagcg aaagcgcctc aagctcgcag agatcataca gatgttgctc
     8341 ttcacgccca ccccagtaaa tggtgatatc gcgattggga ttacgcgcca acgccgtcaa
     8401 taaaatcgag cgcgcatagg agaacccggt gccgcccgca atcaaaatca tcggacgctc
     8461 ttcatcatcg cgcagccacg cttctccgtg gggaatatcg accacgattt gatgatcttt
     8521 gaggatgcgg tccatgactg ctttcgcgta aaggttgatt tcagaagcgc caatatgcag
     8581 ctcgataaac cctttttcat ccggcgtcga agccattgaa aacggacgtt tgtcgcgctc
     8641 atccatcact accatcaaat actgaccagc acgaaaagaa aaggccgcgt ctggtacgat
     8701 tcggacacgg tatacagtat ccgtgatagc ttctaccgag gtcactttac agcttaaggt
     8761 tgtcatgcgc tttctctgtc ggatcaaata aatagggcaa aactaacgcg catcaggcgc
     8821 ttttaccgtt gttaaaaata gccagttcat cccagatggc gtcaatatgc gcgacgacat
     8881 caggatcttt tttgatggga cgtccccatt cacgctgggt ttcccccggc catttattcg
     8941 tggcatccag ccccattttt gaacccagcc cggagacagg cgaggcaaaa tccagataat
     9001 caataggcgt attttctacc agaacagtat cccgcgccgg gtccatacgg gtggtaatcg
     9061 cccaaatcac atcgttccag tcgcgtgcgt tgacgtcatc atcgcaaacg atcacaaatt
     9121 tagtgtacat aaactggcgt aagaacgacc agacgcccat catgacgcgc ttcgcgtgtc
     9181 cggcgtactg ttttttgatt gtcactaccg ccagacgata agaacagcct tccggtggca
     9241 ggtaaaaatc gacaatttcc gggaactgtt tttgcagaat cggcacaaac acttcgttca
     9301 gtgcgacacc cagcaccgcg ggttcatccg gcggacgacc agtataggtg gagtggtaaa
     9361 tcgcatcttc acgctgggta atatgcgtca cggtaaatac cgggaaacta tcgacttcgt
     9421 tatagtaacc ggtgtggtcg ccatacggcc cttccggcgc agtttcgcct tgttcgatat
     9481 acccttccag cacaatctcc gcactggcgg gcacttcaag gtcattggag atacacttca
     9541 ccacttcggt cttggtaccg cgcagcaatc cggcaaacgc atactctgaa agtgtatccg
     9601 gaacgggggt gactgcaccg agaatcgtgg cgggatcggc acccagcgcc acagaaaccg
     9661 ggaaacgttc gcccggatgc gccgcacacc actcctgata atccagcgcg ccgccgcgat
     9721 gcgacagcca gcgcataatc agtttgtttt taccaatcag ctgctggcga taaatgccca
     9781 gattctgccg ctctttatgc gggccgcgcg ttacggtcag cccccaggta atcagcggcg
     9841 cggcatcttc cggccagcag gtcataatgg gaatgcgatt gagatcgacg tcatcgccag
     9901 agacgatttt ttgttggcag ggcgcaccac gcagccgctt tgtcggcatg ttcaatactt
     9961 gcttaaactg cggcagttta tcaaacaggt cgcggaaacc ttttggcggc tcgggctctt
    10021 tcagaaacgc caataattta ccaacttcac gcagcgccga aacatcttcc tgccccatgc
    10081 ccatcgccac gcgctttggc gtaccgaaca ggttgcacag caccggcatt gagtagcctt
    10141 tagggttttc gaacaacagc gcaggcccac cggcacgcaa agtgcggtca gcaatttcag
    10201 tgatttccag atgcggatcc accgggagcg tgatacgttt tagctcaccc tgctgttcaa
    10261 gcagcgtcaa gaagtcgcgt aaatcgttat atttcatggc gtccattgta gcctcttaat
    10321 ctacgcccat tatacggcgt tcatctttgc gatgctgtaa atttgttaaa ttagcgtgaa
    10381 ctctgacggt ataacgcaaa ccggggaata taattaactt agcgtaaagc ttttgctatc
    10441 cttgcgcccc gattaaacgg ataagagtca ttatgcaatc ttggtattta ctgtactgca
    10501 agcgcgggca acttcaacgt gcccaggaac accttgagag acaggctgtg aattgcctgg
    10561 caccgatgat caccctggaa aaaatcgtgc gtggaaaacg tactgcagtc agtgagccat
    10621 tgtttcccaa ctacctgttt gtcgaatttg atccagaagt gattcatacc acgactatca
    10681 acgcgacccg cggtgtcagc cacttcgtgc gctttggcgc gtcgctagcg atagtcccat
    10741 cggcggtgat tcatcagcta tcggtatata aaccgaaaga cattgtcgat ccggcaaccc
    10801 cttatccggg agataaggtg attattaccg aaggcgcgtt cgaaggcttt caggccattt
    10861 tcaccgaacc cgatggtgag gctcgctcca tgctattgct taatcttatt aataaagaga
    10921 ttaagcacag tgtgaagaac accgagttcc gcaaactcta aaacgcaatc ccaaaccgtg
    10981 ttttgacatt agcatccgtg gtggcagcca gccatgcggc atcttctcca cgccagtgcg
    11041 caatacgttg caaaatatgg ggcagatggg ctggctcgtt gcgccgggat gatggctttg
    11101 gcgtgagatc gcgagggagc agatacggcg catcagtttc gatcagtaat ttttccgccg
    11161 gaatcaacgg caaaagttcc cgcagctcca gtccgcgtcg ttcatcgtaa acccaaccgg
    11221 taatgccgat ataaattcca tgcgccacgc acgcctgcat ctcttcgcgt gtgccggtaa
    11281 agcaatgaag aaccgcacca ggcagtttat ccagccacgg ctccagcaat gtcataaacc
    11341 gctcgtgggc atcgcgacag tgcataaata ccggcatgtt taaatctgcg gcaatgcgta
    11401 gctgggcaac aaaagcgcgt tcctgctctt ccggcgtcga aaagttgcgg ttaaagtcga
    11461 gaccacattc accaatcgcc accacttctg gctgcgcggc cagctcaata atcgcttctt
    11521 cagtcgcagc ttgccactgg ctgctgtcgt gaggatgtac gcccgccgtt gaccaacagg
    11581 acgaatactg acgcgccagc ttttgcgcct gctggctttc acgcaggtta gtgccggtga
    11641 tgagtagccc attaactccc gcgtcaaaag cgcgcgctac aacatcatca cggtctttcg
    11701 caaattgcga actggtcaaa ttaacgccga tatcaaacat cctgtactcc atatgacaac
    11761 cgccctgacg ggcggttgaa tttattcttc agttttttcg ctttctgctt cagcgtcgtt
    11821 ttcctcttcc cgatttcgcc ctttaccaac gtaaaagcgt gagaagaaga caccgatttc
    11881 aaacagacag tacatcggga tcgccaatag cgtttgcgag aagacatccg gcggcgtcag
    11941 caacatcccg acaacgaatg caccaaccag cacatacggg cgttttttgc gtaagtcttc
    12001 tggcgaggta atccccatcc agcacagcag cacaattgcc accggcactt caaaggagac
    12061 accaaacgcc ataaacagcg ccataacgaa gcttaaatag ctggcgatgt cggtggatac
    12121 ctgcacccct tccggcgcgg tattggcaag gaagccaaat gccagcggaa agaccacaaa
    12181 gtaggcgaat gccatgccga tataaaacag cagagagctg gaaaccagca gcggcaccac
    12241 caggcgacgt tcatgcttat acagcgctgg ggcgataaat gcccacacct gatagagaat
    12301 caccggcgct gacagaatca gcgacaccat aaaggtcagc ttgatcggcg taaagaacgg
    12361 cgaggccacg tcggtggcga tcatcgttga accttgcggc aactgcttga tcaatggcgc
    12421 ggataccagg tgatagatgt cattggcgaa atagaccaga cacaggaata tcacgatcac
    12481 cgcgataatg cagttcagca gacgcttacg cagctcaatt agatgcgtga taagcggttg
    12541 agtatcttct acagacatgt ttacggttta tcactcgacg aaggggaagg tgcagcggtt
    12601 ttcggttcag cgtccgcagc aggttttacc accggctctg gcgtggtttc tggcttctgt
    12661 tccggcgaac tggcctgcgt ttgtgcagcg gcaggcgtta cgccctcatg cgcagcttca
    12721 ttatctttca ccaccgggtt atggatggtg tgcgcttcat cgctcgcctt ttcaggatcg
    12781 tttgcaacgt aggaacgctt catcgactcc gcggcctggc gtagttcatc catcgacgct
    12841 ttcagttcgg gcgtcaggtt agtgaggctc gccttttcaa cctttttcag actgtcctga
    12901 aactcctgga gttttaactc ctgggtcagt tcgttctgca ccgttgtcgc cagtgaacgc
    12961 aacgcgcgaa tccagcccgc taccgttttt accgccacag gcagtcgttg cggccccaga
    13021 acgacgaggc cgatgatgaa caccaatagc agttcgctaa aaccgatatc aaacacggat
    13081 tacacctgct ctttatcgtg gcgcttcgcg tcttctgttt tagcctgttc ctgattcgta
    13141 tccgcctgct tatcggcgat agttttcgca gtaaaatcag catcctgact ggttttatcc
    13201 tgctttggtt catcatcgct cattgctttt ttaaagcctt tgatcgacgc accaagatcg
    13261 gaaccgatgg agccgagctt tttggtgcca aaaagcagta caacgatgac ggcaataatc
    13321 aataactgcc aaatactgat accacccata catgttcctc tgtggtagat gatgattaaa
    13381 caaggccgca ttatacgtta cacgacccgc cttgagcgat gaaaaaatca gcgtgttttg
    13441 cgccaaccga caaaccaggc gatcagacca cctgccatta accagccggg catcagcccc
    13501 cattcaggtc ggctgaccaa caagaatgtg ccacttaata ctaacgtagc gccaattccg
    13561 agaaaataac gcgattgtcc ctgacgtaca tgatttgact gaagctcgcg ggcaatctta
    13621 tcaacactgt gctgtaaata cttgccctgg cgcaaactgt cgtaaaccaa ttcaggcagt
    13681 tctggcattt tttcgaccca gaacggcgct ttttctttaa atgctctcac cagggcagga
    13741 ataccgacct gatctttaat ccacgactcc aggaaaggct tcgctgtttt ccataaatcg
    13801 agctgcggat aaagctggcg tcctacccct tcgacataga gcagggtttt ctggagtaac
    13861 accagttgcg gctgcacttc catattgaag cgacgcgccg tattaaacag atttaacagt
    13921 acatgtccaa acgaaatttc ggccagcggt ttctcaaaga taggttcaca gaccgtacga
    13981 atggcaaatt cgaactcttc aacgttggta tctggtggca cccagccaga atcgacgtgt
    14041 agctctgcca ctttgcgata gtcgcgatta aagaaggcga taaagttttc tgccagatag
    14101 cgtttatctt ctttgtttag cgagccaaca atcccgcaat caatgccgat atatttcggg
    14161 ttttccgggt gttcatagct tacgaaaatg ttgccagggt gcatatcggc atggaaaaag
    14221 ctgtcgcgaa agacctgagt gaagaacacc tgcacgccac gttccgccag caatttcatg
    14281 ttggtgccat ttttctccag cgtcgcaaca tcagacaccg gaatgccgta aatgcgctcc
    14341 atcaccatca tcccttcact acaatagtca gggtaaactt ccgggatgta gagcatcggg
    14401 ctgtcttcaa aatttcgccg aagctgaatg gcgttggcag attcccgcag caaattcagt
    14461 tcatcaatca aggtcttttc gtactcgcgc accacttcgg ttgggcgcag acggcgacca
    14521 tccggcagca aacgcggcac ccagcgagcc agacggtaga taagtttcag atccgcttta
    14581 ataaccggca aaatatccgg gcggatgact ttaatcacca cctctttacc attcgatttc
    14641 aatcgcgcgg tatgaacctg ggcgatagaa gcagaagcca gtggcttgat ttcaaaatcg
    14701 tcaaaccacg cttctaccgg caagccgccc attgcagctt caatctgctg cttcgccagc
    14761 ttgccatcaa acggagcaac tttgtcctgc aataacgcca gctgatcggc aatatgcggt
    14821 ggaaaaagat cgcggcgggt tgataacatt tgcccgaact tgatccaaac cggccccagt
    14881 tcttgcaggg ccagtcgtag tcgctcacct aaaagtttgt ctttatgccg atttggcatc
    14941 cagaataatg agtatcgcca tagccgtagc ggcagggtga tacgcatttt ggggatcagt
    15001 tcatcaagtc cgtagcttaa aaaagtgcga atgatgaaat ataggcgccg tacttcacct
    15061 ggcgtcattt agcctccagt ttttccagcc gtttggtcag ggcatcaaca gcacgctcga
    15121 cggcagccgt ctcttccgca aaccaggcca cttcaagcgg accgggtgcc atacgccact
    15181 cttcagtaat ggcttccgcc acataacgtt gctggcgctt aatgccgtga tgcaggaact
    15241 ttgcgcctcc gcgcatggct ttgctgattc cttcagcggc gatatcaccg gtataagggg
    15301 ccagcagttc cgcagggtcg aactctgcca gatctgccag cgcaacgaag ttttgcacca
    15361 cctgaatatc gccctgcact tccagctcac cactgcgaat cagtgcggta agctgctggc
    15421 gatcgcgaag tttcggcaac acactggcgt aggcgataac ggtgcagtca gcatcgcctg
    15481 cccattcgcc cagtacatca acctggcgtt cgctgaacac cagaatcaat gacgtcgaaa
    15541 agccttttac ctccacgcgc aatactttac ccagcagacg cgagcgggct gttttcagcg
    15601 cgggtgagcg atacaggaag gtgttgagca gactttcaat tcctgccgtc actaaaggtt
    15661 taaaaggcat ttccggtctc ctgtcagaac ttataaccac gatgcagcgc cacaacccct
    15721 gccgtcagat tgtagtagtc gacactttcg aatccggcat cctgcatcat ggctttcagg
    15781 gtatcctgat cgggatgcat acggatggat tctgccagat aacggtagct gtcggcgtcg
    15841 ttcgcgacca gtgagccaat acgcggcagc acatggaagg agtatgcatc ataggctttg
    15901 ctcagcggct cgataattgg cttcgagaac tcaagcacca gcaggcggcc gccgggtttc
    15961 agcacgcgat acattgaacg cagtgcttta tctttgtcgg tgacgttacg cagaccaaac
    16021 gaaatggtga tgcaatcaaa ggtgttatcc gggaacggca gcgcctcagc gttcgcctga
    16081 acatactcaa cgttgccaat cacaccgata ttacgcagct tctcgcgtcc cattttgagc
    16141 atggattcat tgatatcagc aaggaccact ttgccagttt ctccgaccag gcgggagaat
    16201 ttcgctgtca ggtcgccggt gccaccagcc agatccagca cggtctgccc acggcgtacg
    16261 ccgctgcaat caatcgtgaa tcgcttccac aaacgatgaa taccaaatga catcaaatca
    16321 ttcatgacat cgtattttga tgccacggaa tggaaaacgt gggcgaccat atccgctttt
    16381 tgttccttcg cgacggtctg aaaaccaaag tgcgtcgttt cttgtgactt atccaccatc
    16441 tcaatgcctg ctcatcaaaa aattgttcca gaagtgtaac agattgggcg tcgatgccct
    16501 agatttctac ccggcttaac tacccctaat gggctagcgc gactgctgat tatattcatc
    16561 atcgcgttga taagcttcat cattcggctg ctccggaacc gaccgaagtc gatactcttc
    16621 attctggctc accgcctgtt cagccaaatc cggattaatc tcgcgtttaa tttctactcc
    16681 taaaccgcga aacgcttctg cctgcgccag cacatttccg cgacctgaag agagtttttt
    16741 cattgcctgg cggtagttat cctgcgcttt gtcgagactt tgaccaatcg cggacatgtc
    16801 atcgatgaac aaacgcatct tgtcgtacag cttgctggca cgatcggcga tttgctgggc
    16861 gttgcggctt tgatgctcat aacgccacag gttggcaata gtacgcaacg ccaccagcag
    16921 cgtagtcggg ctaaccagca tgatattgtt tttcaacgct tcggtgatca gctccggctg
    16981 gcggtcaagc gccagtaaaa aagcgggttc aacgggaata aacatcagca cgtaatccag
    17041 agttcgcagc cccggcagct gttgataatc tttgcgtccc agcaaacgga tatggttacg
    17101 caccgacgcg atatgttcct gtagcgcgct ttcgcgggtg tagtcgtctt cggcgttaaa
    17161 atagcgttca taggcgacca gcgtcatttt ggcgtcgatc accacatctt ttccctgcgg
    17221 cagccgcacg atgacatccg gctgcatccg cgagcgggcg tcattttcga tgctgacctg
    17281 ggtttcatat tcataccctt cacgcagacc ggaagcctcc agcacccgcg tcaatactac
    17341 ctcgccccag ttgccctggg ttttattgtc gcctttcagc gcgcgcgtca ggttgatcgc
    17401 ttcctgggcc atttgcgcgt tgagttgctg gagattgcga atttcgtggg tcagggtatg
    17461 gcgttcttgt gcttctttac cgaagctgtc ctgaacctga cggcggaaac cgtccagttg
    17521 ttcacgtagc ggcgacaaca ggctgttcag actctgacgg ttttgctcat caacccggcg
    17581 attgctgtgc tcaaaaatac ggttggcgag gttttcaaac tgctcactga ggcgctgctc
    17641 gctgttaatc atctggcgaa ttttatcgtc agcatgttgc tgtgcggctt ccatccgcgt
    17701 ggttacttca cgcagatcgg cctccagaga ggtgttaata ctttgcaggc tgcgcacttc
    17761 gttattgagt aactcgcact ctgcacgcca gtgctcgctt tgggtaattt gttgttttgc
    17821 cgcgcttaac tccgcgacca tctcttcacg ttcagctaat tgctcggctt tttgctgcgc
    17881 atgttgataa ctggcaaaca gccagccaat tgccacaccc accaacgcaa taactgcgta
    17941 aaccatgatt gagaaatcca caacgcctcc tgacgcaaca acttacccgc acaaaataga
    18001 attgtgctgt acaaacgtcc agttggaaag gcatttcctg cgaggcgcta cgcaaaaata
    18061 caaaaggccg aacgcgtcgg ccttcagaca ggagaagaga attacagcag acgacgcgcc
    18121 gcttccacca cgattttcac cgcttggctt tcagtttgtt tcatcgtctc agcattcggg
    18181 atctcttgct gggtgcggtt aacgataaca cccgctacca taccggcacg caggccctga
    18241 cttgcacaca tggtcagcag ggttgcagat tccatttcat agttcattat gcccatcgcc
    18301 tgccactctt ccatagaacc tttaaagtga cgaactacgc gaccagagta agtatcgtaa
    18361 cgttcctgac ctgggtagaa ggtatcagaa gaagctgtca cgccaacgtg agttgtcgcg
    18421 ccaatggatt tcgcagcttc aaccagcgca gtcgtacatt cgaaatcagc gacagccggg
    18481 aattccagcg gtgcgaagtg caggctcgcg ccatccagac ggacagacgc cgtggtaacc
    18541 aggacatcac ccacattaat atgcggctga atagcgcccg ttgtaccgat acgcaggaag
    18601 gtgcgaatgc ccagctgtgc cagctcttca acagcaatag aggtagacgg gccgccgata
    18661 ccggtagagc agacgataac aggtttacca tccagctctg cacgccaggt agtgaattcg
    18721 cggtgagatg ccagcttaac cggcttatcc atcagcgcgg cgatcttttc cacacgatcc
    18781 gggtcgccag ggacgatggc aagcgtagcc ccttgtaaat cgtttttagt gaggccgaga
    18841 tgaaaaacat cagacttgga catatacaac tcctctgtga atcggtttag tcagaagaag
    18901 caaaaagaca ctttaccgaa gggtttaaca ttttttcgtg atactcatca ccatgacgca
    18961 aatgcgttgc ataaatttac ccaccaaaag tgattcaaat cacataaatc actcttcatt
    19021 ctgcaggact gcataaaaga tgagccgatt caggcaatgt gatttgttgc atgctgacta
    19081 tagttaacat tgcgcaacat ttttataagg gtacggagaa taccatggca acaacacaac
    19141 aatctggatt tgcacctgct gcatcgcctc tcgcttcgac catcgttcag accctggacg
    19201 acgcgattgt ggcgggcttc acctctatcc cttcacaagg ggataacatg cctgcttacc
    19261 atgccagacc aaagcaaagc gatggcccac tgccagtggt cattgtagtg caggaaattt
    19321 ttggcgtgca tgaacatatc cgcgacattt gtcgccgtct ggcgctggag gggtatctgg
    19381 ctatcgcacc tgaactttac ttccgcgaag gcgatccgaa tgattttgcc gatatcccca
    19441 cgttgcttag cggtctggta gcaaaagtgc ctgactcgca ggtgctggcc gatctcgatc
    19501 atgccgccag ttgggcttcc cgcaacggcg gcgatgttca tcgtttaatg atcaccggat
    19561 tctgctgggg tggacgtatc acctggctgt atgccgcgca taatccacag ctaaaagctg
    19621 cagtggcgtg gtacggcaaa ctgacgggcg acaagtcgct gaattcaccg aaacaacctg
    19681 ttgatatcgc aaccgatctt aacgcgccga ttctcggctt atatggcggt caggataaca
    19741 gcattccgca ggagagcgtt gaaaccatgc gccaggcgct gcgggctgct aatgcgaaag
    19801 cagagattat cgtgtacccg gatgccgggc atgcattcaa cgctgattat cgcccgagct
    19861 atcatgccga atctgcgaaa gatggctggc agcgtatgct ggaatggttt aagcagtatg
    19921 gagagaagcg gagttagttt cctcttagtc tatatttgag atggtgagta aacgagtgcg
    19981 ttttgctgcc atctctgtcc cccaaaaaat aatcagtatg aattgattgg agattacgac
    20041 tccgctattt gaccttaaga aagtcggcaa gtcatagtca taaatgagtc tcttcaatgg
    20101 aagacacctt agagatttac catcaactcc cattcacaaa caaacattaa ttattatcaa
    20161 cacattcaca ttaattataa atcatctcag accttcctga taaaaagata ttaaagtgaa
    20221 acattttgtt tttcacattt tagttcatca cttttaggcg taaataaagg taaaaaaaac
    20281 aacaatacta tttctctttc acaaaagaat taacaaaagg tttttcaagg atgagtgata
    20341 ttatttacct gaccataacc ggagaacaac aaggatgtat ttcctctcgt tgtggaacga
    20401 gcgcatcaat aggtaatcgc tggcagattg gccatgatga tgaaatcttc gcgttctcgc
    20461 tctctaatag tattacgaat acaggcaaag ggtcataatt gcatggcctt agtttctgta
    20521 aattgattga caaaagctcc ccgttactta ttaacgctat taacaataac gagcagttgt
    20581 tcatggagtt tgacttctat cggatcaata ggtttggtcg atgggagaaa tattacaaca
    20641 ttcaactccg cggcgcgctc ttatcagcaa tcaatcacct gtttactgag aacaatctgg
    20701 atacagaagc catcactgtc agttattaat atatactctc caggcatctg attgctaata
    20761 ctgaatttag ttattgggca tttcccgata actacaaccg tctgtttata ccacggccaa
    20821 aaactaaagc tggtaatggt ttcaaaacac ttaatagtaa agcggttggc agactgcttg
    20881 ctgctgtcgg catatacaat gggaatatcg aaggattcag ggaaacagca gacaagttgg
    20941 ggggagatgc acctgctggt tatgatcaat ttatggataa caaagggtta ctcatcgctg
    21001 gtgcttcaat agttgctgga gcagttctgg ggagaatgag actccctgat ttggaatcac
    21061 ttgagcattt cggagccaga ggagcagtat caggaagacc atttaatccc gagcttgccg
    21121 gtggtccaat agaaaacctg acgacagatg gcgtaacaat caacagggaa ggtatcgcta
    21181 ttgttgaaaa acatatagcc agatttggtc atgatcctgt aaatgaagtg atgatcaata
    21241 gactgaaaga cattgagaaa ggaaaaatcc ccccagaaca agtagatctt aacttctata
    21301 ctcatgagtg tcgggaatat cagcgttact gtaatcttgg ttgggaaaca ggacaacctg
    21361 atggtgacgc aggctatgca ctctggaatc atactggatt tgcccctata tttccagaca
    21421 tctgttatca cttaacccat tacaagcccg ctgcc
//
LOCUS       DAJVQG010000067        21431 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJVQG010000067 DAJVQG010000000
VERSION     DAJVQG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.067"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            1..1365
                     /gene="espL1"
                     /locus_tag="OQM83_002198"
                     /pseudo
     CDS             1..1365
                     /gene="espL1"
                     /locus_tag="OQM83_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1537..1644
                     /locus_tag="OQM83_002199"
     CDS             1537..1644
                     /locus_tag="OQM83_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1520459.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1697..2455)
                     /locus_tag="OQM83_002200"
     CDS             complement(1697..2455)
                     /locus_tag="OQM83_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCS1520460.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2742..3671
                     /gene="pfkB"
                     /locus_tag="OQM83_002201"
     CDS             2742..3671
                     /gene="pfkB"
                     /locus_tag="OQM83_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCS1520461.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3772..4062
                     /gene="ghoS"
                     /locus_tag="OQM83_002202"
     CDS             3772..4062
                     /gene="ghoS"
                     /locus_tag="OQM83_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCS1520462.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            4168..5028
                     /locus_tag="OQM83_002203"
     CDS             4168..5028
                     /locus_tag="OQM83_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCS1520463.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5069..5605)
                     /locus_tag="OQM83_002204"
     CDS             complement(5069..5605)
                     /locus_tag="OQM83_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCS1520464.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5752..6420
                     /gene="hxpB"
                     /locus_tag="OQM83_002205"
     CDS             5752..6420
                     /gene="hxpB"
                     /locus_tag="OQM83_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCS1520465.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLRDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6583..7173
                     /locus_tag="OQM83_002206"
     CDS             6583..7173
                     /locus_tag="OQM83_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1520466.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            7306..8697
                     /gene="tcyP"
                     /locus_tag="OQM83_002207"
     CDS             7306..8697
                     /gene="tcyP"
                     /locus_tag="OQM83_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCS1520467.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(8701..9517)
                     /locus_tag="OQM83_002208"
                     /pseudo
     CDS             complement(8701..9517)
                     /locus_tag="OQM83_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9794..10057)
                     /gene="cedA"
                     /locus_tag="OQM83_002209"
     CDS             complement(9794..10057)
                     /gene="cedA"
                     /locus_tag="OQM83_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCS1520468.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            10240..12501
                     /gene="katE"
                     /locus_tag="OQM83_002210"
     CDS             10240..12501
                     /gene="katE"
                     /locus_tag="OQM83_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCS1520469.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(12759..13517)
                     /gene="chbG"
                     /locus_tag="OQM83_002211"
     CDS             complement(12759..13517)
                     /gene="chbG"
                     /locus_tag="OQM83_002211"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCS1520470.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(13530..14882)
                     /gene="celF"
                     /locus_tag="OQM83_002212"
                     /gene_synonym="chbF"
     CDS             complement(13530..14882)
                     /gene="celF"
                     /locus_tag="OQM83_002212"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCS1520471.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14987..15829)
                     /gene="chbR"
                     /locus_tag="OQM83_002213"
     CDS             complement(14987..15829)
                     /gene="chbR"
                     /locus_tag="OQM83_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCS1520472.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(15837..16187)
                     /gene="chbA"
                     /locus_tag="OQM83_002214"
     CDS             complement(15837..16187)
                     /gene="chbA"
                     /locus_tag="OQM83_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCS1520473.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(16238..17596)
                     /gene="chbC"
                     /locus_tag="OQM83_002215"
     CDS             complement(16238..17596)
                     /gene="chbC"
                     /locus_tag="OQM83_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCS1520474.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
     gene            complement(17681..18001)
                     /gene="chbB"
                     /locus_tag="OQM83_002216"
     CDS             complement(17681..18001)
                     /gene="chbB"
                     /locus_tag="OQM83_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCS1520475.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(18300..18638)
                     /gene="osmE"
                     /locus_tag="OQM83_002217"
     CDS             complement(18300..18638)
                     /gene="osmE"
                     /locus_tag="OQM83_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCS1520476.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18840..19667
                     /gene="nadE"
                     /locus_tag="OQM83_002218"
     CDS             18840..19667
                     /gene="nadE"
                     /locus_tag="OQM83_002218"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCS1520477.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            19897..20784
                     /gene="cho"
                     /locus_tag="OQM83_002219"
     CDS             19897..20784
                     /gene="cho"
                     /locus_tag="OQM83_002219"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCS1520478.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(20744..21319)
                     /gene="ves"
                     /locus_tag="OQM83_002220"
     CDS             complement(20744..21319)
                     /gene="ves"
                     /locus_tag="OQM83_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCS1520479.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT         5703 a         5289 c         4908 g         5531 t
ORIGIN      
        1 cccttgattc attgagaaat ggaaaaggca aagtcaacct gattaaacac tactcctccg
       61 ttgaatccat acagcagcat gtccccttag tccgggacgc ggagttcaga gcattacttc
      121 gccatcctcc tgcagggagt cgcgttatcg cgagtaagga ttttggcttc gctttagata
      181 ttttcttttg tcgaatgatg gcaaacaatg tcagtcatat gtccgcgatt ttatatatag
      241 acaatcatac tttgtcagta aggctacgaa taaagcagtc agtgtatgga caattaaatt
      301 atgttgtgtc tgtttacgac ccgaacgata ccaacgttgc cgtcagaggc acccacaggg
      361 cagcacgggg ctttctctcg cttgataagt tcatcagttc agctcccgat gctcagacct
      421 gggctgatag gtatgttcgc aactgtgcaa ttgctattct tcctctatta cctgagggag
      481 ttccagttgc tattttagcg ggtatcacga cacgaatgcc atttgcccct atacatccat
      541 cggcaatgtt gttaataatg gccacaggcc agactcaata gcttattaca ctattcaaac
      601 agttgcccat actccctgaa aaagaaatca ttgaaataat aactgcgcag aatagcgttg
      661 gtacacctgc tttatttctg gctatgatga acggacaaac tgacaacgta aaaatattta
      721 tgcaagaaat tcagtcactg gtatacaatc atatcattca tgaagataat ctggttaaat
      781 tactgcaaac taaaagtgct aacgaaacac ctggactcta tatctccatg ttgtatggat
      841 tcgatgaaat aatcgatatc tttctgaatg cattaaccac tccaatagca caagaacttt
      901 taaacaaaaa aaatggtgat gagtatttta gccatgaaaa tacatgatgg tgagccagga
      961 ttatacgccg caatggaaaa taatcaccct ttgtgtgtca cacggttcct ctctaaaatt
     1021 aatggcatcg cctttaaata caagttgagc aaagctaaca tcatggattt attaaaagga
     1081 gctacagcac agggaacccc tgcattatac atcgccatga gcaagggtaa tgaagacgtc
     1141 gtgttatctt atatatcgac gctgggtgct tttgcaaaaa aacattcttt tagtcaacat
     1201 cagttattta cactattggc cgctaaaaat catgacaaca tgtcagctgt tcatatagcc
     1261 attcatcata agcattataa aactgtagaa acatattatg ctgctataaa tgtcatcagc
     1321 caaagcctga gttttagtgc tgatgaaata aagacgtatt tataaccagc tatttacgat
     1381 atatatcgat acataatttt atttttttca taaaacgcag aaacagtgac catgtgcaca
     1441 acgccacccg acggctgtaa taacaaaaaa tcccttgatg cctatccctt ttgttacact
     1501 ccgttatcac gcacaagaga tatgcaggac actggtatgc cgactaaacg ctttgataaa
     1561 aaacactgga agatggtggt ggtgctactg gcaatctgtg gcgctatgtt gttgctacgt
     1621 tgggcagcaa tgatttgggg ctgagcagtg acaaacgaag acagccagac cgcataacac
     1681 ggtccggcag ataattttac atgctgtagc ccagcgataa ggttgtccga cgatcggtat
     1741 gttccggtgc agactctgga ggttcagagt tccatgtgac gttgtaagcc accttcagtc
     1801 caaaatgttc attaatagca acatttaatg cgctttcgga gttcagcgtt gtatcttccg
     1861 cgccaaagac cgaaacaccc tgcgtaaatt tagcgttgtc agtcaactgc catgcataag
     1921 ccccggaggc atagcccagc ggctgggttt cactggcatt gtcggtatat ttgtcgtaac
     1981 gcacacctgg gccgaattca aagcggaaac tgtgtaccgg gccattcaaa aactggcgac
     2041 cataacccgc ggtcaacaca tcgcgctctc gatagccgtt ataacggtct gtcagccagc
     2101 ttgcctgacc aaataaatag tcataatcag ttaaattaaa acggctacgc ccgcccgccg
     2161 catatttttc tgaagaacgc tcatcattag aagaagtatt actggcgttc ccccacagcg
     2221 accaggcagt ggtttgtcca taccaggtca tggtggtgtc agccgtaagt gaggagcttt
     2281 tcgtattgcc tgattgtgca agatatcctg cgttcagatt accttcgaaa ggttttttag
     2341 cgctggcagg atcgtccatg acagtaaaaa cggaatcatc ggcagctgca ttcagtgacg
     2401 caaacatgcc ccccgccaac ataacgatgg caggaactgt cttcaaaagc ttcatttatc
     2461 aagagtccgt acaacaaaaa aagagaccat cgcggtcccg gaaactttct taaggatcaa
     2521 agattagcgt ccctggaaag gtaacgaatt ataaaaaggc gcgaataact tagcaatgta
     2581 ttcttatttc attttttgaa taagcatgtg gcgaaaacag atttttattt atatatattt
     2641 atctgcaaaa ttttaaataa agctccaata aatcatattg ttaatttctt cactttccgc
     2701 tgattcggtg ccagactgaa atcagcctat aggaggaaat gatggtacgt atctatacgt
     2761 tgacacttgc gccctctctc gatagcgcaa caattacccc gcaaatttat cccgaaggaa
     2821 aactgcgctg taccgcaccg gtgttcgaac ccgggggcgg cggcatcaac gtcgcccgcg
     2881 ccattgccca tcttggaggc agtgccacag cgatcttccc ggcgggtggc gcgaccggcg
     2941 aacacctggt ttcactgttg gcggatgaaa atgtccccgt cgctactgta gaagccaaag
     3001 actggacccg gcagaattta catgtacatg tggaagcaag cggtgagcag tatcgttttg
     3061 ttatgccagg cgcggcatta aatgaagatg agtttcgcca gcttgaagag caagttctgg
     3121 aaattgaatc cggggccatc ctggtcataa gcggaagcct gccgccaggt gtgaagctgg
     3181 aaaaattaac ccaactgatt tccgcttcgc aaaaacaagg gatccgctgc atcgtcgaca
     3241 gttctggcga agcgttaagt gcagcactgg caattggtaa catcgagttg gttaagccta
     3301 accaaaaaga actcagtgcg ctggtgaatc gcgaactcac ccagccggac gatgtccgca
     3361 aagccgcgca ggaaatcgtt aatagcggca aggccaaacg ggttgtcgtt tcccttggtc
     3421 cacaaggagc gctgggtgtt gatagtgaaa actgcattca ggtggtgcca ccaccggtga
     3481 aaagccagag taccgttggc gctggtgaca gcatggtcgg cgcgatgaca ctgaaactgg
     3541 cagaaaatgc ctctcttgaa gagatggtcc gttttggcgt agctgcgggg agtgcagcca
     3601 cactcaatca gggaacacgt ctgtgctccc atgacgatac gcaaaaaatt tacgcttacc
     3661 tttcccgcta acaaaaacat tcccccagca ttgggggaat caccaccaac ctgtcggcaa
     3721 cgcgtttctc cgactttgct caaaagtcat gtgataacaa aggggtgaac tatggccagt
     3781 ggcgatcttg tccgttatgt cataaccgtc atgttgcatg aggatacatt gactgaaatt
     3841 aacgagttga ataattacct gactcgcgac ggttttttgc tcaccatgac ggatgatgag
     3901 ggaaatatcc atgagctggg gactaacact tttggactta tcagtaccca aagtgaagaa
     3961 gaaattagag aactggtttc ggggcttacc caaagtgcaa ccggcaaaga tcctgaaatc
     4021 accatcacga cttgggagga atggaatagc aacagaaaat aaatggtttt tgggcaataa
     4081 tcagtctgtg gtgtgcgtta gctcgtgttt ttacaccgca ttcttgcgct aaccttatga
     4141 tctggcagac aacatgggag agacatcatg tggcaggcaa tcagtcgtct tttgagcgag
     4201 cagttaggtg aaggcgaaat cgaactgcgt aatgaactgc ctggcggaga agtccatgcc
     4261 gcatggcatt tgcgctatgc aggacatgac tttttcgtca aatgtgatga aagggaactg
     4321 cttcccggtt ttaccgccga agccgaccaa ctggagttac tgtcgcgtag taaaaccgtc
     4381 accgtgccta aggtttgggc agtaggcgct gaccgtgact acagttttct ggtgatggat
     4441 tatctcccac ctcgtccgct ggatgcgcat agcgcattta ttcttggtca gcaaattgcg
     4501 cgtttacatc aatggagtga ccaaccacaa tttggcctcg attttgataa ctcgctctcc
     4561 acaactccac agcccaacac ctggcaacgt cgctggtcaa cgttttttgc tgaacaacgg
     4621 attggctggc agttggaact ggcagcagag aaagggatcg ctttcggcaa tatcgacgcc
     4681 atcgtcgagc atattcagca gcgtctggcc tcgcatcaac tgcagccttc tctgttgcac
     4741 ggcgatttat ggtccggcaa ctgtgcgctg ggtccggatg gcccgtacat tttcgaccct
     4801 gcctgctact ggggtgaccg agagtgcgac ctggcgatgt taccgctgca tactgaacag
     4861 ccgccacaaa tctatgacgg ctatcagtca gtatccccgc tacctgccga tttccttgaa
     4921 cgtcaaccgg tttaccaact ctacacgctg ttaaatcgtg caagattatt tggcggtcag
     4981 catttggtta ttgctcagca gtcattggat agattattag cagcatgata tgggttgagg
     5041 ataatggccg ctccgtgcgg ccttttgatt aaataaaccc aagcagagaa aagaacacat
     5101 aacctgcagc aatacaaata accggcagga tatatagtga gaaaaactgc aggaaaatag
     5161 tatggtgcgg aacaacaata cgggactcaa tttgttcacg cgttagcccc tcttcccctt
     5221 tggctttttc cagaatgagt tgatcttcaa caccttcccg caagaagcgc gcctggcgac
     5281 tcatccgggc accggaatcc tgcaacgcca gtccgacaaa aatcaggatg aaaatcaccc
     5341 agaacataac gttcacgcca ccattaaaat taggggtcgg ggagttatac cagaacagat
     5401 tcaaaaaagg cgtattcgcc tgcatcatat cgatcatgac atgagtaaag tcgagcatga
     5461 ccgcattaat gccttcctgg ttttcctgcc gggtattcat aaatttcagc aaagaaatca
     5521 atgtggagat caatgcaggt ataaaaatca cccaacccaa aatccttttc aaaacagcaa
     5581 tgcgtccagc ttgttgatac gtcatgagtt ctccttgatt aagacgcgtc gtttcactta
     5641 gtttacctgt agatatctgt tttcgcccat tctttaaagg cgatatgata ggcgcttaat
     5701 cataagcacg gcttaatacc ttacacataa tgctctaaag gagaggttgt aatgtcaacc
     5761 ccgcgtcaga ttcttgctgc aatttttgat atggatggat tacttatcga ctcagaacct
     5821 ttacgggatc gagccgaact ggatgtgatg gcaagcctgg gggtggatat ctcccgtcgt
     5881 aacgagctgc cggacacctt aggtttacgc atcgatatgg tggtcgatct ttggtacgcc
     5941 cggcaaccgt ggaatgggcc aagccgtcag gaagtagtag aacgggttat tgcccgtgcc
     6001 atttcactgg ttgaagagac acgtccatta ttaccaggcg tgcgcgaagc tgttgcgtta
     6061 tgcaaagaac aaggtttatt ggtgggactg gcctccgcgt caccactaca tatgctggaa
     6121 aaagtgttga ccatgtttga cttacgcgac agtttcgatg ccctcgcctc ggctgaaaaa
     6181 ctgccttaca gcaagccgca tccgcaagta tatctcgact gcgcagcaaa actgggcgtt
     6241 gacccactga cctgcgtggc gctggaagac tcggtaaatg gcatgatcgc ttctaaagca
     6301 gcccgcatgc gttccatcgt cgttcctgcg ccagaagcgc aaaatgatcc acgttttgta
     6361 ttagcaaacg tcaaactctc atcgctgaca gaactcaccg caaaagacct tctcggttaa
     6421 tgaccagggg cagtgatcac gctgcccctg gttctttatc tgaatttgca ttcaactgac
     6481 ggattaatcg tcaatttaag agaaagagtt acaccgtcac cacttccgtg cactgtataa
     6541 aaatcctata ctgtacgtat cgacagttta gtgagtttta tcatgacggc ggaaggtcac
     6601 cttctctttt ctattgcttg tgcggtattt gccaaaaatg ccgagctgac gcccgtgctg
     6661 gcacagggtg actggtggca tattgtccct tccgcaatcc tgacgtgttt gttaccggac
     6721 atcgatcacc caaagtcgtt tcttgggcag cgattaaaat ggatatcaaa accgatcgcc
     6781 cgcgcttttg ggcatcgtgg ttttacccac agtctgctgg cggtatttgc gctgctggca
     6841 accttttacc ttaaggttcc ggaaggctgg ttcattccgg ctgatgcgct acaaggaatg
     6901 gtgctgggtt atttgagcca catacttgcc gatatgctga cacccgccgg tgttcccctg
     6961 ctctggccat gccgctggcg tttccgcttg cctatcctgg ttccccaaaa gggcaaccaa
     7021 ctggaacgtt ttatctgcat ggcattattt gtctggtcgg tatggatgcc ccattcatta
     7081 cccgagaaca gcgctgttcg ttggtcatcg caaatgatca ataccttgca gatccagttt
     7141 catcggctta ttaagcatca ggttgaatac taaaaaggca aaaatcacct ttctggaata
     7201 agcaattcca tttgaatata agagccagct cacagttctg ttaatcttgc gccaacacta
     7261 tgactgctac gcagtgatag aaataataag atcaggagaa cggggatgaa ctttccatta
     7321 attgcgaaca tcgtggtgtt cgttgtactg ctgtttgcgc tggctcagac ccgccataaa
     7381 cagtggagtc tggcgaaaaa agtgctggtg ggtctggtga tgggtgtggt ttttggcctt
     7441 gccctgcata ccatttatgg ttctgacagc caggtactta aagattctgt acagtggttt
     7501 aacatcgttg gtaacggcta tgttcaactg ctgcaaatga tcgttatgcc gttagtcttc
     7561 gcctctattc tgagcgcggt tgcccgtctg cataacgcat ctcagttagg caaaatcagt
     7621 tttctgacca tcggtacgct tttgtttacc acgctgattg cggcgctggt cggtgtgctg
     7681 gtcaccaacc tgtttggttt gacggctgaa ggtctggttc agggtggtgc agaaactgca
     7741 cgtctgaacg ccattgaaag taactatgtt ggtaaagtct ctgatctgag cgttccgcag
     7801 ctggtcttgt cctttatccc gaaaaacccg tttgccgatc ttaccggagc caatccgacg
     7861 tcaattatca gcgtggtaat ttttgccgca ttcctcggcg tagctgcatt gaaactgctg
     7921 aaggatgatg cgccgaaagg tgaacgcgtc ttagccgcta tcgataccct acaaagctgg
     7981 gtgatgaaac tggttcgcct tgtcatgcag ttgacccctt acggcgttct ggctctaatg
     8041 accaaagtgg ttgcaggttc taacttgcaa gacatcatca aactgggaag tttcgttgtc
     8101 gcgtcctacc tcggtctgct gattatgttt gcagtgcatg gcattctgct gggcattaat
     8161 ggcgtgagtc cgctgaagta cttccgtaag gtatggcctg tgctgacgtt tgcctttacc
     8221 agccgttcca gtgctgcgtc tatcccactg aatgtggaag cacaaacgcg tcgtctgggc
     8281 gttcctgaat ccatcgccag tttcgccgcc tctttcggtg caaccattgg tcagaacggc
     8341 tgcgccggtt tgtatccggc aatgctggcg gtgatggttg cgcctacggt tggcattaac
     8401 ccgctggacc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag ttccgcaggc
     8461 gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc tgcgatgggc
     8521 ctgccagtaa ccctggtggc gctgttaatc tccgttgaac cgcttatcga catgggccgt
     8581 acggcgctaa acgttagtgg ctcgatgaca gctggcacgc tgaccagcca gtggctgaag
     8641 caaaccgata aagccattct ggatagcgaa gacgacgccg aactggcaca ccgttaattc
     8701 ttatgctggc aacggtccgt tttgtatagg ggccgttgct ttacttttta aatgttccat
     8761 cgcggcgatg catactgact catatttaaa cagatcgtca gcgaaaaaaa tgtccaactt
     8821 atcaatacat tcctggctca atgaatgtgc ctgtagtgag aagcttaaaa cctcataccc
     8881 gagacgtttt ctaagtgaac tatctggaat actcataata tcttcaagca caccacttcc
     8941 cattcccgga agatttagtg gattaaaaag aagattgata ttaagcattg atattatcga
     9001 aggaaacttt aggagactct catttttttt gaatcgcagc aaaaatgctc ccggcaggat
     9061 gtatataatt tttttcatag tatatagtcc ttaaataatg aataaccaac ctatttttta
     9121 tattgatcaa attcgtcatt ttttcgggtc ggaattcaca ggtaaaatcc atcccaacaa
     9181 aattctcatt aatatctctt atccgaaact cactgccata aagattatta attatcactc
     9241 tatatttaac attgtcctgc agatacgtat caaaactaag tgctggagct cctggctttg
     9301 aggcatcata gttaatgtta gacatatagg caatcatacg attgaagttc gactctgcta
     9361 tttcatcgca gtcacattga cccgttgata taaagaaagt gagtgccgcc atggtagact
     9421 caagcctttc tttaaaagag tcgaaagcca ggcttttaat atttaaatca ccataattac
     9481 tctgtattaa gtttgtagaa aacatctccc gccccatatt gttaacaaaa ttattatctc
     9541 atttaaatct aagtcactta caatataagt ttaagagcga cgccacagga tgaactatca
     9601 aaaatagctc atcatgatta gcaaaactta accattttaa aataaataaa caattaaaga
     9661 aaaaagatca cttatttata gcaatagctc gtcaaaggca gctttttgtt acaggtggtt
     9721 tgaatgaatg tagcaacgaa atgcagaatt tcaggtcatg taactcccgg caaaaccggg
     9781 aagtatgtaa tccttactca gtcacttccc cttcctggcg gatctgattt gcccaacgtt
     9841 gggcagattc aggcacagta aacgccggtg agcgcagaaa tgactctccc atcagtacaa
     9901 acgcaacata tttgccacgc agcatccaga catcacgaaa cgaatccatc tttatcgcat
     9961 gttctggcgg cgcgggttcc gtgcgtggga catagctaat aatctggcgg ttttgctggc
    10021 ggagcggttt cttcattact ggcttcacta aacgcatatt aaaaattaga aaaactgtag
    10081 tttagccgat ttagcccctg tacgtcccgc tttgcgtgta tttcataaca ccgtttccag
    10141 aatagtctcc gaagcgggat ctggctggtg gtctatagtt agagagtttt ttgaccaaaa
    10201 cagcggccct ttcagtaata aattaaggag acgagttcaa tgtcgcaaca taacgaaaag
    10261 aacctacatc agcaccagtc accactacac gattccagcg aagcgaaacc ggggatggac
    10321 tcactggcac ctgaggacgg atctcatcgt ccagcggctg aaccaacacc gccaggtgca
    10381 caacctaccg cccctgggag cctgaaagcc cctgatacac gtaacgaaaa acttaattct
    10441 ctggaagacg tacgcaaagg cagtgaaaat tatgcgctga ccactaatca gggcgtgcgc
    10501 atcgccgatg atcaaaactc actgcgtgcc ggtaaccgtg gtccaacgct gctggaagat
    10561 tttattctgc gcgagaaaat cacccacttt gaccatgagc gcatcccgga acgtattgtt
    10621 catgcacgcg gatcagcagc tcacggttat ttccagccat ataaaagctt aagcgatatc
    10681 accaaagcgg atttcctctc agatccgaac aaaatcaccc cagtatttgt taggttctct
    10741 accgttcagg gtggcgctgg ctctgcagat accgtgcgtg atatccgtgg ttttgccacc
    10801 aagttctata ctgaagaggg tatttttgac ctcgttggca ataacacgcc aatcttcttt
    10861 atccaggatg cgcataaatt ccccgatttt gttcatgcgg taaaaccaga accgcactgg
    10921 gcaattccac aagggcaaag tgcccacgat actttctggg attatgtttc tctgcaacct
    10981 gaaactctgc acaacgtgat gtgggcgatg tcggatcgcg gcatcccccg cagttaccgc
    11041 accatggaag gcttcggtat tcacaccttc cgcctgatta atgccgaagg gaaggcaacg
    11101 tttgtacgtt tccactggaa accactggca ggtaaagcct cactcgtttg ggatgaagca
    11161 caaaaactca ccggacgtga cccggacttc caccgccgcg agttgtggga agccattgaa
    11221 gcaggcgatt ttccggaata cgaactgggc ttccagttga ttcctgaaga agatgaattc
    11281 aagttcgact tcgatcttct cgatccaacc aaactgatcc cggaagaact ggtgcccgtt
    11341 cagcgtgtcg gcaaaatggt gctcaatcgc aatccggata acttctttgc cgaaaacgaa
    11401 caagcagcat tccatccagg tcatattgtt cctggtctgg acttcaccaa cgatccgctg
    11461 ttgcagggac gtttgttctc ctataccgat acacaaatca gtcgtcttgg tgggccgaat
    11521 ttccatgaga ttccgattaa ccgtccgacc tgcccttacc acaacttcca gcgtgatggc
    11581 atgcatcgta tggggatcga cactaacccg gcgaattacg aaccgaactc gatcaacgat
    11641 aactggccgc gcgaaacacc gccgggaccg aaacgcggcg gttttgaatc atatcaggaa
    11701 cgcgtggaag gaaataaagt tcgtgagcgc agtccatcgt ttggcgaata ttattcccat
    11761 ccgcgtctgt tctggctaag tcagacgcca ttcgagcagc gccatattgt cgatggtttc
    11821 agttttgagt taagcaaagt cgttcgtccg tatattcgtg agcgcgttgt tgaccagctg
    11881 gcgcatattg atctcactct ggcccaggcg gtggcgaaaa atctcggtat cgaactgact
    11941 gacgaccagc tgaatatcac cccacctccg gacgtcaacg gtctgaaaaa ggatccatcc
    12001 ttaagtttgt acgccattcc tgacggtgat gtgaaaggtc gcgtggtagc gattttactt
    12061 aatgatgaag tgagatcggc agaccttctg gccattctca aggccctgaa ggccaaaggc
    12121 gttcatgcca aactgctcta ctcccgaatg ggtgaagtga ctgcggatga cggtacggtg
    12181 ttgcctatag ccgctacctt tgccggtgca ccttcgctga cggtcgatgc ggtcattgtc
    12241 ccttgcggca atatcgcgga tatcgctgac aacggcgatg ccaactacta cctgatggaa
    12301 gcctacaaac accttaaacc gattgcgctg gcgggagacg cgcgcaagtt taaagcaaca
    12361 atcaaggtcg ctgaccaggg tgaagaaggg attgtggaag ctgacagcgc tgacggtagt
    12421 tttatggatg aactgctaac gctgatggca gcacaccgcg tgtggtcacg cattcctaag
    12481 attgacaaaa ttcctgcctg atgggaggcg gcgcaattgc gccgcctcaa atgatttaca
    12541 tagtgcgctt tgtttatgcc ggatgcggcg tgaacgcctt atccggccta caaaactgtg
    12601 caaattcaat atattgcagg aaacacgtag gcctgataag cgaagcgcat caggcagttt
    12661 tgcgtttgtc agcagtctca agcggcgcag ttacgccgcc tttgtaggaa ttaatcgccg
    12721 gatgcaaggt tcacgccgca tctggcaaac atcctcactt acacatcccg ataactcccc
    12781 aaccgataac cacgctgagc gatagcacct ttcaacgacg ctgatgtcaa cacatccagc
    12841 tccgttaagc gtgggaaaca gtaagcactc tgacggatag tattatcgat aaacgccgga
    12901 tgacacatca cctccagcga acgatcaccc cgatggcttg cgtcatccag cacttgcagg
    12961 aacagtgcct cgctaatctc ttcaccataa aacgcgctac tgaatccctg agaactgcgc
    13021 agattagccg gtaaatcacc agagttagat agcggctgac ggtcgatgcg caacgcaatc
    13081 ccctcttcag ccgcaaacct ggcaacaatc gggaaaatct gcgggaacat atgcacatga
    13141 tgatggctat caagatgcgt aggtttgcgt ccaaatagct caatgaaacg caaatactga
    13201 ctggcaagct cctgagtaat ttcttccagc ggtaaagcat cttcttctgc caactgccag
    13261 atccattttc ccagcacacc atcgcgggtt aaccccggca tagctgtcag tggcttgccc
    13321 atagtaagga caaagtacat ccctatggcc agactcggtt catcacgact caactgcacc
    13381 gcatggtcaa tagcctgccc attcaccagc gccgtcgtcg atgtgacaat cccattgcga
    13441 caggcctcga taatgccgta gttctgacct ttgcttaagc caaaatcatc ggcattaaca
    13501 atcagtaagc gttccataat cagcctcggt taatgtgctt ttttaagctc tgcgatgcag
    13561 tcggcaaaat ttggcagcca tttctcgtgc gccagaatca tctcgcgtgc cagcagctca
    13621 gcatcgcgat cggaatgcac caacggacta aggtttagcg ccagtaacac atcgttaaat
    13681 tctccgctaa gtgcggcgtt gctggcagca atctcgaagc ctttaatggt gtgaatcagc
    13741 cccatcactt tatcatcgaa atgcgtaatg cgcggatgtg gcgtcgcgcc atcgcgcccc
    13801 agcttacagg tcatttctac cgcccagtct gccggaatat tatcaatatg cccatgatgc
    13861 gggatattaa cgtaatgttc agcttgcttg tcgttgtaga tagcgttgat cacttcgcac
    13921 gctgcatcag agtaataagc cccaccgcgc tgttccagtt ctttcggctt aactttcaac
    13981 tccgggtttt tatacagctc aaaaagttgt ttctcgactt tctgtactac ctgcgctcgt
    14041 gcgccgcctt tgtagtattc gcccatttca atagccagca tctctttttg cttgaagtaa
    14101 taaagcaaat aagaacacgg caacagattc agagaacgaa ttaagccttc gctaaatggc
    14161 agatcgaaaa tatttttaac gccagatgct tttaactgcc cggatgccac accatcaagc
    14221 aattcggcaa agcgcgactt gccatttacc agcacatcct taatgaacac catatggttg
    14281 aggccgaaca aatcgataga taaatcatcg ctgtctttca gcatcagaac atcgtgaata
    14341 aacatcttca tgccgatcgg aatattacac acgccgataa agcgtttaaa tccggtatga
    14401 cgataaacgg cttcagtaac cattcccgcc gggttagtga agttaatcac ccatgcattc
    14461 ggacaaagtt cttcgacatc ttttacgatg tcaaaaatca ccggaatggt acgcagacct
    14521 ttaaacagac cgcccgcgcc gttggtttcc tggccaagat aaccatgact taatggaata
    14581 cgttcatcca gttcgcgcgc cggtaattgg cctacgcgca gttgggtagt aacgaaatca
    14641 gcatctttca atgcttcgcg gcgatccagc gttttataaa gcttcatcgg gacgccagcg
    14701 ttatcaatca tccgttggca gagttcaaaa atgatatcca gtttctcttt accatcttcg
    14761 acatccacca gccataattc gctgaccggc aattcgtgat aacgcttaat aaatccttcc
    14821 agtaactccg gggtatagct gctcccgcca ccaatagtga cgacttttaa tttctggctc
    14881 ataatttctc ccttcagtac agaatactga tatctggcat atctgccccc ccggacataa
    14941 ataatccagc aacaggacag atatgtgaat tgtcaggtat aacgacttac tgattaaatt
    15001 cagtcaattt cttacgatag ctcttaggcg taaaggacgt taatttctta aacgttttaa
    15061 taaacaaact cgggctacta taaccggcct caaacgcaat atccgtcacg gaatagttgg
    15121 tcatttccag ttgttttttg gcaaaattaa tacggatctc attaataatc tgcattggtg
    15181 ttttgccata atatcgttga gtcgctcgcg tcaaatattc ctgtgatttg gctgacaacg
    15241 tcaccatatt ctccagcgcc gattcactaa actgctcttt atcatgcatc ttttctaccg
    15301 tacttttcag ccactgcggt acatcatcaa tcacctgttc ttcgcggtaa tgacgtaaac
    15361 ggttaatgac ataaaaagta accatctcaa caaactcttc cagccctgtt tcgcggaaat
    15421 tcaatgaaga aatcactgtt tccacatagg tgagaaaagc gttattggtc cggtatacct
    15481 gcgaagcgac aaagcaataa ggcaacaacg gcaggtaatg ctgctcaaaa aagcgtttac
    15541 tgatcccaac gttcaatata cgcgtggcac caaactcata aaaactttga tggtgcgaac
    15601 ctaacggaat aaaaacaaaa tcgccccgtt ccagtaacac gcgcttaccg ttaatctcct
    15661 ggaaataacg cccggttaat accagagtaa attcataata gtcgtgctga tgcagcccgc
    15721 tgatactctc agttttgtta tagataaaca catggaagtt tttgccatta aacaactgct
    15781 gttctcgggc agtggcaatt tccggcgcgt taatcactgg ctgcatcatc gactccttat
    15841 gccttcagtt tttcatgaag ctcaattaat tcagtaatca gttcacgcgc aagcatggac
    15901 gtcattaaat gatcctgagc gtggaccagc accagactca ctttcatctt accttcgccc
    15961 gcatcgcctt caatcagttt cgtctgtacc agatgcgctt cattcaatgc catccgtgac
    16021 tgatccatca tggcttttgc tgcagcaaaa tcgccctgct tcgcctgttt cagtgccgca
    16081 tacgccaggc tgcgcgcttg tccggagttg atgataagcc ccatcaccac ttcttccagc
    16141 tcttcagctt ccgtttgcgt atcgggaatg ttatcgagat ccatcataca tcgttcctct
    16201 ctattcttac cggcacgatt acccgtaccg gcatcgatta aaatttcagg gcgttagcga
    16261 tatcttcttc gcgctcttct ttatcaatcg cgttttgtgc tttgttagcc accacaacaa
    16321 agggcagata aattaacgtt gcgataccaa ggttgaagag tgcaaccagc aatgcggcga
    16381 cgctaccgtt ggtgttaaag aaggctccca gaccggttgg catggtccac ggtgcaatat
    16441 tggtcaccgg aggaatgatg cccatgtagt acgctgcgag ggtgattgcc gccagaatcg
    16501 gttgtaccag tacaaacggg ataaacatca ccgggttcat gataattggc agaccaaaca
    16561 gaatcggttc gttaatctgg aagatgccgg acggcagcgc cagttttgcc acctgacgat
    16621 aatcagcacg gcgagaggcg ataaagatag ccaggatcag gcctaaagtc gcaccactgc
    16681 ccccaaggaa aataaaggag tccagcatcg gcttggccca gatatggaag gtcttaccgg
    16741 ctgccagcgc cgcttcgacg gaaccatatt gctgataggt cgcgatattt tccagcgccc
    16801 acggcgtcat aatgccgttg tccagtgcgg tcagcgccag cgcgccatga ataccgaaga
    16861 accagagcag tggaacaaag atcacatagg cccagcccac cacgctaccc aacgatgcca
    16921 gtggggttga gatggtatcc ataatgatct gatggaagtt ggtgccccag gtattcaacg
    16981 cccaggcaat aatccccatc acggaaagaa taataaagcc gggaattaat gccgagaagg
    17041 aacgcgatac tgaagcaggt acgctatcgg gcaatttaat gacccaattg cggcggacaa
    17101 taaaggtaaa catttctgcc accaccaggc caataataat cccggagatg atattcgctc
    17161 cacctaacca gtttgcgcca accacatagg cctcaccgac actatatggg gtgacggtca
    17221 taaatgctgc aacggataac aacccagccg ccagcgcatc gactttacgc tcttctgcca
    17281 gcgccatgcc aataaagaac ggtgccatta aagacattat ccctaatgtt ccgttatata
    17341 cgttgccgcc aatacctttc aggccattaa gtgtttcaat ggttgaggcg tcgaggcgaa
    17401 tacctaagga ataaaaaaac gccccctccc caaagcttag aaaaacgttg ttaattaata
    17461 caaacatggc ccccgcaagg gttaacggca ttaagcgaat aaagccattt ttgattgcat
    17521 taacgtgtgg ctgctttcct attttaactg caaaagggag gagtaccttt tcaagcgatg
    17581 caataacatt actcatagaa aaataccctt aaaaaccgca atttaaatat tgcggtattg
    17641 atttatgaaa taactctttg acgggaaaat ttaaaataaa ttaatttgct gcggcttttt
    17701 taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc gactttgcca taaagcagcg
    17761 agtcaattac ttcaaccggt ttgttgggta acaaacgctg gatttcgggc aacatataag
    17821 caatctgcgg ccctaataac acgacatcgg cattctgacc tttttcacca gccagtgttt
    17881 ccggaaatgc ttcaataatg accggaactt catatttttc tgcctgtgcg cgcatttttg
    17941 ataccagtaa agaggtagac atgcccgcag aacaaaacag ataaatgtgt ttcttttcca
    18001 taaaactgcc ctcatcgacg attatctgtc agccagacac tccgcaagcc ttaacctgct
    18061 tccatgctct gggtaacttg cgaaaccaac atgacttttt ttgtggctga aaggagtata
    18121 cggtatagac ctgagcaata gtatttcttt gaccttataa attgtttctc cttgacctgt
    18181 ggttatgacc ctcttcacat ttcgggcaaa taattcgcga agataattaa gaactcaggc
    18241 caaaaaaaac cggcgcaatg gccggtttcc gttgttactc aagctttcag acgaattgat
    18301 tacttcgcag cctgtggatc agtgtcgtat tcagcacagg tctgataacc ggagttgatg
    18361 acatgtccgg tatcatctaa cgcgacaaag taggtttctg ctttaccatc acgttgaccc
    18421 aggatgtagg tctggcaagt accacgagca tggatcatgc tcacttcaga cgaaggttta
    18481 cccgcaattt gtgcaacctg cgcccggctc atgccttttt tgacgtcttt caccacaggc
    18541 tgtacaaact ggtctttagt acgatcataa gccgtacaac ccgccagcat ggttaatacc
    18601 gccgctgcac tcagaattcc tgccatattc ttgttcatat tccgtcctct tgtttatcag
    18661 cgtgttagat aagcctggaa tacattgggc gctttttcaa gcccgcgaac gaaacggctc
    18721 cgctttcaga ggattcctgt atgacgtttt aaccaccatt cagcccgctg tcgcttgtcg
    18781 tttcagtggc aacgggttag ctttaaggaa gttttgtctt ttctgtctgg aggggttcaa
    18841 tgacattgca acaacaaata ataaaggcgc tgggcgcaaa accgcagatt aatgctgaag
    18901 aggaaattcg tcgtagtatc gattttctga aaagctacct gcaaacttat ccgttcatta
    18961 aatcactggt gctcgggatc agcggcggtc aggactccac gcttgccgga aagctgtgcc
    19021 agatggcgat taatgagctg cgcctggaaa ccggcaacga atcactgcaa tttattgccg
    19081 tacgcctgcc ctatggtgtt caggccgacg aacaagattg ccaggatgcc attgccttta
    19141 ttcaaccgga tcgcgtatta accgttaata tcaagggcgc ggtattggcc agcgaacagg
    19201 cattgcggga agcaggcatt gaactgagcg attttgtccg tggcaatgaa aaagcgcgtg
    19261 agcggatgaa agcacaatat agcattgcgg gtatgaccag cggtgtcgtg gtgggcaccg
    19321 atcatgcagc agaagccatt accggattct tcactaaata tggtgacggc ggtacagaca
    19381 ttaatccgct gtatcgtctc aacaaacgtc agggtaaaca gttactggcg gcattaggtt
    19441 gcccggaaca cctttataag aaagcgccaa cggccgatct ggaagatgat cgcccttctc
    19501 tgccagatga agtggcactc ggcgtgacct atgacaatat cgacgactat ctggaaggga
    19561 aaaacgtacc tgaacaggtc gccagaacaa tagagaactg gtatctgaaa accgaacata
    19621 aacgccgtcc gccaattacc gttttcgatg atttctggaa aaagtaataa tttgcacata
    19681 atggtttgtg cgaaaaagag taatttgttc acgccggatg cggcgtgaac gccttattcg
    19741 acctacaaaa ctatgcaaat tcaatatatt gcaggagtcg aataggcctg acaggcgtag
    19801 cacgtcagac ggtgtaacct ttgtcatcga cccgcctctt ttttaatcgc tccccgcctg
    19861 ttacactgga tagataacca gcattcggag tcaacagtgg tacggcgttt aacttctccg
    19921 cggctcgaat ttgaagctgc ggcaatttat gaatatcccg aacatttacg ttcattcctt
    19981 aatgacttac ccacccgacc aggggtgtat ctgtttcatg gcgaaagtga caccatgccg
    20041 ctctatatcg gcaaaagcgt taatatccgc agtcgggtgc tttctcattt acgcaccccg
    20101 gatgaagccg ccatgctacg gcaatcccgg cgaattagct ggatctgtac cgcaggcgaa
    20161 atcggcgctc tgctccttga agcgcgatta atcaaagaac aacagccgct gtttaataaa
    20221 cggttgcgcc gcaatcgcca gctctgtgcc ctgcaattaa atgaaaagcg cgtcgatgtg
    20281 gtgtatgcca aagaggtgga tttttcacga gcccccaacc tgtttggcct gtttgccaat
    20341 aggcgcgcag ctttgcaagc attgcagacc atcgctgatg aacaaaaact ttgttatggc
    20401 ctgctgggac tggaaccgtt aagtcgcggt cgtgcatgtt ttcgttcagc gctaaaacgt
    20461 tgcgccggag catgctgcgg taaagagagc catgaggaac atgcgctacg cttgcgccag
    20521 tctctggagc gtttgcgggt ggtgtgttgg ccttggcaag gtgcggtggc actgaaagaa
    20581 cagcacccgg aaatgactca atatcatatt attcaaaact ggctgtggct gggggcggtt
    20641 aattcgctgg aagaagcgac aacgttaatt cggacacccg ccgggtttga tcacgacggt
    20701 tataaaattc tttgtaagcc gctgctttcc ggtaactatg aaattactga acttgatccg
    20761 gcgaatgacc agcgagccag ttgatttcac tgaacaacag tttaccttgc ggttgtaaaa
    20821 gccgcagggt gtgcctgcca tcgaaccagc acgcgccctg gtcggtagtg agcaatttat
    20881 cgcccagttg ccacgccccg ttaatcacaa acaccacccc gccgcgtgag ccaaaggtag
    20941 taaacgtacg ttcggcaatt ctgactttcg ccttacagac atccaggcgt gtcatgatgt
    21001 tgaaatccat cgacatctgc ccttccgtca gtttcgcttt taccacctgg tccgctgcaa
    21061 aggcaaaagg ctgtaacggt tttaaggtat ggttaaagcg gtctgcgctt tcaaggagca
    21121 tctcgccgcc ttccagcaac gtcactatcc tttccatgcc gggaaacaaa gaaaactccc
    21181 cattcgccgc gatggatgca atgctggcac gccagtaaaa atcacgtttt gccggtggga
    21241 aagtgcaaat ttcgcgcgtt tcaccggcag catttcgcca cagattcacc gacattttac
    21301 gcatatcaaa gtattccata ctcgctcccg gcccgttctt ttcattgtta tcgttcaggt
    21361 aaccgtggat ggttatcggc aactcgggtg atttacgtta gtggtgatca ggcttttttt
    21421 acaactgttt g
//
LOCUS       DAJVQG010000068        21010 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJVQG010000068 DAJVQG010000000
VERSION     DAJVQG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21010
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.068"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..172)
                     /locus_tag="OQM83_002221"
     CDS             complement(<1..172)
                     /locus_tag="OQM83_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1520480.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            344..1564
                     /gene="coaBC"
                     /locus_tag="OQM83_002222"
     CDS             344..1564
                     /gene="coaBC"
                     /locus_tag="OQM83_002222"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCS1520481.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1542..2000
                     /gene="dut"
                     /locus_tag="OQM83_002223"
     CDS             1542..2000
                     /gene="dut"
                     /locus_tag="OQM83_002223"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCS1520482.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDSTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2107..2703
                     /gene="slmA"
                     /locus_tag="OQM83_002224"
     CDS             2107..2703
                     /gene="slmA"
                     /locus_tag="OQM83_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCS1520483.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2740..3381)
                     /gene="pyrE"
                     /locus_tag="OQM83_002225"
     CDS             complement(2740..3381)
                     /gene="pyrE"
                     /locus_tag="OQM83_002225"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCS1520484.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3447..4163)
                     /gene="rph"
                     /locus_tag="OQM83_002226"
     CDS             complement(3447..4163)
                     /gene="rph"
                     /locus_tag="OQM83_002226"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCS1520485.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4290..5153
                     /locus_tag="OQM83_002227"
     CDS             4290..5153
                     /locus_tag="OQM83_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCS1520486.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5375..6199
                     /gene="dinD"
                     /locus_tag="OQM83_002228"
     CDS             5375..6199
                     /gene="dinD"
                     /locus_tag="OQM83_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCS1520487.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6491..7108
                     /locus_tag="OQM83_002229"
     CDS             6491..7108
                     /locus_tag="OQM83_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCS1520488.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7105..8787)
                     /gene="ligB"
                     /locus_tag="OQM83_002230"
     CDS             complement(7105..8787)
                     /gene="ligB"
                     /locus_tag="OQM83_002230"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCS1520489.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEIARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9045..9668
                     /gene="gmk"
                     /locus_tag="OQM83_002231"
     CDS             9045..9668
                     /gene="gmk"
                     /locus_tag="OQM83_002231"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCS1520490.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9723..9998
                     /gene="rpoZ"
                     /locus_tag="OQM83_002232"
     CDS             9723..9998
                     /gene="rpoZ"
                     /locus_tag="OQM83_002232"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCS1520491.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10017..12125
                     /gene="spoT"
                     /locus_tag="OQM83_002233"
     CDS             10017..12125
                     /gene="spoT"
                     /locus_tag="OQM83_002233"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCS1520492.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12132..12821
                     /gene="trmH"
                     /locus_tag="OQM83_002234"
     CDS             12132..12821
                     /gene="trmH"
                     /locus_tag="OQM83_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCS1520493.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12827..14908
                     /gene="recG"
                     /locus_tag="OQM83_002235"
     CDS             12827..14908
                     /gene="recG"
                     /locus_tag="OQM83_002235"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCS1520494.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15077..16282)
                     /gene="gltS"
                     /locus_tag="OQM83_002236"
     CDS             complement(15077..16282)
                     /gene="gltS"
                     /locus_tag="OQM83_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCS1520495.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16562..17953
                     /gene="xanP"
                     /locus_tag="OQM83_002237"
     CDS             16562..17953
                     /gene="xanP"
                     /locus_tag="OQM83_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCS1520496.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTTLKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18074..19783
                     /locus_tag="OQM83_002238"
     CDS             18074..19783
                     /locus_tag="OQM83_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCS1520497.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19835..>21010)
                     /gene="yicI"
                     /locus_tag="OQM83_002239"
     CDS             complement(19835..>21010)
                     /gene="yicI"
                     /locus_tag="OQM83_002239"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCS1520498.1"
                     /translation="PGLAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDV
                     QWFDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDC
                     YANYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHG
                     SKSYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDP
                     ACDYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFL
                     SLPVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLK
                     ATRTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5085 a         5716 c         5388 g         4821 t
ORIGIN      
        1 agccataaag agagccgaaa ttctccagca tctcttttgc cagggttaat acatctttac
       61 cgcgcgttcc ggtacgcaga aatagcgcca gcagctcgac atccgttaag gcgctaatac
      121 caaacttcag cattttttcg cgcggcatca acagctgtgc attgtttttc accttcacct
      181 cctttgtggt gctcgcatcc tgtcatagcc gtttcgttaa atcgacggcc agttttcaat
      241 cctggaaagc gcctcgcaaa gtgaatctca ggtgaaggca cgacaacaaa agtattgtga
      301 taaaatcgcc aacttctggt gtcaccctac aggaaaaatc atcatgagcc tggccggtaa
      361 aaaaatcgtt ctcggcgtta gcggcggtat tgctgcctat aaaacccctg aactggtgcg
      421 tcgtttgcgc gatcgcgggg ccgacgtccg cgtagccatg accgaagcgg caaaagcctt
      481 tatcacccca cttagcttgc aggcggtttc tggttatccc gtttccgaca gtctgctgga
      541 cccggcagcc gaagccgcta tgggccatat tgagctgggt aaatgggctg atttagtgat
      601 tctcgcccct gccacggcag atttgattgc ccgtgttgct gccggaatgg cgaatgacct
      661 ggtatcgacg atttgtctgg ctacacctgc gcctgtagcc gtgctccccg ccatgaacca
      721 gcagatgtac cgtgccgctg ccacgcagca taatttagag gtgcttgctt cccgtggttt
      781 gctcatctgg gggccagaca gtggcagtca ggcttgtggt gatatcggtc ctgggcgaat
      841 gctcgatccg ttaaccattg tggatatggc ggtagcgcat ttttcgcccg tcaacgacct
      901 gaaacatctg aacattatga ttaccgccgg cccgacgcgt gaaccgctcg atccggtgcg
      961 ttatatctct aatcacagct ccggcaagat gggttttgct atcgccgccg ccgctgcccg
     1021 tcgtggcgcg aacgtcacgc tggtatcagg cccggtttcg ctaccgacgc caccgtttgt
     1081 taaacgtgtt gatgtgatga ccgcgctgga aatggaagtc gccgtgaatg cttctgtaca
     1141 gcagcaaaat atttttatcg gctgcgccgc cgtggcggat tatcgcgcag ctaccgtggc
     1201 cccagagaaa atcaaaaagc aggccacgca gggtgatgaa ttaacaataa aaatggttaa
     1261 aaaccccgat atcgtcgcag gcgttgccgc actaaaagac catcgaccct acgtcgttgg
     1321 atttgccgcc gaaacaaata atgtggaaga atacgcccgg caaaaacgta tccgtaaaaa
     1381 ccttgatctg atctgcgcga acgatgtttc ccagccaact caaggattta acagcgacaa
     1441 caacgcatta caccttttct ggcaggacgg agataaagtc ttaccgcttg agcgcaaaga
     1501 gctccttggc caattattac tcgacgagat cgtgacccgt tatgatgaaa aaaatcgacg
     1561 ttaagattct ggacccgcgc gttgggaagg aatttccgct cccgacttat gccacctctg
     1621 gctctgccgg acttgacctg cgtgcctgtc tcgacgacgc cgtagaactg gctccgggtg
     1681 actctacgct ggttccgacc gggctggcga ttcatattgc cgatccttca ctggcggcaa
     1741 tgatgctgcc gcgctccgga ttgggacata agcacggtat cgtgcttggt aacctggtag
     1801 gattgatcga ttctgactat cagggccagt tgatgatttc cgtgtggaac cgtggtcagg
     1861 acagcttcac cattcaacct ggcgaacgca tcgcccagat gatttttgtt ccggtagtac
     1921 aggctgaatt taatctggtg gaagatttcg acgccaccga ccgcggtgaa ggcggctttg
     1981 gtcactctgg tcgtcagtaa cacatacgca tccgaataac gtcataacat agccgcaaac
     2041 atttcgtttg cggtcatagc gtgggtgccg cctggcaagt gcttattttc aggggtattt
     2101 tgtaacatgg cagaaaaaca aactgcgaaa aggaaccgtc gcgaggaaat acttcagtct
     2161 ctggcgctga tgctggaatc cagcgatgga agccaacgta tcacgacggc aaaactggcc
     2221 gcctctgtcg gcgtttccga agcggcactg tatcgccact tccccagtaa gacccgcatg
     2281 ttcgatagtc tgattgagtt tatcgaagat agcctgatta ctcgcatcaa cctgattctg
     2341 aaagatgaga aagacaccac agcgcgcctg cgtctgattg tgttgctgct tctcggtttt
     2401 ggtgagcgta atcctggcct gacccgcatc ctcactggtc atgcgctaat gtttgaacag
     2461 gatcgcctgc aagggcgcat caaccagctg ttcgagcgta ttgaagcgca gctgcgccag
     2521 gtattgcgtg aaaagagaat gcgtgagggt gaaggttaca ccaccgatga aaccctgctg
     2581 gcaagccaga tcctggcctt ctgtgaaggt atgctgtcac gttttgtccg cagcgaattt
     2641 aaataccgcc cgacggatga ttttgacgcc cgctggccgc taattgccgc gcagttgcag
     2701 taatatgacg ccggatgact tttcatccgg cgagtttctt taaacgccaa actcttcgcg
     2761 ataggcctta accgccgcca gatgttccgc catttccggc ttctcttcca ggtaagcaat
     2821 caggtctttc agggtgatga tagagatcac tttgcagttg taatcacgct caacttcctg
     2881 aatcgccgaa atctcgctgc gcccccgttc ctgacgatcg agcgaaatca acacgccagc
     2941 aagcgtcgcg ccattggcct gaataatttc catcgactca cgaatcgccg ttccggcggt
     3001 gatcacatca tctaccagca ttacgcgtcc ttgtaacgcg ctaccaacca gattgccgcc
     3061 ttcaccgtgg ttttttgcct ctttgcggtt aaagcagtac ggcaggtcca ggtcatgatg
     3121 ctccgccagc gccacggcgg ttgtggtggc aatcgggatc cctttgtaag cagggccaaa
     3181 cagcagatcg aactcaatgc cggaatccac caatgcttca gcgtaaaaac ggcctaacag
     3241 tgccaggtcg cgcccggtat taaacagccc ggcgttgaag aaataggggc ttttacgccc
     3301 ggatttcagc gtaaactcgc caaactttaa cacctgcttg ctaagcgcaa attcaataaa
     3361 ctggcgctga tatggtttca tgccttcgct cctcatctta cttttctaca gacaaaaaaa
     3421 aggcgactca tcagtcgcct taaaaatcag tttgccagcg ccgccttctg cgtcgctaca
     3481 atggattcga ttccccctcg ggccagagcc aacaaggtga gtaattcttc gtgggtgaac
     3541 ggttcgcctt ctgccgtccc ctgcacttca atgatgcgcc cgtcttcggt catcactacg
     3601 ttcatgtcgg tctctgcggc agagtcttca acgtattcca gatcgcaaac cgcttcgccg
     3661 ttcacaatcc cgacagaaac cgcggctacc atccctttca tcggattggt tttcagctta
     3721 ccgttttcca ccagcttctg tagcgcatct gccagcgcca cgcaggcacc cgtaatcgaa
     3781 gcggtacgag tgccaccatc agcctgaagc acgtcgcagt ccagcgtaat ggtgaactca
     3841 cccagcgctt tcaaatctac tgccgcgcga agcgcacggg cgatcagacg ctggatttcc
     3901 attgtgcgtc caccctgctt acctttcgcc gcttcacgag cgttacgggt gtgggtagaa
     3961 cgcggcagca taccgtactc tgcggtgatc cagccctggc cctgaccttt caggaagcgc
     4021 ggcacgcctt cttcaataga ggcggtacac aacactttgg tatcgccaaa ttcgaccagc
     4081 accgagcctt ctgcatgttt tgtatagtta cgagtcaggg taacgggacg cacctgatta
     4141 ttgctacggc ctgctggacg catattgaaa tctccggctt gaaacaaatg tggctgcgca
     4201 ttatacggac ttccggaggt tattcctatc ctgacaaggc atcgatggct ataatccttc
     4261 cacctctcct tttataaaca ggaacgtcta tgatccgcag tatgaccgcc tacgcccggc
     4321 gtgaaatcaa gggtgaatgg gggagcgcaa cctgggaaat gcgctcggta aaccagcgtt
     4381 atctggaaac ttactttcgt ctgccggagc agttccgtag ccttgaacct gtcgttcgcg
     4441 agcgtattcg ttctcgcctg acgcgcggta aagtggaatg taccctgcgc tatgagccag
     4501 atgttagcgc gcaaggtgag ctgatcctca acgaaaaact ggctaaacag ctggtaactg
     4561 ccgcgaactg ggtaaaaatg cagagtgacg aaggggaaat caacccggtt gatattctgc
     4621 gctggccggg cgtaatggca gcccaggagc aggatctcga cgcgattgcc gctgaaattc
     4681 tcgcggcgtt ggatggtacc ctggacgact ttatcgtcgc acgtgaaacc gaaggtcagg
     4741 cgctgaaagc gttgatcgag cagcgtctgg aaggcgtcac cgccgaagtg gtcaaagtcc
     4801 gcgcccatat gccggaaatc ctgcaatggc agcgtgagcg tctggtcgcg aagctggaag
     4861 atgctcaagt gcaactggaa aacaaccgcc tggagcagga gttggttctg ctggcacaac
     4921 gaattgatgt tgccgaagaa ctggaccgcc tggaagcgca tgtcaaagag acctacaaca
     4981 ttctgaagaa aaaagaagcc gttggtcgtc gtctggactt tatgatgcaa gagttcaacc
     5041 gcgagtcgaa cactcttgcg tcgaaatcta tcaatgccga agtgacgaat tctgccatcg
     5101 agctgaaagt gttgattgag cagatgcgcg agcagattca gaatattgag taactcgtaa
     5161 cactaattct tacctttcag ccttcacaaa tgccgcccac tcaaacagag cggcattttt
     5221 cttccccgca acattcaatt ctgttttgcg tacctgctcc agattttgcg atgttttttt
     5281 gcgcagcgca gtgagaacgt gagatactca caactgtata taaatacagt tatagactta
     5341 ctttctttgc aatcgatatc acatggagtg ggcaatgaac gaacatcatc aaccttttga
     5401 agaaatacgg cattatggca ctgaagggca agaattttgg tcagcgagag aattggcacc
     5461 gttactggat tatcgggact ggcgcaattt tcaaaaggta ctggcccgcg cgactcaagc
     5521 gtgcgaagcc agcaatcaag ctgcatctga ccatttcgtt gaaaccacca aaatggttgt
     5581 attaggttca ggggctcaac gagaacttga agatgttcat ctctctcgct atgcctgtta
     5641 cctggtagta caaaacggcg accctgcgaa accggttatt gcggcagggc aaacttattt
     5701 tgctatccag acccgacggc aggagctggc tgacgatgaa gctttcaggc aacttcgtga
     5761 agacgaaaag cgtctttttc tgagaaatga gttgaaagaa cataacaaac aattagttga
     5821 ggcagcgcag caagctgggg tagcgacagc tatcgatttc gccatatttc agaatcatgg
     5881 ttaccggggg ctgtatggtg gattagatca gaaagccatc catcagcgga aggggctgaa
     5941 aaagagtcag aagatcctgg atcatatggg ttcaacagaa ctggcggcta atctctttcg
     6001 agctacccaa acagaagaaa aactcaagcg ggatggcgta aattcaaaac agcaagctaa
     6061 taccacacac tttgacgtgg gtagcaaggt gaggcaaacc attcaggaac ttggcggaac
     6121 catgcctgag gagttgccga ccccgcaggt cagcatcaat cagttggaaa acagtgtaaa
     6181 aattacagag aagaaatagc agaaactaaa aactcttttt cttaattgag atacccgatg
     6241 cgtaagccaa agtccaggtg cagtgaacac aatggctaaa cattgcacct ttcttttcca
     6301 cccctcaatt ttaacctatt ttttcttatg cattttctca gacaagaagt cagaagaatg
     6361 catctacact acagaaaata gcgatttcac ataactacaa gttatcaatt tcctctcacc
     6421 ttaaaaaatc tcaatcgtga caatacgcac aaatcgctac cctgccagac agatttttag
     6481 ggagagaacc atgctgttac acattttgta tttggttggc attactgccg aagccatgac
     6541 gggggcgctg gcggccggac gacggcgcat ggatacattt ggcgtaatta ttattgcgac
     6601 cgccaccgca attggcggag ggtcagtgcg cgatattctg ctgggccact atccgctcgg
     6661 ttgggtcaaa cacccggaat atgtgattat cgtcgctacc gccgcagtac tcaccactat
     6721 cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg gtgctcgatg cgctcggact
     6781 ggtggtcttt tctatcatcg gcgcacaggt tgcactggat atggggcacg gcccaattat
     6841 tgccgttgtc gcggcggtaa ccaccggcgt gtttggcggc gttttgcgcg atatgttctg
     6901 taaacgcatc ccactggtat tccagaaaga gttgtatgcc ggtgtctcat ttgcctccgc
     6961 cgtgctgtac atcgcgctgc aacactatgt ttctaaccat gatgtggtga ttatctccac
     7021 cctggtattc ggcttttttg cccgcttact ggcgctacgt cttaagctgg gattaccggt
     7081 cttttactac agccacgaag gtcactaagg ttcaaaacct gtgatctgct gggcagccag
     7141 ccaactgccc agcttcttga tttgcgcatt ttccttccat tcaataacct gtctggcgcg
     7201 cccagatcca gtccccggca gccgctgcca gaactgctcc gtgctaaata aaagttgcga
     7261 ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt aacggtattc ccattgccat
     7321 cacccagcga gtaaaaggct gcttacgagc cagattaaac tgatgccata actgcgcact
     7381 tttacttttc gcgatccccg gcgtgttctg taattgctct ggcgttaata aaagccagga
     7441 aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc cagccggcct caccaatgcc
     7501 atccagccca agaacctgtt ttgaccccag ccagactaag cgtgaaatga actgttcctg
     7561 acaaacatca gaagcaaagt agcaggtcaa cgagttaaag cggttttctg gtggtgtcgg
     7621 ttttgtacgt tctgcaccgc gccagaccac atcatcaatg cgaggaatac cctgaccggc
     7681 aaggctgacg aggatctgat caccaggcgc aatatcccac tcctgccagc gcctgacgga
     7741 accaatattc acccgctgga cttttttatc atccagcatg acaggtgcga gtgacgcaac
     7801 caccgatatt ttaccgctct tacccaccgc aaactgaatc gccttcactt cggcaacctg
     7861 agctacaggt tgatatttcc aggccaccaa ccattcagcc tggcctggta gccaatggcg
     7921 ggattcgggt tccttagccg ctcgcacaac cacgccatcg gtgacgaagg gtaatttcgc
     7981 tttccaccac tcattgcgta cgcgcgcaat ttcatcagca tttttaaccg cacgggtata
     8041 cctctgcgtt agagtaaaac ctgcggtagc cagctccttt aaacgatcgg tcattaactg
     8101 cggtccatcc ggccatgccc agacaaaaac agccagtgag tttagcgtgt cgctattccc
     8161 ctggcgcatc atcaggccag caacttttgc gcgggcattt attcccccca tttgttgttg
     8221 gatatgcccc tcgcgctgga gaaatatttc cccctgaagc gtactgttgg ctaaaggccc
     8281 gctaacggtt tgcggcacag cggaaattaa gctaactttc tgcgtccagt cctcgccttt
     8341 caggccatta ccgcgactga ttgctttgtt cagtttcccg tcccgataaa ccagggttac
     8401 cgcaacgcca tcaacttttg gctgcaccca aagatcgcta cgttctcgca tccacagact
     8461 taatgcgttt ttatccacca ttttgcgtac acccgtatgg gcaaccggat gcataaccgc
     8521 accatttaaa ggcggcatca tcacgtcgcg gggctcgctc ccaaagcagc gttgccactg
     8581 cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc acctcacttt tcccttcctt
     8641 ccagtagtca tcgtcccact gttttatttg ctgttgcagg cgggaaattt cttcctgtgc
     8701 tctggctggc gaccaggccg gacagaccgc ccacacagat gattgccagc acaagatact
     8761 tattaatatc gccatccata ctttcatcat cacctccgct gtagatagtc aggcagatat
     8821 acaacgtgat gaaagcaaag ccgagtggca aaaacggagt ctgcgaggac gcttcctgaa
     8881 aatcgtcttt attgcaatga atgacaggca aatgcggaag cagctacgca aaacgcaaca
     8941 actttgcgca aaaagtgtga gcaagggcta cgtcacatgg ccgcgccgtg tataataagc
     9001 tcgtatgtag gctttatttc gctaatcaca tacgaaagat actcatggct caaggcacgc
     9061 tttatattgt ttctgccccc agtggtgcgg gtaaatccag cctgattcag gctttattaa
     9121 aaacccaacc gttgtatgac acccaggttt ctgtttcaca caccacacgc caaccgcgtc
     9181 ctggtgaagt ccacggtgaa cattatttct ttgttaatca tgatgaattt aaagaaatga
     9241 ttagcagaga tgcgttcctc gaacacgcag aagtttttgg taattactat ggcacttcgc
     9301 gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt ttttctcgat atcgactggc
     9361 agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg gagtatcttt attttaccgc
     9421 cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg tcaggacagc gaagaggtca
     9481 ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca ttacgccgaa tatgattatc
     9541 tgattgtgaa tgatgacttc gataccgcgt tgaccgattt gaagaccatt attcgcgccg
     9601 aacgtctgcg catgagccgc caaaagcagc gtcatgacgc tttaatcagc aaattgttgg
     9661 cagactgaac ctgatttcag tatcatgccc agtcatttct tcacctgtgg agctttttaa
     9721 gtatggcacg cgtaactgtt caggacgctg tagagaaaat tggtaaccgt tttgacctgg
     9781 tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg cggaaaggat ccgctggtac
     9841 cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga aatcgaagaa ggtctgatca
     9901 acaaccagat cctcgacgtt cgcgaacgcc aggaacagca agagcaggaa gccgctgaat
     9961 tacaagccgt taccgctatt gctgaaggtc gtcgttaatc acaaagcggg tcgcccttgt
    10021 atctgtttga aagcctgaat caactgattc aaacctacct gccggaagac caaatcaagc
    10081 gtctgcggca ggcgtatctc gttgcacgtg atgctcacga ggggcaaaca cgttcaagcg
    10141 gtgaacccta catcacgcac ccggtagcgg ttgcctgcat tctggccgag atgaaactcg
    10201 actatgaaac gctgatggcg gcgctgctgc atgacgtgat tgaagataca cccgccacct
    10261 accaggatat ggaacagctt tttggtaaaa gcgtcgccga gctggtagag ggggtatcga
    10321 aacttgataa actcaagttc cgcgataaga aagaggcgca ggccgaaaac tttcgcaaga
    10381 tgattatggc gatggtgcag gatatccgcg tcatcctcat caaacttgcc gaccgtaccc
    10441 acaacatgcg cacgctgggc tcacttcgcc cggacaaacg tcgccgcatc gcccgtgaaa
    10501 ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat ccaccacatt aaaaccgaac
    10561 tcgaagagct gggttttgag gcgctgtatc ccaaccgtta tcgcgtaatc aaagaagtgg
    10621 tgaaagccgc gcgcggcaac cgtaaagaga tgatccagaa gattctttct gaaatcgaag
    10681 ggcgtttgca ggaagcggga ataccgtgcc gcgtcagtgg tcgcgaaaag catctttatt
    10741 cgatttactg caaaatggtg ctcaaagagc agcgttttca ctcaatcatg gacatctacg
    10801 ctttccgcgt gatcgtcaat gattccgaca cctgttatcg cgtgctcggg caaatgcata
    10861 gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat cgccattcca aaagcgaacg
    10921 gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg tgtgccggtt gaggtccaga
    10981 tccgtactga agatatggat cagatggcgg agatgggtgt tgccgcgcac tgggcttata
    11041 aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc ccagcgctgg atgcaaagcc
    11101 tgctggagct gcaacagagc gccggtagtt cgtttgaatt tatcgagagc gttaaatccg
    11161 atctcttccc ggatgagatt tacgttttca caccggaagg gcgaattgtc gagctgcctg
    11221 ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac cgatatcggt catgcctgcg
    11281 tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca gccgcttacc agcggtcaaa
    11341 ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc cgcgtggctg aactttgtcg
    11401 ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa aaacctcaag cgtgatgatt
    11461 ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg tggtagccgt aagctgaatg
    11521 aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat gaagctggca acgcttgacg
    11581 atctgctggc agaaatcgga cttggtaacg caatgagcgt ggtggtcgcg aaaaatctgc
    11641 aacatgggga cgcctccatt ccaccggcaa cccaaagcca cggacatctg cccattaaag
    11701 gtgccgatgg cgtgctgatc acctttgcga aatgctgccg ccctattcct ggcgacccga
    11761 ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca ccatgaatcc tgccgtaata
    11821 tccgtggcta ccagaaagag ccagagaagt ttatggctgt ggaatgggat aaagagacgg
    11881 cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa tcatcagggc gcgctggcaa
    11941 acctgacggc ggcaattaac accacgactt cgaatattca aagtttgaat acggaagaga
    12001 aagatggtcg cgtctacagc gcctttattc gtctgaccgc ccgtgaccgt gtgcatctgg
    12061 cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat taaagtcacc cgaaaccgaa
    12121 attaatgttt tatgaaccca acacgttatg cacgcatctg cgaaatgctc gccaggcggc
    12181 agcctgatct gaccgtctgc atggagcagg tccacaaacc tcataacgtt tctgcgatta
    12241 ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc cgtctggcct ggtagccgca
    12301 tgcgcaccat ggcttcggca gcggcgggta gtaacagctg ggtacaggtg aaaacacacc
    12361 gcaccattgg cgatgccgtc gctcatctga aaggccaggg tatgcagatt ctggcaaccc
    12421 atctttctga taacgctgtc gatttccgcg aaattgatta cactcgcccg acctgcattt
    12481 tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt ggccctggcg gatcaggaca
    12541 tcatcattcc gatgatcggc atggtgcagt cgctgaatgt ttccgttgcc tcagccctca
    12601 ttctttacga agcccagcgt cagcggcaaa atgcaggcat gtacctgcgt gaaaacagca
    12661 tgttgccgga agcagagcaa caacgcctgt tgtttgaagg cggctatccg gtgctggcga
    12721 aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa tcagcaaggc gagatcgaag
    12781 ctgatgccga ctggtgggct actatgcagg ctgcagggta agtgccatga aaggtcgcct
    12841 gttagatgct gtcccactca gttccctaac gggcgttggc gcagcactta gtaacaaact
    12901 ggcgaaaatc aacctgcata ccgtgcagga tctactctta caccttcccc tgcgctacga
    12961 agatcgcacc catctctacc ccatcggaga actactgccg ggcgtttatg ccacggtgga
    13021 aggcgaagtg ctgaactgca atatctcctt cggcggtcgg cggatgatga cctgccagat
    13081 cagcgacggt tccggcatcc tcaccatgcg ctttttcaat ttcagcgcgg caatgaaaaa
    13141 tagcctggcg acgggccgcc gtgtactggc ttatggcgaa gcaaagcgcg gtaaatatgg
    13201 tgcggagatg atccacccgg aataccgcgt gcagggcgat ctcagcacgc cagaattaca
    13261 ggaaacgctc acgccggttt atccaacaac ggaaggcgta aagcaggcca cgctgcgtaa
    13321 attaaccgac caggcgctgg atctgctcga cacctgcgcc attgaagaac tcctgccgcc
    13381 ggaactgtca caaggaatga tgacgctacc ggaagcgttg cgcactttgc accgcccgcc
    13441 accgacgcta cagcttagcg atctggaaac cgggcagcat ccggcgcaac gtcgtctgat
    13501 tctggaagaa ctgctggcgc acaacctcag catgttagcc ttacgtgccg gagcacagcg
    13561 ttttcatgcc cagccgctga gcgccaatga tgcgctgaaa aataaactcc tcgcagcctt
    13621 accgttcaag ccaacgggcg cacaggcacg cgtagtggcg gagatcgagc gcgatatggc
    13681 gctggatgtg ccgatgatgc gcctggtaca gggcgatgta ggttccggta aaacgctggt
    13741 cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa caggtggcat tgatggcacc
    13801 aaccgaatta ctcgccgaac agcacgccaa taacttccgc aactggtttg aaccgctcgg
    13861 tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa gcgcgactgt ctcagcagga
    13921 agccatcgcc agcggtcagg tgcagatgat tgttggtacg cacgccatct tccaggaaca
    13981 ggtgcagttt aacggtctgg cgctggtgat tatcgacgaa caacatcgct ttggcgtgca
    14041 tcagcgtctg gcgttatggg agaaaggcca gcagcaaggc ttccatccgc atcagttgat
    14101 catgaccgcc acgccgatcc cccgcacgct ggcaatgacg gcgtatgccg atctcgatac
    14161 ctcggtgata gatgaactgc cgccaggccg cacgccagtg actacggtcg ctattcctga
    14221 tactcgccgt accgacatca ttgaccgcgt gcgccacgcc tgcatgactg aaggtcgtca
    14281 ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta ctggaagcgc aggcggcgga
    14341 agctacctgg gaagagttga aactggcgct accagaattg aatgttggcc tggtacacgg
    14401 gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg tttaaacaag gtgagctaca
    14461 tctgctggtt gccactaccg ttattgaagt cggtgttgat gtgcctaacg ccagcctgat
    14521 gattatcgaa aaccctgagc gtctgggtct ggcgcagtta caccagctgc gcgggcgcgt
    14581 aggtcgtggc gcggtggctt ctcactgcgt gctactctac aaaacgccgc tttctaaaac
    14641 ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac ggttttgtga ttgcgcaaaa
    14701 agatctggag attcgcggcc ctggcgaatt gttaggcacg cgtcagacgg gtaatgctga
    14761 atttaaagtg gcggatttac tgcgcgatca ggcgatgatc ccggaagttc agcgcctggc
    14821 acgccatatt cacgaacgtt acccacaaca ggcaaaagcc ctgatagaac gctggatgcc
    14881 ggagacggaa cgttactcga atgcgtaaaa ggcggcagtg agaagaccgc catttcaggt
    14941 taccctacct tcctgccgga tgcgattcat caccctacaa attcaataaa ttatgaatca
    15001 atacgcaggc ttgataagcg aagcgtatca ggcatttgtc tttgttgcgg cggcacttct
    15061 accgccgctt catcggttaa ccggcaaaaa tcggcaacat caaatacaac ttaattacca
    15121 gcgcattgac gatatcgata aagaacgcac cgaccatcgg caccaccaaa aacgccatgt
    15181 gcgacgggcc aaagcgttca gtaatcgcct gcatgttggc gattgccgtt ggcgttgcac
    15241 cgaggccaaa accacagtga cctgcagcca gcactgccgc atcgtagttt ttgcccatca
    15301 tgcgccaggt aacgaagatg gcatacaacg ccatgaagat ggtctgtacc accagaatcg
    15361 ccagcatcgg cagcgccagc gaagccagct cccacagttt cagccccatc aacgccatcg
    15421 ccaggaacaa cgacaagctt acgttaccca gcacggatac cgcacgctca aagacgcggt
    15481 aaaagcccat tattgacaga ccgttgctca gaatcacgcc aacaaacagt acacagacga
    15541 aggtcggcag ttcaaaagca gtgccagcca aaagttgcgc aacaattttc cccaccgtca
    15601 ggcagatagc aatcagcgcg atagtttcaa tcagcaccaa cgaggtgatc atgcgtccca
    15661 catccggctt ttcaaacgcc gtcgggactt cctggtcatc cggaataccg ttcggcgtgg
    15721 tggagtgttt caccagatag cgcgccaccg gaccgccaat caagccgccc agcaccagac
    15781 cgaacgttgc acaggccatc gccacttccg tcgcattggt gaagccataa cgttcaatga
    15841 acaatttact ccacgcagcg cccgtaccgt gaccgccgga aagcgtaata gaaccggcca
    15901 acagccccat cagcggatca agccctaaca ggctagccat accaatgcca atggcatttt
    15961 gcatcaccaa cagaccaaca accacaatca agaagatgcc aaccacacgc ccaccggcac
    16021 gcaaactggc aatgttggcg ttcaggccaa tggtggcgaa gaaagccagc attaacggat
    16081 cgcgcaggga catatcaaag ttgacttccc agcccatgct ttttttcagt actagtagcg
    16141 ccagcgccac caacaaacca cccgcaacag gttccggtat ggtgtatttc ttcaaaaagg
    16201 agacggaatg gaccaactta cgcccgagca gcaacgtcag cgttgcggca acaagcgttg
    16261 ctaaagtatc gagatgaaac atagttactc ctttgtatcc gcatcactct ttcatactcg
    16321 tcatacttca tcgcgcagtg gcaacttcat gaactatgac gggtatcgag ttatgctttc
    16381 ctgggtcaaa ttctcagctg ttaatgaaca aatggcatgg attttaagca gaaaaatacc
    16441 aaaagttata taaaaaagtg catttatctc atttaaatat ttagatgagg atgtggcaaa
    16501 cgtttgcttt tctctttcgg tcagctaaaa tgcccgcttt gctaccacgg gattgttttc
    16561 gatgtctgtt tccaccctcg agtcagaaaa tgcgcaaccg gttgcgcaga ctcaaaacag
    16621 cgaactgatt taccgtcttg aagatcgtcc gccgcttcct caaaccctgt ttgccgcctg
    16681 tcagcatctg ctggcgatgt tcgttgcggt gatcacgcca gcgctattaa tctgccaggc
    16741 gctgggttta ccggcacaag acacgcaaca cattattagt atgtcgctgt ttgcctccgg
    16801 tgtggcatcg attattcaaa ttaaggcctg gggtccggtt ggctccgggc tgttgtctat
    16861 tcagggcacc agcttcaact ttgttgcccc gctgattatg ggcggtacca cgctgaaaac
    16921 cggtggtgct gatgttccta ccatgatggc ggctttgttc ggcacgttga tgctggcaag
    16981 ttgcaccgag atggtgatct cccgcgttct gcatctggcg cgccgcatta ttacgccgct
    17041 ggtttctggc gttgtggtga tgattatcgg cctgtcgcta attcaggtgg ggctgacctc
    17101 cattggcggc ggttacgcag ccatgagcga taacaccttc ggcgcaccga aaaatctgct
    17161 gctggcaggc gtggttttag ccttaattat cctgcttaac cgtcaacgta acccttactt
    17221 acgcgtggcc tcactggtaa ttgcgatggc ggccggatat gcgctggcgt ggtttatggg
    17281 catgttgcca gaaagcaacg aaccgatgac gcaagaactg attatggtgc caacgccgct
    17341 ctattacggt cttggcattg aatggagtct gctgctgccg ctgatgctgg tctttatgat
    17401 cacttcgctg gaaaccattg gcgatatcac ggcgacctct gacgtttccg aacagccagt
    17461 gtccggtccg ctgtacatga aacgcctgaa aggcggcgtg ctggcaaacg gcctgaactc
    17521 gtttgtttcg gcggtgttta acaccttccc gaactcctgc ttcgggcaaa acaacggagt
    17581 gatccagttg accggtgttg ccagccgcta tgtcggtttt gtcgtcgcgc tgatgttgat
    17641 cgtgctgggt ctgttcccgg cagtgagcgg ttttgtacaa cacattccag aaccggttct
    17701 gggcggcgca acgcttgtaa tgtttggcac catcgccgcc tccggtgtgc gtatcgtttc
    17761 tcgtgagccg ctgaaccgtc gggcgattct gattatcgcg ctgtcgctgg cggttggtct
    17821 gggcgtgtct cagcagccgc tgattttgca gtttgcccct gaatggctga aaaacctgct
    17881 ctcctccggg atcgccgcgg gcggtattac tgccatcgtg ctgaatctga ttttcccacc
    17941 agaaaaacag taattcactg gcggcggtaa gataattacc gccgcagtct cacctcttta
    18001 accattcccg ccttgaggat tgcgcgtaaa tcgtgcataa ctcccttatg tgcatttcac
    18061 gggatggaag accatgaaat ttattgggaa gctgcttctc tacattctca tcgctctgtt
    18121 agtggcgatc gctggcctct attttcttct gcaaacccgc tggggagcag aacatatcag
    18181 cgcatgggtt tccgagaata gcgactatca tctggccttc ggggcgatgg ttcaccgttt
    18241 ttccgcgcca tctcatatcg tgctggagaa tgtcacgttt ggtcgtgatg gccagcccgc
    18301 gaccctggtg gccaaaagtg tcgacattgc gctaagtagt cggcaactga ccgaaccacg
    18361 ccatgtcgat accatcctgc tggaaaacgg gacgctgaat ctcaccgacc agaccgcgcc
    18421 gctaccgttc aaagccgatc gtctgcaact gcgtgatatg gcgtttaata gcccgaatag
    18481 cgaatggaaa ctgagcgcgc agcgggtaaa tggcggcgtg gttccgtggt caccagaagc
    18541 cggtaaagtg ctgggtacga aggcgcagat tcagtttagt gccggatcgc tttcgctcaa
    18601 tgatgttcct gccaccaatg tactgattga aggcagtatt gataacgatc gcgttacgct
    18661 gactaacctg ggtgccgaca tcgcgcgcgg gacattaacc ggaaacgcgc agcgtaacgc
    18721 cgacggcagc tggcaagtgg aaaacctgcg catggcggat atccgtctac aaagcgaaaa
    18781 atcgctaacc gacttctttg cgccattacg ctctgtcccg tcgttgcaga ttggtcgcct
    18841 ggaagtgatc gatgctcgtt tgcaaggtcc ggactgggcg gtgaccgacc tcgatctcag
    18901 cttgcgcaac atgaccttca gtaaagatga ctggcagaca caagaaggca aactgtcgat
    18961 gaacgctagc gagttcattt atggttcgct gcatttattt gacccgatta taaacgcgga
    19021 attttctccg cagggcgtag cgctgcgcca gttcaccagc cgctgggaag ggggtatggt
    19081 cagaacgtca gggaactggc tgcgtgacgg gaaaacgttg atccttgatg atgcggcaat
    19141 tgccggactg gaatatacct tgccgaaaaa ctggcaacag ttgtggatgg aaacgacacc
    19201 cggttggtta aacagcctgc aactgaagag atttagcgcc agccgcaatc tgatcattga
    19261 tatcgaccct gacttcccgt ggcagctcac cgcgctcgat ggttacggtg ccaacctgac
    19321 gctggttacc gatcataaat ggggcgtctg gagtggctcg gcgaatctga atgccgccgc
    19381 cgcgacgttt aatcgtgttg atgtccgtcg tccgtcgctg gcgctaaccg ccaacagcag
    19441 cacggtgaat atcagcgaac tgagtgcatt tactgaaaaa ggcattctgg aagccaccgc
    19501 cagtgtttca caaacgccac aacgtcagac acatatcagc ctgaatggac gcggtgtgcc
    19561 ggtgaatatt ttgcaacagt ggggatggcc tgaattaccg ttgactggcg acggcaatat
    19621 tcagcttacc gccagtggcg atattcaggc caatgtcccg ttgaaaccta cggttagcgg
    19681 gcaattgcat gccgtgaacg ccgcaaagca gcaagtgact caaaccatga atgcgggcgt
    19741 cgtttccagc ggtgaagtta catcggcaga gctggtgcag taaacatcaa tctcgccggg
    19801 tacgactcag acgtacccgg cattcttcaa tagattacaa cgtaattgtc agcgcattcc
    19861 cttgaggcgt aaccaccagc ccctgctcac tttcagcctg cgaaccgcct tgcagaccat
    19921 ttactttcac aatattgcgc aagcacagtg tccagttcct cgcctcgccc gcaccagtca
    19981 cagtaatcgt gttaccagta cgtgttgctt ttagagtaaa gatcaccgaa ccgtcagcag
    20041 cgggcacttc acatacagct tcatgcccgt cttgcagatt aaagagatgg aatgccgtgc
    20101 cttcgtgcca cgcgtaatcg ggacgttgat cgttgttgcc cagcgccaat agggtgttat
    20161 cacgcacata aacgggcaga ctcaggaagc cgtgctgctg tttatgccag cgactaccgt
    20221 cgagctcatt gttgtgccac aggtgtgtcc agcgaccttc cggcaagtag aactgcacct
    20281 cgcccgcctc agtgaacacc ggagcaacca tcacgttgtc gcctagcatg tattgacggt
    20341 caaggtaatc acaagccgga tcgtccggga actccatcat catggcccgc atcatcggcg
    20401 taccccgcgc tttcgcacgc gcagcttcac gatacagata cggcatcatg cggcatttca
    20461 gttgcgtgaa gaagcgcacc acatcacagg actcatcatc gtaggcccac ggcacacgat
    20521 aagatttgct gccatgtaaa cgactatggc tggagagcaa accaaacgcg caccagcgtt
    20581 tgtaaacgtg tgccggcgcg gtattttcaa agccgccgat atcgtggctc cagaagccaa
    20641 aacctgaaag gccaatagac aaaccaccgc gcaggctttc cgccattgat tcgtagttag
    20701 cgtaacaatc gccaccccag tgtaccggga atttctgcgc accgacggag gccgagcggg
    20761 caaacaagac agcttcttcc tcaccaacgg tgtccttgag cacgttccac accagttcgt
    20821 tgtagatgta cgcataatgg ttatgcattt tctgcggatc ggaaccgtca aaccactgaa
    20881 catcagttgg gatacgttcg ccaaagtcgg tcttaaagca atcaacgccc atcgcgacca
    20941 gacctttcag tttgtcggcg taccatttgc aggcatccgg attggtaaag tcataaatcg
    21001 ccagacctgg
//
LOCUS       DAJVQG010000069        20491 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJVQG010000069 DAJVQG010000000
VERSION     DAJVQG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.069"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..4313)
                     /gene="mukB"
                     /locus_tag="OQM83_002240"
     CDS             complement(<1..4313)
                     /gene="mukB"
                     /locus_tag="OQM83_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCS1520499.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     EQEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     N"
     gene            complement(4313..5017)
                     /gene="mukE"
                     /locus_tag="OQM83_002241"
     CDS             complement(4313..5017)
                     /gene="mukE"
                     /locus_tag="OQM83_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCS1520500.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(4998..6320)
                     /gene="mukF"
                     /locus_tag="OQM83_002242"
     CDS             complement(4998..6320)
                     /gene="mukF"
                     /locus_tag="OQM83_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCS1520501.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6317..7102)
                     /gene="cmoM"
                     /locus_tag="OQM83_002243"
     CDS             complement(6317..7102)
                     /gene="cmoM"
                     /locus_tag="OQM83_002243"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCS1520502.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7238..8017
                     /gene="elyC"
                     /locus_tag="OQM83_002244"
     CDS             7238..8017
                     /gene="elyC"
                     /locus_tag="OQM83_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCS1520503.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(7994..8887)
                     /locus_tag="OQM83_002245"
     CDS             complement(7994..8887)
                     /locus_tag="OQM83_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCS1520504.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9041..9787)
                     /gene="kdsB"
                     /locus_tag="OQM83_002246"
     CDS             complement(9041..9787)
                     /gene="kdsB"
                     /locus_tag="OQM83_002246"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCS1520505.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9784..9966)
                     /gene="ycaR"
                     /locus_tag="OQM83_002247"
     CDS             complement(9784..9966)
                     /gene="ycaR"
                     /locus_tag="OQM83_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCS1520506.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10018..11250)
                     /locus_tag="OQM83_002248"
     CDS             complement(10018..11250)
                     /locus_tag="OQM83_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCS1520507.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11287..12273)
                     /gene="lpxK"
                     /locus_tag="OQM83_002249"
     CDS             complement(11287..12273)
                     /gene="lpxK"
                     /locus_tag="OQM83_002249"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCS1520508.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12270..14018)
                     /gene="msbA"
                     /locus_tag="OQM83_002250"
     CDS             complement(12270..14018)
                     /gene="msbA"
                     /locus_tag="OQM83_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCS1520509.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14055..16319)
                     /locus_tag="OQM83_002251"
     CDS             complement(14055..16319)
                     /locus_tag="OQM83_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HCS1520510.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASLIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQIAVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16526..16810)
                     /gene="ihfB"
                     /locus_tag="OQM83_002252"
     CDS             complement(16526..16810)
                     /gene="ihfB"
                     /locus_tag="OQM83_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCS1520511.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(16970..18643)
                     /gene="rpsA"
                     /locus_tag="OQM83_002253"
     CDS             complement(16970..18643)
                     /gene="rpsA"
                     /locus_tag="OQM83_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCS1520512.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18754..19437)
                     /gene="cmk"
                     /locus_tag="OQM83_002254"
     CDS             complement(18754..19437)
                     /gene="cmk"
                     /locus_tag="OQM83_002254"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCS1520513.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPASDALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19610..20374)
                     /gene="ycaL"
                     /locus_tag="OQM83_002255"
     CDS             complement(19610..20374)
                     /gene="ycaL"
                     /locus_tag="OQM83_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCS1520514.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4923 a         5681 c         4983 g         4904 t
ORIGIN      
        1 atattttccg gcgctgcgat gatgagctgc atttgcaaac gttcacacaa ttcaaacagc
       61 gtggcgatag aacgagcatc cagtcgcgct gcttcatcga ggaacagcag gcggcaagga
      121 gagatatctt taccgcgcag gcggcgagat tcatcttccc agctttgtac caccatcacc
      181 agaatcgaca taccggtacc aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc
      241 aaccagccat cggaaccacg gttaacctca acttccattt ccagatagtt gcggtaatcc
      301 agcagttctt cgccaatggt ctgcggcgtg cgctgcccca tatcaatctg cggattaaga
      361 cgctgataca atttcgccag cgcttccgag aaggtcagac ggttgctgtt aaacagatcc
      421 tgatgctgct cgtgctgttc agagagcaca tccagcagca tggcgtgcgt ttcacgcacg
      481 ttcacgttca gacgtacgct gttcacctga ccaaacgata cgttctgcaa cccctggttg
      541 agcatacgga tacggttctg ttcgcgctga atggttttgc gaatgatgtt cgccacgctg
      601 cgggaactga tcgccagttt ctgttcgcgg gaggttaact cctcggtcag tcggctaagt
      661 tcgatttcca tctgctcgat ggcttccacc ggatcatcgg tacgaataat atcctgacga
      721 atacgttcac gcagatgctg ataaaccgcc acgaagaact gaattttacg ttccggacgt
      781 tttggatctt ccgacatgcg cagcacgtcg cgcagatgtt cgttatccgc caccgccaga
      841 cgcagcgcac ccaatgcctt atccgacata gagcgcaaat catcagcgga gagataagcc
      901 agctcgcgac ggtgtaagcg ccgttcaacg acgttatctt tcaccatgcg catcactgca
      961 caccagcccg ctttggcggt cactacctgc tcgcgcatct caaagtaatc ccgctccagc
     1021 ttgcgcagtt tgcgggtcag attgtccatc tccgcttcgc agaaagtcag tgctttttcc
     1081 agctggttgc ggcgggaccg gttattactc agttgcgcat gcagctcgtc acggcgaatt
     1141 cgcgcccgct cttctgcccc gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc
     1201 agatcgttga gtagctcttt tttggtgtcg taagaacttt tcagcgaagc cagcacctgg
     1261 ttgtactgac tcaactgcgc tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc
     1321 gcttccgcct gttccagacg ttcacgcagc ttttcgttga gatcgctatt accgctcagc
     1381 atttctgccg agtcagaata gctaaagtgc gcacgacgct gcaccacttc cgtcagggca
     1441 aacgcctgct gacgggcatc gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc
     1501 tgttcgaact gttccgggtc gctctgcaat accgaaacga tcggttccag tttcgccagt
     1561 tgattgccaa actgctgaac aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg
     1621 atttcatcga cgcgatccgc caggctgtca tcagccaaca ggttgagacg cggcagaatg
     1681 cggttcagcg ccgtaacgcc ttctttcgcc tgctcaaact gaatacgctg ctgctggtta
     1741 tcattttcat gattactcaa cgcccgctcc agttcgacgc gacggctgtt cagttgacgg
     1801 atttctgctt ccgggtcaga ctcaaacgcc accgccagat gactgccgat aaagcggctg
     1861 aacgcctgat gcagacgctg ggttttctgt acatcaaagg agagcgtggc gaagcgttcg
     1921 gaaagcactt cacgctcggc atggaggctt tcaatacggc tttcacgcgc agcacgacca
     1981 aacagcggca cttccgggaa acgtgaataa cgccactgac gatcggcgat tttcaccact
     2041 accgcttttt ccagctcatc aacgctgaac acgctgtcat cgaatgactg cggatcccct
     2101 tcgatcaaat agagatcttc cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt
     2161 gacagatctg gcaccacgat ggcgtggcgt gacgggccat acagcgctga gaagtacggc
     2221 gcatcttcca ggctaacgtc gtcataaatt tctgacagca gcacgccgcc aaaacgctcc
     2281 gccagcgcgt tcagacgctg atcttcagag ccgccaggct ggcttaaacg ttcgatctct
     2341 tcatcgacag cgtttttgcg cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca
     2401 cgctccagca actgttgcag atattcggtg acgtcctggc tggaggtaaa ctcttcaccg
     2461 cactgctcgc tcaactggtt gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc
     2521 atcaaactct gaatgcgaga ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct
     2581 tcacgggcgt tagacacgct atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc
     2641 agagcttcca gttcatcaat atcaaaattc ttgccctgac gtttgcagaa gtctgccagc
     2701 aggcgttcgg cttcttgctg ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc
     2761 aacggttgaa cctgctctgc caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg
     2821 gcgacatccc aagcctcgtt acgcgccagc gggccgttga ttgccaccac cagctgataa
     2881 gcctgctcaa actggctgtg cgcggtttgc gccatgctca ttttctgctc aagagagagc
     2941 attttttcag tcgcttcctg ctctttcgcc tggaaggttt ccagccattc ggcggcgctg
     3001 tcggcggtta agtccggcag atggcacagt tctttggcgc gattaagcgc agcaatcgcc
     3061 tggttatact ggatcgcgcg cgtttgctgg acgtccagcg cctgctggta gtcagcaagc
     3121 tggcttttca gctcgtccac ttccagttcg gcagcttccg cgcgagcctc gttctcttcc
     3181 tggcgttcga tggcttctgc caccacttcg ttttgctctt ccagacggat ctgcagctca
     3241 tcgagatccg cttcgtagcg ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg
     3301 ttcaggtgat cgctggccgc ctgataatcc gcttccagat cgccttcggc accgttgtgc
     3361 tctgccagct cacgcgccat atcgacgtgt ttgtactgct cagccgccag ttgctgacgc
     3421 gaagtatgta actcgcgacg aaactccagg gctttgtcga gatggacacg gcgctcgttg
     3481 gcgtgacgca tgtagtccgc cgccacgtag ttggtagctt cgctgatcag atgcttaaac
     3541 aggtcacggt ccgactgggt gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc
     3601 gccgcttcca tgtcctggaa cgctttacgc acgccgctgt tttccggcaa caggtagtcg
     3661 cgcagagaac gggtaatggc actggagatc ccgccataca gcgaagcttc gatcagacga
     3721 tagaatttgc tacggtcaga tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac
     3781 atcagcgagt ggtaatcggt aatggagttg aactgtttaa actgcacgcc ttccatcgcc
     3841 tcgagcttgt ctttcagctc gttaagcggc agcacgcgcg cctggcgttc gttcagggtt
     3901 tcggtcacca gctgtgtcgg ctgcaccgac atcggcagtc cctgaatggc aaatggtttg
     3961 atatccactt tacgatcgcg tccggcaacc tgttgcagac gcacaccgac caccacgcgc
     4021 tggtggcgcg agttaatggt gtcgagcatc gaataacaga cacccgcttt cagcttaccg
     4081 tgcagacctt tatcgcgcga accgctggtg gccccggctt ccgtagtgtt acggaaatgc
     4141 agcagggtca ggtcggggat cagcgccgta acgaacgccg ccatggtggt ggatttaccc
     4201 gccccgttac cgccagaaag cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa
     4261 aagccgttcc agttaatcag cgtcagtgag cgaaatttac cgcgttcaat cattattctt
     4321 cctctccgct atctggctga ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa
     4381 tcggcattgc ttcgccatca cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag
     4441 cacgcacatc ggctccgaag cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt
     4501 ggcccataaa ccacaccatg cctaaacgac gcagacggtt gagcgaagaa cgtactttct
     4561 cctgcaactt ctgacggtca acgtctgaac cggttgaacg gttgttcacc agtttcagca
     4621 gttttgcttc atcggccagg gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc
     4681 cctcattcgc cagccgttcc gggctgagat agagataaca gaggattttc ccgaccatca
     4741 tatccagttc cgacaagacg gaacgcggga taagagtggt ggaacgtggg cgtaaataga
     4801 agaacccttc cggtgcgcga ataagctcaa cgttataacg cgcgtaaaac tcttccagat
     4861 attcctgaaa atccatcagg aatgcatgat tatccagttc gtcgaggcca atatggcggc
     4921 ctgaacgtaa ggcgctgtcc agcgccggaa ataacggatt cgccagcgcc tgcgccagct
     4981 taaccgacat cacttgttca atatttgtcg atgacatgcg cctgtacctt ggctccgtaa
     5041 tcattaatcg gctgccattt cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga
     5101 cgtaccgcct gatcaataac aatacgcgca acgtcaaagt gacgtgcacg cggatactgt
     5161 gacagatatt cgcgtaccac cagaccaaga tccagcggca cttgtctggt tttgtacacg
     5221 gcaagttgtt cttcgatgat cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat
     5281 tccagatcct caggaagttc cccagtcact tcttcatcgc gcagtgccat ctcttcgtca
     5341 cgcatatcca gcagacgatc ggcattggca taagttagcg cccacggctc atcaaaatag
     5401 gtttgtaccg actgacgtaa ccgctgagca aagacgcggt ttttatccat atcgatcgcg
     5461 gtacgaataa atttgtgtac gtggcggtcg tagccaatcc acaagtcgat ggattgctgg
     5521 ccccaactga taatacgatc gagtttgctc tgcagatcga acaccagacg atcgacgaaa
     5581 tgcagatcgt catgggtcat cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg
     5641 tcgcctgccg cttccagcgt atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc
     5701 aacaattcac agctggaaat cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg
     5761 tccttcacct gctgctgctg ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa
     5821 atttctgcta ccgaatattt cagtggcgca tagacattac ggtgccagtg aaattcatca
     5881 ccgccctctt cggcggcatc tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc
     5941 atagaaagac gcagcgtaga aaactcgcgc tgacggatgt agtagtcagt aatgccgatg
     6001 ccgagcggcg tcagacggta aattgcgttc ccttccgcct gctcgctggt aaagcggttc
     6061 agcagacgtt gacgcaccat gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg
     6121 ctggtttgct caaacgcatc actcacatgg cggaatgcat ccaccagctc gccttcactc
     6181 atctcaccat ccagacgctc gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg
     6241 tctaccggca gcgagatgga gaagtcattt tttctggccc aggcaaccag ttcggggact
     6301 gtctgggaaa attcactcat actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa
     6361 tataacgccc cagggtaata tacggttcct gacggcaata acgcgtttct aattcaagta
     6421 atgcttcata gcagtcgcgc tgctggtgct tctcgcgcag ataatcatga aacacgcgaa
     6481 cgcctgtctt acccataatt tgccaaccag cttcttccag ccacagataa acctgcgccg
     6541 ggtcgcgtgg ataatctggc gaaagcgtcc gttttttctt tttcggcatt cccgcctgca
     6601 cgtaatcaaa attcccggcg accatgttat gcatcaacaa accatgcgca ttgtagaaca
     6661 ttaacgacaa cacgccgcct ggacgcaaca ctgaccacag ggtctgcaat acactgcggg
     6721 gatcggccac ccactcgagc accgcatgga acaatatcag atcaacgggc gtttccaaat
     6781 gcgaagcaac atcctgagcg gcgcaatgta taaattgcat gttgtcgctc acaccttttg
     6841 cttctgccgc ctgttttgcg cggtcgatca tctgcgcaga aagatcgcat aaaatgacct
     6901 gatgcccgcg ctcggccatt ttgattgcgg tctgcccttc tccaccgccc gcatccagca
     6961 cacgcagttt ttgcggaccc atttccgcca gcacgcgatc gagatcctgc cacagaatag
     7021 cctgtcgaag ctgccctttg gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat
     7081 catcaaaatt gcgatcctgc attgctttct ccaccggttg gctaagacgt tttctgataa
     7141 agacgctatt ttgtcacagc cacggcgaga atgaacccgt ctggtgtaat atcgctggcg
     7201 atcccttgct atatggttaa aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt
     7261 gattggcaat atgttgctgc cacttccgct gatgttgtta attattggcg ctggcctggc
     7321 gctgctgtgg tttagccgtt ttcaaaaaac cgcgaagatt tttattagca ttgggtggct
     7381 ggcactcttg ttattgagtt tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag
     7441 cacctatccg acatggaata attcgcaaaa agtggactat atcgtggtgc tcggaggtgg
     7501 ttatacctgg aacccgcagt gggcaccgag ctctaattta atcaataaca gtctgccacg
     7561 cctgaatgaa ggtattcgct tatggcgcga aaatccggga tcaaaactga tcttcacggg
     7621 aggcgtagca aaaaccaata cggtgagtac agcggaagta ggtgccagag ttgcgcaatc
     7681 gctgggcgtg ccgcgcgagc aaattatcac cctggatttg ccaaaagata ccgaagaaga
     7741 agctgcggca gtgaaacagg cgattggtga cgcccctttc ctgctggtga cctccgcatc
     7801 acatctgccg cgggcgatga tcttttttca gcaggaagag ttaaatccgc taccagcccc
     7861 ggcgaatcag ttggccatcg actcgccgct taatccgtgg gaacgggcaa tcccttctcc
     7921 ggtctggctg atgcatagcg atcgtgttgg ctatgaaacg cttggccgca tctggcagtg
     7981 gctgaaaggt ccgtcaggcg agccacggca agagtgattt tgatgccaga tcgaagttgc
     8041 gccgactaaa tcgtccggta ttaaccagtt gcgcaacttc atcccataac acatacaacc
     8101 aacgccgcca gatgaacgac tccgccactg gcgcgcgttg caggtaactc cagagcaaat
     8161 cttccgccag agaattatcc attagtcgga acagttcgta ttctcgcggt gcccaaagca
     8221 ttagtcccgg cccgaccatc gccagtaact gatcgctgcg cgaatctttc agcatgctgc
     8281 gtaaacagaa gttaccgtga atcaacacac aattgtcgtt aaagccttca aataatgccg
     8341 ggagacattc gcgagtgcga aacaggatcc gcttatcctg catcgtcaga ccggtgttat
     8401 tgaactgatt gagcgtggtc catagcactt caacatgttg ccggtaccat gagggccaga
     8461 aattttcctg agtattgtcg accgcgccga cgcaaccgcg actgtcctga cggtgccagg
     8521 ccagtaaggc ttcaacgatt tggtctttga gctgttccca gcgttctggt gttcgggctg
     8581 gtgcctccac cgaaacacca cgcatccgct ccagtagcag gacatcaggg ccggggtgtt
     8641 cttcatgcgt catcacacca taaatagtcg gcatacggac agtcccactt cttgccagca
     8701 tggtggtttt ccatgccagt tgtcgggctt ttccgggcgt actaaagctt cttgccatta
     8761 acggcattgg gtttccctgg ctgtcataca aagcccacaa cgccgtatcc gctttttcat
     8821 tgacgcactc aatacgactg agtttttcgc ccagtaaatg gcttaattcg gcacgcagct
     8881 gttccattca gattaccctc ataaaagcta ctgctttcat aatgagcgcc aatataaaag
     8941 atgtcacctg aatgagatca attgttgaaa agaaaaattg aggaaaatcg taacggtacg
     9001 acactcctcc caaaattggc tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa
     9061 cgcgctcaag atcttcaggg gtatccacac ctgtgccagg aacttcctga gcaacagcaa
     9121 catggatttt ttcgccgtac cacagaacac gaagctgctc taacatttcg atgtgttcta
     9181 acggacttgc ctgccagttg acgtaacgac ggataaagcc tgcacggtag ccataaatac
     9241 caagatgacg caggaagtta tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat
     9301 cccaaggaat ggtggcgcgt gagaagtaca gtgcataccc ttcagcgtcg agaaccactt
     9361 tcaccgcatt cgggttaaac gcttcttccg cattgtggat tggcaccgcc agagtcgcca
     9421 tacccacctg acgctgagcg aggttatcag caacctgacg aatgattgtc gcagggatca
     9481 tcggttcatc accctgcaca ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga
     9541 caacttccgc cagacgttct gttcctgact gatgatcggc gcgcgtcata catacttcac
     9601 cgccagcggc ttcaacggcg cgggcaacat cctcatgatc ggttgccacg atgatgcgct
     9661 cggcacctga ttcacgcgcg cgttcaagaa catgaacaat catgggtttg ccgttaatat
     9721 caaccaatgg tttaccgggc agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa
     9781 aactcatgat ttactctcat cagcagtcag cacgcgggct tccgtttcca gcaacactgg
     9841 aatgccatcg cggagcggga aagccaggtt atccagtttg caaataagct cttgtttttc
     9901 ctggttatac caaagttttc cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg
     9961 atccatagtt cctccagatg gatcgggtta tgaatgcata aatcttatca taatcattta
    10021 tgcgacgggg tctatttccc agccagtgcg gcaatcagta aagagacctt gcgggcagcg
    10081 tcctaatgtc acccgcgttg cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa
    10141 ccctttttgc agcattgtcg ttggtttaat gccttcctgt aaccacagag agataacttc
    10201 aaggatgcct gtctggcgat gcattttggc atccattcgc ccaactaatt gcccacgatg
    10261 taataatggc agaacaaaat agccatactg gcgtttcggc gctggggtat agcactccag
    10321 ccggtagcta aaatcaaaaa gctgctctgc gcgtttgcga tcccagacaa caggatcaaa
    10381 aggcgaaagt accgcgctgt gcgttgcagt gagctttccg gccagcgcac gctcgagtag
    10441 cggcagcaaa tcagcatgca gccaaagatt gcccaatttt tcaacatgca cagcaatgat
    10501 ttgctgctgt tcagccctcg cttcgcgcca cgccgccagt gccggacgtt tcagccgata
    10561 gtaatctgcc agccactgtt cgcggaatat tcccaagcta cgcgcactgt tatccagcat
    10621 gatgatttct gcttctgttt gcgaaacgag atcgcgctca tcatcccagt caggcatgac
    10681 acggtgggtt aaatcataaa cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc
    10741 ggcagtaaat aaaccttcca gatgccgttt atgcggcttc cattcccacc agccgcttgc
    10801 acctttacga ggatgctcaa aatcggctga acgtaccggc cccttatcat gaatatgctg
    10861 aattaactgt gcaatttccg cctcatgttc ctgcatccag gcgtctttgt atttccagcc
    10921 cattttttca ggtgccagca tgcggtggcg aataagacga aagtcgctac gcggcataaa
    10981 gcaggcttca tgcgcccagt attccattaa ttcgccacgc gccagagact catccagcca
    11041 ctgggcagga taatttccca gacgactgaa aagcaccaga tatggactac gggcaacaat
    11101 attgatggta tcgatttgca gcaaggacat gcgggagatc gttgccggaa tatcctccaa
    11161 cgacgctcga cggcggggtt tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc
    11221 atcagcaagg gagaggtgcg gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg
    11281 gcgtaactag ttgccagaag ccagcgaggt tagttgcgta agcagtttcg ctggttcatc
    11341 acctgaaagc tgtgcgtcta caggcaaata ccaccaattt tcttctgcaa aggcccggca
    11401 tttcaccgca tctttttcag tcattaccag cgtttgcccg gcgcttacca acgcactgac
    11461 atccgcatgg ttcaaagact gatgatcggc cagcggtaca catttttccg gttgtacacc
    11521 acacatcttc agcgtggcaa aaaagcgcgg cggatgccca atccccgcca tcgccactac
    11581 atgttcaagc tgagcaacgt cacaacgcgt accggtacgt aaattcaccg cctgacccgg
    11641 cagcagatgc atggggattt caccgctgcg agggacaccg ccgttgacga ttaccgcatc
    11701 aaccgacttt aagcgccccg ctcgctcacg cattggcccc gccggcaacc accagccatt
    11761 gccaaagcga cgcacaccat caataacgac aatttccaca tcacgcgcca gacggtaatg
    11821 ctgtaaaccg tcgtcggtta cgatgatctg cacatcaggg tgttgcgcca gaatggcttt
    11881 taccgcatca gaacgaacgg gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa
    11941 cacaggttca tcacccgcct gtgctgttgt ggtatctgcc gacaataaca gcggataaga
    12001 ttcagcctta ccaccatatc cccgcgatac gacccccacg cgaataccgc gctgttgcaa
    12061 ctgttccacc agccagacaa cgaccggggt ttttccgttg ccgcctgcgg tgagattacc
    12121 aaccacgaca accggtacgg gggcacgcca ggcgcgcttc agttttagtt tatagcaaag
    12181 acggatcgcg ccactcacca ggccatacaa ccaggagagt ggcagcaata gccgccacaa
    12241 aggggattca ccagaccaga ttttttcgat cattggccaa actgcatttt gtgaagttgc
    12301 gcgtaaacgc cgcggtgctc aagcaaatcg ttatgcgtac cgcgttccac aatgacacca
    12361 tcctcaacga ccacgatttc gtctgccttt tcaatggtag acaagcggtg ggcaatcacc
    12421 agagaggtac ggtttttctg caactcatcc agtgccgcct gaatcgcacg ttcggattcg
    12481 gtatccaacg ccgaggtagc ttcgtccaga atcagaatcg ggctatcacg caacaaggct
    12541 cgagcgatag caatacgctg acgctgaccg ccagagagca gcacgccgtt ttcaccaatc
    12601 actgtatcga gaccgttatc catcttattg atgaagtcta tggcgtaggc catacgcgcc
    12661 gcttcttcaa tttgctcacg gctgtactgt tcagtccgtg cgtaagcaat gttgttagca
    12721 accgtatcgt taaacagatg gacattctgc gacaccagag caacctggtt acgtaacgac
    12781 gccagggtat actcgcgcag atcgtgacca tccatcagga tttcgccttc atcaatatcg
    12841 taaaaacgcg tgatcaggct ggcgatggtt gatttacctg aaccagagcg tccaaccaga
    12901 gcgaccgttt tccctgccgg aattttcagg ttgatgttac gcaatgcggg cacgtcacgt
    12961 cccggataag taaaggtgac attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg
    13021 cgcttacctt cgtctttctc ctgctcactg tccagaatgg taaacagcgt ctgacaagcc
    13081 gccataccgc gctggaactg ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt
    13141 gcaatcattg aagagaaaac aacggtaatc gtaccggcag tcaggttatc cataacactt
    13201 gggaagctcg ccgcatacag aacaaacgcc agcgccaaag aggcgattag ctgaatgatc
    13261 ggatcagaga tggaagaggc tgaaaccatt ttcatcccct gaagacgcat tcggttactg
    13321 actttatcga agcgtttcgt ttccacttcc tgaccaccga aaatcaatac ttctttgtgg
    13381 cccttcagca tttgttctgc gctggtggtc acctgcccca tggtgttctg catgttttta
    13441 ctgatgttgc gaaaacgctt cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc
    13501 acaatcaaaa tgatcgacag ttgccaactg taatagaaca tcatgatgaa caggccgatg
    13561 attgacgcac cttcacgcac aaccgttatc agtgcgccgg aagaagaaga agcaacctgt
    13621 tcggaatcgt aggtgatacg tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa
    13681 accggcattc ccatcatgtg accaaacagg cggcgacgca tggtcattac cacctttcct
    13741 gatacccagg agatacagta gctggagaca tagctggtga taccacgtaa aatcatcagc
    13801 ccgatcacca ccagcggcat ccacaccagc acggagcgat ctgttttacc aaagccatca
    13861 tcaagaagtg gcttaaggag cgataacatg aaggtatcgc tggctgcgtt gaggattaac
    13921 gctacgcccg ccacgatcag acccgcttta aaaggcgcaa tggttggcca cagtcggcgg
    13981 aacgtctgcc acgtagagag atctttgtcg ttatgcattc aaaaaaccag catttgttga
    14041 aatagccgca tattctaccc gttatccact ggcacgccaa accactgatg ataccaacga
    14101 ggtaaaattt gctccctgag gctgctaatc cgccagcctt gcgctgaaaa attgaccgct
    14161 atttgcccct gatgaggagt atcaagccac ttataaccct gttgttgata gcgatgttta
    14221 actttgcttg agggcaatcg ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt
    14281 ccattcactc gctgaattaa tggcaacgat gatgaggtat tactgccatg gtgaggtacc
    14341 tgaagcaatg ttgtctgcac ttgctgccag taacggctta gcatcttttg ttcagcgggg
    14401 acttcaatat caccggttag aagaatgcta ttcgtcccgt catcaacctt aaccacacag
    14461 gaatggttat ttcctttatc gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat
    14521 ccttgccatt gccacgcttc gccacgcaca cagggctgat gatgttccca gtttaacgga
    14581 cttctgatcc ataacatcgg ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc
    14641 agatgttcat gactcagaat aacgccttcc ggttcaagat tatgccagtg gagccagggg
    14701 ataatcagtt gttgcccact atccccttcg ggccaggcca gtcctgtgtc atagagaatc
    14761 gctttgccgt ttctggctat caccattgcc agcccttgcc cgacatcaag catgtacacc
    14821 tgccactcgt caggtcgagg tttttgccac agcggccagc acatcagcaa gcctacagcc
    14881 acacacattg ctggcaacgt tcgccaggcg tttaatcgcc ataccaccag taagaaccat
    14941 ggggaaaatg atagccattg ccaacgttca gcaatgttga tccacccttc tggcaaactc
    15001 tttaaccccc agaaaagtaa agccaaagac cggtcggcaa gaaaccataa cccttgctcc
    15061 aggattaacg gcccgcttaa atgcacaacc atcgcggcga ggatcaacgg aaccgtgata
    15121 aatgtcacca agggaattgc taatagattt gcaataaacg aggtcagact aatgccatga
    15181 aatatgacga tttgcacggg cataagcaga agtgtgatac ccagttgcag atggatgagg
    15241 gaaacaactg cacgcaatac cggcggcagt tgccactcag gacagggaaa ccactgatac
    15301 caaaaaatca gtgccgcgac cgcagcggca gagagccata aactttgcga gagaatggca
    15361 acaggatcca tcagcaaaat tgccgccaga caacatatcc atacatccca gccactccac
    15421 tgtcgcccac ttaacttaag cattccccac gtagcaagcg ccaccacggt acgcaatgca
    15481 ggaggttgca ttcccgtcaa ccaggcataa aaagcagcac agcagatccc accaattaat
    15541 ggtatttgcc agtggatcca gcgcccaggc agaaaaattt gtccactgcg aatgagtccg
    15601 gcagccagca acaccgcaaa agcgatgtgc aatcccgata tcgccattaa atgcgccgtt
    15661 ccagtatcgc gcatgatatt tttgatttct ttggggacgg ataaccgttc ccccatacct
    15721 aaaccaagaa taaccgcatt ccacggatag ggttgcagcg ttgtttgtag tgacgccaga
    15781 tactgtgcac gcaggctaca attcggttca ataagacttg cctgcagaaa gcggccggtg
    15841 agcggctgat gctgggcaat ggcataacgc tggctatcaa agccgccatc attaagttga
    15901 ccatgaacgg cacgaacttt gagtttcatt gaccacagtt gtccggcaca aaccgcttgc
    15961 ggaagatatt cgccatacag cacaaggcct ggcgcaggga atatacgtcg accttgtaga
    16021 taagtaattt gaccgtaatg agtggtcatg ccgtcagtag cagtgatctc aacaattgca
    16081 tcctgcgtcg cgccagttaa ggtttctcct gcccacaaaa tttgctttgc tgataatatg
    16141 ccccaaacga aaaacagcaa cgtcagcgcg atataacgga cggttttaac aggtataaat
    16201 gccagtacgc aggcgaagag agtcagaaac gcaagggcta atgtcccagg caattggggc
    16261 aaaatcagca acggaaaaat tccgcaaatt atgcatacgt cgactgtcgt tattttcatg
    16321 cactcctccc tgtttatgga aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt
    16381 tgcgttcccc gaggcacatt cagaagaatt taacgagagg gtgaaaactg ctcaaaactc
    16441 tgcgaggcag cttccagttc ctgattagag aaatgaaaaa agcacccgac aggtgctttt
    16501 ctcgcgttca agtttgagta aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt
    16561 taccaggttt aaagtgagga acgtattttc cttccagttc tactttatcg ccagtcttcg
    16621 gattacgtcc ggtacgtggt gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt
    16681 caatacgctc gccctgcgca agagtcgagg ccatatgctc cagcatctct tttactgcat
    16741 cttcaaccgt cttggcggga atgtgcgatt gctgggtggc aagtctttct atcaattctg
    16801 acttggtcat gattcctccg gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt
    16861 gattaagggc ggccgtagcc gcccttagtg cttgattaca ggacgaaacc tgcaatctgt
    16921 caagtaaact caacaaactt cggaataaaa atcccgaaga gtcagagaat tactcgcctt
    16981 tagctgcttt gaaagcttca gccattgcgt tgttggagaa gtttgcatct tcctgtttgt
    17041 taacagttgc gattgcatct ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga
    17101 ttgcgcggtt tttacgatca acgccggtga atttagcttc aacttcgtcg ccaacgctca
    17161 gaaccaaggt agcgtcttca acgcggtcac gagatgcttc agaagcacgc aggtaacctt
    17221 caacgccgtc agccagttct acggttgcgc ctttagcgtc aactgcagtt actttaccgg
    17281 ttacgatagc gcctttcttg ttcagagcaa cccagttgtt gaacggatct tctgcgagct
    17341 gtttaacgcc cagggagata cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga
    17401 tttcgtcgcc ttttttgtat tcacgaactg cttcttcgcc tgcaacgttc caggagatgt
    17461 cagacaggtg aaccaggccg tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag
    17521 tgatagactt gattttacct tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct
    17581 gccacgggtt agctttgcac tgtttcagac ccagggagat acgacgacgt tcttcgtcga
    17641 tatccagaac cataacttcc actacatcgc caacgttaac aactttggac gggtggatgt
    17701 ttttgttggt ccaatccatt tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt
    17761 caacgaagca gccgtagtca gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg
    17821 gataacgttt agcgatagct acccacggat cttcgcccag ctgtttcagg cccagggata
    17881 cacgggtacg ttcgcggtcg aacttcagca ctttaacagt gatttcgtcg cccacgttga
    17941 cgatttcgct cggatgctta acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt
    18001 caacgccgcc cagatcaacg aatgcaccgt agtcagtgag gttcttaacg atacctttaa
    18061 cttccatgcc ttcctgcagg ttttccagca gctgatcgcg ctctgcgctg ttttcggatt
    18121 cgataacggc acgacgagaa acaacaacgt tgttgcgctt ctgatccagc ttgattactt
    18181 taaattcaag ctctttgcct tccaggtgca gagtgtcacg caccggacga acgtctacca
    18241 gagaacctgg caggaacgca cgaataccgt tcagctcaac agtgaagccg cccttaactt
    18301 tgccgttgat aacaccggta acagtttcag catcttcgta agctttttcc agcgtgatcc
    18361 aggcttcgtg acgtttagct ttctcacggg acagcagagt ttcaccgaag ccgtcttcta
    18421 ctgcgtccag agcaacgtca acttcgtcac ctacctggat ttccagctcg ccctgggcgt
    18481 ttttgaactg ctcagccggg atggcggact cagatttcag accagcgtca accagtacta
    18541 cgtctttgtc gatagcaaca acaacgccac gaacgataga acccgggcgg gtttcgattt
    18601 cttttaagga ctcttcaaag agttgagcaa aagattcagt catgtttaat cttcaggttt
    18661 atatttaacg tccacctggc tccatgccgg atggggttgt ttaacatacc cgctgtcatt
    18721 ccattgcaac gggggtactg caaattcggt cgcttatgcg agagccaatt tctggcgcgc
    18781 gtattgtagc gctttttcaa tcacttgctc aatgcttaag gtggtggaat ccaacactaa
    18841 agcatcggat gccggaacca gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc
    18901 tttgatctcg gccaaaaggc gctcaaagtt aacactaaag cccttctcct gcaactgtag
    18961 catgcggcga tgcgcacgtt cttccgagga ggcgtcaagg aaaattttca ctggcgcatc
    19021 agggaatacc accgttccca tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa
    19081 cgcgcgttgg cgacgcaata atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc
    19141 tgcattcgcc acttcctgag tacgaatttc gccgctgaca tcttcccctt cgaggatcac
    19201 ttccagattg ccattggttg acacaaaacg tacatccaga tgggatgcca gcggtaccag
    19261 cgcatcttct gacgcaacat caacatggtg atgtaatgcc gccaacgcca gtacgcgata
    19321 aattgcaccc gaatccagca gatgccattg caatgcttcc gccatagcct tacacaaggt
    19381 gcctttccct gcaccgcttg ggccatcaat ggtaataacc ggggcaattg ccgtcatctt
    19441 tatctcctta aaacaggcgt accgttaaca taaccgcgcg cattatacgc gccgacgcca
    19501 caaatcgtta acgttgtgtg caaaaaccag ttttgtctgt attcagatgg ggatttattg
    19561 cgcaattata gcgggctggc agaagaccag cccgaaagat gacaatgatt tacttaccag
    19621 aggcgatacg atcacggatg tgttgcgcac gctctgttga tggtgggtga gagtcaaaca
    19681 tggactgggt gcgaccgcca tccagactag ccagtttttc aaagctgcca accagcccct
    19741 gggtgctgat gccacgtttc ttcaacagat caaaggagaa atcatctgct tcggactctt
    19801 tatcacggga gtatttagca ttaatagcgc cttctgcgat atcacccaat tgtgagcggg
    19861 aaagctggga agccacaccg ctggtagctg aaatggcatc gcgtgcggca acgatcgcat
    19921 aagaagcttt catttcagcc agcgagtgac ccaacgcgac gtggcccagt tcatggccca
    19981 gaacaccttc aatttcgttg tcattcatca tgtccatcag gccactgtag acacggacac
    20041 agccgttcgc catcgcccat gcgttgacgt cgctggtcat ataaacctta tagttgaccg
    20101 gcgtgccatt aatattgtta cccaatgctt tggcgatttt tgccagacgt ttaccgtact
    20161 tgctggattt acttgcgact tgattgccgc tgtccatttc ggcacagccc tgattggcaa
    20221 tcgcttttgc atcggcatcg cttaatgttg ctgctttata ggcatttaaa ccggagctga
    20281 tagccatatt ggtatcaata ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg
    20341 tcgcaatcgc cagcagtaat ttagtattct tcatgtgaat catccgttga ttaatcatat
    20401 ataaatatta tgtatcaaca acttacattg acaagcgacg ctaccattaa tcaaaatgac
    20461 tcacaaggtc cgaaaaaaaa cgccgacaat g
//
LOCUS       DAJVQG010000070        20389 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJVQG010000070 DAJVQG010000000
VERSION     DAJVQG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.070"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(132..1781)
                     /gene="actP"
                     /locus_tag="OQM83_002256"
     CDS             complement(132..1781)
                     /gene="actP"
                     /locus_tag="OQM83_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCS1520515.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1778..2091)
                     /locus_tag="OQM83_002257"
                     /pseudo
     CDS             complement(1778..2091)
                     /locus_tag="OQM83_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            complement(2291..4249)
                     /gene="acs"
                     /locus_tag="OQM83_002258"
     CDS             complement(2291..4249)
                     /gene="acs"
                     /locus_tag="OQM83_002258"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCS1520516.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4643..6079
                     /gene="nrfA"
                     /locus_tag="OQM83_002259"
     CDS             4643..6079
                     /gene="nrfA"
                     /locus_tag="OQM83_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCS1520517.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6124..6690
                     /gene="nrfB"
                     /locus_tag="OQM83_002260"
     CDS             6124..6690
                     /gene="nrfB"
                     /locus_tag="OQM83_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCS1520518.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6687..7358
                     /gene="nrfC"
                     /locus_tag="OQM83_002261"
     CDS             6687..7358
                     /gene="nrfC"
                     /locus_tag="OQM83_002261"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCS1520519.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7355..8311
                     /gene="nrfD"
                     /locus_tag="OQM83_002262"
     CDS             7355..8311
                     /gene="nrfD"
                     /locus_tag="OQM83_002262"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCS1520520.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8331..10049
                     /locus_tag="OQM83_002263"
     CDS             8331..10049
                     /locus_tag="OQM83_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCS1520521.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSHSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10042..10425
                     /gene="nrfF"
                     /locus_tag="OQM83_002264"
     CDS             10042..10425
                     /gene="nrfF"
                     /locus_tag="OQM83_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCS1520522.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10422..11018
                     /gene="nrfG"
                     /locus_tag="OQM83_002265"
     CDS             10422..11018
                     /gene="nrfG"
                     /locus_tag="OQM83_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCS1520523.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11360..12673
                     /gene="gltP"
                     /locus_tag="OQM83_002266"
     CDS             11360..12673
                     /gene="gltP"
                     /locus_tag="OQM83_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCS1520524.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12752..13441)
                     /locus_tag="OQM83_002267"
     CDS             complement(12752..13441)
                     /locus_tag="OQM83_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS1520525.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13535..15682)
                     /gene="fdhF"
                     /locus_tag="OQM83_002268"
     CDS             complement(13535..15682)
                     /gene="fdhF"
                     /locus_tag="OQM83_002268"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15263..15265),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCS1520526.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVTERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15880..17346)
                     /gene="mdtP"
                     /locus_tag="OQM83_002269"
     CDS             complement(15880..17346)
                     /gene="mdtP"
                     /locus_tag="OQM83_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCS1520527.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17343..19382)
                     /gene="mdtO"
                     /locus_tag="OQM83_002270"
     CDS             complement(17343..19382)
                     /gene="mdtO"
                     /locus_tag="OQM83_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCS1520528.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEER
                     ALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATAL
                     SRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            complement(19382..>20389)
                     /gene="mdtN"
                     /locus_tag="OQM83_002271"
     CDS             complement(19382..>20389)
                     /gene="mdtN"
                     /locus_tag="OQM83_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCS1520529.1"
                     /translation="PRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYASADTIDVV
                     PEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQIMLTQRSVD
                     AQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTAQRAAEADL
                     NAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSV
                     GQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSI
                     GYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGASAVANLEP
                     Q"
BASE COUNT         4555 a         5431 c         5597 g         4806 t
ORIGIN      
        1 ttatcatttt ctacgactgt caaaaatcgc tcatttttta atgagtttat ttgtttaata
       61 ttttgggaaa aggtgatgca tgctggagag gaagagtatt ccccggtcag gcgaccgggg
      121 aaggggagag attaatgtgc gcggccttgc tcaacgccaa agccggtctg ggagcggata
      181 aactgcgcgc ggaacagttc acgctcgcgc gccccttccg ctgagttatc ggttgccgag
      241 aagaaccaga tgccgaggaa tgccacggtg atagagaaca gcgccgggta ttcatacggg
      301 aagatggctt tttcgtgacc aaggatctgt acccaaatcg tcgggccgag gatcatcagt
      361 actactgcgg taatcagccc cagccagcca cccatcatcg cgccacgcgt ggtcagtttc
      421 gaccagtaca tagaaagcag aatgatcggg aagttacagc tcgccgcgat ggcaaacgcc
      481 agccccacca taaaggcgat gttctgattc tcaaacagca cgccgaggat aatcgcaatc
      541 acgccgagga tcagtacggt gatttttgat acccgcagct cttcacgttc ggtcgcgcct
      601 tttttgaaga cgttagcgta caagtcatgc gaaaccgcgg atgcgcccgc cagcgtcaga
      661 cccgcaacca ccgcgaggat agtggcgaaa gcaaccgctg aaataaaacc gaggaacagg
      721 ttgccgccca ctgcattcgc caggtgaacg gccgccatgt tgttaccacc aatcagatgg
      781 cccgccgcgt ctttatattc cggattcgca ccaaccagca tgatcgcgcc gaagccgata
      841 ataaaggtca gaatatagaa gtagcccata aacccggtgg cgtagaacac gctcttacgt
      901 gcttcgcggg catcgctgac tgtaaagaag cgcatcagaa tgtgcggcaa gcccgccgta
      961 ccaaacatca gtcccagacc cagagagagt gcggagatcg gatctttcac cagcccgccc
     1021 ggcttcatga tgtcgacacc tttcgggtgt accgccatcg cttcactgaa cagattgttg
     1081 aagctaaagc cgacgtgttt catcaccata aaggccataa agctggcacc gaacagcaac
     1141 agcacggctt tgataatctg tacccaagtg gtcgccagca tgccgccgaa caggacgtac
     1201 atcatcatca gcacgccgac cagcaccacc gcaatgtgat agttaaggcc aaacagcagc
     1261 tcgatcagtt tacctgcgcc caccatctgg gcgataaggt aaagcgccac caccaccaga
     1321 gaaccacagg ccgaaagaat acgaatcggc ccttgtttca gacggtaaga ggccacatcg
     1381 gcaaaggtgt agcgccccag gttacgcaga cgttcggcaa tcaggaacaa aatgatcggc
     1441 cagcccacca ggaagcccag cgagtaaatt aagccgtcat agccggaggt aaacaccagc
     1501 gcggagatcc ccaagaatga ggcggcggac atatagtccc cggcaatcgc cagcccgttc
     1561 tggaagccag tgatattgcc gcctgcggtg tagtagtcgc tacgagaacg tacgagtttt
     1621 gatgcccagt aggtaatgcc gagcgtaaac acgacgaaaa tcaggaacat aataatcgcc
     1681 tgccagttcg ttggctggcg ctctacggcc ccgctaatag catccgcggc gttagctgcg
     1741 aaagggagtg tggcggcaag cgccgtcaga actcttttca tgatgcttgt acctcatgca
     1801 ggacttcatt attaagacgg tcgaattcgc cgttcgcccg ccagatgtag ataccggtga
     1861 gaacaaagga gatcacaatc actccaacac caattggaat accgcgtgtg acgctggtgt
     1921 tcggattcag cggcgtgcca gccagccggg cgcaaaggcg atcagtaaaa taaagccgat
     1981 ataaactgcc agcataataa tcgacaggat ggtggcaaac cgttgccgtt tttcgactaa
     2041 ctccctgaaa tgcgcattgt cttctatccg ctgataaata gtgccattca tcacagattc
     2101 tccagaggta atgtagggat tgttttaatt cccgctccct tatgggagaa ggttaacgct
     2161 cgggtaaccc ttgccgaatg taggccggat aaggcgttta cgccgcatcc ggcaatcaat
     2221 gcctgatgcg acgctgtcgc gtcttatcag gcctacaaac cgttaccgac tcgcatccgg
     2281 caattgtggg ttacgatggc atcgcgatag cctgcttctc ttcaagcagc ttctcgacta
     2341 cgccaggatc ggcaagcgtc gaggtatcgc ccaggttgct ggtatcgccc gccgcaattt
     2401 tgcgcagaat acggcgcata attttgccgg agcgggtttt aggcagggag tcggtccagt
     2461 gcagcacgtc tggcgtcgcc agcgggccaa tctctttacg cacccagttg cggacttctg
     2521 cgtacagttc tggtgacggt tcctccccgt gattaagcgt gacgtaggcg tagatcgcct
     2581 gacctttaat attgtgcgga atacctacta cggcggcttc ggcaatcttc ggatgcgcca
     2641 ccagcgccga ctcaatctct gccgtcccca gacggtgacc ggagacgttc agcacgtcgt
     2701 ccacacgccc ggtgatccag taatagccat cttcatcgcg acgcgcgccg tcgccgctga
     2761 aatacatatt tttgaaggtg gagaagtagg tctgttcaaa acgttcgtga tcgccaaaca
     2821 gcgtacgcgc ctgacccggc caggagtcgg tgatcaccag gctgccttcg gtagcgcctt
     2881 ccagcgggtt accttcgtta tcgaccagcg ccggttgcac gccgaagaac ggacgtgttg
     2941 ccgaaccggc tttcagctcg gtagcgccag gcagcggcgt gatcatgaaa ccgccagttt
     3001 cggtctgcca ccaggtatcg accaccggac atttctcgtt gccgattttt ttccagtacc
     3061 actcccacgc ttccgggtta attggctcgc ccacggaacc gagaatgcgc agcgacgaac
     3121 ggtcggtacc ctcgatcgct ttatcgcctt ccgccatcag cgcgcggatc gccgtgggtg
     3181 cggtatagag aatattgacc tgatgcttgt ccaccacctg cgccatacgg gcaggcgtcg
     3241 gccagttggg tacgccttca aacatcagcg tggtcgcacc gcaggccagc gggccgtaca
     3301 gcaagtaact gtgtccggtc acccagccca catcggcggt gcaccagtag atatcacccg
     3361 gatgataatc aaagacatat ttaaaggtca gcgccgcgta caccagataa ccgccggtag
     3421 tatgcagcac accttttggc ttaccggtag aaccggaggt gtagagaata aacagcggat
     3481 cttcggcgtt catcttttcc gcctggtgct gatcgctcgc ttgctcaacc aggtcgtgcc
     3541 accacaggtc gcgcccttcc tgccagtcaa ttttcccgcc agtacgcttc agtaccacca
     3601 catgctctac gctggtgacg ttcgggtttt tcagcgcgtc atcaacgttt ttcttcagcg
     3661 gaatactgcg cccggcacgc acaccttcgt cggaagtgat caccagtcgt gagttggaat
     3721 caataatgcg cccggcaacg gcttccggcg agaagccgcc gaaaatcacc gaatgcaccg
     3781 cgccaatgcg ggcgcaggcc agcatcgcaa ccgcggcttc cggcaccatc ggcatataaa
     3841 tcgccaccac atcacctttt ttaatgccca gctcgagcag ggtattggcg aagcggcaga
     3901 cgtcgcggtg cagctcttta tagctgatat gtttgctctg gctggcgtcg tcgccttccc
     3961 agatgatggc ggtacgatcg ccgttttctt gcagatggcg gtcaaggcag tttgccgcca
     4021 gattcagcgt gccgtcctcg taccatttaa tggacacatt accgggggca aaggaggtgt
     4081 ttttcacctt ctggtaaggt ttgatccagt caagaatttt tccctgttcg ccccagaagg
     4141 tatcaggtac gttaatagat tgttgataca tcgcctcgta ctgctgaggg tttatcaggc
     4201 aacggtctgc gatgttggca ggaatggtgt gtttgtgaat ttggctcatg cttttgttct
     4261 ccttgtaggg tgttaataat atgtggcata agcgttaaat gtaggggtat tggcagtttt
     4321 gtttagtatt tgggcgacag atcacgcaaa agtagaattg tgcaaataaa cggcagggta
     4381 atttttgaag gtcagcaaca aaagttgatt aattctttga ggaacatgca gttatgcatt
     4441 gctgttgaaa aagaggaaga tactaactaa ctctaaagtg gtattttaca tgcacttaca
     4501 attgattaaa gacaacattc acagtgtggt tatttgttac acataggggc gagcaatgtc
     4561 atgacagtgt aggtgcggtt actgtcgtga aaaacaataa aaaccgccat tgtaacaatg
     4621 gcgcaattcg gatgaagccc ctatgacaag gataaaaata aacgcacgcc gtatcttcag
     4681 cttattgatt ccttttttct ttttcacttc tgttcacgct gaacaaacgg cagctcccgc
     4741 aaaacctgta actgtggaag cgaagaatga aacctttgcc ccgcagcatc ccgatcaata
     4801 tctctcctgg aaagccacct cggaacagtc agagcgtgtt gacgccctgg cggaagatcc
     4861 acggctggtg atcctgtggg cggggtatcc cttctcgcgc gattacaaca agccgcgtgg
     4921 acatgccttt gctgtgaccg atgtgcgtga aaccctgcgt accggtgcgc cgaaaaacgc
     4981 tgaagatggt ccgctaccga tggcgtgctg gagttgtaaa agcccggatg tggcgcgtct
     5041 gatccagaaa gacggcgaag atggctactt ccacggtaag tgggcgcgcg gcggcccgga
     5101 aatcgtcaac aacttaggtt gtgccgactg ccataacacc gcctcaccag agttcgccaa
     5161 aggcaaaccg gagttaaccc tttcccgtcc gtatgcggct cgcgcgatgg aagccattgg
     5221 taaacctttt gagaaagccg gacgtttcga ccagcaatcg atggtttgcg gtcagtgcca
     5281 tgtggagtat tacttcgacg gcaaaaacaa agcggttaaa ttcccgtggg atgacggcat
     5341 gaaagtcgaa aatatggagc agtattacga caaaattgcc ttctctgact ggactaactc
     5401 cctgtcgaaa acgccaatgc tgaaagcgca gcacccggaa tatgaaacct ggacagcggg
     5461 cattcacggt aaaaacaacg tgacctgtat cgactgccat atgccaaaag tgcagaacgc
     5521 cgaaggcaaa ctctataccg accataaaat tggtaatccg tttgataact tcgcccagac
     5581 ttgtgcgaac tgccataccc aggacaaagc tgccttgcaa aaagtggtcg cggaacgtaa
     5641 gcagtcgatt aacgacctga aaatcaaggt tgaagatcaa ctggttcacg ctcacttcga
     5701 agcgaaagca gcgctggatg caggcgcgac ggaagctgaa atgaagccaa ttcaggacga
     5761 tatccgtcat gcccagtggc gctgggatct ggcgatcgct tcccacggca ttcatatgca
     5821 cgcaccggaa gaaggtttac ggatgctcgg tacggcgatg gataaagcgg cggatgcacg
     5881 caccaaactg gcgcgcctgc tggcgaccaa aggcatcacc catgaaatcc agatcccgga
     5941 tatctcaacc aaagagaaag cccagcaggc cattggcctg aacatggaac aaatcaaggc
     6001 cgagaagcag gacttcatca aaacggtgat cccgcagtgg gaagaacagg cacgtaaaaa
     6061 cggtctgtta agccaataac cccgttccgc ctcgcaaggg gcggaaaaca caatggagtg
     6121 aatatgagcg tattacgttc gttattaact gccggggtgc tggcgtcggg cctgttgtgg
     6181 agcctgaacg gaattaccgc tacccctgcg gcgcaggcat ctgatgatcg ctacgaagtt
     6241 acccagcagc gtaacccgga tgccgcctgt ctggactgtc ataaaccaga taccgaaggt
     6301 atgcatggca aacatgcttc cgttatcaac ccgaataaca aactgccggt cacctgcacc
     6361 aactgtcacg gccagccatc accgcaacac cgcgaagggg tgaaagatgt gatgcgcttt
     6421 aacgagccga tgtacaaggt tggagagcag aacagcgtct gtatgtcctg tcatctgccg
     6481 gaacagttgc aaaaagcgtt ctggccgcac gatgtccacg taaccaaagt ggcgtgcgcc
     6541 agctgccatt ccctgcatcc acagcaagat acgatgcaga cgttaagtga caaaggacgg
     6601 attaagattt gcgtcgattg ccacagcgat cagcgcacca atccgaactt taacccggcg
     6661 tctgttccgt tgcttaagga gcagccatga cctggtctcg tcgccagttt ctcaccggcg
     6721 tcggcgtgct ggcagccgtc agcggcaccg cagggcgcgt ggtggcgaaa acgttgaata
     6781 tcaatggggt gcgttacggc atggtgcatg acgagtcgtt atgcatcggc tgtaccgcct
     6841 gtatggatgc ttgtcgggaa gtgaacaagg tgccggaagg cgtctcgcgc ctgacgatta
     6901 ttcgtagcga gccgcagggc gaatttcctg atgtgaaata tcgcttcttc cgtaagtctt
     6961 gccagcactg cgatcatgcg ccgtgcgttg acgtctgccc gaccggtgcg tcttttcgtg
     7021 atgccgctag cgggattgtc gatgttaatc ctgatctctg cgtcggttgt cagtactgca
     7081 tcgccgcctg tccgtaccgc gtgcgcttta tccatccggt cacgaagacg gcggacaaat
     7141 gcgatttctg ccgtaaaacc aatttgcagg ccggtaagct gcccgcgtgt gttgaggctt
     7201 gcccgacgaa ggcgctgacg tttggcaatc tggacgatcc taacagtgag atttcgcaac
     7261 tgctgcgcca gaagcctact taccgctaca agctggcgct gggaaccaaa ccgaagctgt
     7321 accgcgtacc gtttaaatac ggggaggtga gccaatgacg cagacttccg catttcattt
     7381 tgaatcgctg gtgtgggact ggccgattgc catctacctg tttttgattg gtatttctgc
     7441 cggtctggtg acgctggccg tgctgttacg tcgcttctac ccgcaggcgg gcggtgcaga
     7501 cagtacgttg ctgcgcacca cgctgattgt cgggccgggc gcggtgatcc tcggtctgtt
     7561 gatcctcgtc ttccacctga caagaccgtg gaccttctgg aagctgatgt tccactacag
     7621 ttttacctcg gtgatgtcga tgggggtgat gctgtttcag ctctacatgg tggtgctggt
     7681 gctgtggctg gcgaaaatct ttgaacatga tttgcttgcc ctgcaacaac gctggttgcc
     7741 gaagctgggg atcgtgcaaa aggttctgag cctgctgacg cccgttcatc gcggactgga
     7801 aacgctgatg ctggtgttgg cggtgctgct gggggcttat accggctttc tgctgtcggc
     7861 gctgaaatcg tatccgttcc tcaataaccc gatcctgccg gtgctgttcc tcttctccgg
     7921 catctcgtcc ggtgcggcgg tggcgctgat cgccatggcg atacgccaac gcagtaaccc
     7981 gcattccacg gaagcgcact ttgttcaccg gatggaaatc cccgtggtat ggggtgaaat
     8041 cttcctgctg gtggcgtttt ttgtcggtct ggcgctgggc gatgacggta aagttcgtgc
     8101 gctggtggcg gcattaggtg gcggtttctg gacgtggtgg ttctggcttg gtgtcgccgg
     8161 gctggggctg attgtgccaa tgttgctcaa accgtgggtc aatcgcagtt ccggcattcc
     8221 tgccgtgctg gcggcgtgtg gggccagtct ggtcggcgtg ttgatgctgc gctttttcat
     8281 tctctacgcc ggacagttga cggtggcgta agccagaaaa gaggtggttt ctggacgtat
     8341 tccttcctga agtcggtttt ctggcgttgt tgttaagtct cggggtcaac gtgttgaccc
     8401 cgttgacggc ctttgcggga gtgcggttgc gctggcctgc catgatgcga ctcacttgca
     8461 tcggcattct ggcgcagttc gcgctcctgc tgctcgcctt tggcgtactg acgtattgtt
     8521 ttctcatcag cgatttctcg gtcatttatg tcgcgcaaca tagccatagc ctgctgtcgt
     8581 gggaattcaa gctggcagcg gtgtggggcg gccatgaagg ttcgctgctg ctttgggtgc
     8641 tgctgctttc cgcctggagc gcgctgtttg cctggcatta tcggcagcaa accgatccgc
     8701 tatttccgct gacgttagcc gttttatctc tcatgctcgc cgcactgcta ctgtttgtgg
     8761 tgctgtggtc cgatcccttc gtgcggatat ttccaccagc aatcgaaggc cgcgatctca
     8821 atccgatgct gcaacatccc ggtcttatct ttcatccacc gctgctttac cttggctatg
     8881 gcggtttgat ggtagcggcg agcgtggcgc tggcgagtct actgcgcggc gagtttgatg
     8941 cggccagcgc ccgaatttgc tggcgctggg cgttacctgg ctggagtgca ttaacggcgg
     9001 ggatcatcct cggttcctgg tgggcctact gcgaactcgg ctggggcggc tggtggttct
     9061 gggatccggt ggaaaatgcc tctttattac cctggctttc tgccactgcg ctgctgcaca
     9121 gtttatccct gacacgccag cgggggattt tttgccactg gtcgctgttg ctggcgatag
     9181 ttactctgat gctgtcgctg ctgggcacct taattgtccg ttctggcatt ctggtttcgg
     9241 ttcatgcgtt cgcactggat aacgtccgcg ccgtgccgtt gttcagcctg tttgcactga
     9301 ttagccttgc gtctttggct ctgtatggct ggcgagcgcg ggacggtggc ccggcggtgc
     9361 gtttttcggg gttatcgcgg gaaatgttaa tcctcgctac gctgttgctg ttttgcgcag
     9421 tgctactgat cgtgctggtg ggaacgcttt atccgatgat ttacggtctg ctgggctggg
     9481 gacgcctctc cgttggcgcg ccgtatttta accgcgcgac gttaccgttt ggtctgttga
     9541 tgctggtggt gattgtgctg gcgacgtttg tctctggcaa acgcgcgcag cttccggcgc
     9601 tggtcgctca tgcgggcgtg ctgttatttg ccgcagggat cgtggtttcc agcgtcagcc
     9661 gtcaggagat cagcctgaat ttacagccgg gtcagcaggt gacgctggca ggatacacct
     9721 tccgttttga gcgcctcgat ctgcaagcca aaggcaatta caccagcgaa aaagcgatag
     9781 tggcgctgtt tgaccatcag caacgcattg gtgaattaac gccggagcgg cgtttttatg
     9841 aagcacgccg tcagcaaatg atggaaccgt caattcgctg gaacggcatc catgactggt
     9901 atgcggtcat gggtgaaaaa accggagcgg atcgttacgc ttttcgtttg tatgtacaaa
     9961 gcggtgtgcg ctggatctgg ggtggaggat tgttgatgat tgcgggcgca ttgttaagcg
    10021 gatggcgggg gaagaagcgc gatgaataaa gggcttctca cgctactgct gttatttacc
    10081 tgttttgcgc gcgctcaggt cgtagacacc tggcaattcg ccaatccgca acaacagcaa
    10141 caggcgttaa atattgccag ccagttacgt tgtccgcagt gccagaatca aaacttactc
    10201 gaatccaatg cgccggtggc ggtcagtatg cgccatcagg tttatagcat ggtgtcggag
    10261 gggaagaacg aagtcgaaat cattggctgg atgaccgaac gctacggaga ttttgttcgc
    10321 tataacccac cgttaacggg tcagacgctg gtgttgtggg cgctgccagt ggtgttgtta
    10381 ttgctgatgg cactgatcct ctggcgagtg agggctaagc gatgaaacag cctcaaatac
    10441 cggtgaaaat gctgacaaca ctcacgatat tgatggtatt cctctgtgtc ggcagttatc
    10501 tgttaagccc aaaatggcag gcggtacgtg cggagtatca gcgtcagcgc gatccgctac
    10561 atcagtttgc cagccagcaa accccggaag cgcagcttca ggcattgcag gataaaatcc
    10621 gcgctaatcc acaaaacagc gaacaatggg cgttactggg cgagtactat ctgtggcaaa
    10681 acgattacag caattcgctg ctggcgtatc gtcaggcgtt gcaactacgt ggtgagaacg
    10741 ctgaactgta tgcggcgctg gcgacggtgc tttattacca ggccagccag catatgaccg
    10801 cccagacttg cgcaatgatc gacaaagccc tcgcgctgga ctctaacgaa atcaccgctc
    10861 tgatgctgct ggcttccgat gcgtttatgc aggcgaatta cgcgcaagcc atcgaactat
    10921 ggcaaaaagt gatggatctc aactcacccc ggatcaaccg gacacagctg gtcgagtcga
    10981 ttaatatggc aaaactgttg cagcggagat cggattaatc atctggtgat gaagattatg
    11041 tcattgagct gcataaaaaa taatcgaatg aacatatgcc aaaaataatc actaatcagt
    11101 attattgcag attaaacaaa taaaaatctt tccataacaa atggttattc attaatcctg
    11161 ccataaactc gcgttattat gcattaatgc agcgaaaagc tctgttgtta aagggttgcg
    11221 caacataccg cgcaaatgat actgatcata agcgttaaaa aaatctacaa accaacgcaa
    11281 cacaattcat gccctggcag tatgtcacgt tctcgcgttt ctgaacgggg aacggcgctc
    11341 cattgaggaa gttattctga tgaaaaatat aaaattcagc ctggcctggc agattctgtt
    11401 tgctatggtg ctgggcattc tcctgggaag ctacctgcac taccatagcg acagccgcga
    11461 ctggctggtc gtcaatttgc tctctccggc gggtgatatc ttcatccatc tgatcaaaat
    11521 gattgttgtg ccgattgtga tctccacgct ggtggtgggt atcgcgggtg ttggtgatgc
    11581 caaacagctc gggcgtattg gcgcgaaaac cattatttac ttcgaggtga tcaccaccgt
    11641 cgccatcatt ttggggatca ctctggcgaa cgtcttccag cccggtgccg gggtggatat
    11701 gtcgcagctg gcgaccgtcg atatctcgaa atatcagagc actacggaag cggtacaaag
    11761 cagttcccac ggcattatgg gcacgatttt gtcgctggtg ccgacgaaca ttgtggcgtc
    11821 gatggcgaaa ggcgaaatgc tgccgatcat ctttttctcg gtgctgtttg gtctggggct
    11881 ttcttccctg cccgcgacgc atcgtgaacc gctggtgacc gtgttccgct ccatctctga
    11941 aaccatgttt aaagtgactc acatggtgat gcgttatgca ccggtgggtg tgtttgcgct
    12001 gattgcggtg acggtggcta actttggttt ctcttctctg tggccactgg cgaaactggt
    12061 gctgctggtg catttcgcca ttctgttctt cgcgctggta gtgctgggaa ttgtggcgcg
    12121 cctgtgcggg ttaagcgtct ggatcctgat tcgtattctg aaagatgagc tgattctggc
    12181 gtactccact gccagctctg aaagcgtact gccgcgaatt attgagaaga tggaagccta
    12241 cggagcaccg gcgtcgatca ccagcttcgt ggtgccgacc ggttactctt ttaaccttga
    12301 tggttcgacg ctgtatcaaa gtattgccgc tattttcatc gctcagctgt atggcattga
    12361 cctgtccatc tggcaggaaa tcattctggt gctgacgctg atggtgacct cgaaagggat
    12421 tgctggcgtg ccgggcgtgt cgtttgtggt gctgctggca acgctgggta gcgtaggtat
    12481 tccgctggaa ggtctggcgt ttattgctgg tgttgaccgt atcctcgaca tggcgcgtac
    12541 tgcgctgaac gtggtgggta atgcgctggc ggtgctggtg attgccaagt gggaacacaa
    12601 atttgaccgc aaaaaggcgc tggcttatga gcgtgaagtg ctgggcaaat ttgataaaac
    12661 tgcggatcaa taattgaaga ttgccgggga tatccacccg gcaatgtgtg aatgcctgat
    12721 gcgacgcttg ccgcgtctta tcaggcctac cttaaccgtt ggttaatttt tcaaactctt
    12781 cacacccggt atcaaaccct tccatacagc tcaggttcaa ccagtgcagc gccttttgct
    12841 tgttcttctc gataaaacct tcttcaccgt ttaagaacat cattcccgcc cagtactcgg
    12901 aataaccggt tcgggaaatt gcagagctgc gtttgaaata ccaggttgcc ttgtcatcgt
    12961 cggccttaat gcccacgccg ttggcgtaaa tcaaaccaag cagcatttgg gcatcgacgg
    13021 cagagtcgtt ctccagatct tccgaggcgt tttctaacag cgaaatagcc tttggataat
    13081 ccggtttacc cgcttgagta tttaccagaa tatgcgccag cgttacttca ccttctttac
    13141 tgcccgcttg cgccgctttc tctgcaagaa cttttgcttg tggatagtcc agactgaccg
    13201 gattggtgat tttgatctgc gccagcagcg cgcaggcatc agcatcaccg ctgtcggcgg
    13261 ctttctgtgc ccaatattcg gctttgctca aatcgccgga gctaaaccag ctgtcggcaa
    13321 gaaaatattg tgcgcgtcgg tccccggcct ctgctgcctt taaatactgg ctgccaggct
    13381 cggagtcgtt ggcgtgggca aagaatgtca aaaacaacat caatgcgata atttttttca
    13441 ttttgataat acgattgggt agggagtaac cagtataaag agttcgaatg gataaaaaaa
    13501 cagcctccga aaggaggctg tagaaaggac tgtattacgc cagtgccgct tcgcgcaggc
    13561 gagttttcaa cttgttgtac tcgtcaatca cgtactgctc ggcggcgcgc tgatcggcga
    13621 tcggctcgac gcgcacggcg cagtatttgt attccggcgt tttcgtaatc gggcttaagt
    13681 tttcggtaac cagctcgtta caggcaccaa tccaccactg gtaggtcatg taaatcgccc
    13741 ctttgttcgg acgatcgctg acctgcgcac gggtgataat tttgccttta cgcgagtgca
    13801 cccaaaccaa tgcctcatct tcaataccca gacgtttggc gtcttcggta ttgatttgtg
    13861 cgtagccagg ttcatcagcc agcgccgcca gtgccgcaca gttaccggtc atcgaacggc
    13921 aagagtagtg accaacttca cgcaccgttg acagtaccat cgggtactcg tcggtgagtt
    13981 tgtcgattgg cgctacccag tcgcaggtga agaactgcgc cagaccgttc ggggtatcaa
    14041 acttctcttt aaacagataa gaagtcccct gatcggcatc tgaagtatcg cggcaaggcc
    14101 actgaatgaa gcccagttcg cccattttct cgtaagtcgc accgtagaaa tccgggcaca
    14161 gatgacgcaa ctcatcccag atctcctggg tgttgttgta gtgcatcgga taacccatac
    14221 gggtggcgat ttcactgatg atttgccagt ccgttttcag atcccatttc ggttcaactg
    14281 ctttgaagaa acgctggaag ccacggtcag ccgcagtaaa cacgccttca tgctcgcccc
    14341 acgacgttga cggcaaaatg acatccgccg ccgacgcggt tttggtcata aagatgtcct
    14401 gaacgataac cagttccaga tcttcaaagg ctttacgtac tgccgacagc tccgcgtcag
    14461 tttgcagcgg atcttcgccc ataatgtacg cggcacgcac ttcgccatgc gctgcgcggt
    14521 gcggcagctc gctgatgcga taaccggtat gagcaggcag gctttccacg ccccaggctt
    14581 tggcgaattt ctcgcggtta gccggatctt tcacgtactg gtatcccgga tacgtatccg
    14641 gcagcgcgcc catatcgcag gcaccctgca cgttgttctg accacgaacc gggtttacac
    14701 ccgcatgcgg cttaccgagg ttaccagtca gcatcgcgag gctggtcaga gaacgcacgg
    14761 tttccacgcc ctggtagaac tgggttacac ccatgcccca caggatggcg gcgcttttcg
    14821 cctgggcata catccgtgcc gcctgacgaa tctcactggc gctgacgccg gtgatatctt
    14881 caaccgactc cggcgtgtag ccttcaacga ttttacgata ctcttcaaag ccttctgtac
    14941 gggaagcgac gaacgctttg tcgtacagat tttcttcaat aatgacatgg cccatcgcat
    15001 tcaacagcgc gatgttcgag ccgtttttca gtgcaatgtg catgtcagca atgcgcgcgg
    15061 tttcaatttt gcgcggatcg cagacgataa ttttcgcccc gttacgttta gcgttaatta
    15121 cgtgattcgc cacgattggg tgggaatcag ccgggttgta cccgaaaacg aacactaaat
    15181 cggtattatc aatttcgtta atagcattgc tcattgcgcc attaccgacc gattggtgca
    15241 gacctgcaac cgatgggccg tgtcagacac gagcgcagca gtcaacgtta ttggtaccaa
    15301 taacggcgcg cgcaaatttt tgcattacat agttggtttc gttacccgta ccacgcgagg
    15361 agccggtcgt ctggatggca tccggaccgt acttctcttt gatggcgctc aggcgctcgg
    15421 taacgtaatt cagtgcctca tcccaggaaa caggttcgag tttgccgcca cgctggcgac
    15481 ggatcatggg ggttttcagg cgcggggtca ggatctgggt atcgttaatg aaatcccagc
    15541 cataataacc cttcagacac agggtaccct ggttggtttt cccctgcgct gcctccgccc
    15601 ggacgatttt gccgttatcg acgaccaggt tgattttgca acctgatgcg caataggggc
    15661 aaaccgtgac gacttttttc atcggtctcg ctccagttaa tcaaatcacg catacgcgct
    15721 ctcgactaca gtatgcatct tttatgccac attttatttg gggtcattcc ctgatattac
    15781 gggcactatt tattcaaaac tctgacgaaa aacaggctgt cgtcagtttt gacgtgacga
    15841 aacgaaatac cgcgtgacag ccatcacgcg gcagacattt tattttttct cgacgacggg
    15901 agccgcctga tacccgccgc ccagcgattt catcaactga atgctttgga tcacccggcg
    15961 gctgtccagc atcagtaatg acatctcttc ggcaagtact ggcaaccggg cttcagtggc
    16021 ctgtaagcgg ctggttaagc cgcgctgata ggcggcctcg gcagcgcgct gggtgaagcg
    16081 cgttgcttcc acgcgttcag cctgcatttc tcgctcgtcg ttgagcgttt gcagacgcgt
    16141 gccgttgacg gcaacgtcac gcaccgcgtt cagtactgac tggttgtaac gttcaatcat
    16201 catgttgctg gcggcgcgcg tgccttcgag attggcattc aaccgtccac cgtcaaacag
    16261 cggcaatttc agacccggga tgaagttgaa ctggcgactg gtttttttga ataaggtatc
    16321 caggtggatg gcatcaaggc cgaaaaacgc tttgatatca aagctcgggt agaacagcgc
    16381 ccgcgcggaa tccacctgat ctaatgacgc ctgaacatac cagcgcatgg cttgcagatc
    16441 cgggcgtctg gcgagcaact cataagagag tgtcgccgga atgccggtct ggactcgtgg
    16501 taatgccacc ggtttgatct ccggcatatc gctggccccc gcgccaatca atgcacgcag
    16561 agattctcgc gtttcggtga tttgcccttt gacggcagca atttgtttat cgaccgccag
    16621 aatctgtgcc cgcgcgccgt ggaaaggcac ttgtgcttcc agaccgtgcg ccactttact
    16681 ctggtgcgct ttcaccgcgt aatcaatcac atcgcgagtt tgttctaaca gatcgagcat
    16741 ctgatagctg gcctgcatac tgtaataaag ctgcgctacg cccgtggtca gcgatagctc
    16801 tactgctgcg gtttctgcca gcgcggcatt atgcgcgcca atggcggcgg caaccgctga
    16861 gcgatgcaca ccccacaaat caagatccag tccggcaaac aaacctactg tggcttccgt
    16921 atagtacggc ccgtccatac ccagtgcggg cgcatccatc gcataagggc ttaaaaagcc
    16981 gttcgccgag acgcgttggc ggttgagcat ccctaacgct gcgacctgta attgtgaacc
    17041 ggcatctaac aaatcggcct gggactgcgc tttttcttcc cgcagtttcg cttcggcgag
    17101 ggtgtgtgaa ccacttagcg tccgttggat cagcgcatcc agctgcgggt cattgagttg
    17161 tttccaccac tgcgcctgcg gccagccgga gctggcaaga tgaatatcat cggccagttt
    17221 gatttgttcc ggtttgagct gttgatgagg cgcagaatcc ttgcgtacca aggcacagcc
    17281 ggaaatcagc gtcgtgctgc cgagaatgct gcacaacagc agacgtgaaa gttgacgatt
    17341 gatcattgcg tggctccttg tgcctgttcc gtggcgggcg ttaatgccgg ggctgtccag
    17401 tctggcaagc gggcgaaaag ctggcagacg tgttgctcct gttttaataa ggtgggcagg
    17461 atggcctgcg aggcgggtgt ctcttcaagc gttatttgag gagaacggct gagtgcggtt
    17521 gccagaccgg cagcgtattt ttccagggcg tcggcaaact gacctgctct gtccggctgt
    17581 agtgcgttat ccagcgcctg cgccgaattc caggtggcat cccaggcgtg aaggatatcg
    17641 cggccctgac gaataaccgt ttgcgaacgt tcaatcagta aggcgcgttc ttcgctgtcc
    17701 agttgacgct ccagcgccac gcgttggcac atctcttcac aggcattaaa cgccgcatgc
    17761 agaccgatac gaatttgcag ataagtgcgc agagcctggc gtgggatccg cattacttta
    17821 cttaacatac ccagcgcgcc agccagtttt tgcggcagcg tgcgcgcttc actttcaggc
    17881 cagacaaagg tgtaaatcac cgcggacacc acggtaccaa tgatgatacc cagggcgcga
    17941 tcgcgaattt ccaccaggtc gtacactggg ccaaaaacgt tttcgagcgt ggcgagcgcg
    18001 aaggtgacca ccatctgtgt gccgatataa gaagagcgtt cagagctggt ggcaatccat
    18061 gcgcccaaca ggaaaatcgg tgccagcaca aacagcaatt cgacaatatt gtccagccag
    18121 ggcatgacca gtagcgtgaa taacagcgcc agaatcgcgc cgcaaaaggc cccgccaaaa
    18181 cgcagcacca tcttctggta cgacgaaccg acatttggat tagagacgat cacgcaggtc
    18241 agcatacagg tgtgaatgcc ttcccagtcc acgccgctgt aaaaggtgta acagatcaaa
    18301 caggcgagca atgtttttac cgcgtagcgt atatagtctg gattggtaaa agcatcggcg
    18361 accattgatg gcggtttggc ggcgggcgtt ggcggcgtat tcgggttcat ctggcccagt
    18421 tgtaacaacg tctggcagat attctccagg ttacattccc gtgccgccac cgcttcactt
    18481 tcactgatcc gccagtcgct ttgccagcac tgaccttcag caacggcatg ctgcagcttg
    18541 ttgatttctg aggctaattt ttgccgaaat tcaataattg cctgagaatc agcaaaagag
    18601 gtgggatcgt agcgattcag cgtcgagtaa atgtaggtta ccgttgccac gcagctttgc
    18661 caccatgcgc tttgagttcg ccagttggca tcgtccgcga gacaaaagac attcagtttt
    18721 tgcagcgcca gcgcctctct ttcaatccgc gtttcgggta gcggtgcgag gctgtctgtc
    18781 aggtgactaa tggcatcatc aagccgatca ttaagcgcct gatgcatttg cgaaatggca
    18841 cgactgggaa accacagcac gccgattaac gtcatcagca aggttggata gaggccaaca
    18901 acgatacacc acagcgttaa gcgcacgacc acttccggat agtcgagcat ggcggggaag
    18961 gtttgcccgt aaatggcgac aatggcgaca gcgaaaaaga ccagtcccag ccgatgggtg
    19021 cgcatcaaaa acatgcggcc catcaggatc ggcccggcga tgatcaatcg gatcaacggt
    19081 tcgccgtatg accatttgta gatcaaaaac aggctgccga tctccagcac cgtggcaacc
    19141 acaaacaaga tcgccacaaa tttggtgtaa aacgcgttcg actgaatacc gtaaaacagc
    19201 actgccagcg ataacgccac aaaagggatc tcaaaagtca tcgagatcag aatcaccagc
    19261 aggcagccta cccagagttg cacgatctgc ggcacgcggc cgggtcgccg ctcgcttaac
    19321 tcttcatgaa agaacgccag cagcctgacc atcggtaatg gcagggagtt gagcgcgctc
    19381 attattgcgg ctcaagatta gcgactgccg aagcgccgat gcggaacatt tccgggtcag
    19441 gtttatcgac catgattttg accggaaaac gctgggcaac gcggacccag ttaatagagc
    19501 gagaaacttt cggcaggccg cccagcacca ggccgccgtc atccggtagc acgccgtagc
    19561 caatcgaatc cactttaccc tcgaaggttt tgccgctgtc actcatcagg cgaatcgttg
    19621 cgggtgtacc tgagcgaata tttttcagat cggtttcgcg gaagttggcg atcacgtacc
    19681 agtgacgagt gtcgattagg gtaaaaatag ggcgcatggc agaagcaaat tgcccgacgg
    19741 aggttttgag ggaaatgacc cggccatcaa acggcgcgcg aacggtcgcc atttccagat
    19801 gcagtttggt cagggcaata tccgcttcga ccgccgcacg ttgcgccacc agcgcatcga
    19861 cgccgctgac ggcgctggcg gctgactgcg cctgtaacaa tacggcatta agatcggctt
    19921 ctgcggcgcg ctgcgcagtt cttgcacggt caacgtcttc cgctgagaca aaaccctctt
    19981 tcagtaatgg ctcggtacgg cgtaatgtat ctgtggcctg tttcgcggcg gcacgggctt
    20041 tttctaccgt ggcattaatc gagtcggcac caaactgttg cgcatcaacg ctacgctggg
    20101 tgagcataat ttgcttatcc agcgccgcga gggaggcttc agctttcgcc agattggctt
    20161 cgtacgggcg cgggtcaatg cggaacagca aatcgccctg tttgactgcc tggttgtcgg
    20221 tgaccgccag ttctacaatg cggccgctga cttccggcac cacatcaatg gtatctgctg
    20281 acgcgtaagc gtcattagtt gatggcgcac tgtctacgcg ccagataacg aaaacaaggg
    20341 caaccagcgc caatgcaacc actaacagag cagggaattt actgcgagg
//
LOCUS       DAJVQG010000071        20231 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJVQG010000071 DAJVQG010000000
VERSION     DAJVQG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.071"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            690..1460
                     /locus_tag="OQM83_002272"
     CDS             690..1460
                     /locus_tag="OQM83_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCS1520530.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1451..1924
                     /locus_tag="OQM83_002273"
     CDS             1451..1924
                     /locus_tag="OQM83_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCS1520531.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            2032..2571
                     /gene="dnaT"
                     /locus_tag="OQM83_002274"
     CDS             2032..2571
                     /gene="dnaT"
                     /locus_tag="OQM83_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCS1520532.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2574..3311
                     /gene="dnaC"
                     /locus_tag="OQM83_002275"
     CDS             2574..3311
                     /gene="dnaC"
                     /locus_tag="OQM83_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCS1520533.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3360..3854
                     /locus_tag="OQM83_002276"
     CDS             3360..3854
                     /locus_tag="OQM83_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCS1520534.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            4108..6399
                     /gene="opgB"
                     /locus_tag="OQM83_002277"
     CDS             4108..6399
                     /gene="opgB"
                     /locus_tag="OQM83_002277"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCS1520535.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6538..7560)
                     /gene="lgoD"
                     /locus_tag="OQM83_002278"
     CDS             complement(6538..7560)
                     /gene="lgoD"
                     /locus_tag="OQM83_002278"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCS1520536.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7699..8613
                     /locus_tag="OQM83_002279"
     CDS             7699..8613
                     /locus_tag="OQM83_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1520537.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8828..10179
                     /locus_tag="OQM83_002280"
                     /pseudo
     CDS             8828..10179
                     /locus_tag="OQM83_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10228..11883)
                     /gene="tsr"
                     /locus_tag="OQM83_002281"
                     /pseudo
     CDS             complement(10228..11883)
                     /gene="tsr"
                     /locus_tag="OQM83_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(12002..12448)
                     /gene="hpaR"
                     /locus_tag="OQM83_002282"
     CDS             complement(12002..12448)
                     /gene="hpaR"
                     /locus_tag="OQM83_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCS1520538.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            12720..14009
                     /locus_tag="OQM83_002283"
     CDS             12720..14009
                     /locus_tag="OQM83_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCS1520539.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            14006..15472
                     /gene="hpaE"
                     /locus_tag="OQM83_002284"
     CDS             14006..15472
                     /gene="hpaE"
                     /locus_tag="OQM83_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCS1520540.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15474..16325
                     /gene="hpaD"
                     /locus_tag="OQM83_002285"
     CDS             15474..16325
                     /gene="hpaD"
                     /locus_tag="OQM83_002285"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCS1520541.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16335..16715
                     /locus_tag="OQM83_002286"
     CDS             16335..16715
                     /locus_tag="OQM83_002286"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCS1520542.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            16826..17629
                     /gene="hpaH"
                     /locus_tag="OQM83_002287"
     CDS             16826..17629
                     /gene="hpaH"
                     /locus_tag="OQM83_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCS1520543.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17640..18428
                     /gene="hpaI"
                     /locus_tag="OQM83_002288"
     CDS             17640..18428
                     /gene="hpaI"
                     /locus_tag="OQM83_002288"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCS1520544.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            18603..19979
                     /gene="hpaX"
                     /locus_tag="OQM83_002289"
     CDS             18603..19979
                     /gene="hpaX"
                     /locus_tag="OQM83_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCS1520545.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            19989..>20231
                     /locus_tag="OQM83_002290"
     CDS             19989..>20231
                     /locus_tag="OQM83_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCS1520546.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         4870 a         5429 c         5168 g         4764 t
ORIGIN      
        1 tcttttaacc ctgcttgcat aacaggtatt ttactgataa caattccatt tttgcagcat
       61 cctggcaaca tatttcctcc acatccactg tctaaaaatc atgatggttt attcaggata
      121 ttcattcctt aaatgtttcc agcaaattaa tagattaagc gaataaatta tccttagcat
      181 tcactttcat atcagcatac tcctgctgca caacaactcc actgcatgtg tcagaaggtt
      241 agcatcaaat gataacctgg tgaaatagcc tggagacatt taaaatatct acattaagtt
      301 gttaggaata cttaatgaaa aacagaaatc acattcattt aatttataat taaatgatat
      361 cccttcccgc agaatgaaaa atcacacaac caatatttat tcaatgaaat ctgatggttt
      421 taacttgtta acattaaaaa acgtgcccgg ttatctatta tcctgattcg taaaggccaa
      481 acaataaatt cattatagtg ataatattca tgtgatagtt tgtttgcggc gagagataat
      541 tcgcttttta tcaccgagct tgtcccgata accaggatta atcagcgcaa ttttgatctt
      601 aacctaccgc aaaacaaccg tgcttcatga tacaaatgga ttctcattat ctgaacaata
      661 cgcagcacgt ctatgacaaa gggcgagtta tgcaaactga gcaacagcga gccgtaacac
      721 ggctttgtat ccagtgtgga ttatttcttt tgcaacatgg tgcggaaagc gcgttggttg
      781 atgagctttc ctcacgactg ggtcgggcgt tggggatgga cagcgtcgaa agttctatct
      841 cttcgaacgc catagtgctg acaaccatta aagatgggca atgcctgaca tcgacacgta
      901 aaaatcacga tcgcggcatt aatatgcatg tggtgactga agtccagcac attgtgattc
      961 tggcggagca ccatctgctg gattacaaag gcgtagagaa acgatttagc caaattcagc
     1021 cattacgtta cccaagatgg ctggtagcct taatggttgg cctttcttgc gcctgtttct
     1081 gcaaactcaa taaaggtggc tgggatggtg ccgtcatcac cttctttgcc agtacggccg
     1141 cgatgtatat ccgccagctg ctggcacaac gtcatcttca tccgcagatc aacttttgcc
     1201 tcaccgcttt cgccgccacc accatttccg gattgctttt gcaacttccc actttcagca
     1261 atacacccac tatcgcgatg gccgccagcg ttctgctgct ggtgccggga tttccgttga
     1321 ttaatgccgt cgccgatatg tttaaaggcc acatcaatac cggactggca cgctgggcga
     1381 tcgccagtct gctgacactg gctacctgcg tcggcgtggt gatggcactg acgatttggg
     1441 ggctacgcgg atgggtgtga tcgagtttct gtttgcattg gcgcaggata tgatcctcgc
     1501 cgccattcca gcggtcggct ttgcgatggt gttcaacgtt cccgttcggg cattacgctg
     1561 gtgtgcgctg cttggcgcga ttggtcatgg ttcacgaatg atcttaatga ccagcgggct
     1621 gaatattgag tggtcaacct ttatggcctc catgctggtc ggcaccattg gtattcaatg
     1681 gtcgcgctgg tatctggcgc atccgaaagt gtttaccgtg gcggccgtta tccctatgtt
     1741 cccgggcata tcggcttata ccgcaatgat ttcggcggta aaaatcagcc agttaggtta
     1801 cagcgaaccg ttgatgatta ccctgttaac caactttctt acagcttcat cgattgttgg
     1861 tgcgttatcc atcggtcttt ccattcctgg attatggttg taccgcaagc gccctcgcgt
     1921 ataaaattgc ctcgcttaaa gcacacggat gagagacagc ctcctctcct ccgtgtgtta
     1981 ctataaaagt tatctccctt ctcgttcatc gttccatatt tgagaaacag tatgtcttcc
     2041 agagttttga ccccggacgt cgttggtatt gacgccctgg tacacgatca ccaaaccgtt
     2101 ctggcaaaag ctgaaggcgg tgtggttgcc gtatttgcta acaatgcccc ggcgttttat
     2161 gccgtcacgc ctgcacgcct ggctgaactg ctggcgctgg aagaaaagct ggcgcgtccg
     2221 ggaagcgatg tcgctctgga cgatcaactc tatcaggaac cgcaagccgc tcccgttgct
     2281 gtacccatgg ggaaattcgc catgtatccg gactggcaac ccgatgccga ttttatccgc
     2341 ctggcggcgc tatggggtgt ggcactaaga gagccggtga ccaccgaaga actggcctca
     2401 ttcattgcct actggcaggc ggaaggtaaa gtctttcacc atgtgcagtg gcaacaaaaa
     2461 ctggcgcgca gcctgcaaat cggtcgtgcc agcaacggcg gactgccgaa acgagatgtg
     2521 aatacggtca gcgaacctga cagtcaaatt ccaccaggat tcagagggta acgatgaaaa
     2581 acgttggcga cctgatgcaa cgcctgcaaa aaatgatgcc tgcccatatc aaacctgcat
     2641 tcaaaacggg tgaagaacta ctggcctggc agaaagaaca aggagcgatc cgctccgccg
     2701 ctctcgaacg tgaaaatcgg gcgatgaaaa tgcagcgcac ctttaaccgc tccggtattc
     2761 ggccactgca tcagaactgc tcctttgaga actatcgcgt tgagtgtgaa gggcagatga
     2821 atgcgttaag caaagcgcgc cagtacgtcg aagagtttga cggcaacatc gccagcttca
     2881 tcttttctgg taagccagga accggcaaaa accatctggc ggcggcaatc tgcaacgagc
     2941 tgctgctacg cggtaaatcc gtattgatca tcaccgtggc cgatattatg tcggcgatga
     3001 aagatacctt caggaatagc ggtaccagtg aagaacaact gcttaacgat ctgagcaacg
     3061 ttgatctgct ggtgatcgat gagatcggcg tgcagaccga atcgaaatac gaaaaagtga
     3121 tcatcaacca gatcgtcgat cgccgctctt cttccaaacg cccaaccggg atgctgacca
     3181 acagtaatat ggaagagatg accaagttgc tgggcgaacg cgtgatggac cgtatgcgcc
     3241 tgggtaacag tttgtgggtg atcttcaact gggatagcta ccgtagccgg gtaacaggta
     3301 aagagtatta atccccgttt ctggcggtat actgcgacac aattccacct tgagtaatga
     3361 tgatgaaaac tgttaaacat cttctgtgct gtgccattgc cgccagcgca ttaatttcca
     3421 ccggggtgca tgctgcgtcc tggaaagatg cgctctccag cgccgccagc gaacttggca
     3481 accaaaacag cacgacacag gaaggcggtt ggtcgctcgc gtcattaact aacttgctta
     3541 gcagcgggaa ccaggcctta agcgcagata acatgaacaa cgccgcaggc attctgcaat
     3601 actgcgcgaa gcaaaagctg gcgtcggtaa ccgatgccga aaacatcaag aaccaggtgc
     3661 tggaaaagct gggcctgaac agtgaagagc aaaaagaaga caccaactat ctggatggca
     3721 ttcagggttt gctgaaaacc aaagatggtc agcaactcaa tctggataac atcggaacga
     3781 ctccgctggc agaaaaggtg aaaaccaaag cctgcgatct ggtgttaaag caggggctga
     3841 acttcatttc ctgaaccgcg aaacgccgcg ttttagctgt tacccggctg acgcggctct
     3901 ctcctgccct tttccctgct cctggacggt tttccccctg ttacacggga agcgaccgcg
     3961 atcaaaaaag tctgtttata aactaattcc taacaacatc gcattttttt gacactagaa
     4021 ttgcagcaat atggcgacgc cattacattg tgcttctcaa aaagcttaaa cgttggctaa
     4081 acggcccggt ttagtgagga tcgcccgttg tcagaactac tctctttcgc cctttttctc
     4141 gcctctgtgc tgatttacgc atggaaagcg ggacgtaaca cctggtggtt tgcagccaca
     4201 ttaacggtgc tggggctatt tgtcgtttta aatatcaccc tgtttgccag cgactatttt
     4261 actggcgatg gtattaacga cgcggttctc tataccttaa ccaacagcct gaccggtgct
     4321 ggcgtcagca aatacatcct gccgggtatc ggcattgtgc tggggctgac agcggtgttc
     4381 ggtgcgctgg gctggatcct gcgccgtcgt cgccatcatc cgcaccattt tggttacagc
     4441 ctgctggcgc tcttactggc gctgggttcg gtggacgcca gcccggcatt tcgtcagata
     4501 acggaactgg tgaaatccca gtcacgcgat ggcgacccgg actttgcggc ttattataaa
     4561 gagccgtcga aaactatccc tgacccgaaa ctcaacctgg tttatatcta cggcgaaagt
     4621 ctcgagcgga cctattttga taacgaagct ttcccggatc tcacgcctga actgggcgcg
     4681 ttgaaaaatg aaggcctgga tttcagccac acgcagcagc tgccaggaac ggattacacg
     4741 attgcgggca tggtggcttc tcagtgcggc ataccgctgt ttgccccctt tgaaggcaac
     4801 gcctccgcct ctgtctccag cttcttcccg cagaacatct gtctgggcga tatcctgaaa
     4861 aactcgggtt atcagaacta tttcgtgcag ggcgcgaatc tgcgttttgc cggtaaagat
     4921 gtgttcctga agtcgcatgg cttcgaccac ttatacggct cagaagagct gaaaagcgtg
     4981 gtggccgacc cgcactatcg caacgactgg ggattctacg acgataccgt tctcgatgaa
     5041 gcgtggaaaa agtttgaaga gctttcccgc tcaggtcagc gattctcact gtttaccctg
     5101 acagtcgata cccatcaccc ggatggtttt atctcgcgta cctgtaaccg caaaaaatat
     5161 gattttgacg gtaagccgaa ccagtcattc agcgcggtaa gttgcagcca ggagaatatc
     5221 gcggcgttta tcaacaaaat caaagcgtca ccgtggttta aagataccgt catcgtcgtc
     5281 tcttctgacc atttagcgat gaacaacacg gcgtggaaat acctcaataa gcaggatcgc
     5341 aataacctgt tttttgtcat tcgtggcgac aagccgcagc aagagacgct ggcagtgaag
     5401 cgtaacacga tggataacgg cgcgacggtg ctggacattc tcggtggcga taactatctc
     5461 ggacttggtc gtagcagttt atccgggcag tcgatgtcgg aaatcttcct caatatcaaa
     5521 gagaaaacat tggcgtggaa gccggatatc atccgcctgt ggaaattccc taaagagatg
     5581 aaagagttca ccatcgacca gcagaaaaac atgattgcct tctcgggtag ccatttccgt
     5641 ttgccgctgc tgttgcgggt ttcagacaaa cgcgtggaac cgctgccgga aagcgaatac
     5701 tcagcaccgc tgcgtttcca gctggccgat ttcgctccac gcgacaattt cgtctgggtt
     5761 gaccgttgct ataagatggc acaactctgg gctccggaac tggcactctc caccgactgg
     5821 tgtgtctcgc aagggcagct tggcggtcag caaattgttc agcatgttga caaaacaaca
     5881 tggcagggca aaacggcatt taaagatacg gtcatcgaca tggcgcgtta taaaggcaat
     5941 gtcgatacgc tgaaaattgt tgataacgat attcgctaca aagccgacag tttcatcttc
     6001 aacgtcgccg gtgcgccaga agaggtgaaa cagtttagtg ggatttcccg tccagagtcg
     6061 tggggccgct ggtccaacgc gcagctgggc gatgaagtaa aaatcgagta caagcatccg
     6121 ctgccaaaga aatttgacct ggtgattacc gccaaagcat acggcaataa cgccagccgt
     6181 cctattccgg tacgcgtagg caatgaagaa caaacccttg tgctgggcaa tgaagtgacc
     6241 accaccacac tgcatttcga taacccaacc gatgccgaca cactggtaat tgtgccgccg
     6301 gaacctgtct caaccaacga agggaatatc ctcggacact cgccgcgtaa gctcgggatc
     6361 ggcatggtgg aaattaaagt ggtggaacgt gaaggataat tcctgactga tgtgtaggtc
     6421 gcataaggtg cgacctacac accttctgcg gcctacaaaa catcattgag aaacagaaaa
     6481 aaacagcgcc caacacttcc ccggacgctg catggcaact ttaataatct cgccttatca
     6541 gaaagtaatt acacccttaa tcaactcacg attgttaatc acatcgcgct cgtaggtttc
     6601 tgccagcgtg gcgaacggat agcgatgggt taacatcatg tcagcagtga tttttccttc
     6661 cgccatcagt cgaccgactt tagcaaaatc ttccggcgtg gcgttgcggc tacccatcat
     6721 cgtcgtttct tttttatgga attccggatc ggagaactgc aactcacctt taaacaggcc
     6781 gacaaatacc accgtgccgc cgtgacgaat caaattcacg gtgttattca tcgcatgttg
     6841 attacctgtc gcgtcgatca ctttctgcgc cagcgaacca ccaaactgcg cccgtagctg
     6901 cgcgtcaaaa tcttcggctg acgggtccag taaaggtaat tccagacgcg ttgccacatg
     6961 ttcacggcgc gccggactgg tatccgccac caccacctgt gcgccatcgg ctttagcgat
     7021 tgccgccgcg cccagaccga ttggccccgc cccgaccacc agcacctgct cgccgggagc
     7081 aatggctgcg cgacgcaccg catgagcgct aatagcgaaa ggttcaatca atgctgccgc
     7141 ctgcgggtca ataccgtctg cgggcaaaat gttcgccacc ggcacgctca aatactcact
     7201 aaaaccgcca tcctgatgca cgccaatgac tgaaattttt tcacagcaat tggtacgccc
     7261 gcttttacac gccgggcatt gctgacaggc aacataaggg atcacagcaa cttgctgacc
     7321 atttttaaga tcagcaatat ttttacccag cccaacaatc tccccacata tttcatggcc
     7381 taaaacacgt ggataactaa aaaatggttg atttccaccc caggcatgaa tatcggtacc
     7441 gcaaatcccg acagacttaa tttttattaa tgcttcattg tcacccggaa taggtatctc
     7501 gcgttgtttc cagactaatt ctttcggctg ctggcaaatt aaaacattca tcgtagacat
     7561 gattcggctc tccatttttt gttgccttag atatcaatga aaatgaaaaa tggcagcgga
     7621 acggaaaatc tgttttgtga aaacacacgc ataaaaatgt tttaaatcgg gttttaatgg
     7681 actcacaagg aggaaaatat gagtcgttca caaaatttac gccacaatgt gattaaccag
     7741 gtcattgatg atatggcgcg gggtcatatc ccttccccac ttccatcgca aagtgcgctg
     7801 gcggaaatgt acaacatcag ccgcaccaca gtgcgtcata ttctcagcca cttacgcgaa
     7861 tgcggcgtct tgacgcaggt tggaaacgac tatgttattg cgcgtaaacc tgaccatgat
     7921 gacggtttcg cctgtactac cgcttcaatg agtgaacaaa acaaagtgtt tgagcaggct
     7981 tttttcacga tgataaacca gcgccagtta cgcccagggg aaacgttttc tgaactgcaa
     8041 ctggctcggg ccgcgggtgt cagcccggtt gtagtaagag aatatctttt aaaattcggc
     8101 cgttataatc ttattcacag cgaaaagcgc ggccagtgga gcatgaagca attcgatcaa
     8161 tcctacgccg agcagctgtt tgagctacgc gaaatgctcg aaacacattc acttcagcac
     8221 tttctcaacc tgcctgatca tgatccccgc tggctgcagg cgaaaaccat gctcgaacgt
     8281 catcggctac tgcgtgacaa catcggcaat agctttcgca tgttctcgca acttgacaga
     8341 gattttcact ccttactgct ttcagctgcc gacaatatct tttttgatca atcgcttgag
     8401 atcatctcag tcatctttca ttttcactat caatgggacg aaagcgatct taaacagcgc
     8461 aatatcattg ccgtcgatga gcatatgacc attctcagcg ctctgatttg ccgcagtgac
     8521 cttgatgcca cccttgcgct ccgtaaccat ttgaattcag ctaaacaatc aatgattcga
     8581 tccattaatg agaacacgcg ttatgctcat taagtacgga ttaaaaacag caaactatta
     8641 gcgggtgcac ggttttatca aaagataaat accgggaaat tcccgcttac ctatgctcac
     8701 aatcagtcga ctaaaaatgt ttttgtgagc ggtagtaaag tcctaaaact ttaacctgcc
     8761 gttaatgtca accttgcgta attcacaatt acgtaaacaa ttaaaattat aaatacagga
     8821 gtccggcgtg gaaaaagaaa atatcaccct cgatccgcgt tcttcattta ctccatcttc
     8881 gtcggcagat attcccgtgc caccagatgg attagttcaa cgcagtaccc gaattaaacg
     8941 cattcaaacc accgccatgt tgttattatt ttttgcggcg gtaatcaatt atctcgaccg
     9001 cagttcgctg tcggtagcaa atttaacgat tcgtgaagaa ttgggattaa gtgccaccga
     9061 aatcggcgct ttgctctccg tgttttcact cgcttacggg attgcgcaac ttccttgcgg
     9121 cccactattg gatcgtaaag gcccacgcct gatgctggga ctggggatgt tcttctggtc
     9181 actgttccag gcaatgtctg gcatggtgca caactttacg cagttcgtgt tggtgcgtat
     9241 cggtatgggg attggtgaag cgccgatgaa cccatgcggt gtaaaagtca ttaacgactg
     9301 gttcaacatc aaagagcgcg gacgcccgat gggcttcttc aacgcagctt ctaccattgg
     9361 cgttgccgta agcccaccga ttctggcggc gatgatgctg gtgatgggct ggcgcgggat
     9421 gtttattacc attggtgtac tggggatttt tctcgccatc ggctggtata tgctctatcg
     9481 caaccgcgag cacgtagaac tgactgccgt tgaacaagct tatctcaatg caggtagcgt
     9541 caatgcccgc cgagatccgc tcagttttgc cgaatggcgc agcctgttcc gtaaccgtac
     9601 aatgtgggga atgatgctcg gattcagtgg catcaactac actgcgtggc tgtatctggc
     9661 ctggcttcct ggttacctgc aaacagccta taacctggat ttaaaaagca cagggttgat
     9721 ggcggctatc cctttcctgt ttggggctgc cgggatgctg gtcaacggtt acgttactga
     9781 ctggctggtc aaagggggaa tggctccgat taaaagccgt aagatctgca ttattgccgg
     9841 gatgttctgt tctgccgcct ttacgctggt cgtaccgcaa gcgacaacat ccatgacagc
     9901 ggttctgctg attggcatgg cactgttctg tattcaacat cctgctgggg cttgatccac
     9961 gtcgcagttg cttctcgcat gactgcgtcg gtgggcagta tccagaactt tgccagcttc
    10021 atctgcgcct cttttgcgcc gatcattact ggttttattg ttgataccac ccactcattc
    10081 cgtctggcac taatcatctg cggttgcgtc accgcagcgg gggcactggc gtacatcttc
    10141 ctggttcgtc agccgatcaa cgacccacgt aaagattaat ctccttatgc ccgataacat
    10201 tttgcttatc gggcattttc atggcgatta aaatgtttcc cagttctcct cgctatctgc
    10261 cacggccatt ttacgcggcg cagctggcgt cacggttttt accacagccg atgtttcacg
    10321 ctgctgttgc tgaatccgga acactgccac tgcttcggtc aggcgactgg cctgctcttc
    10381 cagcgcggcg gcggcagcgg cagactcttc caccagcgcg gcgttctgtt gagttacccg
    10441 gtccatctca gcaaccgcta agccaacctg atcgatacca cggctctgct catcagaagc
    10501 agaagcaatt tcgcccataa tgtccgtcac gcgggtcacg gcgctgacaa tctccgccat
    10561 tgtttccccg gcgctttcga ccagcgtaga gccaacatcc actttcccca ccgagtcttc
    10621 aatcaggctt ttaatttcac gagccgcctg ggcgctgcgc tgggccagat tacgcacttc
    10681 tcccgcgacc accgcaaaac cgcgcccttg ctcacccgca cgcgcagcct caaccgccgc
    10741 gttcaaagcc agaatattgg tctggaaggc aatgccgtca attacgctga taatatcggc
    10801 gattttctgc gaactggtgg agatatcgcg catagtctgc accacgttat ctaccacttt
    10861 gccgccgcgt tgcgccgttt cagaagcact taacgccaga tggctggcct ggcgcgcatt
    10921 ttcggcgttc tgcttcaccg ttgcggtcag ttactccatg ctggctgccg tctcttccag
    10981 cgaagcggcc tgttgctcgg tgcgcgaaga gagatcgtta ttgccggtgg cgatttcgct
    11041 ggcaccgcta tagatggcat tggccccgtt gcgcacatca ccgacggtac gcatcagctc
    11101 tccctgcata tggcgcaaac tctctgccag ttgccccatc tcattagagc catccacctc
    11161 aatcggtttc accagatcgc cgcctgcaat atgacgaatg ctgtcaatca ggcgattcat
    11221 tggcgctacc agcgaggctt taataccgaa ccagacggcg aagatgaccg ccagtacgac
    11281 gatcatcacg cccaccagaa tccacatcgc ctggctgtag gaggcattgt tatcgctgac
    11341 ggcgatatca tagagccgat cgttttgctc catgtaagcc acatactgct tctcgaaacc
    11401 gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg attttgcctg cacctaacag
    11461 ttggatcagc tccgccagcg cattgtgata aatatcgtaa ttacgtttga tctctgccgc
    11521 tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc agtttttttc
    11581 cgcctgtttc agcgaaatac tggcactctg catcagctca gcaacggttg aaccgctacc
    11641 aatattgttc tgatccatca tgtagcggat acccgcgcgg ttgagggtgt tacgcgtctg
    11701 caacaacgcg acccagctgc cattcagcgt ggattgctgc tggcgaatgg tttgtaaaac
    11761 agtgaaattt tctttgtcat tctttaaggc attaaagaac agaccgcctg atgtcagttg
    11821 taaaaggcca aaaacggcca aaaccagcag taagctggtc acgatcttga tacgttttaa
    11881 catggtttct ctttcctgtg gaccgatgca agattttcgg cctggaaagc aaaaacttta
    11941 tgaaaaggtg taacccacca tccatggaaa aaccaggcgc tggttatcag atactaaaaa
    12001 gttattcatt atcgcccggg atggcttctt gtcgggaatt gcccagagca ataaattcct
    12061 ctaacagatg ggttaactgt tgcattttct cggcagtaaa ctgagcttca atctgccgat
    12121 aggcttcttc aatctgtgtc tgagcgcggt tatacagcgc ctgcccctct ttggttagcg
    12181 agatatagag cttccgctga tcattgatcg gctttaatcg caacactaaa ccgtcgcgtt
    12241 ccatccgcgt gaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga tacgccagat
    12301 cgtgaaaatc cattgatggg ctttccgcca gaatacgcac gatgcgccac tgttgctcag
    12361 tcaaattatg ccgtttaacg attgggcgaa aataactcat cgccgcttct cgcgcctgga
    12421 gcaacgcaat ggttagtgag tcgtgcatta tctttcccct gaaaaattca aaatcattaa
    12481 tatagaaaca gttaatcgtc acgttaccgg gactgcgctg cccctgcaat tcctgatttt
    12541 atcaaaattg atgatatttt attaacttac tgatatttaa tattttatta tctttaaatg
    12601 taaatagttt gttaattaga tcacatttac atcacttact tttgccaaac tacgcaatcg
    12661 ggaataaaag caatcattaa catattaatg attaagatga tcgaatccga ggagtggtaa
    12721 tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccaacttgat gcatggcagg
    12781 aagcgttcca gcaatccccc tacaaagccc cgcctaaaac tgcggtctgg tttattaaac
    12841 cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt gaaaaggtac
    12901 tgagcggtgc gactgttgcg ctgattgtgg gaaaaacggc gacgaaagta cgtgaagaag
    12961 atgcggcaga gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg ccggaagaga
    13021 gcttttaccg cccggcaatc aaagcaaaat gtcgtgatgg attctgcccc attggcgaaa
    13081 ccgtggctct cagcaatgtc gataatctga ccatctatac cgagatcaac gggcgtcctg
    13141 ccgatcactg gaataccgcc gatttacaac gtaacgccgc gcagttgctg agcgccctga
    13201 gcgaatttgc cacgctgaat ccaggcgacg ccattctgct cggcacgcca caggcgcgcg
    13261 tggaaataca gccaggcgat cgcgttcgtg ttctcgcaga aggtttcccg ccgctggaaa
    13321 atccggtagt ggacgaacgt gaagtgacca cgcacaagag cttcccaacg cagccacacc
    13381 cgcacggcac gctgtttgcc ctcggcctga actacgccga ccacgccagc gaactggaat
    13441 ttaagccacc ggaagaaccg ctggtgttcc tgaaagcgcc aaataccctc actggcgata
    13501 accagacctc cgtgcgtcca aacaatattg aatacatgca ctatgaagcg gagctggtgg
    13561 tagttattgg caaacaggcg cgtaacgtca gcgaagccga tgccatggat tatgtcgcgg
    13621 gctacaccgt gtgtaacgac tacgccattc gtgactatct ggaaaactac taccgcccta
    13681 acctgcgggt aaaaagccgc gacggactga cgccgatgct ttcaaccatc gtgccgaaag
    13741 aggcgatccc ggacccgcat aatctgaccc ttcgcacctt cgtcaacggc gagttacgcc
    13801 agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc tacttaagtg
    13861 aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacacccaaa ggcttatctg
    13921 acgtggtgcc tggcgatgaa gtagtggtgg aagtagaagg cgtgggccgc ctggtgaacc
    13981 gaattgtgag tgaggataca gcgaaatgaa aaaagtaaat cattggatca acggcaaaaa
    14041 tgttgcaggt aacgactact tccagaccac caatccggca acgggtgaag tgctggcgga
    14101 tgtggcctct ggcggtgaag cggagatcaa tcaggcggta gcggcagcga aagaggcgtt
    14161 cccgaaatgg gccaatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc gtctgggcga
    14221 tctgatcgac cagaacgtgc cagagatcgc cgcgatggaa accgcggaca ccggcctgcc
    14281 gatccatcag accaaaaatg tgttgatccc acgcgcttcc cacaactttg aatttttcgc
    14341 ggaagtctgc cagcagatga acggcaagac ctatccggtt gacgacaaga tgctcaacta
    14401 cacgctggtg cagccggtgg gcgtttgtgc gctggtatcg ccgtggaacg taccgtttat
    14461 gaccgccaca tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg tactgaaaat
    14521 gtcggaactc tccccgctga ccgctgacag cctgggtgag ctggcgctgg aagccgatat
    14581 tccggcaggc gtgctgaacg tggtacaggg ctacggcgca accgcagggg atgcgctggt
    14641 tcgtcatcat gacgtacgtg ccgtgtcgtt caccggcggt acggccaccg ggcgcaacat
    14701 catgaaaaac gccgggctga aaaaatactc catggaactg ggcggtaaat cgccggtgct
    14761 gatttttgaa gatgccgata ttgaacgcgc gctggacgcc gccctgttca ccatcttctc
    14821 gatcaacggc gaacgctgca ccgccggttc gcgcatcttt attcagcaaa gcatctaccc
    14881 ggaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtgggcg atccgaacga
    14941 tccgaatacc caggttggcg cgcttattag ccagcagcac tgggaaaaag tctccggcta
    15001 tatccgtctc ggcattgaag aaggcgcaac cctgctggcg ggcggcccgg ataaaccgtc
    15061 cgacctgcct gcgcacctga aaggcggcaa cttcctgcgc ccaaccgtgc tggcagacgt
    15121 tgataaccgt atgcgagtcg cccaggaaga gattttcggg ccggtcgcct gcctgctgcc
    15181 gtttaaagac gaagccgaag gcttacgtct ggcaaacgac gtggagtacg gcctcgcgtc
    15241 gtacatctgg acacaggatg tcagcaaagt gttacgcctg gcgcgtggca ttgaagctgg
    15301 catggtgttc gtcaacaccc agaacgtgcg tgacctgcgc cagccatttg gcggcgtaaa
    15361 agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg cggaaatgaa
    15421 gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct gatatgggta
    15481 agttagcgtt agccgccaag atcacgcacg taccgtcgat gtatcactct gaactgccag
    15541 ggaaaaacca cggttgccgc cagggcgcga tcgacgggca taaagagatc agcaagcgtt
    15601 gccgggaaat gggcgtcgat accattatcg ttttcgatac ccactggctg gtcaacagtg
    15661 cttatcacat caactgtgca gaccattttg aaggcgtcta caccagcaac gagctgccgc
    15721 attttatccg cgatatgacc tacaactacg agggcaaccc ggagttgggg cagcttattg
    15781 ccgatgaagc cttaaagctc ggcgtgcggg caaaagcgca caacattccc agcctgaaac
    15841 tggaatacgg cacgctggtg ccgatgcgct acatgaatga agacaagcac ttcaaagtgg
    15901 tctccatttc ggctttctgc acggttcacg attttgccga cagccgcaag ctgggcgaag
    15961 cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc agcggttcgt
    16021 tatcgcaccg ctttattgac gatcagcgtg cggaagaagg gatgaacagc tacacccgcg
    16081 agttcgaccg ccagatggac gagcgcgtgg tgaagttgtg gcgcgaaggc cagttcaaag
    16141 agttctgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc aatatgcacg
    16201 acacggtgat gctgctgggg atgctcggct gggataaata cgacggcaag gtggagttta
    16261 tcaccgagct attcccaagc tctggcaccg gtcaggttaa cgctgttttc ccgcttcccg
    16321 cgtaaggacg ttttatgccg cactttatcg ttaaatgcag tgacaacatc cgcgaagaag
    16381 ccgacctgcc ggggttgttc gccaaagtga atccgacgct ggcagccacg ggtatttttc
    16441 cgctggcggg tattcgcagc cgcgtgcatt gggtcgatac ctggcagatg gccgacgggc
    16501 agcgtgatta tgccttcgtg catatgacgt tgaaaatcgg cgcaggtcgc agcctggaaa
    16561 gccgccagca ggcgggtgaa atgctgtttg aactgattaa aacgcacttc gccgccctga
    16621 tggagagccg cctgctggcg ttgtcgtttg agattgaaga gctgcatccg acgctgaatt
    16681 ttaaacaaaa caacgtgcac gcattgttta agtagcgcgc agattgcccg gtggcgctgc
    16741 gcttaccggg cctacaaaac cccaaaccgt acaccgtagg ccggataagg cgcagccgca
    16801 tccggcaatg ccacaggata tcgctatgtt cgataaacac acccacaccc tgatcgccca
    16861 gcgtctggat caggcagaaa aacagcgcga acagatccgc gcgatctcgc tggattaccc
    16921 ggagatcacc atcgaagacg cttacgcggt gcagcgtgaa tgggttcgcc tgaaaatcgc
    16981 cgaaggtcgc acgctgaaag gccacaaaat cggcctgacc tcgaaagcga tgcaggcaag
    17041 ctcgcagatc agcgaaccgg attacggcgc gctgctggat gacatgttct tccacgatgg
    17101 cagcgatatc ccgaccgatc gctttatcgt gccgcgcatt gaagtggagc tggctttcgt
    17161 gctggcaaaa ccgctgcgtg ggccaaactg cacgctgttc gacgtctaca acgccacgga
    17221 ctacgtgatc ccggcgctgg agctgatcga cgctcgctgc cacaacatcg atccggaaac
    17281 ccagcgcccg cgcaaagtgt tcgacaccat ttctgataac gccgccaacg ccggggtgat
    17341 cctcggcggt cgtccgatca aaccagacga actggatctg cgttggatct ccgccctgat
    17401 gtatcgcaat ggcgtgattg aagaaaccgg cgtcgccgct ggcgtgctga atcatccggc
    17461 aaacggcgtg gcctggctgg cgaacaaact cgcaccgtat gacgtacaac tggaagccgg
    17521 gcaaatcatt ctcggtggtt cgttcacccg cccggttccg gcgcgtaagg gcgacacctt
    17581 ccacgtcgat tacggcaaca tgggttccat tagctgccgc tttgtttaag gagagaacga
    17641 tggaaaacag ctttaaagcg gcgctgaaag caggccgtcc gcagatcgga ttatggctgg
    17701 ggctgagtag cagctacagc gcagagttac tggccggagc aggattcgac tggttgttga
    17761 tcgacggtga gcacacgccg aataacgtgc aaaccgtgct cacccagcta caggcgattg
    17821 cgccctaccc cagccagccg gtagtacgtc cgtcgtggaa cgatccggtg caaatcaaac
    17881 aactgctgga cgtcggcaca caaaccttgc tggtgccgat ggtacaaaac gctgacgaag
    17941 cccgtgaagc ggtacgcgcc acccgttatc cccccgccgg tattcgcggt gtgggcagtg
    18001 cgctggcccg cgcctcgcgc tggaatcgta ttcctgatta cctgcaaaaa gccaacgatc
    18061 aaatgtgcgt gctggtgcag atcgaaacgc gtgaggcaat gaagaactta ccgcagattc
    18121 tggacgtgga aggcgtcgac ggcgtgttta tcggcccggc ggatctgagc gccgatatgg
    18181 gttatgccgg taatccgcag cacccggaag tacaggccgc cattgagcag gcgatcgtgc
    18241 agatccgcga agcgggcaaa gcgccgggga tcctgatcgc caatgagcaa ctggcaaaac
    18301 gctatctgga actgggcgcg ctgtttgtcg ccgtcggcgt tgacaccacc ctgctcgccc
    18361 gcgccgccga agcgctggca gcacggtttg gtgcgcaggc caccgccgtg aagcccggcg
    18421 tgtattaatg ccggatgcgc tgcgcttatc cggcctacac tcgcaccaaa ccgtaggccg
    18481 gataaggcgt ttacgccgca tccggcaaaa agccgtacca aatcgcggac cggataaggc
    18541 gttacgccgc atccgccata aaccttgtcg taccctacaa aaatcccatt agaggaagaa
    18601 aaatgagcga cacctcacct gccataccgg agagtatcga tccggcgaat cagcataaag
    18661 cgctgactgc cggacaacag gcggttatta agaagctatt tcgccgcctg agcgtctttc
    18721 tgttcgtgct gtttatcttc tcgttccttg atcgcatcaa catcggcttt gccggactca
    18781 cgatgggacg cgacctcggt ctgagcgcca ccatgtttgg cctcgctacc accctgttct
    18841 atgccgctta tgtcatcttc ggcattccca gcaacattat gctgagtatt gtcggtgcgc
    18901 ggcgctggat cgccaccatc atggtgctct ggggcatcgc ctctactgcc accatgtttg
    18961 ccactggccc caccagcttg tacgtactgc gtatactggt tggcattacc gaagccggct
    19021 ttctgcctgg tattctgctg tatttaacct tctggtttcc ggcctatttc cgcgcccgtg
    19081 ccaacgcctt gtttatggta gcaatgccgg taacgacagc gttgggctcg atcgtttccg
    19141 gctacatttt gtcgctggat ggcgtcatgg cattaaaagg ctggcagtgg ctgtttttgc
    19201 tggaaggctt cccgtcggta ttactcggcg tcatggtgtg gttctggctt gatgactcac
    19261 cggacaaagc taagtggctg acgaaagaag acaaaaaatg cctgcaagag atgatggata
    19321 acgatcgtct gacgctggtt cagccagagg gagccatcag ccaccacgcc atgcaacaac
    19381 gcagtatgtg gcgggagatc ttcactccgg tggtgatgat gtataccctg gcgtatttct
    19441 gcctgaccaa cacacttagt gcgatcagca tctggacacc gcagatcctg caaagtttta
    19501 atcagggcag cagtaatatc accatcggca tgctggccgc cgtaccgcag atttgtacca
    19561 ttctcgggat ggtctactgg agccgtcact cagatcgccg ccaggaacga aggcatcaca
    19621 ccgcccttcc ttatttgttc gctgccgcag gttggttact ggcttcggca actgatcaca
    19681 acatgatcca gatgctgggg atcattatgg cttcgaccgg atcattcagc gcaatggcga
    19741 ttttctggac aacaccggat cagtccatca gcctgcgggc acgagcgatc ggtattgcgg
    19801 tgatcaacgc cactggcaac attggttcag cgttaagtcc gtttatgatc ggctggttga
    19861 aagatttgac cggcagcttt aacagtggat tgtggtttgt tgccgcgctg ctggtgattg
    19921 gtgcggggat tatctgggcg attccaatgc agtcctcccg tccgcgagcg accctgtaag
    19981 gaacgacgat gtgtgaccgt cagattgcca atattgatat cagcaaagag tacgatgaaa
    20041 gcctgggcac ggacgatgtg cattatcagt cgttcgcccg catggcggca ttttttggcc
    20101 gccatatgct gccacatcgc cacgaacagt actttcagat gcatttcctc aatagcggac
    20161 agattgagct acagcttgac gatcatcgct actcggtgga agcgcccctg tttgtcctga
    20221 cgccgccgtc a
//
LOCUS       DAJVQG010000072        20147 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJVQG010000072 DAJVQG010000000
VERSION     DAJVQG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20147
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.072"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..1787)
                     /gene="ygfK"
                     /locus_tag="OQM83_002291"
     CDS             complement(<1..1787)
                     /gene="ygfK"
                     /locus_tag="OQM83_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HCS1520547.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSIGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVPLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQ"
     gene            complement(2109..2687)
                     /gene="mocA"
                     /locus_tag="OQM83_002292"
     CDS             complement(2109..2687)
                     /gene="mocA"
                     /locus_tag="OQM83_002292"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HCS1520548.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            2790..3560
                     /gene="yqeC"
                     /locus_tag="OQM83_002293"
     CDS             2790..3560
                     /gene="yqeC"
                     /locus_tag="OQM83_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HCS1520549.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            3608..5233
                     /locus_tag="OQM83_002294"
     CDS             3608..5233
                     /locus_tag="OQM83_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HCS1520550.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILWVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(5274..6206)
                     /gene="arcC"
                     /locus_tag="OQM83_002295"
     CDS             complement(5274..6206)
                     /gene="arcC"
                     /locus_tag="OQM83_002295"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCS1520551.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(6254..7638)
                     /gene="hyuA"
                     /locus_tag="OQM83_002296"
                     /pseudo
     CDS             complement(6254..7638)
                     /gene="hyuA"
                     /locus_tag="OQM83_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
     gene            complement(7691..8902)
                     /locus_tag="OQM83_002297"
     CDS             complement(7691..8902)
                     /locus_tag="OQM83_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HCS1520552.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(8960..10156)
                     /gene="dpaL"
                     /locus_tag="OQM83_002298"
     CDS             complement(8960..10156)
                     /gene="dpaL"
                     /locus_tag="OQM83_002298"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HCS1520553.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(10214..11401)
                     /gene="ygeW"
                     /locus_tag="OQM83_002299"
     CDS             complement(10214..11401)
                     /gene="ygeW"
                     /locus_tag="OQM83_002299"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCS1520554.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            11880..13658
                     /locus_tag="OQM83_002300"
     CDS             11880..13658
                     /locus_tag="OQM83_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HCS1520555.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(13698..14177)
                     /gene="xdhC"
                     /locus_tag="OQM83_002301"
     CDS             complement(13698..14177)
                     /gene="xdhC"
                     /locus_tag="OQM83_002301"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCS1520556.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(14174..15052)
                     /gene="xdhB"
                     /locus_tag="OQM83_002302"
     CDS             complement(14174..15052)
                     /gene="xdhB"
                     /locus_tag="OQM83_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCS1520557.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(15063..17360)
                     /gene="xdhA"
                     /locus_tag="OQM83_002303"
     CDS             complement(15063..17360)
                     /gene="xdhA"
                     /locus_tag="OQM83_002303"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCS1520558.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            17775..18530
                     /locus_tag="OQM83_002304"
     CDS             17775..18530
                     /locus_tag="OQM83_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCS1520559.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            18609..18678
                     /locus_tag="OQM83_002305"
     tRNA            18609..18678
                     /locus_tag="OQM83_002305"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18641..18643,aa:Gly,seq:ccc)
     gene            18871..>20147
                     /locus_tag="OQM83_002306"
     CDS             18871..>20147
                     /locus_tag="OQM83_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1520560.1"
                     /translation="MEYFMKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYM
                     STLDQIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQND
                     ALGLYLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDAR
                     LNTSSVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYE
                     RITLQLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAG
                     PYGIKRYEKDNYQSANFWFNDIKTDTDQNSHAK"
BASE COUNT         4980 a         4765 c         5180 g         5222 t
ORIGIN      
        1 aactgaggaa tgatattttt caccacgccg ccagcattgg cttcgcgttc aaacagcgta
       61 accggatggc ccgctctggc aaggaagtaa cctgctgcca gacccgccgg acctgcacca
      121 atcacggcaa ccggatggcg tgaaccagaa ccggcgggtt tgtgccagcg ttgcttatat
      181 tcatcccaac ctttttccag cgcgactttt ttcagttcgc ggatattcag cgcactgtcg
      241 taatccaggc gggtacagtt gtattggcac tggtgatcgc aaatatgacc ggtaatggca
      301 ggtagggcgt tgcgttggta gatgagttcc agcgcgtcgg catagcggtg ttcgccaagc
      361 agacggatgt attccggaat atcttgcttg atagcgcagg cagtaacaca gggggcaacg
      421 tagcaatcgg tcagcggcag gtcttctgcc acttcaatac gctcttctgg cttccagtgt
      481 ttctgggtgt attccatggt taacgcgtct gctgccagtc tgttcagtcg ttcgacgtca
      541 acatggtcaa gtccccaggc gtcggagcct tccagctcgc gcatgcaggc acttaagcgc
      601 agatagccgc caggtttcag caggtcggtt gccatagtaa tagggcgaat acctgtatca
      661 aaaatatcgc ggatagtcag ctgactggca ccaccggaat aagaaatggg cagtttgccg
      721 tcaaaggcgc gagagagaac tgctgcaaca ttgatggaga gcgggaacag cgcacggcct
      781 gacatataca tctcttcacc aggcagtgcg cctttattgt tgatggtgcc gagagtgtta
      841 gtcagtttta cgccaaagcc gagtgatttt tcttttgcca gtaccatcag gcgctccagc
      901 atttccagtg cttgcgtcag cttgaggtcg tgatcaaatg actcttcttt taagcctatg
      961 taaccgaaac cgcagacatc gaggatctca cgaacacgcg cgtaccccag taaggtcggg
     1021 ttaagtttga caaaggtgtt gagccctttt tcttccagca tgtagcggca aatggcttcg
     1081 atttcatgcg gaggacagcc gtgcatggtg gagaggggga cgccctgcac catactggtg
     1141 gggatgcgag cgggtaaggc ttgcaagttt tcgcgttttt cctgcaatcc gtggcgggct
     1201 aaaaatgcgt catcctggag taatttattc agcgtatcgc gatattgagc gaatttcgga
     1261 tggtcagatg cgtccatcat attgtcgatg aactgttgca tcggcggttg cttaatacct
     1321 tcgaggttgt aaccgatgct catattaaag atgaacgatt taccggaatc agaaggctgt
     1381 aacatcgctt cgagaaggtg cagggcaaac caggctttga ggtattcatc ccaggcttta
     1441 agcaaggtaa attcggtaga ccattcggtg ttaaagcatt cgtcttcggc atcaatacag
     1501 ggcttttcca gctccaggcg gtcaagaatt tggacggttt ttagttcgat gaagcgtccg
     1561 ccagtcagcc aggaagtgac aatattttgc gcgagctgcg tgtgcgggcc agcggcaggg
     1621 ccgacgggag tggcacaggt ttcaccgaag acgctaacag ttttaccttt tacaggtgag
     1681 taaaactgtt gctcgggaat accaaagatt gagcgttgtt gttggtattc atcaaatatg
     1741 cgcgtcaaaa gttcctcaaa cggaatggga cgcataatat cccccataac cctctcctta
     1801 gcttatacag atatcattga gtgaccggtg ttgagaaatc cggtggttta gggggataga
     1861 gcaacaatcg caccactctt gccaggtttg gcgggaaata taaaaaaatg tgaggtggct
     1921 cgaaacttaa aggctttttg tgaatgaact cacaggtgcc aaatgagaat taaaatcact
     1981 cctgaaaatt attattgtca gcccatttat cactttttct cttttctcaa aatcagtgat
     2041 tccttatcat tatgataaag agtgctggag agattaaata tttgaacgca atcgtttaac
     2101 tacattttct aaattttagt ataccttttt tgcgctgtaa taaagtcgtc cggagtatca
     2161 atatctaaaa ttatttctgc attttctatt tcgacggaat aatgatctcc cataaggagt
     2221 gcctgacgca tattggtgac attgggtcgc tggattgcct gcatcaggca tggttttgac
     2281 actaaaatcg gatggccggg gatgccatta tggagtggca gtattgcgcc atcatttcgt
     2341 aacgaccaga tttttctaaa aatatcgatg gtgagggttg gcatgtcacc gtgggtgaga
     2401 aaacaatgtt ctgtttgcac tgcgggtact gcggccttga cggatgttag taaaccctgc
     2461 gcataatctg ggttgtgaat aatagtgata ttgctctggt tcgcataacg ttcgtgcagt
     2521 tcattaccac gatagccggt gactaaaata attcggctac aaaactgcaa cgcatttttg
     2581 atacttgtat caagaattgt tccctgttcc caggggaaca tcattttcca ttgccccatt
     2641 cttgatgata atccggcagc ggtaattata cagtcgatgg ctgacatagt ggttccatta
     2701 tttatagggg ctaataacgc ctgataattg cgttacttcc tgatggatta agttggggat
     2761 aatttatccc agagaggtca taaagactcg tgaaaagtat agttgaccca tcggcattag
     2821 tcattgattt aggtgcgcag aaacgtccca ctgtgatttc tgttgttggt gctgggggga
     2881 aaaccagctt gcttttttgg ctggcagaac tgctccaggc tagcggcagg cgtgtgttaa
     2941 tcacgacgac gacacatatg tttatgccaa cgtctcactg gcccgtggtt ttctgtcgtg
     3001 atcccgccat gcttcctcat gcgtctctta catcccccat ttcattttgt tttcacagct
     3061 ggaaagcgaa ccagggaaaa gtgcagggat ttacgccaga agcgattgat gcactggtgc
     3121 aacgaccaga gtgtgacgta attctcattg aagcagatgg ctcgcgtgga atgccgttaa
     3181 aagcgcctga tgagcacgaa ccttgcatac ccaaaagcag ttgctgcgtg attgctgtga
     3241 tgggaggaca tactttgggc gcgaaagtga gcacagaaaa tgtccatcgc tggtcgcagt
     3301 ttgctgatat taccgggtta acacctgatg caaccttgca actgagcgat ctcgttgcgc
     3361 tggttcgcca ccctcagggg gcgtttaaaa acgtaccgca aggttgtcgg cgagtctggt
     3421 tcattaaccg tttttctcaa tgtgagaatg cgattgcaca aagcgagctc cttcaaccgc
     3481 tgcaacaaca cgacgtagag gcaatctggc tgggcgatat acaagaacat cctgcaatcg
     3541 cgcgcagatt tgtgaattag cggcccgggt attccgggtt actgaaagga tatccattga
     3601 ggttggtatg aatattttca cagaggctgc aaaactcgaa gagcaaaatt gtccgtttgc
     3661 gatggcgcaa attgttgata gccgaggctc gactccccgc cattctgcac aaatgttagt
     3721 gcgcgccgat ggttctatcg tcggtacaat tggtggcgga atggttgagc ggaaggtgat
     3781 tgaagagtca cttcaggcat tgcaggaacg taagccgcga ttattccatg gacgtatggc
     3841 tcgtaacggt gcggatgctg tcgggtcaga ttgtggaggt gcaatgtcag tatttatcag
     3901 cgtccatggt atgcgtccac gtctggtgtt gattggcgcg gggcatgtca accgggcgat
     3961 agcccagagt gcggcgctat taggatttga tattgccgtt gccgatattt atcgcgaaag
     4021 cctcaatcct gaactattcc caccatcaac cacgcttctc catgctgagt cgtttggtgc
     4081 ggcagtggaa gaactggata ttcgccctga taattttgtc ctgattgcca cgaataatca
     4141 ggatcgtgaa gccctcgaca aactcattga acagcccatt gcatggttgg ggttgctggc
     4201 aagtcgccgc aaggttcagc ttttcctgcg tcaattgcgt gagaaaggcg tggctgaaga
     4261 acatattgcc cgtttacatg cgcccgttgg ttacaacata ggtgcggaaa cgccgcagga
     4321 gatcgccatc agcgtgctgg cagaaatatt atgggtgaaa aataacgcgc cgggtgggct
     4381 gatgatgaaa ccttctcatc cttccggaca ccagctggtg gtgattcgcg gtgcgggaga
     4441 tatcgccagt ggtgtggcgc tacgtctgta tcatgcgggt tttaaagtga tcatgctgga
     4501 agtggaaaaa ccgacagtga ttcgttgtac cgtggcgttt gcccaggccg tgttcgatgg
     4561 cgaaatgacg gtcgaaggcg tcactgctcg cctggcaacc agctctgcgg aagcgatgaa
     4621 acttaccgaa cgcggattca tccctgtgat ggtagatccc gcctgttcat tgcttgatga
     4681 actgaaaccg ctttgcgtgg tggacgctat tctggcgaaa cagaatttgg gaacgcgggc
     4741 agatatggca ccagtaacaa tcgcgcttgg gccgggcttt actgcaggga aggattgtca
     4801 tgcggtaatt gaaacaaatc gcgggcactg gctcggtcag gtgatttact ctggttgtgc
     4861 gcaggagaat accgatgttc ctggcaatat tatggggcat accacccgac gggtgatccg
     4921 tgctcctgct gcaggcatta tgcgatccaa cgtgaaatta ggcgatctgg tgaaagaggg
     4981 cgatgtgatt gcctggattg gtgagcatga aattaaagca ccgttgacgg ggatggtgcg
     5041 tggcttgttg aacgacggcc tggcagtggt tggtggtttt aaaattggtg atatcgatcc
     5101 tcgtggtgaa accgctgatt tcaccagcgt ttctgataaa gcccgggcga ttggcggcgg
     5161 cgtacttgag gcgttaatga tgttgatgca tcagggcgtg aaagcgacaa aagaagtgct
     5221 ggaagtggct taaatgaaaa tggccggatg gtattaccca cccggcctta aaattactga
     5281 caaatcaccg ttccggtttt gccttcaatc ccttctttcg ctttgctcaa tacggtaatc
     5341 agtgcttcac gacctgcgcg agagcgagca aacgaggcgg cagcttccac tttaggcagc
     5401 atagaacctt tcgcaaaatg accttcttcg ataaagcgtt cagcatcact taatgacagg
     5461 cgatcgagcc actgttcatt ctctttaccg aagttaatgg ccactttttc taccgcagtc
     5521 agaatgatca gcatatcggc atcaatcatt tctgctaaac gggcgctggc ccagtcttta
     5581 tcgataaccg cgctggcacc acgcagatgg ttaccttcac gaataacagg gataccacca
     5641 ccgccaacgg cgatcaccac ctggcctgca tctaccagag ctttaaccgt ttctttttca
     5701 atgatatcaa ccggttttgg cgaggcaaca acacggcgat agccgcgacc agcatcttct
     5761 ttcagagtgt aaccctgttt tgtcagttgc tctgcttctt gctcggtaaa gaacgaaccg
     5821 atcggcttgg tcgggttgag gaatgccgga tcgttagcgt cgacttcgac ctgagtaacc
     5881 agcgttgcta caggtttatt aatgccccga gaaagcagtt cttctcttaa cgcgttttgt
     5941 agatcgtaac caatataacc ctggcttaag gcaacacaga cagacatcgg cagcattggc
     6001 gagtgtgctt cagtttttgc ggcagcttca aaggcttggt taatcatgcc cacctgcggc
     6061 ccgttaccat gagtgacgat aacttcatgt ccttgggcaa ttaaatcaac aattgcctga
     6121 gaagtaattt ttaccgcttt catttgtccg gccaggtcat cgcccagcgc attcccgccc
     6181 agggcgagaa caattttctt actcattttt cctcactcat cattcttgat tgtgtataga
     6241 tccctgacag tggttagagc acgggaggga caaacggttt gcgtcgcagg aaacgacctc
     6301 gcccggcttt gcctgtaaag gtgccgtcac agaaaatcgt ttcaccacgg gataaggttc
     6361 tgacaatcgc gccctgacag gtaaaaccct cccatggcga gtagtcggcg ttgtcgtgga
     6421 gatggcgatg ctgaatttgt tggctctgac gtgggtcgat aatcaccacg tcgccatcgg
     6481 aaccgggcgc taataatcct ttttgcggcc acaggccaaa cagcctggcg ggcattgcgc
     6541 tggttaattc aacaaagcgt tccggtgtta tacgtcccgt catcacgcca ctggaaaaca
     6601 gtaactgcat gcggttttcc acaccgggta agccatttgg gcagcgactg aaatcgcctt
     6661 tagaaatttg caggcgttga gccatcgaga aggtgcagtg atcggtcgcc accacgtcaa
     6721 tcgcaccatc gctgatgcca caccacagtt tgtcctgctc gcgtacgtta cgcagcggtg
     6781 ggctaagaat gaacttcatg ccatcttctg tatcgtaact gcgttcgtcc aacaggagat
     6841 attgtgggca ggtttcaacc cagactggct ggtgattcgc acgggcaaga cgcagataat
     6901 ccagacctaa gccgttagac aggtgcacga tatagagcgg ggcgttaccg gcaatttgtg
     6961 ccaggttaat catgcgggcg atggcttccg cttcgcattc cagagggcga ctcaaggcat
     7021 gatagcgcgg cgcggttaac cctgcggcga taaattccgc ccgcttgctg gcgatagccg
     7081 catcattttc cgggtgcacg gtggtcagcg cgccggattc atgcaagcgg cgtaatgcct
     7141 gcaaaacctc gtcatcgttg agtttgtatt gataggttaa atagagttta aaactgctca
     7201 gtccttcctc gaccatcatc gggatttcgt cgaggattgc gtgattaatg tgctggatca
     7261 caccgtgaaa gctgtaatcg atgaccgctt tatgggcggc ataaccacga taaacctcca
     7321 gttgatggcg taaccgacag ccgtttgggc caaatcccat atggtcaata atggttgttg
     7381 taccgccaca cgcagctgcg cgggtaccgg taaaaaatca tcacaactgc gcgcgatgcc
     7441 gacatcaata ttgaaatgcg tatggacatc cacgccgcca gggaaaacgt aacagccagt
     7501 ggcatcaatt tcttcatacg ggagctgcgg cgaaatattg ttgcccaact ggcgaacaat
     7561 cccgctttca atcagcaaat cctgtttggc ttgtccatct gcgttaacga cagtgccgtt
     7621 tttgatcaat acgcgcatag caaactccaa aaaccctccg gcgaaccgga ggggaataag
     7681 tgaagagtaa ttactctgtt gccagccagc ttaacgggat tgcagcgtac atcgcggcac
     7741 aggtcaccag gtgagatttc caggtttttt cgttaggtgc atgcgcttca ggttctttac
     7801 ccgggccaaa gccgatcacc ggaatgccgt gacggcccat gatagatacg ccgttagttg
     7861 agaaggtcca cttatcaaca accggcgctt tgccaaacag cccttcgtag gcattcacca
     7921 gtgctttaac ggtgaagtga tcttcttcca ctttccaggt cgggaagtag cattcggttg
     7981 ggtaaaccag gccagtccag gacggacggt cgtagttgta catagaaaca accgcgttag
     8041 ctttctgtac tgcaggcagg gcgcggattt cgtccagcgc gccttcccag gtttcgcccc
     8101 aggtcagacg gcggtcaata gagacggcgc agctatctgc tacagcgcaa cggcttgggg
     8161 atgtgaagaa gatttcagaa acggtgaggg tgcctttgcc gaggaattcg tcataaccca
     8221 gacgttggga gagttcttgt aattcgccaa gaatcggacc cattttgaaa atggcgttgt
     8281 caccgcgttc tggcgcagaa ccgtggcagc taacaccctg aacatcaata cgaatttcca
     8341 tacgaccgcg ctgaccacgg tatatctggc agtcggttgg ttcggtactg accacaaatt
     8401 ccgggcgaat gccggattgt tcaataatgt actgccagca cagaccgtcg cagtcttctt
     8461 cctgcacagt accggtaacc agcagggtat attcatcttc cagaccgagg tctttaatga
     8521 ttttaccggc ataaaccata gatgccatgc cgccttcctg gtcggaagta ccgcgaccac
     8581 cgattagctc atcagtttcc atgccttcgt acggatcgaa gtcccagttt ttgatgttgc
     8641 caatgccgac ggtatcgata tgagcgtcca ttgccaccag acgcgggccg tggccgatat
     8701 aaccgagaac gttgcccatc gggtcgattt caactttatc gaagccgact ttttccatct
     8761 cttctttaat acgatgtact acgcgttttt cgtcgcagct ttcactggga atagcaacca
     8821 tgtcgcgcag gaagcgagtc atgtccgcct ggtaatcttt tgctttttca agaatcagtt
     8881 tgaatggaat attcttagcc attatttttc tccagatttt ttactccccg ttttgcaggg
     8941 agtagttcca atagggtgat taaggtgcta cagcgtgttt gccttcccag acaacttcgc
     9001 ggtagtgctt cacgtcggtg tcgccttcag tgctgataac cagcactacg gcatctttgt
     9061 ccagcgccag tttttccatc aggctttgat gttgcgggtg ataatgaacc gctgcgagaa
     9121 cgcccaaacc gacagcgccg gattcaccgg agatgatgcg cgggtcgttg ccgtacggat
     9181 tacccagcac gcgcatacct aatgcggcaa cgctgtcctg gcaggagatg aattgggtgg
     9241 cgcagttacg taggatttcc cagcccagcg ggttaggttc gccacaggcc aggcccgcca
     9301 tgatggtggc catatcaccg ccaacgttga cgatgtcgcc tttgacgccg gagcgataaa
     9361 tacagtcagc tttgtcaggt tcaacaataa tgctgtgcag attttgcggg ctgtagacgt
     9421 cgaccagata acccagcaca ccaccggcca ttgctccgac accggcttgc agcagaacgt
     9481 gcgtcggggt tacgcccatt tcacgcattt gctcgacggc ttcatctgcc agggttgcgt
     9541 agccttgcat gatccaggtt gggattttgg tgtaaccttc ccatgccgtg tcctgtacca
     9601 cttcccagcc gtgctgctgc gcgtgttgca tggtcaggcg aacggtatcg tcatagttca
     9661 tatccgtgac gatgcactcg gcaccgaggt tcagaatggc gtcaacgcgt tcctgagcag
     9721 aacctttcgg catgtaaatc accgcattct gtccgagttg ctgtgctgcc cacgccacac
     9781 cgcgcccgtg gttgccgtcg gtggtggtcg cgaaagtcat tttttcgccg atggcatttt
     9841 tcaggtgctc aaatgacagc gtttcgatat caagatgata tttttcacac aataattgag
     9901 cgatggcgta cgcaccgcca agcattttga aggcgttcag accgaatcgt tttgattcgt
     9961 ctttgacgag aattttcttc acaccaaaaa ggtttgcgag atcgtccagc gcacaaagcg
    10021 gtgttgggcg ataaccagct attttctggt ggaactggcg cgccagtttg gcctggcttt
    10081 gcgaaaagag cggtgatgtt tcgccgttga aaaatttgtt atcggcgata tcaatcttca
    10141 atgagaaaac ggacatatcc tatccttttg gcctctggaa atagagggcc gaccagcaga
    10201 tcggcccgag gggttatttc acgcgttctt gcgcttcttt cagcagttgc tcgagcagtg
    10261 cacctggttt ggcgtattta cgggacagga tcatcgcggc gatgatataa ggcttccagc
    10321 tggcttcttt gtaggtagcg atacggtatt tttcgaatac gccttcagtc acttcacctt
    10381 ctttgcagga tacgccgctg atatcagctg gcaggcagtg catgtacagg gcttcgccat
    10441 cacgggtcag ttccatcatc tcttcagtac aatgccagtc tttgtgttgc gcgttctgtg
    10501 ccagacactg tttttccagt gcttttaagc cttcgtggtc gttcgcacgc agcaattcag
    10561 tacgctcttc catcactttg taaggtgccc atgacttcgg ataaacgatg tctgcgtctt
    10621 tgaaggcttc ttccatgctg gtgacctgac ggaagctacc accggaggct ttagcattgt
    10681 ttttcgccac ttcaaccaca tccgggatca ggtcgtagcc ttccggatgg gccaaggtga
    10741 catccatacc gaagcgagtc atcagaccga tgatgccttg tggtacagag agcggtttgc
    10801 catagcttgg agagtaggcc caggtcatgg cgattttttt acctttcagg ttttccagtg
    10861 aaccaaagtg ttcacgtaac cacgcgaggt cagccattga ctgagtcggg tggtcaatat
    10921 cgcattgcag gttcactaaa gccggacgct gtggcagtac accctgcttg taaccgtcat
    10981 caagtgcagc gccaacttca cgcatatagg cgttgcctgc gcccagatac atatcgtcgc
    11041 gaataccaat agcgtcggcg cagaaggaga tcatattggc ggtttcacgc acggtttcgc
    11101 cgtgagcgat ttgtgatttg ccttcgtcga gatcttgttg tgcaaggccg agcaggttaa
    11161 gcgcggaagc ataagagaag cgggtacggg tggagttgtc gcggaatacg gaaataccta
    11221 atccactatt aaagactttg gttgagatgt tttcagcacg cagtgctttt aatgctgcgg
    11281 caacgtccag tacttgtttc agttcatctg gcgtctgttc ccacgttaac aaaaaatctt
    11341 tctcgtgaag gtgagaggtc agcgaattga tatccttaat cagctcatta acagttttca
    11401 tcatacagtc ccggtagtgg aatgacaggc aaagtggttt atttcactgt gacttgccaa
    11461 agtgattggt gttttcatag ctatacctgg gactaacaat aagtgtgcca gaattatccg
    11521 cgcggctgca aaagactaat ttgacggata atgtgtgggg ttgttcacaa attgatgcca
    11581 gcttctggtt gtttgtaaaa tacgaaaacg tttggctttt tttatcgcgt tatttatttt
    11641 ctgctaagta tcagaaataa gataaagggg caggctatct ctcttcagtt tgataaactc
    11701 accgccgtaa ttctcatatt gatatagaaa tgtgatatct cttccataat tgcattagaa
    11761 aatgataaaa atcttatgtt tttatcaaag ccaatttcgc ctgagttaat atcatcatga
    11821 tctgtgaacc atcaacgtct tcaatgatcg acttttaagt aagtctggaa ggtaaagcta
    11881 tggagcttgc tactacgcag tcagtattga tgcaaattca accgacaatt cagcgttttg
    11941 ccagaatgct tgccagcgtt ttgcagtttg aggttgagat cgttgatgaa aacttgtgtc
    12001 gcgtcgccgg aacgggcgcg tatgggaagt ttcttggtcg ccagttgagc ggcaactcac
    12061 gcctgctccg ccacgtcctg gaaacgaaaa ctgaaaaagt tgtgacacag tctcgcttcg
    12121 atcccctttg cgaaggttgc gatagtaaag aaaattgccg cgaaaaagca tttctgggta
    12181 cgcctgtcat tttacaggat cgttgtgttg gggtgataag tttgattgcc gttacccacg
    12241 agcaacaaga gcatatcagt gataatttac gcgaattttc tgattatgtt cgccatatat
    12301 ccaccatttt tgtttcgaaa cttctggagg atcaggggcc aggagataac atcagtaaaa
    12361 tattcgcgac catgatcgat aatatggatc agggcgtatt agttgttgat gatgaaagtc
    12421 gggttcagtt tgttaatcag actgccttaa aaacacttgg tgttgtacaa aataatatta
    12481 ttgggaaacc tatccgtttc agaccattaa catttgagag taattttact catggacata
    12541 tgcagcatat tgtttcgtgg gacgataaaa gtgaattaat cattggtcaa ttgcataaca
    12601 ttcagggccg acaattattt ttaatggcat ttcaccaatc gcataccagt ttttctgtag
    12661 caaatgcacc tgatgaacca catattgaac aattggttgg cgagtgccgt gttatgcggc
    12721 aattaaaacg actcattagc cgtattgcac ccagcccatc cagcgttatg gtggttggtg
    12781 aaagcggcac gggtaaagaa gtcgtcgccc gagcaatcca taagttgagc ggaagacgga
    12841 ataaaccctt tattgctatc aactgtgccg cgattccgga gcagcttctg gaaagcgaac
    12901 tgttcggtta tgttaaaggc gcatttactg gtgcttctgc caacggtaaa acagggttga
    12961 ttcaggcggc gaatacgggc acgctgtttc tcgatgaaat aggtgatatg ccattaatgt
    13021 tgcaggctaa attactgcgc gctattgagg cgcgtgaaat tctgccgatt ggtgccagta
    13081 gcccaataca agtcgacatt cgcatcattt ctgcaactaa tcagaatttg gcccagttca
    13141 ttgccgaagg taaattccgc gaagatctct tctaccgact taatgttatc ccgataactc
    13201 tgccaccgct gcgtgaacgt caggaagata ttgaactatt ggtgcattac tttttacatc
    13261 tgcatacccg tcgtctggga tcggtttatc ctggcattgc tcccgatgtc gtcgaaatat
    13321 tgcgtaagca tcgttggccc ggaaacctgc gcgagttaag caatttgatg gaatatctgg
    13381 ttaacgtggt tccttcaggt gaagttatcg acagcacgct attgccgcca aatctgctga
    13441 ataatggcac aacggagcaa agtgatgtaa cagaggtcag tgaggcgcac ctgtcactcg
    13501 atgatgcggg cggcacggcg ctggaggaga tggaaaagca aatgatccgc gaggcgcttt
    13561 cacgtcataa cagcaagaag caagttgctg atgaactggg catcggcatt gctacgctct
    13621 atcgcaagat taagaaatat gagttgttaa acacataacc tcagtgaatc cggcctgaat
    13681 tcaggccgga tatcctttta cttcgttttc tcgcaatcca gaactgtatt tacaatcatc
    13741 tgataccccg tgcagcgaca aagatttccc gccagtccgc gacgaatttc cgtaatggtt
    13801 aatggcttct cgcgtggttt cgccagcatt gccgtggtag ccataatcag gccaggcgta
    13861 caaaacccgc actgcactgc gccggatttc gcataagcct gctgaacatg agaaagtttt
    13921 ccgcctttcg cttcaccttc cagcgtgcgg atctcctttc cttcagccca ggcggcaagg
    13981 tataagcaac tgtctattgc tgtgccgtcg accaacaccg tacaggcacc acattcaccc
    14041 acgcagcacc cttgtttgac acttagcagt ccttgttcgc ggagtaattc cgagagcggc
    14101 gtgcctggtg cggcgtgaag ctgaaaaggc atcccgttaa tggtgcattc gatggtaatt
    14161 gtttcgctgt gattcattgc aattttcccc ccgccgcggc gacggcttcg ctaatcactt
    14221 ttttggtcat cgtctggatg agatgcagac gaaactcttt actggcccgc catgaagaac
    14281 gcggggcgac atcttgcagg acagattcgc tgatagcttc cagcgtttgc aggtttaatg
    14341 gcgcattttg tgcagtctgt tcggcatgtt ggcagcgaat cggcgttggc gcggcaacac
    14401 caaatgccag gcgtaattcg ctgaaattgc cgttatccag tcggcaatgt gcggcgcagc
    14461 caatcgttga aatatccatt gcgtcgcgca tggcatattt aaaatgcgcg ctgcccgcgt
    14521 gttctttcgg ctgtggcgga aaatgaaagg cgacgaggat ttcgtcatgc tcaagagaca
    14581 ctttgcccgg cccggtgtga aagccattaa tcgggacgaa acgaacaccg cgcggggagt
    14641 ggatctccag tttcgcgtca taaattagcg ttggcgtggc agaatctgcg ctggtggcac
    14701 cgttgcaaat atttccaccg taggtagcga cgttacggat ctgcggtcca gcaatggacg
    14761 tggccgcagc acataacgcc gggagatgac gttgagttat aggagcttct attagctggg
    14821 taaatgtcgt tgcagagccg atacgtagcg agccatcttc cgccagcgta attccccgca
    14881 gctccgccag attatgaata tcaacaatat ggcgataacg gtcattgtga tggtggagct
    14941 gaatcagtac gtcagtgcca tcggcgagca gtttggcctg cgggttgtca gccagcaggt
    15001 tgatggcatc ggcaagggtt gctgcgcgat ggtaagaagc aaaatcaaac atgatgttat
    15061 cctcaaatca atcctgccag atggaactct tcatataacc gttttggcgt cagcggcagt
    15121 gtattgattg caacaccggt agccatcttc accgcgttac gaatagcagc ggcaacagga
    15181 attattggtg gctcacccag tgacttatgt ccgtatgcgg attgcggctc attgatttcg
    15241 acgaacgcgc tttccagttg tggcagatcc ggcatggtcg gcattttgta atccagcaga
    15301 ttggggttac ggaccacgcc gcttttagca tcgatgatca tctcttcaaa tagcgcccag
    15361 ccaatgccca ttcccattcc gccgtgtacc tgaccttctg ccagcagtgg attaagaata
    15421 tgccctgaat catgaacgtt gaggatgcgg ttgatggtga ctttgcacag cgcaatatcg
    15481 accgtcagat caacaaaggt acagccaaac gccgatgggt tagtggtggt tttgatggag
    15541 ctttcagcag agagctgccc gccgcgttca gggtggtaga aagcgtccat cgccaaatct
    15601 tttaacgaca ttaacggctc ttccggtcgt tcaaccagca cgatatggcc ttttatcagg
    15661 gtcagattca tcgctgactg atgtagcatg actgcggcgt gagcgatgat tttctctttt
    15721 aataatagtg ccgcactgcg cagcgcaggc gcggcaacat agctctggcg tgaggcaaat
    15781 gcgccgggag cgaacggcgt aacgtcggta tcttgagttg aaataacgcg aacgtcgctg
    15841 accggaaccc ccacggtttc tgccaccatt tgcgagaaga cggtgtcggc accctgaccg
    15901 atttccgtcg cgccgctttg cacgttgatg gttccatcct gattcatcag aaggcgcgcg
    15961 cctgctattt ctacgccgac aggccaggtg ttagaggtat agctaaaaca ggcgacgcca
    16021 acgccgcggc gcaaattgcc ttgctggttc tggcattctg cacggcgttt ttcccattca
    16081 aagattttcc ggcctttttc aagacactcc ggcaaccctg cgctgtaaat acgtttgccc
    16141 gtgagcggat tagcatctcc ttcgcgggcg gcgttgcgta aacgaatttc aacaggatca
    16201 atacctaacg ctgtcgcggc gtcatcaagc atagactcaa cggcaaatac gacttgtggc
    16261 gcgccataac cacgcatcgc accagccgag gggaggttgg tatagcaggt ctttgaactg
    16321 taagcgtagg cacaacgagg ataaaggtaa gcgactttat tcctccccgc agaagcgatg
    16381 gagtgcccgt gagatgcata agcgccggtg ttagacagaa catccagact ataacctttc
    16441 aatgttccgt cgcggttcac gcccatttgc ccgtcaatgg taaaagcgtg gcgggtacgg
    16501 gttgcgagga aacactcttc acggctaagg gaaactttca ccggaatgcc gccaagcttg
    16561 ctggtcagga atgctgccat tggctcttcc agtacatcct gtttattacc aaaaccgcca
    16621 ccgacaaacg gtttgatgac tcgtacgcat gaccagggaa tatccagcgc ctgaccaacc
    16681 acgcggcgaa caatgtgcgg gatctgggtg ctggaaacga tggtaattcg cgagtcatcc
    16741 tccatccacg ccagcgatgt tacgctttcc atgtgacaat gttgaataac gggggtctga
    16801 tagtgcccct gtacctggta gtcggcggca tcgattgttt gttggacatt acccgtcgac
    16861 atcgtgcttt gtttcagtaa attgccaccg ttatggattg gtgcagcgtc ttctgccagc
    16921 gccgcttctg gcgtggtgat aacgggtaat tcttgccact caatgctgac caattgcgcc
    16981 gctttttctg ccgtgagttc atcgcgggcc acgacgatgg caacggcgtc accatgatga
    17041 cgaacatggc gagttagcag tgcgcgatcg gcggtatcgc gcttgttttc gtcaagtgtc
    17101 caggcatgcc cagctgtagc gaatggaata tcaggcacat cttcccaggt aaaaatcgcc
    17161 agtacgcccg gcaaacttct ggcttgttca tcattaatac ttacggcata accatgtgcg
    17221 atagggctac gtacatattt cgcataacac atgcccgcca taacataatc gtcagtatat
    17281 cgtgcccgcc cggtgacctt agcaatggca tcgacgcgca tgcatgattc acccgtagcg
    17341 gttgcttccc gcgcttccat agaaatcccc tcgatttgaa catttggttc ttatggcaaa
    17401 gatttagtct aagcaggcaa gatttacgcc agaaacgacg ataagcgtta ggggaggtag
    17461 gaagtgtgat gacgttaaaa aaacacagat atgatacgaa acaggactct gaataggggc
    17521 agtgatacgt atcaatatga gtggcgataa aatcaatgtg ataaattggg attattacgc
    17581 atcaggatgg gaaatagggt aaaaagtcag gaagttcgat acctctacag cgaatatttt
    17641 gctgatgata cttccctttg ccaaatgaaa ttaacctctt tctctattac cacgtttttc
    17701 cagaagcaag agattgggta tcaagaggct ggctgctatg ataaggcgtc ttgtttttta
    17761 agcgaggaaa gattttgagt gcgggacgcc tgaataaaaa atctctgggt atcgtgatgt
    17821 tgttatcggt tggactgctt ttggcgggct gttcgggtag caaatcatcc gatacaggaa
    17881 cgtattccgg ctccgtttac accgtgaaac ggggggatac gctatatcgt atttcgcgca
    17941 ccacgggaac cagcgtaaaa gaactggcgc gactgaacgg catttccccc ccttacacca
    18001 ttgaagttgg tcagaaacta aaactgggtg gggcgaaaag tagcagtagt acacgtaaat
    18061 caaccgccaa atcaacgacc aaaaccgcat cggttacacc gtcatcagcg gtaccgaaat
    18121 catcctggcc gccagtaggg caacgttgtt ggttatggcc aacgacaggg aaagttatca
    18181 tgccgtattc gacagcagat ggcggcaata aagggattga tatctcagct ccacggggta
    18241 cacctattta cgccgcgggt gcaggaaagg tggtgtatgt gggcaaccag ctgcgtggct
    18301 acggtaatct catcatgatt aaacacagtg aagattacat tacggcttac gcccataatg
    18361 acacgatgct ggtaaataat gggcaaagcg tgaaggctgg gcaaaaaatc gccactatgg
    18421 ggagcacgga tgcggcatct gttcgcctgc atttccagat tcgttaccgt gcaacggcaa
    18481 ttgatccgct acgttacttg ccgccgcagg gcagcaagcc aaaatgctga tggcgaatta
    18541 atcagcagtc agcagcgcgg cacttgctaa ggagagcgta aggtttataa tgccttacgc
    18601 atctcgaagc gggcgtagtt caatggtaga acgagagctt cccaagctct atacgagggt
    18661 tcgattccct tcgccgcttt tgctgtatct gattttctat attatcttaa ttcaatcgct
    18721 tcagactttt cgcaatcgcc gtaatatttc cccattttgt tgtaaataaa aagcgtttac
    18781 gcaatgcaat ccttttgtaa caacccaggt aaaacacctt attttcaagt tgttaaataa
    18841 gtggttattt acgcttttaa caaacgattg atggaatatt ttatgaaagg gaaatctgca
    18901 ttaactcttc tgctcgctgg tatattttcg tgcggcacat gccaggcgac aggggcagaa
    18961 gtgacgagtg aatctgtatt taacattctt aattccacgg gggcagcaac agataaaagt
    19021 tatttatcgt taaatccaga taagtaccct aattatcgtc ttctgattca ctcagccaaa
    19081 ttaaaaaatg aaataaagag ccactatacc aaagacgaaa ttcaagggtt gttaacatta
    19141 acagaaaaca cacgaaagtt aacgttgacg gagaaaccct ggggaacatt tattcttgct
    19201 tctactttcg aagatgacaa aacagcggca gaaacccact atgatgctgt ctggctgagg
    19261 gatagtttat ggggctatat ggcattagta tcagatcagg gaaatagtgt agccgcaaaa
    19321 aaggttctgc tcaccctgtg ggattatatg tctacgctcg accagattaa gcggatgcag
    19381 gacgtcatta gcaaccccaa acgactggat ggagttcccg ggcaaatgaa tgctgtgcat
    19441 atccgatttg acagcaactc ccccgtgatg gcagatgtgc aggaagaagg caagccgcag
    19501 ctgtggaacc ataaacaaaa tgatgcgtta ggtctttatc ttgatctcct tatacaagca
    19561 atcgacactg gtacaattaa cgctgaagat tggcaaaaag gtgaccgatt aaaatcggtt
    19621 gcgctgctga ttgcttatct ggataaagcc aatttctatg ttatggaaga ttccggagca
    19681 tgggaagaag atgcccgatt aaacacatct tccgttgcac tggtgacctc tggactggaa
    19741 cgtttgtcga acttactctc gaagaaagac tctgtttttg tctctgattt actacgcgaa
    19801 gctaaagcca atgaactgga tgaacctctt tcaacaacga ggttgaacca cctcatagat
    19861 aaaggttatg aaagaataac gttacagctt gatttagggg gagaatcccc aggttatctg
    19921 gaaaaagata aacattacag agaagccgat gcggctttac ttaatgttat ttatcctgca
    19981 aatcttgcaa agataaacac cagaagaaaa gagcaggttc taaaaatagt taaaaaacta
    20041 gcaggtccgt atggtataaa aagatacgag aaggataatt atcagtcggc aaatttttgg
    20101 ttcaatgata ttaaaaccga taccgatcaa aattcacatg cgaaaag
//
LOCUS       DAJVQG010000073        20077 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJVQG010000073 DAJVQG010000000
VERSION     DAJVQG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20077
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.073"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            59..568
                     /gene="radC"
                     /locus_tag="OQM83_002307"
                     /pseudo
     CDS             59..568
                     /gene="radC"
                     /locus_tag="OQM83_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            785..1021
                     /gene="rpmB"
                     /locus_tag="OQM83_002308"
     CDS             785..1021
                     /gene="rpmB"
                     /locus_tag="OQM83_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCS1520561.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            1042..1209
                     /gene="rpmG"
                     /locus_tag="OQM83_002309"
     CDS             1042..1209
                     /gene="rpmG"
                     /locus_tag="OQM83_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCS1520562.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1307..2116
                     /gene="mutM"
                     /locus_tag="OQM83_002310"
     CDS             1307..2116
                     /gene="mutM"
                     /locus_tag="OQM83_002310"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCS1520563.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2155..2634)
                     /gene="coaD"
                     /locus_tag="OQM83_002311"
     CDS             complement(2155..2634)
                     /gene="coaD"
                     /locus_tag="OQM83_002311"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCS1520564.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2642..3919)
                     /gene="waaA"
                     /locus_tag="OQM83_002312"
     CDS             complement(2642..3919)
                     /gene="waaA"
                     /locus_tag="OQM83_002312"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCS1520565.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4332..5390
                     /gene="rfaQ"
                     /locus_tag="OQM83_002313"
     CDS             4332..5390
                     /gene="rfaQ"
                     /locus_tag="OQM83_002313"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCS1520566.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5387..6511
                     /locus_tag="OQM83_002314"
     CDS             5387..6511
                     /locus_tag="OQM83_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCS1520567.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6504..7301
                     /gene="rfaP"
                     /locus_tag="OQM83_002315"
     CDS             6504..7301
                     /gene="rfaP"
                     /locus_tag="OQM83_002315"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCS1520568.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7317..8333
                     /gene="waaO"
                     /locus_tag="OQM83_002316"
                     /gene_synonym="rfaI"
     CDS             7317..8333
                     /gene="waaO"
                     /locus_tag="OQM83_002316"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCS1520569.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFINAKEASPWKNEPFMRPINSNYARYCAKHNFKQNEPINGIMNYIYYFYL
                     KIIK"
     gene            8350..9345
                     /locus_tag="OQM83_002317"
     CDS             8350..9345
                     /locus_tag="OQM83_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HCS1520570.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNVSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNIKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            9355..10047
                     /gene="rfaY"
                     /locus_tag="OQM83_002318"
     CDS             9355..10047
                     /gene="rfaY"
                     /locus_tag="OQM83_002318"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCS1520571.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIND
                     TKVSLIDTKYCRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            10073..11098
                     /locus_tag="OQM83_002319"
     CDS             10073..11098
                     /locus_tag="OQM83_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HCS1520572.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            11183..12166
                     /locus_tag="OQM83_002320"
     CDS             11183..12166
                     /locus_tag="OQM83_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCS1520573.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFE
                     LLNQLVESNEKELNDCNLSGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            12212..13465
                     /locus_tag="OQM83_002321"
     CDS             12212..13465
                     /locus_tag="OQM83_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCS1520574.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLITPKKTITNNPTLLIFISLCLLGIINIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNELRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALLLIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNNLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYIDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQCLLDK"
     gene            complement(13535..14506)
                     /gene="rfaC"
                     /locus_tag="OQM83_002322"
     CDS             complement(13535..14506)
                     /gene="rfaC"
                     /locus_tag="OQM83_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCS1520575.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENVAMI"
     gene            complement(14510..15556)
                     /gene="rfaF"
                     /locus_tag="OQM83_002323"
     CDS             complement(14510..15556)
                     /gene="rfaF"
                     /locus_tag="OQM83_002323"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCS1520576.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(15566..16498)
                     /gene="rfaD"
                     /locus_tag="OQM83_002324"
     CDS             complement(15566..16498)
                     /gene="rfaD"
                     /locus_tag="OQM83_002324"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCS1520577.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            16712..17908
                     /gene="kbl"
                     /locus_tag="OQM83_002325"
     CDS             16712..17908
                     /gene="kbl"
                     /locus_tag="OQM83_002325"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCS1520578.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            17918..18943
                     /gene="tdh"
                     /locus_tag="OQM83_002326"
     CDS             17918..18943
                     /gene="tdh"
                     /locus_tag="OQM83_002326"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCS1520579.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMAEGFDIGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            19182..>20077
                     /gene="waaH"
                     /locus_tag="OQM83_002327"
     CDS             19182..>20077
                     /gene="waaH"
                     /locus_tag="OQM83_002327"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HCS1520580.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSG"
BASE COUNT         5422 a         4377 c         4738 g         5540 t
ORIGIN      
        1 ctgcatgaca aagtcatcgg gcattatctg aacataaaac actatcaata agttggagtc
       61 attacctggc ttgttaacct ctgaatatga acaatttagt ggcgttcatg gaattggcgt
      121 agcgaaattt gcccagttaa aggggattgc tgaactggcg cggcgttact acaacgtgcg
      181 gatgcgtgaa gaaagccctt tactcagccc ggagatgacg cgggaatttt tacaaagcca
      241 gctcacgggt gaggagcggg agatctttat ggtgatcttt ctcgactcac aacaccgggt
      301 tataacgcat agccgtcttt tttccggcac gctaaaccat gttgaagtcc atcctcggga
      361 aattatccgg gaagcgataa aaataaacgc ctcggcgctg atccttgcac ataatcaccc
      421 ttcgggttgt gctgaaccca gtaaagcgga taaactcatt actgaacgga taataaagag
      481 ttgtcagttc atggatttac gcgtgctcga ccatatcgtg attgggcgtg gagagtatgt
      541 ttcttttgcc gaacgcggct ggatttaacc cgctatgcgc gatccttcgg gatctttgtc
      601 tgttcgggac ttgagcacat cgctgagtca gcgtatacta cgccaccttt gagaatctcg
      661 ggtttggcat ttgggcctgg caatcgagag ttcacataga actgcgatga ccgggctgta
      721 aagcctgacg aggcgccaat accccatacg aagctcgagc taatttgatt tttggagaat
      781 agacatgtcc cgagtctgcc aagttactgg caagcgtccg gtgaccggta acaaccgttc
      841 ccacgcactg aacgcgacta aacgccgttt cctgccgaac ctgcactctc accgtttctg
      901 ggttgagagc gaaaagcgtt ttgtcaccct gcgcgtatct gctaaaggta tgcgtgtaat
      961 cgataaaaaa ggcatcgata cagttctggc tgaactgcgt gcccgtggcg aaaagtacta
     1021 agtacttaga ggaaataaat catggctaaa ggtattcgtg agaaaatcaa gctggtttct
     1081 tctgctggta ctggtcactt ctataccact acgaagaaca aacgtactaa gccggaaaaa
     1141 ctggaactga aaaaattcga tccagttgtt cgccagcacg tgatctacaa agaagcgaaa
     1201 atcaaataat tctcgctttg atgtaacaaa aaacctcgct ccggcggggt tttttgttat
     1261 ctgcttgccc ccatattgat tgcatctgtt cattcctgga gatgctatgc ctgaattacc
     1321 cgaagttgaa accagccgtc gcggcataga accgcatctc gttggtgcaa ccattcttca
     1381 tgcggtggtg cgcaacggac gcttgcgctg gccggtttca gaagagatct accgtttaag
     1441 cgaccaacca gtgcttagcg tgcagcggcg ggctaaatat ctgctgctgg agctgcctga
     1501 gggctggatt atcattcatt tggggatgtc tggcagcctg cgcatccttc cagaagaact
     1561 tccccctgaa aagcatgacc atgtggattt ggtgatgagc aacggcaaag tgctgcgcta
     1621 caccgatccg cgccgctttg gtgcctggct gtggaccaaa gagctggaag ggcacaacgt
     1681 gctggctcat cttggaccgg agccgcttag cgacgatttc aatggtgagt atctgcatca
     1741 gaagtgcgcg aagaaaaaaa cggcgattaa accgtggctg atggataaca agctggtggt
     1801 aggggtaggg aatatctatg ccagcgaatc actgtttgcg gcggggatcc atccggatcg
     1861 gctggcgtca tcactgtcgc tggcagagtg tgaattgtta gctcgggtga ttaaagcggt
     1921 gttgctgcgt tcgattgagc agggtggtac aacgctgaaa gattttctgc aaagtgatgg
     1981 taaaccgggc tatttcgcgc aggaattgca ggtttacgga cgaaaaggtg agccgtgtcg
     2041 ggtgtgcggt acgccgattg tggcgactaa acatgcgcag cgggcaacgt tttattgtcg
     2101 gcagtgccag aagtaattca tacgcgccgg atggcatacc atccggcata aacgctacgc
     2161 taacttcgcc accagcgcct gatggacatt ctccggcagg aaatgggtga catcgccctg
     2221 atggcgcgcc acctctttca ccaacgatga agagataaac gaccactctt tcgacggcat
     2281 cagaaacaca ctttccagtt ccggcattaa gtggcgattc atatgcgcca gctgcatttc
     2341 atattcaaaa tccgccaccg cacgcaggcc acgaatcagc accgtagcgt gttgattacg
     2401 ggcgaagttc gccatcaaat cactaaaccc aaccacttcc acgttcccca gatgcgcggt
     2461 tgcctgctgt gtcagcgcca cacgctcttc cagggtaaac atcggttttt tactggggct
     2521 ggcggcaatc gccagaataa cgtgatcgaa catttgcgtg gcgcgcgtca cgatatcgat
     2581 atgaccattg gtaatgggat cgaaagtacc cggataaatc gcccgttttt gcataacaac
     2641 ctcaatgcgt tttcggtggc aggtaaggtt ccagcagttg aagcagacgc tgtagcgcgc
     2701 cctggttttg atacagtact tcaacggcat gacggccata gaaactacgg taatcggcgt
     2761 cggtgagtaa agaggaaacc tcttttgcaa gcgtagtggc atcggtaacg gtaatcagcc
     2821 cgcttgcctg ctccagccgc gcgcaaatgt ctttgaaatt aaaagtatgc ggccccatca
     2881 ataccggaat agcgtgtgcg gcagcttcca gaggattatg ccctccacgt tcaaccagtg
     2941 aaccgccaac aaaggcgaga tcggcaatgc cgtacagtaa catcaactcg cccatcgtat
     3001 cgccaaccac aacctgcgtg ctggtggagg ggacttcccc tgaagagcgt gtgatatagc
     3061 ttagtccagc ctggcggaca aggttaatcg catccgggaa gcgttctgga tgacggggta
     3121 ccaggatgag caataaattc gggaattgct gtaataatgc ctgatgtgcg gcgatcacca
     3181 cactctcttc accttcgtga gtgctggtgg caatccatac cgggcggtgt ggtgcccact
     3241 ggcggcgcag cgtcacggct ttagcagcca actgcggcgt tacagaaata tcgaatttca
     3301 ggctaccggt aacggttacc tggttatttt ttgcgcccag cgccacaaaa cgtgcaccat
     3361 cttcttcatt ttgcgcagca atcagcgtaa tacgacgcag taagcggcgg acgaatttac
     3421 ccagtttggc ataacctgcg gccgagcggg cagagagtcg cgcgttagcg atcaccagcg
     3481 gaattttacg tttatgtagc gccgcaatca ggttaggcca tagttcggtt tccataatca
     3541 acaccagttt ggggtcgact ttattcagga aacggttgag cgcatcgggc aggtcatacg
     3601 gcagataaac gtgctgaaca tccttcccga aagccgattg tacgcgctcc gaaccggttg
     3661 gcgtcatggt tgtaacggta atcggtaaat caggataacg atggcgcagc gcgcgcacca
     3721 acgggatcgc cgccagagtt tcaccgacgg agacggagtg cagcataatg ccgcctggtt
     3781 ttagcggatg gcggtaaaaa ccgtaacgtt caccccagcg ttttcgatag gccggagcct
     3841 tacgtccgcg cacccagagc cgtatccaga tcagcggctg aataaggtag agaagggcgg
     3901 tgtaaagcaa ttcgagcata gtaaatagct gacttatgga tgtgctgggg attctatgta
     3961 tttagctgtg gctttaccat tacttttccc gtttttgact taaatagcaa tttacccgcc
     4021 aattcttgct gaacatctat actttttttg tatttttatg cgattcattg atttctgctg
     4081 tacgtgcagg aaggaatcgc agagcaacag tgaaagaata tggttgctaa tgattttatt
     4141 tgattgattt ctttattgaa aatcaacaat gtgcttaaaa agcattcggg tcggattgcc
     4201 gcgaaagaag tgatacacta gcgcgtcaat atcagtgcgg tatcagtatt caggtagctg
     4261 ttgagcctgg ggcggtagcg tgctttttct gcttcacctt aatcggataa tctcaacaaa
     4321 aagagttact tgtggataag ccatttcgaa gaattttgct cattaagatg cgttttcatg
     4381 gggatatgtt attaactact cccgtcatta gttcgctgaa aaaaaattac cctgacgcaa
     4441 aaatcgatgt gctgctttat caggacacca ccccgatcct gtctgaaaat ccagagatta
     4501 acgcgctcta cggcataaaa aataaaaaag caaaagcctc agaaaaaatt gccaactttt
     4561 ttcatctcat caaggtatta cgtgccaata agtatgacct tatcgtcaat cttaccgatc
     4621 aatggatggt tgctatactg gttcgcttat taaatgcccg tgtgaaaatt tcccaggatt
     4681 atcatcatcg gcagtctgct ttttggcgta aaagtttcac ccatttagtg ccgttgcagg
     4741 gtggaaatgt ggtggaaagt aacttatccg tgctgacacc attgggactt gattcgttgg
     4801 tgaagcagac aaccatgagt tacccgcctg caagctggaa acgtatgcgt cgcgaacttg
     4861 atcacgctgg tgttggacaa aattatgtgg ttatccaacc tacggcgcgg caaatcttca
     4921 aatgctggga caacgccaag ttttccgctg tgattgatgc cttacatgct cgtggttatg
     4981 aagtcgttct gacgtccggc ccagataaag acgatctggc ctgcgtcaat gaaattgcgc
     5041 agggatgcca gacgccacca gtaacggcgc tggctggaaa ggtgaccttc ccggaacttg
     5101 gtgcgttaat cgatcatgcg cagctgttta ttggcgttga ttccgcaccg gcgcatattg
     5161 ccgctgcagt taatacgccg ctgatatcgc tgtttggtgc gacagaccat attttctggc
     5221 gtccttggtc aaataacatg attcaattct gggcgggaga ttaccgggaa atgccaacgc
     5281 gcgatcagcg tgaccgaaat gagatgtatc tttcggttat tccggcggca gatgtcattg
     5341 ctgctgtcga taaattactg ccctcctcca cgacaggtac gtcgttatga tcgttgcttt
     5401 ttgtttatat aaatattttc cctttggcgg tttgcagcgc gattttatgc gtattgctca
     5461 gacagtcgcc gcccgaggtc atcatgttcg ggtttatacc cagtcgtggg aaggcgaatg
     5521 ccctgatgta tttgaactga tcaaagtgcc ggttaaatcg cataccaatc acgggcgcaa
     5581 tgcggagtat tttgcctggg tgcaaaaaca tttacgcgaa catcccgtcg ataaagtcgt
     5641 tggattcaac aaaatgccgg ggctggacgt ttattatgcc gctgatgttt gttatgccga
     5701 gaaagtcgcg caggaaaaag gctttttcta tcacctgacg tcacgttatc gccattatgc
     5761 cgcctttgag cgggcaacct tcgaacaggg caagccgaca cagctgctga tgctgacaga
     5821 taagcaaatc gccgatttcc agaaacatta tcagactgaa gcggagcgtt ttcatattct
     5881 gccaccgggg atttatcctg atcgtaaata tagccagcag ccagctaata gccgtgaaat
     5941 cttccgtaag aagaatggaa taaccgaaca acaatattta ttgttgcagg tcggttcaga
     6001 cttcacgcgt aaaggtgtcg atcgttccat tgaagcactt gcttcgttac cggattcgct
     6061 gcgccacaac acattgctat atgttgttgg acaggataag ccgcgaaaat ttgaggcgct
     6121 ggcagaaaaa cgcggcgtgc gcagtaatgt tcacttcttc tcggggcgca acgatgtctc
     6181 ggaattaatg gcggcggcgg atttattact gcatcctgcc taccaggaag cagcgggaat
     6241 tgtgctactg gaagcgatta ctgcaggatt accggtacta acaacagccg tttgtggcta
     6301 tgcgcattat attgtcgacg ccaattgcgg cgaggctatt gctgagccat tccgccagga
     6361 aacattgaat gagattttac gcaaagcgtt aacgcaatct tcattgcgcc aggcctgggc
     6421 ggaaaatgcg cgacattatg ctgatacaca agatttatac agtctgccag agaaagcggc
     6481 ggatatcata acgggtggtc tggatggttg aacttaaaga gccgtttgcc acgttatggc
     6541 gcggtaaaga tccttttgag gaagttaaaa ccttgcaggg tgaggttttt cgtgaactgg
     6601 aaactcgccg cactctgcga tttgaaatgg cgggcaaaag ctattttctc aaatggcatc
     6661 gcggcacgac cctgaaagag ataatcaaaa atttactctc attgcggatg ccagtattag
     6721 gcgcagaccg cgaatggaat gcgattcatc gactgcggga tgtcggcgtt gatactatgt
     6781 atggggtggc atttggcgaa aaaggtatta atccgctgag cagaacctcg tttattatta
     6841 ccgaagatct gaccccaacc atcagtctgg aagattactg tgctgactgg gcgaccaacc
     6901 caccagatgt tcgcgtaaag cgtatgctta ttaagcgtgt cgcgacgatg gtgcgcgata
     6961 tgcatgctgt gggcattaac caccgtgact gttatatctg tcatttcctg ctgcacttgc
     7021 ctttttccgg taaggaagag gagttaaaaa tttcggtaat tgacctgcac cgggcgcagc
     7081 ttcgcacgcg cgttccacgt cgttggcgcg ataaagatct tattgggctt tatttttctt
     7141 cgatgaatat cggcctgact cagcgggata tctggcggtt tatgaaagtg tattttgtcg
     7201 ccccgcttaa agacattctc aagcaggaac aaggactgct gtcgcaagca gaagcaaaag
     7261 ccacaaaaat cagggaaaga acgattcgaa aatcgttgta atttccggag agaaatatga
     7321 gtgcccacta ttttaatcca caagagatga tcaataagac aatcatcttc gatgaaaggc
     7381 cagcggcgtc agtggcatca tcattccatg ttgcttatgg cattgataaa aactttcttt
     7441 ttggttgtgg tgtttcaatc acgtcagttt tgttacataa caacgacgtg agttttgttt
     7501 tccacgtttt tattgatgat atccctgaag ccgatatcca gcgtttagcc caattggcga
     7561 aaagctatcg tacctgtatc cagatccatc tggtaaattg tgaacggctt aaggcattac
     7621 cgacgaccaa aaattggtct attgccatgt atttccgttt tgtaattgca gattacttta
     7681 ttgatcaaca agataagatc ctttacctgg atgctgatat cgcctgtcag ggaaacttaa
     7741 agccgctgat aacaatggat cttgccaata acgttgctgc tgttgttact gaacgcgatg
     7801 ctaactggtg gtcgttacgg ggtcaaagtc tgcagtgtaa tgaacttgaa aagggttact
     7861 ttaattcagg tgtcctgtta attaatacac tagcgtgggc gcaggagtcc gtttctgcta
     7921 aagcgatgtc gatgcttgct gataaagccg tcgtttcccg tttaacctat atggatcaag
     7981 atatacttaa tcttatcctg tcagggaaag ttaaattcat tgatgctaaa tacaatactc
     8041 aatttagttt aaattatgaa ttaaaaaaat catttgtttg tccaattaat gatgaaaccg
     8101 tattaattca ttatgtcggc ccgacaaaac cctggcatta ctgggccggt tatccaagtg
     8161 cgcaaccttt tatcaacgcc aaagaagcat cgccctggaa aaatgaaccg tttatgcggc
     8221 caattaattc aaactatgct cgttattgcg ccaagcataa ttttaaacaa aatgaaccaa
     8281 ttaacgggat aatgaattat atttattatt tttatttaaa gataataaaa tgatattgag
     8341 aaaagttaaa tgaatgaatt tataaaagaa cggttttcgt atttagcaga taataaaaaa
     8401 gaaaacgccc cagaactaaa tgtttcctac ggtatcgata agaatttttt gtatggtgct
     8461 ggcgtttcaa tttcttccgt tttgattaat aattcagata ttaattttgt ctttcatgtt
     8521 ttcactgatt atgtggatga tgattattta aagtcattta atgaaacagc aaaacaattt
     8581 aatacctcaa ttattgtata tttaattgac ccaaaatact ttgctgatct gccgacgtca
     8641 cagttttggt cgtacgcgac atacttcagg gtattgtcct ttgaatatct gagtgaaagt
     8701 atttccacac tgctgtatct ggatgccgat gtcgtttgta aaggaagtct gaagcctctc
     8761 acagaaatta tatttaaaga tgagtttgct gcggtcattc ctgacaatgt tagtactcag
     8821 gcggcatgtg caaaacgcct caacattccc gagatgaatg gacgttattt caatgcaggc
     8881 gttatctatg tcaatattaa aaaatggcat gaagcaaatt tgacaccgta tttactcacg
     8941 cttttacgag gggaaactaa atatggctct cttaaatatt tagatcagga tgcgttgaat
     9001 atcgcattta atatgaataa tatctacctc gcgaaggatt ttgatactat ttataccctg
     9061 aaaaacgaac ttcatgatcg tagtcatcga aagtatcagc aaaccattac cgataaaaca
     9121 gtattgattc actatacagg gataactaaa ccatggcata gctgggctgg ttatccgtct
     9181 gcatcatact ttaatatcgc gcgtgaacaa tctccctgga agaaatatcc tcttaaagag
     9241 gcgcggactg ttgcagaaat gcagaaacaa tataagcatc tgtttgccca tggtgagtat
     9301 attaaaggca taacttcatt aattaagtac aagcttaaga aataaattac tcttatgatt
     9361 acaagtatac gctatcgcgg cttctcattt tattacaaag ataatgataa taaatataaa
     9421 gaaatctttg atgaaatact ggcctataat tttaaaacag taaaagtatt acgtaatatt
     9481 aatgatacca aagtatcatt gattgatacg aaatattgtc gctatgtttt taaagtcttt
     9541 gccccgaaaa cgaaaaggaa tgaacgtttt ttaaaatctt ttgtaaaagg tgactactat
     9601 cagaatttga tcgttgaaac cgatcgtgtc agaagtgcag gtcttacttt tcctaatgat
     9661 ttttattttc tggctgagcg taaaattttc aactatgcca gtgtttttat catgcttatc
     9721 gagtatgtcg agggcgtaga gctcaatgat atgccgatca ttccagaaga tgttaaagca
     9781 gaaattaagg cttcaatgga aaaattgcat gccttaaata tgctatcggg cgacccacac
     9841 cggggtaact ttatcgttag taaagatggt gtcaggataa ttgacctatc cggtaaaagc
     9901 tgtacggcag agcgtaaagc tcgtgatcgt ctggccatgg aacgtcacct gggtattgcc
     9961 aacgaaataa aagattatgg ctactattca gtaatctaca gaaccaaact acgcaaattt
    10021 attaaaaaat taaaaggcaa agcgtaaacc acacagtcaa aacggaacca acatggattt
    10081 attagctgag agtattactg aagtcgctgt ctctggggaa attgctaaca ccgatcgtgt
    10141 gttaaatatc gcttacggta ttgaccgtaa ctttttattt ggtgcggcag tatctatgca
    10201 atcagttgtt atgcataatc cggaccttgc ggttaagttt catctcttta ccgactacat
    10261 tgatgaagat tatctacaac gtgttaatgc ttttaccagc aaaaatgcta acgttgaagt
    10321 aagaatttat aaagtctcca gtgcctttat tgatatcttc cccagcctga aacagtggtc
    10381 ttatgcaaca ttcttccgtt tagttgcgtt ccagtatctg agtgaaacta ttgaaaatct
    10441 gttatatatc gatgctgatg tcatctgtaa aggctcatta gctggattgc ttgatattaa
    10501 ttttgatggg gataagtttg cagctgttat taaagatgtg ccttttatgc aggaaaaacc
    10561 agcgaagcgt ctggctatag agggacttcc agggaattat ttcaacgccg gtgtagtata
    10621 tctgcagctt gaagcatggg cgaaaaatga ttttatgaat aaagccattg ctatgctggc
    10681 aagtgacccg cagcacacga agtataaatg ccttgatcag gatattttaa atattctgtt
    10741 ctttggtcat tgtattttta ttagcggcga ttatgattgc ttttatggca tcgattatga
    10801 gttaaaaaat aaaagcgatg aagattataa aaagaccatt accgatgata ctaagctgat
    10861 tcattatgtt ggcgtaacga agccctggaa cgactggacg aattatccct gccagaagta
    10921 ttttaatgag gcttatcagg cttcttgctg gaatgatgtg gcgtttattc cagccacgaa
    10981 tgaaaagcag tatcaagtga aatatcaaca tgcaaagaaa aatggtgata cgtttaacgc
    11041 ttttatttac tttattaaat ttaaattaaa taagtataaa agaaaactat ttggataata
    11101 ataaagatca tggatgtctg agttacaata ataattaagg atgagtacta tcatatttta
    11161 ttagttcagg caagagaaca cgatgagcaa tgattaccct ttagtatcca taataatacc
    11221 gacgtataat tcatctgatt acattactga aactctaacg aaattagaaa aacaaactta
    11281 cccaaatttt gaaattgtta ttgttaatga tggttctaaa gataacacat caaacgtttt
    11341 gagagagtat gggttaaccc actctcgatt aattattatc aataaagaaa atggcggtgt
    11401 ttcgtctgcc aggaatacag gtatccgcaa ggcgcaagga cagtttatat gttttatgga
    11461 tgatgatgat gagatagatc ctaactatct gctgaagatg tataccagac aacatgagac
    11521 gggaggggat gcgatttatt gtgggcttta tggccattat ataaaagatg gtgttactta
    11581 ctcacctata aatacagagt ttaatgaagg atctttactt ttcgactttt tttataaaaa
    11641 ggttagattc catatagggt gcttgtttat aagaaaacaa cttctggaag agaataatct
    11701 tttttttgat gaagatttac gactaggaga agatctggat tttatctatc gactgctaat
    11761 tacatgcgat atgtatgcgg ttccatatta tatgtataag cataactata gagaaaattc
    11821 cttaatgaac tcatgtagaa ccatcactca ttatcgacat gagtcatttg cgcacgaaag
    11881 aatctactct tctgtgatgc agttatacaa aggtaacagg aaagaagaaa ttcatacatt
    11941 attgagtcaa aatagagctt atcataaaac tcgttattta tggaatgttc tacttaatgg
    12001 tgattttgaa ctattaaatc aattagttga aagcaatgaa aaagaattga atgattgtaa
    12061 tctttctggc aagagagata aaagacgagc aaaaatatta gcatcaaaaa attatattct
    12121 ctggaggatg gtaagactgg taaataggaa aaagaataaa cgttagatct tgcattaaat
    12181 aatccgtcct cgtagcatag gttgaaacta tatgtcattt tgttggaatg aaattaattc
    12241 tggtataaag tctttaattc tcatattatg tattttttct ttgatgactt tgtctttatg
    12301 ggatgatgtt gcaacaaagt ttcttcatgc agctggaatt atatctgcat tgtattttct
    12361 aataacacca aaaaaaacaa taactaacaa tcctactttg ttaattttca tctcattatg
    12421 tcttttggga atcataaata tcatctggta ttcacattat aaagtttcag gctctgttta
    12481 taccaatgca tatcgtggcc caatggaaac tgggaaaatt gccttgtgta gtgcttttat
    12541 tttcttggtt ctttttgcta aaaatgagtt gagaacaaaa ataaaatttg ggaaactaat
    12601 tctgttcgca tccctggcaa cgcagttact tttttttgcg catgccatgt ggcaacattt
    12661 ctatttaaac gttgaccgtg ttgcattatc agcttcccac gctacaacag caggctacat
    12721 catccttttc ccttctttac tggcatcaat tctcatttta aaatccgact ttagacataa
    12781 aacaacatta tatacaatta acttcatgct tagcttatgt gctgtcatag taactgaaac
    12841 gcgtgcagcc atattagtgt ttccattctt tgcgttacta ttaatcgtaa tggatagtta
    12901 tattaataag cgaattaatt ataagttata ttgttttatt gcgattgcat tattagcagg
    12961 tgtattttct tttaaagata cattgcttat gagaatgaat aacttaaata acgatttagt
    13021 taattattcg catgataaca ccagaacttc agtcggtgcc cgtctggcaa tgtatgaagt
    13081 tggcttaaaa acatattctc caataggaca atcactggaa aaacgagcgg aaaaaataca
    13141 tgaactagaa gaaaaagagc ctagattgag tggcgcttta ccctatatag attctcattt
    13201 gcataacgat ctcatagata cgttatcaac gcgtggtatt cctggagttg tattaacaat
    13261 tttagccttt tcagcaatac tcatatatgc cttaagaact gctaaagaac cttatatttt
    13321 aatcttgctt ttttcactac tggtagtagg cctaagtgat gtaatactct tttctaaacc
    13381 ggttccgact gctgtgtttg tcaccataat attgctttgt gcttatttta aagcacaatc
    13441 agaccaatgt ttattagata aataatgaat atgtgaaata aaatcagcaa ggaggctgat
    13501 tttatttcaa aattatctat taaggggttg taatttaaat catagcaacg ttttcttcaa
    13561 tgaatcgctt aacagcattt gctgtcaatt gagacaactc attccccgga gccctacaaa
    13621 ccatctgatt cttcccatat ccaccaatta atcccggatc ggttggtcca taaaccgtga
    13681 tattgggtct atccagtgcc gccgttaaat ggcttaaccc cgtatccacc gacactacaa
    13741 atttagcccc ggccagcacg cgggcaacgc cttccagact catcttcggc aatacttcaa
    13801 cataagcaaa tccttccgcc agtcgtttcg cccgctcttc ctcatgcggc gcgccccacg
    13861 gaagtttaat ccgtattcct gaatcagcca gtaaaccaat caattctcgc cagtgttctt
    13921 ccggccagtg tttatcatca cgggtcgtcg catgaagaaa tacggcatat tcgccagcat
    13981 ctgtaggcag gttcgtcagg aaatgctgtg caatagcata atcgccctgg gtttgcggtt
    14041 tgctatagcc cagacttttg gcaaacagtt cgcgggtgcg ttctacggcg tgctgctgtt
    14101 ttgcaatatg atgcttacga ttgtaaaaca gactggctaa aggttcgcga gcggtttgcc
    14161 agtccatgcc atgctttacg ccatgcgcca gacgcgtcac cagcgccgcg ctttttacca
    14221 gcccctgagc gtcgataact gcgtcatagt tctctgcttg tagcgcttca cgaaacgctt
    14281 tgcgttccgc ttttatgggg gccgagaacc aggctttacg ccagcgacgt attgccacag
    14341 gaataactcg ctcaacggcg gcgtgccagg aaggaatctg tgcgaaccct tcttccacca
    14401 cccagtcaaa cttaatccct gggattgcct gctgggcatc agtgagtgcg ggcaacgtat
    14461 ggagaacatc gcccatcgac gatgttttaa cgatcaaaac ccgcatccgt caggcttcct
    14521 cttgtaacaa tagcgcgttg agttcttcca gtacgcgctg gggagtaatg tcgattaagc
    14581 tctggtgata accctccgca gcgtcacctt tacgcacttt gtgatagccg gtaatcaggc
    14641 ggatcacgcg cgctttatgg gatagcggcg gtgtgaagtc cgggctactc ggaccataca
    14701 gggcaaccag cggacgattg agcgccgccg caacgtgcat caggccagaa tcgttagtga
    14761 caatggcttt acaggctgca atcaggataa ccgcttgatc aagctgtgtt tcccccgcca
    14821 ggttccgaca ccatgcctgc tgctcggtat tcaaagcggc aagaatctca ttgcccgctt
    14881 catgatcttt cgccgagcca aacagaacca cctgataacc ttcatcaatc agctgctttg
    14941 ccagttccgc atagtggtag tgtggccagc gttttgccgg accaaactcc gcacccgggc
    15001 aaaaaccaat catcggacgt tctgatgaaa gcgaaaattg attacaggta tatgattttt
    15061 caccttcgct cacctgcaat tgcggccata acaacggttt tggtagatct tgtgcggtac
    15121 gcatcacgcc tttgtcatag gccagcgcga catagcgttc caccattagc ggccaggctt
    15181 ctttatcgag cacgcgtaaa tcgttgagta aaccgtagcg catctcgccg cgccagccgg
    15241 tgcgatgagg aatacccgcg aagaaaggca ctaatgcaga tttgaaggag ttgggtaaga
    15301 cgtaggcgcg gtcgtagcgc ttttcacgca ggctatgacc cagtttgcgg cgttcgccga
    15361 tttccagcgc tccgtgaccg agaggcatag gaatagcttc gttaacttcc ggcatccgcg
    15421 ataataatgg acggcaccat gccggtgcca tcacatcgat tatcgcctgg ggatagcgcg
    15481 cctggagcgt gcgatagaga ctttgcgaca tcatcatgtc gccaacccaa gacgggccga
    15541 tcaccagtat tttcatgcag agctcttatg cgtcgcgatt cagccaggcc atgtattccg
    15601 ttacaccttc agcaacggtt ttgaacggtt tgtcgtaacc cgccgcgcgc agatttgtca
    15661 gatctgcctg agtgaacgcc tggtagcggc ctttcagctt atccgggaac ggaatgtatt
    15721 cgatctggcc tttcttgtga taagccagcg tagcatcagc tacagcctgg aaggattccg
    15781 cacgaccagt accgaggttg aagatgccgg aaacgccatt ttccaggaac cacagattca
    15841 catcagccac gtcgcccaca tagacgaagt cgcgtttgaa gttctcgcta ccttcaaaca
    15901 gcttcggtga ttcaccgttg ttaagctggg tgttgagatg gaaagcgacg ctcgccatgc
    15961 tgcctttatg gccttcacgc ggtccataaa cgttgaaata gcggaagcca acaatctgcg
    16021 agttagcttc aggcaggatt tggcgaacat attcatcaaa caggaatttt gagtaaccgt
    16081 agacgttcaa cggtttttcg tactcgcggg attcaataaa gtcggaggtg cgtccgccgt
    16141 aggtggctgc ggaagaggca tacaggaacg ggatttcgcg ctccagacag tagtgcagca
    16201 gctctttgga gtattgatag ttgttatcca tcatatactt gccatcccac tcggtggtgg
    16261 aagagcacgc gccttcgtgg aaaatcgctt cgacatcgcc gaactcttcg ccagccataa
    16321 tctggatcag gaagtcttcc ttatccatat agtccgcgat atccagatcc accaggttca
    16381 caaacttggt gccgtctttc aggttgtcca ccaccagaat atcggtgatg cctttatcat
    16441 tcagggcctt aacgatgttg ctgccgataa agcccgcgcc gccggtaacg atgatcataa
    16501 ctgtaacctt cgaattatgg agtcagagac aatctcagac acgaatactt ctatcatatc
    16561 actacacggc gttggcttca gccattcgcc aaaagtgaga ttgatggacg ttatttatcc
    16621 tgaattttgc agaagtgtta acgcgtcatc tcgtcgcgac ctataagttt gggtaatatg
    16681 tgctggaatt tgccctgtct ggagaatcgc aatgcgtgga gaattttatc agcagttaac
    16741 caacgatctg gaaaccgcac gggcggaagg gttgtttaaa gaagagcgca ttattacgtc
    16801 tgcgcagcaa gcagatatca ctgtggctga tggaagccac gtcattaact tttgtgccaa
    16861 taactatctc gggctggcga attatccgga tctgattgcg gcggcaaagg cgggaatgga
    16921 ttctcacggt ttcggcatgg cttcggtgcg ttttatttgc ggcactcagg acagccataa
    16981 agagcttgaa caaaaactgg cggcattcct ggggatggaa gatgcgattc tctactcttc
    17041 ctgctttgat gctaacggtg gcctgtttga aacgctgctg ggcgcggaag acgccattat
    17101 ctccgacgca ctgaaccacg cgtctattat tgatggtgtg cgtctgtgta aagctaaacg
    17161 ctatcgctat gccaacaacg atatgcagga gctggaagca cgtctgaaag aagcgcgtga
    17221 agccggtgcg cgtcatgtgc tgattgccac cgatggtgtg ttctcgatgg acggcgtgat
    17281 tgccaacctg aagggcgttt gcgatctcgc agataaatat gatgccctgg tgatggtaga
    17341 cgactcccac gcggtcggtt ttgtcggtga aaatggtcgt ggttcccatg aatactgcga
    17401 tgtgatgggc cgggtcgata ttatcaccgg cacgctcggt aaagcgctgg gcggggcttc
    17461 tggtggttat accgcggcgc gcaaagaagt ggttgagtgg ctgcgccagc gttctcgtcc
    17521 gtacctgttt tccaactcgc tggcaccggc cattgttgcc gcatccatca aagtactgga
    17581 gatggtcgaa gcgggtagcg agctgcgtga ccgtctgtgg gcgaacgcgc gtcagttccg
    17641 tgagcaaatg tcggcggcgg gctttaccct ggcgggagcc gatcacgcca ttattccggt
    17701 catgcttggt gatgcggtag tggcgcagaa atttgcccgt gagctgcaaa aagagggcat
    17761 ttacgtcacc ggtttcttct atccggtcgt tccgaaaggt caggcgcgta ttcgtaccca
    17821 gatgtctgcg gcgcataccc ctgagcaaat tacgcgtgca gtagaagcat tcacgcgtat
    17881 tggtaaacaa ctgggcgtta ttgcctgagg atgtgagatg aaagcgttat ccaaactgaa
    17941 agcggaagag ggcatctgga tgaccgacgt tcctgtaccg gaactcgggc ataacgatct
    18001 gctgattaaa atccgtaaaa cagccatctg cgggactgac gttcacatct ataactggga
    18061 tgagtggtcg caaaaaacca tcccggtgcc gatggtcgtg ggccatgaat atgtcggtga
    18121 agtggtaggt attggtcagg aagtgaaggg cttcaagatt ggcgatcgcg tttctggcga
    18181 aggccatatc acctgcggtc actgtcgcaa ctgtcgtggc ggtcgtaccc atctgtgccg
    18241 caacacgatc ggcgtcggcg ttaaccgccc gggctgcttc gccgaatatc tggtgattcc
    18301 ggcgttcaac gccttcaaaa ttcccgacaa tatctccgac gacctggctt ccatttttga
    18361 tcccttcggt aacgccgtgc ataccgcgct gtcgttcgat ctggttggcg aggatgtgct
    18421 ggtctctggt gcaggcccga ttggtattat ggcagcggcg gtggcgaaac acgttggtgc
    18481 acgcaatgtg gtgatcactg atgttaacga atatcgtctc gaactggcgc gcaaaatggg
    18541 tatcacccgt gcggtcaacg tcgctaaaga aaaccttaat gatgtgatgg cagaactggg
    18601 catggccgaa ggctttgata tcggtctgga aatgtccggt gcgccgccag cgtttcgtac
    18661 catgctcgac accatgaacc acggcggtcg cattgcgatg ctgggtattc caccgtccga
    18721 tatgtctatc gactggacca aagtaatctt taaaggcttg ttcattaaag gtatttacgg
    18781 tcgtgagatg tttgaaacct ggtacaagat ggcggcgctg attcagtcag gtctggatct
    18841 ctcgccgatc attacccatc gtttctctat cgatgatttc cagaagggct ttgacgctat
    18901 gcgttcgggc cagtccggga aagtaattct gagctgggat taacacgaac aagggctggt
    18961 attccagccc ttttgcctga ggataatccg ttaaatatgt aaaatcctgt cagtgtaata
    19021 aagatatcgt aattgcgctg atctcttata tagctgctct cattatctct ctaccctgaa
    19081 gtgactctct cacctgtaaa aataatatct cacaggctta atagtttctt aatacaaagc
    19141 ctgtaaaacg tcaggataac ttcagaggtc gtcggtaatt tatgatgaac agcaccaata
    19201 aacttagtgt tattattccg ttatataatg cgggcgatga tttccgcact tgtatggaat
    19261 ctttaattac gcaaacctgg actgctctgg aaatcattat tattaacgat ggttcaacgg
    19321 ataattctgt tgaaatagca aagcattacg cagaaaacta tccgcacgtt cgtttgttgc
    19381 atcaggcgaa tgctggcgca tcggtggcgc gtaatcgtgg gattgaagtg gcaacgggca
    19441 aatatgtcgc ttttgtcgat gctgacgatg aagtctatcc caccatgtac gaaacgctga
    19501 tgaccatggc gttagaggac gacctcgacg tggcgcagtg caacgctgac tggtgttttc
    19561 gtgaaacggg agaaacctgg aaatccatcc ccagcgatcg ccttcgctca accggcgtat
    19621 taaccggccc ggactggctg cggatggggc tttcttcgcg ccgttggact cacgttgtct
    19681 ggatgggggt ttatcgccgc gatgttattg ttaaaaataa cattaaattt attgccggat
    19741 tacatcatca ggatattgtc tggacaacag aatttatgtt taatgcgctg cgtgcgcgat
    19801 ataccgagca atcgttatat aaatattatc tgcataatac gtcagtgagt cggttgcata
    19861 gacaagggaa caaaaacctt aattatcaac gtcactatat taagattact cgtctgctag
    19921 agaaattaaa tcgaaattat gccgacaaaa ttacgattta tccggaattt catcagcaaa
    19981 taacttacga agcattgcgt gtttgccatg cggtgcgcaa agagccggat attattaccc
    20041 gccaacggat gattgccgag atatttactt ccggtat
//
LOCUS       DAJVQG010000074        19954 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJVQG010000074 DAJVQG010000000
VERSION     DAJVQG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19954
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.074"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..753
                     /gene="ypdA"
                     /locus_tag="OQM83_002328"
     CDS             <1..753
                     /gene="ypdA"
                     /locus_tag="OQM83_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCS1520581.1"
                     /translation="KIYYCHAHQITSSLQEMAVGLSQIISTQLEVSRAEQLREMANKA
                     ELRALQSKINPHFLFNALNAISSSIRLNPDTARQLIFNLSRYLRYNIELKDDEQIDIK
                     KELYQIKDYIAIEQARFGDKLTVIYDIDEEVNCCIPSLLIQPLVENAIVHGIQPCKGK
                     GVVTISVAECGNRVRIAVRDTGHGIDPKVIERVEANEMPGNKIGLLNVHHRVKLLYGE
                     GLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            768..1502
                     /gene="ypdB"
                     /locus_tag="OQM83_002329"
     CDS             768..1502
                     /gene="ypdB"
                     /locus_tag="OQM83_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCS1520582.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            1515..2372
                     /locus_tag="OQM83_002330"
     CDS             1515..2372
                     /locus_tag="OQM83_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1520583.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(2375..4870)
                     /gene="ptsP"
                     /locus_tag="OQM83_002331"
                     /pseudo
     CDS             complement(2375..4870)
                     /gene="ptsP"
                     /locus_tag="OQM83_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(4895..5932)
                     /gene="ypdE"
                     /locus_tag="OQM83_002332"
     CDS             complement(4895..5932)
                     /gene="ypdE"
                     /locus_tag="OQM83_002332"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCS1520584.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTAFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(5932..7017)
                     /gene="ypdF"
                     /locus_tag="OQM83_002333"
                     /pseudo
     CDS             complement(5932..7017)
                     /gene="ypdF"
                     /locus_tag="OQM83_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7032..8279)
                     /locus_tag="OQM83_002334"
     CDS             complement(7032..8279)
                     /locus_tag="OQM83_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCS1520585.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(8301..8627)
                     /locus_tag="OQM83_002335"
     CDS             complement(8301..8627)
                     /locus_tag="OQM83_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCS1520586.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(8847..9812)
                     /gene="glk"
                     /locus_tag="OQM83_002336"
     CDS             complement(8847..9812)
                     /gene="glk"
                     /locus_tag="OQM83_002336"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCS1520587.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRATFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            10016..11272
                     /locus_tag="OQM83_002337"
     CDS             10016..11272
                     /locus_tag="OQM83_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCS1520588.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            11387..11713
                     /locus_tag="OQM83_002338"
     CDS             11387..11713
                     /locus_tag="OQM83_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCS1520589.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(11854..13092)
                     /locus_tag="OQM83_002339"
     CDS             complement(11854..13092)
                     /locus_tag="OQM83_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCS1520590.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            13428..14630
                     /gene="nupC"
                     /locus_tag="OQM83_002340"
     CDS             13428..14630
                     /gene="nupC"
                     /locus_tag="OQM83_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCS1520591.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(14680..16868)
                     /locus_tag="OQM83_002341"
                     /pseudo
     CDS             complement(14680..16868)
                     /locus_tag="OQM83_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(17077..17152)
                     /locus_tag="OQM83_002342"
     tRNA            complement(17077..17152)
                     /locus_tag="OQM83_002342"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17117..17119),aa:Ala,seq:ggc)
     gene            17373..17732
                     /locus_tag="OQM83_002343"
     CDS             17373..17732
                     /locus_tag="OQM83_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCS1520592.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            17734..18126
                     /locus_tag="OQM83_002344"
     CDS             17734..18126
                     /locus_tag="OQM83_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCS1520593.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(18178..19593)
                     /gene="gltX"
                     /locus_tag="OQM83_002345"
     CDS             complement(18178..19593)
                     /gene="gltX"
                     /locus_tag="OQM83_002345"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCS1520594.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            19852..19927
                     /locus_tag="OQM83_002346"
     tRNA            19852..19927
                     /locus_tag="OQM83_002346"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19885..19887,aa:Val,seq:tac)
BASE COUNT         4949 a         5331 c         4947 g         4727 t
ORIGIN      
        1 aaaatttact actgccacgc gcatcagatc acctcgtcat tacaggaaat ggcggtcggt
       61 ctgtcgcaaa tcatctccac gcaactggag gtttcacgcg ccgagcagct acgtgaaatg
      121 gcaaataagg cagagcttcg cgccctgcaa agcaaaatta atccccattt tctgtttaac
      181 gctctgaacg ctatttcatc gtcaatccgt ctgaatccgg ataccgctcg ccagttgatc
      241 tttaatctgt cgcgttatct gcgctataac attgaattaa aagacgatga gcaaatcgat
      301 atcaaaaaag agctgtatca aattaaagac tatattgcca ttgagcaggc ccgctttggt
      361 gacaagctga cggttatcta tgatattgat gaagaggtga attgctgcat tcccagcctg
      421 ctgatccagc cgttggtgga gaacgccatt gtccacggta ttcagccttg caaaggtaaa
      481 ggcgttgtca ccatcagcgt tgcagagtgc ggaaatcggg tacgcattgc ggtgcgagat
      541 accgggcacg gcatcgatcc aaaggtgatt gagcgggtcg aagcgaatga aatgccgggc
      601 aataaaattg gcctgctgaa tgtccatcat cgcgtgaagt tattgtatgg cgaggggctg
      661 catatccgcc gcctggagcc ggggacggaa attgcgtttt acattcctaa ccaacgcacc
      721 ccagtcgcct cacaggctac gttattgctt tgagccggag tgatattgtg aaagtcatca
      781 ttgttgaaga cgaattcctg gcacaacagg aactgagctg gctaattaaa gagcacagcc
      841 agatggagat tgtcggcacc tttgacgacg gtctggacgt gttgaagttt ttgcagcata
      901 accgcgtcga cgccattttt ctggatatca atattccgtc gctggatggc gtgttgctgg
      961 cgcaaaacat cagccagttc gcccataaac cgtttattgt gttcatcacc gcgtggaaag
     1021 aacatgcggt agaagcgttt gaactggagg cgtttgacta cattctcaaa ccgtatcagg
     1081 agtcacgtat taccgggatg ctgcaaaaac tggaagcggc ctggcaacaa cagcagacca
     1141 gcagtacgcc tgccgcgacg gtaacgcgtg agaatgacac cattaatctg gtgaaagatg
     1201 agcgaataat cgtcacgcca attaacgata tctattacgc cgaagcgcac gagaaaatga
     1261 cctttgtcta tacgcggcgt gaatcctacg taatgccgat gaacattacc gaattttgca
     1321 gcaaactgcc gccgtcgcat tttttccgct gccatcgctc attttgtgtc aatctgaaca
     1381 aaatacgcga aatcgaaccg tggtttaata acacctacat tctgcgactg aaagatctgg
     1441 attttgaagt gccggtcagc cgcagcaaag tgaaagaatt tcgccagtta atgcatcttt
     1501 aatcgggaac tttcatgaaa gcaccaggct tgcccgccga tcaacaattt ttcgccgatc
     1561 tgttcagcgg cctggtgctt aacccgcaac tactggggcg cgtctggttt gccagccagc
     1621 ctgcctcgtt accggtgggc agtttatgta ttgattttcc ccgtctggat atcgtgctgc
     1681 gcggcgaata cggcaacctg ctggaagcaa agcagcagcg aatggtggaa ggagaaatgc
     1741 tgtttattcc ggcacgcgcg gctaatttac cgatcaacaa caaaccggtg atgctgttaa
     1801 gcctggtgtt cgcaccgacc tggcttgggt tatcgtttta cgatagccgc accacgtcgt
     1861 tgttgcatcc tgctcgccag atccagcttc ccagcctgca acgcggtgaa ggtgaagcga
     1921 tgcttaccgc cctcacccat cttagccgtt cgccgctgga gcagaatatc attcagccac
     1981 tggtgttaag tttgctgcat ctttgccgca acgtggtgaa tatgccgccg ggtaattcgc
     2041 aaccgcgcgg cgattttctc tatcacagca tttgtaactg ggttcaggat aattatgccc
     2101 agccgctcac ccgcgagagc gtggcgcagt tttttaatat cacgcccaat catctgtcaa
     2161 aactgtttgc tcagcatgga acgatgcgtt ttatcgagta tgtgcgttgg gtgcgaatgg
     2221 cgaaggcgag gatgattttg cagaaatatc atctgtcaat tcatgaagtg gcacagcgtt
     2281 gcggttttcc ggatagcgac tatttttgtc gcgttttccg gcgtcagttt ggtctgacgc
     2341 cgggagagta cagcgcccgt tttcagggct aacgtcagaa ggttaattct gtttccagca
     2401 gcgtcaggat actttgtgca tcttgcgcgg caaacaacga ctggcggaag tttttattca
     2461 ccagtttacg cgccagctgc gagaagactt tcacatgatt catcccttcg ttagcaccca
     2521 gcgtcagcat gatcaccagt tcaacttcgc ccatttctga ctgccagtca accggtttcg
     2581 ccagccgggc aatgctgata ctggaatgac ggatccactg agatttggtg tgcgggatcg
     2641 ctacgccaaa accaacgccg gtggtaacaa tctcttcccg ctgccagaca tcttcttcca
     2701 gctcaaacgg atgttcagtg cgcccgttaa cgccgaggtt gccgcacagg aactggatcg
     2761 cctgctcttt attgctaaaa tcctgatcaa caaagatatt ctccagcgcc agcagtgggc
     2821 gaacgtcttc ttccggcgta aaggcggtga gtaacgcttc aatttcctgc gcactgcggc
     2881 attcacatgc ctgacgcgcc agttcccgac acgcctcgct atccagttga cgaagctggc
     2941 ttttcaccgc cggaatacgc gggctactca tactcagctc atccaggccc agcccaagca
     3001 gtagcggcag ataacggctt tcaccgccca gttcaccgca aatgcctacc catttgcccc
     3061 gctgatgcgc agtggtaact atttgctgca acatgcgcag gaacgatggc gtaatcgggt
     3121 tatatagcgg cgatacgcgc gggttattac gatcgaccgc atacagatac tgggtcatat
     3181 cgttggagcc gatactgaag aaatcgacct catcgcagaa gtggtcgatg atgtagcaca
     3241 ccgacggcac ttccaccatg atcccaagcg taatcgtctc tgcatgacgc aggccatcgc
     3301 gcttaagctc aacgatcgct ttttggatct cgcctttcac ccataagatc tgatcgaggc
     3361 tgttaaccat cgggatcatc aactgggcgt tgccgaaact ggcggcgcgc aaaatggccc
     3421 gcagttgagt gcggaacagg ccagcaaatt ccgggtaaat acgtaccgcg cgatagccga
     3481 ggaacgggtt ctcttcctgg ggaatattca gataaggaat gcttttatcg ccgccgatgt
     3541 ccatcgtgcg gaagataatc ggcttgtcgc ccgccgccag tagcacctgc tggtaggctt
     3601 caaattgctc ctgctcgtcc ggcgcgctgt cgcgatccat atagagcatt tcggtacgga
     3661 acagcccgac accttccgcg ccgttggcaa acgcgcctgg cgcttccaga gcggtgccga
     3721 tattcgccgc aatatcaata cgcttgttat cacgggaata ggccagctgc gcggcagcct
     3781 gcgcctgttg tttttggcgt ttatccgcca gcgtctgcgc gacctgataa taaccgctca
     3841 ccgcgtcatt cgggttaatc gccagcacgc cgcactgggc gtcaagcact gcaggttgcc
     3901 cggcataacg ggcaatcgca tccagcggca agccactcag taccgggatc gccgaggcac
     3961 gggccagaat cagtgtatgc gaggtgcgcc cggttttctc cagaatcatg cccgcaagat
     4021 ttttcaaatc aaggctcaaa aactggcttg gggttaaatc ttcagccacc agaatggtcg
     4081 gtttttccag caccagattg ttgcgcggct tcagttccgg ctaggtgata tgcagcaact
     4141 gttcgctgat atcgcgaatg tcgctgaccc gctcgcgcag ataatcgctg gcagaagcag
     4201 atagtttggc gcaaacctgc tccatattgc tgatgatcgc cgcccccagt ccctgatgct
     4261 gttctgtcat caggcgacgg atattgcctg caaattcatc atcctgaatc agcgacaaat
     4321 gagcgctgag gatagttttg ctttcgccgt cacgctcacg caattgctgg ttcagttgct
     4381 cggcaagcgt tgccaggctg tgctccagcc gggtggaatc ttgcgcactg gcggggattg
     4441 cccgataact gtcgaggctg tcgctttgta acagggtcag cgtacccacg ccgacgccgc
     4501 ttgccagcac attgccgtac agtaaatccg ggttaaggcg gcttaatgaa cgcggcagcg
     4561 gatgcgccgt cagttctgcc tgcgtcggct gaacgctgtc gctgtcgata aagcgcacca
     4621 ggatgtactc ttccagcacg cgccgcgcct gctcttcatc gctgccgcta atgttcaggc
     4681 tgcaactgtc attaaataag gtgccagttc caatcaacgc cagcgagctt ttagcatcgg
     4741 cctttgcgtt ctggcgatgg ttgataaagg tgatttcgct ttgccactgg ctgcactgtt
     4801 ctttaagttc ccacgccgga cgagcatgta gaccgttagg caaaggacag agaaattgaa
     4861 tcgttaacat aaccgctccg ggttaatcag gagatcatct gaaatccgtc agttgaacaa
     4921 ccgtctcacg cgtaagacgt tgaataaggg cagataaaag ttgctccatc tgcaaaatgt
     4981 cgcggcaatc ggcaatcgat gccgcgcaat gtccgtggcg ggtggcaggc cccattacca
     5041 ctgtgggtac gccagtatcg gttaagtgca ccgctccacc gtccgtgccg ccgttactga
     5101 acatatccgc ctgcaacgga acgccaattt ctgccgacac ggtttcaatc cacgcggtaa
     5161 gttttggcgg cgcaatcagc gatttatcgc ttaacaccag catcggcccg ttaccaatct
     5221 gacgatggtt agccgcgcca taatcaaagt ttttcgccca gcaggcggta tcaagcacaa
     5281 tggcgacgtc cggcgacacc gcgcgggtag cagtttgccc gccgcgtaat cccacctctt
     5341 cgctggaact tgccaccagc cacacttccg caggcagttc ggcgtcgtgc agctcacgta
     5401 gtaacgtcac cagcagatag caaccgaggc ggtcatcaaa ggctttcccc atcacccgct
     5461 ggtgagggag aacctgaaaa gctgtatcaa aggtgacgcg atcgcctgga cgaattcccg
     5521 cctgcatcac ttcgtcatac gagcgcgcac caatgtccac gcgcatggcg ctgacgtcat
     5581 tcccctgccg gtcgccgtca agcaggcctg gaattttgca ctcttcacgg gtggtgatgc
     5641 gcaccggctg cagctggcgg gcagccatgc gcacgttgcc aaccggcagc acatcaattg
     5701 ccccttcgcg ggagatgctg cgcaccataa atcccacttc gtccatatgc gcacagatca
     5761 tcaccttcgg acctgtcgat tcattgaggc ggatcagcac cgatcccaga ccatcaaatc
     5821 gtacttcttt ttgcaggcga tccgcttctt ccagtaggat ctgccgcact tcctgttccg
     5881 aggaggcgat cgcatctgcc tcgctcaacg cttttaatag cgataaatcc attatgcctc
     5941 tcccgtgagc aacactgttt tcggcatggc gtagagcact tctgcgcctt gcggggtaac
     6001 cagcacaaca tcttcgatgc gcacgccccc ttgccctggc aaataaatcc ccggctccac
     6061 ggtcagtaac atgcctggct gtagcgtcgt ggtatcccgc ggtgaaaagc gtggatcttc
     6121 gtgtacttca ataccgatag catgaccggt gttgtgacca aaatagtcgc caaatcctgc
     6181 ctcggtaatc acccggcgcg cggcgtcgtc aacctgttag cagcgtacgc cagggcgaat
     6241 cgcggagatt gccgccagct gtgcttgcag gacaatctga tagacgttaa acagcgggtg
     6301 agattcggca attacccctt cgccattcac cagcaaggtg cgcgtcatat cagagcagta
     6361 cccctgatag agcgcaccaa aatcaagagt gacaaactcg cctgctgcaa caatcttgtc
     6421 gctggctttg ccgtgcggca gcgccccacg ccagccgctg gcgacaatgg tgtcaaaaga
     6481 ggctttttct gcgccctgct ggcgcataaa ccactccagt tcagcggcta tctcgcgctc
     6541 gctcatcccc gcctgaataa agcggcgaat atgctccgct ccgcgatcgg caatcccaca
     6601 ggcgaggcgg attatctcca cctcctctgg cgttttgatt tgccgcagca catccggcgt
     6661 ggcgctaacc agtttcgcat tgagttcaga cttccagcgg tgcgcggttt cccaactcac
     6721 ctgctggcct tcaaaaccga gcgtttgcaa ctgctcatcg gcaatgattt gattgacgat
     6781 agtggtaagc gtgttcgtcg cgtcaagcaa atgcagctgg tagccttgcg cacgggcttc
     6841 cacctcaacg aaatagcgcg aatctaccag aatgtgcgca ctttcacggc taataaccac
     6901 atagcctgat ccggtggaga tccccagatg cggctgtttg ttctgccgtg aggagagaag
     6961 cactgcatcc agttgttgcg ccttaagcca gtcgcgcagc gaagcgagta atgtcatctg
     7021 gattttcctt cttacaggct atcgattaac aatttacctt tacggaacat catcagacgc
     7081 aggaacacca ccatcagcgc agttatgact gcccccagcg cgatccccgc catatagacg
     7141 cccaggttag tgaccagcgg ccatgcccag atagcggatt ccgggaacca ttgcactgcg
     7201 cccagccaga cagcggcggt tgagccgaca atcgcgccga ccatatacga cggaatggcg
     7261 gtgatggggc tttccagcgc aaacggaatc gcaccctcac taatccccat aaaggcaagg
     7321 aacatcgcgg tcttaccctg cggataaagt tgagcgttga acaggcgttt gccggttaaa
     7381 cgacggtcga taatggtcgc cagacccaga cctatcggcg gaataacgat agcgatagaa
     7441 cgtgcagtga ccggtaatac gtggtcagtg gtaaagctga aggcaacaaa acctgcggct
     7501 ttgttgatcg ggccaccaag gtcgattgcg gtcgccgcgg cgatccccat tgcgtacatc
     7561 agcgcgccct tctcacctgc ggcagtcagc acggtacgga taccgccgtt gatccagccg
     7621 ccaaaggggg taatgacgta gtacatcgcc agcatgacaa aaatggcgga aagaatcggt
     7681 agcaaaaatg tggttttgaa cgctaacagg aaatccggta actggatttt ctggttcatc
     7741 cacttcacaa ggtaacccgc gacgatagaa ataatcagcg caccaatgaa ggtggacggc
     7801 accggcgaag aggtcgccca ttgcatagtg ctgggatcga agttcagcag ttgcgtcggc
     7861 tgggtagaca tcaagccgcc gataaaacca gccgggaacg ccagtttgcc accgatagag
     7921 ttcgccacaa aagcagcaaa catcggaatg gcaaaaccaa acaacacgcc gccgaaggac
     7981 tgcgacagcc aggcaaattt cagtaaggag aggtcgaaac cggagaattt cccgctatta
     8041 agagcatcca tgatgccgat ctcagcagga attttaagcc agctataagc aatcagctgg
     8101 ctgaaagcga ggatcacacc gcccataatt aaggtcggca ccatgcgtga aatccctgac
     8161 atcacatgct gcgggagttc accccagaag ctgcttttag aggcctgcgg attcttaact
     8221 gccgctgccg caccggatgc gccaggcaca acggttgcac tgcgtttttt aatggccata
     8281 acaattatcc ctgtcgaata ttactgttgt tcagaggcaa tcatctcttc gatttctttg
     8341 atgatgcccg cagcgttttt aattgcgtcc tgcaaagtga tttcataaac gtcgcgtgat
     8401 tcgaaacgct cattatcttc cggggtaact gccacggagt ggatgatgat ggttgcttcg
     8461 gcgatatcct gcgccgtcag gcgattctgg ataccgtccg cgccctgggt ttcaattttc
     8521 acttcataac ctgcttctac cgccgcttct tccagcgcct gagcggccat aaaggtgtga
     8581 gccaggccca ttgggcaggc acataaggca attagtttct tactcatcgt taattcctcg
     8641 ttcagtgata gttgacgagt gaaattatga acgtgcaaaa caaatcgccg ttaccagaca
     8701 aataatgcat ttactggaga attaatcccg ctgtacggaa gtgtgacgca ggtcggcctt
     8761 gtgaggggga cacaaagaaa aattgaatta actgtatgat ttaaaagatt atcgggagag
     8821 ttacctcccg atataacaga aaggatttac agaatgtgcc ctaaggtctg gcgtaaatgt
     8881 gcaccggaac cgagaagacc cggattgtca tggacgatga gatacaccgg aatatcatgg
     8941 acatattctt taaagcgccc tttatcttca aatgtggcac ggaaaccaga ggctttgaag
     9001 aactcaagga agcgcggcac gataccgcca gcaatataaa ccccaccaaa ggttcccagc
     9061 gttaatgcca gattgccgcc aaaacggccc ataatgacgc aaaacagcga caacgcacgg
     9121 cggcaatcgg tgcagctgtc agccagcgcg cgttcggtaa tatcttttgg cttgagattt
     9181 tctggcaggc ggttgtcggc tttaacaatt gcgcgataca aattcaccag cccaggacca
     9241 gaaagcacgc gttccgccga aacatgacca atttccgcac gcaatatttc gaggataatg
     9301 gcctcttctt cactattcgg cgcaaaatca acgtgaccgc cttcgcccgg caagcttacc
     9361 caacgcttat cgacatggac cagatgcgca accccaagcc ctgttccggc accgtaaacc
     9421 gcaataggtt taccttcgac cggttctgcg ccaccaaact gaatcagatg ctcttttttc
     9481 agcatcggga tcgccatcga tacagcggta aaatcgttaa taatttccag atggctaaaa
     9541 ccgagattct ttttcatttc ggcaattgag aacgcccagg tatggttggt cattgccacc
     9601 cagtcaccgg taattgggca agcgatggca atacagccgt ctttcacctc gaccttatgt
     9661 tcttcaagat aaacgcgaat gaccgcttcg aggctggggt aatcaagccc tgaataggtc
     9721 ttagcctgcg agatttcacc actggcaata tcacacagag caagacgtgc gttggtgccg
     9781 cccacatcac cgactaatgc atactttgtc attcttcaac tgctccgcta aagtcaaaat
     9841 aattctttct cacactgtaa atacctgggg gcataacaac aacgccggaa aggcaggctc
     9901 cctgtaaata tcgatctggg tcacacattt actttatcgt ttcagcacca attgcagcga
     9961 tgcctttttg caagctgggc aaactaagta tctgaccccg cataaggaat agaacatgct
    10021 ccatccgcga gccagaacca tgttgttatt atcgctcccc gccgtggcaa ttgggattgc
    10081 gtccagtctt attctgattg tggtgatgaa aatcgcctcg gtattacaga atttgctctg
    10141 gcaacgactg ccgggaactc tggggatagc ccaggattca cctatctgga tcatcggtgt
    10201 attaacgcta acgggtattg cggtggggtt ggttatccgt ttcagccagg gtcatgccgg
    10261 accagacccc gcctgtgaac cgctgatcgg cgcaccagtt ccgccctctg cgctaccagg
    10321 acttatcgta gcattaattc tcggtcttgc tggcggcgtc agcctggggc cggaacatcc
    10381 gatcatgacc gtcaatatcg cccttgccgt cgccattggc gctcgtctgt taccgcgcgt
    10441 caaccgaatg gagtggacta ttttagcctc tgccggaacc atcggcgcgc tgtttggtac
    10501 acctgttgcg gcggcgttga tattttcgca aaccttaaat ggcagtagtg aagttccgct
    10561 atgggatcgt ctttttgcgc cgttaatggc ggcagcagct ggtgcactta ctaccggatt
    10621 atttttccat ccccattttt cactgcccat tgcgcattac ggacagatgg aaatgaccga
    10681 tattctcagc ggtgcaattg tcgcggcgat tgccatcgca gcagggatgg tcgccgtatg
    10741 gtgcttacca cggttgcacg cgatgatgaa tcaaatgaaa aatccggtgc tcgtgctggg
    10801 tattggcgga tttattctcg gaattctggg ggttattggt ggaccagttt cgctgtttaa
    10861 agggctggat gagatgcagc agatggtggc aaatcaggct ttcagcacca gcgattactt
    10921 tttgctggcg gtaattaaac ttgccgccct ggtcgttgct gccgccagtg gctttcgcgg
    10981 tgggcgaatc ttcccggcag tgtttgtcgg cgtggcatta gggttgatgc tgcatgagca
    11041 cgttcccgcc gttccagcgg caataaccgt ttcctgcgct attctcggca tcgtgctggt
    11101 ggtaacacgc gatggctggt taagtctttt tatggcggca gtcgttgtac ccaataccac
    11161 attgctaccg ctgctctgta tcgtcatgct tccggcatgg ctgttattag caggtaagcc
    11221 gatgatgatg gtcaatcgtc cgaagcaaca gccaccccac gataatgttt agcaaaaaat
    11281 gctctccttt atgatttaag agttatggcg atgatacggg gctttacctc cccgtaatat
    11341 tgcgttaaca ggccgctgac ggcttcgatc gtgaaggaga ataacaatgt tcaggtcact
    11401 gtttctggcg gccgccctga tggcatttac cccgcttgca gcaaatgcag gtgaaatcac
    11461 cctactgcca tcgatcaaat tacaaattgg cgatcgcgat cattacggta attactggga
    11521 cggtggtcac tggcgagatc gtgattactg gcatcgcaat tatgaatggc gcaaaaaccg
    11581 ctggtggcgt catgataatg gctatcatcg tggctgggat aagcgtaaag catatgagcg
    11641 tggctatcgt gaaggctggc gcgatcgtga cgatcatcgc ggaaaaggcc gcggacatgg
    11701 gcaccgccat taaacgcgtc ctccaatgga gcacaatgcc tgatgcgacg ctagcgcgtc
    11761 ttatcaggcc tacaaaccgg ctctttaacg cgtagggcgg ataaggcgtt tacgccgcat
    11821 ccgccaatag tgccagatgc gacgctcatt caactacaat cccagcgccg tccccaccaa
    11881 caaccagata ttcagcgcca cgaccagcac tacaatcacc cagcctgtct gttttacgcg
    11941 tttgctgttc accagatcgc ccatcaactt gctgtcactg gtgaaaatca gcagtggaac
    12001 cagcgccagg gcgataccaa aacttaacag cacctgactc ataaccagaa tccgtgtcgg
    12061 atctaatccc atcagaatga caataaatga cggcaacatg gtgactgtac gacgcaccca
    12121 cagcgggata tggaagcgaa tgaatccctg catcaccacc tgccccgcca gtgtccccac
    12181 caccgttgag gacagtccgg cagcaaccag acttaaccca aagaccgttg ccgcagcatg
    12241 gcttaacagc ggttgcagcg tcagataagc ctcatcaaga tcggcaacac cagtatgacc
    12301 agaaaagtgg aacgccgccg cagctgtagc catcatcgcc agattgacaa aaccggcaat
    12361 cgtcatggcg atagccacat cccatttggt ggcggaataa cgttgttgac gcgaaccgcc
    12421 atgtaaatgc tgagtgagcg aagagtgcaa ataaatcaca tgcggcataa tcgtcgcccc
    12481 taacacgcct gctgccagga agaccgcctc cgaagtaggt aaactcggga tcaccattcc
    12541 tttacccagc tgcgccaggt taggctggga gaaaatcaac tcgacaatgt aagccgcggc
    12601 aacaaacaac agtaacccgc caatcacttt ctccagtggt ttttgcccgc gacgttgcag
    12661 cattaaaatc aggaaagtcg cgatccccgt cagcaccgcg ccctgcaaca acgagacacc
    12721 aagaatgagt ttaaaaccga tcgccgcacc aataaattcc gccaggtcgg ttgccatcgc
    12781 aataatttct gcctgaaccc aatagaacca cactacggga cgcggatagt gatcacgaat
    12841 ctgctccgcc agattttttc cggtggcaat ccctagtttg gcagagagga tctgaatcag
    12901 catcgccatc aggttggccc aaacgacaac ccacagtagc tgatagccga agctggcacc
    12961 cgcctgaata ttggtcgcaa agttaccggg atcgatataa ccaatcgccg caatgaacgc
    13021 aggtcccatt aatgcgagcc tcatcttgcg cgccgcccgt ccgctgctac tctcaacgcg
    13081 atagttcgtc atcttgtgcc tctaaaacat agcctttgct atgtttcatg ctatgataaa
    13141 cgagaatgat tatcaaattc atttaaatgg attgtggtga tttctctgat agaccaggat
    13201 tatgactacg aaaagattga tgacttgaat gttatggtta tgtttaatgt tagcacattt
    13261 acataacttt cagcttccat acacaacata gcagaaatgt atgacagatc actatttttg
    13321 aagcctgtca caggacgtca ttatagtgtg tgtcagatct cgttttcctt aaccatgtta
    13381 catagaatgt gcacggaaat ttaacctgcc tcatatttgg agcaaatatg gaccgcgtcc
    13441 ttcattttgt actggcactt gccgttgttg cgattctcgc actgctggta agcagcgacc
    13501 gcaaaaaaat tcgtatccgt tatgttattc aactgcttgt tatcgaagtg ttactggcgt
    13561 ggttcttcct gaactccgac gttggtttag gcttcgtgaa aggcttctcc gaaatgttcg
    13621 aaaaactgct cagatttgcc aacgaaggga ctaacttcgt ctttggtagc atgaatgatc
    13681 aaggcctggc attcttcttc ctgaaagtgc tgtgcccaat cgtctttatc tctgcactga
    13741 tcggtattct ccagcacatt cgcgtgttgc cggtgattat ccgcgcaatt ggtttcctgc
    13801 tctccaaagt caacggtatg ggcaaactgg aatcctttaa cgccgtcagc tccctgattc
    13861 tgggtcagtc tgaaaacttt attgcctata aagatatcct cggcaaaatc tcccgtaatc
    13921 gtatgtacac catggctgcc acggcaatgt ccaccgtgtc gatgtccatc gttggtgcat
    13981 acatgaccat gctggaaccg aaatacgtcg ttgctgcgct ggtactgaac atgttcagca
    14041 cctttatcgt gctgtcgctg atcaatcctt accgtgttga tgccagtgaa gaaaacatcc
    14101 agatgtccaa cctgcacgaa ggtcagagct tcttcgaaat gctgggtgaa tacattctgg
    14161 caggtttcaa agttgccatt atcgttgccg cgatgctgat cggctttatc gccctgatcg
    14221 ccgcgctgaa cgcactgttt gccaccgtta ctggctggtt tggctacagc atctccttcc
    14281 agggcatcct gggctacatc ttctatccga ttgcatgggt gatgggtgtt ccttccagtg
    14341 aagcactgca agtgggcagt atcatggcga ccaaactggt ttccaacgag ttcgttgcga
    14401 tgatggatct gcagaaaatt gcttccacgc tctctccgcg tgctgaaggc atcatctctg
    14461 tgttcctggt ttccttcgct aacttctctt caatcgggat tatcgcaggt gcagttaaag
    14521 gcctgaatga agagcaaggt aacgtggttt ctcgcttcgg tctgaaactg gtttacggct
    14581 ctaccctggt gagtgtgctg tctgcgtcaa tcgcagcact ggtgctgtaa aaccatacat
    14641 aaaaaagccg gggatcattc cccggctttt ttacgcctgt taatcagcta atggctgcgg
    14701 gcgaccaatc aaataccctt gcagatattg caccccgagc ttatgcaata gcgcctgctg
    14761 ctgttgcgtc tcgacaaact ccgcgaccac actcaatgac ttcgctttcg ccagatcggt
    14821 aattgatctc acaatcatcg catccagcgt attcgtgaca atatctttca caaagacgcc
    14881 atcaattttg atgatatcag cctgcaaacg ctttaaccgt tcgtagttgg catatccggt
    14941 gccaaagtca tcaatcgcaa tccggaaacc aaacttatgc agctgctcga tgttgtacat
    15001 gctgctttct gcgttagaaa acgcctgctc ctcggtgatc tcaagaatga ccgcctgcgg
    15061 ggagatgtga taacgcttaa acagacgaat aatccgcccg gcaatattct tttgcagcag
    15121 cgtgagcggc attaaattga ctgaaaagcg cggtcctttt ttgtcgcaag ggtgtgttgc
    15181 cagccacttc aacaaggatt ccagcacttg caaatcaaaa cgcgcgctaa ggttaaactg
    15241 agcaataagg ggcagaaact tatccggggt cataatgccg ccgtcatatt tcagtcgcgc
    15301 gaggatctca tcataacctt caccctcttt gttgcgaatt ggctgggcgt agagcagcaa
    15361 atcaccctga tctaacgccg tgcgaatggt attcagcaat agcacctgtt tagtggtctg
    15421 cccggaaacc atctcctctc tgctatccag cgccagcaca tgatgatgtg cgcaggattg
    15481 ctccgccagc cagcttaact gccccaacaa gggttgcagg gtttcctgat ttccatcaaa
    15541 acgcccccag gcagcaccat agcccatatc cagcccggta ttgttccagt gaatttgccg
    15601 actattcagg atgttaacca tatgctggag tcgcccttcc gtttccggcc cgctcagcac
    15661 taacagcagt tcactacccg gcaattgata taacttttcg ttttcctgca tcaacggcag
    15721 cagcgtacgg taaattgatc ggatacagtg aacgcgcatc attaagccgt aatgacgact
    15781 cataaactca agattatcaa tgcgcaggca gcaaaactct tgcccgcctc ttgctccggc
    15841 gcttgttcca acgcacggaa gttgggcagg agcgttaacg gatcggtcag cgcctgcaaa
    15901 tgccagcggc gattaagcca ttcactgcga tgataaatgc gcaccatata gagcaggcaa
    15961 acgctaaagg aaatcagcac cgcaagaata aatgccagcg aatattcggt ttccacccct
    16021 tgcaaaaagt tctggttgta attcagaagg caaagcgttg aaacagccct ggtgagattt
    16081 aaaaacggat agcgaagctt accgaccccg agggtaaaga tgatgaagaa aacgggtacc
    16141 aggtaaccgg caataaagtc gttttcataa ggtgtgcaca acagaagtag aagcacgctt
    16201 aacgctgcca gccagcttaa ggtaaatgcg cgtttctctt tgcccaacga cggcgcgata
    16261 tccctgcgcc acaatatctg cgcaaagtgg ggacttacaa tcatgcgagt gagatagtag
    16321 aaaagcatgt tgtaaataag cacagcggtg aaaaggctta gcaaatcaac gaccgtgaaa
    16381 atggcatccg catcgccgaa aaaggtggat atctttagcg ggaaatcaaa gaagctcccc
    16441 acaagataca tgctgcattt gatgccgatc ggcgtcacca aaccaagcca gaacagacgt
    16501 tgccagacat atcggctggt caatccataa cgccaacgcg tccccagctg ccagcgtaat
    16561 attgcgcagg cacacaacac gacaaacgtc tggctaccga gcatgaccgc agtctgtaaa
    16621 aaagagagat gaaagttcca cagattggtg caaaacatcc cgcataaaac gggaacgaca
    16681 ccgcgccagc caaagataaa caagattgac agcattacgc acagtggcat ccacgccaga
    16741 aagatataac tggaatggat aatggcaagt ggcgaaataa aacgggatag ctgaataagt
    16801 accacggtga gcgtaaacgc tagtgtgaat atcttgatat tttttatcag gttatgctcc
    16861 acaaacatga gtacactact ctcactgatc ggggttttca ggcacacaaa taataagttt
    16921 attcgcatcg cgaagcaaac agggggccag cctgttgccg atttttcatc caatgatgct
    16981 ttcaccaggc ggcactaacc cggacgtaga ctgtattaca aaagcggcaa aaagcagaga
    17041 caaaaaaccc ccgctttgca gcgagggttg gaaatttggt ggagctaagc gggatcgaac
    17101 cgctgacctc ttgcatgcca tgcaagcgct ctcccagctg agctatagcc ccgtcacgta
    17161 aagcttgtcg agttgacggg cggcatcata tgaattccgc ccgaatgtgt caacggcaaa
    17221 ttgcaacgtg tagtttcaat cgctgaaaaa tcaggcaaat gaacaatttt ggaaaaccgc
    17281 tcgcattcag tagttattca tgtcacggtt tcctgtaaag tggtgttata aaatgaacta
    17341 ctaatagacc cacatacatt cagggaattg ttatgttcaa ggagaggatg acaccagatg
    17401 aacttgccag actgaccggc tatagccgcc agaccattaa taaatgggta cgcaaggagg
    17461 gctggacaac gtcaccaaaa cctggcgtcc agggtggcaa agcccgactg gttcacgtca
    17521 atgaacaggt tcgtgaatat attcgcaatg ctgaacgtcc agaaggtcag ggagaagcgc
    17581 ctgccctttc tggtgatgcg ccgcttgaag tattgctggt gacactggcg aaagagatga
    17641 cgccagttga acaaaaacag tttacatcct tgcttctgcg ggaagggatt atcggattgt
    17701 tacaacgctt agggattcgc gatagcaaat aatatgaaaa gattacgcaa taaaatgacc
    17761 accgaagaac tggctgaatg cctcggtgtg gctaaacaaa ctgttaaccg ttggatcaga
    17821 gaaaaaggct ggaaaacgga aaaatttccc ggcgtgaaag gcggtcgtgc cagacttatt
    17881 ctggtcgata cgcaagtttg cgagtttatt cagaacacgc cagccttcca taacacacca
    17941 atgttgatgg aagctgaaga gagaattgct gaatacgctc ctggagcacg cgccccggcg
    18001 tatcgacaaa tcatcaacgc gatagataat atgacagaca ttgagcaggg aaaagtcgcg
    18061 caatttttgt cacgtgaagg aattcgcaat ttcctcgccc gtctcgatat tgacgaatca
    18121 gcataaataa aaaacggcag gatattatct cctgccgttt atctttttac acgctaatta
    18181 ctgctgattt tcgcgttcag caataaaatc cagcgctttg ttgatacgct cgatactgcg
    18241 ggtcttacca attgcgtgaa cggtaacatc cagtgctgga gactgccccg ccccggttac
    18301 ggcgacacgc agcggcatac caactttacc catacccact tccagctcat ccgccgtcgc
    18361 ctgaatagcg tgatgaacgt tttcagcggt ccagtcagta atcgcggcca gtttgtcacg
    18421 aaccacttcc agcggctgac gcgctaccgg acgcagatgt tttttcgccg catcggcatc
    18481 gaactcagca aaatcttcgt agaaataacg gcagctctgt gccatctctt tcagcgtctt
    18541 gcagcgttcg cccagcagtt tcaccagatc agccagctgc gggccgttac gggtatcgat
    18601 attttcctgc tcaatgtgcc actgtaagtg agtagcaaca tactccggcg gcagcgcgtt
    18661 aatgtagtga tggttcagcc acagcagctt gtcggtgttg aacgcactgg cagatttgct
    18721 gacggcattc aaagtgaagt atttgatcat ctcttcacga gtgaagattt cctgatcgcc
    18781 gtgggaccag cccagacgca ccagatagtt cagcagtgct tctggcaaat aaccgtcatc
    18841 acgatactgc attacgctga ctgccccgtg acgtttggac agttttttac cgtcatcgcc
    18901 attgatcata gaaacgtgcg cgtaaaccgg caccggcgct ttcagggcct taagaatgtt
    18961 gatctggcgc ggcgtgttgt tgatatggtc ttcgccacgg ataacgtggg tgatttccat
    19021 atcccagtca tcgacaacca cacagaagtt ataggttggg gaaccatcgg tacggcggat
    19081 aataagatcg tccagttcct ggttgctgaa ctcgatcgga ccacggatct gatcgtcaaa
    19141 aacaacagaa ccttcctgcg ggttagcaaa acgtacaaca cacggttcat catcagcatg
    19201 atgctcatgg ctgtggcggc agcgaccgtc ataacgcggc ttctcacctt tcgccatttg
    19261 ctcttcgcgc agcgcttcca ggcgctcttt agagcaatag catttataag cagtgccctc
    19321 ttccagcatc tgatcgatca ccgcgttgta gcgatcaaaa cgtttggtct ggtagtacgg
    19381 accttcatcc cactccaggc tcagccagtt catgccatcc ataatggctt cgatagcttc
    19441 cggcgtggaa cgctcaagat cggtgtcttc aatacgcagc acgaactcac cgccgtggtt
    19501 acgtgcaaaa agccaggagt aaagagcagt acgcgcgccg ccaacgtgca gatagcctgt
    19561 tgggcttggc gcgaagcgag ttttgatttt catgaaatgg ccttacgttt agaaagatgc
    19621 cgacaaccgg caaatcctgg aaaaattgag tgggcgatat tctatcactc ccgcctgatt
    19681 cctcaatgta gttcgggttt ctaccttacg cattattgtt ttcgttgaca aattgcgcaa
    19741 cacggtctgt tttgcgatca tcttgcttaa atttacgacg aacgaacaat ttctttagaa
    19801 aatgcgttga ctcattttga actctcccta taatgcggct ccacacagcg ggggtgatta
    19861 gctcagctgg gagagcacct cccttacaag gagggggtcg gcggttcgat cccgtcatca
    19921 cccaccaact actttatgta gtctccgccg tgta
//
LOCUS       DAJVQG010000075        19094 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJVQG010000075 DAJVQG010000000
VERSION     DAJVQG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19094
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.075"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            271..837
                     /gene="yqaB"
                     /locus_tag="OQM83_002347"
     CDS             271..837
                     /gene="yqaB"
                     /locus_tag="OQM83_002347"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCS1520595.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            834..1262
                     /locus_tag="OQM83_002348"
     CDS             834..1262
                     /locus_tag="OQM83_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1520596.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1335..2891
                     /gene="gshA"
                     /locus_tag="OQM83_002349"
     CDS             1335..2891
                     /gene="gshA"
                     /locus_tag="OQM83_002349"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCS1520597.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            3041..3556
                     /gene="luxS"
                     /locus_tag="OQM83_002350"
     CDS             3041..3556
                     /gene="luxS"
                     /locus_tag="OQM83_002350"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCS1520598.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3620..5158)
                     /gene="emrB"
                     /locus_tag="OQM83_002351"
     CDS             complement(3620..5158)
                     /gene="emrB"
                     /locus_tag="OQM83_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCS1520599.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTKRRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5175..6347)
                     /gene="emrA"
                     /locus_tag="OQM83_002352"
     CDS             complement(5175..6347)
                     /gene="emrA"
                     /locus_tag="OQM83_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HCS1520600.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6474..7004)
                     /gene="emrR"
                     /locus_tag="OQM83_002353"
     CDS             complement(6474..7004)
                     /gene="emrR"
                     /locus_tag="OQM83_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCS1520601.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(7095..7430)
                     /gene="ygaH"
                     /locus_tag="OQM83_002354"
     CDS             complement(7095..7430)
                     /gene="ygaH"
                     /locus_tag="OQM83_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCS1520602.1"
                     /translation="MSYEVLLPGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(7420..8157)
                     /gene="ygaZ"
                     /locus_tag="OQM83_002355"
     CDS             complement(7420..8157)
                     /gene="ygaZ"
                     /locus_tag="OQM83_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCS1520603.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8281..9465)
                     /locus_tag="OQM83_002356"
     CDS             complement(8281..9465)
                     /locus_tag="OQM83_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1520604.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(9756..10748)
                     /gene="proX"
                     /locus_tag="OQM83_002357"
     CDS             complement(9756..10748)
                     /gene="proX"
                     /locus_tag="OQM83_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCS1520605.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGIFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(10806..11870)
                     /gene="proW"
                     /locus_tag="OQM83_002358"
     CDS             complement(10806..11870)
                     /gene="proW"
                     /locus_tag="OQM83_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCS1520606.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(11863..13065)
                     /gene="proV"
                     /locus_tag="OQM83_002359"
     CDS             complement(11863..13065)
                     /gene="proV"
                     /locus_tag="OQM83_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCS1520607.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(13421..14380)
                     /gene="nrdF"
                     /locus_tag="OQM83_002360"
     CDS             complement(13421..14380)
                     /gene="nrdF"
                     /locus_tag="OQM83_002360"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCS1520608.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(14390..16534)
                     /gene="nrdE"
                     /locus_tag="OQM83_002361"
     CDS             complement(14390..16534)
                     /gene="nrdE"
                     /locus_tag="OQM83_002361"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCS1520609.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(16507..16917)
                     /gene="nrdI"
                     /locus_tag="OQM83_002362"
                     /pseudo
     CDS             complement(16507..16917)
                     /gene="nrdI"
                     /locus_tag="OQM83_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            complement(16914..17159)
                     /gene="nrdH"
                     /locus_tag="OQM83_002363"
     CDS             complement(16914..17159)
                     /gene="nrdH"
                     /locus_tag="OQM83_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCS1520610.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(17407..17736)
                     /locus_tag="OQM83_002364"
     CDS             complement(17407..17736)
                     /locus_tag="OQM83_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCS1520611.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            17888..18232
                     /locus_tag="OQM83_002365"
     CDS             17888..18232
                     /locus_tag="OQM83_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCS1520612.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(18269..18718)
                     /gene="alaE"
                     /locus_tag="OQM83_002366"
     CDS             complement(18269..18718)
                     /gene="alaE"
                     /locus_tag="OQM83_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCS1520613.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
BASE COUNT         4794 a         4814 c         4932 g         4554 t
ORIGIN      
        1 aaccttcagc aatgaaggta tcgaagagat agcggtatta accgcaggca ccaggaagga
       61 taacgttgcg ccagcaacgg cccgaagggc gaacgtcagt gagtcatcct cccggatgca
      121 ccatcttctc caaaaaattt ccttcaaaaa tatctcattt ctaaataact cctttatcca
      181 tttcctgaat tttcttctcc ttcgccatca gcgacaaaat caagcaatta cgctaaagta
      241 gcgtcatatt tttcgacttg cgagaagacc atgtacgagc gttatgcagg tttaattttt
      301 gatatggatg gcacaatcct ggatacggag cctacgcacc gtaaagcgtg gcgcgaagta
      361 ttagggcact acggtcttca gtacgatatt caggcgatga ttgcgcttaa tggatcgccc
      421 acctggcgta ttgctcaggc aattattgag ctgaatcagg ccgatctcga cccgcatgcg
      481 ttagcgcgtg aaaaaacaga agcagtaaga agtatgctgc tggatagcgt cgaaccgctt
      541 cctcttgttg atgtggtgaa aagttggcat ggtcgtcgcc caatggctgt aggaacgggg
      601 agtgaaagcg ccatcgctga ggcattgctg gcgcacctgg gattacgcca ttattttgac
      661 gccgtcgtcg ctgccgatca cgtcaaacac cataaacccg cgccagacac atttttgttg
      721 tgcgcgcagc gtatgggcgt gcaaccgacg cagtgtgtgg tctttgaaga tgccgatttc
      781 ggtattcagg cggcccgtgc cgcaggtatg gacgctgtgg acgttcgctt gctgtgagtg
      841 aagcgttatc gcttttctcg ttgtttgcca gtagttttct cagcgctaca ctattacccg
      901 gcaactccga agtcgtgctg gtggcaatgt tgctttccgg gatcagtcat ccctgggttt
      961 tagttttaac agcaacaatg ggtaatagcc ttggagggtt aactaacgtt atccttgggc
     1021 gtttctttcc attgcgtaaa acatcgcgct ggcaagagaa agctatcggc tggctgaaac
     1081 gctatggtgc agtcacgcta ttattaagct ggatgcccgt ggttggcgat ttactgtgtc
     1141 tgttagcggg atggatgcgc atctcgtggg gaccggtaat cttttttttg tgccttggta
     1201 aagcgttacg ctatgttgca gttgcagcag cgaccgttca gggcatgatg tggtggcact
     1261 aattgtaggc ctgcacatat ggtcaccatt acagttatgc taattaaaac gattttgaca
     1321 ggcgggaggt caatttgatc ccggacgtat cacaggcgct ggcctggctg gaaaaacatc
     1381 ctcaggcgtt aaaggggata cagcgtgggc tggagcgcga aactttgcgt gttaatgctg
     1441 atggcacact ggcaacaaca ggtcatcctg aagcattagg ttccgcactg acgcacaaat
     1501 ggattactac cgattttgcg gaagcattgc tggaattcat tacaccagtg gatggtgata
     1561 ttgaacatat gctgaccttt atgcgcgatc tgcatcgtta tacggcgcgc aatatgggcg
     1621 atgagcggat gtggccgtta agtatgccat gctacatcgc agaaggtcag gacatcgaac
     1681 tggcacagta cggcacttct aacaccggac gctttaaaac gctgtatcgt gaagggctga
     1741 aaaatcgcta cggcgcgctg atgcaaacca tttccggcgt gcactacaat ttctctttgc
     1801 caatggcatt ctggcaagcg aagtgcggtg atatctcggg cgctgatgcc aaagagaaaa
     1861 tttctgcggg ctatttccgc gttatccgca attactatcg tttcggttgg gtcattcctt
     1921 atctgtttgg tgcatctccg gcgatttgtt cttctttcct gcaaggaaaa ccaacgtcgc
     1981 tgccgtttga gaaaaccgag tgcggtatgt attacctgcc gtatgcgacc tctcttcgtt
     2041 tgagcgatct cggctatacc aataaatcgc aaagcaatct tggtattacc ttcaacgatc
     2101 tttacgagta cgtagcgggc cttaaacagg caatcaaaac gccatcggaa gagtacgcga
     2161 agattggtat tgagaaagac ggtaagaggc tgcaaatcaa tagcaacgtg ctgcagattg
     2221 aaaacgaact gtatgcgccg attcgtccaa aacgcgttac ccgcagcggc gagtcacctt
     2281 ctgatgcgct gttacgtggc ggcattgaat atattgaagt gcgttcgctg gacatcaacc
     2341 cgttctcgcc gattggtgta gatgaacagc aggtgcgatt cctcgacctg tttatggtct
     2401 ggtgtgcgct ggctgatgca ccggaaatga gcagtagcga acttgcctgt acgcgcgtta
     2461 actggaaccg ggtgatcctt gaaggtcgca aaccgggcct gacgctgggt atcggctgcg
     2521 aaaccgcgca gttcccgtta ctgcaggtgg gtaaagatct gttccgcgat ctgaaacgcg
     2581 tcgcgcaaac gctggatagc attaacggcg gcgacgcgta tcagaaagtg tgtgatgaac
     2641 tggttgcctg cttcgataat cccgatctga ctttctctgc ccgtatctta aggtctatga
     2701 ttgatactgg tattggcgga acaggcaaag cgtttgcaga agcctaccgt aatctgctgc
     2761 gtgaagagcc gctggaaatt ctgcgcgaag aggattttgt agccgagcgc gaggcatctg
     2821 aacgccgtca gcaggaaatg gaaaccgctg ataccgaacc gtttgcggtg tggctggaaa
     2881 aacacacctg acagaaaaga aaaaggccac tcgtgagtgg ccaaaatttc atctctgaat
     2941 tcagggatga tgataacaaa tgcgcgtctt tcatatactc agactcgcct gggaagaaag
     3001 agttcagaaa atttttaaaa aaattaccgg aggtggctaa atgccgttgt tagatagctt
     3061 cacagtcgat catacccgga tggaagcgcc tgcagttcgg gtggcgaaaa caatgaacac
     3121 cccgcatggc gacgcaatca ccgtgttcga tctgcgcttc tgcgtgccga acaaagaagt
     3181 gatgccagaa agagggatcc ataccctgga gcacctgttt gctggtttta tgcgtaacca
     3241 tcttaacggt aatggtgtag agattatcga tatctcgcca atgggctgcc gcaccggttt
     3301 ttatatgagt ctgattggta cgccagatga gcagcgtgtt gctgatgcct ggaaagcggc
     3361 aatggaagac gtgctgaaag tgcaggatca gaatcagatc ccggaactga acgtctacca
     3421 gtgtggcact taccagatgc actcgttgca ggaagcgcag gatattgcgc gtagcattct
     3481 ggaacgtgac gtacgcatca acagcaacga agaactggca ctgccgaaag agaagttgca
     3541 ggaactgcac atctagtcag taaactatct tcacaattaa ttgaaaaaag ccagttcaaa
     3601 tgaactggct tagttgtact tagtgcgcac cgccgccacc gccacctgcg ccaaatggcg
     3661 gtttagcaaa ccacaccagc cccagcagta cgaggaatat cccggctgac atccagaaga
     3721 tctcattggc ggaaataatc agcccctgat tggtgatctg ctgggcaatc cagcctgatg
     3781 cctgctgttg cgtcatccca agcccttcca gttgactgta catcgcctgg gcattcgggt
     3841 tgaacgggtt taccgactca gtcaactgcg catggtgcat cgactcgcgg ttggtccaca
     3901 tggtcgtggt tatcgacgtg ccgatagacc ccgccagcgt tcgcgtaaag ttagagaggc
     3961 tcgatgccgc cgccagtcgt tccggtggca aaccagacag cgtaatggtg gtcagcggca
     4021 taaagaagca ggccaccgca aacccctgga taaactgcgg ccaggccgac gcgccaaaat
     4081 ccatacctgg ttcaaaggta taggcacgcc agtagaagca gacggcatac ataataaagc
     4141 tgaaggttac cagccgccgc atatccagtt tatgcgcgaa gcggccgata atcggcgaca
     4201 ggatcaccgg aataatccct accggcgcag aggccaaacc tgcccaggtc gccgtgtaac
     4261 cgtagacctc ctgcaacaac tgcggcagca gaacaatagc gccgaagtag agcatatacg
     4321 cgaggctgat acacaagcag ccgatggtga agttgcgcga cttaaacaac gacagatcga
     4381 ctatcgggtt atcgtcggtc agctcccaga caatcaggaa gcagatagcc accaccgcca
     4441 ccacggtaag gatgataatt tcctgtgatg aaaaccagtc cagctcttta ccgcggtcga
     4501 gcataatctg caggctgccg ataccaataa ccagcagtgc cagccccacg gcatcaatcc
     4561 gccgccgttt ggtgcgggtt tcacgtccgc gcagagtttg cagtgtcatc aacaccaccg
     4621 ccacgccaat cggcacgttg atgaagaata tccagcccca gtggtaatta tcgctgatat
     4681 aaccgccgag gatcgggccg caaattggcg cgacaatcac cgtcatcgac cacaacgcca
     4741 gcgcgatcga gcgtttggct ggcgggtagt tattcagcaa tagactttgc gaaagcggga
     4801 tcaacggccc ggcgacaatc ccctgaatca cgcggaagaa gatcagcata ttcaggctgc
     4861 tggagacacc acacgcccac gacgcaatag caaaggcgat ggtggaccaa aggaacagtt
     4921 tcacttcccc gacgcgcttt gccagccagc cggtaagcgg gatcgagatg gcattcgcca
     4981 ccccgaaaga agtgattacc cacgttccct ggctgagcga tgagcccaga ttcccggcga
     5041 tagtggggat cgccacgtta gcaatggtgg agtccagcac ctgcatgaat gtcgccagtg
     5101 acagcgcaat cgtcataatg accagttgcg cgccttccag cggtttttgc tgttgcatca
     5161 cacgcacctc tggattagcc agcgttagct tttacgatat cgtcgatcag tttattgaca
     5221 ggtgccaggc tgatttcacg cgcggtactt accgcgaccg gtgttgaacg tactttattt
     5281 gccagtacct gaccgtcacg gttagtggta ttgacgctca ccagtgtgga caaaccaatg
     5341 cgcagcgggt attgctccag ctgtttctgg tccagttcga tacgcacagg tagacgctga
     5401 acgactttga tccagttacc ggtcgcattt tgcgccggaa gcagtgagaa tgcgctacct
     5461 gtgcccatat ccagaccaac cactttaccg gtgtatttca catcatcgcc gtaaatatcc
     5521 gtggtgatag tgaccggctg accgatacgc atattggcaa tctgcgtctc tttaaagttg
     5581 gcatccaccc acatattggt ggctggaacg accgccatca gcggcgtcgt tgggctaatt
     5641 tgcgccccag gctgtaccgc acggcgggag acataaccgg tcatcggact gacaatacgg
     5701 gtacgctcca gcgccagcca ggcgttacgt acttcggtgg cagcctgttg cacggcaggc
     5761 tgatcttcca gtttagtccc cagaatcatc gcctgattgg cattgtattg ttgaatagcg
     5821 acgtccagtt gcgcctgggc actggtgacg gcgtcgcggg cgtgttgcag ctcttcgcga
     5881 ccaatcaggt tggcattgcc cagcggcaca cggcggttgt agtcgctttg tgcttttgcg
     5941 agggcgattt tctgcacctc aatattcgcc tgcaactgtt tgctgttaat catcagctgg
     6001 tgggtttggc gaacgctgga agccagtgca gttttggctt tttcaaacgc ctggcgagca
     6061 tctgtcgggt cgagagtgac cagcacgtcg ccttctttta caaaatcggt gttatcggcc
     6121 cagactttcg tcacgctgcc agacacctga gacataattt gaatctgatt ccctgccacg
     6181 tatgcgtcat cggtttcttc gaaatgacgc agtaccaaaa accaataaat ccctatcgct
     6241 acggcaataa ttataaagag caaggtgaga aggaggagca gacgcttacg tttgccgctc
     6301 ttctttaccg gttgctgcgg ggtttgagtc tccgcatttg cgctcatatt gttctccacg
     6361 atcttcttaa ctcatcggct gagccgacct gcttgccaaa aaggccagca tgttgcgatg
     6421 ctggccggtc attttttctt ttataaatct ggatttttga gcgagatgac gcgttagctc
     6481 atcgcttcga gaaccacacc gtcttgttcc atctggtcga gacgggagag caatttgcgg
     6541 gtgatttgct cgagctgatc tttttctgtt gtgctgagcg cggaccagag ttgatgcagg
     6601 cagttatgct gcggtggtaa aacctcgcgc aaaaactcgt gacctttttc cgttaattgc
     6661 agatgcaggc agcggcgatc gttatcgctt tcacgacgtt cgatccaacc gcgtttttcc
     6721 agttcatcgg caatacgcgt cgcgttggta cgggatgatc caagagcaca acttaattca
     6781 gaaggctgaa tactgtggtt ttcctgagac tccagcgtaa tcaacgccat aaacaacgtc
     6841 tcgttaatcc cctgagcctt cagcatttta ttgcggttct ccagcagctt gctttgcatg
     6901 tgcatgcaaa gacgagtcag aaggatctcc tgataaggaa aatcttcgtg gcggctggcg
     6961 cgaaatttta gcatttgttc aatgggcgta aacgaactat ccatttgggt atgacctcat
     7021 taatttcagc cgatatagta acgacagtga caaataaagt aaatgtattg ttttagaaaa
     7081 atgattcttg tgggttatat aatcgccatc actttccagg cgagcccata ggccagcgca
     7141 ctaagcagtg ttgggatgat aatgctgcgt gttttataga aactggcacc cagtaccgcg
     7201 aagccgacca gcgtgggcac gaaacggcgt gtatcgtgca tcacttctgg tgcggtagag
     7261 acaaccagca gagcgcatat cgaggcgatg ccaatggtgt cgagcaaaat acctaccgcg
     7321 ccacgtttgg ttgggcgggc attacccaca cgcaggcgca gcggcaaata gcggaagcaa
     7381 taattcgccg cgccaaccag taacccaggc agcagaacct catagctcat cgggcgctcc
     7441 ttgccagaat gcttggatta acgcagtgag gcagccacag acaatgcctg ccagaatggc
     7501 gacgggaata gaaaatagcg ttacgcctgc aagggcacca actaacgctg cggtaacgca
     7561 aagagattgt ttgcgctgga aagaggcgag caggaaactc ataaagagtg ccggaagcat
     7621 aaaacctaat gctgcttcaa cggcgggata accttgcagc aagccgctgc cagagaatgc
     7681 ccctattacc gtaccaaata cccacgatga ccatgaactg aaggcaatgc cgatcatcca
     7741 gttctcgctc cagcggcgat tattgcgtac cagttttgct gttgcggcgg caaaaacctc
     7801 atccgtcagg ccaaacgccc acagggcggt tttcgatttt tgcagacgct gaataatacg
     7861 gctacgcagt gacgggccat acaacacatg gcgaacatcc attgccatga cggtcagtgc
     7921 agcaacccac aaactactcc cggctgccag catcgcggta atgacgaact ggctcgcgcc
     7981 tgcataaatg atgcaggaga aaaaaacgct ttcgagagga gagaatccca gacgggtcgc
     8041 attcagaccg aacgcaaagg ccaccggaat ataactaata acaatcggta aactgtcttt
     8101 gcatccttcc atgaaggtcg ccgaaccagg agcaggctgt ggagtagggc tttccataga
     8161 gtgtacgctt aacgattgtt aatattataa atagactgaa tgaatatctt aaccttatca
     8221 gactgatggg cttcttaaca cccttataag tgtaaagcca cgaaaacggt tgctgattgc
     8281 ttaatttgcg gcttcctgac gatgaaaacc tcgccaccag accagtaagt taactagggc
     8341 aatcgtcgcg ccagccagac aaacgccagc ccaaccgcca tgttgccagg ctgaggctga
     8401 aattagcgaa ccggcggcac cgccaataaa gtagctggtc atgtaacctg cggtcaggcg
     8461 attacgcgca tcagggtgta ttcgataaat taccgtctgg ttagtgatat gcacgccctg
     8521 tacggtgaga tccagcacca ggatgccgat aatcagcgcc agtacggaag tgtgaccaaa
     8581 ccagatcgcc agccatgaaa gtaatagcag cagcagaccg aaagttgtgg tgtggtgcga
     8641 tttaccctta tcggcaaaac cgcccgccgg acgagcgccc aacgctccgg cagctcccgc
     8701 aagtccaaac agaccaatga caccatcgct gtagttaaaa ggtggagcgg caagcaaaaa
     8761 ggccattgag gtccagagaa tgctgaagtt ggcaaaggtc aggcagccca gcaatgcgcg
     8821 ggtgcggagg atcttatcgc tgataaacat gctgaaaacg gaacctaaca actgtgggta
     8881 gttgaggtgg gtttctgatt tcatttgtgg cagaccacgc cataatgcta gcgccatcag
     8941 tgccattaac accgaggcaa cccaaaagac ggtgcgccag ccgccgaggt ttgccagtaa
     9001 cccggcaacg gttcgtgcca gcaagatccc caacagcaga ccgctcataa tggtgccaac
     9061 cactttgccg cgtttatccg gtgaagccag cgtcgctgcc agcggaacca gaatttgtgc
     9121 cacgactgag aataaaccgg ttaatgccgt accgaggatc atcatcgcca gcgactgact
     9181 gctggcagtg attaacatgc cgcctgccgc cagtaaggtc atcgagacaa tcaggcggcg
     9241 gcgttcaaac atatcaccga ggggaacaag aaacagcaga cctgctgcat agcccaactg
     9301 cgcggcggta acaataaagc ctgccgaact ggcggaaagg gaaaagttac gcgcgatggt
     9361 gtcgagcaat ggctgggcgt aatagttact ggctaccgcc tgaccggtgg cgatagacat
     9421 cagcacgatc agcgccgggc taagctcatg attaggttta gtcattattt ttgttcatca
     9481 ggtgatgatt aagctggaaa tcaataatag cggatgttga tgaatggggg cgaaagatag
     9541 cttgttgaaa tgtgcgatgt ggtttgtagg catgataaga cgcgccagcg tcgcatcagg
     9601 cattgtgcac agaacggcgg atgcggcgaa aacgccttat ccgccatacg gaatgagtgt
     9661 attttgtagg catgataaga cgcgccagcg tcgcatcagg cattgtgcac agaatggcgg
     9721 atgaggcaaa aacgacttat ccgcccgaat aaaaattact tctgcgctgc cagcgcctca
     9781 ttcacccagc catcgaactg ctgctggtgg gctttgatcc aaccatcaac atggccctga
     9841 atatcgcctt ctgaggcttt gccgtcatgc ataatggcgt tctgggcgtt aatatctgcc
     9901 actggcaact gcataatggc aaaaagtttc gctgctgccg ggtttttctc ggcccaggct
     9961 ttgttggcaa cgatatgcat ggtgctgacc gggaagccat aattcgcacc attcggcagt
    10021 ttggtatcgg cgtttttatc gcccggcagt gcggagaacg gcacctgcaa ccagaccaca
    10081 tctttccctg gcttcagctc attactcacc cagtacggcg tccaggtgta ataaaacacc
    10141 ggtttgccct ctttgtagcg actgatggtg tcggccatca tcgctgcgta gttcccctga
    10201 ttatgcgtca cggtgttggt cagttcatac gcggcaagct ggtggttgat cgcaccttcg
    10261 cagccccagc ctgggttaca gccggttaaa tccgctttac cgtcgccgtt ggtatcgaac
    10321 agtttggcga tcttcggatc tttcagttgt gcgatgttgg tgattttgta ctggtcggcg
    10381 gttttcttat cgatcaggta accctgtgcc gcgccgttaa cgaatatccc ttcacaataa
    10441 aatttcttat cgccaccggc agcttcgtac atgttgtcat gcagcggcgt ccagttcacg
    10501 gcggtgaagg ttgcatcgcc ggaagcaagt gaggtgtagc caacgttgta atccacttcg
    10561 ctgggtttat tgacggtata acctaatttc tccagtgcac gactgaccag cagcgtctgg
    10621 aaggtttctt cagtgatggt gctctgaact ggattaacag taatgccttt gcccggcaga
    10681 tcggctgcaa aagtttgtgt agagataagc gtggcaaacg ctgtcgcaaa aagtacgcta
    10741 tgtcgcattg ttattccttt ttttggcagg gtgataatgc ccggcgttac cgggcaagtg
    10801 cagagttact taatgaatgg gcgggtcagc agaccgacag ggccagtggt gtaccagcga
    10861 cggttgccgc gactgcgtga gtcgcgccca acggcctgcg tcaggcggtc gaggataatg
    10921 gcgaggatca caatcccgac gccgccaacg gtggcaaggc ccatatccag acgaccgata
    10981 ccgcgaagta ccatctgacc cagcccgccg acggcaatca tcgaggcgat gaccaccata
    11041 gaaagagcca gcatcagcgt ctggttaacg cccgccataa tggtcggcat cgccagcggt
    11101 aactgaactt tgaacagcat ctggcgcggg ctggcaccga atgagcgcga ggcttcaatc
    11161 agatccgccg gaacctggtt aatccccaga atcgtcagac ggataatcgg cggcagagca
    11221 aagatgatcg tcaccactac gcccggcacg ttaccgatac caaacagcat gacgattggt
    11281 accagataaa caaacgctgg cgtggtctgc atggcatcaa gcagtggacg aataattttc
    11341 gccgctcgcg gacttctcgc cagccatatc cccaacggca aaccgatgac gatacagaac
    11401 agcagggcgg ttaacaccag cgccagagtc accattgcct gcgaccaggc accgattgcg
    11461 ccgatggcaa tcagcgaaac cagcgtcgcc acgcccattc cgaccccgga aatctgccag
    11521 gcgatgagag cgaaaacgat aatcgccact ggcgctggca tacccagcag caattgctgg
    11581 aaactgttga ggatataatc aaccggaacg cgcacgccct ggaagacggg acggaaatgg
    11641 gtaacaaccc agtcgatccc ttcagtgacc caactgtcga gcgggatcag cgttttatgg
    11701 aacggatcga gaatattaaa atgctcgacg tttggcgcag gcgtactggt cagccagtca
    11761 gcaccaccgc cgtcagtcgg tgcagtcgtc ggtgtacccc aggcgtctgc ggattgcgcg
    11821 gcactgtccg ccgctggcgt ggtatcccac ggattatttt gatcagccat tatttacccc
    11881 ctcacgatct aaagcgcgca gcagcattcc tttcgaaatg atgccgacat actgttggtc
    11941 ctcgtcgacc acgggcaccg cacagggtgc ctgtccgaca tgagagagca actcgctaag
    12001 aggcgtttgt gcatcgactg ctaacggcgc atcaatcagc gccgcatcaa gaccttgctg
    12061 ctgcgttaac gcggttttaa gcgaatcgat ggagactgcg ccgacaaact tattaccgcg
    12121 ttcgataacg tagccatatt cgcgatcttc atcctgcaat aatttcagtg ccgaacgtgg
    12181 gccgaagcca ggggttttac gaattaagcc attcggtgtc cggcgggcaa tatctttcgc
    12241 actgaatacc tgactaatat caacgccacg gaagaaggta cggacataat cattcgccgg
    12301 attattgaga atttcatccg gtgtgccgac ctgtaccact tcaccatttt gcataatggc
    12361 aattcggtcg ccaatacgca tggcttcatc gagatcgtgg gaaataaaga caatggtgcg
    12421 ctgatgtttc gcctgtaatt ttaccagctc atcctgcatc tcggtgcgaa ttaatggatc
    12481 gagcgccgag aaggcttcgt ccattaataa aatatccgga ttaatcgcta acgcgcgggc
    12541 taatcccaca cgttgacgca tcccgccaga gagttcatcc gggtagctgt gggcataatt
    12601 ttccagcccg acctgacgca gtgcatcaag ggctttttcc cggcgttctt cggcattaat
    12661 tccggccaat tccataccga acgcagtatt gtccagcacg gtcatatgcg gcattaaggc
    12721 aaaggactgg aagaccatcg caatcttttt tctgcgcacc tcacggagtt tggcatcaga
    12781 tattttggca atatccacac catcaatcag cacttgcccg cgggtgggtt caatcaggcg
    12841 attgagaagg cgtaccattg tggatttacc cgagccggat aatcccatga tgacaaatat
    12901 ctcgccttct tcaatggcca gactggcgtc ttttacgcca agcgatagcc cagttttttc
    12961 cagaatttgt tcttttgaaa gtccttgttc gatatatttg aatgctcgct gtggatgctc
    13021 accaaatatt ttataaaggt ttttaatttc taatttaatt gccatgcaat agaaagattc
    13081 ctgttatttg tttatgtcga tatattaccc aatggaaata gttacttttt tctaccctaa
    13141 catactgaga atctgaggca acccctgatg gcaaatgtgg catgaatatt gatgcgagca
    13201 aatcgccgga atttcccgtg atataagggc tgagagcaaa tcgagaaaag ttgatatttt
    13261 ttgtcaaaga tagcgaaaaa acgcctgaat attgtggttt caggcgaata atgcaacaga
    13321 gtatcagtgt agatcaccac aaaatatttg cgtgataaaa agctatttgg cgggaattat
    13381 ttccctgtcg cgggtagtga tatttttgaa aataacaccc tcagaaattc cagtcttcat
    13441 cttctgtttc aaccgctttc cccatcacat aagaggagcc tgaaccggaa aaaaaatcgt
    13501 gattttcatc ggcattcggc gaaagcgcgg cgaggattgc cggattcact tccgccattt
    13561 ctgcgggaaa taaaggttcg tagcccagat tcatcaaagc cttattggcg ttgtaacaga
    13621 gaaacgcttt cacatcgtca gcccacgggg tttcggcgta cagctcatcg gtgtattgca
    13681 gctcgttgtc gtagagttcc agcaacaaat cgaaggcgaa actcttcaac tcttcacgtt
    13741 gtcccagaga tatcttttcc atgtttttct gatatttata gcctatgtag taaccgtgga
    13801 ctgcttcatc acggataatc agacggatca ggtctgcggt attggtcagc tttccgcggc
    13861 tggaaaaata catcggcagc cagaaaccgg aatagaacaa aaaagattca agaaacacac
    13921 tggcgatttt ctttttcagc ggatcatcac cgcgataatg ttgctgaata atctgagctt
    13981 ttcgctgcaa cggtgcgttt tcttcactcc aggcgtaggc ggcatcgaca tctttggtct
    14041 ggcacagcgt cgagaaaatc gaactgtaag agcgggcatg aaccgcttcc ataaagctga
    14101 tattcgataa taccgcttct tcatgaggcg tgagtgcatc gggcatcagc gaaggcgcgc
    14161 cgataacatt ttgcagcgtg tcgagcagcg tcaggccagt aaaaacgcgc atcgtcagtt
    14221 gttgttctac gacagttaat gtctgccagg caggaatatc gttcgacagc ggcacctttt
    14281 ctggtagcca gaaattgctg gtcaggcgat tccacacctc cagatcttta tcgtcagata
    14341 tcttgttcca gttgatggcg ctgatacgtg agagtttcat agatattcct taaagtgcac
    14401 aggagacgca gccttcaatt tcagtgcctt ccagcgccat ctgacgcagg cgaatgtaat
    14461 agagcgtttt gatacctttg cgccaggcgt aaatctgcgc tttgttgata tcgcgagtgg
    14521 tggcggtatc ggggaaaaac agcgtcagcg acaaaccctg atcgacatgg cgagtcgctt
    14581 ccgcgtaggt gtcgatgatc ttttctgcgc cgatttcgta agcatcctga tacagcgcca
    14641 gattttcgtt agtcataaac ggggcagggt agtaaacgcg tcctgttttg ccctctttgc
    14701 gtatctctac tttcgccaca atcggatgaa tactcgacgt agcatggttg atataagaga
    14761 tagaaccggt tggcggcacc gcctgaagat tctggttgta aatgccgtag cgcatcacgt
    14821 cgtcgcgcag ttgcgcccac atctcacggg taggtaacgt aataccgctg cgggcaaaca
    14881 gttcgccaac tttcgccgtt ttcggctgcc agttaccttg cagatattgg ctaaaatatt
    14941 caccactggc ataacgtgac tgtttgaacc cggcgaaggt ttcaccgcgt tcgcgcgcca
    15001 gcaacatcga ggtacgcagt gcgtgccagg tgatggcata gaaatagaga ttggtgaaat
    15061 ccagtgcttc cggcgaacca taagcgatgc cttctcgcgc cagatagccg tgtaaattca
    15121 tctgccccag tccgatggcg tgtgaggcgg catttccggc ttcgatggac ggcacgctgc
    15181 ggatatgact catatctgac accgccgtta aaccgcgcac ggcagtctct accgtgcggg
    15241 caaaatcggg ggaatccatg gtgtgcgcaa tattcaacga acctaaattg caggagatat
    15301 catggcctgt gcgggcatag tcgagattct cgtcatactc tgaggcacag ttgacctgca
    15361 aaatctctga gcagagatta ctcatattta tgcgcccggc gacagggtta gcacggttta
    15421 ccgtgtcttc atacatgatg tagggatagc cggactcaaa ctggatttct gccagccgct
    15481 ggaagaaatc acgggcgttg aggtattttt tgcggatgcg ttcatcggca accagttcgt
    15541 catagtgttc gctgatggcg atatcggcaa acggcttgcc ataaactcgc tcaacgtcat
    15601 agggcgaaaa cagcgccatc tgcgcattct cttttgccag atggaaagtg atatccggga
    15661 tcaccacgcc cagcgacagt gttttaatgc ggattttttc gtcggcattt tcccgttttg
    15721 tatcgagaaa acgcagaata tcgggatgat gagcatgtaa atagactgca cctgccccct
    15781 gacgcgcgcc gagttggttg gcataggaaa atgcgtcttc cagcattttc atcaccggaa
    15841 ttacgccaga agattgattt tcaatacgtt taattggcgc gcccgcttct cgcagattcg
    15901 acagcaaaaa tgccacgccg ccgccgcgtt tcgacagttg cagtgcggaa tttaccgccc
    15961 ggccaatcga ctccatattg tcttcaatac gcagcagaaa acaggaaacc agttcgccgc
    16021 gctgctgctt accgcagttg aggaatgtcg gcgtggctgg ctgaaagcgt cctgacagca
    16081 tttcatcggt cagttgcaac gccagcgtcg catcgccttg tgccagcgtc agcgccacca
    16141 tcgtgactcg atcggcaaaa tcttccagat aacgtttacc gtcgaatgtc ttcaacgtat
    16201 agctggtgta aaacttccat gccccgagga atgtctggaa acgaaaaccg ctggtgtgtg
    16261 cgtgggcaaa cagcgtaatg acaaagtcgc gagagtagcg attaagaacg ctttcatcat
    16321 agtaaccttc gttgaccagc cagttcaggc gctgctgctg gctactgaag gtcacactgt
    16381 tcggacgcac atgcgtcgcc ataaaggcgt caacggcctg gcggtcttta tcgaactgaa
    16441 tgcgacctgc gctatcgtag aggttaagca tcgcattcag cgcgtggtaa tccatcgttt
    16501 cctgcgtcag gcattctgcg gttgtcgttg ccaaaattcg gttactcctt tacgaacgtt
    16561 ttcgatatcg ctttgcgtac ccatgagttc aaaacggtac agccacggca cgccgcattt
    16621 ccgggcaatc acatctccgg cgcggccata cgcctcacca aagttgcgat taccagaagc
    16681 aataacgccg cgaagcaacg cccggttgtg ctcgtcgttt aaaaagcgaa ttacctatcg
    16741 tggcaccgcg ccagccgtac cgccgccgcc gtaagagggc acgatcagga tgtaaggctc
    16801 gtctacctga atccgttccc gctcattgag cgggatgcgc accgcgggca gacctaaacg
    16861 ttcgataaaa cgctgcgtgt tttcggagct gctggagaag tagacgagct ggctcatgca
    16921 ctggccgcgt gtggcgctgg atgcagacgg ttaatcatgt ccggacggaa accagaccag
    16981 ctaagatcgc cagcaatcac taccggcaac tgacgaaagc cctgagcacg caacgcttcc
    17041 gctgcttcag gaacgcgatc gacattaatc atttcaaaat caaagccccg gttttccatc
    17101 gcccgtttgg tggcgtggca ctgaacgcaa tcgttacgag tgtaaatagt tatgcgcatg
    17161 attcgtattt ccgtttaaaa tgaagatacg gcgcgatgat acgcgtcggg ttgtctctct
    17221 gttgatacag agatactaga tgtagttgaa aaaagattca accacacaat atatagcaaa
    17281 tagtgatcga aattacccta gatatgagcg tgacggggga gggggatttt tgtgattcac
    17341 caggcaaaaa gaaaccccga agacaggctt cggggttaaa gacgcgtatt tattatcatt
    17401 tttgcactac gatttgcgca tgcttaacag tgcgccgata aaaataccta ccgcagctgc
    17461 tgtacccacg ctacaccagg gtctttcacg aacaaaagaa tccgcgcagc caacggcatc
    17521 gcgtgcggct tgctggacgc gagtacgacc atgcattcgc gccctggttt ctttcagcaa
    17581 tgcctgagct ttactgcgtg cggcttcagc ttcccctttg gcgtcgctgc cccaggactt
    17641 caatacagat tcgaggctgt cagctaattg attgacatca ttctggatat cctgcacgcc
    17701 atcatcaacg tcgttgcggt tcggtcggtt aaacatatga tcccccatta gaattcgatg
    17761 taattttcag tgtagaccat aattttctta actgaggttc atcgcggctt tatggacttt
    17821 tctgaaagcg ttgtgaatgc gcaatcaggt aaggtaggcc cgcggttgat aataacgagg
    17881 atcaaatatg tatttacgac cagacgaggt ggcgcgcgta cttgaaaaag tcggttttac
    17941 tgtcgatgtg gtaacccaga aagcttatgg ttaccgccgt ggggaaaatt atgtctatgt
    18001 taatcgcgaa gcgcgaatgg gacgtaccgc actggtaatt catccaacat taaaagaacg
    18061 tagttcgaca ctggctgaac ccgcttccga tattaaaact tgcgatcatt atcaacaatt
    18121 tccgctctat ttagcaggcg agcgacacga gcattacggt atcccgcatg gcttcagttc
    18181 gcgtgttgcg cttgaacgtt atttgaatgg tttatttggc gaagccagtt aaagacgcga
    18241 ctggcgatgc cagtcgcgaa aagaagagtc aggcttttac ctgctggtaa tggctgactt
    18301 taaacagtcg gcggcaataa tcgaggaagt agccataaac cgcccccatc aacatcgaaa
    18361 caacgatgtt tgaactgacc gccgccataa tctgatgcca gtctgcgccc accactaaca
    18421 agatcgccac atacaccggt gactggaacg tcacataagc caggatatca gccagatttt
    18481 ttatccagcc cgacgggcta actttgcgtg ccgcgcgcat aaatagatcg cggtacatac
    18541 cgtatggcca tgcaattaag atgttcaccg gaatcgctac caatctggaa taaaaagact
    18601 gttcgaagct cattccggag aggaaaactt caatacacat gttcacgaca gaacagtaaa
    18661 caaccatcgc gaacgtatct gcaactgcat gacgcaagcg tgactgcggt gagaacatgc
    18721 taatgctcct taataaaagc gtaatgggat gttaatggag atgaaagcag tggatctcat
    18781 tgccatctgc attttcgcat agcgtatatc gctggctatg actattcaat tagtgaaaaa
    18841 tttttattta gtcagttttt gtagataaaa tattccgtaa cggctcgttt tttgtacatt
    18901 gtggattttt acatgctgat atgatttatt ttcaattaaa tcaattagat atgaccttgc
    18961 ctttaatttc actaatgaaa gtttgggggt gaggtaacgc tataagcgta gagaattcgg
    19021 tgtatgtcgt gaccgtgtca tttacagcat ttttgatgcc agaaaatgta cattaacagc
    19081 agaaagcacc agtg
//
LOCUS       DAJVQG010000076        19010 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJVQG010000076 DAJVQG010000000
VERSION     DAJVQG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19010
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.076"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..298
                     /gene="csgD"
                     /locus_tag="OQM83_002367"
     CDS             <1..298
                     /gene="csgD"
                     /locus_tag="OQM83_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCS1520614.1"
                     /translation="NGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHREKE
                     ILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            303..692
                     /gene="csgE"
                     /locus_tag="OQM83_002368"
     CDS             303..692
                     /gene="csgE"
                     /locus_tag="OQM83_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCS1520615.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            717..1133
                     /gene="csgF"
                     /locus_tag="OQM83_002369"
     CDS             717..1133
                     /gene="csgF"
                     /locus_tag="OQM83_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCS1520616.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1160..1993
                     /gene="csgG"
                     /locus_tag="OQM83_002370"
     CDS             1160..1993
                     /gene="csgG"
                     /locus_tag="OQM83_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HCS1520617.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2058..2549)
                     /locus_tag="OQM83_002371"
     CDS             complement(2058..2549)
                     /locus_tag="OQM83_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCS1520618.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2651..3205)
                     /gene="ycdY"
                     /locus_tag="OQM83_002372"
     CDS             complement(2651..3205)
                     /gene="ycdY"
                     /locus_tag="OQM83_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCS1520619.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3229..3966)
                     /gene="ycdX"
                     /locus_tag="OQM83_002373"
     CDS             complement(3229..3966)
                     /gene="ycdX"
                     /locus_tag="OQM83_002373"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCS1520620.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4021..4959)
                     /gene="ghrA"
                     /locus_tag="OQM83_002374"
     CDS             complement(4021..4959)
                     /gene="ghrA"
                     /locus_tag="OQM83_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HCS1520621.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5193..5280
                     /locus_tag="OQM83_002375"
     tRNA            5193..5280
                     /locus_tag="OQM83_002375"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5227..5229,aa:Ser,seq:gga)
     gene            5325..5461
                     /locus_tag="OQM83_002376"
     ncRNA           5325..5461
                     /ncRNA_class="other"
                     /locus_tag="OQM83_002376"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5552..6238)
                     /locus_tag="OQM83_002377"
     CDS             complement(5552..6238)
                     /locus_tag="OQM83_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCS1520622.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHEWADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(6819..7607)
                     /gene="phoH"
                     /locus_tag="OQM83_002378"
     CDS             complement(6819..7607)
                     /gene="phoH"
                     /locus_tag="OQM83_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCS1520623.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(8228..9499)
                     /gene="efeB"
                     /locus_tag="OQM83_002379"
     CDS             complement(8228..9499)
                     /gene="efeB"
                     /locus_tag="OQM83_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCS1520624.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(9505..10632)
                     /gene="efeO"
                     /locus_tag="OQM83_002380"
     CDS             complement(9505..10632)
                     /gene="efeO"
                     /locus_tag="OQM83_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCS1520625.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10690..11520)
                     /locus_tag="OQM83_002381"
     CDS             complement(10690..11520)
                     /locus_tag="OQM83_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCS1520626.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(12186..13694)
                     /gene="putP"
                     /locus_tag="OQM83_002382"
     CDS             complement(12186..13694)
                     /gene="putP"
                     /locus_tag="OQM83_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCS1520627.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            14116..18078
                     /gene="putA"
                     /locus_tag="OQM83_002383"
     CDS             14116..18078
                     /gene="putA"
                     /locus_tag="OQM83_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HCS1520628.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(18118..18756)
                     /gene="rutR"
                     /locus_tag="OQM83_002384"
     CDS             complement(18118..18756)
                     /gene="rutR"
                     /locus_tag="OQM83_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCS1520629.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
BASE COUNT         4833 a         5077 c         4625 g         4475 t
ORIGIN      
        1 caatgggttg caaggcgtcc tgcgcggcga atgctacttt acgcaaaagc ttgccagcta
       61 cttgattacg cattcaggta actatcgtta taacagcacg gaatcagccc tccttactca
      121 tcgggaaaaa gagatcctga ataaactgcg tatcggcgcg tctaataacg agatcgctcg
      181 ttcgttgttc atcagcgaaa atacggttaa aacgcatctt tataatcttt tcaagaagat
      241 agccgtaaaa aaccggacac aagcggtttc ctgggcaaac gataacctca ggcgataaag
      301 ccatgaaacg ttatttacgc tggattgtgg cggcagaatt tctgttcgcc gcagggaatc
      361 ttcacgccgt tgaggtagaa gtcccgggat tgctaactga ccatactgtt tcatctattg
      421 gccatgattt ttaccgagcc tttagtgata aatgggaaag tgactatacg ggtaacttaa
      481 cgattaatga aaggcccagt gcacgatggg gaagctggat cactataacg gtcaatcagg
      541 acgttatttt ccagactttt ttatttccgt tgaaaagaga cttcgagaaa actgtcgtct
      601 ttgcactgat tcaaactgaa gaagcactaa atcgtcgcca gataaatcag gcgttattaa
      661 gtacgggcga tttggcgcat gatgaattct aaataaaaaa ttgttcggag gctgcaatgc
      721 gtgtcaaaca tgcagtagtt ctactcatgc ttatttcgcc attaagttgg gctggaacca
      781 tgactttcca gttccgtaat ccaaactttg gtggtaaccc aaataatggc gcttttttat
      841 taaatagcgc tcaggcccaa aactcttata aagatccgag ctataacgat gactttggta
      901 ttgaaacacc ctcagcgtta gataacttta ctcaggccat ccagtcacaa attttaggtg
      961 ggctactgtc gaatattaat accggtaaac cgggccgcat ggtgaccaac gattatattg
     1021 ttgatattgc taaccgcgat ggtcaattgc agttgaacgt gacagatcgt aaaaccggac
     1081 aaacctcgac catccaggtt tcgggtttac aaaataactc aaccgatttt taagccccag
     1141 cttcataagg aaaataatca tgcagcgctt atttcttttg gttgccgtca tgttactgag
     1201 cggatgctta accgccccgc ctaaagaagc cgccagaccg acattaatgc ctcgagctca
     1261 gagctacaaa gatttgaccc atctgccagc gccgacgggt aagatctttg tttcggtata
     1321 caacattcag gacgaaaccg ggcaatttaa accctacccg gcaagtaact tctccactgc
     1381 tgttccgcaa agcgccacgg caatgctggt cacggcattg aaagattctc gctggtttat
     1441 accgctggag cgtcagagtt tacaaaacct gcttaacgag cgcaagatta ttcgtgcggc
     1501 acaagaaaac ggcacagttg ccattaataa ccgaatcccg ctgcaatctt taacggcggc
     1561 aaatatcatg gttgaaggtt cgattatcgg ttatgaaagc aacgttaaat cgggcggtgt
     1621 tggggcaaga tattttggga tcggtgcaga cacgcaatac cagctcgatc agattgccgt
     1681 gaacctgcgt gtcgtcaatg tgagtaccgg tgagatcctc tcctcggtga acaccagtaa
     1741 gacgatactt tcctatgaag ttcaggccgg ggttttccgc tttattgact accagcgctt
     1801 gctggaaggg gaagtgggtt acacctcaaa cgaacccgtt atgctgtgtc tgatgtcggc
     1861 tatcgaaaca ggggtcattt tcctgattaa tgatggtatc gaccgtggtc tgtgggattt
     1921 gcagaacaaa gcagaacggc agaatgacat tctggttaaa taccgccata tgtcggttcc
     1981 accagaatcc tgacagataa aaatgccgga attaataatc cggcatttta ctgtttaagc
     2041 gaaagcgtga gtgcccctca cgctttattt ttgatttcct gctcacggtg agccgtcttt
     2101 gcactacgcg ccgccagcca caggccgctg tttttcattg cgtaaccaaa taccagccca
     2161 agcgccagcg aaggtaacac cagtttccag tcaccctgcc cggcaaacgt cgcgcatgcg
     2221 cctataaacg tccccggaac aaatgaaagc agcagctgtt tggcctgaat acacatcaga
     2281 aacgccacaa taccggttat gacataaccg agaatttcca gatgtggtgc cagtgcacta
     2341 ccgtaaataa tgaccatcgc ccacaccacg ccactaagca gggttgcagc ggagatcgcc
     2401 agccctttca gcccaccttg cgggcaggca aagtaggccg tacagcccaa gaagcccgcc
     2461 cagcttagta agcccaggga aacagccacc catccccaga taccggagag aatgcctgtt
     2521 gtgattgcaa tagagagaag tatgttcatg ccgcgcatga tagcagaaaa cgcggccatg
     2581 aatgagatcg agcacacatt ttatgcaaca acaaattatc tgttgcagtt atgagtgacg
     2641 atgatcacat ttactcttca gaatcttctt ccagctcgtc ccacattgca ctaatggcat
     2701 cacgggttag cggtgccatg gtgcgccaga aaggcgtggt tgcatgggcc tccactttgc
     2761 cgaggaacgc accacaccag ggtaacagat actcactgaa cagtgtttcc agtgcttcgc
     2821 tctcatcttc cgttgactga tcttccagcc aggaagccgc aagcagcaat gtgccgatgt
     2881 gatcggctgg cgtatccgcc agtggcatcc ctcgttcgga aagaaaagcg cgcacttccg
     2941 cttccgttac gtcctcaacc catgcgctac gatatggcgg cactgcacat tcatcgccga
     3001 taaacaacgc attgtaatcg gcagagactt gcgccatatc acagcttttc tgtaaacgcg
     3061 tcagtaactc atcctgctcc agtggccagt tcgcagccag tttcccctca cgaatcaggg
     3121 taaacagcgg caccagtaaa ggatcttgcg gttggcggta atagagcgaa cccagcacac
     3181 gacagaggat agaaaactcg ttcattaata tatttccatt actagtaatt aaaggtctgc
     3241 aaattccgca atcggtgcca taccgcgaga ttcaaggaag ttcagtaagc ggcgcggaga
     3301 aacattcaaa atgcgctctg gcggaaaatc taccgcgtcg aggattttaa gacactcttc
     3361 aaattccccc atggtaaacg cggtgtgaga atccgagcct aatgccaccc aacctccagc
     3421 atcacgtacc gctgcggcta ccgcacgaca gttgtcttca ctgcccttac gtgagtgtaa
     3481 aaatgaagaa ttattgattt ccagcgccac ctgatgtttc gcggctgctt cagcaacggc
     3541 tttcacatct atttcatatt tgggatttcc gggatggctt attatatgca cattgccgct
     3601 ggcgatagtg gcgatcatcg cttgtgtatt ggtcgctttg tcatgtggtg caaaaaccgg
     3661 ctcatgaaaa ccggcaataa tcagatccag cgagtcaaac attttaccgc tgcaatcaat
     3721 ttcaccatca acatttttaa tgttagcttc gatgccgcgc aggatcccta ccccatcaac
     3781 cactcgcggc cagatacgca tgttaatgaa gtgccagtga tgcggagcat cttccatatc
     3841 cgggccatga tcggtgatcg caaaaagttt aatgcccttt tgtttggcct gggcaatgta
     3901 atcactcaat gtgctatatg catgtgtgct ggcaacggta tgcatatgaa ggtcgacggg
     3961 atacataact ctctcctgta ttcaattccc gccaaggata gcaggaatcc tgatgcttta
     4021 ttagtagccg cgtgcgcggt cgacttgccc gcagaccctc tccccttttt cgagctgggc
     4081 aatggtgcga gaaatgtact ccacagcttc agcgggacgg gtaatcgcgg cgacatgtgg
     4141 tgttatcgtc acgcgtggat gttgccagag cggactttca ggcggtaagg gttcacgatt
     4201 aaaaacatcc aacattgcgc ctttaacttt gccgctatcc agcgccgcga gcaggtcatc
     4261 ttccacaaca tgaacaccac gcgccaggtt gaggagatac gcgccatccg gtaatttttc
     4321 gagtaattgt tgattaataa tgccgacggt ttcaggggta ttcggtaaca aattaatcaa
     4381 tacccgacat tggctcagaa atgcagacag ttcttcccgt ccggcaaagc tttgcacgcc
     4441 aggccacgat ttacgggttc gactccagca acgcagcgga aagcgccagg tttgcagact
     4501 ctgagcaact ttactgccca gtacgcctgc gcccaaaatg ccgatggtaa aatcttcccg
     4561 atgatattca ggcagcggtt gccaatgcga actattttgc tggatgcgat aatcgtcaaa
     4621 acgtcgaaac caatgcagca cctgactgac agcatattcc tgcatttgct cgcccatacc
     4681 ggtatcttcc aggcgaaaaa gtggaacaga agggttcagc atttcagggt gtgcctgtag
     4741 cttgctcaaa atagaatcaa caccggcccc gagtgcgaac accgctttaa gatcgcgccc
     4801 tgccagcatt tcaacaggag gatgccagac taaagcataa tcagcagagt cattatctcc
     4861 gcttttccat gctctgactc ttgcctgagg aatagctttg cgcagtgcct caatccacca
     4921 ttgggtatcg aacgttgggt gataaaagat gatatccata ctgactcccg aaaagcgttg
     4981 tgcgaaattt attcgcactt atcgttatga tctacaaagg ccaccagcat aacaaatccg
     5041 tggtcggtgg caaaaaaagc agatttcgct tattaaaacc acacattgat tgaagtttga
     5101 ataaacgcgc gattttttca aaaagtttgt tgacctcagg tcatgatttc cctaaattag
     5161 cgcccgttcc agcaagacag gaacgacaat ttggtgaggt gtccgagtgg ctgaaggagc
     5221 acgcctggaa agtgtgtata cggcaacgta tcgggggttc gaatcccccc ctcaccgcca
     5281 tatttaaaga agagctcgta cgaaagtacg ggcttttttt tcgtatattg cacacaccgg
     5341 ggggatgaga agccccgacc ggggttcgac aactggcgac agccagttgg acagaccgtg
     5401 aacgcagtga gcgggctgcc cgcagggcga gcgaagcgag tcaatccccc cctcaccgcc
     5461 atctttaaag aagagctcgt acgaaggtac gggctttttt tcgtatattg cacacaccgg
     5521 ggggatgaga agccccgacc gggatactta gttaatgcac cggttcactc tttgtcgccg
     5581 cgactaatgg gcgaattttg tcacgagtct cccagaccgg aggaatttcc tccaatttag
     5641 tgacagaagc tcgtacaaaa ggcacccgtg caagagtcat aattgtatcc aaccaacggt
     5701 cacgaataat gcgatccgcc cattcatgac tcgggtcatc cagctcaata acacagacta
     5761 ttttattgtt cttctttgaa accagaacat aatccattgc catgccatta taaattgcaa
     5821 agaaacgctt tctttcttct tcgctaaact caggttgttc tggaaagttg atcaagcgcc
     5881 ccagagagac ctggcaatgg atatgacaat aatgtccaaa ccatttttcc agattatcga
     5941 aatacatttt ttcacaacgc gtgattaacc actcttttct ttccaataaa ataccttcgg
     6001 caaccattcc ctgaaataac tcatctcgct cttgccaacg agagtcacga taggttttct
     6061 gcggtatata gctccttgtc gacgccttag cggcagggga agttgcagat gaagggacgg
     6121 gttgcgattt agcgatgatt accgatgagg caacttttcg gagtgtcaat tttctggcaa
     6181 cagcaaaacc ggcgataaat gcaattgtca aacaagttac aaaagccata aagctcataa
     6241 ttaattttca cctatccatg ttgattatat ttcattacca atgtaactcg ctgcagcgtg
     6301 ttacatgcca acaatgcaac gatcaatata tagttagtat catgttttga ttattacaac
     6361 catccctatt tagccattgc ggcttgcctt ccacaaaaaa ataacaacac acaatattag
     6421 caaagaatta attaagatta accttacatt tataatatta tccctaaaga ataaatcaga
     6481 ggaatatatt cggtgttaaa cagtcagtca aagtatagcc cattgttgat taaacctcat
     6541 aagcaaaatt ggcaccagat ctcctgtaca ggttgaatgg ccgaaaaaag ataggcagat
     6601 gctcattttt ttggcttctg gaagaaaaac tctccgcgct ggcagtatta cactggtctg
     6661 atgctatgac gaaaaatgcc tggtaagagt atctaaacca gactccagtt cagcttatca
     6721 acacgctcaa agtcgccccc ctgaacgcta acctgaagtg aaaatctgtt gaaataaaac
     6781 aaagcccggt tcgcccgggc tctgcaccga taacacactt aactgtaggc atgcagcgta
     6841 cgttggcaaa gtgccgaacg tacgcagtcc tctttaccga accggacgat cccaaccatt
     6901 tcatcttctt cgaaacgttc cagcgcgtca cttaatccgg agcacacgcc gcgaggcaaa
     6961 tcgcattgcg tgatatcacc gttgacgata accgtcacgt tctccccgag gcgggttaaa
     7021 aacattttca tttgcgcggc agtcacattc tgcgcctcgt caagaatgac gactgcattt
     7081 tcaaaggtac gtccacgcat ataggcgaac ggcgcaattt ccaccttccc tatttccggt
     7141 cgcaggcagt actgcataaa ggaagcccct aagcgccgga ccagcacgtc gtagaccggg
     7201 cgaaaatagg gagcaaactt ttctgcgata tctccaggta agaagccaag atcttcatcg
     7261 gcttgcagaa ctggacgggt gacgataatc ctgtcgacat ccttatgtat cagggcctct
     7321 gccgcttttg ctgcgctgat ccaagttttt ccgcccccgg cttcgcccgt ggcgaatatc
     7381 agctgcttac tctcaatagc cttcagatag tgcaattgcg cttcatttcg cgcgaggatg
     7441 ggcgaagtat cgcgactgtc gcgggccata ccaatggctt ctacgccgcc catctgcaca
     7501 agcgaggtga ccgattcttc ttcacgctgc ttatggctgc gcgaatcccg tctcagcaca
     7561 cgttttgcct cgcgacgagc tttgatcact gctttttgtc ttcccataga gagcaccttg
     7621 agttgtttgt attcatcaca cgcgccgttg gcagcgcgat tatgcgcacg aacatcagag
     7681 ggttggcttc cttgtaagcc atagtttgct tttggataaa atgccgaaaa acggctacgc
     7741 gcaccgttta cggcgtcggt aacacatgaa aagaaaggat gaggttgaaa atgcaaagtg
     7801 acgagatgac taccggagga gaaaactccg cgagtggtgg cgcgttgatt atctaaaaca
     7861 tgtccagtac aggacgttac catccgcgat ctccatagtg actgactatc actgccggga
     7921 acttccgctg ctacttaata agtacaacag atctcgcatt tattgcaaca atatatttac
     7981 ttatatttaa ctataaaaca ctatttcagt gacattagtt tctactggaa agatgacaga
     8041 gtgatgacag tgatgaaaaa agctgtgtgc tttcagcagg atttgcatca ggcatcagtg
     8101 cacgatcgcc ggatgcggcg tgaacgcctt atccagccta ccaaacccgc actgattgta
     8161 ggcatgataa gacgcgcaag cgtcgcatca ggcatctgcg cacaaatgcc ttatccgcct
     8221 aaaaacatta aacccgcaat aacgcgcttc cgagataatc gttcgcgtcc ttcactcccg
     8281 gcagcgaaaa aaaataaccg ccgccgatag gtttaacgta ttcctccagc gcttcgccat
     8341 tgagcctttt ttgtactgtc aggaagcctt tttccagatc gtgttggtag cagacaaaca
     8401 gcaaccccat atccagttgt ccggagttgg tgacccccaa cgaataactg tagccgcgac
     8461 gcagcatcag gctggactca ctctccgccg tgcggggatt cgccagccgg atatggctgt
     8521 ccagcgcaat caccttccct tccgggtcgc tggcgtaatc aggcacatca tgctcatgct
     8581 gcattcccag cggcgcaccg gtttgcttat cacggccaaa aatcgtctgc tgttctttca
     8641 gcggcgttct gtcccaaaat tccactcgaa actgaatcaa gcgtactgcc tgatagctgc
     8701 caccgattgt ccacgcaggc tcctgctgat ctgccgttac ccacaccact ttttgcatca
     8761 acttatcatt ctggctatcg ggattggcag tgccgtcttt gaaacccagc aaattaatcg
     8821 gcgtctcttt gcctttacta cgcgacacgt gatcggaaat aaacccttcc cgcttccagc
     8881 gcacactgag caaatccggc gtgtgtttga tgatatcgcg cagcgcatgg ataaccgtgt
     8941 cctgggtgtt ggcgcaaatc tgtagcaaca catcaccatg acataacgcc gcatccagcg
     9001 agtcgttggg gaaacgcgtc atcttctgca gcttttttgg catctgtggc gcaaggccaa
     9061 agcgctcatc aaacaatgag tgacccaccg ataacgtgat ggtgagatta tcgggcgcaa
     9121 tgtagccgcc aagaatgccg gaatcgagtg gtggcaggcg cggatttggc gtttctggtg
     9181 ctgctccgcc ctgagtcaga aaagcaaaac gctgagtcaa caagcgaaac aaccgctcaa
     9241 gatcggcttt atcgctggca agcacatcaa acgccaccag catcattgcg gcctgttgtg
     9301 gcgtcaggat ccctgcctga tgctcaccat aaaacggctg tttctcattg cgtgcatccg
     9361 gtgaaagcgt gcccggtgca ctttgcgttt tttgtgcatg agcgaccgga caacttcccg
     9421 ccagcgccag cgcaccaatc cctttcagta aacgtcggcg tgacggttca ttcacgccgt
     9481 tttcatcttt atactgcata acgcttaatc cagtcccagc acaccgcgaa gttgcgccag
     9541 atcttccgcc agcgcagtaa tcggtccttt cagtgcattc cggtcggcat cggtcaattt
     9601 gtcgtaggtt tcaaaaccgt ctttagtacg gtatttcgcc agaatggtat cgaccttttt
     9661 aaagttggca tcgacttttg ccagcagttc cgggttggct ttttgcagtt gtggacgcag
     9721 caaatcgaca attttctgcg agccttcaac gttagcctgg aaatcccaca gatcggtgtg
     9781 gctgtagcga tcttcttcac cgctaatttt gctggctgcc acttcctcaa tcagtccggc
     9841 tgcgccgccg accacttttg aaggtgggaa agccagttca ctgatgcgtt tttgcaaatc
     9901 gaccacatct gtataaagct gctcagcgta ctgatccatc cctttggtgg tgttgtcgcc
     9961 aaacaatgct ttttccagac ggtggaaacc agtgaatttt gggtcggcgg ctttttgctc
    10021 gtaatcatct tcacgggcgt caatgctgcc atccagatcg gagaacagtt cagcaatcgg
    10081 ttcaatgcgc tcatagtgct ggcgcgtcgg cgcatacagt gctttcgctt tttcgatatc
    10141 gcctgcttta atcgcgtcgg taaaggcttt ggtgtcggtc accagctgcg tggtttccgc
    10201 catgacatac gctttatatg cagtaattgc accaccaaga cttaacagcg catcactttg
    10261 cgccgcatcc gccgttgcct cacctttgac gatcaacttc cctttcgggt tagtcagcag
    10321 accgcaggtc atatcgtatt cgccaggctg taaattcgcc gtcattttct ggctaaaacc
    10381 aggggcgata ttttcccgct cttccaccac catcacgcct ttgaggatct cccactccag
    10441 cgccttctgg ctatggttct gaataatgaa ctgtgttttc ccggcgttaa ccgtaatggt
    10501 catcggttcg cactgcttat ccgtcacggt cactttgacc tgcggcacat cagcggcgtt
    10561 agccataaaa gcagaagaaa acagcgcagc cacgctcaac tgcaatgcgt tacggcggaa
    10621 gttaatggtc atgacgagac tatcccttta aagtatgttg taactaagca agagtttgcg
    10681 tcgtatttgt tacgcggagc gagacgctgt cgcccctgcg cgtggtggca gagcaaatgc
    10741 caccagcgcc gggatgagat aaataaacca gacggcgact tcgctgacgc tcggtgcttc
    10801 ctgatagcca aaaatccctt ccatcagcgt gccaaacagc gagtgagttg agagcactgc
    10861 gctcatatcg aaggcgattt cctgaaagtg gttccacaat ccggcttcat gaaatgcgcg
    10921 aatggcacca gcagccagcc ctgcggcgac gaagagaata aacaggctgg tccatttaaa
    10981 aaatgcacca agattgaggc gaataccgcc ccagtagagc aggaagccta gcaccacggc
    11041 agtagcaaga ccgagcattg cacccagcgg cggccagatc ccgacatctt gttgaaatgc
    11101 cgccagcagg aaaaagaccg actccagccc ttcccttgca acggcaaaaa agaccatcat
    11161 caccagcgcc cagccatgat gatttccacg ctgcaatgcg ctatcgactg cctgctccag
    11221 ttgtactttg acgttgcgcg acactttgcg catccagaaa accatccagg taaggatcac
    11281 cacggcgatc accgccacga taccttcaaa cagttcctgt tctttttgcg gaaattcgcc
    11341 ggtggtttcg ttaatgaaga tacccaagcc caggcacaac gcagcggcaa gcaacacgcc
    11401 aatccacatc acgccaatcc atcggcctcg ctgggtacgc ttaagatagc tggcaatcaa
    11461 actgacaatc agcgcggctt caagtccttc gcgcaacata atgagaaacg gaacaaacat
    11521 gccacccacc cttaaacgtt tttctgagtc aatccatgca aagaaaagta aatcgcagtg
    11581 atagcgatta tcattacggc aaagaaaaat acaagcggaa tgtttataaa atgatgttgg
    11641 aatgtaaaca aagagaggta ataccagggg atataaacga ttattccagg tacaaagatg
    11701 ggattgttta caacgagcca tgtgttcttg ccagatgccg cgtaaacgcc ttatccagcc
    11761 gacaaaactg tccagactca atgcatcaca gcttccatgt aaaactgata agcgggtgta
    11821 tcaagcaatt tcgtccatcc gcgattatca aatctaactt tttgcccgga acataataac
    11881 agcgcgggtg ttggtggcgt caaaatcgct gaaacgttcc ccgaaggccg gggcagccca
    11941 catggatgtg ggctgagggc gcgtcttaca ggacgttacc tcgcgcccga cccggtagcc
    12001 gaaggggata agctgaaggt accgcgcagc ggcgattttg ttcgccagag cccgggggtg
    12061 cagggggcgg cggcgactgg ccgccccctg cgcgctccct gcaccattca atttatatgg
    12121 cgaggcacat taagggagcg caacccgctg ctgaagccaa acaaaaccgc cgcaggctaa
    12181 gtcccttagt tttcctgcaa ccgtgacggc ggagccgaat gatagtgcgc atcggcctcg
    12241 gcaaagcgtt tttgcatcgc cgctgacggc gctttaccca gcaaactaaa cactacaatc
    12301 ccgatactgc cgaagatgaa gcccggaata atttcgtaca gtcccagcca gccgaactgc
    12361 ttccagacga taactgtcag cgcaccgatg atcatccccg ccagcgcacc gttgcgcgtc
    12421 atgcgtgacc acatcaccga gaacagcacc accggaccaa acgccgcgcc aaagcctgcc
    12481 cacgcgtagc tcactaagcc cagcacgcgg ttttccgggt ttgccgccag cgcaatcgcc
    12541 accagcgcca ccaccagcac catcacacgt cctacccaca ccagctcttt ctggctggcc
    12601 tgtttacgca gaaacgcttt gtataaatct tcggtaatcg cactggagca caccagcagc
    12661 tggcaactta aggttgacat taccgccgcc agaatcgccg acagcagaat cccggcaatc
    12721 cacgggttaa acagaatttg cgccagttcg ataaacacgc gttcagcgtt ctggtttacc
    12781 gcacctgcta ccgccggatg ctcgttaaag taagcgatac caaagaagcc aacagccacc
    12841 gcccctgcca ggcaaaggat catccaggtc atactgatac gacgcgcatg gacaatgctg
    12901 tggtgagaat ctgccgccat aaaacgcgcc aggatgtgcg gctgcccgaa gtaacccagc
    12961 ccccaaccca tcaatgagat aatggcaaca aagttcagcc ctttgagcat atccacgttt
    13021 tcgatgctct tttgtttgat cacttccagc gagtcaccaa agccaccgac actgataatg
    13081 acgataaccg gcgtcaggat cagggcaaaa atcatcaggc tggcctgtac agtgtcagtc
    13141 cagctcaccg cgaggaaacc gccaataaag gtgtaaagga tcgtcgacgc agctcccgcc
    13201 aacagagccg tttcgtaact catgccaaag gtactttcaa acagacgcgc gcctgccaca
    13261 atgcccgaag cgcaataaat ggtgaagaac agcaaaataa ccagcgcgga gataatgcgc
    13321 aaaatgcggc ttttatcttc aaaacgcccg gtgaaataat ccggcaatgt taaggcgtta
    13381 ttattgtatt cggtatgcac acgcaaccgc ccggccacca gcttccagtt gatccacgcg
    13441 cctaatgtca ggccaatggc gatccagctt tcggaaatcc cggaaagaaa aaccgcgcca
    13501 ggcaacccca ttaacagcca gccgctcata tccgacgcac ccgccgataa tgccgtcacg
    13561 aatggcccaa gactacgacc gcccagaata tagtcgtcaa agtttttcgt tgatcgccag
    13621 gcgataaacc caatcaatat catgccaaag atatagacac aaaatgtcac caacatcggt
    13681 gtgctaatag ccatctaaag tctccaaaaa attattatcg gcaatgtcga aacttgccgt
    13741 tatatctgcc accggaacgg ggtaacagag tttatgtttt accagggcga ccgtatcctg
    13801 ccggaagcgc tggttattca caatcgattt aacacaccat ttacattaaa ttttagtgct
    13861 cagcgacact atttttcatc aggttgcact ctctcacatt ttttgcggtt gcacctttca
    13921 aaaaggttaa ctgccgcaga gaaaaagtct gagttatttt ttaatcactg tcatatcgat
    13981 ttcttttatt aacatttcat tcatttttaa gcttgctacg caagtcacat ttaacatggt
    14041 tgcacaaagt tgcaacatca tggatatttc acgataacgt taagttgcac ctttcagaac
    14101 aacaggagta atggcatggg aaccaccacc atgggggtta agctggacga cgcgacgcgt
    14161 gagcgtatta agtctgccgc gacacgtatc gatcgcacac cacactggtt aattaagcag
    14221 gcgatttttt cttatctcga acaactggaa aacagcgata ctctgccgga gctacctgcg
    14281 ctgctttctg gcgcggccaa tgagagcgat gaagcaccga ctccggcaga ggaaccacac
    14341 cagccattcc tcgactttgc cgagcaaata ttgccccagt cggtttcccg cgccgcgatc
    14401 accgcggcct atcgccgccc ggaaaccgaa gcggtttcta tgctgctgga acaagcccgc
    14461 ctgccgcagc cagttgctga acaggcgcac aaactggcgt atcaactggc cgataaactg
    14521 cgtaatcaaa aaaatgccag tggtcgtgca ggaatggtcc aggggttatt gcaggagttt
    14581 tcgctgtcat cgcaggaagg cgtggcgctg atgtgtctgg cagaagcgtt gttgcgtatt
    14641 cccgacaaag ccacccgcga cgcgttaatt cgcgacaaaa tcagcaacgg taactggcag
    14701 tcacacattg gtcgtagccc gtcactgttt gttaatgccg ctacctgggg gctgctgttt
    14761 actggcaaac tggtttccac ccataacgaa gccagcctct cccgctcgct gaaccgcatt
    14821 atcggtaaaa gcggtgaacc gctgatccgc aaaggtgtgg atatggcgat gcgcctgatg
    14881 ggcgagcagt tcgtcacggg tgaaaccatc gcggaagcgt tagccaatgc ccgcaagctg
    14941 gaagagaaag gtttccgtta ctcttacgat atgctgggcg aagctgcact gactgccgca
    15001 gacgcacagg cgtatatggt ttcctatcag caggcgattc acgccattgg taaagcgtct
    15061 aacggtcgtg gcatctatga aggtccgggc atttcaatca aactgtcggc gctgcatccg
    15121 cgctacagcc gcgcccagta tgaccgggta atggaagagc tttacccgcg tctgaaatca
    15181 ctcaccctgc tggcgcgtca gtatgatatt ggtattaaca tcgatgccga agaggccgat
    15241 cgtctggaga tctccctcga tctgctggaa aatctctgtt tcgagccgga actggcaggc
    15301 tggaacggca tcggttttgt tattcaggct tatcaaaaac gctgcccgtt ggtgatcgat
    15361 tacctggttg atctcgccac ccgcagccgt cgccgtctga tgattcgcct ggtgaaaggc
    15421 gcgtactggg atagtgaaat caagcgtgcg cagatggacg gccttgaagg ctatccggtt
    15481 tatacccgca aggtgtatac cgacgtttct tatctcgcct gtgcgaaaaa gctgctggcg
    15541 gtgccgaatc taatctaccc gcagttcgcg acgcacaacg cccatacgct ggcagcgatt
    15601 tatcaactgg caggtcagaa ctactatccg ggtcagtacg agttccagtg cctgcatggt
    15661 atgggcgagc cactgtacga gcaggtcacc gggaaagttg ccgacggtaa acttaaccga
    15721 ccgtgtcgta tttatgcccc ggttggcaca catgaaacgc tgctggcgta tctggtgcgt
    15781 cgcctgctgg aaaacggtgc taacacctcg tttgttaacc gtattgccga cacctctttg
    15841 ccactggatg aactggtcgc cgatccggtc actgctgtag aaaaactggc gcaacaggaa
    15901 gggcaaactg gattaccgca tccgaaaatt cccctgccgc gcgatcttta cggtcacggg
    15961 cgcgacaact cggcagggct ggatctcgct aacgaacacc gtctggcctc gctctcctct
    16021 gccctgctca atagtgcact gcaaaaatgg caggccttgc caatgctgga acaaccggta
    16081 gcggcaggtg agatgtcgcc cgttattaac cctgcggaac cgaaagatat tgtgggcttt
    16141 gtgcgtgaag ccacgccacg tgaagtagaa caggcgctgg aaagtgcggt taataacgcg
    16201 ccaatctggt ttgccacgcc tccggctgaa cgcgcggcga ttttgcatcg cgctgccgtg
    16261 ctaatggaaa gccagatgca gcaactgatt ggtattctgg tgcgtgaagc cggaaaaacc
    16321 ttcagtaacg ccattgccga agtgcgcgaa gcggtcgatt ttctccacta ctacgccgga
    16381 caggtgcggg atgatttcgc taacgaaacc caccgtccat tagggcctgt ggtgtgtatc
    16441 agtccgtgga acttcccgct ggctattttc accgggcaga tcgctgccgc actggcggca
    16501 ggtaacagcg tgctggcaaa accggcagaa caaacgccgc tgattgccgc gcaagggatc
    16561 gccattttgc tggaagcggg tgtaccccct ggcgtggtgc aattgctgcc aggtcagggg
    16621 gaaaccgtgg gcgcgcaact gacgggtgat gatcgcgtgc gcggggtgat gtttaccggt
    16681 tcaaccgaag tcgctacgtt actgcagcgc aatatcgcca gccgcctgga cgctcagggt
    16741 cgccctattc cgctcatcgc tgaaaccggc ggcatgaacg cgatgattgt cgattcttca
    16801 gcactgaccg aacaagtcgt cgtggatgtg ctggcctcgg cgttcgacag tgcgggtcag
    16861 cgttgttcgg cactgcgcgt gctgtgcctg caagatgaga ttgccgacca cacgttgaaa
    16921 atgctgcgcg gtgcaatggc cgaatgccgg atgggtaatc cgggtcgcct gaccaccgat
    16981 atcggtccgg tgattgatag cgaagcgaaa gccaatattg agcgccatat tcagaccatg
    17041 cgtagcaaag gccgtccggt gttccaggcg gtgcgggaaa acagcgaaga tgcccgtgaa
    17101 tggcaaagcg gcacctttgt cgccccgacg ctgatcgaac tggatgactt tgccgaattg
    17161 cacaaagagg tctttggtcc ggtgctgcat gtggtgcgtt acaaccgtaa ccagctacca
    17221 gagctgatcg agcagattaa cgcttccggc tatggtctga cgcttggtgt ccatacgcgt
    17281 attgatgaaa ccatcgccca ggtcactggc tcggcccatg ttggtaacct gtatgttaac
    17341 cgtaatatgg tgggcgcagt ggttggtgtg cagccgttcg gcggcgaagg gttgtccggt
    17401 accgggccga aagcaggcgg tccgctctat ctctaccgtc tgctggcgaa tcgcccggaa
    17461 agtgcgctgg cagtgacgct cgcgcgtcag gatgcagagt atccggtcga tgcacagttg
    17521 aaagccgcat tgactcagcc gctaaatgca ctgcgggaat gggcagcaaa tcgtccagaa
    17581 ttgcaggcgt tatgtacgca atatggcgag ctggcgcagg caggaacaca acgattgctg
    17641 ccggggccga cgggtgaacg caacacctgg acgctgctgc cgcgtgagcg cgtgttgtgt
    17701 attgccgatg atgagcagga tgcgctgact cagctcgccg ccgtgctggc ggtgggcagc
    17761 caggtactgt ggccggatga cgcgctgcat cgtcagctag taaaggcatt gccatcggca
    17821 gtcagcgaac gtattcaact ggcgaaagcg gaaaatataa ccgctcaacc ctttgatgcg
    17881 gtgatcttcc acggtgattc ggatcagctt cgcgcattgt gtgaagccgt tgctgcgcgg
    17941 gatggcgcaa ttgtttcggt gcagggtttt gcccgtggcg aaagcaatat ccttctggaa
    18001 cggctgtata tcgaacgttc gctgagtgtg aataccgctg ccgctggcgg taacgccagt
    18061 ttaatgacga tcggttaaac aggtagccgg aggatgttac aacctcctcc ggcatcttta
    18121 acgtggtcga atcccctcaa taataatccg ctgcacgttt tcaaccgttt gattgaaaaa
    18181 tatctcatcg cgcaacgtcg cgcctgtcac cgcctccacc tgaggggcga aatcggcgta
    18241 atgttgagtg gaagcccaaa tcatgaaaat caaatgctgc ggatcaatcg gcgcgagttt
    18301 gccgcttttg acccaaccgg caatcagcgc agatttctca tcaattaatg ccttcaaatc
    18361 gcccgtcagt tcatccatta acagcggcgc gcctgccagc atctccatac agaacaggcg
    18421 cgaagcctgc ggataatcgc gggagacttc cagcttcaga cggatgtact cttttatcgc
    18481 cgccagcgga gcgaaatctt cacgaaacgc ttttaacggt gccagccaga tatcgagaat
    18541 ctgccgcagc acggcaatat acagcgcctc ttttgacgga aagtaataca gcagattggt
    18601 ttttgaaaca cccgccaact ctgcgatctg ctccagcctt gtgccgtgaa aaccgaattg
    18661 tgaaaaagtg tccagtgctg cgctaagaat cgcttttttc ttcgcgctta ctgcgcgcga
    18721 acgtttaccc gttgttttca ctgcgccttg cgtcatgcgc tctcccctct tgtagattgg
    18781 caacaggata gcaaaacccc cgcccggcct cgaccttctg cactctcatc gcgcacagat
    18841 gcatgtttta tgtgcaactg ttttgaccgt ttagtccact ttttatcaga tattttagat
    18901 aacgtaatat taacatatta ataacattaa ctttttaaaa actggcatcc gctttgcaaa
    18961 caagccaata cgcggctgaa aaggatatgc aggatgcagc gccccgtctg
//
LOCUS       DAJVQG010000077        18618 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJVQG010000077 DAJVQG010000000
VERSION     DAJVQG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18618
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.077"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(28..1899)
                     /gene="ppiD"
                     /locus_tag="OQM83_002385"
     CDS             complement(28..1899)
                     /gene="ppiD"
                     /locus_tag="OQM83_002385"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCS1520630.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2091..2363)
                     /gene="hupB"
                     /locus_tag="OQM83_002386"
     CDS             complement(2091..2363)
                     /gene="hupB"
                     /locus_tag="OQM83_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCS1520631.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2572..4926)
                     /gene="lon"
                     /locus_tag="OQM83_002387"
     CDS             complement(2572..4926)
                     /gene="lon"
                     /locus_tag="OQM83_002387"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCS1520632.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5114..6388)
                     /gene="clpX"
                     /locus_tag="OQM83_002388"
     CDS             complement(5114..6388)
                     /gene="clpX"
                     /locus_tag="OQM83_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCS1520633.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6514..7137)
                     /gene="clpP"
                     /locus_tag="OQM83_002389"
     CDS             complement(6514..7137)
                     /gene="clpP"
                     /locus_tag="OQM83_002389"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCS1520634.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7383..8681)
                     /gene="tig"
                     /locus_tag="OQM83_002390"
     CDS             complement(7383..8681)
                     /gene="tig"
                     /locus_tag="OQM83_002390"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCS1520635.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9025..9342)
                     /gene="bolA"
                     /locus_tag="OQM83_002391"
     CDS             complement(9025..9342)
                     /gene="bolA"
                     /locus_tag="OQM83_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCS1520636.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9647..10225
                     /locus_tag="OQM83_002392"
     CDS             9647..10225
                     /locus_tag="OQM83_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1520637.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10269..11744
                     /gene="ampG"
                     /locus_tag="OQM83_002393"
     CDS             10269..11744
                     /gene="ampG"
                     /locus_tag="OQM83_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCS1520638.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12205..13152
                     /gene="cyoA"
                     /locus_tag="OQM83_002394"
     CDS             12205..13152
                     /gene="cyoA"
                     /locus_tag="OQM83_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HCS1520639.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13174..15165
                     /gene="cyoB"
                     /locus_tag="OQM83_002395"
     CDS             13174..15165
                     /gene="cyoB"
                     /locus_tag="OQM83_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HCS1520640.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15155..15769
                     /locus_tag="OQM83_002396"
     CDS             15155..15769
                     /locus_tag="OQM83_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCS1520641.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15769..16098
                     /locus_tag="OQM83_002397"
     CDS             15769..16098
                     /locus_tag="OQM83_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCS1520642.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16110..17000
                     /gene="cyoE"
                     /locus_tag="OQM83_002398"
     CDS             16110..17000
                     /gene="cyoE"
                     /locus_tag="OQM83_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCS1520643.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17185..17496
                     /locus_tag="OQM83_002399"
     CDS             17185..17496
                     /locus_tag="OQM83_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCS1520644.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            17480..18022
                     /locus_tag="OQM83_002400"
     CDS             17480..18022
                     /locus_tag="OQM83_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1520645.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(18078..>18618)
                     /locus_tag="OQM83_002401"
     CDS             complement(18078..>18618)
                     /locus_tag="OQM83_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCS1520646.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
BASE COUNT         4156 a         4901 c         4523 g         5038 t
ORIGIN      
        1 gcattttttt gcgaggcggc ttcaggatta ttgctgttcc agcgcatcgc caattttgat
       61 tttcgcctct ttacgcaggt tactcatcag agcttcaaag acgatttgtg cgttgttctg
      121 ggtgataccc tgcaccatcg cttttttctg atcttccggc attgaacctt gtttcacttc
      181 atccagcgcc agcagaacca cattaccttg catatcggtc gccataccgt agctcggttt
      241 gtctttcgct ggcagtggca gtgcaaacgc cgcctggcta atcgggtcac gaccggaacg
      301 gcttaaggtt ttcggctcgc caaatttcag accggcagcc tgcatagctt ccgccccttt
      361 gccggctttc aaatcaacca gcagtttctc agcatccact ttcgcctgtt gttcagcttt
      421 gttgtgctga accagtgcct taacttgttc ctgaacatct gccaacggtt tcaccgcttc
      481 cggtttgtgc tcgctgatgc gcagcacgaa tgcgcggtcg ccgtctacgg tgatgatgtc
      541 agagttgatg cccggcgcgc cgttttcacc taccagaccg ccgttaaaga tagcgtcggc
      601 aaccggcttg aagttcaact cttccggcag gttatctttg ctgaaccaac ccgtctgagt
      661 ggctttaacg ccggcagctt gctctgcacc ggccagagac tcggtgtcgt tgcttgccgc
      721 atcgctcact ttctgctgca gcgcatagta cgcatcgagg gctttttcgt gtttcacttt
      781 cgccgcaatg tcgtcacgta cttcgtctaa cgatttcact ttcgctggct gaatgtcgtc
      841 cagacgtaca atcaggaaac cgaccgaaga tttgatgaca ccagacagtt ggcctttttc
      901 tttcagacca gcatttttca gttcgtcggg gatagtggca tcttctaacc aacccatatc
      961 gccgccgtta cgggcagaga taatatcagc agatttttct ttggctaatg cagcaaaatc
     1021 accgccttta ttcagctcat caagtaccgc tttcgcttca tcttcagttt tggtctggat
     1081 gatgctgtag cgggtacgct gcggctgggt gaattgatcc tgatgctgat cgtagtagct
     1141 ctggatatcc gcatcgctaa ccggttgctg catcgttgcg gcatccagct tgatgtaact
     1201 cacgcggaat tgttccggcg tcatgaaatt gtttttgttt tgttcgtagt agctggcaat
     1261 ctcctgttcg gtcacaggct gcttcgccgc cagcgcgtta acatcgatag tcgcctcacg
     1321 caccacgcgt tgttgcgcga ccagtgccgc cagctcgtcg gtttcacctt tcagcataaa
     1381 atcggtaccg gcaacaccgt taatcagctg ttgggtagtg agctggttac gcagcgcctg
     1441 ggcgtactga tcggcggtca tccccatctg gttgaggata ccgttatagc ggctgttatc
     1501 aaatttgccg tcaacctgga aggctggggt cgcgaaaatc gcctgtttaa cctgctcatc
     1561 actgataccc agtttcagct cacgagcgta ctgatccaac agcgcctcgt cgatcagacg
     1621 attcagcacc tgttgacgca gggttttcat atagccttcg ttcgctgcca gctcggagta
     1681 ttgatcgccc agctgttgct gcatgcgatt acgctcgctg ttaaaggcgt tctcgaactg
     1741 cccacggctg atttcctggt cattcacttt tgcggcgtag ttattgcctc cgccaatcag
     1801 gtaaccactc acgccggtca atatgaacga cacgataatg ataccgaaaa taatcttgag
     1861 cacgagactg tttgcagccg tgcgtaagct gtccatcatg gtgtaacaac actccgctgt
     1921 agatgactgt gtacctcacg caacgtatcg atgcgcgcaa aggcctattg tgacaagaaa
     1981 cgggggcaat tgtcagccca caagacacag aaagtcaccg aatacaaata aaaaaggcac
     2041 atcagtagat gcgcccttga acttcgtcac atccccactg gggacaacgc ttagtttacc
     2101 gcgtctttca gtgctttacc tgcacggaag ctcggtactt tagcggcagc gatggtgatc
     2161 tctttaccgg tctgcgggtt gcggccagta cgggcagcac gctctttaac ggcaaaagta
     2221 ccaaaaccta ccagtgctac atcatcccct tctttcagag attcagttac ggaagcaata
     2281 atagcatcta acgcacggcc agccgcagct ttagagatat cagcccctgc agcaatcttg
     2341 tcgatcaatt gagatttatt cactcttctc ttcctcttta taatttatat cgcacttgaa
     2401 tccttcaagg tacgaacgcg cagcagttat atcaggccag ccatcccctt acaacacccc
     2461 ttaataaggg caagcccgat ccgccatcta acttagcgag acaaaaaaag gctggcaagc
     2521 ccgaattagc ctgccagccc tgtttttatt agtgcatttt gcgcgaggtc actattttgc
     2581 agtcacaacc tgcatgccag acggttcatt ttgcagcgcc agagtcagaa cttcctcaat
     2641 gcgcttcaca ggatgaatgt ccagatcggc aattacgttg tcaggaatct cttccagatc
     2701 gcgtttattt tcgaacggaa ttagcactgt tttaatcccg ccgcgatgcg ctgccaggag
     2761 tttttctttc aaaccaccga tcggcagtac ctgaccacgc agagtgatct caccggtcat
     2821 tgccacatcg gcacgaaccg ggttaccggt caggcaagaa accagcgcgg tgcacatagc
     2881 aataccggca ctcggaccat ctttcggcgt cgcaccttcc ggtacgtgga cgtggatgtc
     2941 acgtttttcg taaaaatcag ggttgatccc cagtttttcc gcacgcgcac gaaccaccgt
     3001 taacgccgcc tgaatagact cctgcatcac ttcgccgagc gaaccggtat aggtcagttt
     3061 gcctttaccc ggaacacatg cggtttcaat ggtcagcaag tcaccgccca cttccgtcca
     3121 cgccagaccg gttacctgac cgacacggtt ttcgttatca gcgcgaccat agtcgaaacg
     3181 ctgaacaccg aggtagtcat gcaggttatc gccgttaatt tcgatatgtt ttaatgactt
     3241 atcgagcagt aactgcttaa ccgctttgcg gcacagtttg gagatttcac gctccagacc
     3301 acgcacgccc gcctcacggg tgtagtaacg aataatgccg ataatggcgc tatcgtcgac
     3361 ggtcagctca ccttttttca gtgcattacg ttcaatctgc ttcggcagca ggtgacgttt
     3421 ggcgatgttc agtttttcat cttcggtata accggagagg cgaatcactt ccatacgatc
     3481 cagcagcggt gccggaatgt tcatggagtt cgacgtcgcg acaaacatca cgtcgctgag
     3541 atcgtaatcc acttccaggt agtggtcgct gaacgctacg ttctgctctg gatccagcac
     3601 ttcaagcagt gcagaggccg gatcgcctcg catgtcagaa gacattttgt cgatctcatc
     3661 gagcaggaac agcgggtttt tcacgcccac tttcgccatt ttctggatca gtttacccgg
     3721 catagaaccg atgtaagtac ggcggtgacc acggatttcc gcttcatcac gcacgccgcc
     3781 cagcgccata cggacatatt tacgcccggt ggctttggca atggactgac caagagaggt
     3841 tttacctacc cccggcggcc ctaccaggca gaggatcggt cccttgattt tgttgacacg
     3901 gctttgaacc gcaaggtact caaggatgcg atctttcacg cgctccagac cataatggtc
     3961 ggtatcaagg atttcctgcg cctgacgcag gtcttttttg accttgctgc gcgcattcca
     4021 cggtacctgt accatccagt cgatataacc acgcactacg gtcgcttctg ccgacatcgg
     4081 agacatcatt ttcagcttct gcaactctgc ttccgctttc tcttttgcct ctttcggcat
     4141 cttcgccgcg tcgattttgc gcttcagggc ttcgttttcg tccggcgcgt cgtccatttc
     4201 accgagttct ttctgaatag ctttcatttg ctcgttcaga tagtactcac gctgggattt
     4261 ctccatctgc tttttaacgc ggttgcgaat gcgtttctca acctgcagca gatcgatttc
     4321 cgattccatc attgccatca gatattccag acgttcgtta acgtcggaca tctccagaac
     4381 ggactgtttg tcagccagtt tcagcggcat atgtgcagca atggtatccg ccagacgcgc
     4441 cggatcgtcg atgctattca gcgacgtcag cacttctggt gggatttttt tgttcagctt
     4501 gatgtagcct tcgaactggc tgattgcagt acgcaccagc acttcctgtt cccgctcatc
     4561 aatggtcggc gactccagat actccgcctt cgcagaaaag tgttcgccat tgtcagagag
     4621 cgcagaaata cgcgcgcgct gtaacccctc gaccagcact ttgacggtgc cgtcaggcag
     4681 tttcagcatc tgcaatatag aggccacggt cccgacggtg aaaagatcgt ttacacccgg
     4741 ctcatccgtt gaagcttctt tctgcgcgac cagcataatt tttttatcat ggtccatcgc
     4801 cgcttccaga caacggatag atttttcccg cccgacaaat aaggggatga ccatgtgcgg
     4861 ataaaccacc acatcgcgca gcggcaatac ggggatttca atgcgttcag aacgctcagg
     4921 attcatagag ctctctctta gtttaatttc cgccaggtaa tcagatgaca cgactgtgct
     4981 tcacgccatc tattaacatg tacgtcagta tatggggatg tttcccccac attcaacgcc
     5041 gagaatagag gaaaaattaa aggggagata aaatcccccc tttttggtta actaattgta
     5101 tgggaatggt taattattca ccagatgcct gttgcgcttc cggcttacca taaatcagca
     5161 acggtttgct ttgaccatca attaccgact cgtcgataac cactttttcg acgtcttcca
     5221 tggacggcag atcgtacatg gtatcgagca gtgcggcttc tacgatggaa cgcaggccac
     5281 gggcaccggt tttacgcgcc atcgctttct tagcgatagc atccagcgcc tcgtcacgga
     5341 attccagatc cacgccttcc agattaaaca gcgcctgata ctgcttggtc agggcgtttt
     5401 tcggctcttt gaggatctga atcagagctt cttcgctcag ttcattcaac gttgctacaa
     5461 ccggcagacg accaatgaac tcagggataa gaccaaactt gatcagatct tccggttcaa
     5521 cctgcgccag cagctcgcct tcgcttgctt tgtcggactt cgcttttacc gtcgcgccaa
     5581 aaccaatgcc ggagccggtt tctacacggt gggaaatcac tttatccaga ccggcaaacg
     5641 caccgccaca aataaacagg atcttagagg tatcaacctg caagaattcc tgctgcggat
     5701 gtttacgccc accttgcggt ggaacagcag ctaccgtacc ttcgatcagt ttcaacagtg
     5761 cctgctgtac gccttcaccg gaaacgtctc gggtaatgga cgggttgtct gacttacgag
     5821 aaatcttgtc gatttcatcg atgtagacaa tcccgcgctg cgctttctgt acgtcgtaat
     5881 cgcacttttg caacagcttc tgaatgatgt tttcaacgtc ttcgcccaca taaccggctt
     5941 cggtcagcgt ggttgcgtcg gccatggtga acgggacgtc cagcaggcgc gccagcgttt
     6001 cagccagcag cgttttaccg gaaccggtcg gaccgatcag cagaatgtta cttttgccca
     6061 actcgacacc attgctggta tcgccgttgc gcagacgttt gtaatggttg tataccgcga
     6121 ccgccagcac ttttttcgcc tgttcctggc cgataacgta atcgtccagg tggttgcgaa
     6181 tttcatgcgg cgtcggtagc gcactgcgtt cacgatgcgg tgcaacttct ttaatctctt
     6241 cgcgaatgat gtcgttacat aaatcaacac attcgtcgca gatatacacg gatggaccgg
     6301 caatcagctt gcgcacttca tgctggcttt tgccgcaaaa agagcaatac agcaatttgc
     6361 ctgagccatc tttgcgttta tctgtcatgg gtcaaaacct cttctttgtt ctttgtgccg
     6421 cacacgacga cgcaaatgcc attctcaggc gcaagccgct tacagcgttg tgccgccctg
     6481 gataagtata gcggcacagt tgcgcctctg gcatcaatta cgatgggtca gaatcgaatc
     6541 gaccagaccg tattccaccg cttcaggggc ggaaaggaag cgatcgcgct cggtatcacg
     6601 ttcaatctgt tctaatgatt gacccgtatg aagcgccata agttcattca tgcgcccttt
     6661 aactttcaga atttcacggg catgaatttc gatatcggtc gcctggccct ggtagccgcc
     6721 caacggttgg tgaatcatca cgcgcgaatt cggcaggcaa aaacgtttac cttttgcccc
     6781 tgcggtcagc aagaaagcgc ccatcgaggc cgcctggccc atacagatgg tgctgacatc
     6841 aggcttgata aactgcatgg tgtcatagat agacatcccg gcagtgatca ccccgcctgg
     6901 ggagttaatg tacagataga tatctttttc tgggttttcc gcttctagga acagcatctg
     6961 cgccacaatc aggttcgcca tgtggtcttc aacctggcca gtcagaaaaa tgacgcgttc
     7021 cttaagtaga cgagaataga tatcaaaaga gcgctcaccg cgtgaggtct gttcaatgac
     7081 catcggcacc agcgccatat ggggtgcaaa gttatctcgt tcgccgctgt atgacatttc
     7141 cgtctcctgg ataaaattga aaaaacctgc tgtaccgatt gtaaccttat ggacggatta
     7201 tcagctagtc cctgtgttat gggtacggca tcaccatatt tcaagcataa caatcttttg
     7261 ttgttacgct aacaccgaaa gaggcttttc gcacggttcc atgcaaaatt catgacaaaa
     7321 aaaagcccac cacctggcgg tgacgggcct ttgtgcgaat ttagcgcgtt atgctgcgta
     7381 aattacgcct gctggttcat cagctcgttg aaagtggttt ctttttcagt cactttcgct
     7441 ttcgccagta cagcttcaac agcctgttct tccagagcaa cattgcgcat gttgtccatc
     7501 agttctttgt ttttgctgta gaactcgata acttctttcg gatcttcgta cgcagaagcc
     7561 atctcttcga tcaggccttt cacgcgctct tcgtcagctt tcagttcgtt ggtgcggata
     7621 acttcgccca gcagcaggcc aacaactacg cggcgtttag cctgttcttc gaacagttcg
     7681 cgcggcagtt ccagagcttg tttttcgttg ccaccgaaac gctgtgcagc ctggcgacgc
     7741 agaacgtcga tttcgctgtc gatcagcgca gccggtacgt cgatgtcgtt agcttttacc
     7801 agaccttcga tcgcctgaga cttaacgcgg ttacggatgg cgctcttcag ctcgcgctcc
     7861 atgtttttac gcacttcagc gcgcagacct tctacggaac catcttcaac gccgaaacgt
     7921 ttgatgaatt ctgcagtcag ttccggcagt tcacgctctt caactttctt caggttgata
     7981 gcgaatttcg ctgctttacc tttcagattt tctgcgtggt attcttccgg gaaggtcacg
     8041 tcgatggtga actcttcgcc agctttgtgg cctttgatac cgtcttcaaa gcccgggatc
     8101 atacgaccct ggcccatcgc cagtacgaaa tcagacgctt taccgccttc gaactcttcg
     8161 ccgtctacag aaccggtgaa gtcgatggtt acgcggtctt ctgcttcaac ggcgccgtct
     8221 ttttctttcc aggtcgcctg ctgtttacgc agagtatcca gcatgccgtc aacgtcagcg
     8281 tcggtcactt caacgatcgg tttttcaact tcgatcgctt ccagaccctg cagttcaact
     8341 tccggataaa cttcaaactc tacagagtag gtgaagtctt cacccagctt gtattcgccc
     8401 ggaacataag tcggtgcgcc agccggattg attttttctt taatgatggc gtcaatgaag
     8461 ttacggctca tcaggtcacc cagaacgtcc tggcgtacag acgcgccata acgctgagca
     8521 acgatattca ttggcacttt gcctttgcgg aagccgtcaa tacgtacttt tttcgcaacg
     8581 ttgaccagct cgcttttaac agcggtctcg atgctgtcag cagcgatagt aatcgttaca
     8641 cggcggccaa ggccttgagt ggtttcaact gaaacttgca tcttgttacc tcaaaaaatc
     8701 acagtgctcg gtcaactctt accccgcaag cacaggttgt tggcttcgcg gaaccgggat
     8761 gttcttataa tcaatcacat tccctgttac cagaatcatc ccgaaagcat tcaaatagga
     8821 cgcggcatta tagcggcatc acttttatga gtcgagaacg gttattgcgc gctgtcgcgt
     8881 attttttcac aattcccggg caattttcgt ctgaatgctg ggcaaaaagc gcaaaatcca
     8941 gacaaaacaa aacggcccgc aggccgtttt tcataaaatc tttagcaaca tactggaaaa
     9001 gcgccgacag ttgcaaatgc gtttttacgc gatgcttcct gctccacgac agggaggaga
     9061 ggcaaagacg gtgtcctgca acccttccca ctccttaata gtgtaagtat gcagagccag
     9121 cgcatgaacg gtagtagaga gttcctccgc taaagtactg taaatcattc gatgacgatt
     9181 cagaaaacgt tcacccgtaa aacgatcgct gaccagcaca actttaaaat ggctttcaga
     9241 gccggctggg acattgtgac gatagctttc atccactact tcgaggaata cgggttggaa
     9301 cgccgccctt aatttttctt ctatccgctc acgtatcatc atgaaatatc tcctccgaca
     9361 acgctgagat gtcatccatc cctttaaata ctagccgctt ttaccgtttc cattgcagct
     9421 taacaacaaa tatttaggtt tcgtcctggt ttttccgtca agcgtatgaa gtttactgat
     9481 ttaaagattg aaacacaggc ctcgcatcag cgtttctttt accaggggtg attatttcgc
     9541 caacatgatg aatttacatg ccttacccac ttcccctcgc cgttggcaat gttatgatgg
     9601 cgggaatttt taccaaacac gcttaagagt ctctgagagc ccgaacatgt tcaaaaaaat
     9661 cctcttcccg ttagttgctc tgtttatgct tgcaggatgc gcaaaaccgc caacaactat
     9721 tgaagtttcc ccgacgatta cactgccaca gcaggatcca agcctgatgg gcgtcaccgt
     9781 aagcattaat ggtgccgatc agcgtaccga tcaggcgctg gcaaaagtca cccgcgataa
     9841 tcaaatcgtt accctgaccg cctcccgaga tctgcgtttc ctgctgcaag aagtgctgga
     9901 aaaacagatg accgcgcgtg gttacatggt tggtccgaat ggcccggtta atctgcaaat
     9961 catcgttagc caactgtatg ctgacgtgtc ccagggcaac gtgcgctaca acatcgcgac
    10021 caaagcggat atcgccatca tcgctaccgc gcagaatggc aacaaaatga ccaaaaacta
    10081 tcgtgccagc tacaacgttg aaggtgcgtt ccaggcctcc aacaaaaata tcgccgatgc
    10141 ggttaacagt gtgctgaccg ataccatcgc tgatatgtct caggacacca gcatccacga
    10201 attcatcaag cagaacgcgc gttaatttct gccctctggc ccggtgcaaa ccgggcctgt
    10261 agacgcccat gtccagtcaa tatttacgta tttttcaaca gccgcgttca gccatattgc
    10321 tgatcctggg ttttgcttcc gggctaccgc tcgccctgac atccggtacc ttacaggcct
    10381 ggatgacggt cgagaatatt gatctcaaaa ccattggttt cttctctctg gtaggccagg
    10441 cttacgtttt taaattcctc tggtcaccgc tgatggaccg ctacacgccc ccattttttg
    10501 ggcggcggcg cggttggctg ctcgccacgc aaatcctgtt attagtcgcc attgcggcga
    10561 tgggttttct cgaacctggc acccaactcc gctggatggc ggcactggcg gtggtgatcg
    10621 ctttttgctc ggcctcccag gatattgtct tcgatgcgtg gaaaaccgat gtgctcccgg
    10681 cagaagaacg tggtgcgggc gcggcaatca gcgtgctggg ttaccgttta gggatgctgg
    10741 tttccggcgg cttagccctg tggctggcag ataaatggct gggctggcag ggcatgtact
    10801 ggttgatggc ggcgctgttg atcccttgta ttatcgcgac gttgcttgca ccagaaccga
    10861 ccgacaccat tcctgtgcca aaaacgctgg aacaagcggt tgttgcacct ctgcgagatt
    10921 tctttggtcg caataatgcc tggcttattt tgcttcttat cgtgctgtat aagctgggcg
    10981 acgcattcgc catgagcctg acaaccacgt ttttgattcg cggcgtcggg tttgatgcgg
    11041 gtgaagtagg cgtggttaac aaaacgcttg gcttactggc gaccattgtg ggcgcattgt
    11101 acggtgggat tttgatgcag cgcctgtcgc tgttccgggc gctgctgatt ttcggcattt
    11161 tacaaggtgc gtctaacgct ggttactggc tgctgtcgat tactgataag catctctaca
    11221 gcatgggcgc agccgtcttt ttcgaaaacc tctgtggcgg gatgggcaca tcagcctttg
    11281 tcgcactgtt aatgacgctg tgtaataagt cattttccgc tactcaattt gccctgctct
    11341 cagcgctttc cgctgtagga cgagtttatg tcggccccgt ggcgggttgg tttgttgaag
    11401 cacacggctg gtcgacattc tatctattct ccgtcgccgc tgccgtaccg gggcttattt
    11461 tgctgctggt ttgccgccag acgcttgaat atacacgagt aaatgacaac tttatctccc
    11521 gtaccgaata tccgacaggt tatgcctttg ccatgtggac actggcggcg ggcgtcagcc
    11581 tgttggccgt gtggttactg ctgttgacga tggacgcgct ggatttgacg cacttctctt
    11641 tcctgcctgc tctgctggaa gtcggggttt tagtcgccct ttctggcgtc gtgcttggtg
    11701 gtttgctgga ttatctggct ctacgaaaaa cgcatctgac gtaatctgta aatattattt
    11761 agctgtagta atcactcgcc gtaattatta cggctaaata aacatcagta tcgcttaata
    11821 atatttgttc ttttgtgcgg cttagcgttt ggaaattatt gacgcaattt atgaattcta
    11881 ttttttctta cacgattcag ctaatgagtc tttattttcc ccccacccaa ttgtcaccat
    11941 aatgataatt atttgttaaa caattgtttt attcaagatt ggttatacca attgcccgtc
    12001 caaactcctt ctgcactccc ctttcgttat aacgcccttt tgcaacagct tcttaaaatc
    12061 aacttgatat gttttgcaac atatgtaacc tgacagccaa atccaagtaa caggaattta
    12121 atcatgttta cagtaatgta acctccccgt aaaatgccca cacactttaa acgccaccag
    12181 atcccgtgga attgaggtcg ttaaatgaga ctcaggaaat acaataaaag tttgggatgg
    12241 ttgtcattat ttgcaggcac tgtattgctc agtggctgta attctgcgct gttagatccc
    12301 aaaggacaga ttggtctgga gcaacgttca ctgatactga cggcatttgg cctgatgttg
    12361 attgtcgtta ttcccgcaat cttgatggct gttggtttcg cctggaagta tcgtgcgagc
    12421 aataaagatg ctaagtacag cccgaactgg tcacactcca ataaagtgga agctgtggtc
    12481 tggacggtac ctatcttaat catcatcttc cttgcggtac tgacctggaa aaccactcac
    12541 gctcttgagc ctagcaagcc gctggcacac gacgagaagc ccattaccat cgaagtggtt
    12601 tccatggact ggaaatggtt cttcatctac ccggaacagg gcattgcaac cgtgaatgaa
    12661 atcgctttcc cggcgaacac tccggtgtac ttcaaagtga cctccaactc cgtgatgaac
    12721 tccttcttca ttccgcgtct gggtagccag atttatgcca tggccggtat gcagactcgc
    12781 ctgcatctga tcgccaacga acccggtact tatgacggta tctccgccag ctacagcggg
    12841 ccgggcttct caggcatgaa gttcaaagct attgcaacac cggatcgcgc cgaattcgac
    12901 caatgggtcg caaaagcgaa acagtcgccg aacaccatgt ctgacatggc tgcgttcgaa
    12961 aaactggccg cgcctagcga atacaaccag gtggaatatt tctccaacgt gaaaccagac
    13021 ttgtttgccg atgtgattaa caagtttatg gctcacggta agagcatgga catgacccag
    13081 ccagaaggtg agcacagtgc acacgaaggt atggaaggca tggacatgag ccacgcggaa
    13141 tccgcccatt aaaggggttg aggaagaata aagatgttcg gaaaattatc acttgatgca
    13201 gtcccgttcc atgaacctat cgtcatggtt acgatcgctg gcattatttt gggaggtctg
    13261 gcgctcgttg gcctgatcac ttacttcggt aagtggacct acctgtggaa agagtggctg
    13321 acctccgtcg accataaacg cctcggtatc atgtatatca tcgtggcgat tgtgatgttg
    13381 ctgcgtggtt ttgctgacgc cattatgatg cgtagccagc aggctcttgc ctcggcgggc
    13441 gaagcgggct tcctgccacc tcaccactac gatcagatct tcaccgcgca cggcgtgatt
    13501 atgatcttct tcgtggcgat gcctttcgtt atcggtctga tgaacctggt ggttccgctg
    13561 cagatcggcg cgcgtgacgt tgcgttcccg ttcctcaaca acttaagctt ctggtttact
    13621 gttgttggtg tgattctggt taacgtttct ctcggcgtgg gcgaatttgc gcagaccggc
    13681 tggctggcct atccaccgct atcgggaata gagtacagtc cgggagtcgg tgtcgattac
    13741 tggatatgga gtctccagct atccggtata ggtacgacgc ttaccggtat caacttcttc
    13801 gttaccattc tgaagatgcg cgcaccgggc atgaccatgt tcaagatgcc agtatttacc
    13861 tgggcatcac tgtgcgcgaa cgtactgatt attgcttcct tcccaattct gacggttacc
    13921 gtcgcgttgt tgaccctgga tcgctatctg ggcacccatt tctttaccaa cgatatgggt
    13981 ggcaacatga tgatgtacat caacctgatt tgggcctggg gccacccgga agtttacatc
    14041 ctgatcctgc ctgttttcgg tgtgttctcc gaaattgcgg caaccttctc gcgtaaacgt
    14101 ctgtttggtt atacctcgct ggtatgggca accgtctgta tcaccgtgct gtcgttcatc
    14161 gtttggctgc accacttctt tacgatgggt gcgggcgcga acgtaaacgc cttctttggt
    14221 atcaccacaa tgattatcgc catcccgacc ggggtgaaga tcttcaactg gctgttcacc
    14281 atgtatcagg gccgcatcgt gttccattct gcgatgctgt ggaccatcgg ttttatcgtc
    14341 actttctcgg tgggcgggat gactggcgtg ctgctggcag taccgggcgc ggacttcgtt
    14401 ctgcataaca gcctgttcct gattgcgcac ttccataacg ttatcatcgg cggcgtggtc
    14461 ttcggctgct tcgcagggat gacctactgg tggcctaaag cgttcggttt caaactgaat
    14521 gaaacctggg gtaaacgcgc gttctggttc tggatcatcg gcttcttcgt tgcctttatg
    14581 ccgctgtatg cgctgggctt catgggcatg acccgtcgtt tgagccagca gattgacccg
    14641 cagttccaca ccatgctgat gattgcagct agcggtgcag tactgattgc gctgggtatt
    14701 ctctgcctcg ttattcagat gtacgtttct attcgcgacc gcgaccagaa ccgtgacctg
    14761 actggcgacc cgtggggtgg ccgtacgctg gagtgggcaa cctcttcccc gcctccgttc
    14821 tataactttg ccgttgtgcc gcacgttcac gagcgtgatg cattctggga aatgaaagag
    14881 aaaggcgaag cgtataaaaa gcctgaccac tatgaagaaa ttcatatgcc gaaaaacagc
    14941 ggtgcaggta tcgtcattgc agctttctcc accatcttcg gtttcgccat gatctggcat
    15001 atctggtggc tggcgattgt tggcttcgca ggcatgatca tcacctggat cgtgaaaagc
    15061 ttcgacgagg acgtggatta ctacgtgccg gtggcagaaa tcgaaaaact ggaaaaccag
    15121 catttcgatg agattactaa ggcagggctg aaaaatggca actgatactt tgacgcacgc
    15181 gactgcccac gcgcacgaac acgggcacca cgatgcaggc ggaaccaaaa tcttcggatt
    15241 ttggatctac ctgatgagcg actgcattct gttctcaatc ttgtttgcta cctatgccgt
    15301 tctggtgaac ggcaccgcag gcggcccgac aggtaaggac attttcgaac tgccgttcgt
    15361 tctggttgaa actttcttgc tgttgttcag ctccatcacc tacggcatgg cggctatcgc
    15421 catgtacaaa aacaacaaaa gccaggttat ctcctggctg gcgttgacct ggttgtttgg
    15481 tgccggattt atcgggatgg aaatctacga attccatcac ctgattgtta acggcatggg
    15541 tccggatcgc agcggcttcc tgtcagcgtt ctttgcgctg gtcggcacgc acggtctgca
    15601 cgtcacttcc ggtcttatct ggatggcggt gctgatggta caaatcgctc gtcgcggcct
    15661 gaccagcact aaccgtaccc gcatcatgtg cctgagcctg ttctggcact tcctggatgt
    15721 ggtttggatc tgtgtgttca ctgttgttta tctgatgggg gcgatgtaat gagtcattct
    15781 accgatcaca gcggcgcgtc ccatggcagc gtaaaaacct acatgacagg ctttatcctg
    15841 tcgatcattc tgacggtgat tccgttctgg atggtgatga caggagctgc ctctccggcc
    15901 gtaattctgg gaacaatcct ggcaatggca gtggtacagg ttctggtgca tctggtgtgc
    15961 ttcctgcaca tgaataccaa atcagatgaa ggctggaaca tgacggcgtt tgtcttcacc
    16021 gtgctaatca tcgctatcct ggttgtaggc tccatctgga ttatgtggaa cctcaactac
    16081 aacatgatga tgcactaaga gcggcggtta tgatgtttaa gcaatacctg caagtaacga
    16141 aaccaggcat catctttggc aacctgatct cggtgattgg gggattcctg ctggcctcaa
    16201 agggcagcat tgattatccc ctgtttatct acacgctggt tggggtgtca ctggttgtgg
    16261 cgtcgggttg tgtgtttaac aactacatcg acagggatat cgacagaaag atggaaagga
    16321 cgaagaatcg ggtgctggtt aaaggcctga tctctcctgc tgtctcgctg gtgtacgcca
    16381 ccttgctagg ctttgctggc tttatgctgc tgtggtttgg cgcgaatccg ctggcctgct
    16441 ggctgggggt gatgggcttt gtggtttatg tcggcgttta tagcctgtac atgaaacgcc
    16501 actcggtcta cggcacattg attggttcgc tctccggcgc tgcgccgccg gtgattggct
    16561 actgtgcagt aaccggcgag ttcgatagcg gcgcagcgat cctgctggct atcttcagcc
    16621 tgtggcagat gcctcactcc tatgccatcg ccattttccg ctttaaggat tatcaggcgg
    16681 caaacattcc ggtattgcca gtggtaaaag gcatttcggt ggcgaagaat cacatcacgc
    16741 tgtatatcat cgcctttgcc gttgccacgc tgatgctctc tcttggcggt tacgctgggt
    16801 ataaatatct ggtggtcgcc gcggcggtga gcgtctggtg gttaggtatg gccctgcgcg
    16861 gttataaagt tgctgatgac aggatctggg cgcgcaagct gttcggcttc tctatcatcg
    16921 ccatcacagc cctctcggtg atgatgtccg ttgattttat ggtgccggac tcgcatacgc
    16981 tgctggctgc tgtttggtaa cacaacctct ctattaaaaa ggtgctacgg cacctttttt
    17041 attgtctgag atcagagata cggctaataa ccgtatgtct tgacagagtg agcagatgaa
    17101 cataaattaa tcatacggtt gatagccgta ttatgtatga gatcgactcc ctacgatttc
    17161 attcagttaa tcaaagaggt cataatgcct ggaaaaacag ccactctacc cgatgtcgat
    17221 aaaacgctga aaaatgcgcg ggtcgaactc aaaactagtc ccgacgccaa aaacaaactt
    17281 cgtgaagctg cgcaagccgt tggggtagat ttaagcgctt ttattttgag cgctgctatg
    17341 gaacgcgccg aaagtgtgct tgataaccaa cgtcgccgtg agctttcgaa tcaaagctgg
    17401 gaactgatga accaactcat cgctgaacct gctcaaccga cgctcgccct caaggcgtta
    17461 atgaaaagga aaaacagcga tggtcgacaa gcatgaagag attactctgc ccatagtcct
    17521 ctcctgtaat tatcagtctg atattactta tcctgggcaa aaacagtttg attgcggtaa
    17581 ccctgttatc gataaatttg tacgcgcatc gctaaagaaa agtgtgcgta atagcgactg
    17641 tgcggctaaa gcacttattg acagacaaag tggtgaactg atcggcatct gtacttttac
    17701 ggcatattcg ctggaaaaac aacgcgtttc tggcgtcctt cagggttcac aaccttcaga
    17761 aattggtgtt gtcagattag tcatgttggg ggtagcacgg aagtatcaaa agcggggctt
    17821 tggtcaggac ctactatgtg atttttttga acatgtaaaa ataattcacc aggcattacc
    17881 aattaaaggg gtttatcttg atgctgaccc tgccgccatt aatttttatg ctcgtctcgg
    17941 ctttgttcag ctttcagcga caccaaatgc ttttggtgct gtacctatgt ttttggcgat
    18001 tcagcatatt ctcgcggctt agattattat cgccacccgc cagggcaagt tttgccttag
    18061 ccgggggctg agcagcgtta ccacttttgt tcaattgccc ccgctttcag caactccaca
    18121 tccttgccgt taatggttgc cgttgcggca acgattgtgg tccttacagt tgcacaagat
    18181 tttcgctcaa attccggttt ataaagaata agagacgaat tactggtaaa tgaaccaaat
    18241 aggtaatcag taaagctaat attcagccgg tttaaaccga aaggcgcgaa gccaaccgtt
    18301 tggttaatct ccttaccgga tgggcgacga atgctgaatg tcacgcgaag attggccact
    18361 tcgcctaatt tattggtggt ggcgaagagg ctatccatgc aggaaaaacc tttatctaaa
    18421 aaattatctc gcatcccaga acgggtaacc gtaatcacgg gtaacgtatc gtcaaccgtt
    18481 tttctgtcaa tcatgtcttt tcgacaagta gccagaaagt cttccgggcc accataaatg
    18541 tggacacctt tatcaagata ttccttcgct ttcttacaat ctacctcaac atactgacca
    18601 ttggcgtaca taatgccc
//
LOCUS       DAJVQG010000078        18524 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJVQG010000078 DAJVQG010000000
VERSION     DAJVQG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18524
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.078"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..1711
                     /locus_tag="OQM83_002402"
     CDS             <1..1711
                     /locus_tag="OQM83_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCS1520647.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1712..3361
                     /locus_tag="OQM83_002403"
     CDS             1712..3361
                     /locus_tag="OQM83_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCS1520648.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3366..4142
                     /locus_tag="OQM83_002404"
     CDS             3366..4142
                     /locus_tag="OQM83_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCS1520649.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4216..5400)
                     /gene="atoB"
                     /locus_tag="OQM83_002405"
     CDS             complement(4216..5400)
                     /gene="atoB"
                     /locus_tag="OQM83_002405"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HCS1520650.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5431..6753)
                     /locus_tag="OQM83_002406"
     CDS             complement(5431..6753)
                     /locus_tag="OQM83_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HCS1520651.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6750..7400)
                     /gene="atoA"
                     /locus_tag="OQM83_002407"
     CDS             complement(6750..7400)
                     /gene="atoA"
                     /locus_tag="OQM83_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HCS1520652.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7400..8062)
                     /gene="atoD"
                     /locus_tag="OQM83_002408"
     CDS             complement(7400..8062)
                     /gene="atoD"
                     /locus_tag="OQM83_002408"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HCS1520653.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8258..9643)
                     /gene="atoC"
                     /locus_tag="OQM83_002409"
     CDS             complement(8258..9643)
                     /gene="atoC"
                     /locus_tag="OQM83_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HCS1520654.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(9640..11466)
                     /gene="atoS"
                     /locus_tag="OQM83_002410"
     CDS             complement(9640..11466)
                     /gene="atoS"
                     /locus_tag="OQM83_002410"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HCS1520655.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            11633..14482
                     /gene="rcsC"
                     /locus_tag="OQM83_002411"
     CDS             11633..14482
                     /gene="rcsC"
                     /locus_tag="OQM83_002411"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCS1520656.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(14682..15332)
                     /gene="rcsB"
                     /locus_tag="OQM83_002412"
     CDS             complement(14682..15332)
                     /gene="rcsB"
                     /locus_tag="OQM83_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCS1520657.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(15349..18021)
                     /gene="rcsD"
                     /locus_tag="OQM83_002413"
     CDS             complement(15349..18021)
                     /gene="rcsD"
                     /locus_tag="OQM83_002413"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCS1520658.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEKIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         4526 a         4534 c         4867 g         4597 t
ORIGIN      
        1 tgagatgcgc cagacgctga cgctgcacaa aggggcgaat tatatttcgc tggcgcagaa
       61 cattcagcaa tctggcttgt taagcgcaga actgcaacaa aatgggcaag tgcaggacag
      121 cattagcaca aaactgtctt ttgtggataa cagctggccc gttgaacagc agaaaaatgt
      181 catgctcggc ggtggcgaga acgcgctgat gttgcccgag caggctagca atatccggct
      241 acaaagtagc gaaacgccgc aggagatttt ccgcaacaat cttgatgcgt tagtcgatgg
      301 accatggggt ggcgtgatca acaccggtag ccgtctgatc ccgctcagtc tcgcctggcg
      361 ttcgcttgcc gatcatcaaa gtgccgccgc taacgacatt cgtcagatga ttcaggataa
      421 ccgtctgcgg ctgatgcaac tggcggggcc cggagcgcgc tttacctggt ggggtgaaga
      481 tggcaatggt gacgccttcc ttacggcatg ggcatggtac gccgactggc aggccagcca
      541 ggcgctcggc gtaacgcaac aaccggaata ctggcagcat atgctcgaca gttatgccga
      601 gcaggcggat aacatgccgt tattgcatcg ggcgctggtg ctggcgtggg cacaggagat
      661 gaatctgccg tgcaaaacgt tgttgaaagg gttggatgaa gctatcgccc ggcgcggaac
      721 taaagatgaa gatttctctg aggaagacat ccgcgatatc aatgacagcc tgatcctcga
      781 tacaccggaa tctccactgg cagatgcggt ggcaaacgtc ttaaccatga cgttgctgaa
      841 aaaagcgcag ttgaagtcca cggtgatgcc acaggttcag caatatgcgt gggataaagc
      901 ggcaaacagc aatcagccgc tggcgcacac ggttgtgctg ctcaatagcg gtggtgacgc
      961 tacccagacg gccgctattt taagtggttt gaccgctgag caatccacta ttgagcgcgc
     1021 gctggccatg aactggctgg cgaaatatat ggcgacaatg cctccagttg ttttgcctgc
     1081 gcctgcgggc gcatgggcta aacataagtt aactggaggg ggcgaagact ggcgttgggt
     1141 tggtcagggt gtgccggaca ttctctcttt tggtgacgaa ttatcgccgc aaaatgtgca
     1201 ggtccgctgg cgtgaaccgg caaaaacggc tcaacaaagt aacattccgg tgaccgttga
     1261 acgccagttg tatcggctta tccccggtga agaagagatg agctttactc tgcagccggt
     1321 gaccagcaat gagattgaca gcgatgcgct gtatctcgat gaaattacgc ttaccagcga
     1381 gcaggatgca gttctgcgct atggtcaggt ggaagtaccg ctcccgccgg gagccgacgt
     1441 tgagcgcaca acatggggca tttcagtcaa taaacccaac gccgcgaaac agcaggggca
     1501 attgctggaa aaagcgcgta atgaaatggg cgaactggcc tatatggtgc cggtgaaaga
     1561 actgacggga acggtcactt tccgccattt gctgcgcttc tcgcaaaaag ggcaattcgt
     1621 tctgcctcct gctcgttatg tgcgttccta tgcacctgcg cagcaaagtg ttgcggcagg
     1681 gagtgaatgg accgggatgc aggtgaaata agtgaactgg cgcagaatcg tctggctatt
     1741 ggcgttggtt actctgccaa cgcttgcgga ggagacgccg ttacaactgg tgctgcgcgg
     1801 tgcgcagcac gatcaactgt atcagctgtc gtcatcaggc gtgaccaaag tctcagcatt
     1861 acctgactcg ctcaccacgc cgttagggag tttgtggaaa ctgtatgttt acgcctggct
     1921 ggaggatacc catcaaccgg agcagcccta ccagtgtcgc ggaaactccc cggaagaggt
     1981 ttattgttgt caggctgggg agagcataac gcgcgatacc gcgctggtac gttcctgtgg
     2041 gctctatttt gcgccgcagc ggttgcatat tggcgcagat gtgtgggggc aatactggca
     2101 acagcgtcag gcacctgcgt ggttagcgtc tcttacaacg ctgaagccag aaacctcggt
     2161 aacggttaaa tcgctgctcg attcattagc gacgttacct gcgcaaaaca aagctcagga
     2221 agtcttactg gatgtggtgc tggacgaggc gaaaatcggt gtcgcatcca tgctcggtag
     2281 tcgggtacgg gtgaaaacct ggagctggtt tgccgatgat aaacaggaaa tccgccaggg
     2341 tggttttgct ggctggttaa ccgatggtac accgctgtgg gttactggca gtggcaccag
     2401 caaaacggta ctaacccgtt atgcgacggt gcttaatcgg gttttgccag tgccgacgca
     2461 ggttgccagt gggcaatgtg tagaggtgga gctgtttgcc cgctatccgc tgaagaaaat
     2521 cacagcggaa aagagtacga cggcagtaaa acccggcgta cttaatgggc gctatcgtgt
     2581 gacgtttact aatggtaacc acattacgtt tgtcagccac ggtgagacga ccttactgag
     2641 cgaaaagggc aaactgaaat tgcagtcgca tctcgatcgt gaagagtacg ttgccagagt
     2701 tctggatcgc gaggcgaaaa gtacgccacc tgaggcagcg aaagccatga ccgtggcgat
     2761 acgtactttt ttacagcaaa acgccaatcg ggaaggtgat tgcctgacca tccccgacag
     2821 cagcgccacg cagcgtgttt ctgcttcgcc agcgaccacg ggggcgcgaa cgatgaccgc
     2881 ttggacgcag gatctcatct atgcaggcga tccggttcat tatcatggca gtcgcgctac
     2941 cgaagggaca ctttcctggc ggcaagccac ggcccaggcg gggcaggggg agcgttacga
     3001 ccagatactc gcgtttgctt atcccgacaa cagccttagc cgctggggag ctccacgctc
     3061 aacctgccag ttattaccca aagcgaaagc ctggctggcg aaaaaaatgc cgcagtggcg
     3121 gcgtatatta caaggcgaga cggggtacaa cgaaccagac gtgtttgcgg tctgtcgttt
     3181 agtctcaggt ttcccctata ccgatcggca gcagaaacgg ctgtttattc gtaacttctt
     3241 tacgcttcag gatcggctgg atttaaccca tgagtatctg cacctggcct ttgatggtta
     3301 tcccaccggg cttgatgaga actatatcga aacgctgacc cgacaattat taatggacta
     3361 atgctatgcg aaagattttt cttccgttgt tactggtggc actttcgcct gtcgctcaca
     3421 gtgaaggtgt gcaggaagtc gagattgacg caccgctctc tggctggcat ccggcagagg
     3481 gcgaagatgc cagtttttca caatcgatca attacccggc gtcgtccgtc aacatggcag
     3541 atgatcaaaa tatctcagcg cagatccgcg gcaaaataaa gaattatgct gcggcgggta
     3601 aagttcagca gggccggctg gtggtcaacg gtgccagtat gccgcagaga attgaatccg
     3661 atggttcatt tgcacgccct tatattttca ctgaaggcag caacagcgtg caggtcatca
     3721 gcccggatgg gcaaagccgg caaaaaatgc agttttactc aacgtcgggt ataggaacga
     3781 ttcgtgcacg tttacggctg gttctctcgt gggatacgga caataccgat ctcgatctcc
     3841 atgttgtcac gcccgatggc gaacacgcct ggtacggtaa caccgtgctg aaaaacagtg
     3901 gtgcactgga tatggatgtc acgacggggt acggacccga gattttcgcc atgccagcac
     3961 cagttcacgg gcgctatcag gtgtatatca actactatgg tgggcgcagc gaaacggaat
     4021 tgacgaccgc ccaactgacg ctgatcaccg atgaaggatc ggtcaatgag aaacaggaaa
     4081 catttattgt accgatgcgt aatgctggcg agctgacgct ggtgaaaagt tttgactggt
     4141 gattaatggg gttaccttcg ccgtcgatgt tcaagaaaac gcccgataac tttcgctatc
     4201 gggtgttttt attgattaat tcaaccgttc aatcaccatc gcaattccct ggccgccacc
     4261 aatgcacagt gttgccagcc ccagcgtttt atcgcgtgcc tgcatggcat gtaatagtgt
     4321 gaccagaata cgagcaccac tggcaccgat aggatgcccg agcgcgatgg ccccgccgtt
     4381 gacattcact ttctcagaat caaagcccag ggttttccca acggcgagga actgtgcagc
     4441 aaatgcttca ttagcttcga tgagatcaat gtctgccagt tgcagccccg ccagttgtaa
     4501 cgctttttgc gtggcaggta ctggccccat acccatcaat gcgggaggca cgccaccgct
     4561 ggcataactt ttaatgcgag ccaggggggt aaggcctgct gccagcgccg cagattcttc
     4621 cataatcacc agagcggcag caccgtcgtt aataccagac gcgttcccag cggtgactgt
     4681 tcctgcttta tcgaaggccg ggcgcaatgc acctaacgct tcagccgttg aattcgcttt
     4741 cgggaattcg tcttgactga agacgaaggt tttcttccga gtgacaacat ttaccgggac
     4801 gatttcggct gtaaaagcac cggactcaat tgcggctgcc gctttacgct gtgaatgtag
     4861 cgccagttca tcctgcattt cacgggtaat tccgtactct ttagccacgt tttcggcggt
     4921 aatccccata tgataaccat gggtggcgca catcaggcca tcgcgcagga ttacgtcata
     4981 aacctgtccg tctccaagac gataaccaga gcgtgctttt gcatcgagta agtagggcgc
     5041 taaactcata ttttccatac cccccgccac aatgctcttc gcctgacctg cctgaattgc
     5101 ctgggcggca agcgccacac ttttaagacc cgaaccacac actttattga ccgtgaatcc
     5161 gcacaccgtt tctgccagcc cgctttttaa cagtgcctga cgtgccggat tttgccccaa
     5221 cccggcttgc aacacgttac ccataatcac ttcatcaatg tgtagtgaat cgatttttgc
     5281 acgttcaatg gcggctttaa ttactgtcgc ccccaggtcg atggcgctgg tggaagcgag
     5341 tgaaccgtta aaactaccga tagcagtacg taccgcactg acgatgacac aatttttcat
     5401 tttatattcc ttctgtctgt agggttgccg tcagaacagc gttaaaccaa tgacgaaaat
     5461 gacgccggag aagagcaggg cagtgatgca atagcccatg atgtcgcgga caccgagtcc
     5521 ggcgattgcc agtgctggca gcgcccagaa tggttgttcc atgttcatcc attgctcgcc
     5581 gtaggcgatc gccattaccg atttaccgag atcagcgccc agcgcctggg ctgcgggtat
     5641 cacgaaaggt ccctgaataa cccagtgacc gccgccagac ggaacggcga agttaatcag
     5701 tgcagaacta aaaaaggtca ttaccgggaa ggtgtctttg ttcgcaacat tgatgaagaa
     5761 ttcggtaatg agtccaccca gaccagaatg ctccatcatc agttggatcc cagcgtagaa
     5821 ggggaattgc accagaatac cggcagtact gcgtgctgcc gcgctgatag cacgcatata
     5881 agccattggc gttttatgta gcaacagacc cgcaatcata aacatcaggt tgacggtatt
     5941 gatggtgatg ttgaagccat gttcgctgaa gtacatcgcc aggtaagcga taccgagtgc
     6001 gccgatgatc aacgtcagaa tgcggctttc ttccagtcgc tcggatggtg gggcatcttt
     6061 cggtagctgc ttttgaaaat cagcctcttc catgagtagt tccggatcga tactcaccac
     6121 gtcagacggt tttggcatca tcatgcgggt gataaatggc atcaccacaa tcaacgtcac
     6181 agtgatgaaa atgttaaaac cactgaacag agtatcgccc accgggatca gcccggcgat
     6241 atgctcaacc gggttgcccg gtgttgcagc caacagaggc attgatccag agaagccgcc
     6301 accccaggtg agaaaaccaa tgtaggcgca ggcaatgagc aacggataat cagaaccggg
     6361 gactcgccgg gcgacttcac gggcaaacat tgcgccgaca accaaaccaa atccccagtt
     6421 gatgacacaa gcgactgaac cgaagaaagt gaccagcatg acgccctgta cgggcgtctt
     6481 tgcggcggag gcggcagtac gcagcaaact tttcaccgga gcagagctgg caagggcatg
     6541 accggtaacg atgataagcg ccatctgcat accaaacgcc agcaagttcc agaaaccgtc
     6601 accccacatt ttcaccatgc tgatcggcgt ttgtggtgtt aaccaaagcg cgatcacgaa
     6661 tgttagcaat gtcagcaaca tggcaaagat cagtggatcg ggaagccatc ggctgacaaa
     6721 acgcgtcata aaacgcgata tgcgaccaat cataaatcac cccgttgcgt attcagatcg
     6781 gcggcgactt caaaccgagc ttctgttttg gcacgcacgg tggctaaatc acacccgtcg
     6841 gcaatttcgg tgagccacat tttgccgtca ataaaacgaa agacagccag ttcagtaacc
     6901 agcatatgca ccgcatgttg cgcagtgagt ggcatggtgc agctgcgcaa aatttttgct
     6961 gaaccatctt tggcgcaatg ttccatggcg atgatcactt tgcgcgaccc ggtcaccaga
     7021 tccatcgcgc cacccatacc gggcaccatt ttcccaggca ctacccagtt cgcgaggttt
     7081 gcttcttcgt ctacttgcaa accgccgagc acgcaggcat caatatgacc gccacggatt
     7141 agcgcaaatg acatggcgct atcaaacatg gctgcaccgg gtaaaacacc gcacggttgc
     7201 ccgccagcgt tcaccagatc tggatgcgct gtcgtgaccg ggcctaaacc gaggaagccg
     7261 ttttccgatt gcagagtgat atgaataccc tccggtaaat aattggcgac cattgtgggt
     7321 aaaccgatcc ctaagttaac gatgtcacca tcacgaagct cttgcgccac acggcgcgca
     7381 atacgttgtt tcgcatccat tatttgctct cctgtgaaac gatgatgtgg tcgataacgg
     7441 caccaggggt gacaatatgg tcaggttgca gctcgccggt ttcgaccagt tcatctggct
     7501 ctaccagcgt gatatcagcc gcaagggcta tcagggggtt aaagttgcgg gcgctaagtt
     7561 gataggtcag gttgccaagt gtgtcgcaac gatgagcgcg aattagcgcc aggtcggcgc
     7621 gcagtgggcg ttcgagcagc caggttttac cgtcgagtgt cagtgtctgt ttgccttcct
     7681 ctacgacggt gccgacaccc gttggggtga gaaaaccacc aagtccagct ccaccacagc
     7741 gaatttgctc gattagcgta ccttgcggca ccagaacgac gtccatctca ccagatatca
     7801 tgcgccgacc tgtttccggg ttggtgccga tatgtgaagc aatcactttg cggactcgac
     7861 cattgacgat gagcggaccg atgccggtat caacaaacgc ggtatcattg gctatcaatg
     7921 tcaggtcgcg aacaccagat tccagtaatg cttcaaccag gcgggatgga gtgccaatcc
     7981 ccataaatcc gcccaccatg atggtcatgc cgtcacgaaa gaagccggtg gcgtcttgta
     8041 atgtcatcaa ttttgttttc attttttatc cctcttgcat acaccgttgt gggtacagtc
     8101 aggcaatagc aaggggagtg ccagaatgag attattggat ttagttattt aactaattga
     8161 atatgaaggg ataaattgga tggggattta taaggagaat gagcggctat gcagaaaatt
     8221 gcgcactgtg caaatttctg catagcaagt tttggtgtta tacatccgcc ggatcgatac
     8281 cgtattcctg gagtttatac atcaatgcac ggcgactgat gcccagcatt aaagcagtgc
     8341 gggttcggtt tccttcttgt tgttccagca cttccataat gatgcgtttt tcgacgcgtt
     8401 taatttcctc ttttaaatta cgctcaccga cagaggctgt ttttacctcg ccagcattac
     8461 agactggctg acgaatctgt ggcggaagat cctcagaaaa aatgatcggg cctgaattca
     8521 tcacgacggc gcgttcaata acgttagaaa gctctcgaat atttcccggc catgaccagg
     8581 cggtaagcag tgacattgcc atcggatcga tgtcgataat atcgcgctga ttctcactac
     8641 tgaatttttg caaaaagtga ttagctaaca gggaaatatc ttcccggcga tcgcgcagag
     8701 gcggcagtat taaatgaata acgttaaggc gataaaagag atcttcacgg aaggtgcctt
     8761 cttttaccat tgcctgcaag tcacggttgg tggcagcaat gatgcggata tcaactttta
     8821 tggtctgatg gccgccaatc cgttcaaatt cccgttcctg tagaatgcgt agtaatttgg
     8881 cttgtagtac cagcggcatt tcgccaattt cgtcgaggag cagagtacct tcgttggctc
     8941 gttcaaataa tccctgacgc aaggtttgtg caccagtaaa tgcacctttt tcatgaccaa
     9001 acagttcact ttcgagcaac gattccggca gcgccgcgca gttgacttta atgaacggcc
     9061 cctttgcccg ccgcgaattg tagtgaatcg ctctggcaat caactctttc ccggtgccgc
     9121 tttcaccgct aatcaagacg ctggcctgag aaagggcaat tttggcggtg tctttgcaga
     9181 tgtccatcat cgccgggctg ttggtgagaa tgtgccccca ttgccagctg gcgctcagtg
     9241 cctggtgcag atgacggatc tcttttttca ttgactggag ttgtaaagcg cgctgaacga
     9301 ttaaattcaa ctcatcgaga tcaaacggtt taataacata gtcgaaggct ccgcagcgta
     9361 gcgcttcgac ggcggtttcc acttccgcat aggccgtcat cagaataacg ggtgtccggg
     9421 tctcatggct gcgcatctcc tttagtgcct tgatgccgtc catctctggc atgcggatat
     9481 ccatcaacac cacatcaggg tgaatatcgg caaacaggtg taatgctgtg cgtccgttgt
     9541 tcgcacaatg tgtttcgaat ccttgtagtg caaaagcggt gctcagcata cggcgaacat
     9601 tatcttcatc atccacaata aggatgcgat taatagcagt catacagtct gatttccctg
     9661 cgggttgatc ggtaaaataa gcgtgaaggt tgcgccgtag cccggcaaac tggcgacgcg
     9721 aatatcaccc tgatgggcat taatgatgcg ttgacttaac gccagaccaa gcccggttcc
     9781 tgaggctttg gtggtgaaaa agggatcgaa gatctttttt tgcagcgaga gatcaatgcc
     9841 acagccgttg tcctctatcg aaatggcctg ttgtgagtcg ctgtattgcc aggtttgaat
     9901 gcgaattttc cctcgtgcgc tgatagcctg gacggcattg atcaggatat tcagtagtac
     9961 ctgtttgagc agttcacgat cggcgttaat cgggcttaat tcattatcca gttcgcttat
    10021 gaagtcgacc cgcgcttgta cgccggcggt ttgtaccagt accagagttt cttcaaccaa
    10081 tgcattgagg ctgacttgtt gccattgact gtggcgtgga cgtgaaaatt cgagcaattg
    10141 ctgaataact ttgttaattg aatcgatttc tttgagtact acggacagat attcctgatg
    10201 tattgggtca ctggtttgtt ggcgcaagat ctgtacataa ccacgaatag ccgttaacgg
    10261 attacgtact tcatgcgcga cgccagccat cagctcaccc agtgtggcga ggcgttctgc
    10321 ttgcgccatg cggcgctggg tttctttgcg ggcagttaaa tcagagaaaa tcaccaaagc
    10381 acctatcatt tcaccgtgcg tgttatgaat acgactggta gtgacactga gttcaatggt
    10441 gcggtcacga cctggaaaac tgatctccag cgccacatgt tcggtgccat gttccagcgt
    10501 atccagtact ggactgtaga actgagtatt gtcgaacaac atggagtaag gctgccctac
    10561 cagttcatgg cgttgatagc cagtgataac ttctgctgct gggttcatgg tggttacatc
    10621 accctggcgg tcaatggcaa tgacgccatc ggcagcgttt tcaataatca gatcgttaag
    10681 tgtccgcgtt tcacgcagtg cctgggcgag gttattaaca ctctgactga tttgccccat
    10741 ttcaccgggc aattgtggta atcgagtggg aatattttgt gccagagtcg agaggccatc
    10801 ggtgatgata tcaatattgg cgctcaggcg acgggagaaa aggacaatca atagcaggct
    10861 tatcagcaaa tcggcggtga gcacaatgat aatcctcaca tccattttcc aggcctggcg
    10921 gcgaatatct tcggttaatt cattggccca gatatagccg aggatttcac cattacgctc
    10981 aatggggagc attgaattca aaatatcgcc gcgcacctgc ctgcctgaat aaaccaaagg
    11041 ggtatttgta cgcatgactt cgcgaccagg gtgatctgcg gcaatggtaa cgccgacatt
    11101 attctgatat agcgctgaag gcgcgtaggt gattatcgca tccagcattt tgttgtaata
    11161 accagcacct atgccaggga aggcgtgagt gatattttcg gtaatggggg caagttctgc
    11221 atttaatgcg cggatacgct cctcacgtgg taagtcgatg tagagatcat agcgatcgcc
    11281 tagtgcctga ttaagcaggt tgaccacggc agaaagtttt ttctcttttt cagataagac
    11341 tgctgaacgt ccttccgttt ctacgatata accaatagta agcgttggga caatgaccat
    11401 caggattgcc atcaggatca tttgattgcg taagcggcgt ggataaatcc acttcatata
    11461 atgcatgctg aaatccacta gtcttgtccg gtatatgacg attatcagag gttaaggtga
    11521 tgatttctcg gcggtgtatc atattccaga gaagagagaa cattgcggta acacgctttt
    11581 accgctacct taaccacact ccatcggtca cctgaggcgg agcttcgccc ctttgaaata
    11641 ccttgcttct tttcgtacaa ccctgaaagc ctcgcgctac atgttcagag cattggcgtt
    11701 agtgctctgg ctgttgattg ctttttcatc cgttttttac atcgttaatg cgttacatca
    11761 gcgagaatcg gaaattcgtc aggaatttaa tctgagttcc gatcaggctc agcgctttat
    11821 tcaacgcacc tctgatgtga tgaaagagct gaagtacatc gccgaaaatc gcttatcggc
    11881 agaaaacggt gtgctttccc cgcgtggacg agaaacgcag gcggatgtgc ctgcgtttga
    11941 accgctgttt gccgactccg attgttccgc aatgagtaac acctggcgag gttctctgga
    12001 gtcattggcg tggtttatgc gctactggcg cgataatttt tctgcggctt acgatctaaa
    12061 ccgggtattt ttaatcggca gcgataacct ctgcatggcc aatttcggtc tgcgtgatat
    12121 gccagtggaa cgcgataccg cgttgaaagc tttgcatgaa cgcatcaata aatatcgaaa
    12181 tgcaccacaa gatgatagcg gcagtaacct ctactggatc agcgaaggtc cgcgccctgg
    12241 cgtcgggtat ttttacgcgt tgacgccagt ttatctggcg aaccggttgc aggcgctttt
    12301 gggtgtcgag cagaccatcc ggatggagaa ctttttctta ccgggtacgt tgccgatggg
    12361 ggttaccatt cttgatgaaa atggtcatac cctgatttcg cttaccggac cagaaagtaa
    12421 aattaagggc gatcctcgct ggatgcagga acgctcctgg tttggctata cggaagggtt
    12481 ccgggagctg gtgctgaaga aaaatctgcc accctcatcg ctaagcatcg tgtattcggt
    12541 gccggttgat aaggtgctgg aacgcattcg catgttgatc cttaacgcaa ttttgctgaa
    12601 tgtgcttgcc ggagctgcat tgtttactct cgcacggatg tacgagcgac gtattttcat
    12661 tccggcggaa agcgacgccc tgcgactgga agaacatgag cagttcaatc gcaagattgt
    12721 cgcctccgcg ccagtgggta tctgcatttt gcgtaccgct gatggcgtca atattttaag
    12781 taacgaactg gcgcatacct atctcaatat gcttacgcat gaggaccgcc aacgactgac
    12841 gcaaattatc tgtgggcagc aggtcaattt tgttgatgtc ctgaccagca acaataccaa
    12901 tctgcaaatc agcttcgtcc attcgcgcta tcgtaatgaa aacgtggcca tttgtgtgct
    12961 ggtggatgtt tcttcgcgcg tgaagatgga agagtcgttg caggagatgg cacaagcagc
    13021 ggaacaggcg agccagtcaa aatcgatgtt ccttgccacc gtcagtcatg agctgcgaac
    13081 gccgctgtat ggcattatcg gtaacctgga tctgttgcaa accaaagagt taccgaaagg
    13141 cgtcgatcgg ctggtgacgg caatgaacaa ctcttccagc ctgttgttga aaattatcag
    13201 cgatattctc gatttctcga agattgaatc ggaacagttg aagatcgaac cgcgtgagtt
    13261 ttcaccgcgt gaagtgatga accacatcac cgccaactat ttaccgctgg tggtacgcaa
    13321 gcagttaggc ttgtactgct ttattgaacc ggatgtgcca gtggccttaa atggcgaccc
    13381 gatgcgttta cagcaggtca tctccaacct gttgagtaac gccataaaat tcaccgatac
    13441 cggctgtata gttttgcatg tccgcgctga tggcgattat ctctctatcc gtgttcgcga
    13501 taccggcgtg gggattccgg cgaaagaagt ggtgcgcttg tttgatccct tcttccaggt
    13561 cggaacgggc gtacagcgaa acttccaggg gaccggtctg ggtctggcga tttgtgaaaa
    13621 actgatcagc atgatggacg gcgatatctc ggtagattca gaaccgggaa tgggcagcca
    13681 gtttaccgtg cgtattccgt tgtacggcgc tcagtacccg cagaaaaaag gcgtggaagg
    13741 gttgagtggt aaacgctgct ggctggcggt ccgcaatgcg tcgctctgtc agttcctgga
    13801 aaccagtttg cagcgcagcg gcatcgtcgt tacaacatac gaagggcagg aaccgactcc
    13861 cgaagatgtg ttaattactg acgaggtagt gagtaaaaaa tggcagggta gagcggtagt
    13921 gaccttctgt cgtcgccata ttggtattcc gctggagaaa gcgccagggg agtgggtaca
    13981 cagtgtggct gccccgcatg agctaccggc attgttggcg cgtatttatt tgatcgagat
    14041 ggagagcgac gatcctgcta acgctctgcc gtcgacggac aaagcggtca gcgataatga
    14101 cgatatgatg attctggtcg tggatgatca tccgattaac cgacgtctgc tggcagatca
    14161 gttgggatcg ttgggctatc aatgtaaaac cgcgaatgat ggtgtcgatg cgcttaatgt
    14221 acttagcaag aatcatattg atatcgtgct tagcgacgtc aacatgccaa atatggatgg
    14281 ttaccgcttg acgcaacgca ttcgtcagtt gggactgacg ttgccggtaa tcggagtaac
    14341 tgctaatgcg ttggctgaag agaagcagcg gtgtctggag tccggtatgg acagctgcct
    14401 gtcgaagccg gtaacgctgg atgtgataaa acagacgctg acggtatatg ccgagagggt
    14461 caggaaatcg cgggaatcgt aggacggata aggtgtttat gccgcatctg gcattcagtg
    14521 caaatgccag atgcgacgct gacgcgtctt atctggccta ctttgatgta atctgttgaa
    14581 ataatgggaa tcgtaggacg cataaggcgt ttacgccgca ttcggcaatc ggtgcaaatg
    14641 ccagataaga cactaacgcg tcttatctgg cctacaggtg attagtcttt atctgccgga
    14701 cttaacgtca ctgaagagag ataattcagc agggcgatat cgttctcgac acccagcttc
    14761 atcatcgcag atttcttctg gctactgatg gttttaatac tgcggttcag ctttttagcg
    14821 atctcggtca ccaggaagcc ttccgcaaac aggcgcagaa cttcactctc ttttggcgag
    14881 agacgcttgt caccgtaacc accagcactg attttttcca acaggcgaga aacgctttcc
    14941 ggggtaaatt tcttcccttt ctgcagcgcg gcgagagctt tcggcagatc ggtcggtgca
    15001 ccttgtttca gcacgatccc ttcgatatcc agatccaata ccgcactaag aatcgccggg
    15061 ttgttgttca tagtcagaac aatgatcgac aggcttggga aatggcgctt gatgtacttg
    15121 attaaggtaa tgccatcgcc gtacttatcg ccaggcatgg agagatcggt aatcaacaca
    15181 tgcgcatcca gtttcggcag gttgttgatc agtgctgtag agtcttcaaa ttcgccgaca
    15241 acattcaccc actcaatttg ctcaagtgat ttgcgaatac cgaacaagac tatcggatgg
    15301 tcatcggcaa taattacgtt catattgttc atgtaatagg ctaccttgct acagcaagct
    15361 cttgacataa ctgtcaatgt cgctgatgta tttttctatt cctggaacat ccttctcacg
    15421 aatcagatgt tccagcgttt cacataactg cttgccgggt accagattta gcatggcaaa
    15481 tacgccttta agacgatgag ccgtttgggc taacgcagca aagtcactgg ttgctgcttc
    15541 agtatacagc ctcttaacat catccggtac tgtgtctaca aagagcgcat aatagccgct
    15601 ggcatggagt tgcgcatttt catctccgcc cagaggcgat tctgtcacct cttcctgcgc
    15661 cagttgcact tcaattaatt gtaagaccgc ttcctgcata gcgttgctca tattgaagtt
    15721 gacgcacaat tgaccaggcc caatttcccg tacgccagac tcatcatcgc ttaaaagcaa
    15781 gccagaggca gtaagattag acggattatc cgttaaaaag atatcataat cttgactaat
    15841 taatctttca tcgggtgtga tacaggttgc accccaattt tctaactggc gagtgacaat
    15901 attccgaatt tctgccgaag taacatccac cattacgcag acatcatcca gtaaacgctc
    15961 ttcttcctct tcaacttccg ggccagcggc gagcattttg atatgcacag agtagcgtgt
    16021 accaagccca tcccgcgttt tgatgtttaa atgaccgccc agtttacgtg ccagttgatc
    16081 gctcagccag aatgccagcg ggtctgcctt tccataacga tcgttttggg tctggttgat
    16141 aaacgggaag tgcaaattat ccatttcatg aatacttacg ccttctccgg tgtccagaat
    16201 gcggaatgtc aggcggtctt cagaggactc atcctgatca acctcaaggg tgatttttcc
    16261 caattgcgtt gaggtcacgg catattgcat cagtagcagc aaaatacgtc gtaaggcatc
    16321 gcgatcgccg cggcgcatat cgtgtgcttt cagatgattg ttaatcagca gttgcagacc
    16381 tttacgcttg atggcaggca acactgaagg cacaacttca tcaattaaat cctgcacgga
    16441 gaacagcacc gtctcacttt tccagctatc gtccgcaagc atgttcgcta actgtatttc
    16501 atcgaccagc cgcaccagca catctgcctg attcgccagt tgtttgcttt ccggggcgtt
    16561 gagtttagcc gcgctctccg ccagggactg tgcaggttct ttcagcgcat cgccaatgtt
    16621 tttcataaag gtcatccggc cctgctggtt tttctcatac agacgctgcg cctgcttgag
    16681 tttcttgttt accagcactt cgcgatcctg atcgcgaata atgaaaattt gtgtgcgcgg
    16741 cgcgacctgg ctgcggaaca tgcggatctc atacagctcg ttattgatcg tcgcctgaat
    16801 aatcccctga tgctgttccg ccatggtggt gatgttttgc agattcaaat gcggcagcaa
    16861 atgatcggca attttgttac ttatgacagt gcggttcgat tcctgatcgt gaaccagcag
    16921 gccgagcggc agcagtgaga ctatcttttc attgattgcc cgtaaaatgc gcaattcgtt
    16981 attgaccgcc gtgttgggta gactttctgt actgcgactg gagaaatggc ggaatgtggt
    17041 atagccaaat aacgccagcg ccagcaaacc gatgttcagc agcagtggca gcagaatgtt
    17101 ttgcaacgta tccagcaata aggtgccata aggaacctgc cagaccaggc gcatatcggt
    17161 agagttgagt gccgaggaga tttcaatctt cgtactgtta aagtggatac tgacgctatc
    17221 cgttccttct ttctcattat cattgtctcc cgttgccgtc gtgtctggct caaggcggaa
    17281 actgtccagc ggcatacccg gtgggatcaa atcattaatc ggcagatcaa aagccacgac
    17341 cgttgccaga tgtcccggct ggttgaacgt ggtacgcaag gtaaagtaat gaccgttctg
    17401 ccaggccagg cggcgcatgt tagaaaaact ttcgcgttca tcgagggcgt tggcctgttg
    17461 caacatttct gcacgacgtg aatcaacaat gtcactgacg gtcgattctt taaatccgga
    17521 ggtgagatct ttgaggggca gggttgagat cagtaccaga ctgttatcct gaccattcag
    17581 gtaatacatc gaccacggta cattttctgc accccacaat gtatccagat aagtggacat
    17641 ccgctgagtc atctcaagcg ttgagttgtc gtgagagcca aagattaacg cttccgtttt
    17701 gcggcgtggt ttctccagat agtagacatc ctgtttcagg cgcgtctctt gtaaaccttc
    17761 gccggaggag ggggaggtcg tcgcggcaat gttgtcgtag atctgccagg tcacgtaacg
    17821 ccaggtatcg acgcgctttt ggattgcgtg ggtaatgtcg acaatctggt aacttttatc
    17881 tttcagccag gcgttaacgg cgctttgtac cattaccccc atcgtcacca gtaacacaat
    17941 gatcaacagt aaaaagaagc gggtaatgct ccccggtagg agtgaaaagc gggtcgtggc
    18001 cgttgtctct ttctgacgca ttcgtgttta tgacctgtta aaacttcgcg aagtgatagg
    18061 gcagtataaa gggtacagtg tgaaattcca gactcttacc tgattgatga ttctctattc
    18121 tcccgtgata tttatcatcc tgcattgggg gaaatagatg tacaagcgcc attttgctgt
    18181 acaaataacc tacaaaaagc ccaacgaaat taattgcaaa taaacgaaga ttgctggaaa
    18241 ttatgctgat gttaattatt tgtgaaatag ttgacaagag ttatagtttt tctgtggtag
    18301 cacagaataa tgaaagtgtg taaagaaggg taaaaaaaac cggatgcgag gcatccggtt
    18361 gaaatagggg taaacagaca ttcagaaatg aatgacggta ataaataaag ttaatgatga
    18421 tagcgggaga tattctagtt gcgagtgaag gttttgtttt gacattcagt gctgtcaaat
    18481 acttaagaat aagttattga ttttaacctt gaattattat tgct
//
LOCUS       DAJVQG010000079        18164 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJVQG010000079 DAJVQG010000000
VERSION     DAJVQG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18164
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.079"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..1044
                     /locus_tag="OQM83_002414"
     CDS             <1..1044
                     /locus_tag="OQM83_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1520659.1"
                     /translation="RNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDAVTQAIEANHYI
                     YKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRANQLETIKEKLGE
                     ILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRISQNPVTPTRAP
                     KIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITNMRFSDIYDDRL
                     HVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGIRKNSPDGSIHP
                     DGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQRLLGHTSEKTTK
                     MYLDEREKTYLLL"
     gene            complement(1437..1778)
                     /locus_tag="OQM83_002415"
     CDS             complement(1437..1778)
                     /locus_tag="OQM83_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCS1520660.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1791..2663)
                     /gene="copD"
                     /locus_tag="OQM83_002416"
     CDS             complement(1791..2663)
                     /gene="copD"
                     /locus_tag="OQM83_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HCS1520661.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2667..3041)
                     /gene="yobA"
                     /locus_tag="OQM83_002417"
     CDS             complement(2667..3041)
                     /gene="yobA"
                     /locus_tag="OQM83_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCS1520662.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3180..3410
                     /gene="holE"
                     /locus_tag="OQM83_002418"
     CDS             3180..3410
                     /gene="holE"
                     /locus_tag="OQM83_002418"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCS1520663.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3512..4168
                     /locus_tag="OQM83_002419"
     CDS             3512..4168
                     /locus_tag="OQM83_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCS1520664.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4192..4854
                     /gene="exoX"
                     /locus_tag="OQM83_002420"
     CDS             4192..4854
                     /gene="exoX"
                     /locus_tag="OQM83_002420"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCS1520665.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4851..6911)
                     /gene="ptrB"
                     /locus_tag="OQM83_002421"
     CDS             complement(4851..6911)
                     /gene="ptrB"
                     /locus_tag="OQM83_002421"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCS1520666.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7120..7779)
                     /locus_tag="OQM83_002422"
     CDS             complement(7120..7779)
                     /locus_tag="OQM83_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCS1520667.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8106..8462)
                     /locus_tag="OQM83_002423"
     CDS             complement(8106..8462)
                     /locus_tag="OQM83_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCS1520668.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8529..8819)
                     /gene="yebG"
                     /locus_tag="OQM83_002424"
     CDS             complement(8529..8819)
                     /gene="yebG"
                     /locus_tag="OQM83_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HCS1520669.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            8953..10131
                     /gene="purT"
                     /locus_tag="OQM83_002425"
     CDS             8953..10131
                     /gene="purT"
                     /locus_tag="OQM83_002425"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCS1520670.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10187..10828)
                     /gene="kdgA"
                     /locus_tag="OQM83_002426"
                     /gene_synonym="eda"
     CDS             complement(10187..10828)
                     /gene="kdgA"
                     /locus_tag="OQM83_002426"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCS1520671.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10865..12676)
                     /gene="edd"
                     /locus_tag="OQM83_002427"
     CDS             complement(10865..12676)
                     /gene="edd"
                     /locus_tag="OQM83_002427"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCS1520672.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12911..14386)
                     /gene="zwf"
                     /locus_tag="OQM83_002428"
     CDS             complement(12911..14386)
                     /gene="zwf"
                     /locus_tag="OQM83_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCS1520673.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14724..15593
                     /gene="hexR"
                     /locus_tag="OQM83_002429"
                     /gene_synonym="yebK"
     CDS             14724..15593
                     /gene="hexR"
                     /locus_tag="OQM83_002429"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCS1520674.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15721..17163
                     /gene="pyk"
                     /locus_tag="OQM83_002430"
     CDS             15721..17163
                     /gene="pyk"
                     /locus_tag="OQM83_002430"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCS1520675.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEVVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17223..18065)
                     /locus_tag="OQM83_002431"
     CDS             complement(17223..18065)
                     /locus_tag="OQM83_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCS1520676.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         4650 a         4565 c         4382 g         4567 t
ORIGIN      
        1 agaaacttaa catatcgaag gagagacaaa gcatattact ggcgcaaccc tctgacgaaa
       61 gaagaattta cactaggtaa aatttcaaga agagatgcag taacgcaggc aattgaagca
      121 aatcattata tatacaaaaa ctactctccc gctgccttaa ttgaaaaact taaagggttc
      181 gactcattta ctatggcaga ctggattgaa cgttacaaaa cgattcttat aaggagaaaa
      241 gtgtccagaa atacttataa aattcgggca aatcaactgg agacaataaa agaaaaattg
      301 ggagagattt tactgacaga aataaccact cgccatattg ccgagtttct tgatttgtgg
      361 attgaaggag ggaaaaacac aatggcagga tcaatgcgtt ctgtgttgtc tgatatgttt
      421 cgcgaggcca ttgttgaagg acgtatatct caaaatccag tcacgccaac aagagcaccg
      481 aaaatagtag ttacaagaga acgactgaaa ctaaagatat acaactgcat cagggaggca
      541 gcagatcaac ttccggcatg gttcccatta gctatggatt tagcccttgt aacaggacaa
      601 cgtcgcgaag acataacgaa tatgcggttc agtgatattt atgatgatcg tctccacgtc
      661 aggcaaatta agacaggaat gatgattgct atccccctgt cactcagcct tcctgtcgct
      721 ggtctacggc ttggtacagt agttgaacga tgccgcctgg taagccgggg agattatcta
      781 atcagtgccg ggattagaaa aaacagccct gacggcagca ttcacccgga tggcctgaca
      841 aaaaaatttg tcgcagccag aaaattcaca ggtatccagt tcagtgaaaa cccaccaact
      901 tttcacgaga tcagaagcct ggctggacga ttgtacaaag aaacatgtgg agaagaattt
      961 gctcagcgtc tacttggcca cacatcggag aagacaacaa aaatgtatct tgatgagaga
     1021 gaaaaaacgt acttactgct ctgattttaa cgtaaatgga ttgttaaatg tattttggtt
     1081 gtgatataac caaaaagacc ggaatacaga aattcgagta aatttcgggg aatttcgggg
     1141 gagacatttg caactaattg atttcaaatg caattaaaaa aagaccgaat acgattcctg
     1201 tattcggtcc agggaaatgg ctcttgggag agagccgtgc gctaaaagtt ggcattaatg
     1261 caggcttagt tgccttgccc tttaagaata gatgacgacg ccaggttttc cagtttgcgt
     1321 gcaaaatggt caataaaaag cgcggtggtc atcagcttaa atgttaaaaa ccgcccgttc
     1381 tggtgaaaga actgaggcgg tttttttatt ggaaatcaaa aggctatttt aggtaattaa
     1441 cagagttttt cagctcgttc tataaacggt gccagactca ttttttcgcc gggattgtta
     1501 ggatcatcaa tctgaatcac cgaaatgggt tgggcattgg tcttcccact ggcaacttcc
     1561 ttttgtgcga tatcgtttaa aggatactgc acgagggtac tcggattaat aacatacaaa
     1621 gcattacccg gtcggcaagt cagcatcacc tcttcgcgat taaacgccca tttgtcttta
     1681 cccacttcaa aacgactgac ggtaatcacc tgcggtgcag ccagcgccgc tgcagaactg
     1741 gtgagtaaca gaaacgccag aatacttttt ttcatcattt tcgtaatcca tcaaaaaggt
     1801 tcgagagtcg caagcaggct gatgatcacc agcactaccg cacctatcgc ccattcgagt
     1861 ttagtcatcc acacaaacca gggtgccgca cgatcttcct cctgcctcat gcgcggtaca
     1921 agaacataac gattagccag cgcaatgacc accataatca tcaccaggat ggctttgagc
     1981 aataagagtt ggccccagta agtcgtaagc gtggggggaa atccggtaat caacaatgca
     2041 ttaagcacgc cgcttgccag tacgccgatc accgcaaaat gcccgcacca tgaaaaacac
     2101 atcagcgcct gaatagcctg gtgtcgccaa cggcctttga tgagttgcat acaccagagc
     2161 accggcagta atcccccaaa ccacgccgcc gcacaaatca ggtgaatcgc gtgattagtc
     2221 tgatggattt tcgccgttac cccttcattc agtgtcgcat gccccattcc cgccagcaga
     2281 ataaattgcg cggtagtgag cataaacagc aaacgtggca tattccgcgg ttgcataagg
     2341 gcgacgatca acgttacgag ggcgagaaca atttgccata accagacgcc accaaactgc
     2401 gtttgcaaaa cagcctgcca aatgtttggc gaaaatacat ctgtccatcc cgttcccatc
     2461 aacccgccct gaacggcgag cattgcagtg gcactgatca aactccagac ggcagcgtgc
     2521 tgttgtaaac gcagaaaacg cttcgccagc aagcgacgaa tcgtcaaggg ggccagccag
     2581 gcaccgtaca ttgcgaagcc aaaaaccagc atcagcgagg tgaaatggat aaatcgcagc
     2641 gcgatccagg taaacgccag catgatttat ttcacgctaa aggtgtagtg tcctttcgtt
     2701 ttatgcccgt ccaccgaaac aacatgccag tcaacggtgt aggttccggg tttcagcgaa
     2761 tcggccagtg gaacaatcag ttgcttttgg tcctgctcat ttcgcttcgc cggtaatgtt
     2821 ttaatatttt cgttttttgg ccccgtgatt tttgcaccac tgaatcctgt ttccacacct
     2881 tccgagaagt ttaaagtgat tgcctgcggt gcagctgtca cttgcgcgtt tgccgcagga
     2941 tactgatgcg ttaaatgcgc atgtgcccag acgaaaggtg tcaccaacga agtggtcagg
     3001 attgccagcg cgtagcgaag ggagcgtgca gttgaagcca tattatctat tcctttttgt
     3061 aataactttt ttacagagca taaccttgtc taatgtctga gtcgaggatc atcaattccg
     3121 gcttgccatc ctggctcact cttagtaact tttgcccgcg aatgatgagg agattaagaa
     3181 tgctgaagaa tctggctaaa ctggatcaaa cagaaatgga taaagtgaat gtcgatttgg
     3241 cggcggccgg ggtggcattt aaagaacgct acaatatgcc ggtgatcgct gaagcggttg
     3301 aacgtgaaca gcctgaacat ttgcgcagct ggtttcgcga gcggcttatt gcccaccgtt
     3361 tggcttcggt caatctgtca cgtttacctt acgagcccaa acttaaataa aacttataca
     3421 gggttacact ttcttacata acgcctgcta aattatgagt attttctaaa ccgcacccat
     3481 aatttgcagt cattttgaaa aggaagtcat tatgtcgttc tggaaagttg cagcagcgca
     3541 atatgagcct cgtaaaacct cactcaccga acaagtagct catcatctgg agtttgtgag
     3601 ggccgccgcc aggcaacaat gtcagctgtt agtttttcca tcgctttctt tactggggtg
     3661 tgattattcg cgacgtgccc ttcctgcccc acccgatctc tcactgttag acccgctttg
     3721 ttatgccgca acgacctggc gaatgaccat cattgccggc cttcccgttg aatataacga
     3781 tcgctttatt cgtggaattg cggtgttcgc accgtggcga aaaacgcctg ggatctacca
     3841 tcagagtcat ggtgcatgtc tgggccgtcg ttccagaacc atcacggtgg tcgatgaaca
     3901 accggaaggt atggatatgg acccgacctg ttcactgttt acgacagggc aatgtctggg
     3961 agaacccgac ctgctggcgt cggcccgtcg cttacaattt ttttcacatc agtactcgat
     4021 tgctgtgctg atggcaaatg cccgtggtaa cagtgcacta tgggatgaat atggtcgtct
     4081 catcgttcgc gccgatcgtg gttcattatt gttagtcggt cagcgttcat cacagggttg
     4141 gcaaggcgat atcattccat tacgctaggc tttttcggcc tggagtatgc catgttgcgc
     4201 attatcgata cagaaacctg cggtttgcag ggagggatcg ttgagattgc ctctgttgat
     4261 gtcattgacg gaaaaatcgt caaccccatg agccacctgg tgcgccccga tcgtcctatt
     4321 agtccacaag cgatggcgat tcatcgcatc accgaagcca tggtcgccga taaaccgtgg
     4381 attgaagatg tgatcccaca ctattacggc agtgaatggt atgtcgcgca caacgccagc
     4441 tttgaccgcc gcgtactgcc tgagatgccc ggtgagtgga tttgcaccat gaaactggcc
     4501 cgtcgcttgt ggccaggaat caagtacagc aatatggcgt tatataaaac acggaagctc
     4561 aatgtacaga cgccgccggg cctgcatcat caccgcgcgt tgtatgactg ttatatcacc
     4621 gccgcgttgc ttatcgatat tatgaacacc tccggctgga cggcagaaca gatggccgat
     4681 atcaccggac gtccgtcgtt gatgacgacc ttcacctttg gcaaataccg tggcaaagcg
     4741 gtttccgacg ttgccgaacg cgatccgggc tatctgcgct ggttatttaa taacctggac
     4801 agcatgagcc cggagctgcg tttaacactg aaacattatc tggaaaatac ttaatccgca
     4861 ggcgtagcgg gtaatgttcc ctgcgccagc gcgaccagaa aagcatattc catcgctacg
     4921 ccttcgtacg atttaaagcg gccagattta ccgccatggc ctgagtccat gtcggtacag
     4981 agcaataaaa gatggttatc ggttttcagc tcgcgcaatt tagcgaccca ttttgccggt
     5041 tcccaatatt gcacctgaga atcgtgtaaa ccggtcgtta ccagtaaatg cggataagcc
     5101 tgtgcggtga cgttgtcata cgggctgtag cttttcatgt actcgtaata ttgcggatcc
     5161 tgcgggttac cccactcttc aaactcacca gtggtaagag gaattgattc atcaagcatc
     5221 gttgttacaa catcaacaaa cggtacctgg gcgataacgc cgtggaataa ttccgggcgt
     5281 tgattaattg caacgcccat caacatgccc cccgcactcc cgcccatcgc ataacaaagc
     5341 gaaggagagc catagcccag ttttaacaat gcatcgcagg catcaagata atcattaaac
     5401 gtatttttct tcttcagaaa ttttccgtct tcgtaccatt gttgccccag ctcaccaccg
     5461 ccgcgaacat ggacaatggc gtagacaaag ccacgatcta acaaactcaa gcggctaaaa
     5521 ctgaaatcgg catcaatact tgcgccgtaa gaaccatagc catacaccag caacgggttg
     5581 tgtcctttgc gaaaatgttt gcgatggtag accaacgaaa ccggaacttc gacgccatca
     5641 cgggcgacta tccacaggtg ttcactgcgg taattcgccg catcaaaacc aggaacttcc
     5701 gtttgtttta atacacgacg ctcatcggta tccatatcca gttcaaacaa agtgtctggt
     5761 gtggtcatgg aagaataacc ataacgcaat cgcgcggttt caggttctgg attgtaggca
     5821 atccaggtca cataggctgg atcatcaaag gcaataccaa tgacttcccg ggtcttgcgg
     5881 ttaatttggc gcaaactggt taacccgcgc tgacgctctt caaccaccag ccagtcggta
     5941 aacagcgtaa acccttccag catgatgttt tcgcatggcg gaattaactc ttcccactgt
     6001 tgctcatcac gcatacgggt acggtataag ccaaagtttt tgccgtggcg gttggaacgc
     6061 agataaaaac gatgctggta gtgatcaagg ctgtattcgt gatctttgcg gcgcggcaga
     6121 aaaacaaacg gctcggcatc ggccatttcc gcgtccagca ggcgaacttc actggtggtg
     6181 gcgctggcca aatgaatgac tacatagtgc ttcgaggtcg ttttatgcag gctgacgtaa
     6241 taggtatcgt ctttttcttc gtagatcagt ttatcttgcg atgctggcgt accgatggcg
     6301 tgacgccaga cctgataagg cagcagcgtc accggatgct tgcgaacata gtagaaagtc
     6361 caggagtcat ttgcccagac aaagctgggt tcaacgttat ccagcagttc cgggtaccag
     6421 ttaccagttt ccagattacg aaaacgaatg ccgtactggc gtcgggaaag aaaatcttct
     6481 gccagcgcca taatggtgtt atcgggcgta atcgccattc cgcccatcga ataaaactca
     6541 ctatgagctg cgcgcttatt ggcatcgagc aatatttccc actcatccca ctcttcactg
     6601 aatgccgatt gacgctggta gatagcatat tcacagcctg gttcataaat atgccgatag
     6661 cggtagccat ttttgatgta gggcgcagaa acttctcgtt gcggaatgcg gtcgatgatt
     6721 tcctttaaga tgcgatcctg caaggcttgt tgtgaggcca tcacccgatg accgtaacta
     6781 ttttcttgtt gcaggtagtc caggacttct ggctgagaac gcgtatcatc ccgcagccag
     6841 tagtaattat cgatgcgcgt atcgccatga agcgtcatgg cgtggggaat gcgggcggct
     6901 tttggtagca tgttattgtt ctttctggtt gaaacatctt ataagggtgg caaaactcac
     6961 cggggatgca agcgaaacag gggagtcatt gcttagatga tgacaggtaa tggcacggat
     7021 atcgaatgtt atgcagacag agaaactcag cctgttcaaa tggctgtgcg gttctggata
     7081 gcccgaaata gtcaacttca ggctatccag agagcggaat tattccgcca aagtgcgttt
     7141 ttgctgttcg agatcgcgtt caatgccgtc acgaacatcc tgggggattt tcagcgcgtc
     7201 acccagtgca ttcaggtaac tgcgttccat aaaatggtca atatcaatag ccgcgcaact
     7261 caggaaatag atttccagcg cctcttcttc attgcggacc ccggtagcca ggcgttgtgg
     7321 atccagcggt tgttcgattg cctgctcaat gagtacacgc ccctgctctt ccacgccggc
     7381 ttcacgcaat tgctggtcga ttgccgcacg ttctttggca tcaatatgac catcactttt
     7441 agcggcaaag accagcgcaa ggatcaaacg ttctgtacgc tcatccagcg gcgtactttg
     7501 cgcgccaaac tgcggttcgt cctgatgcgc cgcgcgaatt ttatctttgt atttattcca
     7561 cagcaccgta cccgctaccg ctccgccgcc aaccagtaac gcgttggtgc catatttcgt
     7621 aagaagttta cgtgctgatt tatttgcgac cagcagccca gccagtccgc ctaatgcgcc
     7681 tggcactaac agtttgccca atccctgatc cgcagacgag gaggtagaag aactgctttg
     7741 cccaagcagg gattgcagtt gatttaacca gttagccata tttgctcctc aataaccatt
     7801 tttagtgcct gtagcatagc ggatgaggat gtcagaaaat gtatatcgag acaaaagatg
     7861 cgcaaataag cggggcggta ggccgttatt caaagaaatt cgcgccaggt aaggtattta
     7921 ctgtatatca ggtgataagg gtattttagg tgaaagtagt ttgtcgtttt gagtggcatt
     7981 gtttgatgct gattgcccga tgcgacgctg ttgcgtcttc tcaggcctac gattgtggtg
     8041 cgttctgggg cggacaaggc actcgcgccg catccaccaa cggtcaatcg aattatcaga
     8101 agccattaac gccgctgata ttccgccatt cccgctttgc agtccacgct gacctggtaa
     8161 tgaatatcgg cacttttacc acgcacggtt agcggtactg accatttatc atctttaccc
     8221 tgaatgtcct gcaaactgac ccaagccacg ggatcggcct gaccgacaat tttttgatca
     8281 tctgcccaac gcgccacgcg attttgttga taatcacgtt ttacgctcgc ggcaattccg
     8341 gcagcatcca gaccttcaca ctttgggaaa gtgaccgact tgctggtttc attattggca
     8401 gcgaaaactg atgcgcaggc agaaaccaac aacagcccta aaaacgcccc tctttttttc
     8461 atgtttttct ccatagcaca atgattcagg agaaagcatg gtacaaattg tcaggagcgc
     8521 aagttgcttc aggccgcgtg tgaggcatct tcctcttcgg attcagcacc gacgacctgt
     8581 ggggacggta atttgccggt tttcagaatg gtgctcagca catctttttg ttctgccagc
     8641 catagcgaaa gggcttcacg ttgctcgtct tccatttgca ctggagaatt tgtcagccag
     8701 gtgtcaagca gatccgccgt atcaagcatt ttgtcataag catcggcttc ctttttgctg
     8761 gtaaacgaca ttttctcttc gccctcacga atgactacgt atttaacttc aaccgccatt
     8821 tgcagcctct cataataact gtgattttat acagtatatt tcttttcggt tgagaaatca
     8881 acatcagcaa taaagacaca cgcaaacgtt ttcgtttata ctgcgcgcgg aattaatcag
     8941 gggatattcg ttatgacgtt attaggcact gcgctgcgtc cggcagcaac tcgcgtgatg
     9001 ttattaggct ccggtgaact gggtaaagaa gtggcaatcg agtgtcagcg tctcggcgta
     9061 gaggtgattg ccgtcgatcg ctatgccgac gcaccagcca tgcatgtcgc gcatcgctcc
     9121 catgtcatta atatgcttga tggtgatgca ttacgccgtg tggttgaact ggaaaaacca
     9181 cattatatcg tgccggagat cgaagctatt gccaccgata tgctgatcca acttgaagag
     9241 gaaggactga atgttgtccc ctgcgctcgc gcaacgaaat taacgatgaa tcgcgagggt
     9301 atccgtcgcc tggcggcaga agagctgcag ctgcccactt ccacttatcg ttttgccgat
     9361 agcgaaagcc ttttccgcga ggcggttgct gacattggct atccctgcat tgtaaaaccg
     9421 gtgatgagct cttccggcaa ggggcagacg tttattcgtt ctgcagagca acttgctcag
     9481 gcatggaagt acgctcagca aggcggtcgc gccggagcag gccgcgtaat tgttgaaggc
     9541 gtcgttaagt ttgacttcga aattaccttg ctaaccgtca gcgcggtgga tggcgtccat
     9601 ttctgtgcac cggtaggtca tcgccaggaa gatggcgact accgtgaatc ctggcaacca
     9661 cagcaaatga gcccgcttgc ccttgaacgt gcgcaggaga ttgcccgtaa agtggtgctg
     9721 gcactgggcg gttatggtct gtttggtgtc gagctgtttg tctgtggtga tgaggtgatt
     9781 ttcagtgagg tctcccctcg tccacatgat accgggatgg tgacgttaat ttctcaagat
     9841 ctttcagagt ttgccctgca tgtacgtgcc ttcctcggac ttccggttgg cgggatccgt
     9901 cagtatggtc ctgcagcttc tgccgttatt ctgccacaac tgaccagtca gaatgtcacg
     9961 tttgataatg tgcagaatgc cgtaggcgca gatttgcaga ttcgtttatt tggtaagccg
    10021 gaaattgatg gcagccgtcg tctgggtgtt gcgctggcga ccgctgagag tgttgttgac
    10081 gccattgaac gcgcgaagca cgccgccgga caggtaaaag tacagggtta aacccggcaa
    10141 aaaaacgcta caaaaatgcc cgatcgtaga tcgggcgttt tgacttttac agcttagcgc
    10201 cttctacagc ttcacgcgcc agcttagtaa tgcggtcgta atcgcccgct tccagcgcat
    10261 ctgccggaac cagccaggaa ccaccgatgc acagcacgct tttcagcgcc aggtagtcac
    10321 ggtagttagc cggagaaata ccacccgtcg ggcagaaacg gacctgggag aacggacccg
    10381 cgatcgcctg cagggctttc acgccgccgt tagcttcagc cgggaagaat ttgaactctt
    10441 tcaaaccgta gtccataccc agcatcagtt cggaaacagt gctgatcccc ggaattagag
    10501 gaatagtccc ttcggtagca gctttcagca gcggctcggt cagacccggg ctaattgcga
    10561 actgtgcacc cgcttcagtg acttctgcca gctgctgtgg attcagcacc gtaccggcac
    10621 ccacaatcgc ttcaggcact tctttggcga tagcacggat agcgtcaact gcacactcgg
    10681 tacgcagagt cacttccaga acgcgcaccc caccagcaac caacgctttt gccatcggca
    10741 ctgcgtgttc cagttttttt accacgataa ccggtacaac cgggccggtg gtcaggattg
    10801 attctgcact tgttttccag tttttcatca gagttttctc tcgcctgatt acaaatttgt
    10861 cgtcttaaaa agtgatacag gttgcgccct gttcggcacc ggacagtttt tcacgcaagg
    10921 cgctgaataa ttcacgtcct gtccccacgc gtgacgcgct caggtcagga atgtgcggtt
    10981 cgcgagcagc cagttccgct tcgtctacca gcagcgtcag ttcgcctgtc tgtccattca
    11041 cacgaatgat gtccccgtcg cgcacttttg ccagcagccc gccatcgtag gcttctggtg
    11101 ttacgtggat agctgacggc actttacctg aagcgccgga gagtcgtcca tcggtaacta
    11161 acgcaatttt gaaacaccgg tccaataata caccaagtgg cggcatgagt ttatgtaatt
    11221 ctggcattcc gttcgctttt ggcccctgat gacggacaac aacgacacaa tcgcggtcca
    11281 gcaaacccgc ttcaaaggcc ggcataacgt catgctggct ttcaaaaaca accgctggcg
    11341 cttcaatcac ctggttctca accggcacgg cagaggtttt cataaccgca cggcccaggt
    11401 taccgcttaa cacttttgtc ccaccatgat gagagaaagg ttgttcgaag gaagcgatca
    11461 cattgctgtc gagtgatttt tccgcccctt cccgccagtc ctgttcacca ttattcagcc
    11521 atggttcaag ggtataacga gacagaccaa agcctgccac cgtattgaca tcttcatgca
    11581 gcaggcctgc tttgagcagt tcacgcacca gaaccggtac gccacctgcc gcctggaagt
    11641 ggttaatatc ggccggaccg ttcgggtaga gacgtgccat cagcggtaca acatcagaaa
    11701 ggtcagagaa gtcatcccag ttaatctgaa taccggccgc gcgcgccatc gccaccaggt
    11761 gcatggtgtg gttagtggaa ccaccggtcg ccagcagtgc aacgataccg ttcaccacca
    11821 ctttctcatc gatcatctta ccgatcggca tccattcatt accattaccg gtcatgcgtg
    11881 taacctgacg cgcagcggca gcggtcaaag catcgcgcag cggagaatcc ggatgaacaa
    11941 aagaagagcc tggcaactgc atccccataa attccaccac catctggttg gtgttggcag
    12001 taccgtagaa agtacatgtt cccggcgcat ggtaagacgc ggcttctgac tccagtaagg
    12061 ccatgcggtc cactttacct tcggcataaa gctggcgaat acgcactttt tctttatttg
    12121 gcaaaccgct tgccatcggt ccagacggca caaacaccgc aggcaaatga ccaagcgaca
    12181 gggctgccat cgtcagaccc gggacaatct tgtcgcacac accgaggaac agagcaccat
    12241 caaacatgtt atgggacagc cccaccgccg cagacatcgc tatcacttcg cggcttagca
    12301 gcgacaattc cattccatcc tgcccctggg tgacaccatc acacatcgcc ggaacaccgc
    12361 ccgcaacctg accaaccgca ttcgcttcat gcagggcttt acgaatgatt tctggatagt
    12421 gttcataagg ctggtgcgcg gagagcatgt cgttatagga ggtgatgatg gcgatattgt
    12481 tacgcaacat gcttttcaaa gaggctttgt cttctggctg gcaggcagcg aaaccgtgtg
    12541 ccaggttacc gcatgccaac tgcgaacgat gaacggtcga cgttttcgct tgttctatcc
    12601 gggcgagata agcagagcga gtctcgcgcg aacgttcaat gattcgattt gttacgcata
    12661 acaattgtgg attcataaag gctcctgaaa ttgagttgtc agagcaggat gattcacaac
    12721 gcgtttcatt cagaggattt atgagtgaaa cgcctgtaac cggagctcat agggcaaaaa
    12781 cgtttcagtc agtgtaataa aaaaagcctc gtgggtgaat ccgcacgagg cctgaaagtg
    12841 taaaaattgt tctacaatct gtgcaagatc atgttaccgg taaaataacc ataaaggata
    12901 agcgcagata ttactcaaac tcattccagg aacgaccatc acgggtaatc atcgccaccg
    12961 aggcaacggg tccccaggtt ccggcctgat acggtttcgg cgcatcattg tccatcgccc
    13021 acgcctcagt aatggagtct acccatttcc aggcttcttc cacttcatca cgacgtacaa
    13081 acagtgcctg aataccacgc atggtttcca gcagcagacg ttcataggca tccgccagat
    13141 gcgtctgatt aaaggtttct gaatagctca gatccagctt ggtgatttgc aggttatgtt
    13201 tgtggtcaag gccaggaact ttattcagta cctggatatc cacgccttca tcaggttgca
    13261 gacggatagt cagtttattc tgcggcagat cctgccacga ctctttaaac agattcagtt
    13321 caggtgtttt gaaatagacc acgacttcag aacatttggt cggcagacgt ttaccagtac
    13381 gcaggtagaa tggcacaccg gcccagcgcc agttatcaat gtcgacgcgg atcgccacga
    13441 aggtttctgt attgctgctc ttgttcgcgc cctcttcttc cagatatccc ggcacttttt
    13501 tgccctgggc gaagcccgca gtatattgcc cgcgtacggt tttttcgcgt acgttggagc
    13561 ggtcgatgcg gcgcagggac ttcagtactt tcactttttc atcgcggatg ctgtctgcgc
    13621 tcaggtcaga tggcggagac atcgcaatca tgcagagaat ttgtagcagg tggttctgaa
    13681 tcatgtcacg catctgaccg gctttatcaa aatagcccca gcgcccttcg atccccactt
    13741 cttctgccac ggtaatctca acatgatcaa tggtgcgatt gtcccagtta ttcacaaaca
    13801 gggagttagc aaaacgcagc gccaacaggt tcagcaccgt ttctttacca agatagtggt
    13861 cgatacggta aacctggcac tcctcgaagt attcgccaac ctgatcattg atttcctgcg
    13921 aggtcgccag cgacgtcccc agcggtttct ccatgactac gcgtgccggt ttagcattca
    13981 gttttgcctc gccaagccct ttgcaaattg cgccaaaagt gctgggcggc atggcaaagt
    14041 agttaatggt gatacgattt ttttgatcca gcatcgcgcc gagacggttg aatgcagcag
    14101 tgtcattgac atcgagatta caaaaatcca gacgtgcact cagggtgtcc cataaacctt
    14161 catcaatggt ttctttcatg aaagtttcga gcgcctcgcg gacaactttg gtatatgccg
    14221 ctttatccca gtcagcacgc cctacgccga taatccgggt gtccgggttg agctgaccgg
    14281 ctttttccag ttgatacagg gaaggcagca atttacgacg cgcaaggtcg cctttcgcgc
    14341 cgaaaatgac caggtcacag gcctgggctg tttgcgttac cgccatgtca ttctccttaa
    14401 gttaactaac ccggtactta agccagggta tacttgtaat tttcttacgg tgcactgtac
    14461 tgcttttacg agcttgcgaa aactgtaaac gcttatccac ccgtgcgatt acgggaaaag
    14521 cgcgcaaagt gcggcaaaac tgataaaaaa atcatcgttt tccattcggt aaaaatctga
    14581 cactgatcat gttatgaaaa aaaataacaa cttttttatc tgcttttgtc attaacacgg
    14641 cacacaggcg taatatctga caaaactgca tttcgatttc tttcagtgcg gaaatcgtca
    14701 ttacccgtga gtctctttac atcatgaata tgctggaaaa aatccagtct cagctggaac
    14761 atttgagcaa atcagagcgc aaagttgccg aggtcattct ggcttcgccc gataacgcga
    14821 tccattcgag tattgctgct atggcactgg aagccaatgt tagcgaaccg acggtgaatc
    14881 gtttctgtcg cagcatggac acgcgcggtt ttcctgattt taaacttcat ctggcacaga
    14941 gtctggcgaa tggcactccc tatgttaatc gcaatgtcaa tgaagatgac agcgttgaat
    15001 catacacagg gaaaatattt gagtccgcaa tggcaacgct tgatcatgtc cgtcattcac
    15061 tggataaatc tgccatcaac cgcgccgtcg acttgctcac tcaggcaaaa aaaatcgcct
    15121 ttttcggatt aggctcttca gccgccgttg cccacgatgc gatgaataag ttctttcgtt
    15181 ttaatgttcc ggtggtgtac tccgatgata tcgtgctgca acgcatgagt tgtatgaatt
    15241 gtagcgacgg agacgtggtg gtgctgattt ctcacactgg aagaacaaaa aatctggtcg
    15301 agctggcgca gctggctcgc gaaaacgacg caatggtgat tgccctcacc tctgcgggta
    15361 ccccgctcgc ccgggaagca acgctggcaa ttaccctcga cgtaccggaa gatactgaca
    15421 tttatatgcc catggtttct cgacttgcac agctgaccgt gatagatgtg ctggcgacag
    15481 gatttacttt gcgacgcggt gcaaaattca gagataactt gaagcgggtc aaggaagcgc
    15541 tgaaggaatc gcgttttgat aagcagttac ttaatttaag tgacgatcgc taaaaacgac
    15601 tgtcactgtc ctaatcttat acgacatccg aatgagatta atttatcgcc atcgcggcgt
    15661 tatttcattc ggatttcatg ttcaagcaac acctggttgt ttcagtcaac ggagtattac
    15721 atgtccagaa ggcttcgcag aacaaaaatc gttaccacgt taggcccagc aacagatcgc
    15781 gataataatc ttgaaaaagt tatcgcggcg ggtgccaacg ttgtacgtat gaacttttct
    15841 cacggctcgc ctgaagatca caaaatgcgc gcggataaag ttcgtgagat tgccgcaaaa
    15901 ctggggcgtc atgtggctat tctgggtgac ctccaggggc ccaaaatccg tgtatccacc
    15961 tttaaagaag gcaaagtttt cctcaatatt ggggataaat tcctgctcga cgccaacctg
    16021 ggtaaaggtg aaggcgacaa agaaaaagtc ggtatcgact acaaaggcct gcctgctgac
    16081 gtcgtgcctg gtgacatcct gctgctggac gatggtcgcg tccagttaaa agtactggaa
    16141 gttcagggca tgaaagtgtt caccgaagtc accgtcggtg gtcccctctc caacaataaa
    16201 ggtatcaaca aacttggcgg cggtttgtcg gctgaagcgc tgaccgaaaa agacaaagca
    16261 gacattaaga ctgcggcgtt gattggcgta gattacctgg ctgtctcctt cccacgctgt
    16321 ggcgaagatc tgaactatgc ccgtcgcctg gcacgcgatg caggatgtga tgcgaaaatt
    16381 gttgccaagg ttgaacgtgc ggaagtcgtt tgcagccagg atgcaatgga tgacatcatc
    16441 ctcgcctctg acgtggtaat ggttgcacgt ggcgacctcg gtgtggaaat tggcgacccg
    16501 gaactggtcg gcattcagaa agcgttgatc cgtcgtgcgc gtcagctaaa ccgagcggta
    16561 atcacggcga cccagatgat ggagtcaatg attactaacc cgatgccgac gcgtgcagaa
    16621 gtcatggacg tagcaaacgc cgttctggat ggtactgacg ctgtgatgct gtctgcagaa
    16681 actgccgcag ggcagtatcc gtcagaaact gttgcagcca tggcgcgcgt ttgcctgggt
    16741 gcggaaaaaa tcccgagcat caacgtttct aaacaccgtc tggacgttca gttcgacaat
    16801 gtggaagaag ctattgccat gtcagcaatg tacgcagcta accacctgaa aggcgttacg
    16861 gcgattatta ccatgaccga atcgggtcgt accgcgctga tgacctcccg tatcagctct
    16921 ggtctaccaa ttttcgccat gtcgcgccat gaacgtacgc tgaacctgac tgctctctat
    16981 cgtggcgtta cgccggtgca ctttgatagc gctaatgacg gcgtagcagc tgccagcgaa
    17041 gcggttaatc tgctgcgcga taaaggttac ttgatgtctg gtgacctggt gattgtcacc
    17101 cagggcgacg tgatgagtac cgtgggttct actaatacca cgcgtatttt aacggtagag
    17161 taatctcacc tgccccggcc gctgatggcc ggggtattta gcaacacata aattaggact
    17221 acttattcaa tccaatcata atcacagccc cctgtaaaat gaatacaggg atagcctgaa
    17281 tggagatcta aatagcttgc aagacgtcgc atacccgctt cctctgcaac actatgtcca
    17341 ataactgcca tcggtaagcc actatctttg gagtattgtg tctgcaccca tgttttaatt
    17401 gaatcgttaa tcgtaatata ataatcagcc tgatattcca tatattgtat tggatcacaa
    17461 aaacagcctg ccccaatgcc aacagttctg actactctgg ctttatctcc ataaaattga
    17521 atgccaggaa gattatatat tttcaaattt ttggcaaaac gtttggtaat gttaatggca
    17581 ctgtcagggt ctatagcata tacacagtac atctcatcag aagcaacaat atttgtaaca
    17641 tccagttcta actgctgagc caaagcctta gacattccga acgtaggctc tcgatccatg
    17701 acatcatggc aacgaatgat taccaaatta tttttatgca aaaactcctt tttttgctca
    17761 attattttta aataactgag ttcaccgcga gtataatcca ttatttttct ggcccatgaa
    17821 aactcaagat cgtcaccctc cagatcagca taagaataaa atgtaggctc atgtgtaata
    17881 atgatattga caccttcaaa aacagccttt cttaaaacat ccattgaggc aatccagcag
    17941 gtcccaattt tattaataag tgtcgcctca gcaccaaaaa taattttatc aacagttggt
    18001 tgcgaaacag caaccattga atttaaaaaa caattaagat ccgcggctgt tttaatatga
    18061 tgcattcaac atccttcaca ggtttcatca gcaataaaat aatttcaaca tttctgggaa
    18121 acgttttgcc catgcagact cagagtgggt atcacctccc cata
//
LOCUS       DAJVQG010000080        17871 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJVQG010000080 DAJVQG010000000
VERSION     DAJVQG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17871
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.080"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..304)
                     /locus_tag="OQM83_002432"
     CDS             complement(<1..304)
                     /locus_tag="OQM83_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCS1520677.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
     gene            complement(514..1032)
                     /locus_tag="OQM83_002433"
     CDS             complement(514..1032)
                     /locus_tag="OQM83_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCS1520678.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1729..2229
                     /gene="tssB"
                     /locus_tag="OQM83_002434"
     CDS             1729..2229
                     /gene="tssB"
                     /locus_tag="OQM83_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HCS1520679.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            2264..2488
                     /locus_tag="OQM83_002435"
     CDS             2264..2488
                     /locus_tag="OQM83_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1520680.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            2539..4014
                     /gene="tssC"
                     /locus_tag="OQM83_002436"
                     /pseudo
     CDS             2539..4014
                     /gene="tssC"
                     /locus_tag="OQM83_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            4021..4434
                     /gene="tssE"
                     /locus_tag="OQM83_002437"
     CDS             4021..4434
                     /gene="tssE"
                     /locus_tag="OQM83_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCS1520681.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            4438..6288
                     /gene="tssF"
                     /locus_tag="OQM83_002438"
     CDS             4438..6288
                     /gene="tssF"
                     /locus_tag="OQM83_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCS1520682.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            6252..7334
                     /gene="tssG"
                     /locus_tag="OQM83_002439"
     CDS             6252..7334
                     /gene="tssG"
                     /locus_tag="OQM83_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HCS1520683.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            7359..8639
                     /gene="tagH"
                     /locus_tag="OQM83_002440"
     CDS             7359..8639
                     /gene="tagH"
                     /locus_tag="OQM83_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HCS1520684.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            8636..9160
                     /gene="tssJ"
                     /locus_tag="OQM83_002441"
     CDS             8636..9160
                     /gene="tssJ"
                     /locus_tag="OQM83_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HCS1520685.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            9163..10492
                     /gene="tssK"
                     /locus_tag="OQM83_002442"
                     /pseudo
     CDS             9163..10492
                     /gene="tssK"
                     /locus_tag="OQM83_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            10497..11258
                     /gene="icmH"
                     /locus_tag="OQM83_002443"
     CDS             10497..11258
                     /gene="icmH"
                     /locus_tag="OQM83_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HCS1520686.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            11267..14068
                     /gene="tssH"
                     /locus_tag="OQM83_002444"
                     /pseudo
     CDS             11267..14068
                     /gene="tssH"
                     /locus_tag="OQM83_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
     gene            14065..14808
                     /gene="tagO"
                     /locus_tag="OQM83_002445"
     CDS             14065..14808
                     /gene="tagO"
                     /locus_tag="OQM83_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HCS1520687.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            14813..16225
                     /gene="tssA"
                     /locus_tag="OQM83_002446"
     CDS             14813..16225
                     /gene="tssA"
                     /locus_tag="OQM83_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HCS1520688.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDSRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIH
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            16244..>17871
                     /gene="tssM"
                     /locus_tag="OQM83_002447"
     CDS             16244..>17871
                     /gene="tssM"
                     /locus_tag="OQM83_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HCS1520689.1"
                     /translation="MFRLPTPRLLSGLKSALRPAMPRFKVSAFWLLILAWIFLLVWIW
                     WKGPMWTLYEEQWLKPLANRWLATAAWGIIALVWLTVRVMKRLQQLEKMQKQQREEAV
                     DPLSVELNAQQRYLDRWLLRLQRHLDNRRFLWQLPWYMVIGPAGSGKTTLLREGFPSD
                     IIYAPEGARGAEQRLYLTPHVGKQAVIFDIDGTLCAPADADILHRRLWEHALGWLKEK
                     RARQPLNGIILTLDLPDLLTADKRRREHLLQTLRSRLQDIRQHLHCQLPVYVVLTRLD
                     LLQGFAALFQSLNRQDRDAILGVTFTRRAHENDDWRTELNAFWQTWVDRMNLALPDLM
                     VAQTHTRASLFSFSRQMQGSREPLVSLLEGLLDGENMNVMLRGVYLTSSLQRGQMDDI
                     FTQSAARQYRLGNNPLASWPLVDTAPYFTRSLFPQALLAEPNLATESRAWLIRSRRRL
                     TVFSATGGVAALLLITGWHHYYNGNYQSGITVLKQAKAFMDVPPPQGEDDFGNLQLPL
                     LNPVRDATLAYGDWGDRSRLADMGLYQGRRIGPYV"
BASE COUNT         4160 a         4899 c         4883 g         3929 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 tcgtcaggta ggccattttg tccagtacct gcggaaattc aagggagaga aactgctggc
      181 tgaccagaga gagctcgagg gaaaagagcg aagagagtga ctggttcaga tgaaaggaga
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag cgtgaaacgt aatccggttg
      301 acatagcaac ctccggggtt aaggaaaaac agacagttcg ctgttaaagc ggctgtgaca
      361 gtacagtgtt acgcttgcgt tcaggcacgc taccgccgaa gtttccggca tccgccatct
      421 cccgtgaacg taacccctta ctgacaacct ttctttctac ggcgtaatac aaaaacagca
      481 tccggcctga tagccggatg ctgttggctt aacttatgct tccagtggtg cgcgccagtc
      541 gtcggcaccg gaggtgccag atttgacgtg ttcccattca attttgcggt aggccagtga
      601 aacctcgagc aactgggtga actcgcgttt cgccgggtcc tggcaatgcg gcatatgcag
      661 tttcatatcg acaatcgtcg aatcagtcag gcgggtagtg aaataatgct cctgtttgcc
      721 ctccacagag gtacgccacc agtgaagttc agtggttggc agcatttcgc cggaggccag
      781 tgcgttgtac atcagcggaa ccgctttgtt cagcgccacg gtaaaaatga aaggcttgtg
      841 agcacgctga cccgcaggct gaccggactg aggagtggtc gggacagtaa cgttatgcag
      901 gaattcctgc accagcatct cgtcttcgtg cccctgcaca tagatgttgc cgacagaatc
      961 agcggtaaat gcacctgcag taatatttcc ctgggtctga ccagtgatgg agatataaca
     1021 cggtgttggc atttgtaaac tccttgttaa cgtggttaat tactacttac aatgacctgc
     1081 cgttaatccc agacaggcag atgcagctca cttcacggaa ccgcatcatt tttcagtatt
     1141 ttttacatac aaaccgagct agaatttacg gttgtcgtaa aaactatttt taaaatcatt
     1201 aaatctctta ttcagcaata acttaataat atatatctgg ataaccatac ccaactgttc
     1261 aaagtccgta ttgcctccat aaaatggcca aagctgaatc atgaataaat attgtattta
     1321 tcctttctga attatcattt tgataatttt tgagatattc cttaaaaata cccacttaat
     1381 caatcacaaa acaaatatat aaacaagcaa tcattagatt agcttatttt taatgaaatt
     1441 attatatact tcaataagtt acacaaaaac tctatattta aattattgcc ataatacacc
     1501 tgaagcgaat tgcacaggtt cacacaatct atttaacgct tgcagaatac ccctctttac
     1561 gatcgataaa aagtcctttt cttatttatt ttgaagcgta aaatgcgggg tgatgaagga
     1621 gtggtgattt tattttcacc gccattaggg cggcattgcc gatatttttt tgcggtacaa
     1681 atatgccgtg ttcatggcct gtacgagata acatgacgga tcacttttat gagcaaaaaa
     1741 tttgaaggca gcgtcgcgcc ccgcgagcgt attaatatat cgtatgtccc aaaaacagat
     1801 ggccagacgg ctgaagtgga acttccgctt aacatgctcg ttgtcggcga taccggaaat
     1861 acgcaggaaa catccccact ggatgaacgt caggcagttt ccgttaacaa gcacaatttc
     1921 ggtgcagtga tggctgaagc agccattggc ctcaatttta ctgtccccgc cacgctgaag
     1981 ggaagcacaa cagacgatga actgaatgtg gcgctgaata tcaaatcact ggatgatttt
     2041 tcaccagata gcgtggcccg gcaggttccg gaagtgaata aattgctgga actgcgtgaa
     2101 gcactgactg cactgaaggg gccgatgggt aaccttccgg cattccgtac ccagctccag
     2161 gcattgctgg aaaatgaaga gtcccgcgag cagttgctca aagaaatcgg tctggtgagc
     2221 aataaataac caccactatt tcattaaaca gggttatcca ggcatgacgg cacacaaaaa
     2281 tatctccggg acataccacc tctctgttgc agacattctg caggtggttt accaggtatg
     2341 tttcagcccg tccgttgaaa tcaaccagga tggtgtggct gcgctgatta caacactgga
     2401 tcgccgtatc agtgatctat tggatgagat tatccatttt tgtgagtttc agcagagcgc
     2461 gtcgcactgg cagcgcgtac ttcactgatg gcgaaaagcc ggtttcagaa acacagctaa
     2521 aacagaagga ttttcagtat gtctttacag gaagaggaac tcgtttccag tcatgccggg
     2581 cagcctgagc aggcatcgtc gctgcttgat caaatcatgg cacagacccg tattcagccc
     2641 ggttcggaag gttatgacgt tgccctccag ggtgtgacag cctttattgc cagcatcctg
     2701 caatccactg catctgccga accggtcaat aaactggccg tggacagcat gattgcagat
     2761 attgatgaac gcatcagccg ccagatggat gtcatcatcc atgcgcctgc ctttcagcag
     2821 gtcgagtcgt tctggcgctc actgaaaacc atggtggatc gcgttgattt ccgtgaaaac
     2881 atcaaggtca acgtgcttca tgtcaccaag caggaacttc tggaagactt tgagtttgcg
     2941 ccagaaatta ttcagtccgg attttataag cacgtttatt cgtccggttt cggacagttt
     3001 ggcggtaaac ccatcgctgc cgtgctgggc gcttatgagt tcaaaaatac cgcgcctgac
     3061 atgaaactgc tgcaatacgt cagtgccgtg ggcgcaatgg cgcacgcacc gttcctgtcc
     3121 tccgtttccc cggagtttat ggggctgaac tcgtggaccg aactgcctaa tatcaaagat
     3181 ctttatgcca tctttgaagg tccggcctac accaaatggc gcgccctgcg tgactcggaa
     3241 gattcgcgct atttagggct tacggcaccc cgcttcctgc ttcgccagcc ctattcacca
     3301 acagataacc ctgttaagaa ctttaactat tacgaagatg tcagccagaa ccacgaagat
     3361 tatctgtggg gtaatacggc ctggatgctg gcatgcaata tcgcagacag ttttgccaaa
     3421 taccgctgat gtccaaacat tatcggtccg caaagcggcg gcgcagtgaa agatctgccg
     3481 atgcatctgt tcgaaacgat ggggcagatt caggccaaaa tcccaaccga ggtgctggtc
     3541 accgatcgcc gcgaatttga actggctgaa gagggtttta tcacccttac catgcgtaaa
     3601 gactctgata atgcagcctt tttctctgca aactcggtac aaaaaccgaa gcactttccg
     3661 ggaaaagatg cagaaaccaa ctataagctg ggtacgcagc ttccttatct cttcatcatc
     3721 aatcggttag cgcactacat caaggtgttg cagcgtgaac aactggggtc atggaaagaa
     3781 aggagcgact tagaaagaga gcttaatacc tggatccgtc agtatgttgc cgaccaggaa
     3841 aacccgcctg ctgacgtgcg cagccgcaaa cctctgcgcg ctgccaaagt tgaggttatg
     3901 gatgtagaag gcgaacccgg ctggtaccag gttgcgctaa gcgtgagacc tcattttaaa
     3961 tttatggggg caaattttga gctttccctg gttggccggt tagacaggga gtaacacacc
     4021 atgataccgg atcgggaaag aaccacaggt tctctttttg agcgtatgga agcctcttcg
     4081 gccagaaacc ggcagggagg cagtattcat tcgctcaggc aatccattcg ccagaaccta
     4141 cgaaacattc ttaatacccg gtccggtagt tgccggggtg cgcctgagct gggcattgat
     4201 gaaccggaag gggctgaaaa cttccgcgaa tccatgagtc gggccatcga gcaatgtata
     4261 gaacgctatg aaccccggat ctcacacgct gaagtgcagg cagtggtttc atccgcctcc
     4321 agcccgctgg atatgacgtt tcatattaca gcctgggtga catttaacga aacgcatgag
     4381 gtgcttgaat ttgatatggc accgaatggc agccagcatt accgggtgga ctgacaaatg
     4441 gaatttgaag aacgctattt ccgggaagaa ctcgattacc tgcgccagct tagcaagctg
     4501 ctggcaacgg aaaaacccca tctggcccgc ttcctggccg aaaaagatgc agatccggat
     4561 attgaacgcc tgctggaagg ggtggctttt cttaccggca atctccgcca gaaaattgag
     4621 gatgaattcc cagaactgac gcacgggctt attaagatgc tatggcctaa ttacctgcgt
     4681 ccggttccgg caatgaccct tattgaatat acgccggata tggataagtc ttctgtaccg
     4741 gtgttaatcc cccgtaatga gcagtttaca accaacgccg gggaaatcag agttgatgaa
     4801 gtgctgccct ctgatgctaa aaaggaggag ccgcctccct gtaccttcac cctctgccgg
     4861 gatatctggc tgctgcccgt tcgcctggag cagattgaaa accgcagtac gacccgtaat
     4921 ggtgttatca acatcacctt ttcggtcgca ccgggaacgg acttccgcac gctggatctg
     4981 aacaaacttc gcttctggct cggcaatgac gacaactata cccgtgacca gctttattta
     5041 tggttctgcg aatacttgca gggtgccgac ctgactgtgg gtgaacagca tattcgcctg
     5101 cctgagttta tgctaaaagc tgtcggtttt gagccgcagg atgccatgct gccctggccg
     5161 aaaaacgtcc acagcggcta ccggatcctt caggagtatt tctgttaccc cgatgcgttt
     5221 ctcttttttg atctttgtgg ttgtccggct ttgcctgacg gattgcaggc ggaattcttt
     5281 accctgcaac tgcgtttttc gcgccctttg cccgtggaca tccggctgcg ccgcgattcc
     5341 ctgcgcctgt attgcgcacc tgccattaat ttatttatcc accatgcaga agccatcacg
     5401 ctggacaacc ggcgggcaga ctatccgctg gttcccagcc gccattaccc acaacattac
     5461 gatgtatttt ccgttaacag tgtggtgagc caggtccagg atatgttcag gaaaaaagat
     5521 ctggggcgtc ctgtttcgac gcaggccgcg cgccagtggc cagcctttga aagtttcagc
     5581 catcagatag aatacagccg gaagcgggaa gtggtgtact ggcatcaccg gaccaaaaca
     5641 tccctgttcc atcgcggctt tgatcatacc cttgccttta tacatgctga tggcagttat
     5701 ccgtcagacg aatctctgct cagtaatgaa gtggtttcgg tatcgctgac ctgtaccaac
     5761 cgtgagcttc cgtcacaaat tcgttccggc gatatcaccg gcacaaccgg aaaaaatgcg
     5821 gctgttgctt catttcgcaa cattacccgc ccgacgcaac cactctggcc ggtcattgat
     5881 ggcagcctgc actggcccct actctccgcc atgaacctga attatctgtc attactggat
     5941 acggacgcgc tgaagcaggt catcgccaac tttgatcgcc acgcaatcca tcatccgcag
     6001 acggcacggc tgtcacaaca aaagctggat gccattgagc gtctggagac ccgccccgtt
     6061 gatcgcctgt ttacgggtat tcccgtccgg gggctggctt ccacgctgta tctgcacccg
     6121 gagccgtttg tctgtgaagg ggaaatgtat ctgctcggta cggtgctttc gcattttctg
     6181 tcgctgtacg ccagcgttaa ctcattccac atgctgaccg ttgtgaacac agaaagccag
     6241 gagacatgga aatggacgga aagaatcggg cagcatcctc ttatctgagt ccgggaaatc
     6301 ccccggcaga taaagagcag aatgatccgc tggcacaggt tttccataat gcctgctctt
     6361 acaatttttt tgcgatggcg gagctgttgc accgcctggc aaagggtgaa aaggggacgc
     6421 cagaattatc cctgcgcgac gatccggcac aggaaaccct gcgtttcagc gccgatgcca
     6481 gccttgcctt cccgtgcagt gatatcagtg cgctgaaacg ggatacgtca ggggcattca
     6541 ggatgaccac cacctttatg gggttacagg ggagccagtc gcctctcccc ggttattacc
     6601 tcgctcacct ggcctggaaa gcggtacatg aacaaagtcc ggtaggtgat tttctggata
     6661 tgttcagcca ccgcctgacc cagtttgtct ggcatatctg gcgtaagtac cgctatcaca
     6721 tcagttttcg taacggcggt gtggatgcgt tctcgcagcg catgtactcc cttgtcgggc
     6781 tgggccaccg ccagcttcgc gataagctgg cgattaatca cagcaaaatg ctggcgtatt
     6841 ccgggatcct ggcaaatccg ggacgatccc cggaaattat ctgtggcctg gtgtcccact
     6901 gctttgattt gtcagaagtg acactgcaaa actggcaacg tcgcaaggtg gatattgaac
     6961 cggatcagca aaatagtctg gggagctatt ctcttaaaaa tggcgaaaaa ctggcgggaa
     7021 gatcggtact gggcaacttt gttctgggta cacgagtgcc cgatctcagc ggaaaattcc
     7081 agctcagtat taccagcctg acccgaaaac agttcctctc ttttctgccg tccggcgaaa
     7141 acttcctgcc actgacgatg ttcgtgtcct tcatcctgcg cgatcagctt gcctgggact
     7201 tacatctggg gctggcgccg gagcaggtgg gcgcaatgcg cctgggcgat aacaaaagtg
     7261 cgctgctggg ctggaccagc ttcctcggca caccggaaga acgaccctca gtcaccatca
     7321 gggtacggtc gtaaattatg ataatcacgg agcagaatat gccggaagag aaactacaga
     7381 cgctttcatt gcaggtcatt aacggcagtg agctggaaag cgggcgggcg gcgcgctgtc
     7441 tgttcacaca gcagggaaat gtcggccatg gccccgaatg ccactggtcg gtacaggatc
     7501 gtcagcagag cattccggcc caggctttta ccgttatcct gcacgatggc acattttgtc
     7561 tgcgcccgca gacggcacaa ctgtggctga atcaggcaaa agtcacagca acatcagacc
     7621 taatacagtt gcgccagggc gatgagatcc agatcggacg gctgatggtg agggttcatc
     7681 tgaaccgggg agatattccc cattacgatg aggaaatggc cactcccgaa accatcgtta
     7741 ccaatcgcga tatgctcacg gataccctgc tatcaacgga gggtgcgcca cactatccgg
     7801 gaatgactca ccggcaccag cttgcagaca ccgtggtaaa cggtttttct gccgatccac
     7861 tccaggctct tcagtccgaa agcctgatta ccacgggcga tccgctttca ggcattgcgg
     7921 ctgtccggcc atcggcaccg ctgtccgatc cggcaagtaa tggggggatc aatactccgt
     7981 ttatggatct gccgcccatt tatgccagcc ccggcgatcg taatgacgac gtctctgcgg
     8041 cagaaatggc acaacgccac gttgcggtca cccccttact gcgcggtctt ggcggctcgc
     8101 ttaccatgag caattccgac gatgcggatg attttctgga ggaggccgga cgaacgttac
     8161 aggccgcaat aaaaggtctg ctcgatttgc agcagcagcg taacagcctc tcagacaaac
     8221 atttgcgccc gctggaagat aacccgctgc gcctgaacat ggattacgcc accgcgctcg
     8281 acgtaatgtt tgccgaaggt aaaagcccgg tacatctggc ggctcccgcc gccgtcagtg
     8341 aaagcctgcg caatgtccgc caccacgaag aagctaaccg ggcagcgatt gtggagtcgc
     8401 ttcgtgtcct gctggatgct ttctcaccac aaaatctgct gcgccgcttt gtgcagtacc
     8461 gccgcagcca tgaactgcgc cagccgctgg atgatgccgg agcatggcaa atgtacagcc
     8521 attattacga agaactggcc tccgatcgcc agcaggggtt tgagatgctg tttaacgagg
     8581 tctacgccca ggtctatgac cgggtgcttc gtgaaaaaca gcgggagccg gaagcatgaa
     8641 caggaaggcg tttctggcct gcgtgctgat gtgcgtcata ttaaccggct gcgaaacggc
     8701 gaaaaaaatc agtgaggtga tcaaaaaccc ggatatccag gtcgggagcc tgaaggagca
     8761 accttcggaa ataaccgtca ccctgctgac cgaaccggac accaatacca atgcggaagg
     8821 tgaatcagcc gccgtggatg tgcaactggt gtacctgact gacgactcaa aattgctggc
     8881 agccgactat gaccagatag ccagcacgcc gctaccggat gtgctgggta aaaactatat
     8941 cgaccaccag gatttcaacc tgttgccgga caccatcaaa accctgccgc cggtaaagct
     9001 ggatgagaaa acgcagttta ttggtgtcgt ggcctatttc tccgatgacc aggccactga
     9061 atggaaacaa atcgagacgg tggaaggaat cggccaccat taccgcctgc tggtgcatgt
     9121 acgtcaaagc agcatcgaaa tgaaaaaaga ggatgaataa ttatggcaac cacccgcaac
     9181 aaggtgatgt ggcaggaagg gatgctgatg cgtccacacc atttccagca gcagcagcgt
     9241 tacaacgact acctggataa ccagcgtttc cgggccatga atgatttatc ctggggattt
     9301 accgaactca ccctcaacaa tgaactgctg gcgcagggta agatcatgat tgacagcgcg
     9361 tcaggcacac tgcccgacgg caccgtcttt tctatccccg accaggacgc actgcccgat
     9421 ccgctgcacc cgcaaaattt tccggacgaa agaagccgca atatctacct cgctctgccg
     9481 gtcgccagtg atgtgagaaa tgaaatcagc gacggacggc gaatcgggcg ttaccggctg
     9541 aattatgccg atgtccggga tttgcattca gaagaaggtg acgcgcgaac attgacgctg
     9601 ggtcaactga cgccgcgcat tatgagcggt gcagaagata tgagcgccta tattacgctc
     9661 ccgctttgcc gtatcagcga tcgccgccga tggctccctg acgttggatg acgattttat
     9721 cccctcctgc cagaatattc aggtcagtaa gaaactgcgt gtttatctca aagaggtaca
     9781 gggggccatt ggcggacggg caagcgatct ggcaaaccgt attggctctc cggcgcagag
     9841 cggcatcgcg gatgtggcgg aatttatgat gttgcagtta cttaaccgta accagacccg
     9901 gtttacccat cacgcccgtc gatcccagct ccacccggaa gatttctacc ttgatcttgc
     9961 cgggttgctg ggtgaactga tgacctttac cgagccgtcg cgcctgccct gcccgcttga
    10021 tgtgtatgat catcatgacc tgaccaaaat atttaaaacg ctgttaccgg aagtcaaacg
    10081 ggcgctgcat accgtactgt cgccaagagc cgtcaatctg ccgctgcatc tgcgtgacgg
    10141 tatctggcag gccgatatcc atgacacaga actgctgcaa tctgccacct ttgtgctggc
    10201 cgtggcggca aacatgccgg tcgatcagat ccagcgccag tttatccagc agtcgaaaat
    10261 ttcctcgccg gaaaaaatcc gcaatatggt cagtgtgcag atccccggta ttccattgcg
    10321 tgcccttatg gtggctcccc gccagcttcc ttaccattcc gggttcagct atttcgaact
    10381 cgacaagagt ggccaggcct ggacagaaat ggctgccgcc ggggccgttg cactgcatgt
    10441 ttccggcagt ttcccggatc tgaacatgca actgtgggcg ataagagggt aagacgatgg
    10501 atgaggggtc actgtctctg ccaccattta ccggacacga cgaaaaaagc cagcgcaact
    10561 accatctcgc gcttcgtggc aacagcctga atccgatgat cgacgcggcc acgccgctac
    10621 tcggtatggt aatgcgcctt tcaaccatga acagccagac gatgccggag catctttttg
    10681 ctcaggtggt gacagatgtt caggccgtgg agcaactgct tcaggagcag ggctacgaac
    10741 cgggcgtgat tatctcgttt cgctacatcc tctgcacgtt tattgatgaa gcggcgctcg
    10801 gtaacggctg gtcaaacaag aacgagtgga tcaagcagtc attgctggtc catttccaca
    10861 acgaagcctg gggtggcgag aaagttttca tcctgctgga gcgcctgatc cgtgaaccca
    10921 aacgttacca ggatctgctg gaatttctct ggatctgttt ttctctggga tttcgcgggc
    10981 gctacaaagt ggcagcacag gatcaggggg aatttgagca gatctaccgc cgtctgtacc
    11041 acgttctgca taaactgcgc ggcgatgccc ctttcccgct gttacaccag gacaaaaaaa
    11101 cccagggcgg gcgttatcag ctcatcagtc gcctgactgt taaacatatt ttttgtggtg
    11161 gcgttgtcgt gctggcgttg ttttacctgt tctatctgtt gcggctggac agccagacgc
    11221 aggacatcct gcaccagcta aacaaattac ttcgataagg acgtttatga tccagattga
    11281 tcttcccacg ctggtaaaac ggctgaacct gttctcccgc caggcgctgg agatggccgc
    11341 ctctgaatgt atgagtcagc aggcagcgga aattaccgtc agccatgtgc tcattcagat
    11401 gctcgccatg ccacgcagtg acctgcgggt tattacccgc cagggcgata ttggcatgga
    11461 agagttgcgc caggcgctga cggtagagaa ctacacaacc gcccgttctg cggacagcta
    11521 cccggcgttt tccccgatgc tggttgagtg gcttaaagag ggctagctgc tggcgtcggc
    11581 tgagatgcag cacagcgaac tgcgcggcgg cgtgttgctg ctggccctgc tgcattcgcc
    11641 gctgcgttat ataccgcctg ctgccgcccg gctgttgacc ggcattaacc gtgaccgtct
    11701 gcaacaggac tttgtgcagt ggacacagga gtcggcggaa tcagtcgtgc cggatgcaga
    11761 cggtaaaggc gcaggcacaa tgacggacgc ctctgacacc ctgcttgccc gctatgccaa
    11821 aaacatgacc gcagacgccc gtaacggcag gcttgacccg gtactgtgcc gcgaccacga
    11881 aatcgacctg atgatcgaca ttctctgccg ccgccgtaaa aacaacccag tggtggtggg
    11941 cgaagcgggc gtgggcaaaa gcgcactgat tgaagggctg gcgctgcgca tcgtggcagg
    12001 ccaggtgccg gacaagctga aaaacaccga tatcatgacc cttgacttgg gcgcattgca
    12061 ggccggggcg tcggtgaagg gtgaattcga aaaacgtttc aaagggctga tggcggaggt
    12121 catttcctcc ccggtgccgg tcattctgtt tatcgacgaa gcacataccc tgattggcgc
    12181 gggcaaccag cagggcgggc tggatatctc caacctgctc aaaccggcgc tggcgcgcgg
    12241 cgagctgaaa accatcgccg ccaccacctg gagcgagtac aaaaaatact tcgaaaaaga
    12301 tgccgccctg tcgcgccgct tccagttggt gaaggtcagc gaacccaacg ctgccgaagc
    12361 caccattatt ctgcgcggtc tgtcggcggt ctatgaacag tctcacggcg tgctgattga
    12421 tgatgacgcc ttgcaggccg ctgcgacatt aagcgagcgt tatctctccg ggcgtcagtt
    12481 accggacaaa gcgattgatg tgctggatac cgcctgcgcc cgtgtggcca tcaacctgtc
    12541 gtcgccgccg aagcaaatct cggcgctgac cactctgagc caccagcagg aggcggaaat
    12601 tcgccagctt gagcgcgagc ttcgcatcgg actgcgtacc gacacatcac ggatgaccga
    12661 ggtgctggtg cagtatgatg aaacgctgac ggcgctggat gaactggaag cggcctggca
    12721 ccagcagcag acgctggtcc gggagattat cgcgctgcgc cagcagttac tgggcgtggc
    12781 agaggacgat gcggcgccgt tgccggacgc agataccgtg gaggatacgc agccagagtc
    12841 agagtcagag tcagagtcag agtcagagtc agagtcagag tcagagcagg ataataccgg
    12901 tgccgaaccg gctgatgagg ccgacagaga acaaccggaa gagaccgctg aaacagtttc
    12961 cccggtacag cggctggccc agctcactgc cgaactggac gccctgcata acgaccggtt
    13021 gctggtctcc ccgcacgtcg ataaaaaaca gattgcggcg gtgattgccg aatggaccgg
    13081 cgtgccgctc aaccgcctgt cgcagaatga aatgtcggtc atcaccgacc tgacgaaatg
    13141 gctgggtgac accatcaaag gccaggacct ggcgattgcc agcctgcata agcatctact
    13201 gaccgcacgc gccgacctgc gtcgtccggg acgcccgctc ggtgcgttcc tgctggctgg
    13261 ccccagcggt gtgggtaaaa ccgaaaccgt cctgcaactg gcagagctgc tctacggcgg
    13321 tcgccagtac ctgaccacca tcaatatgtc cgaattccag gagaaacaca ccgtctcgcg
    13381 gctgattggt tcgcctccgg gctacgttgg ctacggtgaa ggcggcgtac tgaccgaagc
    13441 gattcgccag aaaccctact cggtagtact gctcgatgaa gtggaaaaag cgcacccgga
    13501 tgtgctcaac ctgttctacc aggcgttcga taagggcgaa atggcagacg gtgaaggccg
    13561 cctgattgac tgcaaaaata tcgtcttctt cctgacgtcc aacctcggct accaggtaat
    13621 agtcgagcat gccgatgacc cggaaaccat gcaggaagta cagtatccgg tgctggccga
    13681 cttcttcaaa cctgccctgc tggcgcgtat ggaagtggtg ccgtatctgc cgctgtcgaa
    13741 agagacgctc gccaccatta tcgccggaaa actggcccgt ctggataacg tgctgcgcag
    13801 tcgctttggt gtagaagtgg tcattgaacc ggaagtgacg gacgaaatca tgagccgcgt
    13861 cacccgcgcg gaaaacggcg cgaggatgct ggaatcggtc atcgatggcg acatgctacc
    13921 gccgctctcg ctgctgctgt tgcagaaaat ggcggctaac acggcgattg cccggattcg
    13981 gttgtcggca gtggacggcg catttacggc agacgtggaa gatgctcaga acgacgagtc
    14041 cgtcacaaag gatgaaacgg ttttatgagc aggttgcgcg ttttccggca gacaggatgg
    14101 tttatcgcgg gcctgatgac gggcttaccc gccactgccg cgcccgcaga agcatcgtca
    14161 atggccggtg tggcggtcgc cgtcgccacc acgaccccgc cggatgcgac agccacgtta
    14221 caggccatgc agtcctgtcg gcgggagtcc gcggcgctgg agcggcttga ctgctatgac
    14281 cgcctgctgg caccgctgtc cccgtcaggg tttgacgggg cgctggtcaa agccggtttt
    14341 gtgggcgagg cctggacccg cgccacagag caggaaaaac gccgcgaggg caacaccaca
    14401 gaactgctgg tgacgcaggt gccgggggag cgcccgacgg tggtgataac caccccggct
    14461 atcggtcatg ttccgccacg cccggtgctg atgttcagtt gcgtggacaa tatcacccgg
    14521 atgcaggtgg cgctgatgca ccctctggat gtccatgata tcgccgtcac tctgaatgcc
    14581 gacaaccggg cattgcgcag ccactggttt gtgcgcgaga acggcaccct gctggagtcc
    14641 agccgggggt tatccgggat tgatgaaatt aagcaactgt tcggcgcaaa aacgctgacg
    14701 gtggataccg gcgcagacaa tgccgccgga aagctgacct ttaacattga cgggctggca
    14761 agggcgattg ctcccctgcg cgatgcctgc cactgggcgg gagaataaaa atatggcaat
    14821 ggacttacgc gatccgaatg tctggatatc gcacctgctg gaaaacctgc cggaagagaa
    14881 actggcatcg gcgctgaaag atgacaaccc gaactgggag tatatcgacg gcgaaatcgt
    14941 caagctggga tcgctggccc acagtcaact ggatatcccg gaactccagc gccgggggct
    15001 ggtcattctg gcttcggaga gtaaggattt tcgtctgctg gcgcatctgc taagaaccct
    15061 gcaacacgcc ggtgatcctc atctcgcatt gcgcctgctg gcgctgtatg tggagcatta
    15121 ctgggcggtg gctgcgccgc agaatatggc gcataaaaaa cgctttgcca gccaggtcat
    15181 taaacgcttt gaaacaggta ttgaagtttt ttcacagaac gccgccacag cgcagcgcga
    15241 tgcgctgtca ggggaactgg caaaactggc gcaatgctgg cagtcacata acgccccgga
    15301 actggcacag gctactgatg atctttttgc cctgtaccag cgtgcgttta atcgtgcggc
    15361 accggctcct gtccccactc cggcggcctc cggtagttca ccacagacca ccgccacgtc
    15421 tgaaagcggc gtgacgcaac ccagtgctcc ggctccccaa atcgtcatcg acagtcacga
    15481 cgacaaagcc tggcgcgaca cgctgttaaa agtggcggcc attttatgtg aacgccagcc
    15541 ggattcaccg cagggctacc gtctgcgccg ccatgcgctt tggcagaaca tcaccagtac
    15601 gccccaggcg gaaagcgata gccgtactcc actggctgcg gtctccgccg atatggtggc
    15661 agattaccat gctcagctcg gtagtgccga tatggcgctg tggcagcagg tggagaaaag
    15721 cgtactgctg gctccttact ggctggatgg tcactgcctt tctgcacaga cggcgctgcg
    15781 tctgggttac aaacaggtgg cagacgccat ccacgatgag gtcatccgct tccttgagcg
    15841 tctgccccag cttaccgggc tgctgtttaa tgaccacaca ccgtttatca gtgagcagac
    15901 gaaacaatgg ctggcagctt cgcccgacgc caaagttgca ccggttgcgc aaatcggtga
    15961 ggaatcgaag gcagccagag cctgttttgc tgagcagggt ctggaggcgg cgctgcgata
    16021 tctggacatg ctacccgaag gcgatccccg cgatcagttt caccgccagt accttgccgc
    16081 acagttgacg gaagaggcgg ggctggtaca gcttgcgcag cagcagtacc ggatgttgtt
    16141 caggatggga ttgcagatga tggtggcaga ctgggagccg tccctgcttg agcagctaga
    16201 acaaaaattc acggcagaac aataaaagag aacaggagtt tttgtgttca gattaccaac
    16261 accccgacta ctcagcggac tcaaatcagc cctgcgaccg gcgatgcccc ggtttaaagt
    16321 ctctgctttc tggctgctga tactggcgtg gatttttctg ctggtgtgga tctggtggaa
    16381 aggcccgatg tggacgctgt atgaagaaca gtggctcaaa ccactggcga accgctggct
    16441 ggcaacggcg gcgtggggga ttattgccct ggtgtggctt accgtccggg tgatgaagcg
    16501 cctgcaacag cttgaaaaaa tgcaaaagca acagcgcgag gaagccgttg atccgctcag
    16561 cgtggaactg aacgcccagc agcgttatct tgaccgctgg ctgctgcgcc tgcaacgcca
    16621 tctcgacaac cgccgtttcc tgtggcagtt gccgtggtat atggtcatcg gcccggcggg
    16681 cagtggcaaa acgacactgc tgcgcgaggg gtttccgtcc gacattattt atgccccgga
    16741 gggcgcacgc ggcgcagaac aacgcctgta cctcacgccc catgtcggta aacaggcggt
    16801 gatctttgat atcgacggca cactctgcgc tcccgctgat gcggatatcc tgcatcgccg
    16861 tctgtgggaa catgccctcg gctggctgaa agaaaagcgc gcgcgccagc cgctgaacgg
    16921 gattattctg acactcgatt tacccgatct gctgaccgca gacaaacgcc gccgcgagca
    16981 tctgttacag acgctgcgca gccgcttaca ggatatacgc cagcatcttc actgccagtt
    17041 accggtttac gtggtactga cccggcttga tttattacag ggttttgccg ccctgttcca
    17101 gtcactcaac agacaggatc gcgatgcgat tctgggcgtc acgttcaccc gccgtgccca
    17161 tgaaaatgat gactggcgaa cagagttgaa tgctttctgg cagacatggg tggatcgaat
    17221 gaatctggcg ttgccggatc tgatggtcgc tcagactcac acccgcgcgt ctttattcag
    17281 tttttcccgc cagatgcagg gaagccgtga accgctggtg tcactgcttg agggtctgct
    17341 tgatggcgaa aatatgaacg tgatgctgcg tggtgtctat ctcacctctt cgcttcagcg
    17401 tggacagatg gatgatatat tcacccagtc tgccgcccgc cagtaccggc tgggcaataa
    17461 cccactggcg tcctggcccc tggtggacac cgcgccttat ttcacccgca gcctgttccc
    17521 gcaggcatta ctcgcagagc ctaatctggc aacagagagc cgcgcctggc tgatacgttc
    17581 ccgtcgccgc ctgacggttt tctctgccac aggcggcgtg gcagcactgc tgctcatcac
    17641 cggctggcat cactattaca acggtaacta tcagtccggc atcaccgtgc ttaagcaggc
    17701 caaagccttt atggacgtgc cgcctccgca gggggaagat gactttggca acctgcaact
    17761 gccgctcctg aacccggtac gcgatgccac actggcctat ggcgactggg gcgaccgcag
    17821 ccgtctggcc gatatgggac tgtaccaggg acgacgtatc gggccttatg t
//
LOCUS       DAJVQG010000081        17592 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJVQG010000081 DAJVQG010000000
VERSION     DAJVQG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17592
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.081"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..274)
                     /gene="adrA"
                     /locus_tag="OQM83_002448"
                     /gene_synonym="yaiC"
     CDS             complement(<1..274)
                     /gene="adrA"
                     /locus_tag="OQM83_002448"
                     /gene_synonym="yaiC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="catalyzes the conversion of 2 GTP into c-di-GMP;
                     adrA overexpression induces cellulose biosynthesis, cell
                     adherence to abiotic surfaces and swimming and swarming
                     motility; AdrA acts post-transcriptionally on the bcsABZC
                     operon activating cellulose biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCS1520690.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRA"
     gene            complement(376..696)
                     /gene="psiF"
                     /locus_tag="OQM83_002449"
     CDS             complement(376..696)
                     /gene="psiF"
                     /locus_tag="OQM83_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCS1520691.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(815..2230)
                     /gene="phoA"
                     /locus_tag="OQM83_002450"
     CDS             complement(815..2230)
                     /gene="phoA"
                     /locus_tag="OQM83_002450"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCS1520692.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2331..2591)
                     /gene="iraP"
                     /locus_tag="OQM83_002451"
     CDS             complement(2331..2591)
                     /gene="iraP"
                     /locus_tag="OQM83_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCS1520693.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(2773..2976)
                     /locus_tag="OQM83_002452"
     CDS             complement(2773..2976)
                     /locus_tag="OQM83_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1520694.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            3054..4148
                     /gene="ddlA"
                     /locus_tag="OQM83_002453"
     CDS             3054..4148
                     /gene="ddlA"
                     /locus_tag="OQM83_002453"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCS1520695.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALKRHAADNALKATM"
     gene            complement(4172..4384)
                     /locus_tag="OQM83_002454"
     CDS             complement(4172..4384)
                     /locus_tag="OQM83_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCS1520696.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4644..4952
                     /locus_tag="OQM83_002455"
     CDS             4644..4952
                     /locus_tag="OQM83_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCS1520697.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(5011..6105)
                     /gene="yaiW"
                     /locus_tag="OQM83_002456"
     CDS             complement(5011..6105)
                     /gene="yaiW"
                     /locus_tag="OQM83_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCS1520698.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(6118..7338)
                     /gene="sbmA"
                     /locus_tag="OQM83_002457"
     CDS             complement(6118..7338)
                     /gene="sbmA"
                     /locus_tag="OQM83_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCS1520699.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7690..8847
                     /gene="ampH"
                     /locus_tag="OQM83_002458"
     CDS             7690..8847
                     /gene="ampH"
                     /locus_tag="OQM83_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCS1520700.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8848..9471)
                     /gene="iprA"
                     /locus_tag="OQM83_002459"
     CDS             complement(8848..9471)
                     /gene="iprA"
                     /locus_tag="OQM83_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HCS1520701.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(9559..12447)
                     /locus_tag="OQM83_002460"
     CDS             complement(9559..12447)
                     /locus_tag="OQM83_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1520702.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            12973..13947
                     /gene="hemB"
                     /locus_tag="OQM83_002461"
     CDS             12973..13947
                     /gene="hemB"
                     /locus_tag="OQM83_002461"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCS1520703.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(14053..14904)
                     /gene="tauD"
                     /locus_tag="OQM83_002462"
     CDS             complement(14053..14904)
                     /gene="tauD"
                     /locus_tag="OQM83_002462"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCS1520704.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(14901..15728)
                     /gene="tauC"
                     /locus_tag="OQM83_002463"
     CDS             complement(14901..15728)
                     /gene="tauC"
                     /locus_tag="OQM83_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCS1520705.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(15725..16492)
                     /gene="tauB"
                     /locus_tag="OQM83_002464"
     CDS             complement(15725..16492)
                     /gene="tauB"
                     /locus_tag="OQM83_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCS1520706.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(16505..17467)
                     /gene="tauA"
                     /locus_tag="OQM83_002465"
     CDS             complement(16505..17467)
                     /gene="tauA"
                     /locus_tag="OQM83_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1520707.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         4374 a         4561 c         4259 g         4398 t
ORIGIN      
        1 cggccctgct cgctatctgc caggctaaat gcggccagac gaacgcccag ccgaccaaca
       61 ccagccacca ccagcccggc ggcgggtgcg aaaccagcgt tgaagcaatc ggtaagaaca
      121 tgccagccag gccaaccgca cggggtagtc tgacgcgacg ggcgaagcgc agcccggacc
      181 gctgatgttc gttttgagga gttaaaggag gttcctcccc atgcgccgcc gcttttttga
      241 aaaagttttc atcattcatt atttttggga acattcagaa acaattttcc caaattatag
      301 agacggggct aaaggcagaa gtatgaaatt tcgggtgagc cactttcgta gctcacccgt
      361 tagcactatc aggtatcagg cggctttctt gaggcaggca ctcataaact tattacgatc
      421 atcacctttc agagattgtt gcgttgcttg attattacat tcgcgcatct tttgctgctg
      481 tggcgtcaaa cttttttcac caggcgcaga cttgctgttc ttcaggcaat cactcatgta
      541 ggtcttacga gcatcccctt tcaacgcctg cgccgtcgcc tgctgattac aggaggtcat
      601 acgctgttgt tgtggggtta aggttctctc ggcagcgccg acggtggtta aaaaaaccag
      661 accaaaaagc aaggtaacca gtaatgttat tttcatagca ccatccctct tcatgtttta
      721 accatgagcg tttgcgcccg tgatctgcca ttaagtctgg ttgctaacag caaaaaaacc
      781 acccggcagc gaaaattcac tgccgggcgc ggttttattt cagccccaga gcggctttca
      841 tggtgtagaa gagatcggtc tggtcggtca gtccaacaac attggcggca tgcggaccat
      901 acgccgcaat acgcaactga ctgccggtat gttcttgtga atcctcttcg gaattcccgt
      961 aactcatcac catcactgcg ccatctttag tatttagcgc ctgagtgagg cccggcgctt
     1021 tggtatccgg cgcaacaacc tggctggcgt gggcgtgatc agcggtgact atgaccagcg
     1081 tgttaccctc ctttttagcg aattccagcg cccgttgtac ggcttcatcg agatcgaccg
     1141 tctcgccaat ttgcccacaa ggattcgcag cgtgatcctg tttatcgatt gacgcacctt
     1201 caacttgcag gaaaaatcct ttctcatttt tactcaacaa ttcaatggtt ttgtcggtca
     1261 tctgtgccag ggttggtaca ctgtcattac gttgcggatt aggcgtacag gtgactgcgg
     1321 gcttatcgat attgccgtgg tacgttgctt tcggccctag ccagcgcact ggcatattgc
     1381 cgtcagcaaa caggccaagc aggggttttt gctgattcgc ttccgtcacc gaattcagtg
     1441 aggcagcatc gttcaccaac tgataaccac gcgcctgtgc ctgttcacgc agcgtttttc
     1501 cctgccattc accagcggtt gccgtttcag caaaggtttt tgcgccgccg ccaagcgtaa
     1561 cgtcggcacg agcgttaagc agctgttcgg taatcgatcc ttttccgcct ttttccagag
     1621 cgttacccgg acatttttca ctggtcgcgc tcggaccgta gcatttgcgc gaagtcacat
     1681 gtgccaccag cgcagcgggc gtggcatcct gcaactctgc ggtagaaacg ttaccggtcg
     1741 ccagacctgc ggcttttgcc atttccagaa tcgttgggtg atctttttcg tgaatatcga
     1801 cgcccagcgc gccgttatag gttttgacac cggttgacca ggcggttgct gatgcagccg
     1861 agtcggtgac gtagtccggt ttgccagttt ttttattcag cgcatagtga gtgtattgcc
     1921 cggtaagcgg taaggcatcg atacctttaa aaaagccgcc cgcaccttcg gcataattac
     1981 gtgcggcagt aatttccgaa tcccccatcc catcgccaat cagcaaaata atattttttg
     2041 caggtttatc gctaagagaa tcacgcagag cggcggtctg atcactcgtt aagcggcgag
     2101 caccgccggg tgcagtaata tcgccctgag cagcccggtt ttccagaaca ggcatttctg
     2161 gtgtccgggc ttttgtcaca ggggtaaaca gtaacggtaa gagtgccagt gcaatagtgc
     2221 tttgtttcac tttattttct ccatgtacaa atacattaaa aaataaaaac aaagcgacta
     2281 taagtctcgg ccgtgacaac tttatgacag ctgttgaaaa gattaacttt ttactgacga
     2341 ggatgcttca ataacttctt tacgtaatcg cgcagcagct ccgtatcgtc gtcaggaatg
     2401 ctggcatcgg gctttacctc gtacagcgcc ccctctacct gatcaatcaa ccgctgttgg
     2461 tcattttgcg ccatattgcg aagcattgca gtgacgataa tctccaaagc ttctacctgc
     2521 gcacacagtt ctttcgactc ttcttctttt tgggcaagct taaataacaa ctcagcaatg
     2581 agatttttca tgtctgtatt tccttatcca aagtatggag aagttaacat tatgtttcac
     2641 aacttcatag tgtctggaag tattattttt atgacaaagg aaatactttg cgcaaatatt
     2701 ttttgtttga ttaccagcga aacgtttctc ttatttaaat aattacccac acaaaatata
     2761 ggcttttaat tatcaacctt tattataaat aaaagttatg aaatatattc tcttcgttaa
     2821 cttataatta agagaaaaaa actcaccgca ttgccattta agccaggaat aaattagctt
     2881 cccgttcatc atttttgtga tctcgcgcac atctttgaga taacgcactt tcttcaccag
     2941 tacgttgcac gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc
     3001 cgttttgtcg tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa
     3061 aactgcgggt aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat
     3121 cggcaaaaaa cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca
     3181 ttgataaaca agggcaatgg cacgtcagcg atgccagcaa ttatctgcta aatgcagacg
     3241 atcctgccca tattgcgttg cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac
     3301 atgagcatca gcttatcgac gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt
     3361 tcccgattgt ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg
     3421 ccaatttacc gtttgtaggt tctgatgttc tggcttcagc agcctgtatg gataaagatg
     3481 tcaccaaacg tctgctacgc gatgccgggc tgaacattgc gccatttatt accctgacgc
     3541 gcgctaatcg tcacaacatc agttttgccg aagtggagtc taaactgggg ttaccactgt
     3601 ttgtaaaacc ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac
     3661 agtacgcaat tgccgtcgat ctggcgttcg agttcgatca taaagtgatc gttgagcaag
     3721 ggatcaaagg tcgtgagatc gaatgcgcag ttctgggcaa cgacaatccg caagccagca
     3781 cctgtggcga gatcgtactc accagcgatt tctatgccta cgacaccaag tacattgacg
     3841 aagatggcgc gaaagtggta gttccggcag ccattgcacc agagatcaac gataagatcc
     3901 gggcgattgc cgttcaggct tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg
     3961 tgtttttaac cccagagaac gaagtggtga tcaacgagat caacacactg cctggcttca
     4021 ccaacatcag tatgtatccg aagctgtggc aagccagcgg tctgggttac accgatctga
     4081 tcacacgttt gattgagctg gcgctgaagc gtcacgctgc ggataacgca ttgaaagcca
     4141 caatgtaata ttttaacgcc cgataatatt cttattgggc gttttcagtt tcttcatcgc
     4201 gacgacgaat aatcaacccc gccagccaga aactaatcac ccacgtcacc agaccgacgg
     4261 cataggtctg ccaacctttt gcttcaaatc ccagtaaccc caccacgcca ttaagaatga
     4321 atataaggcc aatggcgaac gcatagtagt gccagtcacg gcggattttt acaggcagat
     4381 tcatggcagc tccgttaacg aaaaacaaca tccttgcata ttctgtaaca accgtctgtg
     4441 gtgtagacaa cattttcgta attgttaata aatttaactt aaggttgcca ttttgtaact
     4501 cacaacgaag attcacgctt cgggaatatt cccaacatca atttaccatg attctgatat
     4561 tgacaatcta aatgacctgt cagacttgcc ataagccgct ggtatcacac cagaagagaa
     4621 gcagatgact tccggaggtt tttatggctg atttcaccct gtcaaaatcg ctgtttagcg
     4681 gaaaatatcg caatgcctct tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt
     4741 tttgcttttg ggctggggcg caattgctga acctgttagt gcatgcgccc ggcgtctatg
     4801 agcgtttaat gcaggtccag gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg
     4861 gcaccatttt cgggctgatc ccgtttttag taggctgcct catttttgca gtggtggcgc
     4921 tatggctgca ctggcgacat cgccgccagt aagatttacg ttgccagata cggcctaaaa
     4981 cgccttatct ggcatgacat tcctgtttga tcactgtttc atacaacgcg cccgccgttc
     5041 gtctacgcgc ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg ggctttccag
     5101 ttgaatgcca ggcaacattt cgcggggtag tgatttaccc gttttcgctt cggcaagttg
     5161 ataaactttc ttgtacagcg ccgtctcctc aaaagagaag ctgtcacctt tctctaactg
     5221 acggcgaatt tcgctgtcgc tcatccccag ttttcccgcc agtttgcgcg ttgccagttc
     5281 ggttttcccg ggttctttac tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac
     5341 gccgctggcc ttactgactg cgttctgaaa cgcggcatta cggctggcgt accagccagc
     5401 gttaaaatca gcaaagcggt atatcggtgc gctatagctg gcgggataat tcagtaaatg
     5461 gtaagtacca aaccacaacc cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc
     5521 cattttccac ggataccctt tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg
     5581 cccaccggtg cgcaccggat taagcgaacc aaacagcgtc tgccccattg gtaccatgct
     5641 gatcagatcg tcaaaaatcg cgctcaattg cttttctgta cgcaccgaat ctaaccgttc
     5701 gctataactt ttgccgtttg gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg
     5761 aatgtgcatc cgttcagcac gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg
     5821 aacggccgga tctgcctgat aatttgactc ctgctgcgcc accgccagca ccgagcagac
     5881 gttttccagc gtcggtgcca gcccctgact ttcaaaggtg atggcgagat ctttcgccca
     5941 ggcatcgcgg tctttcacgc tcgcaggcat cttctgccgc acgacgctcg ctacatctat
     6001 cgctttttcg ccctttttca acggttgcgg agcctgacta ctacaccccg ctaaaaccag
     6061 aaccgccaac aatgacagcg aagatagatg attgacgcgc gacattactt ctccttttta
     6121 gctcaaggta tgggttactt cctgaatttt gtcaccatcc agctcatgtt caaagctgcg
     6181 cagacgtttg tagatagaca tcaactcaac cagtgtggtc catgagttaa tcaggtactg
     6241 gaaagcaccg cgaacctgac caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat
     6301 cgtaccggca acaattgacg gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa
     6361 atagagaatg cgggcgatgt tgaaatacat atagtgaaaa tagaggcgaa aatagttttt
     6421 ccgtacggcg ctaaacagct cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc
     6481 accataaacc agctctttac ggtaggcggc ctctactcgc tggtttttaa actccagccc
     6541 cggcagtttg atccctacca ctgccagcca tccggtcccc atcagcgacc agacgatagc
     6601 ggcaatcacc agaccatacg gaatgtgccc gataatcggc agctccggca catgcgcgga
     6661 gagcgttacc agcaccggca ggaaggcgat caacgtcatg atggcgttga taaaactgac
     6721 gcccatattc tccagcgttg aagcaaaacg catggtgtct tcctgcacac gctgtgcggc
     6781 accttcgata tgacgcagtt gttgccagtt cgccatgtaa tattcgttca tcgccgtacg
     6841 ccagcggaac acatagtgac tgacaaagaa gttgttcagt acactgatca ccaccgcgat
     6901 cagcgcaatc cccagaaaga cgcccacttc gcggtaaaat tgttcgatag tgactttatg
     6961 cggcgaactt agcgcggttt gaatcagatc atagaacggt gcataccagg cgttgacggc
     7021 gaccccgact tccaccaaaa accaggtgac gaagatgatc agtgcagtac cgagtattga
     7081 ccagtattgc caacggtgcg ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca
     7141 aacaatgtag taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg cggcgctaat
     7201 cgggatctgc ccggaagcgc cggtgatacg cgccacccag tctcccccac cggcttgcca
     7261 gaagataacg gcaatcaatg cccaaacaaa ggccgagaga aaaaacgctc ccggctttgg
     7321 gaaaaaagac ttaaacatat gcactcctgc taacttctta tcgttttaac aactgctgtg
     7381 tattaaccgc atgaccacta caaagcgtca gccaaatgaa accgcccatt gtgacccata
     7441 gcaaggcgct tagttcgcca gcctgaccat aagaattgtt tcgacggatt tcacaaacca
     7501 aagattgttt acataagcgg attaacacgc gcaggatcac aaatgcaggc attggtgagc
     7561 aggaaaaaga tcgtgtttac gcgccgttac gtttttaaca ctattattgc actaattcgc
     7621 cctttgcaat ccatcaatga gtagtataaa tacgctcagt taccttcatt caatctatgg
     7681 acaccaccgt tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc
     7741 tccgtccatg cggcgcagcc gatcactgaa ccggagtttg cctctgatat tgtcgatcgt
     7801 tatgccgatc atatttttta cggcagcggt gcaacgggga tggcgctggt tgttatcgac
     7861 ggtaaccagc gcgtctttcg tagttatggc gaaacgcgac ctggtaataa cgttcgcccg
     7921 cagctggatt cggtcgtgcg tattgcttcc ctcaccaaac taatgaccag tgaaatgctg
     7981 gtgaaattgc tcgaccaggg gaccgtaaag ttaaacgatc cgctaagtaa atatgctccg
     8041 ccaggcgcac gcgtgccgac ctacaatggg acgcccatta ccctagtgaa tctggcaacc
     8101 cataccagcg ccctgccccg tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc
     8161 tggccaacgc gcgagcaacg ctggaaatac ctttctacgg cgaagctgaa agccgcgcca
     8221 ggcagtcagg cggcgtactc taaccttgcg ttcgacctgc tggcagatgc actggcgaat
     8281 gcgtccggca agccttatac ccagttgttt gaagagcaga ttacccgtcc attggggatg
     8341 aaagacacca cctacacccc ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc
     8401 ggtgccagtc cgtgcaataa cacgctggcg gcaattggca gcggcggcgt ctattccacg
     8461 cctggcgata tgatgcgctg gatgcagcag tatttgtcat ctgattttta tcagcgcagt
     8521 aaccaggcag atcgcatgca gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt
     8581 ggcatggatg tccccggcaa agcggatgcg cttggtttag gctgggtata tatggccccg
     8641 aaagagggtc gtccggggat tattcagaag acaggcggtg gtggcggttt cattacctat
     8701 atggcgatga tcccgcagaa gaatattggc gcgtttgtgg tggtcactcg ttcgccgttg
     8761 acgcgcttta aaaatatgag cgatggcatc aatgatctgg tgaccgagct aagcgggaat
     8821 aaaccgttgg ttatccccgc atcctgatta atactctgaa ggcaaacggt tgatagcgac
     8881 cagtttgcct ttattcattt cgatatagcc gcctttacgc aaagcagcga gaacttctgc
     8941 gacgacagaa cgcgatatgc gtgtacgttg atggatataa ttcatcacac caatacgtga
     9001 tcgcaattct tcattccagt caatcattga taataatgtt gcgcggattt gttcgtagga
     9061 attatgccca atgagctgca cgtcgcgtaa ttccagaatt ctattttgcc aagctaacca
     9121 gtaaaaagcg tctcgccaga gttgattttg ttcaataagc gtaatggttt gtttggctgg
     9181 tagatgatat cccgtacaat ttccttctga tattaatttg tatggtatat cgtttttcat
     9241 taaaccatca gccagcccca taatataagg ggcctgggta ataccgataa gtacgttttc
     9301 ttctctacgc agagagataa ctccctctaa aataacgaaa gtatcttcgt tattgacatc
     9361 actggaaaat ataacttgct tttcattatt aaactcgaag cgcgtaccgt atctggacaa
     9421 acatttatcg agcttaccaa attcctgaag aggtttaact acagataaca tttgcgcgtc
     9481 ctttgcagta atgcccgtca aatccttgac gggcattatt tagattaaat taccagtatt
     9541 tcttcggagt gaagaatatt accaggtata tttaacaccc acgttcgcgg accagtcttg
     9601 atctacgtca ccaccaccga ggtagttagc atcggtatag gcgctgaagt tcttggtgaa
     9661 gctgaactga gtacccagac caacacgtac cgcagaccct tcagtaccgt tatcgatgga
     9721 atcaccgttc acatcgttat cgttgttaga gtcgtcgtag acgtaagcca gtttgaagta
     9781 cggagtcaga gcctgatctt cgctgtaggt gaaggtataa cctgcatcta cacccagttc
     9841 ataacgcatg ctgtcgtaag actgaccgtc aactttcatg tcgttgctca gctggtagtc
     9901 atcaccagac tggaacagac cagaaacgct gccgtaagga gtcacgtaac cagcatcacc
     9961 cagtttgaag tcgtaaccgg ctttcaagcc gaagccccaa gcgtcggagt tggtgctacc
    10021 gtcaacgtaa gtaccgttgc tcatggttgc agacaggtcg ttgttgaagt gagagtagct
    10081 caagctacca tcaacaaaga cgttgttcgc gaagtgagca gaagagtaga tgtaggcagt
    10141 ctggctgtct tgatctacct gaccagaacg gtcattcatg tcacctttag cgaagcctgc
    10201 agccgcaccg acgatccact tagcgttgtt accgtcaact ttggtatcaa caccgaccat
    10261 gatgccgtta acatcctgat catagttgat ggtgccgttg tcgccgttga agttaccacc
    10321 gaagtagctt acccatgcgc cgccgttatc agccaggcca tgacgagagt tggtcagacg
    10381 agtaccaacg gtgtcttgtt ccaggttcca gatattggtg ttcgcagacg ggatgctcag
    10441 cgccatgtta gcgtagtcgg tcagctccat ctgttgcaga acaacggtgt taccgcgctg
    10501 ttcagcctga taggtgtatg cacccaggtc agctttgtta gcagcagaga aggtcgcgtt
    10561 ggttttggta tcatcgatat agatgagctg cttacctgcg taatcagcaa tcgcaccagc
    10621 gccagtggag ttgtcgatac gaactttgta gttaccggtt gcagctacaa cgttgtctgc
    10681 agagctgttg tcaacttcgt cgccattgtt aatgtcgttg ttaccattaa cggtcaagtg
    10741 accatcagag ttcatcgcaa taacaccata accgtagtta gctttgctgg tgtcgttggt
    10801 acgatcgttg acgaggtctg cattcaggat gtaatcatta ctatgaatgt caaacacacc
    10861 tgcgtaaaca ttaccgttgt tatctttaac gttgccactg gtcagttcca gagtatcagt
    10921 tgcaaatacg ctggagatgt cattcatgtt ggtgtttaca tccagcacac caccgttagt
    10981 aacggtaatg gtattggcat acagcggtgc atatgcccaa gctgaagcag tatcttcacc
    11041 gaagttaacg gttgagaagg tatcaatggt caggtgatcg gtgttaactt caccgttaac
    11101 ttccattgaa gaaccaccgt tcagagtgat ggtgtcagaa gtcagagaag aatctgcact
    11161 cacgttaacc tgagaaccgc tgttgatcgc cagggtatcg atcagggaag ttttcgtggt
    11221 ttcccaatca gaaccgttgt tcagaacaac atcgaatacg ccgctctgga atgcgttagc
    11281 gttatcaaca cgattccagc catcttcaga gtaagaagcg tacgggttca ggctgtttgg
    11341 tgcaacgtcg tagaaggctt cgtcagcaac aacaacgtca taatcaatat tcgctgaacc
    11401 aacccatttg gtgttgttca gagtcaggtt caggtgatcg acgttctgat cgtcagttgc
    11461 ccaaccgcca tgagtgtaag cagtggtaga gttgtcaatc aggtgacccg tcgcgtcaaa
    11521 gttagcgttc caggtactgt caaatacaac gtcaccaagc aactgagagt tgttgaacgt
    11581 aacgttgttg accatacgat aatcagaagt gtcatcatcg atgaaggcta acgcaacatc
    11641 atcagcagga gatgcgccat tgccgtagtc acttggttca acagaattgc cgaaatgacc
    11701 acgatctgat tgttcgtctt cagtccatga accagaagtt acagtagagt cattaacaac
    11761 gatgttgttg tcccactgat gaccttcggt attgtaggtc gcattaccga ggtcgtgttg
    11821 aaccaaagta atacctgcaa catgggagtt gttgctgatg ttgatgttac tttcaacatc
    11881 cagagtcaca gcggtaccaa gaccagcggt taccaaacga tcataatctg tcttagatgt
    11941 tttaccgtct gcatccagat aagtatcggt gaagtagaag gcgtcaaaat catcgtcaat
    12001 agtagaatta gcgatgttca gagtgaagat gtcgccatca acccagtcgt ggccagtata
    12061 atcgctaccg ttggtccaga cgtagtcgcc ataaccaacc tggtaagatg taaccatacc
    12121 atgaacaatt gagttggaga tgttcagtgt attaccggta tctgtgccat aatcgtggtt
    12181 cagataataa gtagaaatta caccatttac agtgtcgccg ctgaaagtgg tatcaaagta
    12241 accatcagct gcgtggtcat atacataacc accgtaagtc agataatcgt gatcaatttt
    12301 agtgctgtca ccccaattgt agatggtggc tgcgtcatcg tgatacccga aggtgttata
    12361 agtagctgca ttagcctgag aggtgatagc cagagtgcaa gccaatgcta attgtgatac
    12421 tacaagtttc tttttccagg agtgcatttg tcatccctcc tcagggacgt aataagttga
    12481 tccatcaatt atcaatgcat agaaaacata ttatgtttcg cgctaacgat tatgctgcgt
    12541 tacagacgaa tggttcaatt attctttccc ctaagtccga atgtggacat atataattta
    12601 tggagaaaaa cacgcatcaa attgataaaa ggacgatgtt tcaacatgtt gcagataatg
    12661 ttatttttga tacatacgcg ttataaatat caaaaacaca aaccagataa atatcttatc
    12721 aatatacaac aaagtttgaa ttattcaatt tatagcagca caaccaaacg ccaaaaaaaa
    12781 taataaccaa gagctataaa taaagattaa aaatattttc agtgaatatg aaattattgc
    12841 tatttctatt acaaagatgt cctggtctct ctttcattct cttcttgtca acatcacgac
    12901 aactttcgta aaacactcct gccccgcttc aggtatactg tgcaccttaa ttccacaaac
    12961 atcaggcaga ccatgacaga cttaatccaa cgccctcgtc gcctacgcaa atctcctgcg
    13021 ctgcgcgcta tgtttgaaga gacaacactt agccttaacg acctggtgtt gccgatcttt
    13081 gttgaagaag aaattgacga ctacaaagcc gttgaagcca tgccaggcgt gatgcgcatt
    13141 ccagagaaac atctggcacg cgaaattgaa cgcatcgcca acgccggtat ccgttccgta
    13201 atgacttttg gcatctctca ccataccgat gaaaccggca gcgacgcctg gcgggaagat
    13261 ggactggtgg cgcgtatgtc gcgcatctgc aagcagactg tgccagaaat gatcgtcatg
    13321 tcagacactt gtttctgtga atacacttct cacggtcact gcggtgtgct gtgcgagcat
    13381 ggcgtcgaca acgacgcgac tctggaaaat ttaggcaagc aagccgtggt tgcagctgct
    13441 gcaggcgcag acttcatcgc cccttccgcc gcgatggacg gccaggtaca ggcgattcgt
    13501 caggcgctgg acgctgcagg ctttaaagat acggcgatta tgtcgtattc gaccaagttc
    13561 gcctcttcct tttatggccc gttccgtgaa gctgccggaa gcgcattaaa aggcgaccgc
    13621 aaaagctatc agatgaaccc aatgaaccgt cgcgaggcga ttcgtgaatc actgctggat
    13681 gaagcccagg gcgcagactg cctgatggtt aaacctgctg gagcgtacct cgacatcgtg
    13741 cgtgagctgc gtgaacgtac tgaattgccg attggcgcgt atcaggtgag cggtgagtac
    13801 gcgatgatta agttcgccgc gctggcgggt gctatagatg aagagaaagt cgtgctcgaa
    13861 agcttaggtt caattaagcg tgcgggtgcg gatctgattt tcagctactt tgcgctggat
    13921 ttggctgaga agaagattct gcgttaattt ttttcctctc agaaaggggg agttaacctg
    13981 ttgaaagtac ggtgaacgcc ccctcaccct aaccctctcc ccaagggggc gagggaaccg
    14041 tccgcactcg tatcaccccg cccgataaaa cggtttatcc ccaaggatcg tcgcccgatg
    14101 cataatccgc cgctgtggca ggtaatcggc attggcatag tgctgagtca cgcggttatc
    14161 ccaaatcgca atatcattcg gttgccagcg ccaacgcacc tgaaactccg gtttagtgat
    14221 atgggcgaat aaaaagccta acaaggcttc gctctctttc tcgctgacat cgacaattcg
    14281 cgtagtaaag ccttcattca caaacagcgc ctgtttaccg ctcaccggat gcgttcgcac
    14341 caccggatgt agcaacggag gatttttcgc gaccgcctcg cgccagcgtt gatgttcctc
    14401 ctcggttttg cggtatttgt attccgggaa cgatttacgg aaatcatgct ccgcacgcag
    14461 cccgctcagc agctggcgga agggaacaga gagcgcctca taggccgcaa taccgctggt
    14521 ccagagcgta tcaccgccgg tcgaaggtaa ctctttagct gccagaatcg cccctgcggg
    14581 tggcgtttca ataaatgtca catcggtatg ccagttgtcg ttatccggcg gattatcgtt
    14641 atgggtatcc agcacgatga tctcgtcaac cccttcggca tgagggtaaa cagggtgaat
    14701 atgcagttcg ccaaaacgct gggccagcgc gcgttgctgc tgcggcgtaa tagcttgatc
    14761 gcgcagaaat accacctgat agcgcagcac cgcatggtaa agctgttcaa actgattatc
    14821 gcttaacggg cgcgtcaggt cggcacccga aatttgtgcg ccgatatacg gccccagcgg
    14881 ggtaatactc agacgttcac tcattgtact tctccatgcc agggcgtcag acggcgctgt
    14941 aacgcgcgca gacccagttc taaaagaaag gcgataatcg caatcaccgc gatccccgcc
    15001 agtaccacgt cagtcgcgag aaattcaccc gctgactgaa ccataaatcc taaaccgcgt
    15061 gtcgcggcaa tcagctccgc cgctaccagc gtagaccagc ccacacccag accaatgcgt
    15121 aatccggtga ggatttccgg cagcgcgccg ggcaaaatga caaaccacag cacctgcgca
    15181 cggctggcac ccagcgactg ggcggcacga atgcgaacct gctgcgcgct tttcaccccc
    15241 gccagcaccg acatcgccac cggtgcaaaa atcgctaaat agatcaataa gatcttcgag
    15301 gtttcaccaa taccaaacca gatcaccatc agcggcaaat aagccagcgg cggcaccgga
    15361 cgataaagtt cgattaccgg atccagaatg ccgcgcaccg tcgggcttag ccccatggcg
    15421 atcccgaccg gaataccgat caccaccgcc gcgagtagcg ccagcacaat gcgcgtcagg
    15481 ctggctgcca gatgctgcca cagcgtggcg tccataaatc cctgcggtcc ggcaatggtg
    15541 agcagttttg ccagtacttg ttgcggcggc ggcaaaaaca gcgggctaat cagttgcagc
    15601 gccgccaccg tccaccatac agtgagtaaa accgctaacg tgccaatgct taaggtcacc
    15661 tgacgcgaga gcggccagcg ccatttcagc cgccgcgaat gcagtttttc attaatgagc
    15721 acactcatga gaacgcctcc cgttgctcaa atacgcggct taaaacatat tcgcgcatgg
    15781 cgatgaattg tggatcggac ttgatgctgc ggctcgactc tcccgcaaca aagcggcgag
    15841 caaagttgag cggcagccgc tccagcacac ggccagggcc ggatgaaagc agaaccagtt
    15901 cagtcgccat aaacaccgct tcttctatat cgtgggtaat caacagcacc tgcttgcccg
    15961 tctcctgcca gagtttcagc agcagagttt gcatctggtc gcgggtgaag gcgtccagcg
    16021 caccaaacgg ttcgtcgagt aataacagct ggggattcgc cgccagcgcg cgggcaatcc
    16081 ccacccgctg acgttgacca ccggaaagct gccagatata gcgtttttct gcgccttcca
    16141 gccccacttt tttcagcatc tggtgcgcga tttccagtcg ctgcattttc tctatacctg
    16201 ccaattgcag gccgaacgcc acgttgtcct gtacattgcg ccacggtagt agcccttcat
    16261 tctgaaaaac caccccgcgc tctgctcccg gtccctcaat acgcttaccc gccagttgaa
    16321 tgctgccatg ctgataaggc acaaaaccgg caatcagatt cagcagggtg gttttaccgc
    16381 agccggacgg ccccagcacc accagtagct cgccgctttc cagcgtcagg ttgatatctt
    16441 ccagtgccgg tttgccgcca taatcggcat agagatgaga gatttgcagc atctgcgcct
    16501 ccttttattg cacgaagcgc gaggtaacgt actggctgta atcattcgct acagccggga
    16561 ccttgccctg ctctttcaaa aactgcgcgg tgtcgatgat tgctttgttc accggtccgg
    16621 tcagttctgc cgtttgttgc tgcggcgtca gataggtatt ccccttcacc agccccggaa
    16681 cgtcaccttc cggcacgccg cttaaacgcg ccagtttgct gatgttttcc ggctgtttca
    16741 gccacgcgtc tgggttagca atgtacggtt gctgagcatc gatggcgctt ttagcgaacg
    16801 ctttcacgac ctcaggatgt ttctcggcaa aatctttgcg caccacccag acgtccagcg
    16861 ttggcgcacc ccactgcccg acctgttcag aatcggtcaa caccttgccg tctttttcca
    16921 gggcgttaac cgccggtgcc cagacataag caccatcaat atcaccccgc tgccatgcgg
    16981 cgataatcgc gggcggctgc aggttcacaa tctccacttg ccctggttta atcccccagt
    17041 gtttcagcgc cgccagcagg ctgtagtggg tggtggagat aaacggtacg gcaatgcgtt
    17101 tgccaatcaa atcttcgggt ttgctgatag ttttctttac caccagcgct tcggagttac
    17161 ccagttttga cgccagcaag aagacttcaa tcggcacctg ttggctggct gcaaccgcta
    17221 atgggctgga accgaggttg ccgatttgca cgtcgccaga agccagcgcc cgcacgatgc
    17281 tggctccgct gtcaaactta cgccagtcca cgctcgcccc gctctcttta gcaaaggtgt
    17341 tgtcggcctg agccactttc gccggttcgg ctgaggtttg atacgccacg gtgacgttca
    17401 ccgcctgtgc ctgaaaagcg atgaatgcca gtgcggcaag aagtgtgtta cgcgatgaaa
    17461 ttgccatgat tgtctgctcc cctgtcttgt tatgggagca gtattcagga ataaaaacat
    17521 tcattaaaag aattagtcgt tatcgcacag atgattttat tcttagcaaa aaaacggcaa
    17581 aggaaagatt aa
//
LOCUS       DAJVQG010000082        17515 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJVQG010000082 DAJVQG010000000
VERSION     DAJVQG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17515
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.082"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(57..1031)
                     /gene="cysB"
                     /locus_tag="OQM83_002466"
     CDS             complement(57..1031)
                     /gene="cysB"
                     /locus_tag="OQM83_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCS1520708.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFTIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(1241..3838)
                     /gene="topA"
                     /locus_tag="OQM83_002467"
     CDS             complement(1241..3838)
                     /gene="topA"
                     /locus_tag="OQM83_002467"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCS1520709.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4218..4469
                     /locus_tag="OQM83_002468"
     CDS             4218..4469
                     /locus_tag="OQM83_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCS1520710.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4505..5554)
                     /gene="sohB"
                     /locus_tag="OQM83_002469"
     CDS             complement(4505..5554)
                     /gene="sohB"
                     /locus_tag="OQM83_002469"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCS1520711.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5774..6532
                     /locus_tag="OQM83_002470"
     CDS             5774..6532
                     /locus_tag="OQM83_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCS1520712.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6529..7119
                     /gene="cobO"
                     /locus_tag="OQM83_002471"
     CDS             6529..7119
                     /gene="cobO"
                     /locus_tag="OQM83_002471"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCS1520713.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(7159..8031)
                     /gene="rluB"
                     /locus_tag="OQM83_002472"
     CDS             complement(7159..8031)
                     /gene="rluB"
                     /locus_tag="OQM83_002472"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCS1520714.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QASGGRRSGGRNNG"
     gene            complement(8244..9358)
                     /locus_tag="OQM83_002473"
                     /pseudo
     CDS             complement(8244..9358)
                     /locus_tag="OQM83_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9386..10006)
                     /locus_tag="OQM83_002474"
     CDS             complement(9386..10006)
                     /locus_tag="OQM83_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCS1520715.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(10003..10884)
                     /gene="yciV"
                     /locus_tag="OQM83_002475"
     CDS             complement(10003..10884)
                     /gene="yciV"
                     /locus_tag="OQM83_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCS1520716.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11022..11066
                     /gene="trpL"
                     /locus_tag="OQM83_002476"
     CDS             11022..11066
                     /gene="trpL"
                     /locus_tag="OQM83_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCS1520717.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    11043..11136
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            11158..12720
                     /gene="trpE"
                     /locus_tag="OQM83_002477"
     CDS             11158..12720
                     /gene="trpE"
                     /locus_tag="OQM83_002477"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCS1520718.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12720..14315
                     /gene="trpD"
                     /locus_tag="OQM83_002478"
     CDS             12720..14315
                     /gene="trpD"
                     /locus_tag="OQM83_002478"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCS1520719.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14316..15677
                     /gene="trpCF"
                     /locus_tag="OQM83_002479"
     CDS             14316..15677
                     /gene="trpCF"
                     /locus_tag="OQM83_002479"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCS1520720.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15689..16882
                     /gene="trpB"
                     /locus_tag="OQM83_002480"
     CDS             15689..16882
                     /gene="trpB"
                     /locus_tag="OQM83_002480"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCS1520721.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            16882..>17515
                     /gene="trpA"
                     /locus_tag="OQM83_002481"
     CDS             16882..>17515
                     /gene="trpA"
                     /locus_tag="OQM83_002481"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCS1520722.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQG"
BASE COUNT         4178 a         4663 c         4395 g         4279 t
ORIGIN      
        1 gagaatatat tccggcacct tcgctacata aaaggtgccg aaaataacgc aagaaattat
       61 ttttccggca gttttatatc tttaaacatg acctcaattt cttcattaga gcgcaatgcg
      121 acagccgcat caacgacatc acgcgttaaa tgcggtgcaa aacgctgaat gaaatcatac
      181 atataactgc gcaagaaagt actacggcga aaaccaattt tggttgtact gtggctgaag
      241 atatcgtgag catcaacacg cacaaggtcg ggatcggcga ccggatccac cgccatgctg
      301 gcaatgaccc ctacccccag ccctaaccgg acgtaagttt taatgacgtc agcatccgtt
      361 gccgtgaaga cgatacgcgg cgttaaccct gcgcggttaa aggcagtatc cagttctgaa
      421 cgtccggtaa agccgaaggt atatgtcacc aacggatatt gcgccagttc ttcaatggta
      481 atggcttttt tgcctgccag cgggtgatcc ggagtgacta caatagcccg attccagtgg
      541 tagcacggta acatcactaa atcttcatac agatgcagcg cttctgtggc gatagtgaaa
      601 tcagcattgc ctttagagac ggcatcagca atttgtgtcg gcgagccctg gtgcatatgc
      661 aaagaaacgc ggggataacg ctcaataaag cctttgataa cgtttggtaa cgcgtagcgt
      721 gcctgggtat gcgtggtggc gatatacagt gagcctttat ccggccaggt gtgctctccg
      781 gcaaccgatt ttatggcatc gactttcgac aggacttcgc gagcgatacg aattatttct
      841 tgccctgctg gcgttacctg cgtcaggtgc ttgccgcttc gggaaaaaat ttgaatgcct
      901 agctcgtctt ccagcattct gacttgttta ctgatcccgg gttgtgatgt gtaaagtcct
      961 tccgctgttg atgagacatt caggttatga ttgaccacct caacaatata gcgaagttgt
     1021 tgtaatttca tgttaaacca tccacttaga cgccatcaga acatcgtttt ttcgtcacac
     1081 ctggcgggtg actaatacga tttaataata aaaaaggtgc taactataac cactatatca
     1141 tttatagcct tagtgtatag gtgtgaagaa aaaataatat gcaataaaaa agggccgctt
     1201 tcgcgaccct ttgtttataa aaacctgaca gaattaaagg ttattttttt ccttcaaccc
     1261 atttgccatc aacataaaat gctgaccagc cagtcgcctt tccgtctttt tccgaagaga
     1321 catattgctg tttggtttta cggctaaagc gaaccatggt cttattacct tccggatcct
     1381 gctgtggcgc atcggccaga taacgcagtt tttccggcag acggtcgcgg aagcgataaa
     1441 gctcttccac cagtggcgca cgcgtttcac gcgatttcgg gaaagtgttg gcagccagga
     1501 aaacacccgc agcgccgtca cgcagcacga aataagcatc tgatttttcg cacggcagct
     1561 caggtaatgg caccggatct tctttcggtg gtgccacttc gccgttacgt aaaatcttac
     1621 gtgtgttttt acactcttcg ttggtgcagg ccatgtattt accgaaacgc cccattttca
     1681 ggtgcatttc agagccacat ttttcacact caacgatcgg gccgtcataa cctttaatgc
     1741 ggaattcgcc ctcttcgatc tcgtaaccgt cgcaggttgg gttattacca cagacatgca
     1801 acttacgttt cggatcgatg agatagctgt ccatcgccgt gccgcatttc gggcaacgac
     1861 gttttgcgcg cagcgcgttg gtttcagcgt cttcgccttc cagtacgttc agcacttcgt
     1921 tttccggcac caggttaatg gtggttttgc aacgctcttt cggcggcagc gcataaccag
     1981 aacagccaag gaataccccg gtgctcgctg tgcgaatccc cattttgcga ccacaagtcg
     2041 ggcagtcaat gctggtcaga accatctggt tcgggcgcat accaccctct tccggatctt
     2101 tttcagcttt atctaactgc tgggtgaaat ccgagaagaa gtgatccagt acagctttcc
     2161 actctgcttc gtgatttgcc accaggtcga ggctgttttc catctgtgcg gtgaagtcgt
     2221 agttcattaa ctcgcggaaa ttttcttcaa ggcgatcggt gacgatttcg ccaatttttt
     2281 ccgcatagaa acgacgattt tctactcgca cgtagccacg atcctgaatg gtcgaaatga
     2341 tcgacgcata ggtagacgga cgaccgatac cgcgtttttc cagctcttta accagcgatg
     2401 cttcactgaa acgggctggc ggcttggtaa agtgctgggc tggtgtaagt tcaacgagcg
     2461 tcagagcatc gcctttatta actgctggta agatgcgatc ttcatcgcct ttacgcaacg
     2521 caggcatcac ttttgtccag ccatcaaaac gcaaaatacg accgcgtgct ttcaggcgga
     2581 aatcgcccgc accaatggtc agcgtcgtgg agtcatattt cgctggggtc atctggcagg
     2641 ctacgaactg acgccagatt aactggtaca gtttctgcgc atctgcttcc atatccttca
     2701 gcgattccgc catcacattg acgtcagaag gacgaatcgc ttcgtgcgct tcctgtgagt
     2761 tttctttgct ggcgtactga ttcggacttt ccggcagata tttcttacca aaattatcgc
     2821 tgatataacc acgaaccata tttaccgcgt cctgactcag gttagtggag tcggtacgca
     2881 tgtaagtgat atagcctgct tcatacaaac gctgcgccat catcatggtt tttttcacgc
     2941 caaatccaag acgggtgctg gcagcttgtt gcagcgtaga ggtaataaaa ggagcgccag
     3001 gtttactggt tgtcggtttg tcttcacgtt ccagcacgct gtagcgcgct ttttccagca
     3061 gacttaccgc agcctgagtt tgttctttgt tgaccggacg gaacggtttg tcgttctgat
     3121 gagtcacctg taacgccaac gcttcaccag atggcgtggt cgtgctggca tcgacttccc
     3181 agaactcttc cggcacgaac gctttaattt cacgctcacg ctcgaccacc aggcgaaccg
     3241 ccaccgactg cacacgaccg gcagacagac cacgagcgat ctttttccat agcagcggcg
     3301 aaaccatata ccccaccacg cggtccataa agcgacgcgc ctgctgggca ttaacacgat
     3361 caatattcag ctcacccggt ttgttaaatg cctggcggat cgcgttttta gtaatttcgt
     3421 taaacaccac tcggctatag cgcgcatcat cacccccaat cacttcccgc aggtgccatg
     3481 caatggcttc cccttcgcgg tcaaggtcgg ttgcgagata gatgtggtcg gctttttcag
     3541 ccagttgttt cagttcagag acgaccttct ctttaccagg caacacttca tagtgcgcct
     3601 cccaattgtg ccacgggtca acccccatac ggttgacgag agcgccacgt tcatcctttt
     3661 taggcttttt agccgtcttg gtggaggtag agtcggcact ctttttggca gctgagccac
     3721 tggtcggcaa atcgcggatg tgaccgacgc tggatttcac cacgtagtca ctacccagat
     3781 acttgttgat cgttttggct tttgccgggg actcaacgat gacaagagct ttacccatat
     3841 tcaccttacc taatttgatt cttccaggaa tgcgtcgcac gttgataaac cttccactgg
     3901 caacgagtta ccgatatgga ctcgttctct gcggatatca accccttgtc gtgactctgt
     3961 tcacgaaaat tcaggtgact gagttaacag gtcttggcct acaggcgcta tagcacgacg
     4021 gaattttggt cgaatgtcaa gcaaatctat tgccagattg acgaaagcgt cacactgtac
     4081 ctgataaaat ccgatacgca actttattag catgcaaaat ccaaagttgc cagtaaggcg
     4141 ctcttgccac cagcgtggta tatttgtctg caagccatgc ctggcgtaga ctaatgtcct
     4201 tgtttaagga gtaaataatg aacaaagaga cacaacccat cgaccgcgaa acgctgttga
     4261 aagaggccaa caaaatcatt cgtgagcatg aggatacgct cgcagggatt gaagccaccg
     4321 gcgtcacgca gcgtaatggc gtgctggtat ttatcggcga ctacttttta gatgaacaag
     4381 gattgccaac agccaaaagc accgcggtgt ttaatatgtt caaacacctg gcccatgtgc
     4441 tttcagaaaa atatcatctg gttgattaaa gcaaaacgcg aggtcttagc ctcgcgtttg
     4501 tcttttacat caatggcttt tgaccccgct gccaccagcg tagcaacaat cgatcggcgc
     4561 tctctgccgc gctgccggtg aatcggtcaa tgagtcgttt acgctgcata tagcgtacat
     4621 taaccacttc acggccttcc atcaggctaa gaataacttc atcactggtg ttgatttcat
     4681 caaccaggcc tttctctacc gcctgttgtc cgtaccagtg ttcacccgtt gccacctgtt
     4741 caatatccag agacggacgc atacgcttca caaaatcttt aaataactga tgcgtttcgt
     4801 tcagctcttc gcggaatttc tcccgccctt cttcggtatt ttcacccagc aaggtcagcg
     4861 tgcgcttata ctgcccggcg gtgtgcagtt cgatatcaat atctttgctt ttcaggaagc
     4921 ggttaaagtt gggcatttgc gccaccaccc caatggaacc cacaatagca aacggtgcgg
     4981 aaacaatttt gtccgccaca caggccatca tgtaaccgcc gctggcagcg actttgtcta
     5041 ccgtaacagt taaaggaatg tttttatcac gcagacgctg cagctgcgaa gccgccaacc
     5101 cgtaaccatg caccatgcca ccagggcttt ccagacggag cacaacctga tcctgcggtt
     5161 tgaatgctgc gagtaccgcc gttatctctt cacgtagcga gttcacttca tgggcgtcca
     5221 tgctgccttt aaaatccagc acccagacgc gaggtttact gtcagttgcc acctcgccca
     5281 gtttggcttt cgcttttgct gctttcgctt cttgcttgtg ctttttcttc tgcgctttgt
     5341 gccactgttt ttgctgatgt gagtccatca gcgccgcggc cagttcttct ttcatctcct
     5401 tatactgttc gctgagattg ttgacccgta actcgccacg ctggcgttta ttacgttgag
     5461 caacattgac aataatggcg gcaatcgccg caatcgctag cacaacggta acgattttcg
     5521 ccaaaaacaa accatattca gacaacaatt ccacgagtcc ccaccttggt taaacaacgc
     5581 tttggctcac agtttacaac aggcatgtta agccgtctcg cagagatgtt attgcagcga
     5641 aagtatgaca aagtatccat tttgcggtgt tatcgcccca ggctgattga aatgcccttc
     5701 tgtttcaggc ataaagcccc aaagtcataa agtacactgg cagcgcgaca gacaacgtcg
     5761 ttgaggagta gccatgcatt accagccaaa acaagattta ctcaatgatc gcattatcct
     5821 ggtgacggga gccagcgatg ggattggtcg tgaagccgcg atgacgtatg cacgctatgg
     5881 tgcgacagtg attctgttgg gccgtaatga agaaaaatta cgtcaggtag ccagccacat
     5941 aaacgaagaa actgggcgtc agccacagtg gtttattctc gatttgctga cctgcacgtc
     6001 cgaaaattgc caacaactgg cacagcgcat tgtcgttaat tatccgcgtc tggatggtgt
     6061 tttgcataat gccggattgc tcggcgatgt ttgcccaatg agcgaacaaa atccgcaggt
     6121 ctggcaggac gtcatgcagg tcaacgttaa tgccaccttt atgctcaccc aggcactgct
     6181 tcctttatta ctcaaatcgg acgccggttc actggtcttt acttcatcaa gcgttgggcg
     6241 tcagggacga gccaactggg gtgcatatgc agcgtcgaaa tttgccaccg aagggatgat
     6301 gcaggtactg gccgatgaat atcagcagcg cctgcgcgtc aactgcatta acccaggcgg
     6361 tacgcgcacc gcaatgcgcg caagcgcctt cccgaccgaa gatccacaga aacttaaaac
     6421 acccgctgat atcatgccgc tctacctctg gctgatgggc gatgacagcc gccgtaaaac
     6481 cggcatgacc tttgacgccc aaccgggccg taaaccagga atttcccaat gagtgatgaa
     6541 cgctaccaac agcgtcagca gcgagtgaaa gaaaaagtag atgctcgtgt ggcccaggcc
     6601 caggatgaac gcggtattat catcgtcttt accggcaatg gaaaaggcaa aaccaccgcg
     6661 gcatttggta cggcaacacg cgcagttggt cacggaaaaa aagtgggcgt cgtgcagttt
     6721 attaaaggca cctggcctaa tggcgaacgc aatctgctgg agccacatgg cgttgagttt
     6781 caggtgatgg caacgggctt tacctgggat acacaaaacc gcgagtctga taccgccgcc
     6841 tgccgcgaag tctggcaaca tgcaaagcgg atgcttgctg attcctcact ggatatggtt
     6901 ttgcttgatg aactgacgta tatggtggcg tatgactatt tgccactgga agaagtggtg
     6961 caggcgttaa atgaacgtcc acatcaacag acggtgatta tcacgggtcg aggctgtcat
     7021 cgggatattc ttgaactggc ggatacggta agtgagctgc gccccatcaa acatgcgttt
     7081 gatgccggtg ttaaggcgca aatagggatt gattattaat ctgtaaaaat gccggatatg
     7141 acgtccggca tactttgatt aaccgttatt acgcccgcca gaacgacgac cgccgctcgc
     7201 ctgactgtga cgtttcaccg cacgacgaat ctgattagcc ttcatgcgac gacggtcttt
     7261 ttctacagcg actttagagc tggtttctgg cggaagctcc accagttcgc gcagatagtt
     7321 agtctgggcg aggtccagct ccgtccagcc accgcgtggc agaccttttg gcagtgggat
     7381 atcaccgtaa cgaacacgga tcaggcggct cacctgcaca ccaaccgctt cccacagacg
     7441 acgaacttca cggttacggc cttcggtcag agtcacgttg taccactggt tgatcccttc
     7501 gccgccgctg aacttgatgg ttttaaaagc tgccggacca tcttccagtt gcacgccacg
     7561 actcaaatca cgcagtttcg cgtcgtcaac ctgaccaaat acacgcacgg cgtattcacg
     7621 ttcaacttca cggcttggat gcattaaacg gtttgccagt tcaccatcgg tggtgaacag
     7681 cagcagacca caggtgttaa cgtccagacg acctacggca atccagcgtg cgccacgcag
     7741 ctttggcaga cggtcaaaca ccgtcggacg cccttccgga tcgttacggg tacacaactc
     7801 accttccggt ttgtaatagg ccagaacgcg acaaatttgt tcagcggact cacgtaccga
     7861 aatcagatga ccatcgatac gaattttcag cccaggggta acttcaacgc gatcgccgag
     7921 tttagcaatt ttgccatcaa cactcacacg accggcttca ataatagatt cgatttcacg
     7981 gcgggagccg tggccagcgc gggccagcac tttctgtaat ttttcgctca tagagcttcc
     8041 ttttggtgtc gccttcccag gcgtctgata tgcttttgtg caacagtgag tcagaactta
     8101 catctgactc actgatagta aagtctgttt gaactcttca ggccttaagt aaacgactcg
     8161 acgttttaac tggcctggct ttgaggcgca acatactaca ctatctttat ggttaaagct
     8221 tgtttcgatt aacatcagct taattaccag ccaccaccgc ctccgccacc cgcgccgcca
     8281 ccagcaccac caccggaata acttcgcccc aacaaactgg ttttggcccc cagactcagg
     8341 tgcatctgta atgaaaacaa aacgccacta cgggcaattt ccggggcgct aacagccgct
     8401 gtgagtgatg gggtgatacg tgctacccat tctcttccaa ggtttgctga gactgcccaa
     8461 cctagcaacg cgtaatccag tttccgtgtt tcccctttcc gccgacgccc actgtgtgtt
     8521 gctgcctctt tacgcgcaag atagttaacg atagcttcac cgtgggcata acgttgctgc
     8581 ccagtttgtg taaactttgg cagaaaaata taccccatgc cagtgagata accggtcagg
     8641 aaaacagcgc taaaataacc cgctggcata taccagtaaa agaatacatc tccgacattg
     8701 atgtagagag aatagaaggc cacgcctgaa caaatcagtg gtaaacaatt cttccgtcct
     8761 caaggatgcg gccatttcga tatgtgtcgc cctcttctcc agtaaagaga tcgatttcgc
     8821 taaatggatt acccgcgata tcagggaact tgagtgaaaa tcgggtctta tagatttcaa
     8881 acggccagtg gttaccagtc acgttccaga ttaacagatc tgaatctccc tcacgcagga
     8941 aagcattttt aacctgataa tgaatttgat aatgatagtc gccttgaggc aagaaacgtt
     9001 gtttatcgcc cacgacaata ctcattaacc cgatatgcca gtcatgatgc cagggttcag
     9061 gaataccatc acgggtgacg ccaacaatat gatagttcag cagtgcagcg tccccaccag
     9121 gctgcatcca gagtcgtggt aaatcacgat aaaatccgtg tttaatctgc tgatttcgcg
     9181 cctgaaaatg aatattttct ttgatgtcga gcaccccatc cggttgcaga gtggcttcaa
     9241 catcaaagag cgaaatccct tcttcataag aagggatctc cgtgttttct gcataactta
     9301 gagatgatac aaatagccct gctatcaaca gcaaaatgca gcgaaacttc cctgccatag
     9361 tcttccatcc gtggaacgca atttgttata agaaaggctt cacatcacct acgccttcac
     9421 gcaccacgac cggcgtatca tcggtaagat caataaccgt tgtcggtttc tgcccgagat
     9481 agccgccgtg aataatcaaa tctacctgtt tttccagacg gtctttaatt tcttcgggat
     9541 cggattcggt aaattcgctg cctggcagca ttagcgaagt ggaaagcatc ggttcgccca
     9601 gtgcttcaag caacgcctgc gcaatcgggt tagaaggcac acgcataccg atggttttgc
     9661 gtttttcctg caacagacgg cgtggcactt ccttcgtccc cttcaggatg aaggtatagt
     9721 tgcccggcgt gttgtttttc attaaacgaa acgcaacgtt atcaacaaat gaataggtcg
     9781 agagttcaga aagatcgcga cacatcaggg taaagttgtg accgtccggc agctggcgaa
     9841 tacgacaaat acgctccatc gcgtttttat cttcaatttt acagccgagc gcatagccgg
     9901 aatcagttgg ataaacaatc accccgcctt tacgcacgat ctccaccgcc tggttgatca
     9961 gacgttgctg tgggttatca ggatgaatat aaaaaaactg gctcataatt ccctctctgt
    10021 ggtgttctgc ggctgttccc atagctgcca gacgccttca acgcctgcgg gcagccagag
    10081 tttacgacct agttcaatcc acgggcatgg ctgatgaaaa tcagatcctt gtgatgccca
    10141 taaatgatgc tgacgcgcaa gggccgccag ctgggtacgt tcattgggcg attgctggca
    10201 ctgcgcgact tccatcgcgt caccgtggtg ttcggcaaaa tgcgcaacca atcttttcag
    10261 ccatttagca ctaagattgt accgcccagg atgagctagc accgccttac cgccagaatg
    10321 atgaatgaca tcaatagctt gttctattgt acaccactgt ggcggaacgt atccggtttt
    10381 cccgcgcgcc agatattttt taaagacatc cgccattgaa ctggctttgc cgcaccccac
    10441 aaggaaacgc gcaaaatgac cgcgcgtcac cgcaccgccc tgcgccagtc gtcgcgcgcc
    10501 ttccagtgcc cctggtattt gcgctttttc aagtctttcg gcaatcagtt gcgcccgctg
    10561 attgcgccgt tctgtctgct gcgcgaggaa ctcacacatt agcggatgag taatatcaat
    10621 attcagcccg acaatatgaa tttcatgatt ttcccagacc gtggaaattt ccacgccggg
    10681 aataagattc agcgccagtc ccgaacgtga aatttcctct cttgctggca cgattgcagc
    10741 tgtgttgtca tggtcggtga tcgccagggt accgacgcgc atctcgactg cacggtgcac
    10801 caatacttct ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat
    10861 cactgcgtaa ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg
    10921 acatcataac agttctggca aatattctga aatcagctgt tgacaattaa tcatcgaact
    10981 agttaactag tgcgcaagtt cacgtaaaaa gggtatcgac aatgaaagca attttcgtac
    11041 tgaaagcttg gtggcgcact tcctgaaacg ggcagtgtat tcaccatgcg taaagcaatc
    11101 agatacccag cccgcctaat gagcgggctt ttttttgaac aaaattagag aataacaatg
    11161 caaacacaaa aaccgactct cgaactgcta acctgcgaag gcgcttatcg cgacaacccg
    11221 actgcgcttt ttcaccagtt gtgtgggaat cgtccggcaa cgctgctgct ggaatccgca
    11281 gatatcgaca gcaaagatga tttaaaaagc ctgctactgg tagacagtgc gctgcgcatt
    11341 acagcattag gtgacactgt cacaattcag gcactttccg gcaacggcga agccctgctg
    11401 gcactactgg ataacgccct gcctgcgggt gtggaaaatg aacaatcacc aaactgccgt
    11461 gtgctgcgct tcccccctgt cagtccactg ctggatgaag acgcccgctt atgctccctt
    11521 tcggtttttg acgctttccg tttattgcag aatctgttga atgtaccgaa ggaagaacga
    11581 gaagcaatgt tcttcggcgg cctgttctct tatgaccttg tggcgggatt tgaagattta
    11641 ccgcaactgt cagcggaaaa taactgccct gatttctgtt tttatctcgc tgaaacgctg
    11701 atggtgattg accatcagaa aaaaaacacc cgcattcagg ccagcctgtt tgctccgaat
    11761 gaagaagaaa aacaacgtct cactgctcgc ctgaacgatc ttcgccagca gctgaccgaa
    11821 gccgcgccgc cgctgccggt ggtttccgtg ccgcatatgc gttgtgaatg taatcagagc
    11881 gatgaagagt tcggtggcgt agtgcgtttg ttgcaaaaag cgattcgcgc tggagaaatt
    11941 ttccaggtgg tgccatctcg ccgtttctct ctgccctgcc cgtcaccgct ggcggcctat
    12001 tacgtgctga aaaagagtaa tcccagcccg tacatgtttt ttatgcagga taatgatttc
    12061 accctgtttg gcgcgtcgcc ggaaagttcg ctcaagtatg acgccaccag ccgccagatt
    12121 gagatctacc cgattgccgg aacacgtcca cgcggtcgtc gtgccgatgg ctcgctggac
    12181 agagacctcg acagccgcat cgaactggaa atgcgtaccg atcataaaga gctttctgaa
    12241 catctgatgc tggtggatct cgcccgtaat gatctggcac gcatttgcac ccccggcagc
    12301 agctacgtcg ccgatcttac caaagttgac cgttactctt acgtgatgca cctggtctcc
    12361 cgcgtggtcg gtgagctgcg ccacgatctc gacgccctgc acgcttaccg cgcctgtatg
    12421 aatatgggga cgttaagcgg tgcgccgaaa gtacgcgcca tgcagttaat tgccgaggcg
    12481 gaaggtcgtc gccgcggcag ctacggcggc gcggtaggtt atttcaccgc gcatggcgat
    12541 ctcgacacct gcattgtgat ccgctcggcg ctggtggaaa acggtatcgc caccgtgcaa
    12601 gccggtgctg gcgtagtcct tgattctatt ccgcagtcgg aagccgacga aacccgtaat
    12661 aaagcccgcg ctgtactgcg cgctattgcc accgcgcatc atgcacagga gactttctga
    12721 tggctgacat tctgctgctc gataatatcg actcttttac gtacaacctg gcagatcagt
    12781 tgcgcagcaa tgggcataat gtggtgattt accgcaacca tattccggcg cagaccttaa
    12841 ttgaacgcct ggcgacgatg agcaatccga tactgatgct ttctcctggc cccggtgtgc
    12901 cgagcgaagc cggttgtatg ccggaactcc tcacccgctt gcgtggcaag ctgcccatta
    12961 ttggcatttg cctcggacat caggcaattg tcgaagctta cgggggctat gtcggtcagg
    13021 cgggcgaaat tcttcacggt aaagcgtcga gcattgaaca tgacggtcag gcgatgtttg
    13081 ccggattaac aaacccgctg ccggtggcgc gttatcactc gctggttggc agtaacattc
    13141 cggccggttt aaccatcaac gcccatttta atggcatggt gatggcagta cgtcacgatg
    13201 cggatcgcgt ttgtggattc cagttccatc cggaatccat tctcaccacc cagggcgctc
    13261 gcctgctgga acaaacgctg gcctgggcgc agcagaaact agagccagcc aacacgctgc
    13321 aaccgattct ggaaaaactg tatcaggcgc agacgcttag ccaacaagaa agccaccagc
    13381 tgttttcagc ggtggtgcgt ggcgagctga agccggaaca actggcggcg gcgctggtga
    13441 gcatgaaaat tcgcggtgag cacccgaacg agatcgccgg ggcagcaacc gcgctactgg
    13501 aaaacgcagc gccgttcccg cgcccagatt atctgtttgc tgatatcgtc ggtactggcg
    13561 gtgacggcag caacagtatc aatatttcta ccgccagtgc gtttgtcgcc gctgcctgtg
    13621 ggctgaaagt ggcgaaacac ggcaaccgta gcgtctccag taaatctggt tcgtccgatc
    13681 tgctggcggc gttcggtatt aatcttgata tgaacgccga taaatcgcgc caggcgctgg
    13741 atgagttagg tgtttgtttc ctctttgcgc cgaagtatca caccggattc cgccacgcga
    13801 tgccggttcg ccagcaactg aaaacccgca ccctgttcaa tgtgctgggg ccattgatta
    13861 acccggcgca tccgccgctg gcgttaattg gtgtttatag tccggaactg gtgctgccga
    13921 ttgccgaaac cttgcgcgtg ctggggtatc aacgcgcggc ggtggtgcac agcggcggga
    13981 tggatgaagt ttcattacac gcgccgacaa tcgttgccga gctgcatgac ggcgaaatta
    14041 agagctatca attgaccgct gaagattttg gcctgactcc ctaccaccag gagcaactgg
    14101 caggcggaac accggaagaa aaccgtgaca ttttaacacg cttgttacaa ggtaaaggcg
    14161 acgccgccca tgaagcagcc gtcgctgcga acgtcgccat gttaatgcgc ctgcatggcc
    14221 atgaagatct gcaagccaat gcgcaaaccg ttcttgaggt actgcgcagt ggttccgctt
    14281 acgacagagt taccgcgctg gcggcacgag ggtaaatgat gcaaaccgtt ttagcgaaaa
    14341 tcgtcgcaga caaggcgatt tgggtagaaa cccgcaaaca gcagcaaccg ctggccagtt
    14401 ttcagaatga ggttcagccg agcacgcgac atttttatga tgcgctacag ggtgcacgca
    14461 cggcgtttat tctggagtgc aagaaagcgt cgccgtcaaa aggcgtgatc cgtgatgatt
    14521 tcgatccggc acgcattgcc tccatttata aacattacgc ttcggcaatt tcggtgctga
    14581 ctgatgagaa atattttcag gggagctttg atttcctccc catcgtcagc caaatcgccc
    14641 cgcagccgat tttatgtaaa gacttcatta tcgaccctta ccagatctat ctggcgcgct
    14701 attaccaggc cgatgcctgc ttattaatgc tttcagtact ggatgacgac caatatcgcc
    14761 agcttgcagc cgtcgctcac agtctggaga tgggggtgct gaccgaagtc agtaatgaag
    14821 aggaactgga gcgcgccatt gcattggggg caaaggtcgt tggcatcaac aaccgcgatc
    14881 tgcgtgattt gtcgattgat ctcaaccgta cccgcgagct tgcgccgaaa ctggggcaca
    14941 acgtgacggt aatcagcgaa tccggcatca atacttacgc tcaggtgcgc gagttaagcc
    15001 acttcgctaa cggttttctg attggttcgg cgttgatggc ccatgacgat ttgcacgccg
    15061 ccgtgcgccg ggtgttgctg ggtgagaata aagtatgtgg tctgacgcgt gggcaagatg
    15121 ctaaagcagc ttatgacgcg ggcgcgattt acggtgggtt gattttggtt gcgacatcac
    15181 cgcgttgcgt caacgttgaa caggcgcagg aagtgatggc tgcggcaccg ttgcagtatg
    15241 ttggcgtgtt ccgcaatcac gatattgccg atgtggtgga caaagctaag gtgttatcgc
    15301 tggcggcagt gcaactgcat ggtaatgaag atcagctgta tatcgatacg ctgcgtgaag
    15361 ctctgccagc acacgttgcc atctggaaag cattaagcgt cggtgaaacc ctgcccgccc
    15421 gcgagcttca gcacgttgat aaatatgttt tagacaacgg ccagggtgga agcgggcaac
    15481 gtttcgactg gtcactatta aatggtcaat cgcttggcaa cgttctgctg gcggggggct
    15541 taggcgcaga taactgcgtg gaagcggcac aaaccggctg cgccggactt gattttaatt
    15601 ctgctgtaga gtcgcaaccg ggcatcaaag acgcacgtct tttggcctcg gttttccaga
    15661 cgctgcgcgc atattaagga aaggaacaat gacaacatta cttaacccct attttggtga
    15721 gtttggcggc atgtacgtgc cacaaatcct gatgcctgct ctgcgccagc tggaagaagc
    15781 ttttgtcagc gcgcaaaaag atcctgaatt tcaggcccag ttcaacgacc tactgaaaaa
    15841 ctatgccggg cgtccaaccg cactgaccaa atgccagaac attacggctg gcacgaacac
    15901 cacgctgtat ctgaagcgcg aagatttgct gcacggcggc gcgcataaaa ctaaccaggt
    15961 gctcggtcag gctttactgg cgaagcggat gggtaaaacc gaaatcatcg ccgaaaccgg
    16021 tgccggtcag catggcgtgg cgtcggccct tgccagcgcc ctgctcggcc tgaaatgccg
    16081 tatttatatg ggtgccaaag acgttgaacg ccagtcgcct aacgtttttc gtatgcgctt
    16141 aatgggtgcg gaagtgatcc cggtgcatag cggttccgcg acgctgaaag atgcctgtaa
    16201 cgaggcgctg cgcgactggt ccggtagtta cgaaaccgcg cactatatgc tgggcaccgc
    16261 agctggcccg catccttatc cgaccattgt gcgtgagttt cagcggatga ttggcgaaga
    16321 aaccaaagcg cagattctgg aaagagaagg tcgcctgccg gatgccgtta tcgcctgtgt
    16381 tggcggcggt tcgaatgcca tcggcatgtt tgctgatttc atcaatgaaa ccaacgtcgg
    16441 cctgattggt gtggagccag gtggtcacgg tatcgaaact ggcgagcacg gcgcaccgct
    16501 aaaacatggt cgcgtgggta tctatttcgg tatgaaagcg ccgatgatgc aaaccgaaga
    16561 cgggcagatt gaagaatctt actccatctc cgccggattg gatttcccgt ctgtcggccc
    16621 acaacacgcg tatcttaaca gcactggacg cgctgattac gtgtctatta ccgatgatga
    16681 agcccttgaa gccttcaaga cgctgtgcct acacgaaggg atcatcccgg cgctggaatc
    16741 ctcccacgcc ctggcccatg cgctgaaaat gatgcgcgaa aacccggaaa aagagcagct
    16801 actggtggtt aacctttccg gtcgcggcga taaagacatc ttcaccgttc acgatatttt
    16861 gaaagcacga ggggaaatct gatggaacgc tacgaatctc tgtttgccca gttgaaggag
    16921 cgcaaagaag gcgcattcgt tcctttcgtc acgctcgggg atccgggcat tgagcagtca
    16981 ttgaaaatta tcgatacgct aattgaagcc ggtgctgacg cgctggagtt aggcatcccc
    17041 ttctccgacc cactggcaga tggcccgacg attcaaaacg ccactctgcg cgcctttgcg
    17101 gcaggtgtga ctccggcaca atgttttgaa atgctggcac tgattcgcca gaaacacccg
    17161 accattccca ttggcctgtt gatgtatgcc aatctggtgt ttaacaaagg cattgatgag
    17221 ttttatgccc agtgcgagaa agtcggcgtc gattcggtgc tggttgccga tgtgccaatt
    17281 gaagagtccg cgcccttccg ccaggccgcg ttgcgccata atgtcgcacc tatcttcatc
    17341 tgcccgccaa atgccgatga cgacctgctg cgccagatag cctcttacgg tcgtggttac
    17401 acctatttgc tgtcacgagc gggcgtgacc ggcgcagaaa accgcgccgc gttacccctc
    17461 aatcatctgg ttgcgaagct gaaagagtac aacgctgcac ctccattgca ggggt
//
LOCUS       DAJVQG010000083        17449 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJVQG010000083 DAJVQG010000000
VERSION     DAJVQG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17449
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.083"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..823)
                     /gene="ydiK"
                     /locus_tag="OQM83_002482"
     CDS             complement(<1..823)
                     /gene="ydiK"
                     /locus_tag="OQM83_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCS1520723.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGD"
     gene            1212..4268
                     /locus_tag="OQM83_002483"
     CDS             1212..4268
                     /locus_tag="OQM83_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCS1520724.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4265..4675
                     /gene="menI"
                     /locus_tag="OQM83_002484"
     CDS             4265..4675
                     /gene="menI"
                     /locus_tag="OQM83_002484"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCS1520725.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            4775..4963
                     /locus_tag="OQM83_002485"
     CDS             4775..4963
                     /locus_tag="OQM83_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCS1520726.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            5511..5879
                     /gene="sufA"
                     /locus_tag="OQM83_002486"
     CDS             5511..5879
                     /gene="sufA"
                     /locus_tag="OQM83_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCS1520727.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            5888..7375
                     /gene="sufB"
                     /locus_tag="OQM83_002487"
     CDS             5888..7375
                     /gene="sufB"
                     /locus_tag="OQM83_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCS1520728.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            7385..8131
                     /gene="sufC"
                     /locus_tag="OQM83_002488"
     CDS             7385..8131
                     /gene="sufC"
                     /locus_tag="OQM83_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCS1520729.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8106..9377
                     /gene="sufD"
                     /locus_tag="OQM83_002489"
     CDS             8106..9377
                     /gene="sufD"
                     /locus_tag="OQM83_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCS1520730.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            9374..10594
                     /gene="sufS"
                     /locus_tag="OQM83_002490"
     CDS             9374..10594
                     /gene="sufS"
                     /locus_tag="OQM83_002490"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCS1520731.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            10607..11023
                     /gene="sufE"
                     /locus_tag="OQM83_002491"
     CDS             10607..11023
                     /gene="sufE"
                     /locus_tag="OQM83_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCS1520732.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11172..12176
                     /gene="ldtE"
                     /locus_tag="OQM83_002492"
     CDS             11172..12176
                     /gene="ldtE"
                     /locus_tag="OQM83_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCS1520733.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12239..12475)
                     /gene="lpp"
                     /locus_tag="OQM83_002493"
     CDS             complement(12239..12475)
                     /gene="lpp"
                     /locus_tag="OQM83_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCS1520734.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(12785..14197)
                     /gene="pykF"
                     /locus_tag="OQM83_002494"
     CDS             complement(12785..14197)
                     /gene="pykF"
                     /locus_tag="OQM83_002494"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCS1520735.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            14754..14963
                     /gene="fumD"
                     /locus_tag="OQM83_002495"
     CDS             14754..14963
                     /gene="fumD"
                     /locus_tag="OQM83_002495"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCS1520736.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            15419..16045
                     /locus_tag="OQM83_002496"
     CDS             15419..16045
                     /locus_tag="OQM83_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCS1520737.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            16066..>17449
                     /locus_tag="OQM83_002497"
     CDS             16066..>17449
                     /locus_tag="OQM83_002497"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCS1520738.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKIKDFYYRLAHRVGELSHLADGSYAIAER
                     WNLGEEYWGYA"
BASE COUNT         4439 a         4120 c         4603 g         4287 t
ORIGIN      
        1 tatcgccggt ccagtagagc cagataatcg ccggaatcag taccggcaac gggccaagct
       61 ggacaaggca ggagaggatc attaacaccg ttagcaacgt tgcataaggt acgccggata
      121 cggcgaggcc gatactgcca agcactgcct gtactaacgc cgtcaccacc acacccagcg
      181 ccaccgcgcg gatagcctgt gccgccagca gcacggcggc atctccgcga acgcctgcca
      241 gacgggttgc aaaatggcga atgccttgtg ccacctgttc accgcgccag tacagcaggg
      301 cactgaagag aagcatcagc gcacaatgca ccataaagcg cccgatatgc gccgcctgcc
      361 caacgaacca ggtggtggtg gtgccaatat aagggcggac tttcgccatg atcgccgtac
      421 cccccatatc cagcaagttg tgccagcctg catacagctt cgcgccaatc accggaatgg
      481 tattaagcca cgccagatcg ggtaacgtca tgtcgccgct ggaaatggct ttaattagcg
      541 ggccgctgcc gtcgacgata ctgttaacca gcaaagcaat agggatgata aacaccatca
      601 ctaataacag cgtcatcacc agaacggcga gagagcggcg gccaaacatg atcttttgca
      661 aacgtaacaa taccggccag gtggcgataa ccaccgtacc ggcccatgca aagccgagaa
      721 taaagggttg aacaatccac agacatgcca caatcatgat ggctaaaaac agcaccgaaa
      781 gcagaatttg tgcgacatcc ctgggctgac gaacatttac cataactact tttcaccttt
      841 tttgtgcgtc aaatcgtcga cgcgacgtga acacgcgaaa ctcacctgca taatgatgag
      901 caatttcagc ggtttttaac aggccgattc tgcccttaat tctgatgggc gtgttagaaa
      961 aaaatgtggt acaacaactc aggcaacacg caaacgattt actcgtcgta tttcaagttg
     1021 catgcgctgc ggctacgttc gttcacccca gtcacttact attgtaagct cctggagttc
     1081 actcgcttgc cgccttcctg caactcgaaa tccatagagt aaataaacat cacctttcag
     1141 tgcgctattg cagccgcctg aaagaatagt gtatcgacct acatcacaga ccgcaggaaa
     1201 gggtcaatat aatgattcca cagatttccc aggcacccgg cgtcgttcaa ctggtgctta
     1261 attttttgca agagctggag caacaaggtt ttaccggcga tacggcgaca agttatgccg
     1321 atcgtctgac aatgtcgacc gacaacagta tttaccaact tctccccgat gcggtggtat
     1381 ttccgcgttc aaccgcagat gtggcgctga tcgcccgtct tgccgcgcag gaacgctatt
     1441 catcgctgat ctttaccccc cgcggcggcg gcaccggcac taacggtcag gcgctcaacc
     1501 aggggattat tgttgatatg tcccgccata tgaaccgcat catcgaaatt aaccctgaag
     1561 agggctgggt gcgcgttgag gccggggtga taaaagatca actcaatcag tacctgaaac
     1621 cgttcggcta cttttttgca ccagaacttt cgaccagcaa ccgggcaacg ctcggcggga
     1681 tgatcaatac cgatgcatcc ggtcagggat cgctggtcta tggcaaaacg tcagatcacg
     1741 tacttggcgt acgcgcggtg ttgttggggg gcgatattct cgatacgcaa cctttacccg
     1801 tcgaactggc ggaaacgctg ggtaaaacca ataccacaat cgggcgaatt tataacacgg
     1861 tttatcaacg ttgccgtctg caacgccagt taatcatcga caacttcccc aaacttaacc
     1921 gctttcttac cggttacgat ctgcgtcatg tctttaacga tgagatgacc gagttcgacc
     1981 tgacgcgcat tctgacgggt tcagaaggga cgctggcctt tattaccgaa gcgcggctgg
     2041 atattacgcc cttgcctaaa gtgcgccgtc tggtgaacgt caaatatgac tcttttgact
     2101 ccgcgctgcg taacgcgccg tttatggttg aggcgcgggc gctttcggta gagacggtgg
     2161 actcaaaagt gctgaatctg gcgcgggaag atattgtctg gcattccgtc agcgagttga
     2221 ttaccgatgt gcctgacaaa gagatgctcg ggctgaacat tgtggaattt gctggtgatg
     2281 atgaggcgct gattgacgag cgagttaatg cactctgtgt gcggcttgat gagctgattg
     2341 ccagccaaca ggcaggtgtg attggctggc aggtgtgccg cgagctggcg ggggttgaac
     2401 gtatctatgc aatgcgcaaa aaagccgttg gtctgcttgg caatgccaaa ggtgccgcta
     2461 agccaattcc gtttgctgag gatacctgcg taccgccgga acatctggcc gattatattg
     2521 ctgaatttcg cgcgctgctc gacagccacg gcttaagcta cggtatgttc ggtcacgtcg
     2581 acgcaggtgt tttgcacgtc cgtccggcac tggatatgtg cgatccgcaa caagagattt
     2641 tgatgaagca aatctctgat gacgtggtgg cgctgactgc gaaatacggt ggtttgttgt
     2701 ggggcgagca cggcaaaggt tttcgcgctg aatacagccc ggcgtttttc ggtgaggaac
     2761 tttttgcaga actgcgcaaa gtgaaagcgg catttgaccc gcataaccga ctcaacccag
     2821 ggaagatttg cccgccagaa ggtctcgatg cgccgatgat gaaagtggac gcggtgaagc
     2881 gcggtacatt cgatcggcag atccccattg cggtacgcca gcagtggcgc ggtgcgatgg
     2941 agtgtaacgg caacggttta tgcttcaact ttgatgcccg tagtccgatg tgtccgtcga
     3001 tgaagatcac ccagaaccgg attcattcac cgaaagggcg cgcaacgctg gtgcgtgaat
     3061 ggctgcgttt gttggcggat cgcggcgttg atccactcaa actggaacaa gaactgcctg
     3121 aatccggcgt cagtttacgg acgttaattg cccgcacgcg caatagctgg catgcgaata
     3181 aaggcgaata tgacttctca cacgaagtca aagaggcgat gtcgggctgt ctggcctgta
     3241 aagcgtgttc gacccagtgc cccatcaaaa ttgatgtgcc ggagtttcgc tctcgtttcc
     3301 tgcagctcta tcacacccgt tatttacgcc cgctgcgcga ccacctcgtc gctacggtcg
     3361 agagctacgc accgctgatg gcacgcgcgc cgaaaacctt taacttcttc attaaccagc
     3421 cgctggtgcg caaactctcg gaaaaacata tcggcatggt tgatttgcca ctgctgtcgg
     3481 tcccctcgct acaacaacaa atggtggggc atcgctcggc aaacatgacg ctggaacagc
     3541 ttgaagctct caatgcagag cagaaagcgc gcacggtgtt ggtggtgcag gaccccttta
     3601 ccagctatta cgatgcgcaa gtggtggcgg attttgtccg tctggttgaa aaattaggtt
     3661 tccagcctgt gttactgcca ttttcgccaa atggcaaagc ccagcatatc aaaggctttc
     3721 ttaatcgttt tgcgaagacg gcgaaaaaga cggcggattt cctcaaccgt atggctaagc
     3781 ttgggatgcc aatggtgggc gtcgatccgg cgctggtact ttgttatcgc gatgaatata
     3841 aactggcgct gggcgaggaa cgtggcgagt ttaacgtctt acttgcgaat gaatggctgg
     3901 caagcgcact tgagtcacat ccagtggcta cagtcagcgg tgaatcatgg tatttctttg
     3961 gtcactgtac cgaggttacc gctttgccgg gcgcgccagc acaatgggcc gcgatatttg
     4021 cccgttttgg cgcgaaactg gaaaatgtca gcgtgggttg ctgcggcatg gcagggactt
     4081 acggacatga agcgaaaaac catgaaaatt cgctcgggat ctatgagtta tcctggcatc
     4141 aggctatgca gcgactgccg cgtaaccgct gtctggcgac cggatattcc tgccgtagcc
     4201 aggtaaaacg ggttgaaggc acgggggtac gccatcctgt gcaggcttta ctggagatta
     4261 ttaaatgata tggaaacgga aaatcaccct ggaagcactg aatgctatgg gtgaaggaaa
     4321 catggtgggg ttcctggata ttcgctttga acatattggt gatgacaccc ttgaagcgac
     4381 aatgccagta gactcgcgga caaagcagcc tttcgggttg ctgcatggag gagcatccgt
     4441 ggtactggcc gaaagtatcg gttccgttgc cggttattta tgtaccgaag gtgagcaaaa
     4501 agtggttggt ctggaaatca atgctaatca cgtccgctcg gcacgagaag ggcgggtgcg
     4561 cggcatatgt aaaccgttgc atctcggttc gcgtcaccag gtctggcaga ttgaaatctt
     4621 cgatgagaaa gggcgtttgt gctgttcgtc acgattgacg accgccattt tgtgacgttg
     4681 tcaccagaaa agtgtgacgc atattgcttt tggtcattat ctgcacagta aacggtggta
     4741 tactgtgcac gtcttgtcta aaagcgagga tgctatgtct actcaacttg atcccaccca
     4801 gctggccatt gaatttttac gccgtgacca gagcaatctt tcacctgccc aatatctcaa
     4861 acgtctgaaa caactagaac ttgaatttgc tgacctgctt actctctctt ctactgaatt
     4921 aaaagaagaa atctactttg cctggcgtct gggcgtgcat tgatcctgac ttaatgccgc
     4981 aagttcgcca aatcgacctg gattaacctc cgtccaggtt cgatgacctt cttaatactg
     5041 cttatctacc gcctgatatt gttcaaaaat aatactaatc gcagtttgtg ttaaaacggc
     5101 gggttagctt tatgagatta ttcttatcgc cccttcaaga gctaagccac tgagagtgcc
     5161 ggagataagc gccggatggg gtagaaaccc ttaagcctgt gtcgcacaga cttaagggtt
     5221 ttcttatttc tgaacgctgc acttttttac ctatctctta aatagtcatt ttcaatacaa
     5281 ctttttcttt ttcatcacag ccttaatgct ccttagataa taaccattat ctaacaatga
     5341 gatacctaat tcttagcgtg cctgttaacc cacaaatcag ggtctatgct tattaaaagc
     5401 aggcaaaaag catgttttac ggtaaagccc ctgcgtttgc tgggttgaac tgataatcat
     5461 tatcactagc atgctgttat acgctgaaag cgatgaagtg aggtaaatcg atggacatgc
     5521 attcaggaac ctttaaccca caagatttcg cctggcaagg cttaacgctg acacccgcag
     5581 cggcgataca catccgtgag ctggtggcaa agcagccggg tatggtcggc gtgcgcttag
     5641 gcgtgaagca aacgggctgc gcgggctttg gctatgtgct cgacagtgtt agcgagccgg
     5701 acaaagacga tctgctgttt gaacacgacg gcgcgaagct gtttgtcccg ctgcaagcga
     5761 tgccgtttat tgatggcacg gaagtcgatt tcgttcgtga aggacttaat cagatattca
     5821 aatttcacaa ccctaaagcc cagaatgaat gtggctgtgg cgaaagcttt ggggtatagg
     5881 cggtactatg tctcgtaata ctgaagcaac tgacgatgtc aaaacctgga ccggcggccc
     5941 gctgaattat aaagaaggat tcttcaccca gttagccacc gatgagctgg caaaggggat
     6001 aaacgaagag gtggtgcgcg caatttcggc gaagcgtaat gagccggagt ggatgctgga
     6061 gtttcgtcta aacgcctatc gcgcatggct ggagatggaa gaaccgcact ggttgaaagc
     6121 gcactacgac aagctgaatt atcaggatta cagttactac tcagcaccat cgtgcggtaa
     6181 ttgtgacgac acttgcgcgt ctgaacctgg cgcggtgcag caaactggcg cgaacgcctt
     6241 tttaagtaaa gaggtggagg cggcgtttga gcagttgggc gttcccgtgc gggaaggcaa
     6301 agaggtggcg gtggatgcca ttttcgactc tgtttcggtt gccactactt atcgcgaaaa
     6361 actggcggag cagggaatta ttttctgttc ctttggtgag gcgatccacg atcatccgga
     6421 actggtgcgt aaatatctcg gcactgtggt gccgggaaat gacaacttct ttgccgcgct
     6481 taatgcggcg gtagcctctg acggtacgtt tatttatgtg cctaaaggcg tgcgctgccc
     6541 gatggaactt tccacctatt ttcgcattaa cgcggaaaaa accgggcagt ttgagcgcac
     6601 cattctggtg gccgacgaag acagctacgt cagctacatt gaaggctgtt ccgctccggt
     6661 gcgtgacagc tatcagttac acgcggcggt ggtggaagtc atcatccata aaaacgccga
     6721 ggtgaaatat tccacggtac aaaactggtt ccctggcgat aacaacaccg gcggtattct
     6781 caatttcgtc accaagcgtg ctttgtgcga aggcgaaaac agcaaaatgt catggacgca
     6841 atcagaaacc gggtcagcga ttacgtggaa atatcccagc tgcattttgc gcggcgataa
     6901 ctccattggt gagttttact cagtggcgct gaccagcggt catcagcaag cggataccgg
     6961 caccaagatg atccacatcg gtaaaaacac caaatcgacc attatctcga aagggatctc
     7021 tgccggacat agtcagaaca gttatcgcgg cttagtgaaa atcatgccga cggcaaccaa
     7081 tgcgcgcaat ttcactcagt gcgactcaat gctgattggc gctaattgtg gggcgcatac
     7141 cttcccgtat gtcgaatgtc gcaataacag cgcacaactg gagcacgagg caacgacatc
     7201 acgtattggt gaagatcaac tgttttactg cctgcaacgc gggattagcg aagaagacgc
     7261 catctcgatg attgttaacg gtttctgcaa agacgtgttc tcagaactgc cgctggaatt
     7321 tgccgttgaa gcacaaaaac tcctcgccat cagtcttgaa cacagcgtcg gataaggaat
     7381 aaacatgtta agtattaaag atttacacgt cagcgtggaa gataaagcta tcctgcgtgg
     7441 attaagcctc gacgttcgtc ccggcgaagt tcacgccatt atggggccaa acggttcggg
     7501 caaaagtacc ttatcggcaa cgcttgccgg gcgagaagat tatgaagtga cgggcggcac
     7561 ggttgagttc aaaggcaaag atttgcttgc gctgtcgccg gaagatcgtg caggcgaagg
     7621 catctttatg gccttccagt atccggtgga gattcccggc gtcagtaacc agtttttcct
     7681 gcaaacggca cttaatgcgg tgcgcagcta tcgcggccag gaaacgctcg accgctttga
     7741 ttttcaggat ttgatggaag agaaaatcgc tctcctgaag atgccggaag atttattaac
     7801 ccgttcggta aacgttggtt tttccggcgg cgagaaaaag cgcaacgata ttttgcaaat
     7861 ggcggtgctg gaaccggagt tatgcattct tgatgaatcg gactccgggc tggatattga
     7921 cgcattaaaa gtggttgccg atggcgtgaa ctcgctgcgt gacggcaagc gctcattcat
     7981 cattgttacg cactaccaac gcattctcga ctacatcaag cctgattacg tccatgtgct
     8041 atatcaggga cgaattgtga aatccggcga tttcacgttg gtcaaacaac tggaggagca
     8101 gggttatggc tggcttaccg aacagcagta atgcgctgca acagtggcat cacttgtttg
     8161 aagctgaagg gacaaaacgc tcaccgcaag cacagcagca tttacaacaa ttgctgcgta
     8221 ccggactgcc gacacgtaaa catgaaaact ggaaatatac gccgctggaa gggctgacca
     8281 atagtcagtt tgtcagcatt gcgggagata tatccccaca gcagcgtgat gccttagcgt
     8341 taacgttaga cgccgtgcgg ctggtgtttg tcgatggacg ttacgtgccc gcgctgagcg
     8401 atgcaattga aggcagcgga tatgaagtga gcattaacga cgaccgtcag gggttacccg
     8461 acgctattca ggcggaagtg tttctgcatt tgacggaaag cctggcacaa agcgtgacgc
     8521 atatcgccgt gaagcgcggt caacggccgg caaagccatt gctgttaatg catatcaccc
     8581 agggcgtggc aggtgaagag gtgaacactg cccattaccg gcatcatctg gatctggcgg
     8641 aaggtgccga agcaacggtg atcgaacatt ttgtcagcct taatgatgct cgtcatttta
     8701 ccggggcacg gttcactatc aatgtcgcag cgaacgccca cttgcagcat atcaagctgg
     8761 cgtttgaaaa cccgctcagt caccactttg ctcataacga tttgttgctg gctgaggatg
     8821 ccaccgcatt tagccatagt ttcctgctgg gtggcgcagt gttacgacac aacaccagta
     8881 cgcaactcaa tggcgaaaac agcacgctgc ggatcaatag cctggcgatg ccggtgaaaa
     8941 acgaggtgtg tgatacccgt acctggctgg aacacaataa aggtttttgt aacagccgac
     9001 agttgcacaa aactatcgtc agcgacaaag gccgcgcggt atttaacggt ttgatcaacg
     9061 tcgcgcagca cgccatcaaa acggatggtc agatgaccaa caataatctg ctgatgggca
     9121 aaatggcgga agtggataca aaaccgcagc tggaaatcta tgcagatgat gtgaaatgca
     9181 gccacggcgc gacggtgggg cgtattgatg atgaacagat gttctatctg cgctcgcgcg
     9241 ggatcaatca gcaggatgcc cagcagatga tcatttacgc ctttgctgcc gaactgacgg
     9301 aagcactgcg tgatgagggg cttaaacagc aggtgctggc ccgaatcggt caacggctgc
     9361 caggaggtgc aagatgactt tttccgtcga caaagtgcgg gccgactttc cggtgctttc
     9421 tcgtgaggtg aacggtttgc cgctggctta tctcgatagc gccgccagtg cgcagaaacc
     9481 aagccaggtg attgacgccg aggctgagtt ttatcgtcac ggctacgcgg cggtgcatcg
     9541 cggtattcat accttaagcg cccaggcgac cgagaaaatg gagaacgtgc gcaagcgggc
     9601 atcgctgttt attaatgccc gttcggcgga agagctggtg ttcgtccgcg gcacgacgga
     9661 agggatcaat ctggtcgcca atagttgggg caacagcaac gtgcgggcgg gcgataacat
     9721 catcatcagt cagatggagc accacgctaa cattgttccc tggcagatgc tttgcgcacg
     9781 cgttggcgca gagctgcgtg tgatccctct caaccccgac ggtacgctgc aactggagac
     9841 gctgcctacg ctgtttgatg agaaaactcg cctgctggca attactcatg tctccaacgt
     9901 gctgggaaca gaaaatccgc tggcggaaat aatcacgctt gcgcaccagc atggcgcaaa
     9961 agtgctggtg gatggcgctc aggcggtgat gcatcatccg gtggatgttc aggcgctgga
    10021 ttgcgacttt tacgtgttct ccgggcataa actgtatggc cccaccggaa ttggcattct
    10081 ttatgtgaaa gaagccttgt tgcaggagat gccgccgtgg gaagggggcg gttctatgat
    10141 cgccaccgtc agcctgagtg aaggcactac ctggaccaaa gcaccatggc ggtttgaagc
    10201 cggtacaccc aataccgggg gcattattgg tcttggcgcg gcgctggagt atgtttcggc
    10261 gctggggctt aataacatag ccgagtatga acagaatctg atgcattatg cgctatcaca
    10321 gctggaatct gtaccggatc tcactctcta tggcccacaa aacaggcttg gcgttattgc
    10381 ttttaatctc ggtaaacacc acgcctatga tgttggcagt tttctcgata attacggcat
    10441 tgctgtgcgt accggacatc actgcgctat gccattaatg gcctattaca acgtccctgc
    10501 gatgtgtcgg gcgtcgctgg ccatgtataa cacccatgaa gaagtggatc gtctggtgac
    10561 cggcctgcaa cgtattcacc gtttgctggg ataacaggga ggcactatgg ctttattgcc
    10621 ggataaagaa aaattgctgc gtaatttttt acgctgcgcc aactgggaag agaaatatct
    10681 ctacattatt gagctgggcc agcgtctgac tgaattacgc gacgaagacc gaagcccaca
    10741 aaatagcatt cagggctgcc agagtcaggt gtggattgtc atgcgccaga atgcgcacgg
    10801 aattattgaa ttgcagggcg acagcgatgc ggcgattgtg aaagggctta ttgcggtagt
    10861 ctttattctc tacgatcaga tgacgccgca ggatattgtc agtttcgatg tgcgtccgtg
    10921 gtttgaaaaa atggcactca cccaacatct caccccatct cgttcacaag gtctggaagc
    10981 gatgattcgc gcaattcgcg ccaaagccgc tgcacttagc taaactaaag ggacagcttt
    11041 catccggttg gctcacattg ccggatggtc aatgtttaca ctgacctgca caggagtgtt
    11101 ttggtttcag gtgaacataa gcgccatccg acgtttcagc gtgagtctcc ggcaaataca
    11161 ggaggtttac aatgaaacgc gcgtctttgc ttacactcac gcttatcggc gcttttagcg
    11221 ccatccaggc tgcctgggcg gttgattatc cgctaccacc aaccggaagc cgactggttg
    11281 ggcaaaatca aacgtatacg gtgcaagaag gggataaaaa ccttcaggct attgcccgac
    11341 gttttgatac tgcggcaatg ttgatccttg aagccaataa cactatcgtc ccggtgccaa
    11401 aacctggtac gacgataact attccttcac aactgttatt acctgatgca ccgcgtcagg
    11461 ggattatcgt taaccttgca gagctgcgcc tttattatta tccgccggga gaaaatattg
    11521 tgcaggttta tccaataggt attggattgc aggggctgga aacgccggtg atggaaacgc
    11581 gtgttgggca gaaaatccct aacccaacct ggacgcctac ggcaggcatt cgtcagcgtt
    11641 cgctggagcg tggcattaaa ttaccgccag tcgttcctgc cggaccaaat aacccgctag
    11701 gacgttacgc actgcgcctc gcgcatggta atggcgaata cctcattcat ggcaccagtg
    11761 cgccggacag cgttggtttg cgcgtcagtt cagggtgtat tcgcatgaat gcaccggata
    11821 ttaaagcctt gttctccagc gtgcggacgg gaacgccggt gaaagtgatc aacgaaccgg
    11881 tgaaatattc cgtggaacct aacgggatgc gttatgttga agtacatcga ccactatcag
    11941 cagaagaaca gcaaaacgtt cagacaatgc catacacact gccagcaggc tttacgcaat
    12001 ttaaagacaa taaggctgta gatcagaagt tagtcgataa agcgttgtat cgtcgggcag
    12061 ggtatccggt ttcggtgagc agtggagcaa ctcccgcagc cagcaatgcg ccttcagtag
    12121 agtcagcgca gaatggtgaa ccagagcaag ggaatatgtt acgcgcgacg cagtagtggt
    12181 aaatagcaga caaaaaaatg gcgcacaatg tgcgccattt ttcacttcac aggtactatt
    12241 acttgcggta tttagtagcc atgttgtcca gacgctggtt agcacgagct gcgtcatctt
    12301 tagcagcctg aacgtcggaa cgcattgcgt tcacgtcgtt gctcagctgg tcaactttag
    12361 cgttcagagt ctgaacgtca gaagacagct gatcgatttt agcgttgctg gagcaacctg
    12421 ccagcagagt agaacccagg attaccgcgc ccagtaccag tttagtagct ttcattatta
    12481 ataccctcta gattgagtta atctccatgt agcgttacaa gtattacaca aagtttttta
    12541 tgttgagaat atttttttga tgggaaggca cttatttttg atcgttcgct caaagaagca
    12601 tcgaacgctg ggttttagat aaaaaaactg agagaaaaca ggatgcttcc atcggattca
    12661 tcttagataa agtgagatta tatagtgaaa tccgatttga ttttttattg cttctggtta
    12721 tcgattaaat aaaaaagcgc ccatcagggc gcttcgatat acaaattaat tcacaaaagc
    12781 aatattacag gacgtgaaca gatgcggtgt tagtagtgcc gctcggtacc agtgcaccag
    12841 aaaccataac tacgacgtca cctttgtgtg ccagaccgct ctgcagagcc agttctttac
    12901 ccagacggta gaaatcatca gtagaagtga tctctttaac cagctgcggc acaacgcctt
    12961 tgctcagtac caactgatga gccgtttttt cgttggtggt cagtgccagt atggtggcat
    13021 ccgggaagta tttacgtact gcgcgagcag atttaccgcc ctgggtagca accacgatca
    13081 gcggagcatc cagtttttca gcagtttcaa cggcaccacg gcataccgct tcggtaatgc
    13141 gcagtttacg gttgtcattg ttgaactcga gacggctgtt catcacgcgg tcggtacgtt
    13201 cgcagatggt cgccatgata gaaaccgctt ccagcgggta tttacctttt gcggattcac
    13261 cagacagcat cactgcgtca gtaccgtcga ggatggcgtt tgcaacgtca ccggcttctg
    13321 cgcgagtcgg gcgtgggttt ttgatcatgg aatccagcat ctgggtcgca gtgataacga
    13381 ctttacgtgc acggatacat ttttcgatca tcatcttctg ggcgaagata acttcttcta
    13441 ccggaatttc tacacccagg tcgccacgcg caaccatgat gccgtcagag gcttcgagga
    13501 tttcgtcgaa gttgttgagg ccttcctggt tttcgatttt ggagatgatg tggatgtttt
    13561 cgccgccgtg cgctttcagg tgctcacgga tttcgataac gtcagaacgc ttacgaataa
    13621 aggaagcagc aacaaagtct acgccttgtt cgcaaccaaa gatcaggtcc tgtttgtctt
    13681 tttcagccag tgctggcagg gcaatggaaa cgccaggcag gttcacacct ttgttttcgc
    13741 ccaggtcgcc gttgttcagc actttacaga taactttgtt accttcaatg gcagtaactt
    13801 ccataccgat cagaccatcg tcaaccagta cggtgttgcc aacagacagg tcagtagtga
    13861 aaccttcata cgttaccgca accatttcgc tgttgccgat aacagattta tcagtggtga
    13921 aagtaaaggt ctgacccgct ttcagagaaa cgtcgttacc gccttccagt ttcatggtgc
    13981 ggatttccgg acctttggta tcaagcagga tagcggcggt tttaccagtt ttgctcatca
    14041 cgttgcgcag attctgaatg cgctgaccgt gttctgcata gtcaccatga gagaagttca
    14101 gacgcataac gttcatgcca gcgtccagca ttttagctaa catctcttca gattcggttt
    14161 tcggtccgat ggtgcaaaca attttggtct ttttcatgac agtcttagtc tttaagttga
    14221 gaaggatggg agaaacttgc tttctgggcg cactgccgga aagcgaatcc ggtattacga
    14281 aagtggtggt gccttgctct aaggataggt gacatcgaaa aagcgtgcag aggaatgtgt
    14341 gcttgtgtct cagcccaacg tgagtgcctg tttttgagtc gttgagttct acagtccaaa
    14401 gtgctgaaac cattcaagag tcaattggcg cgcattatac gctgaaatta atccaaaagg
    14461 aaaacaaaaa cagcgtttca aaaaatatac aacttgttag acaataaatt gttatcaaag
    14521 atttaacgtt ccagggaaaa ggttacgcaa aaacttcgct gtttgcgcaa cgtctggtgc
    14581 cgaagttgtc agtgtgagga taagtgaaag ttcatccggt gtataaatat atcatcgctc
    14641 aattgctaca gttaaaataa tttatcgcgt tatttgtctg attttgtttg atttttgttt
    14701 aattggttag attgatatat gctgatagtt ggcatgataa ataggtataa agaatgggaa
    14761 atcggacaaa agaggatgaa ctgtaccgag aaatgtgcag agttgtcggt aaagttgtgc
    14821 tggaaatgcg cgatctgggg caggaaccaa agcatattgt tatcgcaggc gtattacgta
    14881 ctgcattagc caataaacgc attcagcgca gtgagctgga aaagcaggcg atggaaaccg
    14941 tcattaacgc actggtaaaa tgaccattca ggatgaggcg gtaagcttca tcctgatatt
    15001 cttgcttcag ttgcggtcca gttaatcatt ctaaataaca actgctgaaa ttatacgaat
    15061 tgcggtatgt attttaatct ataacgataa tattattttt attttctgca atgaacgtca
    15121 caaataattg tcgcttaagt taaatttatt tcaattgata ataaatatca gttcacttat
    15181 aatttcttgc tttgtaattt taattgataa cgatcaatgt taagggctca gaataatcac
    15241 attatttttt ctcctaacca taaaggattg gtttatcggc gttaaatctt ttctcataca
    15301 ctatcgggtt agggaaaata tctggtgttg gggacagagt cagcagcgta ttagttgaat
    15361 gcttaatatc aggctgctga attgtcagca ctgtgtttgc gaagaggatg cgaaataaat
    15421 gaacccggtt gatcgtccac tattagatat tggcttaacg cgattagaat tcttgcgtat
    15481 atcaggaaaa ggccttgctg gtctaacaat tgcccctgcg ttactgtcac ttttgggttg
    15541 caaacaggaa gatattgata gcggcacagt agggttgata aatacaccca aaggggtgct
    15601 ggtgacccag cgagcgcgat gcacgggttg ccaccgctgt gaaatctctt gtaccaactt
    15661 caacgatggc tcagtaggga cattcttctc ccgtatcaaa atccatcgca attatttctt
    15721 tggcgacaac ggggttggct ctggcggcgg cctgtatggc gatctcaact ataccgcgga
    15781 cacctgccgt caatgcaaag agccgcaatg catgaacgtc tgcccgattg gtgcgattac
    15841 ctggcagcag aaagaaggct gtattaccgt cgatcataaa cgctgtattg gctgtagcgc
    15901 ctgtactacg gcatgcccgt ggatgatggc caccgtaaat accgaaagta aaaaatcctc
    15961 gaaatgtgtg ttatgcggtg aatgcgcaaa cgcctgccca acaggggcgt taaaaattat
    16021 cgagtggaaa gatattactg tgtgaatctt gcaaaggaaa aaatcatggc taacggttgg
    16081 acaggtaata tattaagagt caatctcacg acaggaaata ttaccctcga agattccagt
    16141 aagtttaaaa gttttgtcgg tggcatgggc ttcggctaca aaattatgta tgacgaagta
    16201 ccgccaggca cgaaaccttt cgatgaagcg aataaattag tctttgctac cggcccatta
    16261 actggatctg gtgccccctg tagttctcgc gtaaatatca cctcactttc tacttttacc
    16321 aaaggaaatt tagtcgtcga tgcccatatg ggtggctttt ttgcagcgca aatgaaattc
    16381 gctggatacg acgtcattat tatcgaaggg aaggcgaaat caccggtatg gctgaagatt
    16441 aaagatgaca aagttagcct ggaaaaagcc gatttcttat ggggaaaagg gacgcgcgca
    16501 acgacggaag aaatttgtcg attgaccagt ccggaaacct gtgtggcggc tattggtcag
    16561 gctggggaaa accttgttcc tctctctggc atgttgaata gccgtaacca cagcggcggt
    16621 gcgggaactg gcgcaataat gggttcgaaa aacctgaaag cgattgcggt tgaagggacg
    16681 aaaggggtca acattgccga tcgtcaggag atgaagcgtc tcaacgatta catgatgact
    16741 gaacttattg gtgcgaataa caaccatgtc gtgccaagta cgccgcaatc gtgggcagag
    16801 tattcagatc ccaagtcacg ttggacagca cgtaaagggc tgttttgggg cgcggctgaa
    16861 ggtggtccga ttgaaacggg ggaaattccg ccaggcaatc agaacacggt cggctttcgt
    16921 acctataaat ccgtttttga cttaggtccg gcggcagaga aatacacagt aaaaatgagt
    16981 ggctgccact cttgcccgat ccgttgtatg acccaaatga atattcctcg ggtgaaagag
    17041 tttggcgtgc ccagcacagg tggtaacact tgtgtagcca actttgtcca taccaccatc
    17101 ttcccgaacg gtccgaaaga ttttgaagat aaagacgatg gtcgtgtgat tggtaacctg
    17161 gtgggtctga atctgttcga tgactacggc ctatggtgta actacgggca gttgcatcgc
    17221 gactttacct attgttacag caaaggtgtg ttcaagcgtg ttctgcctgc tgaagagtat
    17281 gcggaaattc gctgggatca actggaagcg ggtgacgtta acttcattaa aattaaagat
    17341 ttttactacc gtctggcgca tcgtgtgggt gagctgagtc acctggctga tggttcatat
    17401 gccatcgcag aacgctggaa tttgggtgaa gagtactggg gctacgcga
//
LOCUS       DAJVQG010000084        17421 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJVQG010000084 DAJVQG010000000
VERSION     DAJVQG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.084"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(76..807)
                     /gene="rlmB"
                     /locus_tag="OQM83_002498"
     CDS             complement(76..807)
                     /gene="rlmB"
                     /locus_tag="OQM83_002498"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCS1520739.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(987..3428)
                     /gene="rnr"
                     /locus_tag="OQM83_002499"
     CDS             complement(987..3428)
                     /gene="rnr"
                     /locus_tag="OQM83_002499"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCS1520740.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYVPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="OQM83_002500"
     CDS             complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="OQM83_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCS1520741.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4097..5395)
                     /gene="purA"
                     /locus_tag="OQM83_002501"
     CDS             complement(4097..5395)
                     /gene="purA"
                     /locus_tag="OQM83_002501"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCS1520742.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5499..5696)
                     /locus_tag="OQM83_002502"
     CDS             complement(5499..5696)
                     /locus_tag="OQM83_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCS1520743.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="OQM83_002503"
     CDS             complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="OQM83_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCS1520744.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="OQM83_002504"
     CDS             complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="OQM83_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCS1520745.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="OQM83_002505"
     CDS             complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="OQM83_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCS1520746.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="OQM83_002506"
     CDS             complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="OQM83_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCS1520747.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="OQM83_002507"
     CDS             complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="OQM83_002507"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCS1520748.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="OQM83_002508"
     CDS             complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="OQM83_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCS1520749.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="OQM83_002509"
     CDS             complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="OQM83_002509"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCS1520750.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="OQM83_002510"
     CDS             complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="OQM83_002510"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCS1520751.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="OQM83_002511"
     CDS             complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="OQM83_002511"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCS1520752.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16016..17155
                     /gene="queG"
                     /locus_tag="OQM83_002512"
     CDS             16016..17155
                     /gene="queG"
                     /locus_tag="OQM83_002512"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCS1520753.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17138..17191)
                     /locus_tag="OQM83_002513"
     CDS             complement(17138..17191)
                     /locus_tag="OQM83_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1520754.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3951 a         4975 c         4369 g         4126 t
ORIGIN      
        1 tcaccgtaat tatcttacca gctatagaca aaaaaaacca tccaaatctg gatggctttt
       61 cataattcag acgaactagc tgcgctgacg aaccgcttca aataagcaaa ttccggttgc
      121 aaccgaaacg ttcagggaag aaacacttcc tgccattggg atgctgatca actcatcgca
      181 atgttcacgg gtcaggcgac gcataccttc accttccgcg cccatcacca gcgccaagcg
      241 tccggtcatt ttgctctgat agagtgtatg atccgcctcg cctgccgtac cgacgatcca
      301 gatattctct tcctgcaaca tacgcatggt gcgcgcaagg ttagtcaccc gaatcagtgg
      361 aacgctttct gccgcgccgc aggctacttt tttcgccgta gcgttgagct gtgcggagcg
      421 atctttcggc acaatcaccg cgtgaacgcc agcagcgtcc gcgctacgca ggcacgcgcc
      481 gaggttgtgc ggatcggtta caccgtcgag gatcagcagg aacggttgat cgagcgaagc
      541 gatcagatcc ggcagatcgt tttcctgata ctgacgtcct ggcttcacgc gggcgataat
      601 gccctgatgc acggcaccgt cgcttttctc gtcgagatat tggcggtttg ccaactggat
      661 aaccacgccc tgagactcaa gggcgtgaat cagcggtaac agacgtttat cttcacggcc
      721 ttttaaaata aagacttcct gaaaacgttc aggggcgcgc tccagcaggg cctgcactgc
      781 gtggatgccg taaatcattt cgctcattaa tgtactcgtt gtttacgttt tgcctgatgc
      841 actacgttta tccggcctac atgatctctg caatatattg aatttgcgtg attatgtagg
      901 ccggataagg cgttcacgcc gcatccggca tgaacaaagc gcattttgtc agcaatctaa
      961 ccctcttctt ttaaagaggg tattgatcac tctgccactt ttttcttcgc cgcacgcttc
     1021 gctttggtcg ctgcggctat tttctgcgtt ttcgccgatg gctttttcgc ttttctcgcg
     1081 tctttcttcg ccgctttcgg cttcgttttt ttctcaccgc ggaaggcgct gtctggctca
     1141 aagtttacct ttttaccgac ctgacgacgc ttgccgcctt ttttgcctgc atcgcctttt
     1201 ttcgctttct cgcgcgccgt tttaccgacg ttgcgcggtg cgcgttcact ggagatcaga
     1261 ctaaagtcga ttttgcgctc gtccatatta accgcttcga cgcgaacttc cacgcgatcg
     1321 cccaggcgat aagtctggcc gctggattcc cccatcaggc gttgccctac ctggtcaaag
     1381 cgatagtagt cattgtccag cgaagagaca tggaccagac catcaatgaa caagtcgtcc
     1441 agacggacga agaagccaaa gccagtgacg ctggaaatta cgcctttaaa gacgttacct
     1501 acctggtcga gcatgaagtc acacttcagc cagtcagcga catcgcgcgt tgcttcgtcg
     1561 gcacgacgtt ccgccatcga acagtgctga cccagttgca acatctcttc catcgaataa
     1621 tggtagccgc cggtttcagt ggtgttgccc tgatgcccct gctctttcgc cagcagatac
     1681 ttaatggcgc ggtgcagcgt caggtcagga taacgacgaa tcggcgaagt aaagtgcgca
     1741 taggactgca atgccaggcc aaagtgacca cggttttctg gatcgtaaat cgcctgtttc
     1801 atcgagcgca gcagcatggt ttgcagcatt tctgcatcag gacggtcggc aaccgactcc
     1861 agcagttccg cgtagtcacg cggttccggc ttgttaccac ccggcagctc cagccccagc
     1921 tccgccagca ctgaacggaa agaggtaatc gcttcggtgc tcggcttgtc gtgaatacgg
     1981 aacagtgccg gttctttcgc tttctcaacg aaacgcgccg ccgagatatt cgccagaatc
     2041 atgcactctt caattaattt gtgcgcgtcg ttacgctggg tctgttcgat acgttcaata
     2101 cggcgttcag cgttgaaaat gaacttcgct tcttcgctct caaatgagat cccaccgcgt
     2161 tcttcacggg ctttatccag cactttatag aggttatgca actcttcgag atgcttaacc
     2221 agcgggacgt actgctcgcg cagatcctga tcgccctgca gaatatgcca gactttggtg
     2281 taggtcagac gcgcgtgaga gctcatcacc gcttcgtaga atttgtagcc cgtcaggcgg
     2341 cctttcgacg aaaccgtcat ctcgcacacc atacacaggc ggtctacctg cgggttgagc
     2401 gaacacaggc cgttagagag cacttccggc agcatcggga taacctgcga agggaagtac
     2461 accgacgtgc cacggttacg cgcttctctg tccagcggcg ttggcggacg cacatagtag
     2521 ctgacgtcgg caatcgcgac ccataaacgc cagccgccgc cgcgtttttt ctcgcagtaa
     2581 actgcatcgt caaagtcacg ggcgtcttcg ccatcaatgg tgaccagcgg taaatcgcgc
     2641 aaatcgacac ggcccgcttt tgcttcttcc ggcacttctt ctttcagccc cgcaacctgt
     2701 tgctcaacag cctgcggcca gatatacgga atttcatggg tacgcagagc gatatcaacc
     2761 gccatgccgg tgcccatatt gtcgcccagc acttcgacga ttttacccac cgctttggtg
     2821 cggcgagtcg gacgctgagt cagttcgact acgaccacaa agcccatccg cgcgcccatg
     2881 atctgatcgg gcgggattaa gatatcgaag ctcagacggc tatcgtcagg aaccacaaag
     2941 ccgacgcccg cttcggtaaa gtagcgacca acaatctggc tggtttttgg caccagtacg
     3001 cggacaatac gcgcttcacg acgaccttta cggtcagcac ccagcggctg cgccagcacc
     3061 tgatcgccat gaatacaggt tttcatctgc tcgctggaga gatacaaatc atctttacgc
     3121 ccttcaaccc gcagaaagcc gtagccatca cggtggccaa taacggtacc tttcaccagg
     3181 tcgaggcgtt ccggcagcgc atagcactga cgacgagtga agaccagttg accatcgcgc
     3241 tccatcgcgc gcaggcggcg acgcaggcct tcaagctgct cttcgccttc aatgtgcagt
     3301 tctaccgcca gctcatcacg gctggccggt ttttcacgtt tggttaaatg ttcgaggata
     3361 aattcccgac tagggatggg attcgcgtat ttttcagctt cgcgttcctg gaaaggatct
     3421 tgtgacatct cggttcctcc gttgtcatct ctgatgaaga ttttcgtcac tccaccagca
     3481 ataatttata aagcggttga ttctcttcaa ccaaatcggc aagcgtgtag ttatccagtt
     3541 ccgtaagaaa actttgcacg gccttagaaa gcgcctgttt caaccgacag gcaggtgtaa
     3601 tgtggcaaaa ctcactgctg caattcacca gcgataaggg ctccagctcg cgcaccacat
     3661 caccaatacg tatcgcactc gccggtttac ccaggcgaat gccgccattt tttccacgaa
     3721 cagcagtcac gtagccggca cgactaagtt gattgattat tttgaccata tgattacggg
     3781 agacgccgta gacgtcagtc acttcagaaa tactggtcat ccgcccttct ggcaatgacg
     3841 ccatgtagat cagcgcacgt aatccgtaat cagtgaaact cgttaactgc acatcaacct
     3901 caaaagggaa atcgggaaaa aatacattta tattgatgat aaaccagcca caagctgtgt
     3961 cgctaattta tttcagttcg gggaaggaaa aaagcgagga tttaacactg tgtcggatag
     4021 cgtaatggct taaccgacac agcaatgtat cacactgtta gcccggcagg caaaatatct
     4081 gccaggcgta ccagaattac gcgtcgaacg gatcgcgcag aatcatggtt tcagtacgat
     4141 ccggaccggt agagatgata tcgatcggca caccggtcag ctcttcaata cgcttgatgt
     4201 agttcagcgc cgcctgcggc aggccgctac gatctttcac gccgaaagtg gattcagacc
     4261 agcccggcat ggtttcgtaa atcggctcta cacctttcca gtcgtcagct gccagcggag
     4321 tggtagccac ttcgcgacca tccggcatac ggtaagccac gcagagttta acctctttca
     4381 ggccatccag aacgtccagc ttggtcaggc aaaagccaga cagggagttc agctgtaccg
     4441 cacgacgaac ggcaacggtg tccagccagc cggtacgacg acgacgaccc gtagttgcgc
     4501 cgaattcgtt accctgcttg cagaggaact cgccagtttc atcaaacagt tcggtcggga
     4561 acggacctgc acctacacga gttgagtaag ctttgaggat acccagaaca taatcaacat
     4621 aacgcgggcc caggccggaa ccggtcgcca cgccaccagc agtggtgttg gaagaagtta
     4681 cgtacggata agtaccgtgg tcgatatcca gcagcgtacc ctgcgcacct tcaaacatga
     4741 cgaaatcgcc acgctgacgc gcctggtcga gcaggtcaga aacgtcaacc accatagaag
     4801 tcaggatgtc ggcaacagcc atcgtatcat ccagaacttt ctggtaatca accgcttcag
     4861 ctttgtagta gttaaccaac tggaagttgt gatattccat cacttctttc agtttttcag
     4921 cgaaggtttc tttgtcgaaa aggtcgccaa cacgcagacc gcgacgtgct actttatctt
     4981 cataagcagg cccgatacca cgaccggtgg tgccgatcgc tttcgcgcca cgcgctttct
     5041 cacgcgcgtt atccagcgca acgtgataat caaggatcag cggacatgct tcagacagca
     5101 gcagacgctc acgaacgggg atgccacggt cttccagttc tttcatctct ttcatcagcg
     5161 cggccggaga cagcacaaca ccgttaccga tgatgctggt tacattctcg cggagaatac
     5221 ctgatggaat aagatggaga acggtttttt caccgttgat tacgagagta tggcctgcgt
     5281 tgtgaccgcc ctggtagcgt acaacatatt tagcccgttc agtcagaaga tcgacgatct
     5341 tacctttacc ttcgtcaccc cattgggtgc ccagtacgac gacgttgtta cccatttttc
     5401 aaaatcaccg tttgcttaaa aatggattct accatcgctt tttcagagtt acagcacttt
     5461 ttgcactcaa aaatgacctc gatcagtctg ttttttgttc agccaatcgt tttcctcaac
     5521 atgtagtaga ccacaacgcc cgcaaccaca agtccaccgc caaaacgacg taaaatatta
     5581 tcgggcaaat tggtcatcgc agagatcatc ttcttccatg ccttcgggta aagcatcggc
     5641 cctaaacctt ccagtaccaa aaccagggca agcgccagcc agattgtcga attcattcat
     5701 tatccttata gaaaaagaaa accaccgaca tccctgagga tgcggtggct ttattgacct
     5761 gtaccgcagt cgttatatta acgcgttgcg gaagtcggcg tcttcatgta gcggaagaaa
     5821 tcgctatccg ggctcatgac catcacgtcc tgattgccag agaagctgtt ctcataagca
     5881 cgcaggctac ggatgaatgc gtagaagtcc ggatctttac tgaatgcatc agcaaacagt
     5941 ttggctgctt cggcatcgcc ttcaccacgc atgatgcggc cctgacgctc agcttctgcc
     6001 agcgttctgg tcacttcata gtcggcagtc gcgcgcagtt tttccgcttc ttcctgacct
     6061 tgtgaacggt gacgacgcgc taccgcttca cgctcggcgc gcatacggtt gtagatcgct
     6121 tcagacactt cggtcggcag gttgatctgc ttgatacgca catcgacaac ttcaataccc
     6181 agcgccgcca tactgttcgg gttgatgacc ggaactttgc ccttcgtctc agccgttacg
     6241 cgctctgccg cttcggcaat ggcgttatct gccgccgggg tagtaacttc atcttctgta
     6301 cccgcagaac cggagttcag cgcgtcacgt acttcgaggg tcagacgacc acgggaatcg
     6361 gtgacgatgt ctttcacgtc caggcgacca atttcagaac gcagacggtc agagaactta
     6421 cgtttcaaca gcacttccgc ttgcgaaatg tcgccaccac ccgttgccag gtagtaacgg
     6481 ctgaaatcgc tgatgcgcca tttgatgtaa gaatcgacga tcaggtcttt cttctctttg
     6541 gtcacaaagc ggtcggcctg gttgtccatg gtctgaatac gtgcgtcgag cattttcacc
     6601 gtttcaatga acggtatctt gaaatgcaga cccggctcat aaaccagagg tttgttgtca
     6661 tcgtcacgca gtaccttacc aaaacgcagc gtaataccgc gctcaccttc tttgacgaca
     6721 aagacagaca tgtaaagcac taccagcacg atgatgataa tcgcgataac tgacttacgc
     6781 atcgttattc cccctgacgc tggtagtcgt tacgctgcgc gttggcgcgg cgttggtcca
     6841 taatatcgcc ctgactggtg gacgacgtgt tgcttgctcc actggttgtg gaggaagagg
     6901 ctggcggcag acgcagcaga ttgctggcac cgttatcgct cttcgccgca ggggcgttac
     6961 cgcctttcag catctggtct aacggcagaa ccatcaggtt gccaccttta tcgttaacca
     7021 gcactttgcg ggtgttaccc aacacttttt ccatcgtctc gatatacaga cgctcgcgag
     7081 taatttccgg cgcggcttta tattccggca gaagtttagc aaagcgcgcc acttcaccct
     7141 gagcttccag gatggtctgg gccttgtacg cacgcgcctc ttcgaggata cgttgcgcct
     7201 gaccgttcgc acgcggctga acttcgttgg tatacgcttc tgcttcacga atgtattgct
     7261 gttcgttttc acgcgcggca atcgcatcgt caaacgccgc ttttacttct tccggcggac
     7321 gagctgcctg gaagttgacg tccagcagag tgatacccat gtcatacgga cggatagtct
     7381 cttccagttc gcgctgagta tcgctacgaa tcacggtacg accttccgtc agaatgcggt
     7441 ccatggtgta tttaccgata actccacgca gggcgctgtc ggtagcctga cgcaggctgt
     7501 catccgggct ggtcacgcta tacagatatt tttccggatt ggtgacgcgg tactgcacgt
     7561 tcatctcaac gcgcactacg ttctcgtccg acgtcagcat cacaccagaa gcggccagtt
     7621 cacgcacggc ttccacgttc accggtttga cttcgtcgat aaacgtcggt ttccagttca
     7681 gacccggctc aaccagatgg ctgaatttac caaagcgtgt taccacgccg cgttcggctt
     7741 ctttaatggt atagaaacca ctggccgccc agataatgac aatcgctgcc gctgcgatgg
     7801 taacgacacg accgccaagc tgcgggcgcg ggccttgcga tgaactgcca ccgccagatc
     7861 cggtgccttt accgccgccc agaccaccga gctttttgct cagtttgcgg aagatatcat
     7921 ctaaatcagg tggcccttga tcgcgaccgc ctttgtttcc atttccctca gagttgccgc
     7981 caggtttgct gcttccccac gggtcgcggt cttgtccgtt attaccgggc tgattccacg
     8041 ccatgtttgt gctccatatt tgttatgcgg tgatccctgt tgtctttcga cgccagagga
     8101 tatgactcca cgcttcagac gctacgccgt tagatcaggt aatcgatcaa cgccggttct
     8161 tgtttacaga ggcgacgcca gtcaacgatc ggcatacgaa cttgcagact tacgctgccg
     8221 tcctcctcca tccactcttt ttctattgcc tgaagctgat aaaaacgact tctcagacgt
     8281 ccttcctgcg gcggcaaacg caatgtatgc tgcgccacct cgccggaaag ccgctccgtc
     8341 aaagcctgaa aaagctgtgg tatccccgct ccggtctgtg cggaaagcca gacacggatc
     8401 ggtttgttct cttcgtcccg atcaatacgc ggttcgaaat cttccagcat atcgatcttg
     8461 ttcatcacca gcagggttgg gatctcgtga gcgtcgatct cttcaagaac cgtattcacc
     8521 gcttcgatgt tttcttgtac acgtacatcc gccgcatcaa tgacgtgcag cagtaatgtg
     8581 gcttgccgcg tctcttgtaa cgtggcttta aatgctgcca ccagatcgtg cggcaggtgg
     8641 cgaataaacc ctacggtatc tgcaagtacg gtttcaccga catccgcgac gtcaatacgc
     8701 cgcaacgtcg ggtcgagggt ggcaaacaac tggtctgccg cgtagacccg cgcttcggtg
     8761 atgcgattga aaagggtaga tttaccggcg ttggtatatc ccaccagcga aacagtagga
     8821 acgtcggctt tgatacgcga ttgccgcccc tgctcacgct gcttttcaac tctttctagg
     8881 cgcgactgta tctgcacgat gcgattacgc aacaaacgac ggtcggtttc gagctgggtt
     8941 tcacccggac cacgcaaacc tatcccgcct ttctgtcttt caaggtgggt ccagccacgc
     9001 accaggcgcg tagccagatg gcgcagctgc gctagctcaa cctgcaactt accctcatgg
     9061 gtacgcgcac gttgggcgaa aatatctaaa ataaggccgg tgcggtcgat aacacgacac
     9121 tcgcacaaac gctccaggtt acgctcttgc gccgggctca gggcatggtc aaaaagaacg
     9181 accgaagcac ccgtcgcttt gacagcttcc gcaatttcaa ctgctttacc ttcacctaca
     9241 aaatactttg ggtgcggcgc tttacggcta ccggtaatca cctgcaatgc ttcgacaccg
     9301 gcggaagaga ccagagattc aaactcctgg aggtcttcca tatctttgtc ttgcgtaaaa
     9361 tagatgtgta ccagtaccgc ctgctcacca gcatcataac ggtcaaacaa gcgtataacc
     9421 ctctaaatag atcagcgggg aacgcaggat cgctggctcc ccgtgtaaaa aaaacagccc
     9481 gaaaccttat tcggtttctt cgctgtcctg ttgcgcggaa gtattctgcg cgctgctacc
     9541 atgatggtag ttactgctgg taccgccacc ggcgttgtta ctgtgatgag aaaccgggcg
     9601 agacgggaca acagtagaaa tcgcgtgctt gtaaaccatc tggctgaccg tgtttttcaa
     9661 caggatcacg aactgatcaa aagactcgat ttgcccttgc agcttaatac cattcaccaa
     9721 ataaatagaa actggaacac gttcccgacg cagtgcgttc aggaacggat cttgtaaaga
     9781 ttgcccctta gccattctct cttttcctta tatgcttatt tgtactttga acctttcgat
     9841 tctgaaaaaa ttgcgcacga tacgtctcaa ttgtacacat tcagcctgcg atagcaccaa
     9901 caacctgtaa tacttcgtca cgcgcctgtt ctggtttttc actgtcaagc cagtgaaccc
     9961 cttcccaacc acgcagccag gttatctgcc gcttcgccaa ctgtctcgtg gcgcaaacac
    10021 ctcgataaac catttcatcg tatgagattt cgccttcaag gtaagaccac atctggcgat
    10081 aacccacgca acgaatggaa ggcaagtccg tatgcaaatc tcctcgggca aaaagcgccc
    10141 ggacttctgc ttcaaaacct gaagccaaca tctgatgaaa acgctgctca atgcgttgat
    10201 ggagcagttc acggctcgcc ggggcgatgg cgaactgatg cacctgatac ggtagagcgt
    10261 ctcctgacgt ttgcgtcagt tccgttaaag ttttacccga aatgaaaaaa acttccagtg
    10321 cccgggaaag cctttgtgga tcatttggat gaatccttgc tgccgcaacc ggatctacct
    10381 cctgaagttg acgatgcaat gactcccaac cttgctctgc cgcctgttgc tcaattctgg
    10441 cccgcacttc cgggtctgcc gacggtagcg gcgacaaccc ttccagcaat gccttgaaat
    10501 acaacatcgt accgcccact aacagtggga tccgccccgc cgcggtgata tcggccattt
    10561 ccgccagcgc atcgcggcga aaatcagcag ccgagtaagc ctgcgacgga tcgcgaatat
    10621 ccagcaatcg gtgcggcgcg gcgagtaact cttcagcgtt cggcttcgcc gtcccgatat
    10681 ccatcccttt gtaaataagg gcagaatcaa cgcttatcaa ctctactggt aaaattttac
    10741 gcagctcaat ggctaacgcc gttttaccgg aggccgtcgg ccccatcaaa aaaatcgcct
    10801 taggcaggct cgccttactg atatcactca tctttcaggg cttttatcgc cggatgtaaa
    10861 tcaacagatt gtaacagacc acccggcggc gttttcacaa gttgcggaca taaccgttcc
    10921 acgtccgcca gcagggttat ggcctgtgcc attgaccact gcgcatgttc gctcatcaga
    10981 tttcgtgcaa tccactgcgc aatattgcca ggttcgaata cggactgctt cgccaggtag
    11041 cctatcagtt caggaatcaa gatttgtaaa ttttgttggc gtaagggtaa aggcacggcc
    11101 ctgatggtca catgctgtgc atctgactgg aaatcaatac ccaattccgc cagggcagac
    11161 tgcgcttttt ctaatgccga tttttcttcg ccagaaactt ttagccgcaa cggaataagc
    11221 agcggctgag cgcaaactgg cgcttcaccc ggcgtcagtt gtacctgacg cagccaacgt
    11281 tctgccactg gcaaggctaa aagtgaaatg ttgccgtcgc gctccagcaa cgcacagtcg
    11341 gaatggacga tagtcagtac ccgaccaaaa ctctgactgt tcgccgcaag tgcaggttcc
    11401 tgcggttccg gcgcttttaa tttttgcatc ggcgcgggcg tttgcaaaag ctggcgatac
    11461 acttcacctt gctgtttctg gtagcctggc tgcgcattcg gccagggggc agccggacga
    11521 ctgcctgatg ctggcgcagg agtgtagcgc ggagctaccg gctcacgagc tgccggttct
    11581 gcaaagtgat tgcgccccgc cgccacgcgg ttttccggaa tggaacgcgg tgcaggttgg
    11641 ggttcatcgt ccagcggtag cggcgtttcc agttgctgtt gtagcacgct cagcacgccc
    11701 tgatagataa aatcatgcac cagacgcgac tgatggaaac gcacttcgtg tttggcgggg
    11761 tgcacgttga cgtccacctg atgcggatcg atctccagat acagcacaaa tgccggttgc
    11821 tgatcggccc ccagtttgtc ttcgcaggcc tggcggatcg cgtgattgat caggcgatcg
    11881 cgcatcatgc gaccgttcac gtagcaatac tgaatttctg ccagtgcggg cgtggtgtga
    11941 tttggatcgg ccacccagcc gcgtagcgtg agatcgccgt gttgccattc aatcgccagc
    12001 gcttgttcaa gaaaagcggt gccgcaaatc gcgcctaagc gccgttcttt ttgcccgcct
    12061 tccggcactg cgcggtactg acgcacaatt ttaccgttat gcgacaggtt gatcgtgacg
    12121 tcgaaacgcg ccagcgcaat gcggcggatg atctcatcaa tgtggttaaa ttcggttttc
    12181 tcggtgcgca ggaatttgcg ccgcgccggg gtgttgtaga acagatccag cacctccagc
    12241 gtcgtcccca caggatgcgc cgccggtttt accgtcacgt tcatatcgcg cccttcggca
    12301 taggcctgcc aggcttcctg ctgttctgcg gtgcgtgaag tgagcgtcag gcgggaaacc
    12361 gaactgatac tcgccagcgc ctcaccgcga aagcccaggc tgataatggc ttcgagatcg
    12421 tccagagagg cgattttact ggtggcatga cgagccagcg ccagcgccag ctcatctttt
    12481 ttgataccgc agccgttatc acgaatgcgg ataagtttcg ccccaccgcg ttcgatatca
    12541 atatcgatac gcgtcgcacc tgcatcgagg ctgttttcca ccagttcttt gactaccgac
    12601 gcaggtcgct cgaccacctc acctgcggca atctggttcg ccagttgtgg cggtaagacc
    12661 tgaattggca tcaatcgtcc ttagtttggc agcgtgcgat ctggcgtcgt caccgtactg
    12721 gcagtttgtg ccgttgcccc ctgcggcgca gattgcatcg gatgcgcaag gaaataattg
    12781 cgcaggcctt tgtaaatggc ttctgccagc tgttgttggt aatcgtcgct cgccagcaaa
    12841 cgttcttcgc tgttgttgct gataaaaccg gtttcgacca gtactgacgg gatatccggc
    12901 gaacgcagaa cgccgagact cgcgtgttct ggtcggcgtt tatgtatttc gccaatgcgt
    12961 tgcaactgac tgatcatact ggtcgctaca tcatacccta cccgctggga atgaccgaac
    13021 tgtaaatcca gcaccgcctg gcttaaatag gggtcagact gactgttcgc cagcacatcg
    13081 cccgccccac ccagcagctc cgactgtttc tcgtgctgct ccagccagct ggccatttcg
    13141 ctgttggcgc gacggttaga gagcacccat acggaagcgc cagtcgcact gcggttcggt
    13201 gcggcatcag cgtgaatcga cacgaggaaa ttggcgtttt gcttacgtgc cacatcgctg
    13261 cgccccatca ccgagataaa gtaatccccg tcacgggtta aaacgccttt aaacatcgga
    13321 tcgtcattga gcaaagtgcg caatttacgc gcgatggcga tggtgacatt tttctcccgc
    13381 gtaccaccgg ggccgatagc gccagggtcc tgaccgccgt gtccggcatc aatagcgatg
    13441 ataattttat cgccagtgtt agccgtcgcg cgcgctgccg gacgcgttac cgtattactg
    13501 ctgataacac ccgtagtgcg gttactttcc gttttaaacg gattgcgcgc cggttcgctg
    13561 acgcgcggtg cgacaaccgc aggcgtttca acgcgtttcg caaccacggg cggtggagga
    13621 ggcggtggcg gcacatcggc gttaatcgta aagacgacag tgtaattgct gccattctgc
    13681 cgcttcaccg cttcggtttt accgttttcg gtaagatcga ccaccagccg tagcgtttgt
    13741 gcatctttag gcgttccaga gcgaatcgcc ttcaccagat tattgccgct gaacaacaac
    13801 ggcagtccct gaatcacgcc cgtttgtttg atatcgagcg ccacggtgcg tttgctttga
    13861 tggctaaacg cataatcagg atcgccaata aaactcaacg ttatccgcgc ctgttgatta
    13921 ccgttagaaa cctgaatatc agagagcgtc gcggcaccca ccggcgtgca cagcagcagc
    13981 agcgtcgcta ccaaccaatt tctgatgcga tacatcatcc cgccaccttt caaaggttaa
    14041 ccggctaaac gcgccagcaa caattcaccc gcagaggaaa ccgcactcac gcgcgcctca
    14101 cggccttgtg cctgataatc aatgtgtatt tcgacatccg ggtcaggaag aacacctgta
    14161 ccttgttgtg gccactccac caggcagatg gcatcgttgg caaaataatc gcggatcccc
    14221 ataaactcca gctcctcggg atcggcaagg cggtacaaat caaagtgata aaccattaaa
    14281 ttgtcgagcg tatagggttc gaccagcgta taagtggggc ttttgacatt accctgatga
    14341 cccagagctt gtaaaaaacc ccggctaaag gtggttttac ctgcgcctaa atcgccatac
    14401 agatagatta cggttgcgcc atcgcaggct ttcgctaccc gctcgcccag gtctaatgtt
    14461 gcctgctcat cagggagcgg aattactcga ttcatcatgg tttttatcag tcacttccgg
    14521 gttaacaata cgctgtagcg tggaaaagag atcggttgcc agcatcccgc gcgttccaaa
    14581 acgcgccgcc agtacgtcag ctgccgcacc gtgcgcgaca cagcccgcac aggcggcatc
    14641 atacggcgac agtttttgcc caagcaatgc gccaataata ccagagagca catcgcccat
    14701 gccgccgctc gccatgcctg catttccggc atcaataatg cctaaagcgt caggatgggc
    14761 ggcgaccacg gttccggcac ctttcagcac cgctacgccg ccataacgtt gtaccagacg
    14821 tttggcgcaa tgtaagcggt cactttcaat ttcagcgacg gaacagccta acaaccgtgc
    14881 ggcctcgcca ggatgcggcg tgatcacgcg attgtgacgc ttatcgggat tgattgccag
    14941 caggttcaat gcatcggcat cccacaacat cggtttgcga aaattctcaa ctttttgcag
    15001 tgcttttttc ccccactctt gctggcccag accgggacca atcaccacca catcggccca
    15061 ttccaggctt tcggtaagag agtccatcgt cagttcatgc accatcaatt ccggtcgtgc
    15121 agtcagcagc ggcgcaatgt tttcactgcg ggtcagtact cggactaaac cagcaccagc
    15181 acgcagcgcc gcttcccccg tcatacgaat agcccccgcc gtgccgtgat cgccaccgat
    15241 aatcaccagc cgcccgtgat cgcctttatg cgaagtcggg cgacgcggtt tcagccagtg
    15301 agaaagttgt tctgccgaaa accgctgaat tttcgtctcc tgacctgcca gccagctatc
    15361 cagccccagt gagtcaaaat gcagttgtcc tgtaacatcc cgcgcttttc cagtaagcaa
    15421 gcctggtttc agcgcaataa aagtgatggt gtgatcggcg ttgatcactg cgcctggcgt
    15481 agcgccggtt tcagccagca ggccggaagg gatatcaacc gccacaatcg gcgcaggatg
    15541 ggaattagcg tggtcgatta actggctaat ggattcgcgg ggcgcttgct gcaagccggt
    15601 accgagcagc gcatcaacaa tcagatctac cgattcgggc cagacaatat tcgaagcatg
    15661 gatctcgcca cccgcgttta accatgcttc gcgtgccagc gcggcctctt ccggcaacgg
    15721 tttgtcgctc tcctgggcca acaacgtgac ctcaatgccg accgctttgg ccagtcgcgc
    15781 gaccacgtag ccatcgccgc cgttattacc atgaccgcac agcaccagcc agtggcgggc
    15841 gtcaggatac gccgaacgac acacctggaa tgcggcctcg ccagcgcgaa gcatcagctc
    15901 atagagtgtg agccccagca catctgccgc ctcgcgttct ccgcggcgga tatcgtcggc
    15961 gtaccagacg gtgtgtggta tacttacggg gtttttcttc attgtatggt ccgtcatgtc
    16021 agagcccctc gatctcaatc agttagcgca aaaaattaaa cagtgggggc tggaactggg
    16081 ctttcagcag gtaggtatta ccgataccga tctcagcgag tccgagccca aactgcaagc
    16141 atggctggac aaacaatacc acggcgaaat ggactggatg gcacgtcacg gtatgctgcg
    16201 cgctcgcccc catgagttat taccaggtac gctgcgtgta atcagcgtgc ggatgaatta
    16261 ccttcctgct aacgccgcat ttgccagcac gctgaaaaac cccaaactcg gctatgttag
    16321 ccgttatgcg ctgggccgtg actatcacaa acttctgcgc aaccgactca aaaagctggg
    16381 cgagatgatt cagcaacatt gtgtttcgct gaattttaga ccgtttgtcg attctgcgcc
    16441 tattctcgag cgcccgttag ctgaaaaagc tgggctcggc tggacaggta agcactcact
    16501 tatcctcaat cgcgaggccg gttctttctt ctttttaggc gaattgctgg tcgatattcc
    16561 gctgcccgtg gatcaaccag tcgaggaagg atgcggtaaa tgcatagcct gtatgacgat
    16621 ttgcccgacc ggtgccatcg tcgagccata taccgtcgat gctcgccgct gtatctctta
    16681 tctcaccatc gaacttgaag gggcgatccc ggaagagttg cgaccgttaa tgggaaaccg
    16741 tatttacggt tgcgatgact gccagcttat ctgcccgtgg aatcgctatt cgcaactcac
    16801 tacagaagac gatttcagcc cgcgtaagcc gctacacgca caggaactca ttgagttatt
    16861 tgcctggagc gaagagaagt ttttaaaagt cacggaagga tcggcgattc gccgtatagg
    16921 tcacctgcgc tggctgcgta atatcgccgt ggcattaggc aatgcgccct gggatgaaac
    16981 gattctggcg gcgctggaga gtcgcaaagg tgagcaccca cttcttgatg agcacatagc
    17041 gtgggcgatt gcgcagcaaa tcgagagacg aaatgcgtgc atagtcgaag tgcaattgcc
    17101 gaaaaaacag cgtctggttc gggtgattga aaaagggtta ccgcgtgacg cctgattcat
    17161 ccacagcttg tgaataaaaa taaaaacgca ttgcaattca agacgcataa aatcgtcaag
    17221 tgatcgactc gacaaaacgg aatcttgatt tttaacttat ttatcaatag gttaaaaaaa
    17281 taccattcac cgtgcgaagt ttctttgaaa ttaagattac agcctgagga tgtggataac
    17341 tctgtttaca agagtttgtc agagacaaca aaaaccagca ataacgcatc gcgtcgctgt
    17401 ggatatttta ttgagagaag a
//
LOCUS       DAJVQG010000085        17395 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJVQG010000085 DAJVQG010000000
VERSION     DAJVQG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.085"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..128
                     /locus_tag="OQM83_002514"
     CDS             <1..128
                     /locus_tag="OQM83_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HCS1520755.1"
                     /translation="VQVHISAFTPRDAEVLIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            575..880
                     /locus_tag="OQM83_002515"
     CDS             575..880
                     /locus_tag="OQM83_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1520756.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            987..1631
                     /gene="gfcB"
                     /locus_tag="OQM83_002516"
     CDS             987..1631
                     /gene="gfcB"
                     /locus_tag="OQM83_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HCS1520757.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1628..2374
                     /locus_tag="OQM83_002517"
     CDS             1628..2374
                     /locus_tag="OQM83_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCS1520758.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2374..4470
                     /locus_tag="OQM83_002518"
     CDS             2374..4470
                     /locus_tag="OQM83_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCS1520759.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4516..5655
                     /locus_tag="OQM83_002519"
     CDS             4516..5655
                     /locus_tag="OQM83_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCS1520760.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5643..6089
                     /gene="etp"
                     /locus_tag="OQM83_002520"
                     /gene_synonym="yccY"
     CDS             5643..6089
                     /gene="etp"
                     /locus_tag="OQM83_002520"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCS1520761.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            6109..8289
                     /gene="etk"
                     /locus_tag="OQM83_002521"
     CDS             6109..8289
                     /gene="etk"
                     /locus_tag="OQM83_002521"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCS1520762.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8404..9702)
                     /gene="appA"
                     /locus_tag="OQM83_002522"
     CDS             complement(8404..9702)
                     /gene="appA"
                     /locus_tag="OQM83_002522"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCS1520763.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9782..9874)
                     /gene="cbdX"
                     /locus_tag="OQM83_002523"
     CDS             complement(9782..9874)
                     /gene="cbdX"
                     /locus_tag="OQM83_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCS1520764.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(9887..11023)
                     /gene="appB"
                     /locus_tag="OQM83_002524"
     CDS             complement(9887..11023)
                     /gene="appB"
                     /locus_tag="OQM83_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCS1520765.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(11035..12579)
                     /gene="appC"
                     /locus_tag="OQM83_002525"
     CDS             complement(11035..12579)
                     /gene="appC"
                     /locus_tag="OQM83_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCS1520766.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12713..13570)
                     /locus_tag="OQM83_002526"
     CDS             complement(12713..13570)
                     /locus_tag="OQM83_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCS1520767.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13627..13965)
                     /gene="hyaE"
                     /locus_tag="OQM83_002527"
     CDS             complement(13627..13965)
                     /gene="hyaE"
                     /locus_tag="OQM83_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCS1520768.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(13962..14549)
                     /locus_tag="OQM83_002528"
     CDS             complement(13962..14549)
                     /locus_tag="OQM83_002528"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCS1520769.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14546..15252)
                     /gene="hyaC"
                     /locus_tag="OQM83_002529"
                     /pseudo
     CDS             complement(14546..15252)
                     /gene="hyaC"
                     /locus_tag="OQM83_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(15271..17064)
                     /gene="hyaB"
                     /locus_tag="OQM83_002530"
     CDS             complement(15271..17064)
                     /gene="hyaB"
                     /locus_tag="OQM83_002530"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCS1520770.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNTSPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(17061..>17395)
                     /gene="hyaA"
                     /locus_tag="OQM83_002531"
     CDS             complement(17061..>17395)
                     /gene="hyaA"
                     /locus_tag="OQM83_002531"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCS1520771.1"
                     /translation="KMGCKGPTTYNACSSTRWNDGVSFPIQSGHGCLGCAENGFWDCG
                     SFYSRVVDIPQMGTHSTADTVGLTALGVVAAAVGVHAVASAVDQRRRHNQQPTETEHQ
                     PGNEDKQA"
BASE COUNT         4285 a         4615 c         4480 g         4015 t
ORIGIN      
        1 aagttcaggt ccatatttcc gcattcactc cccgcgacgc agaagtgctt ataccaggat
       61 tacgtgtgga attttgccgc gtaaatggcc tgcgaggacc aacagcggca aatgtttatc
      121 tctcttaata tgacgctgct tgtttaaagc tgactggttt tattggaata gtgcaatagt
      181 caacatcata cattataata tcagttactt ttccacaaag aaactaatcc atttaagcga
      241 atatctctgc acctaatagt tataaatgcc atttaacagc cagttatagt accacttgaa
      301 actatattac caacaataat taatagcttt cgtaacaatt tccttatctt taatttgtat
      361 tttgctaaga tttatcttat gctcacaagt cgtatttcca gaggggatcg ttaataatct
      421 tatatttccc tctgcgcacc tttcctaccc gcagtatcag tatatagtgc aagcggtatg
      481 aggaaaggga gatttcacaa ggatgttata ctcaaagtag acaatcctga tctcaagcgt
      541 acgtattgtc catgcgatga ataaaggaaa agttatgaaa cacaagcttt ctgcaatcct
      601 tatggccttc atgttgacga cgccagctgc gtttgctgcc tctgaagcaa caaacgggac
      661 cgaggcaacg acgggtacta ctggcaccac aacgaccact accggcgcaa ccacgactgc
      721 tactaccact ggtggcgtgg ctgctggcgc tgtcggcacg gcgacagttg tcggtgtggc
      781 aacggctgtt ggtgtggcga cgttggcagt tgttgcagca aatgacagtg gcgatggcgg
      841 ttctcataac acctcaacca ccacgtccac tacacgctaa tactgcttta acgcgtcgaa
      901 attaaagtta aatgataccc ggttgcgata ttcgtaccgg gctttagaga tttgcatttt
      961 tttaattaac ctttcaagga ttaaaggtgc gccctcttat tttatcgatt ttcgcactat
     1021 ttcttgcggg atgtacgcac agccagcaaa gtatggtcga tacatttcgc gccagccttt
     1081 tcgataatca ggatatcacc gtagcggatc agcagatcca ggcgttgcct tattccacga
     1141 tgtatttacg ccttaatgaa gggcaacgaa tctttgtggt actgggatat atagaacaag
     1201 aacaaagcaa atggttatcc caggataacg ccatgctggt tacccacaat ggacgtcttt
     1261 taaaaaccgt caaacttaat aataatctgc tggaagtgac aaattccggg caggaccctc
     1321 tgcgtaacgc gctggcaata aaagatggca gccgctggac gcgcgatatt ctctggagtg
     1381 aagacaacca ttttcgctct gcgaccctga gttctacttt ttcctttgct ggattagaga
     1441 cgctgaatat tgcgggtcgc aatgtgctgt gtaatgtctg gcaggaagag gtgacttcca
     1501 cgcggccaga aaaacagtgg caaaacacat tctgggtcga ttcggctact ggccaggttc
     1561 gtcaaagtcg gcaaatgtta ggcgcagggg ttattcccgt agaaatgacg tttcttaaac
     1621 ccgcaccatg aataaattac agtcgtattt cattgccagc gtactttacg taatgacacc
     1681 ccatgccttt gcgcaaggaa cggtgactat ttatctgcct ggcgaacaac agacactttc
     1741 cgttggcccc atggaaaatg ttgtccagtt ggtgacacaa ccgcaactgc gcgatcgcct
     1801 ctggtggcct ggtgccttgc tgaccgactc tgcggcgaaa gctaaagcgc ttaaagacta
     1861 ccaacatgtc atggcacagc ttgcttcctg ggaagcagaa gctgacgacg atgtggcggc
     1921 aacgattaaa tccgtgcgcc agcagttact caatctcaat atcactggtc gcttaccggt
     1981 caaactggac cctgattttg tacgagtgga tgagaacagc aatcccccgt tggtcgggga
     2041 ctatacgctc tataccgtac agcgtccggt gactattact ctgctgggtg cggtttctgg
     2101 ggccgggcaa ttgccctggc aggcaggccg tagcgttacc gattatctgc aagatcaccc
     2161 ccgccttgca ggcgcggaca agaataacgt tatggtgatt acgcctgagg gtgagactgt
     2221 cgtcgcccct gttgccctgt ggaataaaag acatgtggaa ccaccgcctg gcagccagct
     2281 ttggttgggc ttttctgcgc atgtactgcc cgaaaaatat gctgatttaa atgaccagat
     2341 tgtgtctgtt ttaacgcaac gtgttcctga ctgatgaaga agaattctta tcttttaagc
     2401 tgcctggcca ttgccgtctc cagtgcctgt catgctgaag tattaaccta cccggatccg
     2461 ctgggttcgt cgcaatcaga ctttggcggc acaggattgt tgcagatgcc aaatgcgcgc
     2521 atcgcaccgg aaggtgaatt cagcgtcaac taccgggata acgatcaata ccggttctac
     2581 tccacctccg tggcgctgtt cccatggctg gaaggcacca ttcgttatac ggatgtgcgc
     2641 acacgcaaat atagccagtg ggaagatttc agcggcgatc agtcatacaa agacaaatca
     2701 ttcgatttta aacttcgcct gtgggaagaa ggttactggc taccgcaagt ggcgtttggt
     2761 aaacgtgata ttgctggtac gggtctgttt gacggtgagt atctggtggc cagcaagcaa
     2821 gcggggccat ttgatttcac cctcgggatg gcatggggct acgccggtaa tgcgggcaat
     2881 attaccaacc cgttttgccg ggtgagcgat aaatattgtc atcgcgcaga gtctcacgat
     2941 gcgggcgata tcagctttag cgatatcttt cgtggcccgg cttccatctt tggcggcatt
     3001 gagtatcaaa cgccgtggaa tcccctgcgt ctgaaactcg aatacgatgg caacaattac
     3061 cagaatgatt tcgctggcaa actgcctcag gcaagccatt tcaacgtcgg cgcagtttat
     3121 cgcgctgcca gctgggcaga tctcaacctg agttatgaac gcggtaacac gttgatgttt
     3181 ggcttcacgt tacggaccaa tttcaacgat ctgcgccctg ccctgcgcga tacgccaaaa
     3241 ccggcatatc aacctgcgcc tgaatctgaa ggattgcagt acaccacagt agcgaaccaa
     3301 cttaccgcgc tgaagtacaa cgcaggtttt gaagcaccgg aaattcagct gcgcgataag
     3361 acgctgtata tgtctggtca acaatacaaa taccgtgatt ctcgcgaagc ggtcgatcgt
     3421 gccaaccgga ttctggtgaa taacctgccg caaggcgttg agaagattag cgtgacgcaa
     3481 aagcgcgagc atatggcgat ggtgactacc gaaaccgacg tagccagcct gcgcaaacag
     3541 ctggctggta cagcgcctgg tcaatcagag caacttcaac aacaacgtgt tgaagcagaa
     3601 gatctttctg cctttggtcg gggctaccgt attcgtgaag atcgctttag ctactctttc
     3661 aacccaacac tttcacagtc gctgggcggc ccggaagatt tctatatgtt ccagctgggg
     3721 ctgatgtcca gtgcccgcta ctggtttacc gaccacctgc tgcttgatgg cggtattttc
     3781 accaatattt acaacaacta cgacaagttt aagtcttcgc tgttgcccgc ggactctacc
     3841 ctgccccgcg tgcgcacgca tatccgtgat tacgttcgca atgacgttta tctcaacaac
     3901 ttgcaggcga actactttgc cgacttaggc aatggtttct atggccaggt gtatggcggt
     3961 tatctggaaa cgatgtacgc cggtgtcggt tccgagctgc tttatcgccc gctagatgcc
     4021 agctgggcgc tgggtgtgga cgttaactac gtgaagcaac gtgactggga caacatgatg
     4081 cgcttcaccg attattccac gccaactggt ttcgtgacgg cttactggaa cccgccgacg
     4141 ctcaatggcg tactgatgaa acttagcgtt gggcaatatc tggcaaaaga taaaggggca
     4201 acgatcgacg tcgccaaacg ctttgacagc ggcgtggcgg taggggtatg ggcggcaatc
     4261 agtaacgtat ctaaagatga ctacggcgaa ggcggcttta gtaaaggttt ttatatctcg
     4321 attccgttcg acttgatgac cattggacct aaccgcaacc gcgcggtggt ttcgtggaca
     4381 ccattgacgc gtgatggtgg acaaatgctg tcacgcaaat accagctcta tccaatgacg
     4441 gcagagcgag aagtaccggt tggacaataa cctgacgtaa ttcataacat tacattgact
     4501 gataaatata aaccaatgaa aaagaacatt tttaagtttt cggtactgac attggctgtt
     4561 ctgtctttga cagcatgtac ccttgttcct ggtcagaatt tatcgaccag taacaaggat
     4621 gtcattgaac tacctgacaa tcagtatgac ctggacaaaa tggtcaatat ctatcccgtc
     4681 acgccaggat tgattgatca gttgcgcgcc aaaccgatta tgtcgcaggc taacccggag
     4741 ctggagcagc aaatcgcaaa ctatgaatat cgtatcggga ttggtgatgt gttgatggtt
     4801 acggtctggg atcacccgga actaaccacc cccgcagggc aataccgtag cgccagtgat
     4861 accggcaact gggttaacgc cgatggtgcg attttctacc cctatattgg tcgcctgaaa
     4921 gtggccggga aaacacttac ccaggtacgc aacgagatta cagcgcgtct cgattcagtc
     4981 attgaaagcc cgcaagtcga tgtcagcgtc gcggcgttcc gatcgcaaaa agcgtacgtc
     5041 accggcgaag tcagcaaatc gggtcagcag ccgattacca atattcctct gaccatcatg
     5101 gatgcgatta acgccgcggg cggtctgacc gcagatgcag actggcgcaa cgtggtgctc
     5161 acccagaatg gcgtaaagac caaagtgaac ctctatgcgc tgatgcagcg tggcgacttg
     5221 cgccagaaca aactgctgca cccgggcgat attttattta ttccgcgcaa cgatgatctg
     5281 aaagtcttcg tgatgggtga agtgggtaaa cagagcacgc tgaaaatgga ccgcagcggt
     5341 atgaccctgg cggaagcgct gggtaacgct gagggtatga atcaggatgt cgctgatgcg
     5401 acggggattt ttgttattcg tgcaacgcaa aacaagcaga atggcaaaat cgccaatatt
     5461 tatcagctga atgcgaagga cgcatcggca atgatactcg gaacggaatt ccagcttgaa
     5521 ccttacgaca ttgtttatgt cactaccgcg ccgctggctc gttggaaccg tgtgatttct
     5581 ctgctggtac cgacgatttc cggggtgcat gacctgacag agacttcacg gtggattcaa
     5641 acatggccca actaaaattt aactcaatcc tggtggtttg taccggcaac atttgccgtt
     5701 cgcccattgg cgagcgcttg ctgcgtaaac gtctgccggg cgtaaaagtc aaatcggcag
     5761 gtgttcatgg tctgataaaa caccctgcag atgcgacagc ggcagatgtt gccgctaatc
     5821 atggcgtatc tcttgaagga catgccggac gtaagctcac tgctgagatg gcacgaaatt
     5881 acgatctgat tctggcaatg gagtcggaac atattgctca ggttaccgca attgctcccg
     5941 aggtccgcgg gaagacaatg ctgtttgggc aatggctgga acagaaagag atcccggatc
     6001 cctatcgtaa aagtcaggac gcatttgaac atgtctacgg tatgttggag cgcgccagtc
     6061 aggaatgggc gaagcgcctc agccgataat ttgagtttat aaaatacgat gacaactaaa
     6121 aatatgaata cgccaccagg cagcactcag gaaaatgaga tcgatctgct tcgtctggtc
     6181 ggcgagttat gggatcaccg taagtttatt atcagcgtga ccgcgttatt cacgctgatc
     6241 gctgtcgctt actcgctgtt aagcacacca atttatcagg cagatactct ggtccaggtt
     6301 gagcaaaaac agggcaacgc cattctcagc ggcctgagcg atatgatccc taactcatcg
     6361 cccgagtctg caccggagat ccaactgctg caatcgcgca tgattctcgg taaaaccatt
     6421 gctgaactga atctgcgcga catagttgag cagaagtatt ttccgattgt gggtcgcggc
     6481 tgggcgagat taaccaaaga aaaaccaggt gagctggcga tcagctggat gcatattcca
     6541 caactgaatg gtcaggatca gcaactgaca ctcacggttg gggaaaacgg ccactataca
     6601 ctggaaggtg aagagttcac cgtcaatggt atggtcggcc agcgtctgga aaaagatggc
     6661 gttgcgctga ctatcgcgga cattaaggcc aaaccaggaa cacagtttgt cctgagccag
     6721 cgtaccgaac tggaagcgat taacgcattg caggaaacct ttaccgttag cgaacgcagt
     6781 aaagaaagcg ggatgctgga acttaccatg actggtgatg atccccagtt gattactcgt
     6841 attctgaaca gcatcgctaa caactatttg caacagaata tcgctcgcca ggcggcgcag
     6901 gattcacaaa gccttgaatt cttacagcgc cagttacctg aagtgcgcag cgagctggac
     6961 caggcggaag aaaaactcaa cgtttatcgc cagcagcgcg attcggttga ccttaacctg
     7021 gaagccaaag ccgttcttga gcagattgtg aacgttgata atcaactcaa tgagctgacc
     7081 ttccgcgagg cagagatctc ccagctgtat aagaaagatc acccaactta tcgtgcgctg
     7141 ctggaaaaac gccagacgct ggagcaagaa cgcaaacgcc tgaataagcg ggtatcggca
     7201 atgccttcca cccaacagga agtgttgcgt ttaagtcgtg acgtagaagc gggccgtgcg
     7261 gtatatctgc aattacttaa ccgccagcag gagttgagta tttcgaaatc cagtgccatt
     7321 ggtaacgtgc ggattatcga cccggcagtc actcagccgc agccagtgaa accgaaaaaa
     7381 gcgttgaatg tggtgcttgg ttttattctt ggcctgttta tttctgtggg tgccgtgctg
     7441 gcgcgtgcga tgttgcgtcg tggtgtagaa gccccggaac aactggaaga gcacggcatc
     7501 agcgtttatg ccactatccc aatgtccgag tggctggata aacgcacccg tctgcgtaag
     7561 aaaaatttat tttctaatca gcagcgccat cgtactaaaa atatcccctt cctggcggtg
     7621 gataacccgg cggattctgc tgtggaagcc gtacgtgcgc tacgaaccag tctgcatttc
     7681 gctatgatgg agacggagaa taacattctg atgatcaccg gtgcgacgcc agacagtggt
     7741 aaaacgtttg tcagttcaac tctggcagcg gtgatcgccc agtccgatca aaaagtgtta
     7801 tttattgatg ccgacttacg ccgtggttat tcgcataacc tgtttaccgt gagtaatgaa
     7861 catggcttgt cggaatatct ggcaggtaaa gatgagctca acaaagtgat ccagcatttt
     7921 ggcaaaggag gctttgatgt gattactcgc ggtcaggtgc cacctaaccc gtctgaactg
     7981 ctgatgcgcg atcggatgcg tcaattactg gaatgggcga acgaccatta cgatctggtg
     8041 attgtcgata cgccgccgat gctggcggtg agtgatgccg cggtcgtggg gcgttctgtt
     8101 ggcaccagcc tgctggttgc gcgttttggc ttgaacaccg ccaaagaggt gagtttgtca
     8161 atgcagcgtc tggaacaggc aggcgtcaat attaaaggcg ctatcctcaa tggtgtgatt
     8221 aaacgcgcca gcaccgctta cagttacggc tataactatt acggttatag ttactccgag
     8281 aaagagtaaa actgaaagtg gccggatgcg gcgtgaacgc cttttccggt ctacgcatta
     8341 gacagttctt cgtttgtcat cagcctcaga gcattcaggt aactgaatgc tcttttttat
     8401 gcattacaaa ctgcacgccg gtatgcgtgc ttcattcacg atttgcgtaa aacctgccaa
     8461 cgaacacatg ccctgcgcat ttcgctcttc acatcctgcc agggtcagtt tcacctctcc
     8521 gggcggcgta tttaatgaca gcggcgtttt atcacgcatc tgctgtaaag tctggaagac
     8581 cagcgaaacc tgaatccact ggctgttatc gcttagccga cgccagcgtt caaacaccag
     8641 ttcaccacct ggcggcgtgt tatccggctg accgggaagc gtccagttga gctccagtgc
     8701 gccgccgaga tttgccagat tagtatcgtg tccggcgata aacagcactg aagtgggtaa
     8761 tgtcacacca tacgcctgtt tttgcggtgg atggggcgtc aacgctgtct tgatcaaatc
     8821 taataacggg gtggcgcggc tgcgggcaac ctctggcgtg cgttgtagca aataaaattg
     8881 cgcgttatgc aaacttagca aggtgttcca ctggtgtgaa tcggtgatcc ttccccaccc
     8941 cggctccggc attccctgtg cttgttgcag gagaaatatc tccgtcagca ttgatgcgag
     9001 gcttaccgca ccggttaatg agacattgtc ggcgctcacc ttgagttccg atggtaatgc
     9061 ctgcgttaat gaacagcttt cgtcctgttt ctcacgttta aggcacaagt ttgattgcgg
     9121 aaaattaagc acccgttcca gttcgcgaaa cgccgtttgc cgatgcccgg taaagtcagc
     9181 aattgaccct cctgccctgc tgaggatcgc gtcagtcacg ttcgcgttat ccagttggca
     9241 aacgccagtt tttagaggat taaataacgg atcgggactg gacgtatcag cctgggtatg
     9301 tacggttatt gcacagtcag gtgccagccc ggcggcgaag gcttcgcctg ttttacgggt
     9361 acgctcgtcg acatcagcaa taatcgcgac ctgaccagac tgcgggcagc cctttttcgc
     9421 cagcaatccg tcggctacca gacgctggcg ttggtaatgt ccgagatagg cgattagctc
     9481 accaccgcgc ggtgtcagcc aacccagttt taccggccag gttggccatg cgtctggggt
     9541 gacatcctgc atcagttgcg tggccttggt tggagcacgc acaccatgac gactgacaat
     9601 caccacactt tccagcttca gctccggctc actctgagcg aatgcagatt gcggggttaa
     9661 cggaatcaga agagataaaa atgggattaa gatcgctttc atcgatatgt ttccgctttt
     9721 catatctgac attattgatt gcctgatgcg atgctaatgc gccgcatcag gcctacgttc
     9781 gttaactttt cagacgcggg tccagccata ccaacacaag ggtggagagc gaacacatca
     9841 acaaaatgcc gacgaaccaa agtaaatacc acattgttcc tgctccttag tacaactcgt
     9901 tttcgttacg gcggagagtt tctgttgtca tgcgtcccca cattttgtag tagctccaga
     9961 gagtgtagag caacacaatg ggcaaaaata tcagcacgat taccaacata atgctcagcg
    10021 tcagctgact ggaagtgctg tcccacaacg tcaggctgga gattggactc acgcttgacg
    10081 gcatgacaaa ggggaacagc gtgatgcctg ccgtgaaaat cacgccaaat tgcatcaatg
    10141 acgccattaa aaatccccaa cccgggcgac cacgataaat cgccatcacc gtcagcaatg
    10201 ggcagaagaa ccccagcagc gggaagatcc acaaaacggg cgattcgacg aaattattca
    10261 tccaggcacc aggtagcact gccaccagtt tcattaacgg attggaagga ccgttagcat
    10321 cctgggcgag cagtacaaag ccatcaatac cgacccacag ccagtaaccc gccagcaaaa
    10381 agcacagcat caccaacagt gcagcgcgtt tggtggccag ctgtgaacgc agatgaatca
    10441 caccaacagt tttcagttgt aaccagacgc caccttgcaa aatcaccatc ccaaggctga
    10501 gcaatccgca caataaaggg aatggcgtca gcagttgcca gaagctgccg agatactcca
    10561 cgcgtaattg cggtgtgaag gcaaacggca cgccgagcaa caagttgccg aaggcgatac
    10621 cgaagactac cggcggcacc agactgccga tgaccagacc ggcgtcccac attttacgcc
    10681 agcgtgcatc ggcgattttt ccgcgataat caaaggccag cgggcggaag aacagtgagc
    10741 acagcaccag gatcatcgcc acataaaagc cggaaaacgc cgctgcatac actctgggcc
    10801 aggcggcaaa taatgcccca ccagcgagga tcaaccagac ctggttgcct tcccagtgtg
    10861 caccaacgct gtttatcact atccggcgtt catcatcatt acgcgccacc agcggcagca
    10921 gacagccgat ccccatgtca aatccgtcgg agatcataaa gaccaccagg atcacgccaa
    10981 tcagcagcca ccagatgaag cgcaatgttt cataatcaaa catgattttc ccctttaccc
    11041 ctgttgctgc gtcggttgtt cactctgcat cgcgctcggc cccagacggg catatttctg
    11101 catcaggtag acttcggcga ttaagaacag ggtgtaaagc cctacgatca tgatcagtga
    11161 gaaagccagt tgtcctgtgg ttaaagcgga gtgcgcggag tatgtcggta agatgtcctg
    11221 tatcgcccac ggctgacgac caaactcggt cataaaccac ccggcttcaa tcgcaatcca
    11281 cggcaacggc aaactccaga gcgccatttt cagcacccag cgatgctggt cgattttgcc
    11341 acgcagcgtc tggacaagcg caatcagcat caccagtagc ggcagggaac cacagcccac
    11401 catgatgcgg aaactccaga ataccggcgc aacctgaggt atcgcgccac gcatcgccgc
    11461 ctggtactgt gcggctgtga catgattcat atccggcgca tagcgggaga gcaacatgcc
    11521 gtagcccagg tcaccttcca gttcccggaa tgcctgcaac acatgcggct cacgattgcc
    11581 ttgcgatatt tcctgcatta acagccaggc catacgtccg cgttgcaagc gtgggtaggt
    11641 ttcagccatc aaatttttca gacccggcac ggttttatct aatgagtgag tggcgaggat
    11701 ccctaacagc gcgggaattt tgatggcaaa ggcgttacgc tcttgatcct gttccggcca
    11761 ggcaaccaca tggaacggtg caggagcagg ttccgtttgc cactcccctt ccatcgccgc
    11821 cagttttacc ggttgtactt gcgcgacttc atacgcagaa ctgtctccga gttgtagcgt
    11881 gccgataatc gccagagtac cgaagacgga accgatggca aacgagcgta atgcaacatc
    11941 gcgctcccgt ccgcgcagta aataccaggc gctgatcgcc ataataaaca tggccccggt
    12001 cacgtagccc gccattacgg tgtgcacaaa tttcacctgg ctgaccgggt taaagaccag
    12061 ctcgctgaag ctggtcatct ccatacgtag ggtgtcgata tcaaaatgcg caccggtcgg
    12121 gtattgcatc caaccgttgg cattcaatat ccacaatgca gagagatttg aaccgaacgc
    12181 caccagccac gtcaccagca ggtgctggta tttattcagc cgctgccagc cgaagaagaa
    12241 aagcccgaca aaggtggatt cgaggaagaa ggccattaat gcttccatcg ccagcggtgc
    12301 gccaaaaata tcgcccacat agttggaata gaatgaccag ttagtaccaa actgaaactc
    12361 catggtcagg ccggtagcca cgccaagagc aaagttgata ccgaagagct taccccagaa
    12421 gcgcgtcata tcgcggtaga ttgttttgcc ggtgaccacg taaatggttt ccatgatagc
    12481 cagcaaaaaa atcagcccca gggtaagggg tacaaataaa aagtgataca gcgcggtcag
    12541 agcaaactgc cagcgcgata aatcaatgac atcccacatg cgcactcctg taggcgaaga
    12601 gagaaaaata acccgataat gttagatctc cctctttatc ctgaaactct acgaccagag
    12661 gtattcgccg attgcgatat tcataagact gaaagcgata cttaccgtct ttttacgtcg
    12721 gtgcagcttc cgccagccac tgacatacct cgctaagccg ctgcgcagag tcgaccaaat
    12781 cttctggcgc tgccagcacc acttccggta ttgggcagat ttcataactt tccagtaaag
    12841 gaccttttaa ggtgtccgta cagcgtaaat gccagacatg gcgtaacccc gtggcgttga
    12901 tatagctctc gccatagcca atggtacgaa tctgaatatt tcccggccca cagagacgtg
    12961 agagaaaaag ccgatcagcc tcgctgatgg gtaattgcgt cagattaatg ctgtgcggct
    13021 gcgcgtcggg gttacgtaca tgcgccagta actcatgcgc caacggtagg ccattcatca
    13081 ggccatcaat gggcggcggt aacagcgaat ctgtcggcaa gagattttgc gtggccgcct
    13141 gccacaacgc cagcggcgcg cagccggcct ccagtttgtc ctccagcaac ttttcgccgc
    13201 gacgtttgcg cacccgccat aatccgcaga agatcgcctc ctgtatttca ctttcgctgt
    13261 cgtcagcctg ctgaatgcgc actgacactt ccccttcccc gagaagcgta ttgataaagt
    13321 gacgatcgtc ggcgttgaga tttaacagat cgactgccag cacctcgccc aacggcggct
    13381 ggcgttgact tagttgatgt tgtaactcgt ttaacagcgc aatcacctgc gggctgtgag
    13441 cacattgctc cagggccgcc atactcggtt catcattcac ctgacagctg atcggcagtg
    13501 gattcatgct gaaactgtca tcgttcggtt gcgttcctgg ccccagcaga tggaaaaaag
    13561 tttcgctcat gaggcacgct cctgttgtgg ttcgacaagc ccacgcatca ggtttatcag
    13621 ttccgctcac gggtgaatac cattcagcac accgcgatag tttccgccgg taaacactaa
    13681 tgtggcggga aagcgaaaga cgccaaagcg atctccgatg gcttcgctct gttcaaggtc
    13741 agcaatcgcc acctgccatg tgtagccggg aaactcgcgc agtaattcgc caatcattac
    13801 cggattatcg ctgacctctg gcgtgcgttt cgggtcactg cttaataaca ccacgccgtc
    13861 tggcgcttgc gtaagccagt cgtcaagacg gaattcacta accggcgtcc agccgcgcgc
    13921 cagcattcgt tgccacaacg catcaaatgg cgtgtcgttg ctcatccctg ctcctccagt
    13981 gtcatcctgt actggcgcaa gcgaacgcct tcgtaatttt ccatcgacag acagtcataa
    14041 ttgagacagc gcgattcatt agccggttgc ggcacaattc cccatgcagc cagctgcgcc
    14101 agcgccgcct gttccgctgc gggcagttgc tcccgtgcca gttcgctcag gctgccgccg
    14161 tagtcgtcga gcattgcggg ttgcagaccg acgagggcaa tatgtgctgg cagatgtccg
    14221 cggatatctg ccagcgccag cacttcggag aaactgttct ggtgcaggct cattttcttc
    14281 gcgctgagat aagccggaat acgttctccg gcataggttc gcagcgttcc aggttccagc
    14341 ccgtagtcaa tggcatcgag aatcaacaga tggctggcgc tttcgacata ccccagcaag
    14401 ttcagtccct gagtaccgcc atcgacaatc tccacatact cgggccagtg gtaatgggca
    14461 tacaaccgtt ccgccacccg cacgccgaaa ccttcatcgg cccacagcag gttgcccagc
    14521 cccattacca caacgcgttg ctcgctcatg aacgctcctt gttacttatt ttgccaaatt
    14581 tgtggctacg gtagccgttg accatggtgg agatcaccgt gtcgtcggac atgatgtctt
    14641 cacgcagcgc catatagaca tgaccgatca caaacgcgcc aatcagccac atccccagcc
    14701 gatgccagct gtgaatgtcc attgagttgc ctcccgtcca gtagaaaaat tccaccacat
    14761 aacggaacgg cgcaaaaata gcgtactggc tgtgttcgct gtacagcgca aagccggtga
    14821 tgatcataaa gaccgacatc aggaaatagc cgaacatcgc cgcctgggcg atggggttat
    14881 ggcctatatc ggcactcgga cgttttgcca gaacagatac cagcggattt cataccacac
    14941 gccctgccac cagcttttac gccataccgg cacgataaac agctcgcggg agtatcgatt
    15001 gccaacaaaa gcccagtaga tccgcatcag caagaccacg gtaaaaacca tcccggcgct
    15061 gaagtgaatt aacctgatgt agcccatata gaacagatac gtcgcctcgc cgctgacgga
    15121 aggtagcggc ttgccgataa agtatccggt gaccatcaac accgccatgc ataacaccgt
    15181 cagccagtgc cagatgcgga ctggcgcttc aaagacatag tggctgacaa cgttgtcgct
    15241 tttctgttgc atgatgattc tccttcgctg ttaacgcacc tgcacggaga tcagctcgct
    15301 accgtcgtcg cccagcacgt gtgttgaaca ggcgaggcac gggtcaaagc tgtgcagagt
    15361 acgcaggatc tccagcggtt gctcggggat cgccattttg gtgttcatca gcgccgcttc
    15421 ataagcgcca atctgccctt tgggatcgcg cgggctggtg ttccaggtgg tcggcaccac
    15481 gcactggtag agatcaatct tgccatcgcg aatggcggcc cagtggccta acgccccgcg
    15541 cggcgcttcg gtaaagccga cgccacggca ctctgtcggc caggttgcag gttcccattt
    15601 ttccgtggag gcggtggcaa gattgccgtt tttcaggtta gtcatcagct tgttgaagaa
    15661 atattgcaac ttacctgcgg cccactgtgc ttcgtgcgcg cggcacaaaa tgcggcccaa
    15721 cgttgactgg ataccggaaa gcggcaggtt cagcgccgac atcatgcgat cgaccgactc
    15781 aacggtcgca gcatcgcctt tgtgataagc gattaacgtg cgtgccagcg gccccacttc
    15841 catcgcgtta ccgcgccagc gcggcgcttt gatccacgag tagcgttcct gttcattcag
    15901 ctgctgaatg ttggtatcgc tgcctttgac atcgccgggg ttgtaccacg ggtcggtaat
    15961 gccatcgaat ggatgacgcc cgacctgatc gttgggataa cgataccagg cgtggtcgac
    16021 aaactcctgc acctgctgcg gatcaaccaa atccactggc agcacattgt tgaagtcgcc
    16081 gttaatcacc gcgccgccag gcatcagcag acttttctca ccaaagtcgt tggcaatatc
    16141 cgggaacgca ccgtagctga ggacacattt atcagaaaga cccgtgccga tttcgctcca
    16201 cggtttgttg aactgaccga tggctaaggc gtcggggatc atcacgttgt taatgaagtc
    16261 cgccgtgcgg gtgataatcg actgcaccag gttcaggcgt tccatattga ccgccccgac
    16321 cgcgccgctt tcgtcaatgt taatggcgca aggcatcccg ccgacaatcc agtttggatg
    16381 cgggttttta ccgccaaaga ccgcgtggat tttgacaatt tcacgctgga aatcgagagc
    16441 ttcgagatag tgggcaaagc ccatcaggtt agcttctggc ggcagtttgt actgcgggtg
    16501 cccccagtag ccattgcgga agatccccaa ctgcccgcct tcaacaaatt ttttcaggcg
    16561 gttttgtacg tcgaagaaat agccagggga tgatttcggc caagaggaga gactttgcgc
    16621 cagttcggag gttttccgcg ggtcggcttt cagcgcatct aacacatcga tccagtccat
    16681 cccggcaagc tgatagaagt gcaccagatg atcgtggcac cagagcgttg ccagcataat
    16741 gttgcggatg atattggcgt tgtccggcac tttaataccg atagcatctt cgatggcgta
    16801 aaccgaagcc agggcgtgta cgccagtaca gacgccgcag atacgttcaa cgaacgccca
    16861 cgcatcgcgc gggtcgcgcc cttgtaggat gatctccagc ccgcgaaaca tggtgccgca
    16921 ggagacggca ttggtgatca cattctgatc gttaatattc acttcgcagc gcatgtggcc
    16981 ttcgatgcgc gtaatcgggt cgaccaccag gcggcgtccg gcattattga tggtgtatcc
    17041 ctgagtttcg tactgagtgc tcatgcctgt ttatcctcat tgcctggctg atgttcggtt
    17101 tctgtaggtt gctggttatg acgtctgcgc tggtcaacgg cgctggcgac tgcgtgcaca
    17161 ccaacagccg ctgccaccac accaagcgcg gttaaaccga cggtatcggc ggtggaatga
    17221 gtacccattt gcggaatatc gaccacgcgg ctgtagaacg aaccgcaatc ccagaaacca
    17281 ttttccgcac agcccaggca gccgtgacca gactggattg ggaaagaaac gccatcattc
    17341 cagcgtgtgg aggaacaggc gttataggtg gtaggccctt tgcagcccat tttgt
//
LOCUS       DAJVQG010000086        17293 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJVQG010000086 DAJVQG010000000
VERSION     DAJVQG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17293
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.086"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            92..1132
                     /locus_tag="OQM83_002532"
     CDS             92..1132
                     /locus_tag="OQM83_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCS1520772.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="OQM83_002533"
     CDS             complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="OQM83_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCS1520773.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETMSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2350..4509)
                     /gene="aas"
                     /locus_tag="OQM83_002534"
     CDS             complement(2350..4509)
                     /gene="aas"
                     /locus_tag="OQM83_002534"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCS1520774.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5095..6126
                     /gene="galR"
                     /locus_tag="OQM83_002535"
     CDS             5095..6126
                     /gene="galR"
                     /locus_tag="OQM83_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCS1520775.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="OQM83_002536"
     CDS             complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="OQM83_002536"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCS1520776.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7517..8452
                     /gene="lysR"
                     /locus_tag="OQM83_002537"
     CDS             7517..8452
                     /gene="lysR"
                     /locus_tag="OQM83_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCS1520777.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLLTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8439..9086)
                     /locus_tag="OQM83_002538"
                     /pseudo
     CDS             complement(8439..9086)
                     /locus_tag="OQM83_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase"
     gene            complement(9217..10635)
                     /gene="araE"
                     /locus_tag="OQM83_002539"
     CDS             complement(9217..10635)
                     /gene="araE"
                     /locus_tag="OQM83_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCS1520778.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10950..11711)
                     /gene="kduD"
                     /locus_tag="OQM83_002540"
     CDS             complement(10950..11711)
                     /gene="kduD"
                     /locus_tag="OQM83_002540"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCS1520779.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11776..12612)
                     /gene="kduI"
                     /locus_tag="OQM83_002541"
     CDS             complement(11776..12612)
                     /gene="kduI"
                     /locus_tag="OQM83_002541"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCS1520780.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(12899..14080)
                     /locus_tag="OQM83_002542"
     CDS             complement(12899..14080)
                     /locus_tag="OQM83_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCS1520781.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            14335..15564
                     /locus_tag="OQM83_002543"
     CDS             14335..15564
                     /locus_tag="OQM83_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCS1520782.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            16024..16656
                     /locus_tag="OQM83_002544"
     CDS             16024..16656
                     /locus_tag="OQM83_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1520783.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            16990..>17293
                     /locus_tag="OQM83_002545"
     CDS             16990..>17293
                     /locus_tag="OQM83_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1520784.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPREDS"
BASE COUNT         4581 a         4511 c         3976 g         4225 t
ORIGIN      
        1 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
       61 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
      121 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
      181 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
      241 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
      301 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
      361 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
      421 ccgcgaagcg ctgcatgaca gcctcaagcg cctacacact gattacctcg atctttatca
      481 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
      541 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc
      601 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
      661 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
      721 gttaaaccgc agttttgaag taggtctggc agaagtaagc cagtatgaag gggtcgaact
      781 gctggcctat tcgtgcctag gtttcggcac gctgaccggg aaatatctca atggtgcaaa
      841 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
      901 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggcctag accctgctca
      961 gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
     1021 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
     1081 attagctgaa attgaagcgg tgcatcaggt ttatacttat ccggcaccat aagattaacg
     1141 ccggttaaaa ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt
     1201 gccagcgcaa acagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctaccatt
     1261 accgccagcg agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt
     1321 actgcaatcg cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg
     1381 agcggaacga caaaaaagcc ccccatcacg ccaatcagca tcaacaaggc ataggctggc
     1441 agcagctcgt gttgcaggga aaaaatcagt accaccacgc caatcaaaat cccggctggc
     1501 atacagcgtg acatggtttc cagcgtaact aacttcgctg ccgcacctgc gccaaccacg
     1561 ataccaatcg ctaccatcgc gttgagatag gtgggcgtag cgttatcggt aatgcccagc
     1621 gccaccggta cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa
     1681 ctggtgccca ccagcgaaaa acgcgtttca ccattgcgcc atagcgaggt gcaggcattc
     1741 aggaaactgc gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt
     1801 ttgggaatgt agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg
     1861 gcgacgagga catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc
     1921 gctatggtag aagcttccat taaaccgtta gctttcacta acttactacc cgtggttaat
     1981 tcgccgagaa taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta
     2041 tagccgagaa acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg
     2101 ttggcaaaca tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc
     2161 gcaaaaagaa tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga
     2221 tagaactgcg ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca
     2281 gagagaaact gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg
     2341 tgcactgact cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa
     2401 gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac
     2461 agcaagctcc ggtacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc
     2521 gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt
     2581 ggcatggact ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac
     2641 catttcgcct gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg
     2701 ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac
     2761 attctcggcg gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat
     2821 tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg ataccaggga ccgacaacag
     2881 gcgcgcatcc attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac
     2941 gttgatagaa acgacaggcg cgcattcggt cacgccgtag ccttcaagga tgcgcaggcc
     3001 aaatttatcc tgccaaagct gtttggtact ttcttgtaat ttttctgcgc ctgccaccac
     3061 atagcgtaga cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt
     3121 cgaggtgccg aacaacacgg tgcaactgcg gtcatacacc aactccggca caatacggta
     3181 atgcagcggg cttggataaa gaaacacttc tgcacctgtg agcagtggcg taaacaggcc
     3241 taccgtcagc ccaaaggagt gaaacagcgg taacgccgac ataaagcgat cgttggtggt
     3301 gaagtcggca atcgttttaa tctgctcgac gttcgccaga atgcttttat ggctatggac
     3361 gacgcctttc ggatggcctt cagaaccgga ggtaaagagg atcagcgcct cttcttccgg
     3421 ctgctgtttt acctgtgcca gacgtggcat cagcagatgc gcgaagatcc atactttgtc
     3481 ggcagtggtg acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg
     3541 ctccggcaga tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt
     3601 gatttcagcc gccgtaatag cactggtcag cccttttacc ccggcagtgt agttcatcat
     3661 tgcgggaatg cggcgacggg cgatggcccc aaaaatcact gccgcactga tgcctgcatt
     3721 gggcagcatt aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac
     3781 aaacagcgtt ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac
     3841 acatttcttc ccggctccga agcggtacat tgcactcagt aaagattcgt acagcgtttc
     3901 acgcgggcgc accgccattc gcgcttccat cattatttga tgcagcattt cgccagcgat
     3961 tttgcgacgg tcacgggcac gcggcgcatc cggcatctcc acctgcgttg gtggcaaaat
     4021 atgcagagta atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg
     4081 cgtaagttcc gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac
     4141 aaaacccgcg ccatcgtaga ttttcatcag cgagcctgtg gtggtgatgc gtccttcagg
     4201 gaaaatcacc actggtcggc cctgttccac cagacgtacc agatgtttaa tcgccatagg
     4261 ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg
     4321 ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa
     4381 aatgccatca ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc
     4441 ctgggtgtca cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct
     4501 aaaaagcatt ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac
     4561 gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca
     4621 ggttggtgca agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc
     4681 tgggatcttg attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg
     4741 aagggaaatg caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcaa
     4801 aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact
     4861 tcacctatca atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagca
     4921 gtcaatcgca acagcatttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct
     4981 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccgcccct tgaaccaacg
     5041 ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg
     5101 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
     5161 aataattcac ccaaagccag cgaagcgtcc cggctggctg tgcatagtgc aatggagtct
     5221 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
     5281 ggtctggtcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
     5341 caggtggctt atcacaccgg taatttttta ttgattggca atggttacca caacgaacaa
     5401 aaagagcgtc aggccattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
     5461 gccaaaatga tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg
     5521 ctgatcaacc gtatcctgcc tggcttcgaa aaccgttgta ttgctctgga cgatcgttac
     5581 ggtgcctggc tggctacgcg tcatttaatt caacaaggtc atacccgcat tggttatctg
     5641 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
     5701 gctgaaagtg gtattgcggc caatgaccgg ctggtgacat ttggtgaacc agacgaaagc
     5761 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
     5821 tgttataacg attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat
     5881 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt
     5941 ccgcgcctga ccaccgtgcg ttacccaatc gtgacaatgg cgacccaggc tgccgaactg
     6001 gctttggcgc tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg
     6061 ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
     6121 gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
     6181 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctgttgta
     6241 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacccgc
     6301 cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc
     6361 gcccgattca cataatggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga
     6421 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
     6481 cagatcgttg aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
     6541 ccgcacctga gtaattaaca cgccagactg cgctaccaga aagcgacccg gttcaatttc
     6601 cagtttcaca gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc
     6661 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc
     6721 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgta ccatagcacc
     6781 acacacctgt tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
     6841 cagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
     6901 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
     6961 cgggttaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat
     7021 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
     7081 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
     7141 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
     7201 cattaatcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa
     7261 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
     7321 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
     7381 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
     7441 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatata gattaccgga
     7501 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
     7561 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
     7621 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggcta aaattgtttg agcgcgtacg
     7681 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
     7741 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
     7801 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
     7861 ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
     7921 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
     7981 tgcgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg
     8041 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat
     8101 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattga ctgaacatca
     8161 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
     8221 ggcgggggta ggtgtttcgg tggttaaccc actcaccgca ctggattatg cggcaagcgg
     8281 tttagtggta cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
     8341 gcaccgcccg tcatcagcgc tggttcaggc gtttagtgag catttacaag cggggttacc
     8401 gaaactggtc acttctcttg acgcgatttt gtcgtcagct acgacagcat aaaagcgaca
     8461 gcatcctcgg catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctgga
     8521 accaataaac caatttctgt gcagccaaaa atgacaccct gcgccccctg ttctgcaagg
     8581 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
     8641 tcttcaaaaa taatctgatt aatcttcgcc cgttcatccg cttccggaat aagacaattg
     8701 atggaaaatt attccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
     8761 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgcc
     8821 actttatgca tcgtattggt acataacaca ataccttctg cgcccgcccg ctgtaagcca
     8881 agcgccgcct cagccagaat gtccccggtt ttatcccatt ccccgcgacg ctggcactct
     8941 tctatttcat gaaaatcgac gctatgtagc agcacttgcg cagagtgaag cccaccaagc
     9001 cgctgtttaa tgccttcatt tatcagacga tagtaaggaa tggtggattc ccagctcatt
     9061 cctcccagca aaccaattgt tttcattcct ctctctcctg atgtgtgtta ccgcaacaag
     9121 gctgaataag gaacgagaca aacgcctcaa cggccaggtg cccaatctct attaacgaaa
     9181 aaagggccgg atgtacagca catccggccc gtgaaatcag acgccgatat ttctcaactt
     9241 ctcgcctgcc atcagtttgc gttcgatatg ttccagcgtg acatttttgg tttccggaat
     9301 gagccagaaa gtgatgccca caaacgcaat gttcagcgca gtgtagagcc agaacgtacc
     9361 ggcagcacca atgctgtcga gcagcgtcag gaaggtcgcg ccgataatca tattcgacac
     9421 ccagttcgtg gtggtcgaac aggtaatacc gaaatcgcgg catttcagcg gctgaatttc
     9481 agagcacagg atccacacca ctggcgcggc gctcatcgca taaccggcaa tacacatcat
     9541 cgtcatgcca acagagagcc aggacaagcc actggaagcc gtaccgttat caaactgcat
     9601 caggcaatag cccagcacca gagtgcctaa cgccatcacg ctgaaaccaa ttttcagagc
     9661 cggtttacgc cctgctttat ctaccgtaaa caccgcaata aaggtggcga acataaaggt
     9721 cagccctacg accagagtcg caatcatctg ttgttctgtg gtcgtaaagc ccgccatttt
     9781 gaagatacgc ggcgcgtagt acatgatgat gttcataccg gtaaactgct gcatcgcctg
     9841 caacaacata ccgagaaaca cagcacgacg gacgttacgg ttgatcttaa acagtgccca
     9901 accgccctgt tttaacttca ggctttcacg aatttcgttg agttcttctc gcgctttttc
     9961 cgacgtatcg cgcagcatac gcaacacttc ttccgcctca atatgacgcc ccttttccgc
    10021 cagccagcgc gggctatttg gcaggaagac caccagaata atcagcagga ctgctggtaa
    10081 agcaagaacc cccaacattg cgcgccagtt accgctataa ctgaacgctg tatcggataa
    10141 aaacgccagc acgatgccga gtgtgaccat caactggtac atactgatca tcttaccgcg
    10201 aacgttttca cttgccattt cagaaagata cagaggagcg gtgtaagacg cgatcccgac
    10261 agcaatgccc agcaccaccc gagcggcgat taacatctct acgctggtcg caaaagcgga
    10321 ccctatagaa ccgagtacaa acaggatggc ccccgccatc aggctgtatt tacgccccag
    10381 gcggaacgac agccaaccat taaacagcgc accaattgct gcgccgagca tcatgctact
    10441 aaccacccat tcctgcaaac gactggtcag cacaaagtga tcggtaatga acggcaacgc
    10501 tccggcgatt acgccgatat caagaccaaa taacaatcct gcgaccgcag cagctaccga
    10561 aacaaacata ttcatacgcc gcgtatcccg caaagaacgt ggcgttaaag cagattccgt
    10621 attgatagta accatttttt cctgccagca gagagtaaga catagtgaaa aaatacgtga
    10681 acaactcaca caggtgtcag gtcggaaaca gcataaatat ggattaaatt gctgcgacat
    10741 gtcgttatgt gatggatatt ccaattttca aattaagttg aattattgag attattatta
    10801 accacctaat tttacagcag ataaaattca taaagttcat taattgataa ttgatatgga
    10861 ttatttcata accatgatat gaattatgat gacctacagg tataaaaaac cctgccatga
    10921 ggcagggtca taaaagtaag aggaatgaat taacgagcca gccaaccgcc atctacggca
    10981 atggtataac cattcacata atctgaagcg ctggaggcaa ggaacactat cggccccatc
    11041 aggtcactcg gcagtcccca acgaccagct ggaatgcggt cgagaatttc cgcgctacgt
    11101 tgttcatctg cccgtagttg ttgagtattg ttggtcgcca tgtaacccgg agctatcgca
    11161 ttaacattaa tgttgtgttt agcccattcg ttcgccatca atcgcgtcac gcccatcacg
    11221 ccgctttttg atgcggtata agaaggcaca cggatcccgc cctggaagga gagcattgac
    11281 gtgatattga taatcttgcc gccattgcgt tgcgcgataa agtgtttcgc cgctgcctga
    11341 gacatgaaga atacgctctt gatattcagg ttcatgacat cgtcccagtc tttttcgctg
    11401 aactcgagag catcttcgcg gcgaatcaat ccggcgttat tcaccaggat atcaatatga
    11461 ccaaactcag ctaccgcgcg atccagcagt gctggaatgc catcaatctt tcgcagatcg
    11521 gcggtcaggc ttaaaaaacg acgccccagc gccgtgacct gcttgatggt ttcagtcggt
    11581 tcaacgatgt taatgccaac aatgtcacag cccgcttgcg ccagccccaa cgccatcccc
    11641 tggcccagtc cagtatcaca accagtgacg accgcaactt taccttcgag agaaaatgca
    11701 cttaaaatca taacaatacc ttattcgttt atgccgacaa ctagcgcaaa tctttaacgg
    11761 ccactatgcc gacaactagc gcaaatcttt aacggccaca tggtccatat catcaaagac
    11821 ctggttttca ccaaccatgc cccagataaa ggtataagct ttggttccga cgccggaatg
    11881 gatcgaccag ctgggggaga tcaccgcctg ctcgttatgc atcacaatgt gacgcgtttc
    11941 ttgcggctgc cccatcatgt ggaaaacgca ggcgtcatca tccatattga aatagaaata
    12001 aacttccatc cggcgctcgt gagtgtgaca cggcatggtg ttccacaagt tacctggagc
    12061 cagctccgtc agccccatac tcaattggca ggtttccagc acatccggga caaaatattt
    12121 gttaattgtg cgacggttac tggtgaggtt atcgcctaac gtgactggag atacttcgtc
    12181 cggtgtgact tttttggtgg gatacgtcgt atgcgcgggt gcgcaattgt aataaaactt
    12241 tgccggagtg gcggtatcaa tactggcaaa gacaacttct tttgcacctt taccaacata
    12301 cagggcgtcg cggtgaccga tttcatagca ttggccatcg acagtaatcg taccggcacc
    12361 gccaatattg ataacaccta actcgcgacg ttcaaggaaa tagcttacgc cgagttgttt
    12421 accaacttcc ccgccaacgg aaaccgtttt agttatcggc ataatgccgc caacaataat
    12481 tcggtcaatg tggctgtaaa ccatggtgta ctcatcggcg acaaatacct tttcaaccaa
    12541 aaattcattg cgcagccctt gggtatccag cgtttttgcg tgcgcactgt ggatgctctg
    12601 tcttacgtcc acatcaacct ccgataatca gtgaagcgtg aaaatagaaa acgatagtga
    12661 aacactgttt cgttttctct ataaagggat gatatgccca tgggctaggg ttttcaatta
    12721 aaaataaaac aatgtttcat ttttaagtta aggattaaaa aaagtgcagt gtcgatcacg
    12781 aatatgtcat gagaatggaa aaatgaggga gcccagaaaa tgtatgtcga cttgccagcc
    12841 atcattattc aacagattaa tgacggcaga tatttatcgg taaggaggaa atgcagagct
    12901 actcgtcacg ttcaatggtc aatgccacgc cctggccccc gccgatacaa agcgttgcca
    12961 gccctttgcg ggcattacgt ttcaccattt catgaaccag agaaaccagg attcggcaac
    13021 cggaagcgcc tatcgggtga ccgagtgcga tcgcgccacc attgacattg acccgacgct
    13081 catcccactc aagcatcttg ccaaccgaaa gcgcctgtgc agcaaacgct tcattagcct
    13141 cgataagatc gacatcagcc aactgccagc ctacacgctc caggcaacgg cgggtcgcat
    13201 acaccggcgc aattcccatc aatgccggat ctacaccaac gctggcaaat gcgcggatgc
    13261 gggccagcac gggtaaattc aacgctcgtg ctttggcttc gctcatcatc attaccgcag
    13321 ctgcgccatc gtttatggat gatgcattcc ccgcggtcac agaaccgaga ctatcaaatg
    13381 aaggatttaa acgggctaaa ccttctgcac tggcgtcagt gcgtggctgt tcatcggtat
    13441 caacaaccaa cgtctgaccg ttactttgga tcattaccgg gacgatctca tctttaaatc
    13501 gtccggcgtc aatcgccgct cgcgcttttt gttgcgagct aagtgcgtaa gcatcctgca
    13561 actgacggct gatgccatat tcgcgagcca gattttcggc ggtgacacca atatgataat
    13621 cattgaaggc atcccacaac ccatcatgca ccagactgtc aaccaactgg ctattgccaa
    13681 gctgtgcacc ggtgcggcta tcagtcagaa catgtggtgc gcggctcatg ttttcctggc
    13741 caccggcgat gacaatatca gcctcgccac actgtatcgc ctgagtagcc agatgcagtg
    13801 ctttaagtcc ggaaccgcaa acgtcattaa tcgtgattgc agaaacgcta ttaggaagac
    13861 cacctttaat agccgattgc cttgccggat tctgccctgc ccctgcagtc aacacctgac
    13921 caagaattac ttcatccacc gcatatgcag gaacgccggt acgttctatt aacgctttca
    13981 cgaccagact accaagttcc acggcggaat gacccgctaa cgcaccacga aagcagccga
    14041 taggtgtccg taacgccccg acaatcacaa cgtctttcat cacaacctcg caccaattaa
    14101 cagtgcaata gtaaatgatt gttaattaca gttatcttaa ttgtttaaaa aaagtgattt
    14161 ttatcacaaa ggaaatatgc ctgagcagta gtcagagtca taactggcac gtaaggtttg
    14221 caaccactaa cccaccaata gatgggtaga taaggcgtta atctcccata cttaacctgg
    14281 tttatggtaa attgccctcc attttgttta atttgtagat gatacgttca gataatgtct
    14341 aatatttggt ctaaagaaga aactctgtgg agtttcgcgc tctacggcac agccgttggt
    14401 gcaggcacgc tcttccttcc tattcagtta ggttcggcag gggctgtggt cctgtttatt
    14461 actgctctgg tcgcctggcc tttaacatat tggccacata aagccttatg ccagttcatc
    14521 ctctcatcaa aaacatcagc atgtgaaggg ataacgggcg cggtaacaca ctactatggc
    14581 aagaagattg gtaatctgat taccacgctg tacttcatcg ccttttttgt cgtcgtgttg
    14641 atatatgcag tggcaattac caactcactt acggaacagc tggcaaagca tatggttatt
    14701 gatcttcgca tccgtatgtt ggtgagtctg ggtgttgtat taattctgaa tctcattttt
    14761 ctgatgggac gtcatgccac tattcgggta atgggatttt tggtattccc attgattgcc
    14821 tatttcttat ttctttccat ttaccttgtc ggtagttggc aacctgatct attaacgacc
    14881 caggtagagt tcaatcagaa tacccttcac cagatatgga tatcgattcc cgtgatggtt
    14941 ttcgccttta gccatacgcc cattatttct acgtttgcca tagacagacg tgaaaaatat
    15001 ggcgaacacg ctatggataa atgcaaaaaa attatgaaag tcgcttatct catcatctgc
    15061 ataagtgtac tgttctttgt ctttagctgc ctgctttcta ttccaccttc gtatattgaa
    15121 gcggctaaag aagaaggggt caccatttta tcggcgcttt ctatgctgcc gaacgcccca
    15181 gcatggttgt caatttccgg gattattgtc gcagtagttg cgatgtcgaa atcattcctg
    15241 ggtacgtact ttggcgttat tgaaggtgcc acggaggtcg tcaaaacaac actacagcag
    15301 gttggtgtaa agaaaagtcg tgcatttaac cgcgcactat caattatgtt ggtatcgctg
    15361 attaccttca ttgtttgttg cattaacccg aacgcgattt cgatgattta tgcgatcagc
    15421 ggcccgctca ttgccatgat acttttcatc atgcctacgt tgtcaacgta tctgatcccg
    15481 gcgcttaaac cctggcgctc catcggaaat ctgattacgc tgatcgtggg tatcctgtgc
    15541 gtatcggtaa tgttctttag ctaatatccg ttgtggatgg ttcaaaattg ctacaatttt
    15601 gaaccattcc atttttcata atattcattt gttattattc cgccctatca cttctctcgt
    15661 catatcctag aattatcagt tagttaaaca cacaaagaga tctgaacatc ttaaataaga
    15721 ctatttaaga tgcataactt agattcgcaa gatatcttct ggtcattcaa aaacaatttg
    15781 cgatatagat attctcatac catgagatag ttatctggac cacgttcgac catgcaagat
    15841 taaatcagtg aaacataatc atatttaata acgagaaatg catttttaaa tgtcttaatc
    15901 agaattatag caaatacaga ttaatcgcaa tttttcccag caaaattaca aaatcaagat
    15961 gtattgacat catcaatcac caatgaaatt atgcaattat atacggatag ggaggttctt
    16021 aacatggggc tttgtagtcg ttataaaagt cttacatgca atagttgctc aatgcattgc
    16081 caaataatgc cagaagagtc accgcgtttg cagtattgcg caaactcgtg tttttgtatg
    16141 tggcccgaag aaagttcata ttttaatcgt ggtgtggtgg aaggtatctt aacaaaaaac
    16201 cacaatgcga gattaagcgg atatattttt gtggattttt cagtaagttt tttacgtcta
    16261 ttccttgaaa aagactggat tgactatctt gcaagtactg acatggggat agttttagtc
    16321 agcgaccgca atatgcaatc gttagctaac tactggcgaa aacacaacac cgcaatatct
    16381 gctgtcatat ataatgatga tggtcttgat gttgccaatg aaaagatcag acaactattt
    16441 attggtcgtt atttatcatt taccagaggt aacacattaa ctcagatgga attcaccata
    16501 atggggtata tggtttctgg ttataatcca tatcagattg ctgaagtgct ggatatggat
    16561 atccgtagca tctacgcgta caagcaacga atcgaaaaga gaatgggtgg taaaataaac
    16621 gaattattta ttcgttcaca ttcggtccaa cattgatact caacgaccag ccagaatcat
    16681 actctgttca tacgggcatc tattgaaatg cccgtatttt ataaataagc agtaacccca
    16741 agagtaaaca acatagtatt taaaaccatc acacgaaaaa ttcagaagca ttacgaaata
    16801 tgaatttaca aacaatagtg gcataaatgt taaccatgtt aatttacgta aagttttacg
    16861 ttgcaacatt aaagcctcat ttcaatcatc atgataaata taaaattaat atatatttat
    16921 gccgtaaata ccaatatact tagcaaacta tgtgatctcc atttcgattg atttagtgtt
    16981 tattgacgta tgtactggat tattaacgat aatatcgagt tctggcctga gcaccgaaaa
    17041 ttaatatcgg tacataacgc cgatcttaac gtcgttctga caacgccagc cagtcgatgt
    17101 ttatcacttc tacttgaagc ttttcctgat gtggttgcac aacaagattt tttcaccaga
    17161 gtctgggaag aagaaggtat gcgtgtgcct actaacacgt tatatcagaa catatccatt
    17221 atcagacgcg gatttcgcgc tgttggtgat actacccact cgctaattgc aaccgtgccg
    17281 agagaggatt caa
//
LOCUS       DAJVQG010000087        16963 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJVQG010000087 DAJVQG010000000
VERSION     DAJVQG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16963
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.087"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(155..565)
                     /locus_tag="OQM83_002546"
     CDS             complement(155..565)
                     /locus_tag="OQM83_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HCS1520785.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFR
                     WITNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
     gene            complement(623..856)
                     /locus_tag="OQM83_002547"
     CDS             complement(623..856)
                     /locus_tag="OQM83_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HCS1520786.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            1245..1790
                     /locus_tag="OQM83_002548"
     CDS             1245..1790
                     /locus_tag="OQM83_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCS1520787.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1765..3690
                     /locus_tag="OQM83_002549"
     CDS             1765..3690
                     /locus_tag="OQM83_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCS1520788.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQRDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3687..3893
                     /locus_tag="OQM83_002550"
     CDS             3687..3893
                     /locus_tag="OQM83_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HCS1520789.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3890..5491
                     /locus_tag="OQM83_002551"
     CDS             3890..5491
                     /locus_tag="OQM83_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCS1520790.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5472..6791
                     /locus_tag="OQM83_002552"
     CDS             5472..6791
                     /locus_tag="OQM83_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCS1520791.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6801..7133
                     /locus_tag="OQM83_002553"
     CDS             6801..7133
                     /locus_tag="OQM83_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCS1520792.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            7189..8214
                     /locus_tag="OQM83_002554"
     CDS             7189..8214
                     /locus_tag="OQM83_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCS1520793.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            8256..8654
                     /locus_tag="OQM83_002555"
     CDS             8256..8654
                     /locus_tag="OQM83_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCS1520794.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8666..9019
                     /locus_tag="OQM83_002556"
     CDS             8666..9019
                     /locus_tag="OQM83_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HCS1520795.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            9031..9609
                     /locus_tag="OQM83_002557"
     CDS             9031..9609
                     /locus_tag="OQM83_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1520796.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9606..10001
                     /locus_tag="OQM83_002558"
     CDS             9606..10001
                     /locus_tag="OQM83_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1520797.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            10009..10749
                     /locus_tag="OQM83_002559"
     CDS             10009..10749
                     /locus_tag="OQM83_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1520798.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            10765..11187
                     /locus_tag="OQM83_002560"
     CDS             10765..11187
                     /locus_tag="OQM83_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCS1520799.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            11169..11603
                     /locus_tag="OQM83_002561"
     CDS             11169..11603
                     /locus_tag="OQM83_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCS1520800.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11596..14157
                     /locus_tag="OQM83_002562"
     CDS             11596..14157
                     /locus_tag="OQM83_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1520801.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSYASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAVKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            14154..14483
                     /locus_tag="OQM83_002563"
     CDS             14154..14483
                     /locus_tag="OQM83_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1520802.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14483..15181
                     /locus_tag="OQM83_002564"
     CDS             14483..15181
                     /locus_tag="OQM83_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCS1520803.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            15187..15930
                     /locus_tag="OQM83_002565"
     CDS             15187..15930
                     /locus_tag="OQM83_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1520804.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            15867..16499
                     /locus_tag="OQM83_002566"
     CDS             15867..16499
                     /locus_tag="OQM83_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCS1520805.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRAMATQLPAFRQKLSD
                     GWYQVRIAGQDVSTSGLTAQLHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVMPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16560..>16963
                     /locus_tag="OQM83_002567"
     CDS             16560..>16963
                     /locus_tag="OQM83_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCS1520806.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEV"
BASE COUNT         3887 a         4211 c         5239 g         3626 t
ORIGIN      
        1 cccgtattaa cagagaccgt agcccgacgg ggaactcctt ctgcgcgagt gtgcgggaat
       61 aatcaaaaac gatgcacacc ggggttaccg ggtacacata tttcatcatg ccagcgagtc
      121 cggttctggc acggaagaaa ccggacgtta tgatttagtg cggaaatatt tgtgtagtgt
      181 tctgtatgtt ctcagtaaag agtaatgaat tatcaaaggt atagtaatac cttttgtttt
      241 cgtggatatt tgtaatccat ctgaaaaccc ctgctgtagc aagatttttc ctgtattcgt
      301 aaaatgataa ctctcctgat ttgaatcctt ttaaggtggc ttctataagg catttatttt
      361 ttgaaaatct tacatttaca accttaccct gtccttttat taaaaccgta ttatcgtttt
      421 caagaacaag atgaatattc tctgtggcta aatagtaaat gtaatgtgag acattgtgac
      481 gttttagttc agaataaaac cagtgatagt ttaaattatt tcgcacttta tcgaatattt
      541 gtttaaaaat ggcaacctga gccattgtag taccttccat gtgatatgag ggggcgtagt
      601 ctgcacgatt atctaaattg cttcaatctg gtctaacctg ttttctgagc aattcagtaa
      661 tgtcactctt ttctttgttt gcttcaggcg aaactctttt ttctgagcac agtctccggc
      721 ggcaggcttc aatgacccag gctgagaaat tcccggaccc tttttgaaca agagcgatgt
      781 taatttgttc aatcatttgg ttaggaaagc ggatgttgcg ggttgttgtt ctgcgggttc
      841 tgttcttcgt tgacatgagg ttgccctgta ttcagtgtcg ctgatttgta ttgtctgaag
      901 ttgtttttac gttaagttga tgcagatcaa ttaatacgat acctgcgtca taattgatta
      961 tttgacgtgg tttgatggcg tagatgcacg ttgtgacatg tagatgataa ttattatcat
     1021 tttgcgggtc ctttccggcg atccgacagg ttacggggcg gaaggcgcgc gggttttcgc
     1081 tatttatgac aattttccgg tttaaggcgt ttccgttctt cttcgccgta acctaatgtt
     1141 tttatttaaa acaccccctg aaaagaaagg aaacgacagg tgctgaaaac gggctttttg
     1201 gcctctgtcg tttcctttct ctgtttttgt ccgtggaatg aacaatggaa gtcaacaaaa
     1261 agcagctggc tgacattttc ggtgcgagta tccgtaccat tcagaactgg caggaacagg
     1321 gaatgcccgt tctgcgaggc ggtggcaagg gtaatgaggt gctttatgac tctgccgccg
     1381 ttataagatg gtatgccgaa agggatgctg aaattgagaa cgaaaagctg cgccgggaag
     1441 ttgaagaact gcggcaggcc agcgagacag atctccagcc agggactatt gagtacgaac
     1501 gccatcgact tacgcgtgcg caggccgacg cacaggagct gaaaaatgcc agagactccg
     1561 ctgaagtggt ggaaaccgca ttctgtactt tcgtgctgtc gcggatcgca ggtgaaattg
     1621 ccagtattct cgacgggatc cccctgtcgg tgcagcggcg ttttccggaa ctggaaaacc
     1681 gacatgttga tttcctgaaa cgggatatca tcaaagccat gaacaaagca gccgcgctgg
     1741 atgaactgat accggggttg ctgagtgaat atatcgaaca gtcaggttaa caggctgcgg
     1801 cattttgtcc gcgccgggct tcgctcactg ttcaggccgg agccacagac cgccgttgaa
     1861 tgggcggatg ctaattacta tctcccgaaa gaatccgcat accaggaagg gcgctgggaa
     1921 acactgccct ttcagcgggc catcatgaat gcgatgggca gcgactacat ccgtgaggtg
     1981 aatgtggtga agtctgcccg tgtcggttat tccaaaatgc tgctgggtgt ttatgcctac
     2041 tttatagagc ataagcagcg caacacactt atctggttgc cgacggatgg tgatgccgag
     2101 aactttatga aaacccacgt tgagccgacc atccgcgata ttccgtcgct gctggcgctg
     2161 gctccgtggt atggcaaaaa gcaccgggat aacacgctca ctatgaagcg tttttccaat
     2221 ggtcgtggct tctggtgcct gggcggtaaa gcggcaaaaa actaccgtga aaagtcggtg
     2281 gatgtggcgg gttatgatga acttgctgcc tttgatgagg atattgaaca ggaaggctct
     2341 ccgacgttcc ttggcgacaa acgtattgaa ggctcggtct ggccaaagtc catccgtggc
     2401 tccacgccca aagtgagagg cacctgccag attgagcgtg cagccagtga atccccgcat
     2461 tttatgcgtt ttcatgttgc ctgcccgcac tgcggggagg agcagtatct taaatttggc
     2521 gacaaagaga cgccgtttgg cctcaaatgg acgccggatg acccctccag cgtgttttat
     2581 ctctgcgagc ataatgcctg cgtcatccgc cagcaggagc tggactttac tgatgcccgt
     2641 tatatctgcg aaaagaccgg gatctggacc cgtgatggca ttctctggtt ttcgtcatcc
     2701 ggtgaagaga ttgagccacc tgacagtgtg acctttcaca tctggacagc gtacagcccg
     2761 ttcaccacct gggtgcagat tgtcaaagac tggatgaaaa cgaaagggga tacgggaaaa
     2821 cgtaaaacct tcgtaaacac cacgctcggt gagacgtggg aggcgaaaat tggcgaacgt
     2881 ccggatgctg aagtgatggc agagcggaaa gagcattatt cagcgcccgt tcctgaccgt
     2941 gtggcttacc tgaccgccgg tatcgactcc cagcgggatc gctacgaaat gcgcgtatgg
     3001 ggatgggggc cgggtgagga aagctggctg attgatcggc agattattat gggccgccac
     3061 gacgatgaac agacgctgct gcgtgtggat gaggccatca ataaaaccta tacccgccgg
     3121 aatggtgcag aaatgtcgat atcccgtatc tgctgggata ttggcgggat tgacccgacc
     3181 attgtgtatg aacgctcgaa aaagcatggg ctgttccggg tgatccccat taaaggggca
     3241 tccgtctacg gtaagccggt ggccagcatg cctcgtaagc gaaacaaaaa cggggtttac
     3301 cttaccgaaa tcggtacgga taccgcgaaa gagcagattt ataaccgctt cacactgacg
     3361 ccggaagggg atgaaccgct tcccggtgcc gttcacttcc cgaataaccc ggatattttt
     3421 gatctgaccg aagcgcagca gctgactgct gaagagcagg tcgaaaaatg ggtggatggc
     3481 aggaaaaaaa tactgtggga cagcaaaaag cgacgcaatg aggcgctcga ctgcttcgtt
     3541 tatgcgctgg cggcgctgcg catcagtatt tcccgctggc agctggatct cagtgcactg
     3601 ctggcgagcc tgcaggaaga ggatggtgca gcaaccaaca agaaaacact ggcagattac
     3661 gcccgtgcct tatccggaga ggatgaatga cgcgacagga agaacttgcc gctgcccgtg
     3721 cggcactgca tgacctgatg acaggtaaac gggtggcaac ggtacagaaa gacggacggc
     3781 gagtggagtt tacggccact tccgtgtctg acctgaaaaa atacattgcg gagctggagg
     3841 tgcagaccgg catgacacag cgacgcaggg gacctgcagg attttatgta tgaaaacgcc
     3901 caccattccc acccttctgg gaccggacgg catgacatcg ctgcgtgaat atgccggtta
     3961 tcacggcggt ggcagcggat ttggtgggca gttgcgggcg tggaacccac cgggtgaaag
     4021 tgtggatgca gccctgctgc ccaactttac ccgtggcaat gcccgcgcag acgatctggt
     4081 acgcaataac ggctatgctg ccaacgccat ccagctgcat caggatcata tcgtcgggtc
     4141 ttttttccgg ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg aggaagacgc
     4201 ccgtgccttt tcccgcgagg ttgaagcggc atggaaagag tttgccgagg atgactgctg
     4261 ctgcattgac gttgagcgaa aacgcacgtt taccatgatg attcgggaag gtgtggccat
     4321 gcacgccttt aacggtgaac tgttcgttca ggccacctgg gataccagtt cgtcgcggct
     4381 tttccggaca cagttccgga tggtcagccc gaagcgcatc agcaacccga acaataccgg
     4441 cgacagccgg aactgccgtg ccggtgtgca gattaatgac agcggtgcgg cgctgggata
     4501 ttacgtcagc gaggacggct atcctggctg gatgccgcag aaatggacat ggataccccg
     4561 tgagttaccc ggcgggcgcg cctcgttcat tcacgttttt gaacccgtgg aggacgggca
     4621 gacccgcggt gcaaatgtgt tttacagcgt aatggagcag atgaagatgc tcgacacgct
     4681 gcagaacacg cagctgcaga gcgccattgt gaaggcgatg tatgccgcca ccattgaaag
     4741 tgagctggat acgcagtcag cgatggattt tattctgggc gcgaacagta aggagcagcg
     4801 ggaaaggctg acgggctgga ttggtgaaat tgccgcgtat tacgccgcag caccggtccg
     4861 tctgggaggc gcaaaagtgc cgcacctgat gccgggggac tcactgaacc tgcagacggc
     4921 tcaggacacg gataacggct actccgtgtt tgagcagtca ctgttgcggt atatcgctgc
     4981 cgggctgggt gtctcgtatg agcaactttc ccggaattac gcccagatga gctactccac
     5041 ggcacgggcc agtgcgaacg agtcgtgggc gtactttatg gggcggcgaa aattcgtcgc
     5101 atcccgtcag gcgagccaga tgtttctgtg ctggctggaa gaggccatcg ttcgccgcgt
     5161 ggtgacgtta ccttcaaaag cgcgcttcag ctttcaggaa gcccgcagtg cctgggggaa
     5221 ctgcgactgg ataggctccg gtcgtatggc catcgatggt ctgaaagaag ttcaggaagc
     5281 ggtgatgctg atagaagccg gactgagcac ctacgagaaa gagtgcgcga aacgcggtga
     5341 cgactatcag gaaatttttg cccagcaggt ccgtgaaacg atggagcgcc gcgcagctgg
     5401 tcttaaaccg cccgcctggg cggctgcggc atttgaatcc gggctgcgac aatcaacaga
     5461 ggaggagaag agtgacagca gagctgcgta atctcccgca tattgccagc atggctttta
     5521 atgagccgct gatgcttgaa cccgcctatg cgcgggtttt cttttgtgcg cttgcaggcc
     5581 agcttgggat cagctgcctg acggatgcag tatccggcga cagcctgact gccggagagg
     5641 cacccgcggc gctggcgtta tccggtgatg atgacggacc acgacaggcc cgcagttatc
     5701 aggtaatgaa cggcatcgcc gtgctgccgg tgtccggcac gctggtcagc cggacgcggg
     5761 cgctgcagcc gtactcgggg atgaccggtt acaacggcat tatcgcccgt ctgcaacagg
     5821 ctgccagcga cccgatggtg gacggcattc tgctcgatat ggacacaccg ggcggaatgg
     5881 tggcgggagc atttgactgc gctgacatca tcgcccgtgt gcgtgacata aagccggtat
     5941 gggcgctggc caatgacatg aactgcagcg caggtcagct gcttgccagt gccgcctccc
     6001 ggcgtctggt cacgcagacc gcccggacag gctccatcgg cgtcatgatg gctcacagta
     6061 attacggcgc tgcactggag aaacagggcg tggaaatcac gctgatttac agcggcagcc
     6121 ataaggtgga tggcaacccc tacagccatc taccggatga tgtccgggaa acattgcagt
     6181 cccggatgga tgcaacccgc cggatgtttg cacagaaggt gtcggcatat accggcctgt
     6241 ccgtgcaggc tgtgctggat accgaggctg cagtgtacag cggtcaggag gccattgatg
     6301 ccggactggc tgatgaactt gttaacagta ccgatgcgat caccgtcatg cgtgatgcac
     6361 tggatgcacg taaatcccgt ctctcaggag ggcgaatgac caaagagact caatcaacaa
     6421 ctgtttcagc cactgcttcg caggctgacg ttactggcgt ggtgcaagcg acggagggcg
     6481 agaacgccag cgcggcgcag ccggacgtga acgcgcagat caccgcagcg gttgcggcag
     6541 aaaacagtcg cattatgggg atcctcaact gtgaggaggc tcacggacgc gaagaacagg
     6601 cacgcgtgct ggccgaaacc cccggtatga ccgtggaaac ggcccgccgt attctggccg
     6661 cagcaccaca gagtgcacag acgcgcagtg acactgcgct ggatcgtctg atgcaggggg
     6721 caccggcacc gctggctgca ggtaacccgg catctgatgc cgttaacgat ttgctgaaca
     6781 caccagtgta agggatgttt atgacgagca aagaaacctt tacccattac cagccgctgg
     6841 gcaatagtga cccggctcat accgcaaccg cgcccggcgg attgagtgcg aaagcgcctg
     6901 caatgacccc gctgatgctg gacacctcca cccgtaagct ggttgcgtgg gatggcacca
     6961 ccgacggtgc tgccgttggc attcttgcgg ttgctgctga ccagaccagc accacactga
     7021 cgttctacaa gtccggcacg ttccgttatg aggatgtgct ctggccggag gctgccagcg
     7081 acgagacgaa aaaacggacc gtgtttgccg gaacggcaat cagcatcgtt taactttacc
     7141 cttcatcact aaaggccgcc tgtgtggctt tttttacggg atttttttat gtcgatgtac
     7201 acaaccgccc agctgctggc ggcaaatgag cagaaattta agtttgatcc gctgtttctg
     7261 cgtctctttt tccgtgagag ctatcccttc actacggaga aagtctatct ctcacaaatt
     7321 ccgggactgg taaacatggc gctgtacgtt tcgccgattg tttccggtga ggttatccgt
     7381 tcccgtggcg gctccacctc tgaatttacg ccgggatatg tcaaacccaa gcatgaggtg
     7441 aatccgcaga tgaccctgcg tcgcctgccg gatgaagatc cgcagaatct ggcggacccg
     7501 gcttaccgcc gccgtcgcat catcatgcag aacatgcgtg acgaagagct ggccattgct
     7561 caggtcgaag agatgcaggc agtttctgcc gtgctcaagg gcaaatacac catgaccggt
     7621 gaagccttcg atccggttga ggtggatatg ggccgcagtg cggcgaacaa catcacacag
     7681 tccggcgtca cggagtggag caagcgtgac aagtccacgt atgacccgac cgacgatatc
     7741 gaagcctacg cgctgaacgc cagcggtgtg gtgaatatca tcgtgtttga cccgaaaggc
     7801 tgggcgctgt tccgttcctt caaagccgtc aaggagaagc tggatacccg tcgcggctct
     7861 cattccgagc tggagacagc ggtaaaagac ctgggcaaag cggtgtccta taagggaatg
     7921 tatggcgatg tggccatcgt cgtgtattcc ggacagtacg tggaaaacgg cgtcaaaaag
     7981 aacttcctgc cggacaacac gatggtgctg gggaacactc aggctcgcgg tctgcgcacc
     8041 tatggctgca ttcaggatgc ggacgcacag cgcgaaggca ttaacgcttc tgcccgttac
     8101 ccgaaaaact gggtgaccac cggcgatccg gcgcgtgagt tcaccatgat tcagtcagca
     8161 ccgctgatgc tgctggctga tcctgatgag ttcgtgtccg ttcaactggc gtaatcatgg
     8221 cccttcgggg ccattttctc tctgtggagg agttcatgac gaaagatgaa ctgattgccc
     8281 gtcttcagga gctgggtgag caattgaacc gcgatgtcag tttgacggga acgaaagaag
     8341 aactggcgct tcgtgtggca gagctggaag aagagcttga tgacacggat gaaactgccg
     8401 gtcaggacac ccctctcagc cgggaaaatg tgctgaccgg gcatgaaaat gaggtggtat
     8461 cagcgcagcc ggataccgtg attcaggata cggctgaact ggtcacggtc gtggcactgg
     8521 tgacgctgca tactgatgca cttcacgcca cgcgggataa acctgtggca tttgtgctgc
     8581 cgggaacggc gtttcgtgtc tctgccggtg tggcagccga aatgacagag cgcggcctgg
     8641 ccagaatgca ataacgggag gcgctgtggc tgatttcgat aacctgttcg atgctgccat
     8701 tgcccgcgcc gatgaaacga tacgcgggta catgggaacg tcagccacca tgacatccgg
     8761 tgagcagtca ggtgcggtga tacgtggtgt ttttgatgac cctgaaaata tcagctatgc
     8821 cggacagggc gtgcgcgttg aaggctccag cccgtccctg tttgtccgga ctgatgaggt
     8881 gcggcagctg cggcgtggag acacgctgac catcggtgag gaaaatttct gggtagatcg
     8941 ggtttcgccg gatgatggcg gaagttgtca tctctggctt ggacggggcg taccgcctgc
     9001 cgttaaccgt cgccgctgaa agggggatgt atggccataa aaggtcttga gcaggccgtt
     9061 gaaaacctca gccgtatcag caaaacggcg gtgcccggtg ccgccgcaat ggccattaac
     9121 cgcgttgctt catccgcgat atcgcagtct gcgtcacagg ttgcccgtga gacaaaggta
     9181 cgccggaaac tggtaaagga aagggccagg ctgaaaaggg ccacggtcaa aaatccgcag
     9241 gccagaatca aagttaaccg gggggatttg cccgtaatca agctgggtaa tgcgcgggtt
     9301 gtcctgtccc gccgcaggcg tcataaaaag gggcagcgtt catccctgaa aggtggcggc
     9361 agcgtgcttg tggtgggtaa ccgtcgtatt cccggcgcgt ttattcagca actgaaaaac
     9421 ggccggtggc atgttatgca gcgtgtggcc gggaaaaacc gttaccccat tgatgtggtg
     9481 aaaatcccga tggcggtgcc gctgaccacg gcgtttaaac agaatattga acggatacgg
     9541 cgtgaacgtc ttccgaaaga gctgggctat gcgctgcagc atcaactgag aatggtaata
     9601 aagcgatgaa acatactgaa ctccgtgcag ccgtactgga tgcactggag aagcatgaca
     9661 ccggggcgac gctttttgat ggtcgccccg ctgtttttga tgaggaagat tttccggcaa
     9721 ttgccgttta tctcaccggc gctgaataca cgggcgaaga gctggacagc gatacctggc
     9781 aggcggagct gcatattgaa gttttcctgc ctgctcaggt gccggattca gagctggatt
     9841 cgtggatgga gtcccggatt tatccggtga tgagcgatat cccggcactg tcagatttga
     9901 tcaccagtat ggtggccagc ggctatgact accggcgcga cgatgatgcg ggcctgtgga
     9961 gttcagccga tctgacttat gtcattatct atgaaatgtg aggacgctat gcctgtacca
    10021 aatcctacaa tgccggtgaa aggtgccggg accaccctgt gggtttataa ggggagcggt
    10081 gacccttatg cgaacccgct ttcagacgtt gactggtcgc gtctggcaaa agttaaagac
    10141 ctgacgcccg gcgaactgac cgctgagtcc tatgacgaca gctatctcga tgatgaagat
    10201 gcagactgga ctgcgaccgg gcaggggcag aaatctgccg gagataccag cttcacgctg
    10261 gcgtggatgc ccggagagca ggggcagcag gcgctgctgg cgtggtttaa tgaaggtgat
    10321 acccgtgcct ataaaatccg cttcccgaac ggcacggtcg atgtgttccg tggctgggtc
    10381 agcagtatcg gtaaggcggt gacggcgaag gaggtgatta cccgcacggt gaaggtcacc
    10441 aatgtgggac gtccgtcgat ggcagaagat cgcagcacgg tgacggcgac gaccggcatg
    10501 acggtgacac ccgccagtgc ttccgtagtg aaagggcaga gcaccacgct gaccgtggca
    10561 ttccagccgg atggcgcaac cgacaagagc ttccgtgcgg tgtctgcgga taaaacaaaa
    10621 gccaccgtgt cggtcagtgg tatgaccatc accgtgaaag gtgttgctgc aggcaaggtc
    10681 aacattccgg tcgtatctgg taatggtgag tttgctgcgg ttgcagaaat caacgtcacc
    10741 gccagttaat ccggagagtc agcgatgttc ctgaaaaccg aatcatttga acataacggt
    10801 gtgaccgtca cgctttctga actgtcagcc ctgcagcgta ttgagcatct cgacctgatg
    10861 aaacggcagg cagaacaggc ggagtcagac agcaaccgga agtttactgt ggaagacgcc
    10921 atcagaaccg gcgcttttgt ggttgcgatg tccctgtggc ataaccatcc gaagaagacg
    10981 cagatgccgt ccatgaatga agccgttaaa cagattgagc aggaagtgct taccacctgg
    11041 cccacagagg caatttctca tgctgaaaac gtggtgtacc ggctgtccgg tatgtatgag
    11101 tttgtggtga atgatgctcc tgaacaggca gaggacgccg ggcctgcaga gcctgtttct
    11161 gcgggaaagt gttcgacggt gagctgagtt ttgccctgaa actggcgcgt gagatggggc
    11221 gacccgactg gcgcgccatg cttgccggga tgtcatccac ggagtatgcc gactggcacc
    11281 gcttttacag tacccattat tttcatgatg ttctgctgga tatgcacttt tccgggctga
    11341 cgtacaccgt gctcagcctg tttttcagcg atccggagat gcatccgctg gatttcagtc
    11401 tgctgaaccg gcgcgaggct gacgaagagc ctgaagatga tgtgctgatg cagaaagcgg
    11461 cagggcttgc cggaggtgtc cgctttggcc cggacgggaa tgaagttatc cccgcttccc
    11521 cggatgtggc ggacatgacg gaggatgacg taatgctgat gacagtatca gaagggatcg
    11581 caggaggagt ccggtatggc tgaaccggta ggcgatctgg tcgttgattt gagtctggat
    11641 gcggccagat ttgacgagca gatggccaga gtcaggcgtc atttttccgg tacggaaagt
    11701 gatgcgaaaa aaacagcggc agtcgttgaa cagtcgctga gccgacaggc gctggctgca
    11761 cagaaagcgg ggatttccgt cgggcagtat aaagccgcca tgcgtatgct gcctgcacag
    11821 ttcaccgacg tggccacgca gcttgcaggg gggcagaatc cctggctgat cctgctgcaa
    11881 cagggtggtc aggttaagga ctccttcggc gggatgatcc ccatgttcag ggggcttgcc
    11941 ggcgcgatca ccctgccgat ggtgggggcc acctcgctgg cggtggcgac cggtgcgctg
    12001 gcgtatgcct ggtatcaggg caactcaacc ctgtccgatt tcaacaaaac gctggtcctt
    12061 tccggtaatc aggcgggact gacggcagat cgtatgctgg tcctgtccag agccgggcag
    12121 gcggcagggc tgacgtttaa ccagaccagc gagtcactca gcgcactggt taaggcgggg
    12181 gtaagcggtg aggctcagat tgcgtccatc agccagagtg tggcgcgttt ctcctatgca
    12241 tccggcgtgg aggtggacaa ggtcgctgaa gccttcggga agctgaccac agacccgacg
    12301 tcggggctga cggcgatggc acgccagttc cataacgtga cggcggagca gattgcgtat
    12361 gttgctcagt tgcagcgttc cggcgatgaa gccggggcat tgcaggcggc gaacgaggcc
    12421 gcaacgaaag ggtttgatga ccagacccgc cgcctgaaag agaacatggg cacgctggag
    12481 acctgggcag acaggactgc acgtgcattc aaatccatgt gggatgcggt gctggatatt
    12541 ggtcgtcctg ataccgcgca ggagatgctg attaaggcag aggctgcgtt taagaaagca
    12601 gacgacatct ggaatctgcg caaggatgat tatttcgtta acgatgaagc acgggcgcgt
    12661 tactgggatg atcgtgaaaa aaaacgcctg gagcgggatg cagcccaaaa gagggtagat
    12721 caacagcgtc aacaggacaa aaatgcgcag cagcagagcg ataccgaagc gtcacggctg
    12781 aaatataccg aagaggcgca gaaggcttac gaacggctgc agacgccgct ggagaaatat
    12841 accgcccgtc aggaagaact gaacaaggca ctgaaagacg gaaaaatcct gcaggcagat
    12901 tacaacacgc tgatggcggc ggcgaaaaag gattatgaag cgacgctgaa aaagccgaaa
    12961 cagtccggcg tgaaggtgta tgcgggcgat cgtcaggaag acagtgctca tgcggccctg
    13021 ctgacgcttc aggcagaact ccggacgctg gagaagcatg ccggagcgaa tgagaaaatc
    13081 agccagcagc gccgggattt gtggaaggca gaaagtcagt tcgcggtact ggaggaggcg
    13141 gcacaacgtc gccagctgtc cgcacaggag aaatccctgc tggcgcataa agacgagacg
    13201 ctggagtaca aacgccagct ggctgcactt ggtgacaagg ttacgtatca ggagcacctg
    13261 aatgcgctgg cgcagcaggc ggataaattc gcacagcagc aacgggcaaa acgggcagcc
    13321 attgatgcga aaaaccgggg gctgactgac cggcaggcag cgcgggaagc cacggaacag
    13381 cgcctgaagg aacagtatgg cgataatcct ctggcgctga ataacgtcat gtcagagcag
    13441 aaaaagacct gggcggctga agaccagctt cgcgggagct ggatggcagg cctgaagtcc
    13501 ggctggagtg agtgggaaga gagcgccacg gacagtatgt cacaggttaa aagtgcagcc
    13561 acgcagacct ttgatggtat tgcacagaat atggcggcga tgctgaccgg cagtgaacag
    13621 aactggcgca gcttcacccg ctccgtgctg tccatgatga cagaaattct gcttaagcag
    13681 gcaatggtgg ggattgtcgg gagtatcggc agcgccattg gcggggctgt tggtggcggc
    13741 gcatccgcgt caggtggtac agccattcag gccgctgcgg tgaaattcca ttttgcaacc
    13801 gggggattta cgggaaccgg cggcaaatat gagccagcgg ggattgttca ccgtggtgaa
    13861 tttgtcttca cgaaggaggc aaccagccgg attggcgtgg ggaatctcta ccggctgatg
    13921 cgcggctatg cggaaggtgg ttatgtgggc ggtgccggaa gtccggcgca gatgcggcgg
    13981 gctgaaggca ttaattttaa tcagaacaat cacgtggtga ttcagaacga cggtacgaat
    14041 ggtctgccag gtccacagat gatgaaggcc gtgtatgaca tggcccgcaa gggtgcccgt
    14101 gatgaaattc agacacagat gcgtgatggt ggcctgttct ccggaggtgg acgatgaaaa
    14161 ccttccgctg gaaagtgaaa cccggtatgg atgtggcttc ggccccttct gtaagaaagg
    14221 tgcgctttgg tgatggctat tcccagcgag cgcctgccgg gctgaatgcc aacctgaaaa
    14281 cgtacagcgt gacgctttct gtcccccgtg aggaggccat ggtactggag tcgtttctgg
    14341 aagagcacgg gggctggaaa gcctttctgt ggacgccgcc ttatgagtgg cggcagataa
    14401 aggtgacctg cgcaaaatgg tcgtcgcggg tcagtatgct gcgtgttgag ttcagcgcag
    14461 agtttgaaca ggtggtgaac tgatgcagga tatccggcag gaaacactga atgaatgcac
    14521 ccgtgcggag cagtcggcca gcgtggtgct ctgggaaatc gatctgacag aggtcggtgg
    14581 agaacgttat tttttctgta atgagcagaa cgaaaaaggt gagccggtca cctggcaggg
    14641 gcgacagtat cagccgtatc ccattcaggg gagtggtttt gaactgaatg gcaaaggcac
    14701 cagtacgcgc cccacgctga tggtttctaa cctgtacggt atggtcaccg ggatggcgga
    14761 agatctacag agtctggtcg gcggaacggt ggtccggcgt aaggtttacg cccgttttct
    14821 ggatgcggtg aacttcgtca acggaaacag tgacgccgat ccggagcagg aggtgatcag
    14881 ccgctggcgc attgagcagt gcagcgaact gagcgcggtg agtgcctctt ttgtactgtc
    14941 cacgccgacg gaaacagatg gcgctgtttt tccgggacgt atcatgctgg ccaacacctg
    15001 cacctggacc tatcgcggcg atgagtgcgg ttatcacggt ccggcggtcg cggatgaata
    15061 tgaccagcca acgtccgaca tcacgaagga taaatgcagc aaatgcctga gtggctgtaa
    15121 gtttcgcaat aacgtcggca actttggcgg cttcctttcc attaacaaac tttcgcagta
    15181 aatcccatga cagagacaga atcagcgatt ctggcgcacg cccggcgatg tgctccagcg
    15241 gagtcgtgcg gcttcgtggt aagaacgccg gagggggaaa gatatttccc ctgcgtgaat
    15301 atctccggtg agccggaggc gtatttccgt atgtcgccgg aagactggct gcaggcagag
    15361 atgcagggtg agattgtggc actggtccac agccaccccg gtggtctgcc ctggctgagt
    15421 gaggccgacc ggcggctgca ggtgcagagt gatttgccgt ggtggctggt ctgccggggg
    15481 gcgattcaca agttccgctg tgtgccacat cttaccgggc ggcgctttga gcacggggtg
    15541 acggactgtt acacgctgtt ccgggatgct taccatctgg cggggattga gatgccggat
    15601 tttcatcgtg aggatgactg gtggcgtcac ggtcagaatc tctatctgga taatctggag
    15661 gccacagggc tgtatcaggt gccgttgtca gcggcgcagc cgggcgatgt gctgctgtgc
    15721 tgttttggtt catcggtgcc gaatcatgcc gccatttact gtggtgacgg cgagctgctg
    15781 caccatattc ctgaacaact gagcaaacga gagaggtata ccgacaaatg gcagcgacgc
    15841 acacactccc tctggcgtca ccgggcatgg cacgcatctg cctttacggg gatttgcaac
    15901 gatttggccg ccgcatcgac cttcgtgtga aaacgggtgc cgaagccatc cgggcgatgg
    15961 ccacacagct ccctgcgttt cgtcagaagc tgagcgacgg ctggtatcag gtgcgtattg
    16021 ccgggcagga tgttagcacg tccggattaa cggcacagtt acatgaggct ctgcctgacg
    16081 gcgctgtgat tcatattgtt cccagagtcg ccggggccaa gtcaggtggt gtattccaga
    16141 ttgtcctggg agcagccgcc attgccggat cattctttac cgccggagcc acccttgcag
    16201 catggggggc tgccattggt gccggtggta tgaccggcat tctgttttct ctcggtgcca
    16261 gtatggtgct cggcggtgtg gctcagatgc tggcaccgaa agccagaact ccccgcacac
    16321 agacaacgga taacggtaag cagaacacct atttctcctc actggataac atggttgccc
    16381 agggcaatgt tatgcctgtt ctgtatggtg aaatgcgcgt ggggtcacgt gtggtatctc
    16441 aggagatcag cacggcagac gaaggggacg gtggtcaggt tgtggtgatt ggtcgctgat
    16501 gcaaaatgtt ttatgtgaaa tcgcctccgg gcggttttat cgtttatgga gcatgacgaa
    16561 tgggtaaagg cagcagtaag gggcataccc cgcgcgaagc gaaggacaac ctgaagtcca
    16621 cgcagctgct gagtgtgatc gatgccatca gcgaagggcc ggttgaaggt ccggtggatg
    16681 gattaaaaag cgtgctgctg aacagtacgc cggtgctgga cagtgagggg aataccaaca
    16741 tctccggcgt cacggtggtg ttccgggcag gtgagcagga gcagtcaccg ccggagggat
    16801 ttgaatcctc cggctccgag acggtgctgg gtacggaagt gaaatatgac acgccgatca
    16861 cccggaccat cacgtcggca aacattgacc gactgcgttt taccttcggt gtacaggcac
    16921 tggtggaaac cacctcaaag ggagacagga atccgtctga agt
//
LOCUS       DAJVQG010000088        16739 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJVQG010000088 DAJVQG010000000
VERSION     DAJVQG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.088"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..635
                     /locus_tag="OQM83_002568"
     CDS             <1..635
                     /locus_tag="OQM83_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCS1520807.1"
                     /translation="VFIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDTTYCIKYAIL
                     AFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQRKMIIAQLIYYFGI
                     AVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPL
                     LKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIMPSLPFL"
     gene            complement(691..3546)
                     /gene="valS"
                     /locus_tag="OQM83_002569"
     CDS             complement(691..3546)
                     /gene="valS"
                     /locus_tag="OQM83_002569"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCS1520808.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(3546..3989)
                     /gene="holC"
                     /locus_tag="OQM83_002570"
     CDS             complement(3546..3989)
                     /gene="holC"
                     /locus_tag="OQM83_002570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCS1520809.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(4123..5634)
                     /gene="pepA"
                     /locus_tag="OQM83_002571"
     CDS             complement(4123..5634)
                     /gene="pepA"
                     /locus_tag="OQM83_002571"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCS1520810.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            5901..7001
                     /gene="lptF"
                     /locus_tag="OQM83_002572"
     CDS             5901..7001
                     /gene="lptF"
                     /locus_tag="OQM83_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCS1520811.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            7001..8083
                     /gene="lptG"
                     /locus_tag="OQM83_002573"
     CDS             7001..8083
                     /gene="lptG"
                     /locus_tag="OQM83_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCS1520812.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(8202..9704)
                     /locus_tag="OQM83_002574"
     CDS             complement(8202..9704)
                     /locus_tag="OQM83_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCS1520813.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(9782..10780)
                     /gene="idnR"
                     /locus_tag="OQM83_002575"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             complement(9782..10780)
                     /gene="idnR"
                     /locus_tag="OQM83_002575"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HCS1520814.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            complement(10847..12166)
                     /gene="idnT"
                     /locus_tag="OQM83_002576"
     CDS             complement(10847..12166)
                     /gene="idnT"
                     /locus_tag="OQM83_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HCS1520815.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            complement(12231..12995)
                     /gene="idnO"
                     /locus_tag="OQM83_002577"
     CDS             complement(12231..12995)
                     /gene="idnO"
                     /locus_tag="OQM83_002577"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCS1520816.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            complement(13019..14050)
                     /gene="idnD"
                     /locus_tag="OQM83_002578"
     CDS             complement(13019..14050)
                     /gene="idnD"
                     /locus_tag="OQM83_002578"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HCS1520817.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            14267..14830
                     /gene="idnK"
                     /locus_tag="OQM83_002579"
     CDS             14267..14830
                     /gene="idnK"
                     /locus_tag="OQM83_002579"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCS1520818.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            complement(14834..15853)
                     /gene="ahr"
                     /locus_tag="OQM83_002580"
     CDS             complement(14834..15853)
                     /gene="ahr"
                     /locus_tag="OQM83_002580"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCS1520819.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            16049..16133
                     /locus_tag="OQM83_002581"
     tRNA            16049..16133
                     /locus_tag="OQM83_002581"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16083..16085,aa:Leu,seq:caa)
     gene            16319..>16739
                     /locus_tag="OQM83_002582"
     CDS             16319..>16739
                     /locus_tag="OQM83_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCS1520820.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAEL"
BASE COUNT         4005 a         4346 c         4178 g         4210 t
ORIGIN      
        1 ttgttttcat tggtattttt aatggcactt tatatagtct ggtaatgagt tttctctgga
       61 gtaataccag tttcggtata catcgtttca aggtgaaatt agatactaca tattgtataa
      121 aatatgccat tctcgcattt ttggctttat taccttttct cgctgttgct ggttatatta
      181 tcttcgatca aatattaaat gagtatgata gttctgggta tgcaaatgat gatattgaga
      241 atttacagca atttatggaa atgcaacgta aaatgataat cgcgcagtta atctattatt
      301 ttgggattgc tgttagcacc agttatttaa cggtgtcgtt gcgaaatcat tttatgagca
      361 acctgtcact gaatgatggg cgtattcgtt ttcgctcaac tttaacatac cacggtatgc
      421 tttatcgcat gtgtgcgttg gtggtgatat ccgggattac gggcggtctg gcttatccac
      481 tgctgaaaat atggatgatt gactggcagg caaaaaatac gtatttgctg ggcgatttgg
      541 atgaccttcc tttaatcaat aaagaagaac aaccagataa aggattctta gccagtattt
      601 cacggggaat tatgccttct ttaccatttc tgtaagagat aaaaaaggcc ggagcatgct
      661 ccggccttcg ttttcatcac tgtgttttga ttacagcgcg gcgataacag cctgctgttc
      721 aatcagtttc gctttcgctt ccgcatagcc ttccagcttc tcacgctctt tcgcgatgac
      781 cgcttccggt gcgcgggcga caaagccttc attcgccagc ttgttctcga tacggctgat
      841 ttcgccttcg atcttcgcca cttctttcgc cagacgcgcc agctcatctt ctttgttgat
      901 gaggccagcc atcgggatca gcagctctgc gccgtcgatg atcttcgtaa cggaaaccgg
      961 acctttgtca tcggcaggca gcacggtgat gctttccaga cgcgccaggg tttgcaggaa
     1021 gccacggttt tcatttacgc gacgttctgc atccgcgctg caaccacgca gcagcagctc
     1081 cagcggtttg cccggcgcga tattcatttc tgcacggatg ttacgtaccg caacgatcgc
     1141 ctgtttcagc cattcggtgt cggccagtgc ggcttcatca acctgagatg catcgtactg
     1201 cgggaacggc tgcagcatga tggtgtcggc agtgataccg caaagtactt tcacacgctg
     1261 ccagatggtt tcggtgatga acggaatgat cggatgcgcg aggcgcagca gaccttccag
     1321 tacggtcacc agcgtatggc gagtaccgcg cagttcggct tcggtgccac cgttcattac
     1381 cggcttagtc agctccagat accagtcaca gaactggttc caggtgaact catacagaat
     1441 gcctgcagcg atatcgaagc ggaagctgtc cagcgcttcg cggtacgctt tgatggtctg
     1501 gttgaactct gccagaatcc agcggtccgc cagcgacagc gtcatttcac caccgttgaa
     1561 gccgcaatcc tgaccttccg tgttcatcag cacaaagcgg ctggcgttcc acagcttgtt
     1621 acagaagtta cggtaacctt ccaggcgctt catatcccag ttgatgtcac gaccggtaga
     1681 cgccagcgcc gccagggtga agcgcagggc atcggtgccg tgcggctcaa taccgttcgg
     1741 gaactgcttc tcggtgcgct tacggatttt gtccgccagc tgcggctgca tcatattgcc
     1801 ggtacgtttc tccagcagtt ccggcagcga aataccgtca accatatcca gcgggtcaat
     1861 aacgttaccc ttggatttgg acatcttctg gccttcgtcg tcacgaatca ggccggtcat
     1921 gtaaacggtg tggaacggca cctgcggttt gccattttca tctttgatga agtgcatggt
     1981 catcatgatc atgcgggcaa tccagaagaa aatgatgtcg aagccggata ccatcacgct
     2041 ggttgggtgg aactggcgca gtgcgtcagt gttttccggc cagcctaggg tagagaaggt
     2101 ccacagcgca gaagagaacc aggtgtcgag aacgtcttcg tcctgacgca gggcaacgtc
     2161 ggcaccgagg ttattttcct tgcgcacttc ttcttcgttg cggccaacat aaacgttacc
     2221 tgcttcgtcg taccatgcag ggatacggtg accccaccac agctgacgag agatacacca
     2281 gtcctgaata tcgcgcatcc aggagaagta catgttttcg tactgcttcg gtacgaactg
     2341 aatgtcgccg ttctcaaccg cttcaaccgc cggtttcgcc agtacatcgg cacgcacgta
     2401 ccactggtcg gtcagcattg gttcgataac tacgccgcca cggtcgccgt aaggaacggt
     2461 caggtcgtgc ggtttaattt cttccagcag gccgagtgcg tcaaccgcag caacgactgc
     2521 tttacgtgca gcaaaacgct cgagtttctg gaactctgcc gggatttcgc tggaataaac
     2581 gtcagattcg ttacctttgg tatcgaacac ctgggcgctt tcacggatat cgccgtcaaa
     2641 ggtcaggatg ttgatcatcg gcagcgcgtg acgtttaccc acttcatagt cgttaaagtc
     2701 gtgcgccgga gtgattttca cgcagccggt gcctttttcc atgtcggcgt gttcgtcgcc
     2761 aacgatcgga atacgacggt taaccagcgg tagaatgaca tatttgccaa tcaggtcttt
     2821 gtaacgcgga tcttccgggt taacggccac gccggtatcg cccaacaggg tttccggacg
     2881 ggtagtcgca accaccagat aatctttacc gtctgcggtt ttcgcgccgt cagccagcgg
     2941 atagcggatg tgccacatcg aacctttcga ttcgcggttt tccacttcca ggtcagagat
     3001 agcggtgcgc agtttcggat cccagtttac caggcgtttg ccacggtaaa tcaggtcttc
     3061 tttatacaga cgaacgaaaa cttctttcac cgcattggac aggccttcgt ccatcgtgaa
     3121 gcgttcacgc tcccagtcga cagagttgcc gagacggcgc atctgacggg taatggtgcc
     3181 gccggactcc gctttccatt cccagatttt gtcgatgaaa gcttcgcggc cgtagtcgtg
     3241 acgggtttta ccttcttctg cggcaatctt gcgctcaacg accatctggg tagcgatccc
     3301 ggcgtggtca gtaccgacct gccatagggt gtttttgccc tgcatgcgct gatagcggat
     3361 catggtatcc atgatggttt gctggaaggc gtgacccata tgcaaactgc cggtgacgtt
     3421 cggcggcggg atcatgatgc agaaactttc ctggctttca tcgccattag gcttaaagta
     3481 gccctgcttt tcccagtgct cgtaaagcgg ctgttcgata tcttgtgggt tatatgtctt
     3541 ttccattatt tccaggttgc cgtattcagg ttgaaaccag ccacgcggta ggctttatag
     3601 cgttcgcgcg ccagttgttt cagagaatct tcataaggaa cgaagtctac cacttctgtg
     3661 aaagcagtgg caaaatctgc aaagcttgtt cgcagactaa tcaatatatc gcgcgggctg
     3721 ctgctgcgtt tttgcggcca ggcgatctcc accggcgcac cgccgcgcgg tccttctccc
     3781 gctaaattat gcgggacaaa gctttctgcc ggacgcgccc acagggcttc atccagccgg
     3841 taagcctgct tttcatcttc acaggcgatg agcacgcgct taccgctgcg ccaacgttct
     3901 gcggcaattt cacacaccag ttgctcaacg gcgcttaagc catcgacggt ggtgtcattg
     3961 tccagaaggt agaacgttgc gtttttcata tatggggctt cttgtggtgg atttaaatat
     4021 tgctgcattc ctcaccctga aagatgagca atgaatttct attgcgctcg atcggttccc
     4081 tctcccttag ggagagggtt agggtgaggg taaaacgcaa acttactctt cgccgttaaa
     4141 cccagcgcgg tttaacagga actgtgccag caacgctacc ggacgaccgg ttgcgccttt
     4201 tgctttacca gaacgccagg cggttcctgc aatatccagg tgcgcccagt tgtacttacg
     4261 ggtaaagcgt gacaggaagc aacctgcggt aatcgcccca ccaggacgac cgccaatgtt
     4321 cgccatatcg gcaaaattgg actccagttg ttcctgatac tcgtcaccca gcggtaagcg
     4381 ccatgcgcgg tcaccggatt gttcagacgc ggcaatcagt tcatgggcca gcggattatg
     4441 gttcgccatc agaccagtaa tatgatgacc cagcgcgatc acgcaggcac cggtcagcgt
     4501 cgccacatca atcaccgctt ccggctcaaa acgttcaacg taagttaaca cgtcgcacag
     4561 taccaggcgg ccttcggcat cggtgttcag cacttcaacg gtttgaccgg acatggtggt
     4621 taacacatcg cccggacgat aggcacgccc gccaggcatg ttttcacagc ctgccagcac
     4681 gccaataacg ttaataggca gttgtagctc tgcgaccatc cgcatcacgc cgtaaaccgc
     4741 tgccgcaccg cacatatcgt acttcatctc atccatgcct tctgaaggct tgatggagat
     4801 accgccggag tcgaaggtta aacctttacc caccagcacg attgggcgtg catcttccga
     4861 cgcgttgcct ttgtactcaa tcaccgacat cagcgattcg ttttgcgaac cctgaccgac
     4921 cgccagataa gagtgcatcc ccagctcttt catctgctgt tcgccgataa cgcgggtgat
     4981 aacattcttg ctgtagctgt cagccagctg gcgcgcttgt gaagcgaggt aagcggcgtt
     5041 acagatattc ggcggcatat tgccgagatc ttttgctgct ttaatcccgg cggcaatcgc
     5101 cagaccgtgc tggatcgcgc gctcaccgct ggtcagttca cggcgggtcg gcacgttgaa
     5161 caccatttta cgcagcggac gacgcggttc gctcttgttc gttttcagct gatcgaaact
     5221 gtagagcgtc tcttttgcag tctcgacagc ctgacgcact ttccagtagt tgttacggcc
     5281 tttaacgtgc agttcagtca gaaagcagac cgcttccatt gagccagtat cattcagcgt
     5341 attaatggtt ttctgaataa cctgcttgta ctgacgttca tccagctcac gttctttgcc
     5401 gcaaccaata aggagaattc gctcggaaag tacattcgga acatggtgca gcaacagtgt
     5461 ctgccccggt tttccttcca gttcgccccg acgtagcagg gcgctgatgt acccatcgct
     5521 gattttatcg agctgttctg caatcggaga aaggcgacgt ggttcgaaga cgcccacgac
     5581 gatgcaggca ctccgctgtt tctccgggct accgcttttt acactaaact ccatgcacta
     5641 cgctcctgaa tcttaaagac aacggcggtg gctacagata gaattgcaag ctttcgtaac
     5701 tcatgtccgc tgttgcgatg acttcgtgtt aatcttaacg ttattacggc attggcacgt
     5761 cagaacaaaa tctgagaggt gaatccgttg agtataatta tcttagcgac gatttcgacg
     5821 actcaagaga ataaatgacg tttaagccat gaaacaagct aaaatcctgc aaaagacgag
     5881 tttttacggg cgtatttaaa gtgataatca taagatatct ggtgcgggag acgctcaaaa
     5941 gccagctggc gatactcttc atcttgcttt tgatcttctt ctgtcaaaag ttagtgagga
     6001 tcctcggcgc agcggttgac ggcgatattc cggcgaatct ggtgctctcc cttctcgggt
     6061 tgggcgtgcc ggaaatggcg cagcttatcc tgccattaag cctgttcctc gggctgctga
     6121 tgacgctggg caaactgtat accgaaagtg aaattacggt aatgcatgcc tgcggtctga
     6181 gcaaagcggt gctggtgaaa gcggcaatga tccttgcggt gttcacggca atcgtcgcgg
     6241 cggttaacgt gatgtgggcg ggaccatggt catcgcgtca tcaggatgaa gtgttagcag
     6301 aagcgaaagc gaaccctggc atggcggcgc tggcgcaagg gcaattccag caagcgacta
     6361 atggcagctc ggtgctgttc atcgaaagcg ttgacggcag cgatttcaaa gatgtgttcc
     6421 tcgcacaaat tcgaccaaaa ggtaatgcac gtccttctgt ggtggtggcc gattccggac
     6481 atttaaccca gctgcgcgac ggctcccagg tcgtcactct caaccaggga acgcgcttcg
     6541 aaggcactgc actgttacgc gatttccgca ttacggactt tcaggattat caggcgatca
     6601 ttggtcacca ggcggtggtg ctcgacccga acgataccga ccagatggac atgcgcacat
     6661 tgtggaacac tgacaccgat cgtgcccgcg cagaactgaa ctggcgtatc acgttggtat
     6721 tcaccgtgtt tatgatggca cttatggttg taccgttgag cgtggttaac ccgcgtcagg
     6781 gacgcgtact gtcgatgctg ccagccatgc tgctgtatct acttttcttc ctgatccaga
     6841 cctccctgaa atcgaacggc ggtaaaggta agctggaccc gacgctgtgg atgtggaccg
     6901 ttaacctgat ttatctggct ttggcgattg ttctcaacct ttgggacacc gtgccggtcc
     6961 gccgcctgcg cgccagtttt tcgcgtaaag gagcggtgtg atgcaacctt ttggcgtact
     7021 tgaccgctat atcggtaaaa ctattttcac caccatcatg atgacgctgt tcatgctggt
     7081 gtcgctgtcg ggcattatca agtttgtcga tcagctgaaa aaagccgggc aggggagtta
     7141 cgacgcgtta ggcgcaggaa tgtatacctt gctgagcgtg ccgaaagatg tgcaaatctt
     7201 cttcccgatg gcggctctgc ttggggcgtt gcttggtctt gggatgctgg cgcagcgcag
     7261 cgaactggtg gtgatgcagg cttctggttt tacccgtatg caggtggcgc tgtcggtgat
     7321 gaaaaccgcc attccgctgg tcttgctgac gatggcgatt ggtgaatggg tcgcgtcgca
     7381 gggcgagcag atggcgcgta actaccgtgc gcaggcgatg tacggcggct cgttgctctc
     7441 cactcagcaa ggcttatggg cgaaagatgg caacaacttc gtctacattg agcgggttaa
     7501 aggtgacgaa gagttaggtg gcatcagtat ttatgccttt aacgagaatc gtcgtctgca
     7561 atccgtacgt tatgccgcta ccgcgaagtt tgacccggaa cataaagtct ggcgtctgtc
     7621 gcaggttgat gaatctgatc tgaccaatcc gaaacagatc accggttcgc agacggtgag
     7681 cggcacctgg aaaaccaacc tcacgccaga caaactgggc gtggtggcgc tggacccgga
     7741 tgcactctcc atcagtggtt tgcacaacta tgtgaagtat ctgaagtcga gcggtcagga
     7801 tgccggacgt tatcagctca acatgtggag caaaatcttc cagccgctat ccgtggcggt
     7861 gatgatgttg atggcgctgt cgttcatctt tggcccactg cgtagcgtac cgatgggcgt
     7921 gcgtgtggtc accggtatca gcttcggttt tgtcttctac gtactggacc agatcttcgg
     7981 cccgctgacg ttggtttatg gcatcccgcc gatcatcggc gcactgttgc caagcgccag
     8041 cttcttctta atcagcctgt ggctgttaat gagaaaatcg taatctaaca agggagcgaa
     8101 agctcccttt attattgttg acaaagtcaa atacgagttc gaacggtccc ctcgcccctt
     8161 tggggagagg gttagggtga ggggcacaga tcacgtctga cttacctttt tctccccccc
     8221 agcaaactcc ccaacatccc gcgtacaatc tgattcgtca cctggcgagc ggcgcttttc
     8281 gccattgttt gcaccacacc atctttcttc ccgccgcgtg gtccggtagt gccaaacaaa
     8341 atatccttca atccaccaag aataccgtca tccaccgcca cctctttccc tttcacgggg
     8401 ggattatttt gctgctcggt actggcctga aagccttttt gcagcatctc ataggcggac
     8461 tcgcggtcca cctcatcttc atatttgcca tacaccgggg agtgattaat caggccatta
     8521 cgctcatctt ccgtcaccgg ccccattcgc gaacaaggtg cgatcaccat cgcccgttcc
     8581 accactgaag gacttccttt cgcatcgaga aacgagatta acgcctcgcc ggtccccagt
     8641 tcctggattg ccttttcggt atcaaacgcc ggattggccc gcatggtttg cgccgctgcc
     8701 ttcactgctt tctgatcttt aggcgtaaaa gcacgcaaag cgtgttgaac gcgattaccg
     8761 agctgcccta gcacgttatc cgggatatct gacgggtttt gcgaaacgaa ccagacgccc
     8821 acgccttttg agcgaataag ccttatcacc tgctcaatct tatccagcag tacctgtggc
     8881 gcatcgttaa acagcagatg tgcttcgtcg aagaaaaaca ccagttttgg tttctccaga
     8941 tcgcccgctt caggcaattg ttcatataac tctgaaagca tccacagcag gctggcggcg
     9001 tacagtttcg gcatctgata aagtttctcg gcgctgagga tattgataac gcctttaccg
     9061 ttggcatcgg tgcgcatcca gtctttgata tccagcatcg gctcaccaaa gaagtgttct
     9121 gcgccttgct gttccagcga cagtaatccg cgctggatgg caccaaccga tgcgctactg
     9181 atatttccgt actgattctg gaaggatttg gcgttatcgc cgatgtactg ggtaatcgcc
     9241 cgcaaatctt taaagtcgag cagcaacaat ccctgatcgt ccgcaatgcg gaagataata
     9301 ttcagcacgc cagattgcac atcgttgaga ttcaacagtc gtgccagcaa cagcggcccc
     9361 agatccgaaa ccgtcgcccg caccggatgg cctttctcgc caaagatatc ccacaccacc
     9421 accggattgg catgcggttg ccagtcgttg acgccgatat ttttcagtct tgcgagcagt
     9481 ttttccgacg ccgttcctgc ctgcgcgatg ccggtcagat cgcctttcac atcggccata
     9541 aacaccggaa caccgatttc cgacaatgac tctgccagtt tttgcaacgt gacagtttta
     9601 cccgtccccg ttgcgccagt aatcagcccg tgacggttag ccattcccgg cagtaaaaac
     9661 agttctgtgt ccggcgtgcg ggcaattaac aggggttcgc tcatgagatt tcctccagtt
     9721 tcctgcctgg agtataggca acgtccggca aagagaatat gtgtaatgaa gagaaaataa
     9781 cctaaagggt gttaccgtga taaatctgat acccgaggtc aacagtgttg tggttgtgat
     9841 cgttattctt aatcttgctc agcaacattt gggcggccat ccgaccaata tcaaaacggg
     9901 gagtaattac gctggcgagg cttgggatca tttgcctgcc aatctccagc ccatgaaaac
     9961 ccgcaatgga aatttgttct ggcacggcga ggtttcgctc gcggcacagc agaagcgcac
    10021 ccatcgcaat atcgtcattg gtgcaaaata caccgtctaa atccgggttt gcgctaaggg
    10081 catcacgcat taactgcatt cctaaatgaa tggatgagat ggcgcgtgga ttcatgcgta
    10141 atggggaaag attatgcagc atcatcgcat cgcaataccc ctgataacgc tgttcatcgc
    10201 gggtgtcatc tttggaaccg agatacagaa ttttgcgcct gactcgctta tccagcatgg
    10261 tacacaccat gtcaaaagca gcctgccgat tatcaaaacc gacctccata tccagccgtt
    10321 ctccctgtac atccatcaat tccacgaccg gaatggtggc tgaacgcaga aatttcaccg
    10381 tcctgatggt gtgatatttt tccgaaagaa taatcccgtc aatgttataa gagagcaggt
    10441 taatcaccga ctcttcttcg gaatcgcgat cgtaattgta attagcaata agggtctgat
    10501 aattatgcac agaggtgacg gattctattc ccgcgagaat gtcagcgaag agttgatttt
    10561 gaaatgacgg aatcaagata ccgagggtat aactttgtgc attcaacaac atgccgggag
    10621 cgcgattagg gatataattg atctcttcca taattttggc gatgcgctcg cctgtttcct
    10681 ttgccacctt tttcggcgag cggatataac gactcacggt catttttgtt acgccagcca
    10741 gcgtagcgat atcctgtaaa gaaattctgt gattgcgcat aacttctccc aacgtcgggg
    10801 gaatcttcaa tcttcccccg aaactacatc acaatatttt attctttcag tgcaatacgg
    10861 cgttaatggc taacacgccc agcaaaccca taatagaaat aagggtttcc atcactgtcc
    10921 aggtacgcaa cgtttcacca accgtcagat taaaataccc tttaaatagc cagaagccag
    10981 ggtcgtttac gtgtgacgcg atcacgctgc ccgcaccagt tgccagtacc attaaagcgg
    11041 gatcggcatg ggtaacgttg ataatcggca acaccacacc cgcggtggta atggccgcga
    11101 cggtggcaga gcccagagcg atacgcaaca gcgccgcgac agtccagcac atcaataacg
    11161 gcgaaagcgt agttccggtc attaagtgtg aaatatactg cccgacacca ctatctacta
    11221 atacctgctt aaacgcgccg ccgccagcga taataaacac aatcatcgcg atagcgccta
    11281 tagaatcccc aatgatatcc atgatttgct cgatggtgcg tccattacgt cggcccagtg
    11341 tgaaaatcgc aataacaatg gcaataaaca gcgcaacggc agggttaccg acaaattcaa
    11401 agaagaggcg cacggtgtta gttttcggta acgtaatttc acaaacggcg gcgatagcca
    11461 tcaggatgac cgggatcact gcagcaaaaa tactgttcca gaaggagggc atctcctctt
    11521 cgctaaacag atgaggatta aataagcctt ccggtggtgc tttctcaaag cgagttagca
    11581 gtttagaaaa cagcggtcct gcgacaataa ctgtcggaat ggtaatgata aatccataca
    11641 gtaaagtcgt tccgagatta gcctcaaaga tagtcgcgat ggcagtaggc cctggatgtg
    11701 gcggcagaaa acagtgggtt acagagagcg ctgctaccat cggtacgcca acatacaaca
    11761 ggggtaatcc agatgatgcc acgatggtaa ataccaacgg caacagcagg acaaaaccca
    11821 cttcaaaaaa catggcgagg cccacaacca gaccggtgat cactagcgcc cattgcacgc
    11881 gttttttacc aaaagtagca atcagcgtag tggcgatacg ttgtgccgcg cccgtatcag
    11941 aaatcagctt gcctaacatg gccccgaaac cgaggatcat tgccagcccg ccgagcgtgc
    12001 tgccgatacc attttgtata gagtgcagga cggcctgtgc atccatccct tcggcaaatc
    12061 cgacgacggc agctaccaga acgagggcaa taaagccgtt aactttaaag ccgatcatca
    12121 ggataagaag cagcgcgacg cctgccgcaa taatgattaa tggcatagtc tttatctctt
    12181 attagtgcca gaaaagcagc gctttcgcgc tgcacgtagg gtggtggctg ttaaacagcc
    12241 actaacatgc cgccatcaac aaacaacagg tggccgttta cgaaatcaga ggcttttgaa
    12301 gaaaggaaca ccgcagcacc aatcagttcc tgcggatctc cccagcgtgc ggcgggggtc
    12361 cgtttgcaca accaggcggt gaaggcttcg tcctcaacca gtgctttagt catttctgtt
    12421 ttgaaatagc ccggcgcaat accgttgacc tgaatattgt ggcgcgccag ctcgacgcac
    12481 atgccgcggg tgagcatttt taccgcccct ttcgatgcgg cataaggggt gatggtgtca
    12541 cgtcccagtt cgctttgcat cgagcaaata ttaataactt tacctgcctt gcgttcaacc
    12601 atgtgacgag ttaccgcttg cgataccagg aacacggcgg tctggtttac tgcgatcaca
    12661 tcattccact cttgttcagg gaactcagta aaaggatgac ggcgctggat accggcgtta
    12721 ttcaccagca catcaatggg gccgatgtcc ttttcgatat gttcaacggc ggcatcaatt
    12781 tcatgtttat gagtaacatt aaaaggtgcg gcaacggcct gaataccctc ctggtggagt
    12841 ttttctacag caagttctgc gcgttcggca gtaatatcat taataattat ttgtgcgcca
    12901 tatttaccca ggccggttgc cagtaaatag ccaatgccct gtgctgaacc ggtaatcaag
    12961 atattttttc ctgccagtga aaatagatcg ttcattgtat tattccttta tttatctctt
    13021 agaaaacaag ctggactttt gctgcctggg ttttatcacc ggcgaaacgt agcgcctctt
    13081 ccaggtcagt gaagagatat tcagcactca gtaaaggcag tggattgata acgccattcg
    13141 ccagccatga cactgcggta ttaaattcgc tggtaaaacg gaaagagcct ttgagtgaaa
    13201 tctccttacc aatcaacgtc atcattggga attctgccat cgcgcctccc atccctacct
    13261 gcaccattac gccgcgtgca cgagtgacct ccagacaggt attcactgat gaaggatgac
    13321 cggacacttc aaagctgaca tcgaaatggc ctttttccgc tttccagtga tccatgtcgt
    13381 cgttttgtgg gtttacgagc acatccgcac ccatctcttt ggccagcgaa agggaacggg
    13441 gactcacatc ggcacagaca atttccgcgg accccagtgt tttcacagca ctgacaatca
    13501 ggcagccaat gggcccaaca ccggaaacaa atactcgctt gccctgtaac tcgtcggcct
    13561 gatgtgcggc atgaatcgcg acggctaaag gttcggcaaa agccataacc ttttcgtcag
    13621 ctttggccgg ataagggaca cattgcgacg tttcgaccat tttataacgg gtaaaaccac
    13681 catcaacatg agggaaatac atggcactgc caaaaaaacg catatctgta cactgattct
    13741 cgttatgttc aatgcagtat ttgcagtgac cgcacggttt agacggatta atggctaccg
    13801 tttgcccttc atgtaattct gatgagtcgc tatgaataac tttaccgata acttcatgac
    13861 ctaacaccat cggtgccttt atcatgaaat tacctacttt tccttcctga taataatgta
    13921 aatcggaacc gcaaattcca cctcgggtta tttgtactaa tgttccatta ttattccaat
    13981 ctatcgtctg ctcggtaacg gcaacagttt tcttgtccgc aacaacgcag gactgtgttt
    14041 tcacttgcat aaattcctca ctggtcaggt agacacctcg gaagcattta agcggtttta
    14101 attgtcatta tttgtgatga agatcacgtc agaaaattgt tacattacta tgttacgcat
    14161 aacgtgatgt gccttgtaat tcttatcagc agaaataaaa aaacgtgaaa ttattatgcc
    14221 accaggcgta gtatcgcagc aggtaagatg attcaggaga ttttaaatgg cgggtgaaag
    14281 ctttattttg atgggcgttt cagggagtgg taaaacatta attggtagca aggttgccgc
    14341 gttattatct gctaaattta ttgatggtga cgatcttcat ccagccaaaa atatagataa
    14401 aatgtcgcag ggtattccat tatctgatga agatcgactt ccctggctgg aacgcttaaa
    14461 tgatgcttca tacagtcttt ataaaaagaa tgaaacagga tttattgtct gttcatcatt
    14521 aaaaaaacag tatcgtgata ttttacgcaa gggtagcccc catgttcatt tcctctggtt
    14581 agatggcgac tatgaaacta ttctcgcgcg aatgcagcgg cgggctgggc attttatgcc
    14641 ggtagcgtta ctaaaaagtc agtttgaggc gctggagcgt ccacaagcag atgaacagga
    14701 tattgtacgc attgatatca accatgatat tgccaatgtc accgaacaat gtcggcaggc
    14761 cgtgctggcg atacgacaag acagaatatg tgcgaaaggg ggtagcgcct cagatcagcg
    14821 ctgcgaatga ttttcaaaaa tcggctttca gaaccacgcg gtaacgcgcc ttaccgttgc
    14881 gcacatgctg gatggcgtcg tttattttcg acatcgggaa cagttcggtg gtcggcgcaa
    14941 ccttgctgcg ggccgcaaag cgcatcagct tacgaagctc ataaggtgtg ccggttgctg
    15001 aaccagagac gctgcggtcg cccgcaatta acgtaaaggc cggaacagac agcggcgtga
    15061 ggaccgcacc gacggtgtgg aaattaccgc catacgtcag cgcctcaaaa taaggctgcc
    15121 agtcgaggct gacgttgacg gtgttgataa tgagatcgaa ctgccccgca agtgttttca
    15181 gtgcctgcgg atcgcggcta ttcaccactt tatcggcacc catcgccagt acttcctgct
    15241 ctttcgcagg attcgaacta aaggctgtca cctcgcatcc cattgcgtgc agaagtttta
    15301 tagcgatatg ccccagcccg ccaataccaa ttaccccaac gcggctggta gcagtgatat
    15361 ggtgcatcaa cagtggttta aagaccgtga taccaccaca cagcagcggc ccggcggact
    15421 cgatatcgat attttccggc agtgggatca cccattgcca gtctgcacgc aacttctcgg
    15481 caaaaccgcc gcgattcata attgttggca ccgcaccttg ctcgcagttg atctgattac
    15541 cgctaataca ggcgtcgcaa tgcccgcagc tacgcgccgt ccagccaatc ccgacacgct
    15601 gaccgacctg caaaccttta tcctgcgcgg cgctcccgag cgccaccacg cgcccaatca
    15661 cctcatgccc ggcaaccagc ggatactgtg aaaatcccca ttcgttgtcg atcatcgaca
    15721 gatcggaatg gcagatcccg cagtaatcca cctgcacttc aacatcttgt ggcttcaatt
    15781 caccgggctc gtactcataa acttccagtt cgccgcccgc ttcttttgcg gcatagcttt
    15841 ttatcatcga cattttttgg tccttctctg gtgttgtctg ggaagtgtag agcatggcag
    15901 ggcggggtgc ctggagtgtg acaaaggtta cacatcgctg tatgcaatgc tgaaaatttc
    15961 agcacttagc gaggtgcgag caagctggcg cttgcatggt ggcgtgcgac aggtataatc
    16021 cacaacgttt tccgcatacc tcttcagtgc cgaagtggcg aaatcggtag acgcagttga
    16081 ttcaaaatca accgtagaaa tacgtgccgg ttcgagtccg gccttcggca ccaaaagtat
    16141 gtcaatagac ctcaactgag gtctttttta tgcctgaaat tcagtattta tctacctttc
    16201 ccgctatatt aactctctca aggtcaaccg atatcaacgt acttctacca acatatgttg
    16261 gtacagatga tggtatttcc ggttcgataa tgcttgtacc aacagggagg gaatacgcat
    16321 ggcattaaca gacatcaaag tcagaacagc taagccaaca gataagcaat ataagttgac
    16381 tgatggtggc ggtatgcatc tgcttgtcca tccaaatggt tctaagtact ggcgtttgca
    16441 gtaccgttat gagggaaagc aaaagatgct ggcacttggg gtttatcctg aaatcacact
    16501 cgcggatgcc agaatacgtc gtgacgagcc acgtaagctg cttgcgaatg gcgtcgatcc
    16561 gggagacaaa aagaaaagtg ataaggttga acagagtaaa gcacgaacct ttaaagaagt
    16621 cgcgattgag tggcatggca ccaataaaaa gtggtctgaa gatcatgccc atcgtgtgct
    16681 aaaaagtctg gaagataatc tttttgcagc gcttggtgaa cgtaatatcg ctgagttaa
//
LOCUS       DAJVQG010000089        16554 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJVQG010000089 DAJVQG010000000
VERSION     DAJVQG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16554
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.089"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="OQM83_002583"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="OQM83_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCS1520821.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="OQM83_002584"
     CDS             433..621
                     /locus_tag="OQM83_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCS1520822.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="OQM83_002585"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="OQM83_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCS1520823.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="OQM83_002586"
     CDS             816..989
                     /locus_tag="OQM83_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCS1520824.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="OQM83_002587"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="OQM83_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="OQM83_002588"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="OQM83_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HCS1520825.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCLSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="OQM83_002589"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="OQM83_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCS1520826.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="OQM83_002590"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="OQM83_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCS1520827.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="OQM83_002591"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="OQM83_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCS1520828.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKDEK"
     gene            7995..10406
                     /gene="torA"
                     /locus_tag="OQM83_002592"
                     /pseudo
     CDS             7995..10406
                     /gene="torA"
                     /locus_tag="OQM83_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="OQM83_002593"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="OQM83_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCS1520829.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="OQM83_002594"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="OQM83_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCS1520830.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            12531..13677
                     /locus_tag="OQM83_002595"
                     /pseudo
     CDS             12531..13677
                     /locus_tag="OQM83_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13971..15212
                     /gene="agp"
                     /locus_tag="OQM83_002596"
     CDS             13971..15212
                     /gene="agp"
                     /locus_tag="OQM83_002596"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCS1520831.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(15250..15477)
                     /locus_tag="OQM83_002597"
     CDS             complement(15250..15477)
                     /locus_tag="OQM83_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCS1520832.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(15498..16103)
                     /gene="wrbA"
                     /locus_tag="OQM83_002598"
     CDS             complement(15498..16103)
                     /gene="wrbA"
                     /locus_tag="OQM83_002598"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCS1520833.1"
                     /translation="MSVKARILVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPE
                     TMPPQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWA
                     SGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGG
                     TPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            16155..>16554
                     /locus_tag="OQM83_002599"
     CDS             16155..>16554
                     /locus_tag="OQM83_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1520834.1"
                     /translation="MKLLVTYIRWSTKEQDSGDSLRRQTNLIDAFYSKHKNDYYLLPA
                     HRYVDKGKSGFHQQHKNQGSDFRRMFENVMSGAIPEGSLIVVENFDRFSRADIDTAID
                     DVRQILRKGVSILTLGDGELYDKSALTDPVK"
BASE COUNT         4159 a         4071 c         4226 g         4098 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgcggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggt gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcaag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gcggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggcat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgcgtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatcgc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgccatcaa gatgggttgc
    12421 tgaacatatt ttaaacaggt gaaaaagggt gagcgatttt tgatagttga accaggcact
    12481 ttaagtttaa ctagggcgtc attatttatt aaattttata gacgctatat atgggtagta
    12541 atatacatgg aattagttgc actgcaaata attatttgaa acaggcctgg aacgatataa
    12601 aaaatgagta cgaaaaaaat caaacatatt caatcacgct ttttgaaaac acactggtgt
    12661 gttttatgcg gttatacaat gaactcagac gtaaagtaaa tgaagaggat actccatgtc
    12721 tggaatgtga atcactagaa aaagaatttg aggaaatgca gaatgataat gatctatcat
    12781 tatttatgag aacattgcgt actaatgata cacaaattta ttcaggggtt tcaggaggta
    12841 ttacatatac tatacaatat gttcaaaatg ttgatattgt tagagtgtcc ttgccgggca
    12901 gaggttcaga gtctatcaca gattttaaag gttattattg gtatggcttt atggaatata
    12961 ttgaaaacat tactgcgtgt gatgacattt tttctgagta ttgtttagat gatgaaaata
    13021 tgagtatcca gccagaacag ataaatacgc cgggaatatc tgatttagat actggtttta
    13081 aaagaacatg agtattcatg ggcgctgggc ggccataaga cgcaaaagca agtatacgaa
    13141 gagtttatta aaattgttga taaacaaacg atcgaggctc ttgttgatac ggcattctat
    13201 aatctcaggg aagatgtaaa gacgttattt ggcgttaatc tacaatctga caacaaaatt
    13261 caggggcagg gtggtttttt atcatggagt tttttgtctt ttaaaaaaga atttattgat
    13321 agttgcttga atgacaaaaa atgcatactg catttacccg aatttatatt taatgataac
    13381 aaggcacgcc ttgttttaga caccgaccca gagcagaagg ttaatgaggt gaaaaatttc
    13441 ctcactgctc tttcagatag catttgctca ttatttattg tttacagcaa tgtggcatca
    13501 atctccttag ggaatgaatc cttttcaaca gatgacgacc ttgagtatgg gtatttaata
    13561 aacactggca atcattatga cgtttacctc cctcctgaac tttttgctca ggcttacgag
    13621 ttaaacaata aggaaaggaa tgcgcaaatt gattttttaa ctcgttatgc aatttaatga
    13681 caaaggcatg tgctaaaaac cattgttatt agcctcactt ctcttattat tggtactatt
    13741 gcctctgtat tagtaacgcc gctgatattc tgtttatcca caggtgcaat tatcagcggc
    13801 gtacgcgagg caggggctaa tcaggcatag tttgcgtcaa accttgcctg tttttgaaga
    13861 tgtatataga aaaacaggcg ttcaacaagc cattttgcga acctgttccc ggaaaaaagt
    13921 catatttctg tcacactctt taatgattga taacaaaaga ggtgccagga atgaacaaaa
    13981 cgctaatcgc cgcaactgtg gcagggatag ttttactcgc ttcaaatgct caggcacaaa
    14041 ccgtaccgga aggctatcag ctacagcaag tgctcatgat gagccgccat aacttacgtg
    14101 cgccgctggc gaacaatggc agtgtgctgg agcagtcgac gccgaataaa tggccagaat
    14161 gggacgtccc cggtgggcaa ctcaccacca aaggtggcgt gctcgaagtg tatatgggcc
    14221 attacatgcg tgaatggctg gcagagcagg ggatggtgaa atcgggggaa tgcccgccgc
    14281 cggacaccgt ttatgcctat gccaatagtc tgcaacgtac cgttgcgacc gcacagttct
    14341 ttattaccgg cgcattcccg gggtgtgata ttcctgtgca tcaccaggaa aaaatgggca
    14401 ctatggaccc aacctttaac ccggtgatca ccgatgattc cgccgcattc agtgaacagg
    14461 cggtggcggc aatggagaaa gagctcagca aactccagct taccgacagc taccagctac
    14521 tggaaaaaat cgttaactat aaagattccc ctgcctgtaa agagaaacaa cagtgttcgc
    14581 tggtggatgg caaaaatacc tttagcgcca agtatcaaca agaaccaggt gtttccgggc
    14641 cgctgaaagt cggcaactcg ctggtagatg cgtttacttt gcaatattac gaaggttttc
    14701 cgatggatca ggtggcctgg ggagaaatca aatctgacca gcagtggaag gtgttgtcga
    14761 agcttaaaaa cggctaccag gacagcctgt ttacctcacc ggaagtggcg cgcaatgttg
    14821 cgaaaccgct ggtcagttat atcgacaaag ctctggtcac cgatcgcacc agcgcaccga
    14881 aaattacagt gttggttggg cacgactcca acattgcctc tctgttaacg gcgctggatt
    14941 tcaaaccgta tcagttgcat gaccagaacg aacgcacgcc gattggcggc aaaatcgttt
    15001 tccagcgttg gcatgacagc aaagccaatc gcgatttgat gaaaattgaa tatgtgtatc
    15061 agagtgcgga acagttacgt aatgccgatg cgttaaccct gcaggcacct gctcagcgtg
    15121 tgacgctgga attaagcggt tgcccgatag atgccaacgg tttctgcccg atggataaat
    15181 ttgatagcgt gttgaatgaa gcggtgaaat aacagaaaac tcccccgcga gaagcggggg
    15241 agtcgctggt taaacgtttt tacgttcgat ggtctgttcg ccccaaaaaa gcgaatcttt
    15301 atcggcctta gcaaaggctt tgactaacac ttcatcgcta ccttcttccc aaatcttttc
    15361 tgccattttt tcgtcgtacc cggcgacttc gaaaatggcc tcggctattt ccggcgacgt
    15421 attgcgcaga gatgcccatt caccgacgtg atgagctttc gcttcttgag ttggcatgcg
    15481 tatcctcctg ttgaagatta gccgttaagt ttaactgcca gacctgcgac atattcccct
    15541 tgataacgag caatagacag ttcttcctgg cttggctggc gtgagccgtc accgcctgcg
    15601 atggtggttg cgccgtacgg cgtaccgccg cgaacctgtg aaacgtcaaa taattcctgc
    15661 gctgcgtagc caatggggac aatcaccatg ccgtgatgcg caagggtcgt ccaggttgaa
    15721 gtaatagttt gttcctgacc gccgccagta ccggtggaac taaagacgct cgccagtttt
    15781 ccgtatagtg cgccggaagc ccacaggccg cccgtctggt cgaggaaggt acgcatttga
    15841 ccggacatgt tgccaaagcg ggtaggtgta ccaaaaataa tggcgtcgta atcggccagt
    15901 tcttgcgggg ttgcaaccgg tgcagtttgc gttttaccgc ctgctttttc aaataattgc
    15961 ggcggcatgg tttccggtac acgcttaacg acaacttctg cgccatcgac tttgcttgca
    16021 ccctcagcga ctgcgcgtgc catcgtttca atatgtccgt acatggaata ataaagcact
    16081 agtattcttg ccttaacact catatcattg ttctcctgtt caatgaacca cttagataag
    16141 catagtccga agatatgaag ctgctagtga catacatcag gtggagtact aaagagcaag
    16201 actcaggaga ctccttaaga cggcaaacaa acctcattga tgctttctac tctaagcaca
    16261 aaaacgatta ttaccttctt cctgctcaca gatatgttga taagggtaaa tctggttttc
    16321 accagcaaca taagaatcaa ggttctgact tcagacgtat gtttgaaaat gttatgtctg
    16381 gggctattcc agaaggttcc ttgattgttg tagagaactt tgaccgcttc agtagggctg
    16441 atatcgacac agctatagat gatgtcaggc agatactgag gaagggggtc tctatactga
    16501 ctctgggtga tggtgaactc tacgacaagt cagccttaac agaccctgtg aagt
//
LOCUS       DAJVQG010000090        16501 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJVQG010000090 DAJVQG010000000
VERSION     DAJVQG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16501
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.090"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            192..2039
                     /gene="ldtD"
                     /locus_tag="OQM83_002600"
     CDS             192..2039
                     /gene="ldtD"
                     /locus_tag="OQM83_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1520835.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2220..2768
                     /locus_tag="OQM83_002601"
     CDS             2220..2768
                     /locus_tag="OQM83_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCS1520836.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2795..3442
                     /gene="gloC"
                     /locus_tag="OQM83_002602"
     CDS             2795..3442
                     /gene="gloC"
                     /locus_tag="OQM83_002602"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCS1520837.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OQM83_002603"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OQM83_002603"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCS1520838.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSSLINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OQM83_002604"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OQM83_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCS1520839.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="OQM83_002605"
     CDS             complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="OQM83_002605"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCS1520840.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8301..9503)
                     /gene="pncB"
                     /locus_tag="OQM83_002606"
     CDS             complement(8301..9503)
                     /gene="pncB"
                     /locus_tag="OQM83_002606"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCS1520841.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9769..12381
                     /gene="pepN"
                     /locus_tag="OQM83_002607"
     CDS             9769..12381
                     /gene="pepN"
                     /locus_tag="OQM83_002607"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCS1520842.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLEEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12588..13355)
                     /gene="ssuB"
                     /locus_tag="OQM83_002608"
     CDS             complement(12588..13355)
                     /gene="ssuB"
                     /locus_tag="OQM83_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS1520843.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13352..14143)
                     /gene="ssuC"
                     /locus_tag="OQM83_002609"
     CDS             complement(13352..14143)
                     /gene="ssuC"
                     /locus_tag="OQM83_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCS1520844.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(14155..15302)
                     /gene="ssuD"
                     /locus_tag="OQM83_002610"
                     /pseudo
     CDS             complement(14155..15302)
                     /gene="ssuD"
                     /locus_tag="OQM83_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(15299..16258)
                     /gene="ssuA"
                     /locus_tag="OQM83_002611"
                     /pseudo
     CDS             complement(15299..16258)
                     /gene="ssuA"
                     /locus_tag="OQM83_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
     gene            complement(16251..>16501)
                     /locus_tag="OQM83_002612"
     CDS             complement(16251..>16501)
                     /locus_tag="OQM83_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCS1520845.1"
                     /translation="TVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQF
                     TPNLQTRLDTALETFWQALHRRDVQVPDLLSLRGNVHA"
BASE COUNT         4120 a         4297 c         4206 g         3878 t
ORIGIN      
        1 attacggcgc gcttttcttt aaacttcttt acattaggtt atgtaaaaac gtatcggcgt
       61 ttatatactg aagataagcc tgatgagtaa caggcttgct cgtcatactt tcgtgagtat
      121 tggcgttgta caggcaagtc gtaaaataac agcctggcta ttcagagtat gataaaaaca
      181 gggggcaagg gatgttgctt aatatgatgt gtggtcgtcg gctgtcggca atcagtttgt
      241 gcctggccgt aacattcgct ccactgttca atgcgcaggc cgatgagcct gaagtaatcc
      301 ctggcgacag cccggtggct gtcagtgaac agggcgaggc actgccgcag gcgcaagcca
      361 cggcaataat ggcggggatc cagccattgc ctgaaggtgc ggcagaaaaa gcccgcacgc
      421 aaatcgaatc tcaattaccc gcaggttaca agccggttta tcttaaccag cttcaactgt
      481 tgtatgccgc acgcgatatg caacccatgt gggaaaaccg tgatgctgtt aaagccttcc
      541 agcaacagct ggcagaggtg gcgattgccg ttttccagcc gcagtttaat aaatgggtag
      601 agttactgac cgatcctggt gttaacggga tggcacgcga cgtagtgctc tctgatgcga
      661 tgatgggcta tctccatttc attgcaaata ttccggtcaa aggcactcgc tggctatata
      721 gcagtaaacc ttatgcactt gcaacgccgc cgctctcggt gattaaccaa tggcagctgg
      781 cgctggataa aggtcaattg cctacgtttg ttgcaggact ggcaccgcag catccgcaat
      841 atgcggcgat gcatgaatcg ttactggcct tactcagtga caccaaaccg tggccccaac
      901 tgaccggcaa agcaacgttg cgcccagggc agtggagtaa cgacgtaccg gcgttgcgcg
      961 aaatattgca acgcacaggc atgttggacg gggggccgaa aattactcta cctggcgatg
     1021 acacgccaac tgacgcggta gtcagcccat ccgctgttac tgttgaaaca gcagaaacta
     1081 agccgatgga caagcaaacg acgtctcgta gtaaacctgc gcctgccgtt cgcgccgcct
     1141 acgataatga actggtggaa gccgttaaac gttttcaggc atggcaagga ttgggggcag
     1201 atggtgctat tggcccggca acacgtgact ggttaaacgt aacgcccgcc cagcgtgctg
     1261 gcgtgttggc tctcaacatc cagcgattgc gcttgctgcc aacagagctt tctaccggga
     1321 tcatggttaa cattccggcc tattcgctgg tctactatca gaacggcaat caggtgctgg
     1381 attcgcgagt cattgtcggt cgccccgatc gcaaaacgcc gatgatgagc agtgccctta
     1441 acaacgtagt ggtaaacccg ccgtggaacg tacctccaac tctggcacgc aaagatattc
     1501 tgccaaaagt gcgcaacgat ccgggatatc tcgaaagcca tggctatacg gtgatgcgcg
     1561 gctggaacag cagagaagcg attgacccat ggcaggttga ctggtctaca atcacggcct
     1621 cgaatttacc gttccgcttc cagcaggctc caggcccacg gaactcgctg gggcgctata
     1681 aattcaatat gccgagttca gaggccattt atttgcatga cacgccgaac cacaatctgt
     1741 tcaagcgtga tacacgcgca ttgagctcag gctgtgtacg agtgaataaa gcttccgatc
     1801 tggcgaatat gctgttgcag gatgcaggct ggaatgacaa acgtatttct gatgcgctga
     1861 agcagggtga tacacgttac gtcaatattc ggcagtcgat tccggtgaat ctctactacc
     1921 tgacggcctt tgttggtgca gatggtcgta cccagtatcg tacagatatt tacaattatg
     1981 atctgcctgc gcgatccagc tcgcaaatcg tatcgaaagc ggaacaatta atcaggtaaa
     2041 tgaagtagtt cggggaaatc agttgtcgta ataattagca tgattggggg cgattctctg
     2101 cagcccccgt cactgctggg gttgagtcat cttgacgtct gctttacggg cggttaaggt
     2161 gcctcttgtg cgccagaagt gcatataaac gataacattg acctgtagac ttgattatca
     2221 tggacaaatt cgacgctaat cgccgcaaat tgctggcgct tggtggcgtt gcactcggtg
     2281 ccgccatcct gccgacccct gcgtttgcaa cactctctac cccacgcccg cgcattttga
     2341 cactcaataa tcttcatacc ggagagtcaa tcaaagcgga gtttttcgat ggcagaggct
     2401 atattcagga agaattggca aaacttaacc attttttccg cgattaccgc gcgaacaaaa
     2461 taaagtccat cgacccagga ttattcgacc agttgtatcg cctgcaaggg ttgttaggca
     2521 cgcgcaaacc ggtgcaactc atttccggtt atcgttctat tgataccaac aatgaactac
     2581 gcgcccgcag ccgtggagta gcgaagaaaa gctatcacac taaaggccag gcgatggatt
     2641 tccatattga aggtatcgcg ttaagcaata ttcgcaaagc cgcgttatct atgcgcgcag
     2701 gtggtgtagg atattatcca cgtagtaact ttgtgcatat tgataccggg ccagcacggc
     2761 actggtagta attgcttaac gaaacagggg cagtatgaac tatcgtatta ttccggtcac
     2821 cgcattctcc cagaactgtt cattaatctg gtgtgaacaa acccgtctgg ccgcactggt
     2881 cgatcctggc ggcgatgcgg aaaaaatcaa acaggaagtt gatgacagcg gcctgacact
     2941 gatgcagatc ctgctgacgc atggtcatct ggaccacgtt ggcgcagcgg cggaactggc
     3001 gcaacattac ggcgtgccgg tttttggccc ggaaaaagaa gatgagttct ggctgcaagg
     3061 cttgcctgcg caaagtcgta tgtttggtct ggaagagtgc cagccgctga cgccagatcg
     3121 ttggctgaac gaaggcgata ccatcagcat agggaatgtg actttacagg tgttacattg
     3181 ccctgggcat acgccgggtc atgtcgtgtt ttttgatgat cgggcaaagc tgctgatttc
     3241 tggcgatgtt attttcaaag gcggagtagg gcgcagtgac ttcccgcgtg gcgatcataa
     3301 tcaactgatt tcttcaatca aagataaatt gctgccactg ggggatgacg tgacatttat
     3361 tccgggtcac ggaccattat ccacacttgg ttatgaacgc ctgcataatc ccttcctgca
     3421 agacgaaatg cccgtctggt aaggcacata aaaaagcccg cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 tgcttacagc actgccacaa tcgcttcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaaccgc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcaggc cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcgcctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaacg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggtggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacggtttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcattcagg ctgttaatca gtgcatcgaa
     4381 gtcaagagtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacactc gcttaacgct
     4501 ggtatttttt gccaggaaat cggcagctac gcgtagtgcg ccagtgcccc ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggagctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcag ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcagta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 tataaaaaaa acaggaccaa agtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gctcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt tttatgacac ctgccactgc cgtcaataag
     6181 ttctgtcaat aaaaatttac ggaactattg atgagagttt ggtgtcttta tgtgtctgca
     6241 ggcatctttc cattcaaact aacgtttcgc taccgtgaaa gtgctacaaa gataaagatt
     6301 tagtttcaaa aagaaaaaat atgtaaccaa aagtaaaaat taaggaactt tgtgaacacc
     6361 gtcatatttc catagaaacg tgatgatatt tacagcaatt tttatctatt tatatgattt
     6421 acttatattt aaattaacta aacggaaatt ttgtttctga tggaaacttt atcgacctgg
     6481 cacaaaatcc tctttcagac atccagaatg ccaaaaaata gtatgaaaat tgtgctatca
     6541 gctaagaaaa aactaatccg caataagaat gcgggttatt ttttgtgcgt aaatgttgca
     6601 atgtaatatt ccgtaacagg atgatcgtta tggcggggtt gcaaatagat tgcttgactt
     6661 aacaaacaga taacttgaca gaaaggataa aaaagagcca gcaggcgctg gctctttgag
     6721 gaagaaaatc agaagctggc gttgcgcgga gtacgtggga acggaatcac atcacgcacg
     6781 ttttgcacgc cagttacgta agcaatcagg cgttcaaaac caagaccgaa acctgaatgc
     6841 ggaacagtac cgtagcgacg cagatcgcga taccaccagt aatcttcttt attcaggccc
     6901 atttccagca tacgctcgtc cagaacgtcc agacgttctt cacgctggga gccaccaatg
     6961 atctcaccga tgcccggagc cagaacgtcc atagccgcaa cggttttacc gtcttcgttg
     7021 aggcgcatat agaacgcttt aatatctttc ggatagtttt taaccactac cggtgcttta
     7081 aagtgttctt ccgccagata acgctcatgc tcagaagaga gatcgacgcc ccagtaaacc
     7141 gggttttcaa acttcctgcc gcagttttcg agaatggtca ctgcgtcggt gtaatccacc
     7201 tgcgcgaaat cggcctcaat gaagcgttcc agacgtgaaa cggcatcttt atctacgcgt
     7261 tcagcgaaga atttcatgtc gtctgcgcgt tcttcgagaa ccgctttgaa gacatatttc
     7321 agcatggctt cagccagacc cgcgatatcg ttcaggttag caaacgccac ttccggctcc
     7381 agcatccaga attccgccag gtgacggctg gtgttggagt tttcagcacg gaaagtcggg
     7441 ccgaaggtat aaattttgga taatgcgcaa gcgtaggttt cgccgttcaa ctggccagat
     7501 acggtcagga aagactcttt accaaagaag tctttgtcga aatccacttt gccctgatcg
     7561 ttacgcggca ggttttccag atccagcgta gaaacgcgga acatttcgcc tgcaccttcg
     7621 gtatcagatg cggtaatcag tggcgttgaa acccagaaga atccctgctc gttaaagaag
     7681 cgatgcagcg cctgcgccag cgtatggcga acgcgcgcga cggcaccaat caggtttgtg
     7741 cgcggacgca ggtgagcgac ttcacgcaga tactcaatgc tgtggcgttt tgccgccatc
     7801 gggtaagtgt ctggatcttc aacccaacca gcaacttcaa ccttgctggc ctgaatttca
     7861 aattgttgcc cctggcccgg cgatgccacg actttacccg tcacaatgac cgagcagccg
     7921 gtggtcagac gcaggacgtc ttcattgtaa ttgggcagag aattattgat gacagcctgt
     7981 acaggatcaa agcaggaacc gtcataaacg gcgaggaagg agatgccagc ttttgaatct
     8041 cggcgggtac gtacccatcc gcgtacggtg acttcgctgt caacggctac gcggccctgg
     8101 agtacgtcgg ctacaggcac aacgctcata atattctctc tgttaatagt cggaaaaaat
     8161 aaacacttgt ccacccgtaa atgggggggg gatacctatg ttacctggca tctgcaatca
     8221 gacaagcaga attcgcaaat gcagcgaaag atttcggaat taaaggtaaa aaaagggagc
     8281 cgattagctc cctgatgata ttaactggct tttttaatat gcggaaggtc gaacgctttg
     8341 cgcagcgccc gaacaaacgc tttatcatgg cagatagttt tgccagggct gtcagaaagt
     8401 ttcgccaccg gtttaccgtt acactctacc aacttaatca caatattcag gggttttacc
     8461 tgggggatat cgcaggtcag gcgagtccca ataccaaaac ttaattgcac gcgggaagag
     8521 aagtggcggt atagctcaac cgctttgcgt aaatccagat tgtcagagaa aaccagcgtt
     8581 ttactctgtg gatcaattcc cagcttttca taatgtgcaa tggctttttc accccattca
     8641 accgggtcgc cagagtcatg acgcaggccc tgataccgac tagcgaactc gacaccgaaa
     8701 tcacgcagga aagcatccat agtgatgcag tcggttaatg caatgccaag ttggtcggga
     8761 tactcttcca gccaggcagc aagtgcagct cgctggctgt tggctagatc cgggctgatt
     8821 tgctgatgtg cctggaacca ttcgtgtgcc tgtgttccca tcggcgtgag ggaaagccga
     8881 cgcgccagat cgtagttgct ggtgcccacg aaccaggatt cctgttgcag acgcttaacg
     8941 atggtttctt gtacttcgcg agaaaaacgg cgacgggtgc caaaatccat cagatggaag
     9001 cgcgacatat caagaccggc ggttaacgcc gagaagtcga ctaatttgct ttccagcgtg
     9061 tcgagggctt gcgcaacgtc ggcctgcggt gagcgatagc gatgtaccat ttcactgata
     9121 accgccagca aaggaacttc ccagaggatg acttcacgcc acggaccgct taagcgaata
     9181 tccagcttgc cattatcgtt ggacacggtg acttgttccg ggttaaagcg gaactcgcgt
     9241 aaccagttaa gatagtcggc cttaaagaaa ggcagggcag agagccactg atattcatca
     9301 tcctgcaggc gcaggtgctg catcgcctga atctgttcac gaatagcatc ggcataaata
     9361 cccagcagat cgtcacctcg gcaacgaaac tccgccgcga catgcacatc gtaatagtga
     9421 tgaaacacgg cttgctgcat atgcaactta taagcatctg tatccagcaa cgagtgcaga
     9481 acaggagaag cgaattgtgt cataggtgcg cagtagcgtc ctcgtacagg agcgtttagt
     9541 acaataaaca tcttcaggaa ctgctggagt ataccttgtt tcgcaattta ttgaaccccg
     9601 atcacaccat atgccacctt tctggtcgat ggcattacgc ggtgtatgtt ataaaaatgt
     9661 agcaataaag gcgtttgtac ctgaaaagat gaagattctg catagcgcga tttacgcaac
     9721 aggaatagac tgaacaccag actctataaa agatgctaaa ggttatttat gactcaacag
     9781 ccacaagcca aataccgtca cgattatcgt gcgccagatt accagattac tgatattgac
     9841 ttgacctttg acctcgacgc gcaaaagacg gtcgttaccg cggtcagcca ggctgtccgt
     9901 catggtgcat cagatgctcc acttcgtctc aacggcgaag acctcaaact ggtttctgtt
     9961 catattaatg atgagccgtg gaccgcctgg aaagaagaag agggcgcact ggtcatcagt
    10021 aatttgccgg agcattttac gcttaagatc gttaatgaaa taagcccggc ggcgaatacc
    10081 gcgctggaag ggctttatca gtcaggcgat gcgctttgca cccagtgtga agccgaaggt
    10141 ttccgccata ttacgtatta tctcgaccgc ccggacgtgc tggcgcgttt taccaccaaa
    10201 attattgccg ataaaaccaa atatcccttc ctgctttcca acggtaaccg cgttgcgcaa
    10261 ggtgaactgg aaaacggacg ccattgggta cagtggcagg acccgttccc gaaaccgtgc
    10321 tacctgtttg cgctggtggc aggcgacttt gatgtactgc gcgacacctt taccacgcgt
    10381 tctggtcgcg aagtggcgct ggagctgtac gtcgatcgcg gcaaccttga tcgcgcgccg
    10441 tgggcgatga cctcgctgaa aaactcaatg aaatgggatg aagaacgctt cggcctggag
    10501 tatgacctcg acatctatat gatcgtcgcg gtggatttct tcaatatggg cgcaatggag
    10561 aataagggtc tgaatatctt taactccaaa tatgtgctgg cccgcaccga caccgccacc
    10621 gacaaagatt acctcgatat tgaacgcgtt atcggccatg aatatttcca taactggacc
    10681 ggtaaccgag tgacctgccg cgactggttc cagctcagcc tgaaagaagg tttaaccgtc
    10741 ttccgcgatc aggaattcag ctctgacctt ggttcccgcg cagttaaccg catcaataat
    10801 gtgcgcacca tgcgcggatt gcagtttgcg gaagacgcca gcccgatggc gcacccaatc
    10861 cgcccggaca tggtcattga gatgaacaac ttctacaccc tgaccgttta cgagaagggc
    10921 gcggaagtga tccgtatgat ccacaccctg ctgggcgaag aaaacttcca gaaagggatg
    10981 caactctatt tcgagcgtca tgatggtagc gcagcgacct gtgacgactt tgtgcaggcg
    11041 atggaagatg cgtcgaatgt tgatctctcc catttccgcc gttggtacag ccagtccggt
    11101 acgccgattg tgaccgtcaa agacgactac aatccggaaa ctgagcagta caccctgacc
    11161 atcagccagc gcacgccagc cacgccggat caggcagaaa aacagccgct gcatattccg
    11221 tttgccatcg aattgtatga caacgaaggc aaagtgatcc cgttgcagaa aggcggtcat
    11281 ccggtgaatt ccgtgctgaa cgtcacccag gcggaacaga cctttgtttt tgataatgtc
    11341 tacttccagc cggtgcctgc gctgctgtgc gaattctctg cgccagtgaa actggaatat
    11401 aagtggagtg atcagcaact gaccttcctg atgcgtcatg cgcgtaatga tttctcccgt
    11461 tgggatgcgg cgcaaagttt gctggcaacc tacatcaagc tgaacgtcgc ccgtcatcag
    11521 caagggcagc cgctgtctct gccggtacat gtggctgacg ctttccgcgc ggtgctgctc
    11581 gatgagaaga ttgatccggc gctggcggca gaaatcctga cgctgccttc tgtcaatgaa
    11641 atggctgaac tgttcgatat catcgacccg attgctattg ccgaagtacg cgaagcactc
    11701 actcgtactc tggcgactga actggcggat gagctgctgg ctatttacaa cgcgaattac
    11761 cagagcgagt accgtgttga gcatgaagat attgcaaaac gcactctgcg aaatgcctgc
    11821 ctgcgcttcc ttgcttttgg tgaaacgcat ctggctgacg tgctggtgag caagcaatac
    11881 catgaagcaa acaatatgac cgatgcgctg gcagcgcttt ctgcggcggt tgctgcacag
    11941 ctgccttgcc gtgacgcgct gatgcaggag tacgacgata agtggcatca ggacggtctg
    12001 gtgatggata aatggtttat cctgcaagcc accagcccgg cggcgaatgt gctggagacg
    12061 gtgcgtggtc tgttgcagca tcgctcattt accatgagca acccgaaccg tattcgttcg
    12121 ttgattggtg cgtttgcggg cagcaacccg gcagcgttcc atgccgaaga tggcagcggt
    12181 taccagttcc tggaggaaat gcttaccgac ctcaacagcc gtaacccgca ggtagcttca
    12241 cgtctgattg aaccgctgat tcgcctgaaa cgttatgatg ccaaacgtca ggagaaaatg
    12301 cgcgcggcgc tggaacagtt gaaagggctg gaaaatctct ctggcgatct gtacgagaag
    12361 atcaccaaag cactggcttg atacttttga gccaaaatgc ctgatgcgac gcttgacgcg
    12421 tcttatcagg cctacaactc cgttgaaatt gctggttttg taggctggat aaggcgttca
    12481 cgccgcatcc ggcagccgtg cccaactgcc tgatgcgatg cttgacgtgt cttatcaggc
    12541 ctacaagtcc gttgaaaatg ctggttttgt gcgccacatc cggcatatta accatgacgc
    12601 cgaatcagtt gttcagaatg cccacgccgc attacccgtt gcaatacttc cgcttccagc
    12661 tccgctaatc gcaccgaccc taatcgacgc ggacggggga tatccactgt caaatccaga
    12721 ccaatttttc cctcttcaat taacaacacc cggtcagcca ttgctaccgc ttcgctcaca
    12781 tcatgcgtca ccaacagtac ggtaaagccg tgctgctgcc aaagtgacac aatcaaatcc
    12841 tgcatctcga gtcgcgttaa ggcgtccagt gccccgagcg gttcatcaag caacaataaa
    12901 cccggtcgat gaatcaacgc tcttgccagc gccacacgct gtttctgccc gccagaaagt
    12961 gcggcaggcc attccccggc gcgattctcc agtcctactg cagccaacgc ccgacgcgcg
    13021 gcatcgcgcc actggccttt aaggcctaac ccaacgttat caatcaccga tttccacggc
    13081 agcagacgcg catcctgaaa catcattcgc gtgtcttcct gaatctcagc cagcggtgtg
    13141 gtgcccgcta acacatcgcc tgcggttggc gtttccagac ctgccagcag gcgcagcagg
    13201 gtacttttgc caccaccgct gcggcccacc accgccacaa actgacctgc cggaatatgt
    13261 aaatccagtt ggttcaggac gatattttcc gcgtaatgtt tgcttactgc attgagcaac
    13321 aatggtgtgc cctggttcag acgagcagta ttcataccgt ggcctccttc aaatgataag
    13381 ccgggttcca gcgcaaccag aggcgctcta acagctgcgc gctgacgtca gcaagtttgc
    13441 cgagcagggc gtaaagaata atagcgacta ccaccacgtc cgtttgcaaa aactcccgcg
    13501 cattcatcgc cagataacca atgcctgaat tggcagaaat ggtttcggca acaatcagcg
    13561 tcagccacat caggcccaac gcaaaacgca cgccgaccat aattgagggc agggcaccag
    13621 gcaggatcac atggataaac agcggtatgc cggataatcc atagctacgc gccatttcca
    13681 ccagaccgcg atcgatatta cggatgccat gccaggtgtt gatataaatg gggaatagcg
    13741 tgccgagcgc caccagaaag atttttgcgg attcatcaat gccaaaccac aaaatcacca
    13801 gcgggatcag cgccagatgc ggtacgttgc gcaacatctg aattgaggta tccagcagcc
    13861 gttctcccca gcgtgacagc ccgctaatca gtcccagaat caagcccagc gatccgccaa
    13921 ttgaaaagcc aatcagcgcc cgccaggaac tgatcgccag atgctgccac aattcgccgc
    13981 tggcggagag cgtccagaac gccgtcacca ctccttccgg tgaaggcaaa atacgcgtcg
    14041 acagccagcc aaccgaggag gccagttgcc acaccgccac gatgcccacc ggtaaaaacc
    14101 agggggcaac gcgcaataac cacttcttca ctggcgttgc catcatcgtg ttccttagct
    14161 ttgcgcgact ttacggggga taaaatcatt cgccaccgct tcgccttgcg gattcagcgg
    14221 ctgcggctgg ggaatttccg gaatggcgac atccagatgt gggaacagca actcgccaac
    14281 ccgatacgct tcttccagat gcgggtagcc cgaaagcaca aagctgtcga tgccaagcgc
    14341 ggcgtattcg ttgattcgcg cggcgaccgt aggaccatcg cccaccagcg ccgttccggc
    14401 accgccgcgc actaagccaa cgcccgccca taaattgggg ctgatctcca gattgtcgcg
    14461 cttgccgtta tgtagcgccg ccattcgctg ttgtcctacg gaatccgtcc gggcgaatgc
    14521 ggcctgtgct ttggcgatag tttcatcatc aagatgcgag attaaccgct cggcggcctg
    14581 ccacgcttcg tcgttagttt cacgaacaat cacatgcaga cgaataccga aacgaatttt
    14641 gcgtccatgc gcggcagctt tcgcccgcac ttgttcgatc ttctctttta ccagctctgg
    14701 cggttcgccc caggtgaggt agagatcaac ttgttctgcc gccagctcct gggcgacatc
    14761 tgacgatccg ccaaagtaaa gcggaggata cggctgttga atcggcggga agagcagttt
    14821 tgctccccgc acatgaatat gtttaccgtt gaaatcgacg ctttccccca gcaataagcg
    14881 ccccgccaga cctgggtaaa ttccgccgag gcttcgtagc gctcgctatg atcaaggaac
    14941 acgccgtcgc ctgccaactc ttgtggatcg ctgcctgtga ccagattaaa caacgcacga
    15001 ccatttgaga gacggtcaag cgtggcggcc tggcgagcgg caacggtagg tgaggttacg
    15061 ctgggacgca gggcgacaag aaacttcagc cgctgcgtca ccgggatcat cgacgccgcc
    15121 accagccacg catcttcaca cgatcgcccc gttgggatca gcacaccggt atagcccaga
    15181 cgatccgccg cttgcgcaat ttgttgcaga tagccgtgat caaccgggcg tgaaccttct
    15241 tccgttccaa gataatgccc gtcaccatgg gtcggtaaaa accagaacat attcagactc
    15301 ataattgttt tccttccagg tgagtgggct gccagatgcg ctggcgaata tcaacttttt
    15361 tcgacaccag acggttttca taaaacagat ctgccgtttg ctgctgtaag gcggcaactt
    15421 cagcgctcac cggtttgatg gtggtgggag gacgatgatc cagatacgag gcaatcaccg
    15481 gtgccggtaa gcccatcgtt tttgccagta aagcgatgct ttgctcgcgc tggctgcggg
    15541 ttaacgcatc ggcctcacta aaggttgcca gtacgccctg aataaaagcg ccgttttttt
    15601 ctgcataggg gcgagctgcc agataaaacg atccagtttg attgagatcg gtgccgtctt
    15661 tcagcacccg cacgccgccc tgtaataacg cagcggagta gtagggatcc cagatagccc
    15721 aggcgtcaac gttaccttgc tggaacgcgg cgcgggcatc agctggcgtc agataagtgg
    15781 gctggatgtc agtaaactta agtccggcct gacgcagtgc acgcagtaaa aggttgtgtg
    15841 aactggaacc tttctgaaag gcaactttgt gacctttaag atcggctacg gttttgatcg
    15901 ggctgttttc tgccaccaga atcacttcgg ctttgggctt cggtggctcg acgcccacat
    15961 atatcaaatc agccccggca gcctaggcaa agattggcgg aatatccccg gtactgccga
    16021 gatcaatact gccaacgttt aacgcttcca acatttgcgg acccgcgggg aactccaccc
    16081 aggagatttt tgattgcgga tagcgttttt ccagtaactg gtggcttttt gccaatacca
    16141 taccaatact gcctttctga tagcctatac gtaacgcttc aggcgaggat tctgcagcaa
    16201 ccgcaaaagt agagacgcta agcaatcccg ccagcgccag tttaatgatg ttacgcatgg
    16261 acattacctc gcagagacag aaggtcagga acctgaacat cgcggcggtg caatgcctgc
    16321 cagaaagttt ctagcgcggt atcaagacgg gtttgcagat ttggcgtgaa ctggggtttg
    16381 tgatggtaat caataacttg tgagtcatcg gcaaacacgc cgtgcaggag ctcctgggct
    16441 ttcatcgcac ttaaaactgg cttaagggca taatcgaccg ccagcagatg agccaccgta
    16501 c
//
LOCUS       DAJVQG010000091        16282 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJVQG010000091 DAJVQG010000000
VERSION     DAJVQG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16282
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.091"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..567
                     /locus_tag="OQM83_002613"
     CDS             <1..567
                     /locus_tag="OQM83_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCS1520846.1"
                     /translation="NNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDNDGDG
                     TFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENSYYDNVEQS
                     WIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKESYSS
                     GGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            570..1733
                     /locus_tag="OQM83_002614"
     CDS             570..1733
                     /locus_tag="OQM83_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCS1520847.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1817..3442
                     /locus_tag="OQM83_002615"
     CDS             1817..3442
                     /locus_tag="OQM83_002615"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCS1520848.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(3559..3834)
                     /gene="yjfN"
                     /locus_tag="OQM83_002616"
     CDS             complement(3559..3834)
                     /gene="yjfN"
                     /locus_tag="OQM83_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCS1520849.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3983..4312)
                     /gene="bsmA"
                     /locus_tag="OQM83_002617"
     CDS             complement(3983..4312)
                     /gene="bsmA"
                     /locus_tag="OQM83_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCS1520850.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4494..5243
                     /gene="yjfP"
                     /locus_tag="OQM83_002618"
     CDS             4494..5243
                     /gene="yjfP"
                     /locus_tag="OQM83_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1520851.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5240..5995)
                     /gene="ulaR"
                     /locus_tag="OQM83_002619"
     CDS             complement(5240..5995)
                     /gene="ulaR"
                     /locus_tag="OQM83_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCS1520852.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6103..7167)
                     /gene="ulaG"
                     /locus_tag="OQM83_002620"
     CDS             complement(6103..7167)
                     /gene="ulaG"
                     /locus_tag="OQM83_002620"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCS1520853.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7522..8920
                     /gene="ulaA"
                     /locus_tag="OQM83_002621"
                     /pseudo
     CDS             7522..8920
                     /gene="ulaA"
                     /locus_tag="OQM83_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            8936..9241
                     /gene="ulaB"
                     /locus_tag="OQM83_002622"
     CDS             8936..9241
                     /gene="ulaB"
                     /locus_tag="OQM83_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCS1520854.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9251..9715
                     /gene="ulaC"
                     /locus_tag="OQM83_002623"
     CDS             9251..9715
                     /gene="ulaC"
                     /locus_tag="OQM83_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCS1520855.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            9729..10379
                     /gene="ulaD"
                     /locus_tag="OQM83_002624"
     CDS             9729..10379
                     /gene="ulaD"
                     /locus_tag="OQM83_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCS1520856.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10389..11243
                     /gene="ulaE"
                     /locus_tag="OQM83_002625"
     CDS             10389..11243
                     /gene="ulaE"
                     /locus_tag="OQM83_002625"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCS1520857.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            11243..11929
                     /gene="ulaF"
                     /locus_tag="OQM83_002626"
     CDS             11243..11929
                     /gene="ulaF"
                     /locus_tag="OQM83_002626"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCS1520858.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATYLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            12026..12577
                     /locus_tag="OQM83_002627"
     CDS             12026..12577
                     /locus_tag="OQM83_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HCS1520859.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(12652..12967)
                     /locus_tag="OQM83_002628"
                     /pseudo
     CDS             complement(12652..12967)
                     /locus_tag="OQM83_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            13294..13689
                     /gene="rpsF"
                     /locus_tag="OQM83_002629"
     CDS             13294..13689
                     /gene="rpsF"
                     /locus_tag="OQM83_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCS1520860.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13696..14010
                     /gene="priB"
                     /locus_tag="OQM83_002630"
     CDS             13696..14010
                     /gene="priB"
                     /locus_tag="OQM83_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCS1520861.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            14015..14242
                     /gene="rpsR"
                     /locus_tag="OQM83_002631"
     CDS             14015..14242
                     /gene="rpsR"
                     /locus_tag="OQM83_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCS1520862.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14284..14733
                     /gene="rplI"
                     /locus_tag="OQM83_002632"
     CDS             14284..14733
                     /gene="rplI"
                     /locus_tag="OQM83_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCS1520863.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14845..15816)
                     /locus_tag="OQM83_002633"
     CDS             complement(14845..15816)
                     /locus_tag="OQM83_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCS1520864.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(15869..>16282)
                     /locus_tag="OQM83_002634"
     CDS             complement(15869..>16282)
                     /locus_tag="OQM83_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCS1520865.1"
                     /translation="WAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIGTFMDMT
                     PAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVSKLPINK
                     IIKPMLPFYAVMVLVLAMVTYIPQISMALPKALGY"
BASE COUNT         3823 a         3979 c         4329 g         4151 t
ORIGIN      
        1 aataatcctt ttgaaccgcg tcgcaatcgc tacgcacaaa aatacttaac gttggcgcta
       61 atgggcggtg ccgctttttt cgtattgaaa ggttgtggcg atagtagcga tgtcgataac
      121 gacggcgacg gaacgtttta cgcgacagtg caggattgta ttgatgacgg taataatgcc
      181 gacatttgcg cgcgtggctg gaacaacgcc aaaacggcat tttatgccga tgttccgaag
      241 aatatgactc agcagaactg tcagtctaag tacgaaaatt cctactacga caatgttgaa
      301 cagagttgga tcccggtcgt ttctggattt ttgttaagcc gggttattcg taaagatcgc
      361 gatgagccgt tcgtctataa cagcggcgga tcctcatttg cttcccgccc cgtctggcgc
      421 aacacttccg gtgattactc ctggcgctcc ggttctggca aaaaagagtc ttactcttca
      481 ggcggcttta ccaccaaaaa agcgtcaacc gtttcgcgcg gcggctatgg tcgttcttcc
      541 agcgcccgtg ggcattgggg aggctaatca tgctgagaca caacgttcct gtgcggcggg
      601 atctggacca aattgccgct gacaacggtt tcgactttca tatcatcgac aatgaaatct
      661 attgggacga gagtcgggct taccgtttta ctctgcgcca gattgaagag cagatcgaaa
      721 aaccaactgc ggaactgcat cagatgtgcc ttgaggtggt ggatcgcgcg gttaaagacg
      781 aagagatcct gacgcaactg gcgatcccgc cgttgtactg ggatgtgatc gccgaaagct
      841 ggcacgccag cgatccttcg ctgtatggcc ggatggattt tgcctggtgt ggcaatgcgc
      901 cggtgaagct gctggagtac aacgccgata cgccgacttc gttgtatgag tcggcttatt
      961 tccagtggtt gtggctggag gatgcccggc gcagcggcat tattccgcgt gatgccgatc
     1021 agtacaatgc cattcaggaa cgcctgattt cacgctttag cgagctttat agccgggagc
     1081 cgttttattt ctgctgctgt caggacaccg atgaagacag gactaccgtg ctgtacttgc
     1141 aggactgcgc ccagcaggca gggcaggagt cgcggtttat ctacattgaa gatctcggtt
     1201 tgggcgtcgg cggtgtactg accgatcttg atgataatgt catccagcgt gcatttaagc
     1261 tgtatccgct ggagtggatg atgcgtgacg ataacggtcc gctgctgtgc aagcgtcgcg
     1321 agcaatgggt ggagccgtta tggaaaagta tcttgagtaa taaagggcta atgccgctgc
     1381 tttggcgctt cttccctggt catcctaacc ttcttgcatc ctggtttgag ggtgaaaaat
     1441 cgcagattgc cgctggcgaa agctatgtgc gtaaaccgat ctactcgcgc gaaggcggaa
     1501 acgtcaccat tttcgacggt cagaataacg tcgttgacca cgctgatggt gattacgccg
     1561 atgaaccgat gatctaccag gcgtttcaac ctctgccgcg atttggcgat agctacacac
     1621 tcatcgatag ctggattgtc gatgatgaag cgtgcggaat ggggatccgt gaagataaca
     1681 cactgatcac caaagacacc tcacgtttcg ttccgcatta cattgctgga taagaatgtt
     1741 ttagcaatct ctttctgtca tgaatccatg gcagtggcca tactaatggt gactgccatt
     1801 gatggaggga gacacagtgc actggcaaac tcacaccgtt tttaatcaac ctataccatt
     1861 aaataacagc aacttatacc tgtctgatgg cgcgctttgc gaagcggtaa cgcgtgaagg
     1921 tgctggctgg gatagcgatt ttctagccag tattggtcag cagttaggaa cggctgaatc
     1981 ccttgaactg gggcggctgg cgaatgtaaa cccgcctgaa ctgttgcgct acgatgccca
     2041 ggggcggcgt ctggacgatg ttcgtttcca tcccgcatgg cacctgctga tgcaggtgtt
     2101 atgtaccaat cgggtgcaca atcttgcctg ggaagaagac gctcgctccg gcgcatttgt
     2161 ggcgcgcgcg gcgcgtttta tgttacatgc gcaggttgag gctgggtcgt tatgtccaat
     2221 aacgatgacc tttgccgcca cgccattgtt gttacagatg ttacccacgc cgtttcagga
     2281 ctggaccacg ccgctgctga gcgatcgcta cgattctcac ttattgccag gtgggcaaaa
     2341 acgcggtttg ttgattggca tgggaatgac ggaaaagcag ggtggttccg atgtcatgag
     2401 caacaccacc cgtgcagagc gtttggaaga tggctcttat cggctggtgg ggcataaatg
     2461 gtttttctcg gttccgcaaa gcgatgcgca tctggtgctg gcgcagacca cgggcggtct
     2521 gtcctgtttt tttgtgccgc gctttttgcc tgacgggcaa cgcaacgcga ttcgcctcga
     2581 acggctgaaa gataagctgg gtaatcgctc taacgccagt tgtgaagtgg agtttcagga
     2641 tgccattggc tggttattgg ggcaggaagg ggaaggaatt cgtctgatcc tgaaaatggg
     2701 ggggatgacg cgttttgatt gcgccctggg tagccatgcc atgatgcgcc gtgcgttttc
     2761 gctggcgatt tatcatgcac atcaacgcca tgtttttggt aatccattga tccaacagcc
     2821 ccttatgcgt catgtcttaa gtcgcatggc acttcagctt gaagggcaaa cggcgttgct
     2881 gtttcgtctt gcgcgagcgt gggaccggcg cgccgatgcc aaagaagccc tgtgggcgcg
     2941 tttatttacg cctgcggcaa aatttgtgat ctgcaaacga ggtatgccgt ttgtggccga
     3001 agcgatggag gtgctgggcg gcattggtta ttgcgaggag agcgagctgc cgcggcttta
     3061 ccgggagatg ccggtaaaca gtatttggga aggttccggc aatattatgt gcctggatgt
     3121 gttgcgcgtt ctcaataagc aagcgggcgt atacgactta ttgtcggaag catttgtgga
     3181 agtgaaaggg caggatcgct attttgatcg cgcggttcgt cgtttacagc agcagctgcg
     3241 taagccagct gaagaactgg ggcgagagat tactcatcag ctattcctgc tgggctgcgg
     3301 tgcgcaaatg ttgaaatatg cttctccgcc aatggcgcag gcgtggtgtc aggtgatgtt
     3361 agatacgcgc ggcggcgtac ggttgtcaga gcagatccag aatgatttat tgctgcgggc
     3421 gacgggggga gtgtgtttgt aaacgtatac gactgatgcg acgctggttt cgattaactc
     3481 aatgaaatat gtgaaaattg taggccggac aaggcgctcg cgccgcatcc ggcattgttc
     3541 atttgctgta gatgacactc atgcatacag tatcgcctgt acgcgccagt tatcaggctg
     3601 ctggtcttcg tacatggtaa taatgcggta ccatgacgca ccttgttcat cggcctttaa
     3661 cgccacgata cgttccacgt cctgcggatc ccctgaaata ttgctaactg agatctgacc
     3721 tatttcattc aggcccgtca cgcagtcagc actggcgaat tctgcagact gtgccgtggc
     3781 gctcgcagga taagtcacta attgcagcga gggtgaggca agtaattgtt tcatcgtcag
     3841 ctccattttt cgtttcccca catgttgcag ggcattccat cgcggtaata aacgcccagg
     3901 cttccagccg gtaatttatt gtgcacaggt aaacgcatag ggacgcaaag caatgtaaag
     3961 ctgactatgt aagttgtctg atttatttac ggtacaaaat ggcttgtgaa taccactgtc
     4021 ctgtcacgat ggtttcgtcc accatgacga caacgtaata atcagctttt gcagcgacag
     4081 cttttgctct gatttctgct aatgcgtcat ccggagaacc ccgaaccatc gtgcttacgc
     4141 tacctattcg ctgtaaccct tgcgtctggt cgcggcgaat ctcttgcgga tggtccgtta
     4201 ctggcggtgc tggctgtggc gtaccttgca gtgcgctaca ggcacttaac atcaacacca
     4261 ataataaact ggcaaaccgg taaataacgc tattacgttt cctgctaacc atagtgtagt
     4321 gccttattaa tttaactttg ggggaatgtt cccgaattgt tacctggtac gcgattttcg
     4381 aatgaaaacg tgacgaaagc gtaacccaca tctcaacatt atgaactaat accgccagca
     4441 gaaacttgtg ctaaggattt cttcatgtca taccagacaa aaaggagaga cagatgattg
     4501 aaatagaatc acgcgagctg gcagatattc ccgttcttca tgcttatcct gtcgggcaaa
     4561 aagatacccc gttaccgtgc gtaatttttt atcacggctt tacttcatcc agcctggtgt
     4621 atagctattt tgccgttgcg ctggcgcagg ctggtttgcg ggtgatcatg ccggatgcgc
     4681 ccgatcacgg tagccgtttt agtggcgacg aagcgcggcg gttaaatcaa ttctggcaaa
     4741 tcttgctaca aagtatgcag gaattcacta ctttacgtgc ggcaatagcc gaagaaaact
     4801 ggctgcttga tgaccgtctg gcagtcggtg gcgcgtcgat gggcgcgatg acggcactgg
     4861 gaattaccgc tcgccacccc acggtgaaat gtaccgccag catgatggga tcgggctatt
     4921 ttacatcgct cgcccgttca ctgtttccac cgctgatacc tgaaacggca gcacagcaga
     4981 atgaattcaa taacattatc gcgccgctgg cagagtggga agcgacaaac cacctggaac
     5041 aacttgggga cagacctcta ctgctgtggc atggcctcga cgatgacgtt gtgcctgccg
     5101 acgaatcact acgtttgcag caggccttaa gcgagacggg acgggataaa ctgctaacct
     5161 gttcatggca gccaggcgtg cgtcaccgca ttacgcctga ggcgttagat gctgccgtga
     5221 catttttccg ccagcatctt taaacacgca gaatgctgac cccttgcgct tccagttgtt
     5281 gcaggatttc cgggttagca tttttgccgg tgataagcat atcgatttga tcggcacggc
     5341 taaaaagcat tcccgcgcgt tcgccaatct tactgctatc aaccagtacc accagtttcc
     5401 ctaccacgct cagcatcttc tgctctgcca ttgctgtcag catatcggtt ttatacaacc
     5461 cttctgcggt cagccctttt ccgctggtaa acatccagtg cccggcatag agactgtttt
     5521 cgctgccctg cgggcttaaa gtgatggact gacttttgtt gtactgtcca cccataatga
     5581 tcacgctgtc atgttcttga tcgatcaggt aatttgccag cggtagataa ttagtgatga
     5641 tttgcactgg cttgccacac atttcccgcc caagcagaaa cgcggtggag ccgcagttga
     5701 tgactacgct ttcgcccgga ttaaccagct gcgaggccgc tttagcaata cgtacttttt
     5761 catcgtgatt ctgcgcctga tgcagattca tcggcgtcca gcgcgggcgc tgttgggtaa
     5821 tagcttctgc gccattgcgc acctttttca gtttgccgcg ttcgtcaagt ttattgatat
     5881 cgcgtcgcgc agtggcaggc gaaattccca gacgctcaac gactttctca acggtcacaa
     5941 agcccaattg tgcgagcatt tccaggagga tttgatgtct ttgtgcttca gtcatgagct
     6001 attccgataa aatttgattt ttttagatga tatttgaaat agctaggaaa tactacgccg
     6061 gatagcgcga acttctccac gctatccggt ataaacgaca gattacagga atgacttgaa
     6121 cggcagatcc ggttcaatag tgaagcaatc atcgaaaccg cgcggatagt ggtactcgaa
     6181 gttgtcttta tccagcggcc aggtaaattt gccgccaacc tgccagataa acggcttgaa
     6241 gccatacttc agacgatctt ttttcatctc ccacagcacg cggatctctt gcggatcggc
     6301 ctggaagttt gaccagatgt cgtggtggaa cgggataacc actttcgcat tcagggcttc
     6361 acccatacgc agcatatcgg cgctggtcat tttgtcggta ataccgcgcg ggttctcacc
     6421 atacgatcct aacgccacat cgatctgatg ttcgttaccg tgcttcgcat aatagttaga
     6481 gtagtgggaa tcgccactgt gatataggga accgccaggg gttttgaaca ggtagtttac
     6541 tgcgcgatcg tccatgccat ctggcagtac gccagccgct ttttgatcgg caggcagggt
     6601 gatcagtgca gtacggtcga aagcgtcaag cgcatgaatt tcaatgtctt tcacttttac
     6661 aacatcgccc ggtttgacca cgatgcaacg ctctttcggt acgccccagc caatccacaa
     6721 atcaacgcag gttttcggtc caatgaatgg tacgtcatca gcacagttct gcatcacggc
     6781 agcagcgacg ttaacgtcga tatgatcgtt gtgatcgtga gtcgccagta ccgcgtcgat
     6841 ctggcgaatc gcaaacggat caagaacaaa cggggtggtg cgtaggtttg gctgcagttt
     6901 tttcacgcca gccatgcgct gcatctggtg accctgtttc attaacgggt taccgtgact
     6961 ttgtttgcca gtgccgcacc agaaatcaac gcaaacgttg gtgccacctt ctgatttcaa
     7021 ccagatcccg gtgcagccta gccaccacat cgcaaatgtg ccaggggcga cctgctcttg
     7081 ttcaatttct tcattcagcc agctacccca ctccgggaaa gtgctcagga tccaggattc
     7141 tcgggtgata cttttcactt tactcatcgc catttacctt catgatagtt caattcgaat
     7201 caatatgtga ttggttttga ttaatcctga cactattttt tcaggaaggc aatgaccatt
     7261 ttttgacttt ttgccaggga agttgttgtt gattttgagt gtggaaagat ttaatggaat
     7321 gtgtagttaa attaactaaa tgaatttaaa taaataattg tttcgttgtg tgaatcccac
     7381 tctatccgtg tggaattatt tgcgggtcgc gtcacattta atcataaata atcttgttgt
     7441 gattactttt gaaaattaga gtgagtgcac aacattccgg gtgtgcggaa tacccggtta
     7501 cctcttcttc aggagatcgt tatggagatc ctctacaaca tctttaccgt gttttttaac
     7561 caggtcatga ccaatgcccc gttgttgctg ggtattgtga cctgtctggg ctacatccta
     7621 ctgcgcaaaa gtgtcagcgt tattattaaa ggcacgatta aaaccataat tggtttcatg
     7681 ttgttgcagg cagggtccgg catcctcacc agcaccttca aaccggtggt ggcgaaaatg
     7741 tccgaagtct acggcattaa acggcgcaat ttccgatact tacgcgtcga tgatggcaac
     7801 catcgaccgc atgggcgatg cctatagctg ggtaggttac gcggtgttgt tagcgctggc
     7861 gctgaacatc tgttacgtgc tgctgcgtcg cattaccggt attcgtacca tcatgctgac
     7921 tggtcatatc atgttccagc aggcggggct gattgccgtt acgttgttta ttttcggcta
     7981 ctccatgtgg acaaccatta tctgcacggc gattctggtt tcgctctact ggggcattac
     8041 ctccaacatg atgtacaagc cgactcagga agtgacggac ggctgcggtt tctccatcgg
     8101 tcaccagcaa cagtttgcat catggactgc ctataaagtt gcgccgttcc tcggcaaaaa
     8161 agaggaaagc gttgaagacc tcaaactgcc aggctggctg aatattttcc acgacaacat
     8221 cgtctccacg gcgattgtca tgaccatctt ctttggtgcc attctgctct ccttcggtat
     8281 cgacaccgtg caggcgatgg caggcaaagt gcactggacg gtctatatcc tgcaaaccgg
     8341 tttttccttc gcggtggcga tcttcatcat cactcagggt gtgcgcatgt ttgtggcgga
     8401 actctctgaa gcgtttaacg gtatctccca gcgcctgatc cctggtgcgg ttctggcgat
     8461 tgactgtgcg gctatttata gcttcgcgcc gaacgccgtg gtctggggct ttatgtgggg
     8521 caccatcggt cagctgattg cggttggcat cctggtcgcc tgcggttcct cgagcctgat
     8581 tatcccaggc tttatcccga tgttcttctc caacgccacc atcggcgtgt tcgctaacca
     8641 cttcggcggc tggcgtgcgg cgctgaagat ttgtctggtg atggggatga tcgaaatctt
     8701 tggttgcgtc tgggcggtga aacttaccgg tatgagtgcc tggatgggga tggcggactg
     8761 gtcgattctg gcaccgccga tgatgcaggg cttcttctcc atcggtatcg cctttatggc
     8821 cgtcatcatt gtaattgcac tggcttatat gttcttcgct ggccgcgcac tgcgcgcaga
     8881 agaagatgca gaaaaacaac tggcagaaca gtctgcttaa taaggagttt tgattatgac
     8941 cgtacgtatt ctggctgtgt gtggcaacgg acaaggcagt tccatgatca tgaggatgaa
     9001 agtggaccag tttttaaccc aatcaaacat tgaccacacg gtaaatagct gcgcggttgg
     9061 cgaatacaaa agcgagttga gcggcgcgga tatcatcatc gcttctacgc acattgcggg
     9121 cgaaatcacc gtgaccggca acaaatacgt ggttggcgtg cgcaacatgc tctctcctgc
     9181 cgactttggc ccgaaactgc tggaagtgat caaagcgcat ttcccgcagg atgtgaagta
     9241 aggacgcgcc atgaaattac gtgattcgct ggcggagaat aaatccatcc gcctgcaggc
     9301 tgaagctgag acatggcagg acgcagtaaa aatcggcgtt gacctgctgg tggcggcgga
     9361 tgttgtcgag ccgcgttact accaggcgat tctggatgcg gttgaacagc acggtccgta
     9421 ctttgtgctc gcaccaggcc tggcaatgcc gcacggtcgc ccggaagagg gcgttaagaa
     9481 aaccggtttt gcgctggtga cgctgaaaaa gccgctggag ttcaaccacg aagataacga
     9541 cccggtggat atcctcatca ccatggcggc ggtcgatgcc aacacgcacc aggaagtggg
     9601 catcatgcag atcgtcaacc tgtttgaaga tgaagaaaat ttcgaccgtt tacgtgcctg
     9661 ccgtaccgag caggaagtac tggatctcat tgaccgcacc aacgcggcag cttaagaagg
     9721 aattgaagat gtcattaccg atgttgcaag tcgcgctgga caaccagact atggatagcg
     9781 cctacgaaac cactcgcctg attgccgaag aagtcgacat tatcgaagtg ggtaccattc
     9841 tgtgcgtggg cgaaggcgtg cgtgcggttc gcgatctgaa agcgctctac ccgcacaaaa
     9901 tcgtgctggc agacgccaaa attgccgatg cgggcaaaat tctttctcgt atgtgcttcg
     9961 aagccaacgc tgactgggtg accgtgattt gttgtgcgga tatcaacacc gccaaaggtg
    10021 cgctggacgt agcaaaagag tttaacggcg acgtgcagat cgaactgacc ggttactgga
    10081 cctgggaaca ggcgcaacag tggcgcgacg caggtattca gcaggtggtt tatcaccgca
    10141 gccgtgacgc gcaggccgct ggcgtggcgt ggggtgaagc cgatatcact gcgatcaaac
    10201 gtctttccga tatgggcttc aaagtcaccg tcaccggtgg cctggcgctg gaagatctgc
    10261 cgctgttcaa aggcattcca attcatgtgt ttatcgccgg tcgcagcatc cgtgatgccg
    10321 cttctccggt tgaagccgcg cgtcagttca aacgttccat cgccgaactg tggggctaag
    10381 gagcggatat gttgtccaaa caaatcccgc ttggcatcta tgaaaaagcg ctccccgccg
    10441 gggagtgctg gctggaacgc ctgcgactgg caaaaacgtt gggcttcgat tttgtcgaga
    10501 tgtcggtaga tgaaactgac gagcgcctgt cgcgcctcga ctggagccgc gagcagcgtc
    10561 tggcgctggt gaatgcgatt gttgaaaccg gcgtgcgtgt gccgtccatg tgcctttctg
    10621 cccatcgtcg tttcccgttg ggcagcgaag atgacgcggt gcgggcgcag gggctggaga
    10681 ttatgcgtaa agccatccag ttcgcccagg acgttggcat tcgcgtgatc cagctggcgg
    10741 gctatgacgt ttactatcag gaagccaata acgaaacgcg tcgtcgtttc cgtgacggcc
    10801 tgaaagagag cgttgagatg gcgagccgcg cgcaggtgac gctggcgatg gagatcatgg
    10861 attatccgtt gatgaactcc atcagcaagg cgctgggtta cgcgcactat ctcaacaacc
    10921 cgtggttcca gctctacccg gatatcggca acctgtcggc gtgggacaac gatgtgcaga
    10981 tggagttgca ggccggaatc gggcatatcg tcgcggtgca tgtgaaagac accaaaccgg
    11041 gcgtcttcaa aaacgtgcca tttggcgaag gtgtggtgga tttcgaacgt tgcttcgaaa
    11101 cgctcaaaca gagtggctat tgcgggccgt acctgattga gatgtggagc gaaacggcgg
    11161 aagacccggc ggcagaagtg gtgaaagcgc gtgattgggt gaaagcgcgc atggccaaag
    11221 caggcacggt ggaggcggca taatgcaaaa gctaaaacag caggtgtttg aagccaacat
    11281 ggatctgccg cgctatgggc tggtgacctt tacctggggc aacgtcagcg ctatcgaccg
    11341 cgaacgcggg ctggtggtga tcaagcccag cggcgttgcc tacgaaacca tgaaagcaga
    11401 cgatatggtg gtggtcgata tgagcggcaa ggtggtggaa ggggagtatc gcccgtcttc
    11461 cgacaccgcg acgtatctcg aactctaccg tcgttacccg tcgcttggcg gcattgtcca
    11521 tacccactcc actcatgcta ctgcatgggc gcaggcgggg ctggcgatcc cggcgttagg
    11581 cactacgcac gccgactact tctttggcga catcccgtgt acgcgcggat taagcgaaga
    11641 agaggtgcag ggcgagtatg aactgaacac cggcaaagtg attatcgaaa cgctgggtga
    11701 cgccgagccg ctgcatacgc cggggattgt ggtgtatcag cacggtccgt tcgcctgggg
    11761 gaaagatgcc cacgacgcgg tgcataacgc ggtggtgatg gaagaagtgg cgaaaatggc
    11821 gtggattgcg cgcagtatta acccacaact gaatcacatc gacagcttcc tcatgaataa
    11881 acacttcatg cgtaagcacg gtcctaacgc ctattacggg cagaagtaag cagtgcgccc
    11941 cggaattatt acctcctgcg ggatgtaatt ggtctgattc cggggccgct tcagttaaaa
    12001 aatcgtataa acaacaggtg tttttatggc tatcaatgga ttgtttgtca gaacggatcc
    12061 gcaacgtcgc cgctatggcg ttgcgttact tgtgggtgta ctttcaggaa ttatttcagc
    12121 atttgttaaa tggggagcgg aagtcccctt gccaccgcgc acattttccg gtggtcgcga
    12181 tgaatttaac ccgccgtata tctttctacg ggattattta ggtatcgacc caacccaaac
    12241 tgtgtataca ttctcggaac atattatcaa cccggtgatg gtgacgcata tcatcttctc
    12301 actggttttt gcgatagggt attgtattgt cgcggaaatt ttcccgaaag tgaaattatg
    12361 gcaggggata ttagcagggc ttattgtgac agtggcagtc cacggtatat tttgcccggc
    12421 gctgaattta acgccgccgc tcactcaatt gccttttgac gaatatgcct ctgaaatcct
    12481 ggggcatatt ttctggttct ggataataga aattatgcgc cgcgacttac gtaatcgcat
    12541 tactcatgag ccagatccgg aagtcgcaat ccggtgaata accgcataaa aaagccccct
    12601 gcgaaggggg caaagcaaac tatggcaatg tttcgttggt tatacctggt gctagcgata
    12661 aatatccgcg ctggcgtgca tattgccgtt actccccggt tcccgcatca gaattacgtg
    12721 gtagtacgtt actccccggt tcccgcatca gaattacgtg gtagtacgtt gcgccctgcg
    12781 catctgtctc ttcatttaat gcctgacgcg cttcgctttc agtggcgaaa ttatgattga
    12841 tataaatcac gcctaagctt tgcacatcat ccatgtttct ggcctggtgg ttattaattt
    12901 caatggctgc ccatgtattt gcacttagca aaagcacagc cagaagggct aaaactcgac
    12961 tgaacataga tacctcctcg acggctgact ttgtgtgctc tcctctgtga tgatcttctg
    13021 atttaatttt aatcaatgat aaagaagttg atggtgacca tttctgatgc agttgttcaa
    13081 aaaaacacca tgatgaagtg tgatgaactc caaatcagcg gtttagaggt taattgcgaa
    13141 aggggagatt tatttcggct ctgcccttga gtttagcggg gcatacaagt actataacgc
    13201 gtcatttttc agccgacctt taacacgttc cttgcctccc cgggattcgg ctgacccaga
    13261 caggaggctg aataatccgt aaggagcaat tcgatgcgtc attacgaaat cgtttttatg
    13321 gtccatcctg accagagcga acaggttccg ggcatgatcg agcgctacac tgctgccatc
    13381 actggtgcag aaggcaagat ccaccgtctg gaagactggg gccgccgtca gctggcttac
    13441 ccgatcaaca aactgcacaa agcacactac gttctgatga acgttgaagc tccgcaggaa
    13501 gtgatcgatg agctggaaac taccttccgc ttcaacgatg ccgttatccg cagcatggtt
    13561 atgcgtacca agcacgctgt taccgaagca tctccgatgg ttaaagcgaa agacgagcgc
    13621 cgtgagcgtc gcgatgattt cgcaaacgaa accgctgatg atgctgaagc tggggattct
    13681 gaagagtaat ttctgatgac caaccgtctg gtgttgtccg gcaccgtgtg caggactccc
    13741 cttcgaaagg tcagtccatc aggaattcct cactgccagt tcgtgcttga gcatcgttct
    13801 gtgcaggagg aagccggttt tcaccggcag gcgtggtgcc aaatgcccgt tattgttagc
    13861 ggacacgaaa accaggccat tactcacagt ataacggtcg gcagtcgcat aaccgttcag
    13921 gggttcattt catgccacaa ggcaaagaac ggactgagca aaatggtttt gcatgccgag
    13981 cagattgaat tgatagattc tggagactag ccatatggca cgttatttcc gtcgtcgcaa
    14041 gttctgccgt ttcaccgcgg aaggcgttca agagatcgac tataaagata tcgctacgct
    14101 gaaaaactac atcaccgaaa gcggtaagat tgtcccaagc cgtatcaccg gtacccgtgc
    14161 aaaataccag cgtcagctgg ctcgcgctat caaacgcgct cgctacctgt ccctgctgcc
    14221 gtacactgat cgccatcagt aatcggtcac ggtccattaa tacgactttg agaggataag
    14281 gtaatgcaag ttattctgct tgataaagta gcaaacctgg gtagcctggg tgatcaggta
    14341 aacgttaaag cgggctatgc tcgtaacttc ctggtaccgc agggtaaagc tgttccagct
    14401 accaagaaaa acattgaatt cttcgaagca cgtcgcgctg aactggaagc taaactggct
    14461 gaagttctgg cagctgctaa tgctcgcgct gagaaaatca atgcactgga aactgttacc
    14521 atcgcgtcta aagctggcga cgaaggtaaa ctgttcggtt ccatcggtac tcgcgacatc
    14581 gctgacgctg taactgcagc tggcgttgaa gtggctaaga gcgaagttcg tctgccgaac
    14641 ggcgttctgc gtaccactgg cgagcacgaa gtgagcttcc aggttcacag cgaagtattc
    14701 gcgaaagtga tcgtaaacgt agtagctgaa taattcgtta ttcaacgaga cgtaaaaacg
    14761 ccgaccagtg gtcggcgttt tgcttttgta gcgagcactt gcagatatcg ataagggcca
    14821 caataagcag ccctacgaag ataattactt agctgcctga atatcagcca gcattggggc
    14881 aagggctggg ttgtccttag cgaagtttgc gtacattggc tgtactttct cgataaacgg
    14941 agctttatct acctggtaga ctttcacgcc cattttatcc agttttgcaa attcttgttt
    15001 ctcttcctcg ctccacaagt ctttcatgta gctcattgat tctttagctg cttttaatat
    15061 aactgcctga ttttccgggc taagtttatc aaacgcagca ttgctcatca gaaccacatc
    15121 gggaaccata gtgtgttcgt caaggctgaa ggattttgcc acttcaccgt gacgcatggt
    15181 ggtcagcgcc atcacgctgt tttctgccat atcgaccaca ccctgctgta aggctgtata
    15241 caactcgccg taatccagtg gcgtcgggtt accgcccatg actttgatca tttcaactgc
    15301 gccagggctt ggctggacac ggactttcat gcctttcaga tcgtctggtt tcagtactgg
    15361 tttattgcca tagaaactgc gggcgcctcc atcataaaaa gtcagcccga aataaccttt
    15421 gcttttcgtt gagtcgagga tttttctccc gatatcgccc tgcatgacct gataataatg
    15481 atcacgatcg cggaacaaat aaggcaggct aaataattta taggaatttt caaatgcttc
    15541 catttctgcc gcattggttt ttaccagtgg aatagcgcca gaacgaatca gctccattgt
    15601 ttctcgttga gtacccaatg ttccatttgg ataaatacga atagtaatat caccgttaga
    15661 gtactctttg ttctttttcg caaagaactc catcgcttta tgaacaggat gagacttatc
    15721 ctgattatga ctcagtttta atgtcactgc ccaggactgg cttgcggata acataagaac
    15781 agcaccgata atgccagcgg atacttttat tttcatagat gttccttttc taataaatat
    15841 ggcggcaggt gccgccagtg aatgcgcatc agtagcccag tgcttttggc aatgccatac
    15901 tgatttgcgg aatataggtc accattgcca gcaccaatac catcacggca tagaatggca
    15961 gcataggttt gataatttta ttaatcggta atttagatac actacaaccg acaaataaca
    16021 cactgcctac aggcggagta caaataccga tacacatgtt cagtaccatg acaataccaa
    16081 agtgaatcgg gtccatgccc agttcggtga caacaggtaa aaatatcggc gtaaaaatca
    16141 gtaccgccgg tgtcatatcc ataaaagtgc cgataatcaa caggataata ttgataataa
    16201 gcaggattat tattgggttg tcagaaatag tgttaagcaa atcgagaatc aggaagggaa
    16261 cgtcagcgtt tgacattgcc ca
//
LOCUS       DAJVQG010000092        16062 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJVQG010000092 DAJVQG010000000
VERSION     DAJVQG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16062
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.092"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(1..553)
                     /locus_tag="OQM83_002635"
                     /pseudo
     CDS             complement(1..553)
                     /locus_tag="OQM83_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(787..2207)
                     /gene="espX1"
                     /locus_tag="OQM83_002636"
                     /pseudo
     CDS             complement(787..2207)
                     /gene="espX1"
                     /locus_tag="OQM83_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
     gene            complement(2692..2955)
                     /gene="rpsT"
                     /locus_tag="OQM83_002637"
     CDS             complement(2692..2955)
                     /gene="rpsT"
                     /locus_tag="OQM83_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCS1520866.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            3058..3276
                     /locus_tag="OQM83_002638"
     CDS             3058..3276
                     /locus_tag="OQM83_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCS1520867.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            3284..4225
                     /gene="ribF"
                     /locus_tag="OQM83_002639"
     CDS             3284..4225
                     /gene="ribF"
                     /locus_tag="OQM83_002639"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCS1520868.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKASMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            4268..7084
                     /gene="ileS"
                     /locus_tag="OQM83_002640"
     CDS             4268..7084
                     /gene="ileS"
                     /locus_tag="OQM83_002640"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCS1520869.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            7084..7578
                     /gene="lspA"
                     /locus_tag="OQM83_002641"
     CDS             7084..7578
                     /gene="lspA"
                     /locus_tag="OQM83_002641"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCS1520870.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            7703..8152
                     /gene="fkpB"
                     /locus_tag="OQM83_002642"
     CDS             7703..8152
                     /gene="fkpB"
                     /locus_tag="OQM83_002642"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1520871.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            8154..9104
                     /gene="ispH"
                     /locus_tag="OQM83_002643"
                     /gene_synonym="lytB"
     CDS             8154..9104
                     /gene="ispH"
                     /locus_tag="OQM83_002643"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCS1520872.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            9170..10084
                     /gene="rihC"
                     /locus_tag="OQM83_002644"
     CDS             9170..10084
                     /gene="rihC"
                     /locus_tag="OQM83_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCS1520873.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            10251..11072
                     /gene="dapB"
                     /locus_tag="OQM83_002645"
     CDS             10251..11072
                     /gene="dapB"
                     /locus_tag="OQM83_002645"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCS1520874.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
     gene            11528..12676
                     /gene="carA"
                     /locus_tag="OQM83_002646"
     CDS             11528..12676
                     /gene="carA"
                     /locus_tag="OQM83_002646"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCS1520875.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            12694..15915
                     /gene="carB"
                     /locus_tag="OQM83_002647"
     CDS             12694..15915
                     /gene="carB"
                     /locus_tag="OQM83_002647"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCS1520876.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
BASE COUNT         3920 a         3776 c         4253 g         4113 t
ORIGIN      
        1 agaaaggaat ggagataaac agattaaaac gctcctcttc tttattattt tcatcataag
       61 attggctggc agagaaagta taactaatgt gttgccagtt attggagtaa ctgaattggt
      121 aatctttagc atttccactt cgcccccagt aattccgcca aagcgcactt aatgatacat
      181 ttcccagatt attggataaa ggttgcataa tattggcaga aaggctattt tttctgccaa
      241 tatcataaaa atcatcataa tcactctgat gatagtgttt atcattttcg taaagatggt
      301 cgctgaatgt cctgtaatcc tgcgaagcat aacgccaggc ggccacgcta aatcgagtgg
      361 ctgtttgtac tagatattta ttataggcga cctgatagct cgtaccttcg tgtctggtat
      421 cattatttaa cttactactc gatcgggcag cgtaaaaaga gatggcaccc agcggtgtat
      481 tccaaccatt tcccagagtt atagcattat agttatcgga taaaatcgta cccgattatg
      541 tgatcaggtt gttattattt agattaatgt ttaaattttt taatttcttg atgtgaagtt
      601 gatgaaagtt tgaaaattta atattcttaa ttgttccctt ctttataaga agagcaaggt
      661 gccatcatta gagtgacaga gtaggatgtt tcagagcatg cttcagattg ttttttattt
      721 ctctgcagtg tattaataat catcacagat aatttcattc taaaattacc tctttctttg
      781 agtgttttaa tcctgatgaa ctatattttt tataaggagc atgcactcgt tgatgctctg
      841 aaggtatttt ctcatttttg atgatgtttt gtcgtaatcg tgcataaact tattaagttc
      901 tttatacaca tcattattta gctttacatt atcgcgaact aaagtttgta ccaatggtat
      961 caaaatttcg aaacgggacg gtttgtcatc aatacttgcc agggttttga atactcctgt
     1021 gtatctgtaa cctaagtgct tattgacaag ttgagtggtc catcggccag gcataatatc
     1081 ttcacagcgc aaatttttta tcatggtatt agtgaacgat gttccaaata tgttgctttt
     1141 tttaatgaag aggttgttta tttctccatt tgaaaagatg gtgtttgata agtcgctttc
     1201 aaaacatgat atattggtta atacacaaca aaaataatac ccatatgaaa ggttgctctt
     1261 ttctattgtt atatttgata gcaacatttt atgtatatta atattaagag cagtcaaata
     1321 ctggatcttt tgatcgttaa gttcatacat ctccagtgat ttattatagg gtaataaaag
     1381 tataaattcc tttatattat ctccgcagta agaaaagacg acattaaatt tattagtggc
     1441 catgttttca gttgagcagt aagctgattt ttctattaac cgactgggca cgcttttaaa
     1501 taaagataat tttccacata gttttgaata tgccgaattg ctagttttaa agttctgcca
     1561 cattattccc tggttatcat ctatcgatga ggaggggatt ttattgcatt gctcattgta
     1621 tagctcaggg aaaatatttt ttttaataag ctttttgatt tcctggttaa gatgactttt
     1681 tttcttgtct attgcactgt attttgcgtt ttgtgacgtt tcctgatgat cataaaatga
     1741 ctggagattt ctgcaggcct cataaaatat ttcaggagta tatagctgtg ggtatatttc
     1801 ttggttgaag tttgccttta aaaaattaac gaatttttca tggcattctt ggtatatctt
     1861 aatctcttca gaaagacatt cgcttgttct gtcatcaaaa acatccaatc taagtaatgt
     1921 gatacctttg tgtttaaaaa ataaaattga actgtatgtt ctatgagttt acatctataa
     1981 aaatatgcat ctctcagatg agtatattga aaaaaatgat gaaggactgg agatggttta
     2041 ggcaagttat ggcttctaaa aaagtcatca aacaaaatta agaactctgt tttatactcg
     2101 caaaatactt ttactaactc attttgtctt gttttattta ttattacatc atctactata
     2161 tttgatttat aaagacaatg tggcgaagtt gagataccat ttgtcattat cagtccttga
     2221 agttgatgaa tttttattaa tttaatttat gagtgaatgt gactactatc aaaaatagct
     2281 cattaatttt atgaagtaga atataaatag gtatttaacc tgtgttgatt gttgttaatt
     2341 atttacaaga tctaataagg agatatttat gaatggttaa agtgtatagg aatttaattc
     2401 aaaaatagag tggtcttaag tttaatacct caatgtgtat cacaatatcc acattctttg
     2461 taggggagtc tggagattga gtagatattc ttgttcagaa tgtatcagcc gatggttcta
     2521 cgattcttaa gccacgaaga gttcagaaag tgtaacggca tgtctgcaaa agccagcatc
     2581 ttttgactat ctggcaaccg gtagtgtgtt ctctcacgca tcataaaagc agcaggcata
     2641 aaaaaaaccc gcttgcgcgg gctttttcac aaagcttcag caaattggcg attaagccag
     2701 tttgttgatc tgtgcagtca ggttagcctt atgacgtgca gctttgtttt tgtggatcag
     2761 acctttagca gcctgacggt ccacgatcgg ttgcatttcg ttaaatgctt tctgtgcagc
     2821 agctttgtcg ccagcttcga tagctgcgta tactttcttg atgaaagtac gcatcataga
     2881 gcgacggctt gcgttgtgct tacgagcctt ttcagactga atggcgcgct tcttagctga
     2941 tttgatatta gccaaggtcc aactcccaaa tgtgttctat atggacaatt caaaggccga
     3001 ggaatatgcc cttttagcct tcttttgtca atggatttgt gcaaataagc gccgttaatg
     3061 tgccggcact cgttacgtag tgatggcgca ggattctacc agcttgcggg gtgtgaatac
     3121 agcttttccg cgataaaaat tgcagcaggc ggtcactttc ttcccgtgat ttgcgccatg
     3181 gcaatgaaaa gccacttctt tctgatttcg gtactcaatc gccggttaac cttgaccgct
     3241 gtacaaggta tactcggacg attttcactg ttttgagcca gacatgaagc tgatacgcgg
     3301 catacataat ctcagccagg ccccgcaaga agggtgtgtg ctgactattg gtaatttcga
     3361 cggcgtgcat cgcggtcatc gcgcgctgtt acagggcttg caggaagaag ggcgcaagcg
     3421 caacttaccg gtgatggtga tgctttttga acctcaacca ctggaactgt ttgctaccga
     3481 taaagccccg gcaagactga cccggctgcg ggaaaaactg cgttaccttg cagagtgtgg
     3541 cgttgattac gtgctgtgcg tgcgtttcga caggcgtttc gcggcgttaa ccgcgcaaaa
     3601 tttcatcagc gatcttctgg tgaagcattt gcgcgtaaaa tttcttgccg taggggatga
     3661 tttccgcttt ggcgctggtc gtgaaggcga tttcttgtta ttacagaaag ctagcatgga
     3721 atacggcttc gatatcacta gtacgcaaac tttttgcgaa ggtggcgtgc gtattagcag
     3781 caccgctgtg cgtcaggcgc tggcggatga caatctggct ctggcagaaa gtttactggg
     3841 gcacccgttt gccatctccg ggcgtgtagt ccacggtgat gaattagggc gcactatagg
     3901 tttcccgacg gcgaatgtac cgctacgccg tcaggtttcc ccggtgaaag gggtttatgc
     3961 ggtagaagtg ctgggcctcg gtgaaaagcc gttacccggc gtggcaaaca tcggaacacg
     4021 cccaacggtt gccggtattc gccagcagct ggaagtgcat ttgttagatg ttgcaatgga
     4081 cctttacggt cgccatatac aagtagtgct gcgtaaaaaa atacgcaatg agcagcgatt
     4141 tgcgtcgctg gacgaactga aagcgcagat tgcgcgtgat gaattaaccg cccgcgaatt
     4201 ttttgggcta acaaaaccgg cttaagcctg ttatgtaatc aaaccgaaac acggaaccga
     4261 gaatctgatg agtgactata aatcaaccct gaatttgccg gaaacagggt tcccgatgcg
     4321 tggcgatctc gccaagcgcg aaccgggaat gctggcgcgt tggactgatg atgatctgta
     4381 cggcatcatc cgtgcggcta aaaaaggcaa aaaaaccttc attctgcatg atggccctcc
     4441 ttatgcgaat ggcagcattc atattggtca ctcggttaac aagattctga aagacattat
     4501 cgtgaagtcc aaagggcttt ccggttatga ctcgccgtat gtgcctggct gggactgcca
     4561 cggtctgccg atcgagctga aagtagagca agaatacggt aagccgggtg agaaattcac
     4621 cgccgccgag ttccgcgcca agtgccgcga atacgcggcg acccaggttg acggtcaacg
     4681 caaagacttt atccgtctgg gcgtgctggg cgactggtcg cacccgtacc tgaccatgga
     4741 cttcaaaact gaagccaaca tcatccgcgc gctgggcaaa atcatcggca acggtcacct
     4801 gcacaaaggc gcgaagccag ttcactggtg cgttgactgc cgttctgcgc tggcggaagc
     4861 ggaagttgag tattacgaca aaacttctcc gtccatcgac gttgcttttc aggcggtcga
     4921 tcaggatgca ctgaaagcaa aatttgccgt aagcaacgtt aacggcccaa tctcgctggt
     4981 gatctggacc actacgccgt ggactctgcc tgcgaaccgc gcaatctcta ttgcacctga
     5041 tttcgactat gcgctggtgc agatcgacgg tcaggccgtg attctggcga aagatctggt
     5101 tgaaagcgta atgcagcgta tcggcgtgac cgattacacc attctcggca cggtaaaagg
     5161 tgcggagctt gagttgctgc gctttaccca tccgtttatg ggcttcgacg ttccggcaat
     5221 cctcggcgat cacgttaccc tagatgccgg taccggtgcc gttcacaccg cgcctggcca
     5281 cggcccggac gactatgtga tcggtcagaa atacggcctg gaaaccgcta acccggttgg
     5341 cccggacggc acttatctgc cgggcactta tccgacgctg gatggcgtga acgtcttcaa
     5401 agcgaacgac atcgtcgttg cgctgctgca ggaaaaaggc gcgctgctgc acgttgagaa
     5461 aatgcagcac agctatccgt gctgctggcg tcacaaaacg ccgatcatct tccgcgcgac
     5521 gccgcagtgg ttcgtcagca tggatcagaa aggtctgcgt gcgcagtcac tgaaagagat
     5581 caaaggcgtg cagtggatcc cggactgggg ccaggcgcgt atcgagtcga tggttgctaa
     5641 ccgtcctgac tggtgtatct cccgtcagcg cacctggggc gtaccgatgt cactgttcgt
     5701 gcacaaagac acggaagagc tgcatccgcg tactctcgaa ctaatggaag aagtggcaaa
     5761 acgcgttgaa gttgacggca tccaggcgtg gtgggatctt gatgcgaaag agatcctcgg
     5821 cgatgaagct gatcagtacg tgaaagtgcc ggacacattg gatgtatggt ttgactccgg
     5881 atctacccac tcttctgttg ttgacgtgcg tccggaattt gccggtcacg cagcggacat
     5941 gtatctggaa ggttctgacc agcaccgtgg ttggttcatg tcttccctaa tgatctccac
     6001 cgcgatgaaa ggcaaagcgc cgtatcgtca ggtactgacc cacggcttta ccgtggatgg
     6061 tcagggtcgc aagatgtcta aatccatcgg caataccgtt tcgccgcagg atgtgatgaa
     6121 caaactgggc gcggatattc tgcgtctgtg ggtggcatca accgactaca ccggtgaaat
     6181 ggccgtttct gacgagatcc tgaaacgtgc tgccgatagc tatcgtcgta tccgtaacac
     6241 cgcgcgcttc ctgctggcaa acctgaacgg ttttgatcca gcaaaagata tggtgaaacc
     6301 ggaagagatg gtggtactgg atcgctgggc cgtaggttgt gcgaaagcgg cacaggaaga
     6361 catcctcaag gcgtacgaag catacgattt ccacgaagtg gtacagcgtc tgatgcgctt
     6421 ctgctccgtt gagatgggtt ccttctacct cgacatcatc aaagaccgtc agtacaccgc
     6481 caaagcggac agtgtggcgc gtcgtagctg ccagactgcg ctgtatcaca tcgcagaagc
     6541 gctggtgcgc tggatggcac caatcctctc cttcaccgct gatgaagtgt ggggctacct
     6601 gccgggcgaa cgtgaaaaat acgtcttcac cggtgagtgg tacgaaggcc tgtttggcct
     6661 ggcagacagt gaagcgatga acgatgcgtt ctgggacgag ctgttgaaag tgcgtggcga
     6721 agtgaacaaa gtcattgagc aagcgcgtgc cgacaagaaa gtgggtggct cgctggaagc
     6781 ggcagtaacc ttgtatgcag aaccggaact gtcggcgaaa ctgaccgcgc tgggcgatga
     6841 attacgattt gtcctgttga cctccggcgc taccgttgca gactataacg acgcacctgc
     6901 tgatgctcag cagagcgaag tactcaaagg gctgaaagtc gcgttgagta aagccgaagg
     6961 tgagaagtgc ccacgctgct ggcactacac ccaggatgtc ggcaaggtgg cggaacacgc
     7021 agaaatctgc ggccgctgtg tcagcaacgt cgccggtgac ggtgaaaaac gtaagtttgc
     7081 ctgatgagtc aatcgatctg ttcaacaggg ctacgctggc tgtggctggt ggtagtcgtg
     7141 ctgattatcg atctgggcag caaatacctg atcctccaga actttgctct gggggatacg
     7201 gtcccgctgt tcccgtcgct taatctgcat tatgcgcgta actatggcgc ggcgtttagt
     7261 ttccttgccg atagcggcgg ctggcagcgt tggttctttg ccggtattgc gattggtatt
     7321 agcgtgatcc tggcagtgat gatgtatcgc tcgaaggcca cgcagaagct aaacaatatc
     7381 gcttacgcgc tgattattgg cggcgcgctg ggcaacctgt tcgaccgcct gtggcacggc
     7441 ttcgttgtcg atatgatcga cttctacgtc ggcgactggc acttcgccac cttcaacctt
     7501 gccgatactg ccatctgtgt cggtgcggca ctgattgtgc tggaaggttt tttgccttct
     7561 agagcgaaaa aacaataata aaccctgccg gatgcgatgc tgacgcatct tatccggcct
     7621 acagattgct gcgaaatcgt aggccggata aggcgtttac gccgcatccg gcaaaaatcc
     7681 tcaaatataa gagcaaacct gcatgtctga atctgtacag agcaatagcg ccgtcctggt
     7741 gcacttcacg ctaaaactcg acgatggcac caccgccgag tctacccgca acaacggtaa
     7801 accggcgctg ttccgcctgg gtgatgcttc tctttctgaa gggctggagc aacacctgtt
     7861 ggggctgaaa gtgggcgata aaaccacctt ctcgttggag ccagatgcgg cgtttggcgt
     7921 gccgtcaccg gacctgattc agtacttttc ccgccgtgaa tttatggatg caggcgagcc
     7981 agaaattggc gcaatcatgc tttttaccgc aatggatggc agtgagatgc ctggcgtgat
     8041 ccgcgaaatt aacggcgact ccattaccgt tgatttcaac catccgctgg ccgggcagac
     8101 cgttcatttt gatattgaag tgctggaaat cgatccggca ttggaggcgt aacatgcaga
     8161 tcctgttggc caacccgcgt ggtttttgtg ccggggtaga ccgcgctatc agcattgttg
     8221 aaaacgcgct ggccatttac ggcgcaccga tatatgtccg tcacgaagtg gtacataacc
     8281 gctatgtggt cgatagcttg cgtgagcgtg gggctatctt tattgagcag attagcgaag
     8341 taccggacgg cgcgatcctg attttctccg cacacggtgt ttctcaggcg gtacgtaacg
     8401 aagcaaaaag ccgcgatttg acggtgtttg atgccacctg tccgctggtg accaaagtgc
     8461 atatggaagt cgcccgcgcc agccgtcgtg gcgaagagtc gattctcatc ggccacgccg
     8521 ggcatccgga agtggaaggt acaatgggcc agtacagtaa cccggaaggg ggaatgtatc
     8581 tggtcgaatc gccggacgat gtgtggaaac tgacggtcaa aaacgaagag aagctctcct
     8641 ttatgaccca gaccacgctg tcggtggatg acacgtctga tgtgatcgac gcgctgcgta
     8701 aacgcttccc gaaaattgtc ggtccgcgca aagatgacat ctgctacgcc acgactaacc
     8761 gtcaggaagc ggtacgcgcc ctggcagaac aggcggaagt tgtgttggtg gtcggttcga
     8821 aaaactcctc caactccaac cgtctggcgg agctggccca gcgtatgggc aaacgcgcgt
     8881 ttttgattga cgatgcgaaa gatatccagg aagagtgggt gaaagaggtt aaatgcgtcg
     8941 gcgtgactgc gggcgcatcg gctccggata ttctggtgca aaatgtggtg gcacgtttgc
     9001 agcagctggg cggtggtgaa gccattccgc tggaaggccg tgaagaaaac attgttttcg
     9061 aagtgccgaa agagctgcgt gtcgatattc gtgaagtcga ttaagtcatt agcagcctaa
     9121 gttatgcgaa aatgccggtc ttgttaccgg cattttttat ggagaaaaca tgcgtttacc
     9181 tatcttcctc gatactgacc ccggcattga cgatgccgtc gccattgccg ccgcgatttt
     9241 tgcccccgaa ctcgacctgc aactgatgac caccgtcgcg ggtaatgtct cggttgagaa
     9301 aaccacccgc aacgccctgc aactgctgca tttctggaat gcggagattc cgctcgccca
     9361 gggagccgct gtgccgctgg tacgcgcacc gcgtgatgca gcatctgtgc acggcgaatc
     9421 gggaatggct ggctacgact ttgttgagca caaccgaaag ccaatcggga taccagcgtt
     9481 tctggcgatt cgtgatgccc tgatgcgtgc accagagcct gttaccctgg tggccattgg
     9541 cccgttaacc aatattgcgc tgttactttc acaatgcccg gaatgtaaac cgtatattcg
     9601 tcgtctggtg atcatgggcg gttcttctgg tcgcggcaac tgtacaccaa atgccgagtt
     9661 taatatcgct gccgatccag aagcggctgc ctgtgtcttc cgtagcggaa tcgaaatcgt
     9721 catgtgcggt ctggatgtca ccaatcaggc aatattaacc cctgactatc tcgctacttt
     9781 gccggagttg aatcgcaccg gaaaaatgct tcatgcactg tttagccact accgtagcgg
     9841 cagtatgcaa agcggtctgc gaatgcacga tctctgcgcc atcgcctggc tggtgcgccc
     9901 ggacttattc accctcaagc cgtgttttgt cgcggtagaa actcaaggcg aatttacctc
     9961 aggcacgacg gtggttgata tcgacggttg cctgggcaaa ccagccaatg taaaggtggc
    10021 attggatctg gatgtgaaag gcttccagca gtgggtggct gaggtgctgg ctctggcgtc
    10081 gtaacctgtc acatgttatc ggcatgcagt cattcatcga ctcatgcctt tcactgatat
    10141 ccctccctgt ttatcattaa tttctaatta tcagcgtttt tggctggcgg cgtagcgatg
    10201 cgctggttac tctgaaaacg gtctatgcaa attaacaaaa gagaatagct atgcatgatg
    10261 caaacatccg cgttgccatc gcgggagccg gggggcgtat gggccgccag ttgattcagg
    10321 cggcgctggc attagaaggc gtgcagctgg gcgcggcgct ggagcgcgaa ggatcttctt
    10381 tactgggcag cgacgccggt gagctggctg gtgcagggaa aacaggcgtt accgtgcaaa
    10441 gcagcctcga tgcggtaaaa gatgattttg atgtgtttat cgattttacc cgtccggaag
    10501 gtacgctgaa ccatctcgct ttttgtcgcc agcatggcaa agggatggtg atcggcacta
    10561 cggggtttga cgaagccggt aaacaggcga ttcgtgacgc cgccgccgat attgcgattg
    10621 tctttgccgc caattttagc gttggcgtta acgtcatgct taagctgctg gagaaagcag
    10681 ccaaagtgat gggtgactac accgatatcg aaattattga agcacatcat agacataaag
    10741 ttgatgcgcc gtcaggcacc gcactggcaa tgggagaggc gatcgcccac gcccttgata
    10801 aagatctgaa agattgcgcg gtctacagtc gtgaaggcca caccggtgaa cgtgtgcctg
    10861 gcaccattgg ttttgccacc gtgcgtgcag gtgacatcgt tggtgaacat accgcgatgt
    10921 ttgccgatat tggcgagcgt ctggagatca cccataaggc gtccagccgt atgacatttg
    10981 ctaacggcgc ggtaagatcg gctttgtggt tgagtggtaa ggaaaatggt ctttttgata
    11041 tgcgagatgt acttaatctc aataatttgt aaccacaaaa tatttgttat ggtgcaaaaa
    11101 taacacattt aatttattga ttataaaggg ctttaatttt tggccctttt atttttagtg
    11161 ttatgttttt aaatattcta taagtgccaa aaattacatg ttttgtcttc tatttttgtt
    11221 gttttaatgt aaattttgac catttggtcc actttttcct gctcgttttt atttcatgca
    11281 atcttcttgc tgcgcaagcg ttttccagaa caggttatat gatctttttg tcgcttaatg
    11341 cctgtaaaat atgcatgagc cacaaaataa tataaaaaat accgccatta agttgacttt
    11401 tagcgcccat atctccagaa tgccgccgtt tgccagaaat tcgtcggtaa gcagatttgc
    11461 attgatttac gtcatcattg tgaattaata tgcaaataaa gtgagtgaat attctctgga
    11521 gggtgttttg attaagtcag cgctattggt tctggaagac ggaacccagt ttcacggtcg
    11581 ggccataggg gcaacaggtt cggcggttgg ggaagtcgtt ttcaatactt caatgaccgg
    11641 ttatcaagaa atcctcactg atccttccta ttctcgtcaa atcgttactc ttacttatcc
    11701 ccatattggc aatgtcggca ctaatgacgc cgatgaagaa tcttctcagg tacatgcaca
    11761 aggtctggtg attcgcgatc tgccgctgat tgccagcaac ttccgtaata ccgaagacct
    11821 ctcttcttac ctgaagcgcc ataacatcgt ggcgattgcc gatatcgata cccgtaagct
    11881 gacgcgttta ctgcgcgaga aaggcgcaca gaatggctgc attatcgcag gcgataaccc
    11941 ggatgcggcg ttggcgttag aaaaagcccg cgcgttccca ggtctgaatg gcatggatct
    12001 ggcaaaagaa gtgaccaccg cagaagccta tagctggaca caagggagct ggacgttgac
    12061 cggcggcctg ccagaagcga aaaaagaaga cgagctgccg ttccacgtcg tggcttatga
    12121 ttttggtgcc aagcgcaaca tcctgcggat gctggtggat agaggctgtc gcctgaccat
    12181 cgttccggcg caaacttctg cggaagatgt gctgaaaatg aatccagacg gcatcttcct
    12241 ctccaacggt cctggcgacc cggccccgtg cgattacgcc attaccgcca tccagaaatt
    12301 cctcgaaacc gatattccgg tattcggcat ctgcctcggt catcagctgc tggcgctggc
    12361 gagcggtgcg aagactgtca aaatgaaatt tggtcaccac ggcggcaacc atccggttaa
    12421 agatgtggag aaaaacgtgg tgatgatcac cgcccagaac cacggttttg cggtggatga
    12481 agcaacatta cctgcaaacc tgcgtgtcac gcataaatcc ctgttcgacg gtacgttaca
    12541 gggcattcat cgcactgata aaccggcgtt cagcttccag ggtcacccgg aagccagccc
    12601 tgggccacac gacgcagcgc cgttgttcga ccactttatc gagttaattg agcagtaccg
    12661 taaaaccgct aagtaatcag gagtaaaaga gccatgccaa aacgtacaga tataaaaagt
    12721 atcctgattc tgggtgcggg cccgattgtt atcggtcagg cgtgtgagtt tgactactct
    12781 ggcgcgcaag catgtaaagc cctgcgcgaa gagggttacc gcgtcattct ggtgaactcc
    12841 aacccggcga ccatcatgac cgacccggaa atggccgatg cgacctacat cgagccgatt
    12901 cactgggaag tagtacgcaa gattattgaa aaagagcgcc cggacgcggt gctgccaacg
    12961 atgggcggtc agacggcgct gaactgcgcg ctggagctgg agcgtcaggg cgtgttggaa
    13021 gagttcggcg tgactatgat tggtgcgacc gccgatgcga ttgataaagc agaagaccgc
    13081 cgtcgtttcg acgtagcgat gaagaaaatt ggtctggaaa ccgcgcgttc cggtatcgca
    13141 cacacgatgg aagaagcgct ggcggttgcc gctgacgtgg gcttcccgtg cattattcgc
    13201 ccatccttta ccatgggcgg tagcggcggc ggtatcgctt ataaccgcga agagtttgaa
    13261 gaaatttgcg cccgcggtct ggatctctcc ccaaccaaag agctgctgat tgatgagtcg
    13321 ctgatcggct ggaaagagta cgagatggaa gtggtgcgtg ataaaaacga caactgcatc
    13381 atcgtctgct ctatcgaaaa ctttgatgcg atgggcatcc ataccggtga ctccatcact
    13441 gtcgcgccag cccaaacgct gaccgacaaa gaatatcaaa tcatgcgtaa cgcctcgatg
    13501 gcggtgctgc gtgaaatcgg cgttgaaacc ggtggttcca atgtccagtt tgcggtgaac
    13561 ccgaaaaacg gtcgcctgat tgttatcgaa atgaacccac gcgtgtcccg ttcttcggcg
    13621 ctggcgtcga aagcgaccgg tttcccgatt gctaaagtgg cggcgaaact ggcggtgggt
    13681 tacaccctcg acgaactgat gaacgacatc actggcggac gtactccggc ctccttcgag
    13741 ccgtccatcg actatgtggt tactaaaatt cctcgcttca acttcgaaaa attcgctggt
    13801 gctaacgacc gtctgaccac tcagatgaaa tcggttggcg aagtgatggc gattggtcgc
    13861 acgcagcagg aatccctgca aaaagcgctg cgcggcctgg aagtcggtgc gactggattc
    13921 gacccgaaag tgagcctcga tgacccggaa gcgttaacca aaatccgtcg cgaactgaaa
    13981 gatgctggcg cagagcgtat ctggtacatc gccgatgcct tccgtgcggg cctgtctgtg
    14041 gacggcgtgt tcaacctgac caatattgac cgctggttcc tggtacagat tgaagaactg
    14101 gtgcgtctgg aagagaaagt agcggaagtg ggcatcactg gcctgaacgc tgaattcctg
    14161 cgccagctga aacgcaaagg ctttgccgat gcgcgtctgg caaaactggc gggcgtgcgc
    14221 gaagcggaaa tccgcaagct gcgcgaccag tatgacctgc acccggttta taagcgcgtg
    14281 gatacctgtg cggcagagtt cgccaccgac accgcttaca tgtactccac ttatgaagaa
    14341 gagtgcgaag cgaatccgtc taccgaccgt gaaaaaatca tggttctcgg cggcggcccg
    14401 aaccgtatcg gtcagggtat cgaattcgac tactgctgtg tacacgcctc gctggcgctg
    14461 cgcgaagacg gttacgaaac cattatggtt aactgtaacc cggaaaccgt ctccaccgac
    14521 tacgacactt ccgatcgcct ctacttcgag ccggtaactc tggaagatgt gctggaaatc
    14581 gtgcgtattg agaagccgaa aggcgttatc gttcagtacg gcggtcagac cccgctgaaa
    14641 ctggcgcgcg cgctggaagc tgctggcgta ccggttatcg gcaccagccc ggatgctatc
    14701 gaccgtgcag aagaccgtga acgcttccag catgcggttg agcgtctgaa actgaaacaa
    14761 ccggcgaacg ccaccgttac cgctattgaa atggcggtag agaaggcgaa agagattggc
    14821 tacccactgg tggtacgtcc gtcttacgtt ctcggcggtc gggcgatgga aatcgtctat
    14881 gacgaagctg acctgcgtcg ctacttccag acggcggtca gcgtgtctaa cgatgcgcca
    14941 gtgttgctgg atcacttcct tgatgacgcg gtagaagttg acgtggatgc catctgcgac
    15001 ggcgaaatgg tgctgattgg cggcatcatg gagcacatcg agcaggcggg cgtgcactcc
    15061 ggtgactccg catgttctct gccagcctac accttaagtc aggaaattca ggatgtgatg
    15121 cgccagcagg tgcagaaact ggccttcgaa ttgcaggtgc gcggtctgat gaacgtgcag
    15181 tttgcggtga aaaacaacga agtctacctg attgaagtta acccgcgtgc ggcgcgtacc
    15241 gttccgttcg tctccaaagc caccggcgta ccgctggcaa aagtggcggc gcgtgtgatg
    15301 gctggcaaat cgctggctga gcagggcgta accaaagaag ttatcccgcc gtactactcg
    15361 gtgaaagaag tggtgctgcc gttcaataaa ttcccgggtg ttgacccgct gttagggcca
    15421 gaaatgcgct ccaccgggga agtgatgggc gtgggccgca ccttcgctga agcgtttgcc
    15481 aaagcgcagc tgggcagcaa ctccaccatg aagaaacacg gtcgtgcgtt gctttccgtg
    15541 cgcgaaggcg ataaagaacg cgtggtggac ctggcggcaa aactgctgaa acagggcttc
    15601 gagctggatg cgacccacgg cacggcgatt gtgctgggcg aagcgggcat taatccgcgt
    15661 ctggtgaaca aggtgcatga aggccgtccg cacattcagg accgcatcaa gaatggcgaa
    15721 tatacctaca tcatcaacac cacttcaggc cgtcgcgcga ttgaagactc ccgcgtgatc
    15781 cgtcgcagtg cgctgcaata taaagtgcat tatgacacca ccctgaacgg tggtttcgct
    15841 accgcgatgg cgctgaatgc cgacgcgacc gaaaaagtaa tttcggtgca ggaaatgcac
    15901 gcgcagatca aataatagcg tgtcatggcg gatatttttc atccgctaat ttgatcgaat
    15961 aagtaatacg gttctctgat gaggaccgtt ttttttgccc attaattaaa tcttttgggg
    16021 aatcgatatt tttgatgaca taagcaggat ttagctcaca ct
//
LOCUS       DAJVQG010000093        15582 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJVQG010000093 DAJVQG010000000
VERSION     DAJVQG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15582
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.093"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..2065
                     /locus_tag="OQM83_002648"
     CDS             <1..2065
                     /locus_tag="OQM83_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS1520877.1"
                     /translation="GYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNSG
                     LNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSVR
                     FRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITDL
                     QLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHSD
                     FVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGDV
                     FDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYDI
                     ADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLRR
                     ISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFASN
                     NTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQAG
                     ASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMTP
                     YRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQPL
                     TFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDESR
                     NYICQ"
     gene            2081..3115
                     /locus_tag="OQM83_002649"
     CDS             2081..3115
                     /locus_tag="OQM83_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HCS1520878.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFML
                     ESNGSPVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(3197..3535)
                     /gene="rcnB"
                     /locus_tag="OQM83_002650"
     CDS             complement(3197..3535)
                     /gene="rcnB"
                     /locus_tag="OQM83_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCS1520879.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3754..4578)
                     /gene="rcnA"
                     /locus_tag="OQM83_002651"
     CDS             complement(3754..4578)
                     /gene="rcnA"
                     /locus_tag="OQM83_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCS1520880.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4699..4971
                     /gene="rcnR"
                     /locus_tag="OQM83_002652"
                     /pseudo
     CDS             4699..4971
                     /gene="rcnR"
                     /locus_tag="OQM83_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
     regulatory      5063..5159
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5194..5982
                     /gene="thiM"
                     /locus_tag="OQM83_002653"
     CDS             5194..5982
                     /gene="thiM"
                     /locus_tag="OQM83_002653"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCS1520881.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5979..6779
                     /gene="thiD"
                     /locus_tag="OQM83_002654"
     CDS             5979..6779
                     /gene="thiD"
                     /locus_tag="OQM83_002654"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCS1520882.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6844..7662
                     /locus_tag="OQM83_002655"
     CDS             6844..7662
                     /locus_tag="OQM83_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCS1520883.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7714..8460
                     /locus_tag="OQM83_002656"
     CDS             7714..8460
                     /locus_tag="OQM83_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1520884.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8434..9399)
                     /locus_tag="OQM83_002657"
     CDS             complement(8434..9399)
                     /locus_tag="OQM83_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS1520885.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9396..10400)
                     /locus_tag="OQM83_002658"
     CDS             complement(9396..10400)
                     /locus_tag="OQM83_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCS1520886.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10397..11674)
                     /locus_tag="OQM83_002659"
     CDS             complement(10397..11674)
                     /locus_tag="OQM83_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1520887.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11931..12983
                     /gene="fbaB"
                     /locus_tag="OQM83_002660"
     CDS             11931..12983
                     /gene="fbaB"
                     /locus_tag="OQM83_002660"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCS1520888.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13302..14156
                     /gene="gatY"
                     /locus_tag="OQM83_002661"
     CDS             13302..14156
                     /gene="gatY"
                     /locus_tag="OQM83_002661"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCS1520889.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14185..15442
                     /gene="gatZ"
                     /locus_tag="OQM83_002662"
                     /pseudo
     CDS             14185..15442
                     /gene="gatZ"
                     /locus_tag="OQM83_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15452..>15582
                     /locus_tag="OQM83_002663"
     CDS             15452..>15582
                     /locus_tag="OQM83_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCS1520890.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAR"
BASE COUNT         4088 a         3728 c         3987 g         3779 t
ORIGIN      
        1 tggctatgtt ccaccggaaa actgggagcg gggtattaat gcgttttata cctcttatta
       61 tgtgagtcag tattacagtg actataaagc gtcgggtaat agtaagagta catatgtacg
      121 ttttaacagc gggttaaatt tactggggtg gcaactgcat tctgatgcca gtttcagtaa
      181 aacaaataac aatccagggg tgtggaaaag caataccctg tatctggaac gtggatttgc
      241 ccaacttctc ggcacgcttc gcgtgggtga tatgtacaca tcaagcgata tttttgattc
      301 tgttcgcttc agaggtgtgc ggttgtttcg tgatatgcag atgttgccta actcgaaaca
      361 aaattttacg ccacgggtgc aggggattgc tcagagtaac gcgctggtaa ctattgaaca
      421 gaatggtttt gtggtttatc agaaagaggt tcctcctggc ccgtttgcga ttacagattt
      481 gcagttggcc ggtggtggag cagatcttga tgtcagcgtg aaagaggcgg acggttcggt
      541 aaccacctat ctggtgcctt atgcagcggt gccaaatatg ctgcaacccg gcgtgtcgaa
      601 atatgatttt gcggcgggtc gtagccatat tgaaggggcg agcaaacata gtgattttgt
      661 ccaggcgggt tatcagtatg gttttaataa cttattgacg ttgtatggtg gctcgatggt
      721 tgcgaataat tattacgcgt tcactttggg gactggctgg aatacacgca ttggtgccat
      781 ttccgtcgat gccacgaagt cgcatagtaa acaagacaac ggcgatgtgt ttgacgggca
      841 aagttatcaa attgcctaca acaaatttgt gagccaaacg tcgacgcgtt ttggtctggc
      901 ggcctggcgt tattcgtcgc gtgattaccg gacatttaac gatcacgttt gggcaaacaa
      961 taaagataat tatcgccgtg atgaaaacga tgtctatgac attgccgatt attaccagaa
     1021 tgattttggt cgcaaaaata gcttttccgc caatatgagc cagtcattgc cagaaggttg
     1081 ggggtctgtg tcattaagta cgttatggcg agattactgg gggcgtagcg gcagtagtaa
     1141 ggattatcag ttgagttatt ccaacaacct gcgacggata agctataccc tcgcggcaag
     1201 ccaggcttat gacgagaatc atcatgaaga gaaacgtttt aatattttta tatcgattcc
     1261 ctttgattgg ggtgatgacg tttcgacgcc tcgtcggcaa atatatatgt ctaactcaac
     1321 gacgtttgat gatcaggggt ttgcctcaaa taatacggga ttatcaggaa cagtagggag
     1381 tcgggatcag ttcaattatg gtgtcaacct gagtcatcaa catcagggaa atgaaacgac
     1441 agctggagcg aatttaacct ggaacgcgcc ggtggcaaca gtgaatggca gttatagtca
     1501 gtcgagtact tatcgacagg ctggagccag tgtttcaggg ggcattgtcg cctggtcggg
     1561 tggcgttaat ctggcgaacc gtctttccga aacgtttgct gtgatgaatg cgccaggaat
     1621 taaagatgct tatgtcaatg ggcaaaaata tcgcacaaca aaccgtaatg gagtggtggt
     1681 atacgacgga atgacacctt atcgggaaaa tcacctgatg ctggatgtgt cgcaaagcga
     1741 tagcgaagca gaattacgtg gcaaccggaa aattgccgca ccttatcgcg gcgcggttgt
     1801 actggttaat tttgataccg atcagcgcaa gccctggttt ataaaagcgt taagagcgga
     1861 tgggcaacca ttaacgtttg gttatgaagt caatgatatc cacggtcata atattggcgt
     1921 tgtcggccag ggaagccagt tatttattcg caccaatgaa ataccgccat cggttaatgt
     1981 agcaattgat aagcaacaag gactttcatg cacaatcacc ttcggtaaag agattgatga
     2041 aagtagaaat tatatttgcc agtaatagtc aggtggttct atggaaattc gcataatgct
     2101 atttatatta atgatgatgg ttatgcctgt gagctatgcg gcatgttata gtgagttatc
     2161 tgttcagcac aatttggttg ttcaggggga ttttgcactt actcaaacac aaatagcgac
     2221 atatgagcat aattttaatg attcgtcatg cgtgagtaca aatactatca cccctatgag
     2281 ttcgtcggat attattgttg ggctttataa cgataccata aaattaaatt tacattttga
     2341 atggaccaat aaaaacaaca tcacgttgtc aaataatcag accagtttca ccagtggtta
     2401 ttcagttacg gtgacacctg cggccagtaa tgcaaaagtg aatgtttctg cggggggcgg
     2461 cggttcagtg atgattaatg gtgttgcgac attatccagt gcttcatcat cgacacgcgg
     2521 gagtgccgca gtacaatttc tactgtgttt attaggtggc aagtcatggg atgcatgtgt
     2581 aaatagctac agaaatgcat tggcacaaaa tgcaggtgtc tattccttta atctgacatt
     2641 gtcatacaac ccgataacca caacctgcaa accggacgat ttattaatta ctttagacag
     2701 tattcccgtt tcacaattac cagccacagg taacaaagca acaataaata gtaaacaagg
     2761 ggatattatt ctgcgttgta aaaatttatt aggtcaacaa aatcaaacat cacggaaaat
     2821 gcaggtgtat ttatcaagtt ctgatttgtt agccggcagt aacacgatcc tgaaaggtgc
     2881 agaaaataat ggagtcggat ttatgcttga aagcaatggt tctccagtca catttttaaa
     2941 tatcactgat aacagcaaag gatacacaaa tttaaaagaa attgcggcaa agtcaaaact
     3001 tacagataca acggtttcaa ttccgataac tgccagttac tacgtctacg atacaaacaa
     3061 agttaaatct ggcgcactgg aggcaaccgc attaatcaac gtgaaatacg actaataaca
     3121 agcaagacga gcaagtggct aataataaaa aataacaagg tgtctgcatt tccccctcat
     3181 gatgaggggg ctttttttag cgatgataaa aaatctcacc gtcgtaggct ttaatgattt
     3241 taccgtcggt gtcgctgatc aacacgtacg cgccacccat ataggtccag tgcgtcccgg
     3301 catcaggcgc gggcaggtta cgtaactgcc actgcttaat gttgtactca tcggtgcggt
     3361 acatctctgg gacggtatca cctggtttga aatgcttaaa gtcagcaatg aaactactta
     3421 actcatactt agaaattccc gaggtattgg tcgaacccgc ggtggctggt gcggcccagg
     3481 cggcactggt aaccagcaaa agcacaccca gcaacatctt atttttaatg gtcataaact
     3541 ctccactttg gggatctgta atatcagtcg catatctttc ctgccataca atataactga
     3601 caagcgatat tttgttagtg atcccatgat ttcgtaaaaa gtgtaaatga aaggatgccc
     3661 taaaagcatc cttagcgagc agagagtcag cggaaatagc tgatttaaga atcatcctgg
     3721 gctgaagata acatgcaagc ctaaaggatt gagttatcgc attatgccca tgaagccatg
     3781 tacgcccata tacacaccga ctaagccaat caacagactg gaaaaatagg gggcgcgttt
     3841 agcgagagtg ttaaatccgc tccagcgttt tgcgacctgc tgaacgctga ttgctgcgcc
     3901 aacgcctacg gtgacaagcg ttaacgccag gccaatgctg aaactgacga ccagtgttgc
     3961 gcccagtgtc agggctttca actgaatgca tatcaacagc acggtaattg ctgccgggca
     4021 ggggataagg ccaccggtta agccaaatag caaaatttgc cagttggtga cctctctacc
     4081 atcaaagcgt cgtttaatgt cattggcatg ggctcgtgca tgggcatcct gatactcgcc
     4141 atgttcgtga tggtgatgat gtccatggtc gtggtgatct tcgtgatcgt gatgatgatg
     4201 ctcataatca tactcgtgca tattctccag ccagttgcgt tcgccgcgcc aggtacgcca
     4261 gaacatccag aacgcggtgc caataatgat cactgcggaa atcagctgga gccacggttc
     4321 tgctgattga gcagtaaagc gcttgctgat caccatcccg ccaaaggcaa ttaaccagac
     4381 cactgcggta tgcgaaatag ttgctgccag tccgagcatc accgcttgtt taatggtgcc
     4441 tttgatggcg atgataaacg ccgccatcat cgtttttgag tgccctggtt ccaggccatg
     4501 aagcgcacca agtaagatgg cgctggggat gaagaaccag gcgtttcctt gctgaagaag
     4561 agttgtaaat tcggtcatga taataattct tagtattaat tcggcaatct gattctactc
     4621 cccccagtac ctgatactac cccccagtag attaatagtg ctatgatatt ttcatattct
     4681 tgttaaccag gtgttgccat gtctcataca atccgtgata aacagaaact gaaagcgcgt
     4741 gccagtaaga ttcagggcta ggtcgtggcg ctcaagaaaa tgctcgacga gccgcatgaa
     4801 tgcgctgcag ttttacaaca gattactgct atccgtggcg cggtaaacgg tctgatgcgg
     4861 gaagtgatta aaggtcatct gacggaacac atcgttcacc agggggatga gctaaaacgt
     4921 gaagaagatc tggatgtcgt tctgaaggtg ctggattcat atatcaaata atttattaac
     4981 gcgattgtaa aactgccgtt tttcctcgtt tacaacgcgt gcgctggaca ttaccatcct
     5041 cctctgcgat ttatcatcgc aaccaaacga ctcggggtgc ccttctgcgt gaaggctgag
     5101 aaatacccgt atcacctgat ctggataatg ccagcgtagg gaagtcacgg accaccaggt
     5161 cattgcttct tcacgttatg gcaggagcaa actatgcaag tcgacctgct gggttcagcg
     5221 caatctgcgc acgcgttaca cctttttcac caacattccc ctcttgtgca ctgcatgacc
     5281 aatgatgtgg tgcaaacctt taccgctaat accttgctgg cgctcggtgc atcgccagcg
     5341 atggttatcg aaaccgaaga ggccagtcag tttgcggcta tcgccagtgc cttgttgatt
     5401 aacgttggca cactgacgca gccacgcgct caggcgatgc gtgctgccgt tgagcaagca
     5461 aaaagctctc aaacaccctg gacgcttgat ccagtagcgg ttggtgcgct cgattatcgc
     5521 cgccattttt gtcatgaact tttatctttt aaaccggcag cgatacgtgg taatgcttcg
     5581 gaaatcatgg cattagctgg cattgctaat ggtggacggg gagtggatac cactgacgcc
     5641 gcagctaacg cgatacccgc tgcacaaaca ctggcacggg aaactggcgc aatcgtcgtg
     5701 gtcactggcg aggtggatta tgttaccgat ggccatcgcg ccgttggcat tcacggtggc
     5761 gatccgttaa tgaccaaagt ggtaggaact ggctgtgcat tatcggcggt tgtcgctgcc
     5821 tgctgtgcgt taccaggcga tacgctggaa aatgtcgcat ctgcctgtca ctggatgaaa
     5881 caagccggag agcgcgcagt cgccagaagc gaggggctag gcagttttgt tccacatttc
     5941 cttgatgcgc tctggcaatt gacgcaggag gtgcaggcat gaaacgaatt aacgctctga
     6001 cgattgccgg tactgatccg agtggtggtg cggggattca ggccgatctt aaaaccttct
     6061 cggcacttgg cgcttatggt tgctcagtta ttactgcact ggtggcgcaa aatacccgtg
     6121 gcgtacagtc ggtgtatcgc attgagcctg attttgtcgc cgcccagctc gattcggtgt
     6181 tcagcgatgt gcgaatcgat accactaaaa tcggtatgtt ggcggaaacc gatattgttg
     6241 aagcggtggc agaacggttg caacgttatc agatccaaaa cgtggtactc gacaccgtta
     6301 tgctggcaaa aagcggcgac ccactgcttt caccttcggc agttgccacg ctacgcagtc
     6361 gattattgcc acaggtttca ttaataacgc caaacttgcc cgaagcagcc gccttgctcg
     6421 acgcgccaca cgcgcgcacc gaacaggaaa tgctggaaca agggcgatcg ctgttggcga
     6481 tgggctgtgg cgcagtgcta atgaaaggtg gtcatctgga tgatgagcaa agcccggact
     6541 ggctgtttac ccgcgagggt gaacaacggt ttaccgcacc gcgcattatg accaaaaaca
     6601 cccacggtac tggttgtaca ctctctgcgg cgttggctgc actacgcccg cgccatacaa
     6661 actgggctga caccgtacag gaggcaaaaa gctggctttc atcggcgtta gcccaggccg
     6721 acacgctgga agttggtcac ggtattggtc cggttcacca cttccacgcc tggtggtgag
     6781 caccgtacta tagctgcttt ggttattgtc agcctttggc actgacattc agggatgaaa
     6841 ccaatgcagc taagaattac cagtcgaaaa aaactcaccg ctctattatg cgctcttggg
     6901 ctaatttcca tcgttgcaat ttatccgcgt cagacggtga attttttcta ttcgacagca
     6961 gttcagatta cagactacat ccacttctac ggttatcgcc cggttaaatc tttcgctatt
     7021 cgtattcctg ccagttacac cattcacgga atagatgttt cgcgctggca ggagcggatc
     7081 gactggcagc gtgtggcaaa aatgcgcgat aacggtattc gcttacaatt tgcctttatt
     7141 aaggcgacgg aaggcgaaaa gctggtggac ccctattttt cgcgtaactg gcaactaagc
     7201 cgcgaaaatg gcttgctgcg cggggcatat cattatttct ccccgtcggt agcagcaccg
     7261 gttcaggtgc gattatttct gcaaacggta gatttctcac aaggcgattt ccctgcggta
     7321 ctggacgtag aagagcgagg aaaattatcg gcaaaagaat tacgcaagag ggtaagtcag
     7381 tggctaaaaa tggtcgaaaa aagtacgggg aaaaagccga ttatttactc aggagccgtt
     7441 ttttatcaca cgaatctggc gggctatttc aatgaatatc cgtggtgggt ggctcactac
     7501 tatcaacgtc gtccggacaa tgacggcatg gcctggcgct tctggcagca ttccgaccgt
     7561 ggacaggtag atggcatcaa tggtccggtg gattttaatg tgtttaatgg cacggtggag
     7621 gagttgcagg gattcgttga tgggattaaa gaaacgcctt aaataagaag cagtgatata
     7681 ctcccaccag actccctggc aggaaaaaga caaatggaac aagcgcatac ccagctgatt
     7741 gcccaactga acgaacgtat tttagcggcg gataacacgc cgctttatat taagtttgcc
     7801 gaaacggtaa aaaatgccgt gcgcagcggg gtgctggagc atggcaatat tttgcccggt
     7861 gagcgtgatt taagtcagtt aaccggcgtt tcgcgcatta cggtgcgcaa ggcgatgcag
     7921 gcgctggaag aagagggcgt ggtgacgcgt tcgcgcggtt acggcacgca gatcaacaac
     7981 atcttcgaat actcgttgaa agaagcgcga ggtttttctc agcaggtggt gttgcgcggg
     8041 aaaaagcccg atacgctatg ggttaacaag cgtgtcgtga aatgtcccga agaagtcgcg
     8101 cagcagctgg cggtcgaagc aggaagtgat gtctttttgc ttaagcgtat tcgctatgtc
     8161 gatgaagaag cggtatcgat tgaggaatcg tgggttccgg cgcatttaat tcatgatgtt
     8221 gatgccatcg ggatttcact ttatgactat ttccgcagcc agcacattta cccacaacgt
     8281 acgcgttccc gcgttagcgc ccggatgccg gatgccgagt ttcagtcaca tattcagtta
     8341 gatagcaaaa taccggtgct ggtgatcaag caagtggcgc ttgaccaaca gcaacggcct
     8401 attgagtaca gcattagcca ctgtcgcagc gatctatacg tttttgtgtg cgaggagtag
     8461 ttcctcgcgc gttggcgcac aatcaccgcc ccgatgcccg acaacccacg acgccactgc
     8521 attgcccagt aatacggcat ccgccagtgg cagaccagag gccagcccgg caagtacgcc
     8581 acctgcatga ctgtcgcccg ccccaatggt gtctacaact tgcgttggaa atgccggaat
     8641 gcagccagaa gcgtcgttgc tgaaatacca tgcgccttct ttatcgagac gaacgatcaa
     8701 cggcgcggca aatttctcct gccattgctc gccaagtgtt gttatctctg cggataaagc
     8761 aaaacgttcg gcggcaatct cagcctcttg acgattgagc gacactaaag gtcgacaggc
     8821 catgatccgc gccagtaatg catccgggat atcgccaata cgtgggccaa aatcgataaa
     8881 cggcgtcacg tcttgcagct cttccagcca ttccactaac aattcgccgc agggcgaggc
     8941 cagttgataa ccggaaaaat agagcaggct gccaggcgca acggttaatc gcgccagcca
     9001 ctggcgattc cactgatttt ccacaccgct gaatgacata aaagtgcgtt cgccatccgg
     9061 ctcaaccagc gccagacacc agccgttatc accttcggcg ttatcgatca ggctgattaa
     9121 gccctctttt gccatccggt tgcgaataat ctccgcccac acgccctgac cgagcggcaa
     9181 ggcattaccc gcttcgatgc cgaggcgctt taacgccacg gcaatattca gtgcgcagcc
     9241 gccaacgtta acgctctgct gtttcagttc gatatcgcac ccacgccagg ggagggcata
     9301 agcgtcggcg atcacgtcaa tgaccgccgc gccgacgacc atcaccggct gacgcgtggt
     9361 taattcaggc agcagcgtgt gtaatcgagc gccgctcata ccgcctcccg ttgttgacga
     9421 tatttcgcca gcgctgtggc atagcggttg aaatcaagct gatttaccgc atccagttcc
     9481 gcctttaacg caggatcgat agcgttaacg ccatgcaacg cgccgcaaat tgccgtcgcc
     9541 atagcaccga tggtgtctgt gtcgccgcca aggttagcgc acaggacggc gcagcgattc
     9601 gggtcggttt gtgctagttc aaccagcgca atggcgcacg gaacggactc aatagtgctg
     9661 gtacctgcgc caacgacctg gtaaagctgt tcgctggcgg attcggtgcc gtcggcattg
     9721 cgcacaattt tcagcgcaat ctccagccgc gccgccagtg aggcgctgaa cgtagtgatg
     9781 cgtttttgct gtgcatgtcg cgcaattgaa ggcagggaat caacaatcgc tgaccagctt
     9841 tctccgtcaa tggcacgaga aatcgcccat gcgatgacta ccgcgcctgc aaccgccaga
     9901 tcggatttat gggtcgggct ggacgccagc gctacatcat caataaagga atcaacatca
     9961 cgcgccggaa gcaaacaacc taatggcgag acgcgcatcg ctgcgccgtt ggtcacgccg
    10021 ttgttttcca gttcagcaac gggtttaccg tcgcgaatgg cgttaagcgc aatcttcgag
    10081 gtcggaccta gtacgttttt gttaaaggcg tcgaaacgca gcgcccagtc gagaatatta
    10141 cgcccaatca gatccggatc gatcttgcct tcacgttcca gtaacgcatc cgccagacac
    10201 agcgccatcg aggtatcgtc ggtgaattcg gcgcggttaa aataacaggc cgcgttattc
    10261 tcctttggtc caggaagaaa acggtcaatc cagccaaagt gtgctttaac gcggctgcgt
    10321 ggccaaagct cggagggcat ccccatcgca tcccctaacg cctgcccata aagagcaccg
    10381 agaatacgtt ctgttttcat ttaacttccc cttgtgtcaa cgcaatatcg cgatcatcga
    10441 ccttgatagc cgtaatttcg ttgtcggatt cgcggaaaaa aatcatgaac agcacggcga
    10501 taatggcaat catcaccgcg ccgaaagtcc acatcccgga ccagttgaaa gtcagtccgt
    10561 ttaccggttc ctgataagcg aacatctttt ccatcatcac accgccaaga cgatagccga
    10621 gcaaactgcc gaagccctgg cagcagagcg tgatcagccc ctgcgcagcg gtacgcatat
    10681 gcacgggggc ttttttatcg acatagatgt aagcggtaac gtagtaaaaa tcgtaactta
    10741 cgccgtgaag caaaataccg aggaacagta acgcgtaggt gaaatattca tccgtactac
    10801 cgtaaataaa gaagccatag cggatcgcag cggtgaccag accaagcaat aatacctttt
    10861 tgataccaaa gcgtttagtg aaaaacggca atgccagcat aaagaagatt tcagagaact
    10921 ggccgagcgt catccagccg gtggcgtttt tcatgccaac ttcggtcaga taaccgttgg
    10981 caaagatgta atagaacgcc agtggcatcg caaacaggaa tgaacagaag aaaaagacga
    11041 ggaagttttt atcgcgcagc aggatcagcg catccaggcc gagcatgact ttaatatcca
    11101 ttttgccggt gctttttggt ggcgtgtcgg gcaggaaaaa cgcaaacaca ccgagcagag
    11161 cagaacttcc ggcggtaatc agcagcggga tgttagtcgg tgagatatcg gcatacccca
    11221 gtatttgcgg caagaaacca catgccagac cggaggcaat ccagccgata gtgcccatca
    11281 cacgaatgcg cgggaaatca cgctccacat ccggcacgtt ggcaaaagcg atgctgttag
    11341 tcagcgcaat ggtcggcata taggttagag agtaagccag cagtaacgga aagaacccgg
    11401 caaaagtggt ctgttgcgca gcgaaataca tcagcagcgc gcctgcgaac atcaataccg
    11461 ccagcacttt ttgcgccgag aaaaagcggt cagtgatgga gccaaccaga attggcgaca
    11521 ggatcgccgc aatggcggta caggcatacg accagccaat ttctccggca ctaaaaccgc
    11581 ttttacttaa ccacaaccac aatggcacaa accacgcgcc ccagataaac cattcaacaa
    11641 acatcatgaa cgacagcttt gctgttgttt tcatctttta attccttcat gacaggtaat
    11701 gtaaggtacg cgatgacaat accacttaac aatacctttt aaataccttt gcggtgaaaa
    11761 cttgaccgcg ctaacatttt ttctgatgaa tcgagccaac agaaaacgct gaaaaaacat
    11821 ccaaaagatg gaaaaactcg cccgtaacga ccaggattat gattgcccgc aaaaggcggg
    11881 ccaggacaat ccatagccga tatccaatcg gaatttacgg gagcatagta atgacagata
    11941 ttgcgcagtt gcttggcaaa gacgccgaca accttttaca gcaccgttgt atgacaattc
    12001 cttctgacca gctttatctc cccggacatg actacgtaga ccgcgtaatg attgacaata
    12061 atcgcccgcc agcggtgtta cgtaatatgc agacgttgta caacaccggg cgtctggctg
    12121 gcacaggata tctttctatt ctgccggttg accagggcgt tgagcactct gccggagctt
    12181 catttgctgc taacccgctc tactttgacc cgaaaaacat tgttgaactg gcgatcgaag
    12241 cgggctgtaa ctgtgtggcg tcaacttacg gcgtgttggc gtcggtatcg cggcgctatg
    12301 cgcatcgcat tccatttctc gtcaaactta atcacaacga gacgctaagt tacccgaata
    12361 cctacgatca aacgctgtat gccagcgtgg agcaggcctt caacatgggc gcggtggcgg
    12421 ttggtacgac tatctatttt ggctcggaag agtcacgtcg ccagattgaa gaaatttctg
    12481 cggcctttga acgcgcgcac gagctgggca tggtgaccgt gctgtgggcc tatttgcgaa
    12541 actccgcctt taagaaagat ggcgttgatt accatgtttc cgccgacctg accggtcagg
    12601 caaaccatct ggcggcaacc atcggtgcag atatcgtcaa acaaaaaatg gcggaaaata
    12661 acggcggcta taaagcaatt aattacggtt acaccgacga tcgtgtttat agcaaattga
    12721 ccagcgaaaa cccgattgat ctggtgcgtt atcagttagc taactgctat atgggccggg
    12781 ctgggttgat aaactccggc ggtgctgcgg gcggtgaaac tgacctcagt gatgcagtgc
    12841 gtactgcggt tatcaacaaa cgcgctggcg gaatggggct gattcttgga cgtaaagcgt
    12901 tcaagaaatc gatggctgac ggcgtgaaac tgattaacgc cgtgcaggac gtttatctcg
    12961 atagcaaaat tactattgcc tgatacgcct catacgaaca gcaccacccc gtaggacgga
    13021 taaggcgttt acactgcatc cggcaaccta tgcctgatgc gacgcggaag cgtcttatca
    13081 tgcctacata gcactgccac gtatgtcgga aaaggcgttt acaccgcatc cggcataaaa
    13141 acacgcgcac tttgctacgg cttccctatc gggaggccgt ttttttgcct ttcactcctc
    13201 gaacagtttt catattgtcg ttttgtgatc gttatctcga catttaaaaa caaatcattt
    13261 cattatattt tgaaatcgaa aacaagcaac aggatatgaa aatgtacgtg gtatcgacaa
    13321 agcagatgct gaacaacgca cagcgcggcg gttatgcggt tccggcattc aatattcaca
    13381 atctcgaaac gatgcaagtg gtggtggaaa ccgctgccaa cctgcatgcg ccggtcatca
    13441 tcgccggaac gcctggcaca ttcacttatg ctggtacaga aaatctgttg gcgctggtca
    13501 gcgcgatggc gaagcaatat caccatccac tggcaattca tctcgaccat cacacgaaat
    13561 ttgacgatat cgctcagaag gttcgttctg gcgtgcgctc agtcatgatt gacgcctcgc
    13621 atttgccttt tgcgcaaaat atttcacggg tcaaagaggt ggtggatttt tgccatcgct
    13681 ttgatgtcag cgtcgaagcg gagctggggc aacttggcgg ccaggaagat gatgtgcaag
    13741 tcaatgaagc cgatgttttt tataccaacc ccgctcaggc gcgtgaattt gccgaggcaa
    13801 ccggaattga ttccctggcg gtcgccatcg gcacggctca tgggatgtat gccagcgcac
    13861 cggcgcttga tttttctcga ctggagaaca ttcgccagtg ggtgaactta ccgctggtgc
    13921 tgcatggcgc gtcagggtta tcgactaagg atattcagca aaccatcaaa ctggggatat
    13981 gcaaaatcaa cgttgcaacg gagctgaaaa atgccttctc gcaggcgtta aaaaattacc
    14041 tgaccgagca ccctgaagcg accgatcccc gggattattt gcagtcggct aaatccgcaa
    14101 tgcgcgatgt ggtgagcaaa gtgattgccg attgtggctg cgagggcagg gcataacgca
    14161 cctgccattt aacaaggaaa aaacatgaaa acgttaattg cccggcataa agctggtgaa
    14221 catatcggca tatgttcagt ctgttctgcc catccgttgg ttatcgaagc ggcgctggca
    14281 tttgatcgca acagcacgcg caaagtgctg attgaagcaa cgtcaaacca ggtcaatcaa
    14341 tttggcggtt ataccggaat gacaccggca gactttcgcg aatttgtttt tacgattgcc
    14401 gataaagtcg gatttgcacg cgaacgcatt attctcggcg gcgatcatct ggggccaaac
    14461 tgctggcagc aagaaaatgc ggatgcggcg atggaaaaat ccgtcgagct ggtaaaagca
    14521 tatgttcgtg ctggcttcag taaaattcat cttgatgcgt caatgtcctg cgcgggggat
    14581 cccataccgt tagcaccaga aacggttgcg gaacgagctg ctttgctttg ctgcggaaag
    14641 tgtggcgaca gattgccagc gtgagcaact gagctatgtc attggcactg aagttccggt
    14701 tccgggcggt gaggccagcg ccattcagtc agtacacatc acccatgttg aagatgccgc
    14761 caatacttta cgtacgcatc aaaaggcctt tattgcccgt gggctgacag aggcgttaac
    14821 acgcgtgatt gccatcgtgg tgcagccggg cgtggaattt gatcacagca atattatcca
    14881 ttatcagccg caggaagcgc agccgctggc gcaatggata gaaaacaccc gaatggttta
    14941 tgaagcacat tctaccgatt accagacccg gacggcttat tgggaattag tccgcgatca
    15001 ctttgcaata ttgaaagtcg gtcccgcatt aacctttgct ttacgcgagg cgatatttgc
    15061 actggcacaa attgagcagg aacttatcgc ccctgaaaat cgcagcggtt gcctggcggt
    15121 aattgaagaa gtgatgctcg acgaaccgca atactggaaa aaatattatc gtacgggttt
    15181 taacgattca ttactggata ttcgttacag cctgtcggat cgtattcgtt attactggcc
    15241 gcatagtcgg attaaaaata gcgtcgaaac gatgatggtg aatcttgaag gcgtggacat
    15301 cccactgggc atgattagtc agtatcttcc caaacaattt gaacgcattc agtccgggga
    15361 attatcagca ataccgcatc agctgattat ggataaaatt tatgatgttt tgcgcgccta
    15421 tcgctacggc tgtgcggaat aaggacggta tatgactaac ctgtttgttc gtagcggaat
    15481 ttcttttgtc gatcgtagcg aagttttaac ccatatcggt aatgagatgc tcgccaaagg
    15541 tgtggttcat gatacctggc cacaggcatt aattgccaga ga
//
LOCUS       DAJVQG010000094        15367 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJVQG010000094 DAJVQG010000000
VERSION     DAJVQG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15367
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.094"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..250)
                     /locus_tag="OQM83_002664"
     CDS             complement(<1..250)
                     /locus_tag="OQM83_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCS1520891.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKL"
     gene            complement(255..440)
                     /locus_tag="OQM83_002665"
     CDS             complement(255..440)
                     /locus_tag="OQM83_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCS1520892.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(437..3076)
                     /locus_tag="OQM83_002666"
     CDS             complement(437..3076)
                     /locus_tag="OQM83_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCS1520893.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3284..4273
                     /gene="ldhA"
                     /locus_tag="OQM83_002667"
     CDS             3284..4273
                     /gene="ldhA"
                     /locus_tag="OQM83_002667"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCS1520894.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4384..4806
                     /gene="hslJ"
                     /locus_tag="OQM83_002668"
     CDS             4384..4806
                     /gene="hslJ"
                     /locus_tag="OQM83_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCS1520895.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4803..5069)
                     /locus_tag="OQM83_002669"
     CDS             complement(4803..5069)
                     /locus_tag="OQM83_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCS1520896.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5343..8867
                     /gene="nifJ"
                     /locus_tag="OQM83_002670"
     CDS             5343..8867
                     /gene="nifJ"
                     /locus_tag="OQM83_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCS1520897.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9233..10366
                     /gene="ompN"
                     /locus_tag="OQM83_002671"
     CDS             9233..10366
                     /gene="ompN"
                     /locus_tag="OQM83_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HCS1520898.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10507..10941
                     /gene="uspF"
                     /locus_tag="OQM83_002672"
     CDS             10507..10941
                     /gene="uspF"
                     /locus_tag="OQM83_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCS1520899.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11527..12441
                     /gene="sitA"
                     /locus_tag="OQM83_002673"
     CDS             11527..12441
                     /gene="sitA"
                     /locus_tag="OQM83_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCS1520900.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            12441..13268
                     /gene="sitB"
                     /locus_tag="OQM83_002674"
     CDS             12441..13268
                     /gene="sitB"
                     /locus_tag="OQM83_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCS1520901.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            13265..14122
                     /gene="sitC"
                     /locus_tag="OQM83_002675"
     CDS             13265..14122
                     /gene="sitC"
                     /locus_tag="OQM83_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCS1520902.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            14119..14976
                     /gene="sitD"
                     /locus_tag="OQM83_002676"
     CDS             14119..14976
                     /gene="sitD"
                     /locus_tag="OQM83_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCS1520903.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(15177..>15367)
                     /locus_tag="OQM83_002677"
     CDS             complement(15177..>15367)
                     /locus_tag="OQM83_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCS1520904.1"
                     /translation="MTWGDADNQQVKQSMPELEELAAAMAQAQVERNDEIYRRQRELK
                     EELNSLKDLNSVRNFIVE"
BASE COUNT         3855 a         3886 c         3704 g         3922 t
ORIGIN      
        1 cgagtttcgc gatatcatct accgacacat tattttgctt tgccagttgt tggtaactcg
       61 ctttacgttc ggcattaatg tcggttacta atttctctgt ttcagcatcc gttttcagcg
      121 caaccagata accataaaat gtttcaccta cccgcccctg agttctggct tcatccagag
      181 tcaatgccat tacattgctg cttaccagcc caacaagaaa cgcacaaaga agtaatgttt
      241 tcttcatcat cgcctcagaa aagatcgcta cgagtttcaa gcagctcttc gacatctttg
      301 tctgccttga tgatgatctc atgctcaatt ttaacgttca tattgatagt gatcggttcc
      361 ttgggcgcag cgacttcaat acgcggcgta cagccaacca gcataaaaga tgacgtcaac
      421 gcagccagta aaattttcat tgtttttcct cacactcttt tccttgcgga cagtcatttc
      481 ccggcagacg tgcgttctgc tccaaccatg cctggagatt atcgccaaag cgtaaactgc
      541 gccacagcgt aaaaatattc tcttcatgat ggtaattaag gtttaccgta ccgcttttac
      601 catcaacgcg actggtacct gtaatgttgg cctgcatggt taataaaccg agattatcta
      661 agttaatttt tgtcgatgac cggctaattt ccatatagcg caaccagtta attgctgaac
      721 ccgcagtcat attgtctttc accaccgcat ctgcggtgtc tttatccagc cgcaaggtca
      781 ttggcccgct atttgccagc caaccatctt tcactatcca tttttcgttg tttagccata
      841 acggcaatgc accactaaat gccccggaca tggcgaattg tttcggattg acggcgctaa
      901 ccagttcgct ggatgagaga ttattcagcc gcaacagcgc cgggtcatgt tgcggcatac
      961 gtaattgttt catcagtacg ttaccgccta acacatcgac gctaacatca gtcaacagca
     1021 agggttcttc ttcagtccac ggataacgcc cttgcaaatc agccgtaata tttttcgctg
     1081 tcaccagatt aatcacttcg gcaattcgca acgtaacggg gccgcgagtc cccagatgcc
     1141 aggctccatc ggcaaaacgg aaaggcagga caaaatcgac accgttaacc tgattatctg
     1201 gcatccaggc actaccgcct ttcaacacgc cgtgtcctcc cgcgcggaat ccttgttcag
     1261 gcgcagcaga aaatgcaacc tgagcataca gttccccatc gcgtaagttc atcttccagt
     1321 cgggtggcac cagcggctgg aatacagtca gtgattgttt tggccaccag gcgttaccgc
     1381 gcagacgaat accgtcccag cgaccattta cccgaaccgg gccaatctca ccagcatgta
     1441 aatcgccttt aaagaggaaa tacgtcggat cgcgcccatc gacgctaaat tttaaggttg
     1501 atggcggtaa cacactgccg ccagtgaaca gcgtttgccc ggcgtccagt gacagcgcgc
     1561 cgctgaagga gggatgctgt gcgtcacgta cccagacgac gggcttgtcg agaattaatc
     1621 gcggcttttc taccgtcatc gtaccgtatt gtaactggtc gaaaccggtg gagaggtcgg
     1681 tcagcgtaat cgtgctgtca tgccattcac cttttcctgc gacatcccag gtggcattta
     1741 tcggtgtaaa actgcctttt ccccagtagc gccactgcca gcggcccgcg tcagggagaa
     1801 aatcattcgc cagcccatcc atatgcagca cgaaatcgcc cagttcatat tcatgcgcct
     1861 gcaaaatggc ctgcaaacgt ccgtcaacac cacgttgggt gacttttaca cccgctaaag
     1921 gccagcggat ttcatcgatg tccagcgaat cgatgactct tcccttcgaa cgtaataacg
     1981 cgcctggctc aaaggccagc gttgggtcag tcagacttcc acttagctgc gcgggtaagc
     2041 gggcatataa aatgaggtcc gcctgtttcg cttcaccggt cagctgcaga ggcagctggc
     2101 tgttatccat gcttaatttt cctgggccaa aatttagcac cgcgttgcct ttacccgctt
     2161 gcccctgggt cagcacactc agtcgtccgc tgatcagagc gttctcaagc cctgcctgcc
     2221 agttgtcgac tttgacaccc agtcggccac tcaaaggaaa acctgcatac ggccaactcc
     2281 agcgaccatc gcttacggtc aattgttgac gagtaatttg ccacggcaaa tcgagcaacg
     2341 gatcgccgtt atcccgtgcc agcacaatca attgcccgct attttcctgc cagtccagct
     2401 ctgcatccac cagtgacggt tcctgcggca agtttaacgt cgcagtagca tgcccactta
     2461 caggaagtcc atccggcacg agcggcatag taaattcccc caccagttta accggcggct
     2521 gattttcaaa cgcgatgaca tccagttcgc tgactgtaag ttgttgccct ttcagctggc
     2581 cttgaaattt aactttttcg ccctgataac gcagttgctg aatatcagag gttaatgcga
     2641 gagagagttt tccctgccat tcctgccagg gagaaaaaat cagtttatcg atattgatcc
     2701 aggtgttagg cagcatggac tgccactgcg cgagggtttt tggagcagct ggcgattgct
     2761 ccgtctgcgg caatttcgcc agacaagcag aatcaagttc taccatgccg acgttcaata
     2821 accagcggct gggatgtgaa aggctggcgt tggtgatatg cgcaagctga caatctccca
     2881 ccagataacg gagatcggga atgattaaac ctttacgcgt aatgcgtggg ctttcatcta
     2941 atgcaatacg tgtcccgagc ggtagccaga tgcccgccag cgtgggaacc cacagcccga
     3001 gcgtcatcag cagcgtcaac ggcacaagaa taatcagtaa taacagcgcg agaacggctt
     3061 tatatttacc cagcatgggt agttaatatc ctgatttagc gaaaaattaa gcattcaata
     3121 cgggtattgt ggcatgttta accgttcagt tgaaggttgc gcctacacta agcattgttg
     3181 ttgatgaatt tttcaatatc gccatagctt tcaattatat ttgaaatttt gtaaaatatt
     3241 tttagtagct taaatgtgat tcaacatcac tggagaaagt cttatgaaac tcgccgttta
     3301 tagcacaaaa cagtacgaca agaagtacct gcaacaggtg aacgagtcct ttggctttga
     3361 gctggaattt tttgactttc tgctgacgga aaaaaccgct aaaactgcca atggctgcga
     3421 agcggtatgt attttcgtaa acgatgacgg cagccgcccg gtgctggaag agctgaaaaa
     3481 gcacggcgtt aaatatatcg ccctgcgctg tgccggtttc aataacgtcg accttgacgc
     3541 ggcaaaagaa ctggggctga aagtagtccg tgttccagcc tatgatccag aggccgttgc
     3601 tgaacacgcc atcggtatga tgatgacgct gaaccgccgt attcaccgcg cgtatcagcg
     3661 tacccgtgac gctaacttct ctctggaagg tctgaccggc tttactatgt atggcaaaac
     3721 ggcaggcgtt atcggtaccg gtaaaatcgg tgtggcgatg ctgcgcattc tgaaaggttt
     3781 tggtatgcgt ctgctggcgt tcgatccgta tccaagtgca gcggcgctgg aactcggtgt
     3841 ggagtatgtc gatctgccaa ccctgttctc tgaatcagac gttatctctc tgcactgccc
     3901 gctgacaccg gaaaactacc atctgttgaa cgaagccgcc ttcgatcaga tgaaaaatgg
     3961 cgtgatgatc gtcaatacca gtcgcggtgc attgattgat tctcaggcag caattgaagc
     4021 gctgaaaaat cagaaaattg ggtcgttggg tatggacgtc tatgagaacg aacgcgatct
     4081 attctttgaa gataaatcca acgacgtgat ccaggatgac gtattccgtc gcctgtctgc
     4141 ctgccacaac gtgctgttta ccgggcacca ggcattcctg acagcagaag ctctgaccag
     4201 tatttctcag actacgctgc aaaacttaag caatctggaa aaaggcgaaa cctgcccgaa
     4261 cgaactggtt taatcttgcc gctcccctgc attccagggg agcttattca gataatcccc
     4321 aaagaccttt catcttctat tcttaaaata gccctgagtc agaaactgta attgagaacc
     4381 acaatgaaga aagtagccgc gtttgttgcg ctaagcctgc tgatggcggg atgtgtaagt
     4441 aatgacaaaa ttgctgttac gccagaacag ctacagcatc atcgctttgt gctggaaagc
     4501 gtaaacggta agcccgtgac cagcgataaa aatccgccag aaatcagctt tggtgaaaaa
     4561 atgatgattt ccggcagcat gtgtaaccgc tttagcggtg aaggcaaact gtctaatggt
     4621 gaactgacag ccaaagggct ggcaatgacc cgtatgatgt gcgctaaccc gcagcttaat
     4681 gaactcgata acaccattag cgaaatgctg aaagaaggtg cacaagtgga tctgaccgcg
     4741 aaccagttaa cgctggcgac cgcgaaacag acattaactt ataagctggc ggatttaatg
     4801 aattaatagc tgccacagct cccggcggca agtgactgtt cgctacagcg tttgccgttg
     4861 ggtaatgcac acatcccaat cgccgtacca tccagttgac gggcaacaga aagcgaaccg
     4921 ccgatcattg cacaatttgc ttctccacta ctggacatcg acgcttttaa acctggcgct
     4981 acgtgcgccg cagtcgcctg ctgaacaggt tcactactac acgccgacaa caataaagcg
     5041 gcacacccta cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca agccaaacga
     5101 caccgccata aataataggc agcacagagg gcggcgtcga gagctgtcct gcgcgttgcc
     5161 ccgcattttt acttttttat ggctattttt ttgccctctg tttgatcaaa acattcatta
     5221 cgctgatgtg ggggacacaa aagcgaaaat gcagaagaaa gccatttgct aaaattgaag
     5281 gattactact gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg taagggtgtc
     5341 atatgattac tattgatggt aatggcgcgg ttgcttcggt ggcgtttcgc accagtgaag
     5401 ttatcgccat ctaccctatt acccccagtt ccacgatggc agaacaggct gatgcctggg
     5461 ccggaaacgg cttgaagaac gtttggggag acacaccatg cgtggttgaa atgcagtcgg
     5521 aagcgggtgc tatcgctacc gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt
     5581 ttacgtcatc gcagggtttg ctgctgatga tcccgacact gtacaaactg gcaggcgaac
     5641 tcacaccgtt tgtcctgcat gtagcggcac gtaccgttgc cacacatgca ctctctattt
     5701 ttggcgatca ttccgacgtc atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg
     5761 caaacgtcca ggaagcgcaa gactttgctc tcatttcgca catcgcgacg ctgaaaagcc
     5821 gcgtgccatt tattcatttc tttgatggtt tccgcacgtc ccacgaaatc aacaaaattg
     5881 tcccgctggc cgatgacacg attcttgatc tcatgccgca ggccgaaatt gatgctcatc
     5941 gcgcccgggc gctcaacccg gaacatccgg tgatccgtgg tacgtccgcc aatcctgaca
     6001 cttatttcca gtctcgcgaa gccaccaacc cctggtacaa cgcagtctat gaccatgttg
     6061 aacaggcgat gaatgatttc tctgccgcga caggtcgtca gtatcagccg tttgaatatt
     6121 acgggcatcc gcaagctgaa cgggtgatta tcctgatggg ctcagccatt ggcacctgtg
     6181 aagaagtggt tgatgaattg ctaacccgtg gcgaaaaagt cggcgtgctg aaagttcgcc
     6241 tgtaccgccc cttctcagcc aaacacttgc tacaaactct accgggatcc gtacgcagcg
     6301 tggcggtact ggacagaacc aaagaacccg gtgcccaggc agaaccgctc tatctggatg
     6361 tgatgaccgc actggcagaa gcctttaata atggcaagcg cgaaactctg ccccgtgtca
     6421 ttggtgggcg ctatggtctt tcatccaaag aatttggccc ggactgtgta ctggcggtat
     6481 ttgccgagct caacgcggct aaaccgaaag cgcgctttac ggttggtatt tacgatgatg
     6541 taaccaatct gtccctgccg ttgccggaaa acacccagcc aaactcggcg aaacttgaag
     6601 ccttgtttta tggccttggt agtgatggca gcgtttccgc gaccaaaaac aatatcaaga
     6661 ttatcggtaa ttccacgccg tggtacgcac agggctattt tgtttacgac tccaaaaaag
     6721 cgggcggcct gacggtttct caccttcgcg tgagcgaaca gccgattcgt tccgcttatc
     6781 tcatttccca ggctgatttt gttggctgcc accagttgca gtttatcgat aaatatcaga
     6841 tggctgagcg tttaaaacct ggcggcattt tcctgctcaa cacgccgtac agcgcagatg
     6901 aagtgtggtc gcgcttgccg caagaagttc aggccgtgtt aaaccagaaa aaagcgcgct
     6961 tctatgtgat taacgcggcg aaaatcgccc gcaaatgtgg cctggcggcc cgtattaata
     7021 ccgtcatgca gatggctttt ttccatctga cgcaaattct gcctggcgat agcgccctcg
     7081 cagaattgca gggtgcgatt gccaaaagtt acagtagcaa aggccaggat ctggtggaac
     7141 gcaactggca ggctctggcg ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg
     7201 taaatccgca cagcgccaat cgaccgccag tggtttccga tgccgcccct gatttcgtga
     7261 aaaccgtaac cgcagcgatg ctcgcagggc ttggtgatgc cctccccgtt tcggcgctgc
     7321 cgccagacgg cacctggccg atgggcacca ctcgctggga aaaacgcaat atcgccgaag
     7381 agatccccat ctggaaagag gaactctgta cccaatgtaa ccactgcgtc gcagcttgcc
     7441 cacactcagc tattcgcgca aaagtggtac cacccgaagc gatggaaaac gcccctgcca
     7501 gcctgcattc gctggatgtg aaatcgcgtg atatgcgcgg gcagaaatat gtcttgcagg
     7561 tggctccgga agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg gcgaaagacc
     7621 gtcagaatcc agagattaaa gccatcaata tgatgtcgcg cctggaacat gtcgaagaag
     7681 agaaaatcaa ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc aaactggaac
     7741 gtattgatat tcgtacatcg cagctgatta caccgctgtt tgaatattcc ggtgcttgct
     7801 ccggttgtgg cgagacgccg tatattaaat tgctgactca gctctatggc gaccggatgt
     7861 tgatcgctaa cgccaccggc tgttcttcaa tttatggcgg taacctgccc tctacaccgt
     7921 ataccaccga tgccaacggt cgtgggccgg catgggcgaa ctctctattt gaagataatg
     7981 ccgaatttgg ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc gtgctgcgtc
     8041 tgctggatca atttgccgat aaaatcccgg cggaattact gacggcgttg aaatcagacg
     8101 ctacgccaga ggttcgtcga gaacaggtcg cagctttacg ccagcaactc aacgatgttg
     8161 ccgaagcaca tgaactgcta cgtgatgcag atgcactggt ggaaaaatca atctggctga
     8221 ttggtggtga tggctgggct tacgatatcg gctttggcgg tctggatcat gtattgagtt
     8281 tgacggaaaa cgtcaacatt ctggtgctgg atacgcaatg ctattccaac accggtggtc
     8341 aggcgtcgaa agcgacaccg ctgggtgcag taactaaatt tggcgagcac ggcaaacgta
     8401 aagcgcgtaa agatcttggc gtcagtatga tgatgtacgg tcatgtttat gtggcgcaga
     8461 tttctctcgg cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg gaagcgtatc
     8521 cgggaccatc gctgatcatt gcttatagcc cgtgtgaaga gcatggttac gatctggcac
     8581 tcagccatga ccagatgcgc caactcacag ctaccggctt ctggccgcta tatcgcttcg
     8641 atcctcgtcg tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc ccgccgtcag
     8701 aagcactgga agaaacgtta cttcacgagc aacgtttccg tcgtctgaat tcgcagcagc
     8761 cagaagtggc agaacagtta tggaaagatg ctgcagctga tttgcaaaaa cgctatgact
     8821 tcctggcaca aatggccgga aaagcggaaa aaagcaacac cgattaaatg ctctggataa
     8881 ggattaccca attctaaaaa aaagcccgga cgactgttcg ggcttgtctt tttatatgtt
     8941 ggaaaatcag tggcaatgca atggcccaac agaaaataaa atctgtgaca ataaaggcat
     9001 ataacccgcg cagaataacg tatagaaaat taaaagtata atttttattt tgtatgtatt
     9061 atcttcgata cattcaccag acttattatc atttcagaag aattatttga ttaaggtttt
     9121 acttaaggcg taacaaatga tatttatcgg ctaactgaac ttctccttta ggatgttttc
     9181 actccccgga atggggataa aaggcaaata aaataactaa aggatttatt caatgaaaag
     9241 caaagtactg gcacttttaa ttcctgccct gctcgccgca ggtgctgcac atgcagccga
     9301 agtttataat aaagacggca acaaattaga tctgtatggc aaagttgatg gcctgcatta
     9361 tttttctgat aattcagcga aagatggcga tcagagctat gcgcgtctgg gttttaaagg
     9421 cgaaacccaa attaacgatc aactcactgg ctacggtcaa tgggaataca atattcaggc
     9481 aaacaacact gaatcttcaa aaaaccagtc atggacccgt ctggcatttg ccgggctgaa
     9541 atttgcagat tacggttctt tcgattacgg acgtaattat ggcgtaatgt acgacatcga
     9601 aggctggacc gatatgctgc ctgaatttgg cggtgactct tataccaatg cagacaactt
     9661 tatgactggt cgtgccaacg gcgtcgcgac ttatcgtaat actgatttct tcggtctggt
     9721 aaatggtctg aacttcgcgg tgcagtatca aggtaacaac gaaggtgcca gtaatggtca
     9781 ggaaggcacc aacaacggac gtgatgttcg ccatgaaaat ggtgacggct ggggtctttc
     9841 cacaacatat gatttaggca tgggctttag cgctggtgcg gcatacacct catctgaccg
     9901 caccaatgac caggttaacc atactgcggc gggtggtgat aaagcagacg cgtggactgc
     9961 tgggctaaaa tacgatgcta acaatattta cctggcaacc atgtattcag aaacgcgtaa
    10021 tatgaccccg tttggcgaca gcgattatgc tgttgcaaac aaaacccaga actttgaagt
    10081 cactgcacag taccagtttg attttggtct gcgtccggca gtctctttcc tgatgtctaa
    10141 aggccgtgac ctgcacgctg ccgatggtgc agacaacccg gcaggtgttg atgataaaga
    10201 tcttgttaaa tacgccgatg ttggcgcgac ttactatttc aataaaaaca tgtccactta
    10261 tgttgactat aaaatcaacc tgttggatga agatgacagt ttctacgctg ccaacggcat
    10321 ctctaccgat gatattgtcg ctttaggtct ggtttatcag ttctaaatcc tcctgcccgc
    10381 tgttatggcg ggctttttct gcttattctt cctcctttga tctaaattaa aaatgtgaac
    10441 tccgtcatta cacaaaaagt gtcatctggc gttacacttt atgcggatac taaaacagga
    10501 ggttttatga acagaacgat tcttgtccct atcgatattt ccgattcaga attaactcaa
    10561 cgcgtgatta gccacgttga ggcagaggca aagattgacg atgcagaggt tcatttcctg
    10621 acggtaatac cttcactgcc ttactatgcc tctctgggtt tagcgtattc cgcagaatta
    10681 ccggcaatgg atgacctgaa agcggaagcc aaatcgcaac tggaagagat cattaaaaaa
    10741 tttaaactgc caaccgacag agtgcatgtc catgttgagg aaggctcgcc caaagaccgc
    10801 attctggaat tggcgaagaa gatccccgct catatgatca tcattgcttc ccatcgaccg
    10861 gatatcacca cttatctgct cggttccaac gccgcagctg tagtgcgtca cgcagagtgc
    10921 tccgtgctgg ttgtgcgctg acactaacgc ccgcacattg ctgcgggctt tttgattcat
    10981 ttcgcaaatg tgctgacatt ttcccctcta atccgtacca tacacgccac agttttatat
    11041 cagatttctt atgctgggcg ttccggcatg acgcatactc ttctgatgcc atataacgaa
    11101 ttgagtcgct tttaaatgtc gcaaaatcaa gaaattagta agaaagaaca atacaacctg
    11161 aacaagtaag ggcaaaaatc acaactatct gacccataag tattttattt atttttcaat
    11221 atgttaaaca ttatcagcga caacaataag ctacgataac ccactctttt tttgcccctt
    11281 ttttgcccct tctacagcgt tttgccccat ttttgccatc aacaattttc ctcataaagt
    11341 catttcaaag cctaatagct gctctacttt tttcctcaat atcatgtcgc cagcaggaaa
    11401 caacctaatc cctgttttag gcctaaaagc tgttaatgat aatcattttc attttgtatg
    11461 ggttatgcta taaaacatag ccgttgctat gtttgttcta attttgctca ctataggtac
    11521 taaattatgc actcaataaa aaaagtaacc atgctcttgg ggtgcctcgc cctcacctgc
    11581 tcgatcgcat ttcaggcaag tgcaactgaa aaattcaagg tcattacaac atttaccatc
    11641 atcgcggata tggccaaaaa cgtggctgga gatgctgcag aagtctcatc cataaccaag
    11701 cctggtgcag aaattcatga gtatcagcct acccctggcg atattaaacg tgcgcagggg
    11761 gcacaactga ttctcgccaa tggtatgaat ctggaattgt ggttccaacg cttttaccag
    11821 catctcaatg gggttccaga agtaattgtc tcttcgggtg tgacgccagt agggatcacc
    11881 gaaggaccct atgagggcaa acctaatccc catgcatgga tgtcgccaga taatgctctg
    11941 atttacgtcg ataatattcg tgatgcgttg ataaaatacg atccggcaaa tgcacaaacc
    12001 taccaacgta atgccgatac ttataaagcc aagattaccc aaacccttgc ccccctgcgt
    12061 aagcagatta cggaactccc tgagaatcag cgatggatgg tcaccagtga aggggctttt
    12121 tcttatctcg cacgcgattt ggggctaaaa gagctttatc tgtggccgat taatgccgat
    12181 caacaaggaa caccgcagca ggtacgtaag gttgttgata tagttaagaa aaatcatatc
    12241 ccggcagtct ttagcgagag tacgatttcc gataaaccag cgcgtcaggt tgcgcgtgaa
    12301 accggcgcac actacggcgg tgtgctctat gtcgattccc tgagcacaga aaacggcccg
    12361 gtacctacgt atatcgacct tcttaaggtt actaccagta cgctggtaca gggaattaaa
    12421 gccgggaaaa gggagaaata atgatgcaat ctgcaggcat tgtcgttaat gatgttactg
    12481 tcacctggcg taacgggcac acagcactgc gcgatgcatc cttcacagta ccaagtggat
    12541 ctattgccgc tctggttggg gtaaacggtt ccggaaaatc gacgttgttt aaagcgatta
    12601 tgggatttgt gcgtctgacc agcggaaaga tatctgttct gggtattccc acgcgacagg
    12661 cgctacagaa aaacctggtt gcctacgtcc ctcagtcaga agaggttgac tggtcatttc
    12721 ctgtactggt ggaagatgtg gtgatgatgg gccgctatgg gcatatgggt atgttgcgta
    12781 tagccaaaaa gagggatcgt cagattgtca ccgatgcgct ggaacgtgtc gatatggtgg
    12841 attttcgcca tcgtcaaatc ggcgagcttt ccggtggaca aaagaaacgc gtctttctgg
    12901 cgagagcgat tgcacagcag ggtgatgtga tcctgctgga tgaacctttt accggcgttg
    12961 atgtgaaaac cgaagcgaaa atcatcagcc tgttgaggga gttacgcgcg gaaggcaaaa
    13021 cgatgcttgt ctcaacgcac aatctcggct cagtcacgac attttgcgat tatacggtca
    13081 tggtcaaagg caccgtcctg gcaagcgggc cgacagacac cacttttaca gccgagaacc
    13141 tggagctggc ttttagcggc gtactgcgcc atgtcacact taacgactcg gaagaaagca
    13201 ttattactga cgatgaacgc ccttttgtgg cacatcgacc gtcagcggtg cagagggaag
    13261 caagatgaac gtgctactgg aacccttcag ctatgaatat atgctcaatg cgatgtgggt
    13321 ctcggcaatg gtcggcggtc tctgcgcttt cctgtcatgc tatctgatgc taaaaggctg
    13381 gtcgctgatt ggcgatgcgc tgtcgcactc tattgtcccg ggtgttgcgg gggcatatat
    13441 gctggggctt ccgttttctc tgggagcctt cttttccggc ggactggcag cgggcagcat
    13501 gctgtttctt aaccaacgca cccgcttaaa agaagatgcc atcatcggcc tgatcttctc
    13561 gtcctttttt ggcctggggc tgtttatggt gtcattgaac ccgacatccg tgaacattca
    13621 gaccatcgtc ctgggcaata ttctggcaat cgatccggcg gatatcctgc aactcactat
    13681 cattggcatt ctctcgatca tcgtgctttt tttcaaatgg aaagatctta tggtgacctt
    13741 tttcgacgag aatcacgccc gtgctattgg attacatccg ggcagattaa aaatcctctt
    13801 cttcaccctg ctgtcggttt cgaccgttgc ggcgctgcaa acggttggtg cctttctggt
    13861 gatctgtctg gtggtgactc ccggtgcaac agcatggttg ttaaccgatc gttttccccg
    13921 cctgctcata attgctgtaa ctattggcag tgtaaccagt ttcctcggcg cgtgggtcag
    13981 ttactttctg gacggcgcga caggtggaat catcgtggtg gcgcagacac tgctgtttct
    14041 tttagcattt gtatttgcgc ccacccatgg acttttggca aatcgtcggc gtgcgcataa
    14101 agcgctggag gatcgctcat gatggcatta ttactggagc cactgcagtt cacctttatg
    14161 agtcacgcac tgctgatttc actggtagtt tccataccct gtgcgctgct gtcggtcttt
    14221 ctggtcctga aaggttgggc actgatgggt gacgcgatga gtcatgcggt ttttcctggc
    14281 atcgtgctta cctggattct ggggttacct ctggctacag gggcattcgt cgccggagta
    14341 ttctgcgcgg tggcgacggg atacctgaaa gacaacagcc gaatcaagca agatacagtt
    14401 atggggattg ttttttctgg catgtttgcc gcaggtctga tcttgtatat tgcaatcaaa
    14461 ccagacgtac atcttgacca tattcttttc ggcgacatgc tcgggataac catcggcgat
    14521 ataatccaga cggtgattat tgccgggctg gttacacttg ttattagcgc aaaatggcgg
    14581 gattttttgc tgttcagctt tgattatcaa caggcgcagg taagcggttt gcatacgaga
    14641 tggctgcatt atgggctgct gtgcatggtt tccctgacca ttgtggcgac gctgaaagca
    14701 gtaggtatca ttctctctat ttctctgctc attgcgcctg gcgccattgc agtactcctg
    14761 acacaacgct ttcatattgc gctcctactg gcgactggca tatcggtaat agtgtcaatg
    14821 actggtgtct ggctgtcctt ttttatcgac agcgcaccag ctccgacgat tgtcgtctta
    14881 ttcgcggtcg tgttcatcat gacgtttacc gttaccagca tcaacgcacg cacaaaggga
    14941 aacgcccata cacaggatct gttatcaccc aattagcaca acatccgagg acagcatcgt
    15001 ccgccagcct acagaagtcc ggaaagccat ggaaggtcgt tgggaggagg tacgaattct
    15061 atcatgcaaa aaatacgtaa aatcgataac gcctagaaat cattcaatac tcgcactatc
    15121 gaaagtttac cagccaaccg cggcacgttc ctgcatacga cgtgccgcag cttctgttat
    15181 tccacgataa aattcctaac cgaattcaaa tccttcagac tattcagctc ttccttcagc
    15241 tcccgctgac ggcgataaat ctcgtcattg cgctcaacct gtgcctgagc cattgctgcc
    15301 gccagttctt ccagttccgg catcgactgt ttcacctgct gattatcggc atcaccccac
    15361 gtcatca
//
LOCUS       DAJVQG010000095        15293 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJVQG010000095 DAJVQG010000000
VERSION     DAJVQG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15293
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.095"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..453)
                     /gene="spy"
                     /locus_tag="OQM83_002678"
     CDS             complement(<1..453)
                     /gene="spy"
                     /locus_tag="OQM83_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCS1520905.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
     gene            complement(783..1751)
                     /gene="astE"
                     /locus_tag="OQM83_002679"
     CDS             complement(783..1751)
                     /gene="astE"
                     /locus_tag="OQM83_002679"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCS1520906.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1744..3087)
                     /gene="astB"
                     /locus_tag="OQM83_002680"
     CDS             complement(1744..3087)
                     /gene="astB"
                     /locus_tag="OQM83_002680"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCS1520907.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(3084..4562)
                     /gene="astD"
                     /locus_tag="OQM83_002681"
                     /pseudo
     CDS             complement(3084..4562)
                     /gene="astD"
                     /locus_tag="OQM83_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4559..5593)
                     /gene="astA"
                     /locus_tag="OQM83_002682"
     CDS             complement(4559..5593)
                     /gene="astA"
                     /locus_tag="OQM83_002682"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCS1520908.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5590..6810)
                     /gene="astC"
                     /locus_tag="OQM83_002683"
     CDS             complement(5590..6810)
                     /gene="astC"
                     /locus_tag="OQM83_002683"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HCS1520909.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            7256..8062
                     /gene="xthA"
                     /locus_tag="OQM83_002684"
     CDS             7256..8062
                     /gene="xthA"
                     /locus_tag="OQM83_002684"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCS1520910.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            8229..8939
                     /locus_tag="OQM83_002685"
     CDS             8229..8939
                     /locus_tag="OQM83_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCS1520911.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8944..9621
                     /gene="ydjY"
                     /locus_tag="OQM83_002686"
     CDS             8944..9621
                     /gene="ydjY"
                     /locus_tag="OQM83_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCS1520912.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9636..10343
                     /locus_tag="OQM83_002687"
     CDS             9636..10343
                     /locus_tag="OQM83_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCS1520913.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            10343..10891
                     /locus_tag="OQM83_002688"
     CDS             10343..10891
                     /locus_tag="OQM83_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCS1520914.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQNPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10901..12067
                     /locus_tag="OQM83_002689"
     CDS             10901..12067
                     /locus_tag="OQM83_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1520915.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            12040..13576
                     /locus_tag="OQM83_002690"
                     /pseudo
     CDS             12040..13576
                     /locus_tag="OQM83_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            13576..14229
                     /locus_tag="OQM83_002691"
     CDS             13576..14229
                     /locus_tag="OQM83_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCS1520916.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            14296..>15293
                     /gene="ynjE"
                     /locus_tag="OQM83_002692"
     CDS             14296..>15293
                     /gene="ynjE"
                     /locus_tag="OQM83_002692"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCS1520917.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKLPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKG"
BASE COUNT         3507 a         4162 c         3992 g         3632 t
ORIGIN      
        1 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
       61 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
      121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
      181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
      241 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
      301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
      361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
      421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
      481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
      541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
      601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attacaacac aatgattttg
      661 cagagattat gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt
      721 aatctgtatt aatttataag aaagcaactt aatacccgca gaatgatttc tgcgggtaag
      781 tattagctta ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caacggatta
      841 gggaataaca catactctac atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc
      901 agcaatgttc ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg
      961 aaggacggcg agtggcgagt aatttgcgaa accacccgat aacggagcgg cggtgttctc
     1021 acgataccga cactctcacc agatagcagc gcagcaattg cgctggcagt tactgcaaac
     1081 tggcgaagat cgttttgccc aaagggcaac gctttgccaa gttccagcgt acaggccagc
     1141 gcgccaaaat gtctggcgct gaaatgggta aacgtaccac caggctcctg atggaacacc
     1201 agcgcctcca gccccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg
     1261 cgttgcggta atacaccgaa ctgaggatgc aaggagccac gaattgcggt atgtagatca
     1321 aggtgccaac gcacagattc tttgccctgg tcataaaaat cttccaggca ctgttccagt
     1381 tcgcgcgccc gacaggtttc tccgctttca gcaaatagct gccaacgacc gccaaacatt
     1441 cggttcatat cgctatggca ataacgtttc ccttgcttca gcgcaggagg atccccgagg
     1501 atcaccagca accgccaacg taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg
     1561 tccaacattt ccacaggtgc cgtctcatta ccgtggatcc ccgcagaaat caccagcgcg
     1621 ccttgcggtg gcgttaatgg cgtcagttcc agcacaccat cgcccagcca gcgccagcga
     1681 acgccgttga tttctcgctc ggtgataacc ggttttttac ccgttaaggt cagagcaaga
     1741 aaattatcca ttgccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa
     1801 ttggctcaat acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc
     1861 ggcagcagta aggcgatcgc ggtagtaacg atccacccag tcattgagcg cattaaacag
     1921 cgtatcgttc atcatcaccg ccggattcac cgcccggcgt tcttcttctg tcaatactac
     1981 ccgcaaccgc aggcacgccg ggccgccgcc attcgccatg ctttcacgta aatcaaagac
     2041 ttttagttcg ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc
     2101 ggcgtgttcc cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcaa
     2161 cagttggctg ttaaacagat aggtagacac cgcatccgac acggaaacct gagttgccgg
     2221 aacttctatc gccataaaac cgttcacccg cgcccgcagg tttgccagta actgtgactg
     2281 gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg ttacttacgg caatcacgtc
     2341 attatgaaaa acaccctggt cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg
     2401 gggattcacc tgattaagcc ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg
     2461 ataacgggaa ggccgggtat cattgccttc ttctcgcccg tagacaaaaa gttgcatacc
     2521 cggctcaccg taatgaccgc cgagacgatt gtggtttgcc gccccctcat caccgagcaa
     2581 cgctacctgc ggcaacgccg aatggacgct aaatttctct tcgtcgttaa aaatagcttt
     2641 taacagcgat tcagtgacgg gcgcttccag cgaacggtga aatttattgt tcaggttggc
     2701 aacggtgaga tgcactttac catccagcgt atcggcagat ggcgcgatcg ttgccgcatt
     2761 ggctacccac attggcgaag cggagctgac gctggaaagc cagtgcggtg cctggcgtgc
     2821 aactttttcc agtacctgct catcgctacc actgaatccc aactgacgca gcaccggaat
     2881 aaacggacgc tcgtgcggcg ggatcacggc ctgggggaat cccgcatcgg caagggtttt
     2941 cattttcagt aagccctgtt tcgccgccag tcgcgggtta gacacctgaa aacggtgacg
     3001 ggtagaggct tcattaccaa acgacaggcc agcgtaatga tgcgtcagcc ctaccagccc
     3061 gtcgaaatta acttcccagg cgttcatcgc accacctcat cggaaaaatc cagcccgggg
     3121 ttaagcgttg cgggcaaagt taacgagtcc gactccaggc tcgccatagg ccatgcgcag
     3181 taatctgcgg cataccaggc gctggggcga tggttaccgg atgcaccaat gccgccgaat
     3241 ggcgcggtac tggcagcacc agtaagcggt ttgttccagt taacaatccc cacccgcgcc
     3301 tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa ccagaccgca agagagtccg
     3361 aagcgagtgt tattcgccat tagaatcgct tcctcgaaag aatcataacg ccagacgcgc
     3421 aataacggtc cgaacacctc ttcatctggt acgccagcaa cgcctgtcat ttcaatgatc
     3481 cccggcgtca gcaacgatgt ctctgattgt aataagcgcg gcgcaagcag ggttcgtccg
     3541 cccatcgctt ccagttgctg ctaggcagta accacctgct gtgcggcctg ttcagaaatc
     3601 agcccgccaa taaacggctg cggttcgtca tcccagttgc ccggcgttaa tcgctggctg
     3661 acggcaacca gacgagcaag aaacgcatcg ccctgtgctc cgcttttgag caataaacgg
     3721 cgggcgcagg tgcagcgttg cccggctgtg acaaacgccg actgaatggt cagatggaca
     3781 gccgagtcga tatccgccac ctcatcgata attagcggat tattaccgcc catctcaagg
     3841 gcgagaattt tctccggctg accggagagc tggcgatgca gctggtagcc ggtattggcg
     3901 ctaccggtaa acagcaaacc gtcgagatcc tccagcgcac tcagcgcctg acccgtttca
     3961 cgcccgccct gcaccaggtt cagcacgccc ggcggcaagc cagcctgctg ccataaacgc
     4021 attaccgctt cgccactcca cggtgtcagt tcgctgggtt taaagataat ggtgttacct
     4081 gccagcaatg ccggaacgat atgtccgttc ggcaaatgac cagggaaatt atacggccca
     4141 aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca
     4201 ctacgctgct cgccggtacg aacgtgatac gccttaattg atatcgcgat tttattgatc
     4261 atcgccgtca cttcggttgc cgcttcccag cgcggcttac ccgtttctct ggcaataatc
     4321 gcggttaatt cggctttatt gctttccagc aatccggcaa agcgttcgac aacgacctga
     4381 cgttcagcca atgagagccg cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc
     4441 tgttcgacct gagcggcatc ggcatcattg ccttgccata acacctcgcc cgataccgga
     4501 ttacgcttca cacgcgatgc gccctggccc gttatccagt caccgttaat ccataaagtc
     4561 atgctgtttt ctcctctgcg cacaggcgca ccagacgaac gcgatcccct gcgtggcatt
     4621 tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg
     4681 ccagcaccac gcggaaatgg tgataatttt cattggcgac caggcaggcc ggaaaatcgc
     4741 cctgcgcagg ctgcccttct gccacttcca ccagccgact tttacggatg gcgcgcacgc
     4801 ggtcgatgtc acactcaagc gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac
     4861 gaaaaccttc tttctccagc accgcgcggg caggcgcggt ttgcggatgt acctgaccga
     4921 tgacgtcctg ggcttcctgg gataaaaagt gggtatagat cggatgtttc ggcatcagtt
     4981 ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc gacgcggcta aaatccatcg
     5041 aaaagaagcg tttaccgagg ctttgccaga acggtgaata gccgtgttcg tcaatcaccc
     5101 cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa
     5161 agcgcgattt cgacagcaaa tagccgttgc cctctttgcg ccagtccggg tcgagaaaca
     5221 gcgtgcacag ctcgctgctg ccggtgtgat cgttactaag aaacagcgtc ggcaatgcgt
     5281 tatagacatt cagctctttt gaggcgtgaa ccaacgtgcc gacccgatag ttgtaccagg
     5341 gatcgttcag cccaaccgcc acctcaatgg cgcaaatccc cgccacggtg cctgtctcgc
     5401 tatcttccag cacgaacaca tagccctgct cgcttttggg cagttcgcct tgccaggttt
     5461 tgattgccct ttcgatacgc gcggaaagcg tggcttcatt ggcgggaagc gacgtcaggc
     5521 cgccgcccgt tttgctggca agctgcatca gcgccgagac atctgagcgc tcaacgggac
     5581 ggatgaccat catgatgaac ctcggctaac aaagtgttcg caagcagctg caaagcgatc
     5641 cagtccggtc gtcacctctt cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt
     5701 gccacccgca atcagtacca taacgcctgc tttcgccgcc tcctgagaga tctgtttcgc
     5761 ttgtccggcg taatcggcat tcagtacaca gccaatcaac aaacctaagc cgcgaacttc
     5821 actgaacaaa ccgtagcggt gattaatggt attaagacgc tcaacaaacc agtcgtgacg
     5881 ctgtttaacg ccattaagca tctctggtgt gttgatgagc tccagcactt tgcccgccac
     5941 cgccgaggcc agcggattgc cgccataggt ggtgccatga gtgccaacgg tcatcacgct
     6001 ggcgcactct tcggttgtta acaacgcacc gaccgggaaa ccgccgccca gcgctttggc
     6061 ggtagttaac agatcaggtg tcacgccgta gtgcatatag gcatacagtt ccccggtgcg
     6121 cccgacgccg gtttgtactt catcaaaaat caacagcgca ttgtggcggt cacacaattc
     6181 acgcagacct tgtaaaaacg cgttgctggc tggcaccaca ccgccttccc cctggatggg
     6241 ttcgacaatc accgcacagg tggcgtcgtc aatcagcgcg ctggcagagt taatatcgtt
     6301 atatgcagca tgacggatat ctggtggcag tggcgcaaaa tcctgtgaat aggctggctg
     6361 cccacccgca ctgacagtaa acagcgtgcg accatgaaac gcatttttaa acgccacgat
     6421 accgctctta tggctgccgt agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc
     6481 ttcgttggct tccgcaccgg agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat
     6541 caattttttc gccagtcgca gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa
     6601 cttactcgcc tgttcgttca gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt
     6661 caccgcaatg ccacccgcga agtcgatata ctctttcccc tgctgatccc acaagcgcga
     6721 accttcgcca cgtaccggta taaagggtgc cggagcgtaa acaggtatca tccattcatc
     6781 aaagttttca cgcgtaattg gctgagacat agcgacctct acagtaaata attcgctatc
     6841 tatatgttaa caataagtaa tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc
     6901 cagtgataaa agtgcataaa cggcggaggc taactggaaa tcaaggagtt ataaccaaac
     6961 catatgcatt taaagtgcat ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa
     7021 taaaaaacgc aatgttatgc agaagtaaaa tataattctg gaattgtgat cattgacgaa
     7081 atttactgga aattactgcg ccattctgac gcagcgcgca ccaaaagcgg gcattttttg
     7141 cgccatcgtt gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtaagca
     7201 acgcgaaatt ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa
     7261 atttgtctct tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt
     7321 cgaaaagcac caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt
     7381 tccgctcgaa gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca
     7441 ttatggcgtg gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg
     7501 tgacgacgaa gaggcgcagc ggcggattat tatggcggaa atcccctcac tgctgggtaa
     7561 tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt
     7621 cccggcaaaa gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaaacg
     7681 tgataatccg gtactgatta tgggcgatat gaatatcagc cctacagatc tggatatcgg
     7741 cattggcgaa gaaaaccgta agcgctggct gcgtaccggt aaatgctctt tcctgccgga
     7801 agagcgcgaa tggatggaca ggctgatgag ctgggggttg gtcgatacct tccgccatgc
     7861 taatccgcaa acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga
     7921 taaccgtggt ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt
     7981 agaaaccggc atcgactatg aaattcgcag catggaaaaa ccgtccgatc acgcccccgt
     8041 ctgggcgacc ttccgccgct aatttagcag ctctcctggc tcaaactggg tcaggagaat
     8101 taaccttgag aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta
     8161 taccgtcact gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa
     8221 actccacgat gaatgctgag cgtaaatttc ttttcgcctg tcttattttt gcgctggtca
     8281 tttacgctat ccacgctttc ggtttattcg atctgctcac cgatttaccc cacttacaga
     8341 cactcattcg ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcattg
     8401 ccaccctctt tgtgttacca ggaagcatac tggtgatcgc tggaggaata gtttttggcc
     8461 cgctcttagg gacactactc tcattaattg ccgccacgct ggcctcctcg tgctcattcc
     8521 tgctggcgcg gtggctggga cgtgatttac tgctgaaata cgtgggccat agccatacct
     8581 tccaggccat tgaaaaaggc attgcgcgta acggtattga ttttcttatt ctgacccgct
     8641 taatcccgtt gtttccttac aatattcaaa attacgctta cggattaacc accatcgcct
     8701 tctggcctta tacccttatt tcggcactca cgaccctgcc cggtattgtt atttataccg
     8761 tgatggcaag cgatctcgcc aatgaaggca ttacgctgcg ctttatttta caactctgtc
     8821 tggcgggcct ggcgctgttt attctcgtcc agctcgcaaa actctacgcc cgacacaaac
     8881 atgtggatct gtctgcttcg cgccgcagcc cgcttactca cccaaaaaat gaaggataga
     8941 acgatgtcgc aacattattc agtgtcatgg aaaaaaggac tggctgcact ctgcttactg
     9001 gctgttgccg ggcttagcgg ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac
     9061 gacggacttt cgaacagcca accgctgcgc gtcgatgcca ataaccatac ggtaaccatg
     9121 ttggtgcaaa ttaatggtcg tttcctcacc gacgacactc gtcacggtat tgtgtttaaa
     9181 gatggctcca acggacataa atcgctgttt atggcttatg cgaccccgaa agcattttat
     9241 gaagccctga aagaggcagg tggtacgccg ggcgaaaaca tgacgatgga taataaagaa
     9301 acgactcatg tcacaggcag caaactggat atttcggtca actggcaagg ggcggcaaaa
     9361 gcgtattcct tcgatgaagt gattgttgac agtaatggca agaaactgga catgcgcttt
     9421 ggcggtaatt taacggcagc agaagagaag aaaacgggtt gcctggtgtg tctggatagc
     9481 tgcccggtcg gcatcgtcag caatgcaaca tacacttatg gtgcggtgga aaaacgcggc
     9541 gaagtgaaat tcaaaggcaa tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt
     9601 acctttaaaa tcaccgaata aagccaggat aaaggatgat gaagatgcaa tcgcgtaaaa
     9661 tctggtacta ccgtataacc ctcatcatcc tgttgttcgc tatgctgctc gcatgggcgc
     9721 tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc
     9781 aacagggtat agaacgcttt attcagtctt acggcgcact ggcggcagtt gtctcgttct
     9841 tgttgatgat tttacaggcc attgctgcac cgctacctgc gtttttgatc acctttgcca
     9901 atgcgtcgct gtttggcgcg ttctggggcg gcttgctgtc gtggaccagt tcgatggccg
     9961 gcgcggcgct gtgctttttt atcgccagag tgatgggccg cgaagtggtg gaaaaattaa
    10021 ccggcaaaac cgtgcttgac agtatggacg gctttttcac tcgctacggc aaacacacca
    10081 tcctggtctg tcggttattg ccttttgtcc ctttcgatcc aatcagctat gctgccgatc
    10141 tgacttcaat acgttttcgc tcgtttttta tcgccaccgg gcttggtcaa ttaccgacga
    10201 ctattgttta ttcctgggcg ggcagcatgt taacaggcgg tactttctgg tttgtcaccg
    10261 gactgtttat tctgtttgcc ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg
    10321 agcgccagaa gaggaatgac tgatgggttt accgccgctt agcaaaattc ctttcatttt
    10381 acgtccgcag gcgtggctgc atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg
    10441 gtgggggcgg atcccgttta tcttttattt ggtgtcaatg tttgttgggt ggctggagcg
    10501 caaacgctca ccgctcgatc cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat
    10561 gtgcctgtgt gagttttgtg tggatatcac cagtatgaaa gtcgccgagc gtaccggcag
    10621 cagcgataaa ctgctggcag tggctgactg gcggcaaaac ccgctcttta gcgatgaaga
    10681 acggctggcg ctggagtacg ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc
    10741 cctgcgtacc cgactggctg cgcattttga cgctcaggcg ctcaccgaac tgacggcatt
    10801 gatcggcctg caaaacctgt cggctcgttt taattctgcc atggacattc ccgctcaggg
    10861 gctgtgccgt attccggaaa aacgttctta aggagagaag atgcgccatt gtgggtggtt
    10921 gctgggattg ttatcgctgt tttctttggc aacacatgcc agtgactggc aggaaattaa
    10981 aaatgaggcc aaagggcaaa ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa
    11041 ccgctatctc gactgggtta gcggcgagat gaaaacccat tacgctataa acctgaagat
    11101 tgttcgtctc gcggatgccg cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg
    11161 tcgtaaaacg ggcggctcgg tggatctgct ctgggtgaac ggcgaaaact tccgcacctt
    11221 aaaagaggcc aatttattac aaacgggctg ggcggagact ctgcccaact ggcgctatgt
    11281 cgacacacag ctgccggtgc gggaagattt ttcagtgccg acacaaggtg cggaatcgcc
    11341 ctggggcggc gcacaactga cgtttatcgc ccgccgcgat gttacgccac agccaccaca
    11401 aacgccgcaa gccttactgg agtttgctaa agccaatccc ggcacggtta cttatccgcg
    11461 cccaccggac tttaccggca cggcgtttct tgaacagttg ctgattatgc tgacgccaga
    11521 tcccgccgca ttaaaagaag cgccggacga tgcgactttc gcccgtgtca ctgcgccctt
    11581 gtggcaatat cttgatgcac tgcatccgta tttgtggcgc gaaggaaagg atttcccgcc
    11641 ttcgcccgcg cggatggatg ctctgctgaa agccggcaca ttgcgcctgt cgctgacctt
    11701 taaccccgcg catgcgcagc aaaaaatcgc cagcggcgat ttgccggcaa gcagttacag
    11761 ttttggcttt cgcgagggga tgattggcaa cgtgcatttc gtcaccattc cagccaacgc
    11821 gaatgccagt gctgcggcga aggtagttgc caatttcctg ctctcacccg atgcgcaact
    11881 gcgtaaagca gatcccgttg tctggggcga tccttctgtt ctcgatccgc aaaaactgcc
    11941 tgacgggcag cgcgaaatat tgcaatcaag aatgccgcag gatctgccgc cggtactggc
    12001 tgaaccgcac gcaggatggg tgaatgcact ggaacaagaa tggttacgcc gttacggtac
    12061 gcattaatct ttttgctgtg ggcgatggtg gcggtgattt atgcaccgct gatcccggca
    12121 gctctcacgc tgatatcgcc tgcgttgtcg ttgacacact ggcaggcgtt atttgccgat
    12181 ccacagttac cgcatgcatt actggcaacg ctggtatcga caaccatcgc ggcggtcggg
    12241 gcattgttga ttgccctgct ggtgattgtg gcgctatagc ctgggccgaa atggcagcgt
    12301 atgtgcgccc gtctgccgtg gctgctcgcc attccccatg tggcttttgc caccagcgcc
    12361 ctgctgctct ttgctgacgg agggctgcta tatgactatt tcccgtattt cactccgcca
    12421 atggaccgat ttggcatcgg tctgggcctc acccttgcag tgaaagaaag cgcatttctg
    12481 ctgtggatct tagcggcagt attgagcgaa aaacggctgt tgcagcaggt cattgtgctg
    12541 gattcgctgg gctacagccg ctggcaatgc ctgaactggc tgctgttgcc ctccgtcgcg
    12601 ccttcgctgg caatggcgat gctggcgatt gttgcctggt cgctttcggt cgtggatgtg
    12661 gcgattattc ttgggccagg taatccaccg acgctggcag taattagctg gcagtggtta
    12721 actcagggcg acgcggatca acaaacgaaa ggcgcactcg ccagtctgct gctgatgctg
    12781 ttactcgccg cctacgtttt gctgagctat ctgctatggc gcagctggcg gcgcactatt
    12841 ccccgcgtag atggcattcg caatgcctgc cacaccttta ttaccaggca ctacgctggc
    12901 gagtttttta cccttaaccg gtgtgctgtg tgtggttctg ctggcgatcc tcgcggatca
    12961 gtcgacgatc aatagtgaag cactcatcaa cagtctgaca atggggctgg tagcaacatt
    13021 catcgctttg ctcctgttac tgctgtggct ggaatggggg ccacagcgtc gccagttgtg
    13081 gctatggttg cccattctat tgcccgatct gccactggtg gcgggccagt acacgctggc
    13141 gctatggctg aacctggatg gaagctggac ggcggtggtc tgggggcatc tgctgtgggt
    13201 aatgccgtgg atgctgttta tcctgcaacc cgcctggcag cgcattgatt cacggttaat
    13261 tttgattgcg caaacactgg gctggtcgcg ggccaaaatc ttcttttacg tgaaatgccc
    13321 actcatgttg cgccctgtgc tgattgcctt cgcggtggga ttttcagtca gtattgcgca
    13381 gtatatgccg acgctgtggc tgggcgcggg gcgttttccg acgctcacca ctgaggcggt
    13441 ggcattaagc agcggcggca gcaacggtat tctcgccgcc caggctttat ggcaactgtt
    13501 gttaccgctt attatttttg ccctgaccgc gttagtcgca aaatgggtag gttatgtcag
    13561 acaaggactc cgctaatgct ctgcgtgaaa aatgtttcgc tacgtttacc agaaagccgc
    13621 ttgctgacaa acgttaactt tacggttgat aaaggtgaca tcgtcacgtt aatggggccg
    13681 tctggctgtg gaaaatccac tctgttttca tggatgattg gtgcactggc tggacagttt
    13741 tcttgtacag gtgagctatg gctcaatgag caacggattg acatgctacc caccgcacag
    13801 cgtcagattg gcattctttt ccaggatgca ctgttatttg accagttcag cgtcgggcaa
    13861 aatttactgc tggcgctact gtcgacgctc aaggggactg ccaggcgcaa tgccgtgaaa
    13921 gacgcgcttg atcgagcggg actggctgaa acttatcacc aggaccctgc gacattatca
    13981 ggcggtcagc gagcgcgggt ggccctgctg cgcgcccttc tcgctcagcc aaaagcgtta
    14041 ctcctggatg agccattcag ccgtcttgat gtggctctgc gcgataattt tcgccagtgg
    14101 gtgttcagcg aagttcgcga actggcgatc cccgtcgttc aggtaacgca cgatctccag
    14161 gatgttcctg ctgatagttc tgttctggat atggcgcagt ggtcagaaaa ttacaacaaa
    14221 ctgcgataac gcaaagtttt tctcaatgcg tcagttcaga atggcgcact caaaactaca
    14281 atgtcgggat tttcgatgaa acgtgtttct caaatgaccg cgctggcaat ggctttaggg
    14341 ctggcttgcg cttcttcgtg ggccgctgaa ctggcgaagc ctcttacact tgaccagctt
    14401 caacaacaaa atggcaaagc gatagatact cgccccagcg cgttttataa cggctggcca
    14461 caaaccttaa atggcccttc tggtcatgaa cctgccgcct taaacctctc tgccagctgg
    14521 ctcgacaaaa tgagcaccga acagctcaac gcgtggatca agcaacataa cctgaaagcc
    14581 gatgccccgg tggcgctgta cggtaatgac aaagatgtcg acgccgtcaa aacgcgactg
    14641 caaaaagcag gttttacgca tatctccatt ctgagtgacg cgctaagcga accttcccgt
    14701 ctacaaaaac tgccgcactt tgagcagctg gtttatccgc aatggctgca cgacctgcaa
    14761 caaggtaaag aggttacggc gaaacctgcc ggtgactgga aagtcattga agcggcctgg
    14821 ggcgctccta agctttacct tatcagccat attcccggtg ctgactacat cgataccaac
    14881 gaagtggaaa gcgaaccgct gtggaacaaa gtttctgatg aacaactaaa agcgatgctg
    14941 gcaaaacacg gcattcgcca tgacaccacg gtcattctgt acgggcgtga cgtatacgct
    15001 ggagcgcgtg tggcgcaaat tatgctctac gcaggcgtga aagatgtgcg tctgctggat
    15061 ggcggctggc aaacctggtc cgacgcggga ctgcctgttg agcgaggaac gccaccgaaa
    15121 gtgaaagcgg aaccggattt cggcgtgaag ctcccggcac aaccgcagtt gatgctcgat
    15181 atggaacagg cccgtggact gctgcatcgc caggatgcat cgctggtgag cattcgttcg
    15241 tggccagaat ttatcggtac gaccagcggt tacagctata ttaaaccaaa agg
//
LOCUS       DAJVQG010000096        15236 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJVQG010000096 DAJVQG010000000
VERSION     DAJVQG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15236
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.096"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            1..749
                     /locus_tag="OQM83_002693"
                     /pseudo
     CDS             1..749
                     /locus_tag="OQM83_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF945 family protein"
     gene            complement(790..1722)
                     /locus_tag="OQM83_002694"
     CDS             complement(790..1722)
                     /locus_tag="OQM83_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCS1520918.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            2086..4872
                     /gene="polA"
                     /locus_tag="OQM83_002695"
     CDS             2086..4872
                     /gene="polA"
                     /locus_tag="OQM83_002695"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCS1520919.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(5254..5886)
                     /locus_tag="OQM83_002696"
     CDS             complement(5254..5886)
                     /locus_tag="OQM83_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCS1520920.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6468..6977
                     /gene="yihI"
                     /locus_tag="OQM83_002697"
     CDS             6468..6977
                     /gene="yihI"
                     /locus_tag="OQM83_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCS1520921.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            7166..8539
                     /gene="hemN"
                     /locus_tag="OQM83_002698"
     CDS             7166..8539
                     /gene="hemN"
                     /locus_tag="OQM83_002698"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCS1520922.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8729..8839)
                     /locus_tag="OQM83_002699"
     CDS             complement(8729..8839)
                     /locus_tag="OQM83_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCS1520923.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8951..10360)
                     /gene="glnG"
                     /locus_tag="OQM83_002700"
     CDS             complement(8951..10360)
                     /gene="glnG"
                     /locus_tag="OQM83_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCS1520924.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10372..11421)
                     /gene="glnL"
                     /locus_tag="OQM83_002701"
     CDS             complement(10372..11421)
                     /gene="glnL"
                     /locus_tag="OQM83_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCS1520925.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11595..13004)
                     /gene="glnA"
                     /locus_tag="OQM83_002702"
     CDS             complement(11595..13004)
                     /gene="glnA"
                     /locus_tag="OQM83_002702"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCS1520926.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13377..15200
                     /gene="typA"
                     /locus_tag="OQM83_002703"
     CDS             13377..15200
                     /gene="typA"
                     /locus_tag="OQM83_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCS1520927.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
BASE COUNT         3965 a         3801 c         3915 g         3555 t
ORIGIN      
        1 agtcgaaatt taatcagatt aacattatca atcacgggga agacgttgcc caaatcgatg
       61 ctttcgttgc aaaaaccatg ctggatcgcg ttaaagacaa agattatatc aatgtcaatc
      121 tgacctacga acttgataag ttaacaaaag ggaatcagca actcggtagt ggtgagtggt
      181 cattgattgc tgaatctatt gatccctcag cagtgcgcca atttatcatc cagtataaca
      241 ttgctatgca gaagcagctt gctgtacacc ctgagttagc aaacgatgaa gttgctctgc
      301 aagaagtgaa tgctgcattg ttcaaagagt atttaccgtt attacaaaaa agtgagccga
      361 ccattaaaca accggtaaga tggaagaacg cactcggcga actaaatgcc aatctggata
      421 tcagtattgc cgacccagcc aaatcttcat catccacaaa caaagatatc aaattgctca
      481 attttgatgt gaagttaccg cttaatgtcg tcacagaaac cgcaaaacag cttaatttat
      541 ctgaaggaat ggatgcggaa aaagcgcaaa agcaggctga taaataaatc agcgggatga
      601 tgacattggg tcagatgttt cagttaatca cgattgacaa caataccgcc tcgctgcagc
      661 tgcgttatac accgggtaaa gttgtgttta acggacagga gatgagcgaa gaagaattta
      721 tgtctcgtgc cggacgtttt gttcattaaa atactcactc gcctctgctc attcagaggc
      781 gagtaaaact tacttattct gacgctgtga tgacatgaga gacgtcagaa gccggtcttt
      841 ttcctgccac aatgagttca gccattgctg aaaatggcgc ttaaagcttt tgtcattgat
      901 gtagtcgcca tgtaactcat cggcaatagg ctgtaaatcc acatgtacga caatccgcgt
      961 cagtttaccg cttaacatat cgaagaacgg ctgacggtta ttgtccggat aacacagcgt
     1021 aacattaagc agtttatcga attgtttacc cagtacattt agcgccatcg caatgcctgc
     1081 cgcctttggt ggcaacaagt tttgaaaagt ggagtgggtt tgctgatgtt tttcttgcgt
     1141 gaaacgggag ccttcaacga aattgacaat ggtggtggga tgcaggcgaa acttttcaca
     1201 agaacgccga gtggtttcaa catctttacc acgtcgctcc ggatgacgta ataaataagc
     1261 gcgggaataa cgcttcataa acggcatatc cagcgcccag cacgccaggc caagaaatgg
     1321 cacccaggcc agctgttgct tgaggaaata tttattcatc ggaatgtgct tacgaaacag
     1381 cacgcacagt acgacaatat ctgcccaact acggtggtta caaataagca gataccagtt
     1441 cttcttactt aacccttcca gcccgtgaac ttcccattgc aggtgtgggt tcagatgcag
     1501 taataccgcc agaccttcac accagcaata catcataaaa tcacaaaaac gcgaaacctt
     1561 tcgccaaatg actggcacag gtagcaatag ttttacaatc ccggcaatga tgatcgggac
     1621 ggaacaaaaa atagtgacca aaatggtcaa tacgatactc agcaaaaggg ttatcgcagc
     1681 gagtattctc gtcataatga atttattcaa aaggttagcc atagacagtg cgctaaagaa
     1741 gcgcaaggcg cagattttag cagaaaacga cccaaataac ggatgatcct taaggagaaa
     1801 aataatccat atctatccac attagaaaaa atcccattat ctcaattatt agggatggct
     1861 ttatttttaa ctgcatgaaa aacaaagaca aacatcatgc tgtaaaaagc atgataataa
     1921 attaaaagcg atgtaaataa tttatgcaca aagttatcca catgacgatt tgcgagcgat
     1981 ccagaagatc tacaaaagat tttcacgaaa agcggtgaaa aactcatgtt ttcatcctgt
     2041 ctgtggcatc ctttacccat aatctgataa acaggcacgg acattatggt tcagatcccc
     2101 caaaatccac ttatccttgt agatggttca tcttatcttt atcgcgcata tcacgcgttt
     2161 cccccgctga ctaacagcgc aggcgagccg accggtgcga tgtatggtgt cctcaacatg
     2221 ctgcgcagtc tgatcatgca atataaaccg acgcatgcag cggtggtctt tgacgccaag
     2281 ggaaaaacct ttcgtgatga actgtttgaa cattacaaat cacatcgccc gccaatgccg
     2341 gacgatctgc gagcacaaat cgaacccctg cacgcgatgg ttaaagcgat gggactgccg
     2401 ctgctggcgg tttctggcgt agaagcggac gacgttatcg gtactctggc gcgcgaagcc
     2461 gaaaaagccg ggcgtccggt gctgatcagc actggcgata aagatatggc gcagctggtg
     2521 acgccaaata ttacgcttat caacaccatg acgaatacca tcctcggacc ggaagaggtg
     2581 gtgaataagt acggcgtgcc gccagaactg atcatcgatt tcctggcgct gatgggtgac
     2641 tcctccgata acattcctgg cgtaccgggc gtcggtgaaa aaaccgcgca ggcattgctg
     2701 caaggtcttg gcgggctgga tacgctgtat gccgagccag aaaaaattgc tgggttgagc
     2761 ttccgtggcg cgaaaacaat ggcagcgaag ctcgagcaaa acaaagaagt tgcttatctc
     2821 tcataccagc tggcgacgat taaaaccgac gttgaactgg agctgacctg tgaacaactg
     2881 gaagtgcagc aaccggcagc ggaagagttg ttggggctgt tcaaaaagta tgagttcaaa
     2941 cgctggactg ctgatgtcga agcgggcaaa tggttacagg ccaaaggggc aaaaccagcc
     3001 gcgaagccgc aggaaaccag tgttgcagac gaagcgccag aagtgacggc aacggtgatt
     3061 tcttatgaca actacgtcac catccttgat gaagaaacac tgaaagcgtg gattgcgaag
     3121 ctggaaaaag cgccggtatt tgcatttgat accgaaaccg acagccttga taacatctct
     3181 gctaacctgg tcgggctttc ttttgctatc gagccaggcg tagcggcata tattccggtt
     3241 gctcatgatt atcttgatgc gcccgatcaa atctctcgcg agcgtgcact cgagttgcta
     3301 aaaccgctgc tggaagatga aaaggcgctg aaggtcgggc aaaacctgaa atacgatcgc
     3361 ggtattctgg cgaattacgg cattgagctg cgtgggattg cgtttgatac catgctggag
     3421 tcctacattc tcaatagcgt tgccgggcgt cacgatatgg acagcctcgc ggaacgttgg
     3481 ttgaagcaca aaactatcac ttttgaagag attgcgggta aaggcaaaaa tcaactgacc
     3541 tttaaccaga ttgccctcga agaggccgga cgttacgccg ccgaagatgc ggatgtcacc
     3601 ttgcagttgc atctgaaaat gtggccagat ctgcaaaaac acaaagggcc gttgaacgtc
     3661 ttcgagaata tcgaaatgcc gctggtgccg gtgctttcac gcattgaacg taacggtgtg
     3721 aagatcgatc cgaaagtgct gcacaatcat tctgaagagc tcacccttcg tctggctgag
     3781 ctggaaaaga aagcgcatga aattgcaggt gaggaattta acctttcttc caccaagcag
     3841 ttacaaacca ttctgtttga aaaacagggc attaaaccgc tgaagaaaac gccgggtggc
     3901 gcgccgtcaa cgtcggaaga ggtactggaa gaactggcgc tggactatcc gttgccaaaa
     3961 gtgattctgg agtatcgtgg tctggcgaag ctgaaatcga cctacaccga caagctgccg
     4021 ctgatgatca acccgaaaac cgggcgtgtg catacctctt atcaccaggc agtaactgca
     4081 acgggacgtt tatcgtcaac cgatcctaac ctgcaaaaca ttccggtgcg taacgaagaa
     4141 ggtcgtcgta tccgccaggc gtttattgcg ccagaggatt atgtgattgt ctcagcggac
     4201 tactcgcaga ttgaactgcg cattatggcg catctttcgc gtgacaaagg cttgctgacc
     4261 gcattcgcgg aaggaaaaga tatccaccgg gcaacggcgg cagaagtgtt tggtttgcca
     4321 ctggaaaccg tcaccagcga gcaacgccgt agcgcgaaag cgatcaactt tggtctgatt
     4381 tatggcatga gtgctttcgg tctggcgcgg caattgaaca ttccacgtaa agaagcgcag
     4441 aagtacatgg acctttattt cgaacgctac cctggcgtgc tggagtatat ggaacgcacc
     4501 cgtgctcagg cgaaagagca gggctacgtt gaaacgctgg acggacgccg tctgtatctg
     4561 ccggatatca aatccagcaa tggtgctcgt cgtgcagcgg ctgaacgtgc agccattaac
     4621 gcgccaatgc agggaaccgc cgccgacatt atcaaacggg cgatgattgc cgttgatgcg
     4681 tggttacagg ctgagcaacc gcgtgtacgt atgatcatgc aggtacacga tgaactggta
     4741 tttgaagttc ataaagatga tgttgatgcc gtcgcgaagc agattcatca actgatggaa
     4801 aactgtaccc gtctggatgt gccgttgctg gtggaagtgg ggagtggcga aaactgggat
     4861 caggcgcact aagattcgcc tgaacatgcc cttttttcgt aagtaagcaa cataagctgt
     4921 cacgttttgt gatggctatt agaaattcct atgcaacaac tgaaaaaaaa ttacaaaaag
     4981 tgctttctga actgaacaaa aaagagtaaa gttagtcgcg tagggtacag aggtaagatg
     5041 ttctatcttt cagacctttt acttcacgta atcggatttg gctgaatatt ttagccgccc
     5101 cagtcagtaa tgactggggc gttttttatt gggcgaaaga aaagatccgt aatgcctgat
     5161 gcgctatgtt tatcaggcca acggtagaat tgtaatctat tgaatttacg ggccggatac
     5221 gccacatccg gcacaagcat taaggcaaga aaattattcg ccgtcctgcg tttcttctac
     5281 aggctgcatc tcgctaaacc aggtatccag tttctgccgc agcttgtcca cgccttgttt
     5341 cttcaacgaa gaaaacgttt caacctgcac atcaccgtta aacgccagta cagcttcacg
     5401 caccatattc aattgcgctt tacgtgcgcc gcttgccagt ttgtccgctt tggtcagcag
     5461 caccagaacg gcgatattgc tgtctaccgc ccactcaatc atctgctgat ccaaatcttt
     5521 cagcggatgg cgaatatcca ttagcaccac cagaccttgc aggctctgac gtttttcgag
     5581 gtattcgccg agcgcacgct gccatttgcg cttcatctct tccgggactt ccgcataacc
     5641 gtacccaggc aagtcaacca gacgcttgcc gtcagccact tcaaacaggt tgataagctg
     5701 ggtgcgccct ggggtttttg aggtacgagc caggcttttc tggttagtca gcgtgttcag
     5761 cgcgctggat ttacctgcgt tggaacggcc tgcaaaagcc acttcaattc cggtatcgga
     5821 aggtaggtgg cgaatatcag gcgcactcat cacaaaatgc gtctgttgat aattcaaatt
     5881 agtcaaagcg gtcgtctccg tcagtcaaag ctttgtggcg attatacctg aaccataata
     5941 aaaggcggat ttttcggcgt gagcgttgta agtaaaagcc atacgctttg tgagacattg
     6001 ccgatagtct ttatgcgaaa tagcagagaa aattctgcga tgcatgtcaa ataagctata
     6061 taaatcagtg aattgctttt atataaaact ctgaaaatgt gcaaatactg atggcggttg
     6121 attgtttgtt taaagcaaag gcgtaaagta gcacccatag agcgaggacg ctaacaggaa
     6181 caatgactca ggatgagggt caggagcgcc aggaggcgaa gacagaggat tgtcaggaag
     6241 acaaacgtcc ggagacgtaa ttaaacggaa atggaatcaa cacggattgt tccggctaaa
     6301 ggaaaaacag ggtgtgttgg cggcctgcaa ggattgtaag acccgttaag ggttatgagt
     6361 caggaaaaaa ggcgacagag taatctgtcg ccttttttct ttgcttgctt tctgttagat
     6421 tccgccgcaa atctatactg aataaaacgg ctaaaagacg aaccattatg aaaccatcat
     6481 cttcaaactc acgcagcaaa ggtcatgcaa aagcgcgtcg aaaaacacgc gaggagctgg
     6541 atcaggaagc tcgtgatcgc aagcgtcaga aaaaacgtcg tggtcatgcg ccgggcagcc
     6601 gcgcagcggg cggaaacacc acgtcaggca gcaaaggcca gaacgcacca aaagatccac
     6661 gtattggcag taaaacccct attccattgg gcgtggctga aaaagtcacc aaacagcaca
     6721 aaccgaagag tgagaaacct atgctttcac cgcaggcgga gttggaatta ctggaaacgg
     6781 atgagcgtct ggatgcgctg ctggaacgtc tggaagcagg cgaaaccctg agcgccgaag
     6841 agcaatcctg ggtggatgct aaactggatc gtattgatga gttgatgcag aaactcggcc
     6901 tctcttatga cgatgacgaa gaagaggaag aagacgagaa gcaagaagac atgatgcgtc
     6961 tgctgcgggg taactaacgg attgccaccg tgggccttcc cgttctgctg ataacccttc
     7021 cggttatatg ttatctggtg tggttattcg ttaaactaca gcggttgtcg cggcgacaaa
     7081 agtggctgcg caaccggctg atgacccgaa acgggcatcc gccggtacgc cgtagccgcc
     7141 agagacgcca tcggaaggag tgagcatgtc tgtacagcaa atcgactggg atctggccct
     7201 gatccagaaa tataactatt ccgggccacg atacacctcg tacccgaccg cgctggagtt
     7261 ttcagaagac ttcggcgaac aggcgttttt acaagccgtg gcgcgctatc ctgagcgtcc
     7321 attatctctc tacgtacata tcccgttctg ccataagctt tgttacttct gcggttgcaa
     7381 taagattgtt actcgccagc agcacaaggc cgatcagtat ctggacgcgc tggagcaaga
     7441 aatcgtccat cgtgcaccgc tgttcgccgg gcgtcacgtc agccaattgc actggggcgg
     7501 cggaacgccg acgtatctga ataaagcgca aatcagccgc ctgatgaagc tgctgcgcga
     7561 aaacttccag ttcaatgccg atgcggagat ttcgatcgaa gtcgatccgc gggaaatcga
     7621 actggatgta ctcgatcatt tacgcgccga aggctttaat cgcctgagca tgggcgtgca
     7681 ggacttcaac aaagaagtgc aacgtctggt taaccgcgag caggatgaag agttcatctt
     7741 tgcactgctt aaccatgcgc gtgagattgg ttttacctcc accaacatcg acctgattta
     7801 cggcctgccg aaacagacgc cggagagttt cgcctttacc ctgaaacgtg tggcggagct
     7861 gaaccccgat cgtctgagtg tctttaacta cgcgcatctg ccgaccattt ttgctgctca
     7921 gcgcaaaatc aaagatgctg acctgccgag tccgcagcaa aaactcgata tcctgcagga
     7981 aaccatcgcc ttcctgacgc aatcgggcta tcagtttatc ggtatggatc actttgcccg
     8041 tccggatgac gagctggcgg tggcccagcg tgaaggcgtg ctgcatcgta acttccaggg
     8101 ctacaccact cagggcgata ccgatctgct ggggatgggc gtttccgcca tcagcatgat
     8161 tggcgactgc tacgcgcaga accagaaaga gttgaagcag tactatcagc aagtggatga
     8221 acaaggcaat gctctgtggc gtggtattgc gctaacgcgt gatgactgta ttcgccgcga
     8281 tgtgattaag tcgctcatct gcaacttccg tctggattac gcccctattg agaaacagtg
     8341 ggatttgcac ttcgctgatt actttgcgga agatctcaag ctgctcgccc cgttagcaaa
     8401 agatgggctg gtggatgtgg atgagaaggg aatacaggtg acggcgaaag gtcgcttgct
     8461 gatccgcaac atttgcatgt gctttgatac ctatctgcgc cagaaagcgc ggatgcagca
     8521 gttctcacgg gtgatttaaa tagtgaatgg cgcttcgttt acaaagtaga aaacgaaagc
     8581 gccatcaatg tgctgagaac aagattgcct gatgcgctac gcttatcagg cctacaaaac
     8641 ctgttgaaaa tattgctttt gtaggccgga taaggcgttc acgccgcatc cggcatgaac
     8701 aaagagcact tcatcagcaa tctgaaactc aactaaacag cccaatcatt gcggcacaca
     8761 gcacggcagc aacggcagtt tgcggtgtgt ggctgtcaac tgcgccactc gataccagat
     8821 taattattga ttccagcata atgactctcc ccgttttccg ggcaagatca tactgaactt
     8881 atcggaacag taaagcgtaa aataccagca atttgcgctc aataatcaat ctttacacac
     8941 aagctgtgaa tcactccatc cccagctctt ttaacttacg cgtcagggtg ttgcggcccc
     9001 agccgagtag ccgcgccgct tcctgtttat gcccctgcgt atgtcgtaac gcggtcgtca
     9061 gtaacgtccg ctccagctct ggctgcgctt cggaaagcag attttgatga ccggaacgca
     9121 gcgctctgtc tgcccactgc gcaagaagcg ttgcccagct gtccggttgc atttgcgaag
     9181 tactctccgc aaccgttgat tcaaacagtt cgccgggcaa atcctgaatc aacacttcct
     9241 gcccggcggc catcaccgtt agccagcggc aggtgttttc cagctggcgc acgttgcctg
     9301 gccacgccag acgcgtcaga gcagcttcgg tttccggatg cagtaacttc gcttctacgc
     9361 ccagttcgcg cgcggcaacc tgtaaaaaat ggcgcgccag acggggaata tcttcccgac
     9421 gttcgcgcag cggcggcaga tgaacgcgga taacgttcag gcggtggaac agatcctcac
     9481 ggaacttacc ttcctgcact cgctgttcga gattctggtg agtggcagcg ataatacgca
     9541 catccacttt caccggcgca tagccgccaa cgcggtaaaa ctgaccgtct gccagcacgc
     9601 gcagcaaacg cgtctgcaca tccagcggca tatcaccaat ttcgtcgagg aataatgtac
     9661 cgccatcggc ctgttcaaaa cgcccctgac gaatggtatt cgcgccagta aacgcgcctt
     9721 tctcgtggcc aaacagttct gattcgatca aatcttttgg gatagctgcc atattcagcg
     9781 cgataaacgg cgctttggcg cgcggactgt ggcgatgcag ggcatgagcg accagttctt
     9841 taccggtgcc ggattcgccg ttaatcagca cgctaataga agaacgcgaa agccgaccga
     9901 taatacggaa cacgtcctgc atggctggcg cttcgccgat gatatcggtc gttgggccgt
     9961 taagctgaat attacgcggc tgctgctgtt cctggtaatg actgatagcg cgctcaacca
    10021 gcgccactgc ttcgtcgata tcaaacggtt tgggcagata atcaaacgcc ccttgttgat
    10081 aggcgctgac ggcagcatcc agatcggaat gtgcggtcat aatgatgacc ggaagcattg
    10141 gatggcgctg tttaatctgc ttgagcagcg ccagcccgtc cattcccggc atacggatat
    10201 ctgaaagcag cacatccggc gttttgctcg ccagcgcctc cagtacttcc gcgccgttct
    10261 caaatgtcgt acaggttaaa cccgctccag cgagcgcacg ttcaagcacc caacggatgg
    10321 aactatcgtc atcgactacc cagactatcc ctcgttgcat aaacgtcacc tttatttcct
    10381 gataggcagg taaaccgaga actcggtatg acctggccaa ctggtaaatt caattttgcc
    10441 tgaatgctga tcaatcaaat tacgggcgat ggataagcca agcccggtgc caccttcgcg
    10501 gccgctgacc atcgggtaaa acagcgtatc ctgcaaatga ggcggaatgc ccggcccgtt
    10561 atcttccaca tcaatccgcg ccgccagccg gtagcgctcg ccgtgtaagg tcagttgaaa
    10621 cgcggtgcgg gtacgcagaa tgatttcacc gccttccggc cccagcgcct gtagcgcatt
    10681 gcgcacaata ttcagcaaga cctgttcaat ttgatccggg tcgtgcgcca gttccggcag
    10741 gctggggtcg taatcacgaa tcaaccgcac gttgtccggc agttccatcg acaccagcgt
    10801 caccacgcgt tcagctactt tgtgaatact ttcggtaacg cgcgtaccgg gcagctgcgg
    10861 ccccaacaga cggtcgacca gatttcgcag ccggtccgcc tgttcgataa tcactttggt
    10921 atattcgagt agtgatgggt caggtaacgc tttgctgagc agctgcgccg cgccacgtaa
    10981 acctccaagc ggatttttaa tctcatgtgc caggccgcgc actaaatcac gggcagcaac
    11041 ctgctgggcg tgctgtagct gttcctgact taagcggcgc tggttatcca tcggagccat
    11101 ctccagcagg atcatgccgt ccggcatacg ctgggccgtc acagaaagga tatgcgagcg
    11161 cccgtcgatg accagcgtca cttcgttatc ggtaaaacct tgccccgcct ccagactttc
    11221 ttgcatcagc tcgatattta atgagaagta gctcaacagt tccggtaacg gtgtaccaaa
    11281 caatttgcgg gagctttggg cgagcagttg ttgcgcggcg gggttggcgt aatggatcgc
    11341 cagattgtca tcaattaaca aaatactgtt aatcagcgag ttgaggatct gcccagcatc
    11401 gggctgcgtg cctgttgcca taaagcagtc tcctgaacag gttgcaccat tttagtgcat
    11461 tatagctttt tacggataaa aagcgcgaag catcagagaa ttgacggaga aaaaagccca
    11521 tgcagagatg ggctgaaagt ttccacggca actaaatccc ggcgttgttg cgccgggtag
    11581 tacaacttca actattagac gctgtagtac agctcaaact ctaccggatg cggagtcata
    11641 cgcacgcggt catcttcttc gcgacgtaga gcgatgtacg catcaattgc ttcgtcagtg
    11701 aacacgccac cggctttcag gaactcgcgg tccagatcca gttcgttcag tgcttcttcc
    11761 agagagcctg caacctgtgg gatctctttc gcttcttctg gcggcaggtc atacaggttt
    11821 ttgtccatgg cttcgcccgg atggatcttg ttcttgatac catcaagacc ggccatcagc
    11881 agggcagcaa agcacaggta cgggttagct gccggatccg ggaaacgtac ttcgatacga
    11941 cgtgctttcg gagaagaaac caccggaata cggatagacg cagaacggtt acgcgcagag
    12001 taagccagca ttaccggtgc ttcatagccc gggaccagac gcttataaga gttggtggtc
    12061 gggtttgcca gggcgttaat cgctttagcg tgtttgatta cgccgccaat gtagtacagc
    12121 gcctgctcag acagacctgc gtatttgtcg cctgcgaaca ggttaacgcc gtttttagac
    12181 agagacatgt ggcagtgcat accggagccg ttatcaccga acatcggttt tggcataaag
    12241 gtcgcggttt taccgaagcg gtgcgctacg ttgtgcacaa catatttgta gatctgaatt
    12301 tcgtcagctt ttttggtcat agtattgaag cgggtagcca cttcgttctg accagcagtc
    12361 gctacttcgt ggtgatgggc ttcaaccacc agacccatct gttccatcac cagacacatt
    12421 tcagaacgaa tatcctgagc cgagtctacc ggtggaaccg ggaagtaacc gcctttcact
    12481 gccggacggt gacctttgtt accaccttcg tattgggtgg aggagttcca tgcgccttcg
    12541 atatcgtcga tagcaacgtg ggaaccggag atagatgatc cgaaacggat gtcatcgaac
    12601 aggaagaatt caggttctgg cccgaacagt acggtgtcgg caatgccagt ggaacgcagg
    12661 taatcttcgg cgcgcttcgc aatggagcgc gggtcacggt catagccttg cagggtgcca
    12721 ggttcaagga tgtcgcaacg gataatcagg gtggagtcgg cgaagaacgg gtcaatcact
    12781 gcggtggatg cgtctggcat cagcaccatg tcggactcgt taatgccttt ccagccgcca
    12841 atcgaggagc cgtcaaacat tttgccttct tcgaagaatt cagcattcac ctgatgagca
    12901 gggatagtga cgtgctgttc tttaccttta gtatcggtga agcgcaaatc aacaaacttc
    12961 acttcgtgct cgttcagcat cgtcagtacg tgttcagcgg acatacttta actctcctgg
    13021 attggtcatg gtcgtcgtgg taacgaaatc tgcaattatt ttggccgtgt cgccgtaaaa
    13081 aagataaagc gaaatctgtg ccaactttta aattgcccct aaaaggcgtt atcatgcgca
    13141 ccatcgtgca aaagggctgc accacgatgt gaatgttgca ccaatatagt gcttcaatgg
    13201 aaacattaag caccatgttg gtgcaatgac ctttggataa ccctttttat gctccgtgaa
    13261 agcgatcaca aagggactct gcaatacttg tttgcggagg atgtttgtga tcctgttttg
    13321 tagtgcgatt aatccgtgta caataacgcg ctatttctaa tgcctgaggc aaagttgtga
    13381 tcgaaaaatt gcgtaatatc gccatcatcg cgcacgtaga ccatggtaaa accaccctgg
    13441 tagacaagct gctccaacaa tccggtacgt tcgactctcg tgccgaaacc caagagcgcg
    13501 tgatggactc caacgatttg gagaaagagc gtgggattac catcctcgcg aaaaacaccg
    13561 ctatcaaatg gaatgattac cgtatcaaca tcgttgatac cccggggcac gccgacttcg
    13621 gtggtgaagt tgaacgtgta atgtccatgg tagactcagt gctgctggtg gttgacgcat
    13681 ttgacggccc gatgccgcaa acgcgcttcg taaccaaaaa agcgtttgct tacggcctga
    13741 agccgattgt tgttatcaac aaagttgacc gccctggcgc gcgtcctgat tgggttgtgg
    13801 atcaggtatt cgatctgttc gttaacctcg acgcgaccga cgagcagctg gacttcccga
    13861 tcgtttacgc ttctgcgctg aacggtatcg cgggtctgga ccacgaagat atggcggaag
    13921 acatgacccc gctgtaccag gcgattgttg accacgttcc tgcgccggac gttgaccttg
    13981 acggtccgtt ccagatgcag atttctcagc tcgattacaa cagctatgtt ggcgttatcg
    14041 gcattggccg catcaagcgc ggtaaagtga agccgaacca gcaggtcact atcatcgata
    14101 gcgaaggcaa aacccgcaac gcgaaagtcg gtaaagtgct gggccacctc ggtctggaac
    14161 gtatcgaaac cgatctggcg gaagctggcg atatcgtggc gatcacgggc cttggcgaac
    14221 tgaacatttc tgacaccgtt tgcgacacgc aaaacgttga agcgctgccg gcactctccg
    14281 ttgatgagcc gaccgtttct atgttcttct gcgttaacac ctcgccgttc tgcggtaaag
    14341 aaggtaagtt cgtaacgtct cgtcagatcc tggatcgtct gaacaaagaa ctggtacaca
    14401 acgttgcgct gcgcgtagaa gaaaccgaag acgccgatgc gttccgcgtt tctggtcgtg
    14461 gcgaactgca cctgtctgtt ctgatcgaaa acatgcgtcg tgaaggtttc gaactggcgg
    14521 tatcccgtcc aaaagtaatc ttccgtgaaa tcgacggtcg taaacaagag ccgtatgaaa
    14581 acgtgactct ggacgttgaa gaacagcatc agggttctgt aatgcaggcg ctgggcgaac
    14641 gtaaaggcga cctgaaaaac atgaatccag acggtaaagg ccgcgtacgt ctcgactacg
    14701 tgatcccaag ccgtggtctg attggcttcc gttctgagtt catgaccatg acttccggta
    14761 ctggtctgct gtactccacc ttcagccact acgacgacgt acgtccgggt gaagtgggtc
    14821 agcgtcagaa cggcgtactg atctctaacg gtcagggtaa agcggtcgcg ttcgcactgt
    14881 tcggtctgca ggaccgcggt aagctgttcc tcggtcacgg tgcagaagtt tacgaaggtc
    14941 agattatcgg tattcacagc cgctctaacg acctgactgt aaactgcctg accggtaaga
    15001 aactgaccaa catgcgtgct tccggtactg acgaagctgt tgttctggtt ccgcctatcc
    15061 gcatgactct ggaacaagct ctggagttca tcgatgatga cgaactggta gaagtgactc
    15121 cgacctctat ccgtattcgt aaacgtcacc tgacggaaaa cgatcgtcgt cgcgccaacc
    15181 gcgcaccgaa agacgattaa tttcgttttt tagttataaa aaacctgcca tcgctg
//
LOCUS       DAJVQG010000097        15223 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJVQG010000097 DAJVQG010000000
VERSION     DAJVQG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15223
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.097"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            1..931
                     /locus_tag="OQM83_002704"
                     /pseudo
     CDS             1..931
                     /locus_tag="OQM83_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            complement(1019..1990)
                     /gene="lpxM"
                     /locus_tag="OQM83_002705"
                     /gene_synonym="msbB"
     CDS             complement(1019..1990)
                     /gene="lpxM"
                     /locus_tag="OQM83_002705"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCS1520928.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(2110..3432)
                     /gene="mepM"
                     /locus_tag="OQM83_002706"
     CDS             complement(2110..3432)
                     /gene="mepM"
                     /locus_tag="OQM83_002706"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCS1520929.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(3448..4380)
                     /gene="znuA"
                     /locus_tag="OQM83_002707"
     CDS             complement(3448..4380)
                     /gene="znuA"
                     /locus_tag="OQM83_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCS1520930.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4459..5214
                     /gene="znuC"
                     /locus_tag="OQM83_002708"
     CDS             4459..5214
                     /gene="znuC"
                     /locus_tag="OQM83_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCS1520931.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            5211..5996
                     /gene="znuB"
                     /locus_tag="OQM83_002709"
     CDS             5211..5996
                     /gene="znuB"
                     /locus_tag="OQM83_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCS1520932.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(6143..7153)
                     /gene="ruvB"
                     /locus_tag="OQM83_002710"
     CDS             complement(6143..7153)
                     /gene="ruvB"
                     /locus_tag="OQM83_002710"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCS1520933.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(7162..7773)
                     /gene="ruvA"
                     /locus_tag="OQM83_002711"
     CDS             complement(7162..7773)
                     /gene="ruvA"
                     /locus_tag="OQM83_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCS1520934.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            complement(7912..7977)
                     /gene="yobI"
                     /locus_tag="OQM83_002712"
     CDS             complement(7912..7977)
                     /gene="yobI"
                     /locus_tag="OQM83_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCS1520935.1"
                     /translation="MYIFITHLFTEYVILKYLLPI"
     gene            8050..8652
                     /locus_tag="OQM83_002713"
     CDS             8050..8652
                     /locus_tag="OQM83_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCS1520936.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(8654..9175)
                     /gene="ruvC"
                     /locus_tag="OQM83_002714"
     CDS             complement(8654..9175)
                     /gene="ruvC"
                     /locus_tag="OQM83_002714"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCS1520937.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(9210..9950)
                     /locus_tag="OQM83_002715"
     CDS             complement(9210..9950)
                     /locus_tag="OQM83_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1520938.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(9979..10431)
                     /gene="nudB"
                     /locus_tag="OQM83_002716"
     CDS             complement(9979..10431)
                     /gene="nudB"
                     /locus_tag="OQM83_002716"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCS1520939.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(10549..12321)
                     /gene="aspS"
                     /locus_tag="OQM83_002717"
     CDS             complement(10549..12321)
                     /gene="aspS"
                     /locus_tag="OQM83_002717"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCS1520940.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            12631..13197
                     /locus_tag="OQM83_002718"
     CDS             12631..13197
                     /locus_tag="OQM83_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1520941.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            13552..13800
                     /locus_tag="OQM83_002719"
     CDS             13552..13800
                     /locus_tag="OQM83_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCS1520942.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            14401..>15223
                     /locus_tag="OQM83_002720"
     CDS             14401..>15223
                     /locus_tag="OQM83_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1520943.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPEIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
BASE COUNT         3810 a         3909 c         3524 g         3980 t
ORIGIN      
        1 ttttttcgcg gcctgggatt ttgcttcaaa ttatcgtgat ggggaatcat atgttttact
       61 cgctgaaaaa atgacaactg tgatcagcgg gcctaaaact acgatgacgc tgatttatca
      121 ctacaattcc tcttatcttc gtttagcctt taccctgaca caggatagtc cacgcgttga
      181 tgtccaaacg tttattgatt ggcacgaacc taatgtttct ctcaaagtaa aattcccggt
      241 gtcagtacaa acatctcttg cacaatgtca aattcagttc ggtgtaattg acagaccaac
      301 gcatagcgat gattcgttcg cttttgccaa agatgaaata ccagcacatc actgggtcga
      361 cctttctgat cgagaaactg gcatagcttt aatagcaaaa aacaaatatg gctatcgtgt
      421 caaagaccag acgcttgaat taactttgct gcgttcgcag cataaacctg gggaagttgt
      481 taagacagac aattatgata atttcctgat taataatttt gcagatattg gtaaacaaaa
      541 attcatattc tccttatttc ctcatcatgg agattaccgt gtgggtggtg ttattaatca
      601 agcctatata atgaatcatc ccttacaggt cgtccctgtc aaaccgcacc cgggtgaatt
      661 acccccttca ctatcatttt ttgctatcga tactaacagc gttatcatcg aaacaataaa
      721 attagctgaa gacggcgacg gtataatcgt aagattatat gagagtcatg gattagaaat
      781 ttcagccatg ctttcgtgag tatgtaatta tcactacgtt caatatgtcg atcttcaaga
      841 aaataagtta gatgacagtt tttattgcaa tcaatattgc aatcttgagt tcaaaccttt
      901 tgaaattatc acgctacgat taactcaata gcgtactaac ctaaaaactc aaaatagtgg
      961 gaacactcat tgagattttt tatcgagtac agcctctcaa taatttgaga ggcttttttt
     1021 atttgatagg ataaagatct ttacgcttat acggttggat ttcgcccggt ttgcgagttt
     1081 tcagcaattt taatatccag gtgtattgtt ctggtcgcgg accaacaaaa atctcgactt
     1141 cttcattcat ccgccgcgca atcgtatgat catccgcctc taacagatca tccatcggtg
     1201 ggcgcacctg aatcgtcaga cggtgcgtct tgccatcata aatcggaaac agcggaacaa
     1261 cgcgcgcacg gcacactttc atcaaacgac caatcgcggg caacgtcgct ttataggtgg
     1321 caaagaaatc cacaaattcg ctgtgctctg ggccatgatc ctgatcgggt aaataatatc
     1381 cccagtaccc ctgacgtacc gactggatga atggtttaat accgtcattt ctcgcatgca
     1441 gacgaccgcc aaagcgacga cgcaccgtgt tccagacata atcaaaaacc gggttgccct
     1501 gattatggaa catcgctgcc attttctgcc cttgcgaggc catcagcatg gcaggaatat
     1561 cgacggccca accgtgcggc accagaaaga taactttctc gttattacgc ctcatctctt
     1621 cgatgatctc cagcccttgc cagtcaacgc gcggctgaat tttctccggt ccgcgtattg
     1681 ccaactcagc catcattgcc atcgcttgcg gcgcggtggc aaacatctca tcaacaatcg
     1741 cttcgcgttc agcttcacta cgttctggaa agcagagcga cagattgatt aacgcacgac
     1801 ggcgtgagct ttttcccagt cgtccggcaa aacgtcccag ccgtgccaga atgggatcac
     1861 ggaactttgg cggcgttaaa gcgatacccg ccatcgctgc tacacccagc catgctcccc
     1921 agtagcgcgg gtggcgaaag gatttatcaa actcaggaat gtattcgcta ttattttttt
     1981 tcgtttccat gcttttccag tttcggataa ggcaaaaatc aatctggtga tagtatagcg
     2041 gcgcaacttg ccccgcacca aataaaaaaa gccgatactg actgcgtacc ggctgcgaat
     2101 ggatgttaat taatcaaacc gtagctgcgg cacaatctct ttggcctgcg ccaggaattc
     2161 gcgacgatcg gagccggtca gcccttcggt acgcggcagt tttgccgtca gcgggtttac
     2221 ggcctgctgg tttatccata cttcatagtg cagatgcggc ccggttgaac gtccggtatt
     2281 accggaaagc gcgatacggt cgccacgttt caccttctgt cccggtttca ccagaatctt
     2341 gcgcaagtgc atataacgcg tggtgtagct gcgaccatga cgaatagcca cataataacc
     2401 tgctgcgcca ctgcgtttgg caaccaccac ttcaccgtca cccactgaaa gcactggcgt
     2461 accctgcggc atggcgaaat caacacctct gtgtgctgca acgcgaccgg tcaccggatt
     2521 agtacgacgc gggttaaagt tagacgagat acggaactgt ttcgccgtcg ggaatcgcaa
     2581 gaatcctttc gccagaccag taccgttacg atcgtagaat ttgccatctt cagcgcggat
     2641 agcgtaataa tctttacctt ctgaacgcaa acgtacgccc agcagctggc tttgctcacg
     2701 tttaccatca agcatttctc gagacattaa caccgcaaat tcatcgcctt ttttcagttt
     2761 gcggaaatcc atttgccact gcatggcttt aatcactgcg ctcacttcgg cgctggttaa
     2821 accggcgttt ctggcgctgg caacaaagct ccccccgacg gtacctttca gcagattgtt
     2881 aacccactct ccttgctgca tttcgctggt cattttaaaa ccgttagcgg cagtacggtc
     2941 ataggttcgg gtttcacgac gagacacttc ccaggtgagg cgctgcagtt cgccgtccgc
     3001 ggttaatgtc caggagagtt gttgaccgat tttcaggtta cgcaattctt tgtcggccgc
     3061 agccagttgg gtgatatcac ccatatcaat accatactga ttgagaatgc tgcttagcgt
     3121 atcgccagtg gaaacaacat attcatgcac gcccgcttca ccggcgattt tgtcatccag
     3181 ttcgtcctgg ggaatggctt catcttcttg tgcagcttga tcaatcggct cactggcttc
     3241 aggtaagagc gaacgaattt cgttctgttc cagctcaatg gttttgacaa ttggcgtggc
     3301 atcgcggtga taaacatagg gccgccagac agcgacggcc agagtaagaa cggtgagcga
     3361 ccccaacata acgcggtgtg gtcgcggtaa attattaaac gccagggcga cagagcgggc
     3421 tatctgttgc acgtaatcac ttcctcatta atctcctttc aggcagctcg catactggtt
     3481 ggctaattga ctcaggaatt ctgaatagct tgttttaccc agtttgatat tcgtccccag
     3541 gggatccaac gttcccatac gaacggatgt ccctcgtgcg acgctctcaa cgaccgctgg
     3601 cctgaactgt ggctcagcaa aaacgcaggt tgctttttgc tcaaccaact gtgttcttat
     3661 ttcatgtaaa cgctgcgcgc caggttgaat ctcagggtta acggtaaaat gaccaagcgg
     3721 tgtcagtccg aactgttttt cgaaatagcc gtaagcatcg tgaaaaacga aataaccttt
     3781 ccccttgagc ggcgcgagct cgttaccaac ctgcgtttcg gttgaggcta attgtgcctc
     3841 aaaatccttc aggttggcgt caagtttggc tcgactttgc ggcataagtt ccactaattt
     3901 tccatggatt gcaaccgctg tagcccgcgc tatctctggg gaaagccaaa gatgcatgtt
     3961 gaaatcgccg tgatggtgat cttcgtcact tttttccgcg tggtcgtgat catcatcatc
     4021 gccgtgaata cttttcatca gtagcggttt cacatcttca agctgcgcaa tcgttacctg
     4081 cttcgctcct ggtaatttgc ttaccggttt ttgcataaac gcttccatct ccgggccaac
     4141 ccaaacgact aagtccgcgt tctgtaagcg ttttacatcc gatgggcgca gtgaataatc
     4201 atgttctgaa gcgccgtcag gaagtaaaac ctctgtttct gttaccccat cagcaatggc
     4261 agaagcgatg aacccaacgg gtttaagcga agcgacaacg gcggcatctg cggcctgtgt
     4321 tgcacctccc cagagagcgg cggataatgc tgcgaaaaga agcgtttttt tatgtaacat
     4381 aatgcgacca atcatcgtaa tgaatatgag aagtgtgata ttataacatt tcatgactac
     4441 tgcaagacta aaattaacat gacaagtctg gtttccttgg aaaatgtctc ggtttctttt
     4501 ggccaacgcc gcgtcctctc tgatgtgtcg ctggaactta aacctggaaa aattttgact
     4561 ttacttgggc caaatggcgc aggtaagtcg acactggtac gggtagtgct cgggctggta
     4621 acacccgatg aaggggttat caagcgcaac ggaaaactgc gcatcggcta tgtaccgcag
     4681 aagctgtatc tcgacaccac gttgccactg accgtaaacc gttttttacg cttacgccct
     4741 ggtacacata aagaagatat tttgcctgca ctgaaacgtg tccaggccgg gcacctgatt
     4801 aacgcaccga tgcaaaagct ctctggtggc gaaacgcagc gtgtactgtt agcgcgagca
     4861 ttgttaaatc gcccgcaatt attagtgctg gatgaaccca ctcaaggcgt ggatgtaaat
     4921 ggccaggtgg cgttatatga ccttattgac caactgcgtc gtgaactgga ttgtggcgtt
     4981 ttaatggttt ctcacgatct gcatctggtg atggcaaaaa ccgatgaagt gctgtgcctg
     5041 aatcaccaca tttgttgttc cggcacaccg gaagttgttt ccctgcatcc ggagtttatt
     5101 tcaatgtttg gtcctcgtgg tgctgaacaa ctgggtatct atcgccatca tcataaccat
     5161 cgtcacgatt tacagggacg aattgttttg cgtcggggaa atgatcgctc atgattgaat
     5221 tattatttcc cggttggtta gccgggatca tgctcgcctg tgccgcgggt ccgctgggtt
     5281 cgtttgtagt ctggcgtcgt atgtcttatt tcggtgatac gctggctcat gcctcattac
     5341 ttggtgtcgc gtttggtttg ttgctggacg tgaatccatt ctatgcggtg attgccgtta
     5401 cgctgctgct ggcgggcggt ctggtatggc tggagaagcg tccacagctg gcgatcgaca
     5461 cgttattagg gattatggcg cacagtgccc tgtcgctggg cctggtggtc gttagtctga
     5521 tgtctaatat tcgtgttgat ttgatggctt acctgttcgg tgatttgctg gcagtgacgc
     5581 cagaagatct catctctatt gcgattggcg tggtcatcgt ggtggctatt ttgttctggc
     5641 aatggcgcaa tttgctgtcg atgacgatta gcccggatct ggcgtttgtt gatggtgtga
     5701 aattacagcg cgtgaaattg ttgttgatgc tggtgacggc attgacgatt ggtgtagcga
     5761 tgaaatttgt cggtgcgttg attattactt cgttgctgat tattcctgca gctactgcgc
     5821 gtcgctttgc ccgtacgccg gaacagatgg ctggtgtcgc tgttttggtg gggatggtgg
     5881 cagtaactgg cggtttaacc ttttccgcgt tttacgatac gccggcgggt ccgtcggtag
     5941 tgctatgtgc tgcactgtta tttattatca gtatgatgaa aaagcaggcc agctaatctg
     6001 tcgctgaaca catttgtcgg atgcggcgcg agcgccttat ccgacctacg gttcgctatc
     6061 tctggtaggc cggataagac gcgaatagcg tcgcatcagg cacactgcca atgtcggatg
     6121 cggtgcgagc gaccaatccg acttacggca tttctggcgg cgttatgcca aagtgattcc
     6181 acgcccgcgt cgtcgccata cgcccacgcg gtgtacgctg caaaaagcct tgctgaatca
     6241 gataaggctc cagcacatcc tcaatggttt cacgttcttc gccaatggct gccgccaggt
     6301 tatccagacc taccggccca ccaaagaact tatcgattac cgccagcaac aatttgcggt
     6361 ccatataatc gaaaccttca gcatcgacat tcaacatatc cagcgcctga gcagcgatat
     6421 ctgccgagat ggtgccatcg tgcttcactt cggcgaaatc acgcactcga cgcagcagac
     6481 ggttggcaat acgtggcgta ccgcgagcgc gacgagcaac ttccagcgcg ccgtcatcac
     6541 tcatctcaag ccccataaag cgtgcgctgc gactgacgat atattgcaga tccggcacct
     6601 gataaaactc cagacgttgc acaataccaa aacggtcgcg caacggtgat gtcagcgaac
     6661 ctgcgcgcgt ggttgcacca atcagggtaa acggcggcaa atcaatttta atggagcgtg
     6721 ccgccggacc ttcaccaatc atgatatcca gttggtagtc ttccattgcc gggtacagca
     6781 cttcttcaac aactggcgat agacggtgga tctcatcaat aaacagcacg tcatgcggtt
     6841 caaggttagt gagcatcgct gccagatcgc ccgccttttc cagcaccgga ccagaagtcg
     6901 tgcgtaaatt aacgcccatt tcattggcga caatgttggc aagcgtagtt ttacccaacc
     6961 ccggaggacc aaaaatcaac agatgatcga gggcatcgcc gcgcagtttc gctgctttga
     7021 tgaaaatctc catctgtgaa cgaacctgcg gctgaccaac atactcttcc agtaatttag
     7081 ggcgaatggc gcgatctgcc ccatcttccg gcaaagtggt accggcagaa atcagacggt
     7141 ctgcttcaat catcctttac ctcataacgc ggcgcgtagg gcttcgcgaa ttaatgtttc
     7201 actgctggcg tcagtgcgag cgattttgct caccatgcgg cttgcttctt gtggtttata
     7261 gcccagcgcc accagcgcgg caaccgcttc ctgttcagca tcgtcggttg ccgggctggc
     7321 aggcgacgtg agcaccagat cggcggctgg cgtaaagaga tcgccgtgca aacctttaaa
     7381 tcggtctttc atttcgacaa ttaaacgctc ggcggttttt ttaccaatgc ccggcagttt
     7441 caccagtgct cccacttctt cacgttcaac ggcattaacg aactgctgcg ctgacattcc
     7501 ggagaggatc gccagcgcca acttcgggcc gacgccgttg gttttgatta actctttaaa
     7561 caacgtgcgc tcttgtttat tgttaaaacc gtacagcagt tgcgcgtctt cacgcaccac
     7621 aaagtgggtg aaaacgatcg cttcctgacc cgcttcaggg agttcataaa aacaggtcat
     7681 cggcatatgc acttcatagc ctacgccgcc cacttcaatt aacaccagcg ggggttgttt
     7741 ttcaatgatg atgcctctga gtctgcctat cacatgacgc tcctgcgtaa tgaatcaaag
     7801 ataatgctgt atgataaaaa aatgctggat agatatccag cgaaggatga agaaaacttg
     7861 cgaggtgtct cgatgaactg aaaaatggca cagtataatt tattctacag gttatattgg
     7921 aagcaaatat tttaatatta catattcagt gaataaatgc gtaataaaaa tatacattgc
     7981 gcctcctgaa aaaaaataaa ttttttatgc tattacgtat attcgtatct atttcaatgg
     8041 aatgacaacg tgaatattaa ttaccctgct gaatatgaaa ttggagatat cgtctttaca
     8101 tgtataggtg ctgccttatt tggtcaaata tcagctgcat caaattgctg gagtaatcac
     8161 gtcgggatca ttatcggtca taacggtgaa gacttcctgg ttgcagaaag ccgggttccc
     8221 ctttcaacca tcaccacgct atctcgtttt attaaacgtt cgtctaatca acgctatgct
     8281 ataaagcgat tagacgctgg gctgacagaa caacaaaaac aacgaattgt tgaacaggtt
     8341 ccctcccggc tacgcaaact ttaccacacc ggttttaaat acgaatcttc gcgccagttc
     8401 tgttcaaaat ttgtttttga tatttataaa gaagcgctat gcattccggt gggtgaaata
     8461 gaaacgtttg gagaactgtt aaatagcaat ccgaatgcaa aactcacttt ctggaaattc
     8521 tggttcttag gttctattcc gtgggagcgt aaaaccgtca cgccagccag tttgtggcat
     8581 catccgggtc tggtgttgat tcacgcggag ggagttgaaa cgcctcagcc tgaactgacc
     8641 gaggcggtat aacttaacgc agtcgccctc tcgccaggtt cagccgcgat tcgctcatct
     8701 gcattgcatt ctggctgacg tgacaatggg taatcgcgat agctaacgca tcggcggcat
     8761 ccgcctgtgg attagcgggc agtttcagca aggtgcggac catatgctgc acctggcttt
     8821 tttcggcact accaatacct accaccgttt gctttacctg acgtgccgcg tattcaaata
     8881 ccggcaattc ctgattcacc gccgccacaa tcgccacgcc gcgcgcctgc cccagtttca
     8941 gagccgagtc ggcatttttg gccataaaga cttgttcaat ggcgaaataa tctggctgga
     9001 actgggtgat gatttccgtc acgcccgcat agatgagctt cagacgagac ggtaaatcat
     9061 ccactttggt gcgtatgcat ccgctaccca ggtaggacag ttgcctgcct acctggcgga
     9121 tgacaccata gccgatcacg cgcgaacccg ggtcaatgcc gagaataata gccatcacgc
     9181 gtctccgttt tgctgtttag caggccccat cagagagttg ctgcaacctc atcagagatt
     9241 tcaccgttat ggtagacttc ctgcacgtcg tcgcaatctt ccagcatatc gatcagacgc
     9301 atcagtttcg gtgcggtttc tgcatccata tcagctttgg tggacgggat catggaaact
     9361 tccgcgctgt ctgctttcag acctgccgct tccagagcgt cgcgcacttt acccatttct
     9421 tcccatgcag tgtagacgtc aatcgcgccg tcatcatagg tcacaacgtc ttcagcacct
     9481 gcttccagtg ctgcttccat aatggtgtct tcatcgcctt tctcgaagga gatcacgcct
     9541 tttttgctga acagataggc aacggaacca tcagtaccga ggttaccgcc acatttgcta
     9601 aatgcatgac gcacttcagc aacggtacgg ttgcggttgt cagacagaca ttcaatcatg
     9661 attgccgtgc cgccaggacc gtaaccttcg tagatgatgg tttccatgtt tgcatcatca
     9721 tcaccgccca caccacgtgc aattgcgcgg ttcagtgtgt cacgggtcat gttgttagac
     9781 agtgctttat ccaccgccgc acgcagacgc gggttagcgt ccggatcgcc accgcccagc
     9841 ttagccgcgg ttaccagctc acgaatgatt ttagtgaaga ttttaccgcg cttagcatcc
     9901 tgcgcagctt tacgatgtct ggtgttggcc catttactat gacctgccat aaaaatatct
     9961 ccagatagcc ctgcctgttc aggcagcgtt aattacaaac tgttcaatcg cctgccggtt
    10021 gctccaggac ttagtgagcg ccgcggcagc aggcgcatca agccacttgt aagccagatg
    10081 ttcagtgaag actacctggc gttcgtgcgg gagcgcaaga cagaaccatg attccgtatt
    10141 acgcgtcacg cccggcgcat agcgatgacg taaatgtgaa aaaatttcaa actctaccgt
    10201 gcgctgacag tcaattaagg tcagttgttc agcgacaaca tcaatggtga cctcttcctt
    10261 tacttcgcgc atggcagctt gcggcgcggt ttcaccctct tccacgctgc cggttaccga
    10321 ctgccagaaa tcgggatcgt cacgccgctg caacatcagc acccgtttcg tatcttgtgc
    10381 atagatgacc actaagatcg aaacgggacg cttatacact ttatccttca cgctgcctct
    10441 tcgttgactg ccttcgctca tcccattcac atagttatct atgctcatgg gagttcactc
    10501 agttgccgcc ttgatgcaac gcgaatgatt tcgtgtattt gagtcatatc agttattctc
    10561 agccttcttc acaacctgaa tgctcagctc agccagtaca gtcgggttag caaagctcgg
    10621 tgcttcagtc atcagacacg ctgccgccgt ggttttcggg aaggcgataa cgtcacggat
    10681 attgtcggtg ccagtcagca gcatggtcag acggtcaaga ccgaatgcca gacctgcgtg
    10741 cggcggagta ccgtatttca gggcgtcgag caggaagccg aatttctcgc gctgttcctc
    10801 ttcgttgata cccagaatac caaacaccgt ctgctgcata tcaccattat ggatacgtac
    10861 tgaaccaccg cccacttcgt aaccattgat gaccatatcg taagcgttcg ccaccgcatt
    10921 ttccggtgca gctttcagtt ctgcagcagt catgtctttc ggtgaggtga acggatggtg
    10981 cattgccgtc aggccgcctt caccgtcgtc ttcaaacatc gggaagtcga taacccacag
    11041 cggtgcccat ttgctttcgt cggtcagacc aaggtcttta cccactttca ggcgcagtgc
    11101 gcccatcgcg tcggcaacaa ttttcttgtt gtcggcaccg aagaaaatca tatcgccatc
    11161 ttgcgcggca gtacgatcca ggatggcttc gatgatttct gcattaagga acttcgctac
    11221 cgggctgttg ataccttcca gacctttcgc gcgttcgtta actttgatgt aagccagacc
    11281 tttcgcgccg tagattttaa cgaagttacc gtattcgtcg atctgcttac gggtcagcga
    11341 tgcgccaccc ggaacacgta gagcggcaac acggcctttc ggatcgttcg ccggccccgc
    11401 aaatactgca aactcaacag atttcagcag atcggcaacg tcggtcagtt ccatcgggtt
    11461 acgcagatcc ggtttatcag aaccataacg gcgttctgct tccgcaaagg tcattaccgg
    11521 gaaatcgccc agatccacgc ccttcacttc cagccacaaa tgacgcacca gcgcttccat
    11581 cacttcacgc acttgcggcg cggtcatgaa agaagtttcc acatcgatct gagtaaattc
    11641 aggctgacga tcagcgcgca ggtcttcgtc acggaagcat ttaacgatct gatagtaacg
    11701 gtcaaaaccg gacatcatca gcagctgttt gaacaactgt ggggattgcg gcagtgcgta
    11761 gaatttacct ttgtgcacac gagaaggcac caagtagtca cgcgcgcctt ccggcgtggc
    11821 tttggtcagc atcggagttt cgatgtcgag gaagccgtgg tcatccataa aacggcgcac
    11881 caggctggtg attttagcgc gggttttcag gcgctgcgcc atttccgggc gacgcaggtc
    11941 gaggtagcgg tatttcagac gcgcttcttc ggtgttgacg tggttagagt caagcggcag
    12001 aacatctgcg cggttgatga tagtcagcga ggacgccagc acttcaattt cgccggtcgc
    12061 catatcgcgg ttaatatttt tttcgtcacg cgcacgtacg gtgcccgtaa cctgaatgca
    12121 gaactcatta cgcagttcag aggccagctt taacgcgtcc gcacgatccg gatcgaaaaa
    12181 tacctgcacg ataccttcgc ggtcgcgcat atcgatgaag atcaggctac caagatcacg
    12241 acgacggttg acccaaccac acagagtcac ctgctgcccc acgtgggaca aacggagctg
    12301 tccacaatat tctgtacgca tgagatatcc cttaacttag ctgccggcgg atgccccctg
    12361 ctgcgcaggt gaccaagtcg cagcgttagc tgtatgtcac aactgaatga aaaaaggcgg
    12421 ctattatact ggaaattctg ccgcaccgta agagcctggc ccgcgctgta acgcctcgtt
    12481 accactttat atcgggcctg aaatcagact ctacgccagt ttgctataaa ggtgttgccc
    12541 gaactcataa aaattaacaa aatttgtcgt tccgccatcg gctaatcgca ttaaggtgtg
    12601 aggcacgatt ttgttttttc aggagtcagc atgcttgaac ttaatgctaa aaccactgcg
    12661 ttggtggtga tcgatttaca agaaggcatc ttaccttttg ccggtggtcc acatactgcc
    12721 gatgaggtag ttaatcgcgc cgggaagctg gcggcgaaat ttcgcgccag cggtcagccc
    12781 gtgtttctgg tgcgagttgg ctggtctgcc gattacgccg aagcattaaa acagccggtt
    12841 gatgccccct cccccgctaa agtgttgccc gaaaactggt ggcaacatcc agctgcatta
    12901 ggtgcaactg acagtgatat cgaaatcatc aaacgtcagt ggggtgcgtt ttacggtacg
    12961 gatctggagt tgcaactgcg ccgccgaggt atcgatacaa tagtgttatg tgggatctcg
    13021 accaatatcg gtgttgaatc caccgcccgc aatgcctggg aacttggctt taatctggtg
    13081 attgccgaag acgcctgtag tgccgccagc gccgagcaac acaataacag cattaatcat
    13141 atctacccgc gcatcgcccg tgtgcgtagc gtggaagaga tccttcacgc gttatgattt
    13201 acatcggtct accgcaatgg tcgcatccta aatgggtgcg gttggggatc accagccttg
    13261 aagagtatgc ccgccacttt aactgcgtga tacgggattt ttaaaaatca ctaaagaacg
    13321 cccaagagca tgtgttttct ttagaataat caaagcgttg gattgccatg ccatatgaaa
    13381 atcggcaaag ttcatatgta acaaagtgtc ccaatcatgc cccaaaatgc cccacaacaa
    13441 atatttttgc cccatgcatg ccccaaaaaa tcactttccc tcactcttga cactgtttat
    13501 ccatacagtt aaaaataata ctgtataaaa acacagtgta gagggatttt tatgcgtatt
    13561 gaaatctgca tagccaaaga gaaaatgact aaaatgccaa ccggtgctgt ggatgcgtta
    13621 aaggaagaat taacccgacg catcagtaaa cgttatgacg atgtagaggt gatcgtaaaa
    13681 gccaccagca acgatggcct ttctgttacg cgcaccgccg ataaagattc agctaaaact
    13741 tttgttcagg aaactctgaa agatacctgg gagtctgctg acgagtggtt tgttcactaa
    13801 ttagcacgta aaatcagtaa cggctggaaa tcattcaata ctcgcactat cggaagttca
    13861 ccagccagcc gcagcacgtt cttgcatacg acgtgtctgc ggtctcaact ccgcccgccc
    13921 ccgccaatca tgattgggca gtaccagtac agcactagca aagacaggaa attagcagtc
    13981 tcagcaaaac accgaccaga cggtgagaag acaaaaagat acgcaaagat gccgcggttc
    14041 cagaagaata aaaaaccgct aatggacagc ctccttaaca cagtttttta tatacatgat
    14101 tcactgatac cagtggcatt aatagctatt atatagcttc agctcctttt ttcatacctt
    14161 aaggaaaaat tgccccctcc cccaaaaagt atcgtttttt tacattaaac cgatccagtt
    14221 taggatagtg taaagcagaa gttcttcaat atataacata tggagttaat atgcttacac
    14281 aaactatata ttcgcctcct ccagaagttc ctgtctactg ctgaagggaa ccaataacat
    14341 tgcaaagcag ataaaaaaga atccaacacc tctcaacata aagaatacgc cctgatattt
    14401 atgctcccga caaataacaa tcacagatta atttcaaatt cgttctccac ttattcaatc
    14461 gacactagcc gtgcatatga aaattatcta acccattgga ctgaatggaa aaataaccgc
    14521 atacaagaag aacaacgaga catcgctttt cagcgactag tatcatgtct acaaaaccaa
    14581 gagacgaacc tggacttgtc tgaattaggc ctgacaacat tacctgaaat ccccccggaa
    14641 attaaatcaa ttaatataag taaaaataat ttaagcttaa tctccccatt gcctgcgtcc
    14701 cttacacagc ttaatgtcag ctataacaga cttattgaac tgcctgcttt gcctcaagga
    14761 cttaaattat tgaatgcgtc ccacaatcaa ctaatcacac tacccacact ccccatatct
    14821 ttgaaggagc ttcatgtctc aaataatcaa ttatgttctc ttcctgtttt accagaacta
    14881 ctggaaacat tagatgtatc atgtaatggg ctggcagttt taccaccttt accattttct
    14941 ttacaagaga ttagcgcaat agggaatctt cttagtgaac tcccccctct acctcacaac
    15001 attcactcca tatgggcaat cgacaatatg ttaaccgata ttccatacct gccggaaaat
    15061 ttaaggaacg gttattttga cataaatcag ataagtcata tcccggaaag cattcttaat
    15121 ctgaggaatg aatgttcaat agatattagt gataacccat tgtcatccca tgctctgcaa
    15181 tccctgcaaa gattaacctc ttcgccggac taccacggcc cgc
//
LOCUS       DAJVQG010000098        15074 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJVQG010000098 DAJVQG010000000
VERSION     DAJVQG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15074
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.098"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..771
                     /locus_tag="OQM83_002721"
     CDS             <1..771
                     /locus_tag="OQM83_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1520944.1"
                     /translation="SLFFMSVMELRDLLIALESRLHQKHKLNAAARLQYEQARNKVIK
                     KMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLHDGSKCELVVNELQIE
                     MLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFD
                     HYLAVLYRFKDESGKEQFSGTVVKTREATPGKEIEAITRRMLDFSPRLKKLAGVPCQV
                     YVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK"
     gene            complement(974..1327)
                     /locus_tag="OQM83_002722"
     CDS             complement(974..1327)
                     /locus_tag="OQM83_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCS1520945.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1465..3111)
                     /gene="groL"
                     /locus_tag="OQM83_002723"
     CDS             complement(1465..3111)
                     /gene="groL"
                     /locus_tag="OQM83_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCS1520946.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3155..3448)
                     /locus_tag="OQM83_002724"
     CDS             complement(3155..3448)
                     /locus_tag="OQM83_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCS1520947.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3724..4980
                     /gene="yjeH"
                     /locus_tag="OQM83_002725"
     CDS             3724..4980
                     /gene="yjeH"
                     /locus_tag="OQM83_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCS1520948.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5002..5472)
                     /gene="fxsA"
                     /locus_tag="OQM83_002726"
     CDS             complement(5002..5472)
                     /gene="fxsA"
                     /locus_tag="OQM83_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCS1520949.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            5809..7245
                     /gene="aspA"
                     /locus_tag="OQM83_002727"
     CDS             5809..7245
                     /gene="aspA"
                     /locus_tag="OQM83_002727"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCS1520950.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7363..8664
                     /gene="dcuA"
                     /locus_tag="OQM83_002728"
     CDS             7363..8664
                     /gene="dcuA"
                     /locus_tag="OQM83_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCS1520951.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8780..9118
                     /gene="cutA"
                     /locus_tag="OQM83_002729"
     CDS             8780..9118
                     /gene="cutA"
                     /locus_tag="OQM83_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCS1520952.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9094..10791
                     /gene="dsbD"
                     /locus_tag="OQM83_002730"
     CDS             9094..10791
                     /gene="dsbD"
                     /locus_tag="OQM83_002730"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCS1520953.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10828..11403
                     /locus_tag="OQM83_002731"
     CDS             10828..11403
                     /locus_tag="OQM83_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1520954.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11510..11585
                     /locus_tag="OQM83_002732"
     tRNA            11510..11585
                     /locus_tag="OQM83_002732"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11543..11545,aa:Phe,seq:gaa)
     gene            12202..13739
                     /gene="cadC"
                     /locus_tag="OQM83_002733"
                     /pseudo
     CDS             12202..13739
                     /gene="cadC"
                     /locus_tag="OQM83_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
     gene            14104..>15074
                     /gene="cadB"
                     /locus_tag="OQM83_002734"
     CDS             14104..>15074
                     /gene="cadB"
                     /locus_tag="OQM83_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HCS1520955.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT         3701 a         3763 c         3615 g         3995 t
ORIGIN      
        1 tcgttgttct ttatgtctgt catggaactg cgcgacctgc tgattgcgct ggaaagtcgt
       61 ctgcatcaaa agcataaact gaatgctgct gcacgccttc agtatgagca agctcgcaat
      121 aaagtcatta agaaaatggc agaaaacatc ccagaaattc tggttgatga gcttaaaaat
      181 gccgatatta atcgtcgggt taacacgctg gaacttaccg ataatcaagg cgaaaatctt
      241 accttcgtat taacgctaca tgacggtagc aaatgtgagt tagtcgtcaa tgagttgcag
      301 attgaaatgc tggcgcgagc aatcattcat gcaattaata atgcggaaat gcgtgaactg
      361 gcattacgta ttacctcact gttagatttc ctgccgttat acgacgttga ctgccaggac
      421 aatggcaatc tggaatatga cacctattcc caaccggaat ggaaacataa cctgttcgac
      481 cattatctgg cggtgcttta ccgctttaaa gatgaaagtg gcaaagaaca atttagcggc
      541 acagtggtga aaacgcgtga agcaacgccg ggtaaagaaa tagaggcgat cactcgccga
      601 atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg tgccatgtca ggtctatgtt
      661 cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg atcaatgctt acgcgcgctg
      721 catcacttac gtgttcaatc caccagcaaa actgcaccac aggcgaaata atctcatctt
      781 tgctaatgcg aggctggagc catccagcct gaaatgactg ataacgtgat cgttggtcat
      841 gccggatgcg acgtaaacgc cttatccggc ctacaaattc tcaagcattc aatcagttgc
      901 aatgcctgct gtaggcctga taagcgaagc gcatcaggca ataatacgtt tgtcacccag
      961 cctctggatc acatcagttc gggcacttat aaacctgacc gataatctgg ctatccgtcg
     1021 ggacaaaact ggacaacatt ccctgcgacg ggctgctgat gccataaatc acgttaccgc
     1081 ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc gcgcatagaa ccgccctctt
     1141 ctccgtgttg cccggaaagc cagttgcttt gcttacctgt cgcagtacca atcagctggc
     1201 actctgcgcc tggctgctcg tccacaatgc gtacactctg accggcagca ctcaattcgt
     1261 tgctggagct acaacccgcc atcagtagcg cggcaccgac aatccctgct aagtatttta
     1321 cgtgcatgtt attccccata atcaatgagc tggacgccac tcgcgtcgtc cgtgtctgaa
     1381 tcttatacta gaaagatgac caaaagaaaa acccccagac atttctgccc gggggtttgt
     1441 ttatttctgc gaggtgcagg gcaattacat catgccgccc atgccaccca tgccgcccat
     1501 accgccagca gcgcctaagt cagctgcatc atttttcggc aggtcggtaa ccatgcactc
     1561 ggtggtgatc atcaggccag ccacagaagc cgcgtactgc agagcagaac gggttacttt
     1621 ggttgggtcc aggataccca tgtcgatcat gttgccgtat tcttcggttg ctgcgttgta
     1681 accgtagttg ccgtcgccgc ctttaacggt gttagcaaca acagacggtt cttcgccgca
     1741 gttcaggacg atctgacgca gcggagcttc cattgcacgc agtgcaactt tgatacccac
     1801 gttctggtct tcgttctgac cacgcaggtc agccagttta gacgctacgc ggatcagcgc
     1861 aacaccacca ccagcaacca cgccttcttc taccgcagca cgggtcgcgt gcagggcgtc
     1921 ttcaacgcgt gcttttttct ctttcatttc aacttcggta gcagcaccta ctttgataac
     1981 tgcaacgccg cctgccagtt tcgctacgcg ttcctgcagt ttttcacggt cgtagtcaga
     2041 agttgcttct tcaatctgct gacggatctg agcaacacgg ccctggattg cagcttcttc
     2101 acccacgcca tcgatgatag tggtggtgtc tttgttgatc acaacacgtt tagcctgacc
     2161 caggtcttcc agggttgctt tttccagttc cataccgatc tcttcagaga tcacggtacc
     2221 gccagtcagg gttgcgatat cctgcagcat agctttacga cgatcgccga agcccggagc
     2281 tttaactgca gcaactttca cgatgccacg catggtgtta acaaccagag ttgccagcgc
     2341 ttcgccttct acatcttcag cgatgatcag cagcggtttg cctgctttgg caacggcttc
     2401 cagaaccggc agcatttcgc ggatgttgga gattttcttg tcagccagca ggatgaacgg
     2461 gctttccagt tctactgcgc cagtttccgg cttgttgatg aagtaaggag acaggtagcc
     2521 acggtcgaac tgcatacctt caaccacgtc cagttcgtcc tgcagaccgg taccgtcttc
     2581 aacggtgata acgccttctt taccgacttt gtccatcgct tcagcgatca gtttacctac
     2641 ggtttcgtcg gagttagcgg agatggtacc aacctgagca atcgctttag agtcagagca
     2701 cggtacggac agcgctttca gttcttcaac tgcagcggta acggctttgt cgataccacg
     2761 tttcaggtcc atcgggttca tgcccgcagc aacagctttc agaccctcag tgatgatagc
     2821 ctgagccagt acagttgcag tggtggtacc gtcgcctgca gcgtcgttcg ctttagaggc
     2881 aacttctttc accatctgcg cacccatgtt ttcgaacttg tcttccagtt cgatttcacg
     2941 agcaacggaa acaccatctt tggtgatggt cggtgcaccg aaagatttat ccagaactac
     3001 gttacggcct ttcggaccga gggtaacttt cactgcatct gccagtacgt ttacgccgcg
     3061 cagcattttc acacgagcgt cgttaccgaa ttttacgtct ttagctgcca ttatctttat
     3121 tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc ttcaacaatt gccagaatgt
     3181 cgctttcgga catgatcaac acttcttcat tgtcgatctt ctcagatttc acaccgtagc
     3241 catcgttgaa aataacgatg tcgccaactt tcacatccag cggcttcact tcgccatttt
     3301 caaggatacg gccattgccg acagccagca cttcgccgcg ggtggattta gccgctgcag
     3361 agccggtcag aacgatgccg ccagcagatt tagtttcaac ttctttacgc ttgacgatca
     3421 cgcgatcatg caatggacga atattcattg ataactctcc tttgagaaag tccgtatctg
     3481 ttatgggtga cgccggagct tacgtggttt cccggctggt gaccagagaa atggggatga
     3541 ggattcgccc cttcaagggg gaaaaagaaa aaaaattctg atcagcacaa aatcgggtga
     3601 aaaccctgat tcacctcaca tttcatcgca atttcttcat cgccgtcata agcgaatctg
     3661 attgtgctac catcgaaaat ctacgcagtt gcccaaaatt tgggcgcaat cggatatcaa
     3721 cccatgagtg gactcaaaca agaactgggg ctggcccagg gcattggcct gctatcgacg
     3781 tcattattag gcaccggcgt gtttgccgtt cctgcgttag ctgcacaggt agcaggcaat
     3841 aacagcctgt gggcgtggcc cgttttgatt atcttagtgt tcccgattgc gattgtgttt
     3901 gcgattctgg gtcgccacta tcccagcgca ggcggtgtcg cgcacttcgt cggtatggcg
     3961 tttggttcgc ggcttgagcg agtcaccggc tggttgtttt tatcggtcat tcccgtgggt
     4021 ttgcctgccg cgctacaaat tgccgccggg ttcggccagg caatgtttgg ctggcatagc
     4081 tggcaactgt tgttggcaga actcggtacg ctggcgctgg tgtggtatat cggtactcgc
     4141 ggtgccagtt ccagtgctaa tctacaaacc gttattgccg gacttatcgt cgcgctgatt
     4201 gtcgctatct ggtgggcggg cgatatcaaa cctgcgaata tccccttccc ggcacctggt
     4261 aatatcgaac ttaccgggtt atttgctgcg ttatcagtga tgttctggtg ttttgtcggt
     4321 ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc cagagcgtga ttttcctcgc
     4381 gcattgatga ttggtctgct gctggcagga ttagtctact ggggctgtac ggtagtcgtc
     4441 ttacacttcg acgcctatgg tgaacaaatg gccgcggcag catcgcttcc aaaaattgta
     4501 gtgcaactgt tcggtgtagg agcgttatgg attgcctgcg taattggcta tctgacctgc
     4561 tttgccagtc tcaacattta tatacagagc ttcgcccgcc tggtctggtc gcaggcgcaa
     4621 cataatcctg accactacct ggcacgcctc tcttctcgcc atatcccgaa taatgccctc
     4681 aatgcggtgc tcggctgctg cgtggtgagc actttggtga ttcatgcttt agagatcaat
     4741 ctggacgctc ttattattta tgccaatggc atctttatta tgatttatct gttatgcatg
     4801 ctggcaggct gtaaattatt gcaaggacgt tatcgactac tggcggtggt tggcgggctg
     4861 ttatgcgttc tgttactggc aatggtcggc tggaaaagtc tctatgcgct gattatgctg
     4921 gcggggttat ggctgtttct gccaaaacga aaaacgccgg aaaatggcat aaccacataa
     4981 tccggcgttt cgacattaat cctagcgatc gtctttatga tcaaggcggt cgcggtcatc
     5041 atcctttcgc tggtactcac catcaaaagt attaccgcca ccggtcccgg cgctaaaacc
     5101 gccgcctggc atgcgagaaa agcgcaaatg tggcatcaac ttcactgtca gatgcttttg
     5161 caccggcggc aataaaagca gaagaccgag gaagtcggtg aaaaagcccg gtaataaaag
     5221 cagcaaaccg gcaataatca gcgaaacact tttaatcatc tccgccgcag ggttttcacc
     5281 cgccgccatt ttttgctgca tcagcacgaa attcttaaag ccctggttac gtaccagtga
     5341 cataccgata actgacgtga atataaccag cacgagggtc agcaataccc ccaatacatg
     5401 ggcaacctga ataaagattg aaatctcaat atagacataa aggaaaatgg caataaaagg
     5461 taaccagcgc aaaggtttct cctgtaatag cagccggtta aacccggcta cctgaatggg
     5521 ttgcgaatcg cgttcagctt atattgtggt cattagcaaa atttcaagat gtttgcgcaa
     5581 ctatttttgg tagtaatccc aaagcggtga tctatttcac aaattaataa ttaaggggta
     5641 aaaaccgaca cttaaagtga tccagattac ggtagaaatc ctcaagcagc atatgatctc
     5701 gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa acattcgaaa ccacatatat
     5761 tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga aggttcacat gtcaaacaac
     5821 attcgtatcg aagaagatct gttgggtacc agggaagttc cagctgatgc ctactatggt
     5881 gttcacactc tgagagcgat tgaaaacttc tatatcagca acaacaaaat cagtgatatt
     5941 cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg cagctatggc aaacaaagag
     6001 ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg ccgcatgtga tgaagtcctg
     6061 aacaacggaa aatgcatgga tcagttcccg gtagacgtct accagggcgg cgcaggtact
     6121 tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg gtctggaact gatgggtcac
     6181 cagaaaggtg aatatcagta cctgaacccg aacgaccatg ttaacaaatg tcagtccact
     6241 aacgacgcct acccgaccgg tttccgtatc gcagtttact cttccctgat taagctggta
     6301 gatgcgatta accaactgcg tgaaggcttt gaacgtaaag ctgtcgaatt ccaggacatc
     6361 ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga tgaccctcgg tcaggaattc
     6421 cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca tccaacgtac cgctgaactg
     6481 ctgctggaag ttaaccttgg cgcaacagca atcggtactg gtctgaacac gccgaaagag
     6541 tactctccgc tggcagtgaa aaaactggct gaagtcactg gcttcccatg cgtaccggct
     6601 gaagacctga tcgaagcgac ctctgactgc ggcgcttatg ttatggttca cggcgcgctg
     6661 aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc tgcgcttgct ctcttctggt
     6721 ccacgtgccg gcctgaacga gatcaacctg ccggaactgc aggcgggctc ttccatcatg
     6781 ccagctaaag taaacccggt tgttccggaa gtggttaacc aggtatgctt caaagtcatc
     6841 ggtaacgaca ccactgttac catggcagca gaagcaggtc agctgcagtt gaacgttatg
     6901 gagccggtca ttggccaggc tatgttcgaa tccgttcaca ttctgaccaa cgcttgctac
     6961 aacctgctgg aaaaatgcat taacggcatc actgctaaca aagaagtgtg cgaaggttac
     7021 gtttacaact ctatcggtat cgttacttac ctgaacccgt tcatcggtca ccacaacggt
     7081 gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg tacgtgaagt cgttctggaa
     7141 cgcggtctgt tgactgaagc ggaacttgac gatattttct ccgtacagaa tctgatgcac
     7201 ccggcttaca aagcaaaacg ctatactgat gaaagcgaac agtaatcgta cagggtagta
     7261 caaataaaga aggcacgtca gatgacgtgc cttttttctt gtgagcagta acttaaaaat
     7321 aacaatctaa tatcaacttg ttaaaaaaca aggaaggcta atatgctagt tgtagaactc
     7381 atcatagttt tgctggcgat cttcttgggc gccagattgg ggggaatagg tattggtttt
     7441 gcaggcggat tgggggtgct ggttcttgcc gctattggcg ttaaacccgg taacatcccg
     7501 ttcgatgtca tttccattat catggcggtt atcgccgcta tttctgccat gcaggttgct
     7561 ggcggtctgg actatctggt tcatcagaca gaaaagctgc tgcgccgtaa cccgaaatac
     7621 atcacaatcc tcgcaccgat cgtgacctat ttcctgacaa tctttgctgg tactggcaac
     7681 atctctctgg cgacactgcc agttatcgct gaagttgcga aggaacaagg cgttaaacct
     7741 tgccgtccgc tgtctactgc agtggtatcc gcgcagattg cgatcaccgc atcgccaatc
     7801 tcagcggcag tggtttacat gtcttccgtg atggaaggtc atggcatcag ctacctccat
     7861 ctgctctccg tggtcatccc gtccaccctg ctggcggttc tggtgatgtc cttcctggtc
     7921 actatgctgt tcaactccaa actctctgac gatccgattt atcgcaagcg tctggaagag
     7981 ggcctggttg aactgcgcgg tgaaaagcag attgaaatca aatccggtgc aaaaacgtcc
     8041 gtctggctgt tcctgctggg cgtagttggc gtggttatct atgcaatcat caacagccca
     8101 agcatgggtc tggttgaaaa accgctgatg aacaccacca acgcaatcct gatcatcatg
     8161 ctcagcgttg caactctgac caccgttatc tgtaaagtcg ataccgacaa catcctcaac
     8221 tccagcacct tcaaagcagg tatgagcgcc tgtatttgta tcctgggtgt tgcgtggctg
     8281 ggcgatactt tcgtttccaa caacatcgac tggatcaaag ataccgctgg tgaagtgatt
     8341 cagggtcatc cgtggctgct ggccgtcatc ttcttctttg cttctgctct gctgtactct
     8401 caggctgcaa ccgcaaaagc actgatgccg atggctctgg cactgaacgt ttctccgctg
     8461 accgctgttg cttctttcgc tgcggtgtct ggtctgttca ttctgccgac ctatccgacg
     8521 ctggttgctg cggtacagat ggatgacacg ggtacaaccc gtatcggtaa attcgtcttc
     8581 aaccatccgt tcttcatccc gggtactctg ggtgttgccc tggccgtttg cttcggcttc
     8641 gtgctgggta gcttcatgct ataatgacca atcgcggggc gttcacgccc cgctttcttt
     8701 cccgccgact aacatccttc ccccgtccgt tgtatagtga cctctctctt gcggttccat
     8761 ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg aataccgcgt ctgtcgtggt
     8821 gctatgtacc gcaccggatg aagcgacagc ccaggattta gccgccaaag tgctggcgga
     8881 aaaactggcg gcctgcgcga ccttgatccc cggcgctacc tctctctatt actgggaagg
     8941 taagctggag caagaatacg aagtgcagat gattttaaaa actaccgtat ctcaccagca
     9001 ggcactgctg gaatgcctga agtctcatca tccatatcaa accccggaac ttctggtttt
     9061 acctgttaca cacggagaca cagattacct ctcatggctc aacgcatctt tacgctgatc
     9121 ctgctacttt gcagcacttc cgtttttgcc ggattattcg acgcgccggg acgttcacat
     9181 tttgtccccg cggatcaagc ctttgctttt gattttcagc aaaaccaaca tgacctgaat
     9241 ctgacctggc agatcaaaga cggttactac ctctaccgta aacagatccg cattacgccg
     9301 gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg tctggcatga agatgagttt
     9361 tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc ccgtaaccat caaccaggcg
     9421 agtgcgggag cgacgttaac tgtcacctac cagggctgtg ctgatgccgg tttctgttat
     9481 ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg ccaacaacgc agcgtcacag
     9541 cctgtgtctg ttccgcagca agagcagcac accgcgcaat tgcccttttc cgcgctctgg
     9601 gcgttgttga tcggtattgg tatcgccttt acgccatgcg tgctgccaat gtacccactg
     9661 atttctggca tcgtgctggg tggtaaacag cggctctcca ctgccagagc attgttgctg
     9721 acctttattt atgtgcaggg gatggcactg acttacacgg cgctgggtct ggtagttgcc
     9781 gccgcagggt tacagttcca ggcggcgcta cagcacccat acgtgctcat tggcctcgcc
     9841 atcgtcttta ccttgctggc gatgtcaatg tttggcttgt ttactctaca actcccctct
     9901 tcgctgcaaa cacatctcac attgatgagc aatcgccaac agggcggctc acctggcggt
     9961 gtgtttgtta tgggggcgat tgccggactg atctgttcac catgcaccac cgcaccgctt
    10021 agcgcgattc tgctgtatat cgcccaaagc gggaacatgt ggctgggcgg cggcatgctt
    10081 tatctctatg cgttgggcat gggcctgccg ctgatgctaa ttaccgtctt tggtaaccgc
    10141 ttgctgccga aaagcggccc gtggatggaa caagtcaaaa ccgcgtttgg ttttgtgatc
    10201 ctcgcactgc cgatcttcct gctggagcga gtgattggtg atatatgggg attacgcttg
    10261 tggtcggcgc tgggtgtcgc attctttggc tgggccttta tcaccagcct acaggccaaa
    10321 cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag cggcattggt tagcgtgcgc
    10381 ccacttcagg attgggcatt tggtgcgacg cataccgcgc aaactcagac gcatctcaac
    10441 tttacacaaa tcaaaacggt agatgagtta aatcaggcgc tcgttgaagc caaaggcaaa
    10501 ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct gtaaaaagtt tgagaaatac
    10561 accttcagcg acccgcaggt gcaaaaagcg ttagcagaca cggtcttact tcaggccaac
    10621 gtcactgcta acgacgcaca agatgtggcg ctgttaaagc atcttaatgt ccttggacta
    10681 ccgacaattc tcttttttga cggacaaggc caggagcatc cacaagcacg cgtcacgggc
    10741 tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc gccaaccgtg aacgacactt
    10801 gcagtgggaa agacggagga aaatagcgtg caacgtgaag atgtactggg agaagccctg
    10861 aaattattag aattacaagg gattgccaac accacgctgg agatggttgc tgaacgtgtg
    10921 gattatccac tggacgagct acgccgcttc tggccagaca aagaggcaat cctctacgat
    10981 gcgctgcgtt atcttagcca acagatagat gtctggcgtc gtcagctgat gcttgacgaa
    11041 acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg cgttatcgga gtgcgttaaa
    11101 aacaaccgct atccgggctg tctatttatc gctgcctgta cgttttatcc cgaccctggc
    11161 caccctattc atcaactggc cgatcagcaa aaaagcgcgg cctacgattt cacccacgaa
    11221 ctgttaacca cgctggaagt tgacgatccg gcgatggtag caaagcagat ggaactggtg
    11281 ctggaaggct gtttaagccg aatgctggtg aatcgtagcc aggcggatgt cgacaccgca
    11341 catcggctgg cggaagatat cctgcgcttc gcccgctgcc gccagggtgg tgcactgacc
    11401 tgacagaaac acagaaaaga agcgatttgc cgcaatctta agcagttgaa tcgcttttac
    11461 tgaaattagg ttgacgagat gtgcagatta cggtttaatg cgccccgttg cccggatagc
    11521 tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt ggttcgattc cgagtccggg
    11581 caccaaattc atataaacgg acctccacgg aggtccgttt ttcgtttcag gacgccacga
    11641 tttaagcgtt ctgcctccaa atcaattcta ccgaactcaa ccagattctc cccacatcac
    11701 cagcattttg cgggtatatc ccaattcggg aaaatttgtt cctgagcgat agcactgact
    11761 gacgtgaaat gtcgtgcggc cccgtgatgt tgttgaacgt caaatgacgt catcaggagc
    11821 gtaacgcatc cataaagcac aacaccggtc tgaacggcga ctgatgagat tttctgaatg
    11881 agaacaaaga gaaatgtatc agtccgcttg ctcatgcaaa gactaacaat ccattaaaat
    11941 agtaagcgct ccggacaatt ttccatggat tattttctac acatttttct ttggcaaaga
    12001 tgatgaattt tgatggtaag gaatattact tctggttctc agtaaaatcc tttcgtaata
    12061 ctatgtaatc aagaagttta tggctggtaa aaataacgtc ttgcattcac caataacatg
    12121 taaataaacc catctattga tggtaaaaat aggtcatggc aattatcatt gcaccattcc
    12181 cttttcgaat gagtttctat tatgcaacaa cctgtagttc gcgttggcga atggcttgtt
    12241 actccgtcca taaaccaaat tagccgcaat gggcgtcaac ttacccttga gccgagatta
    12301 attgatcttc tggttttctt tgctcaacac agtggcgaag tacttagcag ggatgaactt
    12361 atcgataatg tctggaagag aagtattgtc accaatcacg ttgtgacgca gagtatctca
    12421 gaactacgta agtcattaaa agataatgat gaagatagtc ctgtctatat cgctactgta
    12481 ccaaagcgcg gctataaatg aatggtgccg gttatctggt acagcgaaga agagggagag
    12541 gaaataatgc tatcttcgcc tccccctata ccagaggcgg ttcctgacac aaattctccc
    12601 tcccacagtc ttaccattca aaacaccgca acgccacctg aacaatcccc agttaaaagc
    12661 aaacgattca ctaccttttg ggtatggttt ttttcctgtt gtcgttaggt atctgtgtag
    12721 cactggtagc gttttcaact cttgatacac gtcttcctat gagcaaatcg cgtattttgc
    12781 tcaatccacg cgatattgac attaatatgg taaataaaag ttgtaacagc tggagttccc
    12841 cgtatcagct ctcttacgcg ataggcgtgg gtgatttggt ggcgacatca cttaacacct
    12901 tctccacctt tatggtgcat gacaaaatca actacaacat tgatgaaccg agcagttccg
    12961 gtaaaacatt atctattgcc tttgttaatc agcgccaata ccgtgctcaa caatgcttta
    13021 tgtcggtaaa attagtagac aatgcagatg gttcaaccat gctggataaa cgttatgtca
    13081 tcactaacgg taatcagctg gcgattcaaa atgatttact ggagagttta tcaaaagcgt
    13141 taaaccaacc gtggccacaa cgaatgcagg agacgctcca gcaaattttg cctcatcgtg
    13201 gtgcgttatt aactaatttt tatcaggctc atgattattt actgcatggc gatgacaaat
    13261 cattgaatcg tgccagtgaa ttattaggtg agattgttca atcatcccca gaatttacct
    13321 acgcgagagc agaaaaagca ttagttgata tcgtgcgcca ttctcaacac cctttagatg
    13381 aaaaacaatt agcagcactg aacacagaaa tagacaacat tgttacactg ccggaattga
    13441 acaacctgtc cattatatat caaataaaag cggtcagtgc tctggtaaaa ggtaaaatag
    13501 atgagtctta ccaggcgata aatactggca ttgatcttga aatgtcctgg ctaaattatg
    13561 tgttgcttgg caaggtttat gaaatgaagg ggatgaaccg ggaagcagct gatgcatatc
    13621 tcaccgcctt taatttacgc ccaggggcaa acacccttta ctggattgaa aatggtattt
    13681 tccagacttc tgttccttat gttgtacctt atctcgacaa atttctcgct tcagaataag
    13741 taactccggg ttgatttatg ctctggaata tttgttgttg agtttttgta tgttcccatt
    13801 ggtataatat gttgcggcaa tttatttgcc gcataatttt tattacataa atttaaccag
    13861 agaatgtcat gcaatccatt gtaaacattg aatgtttatc ttttcatgat atcaacttgc
    13921 gatcctgatg tgttaataaa aaacctcaag ttctcactta cagaaacttt tgtgttattt
    13981 cacctaatct ttaggattaa tccttttttc gtgagtaatc ttatcgccag tttggtctgg
    14041 tcaggaaata gttatacatc atgacccgga ctccaaattc aaaaatgaaa ttaggagaag
    14101 agcatgagtt ctgccaagaa gatcgggcta tttgcctgta ccggtgttgt tgccggtaat
    14161 atgatgggga gcggtattgc attattacct gcgaacctag caagtatcgg tggtattgct
    14221 atctggggtt ggattatctc tattattggt gcaatgtcgc tggcgtatgt atatgcccga
    14281 ctggcaacaa aaaacccgca acaaggtggc ccaattgctt atgccggaga aatttctcct
    14341 gcatttggtt ttcagacagg tgttctttat taccatgcta actggattgg taacctagcg
    14401 attggtatta ccgctgtatc ttatctttcc accttcttcc cagtattaaa tgatcctgtt
    14461 ccggcgggta tcgcctgtat tgctatcgtc tgggtattta cctttgtaaa tatgctcggc
    14521 ggtacctggg taagccgttt aaccactatt ggtctggtgc tggttcttat tcctgtggtg
    14581 atgactgcta ttgttggctg gcattggttt gatgcggcaa cttatgcagc taactggaat
    14641 actgcggata ccactgatgg tcatgcgatc attaaaagta ttctgctcta cctgtgggcc
    14701 ttcgtgggtg ttgagtccgc agcagtaagt actggtatgg ttaaaaaccc gaaacgtacc
    14761 gttccgctgg caaccatgct gggtactggt ttagcaggta ttgtttacat cgctgcgact
    14821 caggtgcttt ccggtatgta tccgtcttct gtaatggcgg cttccggtgc tccgtttgca
    14881 atcagtgctt caactatcct cggtaactgg gctgcgccgc tggtttctgc attcaccgcc
    14941 tttgcgtgcc tgacttctct gggctcctgg atgatgttgg taggccaggc aggtgtacgt
    15001 gccgctaacg acggtaactt cccgaaagtt tatggtgaag tcgacagcaa cggtattccg
    15061 aaaaaaggtc tgct
//
LOCUS       DAJVQG010000099        14995 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJVQG010000099 DAJVQG010000000
VERSION     DAJVQG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14995
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.099"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            258..347
                     /locus_tag="OQM83_002735"
                     /pseudo
     CDS             258..347
                     /locus_tag="OQM83_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(348..1406)
                     /gene="modC"
                     /locus_tag="OQM83_002736"
     CDS             complement(348..1406)
                     /gene="modC"
                     /locus_tag="OQM83_002736"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCS1520956.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1409..2098)
                     /gene="modB"
                     /locus_tag="OQM83_002737"
     CDS             complement(1409..2098)
                     /gene="modB"
                     /locus_tag="OQM83_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCS1520957.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2098..2871)
                     /gene="modA"
                     /locus_tag="OQM83_002738"
     CDS             complement(2098..2871)
                     /gene="modA"
                     /locus_tag="OQM83_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1520958.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3037..3186)
                     /gene="acrZ"
                     /locus_tag="OQM83_002739"
     CDS             complement(3037..3186)
                     /gene="acrZ"
                     /locus_tag="OQM83_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCS1520959.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3315..4103
                     /gene="modE"
                     /locus_tag="OQM83_002740"
     CDS             3315..4103
                     /gene="modE"
                     /locus_tag="OQM83_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCS1520960.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4171..5643
                     /gene="modF"
                     /locus_tag="OQM83_002741"
     CDS             4171..5643
                     /gene="modF"
                     /locus_tag="OQM83_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCS1520961.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            5904..6920
                     /gene="galE"
                     /locus_tag="OQM83_002742"
     CDS             5904..6920
                     /gene="galE"
                     /locus_tag="OQM83_002742"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCS1520962.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            6930..7976
                     /gene="galT"
                     /locus_tag="OQM83_002743"
     CDS             6930..7976
                     /gene="galT"
                     /locus_tag="OQM83_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCS1520963.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            7980..9128
                     /gene="galK"
                     /locus_tag="OQM83_002744"
     CDS             7980..9128
                     /gene="galK"
                     /locus_tag="OQM83_002744"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCS1520964.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9122..10162
                     /gene="galM"
                     /locus_tag="OQM83_002745"
     CDS             9122..10162
                     /gene="galM"
                     /locus_tag="OQM83_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCS1520965.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10365..11117
                     /gene="gpmA"
                     /locus_tag="OQM83_002746"
     CDS             10365..11117
                     /gene="gpmA"
                     /locus_tag="OQM83_002746"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCS1520966.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11276..12328)
                     /gene="aroG"
                     /locus_tag="OQM83_002747"
     CDS             complement(11276..12328)
                     /gene="aroG"
                     /locus_tag="OQM83_002747"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCS1520967.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            12644..13024
                     /locus_tag="OQM83_002748"
     CDS             12644..13024
                     /locus_tag="OQM83_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCS1520968.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13138..14079
                     /gene="zitB"
                     /locus_tag="OQM83_002749"
     CDS             13138..14079
                     /gene="zitB"
                     /locus_tag="OQM83_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCS1520969.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14076..14795)
                     /gene="pnuC"
                     /locus_tag="OQM83_002750"
     CDS             complement(14076..14795)
                     /gene="pnuC"
                     /locus_tag="OQM83_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCS1520970.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(14833..>14995)
                     /gene="nadA"
                     /locus_tag="OQM83_002751"
     CDS             complement(14833..>14995)
                     /gene="nadA"
                     /locus_tag="OQM83_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCS1520971.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3787 a         3950 c         3746 g         3512 t
ORIGIN      
        1 aaataccgtg acggacgagg gagggaatgg aaagacattt aaaattaatt tcgaatgatt
       61 tcgaaacacc acttgtaatt aattgattat aaatgattaa ttcctaatgg gcatttgctt
      121 catgaatgct cctttgcatt tagcaactga ctgtaacagc taaaattagt cgcttttggc
      181 ggtgaatgct gaattaaaac gcagaatttt gtccgatagt ggtatcggtg taccattcgc
      241 gcagacgaaa attcttaaat gacgcggtaa aagcgcgcgc caacattgtg attggtgata
      301 acaccaccga cagcattgcc cagttcattt atagccacct gatttaatca ggcggttatc
      361 gacacacttt taatttgcgc gtacagccac aggccaggtt tgatcgccag ttcatccctg
      421 gcccacgggc tgatacgcgc ccacagcgtt ttaccgccga cttccagctc cacttccacc
      481 tggccgttgt cgtcataact attaacaact tttgcccgca atacgttacg aatgctggtt
      541 tgctgcggcg gttgtaaaac cagagaaaca tcggaagcct gaatgcggat acgtagcgca
      601 gcttgcagcg gttcgtccag cttattgacc cacaaatgct gatcgcccag cgccagcgcg
      661 gtcatcgcgt aatgcggatg atgctcaagt accgtcactt tcagaatgct actttgttgc
      721 tctttcggca gccacggatt catcacgcta ctgccccaca cttcctccag cgcgccaaag
      781 gctttcacct gaccgttttc cagtaccatc actctgtctg ccagatggag gatctcatcc
      841 agcgaatggc tgacatacaa catcggaatg ttgatttccc gtgtcagccg ttgcagataa
      901 ggcaacagtt cgcgtttacg cggaatatcc agtgacgcca gcggttcatc cagcaacagc
      961 aattccggtg ctgtcagcaa agcccgacca atcgccacgc gctgtttttc gcctccggac
     1021 aggctgcctg gtaaacggtc aagcaacggt tcaatgccta aaagcgccac cagcttatcg
     1081 aactgatcga ccatactttt cgacatgccg tagcgcagat tgccacgcac tttgtaatgc
     1141 gggaacagcc gcgcatcctg aaaaacatag ccaacgcgac gcttttccgg cgtcaggcag
     1201 ataccttttt cggcatcatt tagtacccgc ccattgagga caatccgccc tttttgcggg
     1261 cgcgtcagtc cactgatggc gttaatcagc gaagtttttc cggcaccgga gacgccaaag
     1321 atagcagtga tgccattggc gggcagtgtt tcattaatgg tcaggcaatg gttgcccaac
     1381 gtctgggaaa aattcagctc cagcatgatt agcgccccgc ccgttcacgg ctgattctgg
     1441 ccagccattc tgaaatcaac agggagatca tcgccagcgc aatagaaata atgcacagtc
     1501 tcgccgcgcc actttccccg ccgggggtct ggatcagggt atacatggca gagggaatgg
     1561 ttcgcgtttc gccaggaatg ttcgacacaa aggtgatggt tgcaccaaac tcaccgagag
     1621 aacgagcaaa agccagtacc gtaccaacaa taattcccgg taaggtcagc ggtaacgtga
     1681 tagtaaagaa aacgcgccag cgcccggcac ctagtgttct tgcggcctgt tcgagtttga
     1741 catcaacccc ttccagcgcc agacgaattg cccgcaccat cagcggaaac gacatgacgg
     1801 cggcagcgag aaccgcgccg cgccagctaa aggcgaaggt aataccaaac cagtcataca
     1861 gacgttcacc gataaatccg cgccgtccca tcgaaactaa taataagtaa ccgacgacca
     1921 cgggcggtaa caccagcggt agatgcagta cgctgtcgag cagagctttg cccggaaacg
     1981 tgcaacgcac cagtaaccag gcaaaaaaga tcccaaacgg caggctaaac agcacagcca
     2041 gggaagaaac tttcaggctt aataaaactg cctgccattc tggatcggtc agtatcatta
     2101 cttggttgta aatccgtaac gtttaaagat ttccgctgcc tgcggtccct tcagataatc
     2161 ataaaatgct ttcactgtcg cattgttatg cccttccaca acagcaaccg gatattccac
     2221 ttttttatgt gaatcttccg ggaaggtggc aaccactttt acccctttgc tgacaactgc
     2281 gtcagaaccg taaacaatgc ccaacggcgc ttcattgcgt tcgaccaacg ccagcgcgcc
     2341 acgaacatct tccgccggag ccagtttcgg ggagagcgta tcccatgcgc ccagtttttg
     2401 cagtgcttct tttgcataaa tgccagcagg gacatgttcc ggatcgccaa ccgcgaggcg
     2461 accgccattc agcagcgaag tccagttagt tgtgctgtcg atgatgaaat ctttctgctc
     2521 gctggctttc ggtgctacaa cgaccaggct attgccgagc agtgtctggc gcgaagccgt
     2581 atcgatcgct tttttatcaa ccgcataatc catccatttc tgatcggcag agataaacag
     2641 atccgcaggc gctcccgctt caatctgacg ggccagagta gatgacgaag cgaaagaaga
     2701 aaccacatcc acgccttttt cttttttata ctgcgtagca atgtcctgca ttgcgttagt
     2761 cagtgatgcg gcggcgaaca ccgtgatttt cccttcatct gccagtgtat tgccagcaac
     2821 agcgaaagag agtgccgccc cggcaaacaa gttcaaccat ttacgagcca ttggtaaccc
     2881 cttaatgtaa cgttatatag gtgacaatgt aacggctagc tcagaatttt cccagcagtt
     2941 attgctaacc tttgtgaggt agataagaaa aaatatcggc tggaaaagca ggaagttgag
     3001 aaaaagaaat gcccgactaa gcgggcattc agggaatcaa tgattttgtc cgggctggtc
     3061 ttttttacca acaccagaaa agatgttgaa tacttcacca agaccgtaaa tcagacccag
     3121 gatgatggcc atcacgacag gtaccatgat tacggcgaat accagacttt ttaataactc
     3181 taacatggtc aactccagat atagtcatga gactattcta accgctaagc acagaaaagc
     3241 actccccttt tgtgcggtca gctttgcgtg gcgttcgttt tccgtcacaa taagactttt
     3301 gccaggacat tgttatgcag gccgaaatcc ttctcaccct taagctccaa caaaaattat
     3361 tcgccgaccc gcgccggatt tcgctactaa aacacattgc gctttccggt tccattagcc
     3421 agggagcgaa agatgccggt atcagctata aaagcgcctg ggatgccatt aacgagatga
     3481 atcagttaag cgagcatatt ctggttgagc gcgcaacagg cggtaaaggt ggcggcggcg
     3541 cagtactgac ccgctatggt cagcgactga ttcagctcta tgacttactg gcgcaaatcc
     3601 agcaaaaagc ctttgatgtg ttaagtgacg atgacgccct gccgctgaac agcctgctgg
     3661 ccgcgatctc acgtttttca ctgcaaacca gcgcccgtaa ccagtggttc ggtaccatca
     3721 ccgcccgcga tcatgatgac attcaacagc atgttgatgt cttactggct gacggaaaaa
     3781 cacgcctgaa agtcgcaatt accgcacaaa gcggcgcacg tctggggctg gatgaaggca
     3841 aagaagtgtt gatattgcta aaagcgccgt gggtaggcat tactcaggac gaagcggtcg
     3901 cacaaaacgc cgacaaccaa ttaccgggta ttattagtca tattgagcgc ggcgcagagc
     3961 agtgcgaagt attaatggcg ctacccgacg ggcaaacact gtgcgccaca gtgccggtaa
     4021 atgaagcgac ttctcttcag caagggcaga atgtcacggc ctactttaat gccgacagcg
     4081 tgattatcgc cacgctgtgc taagcgtgtt gacaatttgt tatgaaacac gtatccctgt
     4141 cagtaatcgc tgcacaaaat gggatataaa atgtcatcgt tgcaaatttt gcaaggcacg
     4201 tttcgtctta gcgacacaaa aacgctgcaa ttgcctcagc taacgttaaa cgcgggtgat
     4261 agttgggcgt ttgtcggttc gaatggaagc gggaaatcgg ccctggcccg cgcgctggcg
     4321 ggggaacttc cgcttttgaa aggtgaacgg caaagccagt tttcccacat cactcgtctc
     4381 tccttcgagc aattgcaaaa actcgtcagc gacgaatggc agcggaataa caccgatatg
     4441 ctcggccctg gcgaagatga caccggacgc actacggctg aaatcattca ggatgaagta
     4501 aaggatgcac cgcgttgcat gcaactggcg cagcagttcg gtattaccgc cctcctcgac
     4561 cgacgcttta aatacctttc cactggcgag acgcgaaaaa ccctgctgtg tcaggcgctg
     4621 atgtcggagc ctgacttgtt gattcttgat gagtcgttcg atggcctgga tgttgcctca
     4681 cgtcagcagc tggctgagcg actcgcctcg ttacatcagt ccggtattac tctggtactg
     4741 gtgctcaatc gcttcgatga gatcccggag tttgtccagt ttgctggcgt gctggcggat
     4801 tgcacgttag cggaaactgg cgctaaagag gaactgctcc aacaagcact cgtcgcgcaa
     4861 ctggcgcata gtgaacagct tgaaggtgtg caactgccgg agccggatga accttcagca
     4921 cgtcacgcct tacccgccaa cgaaccgcgc attgtgctga acaatggcgt ggtttcttat
     4981 aacgatcgcc ccattcttaa taaccttagc tggcaggtga atccaggtga acactggcaa
     5041 attgtcgggc caaatggtgc aggaaaatcg acgttattaa gcctggttac tggcgatcat
     5101 ccgcaaggtt acagcaacga tttgacgctt ttcggacgac gtcgcggcag cggcgaaacc
     5161 atctgggata tcaaaaagca tatcggttac gtcagcagta gtttgcatct ggattaccgg
     5221 gtcagcacta ccgtgcgtaa tgtgattctt tctggctatt ttgattcgat tggcatttat
     5281 caggccgttt cggatcgcca gcaaaaactg gtgcagcagt ggctggatat tctcggcatt
     5341 gataaacgta cggctgacgc tccgttccat agtctttcct ggggacagca gcgtctggcg
     5401 ctgatcgtcc gcgcactggt gaaacatccg acgttgctta ttctcgatga accactacag
     5461 gggcttgatc cgctcaatcg ccagcttatc cgccgttttg ttgatgtgct gattagcgaa
     5521 ggtgaaacgc aattgttatt tgtttcgcac cacgctgaag atgcgcctgc ctgtattacc
     5581 catcgccttg agttcgtgcc ggacggtgga ctctatcgct atgtgctgac aaaaatatat
     5641 tgagtcggta gtgctgacct tgccggaggc ggcctaagcg ccctctccgg ccaacggttc
     5701 gacgcatgca ggcatgaaac cgcttctttt ttcagataaa aagcgcaatc attcataaac
     5761 cctctgtttt ataatcactt aatcgcgcat aaaaaacggc taaattcttg tgtaaacgat
     5821 tccactaatt tattccatgt cacacttttc gcatctttgt tatgctatgg ttatttcata
     5881 ccataagcct aatggagcga attatgagag ttctggttac cggtggtagc ggttacattg
     5941 gaagtcatac ctgcgtgcaa ttactgcaaa acggtcatga tgtcatcatt cttgataacc
     6001 tctgtaacag taagcgcagc gtactgcctg ttatcgagcg cttaggcggc aaacatccaa
     6061 cgtttgttga aggcgatatt cgtaacgaag cgttgatgac cgagatactg cacgatcacg
     6121 ctatcgacac cgtgatccac ttcgccgggc tgaaagccgt cggcgaatcg gtacaaaaac
     6181 cgctggaata ttacgacaac aatgtcaacg gcactctgcg cctgattagc gccatgcgcg
     6241 ccgctaacgt caaaaacttt atttttagct cctccgccac cgtttatggc gatcagccca
     6301 aaattccata cgttgaaagc ttcccgaccg gcacaccgca aagcccttat ggcaaaagca
     6361 aattgatggt ggaacagatc ctcaccgacc tgcaaaaagc ccagccggac tggagcattg
     6421 ccctgctgcg ctacttcaac ccggttggcg cgcatccgtc gggcgatatg ggcgaagatc
     6481 cgcaaggcat tccgaataac ctgatgccat acatcgccca ggttgctgta ggccgtcgcg
     6541 actcgctggc gatttttggt aacgattatc cgaccgaaga tggtactggc gtacgcgatt
     6601 acatccacgt aatggatctg gcggacggtc acgtcgtggc gatggaaaaa ctggcgaaca
     6661 agccaggcgt acacatctac aacctcggtg ctggcgtagg cagcagcgtg ctggacgtgg
     6721 ttaatgcctt cagcaaagcc tgcggcaaac cggttaacta tcattttgca ccgcgtcgcg
     6781 aaggcgacct tccggcctac tgggcggacg ccagcaaagc cgaccgtgaa ctgaactggc
     6841 gcgtaacgcg cacactcgat gaaatggcgc aggacacctg gcactggcag tcacgccatc
     6901 cacagggata tcccgattaa ggaacgacca tgacgcaatt taatcccgtt gatcatccac
     6961 atcgccgcta caacccgctc accgggcaat ggattctggt ttcaccgcac cgcgctaagc
     7021 gcccctggca gggggcgcag gaaacgccag ccaaacaggt gttacctgcg cacgatccag
     7081 attgcttcct ctgcgcaggt aatgtgcggg tgacaggcga taaaaacccc gattacaccg
     7141 ggacttacgt tttcactaat gactttgcgg ccttgatgtc tgacacgcca gatgcgccag
     7201 aaagcaacga tcctttgatg cgttgccaga gcgcgcgcgg taccagccgg gtgatctgct
     7261 tttcaccgga tcacagtaaa acgttgccag aactgagcgt tgcggcattg acggaaatcg
     7321 tcaaaacctg gcaggagcaa accgcagagc tgggaaaaac gtacccgtgg gtgcaggtct
     7381 ttgaaaacaa aggtgcggcg atgggctgct ctaacccgca tccgcacgga cagatttggg
     7441 caaatagctt cctgcctaac gaagctgagc gcgaagaccg cctgcaaaaa gaatattttg
     7501 ccgaacagaa atcaccaatg ctggtggatt atgttcagcg cgagctggca gacggtagcc
     7561 gtaccgttgt cgaaaccgaa cactggttag ccgttgtgcc ttactgggct gcctggccgt
     7621 tcgaaacgct actgctgccc aaagcccacg ttttgcggat caccgatttg accgacgccc
     7681 agcgcagcga tttggcgctg gcgttgaaaa agctgaccag tcgttatgac aacctcttcc
     7741 agtgctcctt cccctactct atgggctggc acggcgcacc atttaatggc gaagagaatc
     7801 aacactggca gctgcacgcg cacttttatc cgcctctgct gcgctccgcc accgtacgta
     7861 aatttatggt tggttatgaa atgctggcag agacccagcg agacctgacc gcagaacagg
     7921 cagcagagcg tttgcgcgca gtcagcgata tccattttcg cgaatccgga gtgtaagaaa
     7981 tgagtctgaa agaaaaaaca caatctctgt ttgccaacgc atttggctac cctgccactc
     8041 acaccattca ggcgcctggc cgcgtgaatt tgattggtga acacaccgac tacaacgacg
     8101 gtttcgttct gccctgcgcg attgattatc aaaccgtgat cagctgtgca ccacgcgatg
     8161 accgtaaagt tcgcgtgatg gcagccgatt atgaaaatca gctcgacgag ttttccctca
     8221 atgcgcccat tgtcgcgcat gaaaactatc aatgggcgaa ctacgttcgt ggcgtggtga
     8281 aacatctgca actgcgtaac aacagcttcg gcggtgtgga catggtgatc agcggcaatg
     8341 tgccgcaggg tgccgggtta agttcttccg cttcactgga agtcgcggtc ggaaccgtat
     8401 tgcagcagct ttatcatctg ccgctggacg gcgcacaaat cgcgcttaac ggtcaggaag
     8461 cagaaaacca gtttgttggc tgtaactgcg ggatcatgga tcagctaatt tccgcactcg
     8521 gcaagaaaga tcatgccttg ctgattgact gccgctcact ggggactaaa gcagtttcca
     8581 tgccgaaagg tgtggctgtc gtcatcatca acagtaactt caaacgtacc ctggttggca
     8641 gcgaatacaa cacccgtcgt gaacagtgcg aaaccggtgc gcgtttcttc cagcagccag
     8701 ctctgcgcga tgtcaccatt gaagagttca acgctgttgc acatgagctg gacccaatcg
     8761 tggcgaaacg cgtgcgtcat atcctgactg aaaacgcccg caccgttgaa gctgccagcg
     8821 cgctggagca aggcgatctg aaacgtatgg gcgagttgat ggcggagtct catgcctcta
     8881 tgcgcgatga tttcgaaatc accgtgccgc aaattgacac tctggtagaa atcgtcaaag
     8941 cagtgattgg cgacaaaggt ggcgtacgca tgaccggcgg cggatttggc ggctgtatcg
     9001 tcgcgctgat cccggaagag ctggtgcctg ccgtacagca agctgtcgct gaacaatatg
     9061 aagcaaaaac aggtattaaa gagacttttt acgtttgtaa accatcacaa ggagcaggac
     9121 agtgctgaac gaaactcccg cactggcacc cgatggtcag ccgtaccgac tgttaacttt
     9181 gcgtaacaac gcagggatgg tagtcacgct gatggactgg ggtgcgactt tactttcagc
     9241 tcgtattccg ctttccgatg gcagcgtccg cgaggcgctg ctcggctgtg ccagcccgga
     9301 atgctatcag gatcaggccg cgtttctggg ggcctctatt ggtcgttatg ccaaccgcat
     9361 cgccaatagc cgttatacct ttgacggtga aacggtgacg ctttcgccaa gtcagggcgt
     9421 taaccagctg cacggcggcc cggaagggtt cgacaaacgt cgctggcaga ttgtgaacca
     9481 gaacgatcgt caggtgctgt ttgccctgag ttcagatgat ggcgatcagg gcttcccggg
     9541 taatctcggc gcgacggtgc aatatcgtct gaccgacgat aaccgtatct ccattactta
     9601 tcgcgccaca gttgataaat cttgcccggt gaatatgact aatcacgtct atttcaatct
     9661 tgacggcgag cagtctgacg tgcgcaatca caagttgcag attctggcgg gcgaatatct
     9721 gccggttgat gaaggcggca ttccgcacga cggcctgaaa tctgtcgccg gaacctcttt
     9781 tgatttccgc agcgccaaaa tcatcgccag tgagtttctt gccgacgacg atcagcgcaa
     9841 agtgaaaggt tacgatcacg cattcttgtt acaggccaaa ggcgatggca agaaagtggc
     9901 ggcgcatgtc tggtcagcag atgaaaaatt gcagctgaag gtctacacca ccgctcccgc
     9961 tcttcaattc tactccggca acttcctcgg cggcacaccg tcgcggggaa ccgaacctta
    10021 cgccgactgg caaggactgg cgttggaaag cgagtttctg ccggacagcc cgaaccaccc
    10081 tgaatggccg caaccggact gcttcctgcg tcctggcgaa gagtattcca gcctgacgga
    10141 atatcagttt attgctgagt aatgttaagc cctcgtcatg agggctttat cttcatattg
    10201 ttcaaatcac cagcaaacac cgacatattt gcaactcaat attcacaaca accttacact
    10261 gcgccactat tttcgctatg gttatgcgta agcattgctg ttgcttcgtc acggcaatat
    10321 aatgagaatt attatcataa aaagataatt tgaggagtaa gtatatggct gtaactaagc
    10381 tggttctggt tcgtcatggc gaaagtcagt ggaacaaaga aaaccgtttc accggttggt
    10441 acgacgtgga tctgtctgag aaaggcgtaa gcgaagcaaa agcagcaggt aagctgctga
    10501 aagaggaagg ttacagcttt gactttgctt acacttctgt gctgaaacgc gctatccata
    10561 ccctgtggaa tgtgctggac gaactggatc aggcatggct gcccgttgag aaatcctgga
    10621 aactgaacga acgtcactac ggtgcgttgc agggtctgaa caaagcggaa accgccgaaa
    10681 agtatggcga cgagcaggtg aaacaatggc gtcgtggttt tgccgtgact ccgccggaac
    10741 tgactaaaga tgatgagcgt tatccgggtc acgatccgcg ttacgcgaaa ctgagcgaga
    10801 aagagctgcc gctgactgaa agcctggcgc tgaccattga ccgcgtgatc ccttactgga
    10861 atgaaactat tctaccgcgt atgaagagcg gtgagcgcgt gatcatcgct gcacacggta
    10921 actctttacg tgcgctggtg aaatatcttg ataacatgag tgaagaagag attcttgagc
    10981 tgaatatccc gaccggcgtg ccgctggtgt atgagttcga cgagaatttc aaaccgctga
    11041 aacgctatta tctgggtaat gctgacgaga tcgcagcgaa agcagcggcg gttgctaacc
    11101 agggtaaagc gaagtaaacg tcattcgttt aaaaatgaga aagccgactg caagtgagtc
    11161 ggcttttttg ttgctaacaa tggagcacat tgcctgatgc gacgctgcgc gtcttatcag
    11221 gcctgtggtg attcatcgga tacgccactc tgacggcgca tccgacaatt aaaccttacc
    11281 cgcgacgcgc ttttactgca ttcgccagtt gacgtaacag agcatcggta tcttcccagc
    11341 caatgcaggc atcggtgatg ctcttaccgt aggccagcgg ctccccgcta tcgaggctct
    11401 gattgccttc caccagatgg ctttccacca tcacgccaat aatggccttt tcgccaccgg
    11461 caatctgctg gcaaacgtca gcacaaacat ccatctgctt tttgaattgt ttggacgagt
    11521 tagcatggct gaaatcgatc atcacctgtg ctggcaggcc tgctttgttc agcccttctt
    11581 tcacttcagc aacgtgcttc gcgctgtagt taggctcttt accgccgcgc agaatgatat
    11641 ggcaatcacc gttaccgctg gtattcacaa tcgccgaatg cccccattta gttacggaca
    11701 ggaagcagtg cggcgcaccg gcggcattaa tggcatcgat agccacttta atcgtaccgt
    11761 ctgtgccatt tttaaaacct accggacaag aaagaccaga cgccagttcg cggtgcacct
    11821 gcgattccgt ggtacgtgca ccaattgcgc cccagctcat caggtcagcg agatactgag
    11881 gagtgatcat atcgagaaac tcacccgccg ctggcagacc gctgtcgtta atatcaagca
    11941 gcaatttacg ggctatacgc agaccgtcgt tgatctggaa gctgttatcc atatgcggat
    12001 cgttaatcaa ccctttccag cccaccgtgg tacgcggctt ttcaaaatag acgcgcatta
    12061 cgatttccag ctcatctttc agctcttcac gcagcgccag caagcgagtg gcatactctt
    12121 tagccgcgac aggatcatga attgagcacg ggccaatcac caccaacagg cgatcatcat
    12181 tacctttcag gatcttatgg atcgcttttc gggcatgggc aaccgtattc gcggcatttt
    12241 cagttacggg gaatttttcc agcaatgcga caggaggaag taactctttg atttctttga
    12301 tgcgtaaatc gtcgttctga taattcatgt ctgttccagt gttgccatac ttatcttagt
    12361 gaatgcaata cttccaatct atatcttccg tcagaatgtg taaacggggt tttacactat
    12421 gaacggatta atcctggaat tagcaagaaa aacgccagat tgtcgcgaaa aacgagatct
    12481 ctcctacaat ttctaactgt aactcctttg tttgttaatt atttcaagat tctctgctgc
    12541 gtttcataaa ctggctgaaa tcttaaacca atgccttata ttcacctgca aatgcactgt
    12601 tggaagaggt tatccgacat aacgaccata acaggagcat cctatgaaaa tgacaaaact
    12661 ggccacatta tttctgactg ccactctaag ccttgccagc ggtgccgcac tggctgccga
    12721 tagcggagcg caaactaata acggccaggc aaacgccgca gctgatgcgg gccaggtagc
    12781 ccctgacgcc cgtgaaaatg tcgcgccaaa taacgtcgac aataacgggg taaataccgg
    12841 ttctggcggc acaatgctgc attcggatgg ttcttcaatg aacaatgacg gaatgaccaa
    12901 agatgaagag cacaagaaca ccatgtgcaa agatggtcga tgtccggata tcaataaaaa
    12961 ggtacaaatc ggtgatggca tcaataatga tgtcgatacc aaaaccgacg gtaccacgca
    13021 gtaaccctgc aaaatagcca ataatgggag agcggagtct ctccctttta atttttcact
    13081 tctaaaagca gtatgatgtc ataaagttaa gtgagaataa ttaagggatg actcgtcatg
    13141 gcgcactcac actcacacac gtcctcacac ctgccagaag ataataatgc tcgtcgcttg
    13201 ttgtatgctt tcggcgtgac tgccgggttt atgctggtag aagtcgttgg tggttttctt
    13261 tctggttctc tggcattgct ggccgatgcg ggtcatatgt tgaccgatac tgccgccctg
    13321 cttttcgccc tactcgccgt gcaattttca cgtcgtcctc ccaccattcg ccacactttc
    13381 ggttggctaa gactgaccac gctcgcggcg tttgtgaatg ccatcgccct cgtggtaatt
    13441 accattttga ttgtctggga ggcgatagaa cgcttccgca cgccgcgtcc ggtcgagggc
    13501 ggcatgatga tggcaattgc cgtggctggg ttgctggcaa atatactttc tttctggtta
    13561 cttcatcacg gcagcgaaga gaaaaacctc aacgtacgag cagcggcatt gcatgtgctc
    13621 ggggacctgt tgggttcggt tggggctatt attgccgcgt tgattattat ctggaccggc
    13681 tggacacctg ctgaccccat tctctcgata gtggtgtcgc ttctggttct gcgtagcgcg
    13741 tggcgactgt tgaaagatag cgtgaatgaa ttacttgaag gtgcaccggt atcgctggat
    13801 atcgctgaac tgaagcgtcg tatgtgccgg gaaatcccgg aagtccgcaa tgtgcaccat
    13861 gtacatgtat ggatggtagg cgagaagccg gtgataacgc tgcatgtgca ggtgatcccg
    13921 ccacacgatc acgatgcctt gttggatcag atccaacact acctgatgga tcactatcag
    13981 attgagcacg ccaccattca gatggaatat caaccttgtc atgggccaga ctgccacctg
    14041 aatgaagggg tgtctggcca ttcacatcat caccattaat gggacagcgc gcgtgagcct
    14101 ctttcacgtg cgctgttgat ccacatccga ctgccgttga gcgcaataaa ggtcaggatg
    14161 atgtactcca gagacatggc gtaaacgccc tgaagtgcaa aaataacgac gctaatcacg
    14221 ttaataatca cccacaacag ccagttttcc acatacttac gcgtcatcag aatcattgcc
    14281 acgatagata acaccatcat gcatgaatcc cagaacggga aagcgtccgg ttgcagttca
    14341 ggcatcacaa cctgtaatcc taatgcttgc atgatcatga ctgccacgcg ggtcaaaaat
    14401 gcaaacaccg gattgataaa taccgtcatc agaccaatcg aaacaacgca aaccgccaac
    14461 cagctgagcg ccttcggcaa tggtaaccag cgaattttca actccgcctc gttctgactg
    14521 gtttgtcgcg accacgcata ccaaccgtaa atattcgcgg caaagaaaaa cacctgtaat
    14581 agcaggctgg catacagctg aatctgaaag aaaataatgc caaacaaggt gacgttgatc
    14641 aggccaaaga agtagttgct gatcttctcc agactggcaa ggccaataca cagcaacccg
    14701 gcgatcgtgc ctaccgcttc gatccatgag agatcataac cgcctgcccc tattggtata
    14761 tgtaccagga tattctgcac actaaaaaaa tccatctttc ccccaaagcg taacgttacg
    14821 ccttattatt cgttatccac gtagtgtagc cgcaaaatcc agcatacgat tgagcggcac
    14881 cagcgccctc tctcgcagcc tttcatcaag aacctcgtga ttgcttcctt cctgttctaa
    14941 tgcctctgcg atggactgaa ggccattcat ggccatccac ggacaatgcg cgcag
//
LOCUS       DAJVQG010000100        14931 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJVQG010000100 DAJVQG010000000
VERSION     DAJVQG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14931
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.100"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            313..1611
                     /gene="kgtP"
                     /locus_tag="OQM83_002752"
     CDS             313..1611
                     /gene="kgtP"
                     /locus_tag="OQM83_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCS1520972.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1608..1931)
                     /locus_tag="OQM83_002753"
     CDS             complement(1608..1931)
                     /locus_tag="OQM83_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCS1520973.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="OQM83_002754"
     CDS             complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="OQM83_002754"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCS1520974.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3446..6106)
                     /gene="pat"
                     /locus_tag="OQM83_002755"
     CDS             complement(3446..6106)
                     /gene="pat"
                     /locus_tag="OQM83_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCS1520975.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="OQM83_002756"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="OQM83_002756"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCS1520976.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="OQM83_002757"
     CDS             complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="OQM83_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCS1520977.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7531..8568
                     /locus_tag="OQM83_002758"
     CDS             7531..8568
                     /locus_tag="OQM83_002758"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCS1520978.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8616..9305)
                     /gene="ung"
                     /locus_tag="OQM83_002759"
     CDS             complement(8616..9305)
                     /gene="ung"
                     /locus_tag="OQM83_002759"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCS1520979.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9610..9993
                     /gene="grcA"
                     /locus_tag="OQM83_002760"
     CDS             9610..9993
                     /gene="grcA"
                     /locus_tag="OQM83_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCS1520980.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="OQM83_002761"
     CDS             complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="OQM83_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCS1520981.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10739..11620
                     /locus_tag="OQM83_002762"
     CDS             10739..11620
                     /locus_tag="OQM83_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1520982.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="OQM83_002763"
     CDS             complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="OQM83_002763"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCS1520983.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13295..14032
                     /gene="trmN"
                     /locus_tag="OQM83_002764"
     CDS             13295..14032
                     /gene="trmN"
                     /locus_tag="OQM83_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCS1520984.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14017..>14931)
                     /locus_tag="OQM83_002765"
     CDS             complement(14017..>14931)
                     /locus_tag="OQM83_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCS1520985.1"
                     /translation="NLEFNQFPPTALYHPQARNFLLTEALRGEGAYLKRPDGTRFMPD
                     FDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLGLGID
                     LTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSLLECL
                     VYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDYVGIV
                     RTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKESRG
                     LHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3603 a         3776 c         3910 g         3642 t
ORIGIN      
        1 aaaggccatc ctgacggatg gcctttttgc attggcgcag aaaaaaatgc ctgatgcgac
       61 actgcgcgtc ttatactccc acatatgcca gattcagcaa cggatacggt ttccccaact
      121 tgcccacttc catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataacca
      181 ttttcctgct aactaaattc atggttaagg ttgcacaatg atatgcaaca aatgtataac
      241 attcccttta ccaaaaaaaa taaacaaaag cgaccgacaa aagcatcgga ttacggcagg
      301 agacataatg gcatggctga aagtactgta acggcagaca gcaaactgac aagtagtgat
      361 actcgtcgcc gcatttgggc aattgtgggg gcctcttcag gtaatctggt cgagtggttc
      421 gatttctatg tctactcgtt ctgttcactc tacttcgccc acatcttctt cccttccggg
      481 aacacgacga ctcaactact acaaacagca ggtgtttttg ctgcgggatt cctgatgcgc
      541 ccaataggcg gttggctatt tggccgcata gccgataaac atggtcgcaa aaaatcgatg
      601 ctgctatcgg tgtgtatgat gtgtttcggc tcactggtaa tcgcctgttt gcctggttat
      661 gaaacaatcg gtacgtgggc tccggcatta ttgcttctcg cgcgtttatt tcagggatta
      721 tccgttggcg gagaatatgg caccagcgcc acctatatga gtgaagttgc cgttgaaggg
      781 cgcaaaggtt tttacgcatc atttcagtat gtgacgttga tcggcggaca actgctagcc
      841 ctactggttg tcgtggtttt acaacacacc atggaagacg ctgcactcag agagtgggga
      901 tggcgtattc ctttcgcgtt aggagctgtg ttagctgttg tggcgttgtg gttacgtcgt
      961 cagttagatg aaacttcgca acaagaaacg cgcgctttaa aagaagctgg atctctgaaa
     1021 ggattatggc gcaatcgccg tgcattcatc atggttctcg gttttaccgc tgcgggctcc
     1081 ctttgtttct ataccttcac tacttatatg cagaagtatc tggtaaatac tgcgggaatg
     1141 catgtcaacg tggcgagtgg cattatgact gccgcattgt ttgtattcat gcttattcaa
     1201 ccactcattg gcgcgctgtc ggataagatt ggtcgccgta cctcaatgtt atgtttcggt
     1261 tcgctggcag ccatttttac cgttcctatt ctctcagcat tgcaaaacgt ttcctcgcct
     1321 tatgccgctt ttggtctggt gatgtgtgcc ctgctgatag tgagttttta tacatcaatc
     1381 agtggaatac tgaaggctga gatgttcccg gcacaggttc gcgcattagg cgttggcctg
     1441 tcatatgcgg tcgctaatgc tatatttggt ggttcggcgg agtacgtagc gttgtcgctg
     1501 aaatcaatag gaatggaaac agccttcttc tggtatgtga ccttgatggc cgtggtggcg
     1561 tttctggttt ctttgatgct acatcgcaaa gggaagggga tgcgtcttta gtgacgggtc
     1621 agttgccaga cggtatagcc ggtgcttgca ccggcgacat cccaggccaa atccttccag
     1681 ctccagccgc tcccttcggg gcggctatcc caaagctctt ttgacgcccc caaactgaca
     1741 gagaacatca atccaaacat ggcactgcga tcccggctca tcccctgatg ctgtgaatat
     1801 tcatttccgg cggcggaaag catcgccgag gcgataaagt gttgagcttt atcctgcccg
     1861 ctccagctat cgttagccat atggctgcat ccagaaagta acaatagcga acagacaaaa
     1921 agaatacgca aaacgtggcc tccaaaaaac aaaccccgta caggacgggg ttgtgattac
     1981 aggatgcggc taattaatcg gtcgatgcgg atacggcgca aacggcggat gagtttacga
     2041 accttcaccg gataatcggc aatactttgc agatcgcgat agtgcttaac gatggtggta
     2101 tgctcgcgga tcagctccag ttctttctct cgctgcggcg caagcttcag ttgtggatcg
     2161 tggatcaaaa tggcgttttc cagatccaga cgccaggcgc gcgggttcag gttattaccg
     2221 gtgatcaaca tccacttatc atcaacccac atccctttca ggtgataggt gttgtcgtca
     2281 tctttccata accgaaccac tagctggtca gtattgacgt aatactgcaa acggctcagg
     2341 aaacgacgca gattgatctc atagagataa ggcaatgcgc caattatctt gaaaggttca
     2401 tcttccggaa tgtagaagtc gttcgcggtt ttatcaccaa caataatttc gacctttttc
     2461 ccttcgcgca gcaactggat aatattgcgc acaaggattg ctggcaggtt gaagtatggc
     2521 gtacagatgg ttagtttctg ctcggcacaa ggcataagat ggaaaatggt cttgttcaac
     2581 agactcgatt tccccagccc cactagcggc gttacggaaa gctgatcgtt gtcggcatcg
     2641 ccctggaaat gataagcggc atcacgcagc tcctggcgga acagacgaat atcgttcttg
     2701 atttccgggc tttttggccg attaacatca tccagacgat taacgccgcg gccattcata
     2761 atattctgtg taacccattc aaacataatg tctgacatct tacggttacg gataagatga
     2821 taacggtcgt agcgatattt atcgtgctga tgcaggtaaa catcgttcag gctggcaccg
     2881 ctataaagta cgctatcgtc gatgataaag cctttaaagt gcagaacacc aagggcttca
     2941 cgggtattga ttggaacgcc ataaaccgga acatctacgc ccgaattttc ctgcgccatg
     3001 cggcagtacc agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc acgttgtgca
     3061 cgatgccagt cgaccagcac ccgcacatcc agttccggac gctgcctttt agcttcatac
     3121 aacgcgttca gaatgccttt gccaccgtca tcctgttcga gatacagggc gacaatgcaa
     3181 atgcgctgct tcgcgctggc tattttttcc agcagcgtct cccggaagtc ggcgggagcg
     3241 taaaagaaat cgacatcatc aactgattga gaaatcttgg gtagttgggc aaggtgttgt
     3301 tgatgtttat tacgcttaaa ttttgacaac atcacagtgc atttcttctc tgttcattga
     3361 agggtcctct gtgcaatgca gacgacataa gcgggcaata ataacaccag tcccgattaa
     3421 gtggtcaaca tttccagtac cttactcatg attcctcgcg ttgggcaaga tttagcgtaa
     3481 gcccaacgat cccctcttcg agctggatat caacgttaaa cccgagcttg cgggctagcg
     3541 ccaccatgcc acgattgttt ggcatcgtaa taccattcag acgttgtagt ccgtgatctc
     3601 gcgtataggt aatcaacttt tccattaagc gtcgacctaa gcctaaccct ttgagatccg
     3661 agcgaaccag cacagcaaat tcggcatcga tgttatcagg atcggaaatc gcacgcgtga
     3721 cgccgaggat ctcttccgtt tgatcaatac gtcgtaccgc tacaaacgcc atttcccgat
     3781 cgtagtcgat ctgtgtcatg ttggctaaat cttcatgggt aaattcgttg atctcgctaa
     3841 agtagcggta ataaagatct tctttggtga ctcgcgaaat gaattgctga agttgtggct
     3901 catcttctgg caaaatcggg cggaacaagc agcgttcacc gtttttcaat tctacccatt
     3961 cttccagctg atgcggataa gggcgcactg ccagccgact ctcgttatcg ccttcaaacg
     4021 gcgagatatc cagcgtgaca tccagcgcgg taaattcact gccagaagcc agcaaaggat
     4081 gaatatccag acgctgaatt tccgggcaat cgacaatcaa gttggaaacc tgcaccagaa
     4141 gctggctcaa gcctgcaaca tccaatgggc gtagcgcact gcgcgcacga atctttttac
     4201 ttttgatccc ctgaataacc agatagcggg ccaggttcat gttcagcggc ggcagtgcga
     4261 cgacggcttg atcttcagga cgccactcca caccgccttc acccagcatg atcaacggcc
     4321 cgaaaaccgg atcgtgctca accacaaccc gcaactcctg agcgccagca cggttagcca
     4381 tactttgcac caacaggccg tggacccgcg cctgtggcca ggccattttt acgcgatcga
     4441 aaatagcgtt cgccgcttgc tggacttcat tggctgtacg caggtaaagc atgacgccct
     4501 gaacttccga tttatgtgga atatccggcg aacgcaattt cagcgccacc ggataaccaa
     4561 tctgttcggc aatatgcacc gcttcggtgc tatcgctggc aatccaggta gggagcgtgt
     4621 tcatgccata cgcttgcagg atgggctgaa cttcatgggt atcgagcgac gtagcccctt
     4681 cggcaatcgc ctgttgcaac agaagatgcg cttctgcggt attggaagtc agattgctgg
     4741 gcaacgtcgg cgtttcgcgt agttgcttct gattacgccg gtactccacc atatgcataa
     4801 aagcagtgat ggttccttcc ggggtacggt aggtcggcag cccggcttcg ctgaataaac
     4861 gtcgtgcctc ttgcgaggag tgctcgccgc accagttcgt cagcaaagag acatatttgc
     4921 tgcggggatg atgctttacc gcttcaatta atacttgcgc gctttctgtt gcgggagcag
     4981 cggcgctggg cgaatgaata accatcagcg catcaaaatc ctggctgtgg agcagaatgt
     5041 ccagcgtttt gacataatgc tcgctgctgg cgtcatcgcg cagatcgaga gggttagaga
     5101 ctgccacatg ttctggcaat gcatcgcgca atttctggca ggtttcttcg cttagcgttg
     5161 ccagcttgcc attgcgtgac cataaggcat ccagcgccag cgcggcaggc gcagcaccgt
     5221 tgctgataat catcaaccgg tcgccacgca gcgggcgcat atggctaagg gtttccaccg
     5281 ccgaaaacag ttcgtgggta tcctgtaccc gcaacaaacc ggcacgctga atagccgcat
     5341 cccatgccgg gtccattcct gccgtcgtgt tgagcagtcg ctgtgccgcc gggctacgtc
     5401 cgcttttaat caccagaatc ggtttattac gcgaggcact acgggctgcc gaaacaaagc
     5461 gtcgcgcgtc gcttaactgt tcgagataga gcaggatggc gctggtttta ctgtcgcgcg
     5521 ccagatagtc gagcaattca tcaacgtcga tatccaggct gtcgccgagc gcaataaagt
     5581 aggaaaagcc catctcacgc tgttgcgccc agtcgaggat ggtgttggag acggctgccg
     5641 attgtgaaat aaacgccagc ttgccgcgtt taatcggcac aggcgaaaag ctggcattca
     5701 gaccttgcca gggagccagt aaacccagac tgtttggtcc aagcaggcgc atgttatggc
     5761 gcagggcgca ggcgcggaga tcttcgtgtt gcgatgccgg ggcggaaaga ataatgcagg
     5821 ttttacagcc tttctcgccg agctcttcca gaagagcaag attacggctg gcattggtac
     5881 ataaaaccgc aaggtcgggt gtaaagggca agctggcaat atccggccag gccaacacac
     5941 ccaacaccgc tttccaggct ggcgtcaccg ggagtaccgg tccgttaaag cttcccgcca
     6001 gcaggttacg catcatcagg taacctgcgc gattgggttt catcgacgcg ccaattaccg
     6061 ctatcgattt tggtcgcagt agtgcttcca gtcctcgctg actcataccg gtctcccgca
     6121 gaagtgaccg aatgatttta aacgctttct aactgttctg ctgtgatgct acccagatgt
     6181 tgcgtttttc ctgccagata gcgtgtttta aagcgggtaa aatgctcgcc taaccctgct
     6241 gccgccccgg tatcgccggc catatctaac agtgcgatgg ctacctcggc agtacaatat
     6301 tggcctgcag cctgggcttc acgcaggcga taggcagaaa gccgggaaag atcgacggaa
     6361 atgacgggaa gattatccag atacggactt ttacgaaaca tcttgcgagc ttccggccag
     6421 gtaccatcga gcatgataaa cagcggtggc ttaccggcag gtggtgtgaa gatcacttcc
     6481 cgttgctcat cagcatacga ggcgggaaag accaccattg gctgataatc cgggttttgt
     6541 accagatcca gcaaatcctg cgagggttcg gtacgcgacc attgaaacgc aacggtatca
     6601 ggcaaaatat cagcaatcag acgcccggta ttactgggct tcattggctc ggtgtcgaac
     6661 atcagcaaac agaagcgact ttttgcttgt gctggggtaa ttgtcgaaca gagacataat
     6721 ttctctggca aaagacagcg ttggcagcga tgaacgcgat taccgcgggc aagaaaagga
     6781 cgtgttgcgc gcgcaatacg ctcggcgcgt aactggagaa cagcgttttc ggtcataaga
     6841 gagcgtcgaa aaaacgccat tgtcgcagag gagaaaacgg ggcacaagat gcaccccggt
     6901 aagattaaag agattcgttc agccagctat cgaacggcgc tttcggtact gcgccattaa
     6961 gcatgtcgac aacctgaccg tttttgaaaa tcatgatcgt cggtatacta cgaattccaa
     7021 agcgactgct caattcacgt tcagcttcgg tattcacttt cacaaagcgc actttaccgc
     7081 tacgctcttg cgcgacatct tcaaaaattg gtgcgaaatt acggcagggg ccgcaccacg
     7141 gtgcccagaa gtcgatcacc acaggtagat catccttcag caatttgtcg agtgtttcac
     7201 cggtcgcatt aatcacctct ccgtcaaaca agtcgtgacc gcagcgtccg cattttgccg
     7261 catcttcgat ccgatcgtcg ggaatgcgat tgatggcctg acaatgggta caaacggtat
     7321 tcataactaa cctcgggatg agtaaggaga cttatcggca acgacgccgg tatgtttcta
     7381 atatgttaca tattatcggg gacattgttc cagacaacaa tctgttttat tcaataggta
     7441 tgatagtcgc aggctttggt acgaaatcat ggcgaaccgc gcggacttcg ggtaatctgc
     7501 gcgcttcgcg cagcgctggt ggagaaaagc atgaacgatg aaatgaaagg taaaagcggc
     7561 aaggtcaaag tgatgtatgt ccgcagtgat gatgattctg ataaacgtac ccacaacccg
     7621 cgtaccggga aagggggcgg gcgtccagga aaatctcgtg ctgacggtgg ccgtcgcccc
     7681 gcccgcgatg acaaacagag tcagccccgt gaccgcaagt gggaagattc gccgtggcgc
     7741 acggtttccc gcgcgccggg tgatgagacg ccggaaaagg ccgatcacgg tggcatcagt
     7801 ggtaaaagtt ttattgatcc ggaagtgttg cgtcgtcagc gtgcggaaga aacccgcgtc
     7861 tacggcgaaa atgcctgtca ggcactgttc cagagccgtc cggaagcaat tgttcgcgcc
     7921 tggtttatcc agagcgtaac gccacgtttt aaagaagcct tgcgctggat ggcggcaaac
     7981 cgcaaagcgt accatgtggt ggatgaagct gaattgacaa aagcgtcagg cacggaacat
     8041 cacggcggcg tttgcttcct gatcaaaaag cgtaacggta caaccgtgca gcagtgggta
     8101 agccaggcag gcgcgcagga ttgcgttctg gcactggaaa atgaatctaa cccgcataac
     8161 ctgggtggca tgatgcgcag ctgcgcgcac ttcggcgtga aaggtgtcgt ggtgcaggat
     8221 gcggcactgc tggagtcggg ggcggctatc cgtaccgcag aaggcggcgc agagcacgtt
     8281 cagccgatta ctggcgacaa cattgttaac gtgctggatg atttccgtca ggcaggttac
     8341 accgtagtga ccacttccag cgagcagggt aaaccgctgt tcaaaactag tctgccagcg
     8401 aaaatggtac tggtgctggg tcaggaatat gaagggttac cggatgccgc acgcgatccg
     8461 aacgatctgc gcgtgaagat tgacggtacg ggcaacgttg ccgggctgaa tatctctgtc
     8521 gcaaccggcg ttttgcttgg tgaatggtgg cgtcagaata aagcctgata aatcagccgg
     8581 gtggcaactc tgccatccgg catttccccg caaatttact cactctctgc cggtaatact
     8641 ggcatccagt caatcggcgt ctcgccacgt tgttccagcc actgatttgc cagcacaaaa
     8701 tggttgcagc caaagaatcc acgatgcgcc gaaagcggcg acggatgcgg tgctttcagt
     8761 acatgatggc gttgcttatc tataatcgcc cctttctttt gcgcatgtga tccccacaac
     8821 aaaaacacca cgccttcgcg gtgctggtta atcaggctga tgactttatc agtaaacgtc
     8881 tcccaaccga ggctggcgtg ggaatgcgcc tgacctgcgc gaaccgtcaa cacagtattg
     8941 agcagcagaa cgccctgacg cgcccagctt tcgagatagc catgattagg gcgggtgaag
     9001 cccggaatag tattttccag ctctttatac atattcaata atgacggagg aatggcaatg
     9061 ccgggacgaa cggaaaatgc cagaccatgc gcctgtcccg gtccgtgata aggatcctgg
     9121 ccgagaatca ccactttaac gtcacccaac tcggtaaagc ggaacgcgtt aaagacatct
     9181 ttttgtggtg gatagatagt gacgccggac tgccgctcgc tggcgacggt ctgaagggta
     9241 ttaagaaaat aaggttgttg cttctcttca gccagcacgt catgccaggt taattcgtta
     9301 gccatctcgc tctcctgcga atcttcaatc cgcctagctt aactgcttct ttctacagaa
     9361 caaaatcatg ctcatttttt agacttaaac gagtgcaaaa aaatgtaaat tggtaaaatt
     9421 tcacacccgc ataagttgat gtaaaacaat aaaatccatt tacgaccaca atcgatgtgt
     9481 ggtttttatt gatttaaatc aaagaatcaa gggtggttga ggagtatata tacactcaag
     9541 caacaatggt tttaccaatt ggccgcgaca ggctgaacaa atcaaataat tttgccgggg
     9601 aggcatcaca tgattacagg tatccagatt actaaagccg ctaacgacga tctgctgaac
     9661 tctttctggc tgctggacag cgaaaaaggc gaagcacgtt gcatcgttgc aaaagcaggt
     9721 tatgcagaag atgaagtggt tgcagtaagc aaactgggtg acattgaata ccgtgaagtt
     9781 ccagtagaag tgaaaccaga agttcgcgtt gaaggtggtc aacacctgaa cgttaacgtt
     9841 ctgcgtcgtg aaactctgga agatgcagtt aagcatccgg aaaaatatcc gcagctgacc
     9901 atccgtgtat ccggttatgc agttcgcttt aactctctga ctccggaaca gcagcgcgac
     9961 gttatcgctc gtacctttac tgaaagcctg taaggcttaa gtagcaagtt actaagcgga
    10021 agagggcgac ctcttccgct ttttttcgtt aatagaaaat gcgtaccgcg caatagacca
    10081 gtaacagggc aagcacgata tttaactggc gaccatactg gcgaaacaat cgctgaaaca
    10141 gatgccccgc cagcgcccag cacacattgc caaacgtccc aatcatcgcc agcaaaacgc
    10201 tgacgccaac tacccagctt agcgcctgtg tttgcggcag aacaaacgtc gacagtgccg
    10261 taacaccgta caaaatgatt ttgacgttca caaactgcaa agcaaagctg gcccaaaagc
    10321 tgattggttt tgcctgaagt ccgtcttcct ttgttgggct ggtggcgatt ttccacgcca
    10381 gccagacaat atatgccgcc cccgcccaac tcaaaaggtg taccgctgcc gggtcaatca
    10441 ctgccagtga aaatgaaatg cccgcacaca gtaacatcac aatcaaaaat cccagactca
    10501 tccctgccag cacgcgggta ctttgacgaa atccatgcga cgtagcagag ctaagggcga
    10561 gaatattgtt cggtcctggc gtcatagcgg taatcagggt gtaagtccaa aaagcactta
    10621 aaagggtcgg tgtcacttgt tatctcctct gttttggtag acagtacccg acacggaaca
    10681 tttaaaaaaa tgaatgtttt tatattctag attcgatatt ttcgataggt ttggggttat
    10741 ggatctgcgc cgttttatta cgcttaaaac cgtggtggaa gagggttcct ttttgcgagc
    10801 ttcgcaaaaa ttgtgctgta cacaatcgac ggtgactttt catattcagc agcttgagca
    10861 ggagttttca gtccagttat ttgagaaaat tggccgacgg atgtgcctta cccgcgaagg
    10921 aaaaaagttg ctgccgcaca tttatgaact tacccgagtg atggatacgc ttcgtgaggc
    10981 tgccaaaaag gagtcagatc cggacgggga actacgcgtt gtttcgggcg aaacgttgct
    11041 ctcttaccga atgccgcagg tgttgcagcg ttttcggcaa cgcgcaccaa aagtgcgttt
    11101 atcgttgcag tcgctgaact gttacgtgat ccgtgatgcg ctactgaatg atgaggccga
    11161 tgtcggcgtc ttttatcgcg tagggaatga tgatgccctg aaccgacgag agttgggtga
    11221 acaatcgctg gtgctggtgg cttcaccaca aattgtagat gtcgatttta ctgagccggg
    11281 aagacataac gcctgtagct ttattatcaa tgaaccacaa tgtgtcttcc ggcagatatt
    11341 tgagagcacg ctgcgtcagc ggcggatcac ggtggaaaac accattgagc ttataagtat
    11401 cgaaagcatc aagcgttgtg tcgcggcgaa tatcggagtc agctacctgc cgcgttttgc
    11461 ggtggcaaaa gagttggagt gcggagaatt aattgaattg ccttttggcg aacagtcgca
    11521 gaccattacg gccatgtgcg cacaccatgc tggaaaagcg gttagcccgg cgatgcacac
    11581 ttttattcag tgtgttgaag agagttttgt ggctggataa aaataatgtc gggattaaac
    11641 ccggcattta acttgtcgac aaagtgcgct ttattcatgc cggatgcggc gtgaacgcct
    11701 tatccggcct acaagatcgt gcaaattcaa tatattgcat gagccacgta ggcctgataa
    11761 gcgtagcgca tcaggcagtt ttgcgtttgc cagcactttt aataccaggc ttaaacccgg
    11821 cattgagatt actcttctgt cgtttgcggt ggcacgcccg ttccgcttgg tttacggcgc
    11881 ttaccaatat ttttggtgtc gcgatggcgt tttttcaccc gcggtttctc tttctcttta
    11941 gcttttttct tctcggcgcg ttttgccagt actttcttcg atggcttgcc ggtctgcttc
    12001 tcgcttggcg cacgcgtttt cgggcgtaac tcatcaataa cgcgagcttt aatcggctct
    12061 tcaatgtaac ggccaacctt gcccagcagc aaatggtcat gggcttccac cagcgaaatt
    12121 gcggtgcctt tacgaccggc gcgcgcggta cgtccgatac ggtgcaaata agtatcgcca
    12181 ctgcgcggca tatcgaagtt aaagacgtgg ctgacgtcag gaatgtcgat accgcgcgcg
    12241 gcaacatcgg ttgcaaccag tacgtttacg cgaccttcgg tcaaacgctt gatcgcttcg
    12301 ttacgcttgc cttgtaccat ctcaccttcg agatagcagt tgttgatgcc cgcttcgcgc
    12361 agccagtttg ccagctcatg cacacgttca cgcttacgca caaacacaat tgagcgggtc
    12421 gcttccggct gttttaacag atgcaccagc aatgcggttt tatgttccag gtcatcggcg
    12481 cggtaatacc actgatgaat ttttttgcgc tcacgggtgg acggattggc agaaacctcc
    12541 accggatctt ccagcagacg ctcggcaaag tcctgaatcg catcgccttc cagcgttgcc
    12601 gaaaagagca gggtctgttt acgccagcgc gtttcgccag caatatgttc gatatcctga
    12661 gcgaagccca tatccagcat acggtctgct tcgtcgagga tcagcgtttc aaccgcgcgg
    12721 caatcgaagt tctcttcttt tatgtattgc agcagacgtc cggtcgtggc gaccacgatg
    12781 tcctgatttt cgctgaacac ttccgcgtgg ttcatatagg ctacgccgcc ggtgatggtg
    12841 gcgatatcca gatgcgtatg tttcgccagt tcgcgggcat gatcggccac ctgcatcgcc
    12901 agctcgcgag ttggggtgag gatcaaaata cgcggcggac cggatttctt acgtgggaaa
    12961 tcgagcaggt gctgcaacgc tggcagcaga tacgccgccg ttttaccggt gcctgtcggc
    13021 gcagaaccga gtacatcacg gccatcgagc gcaggcggaa tggcggcagc ctgaatggcg
    13081 gtcgggcgag tgaaaccttt atcctggagg gcttccagca ggctttcgtc gagttcaagt
    13141 tcggaaaaag tcgttacagt catgttctac ctctgtgtgg ggcgctgatt atagacgtta
    13201 cggctgcaat cttcatctgt ttgtatggat atcgcttctc gggtattgtt ctcacccggt
    13261 gctatcgccg tttttcctgc aaggttttat tttcatgtca cagtctacat ccgtgcttcg
    13321 tcgtaatgga tttactttta aacagttttt tgttgctcac gatcgctgtg cgatgaaagt
    13381 gggaacggat ggcattttgc tgggcgcatg ggcaccggtg gctggggtga aacgttgcct
    13441 tgatatcggc gcgggtagcg ggttgctggc actgatgctg gcgcagcgaa ccagtgacag
    13501 cgtgataatt gatgccgttg agctggaaag tgaagctgcc gcgcaggcgc aggaaaatat
    13561 caaccagtcc ccgtgggcag agcggattaa cgtccatacg gcggatattc agcagtggct
    13621 cacacagcag acagtacgct tcgatttaat catcagtaac ccaccttact atcagcaggg
    13681 agtagagtgc gcgacacctc aacgggaaca ggctcgctat accactacgc ttgatcaccc
    13741 atcgctgctg acctgtgcgg cggagtgcat taccgaagag ggatttttct gcgtggtgct
    13801 gccagagcag gttggtaatg gttttactga actggcatta agcatgggct ggcatttacg
    13861 tttgcggacg gatgtggcgg aaaacgaagc gcgactgccg catcgggtgc tgctggcatt
    13921 ctccccgcag gcgggagaat gctttagcga tcgcttagtc attcgtgggc cagaccagaa
    13981 ctattccgaa gcgtatacgg cgctgaccca ggctttttat ctgtttatgt aatgattgcc
    14041 ggggggaagg atcgatggac cggaatgcgt gagcagttcc gggtaatcca gcgtaaaatg
    14101 caggccacga ctctccttgc gcatcatagc acagcgaaca atcaactcgg caacctgtac
    14161 cagattacgc agctccagca aattattcga cacgcggaag tgggcgtaat attcgtctat
    14221 ttcctgttgc aacatattga tacgccgcag ggcgcgctca agacgttttg ttgtgcgcac
    14281 aatgccgacg taatcccaca taaacagccg tagctcgtgc cagttatgct gaattactac
    14341 ccgttcgtca gggttctcaa cgcggctttc atcccacggt ggtaacgtac tgacgccgtg
    14401 ggcatcaggc atacgtctgg tgatatcttc tgccgccgac cagccataga ccagacactc
    14461 cagcaatgaa ttcgaggcca tgcggttagc gccgtgtaag ccggtataac tcacctcgcc
    14521 aatggcatac aagccctcga cgtccgtacg cccatgatca tcaaccatta caccaccgca
    14581 ggtatagtgt gcagcaggca caatcggtac cggttcttgt gtgagatcaa tccccagccc
    14641 gagcaatttt tcataaatca tcgggaaatg ctggcgaata aaatcggcgg gcttatggct
    14701 gatgtcgagg aacatacaat ctgcgccgag gcgtttcatt tcatggtcaa tggcgcgggc
    14761 gacaatatcg cgcggggcca gttcgccgcg ctcatcaaaa tcgggcataa aacgcgtgcc
    14821 atccgggcgc ttgagataag cgccttcgcc gcgcagtgct tccgttaaca ggaaattgcg
    14881 tgcctgtggg tgatacagtg cggtaggggg gaactgatta aattcgagat t
//
LOCUS       DAJVQG010000101        14743 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJVQG010000101 DAJVQG010000000
VERSION     DAJVQG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14743
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.101"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            233..1897
                     /gene="tssM"
                     /locus_tag="OQM83_002766"
                     /pseudo
     CDS             233..1897
                     /gene="tssM"
                     /locus_tag="OQM83_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354198.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            1908..3260
                     /locus_tag="OQM83_002767"
     CDS             1908..3260
                     /locus_tag="OQM83_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HCS1520986.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQHTRKRLEPQPKSNAENAAQTTVRTLIYAPETLASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPIESLLTQYHDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAATGTTAEK
                     "
     gene            3284..3766
                     /locus_tag="OQM83_002768"
     CDS             3284..3766
                     /locus_tag="OQM83_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCS1520987.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            3810..4753
                     /locus_tag="OQM83_002769"
                     /pseudo
     CDS             3810..4753
                     /locus_tag="OQM83_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4763..5242
                     /locus_tag="OQM83_002770"
     CDS             4763..5242
                     /locus_tag="OQM83_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1520988.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMSFSFLNIHVILLTIIKLFLIKKTKVVNFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
     gene            complement(5379..6164)
                     /locus_tag="OQM83_002771"
     CDS             complement(5379..6164)
                     /locus_tag="OQM83_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1520989.1"
                     /translation="MNLRRVCCICVLFSLLAGCVSESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(6494..6570)
                     /locus_tag="OQM83_002772"
     tRNA            complement(6494..6570)
                     /locus_tag="OQM83_002772"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6534..6536),aa:Asp,seq:gtc)
     gene            complement(6703..7434)
                     /gene="dnaQ"
                     /locus_tag="OQM83_002773"
     CDS             complement(6703..7434)
                     /gene="dnaQ"
                     /locus_tag="OQM83_002773"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCS1520990.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            7499..7966
                     /gene="rnhA"
                     /locus_tag="OQM83_002774"
     CDS             7499..7966
                     /gene="rnhA"
                     /locus_tag="OQM83_002774"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCS1520991.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(7963..8685)
                     /locus_tag="OQM83_002775"
     CDS             complement(7963..8685)
                     /locus_tag="OQM83_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS1520992.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            8719..9474
                     /gene="gloB"
                     /locus_tag="OQM83_002776"
     CDS             8719..9474
                     /gene="gloB"
                     /locus_tag="OQM83_002776"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCS1520993.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            9546..10904
                     /gene="mltD"
                     /locus_tag="OQM83_002777"
     CDS             9546..10904
                     /gene="mltD"
                     /locus_tag="OQM83_002777"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCS1520994.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(10953..11549)
                     /locus_tag="OQM83_002778"
                     /pseudo
     CDS             complement(10953..11549)
                     /locus_tag="OQM83_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(11580..12380)
                     /locus_tag="OQM83_002779"
     CDS             complement(11580..12380)
                     /locus_tag="OQM83_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCS1520995.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            12621..13535
                     /locus_tag="OQM83_002780"
     CDS             12621..13535
                     /locus_tag="OQM83_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1520996.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(13532..14335)
                     /gene="dkgB"
                     /locus_tag="OQM83_002781"
     CDS             complement(13532..14335)
                     /gene="dkgB"
                     /locus_tag="OQM83_002781"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCS1520997.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(14498..14574)
                     /locus_tag="OQM83_002782"
     tRNA            complement(14498..14574)
                     /locus_tag="OQM83_002782"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14538..14540),aa:Asp,seq:gtc)
BASE COUNT         4062 a         3480 c         3498 g         3703 t
ORIGIN      
        1 ggaacagacc tatctgcaac tgctggagca acgttacctg ccctcgctgt ttaacgggct
       61 ggtcaaagcg atgaacgccg cgccgccgga gagtgaagaa aaactcgcgg tgctgcgcgt
      121 gatgcgaatg ctggaggaca aaagcggacg taacaatcag gtggtgaagc agtatatggc
      181 aaaacgctgg agcgaaaagt tccacggtca gcgcgatatc caggcacaac tgatgtccca
      241 tcttgactac gcgctggctc atactgactg gcacgcagag cgtcaggcgg gcgacggtga
      301 cgccatcagc cgctggacgc catatgacaa gcccgtggta tcagcacaga aagaactgag
      361 caaactgcct gtctaccagc gggtttacca gagcctgaaa acgcgggcgc tgggcgttct
      421 tcctgccgac ctcaatctgc gtgaccaggt agggccaacc tttgaccagg tgtttacatc
      481 tgccgatgac aacaaactgg ttgttccaca gtttatcacc cgctacggcc tgcaaagcta
      541 ttttgtaaaa cagcgcgatg aactggttga actgacagcg atggattcct gggtacttaa
      601 cctcacccgc aatgtgaaat acagtgacgc cgaccgtgcg gaaatccagc accagttgac
      661 cgagcagtat atcagcgact acaccgccac ctggcgggct gggatggaca atctgaatat
      721 ccgcaatttt gagtccatcg gacaactgac cggggcgctg gagcaggtta tcagcggcga
      781 ccagcctttg cagcgggcgc tgaccgtgct gcgtgacagc acacagccag gcgtcttttc
      841 tgaaaaactc tctgccaaag aacgggagga agccctggca gagccggatt accagttact
      901 cacccgcctc gggcatgaat tcgccccgga aaacagtacc ctggcagtac agaaagacaa
      961 agaaagcacg atgcaggccg tgtatcagca actcaccgag ttgcaccgct acctgctggc
     1021 aatccagaac gcgcctgtac cagggaaatc ggcgctgaaa gccgtgcagt tacggcttga
     1081 tcagaacagc agcgatccga tattcgccac ccgccagatg gcaaaaacgc tgcctgctcc
     1141 gctcaaccgc tgggttggca gactgactga ctaggcctgg catgtggtga tggtggaggc
     1201 tgttcattat atggaagtgg actggcgcga cagcgtggtg aaaccgttta acgagcaact
     1261 ggcaaataac tatccgttta atccgcgttc tgcacaggat gcctcactgg atgccttcga
     1321 acgcttcttt aaaccggatg gcatactgga taccttctac cagcagaacc tgaagctgtt
     1381 tatcgataat gacctgagtc tggaggatgg cgataacaac gtcattattc gcgaagatat
     1441 tattgcgcaa ctggaaactg cgcagaaaat ccgtgacatc ttcttcagca aacagaacgg
     1501 tctgggaaca tcctttgccg tggaaacggt atcgctttca ggcaataaac gccgcagtgt
     1561 actgaacctt gacagtcagt tagtcgatta cagccagggc cgtaactata ccgcccatct
     1621 ggtctggcct aacaacatgc gcgaaggcaa cgaaagtaag ctgacgctca tcggcaccag
     1681 cggcaacgcg ccgcgcagta tcagcttcag cgggccgtgg gcgcagttcc gcctgttctg
     1741 ggccggacaa ctgaccggag tacaggatgg caactttacc gtgcgcttta gcgtggacgg
     1801 tggcgcgatg acctaccgtg tgcataccga cacggaagat aacccgttca gcggtgggtt
     1861 gttcagccag tttggtctgt cagacacact gtactgagga ggcagggatg aatagtaacg
     1921 tactgacaca aactatcgtt accggcagtg acccgcgcgg gctgccggaa ttcagcgcca
     1981 tccgcgagga aataaacaaa gccagccacc cgtcacagcc tgagctgaac tggaaactgg
     2041 tggagtcgct ggcgctggcg atttttaaag ccaacggtgt ggatttacac accgccacct
     2101 actatacgct tgcccggaca cggacacagg gactggcggg attctgcgaa ggtgcggaac
     2161 tgctggcggc aatggtaagc cacgactggg ataagttctg gccgcagggc ggcccggcgc
     2221 gtactgaaat gctggactgg tttaactccc gaaccggcaa tattctgcgt cagcaaatct
     2281 cctttgcgga atccgaccta ccactgatct accgcacaga gcgggcattg cagcttatct
     2341 gcgataagct ccagcaggtg gaactgaagc gcgttccgcg cgtggagaat ctgctctatt
     2401 ttatgcagca cacgcgtaaa cggcttgaac cccagccgaa gagtaacgct gagaacgccg
     2461 cacagaccac ggtcagaacg ctgatttatg ccccggaaac actggcatct tccacaccag
     2521 aagcggtagt gcctcccctg cccggcctgc ctgagatgaa agtggaagtg cgcagtctga
     2581 cagagaatcc cccacaggcc agtgtgataa agcaaggcag tacggtaaga gggtttatcg
     2641 cagggatcgc ctgttcagtg gctgtcgcct cagcattgtg gtggtggcag gtctatccgg
     2701 tgcagcagca actgttacag gttaacgaca ccgctcaggg cgcagcaacg gtgtggatgg
     2761 cctcacctga actcgaaaac tatgagcgca ggctgcaaca acttcttgat acctccccgg
     2821 tacagccgct ggaaaccggg atgcagatga tgcgtgttgc cgacagtcgc tggccggaaa
     2881 gcctgcaaca gcaacaggcc tcgacacaat ggaatgaggc actcaaaacc cgcgcacaga
     2941 gtagcccgca gttgcgtggc tggttgcaga cccgccagga cttacatgct tttgcagatc
     3001 tagtgatgca gcgcgagaaa gagggactaa ccctttccta tatcaaaaat gtcatctggc
     3061 aggcggagcg gggactgggg caggaaaccc ccattgagtc tctgttgacg cagtaccatg
     3121 atgcccgtgc gcagaagcag aatacagatg cgctggaaaa acaaattaat gagcgactcg
     3181 aaggcgtgtt aagccgctgg ctgctgctga agaataacgt catgccagag gcggcaaccg
     3241 gtaccacggc cgaaaaataa ttatttaatt aaaggagatt atcatggcaa atataagtta
     3301 tttatctttg agtggagaga cacaagggct aatttcagca ggttgctcaa cgcttgattc
     3361 tgtcggaaac aaagcgcagc cagaacataa agatcagatc atggtttatg cgttaatgca
     3421 ttccatctca agaagtcaaa atgtcaatca tcatgaatta attatcacca aaccggttga
     3481 taaatcctca ccattgctgg caaaagcact tagtgacaat gaaaaaatgg caatatgtga
     3541 atttatactc taccgaacca gtaaagcggg tatttaccag ccttattata aaataaatct
     3601 gtcaaaagcc cgaatatcaa gtattgattt tgtcacaccg catgctgtcc tggaaaaaga
     3661 actggaacca caggagagaa ttgcctttat ttatgaagat atttcctggg agcatacgtt
     3721 agccggtacg aatgcaatga gtaaatggca ggatcgtgta cagtaactta ctgtaataac
     3781 ataataaatt actattgcgg agagataaaa tggctggttt aaggaataac aacaacacac
     3841 agaatgcaca gtgggctgat tatgtgggtg acattcttcg gggggctcag ccgataaacc
     3901 agctagtacc tcagcaccct tatcttaacg acgtcccact cattgatgag ttaaggcatc
     3961 agaacactca tcatgttatt ctcttgacac ttgatgttgc aaaaaaaaaa aaaaaaaaaa
     4021 aaaaaaaaaa aaaaaaatac tcagcccaat aacatcattt gattacattc attttatcac
     4081 tacacatcct tcaggtataa aagatacact cgcctggtta gttaacgcga gtaaattaat
     4141 gactgagttt gatgacaatg ggaaaattat atttaacatt aatgccttaa agtatacaaa
     4201 agcaagttac tttgaaatac ttggagagaa atatataaaa atcacaacat ccagcccctg
     4261 gcttttagaa aagctcggta aatatatttt ctcttcacga gcgcctcaag ttctggaact
     4321 cgctattggc tggcgaggag ctttatctga atcaataaaa ggagttaagt tctgtatctg
     4381 gttttcagta gcctggagaa ccatagaatt tattatgagt tcagagcgag atctggttaa
     4441 ttttcttggc gatttttcaa tggatgtggc caaagcagtt atagccggtg gtgttgcaac
     4501 cgctattgga agcctggctt cttttgcctg tgttagcttt ggctttccag taattcttgt
     4561 cggaggagca attttactga cagggatagt gtgtacagtt gttttaaatg aaatcgatgc
     4621 tcaatgccat ttatcagaaa aattaaaata tgcaattaga gatggactaa aacggcaaca
     4681 ggaacttgat aaatggaaaa gggaaaacat gactccattt atgtatgttc ttaacactcc
     4741 acccgtgata tgaggattaa ccatgcgttt acatgttaaa ttaaaagagt ttctttcgat
     4801 gttttttatg gccatattat ttttccctgc attcaatgct tctttgtttt ttaccggagt
     4861 taaacctcta tattcaataa taaaatgctc cacggaaata ttttatgatt ggagaatgtt
     4921 gattctgtgc tttggtttca tgtcgttttc ctttttaaat attcatgtaa ttttacttac
     4981 gataataaaa ttattcttaa tcaaaaaaac aaaggtagtc aatttcgcca cagacataac
     5041 aatacagcta actcttatat ttttactaat agctatcgta atagcacctt taatagctcc
     5101 ttttgtcaca ggatatgtca atgctaatta tcacccctgc ggtaataata cgggaatttt
     5161 cccaggagca atctatatta aaaatggcat gaaatgtaat aatgaatata tttctcgcaa
     5221 ggaagattca gcagttaaat aattaaacat tctaaaacaa acagttttta actccggatt
     5281 ccatgtcctt tcttgcactg tctttgtcaa gaaaaatttc ttatgcaaag aattttgtat
     5341 tatttccgcc agaaattcct ggcggcagat ataatttatt attgatagcg gttaaccaaa
     5401 tcttttacca tcgctttata ggtcttttgc ttaagttgca tgatttgctg ctctgtcata
     5461 tcagtcgtac tggcttcatt cttgtcgggc aatggtggaa gcactttata ttcataattc
     5521 accggcgacc aggcagccca ctcacctgta gctacatcca ccagcgtgaa tcgtaccaga
     5581 tagcgcagtg acatcgtgtc agtcaatttt tgatttttat aatctgacca aactattgat
     5641 ttcaatccag agtcatattt tcctgtctgc aacgtatcct ggaaaacaat aatcgctttc
     5701 tgatgccctt ttgcagccac aaaacgcagt gcctgcatcc agttcatatt ctcagcactg
     5761 gcaacatcct cgttctcatt tttatctttg tttttgttac aagtcagagg cttttgcctg
     5821 tccgggatac cagagaatgt ggaaacagta taatattttc gcatctcctc ctgcataatg
     5881 gtttccggtg cgcggttgcc ggattgaaca agtattacag gagaattaag gggaacgcgg
     5941 aactcatctc catcaagcgc agcttgaata tcctcttcgg atacggccag agtggtttca
     6001 ttaccgaata aatctttgtc tgtcagttgc tggggagtgt ttttattaat attactttgt
     6061 gtgacttgag cttttttctt cttttcatca atagaagatt cagagacaca tcctgcaagc
     6121 agcgagaata atacacatat acaacaaacc cttcttaaat tcataatcgg acactccctc
     6181 gcctgtcata atcatggtaa tgtttagcta acgattttta attatatact ccacttgaat
     6241 cagaacaaac aaaaataaca gtatatataa attcattaaa tcaatagaaa aatgaaaatc
     6301 ataacattca aatatattta aattgaatga tttgatagta tgattaaata ttaatgatat
     6361 ggtctaccat atgtgattaa cacatttaca gcataacaaa accatagacc gaagaaatag
     6421 cgggatttaa atgcaaaaaa accgcaaaat taaaaatctt gcggttctct gaactcattt
     6481 tcatgagtga atttggcgga acggacggga ctcgaacccg cgcccccctg cgtgacaggc
     6541 aggtattcta accgactgaa ctaccgctcc gcgttgtgtt ccgttgggaa cgaggcgaat
     6601 attacggatt gcctcaccct tcgtcaacgg tttttctcat cttttgaatc gtttgctgca
     6661 aaaatcgccc aagccgctat ttttagcgcc ttttacaggt atttatgccc gccagaggca
     6721 gcttccgccc ttcttctcca ccagatcaag acgggcttca tgagctgcaa gctcttcatc
     6781 tgtcgcaaaa acaacgcgta acttacttgc ctgacgtaca atgcgctgaa ttgttgcttc
     6841 accttgttgc tgctgtgtct ctccttccat cgcaaaagcc atcgacgttt gaccaccggt
     6901 catcgccaga taaacttccg caaggatctg tgcatcgagt aatgccccgt gtagcgttcg
     6961 tttactgtta tctatttcgt agcgggcaca taacgcatcg aggctgttgc gcttaccagg
     7021 aaacattttc ctcgccaccg caaggctatc ggtgacctta cagaaggtat tggtcttcgg
     7081 aatatcgcgc ttaagcaacg aaaactcgta gtccataaag ccgatatcga acgctgcgtt
     7141 atggatcacc aactccgcgc cgcggatata gtccatgaac tcatcggcta cttcggcaaa
     7201 cgtgggctta tcgagcaaaa attcatcggc aataccatgt acgccaaagg cttccggatc
     7261 caccagccga tcgggtttga gataaacatg gaagttattg cccgtcaggc gacggttcac
     7321 cacttcaacg gcaccaatct caatgatctt gtggccttca tagtgcgcac caatctggtt
     7381 cataccggtg gtttcggtat cgagaacgat ctggcgtgta attgcagtgc tcatagcggt
     7441 catttatgtc agacttatcg ttttacagtt cgattcaatt acaggaagtc taccagagat
     7501 gcttaaacag gtagaaattt tcaccgatgg ttcgtgtctg ggcaatccag gacctggggg
     7561 ttacggcgct attttacgct atcgcggacg cgagaaaacc tttagcgcag gctacacccg
     7621 caccaccaac aaccgtatgg agttgatggc cgctattgtc gcgctggagg cgttaaaaga
     7681 acattgcgaa gtcattttga gtaccgacag ccagtatgtc cgccagggta tcacccagtg
     7741 gatccataac tggaaaaaac gtggctggaa aaccgcagac aaaaaaccag taaaaaatgt
     7801 cgatctctgg caacgtcttg atgctgccct agggcaacat caaatcaaat gggaatgggt
     7861 taaaggccat gccggacacc cggaaaacga acgctgtgat gaactggctc gtgctgcggc
     7921 aatgaatccc acactggaag atacgggcta ccaagtcgaa gtttaagctt gtggtttacg
     7981 acattgccgg gtggctccaa ccgcctggcg aattcgtggc ttgtttttac tctgtttcat
     8041 cgggtttagc gttaaaggaa tagtccgttt ccgggcaaca ataagttgta agcaaccaag
     8101 cgcaggaata tgcgcattca atagttttcc tccgtgtttg ttccacggga gaacgtggaa
     8161 acggctggcg tgcagcactt caaaattcaa caaagagagc cagtccagtt gccgcatcag
     8221 agtaaacatc cggctgttat agggcgaggt ttttcgcaat accggcacaa gtttgcgtaa
     8281 tcccattaaa ctaatgggat tgaagccact aatcaccagc cagccatcat caatcaatac
     8341 ccgatcggtt tcacgcaata aacgatgcgg atcggtgcac cacggcaatg tatgtgccag
     8401 tagacaaaca tcaacggatt tatcggcaaa aggaagatga agtggatccg cctgcacctg
     8461 gacgggcatt ccttgcgcag aaacattcac ttgatgagaa accgcgcacg cttcgcaatt
     8521 gatttctgcg cttaaattgc caatcttaag cagatgaaaa ccatacattt tagtgaacca
     8581 cgggttgagc tggcgctcca gcgccttgcg ataaagctct ccccagggca aatcgcccca
     8641 gcgatcagga gccacgacag tttgagggac tcttgccggt ttcatcacaa ccttccgttt
     8701 cacactaaga ggtaatctat gaatcttaac agtattcccg cctttgatga caattacatc
     8761 tgggttttga atgatgaagc aggtcgctgc ctgattgtcg atcccggaga cgcagagcca
     8821 gtattaaacg ccattgccgc caataactgg caaccggagg ccatatttct cacccaccat
     8881 catcacgatc acgttggcgg cgtaaaagaa ctggtggaaa agtttccgca aattgtggtg
     8941 tatggtccac aagagacaca agataaggga acaacacagg tagtcaaaga tggcgaaact
     9001 gccttcgttt tggggcatga atttagtgta attgccacac cgggtcacac tttaggacat
     9061 atctgttact tcagtaaacc ttatctattt tgcggcgaca cgctgttttc tggtgggtgt
     9121 ggtaggttgt ttgaagggac agcatcacaa atgtatcaat cacttaataa gttaagtgcg
     9181 ttacctgacg atacattggt atgttgtgct catgaatata cgttatcaaa tatgaagttt
     9241 gctttgagta ttcttccgca cgatttgtcc ataaatgatt attatcgtaa agttaaggag
     9301 ttacgggcaa aaaatcaaat aacactaccc gtaattctga aaaatgagcg gcaaattaat
     9361 gtttttttaa gaacggaaga tattgattta attaatgtaa ttaatgaaga aacattattg
     9421 caacaacctg aagagcgttt tgcatggtta aggtcaaaga aagataggtt ctgataaaac
     9481 tttcttgtca tcggctccgt tcgccgttat gatcggtcgt cttttaagca actattgaca
     9541 cacacatgaa ggcaaaagcg atattactcg cctctgtcct gctcgtgggt tgccagagta
     9601 ccggcaacgt tcaacagcac gcacagagcc tttctgcagc tggtcaaggg gaagcagcaa
     9661 agtttacaag tcaggcacga tggatggacg atgggacgtc tatcgcgcca gatggtgact
     9721 tgtgggcttt cattggcgac gagctaaaga tgggaattcc ggaaaatgac cggattcgcg
     9781 aacagaaaca gaaatattta cgcaataaga gctatctcca cgatgtaact ttacgggcag
     9841 agccgtatat gtactggata gcagggcaag ttaaaaaacg taacatgcct atggaactgg
     9901 tactactacc catagtggag agcgcttttg atcctcacgc aacgtctggc gccaatgccg
     9961 caggcatctg gcagatcatt ccgagcacgg ggcgcaatta tggtttgaaa cagacccgca
    10021 attatgacgc gcgtcgcgat gttgttgctt caacaactgc cgcgctgaat atgatgcagc
    10081 gtctgaacaa aatgtttgac ggcgactggc ttctgaccgt agcggcttat aacagcggcg
    10141 aaggtcgggt catgaaggca attaaaacga acaaagcgcg tgggaaatcc acggacttct
    10201 ggtcgttacc gttgccgcag gaaacgaagc agtacgtgcc taaaatgctg gcattgagtg
    10261 atattctcaa aaacagcaag cgttatggcg tacgtctgcc aacgaccgat gaaagccgtg
    10321 ctctggcgcg tgtgcacctg agtagcccgg ttgaaatggc gaaggttgca gatatggcgg
    10381 ggatttccgt cagcaagctg aagacattca acgctggcgt gaaaggctcc acgctgggcg
    10441 caagtgggcc gcagtacgtg atggtgccaa agaagcatgc agatcaactg cgtgaatctc
    10501 tcgcttcagg cgaaattgct gctgtacagt cgacgctggt tgccgacaat acgccgctta
    10561 acagccgtgt ttacaccgta cgctctggcg acacgctttc aagcatcgct tcacgtctcg
    10621 gcgtaagcac caaagatttg cagcagtgga acaaactgcg cggatctaag ctgaagccag
    10681 gccaaagttt gacgattggt gcaggcagta gcgcacagcg gttggcaaac aacagcgata
    10741 gcattacgta tcgtgtgcgc aaaggcgatt cgctttcaag cattgctaaa cgccacggcg
    10801 tgaacatcaa agatgtgatg cgctggaaca gcgacactgc gaatctgcaa ccaggcgata
    10861 agctgacgtt gtttgtgaaa aacaacaaca tgccagattc ctgacaaacc agataataaa
    10921 aaaggcaccg attcccccgg tgcctttttt atttatgccg ctttatgtgc ttctaccatg
    10981 atgatatcac tggtgaaaga gccatcagtc tgcaaggaaa aatacgttct cacctctgtc
    11041 gatgcgctct gttggtaaat gcgaatagcg tctactaacg cttctggcgt acgcattctc
    11101 acgacccatg aagaaaattc cagcggtaac ttatctgtaa ttaaattatc aactatcaga
    11161 ttggcttcat tgattaacgt caaccacgca ccgctggcgt agtttcgtac gtgagaggta
    11221 tcgcgtaatg cttctaccgc ctgtaaccag atgtcgcgca ctgggtgacc cggagacatt
    11281 acatccatca caatcagcct accgccaggt ttcaatatcc tattcacttc tcgcagtgct
    11341 gcaccaacat catgccagtg atgggcagaa taacggctga taacaatatc aaatgcgtta
    11401 tcggcaaatg gcagacttgc ggcatatccc tggcgggtgg tgatattttt cagttgccgg
    11461 gcttcggcag cttgtgccac gacatccagc atgtgggcag ataagtcata cgccaccacc
    11521 gcgctcacgt tttgcgcagc gccaaacgtt ggtctgccct gcggcagacc tgacatacct
    11581 tatttatcag gcttgccggg actgaattca acgagtagcg gattgtgatc ggaagcgcgc
    11641 gtaaccagta cagaagcttc actgacgttc agaccacggt agaaaacaaa atcgagcggg
    11701 cgaccaaacg cccgacggcg ctgatcatcg gtaaaacgca cctggcgcag cgacatttcc
    11761 cgcgcaaagc gatataacgc gttcatcctt ctacggctcc aggcattgaa atctcccgcc
    11821 ataatgaccg ggccgctgtg gtgagctatc tgatcaccaa taggaagtaa ctgcttacta
    11881 tagacatcca cgcccagact gaagttgacg gcgtgtatat taaccaccat caacaggcgg
    11941 gtgtcaggta atggatagac cgtcaccagt gccgacttcg ccagacgcaa aatgggttct
    12001 cgttcacgta acgggcagca atacactgga tgtgccgccg aaagggtcat tacgccagaa
    12061 ggatgttgtg gcagcacgaa agcgggtacc tgatcggcgg caagatagtt agcggtcgca
    12121 aactgtacca actctggcgt tgtttgcgct tcctgcaata acaccagatg tgcgtctttg
    12181 ccgtaattct ttaataccga caaccattca gcgcgttgct gtttgtatat gttccacacc
    12241 aggatccgaa tacgctcttc ggtacttaac ggttctccgg gtggtaatgc ctggccgatg
    12301 ctcgcaaaag accccggcgg taagatcctc tccgcaggtt gtccggcaac atagcgcatg
    12361 gcataggtgt tttttcgcac ttttgacttt gaaacctcta ttacgtcaac ccttccgcac
    12421 tcgggagggt tctactgtta tagggatttt agctcactct ttcaacgagc aattgtatat
    12481 tgttatgtaa gcaagtgctt acaccggtca gaaatttgct cattattgag aacattaaat
    12541 cggatgacat atcacgcatc ctgatggttt aatgacaaat aaattcaaaa tatctcatct
    12601 attttgcaac aggagcaaaa atgaaagcca cgtcggaaga actcgccatt tttgtttcgg
    12661 tcgtcgaaag cggcagcttt agccgggcag cggaacaatt agggcaagca aactcagcgg
    12721 taagccgggc ggtgaaaaag ctggaaatga aacttggcgt tagcctgctt aatcggacca
    12781 cgcggcaact tagcctgacg gaagaaggcg agcgttattt tcgtcgcgta cagtcaattt
    12841 tgcaggagat ggcagcggca gaatcagaaa ttatggagac gcgtaataca ccgcgtggac
    12901 tgttacggat cgatgccgca actccagtgg tgctgcactt tctgatgccg ttaattaagc
    12961 ctttccgtga acgctatccg gaagtcactt tatcgctagt ctcctccgaa acgattatta
    13021 atttgatcga aagaaaagtg gatgtcgcga tacgcgctgg tacgttaacg gactccagct
    13081 tacgtgccag gccgttattt aacagttatc gaaaaattat cgcctccccc gattatattt
    13141 cccgctacgg gaagccagaa acgatcgacg atttaaagca acatgtttgc ctgggattca
    13201 ctgaacccgc ttccctcaat acctggccga tagcctgtag tgatggacaa ttacatgagg
    13261 tgaagtacgg tttgtcatcc aatagtgggg aaacactgaa acagctttgc ctgagtggca
    13321 acgggattgc gtgtttgtcc gactacatga tcgacaaaga aatcgctcgc ggagaattgg
    13381 tggagttaat ggcagataaa gtgttgccag tggaaatgcc attcagtgcc gtctattaca
    13441 gcgaccgtgc ggtaagtacg cgcatccggg cttttatcga tttccttagc gagcatgtaa
    13501 aaacagctcc cggaggagct gtcagagagg cttaatccca ttcaggagcc agaccttccg
    13561 ggctaaccag gcggtcgttg caatccagtg cggcgatcgc ttttttatct tcggcatcaa
    13621 gctgtaaatt ttgtgcctta agattacttt ccaggttttt acgtttagta gaagaaggaa
    13681 ttactgagta accttccccc atagcccacg ccagaatcac ttgtgccgga gtcgcattgt
    13741 gtttagctgc aatacgagca ataacctcat ctttcagtgc cttaccataa gccagcgtca
    13801 tataggaagt gatatgaata ccgtgctgtt tagcccaggc aaccacttta cggttttgca
    13861 gataaggaga gagttcaatc tggttggtag cgatgttttc agcgccaaca gcagcaatcg
    13921 ctttttccat caacgggatc gtgaagttgg aaataccgat ctcacgtgtc agaccttgtt
    13981 ttttggcctc cagcagcgcc tgcataaact cttcaacaga gacttcatcg tttggtgacg
    14041 gccagtggat tagcgtcaga tcaacataat cggtacgcaa tttttgcagg ctctctttta
    14101 gactcgggat caatttgtct ttgctgagat tttcaatcca gattttagtg gtgatgtaga
    14161 gttcatgacg tggcacgcca ctttctgcaa tcgcctgacc tactgcggct tcgttatcat
    14221 agatttgtgc agtatcaatt gcgcgataac caagttcaag cgccgttttc acagatgaaa
    14281 taacaacgtc gtctttgaga cggaaagtac ctaaaccaaa tgcagggata gccataatat
    14341 gcctctttta atgctatttt cgttaactga ggtgattatg ggcttcagac tgttgcggaa
    14401 aaagagcata aaatgcagag gatttttgcg attctggcaa taatagatat acagacgaaa
    14461 aaaaacctcg agcgttaact cgaggcttct taataagtgg cggaacggac gggactcgaa
    14521 cccgcgaccc cctgcgtgac aggcaggtat tctaaccgac tgaactaccg ctccaccgaa
    14581 ttcttttaca accaccggtt ttatgaccgg cttactgctt aatttgatgc ctggcagttt
    14641 atgaatcact tcgggctgtt gcttcgcaac gttcaaaagc tttcgctttt gtcctactct
    14701 cttatcgagt agtgaactgc ctcaaaggtc agtgccattc ttc
//
LOCUS       DAJVQG010000102        14708 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJVQG010000102 DAJVQG010000000
VERSION     DAJVQG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14708
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.102"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            254..2020
                     /gene="adeD"
                     /locus_tag="OQM83_002783"
     CDS             254..2020
                     /gene="adeD"
                     /locus_tag="OQM83_002783"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCS1520998.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDVEKFAFTTLEVTE"
     gene            complement(2065..3456)
                     /gene="uhpT"
                     /locus_tag="OQM83_002784"
     CDS             complement(2065..3456)
                     /gene="uhpT"
                     /locus_tag="OQM83_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCS1520999.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3594..4913)
                     /gene="uhpC"
                     /locus_tag="OQM83_002785"
     CDS             complement(3594..4913)
                     /gene="uhpC"
                     /locus_tag="OQM83_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCS1521000.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4923..6425)
                     /gene="uhpB"
                     /locus_tag="OQM83_002786"
     CDS             complement(4923..6425)
                     /gene="uhpB"
                     /locus_tag="OQM83_002786"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCS1521001.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6425..7015)
                     /gene="uhpA"
                     /locus_tag="OQM83_002787"
     CDS             complement(6425..7015)
                     /gene="uhpA"
                     /locus_tag="OQM83_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCS1521002.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(7090..7380)
                     /gene="ilvN"
                     /locus_tag="OQM83_002788"
     CDS             complement(7090..7380)
                     /gene="ilvN"
                     /locus_tag="OQM83_002788"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCS1521003.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7384..9072)
                     /gene="ilvB"
                     /locus_tag="OQM83_002789"
     CDS             complement(7384..9072)
                     /gene="ilvB"
                     /locus_tag="OQM83_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCS1521004.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(9178..9276)
                     /gene="ivbL"
                     /locus_tag="OQM83_002790"
     CDS             complement(9178..9276)
                     /gene="ivbL"
                     /locus_tag="OQM83_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCS1521005.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9841..9930
                     /gene="tisB"
                     /locus_tag="OQM83_002791"
     CDS             9841..9930
                     /gene="tisB"
                     /locus_tag="OQM83_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCS1521006.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            10210..11394
                     /gene="emrD"
                     /locus_tag="OQM83_002792"
     CDS             10210..11394
                     /gene="emrD"
                     /locus_tag="OQM83_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCS1521007.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11402..11899)
                     /locus_tag="OQM83_002793"
     CDS             complement(11402..11899)
                     /locus_tag="OQM83_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCS1521008.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11896..12258)
                     /locus_tag="OQM83_002794"
     CDS             complement(11896..12258)
                     /locus_tag="OQM83_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCS1521009.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12248..12595)
                     /locus_tag="OQM83_002795"
     CDS             complement(12248..12595)
                     /locus_tag="OQM83_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCS1521010.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12704..13153
                     /locus_tag="OQM83_002796"
     CDS             12704..13153
                     /locus_tag="OQM83_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521011.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13402..14610)
                     /locus_tag="OQM83_002797"
     CDS             complement(13402..14610)
                     /locus_tag="OQM83_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1521012.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSLPGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPAYTSQRC
                     VCCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
BASE COUNT         3473 a         4051 c         3800 g         3384 t
ORIGIN      
        1 tcccatgctg aggtagttta aataatcgcg aaagcgtccc tgattcatta ctcacgtaat
       61 cggtattgtc attattcatt tttttgtcca ttggaggaga tttaatccct tgcttttgta
      121 aaacaaatga caacaacggt tcagtgataa ttatcacatt tcaattgcac attaatggat
      181 attctttaat aatctcgcga cgtttcttta tgataaataa taatcaaatt gataaaatca
      241 aaatgagaaa aatatgaata attctattaa ccataaattt catcacatta gccgggctga
      301 ataccaggaa ttgttagccg tttcccgtgg cgacgctgtt gccgattata ttattgataa
      361 tgtctctatt ctcgacctga tcaatggcgg agaaatttcc ggcccaattg tgattaaagg
      421 acgttacatt gccggtgttg gcgcagaata cgctgatgct ccggctttgc agcggattga
      481 tgctcgcggc gcaacggcgg tgccagggtt tattgatgct cacctgcata ttgaatccag
      541 catgatgacg ccggtcactt ttgaaaccgc taccctgccg cgcggcctga cgaccgttat
      601 ttgcgaccct catgaaatcg tcaacgtgat gggagaagcc ggattcgcct ggtttgcccg
      661 ctgtgccgaa caggcaaggc aaaaccagta cttacaggtc agctcttgcg tacccgccct
      721 ggaaggctgc gatgttaacg gtgccagttt tacccttgaa cagatgctcg cctggcggga
      781 ccatccgcag gttaccggcc ttgcagaaat gatggactac cctggcgtaa ttagcgggca
      841 gaatgcgctg ctcgataaac tggatgcatt tcgccacctg acgctggacg gtcactgccc
      901 gggtttgggg ggtaaagaac ttaacgccta tattactgcg ggtattgaaa actgccacga
      961 aagttatcag ctggaagaag gacgccggaa attacaactc ggcatgtcgt tgatgatccg
     1021 cgaagggtcc gctgcccgca atctcaacgc gctggcaccg ttgatcaacg aatttaacag
     1081 cccgcaatgc atgctctgta ccgatgaccg taacccgtgg gagatcgccc atgaaggaca
     1141 catcgatgcc ttaattcgcc gcctgatcga acaacacaat gtgccgctgc atgtggcata
     1201 tcgcgtcgcc agctggtcga cggcgcgcca ctttggtctg aatcacctcg gcttactggc
     1261 acccggcaag caggccgata tcgtcctgtt gagcgatgcg cgtaaggtca cggtgcagca
     1321 ggtactggtg aaaggcgagc cgattgatgc gcaaacctta caggcggaag agtcggcgag
     1381 actggcacaa tccgctccgc catatggcaa caccattgcc cgccagccag tttccgccag
     1441 cgactttgcc ctgcaattta cgcccggaaa acgctatcgg gtcattgacg tcatccataa
     1501 cgaattgatt acgcactccc actccagcgt ctacagcgaa aatggttttg atcgcgatga
     1561 tgtgtgcttt attgccgtac ttgagcgtta cgggcaacgg ctggctccgg cttgtggttt
     1621 gcttggcggc tttggactga atgaaggtgc gctggctgcg acggtcagcc atgacagcca
     1681 taatattgtg gtgatcggtc gcagtgccga agagatggcg ctggcggtca atcaggtgat
     1741 tcaggatggc ggcgggctgt gcgtggtacg taacggccag gtccaaagtc atctgccgtt
     1801 gcccattgcc ggcctgatga gcaccgacac ggcgcagtcg ctggcggaac aaattgacgc
     1861 cttgaaagcc gccgcccgtg aatgcggtcc gttacccgat gagccgttta ttcagatggc
     1921 gtttctttct ctgccagtga tccccgcgct aaaactaacc agtcaggggc tatttgatgt
     1981 cgagaagttt gccttcacta cgctggaagt cacggaataa taaaaaagcc cggcgtcatg
     2041 ccgggcaaaa gtcaccagtt acgtttatgc cactgtcaac tgctgaattt ttttctcgcg
     2101 gcggattttg cgttcttcca tcaccgccac tatcgccatc aggcagatac aaccaatcgc
     2161 ggcgatatca agtgcggcga aggtgcctgc ccagccggta aggccgaata ccggcgtccc
     2221 atcggcaatc attcccagac ctaacttggc aaagctgtca ccaatcaggt aagcaaaggt
     2281 gcctttaata ccatcggcag cgccaatcgc ttttttaggt acaaagccaa cagcagccac
     2341 accaatcaac aattgcgggc caaagaccag gaaacccaac gcaaagagag aagccagata
     2401 gatatattcg ttactggcat gttgatacac accgagcgtg gcgataatca gcgccagcgc
     2461 gatgcaggcc accaggccac ggcgaccgtt cgccaggtca gagagccagc cccacagcag
     2521 cgtaccgacc agcgcaccag cttcaaacag cgtaaagccc tgaatcgcca ccgctttaga
     2581 gagtttcagt tcctggaacg cgtatacggt tgaccactgg tcgataccaa tacgtaccac
     2641 atagaggaaa atgttggcga agcacagcag ccagatcact ttgtttttca gcacatactc
     2701 aacaaagatc tgccacttgg tcatatcggt agattctgtc tctttgtcct cttcgctgat
     2761 ctcctcgccg aacagttctt cagctttgcc gaggccataa gattccgggg agtcgctgcc
     2821 gtaacgcagg ccgataaaac cgacaatcag cgcgataatc gacgggaaga taaacatgcc
     2881 gatgacatgg ccatcgaaca ggtaatttgc cccgaacagc gccacgcctg ctgcgcctgc
     2941 accgccaagg ttgtgagaaa tattccagaa accgaggaat gtcccgcgtt tacgacgcgg
     3001 cgtccatttg gtgatggtgg agtaactgca cgaaccgccg gtactctgga aaaagccgct
     3061 taaggcgtag aaggcaatca tcaggaacag gctaaccgag ccgctgccca tactggcact
     3121 gaagcccagc atacaaatgg cagacaggat cagcatgaac ggcaggaatt gtttggtgtt
     3181 tttgccgtcg gcgtagtagg aaaccagcgt tttacccacg ccataagtga tggagaaacc
     3241 caggccgatc atccccagct gcgtcatgct caacccgtag gtcgagatca tgtcgttctg
     3301 cgcgatgtta aagttcttgc gaatcaggta catcgtcagg tagccgataa agaccaccag
     3361 gtaggattgc atgaacggtt tgaaccacat tttgcgccgc acttcgagcg gaaggtccag
     3421 ggtcggcttg cgaacctggt ttaagaaagc cagcatgggt tactcctgaa atgaatacct
     3481 gcgtgaggca tgcattgtaa aaatcagcag caaatcaggc ctgagatagc gtccaggcaa
     3541 aacctggaaa atttcttagt tttgccggat atgaagtgaa aaggtgagat gcatcacgct
     3601 tcgcgcggtg tctgggcgtt caaaaagggc agtaacagca gtgcggaaat cccggcggcg
     3661 atagagataa ccacaaaaaa tccgctccag tgccaggtat cgagtacttt cgccagcggc
     3721 caaccagcaa gcgacgcccc cagataagca aacaagccga caaaccccgt cgccgcccct
     3781 gccgcctctt tgtgggaaca ctctgccgcc gccataccga ttaacatctg tgggccaaag
     3841 acaaaaaaac caatggtgaa gaagcaggtt gcctgcatca cgtagctggc aaatggcatc
     3901 agccacaggg agccgactga aagcaaaatt ccggcggcga aaatcaaatt catcggccct
     3961 cggttgccgt taaacaattt gtccgagccc caacccgcta ccagcgcacc gataaatcca
     4021 cccagttcaa acatcgtcac tgccgtattc gccgtgacca gatcgacgcc cagcgtctcg
     4081 gacatataca aattgcccca gtcgttgatc gccgcccgga ccacatagac cagcacatag
     4141 caaaacgaaa gcagccagat atacggattc agcaacacat atttggtgag gatctcttta
     4201 cgcgtcagcc ctgccccttc ttgttgttga gctatctcca gcgcgtcgtg tcgccattca
     4261 ccgaccgccg gtaaacctaa cgcctgcggg cgatcgcgca gccgccagca gagaaaaatt
     4321 cccacgacga tcgccataca accagcaatc atcatcccgg cacgccagcc gtaatgtagt
     4381 gcagccgctg ccatcacaat gggaatgagt gcgccgccga cgttatgcgc cgtgttccat
     4441 aatgcccacc aaccaccgcg ctcggtacgt gaataccagg ccgttaacag acgcgcacac
     4501 accggtgaac cccagccctg gaaaaaggcg ttcagcaccc agagtacggc aaacgcccat
     4561 agcgacgtcg agaagccaaa cagaatgttg ataatgcccg tggcgataag ccctatcccc
     4621 ataaaataac gggcatttga gcgatcgctg acaatgccgg agacaaactt cgacacgcca
     4681 taggtaatgt aaaacagggt cgctaacagg ccgatatcgc tacggctgag cacgccgtta
     4741 gcaaggattt ctggtacggc ggcgttaaaa cttttccgcg tgaagtaaaa cagcgcgtaa
     4801 cccagccaga tggtcagcag aatatgccga cgccagtagc gatagcgggc atcaatttca
     4861 tatttatcag ccattaatgg cgcatcggca ggcgctttca gaaacggcaa catcatcaaa
     4921 ccttagacat agcgttgagg tagagaaacg ctgacacgcg tgccgtgcag acaggaaatg
     4981 tgtaatgtgc cacccagcgc cgttacgcgc tcgcgcattc cggtgaggcc aaaaccttgt
     5041 tgcccggaac ccggcggcaa accgctgcca tcgtcttcaa taaccagcat caaccgttca
     5101 tcctgctgcc agccttgcag ggtgaccgcg ctggcatcag catgtttcac aatgttgttc
     5161 agcccttcct ggcagacacg aaacagcgtc acgcgctggt tttcgcttaa cgctgattca
     5221 tcgattcgcc attcgagatg gctgacaata ccgcgccctt ccagctccat ttcccgcatc
     5281 agtgagcgga tggcctgctc cagggtgaga tcatccaact ggcgcggacg taaccgaccc
     5341 aacaaacggc gcaccgcgtc gtaaacgccc agcgatagtt gttcgatgag ttgcccgctc
     5401 tgcttcacgc tggcgttatc tgccgccagc cgctgaacaa tgcccgcctg agtacgaata
     5461 gcagtgatgg tctggccgat atcatcatgc agctcacgcg ccacatcacg gcgcacgctc
     5521 tcttcggttt ccagcaaccg ttcagccaga tgctgattgc gcgccagttc cttttgcagc
     5581 gactggttaa gttcacgcaa ccgctggatg ccagcgccaa gcaacaaccc tgtcagactt
     5641 tgcaccagca gcgagagcaa taaatccacc ggatgatcgc gccaggtttg actggcgatc
     5701 agcgcgatgg cgttcatcaa cgtcgcaatc agcgcccctt gccaaccata gtgccaggcc
     5761 agcgcgataa tcggcagcac cagacagaat ggcgtaaagc gcgacagttc gtccggcaat
     5821 cccaactgga gccagagact gataacaaac agcagcaagt accagaccag atgtcgcccg
     5881 cgccagttga ttggctgaga aaccagtgac gggccgagcg gcagccaggt gttattggcg
     5941 agatagtgcc agaacaccag acatatcggg gccagcgtca ggccgccagt taaagtcagc
     6001 aacagcgcat tccacgactc tttgccgtgc caaagccagg gcagcgactg caacaacgcc
     6061 gccgccgtta acgccgcccc ctgtaacagc aaggtgcgcc agtcacgctg atggcgatag
     6121 cgcgagatca gcgctacggg cagtaacgtc agtaaactac cgatcatcaa taacggaaaa
     6181 tgggttaaac cgaccgcctg cgttagccag taaatcagca gccactccgc acccagcaat
     6241 acgggccagt atccgcgcgg gcattgcagc attagcccca gacgcagacc aaacggaaat
     6301 aacagcaccg ccatatcagg gcgctcaacc agatgcaggc tgatactcca caggcaaaac
     6361 catgcggcag agaagataaa aaagcaggca ataacggtaa ttaagcggga gaacaacgtc
     6421 ttcatcacca gccatcaaac atgcggcgcg ccagctctac gtcgttactg acgcccagtt
     6481 tttccatcag attggcgcga tggacgtgta ccgttttcgg tgacaagccc agttcggcgg
     6541 caatctcttt caccgccatt ccttgcgcca gtttttccgc cacctggcgt tcacgtttgg
     6601 ttagcgggtc ctgacgaccg gatgccagtt taatggcaat atccggcgtc agataacagc
     6661 cacccgtggc aaccgtatgc accgcagcaa tgagttcatc cgggctacag cgtttggaaa
     6721 gaaagccgcg tgcccccgcg ttaagcgcct gctcaaccag cgcaggactg tcgtgaacgg
     6781 agagcataat cgtcgccata cctttcggca gctggcttag cagctccaga ccggagatat
     6841 cgggcatgga gatatcgcaa atacacacct gcacaccgcg ccccggcagc cccgccagcg
     6901 cctcgcgccc cgaaccaaac tcggcaacta cctgcaaatc aggttccagc cccagcagct
     6961 gcgcaaagcc ggagcggacg atgaggtgat cgtctataag ggcaacggtg atcatggtct
     7021 tgtcctggcg ggtaaaaaaa aacgcgctta ccttaacgat aagcgcgatg ttgttcaagc
     7081 cttgagcggt tactgaaaaa acaccgcgat cttgttaaac atcgtcggat cggactgatt
     7141 acgctgcact ttcacgacat cttccagctt atcgatttgg cttatcatct gctccagacg
     7201 ctggtcgtca ttgaccagta gccagatatg gcttttgtcg ctgtcctgaa tcggcagaca
     7261 aagaatgcct tcaacgttaa aagcgcggcg ggcaaaaagg ccacaaacgt gggtcattac
     7321 gcccggatgg ttgcgaacgg tgagctccag aattacgttg tcatgagttg tgttttgcat
     7381 ggcttattcc cccaccattt cagtattcgc cgcacctggc ggcaccatcg ggtaaacttt
     7441 ttcttcggca tcaatgcgca catggatcag cgccgggcta gggcgattga tgatttcctg
     7501 caatgcagcc tgcggatcgg cttcgttatt caaatcacag gtttcgaggc cgaatccggc
     7561 ggcaatctgc ataaagttga ttttgcccgg ataggtggcg gcaaaaacgc cttgctcgta
     7621 gaacagactc tgttgctgat gcaccagccc cagcgcttcg ttgttcatca gaatgatttt
     7681 gacatccagc tgattttcac tggcggtcgc catctcctga atattcatca tcaggctgcc
     7741 gtcgccggag aaacacaaca ctttgcgatc cgggttcgcc agcgccgcgc caatcgccgc
     7801 aggcaggcca aaacccatcg tgcccagccc accggaggtc agccactggc gtgggcgatt
     7861 gagcggataa gcttgcgcgg tccacatctg atgctgaccc acatcggtgg tgataatcgc
     7921 attgtcatcg acacaggcgg caacggcgtt gatcaggccg taatggctta atggatcgca
     7981 cgctttcggg attggacacg ggaattcgcg ctgcaaatcc gctaccaact ggtgccactc
     8041 tgcacgcggt tgcgcttcca cctgcgggat caattgcgcc agcacgtcat caacatccgc
     8101 ctgaatcgcc acatgcggct gcttgatttt acccagctct gcgcgatcga tatcgacatg
     8161 aataattttg gcattcggac agaactgctc ggttttgcca atcgcccggt catcaaaacg
     8221 cgcaccgagc acaatcaaca aatccgcctc ctgcaaaata tagttggtac tgcgcacgcc
     8281 gtgcatcccc agcataccca gcgacaacgg atgcgctttt ggcagcatgc ccagcgccat
     8341 taaagtcatg gtggtaggca gttgcgcttt ctccgccagt tcacgcaccc gcgcaggcgc
     8401 attgatcaca ccaccaccca gataaagcac cgggcgtttg gcagcgttaa tcattgtagc
     8461 tacgtcacga atgctttctt cgctaaaggc gggtgcagcg gctttttctg ccaccgcggg
     8521 ctgagcttca atctcaaaaa ccgccgtttg tacatcctta ggaatgtcta tccacaccgg
     8581 gccagggcgg cctgattgcg caatgcggaa ggcatcgctc atgacctgcg ggagttcttc
     8641 gatatgtctg accagatagt tgtgtttggt gatggggata gagatgccgt aggtgtcgac
     8701 ttcctggaag gcgtcggtgc cgatcatcga ggcgggaacc tgaccagtga tgcaaatcag
     8761 cgggatggag tccagccgcg catcggcaat ggcggtcacc aggttagtcg cacccggtcc
     8821 gctacaggcc atacagaccg ccggtttacc gtcggtgcgc gccattccct gagcgataaa
     8881 gcccgcgccc tgttcatgac gggccagaat atggcggatt tgcgtgcttt ggcttaaggc
     8941 atcgtaaaca ggcaggatag aaccgcccgg aatgcctgtc acaatcttaa tgccctgctg
     9001 ttccaggaaa tgaacgataa attctgcgcc ggtaaagcgc ttacgcgtca atgttgtgcc
     9061 cgaacttgcc atgctccagt ccttttcttc tgggccgact ttccgggagg tgcttaaacg
     9121 aaaaaccccg cccggtttgc gccgacgggg ttttggaatc gtgtgttgtt ccagtcccta
     9181 cggcgcattg ccgacgacca ccaccacacg cacgacgacc actgcggcgg atggcgcagt
     9241 tggtagtagt tttgcgttga acatggaagt agtcattggg gaccttgttg gttttgtgtt
     9301 taacaatatt tatacaagca cagctttaca ggggagacaa tggaaaattt ttcagcaagg
     9361 gaaaattgag gggttgatca cgttttgtac tgaattgcag ataacaaaaa accccgccgg
     9421 agcgaggttt cgtcagtcgc ccgcggctgg taaccgcaaa gcacactgta ttatgtcaac
     9481 acagaaagta tacgtgttcc gcgcagaacg cgcaatttcg gcacgaattt tgacgtattt
     9541 agtgcatagt tgagtatcga tcacagtttg cgttttgtcc aaatattgct gtttatttat
     9601 acagtaaact tctataatat cactgtacgc aatgtgttat gcgggggccg catcgttacc
     9661 cggcgcacta agtcctggct gaaacgggtg gtgccgtcag cgccttaacc ccgcgtgagc
     9721 acactgtgtt atgtcaacaa gcacaaagtt tctccttgag ataccgcgtg cacaacagct
     9781 ggcaacaggc agcggaaagg tacgtcagct ggcagtgctc ctgaaccaca ggagacgcgt
     9841 atgagcctgg tggatatcgc cattcttatc ctcaaactca ttgttgcagc actgcaactg
     9901 cttgatgctg ttctgaaata cctgaagtaa ttcagattca agtcgcacca aaggggagcg
     9961 ggtaaccgct ccccttttat atttagcgtg cgggttggtg tcggatgcga tgctgacgta
    10021 tcttatccgc cctaccatct ctcccggcaa catttattgc cgcttttgtt tacatattct
    10081 gccgctaaac aactccccat tcctggcgta tatctggcta acattcatca atgtgataga
    10141 ttcctctccc gcatttatgg gaatgcgtag tgacttactc taattatttt tataaaagca
    10201 tccgtgataa tgaaaaggca tagaaacgtc aatttgttat tgatgttggt attactcgtg
    10261 gccgtcggtc agatggcgca aaccatttat attccagcta ttgccgatat ggcgcgcgat
    10321 ctcaacgtcc gtgaaggggc ggtgcagagc gtaatgggcg cttatctgct gacttacggt
    10381 gtctcacagc tgttttatgg cccgatttcc gaccgtgtgg gtcgccgacc ggtgatcctc
    10441 gtcggaatgt ccatttttat gctggcaacg ctggtcgcgg tcacgacctc cagtttgacg
    10501 gtattgattg ccgccagcgc gatgcagggg atgggcaccg gcgttggcgg cgtaatggcg
    10561 cgtactttgc cgcgagattt atatgaacgg acacagttgc gccatgctaa cagcctgtta
    10621 aacatgggga ttctcgtcag tccgttgctc gcaccgctaa tcggcggtct gctggatacg
    10681 atgtggaact ggcgcgcctg ttatctcttt ttgttggttc tttgtgctgg tgtgaccttc
    10741 agtatggccc gctggatgcc ggaaacgcgt ccggtcgatg caccgcgcac gcgcctgctt
    10801 accagttata aaacgctttt cggtaacagc ggttttaact gttatttgct gatgctgatt
    10861 ggcggtctgg ccgggattgc cgcctttgaa gcctgctccg gcgtgctgat gggcgcggtg
    10921 ttagggctga gcagtatgac ggtcagtatt ttgtttattc tgccgattcc agcagcgttt
    10981 tttggcgcat ggtttgccgg acgtcccaat aaacgcttct ccacgttaat gtggcagtcg
    11041 gttatctgct gcctgctggc tggcttgctg atgtggatcc ccgactggtt tggcgtgatg
    11101 aatgtctgga cgctgctcgt tcccgccgcg ctgttctttt tcggtgccgg gatgctgttt
    11161 cctctggcga ccagcggcgc gatggagccg ttccccttcc tggcgggcac ggctggcgcg
    11221 ctggtcggcg gtctgcaaaa cattggttcc ggcgtgctgg cgtcgctctc tgcgatgttg
    11281 ccgcaaaccg gtcagggcag cctagggttg ttgatgacct taatgggatt gttgatcgtg
    11341 ctgtgctggc taccgctggc gacgcggatg tcgcatcagg ggcagcccgt ttaagcgcac
    11401 gtcaccgcag catcgtcatc agctccatgg gagaacgatg ctgctttatc agatcacgca
    11461 tcacccgcat atgcggtgcg gagtaagaat aaaacgcctg atagcccgca caaagcacgc
    11521 tgtctggcgt ctcctgccga gcgtgcgggc acaggcgatg ccagtcgcac gcctggcagt
    11581 cacagcgcgg ttgcgggttt tcgctgccac accatttttg taaggtggcg tcaaaaagct
    11641 caatcgagat acgactgata tctgcccgca cccaacactc gaacagggca attaaaaatt
    11701 tcccccacag ctctgctgtc accgaagcgg ccgtcagatc gccccggtca tcaaattcaa
    11761 gatagggaat gagctgtacg gagcggatgc cccgctgttt gaaattgtga taaaaatcgg
    11821 caggttgcag agcatccttg tactctacaa ccagtttatg cctgtcctct tcagcatcaa
    11881 tgacctgact tcctgtcatg cgactctctc aataaatacg ataagttgat gtatatgcgt
    11941 tacagcaaac agtattgcga gagataacac cgcgagggag atcaaaaatt tgtcacggac
    12001 tacgtgaaat tgggaaaaat cgctattcgt gacatccatt aaattgcgat tacgggtgta
    12061 gtgccagagg atcagcgcca cgatggcgag aatgccaatc gaaatccaga ataacatgcc
    12121 cgcctggtgc cagttgtgtt tgatagccaa cgccatcaac gcgccgtagc ccagcatggt
    12181 acgaaaccac gccagtgatg tgcgctccgg ctgcagtcct gggtcggcaa tgcgtctggc
    12241 tttgcggcta tccggcatac aacaccagtc ccataacgat caccgccacg accatcaata
    12301 ttaagctaat gattaataag ctgttggtgt agggcaggtc ctctttcaga cgcatcgctt
    12361 tttcattgcg taaccagcgc aggtagccat acattgctaa cccgccggaa aacagacaca
    12421 acaacaacgc cagcagttca cgaatgacgg gcgtggcgaa atctggcgca agttgatcaa
    12481 gcccaacccc cgccgccaga aatcctagtg cggtacggat ccacgccaga aaagtacgct
    12541 cattagccag cgagaagcgg taatcgggcg cttctccaag gcgggaaatt ttcattgttg
    12601 ctcctttgca atttcactaa ctctctacct ttagcggttt tttttaagaa tttcgtccgc
    12661 ccgtttcctg atatgctttt gccatccgct acaaggaggt cagatgggta tcatagcgca
    12721 aaataaaatc agttcattgg gcatgctgtt cggtgccatc gctttaatga tggggattat
    12781 ccacttttca ttcggccctt tttccgcacc accgccaacg cttgaaagca tcgtggcgga
    12841 caaaacggct gaaattaaaa gaggtcttct cgccggaata aaaggcgaaa aaattaccac
    12901 cgtggagaaa aaagaggacg tagatgtcga taaaatcctc gatcaaagcg gtatcgcact
    12961 ggccattgct gcgctacttt gcgcatttgt tggcggtatg cgtaaggaaa atcgctgggg
    13021 cattcgcggc gcgctggttt ttggcggcgg aacgctggct ttccatacct tgctttttgg
    13081 tatcggcatc gtgtgcagca ttttgctgat attccttatc ttttccttcc tgaccggcgg
    13141 ttcgctggtt taagcagcct gaataatgcc cgccgggtga tcatcagcgg gcattatcgt
    13201 cagaacttct gttttttctc ttccaccttc accccttgtg tcggtaggcc ggtgtcatag
    13261 tcacgtacca ccggcgaata gccatcttgt gggcgtggac gaaatgcccc catccagcgc
    13321 ggccgtgcat ctttacgcca cggacgcagg ctccattgtt gacatcctcc acgccctgaa
    13381 ggacgtggac ccctgctacg ttcaggctgt cgcctgaatc atttcggtgg gttcctgctt
    13441 caacgagcgg cctgactgca ccatctctcc acaggcaaga acggcgtgcc ccgccgctaa
    13501 aatgttacga gcgccattta catcggcgtt cgctgtatat ccacatacct ggcatctgaa
    13561 tttactttgt gacaggcgat tttctttcgc tgtatgacca cagcacacgc aacgctggct
    13621 tgtgtacgct ggcggaacag caagcacctg accaccacgc cagagctgct tgtacgcaag
    13681 ctggcggcgc atttcatacc agccctgatc cagtatcgaa cggtttaaac ctgattttgc
    13741 ccggacattg cgccccggca ggctgaccgt acccgctgct gactttgaca tgttgctgac
    13801 cttcaaatcc tcaatgacta tcattgcgtg gtttttgctg acggtcgttg tgactttgtg
    13861 aaggtagtcc ctgcggatat ttgcgataca ggaatgcagt cgctgtattt tgcgtttctg
    13921 cttctgccag ttgttgctga atttgacctt gcggcttaac tgtcgctgaa gtctcgccag
    13981 cttcttctgg tttttctgaa aactgtttac aggctcaaag actgtgccat ctgacagcgt
    14041 ggcgagttta gccacgccag catccagccc gaccattgat gctgaagggt gaaccggagt
    14101 tgatacttca ctttctgtct gaatactgat gtaccactta ccgcaggact ggctgacagt
    14161 gacatttttc acaacacccg tgacctgacg gctgttccgg tagcgcatcc agcccagttt
    14221 cggcagaaaa atacggctgt tttcctgatc gagcttaacg ccctgcgggt agcggaatac
    14281 atcattctgt ccccgctttt tgaatcgggg aaaagcagcc cgattctgga agaagttttt
    14341 gtaagcccgc tcaaggtctt tcagtgactg ttgcaatggc tgtgacgggg catctttaag
    14401 ccattgcgtt tcagtggcat ttttccactc aaccagccag gaagccattt tcccgtaagg
    14461 gatgtattta ttaccggcct catgattctc attctgacgt gccagagcac gattgaaaac
    14521 gaaacgacat gcgcctgcga agcgcctcat ttcacgctcc tgttgaccac cgggtcttaa
    14581 ctggaattta aatgcttgta gtcgtttcac aggccaaatg atactttggt ctgtgaaaaa
    14641 agaaaccgat atccggcgtg gaaggcattg tgttttcctg atgcatgttc acctggtctt
    14701 tgtcacca
//
LOCUS       DAJVQG010000103        14394 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJVQG010000103 DAJVQG010000000
VERSION     DAJVQG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.103"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OQM83_002798"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OQM83_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCS1521013.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OQM83_002799"
     CDS             1828..3147
                     /locus_tag="OQM83_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCS1521014.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3519..4901
                     /gene="dosC"
                     /locus_tag="OQM83_002800"
     CDS             3519..4901
                     /gene="dosC"
                     /locus_tag="OQM83_002800"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCS1521015.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            4926..7325
                     /gene="dosP"
                     /locus_tag="OQM83_002801"
                     /gene_synonym="pdeO"
     CDS             4926..7325
                     /gene="dosP"
                     /locus_tag="OQM83_002801"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HCS1521016.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7584..8165
                     /gene="ddpX"
                     /locus_tag="OQM83_002802"
     CDS             7584..8165
                     /gene="ddpX"
                     /locus_tag="OQM83_002802"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HCS1521017.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8179..9729
                     /locus_tag="OQM83_002803"
     CDS             8179..9729
                     /locus_tag="OQM83_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1521018.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSGQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            9731..10753
                     /locus_tag="OQM83_002804"
     CDS             9731..10753
                     /locus_tag="OQM83_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1521019.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            10750..11646
                     /locus_tag="OQM83_002805"
     CDS             10750..11646
                     /locus_tag="OQM83_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCS1521020.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            11643..12629
                     /locus_tag="OQM83_002806"
     CDS             11643..12629
                     /locus_tag="OQM83_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1521021.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            12658..13548
                     /locus_tag="OQM83_002807"
     CDS             12658..13548
                     /locus_tag="OQM83_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1521022.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQPDVIVLDEPTSA
                     LDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQQV
                     LTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHGCE
                     VRQSLAIREDGRELRCWRAL"
     gene            complement(13604..14035)
                     /gene="osmC"
                     /locus_tag="OQM83_002808"
     CDS             complement(13604..14035)
                     /gene="osmC"
                     /locus_tag="OQM83_002808"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCS1521023.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         3571 a         3556 c         3627 g         3640 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gcttttttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg gggccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggca cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttatctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcaaagacc ttcttccctg acttctctaa agtgggcacc ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcatc cgcaacccac gtcaatgaaa
      841 tgagcaaccc agggcgcgac tatccgttgg ctatgttact gctgatggta gcggcaatct
      901 gcttaagctc tgttggtggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcacagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcta ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ctctaccgat atgtatgttg
     1501 aattactggt cgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgctcgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcatatatg gatatctgct cccgaaacga gaaattagcg
     1861 attagaagac cagcgatact ggttgcactg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggtgt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taaccccacc agccgggccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct gcaacgtctc ggtataaaca cggtcttttt ccaggttaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccat ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattta tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgca gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgag tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgca aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaagt atcgcacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ctaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact cccatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtccta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtca atctcgcacg ttgcgttttg
     3481 attccgtctg atttcccagc cttataaggg tgtgaattat ggagatgtat tttaaaagaa
     3541 tgaaagatga gtggaccgga cttgtcgaac aggcagatcc actcattcgt gctaaagccg
     3601 cggaaattgc cgttgcgcat gctcattatc tgagtatcga gttttatcga attgtccgca
     3661 tcgacccgca tgccgaagaa ttcttgagta atgaacaagt tgagcggcag ttgaaaagtg
     3721 cgatggaacg ctggattatt aacgtgcttt ctgcccaggt tgacgatgtc gaaaggctaa
     3781 tacaaatcca gcataccgtc gcggaagtgc atgcccgcat aggaattccg gtagaaattg
     3841 tcgagatggg gtttcgggtg ctgaaaaaaa tcctctatcc ggtaatcttc tcttcggatt
     3901 attccgccgc agaaaaactt caggtctatc atttctcgat taacagtatt gatatcgcga
     3961 tggaagtgat gacccgcgcg tttaccttta gtgacagtag tgcctcaaag gaagatgaaa
     4021 attatcgtat cttctcgtta ctggaaaacg ccgaagaaga aaaagaacgg caaatagcct
     4081 caatactttc atgggaaata gatattatct ataaaatcct gctggattct gatttaggca
     4141 gtagtttgcc attaagccag gctgattttg gcctgtggtt taaccataaa ggtcgacatt
     4201 attttagtgg cattgccgaa gtaggccata tctcccgtct cattcaggat ttcgacggta
     4261 ttttcaatca aaccatgcgt aacaccagga atttgaataa cagaagtctg cgggtgaaat
     4321 ttttattaca gataagaaat accgtatcgc agattattac cttgctgcgt gaattgtttg
     4381 aagaagtatc gcgccacgaa gtcggtatgg atgtactaac gaaattactt aaccgccgtt
     4441 tcctgccgac tatcttcaaa cgcgaaattg cccatgccaa ccggaccggt acaccgctgt
     4501 cagtgctgat tattgacgtt gataaattca aagagatcaa cgatacgtgg ggccataaca
     4561 ctggcgatga aattctgcgt aaagtctctc aggcctttta tgacaacgtc cacagtagtg
     4621 attatgtttt tcgctacggg ggcgatgaat ttatcattgt tttgactgaa gcttctgaaa
     4681 acgaaacgtt acgtaccgca gaacgtattc gcagtcgggt ggagaaaacc aaactgaaag
     4741 ccgcaaacgg cgaggatatt gccctctcac tttccatcgg tgccgccatg tttaatggtc
     4801 atcctgacta tgagcgcctc attcaaatag ccgatgaagc tctgtatatc gccaaaagac
     4861 gaggtagaaa ccgtgttgaa ctctggaaag ccagtcttta gatgcgccag gatgcagagg
     4921 taatcatgaa gctaaccgat gcggaaaatg ccgccgatgg catttttttc cccgcccttg
     4981 aacaaaatat gatgggcgcg gtgttaatta acgaaaatga tgaagtgatg tttttcaacc
     5041 ccgccgcaga gaagctctgg gggtacaaac gtgaagaagt cattggcaat aacattgata
     5101 tgctgattcc gcgggatttg cgtcctgcgc atcctgaata cattcgtcat aaccgtgaag
     5161 gtggtaaagc gcgcgtcgag gggatgagtc gggagctgca gctggagaaa aaagacggca
     5221 gtaaaatctg gacccgtttt gcgctatcga aagtgagcgc cgaggggaaa gtttattacc
     5281 tggcgctggt acgggatgcc agcgtagaaa tggcgcaaaa agaacagacc cgacaattga
     5341 ttattgccgt tgaccatctc gaccgaccgg tgattgtcct cgatccggaa cgccatattg
     5401 tgcagtgcaa tcgcgcattt accgaaatgt ttggttactg cattagcgaa gccagcggta
     5461 tgcagcccga tacactcctg aacattcctg aattccctgc cgataaccgc attcgtttac
     5521 aacagttgct atggaaaacc gcccgcgatc aggacgaatt tctgctgttg acacgcaccg
     5581 gtgaaaaaat ctggattaaa gcctctatca gcccggttta tgacgtgctc gcgcatctgc
     5641 agaacctggt aatgaccttc tcggatatca ccgaagaacg gcagatccgc cagcttgaag
     5701 gcaatattct cgccgccatg tgcagcagcc cgccatttca tgaaatgggg gaaatcattt
     5761 gtcgtaacat cgaatctgtt ctcaacgaat cgcatgtttc gctgttcgca ctgcgcaacg
     5821 ggatgccgat acactgggcg tcatcttcgc acggtgcaga agttcaaaat gcgcaaagct
     5881 ggtcagcgac cattcgtcag cgtgatggtg cgcctgcggg gatcctgcaa attaaaacct
     5941 cgtcaggagc agaaaccagc gcctttatcg aacgcgtggc agatatcagc cagcatatgg
     6001 ccgcgctggc gctggaacag gaaaaaagcc gtcagcatat tgaacaactc atccaatttg
     6061 atccgatgac cggtctgcca aatcgcaata acctgcacaa ttacctcgat gaccttgtcg
     6121 acaaagccgt ctctccggtg gtgtatctca tcggtgttga ccatattcag gatgtgattg
     6181 atagccttgg ctatgcgtgg gccgatcagg cattgctgga agtggtcaat cgctttcgtg
     6241 aaaaactcaa accggatcag tatctctgtc gtatcgaagg tacgcagttt gtcctcgtga
     6301 gcctggaaaa cgacgtcagt aacattaccc aaatcgccga tgagctacgg aatgtggtca
     6361 gcaagccgat aatgattgac gataaaccat tcccgctaac cttgagtatt ggcatcagct
     6421 acgacgtggg taaaaaccgc gattacctgc tctccactgc tcacaatgca atggattata
     6481 ttcgcaagaa tggcggtaac ggctggcagt tcttcagccc ggcgatgaac gaaatggtaa
     6541 aagagcgttt ggttttaggc gcagcgctga aagaagcgat tagcaataac caactgaaac
     6601 tggtttacca gccgcaaatc ttcgcagaaa cgggtgaact gtacggcatc gaagcccttg
     6661 ctcgctggca cgatccccag catggtcatg tacccccttc acggtttatt cctctcgcag
     6721 aagagattgg tgaaatcgaa aatattgggc gctgggtcat cgcggaagct tgccgtcagt
     6781 tagcagaatg gcgtagccag aatattcata tcccggcgtt atccgtgaac ttgtcggcgc
     6841 tgcactttcg cagtaatcag ctgcctaatc aggtgtctga tgcaatgcaa gcctggggta
     6901 ttgacggcca ccagctgacg gtagaaatca cggaaagcat gatgatggaa cacgataccg
     6961 aaatctttaa gcgcattcag atcctgcgcg atatgggcgt gggcttatcg gtagatgatt
     7021 ttggcacggg cttttccgga ttatcccgct tagtcagtct tccggtaacg gaaatcaaaa
     7081 ttgacaaaag ttttgtcgat cgttgtctga cggaaaaacg catccttgcc ttacttgaag
     7141 ccattaccag cattgggcaa agcctcaatt taaccgtcgt ggcggaaggc gtcgaaacca
     7201 aagagcaatt tgagatgcta cgcaagatcc actgtcgcgt tattcaggga tatttctttt
     7261 cccgccccct acccgccgag gaaattccag gctggatgag cagcgtgtta ccgctgaaaa
     7321 tctgacaaat tcctctcgcc cgcactcgcg ggttttcatt taacgtgacg ctgtcactta
     7381 aatactgtga tttcagctac gattccggga gattccttcc ttaacttccc ctcttgcacc
     7441 aaaactgcac tacgctgcac aaatgttgat caaaagttaa aaaatataac tttaagccag
     7501 cattctggcg cttatcccgg catggcatga gatctgcata agcggaaagc gcagcaattt
     7561 ttgtcttaat acaggaaccg tttatgtcgg ataccaccga actggttgat ttagccgtga
     7621 tcttccctga tctggagatc gaattgaaat acgcctgcgc tgataacatc acaggtaaag
     7681 ctatttacca gcaagcgcgt tgtctgttac acaaggatgc gattaccgcg ctggcgaaaa
     7741 gtatcagtat cgcccagctg tcaggcttac aactggtgat ttacgatgcg tatcgcccac
     7801 aacaagcaca ggcgatgttg tggcaagcct gcccagaccc gcaatatgtt gttgatgtga
     7861 cggtcggttc taatcacagc cgtggcacgg cgatcgacct gacgcttcgt gatgagcacg
     7921 ggaacatcct ggatatgggc gcagggttcg atgaaatgca cgagcgctcc catgcctatc
     7981 acccttccgt cccgcccgcc gctcagcgca atcgcctgtt gctgaatgcg ataatgactg
     8041 gtggcggttt tgtcggtatc tccagcgaat ggtggcactt cgaattaccc caggcagcga
     8101 gttaccctct gctcgctgat caattctctt gttttatatc ccctggcaca cagcacgtca
     8161 gttaatcagg aaactgtcat gaagagatcg atatcgtttc gtcccacatt gctcgcgctc
     8221 gtccttgcca caactttccc ggttgcgcac gccgccgtac cgaaagatat gctggtgatt
     8281 ggtaaggccg ccgatccaca aaccctcgac ccggcggtaa caatagataa taacgactgg
     8341 acagtgacct acccgtctta tcagcggctg gttcagtaca aaacggacgg tgataaaggc
     8401 tcaaccgacg ttgaaggcga tctggcaagt agctggaaag cgtctgacga tcaaaaagag
     8461 tggacgttca ccctgaagga caatgctaaa tttgccgatg gcacacctgt cactgccgaa
     8521 gcagtaaaac tctcttttga gcggttacta aaaatcggcc aggggccagc agaagcattt
     8581 cccaaagatt taaagattga tactcccgac gaacatacgg tgaagtttac ccttagccaa
     8641 ccattcgcac cgttcctcta cacgctagcg aatgacggtg catccattat caatccggcg
     8701 gtcttaaagg aacatgcagc ggatgatgct cgcggcttcc tcgcgcaaaa taccgccggt
     8761 tccggaccat ttatgctgaa aagcgggcaa aaaggtcagc aattagttct ggtgccaaat
     8821 ccgcattacc ccggcaataa accgaacttt aagcgagtat cggtaaaaat tatcggtgaa
     8881 agtgcctccc gtcgcctgca gctctcccgt ggcgacattg acattgccga tgcgctgccg
     8941 gtggatcaac tcaacgccct gaagcaggaa aataaagtca atgtggcaga gtatccgtca
     9001 ctgcgcgtta cctatctgta tctgaataac agcaaagcgc ctcttaatca ggcggatctg
     9061 cgtcgggcca tttcctggtc taccgattat cagggcatgg ttaacggcat tctgagtggt
     9121 aacggaaaac agatgcgcgg cccgattccg gaaggcatgt ggggctacga tgcgacggca
     9181 atgcaataca accatgacga aacgaaagcc aaagccgaat gggataaagt gacgagcaaa
     9241 cccaccagcc tgacgtttct ctactccgat aacgatccga actgggagcc tattgctctg
     9301 gcgacacaat ccagtctcaa caagctgggc atcaatgtga agctggaaaa gctggcgaac
     9361 gccaccatgc gcgacagagt gggtaaaggt gattacgaca ttgcgattgg caactggagt
     9421 ccggattttg ccgacccgta tatgtttatg aattactggt ttgagtcaga caaaaaaggt
     9481 ctgccgggta accgctcgtt ctatgaaaac agtgaggtcg ataagttact gcgcaatgcg
     9541 cttgcgacca ccgaccagac gcagcgtacc cgggactacc agcaggcaca gaaaatcgtc
     9601 attgatgacg ctgcttatgt gtacctgttc cagaaaaact accaactggc gatgaacaaa
     9661 gaggtgaaag gctttgtgtt caatcccatg ctggaacagg tcttcaatat caataccatg
     9721 agtaaataac gtgaccttct ggagtatttt acgccaacgc tgctgggggc tggtgctcgt
     9781 ggtggcgggc gtctgcgtga ttacgtttat tatctcgcat ctgatccctg gcgatccggc
     9841 gcggttactg gcgggtgacc gcgccagcga tgctatcgtg gaaaatattc gccagcaact
     9901 gggactggac cagccactgt atgtacagtt ttaccgctac ttcagcgacc tgtttcatgg
     9961 tgacctggga acatccattc gtaccgggcg tccggtgctg gaagagttgc gtatattttt
    10021 cccggcgacg ctggaactgg ctttttgtgc cctgctgctg gcactcctga ttggcatccc
    10081 gctgggcata ctctctgcag tctggcgaaa tcgctggctg gatcatctgg tgcgaataat
    10141 ggccattacc ggaatctcca cacctgcgtt ctggcttgga ctgggcgtca ttgtgctgtt
    10201 ttatggtcat ctgcaaattc ttcccggcgg cggaaggctt gatgactggc tggatccacc
    10261 aacgcacgtt accggctttt atctgctcga tgcgctgctt gaaggcaacg gtgaagtctt
    10321 cttcaatgcg ttgcaacatc tcatcttacc ggcattaacg ctggcgttcg ttcacctggg
    10381 aattgtcgct cgccagatcc gctcagcgat gctggaacaa ttgagtgaag actacattcg
    10441 taccgcccgg gccagcggct tgcccggctg gtatatcgtt ttatgttatg cgctacccaa
    10501 tgcgttgatc ccatcgatta ccgtattggg tctggcgctg ggcgatttgt tgtatggcgc
    10561 agtgctcacc gaaaccgttt ttgcctggcc cggaatgggt gcatgggtag taacatcaat
    10621 acaggcgctc gacttcccgg cagtgatggg ctttgccgtc gtggtttcat ttgcttatgt
    10681 gctggtcaac ctggtggtgg atttgctcta tttgtggatt gatccgcgta tcggacgtgg
    10741 aggtggtgaa tgatgctaag cgaggaaacg tccgccgtac gcccacaaaa acaaacgcga
    10801 tttaacggtg caaaactggt atggatgctg aaaggcagtc cgctcaccgt gaccggcgca
    10861 gtcatcattg tattaatgct attgatgatg attttttcac cgtggcttgc gacgcatgat
    10921 cccaacgcca ttgatttaac cgcccgcctt ttgccgcctt ctgcggcgca ctggtttggc
    10981 accgatgaag tgggacgcga tctgttcagc cgcgtactgg tcggcagtca gcaatcaatt
    11041 ctcgccggat tagtcgtggt tgccattgcg ggtatgattg gttcgctact cggatgtcta
    11101 tccggtgtgc ttggcggacg cgcagacgcc atcatcatgc gcatcatgga cattatgctg
    11161 tcgattcctt cgttggtact gacaatggca ctggctgccg ctctcgggcc gagtttattt
    11221 aacgccatgc tggcgattgc tattgtgcga attccctttt atgtgcgcct ggcgcggggg
    11281 caagcattag ttgtacgcca gtatacctat gttcaggcgg cgaaaacctt tggtgcgtcg
    11341 cgttggcatc tgatcagctg gcatatttta cgtaactccc tgccgccgct gatcgtgcag
    11401 gcatcgctgg atatcggtag cgcgatttta atggccgcca cgttgggatt tattggcctg
    11461 ggtgctcaac aaccgagtgc tgaatggggc gcgatggtag ctaatggtcg caactatgta
    11521 ctcgatcagt ggtggtattg cgcatttccg ggggcagcga ttttgcttac cgccgtcggg
    11581 tttaatctct ttggtgatgg tattcgcgat ctgcttgacc cgaaagcagg aggaaagcag
    11641 tcatgaccca acccgttctg gacattcaac agctgcattt gagtttcccc ggttttaacg
    11701 gttatgttca cgtgctcaac aatgtgtcct tgcagattaa ccgcggtgaa attgtcggtc
    11761 tggtgggaga atccggctca ggtaaatcag tcaccgcaat gctgattatg cgtctgctac
    11821 cgacgggcag ttattgcgta catcgggggc agatttcact gctgggagac gatgttctta
    11881 acgctcggga gaaacagctt cgtcagtggc gcggcgcacg agtggcgatg atctttcagg
    11941 aaccgatgac cgccctcaat ccgacacgtc gaataggtct tcagatgatg gacgtgatcc
    12001 gccatcatca accaataagt cgtcgggaag ccagagctaa agcgattacc ctgctggaag
    12061 agatgcaaat cccggatgcc gtggaagtta tgtcgcgcta tccgtttgag ctttcaggtg
    12121 gtatgcgcca gcgggtaatg attgcgctgg cattctcctg cgagccgcaa ttgattattg
    12181 ccgacgaacc gactacggcg ctggacgtca cggtacagtt gcaggtactg cgtttgctta
    12241 aacataaagc tcgcgccagt ggaactgcgg tactgttcat cagccatgat atggccgtgg
    12301 tgtcgcaact gtgcgatagc gtttacgtga tgtatgccgg aagcgtaatt gaaagcggcg
    12361 tgacggcaga cgttatccat catccccggc atccgtatac cattggtttg ctgcaatgcg
    12421 caccagaaca tggagtacca cgccagccat tacccgccat tccagggacg gtaccaaacc
    12481 tcacccattt gcctgacggc tgcgcttttc gcgatcgttg ctatgcggca ggtgcacagt
    12541 gtgaaaacgt cccggcgctg acagcgtgtg gtgacaacaa ccagcgctgc gcctgttggt
    12601 atccgcagca ggaggtcata agtatctgac acgttattaa cgttacgcga cgtccatatc
    12661 aatttcccgg cccgtaaaag ctggcttggt aaaactacgg aacatgttca tgcgattaat
    12721 ggtattgatt tacagatccg ccgtggtgaa accttaggga tcgtcggtga gtcaggctgc
    12781 ggcaaaagca ccctcgcaca gcttttaatg ggtatgctgc aaccgagcca cgggcaatac
    12841 atccgttcag gatcacaacg cattatgcag atggtgtttc aggacccgct ctcttcgctt
    12901 aatccgcgct tatcggtgtg gcgcatcatc acagaaccgc tctggatagc taagcgtagt
    12961 agtgaacaac agcggcgagc gttggcaaag gagttggctg tgcaggtggg tattcgtccg
    13021 gagtatctcg accgcctgcc tcatgcgttc tccggcgggc agcggcaacg catcgccatt
    13081 gccagagcac tctcttcgca gcctgacgtg attgtgcttg atgagccaac ctcggcgctg
    13141 gatatctccg tgcaggcgca gatcctcaat ttactggtaa cgctacagga aaatcacggg
    13201 ctgacttatg tgctgatttc acacaatgtc tcggtgatac gtcatatgag cgatcgggtg
    13261 gcggtgatgt atctcgggca gattgtagaa ctgggcgacg cgcagcaggt gctgacggca
    13321 cctgcacatc catacacccg attattgctg gattccctcc ccgccattga taaaccgctg
    13381 gaggaagaat gggcattatg taaaacggat ctgccgggaa accgcacgtt gccacaaggc
    13441 tgttttttct acgaacgttg ccctctggca acccacggat gtgaagtccg gcaatcatta
    13501 gcaataaggg aggacggacg tgagctccgg tgctggcggg cgctgtagag atgctaacaa
    13561 cgcatcaggc attcacgcgc ctgacgcgtc atccggcaat gctttacgat ttcaactggt
    13621 aatccagcgt aatttccgct ttcagtacct gagaaaccgg gcatcctgct tttgctttct
    13681 ggatgatgcc gtcaaaggta gaggcatcaa tacccggcac cgcaacttca ctcttcagtg
    13741 cgattttcgt aatcgcaaaa tcggcatcca ctttatccag cgacacatcg gcggtggtat
    13801 caatcgatgt tggcgtgaat cccgcttccc ccagcattaa tgaaagcgcc attgagaaac
    13861 atgcggcatg cgctgcgcca atcagttctt cagggttggt tcctttttcg ccttcaaaac
    13921 gcgtgttaaa tccatacggc tgttggttca gcacgccact ctgggtggat actgttccct
    13981 tcccgcgttt gatatcgcct tcccagtgtg cctgaccttt cttatggatt gtcattgttg
    14041 ctctcctgtg ggcttaaaaa ggaaattata gactacgtga gaaacagcac gaggataagc
    14101 aggatattcc gaataaaata cggcgaaaat attacttctg cacttaaatt aaattcccac
    14161 aatagataac cgggaatata tatctcaaga gatattgcat caatatcaat caactcacca
    14221 caataataat acatatcaat atcatggagt taaaatataa aaatcatcaa ccaggactaa
    14281 tcttaacaac gaagcggcaa atatttgcca tgctgaatgt gctttataaa tctgcgatcc
    14341 gtagcagaca ccataaatac acagacacgg agaatcacta tgtttactta ttat
//
LOCUS       DAJVQG010000104        14369 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJVQG010000104 DAJVQG010000000
VERSION     DAJVQG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14369
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.104"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..696)
                     /locus_tag="OQM83_002809"
     CDS             complement(<1..696)
                     /locus_tag="OQM83_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1521024.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAG"
     gene            952..1107
                     /gene="rseD"
                     /locus_tag="OQM83_002810"
     CDS             952..1107
                     /gene="rseD"
                     /locus_tag="OQM83_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCS1521025.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1104..1679
                     /gene="rpoE"
                     /locus_tag="OQM83_002811"
     CDS             1104..1679
                     /gene="rpoE"
                     /locus_tag="OQM83_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCS1521026.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1712..2362
                     /gene="rseA"
                     /locus_tag="OQM83_002812"
     CDS             1712..2362
                     /gene="rseA"
                     /locus_tag="OQM83_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCS1521027.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2362..3318
                     /gene="rseB"
                     /locus_tag="OQM83_002813"
     CDS             2362..3318
                     /gene="rseB"
                     /locus_tag="OQM83_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCS1521028.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3315..3794
                     /gene="rseC"
                     /locus_tag="OQM83_002814"
     CDS             3315..3794
                     /gene="rseC"
                     /locus_tag="OQM83_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCS1521029.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3992..5791
                     /gene="lepA"
                     /locus_tag="OQM83_002815"
     CDS             3992..5791
                     /gene="lepA"
                     /locus_tag="OQM83_002815"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCS1521030.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGSLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5807..6781
                     /gene="lepB"
                     /locus_tag="OQM83_002816"
     CDS             5807..6781
                     /gene="lepB"
                     /locus_tag="OQM83_002816"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCS1521031.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKLGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7054..7734
                     /gene="rnc"
                     /locus_tag="OQM83_002817"
     CDS             7054..7734
                     /gene="rnc"
                     /locus_tag="OQM83_002817"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCS1521032.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7731..8636
                     /gene="era"
                     /locus_tag="OQM83_002818"
     CDS             7731..8636
                     /gene="era"
                     /locus_tag="OQM83_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCS1521033.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8648..9376
                     /gene="recO"
                     /locus_tag="OQM83_002819"
     CDS             8648..9376
                     /gene="recO"
                     /locus_tag="OQM83_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCS1521034.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9388..10119
                     /gene="pdxJ"
                     /locus_tag="OQM83_002820"
     CDS             9388..10119
                     /gene="pdxJ"
                     /locus_tag="OQM83_002820"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCS1521035.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10119..10499
                     /locus_tag="OQM83_002821"
     CDS             10119..10499
                     /locus_tag="OQM83_002821"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCS1521036.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10721..10801
                     /gene="shoB"
                     /locus_tag="OQM83_002822"
     CDS             10721..10801
                     /gene="shoB"
                     /locus_tag="OQM83_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCS1521037.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="OQM83_002823"
     CDS             complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="OQM83_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCS1521038.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            11470..12660
                     /locus_tag="OQM83_002824"
                     /pseudo
     CDS             11470..12660
                     /locus_tag="OQM83_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(12845..14032)
                     /locus_tag="OQM83_002825"
     CDS             complement(12845..14032)
                     /locus_tag="OQM83_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCS1521039.1"
                     /translation="MLPRFADIFQQGNHWLNWLEKQPERAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLTEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNAPHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(14032..>14369)
                     /locus_tag="OQM83_002826"
     CDS             complement(14032..>14369)
                     /locus_tag="OQM83_002826"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521040.1"
                     /translation="KPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECCAD
                     LNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVH
                     WPDGVAPPF"
BASE COUNT         3621 a         3606 c         3708 g         3434 t
ORIGIN      
        1 gcccgcgcgc cacgccatag caatgccatc gccagaagaa atatccggat tggtggtgta
       61 ctgatacacc ttcgacgcac cgccggttgc cagtaccact gcttttgcgt ggcaggtttc
      121 caccgtttct ttatttcggt tccataccca cgcgcctaca acccgtcgcg tgcccggcag
      181 gccaattttg tcagagacaa tcagatcaac tgcgttgctg cgctccagca cacgaatatt
      241 cggatggttc tgcgccttgc tcaccagcgt ggattgtact tctctaccgg tggcgtcggc
      301 ggcatgaaga atacgacggt gactatgtcc accttcacgg gtcagatggt agctttcttc
      361 gccattcggt tgaacgtggg tatcaaacaa aaccccctgg tcaattaacc attgcacaca
      421 ggaacgagca ttgctggcga caaattcaac tgcatggcga tcgcaaatac cagccccggc
      481 aatcaatgtg tcttccacat gcgagtcaat gctgtcagtt tcatcaaaca cggcggcaat
      541 accgccctgg gcataaaatg ttgaaccttc cgttaccggg cctttactta gaacgatgac
      601 ctgatgctgg tcagccaggc gtagcgccag tgaaagtccg gctgcgccgc taccaataat
      661 caacacgtca catgaatgtt cagggagagt attcattttc tttgtttaat ttactaaaca
      721 ttgtttggtc agcatagcat catgttgtgc gaataaacac ctgctatttt aatatttgtt
      781 acagtggcta aacacgttga cgcagggcgg cgagaaaaag agaagttact ggctggtgga
      841 ggattaggtg gtgaaataaa aaggccgttg ggttactctt caggcagtta aatgggcatt
      901 tctacacaga taatgcgatg ttcagattct gtagacttat aatgatagat aatgatccgt
      961 ctacagcatg acaaacaaaa acagatgcgt tacggaactt tacaaaaacg agacactcta
     1021 accctttgct tgctcaaatt gcagctaatg gagtggcgtt tcgatagcgc gtggaaattt
     1081 ggtttgggga gactttacct cggatgagcg agcagttaac ggaccaggtc ctggttgaac
     1141 gggtccagaa gggagatcag aaagccttta acttactggt agtgcgctat cagcataaag
     1201 tggcgagtct ggtttcccgc tatgtgccgt cgggtgatgt tcccgatgtg gtacaagaag
     1261 cttttattaa agcctatcgt gcgctggatt cgttccgggg agatagcgct ttttatacat
     1321 ggctgtatcg gattgctgta aatacagcga aaaattacct ggttgctcag gggcgtcgtc
     1381 caccttccag tgatgtggat gccattgaag ctgaaaactt cgaaagtggc ggcgcgttga
     1441 aagaaatttc gaaccctgag aacttaatgt tgtcagaaga actgagacag atagttttcc
     1501 gaactattga gtccctcccg gaagatttgc gcatggcaat aaccttgcgg gagctggatg
     1561 gcctgagcta tgaagagata gccgctatca tggattgtcc ggtaggtacg gtgcgttcac
     1621 gtatcttccg agcgagggaa gctattgata acaaagttca accgcttatc aggcgttgac
     1681 gatagcggga tactggataa gggtattagg catgcagaaa gaacaacttt ccgctttaat
     1741 ggatggcgaa acgctggata gtgagctgct taacgaactg gctcataacc cagaaatgca
     1801 gaaaacctgg gaaagctatc acttaatccg tgattcaatg cggggtgata ctcccgaggt
     1861 gctccatttc gatatctctt cacgcgtgat ggccgccatt gaagaagagc cagtacgtca
     1921 accggcgaca ttgatcccgg aagcccagcc tgcgccgcat caatggcaca aaatgccatt
     1981 ctggcagaaa gtacgtccgt gggcggcaca gcttacccaa atgggcgtag cagcatgcgt
     2041 atcgcttgca gttatcgttg gcgtccagca ctataatgga caatctgaaa cgtcccagca
     2101 gcccgaaacg ccggtattta atacactgcc gatgatgggt aaagccagcc cggtaagcct
     2161 gggagtacct tctgaagcga ccgcaaacaa tggtcaacag cagcaggtac aggagcagcg
     2221 tcgtcgcatt aatgcaatgt tgcaggatta cgaactgcaa cgccgactcc actctgaaca
     2281 gcttcagttt gagcaggcgc aaacccagca agccgctgta caggtgccag gacttcaaac
     2341 tttaggaacg caatcgcagt aatgaagcaa ctttggtttg ccatgtcatt agtgacaggt
     2401 agcctgttat tctctgctaa cgcctcggcc actcccgcgt ccggggcgtt attacagcag
     2461 atgaacctgg ccagtcagtc actgaattac gagctgtcat tcatcagcat caataaacag
     2521 ggtgttgagt ctctgcgtta tcgacatgca cgcctcgata accgtcctct tgcacaattg
     2581 ttgcaaatgg atggcccgcg ccgggaagtg gtacagcgcg gcaatgaaat cagctatttt
     2641 gaaccgggac ttgaaccgtt cacgcttaat ggcgattaca ttgttgattc tctgccatcg
     2701 cttatctata ccgatttcaa acgcctttct ccttactacg actttatctc cgtcgggcgc
     2761 acgcgtattg ctgatcgtct ttgcgaagtc attcgcgtgg ttgcccgaga tggtacacgc
     2821 tacagctaca tcgtgtggat ggacactgaa tcgaaattac cgatgagggt tgatctcctt
     2881 gatcgcgatg gtgaaacgct ggaacaattt cgcgtgattt cttttaacgt caatcaggat
     2941 atcagcagca gtatgcagac gctggcgaag gcaaatttgc cgccgttgct ttctgttcct
     3001 gtaggtgaaa aagctaaatt cagctggacg ccaacctggt tgccacaggg ttttagcgaa
     3061 gtttccagta gtcgacgtcc gctaccgacg atggacaaca tgcctatcga atcacgtctc
     3121 tattccgacg gattattcag cttctcggta aacgttaacc gcgctacgcc atcgagcacc
     3181 gatcagatgt tgcgcaccgg acgcagaacc gtcagtacaa gcgtacgtga taacgccgaa
     3241 atcaccattg tcggtgaact gccgccgcaa acggcgaaac gcattgccga gaatattaag
     3301 ttcggggcag cgcaatgatc aaagagtggg ctaccgtcgt ctcctggcaa aacgggcagg
     3361 cgctggtcag ttgtgatgtt aaagcctcat gcagcagctg cgcttcacgc gccggttgcg
     3421 gtagccgcgt gttgaataaa cttggcccgc aaaccacgca taccattgtc gtaccctgtg
     3481 atgaaccgtt agtgccgggg caaaaagtgg aattggggat cgccgaaggc agcctgctta
     3541 gctccgcatt actggtttat atgtcgccgc tggtgggatt attccttatc gcttcgctat
     3601 ttcagctact ctttgcttct gacgttgcag cattatgtgg tgcgatcctt ggcggtattg
     3661 gcgggtttct gattgcgcgc ggttactcgc gtaagtttgc tgcccgggca gaatggcaac
     3721 cgatcatatt aagcgtggca ttgccgcctg accttgtgcg atttgaaaca tcttcggaag
     3781 acgcgagcca gtgatttcac tggctttgtt gcattttgca taacaaatgc gaggacgttt
     3841 ccagatacca caatccatac ggtatgaaca tttcctcgcc tcaatgttgt agtgtagaat
     3901 gcggcgtttc tattaataca gacgttaagc tcagaacagc gacttctaaa agcctggtta
     3961 accgggcatt aaggcacaat aatcatactt tatgaagaat atacgtaact tttcgatcat
     4021 agctcacatt gaccacggta aatcgacgct gtctgaccgt attatccaga tctgcggtgg
     4081 cctgtctgac cgtgaaatgg aggcgcaggt tctcgattcc atggatcttg agcgtgagcg
     4141 tggcattacc atcaaagcgc aaagcgtgac gctggactac aaagcgtctg acggcgaaac
     4201 ctatcagctt aactttatcg acaccccggg ccacgtagac ttctcctatg aagtttcccg
     4261 ttcgctggct gcctgtgaag gtgcattgct ggtggtcgac gccgggcagg gcgtggaagc
     4321 gcaaaccctg gcaaactgct acaccgccat ggaaatggat ctcgaagtgg tgccagtact
     4381 gaacaagatt gacctgccgg cagccgatcc tgaacgcgtg gcggaagaaa ttgaagatat
     4441 cgtcggcatc gacgccactg acgcggtgcg ctgttcagcg aaaaccggcg ttggcgtgca
     4501 ggacgttctc gaacgtctgg tgcgcgacat tccgccgccg gaaggcgatc cggaaggctc
     4561 gttgcaggca ctaattatcg actcatggtt cgacaactac ctgggcgttg tttcacttat
     4621 ccgtattaaa aacggcaccc tgcgtaaggg cgacaaagtg aaagtcatga gtaccgggca
     4681 gacctataac gccgaccgtc tgggcatctt cacgccgaaa caggttgacc gcactgaact
     4741 gaaatgtggc gaagtaggct ggctggtatg tgcgattaaa gatatccatg gcgctccagt
     4801 cggcgatacc ttaacgctgg cgcgtaatcc ggcagaaaag gcgctgcctg gctttaagaa
     4861 agtcaaaccg caggtatacg ccggtctgtt cccggtaagt tccgacgact atgaagcctt
     4921 ccgtgacgcg ctgggtaaac tcagcctgaa tgatgcctca ctgttctatg agccggaaag
     4981 ctccagcgcg ctgggctttg gtttccgctg cggcttcctt ggcctgctgc acatggagat
     5041 catccaggaa cgtctggaac gtgaatacga tctggatctg atcaccactg cgccgaccgt
     5101 agtgtatgaa gttgaaacca cgtcaaggga agttatctac gtcgacagcc catccaagct
     5161 gcctgcggta aataacattt acgaactgcg cgagccgatt gcagagtgtc acatgctgct
     5221 gccgcaggca tatctcggca acgttattac gctgtgcgta gaaaaacgcg gcgtgcagac
     5281 caatatggtt taccacggta atcaggtggc gctgacgtac gagatcccga tggcggaagt
     5341 ggtgctcgac ttcttcgatc gcctgaaatc tacctcgcgt ggttatgcgt ctctggatta
     5401 caacttcaaa cgcttccagg cgtccgacat ggtacgtgta gacgtattaa tcaacggtga
     5461 acgtgttgat gcgttggcgc tgatcaccca ccgtgataat tcgcaaaacc gcggtcgcga
     5521 gttggtggag aagatgaaag atctgatccc acgccagcag tttgatatcg ccattcaggc
     5581 agcgattggt acgcacatta ttgcgcgatc caccgtgaaa cagctgcgta aaaacgtact
     5641 ggctaaatgt tatggcggcg atatcagccg taagaaaaag ctgctgcaga agcagaaaga
     5701 aggtaagaaa cgcatgaagc agatcggtaa cgtcgagctg ccgcaggaag cgttcctcgc
     5761 cattctgcac gtcggcaaag acaacaaata acccttagga gttggcatgg cgaatatgtt
     5821 tgccctgatt ctggtgattg ccacactggt gacgggcatt ttatggtgcg tggataaatt
     5881 ctttttcgca cctaaacggc gggaacgtca ggcagcggcg caggcggctg ccggtgactc
     5941 actggataaa gcaacgttga aaaaggttgc accgaagctt ggctggctgg aaaccggagc
     6001 ttctgttttt ccggtgctgg ctatcgtatt gattgtacgt tcgtttattt atgaaccgtt
     6061 ccagatcccg tcaggttcga tgatgccgac tctgttaatc ggtgatttta ttctggtaga
     6121 gaagtttgct tatggcatta aagatcctat ctaccagaaa acgctgatcg aaaccggtca
     6181 tccgaaacgc ggcgatatcg tggtctttaa atatccggaa gatccaaagc ttgattacat
     6241 caagcgcgcg gtgggtttac cgggcgataa agtcacttac gatccggtct caaaagagct
     6301 gacgattcaa ccgggatgca gttccggcca ggcgtgtgaa aacgcgctgc cggtcaccta
     6361 ctcaaacgtg gaaccgagcg atttcgttca gaccttctca cgccgtaatg gtggggaagc
     6421 gaccagcgga ttctttgaag tgccgaaaaa cgaaaccaaa gaaaatggaa ttcgtctttc
     6481 cgagcgtaaa gagacactgg gtgatgtgac gcaccgaatt ctgacagtgc cgattgcgca
     6541 ggaccaggtg gggatgtatt accagcagcc agggcaacaa ctggcaacct ggattgttcc
     6601 gccgggacaa tacttcatga tgggcgacaa ccgcgacaac agcgcggaca gccgttactg
     6661 gggctttgtg cctgaagcga atctggtcgg tcgggcaacg gctatctgga tgagtttcga
     6721 taagcaagaa ggcgaatggc cgacaggtgt gcgcttaagt cgcattggcg gcatccatta
     6781 atagcccatc ttcgttcacg ttgtcgccgt tatggcgaca acgtgaatta tttatgagat
     6841 aaatctcccg tggctaacga catcccccgt cgttgtgtat agaatattcc cccgaagttt
     6901 aaggttggca cctccaggtt gccacggcac acgaaacagc gttggtcccc atataccggt
     6961 aaactgaaac tgcagcgaag cagttagcag agccatgtat atcaggtctg tttcgtgtgc
     7021 tgaattgttg acgcatttat ttattggtat cgcatgaacc ccatcgtaat taatcggctt
     7081 caacggaagc tgggctacac ttttaatcat caggaactgt tgcagcaggc attaactcat
     7141 cgtagtgcca gcagcaaaca taacgagcgt ttagaatttt taggcgactc tattctgagc
     7201 tacgttatcg ccaatgcgct ttatcaccgt ttccctcgtg tggatgaagg cgatatgagc
     7261 cggatgcgcg ccacgctggt ccgtggcaat acgctggcgg aactggcgcg cgaatttgag
     7321 ttaggcgagt gcttacgttt agggccaggt gaacttaaaa gcggtggatt tcgtcgtgag
     7381 tcaattctcg ccgataccgt cgaagcatta attggtggcg tattcctcga cagtgatatt
     7441 caaaccgtcg agaaattaat cctcaactgg tatcaaactc gtttggacga aattagccca
     7501 ggcgataaac aaaaagatcc gaaaacgcgc ttgcaagaat atttgcaggg tcgccatctg
     7561 ccgctgccga cttatctggt agtccaggta cgtggcgaag cgcacgatca ggaatttact
     7621 atccactgcc aggtcagcgg cctgagtgaa ccggtggttg gcacaggttc aagccgtcgt
     7681 aaggctgagc aggctgccgc cgaacaggcg ttgaaaaaac tggagctgga atgagcatcg
     7741 ataaaagtta ctgcggattt attgccatcg tcggacgtcc gaacgttggc aaatccacat
     7801 tgttgaacaa actgctgggg cagaagatct ccatcacctc ccgcaaagcg caaacaactc
     7861 gtcaccgcat tgtggggatc catactgaag gcgcgtatca ggcgatctac gttgataccc
     7921 ctggcctgca tatggaagaa aaacgcgcta ttaaccgcct gatgaacaaa gcggcgagca
     7981 gctctattgg cgacgttgag ctggtgattt ttgtcgttga aggcacccgc tggacgccgg
     8041 acgacgaaat ggtgctcaac aaactgcgcg atggcaaagc gccggtgatc ctcgcggtga
     8101 acaaagtgga taacgtgcag gaaaaagccg atctgctgcc gcacttacag ttcctggcaa
     8161 gtcagatgaa cttcctcgat atcgtgccga tctctgctga aaccgggctg aatgtcgaca
     8221 ctatcgcagc aatcgtgcgt aagcatctgc ctgaagcgac ccatcacttc ccggaagatt
     8281 acatcaccga tcgctcacag cgttttatgg cgtctgaaat catccgcgaa aaactgatgc
     8341 gtttcctcgg cgctgaactg ccgtactccg tgaccgtgga gatcgaacgt ttcgtctcta
     8401 acgaacgcgg tggttatgac atcaatggtt tgattctcgt tgagcgtgaa gggcagaaga
     8461 agatggttat tggcaacaaa ggggccaaga tcaaaaccat cgggattgaa gcgcgtaaag
     8521 acatgcagga aatgttcgaa gcgcctgtcc accttgaact gtgggtaaaa gtgaaatccg
     8581 gttgggccga cgacgaacgc gcactgcgca gtctcggtta cgttgacgat ctttaagagt
     8641 cactccgatg gaaggctggc agcgcgcatt tgtcctgcat agtcgcccgt ggagcgaaac
     8701 cagcctgatg ctggacgtct tcacggagga atcgggtcgc gtgcgtctgg ttgccaaagg
     8761 cgcacgctct aaacgctcta ctctgaaagg tgcattacag cctttcaccc ctctcttgct
     8821 acgtttcggc gggcgtggcg aagtcaaaac gctgcgcagt gctgaagctg tctcgctggc
     8881 gctgccatta agcggtatca cgctttacag cggtctgtac atcaacgaac ttctctcccg
     8941 cgtactggaa tacgagacgc gcttctctga actctttttc gattacttgc actgcattca
     9001 gtctcttgca ggtgccactg gtacgccaga acccgcgctg cgccgctttg aactggcact
     9061 gctcgggcat ctgggctatg gcgtcaattt tacccattgc gcgggtagcg gcgagccggt
     9121 agatgacacc atgacgtatc gttatcgcga agaaaaaggg tttatcgcaa gcgtcgttat
     9181 cgacaataaa acgttcaccg gaaggcagtt aaaagcgtta aacgcgcggg aatttcctga
     9241 cgcagacaca ctacgcgccg cgaaacgctt tacccgcatg gcgcttaagc cgtatcttgg
     9301 cggtaaacca ttaaagagca gggaactgtt ccggcagttt atgcctaagc gaacggtgaa
     9361 aacacattat gaatgatgag gattgtcatg gctgaattac tgttaggcgt caacattgac
     9421 catattgcaa cgctgcgtaa cgcacgcggt actgcttacc cggatccggt gcaggcggcg
     9481 tttattgccg agcaggcggg agcggacggc attaccgtgc atttacgtga agatcgccgc
     9541 cacattactg accgcgatgt gcgcatcctg cggcagacgc tggatacccg aatgaatctg
     9601 gagatggcag tgaccgaaga gatgctggcg atcgccgttg agacgaagcc acatttttgc
     9661 tgcctggtac cggaaaagcg tcaggaagta acaaccgaag gcggcctgga cgttgcaggg
     9721 cagcgtgaaa aaatgcgcga tgcctgcaaa cgtctgacag atgccgggat tcagatttct
     9781 ctctttatcg acgccgatga agagcagatc aaagctgcgg cagaggttgg cgcaccgttt
     9841 atcgagatcc acaccggttg ctatgctgat gccaaaactg acgccgaaca ggcgcaagag
     9901 ctggtgcgta tcgccaaagc cgcgaccttt gccgctagtc tcggtctgaa agttaacgcc
     9961 ggacacggtc tgacctatca caacgtgaaa gccattgccg ccatccctga gatgcatgaa
    10021 ctgaatatcg gtcatgccat tattggccgt gcagtgatga ccggactgaa agatgcggtg
    10081 gcagaaatga agcgtctgat gctggaagcg cgtggctaat ggcaatatta ggtttaggca
    10141 cggatatcgt agagatcgct cgcatagaag cagtgatcgc ccgatccggt gagcgcctgg
    10201 cacgccgcgt attaagcgat aacgaatggg caatctggaa aacgcaccac cagccggtgc
    10261 gttttctggc gaagcgtttt gctgtgaaag aagccgcagc aaaagcgttt ggcaccggga
    10321 tccgcaatgg tctggcgttt aatcaatttg aagtattcaa tgatgagctc ggcaaaccac
    10381 ggctacggct atggggtgag gcattaaaac tggcggaaaa gctcggcgtt gtaaatatgc
    10441 atgtaacgct ggcagatgaa cgccactatg cttgtgccac ggtaattatt gaaagttaaa
    10501 ataataccgt tctctttaaa aagtaaaacc cccgaactgg taaggaacgg gggttttact
    10561 ttttaacttt aattttgtgc agcatgcacc ctcaacaaca gcgattatgg atcagtgtac
    10621 ttatctttta caagcaactt gcaatcttta gcataaaaac tcgagccttt acgaagaaag
    10681 caatactgaa cggctgcatt ccttgtttaa ggaaaaacga atgactgatt gccgatacct
    10741 gattaaacgg gtcatcaaaa tcatcattgc tgttttacag ctgatccttc tgttcttata
    10801 acacaaggaa acgtacttaa ggtgcgtccg gtgaaccaat cggacgcgcc tttaataact
    10861 ataaataagt gtcttggcag atactatata aattaactta gtgaatgatt atgctaatgt
    10921 catcaattaa ataaatataa tggcgttaag gcttcccagt aatataatta atactctact
    10981 tccagagtag aatattaaat tttatccgcg tggtgcatca gcacaaattt atcccacaac
    11041 tgttcttctg tctcgacatg cgccggatct ttcacaatag tattggggat cgggcacacc
    11101 ttctggcagg ttggtgtctc gtagtgccct acgcattcgg tacacttatc gctgttaatc
    11161 tcgtagatat gatctcccat tgaaatcgcc tcattcgggc attcgggttc acacatatca
    11221 caattaatac agcgtttagt aattagtaaa gacatttcaa tggattaccg ttaaatcatt
    11281 ttaaaatcag taagttgtat cgagtttgta tgctttactg tcattaactt actgtatgtt
    11341 gatccagtgt atttaacctt gataaactca gtccagcaac acaaaaccgc aacacattgc
    11401 attttgtccc gtagaaaaga cttgtatgtg tgagcttgtt ttctgcgcct acgcagataa
    11461 ggattgagaa tgccgcgcac tgtaacacat aatccggata gccccaataa tgacgatgtt
    11521 ttagccgcat ctgaaaaatg ggacgcctgt aaacccccct ataccagcgc acacatgaaa
    11581 atctgtgttg ctgccgccaa aatcatcctc gctgcttccg gcgtggctcg ccgttccaaa
    11641 tacgaaaaag agaactatct ccgtatcgat ttcagcaaag ccggtaaggt tacattttac
    11701 gccgagtttc caaaaaagat gggcctcaaa ggtaaaaagc tcggcgagtg gccggagctc
    11761 gctatccagc tggcgcgcga aaaagcacta ggtatggctg acggtggact gcgggcagag
    11821 tccgtacatg cagcgctgga aatgtaccgg gatgacctca aagccaaagt agcccggcag
    11881 aagctgagcc cggacagttt cacaacctac ggggtgcgta tcgaccggat taaagcaacg
    11941 ttcggcgagc gtgaggtgtt cagcgacgta acatacaatc ggctggtgga agtgctggac
    12001 gagtggatcg ccactcgctc gaacaataac gccctggagt tgtttgccga gctccgtcgg
    12061 ttctggaagt tctgcgcacc tactctttgc aacggccgca atgttgccgc cagtctgcca
    12121 gatgattatg tttcctcccg cgtacagaaa cctaccccca cacggctttt taccgatatt
    12181 gaatcaatcg cccgactctg gctcaatgtt gctgcctgca cctctgtaca ccagaagaat
    12241 gctgttcgct tcatgatcat cactggtgtt cgtccgatta atgtccataa cctgcgctgg
    12301 gactacgttt acgaggaggc tggtgaaatt gtttatccgg aaggggttat cggcatgcga
    12361 ggggctatga aaacacaaaa ggctttccgc ctgccgataa cgcctgagat ccggcggatt
    12421 atcgacgagc agaaagcctg gcgtgattca gttcctgagt gcaacaggga ttatgtattt
    12481 ttgcagccac gtgatccaat gcagccattt tcaaaacgat cactggataa gctggtgaaa
    12541 acatacagcc cggacggggc tgtaaaagga ataaaacatg atgggactgt taaagggaaa
    12601 gacggtgcat ttaatacgat gtgccgtaaa ttccttaaga gcaatgttat tgccttgatc
    12661 gataggaatt aaaaccccaa aaagattaaa aaaacaccac aaaacggatg tttcttcaac
    12721 accacttttg ctcatatgaa cggaaccgac gattaaactg gatggctctg attgattcag
    12781 ggtatgaatg gcggtttttt gctccgtttc cctcaaaatg gacgcaactt tccctctgcg
    12841 gctctcagcc gcaccaccgc atccgggcca gcggctcatg catcaggacc tgctctgcca
    12901 gacggtagcc ccgcttcagc cccgtaaaac gcatctgact cccgcacagc acgcacttca
    12961 gcgggtcaac cttcagtaac ctctgataca tccctctcca ggtgatttgc atcgccgttt
    13021 ttctcactgt ctccgttatg atgtacacca cttcttccag taaccgccgt ttcgccggac
    13081 tcaaaaaacc gtagtacctc accatacgga accccttatc cgccacatgc caggagaacc
    13141 tttccatgaa ctcatctcca ctcatcaaca ggtattcttc ccgttttgtt cggtgactgt
    13201 tgtaacgcag accgatttca tcctgaccgg cataatgctc cagacgactc atcggcaccg
    13261 gtggcttttt caggtaagag ccaaagtaca ccgccacatg gggggcatta tccatcaccc
    13321 gggatacgtt gacattccag ccacggcggt aatgcgtgtc caggaagcga ttccattccc
    13381 gtttactgct tccttctgct gccagcgcat ccggcatcac caggtcaggg tatttccgtg
    13441 acagcaaccg tgttatccgg tagcgccaca tgctcatcac cttacgggcg taaaaatgaa
    13501 gatttttcca ggtgtggccc gacgtcacac caccggcagt tgtcgataaa tggatatgcg
    13561 gatgccactg ctggtcacgc ccccatgtgt ggatcaccgt gaatatcccc ggctccacat
    13621 ctgcctgatg gcagatttcc agtatcacat ccgctgcaat gcggctcatc tctgtcagta
    13681 accaccggtt gtggaacacc agggaccagt actggcaggg aagtgtgaac acaatatgct
    13741 gccacgggca gtcggggacc aggctcagca gatactgtat ccactgtgcg ccagccttca
    13801 ccccgcagtg cgggcaggag cggcttttac accggaagca gacctttttt gtatggcaac
    13861 agtccggtga tgaacagcac cactgtgtat accccatcag tgtggtcccg cacgccatga
    13921 ttttggtcac cgactcaatc accaccggac gtactgccct ttccggctgc ttctccagcc
    13981 agttaagcca gtggtttccc tgctgaaaga tatcggcaaa acggggaagc atcagaaggg
    14041 cggggcgact ccgtccggcc agtgaaccgt gccacactcc gggcagtaca taccgccggc
    14101 gctgataccg gaaagaatgg tcgcaaattc ccgctccgtg cagcgggcga tttccggata
    14161 cccttcgtca tcaacacgta caaaccagaa gaccagcttt ttgtttcccg catccacaaa
    14221 gaacggaata ttcaggtctg cgcagcattc aacggcatcg tcaaaactat caaagcgcag
    14281 aacttctgcg tcttcttcgt caaaaaaatc atcttcgtga agcttcacga catagcgggg
    14341 aagtttgctt ctttgagagg cgggtttac
//
LOCUS       DAJVQG010000105        14306 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJVQG010000105 DAJVQG010000000
VERSION     DAJVQG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14306
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.105"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            19..1245
                     /gene="rtcB"
                     /locus_tag="OQM83_002827"
     CDS             19..1245
                     /gene="rtcB"
                     /locus_tag="OQM83_002827"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCS1521041.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            1249..2265
                     /gene="rtcA"
                     /locus_tag="OQM83_002828"
     CDS             1249..2265
                     /gene="rtcA"
                     /locus_tag="OQM83_002828"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCS1521042.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2308..5013)
                     /gene="malT"
                     /locus_tag="OQM83_002829"
     CDS             complement(2308..5013)
                     /gene="malT"
                     /locus_tag="OQM83_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HCS1521043.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIHTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5624..8017
                     /gene="malP"
                     /locus_tag="OQM83_002830"
     CDS             5624..8017
                     /gene="malP"
                     /locus_tag="OQM83_002830"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCS1521044.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8027..10111
                     /gene="malQ"
                     /locus_tag="OQM83_002831"
     CDS             8027..10111
                     /gene="malQ"
                     /locus_tag="OQM83_002831"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCS1521045.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="OQM83_002832"
     CDS             complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="OQM83_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCS1521046.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKRIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="OQM83_002833"
     CDS             complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="OQM83_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCS1521047.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="OQM83_002834"
     CDS             complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="OQM83_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCS1521048.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13188..13958
                     /gene="bioH"
                     /locus_tag="OQM83_002835"
     CDS             13188..13958
                     /gene="bioH"
                     /locus_tag="OQM83_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCS1521049.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(13987..>14306)
                     /locus_tag="OQM83_002836"
     CDS             complement(13987..>14306)
                     /locus_tag="OQM83_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCS1521050.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         3442 a         3568 c         3996 g         3300 t
ORIGIN      
        1 cgaaaagaga aaaacaaaat gaattacgaa ttactgacca ctgaaaatgc cccggtaaaa
       61 atgtggacca aaggcgtgcc ggtagaagcc gatgcgcgtc agcaacttat taatacggcg
      121 aagatgccgt ttattttcaa acatattgcg gtaatgcctg atgtacacct gggtaaaggt
      181 tccaccattg gtagcgtgat cccgaccaaa ggggcgatta ttccggcggc ggtgggcgtg
      241 gatattggct gtggaatgaa cgcgctgcgt accgcgttaa cggcggcaga cctgcctgaa
      301 aacctggcgg agctgcgtca agcgattgaa acggccgtac cacacgggcg tactaccggg
      361 cgttgtaaac gtgataaagg cgcatgggaa aacccgcctt ttaacgtcga tgcgaaatgg
      421 gctgagctgg aggccggtta tcagtggtta acgcaaaaat atccgcgttt ccttaacact
      481 aacaactata aacacctggg aacgctggga acgggtaacc actttattga aatctgcctt
      541 gatgagtcgg agcaggtgtg gattatgctg cactccggtt cacgcggaat tggtaacgcc
      601 atcgggactt actttatcga tctggcgcaa aaagagatgc aggaccaact tgagacgttg
      661 ccgtcgcgtg atctggcgta ctttatggaa ggtacggaat actttgatga ttacctaaga
      721 gccgtggcct gggcgcagct tttcgccagc cttaaccgcg atgcgatgat ggaaaacgtg
      781 gtaacggcat tgcagagcat tacgcagaaa acggtcagac agccacaaac gctggcgatg
      841 gaagagatca actgtcacca caactatgtg caaaaagaac agcactttgg tgaagagatc
      901 tacgtgacgc gtaaaggcgc ggtgtttgcg cgtgctggtc aatatggaat tattccaggg
      961 tcgatgggag caaaaagctt tatcgtccgt gggctgggaa atgaagaatc tttctgttcg
     1021 tgcagccacg gtgccgggcg ggtaatgagc cgtactaaag cgaaaaaact gttcagcgtg
     1081 gaagatcaaa ttcgtgccac cgcgcatgtg gaatgccgta aagatgccga agtgatcgac
     1141 gaaatcccga tggcgtataa agatattgat gcggtgatgg cggcacaaag cgatctggtg
     1201 gaagttatct ataccctgcg tcaggtggtg tgcgtaaaag gataaatgat gaaaaggatg
     1261 attgcgctgg atggcgcaca gggcgaaggt ggcgggcaga tcctgcgctc ggcgctgagc
     1321 ctgtcgatga taaccggcca gccatttacc atcaccagca ttcgtgccgg gcgggcgaaa
     1381 ccggggctgt tgcgccagca tctgaccgcg gtaaaagcgg ctgcggaaat ttgtagggca
     1441 acggtggaag gcgcggagct ggggtcgcag cgtctggtct tccggcttgg caccgtgcgc
     1501 ggcggcgagt accgctttgc tatcggtagt gccggaagct gtacgctggt gctgcaaacg
     1561 gtgctgcccg cgctgtggtt tgccgatgga ccttcgcgtg ttgaagtgag cggaggcacc
     1621 gataacccgt cggccccgcc tgcggatttt atccgccggg tgctggagcc gctgctggcg
     1681 aaaataggaa ttcatcagca aaccacgctg ttacgtcacg gtttttatcc tgccggaggc
     1741 ggcgtggtgg caacggaagt ctcgcccgtg gcatcgttta acaccttgca acttggcgag
     1801 cgcgggaaca ttgtgcagat gcgtggagaa gttctattag ctggtgtgcc gcgccatgtt
     1861 gctgagcgtg aaatcgctac actggcgggg agtttttccc tgcatgaaca gaatattcat
     1921 aacctgccgc gcgaccaggg gccgggtaat accgtctcgc tggaagtcga aagtgaaaat
     1981 atcactgaac gctttttcgt cgtcggtgaa aagcgcgtca gtgccgaggt ggtagccgcg
     2041 cagttagtga aagaggtgaa acgctacctg gcaagcccgg cggtagtggg ggaatatctc
     2101 gccgaccagc tggtgctacc gatggcgctg gcgggcgcgg gagaatttac ggtcgcccat
     2161 ccttcatgcc atctgctgac caatatcgcc gtggtggagc gtttcttgcc ggtgcggttt
     2221 ggtttgatag aaacagatgg cgtaacgcgg gtgagtattg aatgatgtaa agatgtaaac
     2281 cggatccaat gcattatccg gctaaactca caccccatat cccatcatct tcagcagttt
     2341 ctgcgcgtgt tgtaccgcgg cctgacggtg ggcgacgccg agtttttggt acagattgcg
     2401 gatatgcgtc ttgatggtgg ttgccgccac ttccagctcg cccgcaattt gctcattgct
     2461 gtaaccggag tagatcagcc ccaggacctg ccattcacgt tgggtcaacg ggctggtgtg
     2521 gatcagttct ggcacttccg gatgattcag cagacgttca acgaaattct catcgaaatg
     2581 ggcgaattta tgccgatgat gttgattgat ttctcgcaga atacgctgcg cgcgatgctg
     2641 ttccagttcc ggcagcgtat tgagttgaat cagctggcgc agttgttgcg ccatcgcttc
     2701 gccttcgatg acaaaatgac tgataaatcc ggtgcgattc gccagtttta atgcgtccag
     2761 caacacgcgc tgggcgtcac ttttacgtcc ggcctgccag tacagttgat taagcagcaa
     2821 caggttacgg ttgagatcgc tcatcaaccg cagactgcgg gcattttcat tgagttcttc
     2881 gagaacaatt tccgcaggtt caaactcgcc cagcaagatt tgtgcgcggg caatgttgcg
     2941 ccattgacct tgcaggaagt ggttgttcgc aaactccggt ttagccgtat gacgcaacca
     3001 gttggcagcg gcggctttat cgccggtcat ttgccagtaa atcacccgga ctttgttggc
     3061 gttagagatc cagtcgctgt gatatttgcc attccccagc aggttttcca gacggttcag
     3121 ctggctacgg gcgttatcta aatcaccacg ggccagcgag cattgaatca acattgccag
     3181 gcactgaagc tgttgctgtg gctgataaga cgacaagact tcaatcccgc tacgcgccga
     3241 cgcttcggct tcatccagcc gtgcccaggc ccataacagt tgtgctcgga tgcgcaccag
     3301 aaactcatgc atcggcagct gttccagatg ctgctcgttg atcagctgga acgttttttc
     3361 ctgtgtttcc cacgcggttt gcaggaaccc ttgggcaaac agaatttcac tttgctggat
     3421 taaactccat aaggcgtagt gccagacgtc gtgctggcgt gccatctgtt cggtttgctg
     3481 cattagcgcc agtgagcggg tcaattcgcc tttgcagtgc agcacttcac ccagcaccga
     3541 ggttgctaca atgcggctat agaaccagcc cggtggcagc tcttccagtg ccagttttgc
     3601 cagccgttcc gcttcatccg gattaccatc gttaatcgcc acctgggcgc gcagagcgtt
     3661 aaattctgcg tgcatggtgc cttctctgat gtccttgatt tcatgttcag cacgggctag
     3721 cagggtgtta acttcgccat agcgatgttg gctttgcatt agccacgcct gcaataacac
     3781 taactgcgga ttttccagca aactgtccca cggcagggcc ttaagcgact cttccagcag
     3841 cgacagttcg ctatggttga acaaactcca ggcgtgatta agcagaatat cgcgcagcat
     3901 cagcgcatcg cctgccgcca gcgcatgatg aatcgcttcg ctgggaaatc cctgggccat
     3961 ccagctttct gcggcggcac ggtggatttc cggcagctcc gccgccagtt cccactggca
     4021 gcgctggcgc aggaagttgc caaacagcgg gtgatagcag aaccactcgc cggtatcatc
     4081 catccgctgt aaaaacagcc cctgacgctc aatctcttcg agacgcattt gcccgttttc
     4141 ttcgccagtc acacgggtga tgagggcatc gttcattgag cgcaaaatgg cgcttttcaa
     4201 cagaaaatgg cgcgttgcga gatcgacgtt atccaaaacc tcatcgacca gataatccga
     4261 aagatggctg gcattgattc ccgccaggcg gcgtgccgac ttatgggctg agtgggtatt
     4321 ctgccgggcg gagagggcga ttagctgtag tgccgtcgcc caaccggaaa cgtcatcgca
     4381 aatccaactg ctttctgcgg cttcaatcgg cgatgacaga cggcaatcaa aaaactgctt
     4441 cgcttcctga tgggtaaatg ccagttgctg actgccaatt tccagcagtt ggtcacgaac
     4501 acgcagattg gcaatgccca gttgcggaag gttgcgtgac aacaccacca gggtgagatt
     4561 ttctggttga tggcgaataa agaagcgcat tgactcgtgg atcactggat tagtgatcag
     4621 atgatagtca tcgatgacca gataaagtgg gctatgccat tccgccagct caatgaaaag
     4681 ctgggcgaag agtgacgtca ggctggcata ttgccgtttt tgcgccatcg tctcacatat
     4741 cgcacagtga ccgttggttg cctgctgcac ggcggcaatg agatagctgg cgaaacgctc
     4801 ttgctggtta tcaccttcat ccagcgagta ccagccgata tcgtttttgc ctgccgccca
     4861 ctgggaaatg agggtggtct ttccgtagcc cgcaggactc gtgatcagcg ccagccggaa
     4921 gttgttcgcg ccggaaagtt tagccagcag gcgctcacga accacggtat ggtcgagtcg
     4981 aaccggacga cttagttttg acggaatcag catagttaat cacttcactg tggaaaatga
     5041 ggaaatatta tttttttgcg cttcgtaatt aatggttata aggtcggcca gaaacctttc
     5101 taatgcaagc gatgacgttt ttttatgtgt ctgaatttgc actgtgtcac aattccaaat
     5161 ctttattaac aactcaccta aaacgacgct gatccagcgt gaatactggt ttcccttatg
     5221 ttcatcagat tcatttaagc aagggtttct tcttcattcc tgatgaaagt gccatctaaa
     5281 aagatgatct taataaatct attaagaatg agatggagca cattggatat tttacttgtg
     5341 aaactgtttc actcctttac ttaatttata gagttacctt ccgctttttg aaaatacgca
     5401 acggccattt tttgcactta gatacagatt ttctgcgctg tattgcattg atttgctgct
     5461 aatcctgtgg tttgcactag ctttaagtgg ttgagatcac atttccttgc tcatccccgc
     5521 aactcctccc tgcctaatcc cccgcaggat gaggaaggtc aacatcgagc ctggcaaact
     5581 agcgataacg ttgtgttgaa aatctaagaa aagtggaact cctatgtcac aacctatttt
     5641 taacgataag caatttcagg aagcgctttc acgtcagtgg cagcgttatg gcttaaattc
     5701 tgcggctgaa atgactcctc gccagtggtg gctagcagtg agtgaagcac tggccgaaat
     5761 gctgcgtgct cagccattcg ccaagccggt ggcgaatcag cgacatgtta actacatctc
     5821 aatggagttt ttgattggtc gcctgacggg caacaacctg ttgaatctcg gctggtatca
     5881 ggatgtacag gattcgttga aggcttatga catcaatctg acggacctgc tggaagaaga
     5941 gatcgacccg gcgctgggta acggtggtct gggacgtctg gcggcgtgct tcctcgactc
     6001 aatggcaact gtcggtcagt ctgcgacggg ttacggtctg aactatcaat atggtttgtt
     6061 ccgccagtct tttgtcgatg gcaaacaggt tgaagcgccg gatgactggc atcgcagtaa
     6121 ctacccgtgg ttccgccaca acgaagcact ggatgtgcag gtagggattg gcggtaaagt
     6181 gacgaaagac ggacgctggg agccggagtt taccattacc ggtcaagcgt gggatctccc
     6241 cgttgtcggc tatcgtaatg gcgtggcgca gccgctgcgt ctgtggcagg cgacgcacgc
     6301 gcatccgttt gatctgacta aatttaacga cggtgatttc ctgcgtgccg aacagcaggg
     6361 catcaatgcg gaaaaactga ccaaagttct ctatccaaac gacaaccata ctgccggtaa
     6421 aaagctgcgc ttgatgcagc agtacttcca gtgtgcctgt tcggtagcgg atattttgcg
     6481 tcgccatcat ctggcggggc gtaaactgca cgaactggcg gattacgaag ttattcagct
     6541 gaacgatacc cacccaacta tcgcgattcc agaactgctg cgcgtgctga tcgatgagca
     6601 ccagatgagc tgggatgacg cttgggccat taccagcaaa actttcgctt acaccaacca
     6661 taccctgatg ccagaagcgc tggaacgctg ggatgtgaaa ctggtgaaag gcttactgcc
     6721 gcgccatatg cagattatta acgaaattaa tactcgcttt aaaacgctgg tggagaaaac
     6781 ctggccgggc gatgaaaaag tgtgggccaa actggcggtg gtgcacgata aacaagtgca
     6841 tatggcgaac ctgtgtgtgg ttggcggttt cgcggtgaac ggtgttgcgg cgctgcactc
     6901 ggatctggtg gtgaaagatc tgttcccgga atatcaccag ctatggccga acaaattcca
     6961 taacgtcacc aacggtatta ccccacgtcg ctggatcaaa cagtgcaacc ctgcactggc
     7021 ggctctgttg gataaatcac tgcaaaaaga gtgggctaac gatctcgatc agctgatcaa
     7081 tctggaaaaa ttcgctgatg atgcgaaatt ccgtcagcaa tatcgcgaga tcaagcaggc
     7141 gaataaagtc cgtctggcgg agtttgtgaa agttcgtacc ggtattgaga tcaatccaca
     7201 ggcgattttc gatattcaga tcaaacgtct gcatgagtac aaacgccagc acctgaatct
     7261 gctgcatatt ctggcgttgt acaaagaaat tcgtgaaaac ccgcaggctg atcgcgtacc
     7321 gcgcgtcttc ctcttcggcg cgaaagcggc accgggctac tacctggcga agaatattat
     7381 ctttgcgatc aacaaagtgg ctgacgtgat caacaacgat ccactggttg gcgataagtt
     7441 gaaggtggtg ttcctgccgg attattgcgt ttcggcggcg gaaaaactga tcccggcggc
     7501 ggatatctcc gaacaaattt cgactgcagg taaagaagct tccggtaccg gcaatatgaa
     7561 actggcgctc aatggtgcgc ttactgtcgg tacgctggat ggtgcgaacg ttgaaatcgc
     7621 cgagaaagtc ggtgaagaaa atatctttat ttttggtcat accgtggaac aagtgaaggc
     7681 aattctggcc aaaggctacg acccggtgaa atggcggaag aaagataagg tgctggacgc
     7741 agtattgaaa gagctggaaa gcggtaaata cagcgacggc gataagcatg ccttcgacca
     7801 gatgctgcac agtatcggca aacaaggcgg cgatccgtat ctggtgatgg cggatttcgc
     7861 agcctatgtt gaggcacaaa agcaggtgga tgtgctgtac cgcgaccagg aggcctggac
     7921 tcgcgcggcg atcctcaata ccgcccgctg cggtatgttt agctcggatc gctctattcg
     7981 cgattatcag gctcgtatct ggcaggcaaa acgctaagga agctcgatgg aaagcaaacg
     8041 tctggataat gccgcgctgg cggcggggat tagccccaat tacatcaatg cccacggtaa
     8101 accgcagtcg attagcgccg aaaccaaacg gcgtttgctt gacgcgatgc atcaacgtac
     8161 cgccacgaaa gtggcggtaa cgccagtccc gaatgtcatg gtttatacca gcggcaaaaa
     8221 aatgccgatg gtggtggagg gcagcggcga atatagctgg ctgctgacca ccgaagaagg
     8281 aacacagtac aaaggccatg taacgggggg caaagcgttc aatctaccga cgaagctgcc
     8341 ggaaggttat cacacgctga cactcaccca ggacgaccag cgcgcgcatt gccgggtgat
     8401 tgtcgccccg aaacgctgtt acgaaccgca ggcgttgctg aataaacaaa agctgtgggg
     8461 tgcctgcgtt cagctttata cgctgcgatc ggaaaaaaac tggggtattg gggattttgg
     8521 cgatctcaaa gcgatgctgg tggatgtggc aaaacgtggc gggtcgttca tcggcctgaa
     8581 cccgattcat gcgctctatc cggcaaatcc ggagagtgcc agcccataca gcccgtcttc
     8641 tcgccgttgg ctgaatgtga tttatatcga cgttaaagcc gtcgaagatt tccatcttag
     8701 cgaagaggct caggcctggt ggcagttgcc gaccacgcaa cagacgctgc aacaagcgcg
     8761 ggatgccgac tgggtcgatt actccacggt taccgcccta aaaatgacag cattacgaat
     8821 ggcgtggaaa ggtttcgcgc aacgtgatga tgagcagatg gccgcgtttc gccagtttgt
     8881 tgcagatcaa ggcgacagcc tgttctggca ggcagccttt gatgcgctac atgcccagca
     8941 agtgaaagag gacgaaatgc gctggggctg gcctgcatgg ccagagatgt atcagaacgt
     9001 ggattcacca gaagtgcgtc agttctgcga agaacatcgt aatgacgtcg atttttatct
     9061 ctggttgcag tggctggctt acagccagtt tgccgcctgc tgggagataa gccagggcta
     9121 tgaaatgccg attggcttgt atcgtgatct ggcggttggc gtagcggaag gtggggcgga
     9181 aacctggtgt gaccgtgaac tatattgcct gaaagcgtcg gttggcgcgc cgccggatat
     9241 cctcggcccg ttggggcaga actggggatt accgccaatg gacccgcata tcatcaccgc
     9301 gcgtgcctat gaaccgttta tcgagctgtt gcgtgccaat atgcaaaact gcggcgcatt
     9361 acgaattgac catgtgatgt cgatgctgcg tttgtggtgg ataccgtatg gcgagacggc
     9421 agatcagggc gcgtatgttc actatccggt ggatgatctg ctctcgattc tggcactcga
     9481 aagtaaacgt catcgctgta tggtgattgg tgaagatctc ggtaccgtac cggtagagat
     9541 tgtcggtaag ctgcgcagca gcggtgtgta ctcctacaaa gtgctctatt tcgaaaacga
     9601 ccacgagaag acgttccgtg caccgaaagc gtatccggag cagtcgatgg cggttgcggc
     9661 gacacatgac ctgcctacgc tacgcggtta ctgggagagc ggggatctaa cgctgggcaa
     9721 aaccctgggg ctgtatccgg atgaagtggt actgcgcggt ctgtatcagg atcgcgaact
     9781 ggcgaagcaa gggctgctgg atgcactgca taaatatggt tgtctgccaa aacgtgccgg
     9841 gcataaggca tcgttgatgt cgatgacgcc gacgctgaac cgtggtttgc agcgctacat
     9901 tgccgacagt aacagtgctc tgttaggact acagccggaa gactggctgg atatggccga
     9961 accggtgaat attcctggca ccagttacca gtataaaaac tggcgacgca agctttccgc
    10021 aacgcttgag tcgatgtttg ccgatgatgg cgtgaacaag ttgctgaagg atttggacag
    10081 acggcgcaga gctgcagcga agaagaagta gagtgcggtt gatgccggat gcgatgcatc
    10141 cggcagtcag tgttatcaaa tcaccatatt cagcagcaga cagcctacca gaccgcacac
    10201 cgagataatg gtttccagca tcgaccagga tttgatggtc tcgccgatag tcaggttaaa
    10261 gtactctttg aacagccaga agcccggatc gttcacatga gagaaaatca cactaccgga
    10321 acccaccgca ataaccatca gctctgggct aacacccgtc gttgcaatca gcggtgccgc
    10381 gataccaccc gcagtgattg ccgcaacggt tgcggaaccc agcgcgatac gcagtacggc
    10441 agcaatcgac caggccatca gcagcggaga aatgttggtt tcgtgcatca tggaagcaat
    10501 gtatttgtcc acgccgctgt ctaccagcac ctgcttgaac gcaccgccac caccgatgat
    10561 caacagcatc atcgcaatga ttttgatgga agaaaccagc gtgtcgttaa tctgatccat
    10621 tgaacgacca cggttcagac caaaggtgaa catcgcaatc agcacggcaa tcagcgttgc
    10681 cattaccggg tcaccgagga actccgctac cggcaggaaa gcgtgacctt tcggcaggat
    10741 catttcggca atcgcacgca tcgccatcag cacaaccggc accagagaag tccagacgct
    10801 gacgccaaag ctcggcatct cttcttcgct gaaggttttc gcgctgtaga gaccttccgg
    10861 gattggctta tcgatacctt tcaacacgcg agcgtaaacc ggaccggcga gaatcacggt
    10921 cgggattgcc agaatagtac cgtacagcag ggttttaccc atatcggcat tgaaaatggt
    10981 ggcaatcgca gtcgggcccg gatgcggtgg caggaagccg tgggtcacag acagtgcagc
    11041 cgccattggt acaccaacat acagcagtgg aatattcgcg gaagccgcga tggtaaacac
    11101 cagcggcagc atcagcacga agcccacttc atagaacagg gcaaaaccaa cggtaaaacc
    11161 ggttaacacc accgcccact ggatgcgctt tttaccaaat ttggcaatca gcgtggtggc
    11221 gatacgttgt gcgccaccgc agtctgccag cattttgccc agcattgcgc caaaacccat
    11281 gatcagggca aggctaccga gcgtcccgcc gacaccgact ttgatggagt caataacttt
    11341 atccagcggc attccttgca ttaatccaac agcaagcgcc acgaggacga gagcgatgaa
    11401 gccgttcatt ttgaagcgga tcatcaggag caacaacaag attacaccga tagcaacaat
    11461 gactaatggc atgatttacc tggcctttca tttgttatgg gtaacgtcaa ttttctgacg
    11521 acaaactcta attatcccaa tcgggaacag agatattgcg gcaccacgac tgatacccac
    11581 taaaactaat tattgtagtc agatgtcagg agtatgtttg gtacccatgt gaatgatacg
    11641 ggtaacattt ggcgtttgag aatcaccaga gcggggtaaa tttaaattat gagaggttgg
    11701 tcatattatc gcggggaaac gaagggaggg atttgccaaa gcaatgctgt gccaacgtct
    11761 ggcacatgtt caacgtaggc ccgaaatgac gctttagcgt cgcatcgggc aatctacaaa
    11821 agaggggata acttagtagt aggagtgttc gccgcgctgg tgttcggtga gatcgcgcac
    11881 acctttcagc tccgggaatt cgttcagcag ctgcttctcg atcccttctt tcagcgtcac
    11941 atcgaccatg gaacaaccgt tacagccgcc gccaaattgc agaatggcgt aaccgtcttc
    12001 ggtgatttcc atcagcgaaa cgcgaccacc gtgaccagca agctgtgggt tgatctgcga
    12061 ctgcagcata tactccacgc gctccatcag cggtgcatcg tctgccactt tacgcatttt
    12121 ggcgttcggg gctttcagcg ttaactgaga acccaactgg tcggtaacaa agtcgatctc
    12181 tgcatcttcc aggtatggtg cgcttaactc atcaacatac gcggtcagca ggtcaaattt
    12241 cagggcagtg tcggtggctt ccacggcatc cggcggacaa taagaaacac cacattcagc
    12301 gttaggcgtg ccagggttaa tcacaaatac gcggatttgt gtcccttctt cctgatttgc
    12361 cagcagtttg gcaaagtgcg cttgtgcagc atcggaaata cggatcatag taatggccta
    12421 atagttgact attttagttg gttataatac gcccatcatc gaggctctac aaggttcgac
    12481 aaaggcacca gacctggaca gtcgccgcac cattgcgtaa aagcaactgc gcaatctctg
    12541 cgacggtact tccggtggta acgacatcat ccacaatcac catatggcga ccttgcacgg
    12601 gcaattcaag acgaaaggca tttttcaggt tgcgcttgcg cagccgggca ctgagaaaat
    12661 gctgggtcgc agtggcccgt gtacgtgtga cggcttcgct atcccattgg cagtgcaacc
    12721 agcgtgataa cggctgacac agcaaatcgc tctgattaaa tccccgacgc cagtgacgcc
    12781 gctgccataa cggaacgctg atgatgcgat ccggcaattg caacccggtg gtgcgacgag
    12841 cgtgtaagac ttccaatagt aacagacgtg acagggcgct ggcgatttca ctgcgccggg
    12901 aaaatttaag ctggtggatg agcagactta acggcggcgc atagtcggca accgtgacca
    12961 gtctttgcca gggcggcggt ttttgcaggc agcgaccgca gggaagatgg gagtgtgtgg
    13021 cgggtaatcc acattgtggg cataacgttt tatctgtgcg ggtggcgcgt gaacagaccg
    13081 aacaaatccc ccaatgacct aacgccagtg gcattcggca tagccagcat aatcccggta
    13141 ctgttagcat atgttcatcc ttgtaagtca aaagagaaca atagcggatg aataacattt
    13201 ggtggcagac caaaggtcag gggaatgttc atcttgtgct gctgcacgga tggggactga
    13261 atgccgaagt gtggcgttgc attgacgagg aacttagctc gcattttacg ctgcaccttg
    13321 ttgatctgcc cggcttcggg cgcagccagg gatttggtgc gctgtcactt gctgatatgg
    13381 ccgaagccgt gctgcaacag gcacctgata aagccatttg gttaggctgg agtctgggcg
    13441 ggctggtggc aagccagatt gcgttaaccc atcccgagcg tgttcaggcg ctggtcaccg
    13501 tggcgtcgtc accttgtttt agtgctcgtg acgattggcc ggggataaaa ccggacgtgc
    13561 tggcgggatt tcagcagcaa ctcagtgatg attttcagcg tacagtggag cggttcctgg
    13621 cgttacaaac catggggact gaaacggcgc gccaggatgc gcgggcgttg aagaaaaccg
    13681 ttctggcgtt accgatgccg gaggttgacg tgcttaatgg cgggctggaa atcctgaaaa
    13741 cggttgatct ccgtcagccg ctgcaaaacg tgcccatgcc gtttttgcga ttgtatggct
    13801 atctcgatgg tctggtgccg cgcaaagtgg tgccgatgct ggataaactc tggcctcgca
    13861 gcaaatcata tatcttcgcc aaagcggccc atgcgccgtt tatttcgcat ccggtcgagt
    13921 tttgtcacct gctggtggcg ttgaagcaga gggtgtaggc aacttttgaa atggcgagac
    13981 agaaatttac tggctcgccg cagccaactc ttcttctgac accccggtaa agcgcatgat
    14041 gtccgcaaga gggactccgg attcaagcat tattttggct atatgcaggg ctttggattg
    14101 ttccccctcc tgccgcaatc tttccgcaat tgtcattaaa ctctccttgt gtttcggtga
    14161 acgttcggca acgccgtcga taaaatcgtt aaaacgtacc gcgtcgccgg tttgcagtat
    14221 gtaattaaac agccctttga tttgtctgtc attagcgtat ccactactta ataagcaggc
    14281 catttgctct accagcccca tcaggt
//
LOCUS       DAJVQG010000106        14159 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJVQG010000106 DAJVQG010000000
VERSION     DAJVQG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14159
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.106"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..228)
                     /locus_tag="OQM83_002837"
     CDS             complement(<1..228)
                     /locus_tag="OQM83_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HCS1521051.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            385..561
                     /locus_tag="OQM83_002838"
     CDS             385..561
                     /locus_tag="OQM83_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HCS1521052.1"
                     /translation="MLNLPIRKLFPTRIASSYGMNFEFMPELKWSFGYPGAIIFMILA
                     GLTPYLYFKRKNWL"
     gene            1187..1486
                     /locus_tag="OQM83_002839"
     CDS             1187..1486
                     /locus_tag="OQM83_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521053.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     ILNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(1533..2420)
                     /gene="rarD"
                     /locus_tag="OQM83_002840"
     CDS             complement(1533..2420)
                     /gene="rarD"
                     /locus_tag="OQM83_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCS1521054.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(2472..2939)
                     /locus_tag="OQM83_002841"
     CDS             complement(2472..2939)
                     /locus_tag="OQM83_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCS1521055.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            3104..3973
                     /gene="pldA"
                     /locus_tag="OQM83_002842"
     CDS             3104..3973
                     /gene="pldA"
                     /locus_tag="OQM83_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCS1521056.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            4106..5935
                     /gene="recQ"
                     /locus_tag="OQM83_002843"
     CDS             4106..5935
                     /gene="recQ"
                     /locus_tag="OQM83_002843"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCS1521057.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            5999..6616
                     /gene="rhtC"
                     /locus_tag="OQM83_002844"
     CDS             5999..6616
                     /gene="rhtC"
                     /locus_tag="OQM83_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCS1521058.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(6678..7298)
                     /gene="rhtB"
                     /locus_tag="OQM83_002845"
     CDS             complement(6678..7298)
                     /gene="rhtB"
                     /locus_tag="OQM83_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCS1521059.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            7409..8431
                     /gene="pldB"
                     /locus_tag="OQM83_002846"
     CDS             7409..8431
                     /gene="pldB"
                     /locus_tag="OQM83_002846"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCS1521060.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8439..9239
                     /gene="yigL"
                     /locus_tag="OQM83_002847"
     CDS             8439..9239
                     /gene="yigL"
                     /locus_tag="OQM83_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCS1521061.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            9315..10214
                     /gene="bioP"
                     /locus_tag="OQM83_002848"
     CDS             9315..10214
                     /gene="bioP"
                     /locus_tag="OQM83_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCS1521062.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(10102..11055)
                     /gene="metR"
                     /locus_tag="OQM83_002849"
     CDS             complement(10102..11055)
                     /gene="metR"
                     /locus_tag="OQM83_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCS1521063.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            11291..13552
                     /gene="metE"
                     /locus_tag="OQM83_002850"
     CDS             11291..13552
                     /gene="metE"
                     /locus_tag="OQM83_002850"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCS1521064.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(13837..14082)
                     /locus_tag="OQM83_002851"
     CDS             complement(13837..14082)
                     /locus_tag="OQM83_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCS1521065.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3447 a         3424 c         3690 g         3598 t
ORIGIN      
        1 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
       61 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      121 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      181 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      241 tggttatatt taagctcaag atcaattcga gaacaaatgc tagggtaaaa aaatatttaa
      301 aaaagaagta cacttcacat tttttcatta catctctgaa gagaacctcg ttaatgattg
      361 gtcttgctca ttgttaataa caaaatgttg aatttaccga ttaggaaact ttttcctacg
      421 cggattgcct ccagctatgg catgaacttt gagtttatgc cagaactgaa gtggagtttc
      481 ggctacccag gcgcgattat ctttatgatc cttgcgggcc tgacaccgta tctgtacttt
      541 aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt gtgctgacag
      601 cgacatttta gccgagggaa tcgcagataa cataattgtt gtgaataata tccatgccac
      661 gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag agataataga
      721 aaaaagtgtc aaacccaatg aaatcaatac aaagtagatt gccttacatc cagtatctct
      781 tattcttttt gctccaatat tgatatttac ataggtatta ttaataacca gaataaaact
      841 caaagccagg ttgatatcat tcaaagttat taatattgac catgatgtta atgtatagat
      901 tatgaactga aagcgtgata aacgctcaag cctggatggg tagaatatat ttctgatgga
      961 tgatttgaac atttttacat tgttttatat atataattgt tttgaatata tattacctga
     1021 attatgtggt ttgcatatat atatgcaata tagcagtctt tgcattgata actatacagt
     1081 agtaatttag agtgtgtaag tttttattga tggggtatat tttaattaaa tataattgaa
     1141 atgtattttt attctataaa caatatcttg ggtgaatgga ttctatatgg gaaatggtaa
     1201 cttttggcag ttgttcttgg gtaaagaacc ttacagcaaa aaatctgatt ttgttaaggc
     1261 atggttattg tggtgggcta ttaccatcct tgttctattt attttaagcc tgactacgat
     1321 attaaattta agaagtatag gtatagggtt aagttttggg ttttgtcaaa tccttggtag
     1381 atatacgatg tctctaggat ggagtaagta ctggggagtt ttgggggtat ttcctgtaac
     1441 aaatattgtt ctttttctta tcttaatttt tttcaaaaaa agataaataa gtcagtgggt
     1501 aggctcttta aacggcctac ccatgttaat tcttaacttt ttctacgctg cgtataaatc
     1561 gcatccatca caaaaattgc cagcgccacc caaataaagg cgaaagtcac catcttatcg
     1621 gcacccggtt tttcaccata aaacgtcaca gccagcagga acatcagcgt cgggccaatg
     1681 tactggaaaa agcctaacgt tgagagacgc aagcgcgtgg cagcggcggt aaaacacaac
     1741 agcggtacgg tagtgacaat accggcggcg atcagcagta aattcagcga catcgggttt
     1801 tgccccatat ggctggttga gctgtcggca atagcaaaca ggtaaattgc cgccacgggc
     1861 agcagccaca tggtttcgat taacatgccg gtttgcgctt caacggcaat cttcttgcgt
     1921 accagaccgt agaaggcaaa actaaatgcc agtcccagcg cgataatagg tagcgaacca
     1981 aaagtccaca gctggactaa cacgccacat atcgccagaa tcaccgccag ccattgcatc
     2041 cggcggaatc gctcgccgag gaaaatcatc cccagcacaa tgttcaccag cgggttaata
     2101 aagtaaccaa ggctcgcttc cagcatatgg tgattgttca ccgcccagat aaacagtagc
     2161 cagttgccac caatcagcac ggcagagact gccagcataa aaattttctg tggcgtctga
     2221 atcagcgttt ttaaatagga ccactggcgg caaatgctca tcagcaccac cataaagaaa
     2281 aacgaccaga tcacgcgatg cgtcaggatt tcatcggcgg gcacgtagta aatcaacttg
     2341 aagtacgctg gcgctatacc ccaaataaaa taagcggcaa gagcgagtaa tacgccctgc
     2401 cgcgtttgtt ttgcatccat cgggaatact catttttaat tggtaacagc agtttacctg
     2461 cttttatgtc ttcaacctac catataggtg gcggtggcac tggcaatata aagctgttct
     2521 tcattgtgta attcaacgcg ggcgacggcg actttattgc ctgcacgcaa caggctactg
     2581 gtagcagtaa aacgctcgcc cctgcctggg cgcagataat caacgcgaag atcaatggtc
     2641 cccatccgcg atagccgctg gcgtagttca tcttcactga tggtttcgtg gcgggttaag
     2701 gtacttccca cgcacaccag accggcggcg acatccagcg ccgacgcaat gaccccgccg
     2761 tgcaaaatgc tttgcgccca gttgcccacc atcattggct gatttttaaa ggccagctgt
     2821 gcgaactctt tttcgtaacg ctccagttcc atccccaatg cgcggttaaa tggcatgtga
     2881 taaacaaaca tctcacccac taatttcagg gcttgttcag cggtcagtac ggcagacata
     2941 tcatccttac acttcattgg ttaatgaaat gttgatttta tgcttctttg ttgttggttt
     3001 ctactttagg aagggataac taacggctat ggagttaagt atgtaaaata gcccgcagaa
     3061 aaatattcac cttatcaata attcgttacg gagaacacga ccgatgcgga ctctgcaggg
     3121 ctggttgttg ccggtgttta tgttgcctat ggcagtatat gcacaagagg caacggtgaa
     3181 agaggtgcat gacgcgccag cggtgcgtgg cagtattatc gccaatatgc tgcaggagca
     3241 tgacaatccg ttcacgctct atccttatga caccaactac ctcatttaca cccaaaccag
     3301 cgatctgaat aaagaagcga ttgccagtta cgactgggcg gaaaatgcgc gtaaggatga
     3361 agtaaagttt cagttgagcc tggcatttcc gctgtggcgt gggattttag gcccgaactc
     3421 ggtgttgggt gcgtcttata cgcaaaaatc ctggtggcaa ctgtccaata gcgaagagtc
     3481 ttcaccgttt cgtgaaacca actacgaacc gcaattgttc ctcggttttg ccaccgatta
     3541 ctgttttgca ggttggacgc tgcgcgatgt ggagatgggg tataaccacg actctaacgg
     3601 gcgttccgac ccgacctccc gcagctggaa ccgcctttat actcgcctga tggcagaaaa
     3661 cggtaactgg ctggtagaag tgaagccgtg gtatgtggtg ggtaatactg acgataaccc
     3721 ggatatcacc aaatatatgg gttactacca gcttaaaatc ggctatcacc tcggtgatgc
     3781 ggtgttgagt gcgaaaggac agtacaactg gaataccggt tacggcggcg cggagttagg
     3841 cttaagttac ccgatcacca aacatgtgcg cctttatact caggtttaca gcggctatgg
     3901 cgaatcgctc atcgactata acttcaacca gacccgtgtc ggtgtaggag ttatgctaaa
     3961 cgacttgttt tgatgaacgg ttgagtggtt ggcaaatctg gaacccagca tccaggatta
     4021 ccctctcaga gactaaaagc attgcagttt ctcgcgcagg cgctgaaaat agcgcctgtt
     4081 tttatttcag gcaatcgggg ggaatgtggc gcaggcggaa gtgttgaatc tggagtccgg
     4141 agctaaacag gttttacaag aaacctttgg ctaccaacag tttcgccccg gccaggaaga
     4201 aattatcgac actgtgcttt ccggtcgcga ttgcctggtc gtcatgccca ccggtggcgg
     4261 aaaatccctt tgctatcaaa ttcctgcctt attgctaaac ggccttaccg tggttgtttc
     4321 accgctgatt tcgttgatga aagatcaggt ggatcaactg caagccaacg gcgtggcggc
     4381 ggcgtgcctt aactcgacgc aaacccgcga gcagcaactt gaagtgatga caggctgccg
     4441 caccgggcaa attcgtctgc tttatatcgc cccggaacgc ctgatgctgg ataactttct
     4501 tgagcatctg gcgcactgga atccggtgtt attagccgtt gatgaagcgc actgtatctc
     4561 ccaatggggc cacgatttcc gcccggaata tgccgcgctt ggtcagttac gccagcgttt
     4621 cccgaccctg ccgtttatgg cgctgactgc cacagccgac gacaccacgc gccaggatat
     4681 cgtgcgcttg ctggggctga acgatccgct gattcaaatc agcagttttg accgtccgaa
     4741 tattcgctac atgctgatgg agaaattcaa accgctcgat cagttgatgc gctacgtgca
     4801 ggaacagcgc ggtaagtccg gcattatcta ctgcaacagc cgggcgaaag tagaagacac
     4861 cgctgcgcgc ctgcaaagca agggtattag cgcggcggcc tatcatgccg ggctggaaaa
     4921 taatgtccgc gccgacgtgc aggagaaatt ccagcgcgat gacctgcaaa ttgtggtggc
     4981 gacggtggcg ttcggcatgg gcatcaataa accaaacgtt cgcttcgtgg tccactttga
     5041 tattccgcgc aatatcgaat cctattatca ggaaaccggt cgcgccgggc gtgatggcct
     5101 gcctgcggaa gcgatgctgt tttacgatcc ggctgatatg gcgtggctgc gccgttgtct
     5161 ggaagagaag ccgcaggggc agttgcagga tattgagcgc cacaaactca atgcgatggg
     5221 cgcgtttgcc gaagcgcaaa cttgccgtcg tctggtattg ctgaactatt ttggcgaagg
     5281 gcgtcaggag ccgtgcggga actgcgatat ctgcctcgat ccgccgaaac agtacgacgg
     5341 ttcaaccgat gctcagattg ccctttccac cattggtcgt gtgaatcagc ggtttgggat
     5401 gggttatgtg gtggaagtga ttcgtggtgc taataaccag cgtatccgcg actatggtca
     5461 tgacaaactg aaagtctatg gcatgggccg tgataaaagc catgaacatt gggtgagcgt
     5521 gatccgccag ctgattcacc tcggcctggt gacgcaaaat attgcccagc attctgccct
     5581 acaactgaca gaggccgcgc gcccggtgct gcgcggcgaa tcctctttgc aacttgccgt
     5641 gccgcgtatc gtggcgctca aaccgaaagc gatgcagaaa tcgttcggcg gcaactatga
     5701 tcgtaaactg ttcgccaaat tacgcaaact gcgtaaatcg attgccgatg aaagtaatgt
     5761 cccgccgtac gtggtgttta acgacgcaac cttgattgag atggctgaac agatgccgat
     5821 caccgccagc gaaatgctca gcgttaacgg cgttgggatg cgcaagctgg aacgctttgg
     5881 taaaccgttt atggcgctga ttcgtgcgca tgtcgatggc gacgacgaag agtagtcagc
     5941 agcataaaaa agtgccagga tgaagactcc gtaaacgttt cccccgcgag tcaaatgtat
     6001 gttgatgtta tttctcaccg tcgccatggt gcacattgtg gcgcttatga gccccggtcc
     6061 cgatttcttt tttgtctctc agaccgctgt cagtcgttcc cgtaaagaag cgatgatggg
     6121 cgtgctgggc attacctgcg gtgtaatggt ttgggctggg attgcgttgc ttggcctgca
     6181 tttgattatc gaaaaaatgg cctggctgca tacgcttatg gttggcggtg gcctgtatct
     6241 ctgctggatg ggttaccaga tgctgcgcgg cgcgctgaaa aaagaggcgg tttctgcacc
     6301 tgcgccacag gtcgagctgg cgaaaagtgg gcgcagtttc ctgaaaggtt tactgaccaa
     6361 tctcgctaat ccgaaagcga ttatctactt tggctcggtg ttctcattgt ttgtcggtga
     6421 taacgttggc actaccgcgc gctggggcat ttttgcgctg atcattgtcg aaacgctggc
     6481 gtggtttatc gtcgttgcca gcctgtttgc cctgccgcaa atgcgccgtg gttatcaacg
     6541 tctggcgaag tggattgatg gttttgccgg ggcgttattt gccggatttg gcattcattt
     6601 gattatttcg cggtgatgcc agacgcgtct tcagagtaag tcggataagg cgtttacgcc
     6661 gcatccgaca ttatttttca cgcatgcctc gccgatgcta acagcgctcc caccagcata
     6721 aacaacgagc cgaaaatctt attcagcgcc ttcatctgct ttggtccttt aatccataga
     6781 gcaatccgtt gagcaagggt ggcgtaaccg atcatcacaa taatatcgac cacaatagtg
     6841 gtgacgccga gcacgatata ctgcatcagt tgcggctgtt gcggcatgat gaattgcgga
     6901 aatagcgccg ccagaaacac aatacttttg ggattggtga gattcacaaa aactgcgcgc
     6961 tggaacaaat gtcgacgcga ttgagtagag gccagcgatt taaggtcaat tgcaccagcg
     7021 gcgcgccact gctggattcc cagccaaatc aagtaagccg cgcctgccca cttcaacact
     7081 tcaaacgcaa tcactgagcg ggaaaatagc gtccccaacc ccacgccaac cagcacaata
     7141 tgaatcgcca gtccggtctg aagcccagca atagacgcca ccgcgccgcg ataaccgtgg
     7201 ttgagcgagg tggtcatagt gttgattgca ccagagcctg gcgacaggct taaaatgatc
     7261 gatgtcagca ggtaggcaaa ccaccattct aaggtcatga tgaactcccg gtgtgtctat
     7321 ttttgtgcca caatacgcta ctgtcgcagc gttgtgtcag gcacgctaaa aaaaacgatt
     7381 ttacgtggtt taagaggcag attacccgat gtttcagcag caaaaagact gggaaacaag
     7441 agaaaacgcg tttgctgctt ttaccatggg accgctgact gatttctggc gtcagcgtga
     7501 tgaagcagag tttactggtg tggatgacat tccggtgcgc tttgtccgtt ttcgcgcaca
     7561 gcaccatgac cgggtggtag tcatctgccc ggggcgtatt gagagctacg taaaatatgc
     7621 ggaactggcc tatgacctgt tccatttggg gtttgatgtc ttaatcatcg accatcgcgg
     7681 gcagggacgt tccggtcgcc tgttagccga tccgcatctc gggcatgtta atcgctttaa
     7741 tgattatgtt gatgatctgg cggcattctg gcagcaggag gttcagcccg gtccgtggcg
     7801 taaacgctat atactggcac attcgatggg cggtgcgatc tccacattat ttctgcaacg
     7861 ccatccaggt gtatgtgacg ccattgcgct aactgcgcca atgtttggga tcgtgattcg
     7921 tatgccgtca tttatggcac ggcagatcct caactgggcc gaagcgcatc cacgtttccg
     7981 tgatggctat gcaataggca ccgggcgctg gcgcgcgttg ccgtttgcta tcaacgtact
     8041 gacccacagc agacagcgat atcgacgtaa cttacgcttc tatgctgatg acccaacgat
     8101 tcgcgtcggt gggccgacct accattgggt acgcgaaagt attctggctg gcgaacaggt
     8161 gttagccggt gcgggtgatg acgccacgcc aacgcttctc ttgcaggctg aagaggaacg
     8221 cgtggtggat aaccgcatgc atgaccgttt ttgtgaactc cgcaccgccg cgggccatcc
     8281 tgtcgaagga ggacgaccgt tggtaattaa aggtgcttac catgagatcc tttttgaaaa
     8341 ggacgcaatg cgctcagtcg cgctccacgc cattgttgat tttttcaaca ggcataactc
     8401 acccagcgga aaccgctcta cagaggttta aatttcttat gtaccaggtt gttgcgtctg
     8461 atttagatgg cacgttactt tctcccgacc atacgttatc cccttacgcc aaagaaaccc
     8521 tgaagctgct caccgcgcgc ggcatcaact ttgtgtttgc gaccggtcgt caccacgttg
     8581 atgtggggca aattcgcgat aatctggaga tcaagtcgta catgatcacc tccaacggcg
     8641 cgcgcgttca cgatctggat ggcaatctga tttttgctca taacctggat cgcgacattg
     8701 ccagcgatct gtttggcgta gtcaacgaca atctggacat cattactaac gtttatcgcg
     8761 acgacgaatg gtttatgaat cgccatcgcc cggaagagat gcgctttttt aaagaagcgg
     8821 tgttcaaata tgcgctgtat gagcctggat tactggagcc ggaaggcgtc agcaaagtgt
     8881 tcttcacctg cgattcccat gaacaactgc tgccgctgga gcaggcgatt aacgctcgtt
     8941 ggggcgatcg cgtcaacgtt agtttctcta ccttaacctg tctggaagtg atggcgggcg
     9001 gcgtttcaaa aggccatgcg ctggaagcgg tggcgaagaa actgggctac agcctgaagg
     9061 attgtattgc gtttggtgac gggatgaacg atgccgaaat gctgtcgatg gcggggaaag
     9121 gctgcattat gggcagtgcg caccagcgtc tgaaagacct tcatcccgag ctggaagtga
     9181 ttggtactaa tgccgacgac gcggtgccgc attatctgcg taaactctat ttatcgtaat
     9241 cgttctttat ttggtcagtt gtcaacctga tacttcgcta caatggatac tcgttaatca
     9301 aagagttttc cattgtggcg ctacttatca tcaccacgat tctgtgggcc ttctccttta
     9361 gcttttatgg cgagtacctt gcggggcacg tcgatagcta ttttgcggtg ctggtgcgcg
     9421 ttggcctggc ggcactcgtt tttctgccgt ttctgcgtac ccgtggcaat agcctgaaaa
     9481 cggtcggcct gtatatgctg gtgggcgcga tgcagctcgg cgtgatgtat atgctgagtt
     9541 tccgcgctta tctctacctg acggtctccg aattgttgct gttcaccgta ctgacgccgc
     9601 tctacatcac gctgatttat gacatcatga gtaagcgccg tctgcgctgg ggctatgctt
     9661 ttagcgcctt gctggcggtg attggtgccg ggattattcg ctatgatcag gttaccgacc
     9721 atttctggac tggcttgttg ctggtgcaac tctccaatat cacttttgcc atcggcatgg
     9781 tgggttacaa acgcctgatg gaaactcgcc cgatgccgca gcataacgcc tttgcgtggt
     9841 tctatcttgg cgcgttcctg gtggcggtaa ttgcatggtt cttgctggga aatgcgcaga
     9901 aaatgccgca aaccacgttg caatggggca ttctggtgtt tcttggcgtg gtggcttccg
     9961 ggattggcta ctttatgtgg aactacggcg cgacgcaggt ggacgccgga acgctgggca
    10021 ttatgaataa tatgcacgtt ccggcagggc tgctggtaaa cctggctatc tggcaccaac
    10081 agccgcactg gccaacgttc attacaggcg cgctggtgat cctggcctca ctgtgggtgc
    10141 atcgtaagtg ggtcgctccg cgctcttcac aaacggcaga tgatcgcagg cgtgattgcg
    10201 cgctgagcga ataaatgctt ctgtaatcgg ctgacgctgc tcgccatcgc gcacggcggc
    10261 gtacagtcgg ctccacaagc cttcgcccag ggttttggtc accaccagac cttggcgctc
    10321 aaaactctct actacccaat gcggtagcgc ggcaataccc atccgcgcgg caaccatctg
    10381 aatcaacaat aaggtgttat cgacgctttt cagtgacggg ctgacgcctg ccggctgaag
    10441 aaaatgccgc cagacatcca gtcgactacg ctgcaccgga taaatcaaca gcgtctcgct
    10501 ggcgagatct tccggtgtaa ttcgcgtttt cgccgccagt ggatggtcag gtgctaacac
    10561 cagacgcact tcatagtcga acatcggcga ataatgcagg ccactgcgcg gcaggatatc
    10621 ggaggtcatc accagatcca gctcgccctg ttgcaacgca ggctgcgggt caaacgtcac
    10681 gcccgattta aaatccatct ctacctgcgg ccagttctta tggaaatttt ctaacgcggg
    10741 cgtcagccac tgaatacagc tatggcactc aatggcaatg cgcagacgcg tctgctgcgg
    10801 ttcattgcag gcttgcagtg cctggctgat ttgcggcagc acctggtttg ccagttgcaa
    10861 caggatttct ccctgcggtg taaagcgtag cggctggctc ttacgcacaa atagccggaa
    10921 gccaaggcgt tgttccagat cgctaaactg gtgagacagg gcggattgcg tctgatgcaa
    10981 cgtcgccgca gcggctgcga gagagccgca gttccgcaac gcttgtagcg ttttcaggtg
    11041 ttttacttcg atcatgaaag tccttcactt cggcatgaat aatttgcgct tgaggaatat
    11101 acagtaaccg ccaattatgg atgtgtaaac atctggacgg ctaaaatcct tcgtctttta
    11161 aatttatggt gcgttggctg cgtttctcca ccccggtcac ttacttcagt aagctcccgg
    11221 ggatgaataa acttgccgcc ttccctaaat tcaaaatcca taggatttac atataattag
    11281 aggaagaaaa atgacaatat tgaatcacac cctcggtttc cctcgcgttg gcctgcgtcg
    11341 cgagctgaaa aaagcgcaag aaagttattg ggcggggaac tccacgcgtg aagaactgct
    11401 gacagtaggg cgtgaactgc gtgctcgtca ctgggatcaa caaaagcaag cgggtatcga
    11461 cctgctgccg gtgggcgatt ttgcctggta cgatcatgta ctgaccacca gtttgctgct
    11521 gggtaatgtt ccgccacgtc atcagaacaa agacggttcg gtagatatcg acaccctgtt
    11581 ccgtattggt cgtggacgtg cgccgactgg cgaacctgcg gcggcagcgg aaatgaccaa
    11641 atggtttaac accaactatc actacatggt gccggagttc gttaaaggcc aacagttcaa
    11701 actgacctgg acgcagctgc tggaggaagt ggacgaggcg ctggcgctgg gccacaacgt
    11761 taagcctgtg ctgctggggc cggttactta cctgtggctg gggaaagtga aaggtgaaca
    11821 gtttgaccgc cttagcctgc tgaacgacat tctgccggtt tatcagcaag tgctggcaga
    11881 actggcgaaa cgcggcatcg agtgggtaca gattgatgaa cccgcgctgg tactggaact
    11941 accacaggcg tggctggacg catacaaacc cgcttacgac gcgctccagg gacaggtgaa
    12001 actgctgctg accacctatt ttgaaggcgt aacgccaaat ctcgacacga ttactgcgct
    12061 gcctgttcag ggtctgcatg ttgatctcgt acatggtaaa gatgacgttg ctgaactgca
    12121 caagcgcctg ccttctgact ggttgctgtc tgcgggtctg atcaatggtc gtaacgtctg
    12181 gcgcgccgat cttaccgaga aatatgcgca aattaaggac attgtcggca aacgtgattt
    12241 gtgggtggca tcttcctgct cgttgctgca cagccccatc gacctgagcg tggaaacgcg
    12301 tcttgatgca gaagtgaaaa gctggtttgc cttcgcccta caaaaatgcc atgaactagc
    12361 actgctgcgc gatgcgctga acagtggtga cacggcagct ctggcagagt ggagcgcccc
    12421 aattcaggcg cgtcgtcact ctactcgtgt acataatccg gcggtagaaa agcgtctggc
    12481 ggcgatcacc gcccaggaca gccagcgtgc gaatgtctat gaagtgcgtg ctgaagccca
    12541 gcgtgcacgt tttaaactgc cagcgtggcc gaccaccacg attggttcct tcccgcaaac
    12601 cacggaaatt cgtaccctgc gtctggattt caaaaagggc aatctcgacg ccaataacta
    12661 ccgcacgggc attgcggaac atatcaggca ggccattgtt gaacaggaac gtttgggact
    12721 ggatgtgctg gtacatggcg aggccgagcg taatgacatg gtggaatact ttggcgagca
    12781 cctcgacggg tttgtcttta cgcaaaacgg ttgggtacag agctacggtt cccgctgcgt
    12841 gaagccaccg attgttattg gtgacgttag tcgcccggca ccgattaccg tggagtgggc
    12901 gaagtatgcg caatctctga ctgataaacc ggtgaaaggg atgctgactg gcccggtgac
    12961 tattctctgc tggtcgttcc cgcgtgaaga tgtcagccgt gaaaccatcg ccaaacagat
    13021 tgcgctggcg ctgcgtgatg aagtggccga tctggaagcc gctggaattg gcatcatcca
    13081 gattgacgaa ccggctctgc gcgaaggttt accgctgcgt cgtagcgact gggatgcgta
    13141 tctccagtgg ggcgtagagg ccttccgtat caacgccgcc gtggcgaaag atgacacaca
    13201 aatccacact cacatgtgtt attgcgagtt caacgacatc atggattcga ttgcggcact
    13261 ggacgcagac gtcatcacca tcgaaacctc gcgttctgac atggagttgc tggagtcgtt
    13321 cgaagagttt gattatccga atgaaatcgg tccaggcgtc tatgacattc actcgccaaa
    13381 cgtaccgagc gtggaatgga ttgaagcctt gctgaagaaa gcggcaaaac gcattccggc
    13441 agagcgcctg tgggttaacc cggactgtgg cctgaaaacg cgcggctggc cagaaacccg
    13501 cgcggcactg gcgaacatgg tgcaggcggc gcagaatttg cgtcggggat gatgaatatt
    13561 aacgagtcac tgtaaattgc agtacgatga aagaggcaaa actgcctgat actaggccta
    13621 cataatttat gcaatttatt gaatttttgg gttttgttgg ccggacaagg cgttcacgcc
    13681 gcatccggca tgaacaacgc gcactttgcc aataatctga tggcttccat caccggaagc
    13741 cattttgcta tctgcgcccg ttcagataca gaaataaccg aatcattttt acgcaaaata
    13801 taatcctgag cgatttgttc agtaatcaac gctacgttat ttattctcac ttttcacgaa
    13861 attgtatccc ttttcagtaa cgtggtagtg aggcatagac ttatgtccgc tatcctcaat
    13921 aataaccaaa ccctcgttag ctaatttgtt aacaatgtta acaacattac cgaatcctgg
    13981 aatttgcctt atggatagtg tgcgatcgag attcatccag ttccagttta tatctttctc
    14041 agccataatt ttcagtacgt taatttcagt ttcagttatc atagtcaagt gcatcctggt
    14101 ggtataagtc attaagttct cccttaagtt tataatcttc tagagtagca gtatgcgta
//
LOCUS       DAJVQG010000107        14091 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJVQG010000107 DAJVQG010000000
VERSION     DAJVQG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14091
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.107"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            75..2216
                     /gene="ldcC"
                     /locus_tag="OQM83_002852"
     CDS             75..2216
                     /gene="ldcC"
                     /locus_tag="OQM83_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCS1521066.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVEMAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2273..2662
                     /locus_tag="OQM83_002853"
     CDS             2273..2662
                     /locus_tag="OQM83_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1521067.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2727..4025
                     /gene="tilS"
                     /locus_tag="OQM83_002854"
     CDS             2727..4025
                     /gene="tilS"
                     /locus_tag="OQM83_002854"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCS1521068.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4074..4334)
                     /gene="rof"
                     /locus_tag="OQM83_002855"
     CDS             complement(4074..4334)
                     /gene="rof"
                     /locus_tag="OQM83_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCS1521069.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4321..4521)
                     /locus_tag="OQM83_002856"
     CDS             complement(4321..4521)
                     /locus_tag="OQM83_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCS1521070.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4687..5232
                     /locus_tag="OQM83_002857"
     CDS             4687..5232
                     /locus_tag="OQM83_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCS1521071.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5229..5590
                     /gene="arfB"
                     /locus_tag="OQM83_002858"
                     /pseudo
     CDS             5229..5590
                     /gene="arfB"
                     /locus_tag="OQM83_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
     gene            5664..6374
                     /gene="nlpE"
                     /locus_tag="OQM83_002859"
                     /gene_synonym="cutF"
     CDS             5664..6374
                     /gene="nlpE"
                     /locus_tag="OQM83_002859"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCS1521072.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6529..7353)
                     /locus_tag="OQM83_002860"
     CDS             complement(6529..7353)
                     /locus_tag="OQM83_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCS1521073.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7406..9124)
                     /gene="proS"
                     /locus_tag="OQM83_002861"
     CDS             complement(7406..9124)
                     /gene="proS"
                     /locus_tag="OQM83_002861"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCS1521074.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="OQM83_002862"
     CDS             complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="OQM83_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCS1521075.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="OQM83_002863"
     CDS             complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="OQM83_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCS1521076.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="OQM83_002864"
     CDS             complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="OQM83_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCS1521077.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11316..11969)
                     /gene="metI"
                     /locus_tag="OQM83_002865"
     CDS             complement(11316..11969)
                     /gene="metI"
                     /locus_tag="OQM83_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCS1521078.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11962..12993)
                     /gene="metN"
                     /locus_tag="OQM83_002866"
     CDS             complement(11962..12993)
                     /gene="metN"
                     /locus_tag="OQM83_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCS1521079.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13181..13756
                     /gene="gmhB"
                     /locus_tag="OQM83_002867"
     CDS             13181..13756
                     /gene="gmhB"
                     /locus_tag="OQM83_002867"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCS1521080.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3433 a         3562 c         3737 g         3359 t
ORIGIN      
        1 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
       61 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
      121 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
      181 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgtggcgtga
      241 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
      301 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
      361 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
      421 gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct
      481 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
      541 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
      601 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
      661 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
      721 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
      781 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
      841 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
      901 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
      961 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
     1021 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
     1081 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
     1141 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
     1201 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
     1261 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagatggcg gcggcgatgc
     1321 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
     1381 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
     1441 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
     1501 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
     1561 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
     1621 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
     1681 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
     1741 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
     1801 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
     1861 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
     1921 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
     1981 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
     2041 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
     2101 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
     2161 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
     2221 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
     2281 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
     2341 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
     2401 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
     2461 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     2521 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     2581 ccgtgtcgat ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     2641 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
     2701 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
     2761 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
     2821 tggtgcagtg gcggacggaa aatccgggcg tcactctacg cgctatccat gtgcatcatg
     2881 gtttaagtgc taatgccgat gcctgggtta ggcattgcga gaacatctgc caacagtggc
     2941 aggtgccgct ggtggtcgag cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
     3001 aggcgcggca ggcgcgttat caggcatttg ctcgtaccct gttacccggt gaagtgctgg
     3061 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     3121 gcggccctgc cgggctttcg gctatggggg aagtttcgga atttgccgga acacggctta
     3181 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt
     3241 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
     3301 tgcgtgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
     3361 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
     3421 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     3481 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
     3541 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     3601 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaaat
     3661 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
     3721 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
     3781 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
     3841 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
     3901 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
     3961 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
     4021 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat
     4081 tcgcttacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc
     4141 agctcgcggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
     4201 aaatcactgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
     4261 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgca
     4321 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
     4381 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
     4441 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
     4501 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
     4561 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
     4621 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
     4681 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
     4741 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
     4801 cgtatgatgc tgcgcctgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
     4861 cgtggtttgt gtgccgatta tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
     4921 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
     4981 gccgcaaaag tggcgctgtt tacctataat agccgggcgg cgcaaatctg gtggcagcaa
     5041 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
     5101 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
     5161 ggcgtgatct ggttatcgga tgataagaat aatctggaag tgaacttaac cgcctggcaa
     5221 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
     5281 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc
     5341 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg
     5401 ccgccagcca tcatttgatc agcatgatgg cgtaattgtc attaaggcac aggaataccg
     5461 cagtcaggaa ctgaaccgcg aagcggcgct ggcccggctg gtggcagtga ttaaagattt
     5521 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
     5581 gctggcatag aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
     5641 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
     5701 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
     5761 cgcaggctgc cgaactaaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgccg
     5821 attgcgaagg gatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     5881 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     5941 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     6001 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     6061 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     6121 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     6181 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     6241 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     6301 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     6361 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     6421 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     6481 taaggcgttc acgacgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     6541 cccaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa catactgtaa
     6601 ctgcgtttct ggcttaaacg cagagacatc gccagtacgc atatgcatca gatcggcagg
     6661 gctaatccag cctgattgcg ccagcgtaag cggatgcccg gctttggcaa atgcatcagt
     6721 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
     6781 tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     6841 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataactgct
     6901 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     6961 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacgatctg
     7021 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact
     7081 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     7141 cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt gttcggtgaa
     7201 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     7261 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     7321 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     7381 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     7441 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     7501 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     7561 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     7621 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     7681 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     7741 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     7801 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     7861 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     7921 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     7981 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     8041 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     8101 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     8161 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     8221 gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc gctttaacca
     8281 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     8341 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     8401 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     8461 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     8521 tagaaccggt gtcggcttgc acggcgcgga aatccagccc catgcggctg aagattttgc
     8581 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     8641 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     8701 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     8761 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     8821 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     8881 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt
     8941 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     9001 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     9061 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     9121 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     9181 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     9241 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     9301 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     9361 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     9421 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     9481 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     9541 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
     9601 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
     9661 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     9721 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     9781 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
     9841 gccgttggcg ctttttacca gaccaggctg gcgcggaacg gcgaattttt ctttataggg
     9901 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     9961 gcagagccga tacatacagc ctgacgatag cagcctagcg taccgctggt gacttcgcag
    10021 ctatgcagta ataccgcatt ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc
    10081 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
    10141 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    10201 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    10261 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    10321 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    10381 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    10441 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    10501 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    10561 tctttgttat cttcacgcgt tacgatcagg ttcacgtacg gggagtcttt atcttcgaca
    10621 aagataccgt ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact
    10681 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    10741 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    10801 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    10861 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    10921 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    10981 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    11041 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    11101 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    11161 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    11221 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    11281 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    11341 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    11401 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
    11461 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    11521 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt
    11581 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    11641 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
    11701 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
    11761 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    11821 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    11881 atcacaaagc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    11941 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    12001 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    12061 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    12121 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
    12181 cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc
    12241 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
    12301 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    12361 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    12421 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
    12481 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
    12541 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
    12601 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    12661 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    12721 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    12781 cgtggtcaat tcttggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    12841 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    12901 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
    12961 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    13021 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    13081 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    13141 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
    13201 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    13261 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    13321 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    13381 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    13441 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    13501 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    13561 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
    13621 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
    13681 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    13741 aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt
    13801 cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca
    13861 cacaaacagc cggttcggtt gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    13921 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagctgcgtc gcaactgctc
    13981 tttaacaatt tatcagacaa tctgtgtggg cactcgaaga tacggattct taacgtcgca
    14041 agacgaaaaa tgaataccaa gtctcaagag tgaacacgta attcattacg a
//
LOCUS       DAJVQG010000108        13903 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJVQG010000108 DAJVQG010000000
VERSION     DAJVQG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13903
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.108"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            242..1495
                     /gene="kch"
                     /locus_tag="OQM83_002868"
     CDS             242..1495
                     /gene="kch"
                     /locus_tag="OQM83_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCS1521081.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(1550..1723)
                     /locus_tag="OQM83_002869"
     CDS             complement(1550..1723)
                     /locus_tag="OQM83_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCS1521082.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1866..3326
                     /gene="cls"
                     /locus_tag="OQM83_002870"
     CDS             1866..3326
                     /gene="cls"
                     /locus_tag="OQM83_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCS1521083.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3361..3690
                     /locus_tag="OQM83_002871"
     CDS             3361..3690
                     /locus_tag="OQM83_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCS1521084.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDIFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3743..4747)
                     /gene="oppF"
                     /locus_tag="OQM83_002872"
     CDS             complement(3743..4747)
                     /gene="oppF"
                     /locus_tag="OQM83_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCS1521085.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATGGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4744..5757)
                     /gene="oppD"
                     /locus_tag="OQM83_002873"
     CDS             complement(4744..5757)
                     /gene="oppD"
                     /locus_tag="OQM83_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCS1521086.1"
                     /translation="MSVIETATVPLAQQQADVLLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5769..6677)
                     /gene="oppC"
                     /locus_tag="OQM83_002874"
     CDS             complement(5769..6677)
                     /gene="oppC"
                     /locus_tag="OQM83_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCS1521087.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6692..7612)
                     /gene="oppB"
                     /locus_tag="OQM83_002875"
     CDS             complement(6692..7612)
                     /gene="oppB"
                     /locus_tag="OQM83_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCS1521088.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7698..9329)
                     /gene="oppA"
                     /locus_tag="OQM83_002876"
     CDS             complement(7698..9329)
                     /gene="oppA"
                     /locus_tag="OQM83_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCS1521089.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            9613..9909
                     /locus_tag="OQM83_002877"
     CDS             9613..9909
                     /locus_tag="OQM83_002877"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521090.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(10067..10714)
                     /gene="ychE"
                     /locus_tag="OQM83_002878"
     CDS             complement(10067..10714)
                     /gene="ychE"
                     /locus_tag="OQM83_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCS1521091.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11192..13867
                     /gene="adhE"
                     /locus_tag="OQM83_002879"
                     /gene_synonym="adhC"
     CDS             11192..13867
                     /gene="adhE"
                     /locus_tag="OQM83_002879"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCS1521092.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3658 a         3437 c         3230 g         3578 t
ORIGIN      
        1 cttatagccg gtcagtgcat tcactaccac aagcagataa taactttatc tgtatttctt
       61 tatatgatgt gagcatattc aggaaaaatg acctttcctg aggtacagtt gctcgctcaa
      121 atcctttgat tactgcggat aaagtcatct actattgttt aacacattca cgcgctgcat
      181 gtatgttcag aaaatgtgca cgctaagata attaaacaaa attgtggtta cagaggtatc
      241 agtgagtcac tgggctacat tcaaacaaac agcaacaaat ttatgggtga cccttcggca
      301 cgacattctc gcgctggccg tctttttaaa tggattgctt atttttaaaa caatctatgg
      361 tatgtcggtc aatttgcttg atattttcca tatcaaagca ttttcagagt tggatctctc
      421 cttgctggca aacgccccac tatttatgct cggcgtcttt cttgtcctga actccattgg
      481 cttactgttc cgggcaaagc tcgcatgggc aatcagtatc attttgttgt tgatagcgct
      541 aatttacacc ctgcattttt atccctggct gaaatttagt attggatttt gcatttttac
      601 gctggtgttt ttgctgatac tgcgcaaaga cttctcccac agtagcgccg cagccgggac
      661 aatttttgca tttattagtt tcacgacatt actgttttac tcaacctacg gtgcgcttta
      721 tttaagcgaa ggttttaatc cgcgaataga aagtttgatg accgcgttct atttttcgat
      781 agaaaccatg tcaaccgtcg gctatggcga tattgtccct gtttctgaat cagcacgatt
      841 gttcactatt tcggtcatta tttccggcat taccgttttt gccacatcta tgacctcaat
      901 ttttggtccg cttatccgcg ggggattcaa caaacttgta aaaggaaaca atcatacaat
      961 gcatcgtaaa gatcatttta ttgtttgcgg acattcgatt ctcgccatca atacgatcct
     1021 gcaactgaat caacgcggac aaaacgtaac ggttatcagc aacttgcctg aagatgatat
     1081 caagcaactt gagcaacgct taggcgataa cgctgatgtt atccccggtg acagtaatga
     1141 cagttcagta ttaaagaaag cgggaatcga tcgttgccgg gccattctgg cgctgagtga
     1201 taacgatgca gataacgcgt ttgttgtact ctcggcaaaa gatatgagca gtgatgtcaa
     1261 aacagttctc gccgtcagtg atagcaaaaa cctgaataag attaagatgg tacatccgga
     1321 tatcattctc tcaccgcaac tgtttggcag cgaaattctg gcgcgagttt taaatggtga
     1381 agagattaat aatgatatgc tcgtttcaat gttgttgaac tccggtcatg gcattttcag
     1441 cgataacgat gaacaagaaa cgaaagctga cagtaaagaa tcagtgcaaa aatagagttt
     1501 aaagacgaaa gagtaaactt attgggcacc gcaatggtgc ccagtatatt tagatattgt
     1561 agatcgcaaa caataacgag ttacgctgtt tgtttagaat gcacttcctg atgctgtgga
     1621 tacgcatatt tcggcgcgat tgcccctcaa gccaacgcga tttacgtcgg ctagcctgac
     1681 gctgcatgcg ccagcgcccc acttccgttc tactccgctt catgttaact actctatgca
     1741 ataacagaat ggtcattata cccccacccc cgacgctgac cagtgctttt cccgcgtttt
     1801 tatttgccag atgaatccat atgcgtaaac tcataacaat gcgctttcaa aaggatttct
     1861 aatctatgac aaccgtttat acgttggtga gttggttggc cattctggga tactggttgc
     1921 tcattgcagg cgtaacttta cgcattctaa tgaaacgacg cgcagttccc tccgcgatgg
     1981 cctggctgtt gattatttac attctgccgt tagtcggaat tattgcctat cttgccgttg
     2041 gcgagctcca tttaggcaaa cgccgcgctg agcgcgccag agcgatgtgg ccttccaccg
     2101 caaaatggct taacgacctt aaagcctgta agcatatctt cgccgaagaa aatagcagtg
     2161 tcgctgcgcc attattcaag ctttgcgagc gtcgtcaggg gatcgctggg gtcaaaggga
     2221 atcagctaca actgatgacc gagtcagatg atgtgatgca ggcgttaatc cgcgacatcc
     2281 agctcgcgcg ccataatatt gagatggtgt tttatatctg gcagcccggc ggcatggcgg
     2341 accaggtggc tgaatcatta atggcggctg cacggcgtgg tattcattgc cgattgatgc
     2401 tcgactccgc cgggagtgtg gcttttttcc gcagcccgtg gcccgagcta atgcgtaatg
     2461 ccggtattga agtggtcgaa gccttaaagg tcaatctgat gcgtgtgttt ttacgccgta
     2521 tggacctgcg ccaacatcgc aagatgatca tgatcgataa ttacatcgcg tacaccggca
     2581 gcatgaatat ggtcgatcct cgctacttca aacaagatgc gggcgtaggg caatggattg
     2641 atctgatggc gcgtatggaa ggccccatcg ccaccgcgat ggggattatt tattcctgcg
     2701 actgggagat tgaaaccgga aaacgtattc tgccgccacc accagatgtc aatattatgc
     2761 cgtttgaaca ggccagcggt cacaccattc acacaattgc ttctggcccc ggctttccgg
     2821 aagatctcat tcaccaggca ttattgactg cggcttattc ggcgcgtgaa tatttgatca
     2881 tgacaacgcc ctactttgtg ccaagcgatg atttacttca tgcgatttgc acggcggcgc
     2941 agcgcggggt ggatgtcagt attatccttc cgcgaaaaaa tgactcgatg ctggtcggct
     3001 gggccagtcg cgcattcttt acggaactgc ttgctgctgg ggttaaaatt tatcagtttg
     3061 aaggcgggtt actgcatacc aagagcgtgc tggtcgatgg cgaactaagt ctggttggca
     3121 ccgttaacct tgatatgcgt agtctgtggc tgaatttcga gattaccctg gcaatcgacg
     3181 ataaaggttt tggtgcagac cttgccgccg ttcaggacga ttatatttcg cgttcacgtc
     3241 tgctcgatgc ccgtttatgg ctaaaacgtc cattatggca acgtgtcgcc gagcgactgt
     3301 tttacttctt cagcccgttg ctgtaaaacg tcgccaacag aggttaaaca ggtagtgact
     3361 atggatatgg atctaaacaa tcgcctgact gaagatgaaa cgcttgagca ggcctacgat
     3421 atttttctcg aactggctgc cgacaatctc gacccagctg atgttctgct gttcaatctt
     3481 cagtttgaag agcgcggcgg cgcggagtta ttcgatccgg cggaagactg gcaggaacat
     3541 gttgatttcg atttgaaccc ggactttttt gccgaagtgg tgattggtct ggcggacagt
     3601 gaggatggtg aaatcaacga tattttcgcc cgcattctgt tatgccgcga aaaagatcac
     3661 aaactttgcc atatcatctg gcgggaataa ataatcgctt tcggcgtgaa aaagggctga
     3721 caactgtcag cccttattgt gtttaaagcg gatcgacttt caggcaagaa acggcgtgtc
     3781 tgaaactccc ttccagcaca ggacgtgttt tggcgcactc cggaccggca atcgggcaac
     3841 aggtacggaa aacacaaccg gaaggcgggt tgatcggcga cggtaattcc ccttccagta
     3901 actggatggt tttgttcttc tccagatccg gatcaggtat ggggactgcc gacatcaatg
     3961 ccctggtgta aggatgtagt ggattgtggt agacctcatc ataggtcccc agttctaccg
     4021 catggccgag atacatcacc aacacacgat cggaaatgtg ttttaccacg gccaggtcat
     4081 gagcgataaa aattaatgac aatcccatct cacgttgcag ctgctggagc aggttgacca
     4141 cctgcgcctg aattgacacg tccagcgccg aaatcggctc atcgcagata atcagcttcg
     4201 gttcaagaat aagagcacga gcaatcccga tacgctggca ctgcccaccg gagaactcat
     4261 gcggatagcg gttaatcagg ttaggcaata acccgacttt cagcatcatc gccttcacgc
     4321 gctcgcgaac ttcctggcgt gacattttcg gatgataagt acgcagtggt tcagcgatga
     4381 tctcgccgat ggtcatacgc gggtttagcg atgccaacgg atcctggaaa atcatctgaa
     4441 tatcactgcg aacggcacgc cattcatcgg gcttcatgcc cagcaactct ttacctaacc
     4501 aggcaacatg accgccggtc gctttaacca ggccaatgat ggcgcgagca aaggtggact
     4561 taccgcatcc cgattcccct accacaccta atgtttcccc ttcatacagg cgaagcgtga
     4621 caccatcgac ggctttgaga gttttcggcg gttgccagaa ccactgtttg ccatctttga
     4681 tttcaaagtg cactttcaga tcggcgattt cgaggaggac ttttcttcct tcggttacag
     4741 cattcataac agttcctcca ccggtttaaa gcaagcacgc agacggccag gcgtaaactc
     4801 ttccagcggc ggtgcgctac tacaaatttc catcgcatgc ggacaacgtg gctggaacgg
     4861 gcaacctttc ggtaatcgca gcaggtttgg cggattacca gggatggtca acattgtttc
     4921 accttccgca tcgagacgcg gcaccgcgtt gagcaaaccg atagaataag gatgaacggg
     4981 ttgataaaag acatcgcgcg cgttgccata ttccatcgtg cgcccggcgt acattaccag
     5041 cactttgtcg cagatccccg ccaccacgcc aagatcgtgg gtaatcataa tgatggcggt
     5101 attaaattcc cgcttcagtt catttaatag cgtcatgatc tgcgcctgta cggtgacgtc
     5161 cagcgcagta gtgggttcat ccgcaatcag cagcttaggt cgacatagca atgccatcgc
     5221 aatcataact cgctgacgca tgccaccaga aaattcgtgc gggtacattt tcatgcgttt
     5281 acgtgcttcc ggcattttta ccgcatcgag catccgcacc gactcttcaa acgcctcagc
     5341 tttgctcatg ttcttatgca gcatcagcac ttccatcaac tgctcaccga cgcgcatata
     5401 gggattcaac gaagtcattg ggtcctgaaa aatcattgag atttgttcag cgcgcagttt
     5461 attgagttca cgctctggca aattgaggat ttcacgccca ttgaaggttg ccgatccgcc
     5521 aatacgtccg ttggcagcca acaggcccat caacgcaaat gcagtttgcg atttacccga
     5581 accagactca cctacaatgc ccagcgtttc tccggcacgt agagaaaaat tcaaatcatt
     5641 gaccgccgtg acatcgccgt ccggggtact aaaggtgaca cgcaaatctt tcacgttcag
     5701 cagtacgtca gcctgttgtt gtgcgagcgg cacagttgca gtttcaatta cgctcatggc
     5761 tgcactcctt aacgatcttt cgggtcgaga gcatcacgca agccatcgcc gataaagttg
     5821 aaacaaaaca gcgtcaccac gaggaatccc gctgggaaca acaataacca tggagagact
     5881 tccatcgagt tcgcgccatc actcagcaat gcaccccagc tgcttaacgg ctcttgcgta
     5941 cccaacccca ggaagctaag gaaagattca aagaggatca tgctgggcac cagaagtgat
     6001 gcgtagacca ccaccacacc gagtacattc ggcacaatgt ggcgaataac aatgcccgat
     6061 gtcgatacac cgccaacttg tgccgcctca ataaactctt tgcgcttcag actcagggtt
     6121 tgtccacgca caatacgagc catatccagc caggaaacca tgccaatcgc cacgaaaatc
     6181 agcaggatgt tttgaccgaa aaaggtcaca agcaaaatga cgaagaacat gaatgggaag
     6241 gagttgagga tttccagcag acgcatcatt accgaatcca ctttaccgcc cagataaccg
     6301 gaaagcgaac cgtaaagtgt ccccacgacc actgccacca gtgccgcagc aacaccgacc
     6361 atgagtgaga tacgcccgcc aatcgcaacg cgcacaagca ggtcgcgacc ggatgagtca
     6421 gtaccaaagt agtgaccgga ctccatatcc ggggcgctgg acatcatcgc ccagtcagta
     6481 tcgtcatagg caaactgcga aagcatcggt gccaggatta caaataacgc gatcagcacc
     6541 agcactatca gactggcaac cgccgcacgg ttatgcataa aacgtcgacg tgcgtcctgc
     6601 cacaagctgc gcccttcgac ctccagcttt tcactgaaat tttccagcgt ctcgctgttt
     6661 ttcttactta acatcatcgc gagctccagt atcagtaacg gattttcggg tcgataaccg
     6721 catatagcac atcgacaatg gcattaaaca aaatggttaa agcaccaacc aggatggtca
     6781 ggcttaacac taaggaatag tcacggttca atgcgccatt aacgaacagt tgtccaatcc
     6841 ccggcaaacc ataaatggtt tcgataacca tagaaccggt aataatgccg acaaatgcgg
     6901 ggcccatata agagagcacg ggtaacagag caggttttaa tgcgtggcgt aaaatgatcc
     6961 gccgcatagg taaccctttc gcccgggcag tacgaataaa gttggagtgt aatacttcaa
     7021 tcatagagcc tcgggtaata cgcgcaatac tggcgatata agcgagtgac aacgccacca
     7081 tcggcaatat catgaattta agcgccccac cattccagcc accgcccggc agccaatgca
     7141 aaatgatcgc aaatatcatg actaataatg gcgcaaccac aaaactgggg ataacaaccc
     7201 cggtcattgc cagccccatc acggtatagt cccatttggt gttttgtttt aatgcgacaa
     7261 taacgccagc actaacaccc agtattaccg caaggaaaaa tgcggcggct cccagtttgg
     7321 cagaaacggg aaaactggat gcaaccaggt cattgaccga ataatcttta tatttaaacg
     7381 atggaccgaa atcaccgtgc gccagttgtt tcaggtagct gaaatactgt gtcatgatcg
     7441 gatcattaag atgatatttc gcttcgatat tggccatcac ttccggcggt aaagtacgtt
     7501 cgccggtaaa agggcttccc ggcgcgaggc gcatcataaa gaacgaaata gtaataagaa
     7561 taaatagcgt cggaatcgct tccagacagc gacgtagaat aaattttaac attgccctac
     7621 cttctggcct gtgccttctg taatgcgata aaaatcagac accgtggagc aggacactcc
     7681 tgccccacgt attgccatta gtgcttcaca atgtacatat tccgggtgta ggtattatcc
     7741 agcggatctt tgccggtata gccaccaacc cacggtttca ccagacgcgc attcacgtag
     7801 taataaacag gaacaatggc cgaatcctta tccagctgtt gttctgcttt agtgtacaga
     7861 gctgtgcgct gcgcctcgtc agtcactttc agcgtttccg ccataatgct gtcaaaggcc
     7921 gggctcttat aatgcgcggt attcatcgag ctgttcgaca gcatggtgtt caggaaggaa
     7981 gttggttcgt tgtagtctgc acaccagcct gcacgggcca catcaaaagt gccctggtga
     8041 cgggtgtcga ggaacgtttt ccactcctgg ttaaccagtt tgacgtttac accaatgttt
     8101 ttcttccaca atgaagaggc agcaatcgcc agctttttat gcagatcaga ggtgttatac
     8161 agcaggttga tggtcaacgg tttgtctgcg gtataacccg cttcagccag cagttttttc
     8221 gcttcttcgt tacgtttttc ctggctccag ccaaaccatt ctggctgagt caattttgcg
     8281 ccatcagtat acggtggagt gtaaccatag gcgggcatgt cgccctgcgc tttcacttta
     8341 ttaacaatga tatcgcggtc catacctagt ttcagtgcgg tacgcacacg cacatcgttg
     8401 aatggcggtt tctggttgtt aatttcgtaa tagtaagtgc acaggtatgg atcaacgtga
     8461 acttcgtccg ggatctcttt tttcagcttc tggaacaatt cgatcggcat gttgttatac
     8521 gtcatgtcga tttcaccact acggtagcgg ttgacatcgg taacttcaga agcaataggc
     8581 aaataggtta cctggttaat aacggttttc gcgttgttcc agtaggtcgg gctgcgttca
     8641 agaacgattc gttcgtttac gacccaatct tttaaggtat aggcaccgtt ggtgacgata
     8701 ttaccaggct gggtccattt ttcgccgaat ttctcgatag cggcttttgg caccggtgaa
     8761 gttgatgggt gaacaagtaa tttatagaag tacggaacgg gttcacttaa ggtgacttct
     8821 aatgtgtgat catcaatagc tttcacgccg agatcggtaa tcggtttttt cccttcaaga
     8881 atttcatcaa taccggcgat atgcccatat tgcagataac tggcatacgg agaagcagtg
     8941 ttcggatcaa cagaacgttg ccagctatac acaaagtctt gtgctgtgac tggcgtgcta
     9001 tcagaccatt tcgcatcttt acgcaaatgg aaggtccaga ctttcgcgtc tttattatcc
     9061 caggattcag cgacgccagg tgctggatga ccgtcaagat cgctgaccag taagccttca
     9121 aacaggtctc ggctgatatt agactccgga acaccttcaa ttttgtgcgg atctaatgac
     9181 tgaacttctg aaccattgtt acgtaccagt gtttgttttt ccgccagtgt gacgcctgcg
     9241 ggtacatcag ctgccagcgc gacattccct gccattagcg cagccagaac gccagctgct
     9301 actaaacttc tcttggtgat gttggtcatt gttttttgga ctccctcatt ataattactg
     9361 gtattaacca gcatgtgtaa tcccggattg ggaccctgca caggctcgga gactttctgc
     9421 tgtcaggtta ctttatcgtt acgtattact atcaccgact ttatttattc tggtgattca
     9481 gacgatcacc ttatgtcgat tcgtactgtg tctcccctgt cgagacacat tattctttcg
     9541 cgacgctctt taattgttaa ttattctcat taccaataaa gaatcgtctg gcggttgccc
     9601 ggaaagtacc aaatgcggtt cttactcgcc aatacatttt gcaaaattgt taagcaattc
     9661 tcttttattg accgtatcag aatctatagc ttgcatccat gccttaacaa acatctttca
     9721 attcaatcaa atagagattg gctaagcatg tctatctttt ctgattacag ttccagtagt
     9781 gaaatgcaca ataatttaac aattgattat tatttagcac tttcctctac caaaggcagt
     9841 ggaattacta atatcatttc aattattctt cagcaagccc aggattatga tgtggcgaaa
     9901 ataacatgat ggtgaggaga agcagttaag gaaatcctta taactaactg taatttgaga
     9961 agaatatttt tacctattcc ttttgctttt gataactgcg cgggaacaaa gtaaaaataa
    10021 tgacgaagta aacggagcat ggcagctccg tttcatttga aaggaattaa ttaagcaggc
    10081 cggggaaaat ccccttaata ccagtaacga taaattcaat ccccaatgcc atcagcaata
    10141 gccccataat acgcgtaatc acgttgatgc cggtctggcg taaaacccgt accagccacg
    10201 gtgccatgcg gaacaatccc cagcaacata aagcgaacaa tgcaatagcc acaaagaaac
    10261 caaacagata gctaatgctg tgataacgcg taccccagac gatggtagaa ctgatcgccc
    10321 ctggccccgc catcaacggc aacgccaatg gcaccacacc aatgctttca cgtaccgcgg
    10381 tttctgattt ttcttgcttg ttctgtttat cctcgccaag cttgccgctg atcatcgaca
    10441 tcgctatcgt caccaccagg atgccccctg cgatacggaa cgaatcaatt gatataccaa
    10501 aaagttgcag aatcgtgtcg ccgagaaaaa gcgagatcca caagataatg gccacagaca
    10561 ggttggctgt aaggttagtt ttgtttcgcg ctgccgctgt ctgataactg gtcatgctga
    10621 taaagacggg aataatccct accgggttga ccagcgcaaa taacccgatg aaaaatttga
    10681 agtaaacggg aaaatcaaaa aaggtctgaa tcacggttag ctccgaagca aaagccggat
    10741 aatgttagcc ataaattaag gttgaaaaga cgcgctgaca atacgccttt tgacagcatt
    10801 tttcacctcc taactactta aaattgctat cattcgttat tgttatctag ttgtgcaaaa
    10861 catgctaatg tagccaccaa atcatactac aatttattaa ctgttagcta taatggcgaa
    10921 aagcgatgct gaaaggtgtc agcttagcaa aaatttgatt tggatcacgt aatcagtacc
    10981 cagaagtgag taatcttgct tacgccacct agaagtgacg cattagagat aataactcta
    11041 atgtttaaac tcttttagta aatcacagtg agtgtgagcg cgagtaagct tttgattttc
    11101 ataggttaag caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac
    11161 taaaaaagtt taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa
    11221 cgcactcgta gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca
    11281 agtagacaaa atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc
    11341 gaaaatggcc gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca
    11401 ctttgcttct gaatatatct acaacgccta taaagatgaa aaaacctgtg gtgttctgtc
    11461 tgaagacgac acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat
    11521 cgttccgacc actaacccga cttcaactgc tatcttcaaa tcgctgatca gtctgaagac
    11581 ccgtaacgcc attatcttct ccccgcaccc gcgtgcaaaa gatgccacca acaaagcggc
    11641 tgatatcgtt ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat
    11701 cgatcaacct tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat
    11761 cctcgcgact ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat
    11821 cggtgtaggc gcgggcaaca ctccagttgt tatcgatgaa actgctgata tcaaacgtgc
    11881 agttgcatct gtactgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca
    11941 gtctgttgtt gttgttgact ctgtttatga cgctgtacgt gaacgttttg caacccacgg
    12001 cggctatctg ttgcagggta aagagctgaa agctgttcag gatgttatcc tgaaaaacgg
    12061 tgcgctgaac gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt
    12121 ctctgtacca gaaaacacca agattctgat cggtgaagtg accgttgttg atgaaagcga
    12181 accgttcgca catgaaaaac tgtccccgac tctggcaatg taccgcgcta aagatttcga
    12241 agacgcggta gaaaaagcag agaaactggt tgctatgggc ggtatcggtc atacctcttg
    12301 cctgtacact gaccaggata accaaccggc tcgcgtttct tacttcggtc agaaaatgaa
    12361 aacggctcgt atcctgatta acaccccagc gtctcagggt ggtatcggtg acctgtataa
    12421 cttcaaactc gcaccttccc tgactctggg ttgtggttcc tgggggggta actccatctc
    12481 tgaaaacgtt ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa
    12541 catgttgtgg cacaaacttc cgaaatctat ctacttccgc cgtggctccc tgccaatcgc
    12601 gctggatgaa gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct
    12661 gttcaacaat ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac
    12721 tgaagtcttc ttcgaagtag aagcggaccc gaccctgagc atcgttcgta aaggtgcaga
    12781 actggcaaac tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga
    12841 cgccgcgaag attatgtggg ttatgtacga acatccggaa actcacttcg aagagctggc
    12901 gctgcgcttt atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc
    12961 gaaaatgatc gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt
    13021 tgtaactgac gacgctactg gtcagaaata tccgctggca gactatgcgc tgactccgga
    13081 tatggcgatt gtcgacgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg
    13141 tggtctggac gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt
    13201 ctctgatggt caggctctgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta
    13261 ccacgaaggg tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag caactatcgc
    13321 gggtatcgca tttgcgaacg ccttcctggg tgtatgtcac tcaatggcgc acaaactggg
    13381 ttcccagttc catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg
    13441 ctacaatgcg aacgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca
    13501 ggctcgccgt cgttatgctg aaattgccga ccacttgggt ctgagcgcac cgggcgaccg
    13561 tactgctgct aagatcgaga aactgctggc atggctggaa acgctgaaag ctgaactggg
    13621 tattccgaaa tctatccgtg aagctggcgt tcaggaagca gacttcctgg cgaacgtgga
    13681 taaactgtct gaagatgcat tcgatgacca gtgcaccggc gctaacccgc gttacccgct
    13741 gatctccgag ctgaaacaga tcctgctgga tacctactac ggtcgtgatt atgtagaagg
    13801 tgaaactgca gcgaaaaaag aagccgctcc ggctaaagct gagaaaaaag cgaaaaaatc
    13861 cgcttaatca gtagcgctgt ctggcaatat aaacggcccc ttc
//
LOCUS       DAJVQG010000109        13892 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJVQG010000109 DAJVQG010000000
VERSION     DAJVQG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13892
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.109"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..1156)
                     /gene="hprS"
                     /locus_tag="OQM83_002880"
     CDS             complement(<1..1156)
                     /gene="hprS"
                     /locus_tag="OQM83_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCS1521093.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNID"
     gene            complement(1156..1827)
                     /gene="hprR"
                     /locus_tag="OQM83_002881"
     CDS             complement(1156..1827)
                     /gene="hprR"
                     /locus_tag="OQM83_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCS1521094.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1960..2373
                     /gene="hiuH"
                     /locus_tag="OQM83_002882"
     CDS             1960..2373
                     /gene="hiuH"
                     /locus_tag="OQM83_002882"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCS1521095.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2482..3486
                     /gene="msrP"
                     /locus_tag="OQM83_002883"
     CDS             2482..3486
                     /gene="msrP"
                     /locus_tag="OQM83_002883"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HCS1521096.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            3487..4122
                     /gene="msrQ"
                     /locus_tag="OQM83_002884"
     CDS             3487..4122
                     /gene="msrQ"
                     /locus_tag="OQM83_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCS1521097.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            4379..5029
                     /gene="zinT"
                     /locus_tag="OQM83_002885"
     CDS             4379..5029
                     /gene="zinT"
                     /locus_tag="OQM83_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCS1521098.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            5372..5902
                     /locus_tag="OQM83_002886"
                     /pseudo
     CDS             5372..5902
                     /locus_tag="OQM83_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(6472..6561)
                     /locus_tag="OQM83_002887"
     tRNA            complement(6472..6561)
                     /locus_tag="OQM83_002887"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6525..6527),aa:Ser,seq:cga)
     gene            6655..7452
                     /gene="mtfA"
                     /locus_tag="OQM83_002888"
     CDS             6655..7452
                     /gene="mtfA"
                     /locus_tag="OQM83_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCS1521099.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            7553..7628
                     /locus_tag="OQM83_002889"
     tRNA            7553..7628
                     /locus_tag="OQM83_002889"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7586..7588,aa:Asn,seq:gtt)
     gene            7942..13892
                     /gene="yeeJ"
                     /locus_tag="OQM83_002890"
                     /pseudo
     CDS             7942..13892
                     /gene="yeeJ"
                     /locus_tag="OQM83_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
BASE COUNT         3929 a         3302 c         3128 g         3533 t
ORIGIN      
        1 catcaatatt aagatagccg ttggtatcaa gaaaactggt tatatgaata cgcgattttt
       61 ctggtgaata tctaatggca ttaacaataa gattcgataa cattcgttgc agtaaaattt
      121 tatccgcaaa gatttgctga ttgcactcga ccttaaagca aatctctttc tcgtctgaaa
      181 ggtattcaag ataatccaac aaattttcga cttccttatt gagagaaagc gagtccagtt
      241 tcaccaaaac attgttttta tctgcccggg caagaaacag tatgttctct gttaaccgcg
      301 aaatattttc cagctcttca atgttaccgg caattgtttt ttgatattca gcgatacttc
      361 tggtttgact gagcgtaacc tgattctgac ccagtaatgc attaatgggc gttctaagtt
      421 catgagcgag atcgtcagca aattgactta gacgctcaaa atctttgact aaagcatgat
      481 gcattttatt caacgcctgc ccaagaggtt ttagttctct cggtaatgcg ctaacctcaa
      541 caggctcccg gctatcgtta taattcagcg cttccgttac accactcaac tttttgatct
      601 ctcgtaatcc cgttctgatt aacagcggac ttaatactga gcaaagtaca atggcgacaa
      661 tgcaaattat aatgctatta attttatact gttcaagcat gttatgtctg gctgaagcca
      721 atttagccac agtaaccgtt aatgatggcg aaacttcatc aatattaatt cgtaaagcat
      781 caatctcagt atcattaata atgcttctgt aaatgccagc tgcgctgatt gtctcactag
      841 caggtatgtt atttaacatg tcatcactga catttgtccg gttaacaatt ttattgatgc
      901 catcaccatg aatgatcaag atatcctgac taacatccat catccggtta aagtatacag
      961 gtaacgtatc tggatttacc ccatcaatta acaactgctt gatctgtgct gtccggttaa
     1021 tgagtgttgt atcatcgcgc catttcaact cacttgccag gccattatag agagtccaga
     1081 caattccagc gccagcaaca gacagcagca atataaaaag caaggttaaa cggacggtta
     1141 tagataatct tttcattatt tttttaccgc tacgaatgaa tagcccatcc cccggattgt
     1201 ggcgattagc ttttcaggaa aaggatcatc aacttttgcg cggagcctgc gaatggcgac
     1261 atccaccgta ttggtatcac tatcaaagtt gattccccaa atttcactcg caataaccgt
     1321 tctgggtata atttcgccag ctctggaggc cagtagccaa agtaactgaa actccttgcg
     1381 cgtcagtgta atactgatat tgttcctgct cacactttga cttacagagt ccattcttaa
     1441 gccactgatt tctaatgttg aattcaaagc gtgatgttgc cttaattgtg cccgaaccct
     1501 tgccagcaac tcagaaaatg aaaaaggttt taccagataa tcatttgctc cactgtccag
     1561 ccctctgact ctgtcatcga cagaatccct tgcagtaagg caaataacag gggtttgctt
     1621 tgctgttctt aacgtttgta agatctgcca gccatccata cccggaagca taatatccag
     1681 aatgatcaat gcataatcat ccttcagcgc aagataaagc ccatctctgc catcagaaac
     1741 ggcatcaatg acataacccg cttcggaaag cccctgcgtt acccactcct gggtcctttg
     1801 attatcttca ataagtaaaa tcttcatgaa aataatatgc cataaatcat atgttataga
     1861 aacagcctag ttcattacaa aattgtaatg ctgctgtaag gttaccctgg ccgctttttc
     1921 gctatcctca aaactcattc acatgacaag gatataaaca tgttaaagcg ttatttagta
     1981 ctctccgtag taacggcagc attttcatta ccttctttgg tttatgccgc acaacaaaac
     2041 attcttagcg tgcacatttt gaaccagcaa accggaaaac ccgctgccga cgtgacagtc
     2101 actcttgaaa agaaagcgga caacggctgg ttacaactta ataccgccaa aacagataag
     2161 gatggacgaa ttaaggcact gtggcccgag caaactgcaa ctacgggcga ttaccgtgtc
     2221 gtatttaaaa ccggggacta tttcaagaaa caaaatcttg aaagtttctt ccctgagatc
     2281 cccgttgaat ttcatattaa taaagtgaac gagcattatc atgtgccttt attacttagc
     2341 caatatgggt attcaaccta tcgtggcagt taatgcataa atatgtaaaa gcagatctct
     2401 gctactgtta gggaggacac tccctgacag attaacagta aacggctctt gctggctaac
     2461 gacaaaaaag tgtgatggct tatgaaaaag aatcaatttt taaaagaatc agatgttacg
     2521 gccgagtcgg tattctttat gaagcgtcga caggtgttaa aagcactggg catcagcgca
     2581 gctgcacttt ctttgcctca cgctgcgcat gccgatctgc ttagctggtt taaagggaac
     2641 gatcgcccgc ccgcccccgc cggaaaaccg ctggagttca gcaagcctac cgcctggcaa
     2701 aataatctgc cactgacgcc agtagataaa gtctccggtt ataacaactt ctatgaattc
     2761 gggctggata aagccgatcc cgccgctaat gctggtagcc tgaaaaccga tccatggaca
     2821 ctgaaaatca gcggcgaagt ggcaaaacca ttgaccctcg atcacgatga tttaacccgt
     2881 cgcttcccgc tggaagagcg tatttatcgt atgcgctgcg tggaggcatg gtcgatggtg
     2941 gtgccgtgga ttggttttcc gctgcacaaa ttgctggcgc ttgccgaacc caccagcaat
     3001 gcgaagtatg tcgctttcga aacaatttat gcaccggaac aaatgccagg ccagcaggac
     3061 cgctttatcg gcggcgggct gaaatatcct tatgtcgaag gattgcgtct cgacgaagca
     3121 atgcatccgc tcacactgat gaccgtgggt gtttatggca aggcgttacc gccacaaaat
     3181 ggcgcgccgg tacgactgat tgtgccgtgg aaatatggct ttaaagggat taaatcgata
     3241 gtcagtatta agctgacccg cgagcgtccg ccaaccacct ggaatctggc agcgcctgac
     3301 gaatacggtt tttacgccaa cgttaatccg catgttgatc acccgcgctg gtcacaggct
     3361 accgaacgat ttattggttc aggcggcatc ctcgatgttc agcgccagcc aacgctactg
     3421 tttaatggtt acgccgacca ggtggcatcg ctgtatcgtg gcctggattt gcgggagaat
     3481 ttctgaatgc gtctgacagc aaaacaggtg acatggctga aagttagcct gcatcttgcc
     3541 ggattgttgc cgtttctctg gctggtctgg gcgatcaatc acggtggact gggtgccgat
     3601 ccggtgaaag atattcagca ttttaccggt cgcactgcac tgaaattttt gctggcgacc
     3661 ttgttaatca cacctctggc acgctacgca aaacagccgt tattgatacg cactcgccgc
     3721 ctgttaggct tatggtgctt tgcctgggcg acactgcatt taaccagtta cgcattgctg
     3781 gagctgggcg tgaataatct ggcgttatta ggcaaggagt taattacccg gccttattta
     3841 acgttaggaa ttatcagctg ggttattttg cttgctttag cgtttacttc aacccaggcg
     3901 atgcagcgaa aactcggcaa gcactggcaa cagttgcata atttcgtcta tcttgtcgcg
     3961 atcctggccc caatacatta tctgtggtca gtgaagatta tttcaccgca gccgctcatc
     4021 tacgctgggc tggctgtact gcttttagcc ttacggtata agaagttgcg ttcccttttt
     4081 aagcggttac gcaagcaggt tcacaacaaa ttatctgtat aacttcatat tgccgacaaa
     4141 gtacgcgtta tccctgccgg atgtggcgcg agcgccctgt ccggcctgcg cagatcacat
     4201 caggttatgt ttcgtttgtc atcaacctca aattatcaac ataattcatt ttatttcaat
     4261 aagatagata agtacacctg agaaaactat gctctcatct cctaagagtt gttgcatttt
     4321 gccatatgtt acaatataac attacacatc atatacaatg actctggagg aaactgtttt
     4381 ggcaattcgt cttcacaaac tggctgttgc tttaggtgtc tttattgtta gcgctcctgc
     4441 cttttcgcat ggtcatcact cacacggcaa acccttaaca gaggtcgaac aaaaagctgc
     4501 taatggtgtt tttgatgatg ccaatgtaca aaaccgaacg ctcagtgact gggatggagt
     4561 ctggcaatcc gtttatcctt tactgcaaag tggcaaactt gaccccgtct ttcagaagaa
     4621 agcggatgca gataaaatta aaacatttgc tgaaattaaa gattattatc acaaaggtta
     4681 tgcaacagat atcgagatga ttggcattga ggacggcatt gttgaattcc atagaaataa
     4741 tgaaacaaca tcctgtaaat atgattacga tggatacaaa atactcacct ataaatcagg
     4801 caagaaaggc gttcgttact tattcgaatg taaagatcct gaaagcaaag cccctaaata
     4861 tatacaattt agcgatcata taattgcacc acgaaaatca tctcattttc acatttttat
     4921 gggtaatgat tcacagcaat ttttattaaa tgaaatggaa aactggccaa cgtattatcc
     4981 atatcagttg agtagcgaag aagtggtcga ggaaatgatg tctcattgag tattctcatg
     5041 ataacgcctc gatgccgctt tagtaagtca tcataactgc cactggtcca tccacaaacg
     5101 ccactgaacg caagctagct acagacacgc tcatcactat gacgtgtctg tatattaata
     5161 agctaacccg cattgagtta accaataacg gattccatac acaatacggc caacagagaa
     5221 agatacctgt gctcacgcca ttgcttatat tggctggtta caatgtgcac tatcaatttt
     5281 ttaaataaca aattcttaaa accacatctg tataaggaat ttttaaggtt cgtgggatag
     5341 cttgactgta aaaattacag gagctacaaa aatgaaccga ttctcaaaaa ctcaaattta
     5401 tttacattgg ataacgctgc ttttcgttgc aataacctat gccgcgatgg aactccgtgg
     5461 ctggtttcct aaaggtagta gtacttatct gctgatgcga gaaacacatt acaatgcggg
     5521 tatattcgtt tgggtgttaa tgttttcacg cctgattata aaacaccgtt atagtgatcc
     5581 ttctattgta ccaccgccac ctgcctggca aatgaaagcg gcttcgctaa tgcacatcat
     5641 gctttatata accttccttg cacttcctct gctggggatt gctttgatgg cttacagtgg
     5701 aaaatcgtgg agtttccttg gtttcaatgt gtctcccttt gttaccccaa acagcgaaat
     5761 taaagcactg ataaaaaata ttcacgaaac ctaggcaaat ataggttact ttttaatcgc
     5821 agctcacgct ggcgcagcac tctttcatca ctacattcag aaagataata ctctgttacg
     5881 aatgatgcat cgccgcaaat aagtcgatcc cctgacaagg aaatgtgaac catgtacatc
     5941 aacgcgtcat aaatgtttac gcaagtgcac aatgcatcct ttctactatc aacaaggagg
     6001 agcagaaact gtgtaaatcg tacaatggta agacttatca aaaatacagg ttaattttgg
     6061 tatttaaacc ttttttactt gttagtgtga tacaaaggct ttcaaaaaag ctgggaaatt
     6121 caacaactct tggaaattat ggactttgtc ccgcggcttt aatctgacgg ccgcgttcct
     6181 tttttgactc aattattgac cccttctcta cgcaacttca gttcccacca ccaactttgc
     6241 ggcagtttca taggatgaat gtctaaaaga ataatggtga gcgataagaa aacgactgaa
     6301 taactgcaca ttttcgctcg aaagcttcct gtcagatcca tagcgaatca agtgctgaat
     6361 gtcacagtat cgaacagaaa acagtgacga tctaaccctt caagaatatt ctacaattgt
     6421 tctgtttagg aaaagcaagg cgggaagtca ggagataagt cattgataaa gtggcggaga
     6481 gagggggatt tgaacccccg gtagagttgc ccctactccg gttttcgaga ccggtccgtt
     6541 cagccgctcc ggcatctctc cgttcagatg gttgccatga tgccaggaaa tttggcattt
     6601 taacagtccc tgttcgtgca attttgttca agtgacgagt ttgcgagcaa aacgatgatt
     6661 aagtggccct ggaaagtaca agaatcagca catcaaactg cccttccctg gcaggaagca
     6721 ctatcgatcc cccttttaac gggtctgacg gaacaggaac aaagcaaatt agtcactctt
     6781 gccgaacgtt ttttacagca aaagcggctt gttcctttac agggctttga actggattca
     6841 ttaagaagct gccggatagc acttctattt tgcctacccg ttctggagtt aggactggaa
     6901 tggctggatg gttttcatga agtcttaatt tatcctgcgc catttgtggt cgatgatgaa
     6961 tgggaagacg atatcggtct ggtgcataac caacgtattg ttcagtcagg tcagagctgg
     7021 cagcaagggc ctatcgtttt gaactggttg gatatacaag attcttttga tgcatctggt
     7081 tttaacctga ttattcatga agtcgctcat aagctggaca cccgtaacgg cgatcgcgcc
     7141 agcggagttc cctttattcc gttgcgtgag gttgctggct gggaacacga tcttcatgct
     7201 gcaatgaaca acattcagga agaaatcgaa ttggttggcg agaatgcggc gagcattgat
     7261 gcttatgcag ccagcgatcc tgctgaatgt tttgccgtac tttctgaata tttctttagc
     7321 gccccagaac tttttgctcc tcgtttccct tcattgtggc aacgtttctg ccaattttat
     7381 caacaagatc ctttgcagag actgcatcac gctaatgata cagactcgtt ttcggcgacg
     7441 aatgttcatt aattaacaac tttgcagatt aattaaccaa ttgaaatgac ttatgaaatt
     7501 tagtgttgac agacaaggta ccgctaagta atatgtgccc cgttcacacg attcctctgt
     7561 agttcagtcg gtagaacggc ggactgttaa tccgtatgtc actggttcga gtccagtcag
     7621 aggagccaaa ttctaaaaat tcgctttttt agcgcaatgt cactgacctt agttgaacat
     7681 tgttttttaa cggatagcgg gtttttaaca ccttaagcgc cctcgacctt tatggttgag
     7741 ggcgttttgc tatgaacgcc atcaccattt tcccctcgat tataaaactt gagttattca
     7801 gtagtctccc ctcttgcaac tcacacccaa aactgcctaa cgaaaagtta ttaattttca
     7861 atcgtattgc tatcagtatt tacatttttt cgctgtgcta gaaagggcgc atttatgtta
     7921 gctcgttcag ggaaggtaag catggctacg aagaagagaa gtggagaaga aataaatgac
     7981 cgacaaatat tatgcgggat gggaattaaa ctacgccgct taactgcggg tatctgtctg
     8041 ataactcaac ttgcgttccc tatggctgcg gcagcacaag gtgtggtaaa cgccgcaacc
     8101 caacaaccag ttcctgcaca aattgccatt gcaaatgcca atacggtgcc ctacaccctt
     8161 ggagcgctgg aatcggccca aagcgttgcc gaacgtttcg gtatttcggt ggctgagtta
     8221 cgcaaactca accagtttcg tacgtttgct cgaggttttg ataatgtccg ccagggtgat
     8281 gaactggatg tcccggcaca agttagtgaa aaaaaattaa ccccgccgcc gggtaatagc
     8341 agtgacaacc tcgagcaaca gatagccagt acttcacagc aaatcgggtc tctgctcgcc
     8401 gaagatatga acagcgagca agcggcaaat atggcgcgtg gatgggcctc ttctcaggct
     8461 tcaggcgcaa tgacagactg gttaagccgc ttcggtaccg caagaatcac gctgggcgtg
     8521 gatgaagatt ttagcctgaa gaactcccag ttcgattttc tccatccgtg gtatgaaacg
     8581 cctgataatc tctttttcag tcagcatact ctccatcgta ctgacgagcg tacgcagatt
     8641 aacaacggct taggttggcg tcatttcact cccacatgga tgtcgggcat caacttcttt
     8701 ttcgaccacg atcttagccg ttaccactcc cgcgtcggca ttggcgcgga gtactggcgc
     8761 gactatctaa aattaagcag taacggctat ttgcgactga ccaactggcg cagcgcacct
     8821 gaactggaca acgattataa agcacgcccg gccaatggct gggatgtacg cgcagaaagc
     8881 tggctacccg cctggccgca ccttggcggt aaactggtct atgaacagta ttatggcgat
     8941 taagtggccc tgttcgataa agacgatcgg caaagtaatc ctcatgccat aaccgctgga
     9001 cttaactata cccccttccc gctgatgacc ttcagcgcgg agcaacgcca gggtaaacag
     9061 ggcgaaaatg acacccgttt tgccgtcgat tttacctggc aacctggcag cgcaatgcag
     9121 aaacagcttg acccgaatga agtcgctgca cggcgtagcc ttgcaggcag ccgttatgat
     9181 ctggtggatc gcaacaacaa tatcgttctg gaatatcgca aaaaagaact ggttcgccta
     9241 accctgacag accccgtgac agggaagtca ggagaagtga aatcactggt ttcgtcgcta
     9301 caaaccaaat atgccctgaa aggctataac gtcgaagcca ccgcactgga agctgccggt
     9361 ggcaaagtgg tcacaacggg taaagatatt ctagttaccc tgccggctta ccggttcacc
     9421 agtacgccag aaaccgataa cacctggccg attgaagtca ccgccgaaga tgtcaaaggc
     9481 aatttgtcga atcgtgaaca gagcatggtg gtcgttcagg cacctacgct aagccagaaa
     9541 gattcctcgg tatcgttaag tacccaaaca ttgaacgcgg attcccattc aaccgccaca
     9601 ctgactttta ttgcgcatga tgcagcaggt aatcctgttg tcgggctggt gctctcgacg
     9661 cgtcacgaag gtgttcagga catcaccctt tctgactgga aagataatgg tgacggaagc
     9721 tatacccaga tcctgaccac aggagcgatg tctggcacgc tgacgctgat gccacagctg
     9781 aatggtgtgg atgcggctaa agcccccgcc gtggtgaata tcatttctgt ttcgtcatcc
     9841 cgaactcact cgtcaattaa gattgataag gaccgttatc tctccggcaa tcctatcgag
     9901 gtgacggtag aactgagaga tgaaaatgac aaacctgtta aggaacaaaa acagcaactg
     9961 aataacgcag tcagcatcga caacgtgaaa ccaggagtca ctacagactg gaaagaaacc
    10021 gcagatggcg tctataaggc gacctatacc gcctatacca aaggcagtgg acttactgcg
    10081 aagctattgg aatgaagatt tgcataccgc tggatttatc atcgacgcca acccgcagtc
    10141 agcgaaaatt gcgacattat ctgccagcaa taatggtgta ctcgccaatg agaatgcagc
    10201 aaacaccgtc tcggtcaatg tcgctgatga aggaagcaac ccaatcaatg atcataccgt
    10261 cacgtttgcg gtattaagcg gatcggcaac ttccttcaac aatcaaaaca ccgcaaaaac
    10321 ggatgttaat ggtctggcga cttttgatct gaaaagtagt aagcaggaag acaacacggt
    10381 tgaagtcacc cttgaaaatg gcgtgaaaca aacgttaatc gtcagttttg tcggcgactc
    10441 gagtaccgcg caggttgatc tgcagaagtc gaaaaatgaa gtggttgctg acggcaatga
    10501 cagcgccaca atgaccgcga ccgtccggga tgcaaaaggc aacctgctca atgacgtcat
    10561 ggtcactttc aatgttaatt cagcagaggc gaaactgagc caaaccgaag tgaatagcca
    10621 cgacgggatc gccacagcta cgctgaccag tttgaaaaat ggtgattata gggttacggc
    10681 ctctgtgagc tctggttccc aggctaatca acaggtgaat tttatcggtg atcaaagtac
    10741 tgctgccctg accctcagtg tgccttcagg tgatatcacc gtcaccaaca cagctccgca
    10801 acatatgact gcaaccttgc aggataaaaa tggcaaccca ctaaaagata aagaaatcac
    10861 cttctctgtg ccaaacgacg tcgcaagtaa gttctcgatt agcaacggag gaaaaggcat
    10921 gacggatagt aacggggttg caatcgcctc cctgaccggc acgttagcgg gcacgcatat
    10981 gatcatggct cgtctggcta acagcaatgt cagcgatgca cagccaatga cgtttgtggc
    11041 ggataaagac agagcggttg tcgttttgca aacatcgaaa gcggaaatca ttgggaatgg
    11101 cgtggatgag acaactctga cagcaacagt gaaagatccg tcgaatcatc cggtggcggg
    11161 gataacggta aacttcacca tgccacagga cgttgcggca aactttaccc ttgaaaataa
    11221 cggtattgcc atcactcagg ccaatgggga agcgcatgtc acgctgaaag gtaaaaaagc
    11281 gggcacgcat acggttaccg caacgctggg taataacaat accagtgatt cgcagccggt
    11341 aacatttgtg gcggacaaag cctcggctca ggttgtcctg cagatatcaa aagatgagat
    11401 cacaggtaat ggcgtcgata gcgcaacgct aactgcaacg gttaaagatc agttcgacaa
    11461 tgaggtgaat aatcttccgg taacattcag ctcagcctct tcaggactca ccctgacccc
    11521 gggagtaagt aataccaatg agtctggcat cgcgcaggcc actctcgcag gcgttgcctt
    11581 tggtgagcag acggtcactg catcactggc taataatggt gccagcgaca acaaaactgt
    11641 gcattttatt ggcgacacag cggcggcaaa aattatcgag ttgacgcctg tcccagacag
    11701 cataatcgcc ggtaccccgc agaacagctc cggcagcgcc atcaccgcca cagtcgttga
    11761 taataatggc tttccggtga aaggtgtgac tgtgaacttc accagcagaa caaactctgc
    11821 cgaaatgacg aatggcggcc aagccgtaac gaacgaacag ggtaaggcta ccgtcactta
    11881 taccaatacc cgctcctcga tagaatcagg agcgagaccg gataccgttg aggccagtct
    11941 ggaaaatggt agctccacgc ttagcacatc aattaatgtc aacgctgatg cgtctacggc
    12001 acatctcacc tggctacagg cactttttga tacagtctcc gcaggcgaga caaccagtct
    12061 gtatattgag gtgaaggata attacggcaa cggtgtcccc cagcatcagg tgaccctcag
    12121 cgtttcacca agtgaaggcg tgacccccag taataacgct atatatacta ccaaccacga
    12181 cggcaatttt tacgcaagct ttaccgctac aaaagccggg gtttatcaag tgacggcaac
    12241 cctcgaaaat ggcgattcga tgcaacaaac agtgacctat gtgccgaacg tcacgaatgc
    12301 cgaaatcacg ctggcaacct cgaaggatcc ggtgattgcc gacaataacg atctcacgac
    12361 actaacagca acagtcgctg atacagaggg caatgcgata gccaacactg aagtaacatt
    12421 tactctgccg gaagatgtta aggcgaactt cacgctgagc gatggcggta aagcgattac
    12481 tgatgctgaa ggcaaagcga aagtcacgct gaaaggtaca aaagcaggcg ctcatactgt
    12541 tacagcatcg ataactggcg gtaagagtga gcagttggtg gtgaacttta ctgcggatac
    12601 gctcactgcg caggttaatc ttaacgttac cgaggacaat tttatcgcta ataacgtcgg
    12661 gatgaccagg ctgcaggcaa cagtgactga tggaaacggc aacccgttag ccaatgaggc
    12721 ggtgacattc acgctaccgg cagatgtgag cgcaagcttt actctcggac aaggcggttc
    12781 cgccattact gacatcaacg gcaaggctga agttacactg agcggtacaa aatccggcac
    12841 ctaccccgtg acagttagcg tgaacaatta tggtgtcagt gatacgaaac aggtgacttt
    12901 gattgccgat gctggtaccg caacactagc ctccttaacc tctgtatact cattcgtcgt
    12961 cagcacgacc gaaggcgcga ccatgactgc aagcgtcact gacgctaacg gcaacccggt
    13021 agaaggcata aaagttaatt tccgcggaac ctccgtcacg ctaagcagca ccagcgttga
    13081 aacggatgat cggggtttca ctgaaattct tgtgacaagc accgaagtcg gactgaaaac
    13141 agtttcagcc tctctggcag ataaacctac tgaagtcata tcgcgattac tgaatgcaaa
    13201 agcagatatt aattctgcaa cgattaccag tctggagata cctgaaggtc aggtcatggt
    13261 cgcacaagac gtagcagtta aagctcacgt caacgaccag tttggcaacc cgattcttaa
    13321 tgaatctgta acattcagtg cagagccacc agagcacatg accatcagcc aaaatattgt
    13381 ctctactgat acgcatggta tagccgaggt ctccatgacg cccgaaagaa acggttcgta
    13441 tatggtgaaa gcatccctgg cgaatggagc ctcacttgag aaacaactgg aggctattga
    13501 tgaaaaactg acactctcgg cgtccagccc gcttatcggt gtcaattccc caacaggtgc
    13561 aactctgacg gcaacgctaa cttctgcaaa tggcactcca gtggagggtc aggtcatcaa
    13621 ctttagcgta acgccagaag gtgcgacgtt aagtggcgga aaagtgagaa ctaactcttc
    13681 tggtcaggct ccagtcgttc tgaccagcaa taaagtcggt acatatacgg tgactgcatc
    13741 gttccataac ggcgtaacaa tacagacaca gacaaccgtg aaagtcactg gcaactcaag
    13801 caccgcccat gttactagct ttatcgctga tccatcgact atcgccgcca ccaacagtga
    13861 tttaagtacc ttaaaggcaa cggttgagga tg
//
LOCUS       DAJVQG010000110        13804 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJVQG010000110 DAJVQG010000000
VERSION     DAJVQG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13804
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.110"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..730)
                     /gene="dsbG"
                     /locus_tag="OQM83_002891"
     CDS             complement(<1..730)
                     /gene="dsbG"
                     /locus_tag="OQM83_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCS1521100.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWILDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQVKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNI"
     gene            1102..1665
                     /gene="ahpC"
                     /locus_tag="OQM83_002892"
     CDS             1102..1665
                     /gene="ahpC"
                     /locus_tag="OQM83_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCS1521101.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1836..3401
                     /gene="ahpF"
                     /locus_tag="OQM83_002893"
     CDS             1836..3401
                     /gene="ahpF"
                     /locus_tag="OQM83_002893"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCS1521102.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3522..3950)
                     /gene="uspG"
                     /locus_tag="OQM83_002894"
     CDS             complement(3522..3950)
                     /gene="uspG"
                     /locus_tag="OQM83_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCS1521103.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4171..5409
                     /locus_tag="OQM83_002895"
     CDS             4171..5409
                     /locus_tag="OQM83_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCS1521104.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDILGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5640..6050)
                     /gene="rnk"
                     /locus_tag="OQM83_002896"
     CDS             complement(5640..6050)
                     /gene="rnk"
                     /locus_tag="OQM83_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCS1521105.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6280..7086)
                     /gene="rna"
                     /locus_tag="OQM83_002897"
     CDS             complement(6280..7086)
                     /gene="rna"
                     /locus_tag="OQM83_002897"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCS1521106.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKNFVIDKVGY"
     gene            complement(7200..8663)
                     /gene="citT"
                     /locus_tag="OQM83_002898"
     CDS             complement(7200..8663)
                     /gene="citT"
                     /locus_tag="OQM83_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCS1521107.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8714..9592)
                     /gene="citG"
                     /locus_tag="OQM83_002899"
     CDS             complement(8714..9592)
                     /gene="citG"
                     /locus_tag="OQM83_002899"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCS1521108.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9567..10118)
                     /gene="citX"
                     /locus_tag="OQM83_002900"
     CDS             complement(9567..10118)
                     /gene="citX"
                     /locus_tag="OQM83_002900"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCS1521109.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10122..11654)
                     /gene="citF"
                     /locus_tag="OQM83_002901"
     CDS             complement(10122..11654)
                     /gene="citF"
                     /locus_tag="OQM83_002901"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCS1521110.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANQEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            complement(11665..12573)
                     /gene="citE"
                     /locus_tag="OQM83_002902"
     CDS             complement(11665..12573)
                     /gene="citE"
                     /locus_tag="OQM83_002902"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCS1521111.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12570..12866)
                     /gene="citD"
                     /locus_tag="OQM83_002903"
     CDS             complement(12570..12866)
                     /gene="citD"
                     /locus_tag="OQM83_002903"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCS1521112.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12881..>13804)
                     /gene="citC"
                     /locus_tag="OQM83_002904"
     CDS             complement(12881..>13804)
                     /gene="citC"
                     /locus_tag="OQM83_002904"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCS1521113.1"
                     /translation="INKLESLPIAGNIIKCVAISESVRGEGLALTLATELINLAYERH
                     STHLFIYTKTEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKI
                     GCIVMNANPFTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPR
                     LTVHRGSEYIISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEP
                     FCRVTAQYNQDMRYWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAI
                     APLVPAVTLHYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3386 a         3497 c         3674 g         3247 t
ORIGIN      
        1 tgatattaag cgttttctga tcgggcaacc ccacggcctg ttgcagcgta ttttccttac
       61 tcatgtaata gatagccggc gtgacatttg cccccagatc gtccatcagt ttctcattgt
      121 cacttaacac tttcacttgc tctgtactta cgtttgcagg cacgtttagc ttaagcttgc
      181 caccagaggc ttcatattgt tgccaggttt ttgcgggatc tttggaggca agaattgccg
      241 ctgctgtcgc cgggctttct ggcttgataa ccccaaccaa caatgttctt aattgcactt
      301 tgccagaatc tacccacggg cgcgcctgct gccagaactg tttacaatat gggcagaacg
      361 gatcggcgaa gacgtagaca atgaccggcg catctttttt accgtcgagg atccagtggg
      421 attgttccat ccgttgccac atttcgcgtc cggctggtgc gtaaatttct ttttcgataa
      481 gtgtgttact caggttttca cctttctcgt tgtacatgta accagagata gcgtgcttac
      541 catcgggagt caggtagatg gtgacgccca tatcctgata ctttccgaga taacctttca
      601 ttcctccggg ggcatcgaac gttttgatga ttgtaatgcc ctgtttttca atcgctttta
      661 ctggggaagg aagttcctct gcgaaggcga ttgcaggaag cagagccagt aaaagtatct
      721 tttttaacat taatttgtcc ttttcagtca gtgcaaaagt cgagtaaaag gcataaccta
      781 tcactgtcat aggtaagagc ttagatcagg tgattgccct ttgtttatga gggtgttgta
      841 atccatgtcg ttgttgcatt tgtaagggca acacctcagc ctgcaggcag gcgctgaaga
      901 taccaaaggg tagttcagat tacacggtca cctggaaagg gggccatttt actttttatc
      961 gccgctggcg gtgcaaagtt cacaaagttg tcttacgaag gttgtaaggt aaaacttatc
     1021 gatttgataa tggaaacgca ttagccgaat cggcaaaaat tggttacctt acatctcatc
     1081 gaaaacacgg aggaagtata gatgtccttg attaacacca aaattaaacc ttttaaaaac
     1141 caggcattca aaaacggcga attcatcgaa atcaccgaaa aagataccga aggccgctgg
     1201 agcgtcttct tcttctaccc ggctgacttt actttcgtat gcccgaccga actgggtgac
     1261 gttgctgacc actacgaaga actgcagaaa ctgggcgtag acgtatacgc agtatctacc
     1321 gatactcact tcacccacaa agcatggcac agcagctctg aaaccatcgc taaaatcaaa
     1381 tatgcgatga tcggcgaccc gactggcgcc ctgacccgta acttcgacaa catgcgtgaa
     1441 gatgaaggtc tggctgaccg tgcgaccttc gttgttgacc cgcagggtat catccaggca
     1501 atcgaagtta ccgctgaagg cattggccgt gacgcgtctg atctgctgcg taaaatcaaa
     1561 gcagctcagt acgtagcttc tcacccaggt gaagtttgcc cggctaaatg gaaagaaggt
     1621 gaagcaactc tggctccgtc tctggacctg gttggtaaaa tctaaatttc cttcgtcttt
     1681 cgcgctacca gctccggcgc gaaatacttc ggaaattcac ctaattcttc gggtgctgcg
     1741 gcacccgatt ttcttccccg caccatgatg caagctgcat ccaggtagcc gtagaggccg
     1801 cttgcatgat gatgtttaaa gcccaggaga taaacatgct cgacacaaat atgaaaactc
     1861 aactcaaggc ttaccttgag aaattgacca agcctgttga gttaattgcc acgctggatg
     1921 acagcgctaa atcggcagaa atcaaggaac tgttggctga aatcgcagaa ctgtcagaca
     1981 aagtcacctt taaagaagat aacagcttgc cggtgcgtaa gccgtctttc ctgatcacca
     2041 acccaggttc caaccagggg ccacgttttg caggctcccc gctgggccac gagttcacct
     2101 cgctggtact ggcgttgctg tggaccggtg gtcatccgtc gaaagaagcg cagtctctgc
     2161 tggagcagat tcgccatatt gacggtgatt ttgaattcga aacctattac tcgctctctt
     2221 gccataactg cccggacgtg gtgcaggcgc tgaacctgat gagcgtactg aacccgcgca
     2281 tcaagcacac tgcaattgac ggcggcacct tccagaacga aatcaccgat cgcaacgtga
     2341 tgggcgttcc ggcagtgttc gtaaacggga aagagtttgg tcagggccgc atgacgttga
     2401 ctgaaatcgt tgccaaaatt gatacgggcg cggaaaaacg tgcggcagaa gagctgaaca
     2461 agcgtgatgc ttatgacgta ttaatcgtcg gttccggccc ggcgggtgca gcggcagcaa
     2521 tttactccgc acgtaaaggc atccgtaccg gtctgatggg cgaacgtttt ggtggtcaga
     2581 tcctcgatac cgttgatatc gaaaactaca tttctgtacc gaagactgaa gggcagaagc
     2641 tggcaggcgc actgaaagtt cacgttgatg aatacgacgt tgatgtgatc gacagccaga
     2701 gcgccagcaa actgatccca gcagcagttg aaggtggtct gcatcagatt gaaacagctt
     2761 ctggcgcggt actgaaagca cgcagcatta tcgtggcgac cggtgcaaaa tggcgcaaca
     2821 tgaacgttcc gggcgaagat cagtatcgca ccaaaggcgt gacctactgc ccgcactgcg
     2881 acggcccgct gtttaaaggt aaacgcgtag cggttatcgg cggcggtaac tccggcgtgg
     2941 aagcggcaat tgacctggcg ggtatcgttg agcacgtaac gctgctggaa tttgcgccag
     3001 aaatgaaagc cgaccaggtt ctgcaggaca aactgcgcag cctgaaaaac gtcgacatta
     3061 ttctgaatgc gcaaaccacg gaagtgaaag gcgacggcag caaagtcgtt ggtctggaat
     3121 atcgagatcg tgtcagcggc gatattcaca acatcgaact ggccggtatt ttcgtgcaga
     3181 ttggtctgct gccgaacacc aactggctcg aaggcgcagt cgaacgtaac cgcatgggtg
     3241 agattatcat tgatgcgaaa tgcgaaacca acgtcaaagg cgtattcgca gcgggtgact
     3301 gtacgacggt tccgtacaag cagatcatca tcgccactgg cgaaggtgcc aaagcctctt
     3361 tgagtgcttt tgactacctg attcgcacca aaactgcata agaagaagta agattcacct
     3421 gcaattgctt agccgccggg gtcaaacctg gcggcttttt tatggcatta aaaagccctg
     3481 ccgggatgtt ccaggcaggg cggcataaat acttttatct gttagcgaat gaccagtaca
     3541 ggcagattgg cgtggcggat cacgctcgag gcgttagaac ctaacagatg ggtcgaaatc
     3601 gatgggttgc gagaaccgat aactacaaca tcagccccca gttcttctgc caactcattg
     3661 acttcatccc gcacgctacc aaaacggaca tgttgtttaa tgcgggaagg atcgatggtg
     3721 aagtggctga ccatcgtttg cagacgttct tctgcttcat gttgcagatg ctcttcaaaa
     3781 cgacgcacat cagcggcaaa acggtgcagg ctcaggctgg ctgacccggg tagtacgtga
     3841 agtagatgaa taactccgtc atcctgggcg aggaattcag cgtggcgaac agctttgtcg
     3901 ctcaattcca tttcaaatac atcaactggc ataatgattg tcttatacat aaccctttct
     3961 ccctgttaat catgaacaaa tcattcgcca tgattataat atttatccct gatatttgtc
     4021 tggttctttt tccttacgaa cggtttctgt gattaatata ttctcactga acaccaggaa
     4081 ttctcccaaa acctgcggta ccgcccgttt tcccgctgtg atagctaccc ttaaagactg
     4141 actctttttt gaactgtctc tggaggttgc atgaaagcat tgacttatca cggcccacat
     4201 cacgttcagg tagaaaatgt tcccgatccg ggcattgaac aggctgatga tattattctg
     4261 cgtattacgg caacggcgat ctgtggctct gacctccatc tttatcgagg caaaataccc
     4321 caggttaaac atggcgatat tttgggtcat gaatttatgg gggaagtcgt tgaaaccgga
     4381 aaggacgtaa aaaatttgca aaaaggcgac cgggtggtaa ttccgtttgt cattgcttgt
     4441 ggcgactgtt ttttctgtcg aatgcagcaa tatgccgcct gcgaaaatac caatgcgggt
     4501 aaaggcgctg cgctcaataa aaaacagata ccagctcccg cggcattgtt tggttatagt
     4561 cacctgtatg gtggcgttcc tggtggacag gcggaatatg tccgcgtccc taaagggaat
     4621 gtggggccgt ttaaagtacc gcctttgctt tcagatgata aagccctttt cctttctgat
     4681 attctgccaa cggcatggca ggcagcaaaa aatgcgcaga tccaacaagg ttcaagcgtt
     4741 gcagtctatg gtgccggtcc agtgggattg ttgacaatcg cctgtgcacg gttgctcggt
     4801 gcggaacaga tttttgttgt tgatcatcat ccctaccgct tgcgtttcgc cgctgaccgc
     4861 tacggcgcga tcccgattaa tttcgatgaa gatagcgatc cggcacagtc aattattgaa
     4921 caaacggcag gtcaccgggg cgtggatgca gtaatagacg ccgtcggttt tgaagcgaaa
     4981 ggcagcacca cggaaacggt gctgactaac ctgaaactgg agggcagcag cggtaaagcg
     5041 ttgcgccagt gtattgcggc ggtcaggcgt ggcggcattg ttagcgtacc gggcgtctac
     5101 gctggattta ttcacggttt cctgtttggc gacgcctttg ataaagggtt gacgtttaaa
     5161 atgggacaga cccacgttca cgcatggctg ggtgaattat taccgttaat tgagaaagga
     5221 ttactgaaac cagaagaaat tgttacccac tatatgccgt ttgaagaggc cgcccgggga
     5281 tatgagattt tcgaaaaacg tgaagaggag tgccgtaagg tgattctggt acccggtgca
     5341 caaagcgcag aggcggcgca gaaggcggtt tcaggtctgg tgaatgcgat gccgggggga
     5401 acaatatgat cgtcaggagt ggttttcgag gtaaaggaca gccatgacga taatcgccgc
     5461 cataatcaga aatcctatca ggatgtaaaa tgcttctgcc atggttattc ccacaaacga
     5521 aacggaataa ttttgcagca aaagtgaaca gtgagaacca ggaaaaattg ctgattttgc
     5581 gtaaagagga tgcgagtgca tcctctgggc aaagcgagtt atcgcttgtg cagatgggat
     5641 taaagcaggt agtcgccagc agcttctggc tggtattcga gttccagcac ttcaaggtgg
     5701 gttgcaacgc cgcccggaag ttcccagtga atggaatcgc caacgcgcag ccccagcagt
     5761 gcggcaccta ccggagccat aacggaaagc tgagtattgc tatcggtcat ttttgccgga
     5821 tacaccagcg tgcgcacacg cacttcgcca tcgctaagat tgcggaattt aacccggctg
     5881 ttcattgtca ccacgtcgtg tggcatctct tctggcgaac acatttgggc gcgatccaac
     5941 tctgcgttta acgcgtcggc gattggcaaa ccagcatagg cgggttgctc cagcagaata
     6001 tcgatgcgtt cggcatccag gtcgttaatg atgatagttg gtctggacat ttttactcca
     6061 tgtcgtcggt actgcgagtg tcgcagataa acatacccaa aagaaaaccc tcaccgtcag
     6121 gcggcgaggg tttaactcac atgatgatac tgactgttgc tcactctttg aagtgatttg
     6181 cgtcacattc agggaattcc tcaatgcagc gcattatgta taattcttaa tcgtcacggt
     6241 ttatggaaga cgaattagcg tgttttgtaa atcagatgat taataaccca ctttatcaat
     6301 cacaaagttt ttgccacagt tgcctgggtg tggttgtggc aagaatgagt ttgcagaaag
     6361 cggagcgttg atggcgtcag ctttgatcga gatctgaatt tcagtcaaat acgcagggtt
     6421 accctggcac gttagcttaa ctgctttcac gttctctttt ccccagcttt tggcaaaggc
     6481 ggcgtcaaag tcacggcggc tcactgtttt accgtaatta tccgcaagga attttccggc
     6541 ttcgctctct ttaatttctt gattcaggcg taccatcgta ccgaagtatg cgtccggatc
     6601 aaaaccaaag caggcaccgt gtttggcata ttcgtagcgt tccaggcagg aacgtccgcc
     6661 agctcctggc atgacttcac ttagtttagc ggccgtttcc agtgataacc cggtttccgg
     6721 cgatgaacac atccggctgg cgtgcgcttc tggtagattc gggattgggc gagtagcgca
     6781 accgaagcgc atccagcggc gttcatcaac accacgggca gcaaccgatt taggcaatcc
     6841 tggccacaga ccatgtacgg tcagaaaatc agctttgttg gtcgtttcgg tttgtaggcg
     6901 acattcatct cgttcgttac gatttcgatc gtgttgactc tggcaaaatc cggtttgcca
     6961 ggagagggcc aggacatagc gatcaaaatc gccatactgt tttgcctgca acgctacggc
     7021 gttggcagaa gagaagggaa gcagagaaac cgcgagcaac gcggcgttac gccagaatgc
     7081 tttcataatg ggtgtggaac tcatacatac actgaatact atctattaaa tcataaaaaa
     7141 gcccgccagg tgggcttacc tggcgggcgt gatgatttat tcagcgtttg gcgaacgtat
     7201 tagttccaca tggcgagaat cggccagcca accaacagca acatagagat gtaaatcacc
     7261 ccgaagattg cgccaagacg ccagtaatct tttgatttca catagccaca gccgtaaata
     7321 atcaccccag gaccggttgc atacggcgtc agacagccca tgataccgat agacagcacc
     7381 agcaggatac acagttgttc cattggtacg cccggaatac ctttaccgac ggccagaata
     7441 accgacagca tggttgcggt gtgcgcagac aggctggcaa acaggtagtg tgcaaagtag
     7501 aacaccagaa ccagtacaat caccgttgcg tttggtgaga atccttccag gtgcgtactc
     7561 atggtaccgg cgaaccagtc aataaagcca gaacgagtca ggccgttagc catcacaacc
     7621 agagttgcca ggttgaccag cgtgttccat gcgctgttat agcgggtaat atctttccaa
     7681 ggcacaacgt gcagggccag cattagcgaa actgccagca gaccaactgc agtagcatta
     7741 atgacttcac tgccaaaaac ccacaaacct aagctgagca atacaaggcc aatcagtgtc
     7801 cactctctgc gtgtcagcgc acccatggtt ttcagttcat cacccgccca ggttgccact
     7861 tcttcactgt gtgtgatttc cggtttgtac agcacgtagg aaagccacgg cgcaatgata
     7921 agcaagataa ccccaaccgg caggaagcag aggaaccact gcaaccagct aatctggata
     7981 ccggcaattt tgctgacgaa ctccagaccc agcacgtttg gtgccgcacc ggtgacaaac
     8041 atggacgaac tcagactggt actaatgacc atcatccaca tcaaatagcc gccaatacga
     8101 cgcgcggacg gatcgttggg gaatgattta aacagcggcg gcaggttttt aatgaccgga
     8161 aaaaccgtac ccccggtacg cgcggtgttg gacggtgtaa acggtgccag cagaatgtcg
     8221 ataatgacaa tcgcataacc caacgtcagc gtgcgtttgc ccatgaattt caccaggaaa
     8281 agggcaatgc gacgacctaa cccggaaact tcatacccta atgcaaaaat aaatgcgcca
     8341 aataccagcc ataccgtggt gctggaaaaa ccagccagac cccatttcag cgcctgtttt
     8401 tgcgcattaa acgctgggtc agctaattct ttggcatcaa agagcaggta attactgcca
     8461 ataacgcaaa tagtaaccgc aataaaactg atcgctgttg ccggaattgg ctcgaggatc
     8521 atgccgacaa tcattgccac aaacacagcg aagtaatgcc atgcctgtgg tggcataccg
     8581 tcggggacag ggataagaaa catgaccccc atcaccacca gtggggccaa tagtttccat
     8641 atattatctt ttgctaaaga catacgggtt ctccgaaaat taatatttcc aaatttatca
     8701 agtgcttaaa taattaaatc tgtgctaaaa accaggtaag gatcagcagg tcggcactgc
     8761 cgcctggact gagatttcgt tcgatacact ccctgtcgaa ctgccggaga taatcgagat
     8821 cggcgggggt tcgaatgccc cctttttgca ataatgtttg cgcctcgcgc tgtagccagc
     8881 gcaggccccc ctcgccaccg cgcgatgcaa cgttggtatc gccgttgatc gccatcagta
     8941 ggagcaaggt atcgagcaat gccagttcag gatctaaccc ctgatccagc agagtgaggt
     9001 aatgcggcaa ggcgtgattg atcaccagtg gataacccgc ttcggcttca ccgcgtgcgc
     9061 cggtaaggcc aagctgctgg tacaaccgtt gacctgccgt cagttgtgaa ttattggtac
     9121 gcagttctcg atcggtcagg ccacggcaga aacttgccgc cgtagaacaa acggttgttg
     9181 gcgttaccgg ttggttgagt tgaagcaaac ggccaattgc cgcacatagt agccctaaag
     9241 aaaaaatgct gcctttatgc gtgtttacgc ccgcagtggc gcggaacata tcaccttcgc
     9301 aagccatacc aattgggcgt aatccgtgga gtaccgcttc tggtgctatt tccgcactac
     9361 aggcaccaaa ttcaatgaaa cggggtagcc agccctgaat cgccagcgcg ctgcggtgga
     9421 aatcttccag cgccatatct ttatgtgcac cgcagttaat ccgatccacg aggcctggtt
     9481 tcggtgacag attgacttca gtcagcatgg cgcgccagcc cagcagggcg tactcatcga
     9541 tcaatgacgt cgcaagcttt gtggttttag ttgacgttgc aggcatcgac atcgttcagc
     9601 agtgcctcca tgcggttgag taaatcggtc agttgatggg tttttccacg cgcgcagacg
     9661 gctgcgcttt gctcgcacaa caggcagcgg cgaggcggca gtgaatagtc gcggcgggag
     9721 agaatttcgc cttcgggcgt caggacatcg atatcccata accgcccgag aggatgacta
     9781 tgttcaagct caatggtggc gagcttgagg tcgcgagccg gggcggcaat gctcaacatg
     9841 ccctccggcc cgctggcgga aaccagtgca gcctgctcct gaatttgcca gccctgtttt
     9901 gcggctaagg cacgcaaggc tgtcacgcca tgattaaaaa ttcggcgtgt gacctcgctg
     9961 tctttaatcg gcccaggcgc aaccacggta aaggagacca gtggaacagg atggcgcttg
    10021 agccagacgt gttgccgtgc ttgcctttca tcccggctga cgagcagctc gggaattgac
    10081 accgcatggt ggctggcgag ttcaggaagc aggtgcatgg cttattcctt cacctgatgc
    10141 acaacatcga tcaccgagcc atcgcggtaa cgcacaacgg caacgacgcg gtcggtgaat
    10201 tcaatcggct gtggttcacc ggtcagcaga cgcgcgcgtt cgcgcagcca ctcaatggaa
    10261 accactttaa tgcccgcttc ctgcagacgt tctgccagtt ccggacgtgc cgggttaact
    10321 gcgataccgt ggtctgtgac cagaatatcg acactggagc ctggggtgat gcaggtcagc
    10381 acgttatcca ccagagtcgg aatacgaccg cgtaccagcg gcgcgacgat gatggaaagc
    10441 gcagaggcaa tcgcggtatc gcagtgacca ccggaagcac cacgcagtac gccgtcagag
    10501 ccggtcagca cgttaacgtt gaactgggtg tcaatttcca gcgcgctcag taccaccacg
    10561 tcgagacgat caaccgatgc gcctttcgaa ccccagttag cgtactggtt ggcgctgatt
    10621 tcgatgtgat tggggttacg ggccagcgat tgcgcagcat ggctgtcaaa gctctgcaca
    10681 tccagcagtt tgcggatcag acctttttcg tgcagatcaa ccatcgtcgc ggtaataccg
    10741 ccgagggcga agtcggcgcg aatatcgcgg ctacgcattt tgtcttccag gaaacgggtt
    10801 accgccagcg atgcgccgcc ggtgccggtt tgcatggaga aaccttcttt gaagtagcca
    10861 gagttgacaa tcacatccgc agcgctacgg gcaataagca gttcgcgcgg gttagtggtc
    10921 atacgggtcg cgccagcgcc gatttttgca gcatcgccaa cgcggtcaac tttgacgatc
    10981 aaatcaacct gatcttgctc aatgcttgcc ggattatgcg gataaggcag cagttcttcg
    11041 gtaagcatca caacctgttt tgcgttgtcg gcatcaacca ttgcatagcc gagggagccg
    11101 cagcaggctt taccggtgta accgttggca ttaccgaatt catcacagga cgggacgccg
    11161 aggaaagcca cgtcgatatt cagttcgccg ctctgtacca gatgcacacg tccgccgtga
    11221 gagtggattt gcaccggttc cgcaagcaga ccacgggaaa tttcttctgc cagtggaccg
    11281 cgcaggccgg aggtataaat gcgggtaacc acgccctggc gaatgtgttc taccagtggc
    11341 gcatggcaat cactcaggga gctggacgcc agggtcaggt ttttaaagcc catcttagcg
    11401 atgacgtcca tcaccatatt gacggtcagg tcaccgccac ggaaagcgtg atggaaggaa
    11461 accgtcatgc cgtcctgtaa accagagcga cgaatcgctt cttcctggtt ggcgcacagt
    11521 ttgcgatcgc gcgctttttc agcctggtag gtttgctttg gtgagttctg gaaagcggca
    11581 agatcgcatt cagcgcgacg attccaggcc gctacccgtt cttgtcgttg agattgttca
    11641 attttctgcg tcattttgat tgccttattc ttcgcggatg ccggaaagtt ctgcacggga
    11701 gagcaccaga cgggcacgat cgataaccgg accgtccacc atcttgccgt tcagggaaac
    11761 cacgccgagg ccttcgcgag cggcggcttc agcggcttct acgacgcggc gggcgtgatc
    11821 cacttctttc tgggtcggtg cgtagaggtt gtgcaacaga tcaatctgac gcgggttgat
    11881 cagcgatttg ccgtcaaagc ccagctgttt gatgtgggcg gcttcttgca ggaatccggc
    11941 ttcgttgtta gcgtcggaat agacggtatc gaacgcctga atacccgcag agcgcgcggc
    12001 ctgcaaaatg gaacagcgtg cgaacagcag ttcagttcct tccggggagc gttctgtacg
    12061 caggttgcgc acatagtctt ctgcaccgag ggcgataccg atcaaacgct cggaagcgtg
    12121 agcgatttcc actgcgcggg taatccccag cggagattca atcgccgcca gcaggccggt
    12181 gctgccgggt tcacgaccac aggctttttc gatacgcagg atctcttttt cgatatccag
    12241 aacatcctga gcggtatcgg ttttcggcag acgcacaacg tccgcaccac cgcgaacgac
    12301 ggcttccagg tcgttgacac cccattcgga atccagcgcg ttgacacgca caatggtttc
    12361 aatatcgcga tacagcggat gttgcagcgc gtggtaaacc atgcggcggg cggtgtcttt
    12421 ttcacgcaat gctacggagt cttcgaggtc aaacatcagg gcatcagccg ggtagatgaa
    12481 ggagttgctg accatcgcgg cattggcacc aggcacaaac aacatgctgc ggcgggtgcg
    12541 agttttacgt tgttgcagcg aagcggaaat cattggcaat cctcccatgg cagagccggg
    12601 ataccgctgg cgcgtgccag cagggcttcc agtcgtgcac gtaaaatgca gtccagtgcg
    12661 cctttgtcat cgacattcag ctgtacgccg cgcacgttgt agcgggcgag aacgtccaga
    12721 atggtggtgc gaattgcatc gccaaactgt ttctcaacgc tgctattgat ttgcaggtcg
    12781 atatcctgcg tatcgagtgg ggcgatgcgt atcatcacat ccccagactc aagggtgcct
    12841 gcaacggcgg gctggtttat tttcattttt cacctgtttc tcatgcgggg gtcttttgac
    12901 gagctgccgc gtcctggcgg gagtgctcaa gcaggttctg caaataatgc agcgtgactg
    12961 cagggaccag cggcgcgata gccgtgagat cgtttttcgc cagcagttga cgtacccggg
    13021 aagcggatat cggcatctcc tggtaacgca gccgctcaat ttcaaccagt tcgatgggcg
    13081 gtgcggagat agtcggcgtt tccagccagt agcgcatatc ctggttgtac tgggcggtaa
    13141 cgcgacaaaa gggttcagta ccgacaaagc ggtgagttac acccagcgcg ggagcgaggt
    13201 actgacggaa aatcttcaga tcaatttcgg tgtaacaatg gttaatgacg ctctgttctt
    13261 taatgaagta gcaagggaac gtagcgcggg agatgatgta ttcggagccg cgatgcacag
    13321 tcaggcgtgg aatatcggcg gtgcctttta acaccagatc caaccggtct tcatagggga
    13381 agcgtgaaga atcttctttg actaaaaaca gatgcaacca gtcgcactgt gccgcagctt
    13441 gttgaatcag ataacggtga ccattcgtga agggattggc gttcatcaca atgcagccaa
    13501 tcttgttccc tggatgacga aattttttca gcgattcggc atagcgtttc agccgcgtgg
    13561 cgctgttttc catcagcacc atcacgccgg gtacgctggt cagcgtggaa aaaccgcact
    13621 ggcggaacag cgcctcgtat tcggttttgg tataaataaa cagatgcgtg ctgtgccgct
    13681 cataggcgag gtttatcaat tcagtggcta atgtcagcgc cagtccttca ccgcgaacgg
    13741 attcactgat agcaacgcat ttaataatat ttccggcaat tggtaatgac tccaacttat
    13801 tgat
//
LOCUS       DAJVQG010000111        13265 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJVQG010000111 DAJVQG010000000
VERSION     DAJVQG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13265
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.111"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(898..1989)
                     /gene="ychF"
                     /locus_tag="OQM83_002905"
     CDS             complement(898..1989)
                     /gene="ychF"
                     /locus_tag="OQM83_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCS1521114.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(2106..2690)
                     /gene="pth"
                     /locus_tag="OQM83_002906"
     CDS             complement(2106..2690)
                     /gene="pth"
                     /locus_tag="OQM83_002906"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCS1521115.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2967..3245
                     /gene="ychH"
                     /locus_tag="OQM83_002907"
     CDS             2967..3245
                     /gene="ychH"
                     /locus_tag="OQM83_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCS1521116.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(3300..4979)
                     /gene="dauA"
                     /locus_tag="OQM83_002908"
     CDS             complement(3300..4979)
                     /gene="dauA"
                     /locus_tag="OQM83_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCS1521117.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(5103..6050)
                     /gene="prs"
                     /locus_tag="OQM83_002909"
     CDS             complement(5103..6050)
                     /gene="prs"
                     /locus_tag="OQM83_002909"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCS1521118.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(6201..7052)
                     /gene="ispE"
                     /locus_tag="OQM83_002910"
     CDS             complement(6201..7052)
                     /gene="ispE"
                     /locus_tag="OQM83_002910"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCS1521119.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(7052..7675)
                     /gene="lolB"
                     /locus_tag="OQM83_002911"
     CDS             complement(7052..7675)
                     /gene="lolB"
                     /locus_tag="OQM83_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCS1521120.1"
                     /translation="MPQPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7889..9145
                     /gene="hemA"
                     /locus_tag="OQM83_002912"
     CDS             7889..9145
                     /gene="hemA"
                     /locus_tag="OQM83_002912"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCS1521121.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            9187..10269
                     /gene="prfA"
                     /locus_tag="OQM83_002913"
     CDS             9187..10269
                     /gene="prfA"
                     /locus_tag="OQM83_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCS1521122.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            10269..11102
                     /gene="prmC"
                     /locus_tag="OQM83_002914"
     CDS             10269..11102
                     /gene="prmC"
                     /locus_tag="OQM83_002914"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCS1521123.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            11099..11491
                     /locus_tag="OQM83_002915"
     CDS             11099..11491
                     /locus_tag="OQM83_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCS1521124.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11495..12303
                     /locus_tag="OQM83_002916"
                     /pseudo
     CDS             11495..12303
                     /locus_tag="OQM83_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
     gene            12339..13193
                     /gene="kdsA"
                     /locus_tag="OQM83_002917"
     CDS             12339..13193
                     /gene="kdsA"
                     /locus_tag="OQM83_002917"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCS1521125.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3346 a         3286 c         3394 g         3239 t
ORIGIN      
        1 tttaattcag cgagacgatc tgctttggta ttcccattgt gttgtttgat gcccatacca
       61 ctcctatata gtacccagac tatgggtcta tggattttgt cattttgttt aaatcaatga
      121 ataatatcct cttatcatca tgatatttgt atggtgcaat catgatgagc ttctcagaga
      181 ataattctct attaccgggc aaaatcagga acacaggaat taatatatat ttaacatccc
      241 ccttacaaag gtgagtttat atcgagttat tccctaagtt gtcgttactt attccaattc
      301 gttactataa atatggcaaa aatattacaa caaacagcgg ttaaaaaatt tggtgaagat
      361 tttttgtaat aatcctaatt agttatggga atttcgcttg atgtatctgc ataacaaaaa
      421 caatatgcgc catttttgca ttactaataa gaaaaagcaa aaaacatcga tattcatttt
      481 tggtcaatgg gttttctggc ctatcgttgt tattgtgcta ctggttatca ttttcatctt
      541 gccaggatcg cacgtaatca cgttattcgg ggactttatc tgcgggatat tacccttcag
      601 gtaagtcctt ttctcgccag catattggag cgtgatcaat attgatcagc tgtgaagggg
      661 gtgatgtgta gttgaacaaa aacttacgtt atatcactac taatgagata tccgtcatct
      721 gacactgatg tctttaatta ttctcgtggt tcactgtagg caataagcac aaaagtgtag
      781 gatgttacaa gaatgattag gactcggtga aatgaaaaat ccacgcaatt gcgtggattt
      841 tatatacttt tgcgctcttc atgagattta gcgaaacctc atgagacaat aaattaatta
      901 gacgttgaaa aggaagttca tcacatcgcc atctttaacg atgtaatctt taccttctgc
      961 acgcattttg cctgcttctt tcgcgccttg ttcacctttg taagtgatga aatcttcaaa
     1021 cgagatggtt tgtgcacgga taaagccttt ttcaaaatca gtatggattt tgcccgctgc
     1081 ctgcggcgcg gttgctccaa ccggaatggt ccatgcacgc acttctttca ccccagcggt
     1141 gaagtaagtt tgcaggttca gcagcttata accgacacgg atcacacggt tcaggcccgg
     1201 ttcttccagc ccaagctcct gcataaactc gtcacgttct tcgtcgtcca gttcggcaat
     1261 gtctgcttca acagcagcac aaaccggaac cacaacagaa ccttctttcg ccgcgatttc
     1321 acgcacctgg tcaagatatg ggttgttttc aaaaccgtct tcgttgacgt tggcgatgta
     1381 cattgttggt ttcagcgtca ggaagctcag gtaacgaata gccgctttct cttcagcgct
     1441 taaatccagc gcgcgcagca tacctgcgtt ttccagctgg ggcaggcatt tttctaggac
     1501 cgccagctca gctttcgcgt ctttatcgcc acctttggct ttcttctgta cgcgatgaat
     1561 cgcacgttcg caggtgtcga ggtctgccag cgccagttcg gtgttgataa cttcaatatc
     1621 gtcagccggg ttaactttgc cggaaacgtg aatgatgttg tcattttcaa agcagcgaac
     1681 aacgtgaccg atcgcttcgg tttcacggat gttggtcagg aactggttac ccagaccttc
     1741 gcctttcgat gcgcctttta ccagaccggc gatatcgaca aattccatgg tcgtgggaag
     1801 cgtacgttgc ggttttacga tttcagccag ttgatccagg cgagggtcag gcattggtac
     1861 gacgcctgtg ttcggctcaa tggtgcagaa tggaaagttg gccgcttcaa taccggcttt
     1921 ggtcagcgcg ttgaacaggg tagatttccc gacgttgggc aaaccgacga taccgcattt
     1981 gaatcccatg atttaaatca ccttaatatc ttaataatca acctgttatt gctaacagat
     2041 tgcagaaatg gaaataactt tgcctattat acacggcact cggcaaaaat gccgcagaca
     2101 acgacttatt gcgctttaaa ggcgtgcaat cggttcgttg ctttggtcaa gccatctgta
     2161 aaccacattt cagtacaacg cgccgcttcg tcgatggctt catcaattaa cttctgttca
     2221 ctaacaggcg gtttgcctaa cacaaaaccg acaactttat ttttatcgcc aggatgaccg
     2281 attccgatgc gtaaacggtg aaagttaggg ttattaccca atttactgat gatgtctttc
     2341 agtccattgt gaccaccatg gccaccgccc aatttaaatt tggcgacgcc aggaggcaga
     2401 tccagttcgt cgtgggccac cagaatttcg tccggattaa tgcggaaaaa actggccatc
     2461 gccgcaacgg ctttgccgct gagattcata aatgtagtcg ggactaacag gcggacatct
     2521 tcgcctccaa gagtgactcg cgaagtataa ccaaagaatt tagcctcttc gcgcagcgga
     2581 gcgcgcaaac gctctgccag taagtcaacg aaccaggcac cagcattatg tcgcgttgcg
     2641 gcgtattcag caccggggtt cgccaggccg acaatcaatt taatcgtcac gttttttgtc
     2701 ctgagtgtgt acataactgg cgcgtagttt actggttgcg gccccgcttg acaaaaaact
     2761 gcgtatcaaa tgcagataac gtaataattg cctgagtgga ctattagaaa gtcaaggtgt
     2821 tcaggcgttt atttgtaaag ttttgttgaa ataagggttg taattgtgat cacgcccgca
     2881 cataacccac agggtgttgt ctatacttta cacataagga agaggggtat tccctgttac
     2941 aacccagaaa gttccggagg tgacatatga aacgcaaaaa cgcttcgtta ctcggtaacg
     3001 tgctcatggg gttggggctg gtggtaatgg tggtcggcgt ggggtattca atcctcaacc
     3061 agttaccaca gtttaatatg ccccagtatt tcgcacatgg tgcagtgcta agtattttcg
     3121 tcggtgccat tctctggctg gcgggtgccc gtgttggcgg gcatgaacag gtgtgcgacc
     3181 gttactggtg ggttcgtcac tatgacaaac gttgccgccg tagcgataat cgccgtcata
     3241 gctaacaaat aatgcagttt gctgaccagt cagtttgcgc tgactggtca atctcgtact
     3301 tataaatccg ccatcgccgc gcgacgattc gggaagaacg cgaggcgtcc cgggatcggt
     3361 tgaatgcctg cgcgagccat agtgcgcagt ggctggaatt ccacgttgca cacgcgcagt
     3421 tcacatcctt cgggcagacg cttcacaaaa cgctggaacg catcaagacc accagcatca
     3481 agtaccggaa cggcatccca cttcagaatc acaatccgtt tgccttcaag acgtgactcc
     3541 aggtccgtga acaagccttc agcagcagca aaaaacagcg ggccaataac gcgcagtacc
     3601 agaacatcgt ctggaacatc tacgactacc ggtgccaggc gagtcatacg tgcgatacga
     3661 cgcataaaca gcagcgatgc cagcacgatc cccacgctga tggcaataac catatcaaac
     3721 agcacggtca gcgacatgca cagcagcatg acaatgatgt catctttcgg tgcatgacgc
     3781 agcaagtcga ccactttatg cgcttcactc atgttccacg ccaccatcaa cagcagggct
     3841 gccatagcgg aaagcggcag ccaggagagc agcggtgcca gtaccagcag ggcaagaata
     3901 accagaatag agtggatcac cgccgagata ggggaagttg ccccggcacg gacgttagcg
     3961 gcagaacgcg cgatggcagc tgtagcggta ataccaccaa agaacggagc gataatattc
     4021 cccagtccct gtccaaccag ttcgctgttc gccttgtgtt tcgtcccggt cataccatcc
     4081 agtaccacgg cgcagagcag agattcgatt gcgccgagca ttgccattga gaatgccgca
     4141 ggcagcagtg tgcgaataga atcccaggtt agcgtgaatt ctgaattagg cagatcccac
     4201 ggcagcacca gttgcggcag cagttgcgga ataccgttac cctgagaacc atcggccagg
     4261 acgtagtgga attgcgaacc gatggtagca acatgtccgc cgagcaggtt aacaatcccc
     4321 atcaccgcgc aaccagccag caaggccgga aggtgaccgg gtaaacgaat gcccagacgc
     4381 ggccaaaaaa caagaatacc tagcgtcaca atgccaatgg cagcatcacc cacattaatg
     4441 gtcggcagcg ccataaataa tgcgccgact ttttgtagat aatgttccgg gacatgggcc
     4501 atttgcagac cgagaaaatc tttaatctgc atggtaccga tggtgatccc gatacccgag
     4561 gtgaaaccta aggtgacgga aaccggaata tactcaatca ggcgaccaaa gcgtgccaga
     4621 cccatcagaa tcaaaaagat ccccgacagc aaggtcgcaa ccagcagtcc tgccagtcca
     4681 aactgttgcg acacgggata gagaattacc acaaatgccg cagtcggacc ggaaacgcta
     4741 aagcgtgacc cacccgtcag agcaatgaca atccccgcaa cagctgcggt atataaaccg
     4801 tactggggtg ccacaccact accaatagcc aacgccatcg ccagcgggat agcaataatc
     4861 ccgacggtta tcccggcaat caggtcacgg gtaaaccgtg cggcagtata tttttctttc
     4921 cagcaagcgt cgatcagagc gcggaaaggc atcacatgtg aggaaaatat tttgttcaca
     4981 ataatgtttc atccgtgagc gcatcatctg tcaactaaat ggcaggtgaa ggaggcatag
     5041 gtcatacaaa tggatattac agacaaaaaa acccgccgca gcgggtcttt gagcgggttc
     5101 gattagtgtt cgaacatggc agagatcgat tcttcgttgc tgatacgacg aatcgcttcg
     5161 gccagcatac ctgacagggt cagagtacgc acgttcggca gtgatttgat ttcatcgctc
     5221 agcggaatgg tatcgcagac aacgacttca tcaattacag agttacgcag gttgttcgcc
     5281 gcgttgccag agaagatcgg gtgagtcgcg tacgcaaata cacgtttagc accacgttct
     5341 ttcagagctt cagcagcttt acacagcgta ccgccagtgt cgatcatatc atcgaccagt
     5401 acgcagtcac gacctgcaac gtcaccgatg atatgcatca cctgggaaac gttcgcacgc
     5461 ggacgacgtt tgtcgatgat tgccatatcg gtatcgttca gcagcttagc gatagcgcgg
     5521 gcacgcacaa cgccgccgat gtccggagaa accacaattg ggttatccag attcagctgc
     5581 agcatgtctt ccagcaggat cgggctacca aatacgttat caaccggaac gtcgaagaaa
     5641 ccctgaatct gttcagcgtg cagatccact gtcagcacac ggtcaacacc gacgctggag
     5701 aggaagtctg caaccacttt cgcagtgatt ggtacacgag cggaacggac gcgacggtcc
     5761 tggcgcgcat agccaaagta ggggataaca gcggtgatac gacctgcgga agcacgacgc
     5821 agggcatcaa ccataacgac taattccatc aggttgtcgt tagtaggggc acaagtggac
     5881 tggatgatga aaatatcacc accgcgtaca ttttcgttga tttgtacact gacttcgccg
     5941 tcgctaaagc gacctacagc ggcgtcgccg agtgaagtgt acaggcggtt ggcaatacgt
     6001 tgtgctagtt ccggggtggc gttaccagca aaaagcttca tatcaggcac gagaagaacc
     6061 tcaggcatgc gtccattggt ggaaagaatc tgccgaaaac tgtgcgggcc aggcatgatc
     6121 ctttccaggc ggtgtattaa agagcgcgat gcaacgtctg gaacaaggtg acgttgtcac
     6181 cgaaactcag cttgcccggc ttaaagcatg gctctgtgca atggggaaag attaacacct
     6241 ttcgccacaa agccattgag ccattccggg gcttgctcta gcacctggcg ggcttcagac
     6301 tctgtatcaa attcagcaaa gacacaggcc cctgtcccag tcaggcgcga cggggcgtat
     6361 tctaacagcc aggaaagcac cgcatcaacc tcgcgaaaac gttttcttgc gataacctcg
     6421 caatcattgc tgaattcaca ttttagcaac gtttctattg acctttttgg cgtattgcgc
     6481 gggagttcag gatctttaaa aatcactggt gtcggaatac ttacaccagg gtgcgccacc
     6541 agataccact tctctggcgg atccaccggc gttagtattt caccaacgcc ttcggcaaac
     6601 gcggcatgcc cccgaacaaa gacaggaaca tctgcgccca gcgtcagccc catttccgcc
     6661 agctcatcca tgcttagccc gcattgccag agatgattta atgccaccag gaccgtcgcg
     6721 gcattggatg aaccaccgcc gagaccgccg cccatcggca aacgcttgtc aatgctgata
     6781 ttcgcaccgc ttcccgtcgg aagacgcccg ctgtctgccg cagttttcat caacaaccgc
     6841 gctgcgcgaa cgatcaggtt atcttcatgt tccacgcctt caacgggcgt taacagacga
     6901 atatccccat cgtcacgaag ctcaatgctg atggtgtcgc cgtaatcaag aaactgaaac
     6961 agcgtttgca gcgtgtggta accatccgca cgctgaccgg taatgtataa aaacagatta
     7021 agttttgccg gagagggcca ctgtgtccgc attatttcac tatccagtta tccattttta
     7081 acttgatgcg ttgaccaccg tcggtgagtt ccatattggc tggcatcgca ggttgcgttt
     7141 tggtgtcata accaccataa acaaccttcc agtttttgcc attctggctg taggtaattt
     7201 cgctcaggcg gtactggtcg tccagtttgt agtcggttgc atcacccggt aaacctaaaa
     7261 tccattggcg caagctgttg agcggaattg gcattccggt caatttgcca atcatctctt
     7321 cggcgtcatc ggcggtataa cgctgacctt tattgtcgac taactgcacg ttacccggtt
     7381 gagcattcag ctccagttcc gtgctgccca atgggttagt gagcagcaga cggtagcgat
     7441 cctggccggt ttgctgccag aaaaagcggg cgtacacttt ttgttggtca gaaatataag
     7501 cgaacgcgcc gcgagtctga tactgattaa gattgcgcac gtcttgctga tgctgacgcc
     7561 attgtggcga atccgggctt ttgccaggac ctttgggcgt ggtaacggaa caggcagtga
     7621 gcacaagagc agccagcggt agcaggcgga taagacgaaa atcgggctgg ggcatagtga
     7681 tgacaagtcc ttgagatacg ttgcagttat aacccttaat gctagcgtta ccgtccgcta
     7741 tcgtctatgt tcaagttgtc ttaattgcca gaatctaacg gctttcggca attactccaa
     7801 aagggggcgc tctcttttat tgatcttacg catcctgtat gatgcaagca gactaaccat
     7861 atcaacgttg gtattatttc ccgcagacat gaccctttta gcactcggta tcaaccataa
     7921 aacggcacct gtatcgctgc gagaacgtgt atcgttttcg ccggataagc tcgatcaggc
     7981 gcttgacagc ctgcttgcgc agccgatggt gcagggcggc gtggtgctgt cgacgtgcaa
     8041 ccgcacggaa ctttatctta gcgttgaaga gcaggataac ctgcaagagg cgttaatccg
     8101 ctggctttgc gattatcaca atcttaatga agaagatctg cgtaaaagcc tctactggca
     8161 tcaggataac gacgcggtta gccatttaat gcgtgttgcc agcggcctgg attcactggt
     8221 tcttggggag ccgcagatcc tcggtcaggt taaaaaagcg tttgccgatt cgcaaaaagg
     8281 tcatatgaag gccagcgaac tggaacgcat gttccagaaa tctttctctg tagcgaaacg
     8341 cgttcgcact gaaacagata tcggtgccag tgctgtgtct gtcgcttttg cggcttgtac
     8401 gctggcgcgg cagatctttg aatcgctctc tacggtcaca gtgttgctgg taggcgcggg
     8461 cgaaaccatc gagctggtag cgcgtcatct gcgcgaacat aaagtacaga agatgattat
     8521 cgccaaccgc actcgcgaac gtgcccaaat tctggcagat gaagttggcg cggaagtgat
     8581 tgccctgagt gagatcgacg aacgtctgcg cgaagccgat atcatcatca gttccaccgc
     8641 cagcccgtta ccgattatcg ggaaaggcat ggtggagcgc gcattaaaaa gtcgtcgtaa
     8701 ccaaccaatg ctgttggtgg atattgccgt tccgcgcgat gttgagccgg aagttggcaa
     8761 actggcgaat gcttatcttt atagtgtgga cgatctgcaa agcatcattt cgcacaacct
     8821 ggcgcagcgt aaagccgcag cggttgaggc ggaaactatt gtcgctcagg aaaccagcga
     8881 atttatggcg tggctgcgag cacaaagcgc cagcgaaacc attcgcgagt atcgcagcca
     8941 ggcagagcac gttcgcgatg agttaaccgc caaagcgtta gcggcccttg agcagggcgg
     9001 cgacgcgcaa gccattatgc aggatctggc atggaaactg actaaccgct tgatccatgc
     9061 gccaacgaaa tcacttcaac aggccgcccg tgacggggat aacgaacgcc tgaatattct
     9121 gcgcgacagc ctcgggctgg agtagcagta catcattttc tttttttaca gggtgcattt
     9181 acgcctatga agccttctat cgttgccaaa ctggaagccc tgcatgaacg ccatgaagaa
     9241 gttcaggcgt tgctgggtga cgcgcaaact atcgccgacc aggaacgttt tcgcgcatta
     9301 tcacgcgaat atgcgcagtt aagtgatgtt tcgcgctgtt ttaccgactg gcaacaggtt
     9361 caggaagata tcgaaaccgc acagatgatg ctcgatgatc ctgaaatgcg tgagatggcg
     9421 caggatgaac tgcgcgaagc taaagaaaaa agcgagcaac tggaacagca attacaggtt
     9481 ctgttactgc caaaagatcc tgatgacgaa cgtaacgcct tcctcgaagt ccgagccgga
     9541 accggcggcg acgaagcggc gctgttcgcg ggcgatctgt tccgtatgta cagccgttat
     9601 gccgaagccc gccgctggcg ggtagaaatc atgagcgcca gcgagggtga acatggtggt
     9661 tataaagaga tcatcgccaa aattagcggt gatggtgtgt atggtcgtct gaaatttgaa
     9721 tccggcggtc atcgcgtgca gcgtgttcct gctacggaat cgcagggtcg tattcatact
     9781 tctgcttgta ccgttgcggt aatgccagaa ctgcctgacg cagaactgcc ggacatcaac
     9841 ccagcagatt tacgcattga tactttccgc tcgtcagggg cgggtggtca gcacgttaac
     9901 accaccgatt cggcaattcg tattactcac ttgccgaccg ggattgttgt tgaatgtcag
     9961 gacgaacgtt cacaacataa aaacaaagct aaagcacttt ctgtacttgg tgctcgtatc
    10021 cacgctgcag aaatggcaaa acggcaacag gccgaagcgt ctacccgtcg taacctgctg
    10081 gggagtggcg atcgcagcga ccgtaaccgt acttacaact tcccgcaggg gcgcgttacc
    10141 gatcaccgca tcaacctgac gctctaccgt ctggatgaag tgatggaagg taagctggat
    10201 atgctgattg aaccgattat ccaggaacat caggccgacc aactggcggc gttgtccgag
    10261 caggaataat ggaatatcaa tactggttac gtgaagcaat aagccaactt caggcgagcg
    10321 aaagcccacg ccgtgacgct gaaatcttat tggagtatgt cactggcagg gggcggactt
    10381 ttattctggc ctttggtgaa acgcagctga ctgacgaaca atgtcagcaa cttgatgcgc
    10441 tactgacacg tcgtcgcgat ggtgaaccca ttgctcattt aaccggggtg cgagaattct
    10501 ggtcgttgcc gttatttgtt tcgccagcga ccttaattcc gcgcccggat acggagtgtc
    10561 tggtggagca ggcactggcg cggttgcctg aacaaccttg ccgtattctc gatctcggga
    10621 cgggtaccgg ggcgattgcg cttgcgctgg ctagcgagcg cccggactgc gaaattatcg
    10681 ctgtagatcg tatgcctgat gctgtctccc tggcacaacg taatgcccag catctggcga
    10741 tcaaaaatat ccacattctg caaagcgact ggtttagcgc gctagccggg cagcagtttg
    10801 cgatgattgt cagcaatccg ccgtatattg acgagcagga cccacatctt caacaaggcg
    10861 atgtccgctt tgagccgctc actgcgctgg ttgcggcaga cagtggaatg gcagacatcg
    10921 tgcatatcat cgaacagtcg cgtaacgcgc tggtatccgg cggctttctg cttctggaac
    10981 atggctggca gcagggcgaa gcggtgcgac aagcatttat cctcgcgggg tatcatgacg
    11041 tcgaaacctg ccgtgactat ggtgataacg agcgcgtaac gctcggccgc tattatcaat
    11101 gacaagtttt tctacactgc ttagtgttca tcttattagt atcgcgcttt ctgttgggct
    11161 attaacctta cgtttctggc tacgttatca gaagcatcca caggcatttg ctcgctggac
    11221 gcgcattgtg ccgccggttg tcgatacggt gttattgtta agcggcattg cgttgatggc
    11281 taaagcgcac atcctgccat tttccgggca ggcacagtgg ctgactgaaa agctgtttgg
    11341 agttatcatt tatatcgttt tgggttttat tgcactcgat tatcgtcgta tgcacagtca
    11401 gcaggcgcgc attattgcct tcccgctggc gttggtggtg ctgtacatca tcattaaact
    11461 cgccaccaca aaagtaccgt tactggggta agtcatgaga tcgttagctg atttcgaatt
    11521 taataaagcg ccattgtgcg aaggcatgat cctggcttgc gaagcaatcc gccgcgattt
    11581 tccctcgcaa gatgtttacg acgaactgga gcgtctcgtt agtctggcga aggaagaaat
    11641 cagccagctt ctgcctttag aagagcagct ggaaaaactg atcgcgctgt tttacggcga
    11701 ctggggattt aaagcctcac gcggcgttta tcgtctttcc gatgcattat ggctggacca
    11761 ggtgttaaag aatcgacagg cagtgcggta tcattaggtg cggttttatt atgggtcgcg
    11821 aatcgtctcg atttgccgct gctgccggtg attttcccta cgcagctgat attgcgcatt
    11881 gaatgtccgg atggcgaaat ttggctgatt aatcctttta acggtgaatc gttaagcgaa
    11941 catatgctgg acgtatggtt aaagggaaat atcagcccgt cggcggaact gttttatgaa
    12001 gaccttgatg aagctgataa cattgaggta atccgcaaat tgttggatac actcaaagcc
    12061 tcgttgatgg aagaaaatca gatggagctg gcgttacgca ccagcgaagc tttattacaa
    12121 ttcaacccgg aagatcccta tgaaattcgc gatcgcgggt tgatttatgc gcaactggat
    12181 tgcgaacacg ttgcgttgaa cgatttaagt tatttcgttg aacagtgtcc ggaagacccg
    12241 atcagcgaaa tgatccgtgc gcaaataaat aacatcgcgc ataaacatat tgtgctgcat
    12301 taattaatcg acattttact caagattaag gcgatcctat gaaacaaaaa gtggttagca
    12361 ttggcgacat caacgtagca aatgacctgc cgttcgtact gtttggtggt atgaacgtgc
    12421 tggaatctcg cgatctggcg atgcgcattt gcgagcacta cgtaaccgtg acccagaaac
    12481 tgggtatccc ttacgtgttc aaagcctctt ttgacaaagc caaccgctcc tccatccact
    12541 cttatcgtgg accgggcctg gaagaaggga tgaaaatctt ccaggagttg aagcagactt
    12601 ttggcgtgaa aattatcacc gacgttcacg aaccaagtca ggcacagccc gttgctgatg
    12661 tcgtggatgt gattcagttg ccggcgtttc ttgctcgcca gactgacctg gttgaagcca
    12721 tggcgaaaac cggtgcggta attaacgtca agaaaccaca gtttgtcagc ccgggacaga
    12781 tgggtaatat cgttgataaa ttcaaagaag gcggcaacga aaaagtgatt ctttgcgatc
    12841 gcggtgctaa cttcggctat gacaacctgg ttgtcgatat gctgggcttc agcattatga
    12901 agaaagtgtc tggtaactcg ccggtgattt tcgacgtgac ccacgcactg caatgccgcg
    12961 atccgtttgg cgcagcttcc ggtggtcgtc gtgctcaggt ggctgagctg gcacgagccg
    13021 gtatggcggt aggtctggcg gggctgttta ttgaagcgca tccggatccg gaacatgcga
    13081 aatgtgatgg tccatccgcg ctgccgctgg ctaaactgga accgttcctc aagcagatga
    13141 aagcgattga tgatctggtg aaaggtttcg aagaactgga taccagcaag taatcttttt
    13201 tgcttgaaaa ataaagcctt agcgttctgc gttaagactt ttttcatggg tgccagatac
    13261 aaaaa
//
LOCUS       DAJVQG010000112        12997 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJVQG010000112 DAJVQG010000000
VERSION     DAJVQG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12997
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.112"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            96..1391
                     /locus_tag="OQM83_002918"
     CDS             96..1391
                     /locus_tag="OQM83_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCS1521126.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(1437..2030)
                     /gene="yhgN"
                     /locus_tag="OQM83_002919"
     CDS             complement(1437..2030)
                     /gene="yhgN"
                     /locus_tag="OQM83_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCS1521127.1"
                     /translation="MNAIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2222..3325
                     /gene="asd"
                     /locus_tag="OQM83_002920"
     CDS             2222..3325
                     /gene="asd"
                     /locus_tag="OQM83_002920"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCS1521128.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3598..5784
                     /gene="glgB"
                     /locus_tag="OQM83_002921"
     CDS             3598..5784
                     /gene="glgB"
                     /locus_tag="OQM83_002921"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCS1521129.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5781..7754
                     /gene="glgX"
                     /locus_tag="OQM83_002922"
     CDS             5781..7754
                     /gene="glgX"
                     /locus_tag="OQM83_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCS1521130.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7772..9067
                     /gene="glgC"
                     /locus_tag="OQM83_002923"
     CDS             7772..9067
                     /gene="glgC"
                     /locus_tag="OQM83_002923"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCS1521131.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9067..10500
                     /gene="glgA"
                     /locus_tag="OQM83_002924"
     CDS             9067..10500
                     /gene="glgA"
                     /locus_tag="OQM83_002924"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCS1521132.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10519..12966
                     /gene="glgP"
                     /locus_tag="OQM83_002925"
     CDS             10519..12966
                     /gene="glgP"
                     /locus_tag="OQM83_002925"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCS1521133.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEMR
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3040 a         3228 c         3577 g         3152 t
ORIGIN      
        1 ttccaccagt gtcaaaatct cgacggggcg tttgttatta cccctccttt gatgcctggt
       61 ccggcgttgc tggttgacga tatcgtggat tctgcatgga cgctgacagt tctgacagca
      121 ctgttacgcc aggcaggttg cccgacggtt tatcctcttg cccttgcttc tacctcggta
      181 aaaaattgat gaatctttca gccaatgcac aagcaactct cctgctaacc agcgattttt
      241 ctcgcgcggc ggcgagtgag tataaacctc ttagtaatag tgaatggggg aagtttgcat
      301 tatggctgaa gcaccaacgt atcagtcccg ccgagcttct ggtgccgcaa ccgcaagaga
      361 aacttacagg ctggagcgat ccgcgtattt ctcaggagcg tattcttggc ttgctggcgc
      421 gtggtcatag tctggcgttg gcggtagata agtggcaacg cgccggttta tggatcttaa
      481 cccgcggaga tgctgattat cccgttcgct tgaaaaaccg attgcgaacg gatgcacctc
      541 ccgttttatt tggctgcggg aataaagcat tactgcaagc ggaaggtatg gcgattgttg
      601 gctcgcgaga tgctccgact gacgatttgc gctataccca acaactggcc gcgaaactgg
      661 cccaacaggg gatttgcgtt atctctggtg gtgcgcgagg tattgatgaa tgtgcaatgg
      721 cgtcggcact ggaggccggg ggaactgccg ttggcgtatt agctgatagc ttgttaaaaa
      781 cgagtacgtt agtgaaatgg cgtgaagggc ttatagcagg caacctggtg ttgatttcgc
      841 cgttttaccc agaggtacgt ttcaccgtcg gcaatgcgat ggcgcgaaat aaatatattt
      901 attgccttgc tgaaagcgca atggttgtac gtgcgggaat gaccggtgga acgataaccg
      961 gggcgatgga ggcattaaaa catcagtggc tgcctgtgca ggttaaacca aatcaggata
     1021 tgcaatcagc caattcacga ttagtagaaa atggggcgtc atggagtgct gaacaggctg
     1081 agaatgtgac gatcagactg ccagacgttc ctgggctgat gtatgacaga gcactccgta
     1141 acgctcaacc agaactgttt tcgctgcatg aagatgacgc aaattacgca gtaatgcccg
     1201 cgtatacgcc tgtcgatttt tatcaactct ttgtggcgga actggcgatc cttgcaaagg
     1261 aatcgataag tattgaaagg ctggcgtctt gtactggttt aaccatcgaa caaattagtg
     1321 tgtggctgaa ccgcgcagaa gaagagggaa gggttatccg attgggcgaa ggtcattatc
     1381 agttcaggta ataaaacagg tgccgcgtct ggttgtgtgc agcacctgtt tgaaccttac
     1441 cccttcatcc acattcgaat gccgtcgagg aacatctggg ttgccatcat caccagaatc
     1501 aatcccatca ggcgttcaag tgcgttcacc cctttctcgc ccagcagacg taaaaatagc
     1561 gaagactgta gcaggatgac aaaggtgccg ccccaggcca gcagcagagc aatcaccaga
     1621 tgtcccatct gattcgggta ctgatgagac aacaacatca gcgtggcgag aatagtcggc
     1681 ccggcgacta acggaattgc caacggcacg ataaatggct cttcacctgc cggaagcccg
     1741 ctgctatttc ctgaagcgct ggggaaaatc atcttaatgg cgattagaaa cagaatgatg
     1801 ccgccagaaa tggagacggt ttctgctcgc aggctgagaa atgccagaat tttctcaccc
     1861 gcaaacagga acaccagcat caccaggaga gcaataagca actctcgcac catgattgcc
     1921 cgccgtcttt tcggttcagt atgtttcagt acggacatga aaataggcag gtttccgagc
     1981 ggatccataa tcaggatcaa taaaactgct gcagaaatga ttgcattcat aactcaaatt
     2041 ccctgataat tgccgcggac tttctgcgtg ctaacaaagc aggataagtc gcattactga
     2101 tggcttcgct atcattgatt aatttcactt gcgactttgg ctgctttttg tatggtgaaa
     2161 gatgtgccaa gaggagaccg gcacatttat acagcacaca tctttgcagg aaaaaacgct
     2221 tatgaaaaat gttggtttta tcggctggcg cggtatggtc ggctccgttc tcatgcaacg
     2281 catggttgaa gagcgcgact tcgacgccat tcgccctgtc ttcttttcta cttctcagct
     2341 tggccaggct gcgccgtctt ttggcggaac cactggcaca cttcaggatg cctttgatct
     2401 ggaggcgcta aaggccctcg atatcattgt gacctgtcag ggcggcgatt ataccaacga
     2461 aatctatcca aagcttcgtg aaagcggatg gcaaggttac tggattgacg cagcatcgtc
     2521 tctgcgcatg aaagatgacg ccatcatcat tcttgacccc gtcaatcagg acgtcattac
     2581 cgacggatta aataatggca tcaggacttt tgttggcggt aactgtaccg taagcctgat
     2641 gttgatgtcg ttgggtggtt tattcgccaa tgatcttgtt gattgggtgt ccgttgcaac
     2701 ctaccaggcc gcttccggcg gtggtgcgcg acatatgcgt gagttattaa cccagatggg
     2761 ccatctgtat ggccatgtgg cagatgaact cgcgaccccg tcctctgcta ttctcgatat
     2821 cgaacgcaaa gtcacaacct taacccgtag cggtgagctg ccggtggata actttggcgt
     2881 gccgctggcg ggtagcctga ttccgtggat cgacaaacag ctcgataacg gtcagagccg
     2941 cgaagagtgg aaagggcagg cggaaaccaa caagatcctc aacacatctt ccgtaattcc
     3001 ggtagatggt ttatgtgtgc gtgtcggggc attgcgctgc cacagccagg cattcactat
     3061 taaattgaaa aaagatgtgt ctattccgac cgtcgaagaa ctgctggctg cgcacaatcc
     3121 gtgggcgaaa gtcgttccga acgatcggga aatcactatg cgtgagctaa ccccagctgc
     3181 cgttaccggc acgctgacca cgccggtagg ccgcctgcgt aagctgaata tgggaccaga
     3241 gttcctgtca gcctttaccg tgggcgacca gctgctgtgg ggggccgcgg agccgctgcg
     3301 tcggatgctt cgtcaactgg cgtaatcttt attcattaaa tctggggcgc gatgccgccc
     3361 ctgttagtgc gtaatacagg agtaagcgca gatgtttcat gatttaccgg gagttaaata
     3421 gagcattggc tattctttaa gggtggctga atacatgagt attcacagcc ttacctgaag
     3481 tgaggacgac gcagagagga tgcacagagt gctgcgccgt tcaggtcaaa aaaatgtcac
     3541 aaccagaagt caaaaatcca attggatggg gtgacacaat aaaacaggaa gacaagcatg
     3601 tccgatcgta tcgatagaga cgtgattaac gcgctaattg caggccattt tgcggatcct
     3661 ttttccgtac tgggaatgca taaaaccacc gcgggactgg aagtccgtgc ccttttaccc
     3721 gacgctaccg atgtgtgggt gattgaaccg aaaaccgggc gcaaactcgc aaaactggag
     3781 tgtctcgact cacggggatt ctttagcggc gtcattccgc gacgtaagaa ttttttccgc
     3841 tatcagttgg ctgttgtctg gcatggtcag caaaacctga ttgatgatcc ttaccgtttt
     3901 ggtccgctaa tccaggaaat ggatgcctgg ctattatctg aaggtactca cctgcgcccg
     3961 tatgaaacct taggcgcgca tgcagatact atggatggcg tcacaggtac gcgtttttct
     4021 gtctgggctc caaacgcccg tcgggtctcg gtggttgggc aattcaacta ctgggacggt
     4081 cgccgtcacc cgatgcgcct gcgtaaagag agcggcattt gggaactgtt tatccctggg
     4141 gcgcataacg gtcagctcta taaatacgag atgattgatg ccaatggcaa cttgcgtctg
     4201 aagtccgacc cttatgcctt cgaagcgcaa atgcgcccgg aaaccgcgtc tcttatttgc
     4261 gggctgccgg aaaaggttgt acagactgaa gagcgcaaaa aagcgaatca gtttgatgcg
     4321 ccaatctcta tttatgaagt tcacctgggc tcctggcgtc gccacaccga taacaatttc
     4381 tggttgagct accgcgagct ggccgatcaa ctggtgcctt atgctaaatg gatgggcttt
     4441 acccacctcg aactactgcc cattaacgag catcccttcg atggcagttg gggttatcag
     4501 ccaaccggcc tgtatgcacc aacccgccgt tttggtactc gcgacgactt ccgttatttc
     4561 attgatgccg cacacgcagc gggtctgaac gtgattctcg actgggtgcc aggccacttc
     4621 ccgaccgatg actttgcgct tgccgaattt gatggcacga acttgtatga acacagcgat
     4681 ccgcgcgaag gctatcatca ggactggaat acgctgatct acaactatgg tcgccgtgaa
     4741 gtcagtaact tcctcgtcgg taacgcgctt tactggattg aacgttttgg tattgatgcg
     4801 ctgcgcgtcg atgcggtggc gtcaatgatt tatcgcgact acagccgtaa agagggggag
     4861 tggatcccga acgaatttgg cgggcgcgag aatcttgaag cgattgaatt cttgcgtaat
     4921 accaaccgta ttcttggtga gcaggtttcc ggtgcggtga caatggctga ggagtctacc
     4981 gatttccctg gcgtttctcg tccgcaggat atgggcggtc tgggcttctg gtacaagtgg
     5041 aacctcggct ggatgcatga caccctggac tacatgaagc tcgacccggt ttatcgtcag
     5101 tatcatcacg ataaactcac cttcgggatt ctctacaact acactgaaaa cttcgtcctg
     5161 ccgttgtcgc atgatgaagt ggtccacggt aaaaaatcga ttctcgaccg catgccgggc
     5221 gacgcatggc agaaattcgc taacctgcgc gcctactatg gctggatgtg ggcattcccg
     5281 ggcaagaaac tgctgttcat ggggaacgaa tttgcccaag gccgtgagtg gaaccatgac
     5341 gccagcctcg actggcatct gttggaaggc ggcgataact ggcaccacgg tgtccagcgt
     5401 ctggtgcgcg atctgaacct cacctaccgc caccataaag caatgcatga actggatttt
     5461 gacccgtacg gctttgaatg gctagtggtg gatgacaaag aacgctcggt gctgatcttt
     5521 gtgcgtcgcg ataaagaggg taacgaaatt atcgttgcca gtaactttac gccggtgccg
     5581 cgtcatgatt atcgcttcgg cattaatcag ccaggcaaat ggcgtgaaat cctcaatacc
     5641 gattccatgc actatcacgg cagtaatgca ggcaatggcg gcacggtaca cagcgatgag
     5701 attgccagcc acggtcgtca gcattcacta agcctgacac taccaccgct ggccactatc
     5761 tggctggttc gggaggcaga atgacacgac tcgccattgg caaacccgct cccctcggcg
     5821 cgcattacga cggtcagggc gtcaacttca cacttttctc cgctcatgcc gagcgggtag
     5881 agctgtgtgt ctttgacgcc aatggccagg aacatcgcta tgacttgcca gggcacagtg
     5941 gcgacatttg gcacggttat ctgccggatg cgcgcccggg tttgcgttat ggttatcgcg
     6001 ttcatggccc ctggcaaccc gccgaggggc atcgctttaa cccggcgaag ttgttgattg
     6061 atccttgcgc gcggcaaatt gaaggggaat tcaaagataa cccgctgctg cacgccggtc
     6121 ataatgaacc tgactatcgc gacaacgccg ccattgcgcc gaaatgcgta gtggtggttg
     6181 atcactatga ctgggaagat gatgccccgc cgcgcacgcc gtggggcagc accatcattt
     6241 atgaagccca tgttaaagga ttaacgtacc tgcacccgga gatcccggtc gagatccgtg
     6301 gcacttataa agccctcggg catccggtga tgatcaacta tttgaaacag ttgggcatta
     6361 ccgcgctgga actgctgccg gtggcgcagt ttgccagtga accacgtctg caacgcatgg
     6421 ggctaagtaa ctactggggt tacaacccgg tggcgatgtt tgcgctgcat ccggcgtatg
     6481 cctgctcgcc agaaacggcg ctgcatgagt ttcgcgatgc aatcaaagca ctgcataaag
     6541 cgggtatcga agtcattctt gatatcgtgc tcaaccatag tgcggaactg gacctcgacg
     6601 gcccgttatt ctcgctgcgt gggatcgaca accgtagcta ttattggata agagaagacg
     6661 gcgattatca caactggacc ggttgtggca acacgctcaa tttgagtcat ccggcggtgg
     6721 tggattatgc ctgcgcctgc ctgcgttatt gggtagaaac ctgtcacgtc gatggtttcc
     6781 gctttgatct ggcggcagtc atgggccgta cgccagagtt ccgtcaggat gcgccgttgt
     6841 ttaccgctat ccagaactgc ccggtgctct cgcaggtgaa gttaattgct gaaccgtggg
     6901 atatcgccct tggtggttat caggtgggaa atttcccgcc gctgtttgcc gagtggaacg
     6961 atcatttccg cgatgctgcc cgtcgtttct ggctacatta tgatttgcct ctgggggcgt
     7021 ttgccgggcg ttttgctgcc tccagcgatg tttttaaacg taatggtcgt ctgccgagtg
     7081 ccgcgattaa tctcgtcact gcgcatgacg gttttacgct tcgcgactgc gtttgcttca
     7141 accataaaca caatgaagca aacggagaag aaaatcgcga cgggaccaac aacaattaca
     7201 gtaacaatca tggtaaagaa gggttaggcg gttctcttga cctggttgaa cggcggcgcg
     7261 acagtattca cgccctgtta acaacgttgt tgctctccca gggtacgccg atgttactgg
     7321 ccggtgacga acacggtcac agccagcatg gcaataacaa tgcctactgt caggataacc
     7381 aattaacctg gttggactgg tcgcaggcaa gcagtggttt aaccgcattt accgccgcgt
     7441 taatccatct gcgcaagcgc attcccgctt tggtggagaa tcgctggtgg gaagaaggcg
     7501 acggcaatgt ccgttggcta aatcgatatg ctcaaccttt aagcacggat gagtggcaaa
     7561 acgggccgaa acagctgcaa attctgctct cggatcgctt tttgatcgca attaacgcca
     7621 cgcttgaggt aacagagatt gttttacctg ctggggagtg gcacgccatt cccccattcg
     7681 ctggagagga taacccagtg attacggctg tctggcaggg acctgcacac ggattgtgtg
     7741 tgttccagag atgataaaaa aggagttagt catggttagt ttagagaaga acgatcactt
     7801 aatgttggcg cgccagctgc cattgaaatc tgttgccctg atactggcgg gaggacgtgg
     7861 tacccgcctg aaggatttaa ccaataagcg agcaaaaccg gccgtacact tcggcggtaa
     7921 gttccgcatt attgactttg cgctgtctaa ctgcatcaac tctgggatcc gtcgtatggg
     7981 cgtgatcacc cagtaccagt cccacactct ggtgcagcac attcagcgcg gctggtcatt
     8041 cttcaatgaa gaaatgaacg agtttgtcga tctgctgcca gcacagcaga gaatgaaagg
     8101 ggaaaactgg tatcgcggca ccgcagatgc ggtcacccaa aacctcgaca ttatccgccg
     8161 ttataaagcg gaatacgtgg tgatcctggc gggcgaccat atctacaagc aagactactc
     8221 gcgtatgctt atcgatcacg tcgaaaaagg cgcacgttgc accgttgctt gtatgccagt
     8281 accgattgaa gaagcctccg catttggcgt tatggcggtt gatgagaacg ataaaattat
     8341 cgaattcgtt gaaaaacctg ctaacccacc gtcaatgccg aacgatccga gcaaatctct
     8401 ggcgagtatg ggtatctacg tctttgacgc cgactatctg tatgaactgc tggaagaaga
     8461 cgatcgtgat gagaactcca gccacgactt tggcaaagat ttgattccca agatcaccga
     8521 agccggtctg gcctatgcgc acccgttccc gctctcttgc gtacaatccg acccggatgc
     8581 cgagccgtac tggcgcgatg tgggtacgct ggaagcttac tggaaagcga acctcgatct
     8641 ggcctctgtg gtgccggaac tggatatgta cgatcgcaat tggccaattc gcacctacaa
     8701 tgaatcatta ccgccagcga aattcgtgca ggatcgctcc ggtagccacg ggatgaccct
     8761 taactcactg gtatccggcg gctgtgtgat ctccggttcg gtggtggtgc agtccgttct
     8821 gttctcgcgc gttcgcgtga attcattctg caacattgat tccgccgtat tgttaccgga
     8881 agtatgggta ggtcgctcgt gccgtctgcg ccgctgcgtc atcgatcgtg cttgtgttat
     8941 tccggaaggc atggtgattg gtgaaaacgc agaggaagat gcacgtcgtt tctatcgttc
     9001 agaagaaggc atcgtgctgg taacgcgcga aatgctacgg aagttagggc ataaacagga
     9061 gcgataatgc aggttttaca tgtatgttca gagatgttcc cgctgcttaa aaccggcggt
     9121 ctggctgatg ttattggggc attacccgca gcacaaatcg cagacggcgt tgacgctcgc
     9181 gtactgttgc ctgcatttcc cgatattcgc cgtggcgtga ccgatgcgca ggtagtatcc
     9241 cgtcgtgata ccttcgccgg acatatcacg ctgttgttcg gtcattacaa cggggttggc
     9301 atatacctga ttgacgcgcc gcatctctat gatcgtccgg gaagcccgta tcacgatacc
     9361 aacttatttg cctataccga caacgtattg cgttttgcgc ttctggggtg ggttggggca
     9421 gaaatggcca gcgggcttga cccattctgg cgtcctgatg tggtgcatgc gcacgactgg
     9481 catgcaggcc ttgcgcctgc gtatctggcg gcgcgcgggc gtcctgcgaa gtcggtgttt
     9541 actgtgcaca acctggccta tcaaggcatg ttttatgcac atcacatgaa tgacatccaa
     9601 ttgccatggt cattctttaa tattcatggg ctggaattca acggacaaat ctctttcctg
     9661 aaggccggtc tgtactatgc cgatcacatt acggcggtca gtccaaccta cgctcgcgag
     9721 atcaccgaac cgcagtttgc ctacggtatg gaaggtctgt tgcaacagcg tcatcgtgaa
     9781 gggcgtcttt ccggcgtact gaacggcgtg gacgagaaaa tctggagtcc agagacggac
     9841 ttactgttgg cctcgcgcta cacccgcgat acgttggaag ataaagcgga aaataagcgc
     9901 cagttacaaa tcgcaatggg gcttaaggtt gacgataaag tgccgctttt tgcagtggtg
     9961 agccgtctga ccagccagaa aggtctcgac ctggtgctgg aagccttacc gggtcttctg
    10021 gagcagggcg ggcagctggc gctactcggc gcgggcgatc cggtgctgca ggaaggtttc
    10081 cttgcggcgg cagcggaata ccccggtcag gtgggcgttc agattggcta tcacgaagca
    10141 ttttcgcatc gcattatggg cggcgcggac gtcattctgg tgcccagccg ttttgaaccg
    10201 tgcggcttaa cgcaacttta tggattgaag tacggtacgc tgccgttagt gcggcgcacc
    10261 ggtgggcttg ctgatacggt ttctgactgt tctcttgaga accttgcaga tggcgtcgcc
    10321 agtgggtttg tctttgaaga taataatgcc tggtcgctgt tacgggctat tcggcgtgct
    10381 tttgtactgt ggtcccgtcc ttcactgtgg cggtttgtgc aacgtcaggc tatggcaatg
    10441 gattttagct ggcaggtcgc ggcgaagtcg taccgtgagc tttactatcg cttgaaatag
    10501 ttttcaggaa acgcctatat gaatgctccg tttacatatt catcgcccac gcttagcgta
    10561 gaagctctta agcactctat cgcttacaag ctgatgttta cgattggaaa ggacccggtc
    10621 gtcgccaata aacatgaatg gctgaacgca acgttatttg ctgtgcgcga tcgtctcgtg
    10681 gagcgctggt tacgttcaaa ccgtgcccag ttgtcgcaag aaacacgtca ggtttactac
    10741 ctgtcgatgg agtttttgat tggccgtacg ctctccaacg ccatgttgtc gctaggaatt
    10801 tacgaagatg tacagggcgc actggaagcg atggggttaa atctcgaaga gctgattgat
    10861 gaagaaaatg acccaggcct cggtaacggt ggcctgggac gtctggcggc ttgcttcctt
    10921 gattctctgg cgacgttagg gttgccgggg cgcggttacg gcatccgcta tgactacggt
    10981 atgttcaagc agaacatcgt taacggtagc cagaaagagt cgccagacta ctggctggaa
    11041 tacggtaacc cgtgggaatt caaacgccac aacacgcgct ataaagtccg ttttggcggt
    11101 cgcattcagc aggaaggtaa aaaaacgcgc tggattgaaa ccgaagagat tctgggagtc
    11161 gcttacgatc agataatccc tggttacgac accgacgcga ccaacacgct gcgtttgtgg
    11221 agtgcgcaag ccagtagcga aattaacctc ggtaaattca accagggtga ctacttcgcg
    11281 gcagtggaag ataaaaacca ctccgagaac gtatctcgcg tactgtatcc ggatgactcc
    11341 acctactccg ggcgtgagct gcgcctgcgt caggaatact tcctggtttc ctcgaccatt
    11401 caggacattt taagccgcca ttatcagttg cataaaacct acgataacct ggcggataaa
    11461 atcgcgattc atctcaatga tacccatccg gtactgtcga ttcctgagat gatgcgtctg
    11521 ctgatcgatg agcaccaatt tagctgggac gacgcgtttg aggtgtgttg tcaggtcttc
    11581 tcctacacta accacacgct gatgagcgag gcgctggaaa cctggccggt tgatatgctg
    11641 ggtaaaattc tgccgcgtca cctgcagatc atctttgaaa tcaacgacta tttcctgaaa
    11701 accttgcagg aacagtatcc gaacgatacc gatctgctgg gacgggcgtc gatcattgat
    11761 gaatccaacg gtcgtcgtgt gcgtatggcc tggctggcgg ttgttgtgag ccacaaagtt
    11821 aacggtgtat cggaactgca ctctaatctg atggtgcaat cgttgtttgc cgactttgcg
    11881 aaaatcttcc cgggtcgttt caccaacgtc accaacggtg tgacgccgcg tcgctggctg
    11941 gcggtagcga acccatcgct ttcagccgtg ctggacgaac acctgggccg taactggcgc
    12001 accgacctta gcctgcttaa tgagctgcaa caacactgtg atttcccaat ggttaatcac
    12061 gctgtgcatc aggcgaagct ggagaacaaa aagcgtctgg cagagtatat cgcccagcag
    12121 ctgaatgtgg tggtgaatcc aaaggcgttg ttcgatgtac aaatcaaacg tattcacgaa
    12181 tacaaacgtc aattgatgaa tgtgttgcat gtgattaccc gctataaccg catcaaggcc
    12241 gacccggatg cgaagtgggt accgcgcgtg aatatttttg gcggtaaggc ggcttcggcc
    12301 tattacatgg cgaagcacat tattcatttg atcaatgacg tagcgaaagt gatcaacaac
    12361 gatccgcaga ttggcgataa gctgaaagtc gtgttcatcc cgaactacag cgttagcctg
    12421 gcgcagttga tcattccggc ggcagatctg tctgaacaga tttcgctggc agggacggaa
    12481 gcttccggca ccagtaacat gaagtttgcg cttaacggtg cgctgactat cggtacgttg
    12541 gacggtgcga atgtcgagat gcgggatcat gtcggtgctg acaatatctt tatttttggt
    12601 aacacagcgg aagaagtgga agaactgcgt cgtcagggct acaaaccgcg tgaatactac
    12661 gagaaagatg aggagctgca tcaggtgctg acgcaaatcg gcagcggtgt attcagtccg
    12721 gaagatccgg gtcgctatcg cgatctggtt gattcgctga tcaacttcgg cgatcactat
    12781 caggttctgg cggattatcg cagctatgtc gattgtcagg ataaagtcga cgaactctac
    12841 gagcttcagg aagagtggac cgcaaaagcg atgctgaaca ttgccaatat gggctacttc
    12901 tcttctgacc gtactatcaa agagtacgcc gatcatatct ggcatatcga tccggtgaga
    12961 ttgtaagttc accaataaat agaacggggc caaaggg
//
LOCUS       DAJVQG010000113        12960 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJVQG010000113 DAJVQG010000000
VERSION     DAJVQG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12960
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.113"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..481
                     /gene="umuC"
                     /locus_tag="OQM83_002926"
     CDS             <1..481
                     /gene="umuC"
                     /locus_tag="OQM83_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCS1521134.1"
                     /translation="RQAICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNS
                     ASVKLLTPTQDSRDIINAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNA
                     PRPGSEQLMTVMDTLNAKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(527..1057)
                     /gene="dsbB"
                     /locus_tag="OQM83_002927"
     CDS             complement(527..1057)
                     /gene="dsbB"
                     /locus_tag="OQM83_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCS1521135.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1203..2744)
                     /gene="nhaB"
                     /locus_tag="OQM83_002928"
     CDS             complement(1203..2744)
                     /gene="nhaB"
                     /locus_tag="OQM83_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCS1521136.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            2965..3684
                     /gene="fadR"
                     /locus_tag="OQM83_002929"
     CDS             2965..3684
                     /gene="fadR"
                     /locus_tag="OQM83_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCS1521137.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(3736..5268)
                     /locus_tag="OQM83_002930"
     CDS             complement(3736..5268)
                     /locus_tag="OQM83_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCS1521138.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5598..6896
                     /gene="dadA"
                     /locus_tag="OQM83_002931"
     CDS             5598..6896
                     /gene="dadA"
                     /locus_tag="OQM83_002931"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCS1521139.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            6906..7976
                     /gene="dadX"
                     /locus_tag="OQM83_002932"
     CDS             6906..7976
                     /gene="dadX"
                     /locus_tag="OQM83_002932"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCS1521140.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8363..10099)
                     /locus_tag="OQM83_002933"
     CDS             complement(8363..10099)
                     /locus_tag="OQM83_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCS1521141.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10194..11108)
                     /gene="ldcA"
                     /locus_tag="OQM83_002934"
     CDS             complement(10194..11108)
                     /gene="ldcA"
                     /locus_tag="OQM83_002934"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCS1521142.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11208..11819
                     /gene="emtA"
                     /locus_tag="OQM83_002935"
     CDS             11208..11819
                     /gene="emtA"
                     /locus_tag="OQM83_002935"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCS1521143.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(11821..12555)
                     /locus_tag="OQM83_002936"
     CDS             complement(11821..12555)
                     /locus_tag="OQM83_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCS1521144.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            12756..>12960
                     /locus_tag="OQM83_002937"
     CDS             12756..>12960
                     /locus_tag="OQM83_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCS1521145.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAV"
BASE COUNT         3288 a         3308 c         3406 g         2958 t
ORIGIN      
        1 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
       61 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      121 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      181 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      241 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      301 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      361 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      421 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      481 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      541 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      601 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      661 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
      721 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
      781 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
      841 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
      901 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
      961 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1021 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1081 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1141 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1201 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1261 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1321 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1381 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1441 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1501 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1561 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1621 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1681 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     1741 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     1801 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     1861 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     1921 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     1981 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2041 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2101 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2161 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2221 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2281 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2341 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2401 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2461 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2521 accgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2581 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2641 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     2701 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     2761 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     2821 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     2881 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     2941 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3001 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3061 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3121 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3181 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3241 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3301 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3361 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3421 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3481 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3541 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3601 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3661 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgggg
     3721 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     3781 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     3841 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     3901 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     3961 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4021 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4081 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4141 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatctggga accagtattt
     4201 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4261 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4321 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4381 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4441 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4501 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4561 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4621 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4681 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     4741 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     4801 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     4861 taagtaattg tttttaaaga aagagttatg cccatagcag gcgtgtgcca tcaccagcgc
     4921 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     4981 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5041 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5101 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5161 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5221 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5281 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5341 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5401 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5461 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc tgtatgttga
     5521 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5581 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5641 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     5701 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     5761 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     5821 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     5881 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     5941 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6001 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6061 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6121 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6181 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6241 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6301 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6361 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6421 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6481 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6541 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6601 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6661 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     6721 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     6781 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     6841 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     6901 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     6961 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7021 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7081 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7141 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7201 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7261 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7321 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7381 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7441 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7501 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7561 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7621 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7681 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     7741 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     7801 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     7861 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     7921 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     7981 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8041 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8101 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8161 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8221 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8281 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8341 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8401 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8461 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8521 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8581 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8641 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     8701 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa
     8761 atagtgccgc aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcacctacac
     8821 accatttatc ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat
     8881 ccagaccaac gcgtgacact ggacgtccga cgggcggaac caccactttg gcttttttcg
     8941 ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga
     9001 cattaaagaa cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga
     9061 ttggcaccgc gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac
     9121 cgcggaaggg gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg
     9181 ccgacaaaat gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc
     9241 ccagcaccag gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga
     9301 tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca
     9361 gaataccgct gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg
     9421 ccagcaatgg atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat
     9481 aaccaccgcc aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga
     9541 acatccaact gacactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa
     9601 agaccgccat tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt
     9661 taagcccctt accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag
     9721 cgccgattaa taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg
     9781 ttaaaccaga ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca
     9841 cacgaaagga actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca
     9901 ggttacttac catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga
     9961 ctcctgccag catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag
    10021 aaaatgaact aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa
    10081 ttattgttgt ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc
    10141 taccatatta gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt
    10201 ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg
    10261 gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta
    10321 atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct
    10381 gcgtcataat cattgggcgt gctgccgcta aagctaccga gaatgatcgc cttctgacgt
    10441 ggcaaaattc ccgcatgata gagctgcaac agcatacgtt cgtcccggaa agggtgctcg
    10501 ttaatatctt ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccgatc
    10561 agtgaaatca gcatcgcaag attgcctccc cacaacgtgc cttcggcccg acatgtcggg
    10621 ccttcccctt gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc
    10681 tcggtaaagg cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag
    10741 gtgatgacat tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgcccg
    10801 caaatgagca acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc
    10861 agtaaacgac tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtggtc
    10921 agtctggcaa gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca
    10981 caacgacggg caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgctgg
    11041 ataccacgca gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac
    11101 agagacatgg caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg
    11161 tgccgttgtc acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat
    11221 ggtttgcctt tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc
    11281 cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga
    11341 aagccggtgc agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat
    11401 cgggtggtaa tccgaatgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag
    11461 cttcaacctc cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca
    11521 gcgagctgaa aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg
    11581 aaaccggccc gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt
    11641 catacgctaa cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga
    11701 tcagcaaaat caacgatttg gatgctgacg agttcctcga acacgtagcg cgaaatcacc
    11761 ctgcgccgca ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa
    11821 tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg
    11881 ctgtaattgc cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt
    11941 ggtgatggtt tcattcttgc catcaatcac tttgcgctcg ctgatagaga ttaactgggc
    12001 gtcaaagtga aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc
    12061 ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa
    12121 atcatacagg cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg
    12181 cggatggagt ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg
    12241 aggcggtacg gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac
    12301 agtaaactcg actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag
    12361 cacagcgatg ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat
    12421 tgccagtatt ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc
    12481 tttgtgcaaa tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc
    12541 atggtaatga ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca
    12601 cagttaacaa aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc
    12661 cgacatacgc ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt
    12721 tcttgcgtcc gattcatcga aacgagggcc tgaacatggg aattattgct tggattattt
    12781 ttggcctgat agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat
    12841 ttttcctgac ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca
    12901 tgtttggcat tggcggctcc atcagcggtt ttaatttgca cagcttcctg gtggcggtgg
//
LOCUS       DAJVQG010000114        12409 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJVQG010000114 DAJVQG010000000
VERSION     DAJVQG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.114"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OQM83_002938"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OQM83_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCS1521146.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OQM83_002939"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OQM83_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCS1521147.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /locus_tag="OQM83_002940"
     CDS             complement(2659..5772)
                     /locus_tag="OQM83_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCS1521148.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5797..6954)
                     /gene="mdtE"
                     /locus_tag="OQM83_002941"
     CDS             complement(5797..6954)
                     /gene="mdtE"
                     /locus_tag="OQM83_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HCS1521149.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7014..7292
                     /locus_tag="OQM83_002942"
     CDS             7014..7292
                     /locus_tag="OQM83_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521150.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="OQM83_002943"
     CDS             complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="OQM83_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCS1521151.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="OQM83_002944"
     CDS             complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="OQM83_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCS1521152.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9446..9778
                     /gene="hdeA"
                     /locus_tag="OQM83_002945"
     CDS             9446..9778
                     /gene="hdeA"
                     /locus_tag="OQM83_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCS1521153.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            9894..10220
                     /gene="hdeB"
                     /locus_tag="OQM83_002946"
     CDS             9894..10220
                     /gene="hdeB"
                     /locus_tag="OQM83_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCS1521154.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10284..10931
                     /locus_tag="OQM83_002947"
     CDS             10284..10931
                     /locus_tag="OQM83_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCS1521155.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(10973..11503)
                     /locus_tag="OQM83_002948"
     CDS             complement(10973..11503)
                     /locus_tag="OQM83_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1521156.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11659..12225)
                     /gene="slp"
                     /locus_tag="OQM83_002949"
     CDS             complement(11659..12225)
                     /gene="slp"
                     /locus_tag="OQM83_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCS1521157.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3430 a         2753 c         2808 g         3418 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacatttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagttttttc gctaagaaac
     1501 agtctggcaa catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcat aaaatatcgc tgcataacga gagtatcgtg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctatta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct cataaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcaga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcga gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagca ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgcg ccgctcataa aggccatcgg cataaacact gcggaaagaa caacagcaat
     4441 accgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggtggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agttaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaatcgtcg ggatgattgt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgtttta aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggt agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgct ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccgga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca gattgtattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcagaatatt actgctcgac ttatcaacgc taatcccctg
     5401 ctgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cctggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacaacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgagtgac gccttcctgc ggcaccagta atacattctg
     6061 gcggctacct tcatccacta atgccgtgac gtacatgcca ggcagcaagt caccatttgg
     6121 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga
     6181 gaatttcagt gtgccggtct ggctgtagcg tttaccattt tccagattga gctgtactgg
     6241 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgcaagaa
     6301 atcctgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
     6361 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
     6421 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
     6481 cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg cggtgtcgta
     6541 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
     6601 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
     6661 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
     6721 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
     6781 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccggggg agagtgtgac
     6841 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
     6901 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
     6961 ccctgaaaat tcttgagaaa taacggtttt ttaaattgcg cgcgaggaga gatatgaggg
     7021 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     7081 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     7141 gcattcgggc aacgaagggt tctactggtg gatacacata cctggggaat aaagtcgttt
     7201 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     7261 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     7321 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     7381 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     7441 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     7501 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     7561 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     7621 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     7681 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     7741 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     7801 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc
     7861 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     7921 tttacccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     7981 acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt gtaaatagta
     8041 aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata accagtttgt
     8101 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     8161 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     8221 agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     8281 tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
     8341 tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa
     8401 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     8461 aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat tatttcaatg
     8521 atagtgcgca attgatctac aacactccgt agcggagaga gtattaatcg gatcatagtc
     8581 atatcaagtg actatgatcc gggtgacaac cgggataatt attgctgctt aacgaacaaa
     8641 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     8701 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     8761 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     8821 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     8881 atgcccagct ccggtgcgcg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     8941 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     9001 agcgcaatgc ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     9061 acgaacggga aactgataca cagcaacccg acgataaaca gtagcacggc aataaactgg
     9121 attgctctgc gatgtttttt aagcatctcc agatcaaact tcaaaattgt tgccttatct
     9181 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
     9241 ccaaaaacgc gtctaagaat gtagtcgatt taataaaaat ttcctaatta cagtatctga
     9301 tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt tatatcaaca
     9361 tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt gacggctctt
     9421 tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct
     9481 gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg ataacaaaaa
     9541 accggtcaac tcctggacct gtgaagattt cctggctgtg gacgaatcct tccagccaac
     9601 tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg cggttttaga
     9661 tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaca
     9721 agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt
     9781 ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata tcttctttaa
     9841 aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac aaaatgaata
     9901 tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga
     9961 acgcacaatc tgcgctggca gccaatgaat ccgctaaaga tatgacctgc caggaattta
    10021 ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat gaagaaacag
    10081 tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag
    10141 tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat
    10201 ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt
    10261 gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt atactcgcgg
    10321 caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac
    10381 gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg aaatatggtt
    10441 ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt gctgctcagg
    10501 tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac caaaatattg
    10561 taggtctgac gacggcagcg gatatctggg tgaccgccgc cataggtatg gttattggca
    10621 gcggtatgta cgaactgggt atttatggtt cagtgatgac cttgttggtg ctggaagtct
    10681 tccatcaatt aaccttccgc ctgatgaaca aaaattacca tttacagctg acattagtga
    10741 acggtaatat ggtatcaatg ctcgactggt ttaagcagca aaaaataaaa actgatttag
    10801 tttcgttgca ggaaaatgaa gatcatgaag tggtcgccat tgatatccag cttcatgcca
    10861 caacgtcgat agaagacctg ttgcgattat taaaaggtat ggcaggggtg aaaggcgttt
    10921 caattagcta atctgtgtgg cagcgtagcc agactcaccg taagcctgaa attcacacca
    10981 gataatcaat atgctgatgg cgtaccagct cattgatccg cttaagattg aggatcatca
    11041 tgatattgtg tttatggcag tagaacgttt ttagcgatat tttcagtttc tccagaatat
    11101 cttcagttgt catttgattc gcaatgcagc tcagaacctg atgttgcttg cgcgaaagtg
    11161 acacattcgc ttcaggcaca gagcagtaac tgccattgat gagtgcgaca aaatcaatga
    11221 tagtgatagt ccgcggaaca aaggtcaccg gtactttccc cagacagcgt ttaatattaa
    11281 agggggagaa aacaatgaca tggacaggtt ttaatcgttc aatttgagtg agaaaattag
    11341 aatgaaatac attattcatt aatgtatcaa taatgacgaa ggcattctga tgcaacatta
    11401 cgtcgatctc ttcttcgttc tgtatatgaa cgacattacc tttactcaga atgtttttca
    11461 tcgcggtgaa gaacatcgta tccctggtaa ttataagaaa cataaccgac tcctggtgcg
    11521 gacattaact ttataatcaa tttgccaaag gtaattaaca ggacagggct tataataata
    11581 atagttagct tactgtatat taattttatg taatacatga tgcagatgct gagaaagcat
    11641 ctgcatcttt cggtggtgtt atttgaccag ctcaggtgtt acctgactca ccgcattggt
    11701 gtagtaaggc gcaccccagc ccggttccgg ccagaatgca ccatagccgt aatcccacag
    11761 gttataggtg gtattaacca cttctctcaa atgccacacc tggatgccct gcatattcac
    11821 ttccaggaag ttatacggga ctttattgat aaagccaggt tgttcaccct gaatggtacc
    11881 gaggatggta acaaagtgat tacgatagtt cactgggtca aggaagccgc tttgtctggc
    11941 gagcagtcgg ccctgatagt tggcttcaat atcaggtttc gcatagctat ccaacggtaa
    12001 tacagcgatt tctaacaacg tatccgtttt gccattgata acgttgataa ccttcccacc
    12061 aaagcgcgct tgttgaccaa catataaccc cggctggtta tgaacagcaa caaaactttt
    12121 ttgaatatca ggttggttat tgcctttgat attttgcgga attgaactac atgcggcaag
    12181 caaaaatgaa aggctgagga tgagtgcacc ttttgtcatg ttcatgttac tatccttatc
    12241 aacaaatatt aaaaccataa tagatatttc tttatagttt tcatctgatt ctgaggcgat
    12301 atgcctgata gtaacgtaaa cctgtctgat gtttatatcc cttttgttta gttcaattcc
    12361 attgattttg gggcgtggca tgagtatcca tcgccccata atataatcc
//
LOCUS       DAJVQG010000115        12230 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJVQG010000115 DAJVQG010000000
VERSION     DAJVQG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12230
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.115"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..201)
                     /locus_tag="OQM83_002950"
     CDS             complement(<1..201)
                     /locus_tag="OQM83_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HCS1521158.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFD"
     gene            complement(403..1047)
                     /gene="cheZ"
                     /locus_tag="OQM83_002951"
     CDS             complement(403..1047)
                     /gene="cheZ"
                     /locus_tag="OQM83_002951"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCS1521159.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1058..1447)
                     /gene="cheY"
                     /locus_tag="OQM83_002952"
     CDS             complement(1058..1447)
                     /gene="cheY"
                     /locus_tag="OQM83_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCS1521160.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1462..2511)
                     /gene="cheB"
                     /locus_tag="OQM83_002953"
     CDS             complement(1462..2511)
                     /gene="cheB"
                     /locus_tag="OQM83_002953"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCS1521161.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2514..3374)
                     /gene="cheR"
                     /locus_tag="OQM83_002954"
     CDS             complement(2514..3374)
                     /gene="cheR"
                     /locus_tag="OQM83_002954"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCS1521162.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLMDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHAHRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGYSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3393..4994)
                     /gene="tap"
                     /locus_tag="OQM83_002955"
     CDS             complement(3393..4994)
                     /gene="tap"
                     /locus_tag="OQM83_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCS1521163.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5040..6701)
                     /gene="tar"
                     /locus_tag="OQM83_002956"
     CDS             complement(5040..6701)
                     /gene="tar"
                     /locus_tag="OQM83_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCS1521164.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6846..7349)
                     /gene="cheW"
                     /locus_tag="OQM83_002957"
     CDS             complement(6846..7349)
                     /gene="cheW"
                     /locus_tag="OQM83_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCS1521165.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7370..9334)
                     /gene="cheA"
                     /locus_tag="OQM83_002958"
     CDS             complement(7370..9334)
                     /gene="cheA"
                     /locus_tag="OQM83_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCS1521166.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9339..10265)
                     /gene="motB"
                     /locus_tag="OQM83_002959"
     CDS             complement(9339..10265)
                     /gene="motB"
                     /locus_tag="OQM83_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCS1521167.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRVIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10262..11149)
                     /gene="motA"
                     /locus_tag="OQM83_002960"
     CDS             complement(10262..11149)
                     /gene="motA"
                     /locus_tag="OQM83_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCS1521168.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11275..11853)
                     /gene="flhC"
                     /locus_tag="OQM83_002961"
     CDS             complement(11275..11853)
                     /gene="flhC"
                     /locus_tag="OQM83_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCS1521169.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11856..12206)
                     /gene="flhD"
                     /locus_tag="OQM83_002962"
     CDS             complement(11856..12206)
                     /gene="flhD"
                     /locus_tag="OQM83_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCS1521170.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         2792 a         3422 c         2988 g         3028 t
ORIGIN      
        1 atcaaaatgc agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc
       61 aaaccagata acacacacgc ccactaacaa aatcagcaac gaggtcagtt cacgggaacg
      121 cgggatttgc ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt
      181 gtcgtcgctc tcgtcagaca cgtcgccaat cctggaaaga gtattaagct taaatgatgc
      241 cagagcgcaa agcgttcaat ggtttgagta aggggcaaaa caggcgggat ttagggcttt
      301 tgctgccaca catcaagcat agtgtgcgtt tgtcggatgc ggcgtcatcg ccttatcaga
      361 ccgcctgata tgacgtggtc acgccacatc aggcaataca aatcaaaatc caagactatc
      421 caacaaatcg tccacctgat cctgactggc taccacgccg actttgctgg tatcgacctg
      481 aggtccatta agcaaactct ggttttcacg ttttggacgc gactcctgtt ccgggatgtt
      541 ttccaacagc accatcagca actggcgttc gatctcctga atgacatcca tcatccgctt
      601 aatgaccagc ccggtgagat cctgaaaatc ctgcgccatc atgatttcca gcagttgcgc
      661 gttagtaaag ctggtatgcg cgggtacatc tgccagaaat tgtcgtgtat ctgttaccag
      721 ttcacgggca tcggcaaggt caatcggatc ggcaaaccag tcatcccaac gttgggttaa
      781 cgcttttgct gatttctcca tttgatcctg atgcggttgt gacgcctcaa cactgttcag
      841 cgcccgctcc gcagcctggg cggtcatctg cacaacatag tacaaacgat cgcgcgcatc
      901 ggggatggct tccgccgctt cggcaatggc ctgatccagc cccagttccc gcaaactgtc
      961 gcgcagcata cgcgtcaggc tgccgatgcg cgcaatgata tcgccagctg aatgctcgtc
     1021 agcaggtttg attgatggtt gcatcatagt cgcatcctca catgcccagt ttctcaaaga
     1081 ttttgttgag tttttcctcc agcgtcgcgg cggtaaatgg cttcaccaca tagccactgg
     1141 cccccgcttg cgccgcagca atgatgttct ctttcttcgc ttctgcagtc accattaaca
     1201 ctggcaatgc cgacatcgcg ccatccgcac gaattgtttt cagcaattcc aggccatcca
     1261 tattgggcat gttccagtcg gagataacaa atccataacc gcctgcctgc aacttattga
     1321 gagcgtcgac gccatcttcc gcttcctcaa cattattgaa tcccagctct ttcagcaggt
     1381 tacgcactat gcgtcgcatg gtggaaaagt catccacaac caaaaattta agttctttat
     1441 ccgccatttc acactcctga tttaaatacg tatcgcctgt ccggcactaa tttttgccaa
     1501 catttgctgg cttacctggc taagatcgac cacttcgcag acaccaccca tattgatggc
     1561 ctcgcgcggc atgccgaaca ccacgcaact tgcttcgttt tgcgcaaggg tccatgcccc
     1621 cgcctgacgc atcgccaaca ttcccgccgc gccgtcgttg cccataccgg tcaggatcac
     1681 cccaaccgca ttacgccccg cctgtttggc gacagaatgg aacaacacat ctaccgaagg
     1741 ccgatgacgg ttaaccgccg ggccgtcgtg aattttgatt tgataatttg cgccactacg
     1801 cgccagttcc atatgccgat cgcccggcgc aatataggca tgccccggca agacacgttc
     1861 tccgtcttcg gcttctttaa ccccgatctg gcaaagctta ttaagtctgt cggcaaaaga
     1921 gcgggtgaaa ccgggcggca tatgttgggt aattaacaat gccgggctgg aaagcggcaa
     1981 cggttgcagt acgtgacgaa ttgcctcagt tccacccgtt gaagcaccaa tcgcaatcag
     2041 tttttcagaa ctcaacaacg gacccgcctt cagcgttgtc ggtgctgaca atggcttatg
     2101 tgctgcaagg ctcgcctttg ctgccgtacg caccttttca gcaatcattt cgctatacgc
     2161 cagcattcct tcgcgaatac ccagttgcgg tttggtgaca aaatctattg cccccagctc
     2221 cagcgcgcgc agcgtgactt ctgatccttt gccggtcaag gaagaaacca tcacaacggg
     2281 cattggacgc aaacgcatta atttttcgag gaaatccagt ccgtccatcc gcggcatttc
     2341 aacatccagc gtcagcacat cgggattgaa tttcttaatc aagtcacgcg cgaccagcgg
     2401 atcaggcgcg gtcgccacca tttccatgtc gctatggctg ttgatgattt ctgtcatgat
     2461 ctggcgcatc agtgccgaat catcgacaga taacaccctg attttgctca tcgttaatcc
     2521 ttacttagcg catacaccgt ctgaccacgc agcgtgaagc ggcgctcaag gtggctaaag
     2581 ttttcagagt aacccgcaaa cagcaatccg tcgggtttaa ggagcggaac aaagcggcgc
     2641 aaaatctcct gctgggtatt ttgatcgaag tagatcatga cgttacgaca gaagatcgca
     2701 tcaaacggcc ccggcacggt gtactgtttc gccagtagat tcagcggggc aaaatcaaca
     2761 tagttcgcca gctcctgacg cacgcgtacc agcccttcat gcggtcccgt ccctcgcatg
     2821 aaataccgtt gcagttgctg cggcgtcagg tttttcaact cttcatggcg ataaataccg
     2881 cttctggctt tttccagcac ttcggtgtcg atatcactgg caaacacttt ccagcgtccg
     2941 ggcgcggtgc ccaatgtgtc agccagcgtc atcgcaatgc tgtacggctc ttcgccggtc
     3001 gaagccgccg cgctccatac gcgatactcg ccagaacggc gatgtgcgtg atccgcgagc
     3061 agagggaaat ggtgtgcctc acggaaaaat gccgtcagat tcgtggtcag cgaattgata
     3121 aacgcctgcc actcaccgct gtgctgatta gattccagca agttcagata atgaccgaaa
     3181 tccatcagtc ccagcgaacg caaacgacga accagtcggt tgtaaaccat gtcgcgttta
     3241 tggtcagcca gaacgatccc ggctcgttga tagatcaatt gacttatccg ccgaaaatgc
     3301 gcgtcggaaa gcgccaggcg ctcggtcatc tgtaacaata aagacgtttg cccacagggc
     3361 agagatgaag tcatagcgcc ttctcaatca cttcaggata ccactggcgc aatttgtaac
     3421 tgcgccgact catgtcgtgc tacttcatgt tcttcaaggg taaataccgc cacacgcgac
     3481 gaaagatggt cggcctgatt cgccagttgt tccgttgcca ccgccgcttc ttctaccagc
     3541 gaggcgttct gctgagtcac ctgatccatc tggctgacag cctgtgcaac ctgctcaatc
     3601 ccccgctgtt gttcttccga cgcagaggca atttctccca taatgtcgtt cacgcgggtc
     3661 accgaactga caatatcgat catggtcgcg gcggcgttat tcaccagttt cgaaccctgc
     3721 tggacacgat tgactgactc ttcgatcagc cctttgatct cttttgctgc ctgcgcgcta
     3781 cggctggcaa gattgcgcac ttcacctgcc actaccgcaa aaccacgccc ctgctctccg
     3841 gcgcgagccg cttccactgc cgcattcagg gccagaatat tggtctggaa agcaattccg
     3901 tcgataacgc tgataatgtc gccaattttt tgcgagctgg tggcgatctc ctgcatggtg
     3961 tgagtcatgg tactgacctg aacaccgccc gcctgcgccg ttgtcgcggc attttttgcc
     4021 agttccgacg cctgtcgtgc gttatcggcg ttttgcccta ccgtggcggt taattgctcc
     4081 atactggcgg ccgtttgtgc cagcgatgcc gcctgctgtt cggtacgact tgagagatcg
     4141 ttattgcctg cgacaatctc cgcgatacca atgtgcatct cctggcttcc cttacgcaca
     4201 tcacttaccg tcccacgcaa agcctgctgc atggtcttca gactggcaaa aatggcggtg
     4261 atctcattac gaccatatac cgcaatcgga cgcgccagat tacccgcagc aatgctgtca
     4321 aaatggctac cgataatggc cagtggttga acaatcatct tgcgcgtcca ccacagcgca
     4381 ctgctgatgt agatcgccgc aacaataatc atgctgataa acaccagtgt cgaaatctga
     4441 tagttacgct ggctttgcgc actggcggct tccagcacat ggttgatctc cagctgccag
     4501 gcctcaaagt taacgtcaaa cgcattctgt gatccctgca ccggcgcagt gaggaaatcc
     4561 gaaagttgat tactttcaag ccaggtcgcc tgatgttcga gatcgttgtg ccagcgggca
     4621 aaacttttct ccgtctcttt ttgcaatgcc ctgacgtgct cgttgcccgc agtcatcgcc
     4681 ataaaacttt taaacagcgt ggtggattgc gtcagactgg cgcgcgccgt cgtcatcaac
     4741 gttttaatgt catccgccgg atagctaagt gccgtcagag tgcccgcttt gttcagcgcg
     4801 gtactggcct gtaacattac cgcccgagtt tgcgctaatg ccgcacgttg ctgattgctc
     4861 tgctcgacct gattcagtcg ttgcaaatcg tcgcgaaatg cccaaaaaga catgccgtta
     4921 ctgccaatct gcaagatccc gcagagaatc aaaattaaaa acagcgtggt cgaaattcga
     4981 atacgattaa acatcaacgc tccccatcag gcggcaatga ccgcgttagt aaatactcgt
     5041 caaaatgttt cccagtttgg atcttgttca gcaattcgca gtcgtggctg tgccggtggt
     5101 tgctcactgg caggacggga tggtgtttgc ggtttattgg tgagtgggct ggctgccaga
     5161 cggaacgcgg aaaccgcttg cgttaaacga ctcgcctgtt cttccagcgc agcggcggcg
     5221 gcagctgatt cctgcaccag cgatgcgttc tgttgcgtga cgcgatccat ttccgaaacc
     5281 gccaatgcga cttgatcgat gccacggctc tgttcatccg atgccgatgc aatctcgccc
     5341 ataatgtcag tcacgcgagt gacagcattg acgatattgt tcattgtttc cccggcgctt
     5401 tcgaccagca ccgaaccggt atcaacgcgt gcgacggagt cttcaatgag ggctttgatc
     5461 tcttttgccg cctgggcgct gcgactggca agattacgca cttcacccgc caccacggca
     5521 aaaccacggc cctgttcacc cgcacgcgcg gcttcaaccg cggcattcag cgcgaggata
     5581 ttagtctgga aggcaatacc gtcgataacg ctgataatgt cggcaatttt cttcgaacta
     5641 tcggcgatct catgcatcgt tttcactacg ccatccacca ctttgccgcc gtgctgggcg
     5701 gtgtcggagg cactttgcgc cagttgcgag gcctggcggg cgttatcggc gttttgcttc
     5761 actgtcgcgg tgagctgctc catgctggcg gcagtttctt ccagcgcgga tgcctgctgt
     5821 tcagtacggg aggaaagatc ggtgttgccc gccgcaattt cacgggtacc ggcatagatg
     5881 gcatctgaac cttcgcggac atgagtgacg gtgtcagtca aagagcgttg catatgtgaa
     5941 acgctctgcg ccaggtcgcc catttcactg cgcccgtcaa tggtcagggt attcgctagg
     6001 ttaccaccgg cgatttcgcg aatgtgagca ataatttttg ccagcggagt aagcaacata
     6061 cggcgaatgc cgtaccacgc caccagcaga atcaatacca ccaccagcgc gataaccgcc
     6121 agttgccact gggcaaatcg gtaatcatct gcgttgtcag tgacgatatc gcgatacagt
     6181 ttttcactgc tgagggcgta ctgagcaaac gcttcgccca ttgcattttg cattccctgg
     6241 gttggctgag cgaaataagc tccagtattg ccataatcta gataatcaat cagttcagtt
     6301 aacgctgtgt aatagttttt atatttttca tcaatattac gactggtagc gaccatttca
     6361 ggtaacggtg ccatgctttt gaatttttta taatgcgtcg ctgcctgcgc caatgttttc
     6421 ctggcgctat cgagcaattc aactttggcg ttactttgtt gattggagga atccatcatc
     6481 atccgcaccg ctgaacgact caggttaatg cgcgtttgca gcattaaatc ccaggttgac
     6541 gtcagctcgc cctgctgttc ccgcaattga ttggaaacca caaagctctt ctggctatgg
     6601 tgaagggaag aaaaaaacag actgccggaa ataagctgta acagtgcgaa tacccccagc
     6661 accattacca acagcgtgac tacgcggata cggttaatca taaggcacct tcctgaaaac
     6721 aagttgatct cgttatcggc aagaaggggg gaaactttat tgctgatgcc acccgccgcg
     6781 aaattgaaat aaaaaacccg atgcgcagat catcgggttc atttcaattg aggaaatcgg
     6841 gagaattacg ccacttctga cgccgcgcta tctaacagcg ccatctcttc gctgttcagc
     6901 agtttttcga tgttcaccag aatcaacata cggtcgccca gtgcgcccag tccagtgaga
     6961 tattctgttg aaagcgtcac ggcaaactct ggtgccggac gaatttgctc cgccgtcaat
     7021 gaaagcacgt ctgaaacgcc gtcaaccacg atgccgacca cccgctgtcc gagattcagg
     7081 acgataacta ccgtgttgtc gttatagtcc acatccacct ggctgaactt aattcgtaag
     7141 tcaacaatcg gcacaataac gccgcgcaga ttcgtgacgc ctttgataaa cgctggcgtg
     7201 ttcgcaatcc gtgttacctg atcgtagcca cggatctcct gcactttcag gatatcaata
     7261 ccgtactctt catcaccaag ggtaaatacc agaaattcct ggcctgacgg ctcgctggcc
     7321 agctttgtta cattcgtcat accggtcata ttgttacctt tttactcatt caggcggcgg
     7381 tgttcgccat acgttgttcg cggtttatcg cctgcaaggc ggagacatca acaatcagtg
     7441 ccacgctgcc gtcgccaaga atggtcgcag cagaaatgcc ggggactttg cgatagttac
     7501 tttcaaggtt tttaaccaca acctggtgtt gaccaattaa ttgatccacc agcaaggcgt
     7561 agcggcgacc gccactttgt aagatcacaa caattccctg ggtggcttcg gttttcgcgc
     7621 ccgcgacgtt gaacactttc cacagttcga cgatgggcag atattcaccc cgcacttcca
     7681 gtacccgctc gccgccggcc agtggatgga gatcggcttc acggggttgc agtgattcca
     7741 taacagcatt cagcggcaga atgaaaactt catccccaac gcgtacggac atgccgtcga
     7801 ggatggccag cgtcagcggc agtaaaatgc ggatcgtagt gccagtcccc tgcttcgact
     7861 ggatttcgac atgaccgccc atctcctgga tattacgttt aacgacgtcc atgccgacgc
     7921 cgcgcccgga gacgtcggtg acctgctctg ccatggagaa gcctggtgca aatatcagca
     7981 tcgcgacttc gtcgtcgctc atgttttcgc tgacagtcaa accttgcgag gccgcttttg
     8041 ccagaattcg ctcacggttt agccccgccc catcgtcggt cacttcaatg caaatgttgc
     8101 cgccctgatg ttcggcagac agaattaaat ttccgacgct gtttttacct gcggcgagcc
     8161 gtttttctgg cagttcaata ccgtgatcga ggctattgcg taccaggtgg gtcagcgggt
     8221 cgataatgcg ttctatcagg cttttgtcga gttcagtaga actgcccacc agcgtcagtt
     8281 ctacctgctt gccgagtttt cccgccagat cacgcaccag ccggggatag cgactaaaaa
     8341 catattccat cggcatcatg cgaatcgaca tcactgattc ctgcaaatca cgggcgttac
     8401 gttgtaactg ccccatgctg gttatcaaat caccatgatt aaccgggtcc agttcgctgg
     8461 aacgctgggc aagcatggac tgggtgataa ccagctcgcc gacgaggtta attaattgat
     8521 caaccttttc taccgctaca cggatgctgg tggattcatt gctgcgcgtc gttttttccc
     8581 gctccacgcg gccggttggc gcttgttcgg ctgccagttt aagcactggt ggggtggata
     8641 tttttggcga gacttctact gtttcaaagg taatctgatc ggcttcaatc acaaaacaga
     8701 gtaccgctgt gatgtcatct tcggcgatgt cgcccggtaa tattgccgag agcgaatccg
     8761 cccctttcac cacgtcagtt aacgttgtca gatgtcccag ttcttcttcc agcaggtcga
     8821 cttccccggc cttcaggcgc gaaaggataa ttcgtcgcgg cgactgactg cgactctgct
     8881 catcttgcgg ttcacttttg gcaaccacac ttaatcgggt cactgcggat ggcgtttcgc
     8941 ctttcgcttc taatgccagt tgacgcaagg cctggcagat ataatcgaag ctggcggcat
     9001 ccggctcttg cgactgttta taagcgtcga gctgttcttg catgatgtcc ttcgtttcca
     9061 aaaacagatt gataatgtcg gtgttgagtt gcatctcacc tcgtctggct tcatcgagca
     9121 ggttttccat cagatgcgtg gtttcctgca aaacgctgaa gccaaaagtt cctgcccctc
     9181 ctttgatcga gtgggcagcc cgaaagatgg cattcaattg ttcggcatct ggcgcttccg
     9241 gctgcaaaac cagcagatgc tgctccatgt cagccaacag ttcgtccgct tcatcaaaaa
     9301 atgtctgata aaaatcgctt atatccatgc tcacgctgtc acctcggttc ggctgatggc
     9361 attgtgggaa cactgacctg tggtgcaacc tcaggttttt ccagggcgct tactggctca
     9421 ttctggcttt cggcgttttc atgcaaaatg gcctgttcgg cttgtttgtt cagtaccagc
     9481 aggctgatgc gacggttgac ggcatcatca ggtccgcgat cgcttaagcg catcgttgcc
     9541 gccatgccga cgacacgtaa cactttgccg ctatccaacc ctccgaccat cagttcgcgg
     9601 cgggatgcat tggcccgatc ggcagaaagc tcccagttgc tatatccttt ctcaccgctg
     9661 gcgtagggga aatcatcggt atgacctgaa aggctaatac ggttgggaat accgttcagt
     9721 acaggcgcaa tgacacgcag aatgtcgcgc atatagggtt cgacatcggc actgccggtt
     9781 ctaaacatcg ggcgattctg gctatcgatg atctgaatac gtagaccttc ctggaccaga
     9841 tcgattttga gatggggacg taacgcccgc agtttcggat cggactctat caactggtcg
     9901 agatcacccc gcaatttccg caatcgactt tgctccatgc gttttttcag atcttcgatg
     9961 ttcggctgct tattcacttc cccctggctt tgggtgtaat catcaccacc gccgggaatt
    10021 gggctttcac tattagaaat gcgatcgccg cccgtaaccg cagtcgccag tggagtccgg
    10081 aagtactccg caatctgaat cagctctttt gggctggaga tggagatcag ccacatcacc
    10141 agaaaaaagg ccatcatcgc agtcataaag tcggcataag caatcttcca cgatccatgt
    10201 gctgccccgt ggcttttggc tttgcgtcgt ttgacgacaa taatcggatg cgcttgattc
    10261 ttcatgcttc ctcggttgtc gtctgttgtt gcggattttt caccgcacgc acatgctctt
    10321 ccagttcaat aaacgacgga cgttcgctgg aatagagcgt tttgcgacca aactcaacgg
    10381 cgataggcgg tgcgtaaccg ttcagattag aaagcagagt gactttgacg cactgcatca
    10441 ttttgctggt ttcggcgctt ttctgacgta aaacactcgc taatggggaa ataaatccgt
    10501 aagccaataa aatgccgagg aaagtcccca ccatcgcatg tgcgataagc gcacccagct
    10561 cggcggcagg acgatcggct gaacctaacg cgtgaacgcc ccccattaca gccgcgacaa
    10621 taccaaacgc cggaagtgag tccccgacca gcgccagact gttcgccggg acttctgcct
    10681 cgctttcgtg cgtctcaatc tcttcatcca tcagggcttc gatttcgaag gtgttcatat
    10741 gaccgctgat aatcagacgc agataatcga cgataaaatc aagcatgacg ctatccgcga
    10801 ggatgcgtgg gtagctggcg aagatctcgc tctcacgggg attttcaata tcacgttcca
    10861 gcgaaaacat ccccatctgc cgcgatttcg ccatcaaccg ataaagcaga gccagcagat
    10921 ccatatacat tgctttggtg tatttggagc gacgaaacag caacggcagc gccttcagcg
    10981 tgcctttaat cgctttgcca ttattgccga cgataaacga cccaatccct gcaccggcaa
    11041 taatcaccag ttcagcgggt tgatagagtg ctccaaggct tccaccggtc atcaaataac
    11101 cgccgaaaac tgtaccgaga acaaccaggt aacctaataa gataagcacg acatcatcct
    11161 tccactgttg accatgacag gatgttcagt cgtcaggcgt taacgcgcga ttggggcaaa
    11221 aaaagcagcg gtacgtcgtt accgctgctg gaatgttgcg cctcaccgta tcagttaaac
    11281 agcctgtact ctctgttcat ccagcagttg tgggataata tcggcaggat tctgggaaag
    11341 tttacgtctt tttactgccc gggatggcgg ttgacataag ctgcaggcaa agctgccaac
    11401 aggctggtga gcgtgggtaa taaaattgcc gccgcagcag ttgcagctgg aaagttgcag
    11461 taatccactt tcaacaaacc gcaccaatgt ccaggcacgg gttaatgcca gcagtggtcc
    11521 ttcttctgct tgtgggcact gttcaaggta taaacggtag gctttgatca ccgcatcgac
    11581 gccattacac aaaccggttt tcagtaaaaa ctgccatgca ttacagaaca tcgaagcatg
    11641 aacgttttgt tcccaggtca taaaccagtc ggttgagaat ggcagcatgc ctttcggcgg
    11701 tgggcttccg cgcagttctt tataaagttt tatcaggcgt ccgcgactta actgtgtttc
    11761 gctttccagc atctgcaaac gagcgcccag ggtaatcaat tccattgcca gctgaatatc
    11821 ccgcgcttcc tgaacaatgc ttttttcact catgatcagg cccttttctt gcgcagcgct
    11881 tcttcaggct gattaacatc attcagcaag cgtgttgaga gcatgatgcc ggtatgaatt
    11941 tgctggagat cgtcaacgcg ggaatcttgc gtcaactgag taatcgtctg gtggctgtca
    12001 aaacggaagt gacaaaccag ttgattggtt tctgccagct taaccatttg cggaagagtc
    12061 agtgccgcta acgttgtcgc catttcttca tttatgccga gacgaaacat agcggacgct
    12121 ttgtcctgaa caatcaaacg ctgtgcaagt agtaaatatg acaagttgat gtcataaatg
    12181 tgtttcagca actcggaggt atgcattatt cccacccaga ataaccaact
//
LOCUS       DAJVQG010000116        12042 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJVQG010000116 DAJVQG010000000
VERSION     DAJVQG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12042
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.116"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(78..1217)
                     /gene="nlpD"
                     /locus_tag="OQM83_002963"
     CDS             complement(78..1217)
                     /gene="nlpD"
                     /locus_tag="OQM83_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCS1521171.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(1357..1983)
                     /gene="pcm"
                     /locus_tag="OQM83_002964"
     CDS             complement(1357..1983)
                     /gene="pcm"
                     /locus_tag="OQM83_002964"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCS1521172.1"
                     /translation="MVSRRVQALLEQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(1977..2738)
                     /gene="surE"
                     /locus_tag="OQM83_002965"
     CDS             complement(1977..2738)
                     /gene="surE"
                     /locus_tag="OQM83_002965"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCS1521173.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(2719..3768)
                     /gene="truD"
                     /locus_tag="OQM83_002966"
     CDS             complement(2719..3768)
                     /gene="truD"
                     /locus_tag="OQM83_002966"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCS1521174.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(3765..4244)
                     /gene="ispF"
                     /locus_tag="OQM83_002967"
     CDS             complement(3765..4244)
                     /gene="ispF"
                     /locus_tag="OQM83_002967"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCS1521175.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(4244..4954)
                     /gene="ispD"
                     /locus_tag="OQM83_002968"
     CDS             complement(4244..4954)
                     /gene="ispD"
                     /locus_tag="OQM83_002968"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCS1521176.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(4973..5284)
                     /gene="ftsB"
                     /locus_tag="OQM83_002969"
     CDS             complement(4973..5284)
                     /gene="ftsB"
                     /locus_tag="OQM83_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCS1521177.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(5478..5801)
                     /locus_tag="OQM83_002970"
     CDS             complement(5478..5801)
                     /locus_tag="OQM83_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCS1521178.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(5851..6456)
                     /gene="cysC"
                     /locus_tag="OQM83_002971"
     CDS             complement(5851..6456)
                     /gene="cysC"
                     /locus_tag="OQM83_002971"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCS1521179.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(6456..7883)
                     /gene="cysN"
                     /locus_tag="OQM83_002972"
     CDS             complement(6456..7883)
                     /gene="cysN"
                     /locus_tag="OQM83_002972"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCS1521180.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(7885..8793)
                     /gene="cysD"
                     /locus_tag="OQM83_002973"
     CDS             complement(7885..8793)
                     /gene="cysD"
                     /locus_tag="OQM83_002973"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCS1521181.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            9045..10082
                     /gene="iap"
                     /locus_tag="OQM83_002974"
     CDS             9045..10082
                     /gene="iap"
                     /locus_tag="OQM83_002974"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCS1521182.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(10350..10643)
                     /gene="cas2"
                     /locus_tag="OQM83_002975"
     CDS             complement(10350..10643)
                     /gene="cas2"
                     /locus_tag="OQM83_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HCS1521183.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(10640..11563)
                     /gene="cas1e"
                     /locus_tag="OQM83_002976"
                     /pseudo
     CDS             complement(10640..11563)
                     /gene="cas1e"
                     /locus_tag="OQM83_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
     gene            complement(11560..>12042)
                     /gene="cas6e"
                     /locus_tag="OQM83_002977"
     CDS             complement(11560..>12042)
                     /gene="cas6e"
                     /locus_tag="OQM83_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCS1521184.1"
                     /translation="RFFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTIC
                     KAGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQ
                     QQLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA
                     "
BASE COUNT         2879 a         3320 c         2952 g         2891 t
ORIGIN      
        1 cgtattctga ctcataaggt ggctcctacc cgtgatccct tgacggaaca ttcaagcaaa
       61 agcctggttc cgccgattta tcgctgcggc aaataacgca gcgggtttac ggatttcccc
      121 ttgtaacgaa tttcaaaatg caagcgtgtt gaactggttc cggtgctacc catggttgct
      181 attttttgcc ccgccttcac ttcttgttgt tcccggacca gcattgtgtc gttatgggcg
      241 taggcactca ggtaatcatc attatgtttg atgataatca gattaccgta gccgcgcagc
      301 gcgttaccgg cataaacaac gcggccatct gcggtcgcga taattgcctg tcctttgctg
      361 cctgcgatat caatcccctt gttgcccccc tcagaagcgc caaaggtttc gatcactttg
      421 ccctcagtcg gccagcgcca ggtggagata ggcgtactgg ttgatgtact gctgacagtc
      481 ggctcggttg tgcttgctgt tggtaccgtt acaggcgctg tgactgtggt cgcagttggc
      541 ttgttgttcg gcaacatttt gttagcactc tgttcacccg aagactcaga atacgtaatt
      601 gtcggttgcg acgcaacagc aacggtggaa ttttgtgcag gcttgatcac aactccttgc
      661 tctgctgcgt cggcctgggt aatggcattt ccgccagtga ttggcgtacc ggaagcatta
      721 cccacctgca aggtctgacc aacgttcagc gcgtatggtg cctgaatatt gttgcgctga
      781 gcaaggtcac ggaaatcgtt gccagtaatc caggcgatat agaaaagtgt gtcgcctttt
      841 ttcacggtat aggtactgcc gctataactg cctttcggaa tgttcccata ctgacggtta
      901 tagacgatgc gtccgttttc catctgtacc ggctgctgag ctactggctg cactggctgg
      961 atttgcggtt gttgagtagc ctgaatttgt ggctgctgta ccggctgaat ttgcggttgc
     1021 tgggctgtag acgtcgtccc cattttcggc ggcggcgtaa tcaacatacc agaattagta
     1081 tttgcaggcg cattgccatt aacggagctg accggtgccg gtggatttga agtgtcagaa
     1141 cagcctgcca gccatagcga aaccagtgac aaagcagcaa tgcggcgaac ggtgaatttt
     1201 gggcttcccg cgctcattta tcccccagga aaaattggtt aataaccagt gacataatta
     1261 ccgtgcaagg caccctactg aacactggaa aagatgttca cgatacgctg acctgcggca
     1321 aaataaccag gaaaaatcca ggtatttcct cacgttttaa gccagctcac ccttcactaa
     1381 agggacaaag cgcacggcct ccacggtatc gataataaat tcgcctcccc gacgacgcac
     1441 ccgtttcaaa tactggtgct cctcccctac gggtaagacg agaatcccgc cttcgtccag
     1501 ctgcgtcatt agcgcagttg gaatttccgg cggtgccgcc gtaacaatga tagcgtcaaa
     1561 cggcgcacgt gcctgccaac cttgccatcc atcgccatga cgggttgaaa cattatgtaa
     1621 atcaagattt ttcaggcggc gacgtgcctg ccactgcaag cctttaatcc gttcaaccga
     1681 gcaaacatgc tggacaagat gcgctaggat tgccgtttga tatcccgaac cagtgccaat
     1741 ttccagcacc cgcgactgcg gcgtcagctc gagtaattcg gtcattcgcg ccaccatata
     1801 cggctgcgaa attgtctgcc cctgacctat cggcaaggcg atattgtccc aggctttttg
     1861 ttcaaacgct tcatcaacga atttttcacg cggcacggcg gcaagtgcat tcagcacctg
     1921 ctcatcctga ataccttgcg cacgtaattg ctccagaagt gcttgtacgc gtctgcttac
     1981 cattgcgtgc caactcccac gctgtttaac cagtctgaaa ccacatcttg cgcgctatgc
     2041 gcagttaaat ccacatgcag cggcgtgatg gagacatagc cctcatctac cgcagcaaaa
     2101 tcggtccccg gaccggcatc acatttaccg cccggcgggc caatccagta cagcgtattg
     2161 ccgcgcggat cttgctgcgg gatcacctga tctgccggat gtcgtgtacc gcagcgcgtc
     2221 acgcgaatac ctttgatttg atccaagggt aaatccggaa cgttaatatt aagaatacgt
     2281 ccggtgcgca gcggctcttt acacagtgcg cgcaaaattg aacaggttac cgccgcggca
     2341 gtgtcgtaat gtttatgccc gtcaagcgag acggccagcg ccggaaaacc taaatgacgg
     2401 ccttccatcg cggcggctac cgtaccggaa taaataacgt catcccccag attcggcccg
     2461 gcgttaatgc cggacacaac aatgtccggg cgcggacgca tcagagcatt cacgccaaga
     2521 tagacgcaat cggtcggggt tcccatttgc acagcaatat caccattttc aaaggtaaac
     2581 gtgcgcaggg aggattccag tgtcagagaa tttgaagcgc cgctgcggtt acgatcgggg
     2641 gcgaccacct gaacgtcagc aaactcacgc aaggctttcg ccagcgtttg tataccgggt
     2701 gcatgtaccc cgtcatcatt actcagcaat atgcgcataa tcacctgttg tgttgataag
     2761 ttccctgaca acgctggttg caaaactacc cgccggaagc cagaaacgga tctctacggt
     2821 gacgtcatcc caccaattcc agcttaattg ttgcggatac agcaacatcg ctctgcgcgc
     2881 ggcttcaact ttttcgcgca ccagtaaagc ttgtaattca gtttctgcgg cgacagctgc
     2941 cttttcgaat gccagcgctt cacgctgagt tccccattcg ccactacccg gcaatgcggc
     3001 ggttatcatc agctctttat cgttgacgcg acgctgtaat tccgccagtt cttcggtggt
     3061 tgcgacaaac cagctaccac gtccggctaa ttgtagcgca tcgccgtcaa caacttgatt
     3121 aacgtctgct tttttgaggc gctcagcaac aatctgatta aacaacgcac tgcgggctgc
     3181 cgacaaccaa aaactccgtt tattgcgatc gcgcaccgga gtattggttt gcgcccagcg
     3241 ctgcgccccc tgcaagttgc taccgccaat cccaaaacgt tgggcaccga agtagttcgg
     3301 tacacctttt acgcaaatat cgatcagacg ttgttcaacg tcatcgcgat tgctcacttc
     3361 gcgcagaacc agggtaaagg cgttaccttt cagcgcgcct aaacgcagct tgcgcttgtg
     3421 ccgcgcatac tccagcacct ggcagccttc cagttgaaag gcgctcagat cgggcatttc
     3481 cttgcctggc acgcgagcgc ataaccactg ttccgtaaca gcatgtttgt ctttttgccc
     3541 agcgaagctg acttcacggg catgaatttt caggaatttc gccagtgcat ccgccacaaa
     3601 acgggtattg cagccgtttt tgaggattct aaccagaata tgctcacctt caccatcagg
     3661 ctcaaagccc aaatcttcca ccaccacaaa gtcttccgga ttggctttca gcagcccggt
     3721 gccttgcggt ttaccgtgga ggtaagtgag attatcaaac tcaatcattt tgttgcctta
     3781 atgagtagcg ccaccgcttc acaggcaatc ccttccccac gtccggtaaa tccaagtttt
     3841 tccgtagtag tggctttcac gttaacatca tccatatggc agccgagatc ttcggcaata
     3901 aacacgcgca tttgtggaat gtgcggcaac atcttcggtg cctgagcgat aatagtgaca
     3961 tcgacgttgc ccagggtata acccttcgcc tgaatacgac gccaggcttc gcgtagcagc
     4021 tcgcggctgt cggcaccttt aaatgccgga tcggtatccg ggaacagctt gccgatatcc
     4081 cccagcgccg ccgcgccaag caatgcatcg gtcaacgcat ggagcgccac gtcgccatca
     4141 gaatgcgcca gcaatccttt ttcgtaagga atgcgtacgc caccaatgat aattgggcct
     4201 tcaccgccaa aggcatgtac gtcaaaaccg tgtccaattc gcattatgta ttctcctgat
     4261 ggatggttcg ggtgaggtaa aactcggcca gtgccaaatc ttccgggcgc gtgactttaa
     4321 tgttatccgc acggccttcg accaactgag gatggaatcc gcaatattcc agcgccgagg
     4381 cttcgtcggt aatagtcgcg ccttcattta gagcgcgcgt cagacagtca tgtaacagct
     4441 cacgagggaa aaattgcggc gtcagcgcgt gccataagcc gttgcgatca acggtatgag
     4501 caatggcatt tttgcccggt tcggcacgtt tcatcgtatc gcgcactggt gcggctagga
     4561 tccctcccgt gcggctggtt tcgctcaacg ccaacaatcg cgcgaggtca tcctgatgca
     4621 gacaaggacg agcggcgtca tgcaccaata cccactgcgc gtcgccagcg gctttcaaac
     4681 ctgccagcac ggaatcggca cgctcatcac cgccatctac aacggtgatt tgcggatgat
     4741 tcgccagagg aagttgtgca aaacggctat cgccaggact tatggcaatg acgacacgtt
     4801 tcacccgggg atgcgccagc agcgcatgca ccgagtgttc aagaatggtt tgattaccga
     4861 ttgagagata ttgcttagga cattccgttt gcattcgacg gccaaatccg gccgccggaa
     4921 ccacggcgca aacatccaaa tgagtggttg ccatgttaat tcccgggctg atttatcgat
     4981 tgttttgccc cgcagactgt gcgcgcttcg acgcgtcagg caccagacga taaaaagttt
     5041 cgcccggcct ggtcatgctg agttcattac gcgcacgctc ttcgagcgcc tcctggccgc
     5101 cattgagatc gtcaatttcg gcaaaaagtt gatcgtttcg cgctttaagt ttcgcgtttg
     5161 tagcttgctg tgccgccaca tcatcattga cgcgggtata gtcatgtata ccgttcttac
     5221 cgaaccacag cgaatactgt agccagacca gaatagccag caacagcagc gttagtttac
     5281 ccatcctgcc ccctgaaaaa cggcatcatc atcccatgca tccgaagacg actctacatc
     5341 ctctgttggg gataccgcga caacgcgggc aaatgtacca catttgtcca ttgttacgta
     5401 tacccagggc gtgcagaaca taatctcatt attagttacg gtttgaatta tgaacagagg
     5461 agacaagaaa gtacaaatta gcccagtagc cacataaaca gtgcgccaaa cataatgcct
     5521 actgtcacca gggtgaaaac aatactgtag cgtagctttc cgtccatcaa tgaatgcagc
     5581 gcaatcccca ccactaccgc gacgggcatc agcgccagaa agaaaggcca ggtgtagata
     5641 aagaagaaca gcgtgttaga gccataaatc aacatcggca tcgccagcgc aaataaccag
     5701 gagataaaac cgaccacggc accaggcagt gaccatgtgg tttcttcatc ctcagtaagg
     5761 ctgtcgttat ttgttagtgt aatgttatgg ctattacgca tatttgatcc tgttactttg
     5821 acgaaccggg catggaaacc cggtggtgtc tcaggatctg ataatatcgt tctgtctcaa
     5881 cagatctaat aattgctgta ccaaatttgt tactaattgt tcaccattga gatgaatttc
     5941 tgccgattca ggcgcttcgt aaacggaatc tattcccgta aagttgcgca gttcaccggc
     6001 acgcgctttc ttatataagc cttttggatc gcgggcttcg caaatcgcca gcggcgtatc
     6061 gacaaacact tcgataaagc gcccttctcc tacgcgttca cgaaccatct ggcgttcggc
     6121 gcggtgtggc gagataaatg cggtcagcac caccagtccg gcttcaacca tcaaattcgc
     6181 cacttcaccg acgcgacgga tattctcttt acgatcggca tcgctaaaac cgagatcgct
     6241 gcataatccg tggcgaacat tatcgccatc cagcagatac gtactgacgc cgagtttatg
     6301 taacgcctcc tccagcgccc cggcgaccgt tgatttaccg gacccggaga ggccggtaaa
     6361 ccacagcact acaccacgat gaccgtggtg tagctcgcgt tgttgcacag tgaccggatg
     6421 gctatgccag acgacgtttt cgtcatgcag cgccattatt tatcccccag caaatcgcgc
     6481 gcgccccagt gcggaaagtg gcgacgaacc agagcattca attccagttc gaatgcactg
     6541 aattcagatg gcgcagcagt tgcctggcta actggctcgt gcaccatacc ggcacccacg
     6601 gtcacattgc tcaggcgatc gataaaaatc agcccacccg tcaccggatt ttgttgataa
     6661 cgatctaaca ccagcggctc gtcaaaagtg agatccacga ggccgatccc attcagtggc
     6721 aggttttcaa cttcacgctg ggtaaggtta ttaatatcaa cctgatagcg aatgccatca
     6781 acacgcgcgc gcgtcttctt accggcaatt ttgatgtcgt aactctgccc tggagaaagc
     6841 ggctgttccg ccatccatac cacatccacc gacgcgctct gcaccgccgg taacgcttcg
     6901 tctgccgcca gcagcagatc gccacggctg atgtcgatct catccgtcag caccagggtg
     6961 atcgcttctc cggcaaaggc ttcttcgcga tcaccatcaa aagtcacgat ccgcgcgacg
     7021 tttgattcca caccagaggg cagcactttt acacgttgcc cgacttccac gcgaccggat
     7081 gccagcgttc cggcgtagcc acgaaaatcg agattcgggc gattaacgta ctgcaccggg
     7141 aagcgcattg gctgagcatc caccactcgc tggatctcca cggtttccag cacttcgagc
     7201 agtgtcggac cgctgtacca cggcatactt tcactttgcg atgccacgtt gtcgccttcc
     7261 agtgcagaga gcggcacaaa gcggatatcc agattacccg gcagctgccc ggcaaaggtc
     7321 agataatctt cacgaatacg ggtgaacgtc tcttcactgt aatccaccag atccattttg
     7381 ttgatcgcca cgaccagatg tttgatcccc aacagtgtgg agataaaact gtgacgacgg
     7441 gtttgatcga gcacgccttt acgggcatcg atcagtaaga tcgccagttc acatgtcgat
     7501 gcgccagtcg ccatattgcg agtgtactgc tcgtgccctg gggtgtcggc gataataaat
     7561 ttacgcttct cggtagagaa atagcggtag gccacatcaa tagtgatgcc ctgttcgcgc
     7621 tcagcttgca ggccgtccac cagcagagcc agatccagct tttcgccctg ggtaccgtga
     7681 cgcttactgt cgttatgcag cgatgagagc tgatcttcat agatttggcg agtatcgtgc
     7741 agcaggcgac caatcagagt gcttttgccg tcatcgacgc tgccacaggt cagaaaacgc
     7801 agcaggcttt tatgttgttg cgcaatcatc caggcttcga cgccgccttc attggcgatt
     7861 tgttgtgcaa gtgcggtgtt catcttaaaa atacccctga cgttttttca gctccataga
     7921 tcccgcctgg tcgcggtcaa tcacgcggcc ctgacgttca ctggtggtgg aaaccagcat
     7981 ctcttcgatg atttccggca gtgtttgtgc atttgactcc accgcaccgg tcagcggcca
     8041 gcagcccagc gtacggaaac gcaccatccg ttttttaatc acttcgcccg gttgcaggtc
     8101 gatacggttg tcatcaatca tcatcaacat accgtcgcgt tccagaaccg gacgttccgc
     8161 agcgagatac agcggcacaa tgtcgatatt ttccagccag atgtattgcc agatatcctg
     8221 ctcggtccag ttagagagtg ggaagacgcg gatgctttcg cctttgttaa tttgcccgtt
     8281 gtagttgtgc cacagctccg ggcgctgatt tttcggatcc cagcgatgga agcggtcacg
     8341 gaaagagtag attcgctctt tagcgcggga tttctcttcg tcacggcgcg caccaccgaa
     8401 ggcggcatca aaaccgtatt tgttcagcgc ctgtttcagg ccttcagttt tcataatatc
     8461 ggtatgtttc gcgctgccgt gcacgaatgg attaatcccc atcgccacgc cttccgggtt
     8521 tttatgcacc agcagctcgc agccgtaggc tttcgccgta cgatcgcgga actcatacat
     8581 ctcgcggaat ttccagccgg tatcgacatg cagcaacggg aaaggcagcg tacctggata
     8641 aaacgccttg cgcgccaaat gcagcatgac gctggaatct ttaccgatag agtagagcat
     8701 caccggattt gagaattctg ccgccacctc gcgaataatg tggatgcttt ccgcctccag
     8761 ttgccgcagg tgagtaagtc gtatttgatc cataaccgtt cctttgcaat accgctattt
     8821 tcttgccatc agatgtttcg actataggga gcgtaagaga acgaatgaaa ttaccaatta
     8881 gaatgagtag ttccttaacg gaataacgat ttggcaaagc taatatcaaa aagtgcttaa
     8941 ggcaccggat ttctggcgtt taggaagatt tgaaattgtt ttagcgcagc ggaagtttca
     9001 tactatggcg gtaaaaaaat ttgcatggta tttaaggact cactatgttt tccgcattgc
     9061 gccaccgtac cgctgccctg gcgctcggcg tatgctttat tctccccgta cacgcctcgt
     9121 cacctaaacc tggcgatttt gctaatactc aggcacgaca tattgctact ttctttccgg
     9181 gacgcatgac cggaactcct gcagaaatgt tatctgccga ttatattcgc caacagtttc
     9241 agcaaatggg ttatcgcagt gatattcgga catttaatag tcggtatatt tatactgccc
     9301 gcgataatcg taagagctgg cataacgtga cgggaagtac ggtgattgcc gctcatgaag
     9361 gcaaagcgcc gcagcagatc atcattatgg cgcatctgga tacttacgcc ccgctgagcg
     9421 atgctgacgc cgatgccaat ctcggcgggc tgacgttaca aggaatggat gataacgccg
     9481 caggtttagg tgtcatgctg gaactggcag aacgcctgaa aaatacgcct accgagtatg
     9541 gtattcgatt tgtggcgacc agcggcgaag aggaaggaaa attaggcgct gagaatttac
     9601 tcaagcggat gagtgacacc gaaaagaaaa atacgctgct ggtgattaat ctcgataact
     9661 taattgttgg cgataaattg tatttcaaca gcggtgtaaa aacccctgaa gcagtaagga
     9721 aattaacgcg cgacagggcg ctggcaattg cgcgcagtca cggaatagcc gcaacgacca
     9781 atccgggttt gaataaaaat tatccgaaag gcactgggtg ttgtaatgac gcagaaatat
     9841 tcgacaaagc gggcattgct gtactttcgg tggaagcgac taactggaat cttgggaata
     9901 aggatggtta tcagcaacgc gcaaaaacag ccgcattccc tgcgggaaat agctggcatg
     9961 acgtaagact ggataatcaa cagcatattg ataaggcact tcctggaaga atagaacgtc
    10021 gctgccgtga cgttatgcgg ataatgctac ctctggtgaa ggagttggcg aaggcgtctt
    10081 gatgggtttg aaaatgggag ctgggtgttc taccgcaggg gcggggaatt ctaagtgata
    10141 tccatcatcg catccagtgc gcccggttta tccccgctgg cgcggggaac tcttactgct
    10201 tggtatgcgg aatcacaccc tgaacggttt atccccgctg gcgcggggaa cacactaagc
    10261 atacataact gtttttaaac aaatttattc cgcatcaaca atctaccaac taaattaaaa
    10321 catttcctta tttttaaaga acggataact tattgattat caacaggaag aaaagaaacc
    10381 aaacgtaacc catccaaatc caccggaata cgtctgtttt ctccccaggt ctgaaattca
    10441 aaacccgact cgatattggt cgcccaggcc atcaccacat ttccgcaacc agccagttgg
    10501 gtaatttgct gccagatcat ctcccgaata cgttttgatg tatcaccaac atacacaccg
    10561 gcacgcactt ccagtagcca gattgcgagc cgtccacgta agcgcggagg gacattttct
    10621 gtaacaacca cgaccatgct catccgccgc gcccccggtg accactatca cccagcgttt
    10681 caggttcagg gatggcaggc ggtaacatat ccggcgcggg ttgtggtggt tcaatttcac
    10741 ctgcagcaag gacttcctca attaacggta ttaatttgcc cgttaactta gtgctacgga
    10801 aaatatcgcg acaggctaat ctgacttctt tatcaggttc tgcgggttgc ctcgctgcta
    10861 tttcaaatgc ctttggcaca accgaatcaa atttaatgat atcggctatg tcataaacaa
    10921 atgaaagcgg tttgccacta tggataaacc caatagcggg cgcatatccc gcggctaata
    10981 ctgccgcttc agatataccg tacagacatg atgtagcagc actgatgcag cgattcacaa
    11041 catcgccttt ttcccagtct ttaggatcgt atttgcgacc attccatttc acaccatatt
    11101 gtttcgccag taatgcatac gtctggcgaa cgcgagatcc ctcaattcca cgtaactgct
    11161 ccactgaacg gcgagctggc ggtggttcac gaaaacgtaa ttcatacatt ttgcgcacca
    11221 ccttcaaccg tagatcttcc gttaaagcca gctttgccta gtagagtaat ttatctgccc
    11281 gcgcccctcc gggttgtccg gaggagtaaa cgcgaacgcc cgcttcaccg acccagacca
    11341 gcagtgttcc caccgtggcg gccagatgga ctgccgcgtg ggaaactctt gttcccggtt
    11401 cgagcataat gcaggcgacc gatcccactg gaatatgcgt gcggatcccg gttttgtcga
    11461 tcagcacgaa agcgccgtcc agtacgtcga tttgaccgta ctggaggaag atcatggaag
    11521 tgcgatcttt taatgggatc gggctcagcg gtacaaacgt cacgcctctg ctccgggttt
    11581 gatcagcatc agaccacaac cgaacgcccg gcttttgcca tacccctgac tgagacgttg
    11641 cagaaataac ccaggagcgg tgaccgtaag catccccgta taatccacgc tactgaactg
    11701 gatcagttgc cgggagtttt cccgccgcag ttgctgttgt cggtaggcat caaccgaagt
    11761 atccagcagt gtaaaaccgc ttctctcccc ctgcgctgcc agccagtcca gcgcagcctg
    11821 ttgttgatgc aaccagacat cacttccttc cacctgcccc ctcacctgcc gtttcgcctc
    11881 catcagcaag tcgtggcgtt tgcccgcttt acaaattgtt ggattcgccc gcaggttgaa
    11941 acaaagggat tgtccggtac gcaactctgg cgcaaatggt cggcattcga tggtgaaaat
    12001 atcactttcc gccgggcgtt cctgcgacag gacaaaaaag cg
//
LOCUS       DAJVQG010000117        12038 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJVQG010000117 DAJVQG010000000
VERSION     DAJVQG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12038
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.117"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..324
                     /locus_tag="OQM83_002978"
     CDS             <1..324
                     /locus_tag="OQM83_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521185.1"
                     /translation="AASMLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLI
                     AIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATP
                     ASNVD"
     gene            458..1535
                     /gene="ygjJ"
                     /locus_tag="OQM83_002979"
                     /pseudo
     CDS             458..1535
                     /gene="ygjJ"
                     /locus_tag="OQM83_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            1552..3903
                     /gene="ygjK"
                     /locus_tag="OQM83_002980"
     CDS             1552..3903
                     /gene="ygjK"
                     /locus_tag="OQM83_002980"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCS1521186.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWLPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            4129..6147
                     /gene="fadH"
                     /locus_tag="OQM83_002981"
     CDS             4129..6147
                     /gene="fadH"
                     /locus_tag="OQM83_002981"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCS1521187.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(6192..6608)
                     /gene="higA"
                     /locus_tag="OQM83_002982"
     CDS             complement(6192..6608)
                     /gene="higA"
                     /locus_tag="OQM83_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCS1521188.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(6605..6919)
                     /gene="higB"
                     /locus_tag="OQM83_002983"
     CDS             complement(6605..6919)
                     /gene="higB"
                     /locus_tag="OQM83_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCS1521189.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(7204..8340)
                     /gene="rlmG"
                     /locus_tag="OQM83_002984"
     CDS             complement(7204..8340)
                     /gene="rlmG"
                     /locus_tag="OQM83_002984"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCS1521190.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            8425..8928
                     /locus_tag="OQM83_002985"
     CDS             8425..8928
                     /locus_tag="OQM83_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCS1521191.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            9005..9688
                     /locus_tag="OQM83_002986"
     CDS             9005..9688
                     /locus_tag="OQM83_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCS1521192.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            9767..10753
                     /locus_tag="OQM83_002987"
     CDS             9767..10753
                     /locus_tag="OQM83_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1521193.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            11036..12001
                     /gene="alx"
                     /locus_tag="OQM83_002988"
     CDS             11036..12001
                     /gene="alx"
                     /locus_tag="OQM83_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCS1521194.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         2862 a         3094 c         3226 g         2856 t
ORIGIN      
        1 gcagcgtcca tgcttccgcc gttattcatc atgctggctt acctgaattt acgcgccaaa
       61 ttagatcacc tgccacgcga tttccgtatg ggctcccgcc gcaccggtat tatcgttgtt
      121 tcaatgctga ttgcgatatt tgccgtaggg tttgtcgctt cgacattccc gactggcgcg
      181 aatattctga ccatcatttt ttataacgtc ggcggtattg ttatcttcct tggctttgcg
      241 tggtggaaat acagtaaata tataaaggga ttaacggctg aagagcgcca tattgaagcg
      301 acgccagcca gcaatgttga ttaagcagaa taataaaaaa tgagcaggca ttttccctct
      361 tcctggatga ggagagggaa aatagtttct gcctttatat ttttataatt acaacgataa
      421 aaggctgtac tttttcttta gctcatggat taacacaatg aaattaatca ctgcaccatg
      481 cagagcatta cttgctctgc cgttttgcta cgccttttct gcggcaggag aagaagcacg
      541 tccggcagaa catgacgaca caaaaacacc cgcaattacc tcgacatctt ctccttcatt
      601 tcgtttttac ggcgaattag ggattagggg gtggtggata tatggattta gagggtgaga
      661 ataaacataa atacagcgac ggtacctata ttgaaggtgg cctggagatg aagtacggct
      721 cctggttcgg cctgatttac ggcgaaggct ggaccgtgca ggccgaccac gacggcaatg
      781 cctgggtgcc agaccatagc tggggtggtt tcgagggcgg aattaaccgt ttctatggcg
      841 gttatcgtac caatgatggc accgaaatca tgctcagtct gcgtcaggat tcctcgctgg
      901 atgacctgca atggtggggc gatttcaccc ccgatctggg ctacgtcatt cccaataccc
      961 gcgacattat gactgcgctg aaggtacaga acttaagcgg caactttcgt tatagcgtca
     1021 ccgcgactcc tgccggacat catgatgaaa gcaaagcctg gctacatttt ggcaaatacg
     1081 atcgctatga cgacaaatac acctatccgg caatgatgaa cggttacatc cagtatgacc
     1141 ttgccgaagg catcacctgg atgaacggtc tggaaatcac cgacggcaca ggacagctct
     1201 atctcacggg cctgctaact cctaactttg ccgctcgcgc ctggcaccat accggacgcg
     1261 ccgacgggct ggacgtaccg ggaagtgaaa gtgggatgat ggtgagcgcc atgtatgaag
     1321 cgttaaaggg cgtttatctc tccaccgctt acacctacgc caaacatcgc cctgaccacg
     1381 ctgacgatga aaccacctct ttcatgcagt ttggtatctg gtacgaatac ggcggcggac
     1441 gtttcgccac ggcttttgat agccgcttct acatgaaaaa tgcctctcac gatcccagcg
     1501 accaaatctt cctgatgcaa tatttctact ggtaataagg actgtaattt catgaaaata
     1561 aaaactattt taacgccagt aacctgcgct ctgctgataa gtttttccgc ccatgccgct
     1621 aacgccgaca attataaaaa cgtgattaac cgtactggcg cgccgcagta catgaaggat
     1681 tacgattacg acgatcacca gcgttttaat ccgtttttcg atctcggagc ctggcatggt
     1741 catctgttgc cagacggccc taacaccatg ggcggctttc cgggcgttgc gctgctgacg
     1801 gaagagtaca tcaactttat ggccagcaat ttcgaccgcc tgaccgtctg gcaggacggc
     1861 aagaaagtcg actttacgct ggaggcatac agtattcccg gtgcgctggt gcaaaaactg
     1921 acagcaaaag atgtgcaagt cgaaatgact ctgcgcttcg ccacgccgcg cacgtcacta
     1981 ctggaaacca aaatcaccag caataaaccg ctggatctgg tgtgggacgg cgaactgctg
     2041 gaaaaactgg aagcgaaaga agggaaaccg ctttccgata aaaccattgc tggcgaatac
     2101 cctgactatc agcgcaaaat cagcgccacc cgtgatggcc tgaaagtcac ctttggcaaa
     2161 gtgcgcgcca cctgggatct gctgacctcc ggcgaatcgg aatatcaggt gcataaatcc
     2221 ctgccggtgc agactgaaat caacggtaat cgctttacca gtaaggcgca tatcaacggt
     2281 tcgaccacgc tctacaccac ctattcccat ctgctgaccg ctcaggaagt tagcaaagag
     2341 caaatgcaga tccgcgatat tctggcgcgt ccggcgtttt atctcaccgc ctcgcagcaa
     2401 cgctgggaag aatacctgaa gaaagggtta accaatccgg atgcgacgcc ggaacagacg
     2461 cgcgtcgcgg tgaaagcgat cgaaacgctc aacggtaact ggctctcacc gggcggtgcg
     2521 gtgaaattta acaccgttac accgtcggtg accgggcgct ggttctccgg caatcagacc
     2581 tggccgtggg atacctggaa gcaggcgttt gcgatggcgc atttcaatcc ggacatcgcc
     2641 aaagagaata tccgcgcggt cttctcctgg cagatccagc ctggcgatag cgtgcgtccg
     2701 caggatgtgg gctttgtccc cgacctgata gcgtggaacc ttagccccga gcgtggtggc
     2761 gatggcggca actggaacga acgtaatacc aaacccagcc ttgccgcctg gtcggtgatg
     2821 gaagtgtaca acgtcaccca ggataaagcc tggttggcag agatgtaccc gaaactggtg
     2881 gcctatcacg actggtggtt acgtaaccgc gatcacaacg gcaacggcgt gccggaatat
     2941 ggcgcgaccc gcgacaaagc ccacaacact gagagcggcg agatgctgtt tacggtgaaa
     3001 aaaggcgata aagaagagac gcagtctggg ctgaacaact acgcccgcgt ggtggagaaa
     3061 ggcctgtacg acagtctgga aattccggca caggttgctg catcgtggga atcggggcgt
     3121 gatgatgccg ccgtctttgg gtttatcgac aaagaacagc tggataaata tgtcgctaat
     3181 ggcggcaaac gtagcgactg gacggtgaaa ttcgccgaaa accgcagtca ggacggaacg
     3241 ttgctgggct actcgctatt gcaggagtcg gtggatcagg ccagctatat gtacagcgat
     3301 aaccattatc tggcggagat ggcaaccatc ctcggtaagc cagaagaggc caaacgctat
     3361 cgccagttgg cacagcagct cgcggactac atcaacacct gtatgttcga cccgactacc
     3421 cagttctact atgacgtacg tattgaagat aaaccactgg cgaacggctg cgcgggcaaa
     3481 ccgattgttg agcgcggtaa agggccggaa ggctggttgc cgctgtttaa cggtgcggca
     3541 acgcaggcca acgccgacgc ggtggtgaag gtgatgctcg atcctaaaga gttcaacacc
     3601 tttgtcccgc tgggaacggc ggcgttaacc aatccggctt ttggcgctga tatctactgg
     3661 cgtgggcgcg tatgggtgga tcagttctgg tttggtctga aagggatgga gcgttacggt
     3721 tatcgtgatg atgccctgaa gctggcggat acgttcttcc ggcacgccaa aggattaacc
     3781 gccgatggcc caattcagga gaattacaac ccgctgactg gcgcacagca aggcgcacca
     3841 aatttctcct ggagtgccgc tcatttgtat atgttgtata acgatttttt ccgtaagcag
     3901 taatttgcaa cggctggcgg atgcggcgtg aacgccttat ccgccctaca tgtgcaatcc
     3961 cccaggccgg ataaaacgcg gcaagcgttg catccggcgt taccaccggg cgtattcttt
     4021 ttgaatccca tcacaaaccc cgcactcccc ttttcccttt tctccatcga cggctaaatt
     4081 agaactcatc cgaccacata acaattattt tacatactgg acaattttat gagctatccg
     4141 tcgctgttcg ccccgctgga tttaggtttt accacgttaa aaaaccgcgt gttgatgggc
     4201 tcaatgcaca ccgggctgga ggaatacccg gacggtgccg agcggctggc agcgttttat
     4261 gccgaacgcg cccgtcacgg cgtggcgctg attgtcagcg gcggcattgc accagattta
     4321 acaggcgttg gcatggaagg tggcgcaatg ctcaacgacg ccagccagat cccacaccat
     4381 cgcaccatta ccgaagcggt acatcaggaa ggcggcaaaa tagccctgca aattttgcat
     4441 accgggcgct acagctacca accgcatctg gtcgctccct ccgcattgca ggcccccatc
     4501 aaccgtttcg ttcctcacga actcacccat gaagagatcc tgcaactgat cgacgatttc
     4561 gcccactgcg cgcaactggc acgggaggca ggatacgacg gtgtagaggt aatggggtcc
     4621 gaagggtatt tgatcaacga atttctgacg ctgcgcacca atcagcgtag tgaccagtgg
     4681 ggcggcgatt accgcaaccg gatgcgattt gccgtagaag tagtgcgtgc ggtgcgcgaa
     4741 cgcgtcggca acgacttcat tattatctac cgactgtcga tgctcgacct ggtcgaagac
     4801 ggcgggactt ttgccgaaac ggtagagctg gcgcaggcca ttgaagcggc gggcgcgacc
     4861 attatcaaca ccggcattgg ctggcatgaa gcacgtattc cgaccattgc cacgcccgtg
     4921 ccgcgcggcg catttagctg ggtcacgcgc aaattgaaag gccacgtctc gctgccgctg
     4981 gtaaccacca accggattaa cgatccgcag gttgccgacg atattctctc gcgcggcgat
     5041 gccgatatgg tatcgatggc acgaccgttt cttgctgatg cggaactgct gtcaaaagcg
     5101 caatcggggc gagccgatga gatcaacacc tgcattggct gcaatcaggc ctgcctcgat
     5161 cagatcttcg ttggcaaagt cacctcgtgc ctggtgaatc ctcgcgcctg ccacgaaacc
     5221 aaaatgccaa tccttcctgc cgtgcagaaa aaaaatctgg cggtggtcgg tgcgggacct
     5281 gctgggctgg cgtttgccat taacgcggcg gcgcgtgggc atcaggtaac attgtttgat
     5341 gcccacagcg agatcggcgg gcagtttaat atcgccaaac agatccccgg caaagaggag
     5401 ttttacgaaa cgctgcgcta ttaccaccgg atgatcgaag tgacgggcgt gacgctgaaa
     5461 ctcaatcaca ccgtgacggc ggatcagtta caggcgttcg atgaaacgat cctcgccagt
     5521 gggatcgtgc cgcgcgttcc gcctatcgac gggatcgatc atccgaaggt attgagttat
     5581 ctcgatgtac tgcgcgacaa agcgccggta ggcaacaagg ttgccatcat cggttgtggc
     5641 gggattggtt ttgatacggc gatgtattta agtcagccgg gcgaatccac cagccagaat
     5701 atcgccaggt tctgtaatga gtgggggatc gacagcagcc tacaacaggc tggtggctta
     5761 agcccgcagg gaatgcagat cccgcgtagc ccacggcaaa ttgtgatgct ccagcgcaaa
     5821 gccagcaaac cgggacaggg gttaggcaaa accaccggct ggatccatcg caccaccctg
     5881 ctctcgcggg gtgtgaaaat gatcccaggc gtaagttatc agaagattga cgatgacggg
     5941 ctgcatgtgg tgatcaacgg cgaaacgcag gtattagcag tggacaatgt ggtgatctgc
     6001 gcagggcaag agccaaaccg cgcgctggcg caaccgctga ttgatagcgg gaaaactgtg
     6061 catttaattg gcggctgcga tgtggctatg gagctggacg cacgacgggc aattgcccag
     6121 ggaacacggc tggcgctgga gatttaaatc gttattttaa gcgccggatg caacgcatcc
     6181 ggcacgtcgc gttaatcaat aaacaaggcg ggagaaatgc cgaatcgcgt tgccaatttt
     6241 ttagcgtgtt ccagcgttaa tttcctcttc ccgctcaaaa cgcgtgacac catagattta
     6301 ctgccaattt ccggcagatc ggcaagggtt aaaccatatt gatccataag ggtacgaatc
     6361 acggctatac cgccaggcat ggcttgagcc atggcattaa attccgcaaa ttcgggcgct
     6421 gattcttccc acgcggttat tttggcacac accagatcca gcaaggggtt ttcaggatcg
     6481 ttgagcagca gatgatctac cagttccagc gcctgggtgt attgttcctc gttctgaata
     6541 cccgccagaa aaggtgccac cgcagttagc ttttctcctg cttgcaagat gtcggcaatc
     6601 gcaatcattt tttcccctta gtacgatgaa cagcggtaaa gaaatcgtat tctttatgcg
     6661 tcataacttc acgtatgtag cacttttgcg attcaaaaaa gaccattgct acaacacgta
     6721 attcattgcc cccaacattg aaaacataat gcttatccag atatttgaag ttatccagag
     6781 atgggaatac tgcttttaat gactcaggtt ttttgaaata tcccttagca atcgtgttcc
     6841 ccagagccac caactccgtt ttatgttgcg ggtatttttc cgcagcatct ttcaatgctt
     6901 tttgagttat caggtgcatt cttcatcacg tccgttgaca aattggcaat atgataacat
     6961 ccgttgacag attggcaacg gatgaattta tctgtgtgac ggaagaataa acgtagaaaa
     7021 acgctaaaca agagaatgta gaggatggtg agagggattt tttgttttat ggaagcgcga
     7081 ttccagaact gggcagacat tgccggatgc gacgcttgcc gcgtcttatc cggcctggga
     7141 gtaacgtgct gaacgtaggc cggataaggc gttaacgccg catccggcag gtaactccca
     7201 atcttaccga cgacgcccca gcttcactgc tttcagcacc acaaatttat tattcgtggc
     7261 gatggtggtg cagttgccga aaatcttctt cagtttatgg aagtaatcca ggtgacggtt
     7321 ggcaacaata tacagctcgc cgttgatttt caggcagcgg cgggcatggt ggaacatctc
     7381 ccaggcgacg ttatcggtca gcgcatgttg ttggtgaaac ggcgggttgc agagcacagc
     7441 attaaagcgg aaaggctcca cgccggagag cgcgttgttg atcataaact cgctgcgatc
     7501 taacgcctct ggcatgttgg tttcaacgtt caaacggctg gaagcaaccg ccatcggcga
     7561 ttcatcgaca aacaccactt tcgcctgagg gtttttatca agcagcgtca gaccaataac
     7621 gccattaccg caaccgagat cgacaatctc accttcgaga ttctctggca gatgttgcat
     7681 aaagaagcgt gcgccaatat ccagcccggt gcgggagaag acattcgcat ggttgtggat
     7741 agtccagtca gtaccttcca gcttccagct aacggtctgc ggcgcatcgg ccagcggcgg
     7801 ttcattgaaa gtgcaattaa tcaggcgcgc ttttttccat gccagcgtag tggtggtcgg
     7861 gccgagcact ttctcgaaca gctccagcgt ggaagtgtga atgtcacggg ctttggcacc
     7921 ggcaataata cgtgtatccg acgtgaccac tttgcgcagc gcacgcagtt gctgttccag
     7981 caatgccagt gttttcggca ctttgatcag caccacaccc ggctgttgcg ggtagtcggc
     8041 ggtgctgtcg agaaacttca cgctcgactc atcaatcccg ttgaggcgta aattctcgcg
     8101 cgtcgccagt tcactgatgt atgagtcgcc aatgctgtac ggcttatgtt ccgccagcgc
     8161 gcagcttaac gcaccaaagg catcattcag gatcaacacc gggccgcgga tttctgtgtc
     8221 gtccaactgt tgcagcaaat attcatccgc cgcttcccac gcctgtagcg ggttaacgtc
     8281 atccgtcgcc ggaaaacgtt gtagtgtcag tgaacggaaa ccgttgtcta agtggctcat
     8341 cggccctcct gaatgataaa atttcgacgt tgcccctgaa aagggtgcgt gagtataccc
     8401 tttttctctt tttcgtgggc ggttatgagc aatcttactt atcttcaggg ttatcccgag
     8461 cagctacttt cccaggtgcg aacgctgatt aacgaacagc gtctgggcga tgtgctggca
     8521 aaacgctatc cgggaacaca cgactacgcc accgataaag ccctctggca atatacccag
     8581 gatctaaaaa atcagtttct gcgcaatgcc ccgccgatca ctaaagtgat gtatgacaat
     8641 aagatccacg tactgaaaaa cgcgctcgga ttacataccg ctgtttctcg tgtgcagggc
     8701 ggcaagctga aagcaaaagc agagatccgc gtcgctactg tgtttcgcaa cgcgccggaa
     8761 ccgtttttac gcatgatcgt ggtgcacgaa ctggcgcacc tgaaggagaa agagcataac
     8821 aaagcgtttt accagttgtg ctgccatatg gagccgcagt accaccagct tgagttcgac
     8881 acccggctgt ggctaacgca gttgtcgctt gggcaggaca aaatctgaaa atgatgtcag
     8941 gcgttatctt ttctttcttt agcgcctaaa atcgacctcc cccctttcgt taaggataac
     9001 gaccatgcag ccccgccttc ttctccgtat ttgtttttct cgccggactc taaagattac
     9061 ctgcttactg ttacttgtgg caggagcgac catttttatc gccgatcgcg taatggtcaa
     9121 cgccagcaag cagctgacct ggagcgatgt caacgcagtt ccggcgcgta acgtgggttt
     9181 attgctgggg gcgaggccgg gtaatcgcta ctttacccga cgtattgata ccgcagcagc
     9241 gttgtaccac gtcggaaaag tgaaatggct gttagtcagt ggcgataacg gacgtaaaaa
     9301 ttacgatgaa gcctcaggta tgcagcaggc tttgatcgct aaaggcgtac cggcgaaagt
     9361 gatcttctgc gactatgccg gattctcaac gcttgattca gtggtgcgtg ccaaaaaggt
     9421 gtttggggaa aaccatatca ccattatctc gcaagaattt cataaccaac gcgccatctg
     9481 gctggcaaaa cagtacggca tcgacgctat cgggtttaac gcgccagacc ttaatatgaa
     9541 gcacggtttt tatactcaac tgcgggagaa actcgctcgt gtcagcgcgg tgattgacgc
     9601 caaaatcctc catcgccagc cgaaatatct cgggccatcg gtcatgattg ggccgtttag
     9661 tgagcatggc tgtccggcga aagaataatg cgtatctgcg cacgtcgaag atgaaaaagg
     9721 cgtgctacat tgacgacaga atccctttat ggagtatcca cgcgttatga tacgtttcgc
     9781 tgtgattggt acgaactgga tcactcgcca gttcgtcgag gccgcccatg agagcggtaa
     9841 atacaagtta accgccgtat attcccgcag ccttgaacag gcccagcact tcgccaatga
     9901 tttttctgtc gagcatctgt ttacctcgct ggaagcgatg gcggaaagcg atgccattga
     9961 cgcggtgtat attgccagcc cgaatgccct gcacttttcc cagacacaac ttttccttag
    10021 ccataaaatt catgtgattt gcgagaaacc actggcgtcg aatctggcgg aagtggatgc
    10081 cgccattgcc tgtgcgcggg aaaatcaggt ggtgctgttt gaggcattta aaaccgccag
    10141 cctgccgaac tttcatttgc tgcgccaggc gctgccgaaa gtcggcaaat tgcgtaaagt
    10201 ctttttcaac tattgccagt attcctcgcg gtatcaacgt tacctggatg gtgagaatcc
    10261 caacaccttt aatccggcat tctctaacgg ttcaattatg gatatcggct tttactgcct
    10321 ggcgtcggcg gtggcattat tcggtgaacc gaaaagcgtg caggcaaccg ccagtttgtt
    10381 ggcaagcggc gtagatgccc acggcgtggt ggtgatggat tacggtgatt tcagcgttac
    10441 gttgcagcac tcaaaggtca gtgattctgt cctggaaagc gagattcagg gcgaagcggg
    10501 atcgctggtg attgaaaaac tgtctgaatg ccagaaagtg tgcttcgtgc cgcgtggcag
    10561 ccagatgcag gatctcaccc agccgcagca tattaatacc atgctctacg aagcagagct
    10621 gtttgctacc ctggtggatg agcatctggt ggatcatcca gggctggcgg tcagtcgcat
    10681 caccgccaaa ctgctgactg agatccgccg ccagactggg gtgatttttc cggcagatag
    10741 cgtaaaacta taattgccaa agtaaaacag tgtaaaaggt atgtaacaga ccattgactg
    10801 gctgaatggt ctgtcatact ttgttacctg caaaggggag taacttcatt gccggtcgat
    10861 cgtcattacg atgtgtgaaa aaccacatcc ggtcaccggg caacccgaaa ggaatacgca
    10921 gacgtattcc ttttttgttg taagtgagac cttgccggaa ggcgaggtct atgcataaaa
    10981 agcagcggct gacgtcttcc gacgttggcc gtttttttat gtgtaaggaa cttctatgaa
    11041 tactgtcggc acgccgttgc tatggggcgg attcgctgtt gttgtcgcca ttatgctggc
    11101 tatcgacctg ttgttgcagg ggcgtcgtgg ggcacatgcc atgaccatga aacaggctgc
    11161 ggcctggtcg ctggtctggg tgacgctgtc gttactgttt aacgccgctt tctggtggta
    11221 tctggtgcaa actgaaggtc gcgccgttgc cgatccacag gcactggcct ttctcacggg
    11281 ttatctgatt gagaaatcgc tggcggtcga taacgtcttt gtctggctga tgttgttcag
    11341 ctatttctct gttccggcgg cattacaacg ccgcgtgctg gtgtatggcg tgctcggggc
    11401 gattgttctg cgtaccatca tgatcttcac tggcagctgg ctaatttcgc agttcgactg
    11461 gatcctgtat atcttcggtg ccttcctgct gtttaccggc gtgaagatgg cgctcgccca
    11521 tgaagatgaa tcaggcattg gcgacaagcc gctggtgcgc tggctacgcg gtcatttgcg
    11581 catgaccgac accatcgaca acgagcattt ctttgtgcgt aagaatggat tgctgtacgc
    11641 cacaccgctg atgctggtac tgattctggt ggaattgagc gacgtgattt tcgccgtgga
    11701 tagcattccg gctatcttcg ccgtgaccac tgacccgttc attgtgctga cctcaaacct
    11761 gtttgcgatc ctcggcctgc gtgcgatgta tttcctgctg gcgggcgtag cagagcgttt
    11821 ctcgatgctc aaatatggcc tggcggtgat tctggtgttt atcggtatca agatgctgat
    11881 tgtcgacttc taccatattc caatcgccgt ctcgctgggc gtggtgtttg gcattctggt
    11941 gatgacgttt attatcaacg cctgggtgaa ttatcggcat gataagcagc ggggagaatg
    12001 attcgctgta tattaagcca ttcctagaga taactcat
//
LOCUS       DAJVQG010000118        11960 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJVQG010000118 DAJVQG010000000
VERSION     DAJVQG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11960
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.118"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..1760
                     /locus_tag="OQM83_002989"
     CDS             <1..1760
                     /locus_tag="OQM83_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HCS1521195.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYE
                     RDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGN
                     LFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWL
                     NKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVI
                     INKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIP
                     GRSALLLGKTGEPPNYLYL"
     gene            2096..3073
                     /gene="csiD"
                     /locus_tag="OQM83_002990"
     CDS             2096..3073
                     /gene="csiD"
                     /locus_tag="OQM83_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCS1521196.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3093..4361
                     /gene="lhgO"
                     /locus_tag="OQM83_002991"
     CDS             3093..4361
                     /gene="lhgO"
                     /locus_tag="OQM83_002991"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCS1521197.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSKHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4384..5832
                     /gene="gabD"
                     /locus_tag="OQM83_002992"
     CDS             4384..5832
                     /gene="gabD"
                     /locus_tag="OQM83_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HCS1521198.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            5846..7126
                     /gene="gabT"
                     /locus_tag="OQM83_002993"
     CDS             5846..7126
                     /gene="gabT"
                     /locus_tag="OQM83_002993"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HCS1521199.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7437..8837
                     /gene="gabP"
                     /locus_tag="OQM83_002994"
     CDS             7437..8837
                     /gene="gabP"
                     /locus_tag="OQM83_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCS1521200.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            8858..9520
                     /gene="csiR"
                     /locus_tag="OQM83_002995"
     CDS             8858..9520
                     /gene="csiR"
                     /locus_tag="OQM83_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCS1521201.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9521..9970)
                     /gene="kbp"
                     /locus_tag="OQM83_002996"
     CDS             complement(9521..9970)
                     /gene="kbp"
                     /locus_tag="OQM83_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCS1521202.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10054..10212)
                     /locus_tag="OQM83_002997"
     CDS             complement(10054..10212)
                     /locus_tag="OQM83_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCS1521203.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10395..10694
                     /locus_tag="OQM83_002998"
     CDS             10395..10694
                     /locus_tag="OQM83_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1521204.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10704..11228
                     /gene="ygaP"
                     /locus_tag="OQM83_002999"
     CDS             10704..11228
                     /gene="ygaP"
                     /locus_tag="OQM83_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCS1521205.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(11275..11679)
                     /gene="stpA"
                     /locus_tag="OQM83_003000"
     CDS             complement(11275..11679)
                     /gene="stpA"
                     /locus_tag="OQM83_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCS1521206.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2985 a         2947 c         3078 g         2950 t
ORIGIN      
        1 atgtatataa tattaacgtt acgtcacctt ccggacagca agaaacattt aaaatactaa
       61 tcaatccaga acatctacgg cagacacttg agcgtaaatc tcttactgct gttcagaaat
      121 cacaatgtga aatcatcacc cctaaaaaac ccggcgaagc gattcttcat gcttttaatg
      181 ccacctacca gcaaatcagg gaaaatatgt ctgagtttgc acgttgccat tatgggtata
      241 tacaaatccc tccagtgaca actttccgcg ccgacggacc agaaactccc gaagaagaaa
      301 agggttactg gtttcatgct tatcaacccg aagatctttg taccatccat aatccaatgg
      361 gagatttgca ggattttatt gcattggtta aagatgctaa aaaatttggt atcgatatca
      421 ttcctgatta tacctttaac tttatgggaa ttgggggtag tggtaaaaat gacctggatt
      481 atccctctgc tgatatacga gcgaagatca gtaaagatat agaaggtggt atccctggct
      541 attggcaagg tcaggttttg attccattca ttaaagatcc agtaacaaaa gaacgtaaac
      601 aaatccatcc agaagatata catctcactg caaaagactt cgaagcaagt aaagataaca
      661 tctctaagga tgaatgggaa aacctccatg cattaaaaga aaagcgttta aatggaattc
      721 ctaaaacaac acccaaaagt gaccaggtta ttatgttgca aaatcaatac gttcgtgaaa
      781 tgcgaaaata tggcgtacga ggtttacgtt atgatgcggc aaaacactca aaacatgaac
      841 aaatagaaag atcaataacc ccaccgctta aaaattataa tgagcggtta cacaatacta
      901 acttatttaa cccaaaatat cataaaaaag ccgttatgaa ttacatggaa tatctggtaa
      961 cttgtcagtt ggatgaacaa caaatgtcat cactgcttta tgaaagagat gatttaagcg
     1021 ccattgattt ttcattgctc atgaagacga taaaagcctt ttcattcggg ggagatctcc
     1081 taacccttgc atcaaaaccg ggttccacaa tctcaagcat cccatcagaa agacggatat
     1141 tgattaacat taaccacgat tttcctaaca atggcaatct tttcaatgac tttctattta
     1201 accatcaaca agatgaacaa ttagcaatgg catatatggc cgctctcccg ttcagcagac
     1261 ctttagttta ctgggatggc caggtattaa aatcaacgac tgaaattaaa aattatgatg
     1321 ggtccacgcg tgtcggtggt gaggcgtggc ttaataaagg ttgctctacc tatcagcagc
     1381 tctacaatga attccacgca ttatatatag ataaagcagg aatatggagc gcatttgagg
     1441 gtgtatttgc aactaaaaac gttctagcct ttagtcgtgg ggattctgtg aacattaatc
     1501 actctcctca tgatggacta gttataataa ataaaggaaa cgaagaagtt gaaggtacct
     1561 ggcctaacaa attgcaacct ggaatatata aaaacatggg gagtaatagc gttaacatta
     1621 ttattaataa tacccgaaaa attatccccc ctggtaaagt atttacgctt agaggcggaa
     1681 ctctaaatat caatattcct gggcgtagcg ctcttctttt agggaaaaca ggagaaccgc
     1741 cgaactatct ctatttataa tttaatgtta tatctgcccc gataaaacgg ggcagataat
     1801 atgtttagtt tactaacggt cattttgcag tgaagccatt tactgttttt tatcgaccag
     1861 ataatctgtt ctctaatgtt aactccccct aacctgttgc tttagttatt catttcctgt
     1921 ctcactttgc cttaatactc tacgttaaat gttactaatt tgttgctttt gatcacaata
     1981 agaaaacaat atgtcgcttt tgtgcgcatt tttcagaaat gtagatattt ttagattatg
     2041 gctacgaaat gagcatcgcc atgtcaccct acatctcata agaggatcgc ttctgatgaa
     2101 tgcactgacc gccgtacaaa ataacgctgt cgattcaggc caggactata gcggattcac
     2161 tctcatcccg tcggcgcaat ccccgcgtct gctggaactc accttcaccg aacagacgac
     2221 caaacagttt ctcgagcagg ttgccgaatg gcccgtacag gcgctggagt acaaatcgtt
     2281 tctgcgtttt cgggtaggca aaattcttga cgatctgtgt gcgaatcagc tgcaaccatt
     2341 gctgttgaag accctgctaa accgtgctga aggtgcgctg ttgatcaatg cggtgggtat
     2401 cgatgatgtg gcgcaggcag atgagatggt gaagctggca acggcggtag cgcatctgat
     2461 tggtcgctca aatttcgatg cgatgagcgg tcagtattac gcgcgtttcg tggtgaaaaa
     2521 tgtcgataac tcagacagct atctgcgtca gccgcaccgc gtgatggaac tgcacaacga
     2581 cggcacgtat gtcgaagaga tcaccgatta cgtgctgatg atgaaaatcg atgagcaaaa
     2641 catgcagggt ggaaattcgt tgctgctgca tctcgatgac tgggaacatc tggaccacta
     2701 tttccgccat ccactggcgc gtcgcccgat gcgctttgcc gcgccgccga gcaaaaacgt
     2761 cagcaaagat gttttccatc cagtgttcga cgttgatcaa cagggtcgcc cggtgatgcg
     2821 ctatatcgac cagttcgtcc agccaaaaga ttttgaagaa ggcgtgtggt tgagcgagct
     2881 ttccgatgcc attgaaacca gcaaaggcat tctttctgta cccgttcccg ttggcaaatt
     2941 cctgttgata aacaacctgt tctggctgca cggtcgcgac cgctttactc cgcacccgga
     3001 tctgcgccgt gaactgatgc gtcagcgtgg ctatttcgct tacgccactc accactacca
     3061 gacgcatcag taagcgcaaa ggaattgagc ggatgtatga ttttgtgatt attggcggcg
     3121 gcatcatcgg catgtcgacc gccatgcaac tgattgatgt ttatccggat gcccgcattg
     3181 cgttgctgga aaaagagtcc ggcccggcct gtcaccagac gggccacaac agcggcgtga
     3241 tccatgccgg ggtctattac acgcccggca gcctgaaggc gcagttttgc ctggcgggga
     3301 accgcgccac caaagccttt tgcgatcaaa acggcattcg ctatgacaac tgcggcaaga
     3361 tgctggtcgc cacgtctcag ctcgaaatgg aacggatgcg cgccttatgg gaacgcacag
     3421 cggcgaacgg tatcgagcgc gagtggataa acgccgtgga actgcgcgag cgcgaaccga
     3481 atatcaccgg gcttggtggc atttttgtgc cgtccagcgg cattgtcagc taccgcgaag
     3541 tcacagcggc aatggcaaaa atcttccagg ccagaggcgg cgagattatt tataacgccg
     3601 aagtcagcgc cctcagtaag cataaaaacg gcgtggtgat acgcacccgt cagggcggtg
     3661 aatatgaagc atcgacgctg attagctgtt ccgggctgat ggctgaccgg ctggtgaaaa
     3721 tgctcggcct cgaaccgggc tttatcatct gcccgttccg tggtgagtat ttccgccttg
     3781 cgccggagca taaccagatt gttaaccacc tgatttaccc cattcccgac cccgcgatgc
     3841 catttttggg cgtgcatctc acccgcatga tcgacggcag cgtgaccgtc gggccaaacg
     3901 cggtgctggc tttcaaacgc gaaggctatc gcaagcgcga cttctcattt agtgacacgc
     3961 tggagatttt gagctcgtcg gggattcgcc gggtgctgca aaaccatcta cgctcaggac
     4021 tgggcgagat gaaaaactcg ctgtgcaaaa gcggctacct gcggctggtg caaaagtatt
     4081 gtccccggct ttcgttaagc gatctccagc cctggcccgc cggtgtgcgg gcgcaggcag
     4141 tatcgccgga cggcaagctg attgacgatt ttctgtttgt caccacccca cgcacgatcc
     4201 acacctgcaa tgcgccctcc ccggcagcga catcagcaat tcctattggt gcgcatattg
     4261 tcagcaaggt acaaacgctg ttggcaagcc agagtaaccc cggacgcacg ctgcgagcgg
     4321 cacgtagtgt ggatgcctta cacgccgcat ttaatcaata acctttgaaa acaggacgta
     4381 acaatgaaac ttaacgacag taacttattc cgccagcagg cgttgattaa cggggaatgg
     4441 ctggacgcca acaatggcgg gatcatcgac gtcaccaatc cggcgaacgg cgacaagctg
     4501 ggtagcgtgc cgaaaatggg cgctgatgaa acccgcgccg ctatcgacgc cgccaaccgc
     4561 gccctgcccg cctggcgtgc gctcaccgcc aaagaacgcg ccaacattct gcgcaactgg
     4621 ttcaatttga tgatggagca tcaggacgat ttagcgcgcc tgatgaccct cgaacagggc
     4681 aaaccactgg ccgaagcgaa aggcgaaatc agctacgctg cctcttttat tgagtggttt
     4741 gccgaagaag gcaaacgtat ttatggcgat accattcccg gtcatcaggc cgataagcgc
     4801 ctgattgtta tcaagcagcc gattggcgtt accgcagcta tcacgccgtg gaacttcccg
     4861 gcggcgatga ttacccgtaa agccggtccg gcgctggcgg caggctgcac catggtgctg
     4921 aagcccgcca gtcagacacc gttctctgcg ctggcgctgg cggagctggc gattcgcgcg
     4981 ggcattccgg ctggcgtgtt taacgtagtc accggttcgg caggcgcggt cggtaatgaa
     5041 ctgaccagca acccgctggt acgcaaactg tcgtttaccg gttcgaccga aattggccgc
     5101 cagttaatgg aacagtgcgc gaaagacatc aaaaaagtgt cgctggagct gggcggcaac
     5161 gcgccgttta tcgtctttga cgatgccgat ctcgataaag ccgtggaagg cgcgctggcc
     5221 tcgaaattcc gcaacgccgg gcaaacctgc gtctgcgcca accgtttata cgtgcaggac
     5281 ggcgtgtatg accgctttgc cgaaaaattg cagcaggcgg tgagcaaact gcacatcggc
     5341 gacgggctgg ataaaggcgt caccatcggg ccgctgatcg atgaaaaagc ggtagcaaaa
     5401 gtggaagagc atattgccga tgcgctggag aaaggtgcgc gtgtggtttg cggcggtaaa
     5461 gcggatgaac gcggcggcaa cttcttccag ccgaccattc tggtggacgt gccggccaac
     5521 gccaaagtgt cgaaagaaga gacgttcggc cccctcgccc cgctgttccg ctttaaagat
     5581 gaagccgatg tgattgcgca agccaatgac accgagtttg gccttgccgc ctatttctac
     5641 gcccgtgatt taagccgcgt cttccgcgtg ggcgaagcgc tggagtacgg catcgtcggc
     5701 atcaataccg gcattatttc caatgaagtg gccccgttcg gcggcatcaa agcctcgggt
     5761 ctgggtcgtg aaggttcgaa gtatggcatc gaagattact tagaaatcaa atatatgtgc
     5821 atcggtcttt aactggagaa tgcgaatgaa cagcaataaa gagttaatgc agcgccgcag
     5881 tcaggcgatt ccccgtggcg ttgggcaaat tcacccgatt ttcgctgacc gcgcggaaaa
     5941 ctgccgggtg tgggacgttg aaggccgtga gtatcttgat ttcgcgggcg ggattgcggt
     6001 gctcaatacc gggcacctgc atccgaaggt ggtggccgca gtggaagcgc agttgaaaaa
     6061 actgtcgcac acctgcttcc aggtgctggc ttacgagccg tatctggagc tgtgcgagat
     6121 tatgaatcag aaggtgccgg gcgatttcgc caagaaaacg ctgctggtta cgaccggttc
     6181 cgaagcggtg gaaaacgcgg taaaaatcgc ccgcgccgcc accaaacgta gcggcaccat
     6241 cgcttttagc ggcgcgtatc acgggcgcac gcattacacg ctggcgctga ccggcaaggt
     6301 gaatccgtac tctgcgggca tggggctgat gccgggtcat gtttatcgcg cgctttatcc
     6361 ttgcccgctg cacggcataa gcgaggatga cgctatcgcc agcatccacc ggatcttcaa
     6421 aaatgatgcc gcgccggaag atatcgccgc catcgtgatt gagccggttc agggcgaagg
     6481 cggtttctac gccgcgtcgc cagcctttat gcagcgttta cgcgctctgt gtgacgagca
     6541 cgggatcatg ctgattgccg atgaagtgca gagcggcgcg gggcgtaccg gcacgctgtt
     6601 tgcgatggag cagatgggcg tggcgccgga tcttaccacc tttgcgaaat cgatcgcagg
     6661 cggtttcccg ctggcgggcg tcaccgggcg cgcggaagtg atggacgctg ttgcaccggg
     6721 cggtctgggc ggcacctatg ccggtaaccc gattgcctgc gtggctgcgc tggaagtgtt
     6781 gaaggtgttc gagcatgaaa atctgctgca aaaagccaac gatctggggc agaagctaaa
     6841 agacggattg ctggcgatag ccgaaaaaca cccggagatc ggcgacgtac gcgggctggg
     6901 agcgatgatt gccatcgagc tgtttgaaga tggcgatcac agcaaaccgg acgcaaaact
     6961 caccgccgag atcgtggcac gcgcccgcga taagggcctg attcttctct cctgcggccc
     7021 gtattacaac gtgctgcgca tccttgtacc gctcaccatt gaagacgctc agatccgtca
     7081 gggtctggag atcatcagcc agtgttttgc tgaggcgaag cagtagctat gtccgtttgt
     7141 aagagttaac cgttgctaag ggaatgataa cgatgcagta aaaaataata acgatgaggc
     7201 gaacgacaaa gttaaaaacg actgcggaca gtcccctcgc ccctctggag agagggttag
     7261 ggtgaggggt aaaacacgcg gctaaaccct cccctggccc tctccctaag ggagagggag
     7321 aaaaccctcc cgctttcgtt tttaagattt tgttgaccgt ctcaattaaa cgcgtgaccc
     7381 ggcgcgggaa atgtcggggc gctctcccaa gtgacacact ttcgagagga ttcaggatgg
     7441 ggcaatcatc gcaaccacat gagttaggcg gcgggctgaa atcacgccac gtcaccatgt
     7501 tgtctattgc cggtgttatc ggcgcaagtc tgtttgtcgg ttccagcgtc gccatcgccg
     7561 aagcgggccc ggcggtatta ctggcctatc tgttcgccgg gctactggtg gttatgatta
     7621 tgcggatgtt ggcggaaatg gcggttgcca cgcccgatac cggttcgttt tccacctatg
     7681 ccgataaagc cattggtcgc tgggcgggct ataccatcgg ctggttgtac tggtggtttt
     7741 gggtactggt tatcccgctg gaagccaata tcgccgctat gatcctgcac tcgtgggttc
     7801 caggcattcc catctggtta ttttccctcg tcattaccct tgccttaact ggcagtaatt
     7861 tattaagcgt taaaaactac ggcgaatttg agttctggct ggcgctgtgc aaagtcatcg
     7921 ctatcctggc ctttattttc cttggtgcag tcgcaattag cggtttttac ccttatgccg
     7981 aagtgagcgg gatctcaaga ttgtgggata gcggcggctt tatgcccaac ggtttcggtg
     8041 cggtattaag cgcgatgttg atcaccatgt tctcgtttat gggcgcagaa attgtcacca
     8101 ttgccgccgc ggaatccgac acgccggaaa aacatattgt ccgcgccacc aactcggtta
     8161 tctggcgtat ttctatcttc tatttgtgct ctatttttgt cgtcgtggca ttaataccgt
     8221 ggaatatgcc ggggctgaaa gccgttggat cttatcgctc ggttctggaa ttgctcaata
     8281 ttccccatgc gaaattaatc atggactgtg tgatattact ttccgtaacc agttgcctga
     8341 actcggccct gtataccgcc tcaaggatgc tctactcctt aagccgtcgc ggtgatgctc
     8401 ccgcggtaat gggtaaaatc aaccgcagta aaaccccgta cgtggcggtg ttactctcca
     8461 ccggcgcggc attcttaacg gtggtggtga actattacgc gcctgcgaag gtatttaaat
     8521 ttctgatcga cagctccggc gctatcgccc tgctggttta tttagtcatc gccgtttcac
     8581 agttgcggat gcgcaaaatt ctgcgagcgg aaggaagcga aattcgcttg cgaatgtggc
     8641 tttatccctg gctcacctgg ctggtcatcg gctttattac ctttgtgttg gtagtgatgc
     8701 tattccgtcc ggcgcaacag ttagaagtga tctccaccgg cttattagcg atagggatta
     8761 tctgtaccgt gccaattatg gctcgctgga aaaagctggt attgtggcaa aaaacacccg
     8821 ttcataatac gcgctgataa tgatcaggag tcacaccatg accattacgt ctctggatgg
     8881 ctatcgctgg ctgaagaacg atattattcg cggtaatttt caaccggatg aaaaattacg
     8941 gatgagtttg ctgacatcgc gttatgcact tggcgttggg ccgttgcggg aagcactttc
     9001 tcaactggtg gcggaacggc tggtcacggt ggtgaatcaa aaagggtatc gggtggcgtc
     9061 tatgtcagag caggagctgc tcgatatttt cgatgcccgc gccaatatgg aagcgatgtt
     9121 agtgagtctg gcgattgccc gcggtggcga tgagtgggag gcagacgttc tcgcaaaagc
     9181 gcatctgctc agtaagctgg aagcctgtga cgccagcgag aaaatgcttg atgagtggga
     9241 tctgcgtcat caggcgtttc atacagcaat tgtggcgggc tgtggttctc actatttgct
     9301 gcaaatgcgt gaacggttgt ttgatctggc ggcgcgctat cgatttatct ggctgcggcg
     9361 aacggtgctt tcggtagaaa tgctggagga taaacacgat cagcaccaga ccctgactgc
     9421 ggcggtactg gcgcgagata ccgcgcgcgc cagtgagtta atgcgccagc atttactgac
     9481 gccaattccc attatccagc aggcgatggc tggcaattaa ctactcttcc ggaatacgca
     9541 acacttgccc cggataaatt ttatccgggc tttttagcat cggtttattc gcttcgaaga
     9601 ttttattgta cagattagcg ttaccgtaga cctgtttgga aatggcactc agagtgtcgc
     9661 cagacttaac ggtataaaac tggctggcag tggctggtgt cgccgttttc acctgatcat
     9721 caacactggc aataccggaa atattcccca ccgcaacaag gattttctcc ttcgcctcct
     9781 gactcaggcc gtcaccagtg accgtcgctt tgccgtcggc aatttgaatg ttcactttat
     9841 cagcatcagg tataccggtt ttgctcagat gctcctgcac cttcttcgcc tgatcgtctt
     9901 tatcgtgctg acctgtaacc gcgtcccaga gtttttctcc ggcatctttc acaaaattga
     9961 acagacccat agctacctca ttcgttaacg gaaacactca gaaagtgtag cagaggaacg
    10021 cggcgcgtta acgccgcggg ggtgagcaca ggtttaatcg cgggtttgca cccagaacgc
    10081 gtgaatcaaa ccggggatat acccaagcag tgtcagcaga atattaataa tgaacgccca
    10141 accgaaccct ttaccgagca gcacgccgag cggcggtaga atgatggtga tgacgattct
    10201 ccagaaaccc atatgtactc cctataagaa aattactcat tgtttaaaaa gagattttat
    10261 ctcttaagcg tagtaatttt agcggagcct gccagtttca gcttacgctt ttactctcat
    10321 ttacgctgct gaggctggta tatgttgcat ttttagttta gagcttactt aatttagaaa
    10381 atacttaaat aaatatgact gaactcgcgc aattacaggc cagtgccgaa caggcagcgg
    10441 ccttattgaa agcaatgagc catcctaaac gattgctgat tctgtgcatg cttagcggtt
    10501 cccccggcac cagcgcggga gagctgacgc gcattaccgg actgagtgcc tctgcgacat
    10561 cacagcatct cgctcgtatg cgggacgaag ggcttatcga cagccaacgg gatgcccaac
    10621 gcattctata ttccattaaa aatgaggcgg taaatgccat tatcgccacc ctgaaaaatg
    10681 tctattgtcc gtaaggagtc actatggctt tgacaaccat ttcgccgcat gatgcacaag
    10741 aattaatcgc acgcggcgca aagttaatcg atattcgtga cgctgatgaa tatcttcgtg
    10801 aacatattcc tgaagcagat ctggctccat tatccgtgct ggaacagtca ggtcttccac
    10861 ctaaattacg tcgcgagcaa attatattcc actgccaggc aggtaaacgc accagtaata
    10921 acgccgataa attagcagcg attgccgccc ccgcagaaat atttttactc gaagatggga
    10981 ttgatggctg gaaaagagct ggattgccag tagcggtaaa taaatctcaa cccttgccgt
    11041 taatgcgcca ggtgcagatc gctgcgggtg gtttaatatt aatcggcgtt gtactgggtt
    11101 ataccgtaaa tagcggtttc ttcttattaa gtggctttgt tggtgccggg ttactgtttg
    11161 caggaatcag cggtttttgt ggaatggcaa ggttgttaga taagatgccg tggaaccaac
    11221 gagcttgaga agcgacgccg gacgcgccct agcagcgaca tccggcctca gtaattagat
    11281 caggaaatcg tcgagagatt taccttctgc cagcgcctga gcaattggct tcggtgtacg
    11341 gccctgaccg gtccaggttt tagtttcacc gttaacatcg gtgaatttat atttcgccgg
    11401 acgcggctgg cgttttttac cagcgcgtgg tgcagcagca gagctattac ccaataactc
    11461 ttccgggtta attccgtcag ccttcatcag ctccagccag gtgctaattt tttcctgacg
    11521 ctcagccagt tcacgctgct gctgttcttc ttcttcacgt ctttctttag tgacaaccct
    11581 gaatttttcg agcatttctt caagaacgtc aatggagaat tcgcgagcca tcgcacggag
    11641 ggtgcgaatg ttatttaaac tttgtaacat tacggacata ataaagaaaa ccttttaacg
    11701 ccaaaacaaa aaagtatttc gtcgcgtagt gtaagcgatc acttattata tttccacaaa
    11761 atttaatcta tttacagtct tgcgcgagat tatttcagct gttattttct gaaactctca
    11821 gaataatttc aagattatat attagccatt ttgatgagta atttagatta ttggtgaaca
    11881 gaacggcgct ctctgctcgc taattccgca agcctgacaa aaccgaagtg aataaaaatg
    11941 caatataaca gaataaaaga
//
LOCUS       DAJVQG010000119        11877 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJVQG010000119 DAJVQG010000000
VERSION     DAJVQG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.119"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            486..1157
                     /locus_tag="OQM83_003001"
     CDS             486..1157
                     /locus_tag="OQM83_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HCS1521207.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCNQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            1167..2363
                     /locus_tag="OQM83_003002"
                     /pseudo
     CDS             1167..2363
                     /locus_tag="OQM83_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            2363..2923
                     /locus_tag="OQM83_003003"
     CDS             2363..2923
                     /locus_tag="OQM83_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCS1521208.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2925..3698
                     /locus_tag="OQM83_003004"
     CDS             2925..3698
                     /locus_tag="OQM83_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HCS1521209.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3886..4161
                     /gene="frmR"
                     /locus_tag="OQM83_003005"
     CDS             3886..4161
                     /gene="frmR"
                     /locus_tag="OQM83_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HCS1521210.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            4196..5305
                     /gene="frmA"
                     /locus_tag="OQM83_003006"
     CDS             4196..5305
                     /gene="frmA"
                     /locus_tag="OQM83_003006"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HCS1521211.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5398..6231
                     /gene="fghA"
                     /locus_tag="OQM83_003007"
     CDS             5398..6231
                     /gene="fghA"
                     /locus_tag="OQM83_003007"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCS1521212.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6457..6996)
                     /locus_tag="OQM83_003008"
     CDS             complement(6457..6996)
                     /locus_tag="OQM83_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HCS1521213.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(7098..8309)
                     /gene="mhpT"
                     /locus_tag="OQM83_003009"
     CDS             complement(7098..8309)
                     /gene="mhpT"
                     /locus_tag="OQM83_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HCS1521214.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALALLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8596..9609)
                     /gene="dmpG"
                     /locus_tag="OQM83_003010"
     CDS             complement(8596..9609)
                     /gene="dmpG"
                     /locus_tag="OQM83_003010"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HCS1521215.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9606..10556)
                     /gene="mhpF"
                     /locus_tag="OQM83_003011"
     CDS             complement(9606..10556)
                     /gene="mhpF"
                     /locus_tag="OQM83_003011"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HCS1521216.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10553..11362)
                     /gene="mhpD"
                     /locus_tag="OQM83_003012"
     CDS             complement(10553..11362)
                     /gene="mhpD"
                     /locus_tag="OQM83_003012"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HCS1521217.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11372..>11877)
                     /gene="mhpC"
                     /locus_tag="OQM83_003013"
     CDS             complement(11372..>11877)
                     /gene="mhpC"
                     /locus_tag="OQM83_003013"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HCS1521218.1"
                     /translation="AFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQLYRQP
                     TIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFPDFGPR
                     LAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADAFNQLV
                     LNFLARP"
BASE COUNT         2912 a         3177 c         2908 g         2880 t
ORIGIN      
        1 aatatgcaat tactccgttc taacaattgg gaaaattact acacttcatt ttagtgatgc
       61 ataaacaacc acattacgag atttattact tcattgtttc tatcaataca tataataaaa
      121 ctattatatt cgcattgtat atatcactgt atcattctca atcactcgca tattacattc
      181 acataatgta tataaacaat acttgttcac agaacatatt ttttaaatgc aattaattga
      241 caaactaaat cacatctaaa tacaatgtat aaaacaaaat aactatacat accaaacata
      301 caaacactta acatatgaat tgcataaaaa cattacccag tcgaacctcc gggtctgaaa
      361 gcccttgctt ttcagttatt agcaacattt aacatttata aattaacatc tggctgcgcc
      421 agagattacg tctctgagtg catcgttgtg cttgtagtat aataaataaa atggagtgtc
      481 attaagtgga taaggtttta gattcagccc tcctttcttc ggcaaataaa agaaagggta
      541 tccttgctat tggcgcacat cctgacgata tagaattagg ctgtggcgca tcgcttgctc
      601 gtcttgcgca aaaaggaatt tatatcgcag cagtggtgat gactaccggc aactctggta
      661 cagatggaat aatcgatcgc catgaagaat cgcgcaacgc cctaaagata ttagggtgca
      721 accaaactat tcatcttaat tttgctgaca cccgcgctca tttacagctc aatgatatga
      781 tttccgccct ggaagacatc attaaaaatc aaattccttc tgatgttgaa atcatgcggg
      841 tatataccat gcatgatgcc gaccgccatc aggatcatct cgctgtttat caagcttcaa
      901 tggttgcctg ccgcactatt ccacaaattc tcggctacga aaccccaagt acctggcttt
      961 catttatgcc tcaggttttt gaatccgtta aagaagaata tttcacagtc aaacttgctg
     1021 cattaaaaaa acataaaagc caggaacgac gtgattatat gcgtcatgat cgcctgcgtg
     1081 cagttgcaca atttcgcggg caacaggtca atagcgatct gggtgaaggc tttgtaattc
     1141 ataaaatgat tctttgagca aattgcatga aaacctggat atttatctgt atgtccatag
     1201 caatgttgct atggttttta agtacgctaa gacgtaaacc cagtcaaaag aaaggctgta
     1261 ttgacgccat tatacctgcg tataacgaag gcccgtgtct ggcgcagtca ctggataatc
     1321 tactgcgtaa cccttatttt tgccgggtaa tttgcgttaa cgacggctcc acggacaata
     1381 ccgaagcggt catggcggaa gtcaaacgca aatggggcga ccgctttgtt gccgtcacgt
     1441 aaaaaaatac cggtaaaggt ggtgcgctga tgaatggcct caattatgcc acctgcgacc
     1501 aggttttttt aagtgatgcc gacacctatg ttccgcccga tcaagacgga atgggctata
     1561 tgctggcaga aattaagcgc ggtgctgatg ccgtaggcgg cattccctct actgcgttga
     1621 aaggcgcggg gctgttaccg cacatccgcg cgaccgtaaa gttgccgatg attgttatga
     1681 agcgcacgct acagcagctc ctgggcggcg caccgtttat tatcagcggt gcctgcggaa
     1741 tgttccgtac tgatgtattg cgtaagttcg gtttctcgga tcgtactaaa gtcgaagacc
     1801 ttgatctcac ctggacatta gtggcaaacg gctaccgtat tcggcaggcg aatcgctgca
     1861 tcgtataccc acaggaatgc aacagtccgc gtgaggagtg gcgtcgctgg cggcgttgga
     1921 ttgtgggcta cgcggtctgt atgcgcctgc ataaaaggct tttatttagc cgcttcggta
     1981 tcttcagtat atttcctatg ctgttggttg tgatttatgg cgttgggatt tatctcacta
     2041 cctggtttaa tgaattcatc accaccgggc cgcatggtgt ggtgttggca atgtttccgc
     2101 ttatctggat cggcgtagtt tgtgttattg gtgcttttag cgcctggttt catcgttgct
     2161 ggttgttggt gcctttagcg ccgctttccg ttgtgtatgt attattagct tatgccatct
     2221 ggattatcta tggacttatt gcctttttta ctggacgcga acctcagcgc gacaaaccca
     2281 cccgctattc cgcactggtg gaagcgtcaa ccgcttattc ccaaccttct gtcacaggaa
     2341 ctgaaaaact atctgaagct taatgttaaa gagaaaaatt attcagattg cagaccaggt
     2401 aattattgat gaaactgcag gtgaagttgt tatcggcgcg aatacccgta tttgtcatgg
     2461 tgccgttatt cagggtccgg tagtgattgg cgcaaactgc ctgataggta attatgcgtt
     2521 tattcgtcct ggcacaataa tcagcaatgg tgtaaaaatt ggttttgcca ccgaaattaa
     2581 aaatgcggtt attgaagcgg aagcaacgat tggtccgcaa tgttatattg ccgactcggt
     2641 agttgcaaac caggcatatt tgggcgcaca agtacgtacc agtaatcatc gtctggatga
     2701 acagcctgtg tctgttcgaa ctccagaggg aattatcgct accggatgcg ataaattagg
     2761 ttgttatatc gggcagcgtt cacgccttgg cgtacaagtt attattttgc ctgggcgaat
     2821 tatttccccg aacacgcaac ttggcccgcg cgtgattgta gaacgtaatt tacctaccgg
     2881 aacttattca ctccgacaag aacttatccg tacaggagat taaaatgata aaacggacgt
     2941 tattagcggc ggccattttt agcgcattgc ccgcttatgc cgggttaact tccattaccg
     3001 cgggctacga ttttaccgat tattctggcg atcatggcaa ccgtaattta gcgtatgctg
     3061 aactggtggc gaaagttgaa aacgcaacgc tgctttttaa tctttcccag gggcgtcgtg
     3121 attatgaaac tgaacatttc aatgccactc gcggtcaggg tgcggtctgg tataaatgga
     3181 ataactggct gacaacccga acgggtattg cctttgcgga taatacgccg gtctttgccc
     3241 gccaggattt tcgtcaggat attaacctgg ccctgttgcc aaaaacgctt ttcacgaccg
     3301 gttatcgcta cactaaatat tacgatgatg tcgaagtcga tgcctggcaa ggcggcgtat
     3361 cactctatac tggcccggtg atcaccagct accgctatac ccattatgac tccagcgatg
     3421 caggtggtag ttatagcaat atgatttccg tgcgtctgaa tgacccgcgc ggcactggtt
     3481 atacgcaact atggctaagc cgcggaacag gcgcttacac ctatgactgg acgccagaaa
     3541 cacgctacgg cagcatgaag agcgtcagtt tgcaacgtat tcaaccgctg actgagcaac
     3601 ttaatctcgg gctgacggca ggtaaagtgt ggtacgacac cccaaccgat gattataacg
     3661 gtctgcaact tgcagcccat ctgacctgga aattctgatt gcttctgccg cccgctatcc
     3721 ggggcggcct tccctgccga ttagccccac tccctttcct cgttgttttc cgaccacatt
     3781 caccggataa attttattct ccagcgttat atactatagg gaggtatgca ttgacatata
     3841 gcatacctcc ctatagtata ttgcgtgcag atgatgaggt gcgaaatgcc cagtactccg
     3901 gaagagaaga aaaaggtcct tactcgagtt cgtcgtattc gggggcagat tgatgctctg
     3961 gaacggtcgc tggagggtga tgccgaatgc cgtgccatac tccaacagat cgctgccgtt
     4021 cggggcgcgg ctaacgggct gatggcagaa gtgcttgaaa gccatatccg ggaaacgttt
     4081 gaccgaaatg actgctacag ccgcgaagtc agccaatccg ttgacgacac tattgaactg
     4141 gttcgagcct atcttaaata gctgaatcta ttaccatatt gaggaagagc gagagatgaa
     4201 atcacgtgct gccgttgcat ttgctcccgg taaaccgctg gaaatcgttg aaattgacgt
     4261 tgcaccaccg aaaaaaggtg aagtgctgat taaagtcacc cataccggcg tttgccatac
     4321 cgacgcattt accctctccg gggatgaccc ggaaggtgta ttcccggtgg ttctcggtca
     4381 cgaaggggcc ggcgttgtgg ttgaagtcgg tgaaggcgta accagcgtca aacctggcga
     4441 ccatgtgatc ccgctttaca ccgcggagtg cggcgagtgt gagttctgtc gttctggcaa
     4501 aactaacctc tgtgttgcgg ttcgcgaaac ccagggtaaa ggcttgatgc cagacggcac
     4561 cacccgtttt tcttacaacg ggcagccgct ttatcactac atggggtgct ctacattcag
     4621 tgaatacacc gtggtcgcgg aagtgtctct ggccaaaatt aatccagaag caaaccatga
     4681 acacgtctgc ctgctgggct gtggcgtgac caccggtatt ggcgcggtgc acaacacagc
     4741 taaagtccag ccaggtgatt ctgttgccgt gtttggtctt ggcgcgattg gtctggcagt
     4801 ggttcagggc gcgcgtcagg cgaaagcggg acggattatc gctatcgata ccaacccgaa
     4861 gaaattcgat ctggcgcgtc gcttcggtgc taccgactgc attaacccga atgactacga
     4921 caaaccgatt aaagatgtcc tgctggatat caacaaatgg ggtatcgacc atacctttga
     4981 atgcatcggt aacgtcaacg tgatgcgtgc ggcgctggaa agtgcgcacc gcggctgggg
     5041 tcagtcggtg atcatcgggg tggcaggttc tggtcaggaa atctccaccc gtccattcca
     5101 gttggtcact ggtcgcgtat ggaaaggttc cgcgtttggc ggcgtgaaag gtcgttccca
     5161 gttaccgggt atggttgaag atgcgatgaa aggtgatatc gatctggaac cgtttgtcac
     5221 gcataccatg agccttgatg aaattaatga cgccttcgac ctgatgcatg aaggcaaatc
     5281 cattcgaacc gtaattcgtt actgatttcc cgcaggtata ccccgtccac ttcagacggg
     5341 gttcttaata ctctccccgg gcagccgtcc gggggattaa ccgtgagata atgactgatg
     5401 gaactcattg aaaaacatgc cagctttggc ggctggcaaa atgtgtatcg gcattattcc
     5461 caatcactga aatgtgaaat gaatgtcggc gtctatctcc caccaaaagc cgcgaatgaa
     5521 aaattgccgg tgctgtactg gctttcaggc ctgacctgca acgagcagaa tttcattact
     5581 aaatcgggga tgcagcgtta cgcggctgag cacaacatta ttgttgttgc gccggacacc
     5641 agtccgcgag gcagtcatgt cgcagatgcc gaccgttacg atctcgggca aggtgccggg
     5701 ttttacctga acgcaacgca agcgccgtgg aatgaacatt acaaaatgta tgactatatc
     5761 cgcaacgagc tgccggattt agtgatgcaa cattttccgg caacgaccag aaagtctatc
     5821 tctggtcatt ctatgggcgg gctgggcgcg ctggtgctgg cgttacgtaa cccagatgaa
     5881 tatgtcagcg tctcggcgtt ttcgcccatt gtctccccat cgcaagtacc gtggggacag
     5941 caagcctttg ccgcatatct tggtgaaaat aaagatgcct ggttggatta cgatccggtg
     6001 agtcttattt cacaaggtca acgcgttgcg gaaatcatgg ttgatcaggg gttgagtgat
     6061 gatttttacg cggaacagct gcggactcca aatcttgaaa agatctgcca ggagatgaat
     6121 atcaagacgt taatccgtta tcacgagggt tacgaccaca gctattattt tgtctccagt
     6181 tttattggcg agcatattgc ctaccacgcc aataaactga atatgcgttg ataatagtgc
     6241 acgactgccg gatgcggcgt gaacgcctta tccggcctac acttcgcccg taaaccgtag
     6301 gcctgataag acgcgcaaag cgtcgcatca ggcatggcgc acgactgccg gatgcggcgt
     6361 gaacgcctta tccggtctac acttcgccca taaaccgtag gcctgataag acgcgcatag
     6421 cgtcgcatca ggcatctgcg cacgacttat ctatgtttac cacatcaaat catcaggcac
     6481 tttgaagtcg gcatacggat cgtcttcatc ctgctcttcg gcactgagcg cgctgtgcaa
     6541 cacaatactg ctggcatcac gctgagcaat tttatcggca acactggcgg gaataatggt
     6601 gtattcacct tcgctattgt tatcgaccaa caagcgggca atcgccagac gaccattaat
     6661 caactgcgcc tgtgtgagct tatcgacaaa aatcttctta atcagattgc cgtcagtgaa
     6721 gttaaaacca atatcgccat tggcaatggt gattcggttc atttcaatga gctgcttcac
     6781 ctgggcttta tattctttcg ccaacgccgc ttgtttttgc tgttcgctaa gctgtttatc
     6841 acgctcaagc tgtgcctttt tattttcttc taccgcagcc cgagcttcac gcgcctgaac
     6901 gcgcgatttt ttcgccgttc tctcgacctt cgccgctttt ttgctggtga ctaatcctgc
     6961 tttgagcaac tgctcttgta aggtaagttt tgccatcttt gtttccaaac ccgctgaata
     7021 atttttagcg agtatacctg taaacgccgt gggtgtattg ggggcaagtc cctttcgccc
     7081 ctgcttaata aggcgcgtca ggcatcggca cacggctgca ttcttgatct ccgactcatc
     7141 aaaataaaca ccgccaaccc agcaacaaga atacccggtg cagaagcggc cattacgccg
     7201 accgtgccag tgcctaatgc cagcattttc ccggccagta acggaccgct catagccccc
     7261 agacgcccta ccgccacggc tgttcccaca cctgttgcgc ggatctgcga actgtaaaac
     7321 aacagtgcca gggcatacaa aacgctttgc ccacctgtcg caaacaaccc cgcgacaaat
     7381 cccgccagca acataccgtt aaatgacgac accgttccaa gcgccagcag cgaagctaac
     7441 atgccgctat aaatcagtag cgacatggtt actggacgca gcttatccat caatgcgccc
     7501 aacattaacg tcccgcttgc cgcccccatt tgcagggcaa acatcacccc tgccgcctgc
     7561 gatggctgga atccttgctc caccaaaagt agcggtagcc agttgatcaa catataaacc
     7621 accagcagag tgaagaaata acacaaccac agcagcagcg tcgcggttgc cgtttctggc
     7681 gcaaataagg cacgcagtgg tggcgcagcc tgtttttcgc cagcgaaaac cgctgactcc
     7741 ggcagccagc gcattaatag cggcaccaga atcaacggca ccacaccacc tacccaaaac
     7801 accgtttgcc atgctaagtt tgcccccgcg aaacccagtg tcgccgccag cgccgcgcca
     7861 atgggaacac cgcaatacat caggctcact gccgtcccac gaaaacgtgg acccgcggct
     7921 tcagatgtca gggcgataag attcggcaac gccgccccca gcccgacacc ggtcatcagc
     7981 cgcgcaaaga ccagtgaggg gaaatcccag gcaatcgccg ttgccagtga gaacaaacca
     8041 aacagcgcaa ctgagccaat caaaatacgc ttacgaccat aacggtctgc cagcatcccg
     8101 ccaaccaacg cgccgggtag caaaccgagt attccggcgc taaatatcca gcccatttgc
     8161 attttatcga gtgcgaaagc ctgggcgatg ccacccgccg caatgccagc cgcctgaaga
     8221 tccagccctt ccatcagagc gaccaaaaaa caaagcccga tggtcagcat caggcgggat
     8281 gaagatgatg aaggggtacg agtcgacata agatgaacct cattgcgagt gcaggtgaat
     8341 cgcgactgat acattgccag ccgcgtaatc agaactattt aatgactgcc tacgggcgaa
     8401 atgtagcgaa atgtaggccg gataaggcgt tcacgccgca tccggcagtc gtgcgcagat
     8461 gcctgatgcg acgctaacgc gtcttatcag acctacaaat tctcacgcca tccgtaggcc
     8521 ggacaaggcg ttcacgccgc atccggctgt cgtgctggga tgggcataca aaatgcacgt
     8581 tatcggcagt catcattatt tgttgttgcg cagatccagc gccacgtcga cgatcatatc
     8641 ctcctggccg ccaaccatcc ggcgtttgcc cagctcaacg agaatatcca ccgcacttaa
     8701 gccataacgc gccgccgccg tttcacagtg acgcaggaag ctcgagtaaa caccagcgta
     8761 tcccagcgcc agcgtttcgc gatcgactcg taccggacga tcctgcaacg gacgcaccag
     8821 gtcgtcggcg gcatccatta acgcatagag atcggtccca tgctgccagc ccagtttatc
     8881 cgcggcggca ataaacactt ccagcggtgc gttacctgcg cccgcgccca ttcccgcgag
     8941 gctggcgtcg attcggtcgc agccctcttc caccgccgcg atagagttcg ccacgccaag
     9001 actcaggtta tggtgagcgt gcatgccggt ttgcgtttct ggtttcaaca ctgctttcag
     9061 ggcgcggaaa cggtcacgga tatcgctcat gttcatcgca ccgccagaat ccaccacata
     9121 aatacaggtc gcaccgtagc cttccatcag ctttgcctgc ttggcgagat tctccggcgt
     9181 ggtcatatgg ctcatcatca gaaaaccaac ggtgtccatt ccgagctcgc gggcatactg
     9241 aatatgctgg gcggaaacat cagcttcggt acagtgcgtt gccacacgaa ccacccgcgc
     9301 gccagcctgc caggcatttt tcagatcgtg aatagtgccg attcctggca gcaacaacgt
     9361 cgcgattttg gcgtgcctca ccacatccgc cgccgcttca atccattcaa ggtcgctatg
     9421 tgcgccgaaa ccatagttaa agctggaacc ttgcaaaccg tcgccgtggg ccacttcaat
     9481 cgaatccacg cgggcatcgt ccagtgcttt ggcaatctgg cgaacgtttt ccagcgaata
     9541 ctgatgacga atggcgtgca taccgtcacg caatgtgacg tccgagatat aaagtttttt
     9601 accgttcatg ccgcttctcc tgccttgcgc gccagtgact gggccatttt ttccgctgtc
     9661 gccagcgcac tggaagtcat aatatcgagg ttgcccgcat aggcaggcag ataatgcgct
     9721 gcgccttcga cttccagcca gaccgctgtt ttcagtccgg agaattgccc cacgcccggt
     9781 aaattgaccg gtttatcctg cgggataact tcaaactgca cgcgctgttt caggcgataa
     9841 cccggtacgt aagcctgcac cgcctcagcc atttcattga ttgaggcttc gatatcatct
     9901 tgtgaagctt cgtcgctcaa tacatacacc gtgtcacgca tcatcaacgg tggctctgct
     9961 gggttaagca caataatcgc cttccctttt gccgcgccgc ccaccacttc aatggctcgg
    10021 gaagtggttt ccgtaaattc atcgatattg gcacgcgtgc caggtccggc agatttactg
    10081 gcgatagaag cgataatttc ggcgtaatga acacgcgcca cgcgtgaaac tgccgccacc
    10141 attggaatgg tggcctggcc gccgcaggtg accatgttga cgttcagttg atcgacgttc
    10201 gcctcgaggt taaccaccgg cacgcagtaa gggccgatgg cagcaggcgt caggtcaatt
    10261 aagcgaatat ccggtttcgc ttcgcgtaaa gcggcatcgt ttttcacatg cgcgcccgcg
    10321 ctggtcgcat caaatacaat gtcgatatca gcaaattcag gcatgttcat cagcccgatc
    10381 accccttcat gggtggtggc gacgcccata cgtctggcac gcgccagacc gtcggactga
    10441 ggatcaatgc cgaccatcac cgccatctcc agatgctgac cgtgacgcaa aattttaatc
    10501 atcagatcgg tgccaatgtt gccagaaccg ataatggcga ctttacgctt actcatgaca
    10561 gacttccttt tggggctgcg cttgaaaatg tcgcagcaac tgaacctatg ccttcaatat
    10621 gggcttcaaa acgatcgccc gcattcaccg ccaccatcgg gcctaatgcc ccggtaagaa
    10681 tgatatctcc ggcgcgcagc ggttcaccca gactggccat tttgcgtgcc agccagacgg
    10741 cggcattaag cggatgtccc aggcattcgc tgccgcgccc gctggaaacc tcttcgttat
    10801 tacgcgtcat cttcatggcg cagtttttca ggtctaaccc cgccggacgt tgcgccggac
    10861 cgccgatgac atacaccccg caggaagcgt tatctgccac ggtatcgaca aactgaatcg
    10921 accagtcgcg aatgcggctc cccaccactt ccagcgccgg aagtacccat tcaatggcgt
    10981 tatacagttc gtcgaaggtg atatcagttg cgggtaaatc gcggttcaac accagtgcga
    11041 tctccgcttc aatgcggggt tgcagaacac gggaaaaagg aatgatttcg ttatcgccat
    11101 aacacatgtc ggcaaataac gtcccaaaat ccggttgatc aacgcccagt tgttgttgca
    11161 ctttcggatg cgtcaggccc actttacgcc ctaccacgcg acgcccctgc acaacgtcat
    11221 attgcacatt tatgtgctga atggcgtagg ccgcttcagc gtgatcgata ccaatcagat
    11281 cgcgcagcgg cgcaatcgct tcgccctgct ctgcggcgcg gcgtaaatcc gccgccagtt
    11341 gctcaagagt atgcttcgtc atgaccattc cttaagggcg tgcgaggaaa ttcagcacca
    11401 attgattgaa agcgtcggca tgttcccact gcgcccagtg accacagtcg cggaagatat
    11461 gcagttcaga accggcaatg ccggacagca gacgcagacc cgcatccatc ggcacaaagc
    11521 ggtcgttgcg cccccagaca atcagggttt gcgctttgat ttccgccaga cgtgggccaa
    11581 aatccgggaa ctgtttcgga ttagcttcca ggctcttaac gaagttttcc aggtgatcgc
    11641 ggcgcgacag catattattc aggcgcgctt caaacagggc gtcggtcaaa tcgctggtat
    11701 cgaaaacgaa gatatccatc atcagcttca ggttttcgat agtcggctga cgataaagct
    11761 gattcagtcg cttaatacct tcggttggca tcggcgtaaa caaactcatg ccgcccgtac
    11821 cgccgcccat cagcaccagt ttgccgacgc gctccggcca gttaagggtg aacgcca
//
LOCUS       DAJVQG010000120        11823 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJVQG010000120 DAJVQG010000000
VERSION     DAJVQG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11823
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.120"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..1660
                     /gene="maeA"
                     /locus_tag="OQM83_003014"
     CDS             <1..1660
                     /gene="maeA"
                     /locus_tag="OQM83_003014"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCS1521219.1"
                     /translation="PYAGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQA
                     ERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERF
                     SEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPI
                     GKLSLYTACGGISPAYTLPVVLDIGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFI
                     QAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAA
                     GGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFMVDRFGLLTDKMPN
                     LLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQTGLFTEEIIREMH
                     KHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVVWKDKIYPIAQCNN
                     AFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLVLPELKDIQKVSRA
                     IAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1794..2796
                     /gene="adhP"
                     /locus_tag="OQM83_003015"
                     /pseudo
     CDS             1794..2796
                     /gene="adhP"
                     /locus_tag="OQM83_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            2942..3226
                     /locus_tag="OQM83_003016"
     CDS             2942..3226
                     /locus_tag="OQM83_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCS1521220.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(3454..4107)
                     /gene="fdnI"
                     /locus_tag="OQM83_003017"
     CDS             complement(3454..4107)
                     /gene="fdnI"
                     /locus_tag="OQM83_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCS1521221.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4100..4984)
                     /gene="fdnH"
                     /locus_tag="OQM83_003018"
     CDS             complement(4100..4984)
                     /gene="fdnH"
                     /locus_tag="OQM83_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCS1521222.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(4997..8044)
                     /gene="fdnG"
                     /locus_tag="OQM83_003019"
     CDS             complement(4997..8044)
                     /gene="fdnG"
                     /locus_tag="OQM83_003019"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7457..7459),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCS1521223.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8276..9157
                     /gene="yddG"
                     /locus_tag="OQM83_003020"
     CDS             8276..9157
                     /gene="yddG"
                     /locus_tag="OQM83_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCS1521224.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9416..10516
                     /gene="ompC"
                     /locus_tag="OQM83_003021"
     CDS             9416..10516
                     /gene="ompC"
                     /locus_tag="OQM83_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCS1521225.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            10680..11162
                     /locus_tag="OQM83_003022"
                     /pseudo
     CDS             10680..11162
                     /locus_tag="OQM83_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11277..>11823
                     /locus_tag="OQM83_003023"
     CDS             11277..>11823
                     /locus_tag="OQM83_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCS1521226.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAH"
BASE COUNT         3037 a         2968 c         2824 g         2994 t
ORIGIN      
        1 cccttacgct ggccctgtac tgctggaatt tccgttgttg aataaaggca gtgccttcag
       61 catggaagaa cgccgtaact tcaacctgct ggggttactg ccggaagtgg tcgaaaccat
      121 cgaagaacaa gcggaacgag catggatcca gtatcaggga ttcaaaaccg aaatcgacaa
      181 acacatctac ctgcgtaaca tccaggacac caacgaaacc ctcttctacc gtctggtaaa
      241 caatcatctt gatgagatga tgcctgttat ttatacccca accgtcggcg cagcctgtga
      301 gcgtttctct gagatctacc gccgttcacg cggcgtgttt atctcttacc agaaccgcca
      361 caatatggac gatattctgc aaaacgtacc gaaccataat atcaaagtga ttgtggtgac
      421 tgacggtgaa cgtattctgg ggcttggtga ccagggcatc ggcgggatgg gcattccgat
      481 cggtaaactg tcgctctata ccgcctgtgg cggcatcagc ccggcgtata cccttccggt
      541 ggtgctggat atcggaacga acaaccaaca actgcttaac gatccgctgt atatgggctg
      601 gcgtaatccg cgtatcactg acgacgaata ctatgaattc gttgatgaat ttatccaggc
      661 tgtgaaacaa cgctggccag acgtgctgtt gcagtttgaa gactttgctc aaaaaaatgc
      721 gatgccgtta cttaaccgct atcgcaatga aatttgttct tttaacgatg acattcaggg
      781 cactgcggcg gtaacagtcg gcacactgat cgcagcaagc cgagcggcag gtggtcagtt
      841 aagcgagaaa aaaatcgtct tccttggcgc aggttcagcg ggatgcggca ttgccgaaat
      901 gatcatcgcc cagacccagc gcgaaggatt aagcgaggaa gcggcgcggc agaaagtctt
      961 tatggtcgat cgctttggcc tgctgacgga caagatgccg aacctgctgc ctttccagac
     1021 caaactggtg cagaaacgcg aaaacctcag tgactgggat accgacagcg atgtgctgtc
     1081 actgctggat gtggtgcgca atgtaaaacc agatattctg attggcgtct caggacagac
     1141 cgggctgttt acggaagaga tcatccgtga gatgcataaa cactgtccgc gtccgatcgt
     1201 gatgccgctg tctaacccga cgtcacgcgt ggaagccaca ccgcaggaca ttatcgcctg
     1261 gaccgaaggt aacgcgctgg tcgccacggg cagcccgttt aatccagtgg tatggaaaga
     1321 taaaatctac cctatcgccc agtgtaacaa cgcctttatt ttcccgggca tcggcctggg
     1381 ggttattgct tccggcgcgt cacgtatcac cgatgagatg ctgatgtcgg caagtgaaac
     1441 gcttgcacag tattcgccgc tggtgctgaa cggcgaaggt ctggtactac cggaactaaa
     1501 agatattcag aaagtctccc gtgcaattgc gtttgcggtt ggcaaaatgg cgcagcagca
     1561 aggcgtggcg gtgaaaacct ctgccgaagc tttgcaacaa gccattgacg ataatttctg
     1621 gcaagccgaa taccgcgact accgccgtac ctccatctaa gcctgcgccc ggtagtgaag
     1681 gctaccgggc aattttcctc tccctttttc agatctcatc catactgggt agtggcgaat
     1741 aaatctcatt tgcctcacct gctatgcaga acatcatccg aaaaggagga actatgaagg
     1801 ctgcagttgt tacgaaggat catcatgttg acgttacgga taaaacactg cgctcactga
     1861 aacatggcga agccctgctg aaaatggagt gttgtggtgt atgtcatacc gatcttcatg
     1921 ttaagaatgg cgattttggt gacaaaaccg acgtaattct gggccatgaa ggcatcggtg
     1981 tggtggcaga agtgggtcca ggtgtcacct cattaaaacc aggcgatcgt gccagcgtgg
     2041 cgtggttcta cgaaggatgc ggtcattgcg aatactgtaa cagtggtaac gaaacgctct
     2101 gccgttcagt taaaaatgcc ggatacagcg ttgatggcgg aagagtgcat cgtggtcgcc
     2161 gattacgcgg taaaagtgcc agatggtctg gactcggcgg cggccagcag cattacctgt
     2221 gcgggggtca ccacctacaa agccgttaag ctgtcaaaaa ttcgtcccgg gcagtggatt
     2281 gctatctacg gtcttggcgg tctgggtaac ctcgccctgc aatacgcaaa gaatgtcttt
     2341 aacgccaaag tgatcgccat tgatgtcaat gatgagcagt taaaactggc aaccgaaatg
     2401 ggtgcagatt tagcgattaa ctcacgcacc gaagacgccg ccaaaattgt gcaggagaaa
     2461 accggtggcg ctcactctgc ggtggtaaca gcagtagcta aagctgcgtt taactcggca
     2521 gttgatgctg tccgtgcagg cggtcgtgtt gtggctgtcg gtctgccgcc ggagtctatg
     2581 agcctggata tcccacgtct tgtgctggat ggcattgaag tggtcggttc gctggtcggc
     2641 acgcgccagg atctaaccga agccttccag tttgccgccg aaggcaaagt ggtgccgaaa
     2701 gtcgccctgc gtccgttagc ggacatcaac accatcttta ccgagatgga agaaggcaaa
     2761 atccgtggcc gtatggtgat tgatttccgt cactaagagg tctttgctgc gactcccatg
     2821 ttcgggtcgc agcatcgcac actctccaac atgaaatggc taaatggatt attgttgcgt
     2881 aggaaggaac aataatgata gtgttcccag agaaggaacg ctactgagag taaagacact
     2941 aatgaaaatg gccaatcatc cccgcccggg ggacattatt caggaatcac tggacgaact
     3001 taatgtcagc ctgcgcgagt ttgccagagc aatggaaatt gcgccctcaa cggcaagccg
     3061 attgctgacc ggaaaagcag ctttgacgcc agaaatggca attaaacttt ccgtggtgat
     3121 cggcagttcg ccgcaaatgt ggctgaatct gcaaaacgcc tggagtctgg cagaggcaga
     3181 aaaaacggtg gatgtgtcgc gattacgccg tctggttacg caataaaaaa tggcacctta
     3241 taaagcgcca tttgaaactg atgacaaacg caacattgcc tgatgcgcta cgcttatcag
     3301 gcctacacgt tcacttcaat ctattgtatt tgaagggttt tcatgccgga taaggcattc
     3361 acgacgcatc cgggatgaac aaagcccacg ttgtctacaa tatgaaaatg gcgcctttta
     3421 cagcgccatt ttcagcttaa agttcaaaag agattatatc ccttcttcac tctctttttt
     3481 cgcttctgcc ttctcgattt cacgatacca gcgcggatgg tgtttcttcg cccagcgacg
     3541 gctcaccttc ccttcgatca tccctttaat cgaccctttc acccagaatg ccatatacat
     3601 atggatcagg atggcgtgga tcaggatgat acccgcagcc gcgtggatca gcaggctgta
     3661 gcgaacaacc tgcatcggga agtactgcgc aaagtacgga cgccagataa tcaccccggt
     3721 caccagcagc acgaaaatca tgctcatgat cgaccagaac atcatctttt gcccggcgtt
     3781 gtacttacca acatccgcca ctttgtgctc attgcctttt aatacttcga caatgttcaa
     3841 cagccacgga atatctttct tatccgggat gttgtgatgc acaaaacgca caaacataaa
     3901 catcagtgcg acgaaaatcg caatgccgaa gaacgggtgc aaaatgcgtc ccatctgcgg
     3961 cgtaccgaag gtttgcgtca gccattgcag cgtcgggaag aagaacgaaa tcccggacag
     4021 cgccaccagg aagaagcaaa tcaccacggt ccagtgacag gcgcgatcaa taaatttggt
     4081 gcgcacaatc attttcgact tactcatgat gatcctcctc gtcatcgtcc acttccttat
     4141 tcgggccaat accgatgtag tggaaaatca acccggcaaa agtggcaata aagccagccg
     4201 ctgccagcgg tttcaacgcg cctttccaca ggcttaccga ggtgtcgatc ttcggatctt
     4261 tcggcagacc gtaatacagc tccggctgat cggcgtgatg cagcacgtac ataacgtgcg
     4321 taccaccgac ccctttcggg ttgtagacgc cagcatgttc gtaaccacgc gctttcagtt
     4381 tcgccacgcg ctcttccgcc agctccagca tctccttctt ggtgccgaag tggatagccc
     4441 cggtcggaca ggttttcaca caagccggtt cctggccgac gctgacgcga tcgacgcaga
     4501 gcgtgcattt atatacccgg ttatcctctt tgttgaggcg cggaatatta aacggacacc
     4561 cggcaatgca gtaaccacag ccgatgcagt tttccgactg gaaatcgaca atcccgttag
     4621 cgtactgaat gattgcacca gcagacgggc acgccttcag gcagccggga tcttcacagt
     4681 gcatacagcc gtctttacgg atcagccact ccagcttgcc gttctgttcg gtttcgctaa
     4741 agcgcatcac cgtccaggac ttggcgctca gatcggcggg gttatcgtaa accccgacgc
     4801 agtgccccac ttcatcacgg atgtcgttcc actccgaaca cgccacctga caggctttac
     4861 agccgataca ggtggaaacg tcgataagtt ttgcgacttc tgctttgtaa tcacgcacct
     4921 gagaaggcgg cgtgatggag ttagttgcgg accttttgat aatatcctgc gtttccatag
     4981 ccatctgttc gcccccttac gccttctcga tgttgactaa gaacgcttta tattccggcg
     5041 tttgcgagtt tgcatcaccg acattcggcg tcagagtgtt agcgatataa cctttacgcg
     5101 cgacaccctc aaagccccag tggattggaa tacccaccgt ttcaacctgc tgaccgttta
     5161 cattcagcgg tttcagacgg cgcgttacca ccgccaccgc gcggataaag ccacgcttgc
     5221 ttgagacagt gacacgatcg ccattattaa tgcctttcgc cgccgccagc gtttcgctga
     5281 tttccacaaa ctgttccggc tgagcaattg cgttgagcaa tgcgtgcttg gtccaggtgt
     5341 ggaagtgctc ggtcagacga taggtcgtac ccacatacgg gaactgctct tttttaccca
     5401 tccgcagcgc gtcttgttca tacagacgaa caaccgggtt agacaccacg ttcggatgca
     5461 gcgggttagt gcccagcggc gtttcaatcg gctcgtagtg ttccgggaac ggaccttccg
     5521 ccattttgtt gatggcaaac aggcgtccca tcccttccgg ctgcatgata aacggcccgg
     5581 ttggcgtacc cggtgcggca ttgccgaagt ccggaatatc gttacccgtc cacttgctgc
     5641 cgttccactg gatcagcatc cgtttcggat cccacggttt accattgata tccgccgaag
     5701 cacggttgta gagcacgcga cggttgagcg gccacgccca ggcccatccc agcgtattcc
     5761 ccagaccgga tgggtcggag ttatcgcggt tagccatctg gttgccctgc tctgtccagc
     5821 taccggtgta gatccagcaa gaagatgcgg ttgtaccgtc atcacgcaga tgcgcaaagc
     5881 tactcagcaa ctgacctttc ttcgcaatca gcacgccatt agcgtcatag agatcttcca
     5941 gcgcatagcc gttattctct ttcgccactt cgtcagattg cggttcgtgc ggctgcttgt
     6001 agttccagct catcttcatc agcggttcta cgcctttacc accttcggcc tggtacagct
     6061 cgcgcagatg atggtagata cccgccagaa tttcgccgtc gttacgcgct tcgcccggcg
     6121 cgtcctgacc tttccagtgc cactgcaacc agcgaccgga gttagcgata gaaccatctt
     6181 cttcggcaaa gcaggtcgaa ggcagacgga atacttcagt ctgaatagac gccggatcga
     6241 catcgttcga ctcgccgtgg ttctgccaga aggtagaggt ttcagtcacc agcggatcga
     6301 taaccaccat gtacttcagc ttgctcaggc agctcaccac tttgtttttg tccgggaagg
     6361 acgcaaccgg gttaaagccc tggcagaagt aaccggtgac tttgccttcg tccatcatgt
     6421 tgaaatactt gatgacgtcg taggtctggt cccacttcgg cagccagtca tagccccagt
     6481 tgttctcttt ctgcgcggca tcgccataga aagatttcat caggctaacg aagaacttcg
     6541 gatagttgct ccagtagttc acctgatcag ccagcgtcgc tttcggcgtg ttcgcttcca
     6601 gatacgactg caaatcaacc tgtttttctg acggaagcgt cagataacct ggcaggctgg
     6661 tagagagcag acctaagtca gtcagcccct gaatgttgga gtgaccacgc aatgcgttca
     6721 cgccgccacc ggccataccc atgttaccga gcagcaactg gatcatcgcc atagtacgga
     6781 tgttctgcgc acccacggtg tgctgcgtcc agcccagcgc gtacaggaag gtggttgtgc
     6841 gatccggtgc gctggtggag gccagcactt cacacacttt caggaagtcg gcttttggcg
     6901 taccgcagat gttttcaacg acgtccggcg tgtagcggga aacgtgcgct ttcagcaggt
     6961 tccacacaca gcgcggatga gtcagtgttt catcgcgttt tgcatagccg ttttcatcga
     7021 actgatagtt ccaggacgac ttatcatact ggcgtttttc agcgtcgtag ccgctgaaca
     7081 ggccgtcttc gaaagcaaaa tcatcacgca ccagcaggct ggcgttggtg taatgtttaa
     7141 cgtattcggc gttgattttg ttgttttcga tcaggtagcg caaaacgcca gacaggaacg
     7201 taatgtccgt accggaacga ataggtgcgt aaatatccgc cacagaagcg gtacgcgtaa
     7261 aacgaggatc gacaacgatc aaggttgcat cgttgttgtt tttcgcttcc atcgcccagc
     7321 ggaaaccgac gggatgcgct tcagcagcgt taccgcccat taccattacg acgttagcgt
     7381 ttttgatatc cacccagtgg ttggtcatcg caccgcgacc aaatgttgga gcaagacttg
     7441 ctaccgttgg tccgtgtcag acgcgcgcct ggttgtctac cgccagcatc ccgagggagc
     7501 gggcaaattt ctgggtcagc atcccggttt cgttgctggc accggaggca cacagcatac
     7561 cggtagaaag ccaacggttt accgttacgc cctgctcgtt cttttcaata aagttagcgt
     7621 cacggtcagc tttcatcagc ttcgcaatac gggagaatgc ttcttcccag ctaatgcgct
     7681 gccatttgtc agaacctggc gcacgatatt ccgggtagcg cagacggttt tcgctgttga
     7741 cgtaatccag caaaccggcc ccttttgggc acagcgcacc acggcttacc ggatgatccg
     7801 ggtcaccttc aatgtgataa atcgcttctc tggcgttttt cgcgccatca cccaggctat
     7861 acatcaatag cccgcaacct acggaacagt atgtgcaggt gttacggatc tctttagcgc
     7921 gtaataattt gtagtttcgc gcctgagcca gtgcttgctt cggggcaaag cccaatgccg
     7981 ctaccgttgt tccagccata ccgcccgcgc agattttaaa aaattgtctg cgactgacgt
     8041 ccattgcttt cctctttttt caggggtatt actgcgcgag gaaactaaca gtaaaattct
     8101 gaatcttttt gcgcgaaatc aaaaagaaca cgtgtatgcc acaaaataat ccgcctgcgc
     8161 aaattaacct acctcaatag cggtagaaaa acgcaccact gcctgacagg ccagttaaaa
     8221 aaatgctata aaattcagct taatttttaa cggcaagaga gacaaaacag cgagcatgac
     8281 acgacaaaaa gcaacgctca tagggctaat agcgatcgtc ctgtggagca cgatggtagg
     8341 attgattcgc ggtgtcagcg aggggctcgg tccggtcggc ggcgcagctg ctatctattc
     8401 attaagcggg ctgctgttaa tcttcacggt tggatttccg cgtattcggc agattccgaa
     8461 aggctattta ctcgccggga gtctgttatt cgtcagctat gaaatctgtc tggcgctttc
     8521 cttagggtat gcggcgaccc gtcatcaggc gattgaagtg ggtatggtga actatctgtg
     8581 gcccagcctg acaattctct ttgccattct gtttaatggt cagaaaacca actggctgat
     8641 tgtaccagga ttattattag ccctggtcgg cgtctgctgg gtgttaggcg gtgagaatgg
     8701 gttacattat gatgaaatca tcaataatat caccaccagc ccattgagtt atttcctggc
     8761 gtttattggt gcgtttatct gggcagccta ttgcacagta acgaataaat acgcacgcgg
     8821 atttaacgga attaccgttt ttgtcctgct aacgggagca tgtctgtgga tttactattt
     8881 tctcacgcca caaccagaaa tggtatttag cacgcccgtc atgattaaac tcatctctgc
     8941 ggcatttacc ttaggatttg cttatgctgc atggaatgtc ggtatattgc atggcaatgt
     9001 caccataatg gcggtaggct cgtattttac gcctgtactt tcctcagcgc ttgcagccgt
     9061 gctgctcagc gccccgctgt cgttctcgtt ctggcaaggc gcgctaatgg tctgcggcgg
     9121 ttccctgctc tgctggctgg cgacacgtcg tggttaaaat aattgccggg tctacggccc
     9181 ggcagttaat cagataaatt ccgacaatat taaaaaataa atttcaaata ccagtaattc
     9241 accttatatc tcatttcgac ttcgatcaca tatcgataaa taatattatt tcatctattt
     9301 ttctcattgt ttttaatcac cattctattt taaaaacagt atgtcattta taaatgacgc
     9361 cgtttaaaat tcgtttagaa aatacgttta acgataatta aaaggattct gttaaatgaa
     9421 attaaaaata gttgcggtgg ttgtaactgg tttgttagct gcgaacgtag cacacgctgc
     9481 cgaagtctat aacaaggatg gtaataaact cgacctttat ggcaaggtta ccgctctacg
     9541 ttattttact gatgataagc gtgacgatgg tgataaaact tatgcccgtc tcggctttaa
     9601 aggagaaacg caaatcaatg atcaaatgat tggttttggt cactgggaat atgattttaa
     9661 aggctataac gatgaagcca acggctcgcg cggcaacaag acccgtctgg cctatgctgg
     9721 tttaaaaatt agtgaatttg gctctctgga ctatggtcgt aactacggtg tcggctacga
     9781 cattggttca tggaccgata tgttgccaga atttggtggc gatacctgga gtcagaaaga
     9841 tgtcttcatg acataccgta ccaccggtct agcaacctat cgcaactacg atttctttgg
     9901 cttaattgaa gggctgaact ttgccgcgca atatcaaggc aaaaatgaac gtactgacaa
     9961 cagtcatctt tatggtgctg actacacgcg tgccaacggt gacggtttcg gtatctcctc
    10021 aacttatgtt tatgatggct ttggtatcgg tgcggtatat accaaatccg atcggacaaa
    10081 tgcgcaggaa agagccgctg ctaatcctct caatgcctcc ggtaagaatg cagaactatg
    10141 ggccacaggt ataaagtatg atgccaacaa catctacttt gcagctaatt acgctgaaac
    10201 attaaacatg accacctatg gggatggtta tatctctaac aaagcacaaa gttttgaagt
    10261 ggtggcgcaa tatcaattcg acttcggctt gcgcccatca ctcgcttacc tgaaatcgaa
    10321 aggcagagat ctgggccgct acggcgacca ggacatgatt gagtatatcg acgttggtgc
    10381 gacgtatttc ttcaacaaaa atatgtcgac ctatgttgat tataaaatca acctgattga
    10441 tgaaagcgac tttacccgtg ccgtagatat tcggaccgat aacatcgtcg ctacgggcat
    10501 tacctatcag ttctaacgtc ctctcctgac cagcccttct ctgggctggt ttttgattta
    10561 agtgctgctg tatgactcct taagtaaaaa tgagcaattt ttgatatttc tggtcattca
    10621 cctcaattaa tattattcca tatcctccgg ggacatctta ctataatatt tattgatgag
    10681 tgattatgac caatattaat acagcttgtg taaaaaataa tgccagttat caacttaata
    10741 acgcattacc taacaaagaa accatctcca gcaatttttg tgaacgacta gaacaatggg
    10801 gtaataaatc ggttaataat ggtgaagaaa gagcaattgc cgtggagcga attaaagaag
    10861 cttacaattc gaacatggca tctcttgatt tgtcatatct tgatttgagt gaattaccgc
    10921 ctatcccctc gacagttaat acgttaaatc tggaaaacaa ttgtctaact tgtcttgact
    10981 tagctgacaa tgccagtctc gttaatatca acctcagcgt taataaaatt aacacgataa
    11041 cctttccaaa agaatcaaaa ctggaaaaca tttatattga tcataacaat ctggaaagtt
    11101 tagatttaaa aaatcagcat tcattggtta atctggaagc gcaaaataac aaactgacaa
    11161 aaattaatat ttctgatagt tataaactga aatttcttaa tcttgattat aacaagctag
    11221 catcgctgga tgtctcccgg caagaatctc tgattgagtt aagtgcccat cacaatatga
    11281 tcactgacct tatattacac aatcatccca ggatgaaaac aatcacttta aacgacaacc
    11341 atattgcaca tttaaacgcc aaaaacacta caaaactgga atatttaaac ttaagcaata
    11401 acaatttact gccaaccaat gacattgatc aactaatatc atcaaaacat ctttggcatg
    11461 tattagttaa cggcatcaac aatgatccac ttgcacaaat gcagtactgg actgcagtaa
    11521 gaaatataat tgatgacact aatgaagtga ccattgattt atcaggactt aatttaacca
    11581 ctcaaccacc agggctgcaa aacttcacat ctatcaatct tgataataac caactcacac
    11641 attttgatgc aaccaactac gatagactcg taaagctaag tctgaatagt aatgctcttg
    11701 agtcaataaa ttttcctcaa ggcagaaatg taagtattac acatatatct atgaataata
    11761 atgctctcag aaatattgat atagataggc tttcatcagt tacttatttt agtgcggcac
    11821 ata
//
LOCUS       DAJVQG010000121        11777 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJVQG010000121 DAJVQG010000000
VERSION     DAJVQG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.121"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..609)
                     /locus_tag="OQM83_003024"
     CDS             complement(<1..609)
                     /locus_tag="OQM83_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCS1521227.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRR"
     gene            complement(732..2378)
                     /gene="fumB"
                     /locus_tag="OQM83_003025"
     CDS             complement(732..2378)
                     /gene="fumB"
                     /locus_tag="OQM83_003025"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCS1521228.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2456..3796)
                     /gene="dcuB"
                     /locus_tag="OQM83_003026"
     CDS             complement(2456..3796)
                     /gene="dcuB"
                     /locus_tag="OQM83_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCS1521229.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4367..5086)
                     /gene="dcuR"
                     /locus_tag="OQM83_003027"
     CDS             complement(4367..5086)
                     /gene="dcuR"
                     /locus_tag="OQM83_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCS1521230.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5083..6714)
                     /gene="dcuS"
                     /locus_tag="OQM83_003028"
     CDS             complement(5083..6714)
                     /gene="dcuS"
                     /locus_tag="OQM83_003028"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCS1521231.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6895..7125
                     /locus_tag="OQM83_003029"
     CDS             6895..7125
                     /locus_tag="OQM83_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HCS1521232.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7137..7409
                     /locus_tag="OQM83_003030"
     CDS             7137..7409
                     /locus_tag="OQM83_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1521233.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7636..7932
                     /gene="ghoS"
                     /locus_tag="OQM83_003031"
     CDS             7636..7932
                     /gene="ghoS"
                     /locus_tag="OQM83_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCS1521234.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7960..8133
                     /gene="ghoT"
                     /locus_tag="OQM83_003032"
     CDS             7960..8133
                     /gene="ghoT"
                     /locus_tag="OQM83_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HCS1521235.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(8252..9769)
                     /gene="lysS"
                     /locus_tag="OQM83_003033"
     CDS             complement(8252..9769)
                     /gene="lysS"
                     /locus_tag="OQM83_003033"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCS1521236.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(10006..11463)
                     /gene="dtpC"
                     /locus_tag="OQM83_003034"
     CDS             complement(10006..11463)
                     /gene="dtpC"
                     /locus_tag="OQM83_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HCS1521237.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(11522..>11777)
                     /locus_tag="OQM83_003035"
     CDS             complement(11522..>11777)
                     /locus_tag="OQM83_003035"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCS1521238.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGTHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
BASE COUNT         3042 a         2856 c         2891 g         2988 t
ORIGIN      
        1 gcgccgtagc tggcggcaat gaaagcgagc gaggaatatc accgtcgtta atgcgccaag
       61 cagcgcgcag aacatatcgg attgcgtgtc ccactggtcg ccctgggtgc cgagaaaatc
      121 atccgctccc tggcccattg ccagcgccgc ccaccactcg attaattcat acatcgcgct
      181 tatcgccagc gccacgcagc agactaggaa cgccaccatt ttacgtccgc gcacgtacat
      241 cccgcgcacg agaatttctc gtgccaccag tgcaggcacc agcccctgga aaaaatgccc
      301 cagcttgtca tacggattac ggctcaaccc cagccattcc tgcacctcaa aaccgatggg
      361 gactttcgcg taggtgtatt gcccgccgac catcaggatg atggcgtgaa agaaaatgag
      421 cgtatagagc agcggcgtta acggataacg tttagcggtg gcaagcagta gcggcacgac
      481 aataatcacc ggtgtcactt ccatcagcca ggtgagtttg tcatgggccg aaatgccggt
      541 ataaatcagg attaacgtta gcgccagtgc gccggtgtta agaattaacg gtttgagtgt
      601 gcgggtcatg gtgagttcgc aagtcaggga aaatactgac tattcaccag caagcatgaa
      661 attacaatcg caaaagaggt ggcccagggg gatcacctgg cagcatgctg ccaggcgctg
      721 ggccgaagag gttacttagt gcagttcgcg cactgtttgt tgacgatttg ctggaagaag
      781 tcgttacctt tgtcatcgac caggataaac gccgggaaat cttctacttc gattttccag
      841 atagcttcca tacccagctc cggataagcg acgcattcca gatgcttgat gctctgctgc
      901 gccagtaccg ccgccggacc gccgatgcta ccgaggtaga agccgccgtg tttatgacac
      961 gcgtcggtaa cctgctggct gcggttgcct ttcgccagca tgatcatgct gccgccgtgg
     1021 gattgcagca gatccacata ggagtccata cgacctgcgg tggttgggcc aagtgaacct
     1081 gatggataac cggcaggagt tttcgccgga cccgcgtagt agatcgggtg atctttgatg
     1141 tactgcggaa gttctttacc ggcgtcaatc agctctttca gcttggcgtg tgcaatatct
     1201 cgacccacaa taatggtgcc agtgagcgac aaacgagtgg aaaccggata ttgcgaaagc
     1261 tgggcgagga tctctttcat cgggcggtta aggtcgacct tcaccgcttc gccttcaccg
     1321 gcctgacgca gttcttgtgg aatgtactga cctggattgt gttccagttt ttcgatccag
     1381 ataccttcgc ggttgatttt cgctttaatg ttacggtcag cggagcagga gacgcccatg
     1441 cctaccgggc aggatgcgcc gtgacgtggc agacggataa cgcgaatgtc gtgcgcgaag
     1501 tatttaccgc caaactgcgc gccaagaccg agtttctggg cctcttccag cagttcctgt
     1561 tccagctgga catcgcggaa cgcctgacca tgttcgttcc cttccgtcgg cagttcatcg
     1621 taatagtgag cgcttgctaa tttgacggtt ttcaggttgg tttccgcaga cgtaccgcca
     1681 atcacaaacg cgatatggta cggcgggcag gctgcagtac cgagggtacg cattttctcg
     1741 acgaggaagt ttttcagttt gccgggagtc agcagggctt tggtttcctg gtagagatac
     1801 gttttgttgg cagagccgcc gcctttcgca acgcaaagga atttgtactc atcgccatct
     1861 accgcgtaca ggtcgatttg cgcaggcagg ttagtgccgg tgttgacctc tttgtacatg
     1921 tccagcgccg cgttttgcga gtagcgcagg ttatcttcga tataggtgtt atagacgcct
     1981 ttcgacagcg cttcttcatc accgccaccg gtccacacgc gctggccttt tttaccgacg
     2041 atgatcgcgg tgccggtatc ctggcaggtc ggcagcacgc ctttggcggc gatttcggag
     2101 tttcttaaga attgcagtgc cacgtacttg tcgttttcgc tggcttctgg atcgtgaaga
     2161 atagccgcaa cctgtttctg gtgtgccgga cggagcatga aagaagcgtc gtgaaaggct
     2221 tgctgcgcca gcagggtcag ggcttctggt tccactttca ggatggtttc gccgtcgaag
     2281 tcggcaacgc taacgtaatc ggaagtgagt agatagtatt cggtattgtc tttccccatc
     2341 gggaaaggtg cctggtagat aaagggtttg tttgacatag cttccagcct gtaactctgt
     2401 atttgttatt cgaaaatggc gtgccgcgta gtcacgggcg gcacgcaaag tgcatttata
     2461 agaacccgta catcgcggcg aagatccagc cgaagacgca cgatacgctc acaccaatca
     2521 accccggcag aataaagctg tggttgatga cgaagcgacc gatgtgggtg gtgccggaac
     2581 ggtcaaactg aatcgctgcc agatcgctcg gataagtcgg caggatgtaa taaccgtagc
     2641 aagccggtgc tgaagccacg atgtatgccg gatcaacgcc gatcgccagc gcgaccggaa
     2701 caatcgccgc cagcgccgca gcctgagagt ttacaaactt ggaaaccagc agcagaacaa
     2761 tggcataggc ccacggatac tctttcacca tttcacccag tacgccctga atttcagaca
     2821 tatgcgcacc gaacatggtt tctgccatcc atgcgatacc gtacaccgcc acgatggcga
     2881 tcataccgga acggaagact tcgttttttg agatagacgc gggattggtt ttggtcagga
     2941 taataatcag cgccccggtc agcagcataa acatctgaat aaccagtacc atcgacagcg
     3001 gtttgccgcc gaaggatgga cgcaggtccg aatcagcacc aagaagggcg actacagcga
     3061 ttgccccgag gaaaatccac attgccagcc agttgctttt cggcagtttt ttgtccagca
     3121 gcgtcgcggt atcaccgtaa acatactcac ggttttccgg tacggagatg aatttctgga
     3181 actcttcgtc tttatccaga tctttaccac ggaaccagct gaagataccg atcgccagga
     3241 taccgattaa cgtcgatgga atggtgattg ccagcaggtc gaggaactca agatggcgac
     3301 catcaaaggt gacattaccc agcattgcaa ccagagacac gaccgcaacc gacaccggac
     3361 tggcgatgat ccccatctgt gcaccgatag aacttgccgc catcggacgt tccggacgga
     3421 tgttgttctt aatggcgacg tcgtagatga tcggcagaat ggtgtaaacc acatgacccg
     3481 taccgcaaag aatggtcagc gtacaggtca caaacggcgc gacaattgag acatatttcg
     3541 ggttgcggcg cagcagcttc tcggcaattt gcagcatgac atcaagaccg cccgaagctt
     3601 gcaaggtcgc cgatgccgcc accaccgcaa tgataaccag catgacatca accggtggtt
     3661 tacctggctg aaggtggaag acgaagacca gaatgaccag accgataccg cctaataaac
     3721 ccagcgcgat accacccttt ctggcaccat aaaacagaca tatcagtatt atgataagtt
     3781 ggatagtaaa taacatgtgt gaaccctcgc gataatccta tttaaatttt tgctgaatag
     3841 atcacagtca cgttctgttt tgtatgaact gttttcagag cctgactaaa tatccgtttg
     3901 gtcgttaccg gctttagcaa atacctcaca gtgaatattg gctgattaaa gcagcggcaa
     3961 atttatgcac tgtgataagc ggctttttca gagagaaaga ctctctggcg atgggtttat
     4021 agtttgtact gcgcagatgt taatgttttg tttgctgtta tcttattgat atttatcggt
     4081 ctattttttg ttctcagggc agatgtcatt aggtttatag attaatctga tctacccatt
     4141 tgtgggtaaa aatacacata atgcgggtga cataatagtt aattaacttt tgttagcgtt
     4201 ttgaaattaa aaacaccgtt cacctgaaga gatattaatt tttagcgatg atgcagggat
     4261 aattatattt gatctggcac aagttttact gatgaaggat gtaacttgtg ccaggggtat
     4321 tttgcattac ggtaataatt attacttaca gataaccagc gatccgttat tggcaatatt
     4381 gtttcagtag tgagtagtgt tctgcctgaa tacggtaacg gtaaactgga cgccccgtga
     4441 cgccataatg gatactggtg aacaagatgt ggcagttgac cagccagatg aggtatttac
     4501 ggcaggaaac acgcgaaatg ttaacctcgt tggctagctc gtcggttgaa aattcatagt
     4561 cctgatgcgc gtcaatccac tggcacagtg tgcgtaacgt ctgcggcgtt aagccttttg
     4621 gcaagcgacg aggatcctgt tcgttggagc tgctgccgtg gattagctga tcaagctcgg
     4681 cctggtcata atactgatgt ttttccagcg ccattttctt ttgccgccag tcggtgagcg
     4741 cctcttcaaa gcgggaagcc tggaagggtt tgatcaggta atccacgaca ccgtaatgca
     4801 gcgaatcttt aatggttgcc gcatcggctg cggaggagat gacaatcaca tcacttttgc
     4861 aacgcgcgtt atgcaggaca ggcagtaaat cgagcccgtt ctctttttgc atatatatat
     4921 cgagcaatat caggtcgata ggcgtatcgc tattgaagat aatctctttg gctttctcca
     4981 gcgtcgaggc tgttccacag cattgaaagc ctgggatttg tgctacgtat cggcgattca
     5041 gctccgcgac cattgcgtcg tcatcgataa ttaatacatt gatcatctgt tcgacctctc
     5101 cccgtcccag ggtatctgga caaaaaattg tgtgaaaatc ccgggttccg attccacggc
     5161 gatgctgccg ccgagatttt ctacctgttg tttgacaagt gctaaaccga cgcctcgctc
     5221 gcttcctttt gtcgagacac ctttgtcaaa aatgtgatcg attttgtcgg gtgcgatccc
     5281 cggtccatca tcgttaactt cgcagtgcag ccagccgtga cggtagtgca atgttacgct
     5341 aatttcgcca ccgggttccg gccctaatgc ttccagcgcg ttttctatca gatttcccaa
     5401 tgtggtaatc agcgtcgcga cctggtcctc actgccgctg tctggcagct ggctttcact
     5461 gtttaaaatc agcgtatggc ctaaatcggt cgcgcgatta atcttgctga ttaaaaaacc
     5521 agcgataacc ggagatttga tcttacccag cagagagcca atctcttcct gatagttatt
     5581 ggctgttttt agaatgtaat cttccaactg cttataactc ttcagatgca ataatccgag
     5641 aatcacgtgc aatttattca taaattcgtg ggatcgttca cgaagtgcgt cggcatagtt
     5701 gaccagaccg tcgagtcgct gcatcagttt acgtacttcg gttttgtccc tgaaggttga
     5761 aatggcaccg atgataacgc cattactgcg caccggaacg gtgttgatca gtaatagccg
     5821 gtctttaatc gtaatctctt cgtcgcggcg cggcgtaccg tcgcgtaaca cttccgagac
     5881 atctaccacc tgtgaccatg agtggctgag cgtcgacagt ttctcatcgt cctgcgactt
     5941 acggtaattc agcaattctt gtgcggcatc gttgatcagc gtgacctcgc cgcgatcgtc
     6001 cacggcaacg acgccttctt tgatagactg caacatggcc tggcgttgct caaacagcgt
     6061 ggagatttcg tagggttcca ggccgaaaag gatttttttc agtaccttaa ccagaatgca
     6121 ggtgccaatc agtccgacca gcatgccaaa taataccgac cagataatgc tccagcgact
     6181 gtcattgatt tgttgggtca cgcggcttaa ctcaaggccg atcgccacca cgccaatttg
     6241 tttatgattt tcatcgtaga tgggggtaaa tacgcgtaaa gcctgcgcca gaaaaccgcg
     6301 attgatagcg acattttctt tgccattcag cgctttaagg atgtcatcac ctttaaatgg
     6361 ctgaccaata cgctgggctt caggatgcga gtagcgaaga ctttgcatat cggtaacgac
     6421 aataaacagc agatcgttgc gtttgcgcac ggcttccgcg atggcctgga tgccgctctc
     6481 ctgcggtttt ttctgcaagc cctgacggat ttccggcgag ttggcgaggg tacgcgccac
     6541 tgccagtgcc ttgttggcta gcccgtctcg cgtcatatca ctgatttgcg agaagtaaat
     6601 cagatgcacc accaatagca ccgagaacag taccgcactg accattaaga tcactgtggt
     6661 actcaatttc atcggacgtt tgcgtaacat gcggtagggc aatgaatgtc tcatcagctt
     6721 ccttgtgtga caaatttctt aaacattatc tctgatgagg tgggtaattc aaagggagta
     6781 agaatgattg gctatatagg ggaagagatt ctggcaacgg aaactgccag tgctgtatga
     6841 agattccagg gctatgctta tagcgataat catactgatg agagagggaa agtcatggat
     6901 caggcgctac tggacgaggg ttatcgctgt tataccggcg aaaagatcga tgtctatttc
     6961 aacactgcga tatgtcagca ttctggcaat tgcgtacgtg gcaacggcaa gttatttaat
     7021 ctcaaacgaa agccgtggat catgccggat gaagtcgacg tcgccactgt ggttaaagtg
     7081 attgatacgt gcccgagcgg cgcgctgaaa taccgtcata aataagcgag ggtaaaatgg
     7141 aaatacgcga aggccacaat aaattttaca ttaatgacga acaaggcaag caaatcgctg
     7201 aaattgtctt tgtgccgact ggagagaatt tagcgattat cgaacatacc gatgtcgatg
     7261 aaagcctgaa agggcagggg attggtaaac agctggttgc aaaagtcgtg gaaaaaatgc
     7321 gtcgggaaaa acgaaaaatt atcccactat gcccatttgc gaaacacgaa tttgataaaa
     7381 cgcgggagta tgatgatatt cgcagttgat gggagagtac agagtcacga tatttttcat
     7441 gctctccgcg gagtgatgca ggagagcatc tgaagggtag ggggatgcac aaagaatggg
     7501 cagagagcgc gtttttttgt cccaagtcat cccctttact gagcaaaaaa aagaatatct
     7561 cctatatgag aatcatcaat cggggttatc aagttttgcg tccccagagc attcaatatt
     7621 gataggagtc atattatgga aggtaaaaac aagttcaata cttatgttgt ttcttttgat
     7681 tatccatcat cttattcctc agtgttctta aggttaagat cattgatgta tgatatgaat
     7741 ttctcttcta tcgtggctga tgaatatggg ataccacgac aattgaatga aaattccttc
     7801 gcaataacga catcgttagc cgcaagtgaa atcgaagatt taatcaggct taaatgctta
     7861 gacttaccgg atattgattt tgacctcaac attatgacag ttgatgacta tttccgtcag
     7921 ttttacaagt agccaggaag gataagaaga acataaaaaa tggcactatt ctctaaaata
     7981 ttaatttttt atgtgattgg tgtgaacata tcctttgtca ttatctggtt tatctcacat
     8041 gagaaaacac atattcgttt acttagtgca ttcctggtcg gaataacctg gccaatgagt
     8101 ctgcctgtgg cattactttt ttctctcttt taggagctgt agttgagtgg acccgctggt
     8161 tatcgtgaaa cgaagcgtct ctcctcaaaa ctggatttgc ggaacgacga acgaagcagt
     8221 cgcctgactg cttcgttcat taaagtgaaa tttatttctg tgggcgcatc gccgggaaga
     8281 gaataacgtc gcgaatagta tgactgtttg taaacagcat aatcattcgg tcgataccaa
     8341 tacccagacc agcggttggc ggcagaccat attccagcgc agttacgtaa tcttcgtcat
     8401 agaacatggc ttcgtcgtca cctgcagctt tagcgttaac ctgttcctgg aaacgttcag
     8461 cctgatcttc tgcgtcgttt aattcgctaa aaccattacc gatttcacga ccaccgatga
     8521 agaattcaaa acggtcggtg atttccgggt taacatcatt acggcgcgcc agcggtgaca
     8581 cttctgccgg atattccgta ataaaggttg gctgaatcag atgtgcttct gccacttcat
     8641 caaagatctc cgtgataata cgtcccagcc cccagctttt ctctaccgta attccgatag
     8701 attcagctaa tgctttagca gcatcaaaat tatccaggtc ggccatatcg gtttctggac
     8761 gatatttttt gattgcttcg cgcatggtga gtttttcaaa cggtttgccg aaatcaaaca
     8821 catgctcgcc ataagtgact ttagtggtac ccagaacctc ttgtgccagg gtgcggaaca
     8881 gtgactctgt cagttcaatc aaatcgtggt aatccgcata cgccatgtag agttccatca
     8941 ttgtgaactc aggattatgg cgaacagaaa taccttcatt acggaagtta cggttgattt
     9001 cgaatacccg ttcaaaaccg cctacaacca gacgtttcag atacagctcc ggcgcgatac
     9061 gcagatacat atctaaatcc agagcattat gatgggtaat aaacgggcga gcagatgccc
     9121 cacctggaat tacctgcatc atcggggttt ctacttccat aaagccgcgc gcgaccatga
     9181 actgacggat agcggccaga atttttgaac ggacaacaaa cgtttgacgg gatttatcgt
     9241 tagcgatgag gtccagataa cgttgacgat aacggacttc ctgatcctgc agaccatgga
     9301 atttatctgg taaaggacgt agtgctttag tcagcaggcg caactcagta cagtgaatgg
     9361 aaagctcacc cgtttgcgtc ttaaacagcg taccgcgggc accgataatg tcacccagat
     9421 cccatttttt aaactgatcg ttataaacac cttctggcag gctatctctt gcaacgtaca
     9481 gttgaatacg gccaccgaca tcctgcaacg ttacaaagga ggctttcccc atgatacgac
     9541 gggtcatcat tcggccagca accgagactt caatgtttaa ggattccagt tcctggttat
     9601 tcttcgcatc aaactcttcg tgcaactggt cagaggtatg gtcgcggcga aaatcattgg
     9661 gaaacgccac accttgctga cgtagtgccg ccagtttttc gcggcgattt ctcagttcat
     9721 cgttaaaatc aatagcctca ttggctcccc gtgtttcttg ttcagacatt ttggttcctc
     9781 taaatccagc tttcaattta gcgtagataa agagacagat cggtacactg tattatctgc
     9841 ctcgactatt aataactcag tttatcttat cgaaactaac gcttacctca agttgatatc
     9901 actaataaat aaccatcaaa atggtttaaa tgacttatta atctcgattt actagtgtga
     9961 ttaactgaaa gttaatttat tcataaccaa ttgtttttac gacagttaat cgttgctctc
    10021 ctgtatcata ttcgtcggcg tgctaaacag aaaacgggta gcaaaggcca atacgacgat
    10081 gatcgcgaca caagccaacg tccactctcc catctgagaa aagaatcgct ggtaagctgc
    10141 aattaccata ccgctaattt gcgactctgt cgtctgctgt gccacgacgc ctgccagcca
    10201 gttggcgacc gcgcctgttg ccagcatata aataccggtt aatacgccag acattttcag
    10261 acgcgtgatt tgcgcaatcg ccaccgggtc aataaagagt tctgcaaagc ccattagcgc
    10321 caacccggat atcatcacgc ccattgacgc ttgaccgtca gccgctgcat gtcgggcatc
    10381 aaatgccaac aacataaagc cacaagccat cagcagtaag ccaaaggcaa acttcagcca
    10441 gacgcgcaat gttgagttgc cgcggctttc tggcgacgcc agccaggcca gtacaacccc
    10501 agcgagcatc actgcaattg cattcaccga ctggaatagt gctgtaggta cttcaatatt
    10561 gaatgcctga cgattcacaa agcgatcgat aaacaagctg atggtactgc cgccctgttg
    10621 tgccagtacc cagaacaacg tcccgacaaa catcaacaat acaatttgcc aaagagcacg
    10681 gcggtgttcg gggaatttga tcatcatgcg ggcaatgatt tgtgcggcaa tgaggcaaac
    10741 gatcgccagc aaatatccgg accagtcgtt ctccagcagc agggtaaaaa atactggggc
    10801 taaacagagc atcaccacta accagctcca tactggtaag gcaaatttga cgctagtgag
    10861 cgctttttta tccatactac gcgtggactg gaaatgacga tgaccgctta agaaaatcaa
    10921 caaaccgata aacatgccgc cacccgcaag ggcaaagcca acatgccatc cataccactg
    10981 agcagccagg ccgcaggcga ttggggcggc gatagaaccg atattgcccg cagcatacag
    11041 cagcgaaaaa ccgccatcac gtcgatgatc gttctcgtcg tagagctcgc caagcaaaca
    11101 gctgatgttt gatttgaata aaccgtagcc acaaataatg attgccagcg ccagatacag
    11161 gctaaaggtt gaatttgtat caatgcccag caccacatgg ccaagggtca ttaacagcgc
    11221 gccggcaatc actgcagtgc ggttgccgag caggcggtcg gcaagccagc cgccgagaat
    11281 aggggtaacg taaaccagag aagcatatgc gctgaacagg ctgatggcat ggttatcatc
    11341 aaaaccaagc tgatgggtga gatagagaat gagtaaggca cgcatgccgt aaaaactgaa
    11401 gtactcccag atttggatcg ccacgatata gtatatcgcg cgcggctgtg agggtgtttt
    11461 catgtgttct ccttatgagc aaaaaaggga agtggcaagc cacttccctt gtacgagcta
    11521 attatttttt gctttcttct ttcaatacct taacggtata gcggccatca gcctgacggt
    11581 atgcaccgtg aatatcggtt tcaaatcccg gatagtgagt gccgatttca cacagcatct
    11641 gcaggaactc cagaaccgga cggctttctt cggtgatcat ttcacccggc attaccagag
    11701 gaactcccgg cggatacgga aggatcatat tggcgttaat acgacctacc atttcgtcga
    11761 ggtaaacttc ttcggtc
//
LOCUS       DAJVQG010000122        11652 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJVQG010000122 DAJVQG010000000
VERSION     DAJVQG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11652
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.122"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..488
                     /locus_tag="OQM83_003036"
     CDS             <1..488
                     /locus_tag="OQM83_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1521239.1"
                     /translation="NVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHH
                     RKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRR
                     NPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARL
                     S"
     gene            540..1475
                     /gene="ttcA"
                     /locus_tag="OQM83_003037"
     CDS             540..1475
                     /gene="ttcA"
                     /locus_tag="OQM83_003037"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCS1521240.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="OQM83_003038"
     CDS             complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="OQM83_003038"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCS1521241.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="OQM83_003039"
     CDS             complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="OQM83_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCS1521242.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            4693..5925
                     /gene="dgcM"
                     /locus_tag="OQM83_003040"
     CDS             4693..5925
                     /gene="dgcM"
                     /locus_tag="OQM83_003040"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HCS1521243.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5946..6509)
                     /gene="smrA"
                     /locus_tag="OQM83_003041"
     CDS             complement(5946..6509)
                     /gene="smrA"
                     /locus_tag="OQM83_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCS1521244.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6839..7747)
                     /locus_tag="OQM83_003042"
     CDS             complement(6839..7747)
                     /locus_tag="OQM83_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1521245.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7923..9233
                     /gene="abgA"
                     /locus_tag="OQM83_003043"
     CDS             7923..9233
                     /gene="abgA"
                     /locus_tag="OQM83_003043"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HCS1521246.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAVTASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9233..10678
                     /gene="abgB"
                     /locus_tag="OQM83_003044"
     CDS             9233..10678
                     /gene="abgB"
                     /locus_tag="OQM83_003044"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HCS1521247.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            10715..>11652
                     /locus_tag="OQM83_003045"
     CDS             10715..>11652
                     /locus_tag="OQM83_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCS1521248.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMLPMGALIFLAVD
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFF"
BASE COUNT         2972 a         2962 c         2887 g         2831 t
ORIGIN      
        1 tgaatgtcag aagaaacctg acgatacttg atatgttcgg tcccccaaaa acaaatgccg
       61 ggatccggac ggtaacatta ctgcagccgg ctcttgaagc actgaaggag caatacaaac
      121 tgaccgggca tcatcgcaaa agcgaaatca ctttttatca tcgggagtac ggcagaactg
      181 aaaagcaaaa actgcatttt gttttcatgc ccagggtgtg taacgaaaaa cagaaacctt
      241 attactcggt aagcagtttg ggtgcaagat ggaatgcagc agtaaaacgt gctggtattc
      301 gccgccgtaa tccgtaccat acgcgacata cttttgcctg ctggctgttg acggcaggag
      361 cgaacccggc atttatagcc agccagatgg ggcatgaaac tgcgcaaatg gtgtatgaaa
      421 tttacggtat gtggattgat gacatgaacg acgaacagat agccatgttg aatgcgcggt
      481 tatcgtagtt gcaaagtttg cccccaattt gccccattta gtaccagaga actgaaataa
      541 tgcaagaaaa tcaacaaatt acaaagaaag aacaatacaa cctgaacaaa ttacaaaaac
      601 gtctgcgtcg taacgtgggc gaagccattg ctgacttcaa tatgattgaa gaaggcgatc
      661 gcatcatggt ttgcctctcc gggggtaaag acagctatac catgctggag attctgcgca
      721 atttgcagca aagcgcgcca atcaattttt cgctggtggc tgttaacctc gatcaaaagc
      781 aaccgggctt cccggaacac gttctgcccg agtatcttga aaagctgggc gttgagtaca
      841 agattgtcga agagaacact tacggtatcg tgaaagagaa gattccagag ggcaaaacca
      901 cttgctcact gtgttctcgc cttcgtcgcg gtatccttta tcgtaccgca acggaactgg
      961 gggcgacgaa gatcgcgctg ggtcaccatc gtgacgatat cctgcagacg ctgttcttaa
     1021 atatgttcta cggcggcaag atgaaaggta tgcctccgaa actgatgagc gatgatggca
     1081 aacatatcgt tattcgtccg ctggcctact gccgcgagaa agatattcag cgatttgccg
     1141 atgcaaaagc gttcccgatt attccgtgca acctgtgcgg ttcacagcct aacctgcaac
     1201 gtcaggtgat tgctgacatg ttgcgtgact gggataaacg ttatccaggg cgtatcgaga
     1261 cgatgttcag cgcgatgcag aatgtggtgc cgtcgcatct gtgcgatacc aacctgttcg
     1321 atttcaaagg catcacccac ggttctgaag tggttaacgg gggtgatctg gcgtttgatc
     1381 gcgaagagat cccactacaa ccggcgggct ggcagccaga agaagatgaa aatcagttgg
     1441 atgagttacg gctgaatgtg gttgaagtga aataaccagg atggcgcccg atgcgcaagc
     1501 gtatcgggct actcttatgg aggccggata agacgcggcc agcgtcgcat ccggcaatcc
     1561 cgaataagat gtttactctt gcacccggca attcaacatt tcattatttt aataaccgca
     1621 cccggctcgt ttttccttta atcttcccgc cctgtaactg tttccatgct ttatgagcaa
     1681 cagcctgacg gaccgcgaca tagacatgcg ccggatgcac ggcgattttg ccaatatctg
     1741 cgccatcaag cccgatatct cctgtcagtg cacctaatac atcacccggg cgcattttgg
     1801 cttttttccc accatcgata cacaacgttg ccatttctgc ttccagcggc acaatggaac
     1861 tattagctgg cggcgtttgc cagttaagtt ttatctgcaa catgtcagaa atgatattgg
     1921 cccgctgtgc ttcttccgga gcacagaaac tgatcgccag accgctattt cctgcacgag
     1981 ctgtacggcc gatgcgatgt acatgaactt cagggtccca cgccagctca aagttcacca
     2041 ccagctcaag cgatttaata tccagaccac gcgcagcaac atcagtcgca accagtacac
     2101 gggcgctacc gttagcaaaa cgtaccaggg tctgatcgcg atcgcgttgc tccaaatcgc
     2161 cgtgtaatga caatgcactt tgccctactt cattcagcgc gtcgcagaca gcctggcaat
     2221 cttttttggt attgcaaaac accacgcaag aggatggctg atgcaagctt aataaccgtt
     2281 gcaacagagg aattttgcct ttgctggatg tctcataaaa ttgttgttca atgggtggca
     2341 aagcatctgt tgagtcaatt tcaatcgcca aaggatcgcg ttgcactcgt ccgctgatta
     2401 cggcaatggc ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa
     2461 aacggatgac atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat
     2521 ccatcaccaa agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca
     2581 aacgacccgg cgttgccacg ataatatgcg gcgcatgttg caacgaatca cgctgcatac
     2641 cgaacggttg accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc
     2701 gacgcaattc acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct
     2761 gggtttgaaa tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt
     2821 tgccgctgcc ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg
     2881 cggcggcctg caccggcgtc atagttaaat aacccaactc attaaggttc gtgagttggg
     2941 cgggaggcaa aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac
     3001 gcaaagttag caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg
     3061 ttcttcatcg ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg
     3121 aacaggatcg ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat
     3181 atttccctcc ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa
     3241 aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa
     3301 aaaaggaagt aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca
     3361 agggagatat tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt
     3421 ttttgtatac agacagccgg aaaaattgct tttgttacaa ccatttacta cggtgcaacc
     3481 ataaagcaac accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt
     3541 gccacccgcc gccagggatc ccaccaaggt tgacgccaaa taacccggtc agaaaggtac
     3601 tgggtaaaaa gaccattgcc atcaacgaca ttgtataggt acgacgagct aaattttcct
     3661 gcatcacctg agcgatttca tccgccatca cgccagtccg tgctatacag gcgtcgattt
     3721 cgtcaaggcc gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc
     3781 tcatccacgg caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat
     3841 agcgacgcat cacaattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt
     3901 gctgatcaag gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt
     3961 cgctggaatg atcggtcaac gcatcgcaca catccaccag ccaccctccg caatcggtcg
     4021 gacccgtgcc ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc
     4081 gggtcgaaac aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc
     4141 gttcatcggt gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgagacggc
     4201 tgactcgggg ccgcgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg
     4261 gtgttgtcgc cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg
     4321 gatgcgcttc atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca
     4381 gcatccaggc aaatactgca tccgggacat taacgtccga tcccttaatc gcttccacag
     4441 tgcctccatc atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc
     4501 tcatcacccc attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc
     4561 aaaatgcttt tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa
     4621 ttatctgatc atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat
     4681 aacgggatct caatgattac gcacaacttc aataccctgg acttactcac cagtcctgtc
     4741 tggatcgttt cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg
     4801 atgcaagacc tcacgtttag tcagctacga accggtccct attccgtctc ctcacaaaaa
     4861 gaactgccga aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact
     4921 gttcagcgta aagaagagga aacagcatta aactgtcggc ttgttttgcg aaagctgaca
     4981 gaagcagaac cggtgattat tttcgaaggt atcgaagcgt cggcaacgct gggtttaaaa
     5041 gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg
     5101 actaactctg caccaatgtt gttgattgac ccctcacgag atggccaaat cgtcgatgct
     5161 aacctcgccg cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc
     5221 tgggaaataa atatgctcgg gcgtcgcgtc atgcctatca tgcatgaaat ttcgcattta
     5281 cccggtggtc ataaacctct gaattttatt cataagctgg cggatggttc gactcgtcat
     5341 gtgcagacct acgccgggcc gattgaaatt tatggcgaca agctcatgtt atgtattgta
     5401 catgatatta ctgagcaaaa acggctggag gagcagctgg aacatgctgc ccaccatgac
     5461 gcgatgaccg gattactgaa tcggcgacag ttttatcaca ttaccgagcc aggccaaatg
     5521 cagcatctcg ccatcgccca ggattacagc ttgttgctca tcgacaccga tcgttttaaa
     5581 cacattaacg atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc
     5641 accctcgaaa gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt
     5701 gtcttattgc taccaagaac cccactggat accgcgcttt cgctggctga aactatccgc
     5761 gtaagcgtgg caaaagtgag tatttcgggc ttaccacgct ttaccgtcag cattggtgtg
     5821 gcgcatcacg aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg
     5881 tatcgggcga aaaatgatgg acgcaaccgc gtgctggcgg cataagccgc ggatgcgtct
     5941 cgagatcaac gactgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgccgtt
     6001 ttacgtagtg cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc agtgcaatat
     6061 gcctgaacat catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca
     6121 tgcgatttat catcccgtcc tttgccatga ataatcagca cgttacgcaa accatcagcc
     6181 atggcttgtt gaatgaaact gaacatcatt ttgcggcact cttccaccgg ctggcgcaaa
     6241 agattcaggc tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgt
     6301 tgcaaccctt cccgccgaaa ctccagcggc tgacttagtg ggatgatgtc gagaaatccg
     6361 gtggtgagga aattatcaag ctgcagcgtg tcgatacgct gcggcgcacg ttggttacgc
     6421 gttggatgcc agtggacatc ggtagcacgt ttcagcggct ggacatcttc catggcgtca
     6481 agaaacagcg atttgtcgtc aaggttcatg ttacatcctc ccgcaattaa gagcgcgata
     6541 tgataaccag accgggtcgg tccaacaacg tattacccaa aattccagta ataagttcca
     6601 aatattgccg atattttaag caaaatgctt atgcatgatt attcattcac gatattaata
     6661 atgtaactta tattttcgtg aaatctgtca ctgaagaaaa ttggcaacta aaggttaaaa
     6721 ccgttataac acagtcaccg gcgcagagga gacaatgccg gatttaagac gcggatgcac
     6781 tgctgtgtgt actgtagagt ctggcggatg tcgacagact ctattttttt atgcagtttt
     6841 aactttgcag atagccgcat tctcgccgga attgcgtgat taatgacgcg gtcagtggtg
     6901 tctggcgact atcacgccgc tgaatcaaat aataggccgc tttcggtaaa atttcgctaa
     6961 ccggcaacat caccagcccc tgtccgtgca agggatcgca gcccatttct tcaggcagta
     7021 tgctgagaaa atcgcttttt gccaccagac tgatacaggc tgagaacgtc tcgcagacta
     7081 caccgacctg tggcgtttgc gcctgatcgt caagcaattc actcaactgt ttgtagtaac
     7141 tgccgtgtgg cgtcggcatt gtccagctgt aatccagtaa ctgtttgatc gaacgggcac
     7201 caatggcggg gtgtcccggg cggcaaaaga tcgcgaattg cttttccagt aatttctcaa
     7261 aagtaaattc gtggtcgtac ggtccctgat aataggtatt gatggtgaaa tccaattctc
     7321 cctgacgcaa ttcattaatc atcgacacca gttgcccttc cataatgcgt acttttacct
     7381 gcggatgctg ctgatgaaaa cgagatatga cagctggcat cagactgcgg gaaatactgg
     7441 cccccatgcc gatattaatc tgccctgcca gttgcccttg tcgttggcga atatcctctt
     7501 gggctgcgcg cagctcttca agaataagac tggcgtgctg ataaaaactt tcaccggcat
     7561 cagttaacgt cacgccttta ctacggcgaa aaaagagttg cgccgctaat ccttcttcca
     7621 gctcctgaat agatttactc agtgccggtt gcgacatatt caacattcgg ctcgctccgc
     7681 gaatgctgcc ctgacgagcc acttcaacaa aagcccgaat ttgatgaatt tttacctgaa
     7741 aagccatgac gccaccgata accgttattt atcagaccaa agaaactggc atctacttta
     7801 atgcagatga ttgtgtcagg gtaatttatg aacggttaaa actgtgaaaa atcagttagt
     7861 gataagtaaa aactatcgct acgtgaaccg ggtcacactt tttactgatg acgggaaagg
     7921 ttatggagtc tttgaatcaa tttgttaatt cgcttgcccc aaaattatcg cactggcgac
     7981 gtgattttca tcactatgca gagtctggct gggtggaatt ccgcactgcc acccttgttg
     8041 cggaagaatt gcagcagctc ggctattcac tggcgctggg ccgcgaagta gttaatgaaa
     8101 gtagccggat gggactacct gatgaactca ctctacaacg cgaattcgag cgcgctcgtc
     8161 aacagggtgc gctagcacaa tggattgcgg tttttgaagg tggtttcact ggtatcgtcg
     8221 ccaccctgga taccggtcgc cccggtccgg tgatggcttt ccgtgtcgat atggacgcgc
     8281 tggatctcag tgaagagcag gatgtcagcc atcgccccta ccgcgacggt tttgcgtcat
     8341 gtaacgccgg aatgatgcat gcctgtggtc atgatggaca taccgccatt gggcttgggc
     8401 tggcgcatac ccttaaacaa ttcgagtccg gactacatgg cgtcatcaaa ctgatttttc
     8461 agcctgcaga ggaaggtacg cgtggcgcgc gggcgatggt cgatgcaggt gtcgtagatg
     8521 atgttgatta ttttactgcc gtgcacattg gcactggcgt acctgcgggc accgtggtgt
     8581 gcggcagtga taattttatg gcaaccacca aatttgacgc gcacttcacc ggtaccgccg
     8641 ctcacgcagg cgcaaaacca gaagacggtc acaatgcctt gttggcggca gcacaagcca
     8701 ctcttgcact gcatgcaatc gccccgcaca gcgaaggagc ttccagagta aacgtgggcg
     8761 ttatgcaggc aggaagcggt cgtaacgttg ttcctgcctc ggcgttgctg aaagtggaaa
     8821 cacgcggggc cagcgacgtc attaatcaat atgtttttga ccgtgcacaa caagcgattc
     8881 agggcgcagc aaccatgtat ggtgtcggcg ttgaaactcg tctgatgggt gcagttaccg
     8941 ccagttctcc ttcgccgcaa tgggtcgcat ggttgcaaag tcaggcggct caggtcgcgg
     9001 gggtcaatca ggccattgaa cgtgttgaag cgcctgcggg ttccgaagat gccacattaa
     9061 tgatggcccg cgtgcagcaa catcaagggc aagcctccta cgtggtgttt ggcacacagc
     9121 tggcggcagg tcatcacaac gaaaaattcg attttgacga gcaggttctc gctattgccg
     9181 tcgaaacgct ggcgcgcacc gcgctcaatt ttccctggac gcgaggtatc tgatgcagga
     9241 aatctatcgt tttatcgacg atgcgattga agccgatcgc caacgttata ccgatattgc
     9301 cgatcaaatc tgggatcatc cagaaacacg ttttgaagag ttctggtcag cggagcatct
     9361 ggcttcggct ctggaatctg caggcttcac cgttacccgc aacgtaggca atatcccaaa
     9421 tgcctttatt gcttcgtttg gtcaaggcaa accggttatc gccctgctgg gagaatatga
     9481 cgccctggca ggtttaagtc agcaagctgg ttgcgcgcaa cctacatccg cgacgcccgg
     9541 tgaaaatggt cacggttgcg gacacaattt gcggggaacc gccgcctttg ccgctgcaat
     9601 agccgtcaag aaatggctgg aacaatatgg gcaaggcggc acagtgcgct tttatggttg
     9661 tcctggcgaa gaaggcggct cgggtaaaac gttcatggtc cgcgaggggg tatttgatga
     9721 tgtggatgcg gcactcacct ggcacccgga agcctttgcc ggtatgttca atacccgcac
     9781 gctggcaaac attcaggcat catggcgctt taaagggatc gcagcacatg ccgcgaattc
     9841 ccctcatttg ggacgcagcg cccttgatgc cgtaacgttg atgaccactg gcaccaactt
     9901 cctcaacgaa catattattg aaaaagcgcg cgtacactat gccatcacaa atagcggcgg
     9961 gatctcgccc aacgtggtcc aggcgcaggc agaagtgctt tatcttatcc gcgcccccga
    10021 aatgaccgac gtgcagcata tttatgatcg ggtcgccaaa atcgccgaag gtgcggcatt
    10081 gatgaccgaa accacggttg aatgccgctt cgacaaagcc tgttccagtt atctcccgaa
    10141 tcgcacctta gaaaatgcca tgtaccatgc cctatcccat tttggtaccc cggaatggaa
    10201 ctccgaagaa ctggcttttg cgaaacaaat tcaggctacg ctcaccccca acgatcggca
    10261 aaacagtctg aataatatcg ccgcaaccgg tggcgaaaac ggcaaggctt ttgcactacg
    10321 tcatcgtgaa acggtactgg cgaatgaggt cgctccatat gccgccaccg ataacgtgct
    10381 tgcggcatcg actgatgtcg gcgacgttag ttggaaactg cctgttgccc agtgtttcag
    10441 cccctgtttt gccgtcggta caccgctaca tacgtggcaa ctggttagcc aggggcgaac
    10501 atctattgct cataaaggaa tgctgctggc ggcgaaaact atggcagcaa ccacagtcaa
    10561 tctcttcatt gattcagggc tattgcaaga atgccaacaa gagcatcagc aagtaacgga
    10621 cacgcaaccg tatcactgcc ctatcccgaa aaacgtgaca ccgtcacctt taaaataaca
    10681 acaacaacgc aaacacaaca accgaggaat gcccatgagt atgtcatcca taccgtcgtc
    10741 ctcccaatcc gggaagctct atggctgggt cgaaagaatt ggtaacaagg ttctccatcc
    10801 ttttttgctc tttatctatt tgattatcgt actcatggtg acgacggcaa ttttgtcggc
    10861 ctttggcgtc agtgcgaaaa acccgaccga tggaacgccg gtggtggtga aaaacctgct
    10921 cagtgtggaa ggattacact ggtctttacc caatgttatt aaaaacttta gcggttttgc
    10981 tccacttggt gcgatcctgg cgctggtttt aggtgccggt ctggcggagc gcgtcggctt
    11041 actgccagcg ctaatggtta aaatggcatc gcatgttaat gcccgctacg ccagttatat
    11101 ggtgctgttt attgcttttt tcagccacat ttcttccgat gcgacgttag tgatcatgct
    11161 accgatgggt gcgctgattt ttctggcggt ggacaggcat ccagttgcag gtttactggc
    11221 tgccattgca ggcgtaggtt gcggctttac ggctaattta ctgattgtca caaccgacgt
    11281 gttgctgtcg gggatcagca cggaagcggc ggctgcgttc aatccgcaaa tgcacgtcag
    11341 tgtaattgat aactggtatt ttatggccag ctccgtagtc gtactgacga ttgttggcgg
    11401 cctgataacc gacaaaatca tcgagccacg gttaggtcaa tggcagggaa acagcgatga
    11461 gaaactgcag acattgaccg aaagtcagcg ttttggttta cgcatagcag gtgtcgtatc
    11521 gctacttttt attgctgcga ttgcgctgat ggtgatcccg gaaaacggga tattgcgcga
    11581 tccgattaat cacaccgtga tgccatcacc ctttattaaa ggtatcgtgc cactgatcat
    11641 tctttttttc tt
//
LOCUS       DAJVQG010000123        11647 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJVQG010000123 DAJVQG010000000
VERSION     DAJVQG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11647
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.123"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            131..1045
                     /locus_tag="OQM83_003046"
     CDS             131..1045
                     /locus_tag="OQM83_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1521249.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAADGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1379..3658
                     /gene="ydeP"
                     /locus_tag="OQM83_003047"
     CDS             1379..3658
                     /gene="ydeP"
                     /locus_tag="OQM83_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCS1521250.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3906..4103
                     /gene="safA"
                     /locus_tag="OQM83_003048"
     CDS             3906..4103
                     /gene="safA"
                     /locus_tag="OQM83_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCS1521251.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            4178..4939
                     /gene="ydeO"
                     /locus_tag="OQM83_003049"
     CDS             4178..4939
                     /gene="ydeO"
                     /locus_tag="OQM83_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCS1521252.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
     gene            5340..7022
                     /locus_tag="OQM83_003050"
     CDS             5340..7022
                     /locus_tag="OQM83_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCS1521253.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            7074..8231
                     /locus_tag="OQM83_003051"
     CDS             7074..8231
                     /locus_tag="OQM83_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCS1521254.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPLELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(8197..8319)
                     /locus_tag="OQM83_003052"
     CDS             complement(8197..8319)
                     /locus_tag="OQM83_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521255.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            8528..9487
                     /locus_tag="OQM83_003053"
                     /pseudo
     CDS             8528..9487
                     /locus_tag="OQM83_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(9508..10539)
                     /locus_tag="OQM83_003054"
     CDS             complement(9508..10539)
                     /locus_tag="OQM83_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HCS1521256.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
     gene            complement(10642..11647)
                     /locus_tag="OQM83_003055"
                     /pseudo
     CDS             complement(10642..11647)
                     /locus_tag="OQM83_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
BASE COUNT         3245 a         2586 c         2530 g         3286 t
ORIGIN      
        1 atcacggtga atggaaacgc aagctaggga acgatcgagg cgctaatcaa tgtgatctac
       61 acctggcaat aacgtcgatt cgattttctt agccttcttt ttaaatcaat ttttaacctt
      121 aggatatatc atgggtaaaa caatcagtat aaaggtcctg ttcggtattt atcttctatt
      181 aatggcaggc aaagtttttg ccttcagttg taatgtcgat ggcgggtcaa gtattggagc
      241 aggtacaacc tctgtttatg ttaatctcga ccctgtaata cagccgggcc agaatctggt
      301 tgtagacttg tctcagcata taagttgctg gaatgattac ggcggctggt acgacactga
      361 tcatataaac ctggtacaag gctcagcttt cgcaggctcc ctacagtcat ataaaggatc
      421 gctgtactgg aataatgtga cctacccctt cccgttgacg acaaatacca acgtattgga
      481 tattggtgat aagacaccga tgccattacc gttaaaactc tatattaccc cagttggggc
      541 ggccgatgga gttgtcataa aagcggggga agtcattgcc cgtattcata tgtataaaat
      601 tgcaacatta ggaagcggaa atccgcgtaa ttttacgtgg aatattattt caaataacag
      661 tgttgttatg ccaacaggcg gctgcaccgt tgatagtcgt aatgtcactg tggatctccc
      721 ggactttcct ggaagcgcag aaatacccct tggcgtatat tgctcaagcg aacaaaagct
      781 ctcattttat ctttcgggtg ccaccactga cagtagccga caagtgtttg ctaacacagc
      841 acctgatgca acaaaagcat cgggagtggg tgtcactcta atgcgtaatg gaaaaatact
      901 ggctacggga gaaaatgtct ccctggggac agtaaataaa agcaaagtac ctctgggttt
      961 aagcgcaacc tatggacaaa caggaaacaa ggtttcagcc ggaaccgtcc agtcagtcat
     1021 tggtgtcact tttatctatg aataattgtg attttgacct tagtctatgc gctccattca
     1081 cgaaaattgc gcagataatt attttgtgaa ggctattatc ctacacctgt aagaaaatcc
     1141 gcgcacaatt gaagccagag tcttattttt tatattgaaa aatatgttaa tttaaacact
     1201 atgagagatg atgcttaact tatcgtattt aatctattgt ttaacgataa tagattaaaa
     1261 atagaaagag agtagtcccc tactcttata tatccatgtt ggcgataatc cctttgtatg
     1321 tactgtgcat catcgctatt acaaatccta ataattcatt tccacacagg ataagtagat
     1381 gaagaaaaaa attgaatcct accagggtgc tgcaggtggt tggggtgctg ttaaatccgt
     1441 agcgaatgca gtacgtaagc agatggatat acgccatgat gttattgcca tgtttgacat
     1501 gaataagcca gagggctttg actgtccggg ttgtgcatgg ccagatccta agcacagtgc
     1561 gtcattcgac atttgtgaaa acggcgcaaa agcaatcgcc tgggaagtca cggataagca
     1621 ggtaaatgcc tctttctttg ctgagaatac ggttcaatca ttacttacct ggggagatca
     1681 cgagcttgag gctgcggggc gtctcactca gcctttgaaa tatgatgccg tcagcgactg
     1741 ttacaagcca ttaagctggc aacaagcttt cgacgaaatt ggcgcacgcc ttcaaagcta
     1801 tagtgatccc aatcaggttg aattctatac ttcgggccgc acttccaatg aagctgcctt
     1861 tctttatcag ctttttgccc gtgaatacgg gagcaataac tttcccgact gctccaacat
     1921 gtgccatgaa ccgacaagcg tgggtttggc agcgagtatc ggtgtaggta aagggaccgt
     1981 gttgctggaa gactttgaga agtgcgattt agtcatttgc attgggcata accctggtac
     2041 aaaccaccct cgcatgctga cttcgttgcg cgctttagtg aaacggggag cgaaaatgat
     2101 cgccatcaat cctctacagg aacgtggcct ggagcgattt accgcaccgc aaaacccgtt
     2161 tgaaatgctg acgaactctg agactcagtt ggccagtgcc tactacaacg tgcgcattgg
     2221 tggcgatatg gcgttgctca aggggatgat gcgcctgtta attgagcgcg atgatgctgc
     2281 aagtgccgca ggtcggccct cattgcttga tgacgaattt attcaaacgc ataccgtcgg
     2341 ctttgacgag ctacgccgtg acgttctcaa ttccgagtgg aaagatatcg aacgtatttc
     2401 tggactaagt cagacacaaa tcgccgaact ggctgacgca tatgccgctg ccgatcgcac
     2461 cattatctgt tacggaatgg ggatcactca gcacgaacat ggtacccaga atgtacagca
     2521 actggtcaat ctgctgttga tgaaaggtaa cattggcaag cctggtgcgg gtatctgccc
     2581 actacgaggt cactctaatg tacagggcga ccgaaccgtc ggtatcaccg agaaaccgtc
     2641 tgcagagttt ctggctcgcc tgggtgagcg ctatggcttc accccacctc atgcacctgg
     2701 acatgctgca attgccagca tgcaagcaat atgcacgggg caggctcgag cattgatctg
     2761 catggggggc aactttgcac tggcaatgcc agatcgggaa gcgagcgctg taccgttaac
     2821 gcaattagat ttggcggtac acgtagccac taagcttaac cgctctcatc tgctgaccgc
     2881 acggcatagc tatattctgc cggttctggg gcgtagcgag attgacatgc aaaaaagcgg
     2941 tgcgcaggcg gtaaccgttg aggattcaat gtcgatgatt catgcctcgc gtggcgtgtt
     3001 aaaacccgcc ggtgtaatgc tgaaatcaga gtgtgcagtg gtcgcgggaa tcgcgcaggc
     3061 agcactaccc cacagcgtgg tagcctggga gtatctggtg gaagattatg atcgcattcg
     3121 caatgacatt gaagctgtgt taccagagtt cgccgactat aaccagcgca tccgacatcc
     3181 tggaggtttt cacctgataa atgcagctgc tgaaaggcgc tggatgacgc cgtcaggtaa
     3241 ggctaatttc attaccagca aagggctgtt agaagatccc tcttcagcgt ttaacagtaa
     3301 gctggtcatg gcgacagtac gtagccacga tcagtacaac acgacgattt atggtatgga
     3361 tgatcgctat cgaggggtat tcggtcaacg agatgtggtc tttatgagtg ctaaacaagc
     3421 taaaatttgc cgtgtaaaaa acggcgaaag agttaatctt attgcgctta cgccagacgg
     3481 taagcgcagc tcacgccgca tggatagatt aaaagtggtc atttacccta tggctgaccg
     3541 ctcactggtg acctattttc cagaatcgaa tcacatgcta actcttgata accacgatcc
     3601 attaagtggc attcctggtt ataaaagtat tccggttgaa ttagaaccat caaattaatt
     3661 cgcctcctca tttcttctgc tgtcatccgc acagcagaag aatttctcat tgactattat
     3721 cgcgcaattt gctcacattg attaaattaa accacacact ataagatata aacctctgcc
     3781 tacagctgta agaaactccg ctcagtactg aagtgccagt cctatttcct cttttctcca
     3841 gcctgttata ttaagcatac tgattaacga tttttaacgt tatccgctaa ataaacatat
     3901 ttgaaatgta tgcgaccaca gtgaaaaaca aaatcacgca aagagacaac tataaagaaa
     3961 tcatgtctgt aattgtggtt gtcttattac tgacccttac gttgatagcc attttttcgg
     4021 caattgatca gctgggtatt tcagaaatgg gtcgcattgc aagagatctt acacatttca
     4081 ttatcaatag tttgcaagac tgaaaataaa cagcaaatta taaatatgaa atgttaaaaa
     4141 agtatcgata aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgttat
     4201 atttattcat catgccttca acgctaacat tttagataaa gattacgcct tctctgacgg
     4261 cgagatcctg atggtagata acgctgttcg tacgcatttt gaaccttatg agcggcattt
     4321 taaagagatc ggatttactg aaaataccat taaaaaatat ctacaatgca ctaacatcca
     4381 gacagtgacg gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt
     4441 gcttaatgaa atgattgctt atcttaactc ggaagaacgc aatcatcata atttttcaga
     4501 acttttgctt ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt
     4561 aactaacggt gtgctatccg tttccggaaa agtgagaaat attgtcaaca tgaagctggc
     4621 gcacccatgg aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa
     4681 gaaaaaacta aagcaagagc aaacgacatt ctcacagatt ctattagatg ccagaatgca
     4741 gcacgcaaaa aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg
     4801 ttatgccagt acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa
     4861 gagagtttct aaggagtacc gttgtcaaag tcacatgggt atgaatacgg gcaacacgat
     4921 gagtgcttta gctatttgat tatttgctaa cgagtagtca accacgcacg ctgcgtaaga
     4981 attaaatggg gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat
     5041 aaataagtga catccatcac atatttatgc acttgcataa cctgttgcag attatttatg
     5101 atctcaattc ggcattttgt cagtaaaatg caataattta ttaaatatca ataaattagt
     5161 tgtttatcgg cgagaaatta cttaatagaa cagaaagtaa tgtcaacgct ttatggactg
     5221 ttatttccct ttttttagct aaatctgcta tctctttatg tgactaactt cacttacatc
     5281 cacttatttc tcttcgtaaa attacttcgg aattaagtac aataagaaga ggaacattta
     5341 tgaagtctgc attaaagaaa agtgtcgtaa gtacctcgat atctttgata ctggcatctg
     5401 gtatggctgc atttgctgct catgcggcag atgatgtaaa gctgaaagca accaaaacaa
     5461 acgttgcttt ctcagatttt acgccgactg aatacagtac caaaggaaag ccaaatatta
     5521 tcgtactaac catggatgat cttggttatg gacaacttcc ttttgataag ggatcttttg
     5581 acccaaaaac aatggaaaat cgtgaagttg tcgataccta caaaataggg atagataaag
     5641 ccattgaagc tgcacaaaaa tcaacgccga cgctcctttc attaatggat gaaggcgtac
     5701 gttttactaa cggctatgtg gcacacggtg tttccggccc ctcccgtgcc gcaataatga
     5761 ccggtcgagc tcccgcccgc tttggtgtct attccaatac cgatgctcag gatggtattc
     5821 cgctaacaga aactttcttg cctgaattat tccagaatca tggttattac actgcagcag
     5881 taggtaaatg gcacttgtca aaaatcagta atgtgccggt accggaagat aaacaaacgc
     5941 gtgactatca tgacaacttc accacatttt ctgcggaaga atggcaacct caaaaccgtg
     6001 gctttgatta ctttatggga ttccacgctg caggaacggc atattacaac tccccttcac
     6061 tgttcaaaaa tcgtgaacgt gtccccgcaa aaggttatat cagcgatcag ttaaccgatg
     6121 aggcaattgg cgttgttgat cgtgccaaaa cacttgacca gccttttatg ctttacctgg
     6181 cttataatgc tccgcaccta ccaaatgata atcctgcacc ggatcaatat cagaagcaat
     6241 ttaataccgg tagtcaaaca gcagataact actacgcttc cgtttattct gttgatcagg
     6301 gtgtaaaacg cattctcgaa caactgaaga aaaacggaca gtatgacaat acaattattc
     6361 tctttacctc cgataatggt gcggttatcg atggtcctct gccgctgaac ggggcgcaaa
     6421 aaggctataa gagtcagacc tatcctggcg gtactcacac cccaatgttt atgtggtgga
     6481 aaggaaaact tcaacccggt aattatgaca agctgatttc cgcaatggat ttctacccaa
     6541 cagctcttga tgcagccgat atcagcattc caaaagacct taagctggat ggtgtttcct
     6601 tgctgccctg gttgcaagat aagaaacaag gcgcgccaca taaaaatctg acctggataa
     6661 cctcttattc tcactggttt gatgaggaaa atattccatt ctgggataat taccacaaat
     6721 ttgttcgcca tcagtcagac gattacccgc ataaccccaa cactgaggac ttaagccaat
     6781 tctcttatac ggtgagaaat aacgattatt cgcttgtcta tacagtagaa aacaatcagt
     6841 taggtctcta caaactgacg gatctacagc aaaaagataa ccttgccgcc gccaatccgc
     6901 aggtcgttaa agagatgcaa ggcgtggtaa gagagtttat cgacagcagc cagccaccgc
     6961 ttagcgaggt aaatcaggag aagtttaaca atatcaagaa agcactaagc gaagcgaaat
     7021 aactaaacct tcatgcggcg gatttttccg ccgccttatt gagcgagata gcgatgcacg
     7081 ttacagccaa gccctccagt tttcaatgta atctcaaatg tgattactgt ttttaccttg
     7141 aaaaagagtc gcagtttact catgaaaaat ggatggatga cagcactctg aaagagttca
     7201 tcaaacaata tatcgaagcg tctggcaatc aggtctattt tacctggcaa ggcggtgaac
     7261 ccactctggc tggcctggat tttttccgta aagttattca ctatcaacaa cgctatgcag
     7321 gccaaaaacg tatttttaat gcattacaaa cgaatggcat tttattgaat aatgaatggt
     7381 gtgcctttct caaagaacat gaatttctgg ttggtatctc gatcgatggc cccctggagt
     7441 tacatgaccg ttacagacgc agtaattcag gtaacggtac ttttgcaaaa gtgatagcag
     7501 ccatcgagcg tctgaaatca tatcaagtag agtttaatac gttaaccgtc attaataacg
     7561 ttaatgtcca ttaccctctt gaggtttatc attttttaaa atctatcggc agtaaacata
     7621 tgcaatttat cgaattgcta gaaaccggga cgccgaatat tgatttcagt ggtcatagtg
     7681 agaacacatt ccgtatcatt gatttttctg tgcctcccac ggcttatggc aagtttatgt
     7741 caaccatttt tatgcaatgg gttaaaaacg atgtgggtga aattttcatt cgtcagtttg
     7801 aaagctttgt cagccgtttt ttggggaatg ggcatacaag ttgtattttc caggagtcct
     7861 gcaaggataa tctggttgtt gaaagtaatg gagacattta cgaatgcgac cattttgtct
     7921 atccacagta caaaattgga aacattaata aatctgaact caaaacgatg aacagtgtac
     7981 aactgacagc gcaaaaaaaa cggatttcag cgaaatgtca gcaatgtgca tataaaccta
     8041 tctgcaatgg cggttgtcct aagcatcgta ttactaaagt aaacaatgag actgtttctt
     8101 atttttgcga aggttataaa atcctttttt caaccatggt accttatatg aacgccatgg
     8161 ttgagttagc taagaacaga gtaccgcttt accacattat ggatgttgca aaacaaatgg
     8221 agaataatta ataacgctct cttttaactc cattcttagc ctccttctta tgaccttaac
     8281 aaggcacccg acataaattc ctgtcgggtg tttcgtcatc atcatcttcc cttgcgaaaa
     8341 tcctggctta cgtggtatgc gtcagggcat tagcaatacc cttgattatc aatagcataa
     8401 ccataaccat aaccactgaa aattaaaaat ttctatatat ttcatattgt taaatgatac
     8461 ttaaataaat ttcatcagta aatcattgcc atagataatc attggatggc acaatcaatt
     8521 ttcgctaatg ataaccattc ccattacgtt ttatatgttg atagcaaagt atctctgtct
     8581 gctgaagccc ttttggctgc gtaagaataa taaaacttca gtgttgttaa tcattattat
     8641 cctcgcaatg atccttggcg tggttaaaat tcaggtctgg ttaaacgact ggaataatga
     8701 ttttttcaat gcgctaagcc agaaagaaac cgataagctt tggcagcttg ttctttggtt
     8761 ccctgccctg ctggggatct ttgtattaat atccgtaaac aaaacatggt taattaaact
     8821 actgactatt cgctggcgcg aatggttaac agattactac cttaatcgct ggtttgcaga
     8881 taaaaactac tacttcacgc agatctacgg cgagcataaa aatacagata accctgacca
     8941 acgtatcgct gaagacattc tcttactgat cagcaaaacg ctcagtcttt catttggctt
     9001 catccagtca cttagcatgc tgatcacctt taccgttatt ctttggcaaa gcgcgggtac
     9061 gctctcattc actgttggtg gaacagaatg gaatatccag gggtatatgg tctataccgt
     9121 cgtgctcatt gtgatcggtg gaactttatt tacccataag gtgggtaaac gtattcgtcc
     9181 gcttaatgtg gaaaaacaac gtagcgaagc gacatttcga actaatcttg tgcagcataa
     9241 taagcagaca gaactgattg cgctaagcaa tgctgagtct ctacaacgcc aggagttgcg
     9301 cgacaatttt cataccatta aagagaactg gcatcgttta atgaatcgtc agcggtggct
     9361 tgattactgg caaaatattt attcgcgctc gcttagcgtt cttccctact ttctgttatt
     9421 gccgcagttt attagcgggc agatcaatct gggcggactg atgaaatcgc gccaggcatt
     9481 tatgctaaat agctgcgctt aataccgcta cacttttgcc agcccatgtt tgcctccggg
     9541 gaatgggctg acggtttcca taaaatggcg aacttttttc aacagttgcc acattgagct
     9601 gcactgatga ttacgcgtta ttgtgtcgtg aagtgcctgc catagccgtt caacatgatt
     9661 cacccatggc gagtaaaccg gctgataaat gaccctgaac ttcgggttct ccttcagcca
     9721 gctctgtgtt tcccggcttt tgtggataat gtagttgtcc acgatcagcg tgatggtttt
     9781 cgcccgacgg tatgtcgctt taagccgctt cagcaggctg atgaacagcg ccgaactttt
     9841 gctgttgccg cccacacagc tgactttacc tgtcccgctg tgcagcgctc cggccagata
     9901 atatttttca ttctgtcccg gcgtgaccac ccgtttttgc tgtccgcgca gttgccagtc
     9961 cgcaccgatt ttgggattaa gatggatatc cacttcatct tcataaaaga ccggatgctc
    10021 tgcgctgcat tcgtccagtg ctttatggat tgctgccatc ttttcatctt tatgcgggtc
    10081 acggatacgc agagttggcg cagcccttcg ccacacaatc cccgcagacg gcaaccagcg
    10141 gcgaacggtt ccggcattta actggcaacc ggttatctca ttgattttta ttgccagcag
    10201 ttctgtactc cagcgtgaac gctggtagcc aaagtcgccg ggagaatgtt ttaccagctc
    10261 acgtaacagt gtgcagatat gctcaaacgg ccagcgacgg gcacgcccgg caggtaatga
    10321 tttcagtccc tcaacacccg actgcgtgaa ccagttaatc cagcgtccaa cagaggaacg
    10381 ggcgcagcag agcgttctgg caacgtcgct gacacggtcg ccccggtgca gcatcagcat
    10441 ggcagtcagt ctgcgggcat aatttttatc gtgtgtttta tggatggctt tctgcatcag
    10501 gcgtcgttcg tcacgggaaa ttggtgctat gatcggcatt gctcagtccg gttggtgatt
    10561 tgttttgatt tggcgattga tcagatcgca caatccgggc taagttccct caaagtgatc
    10621 tactattccg cgcagctatt tagatgacca atacatattt gattgaaaac cagcttcagt
    10681 cgtgtaatgc cccatatctc cccattcact gtacagtgac ccgatgcctc gggaactact
    10741 gtggtttaaa tagtttgctc tggggagcgt gtagccattg ctgctgcaaa aattttcagc
    10801 aaggctgtat atcatgaaag cctcgccggc gttcacccac caactcttca cacggatttc
    10861 gtatacatag ctagggcctc ctgattttgg tgtcgccgta atcctttccg aattgctgcc
    10921 gacattttta aatgtcactt taccggtagc atcaacatcg acccaggagg cagagcttga
    10981 aaactcataa tcagccgccg tttttcctgg ggcaaagttg tcattattca actgataata
    11041 cgccccggtg aacccctgcg aagggaatgt agttgtcggt aggtcagtac cattgactga
    11101 tactgtaccg ctcattattt tgtcttctgc gcgagtgaat tgtattgtgg tactcagacc
    11161 agcttgatga acaccattca atacagggat cagcgttgtg ataccctctc cgcctcctgt
    11221 aacagtggct ttgtactcgc ctgagaaatt tttactgtag tcaattgcac taacttgcac
    11281 atagggcacg ttggtacctg actgtacaaa ttccagccct tcagaaactt tgatcggatt
    11341 gcctgatata tcgtgcagaa caagatgtag ctcagcacta tcggtaaagt ctcctttcaa
    11401 tgatgaccga ttgttcttaa ggactgactt tgaggcgtct gctgggcctg ccaccagcgt
    11461 tatatctact gtttgcattc caccagcggt cgtgactgcg cttaccgtga cgctacctgt
    11521 catcgctcct ttcacgcttg ttgtcgcgat tccgttttga tcggtcaccg ctgttaatga
    11581 cgttaatgtg gcagagccgc tttttaaggc aaagtacaca gtgagacctt cgatcaggtt
    11641 accactg
//
LOCUS       DAJVQG010000124        11357 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJVQG010000124 DAJVQG010000000
VERSION     DAJVQG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11357
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.124"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..537)
                     /gene="espL1"
                     /locus_tag="OQM83_003056"
     CDS             complement(<1..537)
                     /gene="espL1"
                     /locus_tag="OQM83_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HCS1521257.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQYA"
     gene            1062..2990
                     /gene="thrS"
                     /locus_tag="OQM83_003057"
     CDS             1062..2990
                     /gene="thrS"
                     /locus_tag="OQM83_003057"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCS1521258.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2994..3536
                     /gene="infC"
                     /locus_tag="OQM83_003058"
     CDS             2994..3536
                     /gene="infC"
                     /locus_tag="OQM83_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCS1521259.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3633..3830
                     /gene="rpmI"
                     /locus_tag="OQM83_003059"
     CDS             3633..3830
                     /gene="rpmI"
                     /locus_tag="OQM83_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCS1521260.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3883..4239
                     /gene="rplT"
                     /locus_tag="OQM83_003060"
     CDS             3883..4239
                     /gene="rplT"
                     /locus_tag="OQM83_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCS1521261.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4356..4480
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4362..4406
                     /gene="pheM"
                     /locus_tag="OQM83_003061"
     CDS             4362..4406
                     /gene="pheM"
                     /locus_tag="OQM83_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCS1521262.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4689..5672
                     /gene="pheS"
                     /locus_tag="OQM83_003062"
     CDS             4689..5672
                     /gene="pheS"
                     /locus_tag="OQM83_003062"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCS1521263.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5687..8074
                     /gene="pheT"
                     /locus_tag="OQM83_003063"
     CDS             5687..8074
                     /gene="pheT"
                     /locus_tag="OQM83_003063"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCS1521264.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            8079..8378
                     /gene="ihfA"
                     /locus_tag="OQM83_003064"
     CDS             8079..8378
                     /gene="ihfA"
                     /locus_tag="OQM83_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCS1521265.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8479..9459
                     /gene="btuC"
                     /locus_tag="OQM83_003065"
     CDS             8479..9459
                     /gene="btuC"
                     /locus_tag="OQM83_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCS1521266.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9522..10073
                     /gene="btuE"
                     /locus_tag="OQM83_003066"
     CDS             9522..10073
                     /gene="btuE"
                     /locus_tag="OQM83_003066"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCS1521267.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPENPIVMESIKLALAK"
     gene            10073..10822
                     /gene="btuD"
                     /locus_tag="OQM83_003067"
     CDS             10073..10822
                     /gene="btuD"
                     /locus_tag="OQM83_003067"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCS1521268.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10900..>11357
                     /locus_tag="OQM83_003068"
     CDS             10900..>11357
                     /locus_tag="OQM83_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCS1521269.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
BASE COUNT         2873 a         2631 c         3057 g         2796 t
ORIGIN      
        1 ggcgtattgc aatgacagat ggcgacaaaa aatagcgtca gaaggagatt gcgaaaaaaa
       61 tgcactacca ttgagattta ctgttggttg tatagttttt tctctatgga tttttttaga
      121 cagtttcata caatggttac gatcttcggc agttaactgt tcctgttcaa tataatccag
      181 tatgcttcgc aaatggttag ccttaatagg cgtggtttcc agaaataagg gaatgatttc
      241 ttttttatag tgtaatgtaa tgagtatatt ttctgcatgg atgggtgaag tggcaaagtt
      301 aatgataaaa agggcagtgg aataacaatt ttgtgaaatt tttcgacagt ttgcggtaaa
      361 ttcctgagta acacagtatg acagcatcga acttaagtca gaggtatcaa gtagggtgat
      421 gagttggcgc gcaaattgat taataggctc tgatgaaggc gttggtatgt cgctatcgat
      481 gtgcaatcta tgaggagact tataatgggt tctgataatg atatcgtttt gactcataat
      541 ctattcgcct tatatatgtc tggaggtttt gaggatgatg attaattatt atcgccagaa
      601 cgaaatgtgc acgaaaaatt gctataagaa ttgtgtcact tttatcattg tgatatgacc
      661 acttcgtcat taatctcatg agcgtttaaa atactaccgc caaatcacgc aacgaacttt
      721 gctggattca gtttatgctg ctgtaaatcc gctcgcagta aacctttcag acgcaccgtg
      781 atgttatcag tggttcttta agcgttttgc tggtgtactc actacaaacg aattgcgaat
      841 cgaatcaatg tgaaacggaa aggtacaatc tccctttttt tagtgttaac atcgttcaac
      901 cgggtggaag gtgagaggtt aatgtctttc atttgtgtac ctgattgcga accaatttag
      961 catttgttgg ctagatggtt tcgcaatgaa ctgttaataa acaaattttt ctttgtatgt
     1021 gatctttcgt gtgggtcacc actgcaaatt aaggatataa aatgcctgtt ataactcttc
     1081 ctgatggcag ccaacgccat tacgaccacg ctgtaagccc catggatgtt gcgctggaca
     1141 ttggtccagg tctggcgaaa gcctgtatcg cagggcgcgt taatggcgaa ctggttgatg
     1201 cttgcgatct gattgaaaac gacgcacaac tgtcgatcat taccgccaaa gacgaagaag
     1261 gtctggagat cattcgtcac tcctgtgcgc acctgttagg gcatgcgatt aaacaacttt
     1321 ggccgcatac caaaatggca atcggcccgg ttattgacaa cggtttttat tacgacgttg
     1381 atcttgaccg cacgttaacc caggaagatg tcgaagcact cgagaagcgg atgcatgagc
     1441 ttgctgagaa aaactacgac gtcattaaga agaaagtcag ctggcacgaa gcgcgtgaaa
     1501 ctttcgccaa ccgtggggag agctacaaag tctccattct tgacgaaaac atcgcccatg
     1561 atgacaagcc aggtctgtac ttccatgaag aatatgtcga tatgtgccgc ggtccgcacg
     1621 taccgaacat gcgtttctgc catcatttca aactaatgaa aacggcaggg gcttactggc
     1681 gtggcgacag caacaacaaa atgttgcaac gtatttacgg tacggcgtgg gcagacaaaa
     1741 aagcacttaa cgcttacctg cagcgcctgg aagaagccgc gaaacgcgac caccgtaaaa
     1801 tcggtaaaca gctcgacctg taccatatgc aggaagaagc gccgggtatg gtattctggc
     1861 acaacgacgg ctggaccatc ttccgtgaac tggaagtgtt tgttcgttct aaactgaaag
     1921 agtaccagta tcaggaagtt aaaggtccgt tcatgatgga ccgtgtcctg tgggaaaaaa
     1981 ccggtcactg ggacaactac aaagatgcaa tgttcaccac atcttctgag aaccgtgaat
     2041 actgcattaa gccgatgaac tgcccgggtc acgtacaaat tttcaaccag gggctgaagt
     2101 cttatcgcga tctgccgctg cgtatggccg agtttggtag ctgccaccgt aacgagccgt
     2161 caggttcgct gcatggcctg atgcgcgtgc gtggatttac ccaggatgac gcgcatatct
     2221 tctgtactga agaacaaatt cgcgatgaag ttaacggatg tatccgttta gtctatgata
     2281 tgtacagcac ttttggcttc gagaagatcg tcgtcaaact ctccactcgt cctgaaaaac
     2341 gtattggcag cgacgaaatg tgggatcgtg ctgaggcgga cctggcggtt gcgctggaag
     2401 aaaacaacat cccgtttgaa tatcaactgg gtgaaggcgc tttctacggt ccgaaaattg
     2461 aatttaccct gtatgactgc ctcgatcgtg catggcagtg cggtacagta cagctggact
     2521 tctccttgcc gtctcgtctg agcgcctcct atgtgggcga agacaacgag cgtaaggtac
     2581 cggtaatgat tcaccgcgca attctggggt cgatggaacg tttcatcggt atcctgaccg
     2641 aagaattcgc tggtttcttc ccgacctggc ttgcgccagt tcaggttgtt atcatgaata
     2701 ttaccgattc acagtctgaa tacgttaacg aattgacgca aaaactatca aatgcgggca
     2761 ttcgtgttaa agcagacttg agaaatgaga agattggctt taaaatccgc gagcacactt
     2821 tgcgtcgcgt cccatatatg ttggtctgtg gtgataaaga ggtggaatca ggcaaagttg
     2881 ccgttcgcac ccgccgtggt aaagacctgg gaagcatgga cgtaaatgaa gtgatcgaga
     2941 agctgcaaca agagattcgc agccgcagtc ttaaacaatt ggaggaataa ggtattaaag
     3001 gcggaaaacg agttcaaacg gcgcgcccta accgtatcaa tggcgaaatt cgcgcccagg
     3061 aagttcgctt aacaggtctg gaaggcgagc agcttggtat tgtgagtctg agagaagctc
     3121 tggagaaagc agaagaagcc ggagtagact tagtcgagat cagccctaac gccgagccgc
     3181 cggtttgtcg tataatggat tacggcaaat tcctctatga aaagagcaag tcttctaagg
     3241 aacagaagaa aaagcaaaaa gttatccagg ttaaggaaat taaattccgt cctggtacag
     3301 atgaaggcga ctatcaggta aaactccgca gcctgattcg ctttctcgaa gagggtgata
     3361 aagccaaaat cacgctgcgt ttccgcggtc gtgagatggc gcaccagcaa atcggtatgg
     3421 aagtgcttaa tcgcgtgaaa gacgatttgc aagaactggc agtggtcgaa tccttcccaa
     3481 cgaagatcga aggccgccag atgatcatgg tgctcgctcc taagaagaaa cagtaaggcc
     3541 ttcaagtagc actgtctgtg gagccttcgg gcttcacagg ctttgttcgc ctttgtttcg
     3601 tttattaaca atgcgaagtg gaagttatta aaatgccaaa aattaagacc gtacgcggtg
     3661 ctgctaagcg cttcaaaaaa accggtaaag gtggttttaa gcacaagcac gctaacctgc
     3721 gtcacattct gaccaaaaaa gcgaccaaac gtaaacgtca cctgcgtccg aaagccatgg
     3781 tttccaaagg cgatctgggc ctggtaatcg cgtgcctgcc gtacgcataa gccgttaacg
     3841 tttttaactt tttaattaga atatagatac aggagagcac atatggctcg cgtaaaacgt
     3901 ggtgttattg cacgcgcacg tcacaagaaa attttgaaac aagctaaagg ctactacggt
     3961 gcgcgttctc gcgtataccg cgttgccttc caggctgtta tcaaagctgg tcagtatgct
     4021 taccgtgacc gtcgtcaacg taagcgtcag ttccgtcaac tgtggattgc gcgtatcaac
     4081 gcagcagcac gtcagaacgg tatttcttac agcaaattca tcaatggcct gaaaaaagcc
     4141 tctgttgaaa tcgaccgtaa gatcctggct gatatcgcag tattcgacaa agtagcgttc
     4201 accgctctgg ttgaaaaagc gaaagcagct ctggcataag ccagttgaaa gagggagcta
     4261 gtctccctct tttcgtttca acgccatcaa aacattgact tttatcgccg tagccttttc
     4321 aataaaggtc ttttgaagag taccaaaagg taacgcaagc aatgaatgct gctattttcc
     4381 gcttcttttt ttactttagc acctgaatcc aggaggctag cgcgtgagaa gagaaacgga
     4441 aaacagcgcc tgaaagcctc ccagtggagg ctttttttgt atgcgcgttt tgaaaaataa
     4501 aaagccagtc tatcaggcgt aattgtcgca gccagtctgg acacggccag cgcggagaaa
     4561 ccggagcgta tttaagtacg tgagaatttc gagcactgcc cgggaccaaa atggcaagta
     4621 aaatagcctg atgggatagg ctctaagtcc aacgaaccag tgtcaccact gacacaatga
     4681 ggaaaaccat gtcacatctc gcagaactgg ttgccagtgc gaaggcggcc attagccagg
     4741 cgtcagatgt tgccgcgtta gataatgtgc gcgtcgaata tttgggtaaa aaagggcact
     4801 taacccttca gatgacgacc ctgcgtgagc tgccgccaga agagcgtccg gcagctggtg
     4861 cggttatcaa cgaagcgaaa gagcaggttc agcaggcgct gaatgcgcgt aaagcggaac
     4921 tggaaagcgc agcgctgaat gcgcgtctgg cggcggaaac gattgatgtc tccctgctag
     4981 gtcgtcgcat tgaaaacggc ggtctgcatc cggttacccg taccatcgac cgtatcgaaa
     5041 gtttcttcgg tgagcttggc tttaccgtgg caaccgggcc ggaaatcgaa gacgattatc
     5101 ataacttcga tgctctgaac attcctggtc accacccggc gcgcgctgac cacgacactt
     5161 tctggtttga cactacccgc ctgctgcgta cccagacctc tggcgtacag atccgcacca
     5221 tgaaagccca gcagccaccg attcgtatca tcgcgcctgg ccgcgtttat cgtaacgact
     5281 acgaccagac tcacacgccg atgttccatc agatggaagg tctgattgtt gataccaaca
     5341 tcagctttac caacctgaaa ggcactctgc acgatttcct gcgtaacttc tttgaggaag
     5401 atttgcagat tcgcttccgt ccttcctact tcccgttcac cgagccttct gcagaagttg
     5461 acgtcatggg taaaaacggt aaatggctgg aagtgctggg ttgcgggatg gtgcatccga
     5521 acgtgctgcg taacgttggc atcgacccgg aagtttactc tggtttcgcc ttcgggatgg
     5581 ggatggagcg tctgactatg ttgcgttacg gcgtcaccga cctgcgttca ttcttcgaaa
     5641 acgatctgcg tttcctcaaa cagtttaaat aaggcaggaa tagattatga aattcagtga
     5701 actgtggtta cgcgaatggg tgaacccggc gattgatagc gatgcgctgg cgaatcaaat
     5761 cactatggcg ggcctggaag ttgacggtgt agaaccggtt gccggtagct tccacggcgt
     5821 ggtcgttggt gaagtggttg agtgtgcgca gcatccgaac gctgacaaac tgcgtgtgac
     5881 aaaagtgaat gtcggcggcg atcgcctgct ggacatcgtc tgtggtgcgc caaactgccg
     5941 tcagggcctg cgtgtggcgg tagctaccat tggtgctgtt ctgccgggtg atttcaaaat
     6001 taaagcggcg aaactgcgtg gcgaaccgtc tgaagggatg ctgtgctcct tctctgaact
     6061 gggaatttct gacgatcaca gcggcattat cgaactgcct gcagatgcgc cgattggcac
     6121 tgacatccgc gaatacctga aactcgatga caacaccatc gaaatcagcg taacgccaaa
     6181 ccgtgccgac tgcttaggta tcattggtgt tgcgcgtgac gttgccgtgc tgaatcagct
     6241 gccgctggtt gaaccggaaa tcgttccggt tggtgcgacc atcgacgaca cgttgccgat
     6301 tacagtcgaa gcgcctgaag cctgcccgcg ctatcttggc cgtgtggtaa aaggcattaa
     6361 cgttaaagcg ccaactccgc tgtggatgaa agaaaaactg cgtcgttgcg ggatccgttc
     6421 tatcgatgca gttgttgacg tcaccaacta tgtactgctc gaactgggcc agccgatgca
     6481 cgctttcgat aaagatcgca ttgaaggcgg cattgtggtg cggatggcaa aagagggcga
     6541 aacgctggta ctgcttgatg gcactgaagc gaagctgaat gctgacactc tggtcatcgc
     6601 cgaccacaac aaggcgctgg cgatgggcgg catctttggt ggcgaacact ctggcgtgaa
     6661 tgacgaaacg caaaacgtct tgctggaatg cgctttcttc agcccgctgt ctatcaccgg
     6721 tcgtgctcgt cgtcatggcc tgcatactga tgcgtctcac cgttatgagc gtggcgttga
     6781 tccggcactg cagcacaaag cgatggaacg tgcgacccgt ctgctgatcg acatctgcgg
     6841 tggtgaggct ggtccggtaa ttgatatcac caacgaagca acgctgccga agcgtgcaac
     6901 catcacttta cgtcgtagca aactggatcg cctgatcggc catcatattg ctgatgaaca
     6961 ggtaactgac attctgcgtc gtctcggctg cgaagtgacc gaaggcaaag acgagtggca
     7021 ggcagttgcg ccgagctggc gtttcgatat ggagattgaa gaagatctgg ttgaagaagt
     7081 cgcgcgtgtt tacggctaca acaacatccc ggatgagccg gtacaggcaa gcctgattat
     7141 gggtactcac cgtgaagctg acctgtcgct caagcgcgtg aaaacgctgc tcaacgacaa
     7201 aggctatcag gaagtgatca cctacagctt cgttgatccg aaagtgcagc agatgatcca
     7261 tccaggcgtt gaagccttac tgctgccaag cccgatctct gttgaaatgt cagcaatgcg
     7321 tctttctctg tggaccggcc tgctggcaac cgtggtgtac aaccagaacc gtcagcagaa
     7381 ccgtgtgcgc attttcgaaa gcggtctgcg tttcgtacca gatactcagg caccgttggg
     7441 cattcgtcag gatctgatgt tagccggtgt gatttgcggt aaccgttacg aagagcactg
     7501 gaacctggca aaagagaccg ttgatttcta tgatttgaaa ggcgatcttg aatccgttct
     7561 cgacctgacc ggtaaactga atgaggttga gttccgtgca gaagcgaatc cggcactgca
     7621 tccggggcaa tccgcagcga tttatctgaa aggggaacgt attggttttg ttggagttgt
     7681 tcatcctgaa ctggaacgta aactggatct taacggtcgc actctggtgt tcgaactgga
     7741 gtggaacaag ctcgcagacc gcgtggtgcc tcaggcgcgc gagatttctc gcttcccggc
     7801 gaaccgtcgt gacatcgcgg tggtggtcgc agaaaacgtt cccgcagcgg atattttatc
     7861 cgaatgtaag aaagttggcg taaatcaggt agttggcgta aacttatttg acgtgtaccg
     7921 cggtaagggt gttgcggagg ggtataagag cctcgccata agcctgatcc tgcaagatac
     7981 cagccgtaca ctcgaagaag aggagattgc cgctaccgtc gccaaatgtg tagaggcatt
     8041 aaaagagcga ttccaggcat cattgaggga ttgaacctat ggcgcttaca aaagctgaaa
     8101 tgtcagaata tctgtttgat aagcttgggc ttagcaagcg ggatgccaaa gaactggttg
     8161 aactgttttt cgaagagatc cgtcgcgctc tggaaaacgg cgaacaggtg aaactctctg
     8221 gttttggtaa cttcgatctg cgtgataaga atcaacgccc gggacgtaac ccgaaaacgg
     8281 gcgaggatat tcccattaca gcacggcgcg tggtgacctt cagacccggg cagaagttaa
     8341 aaagccgggt cgaaaacgct tcgcccaaag acgagtaatc tgatctaact aaaaaggccg
     8401 ctctgcggcc ttttttcttt tcactgtcga agagtcaccg taaaatcaac gccatgacac
     8461 ttcagcagaa cggataccat gctgacactt gcccgccaac aacagcgaca aaatattcgc
     8521 tggttattat gcctgtcagt tttgatgctg ctggcgcttc tcttaagcct ttgcgccggt
     8581 gaacaatgga tttcgccagg tgactggttt actcctcgtg gcgaactgtt cgtctggcaa
     8641 attcgcctgc cacgtacgct ggctgtattg ctggttggtg cggcgctggc tatatccggc
     8701 gctgtaatgc aggcgttgtt tgaaaatcct ctggcagaac ctggactact tggcgtctct
     8761 aacggcgcag gcgtggggct tatcgccgcg gtattgcttg ggcaagggca actccccaac
     8821 tgggcgctag ggctgtgtgc gattgctggc gcgcttatca tcactttaat actcttacgt
     8881 ttcgcccgtc gtcatctttc gaccagtcgg ttattgctgg ctggcgttgc attagggatt
     8941 atctgtagcg cactaatgac gtgggctatc tacttttcca cctctgttga tttacgtcag
     9001 ctgatgtact ggatgatggg cggttttggc ggcgtagact ggcggcaaag ctggctgatg
     9061 ctggcattga tccccgtgtt gttgtggatc tgttgtcagt ccaggccgat gaatatgtta
     9121 gcacttggcg agatctcggc gcggcaactg ggtttacccc tgtggttctg gcgcaatgtg
     9181 ctggtggcag cgaccggctg gatggttggc gtcagtgtgg cgctggcggg tgctatcggc
     9241 tttattggtc tggtgatccc acatattctt cggttgtgtg gtttaaccga tcatcgcgta
     9301 ttacttcccg gctgcgcgct ggcaggggcg agcgcagtgc tgctggccga tattgtagcg
     9361 cgcctggcat tagctgccgc agagctgcct attggcgtgg tcaccgcaac gttgggtgcg
     9421 ccggtgttta tctggttatt gttaaaagca ggacgttagc cgcaaaaaga cggtctatga
     9481 ttaaaagcta agatttttac tgaccacacc caggagaaac gatgcaagat tccattctga
     9541 cgaccgtagt gaaagatatc gacggtgaag tgaccacgct ggagaagtac gccggtaatg
     9601 tgctgttgat tgtcaatgtc gcctcaaagt gtggcttaac gccgcaatat gagcagttgg
     9661 agaatattca gaaagcctgg gccgatcgag gttttgtggt gctgggattc ccgtgcaacc
     9721 agtttctgga acaagaaccg ggcagcgatg aagagattaa aacttactgt accaccacat
     9781 ggggggtgac gttcccgatg ttcagtaaga ttgaagttaa cggcgaagga cgccatccgc
     9841 tgtatcaaaa attgattgcc gcagcgccga ccgcagtcgc gccggaagag agcggattct
     9901 atgcccgtat ggtcagcaaa ggtcgtgcac cgctgtaccc tgatgatatt ttatggaatt
     9961 ttgagaaatt tttagttggc agggacggat tggtcatcca gcgtttttcc ccggatatga
    10021 cgccggaaaa ccccattgtg atggaaagca ttaaactggc gttggcaaaa taatgtctat
    10081 tgtgatgcag ttacaggatg ttgcggaatc tacccgccta gggccacttt ctggcgaggt
    10141 tcgggctggg gagatcctgc acctggtggg gccgaatggc gcgggtaaga gtaccttact
    10201 ggcacgaatg gccggaatga ccagcggtaa gggaagtatt cagttcgcgg ggcaaccact
    10261 ggaagcatgg tccgcaacaa aactcgcgct gcatcgcgcc tatctttcac aacagcagac
    10321 gccgccgttt gcaatgccgg tctggcacta tctgacactg catcagcacg ataaaacgcg
    10381 taccgaacta ctgaatgatg tcgcaggggc gctggctctt gatgacaaac tcggacgtag
    10441 caccaatcaa ctttccggcg gtgaatggca acgcgtacgt cttgctgcgg tggtgctgca
    10501 aatcacacca caagccaatc ccgcaggcca attgctgctt cttgatgagc cgatgaacag
    10561 tcttgatgtt gcgcaacaaa gtgcgttaga caaaattctg agcgctctgt gtcagcaagg
    10621 actggcgatt gtgatgagca gtcacgatct caaccacaca ttgcgtcatg cgcatcgggc
    10681 gtggttgcta aaaggtggaa aaatgctggc cagtggacgc agagaagagg tgctcacgcc
    10741 gccaaatctg gcgcaggcct atgggatgaa ttttcgccgt ctggatatcg aaggtcacag
    10801 aatgctgatt tcgaccatct gatttcatta gtatcttgaa aatcgggtaa ttacgacgct
    10861 aaattaatgc aagaataaaa aacagaggaa ccgtacggaa tgcgtttctg ccttattttg
    10921 atcacagcac tgtttctggc cgggtgtagc caccataaag caccgccgcc aaatgccaga
    10981 ctttctgatt cgattaccgt tattgccggt ttgaacgacc agctacaaag ctggcatggc
    11041 acgccgtatc gttatggtgg catgacgcgg cgcggtgtgg actgttcggg atttgtggtt
    11101 gtaacgatgc gcgatcgttt cgatttgcag ctgccccgag aaaccaaaga acaagcctct
    11161 atcggcacgc aaattgataa agacgagttg ctgcctggtg acctggtctt tttcaaaacg
    11221 ggttccggac aaaatggttt gcatgtaggt atttacgata ccaacaacca atttatccac
    11281 gcctctacca gcaagggagt gatgcgttcc tcacttgata atgtctattg gcagaaaaat
    11341 ttctggcagg cgagacg
//
LOCUS       DAJVQG010000125        11329 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJVQG010000125 DAJVQG010000000
VERSION     DAJVQG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11329
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.125"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            460..1386
                     /locus_tag="OQM83_003069"
     CDS             460..1386
                     /locus_tag="OQM83_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1521270.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFERAEQTPGIKADWLV
                     RMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQARTLPDMLSSDAG
                     PLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(1336..2529)
                     /locus_tag="OQM83_003070"
     CDS             complement(1336..2529)
                     /locus_tag="OQM83_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1521271.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2665..4389
                     /gene="iucA"
                     /locus_tag="OQM83_003071"
     CDS             2665..4389
                     /gene="iucA"
                     /locus_tag="OQM83_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCS1521272.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4390..5337
                     /gene="iucB"
                     /locus_tag="OQM83_003072"
     CDS             4390..5337
                     /gene="iucB"
                     /locus_tag="OQM83_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCS1521273.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5337..7079
                     /gene="iucC"
                     /locus_tag="OQM83_003073"
     CDS             5337..7079
                     /gene="iucC"
                     /locus_tag="OQM83_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCS1521274.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            7076..8413
                     /gene="iucD"
                     /locus_tag="OQM83_003074"
     CDS             7076..8413
                     /gene="iucD"
                     /locus_tag="OQM83_003074"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCS1521275.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8419..10614
                     /gene="iutA"
                     /locus_tag="OQM83_003075"
     CDS             8419..10614
                     /gene="iutA"
                     /locus_tag="OQM83_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCS1521276.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(11165..11308)
                     /locus_tag="OQM83_003076"
     CDS             complement(11165..11308)
                     /locus_tag="OQM83_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521277.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2844 a         2860 c         2926 g         2699 t
ORIGIN      
        1 ctcaacgaag gggccgacaa acacgttgtc cctgagttca cattcataaa tgttgaccgg
       61 tttgacaata cggacattga tcccataaat cacatcccgg acagcacatt ccagaaattt
      121 catactcaca cgtcctttaa atacagatga catttgccgg aattataccg cccagattca
      181 cttgccatgt ttcagaatag cttataagcc gcacttttac cgatactcag caacaaatca
      241 ggtctgtatc cacttcagcc ggaaaccgga tctgcgcggc ggggaacggg caagaaatat
      301 gataattaag caacacacat aaaatcatat tgcataatcg catctgaatc gaacacttat
      361 tcgcatcact atcgatcatc acttcgcatc tcataccctt acccacaagt tgaaataata
      421 gtattatatt gaaaagacag agattatttc agaggtgtta tggaaaactg ggttattcag
      481 gagctaaaat cactggatat aggggactca agacttgata aaagagtaaa acatatcctc
      541 aatagcctca gtcgttcacc agaagaaagt attcctgttt cctgccgtac ctggagtgaa
      601 acaaaggcag cttatcgttg tttttcaaac gataaagtct ctgctaataa aattatggct
      661 cctcacaaag aaaatatcac tgaaagagct cagcagttta aaagagtttt agttttacag
      721 gatacaacag aacttaatta ttctggtcag aaagaaaaac aaggagttgg tcacaaaaaa
      781 cataaagatg aaagaaactt atttcttcat cctcaactcg tcatcagtga gtcaggtgtt
      841 tgcctcggtg tttatgatga ctatcagtgg ttcagggatg aattaaaaac cagtaaaaat
      901 acccgacaag aaatctgcaa tgatttactt cataaaaaac atgtcagtga aaaagaaacc
      961 tggcgttggg ttgaaggata taataaagca acagaactgg ctcgccaatg ccctgatacc
     1021 catgtattgt caatttctga tcgtgaaggc gatttttatg acctgtttga acgcgcagag
     1081 cagactcccg gaataaaagc tgactggctt gtcagaatga aatttaataa cagagcaacg
     1141 ctgaatatta atggtaaacg ggatcatagg ttgttgcacg aaagaatcat ggagattaca
     1201 ccgcagcaac tggttgaatt cactatacct gatgggcggg ggcagcaagc cagaacactg
     1261 cctgacatgc tttcatcaga cgcaggtcct ctctcatatc ctctgattta ctccagtaag
     1321 gtgatgatca tatctttatt tgatagtacg gtaatgccag ataaatacag acacaatggt
     1381 aaataatata gttgcaaata atgctgccat aatcagcgcg attgcgacac ctgttttcga
     1441 taccagcggc accagcaaag cgcacagtcc atagccaagt gtgtgactgg tagcgagcca
     1501 gcccgcgcct gcgccatcag ccagcttatc attcagtaga agttgataag cgggtgttgc
     1561 cagagctgct ccaaatgaca gcactgcaca gcccgggtaa aacaaccata gctgttcgga
     1621 gagcatgata gccagaccac cactcatcaa cactccggca ctcaggagca gggccaccgg
     1681 agtcaggcgc tgcggacgga gtaccccgaa ctgcgcgata agcgcagcta ctgcggacag
     1741 acccaacagc caggccacct gttggctaat ggcggtggta tccgtgacga actggcgagt
     1801 aagggcaggc gaaagtccaa gctgcatcat gctcaccgcc gcagccagta atattgcgca
     1861 aagcagataa ggcagacaat cccgcttcag actgacgctc ttgcactccg gtgtgggctg
     1921 tggtggtgtg ccgggcaacc gcagcagcat cagcagcgcc agcactggtg ctgccatcag
     1981 tagccccagt ggcgccagag gatgaatggc cagcattgct gccgcgcaca gcggcccgaa
     2041 tagcctgccg caactcaggc cggagctgat ggttgccaga gcggccatgc gattcccttc
     2101 tccagctctc tgcaatgccc agacctgaca ggctggcacc attgccgaca cggtcagccc
     2161 gtaggcgatc cgggcgatga ttaatattcc cagtcccacc gctgttgtta tggcgctggt
     2221 agccatcagc acgcttccca gccctagcag ggtaaagctt atgccatatc ctcccagagc
     2281 ccacaacacc acaggtttac tgccagtacg ggaaatttgc tttccccacc acggggacga
     2341 tggcagaaac agcattgagc caagcatcag cagggcagcc catacagaca gactcagatt
     2401 tgtctgtatg accagaacag ggagcacaac cagcagaccg ttctgtccga taccgagaag
     2461 cccggcactg aacgcaagtg gccagcagga cagtgttttt tggggcgtat cttcgatccc
     2521 tggtgacatt ataatgtttc aactccatgt attaattgtg tttatttgta aaattaattt
     2581 atctgacaat aacatttatt gttgataatg agaatcatta tcgacataat tgttattatt
     2641 ttactgtgtg ggagctgttt gactatgacc ctgccctctg aaaaaccagc cacagatgtg
     2701 gctgcgcagt gcttcctgaa tgcactgatt cgtgaaacca cagactggaa actgacagaa
     2761 tacccgccag acgaattgct catcccgctg gatgagcaga aatcgctcca tttcagagtg
     2821 gcttatttct ccccaaccca gcatcaccgc tttgcatttc ctgcacgtct ggtcacggca
     2881 tcaggcagtt atcctgtcga ctttaccact ctctcccggc ttattattga taagctacgc
     2941 catcaactgt ttctgcccgt tcccctctgc gaaactttcc accagcgcgt gctggaaagc
     3001 catgtccata cgcaacaggc aattgatgcc cgtcatgact gggccgctct gcgtgaaaaa
     3061 gcgttgaatt ttggcgaggc tgagcaggcg ctgctgacag gacacgcttt ccaccctgcg
     3121 cctaagtctc atgaaccgtt taaccgacgg gaggctgaac gctacctgcc tgatatggca
     3181 ccccacttcc cactgcggtg gttttcggtg gataaaacgc aaatcgccgg tgaaagtttg
     3241 catctcaacc ttcaacagcg gctgacgcga tttgccgcag agaatgcgcc tcagttactc
     3301 aacgaattaa gtgacaacca atggctgttc ccgttgcacc cgtggcaggg ggaatatctt
     3361 ttgcagcagg ggtggtgcca ggcacttgtt gctaaagggc tgattaaaga cttaggtgag
     3421 gccggcacgt cgtggctgcc gaccacctct tcccgttccc tctactgtgc caccagccgc
     3481 gatatgatca agttctccct gagcgtacgg ctgaccaact ccatccgtac tctgtccgtg
     3541 aaagaagtga agcgtgggat gcgcctggca cgcctggctc aaaccgacgg ctggcagatg
     3601 ctacaggtcc gcttcccgac tttccgggta atgcaggagg atggctgggc cgggctgctc
     3661 gatcttaacg gcaacatcat gcaggaaagt ctgtttgccc tgcgtgaaaa tctgctggtg
     3721 gatcagccga aaagccagac taacgtactg gtctccctga ctcaggccgc acctgatggc
     3781 ggtgattcgc tgctggtttc ggcggtaaaa cgcctgagcg atcgcctcgg tatcactgtg
     3841 caacaggccg cccatgcatg ggtcgatgca tactgtcagc aagtgctaaa gccgctgttt
     3901 acggctgaag cggattacgg cctggtgctg ctggcgcatc agcaaaatat tcttgtccag
     3961 atgcttgggg atctgccggt cggatttatt taccgtgact gtcagggcag cgcttttatg
     4021 cctcacgcga cagactggct cgattctatt ggcgaggcgc aggcggaaaa tattttcacc
     4081 catgagcagt tgctgcgcta tttcccttat tacctgctgg ttaactccac ttttgctgtg
     4141 accgctgcgc tgggggctgc cgggctggac agcgaatcga atctgatggc tcgtgtacga
     4201 gcatcgctgg ctgaagtgcg tgatcaggtg actcataaaa cctgtctcaa ctacgtgctg
     4261 gaaagtccgt actggaacgt aaaaggtaac tttttctgtt atctgaacga tcataacgag
     4321 aacaccattg ttgacccttc ggtgatctat ttcgatttcg ctaacccgct gcaggctcag
     4381 gaggtctgaa tgtctgaggc aaacattatt cacagcagat atggactgcg ctgtgaaaaa
     4441 ctcgacaagc ccctgaatct tggctgggga ctggacaata gcgcggtgtt gcactgtccc
     4501 ggggagctgc cgacagggtg gctgtgcgat gcgctggatc agatatttat cgccgcacca
     4561 caactgtcag cagtggctct cccctgggcc gaatggcgtg aggagccaca ggcgctgacg
     4621 ctttttgggc aggtaaaaag cgacattatc catcgtactg ccttctggca gttaccgtta
     4681 tggttgagtt caccggcaaa ccgggcctcc ggtgaaatgg tttttgatgc agagcgtgag
     4741 atttatttcc cgcagcgccc accccgtcca cagggtgagg tttaccgccg ttacgatcca
     4801 cgtattcgca ggatgctgag tttccgcatt gccgatcccg tttctgatgc agaacgtttc
     4861 acccgctgga tgaatgatcc gcgcgttgag tatttctggg agcaaagtgg ctcactggag
     4921 gtacagaccg cctatctgga gcgccagctc accggtaaac atgcattccc gctgattggg
     4981 tgcttcgacg atcggccatt cagctatttc gaaatctact gggcggcgga agaccgcatt
     5041 ggtcgtcact attcgtggca gccctttgac cgtggcctgc atctgctggt tggtgaacag
     5101 caatggcgcg gggcccacta tgtgcaaagc tggctgcgcg ggctgacaca ttacctgctg
     5161 ctggatgagc cccgtacgca gcgcacagta ctggagccac gcaccgataa ccagcgcctg
     5221 ttccgccatc ttgagcctgc gggataccgg acaattaaag agttcgactt cccacataag
     5281 cgctcgcgca tggtgatggc ggatcgtcat cacttcttca cggaggtcgg tctgtaatga
     5341 atcacaagga ctgggatttt gttaaccgtc ggctggtggc aaaaatgttg tctgagatgg
     5401 agtatgagca ggttttccac gcggaatctc agggcgatga ccactactgc attaacctgc
     5461 cgggagcaca atggcgcttc atcgctgaac gtggtatctg gggctggctg tggattgatg
     5521 cccaaacgtt gcgctgcacg gacgaaccag tactggcgca gacgctgctg atgcagctaa
     5581 agccggtgct gtcaatgagt gatgccactg ttgcagagca tatgcaggat ttgtatgcca
     5641 cgctactggg cgacctgcaa ctgctgaagg cccgtcgcgg actgagtgcc agtgacctga
     5701 ttaatcttga tgccgatcgt ctgcaatgcc tgctgagcgg tcatcctaaa ttcgttttta
     5761 ataaaggtcg ccgtggctgg ggcaaagagg cgctggaacg atatgcgcca gagtatacca
     5821 acaccttcag actgcactgg ctggcggtaa aacgtgaaca tatgatctgg cgctgcgata
     5881 acgatctgga tattcagcag ttgttgactg ccgcaatgga tccgcaggag tttacacgct
     5941 tcagtcaggt ctggcaggaa aacgggctgg atcacaactg gctaccgctg ccggtacatc
     6001 cgtggcagtg gcagcaaaaa atcgccacag acttcatcgc tgattttgtc gaaggcagga
     6061 tggtgtctct cggcgagttt ggcgaccagt ggctcgccca gcagtcgctg cgtaccctga
     6121 ccaacgccag ccgccgggga gggctggata tcaagctgcc gctgaccatc tacaacacct
     6181 catgctaccg gggaatccct ggcagatata tcgctgccgg accactggct tcacgctggc
     6241 tgcaacaggt ttttgcgacc gacgccaccc tggtgcaaag cggcgcagtg atccttggtg
     6301 aaccggctgc aggctatgtg tcccatgaag gctatgccgc gcttgcccgg gctccctatc
     6361 gctaccagga aatgcttggt gttatctggc gggagaatcc gtgccgctgg ctgaaaccgg
     6421 atgaaagtcc tgttctgatg gcaacactga tggagtgcga cgaaaacaat cagccgctgg
     6481 caggcgcata tatcgaccgc tccggactgg acgctgaaac ctggcttacg caattgttcc
     6541 gggtggttgt ggttccgctg tatcacctgc tttgccgcta cggtgtcgcg cttattgcac
     6601 acggacaaaa tataacgctc gccatgaaag agggggtacc acagcgtgtt ctgctgaaag
     6661 acttccaggg cgatatgcgg ctggtgaaag aagcgtttcc cgaaatggac tctttgcctc
     6721 aggaggtgcg tgatgccaca tcccgtctga gtgcggacta cttaatccat gatttgcaga
     6781 cgggtcactt cgtgacagta ctgcgtttta tttcgccact gatggttcgt cttggcgtac
     6841 ctgaaaggcg attttatcaa ctgctggcag cagtgttgag tgattacatg aacaaacatc
     6901 cacaaatggc agagcgtttt gcgcttttct cactcttcag gccacaaatc attcgcgtgg
     6961 tgctgaaccc ggtaaaactg acctggccgg acctggatgg cggcagtcgc atgctgccga
     7021 attaccttga gaatctgcaa aatccgctgt ggctggtaac tcaggaatat gaatcatgaa
     7081 aaaaagtgtc gattttattg gtgtcgggac agggccattt aatctcagca ttgctgcatt
     7141 gtcacatcag atcgaagaac tgaactgtct cttctttgac gaacatcctc atttttcctg
     7201 gcatccgggt atgctggtac cggattgtca tatgcagacc gtctttctga aagatctggt
     7261 cagtgcagtt gcacctacaa atccctacag ctttgttaac tatctggtga agcacaaaaa
     7321 gttctatcgc ttccttacaa gcaggctacg tacagtatcc cgtgaagagt tttctgacta
     7381 tctccgctgg gctgctgaag atatgaataa cctgtatttc agtcataccg ttgaaaatat
     7441 tgatttcgac aaaaaaagtc gattgtttct ggttcagacc agtcggggag aatattttgc
     7501 ccgcaatatc tgccttggta caggaaaaca accttattta ccaccctgtg tgaagcatgt
     7561 gacacaatcc tgtttccatg ccagtgaaat gaatcttcgt cggccggacc tgagtggaaa
     7621 acggataacc gtggttggtg gaggacagag tggtgcagac ctgttcctta atgcattacg
     7681 cggggaatgg ggagaagcgg cggaaataaa ctgggtctca cggcgtaata attttaacgc
     7741 actggatgag gctgcttttg ctgatgagta ttttacacct gaatatattt caggcttctc
     7801 cggactgaag gaagatattc gccatcagtt actggatgag cagaaaatga catcggatgg
     7861 catcactgcc gattctttac tgaccattta tcgtgagttg taccaccgtt ttgaagttct
     7921 gagaaaacca agaaatatcc gtctgctacc cagtcgctcg gtaacaaccc tggaaagtag
     7981 tggtccgggc tggaggttat tgatggaaca tcatctggat cagggcagag agagcctgga
     8041 aagtgatgtg gtgattttcg ccacaggtta ccgttctgca ttgccacaaa tacttccctc
     8101 actgatgccc ttgatcacca tgcacgataa gaacaccttt aaagtgcgtg atgacttcac
     8161 tctggaatgg agtggcccga aagaaaataa catctttgcg gtcaacgcca gcatgcaaac
     8221 tcatggcatc gccgaacccc agctcagcct gatggcctgg cgatctgcac gtattcttaa
     8281 tcgcgtactg gggcgtgatt tattcgatct cagtatgccg cccgcactga ttcagtggcg
     8341 cagcggcagc cggaaaaaac cgcagccgga ggctgcttct ttaactcact atacagcaaa
     8401 tattcaggaa taacgatgat gcgcaaaaag tatatgcccc gggctcttgg tccgctgctt
     8461 cttgtcgtgc tgtcaccagc tgtcgcccag caaaacgatg ataatgagat catagtgtct
     8521 gccagccgca gcaatcgaac tgtagcggag atggcgcaaa ccacctgggt tatcgaaaat
     8581 gccgaactgg agcagcagat tcagggcggt aaagagctga aagacgcact ggctcagtta
     8641 atccccggcc ttgatgtcag cagccagagt cgaaccaact acggtatgaa catgcgtggc
     8701 cgcccgctgg ttgtcctgat tgacggtgtg cgcctcaact cttcacgttc cgacagccga
     8761 caactggact ctgtcgatcc ttttaatatc gaccatattg aagtgatctc cggcgcgacg
     8821 gccctgtacg gtggcgggag taccggaggg ttgatcaaca tcgtgaccaa aaaaggtcag
     8881 ccggaaacca tgatggagtt tgaggctggc acaaaaagtg gctttaacag cagtaaagat
     8941 cacgatgagc gcattgccgg tgctgtctcc ggcggaaatg accatatctc cggacgtctt
     9001 tccgtggcat atcagaaatt tggcggctgg tttgacggta acggcgatgc caccctgctt
     9061 gataacaccc agaccggcct gcagcactcc aatcggctgg acatcatggg aaccggtacg
     9121 ctgaacatcg atgaatcccg gcagcttcaa ctgataacgc agtactataa aagtcaggga
     9181 gacgacaatt acgggcttaa tctcgggaaa ggcttttccg ccatcagcgg gagcagcaca
     9241 ccatacgtca gtaaggggct gaattctgac cgcattcccg gcactgagcg gcatttgatc
     9301 agcctgcagt actctgacag tgatttcctg ggacaggaac tggtcggtca ggtttactac
     9361 cgcgatgagt cgttgcggta ctacccgttc ccgacggtaa atgcgaataa acaggcgaca
     9421 gctttctcct cgtcacagca ggataccgac cagtacggca tgaaactgac tctgaacagc
     9481 caactgatgg acggctggca aatcacctgg gggctggatg ctgagcatga gcgctttacc
     9541 tccaaccaga tgttcttcga tctggctcag gcaagtgctt ccggagggct gaacaaccat
     9601 aagatttaca ccaccgggcg ctatccgtca tatgacatca ccaatctggc ggccttcctg
     9661 caatccagct atgacattaa tgatattttt accgttagcg gtggcgtacg ctatcagtat
     9721 actgagaaca gggtagatga tttcatcgac tacacgcagc aacagaagat tgctgccggg
     9781 aaggcgatat ctgccgacgc cattcctggt ggttcggtag attacgataa ctttctgttc
     9841 aatgctggtc tgctgatgca catcaccgaa cgtcagcagg catggttcaa tttttcccag
     9901 ggggtggcat tgccggatcc ggggaaatat tatggtcgcg gcatctatgg tgcagcagtg
     9961 aacggccatc tttccctgac aaagagcgtg aacgtcagcg acagtaagct ggaaggcgtg
    10021 aaagtcgatt cttatgaact gggctggcgc tttaccggtg acaacctgcg gactcaaatc
    10081 gcggcatatt actcgctttc caataagagc gtggaaagga ataaagatct gaccatcagt
    10141 gtgaaggacg acaggcgccg tatttacggc gtggaaggtg cggtggacta cctgatcccg
    10201 gatactgact ggagtaccgg tgtgaacttc aatgtgctga aaaccgagtc gaaagtgaac
    10261 ggtcaatggc aaaaatatga cgtgaaggaa tcaagtccat cgaaagcgac agcttacatt
    10321 aactgggcgc cggaaccgtg gagtctgcgt gtacagagca ccacttcttt cgacgtaagc
    10381 gatgcagagg gtaacgatat taatggttac actaccgtcg attttatcag tagttggcag
    10441 cttccggtgg gaacactcag cttcagcgtt gagaacctct tcgaccgtga ctataccact
    10501 gtctggggac agcgtgcacc tctgtactac tgcccgggtt acggccctgc ttcactgtac
    10561 gactacaaag gccggggccg aacctttggt ctgaactact cagtgctgtt ctgacgggga
    10621 tgcttttgca acaagggtat gctgtaaaca acatggtcct tggtggccat gttgtttaca
    10681 gccagtataa tatacagcct taacaagata ttccgtctcc ggaatggact gttcaccact
    10741 gaataacatt atttctgttt tatggatctt tgactgaaat ttatcgcata tcctcatttc
    10801 attgtccgtc tgaaaactaa ccttacaagt aagcgtcgtc tcagcaccgt ctggcagatc
    10861 ctgaaattcc tgagaggatg atctgcgccc aatatcctga gccggaactt tgctgcaatg
    10921 aaggccaacg aaaaatgggg gagtatgtta tagagtactt gctacagggt aaaaagaggt
    10981 acctgtcgct ggttctccgt tcagtttaac cgggagattt tctcctgcgc ctgtcataaa
    11041 gtccggtaat ggagattgtt aatacgatgg tgcacgatgc attgtcggtg cccggcccgg
    11101 aggatgcccc gtaactacat acggatcagg gttggcaata tcgaatggca ggctataggc
    11161 aaagttaaag ccccatggag tagcacaaaa tatgccacac aaaggaaacg gtctgaataa
    11221 cgcagtgatg gagaacttct tcggcacact gcaaaatcgc atcgtggtcg atcgctaatt
    11281 cgccgtgaat cactgaacga tgttccatgc gaacagatat tcgatcata
//
LOCUS       DAJVQG010000126        11269 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJVQG010000126 DAJVQG010000000
VERSION     DAJVQG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11269
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.126"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..226)
                     /gene="bamE"
                     /locus_tag="OQM83_003077"
     CDS             complement(<1..226)
                     /gene="bamE"
                     /locus_tag="OQM83_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCS1521278.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFY"
     gene            complement(375..2036)
                     /gene="recN"
                     /locus_tag="OQM83_003078"
     CDS             complement(375..2036)
                     /gene="recN"
                     /locus_tag="OQM83_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCS1521279.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2122..3000)
                     /gene="nadK"
                     /locus_tag="OQM83_003079"
     CDS             complement(2122..3000)
                     /gene="nadK"
                     /locus_tag="OQM83_003079"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCS1521280.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3123..3716
                     /gene="grpE"
                     /locus_tag="OQM83_003080"
     CDS             3123..3716
                     /gene="grpE"
                     /locus_tag="OQM83_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCS1521281.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3771..5057)
                     /locus_tag="OQM83_003081"
     CDS             complement(3771..5057)
                     /locus_tag="OQM83_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCS1521282.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5078..5869)
                     /locus_tag="OQM83_003082"
     CDS             complement(5078..5869)
                     /locus_tag="OQM83_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCS1521283.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6036..7397
                     /gene="ffh"
                     /locus_tag="OQM83_003083"
     CDS             6036..7397
                     /gene="ffh"
                     /locus_tag="OQM83_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCS1521284.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7646..7894
                     /gene="rpsP"
                     /locus_tag="OQM83_003084"
     CDS             7646..7894
                     /gene="rpsP"
                     /locus_tag="OQM83_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCS1521285.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            7913..8461
                     /gene="rimM"
                     /locus_tag="OQM83_003085"
     CDS             7913..8461
                     /gene="rimM"
                     /locus_tag="OQM83_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCS1521286.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8492..9259
                     /gene="trmD"
                     /locus_tag="OQM83_003086"
     CDS             8492..9259
                     /gene="trmD"
                     /locus_tag="OQM83_003086"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCS1521287.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9301..9648
                     /gene="rplS"
                     /locus_tag="OQM83_003087"
     CDS             9301..9648
                     /gene="rplS"
                     /locus_tag="OQM83_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCS1521288.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9725..10207)
                     /locus_tag="OQM83_003088"
     CDS             complement(9725..10207)
                     /locus_tag="OQM83_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCS1521289.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10223..>11269)
                     /gene="dgcN"
                     /locus_tag="OQM83_003089"
     CDS             complement(10223..>11269)
                     /gene="dgcN"
                     /locus_tag="OQM83_003089"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HCS1521290.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2765 a         2711 c         3027 g         2766 t
ORIGIN      
        1 catagaacca ggtattcgta ccaaatggat cggacatcag cggtgtaccc aatgcgtacg
       61 caacttgttg ttgcgtcatg ccaacacgta ttttggatac gtcgttagcg gtcagatagt
      121 tcccctggtt gatgtcagga cggtaaacca ctcgctccag agtggaacag cctgcggtca
      181 acatcaatag tactgctgct gcagcagtca gcgttttaca gcgcatagtg atttgattcc
      241 ttttcgggcc cgagcagtac gtggctcatc tgtaatatgc cgatgataat agacctttca
      301 tcactttaaa accttttgct ttccggtctt acggcgtttt gctgtttact ctaaccgtga
      361 agcaggaaaa aagtttacgc tgcaagcagt tctttcgcat tcgctagtgt attacgtgtg
      421 acttcactgc caccaagcag gcgcgccagc tcttgtaacc gcgctttttt attcagggat
      481 tgcatatgcg tttctgtcat cgcaccatcg gtttctttgc tgacaaaata gtgttgatga
      541 ccacatcccg cgacttgtgg caggtgggta acacacatca cctgagttga ttcgccgagt
      601 tgacgcagca gtttgccgac aactgccgct gttggaccgc taatccctac atccacttca
      661 tcaaaaatca gtgccggggt ttccatttta cgcgccgtga tgacctggat tgccagtgcg
      721 atgcgggaca attcaccacc ggatgcgact ttggcaatag gctgcattgg ctgacctggg
      781 ttggtggtta cccgaaactc aatacgatcg gcaccgtcag cgcccaggtg atgctcgtca
      841 aatttaacat cgatcgtaaa ctgcccatgc ggcattgaga gcgcatgcat actgtcggtg
      901 atcagctgtg caagttcttc tgcatattgc tggcgttgtt ggtgtaatgc gcgcgcgatt
      961 tccagtgcct gctgatgatg tttcgttacc gccagcgcaa gcgtttcttg tgagtcggcc
     1021 tgatcgtcca gttgctgctg ttcttccagt agcgactggt aatactgtgg caatgcctca
     1081 gggctgacgt gatgtttacg tgccagcgaa atctgttttg agatgcgctg ttcaagttca
     1141 aatagtcggt tgggatcgag atccagacga tcgcagtagt ggcgcagttc atcgctggct
     1201 tcagcaatct ggatggtagc ttcttccagc atatcaagta cgccggacag tttgctgtcc
     1261 atgccaatca attcgctcac cagttgttta gccgtgtaaa gctgactttg caggtttgcg
     1321 tcttcaccgt cggccattaa tgccaatgca ttctggctgg tggtcagcaa ttgaccgctg
     1381 ttcgccagac gtttgtactc ttcgtcgatt tgttcaaact ctccgggctg cggattaaat
     1441 tcgttaagtt cttttaattg gtattgcagc agttccgcac gggcggcgcg ttcctgactt
     1501 aactgttgat gatgcgcgag gtcacggcag ctttgatgcc acaactgata acgtgcggtc
     1561 atttcctgca gtagagaggt ttcattggca tagccatcaa gcaggaattt ttggtgctca
     1621 ggtttggtga gtaattgatg agcgtgctga ccatggatct gaatcagcaa ctgacccagt
     1681 tcgcgcagtt gtgacagagg aacagctgta ccgttgatga aaccacggga gcgaccatcg
     1741 ctgctgatca cgcgacgaag caaacattca tgcccgtctt caagctggtt ttcttccagc
     1801 cagcgcagag ccgctggcgt atctttcaga gaaaaacggg cgcacaggtc agcgcgagca
     1861 gcgccggtac gcaccatgtc ggcttcagcg cgaccaccga gacaaagacc gagggcatct
     1921 attgcaatag atttacccgc gccggtctcg ccagttatta cggtcatgcc gctatgaaaa
     1981 tcaatctcaa gctcacgaac gatagcaaag ttgctgatgg tcagttgtgc caacatagtc
     2041 gttttcctgt atgaaaaacc attactgtta ttgtgtacag tataaactgg ttttatatac
     2101 agtaaagagg ctggcgtaaa attagaataa tttttttgac cagccgagct tggtgcttaa
     2161 tgtgttgaaa taactgtaat ctttcggatg aatcagattc agatggtaat cacagcgacg
     2221 aatcaggaca tcttcacctt cctgaatcgg cagtgctatc tggctgtcgc aactgatttc
     2281 caggtcgtta cggcgatgcg aaaaacgcag acggatcgtg ctgctgctgt ttatgaccag
     2341 tggtcgtgct gacaacgtat gcgggaacat gggcaccagg gtaatcgcat ccagagaggg
     2401 ggtcagaata ggaccgcctg cagagaggga ataggcggtg gagcctgttg gcgtcgaaat
     2461 aattagtcca tcagatcgct gagaaaacgc aaagatctcg tcgatataca cttcgaactc
     2521 aatcatatgc gccactttgc ctggatgaag caccacttca tttatcgcgg tgctgatgcg
     2581 tttctggcaa tcttgctgac agacttgcgc ttccagcaaa aaacgtttct cgctgatgta
     2641 gtggccttcc agcacatcgg ctaactgttg ctgggcgtta tcggggtcaa ggtcagtcag
     2701 gaaacccagg ttgccacggt tgattccaat aactttaata tcgtaacggg cgagtgtgcg
     2761 tgccgcgccc agcatattac cgtcgccacc aacgactacc gcgagatcag ctagttgccc
     2821 aatctccgcg agcgtgccag ttttcacatt cttcagttgc agttcgtgag cgatttgttg
     2881 ctcaacgatg acctcgtaac cttttgtgca cagccagcgg tagagcattt catgtgttgt
     2941 cagtgcagtg gggtgccgtg ggtgtcccac aatgccaata cacttgaaat gattattcat
     3001 ttttccgagg tccttgttgc gaagattgat gacaatgtga gtgcttccct tgaaaccctg
     3061 aaactgatcc ccataataag cgaagttagc gagatgaatg cgaaaaaaac gcggagaaat
     3121 tcatgagtag taaagaacag aaaacgcctg aggggcaagc cccggaagaa attatcatgg
     3181 atcagcacga agagattgag gcagttgagc cagaagcttc tgctgagcag gtggatccgc
     3241 gcgatgaaaa aattgcgaat ctcgaagctc agctggctga agcccagacc cgtgaacgtg
     3301 acggcatttt gcgtgtaaaa gccgaaatgg aaaacctgcg tcgacgtact gaactggata
     3361 ttgaaaaagc ccacaaattc gcgctggaga aattcatcaa cgaattgctg ccggtgattg
     3421 atagcctgga tcgtgcgctg gaagtggctg ataaagctaa cccggatatg tctgcgatgg
     3481 ttgaaggcat tgagctgacg ctgaagtcga tgctggatgt tgtgcgtaag tttggtgttg
     3541 aagtgatcgc cgaaactaac gtgccgctgg acccgaatgt gcatcaggct atcgcaatgg
     3601 tggaatctga tgacgttgcg ccaggtaacg tactgggcat tatgcagaag ggttatacgc
     3661 tgaatggtcg tacgattcgt gcggcgatgg taactgtagc gaaagcaaaa gcgtaatttc
     3721 tgttttcgta ataattcacg gccctgcatg cgaatgcagg gccgttttcg ttactccgcc
     3781 acactctcgc gcagcggttt cacaggaaaa acttttacct gcttaatcat attgtcctgt
     3841 acgtcgagaa tatctatatc gtactcgcca atacgcacgc gggtgcctgc gacagggatc
     3901 tcttccagtg cctcaagaat gacgccatta accgtgcggg catcatcttc cggtagatgc
     3961 cagttaaagg ctttgttgat ttcccgcacg ttggcggtgc catcgataat caccgaaccg
     4021 tcgttttgcg gcgtgacctc ttcggcaagt gttggcgaca tcgacgtagt gaaatcgccg
     4081 acaatctctt ccagaatatc ttcaaccgtc accagcccct gaatgtctcc atactcgttg
     4141 acgaccaggc cgactttctt tttgttgcgc tgaaacttta ccaactgcgt gctgagcggc
     4201 gtaccttccg gcacaaaata gatctcgtcc gcggcgcgca gcatggtttc tttggtgaac
     4261 tcttttttct ccgacatcaa ccgccaggct tcacgtactc gcagcatact gatggcgtcg
     4321 tccagcgaat cacggtagag cacgatgcgc ccgtgaggtg agtgggagag ttggcgcaga
     4381 atcgatttcc agtcatcgtt gatatcaata ccgataattt cactgcgcgg caccatgatg
     4441 tcatcaacgg tcattttttc cagatcgagc accgacagca gcatatcctg attgcgacgg
     4501 gaaatttgtg agcgtgattc gtgcacgata gtgcgcaact cttctttgct caaagagccg
     4561 ctaaccacga tatcggtttt gatacccatc atgcgcatca gcatacgggt gatagcattc
     4621 agcaaccaga ccagcggcat catcaaaatt tgcagcggag ccagcagaaa actactcgga
     4681 taagcgactt tttccgggta cagcgcggca atggttttcg gcaatacctc agcaaatacc
     4741 agtacgacaa aagtcagcac accagtcgca attgccacgc ccgcatcgcc gtacaaacgc
     4801 atcccaacaa tagtgccgag cgcggaggca agaatattga ccaggttatt gccgattaac
     4861 accaggctta tcaggcggtc tggcttacgc agcaattttt cgacgcgttt ggccgagcga
     4921 ttaccctgtt tcgccatatg tcgcagacga tagcggttga gggtcatcat tccggtttcg
     4981 gacccggaaa aataggctga aatgaccacc atgatgatca gaataatgat caacgtagta
     5041 gtagaaatgt gttccagggg aaactccttt tctgggttta gctgattaac tgctggacaa
     5101 ttcggctgcc gaagtaggcc agtgtcagaa ttaccgcgcc cgcaacgtta aaccagacga
     5161 cgcggcgtcc acgccatcct tcatggtaat gtccccacag cagcacaata tagacaaacc
     5221 acgccacgat agagagcaca gccttgtcga tattttccat gctaaatagg ttgtgcatgt
     5281 agaacaggcc agtgcaaagc gtgagcgtta gcagcaccac gccaatctgc gtgatgtgga
     5341 acattttacg ctcgatactc atcaatggcg gcatttcctg gttaaatgcc agcttcttgt
     5401 tcttcagttg gtaatcaatc cacgccagtt gcagcgcgta cagggcggcg ataattagcg
     5461 tggcatagga aaagagcgat aagccaatgt gcaccagcat cccaggcgtt gcttccagat
     5521 gggtgatgta ttcattgggc atgaaggtcg ccagcgccag gttgataagc gcaaaggcat
     5581 agacaatggg tagcagcaac cagccacgat tgcgagaagc cacaatggtc attaccgtac
     5641 agatcatcaa actgaccaat gaaccaacgt tcagcaggct gaggttttgt ccgctatcac
     5701 cgtcgggcag gatgcgggct tccagagcga ttgcgtggca gaccagcgca atgaccgcag
     5761 aaataatagc catgcgccgc cagccgccgt ttttttgcag cagaccggga acaatcagcg
     5821 caagactgac ggagtaggcg acaagcgcga gcagagcaaa aacgggcata gtgatgtcga
     5881 cagtttgagc aataagaaag agaagcagta tagcgtcagg tgaacgctgc tccaaccgtt
     5941 gcataacaac aaagacgcct tcatgttata ctgcggcaaa atactgatga tgtgtcgcga
     6001 ttgcggccaa ccgtttccac cccaggcgag agacaatgtt tgataattta accgatcgtt
     6061 tgtcgcgcac gctgcgcaat atcagtggcc gtggacgcct cactgaagac aacgtaaaag
     6121 atacgctgcg cgaagtgcgc atggcgctgc tggaggcgga tgtcgctctg ccggtagtgc
     6181 gtgagtttat caatcgcgta aaagagaaag cggttggtca tgaagttaat aagagcctga
     6241 cgccggggca ggagttcgtc aaaatcgtcc gtaacgaact ggttgcggcg atgggcgaag
     6301 agaaccagac cctgaacctg gcggcgcaac cgcctgcggt cgtgctgatg gcgggcctgc
     6361 aaggtgccgg taaaacaacc agcgttggta aactcggtaa gttcctgcgc gagaagcaca
     6421 agaagaaagt gctggtggtt tctgccgacg tttatcgccc ggcggcaatc aaacagcttg
     6481 agacgctggc agagcaggtg ggcgttgatt tcttcccttc tgatgttggt cagaagccgg
     6541 tagatatcgt taacgcggcg ctgaaagaag ccaaactgaa attctacgac gtgctgctgg
     6601 tggataccgc tggtcgtctg cacgttgacg aagcgatgat ggacgagatc aaacaagtcc
     6661 atgcgtcgat taacccggtt gaaaccctgt ttgtggttga cgccatgacc ggtcaggatg
     6721 cggccaatac ggcaaaagca ttcaatgaag cgttaccgct taccggcgta gtgttgacca
     6781 aagtggacgg cgatgcccgc ggcggtgcgg cgctctctat tcgtcacatc actggcaaac
     6841 cgatcaagtt tctcggtgtt ggcgagaaaa ctgaggcgct ggagccgttc catccggacc
     6901 gcatcgcgtc gcgtattctc ggcatgggcg acgtactgtc gctgatcgaa gatatcgaaa
     6961 gcaaagttga ccgcgcgcag gcagagaaat tagccagcaa gctgaaaaaa ggcgacggct
     7021 tcgatctcaa cgattttctt gagcagctgc gccagatgaa aaatatgggc ggcatggcta
     7081 gcctgatggg caagctgccg ggcatggggc agatcccgga taatgttaag tcgcagatgg
     7141 acgataaagt gctggtgcgt atggaagcca tcatcaactc gatgacgatg aaagagcgcg
     7201 ctaagccaga aatcatcaaa ggttcgcgta agcgtcgtat cgctgccggt tgcggtatgc
     7261 aggtgcagga cgttaaccgc cttctgaagc agttcgacga catgcagcgc atgatgaaga
     7321 aaatgaagaa gggcggaatg gcgaagatga tgagaagcat gaagggtatg atgcccccag
     7381 gcttccctgg tcgctaatcg gcctggcttg tttgccattt tggcaccggg atgtgctcgt
     7441 catcctcacg tactgcgtgt acgctccggt gactgcgcgc actccggcac caaactggct
     7501 gcaccgccga cggcctttta cagcagagtt agcaactgtt gattgcaatt cccgcaaaaa
     7561 tgagtaaaat tttcgggctt ttaatatgac accggactcc gttcctcgat ggggtccggt
     7621 tgttttattc acacaagagg atgttatggt aactattcgt ttagcacgtc acggcgctaa
     7681 aaagcgtccg ttctaccagg ttgttgtcgc tgacagccgt aatgcacgca acggtcgctt
     7741 catcgagcgc gttggtttct tcaacccaat cgctagcgaa aaagaagaag gcactcgcct
     7801 ggatctggat cgcatcgctc actgggttgg ccagggcgca actatttctg atcgcgttgc
     7861 tgcgctgatc aaagaagtaa acaaagcagc ttaatctgtc acggtggtca tgatgagcaa
     7921 acaactcacc gcgcaagcac ctgttgatcc catcgttttg ggaaaaatgg gttcgtctta
     7981 cggtattcgt gggtggctca gagtgttttc ttccaccgaa gacgccgaaa gcatttttga
     8041 ctatcagccc tggtttatcc agaaggcggg tcagtggcag caagtccagc tggaaagctg
     8101 gaagcaccac aatcaggaca tgatcatcaa gctgaaaggc gttgacgatc gtgatgcggc
     8161 gaacctgctg acgaattgtg aaattgtcgt ggattcatcg cagctgcctc agcttgaaga
     8221 gggggactac tactggaaag acctgatggg ctgccaggta gtaaccactg aaggctacga
     8281 tctcggtaaa gtcgtcgata tgatggaaac cggatctaat gacgttctcg tcattaaggc
     8341 aaacctgaaa gatgcgtttg gtatcaagga acgtctcgta ccgttcctcg atgggcaggt
     8401 tatcaagaaa gtcgatctca ctactcgttc aatcgaagta gattgggatc ctggttttta
     8461 aaccaccgga taaacggtaa aagacggcgc tatgtggatt ggcataatta gcctgtttcc
     8521 tgaaatgttc cgcgcaatta ccgattacgg ggtaactggc cgggcagtta aaaatggcct
     8581 gctgagcatc cagagttgga gtcctcgcga cttcacgcat gaccggcacc gtaccgtgga
     8641 cgatcgtcct tacggtggcg gaccggggat gttaatgatg gtgcaaccct tgcgggacgc
     8701 cattcatgca gcaaaagccg cggcgggtga aggcgcaaag gtgatttatc tgtcaccaca
     8761 gggacgcaag cttgatcaag cgggcgtcag cgaactggca acgaatcaaa aattgattct
     8821 ggtgtgcggt cgctacgaag gtatagatga gcgcgtgatc caaaccgaaa ttgacgaaga
     8881 atggtcaatc ggcgattacg ttctcagtgg tggtgagtta ccagcaatga cgctgattga
     8941 ctccgtttcc cggtttattc cgggagtact gggacatgaa gcctcggcaa cggaagattc
     9001 ctttgctgaa ggattgctgg attgcccgca ctatacgcgg cctgaggtgt tagaagggat
     9061 ggaagttccg ccagtgttac tgtcgggcaa ccatgccgag atacgtcgct ggcgtttgaa
     9121 acagtcgctg ggccgtacct ggcttagaag acctgaactt ctggaaaacc tggctctgac
     9181 tgaagagcaa gcaaggttgc tggcggagtt caaaacggaa cacgcacaac agcaacataa
     9241 acatgatggg atggcgtaag cccccgagat atcagtttac ccaggataag agattaaatt
     9301 atgagcaata ttattaagca acttgaacaa gagcagatga agcaggacgt accttccttc
     9361 cgtccgggtg ataccgtgga agtgaaagta tgggttgttg aaggttccaa aaaacgtctg
     9421 caggcattcg agggcgtggt tatcgctatt cgtaaccgcg gtctgcactc tgcattcact
     9481 gttcgtaaaa tttccaacgg cgaaggcgtt gagcgtgtct tccagactca ctctccggta
     9541 gttgacagca tttctgtcaa acgtcgtggt gctgttcgta aagctaaact gtactacctg
     9601 cgtgagcgta ctggtaaggc tgctcgtatc aaagagcgtc ttaactaaga tttcgcttaa
     9661 gcgacatcct gttaagaagg gctggccaat tggctggccc tttttttgtc tgtttgctcg
     9721 tgttttaagg ggttgtaatc accactgcga cgcggcggtt ctcggcgcgg ccctgggcgg
     9781 tcttgttact ggctatggga tatttttttc ctaaaccctg tgtggtgaga ttgctgcgtg
     9841 gaatttgtcc accaatagcc catgcatcgg ccacgacatt cgcccgtttc aatgataagc
     9901 cttcgttgta actgtcttca ccatagttat cggtgtgtcc atccatacgg gcatgtgtta
     9961 gccctgtcga ggccaatttg gctgccatgg tttggatctg ttgctggctt tccgggagca
    10021 atttgtagtc attttttgcg aacagaatgg catctgataa gcccagcgac cagtcgccgg
    10081 cggattcagt aaatccataa gattgcatag cggcgacttg ctcaggagta aactttccct
    10141 gaggggactg gcagccagtc agtattagtg aggtgaaaat caggggtgct actaggtgct
    10201 ttatcatatc gatatatcct tgttatctca ccagcttttc ggcacgctgg tgtttggcct
    10261 gatacatatt gtgatcggca agctcttgta atttttccgc agaggcgtgc tcaatggtca
    10321 tcgcgtaacc aatgcttaat gtcatggtgg tctgatggcc attatgaaga tcaaacggga
    10381 gattaaagat ttgtgtcagt gctgagcata tctgctgcac ttcagattct gactgtacat
    10441 catagagcac catagcgaat tcatcgccgc ccaggcggta tgctttatgt cgcagcccgc
    10501 caacttcagc taaccgtttt gcgatttcaa tcaagactct atcgcccgtc gcatgacccc
    10561 aggtatcatt gatgtatttg aaattatcgc catcaagaaa tagtaacgcc gacgtttttc
    10621 gggcatcgga attgttcatc aacgtgttga tgccgctacg aaacgctgcg cggttagcca
    10681 gcccggttaa tgggtcatgt agcgcggtac gtagaagctg cgcattttta gcctgtaaac
    10741 gaagctgcca ctcttccatt tcatccagca gactgttgaa gtcgagagcg aagcggtgaa
    10801 actcagcgat acgttcttcc gaaactcgtc gggaaaaatt gcggttggaa cgcacatcat
    10861 gtacgacatc ggtgatattt ttcagtgctt ccaccaggcc attgtgcaaa tggcgggtga
    10921 gggtaattgc gatgcctgat gccagcagaa tacaaccggt cagtacggcg agcgaaaacc
    10981 agataaaatg gctgattgaa ctgtcgcgag cggttaagcg tacttcgcca atggtttcac
    11041 cattgtgacg aatcggctga atgatggggg cggggaagag ccagtggctt atgaaattgc
    11101 tgaaagtatc gcctggatcc ttacgggtgt aatgccagga cgccagaata ttttgctgct
    11161 tatcacgtac ttctgcagtt gaaaattgcc cttgctggcc cagcgctgcc agtgtttcag
    11221 ttgctgcagg gccatcggca aaaacgacag ctgcttccag actgtaagt
//
LOCUS       DAJVQG010000127        11244 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJVQG010000127 DAJVQG010000000
VERSION     DAJVQG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11244
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.127"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..309
                     /locus_tag="OQM83_003090"
     CDS             <1..309
                     /locus_tag="OQM83_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCS1521291.1"
                     /translation="VVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQF
                     FDQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN
                     "
     gene            508..789
                     /gene="ppiC"
                     /locus_tag="OQM83_003091"
     CDS             508..789
                     /gene="ppiC"
                     /locus_tag="OQM83_003091"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCS1521292.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="OQM83_003092"
     CDS             complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="OQM83_003092"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCS1521293.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2501..3394
                     /gene="ilvY"
                     /locus_tag="OQM83_003093"
     CDS             2501..3394
                     /gene="ilvY"
                     /locus_tag="OQM83_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCS1521294.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="OQM83_003094"
     CDS             complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="OQM83_003094"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCS1521295.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="OQM83_003095"
     CDS             complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="OQM83_003095"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCS1521296.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="OQM83_003096"
     CDS             complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="OQM83_003096"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCS1521297.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="OQM83_003097"
     CDS             complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="OQM83_003097"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCS1521298.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8117..9763)
                     /locus_tag="OQM83_003098"
     CDS             complement(8117..9763)
                     /locus_tag="OQM83_003098"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCS1521299.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9766..9816)
                     /locus_tag="OQM83_003099"
     CDS             complement(9766..9816)
                     /locus_tag="OQM83_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521300.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="OQM83_003100"
     CDS             complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="OQM83_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCS1521301.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10354..11244
                     /locus_tag="OQM83_003101"
                     /pseudo
     CDS             10354..11244
                     /locus_tag="OQM83_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
BASE COUNT         2554 a         3090 c         2951 g         2649 t
ORIGIN      
        1 gtcgtgctca acggaaaaat taatggcaac gcatgtatcg tcgttccatt atcaaccact
       61 cgcgatcatg acaagttaaa tcgtggaatg catgttgaaa tagccagtaa tgttatcaat
      121 gacctgcaat tctttgatca gcagattcgt tgggccaaag ccgacctagt acaacaagtc
      181 agtcgaaacc gattaaacag agcaaggact tacagagggt atcttaatca atgtttgcca
      241 catgagttgg ttgcagacat acagagggcg gtcatcaagt caatcaatgc catatcgctc
      301 ataaattgac aataaaataa ccatccccga taatgacttt gtagcccata gaggccctaa
      361 gttttctccc cttgagggac ctgcgaagcc cggattttcc gggcttttgc catatatgta
      421 ccagcccctc cctgactaac aattacgtta gcatctgcgg atcatgctaa aatcgccgcc
      481 tgacaattat tcactcaagg cacgatcatg gcaaaaacag cagcagcact gcatatcctt
      541 gtaaaagaag agaaactggc tctggatctt ctcgagcaga ttaagaacgg tgccgatttc
      601 ggcaagctgg cgaagaaaca ctccatttgc ccatcaggca aacgcggcgg tgatttaggt
      661 gaattccgcc agggtcagat ggttccggcg tttgataaag ttgtgttctc ttgtccggta
      721 ctggagccaa ccggcccgct gcacacccag ttcggttacc acatcattaa ggtgctgtac
      781 cgtaactaat agcaaggcct tctccaggag aaggccttga gtgttttctc cctctccctg
      841 tgggagaggg tcggggtgag ggcatcagcg cgcacttaac ccgcaactgc aatacgtttc
      901 atatccgtca tatagccgcg cagtttctta cctacctgct caatcgcatg gcagcgaatc
      961 gcttcgttca catcacgcag ttgcgcgtta tctaccgcac cttccggaat agctttaccc
     1021 agatcgcccg gttgcagctc tgccataaac ggtttcagca gcggcacaca agcgtaagag
     1081 aacagatagt taccgtactc ggcggtatca gagataacca cgttcatttc gtacagacgc
     1141 ttacgggcga tggtgttggc aatcagcggc agttcgtgca gtgattcata gtaagcagat
     1201 tcttcgatga tgccggaatc aaccatggtt tcaaacgcca gctcaacgcc cgctttcacc
     1261 atcgcgatca tcagtacgcc tttatcgaag tactcctgct cgccgatttt gccttcatac
     1321 tgcggcgcgg tttcgaaggc ggttttgccg gtctcttcac gccaggtcag cagtttctta
     1381 tcatcgttgg cccagtccgc catcataccg gaggagaatt cgccggagat gatgtcgtcc
     1441 atatgtttct ggaacagggg tgccatgatc tctttcagct gttcagacag cgcgtaagca
     1501 cgcagtttcg ccgggttaga gagacggtcc atcatcaggg tgatgccgcc ctgcttcagc
     1561 gcttcggtga tggtttccca accgaactga attagttttt ctgcgtatgc cggatcggtg
     1621 ccctcttcca ccagcttgtc gaagcacagc agagagccag cctgcaacat accgcacagg
     1681 atggtttgct cacccatcag gtcagatttc acttccgcaa cgaaggacga ttccagcacg
     1741 cccgcacggt gaccaccggt tgctgccgcc catgctttag cgatcgccat gccttcgcct
     1801 ttcggatcgt tttccgggtg aacggcaatc agcgtcggta cgccgaatcc acgtttgtac
     1861 tcttcacgta cttcggtgcc agggcatttc ggcgcaacca tgactacggt aatgtcttta
     1921 cggatctgct cgcccacttc gacgatgttg aaaccgtgcg agtaacccag cgccgcgccg
     1981 tctttcatca gtggctgtac ggtgcgcact acatcagagt gctgcttgtc tggcgtcagg
     2041 ttaaccacca gatccgcctg cgggatcagt tcttcgtaag taccaacttt aaaaccgttt
     2101 tcggtcgctt tacgccagga cgcgcgcttc tcggcaatcg cttctttacg cagagcgtag
     2161 gagatatcga gaccagaatc acgcatgttc aggccctggt tcagcccctg tgcgccacag
     2221 ccgacgataa ccactttttt accctgaagg tagctcgcgc cgtcggcgaa ttcgtcgcgc
     2281 cccataaagc gacatttgcc cagctgtgcc agctgctggc gcaggttcag tgtattgaag
     2341 tagttagcca tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt cgcgtttgcg
     2401 agattgaatt cactatatga caggaaattt attgcggaaa ttgatatatt cacaacgtca
     2461 cattgcaatt tctgcaacgt caacatcgag ggctgcgcca ttggatttac gcgatctgaa
     2521 aaccttcctg catctggcgg aaagccgcca ttttggccgc agcgcgcggg cgatgcacgt
     2581 tagcccatcc acgctctcac ggcagattca gcgcctggaa gaagatcttg gtcagccgct
     2641 atttgtgcgc gataaccgca cggtaacgct gacggaagcg ggcgaagagc tgcgcgtttt
     2701 cgcccagcaa acgctgttgc agtatcagca attgcgccac accatcgatc agcaagggcc
     2761 gtcgctctct ggcgaattac atatcttctg ctcggtgacc gctgcctaca gccatctgcc
     2821 gccgattctg gatcgcttcc gcgcggaaca cccgtcggtg gagatcaaac tcaccactgg
     2881 tgatgcggca gatgcaatgg aaaaagtggt cacgggagaa gcggatctgg cgattgccgg
     2941 gaaaccggag acgctacccg gcgctgtggc gttttcgatg ctggagaatc tggcagtagt
     3001 gctgattgcc cccgcgctgc cctgcccggt gcgtaatcag gtatctgtcg aaaagcccga
     3061 ctggtcaacg gtgccgttta ttatggccga tcaggggccg gtacgccgcc gcattgaact
     3121 gtggtttcga cgcaataaaa tcagcaaccc gatgatttac gccacggttg gcgggcatga
     3181 agcgatggta tcgatggtgg cgctcggctg tggtgtggcg ctgttgccgg aagtggtgct
     3241 ggaaaacagc ccggagcctg tacgcaaccg ggtaatgatt ttagatcgca gcgatgagaa
     3301 aacgccgttt gaactgggcg tgtgtgcaca aaaaaagcgg ctgcatgagc cgctgattga
     3361 ggcgttctgg aggattttgc cgaaccacaa atgacgttgt cgcgcgggta ggcctgataa
     3421 gcgaagcgct atcaggcatt tttccctaac ccgccaaaaa gaacctgaac gccggattat
     3481 tggtttcgtc gtggcaatcg tagcccagct catttagccg ggtttcgaaa tccggttcat
     3541 ggtcgccgag ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt
     3601 ggaacaaaga aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg
     3661 gtgattccgg gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc
     3721 cgaccatata gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt
     3781 agccgccgtc gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc
     3841 gcacgccgac aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga
     3901 ccgatcgccc gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg
     3961 tcaccgccaa caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca
     4021 ggccgtggaa gttcacgttg gcaccggaaa gaatatgcgc caggcgttcg ccgcgaatgt
     4081 tgtgctgagc gatatatttt ttcattcccg ccagcgccaa cgcgccagag ggttccgcca
     4141 ctgcgcgcac atcttcgaat aaatccttca tcgccgcaca gatcgcatca ctatcgacgg
     4201 tgatgatgtc gtcgagatac tcctggcata aacggaaggt ttcgtcgccg atgcgtttta
     4261 ccgcgacacc ttcggcgaac agtcccacgc gcggcagatc aaccggatga cccgcatcca
     4321 gcgctgcttt caggcaggcg gagtcttccg cttctacggc gatcactttg atttgtggca
     4381 tcagttgttt gatcagcacc gcaacgcccg ccgccagacc gccgccgccg actggcacaa
     4441 atacgcggtc gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg
     4501 caatcaccat cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt
     4561 cgatcgcttt agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga
     4621 atccacgtac cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct
     4681 tcacgcctaa ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag
     4741 tgatcacgcc gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc
     4801 gcagcttaaa gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa
     4861 gacgcgacga cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg
     4921 gcgcgcgcag cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg
     4981 agtcagccat tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct
     5041 ggttgccagg ctggcgtagg cgcgcagggc aaaggaaacc tgacgttcac ggtttttcgg
     5101 cgtccaggct ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc
     5161 gcttacctgt aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc
     5221 aatcagacca atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc
     5281 agaggtgccg ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat
     5341 tgatttcagg aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc
     5401 ttcatagcgg atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac
     5461 cgcgtcgtcc tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc
     5521 gacgcccgct gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag
     5581 gccgccgtct ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc
     5641 atcgagagaa tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg
     5701 gcctgcgcgg aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc
     5761 cagagtttgc ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc
     5821 gcgatccagt tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg
     5881 gtatttttgg gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc
     5941 gatatcactc atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac
     6001 cgtgttggta gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt
     6061 gctagcaata ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa
     6121 ttcaacaatg cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag
     6181 cagcgagccg ttgcctggct gcgacaaacc cagcgcttcg gtcaggcagt tcattgagtt
     6241 agcggtaaac atcccggagc aggaaccgca ggttgggcag gcggaacgtt caacctgatc
     6301 gctctgggag tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag
     6361 cttgatgatc tgctcggaca gtttggtttt cccggcttcc atcgggccgc cggaaacaaa
     6421 gatcaccgga atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca
     6481 gttagagatg cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc
     6541 agcgatcagt tcgcgagatg gcagtgaata aagcatcccc ccgtggccca tagcaatccc
     6601 atcatccacc gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc
     6661 ggcgaccagt ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga
     6721 gttcacaacc gcgataatcg gcttgccaaa atcggcatcg gtcattccgg tggcgcgcca
     6781 cagcgcacga gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg
     6841 catactttat ttactcccag tgtctgtctc gtaaatggga cggtaagtgc cgtcccattt
     6901 tttgtattta ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga
     6961 agaggccgaa gaaggcttgc tgaatgcatt tggtaaccgg gccgcaacgg ccttcaccca
     7021 cctgaatacc gtctacactg cgcactggcg tgatttctgc cgccgtaccg gacataaaca
     7081 cttcatccgc caggtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca
     7141 gctctttcgc cagtttgatg atggcatcac gggtaatacc cggcagcgcg gatgaggtaa
     7201 acggtggggt gaacagcacg ccgtctttca cttcaaacag gttttcacct gcgccttcag
     7261 agatgtaacc gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc
     7321 tacccaccag cagggaagag aggtagttac caccggcttt tgccgccgtc gggatggtgt
     7381 ttggtgctgc gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg
     7441 cgcccagata cgctccccac gggaaagcag cgataatcac gtcggttgag tatcccgctg
     7501 gcgggtttac tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg
     7561 tgagattatt tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgcg
     7621 aaaccgggaa gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc
     7681 ggaatacaac cggtcctttg tgcgagtcgt agcaacggat gccttcaaaa accgaagtgc
     7741 catagtgcag cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc
     7801 cattgaacca aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag
     7861 gcgcggattt gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc
     7921 agtttattta actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg
     7981 atatttatat tttgtgcatc gctggcggcg gccatattca ttgagcagac gtggaaacca
     8041 cgatgacgca ccacgcgtaa aacacgttct aaggtttctg gattgaagcg agccgataca
     8101 ttgacctgat gttgcatcat gataatttct ccaacatttc tgaattactg gcgccaggcg
     8161 gcaccagcgg ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat
     8221 cactgttcag catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc
     8281 cagggatgcc gaaggcgctg gctaacatga ggaaatcggg gttatcagta agggtggttt
     8341 cgctgtatcg ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt
     8401 tatcgagtaa gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca
     8461 cattcatcat gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga
     8521 cttgtgcgcc aaccgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga
     8581 tgaaattttc cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca
     8641 catctgtggt cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg
     8701 gcgcgtagat agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg
     8761 cgcagtattg ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat
     8821 ttaaatcacc ctgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga
     8881 tatccatatg gataacactg gcgtgtggtg cgaaagtatt cagtttgccg gtcacccggt
     8941 catcaaaacg tgcgcccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg
     9001 ctttgatgcc gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc
     9061 ccagcccttt cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag
     9121 ccggaactgc ctgcgccata cccacgccac cgccaacgta cagcatcggt ttttgcgctt
     9181 ttgccagcat ctggcgcgct tgctcaactt cggcatgtgg gaaagtcact tcgttttcaa
     9241 cggtggtgaa ccacggttcc aggtcaccgc tggctaactg gatatctttt gggatatcga
     9301 ccagaaccgg accaggacga cctgagcagg caacgtcgaa tgcttcagcc atgatgcgcg
     9361 gcaactcttc cagcgactgc accagaaagc tgtgcttggt acaggctaac gacaatccta
     9421 ggacatccac ttcctgaaat gcgtcagtgc cgataaacgg tgcggacact tgaccggtga
     9481 tggcaacaac agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg
     9541 cgcccggacc agacgtggcg atacatacgc cagttttgcc ggtagcacgg gcataaccga
     9601 tagccgccat tgccgcaccc tgctcatgtc ggcatagcaa gtgctccacg ccgccgtcat
     9661 acaatgcatc gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac
     9721 cctgtgcccg caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct
     9781 gaatcttgag aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt
     9841 aaggtcaaaa aaaacccccg gacctttcgg tgcgggggtc ttagttcgtt aaggcttgat
     9901 ctctaagcct ttcctcgtcc aagtgcagcc ccgcacggtg ggataataat caccaccacg
     9961 ctaatcacga ctaggctaat cactcgtaga agggctgtca ttttgtcttt tcttgcatct
    10021 tgttcgaagg aatgcctaaa gagttaccat agattttgcg aatagcacaa gatatttttg
    10081 ggcctatttt tatgatagct ttaataattt caatagaagt tgttgttttt taaaaagaaa
    10141 atagtgagtg aaaaaaattc aatttcagta taaatgttgt tcccttcctc gtagttcatc
    10201 ctttgtccgc tgcatttttg cgagcatcat tccaccgcca gaacaactca acttcacttg
    10261 cacaaataaa caggaatcag ccactaaaat tcacgaatta gcgggctaca gcagcgatat
    10321 cttatgcaca taatggtgac tcaaggaggg cttatgtcac tgtcaattgt tcatacccgc
    10381 gcagccctgg gagtaaatgc gccccctgat cactgttgag gtacatatca gtaaaggtct
    10441 acccggctta acgatggtgg gcttaccaga aacaactgta aaagaagctc gcgatcgcgt
    10501 gcgcagcgcc attatcaata gcggatatga atatccggcg aaaaaaatca ccatcaacct
    10561 tgctccagcc gatctgccaa aagaaggggg acgatatgat ttacctatcg ccattgcgtt
    10621 gctggcggcc tcagaacagc ttacagccaa taagttagat gaatatgaat tagtcggaga
    10681 actggctctt acaggcgctc tgcgtggcgt tcccggtgca atctccagtg caactgaagc
    10741 tattaagtcg ggcagaaaaa ttatcgtcgc gaaagataac gaagatgaag tggggctaat
    10801 taacggtgaa ggatgcctga tagccgatca tctgcaagct gtctgtgcgt ttctggaagg
    10861 taagcacgct ctcgaacgcc cgaaaccaac tgatgcagta tcccgggcgc tacaacatga
    10921 tctcagtgat gttgtcggtc aggaacaagg aaagcgagga ctggaaatta ccgccgctgg
    10981 caggcacaac cttttactga ttgggccgcc gggaacaggt aaaacaatgc tcgccagccg
    11041 tattaatggc ctgttgccag atttaagcaa tgaagaggca ctggagagcg ctgcgatatt
    11101 aagtctggta aatgctgaat cagtacaaaa acaatggcgg cagcgcccgt tccgctcacc
    11161 tcatcacagt gcgtcgttaa ctgcgatggt gggcggtggt gcaattccag ggccaggtga
    11221 aatttcgttg gcgcataacg gcgt
//
LOCUS       DAJVQG010000128        11065 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJVQG010000128 DAJVQG010000000
VERSION     DAJVQG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11065
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.128"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..208
                     /locus_tag="OQM83_003102"
     CDS             <1..208
                     /locus_tag="OQM83_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1521302.1"
                     /translation="IVPLTVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFA
                     QTTKGVLETGIYRGVVSIYLSQDI"
     gene            300..2984
                     /locus_tag="OQM83_003103"
     CDS             300..2984
                     /locus_tag="OQM83_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCS1521303.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLAHHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            2981..4069
                     /locus_tag="OQM83_003104"
     CDS             2981..4069
                     /locus_tag="OQM83_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCS1521304.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(4152..5449)
                     /gene="exuT"
                     /locus_tag="OQM83_003105"
                     /pseudo
     CDS             complement(4152..5449)
                     /gene="exuT"
                     /locus_tag="OQM83_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            5932..7344
                     /gene="uxaC"
                     /locus_tag="OQM83_003106"
     CDS             5932..7344
                     /gene="uxaC"
                     /locus_tag="OQM83_003106"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCS1521305.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            7359..8846
                     /gene="uxaA"
                     /locus_tag="OQM83_003107"
     CDS             7359..8846
                     /gene="uxaA"
                     /locus_tag="OQM83_003107"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCS1521306.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINIRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8929..9480
                     /locus_tag="OQM83_003108"
     CDS             8929..9480
                     /locus_tag="OQM83_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCS1521307.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9485..10729)
                     /gene="sstT"
                     /locus_tag="OQM83_003109"
     CDS             complement(9485..10729)
                     /gene="sstT"
                     /locus_tag="OQM83_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCS1521308.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         2921 a         2690 c         2873 g         2581 t
ORIGIN      
        1 gattgttccg ctgacagtaa cctggggcgg tgaagaaatt aaagctgatg cggcaaccac
       61 ttttaccgca accaaaatct ttgcttccga tgcgttaact aacggttctc tggctaaaaa
      121 cctgatgttt gctcaaacca ccaaaggtgt tctggagaca ggcatctacc gtggtgtagt
      181 aagcatttat ctgtctcagg atatctaatc cagataacag aattaacacg taacaaaggg
      241 aggggtagcc ctcccttatt ttaactgttt tagtatttgt tatggatttc aggattgcca
      301 tggataaaaa attactggct cttttgatcc tggcgagtct cagcccggca gaggcgacat
      361 taaccgaaat tcccgcaggg tttgaggtta ttgctcaggg acagcaggag tatatcgagg
      421 tttatttttc agggaaaagt ctcggtaaat attatgcaat ggttaatctt gataccgtaa
      481 catttcttga tccagcaagt ctatataaca agctggaact ggatgtagac gatcagaaaa
      541 tcgcgcatat agtgaaagaa aaattatcgc agccgctagc tcaccacggt gaattggctt
      601 gcggttatgt acgtactgac tcagggtgtg gttttctgaa taccgatacg ctggaaataa
      661 tctataatga tgaagaaagt tcggcaacgt tgtttattaa tccgcaatgg aattcagctt
      721 tcgatgcgaa gtcattatat ttaaatccag acaaaaatac tgttaatgct tttatacatc
      781 agcaagacat caatgttctg gcacaggatg attaccaatc gttgtctatt cagggaaacg
      841 gtgcgctggg aataacagaa aatagttata ttggtgcaca ctggaatttc aacggttatg
      901 atgcagatga tgtcagtgac agtaatgctg acgtcagcga tctctattat cgttatgatt
      961 ttttacgtcg ttattatgtg caggcgggtc gcatggacaa ccgcacacta tttaatgcac
     1021 aaggcgggaa ctttaccttt aactttctgc cactcggtgc aattgatggg atgcgtatcg
     1081 ggtcgacact gagctattta aaccaggcgc aaagccagca aggaacccca gtaatggttc
     1141 tgctttcgcg caattctcgt gttgacgctt atcgtaatga gcaacttttg ggatcgtttt
     1201 atctcaatag tggttcgcaa tttattgata ccagttcctt tccgccaggc agctacagcg
     1261 tggcattaaa agtctacgaa aataaccaac tcacccgcac cgagcttgtg ccgtttacca
     1321 aaaccggcgg tctgactgac ggaaatgcgc aatggttctt acaggcaggt aaaactacat
     1381 cacaggtttc tgatgatgaa agttcagctt atcagctagg agtacgcctg ccattacatc
     1441 cgcaatatga gctctacgca gggctggcga atgccgatga tgtgagtgct ttcgagttag
     1501 gtaataactg gacggcagat ttaggcgggg tggggaatct tgcaatcagc gccagcgtgt
     1561 tccgtaacga tgacggcggc aaaggtgata tgcaacaggc caactggagt aatccgggat
     1621 ggccgacgtt gggcttttat cggaccaact ctgacggcga tgcttgtaca accgacagca
     1681 gagagagcta taacgcctta agctgttatg aaagtatttc cgcgacggtt tcacagaatt
     1741 ttgtcggctg gaatatgatg ctgggttata cccgcacaca aaataacact gatgatagtt
     1801 tgcgttggga taaacagcag agctttgaaa ataactatct tcgccagaca acagcgcaaa
     1861 gtatctctga aactgtacaa cttagcgctt cccgcgcttt tgtgatgcgt gactggattt
     1921 tgagtacgtc agtcggtgtt ttccatcgta atgacaacgg tggcgataac gacgacaacg
     1981 gcttgtactt atcgttttcg ttatctgaca cgccaacgat ggatagcaat aacaacagcc
     2041 attcaacaaa tgtgtctacg gattatcgtt atagcgaaca ggatggcgat caaacgtcat
     2101 ggcagttatc gcataccttt tataacgatt cattcagcca taaagaactt ggcgtaaccg
     2161 tcggaggcct gaacaccgat accataaaca gcgcggttaa cgggcgttgg gatgggcaat
     2221 acggaaatgt ctacgctacc gtatctgata gttatgaccg taagaatcat gatcatcttt
     2281 cggcctttac gggcacttat agctctacgc tggctgtcag ttgctatggc gttaatttgg
     2341 gagccagtgg tacagacgat ttgctgggtg cggtactggt ggatgtgaaa ggcttctctg
     2401 aacaggatga agagagtcag gatctgcaac tcgaagcgcg ggtggcaggc agccgaactt
     2461 tgcagcttgg tcaaagtgac agtgtgttgt tcccttatcc tggatttcag tctggttttg
     2521 ttgaggttaa cgacagtagc cagggcaatc agcaggggac aacaaacatc attaacggtg
     2581 cagggaatcg tgaattaatg ttgttgcctg gcaagctgcg ctatcgcgaa gtgtctgcca
     2641 gctttaacta caactatatc ggtcgtttat tactgccggc agcggtgaaa aaattcccga
     2701 ttgtggggct gaacagcgcc atgttactgg tagctgaaga tggaggattt acacttgaaa
     2761 ttaacggcag cgaaaaagag ctctatctgc tttccgggca gcaattcctt aagtgtccac
     2821 tgagtgttgt aaagaaacgc gccagcattc gttacagcgg agatgttact tgtagtgtgg
     2881 tgacttattc acaattaccg gagtcgattc aggttcaggc acagttgaaa cagcctaaat
     2941 tacgtggaaa cgttcagacg gcgcaaaggg aggttgcacc atgagaaatc gattgattgc
     3001 ggcgatattg ggcttgtttg gcacgctcac tggcgttcag gcagctcctg acgtgaccag
     3061 tgaaattacg tatgatttgg catctggtag agcggattat tacttctgga aggatgaagc
     3121 gtcggcagga aataatggat atatgtggta tgaatgttcg tatcctgacc tccaacaaac
     3181 ctgtacagct aatggaaata tatcgacagt acaaatctat ttaactgaac aacgcagtgg
     3241 gatgcgttgg ccggtaaaac tcaaaggatt taaaacagcc attgtaagta gcgatgaagc
     3301 gccgccagga tgcaaggggg gcaaagggct tcagacgaat cttaaggatt ctaatagatc
     3361 ttcatgtaca gaagatggtc aacattatta tatatacgat acaaagtttc ttacgctcta
     3421 ccttgagcag acagagatga agaatttgcc gattggtggc gtctggaagg ggaaagttaa
     3481 attacattcg aacagcccgg cccaggacta tttcgcaaat attaccctga atacgctcga
     3541 ccccaaccat attgacgtgt tcttcccgga gttcgcccac gccacgccaa gggtgcagtt
     3601 agacttgcat ccaacaggaa gcgttaacgg cagcaactac gcgcaagatc tgaccatgtt
     3661 ggacatgtgc ctgtacgatg gttttaacgg taatgccatc agttatgaaa tcatgctcaa
     3721 agatgaaggg cgaccagctg cagggcgcag agacggtgac ttctctatct atcgtcaagg
     3781 agggaccacc accgacgagg gagaacgcat tgattaccgg gtcaaaatgt acaacccgga
     3841 aaccggtggg caaattgatg tgcgcaataa tgaaaatatg gtctggaaca gcattaacct
     3901 gaaacgtgtg cgtccggtgg tactgcccgg tattcgctat gccgtgatgt gtgtgccgac
     3961 acccttgact ctggcagtcg ataaattcag cgtgatggat aaacaggccg ggtactacat
     4021 gggcaaattg tcagtaatct ttacgccttc cttgccaacc atcaattgac cccgcagacc
     4081 agaaaaaact aaacaacgaa gccgcctgat aaggcggctt cgtcattaca acttacgtta
     4141 aacgttaaac gttaatgttg cggtgcggga tcgttatgtg tttcctgcgc cacctcaatc
     4201 gccggtttgt tctgcaatac ggtccagata accagcgcac ccagcaggtc aaatactgcc
     4261 agaactgcga acagcgggct gaagccgatg gtgtcagcca gtgcaccgac aaccagcgca
     4321 aacagcgtac ttgccagcca tgcggacatc ccggttaaac cgttcgccgt cgccacttcg
     4381 ttacgaccga acacgtcaga agagagcgta atcagcgcgc cagacagtgc ctggtgggca
     4441 aaaccaccga tacacagcag cataattgcg acatacgggt tggtgaacag accgatcata
     4501 cccgggccaa tcatcagcac agcacccagc gttacgacca tcttacggga aacgatcagg
     4561 ttcacaccaa accaacgctg gaacagcggc ggcaggtaac caccgaggat acaaccgagg
     4621 tcagcaaaca gcatcggcat ccaggcgaac atcgcgatct ctttcaggtt aaagccgtaa
     4681 actttaaaca tgaacagcgg gatccacgcg ttaaaagtac cccaggccgg ttctgccagg
     4741 aaacgcggca gtgcgatacc ccagaactga cggttacgca ggatctgacc aacggacatt
     4801 ttcttcgccg tgctcacctg gtgctgggct tcctgaccat taataatata gtcgcgttct
     4861 tcatcggtca gatgcttctg gtcgcgcgga tgtttataga aaatcagcca tgccatcgcc
     4921 cagataaagc tcaatgcacc ggagatgata aatgccatct gccagctgtg catcacgatt
     4981 gccataccac cagcggcggc gcaatcatcg caccaatcga agaacctacg ttaaagtagc
     5041 ctactgcgat ggaacgctct ttcgccggga accattcgga gctggctttc agacccgccg
     5101 ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc aacagccagg ccaccccagc
     5161 tacctgccag cgcggttgca ccacagaaca cggcccacag cacagcaaac attgcataac
     5221 cgattttcgt acccagcaca tccagtacat aacctgctac cggttgcatg accgtataag
     5281 cagcagaata ggctgcgatg atataggaat actgttgggt ggagatgttt aactcttcca
     5341 tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag gtaaccaagc acggtgccga
     5401 gcgtcaccag tgcgatcata taccaacgta accctttaat tttacgcatg taaaacctca
     5461 tcgttttgtt atatccgcag gaagcgcggc catcatcccg ccagaggctg aagatgttag
     5521 ttacgaccgg gtaaccgaaa aaaaaccggc acgccccgaa cgttgccatg aaagtttcgt
     5581 gctaattcgg cttccgtacc ggttaacccg attcatctaa caaatgaatg aatcggcaat
     5641 ttgcattccg tcgacttatg atttgcgacg gcagaaagat aacttgtcat acaactttaa
     5701 aaggtgagag ccatcacaaa tgtgcgaata tttgtaggga cattacctga cgacagcgag
     5761 gccagtactg gcgcggcctg cagcgagatt tatcactttg agagtaattt ttttaactac
     5821 gtttattgat ctaactcacg aaaatatctt cggactctgg aaattggtgt gataactttg
     5881 tcagcatcgc accataagca agctagctca ctcgttgaga ggaagacgaa aatgactccg
     5941 tttatgactg aagatttcct gttagatacc gaatttgccc gccgtctgta tcacgactac
     6001 gcaaaagacc agccgatttt cgattaccat tgccatttgc cgccgcagca gattgcggaa
     6061 gactatcgtt ttaaaaacct gtatgacatc tggctgaaag gcgatcacta caaatggcgc
     6121 gctatgcgta ccaacggtgt ggccgagcgt ctgtgtaccg gtgatgcatc tgaccgtgaa
     6181 aaatttgacg cctgggcggc gactgttccg cacactatcg gcaacccgtt ataccactgg
     6241 acgcaccttg aactgcgtcg tccgtttggt atcactggca aattgctttc tccgtcaact
     6301 gccgatgaaa tctggaacga atgtaacgaa ctgctggcgc aggataactt ctccgcgcgc
     6361 ggcatcatgc agcagatgaa cgtgaaaatg gtcggcacca ccgatgaccc gatcgattct
     6421 ctggagcatc acgcagagat cgccaaagac ggctctttca ccatcaaagt gctgccgagc
     6481 tggcgtccgg acaaagcctt caacatcgaa caggcgacct ttaacgacta catggcgaag
     6541 ctgggcgaag tttccgatac cgacattcgc cgctttgctg acctgcaaac tgccttgacc
     6601 aaacgtctgg atcacttcgc cgctcacggc tgtaaagtgt ctgaccatgc gctggacgtg
     6661 gtgatgtttg ctgaagcgaa cgaagcggaa ctggacagca ttctggcgcg ccgtctggct
     6721 ggcgaaaccc tgagcgagca cgaagtggca cagttcaaaa ctgcggtact ggtgttcctt
     6781 ggtgccgaat atgcacgtcg cggctgggta cagcagtacc acattggcgc gctgcgtaat
     6841 aacaacctgc gtcagttcaa actgctgggg ccggatgtag gctttgactc catcaacgac
     6901 cgtccgatgg cggaagagtt gtctaagctg ctgagcaagc agaacgaaga aaacctgctg
     6961 ccgaaaacca ttctctactg cctgaacccg cgcgataacg aagtgctggg caccatgatc
     7021 ggtaacttcc agggcgaagg tatgccgggc aaaatgcagt tcggttccgg ctggtggttt
     7081 aacgatcaga aagacggtat ggaacgtcag atgacccaac tggcgcagct cggtctgctg
     7141 agccgctttg tcggtatgct gactgacagc cgtagcttcc tgtcatacac ccgtcacgaa
     7201 tacttccgcc gcattctgtg ccagatgatc ggtcgctggg tggaagcggg cgaagcaccg
     7261 gcggacatca acctgctggg cgagatggtg aaaaatattt gctttaacaa tgcgcgtgac
     7321 tacttcgcca ttgaactgaa ctaaggtctg gggttgatat gcaatacatc aagatccatg
     7381 cgctggataa cgtcgcggtc gctttagcag atttggctga aggcacagaa gtcagtgtcg
     7441 ataaccagac tgttacgctg cgccaggatg ttgctcgtgg acataaattt gcgttaacgg
     7501 atatcgcaaa aggggccaac gtcatcaaat atggcctgcc gattggttat gcattggcgg
     7561 atattgcggc gggggaacac gtccacgccc acaatacgcg cacgaatctg agcgatctgg
     7621 atcagtatcg ctatcaacct gattttcagg atctgcctgc gcaagcggca gatcgtgaag
     7681 tgcagatcta tcgtcgcgct aacggcgatg tcggggtgcg taacgagctg tggatcctgc
     7741 caaccgtggg ctgtgtcaac ggcatcgcgc ggcaaatcca gaaccgtttc ctgaaagaga
     7801 ccaacaacgc cgaaggtacc gacggcgtgt tcctcttcag ccacacctac ggctgctcac
     7861 aactgggcga cgatcacatc aacatccgca ccatgctgca aaacatggtg cgccacccga
     7921 acgcgggcgc agtgctggtg attggtctgg gctgtgaaaa caaccaggtt gccgcattcc
     7981 gtgaaacgtt gggcgatatc gatcctgaac gcgttcattt catgatctgc caacagcagg
     8041 atgatgagat cgaagccggg atcgagcatt tgcatcagct gtataacgtg atgcgcaacg
     8101 ataaacgcga gccaggcaaa ctcagcgaac tgaagtttgg tctggagtgc ggtggttctg
     8161 acggtctttc tggtattact gctaacccga tgctggggcg tttctctgac tacgtgattg
     8221 ctaacggcgg tactaccgta ctgaccgaag tgccggagat gtttggcgca gagcagttgc
     8281 tgatggacca ttgccgcgac gaagcaacgt ttgaaaaact ggtcaccatg gtcaacgact
     8341 ttaaacagta ctttattgcc catgatcagc cgatctacga aaacccatca ccggggaaca
     8401 aagcgggcgg tatcaccacg ctggaagaca aatcacttgg ctgtacccag aaagcgggtt
     8461 ccagtgtcgt ggttgacgtg ctgcgttacg gcgagcgtct gaaaacgcct gggctgaact
     8521 tgttaagtgc gccgggtaac gatgccgtag cgaccagcgc cctggcgggt gcgggctgcc
     8581 atatggtgct gttcagtact ggtcgcggca cgccatacgg tggatttgtg ccgacggtga
     8641 aaatcgccac caacagtgaa ctggcggcga agaaaaaaca ctggatcgac tttgacgcgg
     8701 gtcagctgat ccacggtaaa gcgatgccgc agttgctgga agaatttatc gacaccatcg
     8761 ttgagtttgc caacggtaag caaacttgca acgagcgtaa cgacttccgc gaactggcga
     8821 tcttcaaaag cggcgtaacg ctataaagtc gaaatgagtt gttaataaaa aacggcgttt
     8881 cataatgtga agcgccgttt gcttttccat acctgataag gatttatcat gaccgcgtat
     8941 tggctggccc agggcgtggg tgtcatcgcc tttctgattg gtatcacaac atttttcaat
     9001 cgtgacgaac gtcgcttcaa aaagcagctt tcggtctata gcgccgttat tggcgtacat
     9061 ttttttcttc tgggcaccta tcccgctggt gccagtgcca tccttaatgc cattcgtaca
     9121 ttgattacct tacgcacgcg cagcttatgg gtaatggcga tttttattgt gctgactggc
     9181 ggaattggcc tcgcgaagtt ccatcatcct gtcgaactat tgccggttat cggtacgatt
     9241 gtcagtacct gggcgctgtt ccgctgtaaa gggctcacca tgcgctgcgt gatgtggttt
     9301 tcaacgtgtt gctgggtgat tcacaacttc tgggcggggt cgataggcgg cacaatgatt
     9361 gagggtagtt ttctgcttat gaatggcttg aatatcattc gtttctggcg gatgcagaaa
     9421 aggggaattg atccgtttaa agtagagaaa acccctcccg ccgtagacga aaggggttaa
     9481 acaattaatt acgcagggcg ctatttgcca gacgatcgtc ttccgcctgg caagctgccg
     9541 cagtgaacag cacgtcagtt gaagagttca gcgcggtttc gcaagagtcc tgcaatacgc
     9601 cgatgataaa gccgacggca accacctgca tggcgatatc gttcgaaata ccgaacatat
     9661 tacaggccag cgggatcagc agcagagaac cccccgccac gccggatgcg ccacaggcac
     9721 acagagaagc caccacgctc aacagcagcg ccgtgggcag atcgaccggg atacccagcg
     9781 tattaaccgc agccagcgtc aacacggtaa tggtgattgc tgcgcccgcc atattgatgg
     9841 tggcacccag cggaatagaa acggaatagg tatcgcgatc cagattcagc ttttcacaca
     9901 gtgccatatt caccggaatg ttcgccgcag agctgcgggt gaagaaggca tacacgccgc
     9961 tttcgcgcag gcacagcagc accagcggga acgggttacg acgaattttc caccacacca
    10021 gcaatgggtt aaccaccagc gccaccagta acatacagcc aaccagcacg accagcagtt
    10081 gcgcgtagcc ccacagtgtg gagaaaccgg tggttgccag ggtagaagaa accagaccaa
    10141 aaataccaat cggtgcgaag cgaatgacca gtttcaccat aaaggtgacg gcattcgaca
    10201 tatcgttgac caggtttttg gtggtctcgt taccgtgacg cagtgcgaaa ccgaggccaa
    10261 ttgcccacac caggatcccg atgtagttac ctttcagcag cgcgtcgatg gggttggaaa
    10321 ccatgctcat taccagcccg cgcatcactt ctacaatgcc tgacggtggc gaaatatcac
    10381 ccgcgctact ggataagtgc agggtagaag ggaaggcgaa gctgaaaacg actgcggcca
    10441 gagcagcaga gaaggtaccc agcagataga ggaacaaaat agggcggata ttggttttct
    10501 gcccatgctg gtggttagca atagatgcca tcaccagcat caacaccagg atgggggcaa
    10561 cggctttcag tgcgccgacg aacaaagtac ctaacagacc aacagcttcc gccgcgggtt
    10621 ttgatatcca tgccagaaga atccccagaa caaggccgac caggatttgt tttaccaggc
    10681 tgccatgagc cagacgccgg aatagccccg gtgaacgttg cgtagtcatt tttcgatcct
    10741 ttcattgtgt tatcgcacat ccctggtgcg ttctgtcgac gcatctttca ggatgtaaca
    10801 aagtgtttgc aaggtcgagt ataaggaaag ttacagcttc gggaagcgaa aaatgctgga
    10861 ttttaaagag aagtcatatt ttgaaactta atggttacat ttgtttgaca taaacagagg
    10921 gaaggactgg ctactaaggc tgattagata acatatgggc aaaactattt ggaatatgag
    10981 aacggttgcg ctcctttaat gtgctatgcc atataaagtc actggcgcag ggagcgcgca
    11041 gggggcggcc aatcgccgcc gcccc
//
LOCUS       DAJVQG010000129        11049 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJVQG010000129 DAJVQG010000000
VERSION     DAJVQG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11049
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.129"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            28..153
                     /locus_tag="OQM83_003110"
     ncRNA           28..153
                     /ncRNA_class="other"
                     /locus_tag="OQM83_003110"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            273..614
                     /locus_tag="OQM83_003111"
     CDS             273..614
                     /locus_tag="OQM83_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCS1521309.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(605..1531)
                     /locus_tag="OQM83_003112"
     CDS             complement(605..1531)
                     /locus_tag="OQM83_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1521310.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGAPLYQI"
     gene            1621..2619
                     /locus_tag="OQM83_003113"
     CDS             1621..2619
                     /locus_tag="OQM83_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCS1521311.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2616..2834)
                     /locus_tag="OQM83_003114"
     CDS             complement(2616..2834)
                     /locus_tag="OQM83_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCS1521312.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2836..4851)
                     /gene="ligA"
                     /locus_tag="OQM83_003115"
     CDS             complement(2836..4851)
                     /gene="ligA"
                     /locus_tag="OQM83_003115"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCS1521313.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4922..5908)
                     /gene="zipA"
                     /locus_tag="OQM83_003116"
     CDS             complement(4922..5908)
                     /gene="zipA"
                     /locus_tag="OQM83_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCS1521314.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6138..6899
                     /gene="cysZ"
                     /locus_tag="OQM83_003117"
     CDS             6138..6899
                     /gene="cysZ"
                     /locus_tag="OQM83_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCS1521315.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7084..8055
                     /gene="cysK"
                     /locus_tag="OQM83_003118"
     CDS             7084..8055
                     /gene="cysK"
                     /locus_tag="OQM83_003118"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCS1521316.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8439..8696
                     /gene="ptsH"
                     /locus_tag="OQM83_003119"
     CDS             8439..8696
                     /gene="ptsH"
                     /locus_tag="OQM83_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCS1521317.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8741..10468
                     /gene="ptsI"
                     /locus_tag="OQM83_003120"
     CDS             8741..10468
                     /gene="ptsI"
                     /locus_tag="OQM83_003120"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCS1521318.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10509..11018
                     /gene="crr"
                     /locus_tag="OQM83_003121"
     CDS             10509..11018
                     /gene="crr"
                     /locus_tag="OQM83_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCS1521319.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         2692 a         2851 c         2745 g         2761 t
ORIGIN      
        1 taaaaaagcg ccctaaaggc gcttttttgc tatctgcgat actcaaagat tcgaacctgc
       61 agcaggtttg agttgagcgc agcgaaacaa cggagccgct cgcggcgacg gcccgaaggg
      121 cgagcgaagc gagtcatcct gcacgaccca ccaatgtaaa aaagcgccct aaaggcgctt
      181 ttttgctatc tgcgatttgt aagattgatt tttcttctca agttccttgt tcttctgccg
      241 atattacctc gtacagtgac gaggactgaa caatggccac tatctcttca acttcaattc
      301 cttccataca aacacagagt agtaacaggg catcacaagg tagcgatgtt gcttcaaaaa
      361 tcgcgcggat aagtcagcaa atcatcaaac tcactcaaca aattaaagag attgtagaca
      421 ctagcggcag tgctgaagac aaacaaaaac aggccgaatt gattcaacag caaatcaccc
      481 tactggaaac acaactggcg cagttgcaga gacagcaggc agaaaaggcg caggagaaag
      541 agcagcgttt gtctctgaat gtatcccttc taaatcctgt tgaaaatacg actcatatcg
      601 atatctatat ctgatacaac ggagcccctt cccgaccagc cattagcgca ttgccctggt
      661 ctcgtaccac gtcccataac gcttcagcgg cggtggaaag tgaacggttc ttcctgcgca
      721 ccagcatcaa ctggcgttca acaaccggcg taatgcgctt taccaccagt gggctacctt
      781 caggcaatgg tagcgccagt gcgggaagaa tactgatgcc gatgcctgcg gctaccatcg
      841 ggaacagcgt cgccggatgt ccaatctcct gcacaatatt cgcctgaata ccattgcgcg
      901 ccagcgctgc gtcaatcagc ggtcggctgc ctgacgcgta atcctgcaac accaatttcg
      961 ctccctgtaa tgcctgccag ggtacataat cttccacggc caaagcgcta tcgcgatggc
     1021 aaagcagaaa gaaaggctcg gaaagaatcg cttcacattg cagatcgccc actgggcctg
     1081 gatcgatgac gatgcaaaaa tcgacatctc cctggcgaat actttccatc acccactgct
     1141 gcggacgatc gtgcaggaca aactgaatat ctggatagcg gcgatggctt tcagcaatgc
     1201 attgcggaat aagatgcgcg gaaatggtct ggctggcagc gacccgaact tttccgctca
     1261 gttgttgccc catacgcccg gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct
     1321 caagacgcaa agccaactgc tgccctgcat ctgtaagcac cacttcacgc gtggttctgt
     1381 ccagcaggcg aacaccagta tgattttcca gctccttcac actgtgactt actgccgact
     1441 ggctcaagcc gatacgctct cctgcacgac taaaactttt ctcctgcgct actgtgacga
     1501 aaacctttaa ttgttttaaa gaataattca tctgttttct tcatgaatgg atgcaataaa
     1561 tcaattttat ttctcaaact ggaagaagca caatagaacc atcgatcatc tggagtcttt
     1621 atgaaacttt ttcgtatcct cgatcctttc accttaaccc tgatcacggt ggtgttgctg
     1681 gcctctttct ttccggccag aggcgatttc gtccccttct ttgaaaatct gaccaccgca
     1741 gctattgccc tgctgttctt tatgcacggc gcgaagttgt cgcgtgaggc gattattgct
     1801 ggcggtggtc actggcgact gcatttgtgg gtaatgtgca gcaccttcgt gctgtttccg
     1861 attctgggtg tactgtttgc ctggtggaaa ccggtaaatg tcgacccgat gctctactcc
     1921 ggttttctct acttgtgcat tctcccggct accgtgcagt ctgcaatcgc cttcacgtca
     1981 atggcgggcg gtaacgtcgc ggcggcggtt tgttctgcgt cagcatccag cctgctgggg
     2041 attttccttt caccattgct agttggtctg gtgatgaatg ttcacggtgc agggggcagc
     2101 cttgagcagg tcggtaaaat tatgctgcaa ctgctgctgc cgtttgtgtt ggggcatctt
     2161 tcccggccgt ggattggtga ctgggtgtcg cgcaataaaa aatggattgc gaaaactgac
     2221 cagacgtcca ttctgttggt ggtttataca gcgttcagcg aagccgtcgt taatggtatc
     2281 tggcataaag ttggctgggg atcattgctg tttatcgtgg tggtcagctg cgttcttctg
     2341 gctatcgtga ttgtagttaa cgtctttatg gcacgccgac tgggcttcaa taaggcagat
     2401 gaaattacta tcgtcttttg tggttcgaaa aagagtctgg caaatggcat cccgatggca
     2461 aacattctgt tccccacatc ggtgatcggt atgatggtgc tgcccctgat gattttccat
     2521 cagatccaat tgatggtctg tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag
     2581 ttacaggcgc agcaggaaag cagcgccgat aaagcttaaa gcggacgctt caggggctgg
     2641 accagttgcg tcagcccctc ggttttaatc agcagcgtga tttgcattag ctcaccgagc
     2701 ttccctgccg ggaactcatc tttgcgggca aaccacagca gatactcctc cggcaggtcg
     2761 attaagcgac gccctttgta tttgccaaac ggcattatcg tattggctat ttcaatcagc
     2821 tgctcttttt ccatctcagc tacccagcaa acgcagcatt tccgcttcgt cgatgacttc
     2881 aatgcccagt tcctgcgcct tcgccagttt agatcctgca gcttcacccg ctatcaccag
     2941 atcggttttc ttcgacacgc tgcccgcgac tttcgcgccc agttcgacca gtcgagcttt
     3001 agcgtcatca cgcgacatct ggcttaagct gcccgtaagc accacggttt taccagcaaa
     3061 cgggctgtca atctcttccg cgttgataac gatcggcgca ggccagtgaa caccttccgc
     3121 caacagctcg ctgatgacat tgcggttgct ttcttcggca aagaagttgt gaacgtggga
     3181 tgcaacgaca atgccaacat caggcacctt ttgcagctct tcaatcgaag cggcttccag
     3241 cacttccagc gtgccgaaat atgccgccag acctgctgcg gtggcctcgc cgacttcacg
     3301 gatgccaagt gcatagagga agcgagcaaa ggtggtttct ttcgcttttt ccagcgcgtt
     3361 aaccacgttt tgtgccgatt ttggccccat acgctccagt ccggtcagtt tgcctgcggt
     3421 gagtttgaac agatctgccg gagtgtggac atattctttt tcaaccagct gatcgatgat
     3481 tttgtcgccc attccgtcaa catccatcgc acggcgggaa acaaagtgtt tcagcgactc
     3541 tttacgctgc gcaccgcaaa tcaggccacc ggtacagcgg gcaaccgctt caccttccac
     3601 acgctcaacg tcagaaccac ataccggaca atgcgtcggg aatacaacct cacgggtatc
     3661 ttccgggcgt tcagaaagca cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg
     3721 aatcaccact ttatcgccaa tgcgtaaacc aagacgttcg atttcatccg cattgtgtaa
     3781 ggttgcgtta ctcaccagca cgccagcaac atggacaggt tccagacgcg caacaggcgt
     3841 aatcgcgcca gtacgcccaa cctgaaactc gacgtcacgc acaaaggtca tctgctcctg
     3901 cgccgggaat ttaaacgcta ccgcccagcg cggggcacgc gcgacaaagc caagctgctc
     3961 ctgctgttcc agtgagttga ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg
     4021 gcggtcttct tccactttgt gatagaacgc cagcacttct tccgccgatt cacaaagcgt
     4081 tacccgatcg ctgaccggca acccccactt tttaaattgc agtaaacggc caagatgagt
     4141 atccggcagc tcgccacctt ccagaacacc aacgccatag cagaaaaaag tgagcggtcg
     4201 cttcgctgta atacgcggat caagctgacg cagtgaacca gctgccgcat tacgtgggtt
     4261 agcaaaaact ttcccgcccg tgcgtcgcgc atcttcgtta atcttttcga accccgcctg
     4321 cggcaggaac acttcaccac gcacttccag acgcgccggg atattctctc cgtgcagctt
     4381 cagcggaatg gcgcgaatag tacgcacatt agacgtgata tcttccccgg tggtgccatc
     4441 gccacgggtc gcggcactga ctaaaacgcc attttcatac agaatactga cggcaaggcc
     4501 atccagcttc agctcacagc accaggtgac tttctcgttg tttttcagac ggtcctgcac
     4561 acgtttgttg aaagcaagaa agctttcttc atcaaaaacg ttatccagtg acagcattgg
     4621 tacttcatgg cgtatctggc tgaaagccgc cagcggcgca gcacctacac gttgggtagg
     4681 cgaatcaggc gtaatcagtt ctggatgttt ggtttccagc tcgcgcagtt cgcgcatcag
     4741 cctgtcgtat tcagcgtcgg gaatttccgg cgcatccatc acatgataaa gatattcatg
     4801 atggcgaagc gtcgttcgca gttctgtcag ttgttgttcg attgattcca tatcgcacca
     4861 tcaatgctaa aaacccccga caagcggggg ttcgaagagg agttaatttg ccttaagtgt
     4921 atcaggcgtt ggcgtctttg acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg
     4981 gagtcatcat acggcgctga tcgtcaagca cgacaccgcc cacttcatcg gcaatatgct
     5041 gcgcagattg cagcatcagc ttgaagttct gcagctcgtc accgtaagac ggtacctgca
     5101 taaagatagt gacacccgga gtagtgaaat ccttcatttc aggatcaaag gttcccggtt
     5161 tcaccatatt cgccaggctg aataacgccg ggccgctgcc atccgggcta agatgacgat
     5221 ggtaaatatt catatcgcca aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt
     5281 caccgtttag ctcgctaccg tgatgcgccg cgacgttcat gataatcacc gcttctttgc
     5341 gcttcggttt atccataact ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta
     5401 cgggttctgc aggctggaaa gcctgttgtg ccggttgcgg tgctgaatgc acaggctgcg
     5461 gcgctggcgc gacctgtggc gaaactggct gctgcaacgg ctgttcaggc tgcggctgat
     5521 aggcaggctg ctgcatcggc tgcgctggac gcggagcatg ttgcggcggt acctgcgctt
     5581 caggcggctg ctggatcggt tgacgcggct gcgcagacgc ataaggcggt tggtactggt
     5641 gttgcggcga cggacgagca gcctcatgct cctgagcgtt agccggggca tgattcacgc
     5701 ggtgaacacg aacctcacca acgccctcat catcttcgac atcctcgtca taagaatcgt
     5761 cgtcacgttt tgacttcatt cgttttaatg gccgatcgcg gaacatagaa gatcgttctt
     5821 tacggctggt ccagaaacca tgtaccagta aagcgattat ggcgatcgcg ccaacaatga
     5881 ttaatatcag acgcaaatcc tgcatcatta tattctctgt tgttctaaca ccttgccacc
     5941 acggcaaaca tttactcact aagagtattt gccgattacc tcaagtgcaa gtgcactatt
     6001 aactttcaca gcacaaagat agatgaaatc gtgctttttg ctgttttttc gaacatatcc
     6061 taactgtcca ttgcgcaatt acccggtaaa atacgcagaa ttttcctggg attggtcaaa
     6121 aggagctcat cctgattatg gtttcatcat tcacatctgc cccacgcagc ggtttttact
     6181 attttgcgca aggctggaag ctcgtctcgc aacctgggat tcggcgtttc gttattttac
     6241 cgctgctggt caatattttg ttgatggggg gcgcattctg gtggctcttt acacagctcg
     6301 atgtctggat cccgactctc atgagttacg ttccggactg gctacaatgg ctgagttatc
     6361 tgttgtggcc tctggctgtc atctctgtgc tgttagtgtt tggctatttc ttctccacga
     6421 ttgctaactg gattgccgct ccgtttaacg gtttattggc tgaacaactg gaagcacgat
     6481 tgactggcgc tacaccgcca gataccggga ttttcggtat catgaaagat gtgccgcgaa
     6541 tcatgaaacg cgaatggcaa aaatttgcct ggtatctgcc gcgcgcaatt gtattactaa
     6601 ttctttactt catccctggt attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta
     6661 gcgcctggat gttagccatc cagtattgcg attacccctt cgataaccac aaagtgccgt
     6721 ttaaagagat gcgcaccgcc ctgcgcacac gcaaaatcac caatatgcag tttggtgctt
     6781 taaccagcct gtttacgatg atcccgctgc ttaatctgtt catcatgccc gttgccgttt
     6841 gtggcgcgac ggcgatgtgg gtcgattgct atcgcgataa acacgcgatg tggcggtaac
     6901 aatctaccgg ttattttgta aaccgtttgt gtgaaacagg ggtggcttat gccgcccctt
     6961 attccatctt gcatgtcatt atttcccttc tgtatataga tatgctaaat ccttacttcc
     7021 gcatattctc tgagcgggta tgctacctgt tgtatcccaa tttcatacag ttaaggacag
     7081 gccatgagta agatttttga agataactcg ctgactatcg gtcacacgcc gctggttcgc
     7141 ctgaatcgca tcggtaacgg acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc
     7201 agcgttaagt gccgtatcgg tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg
     7261 aaaccaggcg ttgaactggt tgaaccgacc agcggtaata ccgggattgc actggcctat
     7321 gtagctgccg ctcgcggtta caaactcacc ctgaccatgc cagaaaccat gagtattgaa
     7381 cgccgcaagc tgctgaaagc gttaggtgca aacctggtgc tgacggaagg tgctaaaggc
     7441 atgaaaggcg caatccaaaa agcagaagaa attgtcgcca gcaatccaga gaaatacctg
     7501 ctgctgcaac aattcagcaa tccggcaaac cctgaaattc acgaaaaaac caccggtccg
     7561 gagatctggg aagataccga cggtcaggtt gatgtattta ttgctggcgt tgggactggc
     7621 ggtacgctga ctggcgtcag ccgctacatt aaaggcacca aaggcaagac cgatcttatc
     7681 tctgtcgccg ttgagccaac cgattctcca gttatcgccc aggcgctggc aggtgaagag
     7741 attaaacctg gcccgcataa aattcagggt attggcgctg gttttatccc ggctaacctc
     7801 gatctcaagc tggtcgataa agtcattggc atcaccaatg aagaagccat ttctaccgcc
     7861 cgtcgtctaa tggaagaaga aggtattctt gcaggtatct cttctggagc agctgttgcc
     7921 gcggcgttga aactacaaga agatgaaagc tttaccaata agaatattgt ggttattcta
     7981 ccatcatcgg gtgagcgtta tttaagcacc gcattgtttg ccgatctctt cactgagaaa
     8041 gaattgcaac agtaatgcca gcttgttaaa aatgcgtaaa aaagcacctt tttaggtgct
     8101 tttttgtggc ctgcttcaaa ctttcgcccc tcctggcatt gattcagcct gtcggaactg
     8161 gtatttaacc agactaatta ttttgatgcg cgaaattaat cgttacagga aaagccaaag
     8221 ctgaatcgat tttatgattt ggttcaattc ttcctttaac ggcataatgt ttaatgacgt
     8281 acgaaacgtc agcggtcaac acccgccagc aatggactgt attgcgctct tcgtgcgtcg
     8341 cgtctgttaa aaactggcgc taacaataca ggctaaagtc gaaccgccag gctagacttt
     8401 agttccacaa cactaaacct ataagttggg gaaatacaat gttccagcaa gaagttacca
     8461 ttaccgctcc gaacggtctg cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg
     8521 gcttcacttc tgaaattact gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt
     8581 ttaaactgca gactctgggc ctgactcaag gtaccgttgt gactatctcc gcagaaggcg
     8641 aagacgagca gaaagcggtt gaacatctgg ttaaactgat ggcggaactc gagtaatttc
     8701 ccgggttctt ttaaaaatca gtcacaagta aggtagggtt atgatttcag gcattttagc
     8761 atccccgggt atcgctttcg gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga
     8821 ccggaaaaaa atttctgccg accaggttga tcaggaagtt gaacgttttc tgagcggtcg
     8881 tgccaaggca tcagcccagc tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga
     8941 agaaaaagaa gccatctttg aagggcatat tatgctgctc gaagatgagg agctggagca
     9001 ggaaatcata gccctgatta aagataaaca catgacagct gacgcagctg ctcatgaagt
     9061 tatcgaaggt caggcttctg ccctggaaga gctggatgat gaatacctga aagaacgtgc
     9121 ggctgacgta cgtgatatcg gtaagcgcct gctgcgcaac atcctgggcc tgaagattat
     9181 cgacctgagc gccattcagg atgaagtcat tctggttgcc gctgacctga cgccgtccga
     9241 aaccgcacag ctgaacctga agaaggtgct gggtttcatc accgacgcgg gtggccgtac
     9301 ttcccacacc tctatcatgg cgcgttctct ggaactgcct gctatcgtgg gtaccggtag
     9361 cgtcacctct caggtgaaaa atgacgacta tctgattctg gatgccgtaa ataatcaggt
     9421 ttacgtcaat ccaaccaacg aagttattga taaaatgcgc gctgttcagg agcaagtggc
     9481 ttctgaaaaa gcagagcttg ctaaactgaa agatctgcca gctattacgc tggacggtca
     9541 ccaggtagaa gtatgcgcta acattggtac ggttcgtgac gttgaaggtg cagagcgtaa
     9601 cggcgctgaa ggcgttggtc tgtatcgtac tgagttcctg ttcatggacc gcgacgcgct
     9661 gcccactgaa gaagaacagt ttgctgctta caaagcagtg gctgaagcgt gtggctcgca
     9721 ggcggttatc gttcgtacca tggacatcgg cggcgacaaa gagctgccat acatgaactt
     9781 cccgaaagaa gagaacccgt tcctcggctg gcgcgctatc cgtatcgcga tggatcgtaa
     9841 agagatcctg cgcgatcagc tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg
     9901 cattatgttc ccgatgatca tctctgttga agaagtgcgt gcactgcgca aagagatcga
     9961 aatctacaaa caggaactgc gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg
    10021 cgtaatggtg gaaacaccgg ctgccgcaac aattgcacgt catttagcca aagaagttga
    10081 tttctttagt atcggcacca atgatttaac gcagtacact ctggcagttg accgtggtaa
    10141 tgatatgatt tcacaccttt accagccaat gtcaccgtcc gtgctgaact tgatcaagca
    10201 agttattgat gcttctcatg ctgaaggcaa atggactggc atgtgtggtg agcttgctgg
    10261 cgatgaacgt gctacacttc tgttgctggg gatgggtctg gacgaattct ctatgagcgc
    10321 catttctatc ccgcgcatta agaagattat ccgtaacacg aacttcgaag atgcgaaggt
    10381 gttagcagag caggctcttg ctcaaccgac aacggacgag ttaatgacgc tggttaacaa
    10441 gttcattgaa gaaaaaacaa tctgctaatc cacgagatgc ggcccaattt actgcttagg
    10501 agaagatcat gggtttgttc gataaactga aatctctggt ttccgacgac aagaaggata
    10561 ccggaactat tgagatcatt gctccgctct ctggcgagat cgtcaatatc gaagacgtgc
    10621 cggatgtcgt ttttgcggaa aaaatcgttg gtgatggtat tgctatcaaa ccaacgggta
    10681 acaaaatggt cgcgcctgta gacggtacca ttggtaaaat ctttgaaacc aatcacgcgt
    10741 tctctatcga atctgatagc ggcgttgaac tgttcgtcca cttcggtatc gacaccgttg
    10801 aactgaaagg cgaaggcttc aagcgtattg ctgaagaagg tcagcgcgtg aaagttggcg
    10861 atactgtcat tgaatttgat ctgccgctgc tggaagagaa agccaagtct accctgactc
    10921 cggttgttat ctccaacatg gacgaaatca aagaactgat caaactgtcc ggtagcgtaa
    10981 ccgtgggtga aaccccggtt atccgcatca agaagtaatt cttgccgcag tgaaaaatgg
    11041 cgcccatcg
//
LOCUS       DAJVQG010000130        11000 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJVQG010000130 DAJVQG010000000
VERSION     DAJVQG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11000
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.130"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..277)
                     /locus_tag="OQM83_003122"
     CDS             complement(<1..277)
                     /locus_tag="OQM83_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1521320.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRS"
     gene            complement(444..1184)
                     /locus_tag="OQM83_003123"
     CDS             complement(444..1184)
                     /locus_tag="OQM83_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCS1521321.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1277..1912)
                     /gene="argO"
                     /locus_tag="OQM83_003124"
     CDS             complement(1277..1912)
                     /gene="argO"
                     /locus_tag="OQM83_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCS1521322.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2051..2911)
                     /gene="mscS"
                     /locus_tag="OQM83_003125"
     CDS             complement(2051..2911)
                     /gene="mscS"
                     /locus_tag="OQM83_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCS1521323.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(3269..4348)
                     /gene="fbaA"
                     /locus_tag="OQM83_003126"
     CDS             complement(3269..4348)
                     /gene="fbaA"
                     /locus_tag="OQM83_003126"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCS1521324.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4563..5726)
                     /gene="pgk"
                     /locus_tag="OQM83_003127"
     CDS             complement(4563..5726)
                     /gene="pgk"
                     /locus_tag="OQM83_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCS1521325.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5776..6795)
                     /gene="epd"
                     /locus_tag="OQM83_003128"
     CDS             complement(5776..6795)
                     /gene="epd"
                     /locus_tag="OQM83_003128"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCS1521326.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7167..7598
                     /locus_tag="OQM83_003129"
     CDS             7167..7598
                     /locus_tag="OQM83_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1521327.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7621..8199
                     /locus_tag="OQM83_003130"
     CDS             7621..8199
                     /locus_tag="OQM83_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCS1521328.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8200..8907
                     /locus_tag="OQM83_003131"
     CDS             8200..8907
                     /locus_tag="OQM83_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCS1521329.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RQAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8895..9572
                     /locus_tag="OQM83_003132"
     CDS             8895..9572
                     /locus_tag="OQM83_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCS1521330.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9566..10243
                     /locus_tag="OQM83_003133"
     CDS             9566..10243
                     /locus_tag="OQM83_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS1521331.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10215..10928)
                     /locus_tag="OQM83_003134"
     CDS             complement(10215..10928)
                     /locus_tag="OQM83_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCS1521332.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10925..>11000)
                     /locus_tag="OQM83_003135"
     CDS             complement(10925..>11000)
                     /locus_tag="OQM83_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1521333.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2627 a         2918 c         2740 g         2715 t
ORIGIN      
        1 gtgaacgacg ccctgagtta tggatttcat tatcaatcgc agaaagtcgt tgataaaccg
       61 ggaacagttt ttgatacagt tcctgaccgg ctttattgag gctgatatta ttatctttgc
      121 gggtaaatag cgtatagcca atttgttctt ccagcgcggc aatgctttta ccaaatggcg
      181 aagccgtcat atggattttt tctgccgccc gggcaatatt attggtttgc gctaataaaa
      241 tgaaattgcg catctttttc gaaataaaaa tgtccatccc ccctccccgc atccttgtcc
      301 gcttaaccgt ttcagtcagc ctatccttga tgaaaccgcg agcaaagata ggtgattacg
      361 tcatggtttt acagaaaatt acagaaaaag gaggcaatat cgggtaaagg cattagcccg
      421 acgaatacgt cgggctacaa gtattattgt gctgcaggtg ttttagcggg ttgttgatcc
      481 acaggttcta actggaagac cacatcgacc tgatcatcaa actgaatagc ggcctgctcg
      541 taagtttcct gggcagacac cggcgcggca tcggctttca tcatccgcac cattgggctg
      601 ggctgatagt tggaaacatg gtagcgcacg ctatataccg gccccagttt acgatgaaag
      661 ccgttcgcca gttcctgcgc ctgatgaatc gcgttatcaa tcgctgcctt acgcgctttg
      721 tctttatagg catccggctg cgccacgccc agcgacacag aacgaatttc gttaagaccc
      781 gccttcagcg cgccatccag caaggaattc agtttgtcta actgacggag cgtgacttcc
      841 accgttctca cagcgcggta gcctttcagg atacttttac catcctgata atcataatct
      901 ggctgggtgc gtaagttcgc tgagctgata tcttttttcg cgatctgatt gagttcaagg
      961 aaggaaatgt attgtgcgac gcgctcatct gcctgtttct tggctgtagc ggcatccttc
     1021 gcggccacgt taacttcaat cgcaagagtg gcaatgtctg gcaccgcatc cacacttgcc
     1081 gtaccggagg tgacaatatg cggtccatcc ggcaattcgt tagcctgcgc tgccatcccg
     1141 ctaataccca ttaatgccgc cagggcgata actttgaact tcacgttagt ctctccgtgt
     1201 taattgactt gcccaaaatt caggacctct ggaggcaagc ttagcgcctc tgttttattt
     1261 ttccatcaga tagtgcctaa ctgaacaagg cttgtgcatg agcaataccg tctctcgcca
     1321 gctgcaaggc tataaaccac atgacacatc ccactaccag attgataatg cgctgtgatt
     1381 ttgccgtgcg caggcgcggt gccagccagg ctgcgagaat agccagacca aagaaccaca
     1441 ggaaagaggc gctaattgtc ccgagcgcaa accaacgttt tggttcgacg tcaagctgcc
     1501 cgccaaggct gcccagtaca acaaaagtat ccaggtaaac atgcggattc agccaggtca
     1561 ctgccagcat ggtggcgata attttccatc tgccttgctt caggacttcg gcactagcta
     1621 actcaatatt actactcatc gctgttttaa aagcgccaaa accataccac agcaagaagg
     1681 ctacgccgcc ccaggtgacc agcgccagca accacggcga ctgcatcaat aacgcgctgc
     1741 caccaaaaat cccggcgcaa atcaggacca aatcgctgat agcacaaagt aaggcaatca
     1801 taatgtggta ctgacgacga atgccctgat tcatcacaaa agcattttgt ggaccgagcg
     1861 gcagaatcat agctgcccca agagcaatac cttgaaagta ataagaaaac acgttggata
     1921 ttccgaatta agtatctgat gcagagacta tatcgcggga ttgttattag cggaaattga
     1981 taatattaat tggcaatcag aataactaat aagagagagg ccagagagta ttatctggcc
     2041 tcagcgttga ttacgcagct ttgtcttctt tcacccgctt aaagttcaca tccatttgcg
     2101 ggtacgggaa gctgataccg gcggcatcaa attcacgttt aatacgctcc agcacatccc
     2161 agtacacgtt ttgcagatcg ccgctgttgc tccagacgcg gaccacgaaa ttaatcgacg
     2221 atgcaccaag ttcgttcaga cgcacagtca tttcgcgatc tttcaaaatg cgatcttcag
     2281 actggataat attggtcagg atctgcttaa cctgatcgat atcggaatca tacgccacgc
     2341 caataataaa ttcgttacgg cgaactggct cgcgagagaa gttaataata tttccggcaa
     2401 taattttacc gttcggaata acgataattt taccgtctgc agtacgcatg gtggtggaga
     2461 aaatctgcac actcagcaca gtaccggcta cgccgccgag atcaacatat tctccggcac
     2521 ggaacgggcg gaacatgaca agtaacacgc cagcggccag gttagaaagt gacccctgca
     2581 aagccagacc aacagctaag cctgcggcac cgagtacagc aatgactgac gctgtttgta
     2641 cacccacgcg tcccagggca gcgattagcg taaaggcgat aataccgtaa cggactaatg
     2701 cagaaagaaa atcagcaaca gtggcatcga ttttacggga gatcatcagg cgattcaccg
     2761 cgttggaaat catccgcgcg ataatcaaac caacgatgat gatcgcgagt gccgccacga
     2821 tgtttactgc ataacttagc agcagcgcct ggttagctac cagccagctt cccgcgccgt
     2881 ttatgctatc gacaacattc aaatcttcca ttcaatattc cttttcaact gactcaaaat
     2941 ggagaaatac actccgccgc cttatgacgg gcagtctgac aagggtaaac aataaagcat
     3001 aattttgcca aatagatcac agatttctca tctgaatcac cttgcttttg gcgaaaaaat
     3061 ccccgcagag cggggctcag acagatatca atgtgcgctt tgtcatgccg gatgcggcgt
     3121 gaacgcctta tccggcctac aaaaacctgc aaattcaata tattgcagga tacgtgtagg
     3181 cctgataagc gtagcgcatc aggcaatttt gcttttggcg aaaaaaagac ccgcagagcg
     3241 ggccttgaga taagcagaaa ggaatatctt acagaacgtc gatcgcgttc agttcctgga
     3301 atgctttttc cagacgagcg atcatcgaag tctgaccggc acgcagccat acgcgcggat
     3361 cgtagtattt cttgttcggc tgatcttcgc ctttcgggtt acccaactgc ccctgcagat
     3421 aagcttcgtt cgctttgtag tagttcagaa cgccttccca ggttgcccat tgggtatcgg
     3481 tatcgatgtt catttttact acgccgtagc ttacggagtc tttgatttcc tgagcagtag
     3541 aaccggaacc accgtggaat acgaagttca ggctgttgtg cggcaggttg tgtttcttgg
     3601 aaacatattc ctgagaatca cgcaggatgg tcggagtcag aaccacgtta cccggcttgt
     3661 aaacaccgtg tacgttaccg aaggacgcag cgatggtgaa acgcgggctg attttgctca
     3721 gttcggtgta tgcgtaatca acgtcttccg gctgggtgta cagtgcagaa gcgtccatgt
     3781 ggctgttgtc cacgccgtct tcttcaccac cggtgcaacc cagttcgatt tccagagtca
     3841 tgccgatttt ggacatgcgc tccaggtatt tagagcagat ttcgatgttc tcttgcagag
     3901 attcttcaga caggtcgatc atgtgagaag agaacagcgg cttaccggta gctgcgaagt
     3961 gtttttcacc cgcgtccagc agaccgtcga tccacggcag cagtttcttc gcgcagtggt
     4021 cagtgtgcag gataaccgga acaccataat gttcagccat ctggtgaacg tgatgcgcac
     4081 cagagatcgc gcccaggata gcagcaccct gcggaacgtc agatttcacg cctttaccag
     4141 cgataaagga agcaccaccg ttggagaact gaacgataac cggcgcttta actttagcag
     4201 cggtttccag tacggcgttg atggagtcag taccgacgca gtttactgct ggcagtgcga
     4261 agttgttttc ttttgctacc tggaaaactt tctgtacgtc atcaccagtg attacgccag
     4321 gttttacgaa atcaaaaatc ttagacatgt ctcttgtcct gtatcgtcgg gccttatact
     4381 catcatactt caagttgcat gtgctgcgtc tgcgttcgct caccccagtc acttacttat
     4441 gtaagctcct ggggattcac tctcttgtcg ccttcctgca actcgaatta tttagagtat
     4501 gaaaaaatgc gtgctctaaa agcgcgctga aacaagggca ggtttccctg ccctgtgatt
     4561 ttttacttct tagcgcgctc ttcgagcatc gctactgcag gcagtacttt accttccacg
     4621 aattcgagga atgcgccgcc gccagtggag atgtaggaga ttttgtcagc aatgccgaac
     4681 aggtcgattg ctgccagagt gtcgccgccg ccagcgatgg agaacgcttc gctgtctgcg
     4741 atagcgttag ccacgatttc agtacctttg cggaagttcg ggaattcgaa cacgccaacc
     4801 ggaccgttcc acagaatggt tttcgcattc ttcaggattt cagccagttc ctgagcggaa
     4861 gcatcaccga tatccaggat ctgctcgtca gctttcacat cgttaacaga tttcagggta
     4921 gccgatgcag tttcagagaa ctcggttgct acgcgaacat cagacggaac cgggatgttg
     4981 caggtggtca gcagacgttt agcttcgtca accaggtcag cttcgtacag ggatttaccc
     5041 acatcgtggc cttgtgccgc gataaaggtg ttagcgatac caccaccaac aatcagctgg
     5101 tcagcgattt tagacaggga gtccagaacg gtcagtttgg tagatacttt agaaccacca
     5161 acgatagcca ccatcgggcg agcaggttct ttcagtgctt tacccagcgc gtccagttca
     5221 gctgccaaca gcgggcctgc gcacgcaaca tcagcgaatt taccgatacc gtgagtagaa
     5281 gcctgcgcgc ggtgagcagt accgaatgcg tccattacga acacgtcaca cagtgcagcg
     5341 tattttttgg acagggtttc gtcgtctttc ttctcgcctt tgttgaagcg aacgttttcc
     5401 agaacaacca gttcaccttc agcaacgtca acgccgtcga ggtaatcttt aaccagacga
     5461 accgggttag acagtttgtc tttcaggtag ttaacaaccg gcagcagaga gaattcttcg
     5521 ttgtactcgc cttcggtagg acgacccagg tgggaagtta ccatcacttt cgcgccttgt
     5581 ttcagggcca gttcgatggt cggcagagaa gcacggatac gcgcgtcgct ggttactttc
     5641 ccgtctttta ctggtacgtt cagatccgca cggataaata cacgtttccc agcaagatcc
     5701 agatcggtca tcttaattac agacatggtg aatcctctcg ttgattctaa aagttttgca
     5761 gacgctgctt gcgtcttacc tgaaagcaac agtagccata gctaacgtcg tgtcgagcat
     5821 tcggttagca aagccccatt cgttatcgca ccagaccaac gttttgatca ggtgtgcgcc
     5881 actgacccgg gtttgggtgc catcgacaat ggcactgtgc ggatcgtggt taaaatctac
     5941 agagaccaac ggcaattccg tatagtcaac tataccatga aatgcacctt gtgctgcttt
     6001 ttgcagcaac aggttgactt cattggcttt tacaggtttc ttcaccgtca cgcttaaatc
     6061 gattgccgtc acatttatgg ttggcacacg taccgcaatc gcttcaaagc gatcgttaaa
     6121 ttgcggaaaa aatcgtgtga taccggcggc cagtttagta tcgaccggaa tgatcgactg
     6181 gctggctgcc cgggtgcgac gcaggtcagg atggtatgca tcaataacct gttgatcgtg
     6241 catggcggag tgaattgtgg tcacagtgcc ggactcaata ccgtacgcat catctaacaa
     6301 tttgatgacg ggaattatgc aattcgtggt acaggaagcg ttagaaacga tgcggtgttc
     6361 cgcacgaagt tgatcctgat tgacgccgta aacaacggtc gcgtcgagat cgttactgcc
     6421 aggatgtgaa aagagcactt ttttggcccc ggcggcaata tgcgcttcgc catgctcgcg
     6481 ggagccatat acgccggtgc agtcgaggac tacatcaacg ccaagttcac gccaggggag
     6541 cgattgcagt gaacgttcat gcaatacgcg gatggcgtca tcaccaacaa aaagttgatc
     6601 gcgttcctgt cgtacttccc atgcaaaacg gccatggctg gtgtcatatt tcaacaaatg
     6661 cgccatgccc gcagcatccg ccagttcgtt gattgccacc acggtaattt ccgcccggcg
     6721 tccggattca tacaaagcac gaaccacatt acgcccgatg cgaccgaagc catttatcgc
     6781 tacgcgtacg gtcatagatc tcctgcaagg ttttccctga gcaaatttgc cagacagagt
     6841 aatccagcaa atcgtccggg gaaaccttac ctgtcgcaaa ctgcgactga ttggttaatt
     6901 gtcgaacatt taatcgactg aaacgcttca gctaggataa gcgaaacgtg gaataaaagg
     6961 aatgattgtc cagtcaaaga aaacaattat ctgacttacg tcacgttttc agccccgccc
     7021 ctgtggaatt tattccactt tgcagaatca cgaataagcg tagcaaaatc ttttcgttgt
     7081 gccgtcacaa accctgactt atatcagaat atgactgatg cagcgtcgat attatccgca
     7141 atacgtccga gtttcggagt actccaatga tgacattgcc ctacccatat tcttcccccg
     7201 cacgctttta tgcactgcgc ctcctccccg ggcaggaagt tttttcccag ctacacgctt
     7261 ttgcacagca gcatcaactt catgccgcct ggattgcagg ctgtaccggt agcctgactg
     7321 acgtcgcctt gcgctatgcc gggcaagaag gcaccacgct ccttaacggt acattcgaag
     7381 tgatctcact aaacggcacg ctggaacaga gtggtgagca tcttcatctc tgcgtttccg
     7441 atccacacgg cacgatgctt ggcggtcata tgatgcccgg ctgtaccgtg cggaccacgc
     7501 ttgaactggt gatcggtagc cttgaggaac tggcgtttag ccgccaacct tgcgcgcttt
     7561 ctggttacga cgagttgcat atttcccccg ttaaataact actttcaaag aggttttctt
     7621 atggcgcgta gccacttttc cagtcaggca ctggtgttga tcgttatatc tatcgccatc
     7681 aatatgatcg gcgggcaact ggccagcatg gtcaaattac ctatatttct cgactccatc
     7741 ggcacactta ttagcgcggt actgcttggt ccggtgatcg gaatgctgac cggcttactg
     7801 accaatttac tctggggatt gctgaccgac ccgatcgccg ccgcatttgc acccgtcgcg
     7861 atggtcatcg gcctggtcgc cggttggctg gcgcgagcgg gttggtttcg caccctgcca
     7921 aaagtcgttg tcagcggcgt gattattacg cttgcggtaa cggtcgtcgc tgtaccgctg
     7981 cgtactgcgc tgttcggcgg tgtcaccggt agcggagccg atttgttcgt tgcctggatg
     8041 cactcgatgg ggcaaaacct ggtggagtcg gtggcgatta cagtgatagg cgcgaacctg
     8101 gtcgataaaa ttcttacagc ggtgattgtc tggcttttac tgcgccagct gccgattcgc
     8161 accacgcgcc attttccggc aatggctgcc gtgcgctaaa tgcatccatt tacctcatta
     8221 acactgtggg cattggcggc ctgcaccacg cttatcctgc ccgcgcagac cattttgccg
     8281 atctatagcg cggcgacctt tttttgtctt attgcgctaa aggccacgcg gcggcaggca
     8341 aaatatgtcg cctggctgat gttctcgctg ggagccgggc tgtggctggt acacggtggc
     8401 tggctaacag aatggctaag cggcacgccg cgttcgccgg agcgttggac gcatgctatt
     8461 acgctctggt tgcggattct ggcgattgtt tcaacctcac agttgtggat gcagtacgtc
     8521 cccgtgcaat gttttatccg cgctctgttt gcttctcgcc tgccgccagg cgtcgcttat
     8581 ctgtttgccg gaccgttatt ggtcgttgag cagttaaaac ggcaattagc gattattcat
     8641 gaagcgcagc gtgcccgcgg cgtgccgctg gatgagggct ggtatcagcg tttacgtgcg
     8701 atgcccgcgt taattattct gctaacacac aatgcgctta acgatctggc tgtccgtggt
     8761 gcggcgctgg atatgcgggc atttcggatc cacaaccgac gaacaacgtt gtgggcacca
     8821 gccgatagca ccctacaacg cgtagcgcgt tacaccatga tcttgctgat gctaacagaa
     8881 ttcggagcgt ggatatggtt acgttagaac agttccgtta ttgcccgaca cactcaacgc
     8941 atcctccgtt ctgttatgac tttcactatg tcaaaccggg gatggtcgcc atctttggtg
     9001 acaacggcag tggcaaaagt acgctggcac agctgatggc gggctggtat ccggactttc
     9061 tgccaggaga gataacaggc acggggacgc tcctcggtac gcccattggg catctcccgc
     9121 ttaatgaaca atccgccacc atccagctcg tccagcaatc gccctatttg cagctctccg
     9181 gctgtacctt tagcgtggaa gaagaagttg cctttgggcc agaaaatctg tgtcttgctg
     9241 aaacagagat tatggcgcgg attgatgccg ccctgactct gaccgagtgc cagccgttgc
     9301 gccatcgtca cccggcaaca ctctccggtg gagagacaca acgcgtggtc atcgcctgcg
     9361 ccatcgcgat gcagccaaag ctcttaattc ttgatgaagc ctttagtcgc ctgacgccgc
     9421 aggccagcga aatgctgttg cagcgcttac aacactgggc gcttgaacgt ggttcgctga
     9481 ttattctttt tgaacgtcat cgcacacctt tcctgaacta ctgccagcaa gcgtggcaat
     9541 tgcaaaacgg agcgctccag ccactatgtt aacgttaaat cagatatctt atcgctggcc
     9601 tagcgcagca acggattgtc tttgcgacat ttctctgcaa ctcaaacaag gtgaatggtt
     9661 ggcgctgacc ggcgacaatg gcgcgggaaa atcgacgctg ttacgagtaa tggcaggtct
     9721 tcttacgccc acagccggta cggttatgct gcaacaacag gcgatgaaaa acctgaaaaa
     9781 ccgccagcgc gcggcaaagg ttggcgtact gtttcaggaa gcggaaaacc aactttttca
     9841 cagcaccgtc gctgacgaga tcgcatttgg tttgaaactg caaaaatgcc cggcggatga
     9901 aatcactcaa cgtacccatg ctgccctgca atgttgtcag ttagcagaca ctgccaactc
     9961 gcacccgttg gatctgcata gtgcgcagcg acggatggtc gccgtcgcct gcctggaagc
    10021 actctctccg ccactattac tgctggacga gccgagtcgg gattttgacg aaaattggtt
    10081 gagcgtgttt gaaagctggc tggagaagtg ccgccagcga ggcacgagcg tggtagcaat
    10141 cagtcatgac gccgcattta cccggcgtca tttctctcgt gtggtgcgac tggaagatgg
    10201 tgtaatcagg aacgttaatc caccagacga tattcacccg tagcggtcat cattaaggtc
    10261 aaatttgcag gcagactctc ttccagcacc cggcgaacat tcggcccgtc ggtacggtca
    10321 taaaaggctt ctgcatctgc cagcgatacc cctcccgcca gcttgcgccc caccagacgc
    10381 tcccgcaacg cagtagctgg cgcgttgata aagatcgaga aatcacaaaa ttgcgcaagc
    10441 tggcaccact tctcgtcgtc cagcaacaac cagtttcctt cgacgatgac gagtggtgcg
    10501 gtaacgtgca acgcatcttc aacaggatca tgcttttgtc gatcgtactg cggccacgta
    10561 caatcccctt ccaccacctg gcgcagattt tccgccagtt tcgcaacgtc aaatgtctct
    10621 ggtgcgcctt tgaaggggcg caattgatgc gcgtccagcc agctattgta atggtgaaaa
    10681 ccatccatcg ggagcgtctg aatagcgggc agctccgggt cttgttgcac gagatattcc
    10741 cagaaggtgg ttagggtaga tttacccgtc cccggcggag cgcaaagaaa aaccaccgta
    10801 cgccgctgcg gatttaccgt ctgtaaggcc gccagcatac gcagcaacgg tttatgcacg
    10861 cgttctatct cttcatcatg gtactgcgct tgcacgttca gcccattcac cgttaattca
    10921 attttcacgg cgttaattcc tttaaggtgg tcgttactgc cagcgtcagc gccgttttga
    10981 ccattccgct aaaacgcgcc
//
LOCUS       DAJVQG010000131        10930 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJVQG010000131 DAJVQG010000000
VERSION     DAJVQG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10930
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.131"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..312)
                     /locus_tag="OQM83_003136"
     CDS             complement(<1..312)
                     /locus_tag="OQM83_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCS1521334.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LA"
     gene            complement(331..2208)
                     /gene="bglF"
                     /locus_tag="OQM83_003137"
     CDS             complement(331..2208)
                     /gene="bglF"
                     /locus_tag="OQM83_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCS1521335.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="OQM83_003138"
     CDS             complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="OQM83_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCS1521336.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="OQM83_003139"
     CDS             complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="OQM83_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCS1521337.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="OQM83_003140"
     CDS             complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="OQM83_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCS1521338.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="OQM83_003141"
     CDS             complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="OQM83_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCS1521339.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSVPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="OQM83_003142"
     CDS             complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="OQM83_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCS1521340.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="OQM83_003143"
     CDS             complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="OQM83_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCS1521341.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDSPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTTFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8292..9365)
                     /locus_tag="OQM83_003144"
     CDS             complement(8292..9365)
                     /locus_tag="OQM83_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1521342.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(9376..>10930)
                     /locus_tag="OQM83_003145"
     CDS             complement(9376..>10930)
                     /locus_tag="OQM83_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1521343.1"
                     /translation="VTIKEEDGSEQRFTQPYASLAILKREGQTDVDVSVGELRDEDGF
                     TPDVLQAQILHGFSHGITLYGGMQAAENYGSAALGVGKDLGALGAISFDVTHARANFS
                     HDDTETGQSYRFLYSKRFDDTDTSLRLVGYRYSTEGYYTLNEWASRRNSPEDFWETGN
                     RRSCVEGTLTQSLGRDYGNLYLTLSRQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSY
                     SNTARQGTGNNHASDNTSEQIYMLSLSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLS
                     GTALERNNLSWNLMQSYNSHDDEVGGNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRG
                     GILAHSEGVTLSQELGETIALVKAPGAAGLEIDNMRGAATDWRGYTVKTQLNPYDENR
                     VAISDNYFSKSNIELDNTVVTMVPTRGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGA
                     IAAIQDASLADSGIVGDRGELYLSGLPEKGQVTLSWGENASTKCIFNYSLSTPESESG
                     LIEQGVTCH"
BASE COUNT         2851 a         2830 c         2480 g         2769 t
ORIGIN      
        1 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
       61 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
      121 acggtgataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
      181 catttttccc ataacgccat gaggctgtaa atctgaggtc gtgctccctt tgccatcttc
      241 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
      301 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
      361 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
      421 taataccggc gtcgtcacat caaatccggc ctcgcgaata gcagggatat caaaagaaat
      481 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
      541 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
      601 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
      661 cacttcacca accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
      721 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
      781 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
      841 acgtttagcg gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat
      901 gacaccgcca ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg
      961 catgaaggtg aaaatacttg gcaaaccaaa ggagtacact ttcgtttgcg cgtagccaat
     1021 gatggtggcc cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag
     1081 attaacgcca tataccgctg gttcggtgat accaaacagg cccgtcaacg ccgctgatcc
     1141 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     1201 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     1261 gttattgata cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca
     1321 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     1381 ggcggcaatc agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     1441 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     1501 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     1561 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc
     1621 cgctttttgc ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat
     1681 caccatggca gtaaatggat taccgccaaa gcgtttcccc gcggtgtatc ccaggattat
     1741 cgggaagaac caaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     1801 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     1861 cgccatcaaa ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     1921 cagattacct ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac
     1981 gctgttgacc gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc
     2041 accgctttcc accaccataa taataccggg ggtgtttttc agtacctcag cttgcgcttt
     2101 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt
     2161 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     2221 accgctttgc ggcaaaactc caaaaaaaaa actgaaaaaa acggcctgac gtgaatcaag
     2281 caattttttt caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat
     2341 ttcagtgttc tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg
     2401 aaattttacg ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg
     2461 cttgcgggta attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag
     2521 catgttcaag aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca
     2581 agctttcttc ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca
     2641 ttaactgcgt gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat
     2701 gcatggcaat aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa
     2761 tggttaacgc ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca
     2821 gcaacgggtt gggcagcaac acgttttgct gaaagcgttt aatcgcaaac tggcaatggt
     2881 cagttagcga gatataaata ctgtcctgta attttcccaa gcgctcctgc gctaaagaga
     2941 taatacgatc acaggttgcc atcacctcaa gaggaatatg acttaagagt tcgcttaatc
     3001 gcccgttcag ttcatgactg ctcaaggcat actctttttc tattccactt gagttaattc
     3061 tttcgccagc gcatttttga aagccaattc ctcgccccat gacgactttt tcccgctgtt
     3121 gatcatcaat aaccaccaca acattattgt tgagaatttt ggtgatttgc atgttcatag
     3181 caaggacctt ttttataaac aaaaaaaacc gacttcacca gtattctctg gttatgtcag
     3241 gttttgcctg cgaatgcagt aacaatccag tcatttatta atgtttttta taacgaacat
     3301 ccaggttcgg aaattaattt aattgcgtgc agagaaaata gcaatgcgct attgataaaa
     3361 atatgaccat gctcgcagtt attaactttg tgtaatttta ggaatttata aagttatata
     3421 taacaaatcc caataattaa gttattggga tttgtctggt gaattatttg tcgctatctt
     3481 ttcccgccag cagtttatcc agctcatcac cgcctacatg acggaaatcc tgccccttca
     3541 cgtagtagaa gataaactca caaatattct ggcagcggtc gccaatacgt tcgatagaac
     3601 gcgcgcagaa cagcgcagta agtacgctcg gaatggtacg cgaatcttcc atcatgtagg
     3661 tcatcagttg acgaacaata ccttcgtatt cctgatcgac ttttttatct tcacgataaa
     3721 tacgtaccgc ttcgtcaatg tccatccgcg cgaacgcgtc cagcacgtcg tgcagcatct
     3781 ggatggtatg acggcccagc gactccagac ttaccagcaa cggctgatgc tgctgggaga
     3841 atttctccag cgcagtacgg cagattttgt ccgccacgtc gccaatacgc tccagctcgg
     3901 caatggtttt actgatcacc ataaccagtc gcaggtcgct cgccgtcggc tgacgtttgg
     3961 cgataatgcg cacgcaggct tcatcgatcg ccacttccat catgttgacg ttcttgtcgc
     4021 cttcgatcac gcgcttcgcc agatcgctgt cctggttatg catcgcggtg attgcatcag
     4081 aaagctgctg ctccaccatg ccacccatgg tcatcacctg cgtgcggata ctttccagtt
     4141 cggcgttgaa ctggccggaa atatgtttat taagattgag actgtccata acgcactcct
     4201 gaattaaccg taacgaccgg tgatgtagtc ttctgtttgt ttcttcgctg gcttggtgaa
     4261 cagatcgtcc gtgttgctga actcaatcaa ttcgcccagg tacataaacg ccgtgtggtc
     4321 ggaacaacgc gcagcctgct gcatgttgtg ggtaacgatc accacggtgt aatcctgctt
     4381 cagttcggtg atcagctctt caatacgccc ggtagagatg gggtcgagcg ccgaacacgg
     4441 ttcgtcgagc agcagcactt ccgggcgaat ggcgatacca cgcgcaatac acagacgctg
     4501 ttgctgacca ccagagagag agtaaccgct ctggtgcaat ttatctttgg tttcgttcca
     4561 caatgcggct ttggtcaatg cccactgcac gcgctcgtcc atgtcggcac gggagagctt
     4621 ctcaaacaga cgaacaccaa aagcgatgtt gtcgtagatg gacatcggga acggcgtcgg
     4681 tttctggaac accatgccca ctttcgcacg cagcagtgcg atatcctgag agttggtcag
     4741 gatgttgtcg ccatcaagca gaatttcacc ttctgcgcgc tgctccggat acagttcaaa
     4801 cattttgttg aaggtacgca gcagcgtcga tttaccgcag ccggacggcc cgataaacgc
     4861 cgttacctgg tttttagcga tatccaggtt gatgtttttc agggcatgga atttgccgta
     4921 gtagaagttc aaattacgaa cctgaatttt actcggggca gtttcaacca tactcattgc
     4981 aatcttttcc tcatcgtacc tgatgctttt cgctaatcag gcttaccgta ggccgggtaa
     5041 ggcgttcacg ccgcatccgg caaagtgtta accgtgttta ttcttcgcaa aaacaacgcg
     5101 cgccagaatg ttcagcagca ggacgcacag ggtaatgatc aataccccgg cccaggccag
     5161 ttgttgccat tccgcaaacg ggctcatcgc aaacttaaag atcgtcaccg gcaggttggc
     5221 gatcggctgc atcatgtccg tgctccagaa ctggttggag agcgcggtaa acagcagcgg
     5281 cgcggtttca ccggcaatac gggcaatcgc cagcaggata ccggtcataa tcccggacac
     5341 cgacgctttc agcgtaatcg cggagatcat cttccacttc ggtgtaccca gcgcataagc
     5401 cgcttcacgc aggctgtccg gcaccagttt cagcatgttc tcggtggtgc ggataacgat
     5461 cggcacctgc aacagcgcca gcgcaattac gcccgcccag ccggagaagt gctccatctg
     5521 tgccaccacg atggtgtaga caaacagacc aaccacaatc gacggcacgg agagcagaat
     5581 gtcgttaatg aagcgaatca cttctgccag ccaggattta cgaccatatt ccgccagata
     5641 aatccccgcc ataatgccca gcggcgtacc gaataccgtg gcccacaaaa ttaacagccc
     5701 gctacccgcc agggcgttcg ccagaccacc accttccgta ttgggcggcg gcgtcatttc
     5761 agtgaacagc gccagcgaca taccgtcgat accgcgagtg atggtggaca ttaaaatcca
     5821 gatcagccag aacaggccga aggccatcgt cgccatcgag agcgtcagcg caatacggtt
     5881 tttgaggcgg cgacgcgcct gcattttgcg gcgagattca gccagcgccg cagtggtttg
     5941 catttcaacc atagccatta gcgtgccccc tcattcttag ccaggcgcat aatcataaac
     6001 ttcgatgcgg cgaggacgat gaaggtaatc acaaacagga tcaggcccag ttccatcagt
     6061 gcggcaacgt gcagaccgga ttccgcttcc gcaaattcgt tcgccaacgc agaggtgata
     6121 ctattgcccg gcatgtacag cgaggcgctg tcgagctggt aggtgttacc gatgataaag
     6181 gtcaccgcca tggtttcacc gagcgcgcgg cccagcccca gcatgatgcc gccgataaca
     6241 ccatttttgg tgaacggaag aacgatacgc cagataactt cccaggtggt gcagccaata
     6301 ccgtaggccg actctttcat catcaccggg gtttgttcga acacatcacg cattaccgcc
     6361 gcaatgtacg gaataatcat gatggcgagg atcacgcctg ccgcgaggat accgatacca
     6421 aatgcggggc cagagaacag cgcgccaaca atcgggatat tcgacatgat attgccgacc
     6481 ggctcctgaa agtaaacggc gaacagcggc gcaaagataa acaggcccca catgccgtaa
     6541 acgatacttg gaatggctgc cagcagctca atggcgatac ccagcgggcg tttcagccag
     6601 ccaggcgcaa gctcagtcag gaacagggcg ataccgaaac tcaccgggac ggcgatcagc
     6661 agcgcgataa acgaagtcac caacgtaccg tagatcggca ccagcgcccc gtagatatcg
     6721 ttcggtgcat cccactcttt ggtccatagg aaagccagac caaatttctg aatgctcggc
     6781 caggaggaga tgatcagaga gacaataatg ccacccaaca tcaatagcac aatcagcgcc
     6841 gccagtttta ccagcacgct gaaaattatg tcgccctttt tacccggtgg gttaaaagca
     6901 ggcttggttg cagccataag ttactcttca gttaaacgcg tttacaaagt cattttgtaa
     6961 tgccggatgc ggcgtaaaac accgtacccg gcctggagtt ttattagtac agcggcttac
     7021 cgctactgtc tttaatattg gtcttccacg cagcgcgaac ctgttcaact acactatccg
     7081 gcaggctggc gtaatccagg tcgttcgcct gtttagcccc ggttttgtac gcccagtcga
     7141 agaatttcag cacttctgtg ccttgttctg gtttcttctg atctttgtgg atcagaatga
     7201 acgtggtaga ggtaataggc catgcatctt cgcctttctg gttggtcaga tcctgagcga
     7261 aggttttgct ccagtctgca ccttttgctg cattagcgaa gttttcttcg gtcggactaa
     7321 ccggtttacc atcagcggag atcagtttgg tgtacgccag gttgttctgc ttcgcgtaag
     7381 catattcaac ataaccaatt gcacccggca gacgctgaac gaacgcggcg ataccgtcgt
     7441 tacctttacc gcccagaccg atcggccatt ttacggtaga gccagtacca acgttgtttt
     7501 tccactcttc gttcactttc gccaggtagc tggtgaagac gaaggtagtc ccggagccat
     7561 ctgcgcggcg tactacagca atgttttgtg aaggcagttt cagacccgga ttcagtttgg
     7621 cgatggtttc atcatcccac ttcttgattt tgcccaggta gatgtcgccg agggttttac
     7681 catccagcac cagttcgcca gacttcagcc ctggaatgtt aaccgccagc accacgccgc
     7741 caatcacggt cgggaactgg aacagacctt cctgagccag tttttcgtca gacagcggcg
     7801 agtcagaggc accaaaatca acggtattag cgataatctg ttttacgcca ccggaagaac
     7861 cgataccctg gtagttaact ttattaccga tttctttctg gtaagtgtca gcccatttgg
     7921 catacaccgg cgcagggaag gttgcacctg cacctgtcag gcttgcttct gcaaacacag
     7981 agaaagcgct catcgataag gtcgcggcga caacagttgc gacggtggta cgcataactt
     8041 tcataatgtc tcctgggagg attcataaag cattgtttgt tggctacgag aagcaaaata
     8101 ggacaaacag gtgacagtta tatgtaagga atatgacatt tttatgacag aaaggaatta
     8161 acaaataact tactgatttt aaatgtgaag atgaaaatcc cacatgtcat ctttttgtca
     8221 tgatgcattt aaaagtgata aagaaatggg tgagatgaca acgatgtgat gaacaatcgt
     8281 tgtcagacct attagcgcac aatctcaaca gttatcgtcg ctgttgcagt aaattgcccc
     8341 ggggctgggc gagctccagt aaggctcgca ggagcagctg aaaaagttac actacctgat
     8401 ttttcaccat acactgttga tttacccatg tctgttggca actcaccacc gttcactttg
     8461 atttccttat tatttttgtc gtaaattttt atccccacgt tagagtttga agttacgacc
     8521 attgaattgt ccgtattggg ttcggtagca taaaaagacg tactgatatc atcggtattc
     8581 agtatcccca ggttttcaca ctcaatagtg actgttttag taattttatg tgttgttacc
     8641 gcacttccaa caacagatga aaaatctgca gctggtattt ctttaaaatc aaaataaact
     8701 ggctgtaaat tatttatttc gcaactttgc ggagctgtaa tatcgccccg aatataaaca
     8761 tcagccatcg gctgtgagta atccaccggg gtgtcacggc taattgttcc atacaatgtc
     8821 actatctttg tttcaggaat aatcaatttt cctgcaaatg tcttcttgat ataaaaatat
     8881 actatcgctt tacccccact ggatatactt aaaggttcaa tgcggttgca attgtacttt
     8941 gtattaccag acgggttgtt agcatgatat tcaaaaggtg cattcacata cccaacccca
     9001 agaatatcta tagcaatccc aacatcaagg ttcggattaa gatcataata attaatatta
     9061 gagtgaaccc cagcataagt taatgcctgg tttatttttg acgtataata gatatagggg
     9121 aattctccta cattaccagt atcacaatga cagcgtccag gatatgagga tccattagtt
     9181 acagtggtat taaatgtcgt tccaacctta ttttcgttcg cggtaataag atatcccgag
     9241 aaatcaagcg tacggtgaaa tacgccatta tcaggggtac agataccttt aggagctgca
     9301 agtgctacac tacttaacga gagcataaac acagcaaagc accactgttt gatatacttg
     9361 ttcataaaaa tccttttaat gacatgtcac accctgttca attaatccgc tctcactttc
     9421 tggtgtcgaa agtgaataat tgaagatgca ttttgttgag gcgttttctc cccaggataa
     9481 cgtaacctgt cctttttctg gtagaccaga aagataaagt tcgccacggt caccgacaat
     9541 tcctgaatct gccaaacttg catcttgtat cgcagcaatg gctccaaaag gtaccggttt
     9601 accatttgca tttaacactc tgaagagaac gcgataaccc acatgagtca caaactccgc
     9661 tttaaccact gcaccacgcg tgggaaccat cgtaacgacg gtattatcaa gttctatatt
     9721 cgacttcgag aaatagttat cgctgattgc tacccgattt tcatcataag ggtttagctg
     9781 tgtcttgacc gtatagccgc gccagtccgt cgcagcaccg cgcatattat ctatttctaa
     9841 cccggcggcc ccaggtgctt taacaagagc aatagtttca cctaactcct gacttaacgt
     9901 taccccttcg ctgtgtgcca gaattccccc tctgatacca taattcagcc tctgggaatt
     9961 ttggctgtag ttatagctgc cgttcaccgt gccataagag ccatcatagg tcagggacat
    10021 attaccgcca acctcatcat catgactgtt ataggactgc attaaattcc atgaaaggtt
    10081 atttctttcc agcgccgtac cgctgagtcc gagttgatgt gagctaccgg aattatcgtt
    10141 ttgcgaaaca gaataggtgg cgtaactatt accccaccag cccaataaag gaacagataa
    10201 agagagcatg tagatctgct cactggtgtt atcactggca tggttgttcc ccgtcccctg
    10261 tctggcagta ttggaataac tccaggagac gttccacgag agacgcttcc agctactgct
    10321 gtagccaaat tgcattaatc gttcgacatc atcggtatgc cagtattgtt gccggcttaa
    10381 tgtcaggtat aaattgccat aatctctccc caacgactgc gttagcgtcc cctccacgca
    10441 actacgtcgg ttacctgttt cccaaaagtc ttcagggctg ttgcgccgcg atgcccactc
    10501 attgagggta tagtagccct cggtggagta acgatagcca accaagcgca agctagtgtc
    10561 tgtgtcgtca aatcgttttg aatagagaaa gcgatatgac tgacccgttt ctgtatcatc
    10621 atggctaaaa ttcgcacgag catgtgtcac atcgaaagaa attgcgccca aagcgccaag
    10681 atctttaccg acacccagag ctgcagaacc ataattttca gcagcctgca tacctccata
    10741 taaagtgatc ccgtgggaaa aaccatgaag tatttgcgcc tgaaggacgt ccggtgtaaa
    10801 tccgtcttca tcgcgcaatt cacccacgct gacatcaaca tctgtctgac cttcacgttt
    10861 aagaatcgct aatgaagcat aaggttgggt gaatcgttgt tcgctaccgt cttcttcttt
    10921 gatggttacg
//
LOCUS       DAJVQG010000132        10899 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJVQG010000132 DAJVQG010000000
VERSION     DAJVQG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10899
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.132"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            1..611
                     /locus_tag="OQM83_003146"
                     /pseudo
     CDS             1..611
                     /locus_tag="OQM83_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            608..1222
                     /locus_tag="OQM83_003147"
     CDS             608..1222
                     /locus_tag="OQM83_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCS1521344.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="OQM83_003148"
     CDS             complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="OQM83_003148"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCS1521345.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2997..3455
                     /gene="gpt"
                     /locus_tag="OQM83_003149"
     CDS             2997..3455
                     /gene="gpt"
                     /locus_tag="OQM83_003149"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCS1521346.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3547..4791
                     /gene="frsA"
                     /locus_tag="OQM83_003150"
     CDS             3547..4791
                     /gene="frsA"
                     /locus_tag="OQM83_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCS1521347.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4849..5250
                     /gene="crl"
                     /locus_tag="OQM83_003151"
     CDS             4849..5250
                     /gene="crl"
                     /locus_tag="OQM83_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCS1521348.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="OQM83_003152"
     CDS             complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="OQM83_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCS1521349.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6632..7735
                     /gene="proB"
                     /locus_tag="OQM83_003153"
     CDS             6632..7735
                     /gene="proB"
                     /locus_tag="OQM83_003153"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCS1521350.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGNSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7747..9000
                     /gene="proA"
                     /locus_tag="OQM83_003154"
     CDS             7747..9000
                     /gene="proA"
                     /locus_tag="OQM83_003154"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCS1521351.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9115..9190
                     /locus_tag="OQM83_003155"
     tRNA            9115..9190
                     /locus_tag="OQM83_003155"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9148..9150,aa:Thr,seq:cgt)
     gene            9796..9954
                     /locus_tag="OQM83_003156"
     CDS             9796..9954
                     /locus_tag="OQM83_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521352.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2761 a         2575 c         2778 g         2785 t
ORIGIN      
        1 ctattttaca gcggcatatt gcctcgtttt cgcatcatgg tttgcctgaa ggcagtgacg
       61 acgcgctacg gtatattgcg gaacatgacg atgcgctggc gtttgcgcgt attaatcgcc
      121 agctgatctc tttacgcata atgcaacagg ttaaggccac cggaagtccg gttctggatg
      181 tggcgcataa ctttgttagc gcgtgtcaaa tcggcgatca acagggctgg ttgcatcgta
      241 aaggtgccac gccggatgac aacggtctgg tgattattcc cggttcactc ggtgattact
      301 cctagcttgt acagcctgtt gcgaacgaaa aaacgctcca ctcgctggcg catggggctg
      361 ggcgtaaatg ggggcgcacc gagtgtaaag ggcgtctggc agcgaaatac acagcgacgc
      421 agctctcacg tactgaactt ggcagtcggg taatttgtcg cgataaacaa ctcatctttg
      481 aagaagcgcc acaagcctat aaatcggctg aaagcgtggt gcaatgtctg gtgcaggctg
      541 gtttaattat tcctgtcgcg cggctgcgtc cggtgctaac gctcaaaaac agtggaggga
      601 aaaaaggatg atcttgctac aactctcctc agcgcagggg ccggaagaat gttgtctcgc
      661 agtgaaaaag gcgctggagc gattaattaa agaagctgcc cgacaagacg tcgcggtaac
      721 ggtgttggaa acagaaacgg gccgctactc tgacacgctg cgttcagctc tggtttctct
      781 ggatggcgat aacgcatggg cattaagcga aagctggtgc ggcactattc agtggatttg
      841 tccgagtccg tatcggcctc atcatgggcg caaaaactgg tttctgggca ttgggcgttt
      901 taccgctgat gagcaggaac aatcggatgc aatccgttat gagacgttgc gttcgtcggg
      961 gccgggcggt caacatgtca ataaaaccga ctcggcggta cgcgccacgc atttggcatc
     1021 cggtattagc gtgaaggttc agtcagagcg tagtcagcat gctaacaagc ggctggcacg
     1081 attgctgatt gcctggaagc tggagcagca gcaacaggaa aatagcgcgg tgctgaaatc
     1141 gcagcggcga atgttccatc accagattga acgtggcaac ccgagacgga catttacagg
     1201 gatggctttt atcgaaggat aatgaaggat gaaactgccg gaaggcgatt aaacgccatc
     1261 cggcagcaaa tcaaataatt acttcgccgg aatttctttc agcagttcag tcagcagtgt
     1321 ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc ggagagtgtg gaccggtgat
     1381 agttggcccg atagaaacca tgtccatttc cggatacggt tttttgaaca gaccacattc
     1441 caggcccgcg tggataatct ggatgttcgg cgttttgttg aacagacgct gataggtttc
     1501 acgtaccaga tgcatcaccg gagaattagc gtccggctgc cagccaggat atgcgccttt
     1561 cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc agcatgctca ccacgtagtc
     1621 tttaccgctg tcgatcagag aacggatcag acagtgaatt tctacgttat tatcagtcat
     1681 ggtcaccaca ccgacgttca gggaggtttc gaccacacct tttgccacgt ctgagttgcg
     1741 gatcacaccg ttcggggtgg cgttcagcag acgaataaag gtatcgcgag atttcgcaat
     1801 cagggcagct ttatcgttcg ctacagagtc cagcaacaag gccagatttt tctctttctc
     1861 tgccagctcg tttttcagga tctcctgata ggtattcacc agagatttca ggacgtcgac
     1921 tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt
     1981 gccgccgttg aaatcgataa ggcgcagatc cagttcttct gcatgacccg ctaggaagcg
     2041 caccagcagt ttgttggcat tacccaggcc aacgtggatt tccccgccgg agtgaccccc
     2101 tttcagacct tttaaggtta acttgaaggt ttcaaaacca gctggaaccg cttcacgatc
     2161 taaatgcagg ttggaggtga agtcgatacc ccccgcacaa cccatgtaga tttcaccttc
     2221 ttcttcggag tcggtgttaa tcagaatatc agcctgcaac cagttgctct gtaagccgaa
     2281 cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc acttccagcg ggccgtgaac
     2341 cacgttttcg tcagccagaa ccgccagcgc agacgccata ccaatgccgt tatctgcacc
     2401 cagcgtggtg ccgcgcgctt taacccattc gccatcaata taaggctgga taggatcttt
     2461 cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc atatcgaggt gggcctgtaa
     2521 gacgaccgat ttacgatttt ccatacctgc ggtagcaggt ttacgaatca ggatattacc
     2581 tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc
     2641 gagttgctct tcatgatagg acgggtgagg aatagaacag attttggcaa aaatatccca
     2701 cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg tcgatcaccc
     2761 gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca agacaggctt
     2821 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     2881 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     2941 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     3001 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     3061 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctggtac
     3121 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     3181 gctacgatca cgacaaccag cgcgagctta aagtgctaaa acgcgcagaa ggcgatggcg
     3241 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
     3301 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     3361 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
     3421 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     3481 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
     3541 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
     3601 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
     3661 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     3721 cgcggcattg acccacgtga aatcctcgac gtccaggcgc gtattgtgat gagcgatgcc
     3781 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggtgg caactggatt
     3841 tatgagtggg ccatccaggc gatggtgtgg caacaaaaag cctgtgcgga agaagatccg
     3901 caactcagtg gtcgtcactg gctgcatgcg gctacgttgt acaacattgc tgcctatcct
     3961 catctgaaag gagatgacct ggccgagcaa gcgcaggctt tgtcaaaccg tgcctatgaa
     4021 gaagccgccc agcgtctgcc gggcacgatg cggcagatgg agtttaccgt acccggcggt
     4081 gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc gacagtatta
     4141 atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga acgttatttt
     4201 gcgccgcgcg gcattgcgat gctgactatt gatatgccgt cggtgggctt ttcatcaaaa
     4261 tggaagctca cccaggactc cagcctgttg catcagcacg ttttaaaggc tctgcctaac
     4321 gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt
     4381 gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg
     4441 gtagttcata ccctgttgag tgattttaag tgccagcaac aggtgccgga aatgtatctt
     4501 gacgttctgg cgagtcgttt ggggatgcat gatgcttccg acgaagcgtt acgcgtggag
     4561 ctgaatcgct attcattaaa agtgcaagga ttgctggggc gtcgctgccc aacgccaatg
     4621 ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg cttaatcacc
     4681 tcatcatctg ctgacggtaa attattagag atcccattta acccggtgta tcggaatttt
     4741 gacaaaggtc ttcaggaaat caccggctgg atcgaaaaac gcttgtgtta aaaatttgct
     4801 aaaatttgcc aatttggtaa aacagttgca tcacaacagg agattgcaat gacgttaccg
     4861 agtggacacc cgaagagcag attgatcaaa aaatttaccg cactaggccc gtatattcgt
     4921 gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg
     4981 aaaccggcac cggaagtgcg tgagttctgg ggctggtgga tggagcttga agcgcaggaa
     5041 tcccgtttta cctacagtta ccagtttggt ctgttcgata aagcaggcga ctggaagagt
     5101 gttccggtaa aagacactga agtggttgaa cgactggagc acaccctacg tgagtttcac
     5161 gagaagctgc gtgaactgct gacgacgctg aatctgaagc tggaaccggc ggatgatttt
     5221 cgtgatgaac cggtgaagtt aacggcgtga gtgaaatgtg ccggatgtat cacatccggc
     5281 aatatccatt aaaactgata ggtcatgcca accgcgacaa tatcatcatt attaatattc
     5341 aatttgttat cgctatccag ttggttgatt ttataatcaa caaacgctga catatttttg
     5401 ttgaaataat acgtagcacc gacgtcgata taattgacca gatcttcatc accgatacct
     5461 tcaatatctt tccctttcga taagacataa cccagcgatg gacgcagacc aaagtcaaac
     5521 tggtattgag cgaccgcttc aaagttctgt gtcttattgg caaagccgcc agttattggc
     5581 gtcattttgc gtgtttcaga atagaaagtt gccagataaa tattattggc atcgtatttc
     5641 agacctgttg cccatgcttc tgcacgcttg cctgtgccac ggctttgcag gttctgctcg
     5701 ttggtgcgat ctgagttggt ataggcccca ctaatggcga aatcgctgcc gccaaagtca
     5761 tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt taacgtcgcg gttttcgttt
     5821 ttcccttgat attgcagggt taagttcagg ccatcgataa cgccgaagaa gtcggtgttc
     5881 cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggaa
     5941 tcgccaccaa attccgggaa catatcggtc caggcttcca cgtcatacaa cgcgcccagg
     6001 ttacgaccat aatcgaaaga acccaaatct ttatatttca acccggcaaa agcgagacgc
     6061 gttttctgct gtgcagtatc actctccgct ttatttccgg caaactccgc ttcccaccga
     6121 ccataaccag tcagttgatc gttaatttgt gtttcacctt taaaaccaaa acggatataa
     6181 ctctggtcgc catctttact ggcgttatca ctcatataat gcatagcttt aactttgcca
     6241 tagacatcca gtttattacc gtctttatta tatatttctg cagcctgtac agatgcagat
     6301 gccacaatgc ccatcaccac taatgccaga gtgctctttt tcattttcat tcctgatttt
     6361 aattaacgcg cgaatattca gcgggagagc cccgttgaaa acaggaaagt ttttaacctg
     6421 agattgttaa agatatatta cagattaata atattcttaa aatgtggtaa tttattaaat
     6481 ctgtaataaa agcgtaaaca actgccgcta ggcttgctga tcccgcgcaa caaaacgcca
     6541 tgctttgctc gcagatggtt ggcaaccgac gacagtcctg ctaaaacgtt cgtttgatat
     6601 catttttcct aaaattgaat ggcagagaat catgagtgac agccagacgc tggtggtaaa
     6661 actcggcacc agtgtgctaa caggcggatc gcgccgtctg aaccgtgccc atattgttga
     6721 acttgttcgc cagtgcgcgc agttacatgc cgccgggcat cggattgtta ttgtgacgtc
     6781 gggcgcgatc gccgccggac gtgagcacct gggttacccg gaactgccag cgactatcgc
     6841 ctcgaaacaa ctgctggcag cggtagggca gagtcgactg attcaactgt gggaacagct
     6901 gttttcgatt tatggcattc acgtcgggca aatgctgctg acccgtgctg atatggaaga
     6961 ccgtgaacgc ttcctgaacg cccgcgacac cctgcgtgcg ttgctcgata acaatatcgt
     7021 tccggtaatc aatgagaacg atgctgtcgc tacggcagag attaaagtcg gcgataacga
     7081 taacctttct gcgctggcgg cgattctggc gggtgccgat aaactgttgc tgctgaccga
     7141 tcaaaaaggt ttgtataccg ctgacccgcg cagcaatccg caggcagaac tgattaaaga
     7201 tgtttacggc attgatgacg cactgcgcgc gattgccggt aacagcgttt caggcctcgg
     7261 aactggcggc atgagtacca aattgcaggc cgctgacgtg gcttgccgtg cgggtatcga
     7321 caccattatt gccgcgggca gcaagccggg cgttattggt gatgtgatgg aaggcatttc
     7381 cgtcggtacg ctgttccatg cccaggcgac tccgcttgaa aaccgtaaac gctggatttt
     7441 cggtgcgccg ccggcgggtg aaatcacggt agatgaaggg gcaactgccg ccattctgga
     7501 acgcggcagc tccctgttgc cgaaaggcat taaaagcgtg actggcaatt tctcgcgtgg
     7561 tgaagtcatc cgcatttgca acctcgaagg ccgcgatatc gcccacggcg tcagtcgtta
     7621 caacagcgat gcattacgcc gtattgccgg acaccactcg caagaaattg atgcaatact
     7681 gggatatgaa tacggcccgg ttgccgttca ccgtgatgac atgattaccc gttaaggagc
     7741 tggccgatgc tggaacaaat gggcattgct gcgaagcaag cctcgtataa attagcgcaa
     7801 ctctccagcc gcgaaaaaaa tcgcgtgctg gaaaaaatcg ccgatgaact ggaagcacaa
     7861 agcgaaatca tcctcaacgc taacgcccag gatgttgctg acgcgcgagc caatggcctt
     7921 agcgaagcga tgcttgaccg tctggcactg acgcccgcac ggctgaaagg cattgccgac
     7981 gatgtacgtc aggtgtgcaa cctcgccgat ccggtggggc aggtaatcga tggcggcgta
     8041 ctggacagcg gcctgcgtct tgagcgtcgt cgcgtaccgc tgggggttat tggcgtgatt
     8101 tatgaagcgc gcccgaacgt gacggttgat gtcgcttcgc tgtgcctgaa aaccggtaat
     8161 gcggtgatcc tgcgcggtgg caaagaaacg tgtcgcacta acgctgcaac ggtggcggtg
     8221 attcaggacg ccctgaaatc ctgcggctta ccggcgggtg ccgtgcaggc gattgataat
     8281 cctgaccgtg cgctggtcag tgaaatgctg cgtatggata aatacatcga catgctgatc
     8341 ccgcgtggtg gcgctggttt gcataaactg tgccgtgaac agtcgacaat cccggtgatc
     8401 acaggtggta taggcgtatg ccatatttac gttgatgaaa gtgtagagat cgctgaagca
     8461 ttaaaagtga tcgtcaacgc gaaaactcag cgtccgagca catgtaatac ggttgaaacg
     8521 ttgctggtga ataaaaacat cgccgatagc ttcctgcccg cattaagcaa acaaatggcg
     8581 gaaagcggcg tgacattaca cgcagatgca gctgcactgg cgcagttgca ggcaggccct
     8641 gcgaaggtgg ttgctgttaa agccgaagag tatgacgatg agtttctgtc attagatttg
     8701 aacgtcaaaa tcgtcagcga tcttgacgat gccatcgccc atattcgtga acacggcaca
     8761 caacactccg atgcgatcct gacccgcgat atgcgcaacg cccagcgttt tgttaacgaa
     8821 gtggattcgt ccgctgttta cgttaacgcc tctacgcgtt ttaccgacgg cggccagttt
     8881 ggtctgggtg cggaagtggc ggtaagcaca caaaaactcc acgcgcgtgg cccaatgggg
     8941 ctggaagcac tgaccactta caagtggatc ggcattggtg attacaccat tcgtgcgtaa
     9001 ataaaaccgg gtgatgcaaa agtagccatt tgattcataa ggccattgac gcatcgcccg
     9061 gttagtttta accttgtcca ccgtgattca cgttcgtgaa catgtccttt cagggccgat
     9121 atagctcagt tggtagagca gcgcattcgt aatgcgaagg tcgtaggttc gactcctatt
     9181 atcggcacca ttaagaaaat taataactta gcttaacttc tctaagaaat tgtatcttct
     9241 gttatcactt acaaacgcct atacgttgtt atgggctttg gtagtgtaac acgtatgggc
     9301 gagtagttcc caggctcaac ctcgtaacaa gttcctttga agctggaaga cgtgccaccg
     9361 tctctaaacc ttctaccaaa actcttagca agatgttcca cacctggcgc ggtgtccaat
     9421 gatgctacac gtcgtgagac gttgcagagg ataaacaggc taaatgatgg gaagagtttt
     9481 aagtgcaata gtggattaaa gcccacctct tcaaaaccta acttgatcgg gaaagaatca
     9541 agctctagca cggtttcgtc caccggagta caacgggtgc ctgataagag aagaactgct
     9601 aagacttcat agcttttgtg aaaaagccct ttttagtgga tacgcctacg gcgcttattt
     9661 tttggtataa ccatcaaagg cgctttatgg ctcctattgt cttgaaaatg tatctttact
     9721 tgtattgtgt tttatgcgta gtctccctgt gtagtatgta acttgttaat tttcatatga
     9781 atacagaggc acacgatgaa aaacattgca gcaatcaaac gtaataaccg caagattcac
     9841 gctcgtaagt tcctgtctac gccagaagga aaagcctggc tagagcgtaa acagagagag
     9901 aacgaagaaa gaaaactcct tagtgagttg aaatggctta gagaggattt ctgaaaatct
     9961 ccagaacgca acaggacgca ttctaacgct gttttcatta ctcggtatag ggataggtga
    10021 tgtgtgttta atgcgtctgt gcgcttgttt tgtgccttat tttgcatttt cataatgcgg
    10081 aagagaacac ggaaaacctt aaaattaatt attggggtat ttaaggctat tttcaggtga
    10141 tttaagggta ttaaacttga tttaattgat taaaatataa acagataatt atgtatctta
    10201 ttgatttata aggttgtttt taagtaaagg taaataaaaa ataaatagcc ataaatagct
    10261 ataaatacat cttaatacct atatcccttc ccttatggct agattgcgag aggtgcttaa
    10321 cgcgattttc agcgcccccc gatctggaaa atccagaatc cccaaatatc agcaaggaat
    10381 tttcttgcgt aatcaacatc gtttttacct attaaatcat tacacagaaa gcctttctgt
    10441 aatgtctctc actaaacttt cactaaacac gagaatgatc ggttttatgt cggatcagat
    10501 gctagaaaca gcaccacgtc tcacaagggc tgtaagcgat gaaaccagtg tttatgcggg
    10561 tgcaggtcaa aatataggcc aaaatccatt taacataata aatgttatat gtgctaagga
    10621 acacatataa cgccttgaat tgatgtatca gtggaaaagt gatggatttt ttacgtagga
    10681 gcacgaaagg gcttctattg acttttgtgt gctatgaaat ataattaaag ttttttgttt
    10741 ttattgtgtg atgtggtggg ttacacaggg gaatagatta aatggtgttc tgtttataca
    10801 ggtttatttg ttagatcaac aatttggcat ttctaaacag atgtttgaac gttgccaaat
    10861 gtcttgacaa cacaagaaaa gggcttgcta tgccaggca
//
LOCUS       DAJVQG010000133        10839 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJVQG010000133 DAJVQG010000000
VERSION     DAJVQG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10839
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.133"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(55..1338)
                     /gene="aroA"
                     /locus_tag="OQM83_003157"
     CDS             complement(55..1338)
                     /gene="aroA"
                     /locus_tag="OQM83_003157"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCS1521353.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1409..2497)
                     /gene="serC"
                     /locus_tag="OQM83_003158"
     CDS             complement(1409..2497)
                     /gene="serC"
                     /locus_tag="OQM83_003158"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCS1521354.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2696..3388)
                     /locus_tag="OQM83_003159"
     CDS             complement(2696..3388)
                     /locus_tag="OQM83_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCS1521355.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3518..5278
                     /locus_tag="OQM83_003160"
     CDS             3518..5278
                     /locus_tag="OQM83_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCS1521356.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5684..6541
                     /gene="focA"
                     /locus_tag="OQM83_003161"
     CDS             5684..6541
                     /gene="focA"
                     /locus_tag="OQM83_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCS1521357.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6596..8878
                     /gene="pflB"
                     /locus_tag="OQM83_003162"
     CDS             6596..8878
                     /gene="pflB"
                     /locus_tag="OQM83_003162"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCS1521358.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9070..9810
                     /gene="pflA"
                     /locus_tag="OQM83_003163"
     CDS             9070..9810
                     /gene="pflA"
                     /locus_tag="OQM83_003163"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCS1521359.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(9903..10079)
                     /locus_tag="OQM83_003164"
     CDS             complement(9903..10079)
                     /locus_tag="OQM83_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005043118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCS1521360.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNIISRYYLRYGIW"
     gene            10179..>10839
                     /locus_tag="OQM83_003165"
     CDS             10179..>10839
                     /locus_tag="OQM83_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1521361.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGNI
                     IL"
BASE COUNT         2790 a         2711 c         2568 g         2770 t
ORIGIN      
        1 atgtgccgac gttttgataa agaaagattt ggctatttat tgcccgttgt tcattcaggc
       61 tgcctggcta atccgcgcca actgctcgaa ataatccgga aatgttttgg ccgtacattt
      121 gggatcaaga atcgtcactg gtgtatctga caacgccacc agcgagaaac acatcgccat
      181 ccggtgatca ttgtatgtcg cgatctcggc aaagttcagt ttttccggtg gagtgatacg
      241 aatgtaatcg tgcccctctt ctacttccgc accgacttta cgcagttctg ttgccatcgc
      301 aaacaggcga tcggtctctt taacacgcca gttatagata ttgcgcagcg tggtggtgcc
      361 ttttgcaaat aacgccgccg tggcaatggt catcgccgca tcgggaatat ggttcatatc
      421 catatcaata gcgttcagtt caccacgcgt gcaggaaata taatcatcgc cccagcaaat
      481 ggtcgcgccc attttttcca gcacatcagc aaagcgaata tcaccctgca tactgttacg
      541 tccaataccg gtcactttta cagtgccgcc tttgattgct gctgctgcca gaaagtaaga
      601 agccgaagat gcatcgcctt cgaccaaata agtacccgga gactgataag actgcccgcc
      661 ttttacgaca aattgttgat agtgctgatt ttcaatttca acaccaaacg tcttcatcag
      721 attgagtgtg atgtcgatat aaggtttaga aaccagatcg cctttaatac gaatcaccgt
      781 atcttccggc gcaagaggcg cagtcattaa cagtgcggtg aggaattggc tggaaacgga
      841 gccatcaacg tcaacgttgc cgccagtaaa gccgccctgt aaacgcaacg gcggataatt
      901 ttcttgttcc aggtaagtga tcttcgcccc gcccaggcgc agcgcatcca ccagatgacc
      961 aatcgggcgt tctttcatac gcggctcacc ggtcagcaca atatcattgc tacccagaca
     1021 aagagctgcc gccagcggac gcattgccgt tccggcgtta ccgaggaaca actccagggc
     1081 accttctgcg tgtaatggac cgccgttacc gataatttcg caacgcgtac gatcggctga
     1141 aagcgtatag cttaccccta acgctgttaa tgcattcagc atatggcgca cgtcatcgct
     1201 atccagcaga ttggttaata ctgttttgcc gtgtgctaat gccgccagca ataaagcgcg
     1261 gttagaaacg ctcttggaac cgggcagatt aatagtgcca tcgacacgag cgatgggttg
     1321 taacgtcagg gattccatga actcaactct ctacaacaga aataaaaacc ccacaggctg
     1381 gctgtgggga ttaagcaaaa tttcggcatt aaccgtgacg gcgttcgaac tcaaccatga
     1441 agtctgtcag cgctttaacg ccttccagcg gcatggcgtt ataaatagaa gcgcgcattc
     1501 cgccgaccac acggtgacct ttcagtgcat gaaggccagc agcaaaagac tcttcaagga
     1561 acaatttgtc aagcgcactg tccgccaact ggaacggcac gttcatccgc gaacggttag
     1621 ctttcgccac gtcattgcgg tagaaatcgc tgttatcaat caccccatat agcagttctg
     1681 ctttttgctg attgatttta tccatttcag ctacaccgcc gttcgctttc agccatttaa
     1741 agaccagacc agatagatac caggcaaatg tcggcggcgt gttaaacatg gagccgttat
     1801 cgttgaggat ggaataatcc agaatcgacg gacacgcgat attcgctttg cccagcaaat
     1861 cttcacgaac gatgacgatt gtcaggccag ccgggccgat atttttctgc gcgccagcgt
     1921 aaatcacgcc ataacggctg acgtcaatcg gacgggaaag aatggttgaa gagaagtcgg
     1981 cggcgaccac cacatctttg ccgaagtctg gcgtttcgtc gatggcgata ccgtcgatgg
     2041 tttcattcgg gcagtaatgc atataagcag cattatcaga gagttgccat tcacgcattg
     2101 gcttaaccgc gcgcagacca tcaacagtca ctttggcgtc aaagacatta ggcgtgcagt
     2161 attttttcgc ttctttaatg gcacttgccg cccagtaacc ggcatcaaca taatctgcgg
     2221 tggttttatc accgagaata ttcagcggta ccgcagcaaa ctgaccgcga ccaccgccat
     2281 ggcagaataa taccttgtag ttggagggga cattaagaag atcgcgaaaa tccttctcgg
     2341 cttcctctgc aacctgaatg aactctttgc cacggtgact cacttccatc accgacgtac
     2401 caagaccgtt ccagtcgcgc agttcctgtt gagcctgttt aagcacctct gccggtagca
     2461 ttgccggacc agaactaaaa ttgaagattt gagccattgc ccctcaccac gttgcgttgc
     2521 tattaacccg cggtcaatcg attgcgacca caggtaatac attattcctg tggctatcgg
     2581 ttttatcatt cagtgacaca catcgcaatg agtaaaaccg ttgagggcaa aaatgtggct
     2641 tgcatcacac aaaagaatct ctcggattat tagcaaaaag ccgatgtttt tactgtcagg
     2701 tcacccgttt tgcccgactt gccttcgtcc attctggatt tgcacagcgc ggacataatt
     2761 gtttttcccc cgctttcata tgaatgattt cactacaacg aatacaggca tagtccgccg
     2821 actccggcac cactttgtaa cgctgcgtac aatcactggg taaaataagt aaacccggtt
     2881 tcaccttgtc atcttcaaag aaatagacac tgatttgccc gttggtttcg agaatcgcca
     2941 gacgtacctg ccccagctgc tccacgccac gcaatcgtag ctccataaag aactcaaatt
     3001 ccgtcatgtt ggagttattg agtttcgacc aggccagctc gccatcttca ataataacaa
     3061 ccggcttacc ttccagtaga tcttccagtt tttcactgtg cgccatcaac cacattacca
     3121 ggcggtataa caacgccaga gtgataaaga cgataagtac cggcaccatc ggcacatcat
     3181 cataaaacgc cacatctccc gccgctgaac ccagcgtgag aatgattaac acttcaaaca
     3241 gcgacatctg tcgcacaccg cgtcttccgg tcattttgag gaacaaaaaa accagtacaa
     3301 aggtataaag actacgtagt gcaacttcgc caaggaaatc aaaaggcact ttatcaaatg
     3361 ccatacggtg gagatcgaac gccttcattt ttatgctctt atcgtggttt atccgctaaa
     3421 agcatagtca acagttcatc ggcatggcgg cggtgatagc gcctttgaga taaaacccgt
     3481 attattgcgc gctttccgta cgactaaagt gattttcatg acgcaaacat ttatccccgg
     3541 caaagatgcc gctctggaag attccatcgc tcgcttccag caaaaacttt cagacctcgg
     3601 ctttcagatt gaagaggcct cctggctgaa tcctgtgcct aacgtctggt ctgtacatat
     3661 tcgcgacaaa gagtgcgcac tgtgttttac caacggtaaa ggcgcaacca aaaaagcggc
     3721 gctggcttct gcacttggtg aatatttcga gcgtctctca accaactact tttttgctga
     3781 tttctggctg ggcgaaacca tcgccaacgg tccgttcgtg cattatccca acgaaaaatg
     3841 gttcccactg accgaaaatg acgatgtgcc agaaggacta ctcgatgacc gtctgcgcgc
     3901 gttttatgat ccggagaatg aactgaccgg cagcatgctg attgacctac aatccggtaa
     3961 cgaagatcgt ggtatttgcg gcctgccgtt tacgcgtcag tccgacaatc agaccgttta
     4021 tattccgatg aatatcattg gtaacctgta cgtctccaac ggtatgtccg caggtaatac
     4081 ccgcaacgaa gcacgcgttc agggattgtc tgaagttttc gaacgctacg tgaaaaaccg
     4141 cattattgct gaaagcatca gcctgccaga gatcccggca gacgtgctgg cgcgttaccc
     4201 agcagtagtt gaagccatcg aaacgctgga agcggagggt ttcccaatct tcgcttatga
     4261 cggttcgctt ggcggccagt atccggtgat ttgcgtggta ctgttcaatc ctgctaacgg
     4321 tacctgcttt gcctctttcg gtgcgcatcc tgattttggc gtagcactgg aacgtaccgt
     4381 gaccgagctg ctgcaaggtc gtggcctgaa agatttggat gtgtttactc cgccaacctt
     4441 cgatgatgaa gaagtcgctg aacataccaa cctcgaaacg cactttatcg attccagcgg
     4501 tttaatctcc tgggacctgt tcaagcagga tgccgattat ccgtttgtgg actggaattt
     4561 ctccggcacc acggaagaag agtttgctac gctgatggct atcttcaaca aagaagataa
     4621 agaagtttat attgccgatt acgagcatct gggcgtttat gcttgccgta ttatcgtgcc
     4681 tggcatgtcc gatatttatc cggctgaaga tctgtggctc gcgaataaca gtatgggcag
     4741 ccatttacgt gaaacgattc tttcgctacc aggcagcgag tgggaaaaag aagattacct
     4801 gaacctcatc gagcaactgg atgaagaagg ttttgatgac tttacccgcg tgcgtgagct
     4861 gttgggtctg gcgaccgggt cggataacgg ttggtacacc ctgcgtatcg gtgaattaaa
     4921 agccatgctg gcgctggctg gtggcgatct ggaacaggct ctggtctgga ccgaatggac
     4981 gatggagttt aactcatcag tatttagtcc ggaacgcgcc aactattatc gctgcctgca
     5041 aacgttgtta ttactggcac aggaagaaga tcgccagccg ctgcaatatc tgaatgcgtt
     5101 tgttcgcatg tacggcgcag atgccgtaga agccgccagt gcggcaatga gcggcgaagc
     5161 ggcgttttac ggcctgcaac cggtagatag cgatctgcac gcgtttgctg cacatcagtc
     5221 gttgttgaag gcctacgaaa agctgcagcg cgccaaagca gcattctggg caaaataaaa
     5281 tcaaatagcc tacgcaatgt aggcttaatg attagtctga gttatattac ggggcgtttt
     5341 tttaatgccc cgctttacat atatttgcat taataaaata attgtaatta taaggttaaa
     5401 tatcggtaat ttgtatttaa taaatacgat cgatattgtt actttattcg cctgatgctc
     5461 ccttttaatt aactgtttta gcggaggatg cggaaaaaat tcaactcatt tgttaatttt
     5521 taaaatttat ttttatttgg ataatcaaat gtttactccg tatctgcata aaaaccatgc
     5581 gagttacggg cctataagcc aggcgagata tgatctatat caatttctca tctataatgc
     5641 tttgttagta tctcgtcgcc gacttaataa agagagagtt agtgtgaaag ctgacaaccc
     5701 ttttgatctt ttacttcctg ctgcaatggc caaagtggcc gaagaggcgg gtgtctataa
     5761 agcaacgaaa catccgctta agactttcta tctggcgatt accgccggtg ttttcatctc
     5821 aatcgcattc gtcttctata tcacagcaac cactggcaca ggcacaatgc ccttcggcat
     5881 ggcaaaactg gttggcggca tttgcttctc tctggggctg attctttgtg ttgtctgcgg
     5941 agccgatctc tttacttcca ccgtgttgat tgttgttgct aaggcgagtg ggcgcatcac
     6001 ctggggtcag ttggcgaaaa actggctaaa tgtctatttt ggcaacctgg tcggcgcact
     6061 gctgtttgta cttttaatgt ggctttccgg cgagtatatg accgcaaatg gtcaatgggg
     6121 actaaacgtc ctacaaaccg ccgaccacaa agtgcaccat acttttattg aggccgtctg
     6181 tcttggtatc ctggcaaacc tgatggtatg tctggcagta tggatgagtt attctggccg
     6241 cagcctgatg gacaaagcgt tcattatggt gctgccggtc gcgatgtttg ttgccagcgg
     6301 ttttgagcac agtatcgcaa acatgtttat gatcccgatg ggtattgtaa tccgcgactt
     6361 cgcatccccg gaattttgga ccgcagtcgg ttctgcaccg gaaaattttt ctcacctgac
     6421 cgtgatgaat ttcatcactg ataacctgat tccggttacg atcggcaaca ttatcggtgg
     6481 tggtttgttg gttgggttga catactgggt catttacctg cgtgaaaacg accaccatta
     6541 atggttgtcg aagtacgcag taaataaaaa atccacttaa gaaggtaggt gttacatgtc
     6601 cgagcttaat gaaaagttag ccacagcctg ggaaggtttt accaaaggtg actggcagaa
     6661 tgaagtaaac gtccgtgact tcattcagaa aaactacact ccgtacgagg gtgacgagtc
     6721 cttcctggct ggcgctactg aagcgaccac caccctgtgg gacaaagtaa tggaaggcgt
     6781 taaactggaa aaccgcactc acgcgccagt tgactttgac accgctgttg cttccaccat
     6841 cacctctcac gacgctggct acatcaacaa gcagcttgag aaaatcgttg gtctgcagac
     6901 tgaagctccg ctgaaacgtg ctcttatccc gttcggtggt atcaaaatga tcgaaggttc
     6961 ctgcaaagcg tacaaccgcg aactggatcc gatgatcaaa aaaatcttca ctgaataccg
     7021 taaaactcac aaccagggcg tgttcgacgt ttacactccg gacatcctgc gttgccgtaa
     7081 atctggtgtt ctgaccggtc tgccagatgc atatggccgt ggccgtatca tcggtgacta
     7141 ccgtcgcgtt gcgctgtacg gtatcgacta cctgatgaaa gacaaactgg cacagttcac
     7201 ttctctgcag gctgatctgg aaaacggcgt aaacttggaa cagactatcc gtctgcgcga
     7261 agaaatcgct gaacagcacc gcgctctggg tcagatgaaa gaaatggctg cgaaatacgg
     7321 ctacgacatc tctggtccgg ctaccaacgc tcaggaagct atccagtgga cttacttcgg
     7381 ctacctggct gctgttaagt ctcagaacgg tgctgcaatg tccttcggtc gtacctccac
     7441 cttcctggat gtgtacatcg aacgtgacct gaaagctggc aagatcaccg aacaagaagc
     7501 gcaggaaatg gttgaccacc tggtcatgaa actgcgtatg gttcgcttcc tgcgtactcc
     7561 ggaatacgat gaactgttct ctggcgaccc gatctgggca accgaatcta tcggtggtat
     7621 gggcctcgac ggtcgtaccc tggttaccaa aaacagcttc cgtttcctga acaccctgta
     7681 caccatgggt ccgtctccgg aaccgaacat gaccattctg tggtctgaaa aactgccgct
     7741 gaacttcaag aaattcgccg ctaaagtgtc catcgacacc tcttctctgc agtatgagaa
     7801 cgatgacctg atgcgtccgg acttcaacaa cgatgactac gctattgctt gctgcgtaag
     7861 cccgatgatc gttggtaaac aaatgcagtt cttcggtgcg cgtgcaaacc tggcgaaaac
     7921 catgctgtac gcaatcaacg gcggcgttga cgaaaaactg aaaatgcagg ttggtccgaa
     7981 gtctgaaccg atcaaaggcg atgtcctgaa ctatgatgaa gtgatggagc gcatggatca
     8041 cttcatggac tggctggcta aacagtacat cactgcacta aacatcatcc actacatgca
     8101 cgacaagtac agctacgaag cctctctgat ggcgctgcac gaccgtgacg ttatccgcac
     8161 catggcgtgt ggtatcgctg gtctgtccgt tgctgctgac tccctgtctg caatcaaata
     8221 tgcgaaagtt aaaccgattc gtgacgaaga cggtctggct atcgacttcg aaatcgaagg
     8281 cgaatacccg cagtttggta acaacgatcc gcgtgtagat gacctggctg ttgacctggt
     8341 agaacgtttc atgaagaaaa ttcagaaact gcacacctac cgtgacgcta tcccgactca
     8401 gtctgttctg accatcactt ctaacgttgt gtatggtaag aaaacgggta acaccccaga
     8461 cggtcgtcgt gctggcgcgc cgttcggacc gggtgctaac ccgatgcacg gtcgtgacca
     8521 gaaaggtgca gtagcctctc tgacttccgt tgctaaactg ccgtttgctt acgctaaaga
     8581 tggtatctcc tacaccttct ctatcgttcc gaacgcactg ggtaaagacg acgaagttcg
     8641 taagaccaac ctggctggtc tgatggatgg ttacttccac cacgaagcat ccatcgaagg
     8701 tggtcagcac ctgaacgtta acgtgatgaa ccgtgaaatg ctgctcgacg cgatggaaaa
     8761 cccggaaaaa tatccgcagc tgaccatccg tgtatctggc tacgcagtac gtttcaactc
     8821 gctgactaaa gaacagcagc aggacgttat tactcgtacc ttcactcaat ctatgtaatt
     8881 agatttgact gaaatcgtac agtaaaaagc gtacaataaa ggctccacga aagtggggcc
     8941 ttttttagca cgagagcctt ttttgtcagc tatctatact ctaaggtgac tgccaaaaca
     9001 gactcgacgt agccttcgag ctgcgcacca acacggcctc agatgggcca catctggaga
     9061 aacaccgcaa tgtcagttat tggtcgcatt cactcctttg aatcctgtgg aaccgtagac
     9121 ggcccgggta ttcgctttat cacctttttc cagggctgcc tgatgcgctg cctgtattgt
     9181 cataaccgcg acacctggga tacgcatggc ggtaaagaag ttaccgttga agatttaatg
     9241 aaagaggtgg tgacctatcg ccactttatg aacgcttccg gcggcggcgt taccgcatcc
     9301 ggcggtgagg caatcctaca agctgagttt gttcgtgact ggttccgcgc ctgcaaaaaa
     9361 gaaggcattc atacctgtct ggacaccaac ggttttgttc gtcgttacga tccggtgatt
     9421 gatgaactgc tggaagtaac cgacctggta atgctcgatc tcaaacagat gaacgacgag
     9481 atccaccaaa atctggttgg agtttccaat caccgcacgc tggagttcgc caaatatctg
     9541 gcgaacaaaa atgtgaaggt gtggatccgc tacgttgttg tcccaggctg gtctgacgat
     9601 gacgattcag cgcatcgcct cggtgaattt acccgtgata tgggcaacgt tgagaaaatc
     9661 gagcttctcc cctaccacga gctgggcaaa cacaaatggg tggcaatggg tgaagagtac
     9721 aaactcgacg gtgttaaacc accgaagaaa gagaccatgg aacgcgtgaa aggcattctt
     9781 gagcagtacg gtcataaggt aatgttctaa tttccccgta aagcggcaac tttattgagt
     9841 tgccgctttt ttattccgca cttcattctg ctacagcgtt tttaacacat cttatagtgc
     9901 atctaccata tcccgtacct gagataataa cgactgataa tattacgccg atataaatcg
     9961 agttccgtca cctgtatttt ccgctccatt gcccgctgat ggatcgcatc agcaatatgt
    10021 gcggtcaatg aatcatgacg aggatgggcc cataccaaat aaatacgttc agactgcata
    10081 attttacctc cacgttaagt tctggagcgc actttacgca ggcggcataa taatgtaaac
    10141 gtgatgattc aggatgagtt aatgaaggat tttcactaat gcggatgaat atgtctgact
    10201 ttgccacttt ctttgctgtg gcccgtaatc aaagctttcg tgcagcgggc gatgagttag
    10261 gcttatcctc gtccgccatt agccatagta ttaaaacact ggaacaacgt cttaaaattc
    10321 gcttattcaa tcggacaacc cgaagcgtat cgttaacgga agcgggttct aatctctatg
    10381 aacgattgcg cccagctttc gacgaaattc agatcatgtt agatgagatg aatgattttc
    10441 gtctgacacc aacaggaacg ctcaaaatca atgccgcccg ggtagctgca cgtatatttt
    10501 taatgccttt actggttggt ttcacacgtg aatatcctga tattaaagtc gagctcacaa
    10561 ctgacgactc tcttgtcgat atcgtccaac agggctttga cgctggcgtt cgacttagtg
    10621 gcatcgtcga aaaagatatg atcagcgtgg cgatcggccc accagtgaaa ttatgtgtag
    10681 cagcaacgcc agagtacttt gcaagatatg gcaaaccccg ccatcctcat gatctgctta
    10741 atcatcaatg tgttgtattt cgttatccca gcggcaaacc tttccactgg caatttgcaa
    10801 aggagcagga aattgctgtc gcagggaata tcattctgg
//
LOCUS       DAJVQG010000134        10718 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJVQG010000134 DAJVQG010000000
VERSION     DAJVQG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10718
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.134"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            1..1093
                     /gene="dacC"
                     /locus_tag="OQM83_003166"
                     /pseudo
     CDS             1..1093
                     /gene="dacC"
                     /locus_tag="OQM83_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            complement(1140..1898)
                     /gene="deoR"
                     /locus_tag="OQM83_003167"
     CDS             complement(1140..1898)
                     /gene="deoR"
                     /locus_tag="OQM83_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCS1521362.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1956..2552)
                     /gene="ybjG"
                     /locus_tag="OQM83_003168"
     CDS             complement(1956..2552)
                     /gene="ybjG"
                     /locus_tag="OQM83_003168"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCS1521363.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            2837..4064
                     /gene="mdfA"
                     /locus_tag="OQM83_003169"
                     /pseudo
     CDS             2837..4064
                     /gene="mdfA"
                     /locus_tag="OQM83_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(4105..4389)
                     /locus_tag="OQM83_003170"
     CDS             complement(4105..4389)
                     /locus_tag="OQM83_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521364.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(4475..5290)
                     /gene="ybjI"
                     /locus_tag="OQM83_003171"
     CDS             complement(4475..5290)
                     /gene="ybjI"
                     /locus_tag="OQM83_003171"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCS1521365.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(5290..6498)
                     /locus_tag="OQM83_003172"
     CDS             complement(5290..6498)
                     /locus_tag="OQM83_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1521366.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            6582..7118
                     /gene="rcdA"
                     /locus_tag="OQM83_003173"
     CDS             6582..7118
                     /gene="rcdA"
                     /locus_tag="OQM83_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCS1521367.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(7293..8978)
                     /locus_tag="OQM83_003174"
     CDS             complement(7293..8978)
                     /locus_tag="OQM83_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCS1521368.1"
                     /translation="MNINVAELLNGNYILLLFVVLTLGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            9248..9625
                     /locus_tag="OQM83_003175"
     CDS             9248..9625
                     /locus_tag="OQM83_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCS1521369.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(9655..9912)
                     /gene="grxA"
                     /locus_tag="OQM83_003176"
     CDS             complement(9655..9912)
                     /gene="grxA"
                     /locus_tag="OQM83_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCS1521370.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            10072..10359
                     /locus_tag="OQM83_003177"
     CDS             10072..10359
                     /locus_tag="OQM83_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCS1521371.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            10343..>10718
                     /gene="nfsA"
                     /locus_tag="OQM83_003178"
     CDS             10343..>10718
                     /gene="nfsA"
                     /locus_tag="OQM83_003178"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCS1521372.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLG"
BASE COUNT         2765 a         2650 c         2715 g         2588 t
ORIGIN      
        1 gatgcgcgtg catggatttt aatggattac gccagcggta aagtgctggc agaaggcaac
       61 gcggatgaga aactggatcc cgcgagcctg actaaaatca tgaccagcta tgtggttggg
      121 caggcgctta aggccgataa gattaaactc accgatatgg tgacggtcgg taaagatgcc
      181 tgggcgacgg gaaatccggc actgcgtggt tcatcggtaa tgttcctcaa accgggcgat
      241 caggtttcgg tggcagactt gaacaaaggt gtgattatcc agtccggtaa tgacgcctgt
      301 attgcgctgg ccgattacgt tgccgggagc caggagtcat ttattggttt gatgaatggt
      361 tatgccaaaa aaactgggtc tgaccaacac taccttccag acggtgcacg gtctggatgc
      421 gccggggcag ttcagtaccg cgcgcgatat ggcattgctg ggtaaagcat tgatccacga
      481 tgtgccggaa gagtacgcca ttcataaaga gaaagagttc accttcaaca aaattcgtca
      541 gcctaaccgt aaccgtctgc tgtggagcag caatctgaat gttgatggca tgaagacagg
      601 aaccacggca ggtgcgggat ataatctggt tgcttcggct acccagggtg atatgcgttt
      661 aatctccgta gtgctggggg cgaaaactga ccgtatccgt tttaatgagt ctgagaaatt
      721 attgacctgg ggtttccgct tctttgaaac tgtgacgcca attaaacctg atgccacctt
      781 tgtgactcag cgcgtctggt ttggtgataa gagcgaagtg aatctcgggg caggcgaagc
      841 gggctccgtg accataccgc gtgggcagct gaaaaacctg aaggcgagct acacgttaac
      901 ggaaccgcag cttaccgcac cgctgaaaaa aggtcaggtt gtcgggacca ttgatttcca
      961 gcttaacggt aaatccattg agcagcgtcc gctgatcgtg atggaaaatg tggaagaggg
     1021 cggattcttt ggtcggatgt gggatttcgt gatgatgaaa ttccatcagt ggttcggcag
     1081 ctggttctct taatcttctg ataaccggat ggcgcgaaac gtcatccggt tatacgtcat
     1141 taatacatca acttaatacg gtgcgtctgc gcgtacttca catactcatc ttccgggcaa
     1201 caatcactca ccacaatatc aaagcgtttc aggtcaccca tgcgcgccgg acgcaccttg
     1261 ccaaatttac tgtggtcgac aaccagcaca tgcttttgcg ccatcgacat ggcccagtgt
     1321 tttaccggca actcttcaag attaaaacag gtagcgcctt tactgacatg cacgcccgcc
     1381 gcggaataaa aagcgatatc cgggcaaaaa ttattcaacg tttgctgaaa atcgatgggg
     1441 ttgaaaatgg cgttgctggc gtgaaattca ccaccgcaca gaaatgcgcg gcaatggggt
     1501 ttctctttca gcgccagaaa ggtatttagc gaataacaaa cggcggtaaa agggatttca
     1561 ttatcaatcg cttcaataat ccacggcgtg gtggtgccac agtcaaaaaa gagggtctga
     1621 tcgggttcta ccagtgtcgc agccagtttt gccgcccggc gtttttcttc caccaggcgg
     1681 gatttttgat cgcttaacag gtaatggctg gcactgcgcg gttccagaac aatatagccg
     1741 ccgagcaaaa cgacgggcgc actgtggttg ttcagatcgc gacgaatcgt catctccgaa
     1801 accccaagca gggcggcggc gtctttaaga tgtaacttat cgctgcgttt taattcttgc
     1861 agcagctgcc cgatacgctc ttcgcgacgt gtttccataa tccctctgaa tagttattga
     1921 agcgagccgc tcaatactac actttttagc agagatcagt cacgcaccca gcctttgcgg
     1981 atcggtaatg caaaacagac gcgataccac gattgcagac gttgatagag tttatgcccc
     2041 atcgcttgcc agataatctg ggcactaagg cagccaatca tacctgccag caatccaccg
     2101 agcatatcca gcggccagtg gacgccaaga taaacgcgcg accaggcaat gacgacggcc
     2161 agaaccatta aaagtgagcc ggaccacagg cgatgccagc ataaaaatgc cagtgcaaag
     2221 gtgaaaatca ccgtaccgtg atcgctcggg aatgagtcat ccgccgcatg atgcaggaag
     2281 ttatagccga tattttcgac aaagggtcgg tcgtgcggaa aaagatgtcc catcgtccag
     2341 gacacaaaca ggctgaccgc cagcgcgata gcgattttta tcaccagttg ccgttgtgct
     2401 gtaagccccc acaaccaaag taccgcggcc agcaacggca ccacggtaat caaatcttta
     2461 gcaataaaaa tcgccaacga gatcatccac ggagccgagt ctggcgtcgc gttaataaga
     2521 gagaatagag agagattcaa attttccagc ataacttccc gacgcaaagt gattaaaagg
     2581 gagccaatac aggcaagtcg ttgagaataa agtgcaggtt aaactgggta aagcggcatc
     2641 gtcttatttc cctcaagcgg cctgtttacg gtgggtgatt gtaacgggca taggttaaat
     2701 aaaacttaaa gaaagcgtag ctatactcgc aataatgtaa gaatgtgctt aaccgtggtt
     2761 tcagctacaa aattcgcttt ctcgttagct gcgcttttat taaactctgc gcgattatta
     2821 ttggcgaaga aattgcatgc aaaataaatt agctttcggt gccaggcttg gacgtcaggc
     2881 gttacttttc cctctctgtc tggtgcttta cgaattttca acctatatcg gcaacgatat
     2941 gatccaaccc ggtatgttgg ccgtggtgga acaatatcag gcgggcattg attgggttcc
     3001 tacttcgatg accgcgtatc tggcgggcgg gatgttttta caatggctcc tggggccgct
     3061 gtcggatcgt attggtcgcc gtccggtgat gctggcgggt gtggtgtggt ttatcgtcac
     3121 ctgtctggca atattactgg cgcaaaacat tgaacaattc accctgttgc gcttcttgca
     3181 gggcataagc ctctgtttca ttggcgctgt gggatacgcc gcaattcagg aatccttcga
     3241 agaggcggtt tgtatcaaga tcaccgcgct gatggcgaac gtggcgctga ttgctccgct
     3301 acttggtccg ctggtgggcg cggcgtggat ccatgtgctg ccctgggagg ggatgtttgt
     3361 tttgtttgcc gcattggcag cgatctcctt tttcggtctg caacgagcta tgcctgaaac
     3421 cgccacgcgt ataggcgaga aactgtcact gaaagaactc ggtcgtgact ataagctggt
     3481 gctgaagaac ggccgctttg tggcgggggc gctggcgctg ggattcgtta gcctgccatt
     3541 gctggcgtgg atcgcccagt cgccgattat catcattacc ggggagcagt tgagcagcta
     3601 tgaatatggc ttgctgcaag tgcctatttt cggggcgtta attgcgggta acttgctgtt
     3661 agcgcgtctg acctcgcgcc gcaccgtacg tgataattat gggcggctgg ccgattatga
     3721 ttggtctgtt ggtcgctgct gcggcaacgg ttatctcatc gcacgcgtat ttatggatga
     3781 ctgccgggtt aagtatttat gctttcggta ttggtctggc gaatgcggga ctggtgcgat
     3841 taaccctgtt tgccagcgat atgagtaaag gtacggtttc tgcggcgatg ggaatgctgc
     3901 aaatgctgat ctttaccgtt ggtattgaaa tcagcaaaca tgcctggctg aacgggggca
     3961 acggacagtt taatctcttc aaccttgtca acggaatttt gtggctgtcg ctgatggtta
     4021 tctttttaaa agataaacag atgggaaatt ctcacgaagg gtaaaaaaat gcctgactgc
     4081 tttgtgcgat caggcattct cgaattaatg gtgatggtcg tcaatctggt gttcgataac
     4141 catcccttca cctacgctgg caagatggcg agcataagga tgcgggcggt aagccggagc
     4201 tggcgcagga gccacataga cggtttgcgg cacagtcgcg acgctgaccg cttgtggaac
     4261 gctgactgaa tcaggaacta caaccacttt gtatccactc ggcacatcga cggtgacact
     4321 ttgcgccatc gccacaccag taaagcccat taaaagcgcg cccaacagta gacaattttt
     4381 cataatcatt tcgcctgaaa taatgcagcg ggttaatttc cgcgaattat gcagccttca
     4441 ttcaggtatt gtgttgccgg gcgtgctgcc gtttttattg gtcaaatggc gcttcgtgtt
     4501 taagcacttt atcgatcaca tccagtacgc cttcacggtt attggagcct gcccggtatt
     4561 ttgccgctgc gacgaccgcg ctgccggcat tttccattgc aaaactaaag cctgcctgac
     4621 gcagcatctc aatatcgtta ccgccatcgc caaagaccac cacttcgctg tcgtctattc
     4681 cccataattt ctgcagttgg cgaaggccat tggctttatg tacgccgggg ataatcagat
     4741 cgatgctgcc gttgccggtg tggactggca ccataatatc gccgatggcc tcatgtaatg
     4801 ctttttgtac ttgtggaatc agttcatcgg aaagattcag gccaaactta aagaaaatat
     4861 cttctaagtt gtcaaagtta tcgacgtatt ccagacggtg ataatacatt tctgccaccg
     4921 ttttcatggc atcgtcatat tttttgagcg tataggcgct attttttccg caggcaataa
     4981 tttccacttc cgggcgcgtc agcaaatgtt ccacgacagt agtaaaggca tccttcgaca
     5041 gctcgccatt aaaaacatct ttgccttcac tcactaccca gccgccgttt tcagccacaa
     5101 aggcaatttc attagcaatt tcagggaaga aagagagcaa ctgataatat tgattcccgc
     5161 tggcgaccac aaagcgaatg ccttgtgctt tcatttgctg atactgagcc ataaaccgct
     5221 cacggttata ggttttttga tcgcttaaga aagtaccatc catgtctacc gcaattaatt
     5281 taatgctcat caactattct ccatcgccgt ccgcgttttg gtatcgggtt tggcgacggc
     5341 tttcgcgaca atagccgcga gaataaccag cgccagtaca accagcattg cactacgtaa
     5401 tccataatgt tcgccgagat agcccagcag cggcggcccg acgaggaaag ccagataacc
     5461 ggtcgttgct accacactga cgcgggttgg tgcatcgggg ccggtatcgc tggcggcaga
     5521 aatggtcagc gggaagccca gcgaggcacc cagtccccag agtacaacag acaccccagc
     5581 gacccaggcg ctatcgacaa aaataatcag cccaataccc aacgccccca ttagtgcact
     5641 ggcccgaacc acggcaacgc gactgtaacg gtcgatgaac caaccgccag tgaagcgtcc
     5701 aacggtcatc cctagggtaa aaccggcata aatcagcgag ccggaagtag ggctaaaacc
     5761 gtgaccgtca accattaata agggtaacca gtcgttggca gaaccttcgg caaaggccat
     5821 cgccagcact acaacaccaa tcaacagcaa ctggatatcg cgataaaaag gaatgccttt
     5881 ttcgccatgc tgggtgccat cggcagcatt tttgcccgta ccgtcaggga ttgcctgaat
     5941 ggcgatataa ataggcgcga tacctaccag cgccgccaat aaaatgtgca ccgttgccgg
     6001 aacgccaaag gccgtcagtg ccatcccgac accagcgcct gccagcgtgc ctaggctata
     6061 aaaaccgtgc atcatcggca aaaccgtttt attcatttct cgctcaacgg cggcaccttc
     6121 aacgtttatc gccacttccg cagaaccaaa acttgcccca aagacgccga gaccaacggc
     6181 aaagagcagg ggcgatgtca gccagagtgc cagacttaat atcatcatcc cgatcaatgc
     6241 gcaggacatc gtgaccagga tgacattacg tgtcccaaag cgtttcacta accacgccga
     6301 gcagagaata ccgctcatcg aaccgatcga cagaccaaag agaacaccgc ccatttcagc
     6361 gatcgagaca gagagaatat cgcggatagc aggcgtacgg gttgcccagg acgccattaa
     6421 caggcctggc aaaaagaaga acataaacag cgcccaggtt cggcgtttca atgcattacg
     6481 tgaagaattt acggtcatag atcacgtcaa aataagaaga gggaagacaa cattagcaag
     6541 gttgtgtaca tttgtacaca attgcagaca gaggaaatga catgcgtcgc gctaacgatc
     6601 cgcaacggcg agaaaaaatt atccaggcca cgctggaggc ggtgaaactt tacggaatac
     6661 atgctgttac gcaccgcaaa attgctaccc ttgccggggt accgttggga tcgatgacct
     6721 actatttttc aggaattgat gagttgttgc tggaggcgtt cagcagtttt actgagatta
     6781 tgtcccggca atatcaggca ttttttagcg atgttagtga tgctcaaggc gcatgccagg
     6841 ctatcaccga tatgatctac agctcacagg ttgcaacgcc ggataatatg gagctgatgt
     6901 accagctcta cgcgctggct agccgaaaac cgctattaaa aacggtaatg caaaactgga
     6961 tgcagcgcag tcagcaaacg ctcgaacaat ggtttgaacc cggaaccgcc cgcgcgcttg
     7021 atgcgtttat tgaggggatg acgctgcatt ttgtcaccga ccgtaagccg ctatcgcgcg
     7081 aggagatttt gaggatggtt gagagggttg cagggtagta gataaatttt agataacaaa
     7141 aaacccatca accttgaacc gaaatggcgg ggttgatggg ctccacaaaa tggggacatc
     7201 aaagaaaagc agtggcacta attaagactg atgccctgcg gaaaagttct gcggttgtgc
     7261 aaaaaaattt cattttcagg gcaacttcag ttttatccta atcctggcca taccatgacg
     7321 atgattgtcc ctgccagcgt cagcaggacg ttggcgattg cgtaggtgcc cgcatagccc
     7381 agcgcaggga tgttactgcg agctgtatca ctgatgatct ccattgccgg cgcgcaggtg
     7441 cgtgcgccca tcattgcgcc gaacaacagt gcgcggttca ttcgcaatac ataagcaccg
     7501 aacaagaaac agataaccac gggcacaaga ctgacaatta atccggcaat caacatctga
     7561 ccgccaatcg cgcccaggcc gttattaata ccgctaccgg cgctcagacc aacgcctgcc
     7621 ataaacacca tcaagccgaa ctctttcacc atgcttaatg caccttgcgg aatgtaaccg
     7681 aaggtcgggt ggttagcacg cataaagccc agcataattc cggcgaataa caacccggca
     7741 gcgttcccca tgccgaaact gaatgtgctg aactggaagg tgatcatccc gatcatcagc
     7801 ccaataacaa agaaggcgca aaatgccagc aggtcagtga cctggctgtg aatcgagata
     7861 aagccgatgc gatcggcgat ggtttttacg cgacgggcat caccgctgac ttgtaaaacg
     7921 tcacctttgt taagcacgac gttgtcatct atcggcatct caatctggct acgaatgacg
     7981 cggttaagga agcaaccgtg atcggtcaac ttcagttgtg cgagacgttt acctacagcg
     8041 ttatggtttt taacgaccac ttcttcagtg acgatacgca tgtcgagaag gtcacgatcg
     8101 aaaacttctt taccgttacg gaagctggga tcgagtcggg catgggcgtc gggatagcct
     8161 accaacgcta tttcatcgcc catttgtagc acggcatcac cgtctggatt tgccagaatc
     8221 ccgttacgtc gaatacgttc aatgtagcag ccggtttgtc gataaatacc cagttcacgc
     8281 agatttttgc cgtcggtcca ggccaccagc tccgggccga cgcgataggc gcggatcacc
     8341 ggtaaataaa ccttacggtt ggcatcagtg tccaggccac gttcgcgggc gatttgctgg
     8401 gcgctggtct gtaagtcctg atgctgcaat ttcggcaagt aacgcgcacc aacaatcaaa
     8461 ctcaccagac cgattaaata ggttaaggca tacccgaggc tcagattatc cagtgccagt
     8521 gagagctgcc tgctttccat gccggaatga cgcagtgtat cgccagcacc gaccagaacc
     8581 ggtgtcgacg tcatagagcc tgctaacata ccggccgtca ggccaatatc ccagccaaac
     8641 agcttaccta accctaaggc gatcaccagc gcactgccaa ccatcaccag tgctaacatt
     8701 aggtaatttt tcccatcgcg aaaaaaaatg gaaaaaaagt tcggtccggc ttcgaccccg
     8761 acgcagaaaa taaacagcat aaagccaaga ttaagcgcat cggtgttaat gctgaaatgt
     8821 tgttggccta ataacagcga tacgactaaa acgccaatgg aattacccag ttggatcgaa
     8881 ccaagtcgta actttccgag acatagccca agcgtgagga ccacaaataa taacagaatg
     8941 taattcccat ttaacaattc ggcgacgttt atattcacgg aggctaactt cttgtttact
     9001 agtaagctgt tgaaagaaat ggtaatttac gataatgttt tttaccagaa ttcagggcgc
     9061 agattcattc agcgcaccta aacgatagta aagtaacaat atattttact agtgtaatca
     9121 cattaggtat caacggctat atgaattgcg ttggcctata ttagcatgga atgcgaagcg
     9181 gctttatctt actgaacgcc acactggcga aaaatgtgtt cgatagacgc agtgtcagga
     9241 ggaacgagtg aaacataaac aacgttgggc gggggcaatc tgctgttttg tcctcttcat
     9301 tgtggtgtgc ctttttctgg cgacgcacat gaaaggcgct tttcgggctg ccgggcatcc
     9361 tgaaatcggc ttgctgtttt tcattcttcc tggagcagtt gccagtttct tttcacagcg
     9421 tagagaagtc ctgaaacctc tatttggcgc aatgctggcg gcaccctgtt caatgctcat
     9481 tatgcggctg tttttttcac cgacgcgctc attctggcaa gagctggcat ggttactaag
     9541 cgcggtgttc tggtgtgcgc tgggggcact gtgtttctta tttatcagta gtttgtttaa
     9601 accacagcac agaaaaaatc agtaaagccc tcaacgcgag ggcttgtcag acgatcaggc
     9661 gtccagattt tctttcaccc atgcagcaaa atcggtatag ccgccgatat gttgctgatc
     9721 gacaaaaatc tgcggcacgg tttctacggg tttacctgcc ttttgttgta gatcttcttt
     9781 agtgatccct tccgcacgaa tatctacata ctgatactga aaatcatcgc gttcattgct
     9841 caatttctca gccagatctt ttgcacgcac acagtaaggg caacccgaac gaccaaaaat
     9901 aacggtttgc attatttctc tcctcataga tttatgcctg taatgatcac gctaaaatgt
     9961 attcgctgaa agtaggttta acctgttgca ttaattgcta aaagctataa ctgttaaaca
    10021 caatacagtg aaaagtttta gactgaaggc tcactttgca gagggaagcg tatgcgcgcg
    10081 atcggtaaat tgcctaaagg cgtgttgata ctggaattta tcggaatgat gctactggcg
    10141 gtggcgctgc tgtcggtaag cgactccctg tcgctgcctg agccattttc tcggccagaa
    10201 gtgcagattc tgatgatttt tctcggtgtt ttgctcatgc ttcccgctgc ggtggtggtt
    10261 attcttcagg tggcaaaacg tcttgcccca cagctgatga accgtccacc gcaatattca
    10321 cgttcagaaa gagaaaaaga taatgacgcc aaccattgaa cttatttgtg gccatcgctc
    10381 cattcgccat ttcactgatg aacccatttc cgaagcgcag tgtgaggcga ttattaacag
    10441 cgcccgtgcg acgtccagtt ccagtttttt gcagtgtagt agcattattc gcattaccga
    10501 caaagcatta cgtgaagaac tggtgacgct gaccggcggg caaaaacacg tagcgcaagc
    10561 ggcggagttc tgggtgttct gtgccgactt taaccgccat ttacagatct gtccggatgc
    10621 tcagctcggc ctggcggaac aactgttgct cggtgtcgtt gatacggcaa tgatggcgca
    10681 gaatgcatta accgcagcgg aatcgctggg attgggcg
//
LOCUS       DAJVQG010000135        10648 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJVQG010000135 DAJVQG010000000
VERSION     DAJVQG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10648
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.135"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(136..1164)
                     /gene="malI"
                     /locus_tag="OQM83_003179"
     CDS             complement(136..1164)
                     /gene="malI"
                     /locus_tag="OQM83_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCS1521373.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1339..2931
                     /gene="malX"
                     /locus_tag="OQM83_003180"
     CDS             1339..2931
                     /gene="malX"
                     /locus_tag="OQM83_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCS1521374.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2941..4113
                     /gene="malY"
                     /locus_tag="OQM83_003181"
     CDS             2941..4113
                     /gene="malY"
                     /locus_tag="OQM83_003181"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCS1521375.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4217..5218
                     /gene="add"
                     /locus_tag="OQM83_003182"
     CDS             4217..5218
                     /gene="add"
                     /locus_tag="OQM83_003182"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCS1521376.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5254..6294)
                     /locus_tag="OQM83_003183"
     CDS             complement(5254..6294)
                     /locus_tag="OQM83_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1521377.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSCVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6538..6663
                     /gene="blr"
                     /locus_tag="OQM83_003184"
     CDS             6538..6663
                     /gene="blr"
                     /locus_tag="OQM83_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCS1521378.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6928..7143
                     /gene="ydgT"
                     /locus_tag="OQM83_003185"
     CDS             6928..7143
                     /gene="ydgT"
                     /locus_tag="OQM83_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCS1521379.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7229..7669
                     /locus_tag="OQM83_003186"
     CDS             7229..7669
                     /locus_tag="OQM83_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCS1521380.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7746..8327
                     /gene="rsxA"
                     /locus_tag="OQM83_003187"
     CDS             7746..8327
                     /gene="rsxA"
                     /locus_tag="OQM83_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCS1521381.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8327..8905
                     /gene="rsxB"
                     /locus_tag="OQM83_003188"
     CDS             8327..8905
                     /gene="rsxB"
                     /locus_tag="OQM83_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCS1521382.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8898..>10648
                     /gene="rsxC"
                     /locus_tag="OQM83_003189"
     CDS             8898..>10648
                     /gene="rsxC"
                     /locus_tag="OQM83_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCS1521383.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQV"
BASE COUNT         2450 a         2751 c         2720 g         2727 t
ORIGIN      
        1 tctcaagttg tcagaattaa acacataaac ctcctgtgaa cttcagtaaa aataaaaggc
       61 accgcctgtg tgccacattt tttatcatta accttgagga aaggacacta ccaggtatag
      121 cagataaaga gaggattatt tcgctgcaat gagccgggcg ggaataataa gattgcgtga
      181 atgcgtctct tcatgggtga ttttttgcat catgcgatcc gcaagtgtgg taccaagttc
      241 ccgcgcaggc gtgctggccc aggtaacggg tatatcatca agtgtggttg gtgtcgcatc
      301 ggtaaatgcc gccagcgaaa cttgttgctc aaagtaacga tcgactccgc tttccccgct
      361 ttgccgccct gctttcagca aaccaaacca tgcccccatc gcaatagttt cgttatagca
      421 aaccacagca ctgatggtcg ggttatgacg taaaagcgcc gtgatagctt ccgcggcttg
      481 cttctggctg gatgtgcact ccagcaccca atcgctgtga aacggcaggc caaattttag
      541 tagagttgca caatagcctc ccacccgttc tgcccgggtt aatgaggaac tttgccctcc
      601 cagccaggcg atccgctgat gcccattgcg aatgagatgc tccgtcaaca actgtgcagc
      661 ctgcatgttg tccgggcgaa ccgtatcaac atcatcaaga taactggcac gggaagcgaa
      721 aatcaccggg atagcttttt cttctgccat ccgtcgcagg tcatcgctgc ttcctgcagc
      781 cccggcaatt accacaccat cgacaccctg attcagtaac agtgaaaacc gctgtgccag
      841 ctgctcgccg tctttaccgc cgtgaagcaa aaaaaccatc cgtccctgcg cttccagagc
      901 ttccgtcaat ccggccgtca attcggcgta aaacggcgca gataaatcac ggacgatcaa
      961 accaatgacg ccgctttgcc cgccgcgcag cgccgacgcc tggcgattgc gcacaaatcc
     1021 cagctcttca atggcggcgt taacgcgttc tcctgtggcg gtagagattc gccctttgcc
     1081 actaagcacc agcgaaacgg tacttaccga cacgcccgca gccagcgcaa catcatgaat
     1141 ggttattttt ttggcggtag ccatgcagat gacctactcc ctgattatgt gacagataaa
     1201 acgttttacc ttttatttat cttatacccg ctattatcgt tgcgtaatgt gatttatgct
     1261 tcactaaaat ttgataaaac gttttatctt ctcgcgcaat ttactgaatc cagattgttc
     1321 tctacgagga gtcgttttat gacggcgaaa acagcaccga aagtcacgct gtgggagttc
     1381 ttccagcagt taggcaaaac cttcatgtta cccgtggcat tattgtcgtt ctgcggcatt
     1441 atgctcggca ttggtagttc tcttagtagc cacgatgtca tcaccctgat cccggtcctg
     1501 ggcaaccccg tgttgcaggc tatctttacc tggatgagta agattggctc gtttgctttt
     1561 agtttcctgc cagtcatgtt ctgtatcgcc atcccgctgg gtctggcacg cgaaaacaaa
     1621 ggcgtagcgg cattcgctgg cttcgtcggt tatgcggtaa tgaacctcgc ggtaaacttc
     1681 tggttgacca ataaaggcat tctgccaacc acggacgccg cggttctgaa agccaataac
     1741 atccagagca ttcttgggat ccagtcgatc gacaccggga tcctcggtgc ggtgatcgcc
     1801 ggtattatcg tctggatgct gcatgagcga ttccataata tccgcctgcc ggatgcgctt
     1861 gcattcttcg gcggtacgcg cttcgtacca atcatctcct cgctggtgat gggccttgtc
     1921 ggcctggtga ttccattagt ctggccgatt ttcgcgatgg gtattagcgg cttgggccat
     1981 atgataaaca gcgcgggtga tttcggaccg atgctgtttg gtaccggtga acgtctgctg
     2041 ttgccgtttg gtctgcatca cattctggtg gcattaattc gctttaccga cgcaggcggc
     2101 acgcaggaag tctgcggtca aaccgtcagc ggcgcattga ccatcttcca ggcgcaattg
     2161 agttgcccga ccactcacgg tttttctgaa agcgccacgc gtttcctttc gcaaggcaaa
     2221 atgcctgcgt ttctcggcgg tctgccaggt gcagcgttag cgatgtatca ctgcgcgcgc
     2281 ccggaaaatc gccataaaat taaagggctg ctgatttctg gcctgatcgc ctgtgtcgtt
     2341 ggcggcacga ccgaaccgct ggaattcctg ttcctgttcg tggcgccagt tctgtatgtc
     2401 atccacgcgc tgttaaccgg cctcggcttc actgtcatgt ctgtgctcgg cgtcaccatc
     2461 ggtaataccg acggcaatat catcgacttc gtggtgttcg gtattttgca tggtctgtca
     2521 accaagtggt acatggtgcc agtggtggcg gcaatctggt ttgtcgttta ctacgtcatc
     2581 ttccgtttcg ctatcacccg cttcaatctg aaaaccccgg ggcgcgatag cgaagtggcc
     2641 agttcaatcg aaaaagccgt tgccggtgcg ccgggtaaat caggttacaa cgttccggca
     2701 atcctcgaag ccttaggcgg tgccgacaat attgttagcc tcgataactg cattacccgt
     2761 ctgcgtttgt ctgtgaaaga tatgtcgctt gttaatgtgc aggcactgaa ggacaatcgg
     2821 gcaattggcg tagtacaact taatcaacat aacctgcagg ttgttatcgg gccacaagtt
     2881 cagtcagtaa aagatgaaat ggccggtctg atgcatactg tccaggcata aggataagat
     2941 atgttcgatt tttcaaaggt cgtggatcgt catggcacat ggtgtacaca gtgggattat
     3001 gtcgctgacc gtttcggcac tgctgacctg ttaccgttca cgatttcaga catggatttt
     3061 gccactgccc cctgcattat cgaggcgctg aatcagcgcc tgatgcacgg cgtatttggc
     3121 tacagccgct ggaaaaacga tgagtttctc gcggctattg cccactggtt ttccacccag
     3181 cattacaccg caattgatac gcagacggtg gtgtatggcc cttctgtcat ctatatggtt
     3241 tcagaactga ttcgtcagtg gtctgaaaca ggtgaaggcg tggtgatcca cacgcccgcc
     3301 tatgacgcat tttacaaggc cattgaaggt aaccagcgca cagtaatgcc cgttgcttta
     3361 gagaagcagg ctgacggctg gttttgcgat atgggcaagc tggaagccgt tttggcgaaa
     3421 ccagaatgta aaattatgct cctgtgtagc ccacagaatc ctaccgggaa agtgtggacg
     3481 tgcgatgagc tggagatcat ggctgacctg tgcgagcgtc atgatgtgcg ggttatttcc
     3541 gatgaaatcc atatggatat ggtctggggc gagcagccgc atattccctg gagtaatgtg
     3601 gcgcggggag actgggcgtt gctgacgtcg ggatcgaaaa gtttcaatat tcccgcgctg
     3661 accggtgctt acgggattat agaaaatagc agcagccgtg atgcctattt atcggcactg
     3721 aaaggacgtg atgggctttc ttccccttcg gtactggcgt taactgccca tatcgccgcc
     3781 tatcagcaag gtgcaccgtg gctggatgcc ttacgcgtct atctgaaaga taacctgacg
     3841 tatatctcag ataaaatgaa cgccgcgttt cctgaactca actggcagat cccgcaatcc
     3901 acttatctgg cctggcttga tctacgtccg ttgaatattg acgacaacgc gttgcaaaaa
     3961 gcgcttatcg agcaagaaaa agtcgcgatc atgccggggt atacctatgg tgaagaaggt
     4021 cgtggttttg tccgtctcaa tgccggctgc ccacgttcga aactggaaaa aggtgtggct
     4081 ggattaataa acgccatccg cgctgttcgc taaccccaat tgcgcaacgt aaaaaatcgt
     4141 tgcgcaatcg tggattttta ccctgctttg tttttataat ggtgcgcact tttatatcca
     4201 gaaaaagagt gcgaccatga ttgataccac cctgccatta actgatatcc atcgccacct
     4261 tgatggcaac attcgtcccc agaccattct tgaacttggc cgccagtata atatctcgct
     4321 tcctgcacaa tccctggaaa cactgattcc ccacgttcag gtcattgcca acgaacccga
     4381 tctggtgagc tttctgacca aacttgactg gggcgttaaa gttctcgcct ctcttgatgc
     4441 ctgccgccgc gtggcatttg aaaacattga agatgcagcc cgtaacggcc tgcactatgt
     4501 cgagctgcgt ttttcaccag gctacatggc aatggcacat cagctgcctg tagcgggtgt
     4561 tgtcgaagcg gtgatcgatg gcgtacgtga aggttgccgc acctttggtg tgcaggcgaa
     4621 gcttatcggc attatgagcc gaaccttcgg cgaagccgcc tgtcagcaag agctggaggc
     4681 ctttttagcc caccgtgacc agattaccgc acttgattta gctggtgatg aacttggatt
     4741 cccgggaagt ctgttccttt ctcacttcaa ccgcgcgcgt gatgcgggct ggcatattac
     4801 cgtccatgca ggcgaagctg ccgggccgga aagcatctgg caggcgattc gtgaactggg
     4861 tgcggagcgt attggacatg gcgtaaaagc cattgaagat cgggcgctga tggattttct
     4921 cgccgagcaa caaattggta ttgaatcctg tctgacctcc aatattcaga ccagcaccgt
     4981 agcagagctg gctgcacatc cgctgaaaac gttccttgag catggcattc gtgccagcat
     5041 taacactgac gatcccggcg tacagggagt ggatatcatt cacgaatata ccgttgccgc
     5101 gccagctgct gggttatccc gtgagcaaat ccgccaggca cagattaatg gtctggaaat
     5161 ggctttcctc agcgcagagg aaaaacgcgc actgcgagaa aaagtcgccg cgaagtaaca
     5221 aaaatggatg gtgcaatttg caccatccat ttttcacgca aggcacaacg tcgcgcgatg
     5281 tttggcggat tcgatgccca gctcaatcaa ctccattacc tggattgcct ggcttgccgg
     5341 aaccggattt tcaccatcgc catttaacgc atcacgaatc gccgcatagt aagccggata
     5401 attcccaggc accgtcaaca gcgtttcttc gacacgttcc tcaccttcca cgcgggtaag
     5461 tacgccatca cgcatatcgt agccccagtc ttcctgcggc agacgctcgc catttttcag
     5521 acgttcttcc tgtggatcga ggccatattt cacacaactg cctcgggatc catgcacgat
     5581 ataccgtgct gactcagcgg ctgccagcat ggtaccgtgt aaaatgactc gccgctgtgg
     5641 ataggacaag atggcgtgga aataatcggt cgactgcgct ccgggccgta actgtgccaa
     5701 atctaccgtc atgctgaccg gtaaaccaaa tagcgtaatg gcctgatcaa gaagatgcgg
     5761 tgctaaatcg taccagatac cgctgcctgg gccgccctgt tcacgccaac gatcgcgcac
     5821 ctgcggacgg aagcggtcaa aatgagactc aaagtaagca acttcaccca gcacgccttc
     5881 cgcgagcaaa ccttttagcg tcaagaaatc gctatcccaa cgacggttat ggaatacaga
     5941 cagcacacgc cccaggcttt ttgccagcgc atccagctct cgcgcttgtg acagtgtcac
     6001 ggtaaagggt ttatcaacga ccacatgttt acccgcctca agcgccgctt tggctaacgg
     6061 gaaatgggta tcgttgggtg taggaatgac aatcaggtct atgttgggat cgttaaacag
     6121 atgcttcggc tcagagacaa ccgtaaccgt tggccagtcg gcttttactt ttgtttcatc
     6181 actgctggag attaccgcca gttccagccc gggcgtgccc gcaatcaggg gcgcatggaa
     6241 ggttttgctc gcataaccat acccaatcaa cccaacacgg atgttgtcgc tcatgtcatt
     6301 gcctctcatt gaagtatgat ggctatttga cactatcctt tacccacgct caacagttta
     6361 ataacctgcc agcaataagg gatgttgttt aacttaagtc aaaaaaaata gcgaattttc
     6421 caacgacaaa agctaaatat cgcaaaaacc tcagtaaaaa tcttgctgga gctattattg
     6481 ctaagtaaca tttaccccct gaagttaatg gatcaatcaa gagagatgtg ggctgtaatg
     6541 aatcgtctta ttgaattaac aggttggatc gttcttgtcg tttcagtcat tcttcttggc
     6601 gtggcgagtc acattgacaa ctatcagcca cctgaacaga gtgcttcggt acaacacaag
     6661 taagctctgc acttgtggag cgacatgctg cccgtccggg tgcatgtttt cacttgtcgg
     6721 atattaaacc aggaatttat tatcttgttc gatgttgttg gtgattgtca gggatagtaa
     6781 agcgcgttac tcttttcaga gcagtttttc atccctgaat atttgccatc agcgcattct
     6841 tattattgta ataacacaag cgttcacttt tggtgaaatt ttatcatcac tctacccctt
     6901 tggtattaat tattttatgg accttttatg actgttcagg actacttatt aaaatttcgc
     6961 aaaatcagtt cactcgaaag tctggaaaaa ctctacgacc atcttaatta caccctgacg
     7021 gacgatcagg aactgatcaa tatgtatcgt gctgccgatc accgtcgcgc agagctggtt
     7081 tctggcgggc gtttgtttga cctcggccag gtaccgaagt ccgtctggca ctatgtccaa
     7141 taaagaaagt agcgatcggt tataaaagcc ttataataac gcctctaaaa ttagatttgc
     7201 ctgtttgcgt cgctattctg gagaacctat gaccaccaca acgccacaac gtattggagg
     7261 ctggttgctt ggcccgttgg cctggctttt agtcgctctg ttgagtacga cgctggcgtt
     7321 gttgctgtat accgctgcgt tatcttctcc gcaaacattt caaacgctcg gcggacaagc
     7381 actgacgaca caaatcttat ggggcgtttc ttttattacc gctatcgcca tgtggtatta
     7441 cacactgtgg ttgaccatcg ctttctttaa acgtcggcgt tgtgttccta aacactatat
     7501 tatctggctg cttatttccg tattgctggc agtaaaagca ttcgcttttt cacccgtgga
     7561 agatggcata gccgttcgcc agttgctgtt tactttgttg gcaaccgcat tgatcgtccc
     7621 ctatttcaaa cgctcgtcgc gggtaaaagc gacgtttgtg aatccgtaat aaccttacag
     7681 ttaacctgtt gtcgcctgct ctggattaac ggataatagg cggctttttt atttcaggcc
     7741 gaaaaatgac tgactacctg ttactgtttg tcggaactgt actggtcaat aactttgtac
     7801 tggtcaagtt tctcggtctc tgtccgttta tgggggtttc caaaaaactg gaaaccgcga
     7861 tgggcatggg gctggcaaca acgtttgtga tgacgctggc gtctatttgc gcctggctta
     7921 tcgatacgtg gattttgatc ccacttaatc tgatttacct gcgcaccctg gcatttattc
     7981 tggtgattgc tgtggtcgtg cagttcaccg agatggtggt gcgcaaaacc agcccggtgc
     8041 tttaccgtct gctggggatt tttttgccgc ttatcaccac caactgtgca gtgctcggcg
     8101 tggcgttgct gaatatcaat ctcgggcaca atttcttgca gtcggcgctg tacggttttt
     8161 ccgccgctgt cggtttctcg ctggtgatgg tgctcttcgc cgccatccgc gaacgccttg
     8221 ctgtggctga tgtcccggca ccttttcgcg gtaatgccat tgcgttaatt accgcaggtc
     8281 ttatgtctct ggcctttatg ggctttaatg gtttggtgaa gttgtaatga atgctatctg
     8341 gattgccgtt gccgccgtga gcctgctggg cctggcgttt ggcgccattc tgggttatgc
     8401 ctcccgccgt tttgcggtgg aagacgatcc ggtcgttgag aaaattgacg aaatcttacc
     8461 gcagagccag tgtggtcagt gcggttatcc cggctgtcgc ccctacgcgg aagccatcag
     8521 ctgtaacggt gaaaaaatca accgttgcgc cccaggtggc gaagctgtga tgctaaaaat
     8581 tgccgagttg cttaatgtcg agccgcagcc gctggatggc gaagcgcaag agctaacgcc
     8641 tgcgcggatg gtggcggtta ttgatgaaaa taactgtatt ggctgcacta aatgtattca
     8701 ggcttgtccg gtagacgcca tcgttggcgc tacccgtgcc atgcatacgg taatgagtga
     8761 tctctgtacg ggctgcaatt tatgtgttga tccgtgcccg acgcactgca tctcgttgca
     8821 accggtcgca gaaacacctg actcctggaa atgggatctg aacaccattc ccgtgcgtat
     8881 cattcccgtg gaacaccatg cttaagttat tctctgcatt cagaaaaaat aaaatctggg
     8941 atttcaacgg cggcatccat ccaccggaga tgaaaaccca gtccaacggt acacccctgc
     9001 gccaggtacc actggcgcag cgttttgtta ttccactgaa acagcatatt ggcgctgaag
     9061 gtgagttgtg cgttagcgtc ggcgataaag tattgcgcgg ccagccgctt acccgtggtc
     9121 gcggcaaaat gctgcctgtt cacgcgccca cctcgggtac cgttacggct attgcgcccc
     9181 actctacggc tcatccttca gctttagctg aattaagcgt gattattgat gccgatggtg
     9241 aagactgctg gatcccgcgc gacggctggg ccgattatcg cactcgcagt cgcgaagagt
     9301 taatcgagcg catacatcag tttggtgttg ccgggctggg cggtgcagga ttcccgacag
     9361 gcgttaaatt gcagggtggc ggagataaga ttgaaacgtt gattatcaac gcggctgagt
     9421 gcgagccgta cattaccgcc gatgaccgtt tgatgcagga ttgcgcggct caggtcgtag
     9481 agggtattcg cattcttgcg catattctgc agccacgcga aattcttatc ggcattgaag
     9541 ataacaaacc gcaggcgatt tccatgctgc gcgcggtgct ggcggactct aacgatattt
     9601 ctctgcgggt gattccaacc aaatatcctt ctggcggtgc taaacaatta acctacattc
     9661 tgaccgggaa gcaggttcca catggcgggc gttcatccga tatcggcgta ttaatgcaaa
     9721 acgtcggcac tgcttatgca gtgaaacgtg ccgttattga tggcgagccg attaccgagc
     9781 gtgttgtaac cctgactggc gaagcaatcg ctcgcccggg caacgtctgg gcacggctgg
     9841 ggacgccagt gcgtcattta ttgaatgatg ccggattctg cccctctgcc gatcaaatgg
     9901 tgattatggg tggcccgcta atgggcttta ccttgccatg gctggatgtc ccggtcgtaa
     9961 agattaccaa ctgtctgttg gctccctctg ccaatgaact tggcgaacca caggaagaac
    10021 aaagctgcat ccggtgtagc gcctgtgctg acgcctgccc tgcggatctt ttgccgcaac
    10081 agttgtactg gttcagcaaa ggtcagcaac acgataaagc taccacgcat aacattgctg
    10141 attgcattga atgtggggct tgcgcgtggg tttgcccgag caatattccc ctggtgcaat
    10201 atttccgtca ggaaaaagct gaaattgcgg ctattcgtca ggaagaaaag cgcgccgcag
    10261 aagccaaagc gcgtttcgaa gcgcgccagg ctcgtctgga gcgcgaaaaa gcggctcgcc
    10321 ttgaacgaca taagagcgca gccgttcaac ctgcagccaa agataaagat gcgattgctg
    10381 ccgctctggc gcgggtgaaa gagaaacagg cccaggctac acagcctatt gtgattaaag
    10441 cgggcgaacg cccggataac agtgcaatta ttgcagcacg ggaagcccgt aaagcgcaag
    10501 ccagagcgaa acaggcagaa ctgcagcaaa ctaacgacgc agcaaccgtt gctgatccac
    10561 gtaaaactgc cgttgaagca gctatcgccc gcgccaaagc gcgcaagctg gaacagcaac
    10621 aggctaatgc ggaaccagaa gaacaggt
//
LOCUS       DAJVQG010000136        10646 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJVQG010000136 DAJVQG010000000
VERSION     DAJVQG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10646
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.136"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..729
                     /gene="narU"
                     /locus_tag="OQM83_003190"
     CDS             <1..729
                     /gene="narU"
                     /locus_tag="OQM83_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCS1521384.1"
                     /translation="LAIATIAAWSGMNDIASSRASIDDQLPVLQRLHLWLLSLLYLAT
                     FGSFIGFSAGFAMLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLIN
                     FIFMAIFSALLFLTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIY
                     RVKMKGGSDEQAQKEAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKV
                     FLIFYIVCVLLTWLVYGRRKFSQK"
     gene            811..4551
                     /gene="narZ"
                     /locus_tag="OQM83_003191"
     CDS             811..4551
                     /gene="narZ"
                     /locus_tag="OQM83_003191"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HCS1521385.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            4548..6092
                     /gene="narH"
                     /locus_tag="OQM83_003192"
     CDS             4548..6092
                     /gene="narH"
                     /locus_tag="OQM83_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCS1521386.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6092..6787
                     /gene="narW"
                     /locus_tag="OQM83_003193"
     CDS             6092..6787
                     /gene="narW"
                     /locus_tag="OQM83_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCS1521387.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            6784..7464
                     /gene="narI"
                     /locus_tag="OQM83_003194"
     CDS             6784..7464
                     /gene="narI"
                     /locus_tag="OQM83_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCS1521388.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            7543..8436
                     /locus_tag="OQM83_003195"
     CDS             7543..8436
                     /locus_tag="OQM83_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HCS1521389.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(8532..9377)
                     /gene="nhoA"
                     /locus_tag="OQM83_003196"
     CDS             complement(8532..9377)
                     /gene="nhoA"
                     /locus_tag="OQM83_003196"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HCS1521390.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            9550..10119
                     /locus_tag="OQM83_003197"
     CDS             9550..10119
                     /locus_tag="OQM83_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCS1521391.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(10123..10350)
                     /gene="pptA"
                     /locus_tag="OQM83_003198"
     CDS             complement(10123..10350)
                     /gene="pptA"
                     /locus_tag="OQM83_003198"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HCS1521392.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(10450..>10646)
                     /locus_tag="OQM83_003199"
     CDS             complement(10450..>10646)
                     /locus_tag="OQM83_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1521393.1"
                     /translation="VVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKM
                     RKAAMGRNYLASVLTGSGLS"
BASE COUNT         2489 a         2796 c         2857 g         2504 t
ORIGIN      
        1 ctggcgattg ccacgatcgc cgcctggtca gggatgaatg atatcgccag ttcacgcgcc
       61 tcaattgacg accagctccc tgtcttacaa cgcctgcatc tctggctgct gagcctgctt
      121 taccttgcca ccttcggttc gtttatcggt ttttctgcgg gttttgccat gctggcaaaa
      181 acccagttcc cggatgtgaa tattctgcgc ctggcgttct ttggcccatt tatcggtgcc
      241 atcgcgcggt cggttggtgg tgctatttcc gataaattcg gcggcgtgcg ggtgacgttg
      301 atcaacttta tttttatggc gattttcagt gccctgctgt tccttacctt accgggcaca
      361 ggctccggta atttcatcgc cttttacgcc gtatttatgg ggctgtttct gactgcgggt
      421 ctgggaagtg gttctacttt ccagatgatc gccgtcatct ttcgccagat aaccatttat
      481 cgggtgaaga tgaaaggcgg tagtgatgag caagctcaga aagaagccat caccgaaacg
      541 gcggcggctc tgggctttat ctcagccatt ggcgcagtgg gcggcttttt tattccgcag
      601 gcgtttggca tgtcgctcaa tatgaccggc tctccggtcg gcgcgatgaa agtgttttta
      661 atcttctaca tcgtttgtgt gctgctgacc tggctggttt atggtcggcg gaagttcagc
      721 caaaaataag agatcgatgc ctgatgtaca accgtgtcgc atccgacaag gcatcagatc
      781 ccaattcatt cctggagcag gagtcatgtc atgagtaaac ttttggatcg ctttcgctac
      841 ttcaaacaaa agggcgaaac ctttgccgat ggtcacggac aggtgatgca tagcaaccgc
      901 gactgggagg acagctatcg ccagcgttgg cagttcgaca aaattgtgcg ttccacccac
      961 ggtgttaact gtacaggctc ctgtagctgg aaaatctacg ttaaaaatgg tctggtgacc
     1021 tgggaaatcc aacagaccga ctacccgcgc actcgccctg accagcccaa tcatgaacct
     1081 cgcggctgcc cgcgtggcgc aagttactcc tggtatctct acagcgctaa ccgcctgaaa
     1141 tacccgctca ttcgtaaacg actgattgaa ctgtggcgtg aagctctcaa gcaacacagc
     1201 gatccggtac tggcgtgggc atcgattatg aacgatccgc aaaagtgctt aagctacaaa
     1261 caagtgcgtg ggcgcggcgg gtttatccgc tccaactggc aggaacttaa ccaactgatt
     1321 gccgccgcta acgtctggac catcaaaatc tatggtccgg atcgcgttgc cggtttctcg
     1381 ccgatcccgg cgatgtcgat ggtgtcatac gctgccggaa cgcgttatct gtcgctgtta
     1441 ggcggcacct gtttaagctt ctacgactgg tattgcgacc tgccgcccgc ctcgccgatg
     1501 acctggggcg aacaaaccga cgtgccggaa tctgccgact ggtataactc cagttacatc
     1561 atcgcctggg ggtctaacgt accgcagaca cgtacgccgg acgcccactt ctttaccgaa
     1621 gtacgttaca aaggcaccaa aactatcgcc attacccctg actactctga agtggccaaa
     1681 ttgtgcgacc agtggctggc accgaaacaa ggcactgata gcgccctggc gatggcaatg
     1741 ggccatgtga ttttaaaaga gtttcatctc gataatccca gcgactactt tatcaactac
     1801 tgccgccgct atagcgacat gccgatgctg gtaatgctgg aacctcgcga cgatggtagc
     1861 tacgtacccg ggcgcatgat ccgcgcgtct gacctggtag atggactagg cgaaagcaat
     1921 aatccgcagt ggaaaaccgt ggcggttaat accgcaggtg aattggtagt gccgaatggt
     1981 tcgattggtt tccgctgggg agaaaaaggc aaatggaatc tggaatccat tgccgccggt
     2041 aaggaaaccg aattgtcgtt aaccctgctc ggtcaacatg acgctgttgc aggcgtggcc
     2101 ttcccctact ttggcggcat tgaaaatccg cattttcgca gcgtaaaaca caatccggtg
     2161 ctggtgcgcc aattgcccgt taaaaacctg acgttagtcg atggcaacac ctgtccggtg
     2221 gtcagcgttt atgatttggt gctggcgaat tacggcctcg atcgcgggct ggaagatgaa
     2281 aacagcgcga aagattacgc tgaaatcaaa ccgtacaccc cagcctgggg tgagcaaatt
     2341 accggcgtac cgcgccagta tattgaaacc atcgcccgcg aatttgccga taccgcccat
     2401 aaaacgcatg gacgctcaat gattatcctc ggcgctggcg tcaaccactg gtatcacatg
     2461 gatatgaact accgtgggat gatcaatatg ctgatcttct gcggttgtgt cgggcaaagc
     2521 ggcggcggct gggcgcacta tgtcggtcag gaaaaactgc gcccacaaac cggctggttg
     2581 ccgctggcct ttgcgctcga ctggaaccgc ccaccgcgcc agatgaacag tacctcgttt
     2641 ttctacaatc attccagcca atggcgctat gaaaaagtct ctgctcagga gttactttca
     2701 ccgcttgccg atgccagtaa gtacagcggt catctgattg atttcaacgt tcgcgccgaa
     2761 cgcatgggct ggctaccttc tgcgccgcag ctggggcgta acccgctcgg gattaaagct
     2821 gaagccgaca aggccggatt atcccccaca gaatttaccg cccaggcgct gaaatcgggc
     2881 gatttacgta tggcctgcga acaaccagat agcggcagca atcatccgcg taatttgttt
     2941 gtctggcgct ctaacctgct tggctcctcc ggcaaaggcc acgagtatat gcagaagtat
     3001 ctgctgggga ccgaaagcgg gattcagggc gaggaactcg gtgccagcga cgggatcaaa
     3061 ccggaagaag tcgagtggca aactgcggcg attgaaggca agctcgacct gctggtgacg
     3121 ctcgacttcc gcatgtccag tacctgcctg ttctccgata tcgttctgcc caccgccacc
     3181 tggtacgaaa aagacgatat gaacacctcg gatatgcatc cgtttattca tccgctttct
     3241 gcggcggtcg atccggcgtg ggaatcacgc agcgactggg aaatctacaa aggtatcgcc
     3301 aaagcatttt cgcaagtgtg cgtgggtcat cttggcaaag aaaccgacgt ggtattacaa
     3361 cccctactgc atgactctcc ggcagagctc tcacagccgt gtgaagtgct cgactggcgc
     3421 aaaggcgaat gcgatctgat tccaggcaaa accgcgccga atattgtggc ggtggagcgc
     3481 gactaccctg ctacgtatga acgctttacc tcgctcgggc cattgatgga caaacttggc
     3541 aatggtggta aagggatttc gtggaatacc caggatgaaa tcgatttcct cggcaaactc
     3601 aattacacca agcgtgatgg cccggcacag gggcgtccgc tgattgacac cgccattgac
     3661 gcttcagaag tgattctggc actggcaccg gaaaccaacg gtcatgttgc agtcaaagcg
     3721 tggcaggcgc tgggcgagat caccgggcgc gaacataccc atctggcgct gcacaaagag
     3781 gacgagaaga ttcgctttcg cgatattcag gcgcagccgc gtaaaattat ctccagcccc
     3841 acatggtccg gtctggaaag cgatcacgtc tcctataatg cgggatacac caacgttcat
     3901 gagttaattc cgtggcgcac gctgtcggga cgccagcagc tctatcagga tcatccgtgg
     3961 atgcgtgctt ttggtgaaag cctggtggct tatcgcccgc ctatcgacac ccgtagcgtc
     4021 agtgagatgc gccagatccc gccaaacggc ttcccggaaa aagcacttaa cttcctgacg
     4081 ccgcaccaga aatggggcat tcactcaacc tacagtgaaa acctgctaat gctgacgctc
     4141 tctcgcggtg gaccgattgt ctggatcagc gaaaccgatg cccgagaact gaccattgtc
     4201 gataacgact gggtggaagt ctttaacgcc aacggcgcgc tgacggctcg tgcggtggtc
     4261 agccaacgtg taccgccggg tatgaccatg atgtatcacg cccaggaacg cattatgaat
     4321 attcctggtt cggaagtaac tggcatgcgc ggcggcattc ataactcggt cacccgtgtt
     4381 tgcccgaaac cgacgcatat gattggcggt tacgcgcagc tggcctgggg ctttaactac
     4441 tacggcaccg tcggctcgaa ccgcgacgaa ttcatcatga tccgcaagat gaagaacgtt
     4501 aactggctgg atgatgaagg tcgcgatcag gtacaggagg cgaaaaaatg aaaatacgtt
     4561 cgcaagtcgg catggtgctt aacctcgaca aatgtatcgg ttgccatacc tgttcggtga
     4621 cctgtaaaaa cgtctggacc ggacgcgaag gcatggagta cgcatggttt aacaacgtcg
     4681 aaaccaaacc gggcattggt tatccgaaaa actgggaaga tcaggaagag tggcaaggcg
     4741 gctgggtccg cgatgtgaat ggcaagatac gcccgcgtct gggcagcaag atgggcgtaa
     4801 taaccaaaat cttcgccaac ccggtggtgc cgcagattga tgattactac gaacctttca
     4861 ccttcgacta cgaacatttg catagcgcac cggaaggcaa acatatccct actgctcgcc
     4921 cgcgttcgct gattgacggt aagcggatgg acaaagtgat ctgggggcca aactgggaag
     4981 aactgctggg cggcgagttt gaaaaacgtg cccgcgaccg caacttcgag gccatgcaaa
     5041 aggagatgta cgggcagttt aaaaacacct tcatgatgta cctgccgcgc ctgtgcgaac
     5101 actgcctcaa tcccagttgc gtggcgacct gcccaagcgg cgctatctac aaacgcgaag
     5161 aagacggcat tgtgctgatt gatcaggata aatgccgtgg ctggcgtttg tgcattagcg
     5221 gttgtccgta caaaaaaatc tacttcaact ggaaaagcgg caagtcagaa aaatgtatct
     5281 tctgttatcc gcgaattgag tcaggccaac ccaccgtgtg ctcagaaacc tgcgtgggtc
     5341 gcatccggta tctgggcgtg ctgctttacg acgccgaccg cattgaggaa gcggcgagca
     5401 ccgagcgcga agttgacctc tatgaacgtc agtgcgaagt gttcctcgat ccacacgatc
     5461 cctcagtgat cgaggaagcc ctgaaacaag gtattccaca aaacgtgatt gaagctgccc
     5521 agcgttcgcc tgtctacaaa atggcgatgg actggaaact ggcgctaccg ctgcaccctg
     5581 aatatcgcac cctgccaatg gtctggtacg ttcctccgct gtcgccgatt cagtcctacg
     5641 cagatgcggg cggtttgccg aaaagcgaag gcgtgctgcc cgccatcgaa agcctgcgta
     5701 ttccggtgca atatctcgcc aatatgttga gtgccggcga taccggtccg gtactgcggg
     5761 cgctgaaacg gatgatggcg atgcgccact atatgcgttc acaaaccgtg gaaggcgtta
     5821 ctgatactcg tgccatcgac gaagtaggcc tgagcgtcgc ccaggtcgaa gaaatgtatc
     5881 gctatctcgc cattgccaac tatgaagatc gttttgttat cccgaccagc catcgggaaa
     5941 tggcgggcga tgccttcgca gaacgcaacg gctgcggttt tacctttggc gacggttgcc
     6001 acggttcgga cagtaaattc aacctgttca acagtagccg tatcgatgcc atcaacatca
     6061 ccgaagtgcg cgacaaagcg gagggcgaat aatgcagatc ctcaaagtga tcggcctgtt
     6121 gatggagtat ccggatgagc tgttgtggga atgcaaggag gacgcgctgg cgttgatccg
     6181 ccgcgacgcg ccgatgctta cggatttcac ccacaacctg cttaacgcgc cgctgctgga
     6241 taaacaggcc gaatggtgcg aagtgtttga ccgcgggcgc accacgtcgc tgctgctgtt
     6301 cgaacatgtt catgccgagt cccgcgatcg cggccaggca atggtggacc tgctggcgga
     6361 gtatgaaaag gtcggcctgc agctggattg tcgggaactg cccgattatc taccgctgta
     6421 tctggagtat ttaagcgtac tgcctgacga tcaggcgaaa gaaggattgc tcaatgttgc
     6481 gccgatcctc gccctgcttg gcggtcgctt aaaacaacgc gaggcaccgt ggtacgcgtt
     6541 gtttgatgct ctgctgcaac tggcgggaag tatactttca agtgacagtg tcacaaaaca
     6601 agtaaacagc gaagagcgcg atgacacccg ccaggcgctt gatgcagtgt gggaagagga
     6661 acaggtgaag tttattgaag ataacgccac ggcgtgtgac agctcgccgc ttaatcaata
     6721 tcagcgacgc tttagccagg atgtcgcgcc gcagtatgtc gacatcagtg cgggaggtgg
     6781 gaaatgattc agtatctgaa cgtctttttt tacgatatct acccgtacat ttgtgcgacg
     6841 gtgtttttcc tcggcagctg gctgcgctac gactacgggc agtacacctg gcgcgcctcc
     6901 tcaagtcaaa tgctcgataa acgcgggatg gtgatatggt cgaatctgtt ccatatcggc
     6961 attttgggga ttttcttcgg gcacttgttc ggcatgttaa cgccgcactg gatgtacgcg
     7021 tggtttttgc cagtggcagc gaaacaactc atggcgatgg tgctcggcgg tatttgcggt
     7081 gtgttgacgt tgattggcgg cgcaggactg ctgtggcgca gtctgacaaa tcagcgcgtg
     7141 cgtgccactt ccactacgcc ggatatcatc atcatgagca ttttgctgat ccagtgtctg
     7201 ttggggctaa gtaccatacc gttttcagca cagtatcctg atggtagcga aatgatgaag
     7261 ctggtgggtt gggcgcaaag cattgtcact ttccgtggtg gatcgtcaga gatgctcaac
     7321 ggcgtagcgt tcgtcttccg cctgcatctg gtgttgggaa tgaccatctt cctgctcttc
     7381 ccgttcaccc gactggtgca tgtgtggagc gcgccgtttg agtactttac tcgtcgatat
     7441 caaattgtgc gttcgcggcg ataatttcac cctggccgga tagcccttcg ctctccggcc
     7501 acttgcaggc tatgatagtt ctccagtcac aggagaacgt taatgaaacc gcaggtgtac
     7561 cacgtcgatg cttttacctc tcaaccattt cgcggcaatt ctgccggtgt tgttttcccc
     7621 gccgataatc ttagcgaagc acaaatgcag cttatcgccc gcgagttagg ccattcggaa
     7681 actgcttttc tgctgcacag cgacgacagc gatgtgcgca tccgctactt tacgccaacg
     7741 gttgaagtgc cgatttgcgg tcacgcgaca gtagctgcgc actatgtacg tgcgaaggtg
     7801 ttaggtttag gaaattgcac gatctggcaa acatcgctgg caggaaaaca tcgcgtgact
     7861 atcgaaaagc acaacgatga ttatcgtatt tcgctggaac aaggtacgcc gggctttgaa
     7921 ctgccgctgg agggtgaagt acgcgcggcg attatcaacg cattgcatct tactgaagac
     7981 gatattctgc cgggtttgcc gatccaggtc gcgacgaccg ggcattctaa agtgatgatc
     8041 ccattgaagc cggaagtgga tatcgacgct ctttcgcctg accttaatgc gctgaccgct
     8101 atcagtaaac agattggctg caatggtttc ttcccgttcc agatccgtcc aggcaaaaac
     8161 gaaaccgatg gtcgtatgtt ctcgcctgcg attggtattg tggaagatcc ggtgactggc
     8221 aatgccaatg gcccgatggg tgcatggttg gtacatcaca acgtattgcc ccacgatggc
     8281 aacgtgttgt gtgttaaagg ccatcagggg cgcgcattgg ggcgtgacgg catgattgaa
     8341 gtgacggtga caattcgtga taaccaaccg gaaaaagtca ccatttcagg cacagcggtg
     8401 attttgtttc atgccgaatg ggcaattgaa atctgaaatc gttgcctgat gcgcttcgcc
     8461 tgtcaggtct acaaccgggc taatgtatgc cggataaggc gtttacgccg catccggcaa
     8521 cctgacataa attattttcc cgcctccgga tgcgtatcaa acgccgccat caccagcgct
     8581 aactcatcca cagtaaagcc atgtttcgca tcatccacgc ccagaccaaa ctgttcctgc
     8641 atcacggcat ataacgacgc cacatccggt agatttcgct gctccaccgc gtgcccattt
     8701 tcataatggg taaaatgaaa attggtcagt gtcagcttgc cgccgtccgg caaatggcgg
     8761 cacatcagca aatgatggcg aaaatgcgac tgcggccagt gcgccgacca gaaattgccc
     8821 atcacataat cgctttgttg ctgctcgcag agatcgaagc ggtacatcgg ctgccaatgc
     8881 tgatgatgat taaactgcaa cacccaatca tcaccctcct gcaacaaccg atactctccg
     8941 tgtggcgtgg tctgcacgag atcggaaact aaacgaatcg gcgcggttag cgtctgccca
     9001 ccgaaaccga catcagcaat ccatttttcc ccttccagtt ccaccaacag caaacgatgg
     9061 gtgcgcggcg gtaatgctgg cggatttgat aacactacgc gccctaacaa gctgcgaacg
     9121 ttaaacccca gctcgcgtaa cacccgctca aacacgccat tctgctcaaa acagtaaccg
     9181 ccacgacggg ctatcaccag tttctcttcc agcgattgat catcaagctg tatttccctc
     9241 ggcagcaaaa cgtcaaggtt ttcaaacgga atggtgcaat tgtgtttcag gtgcaatgca
     9301 cgaagcgtat caatattgac cgcagcagct cccgaccagt taatacgggc aaaatagtga
     9361 ttcagaatgg gcgtcatcag gcgtttccct tagtggtttt ctcaacttat aacctggaat
     9421 agacgccgca ccggctattt tgtgcgatcg cggacatgca tcacgtttgc aaatcgccaa
     9481 tcccgttaat ttcgttactt attgcgttac ggactacgct taaaaaaacc ttcttcgcga
     9541 ggcgatacca tgagccgatt catccccata gaattacacc atgccagccg tctgttaaat
     9601 catggcccaa ctgtcttgat caccagtttc gatgagcaat cccagcggcg taacattatg
     9661 gctgcagcct ggtcaatgcc ggtggagttt gaacctccac gcgtggcaat tgtggtagat
     9721 aaatcgacat ggaccaggga gttgattgaa cgtaacggta agtttggcat cgttatcccg
     9781 ggcgttgcag caactaactg gacgtgggcg gtgggaagtg tgtcggtgcg tgaagaagat
     9841 aaatttaatt gctatggcat tccggtggtg aaaggtccgg tgcttggttt gccacttgtt
     9901 gaagagaaat gcctggcgtg gatggagtgc cgcctgctac ctgcgacttc tgcgcaacaa
     9961 aaatacgaca cgctgtttgg cgaggtggta tcagcagcgg cagacgcacg ggtatttgtc
    10021 gaaggccgct ggcagtttga tgatgataag ctcaatacgt tgcatcattt aggtgctggc
    10081 acgtttgtta ctagcggcaa gcgtgttacg gcaggttaag cattacatgc tgtaaccggg
    10141 tttctttatc aaagcctcca tttggggctc gatttcggca tcccagatag cttgccaaga
    10201 ttctggttga atctgctgta gagcaatgct tatcgagcta tctttactgt tcagatggcg
    10261 aataataacg tcggtaatat ctgcagcaag tgctgctttt tgttgttcgt ccagttcacg
    10321 cggaaagcat ttaatgtcga tgtgcggcat gattacgctc cttttgaaag atgtcagtaa
    10381 aattaacaca cattgtgaat gtcatgtgtc cggtaaagcg tttatacgac aataatgccc
    10441 agggcaagat tacgaaagcc cgctccccgt aaggactgac gccaggtagt ttctgcccat
    10501 ggctgctttt cgcatcttac gtcttaaccc tgccttgaat accttatcat tcgtcaaaat
    10561 attaatagca atgtgccgta tccctgaaaa taattctgct gcatttcctc ttcttatttt
    10621 gcagtcgtct tcattcatta ccacgt
//
LOCUS       DAJVQG010000137        10266 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJVQG010000137 DAJVQG010000000
VERSION     DAJVQG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10266
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.137"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..421
                     /locus_tag="OQM83_003200"
     CDS             <1..421
                     /locus_tag="OQM83_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCS1521394.1"
                     /translation="VARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQDMSIMHEET
                     FGPVLPVVAFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENF
                     EAMQGFHAGWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(463..1464)
                     /gene="gap"
                     /locus_tag="OQM83_003201"
     CDS             complement(463..1464)
                     /gene="gap"
                     /locus_tag="OQM83_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCS1521395.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            1653..2183
                     /gene="cybB"
                     /locus_tag="OQM83_003202"
     CDS             1653..2183
                     /gene="cybB"
                     /locus_tag="OQM83_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCS1521396.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            2428..2601
                     /locus_tag="OQM83_003203"
     CDS             2428..2601
                     /locus_tag="OQM83_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521397.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(2673..2822)
                     /gene="hokB"
                     /locus_tag="OQM83_003204"
     CDS             complement(2673..2822)
                     /gene="hokB"
                     /locus_tag="OQM83_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCS1521398.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            3221..4861
                     /gene="trg"
                     /locus_tag="OQM83_003205"
     CDS             3221..4861
                     /gene="trg"
                     /locus_tag="OQM83_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCS1521399.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(4899..5822)
                     /locus_tag="OQM83_003206"
     CDS             complement(4899..5822)
                     /locus_tag="OQM83_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1521400.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            6039..7382
                     /locus_tag="OQM83_003207"
     CDS             6039..7382
                     /locus_tag="OQM83_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1521401.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            7607..9262
                     /gene="opgD"
                     /locus_tag="OQM83_003208"
                     /gene_synonym="mdoD"
     CDS             7607..9262
                     /gene="opgD"
                     /locus_tag="OQM83_003208"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCS1521402.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            9402..9626
                     /locus_tag="OQM83_003209"
     CDS             9402..9626
                     /locus_tag="OQM83_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCS1521403.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            9689..10225
                     /gene="rimL"
                     /locus_tag="OQM83_003210"
     CDS             9689..10225
                     /gene="rimL"
                     /locus_tag="OQM83_003210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCS1521404.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
BASE COUNT         2645 a         2378 c         2630 g         2613 t
ORIGIN      
        1 agtggcgcgc gcagtagaag aaggggcgag agtggcgttg ggtggcaaag cggtagaggg
       61 gaaaggatat tattatccgc cgacattgct gctggatgtt cgccaggata tgtcgattat
      121 gcatgaggaa acctttggcc cggttctgcc ggtggtcgca tttgacacgc tggaagaggc
      181 tatctcaatg gctaatgaca gtgattacgg cctgacctca tcaatctata cccaaaatct
      241 gaacgtcgcg atgaaagcca ttaaagggct gaagtttggt gaaacttaca tcaaccgtga
      301 aaacttcgaa gctatgcaag gcttccacgc cggatggcgt aaatccggta ttggcggcgc
      361 agatggtaaa catggcctgc atgaatatct gcagacccag gtggtttatt tacagtcgta
      421 atgagtaaaa gaggcggagg ttttttcctc cgcctgtgcg cgtcagagtt tagcgaattt
      481 ttcgagggtg cgaataagct gcgtgacgaa gccatattcg ttatcgtacc aggcgaccgt
      541 tttcaccagt tgtaaatcgc ccacggcggt aatttccgtt tgcgtggcat caaacaccga
      601 accgaaatgg ctgccaatga tatcggaaga gactatttct tcatcggtat aaccaaatga
      661 ctcgttattg gtggttgctt gtttaagtgc gttattcacc tcttcggcag tcactttttt
      721 tccgagaatc gataccagtt cagtgaccga acctgttttc accggcacgc gttgcgcatg
      781 acctttcagt ttgccgctca gttccgggat caccagacca atggcttttg ccgcccccgt
      841 agtgtgggga atgatatttt ctgccgctgc gcgtgaagca cgtaaatctt taccacgtgg
      901 gccatccacc agtgactggg tgccagtata ggcatgaatg gtcgtcatcg tgccgacttc
      961 tatcccgaaa ctgtcatgca aggctttggc catcggcgca agacagttag tggtgcatga
     1021 cgccacggaa acaatggtgt cgttgccatc cagagtgtcg tcattgacgt tataaacgat
     1081 agttttcatt tcaccggcag gggcggaaat caacaccttc ttcgcaccag catcaagatg
     1141 tgcctgcgat ttctcggcgg aggtataaaa gccagtacat tcgacaatga tttctgcacc
     1201 tttcgctttc cacggaatat ttttagcctc tttttcggcg taaaccgcga tacttttccc
     1261 atcaacgata agtgaatctt ccgtaaaatc aacgctccag gggaatggtc cgtagtttga
     1321 atcatgtttc agcaggtagg cgagaatttt tggggaagtg agatcattaa tagcgacaac
     1381 gtctatgttg cttttgactt caagtaatcg acgcaacacc agtcgaccga tacgaccaaa
     1441 accgttaata ccaactttac tcatggtttt ctcctgtcag gaacattcgg atgaaaattg
     1501 atcctttcca ggcttagacc aggattgtgg gatgggcaat ctccattctt acagtgaaac
     1561 gtaacgtact gaaaacgggt gaacaatatt taatgaaatt ttgagaaaag cccgttatgt
     1621 taacggaaaa ttatgttaaa gcaggaaatg ttatggaaaa taaatactca aggttacaaa
     1681 tcagcattca ctggctggtc tttttgctgg ttatcgcagc gtattgcgca atggagtttc
     1741 gtggtttctt cccacgtagc gatcggccac tcatcaacat gattcatgtt tcctgcggca
     1801 tctcaattct cgtgctgatg gtcgttcgtc ttttgttaag gctgaaatac ccaaccccgc
     1861 cgattatacc taagccaaaa ccgatgatga cgggactggc gcatttggga catttggtga
     1921 tgtatctcct gtttattgcg ctgccagtga ttggtttggt gatgatgtat aaccggggca
     1981 acccgtggtt tgcgtttggt ttgacgatgc cttacgcttc agaggccaat ttcgaacggg
     2041 ttgatagctt aaagtcgtgg catgagacgc tggcgaatct gggatatttt gtcatcgggt
     2101 tgcacgctgc ggctgcactg gcacaccact atttctggaa ggacaacaca cttctacgca
     2161 tgatgccgcg taaacgttcc tgaaggatat ttcaagaaaa tgcctgtact aaaaccgacc
     2221 cgtggtacag gcgaagaata cgggtctaca tcggaagcgc ctattatatt tatttgtatg
     2281 acaaataaaa ccataatcct tgcccatacg tccatctggc ttatttttaa tcaattcacc
     2341 cgatctttga tctcatcaac ggtatcaaaa taaaccagcg tataacgttc aaatcgaaca
     2401 taagattcga taaaccatgg aggttatatg aaaaaactgg cacttatttt gtttatggga
     2461 acgcttgttt ccttttatgc cgatgccggg cgcaaaccct gttctggttc gaaagggggg
     2521 atctcacact gtacggcagg cggcaaattt gtctgtaatg atggttctat tagtgcatcg
     2581 aaaaaaacat gcactaactg aagtgtaaaa ggggtgccat gagaaaatga atctgctagt
     2641 cagatgcgcg gggaaaatcc ccgcgcttgc ccttacctgg acgtgcaggc catgagcgca
     2701 gcaacctcct tatcaccgtc ccggaaccgc agttcgtaga gcgtttgtcg ggtcaggagt
     2761 gtgaatgtca gaatcgtaat gcagataatg agcagacaca ctaccagagg gttgtgcttc
     2821 atagcctttc tccttgccgg atggcgggta agaggctaag atctgaattg ctaggttcat
     2881 tcgttggcct cggttgatag aaatatcggt cggggccttc gtctttctga ttcccggtta
     2941 gcctgaaaac agaaagtctc aggcacccgc aggcatccta tgaggtttcc ttagggacga
     3001 aaataatcac ttcacgaaat tgcgtgctgt tttccagaat ttttcgtcat tcgggttagc
     3061 cagtttagcc attcgttact ctcttcattc caatagcatt aattttctct gcaataattg
     3121 ttgtaaaaat gtgacgcaaa gaggtttttg gtcataagta attaccgtca agtgccgatg
     3181 actttctatc aggagtaaac ctggacgaga gacaacggta atgaatacaa ctccctcaca
     3241 gcgattaggt tttttgcatc acatcaggct ggttccgtta tttgcctgca ttcttggcgg
     3301 tatcttagtt ctattcgcat taagttctgc tctggctggc tatttcctct ggcaggccga
     3361 tcgcgatcag cgtgatgtta ctgcggagat tgagattcgg accgggttag cgaacagttc
     3421 agattttttg cgttcagccc ggatcaatat gattcaggcc ggggctgcga gttgtattgc
     3481 ggaaatggaa gcaatgaagc gaaatattgc gcaagccgaa tcggagatta aacagtcgca
     3541 gcaagggtat cgtgcttatc agaatcggcc ggtgaaaaca cctgctgatg aagccctcga
     3601 cactgaatta aatcaacgct ttcaggctta tatcacgggt atgcaaccta tgttgaaata
     3661 tgccaaaaat ggcatgtttg aagcgattat caatcatgaa agtgagcaga tccgaccgct
     3721 ggataatgct tataccgata ttttgaacaa agccgttaag atacgtagca ccagagccaa
     3781 ccaactggcg gaactggccc atcagcgcac ccgcttgggt gggatgttca tgattggcgc
     3841 gtttgtgctt gccctggtca tgacgctgat aacatttatg gtgctacgtc ggatcgtcat
     3901 tcgtccactg caacatgccg cacaacggat tgaaaaaatc gctagtggcg atctgacgat
     3961 gaaggatgaa ccggcgggtc gtaatgaaat cggtcgctta aatcgtcatt tacagcaaat
     4021 gcagcattca ctggggatga cagtagggac tgttcgacag ggtgcggaag agatttatcg
     4081 tggcaccagc gaaatttcag ctggcaatgc ggacctgtca tctcgcaccg aagaacaagc
     4141 ggcggctatc gaacaaactg ccgccagcat ggagcaactc actgcgacgg tgaaacagaa
     4201 tgcggataac gcgcatcatg ccagcaaact ggcgcaagag gcttctatta aagccagcga
     4261 tggcgggcag acggtttccg gtgtagtaaa aacgatgggc gctatctcca cgagttcgaa
     4321 gaaaatttct gagatcaccg ccgtcatcaa cagtattgct ttccagacga atattctggc
     4381 actgaatgct gccgttgaag ccgcgcgagc gggtgagcaa gggcgtggat ttgccgttgt
     4441 cgccagcgaa gtacggacac tcgcaagtcg cagcgctcag gcggcgaaag agattgaagg
     4501 cttgatcagt gaatcagtca ggttaattga cctggggtcg gatgaggtgg caacggccgg
     4561 gaaaaccatg agcactattg ttgatgccgt cgcgagtgtc acacatatca tgcaggaaat
     4621 cgccgccgcc tcggatgaac aaagtagagg cataacgcag gttagccagg cgatttctga
     4681 aatggataag gtgacgcaac agaatgcttc tctggtagaa gaggcctcag cggcggcggt
     4741 gtcccttgaa gaacaggcgg cacgattaac tgaggcggtg gatgtattcc gtctgcacaa
     4801 acattctgtg tcggcagaac ctcgcggagc gggtgaacca gttagtttcg ctacggtgtg
     4861 aaaatgttca aggagggatc gacagatccc ttcacctttc agaacggcat tgattttcga
     4921 atagcgttaa tcatcaactg gcaaccagaa gagaacgtcg catctacgcg ggtcagtatt
     4981 ccaatcggtt cgcctgcacc atgtcccgga acaggcaggg ccaccagcgt ggcatgacgc
     5041 aggtcgtctt ttacagcgcc agaagggaca aaccacacgt aatcgtattc aaccgtaagt
     5101 tgacgagata gcgaagcaga cagcgtttcg atacaacccg aaggaatttt acagccctgg
     5161 ctctgcacta atgcatctga atgctggcgt ggcgcagtgc cttctggtga tacaacgacc
     5221 ggccattcca gcacccggct tagcgttacg ttctcctgaa gtagcgggtg attagggcgg
     5281 acaaccagct tcagcgattc aagaaacagc agttcgtaat taagcccggt catcagttca
     5341 ggatctgaca tccgaccaat gccgatatcg atttccccgg tttttaaacc cgccagaatc
     5401 atagggttac tcattgtcgc aacttgcaag gtcgtctctt tttgttgctg atgaaactga
     5461 cctataaccg aaggtaatat ccccagtgcc gcagtaggta gtgcaccaac cctgacgaca
     5521 tcattattaa gaccttcttt acgatgaagc gactgtccgg cagtgttgat ggcgtcaaga
     5581 actctgactg catgcgttaa aaattgttcg ccgggtaagg taagttgcgc cccctgacga
     5641 ccacgctcaa acaagcgagc gcccgtcagc tgctccagtt cattcaatgt cttagagagc
     5701 gcaggttgac tcaaattaag ggtttcagcc gcgcgcccca aagttccttg ttgtgcgaca
     5761 gctacgaatg tatgaaggtg gcgcaaacgg atgcgctgac taaacagact atttttttcc
     5821 ataagcgatg ttaaaaacga agcggtgtcg ctgacaagtg aagttgtttg attatgataa
     5881 cttgattgca aaatattatt aacaattaaa gcaattatgt tacagcaaaa tggataatat
     5941 tgatgttttc gcggcgagat cacagtttgt aaattcttcc cgccagagtg aatgcggtta
     6001 cctacactcc agattactga ccactggagg cagacactat ggcgaacagc atcacggcgg
     6061 atgagattcg ggaacagttt tcgcaggcaa tgtcagccat gtaccagcaa gaagttccgc
     6121 aatatggcac gctgctggaa ctggtagctg atgtgaatct ggctgtgctg gaaaacaatc
     6181 ctcaactgca cgaaaaaatg gtaaatgcag acgagctggc gcgactgaat gttgaacgtc
     6241 atggggcgat tcgcgttggg actgcacaag agcttgctac tcttcggcgg atgtttgcca
     6301 ttatggggat gtacccggtg agctattacg atctctcgca ggcaggggtg ccggtacatt
     6361 cgacagcatt tcggcccatt gatgatgctt ctctggcgcg taatcccttc cgcgttttta
     6421 cctccttact ccgccttgag cttatcgaga acgaaatttt gcgccagaaa gcggcggaga
     6481 ttctgcgtca gcgcgatatc ttcaccccac gttgtcgaca actgttagag gaatatgagc
     6541 agcagggcgg ttttaacgaa acacaggcac aggagtttgt gcaggaagcc ctggaaacgt
     6601 ttcgctggca ccagtcagca acggtagatg aagaaaccta tcgcgccttg cacaacgaac
     6661 atcggttgat tgctgatgtg gtctgttttc ctggatgcca tatcaaccac ctgacgccac
     6721 gtacgctgga tattgaccgg gtgcagtcga tgatgcctga atgcggaatt gaacccaaaa
     6781 ttctcatcga ggggccgccg cgccgcgagg taccgatttt actacgccag accagcttta
     6841 aagcactgga agagacggtg ttgtttgcgg ggcagaaaca gggcacgcat accgcgcgct
     6901 ttggtgaaat tgagcagcgt ggcgtggcat taacgccgaa agggcgacaa ctgtatgatg
     6961 atcttctgcg taacgctgga accgggcagg ataatctcac tcaccaaatg catttacagg
     7021 aaaccttccg cacttttcct gacagtgagt ttttaatgcg tcagcaaggg ttggcatggt
     7081 tccggtaccg tctgacgcct tcgggtgagg cgcatcgtca ggcgattcat cctggagacg
     7141 atccacagcc cttaattgaa cgtggttggg tagtggcgca acccatcacc tatgaagatt
     7201 tcttgcccgt tagcgcggcg gggatcttcc agtcaaatct gggtaatgaa acgcagacac
     7261 gcagtcacgg taatgccagt cgcgaagcat ttgagcaggc gttgggttgt ccggttttgg
     7321 atgagttcca gctttaccag gaagcggaag aacgcagtaa acgtcgctgt ggtttgcttt
     7381 aaattctgac catccgcctt tgcaaaaatt tgcctgattt tacaaacgaa tcaggctcat
     7441 cccatcgaca taaaaaaaat gccgatttat gcatattctc tcagttcaac aattggatta
     7501 ttaataaata ttgtctagag tgagcggtca taaataagca ctttcttgcc gctgaaaacg
     7561 accagcgcgg gaccattcac aacaccagaa ggactcactt tcaggtatgg atcgtagacg
     7621 atttattaaa ggttcaatgg ctatggccgc cgtgtgcggt accagcggca ttgcttctct
     7681 tttttctcag gcggcattcg cggcagattc tgatattgcc gacgggcaaa cccagcgttt
     7741 tgacttctcc attctacagt caatggcgca cgacttagcg caaacagcgt ggcgtggtgc
     7801 gcctcgtccg ttacctgaca cgctggcgac aatgacgccg caggcttata acagtattca
     7861 atacgacgcc gaaaaatcgc tctggcataa cgttgagaac cgtcaactgg acgctcagtt
     7921 cttccatatg ggaatgggat tccgtcgccg cgttcgtatg ttttctgtag atccagcaac
     7981 acatctggcg cgtgaaattc actttcgccc ggagttgttc aaatacaacg atgcaggtgt
     8041 tgataccaaa caattagaag ggcaaagcga tctcggcttt gccggttttc gcgtgtttaa
     8101 agcccccgaa ctggcgcgcc gtgatgtagt atcatttctc ggcgcgagtt atttccgcgc
     8161 cgttgatgat acatatcaat acggtttgtc ggcccgcggc ctggcgatcg acacttacac
     8221 cgacagtaaa gaagagttcc ccgactttac cgccttctgg tttgatacgg taaaaccggg
     8281 ggcaactacc tttaccgttt atgcgttgct cgatagcgcc agtattactg gtgcctataa
     8341 gttcactatc cattgtgaga aaagtcaggt gattatggat gtggaaaatc acctgtatgc
     8401 gcgcaaagac attaaacagc tgggcattgc gccgatgacc agtatgttca gctgcggtac
     8461 taatgaacgt cggatgtgcg acaccattca tccgcaaatt catgactctg atcgtctgtc
     8521 catgtggcgg ggcaacggcg agtggatttg ccgtccgctg aacaatccgc aaaaattgca
     8581 gttcaatgct tacaccgaca acaacccgaa agggtttggt ttattgcaac tggatcgtga
     8641 cttctcccat tatcaggaca ttatgggctg gtataacaaa cgcccaagtt tgtgggtgga
     8701 accgcgtaac aagtggggta agggcaccat cggcctgatg gaaatcccaa caacgggcga
     8761 aacgctggat aacattgtct gcttctggca gccagaaaaa gctgtaaaag caggtgatga
     8821 gtttgcattc cagtatcgtc tgtactggag tgcgcaaccg cctgttcatt gcccattagc
     8881 gcgcgttatg gcgacgcgta ccggcatggg cggtttcccg gaaggttggg cgccaggtga
     8941 acactatccc gaaaaatggg cgcgtcgttt tgccgtcgat ttcgttggtg gtgatctgaa
     9001 agctgccgcg ccaaaaggca ttgagccggt gattacgctt tccagtgggg aagcgaagca
     9061 aatcgaaatt ctctatattg aacccatcga tggttatcgt attcagtttg actggtatcc
     9121 gacttcggac tctactgatc cggtcgatat gcggatgtat ctacgttgtc agggggacgc
     9181 tatcagtgaa acatggctgt atcagtattt cccgccagcg ccggataaac gtcagtatgt
     9241 tgacgaccgc gtgatgagtt aatcgttttt tcttcggcac cttcttcggg aggtgccgtc
     9301 tggttaaaca cgatcccgct cgcatttttc cctaagttaa atgagtaatc tgatggtgtg
     9361 tatttcagat acaccttgtc agccactaac agggagtgcg tatgtttcca gaataccgag
     9421 atttaatatc ccgtctgaaa aacgaaaatc ctcgctttat gtccttgttc gataaacaca
     9481 ataaacttga tcatgaaatt gccagaaagg aaggttccga cggtcgaggg tacaatgcgg
     9541 aagtggtccg catgaaaaaa caaaagctac agttaaaaga tgagatgctc aaaatcctgc
     9601 agcaggagag cgtcaaagag gtgtaaactt tcctaagccg ccagccaggc ggctttttta
     9661 acaacagcat ggattgactg gagataagat gactgaaacg ataaaagtaa gcgaatcact
     9721 tgaattacat gctgttgcgg aaagtcacgt cacacctctt tatcagttaa tctgtaaaaa
     9781 taaaacctgg ttacagcagt cgctaaactg gccgcagttc gttcagagtg aagaggacac
     9841 gcgaaaaacg gtgcagggta atgtgatgtt gcatcaacgc ggctatgcca aaatgttcat
     9901 gattttcaaa gaagatgaac ttatcggcgt tatctcgttt aatcgtattg aaccactgaa
     9961 taaaactgct gaaatcggct actggctgga cgaatctcat caggggcagg ggatcatttc
    10021 tcaggcgctg caggcattga ttcatcatta cgctcagtct ggtgaactta gacgcttcgt
    10081 gatcaaatgt cgggtggaca atccgcaaag caaccaggtc gctttgcgca atggttttat
    10141 ccttgaaggt tgcctgaaac aggctgagtt cctgaatgat gcctatgatg atgtgaactt
    10201 atacgcgcgt attatcgatt cataataacc ctgccagcgg cgttcgcgta atgcgctcct
    10261 cgccgt
//
LOCUS       DAJVQG010000138        10262 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJVQG010000138 DAJVQG010000000
VERSION     DAJVQG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.138"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="OQM83_003211"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="OQM83_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCS1521405.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="OQM83_003212"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="OQM83_003212"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCS1521406.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="OQM83_003213"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="OQM83_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HCS1521407.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="OQM83_003214"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="OQM83_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCS1521408.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="OQM83_003215"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="OQM83_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="OQM83_003216"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="OQM83_003216"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCS1521409.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="OQM83_003217"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="OQM83_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCS1521410.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="OQM83_003218"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="OQM83_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCS1521411.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="OQM83_003219"
     CDS             complement(9457..10149)
                     /locus_tag="OQM83_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCS1521412.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2494 a         2474 c         2719 g         2575 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ct
//
LOCUS       DAJVQG010000139        10257 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJVQG010000139 DAJVQG010000000
VERSION     DAJVQG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10257
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.139"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(43..1047)
                     /gene="cra"
                     /locus_tag="OQM83_003220"
     CDS             complement(43..1047)
                     /gene="cra"
                     /locus_tag="OQM83_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCS1521413.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1129..1215)
                     /locus_tag="OQM83_003221"
     CDS             complement(1129..1215)
                     /locus_tag="OQM83_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCS1521414.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1227..1718)
                     /gene="ilvN"
                     /locus_tag="OQM83_003222"
     CDS             complement(1227..1718)
                     /gene="ilvN"
                     /locus_tag="OQM83_003222"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCS1521415.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1721..3445)
                     /gene="ilvI"
                     /locus_tag="OQM83_003223"
     CDS             complement(1721..3445)
                     /gene="ilvI"
                     /locus_tag="OQM83_003223"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCS1521416.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3564..3608
                     /locus_tag="OQM83_003224"
     CDS             3564..3608
                     /locus_tag="OQM83_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521417.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3763..4707)
                     /gene="leuO"
                     /locus_tag="OQM83_003225"
     CDS             complement(3763..4707)
                     /gene="leuO"
                     /locus_tag="OQM83_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCS1521418.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5367..5453
                     /gene="leuL"
                     /locus_tag="OQM83_003226"
     CDS             5367..5453
                     /gene="leuL"
                     /locus_tag="OQM83_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCS1521419.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5546..7117
                     /gene="leuA"
                     /locus_tag="OQM83_003227"
     CDS             5546..7117
                     /gene="leuA"
                     /locus_tag="OQM83_003227"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCS1521420.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7117..8208
                     /gene="leuB"
                     /locus_tag="OQM83_003228"
     CDS             7117..8208
                     /gene="leuB"
                     /locus_tag="OQM83_003228"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCS1521421.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8211..9611
                     /gene="leuC"
                     /locus_tag="OQM83_003229"
     CDS             8211..9611
                     /gene="leuC"
                     /locus_tag="OQM83_003229"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCS1521422.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFNGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKTGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9622..10227
                     /gene="leuD"
                     /locus_tag="OQM83_003230"
     CDS             9622..10227
                     /gene="leuD"
                     /locus_tag="OQM83_003230"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCS1521423.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
BASE COUNT         2682 a         2695 c         2511 g         2369 t
ORIGIN      
        1 cgccagaggg aaattcacct ggcgcgtatt tttgttcgcg gcttagctac ggctgagcac
       61 accgcggcga tagagattac gtttaatgcg cgttaaacca ggttttggct tacgcggttc
      121 gtccaggctt gccaggacaa tctccagcac acgctctgcg acatcgcggt gacgttgagc
      181 cactgccagc accgggcact gtaagaagtc gagcagttcg ttatcgccaa aggtggcaat
      241 tgccaggtca gaaggcagtt taccatcgcg acgtagcgtg acatccatca ccccttgcaa
      301 caacgcaaac gacgttgtga acagcgcctg cggcatcgga tgcgtttcca gccatttttc
      361 gaataactgg gcagccacct cccgctcata gctgttggca tacaggaaat gcacttcgcg
      421 cggatcatct ttccaggcag tacggaaacc ttgttcacgc aggaagctga cagaaagctc
      481 cggcagcgcg ccaagataaa gcaccgtctc ggcgggaaac ttacgtaact cttccgccag
      541 catttcggca tcatcctgat cggcaccaac cacgctggtg aagtgttcac gatcgagggc
      601 gcggtccagc gcgacaatcg ggaacgggtc gttagcccag cgttgataaa aaggatgctc
      661 aggaggcaac gacgtcgaaa caataatggc atcgacctga cgctgtaaaa ggtgctcaat
      721 acagcgcatt tcgttgtctg gctgatcttc tgagcaggca attagcagtt gataaccccg
      781 ttgccgcgcc tggcgttcaa gatagttagc gatgcgggta tagctggtgt tctccagatc
      841 ggggatcaca agaccaatag aacgtgtgcg tccagcacga agcccagctg ccacggcgtt
      901 cgggtggtaa ttgtgctcac gcaccacagc catgactttt tcaacggttt tgtcgctcac
      961 acggtattgc ttcgctttgc cgttaataac atagcttgca gtggtccgcg acactcccgc
     1021 cagccgagcg atttcatcca gtttcacaat tgccccttgc gtaaaatgta aaaaccatag
     1081 ccattgtact ggtatgggtt aaattcatta acatctaagc gcataaaatt aactgcggca
     1141 actgctttta ttccaggttc ccactgattt cgcaaaaaaa gcccaacgtg acatgttggg
     1201 ctgcaaattg cgcattcaga tcattctcaa cgcattattt tatcgccgcg cgaaagtccg
     1261 accacaccag aacgagcaac ttccacaatt ttcgccacct cgcgaatcga tgctaaaaat
     1321 gcatcaagct tatcgctggt gcctgctaat tgaacggtat aaagcgaggg tgtgacatcg
     1381 ataatttgcc cacggaatat ttccgtatta cgtttcactt cgtcacgccc gtaaccgctg
     1441 gcctgaattt tcaccagcat gatttcccgc tcaacatgcg cgccctgccc caactcactc
     1501 acgcgcaaga catcgaccag cttgtgtaat tgcttttcga tctgctcaag tactttttca
     1561 tcgcccacgg tctggatggt catacgcgat aatgtcggat catcggttga cgcaacggtc
     1621 aggctttcaa tgttgtagcc acgctgggaa aaaaggccaa tcacgcggga taacgcgcct
     1681 gattcatttt cgagtaagac tgataatatc cggcgcataa tcaggttctc tccgttttgc
     1741 ttaaccacat ttcatccatt ccgcccccgc gaatctgcat cgggtagacg tgctcgctgc
     1801 catcgacggt aacatcaaca aacaccaggc gattattgtg cacctgttcc agcgcctcgc
     1861 taagtttgct ttccagctca tgcggatgag aaatctggat cccgacgtga ccataggctt
     1921 ccgccagacg gacgaaatcg ggtagcgatt gcatataaga ttgtgaatga cggccggaat
     1981 agatcatgtc ctgccactgc ttcaccatcc ccagatagcg gttattgaga ttcaccacca
     2041 gtacgggcaa ctcgtattgc aacgcggtag acagttcctg gatgttcatc tgaatactgc
     2101 cgtcgccagt gacgcaaacc acggtttctt ctggcaacgc cattttgacg cccagtgccg
     2161 caggtaaacc aaaacccatc gtgccgaggc caccggaatt gatccagcga cgcggtttgt
     2221 cgaatggata ataaagtgca gcaaacatct ggtgctgccc gacatcggac gtcacgtaag
     2281 cgtctccctt cgtcaaccgc caaagagtct cgatcaccgc ctgcggttta atcttttcac
     2341 tgtgagtgtc atatttcagg cactgacgag cgcgccactg ttcaatttgc tgccaccagt
     2401 cgcggatctc atccagtggt tgatgggcgg attcttgcga caagagttca agcatttgtt
     2461 cgaggacctg gcgagcatcc cccacaatcg ggatatccgc agtcacggtt ttagaaatgg
     2521 aagtaggatc aatatcgata tgcagaacag tggcatttgg gcagtacttt gccagattgt
     2581 tcgtcgttcg gtcatcaaat cgtaccccga cggcgaaaat cacatccgcg ttatgcatcg
     2641 tcatattggc ttcgtaggta ccgtgcattc ccagcatgcc cagtgcctga cgatgcgttg
     2701 ccggaaacgc tcccagcccc atcaatgagg aaacaacggg cagattcaac gcctccaccg
     2761 tttctttcaa ctgctgatgg cagcccgccg tgattgcccc accgcctacg tagacaaccg
     2821 gtttttttgc cgctaccagc gtttgcagag cgcgcttaat ttgcccttta tgtccggtag
     2881 tagtgggatt gtaagaacgc atactgaccg actccggcca gacatagggt aatttgttcg
     2941 ccggattaag aatatctttc ggtaaatcaa cgactactgg tccaggacga ccacttgccg
     3001 ccagccagaa agcctttttc agcacctgcg gaatgtcttc cgtttgctta accagaaaac
     3061 tgtgtttaac cactggtcgc gaaatcccca ccatgtcgca ctcctgaaag gcatcgtaac
     3121 ctatcaacga ggtcgctacc tgcccggaaa ggacaactaa tggaatggaa tccatataag
     3181 cggtggcgat gccagtaatc gcattggtcg cccctggacc cgacgttacc agcacgacgc
     3241 cgacttcccc ggtcgcgcgc gccagaccat cggccatatg cactgccgcc tgctcatgac
     3301 gaactaaaac atgatcgata ccacccacgg tatgcaatgc atcgtaaata tcaaggactg
     3361 cgcctccggg ataaccgaat acttgtttaa cgccctgatc gataagcgat cggacgacca
     3421 tctcggctcc agacaacatc tccatggcct gcctcactgt ttgacggaaa aaatgtgtaa
     3481 aacacattaa ccgtccgatc ttgcttaagc aattggcagc cagagacata aacgcagggt
     3541 aataagaata aacaggagga aaggtgaaaa gaaacgataa aatcctccat tacgaaggat
     3601 taaattgata ataaatcggc tgaatcccac aacttacagc aatttctcta tttttttaat
     3661 taatcctaaa atagagagcc cacacattca gaataaaatt ctacattcaa taaaaaatac
     3721 agaataaacc agacattcat gtctgaccta ttctgcattc agttagcgtt tgcaaattga
     3781 gactaattgc tcttccatcc actgatggcc tttatcgcgc ccggcagctt catgccagga
     3841 gagataacag gttctgctat tttgttttaa cggcagcggt aatacctgta attctaagga
     3901 ttcagcgaac tcttcagcca gccaacgcgg cgcaatagcg accaaatgcg tttgcgacac
     3961 cacgctaagt acgctcatca ttgccatgcc ctgatacgcg atactggctt gcttatctac
     4021 cgtgtcatac caaggttgac taaatgacgc gaaacgatcg agcgaaaccg ccgcatgttg
     4081 ttcgttataa acatcatgtt tcagtaacgg gcccttaatt gttggatgat ttttgctggc
     4141 taccagcacc atttcatctt taaataatgg cacgctggta aattcaggac gatggaagtc
     4201 ttcataacta atcacaaact ccgtttcctg ataacgcagc tgatgttcag tgttctgatt
     4261 taatgaagac ttgaacataa catgtatatt tggcgcaatc tgctcaatgt gattataaat
     4321 ctgcgaggtc agaatgctgt ctaacgggct gcaaacacaa agatgaaata cacgttcact
     4381 gctcgcgggt tcaaaacctg aaccaggcaa ttcattttgt actagttgca atgcctgacg
     4441 aactgagcca aaaagttgaa atgcgcgagc agtcggttga ataccacggc cataacgaac
     4501 aaaaagctcg tcattaaaca tcaccttcag gcgtgcaaca gcgttactga ccgcaggttg
     4561 cgacataccc agaacatgag cggcacgggt aatgttttgc tcctgcatca cggcatcgaa
     4621 aacggttaat aagttgagat cgaccatgcg tagctgtggc ttgcttaact ccgccgtctc
     4681 tgaatgatct gtttgtacct ctggcatact taactccact gtcacactta actccctttc
     4741 ccttattgga atgcagagaa ataatcctga agaatatgat ttatcatgca tataaaataa
     4801 gaaaaagcaa aatgagtaaa attcggaaaa cataaagata cgggttctga taaaaacaga
     4861 atccattgcc gtggaatgag tcatttacca cagcataaga atccataatg tttttgttgc
     4921 gaaaacaatc taatcataac taccgcgaat actcaatcat ctacaaaatg gattaaatgt
     4981 gcttatttaa taattaattt atatatttaa tgcattaatt cttaacatta attgatcaat
     5041 aatattcacc aaatcaatat caaaaaaaat cgcaaaacat ataattcaat acaaatcatc
     5101 aggataggtt ttgcaacgcg tgcattttgt cccctttttc ctcgttgatt agatgcaaaa
     5161 atttatgctg aaatatgtca accgatgaaa agcgtcggta gttaagcaga aattaatatc
     5221 gcttacttta accaccgcag cacaattagc taattttacg gatgcagaac tcacgctggc
     5281 gggacgtttt tattgcgtca gggttgacat ccgtttttgt atccagtaac tctaaaagca
     5341 tatcgcattc atctggagct gatttaatga ctcacatcgt tcgctttatc ggtctactac
     5401 tactaaacgc atcttctttg cgcggtagac gagtgagcgg catccagcat taagccagca
     5461 cgcagtcaaa caaaaaaccc gcgccattgc gcgggttttt ttatgcccga agcgaggcgc
     5521 tctaaaagag acaaggacct aaaccatgag ccagcaagtc attattttcg ataccacatt
     5581 gcgcgacggt gaacaggcgt tacaggcaag cttgagtgtg aaagaaaaac tgcaaattgc
     5641 gctggccctt gagcgtatgg gtgttgacgt gatggaagtc ggtttccccg tctcttcgcc
     5701 gggcgatttt gaatcggtgc aaaccatcgc ccgccaggtt aaaaacagcc gcgtatgtgc
     5761 gttagctcgc tgcgtggaaa aagatatcga cgtggcggcc gaatccctga aagtcgccga
     5821 agccttccgt attcatacct ttattgctac ctccccaatg cacatcgcca ccaagctgcg
     5881 cagcaccctg gacgaggtga tcgaacgcgc tatctatatg gtgaaacgcg cccgtaatta
     5941 caccgatgat gttgaatttt cttgcgaaga tgccgggcgt acacccattg ccgatctggc
     6001 gcgagtggtc gaagcggcga ttaatgccgg tgccaccacc atcaacattc cggacaccgt
     6061 gggctacacc atgccgtttg agttcgccgg aatcatcagc ggcctgtatg aacgcgtacc
     6121 taacatcgac aaagccatta tttccgtaca tacccacgac gatttaggcc tggcggtcgg
     6181 caactcactg gcggcggtac atgccggtgc acgccaggtg gaaggtgcaa tgaacgggat
     6241 cggcgagcgt gccggtaact gttcgctgga agaagtcatc atggcgatta aagttcgtaa
     6301 ggatattctc aacgtccaca ccgccattaa tcaccaggag atatggcgca ccagccagtt
     6361 agttagccag atttgtaata tgccgatccc ggcaaacaaa gccattgttg gcagcagcgc
     6421 attcgctcac tcctccggta tccaccagga tggcgtgctg aaaaaccgcg aaaactacga
     6481 aatcatgaca ccagaatcta ttggtctgaa ccaaatccag ctgaatctga cctctcgttc
     6541 ggggcgtgcg gcggtgaaac atcgcatgga tgagatgggg tataaagaaa gtgaatataa
     6601 tttagacaat ttgtacgatg ctttcctgaa gctggcggac aaaaaaggtc aggtgtttga
     6661 ttacgatctg gaggcgctgg ccttcatcgg taagcagcaa gaagagccgg agcatttccg
     6721 tctggattac ttcagcgtgc agtctggctc taacgatatc gccaccgccg ccgtcaaact
     6781 ggcctgtggc gaagaagtca aagcagaagc cgccaacggt aacggtccgg tcgatgccgt
     6841 ctatcaggca attaaccgca tcactgacta taacgtcgaa ctggtgaaat acagcctgac
     6901 cgccaaaggt cacggtaaag atgcgctggg tcaggtggat attgtcgcca actacaacgg
     6961 tcgccgcttc cacggcgtcg gcctggccac cgatattgtc gagtcctccg ccaaagccat
     7021 ggtgcacgta ctgaacaata tctggcgtgc cgcagaagtc gaaaaagagt tgcaacgcaa
     7081 agctcaacac aacgaaaaca acaaggaaac cgtgtgatgt cgaagaatta ccatattgcc
     7141 gtattgccgg gggacggtat tggtccggaa gtgatgaccc aggcgctgaa agtgctgaat
     7201 gccgtgcgca accgctttgc gatgcgcatc accaccagcc attacgatgt aggcggcgca
     7261 gccattgata accacgggca accactgccg cctgcgacgg ttgaaggttg tgagcaagcc
     7321 gatgccgtgc tgtttggctc ggtaggcggc ccgaagtggg aacatttacc accagaccag
     7381 caaccagaac gcggcgcgct gctgcctctg cgtaagcgct tcaaattatt cagcaacctg
     7441 cgcccggcaa aactgtatca ggggctggaa gcattctgtc cgctgcgtgc agacattgcc
     7501 gctaacggct tcgacatcct gtgcgtgcgc gaactgaccg gcggcatcta tttcggtcag
     7561 ccaaaaggcc gcgaaggtag cggacaatat gaaaaagcgt ttgataccga ggtgtatcac
     7621 cgttttgaga tcgagcgtat tgcccgcatc gcgtttgaat ctgctcgtaa acgtcgccac
     7681 aaagtcacct caatcgacaa agctaacgtg ctgcaatcct ctattttatg gcgtgaaatc
     7741 gtcaacgaga tcgccacgga atacccggat gtcgaactgg cgcatatgta catcgacaac
     7801 gccaccatgc agctgattaa agatccatca cagtttgacg ttctgctgtg ctccaacctg
     7861 tttggcgaca ttctgtctga cgagtgcgca atgatcactg gctcgatggg gatgttgcct
     7921 tccgccagcc tgaacgagca aggttttggt ctgtatgaac cggcaggcgg ctcagcacca
     7981 gatatcgcag gcaaaaacat cgccaacccg attgcgcaaa ttctgtcgct ggcactgctg
     8041 ctgcgctaca gcctggatgc cgatgatgcg gcttgcgcca ttgaacgcgc cattaaccgc
     8101 gcattagaag aaggcattcg caccggggat ttagcccgtg gcgctgccgc cgttagtacc
     8161 gatgaaatgg gcgatatcat tgcccgctat gtggcagaag gggtgtaatc atggctaaaa
     8221 cgttatacga aaaattattc gacgctcacg tagtgtacga agccgaaaac gaaaccccgc
     8281 tgttatatat cgaccgccac ctggtgcatg aagtgacctc accgcaggcg ttcaatggtc
     8341 tgcgcgccca cggtcgcccg gtacgtcagc cgggcaaaac cttcgccact atggatcaca
     8401 acgtctctac ccagacgaaa gacattaatg cctgtggtga aatggcgcgc atccagatgc
     8461 aggagctgat taaaaactgc aaagaatttg gcgtcgaact gtatgacctg aatcacccgt
     8521 atcaggggat cgtccacgta atggggccgg aacagggcgt caccttgccg gggatgacca
     8581 ttgtctgcgg cgactcgcat accgccaccc acggcgcgtt tggcgcactg gcctttggta
     8641 tcggcacttc cgaagttgaa cacgtactgg caacgcaaac cctgaaacag ggccgcgcaa
     8701 aaaccatgaa aattgaagtc cagggcaaag ccgcgccggg cattaccgca aaagatatcg
     8761 tgctggcaat tatcggtaaa accggtagcg caggcggcac cgggcatgtg gtggagtttt
     8821 gcggcgaagc aatccgtgat ttaagcatgg aaggtcgtat gaccctgtgc aatatggcaa
     8881 tcgaaatggg cgcaaaaacc ggtctggttg caccggacga aaccaccttt aactatgtca
     8941 aaggccgttt gcatgcgccg aaaggcaaag atttcgacga cgccgttgcc tactggaaaa
     9001 ccctgcaaac cgacgaaggc gcaactttcg ataccgttgt cactctgcaa gcagaagaaa
     9061 tttcaccgca ggtcacctgg ggcaccaatc caggccaggt gatttccgtg aacgacaata
     9121 ttcccgatcc ggcttcgttt gccgatccgg ttaaacgcgc gtcggcagaa aaagcgctgg
     9181 cctatatggg gctgaaaccg ggcattccgc tgaccgaagt ggctatcgac aaagtgttta
     9241 tcggttcctg taccaactcg cgcattgaag atttacgcgc ggcagcggag atcgccaaag
     9301 ggcgaaaagt cgcgccaggc gtgcaggcac tggtggttcc cggctctggc ccggtaaaag
     9361 cccaggcgga agcggaaggt ctggataaaa tctttattga agccggtttt gaatggcgct
     9421 tgcctggctg ctcaatgtgt ctggcgatga acaacgaccg tctgaatccg ggcgaacgtt
     9481 gtgcatccac cagcaaccgt aactttgaag gccgccaggg gcgcggcggg cgcacgcatc
     9541 tggtcagccc ggcaatggct gccgctgctg ctgtgaccgg acatttcgcc gacattcgca
     9601 acattaaata aggagcacac catggcagag aaatttatca aacacacagg cctggtggtt
     9661 ccgctggatg ccgccaatgt cgataccgat gcaatcatcc cgaaacagtt tttgcagaaa
     9721 gtgacccgta cgggttttgg cgcgcatctg tttaacgact ggcgttttct cgatgaaaaa
     9781 ggccaacagc caaacccgga cttcgtgctg aacttcccgc agtatcaggg cgcttccatt
     9841 ttgctggcac gagaaaactt cggctgtggc tcctcgcgtg agcacgcgcc ctgggcattg
     9901 accgactacg gctttaaagt ggtgattgcg ccgagttttg ctgacatctt ctacggcaat
     9961 agctttaaca accagctgct gccggtgaaa ttaagcgatg cagaagtgga cgaactgttt
    10021 gcgctggtga aagccaatcc ggggatccat ttcgacgtgg atctggaagc gcaagaggtg
    10081 aaagcgggag agaaaaccta tcgctttacc atcgatgcct tccgccgcca ctgcatgatg
    10141 aacggtctgg acagtattgg gcttaccttg cagcacgacg acgccattgc cgcttatgaa
    10201 gcaaaacaac ctgcgtttat gaattaatcc ccttgcccgg tcaaatgacc gggcttt
//
LOCUS       DAJVQG010000140        10008 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJVQG010000140 DAJVQG010000000
VERSION     DAJVQG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10008
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.140"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..784)
                     /locus_tag="OQM83_003231"
     CDS             complement(<1..784)
                     /locus_tag="OQM83_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521424.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="OQM83_003232"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="OQM83_003232"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCS1521425.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="OQM83_003233"
     CDS             1860..2747
                     /locus_tag="OQM83_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCS1521426.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="OQM83_003234"
     CDS             2780..3790
                     /locus_tag="OQM83_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCS1521427.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="OQM83_003235"
     CDS             3860..5293
                     /locus_tag="OQM83_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1521428.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="OQM83_003236"
     CDS             5392..6741
                     /locus_tag="OQM83_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCS1521429.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="OQM83_003237"
     CDS             6731..7618
                     /locus_tag="OQM83_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCS1521430.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRIFPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7759..8358
                     /gene="yihX"
                     /locus_tag="OQM83_003238"
     CDS             7759..8358
                     /gene="yihX"
                     /locus_tag="OQM83_003238"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCS1521431.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8352..9224
                     /locus_tag="OQM83_003239"
     CDS             8352..9224
                     /locus_tag="OQM83_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCS1521432.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9221..9658
                     /gene="dtd"
                     /locus_tag="OQM83_003240"
     CDS             9221..9658
                     /gene="dtd"
                     /locus_tag="OQM83_003240"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCS1521433.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9703..>10008
                     /locus_tag="OQM83_003241"
     CDS             9703..>10008
                     /locus_tag="OQM83_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1521434.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     "
BASE COUNT         2307 a         2356 c         2656 g         2689 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagt attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgccgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgctgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccgag taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatc gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacaggcg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tttcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc
     7801 gactttaacc gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt
     7861 aagaagagtt ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac
     7921 gaagcgttcg cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc
     7981 tctcacggct ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat
     8041 aaactgcgtg agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc
     8101 accttctggc cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg
     8161 caagatctgg ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa
     8221 ggtttttcac ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagcc
     8281 aatcagctgg gcattaccag cattctggtg aaagataaaa ccaccatccc ggactatttc
     8341 gcgaaggtgt tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat
     8401 gggcctggct aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag
     8461 gtaaccttgc ctatgtgtct ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc
     8521 ttttcgccgc ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg
     8581 ccaactttct gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcca
     8641 attccaacaa gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga
     8701 tgtactccat cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa
     8761 tttactcgtt tgccgtgtac tggatgatct taacgctggg gccgctgctg gcaggggcca
     8821 gtctggcgat cagttcctat ttgttctccc tgcgctgggc gagcgatctc aatactgtca
     8881 tcgataacgt gctgcgtatt tttccgctgc tgttgtcgtg gatctccttc tggttgctgt
     8941 acagcattgt tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg
     9001 ctgcactcct gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt
     9061 catatcagct catttacggc gtgctggcgg tgatccccat tctcttcgtt tgggtctact
     9121 ggacatggtg tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca
     9181 aactaaaaca agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg
     9241 cgtaacccgt gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact
     9301 tttggtgtta ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga
     9361 gcgtgtgctc ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca
     9421 acaggcgggc ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg
     9481 ggggatgcgc ccaagtttct ccaaaggcgc atcaccggat cgcgcagagg cgttatatga
     9541 ctatttcgtc gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc
     9601 cgatatgcag gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc
     9661 ctgcttccag ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcgggtt
     9721 ccacaaacag aagaagagtt agagcgttac tatcagtttc gctgggaaat gttgcgtaag
     9781 cccctgcatc aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag
     9841 atggtcgtcg acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac
     9901 aatgaagcgt ccattcgctt tatggccgtt cacccggacg tgcaggacaa agggttagga
     9961 acgctgatgg cgatgaccct ggagtcggtg gcgcgtcagg aaggcgtt
//
LOCUS       DAJVQG010000141         9819 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJVQG010000141 DAJVQG010000000
VERSION     DAJVQG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9819
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.141"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(37..1032)
                     /gene="gapA"
                     /locus_tag="OQM83_003242"
     CDS             complement(37..1032)
                     /gene="gapA"
                     /locus_tag="OQM83_003242"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCS1521435.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1374..1787
                     /gene="msrB"
                     /locus_tag="OQM83_003243"
     CDS             1374..1787
                     /gene="msrB"
                     /locus_tag="OQM83_003243"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCS1521436.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1829..2101
                     /locus_tag="OQM83_003244"
     CDS             1829..2101
                     /locus_tag="OQM83_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCS1521437.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2262..2903)
                     /gene="pncA"
                     /locus_tag="OQM83_003245"
     CDS             complement(2262..2903)
                     /gene="pncA"
                     /locus_tag="OQM83_003245"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCS1521438.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2914..3930)
                     /gene="ansA"
                     /locus_tag="OQM83_003246"
     CDS             complement(2914..3930)
                     /gene="ansA"
                     /locus_tag="OQM83_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCS1521439.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4097..5953)
                     /gene="sppA"
                     /locus_tag="OQM83_003247"
     CDS             complement(4097..5953)
                     /gene="sppA"
                     /locus_tag="OQM83_003247"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCS1521440.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6114..6665
                     /locus_tag="OQM83_003248"
     CDS             6114..6665
                     /locus_tag="OQM83_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCS1521441.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6782..7825
                     /gene="selD"
                     /locus_tag="OQM83_003249"
     CDS             6782..7825
                     /gene="selD"
                     /locus_tag="OQM83_003249"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCS1521442.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDATEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7830..9791
                     /gene="topB"
                     /locus_tag="OQM83_003250"
     CDS             7830..9791
                     /gene="topB"
                     /locus_tag="OQM83_003250"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCS1521443.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIIPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         2434 a         2450 c         2617 g         2318 t
ORIGIN      
        1 gaagtcgctc tttttagatc acagtttcat ctcaacttat ttggagatgt gagcgatcag
       61 gtccagaact ttgttggagt aaccggtttc gttgtcgtac caggatacca gtttcacgaa
      121 gttgtcgttc agagcgatac cagctttagc atcgaacacg gaagtgcaaa cttcgccgtt
      181 gaaatcggta gatactacgt catcttcggt gtagcccaga acgcctttca tttcgccttc
      241 agcagcggct ttaacggcag ctttgatctg ctcgtaagtt gcagcttttt ccagacgaac
      301 ggtcaggtca actacggata cgttcggggt cggaacgcgg aacgccatac cagtcagttt
      361 gccattcagt tctggcagta ctttacctac agctttagca gcaccggtag aggacgggat
      421 gatgttctgg gaagcgccgc ggccgccgcg ccagtctttg tgagacgggc catcaacggt
      481 tttctgagta gcggtagtag cgtgaacggt ggtcatcaga ccttcgatga tgccgaagtt
      541 atcgttgata actttagcca gcggagccag gcagttggtg gtgcaggaag cgttggaaac
      601 gatgtcctgg ccagcatatt tgtcgaagtt agcgccttta acgaacatcg gagtgttgtc
      661 tttagacgga ccagtcataa ccactttctt cgcaccagcg gtgatgtgtt tacgagcagt
      721 ttcgtcagtc aggaacagac cagttgcttc agcgacaacg tcaacaccaa cttcgtccca
      781 tttcaggtta gccggatcac gttcagcggt aacacggatt tttttaccgt taacgatcag
      841 atgaccgtct ttcacttcaa cggtaccgtc gaaacggccg tgagtggagt catatttcag
      901 catgtatgcc atgtaatcag cgtctaacag gtcgttgatt gcaacgatct cgatgtcaga
      961 acgtttctga gcagcacgga aaacaatgcg accgatacgg ccaaaaccgt tgatacctac
     1021 tttgatagtc atatattcca ccagctattt gttagtgaat aaaaggttgc ctgtaaaatt
     1081 acaaaaacct tacgcagcgt caagcggaat cgtgtcaatc attgcgacaa atcaatcctg
     1141 tgcctaagca ttacgcgact gactcgcctc actcttcctt tgggcttgaa accacatggg
     1201 gtcggcgccc cgaattttaa agggcaatta cgataaaaat gtgattttca tcacgattta
     1261 ggtgcagcca tttcgtcgag atcaagttta ggacaaaatt ttgccagttg gtgttaatgt
     1321 tttgttagaa tcagtcacgt aatgtgagca cgattaaagt gagatgtgag caaatggcta
     1381 ataaaccttc ggcagaagaa ctgaaaaaaa atttgtccga gatgcagttt tacgtgacgc
     1441 agaatcatgg gacagagccg ccatttacgg gtcgtttact gcataacaag cgtgacggcg
     1501 tatatcactg tttgatctgc gatgccccgc tgtttcattc ccaaaccaag tatgattccg
     1561 gctgtggttg gcccagtttc tacgaaccgg tgagtgaaga atccattcgt tatatcaaag
     1621 acttgtcaca tggaatgcag cgcatagaaa ttcgttgcgg taactgtgat gcccatctgg
     1681 ggcatgtctt ccctgacggg ccgcagccaa cgggcgaacg ttattgtgtt aactctgctt
     1741 ctttacgctt cactgatggc gaaaacggcg aagaaatcaa cggttgaaaa aacgattcag
     1801 caacttattc cacaggagcg gaaaaattat gaatcttgat gacattatca acagcatgac
     1861 gcctgaggta taccagcgtt tgtcgaccgc cgttgaactg gggaaatggc ccgatggcgt
     1921 tgcgttgacc gaggaacaaa aagcgaactg cctgcaactg gtgatgctgt ggcaagctcg
     1981 ccataatacc gaagcacagc atatgacaat tgacacaaat ggtcagatgg tcatgaagag
     2041 caaacagcag ttaaaagaag attttggtat ctcagcaaag cctattgcta tgtttaagtg
     2101 aatgagttat ctagtattta agcctgttat taagggaggg caaatatact tgttaggacg
     2161 tcgtgtttta gctgcaagta ggttaaatgc aacactgaca agtattatct ggcctacaat
     2221 gtcattgagg aaatgtaggc cagagatgaa ttcaactaat attacccctg tgtctcttcc
     2281 cagtctgcca gcgtatatag cgttgcccca gctgctgaca tctccataaa cgcgtgcgca
     2341 ctgtcctggg gctggatatt cacgccacga caaccatcgg taatcacatt taccttataa
     2401 cctaactgta acgcgtccag cacggtaaac ttcacgcaat agtcagtagc caggcccata
     2461 acgatcaatt catcgatttc atgatcgcgt aaccagtcat cgagcgaggt tttctgccga
     2521 cggccgttat caaaaaaggc actgtaactg tcaactaaag gattttcgcc tttatggaac
     2581 accgctgcga tcgctttttg gttcagtaac ggatgtaatt gtgcgccttc actgttctgc
     2641 acacagtgat ctggccagaa ggtttgtggc aaaccgtcga gttggcctgg cgtataaggc
     2701 tctacaccgt gctgactggc aaaactgccg tgattcgccg ggtgccagtc ctgactggcg
     2761 ataaccgctt caccgcgcga ctggcaccag tcaatcaggc ggttagcgac atccaccgta
     2821 ctgtcacctt ccggcacggc gagcgcgcca ccagcacaga aatcattttg taaatcgacc
     2881 agtaacaggg cgcgaggggg cattacagtc tccttaatca tccggcgtca gttcgccgcg
     2941 caggttttgg ctcatggcct tgcgaatggt ttcagtatcc agttcctggc tcagcaggta
     3001 atgcagtttg gttagcgtgg cttctacagt catatctgca ccgccaataa cgccggcatg
     3061 ggcgagggcg ttaccggtgg cataaccacc catattcact ttaccggaca tacattgtgt
     3121 caggttgacc accacaatac cgcgatcgct ggcttcttgt aattcctgca ggaaggcttt
     3181 gttttgtggc gcattaccca cgccatagga gcgcagaatc aatgctttca ccggttggcg
     3241 cagaaaattg cgcacgacgt cagcagaaat ccctggataa atcgtcacta cgccaattgg
     3301 ttgtggcgtg attggatgaa cgattaattc cccttcaccg tgcggggcgg gtggcgtatt
     3361 caaacgacga atatggatac cagcttccag taacggagga aggtttggag aggcaaacgc
     3421 atcaaaacca tcggcatggg ctttggtagt gcggttgccg cgatacaatc ggttattgaa
     3481 aaagagcgtt acttcgttga tcggataatt cgccgcaacg tacaacgcat tcagcagatt
     3541 aatttgtccg tcagagcgta actcagccag cgggatttgt gaccctgtca caatgaccgg
     3601 tttaccgaga ttctcgagca tgaacgacag cgcagaggca gtatacgcca tcgtgtcggt
     3661 gccgtgcagg atgacaaaac catcatagtc gtcatagtgc gctttaatat cttcagcaat
     3721 atgctgccag tcttctggcg tcatatcaga agaatccatc agcggcgtat attcatgaat
     3781 ggtgaaatct ggcatctccg ggcgatggaa ttccggcatc agcgccagtt ggcgttgtag
     3841 atgacctgac accggaatat aaccctgctc ggaacgctgc atcccgatgg tcccgcccgt
     3901 gtaggcgacg taaattgatt tcttttgcat gatattgata tgaatgatat cgaaagagca
     3961 aaagtatata cgtgaggcag gaggagcgca cctgatacta tgcggataag gaaatatggg
     4021 gcgagacagc gggaagagga ttcgtcgacg taaaaaataa tgtggccgac gatctgtcgg
     4081 ccactcagta caagacttaa cgcacgttgg cgcaggtcag gcaaaacgca taacggtttt
     4141 gtgggtcgtt aaacgcggcc agcttgtcgc tttcactttt aacggtagag gctaccgagg
     4201 caagcggtgc aggtaacatg gcctggaacg catctggcaa cattgcccgg acagaaccag
     4261 acatgttgtc catcactttg tcgaagaagg tcggttcatc aacgtagtat tccagatgcc
     4321 actgtttcac ttttgccagc tctgctgctt tggcgaccgc atcatcgaaa tccccgagac
     4381 tatcgaccag cccgttagct tttgcatcct gaccggtcca gacgtggccc tgggcgattt
     4441 tatcaatctg ctccggcgtc gaatgacgcg catcagcaac cagcgtgata aagcgtttat
     4501 agccattctc aatgcttaat tgcatcatct gctgcgcttc cggcggcagt gccctggtga
     4561 tagaaacatc cgccagcggt gaagttgaga caccatcagt atgaacacca atcgaatcca
     4621 gactattttc tacggtggtg atcacgccga agataccgat agaaccggtc agggtgctgg
     4681 ggttagccac aatgtaatta gctggcgtgg aaatccagta accaccagat gccgccatgc
     4741 cgcccatcga tacaaccaca ggcttacccg ctgcccgggc tgctgccagt tcagcgcgaa
     4801 tcacttcaga cgcggtaacg ctgccgcctg ggctattaac acgcaggaca atcgctttca
     4861 ctttcgggtc aaggcgagcg tcgcggattt gtgccgcagt ggtatcaccg ccaacattcc
     4921 cctgagtttc ctcgccatcc ataattgcgc cattagcgaa gacgacaccg atgctgtcac
     4981 cggtatctgc cggcgttttc aatgcgtaat cgtaataact gatggcgcga taatttttat
     5041 cagtcttact ccagccgaac tctttggtta gagctttttc gatttcggca ctcgatgcca
     5101 gtgcatcgac cagcttgttt tccagtgcat atttcgcggt atcgccaccg gttttggtta
     5161 aaccctcaag caacccttgc gcgccaggga atacctgctg agcagggatc tgccggttag
     5221 cggcaacagt attcagatag ttttgccaca gctcaccaat ccagcggctg tcagcttcgc
     5281 gggctgccgg tgacatgtca tcacgaataa acggttcaac ggcagattta tacgtaccca
     5341 cgcggaacac atgggtggaa actttcagct tatccagcaa cgatttgtag tacagaccgt
     5401 tggtagcaaa accgtgcaaa tccaccacgc cttgcggtga cagccaaatt ttattggcga
     5461 aactggcgag ataatattgc ccctggctgt agttctcgcc aacggcataa accggtttcc
     5521 cgctgtcacg aaactctttc agggctttgc cgatgtactg catagacggc tggtcgccgc
     5581 ctgcgaagtt tttcagatcc atcacgatac cggtgatatt gcggtcgtcc ttcgcctggc
     5641 gaatggtgtt gacgatatca aacagtgagt tttcctgcag acgatcggaa ctggcaccaa
     5701 gcagctggcg gcttaattta ctaaaccgct gagaactgtc gggtttatcg acgatcacac
     5761 cagaaatgtc cagcagcagt gcgccacgac tggccgtttc tttcgaatca ccaccactga
     5821 cctgcatcca gatccccaca ccaaccagta cgaggaaaat aaagaacagg ttaagtacca
     5881 tttcacggac gaaattcagc agacgccacg tccatttaaa aaatccggca ataaatcgcc
     5941 aaagggttcg catgtattct cccaacttaa ggtcacacct gtcacaggcg caatagatgt
     6001 ggttatcgta atgacacaac cggcacttgt cagcaggaat tgcggactgt gctgtaacaa
     6061 aatccactct cgtgttaatt ttgtgaataa atatcacgac aggagttaat caaatggatg
     6121 cactcgaact attgatcaat cgccgtagcg cctcccgcct ggctgaaccc gcgccaacgg
     6181 gtgaacaact gcaaaacatc ctgcgtgcgg gtatgcgtgc gccggaccat aagtccatgc
     6241 aaccgtggca tttttttgtg attgaagggg aagggcgcga gcgtttcagc gccgtactgg
     6301 aacagggggc gattgctgcc ggtagtgatg acaaagctat cgacaaagcc cgtaatgcgc
     6361 cgttccgcgc accgctcatc atcacggtgg tggcgaaatg cgaagaaaat cataaagtcc
     6421 cgcgatggga acaggaaatg tctgccggat gcgcggtcat ggcgatgcaa atggcagccg
     6481 ttgcccaggg atttggcggc atctggcgca gtggcgcatt aactgaaagt ccggtagtgc
     6541 gtgaagcatt cggttgccgt gagcaggata aaattgtcgg ttttctctac ctcggcacgc
     6601 cgcagttgaa agcatctacg tcgattaacg tcccggaccc gacgccgttt gtaacttatt
     6661 tctgataatt gtcgcgaaac tgtctggatt gtgagcaaag gcgcaggaat tcagacactc
     6721 tcacttatca cttcacggaa tgagggttac catacccgga ttgcattgac aggagatgtc
     6781 catgagcgag aactcgattc gtttgaccca atacagccac ggagctggtt gcggctgtaa
     6841 aatttcccca aaagtgttgg aaaccatcct gcacagtgag caggcgaagt ttgttgatcc
     6901 gaatttgctt gtgggtaatg agacccgcga cgatgcggcg gtgtacgatc tgggcaatgg
     6961 caccagcgtt atcagtacca ccgacttctt tatgccgatc gttgataatc ctttcgattt
     7021 tggccgcatt gcggcgacta acgccatcag cgatatcttc gcgatggggg gcaaaccgat
     7081 tatggcgatt gcgatcctcg gctggccgat taacaaactt tccccggaaa ttgcccgcga
     7141 agtaaccgaa ggtggacgct atgcatgccg tcaggcgggt attgcgctgg ctggcggtca
     7201 ctccatcgat gcgacggagc cgatttttgg tctggcggta acggggatcg taccgaccga
     7261 gcgggtgaag aaaaacagta ccgcacaagc cggatgcaaa ctgttcctga cgaaaccgct
     7321 ggggatcggc gttcttacca cggctgagaa aaaatcactg ctgaaaccag aacatcaggg
     7381 actagcgacg gaagtgatgt gccggatgaa catcgcaggc gcgtcctttg ccaacatcga
     7441 aggcgtaaaa gcgatgaccg acgttacggg ttttggtctg ctgggccatt tgagcgaaat
     7501 gtgtcagggc gcaggtgtgc aggcgcgcgt tgactatgac gcgatcccaa aattgccggg
     7561 ggttgaagag tacattaagt tgggcgcagt gcctggcggc accgaacgta actttgccag
     7621 ctacggtcat ctgatgggtg aaatgccgcg tgaagtgcgc gatttgctgt gcgatccgca
     7681 aacttctggc ggtttgctgc tggcggtcat gccggaagca gaaaatgagg tcaaagctac
     7741 agccgccgag tttggcattg aactgacggc aattggcgaa ctggtgccag cgcgcggcgg
     7801 tcgtgccatg gttgagattc gttaattcaa tgcggttgtt tattgccgaa aaaccgagtc
     7861 tggcgcgcgc cattgctgat gtcctgccca aaccgcaccg gaaaggcgat ggctttatcg
     7921 agtgcggtaa tggtcaggtg gtgacctggt gtatcggtca cctgcttgag caggcgcagc
     7981 cagacgccta cgacagccgc tatgcgcgct ggaatcttgc ggatttgccg attatcccgg
     8041 aaaagtggca attacagccc cgaccctccg tgaccaaaca acttaacgtc atcaaacgct
     8101 tcctgcatga agccagcgaa atcgttcacg ccggggaccc ggatcgtgaa gggcaactgc
     8161 tggtggatga agtgctggac tatctgcaac tggcaccgga aaagcgccag caggtgcagc
     8221 gttgcttgat aaacgacctg aacccgcagg cggttgagcg ggcgctcgac cgtcttcggt
     8281 ccaacagtga gtttgtgcca ttgtgcgttt ctgcgctggc gcgagcgcgt gccgactggc
     8341 tgtacggcat caatatgacc cgtgcgtata ccattctcgg tcgcaatgcc ggttatcagg
     8401 gcgtactttc cgtgggacgc gtgcagacac ccgtgctcgg gctggtggtg cgccgcgatg
     8461 aagagattga aaacttcgtg gcgaaagact tctttgaagt caaagcacat atcgtgacac
     8521 ctgccgatga gcggtttacc gctatctggc aaccgagcga agcgtgtgaa ccgtaccagg
     8581 atgaagaagg gcgcttgtta catcgtccac tggcggagca tgtggttaac cgcattagtg
     8641 gtcaaccagc aattgtcacc agctataacg ataaacggga atcagaatcc gcgccgctgc
     8701 ctttttcgct ttcggcgttg cagattgaag cggcaaaacg ctttggtctg agcgcgcaga
     8761 acgtgcttga tatctgccag aagctgtacg aaacgcacaa gctaatcact tatccgcgtt
     8821 ccgattgtcg ctatttgcca gaagaacatt ttgccggacg ccacgcggtg atgaatgcca
     8881 tcagcgttca tgcaccagat ctgttgctgc agccagtggt agatccagat atacgcaacc
     8941 gctgttggga tgacaaaaag gtcgatgcac accacgccat catcccgacc gcacggagtt
     9001 cagccatcaa cctgacggag aacgaagcga aggtctataa cctgattgcc cgtcagtact
     9061 tgatgcagtt ctgcccggat gtggtgttcc gcaagtgtgt tatcgaactg gacattgcca
     9121 aaggcaaatt tgtcgctaaa gcgcgttttc ttgctgaagc aggctggcgc acgctgttag
     9181 gcagcaaaga gcgcgatgaa gaaaacgacg gtacgccatt gcctgtggtg gcgaaaggcg
     9241 atgagttgct gtgtgaaaaa ggtgaagtgg tagagcggca aacccagccg ccgcgccatt
     9301 ttaccgatgc aacactgctt tcggcgatga ccgggatcgc gcgctttgtg caggacaaag
     9361 atctgaaaaa gatccttcgt gcgaccgatg gtctggggac agaagcaacc cgtgccggga
     9421 ttattgaact gctgttcaag cgtggttttc tgaccaaaaa agggcgctat atccactcta
     9481 ccgacgccgg aaaagcgtta ttccattcgc tgccagaaat ggcgacgcga ccggacatga
     9541 ccgcgcactg ggaatcggtg ctgacgcaaa tcagcgaaaa gcagtgtcgc tatcaggact
     9601 ttatgcagcc gctggtgggg acgctatatc agcttattga tcaagccaaa cgtacgccgg
     9661 tgcggcagtt tcgcggcatt gtggctccgg gcagtggtgg cagtgctgat aagaaaaagg
     9721 ctgcaccgcg taaacgtagt gcgaaaaaaa gtccgccagc agatgaagtc ggaagcgggg
     9781 cgatagcgta agcgagtgaa tctttcgtgc tattcgagt
//
LOCUS       DAJVQG010000142         9578 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJVQG010000142 DAJVQG010000000
VERSION     DAJVQG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9578
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.142"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(109..1086)
                     /gene="epmA"
                     /locus_tag="OQM83_003251"
     CDS             complement(109..1086)
                     /gene="epmA"
                     /locus_tag="OQM83_003251"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCS1521444.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1411..3219
                     /gene="frdA"
                     /locus_tag="OQM83_003252"
     CDS             1411..3219
                     /gene="frdA"
                     /locus_tag="OQM83_003252"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCS1521445.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3212..3946
                     /gene="frdB"
                     /locus_tag="OQM83_003253"
     CDS             3212..3946
                     /gene="frdB"
                     /locus_tag="OQM83_003253"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCS1521446.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            3957..4352
                     /gene="frdC"
                     /locus_tag="OQM83_003254"
     CDS             3957..4352
                     /gene="frdC"
                     /locus_tag="OQM83_003254"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCS1521447.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4363..4722
                     /gene="frdD"
                     /locus_tag="OQM83_003255"
     CDS             4363..4722
                     /gene="frdD"
                     /locus_tag="OQM83_003255"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCS1521448.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            4785..5918
                     /locus_tag="OQM83_003256"
     CDS             4785..5918
                     /locus_tag="OQM83_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCS1521449.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6007..6540
                     /gene="blc"
                     /locus_tag="OQM83_003257"
     CDS             6007..6540
                     /gene="blc"
                     /locus_tag="OQM83_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCS1521450.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6537..6854)
                     /gene="sugE"
                     /locus_tag="OQM83_003258"
     CDS             complement(6537..6854)
                     /gene="sugE"
                     /locus_tag="OQM83_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCS1521451.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7029..7175)
                     /gene="ecnB"
                     /locus_tag="OQM83_003259"
     CDS             complement(7029..7175)
                     /gene="ecnB"
                     /locus_tag="OQM83_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCS1521452.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7286..7411)
                     /gene="ecnA"
                     /locus_tag="OQM83_003260"
     CDS             complement(7286..7411)
                     /gene="ecnA"
                     /locus_tag="OQM83_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCS1521453.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7463..8029)
                     /gene="efp"
                     /locus_tag="OQM83_003261"
     CDS             complement(7463..8029)
                     /gene="efp"
                     /locus_tag="OQM83_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCS1521454.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8071..9099
                     /gene="epmB"
                     /locus_tag="OQM83_003262"
     CDS             8071..9099
                     /gene="epmB"
                     /locus_tag="OQM83_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCS1521455.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9489..>9578
                     /locus_tag="OQM83_003263"
     CDS             9489..>9578
                     /locus_tag="OQM83_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521456.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPR"
BASE COUNT         2416 a         2450 c         2448 g         2264 t
ORIGIN      
        1 caaatatcca gtcttaaatt gtggccttta acggaaaaat gaaaacatat gctacagaat
       61 ggcgcttatc acgccattct tcgctgttaa ttcagtaatt tttcagaatt atgcccggtc
      121 aacgctaaag gcgatgactt cagccagtgt ctccgcgccc agcgccaaca tcaccagacg
      181 atcaacacct aatgccacgc cggaacagtc aggcataccg actttcaagg cttcaatcag
      241 attctggtca atggggtgct gcggcaaacc gcgcgccgcg cgcttacggt tatcttgttc
      301 aaagcgttgt tgctgctcac gggcatccgt taattcatgg aaaccattcg ccagctcaat
      361 acctttataa taaacctcaa agcgttcagc gacccgatga tcttcggtac tgatttgcgc
      421 cagtgatgcc tggctggctg gaaagtggta cacaaaggtc ggtttttctt tgccaatatt
      481 tggctctacg ccaaaggtaa acagcaattg tagcaacgtg tcgcggtctt cttcggtatc
      541 agcaacgtta ctcaaatcca gtttcgctgc gacttcccgc agttgcgttt tgtcggcaga
      601 gagcgggtca atttccagat aacgcaagaa agcttgttga taagaaaggc tttctgctgc
      661 cgggcagtcc agcacctgtt gtaagagatc gtccacctcg ttcatcaacc ggtacatatc
      721 atagtgcggt cgataccact caagcatagt gaactcaggg ttgtgataac gccccatctc
      781 ttcattacgg aagctgcggc acagctggaa taccggccca caaccggcaa ccagcaggcg
      841 tttcatatgg tattccgggc tggtcattaa ccagagattc atcccctgcg aatgcccggg
      901 gccaacgaaa cgtgtctcaa acgggaccaa atgaatatcg gttaccgtcg cctggctcat
      961 acaaggcgtc tccacctcca gcactccacg atcggcaaag aaacgacgga tctccgccat
     1021 aatcgccgca cgttttaata agttaggaat ggatgcgctc ggctgccagg atgccgtttc
     1081 gctcatagtt aaatctccag tttttgacaa gggcacgaag tctactcgca acgcgacggc
     1141 gagacaaatt ttacgcagga atcaaacagc ggtgggcagt gactaaaaaa agcacgatct
     1201 gatggtttag taattaaatt aatcatcttc agtgataatt tagccctctt gcgcactaaa
     1261 aaaatcgatc tcgtcaaatt tcagacttat ccatcagact atactgttgt acctataaag
     1321 gagcagtgga atagcgttcg cagaccgtaa ctttcaggca cttaccctga agtacggggc
     1381 tgtgggataa aaacaatctg gaggaatgtc gtgcaaacct ttcaagccga tcttgccatt
     1441 gtaggcgccg gtggcgcggg attacgtgct gcaattgctg ccgcgcaggc aaatccaaat
     1501 gcaaaaatcg cactaatctc aaaagtatac ccgatgcgta gccataccgt tgctgcagaa
     1561 gggggctccg ccgctgtcgc gcaggatcat gacagcttcg aatatcactt tcacgataca
     1621 gtagcgggtg gcgactggtt gtgtgagcag gatgtcgtgg attatttcgt ccaccactgc
     1681 ccaaccgaaa tgacccaact ggaactgtgg gggtgcccat ggagccgtcg cccggatggt
     1741 agcgtcaacg tacgtcgctt cggcggcatg aaaatcgagc gcacctggtt cgccgccgat
     1801 aagaccggct tccatatgct gcacacgctg ttccagacct ctctgcaatt cccgcagatc
     1861 cagcgttttg acgaacattt cgtgctggat attctggttg atgatggtca tgttcgcggc
     1921 ctggtagcaa tgaacatgat ggaaggcacg ctggtgcaga tccgtgctaa cgcggtcgtt
     1981 atggctactg gcggtgcggg tcgcgtttat cgttacaaca ccaacggcgg catcgttacc
     2041 ggtgacggta tgggtatggc gctaagccac ggcgttccgc tgcgtgacat ggaattcgtt
     2101 cagtatcacc caaccggtct gccaggttcc ggtatcctga tgaccgaagg ctgccgtggt
     2161 gaaggcggta ttctggtcaa caaaaatggc taccgttatc tgcaagatta cggcatgggc
     2221 ccggaaactc cgctgggcga gccgaaaaac aaatatatgg aactgggtcc acgcgacaaa
     2281 gtttctcagg ccttctggca cgaatggcgt aaaggcaaca ccatctccac gccgcgtggc
     2341 gatgtggttt atctcgactt gcgtcacctc ggcgagaaaa aactgcatga acgtctgccg
     2401 ttcatctgcg aactggcgaa agcgtacgtt ggcgtcgatc cggttaaaga accgattccg
     2461 gtacgtccga ccgcacacta caccatgggc ggtatcgaaa ccgatcagaa ctgtgaaacc
     2521 cgcattaaag gtctgttcgc cgtgggtgaa tgttcctctg ttggtctgca cggtgcaaac
     2581 cgtctgggct ccaactccct ggcggaactg gtggtcttcg gccgtctggc cggtgaacaa
     2641 gcgacagagc gtgcagcaac tgccggtaat ggcaacgaag cggcaattga agcgcaggca
     2701 gctggcgttg aacaacgtct gaaagatctg gttaaccagg atggcggcga aaactgggcg
     2761 aagatccgcg acgaaatggg cctggcaatg gaagaaggtt gcggtatcta ccgtacgccg
     2821 gaactgatgc agaaaaccat cgacaagctg gcagagctgc aggaacgctt caagcgcgtg
     2881 cgcatcaccg acacctccag cgtgttcaac accgacctgc tctacaccat tgaactgggc
     2941 cacggtctga acgttgctga atgtatggcg cactccgcaa tggcacgtaa agagtcccgc
     3001 ggcgcacacc agcgtctgga cgaaggttgc accgagcgtg acgacgtcaa cttcctcaaa
     3061 cacaccctcg ccttccgcga tgctgatggc acgactcgcc tagagtacag cgacgtgaag
     3121 attactacgc tgccgccagc taaacgcgtt tacggtggcg aagcggatgc agccgataag
     3181 gcggaagcag ccaataagaa ggagaaggcg aatggctgag atgaaaaacc tgaaaattga
     3241 ggtggtgcgc tataacccgg aagtcgatac cgcaccgcat agcgcattct atgaagtgcc
     3301 ttatgacgca actacctcat tactggatgc gctggtctac atcaaagaca acctggcacc
     3361 ggacctgagt taccgctggt cctgccgtat ggcgatttgt ggttcctgcg gcatgatggt
     3421 taacaacgtg ccaaaactgg catgtaaaac cttcctgcgt gattacaccg acggtatgaa
     3481 ggttgaagcg ttagctaact tcccgattga acgcgatctg gtggtcgata tgacccactt
     3541 catcgaaagt ctggaagcga tcaaaccgta catcatcggc aactcccgca ccgcggatca
     3601 gggtactaac atccagaccc cggcgcagat ggcaaagtat caccagttct ccggttgcat
     3661 caactgtggt ctgtgctacg ccgcgtgccc gcagtttggc ctgaacccag agttcatcgg
     3721 tccggctgcc attacgctgg cgcatcgtta taacgaagat agccgcgacc acggtaagaa
     3781 ggagcgtatg gcgcagttga acagccagaa cggcgtatgg agctgtactt tcgtgggcta
     3841 ttgctccgaa gtctgcccga aacacgtcga tccggctgcg gccattcagc agggcaaagt
     3901 agaaagttcg aaagactttc ttatcgcgac cctgaaacca cgctaaggag tgcaacatga
     3961 cgactaaacg taaaccgtat gtacggccaa tgacgtccac ctggtggaaa aaattgccgt
     4021 tttatcgctt ttacatgctg cgcgaaggca cggcggttcc ggctgtgtgg ttcagcattg
     4081 aactgatttt cgggctgttt gccctgaaaa atggcccgga agcctgggcg ggattcgtcg
     4141 actttttaca aaacccggtt atcgtgatca ttaacctgat cactctggcg gcagctctgc
     4201 tgcacaccaa aacctggttt gaactggcac cgaaagcggc caatatcatt gtaaaagacg
     4261 aaaaaatggg accagagcca attatcaaaa gtctctgggc ggtaactgtg gttgccacca
     4321 tcgtaatcct gtttgttgcc ctgtactggt aaggagcctg agatgattaa tccaaatcca
     4381 aagcgttctg acgaaccggt attctggggc ctcttcgggg ccggtggtat gtggagcgca
     4441 attattgcgc cggtgatgat cctgctggtg ggtattctgc tgccactggg gctgtttccg
     4501 ggtgatgcgc tgagctacga gcgcgttctg gcgttcgcgc agagcttcat tggtcgcgta
     4561 ttcctgttcc tgatgatcgt tctgccgctg tggtgtggtt tacaccgtat gcaccacgcg
     4621 atgcacgatc tgaaaattca cgtgcctgcg ggcaaatggg ttttctacgg tctggctgct
     4681 atcctgacag ttgtcacgct gattggtatc gttacaatct aacgtatcgc caatgtaaat
     4741 ccggcccgcc tatggcgggc cgttttgtat ggaaaccaga ccttatgttc aaaacgacgc
     4801 tctgcgcctt attaattacc gcctcttgct ccacatttgc tgcccctcaa caaatcaacg
     4861 atattgtgca tcgcacaatt accccgctta tagagcaaca aaagatcccc ggtatggcgg
     4921 tggcggtaat ttatcagggt aaaccttatt actttacctg gggctatgcg gacatcgcca
     4981 aaaagcagcc cgtcacacag caaacgttgt ttgagttagg ttcggtcagc aaaacattta
     5041 cgggcgtgct tggtggcgac gctattgctc gaggggaaat caagttaagc gatcccgcaa
     5101 caaaatactg gcctgaactt actgctaaac agtggaatgg gatcacacta ttacatcttg
     5161 cgacctacac cgctggcggc ctgccattgc aggtgccgga tgaagtgaaa tcctcaagcg
     5221 acttgctgcg cttctatcaa aactggcagc ctgcatgggc tccaggaaca caacgtctgt
     5281 atgccaactc cagtatcggt ttgttcggcg cactggctgt gaagccgtct ggtttgagtt
     5341 ttgagcaggc gatgcaaact cgtgtcttcc agccactcaa actcaaccat acgtggatta
     5401 atgtacctcc cccagaagaa aagaattacg cctggggata tcgcgaaggt aaggcagtgc
     5461 atgtttcgcc aggggcgtta gatgctgaag cttatggtgt gaagtcgacc attgaagata
     5521 tggcccgctg ggtgcaaagc aatttaaccc cccttgatat caatgagaaa acgcttcaac
     5581 aagggataca actggcacaa tctcgctact ggcaaaccgg cgatatgtat cagggcctgg
     5641 gctgggaaat gctggactgg ccggtcaatc ctgacagcat cattaacggc agtgacaata
     5701 aaattgcact ggcagcacgc ccggtaaaag cgattaagcc cccaactcct gcagtacgcg
     5761 catcatgggt acataaaacg ggggcgaccg gcggatttgg tagctatgtc gcgtttattc
     5821 cagaaaaaga gctgggtatc gtgatgctgg ctaacaaaaa ctatcccaat ccagcgagag
     5881 tcgccgccgc ctgtcagatt ctcaacgctc tacagtaaaa tttcatcggg tccgaatttt
     5941 cggacctttt ctgcgctttt ccttgctgtc atctacactt agaaaaaaac cagtaaggaa
     6001 acatttatgc gcctgctccc tctcgttgcc gcagcgacag ctgcatttct ggtcgttgcc
     6061 tgcagttctc ctacgccgcc gcgtggcgtg acagtagtga ataatttcga cgctaaacgc
     6121 tatctcggta cctggtatga gattgcccgt tttgatcacc gttttgaacg tggactggaa
     6181 aaagtcaccg caacatacag tctgcgtgat gacggcggcc tgaatgtcat caataaaggc
     6241 tataaccctg acagagaaat gtggcagcag agtgaaggga aagcgtactt taccggcgca
     6301 ccaacacgcg ctgcgctgaa agtgtcattc tttggtcctt tctatggcgg ttataacgtc
     6361 attgcacttg atcgggaata ccgccatgcg ctggtttgcg gcccggaccg cgactacctg
     6421 tggatactct cccgcacgcc aaccatttct gacgaagtga aacaggagat gctggcagtc
     6481 gcgacccggg aagggtttga tgtcagtaag tttatttggg tacagcagcc cggtagttag
     6541 tgagtgctga gtttcagacc aataatcccc aatacgatta acgccagact cgccaggcgc
     6601 atcgggttag cggactcacc gagcagcaca atgccggtga tggccgcgcc gactgcgcca
     6661 atacccgtcc acacggcata agccgtccct accggtaacg atttcatcgc ccaggcaagt
     6721 agcgccatac tgactatcat cgccgtcaca gtaataacac ttggcgtcaa acgactaaag
     6781 ccgtgggtat atttcaggcc aacggcccat accacttcca gcagaccagc aataactaag
     6841 ataatccagg acatatcagg ctccggagaa ttggggccat ccccggtgaa aagaggcgtt
     6901 tgcaggtcgt cctgcaaagc tgatggttga acggatattt tgccaggtgg caggggaatt
     6961 tcaactatta cctttgtgac tatcgcaaaa aaacaaacgg cacgacataa ctgtcgtacc
     7021 gttattgctt attgctgcgc tttcgttgct gcaccagaaa tcgcgttacc gccatcagaa
     7081 atgtcttcac caacgccacg cgtggtgttg caggcagtta atactgttga aagcaccaga
     7141 acagaaaaga tcgctgcaat tgtcttcttc accataacgt cttcctttta ggtaatgtta
     7201 tttatgtttg cctatagcaa attaataata gacaacatcg ccaaaaatga tggaaatctg
     7261 atgatttttc ggaagagaag aaaaattagc tggcagcgcg ggagatggag ttgccgagat
     7321 gtttgatgtc ttcgccgaaa ccgcgagcgg tgttacagcc cgtgagcagc gtgctggcaa
     7381 gcaaaacaag aacgataagg cgtttcatca tctctgacct gaataagtga tggtgcagcc
     7441 tgcaggccgc accacaaccg cattacttca cgcgagagac gtattcacca gagcgggtat
     7501 ccactttgat gacttcgccg atttgtacga acagcggaac tttaaccaca gcgccagtag
     7561 acagggtagc cggtttgccg ccagtacctg cggtatcacc tttcaggccc ggatcggtat
     7621 caacgatttc cagttcaacg aagttcggcg gagtaacgga gatcggctga ccattccaca
     7681 gagttacgat acactctgcc tgatccagca gccatttagc gttgtcgcca attgcttttg
     7741 catcagcaga cagctgctcg aaagtttcgt tgttcatgaa gtgccagaac tcaccgtcgt
     7801 tgtacaggta agtcaggttc atatcgacaa catcagcgcc ttcagcggaa tcagtagatt
     7861 tgaaggtttt ttctacgcga gtaccggtca gcagacgacg cagtttaacg cgagcaaatg
     7921 cctggccttt acccggtttt acgaattcac tcgcttcaac cgcgtaaggt tcgccgtcta
     7981 acatgatttt aagaccagca cgaaaatcgt tgctatagta cgttgccata aggccctctg
     8041 aaatttgtta attggtagct aagccacaaa atggcgcata ttgtaaccct aaatacccca
     8101 tccagagaag attggttaac gcaacttgcc gatgttgtga ccgatcctgg tgaacttctg
     8161 cgtcttttga atatagacgc ggacgaaaaa ctgttggccg gacgcagcgc caaaaagctg
     8221 tttgccctgc gtgtgccccg ctcatttatc gatcgcatgg agaaaggcaa tccggacgat
     8281 cctattttgc gtcaggtact tacctcgcaa gatgagtttg tcgtcgcgtc cggattctcc
     8341 accgacccac tggaagaaca gcacagcgta gtgcctggtt tgttgcataa ataccacaac
     8401 cgggcgcttt tgctggtcaa aggcggctgc gcggtaaatt gccgctattg cttccgtcgc
     8461 cacttcccct atgccgaaaa tcagggcaac aagcgtaact ggcagactgc acttgagtat
     8521 gttgctgcgc atccggaact ggacgagatg attttctccg gcggcgatcc gctgatggcg
     8581 aaagatcacg agctggactg gttgctcaca caactggaag ccatcccgca tatcaaacgt
     8641 ctgcggattc acagccgtct gccgattgtg atcccggcac gtatcaccga ggcgctggtt
     8701 gaacgctttg cccgttctac gctgcaaatc ttgctggtga atcacatcaa tcatgccaat
     8761 gaggtagatg aaacgttccg tcaggcgatg gctaaattgc gccgtgtcgg tgtcaccctg
     8821 cttaaccaga gcgttctgtt acgtggtgtg aacgataacg cacaaacgtt ggcaaacctg
     8881 agtaatgcgt tgttcgatgc cggcgtgatg ccgtattacc tgcatgtgct agataaagta
     8941 cagggcgcgg cgcattttat ggtgagtgat gacgaagcac ggcagattat gcgtgagttg
     9001 ctgacattgg tgtcgggata tctggtgccg aaactggcgc gagaaattgg cggcgaaccc
     9061 agcaaaacgc cgctggatct ccagctacgc cagcagtaac tgattaaaca gggaagttaa
     9121 acattagcct gatgcgctac gcttatcagg cctacattgt gcattgaaat tgaatggatt
     9181 tgcaatgaac tgtaggccgg ataaggcgtt tacgccgcat ccggcaagag caacaaacgc
     9241 aagtacagca acctgtcatt aaactgggaa atttaatctt tgcccgttgc ataaccttta
     9301 attattcata tacattgctg ttgttactct ttatatagcc aacaccaata attaaccttt
     9361 cttttctgtt tatatagagt gagtacggct atttatgtta ttttaacaac tattactacc
     9421 aagcccattt atctggctta taaattaaaa ttaattattt aattgagaaa tggttaggga
     9481 gaacctacat ggccataagc atcaagggtg ttaataccgg tgttattcgc aagagtaata
     9541 atttcattgc actggcgctg aaaattaaag aacctcgc
//
LOCUS       DAJVQG010000143         9565 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJVQG010000143 DAJVQG010000000
VERSION     DAJVQG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9565
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.143"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            19..1344
                     /locus_tag="OQM83_003264"
                     /pseudo
     CDS             19..1344
                     /locus_tag="OQM83_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            1347..2675
                     /gene="ssnA"
                     /locus_tag="OQM83_003265"
     CDS             1347..2675
                     /gene="ssnA"
                     /locus_tag="OQM83_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HCS1521457.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            2726..3505
                     /locus_tag="OQM83_003266"
     CDS             2726..3505
                     /locus_tag="OQM83_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HCS1521458.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            3502..6441
                     /locus_tag="OQM83_003267"
     CDS             3502..6441
                     /locus_tag="OQM83_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HCS1521459.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDTCGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            6374..6688
                     /locus_tag="OQM83_003268"
     CDS             6374..6688
                     /locus_tag="OQM83_003268"
                     /inference="COORDINATES: protein motif:HMM:NF019581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCS1521460.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            6724..7074
                     /gene="ghxQ"
                     /locus_tag="OQM83_003269"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             6724..7074
                     /gene="ghxQ"
                     /locus_tag="OQM83_003269"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(7143..7631)
                     /locus_tag="OQM83_003270"
     CDS             complement(7143..7631)
                     /locus_tag="OQM83_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCS1521461.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVPTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(7631..9550)
                     /gene="ygfT"
                     /locus_tag="OQM83_003271"
     CDS             complement(7631..9550)
                     /gene="ygfT"
                     /locus_tag="OQM83_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HCS1521462.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
BASE COUNT         2307 a         2564 c         2493 g         2201 t
ORIGIN      
        1 gatccttaac tgatcggcat tattcctcag ttccgtattc ctgcagagtt aattcagcac
       61 gatatcgatt ttgttgccgc tcacggcgtg aaatttgagt atggctgctc acccgattta
      121 accgttgaac agttaaaaaa tcagggcttc cactatgttc tgattgccac cggcactgat
      181 aaaaatagcg gtgtgaaact ggcgggcgac aaccaaaatg tctggaaatc actccccttc
      241 ctgcgtgaat acaacaaggg tacagcgctc aagctgggca aacatgtggt cgttgtcggg
      301 gcgggtaaca ccgcaatgga ctgcgctcgt gcggcgttac gcgttccagg cgtagaaaaa
      361 gcaacgatcg tttaccgtcg ttcactacaa gagatgcccg catggcgcga agagtatgaa
      421 gaagcgttgc acgacggcgt agagttccgt ttcctgaata atccggaacg tttcgatgct
      481 gatggcacct taaccttgcg cgttatgtcg cttggcgaac cggatgagaa aggtcgtcgc
      541 cgtccggttg aaaccaacga aacagtaaca ctgcatgtag acagcctgat caccgccatt
      601 ggtgaacagc aggatactga agccctgaat gcgatgggcg tgccgctgga caaaaacggc
      661 tggccagacg tcgaccataa tggcgaaact cgtctgactg acgtctttat gatcggcgac
      721 gtacagcgcg gaccatcctc cattgtcgct gctgtcggaa ccgcgcgtcg ggcgaccgat
      781 gccatcctta gtcgggaaaa tatccgttcc caccagaacg ataaatactg gaacaacgtc
      841 aatccagcgg aaatctatca acgtaaaggc gatatctcta tcacgctggt gaacagtgac
      901 gatcgtgacg cgtttgtcgc ccaggaagcc gctcgctgcc tcgaatgtaa ctacgtttgc
      961 aacaagtgtg tggatgtctg cccgaaccgc gccaacgtct ccattgcagt ccctggcttc
     1021 cagaatagat tccagacgct gcacctcgac gcttactgta acgaatgcgg caactgcgct
     1081 cagttctgtc cgtggaacgg taaaccatac gaagacaaaa tcaccgtctt cagcctggcg
     1141 caagactttg ataacagcag caccccaggc ttccttgtgg aagattgccg ggtacgagta
     1201 cgtctgaata accaaagctg ggtgttaaac atcgacagcg aaggtcagtt taacaacgta
     1261 ccaccggagc tgaacgatat gtgccgcatc atcagccatg tccaccagca tcatcattat
     1321 ctgctgggcc gcgtggaggt gtaatcatgt tgattctgaa gaatgtcacc gcagtgcagt
     1381 tacacccggc gaaagtgcag gaaggcgttg atatcgctat cgaaaatgac gtgattgtcg
     1441 ctatcggcga tgccctgacg caacgctacc ccgatgccag ctacaaagag atgcatggtc
     1501 ggattgtgat gccgggaatt gtctgctcgc acaaccattt ttactcggag ctttcccgcg
     1561 gaattatggc aaacatcgcc ccttgcccgg atttcatctc aacgctgaaa aatctctggt
     1621 ggcggctcga tcgcgccctt gatgaagagt cgctctatta cagcggactg atttgttccc
     1681 tggaagcgat taagagcgga tgtacatcgg ttatcgatca ccatgcctcg ccggcgtata
     1741 tcggcgggtc gctctccaca ttgcgcgacg catttttaaa agttggcctg cgcgcgatga
     1801 cctgttttga aactactgac cgtaacaacg gcatcaaaga gttgcaggaa ggtgtagaag
     1861 aaaacatccg tttcgcccgt ttgattgatg aggcgaagaa agcgacaagc gagccgtatc
     1921 tggtggaagc acatatcggt gctcacgcgc cgtttaccgt gccggatgcc ggtctggaga
     1981 tgctgcgtga agccgtgaaa gccacaggcc gtggtttgca tattcacgct gcggaagacc
     2041 tttacgacgt ttcctacagt caccactggt acggcaaaga cctgctggca cgactggcgc
     2101 aattcgacct catcgacagc aaaacgctgg tcgctcatgg gctgtacttg tcgaaagatg
     2161 acatcgccct actcaatcag cgcgatgcgt tcctggtgca taacgcccgt tcaaacatga
     2221 acaaccatgt cggctacaac catcacctta gcgacatccg caacctggca ttgggaacgg
     2281 acggcattgg ttcggacatg tttgaagaga tgaaatttgc cttctttaaa catcgcgatg
     2341 cgggtggtcc gctgtggcct gacagttttg ccaaagccct gactaacggt aacgaactga
     2401 tgagccgcaa ctttggcgcg aaatttgggc ttctggaagc cggttacaaa gctgatttga
     2461 ccatttgcga ttacaactcg ccgacgccgc tgctggcaga caatatcgcc gggcatatcg
     2521 ctttcggtat gggctcaggc agcgttcaca gcgtgatggt caatggtgtg atggtctatg
     2581 aagaccgtca gtttaacttc gattgcgatt ccatttatgc acaagccaga aaagccgctg
     2641 ccagtatgtg gcgtcggatg gatgcgctgg cataaatgac agatgccctc ttcccgcagg
     2701 gaagagggtt aaataaggaa agaagatgat tgaacaattt ttcaggcccg actctgtcga
     2761 acaggcgctg gaactgaagc gccgctacca ggatgaagcc gtctggttcg ccgggggcag
     2821 caaactcaat gctacaccaa cccgtaccga taaaaagatt gccatttcct tacaggatct
     2881 ggaactggac tgggttgact gggataacgg tgcactgcgg attggtgcaa tgtctcgctt
     2941 gcagccactg cgtgatgcgc gctttattcc tgcagcactg cgtgaagccc tcggttttgt
     3001 ttactcacgc catgttcgta atcagtcgac cattggtggt gaaatcgccg cccggcagga
     3061 agagtcagtg ctgcttcccg tcctgctagc actggatgct gaactggttt ttggcaacgg
     3121 cgaaacgctg tcaatcgagg actacctggc ctgcccatgc gatcgcctgc taaccgaaat
     3181 tatcattaaa gatccgtatc gcacctgtgc gacccgcaaa attagccgtt ctcaggcagg
     3241 tttaaccgtc gtgacggcag ccgttgcaat gacagaccac gacggtatgc gaattgcgct
     3301 ggatggcgtc gccagtaaag cactgcgtct gcacgatgtc gaaaaacaaa atctggaagg
     3361 caatgcactt gaacaggctg tcgccaacgc cattttcccg caggaagatt tgcggggcag
     3421 cgtggcctat aaacgctata tcacgggagt tctggtagcc gacctgtatg ccgactgcca
     3481 acaggctggg gaggaagccg tatgatcatc cactttactt taaatggcgc gcctcaggag
     3541 ctaaccgtta atccaggcga aaacgtgcaa aagctgttgt ttaacatggg aatgcactct
     3601 gtacgcaaca gtgatgatgg cttcgggttt gccggttctg acgcaataat ctttaacggt
     3661 aatatcgtta acgcgtcctt gcttattgcc gcacagttag agaaggcaga tattcgtacc
     3721 gcagaatctc tgggtaaatg gaacgagtta agtctggttc aacaggcaat ggttgatgtt
     3781 ggcgtggtgc agtctggtta taacgatcca gctgcagctc tgattatcac cgatcttctc
     3841 gatcgcatcg ccgcacctac ccgcgaagag atcgacgacg cgctttctgg tttgttcagc
     3901 cgcgatgcag gctggcaaca atactatcag gtcattgaac tggcggttgc acgtaaaaat
     3961 aatccgcagg ccaccattga tatcgctccg actttccgtg acgaccttga cgtcattggc
     4021 aagcattatc ctaaaactga tgccgcgaaa atggtgcagg cgaaaccctg ctatgttgaa
     4081 gactgcgtaa cggctgacgc ctgcgtcatt aaaatgttac gtagcccaca cgctcacgca
     4141 ctgattactc atctggatgt cagcaaagct gaagccttac cgggcgtcgt tcacgttatt
     4201 actcacctga attgccctga tatctactat accccggggg gtcagagcgc accggaaccg
     4261 tcaccgcttg accgccgtat gttcggcaag aaaatgcgtc acgtcggcga tcgtgttgct
     4321 gcggtcgtcg cagaaagtga agaaattgcg ctcgaagcac tgaagctcat cgacgttgaa
     4381 tatgaagtgc ttaagccggt aatgtcgatc gacgaagcaa tggcggaaga tgcgcctgtc
     4441 gtgcacgatg aaccggtggt gtatgttgct ggtgcgccag atactctgga agacgataac
     4501 agccatgcag cccagcgcgg cgagcatatg atcatcaact tcccgatcgg ttctcgccct
     4561 cgcaaaaata tcgccgccag tattcatggt catattggcg atatggacaa aggctttgcc
     4621 gatgccgatg tgatcattga gcgaacctat aactcaacgc aggcacagca gtgcccgact
     4681 gaaacacata tctgctttac ccggatggac ggcgatcgtc tggttatcca cgcctccacc
     4741 caggtaccat ggcacttacg ccgccaggtc gcgcgcctcg tgggcatgaa acagcataaa
     4801 gttcatgtca ttaaagagcg agttggcggc ggttttggtt ccaaacagga catcctgctg
     4861 gaagaagtgt gcgcctgggc aacctgcgtg accgggcgtc cggtactatt ccgctacacc
     4921 cgtgaagaag agtttattgc taacacctct cgtcacgtcg cgaaagtcac cgtcaaactg
     4981 ggagcgaaaa aagatggtcg cctgacggca gtgaagatgg atttccgcgc caacactggc
     5041 ccttacggca accactcact caccgtaccg tgtaacggac cggcgctgtc gctgccgtta
     5101 tatccgtgcg ataacgtcga tttccaggtc accacctact acagcaacat ttgcccaaat
     5161 ggtgcttatc agggttatgg cgcaccgaaa ggtaacttcg ctatcaccat ggcattagcg
     5221 gaactggctg aacagttaca gatcgaccaa ctggaaatta tcgaacgtaa ccgggtacac
     5281 gaagggcaag agctgaaaat tctcggtgca atcggtgaag gtaaagcgcc gacctccgtt
     5341 ccttccgccg ccagctgtgc actggaagag atcctgcgtc agggtcgcga gatgatccaa
     5401 tggtcgtcac cgaaaccgca aaatggtgac tggcacatcg gtcgcggcgt cgccattatc
     5461 atgcagaaat cgggtatccc ggatatcgat caggctaact gcatgatcaa actggaatcg
     5521 gacggcacct ttatcgttca ttctggcggt gcggatattg gtactggtct ggatactgta
     5581 gtgacgaaac tggcagcaga agtgctgcac tgcccaccgc aggacgtgca tgttatctcc
     5641 ggtgataccg atcatgcgtt gtttgataaa ggcgcatatg cctcgtccgg tacttgcttc
     5701 tcgggtaacg cggcgcgttt ggcagcggaa aatctacggg agaaaattct gttccacggc
     5761 gcgcaaatgt tgggtgaggc agtggcagat gttcaactgg caacgccggg cgtcgtgcgc
     5821 ggcaagaaag gcgaagttag tttcggggat attgcccata aaggcgaaac cggcatcggc
     5881 tttggttcac tggtgggaac tggcagttat atcacgcctg atttcgcctt cccgtatggc
     5941 gcaaacttcg ctgaagttgc cgtcaacacg cgtacgggtg aaatccgcct ggataaattc
     6001 tacgccttgc tggactgcgg tacaccggtc aacccggaat tagcattggg acaaatctac
     6061 ggtgctaccc tgcgagctat cggccacagt atgagcgaag agatcattta tgacgccgaa
     6121 ggtcacccgt taacgcgtga tttacgcagt tacggcgcac cgaaaattgg tgacattccg
     6181 cgtgatttcc gcgctgtgct ggtgccgagc gacgataaag tcggcccgtt cggggcgaaa
     6241 tcgatctcgg aaatcggtgt aaatggcgca gctccggcga ttgctaccgc aattcacgat
     6301 acatgcggca tctgggcaga agaaagtcaa agtgggcatg ggaacggata tcggtgccgg
     6361 aacaactttc aacatgctgc aaacgctgaa cgaagcctac aaagtgttgc aattacaagg
     6421 ctatcgcctc tcggcatatg aagcgtttta cctggccacg ctcggcggag cgaaatctct
     6481 gggccttgac gatttgattg gcaacttttt acctggcaaa gaggctgatt tcgtggtgat
     6541 ggaacccacc gccactccgc tacagcagct gcgctatgac aactctgttt ctttagtcga
     6601 caaattgttc gtgatgatga cgttgggcga tgaccgttcg atctaccgca cctacgttga
     6661 tggtcgtctg gtgtacgaac gcaactaata ataaaacttt aacatcctcg tgaggacatc
     6721 attatgtctg gagacatcct acaaacaccg gacgcaccaa agccacaggg cgcgctggat
     6781 aattatttta aaattaccgc tcgtggcagt accgttcgtc aggaagtact ggctggctta
     6841 acgacctttc tggccatggt ttattccgtt atcgtcgttc cgggaatgct gggcaaagca
     6901 gtttttcctc ccgcagctgt gtttgttgcc acctgtctgg tcgcgggctt cggctcgttg
     6961 ctgatggggt tgtaggccaa tttgccaatg gcgattggtt gtgcgatttc cttgacggca
     7021 tttaccgcat tcagtctggt actcgggcaa caaattagcg ttcctgtcgc actggtcgca
     7081 ttttacgcgg gtggttgggc aatctaatag attctccgcg cctttcagcg cggagatttc
     7141 tttcacagag gattctcacc gctggcaaca atacgctgct ggcggactcg cctgagttct
     7201 ctttcagtaa gcaactgcag cgcctgtgtc gggcaagatt caacacaggc tggcccctct
     7261 tcccgctgtt cacagagatc gcatttcaca atttgttgtc gggtatcccc cggcaacgac
     7321 tgaatggtga tcattccaaa cgggcacgca ctgacgcaac tctgacagcc aatacaacgg
     7381 gcagaattgg tcggcaccac ctgctcgccc atcgtcagcg cccccacggg gcaagcgcca
     7441 acacaagggg cgttttcaca ctgatggcac atcaccggcg cactgatgct gtccagtcgc
     7501 tgtaccttca gccggggtaa aaagacatcg gcattcaact cctgttctga agggtgagcg
     7561 accacacagg ctacttcaca agtacggcag ccaatacagt cagccggatt aacgataatt
     7621 aacgatttca tcacgatgcc ttcgtatcaa acagagttaa catatcgcgc gccgcctgcc
     7681 ttcctgcggc cattgcagtg acaaccagat ccgcgccatg aactgcatca ccaccagcaa
     7741 agactttttt cagatgcgtc tgggtaggta aatacccgac gtcaccggtt tgaatcaggc
     7801 cccatttatc gagtttaatt ccgctgccct gcaaccacgg catggcatgc gcctggaaac
     7861 caaaggccat aatcagaaca tcggcgggca attcaaactc tgaacccgct accggacgag
     7921 gacgacggcg accatccggc cccggctcac ccatggcggt acgaatcagg cccaccgcag
     7981 ttaaacgccc atcttcgtca caagcgatat attgcggttg aacattgaac tgaaactcga
     8041 caccttcctc gcgcgcatta accacctctt tgcgcgagcc cggcatactg acttcatcac
     8101 gacgatacgc gcaggtcacg ctggcagcat tgaggcgaat ggaagtccgc aaacaatcca
     8161 ttgtcgtatc gccaccgccc aataccacga ctcgcttacc ttccacgtcc gtcagcggat
     8221 actcttcaga ctccggcaat cccatgagct ggcgggtatg ggcagtcagg aacggtagag
     8281 cctgaatgac accgggcgca tcttcatgcg gcagatctgc tcgcatcatc ccgtaagtcc
     8341 ccacgccgat gaaaactgca tcatattcag aagttaaatc gctaaaggta atatcgcggc
     8401 caatttcaca gttaagatga aagtcgattc ccattgcggt gaatatctct cgccgctggc
     8461 ttaataccgt tttatcgagt ttgaaaggag gaatgccaaa agtcagcata ccgccaattt
     8521 ctggatggcg atcaaagaca tcaacctgaa ctcccgcgcg cgccagaata tcagcacacc
     8581 ctaaccctgc tggcccagcg ccaatcaccg ccactttttc gatacgggga acaactttgc
     8641 tgacatcggg acgccagccc atcgccagcg cggtatcggt gatgtagcgt tccagattac
     8701 cgatagagac tgcgccagag tgatctttca aagtacatgc gccttcacaa agacggtcct
     8761 gtgggcatac cctgccgcag atttctggta aggaactggt ctggtggcaa agttctgccg
     8821 cttcaataat ctttccttcc tgtaccagac ggatgtaatc cggaatagcg ttatgcagcg
     8881 ggcaatgcca gttgcagtta gctttttcgg cacaataaac acagcggtca ctctcataag
     8941 tcgcttgcag tggatccagc ccgcaataga tttcgccaaa gtgggttttc cgttcactcg
     9001 ctgaaatttt atctgcgcct ttacgcgagt taacggggag caacgctgca ctgcgagatg
     9061 gctgagcgtc tgatgacgct tttcctgctg ccgttttgcg ctggcgagcc acctttatcc
     9121 actgtaaccc tttatcgtcc atcagtcgta acgcctgtgt tgggcagact tcaatacagg
     9181 cttgcgtgcc ggaactgcgc tggttacaaa ggtcgcattt ctgcgcaatc gtatcgacca
     9241 tctcaacgac gccaaagggg caagcgattg cgcatctttt acagccaata catttttgct
     9301 cgtccagttg tacgctatcg gactggaaag tcagagcatt aaccggacaa gccgtaacac
     9361 aaggggcatt gttgcagtga tggcaggcca ccggattcgc agcctggcct ttccctacaa
     9421 cgtggatacg cggtcgaaag tcactgtgac tcagcggcca gttttcttga ttgtgtgcca
     9481 ccgcacaggc aatttcacaa gcatgacagc ctatacattc cgcagcttca gcagcgataa
     9541 acttattcat ttgcatccct ttcat
//
LOCUS       DAJVQG010000144         9272 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJVQG010000144 DAJVQG010000000
VERSION     DAJVQG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9272
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.144"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(62..1144)
                     /locus_tag="OQM83_003272"
     CDS             complement(62..1144)
                     /locus_tag="OQM83_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HCS1521463.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1141..2937)
                     /locus_tag="OQM83_003273"
     CDS             complement(1141..2937)
                     /locus_tag="OQM83_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCS1521464.1"
                     /translation="MNVQHFDFFTDNEFSNLERYQTCKAMAKGYAGNYKTGQSRLMHA
                     RSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAERTGELIAQFPAE
                     DAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDFSHASVIDPACGG
                     GAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLVLLESVLMPLCVK
                     VKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKYSRSLFGHANLYG
                     LFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYALDFVTDRDGVFD
                     DVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKGGATWLLPRVKDD
                     ACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPLVWAESITSAGFR
                     FSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAILPQGFIDETGGV
                     VVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVSAYELNSIPLPSL
                     EQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3188..3544)
                     /locus_tag="OQM83_003274"
     CDS             complement(3188..3544)
                     /locus_tag="OQM83_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521465.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3602..4024)
                     /locus_tag="OQM83_003275"
     CDS             complement(3602..4024)
                     /locus_tag="OQM83_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCS1521466.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4040..4765)
                     /locus_tag="OQM83_003276"
     CDS             complement(4040..4765)
                     /locus_tag="OQM83_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HCS1521467.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4787..5533)
                     /locus_tag="OQM83_003277"
     CDS             complement(4787..5533)
                     /locus_tag="OQM83_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS1521468.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5540..6604)
                     /locus_tag="OQM83_003278"
     CDS             complement(5540..6604)
                     /locus_tag="OQM83_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HCS1521469.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6676..7101)
                     /locus_tag="OQM83_003279"
     CDS             complement(6676..7101)
                     /locus_tag="OQM83_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS1521470.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7098..7361)
                     /locus_tag="OQM83_003280"
     CDS             complement(7098..7361)
                     /locus_tag="OQM83_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1521471.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            7473..7973
                     /locus_tag="OQM83_003281"
     CDS             7473..7973
                     /locus_tag="OQM83_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1521472.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            7993..8187
                     /locus_tag="OQM83_003282"
     CDS             7993..8187
                     /locus_tag="OQM83_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCS1521473.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            8187..8477
                     /locus_tag="OQM83_003283"
     CDS             8187..8477
                     /locus_tag="OQM83_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCS1521474.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            8757..8909
                     /locus_tag="OQM83_003284"
     CDS             8757..8909
                     /locus_tag="OQM83_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCS1521475.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            8909..>9272
                     /locus_tag="OQM83_003285"
     CDS             8909..>9272
                     /locus_tag="OQM83_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521476.1"
                     /translation="MRLTMEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARK
                     IKTAFVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRR
                     GLDGSYDHDVLQVLRTMVT"
BASE COUNT         2383 a         2442 c         1968 g         2479 t
ORIGIN      
        1 tttttatcca gtgcaccgta aaacccccat ccttcagggc gtggcggata tcaaactttg
       61 gtcattttct ctggctgatc tttaccggat aaccatcccg aagatcaatt aagtgctccg
      121 gttcagaagc aaaccatgca taagatcccc acgccaattc tggtattgcc tttttaaaag
      181 gcagagcact tctgtccata aatgccgtaa ggaatgcaag atttttctcg ctgaacccag
      241 cgtcaatagc caaatgagtg agagtaactt ttcgttccct gtttataggc ccatctgtcg
      301 caactacttc agtgaaaact acaagcatgt ccgaacccgt tcggtcctct cccaaatcaa
      361 tcaaaataat atctggaagt gcttttgagg catcaatatt cagccccaat gcttttgcca
      421 atacatcatc ctgagcaaca actttattcc cggattcaga caaccagagc acagttggat
      481 tcttaagaaa attaggagca aaaacttcga tgacggcctt agctattgta cttgacgggc
      541 ccggagctaa agttctcttc tctccatttg ggaaagtgac gactacggca tcagcagcaa
      601 tcactgcccc tgatttcaat aaacgcaacc tagcaagagc ggctttgttc aaatgatttt
      661 cttgccagga acttattgca ctcagcagcg aatctccttc aagcccggca tcaaagagtg
      721 ctgcaaaaga gcgattcaaa ctgtaacgag gacgagagga tgtggtgggt atacctttac
      781 gctcaacaat tgcattgcaa ggtattaggc cattttttat agtttcatca cgtattggct
      841 cacgcgtatt ttccgcatac catgcatcag caggccttat cttttgttta gagaaagtca
      901 tctttaccca gctttctctg gacgcatcat cagtaagagc agcctgcgaa tctcccatac
      961 ttacaacctg gctgggcctt atccatcgcc ccagcccctc aaccgcacca gcataaaaca
     1021 tgacaaaaat tgtttttgca gctatttccc gaattaaata tccacgattc tcagtccctt
     1081 cagggaaaat taattgtaat ctttcccgga taacattcaa tgggggaaca acaggcagat
     1141 tcatgcaatc atcactccat agaaaccagc gatagttctc tcaataatcc gtctgtggac
     1201 gccagcatca attaacgatt gaatctccat cacctgctcc agagaaggca acgggattga
     1261 attcaattca taggccgaaa cagcaacact tccgcttata cacctgaacg cccgatcgac
     1321 aacatgcgaa ttaagtagca tatcaataac atcagggcga accgcagaga acaatccgtt
     1381 tgaatataca atattaatat ggttttcaac tactacgcca ccagtttcat ctataaatcc
     1441 ctgaggtaaa attgccgcta aaatccttct gtcctgttcc ttcgatgttg tccgctgaac
     1501 aagcacacat tcagattttg tcacaaggaa accctgttgt ggggttatat cgatataggg
     1561 aacatgattt tttctgtctg cactaaaacg aaaaccagct gaagttattg attccgccca
     1621 gactaatggg tagctatttt ttcctttcgt cgtacgcaac tgcgacttaa atcgattcca
     1681 gactaactga cctgtagaga ctgagtatcc cagatcagct aaacgcgtag acatctgttt
     1741 taatacatta agaaaacagg catcatcttt aacgcgtggc aaaagccacg ttgctccacc
     1801 tttttctata tcaacattac ctattttctc aatttttgct ttgttcaacc ccttagggac
     1861 aagtgaggat acctgggccc gaactttatg ttctcccgcc ttgaatgctg tcaacattgt
     1921 ttcctggaga acatcgtcaa agaccccatc cctgtcagtg acgaaatcaa gggcatacgg
     1981 agtagttttt tcggtcaaaa gggttcgcag cgcggtgaaa tactgcccac caagaaaaga
     2041 agttggcgta agaaatgcaa taactcctgt tttctcttta accatgcgaa cagctaagtc
     2101 ggtgaacaat ccatatagat ttgcatgacc aaataacgaa cgagaatatt tttctctcgt
     2161 tttaatatcg agagtgacac gtccgtaagg cggattccct accacaagat cataaccaga
     2221 aaatttatct tgctgaagcg catcagcaac aataattgta tcttcaggca atctacgttt
     2281 tacctttaca catagtggca ttagtacaga ttcgagcaat accaaactca tccaagccgc
     2341 aaatggatcg atctctatcc cctttaaacg acgactgatt ctcctcaaca tccattcagg
     2401 ggaagacccc ttgtcttttt ttaacattcg aatggctact ggtgccaaaa atgcaccgcc
     2461 accacacgca ggatcaataa ccgatgcatg agaaaaatca acaccagact tttcagccag
     2521 gtccaagaga cgcgctacca aaggtggggg ggtatagtaa gcccccaatt ccgaacggta
     2581 agcggatggc agcattaccg tataaattga cccaatcaga tatccagcgt cctccgccgg
     2641 aaattgagca ataagctcac ctgttcgttc agcgagctcg cgtgcatcac atggaatgtc
     2701 ttctatgtaa acagtgtgcg gtactgccct cattttaatg ctgctttttt gcttttttgc
     2761 aagggtatcc caatatgcag ctacgatgct gaagcagtat gagcgggcat gcatcaacct
     2821 tgactgcccg gttttgtaat tgcccgcata acctttggcc atggctttac aagtttgata
     2881 tctttccaga ttagaaaact cattatctgt aaaaaaatca aaatgttgca cattcataat
     2941 gtaaaatcct aatgtatcaa acatgcgggc cataacatct catggtggcg catagcaaac
     3001 tggcccgaag aatatcacgc accaagtcga tctcataacc cgttatgaca ataaatcttt
     3061 gtagctgaac tcacttggtg atttgtcaat cattcctggc ctccagttcg tcctgaatct
     3121 ctttatcgag ttcaccgtat ttgttagtcc tcatcactca cgacttgact tccagaaata
     3181 accttgatta ttcggggcta tattttgtca ccctgcgtat ccgcgctttc gcattacgct
     3241 caatctggat tagcttttct atattttttc gcctttcccg ctcctcctgg cgcaagagcc
     3301 ttacatcatc tgccagtctg gtttctcttt tcgccacaga gagcatccag tcaaatggct
     3361 ccacaactgc accgcaaatt ttacagcgga cctgacgctc tttttcgtca acccggacag
     3421 aagcgtgatg gcaatatggt ctttccgatg gctcataaag aaaattaacc tgattacgag
     3481 ggttatcctc ttttaccgga aataaaacaa tattgcttaa ctcatcttct ggttttattt
     3541 ccatgctcct ctcctttgat gcgaatgcca gcggtaattg aagcctgata gctaatttca
     3601 ctcacagtac cgcctcctga aaatcaccct gatagaaagc cggtacacgc tgcataactt
     3661 cactcttccg gcactcgcga cagattatgt tctgatgtct gtcgtagcgg cgtatttctc
     3721 cgtccggtaa tgaccagata aggtccggat caaccacaga tggtttcttc agctttgccc
     3781 tcgagagttt tttgcgggcg ttttgccagt ccttacgagc ctgttcagac gggaataacc
     3841 cgtagccaga gttgtataca tccccactgg caaccagctc tctggcgaga acgcttatta
     3901 aatatcttgt cgccccggtt ttagcttcca gttgtcgtaa cgtctcgcgc ccactctggc
     3961 gtacgagttc aacaacctgc cccttaattt tttcccgctc ttcctgtgta aatacttttg
     4021 ccataagcgc ccccggcaat cacttttccg acacaatacg accggaggaa tcgacaatct
     4081 gtcgaacaat atcccggtgc ttgttcagct cccgcaacgc ggcgcagaca cgctcccact
     4141 tctggacatg acttttcgcc cgacgcagtt cgcggtttgc catatgcagc gatggtaaaa
     4201 ccaggtcatc cgcttgcgtt tcggtgaacg atggcaacga ctgcacaatg tccgccacag
     4261 tttctgtttt aatttcttcc tgtgttgcag cttcctgtac cggtaacgca acacctgctg
     4321 gctgaggaaa gactttacca tccgtttccg ctatggatgc ggctttcggc tctgccggta
     4381 aatcagcgcc cggtatgcag taccgaaatt taccgccctg attcacgcga atcagacgcc
     4441 ctttgctgat tgccatggcc agcgatgaat tcgcccggcg ggaggtaatc ccgaacatca
     4501 gtgccagctc atccgccgtt tgtgggccat gttgttcaat cgcctcagtc agcatttgcg
     4561 ctgtcacttt cggtaccggt gacactggtt cactttcacc agcctgaatc agccaccaca
     4621 tcgaaccctt gttatccgct tcaccacggc gcttcagttt ccacagttcg ttgaccgcat
     4681 cttcacggct gattccaagg cgggacgcca ccacatgtga agaggctctt ttcagtgctt
     4741 tcagtgcgtc aaatacagtt tccattaaaa catcctccaa caaaaattac ttcacaactc
     4801 ccggaaagct gacatttgaa cgccagctat cccagttaaa cgtcacccac cgacaaccgt
     4861 tcatggtcat gcggtccata atcctctcac caagaagcgt gctcattgcg gcatgattca
     4921 ggtttgttaa catcccgaca ctgcacagtg atgctgtccg gcgatcaatt atctggtgca
     4981 ataccacctg ctcgtttttc gtctcccgct gaatgcctat ttcatccagg accagcaaat
     5041 caaccccgca aagctcctgt aaaaattttt caccggattt gccgttgtcg tagctgtcat
     5101 gcaacacgct catgacatca gactcggtga cgataatcac gctgcgcccc ttcgccatca
     5161 gccggttacc catcgccgct gcaaggtgat ttttcccggt gccggtttta ccgctgaaca
     5221 caaaattcgt gcacccggtc atcagttcgt cagctatgga tttggcctgg ctcagcgcgt
     5281 atttttgccc gtcgttctgc acctgataat tcgcaaacga gcatttgctg tgcagaggct
     5341 ggatgcccga acgattcagg attttttcca cccgcaactg gcgattctgg cggttaatct
     5401 cctcgctgcg ttttcgtcct tcagcaagtt gccattcccg ccactcctcc accgtccggt
     5461 acggtggaac cgccccctgt ggtgcaagtc tgtgaatacg ttcaagaacc ccggctgccg
     5521 caatgttttt catgccacat caccccctga atcccggcgg aatttcggta tccggttcag
     5581 aaatatgatt cacacaacgc tggttgttcg tgccgcttac cgggagcaac caggggtttt
     5641 caaaattccg gtccggccca aaaaacgtcg tcgctcgctg aacaaattcc gttcccgctt
     5701 tcccggtcgc cgccaggtat ctcgcgtaac gcctcacacc atccagcatg gtctctggtg
     5761 gcaccccctc gcgcaatctg gccttccagg cactgaatgc ggatttcttc gggtttgccc
     5821 cggcacgcaa cgggtattcc cgccagacct gttcgaacac atccggataa tccactcgtc
     5881 ccacaggctg cccggtgttt tccggggcta cccgatcggc ttcccgctga atggcggaat
     5941 cggcttcagg ctgctgcagt tggagtgatt gctccggcct tgcggtcatc acctgctgca
     6001 cagcgcccga atcggctttc agcgcatacg ctgaatcggc ttccggtgtc gagcctgctg
     6061 gctgaccagg attgacggtc tgaacatccc ctgcctggtt cgtggcgttt tttacgccat
     6121 ggaccatagt gttttgatct tctttatctg tatctttatc tgtatctgtc gtgactcgtc
     6181 gtgacatgtg cgtgacattt cgtgacgcgc cgtgacaatc gccattttgt tcccgctttc
     6241 tttccctctc tcgctgcgcc ctcttgcgct ctgcaggaga ttttgcggtt tgcgaaatat
     6301 tgccgttgtc ctctttaagc acctggcgtt tttcccatcc agtgattaaa tcaccatcaa
     6361 gtacccgccc ctgcatcgtc tgcaaaattg aatcaattac ctcttctgtc acgtcgagcg
     6421 cacttgccaa atcttctgtc gtgacatcaa tgtgacctcg cgtgacattt cgtgacgcgc
     6481 tcaccaggag gtggatatac actgccatca ctgttgcaat tggctgccct gacaccctgg
     6541 caattgttcg ccacttaggg tcatttggca tgtcatgcca taatctgagc caggcgttag
     6601 ccatactcac ctcttctgat accgaatctt tttactcacg agttgccggg agcgattcga
     6661 tatggctatt gtcagtcaat gtactgccac agcatttcct gccgggccac cacggttcat
     6721 ctgattgaaa ccggcgattg ccactgcgac aaaatcatca gcgtctctca ccagtcgttc
     6781 atgcgtctcc accagttccc gaaaataagc tgaactgtgg ctgcgcattc gagccaccag
     6841 cggaggtggc attgcccttt cgatcgcagg taacaacgcc tgaatttttt caacagcgtc
     6901 aggggtgtct ttatccagcc aacggaaaat cttttgggtg ttgagataaa gcgtgttgtg
     6961 cttgctgtcg ttatgcagaa ccggaaacgt cattcccagt tcgaaataaa ccctggttat
     7021 ttcagctgcc ggaacttttt caccgtccgg atgcgcccag gctttcatcg ccatgcggat
     7081 gtgttcatgc ttgattttca tgaatcaacc cccgccgatg gttgtgtgtt agcctgaaac
     7141 tcagcaggca agccgtcagt tgggttgggg taggttttac tgtcaatctc atgtggggtc
     7201 accacccatc ccgttgcttc acaccaacgt aatatttttt ctcctgtaag ttttgcacgc
     7261 cccgttataa cgtggctgac catcccttgg gatgcgccaa caatctcagc aaatgaactc
     7321 tgggttatat gaaaatgatg caaatattct ccaagattca taatccgccc tatgtgatgt
     7381 gttaccaatg cataattaat agcactgtta tttttaaaag taaatagtga tactatttca
     7441 aagtgattag caatcttatt agaattggat gtatgaaaag aaaatcattg tcagacaccg
     7501 acctcaacgc cgccagaaga ctgaaagaaa tctggatggc aaagaaatct cagttgggat
     7561 tgactcagga acgtgcggcg gaaattatgg ggttttcaac acaaggggcg gtaagtcact
     7621 acctaaatgg tcaaacccca ctaaaccttg aggctgttct aaagtttgct ggcttactga
     7681 aagttccccc tgagtcaatc aggccagaca tggtagattt gctgcaaatt gcaaggatgt
     7741 attgtcgaga accacagcaa gataacatag tcgcattacc agcagacaca gaacaaacag
     7801 aggatgaact accgtttgcc gtagagccga tggaacgaga tttaatccat acgttcagag
     7861 cttttccgaa agaagatcaa gaacacatgc ttcaggagat gaaagataaa aaagaatcaa
     7921 tggatcgtac ggttgcgcgg tggttagctg cacaaaaagg ccgtcgcgcc taaatcagag
     7981 gagatatgta aaatgagtac agtcctttcc ccaatggttt cagaatttga gacagttgag
     8041 caagaaaata gttacaacga atggctgcgg gccaaagtag cagcaagcct cgcggatccc
     8101 cgcccggcga ttccgcatga tgaggtaatg gcggaaatgg aacaaattat tgcccaaatt
     8161 gctgctgcaa ctcacagagg tgaataatgt tacccgtttt atggctacca tctgctcgtg
     8221 atgatttgcg ccagatcgta gcctacatag ctaaagaaaa tcccgcggca gcacgcagaa
     8281 tgaaaattcg aattgaaaca tccgttttgc cgctcacaga gcatccttac ctgtatcctc
     8341 caagcgagcg agttcttggt cttcgtgaaa ttgtaactca tcctaactac atcatccttt
     8401 accgagtaac agcatcaagc atagaagtag taaatattgt gcattctcga agacagtatc
     8461 caggcaaaaa cagttaatta ttcccgccga cagaaccacc ttagggtggt tttttcttgc
     8521 cccaacaaaa aacaatagcg atgctattta catcaaaaaa aataatgatg ctattgtttc
     8581 aggcgtcaac ccaccccgcc ccacagaatg cagggcaata cttcgagtta ccagacagtg
     8641 gtcaggggtt aagtagccag cccgaggcgt aagaacatga cggcagggtt caatctttaa
     8701 ctatgcagca ggttttttgt tccgccaccc cggcgttaag gggaaatgag gtcagcatgg
     8761 atactatcga tcttggcaac aacgaatctc tggtgtgcgg cgtgttccct aaccaggatg
     8821 gaacgtttac cgccatgacg tataccaaaa gcaaaacgtt taaaaccgaa tctggcgcgc
     8881 gttgctggtt agccagaaac actgactgat gaggctgacg atggaattta aagatttacc
     8941 gtctgatgta cagaaaacag cagctcatac attgcattct gtgctgcgag aaatcgggaa
     9001 agatattgca agcgagccag caaaagatct ggcccggaaa atcaagaccg ctttcgttga
     9061 gctttataat gttggcactg actctgaaac tgtcgagacc aagaccgtaa gttcaccaat
     9121 attctcactt gagccaagcg tattatcagg tgaaatatgc accgagattt ccagcgaact
     9181 gcttccggta atacgagaag caattcgccg tcgcggtttg gatggaagtt atgatcatga
     9241 cgttctgcaa gttctcagga caatggtgac tt
//
LOCUS       DAJVQG010000145         9217 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJVQG010000145 DAJVQG010000000
VERSION     DAJVQG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9217
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.145"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            73..732
                     /locus_tag="OQM83_003286"
     CDS             73..732
                     /locus_tag="OQM83_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCS1521477.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            836..1810
                     /gene="ghrB"
                     /locus_tag="OQM83_003287"
     CDS             836..1810
                     /gene="ghrB"
                     /locus_tag="OQM83_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCS1521478.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(1860..2570)
                     /locus_tag="OQM83_003288"
     CDS             complement(1860..2570)
                     /locus_tag="OQM83_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCS1521479.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            3004..3294
                     /locus_tag="OQM83_003289"
     CDS             3004..3294
                     /locus_tag="OQM83_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCS1521480.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            3575..3787
                     /gene="cspA"
                     /locus_tag="OQM83_003290"
     CDS             3575..3787
                     /gene="cspA"
                     /locus_tag="OQM83_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCS1521481.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="OQM83_003291"
     CDS             complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="OQM83_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCS1521482.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="OQM83_003292"
     CDS             complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="OQM83_003292"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCS1521483.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="OQM83_003293"
     CDS             complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="OQM83_003293"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCS1521484.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(7538..7837)
                     /locus_tag="OQM83_003294"
     CDS             complement(7538..7837)
                     /locus_tag="OQM83_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCS1521485.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            8012..9007
                     /gene="wecH"
                     /locus_tag="OQM83_003295"
     CDS             8012..9007
                     /gene="wecH"
                     /locus_tag="OQM83_003295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCS1521486.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(9049..>9217)
                     /locus_tag="OQM83_003296"
     CDS             complement(9049..>9217)
                     /locus_tag="OQM83_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521487.1"
                     /translation="MVGLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPK
                     ETQVTQEEYSE"
BASE COUNT         2287 a         2331 c         2268 g         2331 t
ORIGIN      
        1 tcatgttcac gcggcataat ctcccgccac ggaacccgtg gcaaagaata aaaaggttat
       61 taaggattaa caatgaagaa acgtgtttat cttattgccg ccgtagtgag tggcgctctg
      121 gcggtatctg gctgcacaac taacccttac accggcgaac gcgaagcagg caaatctgct
      181 atcggcgcag gtctgggctc tctcgtgggc gcgggtattg gtgcgctctc ttcttcgaag
      241 aaagatcgcg gtaaaggcgc gctgattggc gcagcagcag gcgcagctct gggcggcggc
      301 gtgggttatt acatggatgt gcaggaagcg aagctgcgtg acaaaatgcg cggcactggt
      361 gttagcgtaa cccgcagcgg ggataacatt atcctcaata tgccgaacaa tgtgaccttc
      421 gacagcagca gcgcgaccct gaaaccggca ggcgctaaca ccctgaccgg cgtggcaatg
      481 gtgctgacag agtatccgaa aacggcggtt aacgtgattg gttataccga cagcacgggg
      541 ggtcacgacc tgaacatgcg tctctcccag cagcgtgcgg attccgttgc cagcgcgttg
      601 atcacccagg gcgtggacgc cagccgcatc cgtactcagg gccttggccc ggctaaccca
      661 atcgccagca acagcaccgc agaaggtaag gcgcaaaacc gccgtgtaga aattacctta
      721 agcccgctgt aatccctttc atgccaggtg atgcagaaat cacctggcat tcttcagttt
      781 tccttcatca tatttcaggc taaggtgatc gccttatcag tgaatggaga gaagcatgaa
      841 gccgtccgtt atcctctaca aagccttacc tgatgattta ctgcaacgcc tgcaagagca
      901 tttcaccgtt caccaggtgg caaacctcag cccacaaacc gtcgaacaaa atgcagcaat
      961 ttttgccgaa gctgaaggtt tactgggttc aaacgagaat gttgatgccg cattgctgga
     1021 aaaaatgccg aaactgcatg ccacatcaac gatctccgtc ggctatgaca attttgatgt
     1081 cgatgcgctt accgcccgaa aaattctgct gatgcacacg ccaaccgtat taacagaaac
     1141 cgtcgccgat acgctgatgg cgctggtgtt gtctaccgct cgtcgggttg tggaggtagc
     1201 agaacgggta aaagcaggcg aatggaccgc gagcataggc ccggactggt acggcactga
     1261 cgttcaccat aaaacactgg gcattgtcgg gatgggacgg atcggtatgg cgctggcaca
     1321 acgtgcgcac tttggcttca acatgcccat cctctataac gcgcgccgcc accataaaga
     1381 agcagaagaa cgcttcaacg cccgctactg cgatttggat acactgttac aagagtcaga
     1441 tttcgtttgc ctgatcctgc cgttaactga tgagacgcat catctgtttg gcgcagaaca
     1501 attcgccaaa atgaaatcct ccgccatttt cattaatgcc ggacgtggcc cggtggttga
     1561 cgaaaatgca ctgatcgcag cattacagaa aggggaaatt cacgccgccg ggcttgatgt
     1621 cttcgaacaa gagccactgt ccgtagattc gccgttgctc tcaatggcca acgtcgtcgc
     1681 agtaccgcat attggatctg ccacccatga gacgcgttat ggcatggccg cctgtgccgt
     1741 ggataattta attgatgcgt tacaaggaaa ggttgagaag aactgtgtga atcctcacgt
     1801 cgcggactaa gccgcgactg cgtgaagtaa agcccgataa tcgctcgggc ttttactctt
     1861 tattgggttg cagtaactgc tgtagtccag gcctgattaa acgcctgatg ttgtgccggt
     1921 aatggcgcaa tcagtttgtt atattcactt gcctgctgtg aagtcgggaa ctggatgcca
     1981 tttgcgacaa agctcacctg agtaccttgc tgagcaatgt agtctccaac catcaccagt
     2041 tgttgcgtga aagtttgtgc cgccggaatc aacggttgca atgcatcagc tggcgtcgtt
     2101 accactttgg tgaatgcctg atcaaatacc ggtttcaggt catcgctctg ttttagcgca
     2161 gagtgcgcag catcggcctg caacttcgca ttttgcagtt gttgcgccag cacacccagc
     2221 gaaccattca tttcacgcag cggaccgctt tgcgtaacgt aatcctgcgg tacgcgaatt
     2281 gcgttaacgc tgtctacgac cgggcgcaga ccagaatcca tcgcctgatt cacctgctga
     2341 gaataaccat acagaatcgc gtaatcagag acaaaaggac caaactgttt tttctgatcg
     2401 gcagtcaggg ttggtagacg ttcaccgcta cgcatcactg tattctgcag gaagtcgata
     2461 aacgctttac gctgatcgcc ttctttatca aaacacccac tcaggctaac taccattaat
     2521 aacgccgcaa gaggcgcaaa ccagcgagag caggactttc ctgtcgccat tttcttactc
     2581 ctttcaccca aaaaaagcgc acaacgacac acgcgtgtct gacgtgacaa ggatagtcca
     2641 ggacaggctt gcaggatacc ctttctacgt aatcttcctg gtaaaatcat ggagaaaatg
     2701 aaacaaggtt aatttgttgt ttcattgtta aaaaatgata gtgccgttgc cttttttgta
     2761 gccagagatt tacgccttcg cagaaggtta gattttcgaa atctttacaa aacaggagtg
     2821 gtaagagata tatcggcatt tatcagatcg ttaggtggct ataagtcacg ctaaatgaca
     2881 ggctgaatcg aatcatagcc agagcatgcc ctgacttcac cccgctgtgt ctgcttttcc
     2941 cgactattct taatgagctt cgatgcaatt cacgatcccg cagtgtgatt tgaggagttt
     3001 tcaatggaat ataaagatcc aatgcatgag ctgttgagca gcctggaaca gattgttttt
     3061 aaagatgaaa cgcagaaaat taccctgacg cacagaacaa cgtcctgtac cgaaattgag
     3121 cagttacgaa aagggacagg gttaaaaatc gatgatttcg cccgggtttt gggcgtatca
     3181 gtcgccatgg taaaggaatg ggaatccaga cgcgtgaagc cttcaagtgc cgaactaaaa
     3241 ttgatgcgtt tgattcaagc caacccggca ttaagtaagc agttgatgga atagactttt
     3301 atccacttta ttgctgttta cggtcctgat gacaggaccg ttttccaacc gattaatcat
     3361 aaatatgaaa aataattgtt gcatcacccg ccaatgcgtg gcttaatgca catcaacggt
     3421 ttgacgtaca gaccattaaa gcagtgtagt aaggcaagtc ccttcaagag ttatcgttga
     3481 tacccctcgt agtgcacatt cctttaacgc ttcaaaatct gtaaagcacg ccatatcgcc
     3541 gaaaggcaca cttaattatt aaaggtaata cactatgtcc ggtaaaatga ctggtatcgt
     3601 aaaatggttc aacgctgaca aaggcttcgg cttcatcact cctgacgatg gctctaaaga
     3661 tgtgttcgta cacttctctg ctatccagaa cgatggttac aaatctctgg acgaaggtca
     3721 gaaagtgtcc ttcaccatcg aaagcggcgc taaaggcccg gcagctggta acgtaaccag
     3781 cctgtaatct ctgcttaaaa gcacagaatc taagatccct gccatttggc ggggattttt
     3841 ttatttgttt ttcaggaaat aaataatcga tcgcgtaata aaatttagta ttatttttgt
     3901 gaagaataaa tttgggtgca atgagaatgc gcaacgccgt aagaaaggcg ggaataactt
     3961 cccgccaaag actcttactc tttcaaattg caggctaaaa atgccgccag ctcataactc
     4021 tcctgtttaa tatgcaattc acacagtgaa tctcttatca tccaggtgaa aaataaaagc
     4081 gtgaaacaaa tcactattaa agaaagtaat ctatatttct gcggcattcc cagcctcctg
     4141 tgttgatttc caacgagtaa gaggctaacc ttatggtgtt caggcatagg gtagcctcac
     4201 tttgatttat agtcaggtgg ggcttttctc tgtctgcctt tcggtgaata cctgagacaa
     4261 acagtctcaa gcacccgtgg ctattctagc ttaataagtt tgtttcttct ccttgatata
     4321 atcctaaaaa aacctcataa aattaatata tgagataatc tttattcaac agaagattat
     4381 taaaggttgc tgtattattt agcgataata aaaaaagcct gccagatggc aggcttttta
     4441 ataacggcgt tattactgca acagcgaaat atccgcaacg cgcaggaaca gttcgcgcag
     4501 tttctccagc atggtcagac ggttgagacg caattctttg tcatcaacca tgaccatcac
     4561 tttatcgaag aaagcatcaa ccggctcacg cagctctgcc agttcgacca gcgcatcctg
     4621 gtaacgacct tccgcaaagt acggctccag cttgtcacgt agcaccacaa cctgcatcgc
     4681 cagtttaatt tcttccggct ctttcagggt agaggcattc acgcggtcgc tcagcacttc
     4741 gtcagatttc gccagaatgt tagatacacg tttgttcgcc gccgccagtg cagcagctgc
     4801 atccagggta cggaaatgcg ataccgcttt catacgggca tcgaaatcag ccggacgagt
     4861 cggacgacgc gccagtaccg cctggatggt gtcaacggtg taaccttcgt cctgatacca
     4921 ggcgcggaag cgaccgagca taaagtcgat aacatcatca actacgttgg cgttagtcag
     4981 cttatcgcca tacagacgca ctgcttcttc ggtcagcgtt tgcagatcaa ggttgaggtt
     5041 cttctcaacg ataattcgca gcacgccaag tgcggcacga cgcagcgcaa acgggtcttt
     5101 gtcgcctttc ggatgctgac cgataccgaa gatacccgcc agagtatcca tcttatcagc
     5161 aatcgccagc gcacaagcta ccgggttgga cggcaggtca tcaccggcaa agcgcggctg
     5221 atactgctca ttcagcgcga cggctacgtc ttccgcttcg ccatcgtgac gcgcgtagtg
     5281 catccccata acgccttggg tgtcggtgaa ctcgaagacc atgttggtca tcaggtcaca
     5341 cttagacagc aggcccgcac gggttgcgtg gttaacgtca gcgccaatct gttcagcaat
     5401 ccagccagcc agcgcctgga tgcggtcagt tttgtcacgc agcgtaccca gctgttgctg
     5461 gaacagcacg gtttgcaggc gcggcaggtt atcttccaga cgttttttac ggtcggtatt
     5521 gaagaagaac tcggcatccg ccagacgtgg acgaacgact ttctcgttac cagagataat
     5581 ttgctgcgga tctttcgatt cgatgttggc aacgaagatg aagttcggca gcagtttgcc
     5641 atcgttcgca tacaccggga agtatttctg gtcacctttc atggtgtaaa ccagggcttc
     5701 agccggcacc gcgaggaatt tctcttcgaa ttttgcggtc agaacgaccg gccactccac
     5761 cagcgaagcc acttcttcca gcaggctttc gcttaagtca gcgttaccgc caatcttacg
     5821 cgctgcttct tcggcatcgg ctttaatctt cgctttacgt tcttcgtaat cggcgatgac
     5881 tttcccgcgc tcacgcagaa tttccggata ctgatcggcg ttgtcgatgg tgaattccgg
     5941 ttcgcccata aagcggtggc cgcgaatcac gcgatcggac tgaatgccca gaatggttgc
     6001 cggaatgact ttgtcgccca gcagcagggt cacggtgtgc accggacgca cgaagtgcac
     6061 gtcgcttgcg ccccaacgca tcagtttcgg gatcggcagt ttcgccagcg aagtcgcaac
     6121 catattcggc agtagtgctt cagtgctttc gcccttcaca tgggcgcgat acagcagcca
     6181 ttcgccttta tcggtagtca gacgctcagc ctggtcaacg gtaataccgc aaccacgcgc
     6241 ccaaccttct gccgctttgc tcggtttgcc ttcagcgtcg aacgcctggg caatcgccgg
     6301 gccgcgtttt tcgatttcac gatctggttg cgcttccgcc aggttagcta ctttcaacgc
     6361 cagacgacgc ggagcagcaa accattgaac ggtgccgtgt gcgaggccag cgttatccag
     6421 ctccgcagta aagttcgcag caaaggactc agccaggctg cgcagtgctt ttggtggcag
     6481 ctcttcagtg ccgatttcca ccagaaaagt tttctcagac atagccgcct cttacttatc
     6541 tttgttgcac atcgggaagc cgagggcttc acgggaagcg tagtatgctt ctgccactgc
     6601 tttggtcagg gtgcgaatgc gcagaatgta gcgctgacgc tcagtgacgg agatggcttt
     6661 acgcgcatcc agcaggttga agctgtgggc ggctttcaga atacgctcgt aggctggcag
     6721 cggcagcgga ttttccagcg ccagcagctg ctgcgcttct ttctcgtact gctcgaagca
     6781 ggtgaacagg aaatccacat ccgcgtattc gaagttgtaa gtggactgct ccacttcgtt
     6841 ctgatggaac acgtcgccgt aggtggtttt acccagcggg ccgtcgctcc agaccaggtc
     6901 gtaaacgctg tctacgccct gaatgtacat ggccagacgt tccagaccgt aggtgatctc
     6961 gccggtaacc ggtttacact ccagaccacc aacctgctgg aagtaagtga actgcgtcac
     7021 ttccatgccg tttagccaca cttcccagcc cagtccccag gcacccagag tcgggttttc
     7081 ccagttatct tccacgaaac ggatgtcgtg aatagtcggg tccatgccca gctctttcag
     7141 agaaccgagg tacagctcct gaatattgtc cggcgatggc ttaatgacca cctggaactg
     7201 atagtagtgc tgtaaacggt tggggttttc gccgtagcga ccatcggtcg ggcgacgaga
     7261 aggctgaaca taagcagccg ccatcggttc tggccccagc gcgcgcagac aggtcattgg
     7321 gtgagaggtt cccgcgccga cttccatgtc caatggttga acaatggtgc agccctggcg
     7381 agcccagtaa tcctgtaagg tcaggatcaa gccctggaag gtcctggtat caaacttttg
     7441 catattattt cgtgctggat acgtgtggat ttaaaggaag ggatcagtat acccgctgga
     7501 tggaagatat acagtacgaa acgggaaaaa gcagggctta acgcatggaa agatgcaaaa
     7561 aatggccatc tgcgtcaaaa gaacagacaa actgctcttt acggaaggta taaccgcgca
     7621 tttcatagct tccctggaac tgttcgaagg cggtgacgtc gattttttgc tcgccggtgt
     7681 tataacgttt tgccgcctga tccttgcata acgcttccat attcaacgaa cgcagaggat
     7741 caacctttac ccgttgtgct ttctgcacgg gtgacggtgt agaccaaccc actagcacca
     7801 taagcgccat tgccggaaag aaagcgttca tcatcattaa attaccgtct ggtttgcagg
     7861 tgagtcttat tatttatatc ggtatagtgg ctgatatctt acgaatttcc ttaagcctct
     7921 ataagctgta gtgataaaac tcagattatt ccagtgcgcc aaccaaatga tttatcggtc
     7981 agactttttt taaccagata cagagacaca gatgcagccc aaaatttact ggattgataa
     8041 cctgcgaggg atagcgtgtt taatggtggt gatgattcac accactacct ggtatgtgac
     8101 caatgctcat agtgttagcc ccgtcacatg ggatatcgcc aatgttctga attctgcctc
     8161 tcgtgtcagc gtgccgctat ttttcatgat ttccggctat ctcttttttg gcgaacgcag
     8221 cgcccagccg cgccatttct tgcgtatcgg cttatgtctg attttttata gcgcaatcgc
     8281 actgctctac attgcgctgt ttacctccat caatatggag ttagcgctga aaaacctgct
     8341 gcaaaagcca gtgttttacc acttgtggtt tttcttcgcg attgcggtga tttatctggt
     8401 ttcaccgctg attcaggtga agaacgtcgg cggaaaaatg ttgctggtgc taatggcggt
     8461 gattggcatt atcgctaacc caaacacagt gccgcagaaa attgacggtt ttgaatggct
     8521 gccaattaac ttatatatca atggcgatac tttttactac attctgtatg gcatgttggg
     8581 ccgcgctata gggatgatgg acacacagca taaagcactg tcgtgggtga gcgccgcgct
     8641 gtttgcgacg ggggttttta ttatctctcg cgggacatta tatgaattgc agtggcgcgg
     8701 aaattttgcc gatacctggt atctttactg tgggccgatg gtttttatct gcgcaatcgc
     8761 gctattgact ctggttaaaa acacgctgga tacgcgtacc attcgcggac ttggcttaat
     8821 ctcccgccat tcattgggta tatacggatt ccacgccttg attatccatg cgctgcgcac
     8881 ccggggaatt gagcttaaaa attggccaat actggatatt atttggatct tttgcgcgac
     8941 gttggcagcg agtttgttac tttctatgct ggtacaacga atcgacagaa acagattagt
     9001 gagttaagta aaagcccgtt cacattggac tgaccgggct tacgtgaatt attcgctgta
     9061 ttcttcctgg gtaacctgtg tttcttttgg cgggtttatt ccggcatcac gaatattctt
     9121 ctgcaccgct actgcaccaa acaagcttaa gaagaaagcc agtccataaa aacctttttc
     9181 actgagtaat aatgtcgcat tccacagacc taccatc
//
LOCUS       DAJVQG010000146         9188 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJVQG010000146 DAJVQG010000000
VERSION     DAJVQG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9188
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.146"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            171..1769
                     /gene="rtcR"
                     /locus_tag="OQM83_003297"
                     /pseudo
     CDS             171..1769
                     /gene="rtcR"
                     /locus_tag="OQM83_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1751..2509)
                     /locus_tag="OQM83_003298"
     CDS             complement(1751..2509)
                     /locus_tag="OQM83_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCS1521488.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="OQM83_003299"
     CDS             complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="OQM83_003299"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCS1521489.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="OQM83_003300"
     CDS             complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="OQM83_003300"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCS1521490.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3917..5422
                     /gene="glpD"
                     /locus_tag="OQM83_003301"
     CDS             3917..5422
                     /gene="glpD"
                     /locus_tag="OQM83_003301"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCS1521491.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5474..6079)
                     /locus_tag="OQM83_003302"
     CDS             complement(5474..6079)
                     /locus_tag="OQM83_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521492.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6082..6849)
                     /locus_tag="OQM83_003303"
     CDS             complement(6082..6849)
                     /locus_tag="OQM83_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521493.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6852..7562)
                     /locus_tag="OQM83_003304"
     CDS             complement(6852..7562)
                     /locus_tag="OQM83_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521494.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7578..9083)
                     /locus_tag="OQM83_003305"
     CDS             complement(7578..9083)
                     /locus_tag="OQM83_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCS1521495.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENIR
                     NGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT         2372 a         2358 c         2261 g         2197 t
ORIGIN      
        1 ttgtttgctg tcgtgataat tgcaaccgtc gtgccagaaa attaaataag cagattaatt
       61 ttttaattca ttgtttttaa ataggttaaa tctttacgtc cgtaaacgga gattttccac
      121 aaaaccaatt caccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa
      181 cagtggcttt tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct
      241 ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc
      301 tggaattgtt gcacgacact cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg
      361 ccagcgtttc gccagaaaca gaagtcgtgg gcgttgagat tgaactgcat aacccgtggg
      421 atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag
      481 aaaaagaaga ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt
      541 ttctgctggc ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca
      601 aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt
      661 ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt
      721 ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg
      781 cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc
      841 tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg
      901 aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa
      961 aaggcgcgtt taccggggcg cggtaatctc gtgaaggttt attacgcagc gccaacggcg
     1021 gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaggca atgctgctaa
     1081 aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt
     1141 ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
     1201 gcgaagatct gtacgcgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc
     1261 gccaggaaga tattgaaccg aaccttgatt atgaagtgga gcgccacgcc acactcactg
     1321 gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc
     1381 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
     1441 cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc
     1501 gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag
     1561 aaaacatcaa tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgctagg
     1621 caaagtcgct ttctgcagcc ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca
     1681 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag
     1741 ccgtgcagga tcagcacagc tccagttgaa tatgatggtc cgtcagcacc tgcattacgc
     1801 ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga
     1861 ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct
     1921 caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc
     1981 tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga
     2041 gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa
     2101 tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac
     2161 gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
     2221 taaacagtgt cgagccgttg gggatttgct ccgccacttt gcgggcaatc ctctcttttt
     2281 cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg
     2341 ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc
     2401 gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct
     2461 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc
     2521 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
     2581 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
     2641 gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
     2701 ggatcgcgtt caccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
     2761 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
     2821 agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
     2881 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
     2941 aagtaacgcc agaactcaaa tttcagtgct ggatcgaatg gccaggccag ccataacatc
     3001 acttcctgat cgccgagaat ttgcatggca ataaacacca ccacgcaggc gatcatcatc
     3061 acccaggtca ccggacctgc gcgttcacgc aaggcggcaa agaaaggata acggcgataa
     3121 tgcaggccac tgccggtatg gcctgcctgc cagctggccg ccagataacg cggatctgcc
     3181 gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct cggcctggga ctcatccgcc
     3241 agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg
     3301 taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt
     3361 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
     3421 ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc aatgctatag accacatcgt
     3481 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
     3541 ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta
     3601 aatggaaagc ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca
     3661 gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaact
     3721 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc
     3781 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
     3841 acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa
     3901 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg
     3961 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
     4021 ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
     4081 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct
     4141 gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct
     4201 gcgcccggcg tggatgattc gcattggtct gtttatgtac gatcatctgg gtaaacgcac
     4261 cagcctgccg ggatcaactg gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat
     4321 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
     4381 cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc
     4441 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
     4501 tagctggcaa gcgcgcggct tggttaacgc caccggcccg tgggtgaaac agttcttcga
     4561 cgacaggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
     4621 ggtgccgcgc gtgcataccc aaaagcaggc ctacattctg caaaacgaag ataaacgcat
     4681 tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata
     4741 caaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaattacc tgctgaaagt
     4801 gtataacacg cactttaaaa agcagttaag ccgtgacgat atcgtctgga cctactccgg
     4861 tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac
     4921 ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagtt
     4981 gaccacctac cgcaaactgg cggaacatgc gctggaaaaa ctgacgccgt attatcaggg
     5041 cattggcccg gcatggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
     5101 cgacgattac gcagctcgtc tgcgccgtcg ctatccattc ctgactgaat cgctggcgcg
     5161 tcattacgca cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat
     5221 aagcgacctc ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt
     5281 ggatcacgaa tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa aacaaggcat
     5341 gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca
     5401 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga
     5461 ttccttagac tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac
     5521 ttaagcacgt tacccaatcg ccccacttct tcctctggtt tctatttttt ctgcaccaat
     5581 ggcaataata tcccaatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc
     5641 aaggctgcgg ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat
     5701 ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga
     5761 gaattaacca gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt
     5821 caggaggcgt tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg
     5881 catattcaac cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg
     5941 gaaaggagaa tattatttct gccagaaaag ttaacgtgaa tataagtacg gctaagacaa
     6001 taaccgtcgg ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat
     6061 aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc
     6121 gcataaacac tcatcaatta tccagtcatc ttcccagacc tgatattctg tcattggttc
     6181 gggctgtttt agcgggttct caatcgcttg ccgcgctctt tctttcagta aaggatgatt
     6241 atttctagcc caggcattga tttgggtggt aatggcgtta tcgatatcgt cctggttatc
     6301 tctgatataa tcctcacaat atctgttcaa taaacaggca agagaaacgg tcgaccaaaa
     6361 gccgggaact gaattaatgg catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa
     6421 catttccagc atatactgta attcgacagc ttcatcgccg gatacccaat cagtcatata
     6481 ctggcaggca aatcgctgta aaattgcccg ttcatccgca cgccagtttt ctttgttggc
     6541 aaggttgata cggcgcagag aaaaaactac gtgaatattg tcaaaatgcc cctgtgcaac
     6601 aaattccagt aaacgcggca gaaaatagcg gacctcatcg ctattttgcg gatcaggccc
     6661 tgggctacta ttccaggcat tgattaagga atacgggatc gctcgtaacg atgtactccg
     6721 taatgccttt tgttcctgct gactcaggca atattcacaa caaaccacaa atttctgcgg
     6781 cacccgataa cgacgaaagg tgtgataaat ctgctcaatt aatgcctgca tggcggggct
     6841 gggttgcata gttaccccct cgcccgctga tcgcgtgtaa acggttgata gacaaaacga
     6901 gcattcgccg cgttcaggcg aataagggcg ctacgcgaaa gggaaaagct ggggcgaatg
     6961 caactattgc agtctcgaca gctttcctct gcgcaatttt ccactgagta atgacgcgtg
     7021 tagagtttca gttcggcctg cttatcgcga aatccgcgta aactgttatt gttatgaaat
     7081 ttccaggcat ctttaaagac ctgaaagata tagacatcct caatccagga gtcacatttt
     7141 ttaagcgggc gctttgctat tatcacccgc tcatccggat ttagcatcgc cgtaaattgt
     7201 acggtgacgt catttgtcgt gttcaggtca cattgaacat ctggtacatc actgatatag
     7261 gggtcaaatc caaaatcagc accacaggca caaagacggc taatggtggc ggtatcgagg
     7321 gaaaatcccg gccatacctt gctggaggtt aagccaatat ttaatgtgac atcatatcga
     7381 agcaatccct caagaccgtc agcaccgacg aacttttccc cttcatccaa ggcctggtta
     7441 ataagttgct gaacaatgcc ttcatcccag tatgccgatt tgcagttggc aatcgtaata
     7501 tccacgcctg tgaagggatc agtaccggca aggcttgcaa ataatgtatt aactaattcc
     7561 atttttatta tccagcttta aggtagcgtc ttatacatcg ggacataacc tacatcttcc
     7621 accaaactct gtccctgcgg cgttaaaaac cactccaaca atttttgtgt ctctgacgtc
     7681 gtattttccc gcgttaccat aaaggcatcg acgatatagg gatatttgcc gttgcgaatg
     7741 ttttcggcag tcggtgcaat accgttaatc gctagcagtt ttatattttt atcggcgttc
     7801 atttgtgttg cgtaataacg gaaggtgtaa cctattgcgt tgtttgtatt acggtattcg
     7861 gcaacgactt taatcattcc ctccatcata cttgccactt ctgtttcctg cggcgagatc
     7921 atgcggacat ttttcatgac ctgggattgc atcaccgtct ggctaccaga gtcttccggg
     7981 cgttgccagg tctggatctc ctgatcgtta ccgccaacag tacgccagtt ggtaattgca
     8041 ccactgaaga tgtcacgcac ctgttgttca gtcagggaat taaccggatt atccgcattg
     8101 acgatgaaaa caaacgcttc acgggcaaaa ggcgtgtaca gcaaagtaac gcccgattcc
     8161 tcagcgcgtt ttttctgccc accggaaggt tgcgccacaa aaataatatc agcatcacct
     8221 ttaacaatcc tgttatacgc atcgggggta cgagagctct ccagatattc cctggtgtga
     8281 aaatcttccg gtataacgct taaagcataa aatgcagagg cataaattgg gtacgccgcc
     8341 gtagcaccat cgagtcgcgg ccagttttgc gtaaactgaa tttgcggttt accacgcagt
     8401 ggtgtcagtt gattattgag tttatcgggt cgccaggccc aggtatcaat attttcgcgc
     8461 acaccgacac cgggattgag ctgtttatat aaataaccct gataaccagc gacaaaacca
     8521 agcatgagaa ggataaacag gagggaatta cggcaacgta gcccggatgt accggttaaa
     8581 ggatgtctgg ttggccggcc gtaccccagc gaaaaactga tatgcgcgcc aacgggtata
     8641 atgactatcc accagtactc acctccgact actgagacta acaaaacgcc cagccagggg
     8701 agcataagat cagccaacca acccgatccc atctggtgaa attgataatc tgccagatgc
     8761 agcgcgatta tccagccaac caggattaat aaaccgggta ctaaaaaaag acattgtcgc
     8821 cagacggcta tttctctttt ttctggtgca ctatcgcctg cccatactct ccccgagaaa
     8881 aatagcatta atgcgcccag cgccagcata aaaatgttga gcagaaggta acttgttggc
     8941 gtgataagga tattacaaac ttcaataata ccggcaagtc cgacacccag catagcaaca
     9001 accgccgcca aaaattgcgt cagtatgggg atgccgaaaa aagtcattac cagcgaggac
     9061 aaaatccatt ttctgttttg cattattctt tccattcttt ttgaatggtg aaattatact
     9121 ccccgagtcc ccttgcccct tctggacact tttccgaaat gatggcggaa aaaaacggga
     9181 ccctttgg
//
LOCUS       DAJVQG010000147         9162 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJVQG010000147 DAJVQG010000000
VERSION     DAJVQG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9162
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.147"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            200..1066
                     /gene="folD"
                     /locus_tag="OQM83_003306"
     CDS             200..1066
                     /gene="folD"
                     /locus_tag="OQM83_003306"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCS1521496.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1068..1280
                     /gene="ybcJ"
                     /locus_tag="OQM83_003307"
     CDS             1068..1280
                     /gene="ybcJ"
                     /locus_tag="OQM83_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCS1521497.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1388..1909
                     /locus_tag="OQM83_003308"
                     /pseudo
     CDS             1388..1909
                     /locus_tag="OQM83_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
     gene            complement(1945..3330)
                     /gene="cysS"
                     /locus_tag="OQM83_003309"
     CDS             complement(1945..3330)
                     /gene="cysS"
                     /locus_tag="OQM83_003309"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCS1521498.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3504..3998
                     /gene="ppiB"
                     /locus_tag="OQM83_003310"
     CDS             3504..3998
                     /gene="ppiB"
                     /locus_tag="OQM83_003310"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCS1521499.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4001..4723
                     /gene="lpxH"
                     /locus_tag="OQM83_003311"
     CDS             4001..4723
                     /gene="lpxH"
                     /locus_tag="OQM83_003311"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCS1521500.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGCRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4841..5350
                     /gene="purE"
                     /locus_tag="OQM83_003312"
     CDS             4841..5350
                     /gene="purE"
                     /locus_tag="OQM83_003312"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCS1521501.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVLHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5347..6414
                     /gene="purK"
                     /locus_tag="OQM83_003313"
     CDS             5347..6414
                     /gene="purK"
                     /locus_tag="OQM83_003313"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCS1521502.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6551..7444)
                     /locus_tag="OQM83_003314"
     CDS             complement(6551..7444)
                     /locus_tag="OQM83_003314"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCS1521503.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7441..8094)
                     /locus_tag="OQM83_003315"
     CDS             complement(7441..8094)
                     /locus_tag="OQM83_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCS1521504.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8105..>9162)
                     /locus_tag="OQM83_003316"
     CDS             complement(8105..>9162)
                     /locus_tag="OQM83_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCS1521505.1"
                     /translation="EGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMGGITSASMPML
                     VVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLSAALGRMERGI
                     DLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEVMDFLSVTDQF
                     FLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFTAPVNTPQGLF
                     FTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAARAVSEEMAEIY
                     LERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQIGAGTVRAPL
                     ACFEQALEALAESMGIG"
BASE COUNT         2038 a         2598 c         2429 g         2097 t
ORIGIN      
        1 aaggaaataa gtcagttcca gatttacaac gccatcatgg acgaaaaatg aagctttcaa
       61 tctcagcgac ggtgcgcccc accttcgcaa gaggtcgctt cacgcgataa atctgaaacg
      121 aaacctgaca gcgcgccccg cttctgacaa aataggcgca tccccttcga cctacgtaac
      181 agatggaatc ctctctctga tggcagcaaa gattattgac ggtaaaacga ttgcgcagca
      241 ggtgcgctct gaagttgctc aaaaagttca ggcgcgtatt gcagccggac tgcgggcacc
      301 aggactggcc gttgtgctgg tgggtagtaa ccctgcatcg caaatttatg tcgcaagcaa
      361 acgcaaggct tgtgaagaag tcgggttcgt ctcccgctct tatgacctcc cggaaaccac
      421 cagcgaagcg gagctgttgg agcttatcga tgcgctgaat gccgacaaca ccatcgatgg
      481 cattctggtt caactgccgt taccggcggg tattgataac gtcaaagtgc tggaacgtat
      541 tcacccggac aaagacgtag acggtttcca tccttataac gtcggtcgcc tgtgccagcg
      601 cgcgccgcgt ctgcgcccct gcacccctcg cggcatcgtc acgctgcttg agcgttacaa
      661 cattgatacc ttcggcctca acgccgtggt gattggcgca tcgaatatcg tcggtcgtcc
      721 aatgagcatg gaactgttgc tcgcaggttg caccactacc gtgactcacc gcttcactaa
      781 aaatctgcgt caccacgtag aaaatgccga tctactgatc gttgccgtcg gcaagccggg
      841 ctttattcct ggtgactgga tcaaagaagg cgcaattgtg attgatgtcg gcatcaaccg
      901 tctggaaaat ggcaaagttg tgggcgacgt cgtgtttgaa gacgcggcta aacgcgcctc
      961 atacattacg cctgttccgg gcggcgttgg cccgatgacg gttgccacgc tgattgaaaa
     1021 cacgctacag gcgtgcgttg aatatcatga tccacagggt gagtaacatg gcgacatttt
     1081 ctttaggtaa acatccgcac gttgagctgt gcgacttgct gaaactggaa ggctggagcg
     1141 aaagcggcgc gcaggcgaaa atcgcgattg ccgaaggcca ggtgaaagcc gacggcgcgg
     1201 ttgaaacgcg caaacgctgc aaaatcgtcg ccggtcagac agtgagtttt gcaggtcaca
     1261 gcgtacaggt tgttgcctga tccctttccc ggcctgtatc aacaggccgg ttctcttttc
     1321 agacttttgc cctctacgcc ccgttcatcc atgctgatag catcgacatt ttgccgaagc
     1381 gaccatcatg ccaaccgtta ttacgcacgc cgccgttccc ctatgcattg gtttagggtt
     1441 gagttcgaaa gtcatccctc cgcgtctgtt atttgcctga atcatcctgg cgatgctgcc
     1501 cgacgccgac gtattgtcgt ttaaatttgg cgttgcttac ggcaatgttt ttggtcatcg
     1561 cgggtttacc cattcgctgg tgtttgcgtt tgttgtcccg ctgttatgtg tgctgattgg
     1621 acgccgatgg ttcagggctg ggctgattcg ctgctggctg tttttaaccg tctcgttgct
     1681 atcgcacagc atgctggatt cggtaaccac tggcggtaag ggtgttggct ggctgtggcc
     1741 gtggtcagat gaacgctttt tcgctccctg gcaggtgatt aaagtcgcgc cgtttgcatt
     1801 gtcttgttac accacgccgt acgggcatca ggtgattatt tctgaattga tgtgggtgtg
     1861 gttgccgggg atgctactga tgggaatgtt gtggtggcgc agacgataac cggatgcgaa
     1921 aactcgcatc cggcaatagc gcaattactt acgacgccag gtggtccctt gcgggccatc
     1981 ttccagaacg atccccatct cgttaagacg gtcacgcgcc gcatctgccg ccgcccagtc
     2041 tttcgcttta cgggcatcca gacgctgttg aattaacgct tcaatctcag ccacttcgct
     2101 gtcgtctgcc tgcgcgccgc tttgcaggaa cgcttccggt tcttgctcca gcaggcctaa
     2161 tacagcggaa agtttacgca ggtgagatgc cattgcattc gccgctgcca tatcttctgc
     2221 tttcagacgg tttacttcac gcgccatatc aaacagtacg gaataggctt ccggggtgtt
     2281 gaaatcgtcg tccatcgctt caataaagcg cgcttcaaac gcttcgccac cggcaggagc
     2341 aacggttttt tctgtgccgc gcagggcagt gtagagacgc tccagcgccg cacgcgcctg
     2401 cttcaggttc tcttcgctgt agttcagctg gctgcgatag tggcccgaca tcaggaagta
     2461 acgcacggtt tcagcatcgt agtatttcag cacatcgcgc acggtaaaga agttacccag
     2521 cgatttggac atcttctcgc ggtcaaccat caccattccg gagtgcatcc agtagttaac
     2581 atactgacca tcatgggcac aggtggactg cgcgatttca ttctcgtggt gcgggaacat
     2641 caggtcagaa ccgccgccgt ggatatcaaa gtggttaccc agctgcttgc agttcattgc
     2701 cgaacattca atgtgccagc ctggacggcc cgcgccccac ggagacggcc agctcggttc
     2761 gccctctttc gacatcttcc acagaacgaa gtccatcggg ttgcgtttgt cgtcgaccac
     2821 gtcaacgcgc gcgcctgcct gcagctggtc gagatcctga cgcgacagca caccataagt
     2881 cggatcggtc gggacgtcga acatcacgtc gccgttgtcc gccgcataag cgtgaccttt
     2941 ggcgatcagt tgttcagtga gttcaataat ttctgcgata tggtgcgtcg cgcgcggctc
     3001 catatccggg cgcagaatgt tcaaagcatc aaaatctttg tgcatttcgg cgatcatgcg
     3061 atccaccagc gccacaaagc tttcgccatt ttcattggca cgtttgatga ttttgtcgtc
     3121 gatatcggta atgttgcgca catacttcag tttatagccg aggaaacgca gatagcgcgc
     3181 aaccacgtca aaagcaacaa aggtacgccc gtgaccaata tgacagagat cgtaaacggt
     3241 gattccacac acatacatgc cgacttcccc ggcgtgaata ggcttaaatt cctctttttg
     3301 gcgtgtcaga gtattgaaga tttttagcat cgaagattcc gtttagacat gtgtgggtaa
     3361 ttgagttgcg tataataccc atatttcccg cgcgaatcag catacattgc gtgatgatcc
     3421 gatcgtgcgg ttatgctata acaccaccct atatatgacc cgaactgggt tgaagcacca
     3481 atcaaacgga acaggatgca aaaatggtta ctttccacac caatcacggc gatattgtca
     3541 tcaaaacttt tgacgataaa gcacctgaaa cagttaaaaa cttcctggac tactgccgcg
     3601 aaggttttta caacaacacc attttccacc gtgttatcaa cggctttatg attcagggcg
     3661 gcggttttga accgggcatg aaacaaaaag ccaccaaaga accgatcaaa aacgaagcca
     3721 acaacggtct gaaaaatacc cgtggtacgc tggcaatggc ccgtactcag gctccgcact
     3781 ctgcaactgc acagttcttc atcaacgtgg ttgataacga cttcctgaac ttctctggcg
     3841 aaagcctgca aggttggggc tactgcgtgt ttgctgaagt ggttgaaggc atggacgtgg
     3901 tagacaaaat caaaggtgtt gcaaccggtc gtagcggtat gcaccaggac gtgccaaaag
     3961 aagacgttat cattgaaagc gtgaccgtta gcgagtaatc gtggcgacac tctttattgc
     4021 agatcttcat ctctgcgtgg aagaaccggc gatcaccgcc ggttttctgc gttttttagc
     4081 gggggaagct cgcaaggccg acgcgctgta tattcttggc gatctgtttg aagcgtggat
     4141 tggcgacgac gagcccaatc cactccatcg ccagatggcg gcagcgatca aagcggtgtc
     4201 cgattctggc gttccctgtt atttcattca tggcaaccgt gattttctgc tcggcaaacg
     4261 ctttgcccgt gaaagtggca tgacgttatt gccggaagaa aaggtgctcg aactttatgg
     4321 ctgccgggtg ttgattatgc atggcgacac gctgtgcacc gatgacgcgg gttatcaggc
     4381 ttttcgcgcc aaagtccaca aaccctggct gcaaatgcta ttcctcgccc tgccgttgtt
     4441 tgtgcgcaaa cgcattgccg cgcgaatgcg agcgaacagt aaagaagcca acagcagtaa
     4501 atcgctggcg atcatggacg ttaaccaaaa cgcggtagtc agtgcgatgg aaaaacatca
     4561 ggtgcaatgg ctgatccacg gacataccca tcgcccggcg gtacatgaac ttatcgccaa
     4621 ccagcaacct gcttttcgcg tggtactcgg tgcctggcat acggaaggtt cgatggtgaa
     4681 agtcacggcg gatgacgttg agctgattca ttttccgttt taaaaaaccc gcaactttgc
     4741 tgatttcaca gccacgcaac cgttttcctt gctctctttc cgtgctattc tctgtgccct
     4801 ctaaagccga gagttgtgca ccacaggagt tttaagacgc atgtcttccc gcaataatcc
     4861 ggcgcgtgtc gccatcgtga tggggtccaa aagcgactgg gctaccatgc agttcgccgc
     4921 cgaaatcttc gaaatcctga atgtcctgca ccacgttgaa gtggtttctg ctcaccgcac
     4981 ccccgataaa ctgttcagct tcgccgaaag cgccgaagag aacggttatc aggtgattat
     5041 tgcgggcgca ggtggcgcag cgcacctgcc aggcatgatt gccgccaaaa cgctggtgcc
     5101 ggtgctgggc gtgccagtac agagcgccgc actgagcggt gtcgatagcc tctactccat
     5161 cgtacaaatg ccgcgcggca ttccggtggg tacgctggcg attggtaaag ctggcgcggc
     5221 aaacgcggca ttactggcag cacaaattct cgcgactcac gataaagagc tacaccagcg
     5281 tctgaatgac tggcgcaaag cacaaactga tgaagtgctg gaaaacccgg acccgcgagg
     5341 tacggcatga aacaggtttg cgtcctcggt aacgggcagt taggccgtat gctgcgtcag
     5401 gcaggcgaac cgttaggcat tgctgtctgg ccagtcgggc tggacgctga accggcggcg
     5461 gtgccttttc aacaaagcgt gattaccgct gagatcgaac gctggccgga aaccgcatta
     5521 acccgcgagc tggcgcgcca tccggccttt gtgaaccgcg atgtgttccc gattattgct
     5581 gaccgtctga ctcagaagca gcttttcgat aagctccacc tgccgaccgc gccgtggcag
     5641 ttacttgccg atcgcagcga gtggcctgcg gtgtttgatc gtttaggtga actggcgatt
     5701 gttaagcgtc gcactggtgg ctatgacggt cgcggtcaat ggcgtttacg cgccaatgaa
     5761 accgaacagt taccggcaga gtgttacggc gaatgtattg tcgagcaggg cattaacttc
     5821 tctggtgaag tgtcgctggt tggcgcgcgc ggctttgatg gcagcaccgt gttttatacg
     5881 ctgacgcata acctgcatca ggacggtatt ttgcgcacca gcgtcgcttt tccgcaggcc
     5941 aacgcacagc agcaggcgca agccgaagag atgctgtcgg cgattatgca ggagctgggc
     6001 tatgtgggcg tgatggcgat ggagtgtttt gtcaccccgc aaggtctgct gatcaacgaa
     6061 ctggctccgc gtgtgcataa cagcggtcac tggacacaaa atggtgccag catcagccag
     6121 tttgagctgc atctgcgggc gattaccgat ctgccgttac cgcaaccagt agtgaataat
     6181 ccgtcggtga tgatcaatct gattggtagc gatgtgaatt atgactggct gaaattgccg
     6241 ctggtgcatc tgcactggta cgacaaagaa gtccgtccgg ggcgtaaagt ggggcatctg
     6301 aatttgaccg acagcgacac atcgcgtctg actgcgacgc tggaagcctt aatcccgctg
     6361 ctgccgccag agtatgccag cggcgtgatt tgggcgcaga gtaagttcag ttaagcaggt
     6421 gctcatttga ttgccggatg cggcgtaaac gccttatccg gcttaccgct ccggtaccca
     6481 ttgtaggcct gataagacgc gtcaagcgtc gcatcaggca tggtgcaccg attgccggat
     6541 gctctcatca ctacagcgaa atacaggtcc ccgcttcgcc cgccagcgtc tcttcaattc
     6601 gcgataacgc cccaatccac gcgggtttac cacggcttct gacataacca ctcaccgccg
     6661 ttaccttcgg ccccatcgaa ccatcggctt tggcaaatgg cgctaactca tccggtgtgg
     6721 catggcgaat ggcacgttgc tgcggcgttc cccagttttc atataccgca tcagcatcgg
     6781 tgaggatcac cagtccatcg gcattaatct gctcggcgag caacgcagcg gcgagatctt
     6841 tatcaatcac tgcttcactc cctgccccat cttccgtcac aggcacacct ccgccgccac
     6901 tgcaaatcac cacatgcccc tctttgagca acaactcgat ggcttcgctg tcgagaattt
     6961 tacgcggttg cggagacgcc accacccggc gcaaatattt accatcacgt ttcatctgcc
     7021 agccgtaagc cgcttccagt gcctcttgtt cttctggctg ataaaccgga ccaataaatt
     7081 tctctggctg caaaaacgcc ggatcatcag gcgaaacctc aatgcgcgtc agcaccgtcg
     7141 taacaggcgg catctgcggc tgtgcgctca agctctgcgc cagcatatag ccaatcatcc
     7201 cctggctttc cgccaccagc acatccagcg gatacggttc tacctctttc cacgccagat
     7261 tctgtaacgc cagcagcccc acctgcggcc cgttgccgtg aacaatcgcc agccgataag
     7321 aacgggccag gcgtgccagc gcgggtacag cactggcgat attgcgatat tgattttccg
     7381 ccgtcagcgc ctcaccgcgc tggagtaagg cgttgccccc aagagctaca accagtgttt
     7441 tcatggtttt ccttgtaata attgttggcc aagccagaac cccagcagcg tatctgcccc
     7501 tgacgtatgc cccagcgcca gcagcgaatc aatcgcaaca gcggtacgtt tcgggcaact
     7561 cagagcatgg acaaagtgca ggagtggcga ggcgaaatat ccttgcgcgg cataacgtaa
     7621 ataactgacg ctcaccgctg tggtaacgag ttgaagattg tcggaatagg caaaaaactg
     7681 gcgtaactca gcaggcagcg gatcgcctgc cgccaacgcc agtggcccga aaagcccggt
     7741 ttcctccgcg cgttgcatcc atgcagctgc aagcggttga ggatgcgcag gcggcgtaat
     7801 acgcagcaaa caataacgct gtggctgttt aaccgtgaaa cgcccgaggc gaatcccttg
     7861 agccacaacc tgtggtcgtt cattgccgca taatccaccg cataacgcat cgaattgcgc
     7921 gcggcgaagc acccatcctc cggggccgaa accactaccc tgacgatgca gcgttaacag
     7981 ttcgccgctt tccgtcatca ggttaatcgc ccgtcgccac actcccgcta accgccagga
     8041 ctggcgataa ttcggtgcac tacggctggc aagcagagga tggatgatcg tcattgcgcg
     8101 ttcctcaacc aatccccatg ctttcagcca gtgcttccag cgcctgttca aaacacgcca
     8161 gcggtgcccg cacagtgcct gcgccaatct gcccgatccc cgcctcttta tgggcgatac
     8221 cggtattgat gagtggcgta atgtcggttt ctaccacgcg acgaatgtcc agtcccaggc
     8281 acgcgccctg aaaatcccag cctgggatct gcaactgcat attgcgctca aggtaaattt
     8341 ccgccatctc ttcagatacc gctctggccg cttccatgcc acccgcaccg acaaagcgcg
     8401 ttacgccagg cgctgcgatc attgccgcac ctccgatacc aaaggtttcg gtaatcgcgc
     8461 tatcgcccat atccgggttc gcctgctcct gcgagaagcc ggtgaaaaac agaccttgcg
     8521 gagtgtttac aggcgcagta aaccagcgtt cgcccagccc gcttacccga atcccgaaca
     8581 tattgccgtt gcgggtcatt gccgtgacga tgctgcctgc gcggatcatc gcgccagcat
     8641 ccatcgccgc cttgcagtaa gccatcgcga ggttgaggaa gaactgatcg gtcacgctga
     8701 ggaaatccat cacttcggcg atgtgctgtt tatcatgatc gaggcgagca atttgtgggg
     8761 ccagcgcacg cattaacagt gcagaggaag caatattgcg ttgatggaac tcatcgccca
     8821 tcgtaatgcc ctgcgccatc atcgccgtga gatcgatacc gcgctccatg cgccccagcg
     8881 ccgcgcttaa tactggcatt aacacatcgc gcatccagcg atggcgagtc aggacatctt
     8941 cgccgtaagc gccaaaacgc atcactttgc cgataccttc gttgaggttg cagtacgccc
     9001 ggttgccgtc ggtcacgttc tcgaccacca gcatcggcat actggcagaa gtaataccgc
     9061 ccattggacc gacggcattc acatggtgac aaggaatgaa gttcacttcc ccctgctcca
     9121 gtattgccag cgcctgcgct tcatctttcg cccagccttc ga
//
LOCUS       DAJVQG010000148         9113 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJVQG010000148 DAJVQG010000000
VERSION     DAJVQG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9113
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.148"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(350..1603)
                     /locus_tag="OQM83_003317"
     CDS             complement(350..1603)
                     /locus_tag="OQM83_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1521506.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1710..2603
                     /locus_tag="OQM83_003318"
     CDS             1710..2603
                     /locus_tag="OQM83_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1521507.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            2738..3958
                     /gene="mlc"
                     /locus_tag="OQM83_003319"
                     /gene_synonym="dgsA"
     CDS             2738..3958
                     /gene="mlc"
                     /locus_tag="OQM83_003319"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCS1521508.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            4083..4778
                     /gene="bioD"
                     /locus_tag="OQM83_003320"
     CDS             4083..4778
                     /gene="bioD"
                     /locus_tag="OQM83_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCS1521509.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="OQM83_003321"
     CDS             complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="OQM83_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCS1521510.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQASDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="OQM83_003322"
     CDS             complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="OQM83_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCS1521511.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6839..7693)
                     /locus_tag="OQM83_003323"
     CDS             complement(6839..7693)
                     /locus_tag="OQM83_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCS1521512.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="OQM83_003324"
     CDS             complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="OQM83_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCS1521513.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(8323..>9113)
                     /locus_tag="OQM83_003325"
     CDS             complement(8323..>9113)
                     /locus_tag="OQM83_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HCS1521514.1"
                     /translation="TSAKFERKPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHA
                     KTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSER
                     LAKIADTWELKKDEVIHPLPAYTPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNID
                     VLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGA
                     WLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSHSNLVQIEKV"
BASE COUNT         2380 a         2310 c         2368 g         2055 t
ORIGIN      
        1 cagacttccg tgagtggttg gtttcagcgt gtaacgatat gtacaaacgc tgaataaatt
       61 acagcattga taataacgta ttgtgactta agggaaattt agctaccaat aatagtagtc
      121 ttgatcgggt taactatttt actacttaca aagcggtaga ataactgcgc atcagtaata
      181 aatgacacac agcaaaatga atccgtttat ttgggtactt ataatcctga tgacgctaga
      241 cgcgctgcgg gaattggctg gcgcttcgtc tattttagga tggctattaa cgttggtttg
      301 agcaagcaat aagtccggac gtgtagttac cgcagtccgg gcttattttt caggcgtgca
      361 gacgacgatg caaacgcgtc ccgaccagta gagcaatgac cagcatcaga gcaataaatg
      421 cgccgacgcc gttccagcca tagttatgcc agaaaacacc acccagcgtc ccggcaatac
      481 tcgaccccag atagtaactg aacagataca gcgaggaggc ctggccttta gcgcgttttg
      541 cgcgggggcc aatccagctg ctggcaactg agtgggctgc gaagaatcct gctgagaaga
      601 gtaacattcc ggcaaagatc agccacagcg agctgaataa ggtcatcagt aaaccaaaca
      661 gcataacccc cgtcgaaaac aacatcaccg gaccgcgccc gtagcgggtg gtcatggttc
      721 cggctttggg tgagctccat gtaccggtca aataagccag cgataataag ccaaccacgg
      781 cctgactgac atgccagggg gaaagcatca accgatagcc gatgtaatta aacagcgtga
      841 cgaacgaccc catcagcaaa aagccttctg cgaacaataa cggtaatccc cggtcacgcc
      901 agtgcagacg aaagttgata aataacgtct tagggcgcag cgaagtcggg cgaaaatggc
      961 gtgattccgg aaggattttc cagaacatca acgccgaggc tagcgcgaaa caaccgattg
     1021 ccgccagagc aattcgccag ttgaaaaagt ccgtgaagac accactaatt aagcgtccgc
     1081 tcatgccgcc aattgagttg ccgctgatat acaaccccat cgaaaaggcc acgaaactgg
     1141 ggtggatttc ctcgctaaga taagtcatgc caacagctgc cacgccactt aacgaaagcc
     1201 caatcaaggc gcgcataatc aaaatgccgt gccagctggt catcattgtc gaaagtaacg
     1261 tacaaatgga ggccaacagt agcgccgtga ccatcactgg tttgcgacca atggcatcgg
     1321 atagggggcc agtaaacagc aaaccaatag ccaacatcgc cgtggaaatg gacagtgaaa
     1381 tactactgtt cgcgggggtt aagccaaact cctgcgaaag caccggaagg ataggctgca
     1441 cacaatagag gagtgcaaag gttgccagtc cggcagagaa cagcgccagg gtgacgcgca
     1501 taaattgcgg cgtaccgcgt ttaataaatt gatttggctg agaaatgctt tgcttgtcag
     1561 tgtcgcttgc cggagcgcca tcaacagttg tagtacggct cacttgaaat ccttgctaaa
     1621 tatgcctgtg gatcaggctt atacataggt taggaaaatc gaattgttct gtctaatata
     1681 ttaataatct caaataagat gttttaaata tgaatattga acttcgtcat ctgcgttact
     1741 ttgttgctgt tgcggaagag ctgcatttcg ggcgcgccgc tgcccgcctg aatatttcgc
     1801 aaccaccgct aagtcagcag attcaggcac tggagcaaca aattggtgcc cgactgctgg
     1861 cacgaactaa tcgcagtgta ttgctgacgg cagcaggaaa acagtttctt gcagatagtc
     1921 ggcaaatcct gtctatggta gatgacgctg ccgctcgcgc tgaaagactg catcagggtg
     1981 aagcggggga gttgcgcatt ggttttactt cgtccgctcc ttttattcgg gcggtgtccg
     2041 atacgttatc gctgtttcgc cgtgattatc ccgatgtcca tttacaaacc cgcgaaatga
     2101 acactcgtga gcaaatcgct ccgctcattg aaggaacgct ggatatggga ttgctgcgta
     2161 acacagcgtt accggagacg cttgaacacg cagtcatcgt ccatgaaccg cttatggcga
     2221 tgatcccgca cgatcatccc ctggcaaata acccgaatgt aacgctggct gaactggcga
     2281 aagaaccctt cgtctttttt gatccgcacg tcgggacagg gctgtatgac gatattctcg
     2341 ggctgatgcg acgttaccat ttgacgcccg tcatcactca ggaggtgggc gaggcaatga
     2401 ccatcatcgg tctggtttcc gccggtctgg gtgtttcaat tttgcctgct tcatttaaac
     2461 gtgttcagct caacgaaatg cgctgggtgc cgattgctga agaggatgcg gtttctgaaa
     2521 tgtggttggt ctggccgaaa catcatgaac aaagtccagc ttcgcgtaac tttcgtattc
     2581 atctgctgaa tgctctcagg tgagggaaat ttcagcgaaa aagcccgaaa aatgtgctgt
     2641 taatcacatg cctaagtaaa aatttgacga cacgtattga agtgcttcac catagcctac
     2701 agattatttc ggagcgcgaa aatataggga gtatgcggtg gttgctgaaa accagcttgg
     2761 gcacattgat caaataaagc agaccaacgc gggcgcggtt tatcgcctga ttgatcagct
     2821 tggtccagtc tcgcgtatcg atctttcccg tctggcgcag ctggctcctg ccagtatcac
     2881 taaaattgtc cgtgagatgc tcgaagcaca cctggtgcaa gagctggaaa tcaaagaagc
     2941 ggggaaccgt ggccgtccgg cggtggggct ggtggttgaa actgaagcct ggcactatct
     3001 ttctctgcgc attagtcgcg gggagatttt ccttgctctg cgcgatctga gcagcaaact
     3061 ggtggtggaa gagtcgcagg aactggcgtt aaaagatgac ttgccattgc tggatcgtat
     3121 catttcccat atcgatcagt tttttatccg ccaccagaaa aaacttgagc gtctaacttc
     3181 gattgccata accctgccgg gaattattga tacggaaaat ggtattgtac atcgcatgcc
     3241 gttctacgag gatgtaaaag agatgccgct cggcgaggcg ctggagcagc ataccggcgt
     3301 accggtttat attcagcatg atatcagcgc atggacgatg gcagaggcct tgtttggtgc
     3361 ctcacgcggg gcgcgcgatg tgattcaggt ggttatcgat cacaacgtgg gggcgggcgt
     3421 cattaccgat ggtcatctgc tacacgcagg cagcagtagt ctcgtggaaa taggccacac
     3481 acaggtcgac ccatatggta aacgctgtta ttgcgggaac cacggctgcc tcgaaaccat
     3541 cgccagtgtg gacagtattc ttgagctggc acagctgcgt ctcaatcaat ccatgagctc
     3601 gatgttacat gggcaaccgt taaccgtgga ctcattgtgt caggcggcat tgcgcggcga
     3661 tctactggca aaagacatca ttaccggggt gggcgcgcat gtcgggcgca ttcttgccat
     3721 catggtgaat ttatttaacc cacaaaaaat actgattggc tcaccgttaa gtaaagcggc
     3781 agatatcctc ttccctgtca tctcggacag catacgtcag caggcccttc ctgcgtatag
     3841 tcagcacatt agcgttgaga gtactcagtt ttctaaccag ggcacgatgg caggcgctgc
     3901 gctggtaaaa gacgcgatgt ataacggttc tttgttgatt cgtctgttgc agggttaaca
     3961 ttttttaact gttctaccaa aatttgcgct atctcaattt gggccaggaa agcataactt
     4021 agactttcaa ggttaattat tttcctagtt tatatttgtg aagcataacg gtggagttag
     4081 tgatgctgga gcgtttcttt attaccggta cagacacttc tgtagggaaa acggtggttt
     4141 cccgcgcatt gctacaagcg ttagcctccc agggaaaaac agttgcggga tataaacccg
     4201 tagcgaaggg gagcaaagag acacccgaag ggctgcgtaa taaagatgcc ctggtgttgc
     4261 agagtgtttc aaccatcgaa ctgccttatg aagcagttaa tcctatcgcg ttaagcgaag
     4321 aagaaagtag cgtggcgcac agttgcccaa tcaattacac cctcatttca aacggcctgg
     4381 caaacctgac cgataaagtc gatcatgtcg tggtagaagg gactggcggc tggcgcagtc
     4441 tgatgaatga tttgcgtcca ctctctgaat gggtggtgca ggaacagttg ccggtgttga
     4501 tggttgtcgg tattcaggaa ggttgcatta accatgcact gctaacagct caggcgattg
     4561 ccaacgacgg gctgccgctc attggctggg tggctaaccg aatcaaccca ggactggcgc
     4621 attatgcgga agtcattgat gtgctgggta aaaaacttcc ggcaccgctc attggtgaac
     4681 tgccttatct gccgcgcgcc gaacagcgag aactggggca atacatccgc ttagctatgc
     4741 tgcgcagtgt gctggcggta gatagagtca cggtgtaacg tccgcgaaat taccgacgca
     4801 atgacgcagg caatcaataa accggggagt agctgatact ccccggtcat ttcacatatc
     4861 atcaacgtcg acataatcgg cgcgtgcgtg gttgccgcca acagtgtcgc catcccggtc
     4921 aatccgagta aaagcgtaat ttcttcgcca tcagggaacc ataatcccag gctacgacca
     4981 tacaacatgc caatggcaag accgataaat agcgtcggtg taaagacacc accgggtgcg
     5041 ccggaaccgc tactcgccag cacggcacac agtttacaaa ggaagatccc ggcaatgatc
     5101 attaacagtg gtggggcggt taaaaaggat tgtacggtgc tatagccgtt gccccacact
     5161 gcaggtgtga acagggaaag cagacccacg atcaacccgc ctagtgccag ttgccagggc
     5221 ggcgcaagtt tgagactcac aaatccacga tgacaggcgt tcattaacgt taacaacagt
     5281 ggtccgcaca gacctgccag cacacctgta ctgataatca gcgcatagtc actagcctga
     5341 accgttactg agagttgtac gctgtagagt aacgcgtcgc tatgattaat aagattgcta
     5401 accagcaacg ccacgacggc ggaaataatc accgggccga gagaggccaa catcatagtg
     5461 ccaaacagca cttcggctat aaataaactg ccagcaagcg gggcgcgata ggccgcagcc
     5521 attcccgccg cggccccaca ggcgatccat aatttccatt cctggcgtgg cgtaaaacgt
     5581 tgggcaaaac aggaggcggc aagggcagct aaaagaatca tcgcaccttc gcgaccaatt
     5641 gcactgccgc tggttactac cagcagcgag gcaagcgatt taaccaggct tgctgcgtaa
     5701 tcgaactgtc catcggtttg caacgcttcc atgtaatcgg tcggcgcatg agggcgttgt
     5761 tgggtaaatt tctgccagcc catcagcaac aaacccgccg ccagtccgcc gagcgccgga
     5821 gttagcagcc gtcgccaggg ggaaaggttt gtcgctgcat tgaccagact gccggagtca
     5881 ttattgagga acaaccactc cagtagcagc atcgcatgac gaaacccggc aacggcaaag
     5941 gccgcgagaa taccgacgac tgttgcgata agcagacggc ggaacatagt gcgcagatcc
     6001 ggatagatgt gtagatgatg catgagcaga aatgggcaat gaaacacggg cgactatttt
     6061 gcggcaaaag aggccaaccg caaaagcagt gcgtgcttta agagataaaa aaacgtgaca
     6121 ttgtaactta ttattgccga atcgcgcgta atcgtcacca tccggcaata ttacggtgat
     6181 cctatcgaaa gagtggttta accgcgacag gaatcaacag ttgtgattgc cattgcgcca
     6241 gcgttaagcg agccagttca cccagtgcac ggtagaaagg gtgttctgct ttttcgataa
     6301 aaacatcaag aaaacgtgtt gaccacggaa aaaggtgcca tgccagcaat tcttcgcatt
     6361 ctgtctggcg accattctcc gccaaccacg cagccatcaa cagcagtgaa ccaaaatgat
     6421 cttccggttc gttttgcttc atttcaaact gaatgccttt ctcgcgcatc cactgacgaa
     6481 gtgccaatgt tgaatcgcca aacagcacag attcgcgatc cagccagacc gatccccacg
     6541 gcggagacgg cagtgcccac gggccgacaa acaaacgctg ccaggcctgg gcgtgagtct
     6601 cttcacactg tgtctgaaaa gccgtcacca gcggcgctaa tgacgcttct ggtagaggcc
     6661 actgagtttc ccagccgtca ctggtgagta ccgcaaccag aggtgcggct tccgcactct
     6721 ctggagcgta ataaaacagc gcgcccaaca cgcgcgccgc gacagaaaaa ttatcttgct
     6781 gtgaaaaatg ggtcattcct aacatcctga aatgtgcggg ttgccccgca ccaatttgtt
     6841 aacctgcaat agccattcct actgtcatgt gcaggccata aaagagcaca cgcccgatca
     6901 tctcaccaac aagtatgagg attagcccca gaacaagtcc ggcaacgtga ggttcacggc
     6961 gacgaatgag cggacaaagc cagcatccaa gacctgcgca taacaatacc acacgccaga
     7021 cctgtaagga ggcgtaatct ggtaccagtg cgctggcttg ttgcacggat gagtgaatag
     7081 acgccagaga caaaccttgc agcacaatca ccccggcaca agcgatcagc gcgagaacgc
     7141 taatgatggc aaatggtgtg gtattaaatg taacgcgggc tgcccgcaga attgcggcag
     7201 caagtattgg accgctcaac aataccgtca ggaagaaagc cagcgtagtg taaccgttat
     7261 gccaggttgg cacagtgtcg atttgataca cgcaggtcat catccagacg aaaatgacgc
     7321 cgagcgccat gctgacaagt aaccagagtt tccccaacgc ttgcggcatt ttaccgatga
     7381 ccgccaccag ccaccatagt ccgccaacgg caaaaaatat ggaaccggca gcaatctcgt
     7441 tactcagacc agaagctccg attcgattca gcgagttaaa cgcccgcagt ggcgatccca
     7501 ggtgcatgac agaggccata aacccgatgc ccatcaacag ccagaggaaa aacatgccac
     7561 ggacaatacg ctgacggtct gcgtcatttt tggcggcgaa ccagccaatt ccgctaacaa
     7621 tcagcgcgcc tactacgcat tgacccaata cggtaaagat aaccagtggc cactcatgcc
     7681 atccatttcc cattttacac ttcctccgga ttagccagat aaccagtggt atcgctggtc
     7741 gggcggctgt tggcgttagg tttgataacg atattgggtt ttgtgaaatg cgcgcgcggc
     7801 agtggcgcga cggcagccag agtgccgtgc ttctggcgca gctcttcaat tggaccgaac
     7861 tcaagtgcac gcagcgggca ggattcgaca catatgggtt gtttcccctc ggcgacgcgc
     7921 gaataacaac catcgcactt cgtcatgtgc cctttttcag cattgtactg tggcgcgccg
     7981 tacggacaag ccatgtggca gtagcggcag ccgatacaaa catcttcatc aaccaccaca
     8041 aagccatctt cacgcttatg cattgcgccg ctggggcaga cttttgtaca tgcggggtca
     8101 tcgcaatggt tacaggagat ggagagataa taagcgaaca cgttctggtg ccagacgcca
     8161 ttatcttcct gccagtcgcc gccagcatat tcataaatac ggcggaaact gacttccggg
     8221 ccaagatctt taaaatcttt gcacgccagt tcgcaggttt tacagccagt acagcggctg
     8281 gaatcgataa aaaatccata ttgtgtggtc acgggctact ccttaaacct tttcgatctg
     8341 gacaagattg ctgtgcgacg ggtttccctt tgccagcggt gaagggcggt gagaggtcag
     8401 aatattgata ctgccgccgt gatcgacccg gtcaccaaac atatccgctt taagccaagc
     8461 accttgcccg atggcggtaa cgccaggcag aatacgcgga gtcacttttg cggcaatcag
     8521 catttctcca ttattgttaa atacccgcac ggtatcgcca tgacggatac cgcgtgcctg
     8581 agcatcaatg gggttgatcc acacctcttg tgggcaggcc tgctgtaaca catcaatatt
     8641 gccgtagctg gagtgggtac gcgctttgta atggaagccc gttaactgca gtggataggt
     8701 tttccgcagg ggatcgtccc agccatcaaa acctggggta tacgcaggaa ggggatgaat
     8761 aacttcatct tttttcaatt cccaggtatc cgcaatcttc gccagtcgtt cagaataaat
     8821 ttcgattttc cccgaaggtg ttttcaacgg gtttgcctgt ggatcttcac ggaatgcgcg
     8881 gaaagcgacg tagtgttctt ccgggcattt tttcttaaag atcccggtcg ttttcatctc
     8941 ctcgtagtcg ggcatctcag ggttacgttc cttcgttttc gcatggagat atttgatcca
     9001 ttcatgctga ctgcgacctt cagtaaaggt ttgataaacg tctggtccta agcgtttggc
     9061 gacttcactc agcatccagt agatgggttt gcgttcaaat tttgctgagg ttg
//
LOCUS       DAJVQG010000149         9086 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJVQG010000149 DAJVQG010000000
VERSION     DAJVQG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9086
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.149"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..586)
                     /gene="araH"
                     /locus_tag="OQM83_003326"
     CDS             complement(<1..586)
                     /gene="araH"
                     /locus_tag="OQM83_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCS1521515.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTT"
     gene            complement(601..2115)
                     /gene="araG"
                     /locus_tag="OQM83_003327"
     CDS             complement(601..2115)
                     /gene="araG"
                     /locus_tag="OQM83_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCS1521516.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(2185..3174)
                     /gene="araF"
                     /locus_tag="OQM83_003328"
     CDS             complement(2185..3174)
                     /gene="araF"
                     /locus_tag="OQM83_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HCS1521517.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            3971..4474
                     /locus_tag="OQM83_003329"
     CDS             3971..4474
                     /locus_tag="OQM83_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCS1521518.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(4553..4804)
                     /locus_tag="OQM83_003330"
     CDS             complement(4553..4804)
                     /locus_tag="OQM83_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCS1521519.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(4919..5005)
                     /gene="azuC"
                     /locus_tag="OQM83_003331"
     CDS             complement(4919..5005)
                     /gene="azuC"
                     /locus_tag="OQM83_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCS1521520.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            5268..5591
                     /locus_tag="OQM83_003332"
     CDS             5268..5591
                     /locus_tag="OQM83_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCS1521521.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            5762..6259
                     /gene="ftnA"
                     /locus_tag="OQM83_003333"
     CDS             5762..6259
                     /gene="ftnA"
                     /locus_tag="OQM83_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCS1521522.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(6297..6536)
                     /locus_tag="OQM83_003334"
     CDS             complement(6297..6536)
                     /locus_tag="OQM83_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCS1521523.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            6727..7938
                     /gene="tyrP"
                     /locus_tag="OQM83_003335"
     CDS             6727..7938
                     /gene="tyrP"
                     /locus_tag="OQM83_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCS1521524.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(8000..8665)
                     /gene="yecA"
                     /locus_tag="OQM83_003336"
     CDS             complement(8000..8665)
                     /gene="yecA"
                     /locus_tag="OQM83_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCS1521525.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(8861..8947)
                     /locus_tag="OQM83_003337"
     tRNA            complement(8861..8947)
                     /locus_tag="OQM83_003337"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8911..8913),aa:Leu,seq:taa)
     gene            complement(8960..9033)
                     /locus_tag="OQM83_003338"
     tRNA            complement(8960..9033)
                     /locus_tag="OQM83_003338"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8999..9001),aa:Cys,seq:gca)
BASE COUNT         2277 a         2292 c         2082 g         2435 t
ORIGIN      
        1 aggtggtttt attcagcaac aaaccaaaga taatcagaca cgcgacggtg agccagattg
       61 gcgcaggcag accgaaccag ttagcgtaac caagggcaaa gaagctttca tcttcgatac
      121 cgaccgcttt accgtctgaa atgatgtacg ccagacctcg aacaatctgc atcgttgcca
      181 gtgttgtgat cagagcattt attttcagtt tggcgataac aaagccattg accaggccac
      241 agagaatgcc aagcagcaac cccgctgcca cgccaatcca caggctttca gtcaggttga
      301 taaccaccgc cgtggtgaca cccgcacagg caattacgga ggcgacagaa aggtcaaagt
      361 caccggaagc aaggcagaac aacatgccac acgccaccat ccccgacatg gaaattgcca
      421 ggcccaaccc tttcatatta atgaaggtgg caaaatttgg gacaaaaatg gcacaggcga
      481 taaagagcac cgcaaacacc accagcatgc cgtactgatc ccagatacgc ccgaagctga
      541 atgacgactt aggtgcgcca gaacccgatg tagaaacaga agacatcata ctctccttac
      601 tcaggcaact gcctggctga ctttaggcat cgcaaggctc agtgcctgac gctcatctgc
      661 ctgctcgtgt aacaattcac cggcgatttc accttcccgc atcaccacaa tccggtcggc
      721 aacgccgagg acttcaggta agtcgctgga ggcaaacagc accgccacac cctgcgccgc
      781 cagcgcataa attacgttat atatttcgtg cttagcacca acatcaatgc tgcgcgtagg
      841 ttcatccagc aaaatgacct tcatctcttc cgataaccag cggcccagaa tggctttttg
      901 ctgatttccg cctgagagat tcatgatcag ttgctcagcg cccggcgttt tgatgttgag
      961 cgaacgaatg tgctgatcgg cattgttttc ttcccaaccg ttgttgatta cacaaccgcc
     1021 gagcacatgt ttgcgtctgg cactgatgtt gatattgtcg cgaacggagt gcacgggaat
     1081 aatgccttcc gctttgcgat cttccgggca gagcatcatg cctgcggcaa tggcgtggct
     1141 cggtttacga atatcgatcg gctgttggtc gatataaacc tgaccggcgg tgatttgcgt
     1201 cccgccaaac aagcctttca ttaattcgct acgccccgcc cctaccagac caaacagccc
     1261 aacaatttca ccactgcgaa ccgccagact tattggcgta cgcacgcctg gtgctttcac
     1321 agcatcaaga cgcaggcgct cctcgccata actacgaggt tgccagccgt agatatcgcc
     1381 aatgtcgcgc ccgaccatcg cctgcaccag cgcgtcgtgg tcaacctgct gcatatcggt
     1441 aaaggttttg acataacgtc catctttaaa gacagtgatg gcatcgctga gggcaaatat
     1501 ttcttccata cggtgagaaa cgtataagat cacccgcccc tcttttcgca gttcacgaat
     1561 aacgcggaaa agattgtcga tttcgcgggc agaaagggag ctggttggct catcaaaggc
     1621 gataatttta gcgttacgcg ccagcgcctt ggcgatttca accatctgcc actgaccaat
     1681 ggagagatat ttcagcggcg tgtccgggtc aatatccata ccaagatgtt taagttgtaa
     1741 acccgcctca taattcagca atgagcgatt cacaatgccg cctttatgcg gcagctggcc
     1801 gagatagatg ttttccgcga cggtcatttc cggcacgaga tgcagttcct ggtaaataat
     1861 cgccacaccc gcgttaagtg ctgcggtcgt gtcggaaaag gacatttcct gcccattaat
     1921 cactacagaa cccgtggttg gcgcatagtt gccgctgagg atttttaaga gagttgattt
     1981 tcctgcgcca ttttcaccca tcaacgcatg aacctgaccg gcatagcagt caaaactaat
     2041 atccgtcagc gccttaacgc cgggaaacgt tttaccgatg ccgcgaaatg agagatacgg
     2101 ggtagactgt tgcataacgt ctccgtgaat cactggtcgt tcacaccccc tctgcatcat
     2161 gcagagggga atttttccgg caaattactt accgcctaaa ccttttttct ccagttcttc
     2221 tttaaagttg tcacgcgtga tcagcaccac gtcggtaact tcggtaaatt ttggcggttc
     2281 aacgtctttt gctacccagt tgtaaagcat ttcgctggat ttatagccat gtacgtccgg
     2341 gcttggcagc agggaaccgt agaagccggt tgcctgtgct ttagacagtt cgctcaacgc
     2401 atccacaccg ttaatgccaa tgccgatgat atcggcagct ttaaagccct gaccttccgt
     2461 cgcgcgtacg ccgcccagca cggtgctgtc gttcataccg acgatcagcc aatgtttaac
     2521 ttccggatgt tgaaccagca ttgagttggc agcgtcaaat gcccccggga tgtcgttaga
     2581 tttggtaggt acctgataaa tttgtttttc cgggaatccg gccgctttca gcgcatccat
     2641 agaacccgtt gtacggcgac gtgccgtatc cagttcgttg gcggtaatcg ccatcaccgc
     2701 gctttctttg acatcccagc cacgtttctg catctcttta tacagttcct gaccctgacg
     2761 ttcgccaatt ttagtcgccg ccatcatcac cagcggaacg gtatccattg gcttaccttt
     2821 ggcgttaaca aactggtcat ccacggcaat gactttcata tcgtagccac gcgctttcgc
     2881 gacgatggca gagccgagtt tggggtccgg agtacaaata acgaaacctt ttgcgccact
     2941 ggcagccagg ctgtcgatcg cgttcaatgt tttttcgcca tccggcacgg caatcttaat
     3001 aacctcaaac cctaaatcct tcccggcttt atcggcaaac ttccattcgg tctggaacca
     3061 cggctcttcc ggttgcttca ccagaaaacc gagcttcagg ttctccgcca tagcggattg
     3121 tgacataacg gctgccagac caatggctgc cagggcttta gtaaatttgt gcatggttct
     3181 ctccagcttt agtgtcgttt tgtgtagggc aaaaacgaat gactttcgtt aaattaatcg
     3241 gaaaacaaag cattaccttt tatctaaaag ataagtgacc gtgttgacat agttttagcg
     3301 agaaattaat tctccatagg agagcaatat cacatcgcag aattacagtg agaacgtgca
     3361 taaatttagc gggaaaagac ataagggaaa gccaatttgt cagacaaatt gtcgaatgca
     3421 cagcagatta atccataaga ttagcctgga aatccttgtt gtctttggta cccatgcggg
     3481 atgtcttctt tttaaccagt caataggccg cattacctgg cgttgagttt ttgaaatggt
     3541 gtaataaccg caactcaaag atgtggaaaa tgcacgtcat tcatttcgcc attaattatc
     3601 actgtgctca ttaattaaca gaacacgtat aatgagagcc atctcgcaaa aatgaaaaaa
     3661 cgttttataa aatcatcact tcatcatgaa ttcaaattca ttgattaata tcaacaagat
     3721 acaaaaagca ctatcattaa aattcattgc agttacattg atttcatcaa tgaaatgtaa
     3781 aaatatataa acttgatgat ttaagcattt tcttataccc gttcagacgt tattcttatt
     3841 tcagatcatc gtcagaattg actccacgat cacatttcgg accggcagaa aggaattatt
     3901 ctgcaaacag taattatggt gttttgattt atcttgcacc tctccacttc tggatataag
     3961 gatattaggt atggcaaccg ctggaatgct tctcaaactc aactctcaaa tgaaccgcga
     4021 gttttacgca tccaatctct accttcacct gagtaactgg tgttctgaac agagtctgaa
     4081 cggcaccgct actttccttc gcgcccaggc acagagtaat gtgacccaaa tgatgcgcat
     4141 gtttaacttt atgaagagtg tcggcgctac ccccatcgtt aaagccattg atgttcccgg
     4201 tgaaaaactg aactctctgg aagaactgtt ccaaaaaacg atggaagaat acgagcaacg
     4261 ttctagtacg ttggcacagt tagccgatga agcgaaagaa ctgaatgatg attcaaccgt
     4321 caatttcctg cgcgatctgg aaaaagaaca gcagcatgat ggtctgttgc tgcaaaccat
     4381 tcttgatgaa gtgcgcagtg cgaaacttgc gggtatgtgc cctgtgcaga ccgaccaaca
     4441 tgttctgaat gtcgtgtcac accagctgca ttgatcatca tcggcgctaa tgcattgcgc
     4501 cgatgaaggt tttgagaaac cgctgcctca tctgtttgaa gcagcggttt ttttaatggg
     4561 attcaccctg tggggtaaac ttgagttcaa taagcgcgat ggctttttgg attgcccgca
     4621 tggtgaccgg gtctgcggcg gcgggatggt tagtaaagtc gatattcttc agctgactgg
     4681 acattttttc acgaacttca acgggcgcga ttacatcgag aacatccaga atttgtttga
     4741 taaccaactg gcaagcaacc acatcagaaa ccagttcctg atcggcattc agcggctggg
     4801 acatcgtaaa ctcctgatag cattttgaaa gccgttatag tagcggcttc acatcttcag
     4861 cgatagtcac atccaccgtc atcaggacac aaaaaaacct gccggagcag gttttttgtt
     4921 atcggaacat attgcctggc ggtacgtctt tgaacgtctt gcaatagtta ttgaacatac
     4981 ttttcaggat tttgcgcagt ttcatcgcgg cactccgacc atttgttata caggtgttat
     5041 tgtctttgcg cttataatat gacaaccatc acaaaaatca atctttatgt gatacaaatc
     5101 acataaatac ccctttaatg ttataaaaat gataatcaaa aaacagcccc cctatttctg
     5161 acacctacag atggcaagaa atagcgcctg ccaggcgtct tttccggcca ttgtcgcagc
     5221 gctgtaacgc gtaaaatagt gctttctctt actcttctgg ctggaccatg agacttctga
     5281 ttctgactct ttcattaata acgctggcgg ggtgtacggt tactcgccaa gcccatgtga
     5341 gcgaagttga cgcggcaaca ggtattgtac ggttggttta tgaccaggct tttttgcagc
     5401 atgcccatac cgatcgttat gtcagtcgcg ggatcgccga tcgcgcatgc cagcaggaag
     5461 gctataccca cgcggtcccc tttggtcagc cagtaggcaa ctgtagcctt tttgcgggtt
     5521 ccttatgttt gaatacggaa ttcactttgt catatcagtg ccatcattcc gccttccctg
     5581 tctttctcta aatattttaa tcagcgaggg gatcttcgct gattaaagaa atagcggtaa
     5641 tgcgttttat tcccattcgt atttttaata attgaagttt atattttacc ttttgcaaat
     5701 aataaaataa caaattatag tgacgccaca gcaacaaata taacctttgt ggagcactat
     5761 catgctgaaa ccagaaatga ttgaaaaact taatgagcag atgaacctgg aactgtactc
     5821 ttcactgctt tatcagcaaa tgagcgcctg gtgcagctat cataccttcg aaggtgctgc
     5881 cgcgttcctg cgccgtcacg cccaggaaga gatgacgcat atgcagcgtc tgtttgatta
     5941 cctgactgat accggcaatt taccgcgtat taataccgtt gaatctccgt ttgctgaata
     6001 ttcctcactt gatgaattat tccaggaaac ctataaacac gaacaattaa tcacccagaa
     6061 aattaacgaa ctggctcatg ctgcaatgac caatcaggac tacccaacat ttaatttcct
     6121 gcagtggtat gtttctgagc agcatgaaga agagaaactg ttcaaatcga ttattgataa
     6181 attaagcctg gcaggcaaaa gcggcgaagg tctgtatttt atcgacaaag aactctctac
     6241 cctcgacaca caaaactaat gctctccggc ggcaggttta ctgccgccgt ttcaaatcag
     6301 tgacggcaaa tcttactctg atcggtcgaa aaaccgtctt tcgaaggtat aaatttgcct
     6361 tttgctgcca gaaacgctac cagctctccc gctgtcatcc cttctgccga gcaggtgtga
     6421 aaacgtgcct gttcaccaaa acgcgcttta atcgcagctt ccagactggc atgcgtatat
     6481 tgctcgcctg attcaatcat catatttaac acttcatgac cgtgaataga gtccatcgtt
     6541 cctcctcaaa aaaagcctag cgtagcgatt gccgcttatg aagactttgc gccagcgcag
     6601 gactgaatgc tttttattgt acatttatat ttacactata tgtaacgtcg gtttgacgaa
     6661 gcagccgtta tgccttaacc tgcgccgcag atatcactca taaagatcgt caggacagaa
     6721 gaaagcgtga aaaacagaac cctgggaagt gtttttatcg tggcgggaac cacaattggc
     6781 gcaggcatgc tggcaatgcc gctggctgcg gccggtgttg gttttagcgt tacgttaatc
     6841 ttgttgattg ggctttgggc gttgatgtgc tacacggcgc tattactgct ggaggtgtac
     6901 cagcatgttc cggcagatac cggtctgggc acgctggcaa aacgctatct gggacgctac
     6961 ggtcaatggc tgacgggctt cagtatgatg ttcttaatgt atgctctgac tgcggcatac
     7021 atcagcggtg tcggtgaatt gttggcctcc agcatcagcg actggacagg tatttcaatg
     7081 tcggcaaccg ctggcgtgct gttgttcact tttgttgccg gtggcgtggt ttgtgttgga
     7141 acatcgctgg tcgatttatt taaccgtttt ctgttcagcg ccaaaattat ttttctggtg
     7201 gtgatgctgg tattgctgct accgcatatt cacaaagtga atcttttaac cctgccgttg
     7261 caacaggggc tggctctgtc agcaatcccg gtgattttta cctcatttgg ttttcacggt
     7321 agcgtgccga gtattgtcag ctatatggat ggcaacgttc gtaagctacg ctgggtgttt
     7381 ataaccggta gtgcgatccc cctggtggca tatattttct ggcaggtggc aacgcttggc
     7441 agcattgatt caacaacctt tatgggattg ctggctaatc atgctggatt aaacgggctg
     7501 ttacaggcgt tacgcgaaat ggtggcctct ccgcatgttg agctggcagt gcatttattt
     7561 gctgatttag ccctcgccac gtcatttctc ggcgttgcgt taggcttatt tgattatctg
     7621 gctgatttat ttcagcgttc aaataccgtt ggtggacggt tgcaaactgg tgcaattacc
     7681 tttctgccgc cgttggcgtt tgcactgttt tatccacgag gatttgtgat ggcgctgggt
     7741 tacgccggtg tggcgctggc ggtactggca ttgattatcc cttcgctgtt gacctggcaa
     7801 agcagaaagc acaatcctca ggcgggttac cgggtcaaag gtggtcgtcc ggcgctggtg
     7861 gtggtgtttc tctgtggtat tgctgtgatt ggcgtgcaat ttttgattgc ggcagggttg
     7921 ttaccagaag tggggtgatc agatagcctc aaattcctta ttgggtgcca gaattaacgc
     7981 tgacacccaa tttggcctct taatgcaggc agcactgctt aaatttctta ccactaccgc
     8041 acgggcaagg atcgttacgc cccggtttct cttctgcttt gatcggttgc tgaacagctt
     8101 tttcctgcgg atgcgccatc cagtacgcat gtagatcaag cgccgccagt cgaatggcat
     8161 ctacgctctc ttcaaacgct tctggcgaca tcttttctac ccgctcgaag ttttcctcag
     8221 taccgtgcag cgcaatcgcc tccagcgctg gttttaacga atcgggcaac gttgaccagt
     8281 cagaaagtgc cacgccccgc atatagccaa agcaccactc ctcaacaatc gtcagctcgc
     8341 tgccatcaac ttctcgcaag ccgaataacg gctcaaactg ctccgggaat tcgttcagac
     8401 gctctgcggt atcggccata tgttgaaaag ccaggttcat aaagcgcgtc atctctttct
     8461 ctgacgccca gcgcggcaca tagtcagccc caccccacac ggcaaccagc cactgttccg
     8521 gttcaatctc ttgcggagaa ctcaacaccg ccgtcaataa accgtccagc tccgccacat
     8581 caaggatggc gtggttagtg ttgtatttgg tcagaatatc gtccagccat tccaactcac
     8641 tttcgtttaa cggtcccgtt ttcatacgct tttccttgtg gatctcaact cgccagcacc
     8701 tatcttacat gccggtccgt atcagagata ctttttgagt ggctttgccg gtgattaaaa
     8761 attaaggagg gtgtaacgac aagttgcagg cacaaaaaaa ccacccgaag gtggtttcac
     8821 gacactgctt attgctttga ttttattctt atctttccca tggtacccgg agcgggactt
     8881 gaacccgcac agcgcgaacg ccgagggatt ttaaatccct tgtgtctacc gattccacca
     8941 tccgggctcg ggaagaaagt ggaggcgcgt tccggagtcg aaccggacta gacggatttg
     9001 caatccgcta cataaccgct ttgttaacgc gccaaattct tcaggccttt cagccagaca
     9061 tccgcttgac gccgatgtct tttaaa
//
LOCUS       DAJVQG010000150         9045 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJVQG010000150 DAJVQG010000000
VERSION     DAJVQG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.150"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(43..969)
                     /gene="glsB"
                     /locus_tag="OQM83_003339"
     CDS             complement(43..969)
                     /gene="glsB"
                     /locus_tag="OQM83_003339"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCS1521526.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(1033..2421)
                     /gene="sad"
                     /locus_tag="OQM83_003340"
     CDS             complement(1033..2421)
                     /gene="sad"
                     /locus_tag="OQM83_003340"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HCS1521527.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            2522..3403
                     /locus_tag="OQM83_003341"
     CDS             2522..3403
                     /locus_tag="OQM83_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1521528.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            3480..4595
                     /gene="yneK"
                     /locus_tag="OQM83_003342"
     CDS             3480..4595
                     /gene="yneK"
                     /locus_tag="OQM83_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCS1521529.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLFLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLELFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            4745..5935
                     /gene="ydeA"
                     /locus_tag="OQM83_003343"
     CDS             4745..5935
                     /gene="ydeA"
                     /locus_tag="OQM83_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCS1521530.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(5960..6625)
                     /gene="marC"
                     /locus_tag="OQM83_003344"
     CDS             complement(5960..6625)
                     /gene="marC"
                     /locus_tag="OQM83_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCS1521531.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            6837..7271
                     /gene="marR"
                     /locus_tag="OQM83_003345"
     CDS             6837..7271
                     /gene="marR"
                     /locus_tag="OQM83_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCS1521532.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            7291..7674
                     /gene="marA"
                     /locus_tag="OQM83_003346"
     CDS             7291..7674
                     /gene="marA"
                     /locus_tag="OQM83_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCS1521533.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            7706..7924
                     /gene="marB"
                     /locus_tag="OQM83_003347"
     CDS             7706..7924
                     /gene="marB"
                     /locus_tag="OQM83_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCS1521534.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(7981..>9045)
                     /locus_tag="OQM83_003348"
     CDS             complement(7981..>9045)
                     /locus_tag="OQM83_003348"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCS1521535.1"
                     /translation="VTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTVFTRYKEKVK
                     YWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYELVASALAVQT
                     GKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARGYYPQHMLNY
                     FARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVEPRDLVSNPY
                     IDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRIDYF
                     ASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTLER
                     IRKASFYWYRDLIANNGENI"
BASE COUNT         2323 a         2307 c         2065 g         2350 t
ORIGIN      
        1 ttgaacggga taaacgcgcg aagagtggat cgagagactg cattaataaa ccgaacgccc
       61 taactgtttc gtcaattgtt caaggacggc gatacccgca agcgagttac ctgcatcatc
      121 cagttccgga ctccagactg cgatggccat ttcatgcgga acaatcgcca caataccgcc
      181 accaacgcca gatttcgccg gtagtcccac ccgccaggca aactcccccg cgttctggta
      241 cataccactg gtcgccatca gcgcgttaat ttgccgcgcc tgcattggcg tcactacagg
      301 ttcatcaata tgaatagctt tcccctgatt agccagaaag acaaacgtcc gagccagctc
      361 tacacagctc attttcagag cgcagtaatg aaagtagttt tgcagaacgg ttgtcacgtc
      421 atgatggaaa ttgccaaacg acttcatcag ccaggcgata gccgcatttc gcgcggaatg
      481 ttcaaattcg gaacgcgcta ccaccgtatc gtatgaaata tcagacacac cgcttaagcc
      541 gcgcacgact tccagcatac gttgccgtgg tgcgcttaat cgcccttgca acatatcgca
      601 gaccaccagc gcaccagcat taataaacgg attacgcgga ataccctgtt ccatttccag
      661 ttgcactaaa gaattgaacg gtgatccaga cggatctttg ccgacgcgtt gccagatttc
      721 ctcttcggag taatgacgca tggcgacaac gagactcagc actttggaaa tagactgaat
      781 ggaaaaacgt tcttgcgcgt ctccggcctg aaaaagctgt ccgtcaacgg tacagatagc
      841 aatgcccaat cgggaaccgt ctactgtagc cagcgccgga atataatccg cgactttacc
      901 ctgaccaatg agcggccgca cttgccgcaa gatgttttct aaaattgcat tatccatggc
      961 gactgccact ttctactcct ggaccgcagg tctgaaaaga cctgcgagta tatcagagct
     1021 gaatatgtcg cgtcagatcc ggtctttcca caccgtctgg atattacaga attcgtgtaa
     1081 gccgaaatgg gaaagctcac gaccaaagcc actctttttc acgccaccaa aggccactcg
     1141 cgcgtcgctg gcacaataac cattgataaa caccccaccg cattccagac gtgccgccat
     1201 ctgtctggcc tgtgtttcgt cggtggtaaa aatggtcgct gaaaggccga actcactatc
     1261 attagccagt tccagcgcat gttctgcatc tttcgcaacg gtgattgccg caacggggcc
     1321 aaacatttct tcccgaaacg cggtcatttc tggggtaaca ttcgccagaa ccgttggcgg
     1381 atagtagtta cctgccccag ccatcttttc cccgcccagt aacaaacgcg caccctgcgc
     1441 cagggttttc tctacctgat gatgcagctc atcacgtaaa tcaaaacgag ccattggtcc
     1501 gagagtgttc tcttcgtcac ggggatcgcc cattttcaag gctgccgcag ctgccacaaa
     1561 acgttcggta aatgcagaag caattccctc ttcgataata aagcgttttg ccgctgcaca
     1621 tacctgtccg gtattctgat aacgtccggc taccgccgct ttaaccgcca gttccagatc
     1681 ggcatcgtta agcacaataa acggatccga accgcccagt tccagtacgc attttttcag
     1741 tgccgctcca gcctgtgcgc caatagccgc tccagcacga acacttccgg tcaccgtgac
     1801 agcagcaatg cgcgagtctt taatcatctg actgacaccg tcgttgtcgg cattcagcca
     1861 gccatatacg ccttgcggga ttcccgcatc tttaaacacc tgggcaatga gctgagcaca
     1921 tcccatcaca ttcggcgcat gtttaagtaa gtagccgtta cctgcaagaa tgatgggaac
     1981 agcgccacgc atcacctgcc ataacggaaa attccacggc ataatcgcca gaatcgtccc
     2041 caacggtcga tactcaataa ccgcctgctg attttccacc agtgtaggtt ccgccttcag
     2101 cattgccgga ccatgttctg cataccagtc acacaaattc gccgatttcg ccacttcagc
     2161 gcgcgcctgg ttgattggtt tgcccatttc gcgggtgatc atttgcgcca tttcttcgct
     2221 acgggcgcgc agagccttac cgatatcacg cattttttca gcacgataat ctatatttgt
     2281 ctcgcgccag tcgcgaaagc ctgctgccgc cagctcaagt gcgttttcga tatcgttagc
     2341 gccagcccac ggcagcacag aaagttgttc acccgtggca ggatttattg aaattgcatg
     2401 agttgccgga gtaatggtca tcggggtatc tcctttatga gtcatggtat gaagatacgc
     2461 agatttactc ttgctttaaa atgaataata ttaagccact tattcacgaa tcgagaatgt
     2521 tatggatctg acccaactgg agatgttcaa cgcggttgcc gaagctggca gcattaccca
     2581 ggctgcagca aaagtgcatc gcgtaccgtc gaatctcact acccgtctac gccagctgga
     2641 aacagaactg ggggtagatc tgtttattcg cgagaatcag cgtttacgtc tctctcctgc
     2701 cgggcataac tttttacgct atagccaaca aattctcgcg ttagtggatg aagcgcggag
     2761 cgttgtcgct ggcgatgagc cgcaaggttt attttctctt ggttcgctgg aaagcaccgc
     2821 agcagtgcgc attccggcca cgctggcgga attcaaccat cgttatccca aaattcagtt
     2881 ttcactttcc accggccctt ccggcactat gctggaagga gtgctggagg gaaaactgaa
     2941 tgcggcattt attgatgggc ccattaacca tactgccatt gacgggatac cggtataccg
     3001 cgaggagctg atgatcgtca cgccacaagg tcatgcgcca gtaatccgcg ccagtcaggt
     3061 taacggcagt aacatttatg ccttccgcac caattgttcg tatcgtcgcc acttcgagag
     3121 ctggtttcat gctgacggtg ccgctccggg aactattcac gagatggagt cgtatcacgg
     3181 gatgttggcc tgtgtggtcg caggagcagg cattgcgctt attccacgct ctatgctgga
     3241 aagtatgccg gggcatcacc aggttgaagc gtggccgtta gctgagcaat ggcgttggtt
     3301 aacaacctgg ctggtctggc gtcgcggtgc gaaaacgcgt ccgcttgagg catttattca
     3361 actactggat gtgtctgact cggcaaaaca gagttaccaa tgaactattt tgataattct
     3421 tgcggctaat atgctctata tagtgatgtt ccgatgactt atgactatat ggggcaaata
     3481 tggttacgcc agtaagcatc agcaattaca tatctcttcc tgatgatttt cccgttcgta
     3541 atattgcacc acaggtaaaa gaagttttaa aggactttat tgatgcgcta agtacaataa
     3601 tatgtgatga agagtggcgc acgtctttaa acatcaactc agccacaaaa aagatattta
     3661 ataaccttga taacttatca tatattcaga ggacttcttt ccggggtaac gacacgctat
     3721 acaatgaaaa ggttcagttt aaactcactt atcccgtcaa gaatggaaga cacaaagaaa
     3781 atattgaatt tcaggtagta ataaatttaa gccctattta tttagataat tttcgccatg
     3841 atggagaaat taatattttt tgcgctccca acccaaagcc tgtcactatg gggcgcgtat
     3901 ttcagaccgg cgtcgagcgt gtgctctttc tgtttctgaa tgattttatt gaacaatttc
     3961 caatgttcaa ccctggcgtt ccgatcaaaa tagcgcatac accacatatt gaacccctgc
     4021 ctccggatca ccataccgct gcagattact tacgccagtt tgatttgctt gtcctgaatt
     4081 ttatctctcg cggtaatttt gtcatactcc cccgattatg gaataactct gaggttcaca
     4141 gatggttcgt caataaagat cctaacttga tcaccgccat tctcgacata acggacagcg
     4201 aattaaaaga ggatttattg caaagcctga tggattcatt aggttctaac aaacatgtac
     4261 tacccgaagt ctgcatctgc tttttattcc ttttagcaga acaagaatct cctcattttc
     4321 aggacttgtt tttatttttc gccaatatgt tattgcacta tcaccaattt atgaatccca
     4381 atgaaagtga tttgaatgac gtgttgatgc cagcatcatt aagtgatgat aaaattatca
     4441 agcatatggc gcgcaggacc ctcgaactgt ttgtaaaaaa tgaaacaccg ccaaaagtta
     4501 ctcacgaaga cctggtgaaa aacaggcctc gctcccctgt cagaccacct atacccgcaa
     4561 cctccaaaac gccagacctc cctgaacgtc attaaaccgt gatgttaccg actctctgac
     4621 gcggaacaga atcagcgtca gagaaacgga aaacgcgatc cagatcacaa atacattgta
     4681 ttcacatcat taaccgtttt aagatcattt catcactttt tcgcaactca cccgataatc
     4741 tgttatgaca acaaacactg tttcccgcaa agtggcgtgg ctacgggtcg ttacgctggc
     4801 agtcgccgcc ttcatcttca acaccaccga atttgtccct gttggcctgc tctctgacat
     4861 tgcgcaaagt tttcacatgc aaaccgctca ggtcggcatc atgttgacca tttacgcatg
     4921 ggtagtagcg ctaatgtcat tgccttttat gttaatgacc agtcaggttg aacggcgcaa
     4981 attactgatc tgcctgtttg tggtgtttat tgccagccac gtactgtcgt ttttatcgtg
     5041 gagctttacc gttctggtga tcagtcgcat tggtgtggct tttgcacatg cgattttctg
     5101 gtcgattacg gcgtctctgg cgatccgtat ggctccggcc gggaagcggg cacaggcctt
     5161 gagtttaatt gccaccggta cagcactggc gatggtctta ggtttacctc tcgggcgcat
     5221 tgtggggcag tatttcggtt ggcgaatgac cttcttcgcg attggtattg gggcgcttat
     5281 cacccttttg tgcctgatta agttacttcc cttactgccc agtgagcatt ccggttcatt
     5341 gaaaagcctc ccgctattgt tccgccgccc ggcattgatg agcatttatt tgttaactgt
     5401 ggtggttgtc accgcccatt acacggcata cagctatatc gagccttttg tgcaaaacat
     5461 tgcgggattc agcgccaact ttgccacggc attactgtta ttactcggtg gtgcgggcat
     5521 tattggcagc gtgattttcg gtaaactggg taatcagtat gcgtctgcgt tggtgagtac
     5581 ggcgattgcg ctgttgctgg tgtgtctggc actgctgcta cctgcggcga acagtgaaat
     5641 acacctcggg gtgctgagta ttttctgggg gatcgcgatg atgatcatcg ggcttggtat
     5701 gcaggttaaa gtgctggcgc tggcaccaga tgctaccgac gtcgcgatgg cgctattctc
     5761 cggcatattt aatattggaa tcggggcggg tgcgttggta ggtaatcagg tgagtttgca
     5821 ctggtcaatg tcgatgattg gttatgtggg cgcggtgcct gcttttgccg cgttaatatg
     5881 gtcaatcatt atatttcgcc gctggccagt gacactcgaa gaacagacgc aatagttgaa
     5941 aggcccattc gggccttttt taatggtacg ttttaatgat ttccaggatg ccgttaataa
     6001 taaactgcac acccatacat accagcagga atcccatcag acgggagatc gcttcaatgc
     6061 cacccttgcc caccagccgc ataattgcgc cggagctgcg taggcttccc cacaaaataa
     6121 ccgccaccag gaaaaagatc agcggcggcg caaccatcag tacccaatca gcgaaggttg
     6181 aactctgacg cactgtggac gccgagctaa taatcatcgc tatggttccc ggaccagcgg
     6241 tacttggcat tgctagcggc acaaaggcaa tattggcact gggttcatct tccagctctt
     6301 ccgacttgct tttcgcctcc ggtgaatcaa tcgctttctg ttgcggaaag agcatccgaa
     6361 aaccgataaa cgcgacgatt aagccgcctg caattcgcag accgggaatc gaaatgccaa
     6421 atgtatccat caccagttgc ccggcgtaat acgccaccat catgatggca aatacgtaca
     6481 ccgaggccat caacgactga cgattacgtt cggcactgtt catgttgcct gccaggccaa
     6541 gaaatagcgc gacagttgtt aatgggttag ctaacggcag caacaccacc agccccaggc
     6601 caattgcttt aaacaaatct aacattggtg gttgttatcc tgtgtatctg ggttatcagc
     6661 gaaaagtata aggggtaaac aaggataaag tgtcactctt tagctaacct tgcatcgcat
     6721 tgaccaaaac ttgaaccgat ttagcaaaac gtggcatcgg tcaattcatt catttgactt
     6781 atacttgcct gggcaatatt atcctctgca actaattact tgccagggca actaatgtga
     6841 aaagtaccag cgatctgttc aatgaaatta ttccattggg tcgcttaatc catatggtta
     6901 atcagaagaa agatcgtctg cttaacgagt atctgtctcc gctggatatt accgcggcac
     6961 agtttaaggt gctctgctct atccgctgcg cggcgtgtat tactccggtt gaactgaaaa
     7021 aagtgttgtc ggtcgacctg ggagcactga cccgtatgct ggatcgcctg gtctgtaaag
     7081 gctgggttga aaggttgccg aacccgaatg ataagcgcgg cgtactggta aaacttacca
     7141 ccagcggcgc ggcaatatgc gaacaatgcc atcaattagt tggccaggac ctgcatcaag
     7201 aattaacaaa aaacctgacg gcggacgaag ttgcaacact tgagcatttg cttaagaaag
     7261 tcctgccgta aacaaaaaag aggtatgacg atgtccagac gcaatactga cgctattacc
     7321 attcatagca ttttggactg gatcgaggac aacctggaat cgccactgtc actggagaaa
     7381 gtgtcagagc gttcgggtta ctccaaatgg cacctgcaac ggatgtttaa aaaagaaacc
     7441 ggtcattcat taggccaata catccgcagc cgtaagatga cggaaatcgc gcaaaagctg
     7501 aaggaaagta acgagccgat actctatctg gcagaacgat atggcttcga gtcgcaacaa
     7561 actctgaccc gaaccttcaa aaattacttt gatgttccgc cgcataaata ccggatgacc
     7621 aatatgcagg gcgaatcgcg ctttttacat ccattaaatc attacaatag ctagttgata
     7681 acgtgacaac gtcactgagg caatcatgaa accactttca tccgcaatag cagctgcgct
     7741 tattctcttt tccgcgcagg gcgttgcgga acaaaccacg cagccagttg ttacttcttg
     7801 tgccaatgtc gtggttgttc ccccatcgca ggaacaacca ccgtttgatt taaatcacat
     7861 gggtactggc agtgataagt cggatgcgct cggcgtgccc tattataatc aacacgctat
     7921 gtagttattg tcaggctctg atcacagagc ctgacatctc cgtaatatct tcctcaagtc
     7981 tcaaatattt tcgccattgt tggcgatgag atcccgatac cagtaaaacg acgctttacg
     8041 tatccgttcc agcgttccct tcccttcgtt gtctttatcg acataaatca ttccgtagcg
     8101 ctttttcatt tcccctgttc cggcagaaac caggtcaatg cagccccacg gggtgtagcc
     8161 aattaagtca acaccatctt caactacggc ttttttcatt tcccgaatat gggaagcaaa
     8221 gtaatcaatg cgatagtgat cgttcaccgt gccgtcagct tgtctctggt caaccgcacc
     8281 aaatccattt tcgacaataa acagcggcaa ctggaaatga tcccagaacc agttgagtga
     8341 ataacgtagc cctgccggat caatttgcca tccccattcg gatgtatcga tataagggtt
     8401 gctgaccaaa tctcgtggtt caacataatc cagttgcggg ttatcgggag aaaattgtgt
     8461 cgtaaaagac atgtagtagc taaaaccgat aaagtcgaca catccactgg caagaatcgc
     8521 gttatcttct ggtgtgatat cgaggttgaa tcctttcctg gcaaagtaat tcagcatatg
     8581 ttgcggataa tatccacgag catgaacatc agtaaaccag taacgacgat gcatcgcttt
     8641 cgtggccatc atcatatcgt tgggtgcaca cgtcagagga tagatggggc acatggcgat
     8701 catacagcca atgttaaatt caggattgat cgattttcca gtctgtaccg ccagggcact
     8761 ggcaactaac tcgtaatgta ccgcctggta cattatttgt tcgcgctcat tgatatcttc
     8821 ctctggcgaa tacaagatac cggaattagt aaatggacac aggctttcgc tgaaattcac
     8881 ctgattattg atttcgttaa acgtcatcca gtactttact ttttctttat agcgcgtgaa
     8941 gaccgttgat gcgaagcgga taaaaaagtc gatcagctta cggtttcgcc agccaccata
     9001 ttttgtcacc agatgataag gcatctcaaa atgcgaaagc gtcac
//
LOCUS       DAJVQG010000151         9003 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJVQG010000151 DAJVQG010000000
VERSION     DAJVQG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.151"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            1..1772
                     /locus_tag="OQM83_003349"
                     /pseudo
     CDS             1..1772
                     /locus_tag="OQM83_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1765..2901
                     /locus_tag="OQM83_003350"
     CDS             1765..2901
                     /locus_tag="OQM83_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCS1521536.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            2898..4898
                     /locus_tag="OQM83_003351"
     CDS             2898..4898
                     /locus_tag="OQM83_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCS1521537.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5020..5481
                     /locus_tag="OQM83_003352"
     CDS             5020..5481
                     /locus_tag="OQM83_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCS1521538.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5522..5992)
                     /locus_tag="OQM83_003353"
     CDS             complement(5522..5992)
                     /locus_tag="OQM83_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCS1521539.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6039..6758)
                     /gene="yehT"
                     /locus_tag="OQM83_003354"
     CDS             complement(6039..6758)
                     /gene="yehT"
                     /locus_tag="OQM83_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCS1521540.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(6755..8440)
                     /gene="btsS"
                     /locus_tag="OQM83_003355"
     CDS             complement(6755..8440)
                     /gene="btsS"
                     /locus_tag="OQM83_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCS1521541.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            8662..8727
                     /locus_tag="OQM83_003356"
                     /pseudo
     CDS             8662..8727
                     /locus_tag="OQM83_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
BASE COUNT         2204 a         2375 c         2298 g         2126 t
ORIGIN      
        1 cactcaatcg tcagcgcgaa gcctttatgg cccgctggat tggatgggcg atgcagcaaa
       61 acaatggcga cgtgttagtc gtctgcggtg gctggcacgc tcctgcactg gcaaaaatgt
      121 gacgcgaatg cccgcaggac attaacacgc cggaattgcc ctcgctggca gatgccgtta
      181 caggttgtta tctcaccccc tacagtgaaa agcgccttga tgtgctggca ggataccttt
      241 ccggtatgcc tgccccggtc tggcaaagct ggtactggca gtggggctta cagcaggcgg
      301 gagaacaact gctaaaaacg gttcttaccc gtttgcgcca gcacaaattg cctgcttcga
      361 ccgcggatat ggctgccgct catctgcatg cgatggcgct ggcacagttg cgcggacata
      421 aactaccgtt acgcactgac tggctggatg ccatagcagg ctcgctgatt aaagaagccc
      481 tgaacgcgcc gttgccgtgg agctatcgcg gagttattca tcccgacacc gatccgattc
      541 tgctaacgtt gatagacaca ttagcgggtg acggattcgg taaacttgcc ccttctacgc
      601 cacaaccgcc tctgccaaaa gatgtcacct gcgaactgga acgtaccgca atctctcttc
      661 cggcggagct taccttaaat cgctttaacc ccaatgggct agcgcaaagt caggtgttac
      721 atcggctggc aatactggag atcccaggga ttgtacgcca gcagggaagc acactgacac
      781 ttgcaggcaa cggtgaagaa cgctggaaat taacccgccc gcttagccag catgcggcat
      841 tgattgaggc cgcctgcttt ggtgccacac tccaggaagc cgcacgccat aaattagaag
      901 ccgatatgct ggacgcgggt ggaatcggca gtatcaccac atgtcttagc caggcggcgt
      961 tagcgggtct ggcgtccttc agtcaacaat tactggagca actcacatta ttaatcgccc
     1021 aggaaaatca atttgccgaa atgggccagg cgctggaagt gctatatgcc ttatggcggc
     1081 tggatgaaat tagcggtatg caaggcgcgc agatattaca aacgacgtta tgcgcggcta
     1141 tcgatcgcac gctgtggctg tgtgaatcta acggcagacc agatgaaaag gagtttcacg
     1201 ctcacctgca tagctggcag gcgctttgcc atatcctgcg cgatctacat agcggcgtta
     1261 atttacccgg cgtttttctt tctgcggcgg tagccttact ggagcgccgc agtcaggcga
     1321 ttcatgtccc ggcgctggat cgcggcgcgg ctcttggcgc actgatgcgt ctggaacatc
     1381 ccaacgccag tgccgaagcg gcgctgacga tgctggcgca gttatccccg gcacaatccg
     1441 gcgaggcgct gcacggtttg ctggcgctcg cccgtcatca actggcctgc caaccagcat
     1501 ttatcgccgg tttcagtagt catttaaatc aactgagtga cgacgatttt attaacgccc
     1561 tgcccgattt gcgcgcggcg atggcctggc taccgccacg agaacgcggg acactggcgc
     1621 atcaggtgct tgagcattac cagcttgcgc agcttcctgt ttcagcgctg caaatgccgt
     1681 tgcattgtcc tccgcaagcc attgcacatc atcaacaact cgaacagcag gcactggcat
     1741 cgctgcaaaa ctggggagtt ttccatgtct gaactgaacg atcttctgac cacccgtgag
     1801 ctacaacgct ggcgattaat tcttggcgaa gcggcagaaa cgacgctttg tgggctggat
     1861 gacaacgccc ggcagataga ccacgcgctg gaatggctgt atgggcgcga tcctgaacgg
     1921 ctccagcgtg gtgaacgctc cggtggatta ggtggctcaa atctcaccac tcctgagtgg
     1981 atcaacagta ttcacacgct gtttccgcaa caggtgattg agcggctgga aagcgatgcc
     2041 gtactgcgct acggcattga agatgtggtg acgaatctcg acatgctgga acgtatgcag
     2101 ccttctgaaa gcctgctacg cgccgttttg cacaccaaac atctgatgaa tcccgaagta
     2161 ctggctgccg cccgccggat agtgcgccag gttgttgaag aaattatggc tcgactggca
     2221 aaggaagttc gtcaggcttt ttctggtgtc cgcgatcgcc gtcgccgctc atttattcca
     2281 ctggcgcgaa actttgattt caaaagtact ctgcgcgcta acctgcaaca ctggcacccg
     2341 caacacggca agttgtatat cgaatccccc cgctttaaca gccgcattaa acgccaaagc
     2401 gaacaatggc aactggtctt actggttgat caaagcggat cgatggtgga ctcggtgatc
     2461 cactctgcgg tgatggcggc ctgtttgtgg cagttacccg gcattcgtac ccatctggtg
     2521 gcgtttgaca caagcgtggt tgatctcacg gcagacgttg ccgatccggt agagttatta
     2581 atgaaagtac agttgggcgg cgggaccaat atcgccagtg ccgtggagta tggtcggcaa
     2641 cttattgaac aaccagcaaa aagcgtcatt atcctcgtga gcgattttta cgaagggggt
     2701 tcatcatcat tgctgacgca tcaggtgaaa aagtgtgtcc agagcggcat caaagtgctg
     2761 ggactggctg cgctcgatag caccgcaaca ccttgctatg accgcgatac ggcccaggcg
     2821 ctggttaatg tcggcgcaca aatagccgcc atgacgccgg gcgagctggc atcatggctt
     2881 gcggagaatc ttcagtcatg aattcactac gtccggaatt attagaactg acaccgcagg
     2941 ccctgacggc gttaagcaat gccggttttg ttaagcgcag tcttaaggaa ctggaaaatg
     3001 gcaacgtccc ggagatcagc catgagaacg acgctttaat cgccaccttc agtgacggtg
     3061 tccgtaccca gctggcgaac ggccaggcac tgaaagaggc tcagtgcagt tgcggggcca
     3121 acggtatgtg ccgtcatcgc gtgatgctgg tgttaagtta tcaacgactt tgtgccacca
     3181 ctcagtctac ggaaaaagaa gaagagtggg atccggcaat ctggctggaa gaactggcta
     3241 cccttcccga tgctacccgc aaacgcgcac aggcgctggt cgctaaaggc atcaccattg
     3301 agttgttctg tgcgccgggt gaaattccct ctgcccgctt accgatgagc gatatgcgtt
     3361 tttattcccg cagcagtatt cgtttcgccc gttgtgattg tattgaaagc acactttgcg
     3421 aacatgtcgt actggcagta caggcctttg tccaggcaaa agcgcagcaa gcggaattga
     3481 ctcatttaat ctggcagatg cgcagcgaac acgtcacatc atctgacgat ccgtttgcca
     3541 gcgaagaagg caaaacgtgt cgtcaatatg ttcaacaact aagccaggca ttatggctga
     3601 gcggcatcag ccagccgctt attcactacg aggccgcttt cagccgcgcg cagcaagcag
     3661 cggaacgctg cagctggcga tgggtgagtg aatcgctacg gcaactgcgc gccagcgttg
     3721 atgccttcca cgcccgcgcc agccactatc atgccggaga atgcttacgt cagcttgcgg
     3781 cattaaacag tcgattaaat tgcgcacaag agatggcccg gcgcgacagt attggtgaag
     3841 ttcctcctgt gccgtggcgc acggtcgttg gctctggcat tgccggagaa gcaaagcttg
     3901 atcatctgcg gctggtgtct ttaggtatgc gttgctggca ggatattgag cattatggtt
     3961 tacgcatctg gtttaccgat cccgacaccg gcagtatttt gcacctttcg cgcagttggc
     4021 cgcgaagtga acaggaaaac tcaccggcag ctacgcgtcg gctgtttagt tttcaggctg
     4081 gcgcactagc gggcgggcaa attgtttcac aagcagcaaa acgcagtgcc gatggcgagc
     4141 tgctgttagc tacccgcaac cgcttaagca gcgttgtgcc gctgtcgcct gatgcctggc
     4201 aaatgttgag cgcgccgtta cgccagccgg gcattgtggc tttgcgggaa tatttacgcc
     4261 agcgtccccc cgcctgcata cggcctctta atcaggtcga taacttattt attctgccgg
     4321 tcgctgagtg tatttcgctc ggttgggata gcagccgcca gacgctggat gcgcaggtaa
     4381 tcagcggcga aggtgaagat aatctcctga cgttatccct tccggcatct gcctgttctc
     4441 cttttgccgt tgaacgcatg gcggcgcttt tgcaacaaac agacgacccc gtgagtctgg
     4501 tttctggctt tgtcagcttt gtagaggggc aattgacact ggaaccacgg gtgatgatga
     4561 caaaaacccg cgcctgggcg ctggacgcag aaactgcgcc tgtggcaccg ctaccttctg
     4621 ccagcgtttt gcctgtgccg tctaccgctc atcagttgct gatgcgctgc caggcgttac
     4681 ttattcaact gctccataac ggctggcgct atcaggaaca gagcgctatt agtcaggcag
     4741 agttgctggc gaatgacctc tccgcggtcg gtttttatcg gctggcacat gtgttgggac
     4801 aatttcgtaa tacagaaagc gaggcacggg tagaagcaat gaataacggt gttttacttt
     4861 gcgaacaatt attccccatg cttcagcaac aaggatgaaa tagtgcttac taagagttct
     4921 actccagttc cggactgctc acgccacggt attaggcata tcctatatag cccctggtga
     4981 gagtcaccag ttccttgatt aaataaaatg gagttttaca tgaaggcttt caataagctg
     5041 ttttccctcg ttgttgcatc tgttctggtt ttctctcttg ctggctgcgg tgacaaagaa
     5101 gaatcgaaga aattcagcgc caatctgaac ggcactgaaa ttgccattac ctatgtctac
     5161 aaaggtgaca aggtgcttaa gcaatcttct gaaaccaaaa ttcaatttgc ctccattggt
     5221 gcaaccacca aagaagatgc tgccaagaca cttgagccgt taagcgccaa atacaaaaac
     5281 atcgcgggtg ttgaagaaaa attaacctat accgatacct acgcgcagga aaacgtgact
     5341 atcgatatgg aaaaagtgga ttttaaagcc ctgcagggta tttcaggaat caacgtttct
     5401 gctgaagatg ccaaaaaagg tatcactatg gcgcaaatgg aactggtgat gaaagccgct
     5461 ggttttaaag aagtgaaata atctgtcggc ggccatgttt cgcatggccg ccatacccgt
     5521 cttagctttt cttcacatgc tggcgcgctg ccagtccacg cagaaaataa cgtaaaaatt
     5581 gatcgccgca ttcgcggaag tttttatgat ccggtgcgcg catcatcgct gtaatttccg
     5641 gcatcgaaac gcggaactgc tgttcggtga ggatcgccag aatgtcatcg gttttcagcg
     5701 aaaacgcgat gcgtaatttt ttcagcacga tgttgttatt aatgcgacgt tccggctcca
     5761 gtgccggagc agactcatcc ttgccgcgtt tttcataaat caggccattg aggaatgacg
     5821 acaaaacaat gtccggacaa cgctgaaaac cctcttcgtc ttctttacgt agccagacgg
     5881 cgatctgttc cgcggtggct tcgacattac ccagcgccag aatacgcacc aggtcattat
     5941 tattggcttt caaaatgtag cgcacgctgc gcagaatatc gttacttagc atgaggcctt
     6001 caggtgttaa tgaggcaaaa agccatttta gcagtctttt acaggccaat cgcctctttt
     6061 aagcttttca gataacggcg gctgaccggc acggttaagc cattacgcaa aatcaactcg
     6121 gcctggccgt tatcttccag acgaatctcc tgtaaatgcg cgaggttaac cagatactga
     6181 cgatggcagc gcagtagtgg tgtacgactt tccagggtac gtaatgtcaa ttcggtaaag
     6241 ccctctttcc cttcgtggct ggtaacgtag acaccgctca tccgactgct gacaaatgcc
     6301 acatctttca tttgcagcaa ataaatccga ctatgccccg tacaagggat aaatttcagc
     6361 gcctgttgat tttccggtaa cagcgaaaca tcctgcttgc tgcgctcctg acgcaatcgc
     6421 gccagcgttt tctccagtcg cgcttcatca attggcttca gcagataatc aaaggcatgt
     6481 tcttcaaagg ctttaatagc gtattcgtca aacgcagtga gaaaaacaat atacgggcga
     6541 tgttccgggt caagcatccc caccatttcc agaccactga tgcgcggcat ctggatatcg
     6601 agaaacagca catccgggcg cagtttatgc accgcgccga tcccttccac ggcgtttgaa
     6661 cactctccaa cgatttcaat atcgctctgc tcctgcaaaa atacacgcag gttctcccgt
     6721 gctaacggtt catcatcgac aattaagact ttaatcatgc ctcgtccctc catggtagtc
     6781 gtaacgttat tcgggtgtaa ctatcaggct cacaggcgac gcttattcca tagtcatcgc
     6841 caaaccgttc acgtaaacgc ttatccacca gattcatccc cagcccactg gcattggtta
     6901 ccggttgata caaaccggca ttgtcttcga tctccagcat caaatgttgc ccctcacgtc
     6961 gggcgctgat tgccactcgc cctgtatcca gcagttgtga tgtcccatgt ttaatggcgt
     7021 tttccactat cggttgcagg gtaaacgcgg gcaattgctg ctgggataat tcttgcggaa
     7081 tagcaatgtt gacctgcaac cgcgactgga agcgcgcctt ttcaatttgc agataagcgt
     7141 tcacatgttc aatttcgtcg gcgagagtaa caaactccga aggccgcttt aagtttttgc
     7201 ggaaaaaagt ggaaagatac tgcaccagct ggctggcctg ttcgctgtcg cggcggatca
     7261 ccgctttaat ggtgttaagc gcattaaaca aaaaatgagg attcacctgg gcgtgaagca
     7321 gtttgatttc tgactgggtg agcatcgctt tttgccgctc atattgcccg gcaagaatct
     7381 gcgccgaaag cagttgcgca atcccctcgc ccagcgtgcg gtttattgaa ctgaataaac
     7441 ggtttttggc ttcatacaat ttgatggtgc ccatcacgcg ctgattttcg ccacgcaacg
     7501 gaattaccag cgtcgacccc agtttgcatt gcggatgcaa agagcaacga taaggtactt
     7561 cgttgccatc agcgtagacc acttcaccgg tttcaatcgc ttttaaggtg taaggcgaag
     7621 aaatcggttt gccgggtaaa tggtggtcgt caccaattcc ggtaaaggcc agcaatttct
     7681 ctcgatcggt aatcgcgact gcaccaatat ccagctcctg atacagcacc tgagccactt
     7741 tcatgctgtt cacttcgtta aacccctgtc gcaaaatgcc ttccgtcgag gctgccactt
     7801 tcagcgcagt ggcagaaaaa gccgaagtgt atttttcaaa catcgcgcgt ttatcgagca
     7861 atatacgcat aaacagcgcc gcgccgacgg tattggtgac catcattggc gcagcaatat
     7921 tactcaccag acgcaccgca tcttcataag gtcgggcgat cgccaggatg atcagcattt
     7981 gcaccatttc agcgacgaac gtgacggcac cggcggtaat ggggttaaag actttatcag
     8041 tgcgcccgcg acggatcagg atgctgtgta ccaggccgcc gagcaatcct tcaacgatgg
     8101 ttgagatcat gcaacttagc gcggtcatgc cccccatcga atatcgatgt aagccgccgg
     8161 tcagaccaac cagcccaccg acgaccggac cgccgagtaa gccgcccatt accgcgccta
     8221 tcgcacgggt attggcaata gaatcgtcaa tgtgcaaccc aaaccaggtg cccatgatgc
     8281 agaagatgga aaagacgatg tagcagagaa atttatgcgg cagacgaacc gtgacctgca
     8341 ttaacggtat gaataatggc gttttactca ttaaccacgc aatgactaaa aaaacgcaca
     8401 tctgctgaag cagcagcaac accagattaa aatcgtacat acccgcaaac cacacttccc
     8461 tttaaaacgc gtaacataca ttgcctgcgt ttaactttct ttgaactctt gcagaaaaat
     8521 gagaattcgt gagtacgatc actcaaaatc gcctggcaaa aataaaatca ccctatagat
     8581 gcacaaaaaa cgggcaaaac tacctggttc gcaaaactgc gtctaaagtt aaaccgggac
     8641 ctcgcgagca agggtgagac gatggcgctt tacacaattg gtgaagtggc gttgctttgt
     8701 gatattaacc ctgtcacgtt acgcgcgtga cgcaacattt ttaaaaatcg acgaagatcg
     8761 tccaggagcg ccgtttttca agggttggat agatgattcg tttaccatgt cgacgaactt
     8821 cgacgaattt cgccgataaa aagtgtccca tacatgcccc attgatgccc cataaacaat
     8881 atcaggaatt gacactggtt atccatacag ataaaaataa cacctgtaca taaccacagt
     8941 tacaggaggg tgttatgcgt gttgagatct gtatcgctaa agaaaaaatc actaaaatgc
     9001 caa
//
LOCUS       DAJVQG010000152         8891 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJVQG010000152 DAJVQG010000000
VERSION     DAJVQG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8891
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.152"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            406..1281
                     /locus_tag="OQM83_003357"
     CDS             406..1281
                     /locus_tag="OQM83_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HCS1521542.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1289..2065
                     /locus_tag="OQM83_003358"
     CDS             1289..2065
                     /locus_tag="OQM83_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HCS1521543.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2234..4495
                     /locus_tag="OQM83_003359"
     CDS             2234..4495
                     /locus_tag="OQM83_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCS1521544.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            4564..5739
                     /gene="senB"
                     /locus_tag="OQM83_003360"
     CDS             4564..5739
                     /gene="senB"
                     /locus_tag="OQM83_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HCS1521545.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(6265..6726)
                     /locus_tag="OQM83_003361"
                     /pseudo
     CDS             complement(6265..6726)
                     /locus_tag="OQM83_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(6764..8302)
                     /locus_tag="OQM83_003362"
     CDS             complement(6764..8302)
                     /locus_tag="OQM83_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCS1521546.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPMEEALHGYVLTDGKLHVDDTPVPVLLPGNKKTKTGRLWAYVRDDRNA
                     GSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHA
                     RRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYADDGWAEADNNIAENALRMVSLGRKN
                     YLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVA
                     LPTE"
     gene            complement(8352..8699)
                     /gene="tnpB"
                     /locus_tag="OQM83_003363"
     CDS             complement(8352..8699)
                     /gene="tnpB"
                     /locus_tag="OQM83_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1521547.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT         2322 a         2204 c         2158 g         2207 t
ORIGIN      
        1 tttcgcacag tatgtggctg atacgcacac attaacgtac gttttcgttc cattggccct
       61 caccccccga taacgaccca ggtagcgcgc cgtattcccc ccgccggccg ttttcttcga
      121 catgtacatc aggcgcggaa ctcatcgggg cgcgggaatg aaatcccatt cagactccgg
      181 atagattcaa gcaagcccac ttatcgtcaa aatcggtgtt gcaaaaacgg gagtgaccat
      241 agatccgttt tccaagcgga ccccataata tcggctggta aacatcactc gctagtgcat
      301 tctttttgtt ggtaatgata atcatttaca ttcattcttt gatgttttat cataattaat
      361 gttttgtcaa taaatatatg gaaacatatt gtttcagatg ggaatatgag aaagttcatc
      421 cttatttcta tgataacgtt agctagtgtt agtctgggag cctgcagaca gaatgtcacc
      481 ataaagcagg atgcgccagg gcagaaagct ttcctgactg acgggcagat ttacgatttg
      541 cacagtggca aaattatttc tagcagcgag cttttggcgg atcttgctac cgctcgacat
      601 cttattattg gtgaaaaaca cgataatgcc gagcatcatc agattgagct ttggcttatt
      661 caaaatttgc tcatccagcg accacagggg agtgtgttgc ttgagatgtt gacgtcagag
      721 caacagcccc gagttaacca agttaaatgt tggctgaaag ataatcctgt ggttcgagat
      781 agccgtgttc aggaactact taactggcaa aaagggtggt cctgggagat gtatggcgac
      841 atcgtcatgc agctattacg tgggccttac ccgctgctca atgccaacat tggccgggag
      901 cagatgttgg cactgtacaa aaaaaacgaa tttcctaaag gaaaaaaatc cactgcacct
      961 gtggtacaag aagctctgcg tgaaaccatc atatctatgc atgaaggcaa ccttgaaagc
     1021 cagcaactga cgtcaatgct ctctattcag cagcagcggg atcgctacat ggcccgccag
     1081 cttctgagtg ccccggtacc gtcgttgttg attgcaggtg gttatcacgc gtcaaaaagc
     1141 atgggcgtac cgttacacat ggaagatctt gccaccggca cccatccagt ggtgttgatg
     1201 cttgcagaaa aaggaatgaa catcacagta gatcatgctg actacgtgtg gtttgtagcg
     1261 ccagatacaa caaaaaggta aaccatggat gatgaatatt ctccacttct cacagtcggt
     1321 aaaatggagc agctggttta tctgttctct actgcttcat ggactgattt tccttgcgtt
     1381 tatctggcgt ttttccgaag ttcagcccgc tatgtctcct gcaccggcga tcatgctgca
     1441 atgggcagaa attatagagg ctccttcttc tccactttcg cttccggtgg gaatcgctca
     1501 acaggagtcc gcagtgacag aagaaaagca acaaactgag gacagacaac aacgtcctgt
     1561 cactgaagac agtgatgcaa ccattgaaat aactaggaag aaaaagtcat ccgatggaga
     1621 aaagaaaaag acacgtccac ctcgtaagat aaaggctcaa accagcgaca gcaaccccac
     1681 agccgtttcc agtaatgctg cacctcaggc tctggtcgaa tcgtcccgta ttgctgcgcc
     1741 ttttaacagt gactcaacaa agcgagataa ttctgaggca tcttgggaaa gcagagtaaa
     1801 aggccaccta aaccgttata aacgttaccc tggcgatgcc agaaaacgtg cacgaaccgg
     1861 cacagccgtc gtgactttta ccgtaaacac ggaaggcact attgtcagta gtttcttgga
     1921 aatctcttca ggaacccttt ctcttgaccg cgaagctatt gctgttttag aacgcgctca
     1981 accacttccc aaaccaccgc ctgaaatatt ggagggagga ttatttaaag taaaaatgcc
     2041 gattaccttt aagttgaagg agtaaaggga taattggccg ttcaagttca tttaactgaa
     2101 atttaatttt catttttatg ctgaacattt tatcaaactt ttccactgtc gaaaaaatca
     2161 aaaagacagt agaacataat taataaacaa atagttatac ttatctttat taatattata
     2221 cccaaggaaa tttatgaacg ttataaaact ggctatcggc tcaggcatat tattgctcag
     2281 ctgcggtgct tactcacaat ccatcagtga aaaaactaat tccgacaaaa aaggagcggc
     2341 agaattcagt ccgctcagcg tttctgtcgg gaagacgacc agtgagcagg aagctctcga
     2401 gaaaacaggc gcgaccagtt cccggacaac ggacaaaaac ctgcaatcac ttgacgcaac
     2461 agtgcgtagt atgcctggta cttatactca aatagatcct ggtcagggag caatcagtgt
     2521 gaatattcga ggcatgagcg gatttggtcg tgtaaacact atggtcgatg gtattaccca
     2581 gagtttttac ggaacctcta cctccggaac aacgacgcat ggttcaacta acaatatggc
     2641 tggcgtactt atagatccta acttactggt agcagttgat gttacacgcg gtgacagcag
     2701 tggctctgaa gggatcaacg cccttgccgg tagtgcaaat atgcgtacta ttggcgttga
     2761 cgatgtaata tttaacggta atacatatgg ccttcgttca cgtttctctg tcggtagtaa
     2821 tgggctggga cgcagcggaa tgatcgccct tggtggaaaa agcgacgctt ttacggatac
     2881 gggaagcatt ggcgttatgg ctgctgtgag cggcagttct gtgtactcta atttctcaaa
     2941 tggttctgga attaacagca aagagtttgg ttatgataaa tatatgaagc agaaccccaa
     3001 atcccaactg tataaaatgg atatcagacc agacgaattt aacagcttcg aactttccgc
     3061 tcgaacctat gaaaataaat ttacacgtcg tgatataacc agtgacgact attacattaa
     3121 atatcattac accccttttt ctgaattaat tgactttaac gtaacggcca gtaccagtcg
     3181 cggtaatcaa aagtatcgtg atggctcgct gtatactttc tacaaaacct cagcgcaaaa
     3241 tcgttctgac gcgctggata tcaacaatac cagccggttc actgtcgcgg acaatgacct
     3301 ggagtttatg ctgggcagca aactgatgcg tacccgctat gaccggacca ttcactcagc
     3361 ggcgggcgac ccgaaagcga atcaggaatc gatcgagaac aatccgttcg caccctccgg
     3421 ccagcaggat atttcagcgc tgtataccgg gctgaaggtt acgcgcggca tctgggaggc
     3481 agatttcaat ctcaactaca cacgtaacag gatcacaggg tacaagcccg cctgcgattc
     3541 acgcgttatc tgcgtgccac agggtagcta cgatattgac gataaagagg gtggcttcaa
     3601 cccttcagtt cagctttctg ctcaggtaac accatggctt cagccgttca ttggctacag
     3661 caaatccatg cgcgccccga acatccagga gatgttcttc tctaattcag gaggcgcatc
     3721 catgaaccca ttcctgaagc ctgaacgtgc agaaacctgg caggcgggtt ttaacattga
     3781 taccagagat ttactggtcg aacaggatgc cctgcgcttt aaggctctgg cgtaccgcag
     3841 caggatccag aactacatct acagcgagtc ttatctggtt tgttctggag gtcgtaaatg
     3901 cagtatgcct gaggtgattg gcaatgactg ggagggcatt agcgatgaat acagcgacaa
     3961 tatgtacatc tacgttaact cggcaagcga cgttatcgca aagggcttcg aactggagat
     4021 ggattatgat gcaggttttg cttttggccg actctctttc agccagcagc aaacagacca
     4081 gccaacctcc atcgccagca cctactttgg cgcaggggat atgaccgaac tgcccagaaa
     4141 atacatgacg ctggatactg gtgttcgctt cttcgataac gcgttgaccc tgggcactat
     4201 cataaaatac acaggcaagg ctcgtcgcct gtcgcctgat tttgagcagg acgaacatac
     4261 cggcgcaata atcaaacagg atttgccgca gatcccaacg attatcgatc tctatggtac
     4321 ttacgagtac aaccgcaacc taacactgaa actttcggta caaaacctga tgaacagaga
     4381 ttattcggag gcgctgaata agctcaacat gatgccaggt cttggtgacg agacccaccc
     4441 agccaattcc gcgcgtggca gaacatggat atttggcggg gacattcgtt tctgatgaca
     4501 atttccgcta ggttcagaca atacgttttc tctcttatgt caattttatt gcaggaacga
     4561 aaaatgaata ttttcacttt atccaaagca ccgctatacc tgttaatttc actattttta
     4621 cccacgatgg ccatggctat cgatccacct gaacgcgaac tttcgcgatt tgccctgaaa
     4681 acgaattacc ttcagtcccc tgatgaaggc gtctatgaac tggcgtttga taatgccagt
     4741 aaaaaggtgt ttgcagcagt caccgatcgt gtaaatcgtg aagccaataa aggctatctg
     4801 tattcgttta attcagattc gctgaaagtc gaaaataaat acacgatgcc ataccgggca
     4861 ttttcgctgg cgataaatca ggataaacat cagctctata tcggacacac ccagtcagcg
     4921 tccctgcgta tcagtatgtt tgacacccca accggcaaac tggtaagaac cagcgacagg
     4981 ttaagtttta aagcggcaaa cgctgcagat tcgcgttttg agcattttcg ccatatggtt
     5041 tacagccagg attccgatac cctgtttgtg agttatagca atatgctgaa aacggccgag
     5101 ggcatgaagc ctctgcataa gctgttaatg ctcgacggga cgacgcttgc cttaaaaggc
     5161 gaggttaagg atgcttacaa aggtacagcg tatggtctga cgatggatga aaaaacacag
     5221 aaaatctacg ttggcggaag agattacatc aacgaaattg atgcgaaaaa tcagacgctg
     5281 ctgcgtacca tcccgttgaa agatccgaga ccacaaatca caagtgtgca gaatctggcg
     5341 gtggactccg cttctgaccg tgcctttgtg gtggtattcg accatgacga tcgttccggt
     5401 acaaaagatg gactctatat ttttgactta cgcgacggta aacagcttgg ctatgtgcac
     5461 acaggagccg gagctaacgc ggtgaaatac aatccgaaat ataacgaact gtatgtcacc
     5521 aacttcacta gcggcaccat cagcgtagtg gatgccacca aatacagcat cacccgtgaa
     5581 tttaacatgc cggtctaccc aaaccagatg gtgttgtcgg acgatatgga taccctttac
     5641 attggcatca aagaaggctt taaccgcgat tgggatcctg atgtgtttgt ggaaggagct
     5701 aaagaacgta ttctgagcat tgatttgaaa aagtcgtgat cggggggaaa aaacatggga
     5761 taaaaactcc gttattccac taaactggcc ggactaggaa aagcaaacat ctctgtcctg
     5821 ccggtacgcc tgttacgccg ctccatgagg ctgaggaatc tccagaaaac gagacaggca
     5881 cagcattgta cgatcaactg atttgtgcca ataattaaat gaaatcacag ctatgcctgt
     5941 tcatggggtt ctaggccgga accccagatt tttccgattg tttgttttct aactgattat
     6001 tcagtagttt tagagggctg acgctcagtg gaacgaaaac tcacgttaag aggttttgac
     6061 tatgataaaa gtcaaagtag gggtgagttt ggatatggaa attattgtac tataaggaga
     6121 gcatgaaaac ccctggggca gtcactgaat gagggaccag cagcggtaaa ataaccagca
     6181 taatttctgc atggtcgatt tttataccgt gacaggctgt tttttatact tggggtcagt
     6241 ttggatatcg aaaattattg tacgttaagg ttgttttttg accttgtcga tcttaactga
     6301 tctcaaacga cgaaacttac ctgaagtcgg tatccggctg tttttccaga tataatcgcc
     6361 tgtcagattg atatgttccc aacctaatgg tgataaatgc gataatagtt gagcatttat
     6421 tttcatgccc ttacgcttca gagcatcaac cgctctttcc atgtaaactg tgttccataa
     6481 ggaaatagcc gccgtcaata aagtcaatcc actggcgcga taactttgat tttccggttt
     6541 ccgatctcta atctccccca atcgatgcat gaatacagca cgggccagag cattacgggc
     6601 ttctcctttg ttcagcccgg cctggacacg tcgccgcaat gccggatccc gaaaccagtc
     6661 cagcataaac agtgttcgtt ctatacggcc aatctctctc agagctttag ccaacccatt
     6721 ttgtttgtaa gcgtgcagca agaaccgtat tgacgggaat gtgttattca gtcggcagtg
     6781 ctacgcgcca ggggagcagt tcgccgaccc ggtttatcgg ccagtcggct atgacgtcaa
     6841 ggacatagcg gaggtagctt tctggctcca ctccgttcag tttgcacgtc ccgatcaggc
     6901 tgtacagcag cgctccccgc tctcctccat gatccgaacc gaagaacagg tagtttttgc
     6961 ggcccagact gaccatccgc aacgcatttt cagcgatgtt attgtccgcc tcagcccagc
     7021 catcatctgc atagtacgtc agcgccggcc actggttcag ggcgtatgcg aacgctttcg
     7081 ccagttctga gtgtcgcgac agggttttca tcttttcacg cagccagctt tccagggatt
     7141 tcagcagcgg tttcgttttc aactgacgtt cggcaaggcg ctgttccgcc gtcattcccc
     7201 ttatctctgc ctcgatggcg tacagttcgc cgatccgttt cagcgcttcc tccgtcaggg
     7261 ctgacggggt gcgaacgtgc acatcgtgga ttttacggcg ggcgtgagcc caacaggcgg
     7321 cttccgttat ccggccatcc cggtacagct cgttgaaccc ggcgtatgca tccgcctgca
     7381 gtacaccact gaaccccgca agatgggtct gcggatggat gccttttctg tccgggctgt
     7441 aagcgaacca caccgccggc gccagcgttg acccggcgtt acggtcgtca cgaacatacg
     7501 cccacaaccg cccggtcttc gttttcttat tgcctggcaa cagcaccggg acaggcgtgt
     7561 catcaacatg gagcttaccg tcagtcagca catagccatg aagcgcctct tccatcggag
     7621 acagcagccg gcagcatgca tccacccagc ccgacagcag tgaacggctc agctccacac
     7681 cttgccggcc gtatatttct gactggcgat acagcggggt gtgctctgca tacttcgagg
     7741 tcagcacgcg ggccagcagc cccggtccgg cgataccccg ctcgatgggc cgcgaaggtg
     7801 caggtgcctg cacgatggca tcgcactgag tacaggcatg tttttcccgt accgtccgga
     7861 taacccggaa ggcgctacgc atcaactcca gctgttcggc ggtatcctcg cccagatagc
     7921 tcagtgaacc gccgcagttc gggcagcacg gcgccgcagg caacagtcgc ttttcgtcac
     7981 ggggtagtga ttcagggaac ggcttacggg tgcgggtctg acgcaacgga cgctgtactg
     8041 ccgggtcata caccctacca gtcagcgtat cgctctcttt ctgaagccgg ttcagatcgg
     8101 cttccatttg tgcgatacgg cgggagactt tttcggaacg actgccgaag ttcatccggc
     8161 ggagtttatc cagctgcgcc tgcagatggt ctatttcgcg ctcccggttg ctaagctttt
     8221 cctgcagggc gtggatcagc gcttcctgtt cggccaggcg ctgtttcagc aggaagatgt
     8281 cgtcagaaga gatgtcgttc ataagcccgt attttaccgg gcttattctg tgacaaccag
     8341 gataaagaga tttacagcat ggtcagggag gtcagcagcc gcttaggctg tcgccagtcg
     8401 ataccttcca gcagcatcgc cagctgcgcc tgtgtaagga acactttgcc atcacgggct
     8461 gacggccagg cgaagcgccc acgctccagc cgtttggtca ggaggcacag tccgtcactg
     8521 gtggaccaca gcagtttaac ctgactgccg ctgcggcccc ggaaaatgaa aacatggccg
     8581 gacatgggat cgtctttcag cgccgtctgt actttcgcag ccaggccgtt gaagccattt
     8641 ctcatatcgg tgataccggc aaccagccaa attttgatcc cggaaggtaa cgggatcatc
     8701 gcttcagttc ctgtatcagc agagtcagga gcttttcgct gacattgcca ttgaagcgga
     8761 gcgtcccgtg ccggaacgtt acctcacagc tgatactgag ggttttcggg tcctctgcga
     8821 gcgattctgg ctgttcggca gctgcatcga gagtcacagg aagtagctgg gggctctctg
     8881 aagaaggtaa t
//
LOCUS       DAJVQG010000153         8838 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJVQG010000153 DAJVQG010000000
VERSION     DAJVQG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.153"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            812..1054
                     /locus_tag="OQM83_003364"
     CDS             812..1054
                     /locus_tag="OQM83_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521548.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="OQM83_003365"
     CDS             complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="OQM83_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCS1521549.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="OQM83_003366"
     CDS             complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="OQM83_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCS1521550.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="OQM83_003367"
     CDS             complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="OQM83_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCS1521551.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="OQM83_003368"
     CDS             complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="OQM83_003368"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6320..6322),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCS1521552.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7101..7934
                     /gene="fdhD"
                     /locus_tag="OQM83_003369"
     CDS             7101..7934
                     /gene="fdhD"
                     /locus_tag="OQM83_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCS1521553.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            8087..>8838
                     /locus_tag="OQM83_003370"
     CDS             8087..>8838
                     /locus_tag="OQM83_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HCS1521554.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFK
                     QGPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQE"
BASE COUNT         2121 a         2232 c         2265 g         2220 t
ORIGIN      
        1 atttacatca ttccctgtct atgccggtaa tattgaataa gaactctata aatttttatt
       61 cttcgttggc accatacgtg tcttgctaca tcactgttca ggagcattta aatattattt
      121 gtagagctga gtggcaatta tagcagataa tgcttaactc atcatcttag ttcaggtttg
      181 agcgattttt gatagtcaaa acctcctctg gtgccatact tttctcataa atatttttaa
      241 aatctatagc tcacaaacat tatgcaaaga taatgggctg gcatcattgt cagtatcatt
      301 catttactgc tctgtatgtt atgaatcatt atgttgtatg tttgctctta aaccgtggga
      361 aggaggctat aatggttgcc aatattaatc tcattaaaca agaaagttat tctgttgtta
      421 atttagaaaa acaactttcc cgaactgtta ccaacaaaat tataacgatg agcaagtaaa
      481 gaccggttgg tggcagtact gaccggtctg tgatgaaaga ttaatttcac cttaaatcct
      541 ggtgctgtta ggatgctgac gtttttcggc tacaccattc agattatgca aataaatatc
      601 cctgaatata atatattcat agcacccata actcatcctg ggcgataact aaaccatttt
      661 tgagtaataa atgaaacaac tgcaacgctc ttattatttc tggaaaacgc ctcgcaaaaa
      721 taaaaaatga tgcgtaaatg agctttcttt aaagattcgg caagggtact tttctctcca
      781 tagattcaaa aatgatattg gcgagatatt tatgaactct ctggcaggta tagatatggg
      841 cagaatttta ctcgatttat cgaatgaggt gattaagcaa cttgatgatc tcgaggtgca
      901 gcgtaatctt cctcgtgcgg acctattaag ggaagcagta gatcaatacc tgataaatca
      961 atcgcaaaca gcaagaacca gtgttcctgg catctggcaa gggtgtgagg aagatggtgt
     1021 cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt caatcagatt
     1081 gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa
     1141 agtcatgcaa attcaatata ttgaagttta ttggtaggcc tgataagcgc agcgcatcag
     1201 gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt tggagggaag
     1261 aacgaggcaa acagacaatt gcggcatgat cttgaatggg taacgcggaa tacgagagat
     1321 ggtgtcgtca caccctatga gctgtaatcc gggcgacctg cgccacccgg aaaacgtcag
     1381 agattactct ccttcacccg gaaacaggaa cgggttaatg gaactacggg cgtagccttc
     1441 ttgctccatt cgcgcgtcca gtaccagaga ggcgaggtca tctgccacgg cttcaacttt
     1501 cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc agctttcggc
     1561 tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc tctgctcgca
     1621 gttgctgcat tttacgcgca ctacgtgcca ttcggtttca cacaggttgc agtgcaggta
     1681 acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag agccacatac
     1741 cgggcaatat tgacgttgtt cgccgtattc agcgcgggct ttgccgggga tcagattggc
     1801 catctgcgcc cagtagagcg acagcgcagc ccagataaac ggcgctttat cgctgctgac
     1861 ggacgagaaa tcagaggcaa acagcgcgct ggccatatct tccagctcct gagtcgatgc
     1921 cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt tcagctcagc
     1981 aattagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt gaatatccag
     2041 cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca tctccagcgg
     2101 atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc gcaggtaatc
     2161 acccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt tgtataaatt
     2221 cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct cgctcgaacc
     2281 cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt tgtcttgcgg
     2341 acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac ccatccttcc
     2401 accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac catgataacc
     2461 acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat caccgggatt
     2521 gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag cagcagaacc
     2581 aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg cccggtgtca
     2641 cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat atcatcccga
     2701 ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc aaacataacc
     2761 acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat ttgcatcaac
     2821 cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgccaggat gaagcagaag
     2881 gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt gtcacgtcgt
     2941 ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca tccgcacggt
     3001 tcggaccgac acccacgtag tggaagatac tggccgcaaa ggtagccgca aagccaacag
     3061 ccgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt tccgggttct
     3121 ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac atgacgtgtg
     3181 taccaccgac gcctgccgga tcgtacagac ccgcattgtc gtaaccgcgg gttttcagct
     3241 cagcaacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag tgaatcgcgc
     3301 ccgttgggca ggtcttcacg caggctggtt cttgcccaac caccacgcgg tcaacgcaca
     3361 gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg aacggacagc
     3421 ccgcaatgca ataaccgcag ccaatgcact gctcggactg gaagtcgacg ataccgttgg
     3481 catactgaat aattgcacct tccgccgggc acgctttcag gcagcctgga tcggaacagt
     3541 gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc acttccgaga
     3601 agcgcatcac cgtccacgat ttggcgctta aatcattggg gttgtcgtac accccaatgt
     3661 tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga caggctttac
     3721 agccgataca ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag tcccgcgcct
     3781 gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttgc gattgataag
     3841 ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta aactccggcg
     3901 tctgcgtgtt cgcatcacca acgaatggcg ttaacgtatt ggcaataaag ccttttttag
     3961 ctacgccttc atagccccag tgaatcggaa taccgatggt atcgatatct ttgccgttcg
     4021 ctttcagcgt gcgaatacgt ttggtcacca ccgctttggc tttgatatag ccacggttgg
     4081 aggagacttt cacggtatcg ccctgggcaa tgccaagttt attcgccagc gactccccga
     4141 tttccacaaa ctgctctggt tgcaaaatcg cgttcaacag cgcgtgtttg gtccagtagt
     4201 ggaagtgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg gctttaccca
     4261 atgcttcggc gtcgtcttta aagattcgcg cagccgggtt cgagataacg tttggatgca
     4321 gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac ggaccttccg
     4381 ccatcttatc gagggcaaac agacgcccca tgccttcctg ctgcatgata aacggcccga
     4441 cgccgctgcc cggaggcgct gcgctgtagt ccggaatatc ccagccggtc cacttagtgc
     4501 cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga tctgcggagg
     4561 cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc agcgtgttac
     4621 cgaggccaga cggatcggcg ttatcacgac gcgccatctg gttgccttcc ggcgtccagc
     4681 taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat tgggcgaacg
     4741 agctaagttg ttggcctttc ttgacgataa ccgcaccggt tgccggatcg gtaatatcgg
     4801 ccagcgcctt accgttgctc tccatcgcca cttcttccga cttcggctca tgcggaatgg
     4861 cgtagttcca ggtcatgttc agcacctgat ccgggtttgc gccaccctgt tcggcataca
     4921 tcttgcgcaa gcgcaggaag ataccggaga ggatctcgcc atcagtcagc gcaatccctg
     4981 gggcgtccgc acctttccag tgccactgca accagcgacc tgagttaacg attgaaccgt
     5041 tctcttctgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc ttcgacgagt
     5101 caacttcgtt cagctcaccg tggttctgcc agaagttaga ggtttcagtg ttcagcgggt
     5161 cgatggtgac gaggaacttc agtttcgaca gacaaccgat cactttgttt ttgttcggga
     5221 atgaggcaac agggttaaag ccctggcaga tatagccatt gaccttgccc tctttcatca
     5281 tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag tcaaagcccc
     5341 agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcatagag acgaagaatt
     5401 tcgggtagtt gccccagtag tttacctggc cttccagcag cggttttggc gtgttggcgg
     5461 taaggtaggt ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa cctggcaggc
     5521 tctgcgacag cagccccagg tccgtcagcc cctgaatatt ggagtgaccg cgcagggcgt
     5581 taacgccgcc gcctgccatc cccatattgc cgagcagcag ctggatcatc gccatcgtac
     5641 gaatgttttg cgcaccaacg gagtgttgcg tccagccgag ggcatacagg aacgaggcag
     5701 ttttatcgtg agcactggtt tctgcgatgt attcgcagac tttcaggaac gcgtcttttg
     5761 gcgtaccaca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc tgtttcagca
     5821 agttccacac gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag ccgttttcgt
     5881 ccagttcgta agtccaggag gatttatcgt acttgcgttt ttccgcgtca tagccggtga
     5941 acaggccatc ttcaaagccg taatcctcac gcacgatcag gctggcgttg gtataggctt
     6001 cggtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg cctgacagga
     6061 aagcaatgtc agtaccggaa cgaatagggg catagtagtc agccaccgcc gccgtacgcg
     6121 taaagcgagg atcgatcaca atcagcttcg cgccattgtg aattttggct tccatcgccc
     6181 agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg acgaggttgg
     6241 cgttcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt ggagcaagac
     6301 ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc atacccagcg
     6361 cgcgggagaa tttttgcgtt aaatagccgg tttcgttact cgacgcggaa gcacacagca
     6421 tcccggtgga gagccagcgg ttaacagtca cgccttcggc gttttgcgca atgtagttag
     6481 catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc caactgattt
     6541 gctgccattt atcagaacct ggcgcacggt attccggaaa cttcagacgg ctttcggagt
     6601 ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc accgcggttg accgggtgat
     6661 ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg tcaccgaggc
     6721 tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg gtttcgcggg
     6781 tgcgcagcag tttatactgc cgggtttccg cgagtgctac gctgggtgca aaacccagtg
     6841 ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc cttctgctga
     6901 cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc gcttgctgcg
     6961 tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc cagaattggc
     7021 tcaatttccc tccatccagg aagggtttgt aacagaatac cataatgttg gtgtgtgtgt
     7081 tcttatctgg ttaagagaaa gtgaaaaaaa cacagcgaaa agaaatcgaa aatgtgacaa
     7141 atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa cacccacggt
     7201 tagatgaggt cgcggaagaa gttcccgttg cgctggtcta caacggcatt tcgcatgtgg
     7261 tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg ctttccgagg
     7321 ggataataga aagtccgcgc gatatcttcg gcatggatgt cgttccttcc tgtaatggtc
     7381 ttgaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggag cgccgccggg
     7441 cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat gacatcggaa
     7501 aaccggtgca gccgctaccg ttcacccaga cgtttgatct caacaaactg gatgatgcat
     7561 tacgtcatct caacgatttt cagccagtgg ggcaactgac tggttgtact cacgccgctg
     7621 cctggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggt cgccatgtgg
     7681 cgctggacaa actgttaggc cgccggtcac aagaagggga aagctggcag caaggtgcgg
     7741 tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg tgcggcgtag
     7801 agattttgtt tgcggtgtct gccgcgacca cgcttgctgt agaagtggct gagcgctgta
     7861 atctgacact ggtaggtttt tgtaaaccgg gtcgggcaac ggtttatacc catccgcagc
     7921 gtttgagcaa ttaattaaaa taagtcctgc gatatatatt aaattgcagg acttattcat
     7981 ttcgtgaatt ttattatttt atttataaat aaaacgcgtg tagtgacgtt ttcttttttc
     8041 atttattatg ttcactctct tatctatata tttataaggc aattaaatga aaaggaattt
     8101 attatcttcc gctattatag tcgccatcat ggccctcggt ctgacgggtt gtgatgataa
     8161 aaaagccgaa acagaaacgc ccccgcctgc caatagtcaa cctgccgcac cagctcctga
     8221 agctcccgtt gcaaaagcag aagctaaacc tgaaacacct gcgcaaccgg tggtcgatga
     8281 acaagcggtt ttcgacgaaa aaatggatgt ctatatcaag tgctacaaca agttacagat
     8341 ccctgtacag aacagtctgg cgcgttatgc tgactggctg aaagatttta aacaggggcc
     8401 taccggtaaa gagagtactg tttatggcat ctacggcatt agtgaatcca acctcgctga
     8461 gtgtgaaaaa ggcgtaaaaa gtgctgtggc gttaacgcct gcgctgcaac ctattgatgg
     8521 cgtggcggtg agttatattg atgctgccgt ggcgttgggt aataccatta acgaaatgga
     8581 taaatattac acccaggaaa attataaaga cgatgcgttt gcgaaaggga aaacgctgca
     8641 ccagacattc ttaaaaaatc tggaatcctt tgaacctgta gcagaatctt atcatgcggc
     8701 gattcaggaa attaatgata agcgccagct tgcggaactg aaaaatattg aagaaagaga
     8761 agggaaaaca ttccactact actctctggc agtcatgatt tcagcgaaac aaattaataa
     8821 cctgatttcg caagagaa
//
LOCUS       DAJVQG010000154         8725 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJVQG010000154 DAJVQG010000000
VERSION     DAJVQG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8725
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.154"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..1024
                     /gene="rhaB"
                     /locus_tag="OQM83_003371"
     CDS             <1..1024
                     /gene="rhaB"
                     /locus_tag="OQM83_003371"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCS1521555.1"
                     /translation="LIPHIAHALLMPDYFSYRLTGKMNWEYTNATTTQLVNINSDDWD
                     ESLLAWSGANKAWFGRPTHPGNVIGHWICPQGNEIPVVAVASHDTASAVIASPLNGSR
                     AAYLSSGTWSLMGFESQTPFTNDTALAANITNEGGAEGRYRVLKNIMGLWLLQRVLQE
                     RQINDLPALIAATQALPACRFIINPNDDRFINPEAMCSEIQAACREMAQPLPESDAEL
                     ARCIFDSLALLYADVLHELAQLRGEDFSQLHIVGGGCQNTLLNQLCADACGIRVIAGP
                     VEASTLGNIGIQLMTLDELNNVDDFRQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQ
                     TKELCA"
     gene            1021..2280
                     /gene="rhaA"
                     /locus_tag="OQM83_003372"
     CDS             1021..2280
                     /gene="rhaA"
                     /locus_tag="OQM83_003372"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCS1521556.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQAIGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            2522..3346
                     /gene="rhaD"
                     /locus_tag="OQM83_003373"
     CDS             2522..3346
                     /gene="rhaD"
                     /locus_tag="OQM83_003373"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCS1521557.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            3356..3670
                     /gene="rhaM"
                     /locus_tag="OQM83_003374"
     CDS             3356..3670
                     /gene="rhaM"
                     /locus_tag="OQM83_003374"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCS1521558.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            3971..4417
                     /locus_tag="OQM83_003375"
     CDS             3971..4417
                     /locus_tag="OQM83_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCS1521559.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            4428..5879
                     /locus_tag="OQM83_003376"
     CDS             4428..5879
                     /locus_tag="OQM83_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCS1521560.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            5869..6939
                     /locus_tag="OQM83_003377"
     CDS             5869..6939
                     /locus_tag="OQM83_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCS1521561.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVRHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            6939..8687
                     /locus_tag="OQM83_003378"
     CDS             6939..8687
                     /locus_tag="OQM83_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HCS1521562.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
BASE COUNT         2021 a         2305 c         2340 g         2059 t
ORIGIN      
        1 acttattcca cacattgctc acgctctgct gatgccggat tacttcagct atcgcctgac
       61 cggcaagatg aactgggagt acaccaacgc cacgaccacg caactggtca atatcaatag
      121 cgacgactgg gacgagtcgc tactggcgtg gagcggggct aacaaagcct ggtttggtcg
      181 cccgacgcat ccgggtaatg tcattgggca ctggatttgc ccgcagggta atgagattcc
      241 ggtggtcgcc gttgccagcc atgataccgc cagcgcggtt atcgcctcgc cgttaaacgg
      301 ttcacgcgct gcttatctct cttctggcac ctggtcattg atgggcttcg aaagccagac
      361 gccatttacc aatgacacgg cgctggcagc caacataacc aatgaaggcg gggcggaagg
      421 tcgctatcgg gtgctgaaaa atattatggg cttatggctg cttcagcgag tgcttcagga
      481 gcggcaaatc aacgatctcc cggcgcttat cgccgcgaca caggcacttc cggcctgccg
      541 cttcatcatc aatcccaatg acgatcgctt tattaatcct gaggcgatgt gcagcgaaat
      601 tcaggctgcg tgtcgggaaa tggcgcaacc gctcccggaa agtgatgctg aactggcgcg
      661 ctgcattttc gacagtctgg cgctgctgta tgccgatgtg ttgcatgagc tggcgcagct
      721 acgcggtgaa gatttctcgc aactgcatat tgtcggcggc ggctgccaga acacgctgct
      781 caaccagcta tgtgccgatg cctgcggtat tcgggtgatc gccgggcctg ttgaagcctc
      841 gacgctcggc aatatcggca tccagttaat gacgctggat gaactcaaca atgtggatga
      901 tttccgtcag gtcgtcagca ccaccgcgaa tctgaccacc tttaccccta atcctgacag
      961 tgaaattgcc cactatgtgg cgcagcttca ctcgacacga cagacaaagg agctttgcgc
     1021 atgaccactc aactggaaca ggcctgggag ctggcgaaac agcgtttcgc ggcggtgggg
     1081 attgatgtcg aggaggcgct gcgccaactt gatcgtttac ccgtttcaat gcactgctgg
     1141 cagggcgatg atgtttccgg ttttgaaaac ccggaaggtt cgctgaccgg tgggattcag
     1201 gctattggta attatccggg caaagcgcgt aatgccagtg agctacgagc cgatctggaa
     1261 caggctatgc ggctgattcc ggggccgaaa cggcttaatt tacatgccat ctatctggaa
     1321 tcagacacgc cagtctcgcg cgaccagatc aaacctgagc acttcaaaaa ctgggttgaa
     1381 tgggcgaaag ccaatcagct cggtctggat tttaacccct cctgtttctc gcatccgcta
     1441 agcgccgatg gctttacgct ctcgcatccc gacgacagca ttcgccagtt ctggattgat
     1501 cactgcaaag ccagccgtcg cgtttcggcc tattttggcg agcaactcgg cacaccatcg
     1561 gtgatgaaca tctggatccc ggatggtatg aaagatatca ccgttgaccg tctcgccccg
     1621 cgtcagcgtc tgctggcagc actggatgag gtgatcagcg agaagctgga tccggcgcac
     1681 catatcgacg ccgttgagag caaattgttt ggcattggcg cagagagcta cacggttggc
     1741 tccaatgagt tttacatggg gtatgccacc agccgccaga ctgcgctgtg cctggacgcc
     1801 gggcatttcc acccgactga agtgatttcc gacaagattt ccgccgccat gctgtatgta
     1861 ccgcagttgc tgctgcacgt cagccgtccg gttcgctggg acagcgatca cgtggtgctg
     1921 ctggatgatg aaacccaggc gattgccagt gagattgttc gtcacgatct gttcgaccgg
     1981 gtgcatatcg gcctcgactt ctttgatgcc tctatcaacc gcattgccgc gtgggtcatt
     2041 ggtacacgca atatgaaaaa agccctgctg cgtgcgttgc tggaacctac cgctgagctg
     2101 cgcaagctgg aagcggcggg cgattacact gcgcgtctgg cgctactgga agaacagaaa
     2161 tcgttgccgt ggcaggcggt ctgggaaatg tattgccaac gtcacgatac gccagcaggt
     2221 agcgaatggc tggagagcgt gcgggcttat gagaaagcga ttttgagcca gcgtgggtaa
     2281 acactgccgg atgcggcgcg agcgccttat ccggcctaca ggtcgaatga ccatgcgtgc
     2341 tgccaccctc accccggccc tctccctgaa agggcgaggg agaaaaacat agcctcgtgc
     2401 ggcaacggag ctgaacaccg ctccggacag tcccctcgcc cctacgggga gagggttagg
     2461 gtgaggggaa caagccgcac catgtctaaa caaggcaacc taatttacag gaacacagaa
     2521 catgcaaaac attacccagt cctggtttgt ccagggaatg atcaaagcca ccaccgatgc
     2581 ctggctgaaa ggctgggacg agcgcaatgg tggtaacctg acgctgcgcc tggatgacgc
     2641 cgatatcgcg ccttatcacg gcaactttca tgcacaaccg cgctatattc ccctcagcca
     2701 acccatgcct ttactggcaa atacgccgtt tattgtcacc ggctccggca aattcttccg
     2761 taacgtccag cttgatcctg cagctaactt aggcgtcgta aaagtcgaca gcgacggcgc
     2821 gggctaccat attctctggg ggttaaccaa cgaagccgtt ccaacttccg aacttccggc
     2881 tcacttcctt tcccactgcg agcgcattaa agccaccaac ggtaaagatc gggtgatcat
     2941 gcactgccac gccaccaacc tgatcgccct cacctatgta cttgaaaacg acaccgcggt
     3001 ctttactcgc caactgtggg aaggcagcac cgagtgtctg gtggtattcc cggatggcgt
     3061 tggcattctg ccgtggatgg tgcccggcac ggacgaaatc ggccaggcga ccgcacaaga
     3121 gatgcaaaaa cattcgctgg tgttgtggcc cttccacggc gtcttcggca gcgggtcgac
     3181 gttggatgaa acattcggtt taatcgacac cgcagaaaaa tcagcacaag tattagtgaa
     3241 ggtttattcg atgggcggca tgaaacagac catcagccgt gaagagttga tagcgctcgg
     3301 caagcgcttc ggcgttacac cactagctag tgcgctggcg ctgtaaggag caaacatgat
     3361 ccgcaaagcc tttgtcatgc aggttaaccc cgacgcccac gaagagtatc agcgtcggca
     3421 taaccccatc tggccggaac tggaagcagt gttgaaatct cacggtgcgc ataactacgc
     3481 catttacctc gacaaagcgc gtaatctgct gtttgccacg gtagagattg aatctgaaga
     3541 acggtggaat gcggtagcca gcactgatgt ttgccaacgt tggtggaaat atatgaccga
     3601 tgttatgccc gctaacccgg ataacagccc ggtgagtagc gagctgcaag aagtgtttta
     3661 cctgccgtaa ttgcccaatg tgccagagat gcctccggca caatatttta aatttcgatt
     3721 tgaaaaatta aacatattac atctcccggt aagttatatt tccctgatac attgtgagta
     3781 aatcacaaaa ataatgaata acccattaat gattcatgtg gtttatttaa ataaccatta
     3841 tgtgcattac tccgcaaatc tgacctttca ctctgtctca aattgtcatt gtttaccgac
     3901 atatcgcctc tcatcactta atcgctccac cattacaatt gattaaaata tattttatgg
     3961 agtccgatta atggcagctc ttactgcaag ctgtattgac ctgaatattc agggcaatgg
     4021 cgcttattcc gttctgaagc agttggcgac aatagcgtta caaaacggtt ttatcaccga
     4081 ctcacaccag ttcctgcaaa ccctgctgct gcgcgaaaaa atgcactcta ctggatttgg
     4141 ttccggtgtc gccgtgccgc acggtaaaag cgcctgcgtt aaacaaccgt ttgtattatt
     4201 cgcccgcaaa gcgcaggcta ttgactggaa agccagcgat ggcgaagacg tcaattgctg
     4261 gatctgcctc ggcgtgccgc aaagcggcga agaggatcag gtcaaaatca tcggcacact
     4321 gtgtcgcaaa attatccacc aggattttat tcatcaactg caacagggcg acgccgacca
     4381 ggtgcttgcc ttgttaaatc aaaccctcag ctcataagga agtggcgatg gagtcatcct
     4441 tatgtattgt cgcgatcacc aactgccccg ccgggatcgc tcacacctac atggtggcgg
     4501 aagccctgga acagaaagcc cgttctctcg gccataccat aaaagtggaa actcaagggt
     4561 ccagtggcgt tgaaaaccgc ttatccagcg aagagattgc cgctgccgat tacgtcattc
     4621 tcgctaccgg gcgtggcctg agcggtgatg atcgcgcgcg gtttgccggg aagaaagttt
     4681 atgagattgc catctcccag gcgttgaaaa atatcgacca gattttcagc gaattaccga
     4741 caaactcgca gctttttgcc gcagatagcg gcgtgaagct tggcaaacag gaagtgcaaa
     4801 gcggcagcgt gatgagccac ctgatggcgg gcgtctctgc ggcgctgccg tttgtcatcg
     4861 gcggtggtat cctggtggcg cttgccaaca tgctggtgca gttcggtttg ccgtataccg
     4921 atatgtcgaa aggcgcgccg tcgtttacct gggtagttga atccatcggt taccttggct
     4981 tcacctttat gatccccatc atgggcgctt atattgcctc gtcgattgcc gataaaccgg
     5041 ctttcgctcc ggcgtttctg gtttgctatc tggcgaacga caaagcgctg ctcggcaccc
     5101 agtcgggtgc gggattcctc ggcgcggtgg tgctggggct ggcgattggc tatttcgtct
     5161 tctggttccg caaagtgcgc cttggcaaag cattacaacc gctgctcggt tcaatgctga
     5221 tcccgttcgt tactctgctg gttttcggcg tgctcactta ctacgttatc gggccggtga
     5281 tgtccgacct catgggcggg ctgctccact tcctgaatac cattcctcca tcaatgaagt
     5341 ttgcggcagc gtttctggtc ggtgcgatgc tggcgttcga tatgggcggc cccatcaata
     5401 aaaccgcctg gttcttctgc ttctcactgc tggaaaaaca catttatgac tggtacgcca
     5461 tcgtcggcgt tgtcgcgttg atgccgcccg ttgcggcggg tcttgcgacg ttcattgcgc
     5521 ccaaactgtt tacccgacag gaaaaagagg ctgccagtag tgccattgtg gtcggtgcca
     5581 cagtcgcaac cgaaccggct ataccttacg ctctggcagc tccgttgccg atgattaccg
     5641 ccaatacgct ggcgggtggc attaccggcg tgctggtgat cgctttcgga atcaaacgtc
     5701 tggcacctgg tttgggtatc tttgacccgc tgatcggcct gatgtcgccg gtgggttcgt
     5761 tttatctggt gctagcgatt ggcctcgcgc tgaacatttc attcattatc gtcctgaaag
     5821 gattgtggtt acgccgcaaa gcgaaagccg cgcaacagga gcttgtccat gaacattgag
     5881 ttactgcaac agttgtgcga agccagcgcc gtcagcggcg atgaacagga agttcgcgac
     5941 attctgataa acacgctgga accttgcgtt aatgagatca cctttgatgg tctgggcagc
     6001 tttgttgccc gtaaggggaa taaaggtcca aaagttgccg ttgtcaggca tatggatgaa
     6061 gtcggcttta tggtcaccca catcgacgag agcggttttc tgcgctttac caccattggc
     6121 ggctggtgga atcagtcaat gctcaaccac cgggtaacca tacgcacaca caagggattt
     6181 aaaatccctg gtgtgattgg ttccgtcgca cctcatgcgt taacggaaaa gcaaaagcaa
     6241 cagccgctgt catttgatga gatgttcatt gatattggcg cgaacagtcg cgatgaggtg
     6301 gaaaaacgcg gtgtggcgat gggcgatttt attagcccgg aagccaattt tgcctgctgg
     6361 ggcgaagata aagtggtcgg caaggcgctg gataaccgca tcggctgcgc aatgatggct
     6421 gaactattgc agacggtgaa taatcccgaa attacgctgt atggcgtcgg cagcgtggaa
     6481 gaagaagttg ggctacgcgg ggcacaaacc tcggcggaac acattaaacc ggatgtggtg
     6541 attgtgctgg ataccgctgt cgcaggtgat gttccgggca ttgataacat taaatacccg
     6601 ctgaaactgg gccaggggcc ggggctgatg ctgtttgaca agcgctactt ccccaaccag
     6661 aaactggtgg cggcgttaaa aagctgtgcc gcacataacg atttaccgct gcaattttcc
     6721 accatgaaaa ccggtgcgac ggatggcggg cgctacaacg tgatgggcgg cgggcgtccg
     6781 gttgttgcgc tgtgcctgcc aacccgctat ctgcacgcta acagcggcat gatttcaaaa
     6841 gccgattacg atgctctgct cacgttgata cgggattttc tgaccacctt aaccgcggag
     6901 aaagtcaacg cgtttagcca gttccgtcag gtggattaat gctaaacgaa cgccagttaa
     6961 agattgtcga tctgctggag caacagccgc gcacgcctgg tgagctggcg caacagactg
     7021 gcgtttcagg caggaccatc ctgcgtgata ttgactatct caactttacc cttaacggca
     7081 aagcccgcat tttcgccagt ggcagtgcgg gctatcagct ggaaatcttc gagcgccgca
     7141 gcttttttca gttgctgcaa aagcacgata acgacgatcg gctgctggcg ctgttattac
     7201 tgaatacctt tactccccgt gcgcaactcg cctcggcgct taatttgcca gaaacctggg
     7261 tagcagagcg tctgccccgg ttaaaacagc gttatgaacg cacttgttgc ctggccagtc
     7321 gccccggttt gggccatttc attgatgaga cagaagagaa acgcgttatc ttgctggcga
     7381 acttgctgcg caaagatccg tttttaattc cgctggcggg cgtaacacga gacaattttc
     7441 agcatttatc cacggcctgc gacaaccaac accgctggcc gctcatgcag ggtgattatc
     7501 tctccagcct gattctggcg atttacgccc tgcgtaatca actgaccgat gagtggccgc
     7561 aatatcccgg taacgagata aaacaaatcg ttgaacagag cggcatgttt cttggtgata
     7621 acgctgtaag aaccctgaca ggtttgatag agaaacagca tcagcaagcg caggtaattt
     7681 cagccgatca tgtgctgggg ttgctgcaaa gggtgccagg catcgcgtca ttgaatatta
     7741 ttgatacgca gctggttgag aatattaccg ggcatttatt acgttgcctt gccgcaccgg
     7801 tgtggattgc cgagcaccgc caaagcagca tgaataacct gaaagccgcc tggcctgcgg
     7861 cctttgatat gagtctgcac tttattacgc tactgcgtga acagctcgat attccccttt
     7921 tcgatagcga tctgatcggt ttgtattttg cctgtgcgct ggaacggcat caaaacgaac
     7981 gccagccgat cattttgctc tcggaccaga acgcgattgc cactattaat cagctcgcca
     8041 ttgagcgcga tgttttacat tgtcgggtga ttattgcccg tagcttaagc gagcttattg
     8101 ccattcgcga agagattgag ccgttattga tcattaacaa cagccattat ttactggatg
     8161 acgcggtaaa taattacatc accgtaaaaa atatcattac ggctgccgat atcgaacaaa
     8221 taaaacactt tctggcgacg gcatttattc gccaacagcc ggaacgcttt ttctctgccc
     8281 ccggaagttt tcattattcg aatgtacgcg gtgaaagctg gcaacatatt acccggcaaa
     8341 tttgtgcgca attagtggca caacaccata ttaccgccga tgaagcgcaa cggatcatcg
     8401 cccgcgaagg cgaaggtgaa aacctgattg ttaatcgcct cgccatccca cattgctgga
     8461 gcgaacagga gcgacgtttt cgtggatttt ttattaccct cgcccaacca gttgaggtga
     8521 ataacgaagt cattaaccat gtcttgatcg cctgcgccgc cgccgatgcg cgtcatgagc
     8581 taaaaatatt tagctatctg gcaagcgtat tgtgtcagca tccggcagaa gttattgccg
     8641 ggttaacagg atatgaggca tttatggagt tacttcacaa ggggtgaact gtgtcggatg
     8701 cgccgcatcc gacactgcat tttca
//
LOCUS       DAJVQG010000155         8560 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJVQG010000155 DAJVQG010000000
VERSION     DAJVQG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8560
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.155"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(270..1043)
                     /locus_tag="OQM83_003379"
     CDS             complement(270..1043)
                     /locus_tag="OQM83_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCS1521563.1"
                     /translation="MHDQPLLRMSLPSIPSFVLSGLLLLCLPFSSFACATTSHISFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            1229..1489
                     /gene="dinJ"
                     /locus_tag="OQM83_003380"
     CDS             1229..1489
                     /gene="dinJ"
                     /locus_tag="OQM83_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HCS1521564.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            1492..1770
                     /gene="yafQ"
                     /locus_tag="OQM83_003381"
     CDS             1492..1770
                     /gene="yafQ"
                     /locus_tag="OQM83_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HCS1521565.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            1926..2666
                     /locus_tag="OQM83_003382"
     CDS             1926..2666
                     /locus_tag="OQM83_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCS1521566.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(2637..3404)
                     /locus_tag="OQM83_003383"
     CDS             complement(2637..3404)
                     /locus_tag="OQM83_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCS1521567.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="OQM83_003384"
     CDS             complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="OQM83_003384"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCS1521568.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            4428..6872
                     /gene="fadE"
                     /locus_tag="OQM83_003385"
     CDS             4428..6872
                     /gene="fadE"
                     /locus_tag="OQM83_003385"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCS1521569.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(6915..7361)
                     /gene="ivy"
                     /locus_tag="OQM83_003386"
     CDS             complement(6915..7361)
                     /gene="ivy"
                     /locus_tag="OQM83_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCS1521570.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            7542..8312
                     /gene="yafV"
                     /locus_tag="OQM83_003387"
     CDS             7542..8312
                     /gene="yafV"
                     /locus_tag="OQM83_003387"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCS1521571.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            complement(8354..>8560)
                     /locus_tag="OQM83_003388"
     CDS             complement(8354..>8560)
                     /locus_tag="OQM83_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCS1521572.1"
                     /translation="WRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGL
                     RRKMRKAAMDRNYLASVLAESGLS"
BASE COUNT         2063 a         2148 c         2241 g         2108 t
ORIGIN      
        1 tttgttctcc ttaattatgt aaggagtatt tgatcatgca taagcattaa aaaacagttt
       61 caagtaatga ggaatatctc aaattttaaa cataaaatgc gaattattta gtacaaaaag
      121 tgtacttctg atgatctttc cctgggtgat ggaataacag gaaagcggct cgcaaaaacg
      181 cacagatttg taggcatgat aagacgcgtc agcgtcgcat caggcatctg cgcaccaccg
      241 ccggatgcgg cgtaaacgcc ttatccgtcc tacaaaatcg tctcttccgt caatatccgc
      301 cgcgcgccca aaaaatgatc ctgccagaaa ggttcagcta accgacttac ccgaatggtt
      361 tccccggtgc gcggtgactc aataaactga ccgtcgccca gatacacccc catatggtcg
      421 gcaatctcgc ggctgtggat atggaaaaac agcaaatctc cccggcgcag gtcgttattc
      481 gccacaatgg ttgcccgacg gtagtgatac atctcattgg ccgtacgcgg gagcttcgcc
      541 tcgaggatct tgttgtaggc ataaaaaact aacccactac aatcgaagcc ttgatcgggc
      601 cgcgtgccgc cccagacgta aggcttgcca agctgctgct ccagacggtg aatcgccact
      661 tcggtgatgt tatgcaggtg atcgctgctt aaaaagtggt tgttttccgc cagcgcctgc
      721 cagcgtctgt cactcgcctt gagcggcgca ggaaaccatt ctggatgctg ttttatctgc
      781 tcccggttgt gctggcgcag cgcccttctg ctttgggcat tgccttccac aatgtagctg
      841 gcctgttttt tcagatgggt ttggtactgt tttaataaac gcgcacggtt ttgcatccgc
      901 tggcgggcgg cgtagctgaa agagatatgt gatgtggtgg cgcaggcaaa tgaagaaaac
      961 ggcaagcaaa gcaacagtaa tcccgacaac acaaaagagg gaatcgacgg caaagacatt
     1021 ctcaataatg gctggtcatg cattgatgtt gcagtgtatc ctggcccgta cctgaacaaa
     1081 cttaataaac cattattata aaattaataa atatatttca gtttattatg taaaaacatc
     1141 cgtttattaa attactcatg gattgtcgct gttgctcatt tgagctacaa ttcaagctga
     1201 ataaatatac agcacaggag atcccccaat ggctgctaac gcgtttgttc gcgcccgaat
     1261 cgatgaagat ctgaagaatc aggcggcgga cgtactggcc gggatggggc tgaccatctc
     1321 tgacctggtt cgcataaccc tcacaaaggt cgcgcgtgaa aaggcattgc cgtttgattt
     1381 acgcgagcct aatcaattaa ccattcaatc aatcaaaaac agcgaagctg gcgttgatgt
     1441 tcataaggcc aaagacgctg atgatttatt tgataaatta ggaatttaaa tatgattcaa
     1501 agggatattg aatactcggg acaattttca aaggatgtaa aacttgcaca aaagcgtcat
     1561 aaggatatga ataaattgaa atatcttatg acgcttctta tcaataatgc tttaccgctt
     1621 ccagctgttt ataaagacca cccgctgcaa ggttcatgga aaggttatcg cgatgctcat
     1681 gtcgaaccgg actggatcct gatttacaaa cttaccgata aacttttacg atttgagaga
     1741 actggaactc acgcggcgct ctttgggtaa agcacgctgc cgatgaaatt tgtaaaccct
     1801 gcgccgacag gcttgcgtaa aactcttttg gtgataatgt gagccttcca aatccagact
     1861 attccgattt gggatctatt ttgccgtcct ggcgtgtgta accgttttat caaggaataa
     1921 gcagtatgcg taaaatcgca ttaattcttg cgatgttgtt gatcccgtgc gtttcgtttg
     1981 ccggtttgct gggcagcagt agctcgacca cgccagtgag caaagagtat aagcagcagt
     2041 tgatgggatc ccctgtctac atccagatct tcaaggaaga acgtacgctc gatctctacg
     2101 tcaaaatggg cgagcaatat cagctgctcg acagttataa aatctgtaaa tattccggcg
     2161 gcttagggcc aaaacagcgt cagggcgatt tcaaaagccc ggaagggttt tatagcgtcc
     2221 agcgtaatca gttaaaacca gacagccgtt actacaaagc gattaatatt ggtttcccca
     2281 atgcctatga ccgtgcgcat ggttatgaag ggaaatacct gatgatccac ggcgattgtg
     2341 tttccatcgg ctgctacgca atgaccaatc agggtattga tgagatcttc cagttcgtta
     2401 ctggtgcgct ggtgtttggt cagccgagcg tgcaagtgag tatttacccg ttccgcatga
     2461 ccgacgccaa tatgaagcgc cataaatatt ccaactttaa ggacttctgg gagcaactga
     2521 agccgggtta cgactacttt gagcaaaccc gtaagccacc gaccgtttct gtggtcaatg
     2581 gtcgttacgt ggtcagcaag ccgttaagcc acgaagttgt gcagccacaa ctggcatcaa
     2641 actacacgct ccccgaggca aaataagcgc cattcgcctg gcataatctt ttgccaggtt
     2701 tcattgcccg tcagcggctg tgttgcaatc accgtgacca catcattcgg tgtggtctgc
     2761 gagctgaagt cgatttccac atcctgatcc agcagcgttg ccacgccaaa cggcgcgcgg
     2821 cgggtgatcc agtgtaaatt agtcgagcaa tacgccatca catagcgccc gtcagaaagc
     2881 agcatgttga aaacgccctt ctgccgcagt tcatccgcca gtgaggcgat atatttaaac
     2941 actgccgcca tgttgcccgg tgtgcgcggg taacgctgcg ttaatttatg caggagccag
     3001 cagaaggctt tttcgctatc ggtttcaccg accgggcgga agttgccggt ttccagtgat
     3061 ttgtagcccg tcagttgtcc gttatgggca taagtccagt tacgccccca taactcacgg
     3121 gtaaacgggt gagtattttc cagcgccacc tcgccccgat tggcctggcg aatatgagcc
     3181 accaccgaac aggattttat cgggtagtcc tggacaagtt tggcgatggg ggaattaaag
     3241 ctgggttgtg gatctttaaa tgtgcgacag cctttacctt cgtaaaaggt aatgccccag
     3301 ccatctttat gtggcccggt tccaccacca cgctgcacca gaccggtgaa actaaagcag
     3361 atatcggtag ggacgttggc gctcatcccg agcaattcgc acatgggtaa cctccacaac
     3421 agcgggagcg catggccccc gtcgaagatt gatttaagaa ttttcaggcc ggatgcggcg
     3481 taaacgtctt attcggccta cggttcgcgt acagatttgt aggcatgata agacgcatca
     3541 gcgtcgcatc aggcatcagc gcacaagtgc cggatgcggc gtaaacgcct tatccggcct
     3601 acgccagact tacttaacca tctctttttc aattaactgg atcaggatat ggatcacttt
     3661 aatgtgaatc tcctgaatgc ggtcggcata accaaagtgc ggaacgcgaa tttcgatatc
     3721 cgccgtgcca gccattttac cgccgtcttt accggtcagg gtgatcactt tcatcccctt
     3781 ctcacgcgcc gctgcgatcg ctttgatcac gtttgcagag ttaccggagg tggagatccc
     3841 cagcagtaca tcgccttcgc gacctaccgc ttcaacgtag cgggagaaaa tatcattgaa
     3901 accgaaatca ttaccgacgc aggaaatatg actaacgtca gaaatagcaa tcgccgggta
     3961 gcccggacgg ttttcacggt agcgaccggt caactcttcg gcaaagtgca tagcgtcgca
     4021 atgggaaccg ccgttgccgc aggaaagcac tttgccaccg gctttaaagc tgtctgctaa
     4081 caggaccgcc gcgcgctgaa tggcgtgaat attggcgtca tcttttaaaa agttagccag
     4141 cgtttccgcc gcttcgttca gttcgttacg aataagatcc tggtacatga ggatatcctt
     4201 cagcataaat gtaatagaca aaatgcagtg taccggatac cgccaaaagc gagaagtacg
     4261 ggcaggtgct atgaccagga ctttttgacc tgaagtgcgg ataaaaacag caacaatgtg
     4321 agctttgttg taattatatt gtaaacacat tgctaaatgt ttttacatcc actacaacca
     4381 tatcatcaca agtggtcaga cctcctacaa gtaaggggct tttcgttatg atgattttga
     4441 gtattctcgc tacggttgtc ctgctcggcg cgttgttcta tcaccgcgtg agcttattta
     4501 tcagcagtct gattttgctc gcctggacag ccgccctcgg cgttgctggt ctgtggtcag
     4561 cgtgggtact ggtgcctctg gccattatcc tcgtgccatt taactttgcg cctatgcgta
     4621 agtcgatgat ttccgcgccg gtatttcgcg gtttccgtaa ggtgatgccg ccgatgtcgc
     4681 gcactgagaa agaagcgatt gatgcgggca ccacctggtg ggagggcgac ttgttccagg
     4741 gcaagccgga ctggaaaaag ctgcataact atccgcagcc gcgcctgacc gccgaagagc
     4801 aagcgtttct cgacggcccg gtagaagaag cctgccggat ggcgaatgat ttccagatta
     4861 ctcatgagct ggcggatctg ccgccggagt tgtgggcgta ccttaaagag catcgcttct
     4921 tcgcgatgat tatcaaaaaa gagtacggcg gactggagtt ctcagcttat gcccagtctc
     4981 gcgtgctgca aaaactctcc ggcgtgagcg ggatcctggc gattaccgtc ggcgtgccga
     5041 actcattagg cccgggcgag ctgctgcaac attacggcac tgacgagcag aaagatcact
     5101 atctgccgcg tctggcgcgt ggtcaggaga tcccttgctt tgcactgaca agcccggaag
     5161 cgggttccga tgcgggcgcg attccggaca ccgggattgt ctgcatgggc gaatggcagg
     5221 gccagcaggt gctggggatg cgtctgacct ggaacaaacg ctatattacg ctggcaccga
     5281 ttgcgactgt gcttgggctg gcgtttaaac tctccgaccc agaaaagctg ctgggtggcg
     5341 cagaagattt aggtattacc tgtgcgctga tcccgaccac cacgccgggc gtggaaattg
     5401 gtcgtcgcca cttcccgctg aacgtaccgt tccagaacgg accgacgcgc ggtaaagatg
     5461 tcttcgtacc gatcgattac atcatcggtg ggccgaaaat ggccgggcaa ggctggcgga
     5521 tgctggtgga gtgcctctcg gtaggccgcg gcatcaccct gccttccaac tcaaccggcg
     5581 gcgtgaaatc ggtagcgctg gcaacgggcg cgtatgctca cattcgccgt cagttcaaaa
     5641 tctctatcgg taagatggaa gggattgaag agccgctggc gcgtattgcc ggtaatgcct
     5701 acgtgatgga tgctgcggca tcgctgatta cctacggcat tatgctcggc gaaaaacctg
     5761 ccgtgctgtc ggctatcgtt aagtatcact gtacccaccg cgggcagcag tcgattattg
     5821 atgcgatgga tatcactggt ggcaaaggca ttatgctcgg gcaaagcaac ttcctggcgc
     5881 gtgcttacca gggcgcaccg attgccatca ccgttgaagg ggctaacatt ctgacccgca
     5941 gcatgatgat cttcggacaa ggagcgattc gttgccatcc gtacgtgctg gaagagatgg
     6001 aagcggcgaa gaacaatgac gtcaacgcgt tcgataaact gttgttcaaa catatcggtc
     6061 acgtcggtag caacaaagtt cgcagcttct ggctgggcct gacgcgcggt ttaaccagca
     6121 gcacgccaac cggcgatgcc actaaacgct actatcagca cctgaaccgc ctgagcgcca
     6181 acctcgccct gctttctgat gtctcgatgg cagtgctgga cggcagcctg aaacgtcgcg
     6241 agcgcatctc ggcccgtctg ggggatattt taagccagct ctacctcgcc tctgccgtgc
     6301 tgaagcgtta tgacgacgaa ggccgtaatg aagccgacct gccgctggtg cactggggcg
     6361 tacaagatgc gctgtatcag gctgaacagg cgatggatga tttactgcaa aacttcccga
     6421 accgcgtggt tgccgggctg ctgaatgtgg tgatcttccc gaccggacgt cattatctgg
     6481 caccttctga caagctggat cataaagtgg cgaagatttt acaagtgccg aacgccaccc
     6541 gttcccgcat tggtcgcggt cagtacctga cgccgagcga gcataatccg gttggcttgc
     6601 tggaagaggc gctggtggat gtgattgccg ccgacccaat tcatcagcgg atctgtaaag
     6661 agctgggtaa aaacctgccg tttacccgtc tggatgaact ggcgcacaac gcgctggcga
     6721 aggggctgat tgataaagat gaagccgcta ttctggtgaa agctgaagaa agccgtctgc
     6781 gcagtattaa cgttgatgac tttgatccgg aagagctggc gacgaagccg gtaaagttgc
     6841 cggagaaagt gcggaaagtt gaagccgcgt aattgaataa cggagccgaa aggctccgtt
     6901 tctttatccg ctaattattt aaaattaaag ccatccggat ggttttccag gctgccggtc
     6961 aacgccgcga acagcaccgt tttaccatca atcgaaagcg catcgttcac attcagccag
     7021 gtgagtttct cttgcgacgt tttctcatca atagtcgaga acagccccgt catctgatta
     7081 gatttctcgg accacatcac agcgatacgt tgcgagccac agtcatgcgg tttgcacgcg
     7141 ctcatcacct gatacgtatc atctcccaac gttacggttt gtgcgggagt ataagtaccg
     7201 cctttcatca cccaggcagg cagcttatgc ccttgtacca tctgattaaa tgcagctttg
     7261 gtggtttcgc cctttgcaag gctgctaatg gttaaatcat cctgcgccat tgcactggtg
     7321 gcgatgacca gagcggcgac tgtcgttatt gtcttaaaca tcattcctcc cgagcttatc
     7381 ctgcccatgt tattaacctt ctaaagcctg gcgataacac gataatttgc aaatcagata
     7441 tttccaatat cagcatttgt tatcaattta ttctgcgcta aggcaactgc ctgataaggt
     7501 gttaaagtta taaaaccctt cgcataaagg agttgtacac cgtgcctggt ttgaagatta
     7561 cgcttttgca gcaaccactg gtgtggatgg atggccctgc caacctgcgt cattttgatc
     7621 gtcaactgga aggtattacc gggcgcgatg tgatcgttct accgaagatg tttaccagcg
     7681 gctttgccat ggaagcggca gcttcgtcgc tagcacaaga tgacgtagtg gactggatga
     7741 cagctaaggc gcagcagtgc aatgcgctga ttgcaggcag tgttgcatta caaacggagt
     7801 ctggttcggt taaccgcttt ttgctggttg agccgggcgg cacggtacat ttttatgata
     7861 agcgtcatct gttccgcatg gcagatgagc atctacatta taaagcgggc aatgcgcgag
     7921 tgattgtgga atggcgcggc tggcgtattt tgccgttggt gtgctacgac ttacgttttc
     7981 cggtgtggtc gcgcaatctc aacgattatg acctcgccat ctacgttgcc aactggcctg
     8041 ctccgcgctc tctgcactgg caggcattgc tgacggcccg cgcgattgag aatcaggcgt
     8101 atgtggcggg atgcaatcgc gtcggcagcg atggcaacgg ctgccattat cgcggtgaca
     8161 gccgggtgat taatccacaa ggcgagatta tcgctactgc cgacgcgcat caggcaacgc
     8221 gcattgatgc ggagctgtcg atgatggcat tgcgggaata tagagaaaag tttccggcat
     8281 ggcaggatgc ggatgagttt aggttgcggt gagtgggggt tgtgtttaag gctgggggag
     8341 agtcggggta agactacgaa agcccgctct ccgcaaggac tgacgccaga tagtttctgt
     8401 ccatagctgc ttttcgcatc ttacgtctta accctgcctt gaatacctta tcattcgtca
     8461 aaatattaat agcgatgtgc cgtatccctg aaaataattc tgctgcattt cctcttctta
     8521 ttttgcagtc gtcttcattc attaccacgt ccagacgcca
//
LOCUS       DAJVQG010000156         8486 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJVQG010000156 DAJVQG010000000
VERSION     DAJVQG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8486
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.156"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            52..1220
                     /locus_tag="OQM83_003389"
     CDS             join(52..301,301..1220)
                     /locus_tag="OQM83_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCS1521573.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRKLRKKLQRIEMENEIFKKGYRALDVRLPEQFSII
                     GKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGAR
                     SIATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVT
                     EPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGCAMSFSPDSRLTMKALEMAWETR
                     GKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWM
                     PVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVASFC"
     gene            1216..1503
                     /locus_tag="OQM83_003390"
                     /pseudo
     CDS             1216..1503
                     /locus_tag="OQM83_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
     gene            1504..3120
                     /locus_tag="OQM83_003391"
     CDS             1504..3120
                     /locus_tag="OQM83_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCS1521574.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(3176..3328)
                     /locus_tag="OQM83_003392"
                     /pseudo
     CDS             complement(3176..3328)
                     /locus_tag="OQM83_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3351..3728)
                     /locus_tag="OQM83_003393"
     CDS             complement(3351..3728)
                     /locus_tag="OQM83_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521575.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            complement(4138..4509)
                     /locus_tag="OQM83_003394"
     CDS             complement(4138..4509)
                     /locus_tag="OQM83_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521576.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(4570..5067)
                     /locus_tag="OQM83_003395"
     CDS             complement(4570..5067)
                     /locus_tag="OQM83_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521577.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(5136..5660)
                     /gene="sat2"
                     /locus_tag="OQM83_003396"
     CDS             complement(5136..5660)
                     /gene="sat2"
                     /locus_tag="OQM83_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCS1521578.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(5755..6228)
                     /locus_tag="OQM83_003397"
     CDS             complement(5755..6228)
                     /locus_tag="OQM83_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCS1521579.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            6560..7096
                     /locus_tag="OQM83_003398"
     CDS             6560..7096
                     /locus_tag="OQM83_003398"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCS1521580.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            7211..7537
                     /locus_tag="OQM83_003399"
     CDS             7211..7537
                     /locus_tag="OQM83_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1521581.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(7725..7964)
                     /locus_tag="OQM83_003400"
     CDS             complement(7725..7964)
                     /locus_tag="OQM83_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCS1521582.1"
                     /translation="MTPFRYNSDLTSGSLQTREYRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         2344 a         1852 c         2009 g         2281 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa
      241 caaatcgaaa tacgtaagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttgaaaggc agttcgccgt gaccgagcca aatcaggtgt
      721 ggtgcggtga tgtgacctat atctggacgg gtaagcgctg ggcgtacctc gccgttgttc
      781 tcgacctgtt cgcaagaaaa ccagtgggct gtgccatgtc gttctcgccg gacagcaggc
      841 tcaccatgaa agcgctggaa atggcatggg aaacccgtgg taagcccggc ggggtgatgt
      901 tccacagcga tcagggcagt cattatacga gcaggcagtt ccggcagtta ttgtggcgat
      961 accagatcag acagagtatg agccggcgcg gaaactgctg ggataacagc ccaatggaac
     1021 gcttcttcag gagtctgaag aacgaatgga tgccggtggt gggttacgta agcttcagcg
     1081 aggcagctca cgccataacg gactatatcg ttggatatta cagcgcacta agaccgcacg
     1141 aatataacgg tgggttaccc ccaaacgaat cggaaaatcg atactggaaa aactctaact
     1201 cggtggccag tttttgttga ccacttcaga gcgacatcga gctggctgtt aaagcaaact
     1261 cctaacggaa cctctcttgc aaaaaatcta cagaaactgc ctttggtagc gctttgctta
     1321 aagcgttact cagagagtgt ggaagattat caaattagac ggattagcca agcttttatt
     1381 aagcttaaac aggaagatgt tgagcttagg cgctggcgat tattaagaag tgcaacgtta
     1441 tctaaagagc ggataactga ggaagcacaa agattcttgg aaatggttta tggggaagag
     1501 tgagtggtta ggctagctac atttaatgac aatgtgcagg ttgtacatat tggtcattta
     1561 ttccgtaact cgggtcataa ggagtggcgt atttttgttt ggtttaatcc aatgcaagaa
     1621 cggaaatgga ctcgatttac tcatttgcct ttattaagtc gagctaaggt ggttaacagt
     1681 acaacaaagc aaataaataa ggcggatcgt gtgattgagt ttgaagcatc ggatcttcaa
     1741 cgagccaaaa taatcgattt tcctaatctc tcgtcctttg cttccgtacg caacaaggat
     1801 ggagcgcaga gttcatttat ttacgaagct gaaacaccat atagcaagac tcgttatcac
     1861 atcccacagt tagagctagc tcggtcatta tttttaatta actcctattt ctgtcgaagc
     1921 tgtttgagca gtaccgcttt acagcaagag ttcgacgttc agtatgaggt tgagcgagat
     1981 catttagaga taaggatctt acccagttca tcgtttccta aaggggcgtt agagcagtcg
     2041 gccgtagtgc agcttttggt ttggttgttt tcggatcaag atgttatgga ttcgtatgaa
     2101 agtattttta ggcactatca acaaaataga gaaattaaga acggcgttga aagctggtgc
     2161 tttagctttg accctccgcc catgcagggt tggaaattac atgtaaaagg acgttcttct
     2221 aacgaggata aggattattt agttgaggaa atagtaggtt tagaaatcaa cgctatgctt
     2281 cctagcacaa cagctattag ccatgcctct tttcaggaaa aggaggcagg tgatggtagt
     2341 acgcagcaca tagcggtttc aacagagtca gttgttgatg atgagcatct acagttggac
     2401 gatgaggaaa cagccaatat agacacagac acacgagtca tagaggctga gccgacatgg
     2461 ataagtttta gtagacctag tcgaattgaa aaatctcgca gggcaagaaa aagtagccaa
     2521 actattttag aaaaagaaga agcaacaaca agtgaaaata gtaatttggt tagtactgat
     2581 gagccacact taggtggtgt cctagcagcg gcagatgtgg gtgggaagca ggatgcaacc
     2641 aattacaact ctatttttgc taatcgattt gctgcttttg atgagctact ttcaattcta
     2701 aaaactaaat ttgcatgtcg ggtgcttttt gaagaaacct tggttttgcc aaaagttggg
     2761 cgtagccgat tacatctgtg taaagatggc tcaccaagag tgattaaagc cgttggggtg
     2821 caacgtaatg gcagtgaatt tgtattgcta gaggtggatg catcggatgg ggtgaaaatg
     2881 ctttctacca aagtgttgag tggcgttgat agcgaaacat ggcggaatga ttttgaaaag
     2941 atacggcgtg gagtggtgaa gagctcattg aattggccaa atagtttgtt tgatcaatta
     3001 tatggacaag acgggcatag aggggtgaat catccaaagg ggttggggga gctgcaagta
     3061 tcgagagagg atatggaagg gtgggctgag agagtggtta gagagcaatt tacgcattaa
     3121 aggaatgact gaaagagcct gtaaaccctt ttgtgtaagt gcttttgccg gtcagttaaa
     3181 ggtggccatt taaacggtca ccaaattcga tcataaaacg gttcatggcc ggcttccagt
     3241 tgcggatcgg catcgtccat ttcttggtcg ccgcctggat agccaggtac accaccttca
     3301 tcgctgattc gtccgtaggg aacaccttga gttcatatta atggaatttt ctacaaatag
     3361 cctccgtggt tttgaggggg gattacagac gatccatagt agtaatccaa tgagttcttg
     3421 agcgcggcga catgtttgga cgccttggca aaaattagag cctgcttgaa gtgcaggcga
     3481 gcacgtgctt ggacgattga tccattcgcg gtcaaaaact caatcttgga tgacagcgtg
     3541 tcaggaaatc caatatcgaa gtcccgcccc gtgtcaaagt agggcatttc atgatcaaag
     3601 gacgaagctt cccaggccac ttccctaata ttccagccgc tagaaacaag atttcgtacg
     3661 acttcatcaa cttttttgct ttccaacttg tcacaaagtg cataggcaag agctttggcg
     3721 gcagacacgt gcgtttcctc ttaaacatag gtcgaacgga tacccgaggc aaagcggagc
     3781 attagtccca aagcatctcc ggtagatccg gaggctccat actgaaggca tggcatcttg
     3841 ggcattgata tttgaaactt ctctctagat agcagggatt tcttggcctt gcctcatgct
     3901 gatagcttgt aaatatcaca cgatccttag ccccacaaag tgagcatact ccgtagcttt
     3961 catttataga aatcgtcgtt agattgtcgc agcatgtgca cgttccgact atttgctcag
     4021 gaagcatcgc cataccttgg cctaggtaaa gaagatcaga ctcgtagcca catcggtggc
     4081 aactgagctt gtacgcaatg cccatctggt tgtctcacct gaatcgtaac gtcagagcta
     4141 ttcatccatc cattcggcat caatgtatgc cactaagccg accagctgac cgtttggact
     4201 gaagtatgtt gcctgcaccc cacttgacat ctcatcaata ccaccgcact gcgctaggcg
     4261 ccggtcccac ttgcccacag aagcgtcagg atcggctgag ttccccaaga cccttgtgag
     4321 ccatgcctcc tttaactcaa cctctcgaat gattgcgtcg gccagctcct ccgcgttatc
     4381 tagttcgaca tagtctggaa ttcggacgta cgttctgaac acagctggat acttacttgg
     4441 gcgattgtca tacatgacat caacaatgta cccgtttgtg taaccgtctc ttggaggttc
     4501 gtatgacact agtggttccc ctcagcttgc gactagatgt tgaggcctaa cattttatta
     4561 gagagcaggc tagttgctta gatacatgat cttcaggccg ttatctgtca gggcaagcga
     4621 aaattggcca tttatgacga ccaatgcccc gcagaagctc ccatctttgc cgccatagac
     4681 gccgcgcccc ccttttgggg tgtagaacat ccttttgcca gatgtggaaa agaagttcgt
     4741 tgtcccattg ttggcaatga cgtagtagcc ggcgaaagtg cgagacccat ttgcgctata
     4801 tataagccta cgatttccgt tgcgactatt gtcgtaattg gatgaactat tatcgtagtt
     4861 gctctcagag ttgtcgtaat ttgatggact attgtcgtaa ttgcttatgg agttgtcgta
     4921 gttgcttgga gaaatgtcgt agttggatgg ggagtagtca tagggaagac gagcttcatc
     4981 cactaaaaca attggcaggt cagcaagtgc ctgccccgat gccatcgcaa gtacgaggct
     5041 tagaaccacc ttcaacagat cgcgcatagt cttccccagc tctctaactc ctgaattaag
     5101 ccgcgccgcg aagcggtgtc ggcttgaatg aattgttagg cgtcatcctg tgctcccgag
     5161 aaccagtacc agtacatcgc tgtttcgttc gagacttgag gtctagtttt atacgtgaac
     5221 aggtcaatgc cgccgagagt aaagccacat tttgcgtaca aattgcaggc aggtacattg
     5281 ttcgtttgtg tctctaatcg tatgccaagg agctgtctgc ttagtgccca ctttttcgca
     5341 aattcgatga gactgtgcgc gactcctttg cctcggtgcg tgtgcgacac aacaatgtgt
     5401 tcgatagagg ctagatcgtt ccatgttgag ttgagttcaa tcttcccgac aagctcttgg
     5461 tcgatgaatg cgccatagca agcagagtct tcatcagagt catcatccga gatgtaatcc
     5521 ttccggtagg ggctcacact tctggtagat agttcaaagc cttggtcgga taggtgcaca
     5581 tcgaacactt cacgaacaat gaaatggttc tcagcatcca atgtttccgc cacctgctca
     5641 gggatcaccg aaatcttcat atgacgccta acgcctggca cagcggatcg caaacctggc
     5701 gcggcttttg gtacaaaagg cgtgacaggt ttgcgaatcc gttgctgcca cttgttaacc
     5761 cttttgccag atttggtaac tataatttat gttagaggcg aagtcttggg taaaaactgg
     5821 cctaaaattg ctggggattt caggaaagta aacatcacct tccggctcga tgtctattgt
     5881 agatatatgt agtgtatcta cttgatcgat caggcttttg tatatctccc caccacctga
     5941 aacaatgaca tgatccgtta ttttctttag gttggttaaa gcatctttaa ttgatggaaa
     6001 gatcaatacg ttctcattgt cagatgtaaa acttgaacgt gttacgaccg catactttcg
     6061 gttgggtaat gctcccattg attcaaaagt cttgcgtcca accaacagcc attggttata
     6121 ggtaatagct ttaaacagga gctgttcacc tttggcactc catggaatat cagggccatt
     6181 cccgataact ccattcttcg atatagctac cattagtgat agtttcacaa ttcttcctca
     6241 gaggttaaca ttttattagt aagcatgctc gttttgtcac ccgctgatgc ttaccgttaa
     6301 ttaatataat cacttgtatt aaatagactt tctttaaaaa tacaagacac tctgttatta
     6361 caaatcgtgc atgccgtcta tcctgcttgc acgatgcaca ttatctcacc caaagaactc
     6421 ataacatttt aatttattta gcattaaatt aaaactctac cggtaaacaa gcatctctag
     6481 gcgtaaaaaa caggcacttt tttaaatccc agctagaata ggctgtatag gcagacagtt
     6541 gcaagacaag gagcgtttta tgtctaacag tccattttta aattctatac gcacggatat
     6601 gcgacaaaaa ggttatgcgc tgaaaactga aaaaacttac ctgcactgga ttaagcgttt
     6661 tattctgttt cacaaaaaac gtcatcctca gaccatgggc agtgaagagg tcaggctgtt
     6721 tttatccagc ttagcaaaca gcagacatgt agccataaac acgcagaaaa tcgctttaaa
     6781 tgccctagct tttttgtaca acaggttttt acaacagccg ttgggcgata ttgattatat
     6841 ccctgcaagc aagcctagac ggctaccctc tgttatctct gcaaatgaag tgcaacgcat
     6901 tttgcaggtt atggatactc gcaaccaagt tatttttacg ctgctgtatg gtgcaggttt
     6961 gcgcattaat gaatgcttgc gtttgcgggt taaagatttt gattttgata atggctgcat
     7021 cactgtgcat gacggtaagg gtgggaaaag cagaaacagc ctactgccca cgcgcctaat
     7081 cccagcaata aaataactca ttgagcaagc gcggcttatt cagcaagacg acaacttaca
     7141 aggcgtaggg ccatcgctgc cttttgcttt agatcacaaa tacccttctg cttatcgaca
     7201 agcggcgtgg atgtttgtct ttccctccag cacgctctgc aaccacccgt ataacggcaa
     7261 attatgccgc catcatctgc atgactccgt tgcgcgaaag gcattgaagg cagccgtaca
     7321 aaaagcaggc atcgttagca agcgtgtcac ttgtcataca tttcgtcact cgtttgctac
     7381 gcatctatta caagcggggc gtgatattcg cactgtgcaa gaactcttag ggcataacga
     7441 tgttaagacc acgcaaatct atacgcatgt gttgggtcag cattttgccg gcaccaccag
     7501 tcctgcggat ggactgatgc tacttatcaa tcagtaaaag tagataaata tctttatgta
     7561 gccctgagta gcccctgata ctttttatgg tgaaaaagat aaacaatcat agaggggata
     7621 aatatgagcc agttctacca ttgttcagat aagtttaagc aagatactgt caatcacagt
     7681 gaccattcat ggtcattaca cttcctccag ttgccagaca atgcctaaca taaaaacgca
     7741 aaagcccaaa agtcagagct taggtgttct aggcgttttc taagcgctga agaaatacgt
     7801 cttaccgtag attgcgtgcg tttctgaagc acattttcct tatacatggc tttatcccag
     7861 gcagcttcat caagctcttg gagtaacaag ccagtaataa tgcgatactc tcgggtttgt
     7921 agcgagccgc tggtgagatc ggagttatag cggaaagggg tcatttgtaa ttatttgtgg
     7981 gtggaggata gttaacttat tattgtagtt ggaagaggct gctttccaaa cattagcacc
     8041 tcgtaatgct tgttactgta tattaattta tgctcttagc gcatgttaat gggctattac
     8101 aatgcataat gtccacatca agataactgt ggcttaacac ctgtacaagc gaaaaaaatc
     8161 aatcacctgt ccggaaatac gggatcactg taaagtgttg gtgaggcagt ctaggagggg
     8221 gatttaattt gggatactga tgaaaggatt atgatcagtt ggatatagga tcgtactaag
     8281 ctgagcggca ggttagctag attagttaac cagataagtg aaatctagtt ccaaactatt
     8341 ttgtcatttt taattttcgt attagcttac gacgctacac ccagttccca tctattttgt
     8401 cactcttccc taaataatcc ttaaaaactc catttccacc cctcccagtt cccaactatt
     8461 ttgtccgccc acagggctgc cgaccg
//
LOCUS       DAJVQG010000157         8326 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJVQG010000157 DAJVQG010000000
VERSION     DAJVQG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.157"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(334..1170)
                     /locus_tag="OQM83_003401"
     CDS             complement(334..1170)
                     /locus_tag="OQM83_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCS1521583.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(1170..1973)
                     /locus_tag="OQM83_003402"
                     /note="aph(3'')-Ib"
     CDS             complement(1170..1973)
                     /locus_tag="OQM83_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCS1521584.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCQFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(2034..2849)
                     /gene="sul2"
                     /locus_tag="OQM83_003403"
     CDS             complement(2034..2849)
                     /gene="sul2"
                     /locus_tag="OQM83_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS1521585.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            4038..4187
                     /locus_tag="OQM83_003404"
     CDS             4038..4187
                     /locus_tag="OQM83_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521586.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(4954..5394)
                     /locus_tag="OQM83_003405"
     CDS             complement(4954..5394)
                     /locus_tag="OQM83_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCS1521587.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            5612..6001
                     /locus_tag="OQM83_003406"
     CDS             5612..6001
                     /locus_tag="OQM83_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521588.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            6045..6233
                     /locus_tag="OQM83_003407"
                     /pseudo
     CDS             6045..6233
                     /locus_tag="OQM83_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(6200..7399)
                     /gene="tet(A)"
                     /locus_tag="OQM83_003408"
     CDS             complement(6200..7399)
                     /gene="tet(A)"
                     /locus_tag="OQM83_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCS1521589.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            7505..8155
                     /gene="tetR(A)"
                     /locus_tag="OQM83_003409"
     CDS             7505..8155
                     /gene="tetR(A)"
                     /locus_tag="OQM83_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCS1521590.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
BASE COUNT         1889 a         2291 c         2300 g         1846 t
ORIGIN      
        1 gggcgaggtc cagggcggcc gtcttcacgt tctgccttgc gcagatgaga tagatctagc
       61 gtggactcaa ggctctcgcg aatggctcgc gttggaaact ttcattgaca cttgaggggc
      121 accgcaggga aattctcgtc cttgcgagaa ccggctatgt cgtgctgcgc atcgagcctg
      181 cgcccttggc ttgtctcgcc cctctccgcg tcgctacggg gcttccaggg gtcgtttgcg
      241 ggaaggggcg gaatcctacg ctaaggcttt ggccagcgat attctccggt gagattgatg
      301 tgttcccagg ggataggaga agtcgcttga tatctagtat gacgtctgtc gcacctgctt
      361 gatcgcggcc gcgatagcta gatcgcgttg ctcctcttct ccatccgcgt tccaagctgc
      421 ggaaaggcac ccataagcgt acgcctggtc gagcaggcga cgcggatcga cgtccagcgc
      481 acgagagaat gcgtccgcca tctgtgcaat gcgtctagga tcgagacaaa ggtcgtctct
      541 gtcagccgga tcgtagaaca tattggcggc gccaaagccc acttcaccga ccagaccgac
      601 gggatctatc accagccagc cgcgactgga gaacatgatg ttttcatgat gcagatcgcc
      661 atgtagccca cgcagttccg aggcattgct catcatttga tcggctataa tcgccgcgtg
      721 gacgtagtca gtttgacaac ctgcgttttg atcatcgcgc gcccgctgaa acaaagctgc
      781 aaagcgatcc cggatcggga gaagggcaga aggcaggggt tcctcagatg cggcatacag
      841 cttcgccatt agttccgctg caatttcggt cgcctggtag tcgccgtgct cggcaacgat
      901 gtgagagagc attcgctccc cggcatattc gagcaacatc agattgttct cacgaccgag
      961 caaccggact gctcccctcc cattgcgcca taccagatag tcggccccgc gcagttcatc
     1021 agcaatgtct tctataggtt tcaatccctt gacgattgca ggagtcccgt ctggcaatga
     1081 aactttccaa acgaggctgg aaaaggtgtc cgcaatgaga acaggttgcg aaacgtgcca
     1141 atgagcagga aaaacaggcg gcatgaacat caaccccaag tcagagggtc caatcgcaga
     1201 tagaaggcaa ggcgttcgcg gtcgggggct tcgatcccca atacattgaa taggacagcg
     1261 aaggcgcgct ctgcttcatc tggcgctgcc cagttctctt cggcgttagc aatcatgagt
     1321 gccaaatcgg catagcgatc tgctgttccg agccgcccaa ggtcgatcag acccgtgcat
     1381 tgaagagttt tagggtccac catgaagttc ggcatgcagg gatcaccatg gcaaacaacc
     1441 atatcggtgc gctcttggtc gagccgcacc ggtagctctc gttcgacacg agccaaaaga
     1501 tcgagctgcg gcgtactctt gtcctcgtcc ggtaagaagt cgggattgac ggcattgcgg
     1561 gacaccacat caacggcgcg tccgaacatt cgcgacagcc tgcgctcaaa ctgacattga
     1621 tcaaccgata ggctgtgaac agcgccaagt tgctgcccca ttgacggcca cgctttgagc
     1681 aaatccgctc cagacagatc agccgccggt actcccggaa ttgccgttat caccaagcat
     1741 gcaccctcct gttcctcctg ccagttgatg acctcggggc aagccacacc tcgacctttg
     1801 agccaaatga ggcggtcacg ctctccagcg agctcaccgc ggcgggaagc aggtgcgatt
     1861 ttcgcgaagg catgcccgtc accacgtcga aaaacaaaat caccagattc tccgcctctg
     1921 acaggcaacc agtcagaatg cgattcacca aaaaaaatat tagttcgatt caatggaggt
     1981 tccttcagtt ttctgatgaa gcgcgaatat agagaaatat cccgaatgtg cagttaacga
     2041 attcttgcgg tttctttcag cgccgccaat accgccagcc cgtcgcgcaa ggggcgcggc
     2101 tcgtgtgtgc ggatgaagtc agctccacct gcggcggcgg caagctctgc agcgagtgtc
     2161 gcggccccga catcccccgg accacggcct gtgagcgcgc gcagaaagga tttgcgcgaa
     2221 acagacagaa gcaccggcaa atcgaagcgc agccgcaatt catcgaaccg cgccagcacc
     2281 gagagcgagg tttcgggagc agcccccaga aaaaacccca tgccgggatc aaggacaagg
     2341 cggttgcgtt tgataccggc acccgtcagc gccgcgatgc gcgcgtcaaa gaacgccgca
     2401 atgtgatcca tgatgtcgcc agcgggtgcc tcgcgccgat ctgcctgccc gtcttgcacc
     2461 gaatgcataa cgacgagttt ggcagatgat ttcgccaatt gcggatagaa cgcagcgtct
     2521 ggaaaaccgc gaatatcatt gagataggcc acaccacgcg acaaggcata ggcttgcgtc
     2581 gcgggttgat aactgtcgag cgagacggga atgccatctg ccttgagcgc gtccagcacc
     2641 ggcgcgatac gcgcgatttc tgtgtcggac gaaacaggcg cggcgtcggg attgctggat
     2701 gccggaccga ggtcgatcac atctgccccc tcggccatca gcttacgcgc ctgcgcaatg
     2761 gctgcgtctg gcgccagata ccggcctcca tcggagaaac tgtccgaggt tatgttgacg
     2821 atgccgaaaa tgatgagcga tttattcatg ggggcttcta taataataat aatcgagcat
     2881 gagtctcata cggatgctcg ggtcgaaagg gaatccccag gcgagtaacc tgtttgcggt
     2941 gatccgtttc atgatgatgt atctgtagca ttttggaaat aaatatatca tctctcaata
     3001 agtagatata tttatctttt ttgcttcttt ggtgctgcaa gtcaccaata cgagggaaac
     3061 ctgttacgga gcggtttaac cgtaatcaac agtgactggt gtcatgttcg gaagattaat
     3121 tgatagtaat ctacaaagga tcttcttgag atccttttca atgaatcgta atctcttgct
     3181 ctgtaaacga aaaaaccgcc tggggaggcg gtttgttcga aggttaagtc agttggggaa
     3241 ctgcttaacc cggtaacagg gttcacgaga ccgccattac caaaactgtt ctttcagtgt
     3301 agccgcagtc aggcctctac ttcaagacct cttaatctat cttctcgtgc actcctgtta
     3361 ctaatggctg ctgccagtgg cgttttgtcg tgtctttccg ggttggactc aagttgatag
     3421 ttaccggaca aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg
     3481 gagcgaactg ccttcacgga actgagtgtc aggcgtggaa tgagaaaccg cggccataac
     3541 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagctacca
     3601 gggggaaacg cctgggatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt
     3661 cagattttgc gatgcttgtc aggggggcgg agcctatgga aaaacggctt cgccgtggcc
     3721 ttatctcttc tttgctaagt gtcttcctgg tatcttccag gaaatttccg tccccgtccg
     3781 taagccggta ccgctcgccg cagccgaacg accgagcgta gcgagtcagt gagcgaggaa
     3841 gcggaatatc tcctgtatcg catattctgc tgacgcgccg gtgctgcctt ttttctcctg
     3901 ccacatgaag cacttcactg attttcgcaa tcgtggcaac atagtaagcc agtatacact
     3961 ccgctagcgc tacatgactg gttcagggct gcgccccgaa acccgctaac aacctctcag
     4021 ctactggata tcaccccatg ccatctgaat atgatgaaca actggattcg actgatacgc
     4081 agcttcccgg cgaatatgac ctttttgatt taatggcgca ggttactgaa gagaacctgc
     4141 atgcccctgt ggattttggg tcgcctgtcg gccgggaagc gttatagctt tttcgcttgc
     4201 ccgcgtacgt ggtaatactc cggtattacc ttttatttct ttattttcat gtaattacag
     4261 tggattttta atacccgggt attactcgtg aaaacgaaat attccttttt tttcttttaa
     4321 ttcatgcagt tgcgatgaaa atcgcggctg cgttaggtgt atgaccattt tgaggttgaa
     4381 aaataatcaa ccatggcggg tgatttacgc aggttaaggg gagatcgtga caaaaaaagc
     4441 gttcctcact gtatcgttca tgcctgcatg aacgggttat tcttcctgac tacagaatta
     4501 catcgctcct tcagttcata catcactttt acatcactta tacatcactt ttacatcact
     4561 tatacatcac ttttacatca ttccttcagc tcatacgtca ctttcatcct ctgtgccatt
     4621 tgtacatcac ccccttcctg aacgtgctga cagcgggttt tccggctgcg ccggcaacgc
     4681 ttcacggcca cgcaataaat tgcgtccccg ttcaccgttt tcggatggga agaacgccgg
     4741 aaacaggact taccccggct gaatggtgtg aatttccgcg ctatgcactt gtgcgcatac
     4801 tcatgcatgc cgtaaaaaca gagcctgcgc gtttctggcg ggttttcggg tggtttgttg
     4861 cctgttttac cggtttcccg tcagaaacgc cctgagggcc tctcaggcgg tgctgataac
     4921 agatgttatg gtaaatcaga gtcacctgca ctgttactct gaaccggagc gaaacagccg
     4981 cgaccagaat ccccgctttt tttcctgtgc cagcgcgtca gccagacggc taatttcggc
     5041 ctgtagttgt tccctctcct catttcgcag gcggttttct gcttcccgcg ccatcttgtc
     5101 ttcaagcatt agggtaacag tctgctgcag agcgtccagt ttctgctgaa tgacgtctag
     5161 ctgagcattt tccgcctggc taacgacaga tttggagtgt ccctccttgt gcctttttgg
     5221 tgtctcagaa gtgcgcagct cgccataggc ccgaatcagt tcactggtct cgaactcccg
     5281 gcgatcattt tttcctgtgc gatagctgac aaggccttta gccatgtcac gatataattg
     5341 cgagcgcgac tttcctgtta atttcagtgt ctcttgaact gtgtgccatg ccataaaagt
     5401 gacagtgtct cctgtgtgta ccgtattgta acagcagaac atattactgt tttaacacaa
     5461 aaggtctgag gctgcccgtg aggcgtgaaa aatacacaaa aaacctgttg acggtgtggt
     5521 tttgacgggc gtagtatttc gaatactgta tataaacaca ttgttataaa tacagttttt
     5581 gctgttttta aaagtcaaag aggataattt tatgagtaca gagttaaata gcggtactgt
     5641 cggtggtttt ggtcacatgg gaccaaatga cccctacaat gatggtcata gcggctctgg
     5701 aagttcggga ggtagtttta acggtggtgg taatggtggc catgataata gtagtggttc
     5761 atcttatact gcatcctttg gtctcatcac tggccgacac ccgataactg tcccaggtac
     5821 gaagaatatt aattctgtcg atctttctgc tgtcattaat aacattatac atcatgaagg
     5881 tgcacgcaat aacatgtacc tagatactgc gaacaatgta agagtgggag tgacgggcac
     5941 tggctggcaa tgtctagcaa cggcaggcat ttcggctgag ggtaaaagaa ctttccgcta
     6001 agcgatagac tgtatgtaaa cacagtattg caaggacgcg gaacatgcct catgtggcgg
     6061 ccaggacggc cagccgggat cgggatactg gtcgttacca gagccaccga cccgagcaaa
     6121 cccttctcta tcagatcgtt gacgagtatt acccggcatt cgctgcgctt atggcagagc
     6181 agggaaagga attgccgggc tatgtgcaac gggaatttga agaatttctc caaagcccgc
     6241 gacgcagcgc cggcaggcag agcaagtaga gggcagcgcc tgcaatccat gcccacccgt
     6301 tccacgttgt tatagaagcc gcatagatcg ccgtgaagag gaggggtccg acgatcgagg
     6361 tcaggctggt gagcgccgcc agtgagcctt gcagctgccc ctgacgttcc tcatccacct
     6421 gcctggacaa cattgcttgc agcgccggca ttccgatgcc acccgaagca agcaggacca
     6481 tgatcgggaa cgccatccat ccccgtgtcg cgaaggcaag caggatgtag cctgtgccgt
     6541 cggcaatcat tccgagcatg agtgcccgcc tttcgccgag ccgggcggct acagggccgg
     6601 tgatcattgc ctgggcgagt gaatgcagaa tgccaaatgc ggcaagcgaa atgccgatcg
     6661 tggtcgcgtc ccagtgaaag cgatcctcgc cgaaaatgac ccaaagcgcg gccggcacct
     6721 gtccgacaag ttgcatgatg aagaagaccg ccatcagggc ggcgacgacg gtcatgcccc
     6781 gggcccaccg gaacgaagcg agcgggttga gagcctcccg gcgtaacggc cggcgttcgc
     6841 ctttgtgcga ctccggcaaa aggaaacagc ccgtcaggaa attgaggccg ttcaaggctg
     6901 ccgcggcgaa gaacggagcg tggggggaga aaccgcccat cagcccaccg agcacaggtc
     6961 ccgcgaccat cccgaacccg aaacaggcgc tcatgaagcc gaagtgccgc gcgcgctcat
     7021 cgccatcagt gatatcggca atataagcgc cggctaccgc cccagtcgcc ccggtgatgc
     7081 cggccacgat ccgcccgata tagagaaccc aaaggaaagg cgccgtcgcc atgatggcgt
     7141 agtcgacagc agcgccggcc agcgagacga gcaagaccgg ccgccgcccg aaacgatccg
     7201 acagcgcgcc cagcacaggt gcgcaggcaa attgcatcaa cgcatacagc gccagcagaa
     7261 tgccatagtg ggcggtgacg tcgttcgagt gaaccagatc gcgcaggagg cccggcagca
     7321 ccggcataat caggccgatg ccgacagcgt cgagcgcgac agtgctcaga attacgatca
     7381 ggggtctgtt gggtttcacg tctggcctcc ggaccagcct ccgctggtcc gattgaacgc
     7441 gcggattctt tatcactgat aagttggtgg acatattatg tttatcagtg ataaagtgtc
     7501 aagcatgaca aagttgcagc cgaatacagt gatccgtgcc gccctggacc tgttgaacga
     7561 ggtcggcgta gacggtctga cgacacgcaa actggcggaa cggttggggg ttcagcagcc
     7621 ggcgctttac tggcacttca ggaacaagcg ggcgctgctc gacgcactgg ccgaagccat
     7681 gctggcggag aatcatacgc attcggtgcc gagagccgac gacgactggc gctcatttct
     7741 gatcgggaat gcccgcagct tcaggcaggc gctgctcgcc taccgcgatg gcgcgcgcat
     7801 ccatgccggc acgcgaccgg gcgcaccgca gatggaaacg gccgacgcgc agcttcgctt
     7861 cctctgcgag gcgggttttt cggccgggga cgccgtcaat gcgctgatga caatcagcta
     7921 cttcactgtt ggggccgtgc ttgaggagca ggccggcgac agcgatgccg gcgagcgcgg
     7981 cggcaccgtt gaacaggctc cgctctcgcc gctgttgcgg gccgcgatag acgccttcga
     8041 cgaagccggt ccggacgcag cgttcgagca gggactcgcg gtgattgtcg atggattggc
     8101 gaaaaggagg ctcgttgtca ggaacgttga aggaccgaga aagggtgacg attgatcagg
     8161 accgctgccg gagcgcaacc cactcactac agcagagcca tgtagacaac atcccctccc
     8221 cctttccacc gcgtcagacg cccgtagcag cccgctacgg gctttttcat gccctgccct
     8281 agcgtccaag cctcacggcc gcgctcggcc tctctggcgg ccttct
//
LOCUS       DAJVQG010000158         8297 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJVQG010000158 DAJVQG010000000
VERSION     DAJVQG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8297
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.158"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            247..768
                     /gene="fecI"
                     /locus_tag="OQM83_003410"
     CDS             247..768
                     /gene="fecI"
                     /locus_tag="OQM83_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCS1521591.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            765..1718
                     /gene="fecR"
                     /locus_tag="OQM83_003411"
     CDS             765..1718
                     /gene="fecR"
                     /locus_tag="OQM83_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCS1521592.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1805..4129
                     /gene="fecA"
                     /locus_tag="OQM83_003412"
     CDS             1805..4129
                     /gene="fecA"
                     /locus_tag="OQM83_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCS1521593.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMNAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            4174..5076
                     /gene="fecB"
                     /locus_tag="OQM83_003413"
     CDS             4174..5076
                     /gene="fecB"
                     /locus_tag="OQM83_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCS1521594.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            5073..6071
                     /gene="fecC"
                     /locus_tag="OQM83_003414"
     CDS             5073..6071
                     /gene="fecC"
                     /locus_tag="OQM83_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCS1521595.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            6068..7024
                     /gene="fecD"
                     /locus_tag="OQM83_003415"
     CDS             6068..7024
                     /gene="fecD"
                     /locus_tag="OQM83_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCS1521596.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            7025..7792
                     /gene="fecE"
                     /locus_tag="OQM83_003416"
     CDS             7025..7792
                     /gene="fecE"
                     /locus_tag="OQM83_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCS1521597.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
BASE COUNT         1804 a         2379 c         2314 g         1800 t
ORIGIN      
        1 cagcattacc gaaatcccta tcctttaggg cagtgaggat gtcaataata gcagaccaca
       61 tcatgagtca ttctgaccga cacatgccag aagcagaggc tccatcccat tttataccta
      121 ccttataaca cttagaaaaa caacatgtta aaaatgtcta ttggaaacaa ttttatttcc
      181 aattgtaatg ataactattc tcatattaat atggctacgt gataattaac ttttgatgca
      241 ctccgcatgt ctgaccgcgc cactaccaca gcttccttaa cattcgagtc gctttatggc
      301 acacatcacg gctggttgaa aagctggctg acgcgcaaac tccagtctgc ttttgatgca
      361 gatgacattg cccaggacac ttttttgcgg gtaatggtca gcgaaacgct ctcgacgatc
      421 cgcgatcctc gctccttcct ctgcactatc gccaaacgcg tgatggtgga cctgtttcgc
      481 cgaaacgcgc tggaaaaagc gtatctggag atgctggcgc ttatgccgga ggggggagcg
      541 ccttcacctg aggaacgcga aagccaactc gagaccctac aactcctcga cagcatgctg
      601 gacgggctaa acggcaaaac acgtgaagcg tttctgcttt cgcaactgga tggtctgaca
      661 tacagcgaga ttgcgcacaa actcggtgtt tccatcagct ccgtgaaaaa atacgtggcg
      721 aaagccgtcg agcactgcct gctgttccgt ctggagtatg ggttatgaat cctttgttaa
      781 ccgattcccg ccgtcaggcg ctgcgttcag cttcccactg gtatgccgtg ctaagcggcg
      841 agcgcgtcag cccacaacag gaagcgcgct ggcaacagtg gtatgaacag gatcaggata
      901 accagtgggc ctggcagcag gttgaaaacc tgcgcaacca gcttggcggt gtgcctggcg
      961 acgttgccag ccgggcgttg cacgataccc gcctcacccg ccgtcacgtg atgaaaggat
     1021 tactgctgtt gctcggcgct ggcggaggct ggcagctctg gcagtcggaa accggcgaag
     1081 gtctgcgggc agattaccgc accgccaaag gcacagtcag ccgtcagcaa ctggaagatg
     1141 gctccctgct cacgctgaat acccaaagcg cggcggatat gcgttttgat gcgcatcagc
     1201 gcaccgtccg gctctggtac ggtgaaatcg ccattactac cgcgaaagat gccctgcaac
     1261 gccccttccg tgtcctgacc cgtcagggcc agctcactgc tttagggaca gaatttaccg
     1321 tccgccagca ggataatttc acgcagcttg acgtgcagca gcacgccgtg gaagtgcttc
     1381 tcgccagtgc ccccgcgcaa aaacgcatcg tgaacgctgg tgaaagcctg cagttcagcg
     1441 cctctgagtt tggcgcagtg aaaccgctgg atgacgagag tacaagctgg acgaaggaca
     1501 tcctgagctt cagcgataaa ccgctgggtg aggtgatagc cacgctaacc cgttaccgca
     1561 acggcgtgct gcgctgcgat cccgccgttg ccgggctgcg cctgagcggg acgttcccgc
     1621 tgaaaaatac cgatgcgatc ctgaacgtta tcgcgcaaac gcttcccgtt aaaattcagt
     1681 ctattacgcg gtactggata aacatttcac cactgtaagg aaaataattc ttatttcgat
     1741 tgtccttttt acccttctcg ttcgactcat agctgaacac aacaaaaatg atgatgggga
     1801 aggtatgacg ccgttacgcg tttttcgtaa aacaacacct ttggttaacg ccattcgcct
     1861 gagcctgctg ccgctggccg gtctctcgtt ttccgctttt gctgcacagg ttaatatcgc
     1921 accgggatcg ctcgataaag cgctcaatca gtatgccgca cacagcggat ttaccctctc
     1981 ggttgacgcc agcctgacgc gcggcaagca gagcaacggc ctgcacggcg attacgacgt
     2041 cgagagcggc ctgcaacaac tgctggacgg cagcggactg caggtaaaac cgctgggaaa
     2101 taacagctgg acgctggagc ccgcgcccgc accaaaagaa gatgccctga ccgtggtcgg
     2161 cgactggctg ggtgatgcgc gtgaaaacga cgtatttgaa catgctggcg cgcgtgacgt
     2221 gatccgccgt gaggatttcg ccaaaaccgg cgcaaccacc atgcgtgagg tacttaaccg
     2281 catccctggc gtcagcgcgc cggaaaacaa cggcaccggc agccacgacc tggcgatgaa
     2341 ctttggcatc cggggcctga acccgcgcct cgccagccgc tcgaccgtcc tgatggacgg
     2401 catccccgtc cccttcgccc cttacggtca gccgcagctt tcactggctc ccgtttcgct
     2461 cggcaacatg aatgccattg acgtggtacg cggtggtggt gcggtgcgtt acggaccgca
     2521 gagcgtgggc ggcgtggtga actttgttac ccgtgccatt ccgcaggact ttggtatcga
     2581 ggcgggcgtg gaaggtcagc tcagcccaac ctcttcacaa aacaacccga aagagacgca
     2641 caacctgatg gtgggcggca cagcggacaa cggttttggc accgcgctgc tctactccgg
     2701 cacgcgcggc agtgactggc gcgagcacag cgccacccgc atcgacgacc tgatgctgaa
     2761 aagcaaatat gcgccggatg aggtgcacac cttcaacagc ctgctgcaat attacgacgg
     2821 tgaagccgac atgcccggtg gcctgtctcg cgcggattac gacgccgatc gctggcaatc
     2881 cacccgcccg tatgaccgct tctggggtcg tcgcaagctg gcgagcctgg gctaccagtt
     2941 ccagccagac agccagcata aattcaacat tcaggggttc tacacccaaa ccctgcgcag
     3001 cggctacctg gagcaaggca aacgcatcac cctctcgccg cgtaactact gggtgcgcgg
     3061 tattgagcca cgctacagcc agatctttat gatcggccct tccgcgcacg aagtgggcgt
     3121 gggctatcgc tatgtgaatg aatcaacgca tgaaatgcgt tactacaccg ccaccagcag
     3181 cgggcagttg ccgtccggct caagccctta cgaccgcgat acgcgttccg gcaccgaggc
     3241 gcacgcctgg tatctggatg acaaaatcga catcggcaac tggaccatca cgccgggtat
     3301 gcgtttcgaa catatcgagt cataccagaa caacgccatc acaggcacgc acgaagaagt
     3361 gagctataac gcaccgcttc cggcgttgaa cgtgctctat cacctgactg acagctggaa
     3421 tctttatgca aacactgaag gctcgttcgg caccgtacag tacagccaga ttggcaaggc
     3481 tgtgcaaagc ggcaatgtgg aaccggaaaa agcgcgaacc tgggaactcg gtacccgcta
     3541 cgacgacggc gcgctgacgg cggaaatggg gctgttcctg attaacttta acaatcagta
     3601 cgactccaac cagaccaacg acaccgtcac tgcacgtggc aaaacgcgcc ataccgggct
     3661 ggaaacgcag gcacgttacg atctgggtac gctaacgcca acgcttgata acgtttccat
     3721 ctacgccagc tatgcgtatg tgaacgcgga aatccgcgag aaaggcgaca cctacggcaa
     3781 tctggtacca ttctccccga aacataaagg cacgctgggc gtggactaca agccaggaaa
     3841 ctggatgttc aatctgaaca gcgatttcca gtccagccag tttgcggata acgccaatac
     3901 ggtgaaagag agcgccgacg gcagtaccgg ccgcattccc ggcttcatgc tctggggcgc
     3961 acgcgtggtg tatgactttg gcccgcagat ggcagatctg aacctggcat tcggtgtgaa
     4021 aaacatcttc gaccaggact acttcatccg ctcttatgac gacaacaaca aaggcatcta
     4081 tgcaggccag ccgcgcacgc tgtatatgca gggggcgttg aagttctgat gtgttaacgc
     4141 ccggcttgcc gggcttttag ctggaatgtg attatgttgg catttatccg ttttcttttt
     4201 gcaggcctgc tgctggtgat cagccacgcc tttgccgcca cggttcagga cgaacacggc
     4261 acgtttacac tcgaaaaaac gccacaacgg attgtggtgc tggaactctc gttcgccgat
     4321 gcgctggccg ccgtggacgt cagcccgatc ggtattgccg acgataacga tgcaaaacgc
     4381 atcctgcccg aagtgcgtgc gcacctgaaa ccgtggcagt ccgtcggaac gcgcgcgcag
     4441 ccgagcctgg aagccattgc cgctctgaaa ccagacctga tcattgccga cagcagtcgc
     4501 catgcggggg tttacatcgc cttgcagcaa atcgcgtcgg tactgctgct taagtcccgc
     4561 aacgaaacct acgctgaaaa tttgcaatct gcggctatca tcggcgaaat ggtgggtaaa
     4621 aagcgagaga tgcaggcacg tctggaacaa cataaagaga ggatggcgca gtgggccagc
     4681 cagcttccca aagggacacg cgtggccttt ggcacatcac gcgaacagca attcaacctg
     4741 catactcagg agacctggac cggcagcgtg ctggcctctc tggggctgaa cgttcccgct
     4801 gcgatggcgg gtgcgtccat gccgtccatc ggcctggagc aactgctggc ggtcaatcct
     4861 gcctggctgc tggttgccca ctatcgcgaa gagagcattg ttaaacgctg gcaacaagat
     4921 ccgctctggc agatgttaac cgccgcgcag aagcagcagg ttgcttcggt cgacagtaac
     4981 acctgggcgc ggatgcgcgg tatttttgct gcagagcgta ttgccgctga cacggtaaaa
     5041 atcttccacc atcagccgct taccgttgtg aaatgaccgc gataaaacac ccggtgctgc
     5101 tgtgggggct tcccgttgca gcacttatta ttattttctg gctgagtctg ttttgctact
     5161 cggccattcc tgtttccgga gcagatgcaa cccgcgccct gctgcctgga cacacgccaa
     5221 cgctaccaga agcgctggtg caaaaccttc gtttgccacg aagcctggtc gccgttctga
     5281 tcggcgcaag cctggcgctc gcgggcacgc tgctgcaaac cctgacccac aacccaatgg
     5341 cctctccttc actgctcggc attaacagcg gcgcggcgct ggctatggcg cttaccagcg
     5401 cgctgagtcc gacgccgatt gcaggctatt ctctgtcgtt catcgcggca tgcgggggcg
     5461 gcgtgagctg gctgctggtc atgaccgcag gaggcggatt tcgtcatacc catgacagaa
     5521 acaaactgat cctcgcgggt atcgcgctgt cggccttttg tatgggcctt acccgcatca
     5581 ccctgctgct ggccgaagat catgcttacg gcatctttta ctggctggct ggcggagtgt
     5641 cccacgcccg ctggcaggat gtctggcagc tcttgccggt ggtggtcact gcagtccctg
     5701 tcgtgttgct gctggcgaat caactgaacc tgctcaacct cagcgacagc accgcccata
     5761 cgctgggagt gaacctgacg aggctacgtt tggtcatcaa tatgttagtg ctgcttctgg
     5821 ttggtgcgtg cgtcagcgtg gcaggtccgg tggcgtttat cggtctgctg gtgccacatc
     5881 tggcgcgctt ctgggcaggc ttcgatcagc gcaacgtact gccagtgagc atgctgctgg
     5941 gggccacgct gatgctgctg gcagatgtac tcgcacgcgc gctggccttc cccggagatc
     6001 tgcccgcagg cgcagtgctg gcgctgattg gcagcccttg ctttgtctgg cttgtgagga
     6061 ggcgaggatg aaaattgcgc tggttatttt catcaccctt gccctggcgg gctgtgcgct
     6121 gttatcactc catatgggag tgatccccgt gccgtggcgc gcgctgctga ccgactggca
     6181 ggccggacgc gagcattatt atgtattgat ggagtaccga ctgccgcgct tgctgctggc
     6241 actgtttgtc ggtgcagccc tcgccgtggc gggcgtgctg atacagggga ttgtgcgcaa
     6301 ccctctggca tcaccggata ttctcggcgt taaccatgcc gccagcctgg cctctgtggg
     6361 ggctctactt cttatgccgt cactgcccgt gatggtgctg ccgctgctgg cctttgcggg
     6421 cggcatggcg gggttgatat tgctgaagat gctggcaaag acccaccagc cgatgaagct
     6481 ggcactcacc ggcgtggcgc tttctgcatg ctgggccagc ctgacggatt atctgatgct
     6541 ctcgcgcccg caggatgtga acaacgccct gctgtggctg accggcagct tatggggccg
     6601 tgactggagc tttgtgaaga ttgccatccc gctgatgatt ttatttctgc cgctgagcct
     6661 gagtttttgc cgcgatctcg acctccttgc actcggcgat gcgcgcgcca ccacgctcgg
     6721 tgtgtcggtg ccctataccc gattctgggc tttgttacta gctgtcgcca tgacatctac
     6781 cggcgtggcc gcctgcggcc cgattagctt tattggtctc gtggtgccgc atatgatgcg
     6841 tagcatcacc ggtggacgtc accgcagact gctgcctgtt tcggccctga caggtgcgtt
     6901 gctgttggtg gttgccgatc tgctggcgag aattattcat cccccactgg agctcccggt
     6961 tggcgtgctg accgccatta tcggtgcgcc gtggtttgtc tggttgcttg tgagaatgcg
     7021 ataaatgact ttacgaactg aaaatctgac ggtcaattac ggaacagaca aggtacttaa
     7081 cgacgtttca ctctcactgc caacggggaa gatcaccgcc ctgatcggtc ctaacggttg
     7141 cgggaaatcg acgctgttaa actgtttttc gcggctttta atgccgcagt ctggcaccgt
     7201 atttctcggc gataatccca taaatatgct ctcatcgcgc cagttggccc gcaggctttc
     7261 gctgctgcct cagcaccatt taacgccaga ggggatcaca gtccaggagc tggtttcgta
     7321 tggtcgtaat ccctggctgt cactctgggg gcgtctctcc gctgaagaca atgcacgagt
     7381 taatgtcgcc atgaaccaga cccggatcaa tcatcttgcc gttcgtcggt taaccgagct
     7441 ttccggcggt cagcgccagc gcgcatttct ggcaatggtc ctggcccaga atacgcccgt
     7501 tgtattactt gatgagccaa ccacctatct tgatatcaat caccaggtgg acctgatgcg
     7561 gttgatgggc gaactccgga ctcaggggaa aacggtggtc gctgtgctgc acgaccttaa
     7621 tcaggctagc cggtactgcg atcaactggt ggtaatggca aacggacatg ttatggcgca
     7681 aggcacacca gaagaggtga tgaccccagg attgctgaga acagtattca gcgtggaagc
     7741 ggaaatacac cccgagccgg tatctggcag gccgatgtgc ctaatgaggt agattgcaca
     7801 ggccgtaaga accaaaccac gactgaatga aactggactg gcgccagcaa gcctgttcag
     7861 actggggctg aacttttccg gactctgaaa gattaccaat actcatcgtc catccgcttg
     7921 ctttaggctg acaggttcat aatcaacgca aaccagagct gtacaggctt gggcgcggct
     7981 ttcaaaccag tcgtgatcac ggcaatcaat tttgaactct gcttaacgga catttctgta
     8041 taacccttac ggcaacgaaa aacgcgaagt taaaatttta gaaacccaaa aacgtgacat
     8101 gactaagttt agatttcagg ggggggagat caaaaaattt cgctctgtgc cagagcggac
     8161 attcacggag ctggttcatt accaatgagg ttgggctttt gaggataaat caatgatcag
     8221 acgccaacgt aaatcaaaag caaccctgga acggaattgc taatccagtt tctgaccatc
     8281 gatttttcta aaaagtg
//
LOCUS       DAJVQG010000159         8157 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJVQG010000159 DAJVQG010000000
VERSION     DAJVQG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.159"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..449)
                     /locus_tag="OQM83_003417"
     CDS             complement(<1..449)
                     /locus_tag="OQM83_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCS1521598.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFN"
     gene            complement(566..1612)
                     /gene="ansB"
                     /locus_tag="OQM83_003418"
     CDS             complement(566..1612)
                     /gene="ansB"
                     /locus_tag="OQM83_003418"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCS1521599.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(1788..2507)
                     /locus_tag="OQM83_003419"
     CDS             complement(1788..2507)
                     /locus_tag="OQM83_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCS1521600.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(2691..3017)
                     /locus_tag="OQM83_003420"
     CDS             complement(2691..3017)
                     /locus_tag="OQM83_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCS1521601.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(3017..3736)
                     /gene="trmB"
                     /locus_tag="OQM83_003421"
     CDS             complement(3017..3736)
                     /gene="trmB"
                     /locus_tag="OQM83_003421"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCS1521602.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            3897..4949
                     /gene="mutY"
                     /locus_tag="OQM83_003422"
     CDS             3897..4949
                     /gene="mutY"
                     /locus_tag="OQM83_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCS1521603.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKTKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4977..5252
                     /locus_tag="OQM83_003423"
     CDS             4977..5252
                     /locus_tag="OQM83_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCS1521604.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            5317..6396
                     /gene="mltC"
                     /locus_tag="OQM83_003424"
     CDS             5317..6396
                     /gene="mltC"
                     /locus_tag="OQM83_003424"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCS1521605.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLDGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            6598..7854
                     /gene="nupG"
                     /locus_tag="OQM83_003425"
     CDS             6598..7854
                     /gene="nupG"
                     /locus_tag="OQM83_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCS1521606.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7903..>8157)
                     /locus_tag="OQM83_003426"
     CDS             complement(7903..>8157)
                     /locus_tag="OQM83_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCS1521607.1"
                     /translation="IRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQR
                     YFLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
BASE COUNT         1923 a         2159 c         1987 g         2088 t
ORIGIN      
        1 taattgaaat cttcgctatt cacgatcaga tatttattat tcgttttgat atcgtcgacc
       61 acccgcccac gatcgctggc tgccgcctga aaagagtaaa agttcaccga ccagccagac
      121 gcgggcgttc ctttactggt taacatcgcc gagaatttta ccggtttatc tttcgtccag
      181 gtctgttcga gcagttcgta atctgccaac tgaccgaccc aggtatagag atcgtcactg
      241 gaagagacat cgccttccgc tgaccaggtg gcctgattac ccttagcatc aagatttttt
      301 aaggtgatag catcaagctt gagaatgccg tgatactgct ttttgaattg atcttttaag
      361 atattttcag ttggcggttc gccatctgcc caggcattac cagtcatcaa cataagcagt
      421 gccgtgccaa caatccattg ctttttcacc cgaaaaacct cacgcggaat aatttattcc
      481 ggtgattatc attagggtaa tgaaaattgg caatggacgt aagtctgaag tgaaaaagcc
      541 ccggcacgat accggggcga ggtgattagt actgattgaa gatctgctgg atctgctgcg
      601 gatctttggt ttgcgtcaga gccagctgca gcaggacgcg cgctttttgc gggttcagcg
      661 tgccagaggc gacgaagccg tatttcgcat catccacttc agcatcctga gtggtagcac
      721 ccgtcggtac gcgggaagaa cgcactactg cagtgccgtt tttcgcggcg gttgccaggg
      781 tgtcgaacac ggatttatac aggttaccat tacccacacc agcgctaacg atgccatcat
      841 agcccgcatc taccagtgct ttagccggaa gatcggatgc gttagcgtag ttataaacga
      901 tgccgacttt cggcagctca ttcagcttag agacatcgaa tggcgtatcg ctggtgtgct
      961 tacgtgccgg ggtacgttgg tagtcaatct taccgttgtg aatgtatccc agaggaccgt
     1021 agttaacaga cttgaaggtc gctacgtcgg tggtgttggt tttggtgaca tcgcgaccgt
     1081 ccagtacggt gtcgttcatc accaccagca cgccacgatt agcggaggct ttatcagctg
     1141 cggtcactac cgcgttatac aggttgaatg gaccgtctgc gctcatggac gtggacgggc
     1201 gcattgcgcc gaccatcacc accggtttgt cgcatttcac cgtcaggtcg aggaagtaag
     1261 cggtttcttc catcgtgtcg gtaccgtggg taatgacgaa gccgtcggtt ttatcgcagt
     1321 cggtgttaat ttttttcgcc agtgtcagcc agacattatc gttcatgtcc tgggagccga
     1381 tattcactac ctgctcgcct ttaacgttcg caatgtcttt tagttgcggc accgcattaa
     1441 ccagattttc tacgccaact ttacccgctg tgtagttaga tttggttgcg gagtcaccac
     1501 caccggcaat ggtcccgccg gttgctaaaa tggtgatatt gggtaatgcc aatgctgcac
     1561 cactaaaacc cataaccagt gcggcaagtg ccgtcttttt gaaaaactcc atttcattcc
     1621 tccagttacg tgaacgctac gcattatccc ttagctctgt atgggaaatt tgacgttaaa
     1681 caatttacaa cgtgaatata ttttggagat ctacaaagtt agaggcaggt aacaaaacga
     1741 agaattaaac ggcataaaaa agtattatgc cgtcttaaaa tagaggatta ttttaaatta
     1801 ccgaccaggg ctttgcggct atcttccaga gtcacaacgc ggctacaaac atctttgcca
     1861 aactgctgga aatctttttc ctgctttttc cactctgttt ggattgagga ttgcagcccc
     1921 cccaggcttc ccagcacatt ctgtaatggg ttaccgccgc ttttcagcac cgctttcgcg
     1981 cccatttcat taatgctgtc ctgtaaaatt ccacccattg cctgattcac taattgctgg
     2041 ccttcggcgc gaacctgatc aatggcttta tagtgaaacg tcaggccatc gctgcgcgtt
     2101 tcgataatgc ggttcatctg ctctttcagc tgcgcatcaa gtttggtcag acggctgcgc
     2161 attttgctgc tttcgcccat ctcctgaacg ataattttat ccagcgcaat acgggctttt
     2221 tcgacgcggc ttttcgcgcc ttcatcaatc cacggcagcg tgctgcgtag ttcagcctga
     2281 taatccttcg cctgctcgcg ctgggcggca ttcagggaat attgcttacc gttatacatc
     2341 acgttgccgt ctggcgtgat caccagattg ccgttttcgc ccttcacctg cacggtttgc
     2401 gggctgacaa tcacatcgtc acgcggcgtg acgctgcact ggtagtcggc gtgagcggtc
     2461 attgccgtca ctgaaagtgc tgccgccagc agcattttgc gcatcatagt cttccctcaa
     2521 gaaaaaatca ggccagcatt tgctggcccc agattgataa caaagtacgc gttttccatg
     2581 ccggatgcgg cgtaaacacc ttatcccgcc tactcgatcg tgcatctttc caggcctgat
     2641 aagcgtagcg catcaggcag ttttgcattt gtcatcgccc gtatgctttc ttagtcccac
     2701 caaacgtcga aaagttcgct ggtgcgtacc tcatccagtt tgcgctcttc cagccactta
     2761 cgcacaatcg cctgatgttc ttcggtgcat ttgccgattt cctgcatgca gatcagacct
     2821 tcccaggcca gataaccgct gccgtcaaag gccagtttgt tcggttcgat aacctcgtta
     2881 ataaaatcat caacggtttt atcaatctgt tcttccgatg taccttccgg gaatcgccat
     2941 gccaccgaaa atcctaattc ctggaattcg tcgatgtgca tttttttacg cagacgacgg
     3001 ctacggttct ttgccattat ttcaccctct cgaacattaa gtcccatact ccgtgaccaa
     3061 gacgatgacc acgttgttca aatttcgtca ccggacgtga tgccggacgc ggcacgtaat
     3121 cattgctctc tgacaggttt ttataaccgt caatagaaga catcacttca agcatatgtt
     3181 ccgcataagg ttcccagtcg gtcgccatat ggaatacacc ccccagctgc agtttgcttt
     3241 ttaccagttc ggcaaacggc acctgtacga tacggcgttt attatggcgc gctttgtgcc
     3301 acgggtcagg gaaaaagagc tgcaccatgc gcaatgaatt gtcaggaatc attttatgca
     3361 gcacttcaac cgcatcgtga cacatcacgc gcaggttgct taaaccttct tcatgcgcag
     3421 aagccaggca cgcaccaacg cccggtgaat gcacttcaat gccgaggaag tcctgctcag
     3481 ggcgatcttt agccattgcc accagcgacg cccccatgcc aaaaccaatc tcaagcgtca
     3541 ccggcgcttc acggccaaaa agcgcgggga aatccagcat atcttcgctg aactcaacgc
     3601 ccatcaccgg ccagtagttt tccagcgcat gttcctggcc tttggtcagt cgcccctggc
     3661 ggcgcacaaa actacggata cggcgcagtg ggcggccgtt ttcatcaaat tccggtgaaa
     3721 tgacgtcgtt tttcataaag atttagtcgc ttgtgaaagt gttctgaaaa cgggcattat
     3781 ccaaagttag ttgccggatg caagcatgat aaggccgtgg ctgcggaaag ttccggttta
     3841 caccctgccg tcgctgtgct gcaatcttgc ccccaacaac agtgaattcg gtgaccatgc
     3901 aagcgtcgca attttcagcc caggttctgg actggtacga taaatacggg cgaaaaacgc
     3961 tgccctggca aattgacaag acgccctaca aagtatggct ctcagaagtg atgttgcaac
     4021 aaactcaggt tgcgaccgtt atcccctatt ttgaacgctt tatggcgcgc ttcccgacgg
     4081 tgaccgatct cgccaatgcg ccgctcgacg aagttctcca cttgtggacc gggcttggct
     4141 attacgcccg cgcgcgcaat ctgcataaag cggcacaaca agtggcgacc ttacacggcg
     4201 gtaaattccc ggaaaccttt gaagaagtcg cggcgttacc gggcgtcggg cgttccaccg
     4261 caggcgcgat tctctcgctt tctctgggta agcactttcc gattctcgac ggtaacgtca
     4321 aacgcgtgct ggcgcgctgc tatgctgtaa gcggctggcc tgggaaaaaa gaggtcgaga
     4381 ataaattatg gagtttgagc gagcaggtga cgcccgcggt cggcgtggaa cggtttaatc
     4441 aggcgatgat ggatttgggc gcgatgattt gtacgcgctc gaagccgaaa tgttcgctct
     4501 gtccgctaca aaacggatgt attgccgccg ccaataatag ctggtcgctt tatccgggca
     4561 aaaaaacgaa acagacgctg ccggagcgca ccggctactt tttgctgtta cagcacgaag
     4621 atgaagtatt gctggcgcag cgtccgccga gcggattgtg gggcggttta tactgtttcc
     4681 cgcagtttgc cgacgaagaa agtttgcggc agtggctggc gcaacggcag attgctgccg
     4741 ataacctgac gcaactgacc gcgtttcggc ataccttcag ccatttccac ttagatattg
     4801 tgcctatgtg gcttcccgtg tcgtcattca ccggctgcat ggatgaaggc aatgcgctct
     4861 ggtataactt agcgcaaccg ccgtcagttg gcctggcggc tcccgtggag cgtttgttac
     4921 agcagttacg cactggcgcg ccggtttagc gcgtgagtcg ataaagagga tgatttatga
     4981 gcagaacgat tttttgtact ttcctgcaac gtgaagcaga aggtcaggat tttcagctgt
     5041 accccggcga gctgggaaaa cgcatctata acgagatctc caaagaagcc tgggcgcagt
     5101 ggcagcacaa gcaaaccatg ctgattaatg aaaagaaact caacatgatg aatgccgagc
     5161 accgcaagct gcttgagcag gagatggtca acttcctgtt cgagggtaaa gaggtgcata
     5221 tcgagggcta tacgccggaa gataaaaaat aaaaacagtg ccggagcacg cctccggcaa
     5281 cttgcataaa aacaaacaca acacgcaccc ggaatgatga aaaaatatct cgcgctggct
     5341 ttgattgcgc cgttgctcat ctcctgttcg acgaccaaaa aaggcgatac ctataacgaa
     5401 gcctgggtca aagataccaa cggttttgat attctgatgg ggcaatttgc ccacaatatt
     5461 gagaacatct ggggattcaa agaggtggtg atcgccggtc ctaaggacta cgtgaaatac
     5521 accgatcaat atcagacccg cagccacatc aacttcgatg acggtacgat tactatcgaa
     5581 actatcgccg ggacagaacc tgccgcgcat ttgcgccggg caattatcaa aacgttgctg
     5641 atgggtgacg atccgagttc ggtcgatctc tattccgacg ttgatgatat tacgatttcg
     5701 aaagaacctt tcctttacgg tcaggtggtg gacaacaccg ggcagccgat tcgctgggaa
     5761 ggtcgcgcaa gcaacttcgc ggattatctg ctgaaaaacc gtctgcaaag ccgcagtaac
     5821 gggctgcgta tcatctacag cgtcaccatt aacatggtac cgaaccacct tgataaacgt
     5881 gcgcacaaat atctcggcat ggtccgccag gcgtcacgga aatatggcgt tgatgagtcg
     5941 ctgattctgg caattatgca gaccgaatct tcctttaacc cgtatgcggt cagccgttcc
     6001 gatgcgctgg gattaatgca ggtggtacaa catactgccg ggaaagatgt gttccgctcg
     6061 caggggaaat ccggcacgcc gagccgcagt ttcttgtttg atcctgccag caatattgat
     6121 accggcaccg cgtatctggc gatgctgaac aatgtttatc tcgacggaat tgataaccca
     6181 acatcgcggc gttatgccgt catcaccgcc tataacggcg gcgcaggcag cgtgctgcga
     6241 gtcttttcga atgataagat tcaggctgcc aatattatta acaccatgac gccgggcgat
     6301 gtttatcaga cgctgacgac ccgccatccc tctgcggaat ctcgccgtta tctttataaa
     6361 gtgaataccg cgcaaaaatc ctaccgccgc cgataattcc attaaccgcc cctgacgatg
     6421 ctcaggggca aaaatgttat ccacatcaca atttcgtttt gcaaattggg aatgtttgca
     6481 attatttgcc acaggtaaca aaaaaccagt ccgcgaagtt gatagaatcc catcatctcg
     6541 cacggtcaaa tgtgcttttt caaacactca tccgcatcac aatgtgagga aattaacatg
     6601 aatcttaagc tgcagctgaa aatcctctct tttctgcagt tctgtctgtg gggaagttgg
     6661 ctgacgaccc tcggctccta tatgtttgtt accctgaagt ttgacggtgc ttctattggc
     6721 gcagtttata gctcactggg tatcgccgcg gtatttatgc ctgcgctgct ggggattgtg
     6781 gccgacaaat ggttaagtgc gaaatgggta tatgccattt gccacaccat tggcgctatc
     6841 acgctgttca tggcggcaca ggtcacgacg ccggaagcga tgttccttgt gatattgatt
     6901 aactcgtttg cttatatgcc aacgcttggg ttaatcaaca ccatctctta ctatcgcctg
     6961 caaaatgccg ggatggatat cgttactgac ttcccgccaa tccgtatctg gggcaccatc
     7021 ggctttatca tggcaatgtg ggtggtgagc ctgtctggct tcgaattaag ccacatgcag
     7081 ctgtatattg gcgcagcact ttccgccatt ctggttctgt ttaccctgac tctgccgcat
     7141 attccggttg ctaaacagca agcgaatcag agctggacaa ccctgctggg cctcgatgca
     7201 ttcgcgctgt ttaaaaacaa gcgtatggca atcttcttca tcttctcaat gctgctgggc
     7261 gcggaactgc agattaccaa catgttcggt aataccttcc tgcacagctt cgacaaagat
     7321 ccgatgtttg ccagcagctt catcgtgcag catgcgtcaa tcatcatgtc gatttcgcag
     7381 atctctgaaa ccctgttcat tctgaccatc ccgttcttct taagccgcta cggtattaag
     7441 aacgtaatga tgatcagtat tgtggcgtgg atcctgcgtt ttgcgctgtt tgcttacggc
     7501 gacccgactc cgttcggtac tgtactgctg gtactgtcga tgatcgttta cggttgcgca
     7561 ttcgacttct tcaacatctc tggttcggtg tttgtcgaaa aagaagttag cccggcaatt
     7621 cgcgccagtg cacaagggat gttcctgatg atgactaacg gcttcggctg tatcctcggc
     7681 ggcatcgtga gcggtaaagt tgttgagatg tacacccaaa acggcattac cgactggcag
     7741 accgtatggt tgattttcgc tggttattcc gtggttctgg ccttcgcgtt catggcgatg
     7801 ttcaaatata aacacgttcg tgtcccgaca ggcacacaga cggttagcca ctaattacgc
     7861 aaagaaaaac gggtcgccag aaggtgaccc gtttttttat tcttacttca acacataacc
     7921 gtacaatcgt ttcatgccat ccgcatcggt ttcgctatag acaccttgca actccggcga
     7981 aaatcccggc aacaaattca cgccttcttc cagcgcaagg aaataacgtt gaaccgcccc
     8041 accccagact tccccgggaa ccacgcaaag cacgccaggt ggataaggca acgccccttc
     8101 tgccgcaatt cgcccttcgg catcacgaat ccgcaccaac tccacttcac cgcgaat
//
LOCUS       DAJVQG010000160         8145 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJVQG010000160 DAJVQG010000000
VERSION     DAJVQG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8145
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.160"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            87..398
                     /locus_tag="OQM83_003427"
     CDS             87..398
                     /locus_tag="OQM83_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1521608.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            493..1026
                     /gene="ymdB"
                     /locus_tag="OQM83_003428"
     CDS             493..1026
                     /gene="ymdB"
                     /locus_tag="OQM83_003428"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCS1521609.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            968..2449
                     /gene="clsC"
                     /locus_tag="OQM83_003429"
     CDS             968..2449
                     /gene="clsC"
                     /locus_tag="OQM83_003429"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCS1521610.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2457..3614)
                     /gene="mdoC"
                     /locus_tag="OQM83_003430"
     CDS             complement(2457..3614)
                     /gene="mdoC"
                     /locus_tag="OQM83_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCS1521611.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4009..5544
                     /gene="mdoG"
                     /locus_tag="OQM83_003431"
     CDS             4009..5544
                     /gene="mdoG"
                     /locus_tag="OQM83_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCS1521612.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5537..8080
                     /gene="mdoH"
                     /locus_tag="OQM83_003432"
     CDS             5537..8080
                     /gene="mdoH"
                     /locus_tag="OQM83_003432"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCS1521613.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2034 a         1946 c         2142 g         2023 t
ORIGIN      
        1 gagaatggtc tgctgcgaat cagccaacct gaaagtatgg ataacacaac cctcaaggat
       61 gactaatcat tgaggaaata gaataaatgt tcagaccttt tttaaactct cttatgctcg
      121 gcagtttgtt ttttcctttt attgccatcg caggaagcac cgcgcaaggg ggtgtgatcc
      181 atttttatgg ccaaattgtg gaaccggcat gtgacgtcag cacccagtca tcacccgtag
      241 aaatgaactg cccacagaat ggttctgttc cgggcaaaac ctattccagc aaagcgttaa
      301 tgagcggtaa tgttaaaaac gcgcaaatag cctcagtaaa agtgcagtat cttgataaac
      361 agaaaaagct ggctgtaatg aacatcgaat ataactaagt ttctgaacaa cgcacgttgc
      421 tgagcagaaa aatgcgattt aaccaaaaag cctgctgtac acttaagaaa caagaaggtg
      481 taaggaggcg ttatgaaaac gcgtattcat gttgtgcagg gtgatattac caaactggcc
      541 gttgatgtga ttgtgaatgc ggctaatccg tcattaatgg gaggcggcgg cgtcgatggg
      601 gccattcatc gcgcagcggg tccggccctg ctggatgcct gtttaaaagt caggcaacag
      661 cagggcgatt gccctacggg gcatgccgtt attacgcttg caggcgatct tcccgctaaa
      721 gccgtagtac acaccgtcgg gccagtctgg cgtggtggtg aacaaaacga agaccagctt
      781 ttgcaggatg cctatctgaa tagcctgcga ctggtggcgg caaacagcta tacgtcagtg
      841 gcttttcctg caatcagtac tggggtttat ggttaccctc gtgcggcagc ggctgaaatc
      901 gcagttaaaa ccgtttcaga atttattacc cgtcacgctt tacccgaaca ggtatacttt
      961 gtctgttatg atgaagaaaa cgcccacctc tacgaaagac tccttaccca acaaggagat
     1021 gaatgatttg ccccggctgg cgagcgcggt gctgccactg tgttcgcaac atcccggtca
     1081 gtgtggtctt ttccctctgg agaaaagtct ggatgcgttt gccgcccggt atcgtctggc
     1141 cgaaatggca gagcatacgc tggatgttca gtattacatc tggcaggacg atatgtcggg
     1201 tcggttactg ttttccgccc tgttagccgc agcaaagcgt ggcgttcgcg tccgtttgtt
     1261 gctggacgac aacaatacgc ccggacttga cgatatttta cgcttgcttg acagccatcc
     1321 ccgcattgaa gtccggcttt ttaatccttt ctcgtttcgc ttgctgcgtc cgcttggtta
     1381 tatcaccgac ttttcccgtc tcaatcgccg tatgcacaat aaaagtttca ctgtcgatgg
     1441 cgtggttacc ctggtgggag gacgaaatat tggtgatgcc tattttgggg caggggaaga
     1501 gccacttttt tcggatttag atgttatggc aataggaccc gtggtagagg acgttgccga
     1561 tgatttcgcc cgctactggt attgcaaatc ggtttcaccc ttacagcagg tgctggatgt
     1621 cccggaaggt gaaatggcgg atcgcatcga gttacccgcc tcctggcata acgatgccat
     1681 gacgcatcgt tacttacgcc aaatggaatc cagtccgttt ataaatcatc tggttgatgg
     1741 aacgttgccg cttatctggg cgaaaacacg tttattaagt gatgatccgg cgaaagggga
     1801 gggcaaggca aaacggcatt cactgttacc gcagcgcctg tccgatatca tgggctcacc
     1861 cagtgaacgc atcgatatta tctcttccta ttttgtaccg acacgcgcag gtgtggcgca
     1921 actcttacgg atggtgagaa aaggggtaaa gattgcgatc ctaaccaatt ctcttgccgc
     1981 taacgatgtt gctgtcgtcc atgccggata cgcgcgctgg cgcaaaaaat tgctccgcta
     2041 tggcgtggaa ttatatgaac tcaagccgac gcgtgaacaa agtagtacgt tacacgatcg
     2101 cggcataacc ggtaattccg gagccagcct gcatgctaaa acttttagca tcgatggtaa
     2161 aacggtgttt atcggctctt tcaatttcga tccgcgttca acattgctca atactgaaat
     2221 gggcttcgtg atagagagcg aaacgctggc acagttaatt gataaacgct ttattcagag
     2281 ccagtatgat gcggcctggc agctccgtct ggacaggtgg ggacggatca actgggttga
     2341 tcgtcatgca aagaaagaga ttattctcaa aaaagaaccc gccaccagtt tctggaagcg
     2401 ggttatggtc agactggcgt cgatattgcc cgtggaatgg ttattgtaaa tgtggcttaa
     2461 cgggctggtg ctttatcgtt ctcacgctta acaaccggtt taccagaaaa caaaaacttc
     2521 agtaacggga tgcgtaaatg aatttcatac agaattatcg caatccctac tacgaatatc
     2581 aggccacaga gaaaaccaag ccagttggag gtgatgtgcg gtgtaatgta tgcgccgaaa
     2641 aacagcgtta acgggtggtg aaccagatag ataaatagcg atgcgttaac aaagtaagtc
     2701 acccgcgctg actggaagtt aagcaaacgg tggccaaagg agaagaccac attcaccatc
     2761 cacagaccga ggaccatggt gatcaccgac tcggtttcgt acatccaggc atcgccactg
     2821 ccatagcgct ggttgagtaa ataagcgaca aatgccaatg ctgctgcaag ggtacagcca
     2881 cgagacggcg tggtaaacaa ggctttaaga tgagggaaaa tgaaagccag tgcaccgagg
     2941 ataaagaacg gcaaataaaa tagcgtttgc atgacaataa aattgaacat gccattactc
     3001 agaatgggcg gatacacaat aaaaatcgtt cttcttatta ccgcatagcc gatgccgagg
     3061 cataaaaaaa tcaccgatag ttttaccatc gagaattttt tattcgtttt atcagaattt
     3121 tctaaattat ttctgatgcg tttaaatatc catacgcaca gcgtcgtcat gactactaac
     3181 accagtaaaa accacaggtg tgatattaat tcccaggcca acgtattata tttgtcatac
     3241 aatgacagcc caggccaact ttccgctttc cctttgacgt attgcagcat aataaattgc
     3301 ggcaatgtca gtaaggggat ggctgttaac atcgggatac ctacacgttc gacacgtact
     3361 ttccaccatt ttttcaaggg atagcgtaaa aaaagcatgt aggaaaagta gccggatata
     3421 acgaaaaata cctgcatgcg gaacgagtgg atgaagtcat taaaaagggt cagccacaat
     3481 gacggttcgg cgctattcac atgccatgta tggctcgaat agattaaaga aatatgaaaa
     3541 gggatcccta acaacatcag ccaggcgcgg atggagtcga ggaaatattc acgttgcgcg
     3601 ggtactggat tcatatatgg ttaactaatc tcggattttt cgtcttatcc ctgtcgggtt
     3661 atgcctttag gcttgttgcc atagcgacac cgacctgacc gcgccaggcg caggcttcaa
     3721 ggtttttatg catagcatca tcgctaccac taaccagaat ggaagcgtct gtaagacggt
     3781 tgataaataa aattgctggc aaaccctaca cgaagtcgat gcttctgtct ttaggagaag
     3841 cacggaaagt gaaaacggtt gcaatcaggt gcttaatcca tgagccagcg tgctgaacga
     3901 taccgggatt ctgttgtcgg aatggctggt tatccattaa aatagatcgg atcgatataa
     3961 gcacacaaag ggggaagtgc tttactaatt atgaaacata aactacaaat gatgaaaatg
     4021 cgttggttga gtgctgcagt aatgttaacc ctgtatacat cttcaagctg ggctttcagt
     4081 attgatgatg tcgcaaagca agctcaatcc ttagccggga aaggctatga ggcgcccaaa
     4141 agcaacttgc cctccgtttt ccgcgatatg aaatacgcgg actatcagca gatccagttt
     4201 aatcatgaca aagcgtactg gaacaatctg aagaccccat tcaaactcga gttctaccat
     4261 cagggtatgt acttcgatac cccggtcaaa ataaatgaag tgactgccac cgcagtcaaa
     4321 cgaatcaaat acagcccgga ttatttcact ttcggcgatg ttcagcatga caaagacacg
     4381 gtaaaagacc ttggttttgc cggttttaaa gtgctttacc cgatcaacag caaagataaa
     4441 aacgatgaaa tcgtcagcat gctcggggcc agctatttcc gcgtgattgg tgcaggtcag
     4501 gtttatggcc tttctgcccg cggcctggca attgataccg ccttgccatc gggggaagaa
     4561 tttccacgct tcaaagagtt ctggatcgag cgtccaaaac cgactgataa acgtttaacc
     4621 atttatgcat tgcttgactc gccgcgcgcg acaggtgctt acaaattcgt ggttatgcca
     4681 gggcgtgaca cggttgtgga tgtgcagtcg aaaatctatc tgcgcgataa agtcggcaaa
     4741 ctgggggttg caccgttaac cagtatgttc ctgtttgggc cgaaccaacc gtcgcctgca
     4801 aataactatc gtccggagtt gcacgactct aacggtctct ctatccatgc cggtaatggc
     4861 gaatggatct ggcgtccgtt gaataacccg aaacatttag cggttagcag cttctcgatg
     4921 gaaaacccgc aaggcttcgg tctgttgcag cgcggtcgtg atttctcccg ctttgaagat
     4981 ctcgatgatc gttacgatct tcgtccaagc gcatgggtga ctccgaaagg ggagtggggt
     5041 aaaggcagcg ttgagctggt ggaaattcca accaacgatg aaaccaacga taacatcgtc
     5101 gcttactgga cgccggatca gctgccggag ccgggtaaag agatgaactt taaatacacc
     5161 atcaccttca gccgtgatga agacaaactg catgcgccag ataacgcatg ggtgcaacaa
     5221 acgcgtcgtt caacggggga tgtgaagcag tcgaacctga ttcgccagcc tgacggtact
     5281 atcgcctttg tggtcgattt taccggcgca gagatgaaaa aactgccaga ggataccccg
     5341 gtcacagcgc aaaccagcat tggtgataat ggtgagatag ttgaaagcac ggtgcgctat
     5401 aacccggtta ccaaaggctg gcgtctggtg atgcgtgtga aagtgaaaga tgccaagaaa
     5461 accactgaaa tgcgtgctgc gctggtgaat gccgatcaga cgttgagtga aacctggagc
     5521 taccagttac ctgccaatga ataagacaac tgagtacatt gacgcaatgc ccatcgccgc
     5581 aagcgagaaa gcggcattgc cgaagactga tatccgcgcc gttcatcagg cgctggatgc
     5641 cgaacaccgc acctgggcgc gggaggatga ttccccgcaa ggctcggtaa aggcgcgtct
     5701 ggaacaagcc tggccagatt cacttgctga tggacagtta attaaagacg acgaagggcg
     5761 cgatcagctg aaggcgatgc cagaagcaaa acgctcctcg atgtttcccg acccgtggcg
     5821 taccaacccg gtaggccgtt tctgggatcg cctgcgtgga cgcgatgtca cgccgcgcta
     5881 tctggctcgt ttgaccaaag aagagcagga gagcgagcaa aagtggcgta ccgtcggtac
     5941 catccgccgt tacattctgt tgatcctgac gctcgcgcaa actgtcgtcg cgacctggta
     6001 tatgaagacc attcttcctt atcagggttg ggcgctgatt aatcctatgg atatggttgg
     6061 tcaggatttg tgggtttcct ttatgcagct tctgccttat atgctgcaaa ccggtatcct
     6121 gatcctcttt gcggtactgt tctgttgggt gtccgccgga ttctggacgg cgttaatggg
     6181 cttcctgcaa ctgcttattg gtcgcgataa atacagtata tctgcgtcaa cagttggcga
     6241 tgaaccatta aacccggagc atcgcacggc gttgatcatg cctatctgta acgaagacgt
     6301 gaaccgtgtt tttgctggcc tgcgtgcaac gtgggaatca gtaaaagcca ccgggaatgc
     6361 caaacacttt gatgtctaca ttcttagtga cagttataac ccggatatct gcgtcgcaga
     6421 gcaaaaagcc tggatggagc ttatcgctga agtcggtggc gaaggtcaga ttttctatcg
     6481 ccgccgccgt cgccgcgtga agcgtaaaag cggtaatatc gatgacttct gccgtcgctg
     6541 gggcagccag tacagctaca tggtggtgct ggatgctgac tcggtaatga ccggtgattg
     6601 tttgtgcggg ctggtgcgcc tgatggaagc caacccgaac gccgggatca ttcagtcgtc
     6661 gccgaaagcg tccggtatgg atacgctgta tgcgcgctgt cagcagttcg cgacccgcgt
     6721 gtatgggcca ctgtttacag ccggtttgca cttctggcaa cttggcgagt cgcactactg
     6781 gggacataac gcgattatcc gcgtgaaacc gtttatcgag cactgcgcac tggctccgct
     6841 gccgggcgaa ggttcctttg ccggttcaat cctgtcacat gacttcgtgg aagcggcgtt
     6901 gatgcgccgt gcaggttggg gggtctggat tgcttacgat ctcccgggtt cttatgaaga
     6961 attgccgcct aacttgcttg atgagctaaa acgtgaccgc cgatggtgcc acggtaacct
     7021 gatgaacttc cgtctgttcc tggtgaaggg tatgcacccg gttcaccgtg cggtgttcct
     7081 gacgggcgtg atgtcttatc tctccgctcc gctgtggttt atgttcctcg cgctctctac
     7141 tgcattgcag gtagtacatg cgttgaccga accgcaatac ttcctgcaac cacggcagtt
     7201 gttcccggtg tggccgcagt ggcgtcctga gctggcgatt gcactttttg cttcgaccat
     7261 ggtgctgttg ttcctgccga agttattgag cattttgctt atctggtgca aaggaacgaa
     7321 agaatacggc ggcttctggc gcgttacatt atcgttgctg ctggaagtgc tgttttccgt
     7381 gctgctggct ccggtacgca tgctgttcca tacggtcttc gtcgtcagcg cgttccttgg
     7441 ctgggaagtg gtgtggaatt caccgcagcg tgatgatgac tccacttcct ggggtgaagc
     7501 gttcaaacgc cacggctcac agctgctgtt agggttagtg tgggctgttg ggatggcgtg
     7561 gctggatctg cgtttcctgt tctggctggc accgattgtc ttctcgttga tcctgtcacc
     7621 gtttgtttcg gtgatttcca gccgtgccac cgttggtctg cgcaccaaac gctggaaact
     7681 gttcctgatc ccggaagagt attcaccgcc gcaggtgctg gttgataccg atcggttcct
     7741 tgagatgaat cgccaacgct cccttgacga tggctttatg cacgcagtgt ttaacccgtc
     7801 atttaacgct ctggcaaccg caatggcgac cgcgcgtcac cgcgccagta aggtgctgga
     7861 aatcgcccgt gaccgccacg ttgaacaggc gctgaacgag acgccagaga agctgaatcg
     7921 cgatcgtcgc cttgtgctgc taagcgatcc ggtgacgatg gcccgtctgc atttccgcgt
     7981 ctggaattcc cctgagagat attcttcatg ggtgagttat tacgaaggga taaagctcaa
     8041 tccactggca ttgcgtaaac cggatgcggc ttcgcaataa aaatgtagtt gcctgatgcg
     8101 ctacgcttat caggcctaca tcgttcatgc aatttattga ttttg
//
LOCUS       DAJVQG010000161         8106 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJVQG010000161 DAJVQG010000000
VERSION     DAJVQG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.161"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..1341
                     /gene="yjeM"
                     /locus_tag="OQM83_003433"
     CDS             <1..1341
                     /gene="yjeM"
                     /locus_tag="OQM83_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCS1521614.1"
                     /translation="LMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIW
                     MVSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKI
                     ARITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVV
                     FAIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVL
                     SNGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFT
                     LCYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASA
                     FFNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVT
                     FANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(1492..4815)
                     /gene="mscM"
                     /locus_tag="OQM83_003434"
     CDS             complement(1492..4815)
                     /gene="mscM"
                     /locus_tag="OQM83_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCS1521615.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(4837..5805)
                     /gene="psd"
                     /locus_tag="OQM83_003435"
     CDS             complement(4837..5805)
                     /gene="psd"
                     /locus_tag="OQM83_003435"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCS1521616.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5902..6954)
                     /gene="rsgA"
                     /locus_tag="OQM83_003436"
     CDS             complement(5902..6954)
                     /gene="rsgA"
                     /locus_tag="OQM83_003436"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCS1521617.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7049..7594
                     /gene="orn"
                     /locus_tag="OQM83_003437"
     CDS             7049..7594
                     /gene="orn"
                     /locus_tag="OQM83_003437"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCS1521618.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7805..7880
                     /locus_tag="OQM83_003438"
     tRNA            7805..7880
                     /locus_tag="OQM83_003438"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7838..7840,aa:Gly,seq:gcc)
BASE COUNT         1864 a         2097 c         2186 g         1959 t
ORIGIN      
        1 ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat ctattcctgg
       61 atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg gttttcctct
      121 tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc aacattcctc
      181 tatggtagcg acatgaccca gcactggcgt attgccggac tggagcctac gcaggtggtt
      241 ggtctgctgg cagtagcatg gatgattctg gtcaccgtcg ttgcttcaaa ggggattaat
      301 aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa tttagtattg
      361 ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca ggatattaat
      421 ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc gtttgtggta
      481 tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga taaaacggaa
      541 aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat ttcaatcggt
      601 tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt attaagtaat
      661 ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg ggtgacgctg
      721 ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg gtttgcgcgt
      781 attaccggac tttcgatgtt cctcgcttat accggtgcgt tctttacgct ttgctattca
      841 ccgctgaaag ccatcatcca ggggacgccg aaagcgttgt ggccggaacc gatgacgcgc
      901 ctgaatgcga tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt ggttactgtc
      961 ttcatcctgc tggtttcgtt tggtggcggt accgcatccg cgttctttaa caagctgacg
     1021 ctgatggcga acgtgtctat gacgcttcct tacctgttcc tcgcgctggc tttcccattc
     1081 tttaaagcac gtcaggatct cgacagacca tttgtgattt tcaaaacgcg tatgtcggca
     1141 atgattgcga tggtggttgt cgtactggtg gtgacatttg cgaacgtctt caccattatt
     1201 cagcctgtgg ttgaagccgg agactgggac agcacattgt ggatgattgg cggccctgtc
     1261 ttcttctcgc tgttagcgat ggcgatttac cagaactatt gcagtcgcat ggcgaataaa
     1321 cctgagttag ctctcgactg atcatatgaa atcctgcaac tcatcgtctc tccggcgatg
     1381 agttgtcagg aagtaatgcg tatggatgat actctccagg gccggtgata actgaagatg
     1441 atgttaagct gctaaatgag ccacgttgac atcatttggc agcttcatgc attacaaact
     1501 tcccgcctga cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt taccgttgag
     1561 gctttccaga cgcatctgga agggcgggaa tggcatatcg ataccgtggg catggaagcc
     1621 agccagaatc agctggtgga tctcatggcg tagcggcata cggtgaccca tctcagcggc
     1681 ataaatacgc agctcgaaaa tctgaatccc ttgttgcaga tccaccagga agacttccgg
     1741 tgccgggttg tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga tttccgtcac
     1801 ttcttcactg ttggcatcag caggggccgg aatcgtcaac actacgcgcg tgaccgagtc
     1861 agagagcgac cagttgataa actgctcggt aataaacgcc ttgttcggca cgattatctc
     1921 tttacggtcc cagtcgctga tggtggtggc gcgggtgtta attttcgtca cgctaccggt
     1981 gagatcgcga attgtcaccg tatcgccaat gcgaatcggt ttttcgaaca ggataatcag
     2041 accagagata aagttggcga aaatttcctg caaaccaaaa ccgagaccaa caccgagcgc
     2101 ggcgaccagc cactgcaatt tcgaccactc aataccaatc atcgagaagc cgaccagccc
     2161 gccaatcagc atcagcagat atttggtgat ggtggtgatg gcgtagcccg tacccggcgt
     2221 taaatccagg tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc gcaccagctg
     2281 cgtggtgatg ataaacacca gaatggcaat cagcaccgca ccgagggtaa ttggctccag
     2341 actttctacg ccctgtaccg tggaggtgac atcccacagc gaaatatttt cgaggaagcc
     2401 gaaagcggaa tggatttctg accacagcac aatgacagaa agcagggcga tcaacatcaa
     2461 aattgagcgc accagccgca aggattgcgc actgatggca tcgagatcga cttcgctttc
     2521 atcgacttca attgctcctt ccgggctact gtgatgatgc gcttcctctt cgccacgcgc
     2581 acgttgcgct aacatctctg cccggcgatg cttcgcccgg tcaaacgcca gcctgcggcg
     2641 ctggatcagc atccagcggc ggataacgtg ataaaccacc agtagcagga accagatggc
     2701 aaccgaggtt tcaagcctcg ctaacagcgc ctgtgccgtt gccagataac ccacagccga
     2761 cgccagaatg gcaaccaacg gcgcgccaat catcatgttc cacagcatat ggttggtaat
     2821 gttgtcgccg ctgccttctt tgttgagata cagcggaatc ccggcctttt tcaggctgag
     2881 ggtgaccacc gccagcgcac cgcaaatgag gataaagcaa agccttccca gcgaaccgga
     2941 gaattcacgg tcgtcgaggt tatcgaacat catcagcgcc ataatcagcg gcacaataag
     3001 cccgatgctc atcaggtagt agcgcatccc acgagatacg cgttcgcgcg gccagccaaa
     3061 atgagcgata aacaagccgt tcgggcgggc aaaggtggcg caaatcatca ctacccacag
     3121 cagcggcacg gtggccgtta caccatcgcc aatcgcgacc gccagcggat aaggccacgc
     3181 ctcgcgcaag ccgtaaccca gcgtcatcca cagcaccggt aacggtgacg cgacgagaat
     3241 cgaccagaaa agagtgcgca acgtcagcca gaagtgatcc tgagtcactt tgccaacttt
     3301 cgccgccgaa cgttcaagaa aacgggtgaa atagcggcgc gagtaaatac tgcaaccgac
     3361 cagaatcaac gcgccaaaca gcggcaaaat tgtctctttg ctggtcagca tcatcacact
     3421 ggctttgccc aactgactga aggtgtccag cgaaatgaga cgacgcagat cctgggcgat
     3481 ttccagcggc caggcgatgg tcatcggacg cacgtcagag gtccagaaca gatagcggtg
     3541 cgttgcttcg ttcacctctt tcagcgcatc ctccagttgc ccgttggaga ctttcagctt
     3601 ggtcagttcc aatagtagcg tgtcgccacc ctgcaacaat gagttcagca actcacgctg
     3661 agtgcgcagt tgtgcttcca gaatacggtt ttgctcggcg gtcagcggct gaccgtcggc
     3721 ctgatggatt tgccgtagca gcggctgttt attaagcaga tcctcataac gtaaccgttg
     3781 cacacgcaac tgcgccattt cggtatcaag ctgttgtggt tttggcattt ctggtagccg
     3841 tgccacctgc gctcgcaacg cttcaccgag cagattggac gatcccagcc attgcgactg
     3901 ttcacgcagc gtattcaacg cctgccggac ctgtaacgtc tggctggcag cctgacgctg
     3961 ttgcgaggca acgagatcca tccgctgcgc ctgttgattc agcgctgccg atagttcgcg
     4021 gttaattttg aattgcgcga cgatatcttt cggcaaatcg gcactgtttt ctgccagtaa
     4081 ttcggtactt tccagcgccc gctccgcctc aagctgacgt tggctattca actgattacg
     4141 taaggcctgc aaatacgcat ccagttgctg gctctctttt tccgcaagct ctgagcgtaa
     4201 gcgcgctaat tcttggcggt tattggcaga cagctgcgcc agctccagtt catcaacgag
     4261 cgccttaaga cgtgcagagt cagactgcaa cgcgaaattt tgtgcctgat tgagcggagt
     4321 attgccggta agcgttccca ggcggcgctc gatctcattt aactgacggc gggcgtcggt
     4381 ttgctgttgc ggcagttgat tcagcgaatc ggcaatctcg cgggcgcgct cctgctcttg
     4441 ctgggcctga cggcttttat ccagcaactg gctgctgacc tggagaattt cctgattcag
     4501 cgcgtcggta gacattcccg gcgacacgct gcgcggcttg tcacgcatgt tgtttaattg
     4561 tgcgcgcaga gtagcggaga gtttcggata attatcgata acttgctgat attgtttgat
     4621 gcgctcaagg gaaccttttc gttcctcaag cgcatttaag gcagactgaa gcgcctctac
     4681 gacttccggc tgtgcgggtt tcgccgcttt tgcctgctcc agttcctgag tgatttgttt
     4741 gctatcgggg gccgtcgcgg cgtacgcccc ccaactgagg caccaggcca tcagaaaagt
     4801 gataatcagg cgcacgtcag cgtttccttt gatggattag acctggtctt ttttgtcgtc
     4861 aaccaatggg ctggcgtcgt gttctgcttc gatctcttca gcaggaagcg gggcaggttc
     4921 agcgtctggc gtaacaaagg tttcggtaga tactgccagc ggctggcaaa ttttcgtgac
     4981 agacaggctt tccagttgct caaccagatt cactttaccc ggtgcaaaca ggttgataac
     5041 ggtggaaccg agtttaaagc gacccatttc ctggcctttc agcagtgcca cagaaccgtc
     5101 gttttccccg gcaggccagg tccagcgctt gatgatacct tcgcgcggcg gcgtaatggt
     5161 gcccgcccag accgtctcaa tgctgccaac aatcgtcgct ccgaccagaa tctgcgccat
     5221 tgggccaaat tcggtatcga aaaggcaaat cacgcgttcg ttacgggcaa acagattcgg
     5281 cacgttctga gccgtgagat ggttaacgga gaagagatcg cccggcacgt agatcatctc
     5341 acgcagaata ccgttgcacg gcatgtgtac gcggtggtag tcacgcgggg agaggtaagt
     5401 ggtcacaaac gtaccgttgc ggaacaggtc cgccatcaga tagttgcctg ccagcagggc
     5461 ttcgaggctg tagttgtggc ctttggcttg caggatttta tcttcttcga ttttacccaa
     5521 ctggctgata acgccatcgg caggcatgac cagtacattc ggatcggtat cgattgggcg
     5581 tacttcgtca cgcagcggac ggacaaagaa ttcgttaaag gtgcggtagc tggcggtgtc
     5641 cggcttttgc gcctctttca tgtcgacctt gtagtattta acgaacagat cgataaccag
     5701 ttttgtcaac catcctgccc gcttgcttgc gccccaaccc gccaggcgag taagccatag
     5761 tttcggcaga atgtactgta gcgaaagttt aaatgaattt aacaaggtag cctccaggcc
     5821 attgttttgt cgttcctgat tcggcctaca tgccggatcc tgaaaaaaag gggacgattc
     5881 taacgacggt tagcttaatt gtcagtcatc cgtatcagaa aagtttttac gcgtttttac
     5941 ctgcgccatg ctttccagaa tacggtgata gttttcgaaa cgggtttccg cgattttccc
     6001 ttcctcaacc gcttcccgga tagcgcagcc cggatcggta tcgtgtttgc aatcgcgata
     6061 tttacacaga cctaaatagt catggaattc gacaaagccc tgagtgattt gttccggctc
     6121 caggtgccag aggccgaact cacgcacacc aggggaatca atcacatcac cgccgtgcgg
     6181 gaagtgatac agccgagcgg cggtcgtggt gtgctgacca agccccgagt tgtcagagac
     6241 atcgttggtc aggatctctt tttgcagccc cagcaacgcg ttcagcaggc tggatttgcc
     6301 gacgccagac tgcccggcaa aaatgctgat gcgcccggtc aacgcctctt ccagcggttt
     6361 tagcccatcc tgagtatggc tggaaaccat caatacgcga taaccgatat tgcggtagat
     6421 atccatctgc tcgttgacga acgccatgcc ttcgtcgtcc agcaggtcta tcttgttgag
     6481 cacaataatc ggctcaatct gcaaggtttc gcaggccact aggtaacggt cgataatatt
     6541 gagcgacagc tccggcaaaa tggcggagac aatgacaatc tggtcgatgt tggcggcaat
     6601 aggttttacg ccgtcgtaga aatccggacg cgtcaacacc gaggtacgct catgcaccgc
     6661 ttccacgatc cctttgacat ttacgccttc cgccgccggt ttacccggac gccagactac
     6721 gcggtcgccg gttaccagcg aacggatggt acggcgaata ttgcagcggt gaacgtcgcc
     6781 atcggcggat tccacatcag cgtgcatacc aaagcggctg atgacgatac cttcatcagg
     6841 ctcgccaaac agattgtcgt cgtagtcggg cttctcctta gacgttttaa gacgacgctg
     6901 gtgattggcg ttcacgcggc gctgctggcc tttggagagt ttatttttac tcaatcgtac
     6961 tgactcctgg tcgcccctga tgggcaaaac atctatgata cacgcaattg tggatcaata
     7021 tagtcactgt gaatgggtgg aaaatagcat gagtgccaat gaaaacaacc tgatttggat
     7081 cgatcttgag atgaccggtc tggatcccga gcgcgatcgc attattgaga ttgccacgct
     7141 ggtgaccgat gccaatctga atattctggc agaagggccg accattgcag tacatcagtc
     7201 cgatgaacag ctggcgctga tggatgactg gaacgtgcgc acccataccg ccagcgggct
     7261 ggtagagcgc gtgaaagcga gcacgatggg cgatcgggaa gctgaactgg caacgctcga
     7321 attcttaaaa cagtgggtgc ctgcgggaaa atcgccgatt tgcggtaaca gcatcggaca
     7381 ggaccgtcgt ttcctgttta aatacatgcc ggagctggaa gcctacttcc actaccgtta
     7441 tctcgatgtc agcaccctga aagagctggc gcgccgctgg aagccggaaa ttctggatgg
     7501 ttttaccaag caggggacgc atcaggcaat ggatgatatc cgtgaatcgg tggcggagct
     7561 ggcttactac cgcgagcatt ttatcaagct gtaaaatttt tcctggtcac gtaagcgccg
     7621 gaatccactt gcggcggcgt gattttgacg ctaaattgaa cgtcttgctg ataatttcag
     7681 cacttgagat aaaaacgcaa aaaaaacttt tttggggggt tgcagaggga aagatttctc
     7741 gtataatgcg cctcccgtaa cgacgcagaa atgcgaaaat tacgaaagca aaattaagta
     7801 gtacgcggga atagctcagt tggtagagca cgaccttgcc aaggtcgggg tcgcgagttc
     7861 gagtctcgtt tcccgctcca aaatttgaaa agtgctgcaa agcacagacc acccaatcgc
     7921 agtatttcga attgcgacaa ggcggcaact gaacgaatcc ttgggaactt acaaaagtat
     7981 gtgactaagg tgagtgaagg cagccaaggc agtagcggtt cgaaagacga agattaagcg
     8041 ggaatagctc agttggtaga gcacgacctt gccaaggtcg gggtcgcgag ttcgagtctc
     8101 gtttcc
//
LOCUS       DAJVQG010000162         8104 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJVQG010000162 DAJVQG010000000
VERSION     DAJVQG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.162"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(101..796)
                     /locus_tag="OQM83_003439"
     CDS             complement(101..796)
                     /locus_tag="OQM83_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCS1521619.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(789..2216)
                     /locus_tag="OQM83_003440"
     CDS             complement(789..2216)
                     /locus_tag="OQM83_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCS1521620.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2227..2946)
                     /locus_tag="OQM83_003441"
     CDS             complement(2227..2946)
                     /locus_tag="OQM83_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCS1521621.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="OQM83_003442"
     CDS             complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="OQM83_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HCS1521622.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            4554..5879
                     /locus_tag="OQM83_003443"
     CDS             4554..5879
                     /locus_tag="OQM83_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HCS1521623.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            5988..6224
                     /locus_tag="OQM83_003444"
     CDS             5988..6224
                     /locus_tag="OQM83_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1521624.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6236..6829
                     /gene="rclC"
                     /locus_tag="OQM83_003445"
     CDS             6236..6829
                     /gene="rclC"
                     /locus_tag="OQM83_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HCS1521625.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(6989..7858)
                     /locus_tag="OQM83_003446"
     CDS             complement(6989..7858)
                     /locus_tag="OQM83_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCS1521626.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
BASE COUNT         2193 a         1982 c         1732 g         2197 t
ORIGIN      
        1 agcataacca tgacggaacg gggttaagtc aggttgtcct taacgctatt ttcaacctcg
       61 tatgccttct tcaggtttat gttcagatct catatctctc tcagcaatcc tcaataatca
      121 gatacaccgc tttcaccgga ccatgaaccc cgacgacttt gataagctca atatccgccg
      181 ttgagctggg accactgatg atgttaatac atgacggcat tcgttcaccg gcctgcgctt
      241 tctgatgcaa tttttctgcg agttgcgcta cacgcggcag gattgtgctt ttacgcagga
      301 taaaaagaga agattccggg agcaggctca atgaacgccc gcgctcgacg gcggaaaaaa
      361 gaaccacgcc tcccgattcg gttaaaccat attcagcata cacgacaccc actttagcct
      421 gctctgcctg cgagatattc tcggcacctt tcgccggatc ccaaacaacg gcattgcatt
      481 cctgctgcaa acgttcacta atccccaatt cctccagcct cgtgtcaccg ctaatcacga
      541 ccgactgatc tcccagctct ttacacagac gtattgcagc ttctgccgcc ttcgcctcgc
      601 tggtcagctc acagcgcgtc aacataacat cgctggcaaa ctgaataaac gcgtcacagc
      661 gctgctgttg gttaagttgg gtaagccgct cgttagcata gttgttaagc ggcgcatctt
      721 ctgcttgcgg ttcaagtcgc agcgggcgac ccagtgcctg agcaacgtta ttcaaaaatt
      781 cgctccgatt atccattctt tttctcctgc gcctgatgtt tcttaaacca actacggaaa
      841 ctctctccgt cagcttcagg aagatcgcgt gcttccatcc agtcgctaat cgcgccaaat
      901 ttgagtggtg ttttgccgcc attgataaac cagcttgccg catgagcgcc ggccatcatc
      961 ccgactttcc acaatcctgg atgactattg gcataagcga acatttttat cgcccgttgc
     1021 tctgcttttg cggtgatccc tttttcagcc atcacccgac gatgacgcaa aatcagtttt
     1081 gacagcggaa tacgcaccgg acacacgctg tcacaagcag tgcataaaga gcaggcgtag
     1141 ggtaaatctt taaaatcttt atagccgcca agtagcggag aaatcaccgc accaattggc
     1201 cctggataaa tagagccata tccatgaccg ccaatatggc gatatgccgg acaagtattc
     1261 atacaagccc cgcagcgaat acagcgcagc acatcccgaa attcagaggc cagcacctca
     1321 gaacgcccgt tatcgacaat aaccagatga aactcttcag gaccatcaac gtgccccgct
     1381 tcgcgcggtc ctgtcagcca ggtgttgtat cccgtcaaac gtgcaccaac ggcactgcgc
     1441 gccagcatgg tgatcaatac atctacctcg gcaaacgtgg gggcaatacg ctccattccc
     1501 atcacagcaa tatgcgtttt aggcagcgtg gtacacattc gcgcattacc ttcattggtc
     1561 accaggcata ccgaacctgt ctctgccacc gcgaaattac agccggtaat acctatttca
     1621 gcactgagga aatcttcgcg gattttttgc cggatgaata aggtcatcgc ttcaggcgtt
     1681 tccggcccct catagcccag acgttcgtgt agcactcgac ggatctgatg gcgatcttta
     1741 tgaattgccg ggaccacaac atgagatggc ggatcttgat ccagctggag aatatattca
     1801 cccagatcgg tttcaatcac ctgaatgcca gcatcctgca acacatgatt gacaccaatc
     1861 tcttcggtca ccatcgattt agatttcacc accttccggg catttttgcg ttgggcaacc
     1921 tgtaaaatgt agcgggtagc gtcttctttg gtttttgcaa accagacgtg accgccgttt
     1981 tgcgtcactt tttctgagag ctggtacaga taagcgtcga gattactcag aacatgatca
     2041 cgtatctggg cggcccgatc gcgccactcc tcccagtgcc ccaattcatc gaccattttt
     2101 tgccgatttg ccccaatacg ctgctgcgcg tttgccaccg ctttgcgcat gatcggatct
     2161 tcaatttgct gacggatgcg tgtcttaaaa tctgtattac tggttttgat cgacatcttt
     2221 atatcctcag cggctcatca acacttcagc aatatgcatc actttgactt tctgcccttc
     2281 ccgttgtaat cgcccactga tgttcagcag gcaactcacg tcagcaccaa ttaaatactc
     2341 agggcggact tccatcaggt gcacaacctt ttctttcacc atctcgccgg atatttcggc
     2401 cattttgacc gagaacgtgc cgccaaatcc gcagcaggta tcctgttcag caaaggtcaa
     2461 cagctccagt ccacgcacat ttttcagcag cgtaagtggc tcgtccttca ctcccagctt
     2521 acgggccagg ctacaagatg ggtgatacac agctctccct tgcaaactgg cacctacatc
     2581 gactacccct aatttattaa caataaaaga ggtgagatcc tgcatacgcg cggcaacctt
     2641 ttcggcacgt gatgcccatt caggttcatc cgccagatac gtcgggtaac tttttacggc
     2701 ataggtgcaa gagccagccg gtgaaataat gggatcgtcg ttatcctcca gtgcggcgat
     2761 cagatttttc atccctggaa tcgcttcttt gatataaccg ctattgatcg caggctgacc
     2821 gcagcatccc tgtttctccg ggaaatttac gcgacagccg agtttttcca gtagcagcac
     2881 ggagtctcgt gccattcttg atttcagggc gtcaccaata caggtgacaa agaaattgac
     2941 attcacaact aatactccat tacttcatgc ccatttatgc ccgatggttc ccctccttcc
     3001 gggggatcaa tcatcaataa atatggcgtt cttttttaat tggctagcaa cataccaccc
     3061 ctcatttgtg tgacaaatga taatctttca tccaaacttc aaatgcaata actaatttta
     3121 tttctttcgg catcgctcac attttatggg gaaatcgacg ttcatttttg gcataagaaa
     3181 tccatttaaa aaggctacat cccttatacc tattcagagc tacttattca ggggttattg
     3241 agatatacac tgatggtgta caaaaaaatc accgacaata atcacgtatc aagtaatagt
     3301 cggggtttta tttaattgaa gcaatttagt ttgcattttt atttaaaaac tgagctagag
     3361 tccatcagaa aaaaatatat aaagcccaaa ccaccgttgg aaatagaaat agttgaaagg
     3421 ctcacaactc ccggaatgcg gattatcttt gccctgattt ctggcggttt tattcagggt
     3481 gcaagctgtc tgaccctttc ccgatattct cccggagtac aaccaaactc gcggacaaac
     3541 gccttgtgaa aagatgattc actggcatag cctactgact cagcgatcac cacaacaggg
     3601 agcatttccc gggaaaacat ctgggccgct atttgtagac gcaactttgt taatacagcc
     3661 agcggcgtgg ttccggaaac atcacggaaa agctgggcaa aacttgcccg agacatgtgg
     3721 gcgatgctgg ccagcgattc gacggtccag gcgtgtcctg gcatttccag catttgctgt
     3781 attaccgcac caagacgtgg atgcagaagc aaactgagaa tgtttttctc tgtattaacc
     3841 tgtgcaatcc actcacgcac cgcgagggta aagaatgtag cgcagatctg gctacacagt
     3901 gcatccactc ccggcatcgc cgatctggat tcctgttgta aaaacgggat cgcctccctc
     3961 agccagttat attccacgct gtggttaacc ggtgctaaaa acagcgtttc cggcaaagac
     4021 gttaaaaaat aacgcgccga atgttgcaac cgaagagtgc cgcagacaat acaggtcgat
     4081 tcattatcga catgactcag gcgatgagct gaattttgtg gtagcaggac cacatttccc
     4141 gggcgtaatg taaaaatctc tcccgtcggc atttccagct tcgccgctcc ttgcgttaac
     4201 gcatgccaac gaataaccga taattccccg gcaccatgtt gaagctgcca gtcacttcct
     4261 aacacgcaat tcttatcgat cgttccttgt ggagcgttaa gcatcaacaa acggctaagg
     4321 gcatccatat cagactcctg agtggttttt gattacaaac accaacaatc ttagtaaata
     4381 actaatctaa taccatcaaa tggttaatca tgatttttgt tttgtgattc tgattaagaa
     4441 aaagaaaata ccagacgata agacaaaata tcaagcgttg tgaagaaatg gtatttgctc
     4501 ttttgcgtct atagtcatga tgtcaaatga acgtgtttcg acaggaaatc atcatgaata
     4561 aatatcaggc agttattatt ggttttggca aggctggaaa aacattagcc gtcacgctgg
     4621 caaaagcagg ttggcgagtg gctctcatcg aacaatcaaa tgcaatgtat ggcgggacct
     4681 gtattaatat cggctgcatc ccaaccaaaa cattggttca tgacgcacag cagcacacag
     4741 attttgtccg tgccatacag cgtaaaaatg aagtggttaa ttttttacgt aataagaatt
     4801 ttcataatct tgcggatatg cccaatatcg acgtgatcga cggccaggcg gagtttatca
     4861 ataatcatag cctgcgtgtt catcggcctg agggaaatct ggaaattcat ggcgagaaaa
     4921 tttttattaa taccggtgca caaaccgtgg ttccgccaat tcctggaatt accaccacgc
     4981 ctggtgtata tgacagcacc ggattactta atctaaaaga attgcctggg catttaggta
     5041 ttttgggcgg cggatatatt ggcgttgagt tcgcctctat gttcgctaat tttggcagca
     5101 aggtaaccat tttagaagca gcttcgctgt ttttgcctcg ggaagatcgg gatattgctg
     5161 ataatatcgc gacgatttta cgcgatcagg gcgtcgatat tatcctcaat gcccatgtgg
     5221 agcgaatcag tcaccatgaa aatcaagtgc aagtgcatag cgagcacgcc caactggcgg
     5281 tggatgcact gttaatagct tccggtcgtc aaccggctac cgcttcgtta catccagaaa
     5341 atgccggtat cgcagtaaac gagcgcgggg caattgtcgt tgacaagcaa ttacatacca
     5401 ccgcagacaa tatttgggcg atgggagatg ttaccggcgg gctgcaattt acttatattt
     5461 cactggatga ttaccgcatt gtgcgtgatg agttgctggg agaaggcaga cgtagtactg
     5521 atgatcggaa aaatgtgcct tattccgtat tcatgacacc gccactgtcc agggttggta
     5581 tgacagaaga acaggccaga gagagtggtg ctgatattca ggtggtgaca ttgcctgtag
     5641 ctgcaattcc gcgtgccaga gtgatgaatg atactcgtgg ggtattaaaa gcgattgttg
     5701 ataataaaac ccaacgtata ttaggggcat cactgctgtg tgttgactcc cacgagatga
     5761 tcaatatagt gaaaatggtg atggatgccg ggctgcctta tagcatatta cgcgatcaga
     5821 tatttactca tccgtcgatg agcgaatcac tcaatgatct attttcatta gtcaaataaa
     5881 ctcaaaatca gacgccagaa caaatattct ggcgtctcag agaaaagaat cttattaatt
     5941 ctctgttact ctataacttc ttaatcactt cattgatggt attttatatg tttaaaaaat
     6001 ctgttttatt tgcaacacta ttatctggcg ttatggaatt ttccaccaat gcagatgata
     6061 aaataattct gaaacatatc agcgtctcgt cagtatcagc atcaccgaca gttctggagg
     6121 ataccattgc tgatatagcc agaaaatata atgcttcatc ctggaaagtc acatcgatgc
     6181 gaattgataa taattcaacc gcaacagcag tattgtataa ataaggatgt tcacaatgga
     6241 aaaatacctg cacctgttaa gtcggggaga taaaattggc ctgtcattga ttcgtctgag
     6301 tattgcgatt gtttttatgt ggattgggtt attaaagttt gtcccttacg aggcagacag
     6361 cattacacca ttcgtcgcaa acagtccact aatgtcgttc ttttatgaac acccggaaga
     6421 ctataaacag tatctgactc acgaaggcga atacaaacca gaagcaaggg catggcaatc
     6481 ggccaataat acctatggtt tttccaacgg tcttggcgtc gtggaggtga ttattgctct
     6541 gctggttttg gctaatcctg tcaatcgctg gttaggttta ttgggaggac tgatggcata
     6601 taccacgccg ctggtaacac tctcattttt aatcaccacc ccggaggcat gggtacccgc
     6661 attgggtgac gctcatcatg gtttccctta tttatccggt gctggtcgct tggtattgaa
     6721 agatactctg atgctggcag gtgctgtcat gataatggca gattcggcgc gggaaattct
     6781 taaacaacgc agtaatgaat ccagttcaac gttaaaaact gaatattgat aatccgaaca
     6841 ctgtctgttg ttcacttttt caggcgtcag gccccctttc tgatgatgaa gcctgacgcc
     6901 atattaccgg agatatgaca gagtgaagat caatatcact cccctgttaa aagattcttg
     6961 tcgtctcagg taaaatatat gattaatatc aatcatgtat ttttacagca agacaaccac
     7021 gaacaaattc aggattaaaa tgttttaccg actccccaac ataacccaaa tcaagcgaac
     7081 taatttgtgc catttctttc tctgtcagtg agaaatccca gatagcaaaa ttttcttcga
     7141 tacgctgttg atgtgtcgat ttcggaataa cgaccactcc ccgttgaata ttccaacgca
     7201 gaatgacttg agaaatcgat ttttgatggg catctgcaat actctgaagc agattatttt
     7261 caaagggttt atgtcgtcca ccacctaacg gagcccatgc ttcaggctgc acgttataat
     7321 acttcatggt tgccagcgcc tctggttggg caaaataggg atgcaactcg acctggttga
     7381 ccatcggttt aactcttacc gtttcacaaa agttcgccag aacatgagga tagaaattgg
     7441 aaaccccaat tgcttttaat ttgccctctt cataggcatc ttcgagtgca cgccacacac
     7501 tgaaataatc gcgcatagcc tggtgcaata aatagaggtc gaagtactct agtcccgatt
     7561 tttttaatga tgcttcaata cctgctgctg ccgtatcctg attcaacata tcctgccccc
     7621 acaatttaga cgttataaat aactcatctc gggtacataa accttcagat atagcttcac
     7681 gaacggcttc accgacggca tcttcattac cgtataccgc tgcagtgtcg atgagtcgat
     7741 aacccgtgcg gatagcgttc aacactgact gtttgcacac gtttttatcg gtaacctgaa
     7801 aaacaccaaa tcccatcatc ggcattttca gattgttact taatacggaa aattccactg
     7861 tcagtccctc cattaggcat gaacaatgag tctacgttaa aacataacct caaattagta
     7921 tgtggatttt gatatcactt atgcaaaaaa ttcattaata atgtaggact gaaacctctc
     7981 tattttcggg gacaacgaag cagacgctac cagtgctttt gccttcgacc ttgctgtttt
     8041 tgatacactt agggccttgg gcataacgaa aatgtgcgat atcacgaaga attaatggag
     8101 aatg
//
LOCUS       DAJVQG010000163         8096 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJVQG010000163 DAJVQG010000000
VERSION     DAJVQG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8096
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.163"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            300..1571
                     /locus_tag="OQM83_003447"
     CDS             300..1571
                     /locus_tag="OQM83_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCS1521627.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(1601..2605)
                     /gene="dppF"
                     /locus_tag="OQM83_003448"
     CDS             complement(1601..2605)
                     /gene="dppF"
                     /locus_tag="OQM83_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCS1521628.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(2602..3585)
                     /gene="dppD"
                     /locus_tag="OQM83_003449"
     CDS             complement(2602..3585)
                     /gene="dppD"
                     /locus_tag="OQM83_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCS1521629.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(3596..4498)
                     /gene="dppC"
                     /locus_tag="OQM83_003450"
     CDS             complement(3596..4498)
                     /gene="dppC"
                     /locus_tag="OQM83_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCS1521630.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(4508..5527)
                     /gene="dppB"
                     /locus_tag="OQM83_003451"
     CDS             complement(4508..5527)
                     /gene="dppB"
                     /locus_tag="OQM83_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCS1521631.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(5835..7442)
                     /gene="dppA"
                     /locus_tag="OQM83_003452"
     CDS             complement(5835..7442)
                     /gene="dppA"
                     /locus_tag="OQM83_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCS1521632.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(7794..>8096)
                     /locus_tag="OQM83_003453"
     CDS             complement(7794..>8096)
                     /locus_tag="OQM83_003453"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521633.1"
                     /translation="VGLIRRASVASGNGTAFIVGCGATALFDQHTTHFPANFAPHISL
                     SINGFVTETIVFFYICLNLFLRYLWLALAFSAVRFSVHGINRSDIHLFLRFFSLNA"
BASE COUNT         1869 a         2189 c         2049 g         1989 t
ORIGIN      
        1 tttcggcttg ccgctgattt tacgattgct ttttactgtc agcacccggc aatcttcctc
       61 attgcgcgat gccttccagc ttaatttctt tatttgcaat agttgcagtt catatccggg
      121 ctgactccag actggtagtg aaatgcgcaa ttttgtagaa ttgcgcctcc tcgcgacctc
      181 cccagtgaat ctgttgcctc attttttaca tactgcgtat tcgacttctc cacctgttgc
      241 gcaagagaaa ctgggtttat tcatttttgc gaggccgact tctttctgga caggacttta
      301 tgcagcacaa cacactatcg aaacacaatc agaaattgcc gtttacacgc tacgacttcg
      361 gctgggtttt attatgcata ggcatggcga ttggtgccgg aaccgtgctg atgccagtac
      421 aaattggctt gaagggaatt tgggtattta ttaccgcagc gatcattgct tatcctgcca
      481 cctgggtagt gcaggacatt tatttaaaaa ccctttctga aagcgattcc tgtaatgact
      541 acaccgatat tatcagtcat tacctgggga agaactgggg aattttcctc ggggttatct
      601 actttttgat gattatccac gggattttta tctactctct ctccgtggtt ttcgacagcg
      661 cctcgtacct gaaaaccttc ggtttaaccg atgccgatct ttcacaatct ctactttata
      721 aagtcgctat tttcgccgta ctggtagcga ttgcgtctgg tggtgaacga ttactgttta
      781 agatttccgg gccaatggtg gtggtcaaag tagggattat tgtcgtgttc ggttttgcga
      841 tgatcccgca ctggaatttc gccaatataa ccgccttccc gcaagcctcc gtctttttcc
      901 gcgatgtctt gcttaccatt ccattttgct tcttttctgc agtatttatt caggtactta
      961 acccaatgaa tattgcctat cgtaaacggg aagcggataa agtactggca acccggctcg
     1021 cgctgcgtac ccaccgaatt agttatatca cgctcatcgc ggtgatcctg ttttttgcct
     1081 tttcgtttac cttctcaatt agccacgaag aagccgtttc tgcctttgaa caaaatatct
     1141 cagcactggc gctggccgcg caggtgatcc ctgggcatat cattcatatc acctctacgg
     1201 tgcttaatat ctttgccgta ctgaccgcat tctttggcat ttatctcggt ttccacgagg
     1261 ccattaaagg cattattctc aatctgttaa gccgaattat tgataccaag aaaattaact
     1321 cacgcgtgct gactctggcg atctgcgctt ttatcgtcat tacgttgacg atttgggttt
     1381 cgtttcgtgt atcggtgctg gtgttctttc agttgggaag cccgttatat ggtattgtgt
     1441 cgtgcctcat tccgtttttc ctgatctata aagtcgcaca actggaaaaa cttcgcggat
     1501 ttaaagcctg gctgattctg ctgtacggca ttttgctatg cttgtcgcca ctgttgaagc
     1561 tgattgagta aaccggagcg catggccccg gttttgtgag ttaacgctgc ggattttcat
     1621 cctgatcaac agcaaaacaa gctaccagtt gaccgccgta gtcttttagc tgcggctgca
     1681 actgggtgca ggggccgaag cgccgacgac agcgggcgtt gaaggcgcaa cccggcggtg
     1741 gattcagtgg gcttggtagt tcaccgctga gcttgatgcg ctcgcggcga tcgtccgggt
     1801 tcaggcgcgg cgtcgcggaa agtagcgcct gagtgtacgg atggcgcggg ttattgaaga
     1861 tttggtcttt cgttcccttc tccacgcagc ggcccaggta catcaccatc acttcatcag
     1921 caatgtgctc caccaccgac aggtcgtggg agataaagac ataagacagc cccaactcct
     1981 gctgcaaatc catcatcaga ttcagcacct gcgcgcgcac tgaaacatcc agcgcggaaa
     2041 ccggttcatc ggcaatcacc acatccgggt cgagcatcag accacgggcg atggcgatac
     2101 gctgacgctg accgccggag aacatatgcg gatagcggtc atagtgctcg gttttcaggc
     2161 cgactttcgc catcatcgac agggcttttt cccgacgctg ttctttgctt aagctggtgt
     2221 tgatcagcag cggctcttca agaatttgcc cgactttttt acgcggattc agcgaaccgt
     2281 aagggttctg gaagacgatc tggattttct gccgacgcag cttctgcgcc tgcggatcgt
     2341 gcttaagcag atcctgcccc tggtaataca gctcgccacc ggtgggcgtt tcaatcatcg
     2401 tcagcaaccg accgagggtc gatttaccgc agccagattc gcccactact gccagcgttt
     2461 tgccacgttc aaggttaaac gaaacgccat ccagcgcttt aaccagacgt tccggcgcga
     2521 acatgccttt cttcaccgga taatgttttt tcaggtcgat agcctgcaac agcggttgtt
     2581 gcagggtggc ctcttgcgta ctcatagtgt cggcctcccg gcatcatcaa gtgggtaatg
     2641 gcatttggac tggcgcccgt cagcgagcat attcagcgcc ggttcttcag cgcgacatct
     2701 gtcagtggca taggggcagc gcgggttaag caggcagccg ttcgggcggt cgtacttgcc
     2761 gggaacgaca cctggcaacg acgccagacg ttctttgtcc tgagcaaatt ctggcagcgc
     2821 acgcagcaat gcctgagtat acgggtgacg cggcgcatgg aagatggcgt gcgcatcgcc
     2881 cgtttccacc acctggcctg catacatcac aatgatttta tgcgcagctt ccgccaccag
     2941 cgccagatca tgggtaatta acaccagcgc catgttctct ttctgctgta gctccagcaa
     3001 cagttcgatg atttgcgcct gaatggtcac gtccagcgca gtggtcggct catcggcaat
     3061 cagcagtttt ggccgacagg caatcgccat ggcgatcatc acgcgctggc tcatgccgcc
     3121 ggaaagctga tgcgggtaaa catccagacg cgatgccgga tcgggaatac cgacctgatt
     3181 cagcaggtca atcgcccgct gacggcgagt gctcttatta ccgccctgat gcaccttaat
     3241 cgcttccatg atctggaaac ccacggtgta gcacgggtta aggctggtca tcgggtcctg
     3301 gaagatcatc gccacttcgg cacccaccag gttgcggcgc tctttttctg atatacgctg
     3361 caaatcctga ccgttaaaca ccagcttttc cgccattacg cggcccggat aatcaatcag
     3421 ccccataatc gccagtgaac tgaccgactt accggagcca gactcaccca caatcccgac
     3481 cacttcgccc tgttttacgc tgtagctgat gcggtctacg gcgcggaacg gtgcgctttc
     3541 gtcgccgaaa tgcaccgata atttatctac atttaataac gccatctcga acctcttact
     3601 gcttcagttt gggatcgagc gcgtcacgca gaccgtcacc catcaggtta aatgccagca
     3661 ccgtcagcag gatcgccaga cccgggaagg tcacgaccca ccaggcgctt tgcgcgaact
     3721 gcaacacgtc ggagagcatg gtgccccact ctggtgttgg cggctgcgct cccatgccga
     3781 ggaagccaag ggcggccata tcgagaatgg cgttagagaa accgagcgac gcctgaacaa
     3841 tcagcggcgc caggcagttc gggaagatgt taataaacat ctgacgcatc gccccggcac
     3901 ctgccacgcg agacgcggta acgtaatcgc gattcacctc caccagcacg gcggcgcggg
     3961 ttaagcgcac atagtgcggc aaggcaacga aggtcagtgc cagcgcggcg ttaccaatcg
     4021 acgggccgaa aatcgccacc agcaccagtg ccaacagcag acttggcagc gccagcatga
     4081 tatcgaccac gcgcatgatg atgttatcga ccaggccgcc gaagtaaccg gcgatcaggc
     4141 cgagaataac gcccataatc agcgataaca ccaccaccag acagccaacc agcagcgaca
     4201 agcgcgcgcc gtacatcagc cgcgacagca catcacggcc tacgtcatcg gtgcccagca
     4261 agtgcgccat gctgccgcct tcctgccagg ctggcggggc gagcagcgca tcgcggaact
     4321 gatccgccgg gttataaggc gcaatccagt tggcaaagat cgtgataaac agcacgatga
     4381 cgatgtaaac cagcccgaca accgcgcctt tgttgcgttt aaaatagtgc cagaactcct
     4441 gtaacggggt catcggcacc ggtgcgctaa tcactttatt ttcagtaacc tgtgacatga
     4501 tgtcccctta cttcttatga cgaatacgcg ggttcaccac gccgtacagc agatcgacca
     4561 gcaggttgac gaggataatc atcgtcgcca ccagcaatac gccgccctgc actaccggat
     4621 agtcgcggcg ttgcagtgcg tcaatcaacc agcgtcccag accgggccac gagaagatgg
     4681 tttcggtcag aatcgccccc gccagcaatg ttcccacctg caggccgata acggtcacta
     4741 ccggcagcat cgcgttacgc agcgcatgga cgataatcac ccgcatgcgg gtcaaccctt
     4801 tggcgcgcgc ggtgcggatg taatcttcac ccagcacttc cagcatcgaa gagcgcgtca
     4861 tacgaacgat aaccgccagc gggatagtac ccagcacaat agccggtaag atcatatggg
     4921 caacggcgtc gataaagttg ccatcttcac cccagatggc ggtgtcgatc agcataaaac
     4981 cggttaacgg attggagtca tcgaggaaca ccatatcgct cacgcgaccg gagacgggcg
     5041 tcaggttcca gtgcaccgaa accagcatga tcagcatcat gccccaccag aagatcggca
     5101 tcgagtagcc ggtcagcgcc aggccaactg cagtgtgatc gaaaatggaa ccgcgtttaa
     5161 ccgcagccag cacaccaacc ggaataccga ctgccgtagc aaaaatcatc gcgcagacgc
     5221 caagttccag cgtggcctga aagcgcggca cgaactcttc ccatactgga attcggcttt
     5281 tcattgaaat gcctagatcg ccgtgcatta cgccccaaat gtaatggaga tactgctgcc
     5341 acatcggttt atctaagccg agttcagcca gcagctgcgc gtgacgctca ggggagatcc
     5401 cgcgttcgcc tgccatgatc atcaccggat cgcccgggat catgtggaca aaggcaaatg
     5461 tgagaagggt aataccgata aacgtgggga tgacgagtcc caaacgtcgg agaataaact
     5521 gcaacataac ccggattctc tatagtgacc cgcagcctgg aactgcgtgt ctgtattgct
     5581 cacaaatcaa ttcccgccct tatcggacag ggaatgaagt attgctgccg gatggcgctg
     5641 cacgcagagg cacatcgcca ccgggtatac agattgttga caaagtgcgc tttgtttatg
     5701 ccggatgcgg cgttaacgcc ttatccggcc tacaaaatcg tgcaaattca atacattgca
     5761 gaattttcgt aggcctgata agcggagcac atcaggcagt tttgcctttg ccatcagtct
     5821 tgtatggctt ttaattattc gatagagacg ttttcgaagt gatgtttgcc taatggatca
     5881 accacatagc ctttgacttc tttgcgtacc ggttcaaaca cggtggagtg agcgatgatc
     5941 agtgccggag tctgatcgtg catcaccacc tgcgcttgct tgtacagttc aacgcgtttg
     6001 ttatggtcgt cggtagcacg cgccggttga atcagatctt caaacggttt gtagcaccat
     6061 tttgagtagt tagaaccttg ttcagaggcg gcgcagctga acagggtggc gaagaagtta
     6121 tccgggtccc cgttatcgcc agtccagccc atcattaccg tctgatgttc gccatctttc
     6181 gcacgcttga ggtactcacc ccattcgtag gtgacgattt tggcctgcac gcccactttc
     6241 gcccagtctg cctgaatcat ctccgccatg cggcgagcgt tcgggttata cggacgttgt
     6301 accggcatcg cccacaggtc gatggagaaa cctttttcca gacccgcttc tttcagcaag
     6361 gctttcgctt tttcaggatc gtaggtgtaa tcctgaacgt cgtcgttata gccccacatg
     6421 gttggcggga tcaggttttt cgctgataca cccgcgccct gataaaccgc tttgatgatc
     6481 gcgtctttgt tcaccgcgta ggtcagtgcc tggcgaactt tcacgtcatc cagcggtttt
     6541 ttctgcacgt tataggagag gtagccgacg ttcagccccg gcatttccat caggttgatg
     6601 gatttatcct gcttcatgcg agcgatgtct gccgggttcg ggtacggcat cacctggcat
     6661 tcattcttct gcaatttcgc gtaacgcacg gaagcatcag gggtaataga gaaaaccagc
     6721 gtatcgatct gcggtttggt gccccagtag ccatcaaacg ctttgtagcg gatacgggaa
     6781 tctttttggt actgctgtaa ctggaacgga ccggttccga ttgggttgag gtccagtttt
     6841 tccggtgtac cggctttcat catcgcatca gcatattctt ttgacagaat agaggcgaag
     6901 tccattgcca gatcagcgag gaacggcgct tccgggcgag tcagtacgaa ctgaactgtg
     6961 ttgtcgtcca cctttttcac ttcactgatc agctccggca agcccatgcc ttcgaagtat
     7021 tcgtagctgc cgccagagac tttatggtac gggttttgcg cgtttttctg acggtcgaac
     7081 gagaacacca cgtcatcggc gttcagttca cgcgtcggtt tgaattcttt attgtcgtgc
     7141 cacttcacac ctttacgcag atggaaggta taggttttac cgtcttcgct gacttcccac
     7201 ttttcagcga ggcccgggat cacttcagtg gtgccgattt taaattcaac caggcggtta
     7261 taaagcggta cggaagaggc gtcataggtg gtgccggagg taaacagctg cgggttaaac
     7321 ccttccggag atccttctga gcaataaacc agagttttag cctgaacact tgctgcgacg
     7381 gtcatagcca ccaggctgag accaagcttc agcatccctg actttttcaa ggaaatacgc
     7441 attattctgc tccaattgtg atgtttgttg ttttaaccct ttgcagtggg ttgtcgctgc
     7501 ctgacctttt ttgttttttg cccggtcggg tcaacgttat gaggtgggga tgccgtacta
     7561 attaacatca gtgtaacagt gcggatcctc cataaaatgc ccctcgtgac ctacaatctg
     7621 tcaacagaat gtgaaaacgt caatacagcc aaccgggatt tacaccaacg gtgagaatcc
     7681 acaaacaaag attaaaaaaa cttcaaacag ctatttgcag cagagaaatt tgtgctactc
     7741 caacataacc agaacaatca gcttaatatt tagcaacatt tggtgattaa gttttatgca
     7801 tttaatgaaa aaaatctgag gaaaaggtgg atatctgagc gattaatacc atgaacgcta
     7861 aaacgcacag cggaaaatgc cagcgccagc cataagtaac gcaaaaaaag atttaagcaa
     7921 atataaaaaa agacaatggt ttctgtcaca aatccgttaa tggatagtga gatatggggc
     7981 gcaaagttgg caggaaaatg tgtggtgtgt tggtcgaata aggcggtcgc gccgcatccg
     8041 acaataaatg cggtgccgtt gcctgatgcg acgctggcgc gtcttatcag gcctac
//
LOCUS       DAJVQG010000164         8079 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJVQG010000164 DAJVQG010000000
VERSION     DAJVQG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8079
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.164"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..3018
                     /locus_tag="OQM83_003454"
     CDS             <1..3018
                     /locus_tag="OQM83_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCS1521634.1"
                     /translation="SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTE
                     IIDVKQCYPNTALVGVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDG
                     TLKPAYSNNMAWCLWDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTE
                     PRITCNAWLTTQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVM
                     PDDGAPFRYSFSALKDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAF
                     GCTSRGQAHRAGLWLIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGR
                     VLAVNSQTRTLTLDREITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDG
                     VAEYSVWGLKLPTLHQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQS
                     GTVNGVTPPAVQHLTAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLV
                     STARTTETTYRFTQLALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAALSRIELTPGY
                     FQITATPHLAVYDPTVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHD
                     YYFYIRSVNTVGKSAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAP
                     DLAEIRTSITDVSNEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQD
                     GRLYIAGIGAGIENTPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFL
                     KRLTAPTITSGGNPSAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGML
                     EATQVRGDFVKAVSKSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGI
                     AYDDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTL
                     EFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
     gene            3089..3605
                     /locus_tag="OQM83_003455"
                     /pseudo
     CDS             3089..3605
                     /locus_tag="OQM83_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            3670..5074
                     /locus_tag="OQM83_003456"
                     /pseudo
     CDS             3670..5074
                     /locus_tag="OQM83_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            5023..5175
                     /locus_tag="OQM83_003457"
                     /pseudo
     CDS             5023..5175
                     /locus_tag="OQM83_003457"
                     /inference="COORDINATES: protein motif:HMM:NF015371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            5152..5331
                     /locus_tag="OQM83_003458"
                     /pseudo
     CDS             5152..5331
                     /locus_tag="OQM83_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            6046..6753
                     /locus_tag="OQM83_003459"
                     /pseudo
     CDS             6046..6753
                     /locus_tag="OQM83_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            6753..7334
                     /locus_tag="OQM83_003460"
     CDS             6753..7334
                     /locus_tag="OQM83_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1521635.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPVAPIG"
BASE COUNT         2129 a         1964 c         2292 g         1694 t
ORIGIN      
        1 tcgcagtatc tggcctcggt ggtggtggat aacctgccgc cgcgcccgtt taatatccgg
       61 atgcgcagaa tgacgccgga cagcaccaca gaccagctgc agaacaaaac gctctggtcg
      121 tcatacaccg aaatcatcga tgtgaaacag tgctacccga acacggcact ggtcggcgta
      181 caggtggatt cggagcagtt cggcagccag caggtgagcc gtaattatca tcttcgcggg
      241 cgcattctgc aggtgccgtc gaactataat ccgcagacgc ggcaatacag cggtatctgg
      301 gacggaacgt taaaaccggc atacagcaac aacatggcct ggtgtctgtg ggatatgctg
      361 acccatccgc gctatggcat ggggaaacgt cttggtgcgg cagatgtgga taaatgggcg
      421 ctgtatgtca tcggccagta ctgtgaccag tcggtgccgg acggctttgg cagcacggag
      481 ccgcgcatca cctgtaatgc ctggctgacc acacagcgca aggcgtggga tgtgctcagt
      541 gatttctgct cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct gacgttcgtg
      601 caggaccgac agtcggataa ggtgtgggcc tataaccgca gtaatgtggt gatgccggat
      661 gatggcgcgc cgttccgcta cagtttcagc gccctgaaag accgccataa tgccgttgag
      721 gtgaactgga ttgacccgga taacggctgg gagacggcga cagagcttgt ggaggatacg
      781 caggccattg tccgttacgg tcgtaacgtc acgaagatgg atgcctttgg ctgtaccagc
      841 cgggggcagg cacaccgcgc cgggctgtgg ctgattaaaa cggaactgct ggagacgcag
      901 accgtggatt tcagcgtggg tgctgaaggg cttcgtcatg taccgggcga tgtcattgaa
      961 atctgcgatg atgactatgc cggtatccgc accggcgggc gcgtgctggc ggtgaacagc
     1021 cagacccgga cgctgacgct cgaccgtgaa atcacgctgc catcctccgg caccacgctg
     1081 ataagcctgg ttgacgggca ggggaatccg gtcagcgtgg aggtccagtc cgtcaccgac
     1141 ggcgtgaagg taaaagtgag ccgtgttcct gacggtgttg ctgaatacag cgtgtggggg
     1201 ctgaagctgc cgacgctgca ccagcgattg ttccgctgcg tgagtatccg tgagaacgac
     1261 gacggcacgt atgccatcac cgccgtgcag catgtgccgg aaaaagaagc catcgtggat
     1321 aacggggcgc actttgacgg cgaccagagc ggcacggtga atggtgtcac gccgccagcg
     1381 gtgcagcacc tgactgccga agtcaccgca gacagcgggg aatatcaggt gctggcgcgc
     1441 tgggacacgc cgaaggtggt gaagggcgtg agcttcctgc tccgtctgac cgtaacagcg
     1501 gatgacggca gtgagcggct ggtcagcacg gcccggacga cggaaaccac ttaccgcttc
     1561 acacaactgg cgctggggaa ctacaggctg acagtccggg cagtaaatgc gtgggggcag
     1621 cagggcgatc cggcgtcggt atcgttccgg attgccgcac cggcagcgct gtcgcggatt
     1681 gagctgacgc cgggctattt tcagataacc gccacgccgc atcttgcggt ttatgatccg
     1741 acggtacagt ttgagttctg gttctcggaa aagcggattg cggatatcag gcaggttgaa
     1801 accacagcac gctatcttgg cacggcgctg tactggatag ccgccagtat caatatcaaa
     1861 ccgggccatg attattactt ttatatccgc agtgtgaaca ccgttggcaa atcggcattc
     1921 gtggaggctg ttggtcagcc gagtgatgat gcatccggtt atctggattt tttcaaaggc
     1981 gagataggga aaacccatct ggctcaggag ctgtggacgc agattgataa cggtcagctt
     2041 gcgcctgacc tggctgaaat caggacgtcc attacggatg tcagcaatga aatcacgcag
     2101 accgtcaata agaaactgga agaccagagt gcggcaattc agcagataca gaaggttcag
     2161 gttgatacaa ataataacct gaacagcatg tgggctgtga agctgcagca gatgcaggac
     2221 ggacgccttt atatcgcggg tattggtgcc ggtattgaga acacccctga cggcatgcag
     2281 agtcaggtgc tgctggcggc ggacaggatt gcgatggtta atcctgcgaa tggcaacaca
     2341 aaaccgatgt ttgttggtca gggcgatcag atattcatga acgacgtgtt cctgaaacgc
     2401 ctgacggccc ccaccattac cagcggtgga aatccatcgg cattttccct gactccggac
     2461 ggaaagctga ccgctaaaaa tgcggatatc agtggcagtg tgaatgcgaa cgccgggacg
     2521 ctcaacaatg tcacggtaaa tgaaaactgt acgattaagg gcatgctgga ggcgactcag
     2581 gtcagaggtg acttcgttaa agctgtatcc aaatcatttc cgaaacaggc tggtacgtgg
     2641 ggtaacacgg aaacaccaaa cgggacggtt acagtcacca taagcgatga tcataacttt
     2701 gaccgccaga ttattattcc gcccattatt tttaacggta tagcgtatga cgatccgggg
     2761 agcggaaata acccaggagg cacgcgatac acgggttatg gttttgaagt tcgcaaaaac
     2821 ggcgtattaa tcgcatccag agaaactaaa ggtgccattc ccggtagtta cagtgcagtt
     2881 attgatatgc ctagtggtgg tggtagcgtc actctggagt ttaagatttt ccagaaaggc
     2941 aatcaggggg caggcaatat caccgactgt acggtgattg tgaccaaaaa agcggcttcc
     3001 ggcatcagta ttcgttgaaa tatttataac cccaataaat ggcgtcagga atgacgcctt
     3061 ttttattgca gaaaagcgag aggtaattat gcgtaaagtt tgtgcagcca ttttgtccgc
     3121 agccatttgc ctggccgtat ccggtgcgcc tgcatgggcg tctgaacatc agtccacgct
     3181 gagcgcgggg tatcttcatg cccggaccaa cgttcccggc agtgatgatc tgaacgggat
     3241 taacgtgaaa taccgttatg agtttacgga tacgctgggg ctggtgacgt cattcagcta
     3301 tgcaggagac aagaatcgcc agctgacccg ttacagcgat acccgctggc atgaagattc
     3361 cgtgcgtaac cgctggttca gcgtgatggc ggggccgtct gtgcgcgtga atgaatggtt
     3421 cagcgcgtat gcgatggcgg gtgtggctta cagccgtgtg tcgacttttt ccggggatta
     3481 tatccgcgta actgacaata aggggaaaac gcacgatgtg ctgaccggaa gtgatgacgg
     3541 tcgccacagc aacacgtctc tggcgcactg acggtttcat cgtgggtgtc ggttataaat
     3601 tctgattagc caggtaacac agtgttatga cagcccgccg gttcaggcgg gcttttttgt
     3661 ggggtgaata tggcagtaaa gatttcaggt gtactgaaag acggcacagg aaaaccggta
     3721 cagaactgca caatccagct gaaagcaaaa cgtaacagca ccacggtggt ggtgaacacg
     3781 ctggcctcag aaaatccgga tgaagccggg cgttacagca tggacgttga gtacggtcag
     3841 tacagcgtta ttctgttggt ggaaggattc ccgccgtcac atgccgggac catcaccgtg
     3901 tatgaagatt cccgacccgg tacgctgaat gattttctcg gtgccatgac ggaggatgat
     3961 gcccgtccgg aggcactgcg ccgttttgag ctgatggtgg aagaggtggc gcgtaacgcg
     4021 tccgcggtgg cacagaacac ggcagccgcg aagaaatcag ccagcgatgc cagcacatca
     4081 gcccgtgagg cggcaaccca tgcgactgat gctgcagatt cagcacgtgc agccagcacg
     4141 tcagccggac aggccgcttc gtcggctcag tcagcgtctt ccagcgcagg aacggcatca
     4201 acaaaggcca ctgaagcctc aaaaagtgct gccgctgcag agtcctcaaa aagcgcggcg
     4261 gctaccagtg ccggtgcggc gaaaacgtta gaaacgaatg cggcagcgtc acaacaatca
     4321 gcagccactt ctgcatccac cgcgaccacg aaagcgtcag aagctgccac ctcagcccgg
     4381 gatgcgtcgg cttcaaaaga ggcggcaaaa tcatcagaaa cgaacgcatc atcaagcgcc
     4441 agtagcgcag cttcctcggc gacggcggct gcaaattctg cgaaggcggc caaaacgtcc
     4501 gagacgaacg ccaggtcttc tgaaacggca gcgggacaga gtgcctcagc tgcggcaggc
     4561 tcaaaaacag cggctgcgtc gtctgccagc gccgcgtcaa caagtgccgg gcaggcctca
     4621 gccagtgcca ccgccgccgg aaaatcggca gaaagcgccg catcatccgc ttcaacagcc
     4681 acaacgaagg ctggcgaagc cactgagcag gccacggcag cagcgaggtc agcttccgca
     4741 gcgaagacat ccgaaacgaa cgcgaaagcg tcggaaaccc gtgcagaatc ctcaaaaacg
     4801 gctgccgcat cgtccgccag ttcggcggcg tcatcggcat catcggcgtc tgcttcaaaa
     4861 gatgaggcga ccagacaggc gtcagcagcg aagggcagcg ccacgacggc atccacgaag
     4921 gcgacagagg ctgctggtag tgcgacggca gcagctcaga gcaaaagtac ggcggaatct
     4981 gcagcaacgc gcgctgagac agcggcaaaa cgggcagagg atattgcatc cgccgtggcg
     5041 cttgaggatg cgagcacgac gaaaaagggg atagtacagc tcagtagtgc gactaacagc
     5101 acttccgagt cactggcggc aacaccaaaa gccgttaagg ccgcgtatga cctggctaac
     5161 gggaaataca ccgcacagga tgcaacgaca gcacagaaag ggataatcca gctaagcagc
     5221 gcgaccaaca gcacgtctga aacgctggcg gcaacgccaa aggcagtaaa agcagccaat
     5281 gacaatgctg agaaacgtct gcagaaagat cagaacggtg cggatatccc tggcaaagac
     5341 acctttacga aaaatattgg tgcctgccgt gccttcggtg ggtcagtaag cacaacaaca
     5401 ggaaactgga cgactgcaca gtttatcgag tggctggatt ctcagggagc atttaaccat
     5461 ccatactgga tgtgcaaggg ttcctggtct tatggcaata ataaaatcat tactgatact
     5521 ggctgcggta atattcatct cgccggagct gtcattgaag taatggggat aaagtcagcg
     5581 atgacgatcc gcattaccac tccgaccaca tccagcggtg gcggaacaac caacgcccag
     5641 tttacctata ttaatcacgg aacagattat tcacctggct ggcgaaggga ctataactcc
     5701 agaaataagc caacggcatc agagatcggg gcgctaccgt caggtggaac agcagtatca
     5761 tcagttaatc tggcttcaaa aggtcgggta gccgcgctga cagacaatac gcagggggca
     5821 gcaggtcttg agttatacga ggtgtataac aacggatatc caacagcgta tggaaatatc
     5881 attcacctga aagggatgac agccgttggc gaaggtgagt tactcatcgg ctggagtggt
     5941 acaagcggtg ctcatgctcc ggcatttatt cgttcacgac gggatacgac cgacgcaaac
     6001 tggtcgccgt gggcgcagct ttacacctcg gctcatcctc ctgcagagtt ttatccagtc
     6061 ggtgcaccaa tcccgtggcc atcagatacc gttccgtctg gttatgccct gatgcagggg
     6121 cagacttttg acaaatctgc ttacccgaaa cttgcagccg cttatccgtc aggcgtgatc
     6181 cctgatatgc gtggctggac gattaagggc aagcccgcca gtggtcgtgc cgtattgtct
     6241 caggaacagg atggcattaa atcgcacacc cacagcgcca gcgcatccag tacggatttg
     6301 gggacgaaaa ccacatcgtc gtttgattac ggcactaaat ccacgaataa caccggggcg
     6361 catacgcaca gtctgagcgg ctctacaagt tcagctgggg cccaccaaca tagtcagact
     6421 gggcccagaa cgaatagcgg gagtcaacct actggaatgt ttccggcagg gagtactcaa
     6481 gttagcggga ctaatcaggt tggtatcagt ggctcattaa ccagtggcac ctcccaatgg
     6541 gtaggtaaat catcttcaga aggaaaccat acacactcac tgtccggcac agcagcatct
     6601 gcgggtgcac acgcgcatac tgtcggtatt ggtgctcata cgcactctgt tgcgattggt
     6661 tcacatggac acaccatcac cgttaacgct gctggtaacg cggaaaacac cgtcaaaaac
     6721 atcgcattta actatattgt gaggctcgca taatggcatt cagaatgagt gaacaagcac
     6781 ggaccataaa aatttataac ctgctggccg gaactaatga atttattggt gaaggtgacg
     6841 catatattcc gcctcataca ggtctgcctg caaacagtac cgatattgca ccgccagata
     6901 ttccggctgg tttcgtggct gttttcaaca gtgatgagtc atcgtggcat ctcgttgaag
     6961 atcatcgggg taaaacggtt tatgacgtgg cttccggcga cgcgttattt atttctgaac
     7021 tcggctcatt accggaaaat gtcacctggt tatccccgga aggggagttt cagaagtgga
     7081 acggcacagc ctgggtgaag gatacggaag cagaaaaact gttccggatc cgggaggcgg
     7141 aagaaacaaa aaacagcctg atgcaggtag ccagtgagca tatagcgccg cttcaggatg
     7201 ctgtggatct ggagatcgca acggagaaag aaaccttgtt gctggaagct tggaaaaagt
     7261 atcgggtgtt gctgaaccgt gttgatacat caactgcacc ggatattgaa tggccagtag
     7321 cacctatagg gtaaattcgt aatgattacc taaatacgtt attcttttgt taaaaaagtg
     7381 attttgctat aggtagggag tatgttcttg gattaagaat aaaaaatcgc catgaaagag
     7441 cttcatggcg agtacataaa cgtacaaaat gtgatgaaag tagagtggcg taagccacta
     7501 tattaagata tgacaatagc gaagaaaagt aaataacatt ttatggacgt ttaattgtta
     7561 attataaaaa ctagagtgcc cgatattgtt tatcttgctt taaaataatg tactcaaacc
     7621 aaatttttga gctaaaagct caaataatat aaatgcaata ataatgttta ataagacaat
     7681 tagcttgctt tgtattttca tttcggaata ctgttaagta actttgatga ccctaacaat
     7741 agatcggaat tttcttagag tcaatggtca ctgtttttgc ttgcaaagca aatgttttgg
     7801 tggttataag tcgtcttaat atagtcacgc atgcaacttg ttccatgcgg agattaaaga
     7861 ttggaatata acgcgaagat ggaattaaat tacatttgca ataattaatt tattattact
     7921 caataattac cgatattatt attgtaaatt aatcattaga atgcaaaaag aaatgtaacg
     7981 gcaaaataag aggacgatag ctgagaaaaa agcgtgcttt cccaatctgg ggcaaatcac
     8041 gtctgaatga cacctgaaaa caggtggtag cctcaagaa
//
LOCUS       DAJVQG010000165         7989 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJVQG010000165 DAJVQG010000000
VERSION     DAJVQG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7989
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.165"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(318..1349)
                     /gene="rsmC"
                     /locus_tag="OQM83_003461"
     CDS             complement(318..1349)
                     /gene="rsmC"
                     /locus_tag="OQM83_003461"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCS1521636.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDMLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1452..1865
                     /gene="holD"
                     /locus_tag="OQM83_003462"
     CDS             1452..1865
                     /gene="holD"
                     /locus_tag="OQM83_003462"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCS1521637.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1834..2280
                     /gene="rimI"
                     /locus_tag="OQM83_003463"
     CDS             1834..2280
                     /gene="rimI"
                     /locus_tag="OQM83_003463"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCS1521638.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2295..2972
                     /gene="yjjG"
                     /locus_tag="OQM83_003464"
     CDS             2295..2972
                     /gene="yjjG"
                     /locus_tag="OQM83_003464"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCS1521639.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3063..4652
                     /gene="prfC"
                     /locus_tag="OQM83_003465"
     CDS             3063..4652
                     /gene="prfC"
                     /locus_tag="OQM83_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCS1521640.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            5045..5650
                     /gene="osmY"
                     /locus_tag="OQM83_003466"
     CDS             5045..5650
                     /gene="osmY"
                     /locus_tag="OQM83_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCS1521641.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5777..5938
                     /locus_tag="OQM83_003467"
     CDS             5777..5938
                     /locus_tag="OQM83_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCS1521642.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6060..7133
                     /locus_tag="OQM83_003468"
     CDS             6060..7133
                     /locus_tag="OQM83_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCS1521643.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            7130..7912
                     /locus_tag="OQM83_003469"
     CDS             7130..7912
                     /locus_tag="OQM83_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1521644.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT         1910 a         2066 c         2161 g         1852 t
ORIGIN      
        1 aggacactaa cacctgaagc tagcgcgtct accaattccg ccaccttcgc ataccatcaa
       61 ttcttaaaaa gaattgctac cacggaggcg cattctagtg gttttcagct tttcgtcaat
      121 agttaattat cgccggaggt gtaattgctg gaaaaatgtc catcaggaaa ctagcgtgca
      181 ggtttggtag gcatgcgggg gcagatgcca gatgcgacgc tggcgcgtct tatctggcct
      241 acgaagggct aacgtgcagg ttttgtaggt cggataaggc gttcacgccg catccgacac
      301 ggtattcggc gagataatta acctttcttc gcctggcggg tcataatggc gcgatacacc
      361 ttgaaacgcc cggtttgcgc aatcacttca tggaagccaa atgtctcatc cagcacgtcc
      421 gggtaaggca ggaaggcgtt cgctacaatt cgcagctcgc cgccgctatt aagatgacgc
      481 accgcgccgc gaatcagcgt ttgcgccgca tccaggctgg tttgcatccc atcgtggaac
      541 ggcgggttgg agatgatcat atcaaaacga cctttcacct cggaaaagac gttgctggca
      601 aagacttcac cttcaacaca gttggccgca agtgttgcgc ggctggcttc taccgctggc
      661 gcagagacat cgcacaaggt gagacgaatt ttcggcgaat ggcgcgcaaa ggcaactgaa
      721 agcacccccg cgccacagcc gacatccagc actttacctt tcgtgtgcgg agttaacgtc
      781 gagagcagca actggctacc gacatccaga ccgtcgcggc taaacacgcc aggcagcgtt
      841 ttgaccgtca ggccatcgac gctgtattcg ccccagaatt tatcggcatc aaataccggc
      901 tgtttttcca gacgaccaaa atagaggcca cagcgacgag cgctgtcgac tttattcaac
      961 ggcgcataat ctgccagcat ctgctcggcg ctgcgcacgc cgctgcggtt ctcgccaacg
     1021 acaaaaatat ctgtccccac tggcagcaga gaaagtaaat tcatcaactg gaactgggct
     1081 tccggtttgt tcttcggcca gtagtaaatc agcatatcgc aatctgcgac gtcatccacc
     1141 gtggcgacca gactgaaacg ggcgttatcc cccatctggc ggcttaatac ctgccagtgg
     1201 tggaattgct gggtatgagc acggctggcc gcggtatcta aacgcgcggg caggtcatcc
     1261 tgtaagtctc cggcaaacag aatacggctt tgttcgaaat catcactgtg acgcagcaag
     1321 acttcacttg ccggggtaaa tgcagacatg gaatgctcct caattgatac tggcggcgat
     1381 tatagccata tgttggcgcg gtatcgacga atttgctata tttgcgcccc tgacaacagg
     1441 agcgattcgc tatgacatcc cgacgagact ggcagttaca gcaactgggc attacccagt
     1501 ggtcgctgcg tcgccctggc gcgttgcagg gggagattgc cattgcgatc ccggcacacg
     1561 tccgtctggt gatggtggca aacgatcttc ccgccctgac cgatccttta gtgagcgatg
     1621 ttctgcgcgc attaaccgtc agccccgatc aagtgctgca actgacgcca gaaaaaatcg
     1681 cgatgctgcc gcaaggcagt cgctgcaaca gctggcggtt gggtactgac gaaccgctat
     1741 cactggaagg cgctcaggtg gcatcaccgg cgctcaccga attacgggca aacccaacgg
     1801 cacgcgccgc gttatggcaa cagatttgca catatgaaca cgatttcttc cctcgaaacg
     1861 actgatttac cagcggctta ccacattgaa caacgcgccc acgcctttcc gtggagtgaa
     1921 aaaacctttg ccagcaacca gggcgagcgt tatctcaact ttcagttaac gcaaaacggc
     1981 aaaatggcgg cgtttgcgat tacgcaagtg gtgctggatg aagctacatt gttcaatatt
     2041 gcggtcgatc ctgactatca gcgtcaggga ttgggaaggg tgctgctgga acatctgatc
     2101 gacgaactgg aaaaacgcgg cgtggcgaca ctatggctgg aagtccgtgc ctcaaatgct
     2161 gccgccattg ccctgtacga aagtttaggc tttaacgagg cgacgattcg ccgcaattac
     2221 taccccacca cggacggtcg cgaagacgcc atcatcatgg cgttgccaat cagtatgtaa
     2281 gacaaggtgg aataatgaag tgggactgga ttttctttga tgccgatgaa acgctgttta
     2341 cctttgactc attcaccggc ctgcagcgga tgtttcttga ttacagcgtc acctttaccg
     2401 ctgaagattt tcaggactat caggccgtta acaagccact gtgggtggat tatcaaaacg
     2461 gcgcgatcac ttcattacag cttcagcacg ggcgttttga gagctgggcc gaacggctga
     2521 aagttgaagc aggcttgctt aacgatgcct ttattaatgc gatggcggaa atctgcacgc
     2581 cgctgccggg cgcggtttct ctgcttaacg ccattcgtgg caacgccaaa atcggcatca
     2641 tcaccaacgg ctttagcgcc ttgcagcaag tgcgtctgga acgcacgggc ctgcgtgatt
     2701 acttcgattt gctggtgatt tccgaagaag ttggcgttgc caaaccgaat aagaaaattt
     2761 tcgattatgc gctggaacag gcgggcaatc ctgaccgttc acgcgtgctg atggttggcg
     2821 acactgccga gtccgatatt ctcggtggca tcaacgccgg gcttgcgacc tgctggctga
     2881 atgcgcacaa tcgcgagcaa ccagaaggca tcgcgcccac ctggaccgtt tcttcgttgc
     2941 acgaactgga gcagctcctg tgtaaacact gattgcctcc cccccgttga tgggtaaaat
     3001 agccgcaatt tttcgttttc aacaagcgcg gcgcgatgcc gcttactcaa gaagaaagaa
     3061 ttatgacgtt gtctccttat ttgcaagagg tggcgaagcg ccgcactttt gccattattt
     3121 ctcacccgga tgccggtaag actaccatca ccgagaaggt gctgctgttc ggacaggcca
     3181 ttcagaccgc cggtacagta aaaggccgtg gttccaacca gcacgctaag tcggactgga
     3241 tggagatgga aaagcagcgt gggatctcca ttactacgtc tgtgatgcag tttccgtatc
     3301 acgattgcct ggttaacctg ctcgacaccc cggggcacga agacttctcg gaagatacct
     3361 atcgtaccct gacggcggtg gactgctgcc tgatggttat cgacgctgca aaaggtgttg
     3421 aagatcgtac ccgtaagctg atggaagtta cccgtctgcg cgacacgccg atcctcacct
     3481 ttatgaacaa acttgaccgt gatatccgcg acccgatgga actgctcgat gaagttgaga
     3541 acgagctgaa aatcggctgc gcaccgatca cctggccgat tggctgcggc aagctgttta
     3601 aaggcgttta ccacctttat aaagacgaaa cctatctcta tcagagcggt aaaggccaca
     3661 cgattcagga agtccgtatt gttaaagggc tgaataaccc ggatctcgac gctgcggttg
     3721 gtgaagatct ggcacagcag ctacgtgacg aactggaact ggtgaaaggc gcgtctaacg
     3781 agttcgacaa agaattgttc cttgcgggcg aaatcactcc ggtattcttc ggtactgcgc
     3841 tgggtaactt cggcgttgat catatgctgg atggcctggt ggagtgggct cctgcgccga
     3901 tgccgcgtca gactgatacc cgtaccgtag aggcgagcga agacaaattt accggcttcg
     3961 tatttaaaat tcaggccaac atggacccga aacaccgcga ccgcgtggcg tttatgcgcg
     4021 tagtatccgg taaatatgaa aaaggcatga agctgcgcca ggtgcgtact gcgaaagatg
     4081 tggttatctc cgacgcgctg acctttatgg cgggcgaccg ttcgcacgtt gaagaagcgt
     4141 atccgggcga tatcctcggc ctgcacaacc acggcactat tcagatcggc gacaccttta
     4201 cccagggtga gatgatgaag ttcaccggta ttccgaactt cgcaccagaa ctgttccgtc
     4261 gtatccgcct gaaagatccg ctgaagcaaa aacagctgct caaagggctg gtacagcttt
     4321 ccgaagaggg cgcggtgcag gtgttccgtc cgatctccaa caacgatttg atcgttggtg
     4381 ctgttggtgt gctgcagttt gatgtggtgg tatcgcgcct gaagagcgaa tacagcgttg
     4441 aagcagtata tgaatcagtc aacgttgcca ctgcccgctg ggtagaatgt gcggacgcga
     4501 agaaattcga agagttcaag cgtaagaacg aaagccaact ggcgcttgat ggcggcgata
     4561 acctcgctta catcgctacc agcatggtca acctgcgcct ggcgcaggaa cgttatcccg
     4621 acgttcagtt ccaccagacc cgcgagcatt aattcttgcc tccagggcgc ggtagccgct
     4681 gcgccctgtc aatttccctt ccttattagc cgcttacgga atgttcttaa aacattcact
     4741 tttgcttatg ttttcgctga tatcccgagc ggtttcaaaa ttgtgatcta tatttaacaa
     4801 agtaatgaca tttctgacgg cgttaaatac cgttcaatgc gtagatatca gtatctaaag
     4861 ccgtcgattg tcattctacc gatattaata actgattcag aggctgtaat ggtcgttatt
     4921 catcacccat cgcttttgtg atggcgacca ttgacttctg tagagggtga agtctctccc
     4981 tattcagcaa tgcaacctcg tgttgccagg ctcaaattac gagcaaacat acaggaataa
     5041 atcgatgact atgacaagac tgaagatttc gaaaactctg ctggctgtaa tgttgacctc
     5101 tgccgtcgcg accggctctg cctacgcgga aaacaacgca cagactacca atgaaagcgc
     5161 agggcaaaaa gtcgatagct ctatgaataa agtcggtaat ttcatggatg acagcgccat
     5221 caccgcgaaa gtgaaggcgg cgctggtgga tcatgacaac atcaagagca ccgatatctc
     5281 tgtcaaaacc gatcaaaaag tcgtgaccct gagcggtttc gttgaaagcc aggctcaggc
     5341 cgaagaggca gtgaaagtgg cgaaaggcgt tgaaggggtg acctctgtca gcgacaaact
     5401 gcacgttcgc gacgctaaag aaggctctgt gaagggctac gcgggtgaca ccgccaccac
     5461 cagtgaaatc aaagccaaac tgctggcgga cgatatcgtc ccttcccgtc atgtgaaagt
     5521 tgaaaccacc gacggcgtgg ttcagctctc cggtaccgtc gattcccagg cacagagcga
     5581 ccgtgctgaa agtatcgcca aagcggtaga tggtgtgaaa agcgttaaaa atgatctgaa
     5641 aactaagtaa ttcgtcgtaa ttcgtcctcc cgaactttgt cgggaggcgc gatgtgcacc
     5701 acactaaaaa tatcgcattg agtgattttc acgctcatat caagcggtga cattaactat
     5761 ggtaaaggag acgcttatgt ttcgttgggg catcatattt ctggttatcg cgttaatcgc
     5821 cgccgcactt gggtttggtg gtctggccgg taccgctgca ggcgcagcta aaattgtctt
     5881 tgtcgtcggg attattctgt tcctggtgag tttgttcatg ggccgaaaac gaccctagat
     5941 ttcagaccat taagaaaatt ttatccaaag ccagtccagc ggactggctt ttgcggtttt
     6001 agcgaataat aaattgcgtt actttgtcat tctttcacaa caaaaacagg aaggcagagg
     6061 tggggcagcg aatacctgta acgcttggta atattgcgcc gttgtcgcta aggccgttcc
     6121 agcctggacg aatagctctg gtgtgcgaag gcggcggaca gcgtggaatt ttcacggctg
     6181 gcgtgctgga tgagtttatg cgcgcgcagt ttaatccttt cgatctttat ctcggcacat
     6241 ctgccggggc gcagaacctc tcggcgttta tctgcaatca gcccggttac gcgcgcaaag
     6301 tcatcatgcg ctataccaca aaacgcgaat ttttcgatcc attgcgcttt gtccgtggag
     6361 gaaatcttat cgatctcgac tggctggtgg aggccaccgc aagccagatg ccgttgcaaa
     6421 tggataccgc cgcgcggttg tttgacagcg gcaaatcgtt ttatatgtgc gcctgtcgtc
     6481 aggatgacta cgcgcctaat tactttttac caaccaaaca aaactggctg gatgtgattc
     6541 gcgcctccag tgcgatacct ggcttttatc gtagcggagt gtcgctggaa ggcattaact
     6601 acctggatgg cgggatcagt gatgcgattc cggttaaaga ggcggcaagg cagggcgcta
     6661 aaacgttggt cgtcattcgc actgtgccgt cacaaatgta ctacacgccg cagtggttca
     6721 aacgcatgga acgctggctg ggtgacagta gcctgcagcc gctggtcaat ctggtgcagc
     6781 atcatgaaac cagctatcgt gacattcagc aatttattga gaaaccaccg ggcaagctgc
     6841 ggatattcga aatttatccg ccgaagccat tacatagtat cgcgcttggc agtcggattc
     6901 cggcgctgcg tgaagactat aaacttgggc gtttatgcgg tcgttatttc ctcgccacgg
     6961 ttggcaagct attaactgaa aaagcgccgc ttacccgcca tctggtgcca gtggtgacgc
     7021 cggaatcgat cgttattccg cctgcgccag tcgccaacga tacgctggtt gccgaagtga
     7081 gcgatgctcc gcaggcgaac gacccgacat ttaacaatga ggatctggct tgatttgccg
     7141 ttttatcgat acccactgcc attttgattt cccgccgttt agtggcgatg aagaggccag
     7201 cctgcaacgc gcggcacaag cgggcgtagg caagatcatt gttccggcga cagaggcgga
     7261 aaattttgcc cgcgtgcagg cgttagcgga aaaatatcaa ccgctgtatg ccgcattggg
     7321 gttgcatccg gggatgttgg aaaaacatag cgatgtgtct cttgatcagc tacagcaggc
     7381 gctggaaagg cgtccggcga aggtggtggc ggtgggggag atcggtctgg atctcttcgg
     7441 cgacgatccg caatttgaga ggcagcagtg gttactcgac gaacagctga aactggcgaa
     7501 acgctacgat ctgccggtga tccttcattc ccggcgtata cacgacaaac tggcgatgca
     7561 tcttaaacgt cacgatttac cgtgcactgg cgtggttcac ggtttttccg gcagcctgca
     7621 acaggccgag cggtttgtac agctgggcta caaaatcggc gtaggcggta ctatcaccta
     7681 tccacgcgcc agtaaaaccc gcgatgtcat cgcaaaatta ccgctggcat cgttgttgct
     7741 ggaaaccgac gcgccggata tgccgctcaa cggctttcag gggcagccta accgaccgga
     7801 acaggctgtg cgggtgtttg acgtgctttg cgagttgcgc ccggaaccag aagatgagat
     7861 tgccgaagtg ttgcttaata atacgtatgc gttgtttagc gttagtgggt agcctaccct
     7921 tcgtaggcag catccggcag tcggcgcata atgcctgatg cgacgcttaa cgcgtcttat
     7981 catgcctac
//
LOCUS       DAJVQG010000166         7942 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJVQG010000166 DAJVQG010000000
VERSION     DAJVQG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7942
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.166"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            109..441
                     /locus_tag="OQM83_003470"
     CDS             109..441
                     /locus_tag="OQM83_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCS1521645.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            588..1949
                     /gene="yegQ"
                     /locus_tag="OQM83_003471"
     CDS             588..1949
                     /gene="yegQ"
                     /locus_tag="OQM83_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCS1521646.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2279..2596)
                     /locus_tag="OQM83_003472"
     CDS             complement(2279..2596)
                     /locus_tag="OQM83_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521647.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            3002..3901
                     /gene="yegS"
                     /locus_tag="OQM83_003473"
     CDS             3002..3901
                     /gene="yegS"
                     /locus_tag="OQM83_003473"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCS1521648.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
     gene            complement(3983..4756)
                     /locus_tag="OQM83_003474"
     CDS             complement(3983..4756)
                     /locus_tag="OQM83_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1521649.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(4862..5902)
                     /gene="gatD"
                     /locus_tag="OQM83_003475"
     CDS             complement(4862..5902)
                     /gene="gatD"
                     /locus_tag="OQM83_003475"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCS1521650.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(5950..7305)
                     /locus_tag="OQM83_003476"
     CDS             complement(5950..7305)
                     /locus_tag="OQM83_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCS1521651.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            complement(7309..7593)
                     /gene="gatB"
                     /locus_tag="OQM83_003477"
     CDS             complement(7309..7593)
                     /gene="gatB"
                     /locus_tag="OQM83_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCS1521652.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(7624..>7942)
                     /gene="gatA"
                     /locus_tag="OQM83_003478"
     CDS             complement(7624..>7942)
                     /gene="gatA"
                     /locus_tag="OQM83_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCS1521653.1"
                     /translation="EFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADD
                     DNDVEVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLD
                     SDE"
BASE COUNT         2148 a         2036 c         1782 g         1976 t
ORIGIN      
        1 acattatctc tccgccctgg caggcatcag aaaatcgctc atactttaat cggtaaacag
       61 cacctttaga tgctgttttc gatacgcaat ttcaatcaag gagtcattat ggctggttgg
      121 tttgaactca gtaagagcag tgataatcag ttccggtttg tgctaaaagc gggcaatggt
      181 gagactatcc tcaccagcga gctgtataca tcaaaagcct ctgcggaaaa gggcatcgcg
      241 tcggtgcgta gcaacagccc gcaagaagaa cgctatgaga aaaaaacggc aagtaacggc
      301 aaattctatt tcaatctgaa agccgccaat catcaaatta tcggctccag ccagatgtac
      361 gccaccgcgc aatctcgtga aaccggaatt gcctccgtta aagccaatgg cacaagccag
      421 acggtgaaag acaatacgta attacgatgc cgggcgtggg gtgtcggcgt ccggcgcttt
      481 accagatccc cccacagcgc tacaatgccc gcccttaaag tgggggcact cccctaaccg
      541 cttcatcagg tgaagcggag ctgacctgtc atcagaacga gagaattatg tttaaaccgg
      601 aactcctttc cccggcggga acgctgaaaa atatgcgtta cgctttcgct tatggcgcag
      661 atgctgttta tgcgggccag ccgcgttact ccctgcgtgt gcgcaacaac gaattcaacc
      721 acgaaaatct tcagctcggc atcaatgaag cccacgcgct ggggaaaaag ttttatgtcg
      781 tggtcaacat tgcaccgcac aacgccaagc tgaaaacctt tatccgtgac ctgaaaccgg
      841 tggtggaaat ggggccggat gcgctgatta tgtccgatcc agggctgatt atgctggtgc
      901 gtgagcactt ccctgaaatg ccgatccacc tctcggtgca ggctaacgcc gtaaactggg
      961 cgacggtgaa attctggcag caaatgggcc tgacccgcgt gatcctctct cgcgagctgt
     1021 cgctggaaga gattgaagag atccgcaatc aggtgccgga tatggagatt gagatcttcg
     1081 ttcacggtgc gctgtgcatg gcctactccg gtcgctgcct gctctctggc tatatcaaca
     1141 agcgcgatcc gaaccagggt acctgcacca acgcctgccg ctgggagtac aacgtccagg
     1201 aagggaaaga agatgacgtt ggcaacatcg tacacaagta cgagccgatt ccggtgcaaa
     1261 atgttgagcc gacgctaggt atcggcgcgc caaccgacaa agtgtttatg atcgaagaag
     1321 cccagcgtcc gggcgagtat atgaccgcgt ttgaagatga gcacggcact tacatcatga
     1381 actcgaaaga tctgcgcgcc atcgcccatg tagaacgcct gaccaaaatg ggcgtgcatt
     1441 cgctgaaaat cgaaggccgt actaaatctt tctactattg cgcacgcacc gcacaggttt
     1501 atcgtaaagc tatcgatgat gccgctgcgg gcaaaccgtt cgataccagc ctgctggaaa
     1561 ctctggaagg tctggcgcat cgtggctata ccgaaggttt cctgcgtcgt catactcacg
     1621 acgattatca gaactacgaa tacggttatt cagtttctga ccgccagcag tttgttggtg
     1681 agtttaccgg tgagcgcaag ggggacctcg cagcggtagc ggtgaaaaat aaattctccg
     1741 ttggcgacag ccttgagctg atgacgccgc aaggcaacat taactttacc cttgagcaca
     1801 tggaaaacgc caaaggtgaa gcgatgccgg tcgcaccagg cgatggttat actgtgtggc
     1861 tcccggtccc gcaggatctt gagctaaatt acgcgctgct gatgcgtaat ttctccgggg
     1921 aaaccacgcg taacccacac ggtaagtgat taatttcgat tatttttccc ggatggaaaa
     1981 ttcttagaaa ccgatcacat acagctgcat ttattaaggt tatcatccgt ttcgctgaaa
     2041 aacataaccc ataaaatgct agctgtacca ggaaccacct ccttagcctg tgtaatctcc
     2101 cttacacggg cttatttttt atgcataagc cctatccctg gtcaccgtct tccattgacc
     2161 acatcgatag aatctccctt catagcacga tgcctttcac gtaacggcat cgtgctcgca
     2221 caggttccgg ctaagcacaa ccagaacgcg catgtttgat gcttaccaaa aaatattctc
     2281 actctccaca tttgaatgtc agacgagcga cgccatgtaa tcctgcacct tctgtcttca
     2341 ggtcaactat ctgcattttt ttgccctgag taacacagaa atgggctgca tcatttttta
     2401 ctatattttc tgcaccagat attcttcccc tagctaaaga agcttcggct tcggtgtagt
     2461 attggttatc gagtttacgc tgaatattac ttttatatgc aagaccaaat ttaccgatac
     2521 ttgtttcatc attatgcaca gcacaaccag atataagaaa aatactaatt aatgatatag
     2581 cagctatctt tttcatctca ccttccccca ttaaatacca acgacactct ctagtgttta
     2641 aatataataa tggcatgatt attataattg aataggatta taataaatgt tctgtacaac
     2701 atttcctaca taagtaggaa ttacggacat tgaggccctt cagggtaact ccatgggggc
     2761 tttaatatat tatattgaag atgccactgt ttagttgaat attaggtata tgctcttttt
     2821 tgaaatttat cggtggcagc cgttagtatt cgctgtcccc attgcaagct cctggtggta
     2881 accactgaat cctccatact tgaactgact ttttatcctc cgactttcat cctgttctga
     2941 ctccaccttt tgttttctgc tctacactat ctacagacca atcataaagg cacatacgat
     3001 catggcagaa tttcccgcca gcttactgat tcttaatggc aaaagtactg acaatctacc
     3061 cttgcgcgaa gcaattatgc tgttgcgtga ggaaggaatg acgatccatg tgcgggtcac
     3121 ctgggagaaa ggcgatgccg cacgatatgt agaggaggcc cggaagttgg gcgtcgcaac
     3181 ggtgattgcc ggtggtggcg atggcaccat taatgaagtt tctacggcgt tgattcagtg
     3241 tgagggggat ggcatacccg cgctgggaat tttgccatta ggaaccgcca atgattttgc
     3301 caccagtgta gggattcctg aggcactgga taaggcgctg aaactggcaa ttgccggtaa
     3361 cgccattgcg atagatatgg cgcaggtcaa caaacaaacc tgttttatta atatggcgac
     3421 aggcggattt gggacgcgta ttaccacaga aacgccggaa aaattaaaag ccgcgctggg
     3481 tggcgtctct tacatcattc atggcttaat gcgcatggat actctgcaac cggaccgttg
     3541 tgaaatccgc ggtgaaaact ttcactggca aggtgacgcc ctggtcattg gtattggtaa
     3601 cgggcgtcag gccggtggcg gtcagcaatt gtgtccgaac gcgttaatta acgatggctt
     3661 gctgcaactg cgcattttta ccggcgatga aattcttccg gctctcgtat caaccttaaa
     3721 atctgacgaa gataacccga atattatcga aggcgcttcg tcgtggtttg atatacaagc
     3781 cccacacgaa atcactttta atcttgatgg cgaaccgttg agcggacaaa actttcatat
     3841 tgaaatactt ccggcggcgt tgcgttgtcg attaccacca gattgtctat tattgcgtta
     3901 atcaaactat tctctcttga accagggaga atccgaaatg ccggatgcgg tgtaaatacc
     3961 ttatccggca tacagaacaa tactacaccg taattaattt tacccgtgtc ttctgcaatg
     4021 ccagtttatc cacctctgat acagcatcat cggtaataat aatgtcaatt ttttcgattg
     4081 gcagtacctg attaaaaccg cgacggttaa atttcgatga atcgagaacg gcaatcactt
     4141 tattagcggc ggtgaccatc gcaccgctaa tcgaataacc ttcattaaag gtcgtaatac
     4201 cattaaccgc atcaatacca tcagcaccga caaacattaa atcggcatta atatcctgca
     4261 atgaacgctc ggcaatggaa ccatgcatcg agcgcgtttt atggcggacc gtgccaccac
     4321 agacgaccag agtaatgtct ttattttcgg aaagggcaaa cgctgccggg agactgttgg
     4381 taatcaccgt gatattttta gcggtcatta atccttcggc gataagcatt gtcgtacttc
     4441 cgctgtcgag aataacggtc atcccttcat ggatcatttt gactgccgcc tgcgctattc
     4501 tgttttttgg cgcgctggcg agctgaaatc gctctttaaa cccgacttcc tgggtctcgg
     4561 tttcactatt accggacatt attttcgccg caccgccatg aaagcgcgta accacgcctt
     4621 tttgttcgag aaagcgcaaa tcggcacgga ttgtcgcttc cgaggcagca aatactcccg
     4681 ccagatcctg aacaagcacg gttccctgtt cattcactaa ttggatgatc ttattccttc
     4741 gctcgaatga attcatcgtc atttacccat attcaattgt ggctagtgta aacgaagggg
     4801 gaccattggt gaataatctg ttttgcacaa taccgggcca gcatcacgct ggcccgcggt
     4861 ttcatggaat gagcaacact ttgcccggca tggcattacg agcgatgtca cgcaccgcct
     4921 gggtgaagct ttcaaagctt ccacggtgag cgattaatgg ctccaggctt aacttacgtt
     4981 ctgtcagcaa ccggctcgcc gtttcccact cctgccccgg ccaagggctg gaatagttca
     5041 tccaactgcc gataaccgtc agctctttac gcaatatttt gccaaacgtt gccgatgtta
     5101 aatgcagatc ctgatgcaac gtgcccacca gcgccagttg ggcatgagga ccggcaatct
     5161 ctaccgccag ttcgacagtt tgcggtacgc cagccgtctc gaggataagc tgattaaagc
     5221 gcagttcgcg taaaacgctc tgcatttgcg gcgcgctcat ttcgctactg ttaaatgttt
     5281 gcatcgcacc gaaagatttt gccaatgcca gtttttctga gctaatgtcg atcgccgtca
     5341 cactctttgc tcccagcgcg acagcgcact gaatagccag caggccaatg gttccggcac
     5401 caataataat aacgttttta ttctcacaac cttgcgctaa atgaaaagca tgcaggccaa
     5461 cggtaatcgg ctcaataaaa gccccatcct caataggcat atccgtgggt agagcaaaga
     5521 cattttttcg cttaacgaca atatattcag caaatccacc atcacgccgc gagccaataa
     5581 aatcatattt tgcgcactgg gaataaaacc ctttcaaaca ctctggataa gtaaaacagg
     5641 gtaataacgg cacacaggca accgcatcgc cagggtgtaa atcatcaaca ccggatccca
     5701 ccgcatcaat atagccgcta aattcatggc ctaacgttat tggataataa tgtgcaccat
     5761 ttttaaatat cctgggtaaa tcggaaccac ataagcccga gctggcaatt tttacccgca
     5821 cctcatcctg atgtttaatt tcaggaatga cgctttctgc aacgcgcacg ataccatcag
     5881 tatcattcac cattgatttc ataaaaactc ctgattgtta agggggataa cctccccctc
     5941 aggtaaaaat tattctgcga gaacggtttt ctcttgttta ataaaaccac gcgctctacg
     6001 ccaggtcatg aaaataccgg taagataaat tgcgccgata ataatgaaac cgggaatatt
     6061 ttgcgtggag aaaacctgaa tcagtaacca ggtaatggga gaaccgccct gatccattga
     6121 agccaccata cccccggctt ttaacgcccc agcattagcc gccagttggg tgtgcaaacc
     6181 aatagtttgc gtcgcgatcc acagggtgat gctcataatg atgacacccg agattaaggt
     6241 gcggaacaga tttccacgat gcaccgcgac cgccatcgcc acgaagaagc cgatggtggc
     6301 aagatcgcca aacggcagta cctgattacc cggaacacaa acagcaatta aaatggtgag
     6361 cgggataaaa atcaggcttg ccgataccac cgccgtatgc cccagcagta atgcgggatc
     6421 caggccaatc aggaactcct gaccgccgaa cttcgcctgt aaacgactac gggcctgctt
     6481 agcgatgggc gttaaaccat ccatgatggg tttaatcacc cgtggcatca gcagcataac
     6541 tgccgccgtt tttaccgcca gctgcaatac acctttgaca tcgtaaccgg cgaggatgcc
     6601 gataatcagc cccatcacaa aacccacggt gacaggctcg ccaaatggac caaatttgcg
     6661 ctgaatatcg tcggcgctaa atttaattcg gttaacgcct gggattttct cgatgatagc
     6721 atcgaccagc accgcaatcg gccccatata cgccgacgta ccgtgcggaa tagcaatacc
     6781 ttccagctca aagaaatttc gggtatcgcg ggcaaaccag tcgccgagct tataaacaaa
     6841 cgccgcgtga attaccacac ctgccatccc tatcatccat gaaccggttg ccagatgcag
     6901 caacgcgccg gtgaaggtca tatgccagat attccagata tcaacattta ccacccgcgt
     6961 catacgggtc agtaacatcg ccacgttaac cagaatcgca atcggaatcg ccaccagcgc
     7021 aatttgcgac gcccaggtca ttggtgaaga gcccggccag ccgacatcga ccacatgcag
     7081 attcaggtcg aaattttccg ccatcgcttt cgccgccgga ccaatggaat ccagcattaa
     7141 gccaatcaca aggccaatgc caacaaagcc aatcccgata tgcagacccg ctttaaagca
     7201 atcgcctgcc ttcatgccta atattttaga aaaaataatg atgacaatcg gaagcatcac
     7261 cgtagggccg aggtcgagaa tataacgcat gacttctgaa aacataggtc acccctgtaa
     7321 gatagtcaga attttatttt gtaatgcttc gataccgaca ccagaaataa aaggcatgcc
     7381 gtgaactaac ggaatatcgc caaaactacg atccactttg gcagtggtgc atatcaaatg
     7441 cacaccatcc atataggttt ctatttcatt aacccgacac tggattaatt caacaggaat
     7501 actatgattc tgacacaact ctttaatttc ttccgccgcc atcgtagagg tcgcaaccgc
     7561 gcctccgcaa gcgacaataa tcttgcgttt cataaatagt cctttttatt gttacagagg
     7621 gatttattcg tctgaatcta aaacatactt tgtgaagtat tcctttaact gggtttcagg
     7681 aagagtgatt agtgtctcga cgatatcggg ctgttgtaac ttgccaaata aacagcgtaa
     7741 aagtttcaat tgctgctgcg gattctccac aatcaacgca ataaccaacg atacctccac
     7801 gtcgttatca tcatccgctt gctgaaaatg aactttattt gttggcctta acagataaat
     7861 ggctgacgac ttagcatgaa tcgcctcaca atgcggtatt gcaatggcgt gctgctcaag
     7921 cattatcccg gtagggaatt ct
//
LOCUS       DAJVQG010000167         7910 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJVQG010000167 DAJVQG010000000
VERSION     DAJVQG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7910
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.167"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            78..2393
                     /gene="bisC"
                     /locus_tag="OQM83_003479"
     CDS             78..2393
                     /gene="bisC"
                     /locus_tag="OQM83_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCS1521654.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFTRRSAVLSSMQGSLPGGCDAVDKIPVARI
                     VEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISEC
                     FWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAEL
                     SERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQLIEMPEN
                     PDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEPDEWQGN
                     AEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITEGDMVRV
                     WNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDLPSSRLG
                     NGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2362..2802)
                     /locus_tag="OQM83_003480"
     CDS             complement(2362..2802)
                     /locus_tag="OQM83_003480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1521655.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2799..3362)
                     /gene="tag"
                     /locus_tag="OQM83_003481"
     CDS             complement(2799..3362)
                     /gene="tag"
                     /locus_tag="OQM83_003481"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCS1521656.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3520..4218
                     /locus_tag="OQM83_003482"
     CDS             3520..4218
                     /locus_tag="OQM83_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1521657.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4447..5655
                     /locus_tag="OQM83_003483"
     CDS             4447..5655
                     /locus_tag="OQM83_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1521658.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFCVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5979..7670
                     /gene="eptB"
                     /locus_tag="OQM83_003484"
     CDS             5979..7670
                     /gene="eptB"
                     /locus_tag="OQM83_003484"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCS1521659.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDETYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7762..7838
                     /locus_tag="OQM83_003485"
     tRNA            7762..7838
                     /locus_tag="OQM83_003485"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7796..7798,aa:Pro,seq:cgg)
BASE COUNT         1814 a         1943 c         2099 g         2054 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttacgc ggcgttctgc ggtgctctct tccatgcagg
     1201 gcagcttgcc gggtggctgc gatgcggtgg ataaaatccc tgttgcccgc attgttgaag
     1261 cactggaaaa ccctggtggc gcatatcaac acaacggtat gaaccgacat ttcccggata
     1321 ttcgttttat ctggtgggcg ggcggtgcca actttactca tcatcaggat accaatcgtc
     1381 tgatccgtgc ctggcaaaaa ccggagctgg tggtgatctc tgaatgcttc tggacggcgg
     1441 cggcgaaaca cgcggatatc gttctgcctg cgactacctc ttttgagcgt aatgatctca
     1501 ccatgaccgg tgattacagt aatcagcatc tggtgccgat gaagcaagtg gtgccgccac
     1561 gctatgaagc gcgtaatgat tttgatgttt ttgccgagtt gagtgaacgt tgggagaagg
     1621 gcggctatgc gcggtttacg gaaggaaaaa gtcagctgca atggctggaa acgttttata
     1681 acgttgcccg gcagcgcggg gcaagccagc aggttgaatt gccgccattt gctgagttct
     1741 ggcaagccaa ccagttaatt gagatgccgg aaaacccgga cagcgagcgg tttattcgct
     1801 tcgctgattt ttgccgcgat ccgctggcgc atccgttaaa aaccgccagc ggcaagattg
     1861 aaatcttctc acagcgtatt gccgattacg gttacccgga ttgccctggg catccaatgt
     1921 ggctggagcc ggacgaatgg cagggcaatg ccgaaccaga acagttgcag gtactttctg
     1981 cccatccggc gcaccgcctg cacagccagc tgaattacag ttctctgcgc gaattgtacg
     2041 cggtagcaaa ccgtgagcct gtcaccattc atcctgacga tgctcaggcg cgcggcataa
     2101 ccgaagggga tatggtgcgg gtgtggaact cccgtgggca gatccttgcc ggagcggtca
     2161 ttagcgaggg aattaaacct ggcgtgattt gcattcacga aggggcatgg ccggatctgg
     2221 atttaaccgc tgacggtatt tgtaaaaacg gcgcagtgaa cgtgctgacc aaagatctcc
     2281 ccagctcgcg gctggggaat ggctgtgcgg gtaatacggc gctggcatgg ctggaaaaat
     2341 acaacggtcc ggaactgaca cttacagcgt ttgaaccacc ggccagctca taatccaggt
     2401 gggtagttgg gtttcatcct gccatgcgca atcgacaatg tgaaaaccct gcgcacggta
     2461 aaaatctatc gccggttgat ttttttgata aacctccagc atcagatggg gatagcgctg
     2521 ctgcacatac tgcatcagcg ccttaccaat accgcgtctg acggccttcg gtgcgacaaa
     2581 cattgctgcc agaaatcggc cttccataat gctgacaaaa ccgagaagct taccgtcctc
     2641 ttcccagacc cagttttgcg cgttggcaag ataggcatcc cgcaccagcg gaatgcagtc
     2701 acgccagtaa ttcgctttta taaagggatg cccccaggtt gtactttcca gccacagttc
     2761 gaggatcgcg gggagttctg aacgttgcgc ttcccgaatc atggtttatt tcccggatag
     2821 caacagcagc caaccacatg atcattcacc agcccacatg cctgcataaa ggagtaacag
     2881 attgtggtgc cgacaaactt aaaaccacgt tttttcagtg ctttagatag ggcgtcggag
     2941 gcggacgtag atgtgggaat ttcgctcaac gttgtggctt gtgtcacctg tggctgatga
     3001 tttacaaacg accagacaaa gtcggcaaac ggttcgccgt tctgttccat ttgcaggtac
     3061 gcccgcgcat taccaataat tgcctgaatt ttccctcgat ggcggataat cccggcgtcc
     3121 tgtaccagtc tttcgacatc ctcttcctgc attgctgcga ccttcaccgg atcgaactga
     3181 tgaaagcagg cgcgatagtt ttcgcgtttt ttgaggacgg tgatccacga taacccagcc
     3241 tgctgccctt caaggcagat catttcgaac agttttttac tgtcagtttc aggcacgccc
     3301 cattcattat catggtaggc aatataaagc gggtcctgac tcacccagcc gcaacgttcc
     3361 atactttccc tcgctatgta cccgatcgta aaaatttcag ccgcttttct tgacgcatcg
     3421 gtgaaaaagt caatatttaa tacagggcta ctatgcccga tatccttcat acaatgacaa
     3481 taatattgcc gaattcggct cttccctgga gtcgtgttaa tgatcataaa aaaaagcggt
     3541 ggtcgctggc agctaagcct gctggcgagc gtggtaatca gtgccttttt tctcaacaca
     3601 gcttacgcct ggcaacaaga atatatcgtt gatacgcaac ccggacattc cacagagcgt
     3661 tacacctggg atagtgatca tcaacctaat tacaacgata ttttgtcgca acgtattcaa
     3721 agtagccaaa gggcgctggg actggaagtc aatctggcgg aagaaactcc tgtggatgtg
     3781 accagcagta tgagtatggg ctggaatttt cctttatatg aacaggttac aaccggcccg
     3841 gtcgcggcat tacattacga tggcacaacc acctcgatgt ataacgagtt tggcgacagt
     3901 actaccacgc agaccgatcc gttatggcat gccagcgtga gtaccttagg ctggcgtgtt
     3961 gactcccggc ttggcgatct ccgaccctgg gcgcaaatca gctataacca gcaatttggc
     4021 gagaatatct ggaaggcgca atcaggcctg agccggatga cggcgacaaa ccagaacggc
     4081 aactggctgg atgtcaccgt aggcgctgat atgttgctca atcaaaatat tgccgcctat
     4141 gccgcgctaa ctcaggcaga aaataccact aataatagcg actatctgta tacgatgggg
     4201 gttagcgcca gattttaacg taacagtcac aattgaaacc attaaataac aataattgtg
     4261 gcgatagtgg gtgctaactt accaaataat aaatttggtg aataattgtc gcgtcattca
     4321 ttcctgaact aaggcatttc attccgttct gatggcattt catgccgttt ttccccaggc
     4381 ataaagtgca cttcgttatg gttgtcggca gagatttttc ctttttatta ctgcaggaat
     4441 actgccatga caccttcaaa ttatcagcgt acccgctggc tgacactcat cggtactatc
     4501 attacccagt ttgcgctggg gtcggtttat acctggagcc tgtttaatgg cgcgctttcc
     4561 gccaagctgg atgcgccggt aagccaggtc gctttctctt tcggcttgtt aagtctgggg
     4621 ctggcaattt cgtcttctgt tgcgggcaaa ttacaggaac gtttttgcgt taaacgcgtc
     4681 accatggctt ccggcatttt gctgggatta ggcttcttcc tgaccgcgca ttccaacaac
     4741 ctgatgatgc tgtggttaag cgccggtgtg ctggtcggtc tggcggatgg cgcgggttac
     4801 ctgctgacgc tctctaactg cgtgaagtgg ttcccggagc gtaaaggtct gatctccgcg
     4861 ttcgctatcg gttcttatgg tctgggtagc ctgggtttca aatttatcga cacgcagctg
     4921 ctcgaaacgg tgggtctgga aaaaacattt gtgatttggg gagcgattgc gctggtgatg
     4981 attgttttcg gcgcaacgtt aatgaaagac gcaccaaaac aggaagtgaa aaccagcaat
     5041 ggtgtggtgg agaaagatta cacgctggca gagtcgatgc gtaaaccgca gtactggatg
     5101 ttagcggtaa tgttcctgac tgcctgcatg agcggcctgt acgtgattgg ggtagcgaaa
     5161 gatatcgccc aaagtctggc acaccttgat gtggtttccg cagccaatgc agtaacggtt
     5221 atttccatcg ctaacctttc aggtcgtctg gtgctgggca ttctgtctga caaaattgcc
     5281 cgtatccgtg ttatcaccat tggtcaggtg atttcgctgg tgggtatggc ggccctgctg
     5341 tttgcaccat taaatgcagt gacgttcttt gcagcgattg cctgcgtggc atttaacttt
     5401 ggcggcacta ttaccgtctt tccatcactg gtcagtgagt tcttcggcct caataacctg
     5461 gcgaaaaact acggtgtgat ttacctcggt ttcggtatcg gtagcatttt tggttcgatt
     5521 atcgcctcac tgtttggcgg tttctatgtg accttctacg tgattttcgc cctgctgatt
     5581 ctgtcattgg cgctttctac gacgattcgt cagccagagc agaaaatgtt gcgtgaggcg
     5641 catggctccc tttaatgctc agccgcgagc tactgggctt cagcttccgt gtgcatcaaa
     5701 cgcatcaacg aaatgtataa acaatgactc gtaaaatttg ggccagttaa ctggcccttt
     5761 ttactgcttt ttgtaacgct aaagtctctt ttcaaacttg catttttgta aatttgtgct
     5821 tcatgcacac tctttcccca cactttttcg ctttgctgtg gtctacttat tcgcgcgtgt
     5881 agattttact tatctgacta cctccgcact tttcccctgc cgggcctgaa aagccgttaa
     5941 gcagggtgtt atcacctgtt tgtccagggt ttgtttgcat gagatacatc aaatcgatta
     6001 cacagcagaa gctgagcttt ttgcttgcaa tctatattgg cctttttatg aatggcgcgg
     6061 ttttttatcg ccgcttcggc agctatgcgc acgattttac cgtctggaaa ggcgtttctg
     6121 ctgttgttga actggccgcc accgtactgg tgaccttctt tttactacgt cttctatcgc
     6181 tgtttggccg ccgcagctgg cgtattctgg cgtcgctggt ggtgctcttt tccgcaggtg
     6241 ccagctatta catgaccttc cttaatgtgg tcattggtta tggcatcatc gcttccgtca
     6301 tgaccaccga tatcgacctg tcaaaagaag ttgttggtct gaactttatt ctctggttaa
     6361 tcgccgttag tgcattgcct cttatcctta tctggaataa ccgctgtcgc tacaccttgc
     6421 tccgacaact gcgaacccca gggcagcgta ttcgcagcct ggcggtcgtc gtactggcgg
     6481 gtattatggt ttgggcaccg attcgtttgc tggatatcca gcagaagaaa gtggagaggg
     6541 cgaccggcgt tgatttgccg agttatggcg gtgtcgtagc gaactcttat ctgccatcaa
     6601 actggctttc tgcgttgggg ctgtatgcct gggcgcgggt cgatgaatct tccgataata
     6661 attcattgct taatccggcg aagaagttta cataccaggc tccacaaaac gttgatgaga
     6721 cttatgtcgt gtttatcatc ggtgaaacca cgcgttggga ccatatgggt attttcggct
     6781 atgagcgtaa taccacgccg aaactggccc aggagaaaaa tctggcggcg ttccgtggtt
     6841 actcctgtga taccgcaacc aaactctcac tgcgttgcat gtttgtacgt caggggggcg
     6901 cggaagataa tccgcagcgc acattaaaag aacagaacat tttcgcggtt ctgaagcagt
     6961 taggattcag ttctgacctc tacgctatgc agagcgaaat gtggttctac agcaacacga
     7021 tggcggacaa cattgcttat cgtgagcaga ttggtgcgga gccacgtaat cgtggcaagc
     7081 cggtagatga tatgttgctg gtagacgaaa tgcagcaatc gctagggcgc aacccggatg
     7141 gtaagcatct gatcattctg cataccaaag gttcgcattt taactacacc cagcgttatc
     7201 cgcgtagctt cgcgcagtgg aagccggaat gtattggtgt tgatagcggc tgtaccaaag
     7261 cgcagatgat caactcctat gacaactcgg tgacctatgt ggatcacttt atctccagcg
     7321 tgattgatca ggttcgcgat aagaaagcga ttgtgttcta cgcagctgac cacggtgagt
     7381 caattaatga acgcgagcac ctgcacggca cgccgcgtga actggcaccg ccggagcagt
     7441 tccgcgtacc gatgatggtc tggatgtcag ataaatatct ggaaaatccg gccaatgcgc
     7501 aggcgtttgc gcagctgaaa aaagaagccg acatgaaagt gccacgccgt cacgtagagc
     7561 tgtacgacac catcatgggt tgtcttggct atacttcacc ggatggtgga attaacgaaa
     7621 acaacaactg gtgtcacatc ccgcagacaa aagaggcagc ggctaactaa gaggcctgct
     7681 gactttctcg ccgatcaaaa ggcattttgc tattaaggga ttgacgaggg cgtatctgcg
     7741 cagtaagatg cgccccgcat tcggtgattg gcgcagcttg gtagcgcact tcgttcggga
     7801 cgaaggggtc ggaggttcga atcctctatc accgaccaaa ttcgaaaagc ctgctcagcg
     7861 agcaggcttt tttgcatttg aagcccatga ggatgagaac ctccgggggc
//
LOCUS       DAJVQG010000168         7776 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJVQG010000168 DAJVQG010000000
VERSION     DAJVQG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7776
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.168"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..1780)
                     /locus_tag="OQM83_003486"
     CDS             complement(<1..1780)
                     /locus_tag="OQM83_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCS1521660.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPEWLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQN"
     gene            complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="OQM83_003487"
     CDS             complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="OQM83_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCS1521661.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDINEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="OQM83_003488"
     CDS             complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="OQM83_003488"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCS1521662.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="OQM83_003489"
     CDS             complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="OQM83_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCS1521663.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="OQM83_003490"
     CDS             complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="OQM83_003490"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCS1521664.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5968..7005)
                     /locus_tag="OQM83_003491"
     CDS             complement(5968..7005)
                     /locus_tag="OQM83_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1521665.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYILNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7338..>7776
                     /gene="yhhY"
                     /locus_tag="OQM83_003492"
     CDS             7338..>7776
                     /gene="yhhY"
                     /locus_tag="OQM83_003492"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1521666.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYA"
BASE COUNT         1977 a         2101 c         1871 g         1827 t
ORIGIN      
        1 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
       61 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
      121 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
      181 tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc tcctgtgcta
      241 cctgctttcc ccacccggaa tctccccaac gagaaaggac tcgccccgta gatccttgta
      301 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat gctacctgtt
      361 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagc
      421 cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaac
      481 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
      541 tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa tggttaattc
      601 gttcatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
      661 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
      721 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
      781 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
      841 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
      901 gccctacctg ttggtagtaa ccgacaatag accccggcat ttgataatga atgacgaaac
      961 ctaaatcagg tttatcaaac cccataccta atgccgtcgt cgcgaccagc actttgattt
     1021 tatttgccag cagcgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
     1081 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt aaccactcgg
     1141 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
     1201 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
     1261 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
     1321 ggatatcctc aacgacacga ttattcgctg tcgcggttgt acccagaaca ggggtattcg
     1381 cagtttcaag aaaatcctga ttcgccaacc attcaggtga aatcagcaga caatctacac
     1441 caccccgcag caacttatcg ctaatgcgct gccattcttc cctgtttgta gaattcaacg
     1501 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
     1561 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
     1621 agtacacggc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgctgat
     1681 taaccaccgc atctatagct tcccattgcc cttccctgaa agtagcgctg gtgttactta
     1741 acatccgctg taacagcaat tcagctccgt gtttctccat catcttctcc ctcgtccgtt
     1801 aacgcagtga ttagaacgga taatgttccg gtctttttgc gagagagatc aaaaagatga
     1861 agacaggaga taagagaaat gaattactgc gagcaagctc gcagtaattt gtcgcagtaa
     1921 ttaactcaac agctggaacg caatcatccc aacaatggca ccaacagtgc cgaggatggt
     1981 ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa
     2041 caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg cgatacaaat
     2101 cgacagcgcc gccatttgcg caccggaatt gttcagttgt tcaatgaccg gcatcaccag
     2161 ccccaccgcc gttaaacagg caacggtggc agacccctga atgatgcgca ctgcagctgc
     2221 cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc
     2281 cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag
     2341 cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg
     2401 catgccctga cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt
     2461 aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg
     2521 cgcggcaatg gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa
     2581 tccgaaagat ggcattttac cttcgccgag atgcggctcg ttgatgtcgt caggaatatg
     2641 cagctcaacg taacggctga tgaagttacc ccacagcggc ccggcaataa tcattcccgg
     2701 aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc
     2761 cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa
     2821 taatgggatt atcagcttca ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac
     2881 gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga ccagccccgc
     2941 aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat
     3001 ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac
     3061 caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc
     3121 gagcggcata ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc
     3181 gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa
     3241 aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc
     3301 cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc
     3361 cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt
     3421 gcgcgctttc aggcggcttt caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag
     3481 attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat
     3541 cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca
     3601 gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc attttttcga tattgcgccg
     3661 tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc
     3721 gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt
     3781 agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt
     3841 atcggtaaca ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt
     3901 ataagtgtga gctacttcaa atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa
     3961 acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc
     4021 agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat
     4081 cacctggcca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa
     4141 tcccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcgttgg tacagaacac
     4201 gccatccagc tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta
     4261 agaagaagat tgctcaacca tcacgctata cggcaccaga cctgcatcca gcatcgcctg
     4321 ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat
     4381 gtggcaatgc ccgcgagcaa taatggcggt ggtcatctgg cgtgccgctt caaagttatc
     4441 aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc atgagttcca ccaccgggat
     4501 ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag
     4561 tccgtcgata ttccaggaga gcatggattc gagacgttct tgctccattt ccggtttata
     4621 accgtagtga gccagcatgg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg
     4681 taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct
     4741 ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc
     4801 ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct
     4861 gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt
     4921 tttcatcgtc ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag
     4981 aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc
     5041 atcgcattgt gctaacgacg cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag
     5101 cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg
     5161 gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat
     5221 cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt
     5281 tcaccacctg gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt
     5341 tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa ccatctcgcg
     5401 catccggcga gagcaccagc tgtttgccct gtacggcatc gaagcgacgc tgttcataac
     5461 gcggcgtaat accgttttct tcaggcatga tccagatctg atacagatgc agacgctcgg
     5521 tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact
     5581 cacccgccgg aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca
     5641 gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt
     5701 caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat
     5761 agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt
     5821 catttgcttt gcgtaagtag atcatttgta ttcgccccat gaatgatttc gatgggctta
     5881 gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa
     5941 attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg agggagaggc
     6001 ttgctcaaat ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta
     6061 atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc
     6121 catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac
     6181 accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct
     6241 ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa
     6301 tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata
     6361 aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc
     6421 ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc
     6481 aaggccatag aacgcgccat cctgcggcaa cccaggtttg gtttctgcca ccgggcggta
     6541 atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt caatcgcttt
     6601 tttcgccgtc aggaagcagg aatcaaagcg acgattctga tatggcgtga cggtcagccc
     6661 tttgcttttc gccagtgcaa acagctcttt cgcctgcgca agtgtcggag tgaacggttt
     6721 ttcgaccagc acatttttcc cggcttccag cgcgcgcttc gcgtactcga agtggctgtc
     6781 cgcatgggtg cagacaacaa ccaacttaac atcgggatcg tttagcactt cgtcgagatc
     6841 gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg catggcgacg
     6901 aaaaatatgc gcgacatgcc agctatcctt gcggttaagt atatacggca gatggtaacg
     6961 ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt
     7021 aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac
     7081 gaacacaagc actcccgtgg ataaattgag aacgaaagat caaaaaaaag ccagcacccg
     7141 gctggctaag taatactgga agcaatgtga gcaatgtcgt gctttcaggt tctccgcgag
     7201 ggtcttcctg atcgcgagac aataataatc attctcattc gcacttgtcc aacacttttt
     7261 gcaaaaaaaa tgcatttgac tcgcatttga aagtcaatga tgttgaaagg gacatttacc
     7321 ccaaagagga caaaggaatg agtgagatag taatacgcca cgcagaaaca cgggattacg
     7381 aggccatcag gcagattcac gcccagccgg aggtgtattg caacacacta caggtgcctc
     7441 atccttccga tcatatgtgg caggagcgac tcgccgatcg tcccggcatc aagcaactcg
     7501 tcgcctgtat tgatggagac gtcgtgggcc atctcaccat tgacgtgcaa cagcacccac
     7561 gccgcagtca tgttgccgat tttggtatct gtgtcgactc tcgttggaag aaccgcggcg
     7621 tcgccagcgc cctgatgcga gagatgattg aaatgtgcga caactggttg cgggtagatc
     7681 gcattgaact aaccgtgttt gtcgataacg ccccggcaat taaggtctat aaaaaatacg
     7741 gctttgaaat tgaagggact ggtaagaagt acgcat
//
LOCUS       DAJVQG010000169         7717 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJVQG010000169 DAJVQG010000000
VERSION     DAJVQG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7717
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.169"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..643
                     /locus_tag="OQM83_003493"
     CDS             <1..643
                     /locus_tag="OQM83_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1521667.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            894..2456
                     /gene="hpaB"
                     /locus_tag="OQM83_003494"
     CDS             894..2456
                     /gene="hpaB"
                     /locus_tag="OQM83_003494"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HCS1521668.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHATLQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2474..2986
                     /locus_tag="OQM83_003495"
     CDS             2474..2986
                     /locus_tag="OQM83_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCS1521669.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3379..5529
                     /gene="btsT"
                     /locus_tag="OQM83_003496"
     CDS             3379..5529
                     /gene="btsT"
                     /locus_tag="OQM83_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCS1521670.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5579..5782
                     /locus_tag="OQM83_003497"
     CDS             5579..5782
                     /locus_tag="OQM83_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCS1521671.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5793..6749
                     /gene="yjiA"
                     /locus_tag="OQM83_003498"
     CDS             5793..6749
                     /gene="yjiA"
                     /locus_tag="OQM83_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCS1521672.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6827..>7717)
                     /locus_tag="OQM83_003499"
     CDS             complement(6827..>7717)
                     /locus_tag="OQM83_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCS1521673.1"
                     /translation="DRVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQA
                     ATSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAV
                     PMPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQE
                     VVLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNR
                     GTENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLW
                     LTKQDVEAHKESQEVLHVQR"
BASE COUNT         1785 a         2102 c         2020 g         1810 t
ORIGIN      
        1 tcatgcgttt attacggagt ctgacgccga cggtcatgtg ttgacggtac gggaagatct
       61 gatctggccc ctgctggaag ttctttatcc aggcactcgg gaaaccttcg gcctgccggg
      121 gatttgcctg tcactggcag ataaacccga cgaactggcg gcgctggaac actattggca
      181 actgatagag cgggaatcgg tggaacaact gcctggacgg gaacacaccc tgacgctact
      241 ggcacaggca gtgttcaccc tactgctgcg taatgcaaaa ctcgacgacc atgccgccag
      301 cgggatgcgc ggagaattaa aactgttcca gcgttttaat atgctgattg aaagccattt
      361 tcatcagcac tggacagtac cggattacgc taacgaactg catatcaccg aatcacgcct
      421 cacggacatc tgccgccgct ttgccaaccg tccgccaaaa cggttgattt ttgacaggca
      481 gctgcgagaa gccaagcggc tgctgctgtt ttctgataac gccgtgaaca atattgcctg
      541 gcaactcggt tttaaggatc cagcttattt tgcgcgcttt tttaatcgct tagtcggttg
      601 ctcgcccagt gcttatcgtg ccaaaaaagt acctgtgacg tgaaaatccc ttagccttaa
      661 cgggaaacca ggcaccacct gctattcccc tttctccgcc tggagcaagg ggatttagta
      721 acgtcaaaaa tcgctaaaag cgaccgcgat cacaaaaccg tacccgacct gaaaagtacc
      781 agccattgtc ccaaaagtgt cttcccgtaa tcgcccctgc aagcttcaat tgaataacaa
      841 aaaaaacaca caaaattaac aatcgtattc cgattaatac tgtagaggtc gacatgaaac
      901 cagaagattt ccgcgccagt acccaacgtc cgttcaccgg ggaagagtat ctgaaaagcc
      961 tgcaggatgg tcgcgagatc tatatctatg gcgagcgagt gaaagacgtc actactcatc
     1021 cggcatttcg taatgcggca gcgtctgttg cccaactgta cgacgcgctg cacaaaccgg
     1081 agatgcagga ctctctgtgc tggaacaccg acaccggcag cggcggctat acccataaat
     1141 tcttccgcgt ggcgaaaagt gccgacgacc tgcgccagca acgtgacgcc atcgctgagt
     1201 ggtcacgcct gagctatggc tggatgggcc gtaccccaga ctacaaagct gctttcggtt
     1261 gcgcactggg cgcgaatccg ggcttttacg gtcagttcga gcagaacgcc cgtaactggt
     1321 acacccgtat tcaggaaact ggcctctact ttaaccacgc gattgttaac ccaccgatcg
     1381 atcgtcattt gccgaccgat aaagtgaaag acgtttacat caagctggaa aaagagactg
     1441 acgccgggat tatcgttagc ggtgcgaaag tggttgccac caactcggcg ctgactcact
     1501 acaacatgat tggcttcggc tcggcacaag taatgggcga aaacccggac ttcgcactga
     1561 tgttcgttgc gccaatggat gccaatggcg tcaaattaat ctcccgcgcc tcttatgaga
     1621 tggtcgcggg tgctaccggc tcgccgtacg actacccgct ctccagccgc ttcgatgaga
     1681 acgatgcgat tctggtgatg gataacgtgc tgatcccatg ggaaaacgta ctgatctacc
     1741 gcgatttcga tcgctgccgt cgctggacaa tggaaggcgg ttttgcccgt atgtatccgc
     1801 tgcaagcctg tgtgcgcctg gcagtgaaac tcgacttcat tacagcactg ctgaaaaaat
     1861 cactcgaatg taccggcacc ctggagttcc gtggtgtaca ggccgatctc ggtgaagtgg
     1921 tggcgtggcg caacaccttc tgggcattga gtgactcgat gtgttctgaa gcgacgccgt
     1981 gggtcaacgg ggcttattta ccggatcatg ccacactgca aacctatcgc gtactggcac
     2041 caatggccta cgcgaagatc aaaaacatta tcgaacgcaa cgttaccagt ggcctgatct
     2101 atctcccttc cagtgcccgt gacctgaaca atccgcagat cgaccagtat ctggcgaagt
     2161 atgtgcgcgg ttcgaatggt atggatcacg tccagcgcat caagatcctc aaactgatgt
     2221 gggacgccat tggcagcgag tttggtggtc gccacgaact gtatgaaatc aactactccg
     2281 gtagccagga tgagattcgc ctacagtgtc tgcgccaggc acaaagctcc ggcaatatgg
     2341 acaagatgat ggcgatggtt gatcgctgcc tgtcggaata cgaccagaac ggctggactg
     2401 tgccgcactt gcacaacaac gacgatatca acatgctgga taagctgctg aaataacgca
     2461 gcaggaggtt aagatgcaat tagatgaaca gcgcctgcgc tttcgtgacg cgatggccag
     2521 cctgtcggca gcggtaaata ttatcaccac cgagggcgac gccggacaat gcgggattac
     2581 ggcaacggcc gtctgctcgg tcacggatac accaccatcg ctgatggtgt gcattaacgc
     2641 caacagtgcg atgaacccgg tttttcaggg caacggtaag ttgtgcgtca acgtcctcaa
     2701 ccatgagcag gaactgatgg cacgccactt cgcgggcatg acaggcatgg cgatggaaga
     2761 gcgttttagc ctctcatgct ggcaaaaagg tccgctggcg cagccggtgc tgaaaggttc
     2821 gctggccagt cttgaaggtg agatccgcga tgtgcaggca attggcacac atctggtgta
     2881 tctggtggag attaaaaaca tcatcctcag tgcagaaggt cacggactta tctactttaa
     2941 acgccgtttc catccggtga tgctggaaat ggaagctgcg atttaagctc cagccccttc
     3001 acgcattcgt cgaaggggca tttttattgt gatctcaatc acatatccac ccgatgaata
     3061 attcttgccc aaccgctcac cgccattcct gaccgcttac gtccctaaaa taaccactca
     3121 gttatttacc ttactttacg cgcgcgtaac tctggcaaca tcactgcagg atagcggtca
     3181 atttacctcc tcaaacgcaa cgcaaaccta gaacggcttc ggcctatgat taacaaatta
     3241 ctctatgaat tttggtttgc aggaaggcag caagtgagtg aatccccggg agcttacaac
     3301 agtaagtgac aggggtgaac gaacgcaact gccgcacctg taagccaaaa gacgacgagt
     3361 aaaatgccag gttttactat ggatacgaaa aaactattca agcacatacc ctgggtgatt
     3421 ctcggaatca tcggtgcatt ctgcctcgcg gtagttgcat tacgtcgggg ggagcacgtc
     3481 agcgccctgt ggatcgtggt cgcctctgtg tcggtgtatc tggtggcgta tcgctactac
     3541 agtctgtaca tcgcccagaa ggtgatgaaa ctcgacccca cgcgcgcgac gcctgcggtt
     3601 attaacaacg acggtctgaa ctacgttccg accaaccgtt acgtgttgtt tggtcaccac
     3661 ttcgccgcta tcgccggtgc tggtccgctg gtcggtccgg ttctcgccgc acagatgggc
     3721 cacctgcctg gcacgctgtg gctgctggcg ggggtagtac tggccggtgc ggttcaggac
     3781 tttatggtgc tgtttatctc ctctcgccgt aacggcgcat ctctcggtga gatgataaaa
     3841 gaagagatgg gaccagtacc ggggactatc gcgctgtttg gctgtttctt aatcatgatc
     3901 atcatccttg ccgtcctggc gctgattgtg gttaaagccc tggccgaaag tccgtggggt
     3961 gtcttcaccg tttgctcaac cgtaccgatt gcgctgttta tgggtatcta catgcgcttt
     4021 atccgtccgg gacgtgtggg tgaagtctct gtcattggta tcgtgctgct ggttgcctct
     4081 atctacttcg gtggcgtgat tgctcacgat ccgtactggg gtccggcact gacctttaaa
     4141 gacaccacca ttaccttcgc gctgattggc tatgcgtttg tttccgcatt gctgccggtg
     4201 tggctgatcc tcgcaccgcg cgactatctg gcaaccttcc tgaaaatcgg cgttatcgtc
     4261 ggcctggcgc tgggtatcgt ggtgctgaac ccggaactga aaatgcctgc gatgacccag
     4321 tacattgacg gtactggccc gctgtggaaa ggtgctctgt tcccgttctt gttcattacc
     4381 atcgcctgtg gtgcggtatc tggcttccac gcactgatct cttccggtac gacgccgaaa
     4441 ctgctggcta atgaaaccga cgcgcgtttc attggctacg gcgcaatgct gatggagtcc
     4501 ttcgtggcga ttatggcgct ggttgctgcg tccatcatcg aaccgggtct ttacttcgcg
     4561 atgaacaccc cgcctgctgg ccttggcatc accatgccta acctgcatga aatgggtggc
     4621 gagaacgcgc cgatcatcat ggcgcagctg aaagacgtta ccgcacacgc ggcagcgacc
     4681 gtcagctctt ggggcttcgt gatttcgcca gagcagatcc tgcaaaccgc gaaagacatt
     4741 ggtgagcctt ctgtcctgaa ccgtgcaggt ggtgcgccaa cgctggcggt aggtatcgct
     4801 cacgtgttcc acaaagtgct gccgatggct gacatgggct tctggtatca cttcggtatt
     4861 ctgttcgaag ccctgttcat cctgaccgcg ctggatgcgg gtacccgttc tggccgtttt
     4921 atgctgcaag acctgctggg taacttcatc ccgttcctga aaaaaaccga ctctctggtt
     4981 gccggtatca tcggtactgc gggctgtgtg ggtctgtggg gctacctgct gtatcagggc
     5041 gtggtcgatc cgctgggcgg cgttaagagc ctgtggccgc tgttcggtat ctccaaccag
     5101 atgctggcag ccgtagcgct ggtactgggc accgttgtgc tgattaagat gaagcgcacc
     5161 caatacatct gggtaactgt tgttccggct gtatggctgc ttatctgcac cacctgggcg
     5221 ctgggtctga aactgttcag caccaacccg cagatggaag gcttcttcta catggcaagc
     5281 cagtacaaag agaagattgc taacggtact gacctgacgg cgcagcagat tgccaacatg
     5341 aaccacatcg ttgtgaacaa ctacaccaac gcaggcctga gtattctgtt cctgattgtg
     5401 gtgtacagca tcatcttcta cggtttcaaa acctggcttg cggtgcgtaa cagcgacaaa
     5461 cgtactgaca aagaaactcc gtacgttcca atcccggaag gcggcgtgaa gatctcttcg
     5521 caccactaac cgtgtttagc cccgcttcgg cggggctttg ttcttatcag agtgaactat
     5581 gtttggtaac ttaggacagg caaaaaaata tctcggccag gcggcgaaga tgttgattgg
     5641 cattccagac tacgacaact acgtcgagca tatgaagacc aaccatcccg acaagccgta
     5701 catgagctat gaagaattct tccgcgaacg ccagaacgcg cgctacggcg gcgatggtaa
     5761 aggcggtatg cgctgttgtt aatggagaga gcatgaaccc gattgcagtt accctactca
     5821 ccggtttttt aggcgcgggc aaaaccaccc tgctgcgcca tattcttaac gaacaacacg
     5881 gctacgagat tgccgtgatt gaaaacgaat tcggcgaagt ctccgttgat gatcaactga
     5941 ttggcgaccg cgccacgcag atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct
     6001 ccaacgagct ggaagacgca ctactcgacc tgctggataa tctcgacaag ggcaatattc
     6061 agttcgaccg tctggtcatt gaatgcaccg gcatggccga tcccggcccg attattcaga
     6121 ccttcttctc ccatgagatt ttatgccagc gctacctgct ggacggggtg attgcgctgg
     6181 tggatgcggt acatgccgat gagcagatga accagttcac catcgcccag tcccaggttg
     6241 gctacgccga ccgtattctg ctgacgaaaa ccgacgtcgc aggcgaagca gagaaactgc
     6301 gtgaacgcct ggcgcgcatc aacgcccgcg caccggtcta caccgttacc cacggcgata
     6361 tcgacctggg ccttctgttc aacaccaacg gttttatgct ggaagaaaac gtcgtcagca
     6421 ccaaaccgcg tttccacttt atcgcggaca aacaaaacga tatttcgtcg attgtggtgg
     6481 aactggatta cccggtagat atcagcgaag tttcccgggt gatggaaaac ctgctgctgg
     6541 aatcggcgga taaactgctg cgttacaaag ggatgctgtg gattgacggc gaacctaacc
     6601 gcctgctgtt ccagggcgtc cagcgcctct acagcgccga ctgggaccgc ccgtggggcg
     6661 atgaaaaacc gcatagcacg atggtgttta tcgggattca gttgccggag gatgagattc
     6721 gggcggcgtt tgcgggactc aaaaagtaac cagccctcca acatcacaat acctgagact
     6781 ttatcaccct catattcctc cagaatatga gggcattcgg gcttaattat ctctgaacgt
     6841 gcaacacttc ctgcgactcc ttatgcgcct ccacatcctg cttcgttaac cagagcgact
     6901 tacgaccaaa attttcgtac attttcagct gatcttcata gtgctgatca actgttccat
     6961 cgggagcaat aaacccactc tgaccgggtg cgaccacatc ccaggcaagc accggacgat
     7021 cgcttgtcgt tggtgagaaa acaatcatat cgttttctgt tccacggttt tgatactccg
     7081 cctgatgacg cgtttcttcc gctgcggcct gcggtacacc aaagaaatta tttgcacgga
     7141 acgttaaggc cattgcaggt gttttccagt tactcacatt attgccatag cgtttggaaa
     7201 gagtctccca ggtatcttcc agcgcagcca acacaacctc ctgctgtggt ttcccagcaa
     7261 acagatcaac cgcctgtggg attggtgatt tgtctccctg caccgcctca tacaaaattt
     7321 ttgctccaac acttatattc agcgaaccag ttgggccgtc ctgggttgtt tcgtagccac
     7381 tggcgctgta ccacttatca aatggcatag gtacggcagc cactacggta cgcttcaaca
     7441 tactggtcag ccaaacgttc aggatggcag agcctggctg ctgccaggtt ttaccatcat
     7501 cattaagcaa attgatgcca tcccaacgtg ttaatgtatc caccaactga cggcgcggat
     7561 cgctctgtgt caaaccagat gtcgctgctt gcagagtagg taaaaaaagc ctcaggttaa
     7621 gatcctgacg actggtttgg cgaataacat cccatgcctg atcagcagtt aagcgtggct
     7681 tttgctcaag cagtcggtcg atctccgtaa cgcgatc
//
LOCUS       DAJVQG010000170         7541 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJVQG010000170 DAJVQG010000000
VERSION     DAJVQG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.170"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            447..1400
                     /locus_tag="OQM83_003500"
     CDS             447..1400
                     /locus_tag="OQM83_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCS1521674.1"
                     /translation="MEKFHMFDDRIYQMTNGVKAADRIIYTVMLNEQKFYHDKGQYYT
                     PSYAHLGQYAGITRTSVIACMKQLEAHGLIKKTNVASNQCVIKVYSLDEVPEVLTELT
                     AKEARKAKLESKVTQSVKKDDPLSQNSLPTESKFFTQSVKIFDTYNNSNNKKELINNN
                     KEDDFEEEYFKKGASLCDSHECDSLGIEEAIKETVKTSDLDDLDSEAAKEVVKKEDKI
                     SDVIASSDHKNTQCPATDDELPDVDLDDEEDPEEELKREQRKAEIKERFRNAKLKNKV
                     SDLDDFDDSDDDFTFTPKKKTHTEPKKEKVKVRRVYGGGMF"
     gene            1400..1696
                     /locus_tag="OQM83_003501"
     CDS             1400..1696
                     /locus_tag="OQM83_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521675.1"
                     /translation="MKTYNGERKYRDYLLRRYSISREGYLMKDTHGEVYRIRPKKEGR
                     DYYFVDDVTDLKIDALKFAVLYHYDLWDSVHQLRLKDGDPGNLRATNIIKGKCR"
     gene            2020..2310
                     /locus_tag="OQM83_003502"
     CDS             2020..2310
                     /locus_tag="OQM83_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521676.1"
                     /translation="MTHTVTRLLPEAFERLAAIKAKYPDMKHTALVGEILKESVNLSP
                     FDNVKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADRLEV"
     gene            2531..2746
                     /locus_tag="OQM83_003503"
     CDS             2531..2746
                     /locus_tag="OQM83_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521677.1"
                     /translation="MKTITVQLQTNKAFRYFENLLELYEGWGSIHGKDDIYLHLSAPN
                     YSLKTPVKQSWLKDYGHQMGLLVSDLS"
     gene            2893..3198
                     /locus_tag="OQM83_003504"
     CDS             2893..3198
                     /locus_tag="OQM83_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521678.1"
                     /translation="MNYPMQIMVDQETMNWLIDLNRSGFNIYFMHCFEASTYIDKHII
                     NEIKLGDYVHKLEVTDPYNKRFIIEECNRLDLSPEQLLKLNVFLTYQNELNPYSEVM"
     gene            3674..4384
                     /locus_tag="OQM83_003505"
     CDS             3674..4384
                     /locus_tag="OQM83_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1521679.1"
                     /translation="MTNDIYINGKVIDSFSDWSPQTEHPAITIQRKEHDKRIELEQSL
                     KMSPKTTIFVSPEPQEMPDICKSEALLELEKKYYQLLKEQRLRLDDAYSKVTLMQSAI
                     EPSEFEIQDELSQKPFVYYQYEDNDGFGTFQENVAAVISSLPAGHRIVKMVKASRGAG
                     QFIYMTNKSDEELAEIARQNILASRNKQLDNVKLYLSRELQAMKELVKEYEDAKKIAM
                     QADIEQLTKISSKYAKAI"
     gene            4409..4615
                     /locus_tag="OQM83_003506"
     CDS             4409..4615
                     /locus_tag="OQM83_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521680.1"
                     /translation="MIDLTERYELIKAISERLKEQPNKPKRIKSFTSSKQKNRIRKIA
                     KWCLDKLPSDCQIVGEDGNYYLVR"
     gene            4714..6894
                     /locus_tag="OQM83_003507"
     CDS             4714..6894
                     /locus_tag="OQM83_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCS1521681.1"
                     /translation="MSKFTVDSFIVELSFSENVIKGLQRVEKAALQSAQRIERNLNRA
                     FKVDTKQLDSNLTSSLGQLERKFNKTFDKIEQRARSTKAFQFATRFNDTVNPPKQSRQ
                     PRISGNRAITAAHSVNMSKLGDINPLMAKMFKAQYYSLSGKAGNIGSEKFNAELAKLN
                     QSLRETLNKLRSQTKTASINNTSDAFNNLASNAIKLSGAFYSVMGALNAYKAIMNAGL
                     KRDSAQRAAKFVLGDKASEAEVFIRNLADKTGLNISEGLSSYAKFAAGAQGSMSQEQT
                     QELFGNATAMSRLMGLSNDELNGILRAFEQMASKGKIQAEELRGQLGDRMAGAFKLFA
                     EALGMTATELDAAMKNGKVLSADTLPKVAKQMGLMIDKAGGWAEVTKSTQTALGKLAN
                     NWDDTMVKIFSGSQDELNGFLSSLSNLLSEMGMSSSIAGDAIGGLIDMLKAGVDDIRV
                     FNNTIEGWILQTKKFYYSLDDTKRKLLDEVGDGFINFVKGLAIALSAKTLFSATTGVM
                     NLTRAITTLGTRANQVAGQVATGKGGGKLKGVAGGVGSALAIGYADDGYETALALASM
                     IPQIRGVTLGLYALKKALDFMNQEVVKNANMHPSGVGVGSDFNPVYSGDPNKPNMINE
                     GWSRIFSSMGELFNNATMNIARFNHPELNNIKQDSALTSADIQGLRDEISALSKRIQE
                     PVKVSLGGQVVVRPDEPSFFTFNQSANDQFTQDMLLSSSYPEEE"
     gene            7051..7541
                     /locus_tag="OQM83_003508"
                     /pseudo
     CDS             7051..7541
                     /locus_tag="OQM83_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2484 a         1331 c         1618 g         2108 t
ORIGIN      
        1 cattgcagat gctctcagaa tgcctctgag cacgtcatac aggattgctc aaaggttgag
       61 ctaaaattct cataaatatg gtcatttaca agataggtga ttcagatatt aagaagtatc
      121 cctgtgaaca ataaatgacc aatcagtaat tattctgtga aataattgtt gcaatttata
      181 atcacatctg ttatagtttt tatatggggt gataaaagaa ctcagaacat taagaggtta
      241 caacgttggg aggcgtcagg gtattgacag attttcacgg cctcttattc aatcaaggct
      301 tttccttttg ctgttaatac tcgcgtaatc gctcaggctt ctgagttcct ttatcagtcc
      361 atcttctaat taattcactc cctctggggt tggtcactat acgtggtcag ccctttcttt
      421 gttttatttt taaaaaggaa aatataatgg aaaaattcca catgtttgat gaccgtattt
      481 atcagatgac taatggcgtt aaagctgccg accgtattat ttacactgtc atgttgaatg
      541 aacaaaagtt ttatcacgat aaggggcaat attatacccc ttcatatgct catcttggtc
      601 agtatgctgg aatcacaaga acatccgtga ttgcatgtat gaaacaactt gaggcacatg
      661 gtttaatcaa gaaaactaac gttgcaagca atcagtgtgt tattaaagtt tattcacttg
      721 atgaagtacc ggaggtttta acagagctta cagctaagga agccagaaag gcaaagctgg
      781 agtctaaagt tactcagtca gtcaagaaag atgacccact gagtcaaaat tctttaccca
      841 ctgagtcaaa attttttact cagtcagtca aaatctttga cacgtataat aacagtaata
      901 acaagaaaga acttattaat aacaataaag aagatgactt tgaagaagaa tattttaaga
      961 agggagcttc gctctgtgat tcacatgaat gtgattctct tggtattgaa gaggctatta
     1021 aagagactgt taaaacatct gacttagatg accttgatag tgaagctgct aaagaggtcg
     1081 ttaaaaagga agataaaatc tctgatgtga ttgctagtag tgaccataaa aacacacagt
     1141 gccccgccac tgatgatgaa ttaccggatg ttgaccttga tgatgaagaa gaccctgaag
     1201 aggaattgaa acgtgaacag cgtaaagcag aaattaaaga aagattcaga aatgcgaagc
     1261 taaagaacaa ggttagtgac ctcgatgact ttgatgatag tgacgatgat tttactttta
     1321 caccaaaaaa gaaaactcac accgagccaa agaaagaaaa agttaaagtc agacgtgttt
     1381 atggtggagg aatgttttaa tgaaaactta taatggcgag agaaaatacc gtgactattt
     1441 actcagacgt tacagcattt ctcgtgaagg ttacttgatg aaagatactc acggggaagt
     1501 ttaccggatt cgaccgaaga aagaagggag ggattattat ttcgtcgatg atgtgacaga
     1561 tttaaagatt gacgctttga agtttgcggt gctgtatcat tacgatttat gggattcagt
     1621 ccaccagcta cgattaaaag acggtgaccc cggtaatctg agggccacca atattatcaa
     1681 gggaaagtgc cggtaaggcc accagctatt tatttactga aatctctatc tcaacacgaa
     1741 gaagtttaag tcgaattgta agctttgtct tttttgtaat gactcgattc ataacgcaaa
     1801 acaccaatag cacaataaat accgcaaagc ccaacgagct aaaacgatga gattcaataa
     1861 ttgctgtgaa aaaatcatgc attaattttc cttttgtgaa aggaaacagc acctgtatca
     1921 gcccctacaa ccatagtatc atgtaaatga ggtactgaca atatgttaaa tagatgttaa
     1981 gattgtagta tgagtaagag atttaaggag atttttacaa tgactcacac agttacgcgc
     2041 cttttgccag aggcatttga acgactggca gcaatcaaag ccaaatatcc cgatatgaag
     2101 cacacagcgc ttgttgggga gattttaaaa gagtcggtaa accttagccc tttcgataac
     2161 gttaagggcg tcgcagaggg ttctaaggcc atcagaatca caacgggcca actggaacta
     2221 ctcaaagcac agtccgcagt agcaggttgc tcaatgattc aggtgttaag cgctaaggtc
     2281 catcaactgg cggatagatt ggaggtttaa tgtgttatag gttccaatat tccacacttg
     2341 gccgccacct aatcagacag aatttagcag atgctgaatt tgaagatgtg accctcaaag
     2401 caaacaaacg attaaaaccc tctcaaatcc accagcttaa acacgaatta aagccactgg
     2461 taatactcga ctataacgac cctgaacagc ttgagcaggg aattaagctt atttctgaat
     2521 ggggtgcttg atgaaaacta tcacagtaca acttcagact aataaagcat ttcgttattt
     2581 tgaaaacttg cttgagttat acgaaggatg gggaagtatc cacggcaaag atgatatcta
     2641 tcttcacttg tcagccccta actattctct gaagactccg gttaagcaaa gctggttaaa
     2701 agactacggt caccagatgg ggctattagt ctcagacttg agctaaaaac agcaaaacat
     2761 atctctcagg ctttaccaga cgcaccagaa acgtttttaa gcccctaatc agggtaactt
     2821 agggaatcgc cttaagtggt ctaaaaaagg ggttaaaacg tcttagaatg cgatacagga
     2881 aggattttaa aaatgaatta cccaatgcaa attatggttg accaagaaac catgaattgg
     2941 ttgattgacc tgaacaggtc gggctttaat atctatttca tgcattgttt tgaagcgtca
     3001 acgtatattg acaagcatat aatcaacgaa attaaacttg gtgactatgt tcacaagctg
     3061 gaagtaacag acccatacaa taagcgcttt atcattgaag agtgcaatcg acttgattta
     3121 agccctgagc aattattgaa gctgaatgta ttcttgacct atcagaatga attaaaccct
     3181 tacagcgagg tgatgtaatg acactcgtta ttctctggat gactttaggg ttatttgttg
     3241 gttgctgggt aggtaatacg gtaaataaga attgatgtta ccaatctgtt aaaaagctgt
     3301 tgacaaataa ccttaagcat gagatgatgg taatagggaa gagcaactct tcctgaatta
     3361 aactattaat cattctacat caatgatagt tattctgaaa ggtgcaatgc ttttcgctac
     3421 atgacctttc aggttagtct gagaggtcat ctcgaaaagt actcttgttc attcgtttga
     3481 atcctttttg aatcctttag atgttttaat ttcttcacct tatgaatggc cctttataag
     3541 aaattatcta gggctattca gcttttaaaa agcatacttg gcaaaccgcc ttttaagtct
     3601 cttctaagtt tagataggct gtcaggtgtg ctttttaaaa actgaaacac ttccgcttag
     3661 aagaggaaaa gcaatgacaa atgatattta cattaatggc aaagtgattg atagcttcag
     3721 tgactggtcg ccacagactg aacatccagc tatcactatt cagcgtaaag agcatgacaa
     3781 gcgtattgag cttgagcaat cgctaaagat gtcaccaaaa acaaccatct ttgtgtctcc
     3841 tgagcctcaa gagatgcctg atatttgcaa gagtgaagca ttacttgagc ttgaaaagaa
     3901 atattaccaa ctgctgaaag aacagcgcct tagattagat gacgcttaca gcaaagttac
     3961 cttgatgcaa tcagctattg agccgtcaga atttgagatt caggatgaat tgtcacagaa
     4021 accatttgtc tattaccaat acgaagataa tgatggtttt ggtacttttc aagaaaacgt
     4081 cgcagccgtg attagtagtc ttcctgctgg acatcgaatt gtgaagatgg ttaaggcatc
     4141 aagaggtgct ggtcagttta tttacatgac taataagagc gatgaagaac tcgctgaaat
     4201 tgctagacag aacatcttgg catcacgtaa caagcaactt gataatgtta agttgtatct
     4261 ctcaagagag cttcaggcta tgaaagagct tgtgaaagaa tatgaagatg ctaagaagat
     4321 tgctatgcag gctgatattg agcagcttac gaagatttct agcaaatatg caaaggctat
     4381 ttaattaata attaaaaggt ggtggtgaat gattgattta acagaacgct atgagcttat
     4441 taaagcaatt agtgaacgat tgaaagaaca acccaacaaa ccaaaacgaa tcaaatcatt
     4501 cacctcctct aagcaaaaga atagaatcag gaaaatagct aagtggtgtc ttgataaatt
     4561 accaagtgat tgtcagattg ttggtgaaga tggtaattat tatctggtta ggtaacaaga
     4621 ttggttgggt gaaaatctac gggagagatt atcgagtgat atcggtagtc tttccctttt
     4681 tcgtatatga acacttaaag aggtgaaaca tgaatgagca aattcacagt tgatagtttt
     4741 atcgttgaac ttagtttcag tgaaaacgtt attaagggct tgcaaagagt tgaaaaggca
     4801 gctttgcagt cagcacaaag aattgaacgc aatttgaata gagcgtttaa ggtcgatacc
     4861 aagcaacttg acagcaatct gacaagttct ttagggcagc ttgaaaggaa gtttaataag
     4921 acctttgaca agattgagca gagagcaaga agcacaaaag catttcagtt tgccaccaga
     4981 tttaatgaca ctgttaatcc tccaaaacaa tcaaggcaac cacgaattag cggaaacaga
     5041 gcaatcacag cagcgcatag cgtgaatatg tctaagcttg gtgatattaa tccgctgatg
     5101 gctaaaatgt ttaaagcaca gtattacagc ttaagcggta aggctggtaa tattggaagt
     5161 gaaaagttta acgctgaact tgcaaaatta aatcaatctc ttcgtgaaac gttgaataaa
     5221 ctcaggtcac agacaaagac cgccagcatt aacaatacaa gcgacgcatt taataatctt
     5281 gcaagcaatg cgattaaatt atctggtgca ttttactctg tgatgggtgc tcttaacgct
     5341 tacaaggcca ttatgaatgc aggtcttaaa cgagattccg cacagagagc cgctaagttt
     5401 gtacttggtg ataaagcatc tgaagctgag gtatttattc gaaatttagc agacaagaca
     5461 ggtcttaata tctctgaagg gctatcaagc tacgctaagt ttgcagcagg tgctcagggt
     5521 tctatgtcac aggaacagac acaggagcta tttggtaatg caacggctat gagtcgctta
     5581 atggggcttt ctaacgatga gctaaacggc attttgagag catttgagca gatggcgagc
     5641 aagggcaaga ttcaggctga ggaattacgt gggcagttag gtgaccgtat ggctggtgca
     5701 tttaagctat tcgctgaagc attgggtatg actgctacag agcttgatgc agccatgaaa
     5761 aacgggaagg tactttccgc tgatactctc cctaaagtcg ctaaacagat gggcttgatg
     5821 attgataaag cgggtggctg ggctgaagtc actaagagca ctcagacagc tttaggtaag
     5881 ttagcaaaca attgggatga tacgatggtg aaaatcttct caggttcaca ggatgagcta
     5941 aacggcttct tatccagcct gagtaacctg cttagtgaaa tggggatgag ttcttctatc
     6001 gctggtgatg ctatcggtgg acttatcgac atgcttaaag ctggtgtaga tgatattcgc
     6061 gtattcaata acaccattga aggctggatt ttacagacta agaagttcta ttacagcctt
     6121 gatgacacta aacgaaaact acttgatgaa gtcggtgatg gttttatcaa cttcgtcaaa
     6181 ggtcttgcta tagcgttatc tgcaaagacg ctattcagtg ctactacagg ggttatgaac
     6241 ctgacaagag caatcacaac gttaggtaca agagctaatc aggtggctgg tcaggtagct
     6301 actggtaaag gtggtggaaa actgaaaggt gtagctggtg gtgtaggtag tgctcttgca
     6361 attggttatg ctgatgacgg ttatgaaaca gcgttagcat tagcttcaat gattccacaa
     6421 atcagaggcg tcactcttgg tttatatgca ttgaagaaag cactggattt tatgaatcag
     6481 gaagtagtca aaaacgctaa tatgcatccg tctggggtag gtgtgggtag tgactttaat
     6541 cccgtgtatt ctggtgaccc taataaaccg aatatgatta acgaaggatg gagtagaatt
     6601 tttagttcaa tgggtgaatt gtttaacaat gccacaatga atattgcaag attcaatcat
     6661 ccagagttaa acaacattaa gcaagattca gcattaacga gtgcagatat tcagggtctg
     6721 agggatgaaa ttagtgcttt gagtaagcgt attcaggagc cagtgaaagt ttcacttggt
     6781 ggtcaagttg tcgttcgacc agatgaacca tctttcttta ctttcaatca atcagctaat
     6841 gaccagttca cgcaggatat gctcttaagt tcaagctatc cagaggaaga atgatttaac
     6901 ggtgttctac gtgaataaag caaagttaat acccacacca aaaattattt ttcacgtagc
     6961 tcacgcttaa cagagaaaat acatatatat caaaggcttg gttaaaaatg attaaaatac
     7021 actaagcctt tgaaatcaaa ggagaattta atgatttaca ctaatcagct tcgttacagt
     7081 ggcaaagatg gcatctattt ccacttaaga gacaatgtag atgccttctt aaccttatca
     7141 gcaactgaaa acatggaatt tgatagccct atgcaggtga ctacacagaa catgcaatta
     7201 tgaaggaagc ttattattaa ctcgtcaggg tcaattagta gaaaacttca ttgacactct
     7261 ggaaaactgg cgtgaccaga agcagattat ttctgttatc tgtaaagatg gaattaaaat
     7321 tgacgattcc attattacca actttaaagc ctctaaagat gttactatct ctaacggtct
     7381 tcgtatccag ctaacttttc aggaaattaa ctttaaagca atcgtaggtc aaactgatat
     7441 ttcagcagcc actggcaaaa cggctaccac aaatgacggt ggtgtgacca gtaagaagaa
     7501 tgcaggtaac gtgaccactg aacgcaataa cgcgcctatg c
//
LOCUS       DAJVQG010000171         7429 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJVQG010000171 DAJVQG010000000
VERSION     DAJVQG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7429
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.171"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            69..1055
                     /gene="dcyD"
                     /locus_tag="OQM83_003509"
     CDS             69..1055
                     /gene="dcyD"
                     /locus_tag="OQM83_003509"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCS1521682.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            1070..1738
                     /gene="tcyL"
                     /locus_tag="OQM83_003510"
     CDS             1070..1738
                     /gene="tcyL"
                     /locus_tag="OQM83_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCS1521683.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            1735..2487
                     /gene="yecC"
                     /locus_tag="OQM83_003511"
     CDS             1735..2487
                     /gene="yecC"
                     /locus_tag="OQM83_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCS1521684.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            2717..3439
                     /gene="sdiA"
                     /locus_tag="OQM83_003512"
     CDS             2717..3439
                     /gene="sdiA"
                     /locus_tag="OQM83_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HCS1521685.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(3506..3730)
                     /locus_tag="OQM83_003513"
     CDS             complement(3506..3730)
                     /locus_tag="OQM83_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCS1521686.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(3717..3893)
                     /locus_tag="OQM83_003514"
     CDS             complement(3717..3893)
                     /locus_tag="OQM83_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521687.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            4189..4845
                     /gene="uvrY"
                     /locus_tag="OQM83_003515"
     CDS             4189..4845
                     /gene="uvrY"
                     /locus_tag="OQM83_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCS1521688.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            4842..6674
                     /gene="uvrC"
                     /locus_tag="OQM83_003516"
     CDS             4842..6674
                     /gene="uvrC"
                     /locus_tag="OQM83_003516"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCS1521689.1"
                     /translation="MSDQFDAKAFLKTVTCQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            6731..7279
                     /gene="pgsA"
                     /locus_tag="OQM83_003517"
     CDS             6731..7279
                     /gene="pgsA"
                     /locus_tag="OQM83_003517"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCS1521690.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
BASE COUNT         1837 a         1740 c         1957 g         1895 t
ORIGIN      
        1 ccttttttat ttacgcgttt tcagcgtgca taataagaaa atcacaacaa catacatcgg
       61 aggctgttat gccactgcat aatttaaccc gttttccacg gttggagttt atcggcgcgc
      121 caacgccgct cgaatatctg ccgcgctttt ctgattatct aggacgggaa attttcatca
      181 aacgggatga cgtcaccccc atggcaatgg gcggcaataa attacgtaag ctagaatttc
      241 tcgcggcaga tgctctgcgc gaaggtgccg atacgctgat tactgccggg gtgatccagt
      301 ctaaccatgt gcggcagact gccgcagttg cggcgaaact cggtctgcac tgtgtggcgc
      361 tgctggaaaa tcctattggc acaaccgcag aaaactattt aaccaacggc aatcgcttgt
      421 tgctggatct gttcaatacc cagattgaaa tgtgcgaagc actgaccgat cccaatgccc
      481 aactggaaga gctggcgacg cgagtcgaag cacaaggctt tcgcccgtat gtcattccgg
      541 ttggcggttc taatgctctt ggcgcgctag gttatgtgga gagtgcgctg gaaatcgcgc
      601 aacagtgtga aggggcggtt aatatttcgt cggtggtagt cgcatcgggc agtgccggaa
      661 ctcacgccgg actggctgtt gggctggaac acctgatgcc tgaaagcgaa ctgattggcg
      721 tgaccgtgtc gcgttccgtt gccgatcagt tgccgaaagt ggttaaccta caacaggcga
      781 ttgcgaaaga actggagctg accgcatcag cggaaattat actctgggat gactattttg
      841 cacctggcta cggcgtgccg aacgacgaag gcatggaagc agtgaaattg ctggcgcgtc
      901 tggaaggcat tctgcttgat cctgtgtata ccggaaaagc gatggcgggg ctgattggct
      961 gtatcagtca gaaacgcttc aaagatgaag ggccgattct gtttattcat accggcggtg
     1021 cgcctgcgct gttcgcctat catccccacg tttagctccg ggaacgctca tgcaagaaag
     1081 tatacaactg gttattgatt ccttgccgtt cctgctgaaa ggggctgggt atacgctgca
     1141 actcagtatc ggcggcatgt tttttggctt actgctcggg tttattctcg cgctgatgcg
     1201 cctgtcgcca atctggcccg tgcgctggct ggcgcgtttt tatatctcta tttttcgtgg
     1261 tacaccgctc atcgcacaac tgtttatgat ctattacggc ctgccgcagt ttggtatcga
     1321 attagatccc attccgtcag caatgattgg tctgtcgctg aacactgccg cctatgccgc
     1381 cgaaacactg cgcgccgcca tttcttctat tgataaaggt cagtgggaag cagctgccag
     1441 tattggtatg acgccatggc agactatgcg tcgcgcgatt ttgccacagg cggcgcgggt
     1501 ggcgttgccg ccgctgtcga acagctttat cagcctggta aaggatacgt cgctggccgc
     1561 gacaatccag gtgccggagc tgttccgtca ggcgcagttg attacctcgc gcacgctgga
     1621 ggtgttcacc atgtacctgg cggcttcgct catctactgg atcatggcga cagtattatc
     1681 gacgttgcag aaccattttg agaatcaact taatcgccag gagagagaac caaaatgagt
     1741 gccattgaag ttaagaacct ggtgaaaaaa tttcacggtc agacggtgct gcacggtatc
     1801 gacctcgagg taaagcctgg cgaagtggtg gcaattatcg gtccgagtgg ttccggcaaa
     1861 accacgttgc tacgcagcat aaatctgctg gaacaaccgg aagcgggaac gatcaccgtt
     1921 ggcgatatca ctattgatac tgcacgttca ttaagtcagc aaaaatcgct gatccgccag
     1981 ttgcgtcagc acgtcgggtt tgtcttccag aactttaatt tgtttccgca tcgtacggtg
     2041 ctggaaaaca ttattgaagg gccggtgatc gtcaaaggcg aaccgaaaga agaggccacg
     2101 gcgcgcgctc gcgagctgct ggcgaaagtt gggctggccg gtaaagaaac cagctatcca
     2161 cgtcgtttgt ctggcggtca acagcagcgt gtagcgattg cgcgtgcgct ggcaatgcgt
     2221 cctgaggtga ttttgtttga cgagccaacg tcagcgctgg atccagagct ggtgggcgaa
     2281 gtcctgaaca caatccgcca gctggcgcag gaaaagcgca cgatggtgat tgtgacgcac
     2341 gaaatgagct ttgcccggga tgttgcggac cgggcgattt ttatggacca ggggcggata
     2401 gtcgagcagg gggcagcaaa agcgttattt gctgatcccc agcagcctcg cacccgccag
     2461 ttcctcgaga agtttctact gcaataataa gaaaaaatca gccccgacga ttcacctgtc
     2521 ggggcgggac gccatttcaa gcgtgataaa actgcttaac aaatcagcat aactcattaa
     2581 taacataaga gaatgcgatg gcttgcaaaa gtaattcatt gcctgaataa tataaattat
     2641 atataaatct tatttatgtg atagtttgaa ttatcattat aaatgatact cactctcagg
     2701 ggcgttgcgg tttactatgc aggatacgga ttttttcagc tggcgtcgca cgatgctgtt
     2761 gcgttttcag aggatggagg ccgcagaaga ggtctaccat gaaatagagc ttcaggctca
     2821 gcagctggag tacgattact attcgttatg tgttcgccac ccggtaccat tcacgcgacc
     2881 taaagtggct ttctacacca attaccctga gtcgtgggtt agttattatc aggcaaaaaa
     2941 ttttctcgca attgatcctg tgctgaaccc tgaaaacttt agtcagggcc atttaatgtg
     3001 gaatgatgac ttattcagcg aagcacagcc attatgggaa gccgcgcacg cacatggttt
     3061 acgccgcggt gtcactcagt atttaatgct gccaaatcgg gcgctgggct ttttgtcctt
     3121 ttcccgttgc agcacgcgcg aaatacccat tcttagtgat gaactgcaat taaaaatgca
     3181 gttactggtg cgcgaaagtc tgatggctct gatgcgttta aatgatgaaa tagtgatgac
     3241 gccagagatg aatttcagca agcgcgaaaa agaaattctg aaatggacgg cggaagggaa
     3301 gacatcagca gagatagcga tgattttgtc aatctctgag aatacggtca atttccatca
     3361 gaaaaacatg cagaaaaaaa tcaatgcacc aaataagacc caggtggcct gttacgcggc
     3421 cgctactggc ttaatttgat cgcttttctt tctgcgtgcc agatgcaaaa atcggctgaa
     3481 aggcacgctc tcagccggtt ttatattact gacggtaggc ttgtttaatt tgcttaaccg
     3541 ttttggaaaa tactgcagcc tgggtttcgt cttcgatcag cgcaagctgt ttttccatct
     3601 ttaacatcac gcggcccgcg tcagcttgtc ccatcgcctg tagcatcagc gtcagcatcg
     3661 ctttcaggca ggagacttca tttgccagtt cttgattatt ctcggcagta gaaaaatcag
     3721 gcgtactcat ttattttcct cgtcatgttg caatgaaaat ttgcggtgaa aaatgttaag
     3781 gcggcggagt ataccataag ctttgctaaa aatagcagtg gttgtttttt gagcgtgacg
     3841 tcaacaatgc tattaaatgc gttaatataa tgaccaataa atatttttat catgaatgtt
     3901 ttttgcccgt attgttcggg ttaattaatg ttacatattc agcgggctga ttttcatttt
     3961 tgctgaataa agtcaatttt tgtcacattt catcgccggg cttactgtga aacgatcccg
     4021 gaagccgttg gtgacgggcg tgaccataac tgtggacaat cgaatttaca aaaacgagag
     4081 aaaaatcgaa tacccaccat ttttaacgtt tcaaagttgc aataaaaacc gctaatatac
     4141 gaatgactaa ctatcagtag cgttatccct atttctggag atattccttt gatcaacgtt
     4201 ctacttgttg atgaccacga actggtgcgc gcagggatac gacgcattct ggaagatata
     4261 aagggtataa aagtcgtcgg tgaggcatcg tgcggtgaag acgccgttaa gtggtgccgg
     4321 acaaatgccg ttgacgtggt gctaatggac atgagtatgc cgggcattgg cggtcttgag
     4381 gcgacgcgta aaatcgcgcg ttccacagct gatgtcaaaa tcatcatgct taccgtccat
     4441 acagaaaacc ctttaccagc gaaagtcatg caggccggtg ctgcgggcta cctcagcaaa
     4501 ggcgcggctc cgcaggaagt cgtgagtgcg attcgttctg tctattcagg gcagcgttac
     4561 attgcttctg atatcgctca acaaatggcg ttaagccaga tcgaaccaga aaaaacagaa
     4621 agcccatttg ccagtttgtc tgaacgtgaa ttgcagatta tgctgatgat caccaagggc
     4681 cagaaggtca atgagatctc agaacagctc aatctcagtc cgaaaacggt gaacagctac
     4741 cgctatcgta tgttcagtaa actaaacatt catggcgatg ttgagctgac tcacctggca
     4801 attcgccatg gtctgtgtaa tgcggagaca ttatcaagtc agtgagtgat cagtttgacg
     4861 caaaagcgtt tttaaaaacc gtaacctgcc agccaggcgt ttatcgcatg tacgatgctg
     4921 gtggtacggt tatctatgtc ggcaaagcga aagacctgaa aaaacggctt tccagctatt
     4981 tccgcagcaa cctcgcttcg cgcaaaaccg aagcgctggt cgcccagatc cagcaaattg
     5041 atgtaacggt tacgcataca gaaaccgaag cgctgttgct ggaacacaac tacatcaaac
     5101 tctatcagcc gcgttacaac gttttgctac gcgatgataa atcttatcct tttatcttcc
     5161 tgagtggcga tacccacccg cgtctggcga tgcatcgtgg tgcgaagcat gccaaaggtg
     5221 aatatttcgg cccgttcccg aatggctatg ccgtacgtga aacactggcg ctactgcaaa
     5281 agattttccc cattcgccag tgcgaaaata gtgtttatcg caatcgctcg cgtccgtgtc
     5341 tgcaatatca gatagggcgc tgtctgggac cgtgcgttga aggactggtg agtgaagaag
     5401 aatacgctca gcaggttgag tatgtgcgcc tgtttttgtc tggcaaagat gatcaggtgc
     5461 ttacgcaact cattagtcgt atggaaactg ccagccagaa tctggagttt gaagaagctg
     5521 cacgtattcg cgaccaaatt caggcggtgc gacgcgtcac cgaaaaacaa ttcgtttcca
     5581 ataccggcga cgacctcgac gttattggtg tggcgttcga tgcgggcatg gcttgtgtcc
     5641 acgtattgtt cattcgtcag ggcaaagtgc tcggcagccg cagctatttc ccgaaagtgc
     5701 ctggcggtac ggaactgagc gaggtggtag aaaccttcgt aggccagttc tatttacaag
     5761 gcagccagat gcgcacctta ccgggtgaga tcctgctcga ttttaatctt agcgataaaa
     5821 cgctgctcgc cgattccctt tcagaactgg cgggacgcaa gattaatgtt caaaccaaac
     5881 ctcgcggcga tagggcgcgt tatctgaaac tcgcgcgcac caatgcggcg acggccttaa
     5941 ccagcaaact ttcgcagcaa tctaccgttc accagcgact gaccgcgctt gccagcgtgt
     6001 tgaaattgcc ggaagtgaag cggatggagt gctttgacat cagccatacc atgggcgaac
     6061 aaaccgtcgc ttcctgtgtg gtgtttgatg ctaacggccc gctgcgtgcg gagtatcggc
     6121 gctataacat tacaggcatc acgccgggcg atgattatgc ggcgatgaat caggtgctgc
     6181 gtcggcgtta tggtaaagcc attgacgaca gtaagatccc ggatgtgatc cttatcgacg
     6241 gcggcaaagg ccagcttgcg caggcgaaaa atgtcttcgc cgaactggat gtctcatggg
     6301 ataaaaatca tccgctgcta cttggcgttg ccaaaggagc agatcgtaag gctgggctgg
     6361 aaacgctgtt ctttgagccg gaaggtgagg gattcagttt gccgccagat tctcccgcgc
     6421 tgcatgttat ccagcatatt cgcgatgaat cacatgatca cgcgattggc gggcaccgta
     6481 aaaaacgggc gaaggtcaaa aataccagtt ccctggaaac cattgaaggc gtcgggccaa
     6541 aacgtcggca aatgttgttg aaatatatgg gcggtttgca aggtttacgt aacgccagcg
     6601 tcgaggaaat tgcaaaagtg ccgggtattt cgcaaggtct ggcagaaaag atcttctggt
     6661 cgttgaaaca ttgatgtctc tgtagcaaca taggggtaat cttactgaca acagatagtt
     6721 acccgtcatt atgcaattta atatccctac gttgcttaca ctgttccgtg tcatccttat
     6781 cccattcttt gtattggtct tttatctgcc tgtcacctgg tcgccgtttg ccgccgcgct
     6841 cattttctgc gtcgcggcgg tgactgactg gttcgatggt tttctggcac gccgctggaa
     6901 ccagagtacc cggtttggtg ctttccttga ccctgtagca gataaagttc tcgtggctat
     6961 cgccatggtg ctggtaaccg agcattacca cagctggtgg gtgaccttac cggcagcaac
     7021 gatgatcgcc cgtgaaatta ttatttctgc gctacgcgaa tggatggcgg agttgggtaa
     7081 acgcagtagc gtggctgtct cctggattgg gaaagtgaaa accactgccc agatggtggc
     7141 gttggcatgg ctgctgtggc gtccgaacat ttgggttgag tacgccggta ttgcactttt
     7201 ctttgtggct gcggtactga ctctgtggtc aatgttgcaa tatttgagcg ctgcgcgtgc
     7261 agatttgctt gatcagtgat cgtttcggcg taattttcag caaacgatca aaagtggtga
     7321 aaaatatcgt tgactcatcg cgccaggtaa gtagaatgca acgcatcgaa cggcggcact
     7381 gattgccaga cgataataaa atcaagtgat taactgattg cttgatgaa
//
LOCUS       DAJVQG010000172         7406 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJVQG010000172 DAJVQG010000000
VERSION     DAJVQG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.172"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..234
                     /locus_tag="OQM83_003518"
     CDS             <1..234
                     /locus_tag="OQM83_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCS1521691.1"
                     /translation="SVINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYK
                     TFVCVETAYASETQKVTKEKPAHLAQSIRVAKR"
     gene            complement(285..1139)
                     /gene="yeaE"
                     /locus_tag="OQM83_003519"
     CDS             complement(285..1139)
                     /gene="yeaE"
                     /locus_tag="OQM83_003519"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HCS1521692.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1229..1975)
                     /gene="mipA"
                     /locus_tag="OQM83_003520"
     CDS             complement(1229..1975)
                     /gene="mipA"
                     /locus_tag="OQM83_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCS1521693.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLTGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2411..4345
                     /gene="yeaG"
                     /locus_tag="OQM83_003521"
     CDS             2411..4345
                     /gene="yeaG"
                     /locus_tag="OQM83_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCS1521694.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            4458..5741
                     /locus_tag="OQM83_003522"
     CDS             4458..5741
                     /locus_tag="OQM83_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HCS1521695.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            5888..7363
                     /locus_tag="OQM83_003523"
     CDS             5888..7363
                     /locus_tag="OQM83_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCS1521696.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQKLQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
BASE COUNT         1977 a         1761 c         1710 g         1958 t
ORIGIN      
        1 agcgtgatta atgatgaagc gctgaatcgt attatcgccg taggccacca gcatcatctg
       61 aacgttgtag gctggaaccc gggaccggcg ctttcaatta gcatgggcga tatgccggat
      121 gatggctaca aaacatttgt ttgtgtagaa acggcttacg cttcagaaac gcaaaaagtc
      181 accaaagaga aacctgcaca tctggcgcaa tccattcgcg ttgcgaaacg ttaatttgcg
      241 ttaatgttgt gtgccgggtg cgatgcatcc ggcacgtatc aacatcacac catatccagc
      301 gcagtttttc cttttggtgc cggatacgct ttatccagca tggttaattc cgctgacgaa
      361 agttcgacct caagcacagc cgcattctgt tgaacatggg caatcgtggc cgcttttgga
      421 atcgccatca caccctgatg gctgattacc cacgccaaca atacttgtgc cgagctgata
      481 ttgtgagcat gtgcaatttc gttgactacc gcgtttttta acagtccatt gcgcaaccgc
      541 ccggcctggg ctaatggact gtaagccatc accggcatct gctgttgctg gcaccagggg
      601 agtagatcgt actcaatccc tcgtgaaccg agatggtaaa gcacctgatt agtggcacac
      661 tgatttcccc ccggcagctg ccagagttcc tgcatatcag cataatcaag gttagaaacg
      721 ccccagcggc ggatttttcc ctgggcgatc aatttttcca tcgctgcaac agtctcttca
      781 aaagcgaaac tgccagacct gtgtaataag tagagatcga gataatcagt attgagacgg
      841 cgtaaactgg cttcgcatgc atttatcgct ttttgcccgc cagcattcca cggatagact
      901 ttagagacga gaaagacgtt atcacgcaga ccggttaatg cttccccaac caccttttcg
      961 gcaccgccat cggcatacat ttcggcggta tcaatgaggg ttaaaccgag ctcaatgccc
     1021 gcgcgtagtg cagcaacttc tgttttgcgc tgactggcat cttcgcccat ataccatgtt
     1081 ccctgcccta tggctggcag tgagacatcg ccactaaatt gaatcatttt ttgttgcatt
     1141 gtttcctccc aggtattgca ccaccgtaat gcaaaacagg gcgtgacgcc ctgtttttat
     1201 gcacaaaatg ccctggaaag atgcattatc agaatttgta ggtgatcccg gtagaaatca
     1261 ggccagtcca ggatttatcc accatcgggc tgtcagtaac ttcatcagac agacgggtat
     1321 agcgcgcggt accgtaaaca ctccagtcgc cgaggaagtt gtagctggcg ctcagctcca
     1381 ggtaagggct ccagctgtcg ttcgggttat agccacgcag accgctgcga gcggactctt
     1441 tgcgcgatac gccataatag tattcgttct ggttttcgct gttccactgc acaccgatac
     1501 ccggggtcac tgtcaggccg ccgttggtgt aacgatacaa ccaggccata tcccagacaa
     1561 tgccgttgct gttatccagg gtatcgccag tcagggtggt acgcaggtaa ccgtactggg
     1621 taaagtgagc ataagacaga ccagccatca tggtgctctt acggtcatcc aggtgacgca
     1681 tttggtgatc gccactgtct ttcgctttga agtaaagcgg cgaccaataa gcggtaattg
     1741 aaagtttgtc cgttgcgtca ttccacaggt agtaaccacc acctaagcca cggaaccaga
     1801 agttatcgcc ttcatagttg attaccggta ctgggtaaac atcggtatcg taatctttat
     1861 atgggtgttc aacgacaccg acgcctgcgc ccagggaaaa tttaccttca gcgtgcgcta
     1921 cgcctgcaga cgttgcgata agcactccaa gtgccagaag tttgagtttg gtcacaatta
     1981 atcattcctt aaacaaatgt ttagcgggcg acaaagttta cccgtcaata catcgaaacc
     2041 caaccttttt acgttttcat tttttaaagt aactgtttaa ttttcctgac gcggatgaca
     2101 ccgcgcttac agccaaatga atttagcgtt actggcgagc cttgtcttta cattaattat
     2161 gcaaaattta tggatgagtt gttgatatgc cattgaaatt aagaaagccg tgcaggcaag
     2221 ttttccattt gccatctacg cttaattttg aaggtgtatc accgggcacg ttgttctcat
     2281 cgtcgataaa atggcatgag agttgctgtg ttttagcaag agacgtcgtt cagtttacct
     2341 cttccgggag cctctactat tcatatgaac ggctcttaac ctgtgctaaa aaacgaaagg
     2401 acggcatacc atgaatatat tcgatcacta tcgccagcga tatgaagctg ccaaggacga
     2461 agagttcaca ctgcaggagt ttcttaccac ttgtcggcaa gatcgcagtg cttatgccaa
     2521 cgcggctgag cggctattga tggctatcgg tgagcctgtc atggtcgata cagcccagga
     2581 acccagactt tctcgactct tttctaaccg ggtcattgca cgttatccgg catttgaaga
     2641 gttttacggc atggaagacg cgattgaaca gattgtctct tatctgaaac acgcggctca
     2701 ggggctggaa gagaagaaac aaatcctgta tctgctgggg cctgtgggtg ggggtaaatc
     2761 atcgcttgct gagcgactga aatcattaat gcagctcgta ccgatttatg tattgagcgc
     2821 caacggtgag cgtagcccgg tcaacgatca tccgttctgt cttttcaatc cgcaggaaga
     2881 tgcgcagatt ctggaaaaag agtatggcat tcctcgccgc tatctcggca ccatcatgtc
     2941 gccgtgggcg gcaaaacgcc tgcatgaatt tggtggcgat atcactaagt tccgggtagt
     3001 gaaggtctgg ccgtcaattc tgcaacaaat tgctatcgcc aaaacagaac ccggtgatga
     3061 gaacaaccag gacatctccg cgctggtagg gaaagtcgat attcgtaaac tcgaacacta
     3121 cgcgcagaat gacccggacg cctacggcta ttccggtgcg ctgtgccgcg ccaatcaggg
     3181 gatcatggaa tttgtcgaga tgtttaaagc accgattaaa gtgctgcatc cgctgttaac
     3241 cgctactcag gaaggtaact acaacgggac ggaaggtatc tccgccctgc cgttcaacgg
     3301 gattattctc gcccactcga acgaatccga atgggtcact ttccgtaata acaaaaacaa
     3361 cgaagccttc ctcgaccgtg tttacatcgt gaaggtgccg tattgcttac gcatttccga
     3421 agagatcaaa atctacgaga aattgcttaa tcacagtgaa ttgactcacg ccccatgcgc
     3481 ccctggcacg ctggaaacac tgtcacgttt ttccattctt tcgcgcctga aagagccaga
     3541 aaactccagc atttattcaa agatgcgggt ttatgatggc gaaagtctga aagacaccga
     3601 tcccaaagcc aagtcgtatc aggaatatcg tgactacgcc ggtgtcgatg aagggatgaa
     3661 aggtctgtcg acgcgttttg cgtttaagat cctctcccgc gtgttcaact tcgatcatgt
     3721 agaagtggcg gcaaacccgg tccatctgtt ctacgtccta gaacagcaga tcgagcgcga
     3781 gcagttcccg caagagcagg cagaacgcta tctggagttc ctgaaaggtt atctgatccc
     3841 gaaatatgcc gagtttatcg gcaaagagat ccagacggcc taccttgaat cctattccga
     3901 atatgggcaa aacattttcg accgttatgt tacctacgcg gatttctgga ttcaggatca
     3961 ggagtatcgc gatccggata ccgggcagct gtttgaccgc gagtctctta acgccgagct
     4021 ggagaaaatc gagaaaccgg cggggatcag taatccaaaa gatttccgca acgagattgt
     4081 taacttcgta ctgcgcgcca gagcgaataa cagcggacgc aatccgaact ggaccagcta
     4141 tgaaaaactg cgcacggtta tcgagaagaa aatgttctcc aataccgagg agctgttgcc
     4201 ggttatctcg tttaacgcca aaacgtcaac cgacgagcag aagaaacacg acgactttgt
     4261 cgaccgtatg atggaaaaag gctacacccg taaacaggtg cgtttactgt gcgaatggta
     4321 tttgcgcgta cgtaaatcgt cttaacaacc ctggcccggt acacatgcta ccgggcctat
     4381 aacgacagcg aaccgtgggc cagagaaatg gcaacacagg cgtagcatac agttggcaaa
     4441 tgtagtacgg ggggcatatg accgggttta ttgaccggcg tctgaacggc aaaaacaaaa
     4501 gcatggtgaa tcgccagcgt tttttacgcc gttataaagc gcaaattaaa cagtcgatct
     4561 ccgaggccat taataagcgt tcggtgactg acgtcgacag cggcgaatcc gtatccattc
     4621 ccacggaaga tattagcgaa ccgatgtttc atcaggggcg tggcggcctg cgccaccgcg
     4681 tacatccggg caatgaccat ttcgtccaga acgaccgaat tgaacgtccc cagggtggcg
     4741 gcggaggttc cggcagtggt cagggccagg ccagccagga tggtgaaggt caggatgaat
     4801 ttgtctttca gatttcgaaa gatgagtatc ttgatctgct ctttgaagat ttggccttac
     4861 cgaatctgaa acaaaaccaa caacgccagc tgaccgaata taaaacgcat cgggcgggtt
     4921 ataccgctaa cggcgttcca gccaatatca gcgttgtgcg ttccttgcag aactcactgg
     4981 cgcgacgcac agccatgacg gcgggcaagc gacgggaact tcatgcgctg gaagagaatt
     5041 tggccatcat cagcaacagt gaacctgcgc aactgctgga agaggaacgt ctgcgcaaag
     5101 aaattgcaga attacgtgcc aaaattgaac gcgtcccttt tattgacacc ttcgatttac
     5161 gttacaagaa ctacgagaag cggcccgatc cctccagcca ggcagtgatg ttttgcctga
     5221 tggacgtttc cggttcaatg gatcaatcca ctaaagatat ggctaagcgt ttttatattc
     5281 tgctgtatct gttcctcagc agaacgtata agaacgtgga agtcgtatac atccgccatc
     5341 atacccaggc gaaagaagtc gatgaacatg agtttttcta ctcgcaggaa acaggcggca
     5401 ccattgtttc cagcgccctg aaactgatgg atgaggtagt gaaagagcgt tataacccgg
     5461 cacagtggaa tatttacgct gcacaagcat cggacggcga taactgggcc gatgactctc
     5521 cgctttgcca tgaaatcctg gcgaaaaaat tattacctgt tgttcgttat tacagctata
     5581 tcgaaattac ccgtcgtgca catcagacat tgtggcgaga atatgagcat ctgcaatcta
     5641 ctttcgacaa ctttgcgatg cagcacatcc gcgaccagga tgatatttat ccggtgttcc
     5701 gtgaactgtt tcataaacaa aatgcaacag ctaaagacta aaactatcag ccaggtcatt
     5761 atcgcctggc tgatttttag cttactgtaa attatctcat ttattacata cattagctta
     5821 caatcgcttt aaatatgaca gcataacctt tacataattt agttccagaa aacaatcatt
     5881 cggaaaaatg attcagtcaa cacgtatttc catggggtta ttctttaaat attttttatc
     5941 gttaacgaaa attgatcctg gtcaaaacta tatatctctg ccatcaataa aatccagcac
     6001 tcacattgct ctccttttta tggtttctat gggtacacaa aaattaaaag ctcaaagctt
     6061 ttttattttc agtttattgc tgacgttaat tttattttgc attactacct tatataacga
     6121 aaacacaaat gtaaaactca tcccacagat gaattacctg atggttgttg tggctttgtt
     6181 tttccttaac gccgtcattt ttcttttcat gttaatgaaa tatttcacta acaaacaaat
     6241 tttaccaaca ctcattttaa gccttgcatt tttaagtggc cttatctatt tagttgaaac
     6301 cattgtaatt atccataaac caattaacgg cagtacactg atccagacaa agtcgaatga
     6361 tgtttctatt ttctatattt tccgccaact cagttttatt tgtttaacct cgctggcact
     6421 cttttgttat ggaaaagaca acatccttga caacaataag aaaaaaacgg gaatcctgtt
     6481 gctggcgctg attccttttt tagtttttcc ccttctggca cacaatctga gcagttataa
     6541 cgctgactat tctttgtatg tcgtcgacta ctgtccggac aaccatactg cgacctgggg
     6601 aatcaactat acaaaaatat tggtttgtct gtgggcattt ttactgttct ttattatcat
     6661 gcgtacacga ttagccagcg aactatggcc gttaatagca ttattatgtc tggcatcgct
     6721 atgctgcaac ttacttctac tgactctgga tgagtataat tacaccatct ggtatatcag
     6781 tcgcgggatt gaagtttcca gtaaactgtt tgttgtgtct tttctgattt ataacatttt
     6841 tcagaaactg caactctcca gcaaactggc agttcatgat gtgctgacca atatttataa
     6901 tcggcgctac tttttcaaca gcgtagagtc attattgtcg agacctgttg ttaaggactt
     6961 ctgtgtcatg ctggttgata ttaatcagtt caaacgcatc aatgcccaat ggggacatcg
     7021 tgtgggtgat aaagtgctgg tttcaattgt cgatattatc cagcaaagca tccgccccga
     7081 tgatatttta gcgcgactgg agggtgaggt gtttggcttg ctatttaccg aactcaatag
     7141 tgcccaggca aaaatcattg cggaacgtat gcgtaaaaat gtcgaactcc tgaccggctt
     7201 tagtaacaga tatgatgttc ctgaacaaat gaccatcagt attggcacgg ttttttcaac
     7261 gggtgacacg cgtaatatct cgcttgtcat gacggaagca gataaagcct tacgcgaagc
     7321 gaaaagcgag gggggcaaca aagtgattat tcatcatatt taagtgcaaa aatattcaga
     7381 gccatgcttt ttgcgtggct tttgca
//
LOCUS       DAJVQG010000173         7380 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJVQG010000173 DAJVQG010000000
VERSION     DAJVQG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7380
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.173"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..239
                     /locus_tag="OQM83_003524"
     CDS             <1..239
                     /locus_tag="OQM83_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HCS1521697.1"
                     /translation="LLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIVQLLLEHG
                     ASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            279..1142
                     /locus_tag="OQM83_003525"
     CDS             279..1142
                     /locus_tag="OQM83_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCS1521698.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            1132..2679
                     /gene="fdrA"
                     /locus_tag="OQM83_003526"
     CDS             1132..2679
                     /gene="fdrA"
                     /locus_tag="OQM83_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HCS1521699.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            2679..4037
                     /locus_tag="OQM83_003527"
     CDS             2679..4037
                     /locus_tag="OQM83_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCS1521700.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAGPPITWEKMCGAMKGAVTGAL
                     VFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGVTSASMFMHIVKNKTYGNIAY
                     TNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDAMKIIGEIDLRLMLAQALHMG
                     DECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEFVASSDYFSGPTWMAMCKAAM
                     DAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPAQQVIGPMFAGYKPEDSGLDI
                     GDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSRQMREITLGENPNVTIPLLGF
                     MGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAGIVHPPFACFEKAILGWCERY
                     GV"
     gene            4180..5130
                     /locus_tag="OQM83_003528"
     CDS             4180..5130
                     /locus_tag="OQM83_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HCS1521701.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADMVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            5140..6522
                     /locus_tag="OQM83_003529"
     CDS             5140..6522
                     /locus_tag="OQM83_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCS1521702.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTYVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLKQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            6899..>7380
                     /gene="yahK"
                     /locus_tag="OQM83_003530"
     CDS             6899..>7380
                     /gene="yahK"
                     /locus_tag="OQM83_003530"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HCS1521703.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     "
BASE COUNT         1767 a         1886 c         2143 g         1584 t
ORIGIN      
        1 agcttttggc gcatacggag attaacgtta accagaccaa ccatgtaggc tggacgccgc
       61 tactggaagc gattgtgctt aatgatggtg gtattaaaca gcaggcgatt gtgcagttat
      121 tgctggaaca cggtgccagc ccgcatctga ccgataaata tggcaaaacg ccactggaac
      181 tggcgcggga acggggcttt gaagagattg cgcagttact gattgccgca ggtgcataaa
      241 ccgggaggct ggctatcaac acaccagaaa aacggtgtgt gtgggcgcta actgcggatg
      301 cggattttct ggcgcagcgg gggcaaggac aggttgaaca ggtctttgcc agagcggtaa
      361 atatcgcact cccggctcgc cagcagttgc tgacgctgct ttgtgaagag tacgacaatg
      421 cgccaaacag ttgtcggttg gcactcactc actttgatga tctgttccgg catggtgata
      481 aggttcagtt tgacgatcaa ggtattacgg ttggtcaaca tcttcatata gagatgagtc
      541 gttgtcggcg ttggctgtcc ccaaccttgc aaatgaccgc tgtgaatttt caccttatcg
      601 cctggctaca gtggcacgac attattcatc agcacctggg ggaaaatgaa accctgttta
      661 attatcgcgg cgataatccg ttttatcagg cgttaaataa agaattacat attaaacgac
      721 gggcagttat tcagacagta aacgataagc aaaatatcgc cgcagcggtc gccagtatga
      781 ttgggttagg gattggcctt acgccatcag ccgatgatta tttaacaggt ctggcgctta
      841 ttttatttat tcccgggcat ccggcagaaa aatacaaaga ggaattttat cgcggtctgc
      901 tacgcggcag aaataacacc acgttattaa gtgccataac gctggaagcc gcattacaac
      961 aacgctgccg ggaaaatatt catcgcttta ttcacaacat tatttatggc gtccctggga
     1021 attcaactca ggcaatagaa aaaattaaac atattggctc cagttccggc tgcgacatgc
     1081 tgtatggcat ggccgatggt tgtgcgctga gccaaaccta cggagggaat tatgtcagtt
     1141 aaaatagtca ttaaaccgaa tacctatttt gattctgtct cgctgatgtc tatctccacg
     1201 cgtgcaaata aactcgacgg cgtcgagcag gcatttgtgg cgatggcgac cgaaatgaac
     1261 aaaggtgtgc tgaaaaattt aggactgctg acgccgacgc tggaacaggc gaaaaacggc
     1321 gacctgatga ttgtcatcaa tggtaaatcg ggtgcggaca acgagcagtt gctggtggag
     1381 attgaagaac tgttcaacac caaagcgcaa agcggctcgc acgaggcgcg ttacgccact
     1441 attgccagcg ccaaaaagca tatcccggaa agtaacctgg cggtgatttc ggtcaacggt
     1501 ctgtttgccg ctcgcgaagc gcgtcaggcg ctgcaaaacg atctcaacgt gatgctgttt
     1561 tccgataacg tctcagttga agatgaactg gcgctcaagc agttggccca cgaaaaaggg
     1621 ctgctgatga tggggccgga ctgcggcacg gcgattatta acggcgcggc gctctgtttt
     1681 ggtaacgccg tgcgtcgcgg caatatcggt attgttggcg catccggcac cggcagccag
     1741 gagctgagcg tccgcattca tgaatttggt ggcggtgttt cacaactgat tggcaccggc
     1801 gggcgcgacc tgagcgagaa aatcggcggc ctgatgatgc tcgacgccat cgggatgctg
     1861 gaaaacgatc cgcaaactga aattatcgca ctgatttcaa aacctcccgc gcctgcggtg
     1921 gcccgcaaag tgctggaacg cgcacgtgcc tgccgcaagc cggtggtggt ctgcttcctc
     1981 ggtcgcgttg aaacgccggt ggatgagcag gggctacagt ttgcccgcgg cagcaaagag
     2041 gcggcgctaa aagcggtgat gctctccggc gtgaaacagg aaaatctcga cctgcatacg
     2101 cttaaccagc cgttgattgc tgatgtgcgt gcgcgtctgc aaccgcagca gaaatacatt
     2161 cgtggcctgt tctgcggcgg cacgctgtgc gacgaaacca tgttcgcggt gatggaaaaa
     2221 catggcgatg tctacagcaa cattcagccc gatccggaat tccgcctgca agatatcaac
     2281 cgcagcatca aacacacctt cctcgacttt ggcgatgacg acttcacgaa cggcaagccg
     2341 catccaatga ttgaccccac caaccgcatc agtcgcttga tcgaagaggc gcgcgatcca
     2401 gaagtggcgg tgatcgtgat ggattttgtg ctcggatttg gatcgcatga agatccggtc
     2461 ggctccacca tcgaggcgat caaagaagcg aaagcgatcg ccgctgccga aggacgcgag
     2521 ttgatcattc tcgcctatgt gctgggtacc gatcttgata cgccatcgtt agaacaacaa
     2581 agccagatgc tgcttgatgc cggagtgatt ctggcgagca gcagcaccaa taccggattg
     2641 ctggcgcgtg aatttatctg caaaggggag gaagcctgat gagccagtca ctgtttagcc
     2701 aaccattgaa cgttattaac gtcggcatcg ccatgtttag cgatgacctg aaaaagcagc
     2761 atgtagaagt gactcaactc gactggacgc cgccggggca gggcaatatg caggtggtgc
     2821 aggcgctgga gcgcattatc cagtcgcatc cggtgctgat tggttttgat caggcgatta
     2881 acgtggtgcc gggcatgacg ccgaaaacca ttcttcacgc cgggccgccg atcacctggg
     2941 aaaaaatgtg cggcgcgatg aaaggcgcgg tcaccggagc gctggtgttc gaaggactgg
     3001 cgaaagatct cgacgaggcg gctgaactgg cggcctccgg ggagatcacc ttctcgccgt
     3061 gccacgaaca cgactgtgtg ggatcgatgg cgggcgttac ctcggcctcg atgtttatgc
     3121 acatcgtgaa aaacaaaacc tacggcaaca tcgcttacac caatatgagc gagcagatgg
     3181 cgaagatttt gcgtatgggt gccaacgatc agagcgtgat cgaccgcctc aactggatgc
     3241 gcgatgtgca ggggccaatg ctgcgcgacg cgatgaaaat tatcggtgag atcgatctgc
     3301 gcttaatgct ggcgcaggcg ctgcatatgg gcgatgagtg ccataaccgc aataacgccg
     3361 ggacgacgct gctgattcag gcactgacgc cggggatcat tcaggcaggc tattccgtcg
     3421 agcaacagcg cgaagtgttc gagtttgtcg ccagcagcga ctacttctcc ggcccgacgt
     3481 ggatggcgat gtgtaaagcg gcgatggatg cggcgcacgg catcgaatac agcaccgtgg
     3541 tcaccaccat ggcgcgtaac ggcgtcgagt tcggcctgcg ggtcagcggc ctgccggggc
     3601 aatggtttac cggcccggcg cagcaggtga tcggcccgat gtttgccggt tataagccgg
     3661 aagattcggg gctggatatc ggcgacagcg ccatcaccga aacctacggt attggtggat
     3721 ttgctatggc gaccgcgcct gcaatcgtcg cgctggtggg cggcacggtg gaagaggcta
     3781 tcgatttctc ccgtcagatg cgtgaaatca ctctcggtga aaaccccaac gtcaccattc
     3841 cgctgctcgg ttttatgggc gtgccgtcgg caatcgacat cacccgcgtg ggtagcagcg
     3901 gcattctgcc ggtgatcaac accgccatcg cccataaaga tgcgggcgtc ggcatgattg
     3961 gcgcgggcat tgtgcatcca ccattcgctt gcttcgagaa agccattctc ggctggtgcg
     4021 aacgttacgg cgtctgactt acatgtttat gccggatgcg gcgtgaacgc cttatccggc
     4081 ctacggatgg cccgagaatt gtaggcctga taagcgtagc gcatcaggca gtctggcgtt
     4141 ggtcataacc tcatcaccct ctgtagcgga cataacaaca tgaaagagct tgtggtcgtt
     4201 gccattggtg gcaacagcat tatcaaagat aacgccagcc agtcgattga gcatcaggcg
     4261 gaggcggtga aagccgtggc cgatatggtg ctggaaatgc tggcttccga ttacgacatt
     4321 gtgctgaccc acggtaacgg gccgcaggtc gggctggatt tacgccgtgc ggagattgcc
     4381 cacgagcgcg aagggctgcc cttaacgccg ctggcgaact gtgtggcaga tacgcagggc
     4441 ggcatcggct atctgatcca acaggcgctg aataaccgac tggcgcgtca cggcgagaaa
     4501 aaagccgtca ccgtggtaac tcaggtggaa gtggataaaa acgatccagg ttttgcccat
     4561 cccaccaagc ccattggtgc attcttcagt gaaagccagc gtgacaaact acaaaaggca
     4621 aaccctgact ggtgttttgt tgaagatgcc ggacggggct atcgccgcgt ggtcgcctcg
     4681 ccggaaccga aacgtattgt cgaagcaccc gccattaagg cgctgatcca acaaggtttt
     4741 gtcgtaattg gcgcgggcgg cggtggaatt ccggtagtgc gtactgaagc gggggattac
     4801 caaagcgtgg acgcggttat tgacaaagat ctctctaccg cgctgctggc ccgtgaaatt
     4861 cacgccgaca ttctggtgat caccaccgac gtcgaaaaag tctgtatcca ctttggtaaa
     4921 ccgcagcagc aggcgctgga tcgggtggat attgccacca tgactcgcta tatgcaggaa
     4981 gggcattttt ctcccggcag catgttgcca aaaatcatcg ccagcctgac gttcctggag
     5041 cagggcggca aagaagtgat tatcaccacg ccggaatgcc tgcctgcggc gctgcgcggt
     5101 gaaacgggca cccatattat taaaacgtaa ggacgtagga tgaaagaaag caatagccgc
     5161 cgtgaatttc tgagccagag cggtaagatg gtcaccgccg ccgcgctgtt tggtacctat
     5221 gtgccgctcg cccatgcggc ggtagctggc accctaaact gcgaagcgaa caacaccatg
     5281 aaaatcactg acccgcatta ctatctcgat aacgtgctgc tggaaaccgg ttttgactac
     5341 gaaaacggtg tggcggtgca aacccgcacg gcgcgccaga ccgtggagat tcaggacggc
     5401 aaaattgtcg ccctgcgcga gaacaagcag catccggacg ccacgctgcc gcactatgac
     5461 gctggcggta agttgatgct acccaccact cgcgacatgc atattcatct cgacaaaacc
     5521 ttctacggcg ggccgtggcg ctcgctcaat cgtccggcag gcaccactat ccaggacatg
     5581 atcaaactca agcagaaaat gctgccggag ttacaaccgt atacgcagga gcgggcggaa
     5641 aaactgatcg atctattgca gtcgaaaggc accaccattg cccgcagcca ctgcaatatc
     5701 gaaccggttt ccggcctaaa aaatctgcaa aatttgcagg cggtgctggc gcggcgtcag
     5761 gcgggcttcg agtgtgaaat cgtcgccttc ccgcagcacg gtttgctgct gtcgaaatcg
     5821 gaacccttaa tgcgcgaagc gatgcaggcg ggtgcgcatt acgtcggcgg gctggacccg
     5881 accagtgtcg atggcgcgat ggaaaaatcc ctcgacacca tgtttcagat tgcgctggac
     5941 tacgacaaag gtgtcgatat tcacctgcac gaaaccactc cgtcgggcgt ggcagccatc
     6001 aattatatgg ttgaaacggt agagaaaacg ccgcaactga aaggtaagct gaccatcagc
     6061 cacgcctttg cgctggctac gctcaacgaa caacaggtcg atgcgctggc gaatcggatg
     6121 gcggcgcagc aaatttctat cgcctcgacg gtgccgattg gcacgctgca tatgccgctc
     6181 aaacagttgc acgacaaagg cgtaaaagtg atgactggca ctgacagcgt tatcgaccac
     6241 tggtcgcctt atggtctggg cgacatgctg gaaaaagcca atctctacgc gcagctctat
     6301 attcgtccta acgaacagaa cctctcccgt tcgctgtttc tagccactgg cgatgtattg
     6361 ccgctgaatg aaaaaggcga gcgtgtatgg ccaaaagcgc aggatgatgc cagctttgtg
     6421 ctggtggacg cctcctgttc cgccgaggcg gtggcgcgta tctcgccgag aaccgcaacg
     6481 ttccataaag ggcaactggt gtgggggagt gtggcaggtt gatgcgcatt tgaaacgcct
     6541 gcaacggtga attgtaagag atttaagccc cagggagtgg ggcgacctca atatgatagt
     6601 gtcggcagaa atggacatta tctttgagga atatgccctt attgacgttg tgtagataac
     6661 tatttgtcta cgtgaaaaga tcatcagttt tgccgatttt agtcttttac ggatagcaaa
     6721 tatcacactt ataggcgctc gccttatggg gaacgttgct gctctgtttc attcaggttc
     6781 gcggcatgtt ggggctggat cgggtggttt aaatattggc tatattcaat ggacgcgttt
     6841 tgccgcgatg acatatcagg cgtcgccaaa tacacatagc taatcaggag taaacacaat
     6901 gaagatcaaa gctgttggtg catattccgc taaacaacca cttgaaccga tggatatcac
     6961 acggcgtgaa ccgggaccga atgatgtcaa aatcgaaatc gcttactgtg gcgtctgcca
     7021 ttccgatctc caccaggtcc gttccgagtg ggcggggacg gtttacccct gcgtaccggg
     7081 tcatgaaatt gtggggcgtg tggtagccgt tggtgatcag gtagaaaaac atgcgccggg
     7141 cgatctggtc ggtgtcggct gcattgtcga cagttgtaaa cattgcgaag agtgtgagga
     7201 cgggctggaa aactactgtg atcacatgat cggcacctat aactcgccga cgccggacga
     7261 accgggccat actctgggcg gctactcaca acagatcgtc gttcatgagc gatatgttct
     7321 gcgtattcgt cacccgcaag agcagctggc ggcggtggct cctttgttgt gtgcagggat
//
LOCUS       DAJVQG010000174         7364 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJVQG010000174 DAJVQG010000000
VERSION     DAJVQG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.174"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..89)
                     /locus_tag="OQM83_003531"
     CDS             complement(<1..89)
                     /locus_tag="OQM83_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HCS1521704.1"
                     /translation="MTLTRREFIKHSGIAAGAQVVTSAAPLPA"
     gene            complement(114..1214)
                     /locus_tag="OQM83_003532"
     CDS             complement(114..1214)
                     /locus_tag="OQM83_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCS1521705.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKSFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(1602..2348)
                     /gene="cutC"
                     /locus_tag="OQM83_003533"
     CDS             complement(1602..2348)
                     /gene="cutC"
                     /locus_tag="OQM83_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCS1521706.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(2362..2928)
                     /locus_tag="OQM83_003534"
     CDS             complement(2362..2928)
                     /locus_tag="OQM83_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1521707.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            3144..4880
                     /gene="argS"
                     /locus_tag="OQM83_003535"
     CDS             3144..4880
                     /gene="argS"
                     /locus_tag="OQM83_003535"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCS1521708.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            5057..5419
                     /locus_tag="OQM83_003536"
                     /pseudo
     CDS             5057..5419
                     /locus_tag="OQM83_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
     gene            complement(5423..6493)
                     /locus_tag="OQM83_003537"
                     /pseudo
     CDS             complement(5423..6493)
                     /locus_tag="OQM83_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            complement(6486..>7364)
                     /gene="flhB"
                     /locus_tag="OQM83_003538"
     CDS             complement(6486..>7364)
                     /gene="flhB"
                     /locus_tag="OQM83_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HCS1521709.1"
                     /translation="ALLPLISGVVLVALISPVMLGGLVFSGKSLQPKFSKLNPLPGIK
                     RMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLMAESPTTAMGNAMDLVGLC
                     ALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAA
                     ARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVAKGAGLVALRIREIGAENN
                     VPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHL
                     PVPEALDFINEKPTHE"
BASE COUNT         1906 a         1928 c         1761 g         1769 t
ORIGIN      
        1 catgcgggga gcggtgctgc cgatgtcacc acctgcgctc cagcggcaat accgctgtgt
       61 ttaataaatt cacgtcttgt taatgtcata acttcctccc tgatcaacga ggatcactgt
      121 ttctcggtaa tatctttggc gttgtactgg aaataccgcg ttaaaatgtc cagttcgttt
      181 tcgctcatgc tggttcgtgc ccccattcct ttggcaatgg acggccacgc attgacggtg
      241 taatggtcgg cggcaatagg ggcatgacaa ccagcgcaat aggtatcgtc aagtttttca
      301 gcgtattgcc atagcggttt acggtccgct aatccgggat cggtaagcgc accctgtaaa
      361 gacgcctgac gccattgatt gccgtattcg tcagcctgcc attccccgtt taccgtgagc
      421 gccttgatac cttcttcact taatgtggct agcgccagcc gttgacctgc cgccaggtag
      481 agcgtgtttt cactgccctg catttgataa ccctgcaaca gaacgatcgg ctgtttgcca
      541 ctggcatcaa cgacggtgag atcggtacca ggattcacgg tagccagctc gcctatgcga
      601 gaagttttga aaggataaat atgtgcgcca ttagtcactg aagtagcggc ctgactttcc
      661 agctcatgcg ccgcgttgtc atccattttt atttctggcg gaaaatgggc aatgccttta
      721 tgacaatcga tacaggtttc gccgcccttt tgtgctttgt tatgcatttt ctgcgcagat
      781 tcactttgcg aggcaatatc catggcatca aaagaatggc aactacggca cgttgcagag
      841 tcagttgctt ttaattcttt ccatactgtt tcggccattt cctggcgatg agtttcgaac
      901 ttatcgtcac tgtctatttt gccgctaaca aattcatgat aaatatcttt agatgctttt
      961 aatttagcaa ataaataatc catccctgac tttggaatat ggcaatctgc acattccgca
     1021 cgtatccctt tctggttcga aaagtggaca gttccctgat attcctcaaa agatttactc
     1081 atcgagtggc aggaaaggca aaatgctgta tccgacgttt tatgtaaggc tttttgcgcc
     1141 agcaataacc cgccgccacc aaccacaacc gctatcagca aaaacaataa cccaatgcgt
     1201 tttttccctc gcatagattc ctctaaataa attccagaac accggaatca ttgtataatt
     1261 aaatacacaa cgaattaacc gcttgcataa ctcggcacaa cactgcctga aataatcgat
     1321 aaagaatatg atttattaca atgtaatcat taattgctaa ggaataaccc agttgctatt
     1381 tagaatatat atttatcagt tttagcaatt taaatcccat aatcaatgtg aatataaaaa
     1441 atattattag cacaccgcta tgttcactgt cacccgctca acagatcacg gttatcgttc
     1501 gttttttata ctgctcaggg ataaaaaaag gcccctgttg aaattgcagg ggcctggtac
     1561 gagcaagcat catattgggc gacatgatgc aacggtaaaa atcatttggc ctgatggcgt
     1621 tcaatgattc ctttcatttc agcaaccgcc gccccgtcta cgatataacg cgaatactcg
     1681 tccgcgtgtt catctgatga catggacaat ccttgattac gataacgcat cggtgaggct
     1741 tgccacgctc ccgcggagct atggacttcc agcactccgg catcgaggaa gtggtgcagg
     1801 ttttctgcac ggactcctgc tccggccata atgattggag catcacgatg ggcaataagt
     1861 tccataattt ttgataaacc ttgcagtgcg tctgattttt gccctgatgt cagtacccgg
     1921 gcaatgccca attccgcaag attattgagt gtatataaag ggttagcgca catatcgaag
     1981 gcgcgatgaa aagtcactgc cagcggaccg gcagcagcca ttattttttc cattcgtggc
     2041 atatcgacat tcccgtcaac atcgagaacg cccgtcacca gtccaggaaa acctaattcg
     2101 cggaccgtgc gcacatcctc aagaatggcg gcaaactcac cgtcgctgta gcaaaaatca
     2161 ccaccgcgtg ggcgaattat cggatgcaca gggatcgtca cccgctggcg cacggatttc
     2221 agtaccccca acgacggcgt taagcccccc tcttttgggg ctgcgcataa ttcaactctg
     2281 tctgcgccgt tttgctgcgc cgttagtgca cattccatgc tgtaacagca aatttccagt
     2341 aatgccattt ttgctcctta attacgctga ctgctcgctg gcaacgatct cttcaatgga
     2401 ccacggatga aacttaatag ttgttttgcc atcggtgacc gccagcgtcg gattcggtaa
     2461 tcgctcatgc tcaccttttg gtgaactggt tttaactgag atccccggta ggctgagtcc
     2521 ttcatcagaa agcaatgcca gcgcacgggc attcagcgtt tctggatcac ccagcagaac
     2581 gatttcaata tgttcccaac cttcgtgtgg gtaacgtttt tccccgggcc acggtagctc
     2641 cacaatagaa aactgccagt gcgcaacctg taccggttca tgcaatttaa acagacaaat
     2701 cggtctgcca ttgatcatat tttctgacaa aagctcgcca cactgttcaa acccgcgacg
     2761 ccagcgttca gcagtggcgt tttgatggca acgcaaagaa atgtgatcgg cagtcagcgg
     2821 agtgatattc aaccccagac ggagggaaag ttcatctaat gcgtgggtaa atcgcggtaa
     2881 atccgatgca atatcctgca gctcgtcgat agattgccag ttcgccataa tcactcttcg
     2941 tctttcagta aaagcgttaa tttaccctgt tgccctgcgc caaccaaccg ctgatttcac
     3001 gccgcttctg atgcaatagt gaaaacggca atacgccacg cgcacgttgc tgacgaaaac
     3061 agccatttgc agtatactcc cgccctaatt tctttaactg gtgcgggcaa tttttgctcg
     3121 cttcatcaat gtaaggtatt ccggtgaata ttcaggctct tctctcagaa aaagtccgtc
     3181 aggccatgat tgcggcaggc gcgcctgcgg attgcgaacc gcaggttcgt cattcagcaa
     3241 aagttcagtt cggcgactat caggctaacg gcatgatggc agttgctaaa aaactgggta
     3301 tgacaccgcg acaattagca gagcaggtgc tgactcatct ggatcttaac ggtatcgcca
     3361 gcaaagttga gatcgccggt ccaggcttta tcaacatttt ccttgatccg gcattcctgg
     3421 ctgaacatgt tcagcaggcg ctggcgtccg atcgtctcgg tgtttctacg ccagaaaaac
     3481 agaccattgt ggttgactac tctgcgccaa acgtggcgaa agagatgcat gtcggtcacc
     3541 tgcgctctac cattattggt gacgcagcag tgcgtactct ggagttcctc ggtcacaaag
     3601 tgattcgcgc aaaccacgtc ggcgactggg gcactcagtt cggtatgctg attgcatggc
     3661 tggaaaagca gcagcaggaa aacgccggtg atgaaatgga gctggctgac cttgaaggtt
     3721 tctaccgcga tgcgaaaaag cattacgacg aagatgaaga gtttgccgag cgtgcacgta
     3781 actacgtggt aaaactgcaa agcggtgacg aatatttccg cgagatgtgg cgcaaactgg
     3841 tcgacatcac catgacgcag aaccagatca cctacgatcg tctcaacgtg acgctgaccc
     3901 gtgatgacgt gatgggcgaa agcctctaca acccaatgct gccaggaatt gtggcggatc
     3961 tcaaagccaa aggtctggca gtagaaagcg aaggggcgac cgtcgtattc cttgatgagt
     4021 ttaaaaacaa ggaaggcgaa ccgatgggcg tgatcattca gaagaaagat ggcggctatc
     4081 tctacaccac cactgatatc gcctgtgcga aatatcgtta tgaaacactg catgccgatc
     4141 gcgtgctgta ttacatcgac tcccgtcagc atcaacacct gatgcaggca tgggcgatcg
     4201 tccgtaaagc aggctatgta ccggaatccg taccgctgga acaccacatg ttcggcatga
     4261 tgctgggtaa agacggcaaa ccgttcaaaa cccgcgcggg tggtacagtg aaactggccg
     4321 atctgctgga tgaagccctg gaacgtgcac gccgtctggt ggcagaaaag aacccggata
     4381 tgccagctga cgagctggaa aaactggcta acgcggttgg tattggtgcg gtgaaatatg
     4441 cggatctctc caaaaaccgc accacggact acatcttcga ctgggacaac atgctggcgt
     4501 ttgagggtaa taccgcgcca tacatgcagt atgcttacac gcgtgtattg tccgtgttcc
     4561 gtaaggcaga aattaacgaa gagcaactgg ctgcagctcc ggtaatcatc cgtgaagatc
     4621 gtgaagcgca actggcagct cgcctgctgc agtttgaaga aaccctcacc gtggttgccc
     4681 gtgaaggcac gccgcatgtg atgtgtgctt acctgtacga tctggctggt ctgttctctg
     4741 gcttctacga gcactgcccg atcctcagcg cagaaaacga agaagtgcgt aacagccgtc
     4801 tgaaactggc acaactgaca gcgaagacgc taaagctggg tctggatacg ctgggtattg
     4861 agacagtaga gcgtatgtaa tcgatttttc gtgagagtaa agcctgatca gggttacctg
     4921 atcaggcttt tttattgcca tctaaatgta ttctgaaaat ggacatgcca ttgttttctg
     4981 actgttggat aagaggccag aagcgtaata tccggcccca gggaaacgat aacggttgaa
     5041 tttaaggaat accgcagtgt ttaaatttct tgtattaaca ttaggcatta tctcttgcca
     5101 ggcttacgca gaagatacgg ttatagaaaa cgaccatgac atttcagcca tcaaagattg
     5161 ttggcaaaaa aattcagatg atgatactga cattaacgtg atcaaatcat gcctgcgaca
     5221 agaatacaat ctcgtcgatg cgcaattaaa taaagcctat ggtgaagctt atcgttatat
     5281 agaacaagtg ccacgcacag gtgtaaaaaa acctgatacc gaacaactta acttgcttaa
     5341 aaaatcacag cgagcctggc tggattttag ggacaaagaa tgtgaattaa tcctttcaaa
     5401 tgaggacgtt caggatttaa tgtacatcgt tccacgtcgc ttcgacaacg gtattattct
     5461 ctgccatttt taccggtttg ggttcggcag gcgctttttg ttcgcgtccg cgtatccacc
     5521 aggccagccc gagcaatccg gcagtgaaca gcaaaaatac caggttcggc attccaggca
     5581 ccaggccgag taaaccgagc acggcggcgc ttaacaacat aacgcttggg ttactgaaaa
     5641 gctgattcac catctgctcg ccaacatcct gatcggtgct gacacgcgta acgatgaccc
     5701 ccgcggcggt aaaaatcacc agcgccggaa tttgtgccac cagaccatcg ccaatggtca
     5761 atagcgtata actttccgcc gcgtgtccca tgctcatgcc atgttgcagc acgccgacca
     5821 gcaacccgcc gacaacgtta atgaccatga tgaggatccc ggcgatggca tcgccgcgaa
     5881 caaacttact tgccccgtcc attgagccgt aaaaatcggc ttcctgagtc acttcggagc
     5941 ggcgtttttt cgcctcatct tcaccaatca atccagcgtt aaggtcggcg tcaatcgcca
     6001 tctgcttacc cggcatacca tcgagaacaa agcgcgcacc cacttctgcg atacgcccgg
     6061 cacctttggt aatgaccata aagttgatga tcacgagaat gacaaacacc acgataccga
     6121 tagcgaaatt gccaccaacg aggaagtgac cgaacgcttc gaccaccttc cctgccgccg
     6181 ccgcgccggt atgcccttcc atcaaaatga tacgggttga agccacgtta agtgccagac
     6241 gcaacaacgt agtaaacaac agaatggtcg gaaacgcagc aaactcaagc gtgcgctggg
     6301 taaacatcgc caccagcaac accatgatcg acaaggcaat attgaaggta aacaacaggt
     6361 cgagtatgaa tgcgggcagt ggcagcacca tcatcgacaa gatcaacagg atcaaaatcg
     6421 gtccggcaag gatctgccat tgtgtcgatt tcaggtttgc gggcaggcgc agcatcgcgg
     6481 ccagattact catgggtcgg tttctcgtta ataaaatcca gggcttccgg caccggaaga
     6541 tgagtaggtt gtacagggcg ctgtccacca gccagacgcc agcgtttcag ttgccagacc
     6601 caggccagca cttccgccac cgcggcgtac agttgacccg ggatttgttg accaatctcc
     6661 gcatgtcgat acagcgctcg cgccagcggc ggcgcttcaa gcgtcgggac gttattttca
     6721 gcgccaattt cacgaatgcg cagcgcgacc agccctgcac ctttagcgac cactttcggt
     6781 gcgctcattt tgttttcgtc atactgcaac gctaccgaat agtgggtcgg gttattgaca
     6841 atgacatcag ctttcggcac atcggccatc atccgacgcc gtgcagcagc tcgctgcatc
     6901 tgacggatcc gccctttaac atgagggtca ccttcgcttt gtttgaactc atcacgaata
     6961 tcctgccgcg acatacgcag ctttttcagg tggctgaaga tttggaaaaa gacgtcaaat
     7021 cccaccattg gaatgacacc aagcaccacc agcagtgcgc atagcccaac cagatccatc
     7081 gcattaccca tggcggtagt cggagactcg gccattaagc gcatcatctg cggccagtga
     7141 tgccagagat aaaaccccgt cacgctgcca accaggatgg ttttcaaaat tgccttaagc
     7201 aactccgcgc cagtctgtgc cgagaacatc cgtttaatgc ccggtagcgg gttgagtttg
     7261 gaaaacttcg gctgcaagga tttgccgcta aataccagcc cccccagcat gaccggagaa
     7321 atgagcgcca ccagcaccac gccgctaatc agcggcagca gcgc
//
LOCUS       DAJVQG010000175         7323 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJVQG010000175 DAJVQG010000000
VERSION     DAJVQG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7323
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.175"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..935
                     /gene="rpoS"
                     /locus_tag="OQM83_003539"
     CDS             <1..935
                     /gene="rpoS"
                     /locus_tag="OQM83_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCS1521710.1"
                     /translation="GVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGE
                     IGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEE
                     GNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVYLR
                     TARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDIL
                     ADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIG
                     LTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(1029..2393)
                     /locus_tag="OQM83_003540"
     CDS             complement(1029..2393)
                     /locus_tag="OQM83_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HCS1521711.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALCASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(2482..3258)
                     /locus_tag="OQM83_003541"
     CDS             complement(2482..3258)
                     /locus_tag="OQM83_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCS1521712.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGYEYKPRGLTEEGLGWFDAWR"
     gene            complement(3263..3901)
                     /locus_tag="OQM83_003542"
     CDS             complement(3263..3901)
                     /locus_tag="OQM83_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCS1521713.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(3898..5160)
                     /locus_tag="OQM83_003543"
     CDS             complement(3898..5160)
                     /locus_tag="OQM83_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCS1521714.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(5157..6065)
                     /locus_tag="OQM83_003544"
     CDS             complement(5157..6065)
                     /locus_tag="OQM83_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS1521715.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            6261..7028
                     /locus_tag="OQM83_003545"
     CDS             6261..7028
                     /locus_tag="OQM83_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1521716.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(7079..>7323)
                     /locus_tag="OQM83_003546"
     CDS             complement(7079..>7323)
                     /locus_tag="OQM83_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCS1521717.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
BASE COUNT         1831 a         1893 c         1899 g         1700 t
ORIGIN      
        1 acggagttga ggtttttgac gaaaaggcct tagtagaaga ggaacccagt gataacgatt
       61 tggccgaaga ggaactgtta tcgcagggag ccacacagcg tgtgttggac gcgactcagc
      121 tttaccttgg tgagattggt tattcaccac tgttaacggc cgaagaagaa gtttattttg
      181 cgcgtcgcgc actgcgtgga gatgtcgcct ctcgccgccg gatgatcgag agtaacttgc
      241 gtctggtggt aaaaattgcc cgccgttatg gcaatcgtgg tctggcgttg ctggacctta
      301 tcgaagaggg caacctgggg ctgatccgcg cggtagagaa gtttgacccg gaacgtggtt
      361 tccgcttctc aacatacgca acttggtgga ttcgccagac gattgaacgg gcgattatga
      421 accaaacccg tactattcgt ttgccgattc acatcgtaaa ggagctgaac gtttacctgc
      481 gaaccgcacg tgagttgtcc cataagctgg accatgaacc aagtgcggaa gagatcgcag
      541 agcaactgga taagccagtt gatgacgtca gccgtatgct tcgtcttaac gagcgcatta
      601 cctcggtaga caccccgctg ggtggtgatt ccgaaaaagc gttgctggac atcctggccg
      661 atgaaaaaga gaacggtccg gaagatacca cgcaagatga cgatatgaag cagagcatcg
      721 tcaaatggct gttcgagctg aacgccaaac agcgtgaagt actggcacgt cgattcggtt
      781 tgctggggta cgaagcggca acactggaag atgtaggtcg tgaaattggc ctcacccgtg
      841 aacgtgttcg ccagattcag gttgaaggcc tgcgccgttt gcgtgaaatc ctgcaaacgc
      901 aggggctgaa tatcgaagcg ctgttccgcg agtaagtaag catctgtcag aaaggccagt
      961 ctcaagcgag gctggccttt tctgtgcaca ataaaaggtc cgatgctcat cggacctttt
     1021 tattaaggtc aaattaccgc ccatacgcac caggtaatta agaatccggt aaaaccgaga
     1081 atggtcgtta acactgtcca ggttttcaga ccgtctgcta ccgacaaccc cagatatttg
     1141 gtcacaatcc agaaccctga gtcattaata tgtgacgccc caagcccacc aaagcaggct
     1201 gccagcgtca ccaatacgca ctgaatcgga ttcaatccca tcaccgcttc tgagagtaac
     1261 ccgccggttg tcaggattgc tacggttgct gacccctgcg atgcacacag cgccagtgaa
     1321 ataataaatg cggctggtaa cagaggcagg tcaatcattt gtagcatgtt ggcaagggct
     1381 ttgccgacgc ccgattccac cagcactttg ccaaataccc ctccagcacc agtaaccaaa
     1441 atcactaccg ccgcagtggg aagggctgag cccataatgt cgctggtgtg ttgtaagctc
     1501 cagccgcgac gtaaagccaa taaccagaat gccagcacca gcgcaatcat tagagctacc
     1561 attggtgagc cgatcagctg tagcgtacca agcaggggat gcgaaggcgg catcagtgtt
     1621 gcggaaaccg tacccgccat gataatcgcg ataggaataa caattagcga ggtgaccagc
     1681 gcgacgcccg gtggatttat tttatcgctt aattttgtcg cgccttcctc actggccgga
     1741 gccagttgca tctgttccag tacttccacc gacatcgcat attggtgctt attgattatt
     1801 ttcgctgcaa agtagccaac aatccctacg ggaatagaaa tcgcaatacc gatgatggtt
     1861 agccagccga tgtctgcgtg gagtaacccc gctgcggcga cagggcctgg atgcggcggt
     1921 accgccacat gtacagttag catgatccca gcgacaggca ggccaaattt gagtggcgat
     1981 attttggcaa ccttggcaaa accgtaaatg attggagcaa gaataataaa gccgacatca
     2041 aagaagacgg gaataccgag gaagaacgct gccagagtca gcgcagcgat agttcgtttg
     2101 tcacctaact tgcgactgaa ataattagcc agtgactctg caccaccaga gtgttcgatc
     2161 atacgcccca gcatagcgcc cagaccaata ataatagtga cggaaccaag cacaccgccc
     2221 atcccggcga tcatcacttt acccacttcg cccgccggta tacccgccgc aagtgcgact
     2281 aacaggctga cgaggagcag agcaacgaat ggttgtacct ttgccttgat gaccagcagc
     2341 aacagcatga ttacgccagc taacgcaatg cataacaatg taattgtgga catgggaaac
     2401 cctgtctgaa agttatagtt aacctacccc atccgtagat ggggggatgt atgggtacgt
     2461 tgtaattagg gatttaacga attagcgcca ggcgtcaaac cagccaagcc cttcttcggt
     2521 gaggccacga ggtttatatt cataaccgat ccagccctga tatcccacct catcgaacag
     2581 gcggaacagc cacggatagt tgatttctcc atcgtccggt tcatgtcgat caggtagtcc
     2641 ggcaatttgt acgtgcgcat atttcccagc gtagtcgcgg attaaatgcg tcaggttgcc
     2701 atctactttt tgcgcatgaa aagtatctag ttgaataaac acgttatctc gcgcaacctc
     2761 ttcaacaata gccagtgcct gatactggct ggagaagaga taatgaggct taacgtcggg
     2821 gctgagtgct tcaactaata ttcgcttgcc gtgtagcgca aagcggtcgg cagcgtagcg
     2881 gagattatcg ataaatactg cccggtaccg ttcagcgtct tcgccagcgg gcacgacgcc
     2941 tgccatcaca tggacttgtt cacaattgag cgccaatgca tattccagtg ccaggtcgat
     3001 gtctgcgtgt gcttcgtgct cacgtccggg aagggcggat aatccccatt cccccgcatt
     3061 aatatctccg ggagcggtat tgaacagcgc cagtgtcaga tggttttgct ccagttgctt
     3121 ttggatttgc agggtggagt agtcataggg aaacagaaat tccacagcat cgaacccggc
     3181 ttttcgcgct gcggcgaagc gttcaataaa aggcacttcg gtgaacatca tggataaatt
     3241 agctgcaaaa cgaggcattg cattaactcc ttaattccgc aatttcacct gcggtcagat
     3301 aacggatcgg gcggtcaccg agaataaaaa tcagctttgc cgtttcctcc agctcttcca
     3361 tattgttggc ggcttcttgc aggctttcac cgcaaaccac tgggccatga tttgccagta
     3421 aaaaagcctg attgtctgct gccagttccg ccagatcctg tgcgatgcgt ttatcgcccg
     3481 gtcggtaata aggcaccagc gggacatttc ccatccgcat caccacgtat ggtgtgaacg
     3541 gacgaataac gttgctgctg tccagccctt gcaggcagga aagcgccgtc gaccatgtgc
     3601 tgtgcaaatg caccaccgct ttacagcgcg gattgttgcg atacagcgcc agatgaaaga
     3661 gcacctcttt cgagggtttg tcaccactta accattcgcc atccgcggcg actttggaaa
     3721 gccgctgcgg atcgagattg cccaggcatg aacctgtcgg tgtcgccagt aaattcccgt
     3781 caggtaaaag cagcgacaga ttgccagccg aaccggttgc atagccgcgc tgaaagaatg
     3841 aactggcaat ccgcgtcatc tcctctcgca aagactgctc tacttttgcg aaatcgctca
     3901 tgataaaaac tctctttggg ctcgtgaaaa aaaggcttca tcaccgaagt tgccagattt
     3961 aagggcgagt gagacaggct tatccagtgc gtttacccac ggcacgccgg gggaaatggt
     4021 tgggccaata tgaaaccctt ttatgcccag gctctgtgtg actacgccgg aggtctcacc
     4081 gcctgcgaca ataaagcgtg tcacgccttc cgctgctaac cgcgccgcta gttgagaaaa
     4141 cagagtttct actgcctgac tggctttttg tgcaccgtat tgctgttgaa ttgctgccaa
     4201 tgcgtcagtg ctggcggtgg caaaaaccag tggagcaagt acactttcct ggcccagaac
     4261 ccactctgcc agttcgtgtg cataagcggc cagagtttcg gttgagaggc agcgtgccac
     4321 atcaacttca cgggctggtg caatttgacg gtaatgtgcc acctggcggt tggtcatttg
     4381 agagcatgaa ccggagagca ctacgccgcg cccagcgagc ggacgccctg cttcgcgagc
     4441 ctggttaccg ttttcttgcg cccactgccg ggccaggcca atcgccagac cagaaccgcc
     4501 cgttaccagt ggggcatcgc gcaaggcttc tccctgaatt tccagatggt gttcggtcag
     4561 cgcatcaagc accgcgtagc ggtagccctc ttgctgtaag cgagccagct cttgacgaac
     4621 ggcatccacg ccttgttcga aaacatgtgc cgaaacgacg ccgcagcgcc ctgtggattg
     4681 cgcttcaacc agacggggaa gatagctgtc ggtcatggga tttaccgggt gatggcgcat
     4741 cccggattcg gccagcagtt gattcattac gaacaaatac ccctgataaa ccgtacgtcc
     4801 gttgaccgac agggccggag agaagaccgt aaacggcgtg tcgagagcat ccattaaggc
     4861 atcggtaacc gggccaatat tacctttcgc cgtactgtcg aaagtagagc agtatttgaa
     4921 atagatctgt ttgcaacctt gctgttgcaa ccagctcaga gccgccagcg attgctgtgt
     4981 ggcttcaacc accggacagg agcgcgtttt caggctgatc accagtgcgt cgattgcttc
     5041 cggcatttta cctgttggaa caccgttaat ttgtaccgtt ggtagaccgt tttccaccag
     5101 aaaactggcg atatccgtcg cgccggtaaa atcatcggcg ataacgccaa tcttgatcat
     5161 gatttcgctc ccggtagagt gatgccagag aaaatcttga taactgcgct atcgtcttct
     5221 ttcccgtaac ccgcgttact ggcgctggtg aacatattca atgctgttga ggccaatggc
     5281 agcgggaagt gcagggcttt ggctgtatcg gcaaccagac caagatcctt aacaaaaata
     5341 tcgacggctg aatgcggggt gtaatcgcca tccaccacat gacgcatccg gttttcgaac
     5401 atccaggaat ttccggcggc attggtcacg acgtcataca tcacatccag cgggatcccc
     5461 gcacgggctg caagtgccat cgcttcggct ccggcagcaa tatgtacgcc cgctaacaac
     5521 tggtgaataa tttttacggt cgaacctagt cccggttctg cacctatgcg ataaactttt
     5581 ccggcaacgg cttccagcac gggtgccagt cgttcaaagg caatatcgct accggaggcc
     5641 atgacagtca tttcaccgtt agcggctttt actgcaccac cagaaactgg cgcatccagc
     5701 atttccagat cgaatccagc cagagcggta gcaatttctt gcgcatcagc actagcgata
     5761 gtggaagaaa ccattactgc cgtaccgggt ttcagatgtt gtgcaacgcc tgtttcacca
     5821 aacagcacct gtttaacctg ggccgcattg gccaccagca ccagcagagc gtccagtttt
     5881 tcggcaaacg tcgcggcgtt atcagaaacc ccgcaagcac ctgcctcttt caacgtagcg
     5941 caggcattgc tgttcaggtc tgcgccccag gtagaaagac ctgcgcggac acatgacagt
     6001 gctgctccca ttcccattga ccctaagcca acgataccga catgaaactc agatcccgtt
     6061 ttcatctgct ctcattgtta atttaagtga tattttgttt gatattgtga atataagcgc
     6121 tggaagataa cgatatggtg agctgattca cataaattaa cattgtgtgt tattttatgt
     6181 gaactaagcg ttagttgcgc cgcgcacgtt tcgcaggcaa atagcgtaga atgtcagcag
     6241 gacaaaggaa ggagcaaaag ttgatacccg tagagcgtcg ccaaatcatc cttgagatgg
     6301 tagctgaaaa aggcattgtc agtattgctg aactaacgga cagaatgaat gtgtcacata
     6361 tgaccattcg tcgggattta caaaaactgg agcagcaggg agccgttgtg ctggtgtccg
     6421 gaggcgtcca gtctccggga cgcgtggcgc atgaaccttc tcatcaggta aaaactgcgc
     6481 tggcaatgac gcaaaaagcg gctattggca agctggcggc aagtcttgtt cagccgggaa
     6541 gttgtatcta tctggatgcg ggaacgacca cgttagcgat agcacagcat ctgattcaca
     6601 tggagtcact gactgtggtc acaaacgatt tcgttattgc gaactacttg ctcgacaaca
     6661 gtaattgcac aattattcac actggcggtg cagtgtgtcg ggaaaaccgt tcctgtgtcg
     6721 gggaagccgc tgcgaccatg ctgcgcagcc tgatgattga tcaggctttt atttctgcat
     6781 cgtcatggag tgtgcggggg atttctacgc cagcggaaga taaagtcacg gtgaaacggg
     6841 cgattgccag tgccagccgc cagcgagttt tggtctgtga tgcgacgaaa tatggtcagg
     6901 tggcgacatg gctggcgtta ccattaagcg agtttgatca gattatcaca gacgacggtt
     6961 tgccggagag tgccagtcgc gcgctggcga agcaggatct ctctttgctg gtagcgaaaa
     7021 atgaataatg gcctgcaata acatttggtt actcatgctt cacagaagaa gcatgagact
     7081 actttatttt ataaaatgac agccgcccgc ttttcggcgt gccggtatca atataaatct
     7141 ggttagcgaa cgtctgaatg ttatcaaaca tcatatgtcc aaatataaaa taatcagcgc
     7201 cgtttatttg ttgtaactcg ccattaagcg atttctgcac acgatcaaca ggccagagta
     7261 attcgctttc tgctatttct ttaccaaact gatattcatc acccggataa tctgcatgtg
     7321 cga
//
LOCUS       DAJVQG010000176         7143 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJVQG010000176 DAJVQG010000000
VERSION     DAJVQG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7143
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.176"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..2217)
                     /gene="ppsA"
                     /locus_tag="OQM83_003547"
     CDS             complement(<1..2217)
                     /gene="ppsA"
                     /locus_tag="OQM83_003547"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCS1521718.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIR"
     gene            2550..3383
                     /gene="ppsR"
                     /locus_tag="OQM83_003548"
     CDS             2550..3383
                     /gene="ppsR"
                     /locus_tag="OQM83_003548"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCS1521719.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            3540..4586
                     /gene="aroH"
                     /locus_tag="OQM83_003549"
     CDS             3540..4586
                     /gene="aroH"
                     /locus_tag="OQM83_003549"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCS1521720.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            4718..4909
                     /gene="hemP"
                     /locus_tag="OQM83_003550"
     CDS             4718..4909
                     /gene="hemP"
                     /locus_tag="OQM83_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCS1521721.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(4913..6349)
                     /locus_tag="OQM83_003551"
     CDS             complement(4913..6349)
                     /locus_tag="OQM83_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCS1521722.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(6412..7125)
                     /gene="ydiV"
                     /locus_tag="OQM83_003552"
     CDS             complement(6412..7125)
                     /gene="ydiV"
                     /locus_tag="OQM83_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCS1521723.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         1810 a         1915 c         1707 g         1711 t
ORIGIN      
        1 acggatagcc atcgacagca gtgctttcac cgcatcgttg cgctcatcga acaattcaga
       61 caccacgccg gagtcacggt ccagaccgag cgccagctgc gtcatatcgt ttgagccaat
      121 tgagaagccg tcgaaatatt cgaggaactg ctcggccagc aaggcgttgg acgggatttc
      181 acacatcatg atgattttca gcccgttctc accacgcttc agcccctgac gcgccagttc
      241 ctcaaccacc gctttcgcct gatctacggt acgcacgaac gggatcatga tctcaacgtt
      301 ggtcagcccc atgtcgttgc gcacacgttt cactgcttcg cactccagcg cgaagcagtc
      361 gcggaagctg tcggaaacat agcgtcccgc gccacggaag ccgagcatcg ggttctcttc
      421 atctggctcg taacgctcac caccgaccag gttggcatat tcgttcgatt taaaatcaga
      481 gagacggaca atgacgcgct tcggataaaa cgcggcaccc agcgtcgcga tcccttcagt
      541 cagacgacca acgtaaaatt cacgcggaga atcaaaacct ttcatcatct cgcggatttc
      601 attttgcaac tgcggttcct gatcgtcaaa ctcaagcagt gcgcgtgggt ggacgccaat
      661 catacggttg atgataaatt ccagacgcgc aaggcccacg ccttcgttcg gcaggcaggc
      721 gaagtcgaaa gcacggtccg ggttaccgac gttcatcatc actttcaacg gcagatccgg
      781 catcatttct acgctggagc ttttcacgct aaattccagc aactccgcat agacgtaacc
      841 ggtatcacct tcggcacaag aaacagtgac gttctcaccg tctttcatcc gttctgttgc
      901 atcaccacag cccactaccg ccggaatgcc cagttcacga gcgatgatcg ccgcgtgaca
      961 ggtacgaccg ccacggttgg tgacgatggc agatgctttc ttcatgatcg gttcccagtc
     1021 cgggtcggtc atgtcagtga ccagcacgtc accaggttcg atgcggttca tttcgctgat
     1081 ctcatggatg actttcaccg gacccgcgcc gatgcgatga ccgatagcac ggccttcggc
     1141 gataatctta ccctgtgaat gcagcgtata acgctccatg acctgaccgc gtgagcgcac
     1201 ggtttccgga cgcgcctgca caatgaacag tttgccagtg tggccatctt tcgcccactc
     1261 aatatccatc gggcgaccgt agtgtttctc aatttgtacg gcctgttttg ccagttcctg
     1321 cacttcttcg ttggtcagcg agaagatgtc acgctgttcc tgcggtacgt cttcgatttt
     1381 aacctgcttg ccgtgctcct gggtcggcgc gtaaaccatg cggatttttt tcgaccccat
     1441 ggtgcgacgc acgatagccg ggcgattcgc cgccagtgtc ggtttatgga cgtaaaactc
     1501 atccgggtta accgcaccct gcacgaccat ttccccaagg ccccatgcgg aagtgataaa
     1561 caccacctgg tcaaagccgg attcggtatc aatggagaac atcacgccag aagatgcgag
     1621 gtcggagcgc accatccgct gaacaccggc agagagcgcc acgccacggt gatcgtaacc
     1681 ctggtgcaca cgataagaga tggcgcgatc gttaaacaga gaagcaaata catgcttcac
     1741 tgccacgaga acggcgtcaa aaccctgaac gttgaggaag gtttcctgct gaccggcaaa
     1801 agaagcgtcc ggcatatctt ctgcggtggc ggaggagcgc accgcaaaag aggcgttttc
     1861 gtcatcggcg gaaagctgtg cataggcttc gcggatggcg ttttccagct caggctggaa
     1921 gggagtgtcg ataatccact ggcggatttg cgcgcccgct ttcgcaagct gagtaacatc
     1981 gtcaatatcc gttttatcca gcagttcata aatgcgctgg tttacgccgc tttggtccag
     2041 aaactggtta aacgcgtcgg cggttgtggc gaaaccattc ggaacggaaa cacccattcc
     2101 ggaaaggtta gtaatcattt cacccaggga ggcatttttg cccccaaccc tgtctacatc
     2161 attcatgccg agttggttat accaaagcac cagcggtgac gagccattgt tggacatcga
     2221 acaatccttt tgtgataaat gaacggtttg agaaacacat ttctgcgcat ttatctttgc
     2281 atatttaacc ggatgaaaaa aacggtgaat cgttcaagca aatatatttt tttacttttt
     2341 aagactgatc ccagcgttgc gcaaatctgc tctcttcgac aatttccaca taaaccatcg
     2401 gtataaacga aaccataaaa atgaaatgct gttttcataa aaaataaaat tgaaggttca
     2461 ttttataaac cagacataac gtcctacgct tctgtgcgtt tttaatttat gctttcatag
     2521 aattatgtct gcatcacggg aagaacaaaa tggataatgc tgttgatcgc cacgtttttt
     2581 atatttctga tggtacggca ataactgcgg aggtattagg acacgcagta atgtcacaat
     2641 ttcccgtcac tatcagcagc attacgctgc cgtttgtcga aaatgagagc cgtgcacggg
     2701 cagtgaagga tcagattgac gcaatttatc accagacagg tgtgcgcccg ctggtcttct
     2761 actccatcgt gttgccggag attcgcgcca tcatcttgca aagtgaaggc ttttgccagg
     2821 atatcgttca ggcgctggtt gccccgctac aacaagagat gaaactggat ccaacgccga
     2881 ttgctcatcg tacccatggc cttaacccta ataatctcaa taaatatgat gcgcgcattg
     2941 cggcgattga ttacaccctc gcccacgatg acggcatttc gttgcgcaat ctggatcagg
     3001 ctcaggtgat cctgctcggt gtttctcgct gtggtaaaac ccccaccagt ctttatctgg
     3061 caatgcaatt tggtatccgc gcggcaaact acccctttat tgccgacgat atggataatc
     3121 tggtgctacc cgcgtcgctc aaaccgcttc agcataaatt gttcggcctg actatcgacc
     3181 cggaacgtct ggcggcgatt cgcgaggaac gtcgggagaa cagtcgctat gcctcgcttc
     3241 gtcagtgcag gatggaagtc gcggaagtgg aagcgttgta ccgtaaaaat cagatcccgt
     3301 ggattaacag taccaattat tcggtagaag agattgccac caagatcctc gatatcatgg
     3361 gccttagtcg ccgaatgtac tagagaacta gtgcattagc ttattttttt gttatgatgc
     3421 taaccacccg gcgaggtgtg acacacctcg cacttgaaat cagcagcgat tggtttatcg
     3481 tgatgcgcat cacttcccgg cagtcctgcc gtagaagcaa caaatttctg agacttgtaa
     3541 tgaacagaac tgacgaactc cgtactgcgc gtattgagag cctggtaacg cccgccgaac
     3601 tcgcgctacg gtatcccgta acgcctggcg ccgccaccca tgtcaccgac tcccgccgca
     3661 gaattgaaaa aatactcaat ggtgaagata agcgactgtt ggtcattatt ggcccctgct
     3721 cgatccacga tctcaccgct gcaatggagt acgccccccg tctgcagtcg ctgcgcaacc
     3781 agtaccagtc acggctggaa atcgtaatgc gcacctattt tgaaaaacca cgaactgttg
     3841 tcggctggaa aggactaatc tccgatccag atttaaacgg cagctatcgg gtaaatcacg
     3901 gtctggagct ggcgcgcaaa ttacttttac aggtaaatga gctgggcgtc ccaaccgcga
     3961 ctgagttcct cgatatggtg accggtcagt ttattgctga tttaatcagt tggggcgcga
     4021 ttggcgcacg tactaccgaa agtcagatcc accgtgaaat ggcttcggca ctctcctgtc
     4081 cggtaggttt taaaaatggt accgatggca atacgcggat tgctgtggat gctatccgcg
     4141 cagcccgcgc cagccatatg ttcctctcgc cagacaaaaa tggtcagatg accatctatc
     4201 agaccagcgg caacccgtat ggccacatta ttatgcgtgg cggcaaaaaa ccgaattatc
     4261 atgccgatga tatcgccgca gcctgcgata cgctgcacga gtttgattta cctgaacatc
     4321 tggtggtgga tttcagccac ggtaactgcc agaagcagca ccgtcgccag ttagaagttt
     4381 gtgaggatat ttgtcagcaa atccgcaatg gctctacggc gattgctgga attatggcgg
     4441 aaagtttcct gctcgaagga acgcaaaaaa tcgtcggcag tcagccgctc acttacggtc
     4501 aatccattac cgacccgtgt ctgggctggg aagataccga acgcctggtc gaaaaactcg
     4561 cctctgcggt agatacccgc ttctgaatgc gtgcccattc ctgacggaat gggcatttct
     4621 gcgcaacttg ttgtcttctc aacaaattac tgcttgctct ggcctgccat aatattgata
     4681 ataagaatca ttgttatatc aattattatt aatctttatg cgttatacgg atagcagaaa
     4741 actcacgcct gaaacggacg ccaatcacaa ggccgcttcc ccgcagccca tccggcgaat
     4801 taccagccag acactgttag gtccggatgg caaactgatt atcgatcatg acgggcaaga
     4861 atatctgctc cgtaaaactc aggctggcaa gctgctgtta actaagtagc ctttaactcg
     4921 agcagctgac ttccagccgt ttaccccagt caggtggacg actgacatag tcatcatctc
     4981 tatcgctgaa aggatttcgc aacgccccat gcaggcggtg caattccgtc atatcaccct
     5041 tttctgccgc ttcaatcgcc cgttgcgcca accagttgcg caataccaga gcggggttaa
     5101 cgctttgcat caactgctga cgctcactat cgctaacctc gtcttgttgc aaacgccccc
     5161 gataacgggc aaaccagcca tcaaatgccg cacgatcaat aaactcatca cgtagcggtg
     5221 acgccgtgct gtgctgctcg gtcagactca gcatgcggaa tgtgcgggta taatcgctgc
     5281 gctctcgcgc catcagactg aataattcat tcagtagcgc gttatcctct ttttgctccg
     5341 tgatgaagcc cagtttctgc cgcatccgtt gtccataatg cgtcaacaaa acctgctgat
     5401 agctgtccag ggcctcattc agggcatcta cggcaacaaa tggtgacaat gtctgcgcca
     5461 gacgctgtaa attccacaac gcgacagcag gttgattatc aaaactataa cgcccttgat
     5521 gatccgagtg attacaaata aaaccgggtt cgtaatcatc aagaaaacca aacggcccgt
     5581 aatcaagcgt cagtcccagc agcgacatgt tgtcggtatt catcacccca tgtgcaaagc
     5641 cgaccgtctg ccattgggca attaacgacg cggtacgtgc gacaacatcg ctaaaccaga
     5701 gacggtattt gtcctcatca tcttcaagat gtgaccagta atgacgaata gcaaagtcag
     5761 ctaactgacg aaccttttcc gactcgcggc ggtagtaaaa atgttcgaaa tgaccaaagc
     5821 gcagatgact tggtgccaca cgcatcagca tcgcgcctgg ctccgccgtt tcccgataca
     5881 ctggcgaatc gctggtgacg atacttaacg cgcgggtcgt cggaatgccc agataatgca
     5941 tcgcctcact ggcgagactt tctcgtatcg tcgaacgtaa aaccgcccgt ccatcaccca
     6001 ttcgcgaata aggcgtcagg ccagcacctt tcagatgcca gtccattgta gtgccatcag
     6061 caagcagttg ttcgccgagt aaaatgccgc gcccatcacc cagttggccc gcccagacgc
     6121 cgaactgatg accactgtaa acctgcgcca gtggtgacat accaggcagt agggcttcgc
     6181 cgccccagac acctgcgcca tttttaaaca gcgacgatgg aatactcagc gtgttagcca
     6241 gttcggtatt atgccaaatc agccgggcat tatttaaagg cgtaggggaa agtgctgtat
     6301 aggtttctgg caattcatcg cgccagcggg taacaaaaga cagggtcata gatcctcctg
     6361 tttgatagtg tagacggtta ctctcgtctt aaacaccagc aaacagaagg gttatcgctg
     6421 aaccaacgtc gttatctgat ttatgggaac ggcaggccac aggcatccct ggagagcatg
     6481 gaaatcgaag ggggtaatct gtgccaatat ttctgccgta tcaatgcctc ccgcaatgat
     6541 gcaattgcaa caaggggaaa tttgcgcctg gatcgcacga ataaacggtt cgaaggagcg
     6601 gtgcgtgatt tgttgctgaa taaaactttt atccagcatg acgcgggtga aaagaccatc
     6661 aaaaaccgct ttcattgtgc tattgcctgc tcccaaatta cctaacacaa ggggatacac
     6721 ttgcgataag gataaaagac cgcgattatc ttttccttca ttgagatgcg ggtaattctc
     6781 attaatcaac agttcaataa acggatattt taatagttcg cctgcgtaat tatccctctc
     6841 taataataat gtagccactt gtggcgttaa attaagccag gcaaagagtt tatgttgaat
     6901 aaaaaaatgt tggcatgatt ttagtaattc caactgctct gaaaataatt gccaatgttg
     6961 ctcctcggta agttgcgcta taacccgact cgtcgggata cgcaccgtgc catcttcact
     7021 ggagaaatgg gtaattaact caacgccgac aaggacttgc tggttatctc ttatcggcag
     7081 gaaatagcaa tcagaatgat aaagattctc caaaaaaatc ttcatggtaa ccgtccctct
     7141 cga
//
LOCUS       DAJVQG010000177         7121 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJVQG010000177 DAJVQG010000000
VERSION     DAJVQG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.177"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            4..435
                     /locus_tag="OQM83_003553"
     CDS             4..435
                     /locus_tag="OQM83_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCS1521724.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQFA
                     VQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            518..1420
                     /locus_tag="OQM83_003554"
     CDS             518..1420
                     /locus_tag="OQM83_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCS1521725.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="OQM83_003555"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="OQM83_003555"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCS1521726.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="OQM83_003556"
     CDS             complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="OQM83_003556"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCS1521727.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="OQM83_003557"
     CDS             complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="OQM83_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCS1521728.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5330..6037)
                     /locus_tag="OQM83_003558"
     CDS             complement(5330..6037)
                     /locus_tag="OQM83_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCS1521729.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="OQM83_003559"
     CDS             complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="OQM83_003559"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCS1521730.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6895..7098)
                     /locus_tag="OQM83_003560"
     CDS             complement(6895..7098)
                     /locus_tag="OQM83_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1521731.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1640 a         1991 c         1853 g         1637 t
ORIGIN      
        1 aaggtggcgt taaatctcgc ttcaggtgaa gggaatttct ttatccgtcc tggcggcgtg
       61 ttttatgtcg cgggagataa agtcgacatc gttcgtctgg atgccttcaa aaccagtaaa
      121 gagattcagt ttgcggtgca gtcagggcca atgttgatgg aaaacagtgt aattaatccg
      181 cgtattcatc ccaacgtcgc ctcacgtaaa attcgtaacg gtgttgggat taataaacat
      241 gggaatgccg tgtttttgtt gagccagcag gcgacaaatt tttatgattt tgcctgttat
      301 gccaaagcga aactgaacgt tgagcagctg ctttatcttg atggcacgat ttcacatatg
      361 tatatgaaag gcggggcaat cccgtggcaa cgctatcctt ttgtcaccat gatttccgtc
      421 gagaggaaag gttaagcatt gtacactcga ggaggccaga caaactgggg ctggctacac
      481 agctgaaaaa tcatcagata aatgaaggga aacaaaaatg aaaaaaacgc tcgcaacgtt
      541 atttttatta acgtgtctgg gatcatcatc cgcttatgcg gataacgctt tgattttaca
      601 aactgatttt agcctgaaag atggtgccgt ctcggcgatg aaaggggtcg cattcggtgt
      661 tgaccataat cttaaaatat ttgatttaac acacgaaatt ccaccgtata acatctggga
      721 aggcgcttac cgcctgtatc agaccgccag ttactggccg caaggttcgg tatttgtcag
      781 tgtggtcgat cctggtgttg gtacagaccg taaatcggta gtgctaaaaa ccaaaaacgg
      841 tcaatacttc gtttcaccag ataacggtac gctcactttg gtggcggaat cgctgggtat
      901 tgagagcgtc cggcaaatcg atgaaaaaac caaccgcctg aaaggttccg aaaagtcata
      961 taccttccac ggtcgtgacg tgtatgccta caccggtgca cgcctggctt ccggagcgat
     1021 cacgttcgaa caggtggggc cagagcttcc tgcaaaagtt gtcgaacttt cttatcagaa
     1081 agccaaagca acaaaaggtg aagtgaaggg caatatcccg attctcgata ttcagtacgg
     1141 caacgtctgg agcaatatca gcgacgagtt gctgaatcag gcaggaatca aactcaacga
     1201 cacgttgtgt gtaaccattt ccgaaggttc ccagcaaaaa tacgcaggga aaatgccgta
     1261 tgtcgcaagc ttcggcgatg tgccggaagg ccagccgatg gtgtatctaa acagcttatt
     1321 gaatgtttcc gtggcgctca atatggataa tttcgcgcag aaacatcaag ttgcgtctgg
     1381 tgctgactgg aatattgatg tgaagaagtg cgctaagtaa ggtgctgatg caggatgcgg
     1441 tgtggcgcac cgcatcctgc aacggagttg taaattacac cgtctccagc cgggcgtaag
     1501 ccgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg ctatgttcac
     1561 cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgc acgcgctggc
     1621 cgagtttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga tggctaaccg
     1681 ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc agctcgccga
     1741 taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt tccgcgtagg
     1801 tcagcgtcag tttctgcatc gcacgggtta cgccaacgta ggccagacgg cgttcttctt
     1861 ccagacgtcc gccttcatcc agcgacatct ggcttgggaa catgccctct tccataccga
     1921 cgataaacac ctgcgggaac tccagccctt tcgccgagtg tagcgtcatc aactgcaccg
     1981 catcctgcca agtatccgcc tgcccttcac ctgcttccag tgccgcatgg gagaggaacg
     2041 cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc gttgccgtca
     2101 ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc tgctcgtaca
     2161 tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc ggcatatcgg
     2221 cagtttcttg cgctaaggca tcgattaatt ccataaatcg ctgcaaggcg ctggcagctc
     2281 gcccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt gttaactggc
     2341 gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc cgcgttggcg
     2401 tattcaccac gcgctcaaac gccgcgtcgt cgttacggtt ggcaatcaga cgcagatacg
     2461 agagcgcgtc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa atacggtacg
     2521 gcatactggc ctgcaacagt gcttcttcca gtacacgcga ctgggcgtta ctgcggtaga
     2581 gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca agttttgatg cggttaacca
     2641 caaaacgcgc ttcgtcgagt tcgttaaaag cgcaatagag ggaaataggc tcaccgtccg
     2701 cgccatcggt ccacagtttt ttacccagac gcccgttgtt gttttcaatc agggcgttcg
     2761 cggcgctcag aatattgctg gtagagcggt agttctgctc cagacggata gtttcagcac
     2821 cggggaaatc attgaggaaa cgctgaatat tctctacctg cgccccgcgc cagccgtaga
     2881 ttgactggtc gtcatcgcca acgatcatca ctttgccggt atcgcccgcc agcaggcgga
     2941 tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggatg ttggtaaaac
     3001 gttcgcggta gtgttgcagg atatgcggct tgttaagcca caactcgtga gcgcgcagca
     3061 gcagctcggc gaagtccacc aggcccgcac gatcgcacgc ttcctgatac gcctgataca
     3121 ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc ggacgcaggc
     3181 cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac tgcttctcgt
     3241 cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttca ctgtcgagga
     3301 tctggaaatc ctgcggtaga ttggcgtcca tatggtgcgc acgcagcaaa cggtgcgcca
     3361 gcccgtggaa ggtgccgacc cacatgccgc cctggctggt gcccatcagt tgcccgatac
     3421 gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc gagtatggcg
     3481 agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc gtcttaccac
     3541 tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct tcgcgctgtt
     3601 tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg taaaaatagg
     3661 ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca gatttactga
     3721 ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgcggca gtaaacggct
     3781 gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct gcattccgct
     3841 gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga tctcgccgat
     3901 cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt ttgagcgccc
     3961 atgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca gttccggctg
     4021 ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt ttaaggtgtc
     4081 gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa tcattgctgc
     4141 gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg cttgttcaat
     4201 cgaacgaaaa cgccaacgcg tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc
     4261 ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat gataattttg
     4321 cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat cgtaaagggt
     4381 atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa aacgcattat
     4441 ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg ttgaaaatca
     4501 agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagcagctc ctgcacacca
     4561 cgaagatcgc cgctcgactc cagcatatgc gtggcgaacg agtgacgtaa tttatgcgga
     4621 tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt ctgcacatta
     4681 cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc gctaccaaac
     4741 aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc aatcggcagg
     4801 cggcgctctt tgctgccttt ccccattacc cacacttcac cagactccag gtcgaggtgt
     4861 ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat cacttccagc
     4921 attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tgtccagcag ccgattcata
     4981 tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga aacacctttc
     5041 gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct acgtagcgca
     5101 gaaagccgta acgcgaggct tgctgctccc agccctttac ggcgactgcg tacagcaaaa
     5161 ttgcgcacca tcgtcacatc acattgctgc cagctttgca ggccgttttc gctggcaaaa
     5221 ttgatgatcg cctcaagctg acgctggtag ttaagcaggg ttatcgggct aagctggcgc
     5281 tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt catacgcgtt
     5341 caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt aactgcgttc
     5401 cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacgaca cccaaatcag
     5461 catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc ggtagcacca
     5521 ccagcagctc tggtccgtta agcggcccaa gatagtgctg ttcctgcccc aaacgctgaa
     5581 tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc gacggcgcac
     5641 ctaagcgcca gcgatccgga aacaggcgca gactcgcacc tgccaggccg agatcgcgcg
     5701 cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg agactgcgct
     5761 gcaggtagag taggcgataa aacaggcctt cgttggcgat agcctgttcc atcaacagcg
     5821 ccatgttctc ttccagaaca tgaatatgat tacgtgcgcg ggccatgtgc cactcgacca
     5881 acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg cgcgcattac
     5941 ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca tcaagctccg
     6001 tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc cgtcgtagac
     6061 atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg cgatatcaag
     6121 acgaccgccg gggagttcca cgcgtacttc ttcggccagc aaaccttgct gaatccctac
     6181 tgcaaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc cacgctcata
     6241 aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat tggcgcgctc
     6301 cggaaaacgc tcgtggcttt ccagaacagg accaagcgtt tctaccgccg cggtatcgac
     6361 atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc ataagattgt
     6421 ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa acggcacggc
     6481 ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat cggtgacggt
     6541 cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg tcagtccttt
     6601 cagacgcaca aaacgggcaa agcagcgcgc accgttgccg cactgcgcca cttcactgcc
     6661 atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg gctcaaccac
     6721 cagcagttgg tcaaacccta cccccaggtg ccgatcagcc aggcgacgaa tcagctccgg
     6781 tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc caaggccatg
     6841 cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg acttttagta
     6901 attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca ccgtggattg
     6961 cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag gcgggaaata
     7021 gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata cagtgagtgc
     7081 cttaaacacg tttttcatta tggattgcct gtaaagttca t
//
LOCUS       DAJVQG010000178         7106 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJVQG010000178 DAJVQG010000000
VERSION     DAJVQG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7106
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.178"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..2852)
                     /gene="hrpA"
                     /locus_tag="OQM83_003561"
     CDS             complement(<1..2852)
                     /gene="hrpA"
                     /locus_tag="OQM83_003561"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCS1521732.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMT"
     gene            3053..3658
                     /gene="azoR"
                     /locus_tag="OQM83_003562"
     CDS             3053..3658
                     /gene="azoR"
                     /locus_tag="OQM83_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCS1521733.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(3709..5025)
                     /locus_tag="OQM83_003563"
     CDS             complement(3709..5025)
                     /locus_tag="OQM83_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HCS1521734.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(5015..6772)
                     /locus_tag="OQM83_003564"
     CDS             complement(5015..6772)
                     /locus_tag="OQM83_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCS1521735.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(6788..>7106)
                     /locus_tag="OQM83_003565"
     CDS             complement(6788..>7106)
                     /locus_tag="OQM83_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HCS1521736.1"
                     /translation="RKVVPKVSPGKTLEGLVGGVITTMIASMIIGPLLTPLNTLQAIL
                     AGLLIGISGFCGDVVMSAIKRDVGVKDSGKLLPGHGGILDRIDSLIFTAPVFFYFIRY
                     CCY"
BASE COUNT         1784 a         1946 c         1712 g         1664 t
ORIGIN      
        1 gtcatccgcc gtaactcgcg ctcaaggctg tcgagcaacg gtaactccag cggtgtgacg
       61 cggcctaaaa acgcttcggc gtagtttggt gcaggcacaa aattacggcg taccggtttc
      121 ggcaacgatt taatcagagc aatcaccaat tcgcggcgca gaccggggat ctgccattca
      181 aacccgcttt cctcaacctg gttaagtaac ggcagcggaa tatgtacggt cacaccgtcc
      241 gcgtccgcgc ctggctcaaa ctgatagctc aaacgcagct tgagattgcc ctgatgccag
      301 aagttcgggt aatccagctt tctgattttt tccgcccctt ctttgatcaa catgctcttt
      361 tcaaagttga gcaaatcagg cgtttcgcgg ctgacttttt tccaccagct atcgaagtga
      421 cgagcggaga ttacatcgtg gctgatgcgc tggtcgtaga actcaaacag cgtttcgtca
      481 tcaaccagaa tatcgcggcg acgtgatttg tgttccagct cttccacttc ggcccgcagt
      541 ttcaggtttt cacggaagaa tgcgtgacgc gtctgccagt caccttccac cagagcgtgg
      601 cgaataaaga gttcacgaca taacgccgaa tcgatctggc tgtagttgac cttgcgcgca
      661 gcaacaatcg gcaaaccata aacagtgact ttttccgttg ccatcaccgc gccctgcgcc
      721 cgttcccagt gcggttcgct gtaggtgcgt ttaatcaaat gctgagcaac gggttctacc
      781 cattccgggt cgatacgcgc agcaatgcgc ccccacaggc ggctggtttc taccagttcc
      841 gccaccatta cccatttcgg cggctttttg aataaaccag aaccggggaa gatggagaaa
      901 cgcgcgttac gtgcaccggt atattcctgt ttatcggcat ctttcatgcc gatatgggaa
      961 agcaaaccgg tcagtaaggc gatgtgaatt tcacgatact ccgccggttc gctgttgacc
     1021 ggaatgccaa gttctttcac cacctggcgc aactgggtgt agatatcctg ccattcgcgc
     1081 acgcgcagat agttgaggta atcggtacga cacaggcgac ggaaggcgtt ggaagaaagc
     1141 gccttttgct gctcgccaag ataattccac agatttacaa acgcgaggaa gtcggattct
     1201 ttgtcgtgga agcgacgatg tttttcgtcc gatgcctgct gtttgtccat cggacgttcg
     1261 cgcggatcct gaatggagag cgcggacgtg ataatcatcg cctcacgcac gcagccatgt
     1321 ttttgcgctt ccagcaccat acgcgccaga cgtgggtcga caggcaactg cgagagctgg
     1381 cgaccgagcg gcgtcagttt ataggcgctg gcctgttcat ccgtggtgat cgcgcccagt
     1441 tcttcgagca gacgcacgcc atcctggata ttgcgtttat ccggtgcttc gacaaacggg
     1501 aacgcagcga tatcgcccag ccccagcgcg gtcatctgca aaataaccga ggccaggttg
     1561 gtacgcagaa tctccggatc ggtaaactcc gggcgcgaga ggaaatcgtc ttcggaatag
     1621 agacgaatac agatcccttc ggacacacga ccacagcggc ctttacgctg attggcagac
     1681 gcctgcgaaa tgggttcaat cggcaaacgc tgcactttgg tgcgatagct gtagcggctg
     1741 atacgcgctg taccggggtc gatgacgtat ttaatccccg gtacggtcag cgaggtttcc
     1801 gcgacgttgg tcgccagcac aatgcgccgt ccgctgtgcg actggaacac gcggttctgt
     1861 tcgctgttcg aaagccgcgc ataaagcggc aagatttcgg tatggcgtaa gttcagcttg
     1921 ttcagcgcat cggcggtatc gcgaatttcc cgctcaccac tcataaagat cagaatgtcg
     1981 ccagggcttt cctgactcag ttcgtctacg gcgtcaaaaa tcgcctgcaa ctgatcgcgc
     2041 tcggtgtcat cggcttcttc aacaatcggt cgatagcgca cttccaccgg ataggtccga
     2101 ccggagactt cgataatcgg cgcattatta aagtggcgcg aaaagcgttc cgggtcgata
     2161 gtcgcggaag tgataatgat ttttaggtca ggacgccgcg gcagcaactc tttcaaatag
     2221 ccgagcagaa aatcgatatt caggctgcgt tcgtgcgctt cgtcaataat gatggtgtcg
     2281 tactgcatca gcaggcggtc ttgctggatc tccgccagca ggataccgtc ggtcatcagc
     2341 ttgaccatcg tgttatcact tacgtgatcg ctgaaacgca ctttgtaacc gatgcaaccg
     2401 cccggctccg ttttcagctc ttccgcaata cggttcgcca ccgttcttgc cgccaggcga
     2461 cgcggctggg tatggccgat cagcccttta atcccgcgcc ccagctccat acagattttc
     2521 ggtaactgag tcgttttacc agaacccgtt tccccggcga cgatcaccac ctggtgatca
     2581 cgaatcgctt cgagaatgtc ctgttttttc tgactaaccg gtaaattgtc aggataagta
     2641 atttccggtc gtgccgcttc acgcagcagg actttccctg ccgcctggtc aatctctttc
     2701 gccatctcct ggaaaatggc ctgttgtgca tcaggatttt taaccttctt cacgccgtgc
     2761 agacggcgag aaaaacgcag tctgtcacgc agcatcagcg aatccagccg ctgctgcaag
     2821 gccgtaaagg tcaatttttg ttgttctgtc ataacgttag tgggcagtag tctgcctgat
     2881 taattttctt tgaaatgata gatatagagt accacatcac ggctttctat gcgttgttca
     2941 ataaattcga acataggctt cgatttattg cgctatctct gtggcaggat tgtgaataaa
     3001 gtgtcaacaa gcaacggggc atcgcccatt caaacatcta taaggaaaca ccatgagcaa
     3061 ggtattagtt cttaaatcca gcatcctggc agggtactct cagtctaatc agttgtccga
     3121 ttattttgtt gaacaatggc gcgaaaagca ctccgctgat gaaatcaccg ttcgcgacct
     3181 ggctgcaaat ccgattccgg tactggatgg cgaactggtt ggtgcactgc gtccgagcga
     3241 tgcgccgctg actccgcgtc agcaggaagc tctggcgctt tccgatgagc tgattgccga
     3301 gctgaaagcc cacgacgtta tcgttattgc ggcaccgatg tataacttca acatctcaac
     3361 tcagttgaaa aactattttg acctggttgc acgcgcaggc gttactttcc gctataccga
     3421 gaacggtccg gaaggtctgg taacgggtaa aaaagccatc gttattacca gccgcggtgg
     3481 gatccacaaa gatggaccaa cggacctggt gacgccgtat ctgtccacgt tcctcggctt
     3541 tatcggcatt actgatgtga aatttgtctt cgccgaaggg atcgcatacg gtccggaaat
     3601 ggcagcgaaa gcgcagtctg acgcgaaagc agccatcgac agcattgttg ctgcataacc
     3661 ggacacgatc ttgatgtgga ctgtagtcca gtccacatcc caatccactt acctgttttc
     3721 ccataatttc agcatcgctt tgtgatcttc cttaagcaca atatgcgagc gtctggctcg
     3781 aataaagcta atcgcttcat caacggtttt acagtatccg taacataaca gccatgccgc
     3841 cactaccagc gcactgcgcg ataatcccag tgcgcaatgg accagaacgc tgccttgctc
     3901 ttcgcgtaat gtttccagca tcgccacggc ctgtcggagc tccccctctt ccggaaccac
     3961 cagatctagc atcggtacac agaaatagag tcgatctttt gtcgctcgtc cacgagggaa
     4021 ttcaaaggtg acgtccagaa ccgcattctg tgccggaata tgtcgtggaa acgcccctaa
     4081 ataaacacca gcagtcattt tgctcaccgg ctccaggcga cgacaaaacc aacgcataga
     4141 tagccacatc ccgatgcgcc agggcaatgt cagccagtaa acggcgggcg ggagtttccc
     4201 ctgactatct ttccctgttg ttatcgcgcc aagcccaccg tacccacggc caataatgag
     4261 tagcgataat actggccaac ataaccagac tgaccaccat aactgaacca tcatcattag
     4321 ctccatcaac acaatgcacg cgcacgcgcc tacgacatat ggcagtgcta ttttgattcg
     4381 acgttgatca ggtttctgat aattccaacg acggtcgacg ggcaccatcc agtcaatcaa
     4441 catacctacc gccagccctg tgatgacatc aataaaatga tgctgccatg tcgtcagcgt
     4501 cgaaatggcg atgagtaaaa accatccgcc gcagacttta cgccacctca cagccagatg
     4561 ctgacgaaag tgacgccaaa gtagccagca gagaataata tgcagcgaag gagactggtt
     4621 ataaggcaga tcaaacagtt caagttgcga aaatagccat cccgtcaccc cactcacttc
     4681 aggacggata aaactaaact tcagcggata gagcaaaaaa ccgcagcagg ccattaccgt
     4741 tgccagaata agccggtgga caagtcggcg ctgttcgaat gtcgtgctac aaacgaacag
     4801 cgaaaatcca tataaaagat ccagactcca gtaaggaaca atagtccagg gaagaaaagg
     4861 gatcgccgtt tcccaaccga atacctgact gggaatatca tggctattac tgtcctgaac
     4921 cgcggtgaac tgattaagag atccataggt gaagaagaaa aacggggcca gcaacaataa
     4981 ccagccagct ccttgtagca atacgttccg gcgttcagtt atcacgacgc accgccatcg
     5041 aaaccgtaaa aatgccccac tcatcaatcc gttgtgtgca tttatcaaat ccagcatcat
     5101 gcacgagcaa atccatctcc ccttgcgtac gcacgcgcat tacccacagt ttaccatctt
     5161 tatgactggt taacaccccg gcaatcagct ccagttgtgg gtgccacggc tgcccggtgt
     5221 agatgagaat gccacccggt tcgatggcat tggcaagacc tgcgagtgag ttttttacct
     5281 gctcattttc gggaaacagc tcatacagtc cagagacaat cgccagcgta ggccgcggag
     5341 ttaacgcgct gagttcctcc gggttaaacg catcgccctg ttcaaaacgc acccgcccag
     5401 acattccccg ttgggcaatc atctcttgcc cctgtgcaac atttaactca ctgtaatcac
     5461 gtaataaaat atcgcttacg gcaggctcgt ttgccagtgc atccagtaca tagcgcccat
     5521 gccctgcggc aatgtcaacc acgcggacgg ctaaaccttt gacgtggaga tcggcaacgg
     5581 cttgtttaat cagtatttgt aaatgagttt tgcgctggcg aataccgcgc cagccaacac
     5641 tgttcaggta aattttgtcg attaagcgcc cgaatgcgtt actgccttgc ggttgatttt
     5701 gatagacata atccagcgaa ctgcctgaat caaagccagt gctcattccg agatgcaggc
     5761 ccgcagagtg cgtcccgaac aatttcatcg ctttacgcat aaagcgatac gccaaatcaa
     5821 ccggcgataa tggcacgggt ccacctgaaa ggaggcgcca gcgatccgct gatggtccag
     5881 tgcggtcttc atgctgataa tcaaattttt gtgacttgtt agcatataaa cgacgaataa
     5941 agctttgcat tttttcaaat gcctgcgccc tgttctcttc acccaacgtg tcgtgataaa
     6001 agcctggcag cagatgcagc tctttcagag ggctacgtaa tctctgataa aaatcaatct
     6061 gcggttggcg atgcaccaca tagtcatcgc ctgatatcag aagttgcgtg gggagcgtaa
     6121 tcgccgccgc atctctaata atacgttcag acgttttata gagatcgagc aagatattga
     6181 cggcaatcgc ccgtgtgatc agcggatcat tattgaaact cgccacccgt tgccgatcgt
     6241 gggtcaaata gcgtcttttc acataggaat taataaaaaa cagaccacgc agacgatgcc
     6301 atagcgtcag cgcaggacgt gccagcggca catacaattt aaccttaaag gccggagaag
     6361 ccagcaccag cccgcgaatt gcaggcgcat aatcatgaac ccatgtggca accagcactg
     6421 cgccgacgct ttgcgcgatc acaaccacct cttccagtcc aacctggctg tcgctggcag
     6481 caaaacggac aaattcatcg acatcctgca ctgaacgcgc aagagatggg ctataaccac
     6541 gcggccccga agtttttcca tgcccccggg catcccatgc ataaaaagca gtatctggca
     6601 tcgccaattc atcaacgata tgttgtagac gaccagaatg ttcatggcca cgatgaaata
     6661 agacgatgac ctttttcgcc cctggctgta aagcgggcca gtgccgataa aacaacgctg
     6721 tattatcact ggtggtaaaa aaatgttccc cagagatgcg tgaattttcc attttctcta
     6781 ttttccttca gtaacagcag taacgaataa aataaaaaaa taccggagcg gtgaaaatta
     6841 atgagtcaat cctgtcaagg attccgccat gtccaggtaa tagtttcccg ctatctttaa
     6901 caccaacatc tcgtttgatg gctgacatca ctacatcgcc gcaaaaacca ctaataccaa
     6961 ttaacaaacc cgctaatatt gcctgtaatg tatttagcgg tgtcagtaac ggtccaatga
     7021 tcatcgacgc tatcatggtg gtgattacgc cgccgaccag accttccagc gtttttcccg
     7081 ggctgacttt ggggaccact tttctt
//
LOCUS       DAJVQG010000179         7063 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJVQG010000179 DAJVQG010000000
VERSION     DAJVQG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7063
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.179"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..302
                     /locus_tag="OQM83_003566"
     CDS             <1..302
                     /locus_tag="OQM83_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCS1521737.1"
                     /translation="QRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVP
                     SSAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(391..903)
                     /locus_tag="OQM83_003567"
     CDS             complement(391..903)
                     /locus_tag="OQM83_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCS1521738.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1098..1862
                     /locus_tag="OQM83_003568"
     CDS             1098..1862
                     /locus_tag="OQM83_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521739.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1813..3216
                     /locus_tag="OQM83_003569"
     CDS             1813..3216
                     /locus_tag="OQM83_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCS1521740.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            3216..4682
                     /locus_tag="OQM83_003570"
                     /pseudo
     CDS             3216..4682
                     /locus_tag="OQM83_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            4573..5316
                     /locus_tag="OQM83_003571"
     CDS             4573..5316
                     /locus_tag="OQM83_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCS1521741.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5320..6540
                     /locus_tag="OQM83_003572"
     CDS             5320..6540
                     /locus_tag="OQM83_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCS1521742.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            6544..7050
                     /locus_tag="OQM83_003573"
     CDS             6544..7050
                     /locus_tag="OQM83_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521743.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         1873 a         1684 c         1934 g         1572 t
ORIGIN      
        1 gccagcgtga tgctgatgca ctcgatgcta aatacacgaa ggagttagct gatgcgaaag
       61 ctgaaaatga tgctcttcgg cgcaagcttg ataatggtgg tcgggtgctc gtcaaaggaa
      121 aatgccctgt gccatcctca gccgaaacct ccagcgcctc cggcatgggc aatgatgcca
      181 ccgtcgaact ctctccagtt gctggacgaa acgttctcgg tatccgggac ggaatcatca
      241 gcgaccagac agcattgaga atgcttcagg agtacatcag gatccaatgc cttggggggt
      301 agcggtaatt ttactcatta tccttcaaat cagattctgt tgtcagagga atgggggaag
      361 ctggattcgc aagagctttt tatgggcagt ttagctaaaa aaaggcaaca cgaagctaaa
      421 actaccaaca taaagtgctg accagctagc agctgaaatc agactcactg tatacacttt
      481 cactggatga agttgcaaca ttcctgccac caaaggagca atataacgca gcacggcgat
      541 aaagcgcgaa gtgaaaagta ttgacacaga gttattttgc aactgaaggc gaacccgttc
      601 tattgtattg gaacggcttg atattataca tgcaagttga gggatatgcc caattattat
      661 accaagatgg taattaacga tagtaccgca ccatgcgcca ctcatgaccg tgattccagc
      721 ttcccatggt gaaagagtcg tcttgctgac tgtaattaca gtggccatca taattgatgc
      781 tggtggcaat atcgccgaaa tgagtaaagt ggattttgtg aacgctatta taaaaagaag
      841 cccccatagg cgatatggat gaagtacaaa atagttcatc aaagcattta tccactccat
      901 caatgtcttt ccttctgtga atttttcttt gctatttaac cgtgatacga cttaatttag
      961 gcttaaacac cttacattta caaggtactc ctgtgggggg ccctgccacg gagcgtcgca
     1021 ggagtgagtg atgaggaaga attaaagcaa cctattgtga caggcagcga taacagtaaa
     1081 tcacaggaag cataatcatg gcaaaaccgg actgggaggc catcgaatcg gcataccggg
     1141 ccggagtcct tagcctccgt gatataggcg agaaatacgg cgttacagaa ggggctatca
     1201 ggaagagggc caaaaagctt ggttgggcac gcagtggcgg tacgcaggtt tgcaaaaatg
     1261 gtacgcaaaa aaggaaagtg cgtaccagca gaaagcctgc cattactggc cttacacaaa
     1321 aaagtacgca actaaaaaca gaatctacac cggatacgaa accgatacgc ggaatgcgta
     1381 ccgatccccc gactaaccca ttccaacccg gtaaccagca ggcattaaaa cacggtggtt
     1441 atgcccgtcg ccttctgctc aaagatgagg tgatagagga cgctaaagcg ttgacgctcg
     1501 aggacgaatt atttcgcctt cgtgctaaca accttgtcgc tgcagagaat attggtcggt
     1561 ggttggtgtc gctggaagat gctaatgggg accaggaaag gaagatgctg attgaaaata
     1621 tcagcgccgc cgagaaagca atgatgcgca atacagttcg tattgagtcc atcgttggca
     1681 cgcttgcgac ggtaggaaaa atatttgctg atacagccta ccgcaaggcc gccactgata
     1741 aggtgtctct ggaggctgat cgtcttcgcc gtgatgcagg tattgatgat ggcaatggag
     1801 agcgtgacct caatgacttc tactctgaca tccaaaccga cgctgaatcc ggctttacgt
     1861 agtttctgga ctatgcgggc acgtaacaaa gtgctttatg gtggtcggtc atcgtcaaaa
     1921 tcatgggatg ccgctggcat tgccatattt ctgtcgaata aatacaccct gcgtttttgt
     1981 tgtgcccgtc agatccagaa taaaatcgaa gagtcggtgt ataccctgct caaaattcag
     2041 atagacaggt ttggtctgcg gcaccgtttc cgtattctga acaacaaaat cattaaccgg
     2101 gttactggct cggaatttgt tttttatgga ttatggcgca acatcgaaga aattaagtca
     2161 ctggagggga tcgatgtgtt gtggctggaa gaagcccacg cactgacgga ataccagtgg
     2221 aaaattctgg agccaacgat ccgtaaagag ggttcggaat gctggttcat attcaacccc
     2281 ggacttgtta ctgatttcgt ctggcgtaac ttcgttgttg atccgcccga aggcactctc
     2341 atccgcaaaa ttaactatga cgaaaatccg tttctgtctg acaccatgct taaggttatc
     2401 gacgcggcgc gacgccgtga tccggatggt tttaaacatg tgtatgaggg cgttccggag
     2461 tctgatgatg atgcggcaat catcaaactg tcttggatag aagccgcagt ggatgcgcac
     2521 aaaacgttaa atttcgaacc cagtggaaga aagcgtattg gctttgacgt ggctgacagt
     2581 ggtacagata agtgcgctaa cgtttaccgt cacggatccg ttgttttctg ggccgacgaa
     2641 tggaaggcca aagaagatga attactgaag agctgccagc gtacttatca ggcggcgctg
     2701 gagcgtgaag cagatattgt ttacgactct atcggtgttg gtgcgtctgc cggtgctaaa
     2761 ttctctgaaa ttaacgctga ccggaagagc gagaacgcat acgcgcgacg tgtgaattac
     2821 cagaggttta acgccggtgc tggtgtgcat gagccagatg acgagtacaa cggcatcccc
     2881 aacaaagact ttttcgcaaa tcttaaggct caggcatggt ggctggtggc tgaccgtttc
     2941 agaaatacgt ttaacgccat taacaacgga gaacagtatc ctgtggatga gctgatcagc
     3001 atagattctc gttgtccgtt gcttgaaaag ctgaaactgg aactgacaac acctcatcgt
     3061 gatttcgacc gtaacggacg tgtgatggtc gaaagtaaaa aagacctcgc aaaacgcgag
     3121 ataccgtcac caaacgttgc tgatgcattc attatggcct tcgcgccaat cgatacatcg
     3181 ctggatatct gggaacagct ggggagacag gcctgatggc acgaaacaaa caagccctgc
     3241 ggcgaactgt gcaggccaca gctgatggtt atgagaattt tattgcccgc gtagggatgc
     3301 agacacctaa ccagcactca gcatccacct accgggctaa tttcaccagt cgtaaccgca
     3361 tgctggtgga atggtcctat cgttcatcct agatcatcgg cgaagcagtc gatgctatcc
     3421 cggatgatat gacccgcaaa ggcattcgca tcacttcgga aattgatgca aaagatcgtg
     3481 gcattctcga atcacaactg gatgagttgc aaatctggga tgcgctgaat gacgtgctga
     3541 aatggtcgcg cctctacggc ggcgcggtgg gtttcatcat gattgagggg taggcaccaa
     3601 tgaccccgct gcgacccgaa accatcggta agggcaagtt taaggggatt ctcccgctcg
     3661 accgctggat ggtcgacccg gcactgaccc gccgcattaa agatatgggg ccggacctgg
     3721 gtaaacctga gttttacgat gtggtgacca cagcaacggg aattcctgcc tggcgcattc
     3781 atcacagtcg cctgattcgc tttgatggcg tcacgttgcc atttcagcag aagatgaccg
     3841 agaacgaatg gggaatgtcg gttgtagagc gtatctggga tcgtcttacc gcgttcgaca
     3901 gcgctactgt cggcgcggcg cagctggtct acaaggcgca tttgcgtacc tacagcgtgg
     3961 agaagctacg cgagcttatc gcacttggtg gtcctgcgta tgaagcgttg ctgaagaata
     4021 tcgacctgat tcgacagttc cagagtaatg aaggcatgac gctcatggac tcgcgggata
     4081 agtttgaaac ccatcagtac agcttcagtg gtctggatga catcctttcg cagtttgtag
     4141 aacagattag tggcgctgtt ggtatcccac tggtgcggtt gttcggacag tccccgaaag
     4201 gattttctac cggtgatgca gaccttgcca actattacga tcgcatcagt tcgttgcagg
     4261 agaggcgttt acgtcttccg gtgcggcgga tactggacat catgcatcgt tcggaacttg
     4321 gcaagccgct gccggacgat ttcacgtttg agtttaaccc gctctggcaa atgtctgatg
     4381 tcgatcgctc aacggtggcg ttaaacacca ccaacgcaat cagtacggcg ctgggtgatg
     4441 gtctgatgac actgaaagcc gctatgactg atttgcgaga aaattctgac gtaaccggca
     4501 tcggggcatc cattaccgac gaggacatcg agaatgccga agatgaagcg ccgcccggca
     4561 tcggcgaacc tgatgacgaa ccgcaggagc cgtcaggcgg aaatccggta tcgaaccagc
     4621 ctacgcagga tagcgagggc ggtcggagac atcgtaaatg gtcgctacga tggttcaaat
     4681 gacagtatca cggaaattat tgcggcgctg gagcgttaca gtgaaatcat caccccctgg
     4741 gcgacaaagg tcgcggaaaa ctttactgcg gacctaaccc ggcagaacga gaaagtttgg
     4801 cggcaacaca gcaagaacat cagtcgcgag ctccgcaatc ttgtggaaag cgctcctgtg
     4861 ggccaggtga tgcaatccat catcgccgaa caggtcaagt acatcaaatc tctgcctctc
     4921 gaggccgcag acagggtgta cgacatccag aacaaagcga tagaggctgt tgtcaccggt
     4981 ggtcgggcgg agcagtttgc taaagagatt gcatctaccg gcgatgttgc taagtctagg
     5041 gccgatctga ttgcccgaac ggaactggga agagcaacgg gcgcgctgga tatggcccga
     5101 gcgatagcta ttggctcgga tggttatatt tggcgtacag ccgatgatgg cgatgtccga
     5161 gattcccacg atcacatgaa aggtaaattc gtccgctggg attcacctcc aactttggac
     5221 ggcatgaccg gccacgcggg cgagctgcca aactgccgct gctataaaga gatcgtgttt
     5281 gttcgcgttc cattcgcaat gaaaagggca gcataaccca tgaaatactt ttttgagacc
     5341 aggctcgggg aaacccgata ccgcctggct gacggctcgt tgctgtgcaa agacgtgccg
     5401 ataggacgaa caggtaagca gctctatggt gctgatgacc tgccaaaact gaaacccgat
     5461 aagttcggtg aaatagtcgt cacgcgttct cctgagcagg tattccatcc ggccacgctt
     5521 gcctcattcg aagggatgag catcacgatc ctgcatcctg aagatgaaaa cgggaatgtg
     5581 cggctggtaa atcccgagaa ctggaaagag cttgctgtcg ggcacctcca gaatgtccgg
     5641 cgcgggacgg gtgagcagtc tgatttgatg ctggctgacc ttatcgtcaa agacgaaagc
     5701 gccattcagc ttatcgaaga tggcctgcgc gaagtgtcgt gcggctatga cgcggagtac
     5761 gagcagaccg agccaggtaa agccgagcag gtcgatatta ccggaaacca tgtggctctt
     5821 gtccctaaag gcagagccgg aaatcgttgt gcaattggag acagagacac aatggcaaat
     5881 caaaagaaaa actggtggaa ccgcatgcgt gcggccatca agacaggaga tgccgacacc
     5941 atgaacgaac tggtggagtc ggctcccgca tcggttacag gagatgaggg ggatttgccg
     6001 cagggcgtta atctcaacat caacctgtcc ccgcagcaac cactaccgga caaagcacca
     6061 gagatgggcg gagatccaac cggcgacagt gatgatgacc tcaaaacatt actgaaagcc
     6121 ctgctggcta agctggaaag aaatgccacg ggcgataacg ataataagcc tgacgataat
     6181 ccgaccggtg acggcgagga cgatgaagag gaaaccacga ttactggtga ctcagcctgg
     6241 cgtgccgaag ttatcgttcc gggtatcgat ctgagccgta agatgaaacc gaccgcgttc
     6301 aaacgcgagg ttctggcttc tgctgacaaa acgctggttc gccagatagt cggtgatgcg
     6361 gatatccgca aattaccgaa acaatcggtc gacatggcgt ttaatgccgt gtctgaggtt
     6421 gccaaagggc gaaacacccg cgccaccacc ggcgatgcac agcgcctaaa catgggcatg
     6481 accagtatcg cttccctgaa caaacaaaac gctgaattct gggcaaaccg taaagggtaa
     6541 aaaatgaata atgtatttct gtaccggatg cctgttggca ttgccggggc tgtctctcgc
     6601 ccgcaggact taaccgtcga accggtggtc cttaaatccg ataacgcctt cgctgcctat
     6661 ggcctggctg gtaaatacga tgatgacggt tttttcgtgc cgctggcaga tggtgatacc
     6721 gcagacaagg tgaaggggat ctacgtgcgc ccttatccga ccacgtcgca gccggacatg
     6781 gttcgccagg tgggaacagg caagaacttc ccgggcgacg ccatgaagcg tggctacgtg
     6841 accgttaatc tcggttctga ttttgatgcc agcactatca aaaaaggcga tccggtatac
     6901 gttgtcgtct ccactgatga atccatcaaa gtgccgctgg gtggattcat gtccacgtca
     6961 gtcagtggca aaaacgtggt gctgaccaac gctgaattca caggtgccgg tgatgctgac
     7021 ggcaatgcag aaatttcctg gaagatttaa ggaacagacg aat
//
LOCUS       DAJVQG010000180         6890 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJVQG010000180 DAJVQG010000000
VERSION     DAJVQG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6890
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.180"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..1166
                     /locus_tag="OQM83_003574"
     CDS             <1..1166
                     /locus_tag="OQM83_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCS1521744.1"
                     /translation="VQMDGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHP
                     MVAADVQNPHQPKTATGVIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAK
                     AVDVKLAQWIEDNVTFPSTMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVI
                     EDNFVAGRPEWEKAGAELVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMED
                     EHYRYAAYGLMLQEQAPTLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLP
                     QRMLDSVRWHLAHDSKFDLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSS
                     AEGKARVQSLLAIKAIFGDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1202..1405)
                     /gene="ydfZ"
                     /locus_tag="OQM83_003575"
     CDS             complement(1202..1405)
                     /gene="ydfZ"
                     /locus_tag="OQM83_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCS1521745.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1582..2268)
                     /gene="ydfH"
                     /locus_tag="OQM83_003576"
     CDS             complement(1582..2268)
                     /gene="ydfH"
                     /locus_tag="OQM83_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCS1521746.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2357..3103)
                     /gene="ydfG"
                     /locus_tag="OQM83_003577"
     CDS             complement(2357..3103)
                     /gene="ydfG"
                     /locus_tag="OQM83_003577"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCS1521747.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3240..5285
                     /gene="dcp"
                     /locus_tag="OQM83_003578"
     CDS             3240..5285
                     /gene="dcp"
                     /locus_tag="OQM83_003578"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCS1521748.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5330..5848)
                     /locus_tag="OQM83_003579"
     CDS             complement(5330..5848)
                     /locus_tag="OQM83_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCS1521749.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            6124..6516
                     /gene="ydeI"
                     /locus_tag="OQM83_003580"
     CDS             6124..6516
                     /gene="ydeI"
                     /locus_tag="OQM83_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCS1521750.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         1759 a         1604 c         1737 g         1790 t
ORIGIN      
        1 acgtacagat ggatggctta gaaaccgtgt tggctgcgat gtgtgaaccg caaatcgcga
       61 ttgtctctct gacaatcacc gaaaaagggt atttccactc tccggcgacc ggacagttaa
      121 tgctcgatca cccgatggtc gctgccgacg tgcaaaatcc ccaccagccg aaaacagcaa
      181 caggggtgat tgttgaggcg ctggcccgcc gtaaagcggc aggacttccc gcatttaccg
      241 tcatgtcatg tgacaacatg ccagaaaacg gtcatgttat gcgtgacgtt gtcacttcat
      301 acgcgaaagc tgttgatgta aaactggcac aatggatcga agataacgtg actttcccat
      361 caacaatggt ggaccgtatt gtgcccgcag tgacagagga tacgctggcg aaaatcgaac
      421 aacttaccgg tgtgggcgat cctgcgggcg ttgcctgtga acctttccgc cagtgggtaa
      481 tagaagataa ctttgttgcc ggacgtccag aatgggaaaa agcgggagcc gaactggtta
      541 gtgatgtgct gccttatgaa gagatgaagt tgcgcatgct caacggcagt cattcattcc
      601 tggcgtattt gggttatctt gctggatatc agcacattaa tgactgcatg gaagatgaac
      661 attatcgtta tgcggcgtat ggcttgatgt tgcaggaaca agcgccgacg ctgaaagtgc
      721 agggcgttga tttgcaagat tacgctaacc gattaattga acgctatagc aacccggcgt
      781 tacgtcatcg aacctggcag attgcgatgg atggcagcca gaaattgcca cagcggatgt
      841 tggattctgt tcgctggcat ctggcgcatg acagcaagtt cgatctgctg gcactgggcg
      901 tcgcgggttg gatgcgttat gtcggtggtg ttgatgaaca gggaaatccg attgaaatca
      961 gtgacccact gttacctgtt attcagaagg ctgtacaaag tagtgctgaa gggaaagcgc
     1021 gcgtccagtc attgctggcg attaaggcga tctttggtga tgatttgcca gacaatagtt
     1081 tgtttactgc aaaagtgacg gaagcgtact tgtctttatt agcgcatggc gcgaaagcga
     1141 ccgtggcgaa atattccgtg aagtaaaaaa agcaatgccg ccatcggcgg cattcacacg
     1201 cttagttcat gccgagacga atcaggtcca gaggtgccag tttctcttca caaccttcaa
     1261 caactacggt ttttccgcgg cggatttgct cagcggtcag accttcagta tcaatcgaca
     1321 ggatcttgcc agtgtgaccg gtgccgctaa ccataacacg gctgccagtg gtgattgcgt
     1381 tacggttacg atcgtaggtc gtcataggta ttttctcctt tctgatttac agtcgaagcc
     1441 cacgtaacgg gcgcaatata aatactcctg tcgagtgaaa tattttttgt ttttgatcaa
     1501 gctcacatct tttttgatac atgcatgagc cataaggcgg gggaaaagac tctcaccccg
     1561 gatgggatga gaggaaatga attactcttc gctaaaccag tcgctatttt cctggcggat
     1621 ctggcgcacg gattcgctga tttcctgcaa atgttgcgtc attgcacgtt ctaccgcatc
     1681 gccatcacgt ttttgcaggg cagagaaaat atcaagatgc tggcgtaaca gcatttctgg
     1741 tggagaaacg tggtcgaaac tcatatagcg cacgcgatca acggtcgctt tcaggttctc
     1801 aatggtatcc cacgccagtt gacagtcggc aatctgcgtc aggagttgat ggaagttgtc
     1861 atcaagttca aaaaaatcat ccagttgctt gcgctcaatg gcaatgcgtt gctggtgaag
     1921 attttgttcc agttggtagc actggctttc ggtaatcatg ctcgccgccc gacgcgccac
     1981 cgcgcactca atggcctgac ggataaaact gccattgcgc acctgggcca tggaaatttt
     2041 gttgacgtag ctgccacgct gcggacgaat ttgaatcagg ccgttttccg ccagtttaat
     2101 aaaggcttca cgaaccggct ggcgtgacac attgaaacga acagaaactt ctttttccga
     2161 caacggtgtg cctggtgcaa tcaggcaatg gacaatgtcg cgacgaagaa tacgataaat
     2221 ttgctgattg acaggctgtg tgggattaag ttgcgtttcg acggtcattc gttcttgctt
     2281 gtgagtgagt taactgcatg agtctaccac ttttttgtga caagcaataa cccggcagtt
     2341 acgccgggta taaaaattac tgacggtgga cattcagtcc ggcatagctt tgggtaaccg
     2401 gcatcatttc cagggtattg atattgacgt gagcaggcag cgttgacacc caccagacgg
     2461 cttcgctgac atcttctggc gtcaatgcaa cggtattttg ataggttttt tccgctttac
     2521 cgtcatcgcc tttaaagcgg acattggaaa actcggtgcc acccaccaga cccggttcga
     2581 tgtcggtgac gcgcaccgcc gtaccatgca ggtccgtacg cagattcagg ctaaactgac
     2641 gaacaaacgc tttcgtcgca ccgtaaacgt taccaccggc atacggccag ctacctgccg
     2701 ttgagccaat gttaataata tgaccatgat tacgttcaac catacccggt aagacggcgc
     2761 gcgtcatata taccaggcct ttgttgttgg tatcaatcat cgtttcccag tcttcaacgc
     2821 tggctttatg cgcaggctcc atgcccaacg ccaggccggc attatttacc aggatatcaa
     2881 tattgcacca ctcggcagga agcgatgcca gcatctcttc aatagcggcg cggttgcgaa
     2941 cgtccagttg ggcgatatac agattatctc ccagttcgtc ttttaactcc tgcaaccgct
     3001 cctggcgacg gccagtggcg ataactttat gcccttgttg aataaaacga cgagtaatgc
     3061 attcaccaaa acctgccgtt gctccagtta ctaaaacgat catctcactg ttcctcaacg
     3121 cttttgtgtg tcaataacat agcacgtgat gcttaagagc gggtaatact ggtttgcaag
     3181 attgcactcc gttatcagta agcctacgct atggaacatc tcgcgttcag gagaagaaaa
     3241 tgacaacaat gaatcctttc cttgtgcaaa gcacactacc gtatctggct ccccattttg
     3301 atcaaattgc caatcatcac tatcgcccgg cattcgatga gggaatgcag caaaagcggg
     3361 cagaaattgc tgccatcgcg cttaacccgc aaacgcctga tttcaacaat actattctgg
     3421 cactggaaca aagcggagaa ttacttaccc gcgttaccag cgtctttttt gcgatgactg
     3481 cggcgcatac caatgatgaa ttacagcgtc ttgatgaaca gttttccgct gaactggcgg
     3541 aactggctaa tgatatctat ctgaacggtg aattattcgc gcgggtagat gctgtctggc
     3601 agcgccgtga atccctgggg cttgatagtg aatccatccg cctggtggag gtgattcatc
     3661 aacgttttgt ccttgccgga gccaaacttg cgcaagctga taaagcaaaa ttaaaagtac
     3721 tgaatacaga agctgcgacc ctgaccagcc agtttaacca gcgattactg gcagcaaata
     3781 aatccggcgg tctggttgtg aacgatatcg cgcagctggc aggaatgagt gagcaagaga
     3841 ttgcgctggc ggcagaggcg gctcgcgaga aaggtctgga taacaaatgg ctgattccgc
     3901 tgctgaatac cacccaacaa ccggcgcttg ccgaaatgcg cgatcgtgcg acgcgtgaaa
     3961 aactgtttat tgcgggctgg acgcgagcgg aaaaaaatga tggcaatgat acccgcgcta
     4021 tcattcaacg tctggtagag attcgcgcac agcaggcgaa actgcttggt tttcctcatt
     4081 atgccgcatg gaaaatcgcc gatcagatgg caaaaacgcc agaagcagca ctcaacttta
     4141 tgcgggaaat tgttccagcg gcgcgtcaac gtgcgagcga tgaattagcc tccatacagg
     4201 cggttatcga taagcagcag ggcgggttta gcgcgcagcc gtgggactgg gcattttatg
     4261 cggaacaggt acggcgggag aaatttgatc ttgatgaagc gcagctcaag ccatattttg
     4321 aattaaacac ggtgttgaat gaaggtgtat tctggaccgc gaatcagctc ttcggtatta
     4381 agtttgtcga acgttttgat attcctgtct accatcctga cgttcgggtg tgggaaattt
     4441 ttgatcataa tggcgtgggg ctggcgttat tttacggtga tttcttcgcc cgtgattcaa
     4501 aaagcggcgg tgcatggatg ggcaattttg ttgagcaatc aacgcttaat gaaacgcatc
     4561 cggtaattta taacgtctgc aattatcaga aacccgctgc cggtgagcct gcgttgttac
     4621 tctgggatga tgtaataacc ttattccatg aatttggtca tacgctgcac ggcctttttg
     4681 cccgccagcg ttatgccacg ctttccggca ccaacacgcc gcgtgatttt gtcgaatttc
     4741 cgtcgcaaat caacgaacac tgggcaacgc atccgcaggt attcgctcgc tacgcccggc
     4801 attatcagag cggggcagca atgcctgacg aactgcaaca gaaaatgcgt aatgccagcc
     4861 tgttcaacaa agggtatgag atgagcgaac tgcttagcgc cgcacttctc gatatgcgct
     4921 ggcattgcct ggaagaaaac gaagcaatgc aggatgtcga tgattttgaa ttgcgggcgc
     4981 tggtggcgga aaatatggat cttcctgcta taccgccacg ctatcgcagc agttatttcg
     5041 cccatatttt tggtggcgga tatgctgcag gttattacgc ttatctgtgg acgcaaatgt
     5101 tggccgatga tggttaccag tggtttgttg agcagggcgg attaacgcgt gaaaatgggc
     5161 agcgttttcg cgaggcgatc ctttccagag gtaacagcga agatctggaa cgcctgcatc
     5221 gacaatggcg cggtaaggct cctcagatta tgccgatgct gcaacatcgt ggcttgaaca
     5281 tataaacacc accaatggct ggcctgggga tatcgccagg ccagcctggt tatagcagta
     5341 attgcagcag ctgggcgagg gcaaacctca ccgctaaagc taataccgtt tcgcagtcgc
     5401 cagcaaaatg cataaccgca gtgtagttct ggcctttaat atgccacgcg aaccagacgg
     5461 tacccgctgg cgtaccatct tcaccgccct ccggtccgcc gtagccggta atggcaatac
     5521 tgacatcagc atccgcacgc tctatggcac ctgttgccat ttctgctgcc actttctcac
     5581 tcaccgcaga atatcgttca agagactgct ggcttacgct gaggattttc atctttgcct
     5641 gatcggtgaa agtaacaaag cctgcaccgt aaaatttggg tgtatcttca gctgcacaca
     5701 gggcgctagc caacttaccg ccggtgcatg attcggcggt agttaagcgt aattgatccg
     5761 cgataagtct ttgactcaac gcggatgtca ggttttcaat agtgtcggta tctgcgagtt
     5821 gtacaatttt atctttatta atgttcactg ttttttgtcc acgctattat atgtaaaggt
     5881 gatttgcttt ttctgtatgc tcgtccaata taataatttt caaacatata aatccgatta
     5941 ttctgaaaac atgtttaaga ctcttcaatc ttcttaactt tagtgagtca aattctctga
     6001 atatttgaca gctagtgggc tgattttaaa tgccttataa caattggcag tattcttatt
     6061 tggcaattat aaaaatagca accagactta ataagtcaaa ccaaacgcaa ttaaggagtt
     6121 agtatgaaat ttcagtccat tgtattagca agttttcttg tcatgcctta tgcgttggca
     6181 gatgaccagg gcggtttaaa acaggatgca gcgccaccgc cgcctcatgc aatagaagat
     6241 ggctatcgcg gaaccgatga tgcaaaaaaa atgaccgttg atttcgcaaa aaacatgcac
     6301 gatggcgctt cggtttcact acgcggtaat ttgatttctc acaaaggaga ggaccgttac
     6361 gtttttcgcg ataagagcgg tgaaattaat gttgttattc ctgcggccgt ctttgatgga
     6421 cgagaagttc agccggacca gatgatcaac attagcggca gtctggataa gaaaagcgcg
     6481 ccggccgtcg ttcgggtcac tcatttgcag aaataaaaat aaattagcct gatggcctga
     6541 actactgggc cattagggta ttgggttatg ctatttgtca tccttttatt tacgataatg
     6601 cagaggtgat ttacagaatt aaacactttt ttatccgctg attttgtttt aaatcacaat
     6661 gttatctcag agatgactca taacttcgga tttctatgaa aatattgacg taattttgct
     6721 tttctctcgt tagaatagcg cgcacaagga actgtgaaaa aggagtggca atgatcaaga
     6781 agacaacgga aattgatgcc atcttgttaa atctcaataa ggctatcgat gcccactacc
     6841 agtggctggt gagtatgttt cacagcgtgg tcgcgagaga tgccagtaag
//
LOCUS       DAJVQG010000181         6622 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJVQG010000181 DAJVQG010000000
VERSION     DAJVQG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.181"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(24..605)
                     /locus_tag="OQM83_003581"
     CDS             complement(24..605)
                     /locus_tag="OQM83_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521751.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGECFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(652..4509)
                     /gene="sigA"
                     /locus_tag="OQM83_003582"
     CDS             complement(652..4509)
                     /gene="sigA"
                     /locus_tag="OQM83_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCS1521752.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4870..5874)
                     /locus_tag="OQM83_003583"
                     /pseudo
     CDS             complement(4870..5874)
                     /locus_tag="OQM83_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5859..6236
                     /locus_tag="OQM83_003584"
                     /pseudo
     CDS             5859..6236
                     /locus_tag="OQM83_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6275..>6622)
                     /locus_tag="OQM83_003585"
     CDS             complement(6275..>6622)
                     /locus_tag="OQM83_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCS1521753.1"
                     /translation="LVPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGA
                     WLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIA
                     LAAQSSFSQQKQN"
BASE COUNT         1645 a         1471 c         1493 g         2013 t
ORIGIN      
        1 acctcaaaat accgggacag agatcaggcg tgattaatct gcagcatttt taataacaga
       61 agtactgagc ttactttacc tttgatgatg taatagttat ttccgtcggt actcattttt
      121 ccctgaaata atgtcacgga tgtttcttcc tgctgatcct gcaggcgcag tcgtaaggtc
      181 atatttttaa tgagctgaga tccgggtggc aaggggtatt tgctgtcaga tactgggcga
      241 ctgagtctga tgtggcgcgt atacagcccg ttttcgtaca gggtggcctc tgttatggtg
      301 tcagtgacca gcatgtggga gacaatgctg tattcttttt taaagcactc accagaaacg
      361 gtgattgtaa tgataatggt cttctcgatc gagatgtaaa tacccgtttc gttatactgt
      421 ccgtatcgga accatgagaa ctcagttgca ttccagccat gctccagaat atgtgcattt
      481 ttttgcctga tagtttcttt ttttattcgc tcatgtttaa ggcataacag gcagataaat
      541 aatgtcagaa aaagaaagct aataatgaga gcaagagcga tatagtctgt cacagggtaa
      601 tacatattct ctacggtaga agaagggccg caaacgcggc ccgggctgtt atcagaaaga
      661 gtaacggaag ttggcgttga tcgcgttatc cacgttgtat ttaccaaatg ccgatttctc
      721 aaactcaaga ccgaagcgaa gattatcgcg aatttcggcg ttgagaccaa cattcatgag
      781 aatacgaccg tctttttcac ctttgatacg tttctcaccg gacgcatcac gcagtacggt
      841 ttcaccgttg gcaaacaggt caaactggta gccaaggccg gcgcgggctg tgactttcca
      901 gtccttaccg gagaaggatt tacccacatc aacaccggta cgcccaatca gcggattaaa
      961 gtccttatcc ttcatggtca cgctcattcc gcgatcctgc cagtcgaacc gtttacctga
     1021 tacggcccca taaaccagtt ctgcctgcgg ctcaatccag gagtcttctg ttacatgata
     1081 gcggtagcct gcttcagcac ccacatacaa ggaatgagaa ctgtagtctt ttgttccgag
     1141 tccagcaaag gtcgccgagt actcattatc atggtgaaca tacttactaa tcaggtcgat
     1201 ataggcacca gagtcaaata tagcggaagc atacagacct gccccgacgg acttcgtctt
     1261 gccactgaat gcattactgc tggcatgact gtcggtatac gtcatagtca gaccagtgaa
     1321 aaggtcaagt ccatccagct catgtttttt atccacacca atctgcacat gggtgtagtt
     1381 gtcactgtat ccactagaag ctgaacctgc tccgctcatg atgcgggccc atgcaccggc
     1441 ctcgccattg gtgtcacgca gatcccccat tcgtttattc aggttgttga cttctgtcag
     1501 gaaggctttg taattaccgg acatatacga ttttgctgca ccggaagatg ctgtattttc
     1561 agccacctta tatccggtca gggtccacgt ggtatgttcc tgatcctctt tcgtgctgat
     1621 cacaggagat atgttgctga aacctatggc ctgagtactg gctttaaata tctctttatt
     1681 gcttcctttt ggggctgtta tcaggtcaac gtttaatcct gagttattat ttcccacttt
     1741 ctgtatatcg accgacagac tgttattgct tccggttaac tgatttttaa caatgagttt
     1801 atccgattga tttacagact ggctcagatc agcccgcata atcacagcag tattagtggc
     1861 atccagtgtg tttactgtca cagtatggaa acccgaattc tctatgttac taaacaacaa
     1921 aagactgtcg cgggttttta atgattgcag ggctgactgt ccgtttactc tccataaggt
     1981 gttatacatg ctggctgagg cagcaggagc atcaatacct ccggtataag aagtatcaaa
     2041 gccacccagc aaacctgtag ccagcggtgt gtgtgtggtt ttgtccaggt tatcttcttt
     2101 atactggtca acaccaaaca gaatagatgc ttttttgtct gattttatat taccattgac
     2161 ccaggcataa ttttgagcct ttaacgtggc accatcttct gtcatattat atccatctga
     2221 catatagatt gccgggctgt atttattatt ttctgtaggg gtgcctgtta atgtcagggt
     2281 tccggccagt tcaatagctt tatctacata catttttccc tgggcggtaa cagtagctcc
     2341 tttgttgata atcaggtctg tattaattaa ggataatgca ccagtcatag taacagttga
     2401 tttatcacca acagttattt tgctgttttg agcactcaga ctgccgttga aactactttc
     2461 gcctgctgaa tccccggagc gaagctgttg tctgaatcca aaaccttcgc cagtaatgtt
     2521 tcttcctgag aacatgtcaa tgtatgcagt tttatttccc agaacaattt gagagttttt
     2581 agcctctatg tgtcctttta cattagcatc ccggcctata ctcagggttg cgttttctaa
     2641 atttaatttc cggaaattaa atttacgact ttcccagtcg ggctgggaaa aagaggctat
     2701 ctgattgttg gacttatatt ccgtattgta acgtttattt acagtgcttt cctggtcctt
     2761 tatggcagca gaatagtctt tttgacataa aaaaggaatt ggacagttgt tattgccttc
     2821 tttaaatatg gcatgttctg tcgcatgtcc ttgtaatgtt aactgtgcat tccggacaga
     2881 gatatcgttt ttgatatcga ctgagccatc aaagatcaga ttggcattat tgttatgttg
     2941 ctgggtagta ttgataatat gatttatgct tatattacct gatacattcc catggtacat
     3001 atatgcatca gtattagtaa gtgataatgt tgattgcttc acgtttgagt tcgttacggt
     3061 tgttcccgca tctgttgctg cgattttctg aaatgactgg tcatagccgt ttagatccag
     3121 tgttccgcca tttctggtaa aatagatttc gccattaccg ctttcgctta aagcgtcttt
     3181 ggcatttatt tttaccgtac ctttacctcc ggccatgtaa attttattga aggctttttc
     3241 agcactaagg atgacagtcc cattacctat tttaaggtta tttccctgtg ctatttttac
     3301 atccagagta ccactaccta ttttatgcag attatctccg gcaacgccct taacattcca
     3361 ttcaacggtg gtattattat caatatctat cccggcacct ttataagact tatcttttcc
     3421 gtacacttta tatgtctggt tcttatcgaa tataagcccg ccaatgccaa gatcggtatc
     3481 ctgcttgagc gttaattccc ctcctcctga gacagacagg tccttatctt ttataatcga
     3541 tgataactct tgttttttac catccagctg catagtgttt gtgaaattct tttttaaatt
     3601 gtttaccaga atattattaa ataacgagta gtttgtccag gctatattgg tactactgag
     3661 aaaaccaact gtactggtaa caccatggaa aacccatttt tgttgttctt tatcatatag
     3721 ataagaacca gaaccactat cgccaaaaat tggtgagttt ttaaaagggg ttctgttggt
     3781 ataaatgtaa ctcttgtttt tccagtccag attaaaaaga cttgcgctta acaagtcggg
     3841 attatagttt tgtccaaaca gatattgttt gccgtttaat atcgttgagg ttgttccggc
     3901 acctgctctg aagccaatta tttgttcctt accgttgtag tttacgccat atcttttcat
     3961 cgcatcttcg ggagaaagtg agaagtcggc actctccgta acccctgttg tttccacgac
     4021 gaatttattc agacgatgaa ccgaaaagtc gccagatgat actctgttac ttgctttata
     4081 tgctgtctga tcccatgact gtgttgttat tgcatgatgc tgtgtaccgt tatgagtcgc
     4141 agtaacactg tacgcacctc caattgatgt tgttgcacct ttgttggaaa cagcagaaaa
     4201 atcaggtaca ggtagttctg gaaaatgaaa tatctttcca tctttcatca ttaattgaac
     4261 atttgttgca cccggtctga actctcctct gttttgagca aggtcaagat aatcgcgggt
     4321 ccatatatta tttatatcca gcattgctgc aaatgctgtt tcgccataac ttccatatat
     4381 tgaggataaa ataataccga ggataacttt ctttcttgat gtaccgacac taactttccg
     4441 ggtcagttca gaaacagcaa ctaatccacc tgtaatatga ctatatttca gtgaataaat
     4501 tttattcata cgtgaactcc gatatttctg agttgtggtt atgggataac atttaattac
     4561 aacggggaac tatcagtcat ggtacgtaac tgataagggt ataatgataa tcactgtcag
     4621 ttatatttgt atgacagatg taacaaaatg aaaaaaaaga taagcacaag tgattgattg
     4681 gtcagatatt gatcacttgg tgtttgtatt ttgaactttg cgatcaaaat ttgatctttg
     4741 ttgtttccgg tcaatccttt acaggttttc tgtattatcc gggggagtta agtctgtcga
     4801 tgtccgacag acttttgatg atggaaacaa catgcagcga taatgtgcat gctgttttgc
     4861 aggtggaact cagaaggtga actggaactg tgcgaccgca ccgtaagtgt tgtccgtgcc
     4921 gatcaggcct gcaacatcaa ggtgaaccac atcaaacggt gagatcccca ccccggccgt
     4981 gaatgcactg ccgtcgttac cggccagatt ctgacggtaa ccggcgcgca gctgtgccca
     5041 tttccaggca ttaaattctg cgccaatggc ggcaaactga cggttgctgt cggaggtgaa
     5101 accgctggcc ggggtcagat ccacatcaaa tgcggtggtg aacatcgcgt tgtgccagga
     5161 gacaccggcc gtcacctgcg ggcggatcct gaaggtttcc gtgataccgt tcacttcttt
     5221 tgtctcaata ctacggggga tcaggttctg tgcgaccagt cccagcgtcc agttgtcatc
     5281 caggtccgta ctggcaccga tgtcggcgtt gatgccattt ttcgtgttgt ggtagcggtc
     5341 gcctttgaag gcgctgctgt cataatttct gaccagcacg ttgtagttga acaggtcgac
     5401 gcgctggaat ttggggggga agcccagaga ccatttctgc ccggccgttt ccagttccgt
     5461 agccagagca atacccacat cagtgatcag tgccgcgcgg gcaaaggcct gtgatgtcag
     5521 gcggcttttg tccacctcct gcccggtgct gtttgccacc tcttccaggt aagtgaggtc
     5581 agcatcagag actttaccgt cgacactgac ggtgccgtgt gatttaacca tcagtgcggc
     5641 agagaggcga tcaccgggaa gggccgccac agcagaaact cccagctgtg cggcggcatg
     5701 tgtgttacgg aagtcccgga ggtgttcttt caggcgtgcg gcagcctcag gaacaccgtg
     5761 gttactttcg atggcgcggt cgaaggcctt ccactcatca ctgatccggt cggcgttgtc
     5821 ggtgatatta tccggattgg agagctgcgc gccgacggac ggaagcagga tgctgggctc
     5881 atgcgcgacg caaaatccac gatgtatata tcagtactaa aagcgcgacg gcggaagaag
     5941 ctctgaaact aatcggtgaa ctgtacgcca tagagcacga tatacgcggg ttaccggtgt
     6001 ctgaacgcct ggcggtcagg caaatgcaga gtagaccgct cctgacttcc ctgtataagc
     6061 tgatgcagga gaaagaacac acgttatcga agaaatgtcg tctgggagat gcgttccggt
     6121 atatcaggaa gcactgggct gcgttgtgca acttctgtga tgatggcctg gctgaggtag
     6181 ataataatgc agcagaaaga gcgcttcgtg cagtctgtct cgggaagaaa actttttcga
     6241 ggatcactca ttccgtgtct gactgcttca gggtttaatt ttgcttctgc tgactgaatg
     6301 atgattgggc agcaagtgca atatcccaca taaatccagt taactcacga gccaccgccg
     6361 taatggctac ttgtgatttt ttcccttttg ctgtcagagt tcggtaacga gaacacaatc
     6421 gttgttgagc tttccatgca atatctttgg aatattgtgt tacagagtcc ggtcggtaat
     6481 atataagcca tgctccaact tttgcaggtg tacgataaga ccaagcggct tcgtaaagca
     6541 gacgtctcag ttcgctgttt ccaacttttg ttattcctct gggacgaata ctgtttccac
     6601 tggaatattc acctggcacc ag
//
LOCUS       DAJVQG010000182         6611 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJVQG010000182 DAJVQG010000000
VERSION     DAJVQG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6611
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.182"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..2576
                     /gene="yfaL"
                     /locus_tag="OQM83_003586"
     CDS             <1..2576
                     /gene="yfaL"
                     /locus_tag="OQM83_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCS1521754.1"
                     /translation="AELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIE
                     GAGQLTIAQNGSYVLSGAQSMALTGDIVVDDGAVLSLEGDAADLAALQDDPQSIVLNG
                     GVLDLSDFSTWQSGTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVV
                     VDASDGQVSLANNNSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEIT
                     ANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTFTKTGAG
                     TLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGDGAAFVTGADQDIQSIDATS
                     SGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTY
                     LADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVM
                     NGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYD
                     YTLVEDNNDWYLRSQEVTPPSPPNPNPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVG
                     GYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSG
                     GLFSGRWGTDGEWMLGAVGSYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQ
                     KQGAWLDNWLQYAWFSNDVSEHEDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQV
                     IYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATE
                     IEEDGSTISDDAVKRRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVK
                     W"
     gene            complement(2704..3426)
                     /locus_tag="OQM83_003587"
     CDS             complement(2704..3426)
                     /locus_tag="OQM83_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCS1521755.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3573..6200
                     /gene="gyrA"
                     /locus_tag="OQM83_003588"
     CDS             3573..6200
                     /gene="gyrA"
                     /locus_tag="OQM83_003588"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCS1521756.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYYTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6408..>6611
                     /locus_tag="OQM83_003589"
     CDS             6408..>6611
                     /locus_tag="OQM83_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 family protein"
                     /protein_id="HCS1521757.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDF"
BASE COUNT         1571 a         1659 c         1900 g         1481 t
ORIGIN      
        1 aggcagagct aaacgttggc tccacccaac aaacctttgt gcactcattg acgggctttc
       61 agaatggcac tttaaatatc gatgctggtg gcaatgttac tgttaatcaa ggcagttttg
      121 ctggcaccat cgaaggtgct ggtcagctca ccattgcgca aaacggcagc tacgtgctgt
      181 cgggggcgca gtcgatggcg ctaaccggcg atatagtcgt tgatgatggt gcggtgcttt
      241 cgctggaagg cgacgcggca gatctcgccg ctctccagga cgatccgcag tcgatcgtgt
      301 taaacggcgg tgtgctcgat ctctctgatt tctccacctg gcagagcggc acatcataca
      361 atgatggcct tgaagtcagt ggcagcagcg gaacggttat cggcagtcag gatgtggtag
      421 atcttgcagg tggcgacaat ttgcatatcg gcggcgacgg gaaagatggc gtctacgtgg
      481 tggtcgatgc gagcgacggg caggtaagtc tggcaaacaa taatagttat ttgggcacaa
      541 cacaaatcgc ctccggtacg ctgatggtga gcgacaactc gcagcttgga gatacccact
      601 ataaccgcca ggttatcttt accgataagc aacaagaaag cgtgatggag attactgcca
      661 atgtcgatac tcgctctacg acgactgagc atgggcgtga tattgaaatg cgcgccgacg
      721 gtgaagtggc agttgatgcg ggggtagaca cgcagtgggg cgcactgatg gctgacagca
      781 gcgggcagca tcaggatgag ggtagcacat ttactaaaac gggggcgggt acgctggagc
      841 tgaccgccag cggtacaacg cagtcggcag tacgtgtcga agaaggcacc ctgaaaggcg
      901 atgttgcgga tatctttcct tatgcttcgt cactgtgggt cggtgatggg gcggcgttcg
      961 ttactggcgc ggatcaggat attcagtcta tcgatgctac ttccagcggc actatcgata
     1021 tcagcgatgg tacggttttg cgtctgaccg ggcaggatac ttccgtcgcc cttaatgcct
     1081 cattgtttaa cggcgatggg acgctggtga atgctaccga tggcgtgacg ttgacaggtg
     1141 agcttaatac caaccttgaa actgacagcc tgacctatct tgccgacgtg acggttaatg
     1201 gtgatctgac caatacgtcc ggtgcggtta gcctgcaaaa tggcgtcgct ggcgatacgc
     1261 tgacggtaaa cggtgattat accggcggcg gtacgctact gctcgatagc gaattaaacg
     1321 gcgatgactc ggtaagcgat caattggtga tgaacggtaa tactgctggc aataccacgg
     1381 tggtggttaa ctctattaca gggattggtg agccgacatc gacaggcatt aaagtggttg
     1441 atttcgcagc tgatcccacg cagtttcaaa acaatgcgca gttcagtctg gcaggcagcg
     1501 gctacgtcaa tatgggagcg tatgactaca cgctggtgga agataacaac gactggtatc
     1561 tgcgatcgca agaagtaacg ccgccatcgc cacctaatcc caacccgact cccgatcctg
     1621 atcccacacc ggatcctgac ccaacccccg acccggcccc tacgcctgct taccagccgg
     1681 tgctgaatgc caaagttggc ggttatctca ataacctgcg ggcggcaaat caggcgttta
     1741 tgatggagcg acgcgatcac gctggtggcg atggtcagac gctgaattta cgtgttatcg
     1801 gcggacgtta tcattacacg gcggcagggc aactggctca gcaggaagac acttctacgg
     1861 tgcagcttag cggcggcttg tttagcgggc gctggggcac ggatggcgag tggatgcttg
     1921 gggcggttgg tagctacagt gataatcagg gcgacagccg ctcgaatatg accggaactc
     1981 gcgccgataa ccagaaccac ggttatgccg ttgggctgac ctcaagctgg tatcagcacg
     2041 gtaatcagaa gcagggggcc tggctggata actggttgca gtacgcgtgg tttagcaatg
     2101 atgtttctga acatgaagat ggcacagatc attaccattc gtcggggatt atcgcctcgc
     2161 tggaagccgg gtatcagtgg ttaccggggc gtggtgtggt gattgaaccg caggcgcagg
     2221 tgatttatca gggcgtgcag caggatgatt ttaccgccgc taaccatgcg cgcgtgtcac
     2281 aatcgcaggg ggatgatatt cagacgcggc tgggtttaca cagcgaatgg cgtaccgcgg
     2341 ttggtgtcac accaacatta gatctgaatt attatcacga tccccatgcg acggaaattg
     2401 aagaagatgg cagcactatc agtgacgatg cggtgaagcg acggggtgaa ataaaagtgg
     2461 gagtcacggg caatatcagt cagcgagtgt ccctgcgcgg cagcgtggcg tggcagaaag
     2521 ggagtgatga ttttgcccag acggcagggt ttttgtcgat gacggtgaaa tggtaatcgc
     2581 tgtctgacca tcacaattgc cggatgcggc gtaaacgcct tatctggcct acggagagca
     2641 tttgtaggcc tgataagacg ctgcaagcgt cgcatcaggc attggcgcgg ccaaacatca
     2701 acctcactta ttctgcgtgt gcagcatata gttcacatcc acgccggggc cgagcttaaa
     2761 ggaattagtg atcgggttgt aatgtagccc ggttatatgt cgctctttta aactggtctg
     2821 atccacccag ccaagcagct ctgccggttt aataaacttc ttcacatcat gcgtaccttt
     2881 gggcaccatg cgcaaaatat attccgcgcc aaccaccgcc atcagccatg acttgccgtt
     2941 gcggttaagc gtcgagaaaa agacatcgcc gcctggtttc accagttgcg cacaggctct
     3001 gaccactgac tgcggatcgg gtacgtgctc cagcatctcc atgcaggtca ccacatcata
     3061 ctgcccggca tgttttgccg cgtgctcttc cacggtttcc tgcacgtaat ccacctgaat
     3121 gccgctttcc agcgcgtgca gctttgccac ctgcaatggc tcaaagccca tatccagacc
     3181 ggtcaccgtc gcgccttcgc gcgccatact ctcggccaga atgccgccgc cacaaccgac
     3241 atcgagcacc tttttgccaa ataaaccgcc agcacgctcg gcaatatagc ccagacgcag
     3301 cgggttaatg cggtgcaacg gtttgaactc accttcaaga tcccaccagc gggaggcgac
     3361 ggcttcaaat ttagcgatct cttcgtggtc tacgttatgg ttttccggcg atttttcggc
     3421 attcattggc acttctactc cgtaattggc aagacaaacg agtatatcag gcattggatg
     3481 tgaataaagc gtataggttt acctcaaact gcgcggctgt gttataattt gcgacctttg
     3541 aatccgggat acagtagagg gatagcggtt agatgagcga ccttgcgaga gaaattacac
     3601 cggtcaacat tgaggaagag ctgaagagct cctatctgga ttatgcgatg tcggtcattg
     3661 ttggccgtgc gctgccggat gtccgagatg gcctgaagcc ggtacaccgt cgcgtacttt
     3721 acgccatgaa cgtactaggc aatgactgga acaaagccta taaaaaatct gcccgtgtcg
     3781 ttggtgacgt aatcggtaaa taccatcccc atggtgactc ggcggtttat tacacgatcg
     3841 tccgtatggc gcagccattc tcgctgcgtt acatgctggt agacggtcag ggtaacttcg
     3901 gttccatcga cggcgactct gcggcggcaa tgcgttatac ggaaatccgt ctggcgaaaa
     3961 ttgcccatga actgatggcc gatctcgaaa aagagacggt cgatttcgtt gataactatg
     4021 acggcacgga aaaaattccg gacgtcatgc caaccaaaat tcctaacctg ctggtgaacg
     4081 gttcttccgg tatcgccgta ggtatggcaa ccaacatccc gccgcacaac ctgacggaag
     4141 tcatcaacgg ttgtctggcg tatatcgatg atgaagacat cagcattgaa gggctgatgg
     4201 aacacatccc ggggccggac ttcccgacgg cggcaatcat taacggtcgt cgcggtattg
     4261 aagaagctta ccgtaccggt cgtggcaagg tgtacatccg cgcccgtgct gaagtggaag
     4321 tcgacgccaa aaccgggcgt gaaaccatta tcgtccacga aattccgtat caggtaaaca
     4381 aagcgcgcct gatcgagaag attgcggaac tggtaaaaga aaaacgcgtg gaaggcatca
     4441 gcgcgctgcg tgacgagtct gacaaagacg gtatgcgcat cgtgattgaa gttaaacgcg
     4501 atgcggtcgg tgaagttgtg ctcaacaacc tctactccca gacccagttg caggtttctt
     4561 tcggtatcaa catggtggca ttgcaccatg gtcagccgaa gatcatgaac ctgaaagata
     4621 tcatcgcggc gtttgttcgt caccgccgtg aagtggtgac ccgtcgtact attttcgaac
     4681 tgcgtaaagc tcgcgatcgt gctcatatcc ttgaagcact agccgtggcg ctggcgaaca
     4741 tcgacccgat catcgaactg atccgtcatg cgccgacgcc tgcagaagcg aaaactgcgc
     4801 tggtggctaa tccgtggcag ctgggcaacg ttgccgcgat gctggaacgt gctggcgacg
     4861 atgctgcgcg tccggaatgg ctggagccag agttcggcgt gcgtgatggt ctgtactacc
     4921 tgaccgaaca gcaagctcag gcgattctgg atctgcgttt gcagaaactg accggccttg
     4981 agcacgaaaa actgctcgac gaatacaaag agctgctgga tcagatcgcg gaactgttgc
     5041 gtattcttgg tagcgccgat cgtctgatgg aagtgatccg tgaagagctg gagctggttc
     5101 gtgaacagtt cggtgacaaa cgtcgtactg aaatcaccgc caacagcgca gacatcaacc
     5161 tggaagatct gatcacccag gaagatgtgg tcgtgacgct ctctcaccag ggctatgtta
     5221 aatatcagcc gctttctgaa tacgaagcgc agcgtcgtgg cgggaaaggt aaatctgccg
     5281 cacgtattaa agaagaagac tttatcgacc gactgctggt ggcaaacacg cacgaccata
     5341 ttctgtgctt ctccagccgt ggtcgcgtct attcgatgaa agtttaccag ttgccggagg
     5401 ccactcgtgg cgcgcgcggt cgtccgatcg tcaacctgct gccgctggag caggacgaac
     5461 gtatcaccgc gatcctgcca gtgaccgagt ttgaagaagg cgtgaaagtc ttcatggcga
     5521 ccgctaacgg tactgtgaag aaaaccgtcc tcaccgaatt caaccgtctg cgtaccgccg
     5581 gtaaagtggc gatcaaactg gttgaaggcg atgagctgat cggcgttgac ctgaccagcg
     5641 gcgaagacga agtaatgctg ttctccgccg aaggtaaagt ggtgcgcttt aaagagtctt
     5701 ctgtccgtgc gatgggctgc aacaccaccg gtgttcgcgg tattcgctta ggtgaaggcg
     5761 ataaagtcgt ctctctgatc gtgcctcgtg gcgatggcgc aatcctcacc gcaacgcaaa
     5821 acggttacgg taaacgtacc gcagtggcgg aatacccaac caagtcgcgt gcgacgaaag
     5881 gggttatctc catcaaggtt accgaacgta acggtttagt ggttggcgcg gtacaggtag
     5941 atgactgcga ccagatcatg atgatcaccg atgccggtac gctggtacgt actcgcgttt
     6001 cagaaatcag catcgtgggc cgtaacaccc agggcgtgat cctcatccgt actgcggaag
     6061 atgaaaacgt agtgggtctg caacgtgttg ctgaaccggt tgacgaggaa gatctcgata
     6121 ccatcgacgg cagtgccgcg gaaggggacg atgaaatcgc tccggaagtg gacgttgacg
     6181 acgagccaga agaagaataa ttttacttct tcatgccaaa agggagctat ctcccttgtt
     6241 tgaattgaaa agtccaggct gcaaagtctg ggcttttgtc gtattagggc acggtaaagt
     6301 ttggctgtgc ccgtaaaaaa tggctggcta tacacaagga atgtggcaat gagtggtgaa
     6361 aaaaagcgaa aggctggcgg ttctatggtc ttgtaggttt tggcgcaata gtactgcttt
     6421 ccgctggtgt ctgggcgttg caatatgctg gcagtgggcc agaaaaaacg ttgtcgccgc
     6481 tggtggtgca caacaatctg caaatcgatc tcaatgagcc ggacctcttt ctcgacagcg
     6541 actctctgag ccagcttccc aaagatctcc tcaccattcc gtttctccac gatgttctga
     6601 gtgaagattt c
//
LOCUS       DAJVQG010000183         6563 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJVQG010000183 DAJVQG010000000
VERSION     DAJVQG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.183"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..611)
                     /gene="nfeF"
                     /locus_tag="OQM83_003590"
     CDS             complement(<1..611)
                     /gene="nfeF"
                     /locus_tag="OQM83_003590"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HCS1521758.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAV"
     gene            899..1522
                     /gene="nfeR"
                     /locus_tag="OQM83_003591"
                     /gene_synonym="yqjI"
     CDS             899..1522
                     /gene="nfeR"
                     /locus_tag="OQM83_003591"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCS1521759.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHSRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(1629..3149)
                     /gene="aer"
                     /locus_tag="OQM83_003592"
     CDS             complement(1629..3149)
                     /gene="aer"
                     /locus_tag="OQM83_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCS1521760.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            3567..4946
                     /gene="ygjG"
                     /locus_tag="OQM83_003593"
     CDS             3567..4946
                     /gene="ygjG"
                     /locus_tag="OQM83_003593"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCS1521761.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMQLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(4988..5320)
                     /locus_tag="OQM83_003594"
     CDS             complement(4988..5320)
                     /locus_tag="OQM83_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCS1521762.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            5539..6522
                     /gene="ebgR"
                     /locus_tag="OQM83_003595"
     CDS             5539..6522
                     /gene="ebgR"
                     /locus_tag="OQM83_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCS1521763.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1602 a         1774 c         1655 g         1532 t
ORIGIN      
        1 tctaccgcct gctcatcatg tgccagccat tcgatattaa aaccatcaag gtgcgcgaga
       61 taatcctgac aggcgttatc ccgcacgcta accagcgcac taacttgcgg tttaacggca
      121 agtttgctca acgtttccaa gcggcggcgc agtgcgggca ttccggattc atcgcagaca
      181 tacagctgat acgcgtaatc ttccggcacc accagcgaac cgcgcggacc tgcaaccgta
      241 agtttatcgc ccggttgcgc ctgcatcgcc cagccgctgg cgaccccacc gtcgtgaata
      301 aagaaatcga tcgccagttc atggcgtagt tcgtcataca gcggcgtata gtcacgcgac
      361 ggtgggcgtg gtccttccgg ccagacgatg ccctcttccg ttaccgttgg cggcacaaag
      421 tgagcgtcag gttgaggaaa gaagagtttg ctgtgatcgt caaagccgcg cgatgtaaaa
      481 ccgtccagcg cctcgccgcc gaggacaatg cgctgaaaac cggcgctgat gcgctcaacg
      541 cgtaacacag tcagttcacg gaagcgcaga tcattgcgaa cgcgctgcgg gtagcggggg
      601 gagttattca ttgttatcgc cttcgtgatg gtaatcagat atatctaaat aattttcgta
      661 aatgataatg attgttaatc atgataaatg caagcgattt gtagaactga tatgtctata
      721 gtctgataag acgaaccgcc tcttctcagg catcattact caacgccgga tgcggcgtga
      781 acgccttatc cggcctacgt gtgagatgag tccacattta aaaatcattt ttattcttgc
      841 aagagtgctc atatcagata taaattagat atatctaatc aagcaaaagg aggctgatat
      901 gagccatcat cacgaagggt gttgtaaaca tgaaggccag ccacgccatg agggctgctg
      961 caaaggtgag aagtcagaac acgagcactg cggacacggt caccagcatg aacacggtca
     1021 atgctgcggt ggtcgccaca gtcgcggcgg cggtcgtcgg caacgtttct ttggtcacgg
     1081 tgaattgcgt ctggtgattc tggatattct ctcgcgcgat gacagccacg gttacgaatt
     1141 gattaaagcg attgagaatc taacccaggg gaattacacc ccaagcccgg gcgtcatcta
     1201 cccaacgctg gattttctgc aggagcagtc gctgattacc atccgcgaag aggaaggtgg
     1261 taagaagcag attgcgctga ccgaacaagg cgcgcagtgg ctggaagaaa accgcgaaca
     1321 ggtggagatg attgaagaac gcatcaaagc gcgttgcgtt ggtgctgcgc tgcgccagaa
     1381 cccgcaaatg aagcgggcgc tggataattt taaagcggtg ctggatttac gcgtcaacca
     1441 gagcgatatc actgatgcac aaataaaaaa gatcattgcg gtgatcgacc acgccgcttt
     1501 tgatattacc caactggatt aatcaccgat tgccggatgc ggcgtaccct acacaccccg
     1561 caggcctgat aagatgcgcc agcatcgcat caggcattgt gctccaaccg ccggatccgg
     1621 cataccgatt aatgcagcac cgtcaccgcg tcttccagtc ggctggcgcg gtgtttcacc
     1681 atcgccgaca cctgcgcact ctcttccacc agctcagcat ttttctgggt gatcaggtta
     1741 agctcatcca ctgcacgggt caggctggaa agcccatcgg cctgttccag cgttgaatgg
     1801 ctaatctggg cgatcaactg ggtgacgttt ttcacctgtg ccacaatatc ttccatcgtc
     1861 cgtccggcgg cgtgtacctg ctgcgaaccg gattgcacct tatcagcact ggcatcaatc
     1921 agcttgcgaa tatcgttggc agcattagcg ctgcggctgg ctaaatgacg cacttcccct
     1981 gccaccactg caaaaccttt gccctgttcg ccggcacgcg ccgcttccac cgccgcattc
     2041 agggccagaa tattggtctg aaacgcaata tcgttaatca gcgaagtaat ggtgccaatg
     2101 cgctgggtac tgtcggcgat atcgtccatt gtcttgatca ccgtggtcat cgcctcccca
     2161 ccctgcactg ccgcattact ggcggtgata gaaagtttat cggcagccga cgccgtggca
     2221 gagttctgtt tcaccgacgc cgccatttgg ttcatggtcg ccaccgtttg ctgaacgtta
     2281 tcaactgtct gctgggtatg ttcgttcagt tcatcggtgc ctttcgccag cgtctcactg
     2341 ccatttctga cactggacac ctggcttgag acatcgttaa ttagccaacg gcacatcagg
     2401 ccaagctgcc ctaccgcgcg taatgtcagc cccagctcat cgctgcgatt caggtgctca
     2461 acactattac gctctccggt cgccaccttc agtgcctgac gggcgacatt ttctatcggg
     2521 cgcacaatct gccattcaaa acaagcgctt gccaacaata ccactaacac acagaggaaa
     2581 tacgtcacca ccggggcagc aacaaaccaa agcatggccg ccagcaagat aaacatcagg
     2641 gtcatcactc cacgcgcccg ccagcgaagc ggtaatgaag gcagtttacc cagccagcct
     2701 ttacgcacca ccaggccttt atgaatacgc ttactggtac gtccggcgtt cagcgctttg
     2761 tacagcggct ccaccgccgc aatctcttca tccgtcgccc gggtacgaat cgacatatag
     2821 ccactgattt ttccctcgcg caccatcggt accgcattgg cccgcaccca ataatggtcg
     2881 ccatttttgc ggcgattttt cacgatgccg ctccagggct cccctttttt cagggtgaac
     2941 cacatatccg caaacgccgc ttttggcata tccgggtgac gcaccatgtt gtgcggctgc
     3001 ccttgtaact cttgcaaggt aaagccgctc acctgcacaa aagtgtcatt agcatgagtg
     3061 atatagcttt gcagatcggt agtggacatc agagtggtat cgtccgccag cggggtattt
     3121 tgctgggtga catacggatg agaagacatg ttcgcgtcct gtgcaggtta tatggttgtt
     3181 aacttcttgt cagagtttat gtcggccccg ctgcggttat ctttaaccga ttaatttgat
     3241 ttagatcgca atttgcgatt aaagcacaaa tctaattctt tgatttaaaa tactttcact
     3301 ctgttactat acgaaaacgt taattatctt gcccaaaaat caggcaatta ttgccctgaa
     3361 aacgtgcatt tgcgcagcaa tcatcaaatc catacccgac aaaaaccgtg caaaataaca
     3421 acaaatgtta acagatagca ttaaatattg cacaaatgat aaccgaattt gtgtttatcc
     3481 cgattttcgc gatcgcagcc ggagtggcgc aatccctgca atacttaaat cggtatcatg
     3541 tgatacgcga gcctccggag catattttga acaggttacc ttcgagcgca tcggctttag
     3601 cgtgcagcgc ccacgccctg aatctcattg agaagcgaac gctggatcat gaggagatga
     3661 aagcacttaa ccgagaggtg attgaatact tcaaagagca tgtcaatccg gggtttttag
     3721 agtatcgcaa atctgttacc gccggcgggg attacggagc cgtagagtgg caagcgggaa
     3781 gtttaaatac gcttgtcgac acccagggac aggagtttat cgactgcctg ggaggttttg
     3841 gaattttcaa cgtggggcac cgtaatccag ttgtggtttc cgccgtacag aatcaacttg
     3901 cgaaacaacc gctgcacagc caggagctgc tcgatccgtt acgggcgatg ttggcgaaaa
     3961 cccttgctgc gctaacgccc ggtaaactga aatacagctt cttctgtaat agcggcaccg
     4021 agtccgttga agcagcgctg aagctggcga aagcttacca gtcaccgcgc ggcaagttta
     4081 cttttattgc caccagcggc gcgttccacg gtaaatcact tggcgcgctg tcggccacgg
     4141 cgaaatcgac cttccgcaaa ccgtttatgc agttactgcc gggcttccgt catgtgccgt
     4201 ttggcaatat cgaagccatg cgcacggctc ttaacgagtg caaaaaaacc ggtgatgatg
     4261 tggctgcggt gatcctcgaa ccgattcagg gtgaaggtgg cgtaattctg ccgccgccgg
     4321 gctatctcac cgccgtacgt aagctatgcg atgagttcgg cgcactgatg atcctcgatg
     4381 aagtacaaac gggcatgggg cgcacgggca agatgttcgc ctgcgagcat gagaacgtac
     4441 agccggatat cctctgcctt gccaaagcgc tcggcggcgg cgtgatgccg attggcgcga
     4501 ccatcgccac tgaagaggtg ttttcagttc tgttcgacaa cccattcctg cataccacca
     4561 cctttggcgg caacccgctg gcctgtgcgg cggcgctggc gaccatcaat gtgttgctgg
     4621 agcagaactt accggctcag gctgatcaaa aaggcgatat gttgctggac ggtttccgtc
     4681 aactggcgcg ggaatatccc gatctggtac aggaagcgcg tggtaaaggg atgttgatgg
     4741 cgattgagtt tgttgataac gaaatcggct ataactttgc cagcgagatg ttccgccagc
     4801 gcgtactggt ggccggaacg ctcaataacg ccaaaacgat ccgcattgaa ccgccactga
     4861 cactgaccat tgaacagtgt gaactggtga tcaaagcggc gcgtaaggcg ctggcggcca
     4921 tgcgagtaag tgtcgaagaa gcgtaatatc aaatcggatg gcgatacgac gtcgccatcc
     4981 gatcttttta tacgacgcgt acgcccgcag gcatcatccg ctccggcgtt aacagcacgc
     5041 tttcactgcc gtcatcggtt tccgcacaca acagcatgca ttccgacgtt tcaccacgca
     5101 ttttcgcttt ttgcagattg cataagacca ccaccgtttt tcccatcagc tcctcttcgc
     5161 tgtagtacgg caccaggctg gtcacggttt gcagtgtttt ttcccccaca tcaacctgta
     5221 cgatgtacag cttgtcggcg ttttcatggc gtttcacttc cacaatcttt ccgacgcgca
     5281 tttccagacg tgcaaaatca gcgtaagcca cggtttccat tgcttcctcc cttcaagtaa
     5341 aattttacta aactatagca aaattttttc tcaatcctgt aggctaaaaa tggagaatgc
     5401 aggcgtgatc acattcgtaa gccgctgtgt taccgttaca gcgtcaaaga aacgcgcttt
     5461 atttactgaa aacaggtgac ccgataagca cttcctctac aatgggggcg cacatcaggg
     5521 aaagtaaaaa aggtaaacat ggcaacatta aaagacatcg caatcgaagc tggcgtatcc
     5581 ctggcgacag tatccagggt cttaaatgac gatccgacat tgaatgtgaa agaagagacg
     5641 aaacatcgca ttctcgagat cgccgaaaag ctggagtaca agaccagtag cgcccgtaaa
     5701 ctacagacag gcgcagtcaa ccaacaccat attctggcta tctacagcta ccagcaggag
     5761 ctggagatca acgatcctta ctatctggcg atccgccacg gcattgaaac tcagtgcgaa
     5821 aagctggcga tcgaactgac caactgttat gaacacaacg gattaccaga cattaaaaac
     5881 gtcaccggta ttttaattgt cggcaaaccc acgcccgccc tgcgcgccgc tgccagcgcg
     5941 ttgaccgaca atatctgttt tatcgacttt cacgaacccg gcagcggtta cgatgcggtg
     6001 gatatcgatc tggcacgcat cagtaaagaa atcatcgact tctatatcaa ccagggcgtt
     6061 aatcgtattg gttttattgg cggtgaagat gagcctggca aggcggatat tcgtgaggtc
     6121 gcctttgcgg aatatggccg actgaaacaa gtggtacgcg aagaggatat ctggcgcggc
     6181 ggtttttcca gttcgtcggg ttatgaactg gcaaaacaaa tgctggcgcg ggaagactat
     6241 ccgaaggcac tgtttgttgc ttccgattcc attgctatcg gcgtactgcg ggcaattcat
     6301 gaacgtggcc tgaacatccc acaggatatt tcgcttttca gcgttaacga tatccccacc
     6361 gcgcgattta cctttccgcc gctctccacc gtgcgcatcc attccgagat gatgggaagt
     6421 cagggcgtta acctggtgta tgaaaaagcc cgcgatggtc gcgcgctgcc gctgctagtc
     6481 ttcgttccca gcaaattaaa actgcgcggc acgacccgtt aaatcccctt acacactgtc
     6541 cggcaatcat ttttgccgga cag
//
LOCUS       DAJVQG010000184         6542 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJVQG010000184 DAJVQG010000000
VERSION     DAJVQG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.184"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..1126)
                     /gene="hemX"
                     /locus_tag="OQM83_003596"
     CDS             complement(<1..1126)
                     /gene="hemX"
                     /locus_tag="OQM83_003596"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCS1521764.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEA"
     gene            complement(1148..1888)
                     /gene="hemD"
                     /locus_tag="OQM83_003597"
     CDS             complement(1148..1888)
                     /gene="hemD"
                     /locus_tag="OQM83_003597"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCS1521765.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            complement(1885..2826)
                     /gene="hemC"
                     /locus_tag="OQM83_003598"
     CDS             complement(1885..2826)
                     /gene="hemC"
                     /locus_tag="OQM83_003598"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCS1521766.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            3213..5759
                     /gene="cyaA"
                     /locus_tag="OQM83_003599"
     CDS             3213..5759
                     /gene="cyaA"
                     /locus_tag="OQM83_003599"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCS1521767.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(5799..6119)
                     /gene="cyaY"
                     /locus_tag="OQM83_003600"
     CDS             complement(5799..6119)
                     /gene="cyaY"
                     /locus_tag="OQM83_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCS1521768.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
BASE COUNT         1475 a         1720 c         1707 g         1640 t
ORIGIN      
        1 tagcttccgt tgtccccgct gccggttgtg ccagcaggtt acgcacgcgg gtctgcatca
       61 gtttttccag catcgcctgg ctttgcaggg tttccggaag atccatcgag atattttgct
      121 ggcttaactg gtccacctcg tcgaggaacg ctttggtggt ggcatcatca gtatcgtagt
      181 aagcacgtac ccaggtggag acgttctcca gcgcctggcg ataagtctct tcctgatgac
      241 gcggtacagc ttgtgctgcg accagcaggc gagagcgaat attttcgcgc agatagatat
      301 cctgatttgg cgctaacagc ggtacggcgg tgtcatcacg acggcgaatc gtaatgaagt
      361 tgtccataaa gttctgccag cttttttgca gattgatacg ccattcgctg atggaactgg
      421 aaagctcttc accgtctgaa tccatcggcg aaccatcgct gtcattatcg gccagacgca
      481 ggttatctac ctgatttgaa agctgattaa gcttaaggat gatgccgtca taatccacct
      541 gcgatactgc agaaaggctg gcgatatcat cggtaattgc ccgacgaacg gtaatcagac
      601 tcgggtcatt catatccgcc aggctggcgt ctgcactttt cagcaacgct gcagcggtcg
      661 tgacgtcctg atcgctccac agcttccgtc cggcgagttt caccagaaaa tcggcctggg
      721 ccagcagcca ggttttagca tcgctgccgg aaatggtggc gaccttttgc tggacttcat
      781 ccagctgttt tgccagcgtt tcttgctgac gattcgcctg ttcaagctgc gtagcctgtt
      841 gcttaataat gccttccagt tcggcttttt ggctctcctg ggctttttgc aatgccgtca
      901 gttggttagc cagggcatcg ctggtggcgg tctgattgac ggcctgttgt ttaccccagc
      961 catacaaacc gacgcccgcc gccagagcaa tagcgatagc cgccgcgctg agaatcaatg
     1021 cggtattgtt cttactcttt ttttctgttg cgacaggttg tgacgtggtg tccacggcct
     1081 ccctagtctc ttcaaccacg gcggaggttt tttcttgttc cgtcattatg gcttcctgtt
     1141 atgagagtta ttgtaatgcc cgtaaaagcg catcgttggc agcgttatcg gcgaccttaa
     1201 tgtcttgcca gcccagttcc cgggcgagtt tcgccaaacg ctcactgacg accaatagtc
     1261 gacagtgtag taaccagtgc tcacgatacc attgtgggat cagcgaccag agttgctgca
     1321 acatttcacc gctggtaaca acgaccgtcg tcacctcgcg ggattgccag cgcatcgctt
     1381 cttctgcacc atcgtaatgg attgcgcatc gttgataaca ttcacaaaaa gtgacctcag
     1441 caccgcgcgc cgtcagggta tccccaatta gctcacgacc gccattgcca cgtaatatca
     1501 gcgcacgttt gcccgcaata ttttgtaatt caggtaattg tagcaagact tcgctgattt
     1561 cccgatcctg cgggtagaga atcttctgtc cgcttacggt atgtagtgcc agtgcggtgg
     1621 tgcgtccaat ggcgaaataa ttaggtagtc ggggccattt acgatcttgc tgatgtagct
     1681 gtgattgggc aaaagcaacc gcgtgttgcg agagggcaaa caacagatcg ctctccccca
     1741 gcgctgccag ttgatcagca agttgcggta attgttgacc cggagaaaac tcaatcagcg
     1801 gaaaatgcca ggccacctgc cccagtgtgc gcagacggct cactaactct tctccagcgg
     1861 gagacgggcg ggtgacaagg atactcatgc cggggcgtct ccgttataga cttcagcgag
     1921 gatctcgcgc gcgccgttat tcagtagctc ttctgccagc gaaatcccca tttgtttggc
     1981 attttgcggc gcaccgcggc gttcaccgcg aataatctgc gaaccgtccg gcgcgccgac
     2041 cagcgcacgc agccagattt cgccatcaat aagctcggcg tagctaccaa ttggcacctg
     2101 acatccgcct tcgagacggg tattcatggc gcgttctgcg gtaacgcgca gtgcagtttc
     2161 gtggtgattc agcgcggcaa gcagctcgcg agtgcgcgta tcatcaaggc ggcattcaat
     2221 accgactgca ccttgtccta ccgccggaag agaaatctcg ggtggtaacg cggcgcgaat
     2281 gcgggactcc agacctaaac gttttagtcc ggctacggca agaatgatgg catcgtattc
     2341 gccgttatct agtttgctca ggcgagtgcc gacgttgccg cgcagggagc ggataatcag
     2401 atccgggcgg cgttcagcca gttggcactg gcgacgtaaa ctggacgtcc cgacgatact
     2461 gcctgctggt aacgcatcca gactgtcata gttattggac acaaaggcat cgcgaggatc
     2521 ttcacgctca caaatagtga ccagtcccag accttgcggg aattcaaccg gcacatcttt
     2581 cattgaatgt acggcgatat cggcgcgatt ttcgaggagc gcgacttcca gctcttttac
     2641 aaataagcct tttccgccta ctttcgccag cggcgtatca agaatcacat cgccgcgcgt
     2701 caccatcggt accagttcaa cgaccaggcc cggatggctc gccatcaact tgtctttgac
     2761 atagtgtgcc tgccagagtg caagtgggct ttggcgtgtg gcaattctta aaacattgtc
     2821 taacatgctt gttaccgtca ttatcatccg tggtccatcc taacatcctt gccagagtga
     2881 tgtcagtgtt gtggtgaaac gtagacgcct gcgcaaaccg taaaatgagg tctggcagtg
     2941 gatcctgaca gacgtttcac gctgtcgtaa taaggaattt acagagaata aacggtgcta
     3001 cacttgtatg tagcgcatct ttctttacgg tcaatcagca aggtgttaaa ttgatcacgt
     3061 tttagaccat tttttcgtcg tgaaactaaa aaaaccaggc gcgaaaagtg gtaacggtta
     3121 cctttgacat acgaaatatc ccgaatgccg cgtgttaccg ttgatgttgg cggaatcaca
     3181 gtcatgacgg gtagcaaatc aggcgatacg tcttgtacct ctatattgag actctgaaac
     3241 agagactgga tgccataaat caattgcgtg tggatcgcgc gcttgctgct atggggcctg
     3301 cattccaaca ggtctacagt ctactgccga cattgttgca ctatcaccat ccgctaatgc
     3361 cgggttacct tgatggtaac gttcccaaag gcatttgcct ttacacgcct gatgaaactc
     3421 aacgccacta cctgaacgag cttgaactgt atcgtggaat gtcagtacag gatccaccga
     3481 aaggtgagct tccaattact ggtgtataca ccatgggcag cacctcgtcc gtagggcaaa
     3541 gttgttcctc tgacctggat atctgggtct gtcatcaatc ctggctcgat agcgaagagc
     3601 gccagttact acaacgtaaa tgcagcctgc tggaaagctg ggccgcctcg ctgggtgtgg
     3661 aagtcagctt cttcctgatt gatgaaaacc gctttcgtca taatgaaagc ggcagcctgg
     3721 ggggcgaaga ttgtggctcc acccagcata tactgctgct tgacgaattt tatcgtaccg
     3781 ccgtgcgtct cgccggtaag cgtattctgt ggaatatggt gccgtgcgac gaagaagagc
     3841 attacgacga ctatgtgatg acgctttacg cgcagggcgt gctgacgcca aatgaatggc
     3901 tggatctcgg tggcttaagc tcgctttctg ctgaagagta ctttggtgcc agcctttggc
     3961 agctctacaa gagtatcgat tccccataca aagcggtgct gaaaacactg ctgctggaag
     4021 cctattcctg ggaatacccg aacccacgtc tgctggcgaa agatatcaaa cagcgtttgc
     4081 acgacggcga gattgtatcg tttggcctcg atccatactg catgatgctg gagcgtgtta
     4141 ctgaatacct gacggcgatt gaagatttca cccgtctgga tttagtacgt cgctgcttct
     4201 atttaaaagt gtgcgaaaaa ctcagccgtg aacgcgcctg tgtaggctgg cgtcgcgcag
     4261 tgttgagcca gttagtgagc gagtggggtt gggacgaagc tcgtctggca atgctcgata
     4321 accgtgctaa ctggaagatt gatcaggtgc gtgaggcgca caacgagttg ctcgacgcga
     4381 tgatgcagag ctaccgtaat ctgatccgct ttgcgcgtcg caataacctt agcgtctccg
     4441 ccagtccgca ggatatcggc gtgctgacgc gtaagctgta tgccgcgttt gaagcattac
     4501 caggtaaagt gacgctggta aacccgcaga tttcacccga tctctcggaa ccgaatctga
     4561 cctttattta tgtgccgccg ggccgggcta accgttcagg ttggtatctg tataaccgcg
     4621 cgccaaatat tgagtcgatc atcagccatc agccgctgga atataaccgt tacctgaata
     4681 aactggtggc gtgggcatgg tttaacggtc tgctgacctc gcgcacccgt ttgtatatta
     4741 aaggtaacgg cattgtcgat ttgcctaagt tgcaggagat ggtcgccgac gtgtcgcacc
     4801 atttcccgct gcgcttacct gcaccgacac cgaaggcgct ctacagcccg tgtgagatcc
     4861 gccatctggc gattatcgtt aacctggaat atgacccgac agcggcgttc cgcaatcagg
     4921 tggtgcattt tgatttccgt aagctggatg tcttcagctt tggcgagaat caaaattgcc
     4981 tggtaggtag cgttgacctg ctgtaccgca actcgtggaa cgaagtgcgt acgctgcact
     5041 tcaacggcga gcaatcgatg atcgaagccc tgaaaactat tctcggcaaa atgcatcagg
     5101 acgccgcacc gccagatagc gtggaagtct tctgttatag ccagcatctg cgcgggttaa
     5161 tccgtactcg cgtgcagcaa ctggtttctg agtgtattga actgcgcctt tccagcaccc
     5221 gccaggaaac cgggcgtttc aaggcgctgc gcgtttctgg tcaaacctgg ggcttgttct
     5281 tcgaacgcct gaatgtatcg gtacagaagc tggagaacgc catcgagttt tatggcgcta
     5341 tttcgcataa caaactgcac ggcctgtcag tgcaggttga aaccaatcac gtcaaattac
     5401 cggcggtggt ggacggcttt gctagtgaag ggatcatcca gttcttcttc gaagaaacgc
     5461 aagacgagaa tggctttaat atctacattc ttgacgaaag caaccgggtt gaggtgtatc
     5521 accactgcga aggcagcaaa gaggaactgg tgcgtgacgt cagtcgcttc tactcgtcat
     5581 cgcacgaccg cttcacctac ggctcaagct tcatcaactt caacctgccg cagttctatc
     5641 agattgtgaa ggttgatggt cgtgaacagg tgattccgtt ccgcacaaaa tctatcggta
     5701 acatgccgcc tgccaatcag gatcacgata cgccgctatt acagcaatat ttttcgtgat
     5761 gaacgtgccg gaaagcgagg cttatccggc atgcaatctt agcggaaact gactgtttca
     5821 cccgcctgct gcgtcgccgc ctgttccagc aaatcccaga aggtttcgcc gctgcgatcg
     5881 caaatccact catcgccttt cagatcaaaa tggtagccgc cttgtttggt tgccagccat
     5941 acctggtgca gcggttcctg gcggttgata atgattttgc tgccgttttc aaaggtaatg
     6001 gtcagcacgc cgccgttgat ttcgcagtcg atatcgctgt cgccatccca gtcgtccagg
     6061 cgttcttcaa tggtcagcca gagttgatca gccaggcgat gaaattcact gtcgttcatt
     6121 gttgtatcct gtttttaagt gatggcggca gtatagcggc atggggtcag ggcttcaaag
     6181 tttgcacctc tgcggctgcg ttccggcacg attcatccgt caccggaata atgatgtctc
     6241 tgtgtagcga aagatttgtc tcttcattag ggcgcagtta caccacgcct ttccctgttt
     6301 ctggtaaaca ttatgatcag gtttaccgag cgagcatcct cacgctgacg gaactaaaaa
     6361 agacaacaaa caaaccacat tgcgatagtg catagagcca tcctggcgcg aggtgccgat
     6421 cacgaaacta ccagcaaaac ataaatcccc acgagtaagc gttatactcg cagcatttcc
     6481 tcacttttca gacttcataa agagtcgcta aacgcttgct tttacgtctt ctcctgcgat
     6541 ga
//
LOCUS       DAJVQG010000185         6537 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJVQG010000185 DAJVQG010000000
VERSION     DAJVQG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6537
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.185"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            142..1317
                     /gene="mdtL"
                     /locus_tag="OQM83_003601"
     CDS             142..1317
                     /gene="mdtL"
                     /locus_tag="OQM83_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCS1521769.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1292..2251
                     /gene="yidZ"
                     /locus_tag="OQM83_003602"
     CDS             1292..2251
                     /gene="yidZ"
                     /locus_tag="OQM83_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCS1521770.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2408..3157
                     /locus_tag="OQM83_003603"
     CDS             2408..3157
                     /locus_tag="OQM83_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCS1521771.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3179..3729
                     /gene="yieF"
                     /locus_tag="OQM83_003604"
                     /gene_synonym="chrR"
                     /pseudo
     CDS             3179..3729
                     /gene="yieF"
                     /locus_tag="OQM83_003604"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
     gene            complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="OQM83_003605"
     CDS             complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="OQM83_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCS1521772.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5286..5951
                     /gene="yieH"
                     /locus_tag="OQM83_003606"
     CDS             5286..5951
                     /gene="yieH"
                     /locus_tag="OQM83_003606"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCS1521773.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6018..6491
                     /locus_tag="OQM83_003607"
     CDS             6018..6491
                     /locus_tag="OQM83_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCS1521774.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1607 a         1650 c         1652 g         1628 t
ORIGIN      
        1 atttggctaa atccttcaag aagccagcca ttcgctggct tcttgcctct caggaaatca
       61 cttatgtcca aatggcaact cgcctgatcc tccttcacca cgtatgcttt gcgtcacctt
      121 actatcagga cgctttagcc catgtcccgc tttttgattt gtagttttgc cctggtttta
      181 ctttatcccg ccgggattga tatgtacctc gttggtttac cgcgcatcgc cgccgatctc
      241 aatgccagcg aagcgcagtt gcatattgcg ttctccgtat atctggcggg gatggcagct
      301 gcgatgttat ttgccggtaa agtggccgat cgttcaggga gaaagccggt cgccataccc
      361 ggcgcggcgc tatttattat tgcctcggtg ttctgttcac tggctgaaac cagcacgtta
      421 tttcttgcag gccgatttct acaggggttg ggcgcaggct gttgttacgt agtggcgttc
      481 gccattttgc gcgacacgct ggatgatcga cgtcgggcta aagtgctgtc attactcaac
      541 ggtattacct gcatcattcc ggtgttagcg ccagtgctcg gacatctgat tatgcttaaa
      601 ttcccgtggc agagtctgtt ctgggcgatg gcaatgatgg gcatcgcggt actgatgttg
      661 tctttgttta ttttaaaaga aacgcgccca gcgtcccccg ctgcttcgga caaaccacga
      721 gaaaatagcg agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc
      781 agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg tattgctgat ggaaatcatg
      841 ggttttgagc gcggagaata cgccaccatt atggcgttga ccgctggcgt cagcatgacc
      901 gtttcattct ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc
      961 tcgcaggtgt tattccttgc ggcggggatc acccttgccg tttcaccttc ccatgcggtt
     1021 tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg
     1081 agtcaggcgt taggaccatt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt
     1141 gcgcaggttt gcggttcgtc actgtggatt tggctggcag cggtggttgg tattggcgca
     1201 tggaatatgc tgatcgggat tctgattgcc tgtagcatag tgagcctgtt gctgattatg
     1261 ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa tccatcacca cgcttgatct
     1321 caatctgctg ctctgtctgc aactgctgat gcaggagcgc agcgtgacca aagcggcgaa
     1381 gcggatgaac gtgacccctt cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt
     1441 tgacgacccg ctctttgtga actcaccgct gggcctgtcg cccacaccgc taatggtcag
     1501 catggagcaa aatctggcgg aatggatgca aatgagcaat ctgctgctgg ataaaccgca
     1561 ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg gaatcgccgc tgatgatgat
     1621 catgcttaat gcgctgtcga aacggatcta ccaacgttac ccgcaggcga ccatcaaact
     1681 acgtaactgg gattacgatt ccttagatgc cattactcgt ggtgaagtgg atattggttt
     1741 ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta agctcgctac cgttagccat
     1801 tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc
     1861 actgcatgaa acgtggaatc tggacacctt tttacgttat ccgcatatca gcatttgctg
     1921 ggaacagagc gacacctggg cgctggacaa tgtgttacag gagctggggc gcgaacgcac
     1981 gattgctatg agtctgccgg aattcgagca gtcactgttt atggcagcgc aacccgacaa
     2041 tctgctactg gcgaccgcgc cgcgctactg tcagtactac aatcaactcc atcaactgcc
     2101 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
     2161 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
     2221 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
     2281 acaataaaat gtaaaaaagt tgtaacaagc ttgtctgaat cgaactttta gccgctttag
     2341 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
     2401 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
     2461 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
     2521 ttttctggcg tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
     2581 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
     2641 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
     2701 atgtggcctc gatatggaac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
     2761 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
     2821 cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
     2881 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
     2941 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
     3001 ggtcacgccc gcgattgaaa agctcagtgt ctgggagtta gatggcaaac acagctggaa
     3061 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
     3121 ccagctctct accgtgaagt ctttttcgcc aaattgatag acaaccacag gagtcattat
     3181 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
     3241 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
     3301 ttccattgcc gacattcccc tgtatgacgc tgacgtacag caggaagaag gttttccagc
     3361 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
     3421 ggaatataac tactctgtac cgggtgggct gaaaaatgcc atcgactggc tttcccgctg
     3481 gccggtaaac cggtgttgat tcagaccagc tcaatgggcg tgattggcgg cgcgcgctgt
     3541 cagtatcacc tgcgccagat tctggttttc ctcgatgcaa tggtgatgaa caagccggaa
     3601 tttatgggcg gcgtgattca gaacaaagtt gatcggcaaa ccggagaagt gattgatcag
     3661 ggtacgctgg accacctgac cgggcaattg accgcatttg gtgagtttat tcagcgagtt
     3721 aagatctaaa taaaaaaccc gccagtaatc atgcatggcg ggtttttaat gcgctatcga
     3781 ttttaatgag cgtcgataaa tacaatcttc aggataaaca gcagcgcaac gatgattacg
     3841 cacgggctaa ggtcacgcag acgcccggta ccaatcttca tcacgcagta ggagataaag
     3901 cccagcgcaa taccttcggt aatcgaaaag ctgaacggca tcatcacggc ggtaataaac
     3961 gccggaacag attcagtaag atcctgccag ttcacgcgag caagacttga ggtcatcaac
     4021 acgccaacgt agatcaacgc gccagctgca gcgtagcctg gcaccattcc cgccagcggc
     4081 gacagaaaga taaccagcag gaacaacaga ccaacaacca ctgccgtcag accggtacga
     4141 ccgccaaccg atacgccgga agaggactca atataagccg taacggaaga agtaccgata
     4201 aacgaaccgg tcacggaaga gatactgtcg acatacagcg cctgcttcat gcgcgggaat
     4261 ttccccttct catccgccag acctgcttta tcggtcacgc caatcagcgt accggaggag
     4321 tcaaacaagt tgaccaacat gaaagagaaa atcacccctg ccagcccgag gttaaacgac
     4381 ccggctaaat ctacatgacc cacaactgtc attacgctcg gcggcgcaga aacgatgcca
     4441 ttgtagtgca catcacccag catccagccc agcagcgtcg tcaccacgat agaaaccagc
     4501 accgctgcgt gaatgttgcg cgaggccaga atggcgatga tgaagaaacc gaggatcccc
     4561 agcagcacgc tgtgagaagt cagattaccg atgctcacca gcgtttccgg gttagcgaca
     4621 atcacacctg cgtttttcag ccccatcatg ccaatgaaca gaccgatacc gctggtaata
     4681 cccacacgca gacttaccgg aatgttagca atcatccagt aacgaacgcg gaaaatggtc
     4741 aaaagaagca gtccgacagc gccccagaag attgcgccca taccgatctg ccacggcagc
     4801 cccattgcct ggacaaccac aaaagcgaaa aaggcgttaa gccccatcgc aggagccagc
     4861 gccaccggaa ggtttgcaaa caatcccatc ataatgctac cgaaagcagc aatcagacag
     4921 gttgttacga agacggcact ggtgtccatg ccagcaacgc ccagaatttg tgggttaaca
     4981 aatacgatgt agaccatcgt caggaaggtg gtgaacccgg caatcacttc agttcgtgcc
     5041 gtagtaccat gttcgcgcag tttaaaaact cgttccagca tcccctgacc agaagtctgg
     5101 gtggtatgtt gatgactcat tatctatttc cgaacaagga gggaaaatcc gtcgctatcg
     5161 tataccaaaa tgcgacaata ggcgcgtttg tgagagactt tttttattgg atttacttat
     5221 acggcaacga ttgcgttgcg caaatcggct ctacgaaaac gttaaactga ttaaaaagga
     5281 aaggcatgtc ccggatagaa gcggtatttt tcgactgcga cggtacgctg gtcgacagtg
     5341 aagtcatttg ctcccgcgca tatgtaacga tgtttcagga atttggtatt acgctcgatc
     5401 ctgaagaggt attcaaacgt ttcaaaggtg taaaactgta cgaaattatc gatattgttt
     5461 cccttgaaca tggtgttacg ttagcgaaaa cagaatctga acacgtttac cgtgcagaag
     5521 tcgctcggct gttcgattca gaactggaag ccatcgaagg ggctgaagcg ctcctgtcag
     5581 cgatcactgc gccaatgtgt gtggtatcta acggcccaaa taacaaaatg cagcattcta
     5641 tgggcaagct gaatatgttg cactacttcc cggataaact gttcagcggc tacgatattc
     5701 agcgctggaa gccagacccg gcgttaatgt tccatgcggc aaaagcgatg aatgtaaatg
     5761 tagaaaactg cattctggtt gatgactcag ttgccggtgc gcaatctggt atcgacgcag
     5821 gtatggaagt gttctacttc tgcgccgacc cgcacaataa gccgatcgtt cacccgaaag
     5881 tcaccacctt tacccatctt tcgcagttac cggaactgtg gaaagcgcgt ggttgggata
     5941 ttacggcata gttcttcaca ctcccttcac ttaccccgct taaattggcg ctcaaaggta
     6001 agtaaaggga gtttgatatg tctgtttcac gtcgggtaat acatcacgga ctttattttg
     6061 cggttttagg accgttaatt ggtgttctgt ttcttgtcct ctacatattc ttcgcaaaag
     6121 aaccgctggt tcttttggta ataatacagg tacttccatt gtttctctta ttgtcgatta
     6181 ctacgggagc tattcctgca ttgttaaccg gcgtgatggt cgcctgtctg ccggaaaaga
     6241 tcgggtcaca gaaaaattat cgttgtctgg ctggtggcat aggtggcgtc gttatcaccg
     6301 agatctattg tgcagttatt gtacatatta agggcatggc ttcctcggag ttgtttgaaa
     6361 acattctttc tggtgacagt ctcgttgtcc gcatcattcc tgcattgctg gcaggtgtgg
     6421 tgatgagcag aatcattacc cgtctgcccg gattggatat ttcatgtcct gaaacagact
     6481 ctttaagtta agcgggatac tttatctttg ggctactcaa aagcagacag gatgtta
//
LOCUS       DAJVQG010000186         6512 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJVQG010000186 DAJVQG010000000
VERSION     DAJVQG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6512
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.186"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(2..378)
                     /gene="rnpB"
                     /locus_tag="OQM83_003608"
     ncRNA           complement(2..378)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OQM83_003608"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(411..1556)
                     /gene="garK"
                     /locus_tag="OQM83_003609"
     CDS             complement(411..1556)
                     /gene="garK"
                     /locus_tag="OQM83_003609"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCS1521775.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1653..2543)
                     /gene="garR"
                     /locus_tag="OQM83_003610"
     CDS             complement(1653..2543)
                     /gene="garR"
                     /locus_tag="OQM83_003610"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCS1521776.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2573..3343)
                     /gene="garL"
                     /locus_tag="OQM83_003611"
     CDS             complement(2573..3343)
                     /gene="garL"
                     /locus_tag="OQM83_003611"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCS1521777.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRTNMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3359..4693)
                     /gene="garP"
                     /locus_tag="OQM83_003612"
     CDS             complement(3359..4693)
                     /gene="garP"
                     /locus_tag="OQM83_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCS1521778.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5068..>6512
                     /gene="garD"
                     /locus_tag="OQM83_003613"
     CDS             5068..>6512
                     /gene="garD"
                     /locus_tag="OQM83_003613"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCS1521779.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHTPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEES"
BASE COUNT         1590 a         1795 c         1610 g         1517 t
ORIGIN      
        1 caggtgaaac tgaccgataa gccgggttct gtcgtggaca gtcattcatc taggccagca
       61 atcgctcact ggctcaagca gcctacccgg gttcagtacg ggccgtacct tatgaacccc
      121 tatttggcct tgctccgggt ggagtttacc gtgccacgga ctgttaccag ccgcgcggtg
      181 cgctcttacc gcaccctttc acccttacct gatcccgctt gcgcgggcca tcggcggttt
      241 gctctctgtt gcactggtcg tgggtttccc ccccaggcgt tacctggcac cctgccctat
      301 ggagcccgga ctttcctccc ctccgcccgt ctcccccgaa gaggacgacg acgaagcggc
      361 gactgtctgg tcagcttcgg cgcgcagtat agagggtttg cgcgcccttg tcaccccgcg
      421 ttgcgcattc caatcgccag tgtcgcggcg atattacgtg aagcacggca gatattgtca
      481 taagccccgc ggaatgcttc gtccaacgta cctatgctgg tcaatacgct gaagaccgca
      541 tcgatgccat gctgatgtac aacgccaaca tcatcggtca ggctacccgc aatgccaatc
      601 accggtttat ggtacttctt cgccacgttt gcaacgccaa tcggtacctt gccgtgaata
      661 ctctggctgt caatacgccc ttcaccggtg atcaccagcg tacaatcgtg aatatgttct
      721 tccagattca gcgccgtagt gacgatttca ataccacttt tcagttccgc accaagaaac
      781 gccatcagcg ccgcgcccat accgcccgca gctcctgcgc cggggacatc tttcacatca
      841 acatgcagcg cttttttaat gacatcggca tagtgagaga ggttattgtc cagctcaaca
      901 atcatcgctt cactggctcc cttttgtggg ccaaagatgc gcgatgcgcc gttatcgccc
      961 accagcggat tggtgacatc acaagcgacg cgaatgacgc aatcttttaa gcgcggatcg
     1021 aggccggaaa tatcaatatc attcagagta ttaagactac cgccgccaaa accaatttca
     1081 ttgccgttgg cgtcgcataa tttcgccccc agcgcctgta ccatgcctgc gccgccatca
     1141 tttgtagcgc tgccgccaat gccgataata atgtttgttg cactgctctc cagcgcctgc
     1201 aggattaact cgcctgtgcc gcgtgaagtg gttacgagtg gatcgcgttt ttccgcaggt
     1261 accagctcca gcccactggc cgccgccatt tcaataaacg cggttttgcc atcgccggag
     1321 atcccccaac tggcattcac tttctcgccc agcggccctg taacccaggc gtgacgttca
     1381 gccccctggg tggctgcaat catcgcttcc accgttcctt caccaccgtc ggcaaccgga
     1441 acagaaacat actgtgcatc aggaaaaatt tcccgaaatc ctttttctat cgcctgcgca
     1501 acctcgctgg cagataaact ttctttataa gagtctgggg cgattacgat tttcatacct
     1561 atgcctgtta ccacatgacg ccggagggcg tttctcttat tcggcctaga ttccaggccc
     1621 ggattgcaat acaccatccg ggcacgacgt cattaacgag taacttcgac tttcgccagt
     1681 ttttcgtagt agcacgccag ggcgctatga tccgccgttc ctaaaccatc tgcacgcagt
     1741 gcctgcatca tctccataac cgcagctgtg agcagcagtt gtgcgccgac gccgtgagaa
     1801 gtatccagcg cattcgccag atccttaata tgcagatcaa tgcggaagcc cggcttgaag
     1861 ttgcggtcca tcaccatcgg cgctttggca tccagcacgg tactgcccgc cagcccaccg
     1921 cgaattgcct gataaaccag gtccgggtta acgcccgctt tggttgccag cgttaacgct
     1981 tctgacatcg cagcaatatt cagcgccaca atgacctgat ttgccagttt ggtgacgtta
     2041 cctgcaccga tttccccggt atgcaccacg gaacccgcca tcgctttcat caaatcatag
     2101 tatttgtcga aaatagcctt gtcgccgccc accatcactg acagcgtacc gtcgatggct
     2161 ttcggttcac cgccgctcac cggagcatcc agcatatcaa tgcctttcgc tttcagcgct
     2221 tcgctgattt cacggcttgc cagcggtgcg atagaactca tatcgatcaa taccgtacct
     2281 ggcttcgcgc cttcaataat gccattctca cccagcgcca cctctttcac atgaggggag
     2341 tttggcagca tggttatgat gacgtcgcac tgttcagcga tcgctttagc cgtagacgct
     2401 gtttctgcac ctgcagcaat cacgtcagca atagcttctg ggttacggtc agcaaccacc
     2461 agcgagtaac ctgctttcag aaggttttta ctcattggtt tacccataat ccccaggcca
     2521 ataaaaccaa ctttcatagt catatcaacc atctctcttg ttgcggtggt ggttattttt
     2581 taaaggtatc agccagtttc tgagtggcag agcggaagac gccaagatcg ctgccgacag
     2641 ccacaaacgt cgcgccccat tccagataac gacgcgcatc ggcttcgacc ggcgcgagaa
     2701 taccgctggg tttgccgtgc gcgctggcac ggttaaaaat atgctgaatt gctttttgta
     2761 catccggatg cgaggcattg ccgagatggc ccaatgccgc agccagatcg ctggggccga
     2821 cgaagatacc gtctacacct tcggtagcgg caatagcatc gacgttatct acgccctgct
     2881 gactttctat ctggaccaga atagtaatgt tcttgttcga ctgagcgaaa taatccgcca
     2941 cggtgccaaa catattggtg cggtgagaaa cggagacgcc gcgaatgcct tccggcgggt
     3001 aacgagttga tgccaccgcc tgctctgctt cctcttttgt ttctacaaaa ggaatcagga
     3061 agttatagaa accgatatcc agaagacgct taataattac cggctcgttg gtcggcactc
     3121 gcactactgg cgcgctggcg ctgcctttca gggccattaa ctgcggaata aacgtggaaa
     3181 tatcgtttgg cgcatgttcg ccatccagca ccagccagtc aaacccagcc aaaccaagaa
     3241 cttcagtgct aatcgggtta gagagtgctg accagcaacc aatttgtacc tgtttcgcag
     3301 ccagtgcggc tttgaattta ttcgggaaaa catcgttatt catcgcttat acctttgctt
     3361 atttctgcaa ttccatacgt ttaatgtcgc caactacgaa gaggtagcag accatcgcca
     3421 tcagcgctga acatcccacg aaaaccagtg ctgcattgaa ggagtgcagt tcacttacca
     3481 ggtagccaat caccagtgga gtgacaatgg aggcaacatt gccaaagacg ttaaagacgc
     3541 cgccacagag gccaacaatc tctttcggcg cggtgtcaga aatcaccggc cagcccagag
     3601 caccaaatcc tttgccaaaa aaagccagcg ccatcagcat gaccaccagc gtggtgttgt
     3661 tggtgtagtt acataagatg atggtggaaa ccagcaacat tcccagcaca atcggtagct
     3721 tacgtgccag ggtcagggat aaaccgcgtt tgatcagata atccgagaag acacctccca
     3781 gcacgccgcc cgcaaaacca cacagtgccg gaatcgaggc gaccagaccc actttcagaa
     3841 tcgacatgcc tttttcctgc accagataaa tcgggaacca ggtgaggaag aaccaggtga
     3901 tggtgttgat aaaatattgt ccgaaaaata cgcccaacat catgcggtta gagagcaatt
     3961 gcttgatgta atgcagtttg ggaccgcttg ctgccgcgct gcccggcttt ttgtggtcca
     4021 tatcgaccac cgcgccattt tcagagataa acttcagctc ttccgcagac atacgtgggt
     4081 gatctgtcgg gttatgaatc aacttgatcc acagcgccgt cagcacaaaa ccaatccccc
     4141 ccataacggt gaagacgtgc tcccagcccc aagcgaaagt cagccagcca agcagcggcg
     4201 aaaagagcgc cagcgagaaa tattgcgccg agttaaagat ggcggaggca gtaccacgtt
     4261 ctttcgtcgg gaaccaggcg gcgacaatac gggcgttcgc cgggaatgat ggcgcttccg
     4321 agaaccccag cataaagcgc ataaagaaca tggagatccc tgcccaggcc agcgggaaca
     4381 tatcaacaaa gccttgcagg aaggtgaaca gcgaccagaa aaagaggctg taggtgtaaa
     4441 cttttttcga gccaaactta tcaagcaacc agccgccggg gatttgcatc agcaagtagg
     4501 cccagccaaa agcagagaag atgtaaccca tcgaaaccgc acttaactgc aactcttttg
     4561 ccacttcggt accagcaata gacagcgttg cacgatcggc gtagttaacg gcggtaacaa
     4621 taaaaataat cagtaatatt aaatagctgg tatgcacgcc tttctttttt acgtcaacgg
     4681 tgtccagaat cattttattt acctcgggta cttatgctga tttttattat tatggggaag
     4741 gtgttattta tgagttttat ttatgccgtt acgacaatga actcgggaat tagtataagc
     4801 agcgggagaa taataatcat tgtgcaaatg ctaatttaat cagtactatt taaatattat
     4861 tttgagcata tgcacataag gttgcgcgct aaagcacaga tttgcgcttt accttaccgg
     4921 gcggcactgc aatccctgaa atgattgaca ttgatcacat ttctgcgttt aaactcctga
     4981 cattcttatt tcacccaatg aagtcattta tttttaaatg agaccagatc ctcattttaa
     5041 taacccctgg ctggagaata ttgcacaatg gccaacatcg aaatcagaca agaaacgcca
     5101 actgcgtttt atataaaagt tcacgacaca gataatgtgg caattattgt taatgataat
     5161 ggcctgaaag caggaacgcg ttttccggat gggctggaat taattgaaca tactccccag
     5221 gggcataaag tcgcattgct ggacattcca gctaatggtg aaattattcg ttatggcgaa
     5281 gtgattggtt acgccgtgcg cgcaatccca cgcggaagct ggatcgacga atcaatggtt
     5341 gtactaccgg aagcgccgcc gttacacacg ctgccactgg caaccaaagt cccggccccc
     5401 ttaccgccgc tggaaggata cacctttgag ggctatcgca atgccgatgg cagcgtgggc
     5461 accaaaaacc tgctcggcat caccaccagc gtccactgtg tggcaggcgt ggtggactat
     5521 gtagtaaaaa tcattgaacg cgatctgcta ccgaaatacc cgaacgtcga tggcgtggtg
     5581 gggctgaatc atttgtacgg ctgtggcgtg gcgattaacg caccggcggc agttgtgcct
     5641 attcgtacca tccacaatat ttcgctgaac cctaactttg gcggcgaagt aatggtgatt
     5701 ggtctgggtt gtgaaaagtt gcagcctgag cgcctgctgg tcggcacgga tgatgtgcaa
     5761 gctattccag tagaaagcgc cagcattgtc agtttgcagg atgaaaagca tgtcggcttt
     5821 cagtccatgg tcgaggatat tttgcaggtc gccgaacgcc atctacaaaa actgaatcaa
     5881 cggcagcgag aaacctgccc ggcttcagaa ctggtcgttg gtgtgcagtg cggtggcagc
     5941 gatgcgtttt ctggtgtaac ggcaaacccg gcggttggct atgcgtctga tctactggtg
     6001 cgctgcggcg caacggtgat gttttcagaa gtaacggaag tgcgtgacgc gatccatctg
     6061 ctgacaccac gcgcagtgaa cgaagaggtc ggcaaacggc tgctggagga gatggagtgg
     6121 tacgataact atctcaatat gggaaaaacc gaccgcagcg ccaacccttc gccgggcaac
     6181 aagaaaggcg gtctggcaaa tgtggtggag aaggcactcg gctccattgc taaatcgggt
     6241 aaaagtgcaa ttgttgaagt gctgtcgccc ggtcaacgcc cgactaaacg cggattaatt
     6301 tacgccgcga cgccagccag cgattttgtc tgtggcacgc aacaggtggc ttcgggtatc
     6361 accgtacaag tgtttacgac cggccgtggt acgccgtacg gcctgatggc ggtacccgta
     6421 attaaaatgg caacccgcac cgagctggcg aaccgctggt ttgatttaat ggatattaac
     6481 gcgggcacta tcgctaccgg cgaagagagc at
//
LOCUS       DAJVQG010000187         6327 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJVQG010000187 DAJVQG010000000
VERSION     DAJVQG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.187"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..356
                     /gene="mcbR"
                     /locus_tag="OQM83_003614"
     CDS             <1..356
                     /gene="mcbR"
                     /locus_tag="OQM83_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCS1521780.1"
                     /translation="TPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNM
                     PILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAE
                     IMQQNIAILYQQYNR"
     gene            complement(392..2494)
                     /locus_tag="OQM83_003615"
     CDS             complement(392..2494)
                     /locus_tag="OQM83_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCS1521781.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2736..3797
                     /locus_tag="OQM83_003616"
     CDS             2736..3797
                     /locus_tag="OQM83_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCS1521782.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3912..5411)
                     /gene="ansP"
                     /locus_tag="OQM83_003617"
     CDS             complement(3912..5411)
                     /gene="ansP"
                     /locus_tag="OQM83_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HCS1521783.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5678..6295
                     /locus_tag="OQM83_003618"
     CDS             5678..6295
                     /locus_tag="OQM83_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HCS1521784.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGSGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
BASE COUNT         1704 a         1594 c         1513 g         1516 t
ORIGIN      
        1 tcaccccgca agacctgacg gaactccagg aattgctcga gaaattacag caggcgcaag
       61 aaaagggcga catggaacaa atcattaatg taaacaggct atttcgctta gcgatttatc
      121 atcgctcaaa tatgcccatc ctgtgtgaga tgattgagca actgtgggtc aggatggggc
      181 ctggtttaca ttatctctat gaagcgatta atccagcgga attacgggag catatagaaa
      241 actatcatct gttactcgct gcgttaaaag caaaagacaa agagggatgc agacattgtc
      301 ttgctgaaat tatgcaacaa aatattgcta ttttatacca gcaatacaat cgttaaagta
      361 aatgcccggc aaataccggg catcaaggta attactcaaa tctccacgca atattcatgc
      421 cgacaccata atttcgtccg ggcgaaggtt cgtaatatcg cccgtttgac tcattgacaa
      481 tgacagaacc aacgtattct ttatcgaata aattatcgac acgaccaaat aaatccacag
      541 ttaaattgtg gtaattgtat ttatacccgg tgaataagcc gacgagagta taagacggcg
      601 cttttgccgt attttcatca tctgccataa tatcgcccat ataacgcgct tccgtgcctg
      661 cataccaacc atcttccggt acataaccta tcgacgcaaa gcccatatta cgggcgatcc
      721 ctggcatccg attaccgtta caatcctgtt cattgcaaac attgctgcga taggtcgcat
      781 caagccaggt ccaggacgcg tttacgcgaa aatctcctgc gaaacgttga tcccatgcca
      841 gttcagcgcc ttgacgacgg gtctttccgg catttttgta agtcgtacgc ccaccgctac
      901 tgctatcgac aacaatttca tcatcagtgt cggtctgaaa caatgcgaga ctaagcagcc
      961 catcaccaat acgcgtttta ctgccgatct caattgtatc gttggtggat ggttttaaac
     1021 caaagttcat accgctttgc ccatcagcac gataagacag ctcattaatc gtcggcgttt
     1081 caaaacctcg cccggctgcc agatagatat tccaggcatc ggtcattgca tattttaacg
     1141 aaccggcagg tagccattta tgataactgg catcaccgct gtcatcgccg ttacccggag
     1201 taacgtaatg gtcgttggaa tcaaaccaca cggagctgta gcgcacgcca gcatccagcg
     1261 acagtttttc gctcagctgc cactgcgtct gtaaataggg atcgatgttc cacatcagat
     1321 tgcgttcgtc gcgacgcaac tcaccttttt gcccgtactc cggcatgccg ctattcaggc
     1381 ggaagttatt gtagcccttg cggttttcac tcatgttttc gtagttcagg ccggtagtga
     1441 acgtgaccgg aacgcccagt tcaccacggt gtgtccagcg gctgtctatc ccctggtaat
     1501 ggcgttgcag ggtaatcacg ccgcccgcat gtgacgggtt aagttgtggt gccatgggta
     1561 ttgactggta ctgggtcgtt tctcgctctc cggcatacat catcacactc atatcatctt
     1621 gcgcgctcag gctacgctca tagcgcaacc cagcctgagt ttgcttgatg gtttttcgcg
     1681 tgtcgtactg ttctgcacga ggcgcttgtt gtggattagc cttccattct gctttggtta
     1741 gcccacccgg gtcatctgct ttgatatcca cactattgaa aatcagactt aatttgctgg
     1801 cttcatcaat gcgtacgccc agtttggcat tggctaaatt tttctgtgcg ccactatggt
     1861 cacgatagcc gtgggtcgta aaacgcgtgg ttgagacggt gtaatcgaca tcgccaggct
     1921 gtgtgccgtc tcccgttgcg cccgttgctt tcagcccata gcgccagctg ccaaaactgc
     1981 cgtagtaact actggcttca atggttggtg gctgttgtcc ggtctgggtg gtgacattca
     2041 ttaccccacc agacgcgttg ccatacaggg cagagaaggg gccacgcagc acttccacat
     2101 tttgcacact gcttaaatcg atgttggatg tttgcccttg cccgtcgggc atggtggcgg
     2161 gaataccgtc cacatacagg cgaataccgc gaataccgta agtggagcgg gagccaaatc
     2221 cgcgaatcga cagctgtaaa tcttgcgcat agttctgccg gttttgtacc tgcaaaccag
     2281 gcacgtcggt cagtgattcg gacaagttaa tgcgcggtgt tgccaggcgc atctcctcgc
     2341 catccaccac gcttactgct gctggggtat ccagttctga aaccacctgc ggtgcggcac
     2401 tgacaatcat ggtctgttca tcagcggcaa aaacaacggg ggaaagggca agtagtgcgg
     2461 gcaaaacggt ctgtcggacg gaaaaaatct tcatgaaaaa agccaggtta agaatgggaa
     2521 aacgccgtta tggtaataaa attgtaaatt catggaaaac gaaacggcac gatacgttaa
     2581 gtaattgaga aaattgtagt cgtaacggca agaaatgctc cacatttgag aaaataatga
     2641 ttaccattcc catttataac aagagcgtaa cgatgattac gcttagcgaa gcattgtgaa
     2701 gcagcaaaaa tatcggttca tcaaagggag tcgtcatgca tttacgtcat ctgttttcat
     2761 cgcgcctgcg tggttcatta ctgttaggtt cattgcttgt tgcttcatca ttcagtacgc
     2821 aggccgcaga agaaatgctg cgtaaagcgg taggtaaagg tgcctacgaa atggcttata
     2881 gccagcaaga aaacgcgctg tggctcgcca cttcgcaaag ccgcaaactg gataaaggcg
     2941 gcgtggttta tcgtcttgat ccggtcactc tggaagtgac gcaggcgatc cataacgatc
     3001 tcaagccgtt tggtgtcacc atcaataaca cgactcagac actgtggttt ggtaacactg
     3061 taaacagcgc ggtcacggcg atagatgcca aaacgggcga ggtgaaaggc cgtctggtgc
     3121 tggatgatcg taagcgtacg gaagaggtgc gcccgctgca accgcgtgag ctggtagctg
     3181 acgatgccac gaacaccgtt tacatcagtg gtattggtaa agagagcgtg atttgggtcg
     3241 ttgatggcga gaatatcaaa ctgaaaaccg ccatccagaa caccggtaaa atgagtaccg
     3301 gtctggcgct tgatagcaaa ggcaaacgtc tttacaccac taacgctgac ggcgaattga
     3361 ttaccatcga caccgccgac aataaaatcc tcagccgtaa aaagctgctg gatgacggca
     3421 aagagcactt ctttatcaac attagccttg ataccgctag gcagcgtgca tttatcaccg
     3481 attctaaagc cgcagaagtg ttagtggtcg atacccgtaa tggcaatatt ctggcgaagg
     3541 ttgcagcacc ggaatcactg gctgtgctgt ttaacccagc gcgtaatgaa gcctacgtaa
     3601 cgcatcgtca ggcaggtaaa gtcagtgtga ttgacgcgaa aagctataaa gtagtgaaaa
     3661 cgttcgatac gccgactcat ccaaacagcc tggcgctgtc tgccgatggc aaaacgctgt
     3721 atgtcagtgt gaaacaaaaa tccactaaac agcaggaagc tacccagcca gacgatgtga
     3781 ttcgtattgc gctgtaatag atatggcgag ggtgaaaacg gagagacttg cctgactttt
     3841 atcacgccgc atgcggcaca ccttcgtcag taatctaaat ttgcccggca cgtagccggg
     3901 caattttgca attagctcgc cgtttcaggc ttaaacacaa tttcctgttt ttcttcatct
     3961 tcctcgacga ctggcgcagt gctgtgaatt tcagcaacgc gtttgcgcac accaaaccag
     4021 cctataacca gcagaatacc gataatcggc agcgccgcga tagtataagt cccgttcggg
     4081 taatcgaacg ccatcagcac aaggacactc agtaaaaaca gtaatgtcag ccaggaagtg
     4141 aagggcgcgc caggcagttt aaaactgaca tccgctgctt tgccttcttt aatcgcttta
     4201 cgcaggcgca tctggcacac gatgataaac gcccatgaag cgatgattcc cagcgacgcg
     4261 aagttcaaca caatctcaaa tacgcgcgac ggcaccagat agttgaggaa tacgccgacg
     4321 acatacacaa ccagtgtcgc cagaatcccg gcatacggca catgctgacg actcattttc
     4381 gccataaaac tcggtgcgga accgcccatc gccatcgagc gcagaatacg cccggtgcag
     4441 tacagacctg agttcaggct ggagagggca gcggtcagca ccacaatgtt cataatgctg
     4501 ccgatatatg gcacacccag tttagagaaa aacgtcacga acggactttg ccccgcctga
     4561 tacgcgctcc acggcaataa cataaccagc aacaccacgg agccgacgta aaacaggcca
     4621 atacgccaaa tcacactgtt aatggcttta ggcaccatgg tctgcggatc tttacattct
     4681 ccggcagctg tacccaccat ttcaatggag gcaaaagcaa acactacgcc ctgaatcaac
     4741 accagcgcag gcagcaaacc gtgggggaag aagccgccat tatcggtgat taaatgaaag
     4801 ccagtggtgt tgccatccag cggctgacca ctaccgagga acactgtacc cacgaccaaa
     4861 aaggtcacaa tggcgagcac tttaataagc gcaaaccaga actccatctc cgcaaaccat
     4921 ttcacaccga tcatattcat ggtgccaacg atggtaagtg cagcgagcgc aaagacccac
     4981 tgcggcacgc cgccaaacgc accccagtaa tgcatataca gagcgacggc ggtaatatca
     5041 acaatccccg tcatcgccca gttgatgaag tacatccagc cagcaacata agcggctttc
     5101 tcacccaaaa actcacgggc ataagagaca aaactgccac tggaagggcg gtgtagcacc
     5161 agctcaccca atgcacgcag aataaaaaac gaaaacaagc cacaaattaa ataaaccagt
     5221 gccagtgctg gacccgccat ttgcagtcgg gctcctgcac ctaaaaacaa gccggtgcca
     5281 atcgcgccgc caatggcgat catctgtacc tggcgattgc ccatcgcttt gtgatacccc
     5341 tcttcgtggg cattaagcca gcggcgtttc gcggcgtgtt gatctgaagt gtcggtgtcg
     5401 tgtttactca ttgctctccc tgattgcttt aatgaaaaag tcatataagt tgccatgaac
     5461 aatgtttatt catcctggcg atgatagtta tcaccattat tctttatggg tataaaacta
     5521 tcggcgcagc atcctacccg ttatcgatat acgatgcaaa acatcccctt acaatcctga
     5581 aggggattca tacaactgac gaaaaaatga caaatacttt tgctggttaa cctgtgtact
     5641 gtcctacact taatctttaa aagattgtga ggggcatatg attaaggtgt atggcgtacc
     5701 cggatggggc tctacaatca gtgagctgat gctaacactg gctgatattc cttatcagtt
     5761 cgttgatgtc agtggttttg accacgaggg aacttcacgc gagttattga aaaccctgaa
     5821 cccgttgtgt cagattccga cactggcgct ggaaaatgac gagattatga cggagacagc
     5881 ggcgattgca ttgatggttc tcgatcgtcg cccggacctt gcgccaccgg ttgggcgcgc
     5941 cgaacgtcag cagtttcaac ggctattggt ctggctggtt gctaatgtct atccaacatt
     6001 cactttcgcc gattaccctg agcgttgggc tcctgacgca cctgaacagt taaagaagaa
     6061 tgttattgaa tatcgtaaat cgctttatat ctggctgaat tcacaattga ccgctgaacc
     6121 ttatgcgttt ggtgagcaac taacgctggt ggactgttat ctttgcacta tgcgcacatg
     6181 ggggtctggg catgaatggt ttcaggataa tgccacgaat atcagtgcga ttgctgatgc
     6241 tgtatgtcag ttaccgaaat tacaagaagt attaaaaagg aatgaaatta tttgagtggg
     6301 ataccagagg attatgttat ttattat
//
LOCUS       DAJVQG010000188         6323 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJVQG010000188 DAJVQG010000000
VERSION     DAJVQG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6323
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.188"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..248)
                     /locus_tag="OQM83_003619"
     CDS             complement(<1..248)
                     /locus_tag="OQM83_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521785.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGA"
     gene            complement(250..423)
                     /locus_tag="OQM83_003620"
     CDS             complement(250..423)
                     /locus_tag="OQM83_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521786.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            524..709
                     /locus_tag="OQM83_003621"
     CDS             524..709
                     /locus_tag="OQM83_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521787.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            670..789
                     /locus_tag="OQM83_003622"
     CDS             670..789
                     /locus_tag="OQM83_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCS1521788.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1268..1534)
                     /gene="minE"
                     /locus_tag="OQM83_003623"
     CDS             complement(1268..1534)
                     /gene="minE"
                     /locus_tag="OQM83_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCS1521789.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1538..2350)
                     /gene="minD"
                     /locus_tag="OQM83_003624"
     CDS             complement(1538..2350)
                     /gene="minD"
                     /locus_tag="OQM83_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCS1521790.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2374..3069)
                     /gene="minC"
                     /locus_tag="OQM83_003625"
     CDS             complement(2374..3069)
                     /gene="minC"
                     /locus_tag="OQM83_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCS1521791.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3589..3957
                     /locus_tag="OQM83_003626"
     CDS             3589..3957
                     /locus_tag="OQM83_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCS1521792.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4065..4466)
                     /locus_tag="OQM83_003627"
     CDS             complement(4065..4466)
                     /locus_tag="OQM83_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCS1521793.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4708..5001
                     /locus_tag="OQM83_003628"
     CDS             4708..5001
                     /locus_tag="OQM83_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCS1521794.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5073..5732
                     /locus_tag="OQM83_003629"
     CDS             5073..5732
                     /locus_tag="OQM83_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCS1521795.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFISAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5824..6270
                     /locus_tag="OQM83_003630"
     CDS             5824..6270
                     /locus_tag="OQM83_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCS1521796.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
BASE COUNT         1637 a         1411 c         1501 g         1774 t
ORIGIN      
        1 gcaccaacgg cggcacccac ttttgcacct ttacgtgcat ttttaccgtc gcggcctttt
       61 tctgttactg caccaacacc agcgccaaca gctgcacctt tcagtacgcc attaacacca
      121 ttgccagtaa gtaatccaac gcctgcgcct agcaatgcac ctttcgtggt gcggttcata
      181 tccgccatcg ctggcgtgga gcagaacaat gctgagataa gcccgaaggc aagtattttt
      241 ttcttcaact tagatgtccg gtattaagta agttgcacac acaataattt cgtcttcaat
      301 taagatctgc ttaactaaag aacgctcgct attattcaga taattcaaaa tgagcgtggc
      361 tgtgatgata ggaattatgt tttttacgtg aatgagaata atcttaaatg aggaataact
      421 cattgattgg caatattttt attcaagaag tatcattgac tgttaacgca atgttgtaaa
      481 ggtaagataa tctgatttat caatattatt gtgtgatttt tatgtgagca gaagatattc
      541 atcagcaacg attacattag tcattttatt ttgccgacgg cctcattgtc gaaagataag
      601 cgtacgacag tattatctga aaagagtgat tttttatcca actacacttc agcgcactgc
      661 gtttaaaaaa tgcctctttc ttatgcggga tatcatcatt tcatcatgat gtctttggtg
      721 agcggtgaac acaatacacc tgcgctgtct cttcaggatg aatcccctgg tctggtgcct
      781 gcgggctgat gttgcagcag agcttaggtc acttagacgc tactatcatt tatccaatgg
      841 catggaatcg aaatcagtcg atacccgcag tatatatcgt gaactggtcg ttctgaccat
      901 caacgattaa actgcgcttc ggcgcagttt tcgtttacag ggggctaaaa gggaaaattc
      961 tggggcaaaa aaagcccgcc agttacggcg ggaaacctca tcctatggga gaacaatgaa
     1021 taatgaaatt gcggggttat catcccccag tgtatccaca ctaacaataa ggttatttac
     1081 tcagccaggc ataaacatta tgttctgtgc gtgggaacag ccttaaggtg taaaggggga
     1141 ggtggaaata gcaatgagga gtatcagcaa gaatactcgc cgctttacca caacgtggat
     1201 gagagggatg aaaaactcaa ggcagaaatg actctgcctt gaagataaat gcgtttttac
     1261 agcgggctta tttcagctct tctgcttccg gtaaggtcac gttcagctca agaatagaaa
     1321 tatcgccatc tttttgctca agctgtacgg ttaccatctc aggatcaatt tgtacgtatt
     1381 tacaaatgac ctcaagaata tctttacgca actgcggcag ataatgcggt tctgcatcgc
     1441 tgcgacggcg ttcagcaaca ataatctgca gccgttcttt tgcaatgttg gctgtgtttt
     1501 tcttccgcga gagaaagaaa tcgagtaatg ccataactta tcctccgaac aagcgtttga
     1561 ggaagccttt cttctcttct tcaatgaagc ggaaaggacg ttcttctccc aacagacgtt
     1621 ctacggtatc tgcgtaggct ttacccgcat cggcgttaat gtcgagaatg accggttcac
     1681 cctggttaga ggcgcgtaat actgattgat cctctgggat cacgccgacg agtttgatgc
     1741 gcaggatctc cagcacatct tccatgctca gcatgtcacc tctgcttacg cggcctgggt
     1801 tatagcgcgt taacagcagg tgctctttaa taggctcttc gccattttct gcgcggcgtg
     1861 atttcgacgc cagaatgcct aaaatacggt cagagtcgcg tactgaggag acttccgggt
     1921 tggtggtaat aatggcttcg tctgcaaaat agagtgccat taacgcaccg gtttcaatcc
     1981 ctgccgggga gtcacaaacg ataaattcaa aatccatcgc tttcagatca tcaagaactt
     2041 tggcgacccc ttcacgggtg agggcatctt tatcgcgtgt ttgcgatgcc ggaagaatat
     2101 agagattttc agtacgctta tctttaatta acgcctgatt tagcgttgca tcgccctgaa
     2161 tgacgttgac gaaatcgtaa acgacccggc gttcgcaacc cataatcagg tcgagattac
     2221 gcaggccgat atcaaaatct atcacgacag ttttctttcc cttctgggcc aaaccagtgg
     2281 cgatggccgc gctggaggtt gtcttaccaa cccccccttt gcccgaagta acaacaataa
     2341 tgcgtgccat agaaattcct tgttaaaaag ggatcaattt aacggttgaa cggtcaaagc
     2401 gttttcgact aactgcagtc gcgccgcttt gccataaaat tctgctggga tttgatcact
     2461 cagccagtat tcacctgcga tggacaccaa ttccgccatc aggttcgtac aaaatatttg
     2521 cgtttcccgg tcaccacttg cccctgccag cgcacgaccg cgcatcatgc catagacatg
     2581 aatgttccca tcggcaatca attcggcccc agcgctaacg tggcttgtaa caatcagatc
     2641 acattgtgga gcataaatac gctgaccgga acgcaccggg gtatctatta aacgcgtttt
     2701 tgtgaccggc gtggtatttt gcgctggagc ctgtggtgcg ggagctggac gtggcgcttt
     2761 ttcctttcct tccgtcagga taggcagccc cattttttca atttcggctt taagttgcgc
     2821 gtctttgcag ccacttacgc caataacccg caaaccggtt gccgaaaccg ccttatgcat
     2881 cgctgaccag tttaccgggt cttccagtgc actgacgttg agtacaacgg gggcatgttt
     2941 taaaaatgcg ggggcctgag cgattttgtc ttccagcgcc tgatggataa ccttaggttc
     3001 tgcctcatgc agatgaacca cagataaagt gaagctactg cctttaagct cgattggcgt
     3061 gtttgacatc ctggccttac tcaattagct attaatcatc gccagcgcgc gatgatgttc
     3121 cgaagactat aaggcatgtt atagtctgga ttatattgag gcaagtcacc ctcccattta
     3181 ttcagagtaa aagtctattc tgtgataaat ggcgctgatt catagcttaa aaaataccct
     3241 tgtcaatcaa cccattgccg ccgtactttt gattcttctt atttacgctt cctttttccg
     3301 caccctaatc gtgacagcat ctccagacct gtttaaatat ccagcattga ggcaagtgta
     3361 ttttttcacc acgccaggct ttcttaactt tccatttttt gcagcatcat taaagaacag
     3421 atacccgtta tcttaatatc cgccacagaa ttgctctatc atagaaaccg cagaagacgt
     3481 ctgatacttc tgtcaggtca tcgtaactcg tccaggtcag cggttttgat tacggattga
     3541 tgacgaaatg agcataagtg ttgtacggtt ggattatcag gatgtgctat gaatatgatg
     3601 cgtattattt atatcggatt gtcaggtgtg gggatgatgt tctcatcaat ggcgtctgga
     3661 catgacgctg gcggacttca atctccggcg tgcggcgttg tttgcgaccc gtatatttgt
     3721 gtgaactcag atggcatttc cccagagtta acaaggaaat atctcggcga aaaagccgct
     3781 gaaaacttac aatcattaca aggctacgat cccagcgaat ttacattcgc taacggtgta
     3841 ttttgcgatg ttaaagaaaa attatgtcgt gatgatcgtt attttggtgt agatggtaag
     3901 cggagtggaa aaatcaatca aaccaccaca aagatgttat ttatgtgtcg tgagtgatgc
     3961 cttgtttaca ttatgttctc tgctattcgt cattaacctc ttgagcgaca gagaggagtg
     4021 gggcgttaac gtctatgatg gggatagtcc ccgtcggttg acatttactt aatttgaata
     4081 tcgacgttat attttttagt tttgttttta cgggcgtcgt ttcgagtttg cagcactcgc
     4141 agctcatatt tgccagaagc agggagtgag tattgtccgt gactatcgag ttctggggaa
     4201 tatctggata aatcaacaga atcatcgatt cctggcccga agagataagt atcagcgcct
     4261 tcattagaga tactcacatg cactttctga ccttttttgg catagaaggt atatgtatcg
     4321 taatcgtatc cctttatttc gccggagtac tgagcgctgc tatggccttt gctgaactca
     4381 acattgacat ttttacccgc agcaaagctc gtggaggtta gcagtgcaag cagcaataca
     4441 gccttactga tgctcttgat tttcattggt agtctcctgt tcagaatgag tacgatgaac
     4501 tgttataata taacaatccc ttacgggaga attccagtgg cttttctgcg ttcggtaaaa
     4561 tcccggcgcg gcattttttg ccggggggat tacttataaa ggggcatgga ctcaacaaac
     4621 cgcagaaatc tacagctggt tggcttgtgg cgcgggtttc attgatgtta gcttatgcca
     4681 aaacctggca tcttaaagag taaaagtatg ttttgtgtga tttatcgaag cagcaagcgt
     4741 gaccagacct atttatatgt cgaaaaaaaa gacgattttt cgcgtgttcc tgaggaactg
     4801 atgaaaggtt ttggtcagcc tcagttagcg atgattctgc cgttggatgg gcggaagaaa
     4861 ctggtcaatg ctgatattga aaaggttaaa ctggcattaa ccgagcaagg ttactatttg
     4921 cagttaccgc caccacctga agatttgctg aagcaacatc tttccgtcat ggggcagaaa
     4981 acagacgaca ctaacaaata accgatatcc ggcggtggca ttatctttgt cggcgcgggt
     5041 tttcatatcc acgataaggt gaggggaacg ttatgtatca acatcacaac tggcaaggtg
     5101 cgctgctgga ttatccggtg agtaaagtag tctgtgttgg cagtaactat gccaaacata
     5161 ttaaagagat gggcagcgca gtgcccgaag agccagtgct gtttattaaa ccagaaacgg
     5221 cactgtgcga tctgcggcag ccattggcga tcccatccga tttcggttca gttcatcatg
     5281 aagtcgaact ggcggtgttg attggcgcga cgctgcgtca ggcaacggaa gagcatgtcc
     5341 gcaaagccat tgccggttac ggcgtggcgc ttgatctgac attgcgtgat gttcagggaa
     5401 aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc gtttgataac tcttgtccgc
     5461 tttccgggtt tatctccgcg gcggaattta ccggcgatcc acaaaataca acgctgggcc
     5521 tgagcgtaaa cggcgaacaa cgccagcaag gtacgactgc ggacatgatc cataaaatcg
     5581 ttccgttgat cgcttatatg agcaagtttt ttaccctcaa ggccggtgac gttgtgttga
     5641 caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga tgagctgaca gtcactttcg
     5701 atgggcattc tttgacaact cgcgttttgt aatacttttt gccgcctgaa agcggcggca
     5761 aaacttgcat cgctgtgcca gactggttat aaggtgcgtt ttaacgtaat ggcggaacac
     5821 ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg acgaaatgag cgatgcggaa
     5881 tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcctgc ataaactgat ggatgaagac
     5941 accgacgaaa tctacttcac taacgtcgcc tgtcgccagc tcaatattaa aacttgtcaa
     6001 tgtcggaact acgaacgtcg tttcgagttt gaacccgact gcattaaatt aacccgtgaa
     6061 aatctgccaa cattcgaatg gctgccaatg atctgcgctt atcgtttgct ggcggaaggt
     6121 aaagatttac ctgcgtggca tccgctactt actggttcga aagcggcaat gcatggtgaa
     6181 cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag tgattgactg gcaggatcat
     6241 atcttaaata aacctgactg ggcgcagtga taaatttaat agatgttgat gggggctttc
     6301 ttaatatacg tctggatgct cgc
//
LOCUS       DAJVQG010000189         6319 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJVQG010000189 DAJVQG010000000
VERSION     DAJVQG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.189"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..438)
                     /locus_tag="OQM83_003631"
     CDS             complement(<1..438)
                     /locus_tag="OQM83_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCS1521797.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQA"
     gene            complement(579..2612)
                     /gene="metG"
                     /locus_tag="OQM83_003632"
     CDS             complement(579..2612)
                     /gene="metG"
                     /locus_tag="OQM83_003632"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCS1521798.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2744..3853
                     /gene="apbC"
                     /locus_tag="OQM83_003633"
     CDS             2744..3853
                     /gene="apbC"
                     /locus_tag="OQM83_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCS1521799.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4098..4379
                     /locus_tag="OQM83_003634"
     CDS             4098..4379
                     /locus_tag="OQM83_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCS1521800.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4622..5164
                     /locus_tag="OQM83_003635"
     CDS             4622..5164
                     /locus_tag="OQM83_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1521801.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            5244..5918
                     /locus_tag="OQM83_003636"
     CDS             5244..5918
                     /locus_tag="OQM83_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS1521802.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            5934..>6319
                     /locus_tag="OQM83_003637"
     CDS             5934..>6319
                     /locus_tag="OQM83_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1521803.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSV"
BASE COUNT         1677 a         1433 c         1516 g         1693 t
ORIGIN      
        1 tgcttgctga acaggaactc cgggaaagcg gcgatgactt aacgtggtgt gtacaacttc
       61 gggcggaaaa acgacagcaa tatctttcgc cagccacggt aaaacagcat ctgtattttt
      121 gtcaggcgca cgggtttctg ctacgaaaga gggttgttcc tgaataatgg tggataagtc
      181 cgctaccttg agagagcccg ttaccgtttg ggaaggttgc aaagaattat ccttcgcttg
      241 ctccacatac cccttcttca ccttctccgc aatcagctta agctccgctt ttgccgctgc
      301 cgcagcatct gaaaaacttt ttatctggct ttgccctttg gtgccaactt ttccccaact
      361 gatatgcaac tcgtttccct gttgctcaac tgcccagaat ttatgtgatt tttcgtcctg
      421 atagataaag tgtctcattc tgcatccctg taattttatc cagttgaatg cagatgctac
      481 cagtatttat gcgggttaga gagagacaaa atcgcaagaa aacaacttta ggaatttata
      541 aagaaaaggc gctgtcgatg cagcgccttg aagggggatt atttcacctg atggcctggt
      601 ttagcaccag catccgggct taacaggaag atatctttcc cgccaggacc agcagccatt
      661 accatgcctt cggagatacc aaagcgcatt ttacgcggtg ccaggttggc caccataatg
      721 gtgtgacgcc cgatcagtgc ctgcggatcc gggtaggcag aacgaatacc ggagaagaca
      781 ttgcgttttt caccgccgag atccagtgtc aggcgcagca gtttgtcaga accttcaaca
      841 aactctgcgt tttcgatcag cgccacgcgc aggtcaactt ttgcgaagtc gtcaaaggtg
      901 atggtttcct gaatcggatc atctgccagt gggccagtaa ctggcgcggc agctgctttc
      961 acttcttctt tagacgcttc caccagtgct tcaacctgct tcatatcgat gcggttatac
     1021 agcgctttga acggattcac tttgtggccc agcagcggtt gctggatacc atcccaggtc
     1081 agttctgtat tgaggaatgc ttctgcgcgc tcggtcagtt tcggcagtac cggcttcagg
     1141 taagtcatca gcacgcggaa caggtttatg cccattgagc aaattgcctg caggtcggca
     1201 tcgcggcctt cctgttttgc caccacccac ggagcctgct cgtcgacata gcggttagcc
     1261 agatcagcca gcgccatgat ttcgcgcact gctttaccaa attcgcggct ttcccacgct
     1321 tcaccaatca cttcagcggc atcagtgaag gttttgtaca actgcgggtc agccagttcg
     1381 cttgccagca cgccgtcaaa acgcttgttg ataaagcccg cattacgaga ggccaggtta
     1441 accactttgt tcacgatatc ggcattcaca cgctgaacga aatcctccag gttgagatcg
     1501 atatcatcaa tgcgcgaaga gagtttcgca gtgtagtagt aacgcaggct gtcagcgtca
     1561 aaatgattca gccaggtgct ggctttaata aaggtgccgc gagacttgga catctttgcg
     1621 ccgttcaccg tcacatagcc atgaacaaac aggttggtcg gcttgcggaa gttgctgcct
     1681 tccagcatgg caggccagaa caggctgtgg aagtaaacaa tatctttacc gatgaagtgg
     1741 tacagctcgg cggtggagtc tttcttccag tattcatcga agcttacgct gtcgctgcgc
     1801 ttgtcgcaca gattcttgaa agaacccatg tagccaatcg gtgcgtccag ccagacgtag
     1861 aaatatttgc ccggcgcgtt cggaatttca aaaccaaagt atggcgcgtc gcgggagata
     1921 tcccactgtt gcaggccgga ttcaaaccac tcctgcattt tatttgccac ctgctcctgc
     1981 aacgcaccgc tgcgggtcca tgcctgcaac atttcgctga aagagggcag atcaaagaag
     2041 aagtgttcag aatcacgcat taccggcgta gcgccagaaa ccaccgattt cggctcgatc
     2101 aattcagtcg ggctgtaggt cgcgccgcag acttcgcagt tatcgccgta ttgatccggg
     2161 gatttacatt tcgggcaggt gcctttcaca aaacggtccg gcaggaacat gcctttttcc
     2221 ggatcgtaca gctgagagat ggtgcggttt ttaataaaac cgttctcttt caggcgagag
     2281 tagataagtt ctgacaactg gcggttctct tcgctgtgcg tcgagtgata gttgtcatag
     2341 ctgatgttaa agcctgcgaa atcagtctga tgctcctgac tcatttcgcc aatcatctgc
     2401 tccggggtga taccaagctg ctgagctttc agcatgatcg gtgtaccgtg ggcatcgtcg
     2461 gcgcagatga agttgacctc gtggccgcgc attcgctggt aacggaccca gacatcagcc
     2521 tggatgtgct ccagcatatg gccgaggtgg attgagccgt tagcgtacgg cagtgcgcac
     2581 gtcaccagaa ttttcttcgc gacttgagtc atagtaggca ttacttctta atagtgaaaa
     2641 ggggttagat agtaccaaat gggaaaatgt taagtaagta cttctctata ttcttcaggt
     2701 aaactatcag ctgatggttt atctatacaa aaaaggagtc gggatgaacg aacaatccca
     2761 ggccaaatca ccagaagcct tgcgcgcaat ggtcgccggg acactggcca attttcagca
     2821 cccaaccctg aagcataacc tcaccacgct gaacgcgttg caccatgtcg cctggatgga
     2881 cgacacgttg catgtggaac tggttatgcc attcgtctgg catagcgcgt ttgaagagtt
     2941 aaaagagcaa tgtagcgccg aactgctgcg tatcaccggg gcgaaagcta tcgactggaa
     3001 actgtcgcac aatatcgcca ccttaaaacg cgtgaagaat cagccgggta ttaatggtgt
     3061 gaagaacatc attgcggtca gttccggtaa aggcggcgtg ggtaaatcct ccacggcggt
     3121 aaacctggcg ctggcactgg cggcggaagg ggcgaaagtc ggcattctgg acgccgatat
     3181 ctacggccca tcaattccca ccatgctggg cgcagaaaat cagcgtccaa cttcgccgga
     3241 cggtacccat atggcaccga ttatgtctca tggcctggca accaattcaa ttggttatct
     3301 ggtcactgac gacaatgcga tggtgtggcg tggaccgatg gcgagcaagg cgttaatgca
     3361 gatgttgcag gaaaccttgt ggccggatct cgactatctg gtgcttgata tgccgccagg
     3421 caccggtgac attcaattga cgctggcgca aaacattcca gtaactggtg cagtcgtggt
     3481 caccaccccg caggacatcg ctttgatcga tgcgaaaaaa ggcattgtga tgttcgagaa
     3541 agtcgaggtt ccggtgcttg gtattgtcga gaacatgagt gtgcatattt gcagtaactg
     3601 tggtcaccat gaaccgattt tcggtaccgg tggtgcagag aaactggctg aaaaatacca
     3661 cacacagctg ctggggcaga tgccgttaca catctcctta cgtgaggatc tcgataaagg
     3721 cacgccaacg gttatcagcc gtccggagag cgaatttacc gctatctacc gccagctggc
     3781 tgaccgcgtt gcagctcagc tctactggca gggtgaagtc attccaggcg agatttcctt
     3841 ccgcgcggtc taacgcctat taaccattct ggttattctt catacaccgc agagatgtta
     3901 cattgatgcg gtgtattagt ttttgccgca ttaatgaaca cttatttaat actgcgaata
     3961 ttctgcggac cttcgtccga aactctacaa agtcgattga tcatacaggc aatgcttcat
     4021 tatcatcctt cgtggctgtg tagtggatgt acagctatag tttgttatgc actcattcta
     4081 tccaggcata accttcaatg aataagtatt ggctgtcagg aattattttt cttgcctatg
     4141 gattggcttc tcccgcattc tcttcagaga ctgccacatt agcgattaat ggcaggatca
     4201 gtcccccaac atgcagtatg gctatggtta atggtcaacc tcaaccgcat tgtggtcagt
     4261 taacgtataa tgttgacacc cgtcatctgt tttcttcgcc cgtcaaaggt gtaacgacag
     4321 aagtggttgt tgcaggtagc gatagcaaac gtcgaatcgt gttaaatcgc tacgattaat
     4381 ttggcaacct gatttttatt gatatcaaca ataccattta cataaatcta cattaattga
     4441 tagatgcgat aaatagttaa tactcttaaa tatttgttgt atttatattg caatggttaa
     4501 tttgtttcaa ataaataaaa caatgtgttt acattgcggt aacttttttg atgtgagaaa
     4561 tttgtttcca agagatacag actcttaaca accaaaaaaa aattttatta aggaacatat
     4621 tatgaaacgt tcaattattg ctgccgctgt cttttcttct ttttttatga gcgctggagt
     4681 atttgctgca gacgttgata ccggaacatt aactattaag gggaatattg cagaatctcc
     4741 gtgtaaattc gaagcgggtg gtgattcagt aagtattaat atgccgactg taccaaccac
     4801 tgtctttgaa ggtaaagcta aatattctac ctatgatgat gcagtcggtg taaccagcag
     4861 catgttaaaa attagctgcc cgaaagaagt tgctggtgta aaactctcgt tgattaccaa
     4921 cgataaaata accggtaacg ataaggcgat agccagtagc aacgataccg tgggttacta
     4981 tctctattta ggtgataaca gcgatgtcct ggatgtttct gcacctttta acattgagag
     5041 ttataaaaca gcggaaggtc aatatgctat tccgtttaaa gcaaaatacc tgaaactgac
     5101 agataactca gtgcaatcag gtgatgtgtt atcttctctg gttatgcgtg tggcgcagga
     5161 ttaataaata aaattgtaag tttctccagg taataaacat ggccgctatc ccatggcggc
     5221 cttttaattt aagaggcatt aaaatgaaag gattattatc tttactcatt ttttctatgg
     5281 tccttcctgc acatgccgga attgttatct acgggacgcg cattatttac ccggcagaaa
     5341 ataaagaagt gatggtgcag ttgatgaacc agggaaaccg ttcttcgctg ctgcaggcgt
     5401 ggattgatga tggcgatacg tcattaccac cagaaaaaat tcaggttcct ttcatgttaa
     5461 cgccaccagt ggcaaaaata ggggcaaatt ccgggcagca agtaaaaatc aaaattatgc
     5521 cgaataaact gcccactaat aaagaaagca ttttttatct gaatgttctg gatattccac
     5581 caaatagtcc agagcaagaa ggtaagaatg cactgaagtt tgcgatgcaa aacagaatta
     5641 agttgtttta ccgaccagcg ggtattgctc cggtaaataa agcgacattt aaaaaattgc
     5701 tggtaaatcg cagtggcaat ggtttggtga taaaaaatga ctcagctaat tgggtgacga
     5761 tttcggatgt caaagctaat aatgtcaaag tcaattatga aactattatg attgccccct
     5821 tagaaagtca gagtgttaat gtcaaaagta ataatgcaaa taactggcat ctgaccatta
     5881 tcgatgacca tggcaactat attagtgaca aaatttaatt gcaggagctg cctatgttga
     5941 gaatgacccc acttgcatca gcaatcgtag cgttattgct cggcattgaa gcttatgcag
     6001 ctgaagaaac ctttgatacc cattttatga taggtggaat gaaagaccag caggttgcaa
     6061 atattcgtct tgatgataat caacccttac cggggcagta tgacatcgat atttatgtca
     6121 ataagcaatg gcgcgggaaa tatgagatta ttgttaaaga caacccgcaa gaaacatgtt
     6181 tatcaagaga agttatcaag cggttaggca ttaatagcga taacttcgcc agcggtaagc
     6241 aatgtttaac atttgagcaa cttgttcagg gtgggagcta tacctgggat atcggggttt
     6301 ttcgtctcga tttcagtgt
//
LOCUS       DAJVQG010000190         6156 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJVQG010000190 DAJVQG010000000
VERSION     DAJVQG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.190"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..523)
                     /locus_tag="OQM83_003638"
     CDS             complement(<1..523)
                     /locus_tag="OQM83_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCS1521804.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNR"
     gene            complement(578..1325)
                     /gene="traX"
                     /locus_tag="OQM83_003639"
                     /pseudo
     CDS             complement(578..1325)
                     /gene="traX"
                     /locus_tag="OQM83_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            complement(1345..2343)
                     /locus_tag="OQM83_003640"
                     /pseudo
     CDS             complement(1345..2343)
                     /locus_tag="OQM83_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(2338..2649)
                     /locus_tag="OQM83_003641"
                     /pseudo
     CDS             complement(2338..2649)
                     /locus_tag="OQM83_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(2646..3032)
                     /gene="trbI"
                     /locus_tag="OQM83_003642"
     CDS             complement(2646..3032)
                     /gene="trbI"
                     /locus_tag="OQM83_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HCS1521805.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PMTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(3029..4279)
                     /locus_tag="OQM83_003643"
                     /pseudo
     CDS             complement(3029..4279)
                     /locus_tag="OQM83_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            complement(4281..4463)
                     /locus_tag="OQM83_003644"
                     /pseudo
     CDS             complement(4281..4463)
                     /locus_tag="OQM83_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4485..4796)
                     /gene="traL"
                     /locus_tag="OQM83_003645"
     CDS             complement(4485..4796)
                     /gene="traL"
                     /locus_tag="OQM83_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HCS1521806.1"
                     /translation="MLGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(4811..5161)
                     /gene="traA"
                     /locus_tag="OQM83_003646"
                     /pseudo
     CDS             complement(4811..5161)
                     /gene="traA"
                     /locus_tag="OQM83_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            complement(5207..6156)
                     /locus_tag="OQM83_003647"
                     /pseudo
     CDS             complement(5207..6156)
                     /locus_tag="OQM83_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    complement(6023..6139)
                     /locus_tag="OQM83_003647"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         1316 a         1889 c         1533 g         1418 t
ORIGIN      
        1 tccggttctg gcggcggatt ttatccagac gcgctgccgc atacgcttct tcctcctgag
       61 aaatatgctc cgtcacatac ccttccgtgt cataccggca ggcaccggct ttcatggcac
      121 acagatagct ttctgaacgg gtgatggcct tcagcgccct gcgcagtttt ttatgcgaga
      181 gcgggatatt ccgctgcgcc acgtcttcca gtaacacgtc ccggataccg caggccagaa
      241 gccggggggt gtcaccgtca aataatcccg gccaccaggg tttcagggta ttcacggcct
      301 catccagcgt gggcaggttc agataaatgg acagtgcctg tctggcctgc gctttttttg
      361 ccgccagctc tgcttcccgg gctgctttct cggccagctt ctgctttttc accttccact
      421 ttggtggcgt ggtgacattg atgatggttt tccggctgcg gacaggcgtc tccccttccg
      481 ttttccgctt cagtgtcagt accggtcgct tctgctctgt catgctgtgt tctgcctctg
      541 acgaatcatc ctgttacggt caaagatacc acacccgtca cagcgccaga acgcccagca
      601 cagccagatg acaggcgtag aataccggga aaaaatcccc gggccagaaa cggggtaatg
      661 attttcctgc acacgacacc agaccaacgg tcagcaccgt catcacaaga ccggccaccg
      721 ccgctgcatc actggtggca aggttaagcg ccggaataac agccagcagg caggcgacca
      781 gcgccagacg ttccgctctg tcttccgccc ggtacagtcg gtggctgact gccagcatca
      841 gcagcccggc aatgccgtaa ctggtgccgg acaaaggccc ccaacagggc catcagaaga
      901 atggccgctg ctgtacgcca cccactgcgc gtttcacacc acgtcagcac ctgtgccgcc
      961 actgcaaagg caaacaggat attcccctca taccagggaa agcctgccag ataatatgcg
     1021 aactgggcaa taattcccca tccccacagc cggttaatgg ccggttgccg gatatgcgca
     1081 tgacgggaca gattcagccc ccacaccagg gcaaaaagcg gaaaggctcc tcgcccggcc
     1141 agaaacatcc attcctgctt cagctggaat atcagattga tgtggtccag taccatcagc
     1201 accagtgcca ccgttttgat gatatcccgc tgtccgggtg accagaccag aaatgactgc
     1261 aaccaggtat cggtccgtgc agccagtgaa tcgttacgtg tcgtgttcgt gttatccgtt
     1321 gtcatcagtg tttgtcttcc tgtatcagtc tccgcccggg gttttctctt tctgcaggtc
     1381 acgaaccata tcccgctcca tctgctgcag acgctcctgc agcagatttt cccgggcaac
     1441 ctcccggacc gcttcccgct cccgttgtga ttcacgcagc acactctccg gcagcgcggc
     1501 ttcccgttca gttatggcag cccggtcacg ctcctgcccg gcaatttccc tgacagccag
     1561 ctctgttttg ccgtccggca ggtcaggttt gttctctgcc attttacgga caatttcatc
     1621 tgcgcgacgt tccgtttcac ggcggatggc ctcttcagcc tgccgctgtg ccagtacctc
     1681 tgccgtgacc ggttcgccat ttccggctcc cggctggacg ctgttgtccg ggatatcccc
     1741 ccacacgcgg ccatcggtga tggcaccggg attccacgga cggccttcac cggcaatgct
     1801 taccaccaca ccactgtcag gattatcacg ggcaatccgg acgccatcct gcatattatc
     1861 agccagcagg ctttctccgt tacggctgcc ctgcagggcc acgaactgcg catccccgct
     1921 gccttttacc cgaccttcac cgctgaatcc ccgcagcccg tttccgtcat ccgtggtcag
     1981 cgggttcagc cagataccgg cggacttgcc gttacggtca aacgccggca gtgccacata
     2041 cggttgcgga tatttacgtc ccggagcaat aaaccgtgca ggactgtctc ccccggccag
     2101 ccccgcctga cggagaacgg cacggcctgc cgccacgtcc cgcagctccc gcgccgtact
     2161 gaacagccgc tctgcattca tgacctcccg gtccggtttc ggctcaaaca catcgtgggc
     2221 agttcctttc tgtacggcat tgttgatggc atccgtccag ccctgacggt tatcggtgta
     2281 cacctgcaca tgctgcttca tacgcgacag ggccacgtag gctgactcaa agccggccac
     2341 ccgcccttcg ttatcccgga tatccgcagc cagtctgacg gacgggtcaa acaaccggct
     2401 gctgtatttc tccgtgcgtc cgataccggg cacggcaggg ggccgcagac tgttgcggat
     2461 cacccgctcc ttaaaggcat ccattttccg gcccatctca ccggactgct ccagtgccgt
     2521 caggcgctgc atgatcaccg tcagcatgtc cggctccttt accggccaca gatcacccca
     2581 ggtgccaaga tcggcggcgg ccacactctg cccccatatc agcagggcaa tcagcccccg
     2641 gcatctcatg gttccgctct cattcgccgg gcaatatcct gctggatttc ccgggtgata
     2701 tccggagccc cctgtaccac ggcaggcgac accagaatga ccgcgtggtg tttctgctgc
     2761 cacgcctgca ggctggcttc cagtgccgtg ttaaaacgcg ctgaaagggc ttttgactgt
     2821 gcttctgaca gttgtttctg actggcgctg tcaaaaaatg catccacagt ctgtttcatg
     2881 ttgaaggcgg cagtcatcgg tgcgttcagt ctgacaatgc cgtaactgac ggctgcgttc
     2941 agtaacacca ttgccaggca tcccggcacc gtccaccacc agtggctgcg tcggggggct
     3001 gtaacatcag tcgttttttg cgttgtggtc atgcaacact cctgtatttc tcatgttctt
     3061 ccagccaggc ttccagcgaa gccatctccg gccctgactt cttccacgcc agctgccaca
     3121 ccgcctcatg aatactcagc ccctctttac gtttctgctg cacaaactca aaatccgggc
     3181 cgtcagagct gtacatggcg cagcttaacg ggtccacaaa caggcggtgc caggaggaat
     3241 ggttttccac ctgcagcagg aaggaactga accactggtc tttggccgca ccaaacttac
     3301 caatcatgtc gcgctgcagt ggctggaact ggtccggata cagctggttg tattttgcga
     3361 actccttcgc actctgtttg aggataattt tgtaggagga gttaccccat gccgcacggg
     3421 ccgcactgga ggccttgtca gagtcaaagt cgacgatgtt ctgtgtgatg gtgatataag
     3481 caccggtatg acgacgggcg gtacggtaac ctttctcaat aaactcaccg accttgtgat
     3541 ttttgaagtc cagcagacgc cagccttcat caatgacgtt cagtttcttg aggttacgcg
     3601 gcgtgcggta catcctgttc tcgatgtaga taatcaggga gaacatcacc gcaaccagca
     3661 gtgacggacg gtcttccagt ccgcccagct ccagcaccac catttttgcg tcatcccgca
     3721 gggacggctc atcagagtta aaatactggc cgtaagtgcc gttggcagtg tactggtcaa
     3781 gcagcacaat catttcgtcc agacggctgc ggatagtcgg tgacccggca tactgctcgc
     3841 tgtcactggc gtttttcagg aaatccacca cgtcatcaat acgtgccctg ttctctttgg
     3901 ccagccagga ggccctgacc gcctgcagca gcagaccttc atgcacttca tccaggttac
     3961 cgttggggct ggccatcact gacaactggt cacggacacg ctccgctgac tggtcgatat
     4021 cggtgatgtt cgcaaacggg ttaaaacgca gggtttcacc gtccagatat acgccaccca
     4081 tgttctcaca cagggacttg tagccatccc ccatgtcgaa caccacggca aagcccccgg
     4141 agtccagcac gctgcggata agtggctgta tcagcccggt tttaccggcc ccggaggtgc
     4201 cacagaccgc catgttgtag ttggtgttat tcattccccg gaagaaaata tcgataaacg
     4261 ccagctggtt acggtaggtg tgacacggca aagggcacat taaatcccat tggggtcacg
     4321 gcagtgcgtt gctcattctg aagacggtag ttattcaccc cctgaatgag gttaacagag
     4381 aggctgagaa caataagcac tgacataaat ataaaggcga tggccattac acgactggta
     4441 cttaaacggg caccgtgttc catataacaa tcctggtatc agttctattt aatccactgc
     4501 cggaaacacg aatcgggaac attatgaaaa ataccgcgca gcagggctgt tggcatatac
     4561 cagtaaatca ggtcacgtaa ccaggaactg ccccgccctt ttttcagttt tttaatcccg
     4621 aaataaacca gaaccgctgc accaataccg aacagatatt tcgatgttgt gataccccag
     4681 ccaacacaga ttgctgcggg gatcagttca tccagtggca ggccaaacca gcggctctgg
     4741 ttggtcagtg tttccgggaa acgatatttc ttaagtttat tctcgtctcc caacatcgtt
     4801 ttatttcctg tcagaatccg gcaacagaca tacctactgc aataaataca gagagtatgg
     4861 caaagccgac caggaacttg acgtttttgg tcatcatgta catgactgca ccgaccagaa
     4921 cttcagccag aacaacccat ttaacgatac tggagtcctt accgaaggtc gccttaaccg
     4981 tttcctgacc tttagccatc aggtccgtgc ctttaccagc agccatagct aactcaggga
     5041 acaacatcat cagaacagca accgggatca ctgcacgaac caggcgaagc atattcagac
     5101 gagtgaattt agaaaaaaac gatttctttt tgacgggcgc agaagtaccc tgaacactta
     5161 acgctgaacc gccccgggtt tcctggagag tgttttatct gtgaactcag gctgccagat
     5221 catcgtttcc gatggaagca taataagctt tttctgcttc tgccggagga gtatggccca
     5281 gcctttccag caatcgtcga ttgttatacc agtccaccca cgtgagtgtg gccagctcca
     5341 cttctgtccg gtttttccag ctcttacggt gtattacctc cgctttgtaa aggcccttga
     5401 tgatctctgc catcgcgttg tcatacgagt cacctgtact tcctgttgat gccagtaatc
     5461 cggcttcctt aagccgctgt gtgtaggcca gcgatacata ctgagaacct ttatcactgt
     5521 gatggaccgt gccggacggt cgacgggccc ataacgcctg ctccagtgca tccagcacga
     5581 atgtcgtttc catggacgat gagacccgcc accccacgat gtatccggca aacacatcaa
     5641 tgatgaacgc cacatagacg aagccctgcc atgtgctgac gtaagtaaaa tcagccaccc
     5701 acagctggtc aggtcgttct gccacgaact gacggtttac gcggtcgcct gcggcaacgg
     5761 ctttccggct gatggtcgta cggacctttt taccccggag aacaccggca agtcccataa
     5821 ccgccatgag acgtgccaca gtgcatctgg ccactctgat accttcccgt aacaactgac
     5881 gccagacttt acgcacaccg tataccttgt gattttcatc gtatacgcgc tgtatctctt
     5941 tcttcagcca gtcatcgcgc tgcgcacggg cactgcgttt atccggatga tgtcgctgtt
     6001 gctgacagtg gtaatacgtt gacggggcaa tatgcagttc gctgcatagc ggtccgaccc
     6061 cgtactgctc acgcagctta tccagcagtg gcatcatttt ttccagaggc ggtcaaactc
     6121 cgccttcgca aaataagcgg aagcctggcg aaggat
//
LOCUS       DAJVQG010000191         6147 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJVQG010000191 DAJVQG010000000
VERSION     DAJVQG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.191"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            163..317
                     /locus_tag="OQM83_003648"
                     /pseudo
     CDS             163..317
                     /locus_tag="OQM83_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            363..779
                     /gene="hicB"
                     /locus_tag="OQM83_003649"
     CDS             363..779
                     /gene="hicB"
                     /locus_tag="OQM83_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HCS1521807.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            858..2264
                     /locus_tag="OQM83_003650"
     CDS             858..2264
                     /locus_tag="OQM83_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCS1521808.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRE
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            2509..3654
                     /locus_tag="OQM83_003651"
     CDS             2509..3654
                     /locus_tag="OQM83_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1521809.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            3672..4685
                     /locus_tag="OQM83_003652"
     CDS             3672..4685
                     /locus_tag="OQM83_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1521810.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            4686..5586
                     /locus_tag="OQM83_003653"
                     /pseudo
     CDS             4686..5586
                     /locus_tag="OQM83_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            5576..>6147
                     /locus_tag="OQM83_003654"
     CDS             5576..>6147
                     /locus_tag="OQM83_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCS1521811.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALL"
BASE COUNT         1418 a         1499 c         1649 g         1581 t
ORIGIN      
        1 ttcagttttg caaattagcg caaagaaatt ctggaatctt ccttcctgat tttgcattgc
       61 attctgccgt tgcggcgatt tagtgctatt ttcgagcaaa ttacacatag gggtaaacac
      121 taatgtttac tttattgata tactcagcgg cagggaggcg atgtgaaaca aagcgagttc
      181 agacgttggc tcgaatctca gggcgtcgat gtagcgaatg gccgcaacca tttgaaactc
      241 aggtttcatg ggaggcgcag tgtcatgccg cgtcactcct gcgtaaagca atcctgaaac
      301 aactcggttt gagttaatcg ccaattaaaa aggttaatga catgcgagag acagtcgaaa
      361 ttatgcgtta tcccgtcact cttacacccg cgccggaagg cggttatatg gtttcttttg
      421 tggatatccc tgaagcgttg acgcagggcg aaactgtcgc tgaagcgatg gaagcggcaa
      481 aagatgcttt actgaccgca tttgattttt attttgaaga taacgagctt atcccgttac
      541 cttcgccatt aaatagtcat gatcacttta ttgaagtacc tttgagcgtc gcctctaaag
      601 tattgctgtt aaatgctttt ttacagtcag aaatcactca gcaagagtta gccaggcgaa
      661 ttggcaaacc taaacaggag attactcgcc tatttaactt gcaccatgcg acaaaaatcg
      721 acgccgtcca gcttgcggca aaggcgcttg gcaaagagtt atcgctggtg atggtttaat
      781 tacagttaac gaaaagttgt catttttaac aactgatata gactgccgaa tcatctgcac
      841 ataattacga ttcgataatg aaaaaatacc agcagcttgc agaacaatta cgcgagcaga
      901 ttgcgtcggg tatctggcaa cccggcgatc gtttgccttc gttgcgtgac caggtggcgc
      961 tttcaggcat gagctttatg actgtcagcc atgcctatca gttgctcgaa agtcagggat
     1021 atattatcgc acgaccgcag tcgggttatt acgttgcgcc acaggcaata aaaatgccga
     1081 aagcgccagt cattccagtc actcgagatg aagcagtcga tatcaacact tatatttttg
     1141 atatgttgca ggccagtcgc gaaccgtcgg tcgttccgtt tgcctcggcc tttcccgacc
     1201 cgcgactttt ccccctccaa caactaaacc gctcgctggc gcaggtaagc aaaaccgcca
     1261 cagcgatgag cgtgattgaa aacttaccgc caggaaacgc agaactgcgt caggctattg
     1321 ctcgtcgcta tgccttacag ggcatcacca tttctcctga tgaaattgtc attactgccg
     1381 gggcgttaga ggcattaaac ctcagtttgc aagcggtaac tgaaccgggc gattgggtga
     1441 tagtagagaa tccttgtttc tacggtgcgt tgcaggcgct ggagcggcta cggctgaagg
     1501 cgttatcggt ggcgacggat gttaaagaag ggatcgatct tcaggcgctg gaactggcgt
     1561 tgcaggatta tccggtgaaa gcgtgctggc tgatgactaa tagccagaac ccactcggat
     1621 ttaccttaac gccgcaaaaa aaagcacaac tggtggcgtt actcaatcag tacaacgtaa
     1681 cgctgattga agatgacgtt tacagcgaac tttattttgg acgggaaaaa ccgctgcctg
     1741 cgaaagcgtg ggatcgccac gatggcgttt tgcattgctc ttcgttttcg aaatgtctgg
     1801 tgcctggttt tcgtattggt tgggtcgccg ccggaaaaca tgcacgtaaa attcaacgct
     1861 tgcagttgat gagtacgctt tccaccagct caccgatgca acttgcgctg gtggattacc
     1921 tttccacgcg ccgatacgac gcccatcttc gtcgcctgcg tcgccagctt gcggaacgta
     1981 aacaacgtgc ctggcaggca ctgctgcgtt atctgcctgc ggaagttaaa attcatcata
     2041 gtgacagcgg ttattttctc tggctggagc tccccgagcc gttagatgcc ggcgaattaa
     2101 gcctggcggc actgacgcat catatcagta ttgcgccggg taaaatgttt tctaccggtg
     2161 aaaactggtc acgttttttt cgttttaata ccgcgtggca gtggggagaa cgtgaagaac
     2221 aggcggtaaa acaattaggc aaacttattc aagaacggct gtaatagcgt ttaatttaat
     2281 tcctcttaga ttgggtaata tgaatttcga atagcagtca tatttcctaa ccccttgtct
     2341 atactccaga agataacctt acagacggca taatgcgcgg tagctcacaa cctgattaaa
     2401 ttttctcagg gacgaaggtg tgcctgcaag ccgccgtcta tggttaaaca aggagatatt
     2461 tttacggcac ggcggctgaa caattaatta cgacaggagt aagaccttat gagcaagaca
     2521 tttgcccgca gcagcctgtg tgcgctcacc atgacaataa tgaccgctca cgccgccgaa
     2581 ccacctacca atttagataa accggaaggg cgactggata ttatcgcctg gccgggatac
     2641 atcgaacgcg gacaaactga taaacaatac gactgggtaa cgcaatttga aaaagagaca
     2701 ggctgcgcgg tgaatgtgaa aaccgccgcg acttccgatg aaatggtcag tctgatgacc
     2761 aaagggggtt acgatctggt tacggcatcc ggcgatgcct cgctgcgttt gattatgggc
     2821 aaacgcgtgc agccgattaa taccgccttg attcccaact ggaaaacgct cgatccgcgc
     2881 gtggttaaag gcgactggtt taatgttggc ggcaaagttt acggcacacc ttaccaatgg
     2941 gggccgaacc tgctgatgta caacactaaa accttcccga cgccgccgga tagctggcaa
     3001 gtggtttttg ttgagcaaaa tctgccggac ggcaagagca ataaaggccg cgttcaggct
     3061 tatgatggcc ctatctacat tgcggacgct gcgttgttcg ttaaagccac tcagccgcag
     3121 ttgggcatca gcgatccgta tcaactaacc gaagaacagt accaggcggt gctgaaagtg
     3181 ctgcgcgctc aacacagttt gatccatcgc tactggcatg acactaccgt gcaaatgagc
     3241 gatttcaaaa acgagggtgt ggttgcttcc agtgcctggc cctatcaggc caacgccctg
     3301 aaagccgaag gccagcctgt tgctaccgtt ttcccgaagg agggtgttac cggttgggct
     3361 gataccacca tgctgcatag cgaagcgaaa catccggttt gcgcctacaa atggatgaac
     3421 tggtcattaa cgccaaaagt gcagggcgat gtggcggcct ggtttggctc gttaccggta
     3481 gtgccggaag ggtgtaaagc cagtccgtta ttaggcgaaa aaggttgtga aaccaacggt
     3541 tttaactatt tcgacaaaat cgccttctgg aaaacgccta tagcagaagg gggcaagttt
     3601 gttccctaca gtcgctggac gcaggattac attgccatta tgggcggtcg ctaacttcgc
     3661 tggggtgctt tatgacgtac gcagtggagt ttgacaacgt ctcgcggttg tacggtgacg
     3721 tgcgcgcagt agatggcgtc agtattgcga taaaagatgg tgagttcttc tctatgctgg
     3781 ggccgtccgg ctccggcaaa accacctgcc tgcgcctgat tgctggcttc gaacagcttt
     3841 ccggcggggc tatctctatc tttggtaaac ccgccagcaa tctgccaccg tgggagcggg
     3901 acgtgaatac tgtctttcag gactacgcgc tatttccgca tatgtcgatt cttgacaatg
     3961 tcgcctatgg gctgatggtc aaaggcgtga ataaaaagca gcggcacgca atggcgcaag
     4021 aggcgctgga gaaagtggcg ttggggtttg tacagcaacg taaaccgtca caactttctg
     4081 gtggtcagcg ccagcgggtt gctatcgcca gagcattggt gaatgaaccg cgcgtattgc
     4141 tgttggatga accgctcggc gcactggatc tcaaattgcg tgagcagatg cagctggaac
     4201 tgaaaaaact gcaacagtct ctcggtatca cttttatctt cgttacccac gatcagggcg
     4261 aagcgttatc gatgtccgat cgtgtggcgg ttttcaataa tggacgcatt gagcaggtcg
     4321 attccccgcg cgatctctat atgcgcccgc gcacgccgtt tgttgccggg ttcgttggta
     4381 catcgaatgt ttttgatgga ctgatggcag agaaactttg tggcatgacg ggaagcttcg
     4441 ccctgcgacc ggaacatatc cgcctcaaca cccctggtga actgcaggcc aatggcacga
     4501 tccaggcggt gcaatatcag ggcgcggcaa ctcgttttga actgaaattg aacggcggtg
     4561 aaaaactgct tgtgagtcag gccaatatga caggcgaaga actgcctgcc acgctcacgc
     4621 ccggacaaca ggtgatggtt tcctggtcgc gtgatgtgat ggtgccgctg gttgaggaga
     4681 ggtgaatggc gatgaatgta ttgcaatcac cttcacgtcc aggtctgggg ctgtttttac
     4741 tgctgcttgg cccgctaatg tggtttggca ttgtctattt cggctcgctg ctgacactgt
     4801 tatggcaggg attttatact tttgacgatt tcaccatgtc ggtaacgccg gaactgacgc
     4861 tggcgaatat ccgtgcgctg tttaatccgg cgaattacga catcattctc cgcacgctga
     4921 ctatggctgt ggcagtcact atcgccagcg ccattctggc ttttccaatg gcgtggtata
     4981 tggcgcgcta taccagcggg aaaatgaaag cgtttttata ttgcggtaat gttgccgatg
     5041 tgggcgagct acattgttaa agcctatgcc tggacgttat tgctggcaaa agatggcgtg
     5101 gctcagtggt ttttacaaca tcttgggctg gaaccactgc tgactgcgtt ccttacatta
     5161 cctgcggtgg gcggaaatac gctgtcaact tccgggctgg ggcgctttct ggtgtttctc
     5221 tatatctggt tgccgttcat gatcctgccc gttcaggcgg cgcttgagcg tttgccgccg
     5281 tcattgttgc aggcgtcggc tgatctcggc gcacgtccac gacaaacctt tcgctatgtg
     5341 gtgctgccgc tggcaatccc gggtattgcc gctggctcta tctttacctt ctcactcact
     5401 ctgggcgatt ttatcgtccc gcagctggtt ggtcctccag gatattttat cggcaatatg
     5461 gtttattccc agcagggggc gattggcaat atgccgatgg cggcggcatt caccttggtg
     5521 ccgattattc tcatcgcact gtacctggcg ttcgtgaaac gtctgggagc gttcgatgca
     5581 ctctgaacgc gcaccgtttt tcctcaaact ggcggcctgg ggcggcgttg ttttcctaca
     5641 ttttcccatc ctgataatcg ccgcctatgc gtttaacact gaagatgcgg cgtttagttt
     5701 tccaccgcag ggcctgacgc tgcgctggtt tagcgtggca gcacagcgta gtgatattct
     5761 tgatgccgtg acactgtcac ttaaagtggc ggcgctggcg acattaattg cgctggtgtt
     5821 agggacgctg gcagctgccg cgctgtggcg acgagacttt ttcggcaaaa acgccatttc
     5881 gctgttactg ctgctgccca ttgcgctgcc gggcagcgtc actggtctgg cgttattaac
     5941 cgcctttaaa accatcaatc tggagccggg atttttcacc atcgtggtcg gtcatgcgac
     6001 tttttgtgta gttgtggtgt ttaacaatgt catcgcccgt tttcgccgca cctcctggag
     6061 tctggttgag gcgtcaatgg atcttggggc caatggctgg caaaccttcc gctacgtagt
     6121 gttgccgaat ctcagttcgg cgttact
//
LOCUS       DAJVQG010000192         6073 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJVQG010000192 DAJVQG010000000
VERSION     DAJVQG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.192"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..301)
                     /locus_tag="OQM83_003655"
     CDS             complement(<1..301)
                     /locus_tag="OQM83_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCS1521812.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPLLLFKNLHREFGFTLDGAATEHD
                     TLLPRFTNDISRQSWVSEKVFCNLPYSDIPSFLLKASEADLVAFLIPHRANTSYWL"
     gene            817..1386
                     /locus_tag="OQM83_003656"
     CDS             817..1386
                     /locus_tag="OQM83_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1521813.1"
                     /translation="MRTFLYARVSVDDLTTQNQVEAVKRAGYEVRPSRVIEEVISGAT
                     PAMDRPQFVKLVDKLEEGDCLVVTKIDRLGRDNIDVQKTVTMLLGMGVKLVCLDLPVQ
                     DLSSAEGKLILQLFSTFAEFELNRIRERTREGLERAKAQGKVLGRPVAKNTTEAVLEH
                     KAKGLSQSQVAKLLNLSVRTVSRHWTKTL"
     gene            2114..2287
                     /locus_tag="OQM83_003657"
     CDS             2114..2287
                     /locus_tag="OQM83_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCS1521814.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(2545..3873)
                     /gene="rutG"
                     /locus_tag="OQM83_003658"
     CDS             complement(2545..3873)
                     /gene="rutG"
                     /locus_tag="OQM83_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCS1521815.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(3894..4388)
                     /gene="rutF"
                     /locus_tag="OQM83_003659"
     CDS             complement(3894..4388)
                     /gene="rutF"
                     /locus_tag="OQM83_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCS1521816.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(4399..4989)
                     /locus_tag="OQM83_003660"
     CDS             complement(4399..4989)
                     /locus_tag="OQM83_003660"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCS1521817.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(4999..5799)
                     /gene="rutD"
                     /locus_tag="OQM83_003661"
     CDS             complement(4999..5799)
                     /gene="rutD"
                     /locus_tag="OQM83_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCS1521818.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(5807..>6073)
                     /gene="rutC"
                     /locus_tag="OQM83_003662"
     CDS             complement(5807..>6073)
                     /gene="rutC"
                     /locus_tag="OQM83_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCS1521819.1"
                     /translation="NVLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWK
                     NYAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
BASE COUNT         1632 a         1558 c         1474 g         1409 t
ORIGIN      
        1 taagccagta gctggtgttg gctctatgcg gtatcaggaa agctactaaa tccgcttcag
       61 acgcttttaa gaggaacgac ggaatatcgc tgtacggcag gttacagaag accttttcac
      121 taacccatga ttgacggctt atatcattgg tgaatcgtgg taacagcgtg tcatgctcag
      181 ttgcagcacc atctagcgta aaaccaaatt cacggtgtaa gttcttaaat aacaacaatg
      241 gagttctcca gtaatcagaa cccccatgat gtttcccgat taaaatatgc tcaattgaca
      301 taagaaataa aaaattttat tgcaatcact cttgacaagg tttactctct tttaagataa
      361 gagatactta acaggctttc taaacggata tcctttctgg aatatctatc tcttatctta
      421 aaagtcttta cagatacttt aaagatatct gtattaaatc tgtacagtaa cttttcagaa
      481 tctgaaaatt tttataaaat cgtagtagcc cctcttcaaa ttccccttct aaaaatcttc
      541 gaaaatatct gacagatgtc tgtagctgct aagtacccgt cacaccagct atgtgacccc
      601 ctttgcaaaa aagccaaata tcattgactt tctcggtgaa ttgctataaa ggcatctatc
      661 taggtcttta actgtataaa tacacatgac aacaaacgcc cgttaagtgt ctatataaaa
      721 aggtagctgt agctatagac aacaattgtg ataattgatt taatggctat agacaagcaa
      781 gacagacctt tgttgactat atagagagat aagaacatgc gtacattcct ttatgctcgt
      841 gtatcagttg atgacctcac cacacagaac caagtagaag ctgttaaacg tgctggttat
      901 gaagtcagac cttcacgagt aattgaggaa gttattagtg gggctacacc agctatggac
      961 agaccacaat ttgtaaagct ggtggataag ctggaagaag gtgattgtct tgtagtgacc
     1021 aagattgacc gcttaggaag ggataacatt gacgtacaga agaccgtcac aatgttactt
     1081 ggtatgggtg ttaagctggt ctgtctagat ttgcctgtcc aagacctttc aagtgctgaa
     1141 ggtaaactta ttcttcaact attcagtacc tttgcagagt ttgaacttaa ccgcatcaga
     1201 gagcgaacca gagagggctt agaacgagct aaagcacaag gtaaggtgtt gggtcgtcct
     1261 gtagctaaaa acactacaga ggccgtttta gagcataagg ctaagggatt gtcacaaagt
     1321 caggttgcaa agcttctgaa tctctctgtg agaacggtta gccgtcactg gacaaagaca
     1381 ctgtaaaaaa aacttagtag catcttcata gcctccttca tgggggcttt tttgtgtctg
     1441 taacatatgg tgaccaaaca tctatatatc gttatatagc cctgtatata gcactatata
     1501 tagtgtctgg agagggacga ctaacaccct gcatagttcc ctgaaaaggg gcttcacaga
     1561 ttctctatat atttcaataa gttaggataa aaagggacta taccgatact gcatagaatc
     1621 tgtgtaggat tgcatagtca taggggttgg aacaagccca actacacctg ataaagcctg
     1681 ttattaacag gtagttgaat gtacgtgttt agctatgtaa aagcaccaga actttagcca
     1741 tttctaacca ctcctcgtgt tatctctatt ccgtagcgat tcgctaccac ttatttaaag
     1801 ataagacgtc cttttcagag tgcaaatttc acaaccactt atttgattta taacaacttt
     1861 cacaagcacg taattttgtc gcaaaatgac acatttttat ctcatcgcgt ttttttaatc
     1921 ataagagcgg cttatggata attattggag atgatatcta ttctcgctaa gaagctgttg
     1981 caggatatta ccaaacgcgg gtctacccgc gtcagttcac taagcttagt cccacgtagc
     2041 gaaaatatgg cagccgccat acgccgcgtt aattctatgc aatatgatgt ctatacccag
     2101 acggaggtca gtaatggcaa accatcgagg cggttccggc aattttgcag aagaccgcga
     2161 aagagcatca gaagcaggta aaaaaggtgg acagcacagc gggggtaatt tcaagaatga
     2221 cccgcagcgc gcatctgaag caggtaaaaa aggtggcaag agcagtcacg gcaaaagcga
     2281 caactagccg ggctaatcaa tgacgaatgc atttttgtct gtagctcgtc aaaaagccat
     2341 caccgccggt tacccggtgg ttgatactga tgacaaatgt aagcttgcct gatgcgcgat
     2401 gcttatcagg cctaccagaa gattgcaata tattgaattt gcactgtttt gtaggccgga
     2461 taaggcgttt acgccgcatc cggcatgaac aatgcgtacg ttgtcaacaa tctgcaccgc
     2521 cgggtaaccc cggcggtttt ctgtttatgg ctcctgatga acaacttctg gcggtggaac
     2581 gtcaaccaac cttctactta acaacgcatt gagtaaaatc gcgccaaagg tggctgtacc
     2641 gatccctccc aacgtaaaac cgcccagcgt gagagcaaaa tcacccgcgc ccagcactaa
     2701 ggttactgcg accataatca aattaccgtt ctggcttaaa tcgacacggt tttgtaccca
     2761 gatccttgcg cctgcgacgg caatcagccc gaacacaaca attgatgcac caccaatcac
     2821 cgcggccgga atggtatgga tcagcgcgcc aaatttcggc gaaaagccca acagcatggc
     2881 gatgacggca gcagcaacaa acaccagcgt cgagtagact tttgtcacgg ccatcacacc
     2941 gatattttca gcataggtgg tgacgccgct accgccgaca gagccggaaa gcatcgttgc
     3001 cagaccatca ccgacgaatg ctcgccccat atacgggtcc atattgcgtc cggtcatccc
     3061 ggcgaccgcc ttgagatgac ctaagttttc cgccaccaga atcaccgcca cgggcgcaat
     3121 cagcatcatt gcctgactat taaaagcagg agtggaaaaa tgtggcaaac cgaaccaggc
     3181 agcatggctg acgagagtaa aatcgacggc ttttcccagc cctaaaacgt tggtcatcac
     3241 gccatacagc agacaggcga caattaaacc tacgagaatc aataaccgct ggatcatgcc
     3301 acgggtaaac accgccacca gcccaataca cagcaccgtc attaccgcca tccagctatc
     3361 aaaggctgaa gccgatacac ttttcactgc gataggcgct aagttcaggc caatcgccat
     3421 caccaccgca cccgtcacca ccggcggcat tagtcgttca atccagcgtg taccaatttt
     3481 catcaccacc aggccaatga cggtataaac cagcccacag gcgataatcc cgcccagtgc
     3541 gatgctgata ttcgggttaa tgccctgacc gttaaagccc gtcgcggcaa tcaccacgcc
     3601 gacaaaagcc gcgctggagc cgagataact ggggacgcgc ccgccggtaa taaagaaaaa
     3661 cagtaacgtg ccgatccccg acattaaaat ggaaagattg ggatccagcc ccatcagaat
     3721 cggcattaac accgtcgcgc caaacatcgc caccgcgtgt tgaacgccca ttactgccgt
     3781 ctgagcaaac ggcaatcgtt catccggcgc gaccacgccg ctctctgtag aggtcgattt
     3841 taactgccag tgaggaaaac cgaacattgc catcagctgt ctccttaagg aggttaacaa
     3901 gcagggcgca tcagcgcgtg ataactgcga tcgaaccaca ccagcccgta gggtgtggtg
     3961 tgacgatgaa tcgcttcgat ggcgcaaaac agaatgtcgt gggtgccgac gctcaccacc
     4021 tggctgatgc ggcagtcaaa cgaaaccaga gcctcttcca gttgcgggca tccggtcaca
     4081 cccgtctgcc agcgggcggc ggcaaagcgg tgttccatgg gcgttttgcc gccaaaaaga
     4141 tttgaaagcg gctcctgccc ggcgctcagt gtatttacac acagcgttcg attttcattg
     4201 aataccggcc agacggacgc cccacgattc aggcacacca gtaatgtggg cggcgtgtcg
     4261 gtcacgctgc agacggcgct ggcggtgaac ccggcgcgcc cggatggacc gtccgtggtg
     4321 atgatattga ccgccgcgcc catacaggac atcgcatcgc gaaaggtttg ttgatcaact
     4381 atgttcatag tttgctcctt acaacagccc gcaggcttct tcaaaggaca gacgtggcag
     4441 gcgcgcaaaa agtttgctgc tatcgccata gccgatatta atcagcagat tgctcttcag
     4501 cgtgctgccc gcaaaaaagg cgtcgtcaac gtgttgacgg tcaaaacccg acatcgggcc
     4561 ggtatccagt cccagcgcac ggcaagcgac gatcagatag gccgcctgca tggagctgtt
     4621 gcgaaacgct gtttcttcgg caagttgtgg gctggaggta aaccaactgc gggcatcacc
     4681 gtggggaaac agtagtggta accgttcata aaattcactg tcccaggcaa cgatagcggt
     4741 gacgggcgcg gtcagggttt tttgtagatt gccgctggaa agcgccgggt gcagacgttc
     4801 ttttccttct acagtgcgga taaacacgat ccgtgccgga gaacagttag ctgatgtcgg
     4861 cccccatttt atcggggcat aaagctcccg taacgtctca tcgctgacgg gtgtctcccg
     4921 ccagccgttg tgagtgcggg catcggtgaa cagggtgcta agcgcacctg ggctaacggc
     4981 ttcgttcata gcaattcctt acagggcggc ttcacggtga tgtaacaggc tggcaagccc
     5041 gttgagtaac agagcattaa acgtttcggg gtcggtcacg ttgcaggcgt gtccgccata
     5101 gcgcatcacc attttctggc tatcgggcag ggcggcatga agctcactgg aacatgctga
     5161 tggcaccagc agatcatcac tggcgcagat gatttgcacc gggcagcgga tgcgatccgc
     5221 atggtgacta aagtcagcgc gtttgagggc gttaagtcga cgcagtaaat tatttttgcc
     5281 ctgaaaatgc gccagtgcca gcgcgtcttc tgcctcaagg cgaggtgcgc gggccgccat
     5341 ccagtcggcg ggatagagga acaacggctg cgcttccacc catgcctgcg cgccgccgct
     5401 atacagtaat cgttcgcgaa cctgaaaaca gcggcgcgta tgggcgttta ttcgtagcca
     5461 gccgttaacg ctgatcagca cagttaccga cgcgggataa tccagcgcca gctgcattcc
     5521 caccagcgca ccgagcgcat ggccgaccac cgcgtaatgc tcaatccctg cggctaccag
     5581 cgcctgatgc agttccgctg ccatctgggc gatactgtaa tcttctgcca gcgtgtcggg
     5641 attattgccg gtgccgcgct ggtcgtaaca gactacctga tactcctgct ccagcaccgc
     5701 cagttgcggt aaccagtaac tgccgctacc gccaagaccc gaaatcaaca ccactacggg
     5761 cgcatcagca taagggggag gtgagagtga aagtttcatc gcggcctcac ttggcgatat
     5821 gcgcaattgt ggcgatttcc accagcgcgt caggttttac cagtccgcac tgaatgcaga
     5881 atcgcgccgg tttatcaccc ggaaaaaact cggcgtagat ttcgttaatc gcggcgtaat
     5941 ttttccagtc ggtaataaag atgctgttga aggtcacatc cgccatcgtg ccacccgccg
     6001 tctcgatcac cttgcggata gtttccagaa cgtggcgggt ttgcgccttt gggtcatcgg
     6061 caaacagcac gtt
//
LOCUS       DAJVQG010000193         5956 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJVQG010000193 DAJVQG010000000
VERSION     DAJVQG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.193"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            1..509
                     /locus_tag="OQM83_003663"
                     /pseudo
     CDS             1..509
                     /locus_tag="OQM83_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(551..1003)
                     /locus_tag="OQM83_003664"
                     /pseudo
     CDS             complement(551..1003)
                     /locus_tag="OQM83_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1067..1213
                     /locus_tag="OQM83_003665"
                     /pseudo
     CDS             1067..1213
                     /locus_tag="OQM83_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1213..2370)
                     /gene="intS"
                     /locus_tag="OQM83_003666"
     CDS             complement(1213..2370)
                     /gene="intS"
                     /locus_tag="OQM83_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCS1521820.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2532..2606)
                     /locus_tag="OQM83_003667"
     tRNA            complement(2532..2606)
                     /locus_tag="OQM83_003667"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2571..2573),aa:Arg,seq:cct)
     gene            complement(2682..3614)
                     /locus_tag="OQM83_003668"
     CDS             complement(2682..3614)
                     /locus_tag="OQM83_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCS1521821.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3908..4663
                     /gene="mlaA"
                     /locus_tag="OQM83_003669"
     CDS             3908..4663
                     /gene="mlaA"
                     /locus_tag="OQM83_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCS1521822.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(4845..5180)
                     /locus_tag="OQM83_003670"
     CDS             complement(4845..5180)
                     /locus_tag="OQM83_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521823.1"
                     /translation="MAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDD
                     AKFRRDNVKEIISNLFANKVDVDHPENKYKDFKDLEDNVEKRLQNRQAKYQNEINQLS
                     APGVNFDDI"
     gene            complement(5138..5530)
                     /locus_tag="OQM83_003671"
     CDS             complement(5138..5530)
                     /locus_tag="OQM83_003671"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521824.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLF
                     TWYGKDKKGNSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARN
                     ASAMLQRGLKAVKLSWQNHLQQVPIIRL"
     gene            complement(5537..5913)
                     /locus_tag="OQM83_003672"
                     /pseudo
     CDS             complement(5537..5913)
                     /locus_tag="OQM83_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937824.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1485 a         1337 c         1312 g         1822 t
ORIGIN      
        1 tatttaaaaa taaactttgc tgacttcagt ttaattaaag gattaattag ttgggctaat
       61 cactgtgctg aatacataga aatttttgat gagtctattg cttttacatg tctctctgca
      121 ttttcttctg aaaaacaatt tggagtattt ctgtttggat gtttgaaaag cacagggcta
      181 aagttaaaac gattattcat acggatttat ctgcaccatg gcgtcttaag gatatatcat
      241 caagattata tctcagcgaa agtttactaa agatgaaatt gaaagaagag ggggtatcat
      301 tcagtaagat catacttgat gagaggatgc aaatggctga atatttactc agcactcgtt
      361 gttatcctat tagtaaagta gctaaggtct gtggttatgc cagtgtctca tactttactt
      421 atgtatttag acgttatttt ggtgtttctc caagtcaata ctctcagagg agttcagaaa
      481 gtaaaattct tactcaccag ggaatctgat cattgttctt gcccccttat ttccagacag
      541 ggggtgtatc ttaagttaac gttacccgct gacgtcgata ttctcgcgga gagcgataac
      601 ccagcgcact gtgcggatgg ttttcattgt aatgttcgat cgccactgca agattatgca
      661 atgccgttct tacattcggt ttcggcatga acgcgatgta gtcttccttc atcgttttca
      721 cgaacctttc tgccattcca ttactctgag gactgctgat tgccgttgtg cagggctcca
      781 gattcaactc tctggcgaac tgccgtgttt catgcgctct atacgctgaa ccgttatctg
      841 tcagccactg gatggactgt tccggtactt tatcgccaaa gcgtttttct atcgctccta
      901 acatgacatc ctgcactgtc gctttatcgt aacctcccgt gcttgcggcc cagtctatgg
      961 cttcacgatc gcaacagtcc agggcgaacg taacacgcag ttttgaatcg ccacgggttt
     1021 aacagacacc tcagagtcat ttaagatggc ttaaagagag gtgcccatga gcggtaagcg
     1081 ttatcccgaa gagtttaaaa ctgaagcagt caaacaggtt gttgatcgcg gttattctgt
     1141 tgccagcgtt gcaacacgtc tcgatatcac tacccacagc ctttatgcct ggataaagaa
     1201 gtacggtccg gattactcca ctttttcatc aagccaatcc gcccaccact gcatcatttc
     1261 tctgcgctta tcgagatact gagcatggtt gtaaataccg cgcacagatc cgccgttggc
     1321 atgtgccagc tgcacttcaa tagcgtcagc aggccattcg tgctcgttca taatcgtgct
     1381 gaattcatgc ctgaatccgt gaccgctttc cagaccttca tagccgattt gtttgatcac
     1441 aagcaatacc gcgttctcgc agattggctt cttcttatcg ttgcgcccgg caaaaacaaa
     1501 ctctgagact ggtttggtga ttgagcttag cgtagtgaga agttcaacca cctggtctga
     1561 catcgggacc acatgaattt tgcgtccctt catcacactg gcgtcgatgg tgataatcct
     1621 gttttcaaaa tcgacgttct tccatagcat ggaacgaagc tcttttgttc ttagggctgt
     1681 gtagcgtaaa actttggtcg caatgagcga tacgatactt cctgaaaatg ttgccagtgc
     1741 tttgttgaat gcaggcatct ggtctgctgg aaggaacggg aagttcttct tgcggtatcc
     1801 tttcatggcg tctgcaaggt caggtgccgg gttatattta gcccttccgg tgacaatagc
     1861 gtaacgaaaa acctcgccgc atcttctgcg ggctttgttg gctcgctcca ttgcaccgcg
     1921 atcttcaaat ctgcggatta cttccagcag ttgcatcggc tcaatatcct gaatctcaag
     1981 accgccgatg atgggtaaaa tgtcgtcatc aaacattttg gcaagttcag ttgcatagcc
     2041 tactgaccag acttgcttct tgtgctcgta ccattccttg taaatcgcac taaatgaatt
     2101 gttgttagac gaagcctttt tcgcctttac cggaacgatg ccaaccgaga tgtctttcct
     2161 cgcagtccat gccttatccc ttgcttcctg caaagtcata agcggatatt ttccgacggt
     2221 caggattttc tccttaccgt caatcttgta gcgaagctgc catacctttt tcccggatac
     2281 aggaacataa aggtacaggc cattaccatc gagaaggcgg tatggttttt ctttcggctt
     2341 tgctgcttca atctgcttaa cggtgagcat gggtaaaaat ccggtgggta aaattatttt
     2401 atccactttt tacccgtcat ggagtgcggc tgtcaacgat ctgacgcgaa ccatgacgaa
     2461 ccgtgaatat acggaaggct tgatattcag gggattttgc ggactggtac ggatgggagc
     2521 gaactgataa atggtgtccc ctgcaggaat cgaacctgca attagccctt aggaggggct
     2581 cgttatatcc atttaactaa gaggacaatg cggcatgagt atacccgcta atggagtgcg
     2641 gggtaagtac gctgccgctc gattgcttaa accctcgcca tttatgccgg gtttttatca
     2701 tttttcttaa tgttttccgc acgttctgct ttttggcgtg cttctgcttt acgcttgttg
     2761 ctcatgtcgt tacgaatctg tgcatgactc attaacgcga agataaaggt gccgccgcag
     2821 atgttccccg ctaaagtagg tagtgcgaag ggccagatga aatcgctcca gtgcagcgta
     2881 ccgttaaaca ccagatagag gatttcaaca gaaccgacca cgatatgggt ggtgtcacct
     2941 agggcaataa gccaggtcat caatataatc accacaatct ttgccgcacc tgctgcagga
     3001 aacatccata ccatagtggc gatcagccag ccggaaatga tcgcgttggc aaacatctcg
     3061 ctgggggtgt tcttcatcac atccatgccg attttgacaa atgcatcgcg agtttcttca
     3121 ttgaagatag gcatatattc aaatgcccac gcagcaatac cagtcccgag aatattaccc
     3181 agcagcacga ctccccataa ccgcataagt aagccaacgt tgctcattgt cggtttttgc
     3241 atgacgggta gtaccgcagt cacggtgttt tcggtaaata attgctggcg ggccattatg
     3301 acgataataa aaccaaaggt ataaccgaga ttctccagta agaagctgcc cggcacacct
     3361 tccagttcga catgaaatat cccttttgcc agtaacgaag cgcccatcga cagacccgcc
     3421 gcaatggctg accacagtag cgccattgcg tcgcgttcca gctctttttc accatcctgg
     3481 cggatatgct catgaattgc catcgcccgg gaggggagtc ggtcttcatc tatttctatt
     3541 tttttgccgc gctctttttc ttcgctctca acttcaattt cgtcgctgtg ttgatcaatt
     3601 ttgtcgttgt ccatggtctc ttctcttgaa ttagcacgta tagctaaagc gtagcggctt
     3661 ttttgctcgc aactggcggg agttactctg aaaatgtaga aaaggctgcg tttgcctttt
     3721 tctgtttcta tagaatcaag tagcctacag ggcggcgatt accaggctat gatcaaatca
     3781 gcaaatcagg gcgtctggac atcagttgac gtgctgttac aatcgcccac acctaaacag
     3841 gcggatacgg tatcgttccg tcatggatgg caaactgcat aagccataaa aaaacaggga
     3901 gacatttatg aagcttcgcc tgtcggcgct tgctctggga actacgcttc tggtggggtg
     3961 tgcgagttcc ggtacagatc agcaagggcg ttctgacccg ttagaagggt tcaaccgcac
     4021 catgtacaac ttcaacttca acgtattaga cccgtatatt gttcgaccgg tcgctgtcgc
     4081 ctggcgtgat tatgttccgc aaccggcgcg taatggtttg agcaacttta ctggcaacct
     4141 tgaagaacct gcggtgatgg ttaactactt cttgcagggc gacccttatc aggggatggt
     4201 ccactttacc cgctttttcc tgaacaccat tttggggatg ggcggcttta ttgatgttgc
     4261 agggatggcg aacccgaaac tgcaacggac tgaacctcac cgcttcggta gtacgcttgg
     4321 tcattatggc gtgggttatg ggccttacgt tcagttaccg ttctacggta gcttcacgct
     4381 gcgtgatgac ggtggtgata tggcggatgg tctttacccg gttctttcct ggctgacctg
     4441 gccgatgtct gtgggtaaat ggacgcttga agggatcgaa acccgcgctc agctgctgga
     4501 ttccgatggt ctgctgcgtc agtcgtccga tccttatatt atggtgcgcg aagcgtactt
     4561 ccagcgtcat gatttcatcg ctaatggcgg cgaactcaaa ccgcaggaaa atccgaacgc
     4621 acaagcgatt caggatgatt taaaagatat tgattctgaa taagaaacaa ataaaaaagg
     4681 tgagtcgcaa aactcacctt ttttgttgtt atccctcaat taccacaagt aaaaggagat
     4741 aaaccgacaa tgacccggac gtaatgatgt aaaaatactg atgttcgtca ctgactattt
     4801 ccgatattca ggcgttgaaa aatcaaaaaa ctggaaaaat aattttaaat atcatcaaaa
     4861 ttaacacctg gcgcagatag ttgattaatt tcattttgat atttcgcctg gcgattttga
     4921 agtctttttt caacgttatc ttcgagatcc ttaaagtctt tgtatttatt ttcaggatgg
     4981 tcaacgtcaa ctttgttagc gaataaatta gaaattattt cttttacatt gtcgcgtcta
     5041 aattttgcat cgtccacttg aaaattatat cttattgctc gacctaaacg tttaaaaact
     5101 tcgttaggtt ctctcagtct ttccccgttg agctcgttta caacctgatg atcggaacct
     5161 gttgcaaatg attctgccat gatagcttta ctgctttcaa accacgctga agcattgctg
     5221 aagcattcct ggctttttca ctacaatatt gggtgagact atcaccatta atacgaattc
     5281 ctctttgaat gtggtagtcg tcctgatcgt agtaaccgag agataagtaa tcatttccgt
     5341 ttttattttt aattacaata gcgagagagt tgcctttttt atctttaccg taccaggtaa
     5401 ataagtccgt tggttcgcta ttcccgctct catcaaattc tgttaccgta tgtctgagtt
     5461 tgtgtgatat cttatcgaag ataaaaccag gatctccatc atatatttct tttaatgcgc
     5521 tgaagatcat ttccttgtta ttgatatcat gagtcttctc aactatgttg tcattaatga
     5581 tagtacggac gagttctttg tctttatagt tcgcaggtaa ttctattggt tcttggttgt
     5641 cgataccaat agtaatatgg tctgtatcat tggagccaat atcaatataa gtaactgagg
     5701 aattattaat attaaatctt aaggatcgtc gtttactcgt atggcgtttt ttataagctc
     5761 aagtagatgt ttaatacagg tgcttattcc ttccttacca ttaaaaaggt ttttgagctc
     5821 ttgtactaat ccattaactt catcattgtt attttcatta atggattctg ggtaagttgc
     5881 gctggtattg ttgattgaaa tagatggtag catgatttta ttccattttg attgatttgt
     5941 tacaaatata tattac
//
LOCUS       DAJVQG010000194         5921 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJVQG010000194 DAJVQG010000000
VERSION     DAJVQG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5921
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.194"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            282..358
                     /locus_tag="OQM83_003673"
     tRNA            282..358
                     /locus_tag="OQM83_003673"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:316..318,aa:Arg,seq:tct)
     gene            complement(403..1164)
                     /gene="envY"
                     /locus_tag="OQM83_003674"
     CDS             complement(403..1164)
                     /gene="envY"
                     /locus_tag="OQM83_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCS1521825.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1347..2237)
                     /locus_tag="OQM83_003675"
     CDS             complement(1347..2237)
                     /locus_tag="OQM83_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCS1521826.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2238..5210)
                     /gene="nfrA"
                     /locus_tag="OQM83_003676"
     CDS             complement(2238..5210)
                     /gene="nfrA"
                     /locus_tag="OQM83_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HCS1521827.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5197..>5921)
                     /gene="nfrB"
                     /locus_tag="OQM83_003677"
     CDS             complement(5197..>5921)
                     /gene="nfrB"
                     /locus_tag="OQM83_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HCS1521828.1"
                     /translation="DTALRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESID
                     AWQIPSSLIAEMPASVALHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRY
                     FIVLRGQIVTGLRHWYARRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAE
                     ILTTLGHINRSAINVLLLRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQS
                     LLLKAGLNTEQVAQLESENEGE"
BASE COUNT         1345 a         1558 c         1548 g         1470 t
ORIGIN      
        1 aggatgagta tccataatga tcaccgacgt tggtttcata gttaccagtc tcataggagc
       61 ggacaatttt ccgttagggc ggtaaattgt actttgatac atgaaaatac gggttttctt
      121 gattcaaacg cgcagcggtg tgcatttgtt tgccgctata gcgtaataaa tcagaaaatc
      181 agacgcggtc gttcacttgt tcagcaacca gatcaaaagc cattgactca gcaagggttg
      241 accgtataat tcacgcgatt acaccgcatt gcggtatcaa cgcgccctta gctcagttgg
      301 atagagcaac gaccttctaa gtcgtgggcc gcaggttcga atcctgcagg gcgcgccatt
      361 tcttcttcat ttaaccccgt cttatccgtt ccgctttgct cttcaccaca tcactttttg
      421 tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa aaacagagat
      481 aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat ttttgttatc
      541 catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct ggctatagct
      601 gatattttcg ttttttaatt tctttttgag caggctgggg cttaaacata gcgaactggc
      661 gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc agacgctgtc
      721 gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt gctcaagaaa
      781 gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg gtttgctggg
      841 ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc tatgacaagc
      901 gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaagttct tatttaaaaa
      961 caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt gatttaacgt
     1021 tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt tcggaagggt
     1081 aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga tcaccacgca
     1141 aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact ccgctcaaag
     1201 gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt ctttctttag
     1261 cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag gtaagccagg
     1321 tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc gacgggcaaa
     1381 tagcgcagag agaaatagag agtgtcttta ccgcaagagg aacgtttcgc taacagcgag
     1441 gcgatctctg catccggttt cggtttcgct gtaaaggttt tgcctttgcc ggcgacaaac
     1501 agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct ggcctgtaga
     1561 taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg tacccagaca
     1621 ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatctgg cgacatgttt
     1681 ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat cagccgctgc
     1741 gcgttgttta accatgttag caaaggctga cgggcagctt cgctgcgcca gttcaggtcg
     1801 tcaatttccg cgctgatgta ccagccatcc ggcgttatgc caggcgcggc gctccataat
     1861 ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag cgctgcggac
     1921 gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat cagtttcagg
     1981 ccagcctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc tggctgggta
     2041 aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg caaacgtaac
     2101 tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt ttgtggttgc
     2161 cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt cagcaatacg
     2221 aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt gttgcgctct
     2281 ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag actgactttg
     2341 tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac cccgactccg
     2401 ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac gtgagcgtaa
     2461 ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata atctgccgtc
     2521 cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt ttgggcaaac
     2581 cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa gaatgaggcg
     2641 ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa ctgctgttcg
     2701 acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt gccggacatc
     2761 ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac gcggctataa
     2821 actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc ggcttccagt
     2881 tgcccgtagc tacgatagct tttccctggc gctgcgccgc caacattatt gttagcggta
     2941 ctcattgccc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca gcggcgaccg
     3001 acctcctcat gcaaacggcg gaaattgaag cgttgttgat tttgctctgg ggtcagtggg
     3061 gttatcagag cctgattatc aatgtcatca atcaccagcc gggcgtagtg ctgcgtcgca
     3121 ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc cggatcgtcc
     3181 ggaagccttt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc accgctatcc
     3241 cacaaggcgt aaccaagcgc tgcctggatg ttgctattat tcggttccag ttccagcgcg
     3301 gcgcgcaaat cactcaccgc ggccgggaca ttatgacgtt ggcgataaat tgtcgcccgc
     3361 gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc gttcagtgcg
     3421 agttccggct gaccaggaat gtaacgttgt gcatgcagcc accagtagag ggcattgctt
     3481 cccagtccac gttgttctgc ctgttgtagc cagcgatcgc gagccgcacc atttcctgcc
     3541 gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg aagactgatt
     3601 ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg ataggctacc
     3661 gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc atacaacgcc
     3721 acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc agcatcgtag
     3781 gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc aatacccggc
     3841 aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa aatcgccacc
     3901 ttcgccggcg ttgccaggta aggatgactt tccagcagag acgccagtcg cgccattaaa
     3961 gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa atcggcagct
     4021 tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg ggtaagattt
     4081 gccggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt gttacgggtc
     4141 gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt ggcgagcagt
     4201 ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc gccaacaaca
     4261 tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac ggggttggcg
     4321 ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt aaacagcggc
     4381 ttattttcat tgagataatg ctggaggcgt gctttttcgc cacgataagc cagcgcggtc
     4441 gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag tgctaggatc
     4501 cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc tgccgcgctt
     4561 aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca ttgtttcagg
     4621 tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga tgcagcaaac
     4681 gtcgcatcgt tcagttgcgc tctggcgaca ggtaactgtg caagccgcag ggcattctgc
     4741 ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg ctgggcaagc
     4801 agttcttcaa cagttgtaac gcttttcact tcaaccggaa tagccgccag actgcgctca
     4861 agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg cgcccggtca
     4921 tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat attatccggc
     4981 acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt atttttctgt
     5041 gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc gctcagcccc
     5101 agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa taaagacgtc
     5161 agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat tttcggactc
     5221 cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt gcatcgaaac
     5281 ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga taacgccttc
     5341 ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata acaacacgtt
     5401 aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca ggaactgatg
     5461 cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact gatgctgaac
     5521 cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc agtgacgtaa
     5581 ccccgtgaca atttgtcccc gcagaacaat gaagtaacgc actttgcgtc cgactttacg
     5641 cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa ttaactcatc
     5701 attttccaga cgcagcggca gtaccgcata atgcagcgcc acggaggccg gcatttcggc
     5761 aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc cgttttgctc
     5821 tgccagcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca ttgaaccgcc
     5881 caagcgtaga ccttcgacgc gattacgcag tgctgtatcg a
//
LOCUS       DAJVQG010000195         5837 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJVQG010000195 DAJVQG010000000
VERSION     DAJVQG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.195"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..242)
                     /locus_tag="OQM83_003678"
     CDS             complement(<1..242)
                     /locus_tag="OQM83_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1521829.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(604..3387)
                     /locus_tag="OQM83_003679"
     CDS             complement(604..3387)
                     /locus_tag="OQM83_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCS1521830.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(3444..5816)
                     /locus_tag="OQM83_003680"
     CDS             complement(3444..5816)
                     /locus_tag="OQM83_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCS1521831.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
BASE COUNT         1488 a         1321 c         1311 g         1717 t
ORIGIN      
        1 ttaatggata aatccatcaa tttgtggaca ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcgt cattgataat ctggaatgcg
      121 acatcgtcgc gcatttcgtg cagcggaaaa cgttttgatt ctgcgatagt ggaaatagac
      181 ttcgcaccaa aacgtgaatc gagtagttcc gaccttaaat ccgttacttg cttcttatcc
      241 attttaaact ccttaaaatg attggatagc attaaaaaag taggatttat cgataaagta
      301 agcaagtgca aaggactcgt gtttaaataa caaaatccta atgttattta tcgtgagata
      361 ttacgagaat aatatttttt cattgaaaaa caatataata tgaaattctt gggtggtggt
      421 aaggtgtttt atgctgttat ttttatgcgc attctgtgcc tcctgaattt tcacgtaaaa
      481 atcagacctt aaaatatcac tattagtacc tggttattat tttgaacgca tttataaaat
      541 tattacataa aaatggcgaa tattgctaaa atccccgcca acgatgtgtt gacggggctg
      601 ttattatttt ggcaataata ctccagtata agtatttacc ggatgagaaa gatattgttt
      661 aacggcagtg ttaacattct ctaccgtcat ttgtttcaac aattgctcct gctcagtcca
      721 tgctgcagga tcgtcatatt gaataagact atttacaata gtgttcgcta attgttgaac
      781 gctacgctgt tggatatcga ggctgcgctg aacgttttgc tggtattcat tcagttcttg
      841 ctcactgatc cctttagcca gacgcttaac catcacttca ttcgctaacg ttaacagttc
      901 atcatgtcgt tctggttgac aagtaaaagc cagcaaatga ctgatatctt tggcctgagg
      961 atcaaccgag aggcgagaag aaacgctgta tgctccagat gcctgttcac gaatattaac
     1021 gcgtagatct tttgccagtg cgacgttaaa agcatcgagc gccatacgcg tcgccagatt
     1081 aacaggtgtc cgggaatcat aacgcttcca ctgtgaaacc tgtgccacag gttcattttg
     1141 ttcttttaca gtaaccgatg cgttgtccgt cgcgcgagtt aatggtttac ctgcggctaa
     1201 tggcgaatca gagtgtttga ttgatcctaa gtaacgcgta attaacgcca cgagtttgtc
     1261 ttctgagaca ttaccgacaa tgacaaacgt gatatccgct ggagatgaaa acaactggcg
     1321 atcggcagcc agcgcatctg cggcagtaaa ctgtacaatc tgattttctt gcagtaattt
     1381 cgtgcggtca tcagcatagc gcgtctcata catctgctgg gcgaatttct ccgctggacg
     1441 ctggtcgagc gttttcaacg cctgaatttg agcattttgt agcgatgccc aaatattgtc
     1501 gttaatcgtg ctgtgggtga ttcgctggtt aatcaactga aaaccaggtt cagggttatt
     1561 agtccgcgcg ctaacggaga gcaacgtatt catgccactg actttactgc tcatggttac
     1621 cgaattttcc gcactccagc gtttcaggct ggaagaggag agctcaccaa cgccgcttcc
     1681 gcttactgct ttatttgcca gcgcgataag tgatttttgc tgcgcaggga aacttaaatc
     1741 gcctttatta gatacggcag taatttgtag cttttgctct tcacccgcgg attttgccag
     1801 aataaccctg gcaccattgg aaagtgttaa tgatgtcaga ttctcagcca gcgtttcttt
     1861 gctgctaatt tccgcctgtg ggtcagcgtc tactgttaac gataaatttc tgcctgggaa
     1921 gatgtaagcc gccagctttt tgttggcgta ctctttttcc agcgccagga tagccgcaag
     1981 agacaatgct tttttggcgg caagctcatt gtttaccatt tgctcccaaa atgcgtcctg
     2041 gttctttctt aactgctgcc atttttccgc cagactttgc acggtaattt gctgccacag
     2101 acgtttcgaa agttgatatg tctcttccgg cgacaagaac ggcgtattat ttaatgagct
     2161 ggatgccagg cgactggtca gcatacgtaa atcacgttca gcttgctgat caaccgcatt
     2221 tttcagccag gtgaggcggg tagatttgac atcatcgagt tcttcagcag aaaaaccatg
     2281 ctgatcaatg gttgccaact ctgccattaa tgcatttgca gcatcctgca tattatcgtc
     2341 tcgtgcattt acacggaaaa acagcgactg ataatcgggt gcaattttaa cgctgcgcgc
     2401 agtgccgcca gaaatagtct tcaactcgcc cgactgtatg cgttcctgca gacgttgatt
     2461 gaacagctga actaacatgc tccattcagc ttgttcgaca aagctttgct catcgttcac
     2521 ttgtaccatt ggcaggcgat aatagagtgc gatgccgttc actcggtttt ctttatcatt
     2581 aatgatatta aagcgcaggt ggttttcggc ttttgtcggc cagacgcgat tttcagctgc
     2641 tttgttagcc ggaagcttac ttaaattatc ctttatcagc gccagcgctt ctttactgtc
     2701 gatatcgccg accacgataa aggtcatatt atttggttga taccagcgtt gataaaattg
     2761 gcgcaattgt gccggtgtga ccgtggcgac agtatccatc aggccgatag gttcacggtc
     2821 taaattacgg gtatttgcca gcaggaaagg gcggcgcgcc tgagaggtgc gccatttcgc
     2881 atcctgatgg gcacgccatt cctcagtaat tacgccgcgt tcagcgtcta cttcgagtat
     2941 ttcaaaggtt gcggcattac tccattcact gaagattgcc atcacttgtt gcagattttg
     3001 tttctgcgta gtcggcagac tcacctgata cagcgtttcg tcatagctgg tataggcatt
     3061 aacatcgcga ccgaaacgca ggcccattga ctcaaatgtt tcgatgactt tattacccgg
     3121 ccatgttttt gtgccgttaa acatcatatg ttctacaaaa tgagccacgc cgcgctcatt
     3181 gtcttcttcc tgcaatgaac cggtatgaat ttgcagccat aaatttacct gatcctttgg
     3241 ttgagcatgc ggataaatca tatatcgcaa gccattgtcc agttgcccgg taattaactt
     3301 ttcatcctgc ggtaaggcgg cggcaatcag ccgcccgggg agcaacagag ttgccactaa
     3361 cgtcagtaag aaacagaggt ttctcataat tatctccatg cgaaaaccgg gcgaatttac
     3421 ccggttaagt aaaatccgaa ctattaaaat ttcatgctga catcaagcca gaaagtacgg
     3481 ccactggcgt aggtcgccac accggtgttc gttgtatcaa cagcggtttt gctatccagt
     3541 acattgagaa tatcggcact gatcgtcagg ttttgttgtt tcagaaattg tggcgtccag
     3601 gacaagcggg tgtcccaggt caggctgcta tccagttttt cgtcaacata ctgcttgtaa
     3661 tctgaatatt cgctgatgta ttgcgcattt gtcttaccaa ggataatgcg agctttacgc
     3721 gcttcttgcc aggccagcgt attcgcccac actaacccgc tcggttgatg cgtgaaatcc
     3781 atgtttaagg agatctttaa tgggttatta aaatctgcca ctggaacact atcgtaagag
     3841 accagattac cgttataaac cacctggtta tcaccggtat tgctctcctc ataaccattg
     3901 tttaacgaca agttgccctt gctcttgata tagctaaaga caatttgtgg gttaatatct
     3961 acttggcgga tatgcagggg ttcggccagt tcaaaactga ggctgaacga atgcgttttg
     4021 gttttgccat cgttgttata ttcagtaatg gtggttttag tcgcgctgtc ggtacgactg
     4081 cttttgctga tttgatcatg cgcttcacgg taaacatagt ttgcacgcgc aataacgttc
     4141 ttaccgattt tctgctgcaa tcccattgcc agttcatcgt tataaggcgt tttcaaatcc
     4201 tgataacgcg tcagagtttt attacctgat accgattccg tccagctatt gcggatatca
     4261 cgtaatccca tatcaagaat attcccgcca tagtaacggt tataaccggc ggtaatcatt
     4321 gaggtttgat cagcaaaaat atcccattcc gtcataaagc gcggggagat attgtggttt
     4381 gacagatagt tgtcatagtc ataacgcaca ccgggcatta acgacacatt ccgccagcta
     4441 atgtgctccg ccatatacag cgtgtagttg tcaattccca ggctgccttt acctttatgg
     4501 taaatggtat ggttagtttt ctttccggca gcattaatca cataggattc agactggtta
     4561 tggcgttcag tccatgcatc ggaatagatg tattccgcgc cgaagtaggg ttgatgcgaa
     4621 acatcaccca cggcgaattt ttgccagtcc aggcgtgttt tgaaggtgta attatctaca
     4681 gcctgggaaa tgtgtcctaa tccgccacag gtacaacgcc ctgtaatatc accatatgta
     4741 catgaaagtt cggtgtacca gatgtcatga tcgtgacggg tataatcact aatatgatcc
     4801 caaccaacgg tggtacgtag tttggcccat gcgagttgcg tatccatatc ccatgccaga
     4861 ccataggatt tattacccat ttcgcgatca gactgcggga aggtgctggt attataatca
     4921 cggctggagc cggtatattt taaggttaaa tcgtgggtaa agcggtcgct ggcaaaccag
     4981 gtaaatttgc tcaatgcagt atcgataacg tttttatact gtgcccgacc ggtgacaatg
     5041 ccgtcattcg aaacataatc cgcgcgggtg atatcagact ggcggcgcga taaaccggcg
     5101 gtaacgccga agttatcagc gagttcctga ttaaacgaca aggtataaaa gttcttttta
     5161 aaatctgggg agtaataagt acttcctgaa gagccttgat taaatgcgct cttgttattc
     5221 tcatcgatat gcgatgttaa ccagtccgaa cgcgtagtgc gataacccaa tttcaccttg
     5281 ctatcatcag cgttgaagcg tttgatcttt gcatcaatta ccccgccatt gaagcgacca
     5341 aattcaaccg gcacaaaact gtcataaagc gtcacattgt ccagtaagct gacatcaaga
     5401 taatacccct gtgacatccc gctaatattg gttgcactac tggcatcgga ctcattcgct
     5461 gggttcaggt tattagttgc actaataccg tcaatcaaat aggcattctg gtagggcgac
     5521 gcaccgtgaa tagagatttt ctcagggcga atatctccct ggttcaacga ggtactttgc
     5581 gttgaatcca tgcgtaccgc agggttggtt ctcagcagat cgctgatatt gccattcccg
     5641 gtcggtaaac gctcgatgct ttcactggtg taatgcgtgt tgccgtttac gggtacaggg
     5701 acaggagcat agaccgtcat ctcttcaaaa aaatgcatgt acatgttttt gtcatcgacg
     5761 gcctgcgcca catcagcgcc acataaaatg acgccaggaa taagaactcg cttcatttat
     5821 ttatatctcc gtacagc
//
LOCUS       DAJVQG010000196         5640 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJVQG010000196 DAJVQG010000000
VERSION     DAJVQG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.196"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="OQM83_003681"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="OQM83_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCS1521832.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="OQM83_003682"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="OQM83_003682"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCS1521833.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="OQM83_003683"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="OQM83_003683"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCS1521834.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="OQM83_003684"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="OQM83_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCS1521835.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1401 a         1351 c         1529 g         1359 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgtaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtattatgca
     5581 caccccgcgc cgctgagaaa aagcgaagcg gcactgctct ttaacaattt atcagacaat
//
LOCUS       DAJVQG010000197         5617 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJVQG010000197 DAJVQG010000000
VERSION     DAJVQG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.197"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            13..429
                     /gene="soxR"
                     /locus_tag="OQM83_003685"
     CDS             13..429
                     /gene="soxR"
                     /locus_tag="OQM83_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCS1521836.1"
                     /translation="MAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYVAII
                     KIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDELDG
                     CIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            975..2324
                     /gene="ghxP"
                     /locus_tag="OQM83_003686"
                     /gene_synonym="yjcD"
     CDS             975..2324
                     /gene="ghxP"
                     /locus_tag="OQM83_003686"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCS1521837.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2476..4125
                     /locus_tag="OQM83_003687"
     CDS             2476..4125
                     /locus_tag="OQM83_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCS1521838.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4279..5570)
                     /locus_tag="OQM83_003688"
                     /pseudo
     CDS             complement(4279..5570)
                     /locus_tag="OQM83_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         1258 a         1270 c         1456 g         1633 t
ORIGIN      
        1 acccccggcg aagtggcgaa acgcagcggt gtggcggtat cggcgctgca tttctatgaa
       61 agtaaagggt tgattaccag tatccgtaac agcggcaatc agcggcgata taaacgtgat
      121 gtgttgcgat atgttgcaat tatcaaaatt gctcagcgta ttggcattcc gctggcgacc
      181 attggtgaag cgtttggcgt gttgcccgaa gggcatacgt taagtgcgaa agagtggaaa
      241 cagctttcgt cccaatggcg agaagagttg gatcggcgca ttcatacctt agtggcgctg
      301 cgtgacgaac tggacggatg tattggttgt ggctgccttt cgcgcagtga ttgcccgttg
      361 cgtaacccgg gcgaccgctt aggagaagaa ggtaccggcg cacgcttgct ggaagatgaa
      421 caaaactaaa gcgccacaag ggcgctttag tttgttttcc ggtctttgtc tttctctcta
      481 tcccgctggt acacaggagg gtttcccccg acgtcaacac accccattcg agcacgtggt
      541 ggaggttccg gttggtgttg atgctttaat tgtatgtcac cggcatttct tcgccagtgt
      601 aaaagtatac tttttatccg caatattttt gtcatctcag acgatttttt atcgcaatcc
      661 tgaacggtat acggctcgat aacgctgcaa tcttgcgcac cgacgataac gtttgcgcat
      721 caattgcctg gtttttcatc gtcaagacaa taaaagagaa aaaagcagca aacttcggtt
      781 gaaaaagccg ctatgatcgc cggataatcg tttgcttttt ttaccacccg ttttgtatgc
      841 gcggagctaa acgtttgctt ttttgcgacg cagtaaattg tcgcaaacct ggagcaggaa
      901 gataacgttt ctctggcagg ggattgtccg ccacgcatct tgacgaaaat taaactctca
      961 ggggatgttt tcttatgtct acgccatcag cgcgtaccgg cggttcactc gacgcctggt
     1021 ttaaaatttc acaacgtgga agcactgtcc gtcaggaagt ggttgccggg ttaacaacgt
     1081 ttctggcgat ggtctactcg gtcatcgtcg ttccaggtat gttgggtaaa gcaggcttcc
     1141 cgcctgcggc agttttcgtt gcaacctgtc tggttgccgg actcggttct atcgtgatgg
     1201 gtctgtgggc taatctgccg ttggcgattg gttgcgccat ctccctgacg gcgtttaccg
     1261 cattcagcct ggtgctgggg caacatatta gcgtacctgt cgcgctgggt gccgtgttcc
     1321 tgatgggtgt gctgtttacg gtaatttctg ccacgggtat ccgtagctgg attttgcgca
     1381 acttgccgca cggtgtggcg cacggcacgg ggatcggtat cggtctgttc ctgctgctca
     1441 ttgccgctaa tggtgtcggt ctggtgatta aaaacccgct tgatggtctg cccgttgcgc
     1501 tgggtgattt cgcgaccttc ccggtgatta tgtcactggt aggtctggcg gtgatcatcg
     1561 gcctggaaaa actgaaagtc cctggtggca ttctgctgac cattatcggt atctcaattg
     1621 tcggtttgat cttcgatcct aacgtccatt tctccggcgt tttcgccatg ccttcattga
     1681 gcgatgaaaa cggcaattca ctgattggca gcctggacat tatgggcgcg ctgaatcctg
     1741 tagtcctgcc aagcgttctg gcgctggtga tgacggcagt atttgatgcc accggaacta
     1801 tccgtgccgt cgccggccag gcgaacctgc tggataaaga tgggcagatc atcgacggtg
     1861 ggaaagcact gaccactgac tccatgagca gcgttttctc tggcctggtg ggtgctgctc
     1921 cggcagcggt atacatcgag tctgcggcgg gtacggcggc gggcggtaaa accggtttga
     1981 cggctatcac cgttggcgtg ctgttcctgc tgattctgtt cctctctccg ctctcttacc
     2041 tcgttccggg gtatgcaacg gctccggcgc tgatgtacgt tggcctgctg atgctgagca
     2101 acgtggcgaa aatcgacttt gctgattttg ttgatgcgat ggcgggtctg gttacggcgg
     2161 tattcatcgt gctgacctgt aacatcgtaa caggcatcat gatcggcttc gcgactctgg
     2221 tgattggtcg tctggtttcc ggcgaatggc gcaagttgaa catcggtacg gtcgttatcg
     2281 ccgtggcgct ggtgaccttc tatgcgggtg gctgggctat ctaatctttc atcggattct
     2341 gaaaacgggt ggcaatggct gcccgttttt attttctccg cacattgtgt gagcttttgc
     2401 gatattctga aaaaatgaga attcaggcat aacgcctttc tcagatcata aaaaaacatc
     2461 gcaaacaggg aacgcatgga aatcttcttc accatactga taatgaccct cgtggtctca
     2521 ctgtccgggg tggtcactcg tgtcatgccc tttcagatcc cgcttccgct tatgcaaatc
     2581 gccatcggtg cgctactggc gtggccgacg tttggtttgc atgtggagtt tgatcctgaa
     2641 ctctttttag tcttgtttat cccgccgttg ctgttcgctg atggctggaa aacgccgacc
     2701 cgtgaatttc tcgaacatgg tagagagatt ttcggcctcg cgctggcgct ggtggtggtc
     2761 accgtggtcg gcattggctt ccttatttac tgggtggtgc cgggcattcc gctgatcccc
     2821 gcctttgcgc tggcggcggt actttctccg accgatgctg tggcgctctc cgggattgtt
     2881 ggcgaagggc gcatcccgaa aaaaatcatg ggcattttgc agggcgaagc gttgatgaat
     2941 gacgcctccg gcctggtgtc gttgaagttt gccgtggcag tggcgatggg gacgatgatc
     3001 ttcaccgtcg gcggtgcgac ggtcgaattt atgaaagtag ccattggtgg tattcttgcc
     3061 ggttttgtgg tgagctggct gtatggtcgc tcgctgcgat tcctcagccg ctggggcggt
     3121 gatgaacccg cgacgcagat cgttctgctg ttcttgctgc cattcgcttc ttatctgatt
     3181 gccgaacata ttggcgtttc cggcatcctc gctgcggttg ccgccgggat gaccatcacc
     3241 cgctccggtg tgatgcgccg tgcgccgctg gcaatgcgcc tgcgcgcaaa cagcacctgg
     3301 gcgatgctgg aatttgtctt taacggcatg gtgttcctgc tgttaggtct gcagctgccg
     3361 ggtattctgg agacgtcgct gatggcggca gaaatcgacc ctaacgtcga aatctggatg
     3421 ctgtttaccg atattattct gatatatgcg gcgctgatgc tggtccgttt cggctggctg
     3481 tggacgatga aaaagttcag caaccgcttc ctgaagaaga agccgatgga gtttggttcg
     3541 tggaccacac gagaaatcct gatcgcgtct ttcgccgggg tgcgtggggc gatcactctg
     3601 gccggtgtgc tctctatccc gctgctcttg ccggatggta acgtcttccc ggcgcgctat
     3661 gagctggtgt tcctggcggc tggcgtcatt ctcttctcgc tgtttgtcgg cgtggtgatg
     3721 ttgcctattc tgctacaaca cattgaagtc gcggaccatt cgcaacaatt gaaagaagaa
     3781 cgtattgcgc gagcggcaac ggcagaagtg gcgattgtgg cgatccagaa aatggaggag
     3841 cgtctggcgg cggataccga agagaatatc gataaccagc tgcttacgga ggtcagttct
     3901 cgcgtcattg gtaacctgcg tcgtcgcgcc gatggacgta acgacgttga aagttccgtg
     3961 caggaagaga accttgagcg tcgcttccgt ctggcggcat tgcgttctga acgtgctgaa
     4021 ctgtaccacc tgcgcgccac gcgggagatc agcaacgaaa cgctgcaaaa attactgcac
     4081 gatctcgatt tgcttgaagc gttgctaatt gaggaaaatc agtaagccga aagcgacgta
     4141 accaggcccg aggtaatctg aaaaaggtgc aatcttttgc acctttaaca ggttagctcg
     4201 ggctaacatt catgattcta aaacaaataa gttatctctc ttgtaaagtc aattcattgg
     4261 ctgttcttcg acttaacctc aatctgcgtg ttgcataata tctgcagtaa tcttcatagc
     4321 gactgcatta atattatcct cagtttgtga catgccagta tgagaggtac aatcttcttc
     4381 aaagaatttc tgtagtgtta ccagggtatc actatcaatg gggatatttt cctccccacg
     4441 gagtatggaa atgcccattc cctggacgct agcctcatca aaattaaccc gatagagacg
     4501 ggtattaaac atacaggctt ttgttaaaat agaacagttg aaatggcagt tgcttaaatt
     4561 ggcattatta aaattaacat tagccatatt agagtcggta catataacca tttcgaggaa
     4621 tgtatttgtt agccacgtac aaaagagttt gctatcaatt aagtttatat tctggaggat
     4681 tgctttgttc agattgacgc gagacagatc agcatgttca aagttaatta aatcgagttt
     4741 agagaaacta aggtcggctt taaataaatt aacatatggc gctattactt tataaagatt
     4801 tgcctcagcc atgtacgcca tataaaaatt tgaatagtca agaaaggtat ttttaatatt
     4861 ggctctattc attgctgcat tgtagaattg aacatgcata acggagcatg catttagagt
     4921 tccgtcggcc atttttatct gagcgaaatt tgagtcatcg agaatacaat tatcaaaaag
     4981 tatttttcca ttatggcgta ataaaaattc gttttataca tattacagtc ctggaaaata
     5041 gcgttttgta gtctgcaacc tgcaaaattc gaattggata aatcttcatc tttaatcacg
     5101 atacctttca ggctaaggtg tgaaaaatcg taaccaccat ttactgtctg tggaatttcg
     5161 atagtattat tatggacggc gacattattt ataaaatata ccaccttaat ggatacttcc
     5221 ttttctttac taaggtaaac tcttctcgta attctatcgt cacatttatt atttgcatat
     5281 aaagctatat ctataaaatg tttatgtatt ttcctgtccg tttccttcgt gatttttcct
     5341 gatggtaaac tatcataaat tcttttcatt ttctgaatca aacctatcac tttttcatga
     5401 tcatccatct tgaaaagaga gagggcttta ttgtcaatta acgtgttttt atgatgtttg
     5461 ctgtaattat atgattttgt tttctttgta tgtgatgaac ttgtgaaaga gtggttatcg
     5521 ttaatctggc aaagagagaa aaacatatta tttaaaccat tatagcgcat aaaatatcct
     5581 tataattaac aagaaagaaa aggcatattc tctgcat
//
LOCUS       DAJVQG010000198         5616 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJVQG010000198 DAJVQG010000000
VERSION     DAJVQG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.198"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..513)
                     /locus_tag="OQM83_003689"
     CDS             complement(<1..513)
                     /locus_tag="OQM83_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCS1521839.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(759..1211)
                     /locus_tag="OQM83_003690"
     CDS             complement(759..1211)
                     /locus_tag="OQM83_003690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1521840.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="OQM83_003691"
     CDS             complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="OQM83_003691"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCS1521841.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="OQM83_003692"
     CDS             complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="OQM83_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCS1521842.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3091..4803
                     /locus_tag="OQM83_003693"
                     /pseudo
     CDS             3091..4803
                     /locus_tag="OQM83_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="OQM83_003694"
     CDS             complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="OQM83_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCS1521843.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5494..>5616)
                     /locus_tag="OQM83_003695"
     CDS             complement(5494..>5616)
                     /locus_tag="OQM83_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1521844.1"
                     /translation="IAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLEGSGLS"
BASE COUNT         1339 a         1467 c         1458 g         1352 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcca gtgcaaacag
       61 ttcagcgtcg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcgcc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccaaagc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccatg ccgcagccga tatcattgcc
      301 gaccagtgct gggtaaaaac ggccaacaga gaagaacgct gcgccaatcg ggtagccgcg
      361 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
      421 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
      481 cgcatcggat aagggacgaa tatatttgcc catgtaccat tccgtaaata gactcgtcat
      541 acttcaagtt gcatgtgctg cgactcgaat tattgagagt aacaattaaa aaatcaggaa
      601 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
      661 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
      721 acatactagg cgggcgttat tttcattgca agctggattt aatgttgcgg tttatattgc
      781 atataaaaat tagtaaacca cgctccccgg cattcaacgt gctgctgctt aactgtctga
      841 aaaccataac gttcaaaaaa gggttttgcg gttatgctgg catctaccgt aagttcggat
      901 tcagacttaa taaatggttt taacaaggcg ctggcaaccc cgcggcgggt gtattcaggg
      961 tcaacaaata acatatcgat ataatgttca atgcgggaaa taaaaccaac cggttgtgca
     1021 ttaatgaccg caacccacac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     1081 atctgcgccc aggcggcaat ttgttgtgga gaataatgct gactggcggt catcgtaacc
     1141 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtt tcttatttgt
     1201 atgttattca taatcccagc accagttgtc tttccatttg cggatcaagc aacgtcacat
     1261 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     1321 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     1381 aatcgtcgaa ctttaatttc accccctggc gggcaatcag taaatcaggt tttacctttg
     1441 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     1501 ggtggatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     1561 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     1621 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtt cgtagcccca
     1681 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agtggtaagg
     1741 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctggccgttg ggtttattca
     1801 tgtcggaggc gattttggcg agaaacttta cgggtgccac gcccgcagac gccgtcagtt
     1861 gcagctcgtt gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     1921 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     1981 aggtgtagcg cgagaagatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     2041 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     2101 tacgtacgcc aaatttacgc gcgggataat tggcggtact gatcaccccc cgacgttcgc
     2161 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     2221 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     2281 tggtaaagta tacagtgatt tcagagtttg agaaatgcgt aaagattcag catttaacgc
     2341 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     2401 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     2461 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     2521 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     2581 cgagccgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     2641 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     2701 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     2761 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     2821 tccagattcg cttccatatg cgcatcacgg gcaatggtgg tgatactggt tgccagctcg
     2881 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattgac gtcgcgcgct
     2941 tttttctccg tttcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     3001 attttattta acggcgacag cccgccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     3061 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat tgtcgtcacc
     3121 aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc gatgcccggt
     3181 aagcagatgg cgattgacgc cgatcttaat gccgggttga tcaatcaggc gcaggcacaa
     3241 acccgacgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
     3301 aaatttgtgc gcggggatgc tatcgccggg atgatgattc tggcgatcaa cctgatcggc
     3361 ggcgtctgta tcgggatctt caaatacaac ctgagcgccg acgccgcctt ccagcagtat
     3421 gtgctgatga ccatcggcga cgggctggtg gcgcaaatcc cttccctgct actctccacc
     3481 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgt
     3541 aaccagttgc tggcaagccc atcggtgctc tacaccgcta ccggaattat gttcgtactg
     3601 gcggtagtgc cgggaatgcc acatctgccg tttttgctgt tcagcgccct gcttggcttt
     3661 accggctggc ggatgagcaa acgcccgcag gcggcggaag cggaagagaa aagtctcgaa
     3721 acgctgaccc gcaccatcac cgaaaccagc gagcaacagg tcagttggga aaccattccg
     3781 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcc
     3841 cagggcaacc cgctcaccca gcgtattcgc ggcgtgcgac aggtgatatc cgacggcaac
     3901 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gccttaagcc cagccagtac
     3961 gctattttca tcaacggcat taaggctgat gaagcggata ttccggcgga taaactgatg
     4021 gcgctgccct ccagcgaaac ctacggcgag atagacggcg tgctggggaa cgacccggcg
     4081 tacgggatgc cggtgacctg gatccagcct gcgcagaaag cgaaggcgct gaatatgggg
     4141 tatcaggtga tcgacagcgc cagcgtgatt gctacccatg taaataagat tgtgcgcagc
     4201 tatattcctg atttatttaa ctatgacgat attacgcagt tgcataaccg tttgtcgtcg
     4261 atggcaccgc gcctggcgga ggatttaagc gcggtgctca attacagcca gttgctgaaa
     4321 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
     4381 gtgctggtcg ccagtagcgc ggtgaccaaa gatcatattc tgctggcagc cgatgtgcgc
     4441 ctggcgctgc ggcgcagcat tacccatccg ttcgttcgca agcaggagct gacggtgtat
     4501 accctgaata atgagctgga aaatctgctg actaacctag tgaatcaggc gcaacagggc
     4561 ggtaaagtga tgctcgacag cgtgccagtg gacccgaata tgctcaacca gttccagagc
     4621 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagacccggt gctgctggtg
     4681 ccaccgcagc tgcgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
     4741 gtgctgtcgt ataacgaagt gccggacgag ctggagttga agattatggg ggcgttgatg
     4801 taagtatccg gcattggtgc acaactgccg gatgcggcgt aaacgcctta tccggcctac
     4861 cgttcaggca caagtttgta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
     4921 cataattata aaatgatacg ctcgccagca tttatatccg ttacgtcccc cgcccaatcg
     4981 atgggatata accctcgcgc gacatcgcga tggaacgttg agaatggcca atcactcact
     5041 tgctttaccc aaccatgctt tactggattt atataaatat aatcaacatg atgcctataa
     5101 tctttggtat tgcggatggc gtgctcccaa aaacgtggtt gccagatatt tttcaatcca
     5161 caagcatggg taaattgctt tttaatttcc cgccagcgag aggaaaaatc atcatcgcct
     5221 tcaggtaatg tccatatgca atgcatatgc tctggcaaaa cgacccaggc gttgatttca
     5281 aaaggcctgt ctcgcttaac tttaataatg gcgttacgga gcatctggta ctgggtggtc
     5341 aagagttggc ttcgacgatt gcgtaaattc accgtgaaaa accaggttcc ccccttgatg
     5401 taataacgac gatattcaga catggcgact cctttttctc aacggtaaga agaaaaggaa
     5461 aggtcggcaa tgaggtgatg gaacagggca agattacgaa agcccgctcc cctcaaggac
     5521 tgacgccaga tagtttctgt ccatggctgc ttttcgcatc ttacgtctta accctgcctt
     5581 gaatacctta tcattcgtca aaatattaat agcgat
//
LOCUS       DAJVQG010000199         5586 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJVQG010000199 DAJVQG010000000
VERSION     DAJVQG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.199"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            547..894
                     /locus_tag="OQM83_003696"
     CDS             547..894
                     /locus_tag="OQM83_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCS1521845.1"
                     /translation="MILDSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFI
                     GSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEI
                     GAVPAIPQQSSLF"
     gene            947..1342
                     /locus_tag="OQM83_003697"
     CDS             947..1342
                     /locus_tag="OQM83_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCS1521846.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            1383..2126
                     /gene="cmoA"
                     /locus_tag="OQM83_003698"
     CDS             1383..2126
                     /gene="cmoA"
                     /locus_tag="OQM83_003698"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCS1521847.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            2123..3094
                     /gene="cmoB"
                     /locus_tag="OQM83_003699"
     CDS             2123..3094
                     /gene="cmoB"
                     /locus_tag="OQM83_003699"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCS1521848.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(3259..>5586)
                     /gene="torZ"
                     /locus_tag="OQM83_003700"
     CDS             complement(3259..>5586)
                     /gene="torZ"
                     /locus_tag="OQM83_003700"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCS1521849.1"
                     /translation="DGKILTAERWGAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQ
                     VHTTTRIQHPMVRKSYLDNPLQPTKGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPS
                     AIFAGSYGWRSSGVLHKAQTLLQRYMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVY
                     EQQTSWPLILENSQVVVLWGMNPLNTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPI
                     RSETIDFFGDNATWIAPNMGTDVALMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYL
                     RGKSDNTPKSAAWAAEITGVPEAQIVKLAELMAANRTMLMAGWGIQRQQYGEQKHWML
                     VTLAAMLGQIGTPGGGFGFSYHYSNGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRA
                     IPVARIVDALENPGGKYQHNGKEQTYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEM
                     IVVSECYWTAAAKHADIVLPITTSFERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDF
                     DVFADLAELLKPGGKEIYTEGKDEMAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKL
                     IEMRRSEKNEQYVRYGDFRADPVKNALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAP
                     DEWKGTADEKQLQLLTAHPAHRLHSQLNYAELRKKYAVADREPITIHTEDAARFSIAN
                     GDLVRVWNKRGQILTGAVVTDGIKKGVVCVHEGAWPDLENGLCKNGSANVLTADIPSS
                     QLANACAGNSALVYIEKYTGNAPKLTAFDKPAVQA"
BASE COUNT         1291 a         1425 c         1341 g         1529 t
ORIGIN      
        1 ttagctgtct cataatccaa aaacaacctt ttgagactac ttgagggtgc attctaccgt
       61 tacgttgaaa gtgtctcaaa aggacttcct tatgagactg gttaatagag cgctatgaag
      121 attcaggagg gtaactataa ccatcacttt ctaactttca cgtaggaaat cgttaagttt
      181 cttgatagtt acaccatgtg accaactatc tatatatcgt tatatagccc tgtatatagc
      241 actatatgta gtgtctggag agggacgact aacacgatac agtttaccct gaaaagagac
      301 ttcacagatt ctttatatat ttcaataagt tatgctaaaa agagactata ccgatactgc
      361 atagaatttg tgtaggattg catagatact tggaaaaaca ccgttaaatt gcatagtcat
      421 tcacctgata ttgtcctgtt tttacaggta gttgattcgg atgttctagg aatcatcctc
      481 atagttgatt cggttagttg ggcttgtacc aacccctaat caacctcata gtgtatttag
      541 accttgatga ttttagacag ccgccctgtt catgcagcac gtccacacag tgaagctatt
      601 cgcgacgctc aacgaaaaaa acctaaagtt ccggtacatg ctgtactgac ggcgacaaat
      661 ccgctgattc gttttatcgg tagtgatgat atgacgcaaa accgggaatt atttcaggtc
      721 tggttacaga aattagcgca gtggcatcag accactacgc cttatctttt tttacatacg
      781 ccagacattg cccaggcccc ggaactggta cataccctgt gggaagactt acgtaaaacg
      841 cttccagaga tcggagcagt tccggctatt ccacagcaat cttctctttt ctgaatttgc
      901 cacctatcat agacaggtgc catcggccat tttaaaggga gtttgtatgg taagcgcgct
      961 atatgccgtt ttaagtgcgt tgttattaat gaagttctct tttgatgtcg ttcgcctgcg
     1021 aatgcagtac cgcgttgcct atggtgacgg tggttttagc gaactgcaaa gcgctattcg
     1081 cattcatggt aacgcggtgg aatatattcc catcgcgatt gtgctgatgc tgtttatgga
     1141 aatgaatggc gcagaaacct ggatggtgca tatttgcggc atcgttttgc ttgctggtcg
     1201 tctgatgcat tattacggtt ttcatcaccg tctgtttcgc tggcgacgtt ccggcatgag
     1261 cgccacctgg tgtgcgctgt tgctgatggt gctggcgaat ctttggtata tgccctggga
     1321 gttggttttc tccctgcgtt agcgcacaat acgccacttt ctttttcccg gatttttacg
     1381 ttatgtctca ccgcgacacg ctattttctg cccctatcgc cagactgggc gactggacct
     1441 ttgatgaacg ggtagctgaa gtcttcccgg atatgatcca gcgttccgtt cccggctatt
     1501 ccaatattat ttccatgatt ggtatgttag ccgagcgctt cgttcaacct ggtacgcagg
     1561 tttacgatct gggttgttct ctgggcgcgg cgacgctctc ggtgcgtcgc aacattcatc
     1621 atgataattg caaaattatt gccatcgaca actccccggc gatgattgaa cgctgcagtc
     1681 gtcatattga cgcctataaa gcccctacgc cagtagacgt cattgaaggt gatattcgcg
     1741 atatcgccat tgaaaacgca tcgatggtgg tgctgaattt taccctgcaa ttcctggaac
     1801 cttccgagcg ccaggcatta ctggataaaa tttatcaagg actgaacccg ggcggcgcgc
     1861 tggtgctttc ggaaaaattc agtttcgaag atgccaaagt tggtgaactg ttgttcaaca
     1921 tgcaccacga ctttaaacgt gccaacggtt acagcgaact ggagatcagc cagaagcgca
     1981 gcatgctgga aaacgtgatg ctgaccgatt ccgtggaaac ccataaagca cgcctgcata
     2041 aagccggttt tgagcatagc gagctgtggt tccagtgctt taactttggt tcactggtgg
     2101 cattaaaagc agaggacgct gcatgatcga ctttggtaac ttttattctc tgattgccaa
     2161 aaatcatctt tcacactggc tcgaaacgct gcccgcgcag attgctaact ggcagcgcga
     2221 gcagcagcac gggctgttta agcagtggtc caacgcggtg gaatttctgc ctgaaattaa
     2281 accgtatcgt ctggatttat tgcatagcgt aaccgccgaa agcgaagagc cactgagcac
     2341 cgggcaaatt aaacgcattg aaacgcttat gcggaacctg atgccatggc gcaaagggcc
     2401 gttctcactg tatggcgtca acatcgatac cgaatggcgt tccgactgga aatgggatcg
     2461 tgttctgccc catctttctg atttaaccgg acgcaccatt ctggatgtcg gctgcggcag
     2521 cggttatcac atgtggcgca tgattggcgc aggcgcgcac ctcgcggtgg ggatcgaccc
     2581 tacgcaactg tttctgtgcc agtttgaagc ggtgcgtaaa ctactgggta acgatcagcg
     2641 cgcacatttg ttaccgttag gtattgaaca gcttccggca ctgaaagcct ttgataccgt
     2701 cttttcgatg ggcgtgcttt accatcgccg ttcgccgctg gagcatctct ggcagttgaa
     2761 agatcaactg gtgaatgagg gtgaactggt gctggaaacg ctggttattg atggcgatga
     2821 gaacacggtg ctggtaccag gcgatcgtta cgcgcaaatg cgtaatgtct atttcatccc
     2881 ttccgcgctg gcgctgaaaa actggctgaa gaagtgtggt tttgtagata tccgcattgt
     2941 ggatgtgtgc gttaccacta ctgaagagca gcgacgcact gaatggatgg ttaccgaatc
     3001 tctgtccgat ttcctcgacc cacatgatcc gagtaagaca gttgaaggtt atcctgcgcc
     3061 taaacgcgcg gtgctgattg cgcgcaagcc gtaaaggtct ggtattactg ccggatgcgg
     3121 cgtgaatgcc ttatccggcc tacaaaccct tgctaattca atctattgca gggactatgt
     3181 aggcctgata agcatagcgc atcaggcagt tttacgtttg cataacctca gcgcccgttt
     3241 ccgggcgcta ttcacttctt acgcctgaac agcaggctta tcaaatgccg ttaacttcgg
     3301 tgcgttaccc gtatattttt cgatatacac cagcgcagag ttaccggcac aggcatttgc
     3361 cagctgcgag ctggggatat ccgccgttaa cacgttcgca ctgccgtttt tacacaagcc
     3421 gttttccaga tctggccatg caccttcatg cacgcatacc acgccttttt tgatcccgtc
     3481 agtcaccacc gcgcctgtca aaatctgacc acgtttgttc cagacacgca ccagatcgcc
     3541 attcgcaata ctaaatcgag cagcatcttc ggtgtgaata gtgatcggtt cacgatcggc
     3601 aaccgcatat tttttacgca gttccgcata gttaagctga ctgtgtaaac ggtgtgccgg
     3661 atgtgcggtc agaagctgca actgcttctc gtcggcggta cccttccact catcaggcgc
     3721 aagccaggtt gggtgtgccg ggcaatcctt atagccaaat ttttccagcg ttttggagta
     3781 aatctcaatt ttgccacttg gcgtacccag cgcatttttc accggatcgg cgcggaaatc
     3841 accataacga acgtactgtt cgttcttctc gctgcggcgc atttcgatca gtttattttg
     3901 ctgccagaag gcattaaaca ttggcatagt gacgcgttgc gcacgggccc ctttctgagc
     3961 ggcatcatag aaaaatttca gccacgccat ttcatcttta ccttcggtat agatctcttt
     4021 tccgccaggt ttgagtaatt ccgcaagatc ggcaaacaca tcaaaatcgt ttcgcgcttc
     4081 aaattgcgga gcaacagcct gcttcatcgg cacaatatgc tggttgctgt aatcaccggt
     4141 catcgtcaag tcattgcgct caaacgatgt ggtgatcggt aatacgatat ctgcgtgttt
     4201 agcagctgcg gtccagtagc attcagaaac gacgatcatc tccggtttct gccatgcttt
     4261 aatcagacga ttagtatcct ggtgatgggt aaagttaccg ccacccgccc accagatcat
     4321 tttgatattc ggatacgttt gctctttacc gttatgctga tatttgccgc caggattttc
     4381 cagcgcatcg acgatacggg caacaggaat cgccctcatt ccgccatcat cagcggcctc
     4441 gctggcctgt ccggcaatcg ccgcagacat ttccggcagc acgccgccaa cacgtgtcgg
     4501 gttgccacca ttggagtaat gataagagaa accaaaaccg ccgcccggtg taccaatctg
     4561 ccctaacatc gccgccagcg tcaccagcat ccagtgtttc tgttcaccat attgctggcg
     4621 ctgaattccc cagcctgcca tcagcattgt gcgattcgcc gccatcagtt cagcgagttt
     4681 gactatctgc gcttccggta cgccagtaat ttccgccgcc caggccgcgc tcttcggcgt
     4741 attatcgctc ttacctctca gatactcttc aaactgcgga tacccggtag tgtatttttc
     4801 aaggaacact ttatcgtgtt tgccttgtgt catcagggta tgcgcaatcc ctaacatcag
     4861 tgccacgtcg gtgcccatat tcggcgcgat ccaggtggca ttatcgccaa agaagtcgat
     4921 ggtttcggag cggataggat caatggcaat cactggtttg ccagattttt tcagctgatg
     4981 gaagtattcc agcccttgct catcggtact gctccaggca atttttaagg tattcagcgg
     5041 gttcatcccc cacagcacca caacctggct gttttccaga atcagcggcc aggaggtctg
     5101 ctgttcatac acctctacag aaccgaccac atgcggcatg atcacctgtg ccgccccggt
     5161 tgaataatcg ccgctatgcc cggaataacc gcccgccagg ttcatataac gttgcagtaa
     5221 ggtttgcgct ttatgcaaca cgccagaaga gcgccagccg tatgatccgg caaaaatagc
     5281 cgatggtccg ttagctttgc gaatgcgatc gtgttgctca tgaatcagtt ttaatgcttg
     5341 ttcccagctc acctgtacat aggtatcttc gccacgacct ttcgtcggtt gcagtggatt
     5401 atcgagatag ctttttctca ccatcggatg ctgaatacgc gtcgtggtgt gtacctgatc
     5461 cgccgccgta gactgtaagg aattcggtat ggttttcgcc agcgcgcctg ttgaagaaac
     5521 aatcttgccg tccttcactt ctacgttcat cgctccccaa cgttccgcgg tgaggatttt
     5581 accgtc
//
LOCUS       DAJVQG010000200         5520 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJVQG010000200 DAJVQG010000000
VERSION     DAJVQG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.200"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..171
                     /locus_tag="OQM83_003701"
     CDS             <1..171
                     /locus_tag="OQM83_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1521850.1"
                     /translation="WRANGTPPSFHEQRSLSERLFREQGVDTKILLGHSNQKMTDIYN
                     DVRGKEWKKLVI"
     gene            complement(204..842)
                     /gene="ompW"
                     /locus_tag="OQM83_003702"
     CDS             complement(204..842)
                     /gene="ompW"
                     /locus_tag="OQM83_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCS1521851.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1082..1171
                     /locus_tag="OQM83_003703"
                     /pseudo
     CDS             1082..1171
                     /locus_tag="OQM83_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1199..1942
                     /locus_tag="OQM83_003704"
     CDS             1199..1942
                     /locus_tag="OQM83_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCS1521852.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            1972..2511
                     /locus_tag="OQM83_003705"
     CDS             1972..2511
                     /locus_tag="OQM83_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCS1521853.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            2616..3014
                     /gene="yciA"
                     /locus_tag="OQM83_003706"
     CDS             2616..3014
                     /gene="yciA"
                     /locus_tag="OQM83_003706"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCS1521854.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3054..3773)
                     /gene="tonB"
                     /locus_tag="OQM83_003707"
     CDS             complement(3054..3773)
                     /gene="tonB"
                     /locus_tag="OQM83_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCS1521855.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            3864..5384
                     /locus_tag="OQM83_003708"
     CDS             3864..5384
                     /locus_tag="OQM83_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1521856.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
BASE COUNT         1410 a         1286 c         1327 g         1497 t
ORIGIN      
        1 tggcgtgcta atggcacccc accctctttc catgagcaga gatctttatc agagcgattg
       61 ttcagagagc agggggttga taccaaaatt ttgctaggcc attcgaatca aaaaatgacc
      121 gatatttaca acgatgtacg cggtaaggag tggaaaaaac tggtcatttg ataaccagtt
      181 ttgcagaggt tttgcagaga aaattaaaag cggtacccgg cagagaacat aaacacccac
      241 ggatccagtc gtaccgagtc ttttacggta gtaacaccag atttgtactt agccgtggta
      301 tcgatatcca tgtaccacac tgacatgtta accaaccagt cacggttaat cagataatca
      361 acccccacct gcccggcagc tccccagtaa tctttcagac tgagatcgga aagccccgcc
      421 tctttgccat gatcgttaaa tccattatca aagaaggtgg tgtagttaat acctgcccca
      481 acataaggac ggaatttgct gccggcatca ccaaaatacc actgcgccat cagtgttggc
      541 ggcagatgat gaacggttgc aatatcgccg gtcgcccggg tgccgatttt atggcggaac
      601 ggcgtcgctg ccagtaattc cacaccaatg ttgtcggtcg ccatataagt aaacgtcagg
      661 cccagttgcg tgttattggt cacgctgaat ccacccagac ttcctaacgt accaccagca
      721 ccttctgttg gacgtacggt tgcagaacct gcacgcataa aaaattcgcc tgcttcatgc
      781 gcaaaggcac tgccagagag aagagttgtt actgccaaag ccgccactgt taactttttc
      841 atatccgctc cgtcgttatg gttataaaaa cgtgacgaat atacctacat tggagtaaca
      901 aatgatccta catagatcac attacatatg ataattcacc ctttattgat ctggattaat
      961 ttagaagttg cattgagaat cctggttaac gtgatttaaa tcaattttta gatattttcg
     1021 ctacgcaaat attaactcgt tcgccacttg ccgcggtttg cccacgcctg aaggccagca
     1081 attacctccg ctttacaaag atatgctttt ccatacaaga cctgatgctg ccacccgagg
     1141 cttaaccagg gtacaattgc ccgctaatta acacctgcat aaaactcaag gagagtgcat
     1201 gtctatcacg gcgcagtccg tataccgtga caccggaaat ttcttccgta atcaatttat
     1261 gaccattctg ttggtatcgt tgctatgtgc gtttatcaca gtggtgttag ggcatgtttt
     1321 ctcacccagt gatgcacagc ttgcgcagct caatgacggc gtgcccgtta gcggcagtag
     1381 tgggttgttc gacctggttc agaatatgtc accggaacaa caacaaattt tgctgcaggc
     1441 ttcagcggcg tccacttttt caggattaat cggtaacgcc attctcgccg gaggcgtaat
     1501 attaattatc cagctggtgt ctgcgggtca gagagtcagt gcgctgcgag ctattggtgc
     1561 cagtgcgccg atattgccca agttatttat tctgattttt ctgactaccc ttttagtaca
     1621 gattggcatc atgctggtgg tcgttccggg aattatcatg gccattttac tggccctggc
     1681 accggtgatg ctggtacagg acaaaatggg catttttgcc tcgatgcgta gcagtatgcg
     1741 gctgacttgg gcgaatatgc gtctggtggc acccgcagta ctgagctggt tgctggcaaa
     1801 aacactgttg ctgctttttg cctcttcttt tgccgcatta accccggaaa ttggtgccgt
     1861 actggcgaac accttgagca acctgatttc agccatattg ctcatctatc tgttccgcct
     1921 gtatatgttg attcgccaat aaccttaagc atttgattac ggaatcgtaa aatgaagcag
     1981 tttcttgatt ttttaccgct ggttgtcttt ttcgcgtttt acaagattta tgacatctat
     2041 gcggctactg cggcgctgat cgtcgccacg gcgattgtgc ttatctatag ctgggttcgc
     2101 tttcgtaagg ttgagaagat ggccctgatc acttttgttc tggtggtcgt cttcggtggc
     2161 ttgacgctgt tcttccacaa tgatgagttt attaaatgga aggttacagt catttatgct
     2221 ctgtttgcgg gagccctgtt agtcagccaa tgggtgatga aaaagccgct aattcagcgg
     2281 atgctgggta aagaactcac gctgccgcaa ccggtatggt cgaagctaaa tctggcctgg
     2341 gctgttttct ttatcctttg cggtctggca aacatctaca tcgcattctg gctgccgcaa
     2401 aatatttggg tcaactttaa agtctttggc ctgaccgccc ttaccttaat cttcacattg
     2461 ttaagcggta tctatatcta ccgccacatg ccgcaggaag ataaatccta accagactgc
     2521 cagtccttcg gggctggcgc tgtatctcct ctgttctcct caggaaaatc atagtagcat
     2581 cgcgcctgtg attttccttt taagtcggtt ttaccatgtc tacaacacat aacgtccctc
     2641 agggcgatct tgttttacgt actttagcca tgcccgccga taccaatgcc aatggtgaca
     2701 tctttggtgg ttggttaatg tcacaaatgg atattggcgg cgctattctg gcaaaagaaa
     2761 ttgcccacgg tcgcgtagtg actgtgcggg ttgaaggaat gactttctta cggccggttg
     2821 cggtcggcga tgtggtgtgc tgctatgcac gctgtgtcca gaaagggacg acatcggtca
     2881 gcattaatat tgaagtgtgg gtgaaaaaag tagcgtctga accaatcggg caacgctata
     2941 aagcgacaga agcattattt aagtatgtcg cggttgatcc tgaaggaaaa cctcgcgcct
     3001 tacctgttga gtaatagtca aaagcctccg gtcggaggct tttgactttc tacttactga
     3061 atttcggtgg tgccgttaat tttaaacagg atattcacca caatcccact gcctggctta
     3121 cccggctcat aacgccatct gcgcatcgca tttttcacct cacgctcaaa catgttcgca
     3181 ggcttggctg agaggatttg tacgttatcc acgcgaccat ccggcgtgac gtcaaattta
     3241 actttaacct gcccttcaat gcgcaatgcc tgtgctcgtg ccggatactg cggctgatta
     3301 cggcttaatg cgcgtggtcc tgaagccaca ctggtaaccg gcttgctggt tgcagccgtt
     3361 gccgtacttg atgtggggcg cgcgggtgcc gtattttcaa acggtgatgc cggacgcgac
     3421 tctacgggtt tgacatcgcg ttttggctgc tcctgtacct ttttcaccgg cttcggtttt
     3481 ggcttaggtt tcggcttcgg cttttcaatg accaccggtg cttcttttgg cggttcgggg
     3541 atcggctcag gttccggttc tggctctacc accggctccg gtggcggctg aacggcttgt
     3601 ggcggttcga gatcagcagg cgcaaccatc gtgacagaaa ttggctgcgc aggcgcaggt
     3661 agttcaataa cctgatgtac cgaggtatag agcagacccg ccacaacagc accatgaatg
     3721 cagaccgaaa gtaacgtcgg ccaggggaag cggcgaggta aatcaagggt cattgtattg
     3781 tagctattcg gcattgtagc attagccata acgtttataa gttcatcttt aaatgataat
     3841 caattgcatg ggagtttatc accgtgagaa ccgtaataac atacttgaga ttctcatcag
     3901 ccatacaagg cgctgaaggt gcagattcaa ccagacgaca aaacgacctg ttcaagcagt
     3961 ggctgaagaa gaatggcgat gctcaaatag tagcgtcttt cagtgatgaa gggttgagtg
     4021 gttacaaagg taaacacctt actggtcagt ttggtgacat gttagcccgt attgaggctg
     4081 gggagtttcc agaaggtaca attctgctag ttgagagtat cgaccgtata ggccgattag
     4141 agcatcttga aacagaagcc ttgatgaacc gcatcttagg caacgggata gagattcaca
     4201 cattgcaaga tggccttatc tacacaaagg atgctttagc cgatgacctt ggcataagca
     4261 tcatccagcg tgtcaaagcc tacatagcgc atcagaaatc taagcagaag tctttccgtg
     4321 tttctcagaa atgggggcaa cgtgcaaaac ttgcccttgc tggtgaacaa cgtcttacaa
     4381 agatggttcc tggatggatt gaccctgaga cttttaaact caatgaacac gctgagactg
     4441 tgagattgat attcaaactg ctgctagatg gtgaaagtct gcataacatt gcacgtcacc
     4501 ttcaaagcaa cggtataaag tcgtttagtc gccgtaaaga tgctaatggg ttctctgttc
     4561 actctgtacg caccattcta aggtcagaga caacaatagg cacgttacca gcatcacaac
     4621 gtaatgaccg ccccgctata ccgaactact acgaaggtgt tgtagatata ccaacgttca
     4681 ataaagctca agagattctc gacaagaatc gtaaaggccg tacacctgca agtgacaacc
     4741 cactaacgat taacatcttc aaaggtctgt ttaggtgtca gtgtggggct agtgtccatc
     4801 ctaccggaac aaagaataag tatgctgggg tctacaggtg caataaccac ttagacggtc
     4861 gctgtgatgt tccaccgttg aagcgtaaac cgtttgaccg atggatgatt gataattttc
     4921 tggggatgat tgacgtgggg aatgatggag aatcagagag aaagattgca gcgttacagc
     4981 atgaggttga aattgtcaca gccagaatca agaaagctac cgccctactt cttgagatgg
     5041 atgatattga tgaactaaaa attcagctta aggaactgaa ccagaagcgc acagaactac
     5101 agaccacgat tgataacatg aggcgtaagg cttcactcac tgataaggaa ctaccccagc
     5161 ttaaagatat tgaccttatg actaaagcgg gtcgtgtcga gtgtcagttg attttgtcca
     5221 agcatctaaa agggcttaca ttgggtaagg attcggtaac tgtaacgcta cagaacgaca
     5281 ctgaaatcac catacctaca aacccgttac cgctaaatga tgggtcacca atctttgaaa
     5341 ttgctgataa agagctactg gatattgacg cttatcaact gtaattttca ggggctatga
     5401 agcccctttt tatgtctcaa taagtgccct tcttgttagg gttcacagag tactttcgca
     5461 gagcattacc cggcttaagc acctttgaag cttgctcatt agcggcatct gagaaggtgc
//
LOCUS       DAJVQG010000201         5421 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJVQG010000201 DAJVQG010000000
VERSION     DAJVQG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.201"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..115)
                     /locus_tag="OQM83_003709"
     CDS             complement(<1..115)
                     /locus_tag="OQM83_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCS1521857.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLE"
     gene            complement(220..1722)
                     /gene="dtpA"
                     /locus_tag="OQM83_003710"
     CDS             complement(220..1722)
                     /gene="dtpA"
                     /locus_tag="OQM83_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCS1521858.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(2333..2968)
                     /gene="nth"
                     /locus_tag="OQM83_003711"
     CDS             complement(2333..2968)
                     /gene="nth"
                     /locus_tag="OQM83_003711"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCS1521859.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(2968..3663)
                     /gene="rsxE"
                     /locus_tag="OQM83_003712"
     CDS             complement(2968..3663)
                     /gene="rsxE"
                     /locus_tag="OQM83_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCS1521860.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(3667..4287)
                     /gene="rsxG"
                     /locus_tag="OQM83_003713"
     CDS             complement(3667..4287)
                     /gene="rsxG"
                     /locus_tag="OQM83_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCS1521861.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(4291..5349)
                     /gene="rsxD"
                     /locus_tag="OQM83_003714"
     CDS             complement(4291..5349)
                     /gene="rsxD"
                     /locus_tag="OQM83_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCS1521862.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         1441 a         1373 c         1329 g         1278 t
ORIGIN      
        1 tttcgagacg ttttttcatt aaatccacac tgacgagggt aaaatccttt ccgctctcac
       61 gcagggtgat atgggaagcg agagagcagg cacccggttt gtagaacaat ttcatcggta
      121 actccttttg tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaagccgcta
      181 acgaaaagtt agcggcttgt aaaagagttt ccctgaattt tacgctacag cagctttcgc
      241 cgctttgtct gcagcgtcat cctgcgtcat acggtgcagt ttaggcgcgg tcagcagcat
      301 cagtactgca ataatggcag tagcgacacc aatctgcaag aatacgcgac cataaacttc
      361 cagtgacatc agcgggtcgg taacgttatc cggcacagcc atcatacccg caacataacc
      421 accaatcagg tttgcaccgg cagtggtcag gaaccagcta cccataatga agcccatcag
      481 acgctgcgga acgagttgtg caaccattgc cagacccaga ccagagatca tcagttcccc
      541 gatgctctgc aggccatagc ttgcgaccag ccagcttaca gacacgatac cagcgtcaga
      601 cgcgaatttc gcacccagcg gcagaatcag gaacgcacca gaacacatca ccatgccgat
      661 tgcaaacttg gttggcatcg gcagggtatc gcccatcttg ttatagatag cggccagaat
      721 cggactaccg atgatgatcc agaacgggtt cagtgcctga tactgctcag gttctacggc
      781 cagacccaga atggagtgct caacgttacg aatcgcaaag aagttcagtg acgttggcat
      841 ctggctgtac agcacgaaga agataatggc ttcgagcatc aggatgaagg caacgatcat
      901 tttacgacgc gcagcacctt tcatcgcgaa ggcttcttta ccgaagataa ccacgatacc
      961 gaaggcaaca acgcccagcg ccatacgcgc aacttcctga ttgtgcagca gccaggtggc
     1021 gatagcgatc agtgccacaa caccaataat ggtcagcagc aggttacggt agttgatagg
     1081 ctcgaagtct ggttttgaac cgtactgttt aacccagcgt tggcagaagg cgaagttaac
     1141 gatagtgatc agcaggccta caacgctcaa cgcaaacgca acactccagc cgtatttcgc
     1201 ggccagccac ggcgtagcaa tcatagagaa gaaagagccg atgttgacgg acatgtagta
     1261 catggtgaat gcaccgtcca gacgcgggtc gtttttctca tagcatgtag aaagcagaga
     1321 agatgggtta gctttaaaca ggccgttacc gaccgcaata gccgccatac ccatataaac
     1381 gataccggcg tcgtgaccag accaggcaac cagagcataa ccaatcgcca gcacaatagc
     1441 accgagcata attacgcgtt tagtacccag taccttgtca cctaaccagc cgccgatagc
     1501 gaccagacca taaaccaggg cactaaagga agagaaaagg gtgattgaat ccgcttcaga
     1561 catacccagt tgtttaacca ggtaaacagc cataattcct tgtaggccgt aataaccaaa
     1621 acgttcccat aactcaatcg agaagatgag atagaacgcc ttcggttgtt tgaaagcgtt
     1681 caaactgacg ctttcagttg gtttttggtt tgcagtggac acttttacct ctttttttac
     1741 atcccatatt aacgggggtg ctttctcgcg ttatgtctaa aaaacatacg cgcaattgtt
     1801 atagtgggag gggaaacggc aggtaatgtt cactatcttg cactttcaga caagtccttt
     1861 gtaataatct gttacatata actgaggcgg ggaattcaac cattccggga aatgttatcc
     1921 agcgttaaat ttttcaaaaa acgtaatggc agattttttc actgcactga gaggggggtg
     1981 atggcttaac ggcgatatgt tctgctattt agcgttttta ctagtgagat agtccagttt
     2041 ctgaaaaata gccagtgtaa tgttttgtag gtcaatattt gttgcgtgtg taaatttaaa
     2101 ttattttaac ctgattcttg caatgttttt acattgcgac agcatcgtga tcttgatcac
     2161 gaatgaatag aaaatttcgc tattaaaaaa acaattaacc tgcatttttg gttatccgca
     2221 aacggattac ctgatgataa cgctaagcaa tggcatcatt cacgttaaaa agatgtttta
     2281 aaagaggata aagaaaggtt atcaatgggg taatcggtgt tacccctttt cttcagatgt
     2341 caactttctc tttgtattca caaagatctt caataataca agagccacag cggggcttgc
     2401 gggcaatgca ggtataacgc ccgtgcagga tcaaccaatg gtggcagtcg actttaaact
     2461 ctgccggaac cactttcagt agcttttctt ctacctgttc gacgtttttc cccggcgcaa
     2521 attgagtacg attacaaacg cggaaaatgt gcgtgtcgac agcaatagtc ggccagccga
     2581 atgcagtgtt taatacgacg ttggctgttt tacgacctac gccgggcagg gcttcaagcg
     2641 cagcacgatc ttccggaacc tcgccattat gctgctccag taagatacgg caggttttga
     2701 tgatattttc tgctttgctg ttataaagcc caatcgtttt gatataggtt ttaacccctt
     2761 caacgcccag ttcaagcatc gctgcaggcg tattcgccac cgggtaaagt ttcgccgtcg
     2821 ccttattaac actgacatcg gtcgcctgag cggaaagcag tacggcaatc agcaattcaa
     2881 aaggcgaact gaaattaagc tcggtggtgg gatgaggatt gttctcacgc aggcgagtga
     2941 ggatctccag gcgttttgct ttgttcatca gacattccct gtttcaccgt ttggcaatgc
     3001 acgttcggca gctgcttcag tacggcgctt tttcattctt tcatcaatca ggtattttcc
     3061 tgccagcatc agtcccaggc caataaatgc acctggtggc agcatcgcca gcaggaaagg
     3121 ggagtcggtg tggaaaatct ccacgcgtaa tacttttgcc cagctaccta acagcgcatc
     3181 tgcaccgtca aacaatgtgc cattgccgat aatttcgcgt agtgaaccca gcacgaacat
     3241 ggcgcaggtt gcgcccatac caattgaaaa gccgtccagt gccgaaagcg ccggaccttt
     3301 tttggcggcg aaggcttcag cgcggcccac aacgatacag ttagtgacaa tcagcggaat
     3361 aaaaatccct aatgattgat acaggccaaa ggcgtaggcg ttgatcagca tctgtacagc
     3421 gctgaccacc gaggcgatga tcatcacgta aatgggaatg cggatctcgg ctggcgtcca
     3481 gtgacgcagc gtcgaaatgg tcaggttggt cagcgtcagt accagcgtag tcgcaagtcc
     3541 taaacccaga gcgttagtgg cagtggacgt gaccgccaac agaggacaaa ggccgagcaa
     3601 ctggaccagc gcagagttgt ttttccacaa cccctgaaca ataacgtctt taatttcgct
     3661 cacgctttat tctccacagg caggaagttg agaaagttgt gccggtaacg tctgagcgta
     3721 caatccggcg cgttttaccg cattaaccac cgcgcgggga gtaatcgtcg cgccggtgaa
     3781 ctggtcgaaa tcaccaccat ctttcttcac cgcccagtgc gcatcatccg caccactgat
     3841 ttttttaccc gcaaaatggg tgatccagtc agaaaggcgc agttcgattt tatcgccaag
     3901 ccctggcgtt tcgtggtgct ctgtcacgcg cgtgccaagt accgtgccgt taaaatcggc
     3961 tcccaccagc agctgaatcg caccggaata gccatctggc gcggttgctt ccagaacggc
     4021 ggctaccggt ttgtcatcct gtttggcgat gtaaacccga tgctcacctt tacctaactc
     4081 tggcgcagtt accagatagc aactctgtgc cagcgtattg ttatagcgtt cggctggcag
     4141 cacctgatca aataatgcct tttgttgcag actggcctgt tcagcaatcg tcgttttggt
     4201 catctggttg atggccgcag ttaaccctgt tgaacccgct gcaaatagcg ctaacgtaat
     4261 gccgtgtttt cggatagttt tcagcatggt ttaccctttg cgatggccgt agacgcgcgg
     4321 acgcgtgtag taatcgatca gaggaaccgt gatgttcgcc agcaggacgg caaaagccac
     4381 gccgtcagga tagccgccga aactgcggat caaccagact aataagcccg caagcgcgcc
     4441 gaaaataaga cgaccacgat tggtcgtaga agcggtaacc gggtcagtca aaataaagaa
     4501 tgcgccgagc atggtcgcac cagacagcag atgaatttgc ggtgctgcca gtgtttctgg
     4561 tgagaacaac cagcccaacg ttgcgcataa cgccagcgtt actaagaagc tgaggggaat
     4621 atgccagcga atcgctttct gccatagcaa ccacaggccg ccagccagcc aggcgagatt
     4681 tacccattgc caaccagcgc ccgccagaat accgctgtag atcggatatt gcataatctg
     4741 ttcaaccgaa tgaccggcac ggacagaggt tttaaatgta tccagcggtg tcgcctgact
     4801 aatgccatca atacctaagc gtagtgtgtt catatcacca ccactggcgg tatgcccgct
     4861 gaaaataacc tgtatggcgt cgataaaacc agggatgttg accgcaattt catgtggcgg
     4921 taaccagctg gtcatctgca ccgggaagga gatcagtaag accacataac caatcattgc
     4981 cggattaaac gggttttgcc ccagaccgcc atacaactgt ttagcgatga tcaccgcaaa
     5041 caccgtaccc agcacgacca tccaccatgg cgcgaggggg ggaatactta ccgccagcaa
     5101 taagcctgtc agcaatgctg agttatcttt caacgttgcg gctaccgact gcttgcgtag
     5161 tttgagtacg agagcttcgg ctaacagagt actaaccgat gccaacagga tctgaacgag
     5221 agtaccccaa ccaaaaaacc acagttgcgc tgcgattcct ggcacggctg cgagcaacac
     5281 caacagcata atgcgcgatg tctggcgctg gttatgggta taaggggagc tagctattct
     5341 gaataccatt tagtcctcgt ttacaacctt ctgctgggcg gcttttttgg cctgagcgcg
     5401 ggcaatagcc gcggcaactg c
//
LOCUS       DAJVQG010000202         5218 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJVQG010000202 DAJVQG010000000
VERSION     DAJVQG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.203"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(1..1123)
                     /locus_tag="OQM83_003715"
                     /pseudo
     CDS             complement(1..1123)
                     /locus_tag="OQM83_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1123..1305)
                     /locus_tag="OQM83_003716"
     CDS             complement(1123..1305)
                     /locus_tag="OQM83_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521863.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1317..3050)
                     /locus_tag="OQM83_003717"
     CDS             complement(1317..3050)
                     /locus_tag="OQM83_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCS1521864.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3047..3541)
                     /locus_tag="OQM83_003718"
     CDS             complement(3047..3541)
                     /locus_tag="OQM83_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCS1521865.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3663..4013)
                     /locus_tag="OQM83_003719"
     CDS             complement(3663..4013)
                     /locus_tag="OQM83_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1521866.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4056..4520)
                     /locus_tag="OQM83_003720"
     CDS             complement(4056..4520)
                     /locus_tag="OQM83_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCS1521867.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(4517..5014)
                     /gene="rrrD"
                     /locus_tag="OQM83_003721"
     CDS             complement(4517..5014)
                     /gene="rrrD"
                     /locus_tag="OQM83_003721"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HCS1521868.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(5014..>5218)
                     /locus_tag="OQM83_003722"
     CDS             complement(5014..>5218)
                     /locus_tag="OQM83_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCS1521869.1"
                     /translation="EKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGSLLLG
                     LLTYLTNLYFKIREDKRKAARGE"
BASE COUNT         1141 a         1527 c         1292 g         1258 t
ORIGIN      
        1 ttcatatctt ccaggtcgcg gcagtcattg gaagagtaaa ttccccagtt gatcccggtg
       61 gcgtaggctt caaaacggga cttcatatcc ccgcgcagta acgccccggc gttaaatttg
      121 gcgtaataaa cgccctgctt actttttcgt accagtccgg tgttgatccg ctgttcgatg
      181 cgggtcagat acggcaccag tgaatagttg ataaatccca gccccagctc ttcgatattg
      241 ttgaaggtgg cgcgatcggt gttctgcacc atgtgcaacg gcacccggaa cagacgacag
      301 atttcttcca gctgaaactt gcgggtttcc aggaactgac tgtcctcggc gttcagcgcc
      361 atcgacttcc agtccagccc catttccaga atcatcgggc ggtgagcatt gccaagcccc
      421 gtgtgacact cctcaaaatc tttcttcaga cgctcgtaag catccggcgt gagcttttgt
      481 tccgtacgca acacaccgga tgtcaccgca ccattactaa acaacctggc accgtgctcc
      541 tcggttgccg ctgccagtga aatggcctca cgcgcatacg caatgggatt cagcccgaca
      601 agtccatcca gcgtcagggt gcgcacatgc cagatttcat tctgggtcaa cacatccacg
      661 gaaccatccg gaaacgtcac ctgataaacc ggctgccact ggctgttcag cttcggttcc
      721 acacagcccg gatctatcgg aagaagctcc accacttctc ccagtgcctt taccttgtag
      781 gcgtaaaaat tcccccgtag acacaggcag acaataacca gctcccagaa ttcctgcggt
      841 gtcatgtagc cattaggttt tgccgaaacc aacttatgca gtcgttcatc caccgcccgt
      901 gttttcaggg taccgctgat tttgtagaga ctgcagggca gcataccaac agactctgcc
      961 agcaccctga cgcaagaata gaccgccgtc aggcgcatgg cccgctggct actgatccgc
     1021 tttccggtat aggtgtcgta tgacagcccg atagcatcag cagttctgct ggcgtggtca
     1081 ccggcacatc gctttttcgt tgaaataatc ccgaaaagaa cactatttac ctccgccgac
     1141 agactgccgt gtacggtcga gatatcgcgc caccagccac gaccagaaca ggcacagcgc
     1201 cccggcaaca acaaaacccg ccgggggata aatcagccag gcaccatacg ccagcaaaag
     1261 cgcacccagc acgcccacca gaggcgcgag aatcagcatg atcataatta cctcagttaa
     1321 agcgagcgga tcccgtagga ctcaatgtgg tcagacagcg tgtcttcttt ctcgtacagc
     1381 atggctctgc caaccgccat aatcagcgca actgcaccat cgattttgtt ttccgcctgc
     1441 tctttgacgg gtttcaccac atcatcgtta cccggcatgt ttttgccgac cacgttgccg
     1501 atacaccagg tcatgatggg attgccgtca tgatgaaaac gtcccgattc aatcgctgct
     1561 tccagctctt tcatcggatc ggacatattg gtgaagttct ggacgatagt gacgggattc
     1621 aggtcttcat cagcaaggtc atgtgacaac ccggtcgccc cgaaggggtc gatgggtgac
     1681 tcactgaccg ggctgatttt gttcgccgct ttggcctcct cgaggatgta gcgataatcc
     1741 acctccgcac catcggtaac ggtcagaacg cccatttcca cccatttctg aaagcgttcg
     1801 gctgtccgtc gatcttcatt tttctcgacg ctgtacaccg tgtcatacgg tacccagaaa
     1861 cgcggggcca cactgtagta atgcgtttta ccgtcaatct cgcgggtata aagtcgcgcc
     1921 atgctgttca tatccagctt acgcgccagg tcaaaggcca gaatgcacgg ctgcccctcg
     1981 aactgctcaa gggtcagtga tttatcctcg cagctctgcc agctcaccaa gttgaaatac
     2041 gccgaacgcg ccgacaccca gatattgagg tgttttgttt taaagacgtt tgccagacgg
     2101 gcgttatttt tcgcacgctg ctgctgactt aacaaaaatt cgcgataaac cgacacgcca
     2161 atatttggat tggctttttc cagcacctgc gggccggtcc agtcgtcacc ttcatcaacg
     2221 gtatagatga tcccgaacag ttcatcgtta ggcaccgagc cgttgagcat ctcgatgact
     2281 tcccgccgct tgtcgtagca cggcccctca atgttgtacc cggcggtggt gatggcccac
     2341 atcagtggct gacgtcgcgc ccccatcccg gtaagcattg tggtataaag cgcatcggtg
     2401 gcatgctcgt gatattcatc aaccacggca cagtggggtg atgaaccatc acctgggttg
     2461 ccgatcagcg gttcaaaccg cgcgccatcc tccggacggt tcatgtttga ggcgttaacc
     2521 tcaatcccga acgcttccgt cagcatgggt gtgcgtttac acatcagtcg cgccgggcga
     2581 aagacttccc acgcctgttt ctctgtcgtg gcaccggaat acacttccgc gccaaactcg
     2641 ttatcacagg caaaacaata cagggcaaca ccggcagaga ttgctgattt gccgttctta
     2701 cgggggattt cggtgtacac ctcccggaag cggcgcaacc gggtgccttt attgacccag
     2761 ccaaacgcac agcagatcac aaatagctgc cacggctcca gcgtgatggg catccgtttg
     2821 aatgcccact cccccttggt gtgcggcaac agctgaataa atttcgcggc ccgttcagcc
     2881 aggtccttgt cgaagcggta acgaaacgac ttactttttt ccgccatcag gtcatcaaga
     2941 tggcgctggc aggcctgaat cacaaactgg caggcaacaa tctttccgcg cacgacatcc
     3001 cgggcatact gatttgcagc atttacgttg gggtaagatt tccggctcat gattcgataa
     3061 ttttcagaaa cgggttagtg gctttcttct gccccgccag gccaatcaga cgctggcggc
     3121 tgctggggtc gagtccgagc attgcccccg tgctgctcat ctcggactcc tgttcttttt
     3181 tggcggtcag ctccggattt ttgaccctgc cgcccattgc accggtgatg gtgttgccct
     3241 gtatggcaat atttttcacg gcacgtcgcc agaactcata ggccacgcac caccgctcaa
     3301 gtaccgccag gtcagtcacg cacagcaggc cctgaccgca gagttctttg gttgtcagtt
     3361 gccacatgat cgtggcgaga gggagatctt cttcagcgaa ccactctggt ggctcaacac
     3421 ctttgatggg cgtaaaaaca ggttcatctt tgttcagggc tcgcttgccg gggtttccgg
     3481 ccagcgcctt gcgcgccgtt ggcttggggc gacgcccgga acgccccgcc gttccagcca
     3541 tatgcggcac tcctggttaa atttcatttt tcgcgggtat aaaaaaacga tggggcgggc
     3601 agtccggaag acgtcaggtc acagggattt gccccgcccc tccctacaag tgagaataat
     3661 tatcaccgga ttcgttcgcg cgctgttttc gctttatggc agggccagca cagactctgc
     3721 aggttgctgt ctgcgtctgt tccgccatgc gctttcggga tgatgtggtc gacggttttc
     3781 gcctcgctca ccacaccgac acgcagacac aactgacaca gacctttatc gcgcttcaga
     3841 atacgggcac gaatcaccgt ccattttgag ccatagccac gctggtggcg gctcagtccg
     3901 cgctggtgct gcacccagcc ttcaccgcga tgtttatcgc agtaacccga actgtctgtg
     3961 gttgtacctg cacatccacg cttacggcag gcacgtggga ttagtgatgg cataaatacc
     4021 tcataccctg cgaaatgttt accacgataa aaaggctact taatgcactg agtgcggata
     4081 tactcctgtg ccccttccag ttgcatctgc atcgtcatca gccgctctct gagggtgaaa
     4141 taatcccgtt cagcggtgtc tgccagtcgg gggctggttg cattatccac gccggaggcg
     4201 gtggtggctt cacgcactga ctgacagact gctttgatgt gcaaccgacg acgaccagcg
     4261 gcaacatcag cgcgcagagt ttcattttca gctttcgcat cagctaactc cttcgtgtat
     4321 ttagcatcga gtgcattaac atcacgctgt ctctgctgca tgtcagtaat ggttgcgttc
     4381 gccagcttca gttctcttgc gtttttgtcg cgctgctctt tgtaggtaat ggcgttatca
     4441 cggtaatgat taacagccca tgacaggcag acgatgatgc agataaccag agcggagata
     4501 atcgcggtga ctctgctcat tgctgccccc acaaacagac ttcacgctca atctcacgac
     4561 gggtcatcag ccctttccat tgcttaccgc cagcatatgt ccagcgacgt agctggtcac
     4621 atgcgccttt gatatcgccc tggtttattt tgcgaagaag cgtcgatgtt ctgaaattgc
     4681 cagcgcccac gttgtaaacg aacgagtaaa gagcgccgcg cgttgtttcc ggtatatcga
     4741 ctttgatgta cgggttaatt tgtctggcga cagtggcaag gtctttattc aggagggctt
     4801 tgcattctgc ttcggtatac gttttaccga gcatgatgtc ttttccggtg tgtccgtgac
     4861 atacagtcca tacaccaatg atatctttgt atggtatgta gctgacacct tccagaccat
     4921 cgttaccacc tgggccagtg attaacacag atgctatagc aacagcccca ccaccaatag
     4981 cagctgcaac agccttgcgt aatgatggcg acattattca cctctcgcag ccttacgctt
     5041 gtcttctctt attttgaaat acagatttgt cagataagtc agaagcccca gaagcagact
     5101 tcccagcaca ccaatcgcag cccactgtga tggactgacc tgatccaacc actgcaaaaa
     5161 ccagtagccg gcactgccgg cggaggtgcc gtaggcaatg cctgttgata ttttttcc
//
LOCUS       DAJVQG010000203         5206 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJVQG010000203 DAJVQG010000000
VERSION     DAJVQG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.204"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..619
                     /locus_tag="OQM83_003723"
     CDS             <1..619
                     /locus_tag="OQM83_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCS1521870.1"
                     /translation="QKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIETVGVNQ
                     IETVGSNQIIKVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSL
                     RVGLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLN
                     GTKIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
     gene            687..4229
                     /locus_tag="OQM83_003724"
                     /pseudo
     CDS             687..4229
                     /locus_tag="OQM83_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT         1268 a         1285 c         1657 g          996 t
ORIGIN      
        1 gcagaagaac atgaacaccg aggtgctgaa taaccgcacc acggatgtga taaacaacca
       61 tgctgaaacc attggcaaca atcagatgat tgcggttacc aacaatcaga tagagacggt
      121 gggcgttaac cagatagaga cggtgggcag taaccagata attaaagtgg gttctgttca
      181 ggtggaaacg attggacttg ttcgtgcgct gacggtgggc gtggcgtatc agacgacggt
      241 aggtggcatt atgaacacct cggtggcact gatgcagtcc tcgcagatcg gtttgcataa
      301 atcgttgagg gtcgggctgg gttatgacgt caaagtcgga aataacgtta ccttcaccgt
      361 tggtaaaacg aaaaaggatg ataccgggca gaccgcgatt tactccgccg gtgagcatct
      421 ggagctctgc tgtggtaagg caaggctggt gctgacgaag gacggacaaa tttttctcaa
      481 cggcacaaaa attcatttgc agggtaagga gcaggttaat ggtgactcac tgttgattaa
      541 ctggaactgt gcagcctcga aatccccacc gaagacccct gatgaaaagc aggatacgcc
      601 ggatatgaga gagtactgat cccgtacctg ttcggtattc agtaacaaac cagcattaaa
      661 aataaacaga agaaggaatt tgaatcatga gcggaaaacc agcggcgcgt cagggagata
      721 tgactcagta tggcggtccc attgtccagg gttcggcagg tgtaagaatt ggcgcgccca
      781 ccggcgtggc gtgctcggtg tgtccgggcg ggatgacttc gggcaacccg gtaaatccgc
      841 tgctgggggc gaaggtgctg cccggcgaga cggaccttgc gctgcccggc ccgctgccgt
      901 tcattctctc ccgcacctac agcagctacc ggacgaagac gcctgcaccg gtgggcgttt
      961 tcggccccgg ctggaaagcg ccttctgata tccgcttaca gctacgtgat gacggactga
     1021 tactcaacga caacggcggg cggagcattc actttgagcc gctgctgccg ggggaggcgg
     1081 tgtacagccg cagtgagtca atgtggctgg tgcgcggtgg taaggcagca cagccggacg
     1141 gccatacgct ggcgcggctg tggggggcgc tgcgccggat atccggttaa gcccgcatct
     1201 ttacctggcg accaacagcg cacaggggcc gtggtggata ctgggggggg ctgagcgggt
     1261 gccgggtgct gaggacgtac tgccagcgcc gctgccgccg taccgggtgc ttaccgggat
     1321 ggcggacgct gacgtaccgg cgtgaggccg ccggtgacct ggccggggaa atcaccgacg
     1381 tgacggacgg tgccgggcgg gagttccgtc tggtgctgac cacgcaggcg cagcgtgcgg
     1441 aagaggcccg cacctcttcg ctatcttctt ctgacagttc ccgccctctc tcagcctcag
     1501 cgttccccga cacactgccc ggtaccgaat acggccccga caggggtatc cgcctttcgg
     1561 cggtgtggtt gatgcacgac ccggcatacc cggagagcct gcccgctgcg ccactggtgc
     1621 ggtacacgta tacggaagcc ggtgaactgc tggcggtata tgaccgcagc aatacgcagg
     1681 tgcgcgcttt cacgtatgac gcgcagcgcc cgggccggat ggtggcgcac cgttacgcgg
     1741 gaaggccgga gatgcgctac cgctacgacg atacggggcg ggtggtggag caactgaacc
     1801 cggcagggtt aagctaccgc tatctttatg agcaggaccg catcaccgtc accgacagcc
     1861 tgaaccggcg tgaggtgctg catacagaag gcggggccgg gctgaaacgg gtggtgaaaa
     1921 aagaactggc ggacggcagc gtcacgcgca gcgggtatga cgcggcagga aggctcacgg
     1981 cgcagacgga cgcggcggga cggcggacag agtacggtct gaatgtggtg tccggcgata
     2041 tcacggacat caccacaccg gacgggcggg agacgaaatt ttactataac gacgggaacc
     2101 agctgacggc ggtggtgtac ccggacgggc tggagagccg ccgggaatat gatgaaccgg
     2161 gcaggctggt atcggagaca tcgcgcagcg gggagacagt acgctaccgc tacgatgacg
     2221 cgcacagtga gttaccggcg acgacaacgg atgcgacggg cagcacccgg cagatgacgt
     2281 ggagccgcta cgggcagttg ctggcgttca ctgactgctc gggctaccag acccgctatg
     2341 aatacgaccg cttcggccag atgacggcgg ttcaccgcga ggaaggtatc agcctttacc
     2401 gccactatga caaccgtggc cggttaacct cggtgaaaga cgcacagggc cgtgaaacgc
     2461 agtatgagta caacgccgca ggcgacctga ctgccgttat caccccggac ggcaaccgga
     2521 gcgagacaca gtacgatgcg tggggaaagg cggtcagcac cacgcagggc gggctgacgc
     2581 gcagtatgga gtacgatgct gccggacgtg tcatcagtct gactaacgag aacggcagtc
     2641 acagcgactt cagctacgat gcgctggacc ggctggtaca gcagggcggc tttgacgggc
     2701 ggacgcaacg ttatcattat gacctgaccg gaaaactcac gcagagtgag gatgaggggc
     2761 ttgtcaccct ctggcactac gatgcgtcgg accgcatcac gcaccggacg gtgaacggcg
     2821 acccggcaga gcagtggcag tatgacggcc acggctggct gagagaaatc agccacctga
     2881 gcgaaggcca ccgtgtcgct gtccactacg gctacgacga taagggccgc ctgacgggcg
     2941 aacggcagac ggtggagaac ccggagacgg gggaactgct gtggcagcat gagacgaaac
     3001 acgcctacaa cgaacagggg ctggcaaacc gcgtcacgcc ggacagcctg ccgccggtgg
     3061 agtggctgac ctacggcagc ggttacctgg cgggcatgaa gctgggcggg acgccgctgg
     3121 tcgagtatac gcgggacagg ctgcaccgtg agacggtgcg caccctcgca ccggacctgg
     3181 acatccgcat cccgtatgcc accgacccgg cgggaaaccg cctgccggac ccggagctac
     3241 acccggacag cacgctcacg gcgtggccgg ataaccgcat cgcagaggat gcgcactatg
     3301 tttaccacta tgatgaatac ggcagactga cggagaagac ggaccgcatc ccgacgggtg
     3361 tgatacggac ggacgacgag cggacccacc actacgacag ccagcaccgc ctggtgttcc
     3421 acacgcggat acagcatggc gagccactgg tcgagagccg ctacctctac gacccgctgg
     3481 gacggcgaat ggcgaaacgg gtatggcggc gggagcgtga cctgacgggg tggatgtcgc
     3541 tgtcgcgtaa accggaggag acatggtacg gctgggacgg cgacaggctg acgacggtac
     3601 agaccgacac cacacgtatc cagacggtat acgagccggg aagcttcacg ccgctcatcc
     3661 gggtcgagac agagaacggc gagcgggaga aagcgcagcg ccgcagcctg gcggagaagc
     3721 tgcagcagga agggagcgag gacgggcacg gcgtggtatt tccggctgaa ctggtgcggc
     3781 tgctggacag actggaggaa gaaatccggg cagaccgcgt gagcagtgaa agccgggtgt
     3841 ggcttgcgca gtgcgggctg acggtggagc aactggccag acaggtggag ccggaataca
     3901 caccagtgcg aaacgttcat ctttaccact gcgaccatcg gggccagccg ctggcgctca
     3961 tcagcgaaga cggcgatacg gtgtggagcg gggcgtatga tgagtggggc aaccagctaa
     4021 atgaggagaa cccgcatcat gtgtatcagc cataccgtct gccggggcag cagtatgatg
     4081 aggagtcggg gctgtactac aatcgtcacc ggtactatga tccgttgcag agacggtaca
     4141 tcacccagga ccccattggg ctgaaaggtg ggtggaatct gtatcagtat ccgctgaatc
     4201 cggtgagcag gatagatccg ttggggttat tctcatgtga gagttgtagt cattttatag
     4261 atgatataaa taataaactg gataaccttc cgtttaaccc gacaaaaatg tttacagcta
     4321 gtttaaatac tgctaattca gcaaggctgt atgcaagtgg catgacaaaa ttcggtataa
     4381 gtgttgctgc cgatggaacg atcattgctg ccccagtcgg tgcagcttct atggctttag
     4441 gggcgtggaa tataaaatct gcgacagctg catataacag agcaaattta caattatcag
     4501 aatcacttca ggaaaatggt agagataggt gttggagaaa tttattgggt attttacctt
     4561 atggtgagca gtttgatgac cctggtgaac catcaatatt agacgtttat ggtgaaaaga
     4621 ttgagaatat caaagattct ccaatgaagt tttttgaaga gcttgggacg ctggggttgt
     4681 aatgtgtatt tttaatgata tttttatgtt tgtgtttatt gttagtagta tacttgtaac
     4741 cgtgcatttt gtgtattctt tattttttaa aagtggaaag gtaaaagata aggtaaaaaa
     4801 atggattaaa gtaatgtgtg atttattttg ggggatagga tagtggtgtg atatggataa
     4861 atataaatta cagtggctgg catatttcga cctgacgccg gagtagataa aaaaacagac
     4921 aggattggag tatagcaagc gtctgacaat ctctcgggac agtagtatga tgaagaggcg
     4981 gggttgtact ataaccgtaa ccggtactac gatccgctaa agggcaagta tatgactcag
     5041 ggaaagatca tcagaagttc actttttgta ctaaataatt cgcattttat gtttaaaaat
     5101 tgagatattc ctcattacct gaagctgttt ttttattgct tatacataat caaatactcc
     5161 ttacataatt aaggagaaca aaatggaaat taaaaaattg atggaa
//
LOCUS       DAJVQG010000204         5201 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJVQG010000204 DAJVQG010000000
VERSION     DAJVQG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.205"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(84..755)
                     /locus_tag="OQM83_003725"
     CDS             complement(84..755)
                     /locus_tag="OQM83_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HCS1521871.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(862..1095)
                     /gene="yedF"
                     /locus_tag="OQM83_003726"
     CDS             complement(862..1095)
                     /gene="yedF"
                     /locus_tag="OQM83_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCS1521872.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1092..2297)
                     /gene="yedE"
                     /locus_tag="OQM83_003727"
     CDS             complement(1092..2297)
                     /gene="yedE"
                     /locus_tag="OQM83_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCS1521873.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2484..2897
                     /locus_tag="OQM83_003728"
     CDS             2484..2897
                     /locus_tag="OQM83_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1521874.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEEIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(2931..4418)
                     /gene="amyA"
                     /locus_tag="OQM83_003729"
     CDS             complement(2931..4418)
                     /gene="amyA"
                     /locus_tag="OQM83_003729"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCS1521875.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4496..4861)
                     /gene="fliT"
                     /locus_tag="OQM83_003730"
     CDS             complement(4496..4861)
                     /gene="fliT"
                     /locus_tag="OQM83_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCS1521876.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(4861..>5201)
                     /gene="fliS"
                     /locus_tag="OQM83_003731"
     CDS             complement(4861..>5201)
                     /gene="fliS"
                     /locus_tag="OQM83_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCS1521877.1"
                     /translation="QQLVTMLFDGVLSALVRARLFMQDNNQQGKGVSLSKAINIIENG
                     LRVSLDEESKDELTQNLIALYSYMVRRLLQANLRNDVSAVEEVEALMRNIADAWKESL
                     LSPSLIQDPV"
BASE COUNT         1304 a         1392 c         1290 g         1215 t
ORIGIN      
        1 cgttgaagca ggaacccacc gaaatgattc aggcgacagc ctgaacgtag cagggatcct
       61 cgtccttcag ggcgtggagg atgtcaacaa acaggttgaa ttaactccgc gccctggttt
      121 ttgacattac ctaccgcgcg cgataccggg tgccaggtga actggtttgc tggcacacag
      181 ccgttggtag caatttctga tgcttcttta ccgccaatct cttgccgcat ccattcctgt
      241 gcagcttctg gcgacagaac cagcgggcgg cggtcatgaa tgtctaccag accctggtct
      301 gctgcagcag tgacaattaa aaatccttcg gcttcgtcac cacgctcaaa tggtgtgcta
      361 cctatcgcgg ccataaaaat aggttgtcca tcagcacgat agataaaaaa aggctgtttt
      421 ttgtcgcctt cttttttcca ctcaaaccag ccatcggcaa aacagattgc ccgaccatgt
      481 tgccagagcg gtttaaacat gcgactggtt gcggcagttt ctacgcgggc gttaatcagc
      541 ggcggtttat cccaccatcc gggagcatat ccccagaaaa ccggatccag atgaaggtgt
      601 tcatcacgtt cactgagcag aagaactttg gttcccggcg cgacgttgta tctgccaatg
      661 ggttcgggat cgtagggaat atcgcgttca atatcttccg cgagaagcgc aaggtaatct
      721 tcacgcgttt gggattgggc aaagcgtcca cacatagaaa cctcctgaga tcatactgaa
      781 aagaatagga gagggggcgg gtaaaatcta tgtgtcagag gcaacaatgt tcccgctgtg
      841 ggccacagcg ggaatgaggg attacttctg aattaaataa cgaatggtcg gcccgtcttg
      901 ctgaatatcc agcaccgtat agccgtgatt acgcgcatcc agtggaatat tattgatcga
      961 ctgtggacag tcgctcacca cttccaggat ttcccctttt tttaactgcg gcatcgcctc
     1021 aagggttgcg actgccggat aagggcaggg ttcacccacc atatcaaggc ggtaatcagg
     1081 aacgatattt ttcatgcgat ctccttagca gtctgcggtg ccgcacggcg gaagaagcgt
     1141 ttttcccagc cgataatcaa catcagcgca gtaaacaaca gcaaatatgt caccagcagg
     1201 ccacccatcg ggccaaaggt tttcagcagg ttgattttgt cccagtcggt ggccagcgcc
     1261 ggagcgaaat catcccagta atacgccaga atcgtcgagc cgatcacatt gcccagaccg
     1321 acccaccagt agtgcacctg gccttctacc gcgcggtaca tccagccggt ttcgcagccg
     1381 cctgccagca cgatgccaaa accaaacagt aaaccaccaa ttaccgcgtt tggtcccgcc
     1441 cacatgattt tgggttcaac gcctaactgt acgtaactga agatcccgat ggcactcacc
     1501 gccataccga taatgattgc tttcgccata tgggtacgtc cggtgatcca catatcgcgg
     1561 aacgccgaag taaagcagat ttgcgcacgt tcaatcagta aaccaaagcc gacgccaaac
     1621 agcattgcca gccccagttt gggttggttc atcgccgtga gcagcgccca gcccagcatg
     1681 ccgaaaaaga ccagcatccc gagacgaaaa cgacgccgcg cctgatccgg tttttgcgtc
     1741 agcggtgagg cggcagaaac tttctgcatt ttcacgggaa tacggaagat gggcagcagg
     1801 gtaaagcgcg cgccaaacca tgaaccaatg gcagtggcga tggcaaagaa ccaggcatgc
     1861 agcgagaact gaggaatgcc ggtaaagaac gccgccaggt tacagcccat tgccagacgc
     1921 gcgccaaaac cggcgataat gccgccaata atggcctgca taatgcggat acggctgcgc
     1981 ggcatgcgca gtttgacatt gttggcccac agcgctgcgg caaagcagcc gccaaacatg
     2041 ccgaggatca tcatcccgtc gatgcgggtt aatggcgatc cttccagatg gataatttta
     2101 aaataacccc actcttcagc atggacgcca aacagctgca ggagctgacc gccccaacgg
     2161 gtaaattcac ccgtgacagc ccaaaaggtg ccagtaatgc caaaatagta agtggagaga
     2221 atacccgccg cgatgaccgc agggatgggt gcccagaatt taatcaacca ggcgtgtttg
     2281 aattgctgcc atgacatgaa tgtagcctct gaactcagaa ggtttgaaac aagaatcgcg
     2341 aatcatacat catgcaaatt tattttcggg gtaataattc acaaaaataa tagttagatc
     2401 aaatttctct ctgaacctgg aaaggctggc gacatcaggg ctttaatgcc acaatcctct
     2461 tttcttctca tgcaggacga atgatgaaaa aactcgcaat tgcaggtgcg ctcatgttgc
     2521 tggcgggctg tgccgaagtc gaaaactata acaatgtcgt gaaaaccccc gcgccggact
     2581 ggctggcggg ctactggcag accaaagggc cgcaacgtgc gctggtcagt ccggaagaga
     2641 taggcagcct gattgtcact aaagagggcg ataccctgga ttgccgtcag tggcagcggg
     2701 tgattgccgt gccgggcaag ttgacattga tgtctgatga tctcactaac gttaccgtaa
     2761 aacgtgagct gtacgaggtt gagcgtgacg gtaacaccat tgagtacgat ggtatgacga
     2821 tggagcgtgt ggatcgcccg acggcagagt gtgccgccgc gctggataaa gcgccgttac
     2881 cgacgccact gccgtaatga aatctccccg gcttgccgcc ggggatgaat ttaaatcacc
     2941 tcttcgataa cccacacgct gacgctgccg ccgttgcaaa agaaggtcgc ttcgccgttt
     3001 tcgtcggtca ctacgctctc ttgccgattc cccagaaaat cacgccaggt tttattgccg
     3061 taattctctc ccagatgaat ggttttttcg ccatcatccc cgttcgacat gaccaccacg
     3121 cagccgggat attcgtcagt gccactgcgg ctaaaggcaa tgcagttcgg atggtcgaaa
     3181 aataacgtct gtacaccgtg ggcgaaacgc tgacgggcga gaattaactc atcaagctgt
     3241 tcgattattg gcatatctat cggataggtt tgcccgtcac caccgacatc ttcgtaatgc
     3301 gcaccgtaga ggtccggata gaacaccgaa ggaacgccat tttcccgcaa caaaattaga
     3361 gcatacgcca gcggtttaaa ccacggttcg accggcgctt cgagggcttg caacggttgg
     3421 gtgtcgtgat tggctacgag ggttacggcg tggaaaggat cggcttccac taatgtaccc
     3481 gtgaaaatct gcgtcatgtc gtagtcgcgt cccatgcgcg atgcttcatg gaatttcatc
     3541 tgcagcggcg catcgaacag catggttttg ccttccacct gatcaatata cgtttgcagt
     3601 ttatcgactt catgcgacca gtactctgcc acaataaaca gcggctttgg cgcaacttcc
     3661 tgtacgtgtt cgatccactc tttataaaac cacgccggaa tatgtttaac cgcatcaaga
     3721 cgaaaaccgt cgcattgtgt ttgttccatc acccagcgcg cccagtattt aatctcttcc
     3781 gtcacggcat gattgcggaa atcgatattc tcacccatca gataatcgaa attaccgagt
     3841 tcatcatcaa cctgatcgtt ccagccttcg ccggtgtagt cgttaacaat tttaaatatg
     3901 ccatcttcat caggattttc gatatggtcg ataccgctaa aacatttgaa gtcccagata
     3961 aactgcgagt attgcccggc acgggcgggg aaggtgtaac gcgtccagcc ttcgcactca
     4021 atgatttctt catcaatttg cgtacggtca tcagcattta cacgctgcac gcgaatagct
     4081 tctttttcat ccgcccccat tttgtggttg actaccacgt ccagtagcac cgcaatgtca
     4141 ttacgtttca gagcgtcaat ggcggccagc agttgtgctt tatcgccata tttagtaggg
     4201 atgctgcctt tctgatcaaa ctcacctaaa tcaaataaat cataggagtc gtagccgacc
     4261 gaatacccgc ccgatgcgcc tttataggcg ggcggcaacc agaccatatt gataccaata
     4321 tcattaaaac cgtcggcgcg ctcggccagt tcaggccaga gcttaccgcc ttccgggtaa
     4381 taccagtgaa aacattgtaa cagcgtggga ttacgcattc ttcgactcca ttcaagggga
     4441 acattagaag cgtagccgta atcggattat tcgcgagcca tcggctcgtt aagattcaaa
     4501 agaggttatc ctgcggagcc agcacaaagc cgccctgatc tccataggca cttaacactg
     4561 atttttgcac cgatgactga ccgaccagtt tcgccagttc atccatacga atctgtaata
     4621 actgctttac cttgctttcg ttgtcgagaa tcaggcgcag catcgggcgg agctgctcct
     4681 gcatcgtggt ggacggggca acctcttcag tcagatgtgc aatctcctgc accgcattca
     4741 catacgccat ttcgctggcg atgagttcgt cccattgttc ttccgttgcc aggcgtaaca
     4801 tgagctggct tttatcgacg agttgttgcc aggcgaaata caaatgcggt gcatggttca
     4861 tcagactggg tcctgaatca aagaagggga gagtaaagac tctttccagg catcggcaat
     4921 attgcgcatt aatgcttcca cttcttcgac tgcggagaca tcgttgcgta aattggcttg
     4981 cagcaagcgc ctgaccatat agctataaag agcaatcaag ttttgggtta gttcgtcttt
     5041 gctctcttca tcaagactca cccgcagccc gttctcaatg atgttgatcg cttttgacaa
     5101 agagacgcct ttgccttgct gattgttgtc ctgcataaac aggcgcgctc taaccagtgc
     5161 gctcagcact ccatcaaata gcatggtgac cagctgttgc t
//
LOCUS       DAJVQG010000205         5114 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJVQG010000205 DAJVQG010000000
VERSION     DAJVQG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.206"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..379
                     /locus_tag="OQM83_003732"
     CDS             <1..379
                     /locus_tag="OQM83_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521878.1"
                     /translation="KKTNIYFDDNFLNIYKRSQDRAPQIISDLKKHNIEIGEKLNDCR
                     KKLNISDVKLTYYWFVTDTGGVDYLDGVLTYNFQGKNRAENFHKQLLQYAYINYSKNQ
                     IPRDFVTSIKNSIYYKIEDCTKF"
     gene            complement(575..2125)
                     /locus_tag="OQM83_003733"
     CDS             complement(575..2125)
                     /locus_tag="OQM83_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCS1521879.1"
                     /translation="MASYHCTVKAGAKGSALKHADYISRSGEYKSYKSREDLEFSSSG
                     NMPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGD
                     RHAYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKK
                     ASIPQTAGERKAALVALRSRWADVQNEHLARHGHESRVDHRSLKEQGINRTPEVHLGP
                     VQAASLNGEQIVAIQERRNAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPEL
                     LLQYRKVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARY
                     LDKLGRNVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHER
                     NEFRNKLQQAESEWDKENAAFKRTEGYKYVGELDRYREREILAAASLENTRQKVAEEV
                     SVARSQMLSLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALCIQRSWARGVSRSN
                     ERDQDMER"
     gene            2297..2743
                     /locus_tag="OQM83_003734"
     CDS             2297..2743
                     /locus_tag="OQM83_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HCS1521880.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKT
                     LSALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVD
                     SKTGKPKFSAAELVGALAGIAELPHNHPKWQEWEKRGKELLTKDSA"
     gene            complement(2937..3872)
                     /locus_tag="OQM83_003735"
     CDS             complement(2937..3872)
                     /locus_tag="OQM83_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCS1521881.1"
                     /translation="MSAALQYFEENLPHRPYHTDELAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDRVGAAIDWSDRNAPAPNITVKNPENGHAHLLYALNLAVRTAPDASV
                     KALKYAAAIERALCEKLDADVNYSGLICKNPFHLEWQVMEWREEAYALDELADYLDLR
                     ASERRSVDKHYGLGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTPESFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLNVHRNTIRNWAMHK"
     gene            4384..>5114
                     /locus_tag="OQM83_003736"
     CDS             4384..>5114
                     /locus_tag="OQM83_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521882.1"
                     /translation="MGKMNINFKHILPLLSSFFISGCDSDGISKVKSFVYYDIDDSMT
                     VGKAFDTRSDCINGTWSEKEDDRGRTIVTYTCDISESGLKIINSAIMQEPEDKAEYSI
                     AQNNNSIKSTNESLEEYKQKTPLVEKTKEVIIDHIRKLNSAFNEDPMIYSKLTISPYL
                     DRMLYLKDHNDNALNEICGGYEYIQSADSYSWRDVSEEYAKESCEKHIYKVYQSFKKN
                     ASPLITKNFPGFYERVPPCENTDEC"
BASE COUNT         1418 a         1244 c         1126 g         1326 t
ORIGIN      
        1 aaaaaaaaca aatatatact ttgatgataa ctttctaaat atctacaaaa gaagccaaga
       61 tagagcacct caaataattt ctgatttaaa aaaacacaat atagaaatag gtgaaaagtt
      121 aaatgattgt aggaaaaaac taaatatctc agatgtcaaa ttaacctatt actggtttgt
      181 aacagatacc ggaggagttg attatttaga tggggtgttg acatataatt ttcagggaaa
      241 gaatagagct gaaaactttc ataaacaact attgcaatat gcatacatta actacagcaa
      301 aaatcaaata cctcgggact ttgtaacatc aataaaaaat agtatttatt ataaaataga
      361 agattgcact aagttttagt ccttccctta caggtggtta cacatataac cacctgcaaa
      421 tatttaacac ctgttaactt cattaatctg aactgtgagt aaatcaaaac gacaattttt
      481 ctcattcaga aaacgtttca cgggacgcgg aattttttcg cgttccgtga aacgttggcg
      541 gggttcgggg cgcagtcccg atattgctac attttcaacg ctccatatct tgatcacgtt
      601 cgttactacg tgataccccc ctggcccagg atcgctgtat acacagtgct ttttcctcct
      661 gttgctgccg ttcctgcgcc atttctgcca gtaactcatg tcgcatttga gctttttcat
      721 ctccagatat actgagttca ggctccagag ataacatctg gctacgggca acggatacct
      781 cttcagcaac cttttgcctg gtgttctcta aagatgccgc cgccagaatc tcgcgttctc
      841 tgtacctgtc cagctcccca acgtacttat atccctcggt acgcttaaaa gcagcgtttt
      901 ctttgtccca ctcggattcg gcttgctgta gcttgttcct gaattcattc ctttcatgtt
      961 cagcggtttc cagcacactt ttttgctcca gaatacgcgc atcaatctcc cttttgtcag
     1021 ccccccgaaa caggccacga atcccattca aagcattgcg ttgttcctgt agctcccgca
     1081 gctccctttg tgcggaaaca acattacgcc ccagtttgtc taaataccgt gccccttcat
     1141 atattcgtct actccattcg gaaagagagt cttttttagc tttcgcattc tgaagcagct
     1201 tatgttcttt gagcgcattg ggattggcat cccctaaacg agcaagcctt gcctctccct
     1261 gaatgaccgt tttcataacc ttgcggtact gaagcaataa ctccggtgaa cgggctgaac
     1321 gaacaggctc aactgccttg attttttgct tttgctcctg ttcctgctga atggcatttg
     1381 cggcatctct ggcggtcttg agttcccgct cggcattcct ccgttcctgg attgcaacaa
     1441 tctgctcccc attcagactg gcggcctgaa cggggccaag atgaacttcc ggcgtgcgat
     1501 tgataccctg ttctttcaga gaacgatgat ctacgcgcga ctcgtgaccg tgtcgcgcaa
     1561 ggtgctcatt ctgcacatct gcccagcggg aacgcagcgc aacaagggcg gcttttcgct
     1621 ctccggctgt ctggggaata ctcgcttttt ttgcgcctcc ccgctctggt gatttgctgt
     1681 tggcacgttt aaaaaattgc tgtggatcgc gttcaatacc atcattaatc cgctcgcaaa
     1741 acatgatatg cgcgtgtggc tgttcctttc cgtcgattgc acctggcgga ttgtggatcg
     1801 cgtactggta ggcatggcga tcgccaagtt ccttttgcac gaaatcttca acaagttcta
     1861 tccgttgttc gcgtgtcagt tcacgcggta acgcgatctc aatttcacgg tatgcagtcc
     1921 cgttttttcg ttcaaattca tctgcggctt tccatagctc tgcgggattt ttcttcgccc
     1981 atgatggcat gttaccggat gacgaaaatt ccagatcctc ccgcgatttg taactcttat
     2041 attcgccgga tctggaaatg taatcagcat gttttaaggc actgcctttt gctccagctt
     2101 tgacagtgca atggtagctt gccatctgcc ttttagccct ccgcaggagc gcacagtttc
     2161 tcgacgaagg agagaaacta ctaagtgcgc atccctgaat taatcatgga tgattttacg
     2221 gtctcacacg caggcaaaca aggtgatatt atgcataata acaactcaca ttaacggcaa
     2281 aagtgcagag gtaagaatga caaatcgtga agagtggtta agtgcaaaaa ttgcctacat
     2341 caacggactg aaatcaccat cagagcagca gagattactt gtgttactgg cagagaaaaa
     2401 aaaccgaaca acgacggatg aaaaaacgtt gtccgcactg atacgggcag aaaaaaccgc
     2461 agagaaagca gccgcagcaa aagccagagt aacggcaatc atagcggcag aacggaaggc
     2521 agccgcaagg gcggaacgaa aggcgcggga tcatgaactc tacaaggccg caggcctcat
     2581 gattgtggca ggactggtgg acagcaaaac cggaaagcca aagtttagtg ctgccgaact
     2641 ggtaggcgca ctcgcaggaa tcgcagagct accgcacaac catccaaaat ggcaggaatg
     2701 ggaaaaacgc gggaaagagc tactcaccaa agacagtgcg taagagcctc cgttcacgtt
     2761 ccagttccct ggtgcttcct cgctcattcg ctcgctacgc tcggtcatga gactgcggcg
     2821 agcacgacca cctcaatcat acggtaagcc gcaccattgc tgggctgaaa ggagaaaacc
     2881 gccggaaagc ccgctgctca aaaagacgcc gaaaaggcgc tgttatctga tataggctat
     2941 ttgtgcattg cccaattacg gatcgtatta cgatgtacat tcagcatcgc cgcaatagct
     3001 ttctgagaat atccattctc cagcatacat agcgccatga aacgcttgtc atcgtaggct
     3061 tcgcctttag cgatgccacc tttccggcct ctcttggcct gtatttctgg tgtgtgcgta
     3121 tcagccacat actgtgcgaa actttccggc gtgaagttcc tgtgcgtgta tttcgcaatg
     3181 ctcttgccaa tagcccgaca ttcaggcgga gaaagcggaa cgggaagcga tgcgttgtac
     3241 atttcgacac gctgaatcac agcatcaagc cactgtgaga atgctggcca gccctgccga
     3301 atcgccctgt aagcccattt gcgcgtcatt tcgaacagat ggcagtttcg ccccagcccg
     3361 taatgtttat cgacgctacg acgctctgag gctctcaaat cgagataatc agccagttca
     3421 tcgagggcgt aggcttcctc gcgccactcc atcacctgcc attccaggtg gaacggattt
     3481 ttgcaaatca gtccgctgta gttcacatct gcgtcaagtt tctcacacag cgcacgttca
     3541 attgcagcgg cgtatttcag cgccttaacc gacgcatcag gcgcggttct cacggcaaga
     3601 ttgagcgcgt agagcaggtg agcgtgcccg ttctcgggat tttttacggt gatgttcggc
     3661 gcgggggcgt tccggtcgct ccagtcaatc gcagctccta cgcggtcaac gtcaaaaacc
     3721 agccagaact gcgcatgagg ctgattttgc tggatgtagc gagcaagcag cgcacgccct
     3781 ttgccggaga tacgaagacc gaaagcgagc tcatccgtgt gatagggacg gtgaggtaaa
     3841 ttttcttcga agtattgaag cgcggcgctc atgtgttacg attccttcca ggttatcgac
     3901 cgacatcgcc cctgtatctt cgtgaaagtc ttgccggata gacacgaatt tcaggggcgt
     3961 ttgtttttca gtcgcttaca ctacccccaa cgacaaaacc tgtcgagtct tcccgcaaac
     4021 ccgatccatt taccataact cccgttgtgc gcccccctta aaacgccctc agtcgctttc
     4081 tgtcgcaaaa aacgcaaaaa gacagggaaa caacaggcca gaccaaaaaa acgcgccaga
     4141 attgttttca gggggctgca taactatgca cgaaagcgat tcatgtccgg tttcactcgt
     4201 tttgaacagc gaacaggtca aaacagcaaa ctttctcatc cagaaaaagg tgaactggtg
     4261 cgaaatcttt tcgaactgcg taagacgttg agcacaaaca tagtaagtcg gtttatcggt
     4321 atgtatggat tccattaaca tatcgcctgt attaatattt cctcgttatt gttagaaata
     4381 aaaatgggaa aaatgaatat taactttaaa catatccttc ctttattgtc gtcatttttt
     4441 atttcaggat gcgactctga tggaataagc aaggtaaagt ctttcgtata ttacgacatt
     4501 gacgactcaa tgactgttgg taaagccttc gatacaagga gtgattgcat caatggcaca
     4561 tggagtgaaa aagaggatga tcgaggaaga accatcgtca catatacctg tgatattagt
     4621 gaaagtggcc taaaaataat aaattcagcc attatgcaag aacctgaaga taaggctgaa
     4681 tattctatag cacaaaataa caacagcata aaatcaacca atgaatcact agaggaatac
     4741 aaacagaaaa cgcctctggt ggaaaaaaca aaagaggtca taatagatca tataagaaag
     4801 ctaaacagtg cttttaatga agatccaatg atatatagca aactcacaat tagcccttat
     4861 ttagacagaa tgctatatct aaaggaccat aatgacaatg cgctaaatga aatttgtggt
     4921 gggtacgaat acattcaatc tgccgatagt tacagttggc gtgatgtaag tgaagagtat
     4981 gcaaaagaat catgcgaaaa gcatatatat aaagtatatc aatcgtttaa gaaaaatgca
     5041 tcaccattaa taactaaaaa cttccccggt ttctacgaac gtgtcccccc ctgtgagaac
     5101 acagatgaat gcat
//
LOCUS       DAJVQG010000206         5040 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJVQG010000206 DAJVQG010000000
VERSION     DAJVQG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.207"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            357..982
                     /gene="gstB"
                     /locus_tag="OQM83_003737"
                     /pseudo
     CDS             357..982
                     /gene="gstB"
                     /locus_tag="OQM83_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(979..2094)
                     /gene="yliI"
                     /locus_tag="OQM83_003738"
     CDS             complement(979..2094)
                     /gene="yliI"
                     /locus_tag="OQM83_003738"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HCS1521883.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKHYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(2205..2588)
                     /gene="bssR"
                     /locus_tag="OQM83_003739"
     CDS             complement(2205..2588)
                     /gene="bssR"
                     /locus_tag="OQM83_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCS1521884.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            2801..4126
                     /gene="rimO"
                     /locus_tag="OQM83_003740"
     CDS             2801..4126
                     /gene="rimO"
                     /locus_tag="OQM83_003740"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCS1521885.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4173..5040)
                     /locus_tag="OQM83_003741"
                     /pseudo
     CDS             complement(4173..5040)
                     /locus_tag="OQM83_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
BASE COUNT         1304 a         1275 c         1207 g         1254 t
ORIGIN      
        1 acgctcggcg cttcaacggt ttgttccgcc gcgaatgccg ttggggcaaa aaggaataaa
       61 aatgcagaac ccgctgcaag accacgaagg agagaggagt attgcgtcat aaatgccacc
      121 cgagtatcca ttcaaaaaaa atacgttata tcgccgtatg ataagttcgg tgattaataa
      181 cgcacacaca aacttaaaga aaccgtaagt tggtaaagtt tttaaagttt atgcaatatc
      241 ctgccgcttc gctgcttctg gtgtcatttt tttgccagct gttgcttaaa agttaacttt
      301 acttcaccat cggagtcctg ttcagcctgt ggctgcctgt cggaaaaggg gtgagtatga
      361 ttacgctgtg gggtcggaat aattcaacta acgtaaaaaa gtattgctga cgctcgaaga
      421 actggaacta ccttatgagc aaattctcgc gggccgtgag tttgggataa atcacgatgc
      481 tgattttctg gcgatgaacc ctaacgggct ggtgccgttg ttgcgtgacg acgaaagtga
      541 tctcattctt tgggaatcaa acgccattgt ccgctatctg gcggcacagt acgggcaaaa
      601 acgcctgtgg atcgactcac cggcacgtcg tgcggaagcg gaaaaatgga tggactgggc
      661 aaaccagacg ctcagtaatg ctcatcgcgg gatcctgatg ggattagtca gaacaccacc
      721 ggaagagcgc gatcaggccg ccattgatgc cagttgcaaa gagtgcgacg ccctgtttgc
      781 cctgctcgat gcggaactgg caaaagtaaa atggttctct ggcgacgagt ttggtgtggg
      841 cgatatcgct atcgcaccgt tcatctacaa tttgtttaac gttggcctga cctggacacc
      901 gcgtccaaat ctgcaacgct ggtatcagca actcactgaa cgccccgcag tgcgcaaagt
      961 ggtgatgatt cccgttagct aattgcgtgg gctaacttta agtaattccc cactggactc
     1021 gtcggtgaga acgtataaat aaccgtcggg tccagtgcga acatcacgaa ttcgctgccc
     1081 tctgtccgtt aaaatacggc catcttctgt cactttgtcg ccgttgacgc tcatcacaat
     1141 gacatcttta tctttcagcg cgccaataaa taatttttgc tgccactggg ggaatttatc
     1201 gctgttatag aaggccatgc cgctcacagc gggcgaatct ttccagtaaa aaacaggttg
     1261 ctcggtcccg gcgacgatct cccctttcgc ttccggtatc ttaaagcctg aatagttgat
     1321 tccccaggtt gccagcggcc agccgtagtg tttgcctttt tgcgggatat taatttcatc
     1381 accaccgcgc gggccatgtt cattcagcca cagtgcatta ctccacggat tcatcgccat
     1441 tccttgcgga ttacgaatgc cataagacca gatctcggcg cgcgcaccgg attcctttat
     1501 aaaaggatta tcatccggga tttcgccctg gtcggtcaga cgcaccagtt tgccctgtaa
     1561 tttatccaga tcctgcgccg tcgggcgctg attgttttcg cccagagcaa taaaaagata
     1621 acctttaccg tcgaatacca gccgcccgcc aaaatggttg ccggtagaca gttttggcat
     1681 ctggcggaag acggtgcgga agtcggtcac ttttgagaga tcatcactta agcggccata
     1741 acccacagca gttccggctt tgccatcatc gccaacttcg gaataactta accagatgcg
     1801 gcgagactga gcaaaatcag gcgctaaaac cacgtccagc aggccgccct gcccgtgcgc
     1861 ccaaacgtcc ggaactccgg aaagcggcgc agataatcct tttcctgctt gccagtgacg
     1921 caactcgccg ccgcgcagag tgattaacat accgtgatta tcgggtaaaa aggccagtgc
     1981 ccagggatgg tcgagtttgt cttgcagtac ttcgacattt accgttgcag gagccgccca
     2041 gagagcggaa gaaagacaaa taaggggcac aaggaaaaag gattgtcgat gcataacgcc
     2101 tccggattga ctctggctta aagcgtagtc agtggaggag ataacaaatt catttttaca
     2161 aaaacttaaa catgaagggg gagacgcttt ctccccctta gttttcaggc cttctcaagc
     2221 atggcgtgct tctgcaggct ctggatactc agcgttaagc tcatcagaca attttcaagc
     2281 ttatcggcgt tgacggtaat aacagtcggg caatcatggt gcccactcat caaacatact
     2341 gcggctgtcg ctaatgcttc ttcagcatga tgaagagcac tccactcttc ctgatccaga
     2401 tgaagattca accgcagcga tttatcgtgc agttcgcgat tcagtttaaa aaagttatct
     2461 cgtagatgat tgctttcgct gacggacatg tatccttttg ccttcctcag ttgcacgtat
     2521 gcagcgaggt caacgcgtgc gtcgatcaac cggttcagca gatcgattcg ctgtctgtca
     2581 acgaacatac ttcgatcctc ctctttccag ccttgttgcg attaaatagt atggctattt
     2641 tttgagcaaa atgctgtcca ggatcaattc ttcacgccaa attaacaaaa tttacatttc
     2701 cttcgcctcc gccagcgaat gagattgcga tacaaatttt atccagaact ctgtagaatc
     2761 cctgccctga cctttcctgt aactgaatat tttttaagct atgagcaaag taactcccca
     2821 gccgaaaatc ggctttgttt cccttggctg tccgaaaaac cttgtcgatt cagagcgtat
     2881 tctcaccgaa ctacgcactg aaggttatga cgtggtaccg agctatgacg atgcggacat
     2941 ggtgatcgtc aacacctgcg gctttattga cagcgcggta caagaatcac tggaagccat
     3001 tggtgaagcg ttgaatgaaa acggcaaggt aattgtgacc ggttgtctgg gggcaaaaga
     3061 agatcagatc cgcgaagtcc acccgaaagt gctggaaatc accgggcctc atagctatga
     3121 gcaggttctg gagcacgttc atcactacgt gccaaaaccg aaacacaacc cattcctgag
     3181 cctggtgcca gaacaaggtg tgaaactgac accgcgtcat tatgcctatc tgaaaatttc
     3241 ggaaggctgt aatcaccgct gcaccttctg cattattccg tctatgcgcg gcgacctggt
     3301 gagccgtccg attggcgaag tgttaagtga agcgaaacgt ctggtagatg cgggcgttaa
     3361 agagattctg gtgatctcgc aggatacttc cgcctatggc gttgatgtta aacatcgtac
     3421 tggcttccac aacggcgagc cggtaaaaac cagcatggtc agcctgtgcg aacagttatc
     3481 gaaactgggg atctggacac gtctgcacta cgtttaccct tatccgcatg tggacgacgt
     3541 catcccactg atggcagaag gcaaaatcct gccgtatctg gacattccgt tgcagcacgc
     3601 cagcccgcgc attctcaaac tgatgaagcg tccgggttct gtagatcgcc aactggcgcg
     3661 catcaaacag tggcgcgaaa tctgcccgga actgacccta cgctcaacct ttattgtcgg
     3721 cttccctagc gagacggaag aagatttcca gatgctactc gacttcctga aagaagcgcg
     3781 tctggatcgc gttggctgct ttaaatacag cccggttgaa ggtgcagacg ccaatgccct
     3841 gcctgaccag gttccggaag aagtgaaaga agaacgctgg aaccgtttca tgcagctgca
     3901 gcagcagatt tccgccgagc gcctgcaaga gaaagtgggc cgtgaaattc tggtgattat
     3961 cgacgaagtg gacgaagaag gcgcgattgg tcgcagcatg gcagatgcac cggaaatcga
     4021 cggcgcggtt tatctcaatg gtgaaaccaa cgttaagccg ggtgatatcc tgcgtgtgaa
     4081 agtcgagcac gccgatgagt acgatttgtg gggtagccgg gtttaattaa ttcaggccgg
     4141 gcaaaatcat ttgtccgccc tgcgaataat gattactgcc agctaatctg agccctgccg
     4201 tttttcttac tcttatacag ccgctcatcc gccagcattt tcaagcgttc gacggtggta
     4261 cattcatttg aatgcgctac accgatactg gtacttaaat taatgcttcc cacctggaaa
     4321 tgaacgattt cttttagaat cgatagcaac tcttccagcg ttccacccgt ataaatcacc
     4381 gcaaattcgt caccgccgaa gcggtagatt ttacctttat ctaccgggat ctgtgattta
     4441 aggattttca ccactttcat caacacttca tcgcccagca aatgaccgaa ggtgtcgtta
     4501 atgtttttga atttatcgac gtcaaacagg cacaaataac cgcctttttc cggcagcgct
     4561 tttaaatctt cgtcaaagga ttttctgccc attgctttgg tcaggtcgtc caggaacgcc
     4621 gagttataca tactgtagag ttttttgaca tcggcagccg aaaagaaaag cccgccatcc
     4681 agtacgcgtt caaaatggtt attaatgatg gcttctttct ccacaatacc acgattaatc
     4741 actctgcgta ccagatagag gcaactggta cccgatatca ctgccaccaa tgcggcaaag
     4801 aaaataatca atgtacagac ccgcaaataa acatcttttt tatacgcaaa tacgtacaat
     4861 ccggtcggca aataggcgaa gtgctccatc gcttccgaaa cggtgaagat ggcgttttta
     4921 gcattatatt catcaccggt ttaccgttct taaatagaga gaacatcagg ttctcgctgt
     4981 ccatcgccag cgattttgac agtaaagaaa aacgctccag accaagcaaa aatatcagac
//
LOCUS       DAJVQG010000207         5033 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJVQG010000207 DAJVQG010000000
VERSION     DAJVQG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.208"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            74..189
                     /gene="rrf"
                     /locus_tag="OQM83_003742"
     rRNA            74..189
                     /gene="rrf"
                     /locus_tag="OQM83_003742"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(307..750)
                     /locus_tag="OQM83_003743"
     CDS             complement(307..750)
                     /locus_tag="OQM83_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCS1521886.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            907..1836
                     /gene="metA"
                     /locus_tag="OQM83_003744"
     CDS             907..1836
                     /gene="metA"
                     /locus_tag="OQM83_003744"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCS1521887.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            2105..3706
                     /gene="aceB"
                     /locus_tag="OQM83_003745"
                     /pseudo
     CDS             2105..3706
                     /gene="aceB"
                     /locus_tag="OQM83_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3736..5027
                     /gene="aceA"
                     /locus_tag="OQM83_003746"
                     /pseudo
     CDS             3736..5027
                     /gene="aceA"
                     /locus_tag="OQM83_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1260 a         1320 c         1284 g         1169 t
ORIGIN      
        1 cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat
       61 aaaacagaat ttgcctggcg gccgtagcgc ggtggtccca cctgacccca tgccgaactc
      121 agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa
      181 ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt gcattggcgc
      241 agaaaaaaat gccgggtgcg acgctggcgc gtcttctcca gcctgggcag gcacggcagg
      301 ggcagcttat tcctccacat acgccagatt cagcaacgga tacggtttcc ccaaatcgtc
      361 cacctcagag cgtcccgtaa ccttaaaacc caccttctta tagaacccaa ccgcctgctc
      421 attttgctca ttaacgttgg ttgtcagttc cggtgccatc gagagcgcat gctccaccag
      481 catccgaccc acgccgcagc cgcacacatc aggatcgata aacagcgcat ccatatgctg
      541 cccacttaac aacataaacc caaccggctg atcccgttca ttaaccgcga cccacaacgg
      601 cgcttccggc aggaaggaac gaactagcac ctccagctcg gcccgatact ctgctgataa
      661 aaaatcgtgt gtggcatcga cagaacgaca ccaaatcgca acgagttcct ccccttcctc
      721 atgccgtgag cggcgaatac taataaccat tttctctcct tttcgtcatt cttatattct
      781 aacgtagcct tttccttgaa actttctcac cttcaacatg caggctcgac attggcaaat
      841 tttctggtta tcttcagcta tctggatgtc taaacgtata agcgtatgta gtgaggtaat
      901 caggttatgc cgattcgtgt gccggacgag ctacccgccg tcaatttctt gcgtgaagaa
      961 aacgtctttg tgatgacaac ttctcgtgcg tctggtcagg aaattcgtcc acttaaggtt
     1021 ctgatcctta acctgatgcc gaagaagatt gaaactgaaa atcagtttct gcgcctgctt
     1081 tcaaactcac ctttgcaggt cgatattcag ctgttgcgca tcgattcccg tgaatcgcgc
     1141 aacacgcccg cagagcatct gaacaacttc tactgtaact ttgaagatat tcaggaacaa
     1201 aactttgacg gtctgatagt taccggcgcg ccgctgggcc tggtggagtt taatgatgtc
     1261 gcttactggc cgcagatcaa acaggtgctg gagtggtcga aagatcacgt cacctcgacg
     1321 ctgtttgtct gctgggcggt acaggccgcg ctcaatatcc tctacggcat tcctaagcaa
     1381 actcgcaccg acaaactctc tggcgtttac gagcatcata ttctccatcc tcatgcgctt
     1441 ctgacgcgtg gctttgatga ttcattcctg gcaccgcatt cgcgctatgc tgactttccg
     1501 gcagcgttga ttcgtgatta caccgatctg gaaattctgg cagagacgga agaaggggat
     1561 gcatatctgt ttgccagtaa agataagcgc attgcctttg tgacgggcca tcccgaatat
     1621 gatgcgcaaa cgctggcgca ggaatatttc cgcgatgtgg aagccggact agacccggat
     1681 gtaccgtata actatttccc gcacaatgat ccgcaaaaca aaccgcgcgc gagctggcgt
     1741 agtcacggta atttgctgtt taccaactgg ctcaactatt acgtctacca gatcacgcca
     1801 tacgatctac ggcacatgaa tccaacgctg gattaatctt ctgtgatagt cgatcgttaa
     1861 gcgattcagc accttacctc aggcaccttc gggtgccttt tttatttccg aaacgcacct
     1921 cagcaagtga ataaatttta ttcatattgt tatcaataag ttatcaagta tttttaatta
     1981 aaatggaaat tgtttttgat tttgcatttt aaatgagtag tcttagttgt gctgaacgaa
     2041 aagcgcacaa cgatccttcg ttcacagtgg ggaagttttc ggatccatga cgaggagctg
     2101 cacgatgact gaacaggcaa caacaaccga tgaactggct ttcacaaggc cgtatggcga
     2161 gcaggagaag caaattctta ctgccgaagc ggtagaattt ctgactgagc tggtgacgca
     2221 ttttacgcca caacgcaata aacttctggc agcgcgcatt cagcagcagc aagatattga
     2281 taacggaacg ttgcctgatt ttatttcgga aacagcttcc attcgcgatg ctgattggaa
     2341 aattcgcggg attcctgcgg acttagaaga ccgccgcgta gagataactg gcccggtaga
     2401 gcgcaagatg gtgatcaacg cgctcaacgc caatgtgaaa gtctttatgg ccgatttcga
     2461 agattcactg gccccggact ggaacaaagt gatcgacggg caaattaacc tgcgtgatgc
     2521 agttaacggc accatcagct ataccaatga agcaggcaaa atttatcagc tcaagcccaa
     2581 tccagcggtt ttgatttgtc gggtacgcgg tctacacttg ccggaaaaac atgtcacctg
     2641 gcgtggtgag gcaatccccg gtagcctgtt tgattttgcg ctctatttct tccacaacta
     2701 tcaggcactg ttggcaaagg gcagcggtcc ctatttctat ctgccgaaaa cccagtcctg
     2761 gcaggaagcg gcctggtgga gcgaagtctt cagctatgca gaagatcgct ttaatctgcc
     2821 gcgcggcgcc atcaaggcga cgttgctgat tgaaacgctg cccgccgtgt tccagatgga
     2881 cgaaatcctt cacgcgctgc gcgaccatat tgttggtctg aactgcggtc gctgggatta
     2941 catcttcagc tatatcaaaa cgttgaaaaa ctatcccgat cgtgtcctgc cagacagaca
     3001 ggccgtgacg atggataaac cattcctgaa tgcttactca cgcctgctga ttaaaacctg
     3061 ccataaacgc ggtgcttttg cgatgggcgg catggcagcg tttattccga gcaaagatga
     3121 agagcgtaat aaccaggtgc tcaacaaagt aaaagcggat aaagcgctgg aagtcaataa
     3181 cggtcacgat ggcacatgga tcgctcaccc aggccttgcg gacacggcaa tggcggtatt
     3241 caacgacatt ctcggctccc gtaaaaatca gcttgaagta ttgcgcgaac aagacgcgcc
     3301 gattactgcc gatcagctgc tggcaccttg tgacggtgaa cgcaccgaag aaggtatgcg
     3361 cgccaacatt cgcgtggctg tgcagtacat cgaagcgtgg atctccggca acggctgcgt
     3421 gccgatttat ggcctgatgg aagatgcggc gacggctgaa atttcccgta cctcgatctg
     3481 gcagtggatc catcatcaaa aaacgttgag caatggcaaa ccggtgacca aagccttgtt
     3541 ccgctagatg ctgggcgaag agatgaaagt cattgccagc gaactgggcg aagaacgttt
     3601 ctcccagggc cgttttgacg atgccgcacg cttgatggaa cagatcacca cttccgatga
     3661 gttaattgat ttcctgaccc tgccaggcta ccgcctgtta gcgtaaacca ccacataact
     3721 atggagcatc tgcacatgaa aacccgtaca caacaaattg aagaattaca gaaagagtgg
     3781 actcaaccgc gttgggaagg cattactcgc ccatacagtg cggaagatgt ggtgaaatta
     3841 cgcggttcag tcaatcctga atgcacgctg gcgcaactgg gcgcagcgaa aatgtggcgt
     3901 ctgctgcacg gtgagtcgaa aaaaggctac atcaacagcc tcggtgcact gactggcggt
     3961 caggcgctgc aacaggcgaa agcgggtatt gaagcagtct atctgtcggg atggcaggta
     4021 gcggcggacg ctaacctggc ggccagcatg tatccggatc agtcgctcta tccggcaaac
     4081 tcggtgccag ctgtggtgga gcggatcaac aacaccttcc gtcgtgccga tcagatccaa
     4141 tggtccgcgg gcattgagcc gggcgatccg cgctatgtcg attacttcct gccgatcgtt
     4201 gccgatgcgg aagccggttt tggcggtgtc ctgaatgcct ttgaactgat gaaagcgatg
     4261 attgaagccg gtgcagcggc agttcacttc gaagatcagc tggcgtcagt gaagaaatgc
     4321 ggtcacatgg gcggcaaagt tttggtgcca actcaagaag ctattcagaa actggtcgcg
     4381 gcgcgtctgg cagctgacgt gacgggcgtt ccaaccctac tggttgctcg taccgatgcc
     4441 gatgcagcgg atctgatcac ctccgattgc gacccgtatg acagcgaatt tattaccggc
     4501 gagcgtacca gtgaaggctt cttccgtact catgcgggca ttgagcaagc gatcagccgt
     4561 ggcctggcgt atgcgccata tgctgacctg gtctggtgtg aaacctccac gccggatctg
     4621 gaactggcgc gtcgctttgc acaagctatc cacgcgaaat atccgggcaa actgctggct
     4681 tataactgct cgccgtcgtt caactggcag aaaaacctcg acgacaaaac tattgccagc
     4741 ttccagcagc agctgtcgga tatgggctac aagttccagt tcatcaccct ggcaggtatc
     4801 cacagcatgt ggttcaacat gtttgacctg gcaaacgcct atgcctaggg cgagggtatg
     4861 aagcactacg ttgagaaagt gcagcagccg gaatttgccg ctataccttc gtatctcacc
     4921 agcaggaagt gggtacaggt tacttcgata aagtgacgac tattattcag ggcggcacgt
     4981 cttcagtcac cgcgctgacc ggctccactg aagaatcgca gttctaagca acc
//
LOCUS       DAJVQG010000208         4996 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJVQG010000208 DAJVQG010000000
VERSION     DAJVQG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.209"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="OQM83_003747"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="OQM83_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCS1521888.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="OQM83_003748"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="OQM83_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HCS1521889.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            833..1993
                     /gene="pheA"
                     /locus_tag="OQM83_003749"
     CDS             833..1993
                     /gene="pheA"
                     /locus_tag="OQM83_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCS1521890.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEIFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="OQM83_003750"
     CDS             complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="OQM83_003750"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCS1521891.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="OQM83_003751"
     CDS             complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="OQM83_003751"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCS1521892.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4447..4812
                     /locus_tag="OQM83_003752"
     CDS             4447..4812
                     /locus_tag="OQM83_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCS1521893.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
BASE COUNT         1377 a         1262 c         1127 g         1230 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggaacggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgga
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aaccgggggg ccttttttat tgataacaaa aaaggcaaca ctatgacatc
      841 ggaaaatcca ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
      901 attactggca gagcggcgcg aactggccgt cgaggtggga aaagccaaac tgctctcgca
      961 tcgcccggta cgagatattg atcgtgaacg cgatttactg gaaagattaa ttacgctcgg
     1021 taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga
     1081 ttccgtatta actcagcagg ctttgctcca gcaacatctc aataaaatta atccgcactc
     1141 agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta
     1201 tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt
     1261 taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc
     1321 cggtgccata aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga
     1381 gatgacgtta actatcgacc attgtttgtt ggtctccggc acgactgatt tatccaccat
     1441 taatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta
     1501 tcctcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca
     1561 ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt tgtacggttt
     1621 gcaggtactg gagcgtattg aagcgaatca gcaacaaaac ttcacccgat ttgtggtgtt
     1681 ggcacgtaaa gccattaaca tgtctgacca ggttccggcg aaaacgacgt tgttaatggc
     1741 gaccgggcaa caagccggtg cgctggttga agcgttgctg gtgctgcgca accacaatct
     1801 gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatatttta
     1861 tctggatatt caggccaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg
     1921 ggaaattacc cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt
     1981 tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact
     2041 ggcggttgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt
     2101 aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct
     2161 gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat
     2221 tacgctctga cgacataata atgtcggcat aaagctgcgg atcctgagca aacagtcgcc
     2281 cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct
     2341 gaacattttc ttctgccaga tgcagcccat aagcaaaagt agcaaagtgg cgcagagcct
     2401 gaataaacgc catattctga tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc
     2461 agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga
     2521 ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta
     2581 ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat
     2641 caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt
     2701 ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc
     2761 agtcatgttg ctccagaatc cgcacctgat aacccgagag tgtcagcatc ttctcgaaca
     2821 ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg
     2881 ttttaaatcc tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat
     2941 cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca
     3001 tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc acctcgccca
     3061 cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta tcgacttcat
     3121 caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga
     3181 gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc
     3241 cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctca
     3301 gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgattg
     3361 ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta
     3421 ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc
     3481 tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg
     3541 atgacatgcc cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta
     3601 atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga
     3661 ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg
     3721 tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg
     3781 tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc
     3841 tcaagcagca atttacgcgc gatctgcagc ccggcttcta catcaaaaga gccatccata
     3901 tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg
     3961 cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa tcgacgagca
     4021 tattccagag cagtttccgg atcatgaatg gaacaaggac cacaaactac cagcagacga
     4081 ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct
     4141 tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg
     4201 tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct gtttatgctc
     4261 gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt
     4321 caatccatat ttcgtacatt tttctttaca cgaacaattt gctcgcgctt ttgatgctta
     4381 tttcgtacaa ataaatttac acctccttta aaaacaatcc gctatgcttt gaaaaaggag
     4441 aaagaaatga tgaaaaagtt tatcgccccc ttattggctt tactggttag cggatgtcag
     4501 attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga tgtcggtatg
     4561 gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga ttcacaggcg
     4621 gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg ccagaccgat
     4681 tttacttatg cccggggact ttccggtaaa agctttcctg ccagctgtaa caatgttgaa
     4741 agtgccagcc aactgcatga agtatggcaa aaaggggctg atgaaaatgc cagcacaata
     4801 cgattaaatt aattaaaacc ccacaaataa attaaacata agattttact cattgtgttt
     4861 tatttctcac attgatgacg atcgcatgaa aaaatgatat tttaatgagc attctgaaaa
     4921 aatatcgatg catttcgagc gaagatggtg gggatccctg aatgcgtttt tctcaccgac
     4981 tgttcttact tctgat
//
LOCUS       DAJVQG010000209         4961 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJVQG010000209 DAJVQG010000000
VERSION     DAJVQG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.210"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            427..2712
                     /gene="nrdA"
                     /locus_tag="OQM83_003753"
     CDS             427..2712
                     /gene="nrdA"
                     /locus_tag="OQM83_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCS1521894.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2946..4076
                     /gene="nrdB"
                     /locus_tag="OQM83_003754"
     CDS             2946..4076
                     /gene="nrdB"
                     /locus_tag="OQM83_003754"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCS1521895.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4076..4330
                     /gene="yfaE"
                     /locus_tag="OQM83_003755"
     CDS             4076..4330
                     /gene="yfaE"
                     /locus_tag="OQM83_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCS1521896.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4407..>4961)
                     /gene="inaA"
                     /locus_tag="OQM83_003756"
     CDS             complement(4407..>4961)
                     /gene="inaA"
                     /locus_tag="OQM83_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCS1521897.1"
                     /translation="NYRRNGMSGVQCVERNGKKLYVKRMTHHLFHSVRYPFGRPTIVR
                     EVAVIKELERAGVIVPKIVFGEAVKIEGEWRALLVTEDMAGFISIADWYARHAVSPYS
                     DEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIYVKTEGKAEAGFLDLEKSRRRLRRN
                     KAINHDFRQLENIWSQSPKRTGSR"
BASE COUNT         1226 a         1334 c         1227 g         1174 t
ORIGIN      
        1 catttcgaca tcaacaatcg cttacattcg cttatatatt gaccacaact gatacatcag
       61 attatgtgat gactcgtgct tagatcaatt tttgcaatca ttaacaaaaa gattaataaa
      121 ccatctatat caatttatcc aacctattct gccgttcaag aaatcgccga acagttattt
      181 ttaacaaatt tttctcttcc cattgacttt cccggacacc ttgtctgacc taaggtgcgc
      241 gaaagccact ttttccttcc tgagttatcc acaaagttat gcacttgcaa gagggtcatt
      301 ttcacactat cttgcagtga atcccaaaca taccccctat atatagtgtt ctaagcagct
      361 tcccttacta caggtagtct gcatgaaact attgcggaaa gaattccaaa aacaggtacg
      421 acatacatga atcagaatct gctggtgaca aagcgcgacg gtagcacaga gcgcatcaat
      481 ctcgacaaaa tccatcgcgt tctggattgg gcggcagaag gactgcataa cgtttcgatt
      541 tcccaggtcg agctgcgctc ccacattcag ttttatgacg gtatcaagac ctctgacatc
      601 cacgaaacca ttatcaaggc tgccgcagac ctgatctccc gtgatgcgcc ggattatcag
      661 tatctcgccg cgcgcctggc gatcttccac ctgcgtaaaa aagcctacgg ccagtttgag
      721 ccgcctgcgc tgtacgacca cgtggtgaaa atggtcgaga tgggcaaata cgataatcat
      781 ctgctggaag actacacgga agaagagttc aagcagatgg acacgtttat cgatcacgac
      841 cgtgatatga ccttctctta tgctgccgtt aagcagctgg aaggcaaata tctggtacag
      901 aaccgcgtga ccggcgaaat ctatgagagc gcccagttcc tttatattct ggttgccgcg
      961 tgcctgttct cgaactaccc gcgtgaaacg cgcctgcaat atgtgaagcg tttttacgac
     1021 gcggtttcca catttaaaat ttcgctgccg acgccaatca tgtccggcgt gcgtaccccg
     1081 actcgtcagt tcagctcctg cgtactgatc gagtgcggtg acagcctgga ttccatcaac
     1141 gccacctcca gcgcgattgt taaatacgtt tcccagcgtg ccgggatcgg catcaacgcc
     1201 gggcgtattc gtgcgctggg tagcccgatt cgcggtggtg aagcgttcca taccggctgc
     1261 attccgttct acaaacattt ccagacagcg gtgaaatcct gctctcaggg cggtgtgcgc
     1321 ggcggtgcgg caacgctgtt ctacccgatg tggcatctgg aagtggaaag cctgctggtg
     1381 ttgaaaaaca accgtggtgt ggaaggcaac cgcgtgcgtc atatggacta cggggtacaa
     1441 atcaacaaac tgatgtatac ccgtctgctg aaaggtgaag atatcaccct gttcagcccg
     1501 tccgacgtac cggggctgta cgacgcgttc ttcgccgatc aggaagagtt tgaacgtctg
     1561 tataccaaat atgagaaaga cgacagcatc cgcaagcagc gtgtgaaagc cgttgagctg
     1621 ttctcgctga tgatgcagga acgtgcgtct accggtcgta tctatattca gaacgttgac
     1681 cactgcaata cccatagccc gtttgatccg gccatcgcgc cagtgcgtca gtctaacctg
     1741 tgcctggaga tagccctgcc gaccaaaccg ctgaacgacg tcaacgacga gaacggtgaa
     1801 atcgcgctgt gtacgctgtc tgctttcaac ctgggcgcaa ttaataacct ggatgaactg
     1861 gaagagctgg caattctggc ggttcgtgca cttgacgcgc tgctggatta tcaggattac
     1921 ccgatcccgg ccgccaaacg tggagcgatg ggtcgtcgta cgctgggtat tggtgtgatc
     1981 aacttcgctt actacctggc gaagcacggt aaacgctact ccgacggcag cgccaacaac
     2041 ctgacgcata aaaccttcga agccattcag tattacctgc tgaaagcctc taatgagctg
     2101 gcgaaagagc aaggcgcgtg cccgtggttt aacgaaacca cttacgcgaa agggatcctg
     2161 ccgatcgata cctataagaa agatctggat accatcgcta atgagccgct gcattacgac
     2221 tgggaagcat tgcgtgaatc aatcaaaacg cacggcctgc gtaactctac gctttctgcc
     2281 ctgatgccgt ccgagacctc ttcgcagatc tctaacgcca ctaacggtat tgaaccgccg
     2341 cgcggttacg tcagcatcaa agcgtcgaaa gacggtattt tgcgccaggt ggtgccggac
     2401 tacgagcacc tgcacgacgc ctatgagctg ctgtgggaaa tgccgggtaa cgatggctac
     2461 ctgcaactgg taggtatcat gcagaaattt atcgatcagt cgatctctgc taacaccaac
     2521 tacgatccgt cacgtttccc gtcaggaaaa gtgccgatgc agcagttgct gaaagacctg
     2581 ctcaccgcct acaaattcgg ggtcaaaacg ctgtattatc agaacacccg tgacggcgct
     2641 gaagacgcgc aggacgatct ggtaccgtca atccaggacg atggctgcga aagcggcgca
     2701 tgtaagatct gatattgaga tgccggatgc ggcgtaaacg ccttatccgg cctacggctc
     2761 ggtttgtagg cctgataaga cgcgccagcg tcgcatcagg ctccgggtgc cggatgcagc
     2821 gtgaacgcct tatccggcct acggctcgga tttgtaggcc tgataagacg cgccagcgtc
     2881 gcatcaggca caggatgcgg cgtaaaatgc cttatccggc attaaactcc caacaggaca
     2941 cactcatggc atataccacc ttttcacaga cgaaaaatga tcagctcaaa gaaccgatgt
     3001 tctttggtca gccggtcaac gtggctcgct acgatcagca aaaatatgac atcttcgaaa
     3061 agctgatcga aaagcagctc tctttcttct ggcgtccgga agaagttgac gtctcccgcg
     3121 accgtataga ttaccaggcg ctgccggagc acgaaaaaca catctttatc agcaacctga
     3181 aatatcagac gctgctggat tccattcagg gtcgtagccc gaacgtggcg ctattgccgc
     3241 ttatttctat tccggaactg gaaacctggg tcgaaacctg ggcgttctca gaaacgattc
     3301 attcccgttc ctatactcat atcattcgta atatcgttaa cgatccgtct gttgtgtttg
     3361 acgatatcgt caccaacgag cagatccaga aacgtgcgga agggatctcc agctattacg
     3421 atgaactgat cgaaatgacc agctactggc accttctggg cgaaggcacc cacaccgtta
     3481 acggtaaaac cgtaaccgtt agcctgcgcg agctgaagaa aaaactgtat ctctgcctga
     3541 tgagcgttaa cgcgctggaa gcgatccgct tctacgtcag ctttgcttgt tcctttgcct
     3601 ttgcagaacg cgaactgatg gaaggtaacg ccaagattat tcgcctgatt gcccgcgatg
     3661 aagccctgca cctgaccggt actcagcata tgctgaatct gctgcgcagc ggcgcggatg
     3721 atccggagat ggcggaaatt gccgaagaat gtaagcagga gtgctatgac ctgtttgtcc
     3781 aggcggcaca gcaagagaaa gactgggcgg attatctgtt ccgtgacggt tcgatgattg
     3841 gtctgaataa agatattctc tgccagtacg ttgaatacat caccaatatc cgcatgcagg
     3901 cagtcggttt ggatctgccg ttccagacgc gctccaaccc gatcccgtgg atcaacacct
     3961 ggctggtgtc tgataacgtg caggttgctc cgcaggaagt ggaagtcagt tcttatctgg
     4021 tcgggcagat tgacgcggaa gtggacaccg acgatttgag taacttccag ctctgatggc
     4081 ccgcgttacc ctgcgcatca ctggcacaca actgctgtgc caggatgaac acccttccct
     4141 tctggcggcg ctggaatccc acaatgttgc ggttgagtac cagtgccgcg aaggttactg
     4201 cggctcctgt cgcacccgac tggtcgcagg tcaggtggac tggattgccg aaccgttagc
     4261 ctttattcag ccgggggaaa ttttgccctg ttgttgccgg gcaaaaggcg atattgaaat
     4321 cgagatgtga attggttgta gtgccagata caacgcttat acgtcttatc tggcctacaa
     4381 cgattacatg gcgtagtaat acgctttcac ctgctcccag tccgctttgg ggattggctc
     4441 cagatatttt ccagctggcg gaagtcatga ttaatcgctt tattgcgacg caagcggcga
     4501 cgacttttct ccaggtcaag aaaaccagct tctgctttac cttctgtttt cacatagata
     4561 tggcgaacat aacaacagcc atgctgacga ttaacgctat gcattttctt aaacgccagc
     4621 gccactgctt tcaacatggc ttgccgtact tcgtcagaat agggcgatac tgcatgccgg
     4681 gcataccagt cagcaatgct gatgaacccc gccatatctt cagtcaccag caacgcgcgc
     4741 cattcaccct caattttcac cgcttcacca aaaacgatct tcggcacgat gacaccggcc
     4801 cgttccagtt ctttaattac cgcaacctca cggacaatcg ttggtcggcc gaacggataa
     4861 cgtacggaat gaaacagatg atgcgtcatg cgctttacat acagcttttt gccgttgcgc
     4921 tcgacgcatt gcaccccgct cataccatta cggcgatagt t
//
LOCUS       DAJVQG010000210         4800 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJVQG010000210 DAJVQG010000000
VERSION     DAJVQG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.212"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(276..1319)
                     /locus_tag="OQM83_003757"
     CDS             complement(276..1319)
                     /locus_tag="OQM83_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCS1521898.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            complement(1731..2915)
                     /locus_tag="OQM83_003758"
     CDS             complement(1731..2915)
                     /locus_tag="OQM83_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1521899.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            complement(3215..3309)
                     /locus_tag="OQM83_003759"
     tRNA            complement(3215..3309)
                     /locus_tag="OQM83_003759"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3273..3275),aa:Sec,seq:tca)
     gene            3602..>4800
                     /locus_tag="OQM83_003760"
     CDS             3602..>4800
                     /locus_tag="OQM83_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HCS1521900.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
BASE COUNT         1253 a         1055 c         1089 g         1403 t
ORIGIN      
        1 ataccagttc aaccgaaaac gccagaggag gggattaccc gctggcaggg taaatctgtg
       61 gtttttgatg ccagctataa cgtgtttgac gataaagtat tacgaaagaa ctttaatgag
      121 ttcatggagt aattcgttaa agtaatttac aacgctatcc ttttgcaaag gaaactttca
      181 gaaaataacc ccagggtaat cacctgggga aggaacacag tgttattatt ggataataaa
      241 ttattacttg ttttaaagtt acactaatgc aatatctact gatactggat gcttgaatgt
      301 ttcatccctg ctgcggaaaa gggcaatatc cagggccaga tatattcacc gttcatattt
      361 ctgcatttct gtacaatttc ctcagaaagt gtcttgtcaa ttcttttcca caggtgctcc
      421 tgctgtctta aaatactttc gcatatttcg aaatcatctt cctgaatcag catttcggcg
      481 catttaatga tttctgatcg ttcatgcagc acggaggaat ggttatagag ccagtttaca
      541 aacagcaatg cttccggata ttgtccccat aatggtgagt atgttgcata gtaatcttct
      601 gccgtatcca gagagccgga gaagtcatgc cacacagcaa gtggaaatgg atgcagattt
      661 ccgcacagca tttctgcgat gtaagtgttt gtggcaaagc catggcgaaa agctgtggcg
      721 gccttaacat gttccccgtt cagaaaatgc aggagaccga gttcatacca gtaaggtgaa
      781 aattcatcag catgctcttt cagtacactg aaggctcgct catgatcgcc ggtgcgtaac
      841 agctctgaac ccaacaacca gcgagccccc tgattatctt caggattgta tgccagtatt
      901 ttatcagtga gcataaccgc atcctgatgg cgctgtaagt gcacatttgc gagaattgct
      961 gcataaagcg cgcgcagata aggccggttt tcgggatgca tccagattat ttcaccacag
     1021 aaactttccg ggatgatacg attacctgcc gccagtcctt taagagaggc gttcagtgcc
     1081 ttacgcgggg cattctgttc cagaaatgca tatgccagat gagcgtgtat atcaataaag
     1141 tccggctccc gttgagcaag gcgtcggagt ttgttgatgt aactcgtctc gctgatttca
     1201 tcagcgtcgt atgcatcaag cacttcttca aactccccca gcaatgggcc aaaccaggta
     1261 tccggaaata cgaaataacc cttgttatcg tgcacttcaa agcataatct atcgttcatt
     1321 tctgagcctg taaaacatgt ggtgatatag tctgagagca taaaagttga gattctgtgt
     1381 atcaagtcct gtcttggcac cgaatgaata ttgcttacga agccccttca tgctttcctt
     1441 gagaacaata attttatctt tatgtgaatc tcatttttta taatgatgtc agatgattga
     1501 aatgctaaat tatatctgaa tcaacttttc ctttgctaaa ggtatcgaaa taactgcaag
     1561 gctgtatttt gtaattgcca atttaaaaat gtcaatttgt ttttaattat gtttttgtaa
     1621 taagtttgaa gacgcaaaaa aaataatata ataacaaaaa tcctccagcc ttgtaggcca
     1681 gaggataaaa tacttaattg aggaaaatat cagggaaaag tatttctgac ttaactttcc
     1741 cggctggaga gtgaatcaat gtaatcagcg taccattgca tcatttcacg gcggccatcg
     1801 agatacaggg catgattata ggtacccctg atggaatttt tatccacatg agctaactgc
     1861 atttcaatcc aggcggaatt aaagccttgt tcatgcagaa ttgtactcat ggtgtgtctg
     1921 aaaccgtgcc ctgtgagtct tcccttatat ccaagtaatt caattacttg gttaatgctt
     1981 tccttactaa ttggtttgcg gggatcattt ctaccgataa agactagggg atgatgtttt
     2041 gagattggtt caagctgctt aaatagcata atgacctgct cagagagcgg cacaatatga
     2101 ggtcgcttca tcttcattac ttctgccggg atctcccaca attttgtctc aaaatcgata
     2161 tcttcccagc gtgcaaaacg cagttcttgt gttcgtacgc cggttaacat aatgatctga
     2221 gtagctgtct tagtgattat acttccggta taacccgcca gatcgttgag gaagtgggga
     2281 agttcattag cagtaagaaa gggaaaatgt acttttttcg gtgtagctaa agcactggca
     2341 agatcggggg ctgggttata gtctgcccgg ccagtaacaa ttgcatagcg gaacacctca
     2401 ccacagcgct ggcggacctt gcgcattttc tccagagctc cacgtttttc cattttacgc
     2461 agtgcttcca gtaactccat tggtttgatt tcggcaatag ggcgcttgcc aatgtaggga
     2521 aaaatgtctt tttcaaaggt atcaatgatt tcatcacgat agcgtaatga ccaccgatca
     2581 gcttttgact ggtgccattc gcgagcaata gcctcgaacg tatttttcag actcatttgc
     2641 tgcgcgattt tttcttcctt ttttgcctcc cctggatcgc ctccagcagc gaggagtttc
     2701 ctggcttcac tgcgtttctc gcgagcatcg gctagcgtta catcggggta tacgccaata
     2761 gaaagcatct tttctttacc agcaaagcgg tacttcatgc gccagtattt agaaccatta
     2821 gtgttaatca gtagatacat accgccgcca tcagcaagct tatagggttt gtctttgggt
     2881 tttgcggttt tcactttgat atcggttaat gccatgacat cctccggctt ttgatgaatt
     2941 catgttcctg agggggattt ctgccagtaa ctaacctgat acccccaata caccccttgt
     3001 ataacaaaga tattgttaaa agccgttgtt atgtatcaca tttttcctat tatgggggtg
     3061 cttgtatttc aataggaaat aacaccccct aaagtacccc caaaagaaac ttgatatggg
     3121 gtgaagtagg ttgacctcag gatacaaata aatactgata tacaagggat tagaagagat
     3181 ttattgacat tgggagagtt cagaagaggt aaactggcgg aagatcacag gagtcgaacc
     3241 tgcccgggac cgctggcggc cccaactgga tttgaagtcc agccgcctca ccggagacga
     3301 cgatcttccg cgcctcgatt gctacatgga ggcggggcgc attatagcta cttccttgag
     3361 tttctacatc ccccagatca gttttttccg cctaatacac gcagcgtatt atcacgaaag
     3421 cattataact catataaaac ccatatttta caatgatata tgagcggaat taacgctacc
     3481 acgatcacat aaaacaagaa tcataaatta ataaccagat atcggaatat tcgctctcac
     3541 agggatggtt tacaaaatgc gttatcggtc tcagcacccc tatagcatca aggaaaagca
     3601 gatgaagagt gaagtgttgt ccgttaaaga gaaaattggt tatggcatgg gagacgccgc
     3661 cagccacatt attttcgata acgtaatgtt atatatgatg ttcttttata ccgatatttt
     3721 tggcattcct gccggatttg tcggaaccat gtttttggtc gctcgtgcac tggatgcgat
     3781 ttccgatcct tgcatggggt tgttggccga tcgaacgcgc tctcgctggg gtaaatttcg
     3841 tccgtgggta ctgtttggcg cactgccatt cgggatcgtc tgtgtactgg cctatagcac
     3901 gccagatctc agtatgaacg gcaaaatgat ctatgcagca attacttaca ccctacttac
     3961 cttactttat accgtcgtca atatccctta ctgcgcattg ggtggtgtaa tcaccaatga
     4021 cccgactcag cgtatctcgc tgcaatcctg gcgttttgtg ctggcgaccg cgggaggcat
     4081 gctttctact gttctgatga tgccactggt taatttaatt ggcggtgata ataaaccact
     4141 cggtttccag ggcggtatcg cggtcctttc cgtggtggca ttcatgatgc tggcattttg
     4201 tttcttcacc actaaagaac gcgttgaagc accacctaca acaacgtcta tgcgggaaga
     4261 tttacgtgat atctggcaaa acgaccagtg gcggattgtc ggtttactaa ccattttcaa
     4321 tatcctggcg gtgtgcgtac gcggtggggc gatgatgtat tacgtcacat ggattttggg
     4381 cacgccggaa gtgtttgtcg cttttctcac cacttattgc gtgggtaacc tgattggttc
     4441 cgcactggca aaacctctga ccgactggaa atgtaaagtc actatcttct ggtggacgaa
     4501 cgccctgctg gcagtgatta gcctcgcgat gttctttgtt cccatgcagg ccagcatcac
     4561 tatgtttgtc ttcatcttcg tgattggtgt gttgcatcaa ctggtgacac ctatccagtg
     4621 ggtaatgatg tccgataccg tcgactacgg cgagtggtgc aatggtaaac gcctgaccgg
     4681 gatcagtttt gctggcacgc tgtttgtgct caaactgggg ttggccttcg gcggcgctct
     4741 tatcggctgg atgctggctt atggcggata tgatgcggca gaaaaagcgc agaacagcgc
//
LOCUS       DAJVQG010000211         4708 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJVQG010000211 DAJVQG010000000
VERSION     DAJVQG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.214"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(670..744)
                     /locus_tag="OQM83_003761"
     tRNA            complement(670..744)
                     /locus_tag="OQM83_003761"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(709..711),aa:Gly,seq:tcc)
     gene            complement(748..823)
                     /locus_tag="OQM83_003762"
     tRNA            complement(748..823)
                     /locus_tag="OQM83_003762"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Thr,seq:tgt)
     gene            complement(826..902)
                     /locus_tag="OQM83_003763"
     tRNA            complement(826..902)
                     /locus_tag="OQM83_003763"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(866..868),aa:Arg,seq:tct)
     gene            complement(904..980)
                     /locus_tag="OQM83_003764"
     tRNA            complement(904..980)
                     /locus_tag="OQM83_003764"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(944..946),aa:Ile,seq:cat)
     gene            complement(1170..1733)
                     /locus_tag="OQM83_003765"
     CDS             complement(1170..1733)
                     /locus_tag="OQM83_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCS1521901.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1837..2055)
                     /locus_tag="OQM83_003766"
     CDS             complement(1837..2055)
                     /locus_tag="OQM83_003766"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521902.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(2214..2600)
                     /locus_tag="OQM83_003767"
     CDS             complement(2214..2600)
                     /locus_tag="OQM83_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS1521903.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(2590..2961)
                     /locus_tag="OQM83_003768"
     CDS             complement(2590..2961)
                     /locus_tag="OQM83_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCS1521904.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(2961..3218)
                     /locus_tag="OQM83_003769"
     CDS             complement(2961..3218)
                     /locus_tag="OQM83_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521905.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(3215..4606)
                     /locus_tag="OQM83_003770"
     CDS             complement(3215..4606)
                     /locus_tag="OQM83_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCS1521906.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQATGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYS
                     NTRTDSSKMERFF"
     gene            complement(4603..>4708)
                     /locus_tag="OQM83_003771"
     CDS             complement(4603..>4708)
                     /locus_tag="OQM83_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1521907.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
BASE COUNT         1161 a         1285 c         1051 g         1211 t
ORIGIN      
        1 cgacagacct gtgctatcgg aaagaaaaaa ggccatcagc aaaactctga tggcctgaat
       61 cacctttacc aatattgtat gaaaaaacac gcacgactaa ttgacaataa ttttcatttc
      121 cattaaaaaa gatcgcgtag cattcttaat tcatgaggaa ctttacccgc cagcaatctg
      181 agtagcgaaa gctgttcatc cccaatgttt ttgctggcgg gtcctttttt ctacggtcct
      241 cctccccgga ggggggcatt tcattatttt tctaatcgtt actggtgaga aggcggaaga
      301 aaaagccagt tcttcgcccc taaataacag catgattcca gtccatgcaa aatctgattt
      361 ccagctcaca cataccgagc gctgtacaaa aaaccgccag ccaaggcagt taagagtgtg
      421 ttcccggggt ttgcttagaa tattttgtaa gttgtccgaa gagatattta caacaccaga
      481 atgatgaatc atcagtcccc tgccagaggt tcactacgta acctgaccga caatgcacca
      541 ttctggtgct gaaaaaagag cactggaact gcaacaaaac aacatcacag aacaggagat
      601 ggtaaggagt cagacattcc cacacaatat tgtgtcagtg caaataacaa ccttcgtctc
      661 aaatctcgct ggagcgggca gcgggaatcg aacccgcatc atcagcttgg aaggctgagg
      721 taatagccat tatacgatgc ccgcatatgg tgccgactac cggaatcgaa ctggtgacct
      781 actgattaca agtcagttgc tctgcctgct gagctaagtc ggcgctggcc caccaccgag
      841 gactcgaacc tcgcaccgtc aacttagaag gttgatgctc tatccggatg agctagtggt
      901 ggttggtggc ccttgctgga cttgaaccag cgacctggcg attatgagtc gctcgctctg
      961 accaactgag ctaaagggcc ggaggcagaa taataaccat atgtcatcac atctgcaaac
     1021 tcatctgacc accagcacgt ttaacgtcct gtgccgtttt tcaggtataa aaaaacccgc
     1081 tcatcggcgg gtttaagctg tgtggcgtag taaccactct taacaggata ttcaactttt
     1141 tacgatcgta aagttttcgg aaaaaatttt taaaaccgca tcaaccgctc caccagttgc
     1201 tctttacggg caacgatcca cccgtgttgc tccagataaa atttaaaccg ttccagagta
     1261 cataccatcg catcggcggg taccttttcc gtgaactcaa cctgaccgtg tttatcgaag
     1321 tggctcagta atgcgcatcc atcattttcg atagatgtgt tttgtgccgc tggtggctgt
     1381 ttctggttga aataacagtc ttccagtttt tcgaacactt cccacgcctg atcggtttcg
     1441 agcattttgg cgtgacgggc tgcgccacgt tctgtccaga ggatgaggga gcgaacgtta
     1501 cgggcaattt tcacagaaac tcttaaagag ttttcgttct tcaactctcg taacgcatca
     1561 ccttctatca ggaaaaaatg tttaccttct ataaaccgtt ctttgtttcg attgaaattg
     1621 tttgtcaaac gctggcgctc tgttccgtac aaatcagcca gcagctctgt tgtcatgaca
     1681 ggaagatggt tatgcgtaag tgacgggagt ttttcaatag acgtgcgatt catagacatg
     1741 tcctttcgaa tattttgatt tacccctttt gagagggtga ccgggcgctc aaaaccgtcg
     1801 aaagtcggcg ggcgtatttc cattgctggt attgtattag ccgcacaccc ggtcataaac
     1861 caagaattct ggacacaaaa aaaccacatg gctatcgggt gtggataccg ctttcgaggt
     1921 gttttgagca ccgcggggaa atgtaaactg caaacctgaa actgtcaaca ccgtcaggtt
     1981 ccaatcatcg ggtggtgaga cgcacagggt tggaactacc gggaaaccga ccggcgagct
     2041 tttcagctcc cccatgcgcc ccaccataat tcagatgtgc gcgtgcatac gacaataaaa
     2101 aacacgctcg cggcgtgtgt ctgtcgcggt ctctatccgg ggttccaatc ccgacgccag
     2161 attttgctgg cgcgtgagga atatagcccc ggacaatgtg tctggtcaag cacctacata
     2221 attcgttcta cgtatctgtc catctccagc cggatatcaa gcatcatcaa catgccatca
     2281 ataacccctt ccgctttctg caggcgcttg ccaatacagg tatccgaaca cccatgtttc
     2341 cgtgccagac tcataaaagt cattccacct acgtaataat ccaccagcaa atcgtgcaaa
     2401 tcctgatttt tcttgttcaa ccgggccata cagccacaaa ttatcattgc atcatcatca
     2461 gaacactgag ggcgtgattt cactttcgac ggaattaacc ctttaaaacc agcagcgatc
     2521 gacgcccagg atacatcttc atgagtgttt gcagcccaag ccccccaccg ttccataacc
     2581 tgctgaatat cacgcgccat cgttatcacc tgtgatttca taaatcttca cgcccagtcg
     2641 cccaccagga acgagctgac cgcgcacaat attgatttca tcaaactgct cgtcgtctat
     2701 gagtagtccg gcatgcgtca gcgcatccag tggtgctttc aggatattgt ccaggtcacg
     2761 acggcgctta tccggtggct ctgcaataat ctttatcgcc agccttccgg acaggtttaa
     2821 tttcagccgc tgctggcgaa caattagcgt cacatcacgg cgataacgct caccaacttt
     2881 tgatacaaaa tatgtgctgc cacgacgtcg ccagtaagta ttcaccgtcg gcggataagg
     2941 taaaataaac tcatggcgca tcagcgcagc acctcctgca ccattttttc aaactttcca
     3001 actttggttt ccagctctgc cacacaatca acgagctcat ccaccgcttt ttgtgcgcga
     3061 tgtttcgcct gcatcagttc cctgagcgct ggcaccatat cccgacgaat ggcatctttt
     3121 gttacacctg ttttttccag ttgttccgcc tgtcgcaaca tttcctgcgc gtgtttacgc
     3181 aattgttcag aggtaaaagt cattgtctgg ttgttcaaaa gaaacgctcc atcttactgc
     3241 tgtcggtacg tgtattgctg tatctgcgcg gctggggctg ccgcattggg gtggaaagaa
     3301 cctgtgcgct ttcctggtcc acaggcagaa aatgtccgtt ataaaaacgc cggtaaatcg
     3361 tccccagaga accgttacgt tgtttcgtga tattaatttc tgctatgcct gtagcctgtg
     3421 tatccgggtt gtacacttca tccctgtaaa gcatcagaat gatgtctgca tccgcctcta
     3481 tttctccgga atttttcagg tctgagttca tgggacgttt attgggtctg gactccacac
     3541 cgcgggagag ctggctcagc gcaatcaacg gaaaaccacc ggattttgcc aggcctttaa
     3601 gcccctttga gatttcaccc acggcaaggt catgacgccc cgtggttcgg gtttttatca
     3661 gcccgagata atcaaccacc accagcgccg tttccggatg tttaatcaga tggtgtttcg
     3721 ttgttgcgca tatctcatca atggtcaggt tcgcctggtc caccatccag atattgcgcc
     3781 cggtcatccg ccccacccct tgtgagaaac gcgcccagtc ttcatcttca aagtgagcca
     3841 cagatttcag gcgtgatact ggcatccctc cagccgcaga caccatacgt tcaccaatct
     3901 ggatgttcgc catctccatg gtgaacagaa gcacaccatg cccctgctca gtcaccttgt
     3961 cgatgatgtc cagcgcaagt tcggttttcc ccatcgaagg acgggcggca atgaatacca
     4021 ggtctccggg ctccataccg cctgtttttg cgtccagttc atcaataccg gtcatcaacg
     4081 tcctggattt ctccagcccc tgattccggc attcaacacg ctcaaccact tccggaagca
     4141 catcatcaat atgtaccggc tgaatgacgc cctttcctgt cgacagtgtg accatcatgt
     4201 tctgcgcatc cttcagggca tcttcagctg cttcacaggt atgcgcatca cgtaatttct
     4261 gcagcgcctc attcagtgtt ttttctgcat cgcgcagtgc ggcattgcgc cgcaacgctg
     4321 caacatagtg ctccagtgaa gacttcaccc aggttttacg cccggtatca gtaatcaccg
     4381 gggcaagttc cggcatctca ttacacaaca gcacggggtc aatcactcct gaaacacggg
     4441 cctgtctgca gatgcctgtg tagatatccc gatacgctcg tacagaaaaa atgtccgccg
     4501 gtagtgtgat cagaatatcc atcacttcat gatctgcccc acgcagaaag aatgcgccaa
     4561 tgacagcgcc ttccaggtca tcattacgcc agactggtgt tgtcatgcag ccacacctct
     4621 gatacaagaa cggtagctgg gccagttgaa cgacaaccag ttgcgccccc cgtctgtgat
     4681 cctgtcggca atgcgggggc tgatgaac
//
LOCUS       DAJVQG010000212         4506 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJVQG010000212 DAJVQG010000000
VERSION     DAJVQG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.215"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(9..953)
                     /gene="mhpB"
                     /locus_tag="OQM83_003772"
     CDS             complement(9..953)
                     /gene="mhpB"
                     /locus_tag="OQM83_003772"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HCS1521908.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(955..2619)
                     /locus_tag="OQM83_003773"
     CDS             complement(955..2619)
                     /locus_tag="OQM83_003773"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HCS1521909.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            2661..3494
                     /gene="mhpR"
                     /locus_tag="OQM83_003774"
                     /pseudo
     CDS             2661..3494
                     /gene="mhpR"
                     /locus_tag="OQM83_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
     gene            3571..>4506
                     /gene="lacI"
                     /locus_tag="OQM83_003775"
     CDS             3571..>4506
                     /gene="lacI"
                     /locus_tag="OQM83_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HCS1521910.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQA"
BASE COUNT         1036 a         1292 c         1240 g          938 t
ORIGIN      
        1 ctgcatattc agttctctgt tctggcgctt aacgagccaa atccggcaat ccactccggg
       61 attgggcggt aataacgccc ttcgctacgc cagttgccaa atgcagaaat agcggcaaaa
      121 gcggcgaccc aggttttgat ttcatgtgtc gactttccgg caatggcgga aagctcttcg
      181 ttactgacgg catccagttc ctgtatgcgt ccctgctcca gcaaagtcat gaactggtta
      241 tcccaaatcg ggttgagcgg atgcagagtt ctctgatcct caacaaactt ctcagcggcg
      301 ctaatcaccc tttgctgacg caattcgcgc tcactggcgg gtaaatcttt cccgctcccc
      361 aacagacggt cgcgcatatg ggcatcggct ttcgccagtt cgggcaccgg cggctgatgg
      421 gaaagcccac cggaacccag gaacagcacg cgtttattga gagtgctggt gaaacgtcca
      481 atggcttcac ccaacatgcg ggtacgctgg aaaccgggca gcggcgtggc gacaccgttg
      541 atgaacacag gcagaactgg caccttatcc agcccaccga gcaggaactc cagcggctgg
      601 gcgaacccgt ggtccacctg catacagtaa gaaacggcaa gatcgatccc gctcttcatg
      661 acagcatgcg cacaggcctc cgccagctcc acaggcacgg gcagctctcc tgccgcactg
      721 ccgaaatcac caattgccgt cgctccaacg cctaaacaga acggtggcat cacgtcatag
      781 aaaaagccgt tgtagtgatc tggcgcaaac agcaccacca gttcagggga gaaagccgca
      841 atacgctcgc gggcgctggc aatcacgcca ttgacctcat cgagtacctc ttgcgccggg
      901 tcgacatacc ccaccaacgg cgagtgggaa agacagtgaa gataagcgtg catatcaggc
      961 taccttttcg acagaaacgt cggcatcagg gcgggtcagc gtcatcaccg acgccagttt
     1021 attcagcgta ttgcccaggg tttgcggaat ggcggtggcg gcaacaaagc gatccgggcg
     1081 catcaccacc agcgaagcat tgtgttgcgc gaaccagcta cgcagacgac cttgcgtatc
     1141 gcccacgcgt agcacgccgt cgtggttatc ctgtgcggta tgaatttgca cttccggcac
     1201 cacctgaatg aagcgtgtgc ccaacgcgcg ccactgctgg atttgctcgt cgctcatccc
     1261 ccacagtgga ttgcatcccc agccaattac cgcgaagttc gcgccgatcg cgttatcgag
     1321 cagcgtcacg tcgccgtttt ccagcgtgac tttcggctga ataaacatct tgccgaccgg
     1381 agagtgcttc gcttcgccct cacgcaccag cgcaccgccg taatattgcg gcatcggctt
     1441 gaagcgcatt tcgaggaagt agcgttttac tggcggcaga taattcaaca gccaggaaac
     1501 gccgtcacgt aacgtaccct gccagcgttt cggcggagcc agcacgttgc ccgccgtcac
     1561 ggacaggtca atcatcgctt tggcgtgatc gcgacgttct tgttgatagg tatcgagcag
     1621 cgcatcgcgg gctttcccct ggataaccaa cgccagtttc caggcgaggt taaaggcgtc
     1681 gcgcatacca ctgttatagc cctgcccctg ccacaccggc atgatgtgcg cggcatcgcc
     1741 cgccagcagt acgcgatcaa tacggaaacg ttgcgccagt cgcgcgttat gggtgtagac
     1801 acgctggcga atcaattcaa cattgtccgg attaggcagc actttgctta acagcttgcg
     1861 catattttgc ggctcacgca gctgctcttc ggtttctccc ggcatcacca taaattcaaa
     1921 gcgacgtacc gcatgaggca gcgcggcaga aacatacggg cgcaccggat cgcaacacaa
     1981 atagatatgc ggcgtactta acggatcgtt ggcgatatct accacaatcc actgatttgg
     2041 cgcagtttta ccttcaaacg gcacattcag ggtgcgacga acaaaacttg ccccaccatc
     2101 acaggccacc agccactggg ctttgatcgt ttcccgctgc ccttctgccg ttttcaggtg
     2161 caaggtcact tcgtcatctt gctggctgaa ggcctccagc tcgcgggaaa acaagcagcg
     2221 cacattcgga aaacgcgaca acccttccag catcaccgca tcgacctgtg gctgaataaa
     2281 ggcgttacgg cgcggccagc caaattcatc ggtcattggc tgaatatcag caaaacagcg
     2341 gccttttggg gtgagaaaac gcatcgcgtg ccacggtgta gtgtgcggca gaacattctc
     2401 gaccaggccg accgactgca tggtgcgcag cgcctcgtca tcaataccaa tcgcacgcgg
     2461 gtagtcgatc aacttatcga gtttctccac caccagcacg tcaatgccca tctgaccgag
     2521 atagttcgcc atcatcagcc caaccggacc ggcaccagcg atcgccacct gaacgctatg
     2581 gttaacagca ggctggatgt cagggtgttg tattgccatt tctgcacaac gttgttttaa
     2641 ctatagaaat gtcaattaat atgcagaaca atgagcagac ggaatacaaa accgtgcgcg
     2701 gcttaacccg cggtctaatg ttattaaata tgttaaataa acttgatggc ggtgccagcg
     2761 tcgggctgct ggcggaactc agcggcctgc atcgcaccac tgtgcggcga ctgctggaga
     2821 cgctgcagga agagggatat gtccgccgta gcccctccga tgacagtttt cgtctgacca
     2881 tcaaagtgcg gcaattaagc gaaggatttc gtgacgaaca gtggatttct gcactggcgg
     2941 ccccgctgct gggcgatctg ttgcgcgaag tggtatgacc aacagatgtg tccaccctgg
     3001 atgttgatgc aatggtggta cgcgaaacca cccaccgttt cagccgctta tcctttcacc
     3061 gggcaatggt cgggcgacgt ttaccgctcc tgaaaaccgc ctcgggcctg acctggctgg
     3121 ccttttgccc ggaacaagaa cgcaaggaat taatcgaaat gttagccgcc cgccccggtg
     3181 atgactatca actggcacga gaaccgttaa agctgcaagc cattctggcg cgcgcgcgca
     3241 aagagggtta cggacagaac taccgcggct gggatcagga ggagaagatc gcctctatcg
     3301 ccgtaccgct gcgcagtgaa caacgggtga ttggctgtct gaatctggtg tatatggcga
     3361 gcgcaatgac cattgaacag gcagcggaaa agcatcttcc ggcgctacaa cgggtagcaa
     3421 aacagatcga agaaggggtt gaatcgcagg ctattctggt ggccggacgg cgaagcggcg
     3481 tgcatttacg ttgacaccat cgaatggcgc aaaacctttc gtggtatggc atgatagcgc
     3541 ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgctata cgatgtcgca
     3601 gagtatgccg gtgtctctta tcagaccgtt tctcgcgtgg tgaaccaggc cagccacgtt
     3661 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac
     3721 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt
     3781 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg
     3841 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg
     3901 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac
     3961 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc
     4021 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc
     4081 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt
     4141 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt
     4201 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg
     4261 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg
     4321 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta
     4381 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa
     4441 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
     4501 caggcg
//
LOCUS       DAJVQG010000213         4406 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJVQG010000213 DAJVQG010000000
VERSION     DAJVQG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.216"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            6..81
                     /locus_tag="OQM83_003776"
     tRNA            6..81
                     /locus_tag="OQM83_003776"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="OQM83_003777"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="OQM83_003777"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="OQM83_003778"
     CDS             complement(299..1057)
                     /locus_tag="OQM83_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCS1521911.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTKAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="OQM83_003779"
     CDS             complement(1065..2168)
                     /locus_tag="OQM83_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCS1521912.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /locus_tag="OQM83_003780"
                     /pseudo
     CDS             complement(2178..3359)
                     /locus_tag="OQM83_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3427..>4406)
                     /locus_tag="OQM83_003781"
     CDS             complement(3427..>4406)
                     /locus_tag="OQM83_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1521913.1"
                     /translation="AVANQAHAGATLDAVQKKGFVQCGISDGLPGFSYADADGKFSGI
                     DVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLSRNTTWTSSRDAGMGMA
                     FTGVTDYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELNVADYFKANNMKYTPVT
                     FDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVLPEVISKEPLGPVVRRG
                     DDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAHLLGKEGDYGKDLKLDN
                     KWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGIQYAPPVR"
BASE COUNT         1202 a         1165 c         1052 g          987 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt agggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccttcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgc agcttgctcc actatttccc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgcgtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggct
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctactac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccagt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gtgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca atgccagtga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatatt ggtgccttct
     1501 gccataaaca aaggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccagaccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgattg acacaaggcc aatcagtaat
     1861 gccaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatca ctcgcagtgc ctggggaata atgacctagc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggca
     3301 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccagcttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcatgg cgaaaagcgt
     3721 ccagcgtaca atcgagaacc attcatcatc gccacgacga actaccggcc caagcggttc
     3781 tttagagata acctccggta agacaatcca ttcagctggg ttgcttaatt tgatgcgcag
     3841 ggcatacagt tgcgactgat ccgaggccag cgtatcgcag cgaccagatt ccagtgcctt
     3901 cgctgattcg tcagagcgat cgaaagtcac cggtgtgtac ttcatattgt ttgccttgaa
     3961 atagtcggcg acgttgagtt cggtatctgt acccgcctga atacagacgg tagcgccatc
     4021 cagttctttc gcgcttttta gccccgcttt atcgtgcgtc aggaagccaa tgccgtcgta
     4081 atcagtgacg ccagtaaatg ccattcccat cccggcatcg cgagatgaag tccaggtcgt
     4141 attacgggag agcaaatcca cctcccctga ctgtaaagcg gtgaagcgtt cttttgcagt
     4201 gagcggggta tatttcactt tcgtgtcgtc accaaataca gcagcggcaa caccacgaca
     4261 aacatctaca tcaatacctg aaaacttacc gtctgcatcg gcataagaga acccaggtaa
     4321 tccatcactg atcccgcatt gcacaaaacc ttttttctgt acggcatcaa gcgtcgcgcc
     4381 agcatgcgcc tgatttgcaa cggcaa
//
LOCUS       DAJVQG010000214         4404 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJVQG010000214 DAJVQG010000000
VERSION     DAJVQG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.217"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(139..3654)
                     /locus_tag="OQM83_003782"
     CDS             complement(139..3654)
                     /locus_tag="OQM83_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS1521914.1"
                     /translation="MDKNALGFTSYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4106..>4404)
                     /locus_tag="OQM83_003783"
     CDS             complement(4106..>4404)
                     /locus_tag="OQM83_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1521915.1"
                     /translation="VNKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQM
                     SHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRDKGISPFEYAKINNPLK"
BASE COUNT         1141 a         1127 c          905 g         1231 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagatgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg ataccttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca ttaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcgtcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaggagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aatcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcacct gcagaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc ttaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt tggcgtcttt ccgttcaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggt aaaccctaaa gcatttttat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aaccaccata tggacaacta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcggtat caaatgtact
     3781 ggttgtacaa ggttcagaac acaagcacat tcaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa tgactgcctg tgttggacaa ggataccaga
     3901 acagccgaaa acaccggggg ttattacctg ctctcatgga aaccagatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacat gacgaaggga ctttaatgga agccggagta
     4021 acttgccaga acatcaatct tcgtcatcat ttacaaagaa aaataatcag ttaaagcagt
     4081 ccgggcaatc agcccggact gcttactatt ttaatggatt gttaatcttt gcatattcaa
     4141 acgggctgat acccttatct ctgttcgcat ccagaaaatc tgcccaccac tgtagcatca
     4201 gtcgacgttc ttccagatgt tctgctttat ggatatacgc agcacgtaca ccattacgct
     4261 cctgatggct catctgacgt tccacagcat cgcgggacca caaacctgat tcaatcaatg
     4321 cactgcacgc catcgcccgg aatccatggc cacagacatc ctgggttgtg tcatacccca
     4381 tcaccctcag ggctttattt acag
//
LOCUS       DAJVQG010000215         4400 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJVQG010000215 DAJVQG010000000
VERSION     DAJVQG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.218"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(11..1948)
                     /locus_tag="OQM83_003784"
     CDS             complement(11..1948)
                     /locus_tag="OQM83_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCS1521916.1"
                     /translation="MNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSRSGI
                     LALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPPFYD
                     TLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGDLLI
                     HEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKSNHH
                     GALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFRLAI
                     IQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNENDP
                     GIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYPLFA
                     ALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDYPTS
                     VLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVPATI
                     LAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAEVLP
                     SVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAHSAD
                     MTFRVKPESLILTIFFLGLIP"
     gene            2359..3066
                     /locus_tag="OQM83_003785"
     CDS             2359..3066
                     /locus_tag="OQM83_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCS1521917.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3172..3247
                     /locus_tag="OQM83_003786"
     tRNA            3172..3247
                     /locus_tag="OQM83_003786"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3205..3207,aa:Phe,seq:gaa)
     gene            3445..>4400
                     /locus_tag="OQM83_003787"
     CDS             3445..>4400
                     /locus_tag="OQM83_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCS1521918.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1147 a         1160 c         1056 g         1037 t
ORIGIN      
        1 tacgtcgccc tcagggaatt aaacctaaaa agaaaatcgt caaaattaga gattccggtt
       61 tgacacggaa agtcatatca gcgctatgcg catcctgggg gttcatcacc actgacggga
      121 aactctgctg gcggaacatc gctttttgta ggtctttgac gtcgaaactg acatacagat
      181 cgtgcatctc ctgacacaac tggcgcaggg tgtagtcgcg atagcgcacc ggatatttgt
      241 tataaacgct cggcaacacc tcagccagcg gcgagtcatc ctcaatatgc tgttcaaatt
      301 gcgccagcat cgccaccagt tgtgccagct tctcgtggct ttccgccgga gttaataaga
      361 acagaatgga gttgagatcg cacttctccg gcacaatgcc gttctcacgc agatagtgcg
      421 ccagaatcgt cgccggaacg ccaaagtcgc tatattcgcc ggtttcggca ttgatacctg
      481 gtgtagtgag taacagcttg cacggatcaa caaaatactg atccgcggca tatccttcaa
      541 agccgtgcca cttcgccccc ggctcaaaac tgaaaaaacg gcggtcgctg gctaacacag
      601 atgtcggata atcctgccac aatttgccat caacaacggg cgggataaac gggcggaaca
      661 gcttacagcg cgcaagaata gccttgcgcg actcaatacc caactcaacg cactcagccc
      721 acagccgacg cccactctcc ccttcatgaa ttttggcgtt aacatccagt gcagcaaaca
      781 gcggatagaa agggctggta gaagcatgga gcataaaggc gttattcaac cgcttatgcg
      841 ggcaaaaacg cgcctgtccg cggatatggt tatctttttt atggatctgc gacgtctgtg
      901 agaatcccgc ctgctgtttg tgcaccgact gagtcacaaa gatccccgga tcgttttcgt
      961 taagttctaa tagcagcggc gagctatccg ccatcatcgg gataaactgt tcgtaaccga
     1021 cccacgcgga atcaaacaga atgtaatcac acagatgccc aacggtatcg atcacctgac
     1081 gggcgttata gacagttccg tcataggttc ccagctgaat aatcgccagg cgaaacgggc
     1141 gcggcagctc ggctttttct ggcgcaacgt cgcgaatttg ctggcgcaga tactcttcat
     1201 taaaacagtg cgcatcaata ccgccaatga aaccaaacgg gttgcgtgaa gcttccagat
     1261 agaccgacgt cgcccccgcc tgaatcagcg cgccgtgatg attcgactta tggttgttac
     1321 ggtcgaagag caccagatcg ccacgcgtta acagcgcatt cgtcaccact ttattcgctg
     1381 ccgatgtgcc gttcagcaca aaataggttt tatcggcatg aaagactttg gctgcgaatt
     1441 tctgcgcatc cttcgccgat ccttcatgaa taagcagatc gcccaatttt acgtcagcgt
     1501 tacacatatc ggcgcgaaag acgttctcac caaagaaatc gtaaaaatgg cgtccggcag
     1561 gatgcttttt aaaaaacgca ccatgttgat gtccagggca agcaaaggtg ctgttgccca
     1621 tctcaacgta ctgcgtcagc gtgtcataaa acggtggcag caaattctct tcatactgac
     1681 aggctgcgga ttccagctcc agccactgct gctcgttgcc gttgattacc gccgtaacgc
     1741 ccgcaggtaa ttcaacagca tgttcggaat acaaaaacac cggtagatga aaaccggtgc
     1801 gcttaagcaa cgcaagaatg ccactgcgac tatccgcagc ggtaatgacg actgccgcga
     1861 catccgtaaa atcagtatct cccaacgcca ccacgcggcg atgagtagaa agtcgggata
     1921 ccagttcact actggcggca atattcattg atttcataag cgcaaaaccc gtttcgggga
     1981 agtaagaata ccggacaaag tgtaaaaccc tgccccatga gatatgggtc aaactggtca
     2041 ccagctccga cgaccgccag acatcagtaa aagcagaaac gctctgattt tactgttgtc
     2101 ctgcagtgaa cgtgcgttaa cttcaccgca tgagcagtaa catagaaagg gaaacgtttc
     2161 gcgcgaaacg gcgataagcg agagaatgta aggagatggc gtgcatcggg caaactccgt
     2221 acagagagga gaaaattcgc gcaatcatgg cacctttcgc taaggcgtgc aagccaggac
     2281 tttgcgacca gacaagccat caacaaggca aaaagttcat aataagaaaa tcaaacaata
     2341 cagctaacag gaactcttgt ggtcatcggc ccttttatca acgctagtgc agtcttactg
     2401 ggtggcgttc tcggcgcact gctcagccaa cgcttaccgg aacgtatccg cgtctccatg
     2461 acatctattt ttggtctggc atcgctgggg attggtattt tactggttgt gaaatgcgcc
     2521 aaccttccag cgatggtttt agctacgcta cttggcgctc taattggcga aatttgtttg
     2581 ctggagaaag gtgtcaatac agcagtcgcc aaagcacaaa atctgtttcg tcactcacgt
     2641 aagaagccag cgcatgaatc ttttattcag aattatgtcg cgattattgt cctgttttgc
     2701 gccagcggca ccgggatctt cggggcgatg aacgaaggga tgaccggcga tccgagtatt
     2761 ttaatcgcca agtcatttct tgatttcttt acggcgatga tcttcgcctg ctcgctgggt
     2821 attgcggtgt cggtaattag tatcccatta ctgatcatcc agctaacgct agcgtgggct
     2881 gccgcgctga tattaccgct gaccacaccg tcgatgatgg cagacttcag cgccgtaggc
     2941 ggtttattgc tgctggcaac cggattacgc atctgtggca ttaaaatgtt cccggtggtc
     3001 aacatgcttc cagcactcct tctggcaatg ccgctttccg ccgcctggac cgcctggttt
     3061 gcctgacaat gcgtgcaata gcggcaaagt gatgatagat tgtgcagtct gcagtaaatt
     3121 gaagaaattt gattgacgag acgaggcgaa tcaggtttaa tgcgccccgt tgcccggata
     3181 gctcagtcgg tagagcaggg gattgaaaat ccccgtgtcc ttggttcgat tccgagtccg
     3241 ggcaccaaat tcatatcaac ggacctccac ggaggtccgt ttttcgtttc agaacgccac
     3301 gatttaagcg ttctgcctac aaatcaattc taccgaactc aaccagattc cccccacatc
     3361 aaccccattg tgtgggtata attgcgggca taccccagtt cgacagaatt tgtaccctct
     3421 ttaacgcatt cagaaggctg aactatggca ctgactgacg caaaaatccg ggctgcaaag
     3481 cccactgaca aggcttataa actcactgac ggggctggca tgttcctgct ggtacatcct
     3541 aatggctccc gttactggcg tctccgttat cgtattctgg gtaaggagaa gactctggca
     3601 cttggtgtgt atccagaagt ttctctctcc gaagctcgta caaaacggga tgaggcccga
     3661 aaactgattt cggagggggt tgacccttgc gaacagaaaa gagctaaaaa agtagtccct
     3721 gatttacagc tctcttttga acatattgcg cgacgctggc atgccagtaa taaacaatgg
     3781 gcacaatcac acagcaataa agtactcaaa agccttgaga ctcacgtttt cccctttatc
     3841 ggcaaccggg atatcacaac actcaatacc ccggatctgc ttatccctgt tcgtgctgca
     3901 gaagctaaac aaatttatga aatcgccagt cgtctgcagc aaagaatatc tgccgtaatg
     3961 cgttatgccg tacagtctgg catcatcaga tataatcctg ctctggatat ggctggcgca
     4021 ttgactacgg taaaacgcca gcatcgcccc gctcttgatc tttctcgcct gcctgaactt
     4081 ttgtcgcgta ttggcagtta caaagggcaa cctgtcaccc agcttgccgt tatgctgaat
     4141 ttactggttt ttattcgttc cagtgaactc agatacgccc gctggtctga aattgatatt
     4201 gacaatgcca tgtggactat tccagccgaa cgcgaacccc tgccaggcgt aaaattctca
     4261 caccggggct ccaagatgcg aacaccacat cttgtgccac tcagcaaaca ggctgtagcc
     4321 atactgacag aactacagac atgggctggt gaaaatggtc tgatctttac gggagcacat
     4381 gacccgcgta aaccaatcag
//
LOCUS       DAJVQG010000216         4326 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJVQG010000216 DAJVQG010000000
VERSION     DAJVQG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.219"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..579)
                     /locus_tag="OQM83_003788"
     CDS             complement(<1..579)
                     /locus_tag="OQM83_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1521919.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPN"
     gene            complement(642..1091)
                     /locus_tag="OQM83_003789"
     CDS             complement(642..1091)
                     /locus_tag="OQM83_003789"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCS1521920.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(1088..4180)
                     /gene="ebgA"
                     /locus_tag="OQM83_003790"
                     /pseudo
     CDS             complement(1088..4180)
                     /gene="ebgA"
                     /locus_tag="OQM83_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
BASE COUNT         1027 a         1090 c         1167 g         1042 t
ORIGIN      
        1 gttcgggatc atcgcgtcaa cggtgatggc gtcagcaggc tgtacgccgc caaccagcgc
       61 cgtacctgcc agtaaaatgt aggagagcgt taacagcagc atcagcgttg aagtgacgga
      121 ggtaattggc cccaacattt tcgccccgtt ggtggaaacc caggtggaga aggcgaacag
      181 caccatactg ataatggtgg tggcaaccgg cgtcataata tattcgtatc cgaggaaggc
      241 atacgaagca taagcaataa cgcgcggcaa cagtgaggtg aaaaagaaca ggtttacgaa
      301 ccagtaggta taggcagtaa taaatgccca acgtccgccc agcgaacttt ttacccatgc
      361 gtagacaccg gcttctgagt ttttatttaa cgaaacaaat tctgcgatga tcagacagaa
      421 gggaataaaa taaaaaatcg tcgcgaggaa aaacatcggt gccgaggcca gtccaagatc
      481 aatattatta ttgataacgt tgttaaagct gtaaacggcg gcaaaagtca gcgagagcaa
      541 gccgaatttg ccgattgtat tacgtttggt atcagacata acacatctcc gaaaatgagt
      601 agggtagcgg tggcggcaaa ctccttttgc cgccgtagtt gttatttgtt atggaaataa
      661 ccatcttcga tggtgacttt gagaaccact tttttgaccg cgttattgca gataaaccga
      721 tacgcttcat ggatatcgca aataacgatt tgcccttcgt ggacctcaac ggtttctccg
      781 cagcctttta aatattcacg gtcagtttca tcacgataat attccactac ctgtaatgtc
      841 tctttcggcg catattcaat tttttgctgc ccttgcaggt agtaatgcac ttcaaaatag
      901 cgtcgatgcc cggtaaatag cgcatcggtc gcggagtctg tctccacgcg gtaagtcaat
      961 gagtcaccga tggagtgggc gacgccaggc tgaatgttgt cgatattttc aatcgcttca
     1021 acgcagcgtt gccacttctt gccagaggcg taaatctggc ggaactgttc taagttatcg
     1081 atgatcctca ttgcttattc tcgctgtgca aattcgtgga aaagaaccct gcgccgaact
     1141 catacgacgc caggctttgc gcggtagctt ctccgccaga aaccggcagc aacgtaaagc
     1201 cgtagctgaa gtcacggaac cagacgcgcc aggagtccag cacctcgctg ccccaggagt
     1261 tggagccgag gccaagcagc tggtgatcga ggttcagggt gatgtcatca ctgcgctgca
     1321 gctcgttaca gtgctgggca gcgtggatgt tttcctaggt atagtgccag gcgctgaagt
     1381 taattgggcg ctgcggaacc accagcagac cgttaccgtg gcggttagtc agtgccgtcc
     1441 agcggacatg ctgacggtta ccgttgttct gcgggaaggg atagttctcg aacatggcat
     1501 cgacggtgct gcgccagata tcgatgatgt tagcctgctg gctgtcggcg tagttttcgc
     1561 ccggtccacg accgtaatac gccacctgat cgtattcgcc gttaattccc atggtgaaac
     1621 cgatgcacgg aatgatgtgc ggatagtcgc cgtaacgctc gccggaaagc gccacgttaa
     1681 cctggccatc ggcagcgatg cgccagatgt aggtgcagcg cattccgaaa tcaaacaccg
     1741 gcggggcaat aaccgtgcgg ctgatgatca acacttcacc ctcgctctgt tctacggcaa
     1801 agtcgcgcag atgttcctgc atgatctgca aatgattcgg ctgccacaac ccttcgtact
     1861 cctgcttgtg gttgtcgatc atcggcttga agaagttgat ctttggctcg cgggtcagca
     1921 gcgactcgcc atttacctgc caggatgtcg gtttgccact catttttgag aaggtgatcg
     1981 cgaagttgta gccgcgaacg gtgcagctca aacgatcgtc ttccagcgtc agcggacgcg
     2041 cattatttgg tgcgaaaggc actggctgcg cggtgttttc cttcagcggg aactgataag
     2101 tggcgataga atgcccggct tcgctgtagc gggtgcggga atctttggtc accgtaatgt
     2161 tgaggaacgc ttcgcgggcg tccagctgcg gcagcgtgat ctgcaagggg gcttcgctgt
     2221 tcggcgcaac gtcgcgcagt ttaatctgct gcgtcgcgag cgtttcacct tcggcgcgca
     2281 cctctgcgtg cagggggtag tcatcaagcg tggtaaacca cagtttattt tcgactttca
     2341 actcgccgcg agtcagatcc cgcgcgtgga tttttaccgg cgcgataacc tgtttgtact
     2401 ctttcaggcc cggtcccggc gtctgatcgg aatagatcaa accatcaaga cagaagttat
     2461 agttgttggg atagtcgccg tagtcgccgc cgaatttata ccagacattg ccgtggtcgt
     2521 cctgtgcctg gatcccgtgg tcgcaccact cccagacata atgaccctga atgcaatcgt
     2581 gcttatagaa gacgttctgg tactccgtca gcccgcccgg tccgttcccc atcgcatgag
     2641 catattcaca gatgatgcgc ggcttcggat gcgggtattc accaaactca ttcatcagcg
     2701 gcacgcgggt gtacatggtg gaaataatat cgaccacttc agcatcgcga tcttcttcgt
     2761 aatgcaccag tcgcgtgtca tccagcgcct tcgctgcgtg gtacatcgcg cggatgttac
     2821 agccatagcc ggattcattg cccagcgacc agatgatgat cgacggatgg tttttctgcg
     2881 cgtgaatatg gcgaacaatg cgctcgacgt agactttttc ccactgcgga tcgtcggtaa
     2941 tacggctgat atcgccgaca ttagcaaagc cgtgcgattc gacgtcggtt tccgccatca
     3001 caaacaggcc gtagatatca cacagttcgt aaaaacgcgg atcgttcggg tagtgagcgg
     3061 tacgcacgga gttgatgttg tgctgcttca tcaactggag atctttctcg acgcgatcca
     3121 ttccaacggc gcggcctttg cgatgatcgt tgtcgtgacg gttgacgccg tgcagcatca
     3181 cataacggtt attgatccag aacagaccgt cgcgcacttt gatatcacgg aagccaacgc
     3241 gttgtggtac cacttccaga acgttgccgt tggcgtcttt cagcgtcatg accagatggt
     3301 aaagataagg ggattctgct gaccattgct gcggctgttc gacagtaaaa gcaaagctgg
     3361 cgctggtcag tttttcaatt gccaaatgat caatggcgct gctgtgcacc acgcgttcgc
     3421 catcaaacag ggtatattcc agcgtcgtga cgacagggga ggcggcgaga ttttccagca
     3481 ccacttcgca ggaaagcgtg gcatcgcaat aggcttcgtc aaagtcggta cgcacggtga
     3541 aatcgttaat atgtgttagt tgttttccga tcagataaac atcgcggaag atccccgctg
     3601 accaccacat atcctggtct tccacgtagg tagagtccgc ccattgcatc acgcgcacac
     3661 acaacaggtt gtcgccggtt ttaaccatcg cgctgatgtc aaactctgcg gtcaggcgac
     3721 tgcccttgct gaaacccaca tactgaccgt taacgtagac ttcaaaatag gtttcgacgc
     3781 cgtcaaattt aatcagcgtc tgtttaccct gccagccgtc gctgagggtg aaaatacgtt
     3841 gataggcacc ggttgggtta tcgctgggaa caaacggcac atcgatgggg aacggaaaac
     3901 cttcgtcggt atattgcagt ttgccgtgac cttccatttg ccacatggcg gggacggtaa
     3961 tatgccccca gtcagccatt aactcagagg tgaaggcttc aggcacttgc agcgggtggt
     4021 caaaaaagtg gaaattccac tgaccgctta agggcagaaa caggctgctg gtttcgcggg
     4081 caaaggtacg cgcttgcgca acagaatcat atgaaaaaaa gtacgcacgt ggcgcaagtc
     4141 ggttttcgtg ggtgagctga atgttttccc agcgattcat agggcctccc ggtagagaga
     4201 aatcggaacc attcatggtg atggtagttt gtgtaattgc tggggagttt agtaaaattt
     4261 ttactaaaaa gtgacaggta aatgcattta ctttaactgg atcacgaaaa taaacggcag
     4321 cactgt
//
LOCUS       DAJVQG010000217         4279 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJVQG010000217 DAJVQG010000000
VERSION     DAJVQG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.220"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(78..1442)
                     /locus_tag="OQM83_003791"
                     /pseudo
     CDS             complement(78..1442)
                     /locus_tag="OQM83_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
     gene            complement(1507..1626)
                     /locus_tag="OQM83_003792"
     CDS             complement(1507..1626)
                     /locus_tag="OQM83_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCS1521921.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1781..1999)
                     /locus_tag="OQM83_003793"
     CDS             complement(1781..1999)
                     /locus_tag="OQM83_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1521922.1"
                     /translation="MKKLLRTLSAVDARVVCKPHRLGRSMWHLVVLLEELCKRGINFR
                     ALAQSIFAQQWGDECCKSKRICDLKVIV"
     gene            complement(2353..3831)
                     /locus_tag="OQM83_003794"
     CDS             complement(2353..3831)
                     /locus_tag="OQM83_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HCS1521923.1"
                     /translation="MLVSKSNEFNTSSFIDSGNCNERKSSESMELLAYSIINLICKEA
                     ASGTYRGALETLQKMMSECIYHEGNAFVIMGSGEQLKRIKYDVDENNLKVFNVYFDNN
                     EELVTDGEPDVVCLSKQVWEDLLIKLKLENKENAVSETDLSSNKNNVDQFIECAKRNE
                     QTLFDNIRKSDFHVDSFKPGSTRSVILETQPNVCMEPHNLYDNQIDKVSPVTKNSQQV
                     KGHYGDKFKEMQMFLNQMSNALQQTPSLSESKEHTIDIQKITDAFVKEFRGVLIDKNG
                     NCSERLFNFYECCYKFLPRAQPQDKIESYNSALQAFSIFRSSTLNNNDVGFNFKLFPE
                     VKLSGENLETVFKYKKGSFVREIARINITLQKEEDGLYNLGGLDFKGCFFSGQNFSNY
                     DIQYVNWRTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGV
                     APFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT         1211 a          932 c          757 g         1379 t
ORIGIN      
        1 actagtctaa aaactagtat taagactatc acttaaataa gtgatactgg ttgtctggag
       61 attcaggggg ccagtctagc aactaaagca ctgcccgtct cctggttaac cccatgtgct
      121 ataccgccgg tatagacaat cagcgagcct ccggcgctaa tattgctgcc agttgccgta
      181 ccatctttct caacaacctg ccggctcccg gtggaaatat ttgtcgtgtc agctttcccg
      241 ccgctcttga tattttgtgt tccgctgttg atattggtac ccgtggcaat accatgatta
      301 ttaatattct gtgtaccacc attgattatg gtattggtcg cgtttctggt aacatccatt
      361 accccgccgt tatctattcg ggtcgcatca gctttagcat tcgttaaaac tgacattgtt
      421 cctttatctt taataatcgt cttatttgcc gaaccatatg cgtttatgtc taaatgacca
      481 ccgttttcca gcaacacatt gtctgccacg tgattgtgga tggagaatgc accttcacta
      541 ttcgtaccgc tcaccgtcgt accgttagtg tttgttttta aaattgcacc atcgtgctgg
      601 gtaacatttg ttgccgtacc accactaaca tcaagcacgc caccggaata aacttcaata
      661 acatccgagg agctggtgta atcaacaatt tgcgtaccac cagaatagat ctgagtattt
      721 tttgccgttg acttattatt cagagactga gttccacctt caatcgtcgt gtccagcgca
      781 tggctttcat atactctttg ctcaccgcca tttttaatgg tggttgttcc tactgtgctc
      841 tgttcaacat actgtcgtcc accatttatg attgtgttcg ttgccatact tccttgcgtg
      901 atgtcctgat aaccggactt atttatcgtt gtaccatcaa catgcccctg agttgttact
      961 gtctgccctc caccatcaat gatggttcca ttcgcagtcc ccccacttat gtgtgaatta
     1021 ccacccttaa ttgtcgttcc attgctgata ccgcctgtat aaacgtcctg attgccactg
     1081 tcgattatcg taccggtgga aatgcccccg tcatgaattg tctgtcttcc tccgttaatg
     1141 gttgtattat tagactgccc catataattg ttattccttc caacatcttg atatccacca
     1201 gattcaataa gacttccatt agatactccg ccataaacac tctgctggcc atggttgata
     1261 atatgagtgt tatttgttgt acctcgttca tctactcgtt ggttgccatc tacagtctca
     1321 tcgtttacca caccaataac attagtagta aaggccgcca tcacgggcgg ggcatatatc
     1381 aaggcagata tcaataagga aagtactgag cggcgacaat aatggggact ggtcctgttc
     1441 ataaatttca tcctctgaaa agtgaatact gagtagcgtt taagcgactt tagctctgct
     1501 gcaacatcag cccgcaggca ccagaccggg gggttcatcc tgaagagaca gcgcaagtgt
     1561 attgtgttca ccgctcatca aagacatcat gatgaaatga tgatgttcca cataagaaag
     1621 aggcattttt taaacacagt gcgctgaagt gtgattggat aaaaaagtca atctattcag
     1681 gaaatacggg cgtattcttt tctttcgaca atcaggccgt cggcaaaata aaatgattta
     1741 cataatcgtt tctgatgaat atcttctgct cacataaaaa tcacacaata actttgagat
     1801 cgcagattct tttactttta cagcattcgt ccccccattg ttgggcaaat atagattggg
     1861 ccagagcacg aaagttaata ccacgtttgc acagctcctc caacagcacg acaagatgcc
     1921 acatactgcg ccccagtcgg tgcggtttac agactacccg tgcgtccacc gccgataatg
     1981 tcctgagcag ttttttcagt ccggacctcc tgccacggtt ttacgttgaa gaggtaaccc
     2041 tgagcacgca gttcttcagt caggcgtggt gcaccgtaac gctgttattg ctgggtaaga
     2101 tcaaaaaact ttcaggcagc taaggaaagt tgaaccagac attagatgaa atatttcaac
     2161 caattacagc accaatttaa actcagtaaa tcacagcaca gatcaagggg ttacgtgaaa
     2221 gcgtagcccc tttttctttg gtagtggcag caaaatggtt gtagtgtaaa aaataatccc
     2281 gtttaatcaa tcaataatac atattgtttc aatctacgtt attatctctt tgtaaaaata
     2341 gccatttatt aatcattgaa aactgctttt agaacttgat acaacgggac tagtcacaac
     2401 aggactattc tcaacgggat catcctcaga ggaactatca tcaaagtcat catccataaa
     2461 taaaatatca tcgaatggtg ccacgcccgt gatgagtttt attttattat tacgatcagt
     2521 caagactcca cttaaaccgt tttcgctcac aggttttaat gatttttcat tactcttgtt
     2581 gtaagcaggc gcattaaaaa tacacggagt atcaagatca aacaatgacg ttctccagtt
     2641 cacatattga atatcatagt tactgaagtt ctgtccagaa aagaagcatc ccttaaaatc
     2701 caatccacct aaattatata aaccatcctc ttctttttgg agagtaatgt taattctggc
     2761 tatctcccgg acaaaacttc cttttttgta tttgaatacc gtttcaagat tttcaccaga
     2821 cagcttgact tctgggaata atttaaaatt aaaccctaca tcattattat tcaaagtaga
     2881 tgaacgaaag atggaaaaag cttgtagtgc tgaattatag ctttcgattt tatcttgagg
     2941 ctgcgctctt ggtaaaaact tatagcagca ctcataaaaa ttgaataaac gttctgaaca
     3001 attcccattt ttatcaatta ataccccccg gaattctttc acaaaggcat ccgttatttt
     3061 ttgaatatct atggtgtgtt cctttgactc tgacaaagat ggagtctgtt gaagtgcatt
     3121 gctcatctgg ttaaggaaca tctgcatttc tttaaattta tctccatagt ggcctttaac
     3181 ttgctgagag tttttagtta caggtgaaac cttatctatt tggttgtcat ataaattatg
     3241 tggttccata cagacatttg gttgtgtttc taaaataaca ctacgtgtac tacctggctt
     3301 aaatgaatca acatgaaaat cactttttct tatattatcg aatagggtct gttcatttct
     3361 cttagcgcat tcaataaact gatcaacatt atttttattc gatgacaggt cagtttcaga
     3421 aaccgcattt tctttgttct ccagctttag tttaatgaga agatcttccc agacctgctt
     3481 gcttaaacat actacgtcag gctcaccatc agtaactaac tcttcattat tatcaaagta
     3541 tacgttgaat acttttaagt tattttcatc aacatcatat ttaatacgtt ttaattgctc
     3601 tccagatccc ataatgacaa aggcgttgcc ttcatgatat atacattcag acatcatttt
     3661 ttgtaaagtt tcaagagcac cgcgatacgt ccctgaggca gcttccttac aaattaaatt
     3721 tataatacta taagctagta gctccatgga ttcagaagat tttctttcat tacaatttcc
     3781 actatctata aatgagctag tgttaaattc gttgctttta ctaactaaca ttgtctattc
     3841 ctcagttaat gtctacatgg ctatttttaa ttttattatt gtttgtcact ataaaaaatc
     3901 gctcatttga gacaattgct ggcattaaca gcttcatatg ctatacatgg tacttttaat
     3961 taaattagca caagaatgtt aaatttaata aacaaaaagt tatttcgctg tatgataaaa
     4021 accacccgtt ataatttatt agtgaaattc gtttttcgag tgttagaaat ttatatctaa
     4081 atagcgttga ttaatgagca aagccagact cctgtaacgc tcacaagact catctacctg
     4141 cgacgggtat tctggtcagt agtagatgtt taaggcgtgg cagagacatt tcatccttac
     4201 tctacggcat tggcccgcat acaaatcgtg gttgtgacac tcacttatca tcagtgagcg
     4261 aacagagaat agttcagtg
//
LOCUS       DAJVQG010000218         4267 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJVQG010000218 DAJVQG010000000
VERSION     DAJVQG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.221"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            288..896
                     /locus_tag="OQM83_003795"
     CDS             288..896
                     /locus_tag="OQM83_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCS1521924.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            994..2385
                     /gene="selA"
                     /locus_tag="OQM83_003796"
     CDS             994..2385
                     /gene="selA"
                     /locus_tag="OQM83_003796"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCS1521925.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2382..4226
                     /gene="selB"
                     /locus_tag="OQM83_003797"
     CDS             2382..4226
                     /gene="selB"
                     /locus_tag="OQM83_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCS1521926.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          967 a         1078 c         1241 g          981 t
ORIGIN      
        1 acaatgctac cgccatactg cgtcatgtcg ccctgacgcg ctgccggttt tccgctcatc
       61 tgctcatcct tgttttatcg ggttatttgt ctgcttccag gcaaaaccct acagatttat
      121 aagaaagcct taagtgagat atccatcaga tagaaacaat taggaaaata tttatatcac
      181 catgataaat atgtgattta tttggaagta acaaaccatc aacattttct gccctttttc
      241 ccatcaccag gccatactga tatttttcct cgcacaaagg actcggcatg aaactcgtcg
      301 gtagctacac cagcccgttt gtacgcaaac tttctattct gttgttagaa aagggcataa
      361 ctttcgaatt tattaatgaa ctgccctata acgcggacaa cggcgtggcg caatttaacc
      421 cgttaggaaa agtgccggtg ctggtgaccg aagagggcga atgctggttt gattcgccga
      481 tcatcgctga atatattgag ttaatgaatg tcgctccggc gatgttgccg cgcgatccgc
      541 tggagtcgtt gcgggtgcgc aaaattgagg cgctggcgga tggcattatg gatgccgggc
      601 tggtatcggt gcgtgaacag gcgcgtccgg cggcgcagca gtctgaagat gaattgttac
      661 gccagcggga gaaaatcaac cgcagtctgg atgtgctgga aggatatctg gtcgatggca
      721 cactcaaaac cgatacggtc aatctggcga ctatcgccat tgcctgtgct gtcggatatc
      781 tcaatttccg ccgcgttgcg ccaggttggt gtgtcgaccg cccacactta gtcaaactgg
      841 tcgaaaacct gtttagccgc gaaagttttg ctcgcaccga accgccaaag gcttgatgcg
      901 cgatatgtcc tcctgaccca tctcacgtta caatccgtgg ttatgttaaa cgcccttctc
      961 cgtgtgagag ggccttgatc agccaggttt cctatgacaa ccgaaacgcg ttccctctat
     1021 agtcaacttc cggctattga tcgcttattg cgcgatagct ccttcctttc tttgcgtgat
     1081 acttatggtc acacccgcgt ggtggaattg ttgcgtcaga tgctcgacga agcgcgagaa
     1141 gtgattcgtg acagccagac gcttcctgcg tggtgtgaaa actgggcgca agaagtcgat
     1201 gcccggttga cgaaagaggc gcagagcgcg ctgcgtccgg tgatcaacct gacgggaacc
     1261 gtgctgcata ccaaccttgg acgagcttta caggcggaag ccgcggtgga agccgttgcg
     1321 caggctatgc gttcgccagt gaccctcgag tacgatctgg acgacgccgg acgcggacat
     1381 cgcgatcggg cgctggcgca actgctgtgc cgtattacgg gggcggaaga tgcctgtatc
     1441 gtcaataaca atgcggcggc ggtgttattg atgttggcgg ccactgccag cgggaaagag
     1501 gtggtagtat ctcgcggcga attggtggag attggcggcg cgtttcgtat tcccgatgtt
     1561 atgcgtcagg caggctgcac cctacacgaa gtagggacca ccaaccgcac gcacgcgaat
     1621 gattatcgtc aggcggtgaa tgaaaatacc gcactgttga tgaaagtaca taccagtaac
     1681 tacagcattc aggggttcac caaagcgata gatgaagcgg aactggtggc gctcggcaaa
     1741 gagctggatg ttcccgtagt gactgattta ggcagtggct cgctggtcga tcttagccag
     1801 tacggtttgc cgaaagagcc aatgccgcag gagttgattg cggcgggcgt cagtctggtg
     1861 agcttctccg gcgacaagtt gttaggcggg ccgcaggcag ggattatcgt tggtaaaaaa
     1921 gagatgatcg cccgcctgca aagccacccg ctgaagcgtg cattacgcgc ggataaaatg
     1981 accctcgcgg cgctggaagc cacgttgcgt ctttatttac accctgaagc tctgagtgaa
     2041 aaattaccga ccctgcgcct gcttacccgc agcgcagagg tcattcaaat ccaggcacaa
     2101 cgtttacagg ctccccttgc cgcacattac ggcgcggagt ttgcggtaca ggttatgcca
     2161 tgtctttcgc agattggcag tggttcgctg ccggttgatc gcctgccgag cgcggcatta
     2221 acgtttacac cccatgatgg acgcggtagc caccttgagt cattagccgc ccgctggcgt
     2281 gaattgccag tgccgatgat tggtcgtatt tatgacggac gattgtggct ggatttacgc
     2341 tgccttgaag atgagcaacg gtttttggag atgttgttga aatgattatt gcgactgccg
     2401 gacacgttga ccacggcaaa acaaccttat tgcaggtgat tactggcgta aatgctgacc
     2461 gtctgccgga agaaaaaaag cgcggcatga ccatcgatct cggctatgcc tactggccgc
     2521 agccggatgg tcgcgtgcct agttttatcg acgtacccgg tcatgaaaag tttctttcca
     2581 acatgctggc gggcgttggt ggtatcgatc acgcgctgtt ggtggtggcg tgcgatgacg
     2641 gcgtgatggc gcagacccgt gagcatctgg cgattttgca gctgaccggt aacccgatgc
     2701 tgacagtggc gctgaccaaa gccgatcgcg tggacgaagc gcgtgttgat gaggttgaac
     2761 gccaggtaaa ggaggtgctg cgggaatacg gttttgctga ggcaaaactg tttatcaccg
     2821 cagcaaccga aggtcgggga attgatgccc tgcgcgagca tctgcttcag ttaccaggac
     2881 gtgaacacgc cagccaacat agtgtccgcc tcgcgatcga ccgcgcattt accgtaaaag
     2941 gtgccggtct ggtcgtcacc ggtacggcgt taagcgggga agtgaaggta ggcgattcac
     3001 tctggctgac tggtgtaaat aaaccgatgc gtgtacgtgc gctgcatgca caaaaccagc
     3061 caacagaaac cgcccatgcc gggcagcgta tcgcgcttaa catcgcgggt gatgcggaaa
     3121 aagagcagat taaccgtggc gactggctgc ttgccgatgc gccgccagag ccgttcacac
     3181 gggtgattgt cgagcttcaa acccatacgc cgctgaccca gtggcagccg ctgcatattc
     3241 accacgccgc cagccacgtt acgggacgcg tttcgctgct ggaagataac cttgccgagc
     3301 tggtgttcga cacgccgctg tggctggcgg ataacgatcg cttagtgctg cgcgatatct
     3361 cggcccgcaa cacgctggcg ggggcgcgtg tggtgatgct taacccgccg cgtcgcggta
     3421 aacgtaagcc ggaatatctg caatggctgg cgtctcttgc acgggcgcag agcgatgccg
     3481 atgcgttatc tgttcatctg gaacgcggcg cggttaacct tgcggatttc gcctgggcgc
     3541 gccagctcaa cggcgaaggg atgcgcgaat tgctgcaaca gcctggctat attcaggctg
     3601 gttatagctt gttgaatgcg ccggttgccg cccgctggca gcggaaaatt ctcgacacat
     3661 tagcgactta tcatgagcaa catcgcgatg aacctggtcc tgggcgcgaa cgtctgcgac
     3721 gtatggcgtt gccaatggaa gatgaagcgc tggtactgtt gctgattgaa aagatgcgcg
     3781 aaagcggcga catcctcagt catcacggtt ggctgcatct gccggatcac aaagcgggct
     3841 tcagcgaaga gcagcaggcc atctggcaaa aagcagagcc gctgtttggt gacgagccgt
     3901 ggtgggtgcg tgatctggca aaagagacgg gtaccgacga gcaggcaatg cgcctgacgc
     3961 tacgccaggc ggcgcagcaa ggaataatta ccgcgatcgt caaagatcgt tattaccgta
     4021 acgatcggat tgtcgaattt gccaatatga tccgcgatct cgatcaggag tgtggttcaa
     4081 cctgcgcggc ggatttccgc gatcgcttag gcgtaggccg aaagctggca attcagattc
     4141 tggaatattt tgaccgcatt ggctttacgc gtcgtcgtgg aaatgatcat ttattgcgcg
     4201 acgcattatt atttccggaa aaataaggaa atgattaatt aatttttaaa ataaattaat
     4261 acaaatt
//
LOCUS       DAJVQG010000219         4240 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJVQG010000219 DAJVQG010000000
VERSION     DAJVQG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.222"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..613
                     /gene="phnP"
                     /locus_tag="OQM83_003798"
     CDS             <1..613
                     /gene="phnP"
                     /locus_tag="OQM83_003798"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCS1521927.1"
                     /translation="ITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVG
                     DPIPIYGPPDEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYL
                     LETAHSRVAWLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLAL
                     NQVIRSPRVILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(760..1089)
                     /gene="yjdP"
                     /locus_tag="OQM83_003799"
     CDS             complement(760..1089)
                     /gene="yjdP"
                     /locus_tag="OQM83_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HCS1521928.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(1158..1475)
                     /locus_tag="OQM83_003800"
     CDS             complement(1158..1475)
                     /locus_tag="OQM83_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HCS1521929.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            1728..3713
                     /gene="yjcS"
                     /locus_tag="OQM83_003801"
     CDS             1728..3713
                     /gene="yjcS"
                     /locus_tag="OQM83_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HCS1521930.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            3922..4197
                     /locus_tag="OQM83_003802"
     CDS             3922..4197
                     /locus_tag="OQM83_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521931.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         1071 a         1116 c         1019 g         1034 t
ORIGIN      
        1 gatcaccctg atcgacgccg ggctgcacga tctcaccgat cgctggtctc ccggatcgtt
       61 ccagcagttt ttgctgacgc attatcatat ggatcacgtc caggggctgt ttccgctgcg
      121 ctggggcgtg ggcgatccga tcccgatcta cggcccgccg gatgaacagg gctgcgacga
      181 tctgtttaaa catcccgggc tgcttgattt cagccacacg gtggaaccgt ttgtggtgtt
      241 tgatttgcag gggttgcagg tcacgccgct gccgcttaac cactcaaaac tcactttcgg
      301 ctatttactg gaaacggcgc acagccgggt ggcgtggctg tctgacaccg ctggcttgcc
      361 ggaaaaaacg ctgaaatttt tacgcaataa tcagccgcag gtaatggtga tggattgcag
      421 tcacccgccg cgcgcggatg caccgcgtaa tcactgtgat ttaaataccg tgcttgcgct
      481 gaatcaggtt atccgctcgc cacgggtgat tctgacccat atcagccacc agtttgatgc
      541 gtggctgatg gaaaacgcac taccgtcagg gtttgaggtg gggtttgatg ggatggagat
      601 tggggtggcg tgatgagagg gaatgtgcgc gctggccccc tcaccctaac cctctcccca
      661 gaggggcgag gggaccgatt gtgctcaata ttgaatattg cgctcgtttt ctccctctcc
      721 ccattggggt gaggggcgat gcctgctcca tacccaacct catcgcccat actcatcttc
      781 cattctccgc tcttcatcct ccagttgccg acgctcctga tcaagctggc gctggcgatc
      841 gtccagctgc ctgcggcgat cttcaaactg gcggcggcgg tcgtcatatt gtctgcgccg
      901 atcgtcgctc acttcacgct gccagccgtc gtcgcgcgaa tcttcatagt ctcgcccacg
      961 gtcagggtta taagcgtcat taatcgcctg ctgaatatta ccaatagtat cgtcgataat
     1021 atcagcctgg gccggaacgg tggacagcgt gagcagggtg aataaaagaa atagcggaaa
     1081 gcgtttcatt agccaacctc aaaaagaaac tctatccaca ttagtcatta ctcatccatg
     1141 caagtagtgg atgaatctca atttctccgc tgctctattg ccgtaatcgc ctccacgcgt
     1201 tgttgatgac gaccgccttc gtactgtgcg cccagccacg catccacaat catttttgcc
     1261 agttcgaggc caaccactcg tgaaccaaaa gccagtgcaa cttcactggc ataatgcgga
     1321 taatcagtgc gctctgacga cctggtgtct ttatcaatca cctcactcat tgaccatgaa
     1381 taaaaggctg gcacagcaac gcagaatcct gcggtgccag cgacatgacc gcccattgct
     1441 ccggtacgtt ttttaaaaag aaacaccggt tgcattgata aattgtgctc agtatttgcc
     1501 gaagtacaat atatataata aatatataaa acacctcatg ttatttacag agataataaa
     1561 ttcgcgtaat tttccctctt cagcacaaat gcctaataaa atggatcgtt cagctcacac
     1621 ttccacttat taaagtcgca aattcccaat tatgataacc ccagctttaa atgacgtatt
     1681 ttgcatggat tacatattta cgacataaat gataaatgga gatttacatg aataactctc
     1741 ggttattccg tttgagcaga attgttattg cgttaactgc cgccagcggc atgatggtaa
     1801 ataccgctaa cgcgaaagag gaagcgaaag ccgccactca atatacccaa caggttaatc
     1861 agaattacgc caaatcatta ccgtttagcg atcgtcagga ttttgacgac gctcagcgtg
     1921 gatttatcgc tccgctgctg gatgaaggta ttctgcgcga tgctaatggc aaaccatatt
     1981 atcgcgggga agattataaa tttgatatca atgcgcccgc gcccgaaacc gttaacccta
     2041 gcctgtggcg tcagtcgcag ttaaacggta tttccggcct gtttaaagtc accgacagaa
     2101 tgtaccaggt tcgcggtcag gatatctcca acatcacctt tatcgaaggt gataccggga
     2161 ttattgtcat cgacccgctg gtgacgccac cgagcgcaaa agccgccctt gacctttact
     2221 tccaaaatcg cccgcaaaaa ccgattgttg cggttattta tacccacagc cataccgacc
     2281 actatggcgg cgtgaaaggc attatctctg aagctgatgt gaaatccggt aaagtgcagg
     2341 ttatcgcccc tgctggcttt atggacgaag ccatcagcga aaacgtactg gcgggtaata
     2401 ttatgagccg ccgtgcactt tactcctacg gcctgctgct ggcgcataac cctcagggta
     2461 acatcggcaa tggtcttggc gtaacgctgg catcgggcta cccgagcatc atcgcaccga
     2521 acaaaaccat caccaaaacc ggtgagaaga tgattatcga cggcctggag tttgacttcc
     2581 tgatgacccc aggtagcgaa gcaccagccg aaatgcactt ctatattccg gccctgaaag
     2641 cgctgtgtac cgccgagaac gccacgcata ccctgcacaa cttctacact ctgcgcggcg
     2701 cgaaaacccg cgacaccagc aagtggaccg agtatctgaa cgaaacgctg gatatgtggg
     2761 gtaacgacgc ggaagtcctg tttatgccgc acacctggcc ggtctggggc aataagcata
     2821 tcaatgatta tattggtaaa tatcgcgata ctatcaagta cattcacgac cagaccctgc
     2881 acctggcgaa ccagggctac accatgaatg aaatcggcga catgattaaa ctgccgcctg
     2941 cacttgccaa taactgggcc agccgtggct attacggttc tgtcagccac aacgcccgcg
     3001 cggtgtataa cttctatctt ggctattacg acggtaaccc ggctaacctg catccgtatg
     3061 gtcaggtgga gatgggtaaa cgttacgtgc aggcgctggg cggttctgcc cgtgtcatca
     3121 acctggcgca agaagcgaac aagcaaggtg attaccgctg gtcggcagaa ctgctgaaac
     3181 aggtgattgc cgccaacccg ggtgaccagg tcgcgaagaa tctgcaagcg aataactttg
     3241 aacagctggg ctatcaggcc gagtccgcca cctggcgcgg tttctacctg accggcgcga
     3301 aagagctgcg cgaaggggtg cataagttca gccacggcac catcggttcc ccggacacca
     3361 ttcgcgggat gtcggtcgaa atgctgttcg actttatgtc cgttcgcctc gatagcgcga
     3421 aagccgcggg taaaaatatc agcctgaact tcaatatgag caatggcgat aacctcaacc
     3481 tgacgctgaa cgatagcgtg cttaactacc gtaaaacact gcaatcccaa gctgacgcct
     3541 ctttctacat cagccgtgaa gatctgcacg ccgtgctgac cgggcaagcc aaaatggcgg
     3601 atctggtaaa agcgaagaaa gccaaaatta ttggcaatgg cgcgaaactg gaagaaatta
     3661 tcgcctgtct ggataatttc gatttgtggg tgaatatcgt aaccccaaat taatgtcact
     3721 cgcttttggg ggcgctgaag cccccaaaag catttatttt aaaaatatat acagccaatt
     3781 aataaataaa atatataagt tatgaatgcg gtgactgaat aaagctattt ttattcatcg
     3841 agaaaatagc catactgacg ccttcaatgg atattgatat tcactacaga tattgttcgc
     3901 agcagacagg atgtatttaa tatgccaaca gttctctctc gcatggcgat gcaactcaaa
     3961 aaaacagcct ggataattcc cgtcttcatg gtttcgggat gctcattatc tccggcaatc
     4021 ccggtgatcg gtgcttatta tcccagctgg tttttctgcg ccattgccag ccttattttg
     4081 atgctcatca cgaggcgagt tattcagcgg gcaaatatca atctggcatt tgtcggaatt
     4141 atttataccg ccctttttgc tctctacgcc atgctgttct ggctggcatt tttctaatct
     4201 atttgagacg cgctcatgga aagtacgccg aaaaaagctc
//
LOCUS       DAJVQG010000220         4233 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJVQG010000220 DAJVQG010000000
VERSION     DAJVQG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.223"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..1109)
                     /gene="gntP"
                     /locus_tag="OQM83_003803"
     CDS             complement(<1..1109)
                     /gene="gntP"
                     /locus_tag="OQM83_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCS1521932.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISA"
     gene            1449..2633
                     /gene="uxuA"
                     /locus_tag="OQM83_003804"
     CDS             1449..2633
                     /gene="uxuA"
                     /locus_tag="OQM83_003804"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCS1521933.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLVQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            2714..4174
                     /locus_tag="OQM83_003805"
     CDS             2714..4174
                     /locus_tag="OQM83_003805"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCS1521934.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
BASE COUNT         1024 a         1157 c         1148 g          904 t
ORIGIN      
        1 gcactgataa tcccggcggc ggtcatcgct gaaaccacac cctgacccgt cgccagacga
       61 atcagtacgg tgatcagcca tgccatgatg tagggcgaga tattgccgtg ggacattaac
      121 atgccgatgg tgtcaccaat gccggtgtcg atgatggtct gcttcagcac gccacccgca
      181 ccgatgatca gaatcaccat tgcaatgctc ttcaccgcgc tttcaaaagc gttcatcacc
      241 cactgcatat cgtgcccgcg cgcggtgccg aagagtacga atgcaaccac catcgcaata
      301 aacattgcaa tcggcgagga accgataaag ttaaccactt cccaggcagg ggtatctttt
      361 accagccaga tattggcgat ggtggtagag atcataatga tcgccgggat cagcggcacc
      421 agaatcgaaa cgccgaaaga gggcagatta ttcatatcta ccggttgatc tgctttcagg
      481 aatgatggcg ttgggcgctc aagattgccg aggaacttcg gcaggatcag acctgcgcag
      541 atgacacttg ggatcgtcac cagtacgcca tagatataaa ccatccccat atccgcgcca
      601 taagcattca ccagcgccac cggacccggc tgcggtggga acagtgaatg tgcggtagtg
      661 gcagctgcta ctgccgggat cgccagtttc agaaacggga ttttagcttc ggcggcaatg
      721 acaataacca gcggcgctaa catgataaag gccacttcat agaacatcgc cagaccgaaa
      781 atcaggccga tgataattac cgacagctgt acatagcgca gaccgagacg cgccagcagc
      841 gtatgcgcta tctggtgcgc cgcgccggag tcgaccatca atttaccgat gaccgcaccg
      901 aacaccacga tgatagccag ttcccccagc gtgttgccga agcccgcttt cattgtgtgc
      961 agcagcgaca tcaaatccat gcccgccagc atcccgacgg acagcgccgc cactagcaaa
     1021 gccaccattg aattgatttt gaacttcaaa ttcagtacca gcatcaggcc aatgccgaat
     1081 accacccaga gaatgttaag cacatgcata acgttttacc ttacctggtt gaaccgttgt
     1141 tattttgggc gacatgttat gtaaattggt caaccattgt tgcgatgaat gtcacatcct
     1201 ctgatcaata accatcgatt accctttgct gcaatttgca gcaacaaccg tgagagtgaa
     1261 attcttgcga tgtggttaac caatttcaga tttcgggttg acatgtctta ccaaaaggta
     1321 gaacttatac gccatctcat ccgatgcaac gccacggctg cggtctggtt gttcatccgg
     1381 atacctaaac aactccgggg ctccacgtct ctttgctgtg gaacccacta tgtgaaagag
     1441 gaaaaatcat ggaacagacc tggcgctggt acggcccaaa cgatccggtt tctctagctg
     1501 atgtccgtca ggcgggcgca actggcgtgg ttaccgcgct gcaccatatc ccgaacggcg
     1561 aagtatggtc cgtagaagag atcctcaaac gcaaggcgat cgttgaagac gcaggcctgg
     1621 tatggtctgt cgtagaaagc gtgccaattc acgaagatat caaaacccac actggcaact
     1681 atgaacagtg gatcgctaac tatcagcaga ctctgcgcaa cctggtgcag tgcggcattc
     1741 gcaccgtgtg ctacaacttc atgccggtgc tcgactggac ccgtactgac ctcgaatacg
     1801 tgctgccaga cggctccaaa gctctgcgct tcgaccagat cgaattcgct gcattcgaaa
     1861 tgcatatcct gaagcgtcca ggcgcggaag cggattacac cgaagaagaa attgctcagg
     1921 ccgctgaacg cttcgccact atgagcgacg aagacaaagc gcgtctgacc cgtaacatca
     1981 ttgccggtct gccgggcgcg gaagaagggt ataccctcga ccagttccgt aagcacctgg
     2041 agctgtataa agatatcgac aaagccaaac tgcgcgaaaa ctttgccgtc ttcctgaaag
     2101 cgattattcc ggttgccgaa gaagttggcg tccgtatggc tgttcacccg gacgatccgc
     2161 cgcgcccgat cctcggcctg ccgcgcattg tttccaccat tgaagatatg cagtggatgg
     2221 ttgataccgt aaacagcatg gcgaacggtt tcaccatgtg cacaggttcc tacggcgtgc
     2281 gtgctgacaa cgatctggtt gatatgatca agcagttcgg tccgcgtatt tacttcaccc
     2341 atctgcgctc caccatgcgt gaagataacc cgaaaacctt ccacgaagcg gcgcacctga
     2401 acggtgacgt tgatatgtac gaagtggtga aagcgattgt tgaagaagaa caccgtcgta
     2461 aagcggaagg caaagaagac ctgatcccga tgcgtccgga ccacggtcat cagatgctgg
     2521 acgacctgaa gaagaaaacc aacccaggtt actccgcaat tggtcgtctg aaaggcctgg
     2581 ctgaagttcg cggtgtcgaa ctggcgatcc agcgcgcttt ctttagccgt tgatacccac
     2641 cggcatggct gcgcgccgtg ccggttcctt cttccttgcc gtcactctct gaagacggat
     2701 tctggagttt acgatgacta ctattgttga cagcaatctg ccggttgccc gcccgtcatg
     2761 ggatcattct cgtctggaat cacgcattgt gcatctcggt tgcggggcgt ttcaccgcgc
     2821 gcaccaggcg ctgtacaccc atcatctgct ggaaagtacc gacagcgact ggggtatctg
     2881 cgaagttaac ctgatgccag gcaacgaccg tgtgctgatt gaaaacctga aaaaacagca
     2941 actgctgtac accgtggcgg aaaaaggcgc agaaagcacc gagctgaaaa ttatcggttc
     3001 gatgaaagaa gccctgcatc cggaaatcga cggttgtgaa ggtattctca acgcgatggc
     3061 gcgtccgcag acggcgattg tctccctgac ggtcacagag aaaggttatt gcgccgatgc
     3121 ggcaagcggt cagctggatc tcaataaccc gctgatcaag cacgatctgg aaaacccgac
     3181 tgcgccgaag tccgcgattg gttacatcgt cgaagccttg cgtctgcgtc gtgaaaaagg
     3241 gctgaaagcg tttacggtga tgtcctgcga caacgtgcgt gaaaacggtc atgtggcgaa
     3301 ggtcgcggtt ctggggctgg ctcaggcacg tgatccgcag ctggcggcat ggattgaaga
     3361 gaacgtcacc ttcccgtgca ccatggttga ccgcatcgtt ccggcggcga cgccagaaac
     3421 cttacaggaa attgctgacc agctgggtgt ttacgacccg tgcgccattg cctgcgaacc
     3481 gttccgtcag tgggtgattg aagataactt cgttaatggt cgtccggact gggataaagt
     3541 gggcgcacag ttcgttgcag acgttgtgcc gttcgaaatg atgaaactgc gtatgctgaa
     3601 cggcagccac tctttcctgg cgtacctcgg ttacctcggc ggctatgaaa ccattgccga
     3661 caccatgact aatccggatt atcgcaaagc ggcctttgcc ctgatgatgc aggaacaagc
     3721 gccaacgctg tcgatgccgg aaggtacaga cctgaacgcc tatgcgacgc tgctgatcga
     3781 gcgtttcagc aacccgtctc tgcgtcaccg tacctggcag attgcaatgg acggcagcca
     3841 gaagttaccg cagcgtcttc tggatccggt gcgtctgcac ctgcaaaacg gcggcagctg
     3901 gcgtcacctg gcactgggcg tggctggctg gatgcgctac acccagggcg tggatgaaca
     3961 gggtaatgcc attgacgtgg ttgacccgat gctggccgag ttccagaaga ttaacgcgca
     4021 gtatcagggc gcagaccgcg tgaaagcgct gctgggcctg agcggcattt ttgccgatga
     4081 tctgccgcag aatgccgact ttgttggcgc ggtgacggcg gcatatcagc agctgtgcga
     4141 acgcggtgcg cgcgagtgtg tggctgcgct gtaactaact gattacccta cagacttact
     4201 ggtcaatcaa actgatattt ggttgaccag ttt
//
LOCUS       DAJVQG010000221         4192 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJVQG010000221 DAJVQG010000000
VERSION     DAJVQG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.224"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OQM83_003806"
     CDS             complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OQM83_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1521935.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="OQM83_003807"
     tRNA            complement(330..405)
                     /locus_tag="OQM83_003807"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="OQM83_003808"
     tRNA            complement(412..486)
                     /locus_tag="OQM83_003808"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="OQM83_003809"
     tRNA            complement(603..687)
                     /locus_tag="OQM83_003809"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="OQM83_003810"
     tRNA            complement(696..771)
                     /locus_tag="OQM83_003810"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="OQM83_003811"
                     /gene_synonym="panK"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="OQM83_003811"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCS1521936.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="OQM83_003812"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="OQM83_003812"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCS1521937.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="OQM83_003813"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="OQM83_003813"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCS1521938.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1080 a         1040 c          936 g         1136 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatct tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 acttcccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aa
//
LOCUS       DAJVQG010000222         4148 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJVQG010000222 DAJVQG010000000
VERSION     DAJVQG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.225"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..1358
                     /gene="dcuC"
                     /locus_tag="OQM83_003814"
     CDS             <1..1358
                     /gene="dcuC"
                     /locus_tag="OQM83_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCS1521939.1"
                     /translation="VVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSSQ
                     ASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQY
                     INSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASPA
                     AIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEHI
                     SHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILEF
                     LRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSFG
                     SASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQASN
                     LGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVTG
                     K"
     gene            complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="OQM83_003815"
                     /gene_synonym="citB"
     CDS             complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="OQM83_003815"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HCS1521940.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="OQM83_003816"
     CDS             complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="OQM83_003816"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HCS1521941.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHHNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT         1020 a         1057 c          957 g         1114 t
ORIGIN      
        1 gggttgtggt tattgtgggt gtagctcgct acatcattaa agggtattcc gccactggtg
       61 tgttatttgt cggtggcctg ttattgctga ttatcagtgc cattatgggg cacaaagtgt
      121 taccgtccag ccaggcttca acaggctaca gcgccacgga tatcgttgaa tacgttaaaa
      181 tattactaat gagccgcggc ggcgacctcg gcatgatgat tatgatgctg tgtggatttg
      241 ccgcttacat gacccatatc ggcgcgaatg atatggtggt caagctggcg tcaaaaccat
      301 tgcagtatat taactcccct tacctgctga tgattgccgc ctattttgtc gcctgtctga
      361 tgtctctggc cgtctcttcc gcaaccggtc tgggtgtttt gctgatggca accctatttc
      421 cggtgatggt aaacgttggt atcagtcgtg gcgcagctgc tgccatttgt gcctccccgg
      481 cggcgattat tctcgcaccg acttcagggg atgtggtgct ggcggcgcaa gcttccgaag
      541 tgtcgctgat tgacttcgcc ttcaaaacga cgctgcctat ctcaattgct gcaattatcg
      601 gcatggcgat cgcccacttc ttctggcaac gttatctgga taaaaaagag cacatctctc
      661 atgaaatgtt agatgtcagt gaaatcacca ccactgctcc tgcgttttat gccattttgc
      721 cgttcacgcc gatcatcggt gtactgattt ttgacggtaa atggggtccg caattacaca
      781 tcatcactat tctggtgatt tgtatgctga ttgcctccat tctggagttc ctccgcagct
      841 ttaataccca gaaagttttc tctggtctgg aagtggctta tcgcgggatg gcagatgcgt
      901 ttgctaacgt ggtgatgctg ctggttgccg ctggggtatt cgctcagggg cttagcacca
      961 tcggctttat tcaaagtctg atttctatcg ctacctcgtt tggttcggcg agtatcatcc
     1021 tgatgctggt attggtgatt ctgacaatgc tggcggcagt cacgaccggt tcaggcaatg
     1081 cgccgtttta tgcgtttgtt gagatgatcc cgaaactggc gcactcttcc ggcattaacc
     1141 cggcgtattt gactatcccg atgctgcagg cgtcaaacct gggtcgtacc ctatcacccg
     1201 tttctggcgt agtcgttgcg gttgccggga tggcgaaaat ctcaccattt gaagtcgtaa
     1261 aacgcacctc ggtgccggtg cttgttggtt tggtgattgt tatcgttgct acagagctga
     1321 tggtgccagg aacggcagca gcggtcacag gcaagtaaat agtaatgccg gaggcaagtt
     1381 tcctccggct ttatcatgtc acccactgtg gtatatgcgt tgtggtctgc caactttgcc
     1441 gtgaacaatt tcagcaataa tcagatggcg gctggcgcaa tattcaagat aacgcctggc
     1501 agtggtgcag ctgatggtca gtgcctgcgc caccgtttct gccgtatgtt gcacaccagg
     1561 ctctttaaac agttttcgca ccgcgtttag cgtcaagggg tcaatgccgg tcggtagctc
     1621 gtccttaggt tcaccgcgag cataagcatt aaacatctca tcaatttgct tctggctggc
     1681 gctatcaata ctttccagca tatgtttacg ctggcggaaa cgggttagcg tttgccccag
     1741 ccgttcatag gcaatgggct taatgagata atcaaataca ccacaacgta cggcttcaga
     1801 caccgtttcc atatcgctgg ctgcagtggt aaacaccacg tcgccgggat aatgcgcctg
     1861 caccagttca tgcagtaaat taatccctct accgtcagga agatagttat cgagcaagat
     1921 tagccccggc ttaaaacgct cgatcatcat tcgggcctgc gccagatttc ccgccagtaa
     1981 tatctgactg aatccgggaa tgtgacgaat atattccgca tgcatctctg ccagcggcgt
     2041 ttcgtcctca acgatcaata gggttaatgg agctgtcatt aggtttcact ttcggaatat
     2101 agattgaaaa taaggtaccg cagggatcat tatcttcgag agtgataaca ccaccgcagc
     2161 gcgttacgta gctggcaatc aagtacaacc caatgccatg ttcaccgggc tcgtcagcac
     2221 gcgtactgac cccctgctca aatattttgt ctcttagaga ctctggaacg ccgcagccct
     2281 gatcggcgac ttcaatcacc acatcatcgc cttcatcgct gaggaataat tcaacgatct
     2341 tgtttccttc atcgctacgc aggctggctt cgaaggcgtt atcaagtaaa ttgccaacaa
     2401 tggcggcaaa ctcggtgcta tccagtcctg gcggcagttg cgaaagctgg ctaccgggga
     2461 cgatgatcat ttttagcccc agttcgcggg cgcgctgcac tttaccaaaa agcagccccg
     2521 ccacctggcg atcggcaaac gcctcacgca ggctgtcaat aagctgttgc tgggcctgag
     2581 actccccctg caccatcgcc agcacgcgat catactcttt catctgcaac agaccattga
     2641 gcgtcgacat ccaattgtga tgctcgtgtc gcaatgtacg aaggctctcg acgtattgtt
     2701 ttatttgcgt cagttgcgca ttgagggtgg aaatttcgtc tttactacga aagctgataa
     2761 tggcccccag caaatcatca cctgaacgaa tagcttcccg gttggcaata acgctcagac
     2821 cattaaagtt cgccaccaca tcctgacgtt tttcatcaat ctgttcggta aagaaatcgg
     2881 cgggcctgac cacttcagca atgggtttac ccaaccattg ccgtccggga gagctcagcc
     2941 ccagcatctt tcttgcgtta cgattgatgg cggtaatgta accatgcgga tccaccgcaa
     3001 tcagcccttc ataaaccgaa ctaaacagcg cctcttgctg acggaccacg cgtgcgattt
     3061 gctttggctc catgcccatc atttgtcgac ggatatgcgc ggccaggaac cacgacagca
     3121 acatcagaat ccctaacagc acgacaaaca cgcctgccat cggtaataaa aactcagccc
     3181 gccagctatc gattttactc accaggtagc caatcgacac cacgccgatg acttttccat
     3241 cgtcatcaaa gattggcgtt ttggcgcgca tcgccatccc cattgcccct ttcccggtaa
     3301 tgaagtagct ctcccctttc tccagcgcgc cctgtttggt gaactgcata ggataaccaa
     3361 ttttctccgg attaagatgg taaaggcgga tcgagtgccg gtcgccaatc accacataat
     3421 caaaatcggt atctctttgt aatttgttag cgatggtcgc cagccgtttg tagtcacgcg
     3481 ttttcaccgc agagatgaca ctgtcattgg aggcaataat tttcgcctga ttcattgcca
     3541 tgtcgcgtac atgaagcgtt aaatagtcct caaaactggc cgtaaaatat tgcgccaggg
     3601 ctgcaatgac aaatattgag aacaccagaa tcagcaaaaa gatacgcagc ggaaatgcca
     3661 gtctttggaa aaatgcaaac tgtttattct cgttaagctg caacattgtt ttccttgcgg
     3721 gttgtcccga gcctctttga cggatgtaat taaaatgttg aatcaaaata catagatatt
     3781 aattatgatc gtggcaaatt ttattcatta aatcaattaa atcacttaaa cctgcaggtc
     3841 atttcgtaat tacattaatt aaaaaactta aaaccattaa ataaataaaa aaccactaac
     3901 tctatgtgaa ataaatcaaa atttcacgct gcaatactcc ttagaatgta tagcgaaagg
     3961 agaaaaagat atacctcgat cacccccctt tctcccaagt gaaaataaaa ggttatcaac
     4021 ttgcaacatt gaacaacatt cgttgcaaat cgataacaac atgcaccttc aggatactat
     4081 ttattatgtt cggcaatgat attttcaccc gcgtaaaacg ttcagaaaat aaaaaaatgg
     4141 cggaaatc
//
LOCUS       DAJVQG010000223         4112 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJVQG010000223 DAJVQG010000000
VERSION     DAJVQG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.226"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..3122
                     /locus_tag="OQM83_003817"
     CDS             <1..3122
                     /locus_tag="OQM83_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001745227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1521942.1"
                     /translation="RVTEQVNPEGLDYRFEYGESRVIITDSLNRREVLYTEGEGGLKR
                     VVKKEHADGSITRSEYDEAGRLKAQTDAAGRRTEYRLHMASGKLTSVVLPDGRTVRYG
                     YNNQLQLTSVTYPDGLRSSRKYDRQGRLAEETSRNGNITRWFYDSSRSGLPCAVEDGT
                     GVRRRITRNRYGQLQAFTDCSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVS
                     QRDAQGRETRYEYSAAGDLTAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAG
                     RITVLTNENGSQSTFRYDPVDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHY
                     DASDRITRRTVNGEPAEQWQYDDHGWLTEISHLSEGHRVAVHYGYDDKGRLTGERQTV
                     ETPETGEMLWEHETGHAYSEQGLATRQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEY
                     TRDRLHRETARSFGGEAYELATAWNTSGQLRSRHLNLPQLDRDYDWNDNGQLIRISGP
                     QESREYRYSDTGRLTGVHTTAANLDIDIPYATDPAGNRLPDPELHPDSTLTAWPDNRI
                     AEDAHYVYRYDEYGRLAEKTDRIPEGVIRMHDERTHHYHYDSQHRLVFYTRIQHGEPQ
                     VESRYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTIQTGTTRIQ
                     TVYQPGSFTPLLRIETENGEQAKARHRSLAEVLQEDTGVTLPAELSVMLGRLERELRA
                     GAVSAESEAWLAQCGLTAEQMAAQLEAEYIPERKLHLYHCDHRGLPLALISPEGETAW
                     QGEYDEWGNLLGETSAQHLQQSLRLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIG
                     LRGEWNLYKYPLNPVRFIDSLGLKFHVNGDPSDFNQAVEYLKQDSQMKETIDFLSSSE
                     ETINIEYIEGTNVRFNSNNMTIYWNSRASLFCSTELNSKSQSPALGLGHEFTHAQYCL
                     LDKENFMALLSRTDKKYENKEEARVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQ
                     TMKITGTPE"
     gene            3134..3583
                     /locus_tag="OQM83_003818"
     CDS             3134..3583
                     /locus_tag="OQM83_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521943.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIRWGGSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
     gene            3845..>4112
                     /locus_tag="OQM83_003819"
     CDS             3845..>4112
                     /locus_tag="OQM83_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCS1521944.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHEC"
BASE COUNT         1096 a          892 c         1267 g          857 t
ORIGIN      
        1 gtcgggtgac ggagcaggtc aacccggagg ggctggacta ccgctttgag tacggagaga
       61 gccgggtaat catcacggac agcctgaacc ggcgggaggt gctgtacacg gaaggcgagg
      121 gtggcctgaa gcgggtggtg aagaaggagc atgccgacgg gagcatcacc cgcagcgagt
      181 atgatgaggc ggggaggctg aaggcgcaga cggatgcggc ggggcggcgg acggagtacc
      241 ggctgcatat ggcgtctgga aagctgactt cagtggtact gccggacggc aggacggtac
      301 ggtatggtta caacaaccag ttgcaactga cgtcagtgac atatccggat ggtttacgca
      361 gcagccggaa atatgaccgt cagggcaggc tggcggaaga gacctcgcgc aacggtaaca
      421 ttacgcgctg gttttatgat tcttcccgca gtggcctgcc ctgtgcggtg gaagacggaa
      481 ccggggtgag gcggagaata acgcgaaacc ggtacggtca gcttcaggcg tttacggact
      541 gctcggggta cacgacgcgg tatgagtatg accggtacgg tcaacaaatc gccgttcacc
      601 gggaagaagg catcagcact tacagcagtt ataacccgcg cggtcaactg gtcagtcaga
      661 gggatgcgca ggggcgtgaa acccgttatg agtacagcgc cgcaggagac ctcaccgcta
      721 tcgttgcccc ggacggcagc cgcagtgaga tacagtatga tgcgtgggga aaggccgtca
      781 gcaccacgca gggcggtctg acgcgaagca tggggtatga cgccgccggg cgcatcactg
      841 tgctgaccaa cgagaacggc agccagtcca cgttccggta tgacccggtc gacaggctga
      901 cggagcagcg cggctttgac ggccggacgc aacgttacca gtatgacctg accggaaaac
      961 tcacgcagag tgaagatgag gggcttatca ccctctggca ttacgatgcg tcggaccgta
     1021 tcacccggcg cacggtgaac ggtgaaccgg cggagcagtg gcagtatgac gaccacggct
     1081 ggctgacaga aatcagccac ctgagtgaag gccaccgtgt ggcggtccac tacggctatg
     1141 acgataaagg ccgtctgacg ggcgaacggc agacggtgga gaccccggag acgggggaga
     1201 tgctgtggga gcatgagacg gggcacgcgt acagcgaaca ggggctggcg acccgtcagg
     1261 agccggacgg cctgccgccg gtggagtggc tgacgtatgg cagcggttat cttgcgggga
     1321 tgaagctggg cggaacaccg ctggtcgagt acacgcggga ccggctgcac cgtgagacgg
     1381 cccgcagctt cggcggggag gcatatgaac ttgccaccgc ctggaatacc agcggccagc
     1441 tccggagcag gcacctgaac cttccgcagc ttgaccgtga ctacgactgg aacgacaacg
     1501 gacagctaat ccgcatcagt ggcccgcagg agagccggga gtaccgttac agcgacacgg
     1561 ggaggctgac gggcgtccac accacggcag cgaacctgga tatcgatatc ccgtatgcaa
     1621 cagacccggc aggaaatcgg ctgccggacc cggaactgca cccggacagc accctcacgg
     1681 cgtggccgga taaccgcatc gcggaagatg cgcactatgt ctatcgctac gatgaatacg
     1741 gcaggctggc ggagaagacg gaccgtatcc cggaaggggt tatccggatg cacgacgagc
     1801 gcacccacca ctatcactac gacagccagc accgcctggt gttctacacg cggatacagc
     1861 acggcgaacc acaggtggag agccggtacc tctatgaccc gctgggccgc cggacgggaa
     1921 aacgggtatg gcggcgggag cgtgacctga cggggtggat gtcgctgtcg cgtaaaccgg
     1981 aggtgacatg gtacgggtgg gacggtgaca ggctgacgac gatacagacc ggcaccacgc
     2041 gtatccagac ggtataccag ccggggagct tcacgccgct cctgagaatc gaaacagaga
     2101 atggcgaaca ggcgaaggcg cggcaccgga gcctggcgga ggtgttgcag gaggacacgg
     2161 gtgtgacgct accggcggag ctgtcggtga tgctggggag gctggagcgg gaactgcgcg
     2221 cgggagcggt gagtgcggag agtgaggcat ggcttgcgca gtgcgggctg acggcggagc
     2281 agatggccgc gcagctggag gcggaataca tcccggagag gaaacttcat ctttaccact
     2341 gcgaccaccg gggactgccg ctggcgctca tcagcccgga aggggaaacg gcgtggcagg
     2401 gggagtatga cgaatgggga aacctgctgg gcgaaaccag cgcgcagcac ctccagcagt
     2461 cactccgtct gccggggcag cagtatgatg aggagtcggg gctgtactac aaccgcaacc
     2521 ggtactatga cccgttgcag ggaaggtata tcacccagga tccgataggg ctgagaggtg
     2581 aatggaatct gtataaatat ccattaaacc ctgttcggtt tatagattct ttgggtttaa
     2641 aattccatgt taacggtgat ccaagtgatt ttaatcaggc tgttgagtat ttaaaacaag
     2701 attcacaaat gaaagaaact atagattttt tatcaagctc tgaagaaact ataaatatag
     2761 agtatattga aggaaccaat gttagattta attcgaataa tatgacgata tattggaata
     2821 gtagagcatc tttattttgt tcaactgaat taaactcgaa atcacaatcg cctgcattag
     2881 gtttgggaca tgagtttact catgcgcagt attgccttct tgataaggaa aatttcatgg
     2941 ctcttttaag ccgaacagat aagaaatatg agaataaaga agaggcaagg gttattacaa
     3001 taattgaatc gcgtgcggcg aaaactttag gtgagtgtac cagaggagct catagtggac
     3061 tgccttttta tcgggtggat ggaccgttgc agacgatgaa aataacgggt acgccagaat
     3121 agtgaggttt tttatgatga aatatttgat ggttttattg tctctatttt ctggttcggt
     3181 attggggatg gggcgagtga atgaattgtg tggaatagat agcgtgaaaa cgatagagat
     3241 aattaatctt ccatcgtatg ttaccacgtt agttccgctt agcaaggaag gtcttaatga
     3301 aatatacaga tataaagtgg tggtgaatga aattagagat ctttatgctg gtaagataat
     3361 tgatttattg caaatgaagt atttcagaaa agaaaaatat aacaatataa gatggggggg
     3421 tagtatcatt tcgaaaggta ataataaatg tgaaatttat tttgatgctt ttggtgaatg
     3481 tggaagtgtg aatggcatta acgtgtgttt tgaaaaaaat gaaatgatag gttggattaa
     3541 aaaagaaatc cctttgctgt cgcaaaaaat aggtgggctt taaatggtag ggcaatattc
     3601 aaagacagta taggtaacta tttgtatggc catgtaaggt aaaagaaaag gaataaacca
     3661 tgagcggaaa accggcggcg cggcagggcg acatgacccg cagggaaaga tcatcagaag
     3721 ttcacttttt gtactaaata attcgcattt tatgtttaaa aattgagata ttccttatta
     3781 cctaaagctg ttttttattg cttatacatg atcaaatact tcttacataa ttaaggagaa
     3841 caaaatggaa cttaaaaaat tgatggaaca tatttctatt atccccgatt acagacaagc
     3901 ctggaaagtg gaacataaat tatcggatat tctactgttg actatttgtg ccgttatttc
     3961 tggtgcagag ggttgggaag atatagagga ttttggggaa acacatctcg attttttgaa
     4021 gcaatatggt gattttgaaa atggtattcc tgttcacgat accattgcca gagttgtatc
     4081 ctgtatcagt cctgcaaaat ttcacgagtg ct
//
LOCUS       DAJVQG010000224         4111 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJVQG010000224 DAJVQG010000000
VERSION     DAJVQG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.227"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..2162
                     /locus_tag="OQM83_003820"
     CDS             <1..2162
                     /locus_tag="OQM83_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCS1521945.1"
                     /translation="GTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKAS
                     GMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMRE
                     MMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKP
                     APYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSS
                     VWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYE
                     IDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENV
                     IAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFG
                     AGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASE
                     LDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVC
                     SKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAG
                     NVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLAN
                     IAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIH
                     EDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(2396..3679)
                     /locus_tag="OQM83_003821"
     CDS             complement(2396..3679)
                     /locus_tag="OQM83_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCS1521946.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3814..>4111)
                     /locus_tag="OQM83_003822"
     CDS             complement(3814..>4111)
                     /locus_tag="OQM83_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1521947.1"
                     /translation="LDKCKEILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEG
                     DPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT          990 a         1024 c         1091 g         1006 t
ORIGIN      
        1 aaggcactat tgcctggtct attctctctt ctcataatac gtccggaaat atggataaac
       61 ttaaaattaa gtttgattca ttagcctctc acgatattac ctttgttggt attgtacaga
      121 ccgctaaagc gtccggaatg gaacgtttcc cgctgccgta tgtgctgacc aactgccata
      181 actcactctg cgccgtcggc ggcaccatta acggtgatga ccatgttttt ggtttatcgg
      241 cagctcagcg ttatggcggt atttttgtgc ctccgcatat tgcggtcatc catcaatata
      301 tgcgtgagat gatggcaggc gggggcaaaa tgatcctcgg gtcagacagc cacacccgtt
      361 acggtgcatt agggacaatg gcagtcggtg agggtggcgg tgagttggta aaacagctgc
      421 ttaatgacac ctgggatatc gactatccgg gcgtggttgc ggtgcatctg accggaaaac
      481 ctgcgccgta tgtggggcca caggatgtgg cgctggctat cattggcgcg gtgttcaaaa
      541 acggttacgt caaaaacaaa gtcatggagt tcgttggacc gggcgttagc gcgctctcta
      601 ccgatttccg taacagcgtt gacgtgatga ccacggaaac cacctgttta agctccgtct
      661 ggcaaactga tgaagaagtg cataactggc tggcgctgca cggtcgcggc caggattact
      721 gccagcttaa ccctcaaccg atggcgtact acgatggctg catcagcgtt gatttaagcg
      781 ccatcaaacc aatgattgcg ctgccgttcc acccgagcaa cgtgtatgaa atcgacacac
      841 tgaaccagaa cctgaccgac attctgcgtg agattgaaat tgagtccgaa cgcgtggcgc
      901 acggtaaagc caaactctcg ctgctggata aagtggaaaa tggtcgcctg aaagtgcagc
      961 aggggattat cgcgggctgt tctggcggta actacgaaaa cgtcatcgcg gcggcgaatg
     1021 cactgcgcgg tcaatcctgt ggcaatgaca ccttctcgct ggcagtttac ccgtcatcac
     1081 agccggtgtt tatggatctc gccaaaaaag gtgtggtagc agatttgatt ggcgcaggcg
     1141 caatcatcag aaccgcgttc tgcggcccat gctttggcgc gggcgatacg ccaatcaaca
     1201 acggtttgag tattcgccac accacgcgta acttcccgaa ccgcgaaggc tctaagccag
     1261 ctaatgggca gatgtcagcg gtggcgttga tggacgctcg ttctatcgct gcgactgcgg
     1321 caaacggtgg ctatttaacc tctgccagcg aacttgattg ctgggacaac gtgccggagt
     1381 acgccttcga tgtaacgccg tataaaaacc gtgtttatca gggctttgtg aaaggggcaa
     1441 ctcagcaacc gctgatttac gggccgaaca ttaaagactg gccggaattg ggtgcgctga
     1501 ctgacaatat cgtcctgaaa gtgtgctcga agatcctcga cgaagtgacc accaccgacg
     1561 aactgattcc ttccggtgaa acctcttctt atcgttcaaa tccgattggt ctggcggagt
     1621 ttaccctgtc tcgccgtgat cccggttatg ttggcagaag taaagcgact gctgagctgg
     1681 aaaatcagcg tctggcgggg aatgtcagcg agctgacaga ggtgtttgcg cgcattaagc
     1741 agattgctgg tcaggagcat attgatccgc tgcaaactga aattggcagc atggtctatg
     1801 cggtgaaacc aggcgatggt tctgcgcgtg aacaggcggc gagctgccag cgtgtgattg
     1861 gcggtctggc gaatattgcc gaggagtacg cgactaaacg ctaccgttct aacgtcatca
     1921 actgggggat gttacctctg cagatggcgg aagtgccaac ctttgaagtg ggggattaca
     1981 tttacatccc tggcattaaa gcggcgctgg ataatccggg tacgacgttt aaaggttatg
     2041 tgatccatga agatgcgccg gtaacggaaa ttacgctcta tatggaaagt ctgactgctg
     2101 aagagcgcga gattatcaag gcgggtagtt tgattaactt caataaaaac cgtcagatgt
     2161 aaaaagcgcc atgtgaatgt aggtcgcatt cggcacttat tgtcggatgc gatgcttgcg
     2221 catcttatcc gacctacgaa tcgcatcgaa tctgtaggcc agataaggca tttacgcagc
     2281 atccggcact tattgtcgga tgcgatgctt gcgcatctta tccgacctac aaatcgcatc
     2341 gaaccgtagg ccggataagg cgtttacgcc gcatccggca aatagttaat tgctcttact
     2401 tcttcgcctc tgcaaccact ttactaccca cgccgcggtt attgtattcc cacatgcggt
     2461 tgtagttagt gtcattcaga ttgcgctgta tttcgtcgtt atcatctacg ctgccggtat
     2521 tacccgcaaa cggacgatta gagatcaccg catcggccca cggtttagcc gtgttaaaac
     2581 cttcgttgat ggcgctatca cggatcacca cctgaccgtt ggtattggca tcaacatcca
     2641 gcgagcggcc cagttgcgcc acaccatcac cgaaagcatt gaaacggctg tttacggcga
     2701 ggaaaccgta gtacatgttg gacagcgtag ccggtgcaaa cacatacgct tcttgctgag
     2761 tacgtgagtt caccacgcgg aattcggtgt tatcgaacac cactgcgccg cgaccagaaa
     2821 cgatatccac atccccttca atgtagctgt tggtcaccag cgtacgcggc tgacgattcg
     2881 tttccagacg gttctgcaca ccgctgttgg tgacaaagaa ggtgttctga cgaccgagaa
     2941 tgttaacgtt gttaatctgt acctggtcac catcagtacg cagtgccacc gccggatggt
     3001 tacctgcatc tacgctatcg cccagcgtgt tttcgatggt cagattttgc agttgcaggc
     3061 cattgttttg tgaccagaag accgcagagc agagaacacc gatactgtcg ctgcgtttgc
     3121 tctggcagct atcgtacata taccacgctg gtttacctgg catatatttg ccgcgcgggt
     3181 tgacgtcgtg acgccagtcg gcagggctca tgccaccatc aagggaaagc ccaatcttca
     3241 catcaatcgg tttttcacct gtaccgtaca gagtaattcc acccggagcg gcagggacat
     3301 ataccgttcc ctgatactca ccaggcatca cggcaatata ctggcgcttg ttggtacgct
     3361 tgataattgc cgcatctacc gccgcctgaa tcgtggtatg cgttacacct tgagtgcccg
     3421 ccgggccgac aacaaagtca ggttgcgcag gcagggtaat cggggaagga ttccacgctg
     3481 cagcacctgg tgtcagggat gcaaaatagt gttgagcatc gaaattctgc gcttcttttg
     3541 ccgacagaat cgggcgagaa gaggtaccag gcgcggtttg atcagaagga cgttgatcgg
     3601 gcggggttga gctacaggcg gtcagcgtca cgccaaaagc caatgccagc gccagacggg
     3661 aaactgaaaa tgtgttcaca ggttgctccg ggctatgaaa tagaaaaatg aatccgttga
     3721 agcctgcttt tttatactaa gttggcatta taaaaaagca tcgcttatca atttgttgca
     3781 acgaacaggt cattatcagt caaaataaaa tcattatttg atttcaattt tgtcccactc
     3841 cctgcctctg tcatcacgat actgtgatgc catggtgtcc gacttgtgcc cgagaagatg
     3901 ttgagcaaac ttatcgctta tctgcttctc atagagtctt gcagacaaac tgcgcaactc
     3961 gtgaaaggta ggtggatccc cttcgaagga aagacctgat gcttttcgtg cgcgcataaa
     4021 ataccttgat actgtgccgg atgaaagcgg ttcacgacga gtagatgcaa ttatggtttc
     4081 tccgccaaga atctctttgc atttatcaag t
//
LOCUS       DAJVQG010000225         4087 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJVQG010000225 DAJVQG010000000
VERSION     DAJVQG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.228"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            22..1104
                     /locus_tag="OQM83_003823"
     CDS             22..1104
                     /locus_tag="OQM83_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005099906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1521948.1"
                     /translation="MFRIPVNQWTLATAALGGVFLVSGLILLMNYNHPYTFTAQKAVI
                     AIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKTL
                     RAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDALV
                     KGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLIR
                     PGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGKL
                     TSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYSD
                     HFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1564..2001)
                     /locus_tag="OQM83_003824"
     CDS             complement(1564..2001)
                     /locus_tag="OQM83_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521949.1"
                     /translation="MADKAILWALISASTKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IKDKNKKIIDINSVNICSDDKNIKLRVNSTIMAIDDSIKCIDE"
     gene            complement(2090..>4087)
                     /locus_tag="OQM83_003825"
     CDS             complement(2090..>4087)
                     /locus_tag="OQM83_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HCS1521950.1"
                     /translation="DISHLSEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHET
                     RHAYNAQGLANRCIPDSLSAVEWLTYGSGWLSGMKLGDTPLVEYTRDRLHRETLRSFG
                     RYELTIAYTPAGQLQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLTG
                     VHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDHHGRL
                     TEKTDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYAEPLVESRYLYDPLGRRVAK
                     RVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVET
                     ATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWL
                     ASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLPLVLISTEGATEWCAEYDEWGNLL
                     NEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYP
                     LNPITDIDPLGLATCLYSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNNMQCKNN
                     PGCTHLQNRGPIPQGVWSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLTQSCLNA
                     FGPSLGPRFCSEGCITGSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          936 a          998 c         1025 g         1128 t
ORIGIN      
        1 ctggcgtggg cggtctttaa aatctttcgt attccggtga atcagtggac gctggcgacg
       61 gcggcgctgg gaggcgtgtt tctggtgagt ggtttgattt tgttgatgaa ctacaaccac
      121 ccttacactt ttaccgcgca aaaggcagtg atagcgatcc ctatcacgcc acaggtgacg
      181 ggaattgtta ctgaagtcac tgacaagaat aatcagctta ttcaaaaggg cgaggtgctt
      241 tttaagctcg acccggttcg ttaccaggcg cgagttgaca gacttcaggc tgacctgatg
      301 acggcgacgc ataatataaa gacgctgcgt gcgcagctca ctgaagcgca ggccaacacc
      361 acccaggttt cagcggagcg cgaccgtctg tttaaaaatt atcaacgtta cctgaaaggc
      421 agccaggcgg cggtgaatcc gttctcggaa cgtgacatcg acgatgcgcg gcaaaatttc
      481 ctcgcgcagg atgcgctggt gaaaggctcg gtggcggagc aggcgcagat ccagagccag
      541 ctcgacagta tggttaacgg cgagcaatcg cagattgtga gcttaagagc gcaacttact
      601 gaagcaaaat ataaccttga gcagactgtc attcgcgcgc cgagcaatgg ctacgttact
      661 caggtactga tccgcccagg tacatacgca gctgccttgc cgctgcgtcc ggtgatggtc
      721 ttcatccccg agcaaaaacg gcaaattgtc gcccaatttc ggcaaaactc gctgttacgt
      781 ctgaaacctg gcgatgatgc ggaagtggtg tttaacgcgc tacctgggca ggtgttccac
      841 ggcaaattga ctagtatttt acctgtcgtg ccaggcggtt cttatcaggc gcagggggta
      901 ttgcaatcat taacggtcgt tcccggcacg gacggcgtgc tgggaaccat tgaactggac
      961 cctaacgatg atatcgatgc cttacccgac ggcatttacg cccaggtagc agtttactcc
     1021 gaccatttca gccatgtttc ggtgatgcgg aaggtgctgc taagaatgac cagttggatg
     1081 cattatttgt atttggatca ttgaggggga agggggagat tatggaactt tgctccctgc
     1141 aagggctaat tatttttgca tattacttca ttggctgcga tgatttttct ttaatattca
     1201 ttatgttaat aagttctgga tgagtgtgct ctcattggat tttttatccg tcttgttctg
     1261 ccgcaggatg agactataca tcgtcgagat cgcaatgatg atatgagttg ttcctcctgc
     1321 gacggctcta atcaaaaaag agcaggagtg cttttccatc tattgcgatg atggctttat
     1381 tatctgaagg atgacagtca catttaaaaa atatttaatc tatagatcac aatgtagggt
     1441 catggaatat atgtatacaa ctattttctt cttcactttt aattatcgta ttcattataa
     1501 aactaagtta aaaaaactcc tagagagtga gttattaata tatttttaaa taaaattaag
     1561 ttatcattca tcgatgcatt ttatagaatc atcaatggcc ataatagtac tattaactct
     1621 caattttata tttttatcat cactacaaat attaactgaa tttatatcaa tgattttttt
     1681 attcttatct tttattttat ttactgtttc aatgcaatca gcggcaagtt ggagtggatt
     1741 tagatttttc agatatggtc ttatgatctt tcccttactt aataaatagc atgtatagga
     1801 ttcggaaagt ccagcatcta ttttgtacct cattatatta gataaggatg tgagaaattc
     1861 cttgttatca ttcgcagcca tatatgccag tccgagttca gcctctgcgg ccttacaggc
     1921 aaaatagctt agtgaacacg cttttcgtcc ctctttagta ctggctgata ttagtgccca
     1981 taatattgct ttgtctgcca aatctaactc tctctgaaat tgtcttgcag taactgcact
     2041 cgcagaaata gagaaaaaaa tacaattgtt aataactttg ccatattttt tagtccgtaa
     2101 ctgttaatgt gctatctggc tctgaaaata ttaactcatt taatttttgc atgtcattag
     2161 atgaaccagt tatacaaccc tcggaacaga atcgaggtcc taaagacgga ccgaaagcgt
     2221 ttaaacaact ttgtgttaaa aatccatcac gattatatgt ttctgtattt gctgacggta
     2281 ctaaacgtat accattaggt tttctattgg ttgctccagg accattaaca ttccagctcc
     2341 ataccccttg aggtattgga cctctatttt gtaagtgtgt acaaccagga ttatttttac
     2401 attgcatatt attaccatta ttaccagagg cgacaggaat agtaagcaca tcataagagt
     2461 tatcatcatt ttgcgtatct gaaacgcatg aaagcatact taaagttatg ctgtataaac
     2521 aggtcgctaa tcccaacggg tcaatatcag ttatcggatt caacggatac tgataaaaat
     2581 tccatccccc cttcagtcca atcggatcct gagtgatata tcgcccctgc agcgggtcat
     2641 aatagcggtg gcggttgtaa tacaggccgg actcctcatc atactgctgc cccggcaggc
     2701 ggataagctg ctgcagctga tgcgggttct cttcattcag caggttgccc cactcatcgt
     2761 attctgcgca ccattctgtt gccccttccg tgctgataag caccagcggc aggccgcgat
     2821 ggtcgcagtg gtacaggtgg atttttcgcg ccggcgtgta caccgggtcc atctggtttt
     2881 gcatctgctc caccgtcagg ccgcacgatg ccagccagcg gcggctttcc tcactcaccc
     2941 ggtcagccag gatttcactt tccagccggt cgagcatctg caccagcacc ggcgggaaca
     3001 ccacactgcc accgtcttcg ccgccggact gctgaagggc atccgccagg ctgcggcgct
     3061 gcgttttcgc cagctcaccg gtggcagttt cgaccctgat gagtggcgtg aagctccccg
     3121 gctgataaat cgtctggatg cgggttctgt cgttctgtat tgtggtcagc cggtcgccgt
     3181 cccagccgta ccaggtcact tgcggtttcc gtgacagcga catccagccc gtcaggtccc
     3241 gttcacgccg ccatacccgt tttgccaccc tgcggcccag cgggtcgtaa agatagcgac
     3301 tttcgaccag cggctctgca tattgtgtcc gcgtgtagtg caccagccgg tgctgactgt
     3361 cgtaatggta ccggtgggtg cgctcatcat ccgtgcggat aaccccttcc gggatgaggt
     3421 cggttttctc tgtcagtctg ccgtgatggt cataccggta aagatagtgc gcgtcacggg
     3481 cgatacggtt atccggccac atgctgaggg tgctgtccgg gtgcagctcc gggtcgggca
     3541 ggcggttccc cgccgggtct gtggcatacg ggatgcggat atccagattc gctgcggtgg
     3601 tgtgaacgcc ggtcagcctg ccggtggtgc tgtagctgta actccgggtc tggcgcgggc
     3661 tgctgatgcg gatgagttcg ccgttgtcgt tccaggtgta atcgcggtca gacagcaggc
     3721 tgttcaggtg ctggctctgt aactgcccgg caggggtata agcgatggtg agttcataac
     3781 ggccgaagct gcgcagcgtt tcccggtgca ggcggtcgcg ggtgtactcc accagcggtg
     3841 tgtcgccgag tttcatgcct gacagccagc cgctgccgta ggtcagccat tccacggcgg
     3901 acaggctgtc cggtatacag cggttcgcca gcccctgcgc gttgtacgca tgtctggtct
     3961 catgctgcca gagcagtgct tccgtctgcg ggtgatgcac cgtctgacgc tcaccggtca
     4021 gccggccttt ctcatcatac ccgtaatgca ccgtcacccg gtgcccttcg ctgagatggc
     4081 tgatgtc
//
LOCUS       DAJVQG010000226         4066 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJVQG010000226 DAJVQG010000000
VERSION     DAJVQG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.229"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..774
                     /locus_tag="OQM83_003826"
     CDS             <1..774
                     /locus_tag="OQM83_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS1521951.1"
                     /translation="RHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVNQQQ
                     QNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQLSDTF
                     AILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQGNRQ
                     IIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSRLF
                     IRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            787..1747
                     /locus_tag="OQM83_003827"
                     /pseudo
     CDS             787..1747
                     /locus_tag="OQM83_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(1785..2684)
                     /gene="nhaR"
                     /locus_tag="OQM83_003828"
     CDS             complement(1785..2684)
                     /gene="nhaR"
                     /locus_tag="OQM83_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCS1521952.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2750..3916)
                     /gene="nhaA"
                     /locus_tag="OQM83_003829"
     CDS             complement(2750..3916)
                     /gene="nhaA"
                     /locus_tag="OQM83_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCS1521953.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1219 a          950 c          860 g         1037 t
ORIGIN      
        1 cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta ttcctccaac
       61 aatactggta ttactggcat agccggtgaa catgatcagt taaattatgg tatatatgtt
      121 aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg gagaactccc
      181 atcgccataa tagatggcag ctatagtcat tctaaaaacg cctggcaaag tggtggaagt
      241 attagtagtg gattagttgt ctggcccggt ggtattaata tcactaacca gttatccgat
      301 acatttgcaa ttctggatgc gcctgggtta gaaggtgcac atattaatgg acaaaaatac
      361 aatcgaacaa acagcaaagg ccaggttgtt tacgacctga taatacctca tcgtgaaaac
      421 catctggtac ttgatatagc aaacagtgaa agtgaaacag aattgcaggg caatcgtcaa
      481 attattgcac cttaccgtgg agcagtttct tatgtgcagt ttacaactga ccaacgtaag
      541 ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg ctatgacgta
      601 ctggatctcc aggaaaataa tattggagtc gttggccagg gtagtcgcct ttttattcgc
      661 gtagatgaaa ttcccactgg aataaaagtt gctctcaatg atgaacagaa cttattctgt
      721 actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca gtaaaaggag
      781 tttactatga agtggctttt attaatcacc ttgagtctat attcttttat tgtgcaaagt
      841 gctccctgtg cattgaccaa tgtcggggaa caacgtggaa catatatact caagccactg
      901 agtatgaaag gtaatctcac cgccgaaaaa attgaaaaca ccggaaatat cattgagtgt
      961 gcagacttac aagagacgac caacgccatt aaagttcatt ttttaacaca gaacgcttta
     1021 aggtttaaag tcggaagtcg tcattatatt attaatttcc cctttgaatc tgacggtaaa
     1081 aaaaatagag cttccaggaa ggtcatctta taatttagaa gaaatgctat ctgcgataaa
     1141 tttcaccatt ccatactcta tcgcatctgt atcagatagt tccaacaccg cagatatttt
     1201 tccaggaact ccttttcctt tgttgttcga aattgaagtg agttcatgta cagggaataa
     1261 tactaattgt aatagcgtcc gctttaatta taatttaaat actattttac aggtaaattt
     1321 aatgacctgt ggttttgaag atcaatccat cgacactgga cgatttgtac tgtctcgtat
     1381 caataatgaa aattatcagt ttcatcatat aagatttgac tgcatccagg gcgagcaagg
     1441 tgaattagtt tttgaaccca gtgatgttga atattacttt gaaccagtga cgatacagga
     1501 gtcaggaacc agcatattaa aaaatgagtt agaaaacagt gaggacggtg cgggtcaagt
     1561 aggctttcaa ccctccaatg atggtacgaa tgaaatccag tatggtaaaa gcaattacta
     1621 ctcttttcag catccccatg aaggaagtaa tcagattcct ctatttattc gcccgcgtac
     1681 atatggaaat aatgtctcaa gcgggcaaat aatgagccga gtcaaaatcg tggttatgta
     1741 caactaatct atctccccca ccttaacttt gggggaggtg ccgattaacg cgccgctgga
     1801 ctaaaaagcg cagaataatc cgtattgcag attcgctgta ccgccgggtg ctgaatcatc
     1861 cgctcagcaa aaatagcatg gtactcttcc atcacattct cgacgcgacc aatttctacg
     1921 acagttttat cggcataaaa gtcatatgca taaagcgttg gggcaacgaa gattgcattg
     1981 tgcatcgcac caaaagcttt catcaacgcg gcatcatcaa actcgccgag gatttctacg
     2041 tttaatccct gggagttaaa ccagttaagc aatttgcgcc ctaacattga acgtcgccca
     2101 ggaatcaaaa gtcgccgttc ttccagacaa gccgggaacg atttttctgg tggtggattt
     2161 gtacaccaga aactcacgcc acattcgcca attctcacgg agaacaggcc ttcctgctgc
     2221 gtagagtcta tcggacagtc agaaatgatc atatccagtt tatgctgact taattgctcc
     2281 agcagcattt cgtgggtgga ttcgaagcag cgaagatgaa tgggctcgcc ttctaccact
     2341 gcggcgttaa gtacgctact gaccaggcgt ttggaaagtg catcagccac gccaacgtca
     2401 aacaataaat tggattcttt gcgatagttc acaatatcca gcatttcctg gcttaaggtg
     2461 aacattttat cggcatagcg atagaccagt tctcccagct cgctgggttc gagaccacgt
     2521 cccttgcgtt taaataattt gccttgcagg cgctcttcca gcgctcgaat ctgtccggta
     2581 atggtttgtg gagttaaata aagcgcctcc gctgcgccaa ccacggaacc ttctttatag
     2641 acatgccaga agtaatacaa gtggttgtaa ttgatatgag acatgctcat ttctctccct
     2701 gataacaatg aaaagggagc cgtttatggc tccccggtaa accgtcctgt caaactgatg
     2761 gacgcaaacg aacgcgtaac cagctgtatc caattaccgc cgaagagata gaaccgacca
     2821 ggataccgag tttcgcccag ttaatcagtt ctggatctac gctaccaaag gccaggctgg
     2881 caataaagat agacatagta aaaccgatac cgcacaggat ccccaccgcc ataatttgct
     2941 gataagtcgt tccctcaggc agatgcgcca gtttcaaacg cagcgccaac cagcagaaca
     3001 gactaatccc cagcggtttg ccaatcagca agccagcgat gatccccaat ggcagaatgg
     3061 aggtcaagcc atccagcgtg acgccttgca gtgaaacgcc agcattagca aatgcaaaca
     3121 gcggtaaaat cagatacgcc acccacgggt gcaatacatg ctccagtcgc ttcgctggag
     3181 aacgcccatg cttctctttc aaaggaataa agaagccgac aattaccccc gccagggttg
     3241 cgtgaacccc cgatttcaac accgcagtcc acaacaccac gccaacaaga atatagacgc
     3301 ccgtgcggcg tacaccacac agattcaata ccgcgagtac cgcaattgct acagccgcga
     3361 cgccaagaga ggccatcgat aagtcattag tgtagaacaa tgcgatgata atgatggccc
     3421 caagatcgtc gataatagcc agagccatca aaaagatctt cagtgctaac ggaacacgac
     3481 ttcccaacag cgccagcaca ccaagtgcaa aggcaatgtc agtagccgcc gggatcgccc
     3541 acccttcgcg ggtaatcgga tcggcatagt taaaggccag atagagtaat gccggaacaa
     3601 tcatcccacc aatagcggcg ataaccggaa atgcggcctg gcgtaagctg gctagcgatc
     3661 cttgcatcag ttcacgttta acttccagac cgactaacag gaaaaatacc gccatcagcg
     3721 cgtcatttat ccataacagc atgtttttgt tgatttcgag tgaaccaacc cggagctgaa
     3781 ccggcgtctc cagaaagtcg tgataccatc cactggttgc gccgctgttg gccatcatca
     3841 tcgccaggat agcggcaatg ataagaataa tgcctcccga ggcatcactg ctaaagaatc
     3901 gatgcagatg tttcactttt tatttctctt tcaggtgaat agatcgtttt tactatttta
     3961 ccccgcacaa atcatcgtta aaatcagatt atagtgtagg aataactcgt tttttacgag
     4021 caattaaagg atttgcgcca tcagcggtga gatgagtttt tgacgg
//
LOCUS       DAJVQG010000227         4020 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJVQG010000227 DAJVQG010000000
VERSION     DAJVQG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.230"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            168..503
                     /locus_tag="OQM83_003830"
     CDS             168..503
                     /locus_tag="OQM83_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1521954.1"
                     /translation="MLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTED
                     TIDGLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLT
                     LSYREVNRD"
     gene            496..978
                     /locus_tag="OQM83_003831"
     CDS             496..978
                     /locus_tag="OQM83_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521955.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(1012..1092)
                     /locus_tag="OQM83_003832"
     CDS             complement(1012..1092)
                     /locus_tag="OQM83_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521956.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(1128..1552)
                     /locus_tag="OQM83_003833"
                     /pseudo
     CDS             complement(1128..1552)
                     /locus_tag="OQM83_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1982..2472
                     /locus_tag="OQM83_003834"
                     /pseudo
     CDS             1982..2472
                     /locus_tag="OQM83_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2697..3188
                     /locus_tag="OQM83_003835"
     CDS             2697..3188
                     /locus_tag="OQM83_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCS1521957.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            3523..3981
                     /locus_tag="OQM83_003836"
     CDS             3523..3981
                     /locus_tag="OQM83_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCS1521958.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDG"
BASE COUNT         1502 a          649 c          647 g         1222 t
ORIGIN      
        1 tccataatga cattaacata caaaatcatg taataaactc gtcaacagac gcacatacac
       61 acaatgcccc acctgccata aaagttaatg cgggatacaa agagagcatt ggtggcgcaa
      121 agaatttcaa taacaaaatc ctcaaacata taaaaagtca tctaattatg ttgagcgcat
      181 ttgtcatagg cgcatattct gcatattggc tatggaataa taatcaaccg aagccatttt
      241 tcaaggatta caagactgtt gcggaaatta atggctgcca ttttaatgta acagaagata
      301 caattgatgg gttgaaagag ttcgataaat ataaaacacg gatactggat tcggggatta
      361 actgtaaaaa atatccgtgg ttatacttcc ctcttgcaaa atcctcaccg gggatgattg
      421 ttatggcatg caataaaaac tataaccaac atgaagtggc agactgttta accctgtctt
      481 accgtgaggt taatcgtgat tgattataaa aaaaacttat tatttatttt agtttttatc
      541 agtgggttta ttctcttcac tgtctattct tacactgcag aaaaaatgat atataacgaa
      601 acatgcactg caaattgggt aatttttaat gaccagggac gagcaaatct taccatcgac
      661 tttatgtata acaaaaaaaa taaaacagga actgttgcgt tgagtggaac ctggcaacaa
      721 ggtaacagag aaagtaaatc aatacggcga aatattgaat acacatggat tgaaaactat
      781 gacacagccc atttaacatc aaagaaagtt aataagtttg agattatgga tcaagttgat
      841 gatgatagac ttgcacaact tattcctgat ttttatgtct ttccagaaaa aagtgtaagc
      901 tataatatac taaagcaagg taagcatgct tttattttga gcattggtaa cagagcaata
      961 atgcattgtg caaggtaaac taaaatagat aacaaaaaag atggaacctc gttaatcata
     1021 aaacaataaa attaaatatt ctcgcagtat atggcagtct aaagcatcaa agttttgatc
     1081 aacatctttc attttagaca tctccttgca atgtaaaata tcatgtatca acaacatctg
     1141 gtgcaaatcc atgagtcgaa ctcgacattc atcttagctc gattattatt ccttcgtaca
     1201 gtcgatgtaa acaatacaga aagaggatta ttaagaacca gtttactcag gaatcaatag
     1261 aggaaaggac taacgtttct ttaaaagaat tgatttcatc atctgttaaa ctaaactcat
     1321 cattgacaga tcgtgagata taactgtttt taactttact ctttacgttg actttattga
     1381 cagaattaac attcacatat cttgaattta atgtccatga tgttgtttgc gtgagaacat
     1441 tctcagcatt aaggaatttt tttaacggaa tccttggttt ctttttcgag tccgaattta
     1501 caatatcatg catatagacc atttcatgaa tctgcgaaaa ctcaatgtcc attgtatacc
     1561 tcacattttt accgtgactc gatgttactg ttcaataatc accttccatc aatactaaaa
     1621 ttaatacccc taatgtgccg ataacaaata tagtcattct acgtaacgtc tccataaggt
     1681 gataaccttg atcaaatgaa tttcgaatat aataaaggaa atcttttgag atgtttatca
     1741 cgaataaagc tatttatgtt accaatatca tttcgagtgc gcacactatc tatctgtttt
     1801 ttcgagatat aaaaaacaac gtgtaagtta tgtaaattgc cgatgctact gagtaacatt
     1861 atggtaaaaa aagtaaaaat gagcgatttt tgatagaata aagcatgaga aaaggtgata
     1921 tttgacatta tcagaagctg cgaattcgga ttttgctcta atcaaatgag agaaatgtaa
     1981 aatgaagcca cgaaatatta ataatagcct accactgcaa ccattagttc ctgatcagga
     2041 gaacaaaaat aagaaaaatg aagagaaatc cgttaatcca gttaaaatca caatggggtc
     2101 tggtttaaat tatattgaac aagaatctct tggaggaaaa tatctaacac atgatttgtc
     2161 aataaagata gcggatattt ctgaagagat aattcagcaa gcaatattat ctgctatgag
     2221 catatataaa ttttcgataa cagatgattt aatgagtatg gctgtaaatg aactcataaa
     2281 actgaccaaa atagagaata atgtagacct gaataaattc actactatat gcacagacgt
     2341 tctatccccc ctcgtcacca gacataataa agaaaaaaaa caaacgacat tctacccttc
     2401 tcaaaaatcc cctttttaat tttcattgaa aaaatggcaa ttgatactat gaatccaggt
     2461 catacacatt aaggcattta tgtaatgtcg taattaagat aactaataag gtgaatatta
     2521 gtaaaacaac taatgatgtt attagtttgt agtgaacagt acttttacca ataatgaaaa
     2581 atataccata agcaatgcaa gcttaaaact aataatttaa aaaagataaa tgtaaaaata
     2641 ttaatgagtc aatcaataat aatttagcat cacgagacac atcacagagg aatattatga
     2701 gcacagaaac aattgaaata ttcaataata gtgatgaatg ggcaaatcaa ctaaaacacg
     2761 cattatcgaa aggagaaaat ctggcattac tacatggttt aactcctgat atccttgata
     2821 gaatatatgc atatgcattc gactaccatg aaaagggtaa tataacggac gcagaaattt
     2881 attataaatt tttgtgcatt tatgcgttcg aaaatcatga gtatctaaaa gattttgcat
     2941 cagtatgtca gccaaaaaag aaatatcaac aagcatatga cctttacaaa ctaagttaca
     3001 attactcccc gtatgatgac tattcagtta tttatcgtat gggtcaatgt cagattgggg
     3061 ctaaaaatat cgataacgca atgcaatgtt tctatcacat tattaacaat tgtgaggatg
     3121 atagtgttaa gagtaaagcg caggcatata ttgaactctt aaacgataat tcagaagata
     3181 atggctaagc attacaatca tttggttaaa tttgctggtt atagtgcagt taactactat
     3241 aaccagcacc tcttatgttt tcttaaatca gcatcttaaa acagttaaga gcatactata
     3301 aatcattatt tttcaatgcg ttagagaaca atctacttta taaaaattga ataaggctat
     3361 ttcagattac atcactgaat tcctaagata aattagtgaa cacaaccata gatatgtcga
     3421 atgtattttc ctgtagcatt aatgacacat aaatatgaat agccataatt tctattgctt
     3481 aagcaggatg caagaaacca attttttcat agaggttaac taatggactt agaaaataaa
     3541 ttctcatatc attttcttga gggattaacg ctcacggaag atggaattct tactcaagga
     3601 aatgagcaag tttatattcc acagaaagag ttaggtgtat taatagtatt acttgaatcc
     3661 gctggtcatg ttgtactgaa agatatgatc atcgaatcag tatggaaaaa tattattgtt
     3721 agtgacgagt ccctgacaag atgtatctat tctttgcgct gcatttttga aaaaattggc
     3781 tatgatcgtt gcatagaaac aatctaccgg aaaggttatc gtttcagcgg gcaggttttc
     3841 aaaactaaaa ttaatgaaga taatacttca gactattcca tagctatatt ccctttcact
     3901 acttcattga atacactgga tccattaata cttaatcagg aattagtgca aatcatttca
     3961 aataaaaaaa tcgatggtta gactggcccc ctgaatctcc agacaaccag tatcacttaa
//
LOCUS       DAJVQG010000228         3993 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJVQG010000228 DAJVQG010000000
VERSION     DAJVQG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.231"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(173..2122)
                     /locus_tag="OQM83_003837"
     CDS             complement(173..2122)
                     /locus_tag="OQM83_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1521959.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2207..2542)
                     /locus_tag="OQM83_003838"
     CDS             complement(2207..2542)
                     /locus_tag="OQM83_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCS1521960.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2542..2898)
                     /locus_tag="OQM83_003839"
     CDS             complement(2542..2898)
                     /locus_tag="OQM83_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCS1521961.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2898..>3993)
                     /locus_tag="OQM83_003840"
     CDS             complement(2898..>3993)
                     /locus_tag="OQM83_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCS1521962.1"
                     /translation="ATAVATAIKEAVNGVITLPFTASSDAGVVTLTARHKGLYGNELP
                     VCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAAMGDEAFDFIGLPFNDAAS
                     INMMMTEMNDSSGRWSYARQLYGHGYTAKLGTLSELVGAGDMHNQQHITLAGYEKETQ
                     SPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKGKRFIMTEQQTLLSHGVAT
                     AYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAYVLRKLKSVITSKYGRHKL
                     ANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYDLFKQYLIVERDADNPNRL
                     NTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT          930 a         1178 c          874 g         1011 t
ORIGIN      
        1 aacgttacta ataaatcgtg aaacaatctc ctcatttgtt agtattttcg cctgagcaca
       61 gcaagaggtt aatattgcaa ccgtcattaa aaatggcttt atcacaaaac aaactccatt
      121 gttagcatgt tacgcgataa ttttgcctca tctttcacca caaaaaaaga actcaccttt
      181 taaatggaga atacccaaca tcatgggtga ttttcataaa gggatcgcct gttttcggca
      241 aatcgataac gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt tcgctgcggg
      301 ttgccggtga agatgcaagg cttaacagct gattcctttc cagtgataca ttcggggtgt
      361 agcgtttatc gggagataaa tacccatcac cacgtggcat ctgccatccg gtcatgtcat
      421 agacaaaatc atggaactgt ttgccccact cgtcgaactt atcattcaga ccaaatccac
      481 tgttcagtgc atcagccaca aaatttttaa caagccaggg atgtgctttt tcaaactcct
      541 gcgccatcat gcccagttca agcaatccac caatcagact gatctttccg aggcccttta
      601 atttcagccc tgtttttcct gccgccgttg tccattgatt ctgggcgact gttgccgcac
      661 gaatacttgc caccgccttc acaccaatat acagggcaga aaccgttgct attgttttta
      721 ctgcctgttc ccatcccccc attgctctga caacctgatc aacctcctgc cagacttttt
      781 tcacaaccgg accgaccgtt tcccagttat caattatgag atatgcacca ccgacgagaa
      841 gcgcaattaa tccctttgcc ggcgtcatat tcatcacgct acccatgatt tttgttatgc
      901 gtgtcaatgt gccaatcgcc acgcccatcg tcagtaatgc cgcaccagtt ttcgcgatgg
      961 tttttacgac ctcaggattt tctctgacaa acgtgcgcac ttcttccaga aaaggcttca
     1021 tctcttttat gccttcattg agtgaaggca ggaaggtttc ccccagcgtg gaagaaatcg
     1081 cattgatctg gttctgcagc agcaacaact gattttctgt cgtcgcggca cgggcggcat
     1141 attctttctg cattgagccg ccatactgct gcgcatccgc cacacgatta aagttggttc
     1201 gcaacaaatc cagattcgtg agcagcggcg caatggcccc cagggattcc ttcccgaaca
     1261 gcgctttcag cacagcagcc tgtttttctt tcggcacttt tgccagagaa tccagtacgt
     1321 gcagcatggc cccgcgggcg tctttctgca tatccgccgc cagttttttc gggctaatgc
     1381 gcaacgagcg gagcactttt ttctgcgatt tggtggcaga atccctcgct gtcagcgaca
     1441 acataaaatt ctttatcccc gtcgcagcaa tttctgactc cacccccatt ccggcaatgg
     1501 ttgcccccat tgcggcaatc tctccggagg ccacacccgc aacactgcct aaagggccaa
     1561 tacgggtcac aacatcagaa atctttttcg cacttgcagg accggtatta ccaagatagt
     1621 taatcttatc cgcaagccct gccacctctc cctgtgtcag tttaaaggca gtgcgccact
     1681 gcgccatcat ctgaccggat tcttctgccg tggtgtcaaa ggccacgccc atcttcacgg
     1741 catcgtcagt aaactgcatc agctcgtcac gggcgatacc agcctgacca cccgccgcca
     1801 caatttcggc gataccttcc gccgacatgg gcagttctgt tgacaggtcg cgcacctgct
     1861 ccgtcattgc cttaaacgct tccggcgtat ccagaccatc caccactttc cggacatccg
     1921 ccatttttga ttcaagggca atggcagatt tgaccgggag cgccagcgcc cccagtactg
     1981 cggttccggc acccgctgcg cccagtaaca ggctggcaaa ctccttttta aaacctttca
     2041 gctggcgctg cattcccttc agcggtgccg ataccttatc cacggcagtg atgatcgcct
     2101 tcagctgaaa actgtcagcc atgcttcatc tcctcgttaa tgcggacggc ctcggcttcc
     2161 agttctgtaa actgcgaaac agccccccgt cgcagctcca gtggattcag tttccagaac
     2221 caggcaacat tgtagagtcg tttccggagg tgcttcccgt ctccgactgg gtaaaaaaac
     2281 gcaggatctg catgctggtc ttaaaaatat ccagttttgc catctgcgcc gccgatgaac
     2341 gcgggatccc cgccagcaac gggatatatt tcagtgccac ctggctgtcc agtttaatac
     2401 tgccttcacc ggaaataatg aaaggaaaac ccagcgcctc gatttcgtca tacgtgggct
     2461 cgcgcaactc cagcacatgc agcatttcgt tatgcgctgt caccggtttt tttaaaacta
     2521 tttctgtaac gttcattgcc attactgata ccctccctct tcgccgtgga attcaagatc
     2581 tgccgtgcct tcttcggcat tatgattagc ctctccgtgc agccatgccg ccgaaagcac
     2641 atacaccatg ccgtttgcca gctcagcggt aatcgtcatc tggtctgagg tggtaatttt
     2701 gtcgaccgga aagtttttgg gcactttaaa cgtgccctta acgtaaggtg accgccaggt
     2761 ctccttgtaa tccacatccc ctgccatgcc gacgatgtca tcccggacat tggtgttcat
     2821 cggcacttca atgccacctg tcagcgataa ctgctgacca tccactttaa aaaaacaggt
     2881 tccggcgatc tttgccatta tgctgactcc tctgaatact gaagacggaa ctggttaacc
     2941 accgcaaaga cacgtaactg attaacataa tccggcggga acagcgtatt cagtcggttc
     3001 gggttatctg catcacgctc aactatcaga tactgtttaa acagatcgta attttccaca
     3061 ataccggcac gctccatctg acgatatgtc gccagaagtt cccctttgat aacggcagga
     3121 gtgacaatcg cctgccccgg accaaaacgg gtaccatcat ttgccagctt gtgacgtccg
     3181 tacttgcttg tgatgaccga tttcagtttg cgaagaacat atgcactggt atgcagagtt
     3241 tcactgtcca gatagctgtt gtctgccacg ccatacgcat tctttttgta ggtggttacg
     3301 gaacgctgga tctgcaacgt gccgccttcc acataagccg tcgccacgcc gtgagataaa
     3361 agggtctgct gctctgtcat gatgaatcgc ttacctttcg gtgccggaag catccccacc
     3421 agctcccccg tctgtgtcgg acgggcagga tcattccgga taaataccgc ttcacgggca
     3481 aggcgactgg caaccagttc atcgacaggc gactgggttt ctttttcgta accggcaagc
     3541 gtgatatgtt gctgattatg catatctccg gcaccaacca gctctgacag cgttcccagt
     3601 tttgcggtat agccatgccc gtataactgg cgcgcataac tccagcgacc gctgctgtca
     3661 ttcatttcgg tcatcatcat attgatggag gcggcatcgt tgaacggcag accgataaag
     3721 tcgaatgcct catcgcccat agcagcaaca gcggcggtaa gatcaggcgc tccgctaccg
     3781 gcagcaccga cttccgtcac cacccgaagc cctgctggca gaatctcacc accaccagaa
     3841 ccataataat tcagacagac cggtaactca ttaccataca gccccttatg acgggcagtc
     3901 agcgtcacca cacctgcatc agatgacgcc gtaaacggca gggtgataac cccgtttacc
     3961 gcttccttaa tcgcggtggc aaccgcagtg gca
//
LOCUS       DAJVQG010000229         3959 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJVQG010000229 DAJVQG010000000
VERSION     DAJVQG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.232"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..242)
                     /locus_tag="OQM83_003841"
     CDS             complement(<1..242)
                     /locus_tag="OQM83_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1521963.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="OQM83_003842"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="OQM83_003842"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCS1521964.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2255..3904
                     /locus_tag="OQM83_003843"
     CDS             2255..3904
                     /locus_tag="OQM83_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCS1521965.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1031 a         1001 c          927 g         1000 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtaacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccacc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 tagacgccga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1021 tcccaagatt tcgacgccat ttcaatcggg ttcaacggcg gtccaccagc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1381 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gaagcgcggt ataacgcgtt ggcggcatag tttcatactg catcacccat
     1501 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggcg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaaacctaa aaccttatta ttttcactga cagcggatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagtgagat acgctttttg ataggaatca atcaattaca
     1981 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     2041 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2101 tttttataaa ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     2161 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2221 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     2281 taatccagac gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt
     2341 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2401 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2461 gaaaattttc ccggacagta aaacctttcc cgactgcgca cctaaaatgg acccgctgga
     2521 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2581 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2641 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2701 cattccgtgg tcttctctac tggcgctgcc gcagtcatat attgtcccag gcggccgttt
     2761 tagcgaaacc tactattggg actcctattt caccatgctg gggctggcgg aaagtggtcg
     2821 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2881 catccccaac ggcaaccgca cctattattt gagccgatcg caaccaccgg tttttgcgct
     2941 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     3001 taaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     3061 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttactggg acgatcgcga
     3121 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     3181 gcccaacgag gtgtaccgcg atttacgcgc gggggcggcc tccggttggg attactcttc
     3241 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     3301 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3361 aggcgagaaa gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3421 aaaccgttac ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga
     3481 acaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca
     3541 tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat
     3601 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaacggct gggcaccgtt
     3661 acaatggatg gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     3721 cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga
     3781 gaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     3841 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     3901 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagg
//
LOCUS       DAJVQG010000230         3859 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJVQG010000230 DAJVQG010000000
VERSION     DAJVQG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.235"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            116..511
                     /gene="caiF"
                     /locus_tag="OQM83_003844"
     CDS             116..511
                     /gene="caiF"
                     /locus_tag="OQM83_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCS1521966.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(720..1310)
                     /gene="caiE"
                     /locus_tag="OQM83_003845"
     CDS             complement(720..1310)
                     /gene="caiE"
                     /locus_tag="OQM83_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCS1521967.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(1316..2101)
                     /gene="caiD"
                     /locus_tag="OQM83_003846"
     CDS             complement(1316..2101)
                     /gene="caiD"
                     /locus_tag="OQM83_003846"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCS1521968.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFDGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            complement(2210..3763)
                     /gene="caiC"
                     /locus_tag="OQM83_003847"
     CDS             complement(2210..3763)
                     /gene="caiC"
                     /locus_tag="OQM83_003847"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCS1521969.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
BASE COUNT          911 a         1050 c          942 g          956 t
ORIGIN      
        1 cttatcgacg gtgaagttgc atactatcga tatatccaca attttaatat ggccttgttt
       61 aattgcttca aaacgagtca tagccagact tttaatttgt gaaactggag ttcgtatgtg
      121 tgaaggatat gttgaaaaac cactctactt gttaatcgcc gaatggatga tggctgaaaa
      181 tcggtgggtg atagcaagag agatctctat tcatttcgat attgaacaca gcaaggcggt
      241 taataccctg acttatattc tgtcggaagt cgcagaaata agctgcgcag ttaagatgat
      301 ccctaataag ctggaaggac ggggatgcca gtgccagcga ctggtaaaag tggtcgatat
      361 cgatgagcaa atttacgcgc gcctgcgcaa taacagccgg gataaattag tcggcgtaag
      421 aaagacgccg cgtattcctg ccgttccgct cacggaactt aaccgcgagc agaagtggca
      481 gatgatgttg tcaaagagta tgcgtcgtta atgtcatttc gttgatacca cgcagcctgg
      541 ttgctggatg caatgctggc gcatcttatc cagcctacgg ttgctctcca gccgggttgg
      601 ataaggcgtt tatgccgcat ccgacagcta tcgccggatg cgatgctggc gcatcttatc
      661 cggcctacgg ttgctctcca gcagagttgg ataaggcgtt tacgccgcat ccggcaagat
      721 caccgtttag gcgtcacatc cgtcgtcccc tgcaaacggg ggcgattttc ctccatttgc
      781 ctcagtggct gtgtttcatg taacgatgca tgacagcgcc cgacaagatc ctgatactct
      841 ttggtattca accgtttcca gtgtaactcg tcgtcactaa cactgcgtac agcgcgggct
      901 ggcgtaccca tcaacaactg gcgtttctcg ccgtgaaagc ctgctttgac aaagctcatg
      961 gcggcaacaa tgctctcttc gccaatgacc gcgccatcca taatcacgct gttcatcccg
     1021 accaatgcat cgcgaccaat cacacaacca tgcaggatcg ctccgtgccc gatatggccg
     1081 ttttccccaa cgatggtgtc agtgtcgcag tagccatgca taatgcagcc atcctgaata
     1141 ttggctcccg cttgcacgat caaccgcccg tagtcaccac gcagtgaggc gagtgggccg
     1201 atgtagacac cggctcccac aatcacatcg ccaatcaaga cggcactggg atggacaaac
     1261 gccgtcgggt gaaccaccgg aattaacccc tcaaaggcgt aatagctcac ggttgttaac
     1321 gtcctttcca caccggatcg cgcttctcgg caaacgccag cggcccttca atggcatcct
     1381 ccgaatgcag aaccgacgga tagtgtttca acacgccgct gcgaatatag cgatacgctt
     1441 cttctaccgg catttcgctg gtggtgcgga agatctcttt cagcgccgca atcgccagcg
     1501 gggcgctgtt aaccagctgc tgcgccagtt cgcgggcgtt atccatcagt tccgcctggc
     1561 taaccacgcg gttgactatc ccccaacgca gcgcctcttc tgcgcccatt cgtctgccgg
     1621 tcatcaccat ttcattgacg atggcaggcg gcaggatctt cggtagacgc agcacgccgc
     1681 cgctgtcagg aacgatgccc agcttggctt ccggcagggc gaagctggcg ttatcggcac
     1741 aaacaataaa atctgccgcc agcgccagtt cgaaaccgcc gtcaaaggca tagccgttca
     1801 cagctgcgat aaccggtttg tcgagattga aaatttcggt taatcccgca aatccacccg
     1861 ggccaaagtc agcatccggt gcttcgcctt ctgctgccgc ttttaaatcc cagcccgcgg
     1921 aaaagaactt ctctccggca ccggtaataa tggcgacacg taattgcgga tcgtcacgga
     1981 aatttagaaa tacttcgccc atttcaaagc tggtttttgc atcaatagca ttcgcttttg
     2041 gacgatcaag ggtaatttcc agaattgatc cattgcgggt cagatgtaat gattcactca
     2101 ttccttttct ccatttttgc tttttcaggg acgacaacat ccctgcaaaa aatgcatatt
     2161 gttttatagc gtgattatta gttggcaggg tagttccctg ctgtttcatt tatttcagat
     2221 tctttctaat tattttcccc gagcaattac gtggcagatc ttttctgatc tccagataag
     2281 agggcacttt aaatttcgcc atattttgtt cgcagaagcg gaaaaattcc tcttcgctca
     2341 atgtttcacc ttcattcagc accacaaatg ctttgatggc ttcatcgcga atcgaatctt
     2401 taatacccac aaccacgata tcctgaattt ttggatgggt ggcgatgata ttttccagct
     2461 ccacgcagga gacattctcg ccaccgcgtt tgatcatatt gcagcggcga tcgacgaaat
     2521 aaaaaaagcc ctcttcgtcg cagtatccgg tatcgccggt atgtagccag ccgtcggctt
     2581 ccagcacttt cgcagtggct ttcgggttga gaaaatactc tttgaagatg gttttccctg
     2641 gtacgccttt aatacagatt tcaccgatct cacctgccgg gagcgggcga ttgtgatcgt
     2701 cgcggatctc cgcttcgtag caaaaccccg cccgaccaat cgacggccag cgtcgtttat
     2761 cgccagggcg atcgccgata atgcccacaa tggtttccgt catcccataa gacgtcagca
     2821 agcgaacgcc gaagcgttca caaaacgcat ctttttcctg ctccgacaag ttgagataaa
     2881 acatcacttc ccgcaggcgg tgttgccgat cgttcgctga aggcggctgc gccatcaagg
     2941 tacggatcat catcggaata cattcggtaa cggtggcgcg gtacttctgt acctgtcccc
     3001 agaaggcgcg ggcgctgtat ttctcgacca gcacaaaggt ggccccggca gaaaacgccg
     3061 ccatcgccgc agtacactgg caatcgatat gaaacgcagg cattaccgtc aggtagacgt
     3121 catcgtcacg cagtgcacac tgccaggcgg agtaatatcc agcgaagcgc aggttgtaat
     3181 gggtaatcac cacacctttc ggtcgggagg tggtgccgga ggtgaagaga atttccgccg
     3241 tatcgtcagt cgatagcggc ggtgcatagc acaaggtggc aggttgttga tttttcagtt
     3301 gagtaaacga actcacgcca tcatcagcgg gaagtgccac atctgtcagg caaatgtgcc
     3361 gcaattgagt ggcatcttcc tgctgaatct gttgatacat aggatagaat tgcgcactgg
     3421 tcaccagcag gcacgcctgg ctattttgca ggatccacgc gctttcctcg cgcaacaggc
     3481 gggcgttaat cggcaccata atcgcgccaa tttttgccag cccgaaccag caaaagataa
     3541 attccgggca gttgtcgaga tgtagagcaa ccttgtcgcc tttgcgaatc cccagcgtat
     3601 aaaacaggtt tgtcgtgcgg ttaatctcct gatttaactc aagataacta taccggttaa
     3661 cgactccgcc gctggattca caaatcagcg ccgttttatg accgtaaacg tccgccagat
     3721 cgtcccacat ttgacgtaga tgttgtccgc caatgatatc cattgcacct ctatccattt
     3781 ttgttcgttt gttattgggc gggcgctagt caggcaagcc gactgacgcc acgcgtttag
     3841 tcctcaactt tggccagac
//
LOCUS       DAJVQG010000231         3797 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJVQG010000231 DAJVQG010000000
VERSION     DAJVQG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.237"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(98..907)
                     /gene="proC"
                     /locus_tag="OQM83_003848"
     CDS             complement(98..907)
                     /gene="proC"
                     /locus_tag="OQM83_003848"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCS1521970.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1027..1485
                     /locus_tag="OQM83_003849"
     CDS             1027..1485
                     /locus_tag="OQM83_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCS1521971.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1668..2192
                     /gene="aroL"
                     /locus_tag="OQM83_003850"
     CDS             1668..2192
                     /gene="aroL"
                     /locus_tag="OQM83_003850"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCS1521972.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2242..2433
                     /gene="yaiA"
                     /locus_tag="OQM83_003851"
     CDS             2242..2433
                     /gene="yaiA"
                     /locus_tag="OQM83_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCS1521973.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2691..3368
                     /locus_tag="OQM83_003852"
     CDS             2691..3368
                     /locus_tag="OQM83_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCS1521974.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            3440..3724
                     /gene="ppnP"
                     /locus_tag="OQM83_003853"
     CDS             3440..3724
                     /gene="ppnP"
                     /locus_tag="OQM83_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCS1521975.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          951 a          904 c          990 g          952 t
ORIGIN      
        1 cgtgagtggt tgaaatcggc agatttggcg ctttacaaag caaagaaagc cggacgtaac
       61 cgcaccgaag tggcggcctg acgtccggcg aaagtcatca ggatttgctg agtttttctg
      121 atttttccat acacttcgtc atcgcttcga tcactgcggc acggaagcct ttctcttcca
      181 gtacgcgtac cgcttcaatg gtggtgcctc ccggtgagca gaccatatct ttcagtgccc
      241 ccggatgttc tcccgtttcc agcaccattt ttgcggaacc cattaccgcc tgagcggcaa
      301 atttatacgc ctgggcgcgt ggcatcccgc ccagcacggc ggcgtcggcc atcgcttcga
      361 taaacataaa tacgtaggct ggcgaagaac cgctcacacc gaccaccggg tggatcatcg
      421 gctcagcaat tacttccgct tcgccaaagc agcggaaaat attcagcaca tcagcggtat
      481 cttcgggggt taccagcgcg tttggcgtta cggaggtcat cccggcatta accagcgcgg
      541 gagtgttcgg catggcgcgg ataattttcc ggtcatggcc cagcgcgcgg gcaagctggt
      601 cgagcgtgac acctgcagca atagaaacga ccagagagtc tttattcagg ctggaggtga
      661 tttcgctaag cactttaatc atgatgccag gtttaacggc ggcaaaaatg atgtcggcga
      721 tttgcgccac ttcttgcgcc gattctgcgg cgttgatgcc gaactggtca tgcagggcgg
      781 cgactttatc cggggagggg gtgtataccc agatttgccc tggaagcacc tgaccgctgg
      841 caatcagacc gccgagaatg gcttttccca tattgccgca gccaataaaa ccgattttct
      901 tttccattgc ctcactcctg ccgtgaaatt cattgttttg ataatcgctg gcagaagcat
      961 aaacagaact atgccggaag gcaaaagcgc gacacaatag aggattaccc aacaaaggat
     1021 gactttatga caatttgggt ggatgccgac gcgtgtccca atgtaattaa agagattttg
     1081 tatcgcgcgg cggaacgtat gcagatgccg ctggtactgg tagcaaacca gagtttacgc
     1141 gtgccgccat cgcgatttat tcgtacgctg cgcgtcgcgg caggtttcga cgttgccgat
     1201 aacgaaattg tccggcagtg tgaagcgggc gatttggtga tcaccgcaga tatacctttg
     1261 gctgctgaag ccatcgagaa aggcgcagcg gcgcttaatc cgcgcggcga acgttacacg
     1321 ccagcgacca ttcgtgagcg cctgacgatg cgcgatttta tggatacctt acgtgccagt
     1381 gggatccaga ccggcggacc agatagcctt tcacaacgtg accgccaggc ctttgccgcg
     1441 gagctggaga agtggtggct ggaagtgcaa cgtagtcgtg gctaaatgta atttattatt
     1501 tacacttcat tcttgaatat ttattggtat agtaagggat gtattgagat tttcacttta
     1561 agtggaattt tttctttaca atcgaaattg tactagtttg atggtatgat cgctattctc
     1621 atgacaccgg ctttcgccgc attgcgacct attggggaaa acccacgatg acacaacctc
     1681 tttttctgat cgggcctcgg ggctgtggta aaacaacggt cggaatggcc cttgccgatt
     1741 cgcttaaccg tcggtttgtc gataccgatc agtggttgca atcacagctc aatatgacgg
     1801 tcgcggagat cgtcgaaagg gaagagtggg cgggatttcg cgccagagaa acggcggcgc
     1861 tggaagcggt aactgcgcca tccaccgtta tcgctacagg cggcggcatt attctgacgg
     1921 aatttaatcg tcacttcatg caaaataacg ggatcgtggt ttatttgtgt gcgccagtat
     1981 cagtcctggt taaccgactg caagctgcac cggaagaaga tttacggcca accttaacgg
     2041 gaaaaccgct gagcgaagaa gttcaggaag tgctggaaga acgcgatgcg ctatatcgcg
     2101 aagttgcgca tattatcatc gacgcaacaa acgaacccag ccaggtgatt tctgaaattc
     2161 gtagcgccct ggcacagacg atcaattgtt gattttagag cgcctatact taacgttcat
     2221 cccgtgaaat aaggaagaac gatgccaacg aaaccgcctt atcctcgtga agcatatata
     2281 gtgacgattg aaaaaggaaa gccaggacag acggtaacct ggtaccaact cagagccgat
     2341 catcctaaac cagactcgtt gatcagtgaa catccgacgg ctcaggaagc gatggatgcg
     2401 aaaaaacgct atgaggaccc tgacaaagag tgaccgcatc agactgctcg gaagggattc
     2461 tgagtgccac tacaagggat ctgcgtcaca tttttcataa ttcatgtttt tctaataatt
     2521 agaatattaa acaataacaa tccattactg gaatcatttg gaatctttac attatgccgt
     2581 gcacgtctgc tgctacgctt tttgtcattt gtagcacaag taagtgtcag cagtggtgct
     2641 ccacacttgc ccggtaatta acgacgaaag aaaagtaagg tggatgaaca atgagtgcgt
     2701 cgttggcgat cctgaccatc ggcattgtac ctatgcagga ggttttgccg ctcctgacgg
     2761 aatacattga cgaagataat atttcccatc atagcctgct ggggaagtta agtcgtgaag
     2821 aagtgatggc ggagtacgcg ccagaagcag gcgaagacac cattctcaca ttattaaatg
     2881 acaaccagct ggcccatgtt tcgcgtcgta aagtggagcg agacctgcaa ggtgtggttg
     2941 aagtgctcga taatcagggt tatgacgtca ttttattaat gagtacagca aacattagta
     3001 gtatgactgc gcgtaatacg atctttcttg agccgtcgcg aatattgcct ccactggttt
     3061 cctctattgt tgaagatcat caggtggggg ttatcgttcc ggttgaggag atgctgaccg
     3121 ttcaggcgca aaaatggcaa attttgcaga aatcgccggt attttcattg ggtaacccca
     3181 ttcatgattc agaacaaaaa atcattgatg ccgggaaaga attactggca aaaggggctg
     3241 atgtcatcat gctggattgt ttaggatttc accaacgtca tcgcgattta ctgcaaaaac
     3301 agctcgatgt tcctgtcttg ctgtctaacg tattgattgc acggctggct gcggaattac
     3361 tggtgtaatt ttgcgtgaca gccagcgcct ctggccccta tagtgaagta gatgttcaac
     3421 taccagacag ggccagttta tgcttcaaag taatgagtac ttttccggca aagtgaaatc
     3481 aatcggcttt tccagcagca gcactggtcg cgccagcgtg ggtgttatgg ttgaaggcga
     3541 atacaccttc agcaccgctg agccggaaga gatgacggta atcagtggcg cgctgaatgt
     3601 gttactgcct gacgcgaccg actggcaggt gtatgaagcc ggttcggtgt ttaatgttcc
     3661 cggtcacagt gagtttcatc tgcaagttgc cgaacccacc tcttatctgt gccgctatct
     3721 gtaattcctc gccttcccct tgaacgggag ggcatttttc tgaaatatcc tctctttaac
     3781 ccataataat atttcct
//
LOCUS       DAJVQG010000232         3792 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJVQG010000232 DAJVQG010000000
VERSION     DAJVQG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.238"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..858)
                     /locus_tag="OQM83_003854"
     CDS             complement(<1..858)
                     /locus_tag="OQM83_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCS1521976.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQD"
     gene            complement(855..2252)
                     /locus_tag="OQM83_003855"
     CDS             complement(855..2252)
                     /locus_tag="OQM83_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCS1521977.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2407..3405
                     /locus_tag="OQM83_003856"
     CDS             2407..3405
                     /locus_tag="OQM83_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1521978.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     ELIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3516..>3792)
                     /gene="ompG"
                     /locus_tag="OQM83_003857"
     CDS             complement(3516..>3792)
                     /gene="ompG"
                     /locus_tag="OQM83_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCS1521979.1"
                     /translation="TQEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDF
                     NRVGLFYGYDFQNGLSVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT          927 a         1039 c          936 g          890 t
ORIGIN      
        1 atcttgcgac atccagtcca tccccacttc gcgcaccagt tcgctggctc cggcaaaagc
       61 gatattcagc aataccagct taaacagacg caaacggctg taatacccca gttcaatgcc
      121 atacagcgtg gcgatgcgat taattaaacg cagattgcgc caggcgataa acgccatatc
      181 gaccaacgcc agcgggctga ccgcaatcat caacgttgat tccgccgccg aacggctgat
      241 ttcgcgccgc gcctgggcat ctaaaactgg ctggaccaaa tgcgcataca ggctgaccac
      301 ttcacggtcg ttttgcgttt catggattga ggcataccag cgttgcagcg ccggatgcga
      361 ctgatcaata cccgcctgct gcgccagttt ttcgcaaaat gcgcggcctt tgcccgtgcc
      421 gtggctatgc aataaatcac gcgcttcgtc gcgttcatgg gcgcgctgtc gcaagcacca
      481 taagcactgc cactctgtta ccacagaacc tacgccagcg ccgataatca atgccccagc
      541 ggcacatcca cccagcgcta cccagtcctg ggtttgccag gcattcattg tccactgtac
      601 accctgcccg acaacacttg cgccaaacag agccagcccg cccatcacca ttttgcgcca
      661 caggctgcgt ttcggacgta acgctgcatc cattaccgct tcaacttgcc cctcttcttc
      721 aggcgcttca tcgagcgtgg ccggggcaaa attttgcgcc tgattttcgt caaaggtctg
      781 ctgcgccctg aattttggat tctgatcgac ctccagcgga ccgtcgaaat caatacgtgg
      841 ttttaacggt tcggtcatcg caatttatct cctattaaaa attccagcgc ggcatcaaga
      901 cgaatatgcg gcagcggttt gtcgacatcc atcacctgcg ggcgaaacgc ttcaaactgg
      961 aacccttgct tgtcccagaa cgcctgaccg ggcaaacgcg caggaacttc accggggtaa
     1021 acggtgagag gcgcaccatc gctgagccga ttaccgcgca atgccggaat tttctcgccg
     1081 ttgacatcaa taatgccgct ggtggtcgcc tgcaccgagg ccagccccag gcaatccata
     1141 ctaatccctt caaacgccgc attttgccag gcatcctgaa tcagttgttg cagtaatgaa
     1201 accatattgg cgtgctgatc gaccgttaca tggtccgctt tcgttgcggc aaacagcagt
     1261 ttgtcgataa ccggcgagaa taagcggcga aagagcgtgc gctgcccgta gtggaaactt
     1321 tgcatcagct gcgtcagtgc cagacgcata tcattaaatg cctgtggccc actgttgaga
     1381 ggttgcaggc aatccaccag cacaatctgg cggtcaaagc gcagaaaatg attcttatag
     1441 aaccccttca ccaccttctc gcagtaataa ttaaaccgct cgcgcagcat tccggcattg
     1501 gtgtgcttat cggcctgcgc cagtttggac tcgccccagg tatcgacatc cggccacggg
     1561 aagaattgca gcgcgggcgc acctgccata tctcctggca agacaaagcg ccctggctga
     1621 ataaagtgca gcccctgctg tttacagtgg tggagataat cggtccacgc ggcggcaatg
     1681 tctgccagcc ggttttcgtc ggcaggtgct agcgggtcca gcccttcgca catcattcgc
     1741 catttgaccg accattcgcc gcgctgacca ttgagtaagc ccgtcatctg gcgtgaccag
     1801 cttaaatagt cctgcgccag catcggcagg tcgagcaacc attcgccggg ataatccaca
     1861 atctccagat acagcgtgga ggtgtctttg aagtggcgta gcagcgaatc gttcgattta
     1921 aaacgtagcg ccaggcggat ttcactgacg ccgcgcgtag gcgtcggcca ggcgggagga
     1981 tcgccatata actgcgccag tccttcatca taggtgaagc gcggaatgcc aaagtcacgc
     2041 tgaggaatgc gttttacgcc cagcaggcgc tcttcacgca ccgcgcttaa cagcggcaag
     2101 cgtgctccgg cgtgaatatt gagcaactga ttgaccatcg cagtgataaa cgctgttttg
     2161 ccgctgcggc ttagtccggt tacggcgagg cgcagatgtc tgtcgacacc ccgattcacc
     2221 agcgcattaa gttcattttt aagtcgcttc attgctgtcc tgtgctgctc tgaagaccag
     2281 aaaaaaatca ttcgtaagga tatgaatgtg ggggccaggc cattgattat caactgcccg
     2341 tgttgtcatc gacgggcgaa ctccataaga tgtgcagcat tcacgttcgg acggagttcc
     2401 tgacctatgt cccctactat ttatgatatt gccagggttg caggcgtatc aaaatccacc
     2461 gtatcacgcg tgctgaataa gcaaactaat atctccccgg aagcgcgcga aaaagtgtta
     2521 cgggccattg aagaattaca gtatcagcca aacaagctag cccgcgcgct gacctcttcg
     2581 ggttttgatg ccattatggt gatttctacc cgttcgacca aaactacggc gggtaatccg
     2641 tttttctctg aagttttgca tgccatcacc gccaaagctg aagaagaagg tttcgacgtg
     2701 atattgcaga cgtcgcacaa cccggcagaa gacttacaaa aatgtgaaag caaaattaag
     2761 cagaaaatga ttaaaggcat tattatgcta agttcgccag cggatgagtc attttttgcc
     2821 caactcgata aatacgatat tccggtggtg gtgattggca aagttgaagg tcaatatgcc
     2881 catgtttatt ctgtcgatac cgataatttt ggcgacagca ttgcgttgac cgatgcatta
     2941 attgaaagtg ggcatcaaaa tattgcctgc ctgcatgctc cgcttgatgt ccatgtttca
     3001 gtggatcggg taaatggtta taagcagagc ctggctgcgc ataatattgc agtgcgtgat
     3061 gaattgattg ttgacggtgg ttatacccat gaaacagcac tgcaagccgc gagagaatta
     3121 ttaagccagt caccgttgcc tgaggcggtg tttgccactg acagtctgaa attaatgagt
     3181 atttatcgtg cggcagcaga gaaaaatatt gctattccgc agcagttagc ggtggtgggt
     3241 tatagtaatg aaacgctgtc gtttatatta acgcctgcac cgggcggcat cgatgttccg
     3301 acgcaggagt taggtcaaca aagctgcgag ttattattcc gcttaatttc tggaaaaccg
     3361 tcaccacaga atattaccgt tgccacgcat atgacgctga aataaatacc agctaccttg
     3421 cctgaatgcc ggatgcgatg ctagcgcatc ttatccggcc tacatttata gatcggataa
     3481 agtactcgcg ccgcatccgg caatttagcc cattatcaga acgagtaatt tacgccgaca
     3541 cctgcataat ggaatttatc actgtctcct tcgtcgtgat cctgccactc aaacgcgtat
     3601 tccagcgaaa cggaaagtcc gttctggaaa tcataaccgt aaaataaacc tacacggtta
     3661 aaatcatggc cttcacgttc aatatcatcc tgccagtccc agttactcca gcgatccagc
     3721 ccaatgcgcg tatacggcgt caccgagtgg ttgccgagcg tcagcggcaa ataggcgcga
     3781 atttcttgcg tg
//
LOCUS       DAJVQG010000233         3730 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJVQG010000233 DAJVQG010000000
VERSION     DAJVQG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.239"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            65..172
                     /locus_tag="OQM83_003858"
     CDS             65..172
                     /locus_tag="OQM83_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCS1521980.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(259..1938)
                     /gene="bcsG"
                     /locus_tag="OQM83_003859"
     CDS             complement(259..1938)
                     /gene="bcsG"
                     /locus_tag="OQM83_003859"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCS1521981.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1935..2126)
                     /gene="bcsF"
                     /locus_tag="OQM83_003860"
     CDS             complement(1935..2126)
                     /gene="bcsF"
                     /locus_tag="OQM83_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCS1521982.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHFLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2123..3694)
                     /gene="bcsE"
                     /locus_tag="OQM83_003861"
     CDS             complement(2123..3694)
                     /gene="bcsE"
                     /locus_tag="OQM83_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCS1521983.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT          956 a          937 c          950 g          887 t
ORIGIN      
        1 aatcttgaac ccaaacaact caagaatagc cgcgaaacgt tggcattaca acacaggcgg
       61 ctatatgacg ctcgcagaac tgggcatggc cttctggcat gatttagcgg ctccggtcat
      121 tgctggcatt cttgccagta tgatcgtgaa ctggctgaat aagcggaagt aacgtgtcat
      181 gcgggcgtca ggctgccgta atggcaattt gcgcccggat caggccgcag aggttaaact
      241 ctgcggcctt tttcgtgctt actgcgggta aggcacccag tcgccgccgt tcaggcgaac
      301 gtacggttta tcctggtatt gaataactac tgcatttgag ttctcggaga ccggtgctgt
      361 ttgtggcaac ccactggtga gttttttcca gtcaacattg tcttcggtga aaatcttgcc
      421 atcaagaacg cgaaccacca gatcggagat agctaggaag ctgctcggtt ggtcaatgac
      481 aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat ttcaccccaa cggggacgtc
      541 tgtgatagac gggctaggga tatcacgtag gccagatacc tgcattctgt cgcccttcag
      601 cgcgccgccg tgttccggca ccacgaccac catcacttta cgacccgatt tctccagttc
      661 agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc cgcgctttgt aatccgctgt
      721 tttgctgacc cccggataat ggttgccgtc atgcagtgga agcgtgttgt agaacgtggc
      781 actacggctg tttttatctt tttcggtaac gtccagccag cggttaagca cggcggtatc
      841 gtcataaacc ggcgaaccat caaagcccag caaaataacc ggcagatttg tttgatccat
      901 caattcagtc tgcatgccgc cattttcgcg aacttctttc aaaaaaccgc cgaactggcc
      961 gttatgtccc atcatcaggt gctgggtaaa gcccagtttc gaaaggttat caaacagata
     1021 gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa gtctgcccgc agctggcgcg
     1081 cagtaaacgg atcgccgccg ggccactgta ggaggtggcg gagttaaagt tcttgaactc
     1141 aatatcgaaa tgcgaccaca gtggatgcga catcaacccg gcggcttcta tatccgacca
     1201 ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc tgagcatcag cgggcagcga
     1261 agacgggaag gtcgatttac gtttcgcctc cgcgttatag aaattattca gccaggcgtt
     1321 aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata tcacccacta ccgatgcgcc
     1381 acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc gttgttacag tggtcgtcgg
     1441 ttgtccggct ggccacaagg agaaacttgg tcccgccagg gtaagtacgt tcagccatag
     1501 cagtatggca accacaaaaa cggtaatgcg aatccattgt gacaggaata accaggccac
     1561 taataaaaca aaaatggccc caatcatctg ccagttaata aagcgtgtga caaggtcgat
     1621 taaataatcg gtactgaacc ccgccacctg cgaaccctgg ctcattatgc tttccgggcc
     1681 aggcaaccag gtgtcatgcc agaacaaagc aaagccgatc ggcagggcaa tccagtggcg
     1741 caagcgatgc aggctgtagc gcggaatggg catcagcaga aacgtggcaa acaccaaatt
     1801 gaggagcgga tggaagttaa gatatcccgc ccacaacagg ccgaacttaa ccagaaaata
     1861 gaagttccag ccggaaaggc cgcgccagta ttgccagagg gaagaaggca tggcggtatt
     1921 ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt ccgtgcggcg taacgtcccg
     1981 gccggtttaa cataacgagg tttagcaaag aataaacgta aggtgtcgcg aatgcgtcgc
     2041 aaaaagtgcc gcgccagata gcccagcggg aaaaatatca gtgcgcaaac gacaataatt
     2101 tcaatgatat cgctgatggt catcatgatg agcgctccac agcatcatct aacagtcgca
     2161 tgggttctgg tattcgtcgc cagtggcgac catcgtgctc ggcattgatg accggtttag
     2221 aactttgcgt taaaggcagc ggcatgcccc attgttctgg ggcaagcaag cgcatctgca
     2281 ccagctcggc actgatttga tcatcttcaa accagaccat acggtttgag aaaatgtcgc
     2341 cagtaggcaa tgggaaaata tgattcaacg cggtatccag atcgttaatc cgacagaatg
     2401 agagaaacag caccagccga ttaccgccaa tggtcatgat atcgccggta cggttagggc
     2461 gacacagcgt cagggcttgt tcaacgcgga tacccggtac cggacgtagg gcaaccagaa
     2521 cgcctttacc gtgggcaggt aatagagggt tattcatcat gttgttgacg gcattacaga
     2581 acacatccca cttctggaaa ccacgcagtt tgagcggctg ggtcattgac agcaaggtag
     2641 tgatatcttc cggcacatag cgactaaact tctgcccttg cacgctttcg atcatcgtca
     2701 gacaacggga gagtggcgca ttccacggaa taaccatatt tgcaccgcag gccaataaca
     2761 aacgttcatc ggtggcgcgc aggctagcgg tattttcccg cacgaggatt ttcatcgcac
     2821 taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag tggctcgatt tgcgcatttt
     2881 gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg ggcttcattg aacaggactt
     2941 cgttattgtt gaacagttgc cagtgttccg atagcggcgg cgcaccttcc agtacagcaa
     3001 cattactcag aatgcgtttt tcgtcgctgc gtggttggat ctccgcctct tcgctttgaa
     3061 ctaatgtcca gataccattt tgttgctgaa cgctaagctg ctgacgggcg ctgacccctt
     3121 tttcgttgca ccagaaggca atatccagca aatgttggtc accctgaaaa cgcaaactgg
     3181 caagaccaaa aagtgaacgg tattcctcaa gcaacaatga aaattgttta tcgttattat
     3241 ttccgggatt aattaccaaa agcgaacaat ggtttaacct gctccattta ttcattttat
     3301 ccaaccatga gcgaagccgc tcggcaggaa tgttttgcca tgcgttattt gcgcaaacaa
     3361 gaataaaaag gtaattatgg ggatcaatag aacactgcaa atcacgggcc aaatagtata
     3421 gacctttttc atgatttagc attgaaaata attttatttt ttccggccct tgagaatcat
     3481 ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat gaccgcgacg tgtgcggttt
     3541 cagcctggga tgcaattgtt tgattcgcca gactgatagc atcttcatgg cgatcgacgt
     3601 taaaccacca gacgccgcct gctggcatat gtcgcagctc atcccataat gatgagatac
     3661 cgatagagaa tacagggtcc acaatgtccc tcatgtcacc attttgtctg attatcagtt
     3721 tactagcgaa
//
LOCUS       DAJVQG010000234         3645 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJVQG010000234 DAJVQG010000000
VERSION     DAJVQG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.240"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            418..1307
                     /locus_tag="OQM83_003862"
                     /pseudo
     CDS             418..1307
                     /locus_tag="OQM83_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049841.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1307..1597
                     /locus_tag="OQM83_003863"
     CDS             1307..1597
                     /locus_tag="OQM83_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521984.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            1918..2208
                     /locus_tag="OQM83_003864"
     CDS             1918..2208
                     /locus_tag="OQM83_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521985.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHIALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            2208..2627
                     /locus_tag="OQM83_003865"
     CDS             2208..2627
                     /locus_tag="OQM83_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521986.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYIKPLINKDN"
     gene            2795..3118
                     /locus_tag="OQM83_003866"
     CDS             2795..3118
                     /locus_tag="OQM83_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521987.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNVENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
BASE COUNT         1204 a          650 c          743 g         1048 t
ORIGIN      
        1 gctctcagaa tgcctctgag cacgtcatac aggattgctc aaaggttgag ctaaaattct
       61 cataaatatg gtcatttaca agacaggtga ttcagatgtt aagaagtatc cctgtgaaca
      121 ataaatgacc aatcagtaat cattctgtga aataattgtt gcaatttata atcgcatctg
      181 ttatagtttc tatatgggct gataaaaaag ttcatgtctt gaagtggtaa cacttgggga
      241 ggtgtcagat ggttggcgta tattaacctc tcattcaggg tatacctcag cgttttaatc
      301 atcgtactgt ttcaggcatg gacttcttta tcagtccatc aatcccccag atattttaat
      361 cacacaggct aatcggtttt atccggttgg ccttttcacg tatgaggtaa tttatgaatg
      421 tctaaatata acccaaatca gttttataaa tttgatgtgc gaatctctca actttcagaa
      481 gaaattaacg cagcagcaag attgatttat atggtcatgg tccatagctg tgacttctat
      541 gaagagaaat acaaatcata ttcaccaagt attgaccatc tcgcttatat ggctggtgta
      601 tcaaagagaa cagcaatcag agccattgag acacttgaga aacacggttt aattcaggtg
      661 caaaagaatc aggggcgtca aagcacttat cgtgtattca cactggatga taagccggaa
      721 ttacttgtaa gacctacatc acaggaagtt gcagcctcag taagacaaaa tgacaccact
      781 accagtgaca aaatgtcatg ggggtagtga caaaatgtca cccgtgttag agtttagata
      841 agttattaga taaaacatta gataataata ttacagataa gaataaagag actggactta
      901 atgatgatat taaagaagat gactttcttt ctttggatga attaaatgat tttctctcta
      961 tgaatgaatc tgaccttgaa gtaagctcta aaaagacttc taaacaagac agagaaatca
     1021 ctgatgtgag tgttgatagt gaccataaaa acacacagtg ccccgccact gatgatgagt
     1081 tggacccctt cgatgatgaa gaggatgaac gtgaaaaagc cattaagagg gcagagaaga
     1141 aaaagagatt cgagcttgca agacaaaaga acaaagtatc gggtttagac ccattcgatg
     1201 acagtgaaga tgattattat ctttcaccga aaaagaaaca aactcaggat gacaagaagc
     1261 aaaaaattaa accgaagatg tattttgccg gtggaggaat taagtaatga aagaatatca
     1321 cggtgaaaag agatacaaag attacctgtt aaagcgttac agcatttctc gtgaaggtca
     1381 tcttatgaag gatactcacg gggaagttta ccggattaga ccaaagaaag aagggagggg
     1441 ttatttcttt tacgatggtg tgacagattt aaagattgac gctttgaggt ttgcagtgct
     1501 gtatcatttt gatatctgga attcaatcca ccagctacga ttaaaagacg gtgaccccag
     1561 caacttaaag gccaccaata ttatcacaaa acgttagttt gttttaccat catcaatcgt
     1621 aattgtgatt tcatatttga aaagcttaat gctgaaattg aatcgtgtac ttctggcgat
     1681 aatcattttt aaaagctgat acacaagaag caccagaaac acaaagcaac ctaagcttgc
     1741 gaatctgtga gattcagcaa ttgtttcaag aaagcccatt aattcccctt atttatcaaa
     1801 gggaatgacc tgtatcagcc ccctacaacc atattaacat gtaaatgagt tgttgacaat
     1861 atgttataaa tatgttaaag ttagttataa gtcaaagatt ttaagaagga aaatttaatg
     1921 agacacacag ttacacgcct tttgccagag gcatattcca gactggcagc aatcaaagac
     1981 aaatacccag ccatgagcca cacagcactt gttggtgaga ttttgaagga acacatagcc
     2041 cttacaccgt ttgacgctat caagggcgtt gcagagggtt ctaaggccat cagaatcaca
     2101 acgggccaac tggaactact caaagcacag tccgcagtgg caggttgctc aatgattcag
     2161 gtgttgagtg ccaaggttca tcaactggcg gataagctgg gggtttaatg ctatgcacat
     2221 ttgattatca tgcgttttat cgccatagag gtcaccttgt ccgtgagaat aacggaacat
     2281 ggaaagattt aactcgtaat gctgaaccct tcacacacca gcaattaaaa tcccgcttct
     2341 ccagatggaa gaatgcacca gcagatactg gtgattttga gattatccat accttcgatt
     2401 atgcctcaga agatgacctt cacgatgcaa ttaagctgat tgaagacttc aatacctcag
     2461 aagatgtgga tggtgacaaa tataaatccc gtggtaaccg ctataaccgt gatgatatca
     2521 ctcaggccat aaatgctggt caatctcagc gatacattac agaaaaatat ggtatttcac
     2581 ggagacagta tttctacatt aagccattga ttaataaaga caattaaaaa taaccatcta
     2641 agaaacggta gaatgcactt tacaggcaga gttgcacctt aatttgataa attaccttac
     2701 cagccagtgc aactccctct gtaacccgca tggttactgg cctcagaatg gctattttaa
     2761 gaggtctgag gtgtgtttca taggagattt aaaaatgaat aattacccaa tgcaaattat
     2821 ggttgataac gacactgcta tgatggtgca aagcttcatt gatgcaggta tcgcaattaa
     2881 ttttgataaa ttactaaagc tgatggctga taatgtagaa aacatctctg actttattca
     2941 gtcggtggag tttaacgagc ctcgaatgat gctaccaatt acagacagca acatgaaaag
     3001 attggttatc gagcaaacga acatatacag tatttcccca gagcaatttt tgaaaggtgc
     3061 tgtgacaatc ctgtacagtg ataatattct ggtgacggat tctgtgaggg ttcactaatg
     3121 ggtttaataa ttgcttggac gttgcttggc ttatttgttg gttgtattat taacaatgcg
     3181 gtaaataaga attgatgtta tcaacctgtt aaaaagctgt tgacaaataa ccttaagcat
     3241 ggcataattt taattatggg agagcaattc tctcttattt ttctttcaat aatccgatgg
     3301 atggatattc tgagttggaa aggtgcaatg cttttcgtca tggcctttca ggttagtctg
     3361 agaggctatc tcaaaaagta ctcttgttca ttcattattg aatcctttgc ccttcacaga
     3421 aatgtttagg gctttttcag tttttaaaag cacacttggc aggttcttgt tcagtcagac
     3481 tcttcacctc tgattgctga gtgtgcttct ataaattgaa ataacaaata aactaagggg
     3541 tgaacgaatg attagaaatg aacatgaaat gcttatcgaa cacagaccaa gtgcacttga
     3601 tattcacgaa ttaactttgc gtgaatcttt tgatagagct tggcg
//
LOCUS       DAJVQG010000235         3637 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJVQG010000235 DAJVQG010000000
VERSION     DAJVQG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.241"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(80..397)
                     /gene="hspQ"
                     /locus_tag="OQM83_003867"
     CDS             complement(80..397)
                     /gene="hspQ"
                     /locus_tag="OQM83_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCS1521988.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(455..1645)
                     /gene="rlmI"
                     /locus_tag="OQM83_003868"
                     /pseudo
     CDS             complement(455..1645)
                     /gene="rlmI"
                     /locus_tag="OQM83_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            1740..2018
                     /gene="yccX"
                     /locus_tag="OQM83_003869"
     CDS             1740..2018
                     /gene="yccX"
                     /locus_tag="OQM83_003869"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCS1521989.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(2015..2344)
                     /gene="tusE"
                     /locus_tag="OQM83_003870"
     CDS             complement(2015..2344)
                     /gene="tusE"
                     /locus_tag="OQM83_003870"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCS1521990.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(2435..3094)
                     /gene="yccA"
                     /locus_tag="OQM83_003871"
     CDS             complement(2435..3094)
                     /gene="yccA"
                     /locus_tag="OQM83_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCS1521991.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(3301..3388)
                     /locus_tag="OQM83_003872"
     tRNA            complement(3301..3388)
                     /locus_tag="OQM83_003872"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3352..3354),aa:Ser,seq:tga)
BASE COUNT          982 a          934 c          876 g          845 t
ORIGIN      
        1 ctattgagat ccctcgcctg ggcctggcca aataaaaaat ccccggaagg caaaaacctt
       61 ccggggattc gttcagggat cagttacgca gacgcggggc ctggagttgt ttgcggatgg
      121 tctgcgccag ctcatccatg gatggctgtt caggatgttc atcctgcaat tcgctactta
      181 gctgggcttc agccaggtag gtatgaaccg gtaggccgtt atcgtcctcc atcactacgt
      241 gataccacgg agcggcgcga agctcgtcat ttaccgccag ctcatcaggc gacggttcag
      301 agagcgaata aaccgggtcg atatccacga ccactccgag ataacctaac agggaatggc
      361 ggacctgctg gccgataccg aatttgctgg caatcatagt cacctcccgg gaaatcttta
      421 cctttgatat aagggtaaaa ttccacattt caagttacat gacgcgacag gcaaaccctt
      481 tcagatatag cccttccgga taggtagcga tcaccggatg atcggctgcc tgacggaact
      541 gctctataaa ttgtacatca cggccggcat caattgcggc atccgcgatg attttctgaa
      601 ataaatcgct ggtcatcaga ccggaacagg agaaagtcag gagaataccg ccttcattca
      661 gcaactgaat cgccagcatg ttgatatctt tatagccacg acacgcgccc atcaactggc
      721 ttttattctc aacaaacttc ggcgggtcca tcacgataac gtcaaatttt tcaccgcgat
      781 cgcgataagt acgcagcaat ttaaagacat catcacggac aaactcagcc ttgctcagat
      841 ccagtttgtt cagctcaacg ttctgccgtg caatatccag cgcttcctag gaggtatcaa
      901 cgctgacaac ctggctgcaa ccgcccatca gtgccgatac ggcgaaacca ccggtatagg
      961 agaaacagtt cagcacacgt ttattttcaa cgtagcggcg ggtagccagg cggctgtctc
     1021 gctggtccag gtagtagccc gttttgtgtc cgtgctgaat atccaccagc agcttcatgc
     1081 cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc ggtgacgagg ccctgggtca
     1141 gctccatccc ttctttttta cgtaccgcaa catcgctgcg atcgtaaatc gcacattccg
     1201 ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg atattctgcc ccagcactca
     1261 gcagttgcag caccagaaaa ttaccgaaac gatcgatagt aatacccggc aggccatcag
     1321 attctccggc gattaaacga tagctgtcga ggccatcttt ttgcgccagt cagtcacgcc
     1381 atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat gtcgatagac tcagacgggt
     1441 caaacgtcca gacgcgcgcc cggatttgcg aagctggcga ataagcgccg cgtgctaacc
     1501 attttccctg atgatcaaca atatcgatgg tttcaccgag gctggcttta ccttccatgc
     1561 gggcaacggc cccggaaaag acccacggat ggcgacgaag taatgatttt tcgcgccctt
     1621 tggctaacac taaacgtaca ctcataattt acttttctgt cgatgcaaag aaatgggcgt
     1681 cattgtccgg agttttccat ggatttgcaa cgcgtccatt aaggataagg agagcagcaa
     1741 tgtcgaaagt ctgcataatt gcctgggttt acgggcgagt tcagggcgta ggatttcgct
     1801 acaccacaca gtacgaagcg aaaagactgg gtttaaccgg gtacgccaaa aatcttgacg
     1861 atggcagcgt ggaagtggtt gcctgcggtg aagaagggca ggtggaaaaa ttaatgcagt
     1921 ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg ggtgctcagc gagccgcatc
     1981 atccctcggg ggaattaacc gattttcgta tccgttaaat acattttacc ggttttggca
     2041 ggccagcaat tttggtggct tgcttagccg gacctttcgg aaacagtcgg tataaatagc
     2101 ggctgttacc tttttcttcg ccaaatttat tcgccatcgc ttttaccagc atacgaatcg
     2161 ccggagaagt attgaattcc agatagaaat cacgcacaaa acgcaccact tcccagtgtt
     2221 ccggtgacag cgaaatccct tcgttctctg caatcaccac tgccagcggc tcactccact
     2281 ggctgctttc tttgagatag ccttcggtat ccgtttctat ctctttacct tcgaagatca
     2341 gcataattat tactacctta atcagactgg cggcagtgta acaaaaaaca aagccccgca
     2401 taaagcgagg ctatgaaagt gttagcgggt gagattaatc gcggctggcg aagcccagaa
     2461 tgctcagcag gctgacgaag atgttgtaca gcgaaacata caggctaacc gtggcacgaa
     2521 tatagttcgt ttcaccgcca tgaacgatgt tgctggtttc aaacaaaata gcgccagagg
     2581 agatcagaat gaagaccgcg ctgatcgcca gatgcagagc aggcagctgc aggaagatat
     2641 tagcaaccat accaatcagc accaccacaa tacccgccat cagcataccg ccgaggaacg
     2701 acatatcttt gcgggtggtc agcacatatg cagagcagca gaagaacact aacgccgttc
     2761 cgcccagtgc catagcgatt acgtcaccca ttccggcaga cagataggtg ttcagaatag
     2821 gtccgaggat ataacccaga aaaccggtaa aggcgaatgc ggagataatc ccggtcggct
     2881 tattcgccgt tttataggtc aggaacatca aaccatacat acccaccagc gtcagaatca
     2941 gacccggaga tggcagcatc agcaccgtac tggcagttgc ggtaatcgcc gaaaaggcca
     3001 gcgtcaggct cagcagaaaa taggtattac gcagcacctt atgggtgcta agcagtgatg
     3061 tacggtcatg tgaagaacta acaatacgat ccattgagtc actctctatg acagatgtaa
     3121 ttaattaagc agcataatga taatgcgtaa gggcacccag aagttttacc catctttacg
     3181 catttgatct ggaacaggtt taacagcgga ttatcaggtc attaagcaaa tataacgccc
     3241 tgagaatttc gacaggcaaa agaaaaaggg gttagcattt agctaacccc ttatcttatt
     3301 tggcggaagc gcagagattc gaactctgga accctttcgg gtcgccggtt ttcaagaccg
     3361 gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc actataaaca tcccgatgcg
     3421 gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag ccaaaagtgc attgatagcg
     3481 tgaaataaca gcagattgat catttcatca ccatgaattc cttctctttt actcgtttag
     3541 caaccggcta aacatcccca ccgcccggca aaaagaaaaa taggtccatt tttatcgcta
     3601 aaagataaat ccacacagtt tgtattgttt tgtgcaa
//
LOCUS       DAJVQG010000236         3375 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJVQG010000236 DAJVQG010000000
VERSION     DAJVQG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.242"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            6..326
                     /locus_tag="OQM83_003873"
     CDS             6..326
                     /locus_tag="OQM83_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine transporter"
                     /protein_id="HCS1521992.1"
                     /translation="MTMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASY
                     RYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKD
                     SQRA"
     gene            428..1882
                     /gene="amn"
                     /locus_tag="OQM83_003874"
     CDS             428..1882
                     /gene="amn"
                     /locus_tag="OQM83_003874"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCS1521993.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2213..2929
                     /locus_tag="OQM83_003875"
     CDS             2213..2929
                     /locus_tag="OQM83_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1521994.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          933 a          738 c          737 g          967 t
ORIGIN      
        1 gagtcattac catggcgctt gaagcacaat ctattttctg gatagttttc ttctccataa
       61 tgctggcaaa cattgcgcat gacatggtgg tgtgtgtgca acaaccgatg tttaccgaaa
      121 tgtttggtgc cagttatcgc tatagtggcg ctggagtcgg ttatcaggtt gccagtgtgg
      181 ttggcggtgg atttacacct tttattgccg ctgcactcat cacttacttt gccgggaact
      241 ggcatagcgt cgccatttat ttgctggctg gatgcctgat ttccgcaatg accgctttgt
      301 tgatgaaaga cagtcaacgc gcttgatagc ctggcgaaga tcatccgatc ttcgccttac
      361 acttttgttt cacatttctg tgacatacta tcggatgtgc ggtaattgta tggaacagga
      421 gacacacatg aataataagg gctccggtct gaccccagct caggcactgg ataaactcga
      481 cgcgctgtat gagcaatctg tagtcgcatt acgcaacgcc attggcaact atattacaag
      541 tggcgaatta cctgatgaaa acgcccgcaa acaaggtctt tttgtctatc catcactgac
      601 cgtaacctgg gacggtagca caaccaatcc ccccaaaacg cgcgcatttg gtcgttttac
      661 tcacgcaggc agctacacca ccacgattac tcgccctact ctctttcgtt cgtatcttaa
      721 tgaacaactt acgttgctgt atcaggatta tggtgcgcat atctctgtgc aaccctcgca
      781 gcatgaaatc ccttatcctt atgtcatcga tggctctgaa ttgacacttg atcgctcaat
      841 gagcgctggg ttaactcgct acttcccgac aacagaactg gcgcaaattg gcgatgaaac
      901 tgcagacggc atttatcatc caacggaatt ctccccgcta tcgcattttg atgcgcgccg
      961 cgtcgatttt tccctcgcac ggttgcgcca ttataccggt acgccagttg aacattttca
     1021 gccgttcgtc ttgtttacca actacacacg ttatgtggat gaattcgttc gttggggatg
     1081 cagccagatc ctcgatcctg atagtcctta cattgccctt tcttgtgctg gcgggaactg
     1141 gatcaccgcc gaaaccgaag cgccagaaga agccatttcc gaccttgcat ggaaaaaaca
     1201 ccagatgcca gcatggaatt taattaccgc cgatgctcag ggtattactc tggtgaatat
     1261 tggcgtggga ccatcaaatg ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga
     1321 tgtctggttg atgattggtc actgtggcgg attacgtgaa agtcaggcca ttggcgatta
     1381 tgtacttgca cacgcttatt tacgcgatga ccacgttctt gatgcggttc tgccgcccga
     1441 tattcctatt ccgagcattg ctgaagtgca acgtgcgctt tatgacgcca ccaagctggt
     1501 gagcggcagg cccggtgagg aagtcaaaca gcggctacgt actggtactg tggtaaccac
     1561 agatgacagg aactgggaat tacgttactc agcttctgca cttcgtttta acttaagccg
     1621 ggccgtagca attgatatgg aaagtgcaac cattgccgcg caaggatatc gtttccgcgt
     1681 gccatacggg acactactgt gtgtttcaga taaaccatta catggtgaga ttaaacttcc
     1741 cggtcaggct aaccgttttt atgaaggcgc tatttccgaa catctgcaaa ttggcattcg
     1801 ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat tcgcgtaaat tacgaacctt
     1861 taatgagccg ccgttccgat aataagttct gaaccttctt atcagaaata gatgtgaagg
     1921 agtaagtaag acacctggca aatagcctgc aataatcgtg ggctgtttgc ttccttgggc
     1981 ggatacgagt tttattatcg tcttaatgat ttccacatat taaaagcaag tatgctttca
     2041 aaacacaatt ataaaaaatc ccgccaacaa tataagtttt tataaaatta tggctttaga
     2101 atatttttat ttctaataga cgagattttt cctgttatga tataatatgc cgaattaaca
     2161 catgttaacg atttaccagt aatgtaaata aattttcgag gagatcattc cagtgggacg
     2221 taaatgggcc aatattgttg ctaaaaaaac ggctaaagac ggtgcaacgt ctaaaattta
     2281 tgcaaaattc ggtgtagaaa tctatgctgc tgctaaacaa ggtgaacccg atccagaatt
     2341 aaacacatct ttaaaattcg ttattgaacg tgcaaagcag gcacaagttc caaagcacgt
     2401 tattgataaa gcaattgata aagccaaagg cggcggagat gaaacgttcg tgcagggacg
     2461 ttatgaaggc tttggtccta atggctcaat gattatcgcc gagacattga cttcaaatgt
     2521 taaccgtacg attgctaacg ttcgcacaat tttcaataaa aaaggcggca atatcggagc
     2581 ggcaggttct gtcagctata tgtttgacaa tgctggtgtg attgtattta aaggatcaga
     2641 ccctgaccat atttttgaaa ttttacttga agctgaagtt gatgttcgtg atgtgactga
     2701 agaagaaggt aacattgtta tttataccga acctactgac cttcataaag gaatcgcggc
     2761 tctaaaagca gctggaatca ctgagttctc aacaacagaa ttagaaatga ttgctcaatc
     2821 tgaagttgag ctttccccag aagatttaga aatctttgaa gggcttgttg atgcccttga
     2881 agatgacgac gatgtacaaa aagtttatca taacgtcgca aatctctaat tatcttttaa
     2941 agaaatctgt ctttacggca gatttcttta atctcatata attcttataa aaaatataag
     3001 attcaactcg tcatattgat tatacccccc cgttcccaga gaaataatat ttattaaaat
     3061 tccagttctt ctttttctga ttacagaagg caaagtggca attacgcata gtttcccgat
     3121 aaagacgcga tagcgacatc ccgcataagg catttttctc tttatctttg tacggtactt
     3181 catggaacag agtttttgac cttgcgaatc gtgatgtctg ttggggaggg gcaatttgct
     3241 cactgaagcg tgagactcga ttaagcgcac gaaacacaga aatcaaaaaa cccggtcact
     3301 tttttacaag gtaaccgggt aaaaataatt tttatttttt aactgttttg agactcatag
     3361 agatgtctca aaact
//
LOCUS       DAJVQG010000237         3332 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJVQG010000237 DAJVQG010000000
VERSION     DAJVQG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.243"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(263..463)
                     /locus_tag="OQM83_003876"
     CDS             complement(263..463)
                     /locus_tag="OQM83_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521995.1"
                     /translation="MLCIKFEYLTDKMIKHVSDLLIKEGGFGDACNPKDIFIHATSPN
                     ATLKTAVTAEWFERNKAELGYW"
     gene            complement(463..735)
                     /locus_tag="OQM83_003877"
     CDS             complement(463..735)
                     /locus_tag="OQM83_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1521996.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
     gene            complement(738..1016)
                     /locus_tag="OQM83_003878"
     CDS             complement(738..1016)
                     /locus_tag="OQM83_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521997.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            complement(1017..1574)
                     /locus_tag="OQM83_003879"
     CDS             complement(1017..1574)
                     /locus_tag="OQM83_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1521998.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPSKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
     gene            complement(3138..>3332)
                     /locus_tag="OQM83_003880"
     CDS             complement(3138..>3332)
                     /locus_tag="OQM83_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1521999.1"
                     /translation="LERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQGKT
                     RLQIQEELGISRATYYRLAK"
BASE COUNT          926 a          677 c          617 g         1112 t
ORIGIN      
        1 aattgataca ccggaatcag cgaaagcctg caccatcata gcagtgtcgc tatcaatgaa
       61 aatctgtaat tcatattcgt tcatttttaa aatctccttt ttaccagacc tcctcaatcg
      121 ctctgtaacg agctaaacag tctttttcag gggtagcctg tacagtggta ttacccaccc
      181 taaaataaga tagttttttg cgttagaatg cgattgagag cgtctgataa gctgtttttc
      241 taatttctct taaagtctcg gactaccaat aacctaattc cgctttattt cgttcaaacc
      301 attccgctgt aactgctgtc tttagtgttg cgtttgggct ggtggcatga atgaagatat
      361 cttttggatt gcaagcatca ccgaaaccac cttctttgat gagcaggtct gatacatgct
      421 taatcatttt atctgtcaaa tattcgaatt taatacataa cattaaatct ccaggagtaa
      481 attgtttggg tggcaatttc ttttattacc atccctgaag ataatctttt tagttgcctg
      541 aacttcatga tgcaaaaacc agatgacata cttcagataa taatcacgag tgccataacg
      601 aatcattgga ttaccgtaag catcatctct tgtcttaacc tctccaccat caatccattc
      661 aagcattcct gtctcataat taatcatgaa gtaagttgca aagaaccgtt taatatcatc
      721 tcgccattgc ttcataatca gacctcctga gacagtttgt gaattttgat acttagtaac
      781 cttgctaaat ccagtccaaa agcatttaag gatttcagac tatcaaggtc atcttgctta
      841 atcctgatat ggtgtcttgc agcctctaag cgactattaa gcaaatcctg ttcgttaata
      901 tcacggttaa ttgcaagctt catcactgct gagtgtggca gacctgtaat gtttttgatt
      961 cggttcagtt catcaaaggc tgtctttgta actctggtta atgtgtgttt attcatttaa
     1021 aaatcctctg taactaaata ggggtcaaac tcgaaatctg gttgctcgtt ttccattggc
     1081 tcaatattcc caacggcaat atgatgttca acaatgccaa ttaatttcgc gcacatcaca
     1141 gcaagcaaag cgcaatattt atcgcttggt aattcgtaac taagtctggt tgcctgtcgc
     1201 tgaagatgat ttgtcacggt ggatgcgtta atttcgtcaa gattctttag cagataaaat
     1261 agttttttgt tgtatttcgg gtggccttca cttctacaga acccaataaa aacatcatca
     1321 agcttgcttg gtaactgctc gtaactgtca aagttagcca gataattttt aatagtattc
     1381 tttgttgttt tgcgtactgg cataaatctg tctcgtggcg taatgctacg aatgaccgct
     1441 tcaatgttct tacgttcgga tttgctaaag gttgggactt cgtagctggt ggggttgttt
     1501 gtgtaagcgt aggagttgtg cttgttgcgt tctcttgatg cctgaagcat cgctctaaat
     1561 tctgaatact gcattcatgt tctgactctc ataagatgtt attattctgt taacctatat
     1621 acaatattag cattttcaaa gactaatgtc aagtattttt aacattttcg taacatctgt
     1681 ggtataatgg gtttatgggg agagtaaaat atgaggcgtg gctatgtatt tggtcgcata
     1741 ttttgcattg cacacagacc tcttcaatcg ccctgtaacg agttaaacga cctcttcagg
     1801 tcaactcata aggtcaggta ctttttgtgt cagaatgcga ttgagagcgt cttacaagga
     1861 aaactaatgg taaatctccc tcctgagctg gggtcacctg atgtatggac tggtggaggt
     1921 attgggctga ttacaggcct gctacttcgc tacgtcctcg tcagagtgct tgttgtcttt
     1981 aaactgttcg gtcgtacatg gacgcttgac cttaaggata acgaccacac agacccccgt
     2041 taaactcctt caaaacccct gcacgagatg acagatagaa gtctatggta caggggtgtc
     2101 tttagcattt tgtgctatct attaaaaaaa tagcttgaca gaaccggaaa gccgtatcta
     2161 tcaagcattc acctctctga cagtaatttc taaaagttct tatttttcaa taagttgtaa
     2221 ttagccatat atagagaact aaaagatttt ttagctattt tgtttaaaag catatcaaac
     2281 gatacatttt attgatttag gtcaaagaca aaagcattga caaaatggta agattgttca
     2341 catgacctta cttaacagac ttttaagaga ctttgaaaca gcctgtttaa caggtcacta
     2401 cctctggtct tatcgttccc ttctttctag gtggttatct gttaagagtt tttcagtttt
     2461 tattcttaag ataattgtta agatgatact ctcccttcgg gttatatcgt atctcttctc
     2521 ccgttaaata tcccttcaca gtcagactaa ataacacctg tgtagttctt ggtagaatct
     2581 ttcaaggtaa cttagctctg agtttatgaa gttgacctat gagatagact ctaccgaatc
     2641 tgttagtcaa ttgttgctta ggtgctgcta aggcttactt agtggaaaga gaggggatat
     2701 gtgaccatag gggaacccct cctgttaaaa gactacttag aagtctgagg ttttagtaaa
     2761 atctgtgaat atggcttctc agacgctctg taacgcattc taagcgattt taccccttta
     2821 ttttaaggtg gttagtacat ccctataggt tacccctaaa aatggggtta aaactcgcta
     2881 cagagctttc tatgagatta gcttctaatc gaatcaacta tgaggtttgg taggattgtg
     2941 caaaatctgt acagtaactt ttcagaatct gaaaattttt ataaaaaatg actagccctc
     3001 tttgaatctc atcttccccg aatcttcaaa aatatctgac agatgtctgt agctgctaag
     3061 tacccgtcac accagctatg tgaccccctt ggcaaaaaag ccaaatatca gctacatcca
     3121 tgtaactatt cagtaactta tttagcaagt ctgtaataag ttgctctaga gattcctaac
     3181 tcttcctgaa tctgcaacct cgtcttacct tgtgaaataa gcgtctgtac agcctctgaa
     3241 gaagctttta caggcctccc catatgtacc ccttcagctt tagcacgttc aataccagcc
     3301 tttctacgct ctgctatcaa gcttctttca ag
//
LOCUS       DAJVQG010000238         3315 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJVQG010000238 DAJVQG010000000
VERSION     DAJVQG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.244"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            1..1804
                     /gene="fimD"
                     /locus_tag="OQM83_003881"
                     /pseudo
     CDS             1..1804
                     /gene="fimD"
                     /locus_tag="OQM83_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
     gene            1817..2824
                     /gene="fimH"
                     /locus_tag="OQM83_003882"
     CDS             1817..2824
                     /gene="fimH"
                     /locus_tag="OQM83_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HCS1522000.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPLGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            2835..>3315
                     /gene="sfmF"
                     /locus_tag="OQM83_003883"
     CDS             2835..>3315
                     /gene="sfmF"
                     /locus_tag="OQM83_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HCS1522001.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVE
                     "
BASE COUNT          815 a          751 c          859 g          890 t
ORIGIN      
        1 gtggtgtacg gctttattct tccgataata tgtatccaga tagccagcaa gggtttgccc
       61 caacggtacg tgggattgcc cgtacggcgg cccagctaac gattcggcaa aatggtttta
      121 ttatctatca aagctatgtt tcccccggtg cttttgaaat tactgatttg cacccgacat
      181 cttcaaatgg cgatctggat gtcaccatcg acgagcgcga tggcagaatt acacaattcc
      241 gtattcaaca gtgccgattt tacaacgcga agggcgtttc aaatttgacc tgacggcggg
      301 cgattttcgt agcggtaata gtcagcaatc atcacctttc ttttttcagg gcacggcact
      361 cggtggttta ccacaggaat ttactgccta cggcgggacg caattatctg ccaattacac
      421 cgccttttta ttagggctgg ggcgcaacct cgggaactgg ggcgcagtgt cgctggatgt
      481 gacccatgcg cgcagccagt tagccgacga cagtcgtcat gagggggatt ccattcgctt
      541 cctctatgcg aaatcgatgt acaccttcgg caccaatttt cagttaatgg gttaccgcta
      601 ttcgacacaa ggtttttata cccttgatga tgttgcgtat cgtcgaatgg aggggtacga
      661 atatgattac gattacgacg gtgagcatcg cgatgaaccg ataatcgtga attaccacaa
      721 tttacgcttt agccgcaaag accgtttgca gttaaatatt tcacaatcac ttaatgactt
      781 tggctcgctt tatatctctg gtacccatca aaaatactgg aatacttcgg attcagatac
      841 ctggtatcag gtggggtata ccagcagctg ggttggcatc agttattcac tctcattttc
      901 gtggaatgaa tctgtaggga tccccgataa cgaacatatt gtcggactta atgtttcagt
      961 gcctttcaat gttttgacca aacgtcgcta cacccgggaa aatgcgctcg accgcgctta
     1021 tgcctccttg aacgctaacc gtaacagcaa cgggcaaaat agctggctgg caggcgttgg
     1081 tgggacctta ctggaaggcc acaacctgag ttatcacgta agccagggcg atacctcgaa
     1141 taatgggtac acgggcagcg ccacggcaaa ctggcaggcc acttacggta cgctgggggt
     1201 cgggtataac tacgaccgcg atcaacatga cgttaactgg cagctgtctg gcggtgtggt
     1261 cgggcatgaa aatggcataa cactgagcca gcctttaggg gataccaatg ttttgattaa
     1321 agcgcttggc gcaggcggtg tacgcattga aaatcaaact ggcattttaa ccgactggcg
     1381 cggctatgcg gtgatgccgt atgccacggt ttatcggtat aaccgtatcg cgcttgatac
     1441 caatacgatg gggaattcca tcgatgttga aaaaaatatt agcagcgttg tgccgacgca
     1501 aggcgcgttg gttcgtgcca attttgatac ccgcataggc gtgcgggcgc tcattaccgt
     1561 tacccagggc ggaaaaccgg tgccgtttgg atcactggta cgggaaaaca gtaccggaat
     1621 aaccagtatg gtgggtgatg acgggcaagt ttatttaagt ggtgcgccat tgtctggtga
     1681 attactggtt cagtggggag acggcgcgaa ctcacgctgc attgcgcact atgtattgcc
     1741 aaagcaaagc ttacagcaag ccgtcactgt tatttcggca gtttgcacac atcctcgctc
     1801 ataaaggaaa ttatcaatga agataatctg cagattatta ttggcaatgg catgtttgtg
     1861 tctggcaaac atatcctggg ctactgtttg tgcaaatagt actggcgtag cagaagatga
     1921 acactatgat ctctcaaatg tctttaatag caccaataac cagccagggc agattgttgt
     1981 tttaccggaa aaatccggct gggtaggtgt ctcagcaatt tgtccactcg gcacgctggt
     2041 gaattataca taccgtagtt atgtcactaa ctttattgtt caggaaacta tcgataatta
     2101 taaatatatg caattaaatg attatctatt aggtgcgatg agtctggttg atagtgtgat
     2161 ggatattcag ttccccccgc aaaattatat tcggatggga acagatccta acgtttcgca
     2221 aaaccttccg ttcggggtga tggattctcg tttgatattt cgtttaaagg ttattcgtcc
     2281 ctttattaac atggtggaga tccccagaca ggtgatgttt accgtgtatg tgacatcaac
     2341 gccttacgat ccgttggtta cacctgttta taccattagt tttggtggcc gggttgaagt
     2401 gccgcaaaac tgcgaattaa atgccgggca gattgttgaa tttgattttg gtgatatcgg
     2461 cgcatcgtta tttagtgcgg cagggccggg gaatcgacca gctggcgtca tgccgcaaac
     2521 caagagcatt gcgatcaaat gtacgaatgt tgctgcgcag gcttatttaa caatgcgtct
     2581 ggaagccagt gccgtttctg gtcaggcgat ggtgtcggac aatcaggatt taggttttat
     2641 tgtcgccgat cagaacgata cgccgatcac gcctaacgat ctcaatagcg ttattccttt
     2701 ccgtctggat gcagctgcgg cagccaatgt cacacttcgc gcctggccta tcagtattac
     2761 cggtcaaaaa ccgaccgaag ggccgtttag cgcgctgggg tatttacgcg tcgattatca
     2821 atgaggtacg gagaatgaga agagtactct ttagctgttt ctgcgggtta ctgtggagtt
     2881 ccagtggatg ggcagctgac cctttgggaa cgattaatat caatttgcac ggtaacgttg
     2941 ttgatttctc ctgtaccgta aacactgcgg atattgataa gacggtagat ttaggcagat
     3001 ggcctacgac acaattactg aacgctggcg ataccacggc actcgtccct tttagcctgc
     3061 ggctggaggg atgtcctccg ggttccgttg cgattttatt tacgggaacg ccggcatccg
     3121 ataccaacct gctggctctg gatgatcccg caatggcaca aaccgtcgcc atcgaattac
     3181 gtaatagcga tcgctcccgg ctcgcactgg gggaggcgag cccgactgag gaagtagatg
     3241 caaatggcaa tgtcacacta aacttttttg ccaattatcg agcgttagcc agcggtgttc
     3301 agccaggtgt ggaga
//
LOCUS       DAJVQG010000239         3312 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJVQG010000239 DAJVQG010000000
VERSION     DAJVQG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.245"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            26..592
                     /locus_tag="OQM83_003884"
     CDS             26..592
                     /locus_tag="OQM83_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCS1522002.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            596..1171
                     /locus_tag="OQM83_003885"
     CDS             596..1171
                     /locus_tag="OQM83_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCS1522003.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1213..2265)
                     /locus_tag="OQM83_003886"
     CDS             complement(1213..2265)
                     /locus_tag="OQM83_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCS1522004.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2490..>3312
                     /gene="lpxL"
                     /locus_tag="OQM83_003887"
     CDS             2490..>3312
                     /gene="lpxL"
                     /locus_tag="OQM83_003887"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCS1522005.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGIQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMN"
BASE COUNT          818 a          831 c          817 g          846 t
ORIGIN      
        1 tcgcaaattc gtattggatg cacttatgtc attcacaaat acccctgaac gctatggtgt
       61 tatatctgcg gccttccatt ggctaagcgc aattattgtc tacggcatgt ttgctttagg
      121 tctgtggatg gtcacgctca gttattacga tggctggtat cacaaagcac ccgaactgca
      181 taaaagcatc ggtattttgt taatgatggg gctggttatt cgcgttctgt ggcgtgtcat
      241 atctccaccg ccgggtccgc tgccgagtta ttcgcccatg actcgtcttg ccgcaaaggc
      301 tggacatctc gcattgtacc tattgctgtt cgccatcggc atcagcggct atctgatctc
      361 aactgccgat ggtaaaccga tcagcgtttt tggctggttt gacgtccccg cgacccttgc
      421 cgacgctggc gcacaggcag actttgccgg tgccctgcat ttttggctcg cgtggagcgt
      481 cgtagtactg tccgttatgc acggatttat ggccctgaag catcatttca tcgataaaga
      541 cgacactctg aagcgcatgc tgggaaagtc gtcatctgac tatggagtat aaaaaatgaa
      601 aaaaagcctg cttggtttaa ccttcgcgtc cctgatgttc tctgccggtt cagcggttgc
      661 cgccgattac aaaattgaca aagaaggtca gcacgccttt gttaatttcc gcatccagca
      721 ccttggctat agctggttat acggcacctt taaagatttc gacggtactt ttacctttga
      781 cgaaaaaaat ccggctgccg ataaagtgaa tgtgacaatt aacaccacca gcgtcgatac
      841 taatcacgcc gaacgcgata aacatcttcg cagtgcagat ttcctcaata ccgcaaaata
      901 tccacaggca acattcacct ccaccagcgt gaagaaagac ggtgacgaac tggatattac
      961 cggcgatctg acgctgaatg gcgtaaccaa acccgtcacg ctggaagcga aattaattgg
     1021 tcagggcgac gacccatggg gtggtaaacg tgcaggcttc gaggccgaag gcaaaattaa
     1081 gctcaaggac ttcaatatca agacagattt aggtccagct tctcaggaag tggatctgat
     1141 tatttcagtg gaaggcgtac agcagaagta agcgtgaaat gttgccggat ggcacgcatc
     1201 cggcaatgat atttattctg ttggatcagg aatgcccagt gttgtattca gacgtccacg
     1261 agacttatta aagatcttat tgccattttc acgtcctgcc cgacggcgtc gctgttcttc
     1321 tggcggtaac gcgctttctt cgcagcaaat ctcactacaa caacctttgt atttttccgc
     1381 gcatactgga cactgaataa acagcaggtg gcagccatca tttttacagt tggtatggct
     1441 gtcgcacggc gcgccgcact ggtggcaatg cgcgataatc tcatccgata tacgttcgcc
     1501 catccgctcg tcaaaaacaa aatttttgcc aataaaacgc accggcaagc cctgctcgcg
     1561 cgccttacgg gcgtattcaa taattccgcc ctcgatatgc cagactttgt tgaatccgtt
     1621 atgtttcatc caggcactgg ccttttcaca acgaatgccg ccggtgcagt acatgacgat
     1681 ttttttatct ttatgtgcct gcatcatctc gactgctttt ggcagctgct cacggaaggt
     1741 atctgccgga atttcgagcg cgttttcaaa gtgccccact tcatactcat agtggttacg
     1801 catgtcgata aatagtgcat cgggatcgtc aagcatggcg ttcacttccg ccgcttgcag
     1861 atactcacca acattgctgg catcaaagtg aggatcgtca ataccgtcgg caacaatgcg
     1921 atcgcgtacc ttcatgcgca gtacccagaa ggatttcccg tcatcatcca acgcgatatt
     1981 caggcgtaaa ccctctaaag ccgggtcgaa ggcatagagc tgcgcgcgaa atgtttcaac
     2041 attgctcgca ggtacgctga tttgcgcgtt aatgccctca tgcgccagat acactcgccc
     2101 aaaaacattc agcgcggtaa acagctgata taaagcgtca cgggtcgcct taggatcggc
     2161 gatgtggaaa tacttgtaaa acgaaatggt ggttcgcggt tcgctctcaa ccaacatttt
     2221 ggcttttaac gcgtcgttgg aaatgcggtt gtgtaacact ggcatggtgt acggttcctg
     2281 cgagatggga aagtaaaaat ccgcggcatg atatagcaat tatcgataat taacatccac
     2341 acattttacg ctacatttgc gcattaaaaa ttatttgtta tttacaagcg cggcaatttc
     2401 gcccagtctt cagccacaaa ttttggttgc gggcgaaaaa atgcgacaat acatacaatt
     2461 gcccgaatag gttgaaaaac aggattgata tgacgaatct acccaagttc tccaccgcac
     2521 tgcttcatcc gcgttattgg ttaacctggt tgggtattgg cgtactttgg ttagtcgtgc
     2581 aattgcccta cccggttatc taccgcctcg gttgtggatt aggaaaactg gcgttacgtt
     2641 ttatgaaacg acgcgcaaaa attgtgcatc gcaacctgga actgtgcttc ccggaaatga
     2701 gcgaacaaga acgccgtaaa atggtggtga agaatttcga atccgttggc atgggactga
     2761 tggaaaccgg tatggcgtgg ttctggccgg accgccgaat cgcccgctgg acggaagtga
     2821 tcggcatgga acacattcgt gacgtgcagg cgcaaaaacg cggcatcctg ttagttggca
     2881 tccattttct gacactggaa ctgggcgcgc ggcagtttgg tattcaggaa ccggggattg
     2941 gcgtttaccg cccgaacgat aatccactga ttgactggct acaaacctgg ggccgtttgc
     3001 gctcaaataa atcgatgctc gaccgcaaag atttaaaagg catgattaaa gccctgaaaa
     3061 aaggcgaagt ggtctggtac gcaccggatc atgattacgg cccgcgctca agcgttttcg
     3121 tcccgttatt tgccgttgag caggctgcga ccacgaccgg aacctggatg ctggcacgga
     3181 tgtccggcgc atgtctggtg cccttcgttc cacgccgtaa gccagatggc aaagggtatc
     3241 aattgattat gctgccgcca gagtgttctc cgccactgga tgatgccgaa actaccgccg
     3301 cgtggatgaa ca
//
LOCUS       DAJVQG010000240         3285 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJVQG010000240 DAJVQG010000000
VERSION     DAJVQG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.246"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..229)
                     /locus_tag="OQM83_003888"
     CDS             complement(<1..229)
                     /locus_tag="OQM83_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522006.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKK"
     gene            403..1116
                     /locus_tag="OQM83_003889"
     CDS             403..1116
                     /locus_tag="OQM83_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCS1522007.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSPLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            1148..1906
                     /locus_tag="OQM83_003890"
     CDS             1148..1906
                     /locus_tag="OQM83_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522008.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCLSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1952..>3285
                     /locus_tag="OQM83_003891"
     CDS             1952..>3285
                     /locus_tag="OQM83_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCS1522009.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
BASE COUNT          751 a          790 c          853 g          891 t
ORIGIN      
        1 atttttttgc tgttttcacc tgatttcgtt caattgtttt atgtaaggat tttcccatca
       61 acggtaattt tgtcactgca cgaccgacat ttccggcctg ttttcctaaa tgaactcttt
      121 cggcagcacc atatttttcg acaacggtaa taagatgttc acacaccgtt gacttgcctg
      181 aaccatcgct accaataacg gcaattaatg gaggtgttat tgactgcatg aatatatcct
      241 ttaatttaaa catccattaa aaatatttat ttggttaata tgtttttatg aaagcgtaat
      301 tcaggtcaat gtcacaatta accattgtca caataaggtt gaacggatat tctgagcacg
      361 gaccacgtaa tattcaatac attatttact gccgggtttt tcgtgaatca acgtaatatg
      421 tcaataatta attccacgcc agtgcgtgtt attgccattg taggatgtga tggttcaggt
      481 aaatcgcccc tcacggcaag cctggtaaat gaactggcag caagaatgcc aacagaacac
      541 atttatctcg ggcaatcgtc cgggctaatt ggcgaatgga tttcacagct ccctgttatt
      601 ggcgcacctt ttgggcgtta tctgcgaagt aaagcggcac atgtgcacga aaaaccctca
      661 acaccgcctg gcaatattac tgctctggtt atctatctgc tttcctgctg gcgggcgtac
      721 aagtttcgca aaatgttgtg taaaagccag caaggctttc tgctcatcac cgaccgctac
      781 ccgcaggttg aagtgccggg ttttcgcttt gatggcccgc aattggcaaa aaccacgggc
      841 ggtaacggtt ggataaaaat gttaaggcag cgcgagctga agctgtacca atggatggca
      901 tcttatttgc ccgtattgtt gattcgtctt ggcattgatg aacaaaccgc gtttgcgcgt
      961 aaacctgacc accaactggc agcgttacag gaaaaaatcg ccgttacgcc gcaactgact
     1021 ttcaacggcg ccaggatcct tgaactggat ggtcggcaac cagccgatga aatcctgcaa
     1081 gcgtcactac gtgcaattca cgccgcgctt tcctgatcac caaaatggat aggggatgat
     1141 ttaacgaatg gatgcactac aaacacaaac tgttaatagt acaaccgcgc cgcagcccag
     1201 ctacattccg gggctaattg cggtggtcgg gtgcgatggc accggtaaat ccacgctgac
     1261 caccgatctg gtgaaatcat tgcaacagca ctggcaaacc gagcggcgct atctggggct
     1321 gctctcgggc gaagacggcg acaaaatcaa acgattaccg ttggttggcg tctggctgga
     1381 acggcgactg gcggccaaat cctcgaaaac ccaaagcatg aaaaccaaat ctccggcgct
     1441 atgggcggcg gtgattatgt actgcttatc gctgcgaaga atggcgaatc tacgcaaggt
     1501 tcagcgactg gcgcaaagtg gcgttctggt ggtcagcgat cgcttcccgc aggctgaaat
     1561 ttcgggcttt tattacgacg gaccggggat tggcgtcgaa cgtgcgaccg ggaaaatcag
     1621 catgtttctg gcgcagcgcg aacggcgttt ataccaacaa atggcgcaat atcgcccgga
     1681 attaattatt cgcctgggca ttgatattga gactgccatc tcccgcaagc ctgaccatga
     1741 ctatgccgag ctgcaggaca aaatcggtgt catgtcgaag attggctata acggcacaaa
     1801 aattcttgag attgattcca gagcgcccta cagcgaagtg ctggaacagg cacagaaggc
     1861 ggtttctctg gttgccatcg tttctgaccg ccgaagttta acttaaggct ggcgggatac
     1921 tttttactat caacgataag gtcaggctga attggcgggt tttaacatca aacattggtt
     1981 tgcagatggc gcgtttcgca ccattattcg caatagcgcc tggttaggct ccagtaatgt
     2041 cgtgagcgcc ttgctggggc tgttggcgct ctcgtgtgcc ggtaaaggga tgacgcccgc
     2101 catgtttggc gtactggtga ttgtgcaatc gtacgccaag tcgatcagcg attttattaa
     2161 gtttcagaca tggcagctgg tggttcagta cggaacgcca gccttgacca acaataatcc
     2221 gcagcaattt cgcaatgtcg tctcattttc cttctcgctg gatatcgtca gcggcgcggt
     2281 ggcgattgtc ggcggcattg ccttactgcc attcctttcc cattcattag gtctggatga
     2341 ccaaagcttt tggctggcgg cgctgtattg cacgctcatt ccttcaatgg cttcctccac
     2401 gccgaccggc attctgcgtg cggtagatcg cttcgattta attgctgtac agcaggcgac
     2461 gaaacctttt ctgcgcgcag cggggagcgt cgtagcctgg tattttgact ttggttttgc
     2521 gggttttgtt attgcctggt acgtatcgaa tctggttggc ggcaccatgt actggtggtt
     2581 tgcagcgcgc gaattacgcc gcagaaatat ccataatgcc ttcaaattga atctgtttga
     2641 gtctgcccga cacatcaaag gcgcgtggag ttttgtctgg tcaaccaaca ttgcccactc
     2701 catctggtcg gcgcgtaact cgtgcagcac agtgctggtg gggatcgtgt taggacccgc
     2761 tgccgccggg ttatttaaaa tcgccatgac attctttgac gccgccggaa cgccagcggg
     2821 tttgctgggt aaaagttttt acccagaggt gatgcgttta gatccgcgca ccaccagacc
     2881 gtggttgtta ggtgtgaagt ccggtttact ggcgggcgga atcggtattc tggtggcgct
     2941 cgcggtgttg atcgccggca agccgctcat ttcgctggtg tttggcgtta agtatctcga
     3001 agcgtatgac ctgattcagg tgatgctggg cgcaattgtg atttcgatgc tgggtttccc
     3061 gcaggagtca ttgctattga tggcgggaaa acagcgcgct tttctcgtgg cgcaaaccat
     3121 cgcctcaatc ggctacatcg tattgctgtt catgttctgt catctgtttg gtgtgctggg
     3181 agcagcgttt gcttacttcg gtgggcaatg tctggatgtg gtgctctcgt taattccaac
     3241 tttaaaggca ttttttcagc gtcattcctt gctttataac gcagc
//
LOCUS       DAJVQG010000241         3273 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJVQG010000241 DAJVQG010000000
VERSION     DAJVQG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.247"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..283
                     /locus_tag="OQM83_003892"
     CDS             <1..283
                     /locus_tag="OQM83_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCS1522010.1"
                     /translation="VYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKK
                     YFGDGTGVGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            503..1285
                     /gene="hisJ"
                     /locus_tag="OQM83_003893"
     CDS             503..1285
                     /gene="hisJ"
                     /locus_tag="OQM83_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCS1522011.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            1375..2061
                     /gene="hisQ"
                     /locus_tag="OQM83_003894"
     CDS             1375..2061
                     /gene="hisQ"
                     /locus_tag="OQM83_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCS1522012.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            2058..2774
                     /locus_tag="OQM83_003895"
     CDS             2058..2774
                     /locus_tag="OQM83_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1522013.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            2782..>3273
                     /locus_tag="OQM83_003896"
     CDS             2782..>3273
                     /locus_tag="OQM83_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCS1522014.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIA"
BASE COUNT          787 a          764 c          876 g          846 t
ORIGIN      
        1 ggtctattcc gatctggctg caggacgtct ggatgctgcg ttacaagatg aagttgctgc
       61 cagcgaagga ttcctcaagc aacctgctgg taaagctttc gcctttgctg gctcatcagt
      121 aaaagacaaa aaatacttcg gtgatggcac cggtgtaggg ctacgtaaag atgatgctga
      181 actgacggct gccttcaata aggcgcttgg cgagctgcgt caggacggca cctacgacaa
      241 gatggcgaaa aagtatttcg actttaatgt ctacggtgac tgatacgtcg ctgggaagct
      301 gtacctgatg gaatgatcat catggtgcac gccaggtttg ttgcactatc gtggtgcatt
      361 gaaatgcata cttaagcatt tttaatgaaa aataatacgt ctaacggggg gaatattttg
      421 ccttgatggt caattttatg gcacgataag tgtaacaaac ctgtaaatat tccctataaa
      481 aagactgtca gttgaggaca ttatgaaaaa actggtgcta tcgctctctc tggttctggc
      541 cttctccagc gcaactgcgg catttgctgc gattccgcaa aacatccgca tcggtaccga
      601 cccgacctat gcgccatttg aatcaaaaaa ttcacaaggc gaactggttg gcttcgatat
      661 cgatctggca aaggaattat gcaaacgcat caatacgcaa tgtacgtttg tcgaaaatcc
      721 gctggatgcg ttaatcccgt ccttaaaagc gaagaagatt gacgccatca tgtcatcgct
      781 ttccattacg gaaaaacgtc agcaagaaat agccttcacc gacaaactgt acgctgccga
      841 ttctcgtttg gtggtggcga aaaattctga cattcagccg acagtcgagt cgctgaaagg
      901 caaacgggta ggcgtattgc agggcaccac ccaggagacg ttcggtaatg aacattgggc
      961 accaaaaggc attgaaatcg tctcgtatca ggggcaggac aacatttatt ctgacctgac
     1021 tgccggacgt attgatgccg cgttccagga tgaggtcgct gccagcgaag gtttcctcaa
     1081 acaacctgtc ggtaaagatt acaaattcgg tggcccgtct gttaaagatg aaaaactgtt
     1141 tggcgtaggg accggcatgg gcctgcgtaa agaagataac gaactgcgcg aagcactgaa
     1201 caaagccttt gccgaaatgc gcgctgacgg tacttacgag aaattagcga aaaagtactt
     1261 cgattttgat gtttatggtg gctaatcgcc atcggtgaaa gaaagcccgc tcccttcggg
     1321 taaccggagg gagacaagag gcggtaattc accacacacg acaggacagg cagcatgttg
     1381 tatgggtttt caggtgttat tttacagggt gcgctcgtca cgctggagct ggctatcagc
     1441 tctgtagtgc tcgctgtaat catcggttta attggcgctg gcggtaagct ctcgcaaaat
     1501 cggctttcgg ggctgatttt cgaagggtac accacgctga ttcgtggcgt gccggactta
     1561 gtgttgatgc tgctgatttt ctacggtttg cagattgcgc taaacacggt gacggaggcg
     1621 atgggcgtcg ggcagattga tatcgatccg atggtcgctg gtattatcac tctcggtttt
     1681 atctacggtg cttattttac cgaaacgttc cgtggtgctt ttatggcagt gccgaaagga
     1741 catatagagg cggcgacggc gttcggtttt actcgtgggc aagtgtttcg gcggatcatg
     1801 tttccggcga tgatgcgtta cgccctgcca ggcattggca acaactggca ggtgatcctc
     1861 aaatctaccg cactggtttc gttactcggc ctggaagatg tggtcaaagc cacccaactg
     1921 gcaggcaaaa gtacctggga accgttctat ttcgccatcg tctgtggcgt gatttacctg
     1981 gttttcacca ctgtttccaa tggtgtgctg ctgttccttg agcgccgcta ctccgtgggt
     2041 gtgaagaggg ctgacctgtg atcgaaatct tacatgaata ctggaaaccg ctgctgtgga
     2101 ccgacggtta tcgctttact ggtgtggcga tcactctgtg gctgcttatt ttgtcggtag
     2161 tgataggcgg agtcctggcg ctgtttctgg cgattggtcg tgtctccagt aataaataca
     2221 tccagtttcc aatctggtta tttacctata tttttcgcgg tacgccgctg tatgttcagt
     2281 tgctggtgtt ctattccggc atgtacacgc ttgagattgt taagggaacc gaattcctta
     2341 acgctttctt ccgcagtggc ctgaactgta ccgtgctggc gctgacgctt aacacctgcg
     2401 cttacactac cgagattttt gctggggcaa tccgttcggt tccgcatggg gaaattgaag
     2461 ccgccagagc ctatggcttc tcgactttta aaatgtatcg ttgcattatt ttgccttctg
     2521 cgctgcgtat tgcgttaccg gcatacagca acgaagtgat cctgatgctg cactctactg
     2581 cgctggcgtt tacggccact gtgccggatc tgctgaaaat agcccgcgat attaacgccg
     2641 ccacgtatca accttttacc gccttcggca ttgccgcggt gctctattta atcatctctt
     2701 atgtcctgat cagcctcttt cgcagagcgg aaaaacgctg gttgcagcat gtgaaacctt
     2761 cttcaacgca ctgagaatac gatgtccgag aataaattaa acgttatcga tttgcacaaa
     2821 cgctacggcg aacatgaagt gctgaaaggg gtctcactgc aagcgaatgc cggagatgta
     2881 ataagcatca tcggatcgtc gggatcgggg aaaagtacct ttctgcgctg cattaacttc
     2941 ctcgaaaaac cgagtgaagg gtcgatcgtg gtcaatggcc agacgatcaa tctggtgcgc
     3001 gacaaagacg gtcaactcaa agtcgccgat aaaaatcaac tgcgcttact gcgcacacgc
     3061 ctgacgatgg tattccagca cttcaatctc tggagccata tgacggtgct ggaaaacgtc
     3121 atggaagcgc cgattcaggt gttgggcctg agcaagcagg aagcgcgcga gcgggcggtg
     3181 aagtatctgg cgaaagtcgg gatagacgaa cgtgcgcagg ggaaatatcc ggtgcatctt
     3241 tccggcggtc agcaacagcg tgtttctatc gcg
//
LOCUS       DAJVQG010000242         3197 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJVQG010000242 DAJVQG010000000
VERSION     DAJVQG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.248"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(10..636)
                     /gene="dsbA"
                     /locus_tag="OQM83_003897"
     CDS             complement(10..636)
                     /gene="dsbA"
                     /locus_tag="OQM83_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCS1522015.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(653..1639)
                     /gene="srkA"
                     /locus_tag="OQM83_003898"
     CDS             complement(653..1639)
                     /gene="srkA"
                     /locus_tag="OQM83_003898"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCS1522016.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1716..1985)
                     /locus_tag="OQM83_003899"
     CDS             complement(1716..1985)
                     /locus_tag="OQM83_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCS1522017.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2055..2639
                     /gene="mobA"
                     /locus_tag="OQM83_003900"
     CDS             2055..2639
                     /gene="mobA"
                     /locus_tag="OQM83_003900"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCS1522018.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2621..3148
                     /gene="mobB"
                     /locus_tag="OQM83_003901"
     CDS             2621..3148
                     /gene="mobB"
                     /locus_tag="OQM83_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCS1522019.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          833 a          774 c          771 g          819 t
ORIGIN      
        1 tacattgaat tattttttct cggacagata tttcactgta tcagcatact gctgaacaaa
       61 aacatccata ttgctggtat ccataccctg cggattcagc tgatatttac cgttaacaaa
      121 catcgccgga acgccacgca attgcacgtc agctgcagct ttttcctgct gagcgaccag
      181 agatttgacc acgaagctgt tccacgccgc gtcgtactct tcacctttaa tacctgcgtt
      241 gataaataca tcgcggatat cagatgctga acgaatggtc tgggttttct gtacgccttc
      301 aaacagcgga acagtgactt tgtcttccac gcccagcgcc atcgccacag cccatgcctg
      361 agtcagatct ttgcccaggt caccacccat gaagttgacg tggtatttag tcatcttcac
      421 gccttccggc agttttttct tcacattatc agaaatatgc agaacttctt caaactgata
      481 gcagtgcgga cagaagaaag agaaaaactc cagcacttgc ggcgcgccag ctaccggttt
      541 ttccagggta gtgtactgtt taccatcttc atactgcgcc gccgatgcgc taaacgctaa
      601 aactaaacca gccagcgcca gccaaatctt tttcatgatc tactctctcc gattaataca
      661 taggtgttaa ttgtaaaggg ggttcttgta gaacttttgc ctgttctata aaagtcgccg
      721 tttgtcgcag ccagtaatct tccccggtta accacgggaa atttttcggg aacgcgggat
      781 cagcccaacg ccgcattagc caggcaagat aataaaccaa acgcatggcg cgtaaaggtt
      841 caatcagtcc gatttcagcg gtgtcgaact cgccaaattc ttcataagct tcaataatag
      901 tttccagttg catccgctgc tcggctttat cgccattgag caacatccac aaatcctgaa
      961 tagctggacc attacgtgca tcatccagat caacaaacat tggaccatcg cgccagagaa
     1021 tattcccggc gtggcagtct ccatgtagcc gcagaacggt gaaatcttcc cgccagtgtg
     1081 ctgtaacggc ggcgatcagc tcatctgtcg ctttcaggaa tgccgctttc aacccggaag
     1141 gtatcagtgt agcgtcctca aacagcttgc gtggctcaat gagatattca ttcagaccga
     1201 tggtcgggcg atggataaaa agctgtttgt gccccgtctg gtgcatacgc cctaaataac
     1261 gcccaaccgc ttccgtctga tcgatattat cagcttcgaa ctggcgacca ccgacgcttg
     1321 gaaaaacagc gaaataaaat ccctgatgat tcaataaagt ctgaccgtta aaggccacag
     1381 gtgctgcgac cggaacttca tcatttacca gctgcaacgc aaattgatgt tcttcgagga
     1441 tttgatcggc tgtccaacgt tcagggcgat aaaatttgac gacaaaacgt cgacgctctt
     1501 cgtcctgaaa ttgatagaca cggttttcat agctgttaag cggggtaaga ccggaatcca
     1561 cccggatccc ttgctcaaac agagcgtcca tgatggtatc cgggtgtagt gtctggaaag
     1621 taaaagcgct gttgttcatc ccatcatccg gaaaacatta cgaatgatga aggatatcat
     1681 ttcacgatga ttttggtggc gccgcttaca agcttttact ctttaattac gccgcgagcg
     1741 cgtaacagcg ccgttttgaa gtcttcttca taatctttct gcaaacccgg gatcaccgca
     1801 tctttcgcgg aatcacgcat tttcaggtga tagatcagaa tgtcatccgt caaatccgcc
     1861 agttcgccgt caaaacctga ctctttcgcc agtttctgca aaaattgcaa caggttaagg
     1921 tctggctctt tttgccaggc tggctggagg agttcaataa cttcattcag acgtttacat
     1981 ttcatagtga tgctccttac tcttgagaca gacacgttag cagggtcaat cccacaataa
     2041 aagaggcgat atcggtgaat ctgatgacga cgataacagg cgttgtgctg gcaggcggta
     2101 aagccagacg aatgggcggc gtagataaag gattgcttga attaaacggc aaaccattat
     2161 ggcaacatgt cgctgacgcg cttatgacgc agctctctca cgtcgtggtt aatgccaatc
     2221 gtcatcagga aatctatcag gcaagcggtc tgaaagtgat tgaagattca ctggcggatt
     2281 acccaggccc tctggcagga atgctttcag taatgcagca ggaagctggt gagtggtttt
     2341 tgttttgtcc gtgcgatacg ccttacattc cccatgtttt agcttcccgg cttaatcatc
     2401 agcgcaaaga tgcgcctgtc gtgtgggtcc atgacggtga acgcgatcac ccgactattg
     2461 ctctggtaaa ccgtgctatt gagcctttat tactagaata tctgcaagca ggagaacgcc
     2521 gggtaatggc atttatgcgt ctggctggcg gccatgcggt tgatttcagc gatcataaag
     2581 atgcatttgt taacgtgaat acgccagagg agcttgcccg atggcaggaa aaacgatgat
     2641 accgttactc gcctttgccg cgtggagtgg taccggaaaa actacgcttt tgaaaaaact
     2701 gatcccggca ttatgcgcca gagggatccg tccagggctg attaagcata cgcaccatga
     2761 tatggatgtt gataagccag gcaaagatag ctatgagctg cgcaaggctg gcgcggcaca
     2821 aaccatcgtt gccagccagc aacgatgggc cttgatgacg gaaaaaccag acgaagaaga
     2881 gctggatcta cagtttctcg caagtcgaat ggatacctca aagctggatt tgattctggt
     2941 cgaagggttt aagcatgaag agatcgcaaa gattgtgctg tttcgcgatg gagccggaca
     3001 tcgacctgaa gaattagtga tagacaggca tgttattgct gtagccagtg atgtgccgct
     3061 taatcttgat gtcgcattac tggatattaa tgatgttgag gggctggctg attttgtggt
     3121 ggagtggatg caaaagcaga acggataagc aagagtaggc tggataagac gcgccagtgt
     3181 cgcatccggc atttttt
//
LOCUS       DAJVQG010000243         3193 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJVQG010000243 DAJVQG010000000
VERSION     DAJVQG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.249"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            181..2049
                     /locus_tag="OQM83_003902"
     CDS             181..2049
                     /locus_tag="OQM83_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HCS1522020.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDI
                     KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKNLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
     gene            2086..>3193
                     /locus_tag="OQM83_003903"
     CDS             2086..>3193
                     /locus_tag="OQM83_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCS1522021.1"
                     /translation="MYLTDNIIKLSNKIGQRVYTVVSEMSIEAWITKEPQPFVYRRSG
                     AYQKLTIGSDWGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGI
                     TNKASSYGVPPDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIAT
                     RNQLLKSLYYDIEVLFDWINDGQKFESIHPKGITPEQFIAKRSERTDEIISTLEYIDN
                     TLITFSNEEIIKCQIAIQSIINKNNNPSEFRIMATGHAHLDIAWMWPLREGRRKAIRT
                     FATALANIEKYPDYIFGASQYQLFHWIKKDYPYFFEKLKKQIAAGRFELQGCFWVECD
                     LNLVSGESLIRQIMHGTRFTLQNFSKKINYVWQPD"
BASE COUNT         1009 a          571 c          630 g          983 t
ORIGIN      
        1 cagatgccaa tgcattaata aatactttgc atgaatctgg caatgatatg gtgcaggatg
       61 aaaaacattt gagtgatagc aaaaaccgtt aatattttcc tttcattgtg atcgtctgta
      121 ggcgatcaca atctatttgt cttttataac gtctaaactt ttttatgcaa aggtgtattt
      181 atgatttttt ttaacgatat aaagcccact ggttggttaa agaataagat ggaatggtat
      241 atgaacggtc atataggaga actggataaa ttagttcccc atcttattac ggagcataaa
      301 atatatggaa gagatcgtat aactccacgc actgtatctg ctgaagatgg cgttattaaa
      361 aaacagaata atcccgatga taacatgatg caatattatt ggtgggatag tgaaactcaa
      421 tcgaattgga aggatggata ttgccgcagt gcctacttgt tagataataa agaatggcag
      481 gaaaaagcga gcgatttatg tttggaactt atcgatacgc aagatgatga tggctacatt
      541 ggaatttatg attcatcatt aaaatttaac tgtaaagcag aaaacggaga gttctgggca
      601 caagctactg ccttgcgttt tgtgcttgga tgttatgagt ccacccgtaa tgagagttta
      661 ttaacttctc ttacccaagc cgcacaatgc attatggctg gctaccctaa agaaacgtca
      721 catcctttcg ttgtggaaca gggttttgct ggacatagcc acggattaac aataacagat
      781 gttttctacc gcctttatga aataaccaat aacattgaat atctctcata ttgcttgtgg
      841 ctgtatgaag attattccgc aggatgcgtg tcggagcaag atctgcaata taaaaatgtc
      901 atagaccctg gttaccttct taaaggtcat ggcgtacata cgtatgagca tattagggca
      961 ttagccatcg cagcgagcca acggccgcag tatcaaactg cattagatat ttttttatcc
     1021 cagttgaaat attatcttac accatctggc gctcccattg gtgatgagtg gattacagga
     1081 cgcacagcta atgccactta tacaggctat gaatattgtt ctgttcatga actatttagc
     1141 acttatgtta tgttaataaa actgaccact aatattggtc tggccgatga tgctgagtgg
     1201 ctattctata atgctagttt tggcatgcag catcccatac ttcacgcgat aatgtactgt
     1261 aagactgata attgttatga agcaaatgaa aggaaacatc ctgacagtaa tcagtttaat
     1321 accagatata aatactcccc tactcatgag gaggctgctg tctgctgcgt accgaataca
     1381 gcaagaacca taccggcttt tgtcgaaaat atgtttcaag aaaataacaa tggttttact
     1441 gcattatttt atggcccgtg tgagttttat ggcacattta ataatgttgc aattaaaatt
     1501 acacaattaa ctgaataccc tcatgattta agcgtaaaat gtctcattga gcctgagtca
     1561 tctgttcgtt ttgctctcag tttccgttac cccggctggg caaaaatgat gattattaat
     1621 ggtgttactt ttactaaaaa tgatactcaa aactcactta ttattttaga tagaacatgg
     1681 caacatcatg atgtgattga cattaaattt atagctgata tacagtttaa tactgactta
     1741 tgtggtgaca cttatatttc cagaggtccc gttttatatg caattgagct tgagagcgat
     1801 attttaatca aaaaaaacct gctgaacggg aaatattttg actcaggata cataccctgt
     1861 agtagagctg atgaatctat ccagttctct tatgctgata tggaatcttt ttcatattca
     1921 acctcaccag agggcaaaca atacattgaa ggctgttttt atttaaacaa gactaaaata
     1981 gtcagaaaac ttataccatt tggtcaaacc attcttcgca aagtaacttt ctccaatata
     2041 gagagttaat attttcgagt gttataatta attaaaggga tttctatgta ccttactgat
     2101 aatattatca aactgtcgaa taaaataggt cagcgtgttt atactgttgt ctctgaaatg
     2161 tcgattgaag catggataac aaaagaaccg cagccttttg tttatcgccg tagcggcgct
     2221 taccagaaac tcaccattgg ctcagactgg ggtcaactgt ttgactgtgc ctggatgcga
     2281 gtttatggta aacgtccagc agataaattt agacacaaac tcgtcgctct cgttgatatc
     2341 ggtggcgaag gtcttattgt ggataagaat ggttcaccca tctgcggcat cacgaataaa
     2401 gcatcatctt atggcgtacc gccagataaa ccaggtaaat gggtggttga cctttctctc
     2461 gttagtgaaa atgacgaggt tgagttttgg attgatgctg cctgcaacga tttatttggc
     2521 tatgttacta atggagggat tattactgat gttcatatcg cgacacgtaa tcaactactg
     2581 aaatcccttt attatgatat tgaggtgctt ttcgactgga ttaatgatgg tcagaagttt
     2641 gaatccatac atcctaaagg tatcacccca gaacaattta ttgccaaaag aagtgagcgt
     2701 actgacgaaa tcataagtac tctcgaatat attgataata cgttaattac ctttagcaat
     2761 gaagaaataa ttaaatgtca aattgcaatc caatccataa tcaataaaaa caataatcct
     2821 tcagagttca ggattatggc tactggtcat gcccatttag atatagcgtg gatgtggcct
     2881 ttaagagaag gacgcagaaa agcaataaga acatttgcca ccgcccttgc aaacattgaa
     2941 aaatatcctg attacatttt tggcgctagt cagtaccaac tttttcactg gataaaaaaa
     3001 gattatccct atttcttcga aaagttaaaa aaacaaatcg ccgcaggccg ctttgagtta
     3061 caaggttgct tctgggtcga atgcgattta aatctggtaa gtggtgaatc gctgataagg
     3121 caaatcatgc atgggacacg attcacactg cagaattttt caaaaaagat aaattatgtc
     3181 tggcaaccgg atg
//
LOCUS       DAJVQG010000244         3172 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJVQG010000244 DAJVQG010000000
VERSION     DAJVQG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.250"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            650..1366
                     /gene="nanC"
                     /locus_tag="OQM83_003904"
     CDS             650..1366
                     /gene="nanC"
                     /locus_tag="OQM83_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCS1522022.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1386..2492
                     /gene="nanM"
                     /locus_tag="OQM83_003905"
     CDS             1386..2492
                     /gene="nanM"
                     /locus_tag="OQM83_003905"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCS1522023.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2557..>3172
                     /locus_tag="OQM83_003906"
     CDS             2557..>3172
                     /locus_tag="OQM83_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCS1522024.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRR"
BASE COUNT          957 a          651 c          715 g          849 t
ORIGIN      
        1 tcactgagtt agcttgcaaa gcttcccttg tttagaccct aaataaattt tttataaaac
       61 aaacagttaa aagaaatcct ttagtttttg aagaccacct caagtgttcg tctggcttca
      121 catggcatct tcctcttaga aaaagatcga catattttgt gacacgaatt gcaaatctcg
      181 ttttgttgta tggcttgcgt gatttttgat ctggtataac aggtataaag gtgcaccgcg
      241 atagtcaatg agacagggca tctcgcaatc tatggcaaac atcacttcag ttctttctca
      301 tcgggtgatg aaaacgcact tcagtctgaa aggaatatga aaatgagatc aacagacact
      361 ctattttatg actctgggta aaatggattg agtaagtgat atagcttacg aacattcaaa
      421 tcaattaaac atcagaagag attttatact caggtattta atctgattct ctgtttattt
      481 aaataatgtg gaaagagaat tttcacagga gaccttatac aaaaatataa aataagctac
      541 ccgttgccaa agacactata agcctggcaa aaaatattac acaacataaa tgctaattgt
      601 ttatgcgggc tttgtattgc tttctgtatc ctacaaatga gtgaaattta tgaaaaaggc
      661 taaaatactt tctggcgtat tattactgtg cttttcgtca ccattaattt ctcaggctgc
      721 gacactggac gtacgtggtg gatatcgtag cggaagccat gcctatgaga ctcgactcaa
      781 agtcagtgag ggatggcaaa atggatggtg ggcaagcatg gaaagtaata cctggaatac
      841 cattcatgat aataaaaagg aaaatgccgc actcaatgat gttcaggttg aagttaatta
      901 cgcgattaaa cttgatgatc aatggacggt gcgcccggga atgttaacgc attttagcag
      961 caacggcaca cgctacggac cctacgtaaa actgtcctgg gacgcgacaa aagatcttaa
     1021 ttttggcatt cgctatcgtt acgactggaa agcttaccga caacaagact tatccggtga
     1081 tatgtctcgt gataacgttc atcgttggga tggatatgtc acttaccata ttaatagtga
     1141 tttcaccttc gcatggcaaa cgacgctata cagcaaacag aatgattatc gctatgcaaa
     1201 ccacaagaaa tgggcgacgg aaaatgcctt tgttctacaa taccatatga cgcccgatat
     1261 tacgccatac atagaatatg actaccttga ccgccagggt gtttacaacg gcagagataa
     1321 tttatcggaa aacagttatc gcattggtgt gtcatttaaa ctgtagtaga caggagacag
     1381 tcacaatgaa taaaactata atggcgcttg ctatcatgat ggcttcattt gccgcaaacg
     1441 cgtctgtatt accggaaact cctgtgccat ttaaaagtgg taccggagca attgataacg
     1501 acactgtcta cattggttta ggtagcgcag gtacagcatg gtacaagcta gatacacagg
     1561 ccaaagataa aaaatggact gcgttagctg cattccctgg tggaccgaga gatcaagcaa
     1621 cctctgcatt tattgatggc aatctatatg tgtttggcgg cattggcaaa aacagcgagg
     1681 gattgactca ggtatttaat gacgtacaca aatacaaccc caaaaccaat agctgggtta
     1741 aattgatgtc gctcgcgccg atgggcatgg cgggtcatgt gacttttgta cacaacggca
     1801 aggcttatgt tactggcggt gttaaccaga atatcttcaa tggctatttt gaagatctca
     1861 acgaggctgg aaaagattca accgctatag ataaaatcaa cgcccactat tttgacaaaa
     1921 aagcagaaga ttatttcttc aataagtttc tgttgtcttt tgatccctca acacagcaat
     1981 ggagttacgc tggcgaatcg ccctggtacg gaacggctgg tgcggcggtt gtgaataaag
     2041 gtgataaaac ctggcttatt aatggcgaag ccaaaccagg attgcgaacg gatgccgtat
     2101 ttgaacttga tttcaccggt aataatttaa aatggaataa gcttgatccc gtctcatcac
     2161 cagatggcgt cgctggcggt tttgcgggga taagcaatga ttctcttata tttgccggag
     2221 gggccggatt caaaggttca cgagaaaatt accagaacgg taagaactat gcgcatgaag
     2281 gcctgaaaaa atcatatagc actgatattc atctttggca taacgggaaa tgggataaat
     2341 cgggtgaatt atcgcaaggt cgggcctacg gagtatcatt gccctggaat aatagtttat
     2401 taattattgg cggtgaaact gcaggcggca aagcggtgac ggattcagtt ttaatttctg
     2461 tgaaggataa taaagtcaca gtacaaaact aacgcttcag ggccccggta aaggggccct
     2521 gctatcaatt tgtcatttgt attacgcgaa aattatatga acgcaataat atcgcccgat
     2581 tattactatg ttcttaccgt tgctggtcag tctaatgcca tggcgtatgg cgaaggactg
     2641 ccattaccgg acagggaaga tgcgcctcat tccagaatta aacaattagc gagatttgcg
     2701 catacgcatc ccggaggccc gccatgtcac tttaacgaca ttattccact gactcactgc
     2761 ccacacgatg ttcaggatat gcagggttat caccatcctc tggcaacgaa tcatcaaaca
     2821 cagtacggca ccgttggcca ggcactgcat attgcacgga aattactgcc cttcattcct
     2881 aataatgcag ggattcttat cgttccgtgt tgccgtggcg gatcggcttt taccgcgggc
     2941 agcgaaggga catattcaga acggcacgga gccagccatg atgcttgtcg ttggggaacg
     3001 gatactccgc tgtaccagga tttagtcagc agaacgcgag ccgcactggc aaaaaatccg
     3061 cagaacaaat tcctcggcgt atgctggatg caaggcgaat ttgacttaat gaccagtgac
     3121 tacgcgtcac accctcaaca ctttaatcat atggttgaag cctttcgtag gg
//
LOCUS       DAJVQG010000245         3165 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJVQG010000245 DAJVQG010000000
VERSION     DAJVQG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.251"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            75..1340
                     /locus_tag="OQM83_003907"
     CDS             75..1340
                     /locus_tag="OQM83_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1522025.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            1352..2218
                     /gene="ydiB"
                     /locus_tag="OQM83_003908"
     CDS             1352..2218
                     /gene="ydiB"
                     /locus_tag="OQM83_003908"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCS1522026.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            2249..3007
                     /gene="aroD"
                     /locus_tag="OQM83_003909"
     CDS             2249..3007
                     /gene="aroD"
                     /locus_tag="OQM83_003909"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCS1522027.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
BASE COUNT          812 a          708 c          773 g          872 t
ORIGIN      
        1 tatagtaatt atgtaaaccg tcggagaaca atacgtacgg taacgaaatt atctttcagc
       61 aaggagctgt gaaaatgtct caaaataagg ctttcagcac gccatttatc ctggctgttc
      121 tttgtattta cttcagctat ttcctgcacg gcattagtgt tattacgctt gcccaaaata
      181 tgacatctct ggcagaaaag ttttccactg acaacgcggg cattgcctat ttaatttccg
      241 gtatcggttt ggggcgattg atcagtattt tattcttcgg tgtgatctcc gataagtttg
      301 gtcgtcgggc ggtgatatta atggcagtaa taatgtatct gctattcttc tttggtattc
      361 ccgcttgccc gaatttaact ctcgcctacg gtctggcagt gtgcgtaggt atcgctaact
      421 cagcgctgga tacgggtggc taccccgcgc tcatggaatg ctttccgaaa gcctctggtt
      481 cggcggtcat actggttaaa gcgatggtgt catttgggca aatgttctac ccaatgctgg
      541 tgagctatat gctgctcaat aatatctggt acggctatgg gctgattatt ccgggtattc
      601 tgtttgtact gatcacgctg atgctgttga aaagcaaatt ccccagccag ttggtggacg
      661 ccagcgtagc taatgaatta ccgcaaatga acagcaaacc gttagtctgg ctggaaggtg
      721 tttcatcggt actgttcggt gtagccgcat tctcgacctt ttatgtgatt gtggtgtgga
      781 tgcccaaata tgcgatggct tttgctggta tgtcagaagc tgaggcatta aaaaccatct
      841 cttattacag tatgggctcg ttggtctgtg tctttatttt tgccgcacta ctgaaaaaaa
      901 tggtccggcc catctgggct aatgtattta actctgcgct ggcaacaata acagctgcca
      961 ttatctacct gtacccttct ccactggtgt gtaatgccgg cgcctttgtt atcggtttct
     1021 cagcagctgg cggcatttta cagctcggtg tttcggtcat gtcagagttt ttccccaaaa
     1081 gcaaagccaa agtcaccagt atttatatga tgatgggtgg actggctaac tttgttattc
     1141 cgctgattac cggttatctg tcgaacatcg gcctgcaata tatcattgtt ctcgatttta
     1201 ctttcgcgct gctggccctg attaccgcaa ttattgtttt tagccgctat taccgcgttt
     1261 ttattattcc tgagaatgat gtgcggtttg gcgaacgcaa atttagcacc cggttaaaca
     1321 caattaagca tagaggttaa aggagttaat tatggatgtt accgcaaaat acgaattgat
     1381 tgggttgatg gcttatccta tccgccacag tttatcgcct gaaatgcaga ataaagcctt
     1441 agaaaaagcg ggattgccat ttacctatat ggccttcgaa gtggataacg atagctttcc
     1501 cggagcaatt gaaggattaa aagccctcaa aatgcgcgga actggtgtgt cgatgccgaa
     1561 caaacaactg gcgtgtgaat atgttgatga attaacgcca gcagccaaac tggtgggtgc
     1621 catcaacacc atcgttaatg atgatggcta tctgcgtggc tataacaccg acggcacggg
     1681 ccatattcgc gccattaaag agagcggttt tgatatcaaa ggcaaaacga tggtgctgtt
     1741 aggggccgga ggtgcctcaa cagcgattgg cgcgcagggg gcaattgaag gtttaaaaga
     1801 aattaaactc tttaaccgtc gggatgagtt cttcgataaa gccctcgcct tcgcgcagcg
     1861 ggttaacgaa aacaccgatt gtgtcgtcac ggttaccgat ctcgccgatc agcaagcctt
     1921 tgctgaagtc ctggcttccg ccgacatttt aaccaatggc acaaaagtag gtatgaaccc
     1981 ccttgagaat gaatcattgg ttaatgatat cagtctgtta catccgggac tgctggtcac
     2041 tgaatgcgtg tataacccgc atatgacgaa gttattgcag caggcacaac aagcaggttg
     2101 caaaacgatt gatggatacg gcatgttgtt gtggcaaggg gctgaacagt tcacgttatg
     2161 gactggcaaa gatttccctc tggaatatgt taaacaggtc atggggttcg gtgcctgaca
     2221 ggctgaccgc gtgcagaaag ggtaaaaaat gaaaaccgta actgtaaaag atctcgtcat
     2281 tggtgcgggc gcacctaaaa tcatcgtctc gctgatggcg aaagatatcg cccgcgtgaa
     2341 atccgaagct ctcgcctatc gtgaagcgga ctttgatatt ctggaatggc gtgtggacca
     2401 ctttgccgac ctctccaatg tggagtctgt catggcggcg gcaaaaattc tccgtgaaac
     2461 catgccagaa aaaccgctgc tgtttacctt ccgcagtgcc aaagaaggcg gcgagcaggc
     2521 gatttccacc gaggcttata ttgctctcaa tcgtgcagcc atcgacagcg gcctggttga
     2581 tatgatcgat ctggagttat ttaccggcga tgatcaggtt aaagaaaccg tcgcctacgc
     2641 ccacgcgcat gatgtgaaag ttgtcatgtc caaccatgac ttccataaaa cgccggaagc
     2701 cgaagaaatc attgcccgtc tgcgcaaaat gcagtccttc gacgccgata ttcctaagat
     2761 tgcgctgatg ccgcaaagta ccagcgatgt gctgacgttg cttaccgcga ccctggagat
     2821 gcaggagcag tatgccgatc gtccaatcat cacgatgtcg atggcaaaaa ctggcgtaat
     2881 ttctcgtctg gctggtgaag tatttggctc ggcggcaact tttggtgcgg taaaaaaagc
     2941 ctctgcacca gggcaaatct cggtaaatga tttacgcacg gtattaacta ttttacacca
     3001 ggcataagca ataatatttc ggcgggaaca ccctccccgc cgaacttaaa aaatatattt
     3061 aaccgtattt aataaaaata tttcgtgagt ctctgtgcgc taattctcca tctggcgtag
     3121 ggaaaatcac atctgaatca ggaattaaca atgaaacctg taaaa
//
LOCUS       DAJVQG010000246         2994 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJVQG010000246 DAJVQG010000000
VERSION     DAJVQG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.252"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            18..1076
                     /locus_tag="OQM83_003910"
     CDS             18..1076
                     /locus_tag="OQM83_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCS1522028.1"
                     /translation="MAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTV
                     FTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACH
                     SLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFR
                     DHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKS
                     SEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDH
                     LVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKK
                     SFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="OQM83_003911"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="OQM83_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCS1522029.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2994
                     /locus_tag="OQM83_003912"
     CDS             2805..>2994
                     /locus_tag="OQM83_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylanase"
                     /protein_id="HCS1522030.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSL"
BASE COUNT          839 a          632 c          745 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc ttag
//
LOCUS       DAJVQG010000247         2967 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJVQG010000247 DAJVQG010000000
VERSION     DAJVQG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.253"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..2661
                     /gene="lacZ"
                     /locus_tag="OQM83_003913"
     CDS             <1..2661
                     /gene="lacZ"
                     /locus_tag="OQM83_003913"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HCS1522031.1"
                     /translation="QTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGK
                     NRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPSTQISDFHVATHFNDDFSRAV
                     LEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVEN
                     PALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVN
                     RHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRFGLYVVDEA
                     NIETHGMVPMNRLTDDPHWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDAL
                     YRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGE
                     TRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSA
                     YGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFR
                     HSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIELPELPQPESAGQLWLTVHVVQP
                     NATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQLTTSETDFCIELGNKRWQFNRQ
                     SGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAE
                     AALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTP
                     HPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSEN
                     GLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGI
                     GGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
     gene            2713..>2967
                     /locus_tag="OQM83_003914"
     CDS             2713..>2967
                     /locus_tag="OQM83_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1522032.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLV"
BASE COUNT          665 a          790 c          831 g          681 t
ORIGIN      
        1 cagacgcgaa ttatttttga tggtgttaac tcggcgtttc atctgtggtg caacgggcgc
       61 tgggtcggtt acggccagga cagccgtttg ccgtctgaat ttgacctgag cgcattttta
      121 cgcgccggaa aaaaccgcct cgcggtgatg gtgctgcgct ggagtgacgg tagttatctg
      181 gaagatcagg atatgtggcg aatgagcggc attttccgtg acgtctcgtt gctgcacaaa
      241 ccgagcacac aaatcagcga tttccatgtt gccactcact ttaatgatga tttcagccgc
      301 gcggtactgg aggcagaagt tcagatgtac ggcgagctgc gcgatgagct gcgggtgacg
      361 gtttctttgt ggcagggtga aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa
      421 attatcgatg agcgtggtgg ttatgccgat cgcgtcacgc tacgtctgaa cgtcgaaaac
      481 ccggcgctgt ggagcgccga aatcccgaat ctctatcgtg cggtggttga actgcacacc
      541 gacgacggca cgctgattga agcagaagcc tgcgatgtcg gtttccgcga ggtgcggatt
      601 gaaaatggtc tgctgctgct gaacggcaag ccgttgctga ttcgcggcgt taaccgtcac
      661 gagcatcatc ctctgcatgg tcaggtcatg gatgagcaga cgatggtgca ggatatcctg
      721 ctgatgaagc agaacaactt taacgccgtg cgttgttcgc attatccgaa ccatccgctg
      781 tggtacacgc tgtgcgaccg cttcggcttg tacgtggtgg atgaagccaa tattgaaacc
      841 cacggcatgg tgccaatgaa tcgtctgacc gatgatccgc actggttacc cgcgatgagc
      901 gaacgcgtaa cgcgaatggt gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg
      961 ctggggaatg agtcaggcca cggcgctaat cacgacgcac tctatcgctg gatcaaatct
     1021 gtcgatccat cccgcccggt gcagtatgaa ggcggcggag ccgacaccac ggccaccgat
     1081 attatttgcc cgatgtacgc gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa
     1141 tggtccatca aaaaatggct ttcgctacct ggagagacgc gcccgctgat cctttgcgaa
     1201 tacgcccacg cgatgggtaa cagtcttggc ggcttcgcta aatactggca ggcgtttcgt
     1261 cagtaccccc gtttacaggg cggcttcgtc tgggactggg tggatcagtc gctgattaaa
     1321 tatgatgaaa atggcaatcc gtggtcggct tacggcggtg attttggcga tacaccgaac
     1381 gatcgccagt tctgcatgaa cggtctggtc tttgccgacc gcacgccgca tccagcgctg
     1441 acagaagcaa aacaccagca gcagtttttc cagttccgtt tatccgggca aaccatcgaa
     1501 gtgaccagcg aatacctgtt ccgtcatagc gataacgagc ttctgcactg gtcggtggcg
     1561 ttggatggca agccgctggc aagcggtgaa atgcctctgg atgttgctcc acaagataaa
     1621 cagttgattg aactgcctga actaccgcag ccggagagcg ccggacaact ctggcttacg
     1681 gtacacgtag tgcaaccgaa cgcgaccgca tggtcagaag ccggacacat cagcgcctgg
     1741 cagcagtggc gtctggcgga aaacctcagc gtgacactcc ccgccgcgcc ccacgccatc
     1801 ccgcaactga ccaccagcga aacggatttt tgcatcgagc tgggtaataa acgttggcaa
     1861 tttaaccgcc agtcaggctt tctttcacag atgtggattg gcgctgaaaa acaactgctg
     1921 acgccgctgc gcgatcagtt cacccgtgca ccgctggata acgacattgg cgtaagtgaa
     1981 gcgacccgca ttgacccgaa cgcctgggtc gaacgctgga aggcggcggg ccattaccag
     2041 gccgaagcag cgttgttgca gtgcacggca gatacacttg ccgacgcggt gctgattacg
     2101 accgctcacg cgtggcagca tcagggaaaa accttattta tcagccggaa aacctaccgg
     2161 attgatggta gtggtcaaat ggcgattacc gttgatgttg aagtagcgag cgatacaccg
     2221 catccggctc ggattggcct gacctgccag ctggcgcagg tagcagaacg ggtaaactgg
     2281 ctcggattag ggccgcaaga aaactatccc gaccgcctta ctgccgcctg ttttgaccgc
     2341 tgggatctgc cattgtcaga catgtatacc ccgtacgtct tcccgagcga aaacggtctg
     2401 cgctgcggga cgcgcgaatt gaattatggc ccacaccagt ggcgcggcga cttccagttc
     2461 aatatcagtc gctacagcca acaacaactg atggaaacca gccatcgcca tctgctgcac
     2521 gcggaagaag gcacatggct gaatatcgac ggtttccata tggggattgg tggcgacgac
     2581 tcctggagcc cgtcagtttc agcggaattc cagcttagcg ccggtagcta ccattaccag
     2641 ttggtctggt gtcaaaaata ataataaccg ggcaggccat gtctgcccgt atttcgcgta
     2701 aggaaatcca ttatgtacta tttaaaaaac acaaactttt ggatgttcgg tttattcttt
     2761 ttcttttact tttttatcat gggggcctac ttcccgtttt tcccgatttg gctacatgac
     2821 atcaaccata tcagcaaaag tgatacgggt attatttttg ccgctatttc tctgttctcg
     2881 ctattattcc aaccgctgtt tggtctgctt tctgacaaac tcgggctgcg caaatacctg
     2941 ctgtggatta ttaccggcat gttagtg
//
LOCUS       DAJVQG010000248         2964 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJVQG010000248 DAJVQG010000000
VERSION     DAJVQG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.254"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..216
                     /locus_tag="OQM83_003915"
     CDS             <1..216
                     /locus_tag="OQM83_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HCS1522033.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTRVQGTVVMFSASGKK"
     gene            complement(271..369)
                     /locus_tag="OQM83_003916"
                     /pseudo
     CDS             complement(271..369)
                     /locus_tag="OQM83_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(415..597)
                     /locus_tag="OQM83_003917"
     CDS             complement(415..597)
                     /locus_tag="OQM83_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1522034.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGYEQIFEDKLSGTRTGRPG
                     LKRALKRLQKGDALVV"
     gene            696..1586
                     /locus_tag="OQM83_003918"
                     /pseudo
     CDS             696..1586
                     /locus_tag="OQM83_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1589..2122
                     /locus_tag="OQM83_003919"
     CDS             1589..2122
                     /locus_tag="OQM83_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1522035.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(2151..2678)
                     /locus_tag="OQM83_003920"
     CDS             complement(2151..2678)
                     /locus_tag="OQM83_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1522036.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(2680..>2964)
                     /locus_tag="OQM83_003921"
     CDS             complement(2680..>2964)
                     /locus_tag="OQM83_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1522037.1"
                     /translation="ALGSGAQVASSGDIYGSVWENNWLSTWLHNHVVRDIRLGSIEYK
                     NVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYNVGSV"
BASE COUNT          836 a          661 c          649 g          818 t
ORIGIN      
        1 gatggaactc tatacggagc cacaattctt ggcgatggag ttgtgcacaa tagcttcggt
       61 caaaaactga tgcgcattta caatcagaaa ggcatctttt caaacacaaa agattccgaa
      121 gaaggcttaa cacatatact ctcagagcat ttcgagaatg ttaaaaccag ggttcaaggt
      181 actgtagtaa tgttttccgc ttcagggaaa aaatagcatc caaccgcagc acgttcttgc
      241 ttaagacgtg ctgcggcata atcccaatga ttactccctg acagggttcg taggccactc
      301 aatatcaggt gcagttgatg tatcaacacg gttcagcaac acccgatact ttttctaggc
      361 ttccaccacc agcacgacaa gatgccgcat acagtgcccc agtcagtcca gttttcagac
      421 aaccagtgcg tcaccttttt gaaggcgctt taaagcacgt tttaatccag gtcggcctgt
      481 ccttgttccg cttaatttat cttcaaatat ttgttcatat cctgcacaaa caagagcgtt
      541 tcgttgcagg tctgtattct ggtcatttgt tgataccctt acatagccaa tcagcacgct
      601 gaatctcccg tccaaaagca caaatcatgc catgcaggcc agaaaccgcc attatctaaa
      661 acctcggttt acgagaactc ggcacaagcg gggagaaaat accgttactc agtacagcga
      721 atacctggac taatcgacaa acattcagcg gtggcctttc tggtgaacta tccggcaatg
      781 cttctacagc tgcaaaatta aaaactgcca ggaaaataag caatgtggct tttgatggtt
      841 cctccgatat cacattaaaa gcaagtcatg ttggtgcgtt tgccttaggg aaaacaggaa
      901 gcaccgttgc gaatgataaa gcagttggat ggaactggag tagcggagcc tataacgcaa
      961 ctattagtgg tgcatcaacg ttaattattc atttttatat gggagaagga agttgtcctg
     1021 cagctcagtt tcggattaat tataaaaatg gcggtatttt ttatcgttca gcccgtgatg
     1081 gttatggttt tgaagccgac tggtccgaat tttacaccac cagaaaacct tcagcaggag
     1141 atgttggtgc actgccgtta tctggtggtc aactgaatgg tgcactgggt atcggaacat
     1201 ccagtgctct tggcggtaat tcgattgtat tgggtgataa tgacacgggc tttaaacaaa
     1261 aaggcgatgg taatctggat gtttatgcta ataacgtcca tgttatgcgc tttgtctccg
     1321 gaagcattca aagtaataaa accataaata ttacgggacg tgttaatccc tcggattacg
     1381 gtaactttga ttcccgctat gtgagagatg tcagacttgg cacacgagtt gtttagacca
     1441 tgaagaaagg cgtgatgtat gaaaaatcag gccatgtaat cacggggctt ggtattatcg
     1501 gtgaagtcga tggtgatgat ccggcagtat tcagaccaat acaaaagtta attaacggaa
     1561 catggtataa cgtatcgcag gtataatcat gcagcattta aaaaacattg tcgcaggtaa
     1621 tccaaaaacc gttgaacaat accagctaac aaagaatttt gatgttatct ggttatggtc
     1681 cgaagatgga aaaaattggc atgaggaatt aaaaaacttt caggaggata caataaaact
     1741 ggcttacaca gttgagggaa taattattgc tatagacaag gatgtatcag caattaatcc
     1801 agaaggttta agtgtcgttg agttgcctga tattacagca aatcgccggg ctgatatttc
     1861 gggcaactgg atgttcaaag atggtgcggt gataaagcga acttataccg aggaagagca
     1921 aaggcaacaa gcggaaaatg aaaagcagag cctgctgcag ctcgtcaggg ataaaaccca
     1981 gctgtgggac tctcagctac agctgggcat catttccgat gagaataaac aaaaattaac
     2041 cgagtggatg ctctatgcgc agaaggttga atccacagac acctccagcc tgccagtaac
     2101 gtttcccgaa caaccagaat gagagaaggc ccgctatcgg gccttaattt ttattcaggc
     2161 ttttgtggcc attcaggatt tgccgtatcc acacggctga ccagaacact gtagcgttcc
     2221 catgcttcca gtcgtgtgcg ttcctcgtct gttgccatat tcagcctgac agctcgttcc
     2281 agcggctgga tgactgattc agcttcggaa agcaatgcgg ccttttgtga ttcggcctgt
     2341 tgttgcagtt cctgttctgt atattccctc tttactactt taccatccag aaacatccat
     2401 tttccgttat tgtctgcgtg acgatttgcc gtaatatcag gaacttcaac tatgcttagc
     2461 ccttcaggat taatagttga aacgtcttta ctgacagcaa cgatgatgtt gttctcatcg
     2521 taagcaattt ttatggtgtc tggctgaaaa ttcttttgtt cctcatacca gtttttgcca
     2581 tcttcgcaaa agagccatac cacgcccgct tttttagtta gctggtattg ttccggtgtt
     2641 ttcggattcc cggccttaat atttttcagg tgcatcatct taaacactcc ccacgttgta
     2701 ccatattccc ccaatcaatt tctgaattgg ccttctggca acagtgtcga caatatcatc
     2761 cgcattgctg ttaattgcgg ctgttaaaac ataacctgac gcatcgccaa agccgtagtc
     2821 gcgccatacg tttttatatt caatgctgcc aagacgaata tcccgaacga catgattatg
     2881 cagccatgtg cttaaccagt tgttttccca cactgaacca taaatatcgc cactagacgc
     2941 aacttgcgca ccacttccca gcgc
//
LOCUS       DAJVQG010000249         2937 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJVQG010000249 DAJVQG010000000
VERSION     DAJVQG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.255"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            35..262
                     /locus_tag="OQM83_003922"
     CDS             35..262
                     /locus_tag="OQM83_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCS1522038.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(263..637)
                     /locus_tag="OQM83_003923"
     CDS             complement(263..637)
                     /locus_tag="OQM83_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HCS1522039.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(720..1766)
                     /gene="mdtG"
                     /locus_tag="OQM83_003924"
                     /pseudo
     CDS             complement(720..1766)
                     /gene="mdtG"
                     /locus_tag="OQM83_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2097..2672)
                     /locus_tag="OQM83_003925"
     CDS             complement(2097..2672)
                     /locus_tag="OQM83_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1522040.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          762 a          695 c          687 g          793 t
ORIGIN      
        1 ttatgccggt atttttttac ggaagaggta tcaaatgcgt ttaattgtgg tgagcatcat
       61 ggtgaccttg ctgagtggct gtggcagcat cattagccgc actataccgg ggcaggggca
      121 cggcaaccaa tattatcccg gtgtgcaatg ggatgtgcgt gactccgctt ggcgttatgt
      181 cacaatcctt gatctgccat tctccctggt ttttgatact ttactgctgc cgatcgacac
      241 tcatcatggc ccgtatgagt gattaacgtt catcccactc atcagctgca gcgtgtcctt
      301 cctcggtatc cagcggtggc tcaagctgaa attccccctc gtcccattca tgtaatgtat
      361 tctcttcctg ccactcctgg cggatctcta tctcatcata gtcgccatca aaaacacttt
      421 gcgcggcttc accgctaagc ataggtaaac attcaccttc ttccccttcg tcggcaaaaa
      481 actcaacttg ccacatgatg tcgccgtcct gcaaaacgta tttttgggca ttgaactgtt
      541 gcacattcgc atcttcggcg tcgatgccgg ggttgtctgc aagaaattct tcgcgtgcag
      601 cgtcaatggc ttcttcaagc gttgcgtaca tggtcatcac gattctccct ttgagttgat
      661 gaggtttcag ggaaaaggat agctgattct ccgcttttgc aagtatgaaa ggcgaaaaat
      721 cagttcgata cctggggtat tcgacgacga cgtagactgt tccatgaata gactgcgttg
      781 aataacacta cgccagcggt gacgagaaat accgctctga aaccgtagtt cgctgaaatc
      841 gctgctccca tcaatggtcc ggtaacgttg ccaatatcac gaaacgattg gttatagctg
      901 aagatacgtc cggcgatttg gttgctcgag ttataaacta acagtgtctg tacggcgggg
      961 agtagtgcac catcggcggc accgagcaaa aaacgtaaaa tcccaagttg caatggcgtc
     1021 tgaacgtaag acattgggat caacagcagt acagaaaaga tcagcgccgt aatcaggatc
     1081 ttttcgggtc cgattcgatc gccaagtttg ccgagtcgtg gtgcacttag cagagccgcc
     1141 acgcctggca ccgaggcgat catgccactg ataaaggcga cgttactgac gttacccgcc
     1201 agttcgcgga catacagcgt cagaatgggg gcaattgagc ccgtcgccac ctggatgatt
     1261 aacgtagtga caaacaggct gagtaccagt ttcgggtttt taagtgatgt caccacttcc
     1321 cgcatgtgca gcatctcttt tttgctgatc ggctggaatt tttctcttat gcaaaacagg
     1381 gtgacgaaaa agcagagtat gagcacactg gcggtaataa agaataccgg acgtaagccg
     1441 tagctatcgg cgagcaggcc gccagccatt gggccgagca acgcaccact aacgccgcct
     1501 gtggagagcg tacccagcgc ccagccgctt ttattacgcg gtacttgtgt ggcgataaga
     1561 gcattagcgt tggggacaaa tccgccaagt aacccaagaa gcgcccgcag gatcaaaaac
     1621 tgccagatat tttgtgccag ccccatcaac accatcacga tgcccatgcc gagggcagag
     1681 cgtaatagca tgagttttcg gcctttacgg tcggcgagtc caccccaaaa cggtgaggcg
     1741 atggccgaaa ataaaaatgt aatgctcata tatgctatct taatgcacta cctgataagt
     1801 gagcctatac aggttattgc tgtatgttta tacagtatta ttggtagaag actgtgtaga
     1861 tttggtatag agatgaatag atactattaa ggtaacttat tgaaatcaat aaagattctt
     1921 gtgcagtctg aatagataac ttagcgttat gtctagtcgt ttccctctcc attcctaaaa
     1981 agtgctgtat actggcctat acaacgctat aaagacctct acatagcgcc ttgatagcta
     2041 tgtcacaggt acaaaaaagc cccctataaa ggaggctgtg tagtctatta agggtcttac
     2101 agggtcttgg tccagtgtct gctaacagtc cttacagaga ggttaagaac cttagctacc
     2161 tgagactgag atagtcccct ttccttgtac tctaagaccg ctctagttgt gtcactcgct
     2221 ataggtcttc caagtacctt accttgaact ctagcacgct ctagaccctg tttagtgcgc
     2281 tctctgatac ggtttctttc aaactctgca aaggtactaa atagctgtag aaccaactta
     2341 ccctctgagc ttgaaaggtc tttaacgggt aaatctaaac agatgagctt cacaccctta
     2401 tcattaagaa gggtcactgt attctgtagg tcgatgttat cccttcccag acggtcaagt
     2461 ttaagaacaa caagggtgtc accttcttct agcttatggt tgaccagatt cgcaaatccg
     2521 tccctatcca tagctgacac tgaaccagag acaacctcct ctataactcg tgacttagga
     2581 atatcatagc cagccttctt aatggcttga atctggttct ctgtggtgga agtctctact
     2641 gatacacggc agtaagcaaa ggttcgtgac atagatatcc ctctgggtag tcattaaacg
     2701 gttagtttgt cttgtctata gacattaaag caaatggtag atttgttgtc tatagctata
     2761 gtgactcttc tatgtagaca ttaaacggac gtttcttgtc gtgggtcaca tagatacttt
     2821 tagggcttct gtcaagtgat attcgtagat tttgcaaggg ggaggtgtaa ggctgaaaca
     2881 gggctactaa gaacacatac atctaaggct ggaaagtacc tgagcagata ttcttca
//
LOCUS       DAJVQG010000250         2922 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJVQG010000250 DAJVQG010000000
VERSION     DAJVQG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.256"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..1061
                     /locus_tag="OQM83_003926"
     CDS             <1..1061
                     /locus_tag="OQM83_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HCS1522041.1"
                     /translation="MFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLLQSNFS
                     VGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFFAGIFF
                     SISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRNFWVFV
                     IFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAIIPFFV
                     NRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKYSVANF
                     DKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLLFGIFF
                     LSKKREQIVMETPVPSAI"
     gene            complement(1590..1790)
                     /gene="torI"
                     /locus_tag="OQM83_003927"
     CDS             complement(1590..1790)
                     /gene="torI"
                     /locus_tag="OQM83_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HCS1522042.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(1913..2257)
                     /locus_tag="OQM83_003928"
     CDS             complement(1913..2257)
                     /locus_tag="OQM83_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522043.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGALH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2499..>2922
                     /locus_tag="OQM83_003929"
     CDS             2499..>2922
                     /locus_tag="OQM83_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522044.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTS"
BASE COUNT          708 a          608 c          672 g          934 t
ORIGIN      
        1 tgatgttcta cggcatcgtt caggataaac tcggtctgaa gaaaccgctc atctggtgta
       61 tgagtttcat cctggtcttg accggaccgt ttatgattta cgtttatgaa ccgttactgc
      121 aaagcaattt ttctgtaggt ctaattctgg gagcgctctt ttttggcctg gggtatctgg
      181 cgggatgcgg tttgcttgac agcttcaccg aaaaaatggc gcgaaatttt catttcgaat
      241 atggaacagc gcgcgcctgg ggatcttttg gctatgctat tggcgcgttc tttgccggca
      301 tattttttag tatcagtccc catatcaact tctggttggt ctcgctattt ggcgctgtat
      361 ttatgatgat caacatgtgt tttaaagata aggatcacca gtgcgtagcg gcggatgcgg
      421 gaggggtaaa aaaagaggat tttatcgcag ttttcaagga tcgaaacttc tgggttttcg
      481 tcatatttat tgtggggacg tggtctttct ataacatttt tgatcaacaa ctttttcctg
      541 tcttttatgc aggtttattc gaatcacacg atgtaggaac gcgcctgtat ggttatctca
      601 actcattcca ggtggtactc gaagcgctat gcatggcgat tattcctttc tttgtgaatc
      661 gggtagggcc aaaaaatgca ttacttatcg gtgttgtgat tatggcgttg cgtatccttt
      721 cctgcgcgct gttcgttaac ccctggatta tttcattagt gaagctgtta catgccattg
      781 aggttccact ttgtgtcata tccgtcttca aatacagcgt ggcaaatttt gataagcgcc
      841 tgtcgtcgac gatctttctg attggttttc aaattgccag ttcgcttggg attgtgctgc
      901 tttcaacgcc gactgggata ctctttgacc acgcaggcta ccagacagtt ttcttcgcaa
      961 tttcgggtat tgtctgccta atgttgctat ttggcatttt cttcctgagt aaaaaacgcg
     1021 agcaaatagt tatggaaacg cctgtacctt cagcaatata gacgtaaact tttccggttg
     1081 ttgtcgatat ctccctatcc ctcaaccgga aaataataat actaaagtgc ttagcccggc
     1141 taataatcac ctaatccaaa cgcctcattc atgttctggt acagtcgctc aaatgtactt
     1201 cagatgcgca gttcgctgat ttccaggaca ttgtcgtcat tcagcgacct gtcccgtgta
     1261 tcacggtcct gcgaattcat caaggaatgc attgcggagt gaagtatcga gtcacgccat
     1321 atttcgctat caggattctg tgtgatggtt acatcgcccg gcccagggct gtttagtcat
     1381 cagcgctttc tgacagtgct gagatttcaa cctgttgcag taaaaatgag tagatataag
     1441 gcaagtgtgc tgccaaaccc atcttttacg gggtgaaggt agatttcgtt tgaagggtat
     1501 ctggtgtccc ctgcagacat ctacttgagg cggcagggga ttgattggaa tgctgttttt
     1561 tagatttgaa aaatatttta cccgctattt tacccattgg cgcggcttaa gagcttattt
     1621 ttgaattcac aatggtcacg atataaccat cttgctcgtc cgtggataac tttggcttta
     1681 ggcaggtcgc cggacttaat ccggtcatag atgaaggtct taccgaagcc agtatcagcc
     1741 atgatgaatt tcaaatcaac cagtgaatca ggttgtagtt cgtgttgcat gagtgctatc
     1801 tccgaatatg gaatcgaacc tgcaaatcag gcaataaaaa gccgcattga tgcagcaatg
     1861 gtaggtctgg atatcttgag aaatgaacag gcctcatcga gtgtgaggcg ggttagtcct
     1921 tgcgtagctc gctgattctt ctgtaagtct ctggtgcttt gttcccgtac gtcttcattt
     1981 cagacttcaa cagagcaacg agtgaatccc attcgttgag gattcctttg aatgccggaa
     2041 cgcgctttgc aaccttgtcg aatgaatctc tgatttctgg aatctgctca acaagtgcaa
     2101 cgcatcgccg aaagtctgct gcgtcatgga gagcgccgaa gtgatgacca tagatattct
     2161 ttttcaggcc acatgcgatt gaggcaagag ttgcgctact gatgccgaca tcgccagttg
     2221 attgccattt caaaaccttc atagccaaat ctgacatttc ttgtctccat aaaacaaaac
     2281 tcgccgtagc gagctcagat aaaagaaatc cccgtcagtg cgaggatgct gttcattgct
     2341 gctatacact tttttgctct caacgtaagc ggtagcccat tctgttgggt tggtcagttg
     2401 cttttaggaa atgctcttta ccccttaaac gtcggctgaa agagctaaaa tccatgcaaa
     2461 aaatttacgc aattttgtgt attattgtgc agtaagtaat gagctatttt ctgtgcaaaa
     2521 aatggatgat aaatttgtcc ggggcaggaa aaattttatg ggcgctaaac atgaaaaaag
     2581 attcgtatcc ttatttgatt tgcatgacag tttcagggct gatctttatt ttccttttct
     2641 tctggtggcg ggcagatatc tacagggtca cgtttcttaa tcagagtata tcccactatt
     2701 acattctgtt tagcatggga atagcttttc tgttatctct gttttgggtt aagaagggga
     2761 tagtaaaaca aagcggctgg aagagtctgt cagcatacct taaggtttat gcagggatat
     2821 gcatattttc tggatttttt ctgattatac cccttacaac actaacttat tttttgcctg
     2881 gagagacatc gtcttatgtt gcaccgtatc ggtatacttc cg
//
LOCUS       DAJVQG010000251         2903 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJVQG010000251 DAJVQG010000000
VERSION     DAJVQG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.257"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..143)
                     /locus_tag="OQM83_003930"
     CDS             complement(<1..143)
                     /locus_tag="OQM83_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522045.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVL"
     gene            complement(189..530)
                     /locus_tag="OQM83_003931"
     CDS             complement(189..530)
                     /locus_tag="OQM83_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522046.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(699..890)
                     /locus_tag="OQM83_003932"
     CDS             complement(699..890)
                     /locus_tag="OQM83_003932"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522047.1"
                     /translation="MGPALGAARLAQIAANPEKSLIELLPQLPLEQSHLPDAQRYAAY
                     QPRRETFRRLYQQLLPLMA"
     gene            complement(823..2154)
                     /gene="xylB"
                     /locus_tag="OQM83_003933"
     CDS             complement(823..2154)
                     /gene="xylB"
                     /locus_tag="OQM83_003933"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCS1522048.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVDSQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESVEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQKLDYRTGGGCGASTGRSKAGADCGESREIAH"
     gene            complement(2226..>2903)
                     /locus_tag="OQM83_003934"
     CDS             complement(2226..>2903)
                     /locus_tag="OQM83_003934"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="catalyzes the interconversion of D-xylose to
                     D-xylulose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCS1522049.1"
                     /translation="VVEHKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGL
                     EKEIKLNIEANHATLAGHSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEE
                     NALVMYEILKAGGFTTGGLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIE
                     DGELDKRIAQRYSGWNSELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLV
                     NHYLFDK"
BASE COUNT          767 a          794 c          635 g          707 t
ORIGIN      
        1 agtacggcaa aataataccc tttttcattt aactgcatct ctgcattcca tagccctaac
       61 agataggtaa cgataccacc aacgagagct atccatgaca caatactaaa ggccggtgaa
      121 taggttgata ttttgttgtc catcacagta ttcctttcat tcctgaatgt gtaagagctt
      181 tatgttgctc ataccgagcg aaacaaacgc atttgaccaa acaagcagac aaaaaaggcg
      241 atggggtaga tcaccatttc ataagcattg ataggtgcat ttaaaacacc gataaacaag
      301 agaccgatag tgattaacgc aactaactgg cagatatggg taaaaaaatt atccagttgc
      361 ccgagttttt ctgcgcgaag atatgcatag ttaccaaaag ttgctgtcat taacacgcca
      421 agaaaatagc cttttccact tagcaatggg catgagatcc atagcccaac cagataaacc
      481 agcgccccga caacaaataa tagttttgcc acagtttttg aggttttcat aacgatctcc
      541 atatctacca gcgatacatt acgagtaacc aacgaaagac aaaactgaag aatgccatta
      601 acaaatgatt ttcagaataa attcatacta aatattaatt aattagtgag atatatagat
      661 gtgaattatc ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt
      721 tgctgataga ggcgacggaa cgtttcacgt cgtggctgat aagcggcata acgctgcgca
      781 tctggtagat gcgactgttc taacggtagc tgcggcaaca actcaatgag cgatttctct
      841 ggattcgccg caatctgcgc cagccttgct gcgcccagtg ctggccccac atcccccccc
      901 cgtacggtaa tcgagcttct gaccgctgat atccgccagc atctgacgcc agtactcact
      961 acgcgccccg ccaccaatca acgtaacact ttgcggttta atgccgcagg catgcacgac
     1021 atccatgcca tctgccagcg cataacccac gccttccagc actgctcgcg ccagttcatt
     1081 ggggccatgt tgatgagtca aaccaaagaa aacccccttc gcctggggat tattgtgtgg
     1141 tgtacgctcg ccggaaagat aaggcagaaa ccaaactggc tcgacacttt catcagcctg
     1201 ttgagctgca gcgattaaag ctgggacatt gcacaggccg gttaatttcg cggcccaatc
     1261 cagacacgat gctgcactca gcattacaga cattaaatgc caacgctgcg gtagcgcatg
     1321 gcaaaagctg tgtacagcgc tttctggctt gcttaagaac ccttcgctga cagcaaaata
     1381 gacccccgac gtccccagcg ataacattgc ctgattagca tcaaccattc ccacaccaac
     1441 tgcaccagct gcattgtcgc caccgcctgc gacaactggc accgtcgcca taccccacgc
     1501 tttcgcaact tcaggtaaca aagcaccagt aatttcgctg ccttcgtata atgcgggcat
     1561 ctggtcacga gataagtcgc aagcctgcag catgacgtca ctccagtcac gctttgcgac
     1621 atccagccac atggtgccag ctgcgtcaga catatcgctg gcaaactccc ccgtcatacg
     1681 cagacgcaag taatctttcg gtaataatac tttgtcgatt tgacggaata tctccggctc
     1741 atgccgctga acccatagca atttaggcgc agtaaatccg ggcatcatca ggttgccggt
     1801 aatcactcgt gattgcggaa ctctcgcttc cagcaaagtg cactcttgcg cacagcgccc
     1861 gtcgttccac aaaatggcag ggcgcaatac ccgttgttga gcatccagta aggttgctcc
     1921 atgcatctgg ccggcaatac ccaatgcttt aacgtcctgc agagaatgct gatcgcccag
     1981 agctttcatt gcgcgatcag ttgcctgcca ccactgttcc gggtcttgtt ccgaccagag
     2041 tggatgcggg cgcgaaacgg tcagcttttc cgtttgcgaa tcaaccacct caccctgctc
     2101 gttgagcaaa ataactttta cgcccgaggt gccaagatct atcccgatat acatatcgat
     2161 cgttccttaa aaaaatgccc ggtatcgcta ccgataaccg ggccaacgga ctgcacagtt
     2221 agccgttatt tgtcgaacag ataatgattt accagatttt ccagttgctc ctggcgacca
     2281 ctctgatgca ccggagacaa attatgttcc tgagcatatt tggctaaatc tgccagtgac
     2341 atttggcctt tcaggatttg ctggcccaat tcgctattcc agccggaata acgctgcgcg
     2401 atgcgtttat ccagctcgcc atcttcaatc atgcgcgctg caattttcag cgccagtgcc
     2461 atcgtatcca tcgcgccgat atgaccgtaa aacagatcat atttatcagt actttgacga
     2521 cgtactttgg catcgaagtt cagaccaccg gtggtgaaac cgcctgcttt gagaatttca
     2581 tacatcacca gcgcattctc ttccacactg ttcgggaact ggtcggtgtc ccagcccagt
     2641 tgcgcatcgc cacggttggc gtcgacagaa ccgaacaggc caagcgcaat ggcggtggct
     2701 atttcatgat ggaaagagtg acctgccagc gtcgcgtggt tagcttcaat gttcagttta
     2761 atctcttttt ccagaccaaa ctgtttcagg aagccataga ccgtcgcggc atcgtaatca
     2821 tattgatgtt tagtcggttc ttgcggtttc ggttcgataa gcaacgtgcc ctggaagccg
     2881 attttatgtt tatgctcaac cac
//
LOCUS       DAJVQG010000252         2900 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJVQG010000252 DAJVQG010000000
VERSION     DAJVQG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.258"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..528
                     /locus_tag="OQM83_003935"
     CDS             <1..528
                     /locus_tag="OQM83_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS1522050.1"
                     /translation="ANYQVQNDGQRYALSQAKSIADELMTGCTNFAFSGKPGTGKNHL
                     AAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRELCEVDLLVLDEIGIQ
                     RETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERVMDRMVMNGGRWVNFN
                     WESWRPNVSHSRVVK"
     gene            543..965
                     /locus_tag="OQM83_003936"
     CDS             543..965
                     /locus_tag="OQM83_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCS1522051.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            1023..1379
                     /locus_tag="OQM83_003937"
     CDS             1023..1379
                     /locus_tag="OQM83_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522052.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            1472..1690
                     /locus_tag="OQM83_003938"
     CDS             1472..1690
                     /locus_tag="OQM83_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCS1522053.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            1692..2057
                     /locus_tag="OQM83_003939"
     CDS             1692..2057
                     /locus_tag="OQM83_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1522054.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            2054..2719
                     /locus_tag="OQM83_003940"
     CDS             2054..2719
                     /locus_tag="OQM83_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522055.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVECWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            2719..>2900
                     /locus_tag="OQM83_003941"
     CDS             2719..>2900
                     /locus_tag="OQM83_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCS1522056.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVAPAGWISCSDAVPA"
BASE COUNT          804 a          595 c          808 g          693 t
ORIGIN      
        1 gcgaattacc aggtgcagaa cgacggccag cgatacgcgt taagccaggc gaaatccatc
       61 gccgatgaac tgatgaccgg gtgtacaaat tttgcgttca gcggaaaacc tggtaccggg
      121 aagaatcact tagcggcagc tatcgggaat cgcctgctga aagacggtca gacagtgatt
      181 gtggttaccg tggctgatgt tatgagcgcc ctgcacgcca gctatgacga cgggcagtca
      241 ggcgaaaaat ttttgcggga gctgtgcgaa gtggatctgc tggttcttga tgaaattggc
      301 attcagcgcg agacgaaaaa cgagcaggtg gtgctgcacc agattgttga tcgccggaca
      361 gcgtcgatgc gcagcgtggg gatgctgaca aacctgaact atgaggtgat gaaaacattg
      421 ctcggcgagc gggtgatgga tcgcatggtc atgaacggcg ggcgctgggt gaattttaac
      481 tgggagagct ggcgtccgaa tgttagccat tcgagggttg ttaagtagtt tcaggaggat
      541 ttatggcgaa accttttact cccgaacagc gggaagaact gaagacgcga attgtggaac
      601 tcgtgcatca ggacggtcgg gtcacgattc ggcagttgtc agatgaaaca ggtatcagtc
      661 gtgcgtctgt cggtcgctta tgcatagaac tggtcgcaag tggtgatgta tataattctg
      721 gctacggctt attcccgtct gaacaggctc gcaaggactg gcaaagcgcc cgcaaaaaac
      781 tctcgagagt aaaggtgagg aaaccggttg ttgttgatcc ggaccttatc tggtcattac
      841 ctgacggaga aatacgccgc tacgacaggc gcctgaatat aatctgtcgc gagtgccgga
      901 agagcgaagc tatgcagcgt gtactggctt tctatcaggg taattttcag gaggcgatac
      961 tgtgaatgaa attagctatc aggcttcaat taccgctggc attcgcatca aaggagagga
     1021 gcatggaaat aaaaccagaa gatgagttaa gcaatatcgt tttatttccg gtaaaagagg
     1081 atgaccctcg taatcaggtt aattttcttt atgagccatc ggaaagacca tattgccatc
     1141 acgcctctgt acgggttgac gaaaaagagc gtcaggtccg ctgtaaaatc tgcggtgcgg
     1201 ttgtggagcc gtttgactgg atgctctctg tggcaaaaag agaaaccaga ctggcagatg
     1261 atgtaaggct attgcgccag gaggaacagg aaaggcggag aaatatagaa aagctgatac
     1321 agattgagcg taacgcgaaa gcgcggatac gcagggcgac aaaatccaga actgaataat
     1381 taaatttagc actgttaaaa atttaatcct taaccggagg gatttctgca ccctcaaatc
     1441 atcaggagac cgcccgaaag ggcggtaatg aatggttaca ttatttagaa aaaaatatcc
     1501 gcgaaagagt agaacaacag aattcctgtt tctcattctg tttatcgtgt tgatgacacc
     1561 gatatccccg ctaatttttg tctggataat cggaaaaata attgagccag ttattgaatt
     1621 gtataacgac gtggtatggg cgtcattcaa cacactgcac aataaaatta atccgtataa
     1681 ggaaagctga tatggcactg acgaaaaaac aacgtgcaga actgcgcatg aagttcggtg
     1741 gtcgctgcgc ttattgcggc tgcgaacttg gcgaaaagtg gcatgcagac catgtaaaac
     1801 cggtcattcg ttttgatgga aatatgcttc accaggaacg tgacgatata tccaacatgg
     1861 taccagcatg ccacccatgc aatctgcaca agcattgcag tagcctggaa gattatcgac
     1921 gaattatcag tgatggtcgt cgtgaattcc ttgcgtccgg gaaaggcaaa gcgctggttc
     1981 gtatgggatt ggttgaaatg aaatctgacc cggttgtgtt ctggtttgaa aaatatcaag
     2041 aaggggctac ggcatgacca ctattaccaa agagcgactg caatggctgg ctaacatttc
     2101 tggccgcgat gatattgacg atatagacgg cggtgaaatt cgtgaactgg cgcttatcgc
     2161 tctggcatca ctggaagcag agcctgtagc ggaatgcatt gttgaagatg ggggcatgtg
     2221 tattgacggg ttcggtgagt atgtgggtca ctcgctgcct gatggaatgc atgagcttta
     2281 tgctgccccg cgaatgcgac aacctgatgg atggaaagcc gtagctgtag cgtggaaggt
     2341 gacgtttact caagttgacc aggaatctaa tacgttcact gctatatatt ttgacaaagc
     2401 ggaagttgag tgttgggtac gactgcataa agcatgtgat tttcgggcag atataacacc
     2461 gctttacgca gcgccggcag tgccggttgc agtaaacgac gacatggctt acgcattcca
     2521 tcacgcactg tcagattcat cgcttggctc tgatgaaatc gaagaaatta aaaccggttt
     2581 gcgtgctgcc tttgccaacg tcactatcca accagagccg atagtgccgg atgaaatcgg
     2641 gccaaacgat agtaatacgt ttgattatgt tgatggctgg aacgcctgcc gcgctgccat
     2701 gcttaaggga gataaataat gattaatcga accaaactgg agcacattct cgaatatgcc
     2761 aggcagcaga aatgtattgg gcaactttgt aaaattccac caggagatat ggttgaaatc
     2821 gtggaaatgg ccatgcgtaa ggctggcaac tctccggtag ctccggctgg ctggataagc
     2881 tgtagtgatg cagttcctgc
//
LOCUS       DAJVQG010000253         2885 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJVQG010000253 DAJVQG010000000
VERSION     DAJVQG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.259"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..328
                     /locus_tag="OQM83_003942"
     CDS             <1..328
                     /locus_tag="OQM83_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCS1522057.1"
                     /translation="IQKIVGDKGLVARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVEL
                     QPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMR
                     YGEEVV"
     gene            555..2003
                     /gene="uxaB"
                     /locus_tag="OQM83_003943"
     CDS             555..2003
                     /gene="uxaB"
                     /locus_tag="OQM83_003943"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCS1522058.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(2175..2483)
                     /locus_tag="OQM83_003944"
     CDS             complement(2175..2483)
                     /locus_tag="OQM83_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1522059.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLMRKVVSAMISWIILSRARQKYGAASVVVDEQSWLSSGVKKSPY
                     "
     gene            complement(2477..>2885)
                     /locus_tag="OQM83_003945"
     CDS             complement(2477..>2885)
                     /locus_tag="OQM83_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCS1522060.1"
                     /translation="AKDNATLERYRRALNIKFNQPEQAARTLSGGNQQKILIAKCLEA
                     SPQALIVDEPTRGVDVSARNDIYQLLRSIAAQNVAVLLISSDLEEIELMADRVYVMHQ
                     GEITHSALTGRDINVETIMRVAFGDSQRQEASC"
BASE COUNT          709 a          704 c          739 g          733 t
ORIGIN      
        1 tattcagaag attgtcggtg ataaggggct ggtggcgcgc atgggcggcg aagaatttgc
       61 tgttgcagtg ccgtcggtga atcctgtcga tggtctgcta atggcggaaa aaatccgtaa
      121 aggcgttgaa ctgcaaccat tcacctggca acaaaaaacg ctttatctca cggtaagtat
      181 tggcgtcggt agtggtcgcg catcgtaccg aacgctgacc gatgatttta ataaattgat
      241 ggtcgaagcc gatacgtgtc tgtatcgctc gaagaaagat gggcgcaacc gtaccagcac
      301 tatgcgttac ggtgaggaag ttgtctgaca tctttattca ttccgcgcga tttgaatgca
      361 atgtgttcta ccactttatg atccaggaaa aggcgtattt cctcgattaa accatgatcc
      421 gcgccacact tttctcataa aactgctcat taatcatcag gagttgatca aaacaggggt
      481 tcggcgtatt aaagtttgct aacctgtcgt accaattcaa acaggttgta tgactaatca
      541 gaagggaacc cattgtgaaa acactaaatc gtcgcgattt tcccggtgca cagtatccag
      601 aacgtatcat tcagtttggt gaaggtaact tcctgcgcgc ctttgttgac tggcaaatcg
      661 atctcctgaa tgagcacacc gatctgaatt ctggcgtggt cgttgttcgt ccgattgaaa
      721 cttcattccc gccgtcactg agcacgcagg atggtctgta taccaccatt atccgtggcc
      781 tgaatgagaa gggggaagcg gtcagcgacg cgcgtctgat tcgctcggtt aatcgtgaaa
      841 tcagcgtcta cagtgaatac gatgaattcc tgaaactggc gcacaacccg gaaatgcgct
      901 ttgttttctc taacaccacc gaagcgggga tcagctacca tgcgggcgat aaattcgatg
      961 acgcgccagc gataagctat ccggcaaaac tgacccgtct gctgttcgaa cgctttagcc
     1021 atttcaacgg tgcgctggat aaaggttgga tcatcatccc gtgtgagttg atcgactaca
     1081 atggcgacgc gttgcgtgaa ctggtgctgc gctatgcgca agagtgggca ctgccagaag
     1141 catttattca atggctggat caggctaaca gcttctgttc tacgctggtg gaccgtatcg
     1201 ttaccggtta tccgcgcgat gaagtggcta aactggaaga agaactgggt tatcacgatg
     1261 gtttcctcga caccgctgaa cacttttacc tgtttgtgat tcaggggccg aaatccttag
     1321 cgactgagct gcgcctggac aaatatccgc tcaacgtgct gattgttgac gatattaaac
     1381 cgtataaaga acgcaaagtg gcaatcctca acggtgcgca caccgcgctg gtgccggtgg
     1441 cgttccaggc agggctggat accgtaggtg aagcgatgaa cgatgctgaa atttgcgcat
     1501 tcgttgaaaa agccatctac gaagaaatta ttccggtact ggatttgcct cgtgatgaac
     1561 tggaatcttt cgccagtgca gtcaccgggc gtttccgtaa cccgtataag catcagctgc
     1621 tgtctatcgc gctcaacggt atgaccaagt tccgcacccg catcctgccg cagctgctgg
     1681 cagggcagaa ggcaaacggc acacttccgg cgcgccttac tttcgcatta gcggcattga
     1741 ttgcgttcta tcgtggtgag cgtaacggag aaacatatcc ggtacaggac gatgcacact
     1801 ggctggaacg ttatcagcaa ctctggagcc agcatcgtga ccatgttatc ggtacccagg
     1861 aactggtagc gattgtgttg gcagaaaaag accactggga gcaggacctg acgcaagtgc
     1921 ctggtctggt cgagcaggtt gctaatgacc tggatgcgat tcttgaaaaa gggatgcgcg
     1981 aagctgtacg tccgttgtgc taataacacc gttacttctg aacccggttt tgaccgggtt
     2041 tcttatttca ctatattagt tacaacatcc tatgtgcctt tatcattaac agatgccaca
     2101 tgcagcgcct gttaatgttg taagcttgta ggtaatttgt aagtagcgtc ttgagaatgt
     2161 gatcgtaatc acgtttaata gggcgacttt ttcacgcctg aagataacca tgattgttcg
     2221 tccacaacaa cactggctgc gccgtatttt tgtctggcac ggctcagtat tatccaagat
     2281 atcatcgcgc ttactacttt gcgcataagc attaccagcg tcgcgcccat tgccagcagg
     2341 atcaggattt gcgcgctgct ataaaccatg gtcagcgttt gcacacttaa atactggcgg
     2401 tcgagaaaac cgggtaatac aaacagcagc accaccgcca gcagtgccgt gatttcacgg
     2461 ttgttctgaa taaacttcag catgacgcct cctgacgctg actatcgccg aaggcaacgc
     2521 gcataatagt ctcgacatta atatcgcgcc cggtcagtgc agagtgggta atttcgccct
     2581 gatgcatcac atacacacga tctgccatca gttcgatctc ttccagatca gaggagataa
     2641 gcagcactgc cacattctgt gcggcgatgc tgcgcaacag ctggtagata tcattacggg
     2701 ccgagacatc cacgccgcgc gtcggctcat cgacaatcaa tgcttgcggc gaagcttcca
     2761 ggcatttggc aatcaggatt ttttgctggt tgccaccgga taatgtccgt gcagcttgtt
     2821 ccggttggtt aaatttaata ttcagcgccc gacgataacg ttccagggtg gcattatctt
     2881 tcgcg
//
LOCUS       DAJVQG010000254         2874 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJVQG010000254 DAJVQG010000000
VERSION     DAJVQG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.260"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            257..412
                     /gene="hokD"
                     /locus_tag="OQM83_003946"
     CDS             257..412
                     /gene="hokD"
                     /locus_tag="OQM83_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCS1522061.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            716..988
                     /locus_tag="OQM83_003947"
     CDS             716..988
                     /locus_tag="OQM83_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522062.1"
                     /translation="MPSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEWLI
                     QCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            1062..2048
                     /locus_tag="OQM83_003948"
     CDS             1062..2048
                     /locus_tag="OQM83_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCS1522063.1"
                     /translation="MSAFHNGRILVEPEPKSMRNLPSGVVPAARQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSVYSESDIIPGEQTATSILKQRTKNIALLPHVHQQQNPPQEKTVVS
                     IAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTK
                     ADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            2049..2429
                     /locus_tag="OQM83_003949"
     CDS             2049..2429
                     /locus_tag="OQM83_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCS1522064.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            2426..>2874
                     /locus_tag="OQM83_003950"
     CDS             2426..>2874
                     /locus_tag="OQM83_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS1522065.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCT"
BASE COUNT          756 a          670 c          798 g          650 t
ORIGIN      
        1 atggcggtgg cgctggtggt gtattcgatt tgccgctggg gataaaaacg gtttgcggga
       61 aaaggagagt taagtagaat tgcagcgggt gcttgaggct atctgtctca ggcatgaaca
      121 ccaaaaggca gatagagaaa agccccagtt aacattacgc gtccggcaag acgcttaaca
      181 ttaatctgag gctcaatcta tgaacggcaa atctaggtta gcctcttacg tgccgaaagg
      241 caaggagaag caggctatga agcagcaaaa ggcgatgtta atcgccctga tcgtcatctg
      301 tttaaccgtc atagtgacgg cactggtaac gaggaaagac ctctgcgagg tacgaattcg
      361 aaccggccag acggaggtcg ctgtcttcac agcttacgaa cctgaggagt aagagaccag
      421 gcgggggaga aatccctcgc cacctctgat ggggcaggca tcctcaatgc acccacactt
      481 aacccgcttc ggcgggtttt tgtttttatt ttcaacgcgt ttgaagtttt agatggtgcc
      541 ggaatagaat caaaaatact taagtagcgc gcagggagaa gagggatgga ccccgaacag
      601 gggagtgcta tttatctgga aggattctgt tgatgaaaat cgaagaatta cgtgaaattt
      661 ttagtgaaga tggcctctat actgtgcgcg ttgagaatgg cgctattgtc agccactgcc
      721 ttccagacta cacaattctg ataattcagc cgtctttgcc agcaggcacg ggcggcgctc
      781 tcacgcattt aaatctgatt ggtaccagca tcccccatgc actgaagaac aggccgaatg
      841 gctcattcag tgttaccgca ggcgcggatg cgaggttaaa aaagccctta gcctcgacta
      901 ccgtcactgg ataatctccg tcaggctccc ttactccgaa cggccaacgc gtccgtcccg
      961 cacattccag caacggatct ggaggtaatg tgcgggtatt acttcgacct gttctggtac
     1021 cggaactcgg tctggttatc gttaagccag gccgtgaatc aatgtcagca ttccataacg
     1081 gcagaatact ggtggagccg gaaccaaaaa gcatgcgtaa tctgccgtcc ggggtcgttc
     1141 ctgccgctcg ccagccgctg gtggaagaca aaacattgct gccgtttttc agtaacgcac
     1201 gggtgattcg tgctgctggt ggtgctggtg cattgtctga ctggctgttg cgccatatta
     1261 aatcctgcca gtggccacac ggcgattatc atcacagcga aaccgtcatt caccgttatg
     1321 gtaccggcgc aatggtgttg tgctggcact gcgacaacca gctgcgtgac cagacatccg
     1381 aatcactcga gcaacttgct catcaaaacc tgtcagcatg gatgattgac gtcatcggtc
     1441 acgcaataag cggtacgcag gagcgtgaat tatctctggc tgaattatcc tggtgggcgg
     1501 tccgcaatca ggtggcggac gcgctaccgg aagcggtatt acgtcgttcg ctggggttgc
     1561 gtgcggaaaa aattcgctct gtgtacagtg aaagcgacat cataccggga gagcagacag
     1621 ccaccagcat actgaagcag cgcacaaaaa atattgcgtt actgcctcac gtccaccagc
     1681 aacagaaccc accacaggaa aagacggtgg tcagcattgc cgttgatccg gagtcaccgg
     1741 ctcagtatct ccagcgccag aaatcacaac gggaagagat gcctgtatac acgcgctggg
     1801 taaaaacgca gaaatgcatg acgtgtggca atcaggcaga tgatccgcat cacatcattg
     1861 gtcatggact gggagggatg ggaacaaagg ctgatgattt gtttgttatt ccgttatgcc
     1921 gtaaatgccg taacgaacta catgccggag taaaagattt tgaagagaaa cacggcagtc
     1981 agctgttgtt gctgattcgt tttttaatgc acgcgagaaa ttcgggtgtt ctgaagtgga
     2041 aagcataaat gaccgagcgc atagaatttg ttttacctta cccgccgacg gtgaacactt
     2101 actggcgtcg tcgtggcagc acatattttg tatcaaaagc cggggagcgt tatcgccggg
     2161 cagtggcgct tattgttcgc cagcagcggc tgaaattaag cctgtccgga aggttggcaa
     2221 taaaaattat tgcagaacca ccggataagc gccgccgtga cctggacaat attctgaaag
     2281 cgccgctgga tgcgctgacg catgcggggt tgctaatgga cgatgagcag tttgatgaaa
     2341 tcaatatcgt tcgtgctcag ccagtatctg gtggacgtct gggggtgaag atttatccca
     2401 taatgcttga agggcaggtc aaaaaatgaa actggaagat ttaccgaaat actactcccc
     2461 aaaatccccc ggcctgactg atgcatcggc ctcaacgtcg aaagatgcgc tgagtatcac
     2521 tgatgtgatg gccgcgcagg gcatgacaca gaatcgggct gagatggggg tttctgcgtt
     2581 ccttgggaaa atgggcatta gtatgaatga cagagagcgg gcaacagaat tgctgacaga
     2641 atatgcactc agtcggtgtg atcgcgtggc ggcgttaaga aaactcccgg cagaaataaa
     2701 accggcagtg atgcgtatta tggcttcgta tgcgtttgaa gattatgccc gtagcgcggc
     2761 gagcaaaaaa cagtgcccct gctgtcacgg aaaaaaattt attgaaagcg aggtttttac
     2821 aaacaagatc cagtatccgg atggtaagcc gccggtatgg gcaaagtgta cgaa
//
LOCUS       DAJVQG010000255         2865 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJVQG010000255 DAJVQG010000000
VERSION     DAJVQG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.261"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            1..1050
                     /locus_tag="OQM83_003951"
                     /pseudo
     CDS             1..1050
                     /locus_tag="OQM83_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            1066..1815
                     /locus_tag="OQM83_003952"
     CDS             1066..1815
                     /locus_tag="OQM83_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS1522066.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            1817..>2865
                     /locus_tag="OQM83_003953"
     CDS             1817..>2865
                     /locus_tag="OQM83_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522067.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVIL"
BASE COUNT          905 a          570 c          595 g          795 t
ORIGIN      
        1 gaattatcag aatgaaaaag agagttatat tgtcacgttt aaccagtatc ttgaatcatt
       61 aaggttaaat acatacgtaa gtttggctcg taatacttac tgggacgcca gcagtaatgt
      121 gaattattca ttatcactta gccgcgattt tgatatcggg ccattaaaaa acgtctccgc
      181 ttctctaaca tttagccgaa taaactggga agaagacaac caggatcaac tgtacctaaa
      241 tatttcgatt ccctggggaa ccagtagaac attgagctat ggtatgcaac gaaatcagga
      301 taacaagatt tcgcatactg cttcgtggta tgactcttcc gatcgaaata attcctggag
      361 cgtttctgct tcaggcgaca atgatgaatt caaagatatg aaagcgtcac tacgcgccag
      421 ttatcagcat aataccgaga acggtcgtct ctatctctcc gggacatcac agcgagacag
      481 ttattattct ctgaatgcca gttggaatgg ttcattcacg gcgactcgtc acggagccgc
      541 tttccatgac tatagtggta gtgctgactc gcgttttatg atcgacgcag atggtgcaga
      601 agatattccg ttgaacaata aacgcgcggt aactaatcgg tatggcatcg gagttattcc
      661 atcagtcagc agttacataa caacatcatt aagcgttgat acccgaaatc ttccagaaaa
      721 tgtggatatc gaaaactcgg ttatcaccac caccttaacc gaaggtgcta ttggctacgc
      781 caaacttgat accccaaggg ctatcaaatc atgggggtta ttcgcctggc agatggtagt
      841 cacccaccac tggggattag cgtaaaagat aaaaccagcc ataaagaatt aggactggtt
      901 gctgatggcg gctttgtata cctcaacggc attcaggatg acagcaaact tactttacgc
      961 tggggtgata aatcctgttt tattcaacca cccaatagca gcaacttaac caccggaacg
     1021 gttattttac cgtgtattag ccaaaattaa ttcaggatga aactaatgcg ctacttaaac
     1081 acaaagaatc tcattgcagc aggagtatta ctcgcctgca tgagcagcat tgcctggggt
     1141 gccatcatcc cggaccgtac ccgtatcata atgaatgagt cagataaagg ggaagcatta
     1201 aaacttacta atcaaagcaa aaatctaccc tatctggcgc aaacatggat agaagatacg
     1261 aaaggcaata aatcacgtga ctttattgtc acggttcccc ctatggtacg tttaaatccc
     1321 agtgagcaaa ttcaaataag aatgataagt caggaaaaaa ttgctcaact gcctaatgac
     1381 agagaaacgc tcttctattt taacgtgcga gaaataccgc caaaaacaga taaaaaaaat
     1441 gtgatgcaag tcactatgca gcacgcattg aaactattct ggcgaccaaa agctattgaa
     1501 ttagaagatg atggggtaat gtcttacgaa aaagttgaaa tcatccgtag aaatgatggt
     1561 tcgatccgct ttaacaataa gatgccttat cacgttacgc tgggttatat cggaacaaac
     1621 ggaatcacaa tgttgccaca aacgcaaagt ttaatggtaa ccccttatag ccatgcagat
     1681 acgcagttta aaaatgttcc gtcagctttt caggttggtt atatcaatga ttttggtgga
     1741 ttaagttttt atgaaataaa ctgcccgacg gttaataact cttgtaatgt ttccgtagcc
     1801 aaacgagata aataatatgc gctacttatt aattgtaatt atatttatta taggttttag
     1861 tgcattacct gtatgggcaa tggactgcta tgctgaacat gaagggggaa ataccgtagt
     1921 cattggttac gtaccaagaa tttctatccc cagtaatggt aaaaaaggtg ataagatctg
     1981 gcaaagcagt gagtatttta tgaatgtctt ctgtaataat gcattacctg ccccatcacc
     2041 tggagaagaa tacccatcag cctggactaa tataatgatg tttttaccag gcggtcagga
     2101 cttttataat cagaactcct atatttttgg cgtaacatac aatggagttg attatgattc
     2161 gactgcccct aatgctatcg cagcaccaga atgcattgat ataaaaggag cgggtacctt
     2221 taataatcac tataaaaacc ccgctgtctg tagtggaggt cctgagcctc aattatcagt
     2281 aacctttcca gctcgcgtac aactctatat taaattagca aaaaatgcca atagagttaa
     2341 taaaaacctg gtgttacctg atgaatatat cgctctagag tttaaaggta tgagtggcgc
     2401 aggagctata gaagtagaca aaaatttgac cttcaggatt cgtggattaa ataatattca
     2461 tgtccttgac tgctttgtta atgtcgcact ggaaccagct gatggcgttg ttgactttgg
     2521 taaaataaat tcccgaacaa ttaaaaatac cagcgttagt gagacgttta gtgtagtcat
     2581 gacaaaagat cctggtgcgg cctgtactga gcaattcaat attttaggga gctttttcac
     2641 tacggatatt ttgagtgatt atagccatct ggatatgggt aatggactgc tattgaagat
     2701 atttcataaa gatggaacag caacggaatt taaccgcttc tcacaatttg cttctttttc
     2761 atcgtctagt gcaccttcgg tcaccgcacc attcagggca gaacttagtg cgaacccggc
     2821 agagactgtt gttgagggac cgtttagtaa agacgtaatc ctcaa
//
LOCUS       DAJVQG010000256         2797 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJVQG010000256 DAJVQG010000000
VERSION     DAJVQG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.262"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            1..617
                     /locus_tag="OQM83_003954"
                     /pseudo
     CDS             1..617
                     /locus_tag="OQM83_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
     gene            614..1093
                     /locus_tag="OQM83_003955"
     CDS             614..1093
                     /locus_tag="OQM83_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HCS1522068.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(1147..2097)
                     /gene="corA"
                     /locus_tag="OQM83_003956"
     CDS             complement(1147..2097)
                     /gene="corA"
                     /locus_tag="OQM83_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCS1522069.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            2467..>2797
                     /locus_tag="OQM83_003957"
     CDS             2467..>2797
                     /locus_tag="OQM83_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522070.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          843 a          620 c          590 g          744 t
ORIGIN      
        1 tccggaacaa aaatatcaca acgtgaatgc actgatattt tatttgcaga aatgaaagaa
       61 ctagcaaaaa tgttttcctt ttttggacaa tataaaacac ttatcgaaga tctgattgaa
      121 cattttagat atggaaatgg cagcaatttt catagccaac aattaaattt atcttttcat
      181 gaaaaaataa atatggttat aatagtccaa tacgaataat caaagaatgt atcgaaaatg
      241 gcattaattc aactccctcg acaggatacc aacctcttat cctgcaaagc attaaaacca
      301 aattactttc atcaagacta aataaattta atgacttcga ggacagtttt aatggcttag
      361 gaatcagtgt tcatgatatt tcagctcaaa aaatatctct tttaagtttt caaaaatacg
      421 ctataggatg gagtgcaaca attcattttg tagcacagga ccacttcggt cttgatgtaa
      481 ctgatatcaa aaacaagact tacagcaaat atagattttt tcgcatatgg ttctttttac
      541 aacggcataa agactttgct ttcaaacctt ttttcaccaa ttttaacact atcgaaagaa
      601 tcgaaaatta tctatgaaaa gcaaactcca gaaaattata ttatgtttat ttcttctatg
      661 ctgcatctat aatttatgga cattgcgtcc tgtacagatt ttgtatacat actctgatgc
      721 tggcaattca gtctttctgg tcgttgatca tctgccatgg actgacagcg ataaaataaa
      781 ttggtatctg aagcatcaga atgaaattaa aaatcaacat cccctaccag aaggtagttg
      841 gcatacctgg tatgtcattg atatcggtaa tggcttcact gactataaaa aatatatcga
      901 agggccgtat gaagatctct actgtttccc gacaattaag agcaatgata attgcatcgt
      961 gaaaaattac ttaatggtta ttaatgaata cccttatcgt aacactcata ttggtattaa
     1021 cgattttact gagtatcaat taacacaaga aaataaaata gagcgagttt tcaatccgca
     1081 tgactttaaa taagacaatt tttagaaata agccggataa aaaaatagct ttatccggca
     1141 ctcatattac agccagttct tgcgcttaaa gtacagatac ggtgccaggc ccgcgaggat
     1201 cataaagata atcgcgcctg ggtagccgaa actccacttc agttctggca taaactcaaa
     1261 gttcatccca tagctggaag caacgagcgt cggcggcagg aataccacgg ataccaccga
     1321 gaagattttg atgatgcggt tctgctcgat gttgataaaa cccatcgccg cctgcatcag
     1381 gaagttcacc ttctggaaca gggattcgtt atgcggcagc agggattcga tatctcgcag
     1441 gatttcacgc gcctgctcca gttgcccacc cggtaaacgc gctttacgca ccaggaagtt
     1501 gagcgcgcgc tgggtatcca tcagacacag gcgaactttc cagccgatat cttccagttc
     1561 cgccagagtg gagagcgcct cgtcgtactc atcgccctga tgcccttcca taatcacccg
     1621 gctcaactgc tccaggtcgc tatagatatt ttcaatttca tctgccaact gttcgatttt
     1681 ggtttcgaac agatccagca gcagctcgta ggcgttaccg tctaccatcg actggctacg
     1741 ggcacgcata cgatacagac gaaaagcggg cagttcacgc tcacgcagag taaacagacg
     1801 accatcacgg atggtaaatg ccacagtgga gttaccggcg tgatcttccg catcttcaaa
     1861 gaagaagaag gagtgaatat gcaggccgtc gtcgtcttca aagaaacgtg ccgatgcttc
     1921 gatgtcttcc agttccgggc gggttgccag gctctggcca agttcagatt gtacgcgcag
     1981 tcgctcgtcg tcgtccggtt cgacaagatc aatccatact gcatttacaa ggggttgtga
     2041 ctcttcgact tccagccggg tcagtcggtt attttccagt tgaaatgcgc tcagcatgac
     2101 cgggactccc aatgcttaaa atatcggaca gttcggtggg cacacagaaa caaattgggt
     2161 ttcagaccat taaacagcct gactcagcgc gacgggaaaa attgaggtcg ctgacaaccg
     2221 ctaaggctat cagcaaaaag ggatagcctt aggagttgat cctggatgac aggatagtga
     2281 gccagtatct actgggtgtg tccaaggcga atgtcctctt agagtgatcg tgcgcgcatg
     2341 ttacgccagc aaaattttgc cgtcaacacg caacagaaca ccacagaaca atagttgaac
     2401 ttcgcgcttt ttggtactcg tcaaggtcgc taacaggata aaaaaatcca acatccagga
     2461 cctataatgg cgcatcggct actcattggt aaaggaatga tcaccttgaa tctcaaacgg
     2521 atttttctcg ccctcacctt acttccctta tttgctgttg ccgctgatga ttgcgcactc
     2581 tcagatccga tgctgaccgt acaggcgtat accgttaatc ctcaaacaga gcgggtgaaa
     2641 atgtactggc aaaaagccaa tggcgaagcg tggggaactt tacatgctct gctggtggat
     2701 atgaatagtc agggtcaggt gcagatggcg atgaacggcg gcatctatga tgaaagctat
     2761 gcgccgctcg gtttgtacat cgaaaacggt cagcaga
//
LOCUS       DAJVQG010000257         2788 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJVQG010000257 DAJVQG010000000
VERSION     DAJVQG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.263"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            44..472
                     /gene="uspC"
                     /locus_tag="OQM83_003958"
     CDS             44..472
                     /gene="uspC"
                     /locus_tag="OQM83_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCS1522071.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKFIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(479..1903)
                     /gene="otsA"
                     /locus_tag="OQM83_003959"
     CDS             complement(479..1903)
                     /gene="otsA"
                     /locus_tag="OQM83_003959"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HCS1522072.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(1878..2678)
                     /gene="otsB"
                     /locus_tag="OQM83_003960"
     CDS             complement(1878..2678)
                     /gene="otsB"
                     /locus_tag="OQM83_003960"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCS1522073.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
BASE COUNT          685 a          706 c          661 g          736 t
ORIGIN      
        1 aagctttaat gtcacctgaa gttcacagaa taaggaacag gttatgagct atagcaatat
       61 tcttgtcgct gttgcggtta caccggaaag tcagcaactg ctggcaaaag cggtatctat
      121 cgccaggcca gtaaagggac acatcagttt aattactctc gcttccgacc cggaaatgta
      181 caatcaatta gccgcgccga tgcttgaaga tttacgcagc gtgatgcaag aagaaacgca
      241 aagctttctt gataagttca ttcaggatgc ggggtatccc gttgacaaga cgtttattgc
      301 ctacggtgag ttaagcgaac atattctgga agtatgtcac aaacatcatt tcgatttggt
      361 gatttgcggt aatcacaatc acagtttctt ttcgcgagca tcctgctcgg cgaaaagagt
      421 gattgcctca agtgaggtcg atgtgctgtt agttccactt acgggagatt aaccgctcct
      481 acgcaagctt tggaaaggta gcaactttat cgcgctgctg gctttccgcg cttcgcggaa
      541 ctatctgctt taggtcgcta atgaagcact cctgccagtg gttaatatcg tttttcacga
      601 taacgtccag catttctgca tgacgggaaa tacgttccgc cagcgacata gtcaatgcac
      661 gatccagcgc agctgcaact tcgtcacgat cgtaggggtt aacaattaac gccgacgtta
      721 actcgtttgc cgctcccgca aattgcgaaa gaacaagaac gcccgggttg gctgggtcct
      781 gagcagcaac atactctttt gctaccaggt tcatcccgtc acgcagtggc gtcactaagc
      841 ccacgtcaga gtagcggaat attttcatca gtaatttacg gtcaaaatgc tgattcaaat
      901 aataaagcgg cgtccagcct aattgcccgt atttaccatt aattcgtcca gcttcatttt
      961 cgagctgatg acgaatatcc tgataggctt gcacatcacc acgcgacgtt ggtgcaatct
     1021 gggtataacg aattttacca tgatgctgcg gatatttttc cagcaacgct tcataggcga
     1081 gaaaacgctc tggcaaacct ttggaataat ccagccgttc gacagaaaag atattttgta
     1141 cgtttttcag ttccgcttta agttgcgcca gttttggcgg cagtggcccg gcagcctgtt
     1201 tggctatttc tttcggttca atgccgatcg ggtagacttc tgttcgaaat gctttgcccc
     1261 aggctgtatg gctttttgcg ctacgtgtcg tgacgcgggt caggttagaa agacaatcca
     1321 ggaacgccag acgatcgttt tctgtctgga aacccagcaa atcataatca caaagctgtt
     1381 caagcaaggt gtcatatgtc ggcagcgcgt tgaagatttc cggtgtcggg aaaggaatat
     1441 gcagaaagaa accaatgcga ttattcactc cccgtttgcg taattcatgc gcaaatggca
     1501 acaggtgata atcgtggatc cagataatgt catcgtcttg caacagcggc agtaatttat
     1561 ctgccagcaa cgcatttacg cgtagatagc cgtcccaggc aggacgctga aattgcacca
     1621 gatcgagccg ataatgaaaa gcgggccaga gaacggcatt ggagaattgg ttgtagtatt
     1681 cgtcaaggtc ctgttcgctg aggttaaaag aggcccacgt aatgttacct tttttcacct
     1741 tttttagcgg ctgatcctca ttccctgttt caccactcca gccaaaccac agtccgcctg
     1801 cggctttcag tgcccccagt atgccaacgg caaggccacc ggcactggcg gcgtgctcgt
     1861 ctggtggtgc aatccggtta gatactacga ctaaacgact catagtcatc actcctgtta
     1921 ttttctcttt tttgttgtaa tgcggtggtt atcatttcaa gccagctcca gacatccggc
     1981 acacccgcca gtcgccatga tgcctgagtt gcacctgtgc caatttttac tgacattccg
     2041 cccagtcggt taacgactgc gaagccagat tcatcggtta aatcatcgcc cagaaatacg
     2101 ggcgttcgcc cgataaaggg agcttcctgc ataaaagctg caattgcctc acctttactg
     2161 gtacctctcg gtttgatctc gacaacacac tttccctgct gtaacgccat ttgtggccag
     2221 atctgagtaa tacgttgcgc taatgtcatt aatgcgtctt catgctgcgg agcctgacga
     2281 taatgcagcg caaaagccat ccctttcgcc tccagctccg cgccgggata ctgagcgatg
     2341 actgtatgca gttgcacgct aatatcacgc gcaatcgcat ccggcagatg aacgatatgt
     2401 gttttaccat tgatgtcacg gcgctccgcc ccatgcacgc ccgctaacgg gaagcgataa
     2461 ggttttgcca gtgcgtcaag ctccaccatt gagcgccctg atatcaatgc caatgcacca
     2521 tcacttgcgg ttgccagtag ctgtagtcct tgcagaatat tgtcaggcac gacgacctga
     2581 tcgggatgcg gtttgatttc cgccagcgtt ccatcaagat caaaaaacca ggcatatttc
     2641 gcggatagtt caggggtttc ggttaacggt tctgtcaccc ggctctcctc cttctctttt
     2701 cttattgttt tgcgcagaac gcgctttaca gacatgtaag tatagacagt gtgacggggg
     2761 tcgccatttg caatgaagaa cagccaac
//
LOCUS       DAJVQG010000258         2780 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJVQG010000258 DAJVQG010000000
VERSION     DAJVQG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.264"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..183
                     /locus_tag="OQM83_003961"
     CDS             <1..183
                     /locus_tag="OQM83_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCS1522074.1"
                     /translation="PFVPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAW
                     YLVGLPIGPGIYPRLS"
     gene            194..709
                     /gene="ogt"
                     /locus_tag="OQM83_003962"
     CDS             194..709
                     /gene="ogt"
                     /locus_tag="OQM83_003962"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCS1522075.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            904..1656
                     /gene="fnr"
                     /locus_tag="OQM83_003963"
     CDS             904..1656
                     /gene="fnr"
                     /locus_tag="OQM83_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCS1522076.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            1808..2758
                     /gene="uspE"
                     /locus_tag="OQM83_003964"
     CDS             1808..2758
                     /gene="uspE"
                     /locus_tag="OQM83_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCS1522077.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
BASE COUNT          712 a          661 c          694 g          713 t
ORIGIN      
        1 ccttttgttc cactgtttct tggattcctg caacgctaca aaccagacgc gaaactgggt
       61 acttactatt cgttagtctt gccctatccg cttatctttt tggtggtatg gctgctgatg
      121 ttgctggcgt ggtatcttgt tggtctgccg ataggtccgg ggatttaccc acgtttgtct
      181 taagagagaa cggatgctga gattacttga agaaaaaatt gccacgccac tgggtccact
      241 gtgggtgatt tgcgatgagc aatttcgcct gcgggcggtt gaatgggaag agtacagcga
      301 acgcatggtg cagctgctgg acatccatta tcgcaaagaa ggctatgagc gcatttctgc
      361 caccaaccca ggcggtttaa gcgacaagct tcgtgattat tttgccggta atcttagcat
      421 tattgatacg cttcccactg ccacaggggg gacgccattt cagcgcgaag tctggaaaac
      481 tctacgcact atcccctgcg gacaggtaat gcattacggc caactggctg aacaattggg
      541 ccgccctggc gcggcgcgtg ccgttggtgc ggcaaacgga tcgaatccca tcagcatcgt
      601 cgtaccttgc catcgggtta ttggccgaaa cggcaccatg accggatatg caggcggagt
      661 tcagcgaaaa gagtggttat tgcgccacga aggttatctt ttgctgtaaa cattaaacaa
      721 tttgtgccag cttgttcaca cttttatgta aagttaccct taacaactta agggttttca
      781 aatagataga catatattta catctaatat cggaattctc tgctgttaag gtttgcttag
      841 acttacttgc tccctaaaaa gatgttaaaa ttgacaaata tcaattacgg cttgagcaga
      901 cctatgatcc cggaaaagcg aattatacgg cgcattcagt ctggcggttg tgctatccat
      961 tgccaggatt gcagcatcag ccagctttgc atcccgttca cactcaacga acatgagctt
     1021 gatcagcttg ataatatcat tgagcggaag aagcctattc agaaaggcca gacgctgttt
     1081 aaggctggtg atgaacttaa atcgctttat gccatccgct ccggtacgat taaaagttat
     1141 accatcactg agcaaggcga cgagcaaatc actggtttcc atttagctgg cgacctggtg
     1201 ggatttgacg ccatcggcag cggccatcac ccgagcttcg ctcaggcgct ggaaacctcg
     1261 atggtatgtg aaatcccgtt cgaaacgctg gacgatttgt ccggtaaaat gccgaatctg
     1321 cgtcagcaga tgatgcgtct gatgagcggt gaaatcaaag gcgatcagga catgatcttg
     1381 ctgttgtcga agaaaaatgc cgaggaacgt ctggctgcat tcatctacaa cctgtcccgt
     1441 cgttttgccc aacgcggctt ctcccctcgt gaattccgcc tgacgatgac tcgtggcgat
     1501 atcggtaact atctgggcct gacggtagaa accatcagcc gtctgctagg tcgcttccag
     1561 aaaagcggta tgctggcagt caaaggtaaa tacatcacca tcgaaaataa cgatgcgctg
     1621 gcccagcttg ctggtcatac gcgtaatgtt gcctgatttt tccgcataac tcactatcct
     1681 tctgtcatat cattaaattt ttctgattta ttgatctggc agaaggttca tcactgtttc
     1741 attcaccaga tatgggttaa tcttttaatt acaaactgcg ttgacagttg ttgtaaggag
     1801 accctgtatg gctatgtatc agaacatgct cgttgttatc gatcctaacc aggacgacca
     1861 accagcattg cggcgagctg tttatttaca tcaacggatt ggtggcaaaa ttaaagcctt
     1921 tttgccgatc tatgacttct catacgaaat gaccaccctg ctctccccgg acgaacgtac
     1981 cgctatgcgt cagggcgtca tcagccagcg tacagcctgg atccacgagc aggcaaaata
     2041 ttatctcaat gctggcgttc ccattgaaat taaagtggtc tggcataacc gtcccttcga
     2101 agccatcatt caggaagtga tcagcggcgg gcacgatttg gtgctaaaaa tggcgcacca
     2161 acatgaccgt ctggaagcgg tgatttttac gccaacggac tggcatctgt tacgcaaatg
     2221 cccaagcccg gtgtggatgg tgaaagacca gccgtggccg gaaggaggta aggcgctggt
     2281 ggcggtgaat ctcgccagtg aagagccgta tcataatgcg ctcaatgaaa aactggtcaa
     2341 agagacgatc gaactggcag aacaagtcaa ccataccgaa gttcatctgg ttggcgctta
     2401 cccagtaacg ccaattaata tcgcgattga actgccggaa tttgatccga gcgtttataa
     2461 cgatgccatt cgtgggcaac atttgctggc aatgaaagct ctgcggcaga aattcggcat
     2521 taatgaaaac atgacgcacg tagaaaaagg tctgccggaa gaggttattc ctgatttggc
     2581 ggagcactta caggcgggga ttgtggttct gggcacggta ggacgcaccg gtatttcagc
     2641 agcattcctc ggcaacacgg cggaacaggt gattgatcat cttcgctgcg atctgttggt
     2701 cattaaacct gaccagtatc agacacccgt tgaactggat gacgaagaag acgattaacg
     2761 actcttcaat aactgacaag
//
LOCUS       DAJVQG010000259         2770 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJVQG010000259 DAJVQG010000000
VERSION     DAJVQG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.265"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(106..522)
                     /gene="rraB"
                     /locus_tag="OQM83_003965"
     CDS             complement(106..522)
                     /gene="rraB"
                     /locus_tag="OQM83_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCS1522078.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            684..1688
                     /gene="argF"
                     /locus_tag="OQM83_003966"
     CDS             684..1688
                     /gene="argF"
                     /locus_tag="OQM83_003966"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCS1522079.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(1760..>2770)
                     /locus_tag="OQM83_003967"
     CDS             complement(1760..>2770)
                     /locus_tag="OQM83_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1522080.1"
                     /translation="DLLEKPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEID
                     DLNAVNNYLLNNKKATDNEINDIKVNLSHILIDSLDDAKVNLTPVIDSILETFLKSPY
                     INDVRILDWCFNKRMQYFGDSEKIKYACSVINHIDFSCDQSKDFSCDQSKIKIAETLF
                     FNLDKEPYKNSRKLQELIWDKLVAYVNDFSLSNQEKSRLILRLFDDVKLLFNEVPVSI
                     LVNDIFLKDFFMKQPDFAKWYFYQLLKCYEGEQLYLNELGYVYGDEEKTKEIVNKLPG
                     YVVKIFEEKMGNELKIRTRMMKILRNGKINIYGYINEEQLGKLNPPEDLRIAIKKLGW
                     NN"
BASE COUNT          785 a          617 c          548 g          820 t
ORIGIN      
        1 tatgcgcttg gctgcacggg ccatgtcgat tgtgaagtgc ggatgctacg cgaactctaa
       61 aaaaatgccc gctagtatgt gctatcgggc attttaatca attaattagt ggcgaacccc
      121 gtcatcgtct tcatcgacaa aatcttcatc gtcgccatct tcgccgttag gatcttcaaa
      181 gtaagtgccc caaccgtcgt actcaacgtc aaatttctcc gccagcgtca tcagttgttc
      241 aacctgggca tcgatcagat cggcattcaa cgcgcactcg ctgaggatgt cgcagcaaat
      301 cacgatatca ccatcttcaa cttccagctc ttctggatcg gtcacttcgt aaccgagttt
      361 aaacgcttca actgccgctt tttccagagt ttccagatcg tctgcggaaa gatggtgttc
      421 gatggtgtac aacgcgtccg gatcgctgcc atcttccagt aattcttcaa taatcaaacg
      481 tgtttcttca cgctgttctt ccagttgttc cgggtttgcc atggctcatt cctcttaatg
      541 tcctgccgat actcttattg tcacacacca ctgacattgc ctccaccttt gtgataaaga
      601 tttatgcttt agacttgcaa atgaataatc atccatataa attgaatttt aattcattaa
      661 ggcgttagcc acaggaggga cctatgtccg ggttttatca taagcatttc ctgaaattac
      721 tcgatttcac gccagctgaa ctcaacagcc tgctgcagtt agccgcgaag ctgaaagccg
      781 ataagaaaag cggtaaagaa gaagccaaac tcactggtaa aaacatcgcg ctcatcttcg
      841 aaaaagactc gacccgtacc cgatgctctt tcgaagttgc cgcatatgac cagggggctc
      901 gtgttactta tctcggccca agcggcagcc aaattggtca taaagagtcg attaaagaca
      961 ctgcccgcgt gcttggtcgc atgtatgacg gtattcagta tcgcggctat ggtcaggaga
     1021 ttgtcgaaac actggcggaa tacgctggcg tgccggtatg gaacggcctg accaatgagt
     1081 ttcaccccac gcagctgctg gcggatctcc tcaccatgca ggagcatttg cccggcaaaa
     1141 cgttcaacga aatgacgcta gtctatgcag gtgacgcacg taacaacatg ggcaattcga
     1201 tgctcgaagc tgcagcgctt accggtctgg atttgcgtct ggtcgcgcca caggcatgct
     1261 ggccggaagc tgcgctggtt acggaatgcc gcgccctggc acaacaaaat ggcgggaata
     1321 ttacgctgac ggaagatgta gctaaaggag ttgaaggtgc tgactttatc tataccgatg
     1381 tatgggtgtc gatgggtgaa gcaaaagaga aatgggctga acgcattgca ttgctgcgtg
     1441 attatcaagt gaacagcaag atgatgcagt tgaccggtaa cccggaggtc aaattcctcc
     1501 actgcctgcc cgcgtttcat gacgaccaaa cgacgcttgg caaaaaaatg gcggaagagt
     1561 ttggcctaca tggcggaatg gaagtgactg atgaggtctt cgaatctgcc gccagcattg
     1621 tatttgatca ggcggaaaac cgcatgcata ccatcaaagc ggtgatggtc gcgacgctca
     1681 gtaaataatt gatatcgcta taaagatgcc gggaaataca aaaacatccc ggcgtcacat
     1741 tattttatgc agtgatagtt caattattcc accccaattt ttttattgct atacgtaaat
     1801 cttccggagg atttaatttt cctaactgtt cttcattaat atatccatat atattgattt
     1861 tgccattccg gaggattttc atcattcttg ttctaatttt taattcatta cccatctttt
     1921 cttcaaaaat cttaacgaca taacccggaa gtttatttac aatttctttt gttttttctt
     1981 catcaccata aacatatcct aactcattaa gatacagctg ttcgccctca taacacttaa
     2041 gtaattgata gaagtaccac ttagcaaaat caggttgctt catgaaaaaa tccttcaaaa
     2101 aaatatcatt tactaaaatg ctgacaggca cttcattaaa tagtaactta acatcatcaa
     2161 ataaccttag tattaagcgt gatttttctt gattgcttaa gctaaaatca ttgacatatg
     2221 caaccaactt atcccaaatc aattcctgta atttacggct atttttatag ggttctttat
     2281 cgagattgaa aaataaggtc tcagctattt taattttaga ttgatcgcag ctaaaatctt
     2341 tagattgatc gcagctaaaa tcaatatgat ttatcacgga gcatgcatac tttatctttt
     2401 cagaatcacc aaaatattgc attcttttat taaaacacca atccagtatt cttacatcat
     2461 taatatatgg ggattttaaa aaagtctcca gaatcgaatc gatgaccggt gtaaggttaa
     2521 cttttgcatc atctaaggaa tcaataagaa tatgagacag gttaactttg atgtcattaa
     2581 tttcattgtc agtagctttc ttattattaa gaagataatt atttactgcg ttaaggtcat
     2641 ctatttcttt cagtatccct ttttcaccaa gcaagtatga aatcgattca ttacatacat
     2701 aaggcattaa tagcagatgt tctttcctta ttgctaataa tgcattgttc aatggttttt
     2761 ctaataaatc
//
LOCUS       DAJVQG010000260         2721 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJVQG010000260 DAJVQG010000000
VERSION     DAJVQG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.266"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            30..2546
                     /locus_tag="OQM83_003968"
     CDS             30..2546
                     /locus_tag="OQM83_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1522081.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVIVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            2557..>2721
                     /locus_tag="OQM83_003969"
     CDS             2557..>2721
                     /locus_tag="OQM83_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1522082.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          778 a          657 c          630 g          656 t
ORIGIN      
        1 caaatataaa aaacacaggt catcagggaa tgccacaacg acaccaccag ggacataaac
       61 gcacaccgaa acagttggcg ctcatcatca aacgttgttt gccgatggtg ctcactggca
      121 gcggcatgct ttgcactacc gctaacgccg aagagtatta tttcgacccc attatgctgg
      181 aaactacaaa aagtggtatg caaacaaccg atctgtcacg tttttcaaaa aaatacgcac
      241 aactaccagg aacttatcag gttgatatct ggctgaataa aaagaaggtt tcacagaaaa
      301 aaattacatt taccgccaat gcagagcaac ttctgcagcc acagtttacg gtagaacaac
      361 tacgtgagct gggtattaag gtggatgaaa tcccggcgct ggctgaaaaa gatgacgata
      421 gcgtgatcaa ctcgcttgaa caaatcattc ccggtacagc agctgaattt gatttcaatc
      481 atcagcgact taatttgagc attccccaaa ttgcactgta ccgtgatgca agaggttacg
      541 tctccccttc tcgttgggac gatggtatac caacgctgtt taccaactac tcgtttacag
      601 gttctgataa ccgttaccgc cagggcaatc gtagccaacg acagtacctg aatatgcaaa
      661 atggtgctaa ttttggcccc tggcgattac gcaactattc cacatggaca cgcaacgatc
      721 agacatcaag ctggaatacc atcagtagtt atttacaacg tgatatcaag gcattgaagt
      781 ctcagttgct tctgggagaa agcgccacca gcggcagtat tttttccagc tacaccttta
      841 ctggcgttca actcgcttcc gacgataata tgttgccaaa cagccagcgc ggatttgccc
      901 caacggtacg cggtatcgca aacagtagtg caatcgtgac tatcaggcaa aatggttatg
      961 tgatctatca aagcaacgtg ccagcgggtg cctttgaaat taacgatctc tacccctctt
     1021 ccaacagcgg cgatttagaa gtcacgattg aagaaagtga cggtacgcaa cgtcgcttta
     1081 tccagcctta ttcttcatta cccatgatgc agcgacctgg gcatctaaaa tatagcgcga
     1141 ccgctggacg ctatcgcgct gatgcaaaca gtgatagcaa agaacccgaa tttgctgaag
     1201 ccacggcaat atatggtttg aataatactt ttacgctgta tggtggcctg ctcggttctg
     1261 aagattatta tgcgctgggg atcggtatcg gcggcacact tggcgcactg ggcgcgttgt
     1321 cgatggatat caacagagct gacacccaat tcgataacca gcactctttt catggctatc
     1381 aatggcgtac gcagtacatc aaagatatcc cggaaaccaa caccaatatc gctgtcagct
     1441 actatcgcta taccaacgat ggctatttta gttttgatga agccaatacc cgtaattgga
     1501 actataacag tcgccaaaaa agtgaaattc aattcaacat cagccagaca atatttgatg
     1561 gggtaagtct gtatgcctcc ggttcgcagc aagactattg gggcaataac gataaaaaca
     1621 ggaatatctc agttggggtt tccggtcagc aatggggaat tggttacagc ctgaattatc
     1681 aatacagtcg ctacacggat caaaataatg accgcgcact ctctttgaat ctcagtattc
     1741 cgttagaacg ctggttaccg cgtagccggg tttcctatca gatgaccagc cagaaagatc
     1801 gcccaaccca acatgaaatg cgtcttaatg gctcactgct ggatgatggt cgcctgagct
     1861 atagcctgga acaaagtctg gatgacgata acaaccataa cagtagcctg aacgccagtt
     1921 accgttcacc ttatggcacc ttcagtgccg gatacagcta cggtaatgac agcagccaat
     1981 acaattacgg cgttaccggc ggcgtggtta tccatactca tggcgtgacg ctctcgcaat
     2041 atctgggcaa cgcttttgcg cttatcgatg ctaatggggc ttctggcgtg aggatacaaa
     2101 actatccggg gattgctact gatccctttg gctatgcagt ggttccttat ctcacgactt
     2161 accaggaaaa ccgtctctcg gtagatacta cgcagctgcc cgataacgtc gatcttgagc
     2221 aaacaacaca gtttgtggtg cccaacagag gtgcaatggt agcggcgcgt ttcaacgcca
     2281 atatcggtta tcgcgtactt gttatagtca gcgatcgcaa cggtaaaccg ttgccctttg
     2341 gcgctcttgc cagcaacgat gagacggggc aacaaagtat cgtcgatgag ggcggcatac
     2401 tatatctctc tgggatatcg agtaaatcac aaagctggac tgtacgctgg ggaaatcagg
     2461 cagatcaaca atgtcagttt gcttttagta caccggattc agaaccaaca acctctgtat
     2521 tacaaggcac ggcgcagtgc cattaaggat aaaaaaatga aaagagcgcc tcttataaca
     2581 ggacttttgt tgatatccac atcctgcgct tatgcctcct caggagggtg tggagctgac
     2641 agcactagcg gtgcgacaaa ttacagcagt gtggttgatg atgttacggt gaaccagaca
     2701 gataacgtga caggacggga g
//
LOCUS       DAJVQG010000261         2690 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJVQG010000261 DAJVQG010000000
VERSION     DAJVQG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.267"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            230..769
                     /locus_tag="OQM83_003970"
     CDS             230..769
                     /locus_tag="OQM83_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCS1522083.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /locus_tag="OQM83_003971"
     CDS             complement(995..1318)
                     /locus_tag="OQM83_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCS1522084.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="OQM83_003972"
     CDS             1604..1798
                     /locus_tag="OQM83_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCS1522085.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcaggga caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttggtagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DAJVQG010000262         2690 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJVQG010000262 DAJVQG010000000
VERSION     DAJVQG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.268"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            533..2386
                     /locus_tag="OQM83_003973"
     CDS             533..2386
                     /locus_tag="OQM83_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCS1522086.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
     gene            2537..>2690
                     /locus_tag="OQM83_003974"
     CDS             2537..>2690
                     /locus_tag="OQM83_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCS1522087.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLT"
BASE COUNT          647 a          665 c          757 g          621 t
ORIGIN      
        1 cttccaggcc catccgctcc ggtgggcctt tttactgcag aaaacaggtt ccccgttaaa
       61 tgctatgttg ctcacaattc agtaagttga cagttgcctg tcagactggg catttgttaa
      121 aaaaatttcg catggtgaat ccccctgagc ggaggggcga ctggtgacgg tataatctct
      181 gattatcaaa acgagaatga cgcgggttta gtggcaccgg gctgaactca ccgggaggca
      241 cccggcacca tgtgcatgat gatacagata cgcggcttta gcccctctcc ggaggggttt
      301 tcttgtgggc aaaaaaagcc cgcgctggga gacgcgggcg gcaaggaata aacaataaaa
      361 cgtgaagtaa tatttcagct ggcgaataat accccatagt aatcactctg cgcaactgcg
      421 cggtcttttt cgaattgcgg gctgtcgtct ctcttctgcc attgtcctgt aacttccgga
      481 cttcagcccg ctccttattt tactcacaat attatcccgg ccgggaggat tcatggcatt
      541 taaacactat gacgtggtca gggcggcatc gccgtcagac cttgcgaaac gactgacaca
      601 aaaactgaag gagggctggc agccgtttgg tagtccggtg gccataaccc cttataccct
      661 gatgcaggcg attgcagcag aaggtgatgt ggtcgtcagt ggtgcaactg agccggagtg
      721 gtactacgtc atcgtactgg ccgggcaatc caatgccatg gcttacggtg aaggtcttcc
      781 gcttccggat tcatacgatg cgccccatcc gcgcattaag caactggccc gtcgtaacac
      841 agtgactccc ggtggtgaag tatgcgtatt taacgacatc attcctgctg accattgtct
      901 gcatgatgtt caggatatga gtacgattaa ccatccccgg gctgacctga gcaaagggca
      961 gtacggctgt gtcggacagg gcttacatat tgccaaaaaa ctgcttccgt atatccctaa
     1021 taatgcgggg atcctgctgg taccatgctg tcgtggtggt tcggcattca cccagggcac
     1081 ggaggggaca ttcagcgagt ccacgggggc cagtcaggat tcggctcgct ggggagtggg
     1141 taagccgtta tatcaggatc tgcttttccg cacgaaggca gcattgcaga aaaacccgaa
     1201 aaacgttttg ctggcgatat gctggatgca gggggaattc gatatgacga atgccagtta
     1261 cgcccagcag ccagcagcat ttcttgcaat ggtacagcag ttccgtgctg accttgccgg
     1321 gctggcggcg cagtgtcacg gtggaagtcc ggcatcagtc ccctggattt gtggcgacac
     1381 gacatacgcg tggaaacaag aacacggtac gcaatatgaa gtggtatatg gtgcatataa
     1441 aggtaaagaa tcccagcaga tttattttgt tccctttatg accgatggta gcggagttaa
     1501 tacaccgaca aacaacccgt cagaagatcc tgatattgcc gggtctggtt attacggttc
     1561 ggcatcccga acgaacaaaa actgggtatc atcaaatcgc ccgacgcatt tcagctcatg
     1621 ggcgcgtcgt ggcattattc ccgatcgtat ggcaactgct attctgaacg tagccggtcg
     1681 caccttagcc ttcattagtg gtaaggcacc ggaaatcaaa ccctcgcccg gcggcgacac
     1741 gccatcgggg ccgtctgaag atgcatccat acgcacaatc tccctgttgc cgacagccgg
     1801 agacgctgct gcgcagggct ggagcattaa gaatggcgga gttcagttgt cagatggtgt
     1861 atttaagatc accaagcaga gcaataaagc ctggtccctg acgcgcccgg tggatgacgc
     1921 agtctccctg ctgacacggg gtggcagact gagctgtaag tttcgactgt caggcgcact
     1981 gaccaacaac cagttcggtc tgggaattta tctgtatacc gatgtagcgt tacctgacgt
     2041 cgtggcgatg accgggactg gtaacccgtt cctgatgtcg ttcttcaccc agaccacaga
     2101 cggcaaactg aatctgatgc atcacaagaa agcaggaaac acaaagttgg gcgagttcgg
     2161 gaattacagt aacgactggc tgacgctgga gctggtgttc accgccggca gtgccacggt
     2221 tactccgaaa ctgaatggag tggctggccc ggcattccag gtcataaaag acagtctgac
     2281 actggggctg aatgcgctga cgctgacgga tattaccaaa aatgcagcgt atggcgttga
     2341 gatagaaagt ctggtgctgg agataaatgc accggcatca tcataaaaag tgagccagcc
     2401 aaatggaagg tatcgttaaa ctcaccggta gtgtcagtgg gtcgtctgag atgcctgcat
     2461 gagttatcag agccatcagt acttaactgg tggctttttt tattgttgtc agcttccgga
     2521 taacgggaga cggggtatgt accagatgga aaaaatcaca acaggtgtgt catacaccac
     2581 gtcagcggtg ggaacgggct actggttcct gcagttgctg gacagggttt ccccgtctca
     2641 gtgggcggca ataggcgtgc tggggagtct gctgtttggg ctgctgacat
//
LOCUS       DAJVQG010000263         2678 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJVQG010000263 DAJVQG010000000
VERSION     DAJVQG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.269"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..620
                     /locus_tag="OQM83_003975"
     CDS             <1..620
                     /locus_tag="OQM83_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HCS1522088.1"
                     /translation="MSGITLDPGLAPRAVWPTDSASVIRQKQQTNLVAAVIRTTAISE
                     ATRAVTSLPQPGSLVKNQQAVVAVGGSTERQSDIIHVSHPALDSVAASTGQDERAQPP
                     TRENLTIIRKSLNAAIEQELRRTADDRLFFQLTSLRTELNRDIQARLVQTEETAERTP
                     AEVLPALVLAASWYDDASRETDILDRNAISHPGFVPVRALRVPVR"
     gene            617..1705
                     /locus_tag="OQM83_003976"
     CDS             617..1705
                     /locus_tag="OQM83_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCS1522089.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLVLVLGENILSCDTERSIRERFSEYQVSGQRPG
                     NDDDFGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRE
                     TTYTVQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGP
                     ADAYLPEPVTARKKKMLRRISDERVFSS"
     gene            1683..2216
                     /locus_tag="OQM83_003977"
     CDS             1683..2216
                     /locus_tag="OQM83_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HCS1522090.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            2222..2632
                     /locus_tag="OQM83_003978"
     CDS             2222..2632
                     /locus_tag="OQM83_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCS1522091.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
BASE COUNT          608 a          661 c          795 g          614 t
ORIGIN      
        1 caatgtccgg cattacgctg gacccaggac tggcaccgcg tgcggtgtgg cccacagatt
       61 ccgcatcggt tatcaggcaa aaacagcaga caaaccttgt ggctgctgtc atccggacga
      121 cggcaatcag cgaagccaca agggcggtca cttcactgcc acaacccgga agcctggtga
      181 aaaatcagca ggcggttgtg gctgttggtg gttccactga acgtcagtcc gatattattc
      241 acgtttctca tccggcactt gacagcgtgg cagccagcac aggacaggat gagagagcgc
      301 aaccacccac gcgggaaaat ctcaccatca tacgcaaatc gctgaacgcg gcgattgaac
      361 aggagctcag gcgtacggcg gatgacaggc tgttttttca gctgacgtca ttacgtacag
      421 aactgaaccg ggatattcag gcgcgtctgg ttcagacgga ggaaaccgca gagcgaacgc
      481 cagcggaagt tctgcctgcg cttgttctgg ctgcgtcatg gtacgacgat gcgtcccgtg
      541 aaactgatat cctggatcga aatgccatct ctcatccggg ctttgttccg gtcagggcat
      601 taagggtacc cgtcagatga atgataccgt tttacttcgg gtttccggtc gcgaatgggg
      661 cggctggaca tccgtccgta tcagtgcggg cattaaccgt attgcccggg attttaatgt
      721 tgccattacc acccgctggc ccggaagccg ggattatcaa ccccggataa aaaatggtga
      781 gctggttgaa gtgcttatcg gggatgagcc tgtgctcacc ggatatgtgg aggcactacc
      841 gcttcgttat gacgccagca gcgtcagcat gggaattgtc gggcgaagca aaacagccga
      901 tctggttgac tgctctgctt tgccactcca gcagagcgga aaaaacctgc tcagaatagt
      961 cagtgaactg gctgcgccat ttggcatcac cgttgttgat gctggcgtgc cgcagacagc
     1021 ggtgattgat gcccagccgg aacacggcga aaccgttgcc gattgtctta accggttact
     1081 ggggcaggtt cagacgctgg cttatgacga tgaatgcggg cgactggttc tgggaaaacc
     1141 cggaacaggt aaagcggcga cggcgctggt gctggtgctg gtgctgggag agaatattct
     1201 ttcctgtgac acggaaagaa gcatcagaga gcggttttct gaatatcagg tcagtgggca
     1261 gcgcccgggc aacgacgatg attttggtga ggccaccatt gccgcaatac gtcagaccat
     1321 tcaggacagt ggcgtgaccc gttatcgccc tttgttgatt cagcagtcag gcacagcaac
     1381 gacagcaacc tgtaaggccc gttgtgaatt tgaagcgcgc caacgggctg cgcttacccg
     1441 tgagacaaca tatacggttc agggctggcg gcagggcagt ggcgcgttat ggcgtccggg
     1501 gttatctgtc atcgttttcg acccgctgaa taattttgat aatgatgaac tggtgatcgc
     1561 agaagttacc tataaccagg acgaccgggg cacgacgact gaattacggg ttggcccggc
     1621 agatgcttat ctccccgagc ctgttaccgc caggaagaaa aaaatgttga ggaggatttc
     1681 tgatgaacgg gttttctctt cgtaatctga tttcccgggc tgtcatcacg gcggtggatt
     1741 ccgccagaaa gtgtcagtct gtagggttga aaatgatagc cggagatcag aaacaacacg
     1801 ttgagcacct tgaaccttat ggttttacat ctgccgcaca gaacggtgct gagggcgttg
     1861 ctttatttcc ggcgggcgat cgttctcatg gtgtggttgt ggtcgtggct gacagacgtt
     1921 accggctgaa aggactgaaa cgtggggaag tggcgcttta tgacgatctg ggacagtccg
     1981 ttgtcctgac ccgttccggt attgtggtgg acggggccgg gaaggccata atttttaaaa
     2041 acgcgcctaa agcgcgcttt gaaatgccag tcgaatccac cgccgatatt actgacaatt
     2101 gcgacagtgg cggactcagc atgcagcaaa tgcggcaggc ctacaatgcc cacaaacata
     2161 ccgaaaatgg tgatggtggc gggatcactg acacgccgga tcaaccgatg ggctgaaaat
     2221 catgatgatt aatgttaacg ggcgacccgt gtcgaccggg gcttcgatcg accttctgac
     2281 gcgtgctgtg attatttcgc tttttacatg gcggcgtgcc gggcgtgatg atgcaccacg
     2341 gatatttgga tggtgggggg atacctggcc tgcggttcag aatgatcgca cggggtcgcg
     2401 tttgtatctg ttgcgacgca gcaagctgac aaataaaacc ccgcagcttg ccagagatta
     2461 tgtccgtgag gcgctggcgt ggatggtgga ggatggtgtt gcttcccgtc ttgatattaa
     2521 cgctgtccgg accgggacag actcgctggc acttgccatt accatttacc agcgtgacgg
     2581 caatattcac aacattattt ttgatgatat ctggagtgaa ctgaatggct gacagtcagt
     2641 tttatcgccc cggcctcccg caacttattt ctatgatc
//
LOCUS       DAJVQG010000264         2621 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJVQG010000264 DAJVQG010000000
VERSION     DAJVQG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.270"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(105..179)
                     /locus_tag="OQM83_003979"
     tRNA            complement(105..179)
                     /locus_tag="OQM83_003979"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Gln,seq:ttg)
     gene            complement(203..287)
                     /locus_tag="OQM83_003980"
     tRNA            complement(203..287)
                     /locus_tag="OQM83_003980"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(251..253),aa:Leu,seq:tag)
     gene            complement(296..372)
                     /locus_tag="OQM83_003981"
     tRNA            complement(296..372)
                     /locus_tag="OQM83_003981"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(336..338),aa:Met,seq:cat)
     gene            complement(752..2416)
                     /gene="asnB"
                     /locus_tag="OQM83_003982"
     CDS             complement(752..2416)
                     /gene="asnB"
                     /locus_tag="OQM83_003982"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCS1522092.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
BASE COUNT          635 a          664 c          651 g          671 t
ORIGIN      
        1 ggggtacctg gattcgaacc agggaatgcc ggtatcaaaa accggtgcct taccgcttgg
       61 cgatacccca ataaccggtc ggtgaaccgc ttactcgaag aagatggctg gggtacctgg
      121 attcgaacca gggaatgccg gtatcaaaaa ccggtgcctt accgcttggc gataccccat
      181 ccgtacaacg ctttctggtg aatggtgcgg gaggcgagac ttgaactcgc acaccttgcg
      241 gcgccagaac ctaaatctgg tgcgtctacc aatttcgcca ctcccgcaaa aaagatggtg
      301 gctacgacgg gattcgaacc tgtgacccca tcattatgag tgatgtgctc taaccaactg
      361 agctacgtag ccatcttttt ttcgcgatac cttatcggcg ttgcggggcg cattatgcgt
      421 atagagcctt gcagcgtcaa cctctttttc aaggaaaatt gctcgaaagt gactgtttgg
      481 ttaggttgcg aacagcgtgg cgctatattc gtcaattatt gtttactttg tgttttttcc
      541 ccaccctaca gccattcttt tgtcatacag gatgaaattc ggaattcaac aatggtggtg
      601 ggaaattaat ctatgaaata ctggcctaca gtgatgagtt gtcaaacagt gatgtggcaa
      661 acccggaaca tttccttact gcatatcaga atcaacaagc acctcaataa ctgaaacagc
      721 cccggatttc accggggctg tttcgcattt cttacttata cgccgactgg tgaacgccaa
      781 ccgcacgacc agacggatcg tccattttct tgaacgcctc atcccactca atcgctttag
      841 cggaagaaca cgcgacggac gggccgcccg gcacgcactc agcggcgctc ggaagcggga
      901 acagttcttc aaagatctcc cggtacagat acgcttcttt tgaggttggc gtgttgtacg
      961 ggaagcggaa gcgggcagtt tccagttgct gatcagaaac ctgctgcgcc gccacttctt
     1021 tcagggtgtc gatccaactg taaccgacgc catcggagaa ctgctctttc tgccgccagg
     1081 ccacgcttgc gggcaggtat gactcaaaac attcacgcag gatgtgtttt tccattttgc
     1141 cgttaccgca cattttatcc tgcgggttaa tgcgcatcgc cacatcaagg aattttttgt
     1201 cgaggaacgg aacgcgtgct tccacgcccc aggctgacat cgctttgttg gcgcgcgcgc
     1261 agtcatacat atgcagggcc agcagtttac gcaccgtctc ttcatgcagt tctttagcgt
     1321 tgggcgcttt atggaagtaa agataaccgc caaacacttc atcagaacct tcaccggaca
     1381 gcaccatttt aatgcccatc gccttgatct tacgcgacat taaatacatc ggtgttgaag
     1441 cgcgaatagt ggtcacatca taagtttcga tgtggtaaat cacgtcgcgg atggcatcca
     1501 gaccttcctg tacagtgaag tgaatttcgt gatgcaccgt gcccagatgg tttgccactt
     1561 cctgggctgc tttaagatcc ggtgaacccg gcagacctac agcaaaggag tgtaactggg
     1621 gccaccaggc ttcagagcgt tcctgatctt ccacgcgacg ggctgcgtat ttcttggtga
     1681 tagcggaaat aattgaggaa tccagaccac cagaaagcag cacaccgtaa ggcacatcag
     1741 acatcagatg gcttttaacg gaatcttcca gagcctgacg cagctcgttt ttgtcggtca
     1801 cgttatcttt cactgcatca aagtcgaacc agtcgcgatg ataataagaa cggatttcgc
     1861 cgtcctggct ccacaaatag ctccccgccg ggaactcttt aatcgtgcgg caaactggca
     1921 ccagcgattt catttctgag gccacataca gctgaccgtg ttcgtcatac cccatataca
     1981 gtgggatgat ccccagatgg tcgcgaccaa tcaggtaggc atctttttcg ctgtcgtaca
     2041 gggcaaaggc aaacatgccc tgcaagtcgt cgagaaattc cggccctttt tcctgataca
     2101 gcgcgaggat cacttcacag tcagaccctg tctggaactg ataacgatcg ccatattcag
     2161 cgcgcaatgc ctggtggttg tagatttcac cgtttaccgc cagtacgtgg gttttttgtt
     2221 ggttgtagag aggttgcgcc cccgcgttaa cgtcaacaat tgacagacgt tcgtgagcga
     2281 gaatggcgtt atcgctggca taaataccgg accagtccgg gccacgatga cgcatcaggc
     2341 gtgacagctc gagtgctttc ttacgctgct caactgcgtc tgttttgata tcgaatacgc
     2401 caaaaattga acacataacc ttctccgtta acctggtatt tgttgcttgt tgtgtttgct
     2461 tgtttaaaaa aatgccgcaa agcagcactg tgcgcaagcg atttggcggt gaaaaaataa
     2521 aaaacgtaat ggtgattgtc gattggtgaa aaagggtctg gtgtgaggat gtatttattg
     2581 atgaatcgat aatttttagc gggttttatt gaatggttat a
//
LOCUS       DAJVQG010000265         2619 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJVQG010000265 DAJVQG010000000
VERSION     DAJVQG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.271"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            125..1305
                     /gene="nepI"
                     /locus_tag="OQM83_003983"
                     /pseudo
     CDS             125..1305
                     /gene="nepI"
                     /locus_tag="OQM83_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(1346..1639)
                     /locus_tag="OQM83_003984"
     CDS             complement(1346..1639)
                     /locus_tag="OQM83_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522093.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            1861..>2619
                     /gene="nlpA"
                     /locus_tag="OQM83_003985"
     CDS             1861..>2619
                     /gene="nlpA"
                     /locus_tag="OQM83_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCS1522094.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVALGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQS"
BASE COUNT          608 a          583 c          673 g          755 t
ORIGIN      
        1 gttccgtttc gatcttaact ccagctcaag caacaccgta aaagttgcaa aaacgttaaa
       61 tgcgtcacac atttcaatgt taaagtggtc ggcttttccc ctgaaacatg ccacgggtaa
      121 caccatgagt gaatttattg ccgaaaaccg cggcgcggat gccatcaccc gaccgaactg
      181 gtcagccgtt ttctcggtgg cgttttgtgt cgcctgtctg attatcgttg agtttttgcc
      241 cgtcagtttg ttgacgccaa tggcccagga tttaggcatt tcggaagggg ttgccgggca
      301 atcggtgacc gtgaccgcct ttgtggcaat gtttgccagt ttgtttatta cccagacaat
      361 tcaggctact gaccgccgca acgttgttat tttgtttgcc gttttgctga cgctctcctg
      421 cttgctggtt tcctttgcta actcattcag tttgctttta atcggtcgtg cctgtctggg
      481 gctggcgctg ggcgggttct gggcgatgtc ggcgtcgctg accatgcgtc tggtgccgcc
      541 gcgtacggtg ccgaaggcgc tgtcggtgat cttcggcgcg gtttctattg cgctggtgat
      601 tgccgcgccg ttgggcagtt ttttaggcga gcttatcggt tggcgcaatg tctttaatgc
      661 ggcggcggtg atgggcgtgc tgtgtatttt ctggattatc aaatcattgc cttcactgcc
      721 aggcgaaccc tcgcatcaga aacaaaatac tttccgctta ttacaacgtc cgggtgtgat
      781 ggcagggatg atcgccatct tcatgtcttt cgccgggcag tttgctttct tcacatatat
      841 tcgcccggtg tatatgaacc tggcgggatt cagcgtggat ggcttaacgc tggtgctgtt
      901 gagttttggc attgccagct ttatcggtac gtcgctttcg tcattcattc ttaaacgttc
      961 ggtaaaactg gccttagcag gcgcgccgtt aatactggct gtgagtgcgt tggtgctgac
     1021 gttgtgggga agcgataaaa tcgttgctac cggcgtggcg attatctggg ggctaacttt
     1081 tgcattggtt cccgtcggct ggtcaacgtg gatcacccgc tcactggccg atcaggcaga
     1141 aaaagccggg tctattcagg tggcggttat tcagcttgtg gcgcggcaat cggcggttat
     1201 gcgctggata atattggtct gacttcgccg ttgatgtttt ctggcacatt gatgttgctg
     1261 actgcattgt tggttactgc aaaggtgaaa atgaagaaat cctgatatca ggtaaagaag
     1321 cgttggtcag tagttgttgc tgttcttaca tatccgggca ttctacctta cccaacgctt
     1381 cccaataatt gctctgatta ttacgttcca tcgccacgat ggcctcgcgt atatgttgtt
     1441 tattgttttc atctgacaac attaactttt cccacgcttc atctgataat gacgcctgtg
     1501 gcgtgcaaat tttacgtaaa tcttgtaata ccgtggtttt ctcttttgcg gactgcagtg
     1561 agctaaaagg cgactcggcg tatacgatgc ctggcatagc gaaaaaagtg aaaataagca
     1621 gtagtgtcgt tggtttcatg gattcaacct tttacaaatg tgtatcgaat gaagcataag
     1681 tgtagtttgc tttcatcgct ctcatcatgc tgtacgaaac gcgcttgcgt ggtcagtaag
     1741 aagtgccaga ctttatattc cacttttatt cctttttatt cttatcgata gcgtttcgtt
     1801 ttttaaaccg cagcgacctt accgctatag tcaggtaatc attaataaaa ggataaaaaa
     1861 atgaaactga caacacatca tctacggaca ggggccgcat tattgctggc cggaattctg
     1921 ctggcaggtt gcgaccagag tagcagcgat gcaaaacaca ttaaagttgg cgttataaat
     1981 ggcgcagaac aagatgtcgc ggaagtcgct aaaaaggtgg caaaagagaa atatggcctc
     2041 gatgttgaac tggtaggatt tagcggttcg ttgctaccga acgatgcgac aaatcatggc
     2101 gaacttgatg cgaatgtctt ccagcatcga ccctttcttg aacaagataa tcaggcgcat
     2161 ggttataaac tggtagcgct gggaaatacg tttgtcttcc ctatggcggg ttattccaaa
     2221 aaaatcaaaa ccgtggcgca aataaaagaa ggcgcgacag tggcgatccc caacgacccg
     2281 accaaccttg gtcgcgcact tttactgttg caaaaagaga agctaattac tctgaaagag
     2341 ggaaaagggt tattacctac cgcgctggat ataaccgata atcctcgtca tttgcagatt
     2401 atggaactcg agggggcgca gctcccgcgc gtactggatg atcccaaagt tgatgtagcg
     2461 attatcagca ccacttacat tcagcagaca gggctttctc cggtgcacga cagcgtattt
     2521 attgaagata agaattcgcc gtatgtgaat attttggtgg cacgggaaga taataagaat
     2581 gcagaaaacg tgaaggaatt tctgcaatct tatcaatca
//
LOCUS       DAJVQG010000266         2593 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJVQG010000266 DAJVQG010000000
VERSION     DAJVQG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.272"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(1120..1455)
                     /locus_tag="OQM83_003986"
     CDS             complement(1120..1455)
                     /locus_tag="OQM83_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HCS1522095.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSVFFADTPGKMGLLAVYTGTVFILSLPLSMIYILS
                     VIIKRLSVR"
     gene            1584..1931
                     /locus_tag="OQM83_003987"
     CDS             1584..1931
                     /locus_tag="OQM83_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522096.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(1951..2541)
                     /locus_tag="OQM83_003988"
     CDS             complement(1951..2541)
                     /locus_tag="OQM83_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522097.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     WVVRREGDPLEVAQWLPACHDAGMEVRVQINESAAP"
BASE COUNT          739 a          610 c          533 g          711 t
ORIGIN      
        1 agtcacataa accaccgatt gagtgacagt tactgaaaca actatttcga cacaaaatct
       61 cgtctattca acatcatgca aatttgtcag tgactgaatt tagtgacaga accctctgga
      121 attctgccgt gtaaccactt atatgcagac ctgccccgtt gctctgggtt ttcaggctgc
      181 gatgagtgat gagcgatgat ttccatacag atgatatatc gactggaatt gattgcctgc
      241 aattagattt agttttattg tgtggatcgt tttagttagt ggggatgaat ttccgataaa
      301 ttaaccaatg gaaaggagat gatatgaata aaaagataac tggattatta ataattagtc
      361 tggcgtcaat tttcttaatt gattgcaccc agtgccatta acatctatct acacaaaaat
      421 agaggaaaac aattttgata tatcaaataa ttacaagcag ctccattctt tttattgttt
      481 gggggattac atctataata attagaacaa agaattattt ttctaagaag aaaacagacc
      541 ttaatataag cttttttata gccttcccta tttctataac aatcctttta ttttgccgag
      601 gtatattata aatataacat aatacatcta tatggggtgt acatatcccc acataaaaac
      661 acatactctc attaacacgg gaagaaacta tgttttccaa ttctcactac atgtacataa
      721 tcgttcttgt actatggttt agtattctca agtacttaca gagaaagaat agcatattac
      781 atcctttatt ttcactaatc ctgacaccac taaccatttt aatcattaga cttatgatag
      841 atttaacata caaccaataa attataaaca aaataaaaat agggaacatt atatgcttta
      901 taaaattttc acattaaaca caaagataaa aacaactatc attacaatat gtgcattatc
      961 atataatctt acagcgcaag cttccccgtc atacagacct cgtgatcgag ttaataagac
     1021 tatgatagaa ctacaaacta acggagtaga acggcaactt ttagataatt cttgtggcat
     1081 aagttctggg gtatttaaat aaaatataag acaggctgtc tatcttacag acagcctttt
     1141 tataataaca gaaagaatat atatcatact taacggaaga gataatatga aaacagtacc
     1201 cgtataaaca gcaagcaatc ccatttttcc aggtgtatca gcaaaaaaaa cgctgttcca
     1261 gaaatcaggc cgggtaaatt tcagagctgt atcttcaata taccattttg caaccggata
     1321 caaaaccatt ccgcagagag aaatacacca gaacagtaat ctgtatttaa aatcataatc
     1381 ccatgacata tacagcatat atcctcccgt cacccatccc caccacatat tattaaaata
     1441 atattttctg ttcatctgcc agttttcctt tcttttgcac ttttgtcagt gtactgatgc
     1501 atgacaacat aaccacagca tgataataat aatcaataac aataagctgt gtcacgttta
     1561 catcatggaa cggaggtgct cctgtgactt ttgagcagaa aaaagcccgg gccattgccc
     1621 tgatggacag caaaaagatg tggcggagca attatgcccc tcctcttctg cgtattctct
     1681 ggcggctggg tatccggctt ccgccattac cctttatgcc gttctggcag gtcacggtgc
     1741 tgacgggggg cttatggggc atttcctggg gatgtgcaat gtggtttatc tactgggggc
     1801 cgtcaggaat ggtggcaggt gaggccatta ttataagtat cacggggggt tttttgtccg
     1861 gactgctcat ggcctctttc cactggtggc gcaggaaagt taaccgactg ccaccatggg
     1921 atgatgtgta aaccgtgcac aggcgtgttc ttacggagcg gcactttcat ttatctgtac
     1981 gcgaacctcc attcctgcat cgtgacaggc cggcagccac tgcgccactt ccagcggatc
     2041 gccctcccgg cgtaccaccc agccttcttt attccataac tgcagacagg tgctgccgtc
     2101 gagacgcacc acaaaatccc cacggcaggc ctgatacgtt atgccgttca gctccaccgc
     2161 cagatcagtg gcagaagtgc cgcctgcggg cagacctgct gccgggacca gcggcagtac
     2221 gacgacttct gccgcacgct gttctgcccg gatgcggtcc tgttctgcca gcagcagagg
     2281 ctgtgccacg gcccggccgg cgtccgtcag ctccactgcc agctgcaggt tcggagcgcg
     2341 cagggtacgc agccagccgg catcctccag acgacggcag gaggcgcgca ggtttggccc
     2401 gtagaccggg gcttccccgc tttgctccag cacccgttct atgtcccgcg tcagcacggg
     2461 tcctggccgt ttttcatcaa gggcagccag cacaatcagc actctccgct gcagtggcga
     2521 cggtcggcgg ctctgagtca tttccagtca tcctccggcc agatgagctt tctgaggcgc
     2581 tgatggtcgg cgt
//
LOCUS       DAJVQG010000267         2573 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJVQG010000267 DAJVQG010000000
VERSION     DAJVQG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.273"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..461)
                     /locus_tag="OQM83_003989"
     CDS             complement(<1..461)
                     /locus_tag="OQM83_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1522098.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNY"
     gene            complement(461..1381)
                     /locus_tag="OQM83_003990"
     CDS             complement(461..1381)
                     /locus_tag="OQM83_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1522099.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1519..2418
                     /gene="pgrR"
                     /locus_tag="OQM83_003991"
     CDS             1519..2418
                     /gene="pgrR"
                     /locus_tag="OQM83_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCS1522100.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
BASE COUNT          655 a          598 c          582 g          738 t
ORIGIN      
        1 tcgtaattgc tgaaaccttc ccgaaaaaga cgcccgatat taacacccgt aatactcact
       61 actgctttaa ttcgtttttc tgtcagcgcg gcattaatgg catagccacc acccccacac
      121 actcccagca cgccaatacg ttcagcatta acatatggca gagttactgc ataatcgata
      181 acacggctaa tatcctctac gcgctgtgtc ggatcttcta tccagcgagg ctccccacca
      241 gattctccct ggaaactggc gtcataagcg agaacgagat agcctttttc agccaatgcc
      301 ttgccataga tattgccaga cgtctgctcc ttacaactgc caaaaggatg gacgctgata
      361 atcgccggat actgttttga ctcatcaaaa tttggtggaa actgaatatt ggcagcaata
      421 tgccaggcca tatcactatt gcggatttga atatgtttca ttacagagtt tcctcaaaga
      481 acggagctaa tactgaaatt gcttcagcca cataggcttt gccgtcataa agatccatat
      541 ggtttgcgcc ttcaacaatg tgatagcgtt tatcttggct tgatgctcga tcgtacaggt
      601 cgtcactcat ccatttgctc cctgcctggc tgcccgccac aatctgcatc ggctgagtca
      661 ggtacacttc cgccatatgg taagcatcaa aggtaataat ctggttaagg ctgcgcaaag
      721 tagcgtaacc cggtgctgtt ggatactgcg cgcgaggggt gtggtaatac tcccaggcct
      781 gacgcagttc ttcgttcggc gcatcggact ccttcattgg tgccagtggc ataacggcat
      841 attctccgct gctaatatcg ctggttcggg cgtttgaacc cgcttcaacg tatggcaggg
      901 catcaataga tttcacattg ttttcccaac cattacggaa catcgaacca atattgaccg
      961 cactaacggt accgatggcc ttgatgcggc gatcctgaat tgcagcattg gctgtatatc
     1021 ctgcaccggc acaaattccc atcgcaccaa ttcgggtatt gtcgacataa gaaagcgttg
     1081 tcaggtaatc aatcacggca ctgatgtctt cagtacgaat gtatgggttt tctaactgac
     1141 gcggctcgcc gccactttca ccttgataag atgcgtcata agcaatagtg acaaatccct
     1201 tttccgccag ttttttggca taggttccgg ccgtttgttc tttaacgccc ccacctgggt
     1261 gagataccac aattgcctga tactgacggg tttcatcaaa ttttggaggg aaatagatca
     1321 ctgcagacaa agagatggtt ggattattgc tgttagtgaa gctgacttta ttattcatca
     1381 ttccgttcct catggagttg tcggttcgtt ttaacggttg gtgatatcac tatagatatt
     1441 gatcattaag ttgattagac ccaaaattat gattagacct atcatttaaa tgattaatag
     1501 attgcatgga gatactgaat gaagcgtgaa gaaatcgctg atctgatggc gtttgtcgtc
     1561 gttgcagagg agcgtagctt cactcgtgca gcagcccgcc tgagcatggc gcagtcagct
     1621 ttaagccaga tagtgcgtcg tatagaagaa cgattgggat tgcggcttct gacgcgaacc
     1681 acgcgcagcg ttgttccaac tgaagcgggc gagcatcttt tgtctgttct tggccctatg
     1741 ttgcatgaca tagattcagc cctggcatcc ctgagcgagc tgcagaaccg cccatccggg
     1801 acaatacgta ttactactgt agaacatgca gccaaaacga tattgttacc agcaatgcgc
     1861 acattcctga aatcgcatcc tgaaattgat attcagctca ccattgatta tggtttgacc
     1921 gatgtcgttt ctgaacgttt tgatgcaggc gtccgtctgg gtggggagat ggataaagat
     1981 atgatcgcca ttcgaatcgg gccagatata ccaatggcta ttgttggctc accggattat
     2041 ttttctcgcc gaagtgttcc aacgtcagtg tcacaattaa tagatcatca ggcaattaat
     2101 ttgtatcttc ccacatcggg tacagcaaat cgctggagat taatacgcgg tggacgtgaa
     2161 gttcgtgttc gcatggaagg tcagctttta ctgaatacga tagacctgat cattgatgct
     2221 gcaattgatg ggcatggatt ggcgtatcta ccttatgatc aggttgagcg ggctattaaa
     2281 gaaaaaaaac tgatacgtgt tttggataaa ttcacaccag atttacccgg ttatcacctg
     2341 tactatccac accgtcgaca tgctggctcg gcattctcat tatttataga taggctgaag
     2401 tataaaggtg ctgtttagca ctacttgctg atacattaat ttaatttttc tcttaacgta
     2461 ttctcagttc ctttcaacgt tttggttatt ttttattctt cgtacaatgg cgacagatgc
     2521 tgattatgat accgaaaact ggtttgaacg tgcgaagcct gaacgtagtg ttc
//
LOCUS       DAJVQG010000268         2460 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJVQG010000268 DAJVQG010000000
VERSION     DAJVQG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.274"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(26..316)
                     /locus_tag="OQM83_003992"
     CDS             complement(26..316)
                     /locus_tag="OQM83_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCS1522101.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(306..743)
                     /gene="ratA"
                     /locus_tag="OQM83_003993"
     CDS             complement(306..743)
                     /gene="ratA"
                     /locus_tag="OQM83_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCS1522102.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            914..1396
                     /gene="smpB"
                     /locus_tag="OQM83_003994"
     CDS             914..1396
                     /gene="smpB"
                     /locus_tag="OQM83_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCS1522103.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1611..1973
                     /gene="ssrA"
                     /locus_tag="OQM83_003995"
     tmRNA           1611..1973
                     /gene="ssrA"
                     /locus_tag="OQM83_003995"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2172..>2460)
                     /locus_tag="OQM83_003996"
     CDS             complement(2172..>2460)
                     /locus_tag="OQM83_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCS1522104.1"
                     /translation="FEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWGDADNQQV
                     KLSMPELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLGTIRAFDVT"
BASE COUNT          672 a          659 c          549 g          580 t
ORIGIN      
        1 tcaatgagca ccttttttct gtctgttatt tattcgctga tttttctgct cgttgcctgc
       61 gaagctcttt cggatcggca atgagagggc gataaatctc cacccgatcg ccatcatgca
      121 cactatcgct tagttttgcc ggacggctgt aaatgccgac tttgttttta gttaaatcga
      181 tatcggtacg caattccagc aagccactgg cgcgaatagc ttcttcaacc gtcgcgccct
      241 cctgcagcgt cacgcgctgc aggtactgct tctcaggtag cgcataagcc acctcaacgg
      301 caattttacc tagcactgta aacctctttc gcacgaaccg taaaagcctg gaccatatta
      361 gacgccagct ctttaaacac gcgaccaaag gcgagttcaa tcaacttatt ggtaaactca
      421 aagtcgagat gaaactcgat acgacacgcc tcctggctca gcggcgtaaa cttccatcca
      481 ccaatcaatt tcttgaacgg cccatccacc agattcataa gaatactttg gttactggtc
      541 aactggttgc gggtagtaaa cgttttgctg atcccagcct tagagacatc taccgccgca
      601 gtcatctgcc caggagtgga ctccagaatc cgacttccgg tacaacccgg caaaaactga
      661 ggataagact gaacgtcatt cactaactga tacatttgct ccgcgctgta gggtaccagt
      721 gcggtccggc taatctgagg cataactatt tccatcaaca aaaatccaac aaataatatc
      781 atttatcctg ctgtaaaaaa aacgctatcc cggcgctggg taacatcggg ttcatgctaa
      841 gatagagcct tgtcccccgc aggattgata tggggtgttt tcgatttcag attaccgatg
      901 attcacgacg cttatgacga agaaaaaagc acataaacct ggttcagcga ccatcgcgct
      961 taacaagcgc gcccgtcacg aatactttat cgaagaagag ttcgaagcgg gacttgccct
     1021 gcaaggctgg gaagttaaat ccctgcgcgc aggaaaagcc aatatcagcg acagctacgt
     1081 ccttctgcgt gacggagagg catttctgtt tggcgctaac atcacgccaa tggccgtggc
     1141 ctccacgcat gtggtgtgcg atcctacccg tacccgcaag ttacttctca accagcgcga
     1201 actggactca ttgtacggtc gcgtcaatcg agaaggctat accgtagtgg cgctctccct
     1261 gtactggaaa aatgcctggt gcaaagtgaa aatcggcgtc gccaaaggta aaaaacagca
     1321 cgataaacgt tcagatatca aagaacgcga atggcaggtg gataaagcac gtatcatgaa
     1381 aaacgcccac cgttaaacct gcactccaat tattgaccag ttcctcaccg cgcctccctc
     1441 tccggcggcg cgaatgaaca tcttattggc tatcacatcc gacacaaatg ttgccatccc
     1501 attgcttaat cgaataaaaa tcaggctaca tgggtgctaa atctttaacg ataacgccat
     1561 tgaggctggt catggcgctc ataaatctgg tatacttacc tttacacatt ggggctgatt
     1621 ctggattcga cgggatttgc gaaacccaag gtgcatgccg aggggcggtt ggcctcgtaa
     1681 aaagccgcaa aaaatagtcg caaacgacga aaactacgct ttagcagctt aataacctgc
     1741 ttagagccct ctctccctag cctccgctct taggacgggg atcaagagag gtcaaaccca
     1801 aaagagatcg cgtggaagcc ctgcctgggg ttgaagcgtt aaaacttaat caggctagtt
     1861 tgttagtggc gtgtccgtcc gcagctggca agcgaatgta aagactgact aagcatgtag
     1921 taccgaggat gtaggaattt cggacgcggg ttcaactccc gccagctcca ccacttttga
     1981 caggactgca accggacaga gactataaaa acagtcactt acggacactg accagacagt
     2041 aagcggacca gaaaaagaca aaaatataca cgcaaaatgc acgtgcattt caaaaaagcc
     2101 tctgattatg aagtcagagg cttttctagt tgtaaataag ggtaacaatt taacacgatg
     2161 cagcttacca attacgtaac atcaaacgct ctaattgttc ccaaatcctc cagaccactc
     2221 agctcctctt tcatttcacg ctgacggcga taaatctcat cgttgcgatc gacctgcgcc
     2281 tgcaccattg ctgccgccag ttcttccagt tccggcatcg acagtttcac ctgctgatta
     2341 tcggcatcac cccacgccat atgcgtttgt gctgtgacag attttgccag catgactacc
     2401 ggggacaggc ggcccagtga gtcggggcca gcattccaga tacgaccatt ccattcaaac
//
LOCUS       DAJVQG010000269         2436 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJVQG010000269 DAJVQG010000000
VERSION     DAJVQG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.275"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(189..1532)
                     /gene="gdhA"
                     /locus_tag="OQM83_003997"
     CDS             complement(189..1532)
                     /gene="gdhA"
                     /locus_tag="OQM83_003997"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCS1522105.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            1768..2040
                     /locus_tag="OQM83_003998"
     CDS             1768..2040
                     /locus_tag="OQM83_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCS1522106.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(2006..2413)
                     /gene="nudG"
                     /locus_tag="OQM83_003999"
     CDS             complement(2006..2413)
                     /gene="nudG"
                     /locus_tag="OQM83_003999"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCS1522107.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
BASE COUNT          604 a          653 c          593 g          586 t
ORIGIN      
        1 cactacagta agaaatgccg ttggcccata agaaatgttc cctggggagc atcatccgtt
       61 aaatactcat aaacgcctga aattttgccg ggggcgctgc gcttgcccgg cctacattag
      121 agcgtaactg caatgaattg tgcccatttg taggcctgat aagcgtagcg ccatcaggca
      181 tttacaactt aaatcacacc ctgcgccagc atcgcatcgg caaccttcac aaaaccggca
      241 atgttcgcgc cctgcacgta gttggtttgc tcaccttcac caccatgctc aacacaggca
      301 tggtggatat ccagcatgat gtgatgcaaa cgtgcgtcaa ctttctcggc tttccagccc
      361 aggcgcgcag cgttttgtgc catttccagg cccgacgttg ctacaccgcc agcattagcc
      421 gctttacccg gtgcaaacag tacgcctgcc tgctggaaca gttcagtcgc ttcgatggtg
      481 gtcggcatat ttgccccttc agcgacggct ttaacgccat tggcgataag ctgatgcgcg
      541 gcgtcaacat ccagttcgtt ctgggtggcg caaggcaggg cgatatcaac cggtacagac
      601 cacggctgtt ggccttcgag atagaccaga ccaaattctt tggcgtaatc tgccactcga
      661 ccatcgcggc tggctttgat ttcgataaga cgtgccagtt tctctttcgt gaatccgctt
      721 tcatcaacta cagtgccgct ggagtctgac gcagtgatca cacgagcacc aaattccatc
      781 gctttttcga tagcgtactg ggcgacgttg ccggagccag aaacggaaac gcgcatccct
      841 tcaaaaccca taccgtggcg ttttaacatt gcttctgtga aataaaccag accgtagccg
      901 gtagcttccg ggcgaataag actaccgcca aatgaaaggc ccttaccggt gaagacgcag
      961 gcggtattgt tggagagctt tttcatcatc cccgccataa agccgacttc acgaccacca
     1021 acgccgatat cacctgccgg aacgtcggta tccgcgccca gatgacgata aagctcggtc
     1081 atcagcgcct ggcaaaaacg catcacttca ccttcgcttt ttcctttcgg atcgaaatcg
     1141 ctgccgcctt taccaccgcc catcggcagg gtagtcaggg catttttgaa ggtttgttca
     1201 aagccgagga atttgagaat ggaaaggtta actgacggat ggaagcgcat accgcctttg
     1261 tacgggccga tggcagagct gaactgcaca cgccatgcac ggttgacctg tatctggttg
     1321 cgatcatcaa cccataccac gcgaaactgg atcacgcgct ccggttcaac cagacgctcc
     1381 agtaatgaca tctggcgata ttttggattt tgttcaagaa aaggccagag tgtggtcatt
     1441 acttcacgaa cggcttgcgc gaactcggtt tgattcgggt cgcgcttttg gacatggttg
     1501 aggaatgact ccagagaata tgtctgatcc atagatataa aacccttata tattaatacg
     1561 attgtgctta tgttgtttat gtcatgtgct tttgcagttt tcgactatac catcaagaat
     1621 gtgacggtaa gcaagaaaaa aatgacccgg gaaagcaaat taataacgag agtaatctca
     1681 taaccaaatg cgataactca gacgctaaag ttttgccacc cggctaccgt tatcagttgc
     1741 agagacaata acgttaagga gttccctgtg agtcgagcat tgttcgccgt agtattagca
     1801 tttccattaa ttgcactggc taacccgcat tatcgtccgg acgtggaggt aaacgtaccg
     1861 ccagaagtgt tcagttcagg cgggcagtca gcgcagccct gtactcagtg ctgcgtttat
     1921 caggatcaaa attattctga aggggcggta attaaagccg aaggaatact gctgcaatgc
     1981 cagcgggatg acaaaaccct tagcactaat ccgctgctct ggcggcgcgt aaagccataa
     2041 acgcctctaa tagtggaatg tcagcagggg ccagcggata ttgcagcgcc tcttcaggtg
     2101 agcaccagac cagcgcctga tgttcatgtg cctgtaacgt cccgtggaag tcgggtacgt
     2161 gccaggcatg aagatggata atccggcccg aaacttctcg ctgatggctg gcaacatact
     2221 cacccacagt tgcttcgatg cccaattctt cgtttaactc acgcaccagt gcctgctgct
     2281 ggctttcatc cagctcgact ttaccatcgg caaactccca taatcccgcc tgatcgctct
     2341 gggcggggcg ttgcgcgagt aaaattttgc catcacgttc aatgatggcg gcaacaactt
     2401 caatcatttt cataaattac acacgggcag ataagc
//
LOCUS       DAJVQG010000270         2421 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJVQG010000270 DAJVQG010000000
VERSION     DAJVQG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.276"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..755)
                     /gene="ydcO"
                     /locus_tag="OQM83_004000"
     CDS             complement(<1..755)
                     /gene="ydcO"
                     /locus_tag="OQM83_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HCS1522108.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAG"
     gene            808..1344
                     /gene="sutR"
                     /locus_tag="OQM83_004001"
     CDS             808..1344
                     /gene="sutR"
                     /locus_tag="OQM83_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HCS1522109.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1417..>2421
                     /gene="rlhA"
                     /locus_tag="OQM83_004002"
     CDS             1417..>2421
                     /gene="rlhA"
                     /locus_tag="OQM83_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HCS1522110.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     A"
BASE COUNT          604 a          607 c          626 g          584 t
ORIGIN      
        1 tatccagctg ctttcattgc tgcgataccc ggtgcgtttt gcgatgccat cgtcaccaga
       61 aaaaggggga gtgcaacgct caggctgtga gcaaacgaaa aatcaggggt aatataagtg
      121 gggagaacgg gtttaaagac aacatcagtt gtgacaacgt caccttgcgc gataacgatc
      181 acgatcccaa taatcatcgc ggcaattacc gcatagcgcg gcgcaacggc cttggttgcc
      241 agccatacca gcaacatact tccacacaac gtaaattgac cgtccagact ggcaaacgcc
      301 tgtaaaccaa agcgtaataa aatcccggca agcattgccg ccgcaagcga gtgcggaata
      361 atgcgcatca gacgagcaaa gagtcccgtt atgccgcaga ggactattaa cgcgttggtg
      421 acaataaaaa cgccgatggc ttcgttaagt gttagtccct gcaatccggt gaccaacaaa
      481 gccgcgccag gcgttgacca tgcggtgaga acaggtacgc gataccataa tgtcagagtc
      541 agcgtactga cgcccattgc cagccccagc gccgtcatcc agccagagat ttgtgcagtg
      601 gtggctccgg cgacaatcgc tgcttgccag attattgccg ctgaactggc gtagccaatt
      661 aataccgcca gaaaccccgc cagtagcgtg ggtggaggaa tagaaaacag acgcatagtc
      721 acctcgtgcg ttatagcgta caggcaccgt agcatttgtc cgttataacg cacaagtgat
      781 aaacttccgt tttgccggag gagtcgcatg gaaaatctcg ctcgcttttt atccactaca
      841 cttaaacaac tacgccagca gcgcggctgg agtctttcgc gactggcaga agcgacgggc
      901 gtttctaaag caatgttagg gcagattgag cgtaatgagt ccagtccgac ggtcgcgaca
      961 ttatggaaaa ttgccaccgg gttgaacgta ccattttcaa catttatttc tccgccgcag
     1021 tccgctatgc cttcagttta tgatccacaa cagcaggcga tggtgatcac ttcgctgttt
     1081 ccttatgatc cacaactctg ctttgaacac ttctcgattc agatggcatc tggtgcaata
     1141 agcgaatcga cgccccatga gaagggggta atcgaacatg tcgtcgtcat tgatggacaa
     1201 cttgatctgt gcgttgatgg ggaatggcag acccttaatt gtggggaagg cgttcgattt
     1261 gctgcagacg tcacgcatat ctatcgtaat ggtggggagc aaaccgtaca ttttcattcc
     1321 ctcatccatt acccgcgcag ttaagcggga aaactatttc gcaacgtcgc gcttgtggct
     1381 aaaatagccg ccatttttca gctactggat aagaatgtga ccgtatcttc tcatcgactt
     1441 gaactgttaa gcccggcacg cgatgccgcc attgcccgcg aagctatttt gcacggtgcc
     1501 gatgctgttt atatcggcgg ccctggtttt ggtgcccgtc ataatgccag taatagcctg
     1561 aaagatattg ccgagctggt gccgtttgcc catcgttatg gtgcaaaaat tttcgtcacg
     1621 cttaacacca ttttgcatga tgatgagctg gaacccgcgc aacggctgat tactgacctc
     1681 taccagaccg gtgtcgatgc gctgattgtt caggatatgg ggattctgga acttgatatt
     1741 ccgccgattg aactgcacgc cagtacgcag tgcgacattc gtacagttga aaaagcgaag
     1801 ttcctctctg atgttggctt cacgcagatt gtgctggcgc gagagctgaa tcttgatcag
     1861 atccgcgcga ttcaccaggc tacggacgcg accattgaat tctttattca tggggcactg
     1921 tgcgtggcct attcgggtca gtgctacatt tctcatgcgc aaacagggcg tagcgccaac
     1981 cgtggcgatt gctcgcaggc gtgccgtttg ccatacacat tgaaagacga tcaggggcgg
     2041 gtggtttcct atgaaaaaca tctgctgtcg atgaaagata acgatcagac tgccaacctc
     2101 ggcgcgctga ttgatgctgg cgtacgctcc ttcaagattg aagggcgtta caaagatatg
     2161 agctacgtga agaatatcac cgcccattat cgccagatgc tggatgccat tattgaagaa
     2221 cgtggcgatc tgacgcgtgc ttcatcaggt cgtactgaac atttctttgt tccatcgacg
     2281 gaaaagactt tccaccgtgg tagcacagat tattttgtga atgcccgtaa aggcgatatt
     2341 ggcgcgttcg attcgccgaa atttatcggc ctgccggtgg gtgaagtatt gaaagtggcg
     2401 aaagatcatc tcaatgttgc c
//
LOCUS       DAJVQG010000271         2402 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJVQG010000271 DAJVQG010000000
VERSION     DAJVQG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.277"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(63..1481)
                     /gene="phrB"
                     /locus_tag="OQM83_004003"
     CDS             complement(63..1481)
                     /gene="phrB"
                     /locus_tag="OQM83_004003"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCS1522111.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGVGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPIIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDLEGEFIRQWLPELRNVPGKSVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(1478..1987)
                     /locus_tag="OQM83_004004"
     CDS             complement(1478..1987)
                     /locus_tag="OQM83_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCS1522112.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT          610 a          621 c          553 g          618 t
ORIGIN      
        1 gtaggctgga taaggcgttc acgccgcatc cggcatctgg gctcgattgc ctgacgcgtc
       61 tgttatttcc ccttccgcgc cgcctcatac gctgccaacg tttgtactct cgcctctttg
      121 tgctcgacta tcggttgcgg ataatccagc ttcacacctg ctttctgcgc ccacttccac
      181 ggctcatgca ccgatttccc cggtacattg cgtagttccg gtagccactg gcggataaac
      241 tcaccttcga gatcaaattt ctcgccctgg gttgtcgggt tgaaaatacg aaaatacggc
      301 gctgcatcgg ttccggttga agcggcccac tgccagccac cgttattggc tgccaaatca
      361 ccatcaatca gctgcgacat gaaatatcgc tcgccttcgc gccagtcgat caataaatct
      421 ttcaccagaa aactggctgt aatcatccgt agcctgttat gcatccagcc agtgctgtta
      481 agctgacgca tagcggcatc aacaatcggg tatcccgttt tgccttcctg ccaggcctgt
      541 aaatgtgcgg gattgctctg ccactgtaca cgatccgtcc aggcaataat tggacgatgt
      601 ttacacaacg aggggtgata cgttatcagg tgacggtaaa actcgcgcca gatcagctca
      661 ttaagccaga cactaccgac cccaccgtcc agcgcctgcg gctgttcagc caacaagcga
      721 tgcaagcact ggcgaggcga taacccgccc gttgccaggc tggccgacaa acggctggtg
      781 ccttccactg ccggaaaatc tcgttgttgc tcatattctc cggcaccgtt ctggcaaaac
      841 tggcgtaatt gcgcaatcgc cgctttttct tccaccggaa aatgcgcagt atcgaaagac
      901 tgacgaggat aattcagcgt aatggatggc gagggctcta tcgatccgct actacgaact
      961 tttggcgcag cgacgcactc cggcatccct tcccgcagcc gtttcagcca ggcattctta
     1021 aaaggcgtaa agactttgta catctcgtga ttaccggtca tcaccgcgcc aggcggcagg
     1081 atcacgctgt catcaaatcc ttcacacacc acgttacgca gcgctctttc aacttccaca
     1141 tcccgcgccc gctcattcac ttcatactga tagttataaa acaggtgggt aacgctgttt
     1201 tccgcgcaca cctgtttaac tatttcgaca ctggcgacaa agtcatccac ttcacggaac
     1261 aataaaggaa tacctttttc cgcaagcgct atttgtagcc cattcagttg agcattgata
     1321 agttcagcct gacgcggcga catgttatgc gtcgcccact ggcgtggtgt agcgatatac
     1381 aacgccagca cgcgtgcaga cgaattgcgg caggcggcag ccagtgcgag attatcgtgc
     1441 agacgtaaat cttggcgaaa ccagaccaga tgggtagtca taaaactcct gaatggcgca
     1501 agttaatcac acgcggccaa agttcgaaat aacgctgccc tgaaagccag gcgtcatgat
     1561 aaagcgccat atagtgtttg atacgcatca gcggcgcaag aagtggttgc tcgccacggc
     1621 gataactgtc gataagcgca gccagctgct ggcgttctgt ggaatcaata tgcgggcgaa
     1681 aataaccctg aacgtgcatc aaaacattgg tgtgatcgcg cgggttggcg gggtgagaaa
     1741 gcaaaacaac tacgcgttgg cggtactggt tgtaatagtc atccagattt tgccactggt
     1801 gaatatcagc cacgaacggg ccaagtttgc ggtactccgg ctgagaatgc gccagaaaga
     1861 cgagtttata gcggctgtga taatcgagaa gcgccccgcg cgttaagcca tgttctttca
     1921 gtcgatgtaa ctcatgaagg gcaaagatac ggattagggt gctgtcatca aatcgttgta
     1981 gattcattac tgctcctgaa cggtataaag gagtaagtgt agacgatggt aaatgaattt
     2041 tatggggcag agtttttgcg gggaagtatt ttgttggtaa aaatattatc aacttcatct
     2101 gagtgagaat tattctacta tttcccgagc tgcattcatt ttttgtattt tttattttta
     2161 gaaaataaca gttctcaaag cttaattacc tcactattaa ttgtatttca aactaacaat
     2221 cgcactccat tttctctttt taaaaaacat aaacactgtt aaaacaaata cttatttcgt
     2281 tatcttaaat gctatttcta catcactcgt ccttataatc aaattattct tattcatagc
     2341 tatttttgca ggtttaaaat agcggctttc accaaagtca gccatgaaaa ttagactaca
     2401 ag
//
LOCUS       DAJVQG010000272         2399 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJVQG010000272 DAJVQG010000000
VERSION     DAJVQG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.278"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..856)
                     /locus_tag="OQM83_004005"
     CDS             complement(<1..856)
                     /locus_tag="OQM83_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HCS1522113.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
     gene            complement(913..2091)
                     /gene="allB"
                     /locus_tag="OQM83_004006"
                     /pseudo
     CDS             complement(913..2091)
                     /gene="allB"
                     /locus_tag="OQM83_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            complement(2010..>2399)
                     /locus_tag="OQM83_004007"
     CDS             complement(2010..>2399)
                     /locus_tag="OQM83_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCS1522114.1"
                     /translation="LTGTRQALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWAD
                     KIGHLQIADNPHRGEPGTGEINYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDP
                     YFLNRVVATGKVMKPVLAQRQKVVSPP"
BASE COUNT          622 a          664 c          587 g          526 t
ORIGIN      
        1 atccggtcgc cacaaagctg cgacgataac gcgtattccc cgcgccctgc tgcggataaa
       61 aaacatccgt gccccgaatc gcaccgtagg tattgctgat atttaccaga cctgtaatca
      121 ccgccgtcag aataattccc ggcgacaaag caccgccact gccgagcggg aaccactgcc
      181 agtgcaactc accggagagc gaatgcgaag aaggaaagca aaagtaccac aacaaccagc
      241 cggttatggt gccgaccagc aggccataac gggcaaaacg ttgcggcaga aagataatca
      301 tcgccagtac caggcacatc accgccaccg agagcgcgaa cggtggtaac tgaattttaa
      361 aatatgggtc ggctatgcca aacggcagcc cgagcatacc tttgaaaaag atagtggtca
      421 gctgtgcgcc cagcatcaac ataaacaaga ccatcaccga cggcgtaaat aaccgtgcca
      481 ggcgatggcc taatccgcta aaaccaatca acatcgtcag cacgccggag agtgcaatcc
      541 ccaccgccag actggtagcg atatcgttga tcggtgtccc gcgcgatgct tctccaaggg
      601 tgatagtaag gatggttccc caccacaggc cacccggccc ttccataata gcgcgacgat
      661 gaccgcagaa cgcctgagcg aagcaggcca gtgcggtagc aagaaaagca tattgcgtga
      721 gcgtaagcag gctactttgc ggcaactgaa aagcagaaag tagcgtagga ggaaccacaa
      781 ccgtgttgca aaaaataaag aaaaaccact gaaatcctga taacaggctt tcgcggctga
      841 ctgcaaaatt gaacataaac acaccttaac cggagaatgc ccgccgcaag gacgggcatt
      901 gcaggggcca gattactgct gatgtttaag gataaattga cctttcggcg caacagggaa
      961 gccctgttca atgtcgtaaa tcacatcacc acgtaagatg gttttcgtga tacgcgcgcc
     1021 aatggtacgg ccaacatacg ggctgacttt gtggcgatat tccagatcgt cattggtaag
     1081 aacatagctg ctattcggct gaatgaagac gaagtcggca tcttttcctg gggcgatacg
     1141 gcctttttgc tgcagaccga aaatatctgc tgcgttagtc gccattaatt tgccgaacat
     1201 tggcagagac attccgcgtt tctgtaccgc ttcatcgaac atcacgtcca tacagttttg
     1261 cagaccggca ataccgcccc atgcttccat gatgttgccg gctttcattt ccggagggca
     1321 tggtgagtgg tcggaaacca ggcagtcgat ttcaccgtta aacagttttt cccacatgcc
     1381 tttctggttt tccagatcgc ggatcggcgg tgaacacttc gccagagtac cgatttcttc
     1441 gaactgatcg gtatccagta caaagtaatg cgggcaggat tcacaggtaa catcctgacc
     1501 ttcctgacgt gcacgagtca cttcttcaac actttccggg ctgctgatat ggcaaacgtg
     1561 cagacggcaa ccggcaactt tcgccagata cagcacgcgg cgaatcgctt ccacttcggt
     1621 aaataccgga cgcgaagcca catagtcatg ggcagttacg cgaccttcac gtttcgcttc
     1681 ttcgcccggt gcgtcacaga tcagcgcgtt ttcgcagtgc accaacaccg gctgccccag
     1741 ttcgcccagc ttctgcgcac ctttgaagaa ctgccagtcg tttacatcac ggaagtcgtt
     1801 gtcgataccg cgatcgccac aggtcgcaac gaagcatttg aagccgacaa cgcccacttc
     1861 atccagctca tgcagacggt cgatgttgta agacaccagg ccaccgagtt gtgccgcatc
     1921 gatagtcagc ttgcctttag cggcatcgaa cttcagttca attgaagcgc ggtcaaccgt
     1981 tgcaggcagc tggttgagcg gcatttcgat catggtggtg ataccacctt ttgccgctgc
     2041 gcgagtaccg gtttcataac cttcccagtg gctacgaccc ggttcagaaa gtacggatcc
     2101 atccagcgta aaccggcttc cgtggtggtt tggggtttat attcacaccc aacccaaccg
     2161 ttgtagtcag aattttcgat taccttaaag agataatcat aattaatttc tccggtcccc
     2221 ggttcgccgc gatgcggatt atcagcaatt tgcaggtgac caattttatc ggcccactga
     2281 gtcatggtgt tggttaattc accttccatc cgctgcatgt gataaatgtc atactgaatt
     2341 ttcaaattgc agcaaccaac atcatcaatc aatttcagcg cctgccgagt tccggtgag
//
LOCUS       DAJVQG010000273         2381 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJVQG010000273 DAJVQG010000000
VERSION     DAJVQG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.279"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..571
                     /locus_tag="OQM83_004008"
     CDS             <1..571
                     /locus_tag="OQM83_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HCS1522115.1"
                     /translation="YTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRADELVIEID
                     PGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFFLDQLEAL
                     GYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTPIRTWGDT
                     VAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            558..1598
                     /locus_tag="OQM83_004009"
     CDS             558..1598
                     /locus_tag="OQM83_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1522116.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRLATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDTMTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEG
                     QGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGDN
                     DTGFKQNGDGLLDVYANSVHVLRFQSGSIQSNKAVNVTGRVTPSDYGNFDARYQQRNG
                     GVQDVRYGYEMYYTPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRPLQK
                     LINGTWYNVASI"
     gene            1601..2146
                     /locus_tag="OQM83_004010"
                     /pseudo
     CDS             1601..2146
                     /locus_tag="OQM83_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            2170..>2381
                     /locus_tag="OQM83_004011"
     CDS             2170..>2381
                     /locus_tag="OQM83_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCS1522117.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEY"
BASE COUNT          623 a          514 c          672 g          572 t
ORIGIN      
        1 ttatacaaag ctgctgtacg gactgatgcc gccggggcct gcatggtcag ataccgacgg
       61 tgtacttgac ggtctggcac catcgcttgt gcgtgttcac cagcgggctg atgaactggt
      121 gattgaaatc gatcccggtc agtccacaga gctgattgaa cgttatgaag aattgtatgg
      181 tttacctgat tcctgttccc ctgtcggaac ccagacatta cgccagcgtc agcaacgtct
      241 tgaagcaaaa gccaatgtgg ctggtggcat aaatgagcag ttcttcctgg atcagcttga
      301 ggcgctggga tataccggcg taacgatcga acagttccag cacttggatg caagccccga
      361 tccggaatgg ggagatcgct ggcgttattt ctggcgtgtg acgttgccga tggatgccgg
      421 tgctcagtgg cagacatgca cggacgcctg caacacaccg atccggacgt ggggggatac
      481 ggttgcggag tgcgtgatta ataaattatg tccgtcacac accgtcgttt tattttccta
      541 tccagatgag gatgaagatg cacaggattg atacgctgac tgcggtaaaa gataagtttg
      601 gtccgggtaa gaacggattt actgacggaa atcttcgcac aggacgtctt gctacctggc
      661 tgaacagtgc catgtggaat gccattcagg aggaaatctg tggtgtcatt gagaaggccg
      721 ggatagaact gaataaagaa gaacacgacc agctatataa ggctatatta ttgctggtgg
      781 gcggtgcaat taacgaagag gcattgctga taaaaaataa cctttcggat gtggaagaca
      841 gggatgaggc tgttgaaaac ctcggattaa aacccacggt ggataaggca aaaaatgccg
      901 ttcagcgtga tggcgacacc atgaccggag agctgaaaat ccgtggtgtt aatgcgctga
      961 ggattttcaa cgaagctttt ggcctgattt ttcgtcgttc ggaagagtgc ctgcacctta
     1021 ttcccaccag tgaaggtcag ggggaaaatg gcgatattgg tcccctgcgc ccgttcacca
     1081 ttaatctgcg gacgggtgaa atatccatgt cgcataaagt gtctgttggc ggtggttcgc
     1141 aggtcaatgg tgcgctgggt atcggcgttc agaacgccct gggggggaat tcaattgttc
     1201 ttggtgataa tgacaccgga ttcaaacaga atggtgatgg cctgctggat gtttatgcca
     1261 atagcgtgca tgtgttgcgt tttcagagtg gcagtatcca gagtaataaa gctgtaaacg
     1321 ttacaggacg ggtaacaccg tcagactacg gaaactttga tgcccgttac cagcagcgaa
     1381 atggtggcgt gcaggatgtg cggtatggtt atgaaatgta ttacaccccc ggcagtaaca
     1441 ccgtttcgtg gacatttcgc tcaccttcgg gacacgggct gtcagggata tcgatatcgg
     1501 ataccggccg taactcagcg gataacgtca acggcgtgta ttaccgaccg ctgcaaaaac
     1561 tgattaatgg cacctggtat aacgtagcga gtatttaaca atgttgcatt taaaaaatat
     1621 tacggcaggt aatccgaaaa ccgcggaata atatcagctg acaaaacagt atgatgtcac
     1681 ctggcttttt tcggaagacg gcaaaaactg gtatgaagaa cagaagaact ttgccagtga
     1741 caccataaaa atggtttaca ccggagacgg gcgcgtggtg tgggtcggta aggatgtgac
     1801 aggcattgaa ccccgtaacg ccagtgttat tgaagttcct gatattaccg ccaaccgccg
     1861 cattaccgcg ccgggttact ggttttaccg caatgatgaa tttgtctttg actacagact
     1921 caaagcggaa gatgagcgtg atgcccttct ggctcaggtc agtgcccgga caggggaatg
     1981 ggaagaagac ctgctgctgg ggctaatcag cgacgaagac cgggaaaaac tgaaagcgta
     2041 ccgtatttac gcgaaatcgc tgcaggcgat ggatttcagc accatcactg ataaatcctc
     2101 atataacgcc attgaatggt ccgtctctcc ggaaggttct tcctgattta atttatcgcg
     2161 agaaaaacta tgtctgtagt gatatcaggt gcgctgactg atggcgcagg catccccatg
     2221 tccggatgcc agattattct gaaatcccgt gtaaacacct cagaagtggt gatgcgtacc
     2281 agggctgatg tagtgaccgg aaataacggt gaatattcgt ttgaggcaca ggtcggaaaa
     2341 tattgcgtgt atctgaaacg ggactggcgc gacgagtact g
//
LOCUS       DAJVQG010000274         2297 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJVQG010000274 DAJVQG010000000
VERSION     DAJVQG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.280"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..923
                     /gene="tyrR"
                     /locus_tag="OQM83_004012"
     CDS             <1..923
                     /gene="tyrR"
                     /locus_tag="OQM83_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCS1522118.1"
                     /translation="AVSPKMKHVVEQAQKLAMLSAPLLITGDTGTGKDLFAYACHQAS
                     PRAGKPYLALNCASIPEDAVESELFGHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQA
                     KLLRFLNDGTFRRVGEDHEVHVDVRVICVTQKNLVELVQKGMFREDLYYRLNVLTLNL
                     PPLRDCPQDIMPLTELFVARFADEQGVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYR
                     ALTQLDGYELRPQDILLPDYDAATVAVGEDAMEGSLDEITSRFERSVLTQLYRNYPST
                     RKLAKRLGVSHTAIANKLREYGLSQKKNEE"
     gene            complement(967..1473)
                     /gene="tpx"
                     /locus_tag="OQM83_004013"
     CDS             complement(967..1473)
                     /gene="tpx"
                     /locus_tag="OQM83_004013"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCS1522119.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            1592..>2297
                     /gene="ycjG"
                     /locus_tag="OQM83_004014"
     CDS             1592..>2297
                     /gene="ycjG"
                     /locus_tag="OQM83_004014"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCS1522120.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGVAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLK"
BASE COUNT          571 a          540 c          656 g          530 t
ORIGIN      
        1 tcgccgtcag cccgaaaatg aagcatgttg tcgaacaggc gcagaaactg gcgatgctaa
       61 gcgcgccgct gctgattacg ggtgacacag gtacaggtaa agatctcttt gcctacgcct
      121 gccatcaggc aagccccaga gcgggcaaac cttacctggc gctgaactgt gcgtctatac
      181 cggaagatgc ggtcgagagt gaactgtttg gtcatgctcc ggaagggaag aaaggattct
      241 ttgagcaggc gaacggtggt tcggtgctgt tggatgaaat aggggaaatg tcaccacgga
      301 tgcaggcgaa attactgcgt ttccttaatg atggcacttt ccgtcgggtt ggcgaagacc
      361 atgaggtgca tgtcgatgtg cgggtgattt gcgttacgca gaagaatctg gtcgaactgg
      421 tgcaaaaagg catgttccgt gaagatctct attatcgtct gaacgtgttg acgctcaatc
      481 tgccgccgct acgtgactgt ccgcaggaca tcatgccgtt aactgagctg ttcgtcgccc
      541 gctttgccga cgagcagggc gtgcagcgtc cgaaactggc cgctgacctg aatactgtac
      601 ttacgcgtta tgcgtggccg ggaaatgtgc ggcagttaaa gaacgctatc tatcgcgcac
      661 tgacacaact ggacggttat gagctgcgtc cacaggatat tttgttgccg gattatgacg
      721 ccgcaacggt agccgtgggc gaagatgcga tggaaggttc gctggacgaa atcaccagcc
      781 gttttgaacg ctcggtatta acccagcttt atcgcaatta tcccagcacg cgcaaactgg
      841 caaaacgtct cggcgtttca cataccgcga ttgccaataa gttgcgggaa tatggtctga
      901 gtcagaagaa gaacgaagag taagcgcgaa tatgcctgat ggtgcaacac catcaggcat
      961 attaacttat gctttcagta cagccagagc tgcttcgtaa tccggctcgg tggtgatttc
     1021 atccaccagc tggctgaaaa tcacattgtc attttcgtca ataaccacaa cggcacgcgc
     1081 tgccagacct ttcagtgggc catcagcaat tgccacaccg taagcttgca gaaattcagc
     1141 gttacggaaa gtggagaggg tgataacgtt gttcagacct tctgcgccgc agaaacgaga
     1201 ctgggcgaac ggcagatcgg cagagataca cagcacaacg gtgttgtcga tctcagttgc
     1261 cagttggtta aacttgcgta ctgatgcggc gcaaacatcg gtatcaatac tcgggaaaat
     1321 gttcagcact ttgcgtttac ccgcaaactg accgagggtg acgtcagaca gatcttttgc
     1381 cacgagagta aaagtctgcg ctttgctacc cgcctgcggg atggaattgg cgactgtaac
     1441 cgggttgccc tggaaatgaa cggtttgtga catgattatc tttcctgttt acatatagtt
     1501 aacgtcacac ctagtttatg ctaactgtca ataacacagc aaacgctatt tgcgcttaat
     1561 ccgcagacca ccgcgacaac aaggagtaaa gatgagaacc gttaaggtat ttgaggaagc
     1621 ctggccctta cataccccgt ttgtgattgc ccggggaagt cgcagtgaag cgcgcgtggt
     1681 ggtggttgaa ctggaagaag agggtattaa aggcaccggc gaatgcacgc cgtatccgcg
     1741 ttatggagaa agtgatgcct cggtaatggc gcaaattatg agcgtcgtgt cgcaattaga
     1801 gaaagggctg acacgggagg agttgcaaaa aattctccct gccggcgcag cacgtaatgc
     1861 gctggattgt gcattgtggg atctggccgc gcgaaaacag cagcaatcgc tggctgattt
     1921 gatcggcata acgcttcccg agacagttat cactgcacag acggtagtca tcggtacgcc
     1981 tgatcagatg gccaatagtg catcaacact ctggcaggca ggcgcgaaat tactgaaagt
     2041 gaagctggat aaccatctta ttagtgagcg gatggtggca attcgcacag ctgtgcccga
     2101 tgcgacgctg atcgttgatg caaatgaatc ctggcgtgca gaaggggtgg cggcgcgttg
     2161 ccagctattg gcggatttag gcgttgcgat gcttgaacaa ccccttcctg cgcaggatga
     2221 tgcggcgctg gagaatttta ttcatccgtt gccgatttgt gctgatgaga gttgtcatac
     2281 acgtagcaat ctgaagg
//
LOCUS       DAJVQG010000275         2267 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJVQG010000275 DAJVQG010000000
VERSION     DAJVQG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.281"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..188
                     /locus_tag="OQM83_004015"
     CDS             <1..188
                     /locus_tag="OQM83_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1364 family protein"
                     /protein_id="HCS1522121.1"
                     /translation="AGLCGTGIKPPDLIATIACSACHDEIDRRTYFVDAEYAKECALE
                     GMARTQVIWLKEGVIKA"
     gene            185..547
                     /gene="rusA"
                     /locus_tag="OQM83_004016"
     CDS             185..547
                     /gene="rusA"
                     /locus_tag="OQM83_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HCS1522122.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            544..684
                     /locus_tag="OQM83_004017"
     CDS             544..684
                     /locus_tag="OQM83_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HCS1522123.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            770..1153
                     /gene="quuD"
                     /locus_tag="OQM83_004018"
     CDS             770..1153
                     /gene="quuD"
                     /locus_tag="OQM83_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HCS1522124.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(1342..>2267)
                     /locus_tag="OQM83_004019"
     CDS             complement(1342..>2267)
                     /locus_tag="OQM83_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCS1522125.1"
                     /translation="GDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDK
                     TRLAFAGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVA
                     TYRNNDFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSD
                     RTNNQVIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKA
                     QNFEVVAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVD
                     YKINLIDKSDFTKASGVATDDIVAVGMVYQF"
BASE COUNT          626 a          481 c          549 g          611 t
ORIGIN      
        1 tggctggatt gtgcggtacc ggtatcaaac cgccagacct gattgccacc attgcatgtt
       61 ctgcctgtca cgacgaaatc gaccgccgca catattttgt cgatgctgag tatgcaaaag
      121 aatgcgcgct ggaaggtatg gcgagaacgc aggttatctg gctgaaagag ggggttatta
      181 aggcgtgaat acctacagca tcacattacc ctggcctccg agcaataacc gctattaccg
      241 ccataatcgc gggcgcacac acatcagcgc agaagggcag gcataccgcg ataacgtcac
      301 ccgaatcatt aaaaacgcaa tgctggatat cggcctggct atgccagtga aaatccgtat
      361 tgagtgccac atgccggatc gccgtcgccg tgacctggat aatctgcaaa aagccgcttt
      421 tgacgcactc accaaagcag gtttctggct ggatgatgct caggtcgttg attaccgcgt
      481 tgtgaagatg cctgttacca aaggtgggag gctggaactg accatcaccg aaatggggaa
      541 tgaatgatgt ttgagtttta tatggcagaa cttcttcgcc accgctgggg gcatctgcgc
      601 ttatatcgtt tccccggttc tgttttgacc gattaccgaa tactgaagaa ttacgccaaa
      661 accctgacag gagcaggagt atgaagtcag agataacaat caactaatac tgttttgttg
      721 atttttgctt gtaattggcg ttctggtctg atttttgtgg agtaagttga tgcgtgatat
      781 tcagatggtt cttgagcgtt ggggagcgtg ggcggctaat aatcatgaag atgtgacctg
      841 gtcgtccatt gccgccggtt ttaagggatt aattacttca aaagtaaaat ctcgcccgca
      901 atgttgtgac gatgacgcga tgattatttg cgggtgcatg gcccgtctga aaaagaacaa
      961 cagcgatttg cacgatttat tagtagatta ttatgtagtc ggtatgacat tcatgtcact
     1021 ggcaggtaag cattgctgct ctgatggtta tatcgggaaa aggttacaga aggctgaggg
     1081 cataattgaa gggatgttaa tggcattaga tatccggtta gagatggata tcgttgttaa
     1141 taactctaat taatatgcca attgtttact aaaaattatt aaaaatgggg cgttgcaacg
     1201 cccccaaaaa taaagggtaa tatataacag aaggtttata tagttagaag caaggttgtg
     1261 ctcctaaagg aagtggcttg agggagccac ttatatgttg gggaggcaaa gcctcccgca
     1321 acatatcttt tagtaatcaa attagaactg gtaaaccata cctacagcaa cgatatcatc
     1381 ggtagcaacg ccagatgctt tcgtgaaatc gctcttatca atcaggttga ttttgtaatc
     1441 aacaaaagtg gacatatttt tgttgaagta ataggttgca cctacatcaa tatattcaac
     1501 caggtcctga tcaccccacg cacccaagtc ttttccttta gattgcaggt aagcaacgga
     1561 cggacgcaga ccgaagtcga actgatattg tgcaactact tcgaagtttt gtgctttgtt
     1621 ggcaatatgg ttattaccaa aaacagtcat gttctgggtt tcagaatagg tggtagccag
     1681 atagatgttg ttcgcatcat atttcagacc agctgcccat acttcagcat tttgaccaga
     1741 tgcattcagg ctgttgttac cgtagataac ctgattatta gtgcggtcag atttagcata
     1801 ggttgcacct acaccgaatc cttcatactc ataagtagtg gagaaaccga aaccatcacc
     1861 attagcttca gttacgtcag tgcggtcatt tttaccctga tactgagcag caaagttcag
     1921 accatcaacc agaccaaaga agtcgttgtt acgataagtt gcaacaccag tggtgcgacc
     1981 agtcatgaac acatctgttt gagtccaagt gtcaccaccg aattctggca ggacgtcagt
     2041 ccacgcaccg atgtcgtatg ctacaccgta gttacggccg taatcgatgg agccgtagtc
     2101 accgaatttc aggccagcga aggcaagacg ggttttatct ttggaggaac cttgagattc
     2161 agcgcggttg cctttgaatt catattccca ctgaccgaaa ccagtcagtt gatcgttgat
     2221 ttgggtttca cctttgaagc caagacgggc ataagtagta tcaccat
//
LOCUS       DAJVQG010000276         2144 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJVQG010000276 DAJVQG010000000
VERSION     DAJVQG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.282"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..1007)
                     /gene="shiA"
                     /locus_tag="OQM83_004020"
     CDS             complement(<1..1007)
                     /gene="shiA"
                     /locus_tag="OQM83_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HCS1522126.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
     gene            1322..>2144
                     /locus_tag="OQM83_004021"
     CDS             1322..>2144
                     /locus_tag="OQM83_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HCS1522127.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIK"
BASE COUNT          663 a          475 c          487 g          519 t
ORIGIN      
        1 gccataaaga aaggaaatgc gctcaacgtt ccgattaacg cacctgtgat ataaacccta
       61 cggcgaccaa aacgatcggc aagccaggca aaacagggaa ttgtcaggca gcttaatcca
      121 cctaccagca aaccaatatt aaggaaaagt tcgcgcggta gccccatatt ctgggttgaa
      181 taattaagtg caaaggcagt aacgatgtac atcgtcagca attcgcacag tcgtagcgca
      241 ataatcttca ggaaagcacc gggatgtcgt aacagcgctt cgataaccgg gatgcgtttt
      301 ttcgcggcag cttgataatg ttgctgttgt tcaaattccg cggactcctc catgccattg
      361 cgcacccaca atgctcccag taccagtacg atgctaaaca ggaaaggaat gcgccagccc
      421 cagcttaaaa actgttcgtc agtcgtcatc atactgatca atgaaaccag tccggttgaa
      481 agcagtaaac ctacaccgta gccaacttgt acaccgctac tgtaaaaggc ttttttattt
      541 ttcggtgcac tttcaacgga aagcaacgcc gcgcctcccc attcgccgcc gacggcaaat
      601 ccctgaatga cacgcagtgt caccagtaaa ataggtgccc accacccaat ggtcgagaat
      661 gaaggaagaa taccaatcaa ggctgtcgcg atgcccatca tccagacggt cagcattaac
      721 atgcgcttac gtcccagtcg gtcgccaaag tgaccgaaaa tgacaccgcc gagcggacgg
      781 aaaaggaatc cgacgccaaa ggtagcaaat gcggcgagcg ttcccatcgc cgggcttact
      841 tgcgggaaaa actcgcgatt aaacaccagt gcggcggtga tgccatagag taaaaaatca
      901 taccagtcga cgacggcacc agcgaagctg cctaacgcag ctcgtcgggc gcgacttaac
      961 gaaagcgtcc cttcatcggg acgagtggag atgagcgtgg agtccataat gatcctgttt
     1021 gtactgtctt tttataatta gtatggaaaa ggtaactcac attgccgtcg atctataaac
     1081 gaaccttatg agactacctc gacagagcgc atctgcaaat gattttatgg ctgattagcg
     1141 gcaacatgaa aaattcgatg gaagatgaaa gcgcaggagt tgctggtgtt gtttaaggat
     1201 taagcagagg acccacaggt tagagatatg aaacataaaa gtccgcatat gcacaaaatg
     1261 gcaccctgat gagtctgata gactgcattg cattataacc agtagggagg cgaggttagg
     1321 tatgttttta gcatcattgt tgagacgtat tgcatttagt tactacgatt ataaagctta
     1381 taatttcaat attgaaaaaa cagactttgt tgtcatccat attcccgatc agattggcga
     1441 tgctatggcc atctttcctg ttattcgggc gcttgaattg cataaaatta agcatctttt
     1501 aattgtaacg tcgacaatta atttagaagt ctttaatgcg cttaaacttg aacagactaa
     1561 attaacatta gtcacaatga ctatgcagga tcacgcaaca ttaaaagaaa taaaagattt
     1621 agcaaagaac ataacacagc aatacggtac gccggatctt tgtattgagg ggatgcgtaa
     1681 aaagaacctg aaaacgatgt tatttatcag tcagttgaaa gcaaaaacga attttcaggt
     1741 tgttggtata accatgaatt gctactcccc tttgtgcaag aacgcgtccc gtatggatca
     1801 gaaactccgg gctcccgtac ctatgacatg ggcatttatg atgcgtgagg cgggttttcc
     1861 agcagtcagg ccaatatatg aattgccact aagtgaggat gtactcgatg aggtgcgcga
     1921 ggaaatgcga tcgttaggat cttacattgc gttcaattta gaaggtagct cgcaggaacg
     1981 tacattttca ttatcgattg cagaaaatct aatagcaaaa attcaaagtg aaacagatat
     2041 accaatagtg atcgttcatg gacccaaagg tgaagataaa gccagggcat tagtggattg
     2101 ttataataat gtctaccgtt tatccttacc accctcgatt aaac
//
LOCUS       DAJVQG010000277         2143 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJVQG010000277 DAJVQG010000000
VERSION     DAJVQG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.283"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            41..127
                     /locus_tag="OQM83_004022"
     tRNA            41..127
                     /locus_tag="OQM83_004022"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:75..77,aa:Leu,seq:cag)
     gene            complement(166..402)
                     /locus_tag="OQM83_004023"
     CDS             complement(166..402)
                     /locus_tag="OQM83_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCS1522128.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            543..1331
                     /gene="fhuF"
                     /locus_tag="OQM83_004024"
     CDS             543..1331
                     /gene="fhuF"
                     /locus_tag="OQM83_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCS1522129.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1369..2046)
                     /gene="bglJ"
                     /locus_tag="OQM83_004025"
     CDS             complement(1369..2046)
                     /gene="bglJ"
                     /locus_tag="OQM83_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCS1522130.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(2004..>2143)
                     /locus_tag="OQM83_004026"
     CDS             complement(2004..>2143)
                     /locus_tag="OQM83_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCS1522131.1"
                     /translation="AIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSH
                     S"
BASE COUNT          562 a          561 c          477 g          543 t
ORIGIN      
        1 gcaccaaacg aggcgatatc aaaaaaagta agatgactgt gcgaaggtgg cggaattggt
       61 agacgcgcta gcttcaggtg ttagtgtcct tacggacgtg ggggttcaag tcccccccct
      121 cgcaccaatt atctttactt cctttcttgt ttcttccttg atttttcatc cctgattcaa
      181 attcattatc atcagcatga tggcagcaat aagcgccagg cacgatggta gcaaaacgta
      241 atgccgtaac tgtttgtgat acagcatcac cggcgtcaat actaatccca tcagaatgac
      301 aatccgccac tgatccggcg ataaatccgc atacatcagc cctgcaatca gcaatcccgg
      361 cagcaacact ccccagccac tgcccagcgt acgttgcaac atggtttcat ctctttcatt
      421 gataatgata accaatatca tatgataatt tttatcattt gcaagccaga gaaatccctt
      481 gctatcgggt aaacctatcg ctaggattag caatcattat catttagatt actatcccga
      541 ttatggccta tcgttccgca ccgctctatg aagatatcat ctggcgaacg catctccagc
      601 cgcaggatgc tgggcttgca caagccgtac gtgcgacgat cgccaaacat cgtgaacatt
      661 tgctcgagtt tatccgcctg gatgaacccg ctccgcttaa cgccatgacg ctggcgcaat
      721 ggtcatcacc gaatgcgcta agttctctac tcgcggtcta ttccgatcat atctatcgca
      781 accaaccaac gatgatccgc gagaacaaac cgctgatctc actatgggca caattgtata
      841 tcggcctgat ggtgccacca ttaatgctgg cgctactgac gcaggaaaag gcattagatg
      901 tgtcgccgga acatttccat gctgagtttc acgaaaccgg acgcgtcgcc tgtttctggg
      961 tcgatgtgtg tgaagataaa aacgcaacac cacattcgcc gcagcagcga atggaaacgt
     1021 taatcagcca ggcgctggtt ccggttgtgc aggcactaga agcgaccgga gagatcaacg
     1081 gcaaacttat ctggagtaat accggttatt tgatcaactg gtatctcact gagatgaagc
     1141 aactgctcgg cgaggcgacg gttgaatcgc tgcgccatgc cctctttttt gagaaaacgc
     1201 tcacgaacgg tgaagataat ccactctggc gtaccgtcgt gctgcgcgat ggcctgctgg
     1261 tgcgccgaac ctgctgccag cgttatcgct taccggatgt gcagcaatgt ggcgattgta
     1321 cgctgaaatg aaagcactgc cggggaagta aacccggcat catgcggatt aatagggatg
     1381 caacacatta cttgcttcgc aatgccgcat acataacaga atatctgcgg cctccaacaa
     1441 ccctgcatca gaactgacgc ccagtttcga catcacatta aatttatgtg cacggatcgt
     1501 tttgatattg cgtttaagct gctcggcaat ttgtgtcatt gagtagccac gcgacataaa
     1561 gcgcaatatt tctctctccg tcgggcttag cgtccggctt tggttaatgt accactgatt
     1621 gttcagtcgg tcggtcgcct gacgtacacc atttaatgac aaaaagagtt cctgatgaaa
     1681 aatctccagc gttgacgctt tacttaatac accgtccagc ggtgatggcg acaatgaacc
     1741 aatcagccga gcttcaatat catcatccgc aataactaaa cgccgggtac gcggaaactt
     1801 aatcgccagt tcagtcaggc aagataatcc ctctctgcgc tcacttctca tggcagaaaa
     1861 agaaaaaatg accgccgcaa atgagatccg cgacatggca tcctgaaatg actcctgact
     1921 ggtataggta tggagcttat aagggttacc cgcaaacttt ctgaataaac tctcaatacc
     1981 gatactgctc atgacgcatt tttctatgag tgcgacattt ctcttcttaa ttcggctgtg
     2041 ttccattctg cggcatctcc ataaacagaa ggcaagttaa gttctcttgc accctgcgcg
     2101 ctatttatcc atgcatacat ttcagcgttg ctgtgaattg cca
//
LOCUS       DAJVQG010000278         2089 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJVQG010000278 DAJVQG010000000
VERSION     DAJVQG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.284"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(60..1025)
                     /locus_tag="OQM83_004027"
     CDS             complement(60..1025)
                     /locus_tag="OQM83_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCS1522132.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQIAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="OQM83_004028"
     CDS             complement(1879..>2089)
                     /locus_tag="OQM83_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522133.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          577 a          549 c          437 g          526 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcaatc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag acttgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcactccc gaacggctga gcccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAJVQG010000279         2088 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJVQG010000279 DAJVQG010000000
VERSION     DAJVQG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.285"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            81..1253
                     /gene="istA"
                     /locus_tag="OQM83_004029"
     CDS             81..1253
                     /gene="istA"
                     /locus_tag="OQM83_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HCS1522134.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1253..2050
                     /gene="istB"
                     /locus_tag="OQM83_004030"
     CDS             1253..2050
                     /gene="istB"
                     /locus_tag="OQM83_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HCS1522135.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVESGQHQ"
BASE COUNT          552 a          505 c          590 g          441 t
ORIGIN      
        1 acggtaattc gccatttgga ttgtccgctc cacccaacat gttgtttcct taaggttctc
       61 acaccagaaa ggacatcaac atgctgagca gagaggactt ttacatgata aagcaaatgc
      121 gccagcaggg cgcgtacatc gtcgatattg cgactcagat tggttgctct gaacggacgg
      181 tcagacgcta cctcaaatac cctgaaccgc cagccagaaa gacccgccac aaaatggtta
      241 agctgaaacc gtttatggat tacatcgaca tgcgcctggc agagaatgtc tggaatagcg
      301 aggttatctt cgcggagatt aaggcaatgg gttatacggg cggacgttcc atgctgcgtt
      361 actacatcca gcccaaacgt aaaatgcgtc cgtcaaaaag aacagttcgc ttcgaaactc
      421 agcctggata ccagctccag catgactggg gcgaagttga ggtggaggtt gccgggcaac
      481 ggtgcaaagt taactttgcg gttaatacgc tggggttctc ccgcagcttc catgtcttcg
      541 ccgcaccaaa acaggatgct gagcatacct acgaatcact ggttcgcgcc ttccgctact
      601 tcggtggttg tgtgaaaacg gtgctggttg ataaccagaa ggctgcggtg ctgaagaata
      661 acaacgggaa agtcgtgttc aactccggat tcctgttgct ggctgaccac tataacttcc
      721 tgccacgggc atgccgtcca cgcagggcca gaacaaaagg taaggttgag cggatggtga
      781 aatacctcaa ggagaacttc ttcgtccggt accgcaggtt cgacagcttc actcatgtca
      841 atcaacaact ggagcaatgg atagccgatg tggctgacaa acgggagctt cgccagttca
      901 aagaaacgcc ggaacagcgc ttcgcgctgg agcaggaaca tctgcagccg ttaccggata
      961 cagacttcga taccagttac ttcgatatcc gccatgtgtc ctgggacagc tatatcgagg
     1021 ttggtggtaa tcgttacagc gttcccgaag cgctgtgtgg tcagccggta tcgatacgaa
     1081 tatcgctgga tgacgagttg cggatctaca gtaatgagaa actggtggcc tcacatcgcc
     1141 tctgttcagc atcgtctggc tggcagacag tgccggagca tcacgccccg ctctggcagc
     1201 aggtcagtca ggtggaacat cgaccactga gtgcctatga ggagctgttg tgatgcatga
     1261 actggaagtc ctgctgagtc gcctgaaaat ggagcatctg agttatcacg ttgaaagcct
     1321 gctggaacag gcagctaaaa aagagctgaa ctaccgggag ttcctgtgca tggcgctaca
     1381 gcaggaatgg aacggcaggc atcagcgcgg tatggagtcc aggctgaagc aggctcgtct
     1441 gccgtgggtc aaaacgctgg agcagttcga ctttaccttc cagccgggca tcgaccgtaa
     1501 ggttgtccgg gaactggctg gtctggcgtt cgtggagcgc agcgaaaacg tgatcctgct
     1561 gggccctcct ggtgtcggaa aaactcatct ggccatagct cttggcgtga aagcggtgga
     1621 tgcgggacat cgggtactgt ttatgccact ggacagactg atcgcgacac tgatgaaagc
     1681 gaaacaggaa aaccggctgg agcgtcagct acagcaactg agttatgccc gggtgttgat
     1741 cctggatgaa ataggctatc tgccgatgaa cagagaggaa gccagtctgt tcttccggct
     1801 actgaaccgt cgatatgaaa aagcgagcat catactgacg tcaaacaaag ggttcgcaga
     1861 ctggggagaa atgttcggag atcacgtact ggcaacagcg atcctggatc ggctgctaca
     1921 tcactcaacc acgctgaata tcaaaggaga gagttaccgg ttaaaagaga aacgtaaagc
     1981 tggagtgctg accaaaaaca caacgccaat cagtgatgat gaaatggtgg aaagcggaca
     2041 gcatcagtaa cgaaagtatt tagcgggcat gaaaatggca aataacgg
//
LOCUS       DAJVQG010000280         1952 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJVQG010000280 DAJVQG010000000
VERSION     DAJVQG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.286"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            243..578
                     /gene="iraM"
                     /locus_tag="OQM83_004031"
     CDS             243..578
                     /gene="iraM"
                     /locus_tag="OQM83_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCS1522136.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(859..990)
                     /locus_tag="OQM83_004032"
     CDS             complement(859..990)
                     /locus_tag="OQM83_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HCS1522137.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1490..1566)
                     /locus_tag="OQM83_004033"
     tRNA            complement(1490..1566)
                     /locus_tag="OQM83_004033"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1530..1532),aa:Arg,seq:tct)
     gene            complement(1667..1742)
                     /locus_tag="OQM83_004034"
     tRNA            complement(1667..1742)
                     /locus_tag="OQM83_004034"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Ile,seq:cat)
     gene            complement(1845..>1952)
                     /locus_tag="OQM83_004035"
     CDS             complement(1845..>1952)
                     /locus_tag="OQM83_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS1522138.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTRLIFPESVVNSS"
BASE COUNT          647 a          481 c          338 g          486 t
ORIGIN      
        1 aaaaataatt ctttaaaata cctgacatgt aatacagaaa aaacacaaaa ccatacctta
       61 aataaaaacc tgattatcaa gcagatatgc atggataaac tacaagacga gatataaacc
      121 accctgcatt taaataaaca ataaacaaca tcagaaaaat aattctgctc tatggtttac
      181 attcaaaaat atcatttata cttttcagaa catcaccagc aaggcataaa caaggaaact
      241 aaatgaagtg gattgtgatt gatacagtta tccagccatc atgcggaata tctttttcag
      301 tcatatggag taaaataaaa ttaataatct ggtatcaatc ggatgctttc ttacctcctg
      361 aaagtatatt tacactgact cacacaggca tcatgctcaa taacaaagtg ctacctgtaa
      421 ccatttacaa cgtagtacca ttcaataaaa cattctggaa ttcaatcaaa aatagccagg
      481 aatgccctac aaatacagat aacgtattga atgaatgctt taataaccgt tgcactctgc
      541 aaatatgtcc ttatggacta aaacaacaaa gtccataagg agtttactca catctgacaa
      601 aatcaatata aacagcccct ccggagaggg gctggagagt ggcgctatgt gccattgcat
      661 ggtgccgggt gcctcccggt gaattcagta ccagcacctg aatccgcgat tatcccatat
      721 acctactcgc tgattgcccc tccgcacagg gggattcacc atgccagttt cttttaacaa
      781 actccccgca aaccagacaa cagtcaaccg cctgaattgt gaagtattta aaaatttctc
      841 ccgctaactg atacccggct aacagtctgg cgttttcttt ttcagcaacg ggaaagcaac
      901 aaccaccaca cccgccacca gcacaccgtc agccagcact gacattatcc ggctgctgca
      961 atgccattca caaaaacagt aagcaatcac tttttaccgt aacaggtgat aatccagata
     1021 tgtatctacc ccagatgagt aatccgaagt tcatccatac cacaggtcct ggctattctg
     1081 ttgtactcct gaacaagagc aaataattct gaattagcaa ccatgaactc atcgcaaacc
     1141 ctctgtatag catcactatt cagaataata acgtctcttc ccgaaagacg atcaggagta
     1201 cagaacaaaa ctgtcaaacg gctgaaggcc tttgctcgtg ctgcattgac tatatcaata
     1261 cgctgcctaa ggatgaaaca ccccgacgcc tcatcaatat tcactctacc cacaccatat
     1321 gaatgataaa tatttaatgc tgaaaaaacc attagaccgt ataacaaaca ctcaatcaat
     1381 acttaacaga acttttattt ttgacaaaca taaaatattt tcaacaatat cctgagccag
     1441 gtatatttca gtataaggct ctgccggaag gaatctggaa gaatgaatat ggcgcgctgt
     1501 actggattcg aaccagtgac cgattgctta gaaggcaatt gctctgtccg gctgagctaa
     1561 caacgctgaa taccgataat ggaccgccat cggggacccg cccccgcacc aacaaccctg
     1621 ttatcgtgtc gtctgctctt cctgataagc taatggcggt ttgtgatggt ggcccttgct
     1681 ggatttgaac cagcgacctg gcgattatga gtcgctcgct ctcaccactg agctaaaggg
     1741 ccgggagcag aataataacg gtccgtaatt aattccgcaa taaaaaaccc gctcggcggc
     1801 gggttgtaga aactcttcta acgtcaggca taaaacgccc atagttatga cgaatttacc
     1861 acagattccg gaaaaatcaa cctagttacc ttttttaact gccgctcagc ccatgcttct
     1921 tcaatatcaa accgagtcac cagcgcatca ta
//
LOCUS       DAJVQG010000281         1947 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJVQG010000281 DAJVQG010000000
VERSION     DAJVQG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.287"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            122..952
                     /locus_tag="OQM83_004036"
     CDS             122..952
                     /locus_tag="OQM83_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HCS1522139.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="OQM83_004037"
     CDS             complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="OQM83_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCS1522140.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
BASE COUNT          551 a          454 c          414 g          528 t
ORIGIN      
        1 gtgtagagtt gatatcacta caacagaaac atgcatgtca tttgtttact tctcttttta
       61 ctacagaaaa atttatctat ttttctaaac atcagtgaaa tactcataaa aggtaactat
      121 tatgtctatg ccattaagca atgcccttca gagtcaaatt attacagata atcacttcct
      181 tcatcaccca atagtcgaca gtgagtttac acataaatta aaatatgcac gcatggattt
      241 ggaaaacatc tatcttccgc ctcttactcg tggcaataac cataattacg atggaaaaag
      301 cgctgtcgaa attagaaaac ttgatattag taaagagtcc tggccattta attatgtgac
      361 aggagcatgc agagaatccg atggtattac aacaacagga cgtatgctct atcggaactt
      421 gaaaatcacc tcagctttag acgaaatata tggtggcata tgtaaaaagg cgcatgctgc
      481 cacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt tcgatcctgt
      541 tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc ctggttatgc
      601 cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa gatggaccaa
      661 tgagcagcca aggattgata tctatattga tcagataatg acggtaacag gggtagcaaa
      721 ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagttgg gtaatgaccc
      781 aataattggt atagaagcct atcccggtac cgcggagatc catgcaaaaa tgggatataa
      841 agttatcccg ggtgatgaaa atgcaccact caaacgtatg actctgcaac cgtcatctct
      901 tccagaactg tttgaattaa aaaatggtga gtggaattat attggtaaat aactaccagg
      961 aagaatgagt actaacattc aggttataca gatagctaaa caataccctg caatttacaa
     1021 agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacattta cccttgcgct
     1081 tattataata agtcaggcgt caactttatc cagcggccat gaatcaaaca gatagccatc
     1141 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg
     1201 ccgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg cgaggatctg
     1261 tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg
     1321 gatccacatt ggattgtttt cccgccactg ccaggactga cggaacagct ccaccagcat
     1381 gctgttatgc gttgcttcgg caatggcgag atggaactgc atatcaccac tttcgctgct
     1441 gcccggcgcg ctggaagcca gctcacgctc ttccagttgc aatgcctgac gcattttgac
     1501 gatatcttcg cgggtagcct gcaaagcggc aaattccgcg atgttacttt ccagtaactg
     1561 acgcgcctgt aacagctcaa aaggaccggc atcattgcag acgttagcat ccggactgtc
     1621 tgtgttgtgg ctgcctgagc tatcaagaac atagataccg gcaccccggc gcacttccac
     1681 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtcctcg tgacatcaag
     1741 catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtatg gcgtctttat
     1801 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga
     1861 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa
     1921 cgccttatcc ggcctacaac gggcaat
//
LOCUS       DAJVQG010000282         1944 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJVQG010000282 DAJVQG010000000
VERSION     DAJVQG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.288"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(1..472)
                     /locus_tag="OQM83_004038"
                     /pseudo
     CDS             complement(1..472)
                     /locus_tag="OQM83_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            complement(525..1121)
                     /locus_tag="OQM83_004039"
     CDS             complement(525..1121)
                     /locus_tag="OQM83_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1522141.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1148..1750)
                     /gene="yadL"
                     /locus_tag="OQM83_004040"
     CDS             complement(1148..1750)
                     /gene="yadL"
                     /locus_tag="OQM83_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HCS1522142.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(1765..>1944)
                     /locus_tag="OQM83_004041"
     CDS             complement(1765..>1944)
                     /locus_tag="OQM83_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1522143.1"
                     /translation="TTDDATFLKPNSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGV
                     PGNFRALATFNFIYQ"
BASE COUNT          535 a          431 c          390 g          588 t
ORIGIN      
        1 tcccaggtga actaacgtta gttacggtac cgtaagctga ccacatgttt gaaattaata
       61 atgtgtaata caaaccagga actgatgtat taaataattt atgggagcca tacattttac
      121 ctgagtcaac cattgcattt tcaatacgca ttgcgctacc acttgtgcta tcacttctgt
      181 tgcaataaag aaaaccacca ccagaagcgg tatctaactg actctactgt aattttacac
      241 ctgtaggtcc aagctgcgtc ggtttgtaga aaactaaatt atttgtactt gttgcatatc
      301 tgaatatgac agtagcatcg ccattcggcc ccgtttctct ccatttttga tctgacggac
      361 caacgccgta actattccca acaatatatc ctacgttatc atcagtgcca gcactcgcgg
      421 tataacaact caagcacaga attaaaaaaa aatgtctgaa gaatttcttc attatcactc
      481 caccatctac tgctgtaaca gtcgcagtta tttgttttga tctattattc gtagtacaca
      541 tcaactaata tatgactcat tacttggcca gatatgacat ccagcgcggg atcgattttt
      601 tgcattctgg cataaaattt ccatgaagaa ttatgcaggg cagaagtcat tactggatac
      661 acggaacgag aatttcctgc tgaactaata acattgaata tattggtcgg gtcctgaatg
      721 gaaaagagaa cgacaccgac atttttagcc ccggttgcga cttcattggc aaatatttgc
      781 cttgaacctg cagccatgac accgctaagg gctctaaaat caatatgtaa ctgattaagc
      841 acatcgccag ttccctgtaa tgcacagtct gatactgtga cagtgaactc ttttccacct
      901 ggttgatagc tttcaggagt aacattatct gaaaaatatc ccaccccaac gacgccaaga
      961 tcaatattac cctggttact gattccgctt ttgcaagtgc tgttttttac attggcaaca
     1021 aggtcaatat cctgcccagc gatggctgga taggtaatgg gaaactgtag tgacagaaac
     1081 agaattattc tcttcatcag cttacgttga gtgggatgca tatatagttc ctggatctgt
     1141 cagtctgtta gttataggtt actgtaaatg tggcaggtgc agaaaattta cccgcagtga
     1201 cattgcttac ggttttattt ttttcgacga ccagtcgagc actcatcgga taataaacaa
     1261 ccgcgttggc tgcattggag atagtgacct cttgagatgc tggtgtcaca caactaaatt
     1321 gtgttgcgct ccctgttgcc ggagtttcgg tgctccagac ttcaacggcg acggcacttg
     1381 ctttatctgc ggctgtggat ccatttgcga gccccgcacc gtcattagca gtaccttcgc
     1441 atgtggctcg cttggttaat tttatttgtg cttttttatt ggggataccc gcacagtctt
     1501 tgaatttgag tttgaatgtt ttaactttgg tcttggcatt gatttcagaa atatatacat
     1561 cgccaaagtc aacaacagaa atgtcctgat cagagtcatt tactaatgta gcggtacaag
     1621 tacctatttc tacggtagta atcacggtca gcccaatgct gtctgttgcc gcataagagg
     1681 gtacgctgaa caatgaggca gccaaaacga cactgctgga taatccataa cgtaaatttt
     1741 taaatgtcat cttaattatc cttcttattg atagatgaaa ttaaacgttg ccagtgcacg
     1801 aaaattcccc ggtacgcctt gccctgcatc tgtttcacgt agcgcaacgg tcatttcaag
     1861 acccttatcg ggctgcatct cgtctgtgct ccagcgtatc ttttccgcac tgttaggttt
     1921 aaggaaagtg gcatcatcag tagt
//
LOCUS       DAJVQG010000283         1925 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJVQG010000283 DAJVQG010000000
VERSION     DAJVQG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.289"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..564
                     /locus_tag="OQM83_004042"
     CDS             <1..564
                     /locus_tag="OQM83_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1522144.1"
                     /translation="TNNCMESVLCNESDFGINMNNVTNSSIDFTPLVNEPFVLACRRD
                     HPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLDWFYEVRHLSTSLGL
                     VEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKDAVLSPAAERFFALL
                     INLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(605..1600)
                     /gene="pgl"
                     /locus_tag="OQM83_004043"
     CDS             complement(605..1600)
                     /gene="pgl"
                     /locus_tag="OQM83_004043"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCS1522145.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIVAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
BASE COUNT          500 a          518 c          451 g          456 t
ORIGIN      
        1 acgaataatt gcatggaatc ggtgctgtgt aacgaatcag actttggcat aaacatgaac
       61 aacgtcacaa attcatccat tgattttacc ccactggtca acgagccgtt cgtgctggcc
      121 tgtcggcgtg accatccatt ggccaaaaag cagcttgtag aatggcagga gttagtgggt
      181 tacaaaatga ttggcgtacg ctcctccagc ggcaaccgac tgttgataga acaacagctg
      241 gccgacaagc cctggaagct ggactggttt tacgaagtgc gtcatctttc gacgtcatta
      301 ggactggtag aggcgggact ggggatttcg gcgcttcctg gtctggcaat gccccatgcg
      361 ccctattctt cgattattgg tattccactg gtggaacccg ttatacgtcg gacattgggg
      421 attattcgcc gcaaagatgc cgtgctttct ccggcagcag aacgcttttt tgccttgctg
      481 attaatttgt ggactgacga taaagacaat ttgtggacca atatcgtcga acgccagaga
      541 catgcgttac aggagatagg ttaatacacc ggcgcaggag agactgcgcc gggtaaatca
      601 gcggttagtg tgcgttaacc accacccaca ttggtccctg cccgaccgca tagcggcctt
      661 tttcatgcag tagcccctgc tcgccaacaa tttcgtatac cgagatgtgg tgagattttt
      721 gcccggcgac aatcagatac ttgccgctgt gatcaacatt gaagccgcgc ggctgggttt
      781 ccgttggctg gaagccttct ttactcaaca cgctgccatc ttccgaaacg ctgaaaacgg
      841 taatcagact ggcggtacgg tcgcaggcgt ataaatgacg accatccggg gtgatatgaa
      901 tatcagccgc ccaacaggtg tcggagaagt tttccggcat catatccagc gtctggacac
      961 attcaatatt gccgtgtgga tctttcagtt cccagacatc cactgagctg tttaactcat
     1021 tgacgcaata cgcatattgt tcgtttggat ggaataccat atgacgcggg ccggcccctt
     1081 caacggtagt cacttccgca gggtcctgcg ccaccagatg accatcatcg ctgaccgtaa
     1141 acaggcaaat gcgatcctgc tttaatgccg gaacccacag cgtacggttg tccggtgaga
     1201 tattggcgga atggcaaccg tccagcccct cgaccacatc gacgacgccc actggcaggc
     1261 catcttccag acgcgttacg ctcacgttac ccgcattgta agaacctaca aagacaaact
     1321 gcccctggtg atcggtggaa atatgcgtcg gactacccgg cagcgcagac tctgcggcaa
     1381 aggtcagtgc gccatcgtcc ggggcaatac gatacgccag gacgcgaaac tcagggcgaa
     1441 caccaacata gagataacgt ttgtccgggc tgaccaccat cggctgcacc tgccccggca
     1501 catcgacaac ctgtgtcagc gtcagtgcgc cttcatgatt caggttccag acgtgaattt
     1561 gctggctctc agggctggcg atataaactg tttgcttcat gaacacacct caaaaatgat
     1621 aaatccctta tctttcatac acataacaac acttactgga taaaaaacag acaacaaaac
     1681 acgataaaat acaatatcgc acatcaatta aatcaataag ttagcactcc atttcgaatg
     1741 gcatattact aaaatattag tcaccgacac gaatttgaca atgccacaga tattttcgcg
     1801 actattcagg gagtatgact ctccccgctt ttgtttacct gtatcaacga ccgtaaacac
     1861 tatgccataa caacttctgg ccaacttccc attcgctcaa caatcaactc acttccggca
     1921 caaat
//
LOCUS       DAJVQG010000284         1917 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJVQG010000284 DAJVQG010000000
VERSION     DAJVQG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.290"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..174
                     /locus_tag="OQM83_004044"
     CDS             <1..174
                     /locus_tag="OQM83_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1522146.1"
                     /translation="TTFTAGHERTLLLWLLNQLGRPRDVPVTNVVALLVMLVTTLPIL
                     GAWWLTREGDNGQ"
     gene            196..1620
                     /gene="patD"
                     /locus_tag="OQM83_004045"
     CDS             196..1620
                     /gene="patD"
                     /locus_tag="OQM83_004045"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCS1522147.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHAASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            1830..>1917
                     /locus_tag="OQM83_004046"
     CDS             1830..>1917
                     /locus_tag="OQM83_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HCS1522148.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCM"
BASE COUNT          485 a          400 c          557 g          475 t
ORIGIN      
        1 acgaccttta cggcaggtca tgaacgaacg ttactgttgt ggttgctcaa tcagcttggg
       61 cgaccgcgtg atgtaccggt aactaacgtg gtggcactgc tggttatgtt ggtaacaacc
      121 ttgccgatcc tgggggcctg gtggctaacc cgcgaaggcg acaatggtca ataaccactg
      181 atacaggaat atgctatgca acataagtta ctgattaacg gagaactggt tagcggcgaa
      241 ggggaaaaac agcctgtcta taatccggca acgggggacg ttttactgga aattgccgag
      301 gcatccgcag agcaggtcga tgctgctgtg cgcgcggcag atgcagcatt tgccgaatgg
      361 gggcaaacca cgccgaaagt gcgtgcggaa tgtctgctga aactggctga tgttatcgaa
      421 gaaaatggtc aggtttttgc cgaactggag tcccgtaatt gtggcaaacc gctgcatagt
      481 gcgttcaatg atgaaatccc ggcgattgtc gatgtttttc gctttttcgc gggtgcggcg
      541 cgctgtctga atggtctggc ggcaggtgaa tatcttgaag gtcatacttc gatgatccgt
      601 cgcgatccgt tgggggtcgt ggcttctatc gcaccgtgga attatccgct gatgatggcc
      661 gcgtggaaac ttgctccggc gctggcggca gggaactgcg tagtgcttaa accatcagaa
      721 attaccccgc tgaccgcgtt gaagttggca gagctggcga aagatatctt cccggcaggc
      781 gtgattaaca tactgtttgg cagaggcaaa acggtgggtg atccgctgac cggtcatccc
      841 aaagtgcgga tggtgtcgct gacgggctct atcgccaccg gcgagcacat catcagccat
      901 gccgcgtcgt ccattaagcg tactcatatg gaacttggtg gcaaagcgcc agtgattgtt
      961 tttgatgatg cggatattga agcagtggtc gaaggtgtac gtacatttgg ctattacaat
     1021 gctggacagg attgtactgc ggcttgtcgg atctacgcgc aaaaaggcat ttacgatacg
     1081 ctggtggaaa aactgggtgc tgcggtggca acgttaaaat ctggtgcgcc ggatgacgag
     1141 tctacggagc ttggaccttt aagctcgctg gcgcatctcg aacgcgtcag caaggcagta
     1201 gaagaggcga aagcgacagg gcacatcaaa gtgatcactg gcggtgaaaa gcgcaagggt
     1261 aatggctatt actatgcgcc gacgctgctg gctggcgcat tacaggacga tgccatcgtg
     1321 caaaaagagg tatttggtcc agtagtgagt gttacgccct tcgacaacga agaacaggtg
     1381 gtgaactggg cgaatgacag ccagtacgga cttgcatctt cggtatggac gaaagatgtg
     1441 ggcagggcgc atcgcgtcag cgcacggctg caatatggtt gtacctgggt caatacccat
     1501 ttcatgctgg taagtgaaat gccgcacggt gggcagaaac tttctggtta cggcaaggat
     1561 atgtcacttt atgggctgga ggattacacc gtcgtccgcc acgtcatggt taaacattaa
     1621 gttagatgtt agtggctgtt ataggacggc cactatcgtt ttaggttctg agtttaatgg
     1681 cggttaagta ttagaatgat tacattaata actagcgtaa aaattaggct aaatgtttga
     1741 tggcgatttt tcatcctgtc acagttgatt agcataaggc tttagagtat cgttattaaa
     1801 ctaataattt ataagtagaa gtattaacaa tgaaagttca attgtacaaa ttcacggaag
     1861 ataaaaataa aacccttacc ttcagatgga cgaaaaaaca ctttgagttt tgtatgg
//
LOCUS       DAJVQG010000285         1887 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJVQG010000285 DAJVQG010000000
VERSION     DAJVQG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.291"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..389
                     /locus_tag="OQM83_004047"
     CDS             <1..389
                     /locus_tag="OQM83_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522149.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="OQM83_004048"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="OQM83_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCS1522150.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="OQM83_004049"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="OQM83_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCS1522151.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="OQM83_004050"
     tRNA            complement(1806..1881)
                     /locus_tag="OQM83_004050"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
BASE COUNT          503 a          446 c          457 g          481 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattag
//
LOCUS       DAJVQG010000286         1872 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJVQG010000286 DAJVQG010000000
VERSION     DAJVQG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.292"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..171
                     /gene="ydcY"
                     /locus_tag="OQM83_004051"
     CDS             <1..171
                     /gene="ydcY"
                     /locus_tag="OQM83_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 domain-containing protein"
                     /protein_id="HCS1522152.1"
                     /translation="HMNELLIALSDDAELSREDRYTQQQRLRTAIAHHGRKHKEDMEA
                     RHEQLTKGGTIL"
     gene            complement(172..621)
                     /locus_tag="OQM83_004052"
     CDS             complement(172..621)
                     /locus_tag="OQM83_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1522153.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(618..1136)
                     /gene="mddA"
                     /locus_tag="OQM83_004053"
     CDS             complement(618..1136)
                     /gene="mddA"
                     /locus_tag="OQM83_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCS1522154.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1317..>1872
                     /gene="curA"
                     /locus_tag="OQM83_004054"
     CDS             1317..>1872
                     /gene="curA"
                     /locus_tag="OQM83_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCS1522155.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPSDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAE"
BASE COUNT          487 a          481 c          506 g          398 t
ORIGIN      
        1 catatgaacg aattattaat cgccctgagc gatgacgcgg agttaagtcg ggaagatcgc
       61 tacacccagc agcagcgttt gcgcaccgct attgctcatc acggtcgcaa gcataaagaa
      121 gatatggaag cgcgccacga acagttaacc aaaggcggca ccatcctctg attaaaatga
      181 gcgtctggct accaaccagg cgccaaccac aagtaaaatt gcgccacaaa ttgggccgaa
      241 caacgcccgt agtggtacgc catgactgcg gaagatatcc agcatcagac cgccaattag
      301 ctggctggcg accagtactg caatagttgt cgccgctccc acattctgat atccgctgat
      361 actggcgaag acaaaaaacg atcccagcaa gccgggtata agtgtccacc agcgcacgct
      421 ggacaccagt tcgccaaacc ccgccatgcc ttgtttaaac cataaaatag aaacaaagag
      481 cacaatcccc accagtgagt tcagcaacat ggcgatgaga atggtagagg aggtctgggt
      541 gatgcgcacc atcagcgtgt tctggactac cagaccaatt ccggcggcaa tcaaaaaagc
      601 aagggtgagc gactgattca tccaatcgcg tccggttcag tgcgttcgtc gagttgcaac
      661 tgcataaatg tcagatccag ccagcgccca aatttagtgc ccacctgcgg catttgtgcg
      721 gtgacgacaa atcccagcga atggtgcaga tgcagcgagg cctgattttg cgattcgatc
      781 ccggcgacca tgacatgctt cccgcaatcc cgcgcttcat caatcaatcg gcttaacaat
      841 ttacgcccca gacctttgcc ctgatgatcg ggatggacat aaaccgaatg ttccacggta
      901 tggcgaaaac catcgaaact acgccagtcg ccaaacgagg catatcccgt cactacgcca
      961 tcttcttcac ttaccagcac cggataacct gctatggtcc gcgcttcaaa ccaggcaatg
     1021 cggttatctg catcaaccgt ttggtcattc cagatagccg ccgtatacaa cacggcgtgg
     1081 ttataaattt ccgcaatggc agcacaatcg gctttgcggg caaaacggat ggacatgtct
     1141 ggcctcgata aaataatgat gatgatgtta tcaatactat cgaaaacagc ccgaagcaac
     1201 ggattcctcg atgaaattat tgcgtgttgc agaggggatg tgacggctgc aaacaaaacc
     1261 aatcccgtga gcctgctact attgatggat tcatgccaaa agcacaggag agcattatgg
     1321 ggcaacaaaa gcagcgtaat cgacgttggg ttctggcctc gcgtccacat ggcgcaccgg
     1381 ttccggagaa tttccgtctt gaagaagatg atgtcgccac accgggtgaa ggacaggtgt
     1441 tactgcgcac agtttatttg tccctggacc cgtatatgcg tggacgtatg agcgatgagc
     1501 catcttattc accgcctgtt gatattggcg gcgtgatggt cggcggcacg gtgagccgtg
     1561 ttgtggagtc gaatcatcct gattatcagc ctagcgactg ggtgctgggc tacagtggat
     1621 ggcaggacta tgacatatcc agtggtgatg atctggtgaa acttggcgat catccgcaaa
     1681 atccatcgtg gtcgctgggt gtgctgggga tgccaggctt taccgcttat atggggctgc
     1741 tggatatcgg tcagcctaaa gagggcgaaa cgttggtggt agctgcggcg acaggtcctg
     1801 tgggagcaac ggtggggcaa atcggcaaac ttaaaggttg cagggtggtg ggggttgccg
     1861 gtggcgcgga aa
//
LOCUS       DAJVQG010000287         1818 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJVQG010000287 DAJVQG010000000
VERSION     DAJVQG010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.293"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(8..859)
                     /gene="hchA"
                     /locus_tag="OQM83_004055"
     CDS             complement(8..859)
                     /gene="hchA"
                     /locus_tag="OQM83_004055"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCS1522156.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            1115..1310
                     /locus_tag="OQM83_004056"
                     /pseudo
     CDS             1115..1310
                     /locus_tag="OQM83_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353128.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1450..>1818)
                     /locus_tag="OQM83_004057"
     CDS             complement(1450..>1818)
                     /locus_tag="OQM83_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCS1522157.1"
                     /translation="AMMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYV
                     YSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDF
                     YDNNGIATDDVVGVGLVYQF"
BASE COUNT          478 a          370 c          445 g          525 t
ORIGIN      
        1 acagggatta atccgcataa gctgccagca tttcctgcgc cgccagttta cccaacgcat
       61 tcgctgcgaa aggactgtcg ccggtgagaa ctttacggtc cttatgtact cgcccggtga
      121 tgtcgtcatt aataatgttc atgcccattt tcttcagctc ttcgccgaag taccaggtga
      181 gatgacccgg catatagcct atctctggcg tttgtttgtc tgcggcgtct gggaatgcgc
      241 aaatggaata accattcagt gggttatcgc cgtggcgaag cgccagaaaa gccgccgggc
      301 cgtggcaaag ggagataaca aaacggtcat ttttgattgc ccactgcaaa gcagcagcta
      361 cgtcctggct ttctggtaaa ccaataagtg cgccatgacc accaggaaca aagattgctg
      421 catattcgct atcagcgttg aggctggcga caacatccgc gagtttcttc ggattgcgga
      481 acaacgattt atgctgctca aagaatggca ttactttttc atctttgtgc ggcatagccc
      541 agtattcaaa cttggtcatc agaccggaaa tggtcgccac ttcgaattcg aaaccagcag
      601 catggagatg atacaacggc agcaacgttt caatcggatg gttaccggtc gagaacagtt
      661 ttccgttatc ggttggcaaa taacgttcgt ccgcggcgat caccagaatt ttatgcttac
      721 cgcgatacgg ttttggatag tccacgccat caagatcaga gacgggactg gtatattggc
      781 taagcgaata ttctgaaggg aagaatgcat tatcttcagc aatatcgacc tgcggatttt
      841 tacttgtttg aacagtcata gtgtattcct tattgttgct tagttagggt agtcactata
      901 tttgcgactg agctggtcgc tatttttaac cagtctggaa aattgagtca atatatcgaa
      961 agcggtagtc ggcgggtgta agtatgtaat gatgatgctg cactggtgtt gaatattatt
     1021 cacgccaaat tttccttatt gagcaacgtc gtcgcaagtg gcctcaatga ttttaggcca
     1081 ttatcaaagg tacggggcgg cggggagaga tttagtgctg tgaaatattg tggtactgat
     1141 gaatatttat atcgcggatt ggtagagaaa agattatttc atgcactatt tttgcaaata
     1201 tttctatatg ggaagcgtgt ttgttacgga ttaattcata aagaaatgca tttgggtgtt
     1261 ggtttgaaga gtcgctggtt gatgttattg tgccaggagg ggcagggtaa ggtgctgtat
     1321 cgatatgcat tggcaattgc gtgtgccgca gatggagatg aaatgccaat aaaaaagcct
     1381 gaattcgatg agggacgaac tcaggctagt gtttagtttt acagtaacac gccgcagcac
     1441 gttggctgat tagaactggt agactaagcc cacaccaaca acgtcatctg ttgcgatgcc
     1501 attgttgtca tagaaatcgt catctttatc caacaagttt attttatagt caacatatgt
     1561 tgagaagttc ttattgaaat agtaggtcgc tccaatttca acatagttga ccagatcctg
     1621 atcaacatac tcctgatgac cattgttgtt atagtcgtta ccgccgaggt ctttgccttt
     1681 tgaataaaca tatgcaagag atgggcgcag accaaaatcg aactgatact gtgctacagc
     1741 ttcaaagttc tgggttttat ttgcgatacc cacattacca tatggtgtca tattgcgggt
     1801 ttcagcatac atcattgc
//
LOCUS       DAJVQG010000288         1791 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJVQG010000288 DAJVQG010000000
VERSION     DAJVQG010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.294"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            66..623
                     /locus_tag="OQM83_004058"
     CDS             66..623
                     /locus_tag="OQM83_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS1522158.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(750..920)
                     /locus_tag="OQM83_004059"
     CDS             complement(750..920)
                     /locus_tag="OQM83_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522159.1"
                     /translation="MVRLPPVVMSAKSATARTTIAREDAEDHQVKLSAQKLEELLASM
                     VKDEVDRNDGIY"
     gene            complement(1035..1304)
                     /locus_tag="OQM83_004060"
                     /pseudo
     CDS             complement(1035..1304)
                     /locus_tag="OQM83_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            1361..>1791
                     /locus_tag="OQM83_004061"
     CDS             1361..>1791
                     /locus_tag="OQM83_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS1522160.1"
                     /translation="MVKSMKTNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIR"
BASE COUNT          510 a          437 c          358 g          486 t
ORIGIN      
        1 cgtctcacca cctccctgat tggcgtcaga ggtggtgatc atccaattaa ttggagtcat
       61 cacatatgaa tacagctata actttttcta acgagtcacc aaaagtcttt ttcaaagatg
      121 gtcgcctggt taccacctct caagctgttg ccgactactt cgataaacaa cataaacacg
      181 ttttagctaa gattgacaca ctcgactgct ctcccgaatt tacatcagcc aacttttcgg
      241 ccgatgttca gactgtcgaa attggcaacg gcgcagaacg tgaatcacga tgctacctga
      301 tcacaaaaga cggcttcatg ttcctcgtaa tgggatttac tggcaaaaaa gcagccagac
      361 taaaagaagc ttacattgaa aaatttaacg caatggaaga agaactccac aaacgtcctc
      421 ctgcagcaca aaactccccc gccccaaata atggatgcgc attactgatc cacttcgata
      481 aacacggtca ggtcgagttc acagaaaaag tccccgccga tgcgatggta tgcactctgg
      541 aacggtttaa attttatctg gagcaacatg ggtggatcgt tgcccgtaaa gaacaattgg
      601 tggaaaggtt gatgcggttt taatgaatgc aaaaccccgg atgatcaccg aggttaattt
      661 tatcgagcgt gattgccgct aatcgccatc gctctgattg agcgtaaatc attcaggtta
      721 ttcagttctt ccttctgctc tcgctgacgt caataaatcc catcattgcg atcaacctca
      781 tccttaacca ttgatgcgag cagttcttcc agtttctgcg ctgacagctt tacctggtga
      841 tcttcagcat cttcccgggc tattgtggtt cgcgcagtgg ctgattttgc tgacatcacc
      901 acagggggca gacggaccat tgaaccatgg cctccggaac aaataagggc aaaaataaca
      961 accactatcg aaagctcaca aactaaccgc agcacgttcc tgcatacgac gtgtctgcgg
     1021 cataatccca atgattactc cctgacagga tttgcaggcc actcaatatc aggtgcagtt
     1081 gatgtatcaa cacgattcaa caatacccga tatttattcc atgcctccag caacgatctt
     1141 tcttcctccg ttgcgatttc cagatctaca gcatcctgca gtggcgcaat atactcacta
     1201 aattcctgga tgtagaactg tgtggtgacg gtcttccagc cattcggctc ctgctgtatc
     1261 gaagcatact aggctatttc aatatcgcta tgctgcggca gcatttaacc ccttgtaatt
     1321 catcgccata attgatttaa ttcacaaata aaactataac atggtgaaat caatgaaaac
     1381 aaacacagat gatggggcta aaatttacac accacttacc ctaaagcttt atgactggtg
     1441 ggttttggga gtatcaaatc ggcttgcatg gggatgtcct acaaaggaac accttcttcc
     1501 acactttctg gaacatttag gtaacaacca tctggatatt ggtgttggaa ctgggtttta
     1561 ccttactcac gtacctgaga gtagtctgat atctttaatg gatttgaacg aagctagcct
     1621 gaacgcggca tctacaaggg ctggggaatc aaaaattaaa cataaaatta gccatgatgt
     1681 ttttgaacct tatcccgcgg cgttacatgg tcaatttgat tccatttcca tgttttacct
     1741 tcttcactgc ctgcctggaa atatatctac aaaaagctgt gtaatacgca a
//
LOCUS       DAJVQG010000289         1788 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJVQG010000289 DAJVQG010000000
VERSION     DAJVQG010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.295"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..1692
                     /locus_tag="OQM83_004062"
     CDS             <1..1692
                     /locus_tag="OQM83_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCS1522161.1"
                     /translation="AIVDNGAHFDGDRRGTVNGVTPPAVQHLTAEVTADSGEYQVLAR
                     WDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQLALGNYSLTVRAVNAR
                     GQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDPTVQFEFWFSEKRIADI
                     RQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKSAFVEAIGRASDDAEGY
                     LNFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSNEITQTVNKKLENQSAA
                     IQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIENTPAGMQSQVLLAADRI
                     AMINPANGNTKPMFVGQGDQIFMNDVFLKCLTAPTITSGGNPPAFSLTPGGRLTAKNA
                     DISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVGKPFPRDSRAPERWPSG
                     TITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLIVKKNGAEIYNRTTLDN
                     TLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLVVVMKKATAGITIS"
BASE COUNT          455 a          411 c          527 g          395 t
ORIGIN      
        1 gccatcgtgg ataacggggc acactttgac ggtgaccggc gcggcacggt gaatggtgtc
       61 acgccgccag cggtgcagca tctgaccgcc gaagtcaccg cagacagcgg ggaatatcag
      121 gtgctggcgc gatgggacac gccgaaggtg gtgaagggtg tgagcttcct gcttcgcctg
      181 accgtggcag cggacgacgg cagtgagcgg ctggtcagca cagccaggac gacggaaacc
      241 acataccgct tcaggcagct ggcgctgggg aattacagtc tgacagtccg ggcggtaaat
      301 gcccgggggc agcagggcga tccggcgtcg gtatcgttcc ggattgcggc accggcagcg
      361 cctgtcacta ttgaactgat accggggtat tttcagataa cggcggtccc gaaactggct
      421 gtatatgacc cgacggtgca gtttgagttc tggttttcgg aaaagcggat tgcggatatc
      481 aggcaggttg aaaccagcgc ccgctatctt ggcacggcgc tgtactggat agccgccagt
      541 atcaatatca ggccgggcca tgattattat ttttacgttc gcagtgtgaa caccgttggc
      601 aaatcggcat ttgtggaggc catcggtcgg gcgagcgatg atgcagaagg ttatctgaat
      661 ttttataaag ggttgatcaa taaaacgcat ctcggcaagg agttgtggac gcagattgat
      721 aacggtcagc ttgcgccgga cctgactgaa atcaggacgt ccattacgaa tgtcagcaat
      781 gaaatcacgc aaaccgtcaa taaaaaactg gaaaatcaga gtgctgcaat ccagcagata
      841 cagaaagttc aggttgatac aaataataac ctgaacagca tgtgggctgt gaagctgcaa
      901 cagatgaagg acggacgcct ttatattgcg ggtatcggtg ccggtattga gaatacgcca
      961 gcaggaatgc agagtcaggt gctgctggcg gcagacagga ttgcgatgat taatcctgcg
     1021 aatggcaaca caaagccgat gtttgttggt cagggcgatc agatattcat gaacgacgtg
     1081 ttcctgaaat gcctgacggc tccgaccatt accagcggcg gtaatcctcc ggcattttcc
     1141 ctgacacctg gcggacggct gacggcgaaa aatgccgata tcagcggtaa cgtgaacgcg
     1201 aactccggga cgctcaacaa cgtcacgatt aataagaact gtcgggttct gggaaaattg
     1261 tccgcgaacc agattgaagg cgatctcgtt aaaacagtgg gcaaaccttt cccacgggac
     1321 tcccgggcac cggagaggtg gccatcaggg accattaccg tcagggttta tgacgatcag
     1381 ccgtttgatc ggcaaattgt tattcccgcg gtggcgtttc gcggtgctaa acatgagcgg
     1441 aagaataaca atatttattc gtcatgccgc ctgatagtga agaaaaacgg tgctgaaatt
     1501 tataaccgaa cgaccctgga taatacgctg atatatacgg gtgttattga tatgcctgcc
     1561 ggtcacggtc acatgacgct ggagttttct gtatcggcat ggctggtaaa tggctggtat
     1621 cccacagcaa gtatcagcga tttgctggtt gtggtgatga agaaagccac tgcaggcatc
     1681 acgattagct gaattttata acccagatac gggcaccaga aatggtgcct tttttattgc
     1741 agaaaagcga gaggtaatta tgcgtaaagt ttgtgcagcc attttgtc
//
LOCUS       DAJVQG010000290         1775 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJVQG010000290 DAJVQG010000000
VERSION     DAJVQG010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.296"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..264
                     /locus_tag="OQM83_004063"
     CDS             <1..264
                     /locus_tag="OQM83_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCS1522162.1"
                     /translation="AEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSG
                     IRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            434..712
                     /locus_tag="OQM83_004064"
     CDS             434..712
                     /locus_tag="OQM83_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HCS1522163.1"
                     /translation="MGKPDVTDSNLKNIIDDLYRPNAKVGSGSTADAVRYELATGEKV
                     GGRGHVLKAQDYTRALQDWLDKNPTASPGDRAAAENVLQDLQNALGGK"
     gene            712..1089
                     /locus_tag="OQM83_004065"
     CDS             712..1089
                     /locus_tag="OQM83_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522164.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
     gene            1558..>1775
                     /locus_tag="OQM83_004066"
     CDS             1558..>1775
                     /locus_tag="OQM83_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCS1522165.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDT"
BASE COUNT          588 a          259 c          428 g          500 t
ORIGIN      
        1 gcagaaaagt tcgccacagc aatccgaaac cactggcacg tggagaataa gctgcactgg
       61 cgtctggacg tggtaatgaa tgaagacgac tgcaaaataa gaagaggaaa cgccgcagaa
      121 ttattttcag ggatacggca catcgctatt aatattttaa cgaatgataa ggtattcaag
      181 gcagggttaa gacgtaagat gcgaaaagca gccatggata gaaactatct cgcgtcagtc
      241 cttgcgggga gcgggctttc gtaatctttc cctagggagt cagccgaatg ttgggaagga
      301 tttgacggat gaagagaaga actgcttggc cggcggcagt acgggaagcc cgggaggatg
      361 gggaccagaa gatgagaata gcgcaaggga tcgtatgcag catgaacggt ataaagatga
      421 attgcgacag aaaatgggaa aacccgacgt tacagattca aatttgaaaa atattataga
      481 cgatctttat cggccaaatg caaaagtagg aagtggaagc acagcagatg ctgttcgtta
      541 tgaattggcc acaggtgaaa aagttggtgg tagaggacat gtattgaaag cacaggatta
      601 tacaagggct ttacaggatt ggttggataa gaatccaaca gcatctccag gggatcgagc
      661 agcagcagaa aacgttcttc aggatttaca gaatgctttg ggaggcaagt aatgaaccaa
      721 tatattgcta gttatataga aaaaatggca tcattttcca ctgttttgtc tgagacagtc
      781 tcattagcta atgaatactg gcatccagaa acgccaccgg tggttatgtt gttctctcag
      841 gttggaaaaa cgattgtaaa aaatttttat gaaatggggg atattgaaag agaaaaaata
      901 tttaaacatg tcgaagatgg tatggtctcg cttgatgatg aattggccac agttgtagct
      961 acagggctta tagaggcaat agtcactgcg accgatagca attctttact ttggcaagat
     1021 ttaagtaaat ttctgggtga caaatcaaag tctcatgcat tagtgtggaa aaattttggt
     1081 caggagtgat attattcaag aagtattaag ctcgctattg agatcttaat gcttcttgag
     1141 attaaattaa agttaggacg aagtaattgc aggtggaaat aaatccatag tgacgtgtgg
     1201 caatggctat tagatgttat ggtgtgatgg gctttggtga tttaagagct aagttagatt
     1261 gccttatgaa aaagtatggt tatatattta atgatgtgta tcatatattt aaataaaaaa
     1321 cggttctctc ttgtcaagat agtaatagga tagactattg tgttgataaa tataattaag
     1381 agtctacttt gttgatttta gaagactttg gagcagggaa agatcatcgg aagtacactt
     1441 tttgtactaa ataattcgcg ctaaatgttc aataattgag atattcctta ttacctaaaa
     1501 ctatttttca tagcttatat atgatcaaat actccttgca taaataagga gaacaaaatg
     1561 gaacttaaaa aactgatgga acatatttct attatccccg attacagaca agcctggaaa
     1621 gtagaacata aattgtcaga tattctactg ttgactattt gtgccgtcat ttctggtgca
     1681 gagggttggg aagatataga ggattttggt gaaacacatc tcgatttttt gaagcaatat
     1741 ggtgattttg aaaatggtat tcctgttcac gatac
//
LOCUS       DAJVQG010000291         1736 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJVQG010000291 DAJVQG010000000
VERSION     DAJVQG010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.297"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(94..1581)
                     /gene="yeeO"
                     /locus_tag="OQM83_004067"
     CDS             complement(94..1581)
                     /gene="yeeO"
                     /locus_tag="OQM83_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HCS1522166.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
BASE COUNT          546 a          474 c          358 g          358 t
ORIGIN      
        1 gaacgaggcg aatattagcg atgcccaccc acaatgtcaa agcctgtttt ttaaatttga
       61 aatcgtttgc tgaaataatc tgcattttgt cgtttattcc gacacaactg gctttttttc
      121 acacttttgc ggttcggatc gagggtattt ccacagccaa cgtccagtaa ccattcgcca
      181 gtaaaacagc acggcccgca cagcccagtc ggcaaacatt cccatccaga caccaaccac
      241 accccagcca agcatgatgc ccagcacata accgacgaca acccggcaac cccacatgct
      301 caacatagaa acccacatgg cgtaacgggc atcgcgagca cctttaaatc cagcgggtag
      361 cacccatgag gcggaccaaa taggcataaa taaagcattt agccaaatca gaatcacaac
      421 gacatgttta acctgtggat cctgggtgta aaacgatgcc ataaccccgg caaagggagc
      481 cgttagccag gcgatggccg ttaatccaag agtggaaagc cagaacacat gccgcaactg
      541 aatctctgct tgcgctatct gccctacccc caaccttcgg cctgtaatga tcgtagaagc
      601 agagccgagc gcacttccgg gtaagttgat aagagccgca attgaaaacg cgataaaatt
      661 tccggcaata acactggtcc ccatcccggc aacgaacatt tgggttaata accgaccact
      721 ggtaaataac actgattcga cactcgcggg aataccaatc cccatgactt cccagataat
      781 gctaaaattc agcggtttaa aatagctctt taacgaaatc cttagcgcag gattaaaacc
      841 aatcgccagc acccacaaaa ttgcaactgc gccaatataa cgagaaatgg ttaaacccag
      901 ccctgccccg acaaatccca gtcccggcca ggagaaaagg ccgtaaatca atatgccgct
      961 aataataata ttaagaatat tcaggctacc gttaatcaat agcggtattt tcgtattccc
     1021 tgcaccacga agtgccccgc taccaataag agtgatggca gctgctggat aactgagtac
     1081 cgtcagctcc agataagtca acgccagtgc tttaacttct gtcgtggcat cacccgcgac
     1141 gaaatcaata atttgttcgc caaaatgatg aataagcgtt gccaacagta cggcaaacaa
     1201 cgtcatgatc accaatgact gccgcgtcgc caccctcgct cgtcgtcgat cccgcttacc
     1261 gagactaaat gccacaacga cagtagtacc aagatcgata gcagcaaaaa aagccataat
     1321 gaccatattg aagctgtccg ccaatcccac gccggccatc gcatcttttc ccagccagct
     1381 gaccagaaaa gtgctcagaa cccccatcaa caggacacag gcattctcca tgaagatagg
     1441 aacagcaagc ggggttatct cgcgccagaa caacactttg tagctcttgc gtttagcgtg
     1501 ccagcgagtg ccgtgaacaa cctggcgtaa agcagaggag atattcaaag ccgaccttaa
     1561 ttgcagaaag tgaaaccaca tttcaaataa tgagggagaa tcagcaaagc tgcaaagatt
     1621 ttcgccaaca aattgtctgc aaatgcaaca aactgttgat agaaacggca aacagttggg
     1681 gaatttaaaa atcgggtttg acaaaagatt tttcgccgtt aagatgtgcc tcaaca
//
LOCUS       DAJVQG010000292         1729 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJVQG010000292 DAJVQG010000000
VERSION     DAJVQG010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.298"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(24..1082)
                     /locus_tag="OQM83_004068"
     CDS             complement(24..1082)
                     /locus_tag="OQM83_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HCS1522167.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(1190..1430)
                     /locus_tag="OQM83_004069"
                     /pseudo
     CDS             complement(1190..1430)
                     /locus_tag="OQM83_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1655..>1729)
                     /locus_tag="OQM83_004070"
     CDS             complement(1655..>1729)
                     /locus_tag="OQM83_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS1522168.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT          412 a          507 c          379 g          431 t
ORIGIN      
        1 gacgcgtaat taattcttca atatcatccg ttctggctga ctaaatcctg tacttcccga
       61 accgtctgct caaaacgttc agtctccagc tcaacgccaa ttgcacgacg cccgagcgcc
      121 agtgccgctt tcactgtcga acccaacccc atgaaaaaat ctgcaatcag gtcacccgga
      181 cgactgctag cgctgattat ctgctgcagc atttctgccg gtttttcgca cggatgtttc
      241 ccgggataga actgcaccgg tttatgcgtc cacacatcgg tatacggcac ctgcaccgtc
      301 acgccaaaat accgccgcag atgtttatat tcactcagca gctccgcata ctgccggttc
      361 agtgaggtat acgtatccac cagctggtgg tggagctttt ccagttcacc acgccgatgc
      421 ttctcttctg ccacccgggc aaacagcgcc tgtaatttca gataatcgct ttcattcggc
      481 aactgccact gaccggcact gaaccagtgc gacaccatgt ttttctttcc tgtggcatct
      541 gcaatctgtt ttgccgttat ccccagggca gctcgcgcat cacgaaagta agaaatcagc
      601 ggggccatca catgctgttt cagtgcactg cccttcgccg catacccggc atctttcgga
      661 cgatacggcc cctgataatg ttccgcgaac agaatgcgct ctgtggcggg gaaatacgcc
      721 cgcaggcttt ccttgttgca tccgttccag cgtccggacg gcttcgccca gataatatgg
      781 ttcagcacac tgaagcgttc acgcatcatg atttcgatat cagatgccag gcgatgacca
      841 cagaacaggt aaagacttcc ggcaggtttc agcacccgcc agaactgcgc cagacactgg
      901 tccagccact tcaggtaatc atcgtcgccc ttccactggt tatcccagcc ctcaggcttc
      961 actttaaagt acggcgggtc cgtgactatc aggtcaacag aattttcggg taacgaccgg
     1021 ataaattcca ggcagtcggc gttgattaac tcacaactgg atatttttac agtattaagc
     1081 atggatcatt aagcctgtct ctgataggct cattctgctt ttgcgcaaag cagtgggcct
     1141 gaggtttgct tgtgaaccca acgcatgagc agatggctgg tgggtgcccc taacacccac
     1201 cagccgccca tttaccacaa ataaaaaagc cttcactgcg gaaggcgtct gtaacaaccg
     1261 aactgatagt ctgccagacc cgccataacc agctgggtca gtattaactg gcagcgttcg
     1321 cgtgaaaggt aagtattctg cgctatctcc ccgactgtcg ccggttcggt aacgcttaat
     1381 tcattaaaca ccactctggc ggtttctgtc atatcctgct gttttagcat gtcttttcct
     1441 tttccggtta acgtgacaca ccaataactc ttgtcgaaaa agccagcaag ctgaaagaca
     1501 ggtattcacc gccaccagcg cgtttactgt actgacgcga tttcagtcat aaaaaacccg
     1561 ctcggcggcg ggttgtagaa actcttctaa cgtcaggcat aaaacgccca tcgttatgac
     1621 gaatttacca cagattccgg aaaaatcaac cttgttacct agttaccttt tttaactgcc
     1681 gctcagccca tgcttcttca atatcaaacc gggtcaccag cgcatcata
//
LOCUS       DAJVQG010000293         1686 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJVQG010000293 DAJVQG010000000
VERSION     DAJVQG010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.299"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(784..1455)
                     /gene="queE"
                     /locus_tag="OQM83_004071"
     CDS             complement(784..1455)
                     /gene="queE"
                     /locus_tag="OQM83_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCS1522169.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            1594..>1686
                     /locus_tag="OQM83_004072"
     CDS             1594..>1686
                     /locus_tag="OQM83_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522170.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDN"
BASE COUNT          551 a          343 c          301 g          491 t
ORIGIN      
        1 atcgcactct atcaattttc acttaaagta tgaactggat atctttattc ataagaacta
       61 ttcatcaaca gcgtatagag gcggttatgt aaaaccactc gttagcctca aaacaaagac
      121 aaatttccag atttattact taatcaatga ataacaagct taaaaaacaa actattaacc
      181 taaagaaaaa attaacagac attaattcaa tattaaaaaa ataatagatt aaaatttctt
      241 aacgatttaa gaatcataca aataacactt tgattaaatt taattttttg tatcgataat
      301 tgtgaacctc tctggcatgg agaactattt tgaacatgag gtgttacgtg gatattttgc
      361 ttattacaag tactgctaat ataaaaactt gagaaagaga taacgggtta tatggtggtt
      421 tatccccgct ggcgcgggga acactctaaa cataacctat tattaattaa tgatttttta
      481 agccagtcac aatctaccaa ctttacagta tcacacaaac aacacatcca ttatgttaaa
      541 gagtacttaa ttcattgatt aaaaaggtaa atatttaaaa taactctata caaattaaaa
      601 tctaccaaac tttaccgcaa taattttcac tccagcgaaa aattaatgcc acagaatttg
      661 tagagaatgc taatgattag ccctggatgg gtaaaatgga ggagttttca gaggcgacaa
      721 acaatataaa tgagtagaag tctttacagg ttccacccat ttataacgct tataaatgtt
      781 taatcaggca atatttagat atttatgtgt ttgcatcgac aaacgccaat tacgcgcaat
      841 gcaggtttca atgcacaaac gtgtggcatc atccttttgg ctaatcggct gcagtgcaat
      901 gactcgcggt ttatcatcgg tcagcgttgc caatagttca tccagtgctt caatatcgcg
      961 tacgcgcccc actggatgct tgatttcgtt ggctcgctcc agtgcctgtg acaacacgtc
     1021 atagccgccg cgcatgttca gctttggcga tacggtaacc caggtattcg gtgtgcagcg
     1081 tacctcatga gtaccactgg tttcgatctg gcagctaaaa ccgttctttt cgagcagatc
     1141 agtcagtggt agcaaatcat gaatgcaagg ctcaccaccc gtaatcacca catgccgcgc
     1201 ggtgtatccc tggcgactaa tgacagccaa caagtcttca ctgctcgccg ccccccactt
     1261 atcactctct ttggtcttcg ccagaatgct gaaaagggag acttcccgat cctcaagctt
     1321 ttcccaggtg tgtttggtgt cgcaccaggc acagccaacc gggcatccct gtaaacgaat
     1381 aaaaatggcg gggacgccgg taaagtaacc ctcaccttgc agggtctgga acatctcgtt
     1441 aatcgggtac tgcatagcat tctctgtgaa gtggataatt gttaattatt gcagatcctg
     1501 ccacaacaat catgtcttat taacattctg ttacaggcag atttaaagaa aaatgcgaaa
     1561 catatcgtta ataattaaag ggagtaacgt attatgtcag aagaaaataa agaaaatgga
     1621 tttaatcatg tcaaaacatt caccaaaatt atatttattt tttctgtatt agtttttaat
     1681 gataac
//
LOCUS       DAJVQG010000294         1615 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJVQG010000294 DAJVQG010000000
VERSION     DAJVQG010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.300"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..239)
                     /locus_tag="OQM83_004073"
     CDS             complement(<1..239)
                     /locus_tag="OQM83_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HCS1522171.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEA"
     gene            complement(244..942)
                     /locus_tag="OQM83_004074"
     CDS             complement(244..942)
                     /locus_tag="OQM83_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCS1522172.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            complement(952..1281)
                     /locus_tag="OQM83_004075"
     CDS             complement(952..1281)
                     /locus_tag="OQM83_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1522173.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVKIRVRKGEHPFLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            complement(1281..>1615)
                     /locus_tag="OQM83_004076"
     CDS             complement(1281..>1615)
                     /locus_tag="OQM83_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1522174.1"
                     /translation="DLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKL
                     GGVADTGGSGMVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDG
                     GRLSGAYR"
BASE COUNT          346 a          539 c          367 g          363 t
ORIGIN      
        1 gcctcactca gccagggcag accaccggga tgactgtgga ccagcgccac aatctcaccc
       61 tgcatttgtg cccgcagcca gtcctccgga gccatccgga accgttcctc cggctcaccg
      121 gagatattct cgctgggaag atacctgtct ccctccggcg ttcttaccac gaagccacac
      181 gactccgctg gcgcacatcg ccgggcgtgc gccagaatcg ctgattctgt ctgtgtcatg
      241 gatttactga gaaagtttat tgatggaaag gaaaccgccg aaattaccgg tattgttgcg
      301 caactcacac ccgcgcatac acttgctgca tttgtccttc cggatatccg tcgttggttt
      361 gtcgaactcg tcagccactg ccctgcccgt gtaaccacac tcatcagaac ggtaagtcca
      421 catacaggta ttcgccagca tgatacgccc cgggaaaacc gcgccatccg tctccgttgg
      481 tgtggccagc acaaaagagg cactgaccgc gctcagttcg ctgcactgct cgatgcgcca
      541 gcggctgatc acctcctgtt ccggatcggc gtcgctgttt ccgttgacga agttcaccgc
      601 atccagaaaa cgggcgtaaa ccttacgcct gaccaccgtt ccgccgacca gactctgcag
      661 gtcttccgcc atcccggtga ccattccgtg caggttagaa accgtcagtg tcggacgggc
      721 agcactgccc ttgccgttca gttcaaatcc cgtcccctga atggggtatg cctgatactg
      781 ccgcccctgc caggtaaccg gctcaccttt ttcgttctgc tcattacaga aaaaataacg
      841 ttcaccaccg acctctgtca gatcgatttc ccagagcacc acctgggctg actgagtgag
      901 gcgtgtcgtc tcatgatgtg tttcctgtgg aatatcctgc atcagagcct cctatgccac
      961 gacctgttca aaatctgccg ttatggttac ccacaacgcc cccacgcttg ccgaccattt
     1021 acgacaaacc accctgatcg gcttccagtc ataaggtggc gtccactgaa atgcgcggac
     1081 gccaccgtgc cgttccagaa aggcttttaa aaatgggtgt tcacctttac gaacacgtat
     1141 cttcacgctg taagtcgaca actggttatt cagtcccgcc gcacgacgct gttcataacc
     1201 atcgcccagc ttcactgtca ccactttcgg ctctgatacc acattcatat ccgggcgcac
     1261 tttccagtga aacgtctcca ttaccggtat gctccactta accggccacc atcacgggcc
     1321 tgctgttgca taaagtccgc ggccgctttt ttcccgaggt cataaaccac cttcagggca
     1381 gccggaccta tctgcccgtt cgtgccatcg ttattgatct caatgttgta ctgcggggca
     1441 aacatcacca tgcctgaacc accagtatcc gccacacccc ccagcttacc atcagcaccg
     1501 cgacgcagtg gcagaatggc ttcaggtccc gcttccccca tcacacccgc gccttttgca
     1561 aaagcaaaaa acgtcggacg gttaaccacc gtgccactgt agcgactcaa atcag
//
LOCUS       DAJVQG010000295         1452 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJVQG010000295 DAJVQG010000000
VERSION     DAJVQG010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.301"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(69..521)
                     /locus_tag="OQM83_004077"
     CDS             complement(69..521)
                     /locus_tag="OQM83_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCS1522175.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(574..>1452)
                     /gene="adeQ"
                     /locus_tag="OQM83_004078"
                     /gene_synonym="yicO"
     CDS             complement(574..>1452)
                     /gene="adeQ"
                     /locus_tag="OQM83_004078"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCS1522176.1"
                     /translation="IGLFIALMGLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFII
                     TVLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLEL
                     AGIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTS
                     SVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLM
                     TSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLC
                     VVVVASLFALKIILVD"
BASE COUNT          444 a          399 c          318 g          291 t
ORIGIN      
        1 tgcggcgtga acgtcttatc cacccgacag gtcaggcgcg gtctgtaggc tgataagacg
       61 aagcggtttt aatgcagcca gtgaacgccc tcggcaggct gaaagaacgc gttatagcgc
      121 atctgaaaag cattaatatc ctgcatttca ggttcggttt cgcgcagaaa acccagcgcc
      181 agcctgacct gagcatcggt aatcggcgcg gaaaggcggg ctttttgtaa aatccgatgc
      241 cattgcagca ggttctgttc gctaccatcg acaatgacaa tttgccagat gagcaacacc
      301 tgcgcggcgt tttgcaggtg cgactcatcc gggcgatgca aataacgtaa aaattcgtca
      361 ccggctgact ttcccatttg atcgaccatc gactccacgg gtacgacatc gatattcagc
      421 cgatcactga gacggcgaat cgcttttctg gccccggatg tcagcacccg aaaaccaacc
      481 acaaacacta ccgccagcgt ggcgagcatt atccagatca tgagttctcc tgcgttatca
      541 gaactcatga tatctggaaa ccctcgccag ttactaatcc accagaataa tcttcagtgc
      601 aaacagagat gcgaccacca ccacacacag gttcagatcg cgccagcgcc cggtgcacac
      661 tttcatgatg cagtacgaca taaagccgag tgcaatccct tcggtgatcg agaaagtaaa
      721 gggcatcatc accgtggtaa taaacgcagg caccgattcg gtaaaatcat cccagttaac
      781 gcgcgccagg ctcgaagtca tcagcacgcc aacaaagatt aacgctccgg cggttgcgta
      841 aggaggaacc atcgccacca gcggggagaa gaacataacc aacaggaaca taacgccaac
      901 cacaaccgca gtcagccccg tgcggccacc gactgccaca ccagaagtac tttcaatata
      961 ggcagtaaca gacgaggtgc cgataaacgc acccgccacc gaactgacgc tatcaacata
     1021 cagcgcctta ttcatattgg ggaatttacc gttaccatct attaagcccg ctttatcagt
     1081 tacaccaatt aatgttcctg atgaatcaaa taggttgatc agcataaagg agaaaatgat
     1141 accggcgagt tcaagtgtta acgcgccgct caaatctact tcaccaatga cgccgctaat
     1201 atcaggcgga atggaataga cgccgctaaa atgaacatca ccgaaaaata atccacagca
     1261 agacgtcacc acaatagaaa ccagcaccgc ggcatgaaaa tgacgtgatg acaacacggt
     1321 tataataaaa aaacctaaaa tacctaacaa cacgccgtga gaacttaaat cgccaatcat
     1381 caccagcgtg tctttattgg cgacaataac gccagtattt tttaatccca ttaaggcgat
     1441 aaataatcca at
//
LOCUS       DAJVQG010000296         1446 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJVQG010000296 DAJVQG010000000
VERSION     DAJVQG010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.302"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..956
                     /gene="tssI"
                     /locus_tag="OQM83_004079"
     CDS             <1..956
                     /gene="tssI"
                     /locus_tag="OQM83_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCS1522177.1"
                     /translation="YNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDP
                     DQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHVQ
                     KNMNTEVLNNRTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNLVETVGSN
                     QIIKVGSNQVEKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYS
                     VNVGNNVTFSVGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLE
                     GESDVNGDAPVINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            1032..>1446
                     /locus_tag="OQM83_004080"
     CDS             1032..>1446
                     /locus_tag="OQM83_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCS1522178.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGI
                     TYANPVNPVLGAKVLPGETDLALPCPLPFILFRAYSSYRTRTPAPVGVFGPGWKAPFD
                     IRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSE"
BASE COUNT          368 a          349 c          445 g          284 t
ORIGIN      
        1 gctataaccc gtcaaaccag gacagttcat gctggatccg tgtggcacag gcgtgggcag
       61 gcaccggatt cggtaacctt gcgataccgc gcgtgggtca ggaggtcatt gtggacttcc
      121 tcaacggcga tccggaccag ccgattatta tggggcgtac ctaccaccag gaaaaccgca
      181 cacccggtag cctgccgggg acgaagacgc agatgaccat tcgttcgaaa acctataagg
      241 gcagcgggtt taatgaactg aagtttgacg atgcgacagg gaaagaacag gtctacatcc
      301 acgtgcagaa gaacatgaac accgaggtgc tgaataaccg caccacggat gtgataaaca
      361 accatgccga aaaaataggt aacaaccagg cgatcaccgt taccaataac cagatccaga
      421 acattggcgt taatcagata cagacggttg gtgtcaacct ggtggaaacg gtgggcagta
      481 accagattat caaagtggga tcaaaccagg ttgaaaaggt ggggatcatt cgtgcgctga
      541 cggtgggtgt ggcttaccag acgacggtag gcggcattat gaatacctcg gtggcgttgt
      601 tgcagtcctc acaggtaggg ctgcataaat cactgatggt gggaatgggc tacagcgtca
      661 atgtagggaa taacgtcacc ttctcggtgg gcaagacgat gaaggaaaac accggacaaa
      721 cagcagttta ttctgccggt gagcatcttg aactctgctg tggtaaggca aggctggtgc
      781 tgacgaagga cggaagcata tttctcaacg gtacgcacat tcatctggaa ggggagtcgg
      841 atgtgaacgg tgatgcgcca gtgattaact ggaactgtgg tgccacacaa cctgtaccgg
      901 atgcgcctgt gccgaaagat ttaccccccg gaatgccgga tatgcggcaa ttttgagtca
      961 ggtgcaaaaa attaaaacgg aactggtgca gtgaccagat gaaccatatt aaaagcagaa
     1021 ggaattgatt catgggcggc ggaaaaccgg cggcgcggca gggcgacatg acccgcaagg
     1081 gactggatat tgtgcagggt tcagcgggtg tgctgatagg tgcgccgacg ggagtggcct
     1141 gttcggtgtg tccgggaggg attacctatg ctaacccggt gaacccggtg ctgggtgcga
     1201 aggtgcttcc gggcgagacg gaccttgcgc tgccctgccc gctgccgttt attcttttcc
     1261 gcgcctacag cagctaccgg accagaacac ccgcgccggt gggggtgttt ggtcccggct
     1321 ggaaagcgcc cttcgatatc cgcttacaga tacgcgatga aggcctgata ctcaacgaca
     1381 gcggcggcag gagcattcac tttgagccgc tgtttcccgg cgagataagc tacagccgca
     1441 gcgagt
//
LOCUS       DAJVQG010000297         1410 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJVQG010000297 DAJVQG010000000
VERSION     DAJVQG010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.303"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            211..1407
                     /locus_tag="OQM83_004081"
     CDS             211..1407
                     /locus_tag="OQM83_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCS1522179.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYP
                     VLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGN
                     KALKRALFLSAFAALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTF
                     YTPQGS"
BASE COUNT          297 a          438 c          377 g          298 t
ORIGIN      
        1 ccttataatg gggtgttagc cagccagacc cggcatgatt actgccccca gtcgtccatg
       61 atccgggggg tgatgtcacc gggtctggtg gggcgctggt aaccgctaat aggggtcagg
      121 tcaggcactt ttgccgggac cgtctgtaac gtggatgccg gtacctgctc cccgtggtta
      181 tctggttaac ccatatacaa gggagacaga atgaccgaat ccagcgatta cgaatccgtc
      241 caggtcttta tcggcgttga tgtcggtaaa gatacgcatc acgctgttgc cattaatcgt
      301 tcaggtaaac gcctgttcga taaagcatta cccaacgacg aaaacaaact caggtcgcta
      361 atatctgacc tgaaacaaca tggtcagata ctgctggttg ttgatcagcc agctaccatc
      421 ggtgcgttac ctgtcgccgt tgcccgctca gaaggagtcc ttgtcggata cctccctgga
      481 ctggccatgc gccgcatagc cgacttacac gccggtgaag ctaaaactga tgctcgtgac
      541 gctgccatca ttgccgaagc tgcccgtacc ctgcctcacg cgctacgcac gctgaaactg
      601 gctgacgagc aaatcgccga actctccatg ctctgcggct tcgatgatga tcttgccgca
      661 cagacaacgc aggccagcaa ccgtatccgc ggccttctga cccagataca tccggcactg
      721 gagcgcgttc tcggtccgag acttgagcac ccggcggtac tcgatcttct ccagcgatat
      781 ccctcaccag aaaaactcgc ttcgctgggt gagaagaagc tggcagccca gctctgcaaa
      841 cttgcgcctc gtctgggtaa acgccttgca gcagacatag ctcaggcact ggccgaacaa
      901 accgtcgtcg ttcccggcac gaatgccgct gccgtagtac tgccacgtct ggcactccag
      961 ctcatcacgc tgcgtaagca aagagacgag gtggcgcttg aggtagaaca gcgagttctt
     1021 gctcaccctc tttacccggt cctgaccagt atgcccggag tcggtgtcag gaccgcagcc
     1081 agactcctca ccgaggtcgc ctgccgcgcc ttcgcctctg ccgcacatct cgctgcttat
     1141 gctggccttg cgccggtaac tcggcgatcc ggctcgtcaa tacgcggtga gcatccctcg
     1201 cgacggggta ataaagctct caaacgggcg ttgttcctgt cggccttcgc cgcgctcagg
     1261 gatccgctct ccagggctta ctacacccgc aaaatgagtc agggaaaacg acacaatcag
     1321 gcgcttatcg ccctggcgag acgacgctgc gacgttctgt tcgccatgat gcgcgacggg
     1381 actttttata ccccgcaggg gtcataacat
//
LOCUS       DAJVQG010000298         1408 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJVQG010000298 DAJVQG010000000
VERSION     DAJVQG010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.304"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(121..330)
                     /locus_tag="OQM83_004082"
     CDS             complement(121..330)
                     /locus_tag="OQM83_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522180.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(353..1063)
                     /locus_tag="OQM83_004083"
     CDS             complement(353..1063)
                     /locus_tag="OQM83_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1522181.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
BASE COUNT          428 a          284 c          236 g          460 t
ORIGIN      
        1 attctatttt tcagtgtggg tattagcttt acagatttca cgtagcacac atacaaaaaa
       61 agggagagct accgatgtca ctcgataatc tctccccgta tttacccaac caattctttt
      121 ttaatgtcgg acaagataat gtaattccgt ttcacctatg atttgagcat cagaaggaat
      181 acggtcaata caccactttg gaatcttgcg tgtttttggt ttgtgtgagg tgataacttg
      241 acgtttgttt ttaattctta aggctggctt agtttgtaac tttaagtttt cacaaacaga
      301 ccgaatagtc tctaaacgtt cttcaaacat taatcacctc tttaattcat aatcaaacag
      361 ccttgctata cttctgagaa atcttcgtta actgctcaat atcagccatc aaagcaatct
      421 tcttctgcga ctcatattca ctaacaagtt ctttcatagt atcaagttct tttttaagct
      481 tttgctttaa cttatccagt ttaccggaat aatgcttagt caaattagct tcagctaact
      541 gaagaatctc ttcatctgtt ttatcagtca tcagaataaa ttgaccagaa ccccttaatg
      601 cttgtctgac atcaattaac cggaaccctt cagggatact ttgaagaact ttaggaacgt
      661 cttcaatcgc tgtaccaaaa ccatcattaa agtttccttc ataatagata aacgggtttg
      721 acttaatctc gtaagcaata tcatattctg aaggtttcga ggtttcttcg aagttcttaa
      781 cagcatcgta agcgacatca agtctaagac gctgtgattt taaagctggg taatattcac
      841 gctcaagctc aaatagtgct tcagttttgc agatatcagg catttcttga ggctcaggag
      901 acacaaagga aattaccttt ggtgacatct ttagctcttg ctcaagctca atgcgcttgt
      961 catgctcttg acgctgaata gtatgtagcg gatgttcacc ctgtggtgac cagtctccga
     1021 agctatcaac ctttttaccg ttaatatata aatcgtttgt cattataatt tcctctaagt
     1081 gtggttattt cttaatagag caccttcaac gccaatacac ttagaagatg acttaacgat
     1141 ttattaaaga tgctctatta agaaaagccc taaacataat ctgtgaaggg ctttggaggt
     1201 ttgcttataa cgttaacgaa gtttcaggaa aactttcttt aaaagctctg tttaaaaact
     1261 cttaaagaaa gccctaactc atgattaggg caaagctgta aaggattcaa taatgaatga
     1321 acaaagactt tttgagatag cctctcagac caacctgaaa ggctaggacg aaaagcattg
     1381 cacctttcca actcagaata tccattca
//
LOCUS       DAJVQG010000299         1408 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJVQG010000299 DAJVQG010000000
VERSION     DAJVQG010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.305"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..386)
                     /locus_tag="OQM83_004084"
     CDS             complement(<1..386)
                     /locus_tag="OQM83_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCS1522182.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKG"
     gene            complement(447..1049)
                     /locus_tag="OQM83_004085"
     CDS             complement(447..1049)
                     /locus_tag="OQM83_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCS1522183.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(986..>1408)
                     /locus_tag="OQM83_004086"
     CDS             complement(986..>1408)
                     /locus_tag="OQM83_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCS1522184.1"
                     /translation="HLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQN
                     LYLDNMEVTGFYRVALTEAQPGDVLLCCFGSSVPNHAAIYCGDGGLLHHIPEQLSKRE
                     RYTDKWQRRTHSLWRHRAWRASAFTGIYNDLVAASTFV"
BASE COUNT          313 a          463 c          335 g          297 t
ORIGIN      
        1 tcaccctttg aggtggtttc caccagtgcc tgcacaccga aggtcaggcg cagacggtcg
       61 atgtttgccg acgtgatggt ccgggtgatc ggcgtgtcat atttcacttc cgtacccagc
      121 accgtctcgg agccggagga ttcaaaaccc tccggcggtg tctgctcctg ctcacctgca
      181 cggaacacca ccgtaacacc ggaaaagttt gtcttcccct cgctgtccag gaccggcgta
      241 ccgttcagca gcacgctttt taatccatcc accggaccgt caaccggccc ttcgctgatg
      301 gcatcaatca cactcagcaa ctgcgtggat ttcaggttgt ccttcgcttc gcgcggggta
      361 tgccccttac tgctgccttt acccattcct cacgctccat aaacgacaaa accgcccgca
      421 ggcggtttca cataaaacat tttgcatcag cgaccaatca ccacaacctg accaccatcc
      481 ccttcgtctg ccgtgctgat ctcctgagaa accacacgtg accccacgcg catttcaccg
      541 tacagaacgg gcagaacatt gccctgggca accatgttat ccagtgacga gaaatacgtg
      601 ttctgcttac cgttatccgt tgtctgtgta cggggagttc tggctttcgg tgccagcatc
      661 tgtgcaacac cgccaagcgt catactggca ccgagagaaa acagcagatt actcgccata
      721 attcctaccc ccggcatcca tatagcaacc gccataacag ccgcccccag caccgcctga
      781 aacacaccgc cacttttagc tcccaccaga cgcggtacta tgtggatcac tgcaccattt
      841 gccagcggct cattaagacg ggcagacaat tcggtttcac ctgcatcacg cccggcaatg
      901 cgcacctgat accagccgtc actcagtttc tgacgaaacg ccgggagctg tgtggccagt
      961 gcccggatgg cttcagcccc cgttttcaca cgaaggtcga tgcggcgacc aaatcgttgt
     1021 aaatccccgt aaaggcagat gcgcgccatg cccggtgacg ccagagggag tgtgtgcgtc
     1081 gctgccattt gtcggtgtac ctctctcgtt tgctcagttg ttcaggaata tggtgcagca
     1141 gcccgccgtc accgcagtaa atggcggcat gattcggcac cgatgaacca aaacagcaca
     1201 gcagcacatc gcccggctgt gcctctgtca gtgcgacacg gtaaaaaccc gtcacctcca
     1261 tattgtcaag atagagattc tgaccgttac gccaccagtc atccccgcga tgaaaatccg
     1321 gcatctcaat ccctgccaga tgataagcat cccggaacag cgtgtaacag tccgtcaccc
     1381 cgtgctcaaa gcgccgcccg gtgagatg
//
LOCUS       DAJVQG010000300         1406 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJVQG010000300 DAJVQG010000000
VERSION     DAJVQG010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.306"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            132..1151
                     /locus_tag="OQM83_004087"
     CDS             132..1151
                     /locus_tag="OQM83_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HCS1522185.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
BASE COUNT          405 a          314 c          391 g          296 t
ORIGIN      
        1 cacacaatta aacacggagc agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
       61 ctcgcagatt tccggcatca tagtgcccaa tacgggatgc ataaagctcc ctcagactgg
      121 agactccaca aatgaaatat acaccggttg gcgttgatat cgcaaaacat gtcattcaga
      181 ttcacttcat caatgagcac acaggtgaag tggttgataa acagttgcgt agacaggatt
      241 ttctgacgtt cttcggcaac cgtgagccat gcctgattgg tatggaggcc tgtggaggtt
      301 ctcagcactg ggcacgggaa ctgacaaaac ttggtcataa agtccggttg ttgcaggccc
      361 gcttcgttaa ggcattcgtc atgggcaata agaatgatgt gatggatgcc cgggctatct
      421 ggatggcggt tcagcagccg ggtaaagaaa tcgccgtaaa aacagaagaa cagcagtcgg
      481 tactggttct gcaccgtacc cgcatgcaac tggtgaagtt ccggaccgca caaattaatg
      541 ccctgcacgg gacgttactg gagtttggtg aaaccatcca caaaggccgg gcagcgatgg
      601 agcgggagtt ccccgaagca ctggaacgga tgaaagagag actgccaccg tatctcatta
      661 tggttctgga aaaccagtac aaccgactga atgagctgga ctcactgata gaggatattg
      721 aaaaacagct taccagcgtg gcgaggcaga atgaaacctg taagcggttg ctggatattc
      781 ctggcgttgg accacttatt gcgacggcag cggtggccac catgggggaa gcatcagcgt
      841 ttaaatcggg gcgagagttc gccgcatatg ttggtctggt tccaaaacaa actggctccg
      901 gagggaaagt acgtctgctg gggataagca aacgtggtga cacttatctc aggacattat
      961 ttatccacgg tgcaagagcg gtggcattag tagctaaaga gcctggcccg tggataaccg
     1021 aactgaaaaa acgtcgtcca gccagtgtgg caatcgtcgc catggcaaac aagctggcac
     1081 gaacagtatg ggcgataacc gcccatgacc gtaagtatga caggaaccac gtcagtatca
     1141 gaccatatta atcgctgata ccattaaaca atgaactctt aacaaaaggg tgaatgctga
     1201 aaggttgcta tggcggccag agtgatgaca aagacaggta agaccgtgac tcactaaacc
     1261 tgaacagtat tttgggcttg aagtccgccg tgaaaataag gggtgagtcg gcgaattaca
     1321 taggggctcg cagcgttacg gctgcaataa agccggatat aaagctgcaa cctacccgtc
     1381 atgtcaaaac aatggatgcc ttgcaa
//
LOCUS       DAJVQG010000301         1397 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJVQG010000301 DAJVQG010000000
VERSION     DAJVQG010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.307"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..481)
                     /locus_tag="OQM83_004088"
     CDS             complement(<1..481)
                     /locus_tag="OQM83_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCS1522186.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     "
     gene            complement(683..1141)
                     /locus_tag="OQM83_004089"
     CDS             complement(683..1141)
                     /locus_tag="OQM83_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCS1522187.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1138..>1397)
                     /gene="rrrD"
                     /locus_tag="OQM83_004090"
     CDS             complement(1138..>1397)
                     /gene="rrrD"
                     /locus_tag="OQM83_004090"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HCS1522188.1"
                     /translation="TVARQINPYIKVDIPETTRCALYSFVYNVGAGNFRTSTLLRKIN
                     QGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVCLWGKQ"
BASE COUNT          321 a          358 c          335 g          383 t
ORIGIN      
        1 ggatcgtaat ctgcagctgg ttagccaggg agttaatctc agcgaccaac actggcttcg
       61 tatagcgcca tgctgccagc ccttgtccgc agaagctcgc catatctttc ttctggtcaa
      121 attcatgaca tttcatgttg agctgcgcac ttaagctgtt gcgatgctga agttctccgg
      181 tgaagtagtc atcgaggact ttataggccg cgtacttgaa cccggggttt aaccaagccg
      241 cataatcgta agcaacaaac ttcccgccat atgttccacc gtgtacaccg cgctcagtaa
      301 aaaccacaga ttcgtggttt ttctccagct cggctaagaa ctctttggtc tgcttgtttc
      361 gcaggtagtg gtaaggcgat tcagattcac ttttaccact ggctttccac atatcagtga
      421 ggcagatcat gccatcttca ccgatacgaa ttggttgatt gaagagggtt aatgatttca
      481 tagcgtgtac ctactctttg aaatgaacct ttgccgcaca ggaaaccagc ccaccgaggc
      541 tcgccagcac taactggtat cctcaaaggc ccattccaaa ggggcaggtt cggtgtaaaa
      601 aacatgcgtt gcggtacgca tttattgcaa aaagccccgc atcgcgaggc tcattaaatg
      661 gactttgtga tttgcaaaaa aattatttca ggcattgcgt cctgatgtat tcctgcaggt
      721 agttaacctg cgcggttatc ttgtcgattc cacttcggag acggtaataa ttgagttcag
      781 catctgctgt aagtcctggg ctttctccat cgcccatgcc gctggctccg gtcgttgact
      841 ttgcacaggt ggcggcgact tgcaggcgct tacgcccagc agaaacatca gcacggagac
      901 tttcgatagt cgcgttagca tcagcaagct cctttatgta tcttgcgtcg agttctgcta
      961 catcacgttg acgcttccgc atgtcagcga tggtggcgtt cgccttctcc agttcactgg
     1021 ccttgttatc gcgctgctct ttgtaggcga ttgcgttatc acggtaatga ttaacagcca
     1081 atgacaggca gacgatgatg cagataacca gagcggagat aatcgcggtt actctgctca
     1141 ttgtttcccc cacaaacaga cttcacgctc aatctcacgg cgagtcatca gtcctttcca
     1201 ttgattaccg ccagcgtatg tccagcgccg tagctgatca catgcgcctt taatatcacc
     1261 ctggtttatt ttgcgaagaa gcgtcgatgt tctgaaattg ccagcaccca cgttgtagac
     1321 gaacgagtaa agagcgcagc gcgttgtttc cggtatatcg actttgatgt acgggttaat
     1381 ttgtctggcg acagtgg
//
LOCUS       DAJVQG010000302         1382 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJVQG010000302 DAJVQG010000000
VERSION     DAJVQG010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.308"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..304)
                     /locus_tag="OQM83_004091"
     CDS             complement(<1..304)
                     /locus_tag="OQM83_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522189.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
     gene            complement(1125..1337)
                     /locus_tag="OQM83_004092"
     CDS             complement(1125..1337)
                     /locus_tag="OQM83_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HCS1522190.1"
                     /translation="MLCFLIYITLPFSQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININANKNIRQVC"
BASE COUNT          449 a          288 c          261 g          384 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 ccgtcaggta ggccattttg tccagcacct gcgcaaattc aagggagaga aactgctggc
      181 tgaccagaga gagatcgagg gaaaagagcg aagagagtga ctggttcaaa tgaaaggaaa
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag tgtgaaacgt aatccggttg
      301 acatagcaac ctccgtgaaa atgaataata atccgcacat attgcatgta caacatgtcg
      361 ggcggatgag ataagcgagt cgacatctgg cgttaccggg gaatatcaga aaaatgtcat
      421 ggttatgtga tgatttagtc tgggatttta cttcagagaa acgggtgtct gtttagtcaa
      481 caaaataatc acaaacgcaa cggatgggtt ctcgacgtta ttcacgacag gctcgtacat
      541 tacagcgagt aataaaaaac ctgatatgca aagtcaaacc aatattcata atgtcttttt
      601 attcacaaca ataaataatt caataacaat cattgaaatg aatgatggtt tgtataagga
      661 atatacgcta ccgcccaccc tgataacatg atctgcacat tgccatctta acgtcgttaa
      721 acactggctt aatagtggat atttcaaatt aggaatttat taaattcacc tgatgaatat
      781 aataagcaaa tagcatagaa aataatggca actccccccg agaggaccat ttccaaagca
      841 ctgcaccacc acctggtgcc cggatgatct tgcttcagtg gtaagagtac ctgattcatt
      901 gttcccaaaa tgatgttttc cccggccccg ccaataatgt gcagctctca cgtgataatc
      961 tattgattag gttatattaa gaatcaatat ttaaaataca tccattttct tgaataaatc
     1021 aacatattta tttatcaatt gctgttttag ctctgactca tcaagctctc catatttatc
     1081 ctatagataa ttgcgaataa atacagagaa cgccatttat ccttttagca tacctgccgg
     1141 atatttttat ttgcattaat attaattgca tctccaactc cgagcatatc catcattaaa
     1201 aactttttga gattatagaa tacctgtgat aacaagtgaa acatctgcac aatgtgaata
     1261 gttagtttct tttcagagga aatgaaatag acaagctgac tgaaggggag agtgatataa
     1321 atcaaaaaac ataacatgaa tttaattaat tacgcccatc attgtggata aagttactga
     1381 at
//
LOCUS       DAJVQG010000303         1270 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJVQG010000303 DAJVQG010000000
VERSION     DAJVQG010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.309"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..>1270)
                     /locus_tag="OQM83_004093"
     CDS             complement(<1..>1270)
                     /locus_tag="OQM83_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HCS1522191.1"
                     /translation="LGIEGSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLS
                     HYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMF
                     ATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKKRWQASFGLEERAAEKTPVRQ
                     RIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSM
                     PAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQF
                     DGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQ
                     AAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVP
                     LYINSQGVAKLLRNETLTSLPKNLEPVFYNA"
BASE COUNT          299 a          340 c          307 g          324 t
ORIGIN      
        1 cggcgttata aaaaaccggt tcgagattct tcggcagact ggtcaacgtt tcgttacgca
       61 gcagtttcgc cacgccttgt gaattgatat agagcggaac gatgccatca gtgggtatta
      121 catccaccat tgccgggcgg tttttattca gtgtttgcag ggcgttatta accagcgtgt
      181 catcgaggga gaaaagcagc gttttgtttt gcatcgccag cgacacgcgg aaaaaataat
      241 ccgacattaa ttgatcgggt tgcgccgcct gcgctttcgg atactgaccg tatcgggaac
      301 tcacttcgcg acgccagatt tgcgcttcgc cctgctgagt ctggcttatc ggcaaaacac
      361 cttctggcgc tttgctttcg tgcgcaccaa tattttgcgt aaacagtttc cctggcaatt
      421 gcgcctgttc ggcagtgcca tcaaactgac cgacaaacag cggggtttgc aattttgagt
      481 cttcatacca gcacagcccc gcggcaccgt ctaacgcccc attcagcttg tcgttttcct
      541 ggctgatgtg cgaaagcatc tcttcggcaa taccgtgtga atacggcacc accacacaga
      601 agctggcccc ggcaggcata ctgttccaga ccggcgtaaa atcgaaactg gtatctacgc
      661 tggcggattc atcatttaac gccacaaaac tgtgccagcc gtcgttaccc atttcgaagc
      721 gtacgccagc aaaagaaggc attaaccgct ggtagccaaa acccagcaac ctggcgctga
      781 ccacgatgcg ctggcgtact ggcgtttttt cagcagcacg ctcttccagg ccaaagcttg
      841 cttgccagcg ttttttgccg ctcaacaaat cacctgcgat cgccgtggct tcggtatcct
      901 gctgatcatc tttaaacaac atatccgtgc tggaaaacac cagcatcttg tcctgataag
      961 tcgcgaacat cagggcgtta ttgccgttat agcgcaactg ataaacagga atagtttcac
     1021 tatttatctt gatgctactg atttccgttt tgcttaactg gctgtcgctg gtagcggcaa
     1081 acaacaatgg ctccagcagt ttgcttaaac cactgcgctg gatcagcacc atgtaatgtg
     1141 aaagatggcc ttgtttatcg tgccatagcg cagcctgcgc gggctgatct aagagtgacg
     1201 aaaagagttt atctttcagc gtgagatcgt gttcatagac aatgcgacga atgctgcctt
     1261 caatgcccag
//
LOCUS       DAJVQG010000304         1239 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJVQG010000304 DAJVQG010000000
VERSION     DAJVQG010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.310"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..475)
                     /locus_tag="OQM83_004094"
     CDS             complement(<1..475)
                     /locus_tag="OQM83_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCS1522192.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPFLLFKNLHREFIFSLDGAATEHD
                     TLLPRFTDDISRQSWVSEKVFCNPPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNRLTIRSPFPSAVVVFKKEPRKHEITQMVCC"
     gene            complement(844..>1239)
                     /locus_tag="OQM83_004095"
     CDS             complement(844..>1239)
                     /locus_tag="OQM83_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1522193.1"
                     /translation="SLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISL
                     REGFDLSSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAV
                     QMLISQGKTRLQIQEELGISRATYYRLAK"
BASE COUNT          359 a          278 c          217 g          385 t
ORIGIN      
        1 cacagcaaac catctgtgta atttcatgct tacgtggttc ttttttaaaa acaacgactg
       61 cacttggaaa cggtgaccta atagtaagcc tgttatgccc tgctggtgga aggtatttaa
      121 ctgcacgatg aagaatacgg atttcatgac agtggttatt gctgtaaatg tgcctaagcc
      181 agtagctggt gttggctcta tgcggtatca ggaaagctac taaatccgct tcagacgctt
      241 ttaagaggaa cgacggaata tcgctgtacg gcgggttaca gaagaccttt tcactaaccc
      301 atgattgacg gcttatatca tcggtgaatc gtggtaacag cgtgtcatgc tcagttgcag
      361 caccatctag actgaaaata aattcacggt gtaagttctt aaataacaaa aatggagttc
      421 tccagtaatc agaaccccca tgatgcttcc cgattaaaat atgctcaatt gacataagaa
      481 ataaaaattt ttattgcaat cactcttgac aaggtttact ctcttttaag agatgcttaa
      541 caggttttct aaacggatat cctttctggg atatctatct tttatcttaa aagtttttac
      601 agatacttta aagatatctg tatatattct gtacagtaac ttttcagagt ctgaaaattt
      661 ttataaaaaa tgactagccc tctttgaatc tcatcttccc cgaatcttcg aaaatatctg
      721 tctagttgat tcggtagaat ctacctcatg atgtatacgt gtttagtacc cgtcacacca
      781 gctatgtgac ccccttggca aaaaagccaa atatcagcta catccatgta actattcagt
      841 aacttattta gcaagtctgt aataagttgc tctagagatt cctagctctt cctgaatctg
      901 caacctcgtt ttaccttgtg aaataagcat ctgtacagcc tctgaagaag cttttacagg
      961 tcttcccata tgtacccctt cagctttagc acgttcaata ccagcctttc tacgctctgc
     1021 tatcaagctt ctttcaagct gtgccaacgt tgacataatg cctaacattg cttcacccat
     1081 agcgcttgat aagtcaaatc cttctctaag gctgataact gtcacacctt tagcctgtag
     1141 cgctttaacc gttgataaca catcaattgt gtcacgtcca agacggtcta cagctaccac
     1201 aactacggtg tcaccttctc gtgcataagc taacaaact
//
LOCUS       DAJVQG010000305         1236 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJVQG010000305 DAJVQG010000000
VERSION     DAJVQG010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.311"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..442)
                     /locus_tag="OQM83_004096"
     CDS             complement(<1..442)
                     /locus_tag="OQM83_004096"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCS1522194.1"
                     /translation="MTIGTKEKVIIINELRQCHPLSQLLVIADLPRSTFYYHVKRLNA
                     PDPYQLVKQVILRIYHQHKGRYGYRRIRLACRNESILLNGKTIRKLMKELGISSLIRR
                     KKYRTYRGEQGRTCNNLLKRQFYADRPNQKWVTDVTEFKVDGRKL"
     gene            533..1236
                     /locus_tag="OQM83_004097"
                     /pseudo
     CDS             533..1236
                     /locus_tag="OQM83_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    813..929
                     /locus_tag="OQM83_004097"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          311 a          283 c          317 g          325 t
ORIGIN      
        1 agagcttcct gccatcgacc ttaaactctg tgacatctgt tacccacttt tgatttgggc
       61 ggtcagcata aaattgtctc ttcagtaaat tattacaagt tcgcccctgt tctccacgat
      121 aggtacggta cttcttgcgc ctgataagtg agctgatacc cagttccttc attaattttc
      181 tgatggtttt accattaagt aaaatgctct cgttacggca ggcaagtctg atgcgtcgat
      241 aaccataacg ccctttatgc tgatggtata ttctcaatat tacttgttta acaagttgat
      301 atggatctgg cgcattaagg cgtttcacgt gatagtaaaa tgtactacgt ggcaaatccg
      361 cgataaccag taattgtgat aatggatgac attgccttaa ctcattaata atgattactt
      421 tttcttttgt tccaatcgta atttttcctg agctaaggct ttcagctttt ttagataatg
      481 aaccgccccg ggaatcctgg agactaaact ccctgagaaa gaggtaaaca ggatgactaa
      541 aaatactcgt tttcccccga agtccgtcaa cgggcagtcc gtatggttct ggaaagtcag
      601 ggcgaatatg actcacaatg ggcggtaatt tgttccatta ctccaaagat tggctgtaca
      661 ccggagactc tgcgtgtctg ggttcgccag catgagcggg ataccagggg cggtgatgga
      721 gggctcacca ccgctgaacg tcagcgtctg aaagagctgg agcgtgagaa tcgtgaactg
      781 cgctgcagta acgatatcct tcgccaggct tccgcttatt ttgcgaaggc ggagttcgac
      841 cgcctctgga aaaaataatg ccactgctgg ataagctgcg tgggcagtac ggggtcggac
      901 cggtatgcag tgaactgcat attgccccgt caacgtatta ccactgtcag caacaggaca
      961 tcatcctgat aaacgcagtg cccgtgcgca gcacgatgac tggctgaaga gagagataca
     1021 gcgcgtatac gatgaaaatc atcaggtgta cggtgtgcgt aaagtctggc gccagttgtt
     1081 acgggaagga atcagggtgg ccagatgtac agtggcgcgc ctcatggcgg ttatgggact
     1141 tgtcggtgtt ctccggggta aaaaggtccg cactaccgtc agccggaaaa ccgttgccgc
     1201 aggtgaccgc gtaaaccgtc agttcgtggc agaacg
//
LOCUS       DAJVQG010000306         1227 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJVQG010000306 DAJVQG010000000
VERSION     DAJVQG010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.312"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>1227
                     /locus_tag="OQM83_004098"
     CDS             <1..>1227
                     /locus_tag="OQM83_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCS1522195.1"
                     /translation="SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTE
                     IIDVKQCYPNTALVGVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDG
                     TLKPAYSNNMAWCLWDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVTDGFGGTE
                     PRITCNSYLTTQRKAWDVLSDFCSAMRCMPVWNGQMLTFVQDRPSDKVWTYNRSNVVM
                     PDDGAPFRYSFSALKDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAF
                     GCTSRGQAHRAGLWLIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGR
                     VLAVNSQTRTLTLDREITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDG
                     VAEYSVWGLKLPTLRQR"
BASE COUNT          266 a          326 c          398 g          237 t
ORIGIN      
        1 tcgcagtatc tggcctcggt ggtggtgggt aacctgccgc cgcgcccgtt taatatccgg
       61 atgcgcagga tgacaccgga cagcaccaca gaccagctgc agaacaaaac gctctggtcg
      121 tcgtacaccg aaatcatcga tgtgaaacag tgctacccga acacggcgct ggtcggcgtg
      181 caggtggatt cagagcagtt cggtaaccag caggtgagtc gcaattatca tcttcgcggg
      241 cgcattctgc aggtgccgtc gaactataac ccgcagacgc ggcaatacag cggtatctgg
      301 gacggaacgc ttaagccagc atacagcaac aacatggcct ggtgtctgtg ggacatgctc
      361 actcatccgc gctacggcat ggggaaacgt cttggtgcgg cagatgtgga caaatgggcg
      421 ctgtatgtca tcggccagta ctgcgaccag tcagtgacgg acggatttgg cggcacggag
      481 ccgcgcatca cctgtaactc ttacctgacc acacagcgta aggcgtggga tgttctcagt
      541 gatttctgct cggcgatgcg ctgtatgccg gtatggaacg gacagatgct gacgttcgtg
      601 caggaccgac cgtcggataa ggtgtggacc tataaccgca gtaatgtggt gatgccggac
      661 gatggcgcgc cgttccgcta cagcttcagc gccctgaagg accgccataa tgccgttgag
      721 gtgaactgga ttgacccaga taacggctgg gagacggcga cagagcttgt ggaggatacg
      781 caggccattg cccgttacgg tcgtaatgtc acgaagatgg atgcctttgg ctgtaccagc
      841 cgggggcagg cacaccgagc cgggctgtgg ctgattaaaa cggaactgct ggaaacgcag
      901 accgtggact tcagcgtggg cgcagaaggg cttcgccatg taccgggtga tgtcattgaa
      961 atctgcgatg atgattatgc cggtatcagc accggtggtc gcgtactggc ggtgaacagc
     1021 cagacccgga cgctgacgct cgaccgtgaa atcacgctgc cgtcctccgg taccacgctg
     1081 ataagcctgg ttgacggaag tggcaatccg gtcagcgtgg aggtccagtc cgtcaccgac
     1141 ggcgtgaagg tgaaagtgag ccgggttcct gacggcgttg cagaatacag cgtgtggggg
     1201 ctgaagctgc cgacgctgcg ccagcgt
//
LOCUS       DAJVQG010000307         1226 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJVQG010000307 DAJVQG010000000
VERSION     DAJVQG010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.313"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..695)
                     /locus_tag="OQM83_004099"
     CDS             complement(<1..695)
                     /locus_tag="OQM83_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1522196.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
     gene            complement(1011..>1226)
                     /locus_tag="OQM83_004100"
     CDS             complement(1011..>1226)
                     /locus_tag="OQM83_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HCS1522197.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT          355 a          212 c          259 g          400 t
ORIGIN      
        1 tgggcggttt gttgtaacaa agtttgcatt attctgggag atagttgatt ttctctaaga
       61 ttgacaacgc tatcagaact gaggccaagt atgctttccg gaattgccgt gatccggttc
      121 tgattaagcc agaatattct tataatatga tcatctcttt ctggaaaatc tggtattact
      181 tccaaagcat ttctggctgc atcaagtaat tccaatgaca ttggtaacct cggaagtaca
      241 gacaggtgat tgtcacttac attaatatat tctagtgacg caggtaattc aggaagtgcg
      301 aacaaatggt tatcactcac atttatatat tccagcgaca ctggtaattc aggaagtgca
      361 gataattgat tactgcttgc attcagctct ttcaatgccc ttggtagctc ggggagcatt
      421 gatagttggt tattgcttac attaatttca tcaagattgt caggcaatcg tggtagagat
      481 ctgagaccta aacaagataa gtccaacgat gtttcactgt tctccagaca taattggagc
      541 cgggtaaaag cagtttccct gttttctccg gaaatgctgt ttttagtcca ttcaacccat
      601 tcggagagat aattattgtg aacattgtcg attgatgtag ttctgtaaaa agagacgttt
      661 ccagtggata gggggggatt atttacagga agcataataa cctcgcagaa ggatattctg
      721 ataaaatgtg atactgggaa ataataagct gaaataaaat gtttcaggat tgaaatttat
      781 tgttttcatt ttaaggaaga gatgttgatt taactgtaaa aaaacgatac tattttctta
      841 gatggtaaat gtctcgcctg tgctatattt tgtttttcgg ttagttaaat atcgtacgtt
      901 catatttgaa cgttctgccg gaatgcatta tcaatagagg taaagtcgca accccaaatc
      961 gtaaaggaaa ccgtagcacg tcttaagcaa gaacgtgctg cggttggatg ctattttttc
     1021 cctgaagcgg aaaacattac tacagtacct tgaaccttgg ttttaacatt ctcgaaatgc
     1081 tctgagagta tatgtgttaa gccttcttcg gaatcttttg tgtttgaaaa gatgcctttc
     1141 tgattgtaaa tgcgcatcag tttttgaccg aagctattgt gcacaactcc atcgccaaga
     1201 attgtggctc cgtatagagt tccatc
//
LOCUS       DAJVQG010000308         1212 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJVQG010000308 DAJVQG010000000
VERSION     DAJVQG010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.314"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="OQM83_004101"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="OQM83_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCS1522198.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
BASE COUNT          330 a          299 c          265 g          318 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tg
//
LOCUS       DAJVQG010000309         1188 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJVQG010000309 DAJVQG010000000
VERSION     DAJVQG010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.315"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>1188
                     /locus_tag="OQM83_004102"
     rRNA            <1..>1188
                     /locus_tag="OQM83_004102"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          298 a          267 c          367 g          256 t
ORIGIN      
        1 gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag
       61 gcaaatccgg aaaatcaagg ctgaggcgtg atgacgaggc actacggtgc tgaagcaaca
      121 aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac
      181 cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact
      241 aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tatgtaggtg aagtcccttg
      301 cggatggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat taaaaacaca
      361 gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt gccggaaggt
      421 taattgatgg ggtcagcgca agcgaagctc ttgatcgaag ccccggtaaa cggcggccgt
      481 aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac ctgcacgaat
      541 ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac tcgctgtgaa
      601 gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat agcttgacac
      661 tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg acgccagtct
      721 gcatggagcc ggccttgaaa taccaccctt taatgtttga tgttctaacg tggacccgtg
      781 atccgggttg cggacagtgt ctggtgggta gtttgactgg ggcggtctcc tcctaaagag
      841 taacggagga gcacgaaggt tggctaatcc tggtcggaca tcaggaggtt agtgcaatgg
      901 cataagccag cttgactgcg agcgtgacgg cgcgagcagg tgcgaaagca ggtcatagtg
      961 atccggtggt tctgaatgga agggccatcg ctcaacggat aaaaggtact ccggggataa
     1021 caggctgata ccgcccaaga gttcatatcg acggcggtgt ttggcacctc gatgtcggct
     1081 catcacatcc tggggctgaa gtaggtccca agggtatggc tgttcgccat ttaaagtggt
     1141 acgcgagctg ggtttagaac gtcgtgagac agttcggtcc ctatctgc
//
LOCUS       DAJVQG010000310         1148 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJVQG010000310 DAJVQG010000000
VERSION     DAJVQG010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.316"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            119..376
                     /locus_tag="OQM83_004103"
     CDS             119..376
                     /locus_tag="OQM83_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522199.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            376..750
                     /locus_tag="OQM83_004104"
     CDS             376..750
                     /locus_tag="OQM83_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCS1522200.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            747..>1148
                     /locus_tag="OQM83_004105"
     CDS             747..>1148
                     /locus_tag="OQM83_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS1522201.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNK"
BASE COUNT          325 a          251 c          317 g          255 t
ORIGIN      
        1 gacattttct gcctgtggac caggaaagtg cacgggtgct ttccactccc atgaagcccg
       61 gcaatccgcg cagatacagt aataaacgaa ctgacagcag taagatggag cgtttctttt
      121 gaacaaccag acaatgactt ttacccctga acaattgcgt aaacacgcgc aggaaatgct
      181 gcgtcatgct gaacaactcg aaaaaacggg cataacaaaa gacgctatcc gtaaagatat
      241 ggtacccgcg cttcgggaac tgatgcaggc gaagcatcgc gcacagaaag cagtagatga
      301 gctggtggac tgtgtggcag agctggaaac cagagtcgga aagtttgaaa aactggtgca
      361 ggaggtgctg cgctgatgcg ccatgagttt attttacctt atccgccgac ggtgaatact
      421 tactggcgac gtcgtgacaa cacatatttt gtatcaaaag ccggtgagcg ttatcgccgg
      481 gatgtggcgc ttattgtccg tcagcagcga ctgaaattaa gcctgtccgg aagactggcg
      541 ataaagatta ttgcagagcc accggataag cgccgtcgtg acctggacaa tatcctgaaa
      601 gcaccactgg atgcactgac acatgcggga gtgttaatgg acgatgagca gtttgatgaa
      661 atcaatatcg ttcgtggtca gccagtatct ggtggacgtc tgggggtgaa gatttacccc
      721 ataatgcatg aagagcaggt caaaaaatga aactggaaga tttaccgaaa tactactccc
      781 caaaatcccc ttgcctgacc gatgcatcgg cctcaacgtc aaaagatgcg ctgagtatca
      841 ctgatgtgat ggccgcgcag ggcatgacac agaatcgggc tgagatgggg ttttctgcgt
      901 tcctggggaa aatgggcatc agtatgaatg acagggcgcg ggcaacagaa ttactggcag
      961 attatgcact cagtcggtgc gatcgtgtgg cggcgttgag aaaacttccg gcagaaataa
     1021 aaccggtagt gatgcgcatt atggcttcgt acgcttttga ggattatgcc cgcagcgcag
     1081 cgagtaaaaa gcagtgccct tgttgctatg gggaaaaatt tattgaaagc gtagttttta
     1141 caaacaag
//
LOCUS       DAJVQG010000311         1124 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJVQG010000311 DAJVQG010000000
VERSION     DAJVQG010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.317"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..906)
                     /locus_tag="OQM83_004106"
     CDS             complement(<1..906)
                     /locus_tag="OQM83_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HCS1522202.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
BASE COUNT          329 a          245 c          217 g          333 t
ORIGIN      
        1 aatatcaaag gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg
       61 accatctcca tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct
      121 gtagctgtca tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat
      181 cgccccgcca taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata
      241 acctggagga tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc
      301 atcactgtaa cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata
      361 gacgtcatta ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg
      421 aaacatggcg ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata
      481 gattgcgcca ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag
      541 cgttgagttt tctttagcaa atatggcccc gccgttataa tattctcctg taacggtgtt
      601 attggcaaac agagtcattc cagtttcgtt ttctggtgat aatgtaaatt ccgctccttg
      661 ttgcaaaaat acggccccac cgctggcatt attggtcata tccgaaaaaa ccagccattg
      721 cccatcggcg atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca
      781 actcgctttg atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga
      841 gaaaagagat gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat
      901 ccgcatatta atctgaccat cattttatat aagacggcat atttaaacgc attatacaat
      961 atgggttatc tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt
     1021 tgatgaataa tatcagtgcg tcataattca agttaataac cttaagggat accagttata
     1081 tttaaactaa attatagtaa tgaataattt tcttataata taag
//
LOCUS       DAJVQG010000312         1109 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJVQG010000312 DAJVQG010000000
VERSION     DAJVQG010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.318"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            759..1055
                     /locus_tag="OQM83_004107"
     CDS             759..1055
                     /locus_tag="OQM83_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCS1522203.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT          330 a          213 c          236 g          330 t
ORIGIN      
        1 ttagctgtct cataatccaa aaacaacctt ttgagactac ttgagggtgc attctaccgt
       61 tacgttgaaa gtgtctcaaa aggaagtcct tttaagactg gttatgaagg agctatgagg
      121 ttgattaggg gttggtacaa gcccaactaa ccgaatcaac tatgaagatt ttaggagggt
      181 aactgtaacc atcactttct aactttcacg taggaaattg ttaagtggtt tgatagttac
      241 accatgtgac caagcatcta tatatcgtta tatagccctg tatatagcac tatatgtagt
      301 gtcgagagag gaacgactaa cacgatacag tttaccctga aaagagactt cacagattct
      361 ttatatattt caataagtta gggtaaaaag ggactatacc gagactgtat agaatctgtg
      421 tagaattgca tagatacttg gaaatctact attaaattgc atagggactc acctgatatt
      481 gtcctgtttt taacaggtct cctagaaaac cgaatagact tcattcaagg gtcattgaag
      541 tcataatcat ttcagtagaa aaaccaagtc tcgattttaa atgcaaatag caatcatatt
      601 caataaggca agacgatcag gatcagtgaa aatgtcattt ttggcgtaat tgaccttact
      661 ttatgcatgg tcttaacaaa gacatcatct ttccgttgca gatcttcgcc ctttcaaata
      721 acgtacttta caactttccc gaacaaggag ttgtgcccgt gttgtatgtt atttacgctc
      781 aagacaaagc tgatagcctc gaaaaacgcc tttccgttcg tccggcacat ttagcacgtt
      841 tacagttact gcatgatgaa ggtcgtttgt tgacagctgg tccaatgcca gcggtagaca
      901 gtaacgatcc aggggctgcg ggatttactg gctcaaccgt tattgctgaa tttgaatcgc
      961 tggaagcagc tcaggcctgg gccgatgctg acccgtacgt agcggcaggt gtctatgaac
     1021 acgtttcagt gaaaccattt aagaaagtgt tctgaataga gattcaatat ttaagggcac
     1081 ggtttttgca accgtgttct tgtttggcc
//
LOCUS       DAJVQG010000313         1088 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJVQG010000313 DAJVQG010000000
VERSION     DAJVQG010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.319"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            212..>1088
                     /gene="nadA"
                     /locus_tag="OQM83_004108"
     CDS             212..>1088
                     /gene="nadA"
                     /locus_tag="OQM83_004108"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCS1522204.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
BASE COUNT          294 a          259 c          265 g          270 t
ORIGIN      
        1 caggcatcct caatttcact ttgtaaacct gatgacatcg tcagagctta ctgtgcaagc
       61 aactctacgt cggtggaatt aggcgtaatt cgagtgactt ttccccacca ttcgactatc
      121 ttgtttagca tataaaacaa attacgccga tagtagcgaa tattacgcta atgtcggttt
      181 taacgttaag cctgtaaaac gagatggtaa gatgagcgtg atgtttgatc cagacacggc
      241 gatttatcct ttccccccga agccgacgcc gttaagcatt gatgaaaaag cgtattaccg
      301 cgagaagata aaacgtctgc taaaagaacg taatgcggtg atggttgccc actactatac
      361 cgaccccgaa attcaacaac tggcagaaga aaccggtggc tgtatttctg attctctgga
      421 aatggcgcgc ttcggtgcaa agcatcccgc ttctacgttg ttagtcgctg gggtgagatt
      481 tatgggagaa accgccaaaa ttctcagtcc ggaaaaaaca attctgatgc tgacacttca
      541 ggctgaatgt tcactggatc tcggctgccc tgttgaagaa tttaacgcat tttgcgatgc
      601 ccatcccgat catactgtcg tcgtctacgc caacacttct gctgcggtaa aagcgcgcgc
      661 agattgggtg gtaacttcaa gcattgccgt cgaacttatt gatcatctgg atagttttgg
      721 tgaaaaaatc atctgggcac ccgacaaaca tctggggcgt tacgtgcaaa aacagacggg
      781 cgcagacatt ttatgctggc agggtgcctg tattgtgcat gatgaattta agactcaggc
      841 gttaacccgc ttgcaagaag aatacccgga tgctgccata ctggtgcatc cagaatcacc
      901 acaagctatt gtcgatatgg cggatgcggt cggttccacc agtcaactga tcgctgctgc
      961 gaaaacattg ccacatcagc ggcttatcgt ggcaaccgat cggggcattt tctacaaaat
     1021 gcagcaggcg gtgccagata aagagttact ggaagcacca accgcaggtg agggtgcaac
     1081 ctgccgca
//
LOCUS       DAJVQG010000314         1076 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJVQG010000314 DAJVQG010000000
VERSION     DAJVQG010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.320"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..657)
                     /locus_tag="OQM83_004109"
     CDS             complement(<1..657)
                     /locus_tag="OQM83_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCS1522205.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     L"
     gene            complement(929..>1076)
                     /locus_tag="OQM83_004110"
     CDS             complement(929..>1076)
                     /locus_tag="OQM83_004110"
                     /inference="COORDINATES: protein motif:HMM:NF023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HCS1522206.1"
                     /translation="PARREDEDVKEIRWMIEELRVSYFAQQLGTPYPISDKRILQAME
                     QISG"
BASE COUNT          279 a          260 c          252 g          285 t
ORIGIN      
        1 taaacgcggc agctcgccag tgagtagtga gagataacgc tcaaccggcc ataatccttg
       61 taactgatta ataaagatta cactgtctgc gttatttcct aactgaggaa cgaatccggg
      121 ataacttaac cagcgtggtg catcgggatt gtccccagtc atacggcgga acgtcgccat
      181 cgtgcgccgc tgcatggtgg ggtcctgaac aacgatagcc gtatgaactc gttctacggc
      241 ctgattcaat agcgcgatgc taaagcgtgc gttttcaccg cagtttgttg actggtcttc
      301 aatccagatt ttttcatgcg gaatgtgcca gaactgatga gcgatatccg cctggatggt
      361 cgcttctgct ctgccagtgg tgcggatagt gttgtagtgc ggatgctgtg cgatggcgct
      421 atacaaaaaa gttgtcgagt gaccgatacc accactaatc agtaaaggaa tttgctgatc
      481 gcgagcaatc ttacatgccg cgtcgatagt cggcataacc gcattgcctg caaggatcac
      541 gcaatcggcc tgatacggca cctcaccgga gaaatcatcc tgcgccagcc actgtccgat
      601 aacatttatg gcatctatag ttgccggcga aagcgtcgga aacggggtaa tgttcatggt
      661 tcctccttat cgttccctta agaatactgc gtgagataat agtgagaagc ggagcactct
      721 gaaaggagga agtcgtattt ccttaaccgc cactccgcaa gcctgacata tcttttaagg
      781 aaatgctgta gataaacatt attcatcttg cagcagagaa ttgtattttt ctgaattcct
      841 gaatttttac ccttaccgtt ttagaattaa taaaaaaccc ggcgctaaat gcttaccggg
      901 ttttttcttt atcaggcaaa tagcagggtt aaccgctaat ctgctccatc gcctgcaaaa
      961 tacgcttatc tgaaatcgga taaggcgtac caagttgttg agcgaagtaa ctaacacgca
     1021 actcttctat catccaacgg atctctttca cgtcttcatc ctcacgacgt gcaggc
//
LOCUS       DAJVQG010000315         1070 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJVQG010000315 DAJVQG010000000
VERSION     DAJVQG010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.321"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..478)
                     /locus_tag="OQM83_004111"
     CDS             complement(<1..478)
                     /locus_tag="OQM83_004111"
                     /inference="COORDINATES: protein motif:HMM:NF015679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HCS1522207.1"
                     /translation="MYTRIHWHQYVTPLGLERVISVYGPPAPDRAGAETPLVLTDMTG
                     GVTIYYDPETGDSMTFDSSNRIDRRSQRGAGDPYTGEVVGCETNGSGISAAYGTTKIY
                     TTDTRARWLHGGGSSLRDPYAPRQGWKPTMGCTRAQNEDVDELCKKVTSWMYSHPGE"
     gene            complement(447..1070)
                     /locus_tag="OQM83_004112"
                     /pseudo
     CDS             complement(447..1070)
                     /locus_tag="OQM83_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          214 a          353 c          231 g          272 t
ORIGIN      
        1 tctctccagg atgcgaatac atccaacttg tcactttctt acacaactca tctacatctt
       61 cattctgtgc acgggtacat cccatagttg gtttccagcc ttgtcgagga gcataaggat
      121 cgcgcaacga tgatccccca ccatgtagcc atcttgctct agtatctgta gtatagatct
      181 tcgttgtgcc atatgctgcc gatattcctg atccatttgt ctcacaacca acgacttcac
      241 ctgtatatgg atcaccagca ccacgttgag aacgacgatc aattctattg ctactatcaa
      301 aagtcattga atcacctgtt tcaggatcat aataaatagt cactcctcca gtcatatcag
      361 taagaacaag aggagtttca gcacctgccc ggtctggagc gggaggccca tatacactta
      421 taactcgttc taaccccagt ggtgtaacgt attggtgcca gtggatacgt gtatagattt
      481 attcccccct tcaaccctat cggatcctgg gtgatatacc gcccctgcaa cggatcgtag
      541 taccggtgac ggttgtaata cagtcccgac tcctcatcat actgctgccc cggcaaacgg
      601 tacggctggt acaggtgatg cgggttctcc tcattaagct ggttccccca ctcgtcatac
      661 tccccgcgcc acgccgtttc cccttccggg ctgatgagcg cctgcggcag tccccggtgg
      721 tcacagtggt aaagatgcag cctccgctcc gggatgtatt ccgcctccat ctggctttcc
      781 atctgctcca ccgtcagccc gcactgcgca agccatgcct cactctccgc actcaccgca
      841 cccgcacgca gttcccgctc cagcctcccc agcatcaccg ccagctccgc cggaagcgtc
      901 acccccgtgt cctcctgcaa cacctccgcc aggctccggt gccgcgcctt cgcctgttcg
      961 ccattctctg tttcgattct caggagcggc gtgaagcttc ccggctggta taccgtctgg
     1021 attcttgtct gttgggtctg tacagtggtc agcctgtcac cgtcccaccc
//
LOCUS       DAJVQG010000316         1021 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJVQG010000316 DAJVQG010000000
VERSION     DAJVQG010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.322"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(1..920)
                     /locus_tag="OQM83_004113"
                     /pseudo
     CDS             complement(1..920)
                     /locus_tag="OQM83_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
BASE COUNT          303 a          230 c          180 g          308 t
ORIGIN      
        1 cgttaacttt ctgcgatagc agcagatatg ccagtaaaga aaccccatta gactattttt
       61 ttgataatct tcttcgcttt cgaacaactc gtgcgccttt cgagaagcaa gcattatata
      121 atgccaggcc agttcttctt caattgtccc gttttgaaaa gctgtgcttg atatcgaggt
      181 catccatgat aattccgccg cccatattag cttcgccgag gatttaccgg agcgattatt
      241 agcgcaatca gagatatagt ctgagggaaa aacagcaaat ttattcaaca aggcgataac
      301 ctgctctggg gcttcctcca tgtttgcttt aaaggtattg gctccatggt cgccagaaag
      361 aaaatgctcc attaggcaca ataactttcg ctatcttcga taccccattg attctctaaa
      421 gactcgcgtc ttttactgat gatatcgatc gagtcaaaag gaagcacatg atattggaag
      481 gtatctttac caggttcagg ctttcgcggc cagaactcta gcgtttcaga ccattgctta
      541 tgatagaatc gataaggtgc gatcaattgt agcgcctgta acttctcgat actgagcggc
      601 tcaatacctt tagcctgata ataatgcagt tgttcttttt ttgctttaaa accggcccga
      661 acaataagcc ccatcataat taatagataa agaaaagagc atcccgcggt aatcaggcct
      721 ctttcattca aaccgttgga tgttatcgct gcgaacacaa acattacagc gacaacacat
      781 gttaaataaa acccccactt acaaagcagc atggccttat tttctttaat catccgttca
      841 aaattactat taaatatttc ccagccatta aaagaatact tctcgctccc aggatggttt
      901 tgtaataaaa cttttttcat cacattccct gttattacat actttagagg cgataatttt
      961 atccatgcaa aaaaaatatc caacgaattt tcttgatccg ctggatattt cagaatatga
     1021 c
//
LOCUS       DAJVQG010000317          991 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJVQG010000317 DAJVQG010000000
VERSION     DAJVQG010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.323"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..984
                     /gene="htrE"
                     /locus_tag="OQM83_004114"
     CDS             <1..984
                     /gene="htrE"
                     /locus_tag="OQM83_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HCS1522208.1"
                     /translation="NRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLSFTIPI
                     EKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYTMNKASK
                     DLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLHSGGLTFSNDSFSDSD
                     TLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIALDINDLENDVELKSTS
                     AVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIYDEQGNVIGNVGQGGQA
                     FVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSIILNGIRCQIQ"
BASE COUNT          289 a          197 c          242 g          263 t
ORIGIN      
        1 aatcgtagca attactctat tggctacagt aacagtgcat cctggggcag ctacagtgtc
       61 agtgcccagc gttcatggaa tgaagacggc gacactgacg atagcgttta tctcagtttc
      121 accattccta ttgaaaaatt acttggcact gaacaacgga cttcaggttt ccagagtatt
      181 gatactcaaa tgagcagtga ctttaagggt aataaccaac tcaacgtcag tagcagtggc
      241 tatagtgata acgctcgcgt cagttatagc gtaaatactg gctatacgat gaataaagcc
      301 agcaaagatt tgagttatgt tgggggttat gccagctatg aatcaccgtg gggaaccctg
      361 gcaggttcag tttctgcaaa tagcgataac agtcgtcaag tgtctctcag caccgacggt
      421 ggttttgtat tgcatagcgg cggcctgacc ttcagtaatg acagttttag cgactccgat
      481 acactggcgg tagttcaggc tccaggtgct caaggagcgc gaataaatta tggcaacagt
      541 actatcgatc gatggggtta tggtgtcacc agcgctcttt ctccttatca tgaaaaccgt
      601 atcgcgctgg atatcaacga tcttgagaac gatgttgaat taaaaagtac cagtgcagta
      661 gctgtaccgc gtcagggttc agtcgtcttt gctgattttg aaaccgtgca agggcaatca
      721 gccattatga acatcacacg aagtgatggt aaaaatattc catttgctgc agatatttat
      781 gatgagcaag gcaatgtcat tggtaatgtt ggacagggtg gacaagcatt tgttcgtggc
      841 attgagcagc agggaaatat cagcattaaa tggctcgaag aaagtaaacc cgtaagttgt
      901 cttgcgcatt atcaacaaag ttcagaagca gaaaaaatag cgcaatctat tattctgaat
      961 ggaattcggt gtcagattca gtaactacaa g
//
LOCUS       DAJVQG010000318          978 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJVQG010000318 DAJVQG010000000
VERSION     DAJVQG010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.324"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..753
                     /gene="mppA"
                     /locus_tag="OQM83_004115"
     CDS             <1..753
                     /gene="mppA"
                     /locus_tag="OQM83_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCS1522209.1"
                     /translation="PQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGE
                     KPAWHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSE
                     NHQKIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFL
                     TLLTSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQY
                     TNGRLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(804..>978)
                     /locus_tag="OQM83_004116"
     CDS             complement(804..>978)
                     /locus_tag="OQM83_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HCS1522210.1"
                     /translation="NIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQT
                     LYMDNITPPEQGR"
BASE COUNT          290 a          221 c          233 g          234 t
ORIGIN      
        1 ccgcagctcg ggacctatta ttatgcgttt aacacgcaaa aagggccgac ggcagatcag
       61 cgcgttcgtc tggcattaag tatgacgata gatcgccgcc tgatgaccga aaaagtatta
      121 gggacgggcg aaaagccagc gtggcatttt acaccagatg ttaccgccgg atttacgccg
      181 gaaccttcgc cgtttgaaca aatgagtcag gaagaactga atgcgcaggc aaaaactttg
      241 ttgagcgcag ctggttatgg tccgcaaaaa ccgctgaagc tgacgctttt gtataacact
      301 tcagaaaacc atcaaaaaat tgcgattgct gtagcatcga tgtggaaaaa gaaccttggc
      361 gtagatgtta aattgcaaaa tcaggaatgg aaaacctata tcgatagccg taacaccggc
      421 aattttgatg ttatccgcgc ctcatgggtg ggggattata atgaaccctc cactttcctg
      481 acattattaa cgtcaacgca ttcaggaaat atttcacgct ttaacaatcc ggcatacgac
      541 aaagttctgg cccaggcatc gacggaaaat accgttaaag cgcgtaatgc cgattacaac
      601 gcggcagaaa aaatcctcat ggagcaagca ccgattgcac caatttatca atataccaat
      661 ggacgattaa tcaagccgtg gctgaaaggt tatcccatta ataacccgga agatgtggcg
      721 tacagccgga ctatgtatat tgtgaagcat tgatgtgatg ggaactggcg ttacccttgt
      781 ggataacgcc agtgatgtct gatttagcgc ccctgttccg gcggtgtaat gttatccata
      841 tacagcgtct ggctggggaa ggcaaagtcc gcgccgtgtg actgcacaat atcgataatc
      901 ttcaaataaa cgtcttgctg tgcagcaagc cattcagccc ataccgtggt tttggtaaag
      961 caataaacca taatattc
//
LOCUS       DAJVQG010000319          962 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJVQG010000319 DAJVQG010000000
VERSION     DAJVQG010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.325"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            12..>962
                     /locus_tag="OQM83_004117"
     CDS             12..>962
                     /locus_tag="OQM83_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1522211.1"
                     /translation="MVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDIRQFNRA
                     NLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIKVDALKS
                     FNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRWDEGINA
                     LTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGRAEYNSI
                     STWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFAPVVRGI
                     AKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLD"
BASE COUNT          245 a          227 c          230 g          260 t
ORIGIN      
        1 cgaccagaat agtggttggc ctcacggcag ggacgtgtct gattttctcg caaaacctga
       61 tggccgaggt cagtgtattc aatccggcgc ttctggaaat cgaccatcaa tccggagtcg
      121 atattcgcca gtttaatcgg gcaaacctga tgcccccagg tgtttatagc gttgatattt
      181 ttatcaacgg taaaatgttt gaacgtcagg atgtgacatt tgttcaggat aatccagatg
      241 ctgatctgca cgcttgcttt attgccatta aaaaaacact gtcctccttt ggcataaaag
      301 ttgatgcgct caaatcgttc aatgatgtgg atgagacggt ttgcctcgat cctgctccac
      361 gtattgaagg ctcatcctgg cagtttgaca gtgataaatt gcagctgaat atatccattc
      421 cccaaatcta catggacgcg atggcttatg attacatcag ccccacgcgt tgggatgagg
      481 ggattaacgc gctcaccatc aactacgatt tttctggttc acatacacta cgttcagatt
      541 atggttcaca agagacagat accagttatc tcaatctgcg caatggactg aatattggac
      601 cgtggcggct acgtaattac agtactttaa acaccagcga tggccgtgcg gaatacaact
      661 ccattagtac ctggatacag cgcgatattg ccgcgttaag aagccagatt atgattggtg
      721 atacgtggac ggcgagcgat attttcgaca gtacgcaaat tcgcggcgcg cgtttgtata
      781 ctgataacga tatgctaccc gccagccaga atggctttgc tcctgtggtt cgtgggattg
      841 caaagtccaa cgccaccgtc atcattcggc agaatggcta cgtgatttat cagtctgccg
      901 ttccacaagg tgcttttgag atcaccgatc tcaacaccgc cagtacaggt ggcgatttgg
      961 ac
//
LOCUS       DAJVQG010000320          955 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJVQG010000320 DAJVQG010000000
VERSION     DAJVQG010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.326"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            1..607
                     /locus_tag="OQM83_004118"
                     /pseudo
     CDS             1..607
                     /locus_tag="OQM83_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          356 a          173 c          158 g          268 t
ORIGIN      
        1 caggttaatt ttgttagttc aacacaaaac gccacaatct cctgggatta tttactaaaa
       61 ataacgcctg ttttaataag cattagcgat atgtattctg aagaaaaaat caagtttgtc
      121 gaaagttgtt taaatgagct tggagacatt accgaagaac aattaaaaat tatgagattt
      181 gcaattataa aatctatacc aagggcaact cttacagata aattagaaaa tgaattaaca
      241 aaagaaatat ataaaagctc atcgaaaatc aacaattgct tgaacagaat taaattacca
      301 gagatgaaag aattctcatc agaaaaaaat atatgattac atcgatataa tcattgaaga
      361 ttatgaaaat atcaaagaaa atgcttatct ggtcgatcca aaaattaatt acactataga
      421 tttaaacata gaagactcta gctcagagga attccttttt gataatagcc tcgagaaaga
      481 cgaaaattct tcggacaatt gctttgaggt cgaaaaatat aacgcatatg aagcatataa
      541 ctcagagaat ctatatttta ccagagagga atatacgtat gattacgacc ttttaaatgc
      601 aatatagaca taataacttc ttacaaaatc tataattaat accgtagata ttcattcatc
      661 ttaccctgtg caccaaaagc aaaaactcac tgacgcgggt ttgaaactga tggcaaccgc
      721 aaaaatgcct gatgcgctac gcttatcagg cctacgccat ctctacaata tattgaattt
      781 gcgtgctttt gtaggcagga taaggcgttc acgcagcatc cggcatgaat aaagcgcact
      841 caacaatctg aaacccgccg gaacggttta ttacgtacat caggtgaaac tgaccgataa
      901 gccgctttct tttgggtata gtgtcgtgga cagtcattca tctttctgcc cctcc
//
LOCUS       DAJVQG010000321          877 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJVQG010000321 DAJVQG010000000
VERSION     DAJVQG010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.327"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            1..705
                     /locus_tag="OQM83_004119"
                     /pseudo
     CDS             1..705
                     /locus_tag="OQM83_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            712..>877
                     /locus_tag="OQM83_004120"
     CDS             712..>877
                     /locus_tag="OQM83_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCS1522212.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRV"
BASE COUNT          254 a          201 c          256 g          166 t
ORIGIN      
        1 ttattcgctt ctgggcatgg gcggatcaac aaatgataga cggtaatgca gattgtaacg
       61 ctcgcggcgt tacaaaaagt gcaatagatc gcatcacttt tatgtctggt tttgctgatg
      121 cgttaattca ggttggatgg ctggtcgaaa atgacggagg gctttctcta cctaactttg
      181 aacgtcataa cggaaaaagc tctaaaaaaa acgggcggtt acaaacgagc gagtaacaaa
      241 aatacgcgaa ctgaaacgaa aaggtaacgc tgccagcgtt acacaaacgg atcaaaaagc
      301 gttaccagag gaagaggaag aggaagagga agaggaagag gaagaggaag aggaagatct
      361 aaatactgat ctccccctcg ccaaaaacga gcgtctaaaa aattcgagcc ggaggctatt
      421 gagctgcccg attggttgcc ggaaacactc tggcatgagt gggtccagtt caggcaggca
      481 ttgcgaaaac cgattcgaac ggagctgggc gctaacgggg cgatacggga actggaaaaa
      541 ttccgtcagc agggttttac acctgagcag gtgattcgac acagcatcgc caatgaatac
      601 cagggcctgt tcgcgccgaa aggtgttcgg cctgagacgt tgctccgaca ggttaacacc
      661 gtctcgttgc cggacagtgc gatcccgcca ggcttcaggg ggtaacggac catgaaaaat
      721 attgcgacag gcggcgttct ggaacgcatc cgccgactga ccccgccaca tgtaaccgcc
      781 ccattcagaa cggtagcgga gtggcgcgag tggcaacttg ctgaaggcca gaaacgtagc
      841 gaggagatca accgcctgaa tcgccagttg cgggtgg
//
LOCUS       DAJVQG010000322          862 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJVQG010000322 DAJVQG010000000
VERSION     DAJVQG010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.328"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..501
                     /locus_tag="OQM83_004121"
     CDS             <1..501
                     /locus_tag="OQM83_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCS1522213.1"
                     /translation="GYLQTHTDMPGSDDLKGINVKYRYEFTDTLGLITSFSYANAEDE
                     QKTHYSDTRWHEDSVRNRWFSVMAGPSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVT
                     DNKGKTHDVLTGSDGGRHSNTSLAWGAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIV
                     GVGYKF"
     gene            565..>862
                     /locus_tag="OQM83_004122"
     CDS             565..>862
                     /locus_tag="OQM83_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCS1522214.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLR"
BASE COUNT          189 a          194 c          275 g          204 t
ORIGIN      
        1 gggtatcttc agacccatac tgatatgcca ggcagcgatg acctgaaggg cattaacgtg
       61 aaataccgtt atgagtttac ggacacgctg gggctgatta cgtcattcag ttatgccaac
      121 gctgaagatg agcaaaaaac gcattacagc gatacccgct ggcatgagga ttccgtgcgt
      181 aaccgctggt tcagcgtgat ggcggggccg tctgtgcgcg tgaatgaatg gttcagcgcg
      241 tatgcgatgg cgggcgtggc ttacagccgt gtgtcgacct tctccgggga ttatctccgc
      301 gtaactgaca acaaggggaa aacgcacgat gtgctgaccg gaagtgatgg cggtcgccac
      361 agcaacacgt ctctggcgtg gggggctggc gtgcagttta acccgaccga atccgtggcc
      421 attgaccttg cttatgaagg ttccggcagt ggtgactggc gcactgacgg tttcatcgtg
      481 ggtgtcggtt ataagttctg attagccagg taacacagtg ttatgactgc ccgctggttc
      541 aggcgggctt tttgtggggt gaatatggca gtaaagattt caggtgtact gaaagatggg
      601 gcgggtaaac ctgtcgtaaa ttgtgcgatt gaactgcggg ccagaagaac cagtccgacc
      661 gttgtggcac acgttgttgc cacttgcgtg acggacaata acggtgctta tgtgattgag
      721 gctgagccgg ggtattacga ggttgcgctt cactgtaacg gctggcagcc aacccgtgtc
      781 ggggatattg atgtggcacc gactgatgca ccggggacac tgaacgcgtt tctgaatgca
      841 ccaaaggatg gtgatttacg tc
//
LOCUS       DAJVQG010000323          842 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJVQG010000323 DAJVQG010000000
VERSION     DAJVQG010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.329"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(23..>842)
                     /locus_tag="OQM83_004123"
     CDS             complement(23..>842)
                     /locus_tag="OQM83_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1522215.1"
                     /translation="LREEIEGFYPQIVTDSKFYAVQQLLEETGKGKTSSGEHWLYVNI
                     LKGLIRCKCGLVMTPTGIRKPVYQGTYRCNGNKESRCSYGTVSRKLLDTQLCSRLFSK
                     LSQLHDEATDTAKLDELQRRLNTVDGELEKLTETLIQLPNITQIQEALRVKQEEKDEL
                     IVQLSREKARVKSVSSLDLSGLDMESVEGRTEAQIIIKRLVKEIVVSGNEKLVDIYLH
                     NGNMIRGFPLDGKDDHTLTLEEATDEMQSLDDMLIFGEPVTRIYPAGDMEEVDA"
BASE COUNT          231 a          208 c          140 g          263 t
ORIGIN      
        1 cagtgaaccc taacaaaaaa tactaagcgt ccacctcttc catatcacca gcaggataaa
       61 tccttgttac tggttcacca aaaataagca tatcatctaa tgactgcatc tcatcggttg
      121 cttcttcaag cgttaaggtg tggtcatctt taccatcaag aggaaaacca cgaatcatat
      181 ttccattgtg aagatatata tccaccagct tttcattacc agaaacaaca atctctttca
      241 ccagacgctt aataataatc tgggcttcag ttctcccctc tacagactcc atatcaagac
      301 cgcttaaatc caagcttgat acagatttta ccctcgcctt ttctcgtgat aactgaacta
      361 tcagttcatc tttttcttcc tgtttaaccc gtagtgcttc ttgaatctga gtgatgttag
      421 gaagctgtat cagcgtctca gtgagttttt ccagttcacc atcaacggtg tttaatcttc
      481 tttgtagttc atctaattta gctgtatcgg tagcttcatc gtgtagctgt gacagtttgc
      541 taaacagacg actacaaagc tgtgtatcaa gtaactttct ggacactgta ccgtagctac
      601 aacgggactc cttattacca ttgcaacggt aagtaccttg ataaacgggt ttacgtatcc
      661 ctgtaggagt cattacaagc ccacacttac aacggataag ccccttcagt atgttcacat
      721 atagccagtg ttcaccgctg gatgttttcc ccttccctgt ttcctctaac agttgctgta
      781 cagcgtaaaa cttagagtct gtgacaatct gaggatagaa cccttcaatt tcttcacgta
      841 ac
//
LOCUS       DAJVQG010000324          838 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJVQG010000324 DAJVQG010000000
VERSION     DAJVQG010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.330"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(78..743)
                     /locus_tag="OQM83_004124"
     CDS             complement(78..743)
                     /locus_tag="OQM83_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCS1522216.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          215 a          215 c          190 g          218 t
ORIGIN      
        1 gcagatagcc tcaagcaccc gcagtcattc tacttacctc gccaatatga aatcaatcag
       61 aaaggtgccc cataaaatca ctccttctct tctttaccgt agtggagttg accaattttg
      121 ataagagggc gtccctgaga tttgcggtgt agattggtat cgcgcagaga atacacacag
      181 ccacaatatt cctgctgata gaatttttcg cgcttgctga tttcaatcat acgggacgag
      241 ccgccctgct tgcgccagtt ataatcccag tacaccatgc ccggataatg cgcgacggct
      301 cgccgcccac agtcgttaac ctgctgcata tttttccagc gtgaaatgcc cagtgaactg
      361 ctgatcacac tgaaaccatt ttcagcggcg tacagcgctg tccgctcaaa acgcatgtca
      421 aaacacatgg tgcaacggat ccctcgttcg ggctcccatt ccattccttt ggctcgttca
      481 aaccagttgt ctgtgtcgta atcagcatca ataaacggca cgccgtgttg ttcagcaaag
      541 cgaatattct catccttacg aattaaatac tctttctgag gatgaatgtt cgggttgtag
      601 aaaaagatgg tgtagtcgat tcccgaggcc tgaagcgcct ccatcacctc accggaacat
      661 ggagcacagc aagagtgcag tagtagtttg tttgccccgt ttgggagctc caatttgggc
      721 cgtttgaaat cagcaactgt cataaatatg tttattgggg tcataaaaat atcaaagagt
      781 gtagcattag aacggggcta tcggaaacag atgtgtgact gctccccgcc ctttcggg
//
LOCUS       DAJVQG010000325          827 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJVQG010000325 DAJVQG010000000
VERSION     DAJVQG010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.331"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            138..404
                     /gene="hipB"
                     /locus_tag="OQM83_004125"
     CDS             138..404
                     /gene="hipB"
                     /locus_tag="OQM83_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HCS1522217.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            404..>827
                     /gene="hipA"
                     /locus_tag="OQM83_004126"
     CDS             404..>827
                     /gene="hipA"
                     /locus_tag="OQM83_004126"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HCS1522218.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LIPEDETVTRPIMAWEKLTEARLEEVLTAYKADIPLGMI"
BASE COUNT          244 a          189 c          196 g          198 t
ORIGIN      
        1 tttatccgcg atcgcggata tcgcagcgtt tatcccgtac agcggataag atgtgtttcc
       61 agattgactt tatcctcact aaaggataaa actgataata tccccttaag cggataaatt
      121 tgctgtggac gtatgacatg atgagctttc agaagatcta tagcccaacg caattggcga
      181 atgcaatgaa actggttcgc cagcaaaatg gctggacgca gagcgagctg gcgaaaaaaa
      241 ttggcattaa gcaggcgacg atttccaatt tcgaaaacaa ccctgacaat accacgctga
      301 cgaccttttt taagatttta cagtcgcttg aactctcaat gacgctatgc gacgcgaaaa
      361 atgcctcgcc agaagcaaca gaacagcaag atctggagtg gtaatgccta aacttgtcac
      421 ttggatgaac aaccagcggg taggcgagtt aacgaagtta gccaacggcg cgcacacctt
      481 taagtatgca ccggagtggt tagccagccg ttatgccaga ccgttgtcac tttcgctgcc
      541 attgcagagg gggaatatca cctctgatgc cgtatttaac ttcttcgata acctgttacc
      601 cgatagcccg attgtacgtg accggatcgt taaacgttat catgccaaat ccagacaacc
      661 gtttgatttg ttgtcagaaa tagggcgaga cagcgttggt gccgtgacgt taatacccga
      721 agacgaaacc gtaacgcgtc cgataatggc atgggaaaag cttactgaag ccagacttga
      781 agaagtatta acggcttata aagcagatat cccgctaggc atgatta
//
LOCUS       DAJVQG010000326          823 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJVQG010000326 DAJVQG010000000
VERSION     DAJVQG010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.332"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..669
                     /locus_tag="OQM83_004127"
     CDS             <1..669
                     /locus_tag="OQM83_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HCS1522219.1"
                     /translation="LVHGVHYVGRADWGPAHRRWLSKYSFESPWRQLAFDEHRRTIED
                     RQAQCERLESALKEAVTEWRLYPVVEALQAMRGIQFITAVGLISELGDLTRFEHPRQL
                     MSWFGITPSEYSSGGSRHQGSITKAGNSYARKLLVEAAWSYRHPARISPAIQKRQENL
                     PRPVIDRAWDAQLRLCKRYRKLQAKGKNVNITIVAVARELAGFIWDMGRIAMSVAQQP
                     QCHK"
BASE COUNT          231 a          193 c          223 g          176 t
ORIGIN      
        1 ctggttcatg gggtccatta tgtcgggcga gcagattggg gccctgcaca ccgacgctgg
       61 ctcagcaaat actcattcga aagtccctgg cgacaattag cttttgacga acaccgtcgt
      121 acaatagaag atcggcaggc acaatgtgaa cggctggaat ccgccctgaa ggaggccgtt
      181 accgaatgga ggctgtatcc ggtagtagag gcgctgcaag ccatgcgagg gattcagttt
      241 attactgcgg ttggattaat ttctgaactg ggtgatctga cccgttttga acatccacga
      301 caactgatgt catggtttgg tatcacgcca tcagaatatt catcgggtgg aagcagacat
      361 cagggcagca ttaccaaagc cggaaacagc tatgccagaa agctattggt cgaggcagcg
      421 tggagttacc gtcatccggc acgcatcagt ccggcaatac aaaaaaggca ggaaaattta
      481 ccccgccccg tcattgacag agcatgggat gctcaactca ggctttgtaa gaggtatcga
      541 aaacttcagg ccaaaggaaa gaatgtcaat attacaattg ttgctgttgc acgtgagctg
      601 gcgggtttta tctgggatat gggccgaata gcaatgtctg tcgcacaaca accacaatgt
      661 cacaaataaa aaatgagaat aaccagttcc ctgaaggcga cgtagcccgg tacaagagta
      721 acccacgtta gcgcttggcg acggcggaac aaagctgatt cgcgacgtaa gaaagtggca
      781 ggctcatatg acggacctct gtaatgcggt atccaacccg cga
//
LOCUS       DAJVQG010000327          819 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJVQG010000327 DAJVQG010000000
VERSION     DAJVQG010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.333"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            54..788
                     /locus_tag="OQM83_004128"
                     /pseudo
     CDS             54..788
                     /locus_tag="OQM83_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
BASE COUNT          202 a          218 c          179 g          220 t
ORIGIN      
        1 catcaccgtc ttatctcact gttcaccctg taacgttcag agagcgtctt cagatgtcta
       61 tttataaaat tcctcttccg ctcaatattc tggaagctgc acgagagcgt attacctgga
      121 cactcaacac cctaccccgc gtttgtgtct cattttctgg cggcaaaaac tctggtttaa
      181 tgctgcatct gacagccgaa cttgcccgac aaatgggcaa aaaaatctgc gttttgttta
      241 tcgactggga ggcgcaattc tcttgcacca ttaactatgt tcagtccctg cgcgagttgt
      301 acaccgatgt catcgaagag ttttactggg ttgcgctccc gcttacgacg caaaattccc
      361 tttcacaata ccaacccgaa tggcagtgct gggaacctga tgtcgaatgg gtgcgtcagc
      421 ccccgcaaga tgcgataacc gatcctgact ttttctgctt ttaccagcca ggcatgacct
      481 tcgaacaatt tgtacgtgag tttgccgaat ggttttcaca aaaacgtccg gtggcgatga
      541 tgatcggcat ccgtgcggat gagtcctaca accgttttgt cgccatcgcc agtttaaata
      601 aacaacgttt tgccgacgat aaaccatgga ctacggccgc accaggcggt catagctggt
      661 acatttaccc catttacgac tggaaagtgg ctgatatctg gacctggtat gcaaatcatc
      721 aaaatctctg caacccactg tataacctaa tgtatcaggc aggcgttcct ctgcgtcata
      781 tgcgaatttg agaacctttt ggcccggagc aacgacaag
//
LOCUS       DAJVQG010000328          815 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJVQG010000328 DAJVQG010000000
VERSION     DAJVQG010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.334"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>815
                     /locus_tag="OQM83_004129"
     CDS             <1..>815
                     /locus_tag="OQM83_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCS1522220.1"
                     /translation="FNRELAKLNQSLRETLSKFNKTTSKNNHSENASNSLDVLATSAI
                     KAGTAIYSFQTALEAYRRIMEVGLKKEASQRAAQFVLGDEGAKRATEFVKNLADSSGV
                     DQIETLSSFAKFSAGAGDMNADQKESLFSNVIGTSRLMGLSTDEINGILKAFEQMASK
                     GKIQAEELRGQLGDRMAGAFQLFARSLGMTTEELDKAMKDGKVLSKDVFPKVSAEMGR
                     MIDKAGGWEKIINSTQTQLGRLSNSWNNNLALMFDGSQEGLTDFTRSLTNLLNS"
BASE COUNT          264 a          129 c          192 g          230 t
ORIGIN      
        1 tttaatcgtg agttagcaaa gttaaatcag tctcttcgtg aaacactttc taagtttaac
       61 aagaccacca gcaaaaataa tcacagtgaa aatgcttcaa atagtctgga tgtattagct
      121 acgagtgcaa tcaaagcagg tacagccatt tatagttttc agacagcctt agaagcttac
      181 aggcggatta tggaggttgg tctgaaaaaa gaagcatcac aacgagcggc acaattcgtt
      241 ttaggtgatg aaggtgctaa acgagctaca gaatttgtta agaatcttgc tgatagtagt
      301 ggtgtagacc agattgaaac attaagcagt tttgctaagt tttccgctgg tgctggtgat
      361 atgaatgcag accagaaaga atccttattt tctaatgtga ttggtacatc cagattaatg
      421 gggctaagca ccgatgaaat taacgggatt ttaaaagctt tcgagcagat ggcatctaaa
      481 ggaaagattc aggctgaaga gctacgcggt caattaggtg acagaatggc aggtgctttt
      541 cagttatttg ccagaagtct tgggatgact acggaagaat tagacaaggc catgaaagac
      601 ggtaaggttc tttctaaaga tgtttttcct aaagtatccg ctgaaatggg aaggatgatt
      661 gataaagctg gtggatggga aaagattatt aactctactc agacgcagtt aggccgctta
      721 agtaattcat ggaataataa tctggcctta atgttcgatg gttcacagga aggtttaaca
      781 gactttacaa ggtcactgac taatttactt aattc
//
LOCUS       DAJVQG010000329          811 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJVQG010000329 DAJVQG010000000
VERSION     DAJVQG010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.335"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            1..811
                     /locus_tag="OQM83_004130"
                     /pseudo
     CDS             1..811
                     /locus_tag="OQM83_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          224 a          199 c          217 g          171 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      421 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      481 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      541 gcgccaaatc tgctgaacca gacgttcgct cctacagcac caaatcaggt ctgggtggcg
      601 gacctgacgt atgttgccac acaggaggga tggttgtacc tcgctggcat caaagatgtt
      661 tatacgtgcg aaattgtcgg ctacgccatg ggagagcgca tgacaaaaga gctgacaggt
      721 aaagccctgt ttatggcgct caggagccag cgcccacctg ccgggctaat ccaccactct
      781 gatcgaggtt cacagtactg cgcatacgat t
//
LOCUS       DAJVQG010000330          799 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJVQG010000330 DAJVQG010000000
VERSION     DAJVQG010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.336"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            1..799
                     /locus_tag="OQM83_004131"
                     /pseudo
     CDS             1..799
                     /locus_tag="OQM83_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          313 a          146 c          116 g          224 t
ORIGIN      
        1 tggctcaact tgtaagtctt attactaaca ttgaaacgat aagttcgaca caacttacgc
       61 aacagacaca atcaatactt aatcagatta ataacattag atatgaaaaa aacaaaagtg
      121 ctgaatgtcg aatcattgtt gtcgctaatc ctaaacccga caaggcaatc ataactaaaa
      181 ttagcgtaga agagggaata cccataacat tcagtgtcca aaccatgttt tcagatacta
      241 attttattgc tgaacaacga gctgacttac caactaatat aaaagatatt caaagcctgt
      301 atcaaaaaat gacgaagtta tatattgagc acagtgaaaa taaaaacagg atgaaagtct
      361 ttgccggagc caactttatt gatttcaata tgacaggaca gaacctgtcc gggttcgttc
      421 taaccttgtc aaggttttat ttcgaagatc tacttaatat aaatttcact gacgcaaatt
      481 taggtgacac tattttttac ataaagaaca ccctaccccc aaattatata aagatggaca
      541 atatcttgac aaacaaatcg aaggtttatt ttcaacatta ttaaccatta atgacaactt
      601 tttacgagca aaagcggaaa ttgcttcgac aataattaaa tttttagagg ccaggataac
      661 caatctttca tacaatgaca tattaaaata tcagcaagaa ttccaaaaac aatgttataa
      721 gcaagttaaa gcctttacga cactctctcg atacaataaa attcaaacat gggcagaaat
      781 gtctgaatat cagttcgaa
//
LOCUS       DAJVQG010000331          794 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJVQG010000331 DAJVQG010000000
VERSION     DAJVQG010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.337"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>794
                     /locus_tag="OQM83_004132"
     CDS             <1..>794
                     /locus_tag="OQM83_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HCS1522221.1"
                     /translation="HLKITFRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDG
                     IEQSGLHIWSFGQLPESYEQKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNG
                     LRRLLLLNIPSPIKYLHEKLPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGP
                     VWTEEGFAALHEKVRAELNDTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDI
                     KAQMGGLVYRGFVTGNGFKRLGDTLRYLQAIEKRLEKLAVDPHRDR"
BASE COUNT          213 a          171 c          244 g          166 t
ORIGIN      
        1 tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac aagaagctaa aagaagggcg
       61 ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg caggaaacgc tgtctgcggt
      121 ggcggatgac ggtatcgagc agagcggact gcatatctgg agttttggtc agctgccgga
      181 aagctacgaa cagaagcgtg gcaactacaa agtgaaggcg tggccagcgc tagtggatga
      241 acgcgacagt gtggcgatca aactgtttga taatccgctg gagcaaaaac aggtaatgtg
      301 gaacggtctt cgccgtctac tgctgctgaa tattccctcg ccgatcaagt atttacatga
      361 gaagttacca aacaaagcca agctggggct gtactttaac ccgtatggca aagtgctgga
      421 gctgatcgac gactgtatct cctgcggtgt ggataaattg atcgacgcca atggtggccc
      481 ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa gtgcgtgccg aactgaacga
      541 cacggtggtg gatattgcga agcaggtcga gcaaatcctt acggcagtgt tcaatatcaa
      601 caaacgtctg aaggggcggg tggatatgac gatggcgctg gggctttctg acattaaagc
      661 gcagatgggc gggctggtat atcgcggttt tgtcactggt aacggcttca aacggctggg
      721 cgacacgctg cgatatttgc aggcgattga aaaacggctg gaaaaactgg cggttgatcc
      781 acatcgcgac cgtg
//
LOCUS       DAJVQG010000332          790 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJVQG010000332 DAJVQG010000000
VERSION     DAJVQG010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.338"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>790
                     /locus_tag="OQM83_004133"
     CDS             <1..>790
                     /locus_tag="OQM83_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HCS1522222.1"
                     /translation="ECQIRNSNFDNCYLYASHFTRAEFLSAKEISFIKSNLTAVMFDH
                     VRMSTGNFKDCITEQLELTIDYSDIFGNEDLDGYINNIIKMIDTLPDNAMILKSVLAV
                     KLVMQLKILNIVNKNFIENMKKTFSHCPYIKDPIIRSYIHSGEDDKFDNFMRQHRFSK
                     VNFDTQQMIHFINRFNMNKGLIDKNNNFFIQLIDQALRSTDDMIKANAWYLYKEWIRS
                     DDVSPIFIETEEKLRTFNTNKLTRNDNIFILFSSVDDGPVMVVSS"
BASE COUNT          281 a          115 c          134 g          260 t
ORIGIN      
        1 cgaatgtcag attagaaatt caaacttcga taattgttat ctttacgctt cgcacttcac
       61 cagagcagag tttctgtctg ccaaagaaat atcatttatt aaatcgaatt tgacagctgt
      121 tatgtttgat catgtgcgaa tgtcgacagg gaattttaaa gattgcatta cagaacaatt
      181 ggaattaact attgattatt cagatatatt tgggaatgaa gatctcgatg gttatatcaa
      241 taacattata aaaatgattg atacattgcc agataatgca atgatattga aatccgttct
      301 ggcagtaaaa ctggtgatgc aattaaaaat tctcaatatt gttaataaaa actttattga
      361 gaatatgaaa aaaacattta gccattgtcc ttatataaaa gatccaatta tacgtagtta
      421 tatacattct ggggaagatg acaagttcga taattttatg cgtcaacatc gattcagtaa
      481 ggtgaatttc gatacccaac agatgatcca ttttattaac agatttaata tgaataaagg
      541 gctgattgat aaaaataaca atttttttat ccaacttatc gatcaggcct tacgatcaac
      601 ggatgatatg atcaaagcaa atgcctggta tctttataaa gagtggattc gtagtgatga
      661 tgtttcacct atatttatag aaactgaaga aaagttaaga acctttaaca ctaataaatt
      721 aacacgaaac gataatatat ttatcctgtt ctcctctgtc gatgatgggc ctgttatggt
      781 ggtaagctcc
//
LOCUS       DAJVQG010000333          789 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJVQG010000333 DAJVQG010000000
VERSION     DAJVQG010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.339"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..84
                     /locus_tag="OQM83_004134"
     CDS             <1..84
                     /locus_tag="OQM83_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCS1522223.1"
                     /translation="LWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            84..374
                     /locus_tag="OQM83_004135"
     CDS             84..374
                     /locus_tag="OQM83_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCS1522224.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            371..>789
                     /locus_tag="OQM83_004136"
     CDS             371..>789
                     /locus_tag="OQM83_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCS1522225.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELL"
BASE COUNT          219 a          159 c          236 g          175 t
ORIGIN      
        1 ctgtggaact ggattctgtt ccgtaaattt tcctctccgg aacaagtcga aaatgtggcc
       61 gcgcagctgc tggagtttgc gtaatggtgg atttacgtaa agcggcgcgg gggcagatgt
      121 gcaccgtcag aattcctggc tactgcaatc acgatccgga aacgtctgtg ctggcgcatt
      181 accgactggc gggaacgtgc ggaacagcga taaagccaca cgatatgcag gcagcgattg
      241 cctgtagctc gtgccacgat ttaatcgacg ggcgggtaaa aaccagcgat tacaccaaag
      301 aagaattacg cctgatgcat gcagaaggtg tttttcgcac acaagaaatc tggagaaagg
      361 agggatattt atgatttacc caacgaatac aggaaaaagc ggagaacacc ttcgtctcac
      421 cacgctggaa agtgtctgga ttcagggaaa actacgtatg tgggggcgct ggtcgtatat
      481 tggtggcggt aagacgggga atatgtttaa cctgatgttg acctctaaaa agctgacaaa
      541 aacggcaatt aacgaggcgc tccggaggat gaaaaaagca ggtctgaaca agtctgaact
      601 tgaggctttt ttgcgggata tgattaacgg taagcaaaag agctggctgg cgcattgtac
      661 tgatgcagag gcgttatgta ttgatcgggt cataagtgag gtgctggcag agcatccagg
      721 attgattagc gtccttcggc aacggtatga ggggcggggg atgaccaaac gcaaaatggc
      781 tgaactgct
//
LOCUS       DAJVQG010000334          769 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJVQG010000334 DAJVQG010000000
VERSION     DAJVQG010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.340"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..661)
                     /locus_tag="OQM83_004137"
     CDS             complement(<1..661)
                     /locus_tag="OQM83_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HCS1522226.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SV"
BASE COUNT          206 a          214 c          174 g          175 t
ORIGIN      
        1 tgacggataa atcgccctgc agtagtctcg ctccggcaga aacagccgaa ttaaccattt
       61 tgaaagctgt ctgaacggtt cccagcatat cggtcgtccg ggcaataaca tcgttctgaa
      121 taaagtccgg tattcccgcg agatcaaaat cgccgaacat gtcttcaatc aactcatcca
      181 ggaatacgga cgactcctcc agttttctgg cggttgcagc tccggcgacc ggaaatgaca
      241 gttccccgct ctcaacaaac tgaaatgaaa cccggcacat acggccttca gtgccggagt
      301 gagaaacggt cacctgcccg tcaatacagc cctgcatttc accgaactgc ggatggatca
      361 gcgtccccgg cccggcggtt tcaatcgctg taatcagcct gtcgcgctgc tctgcgtaat
      421 catcaccaac gagatacgca ttaatcgtca gccgtcgcgt ggcacgcccg agatcttccg
      481 tgtacggttt gtcacggttt gggtattcat ggacctgaac gcggcgtccg aacgaaccct
      541 cgtcgctctc caccgaaaac ggaacgccac gaaatgaagc gtcgtaaaga ttatcgcgcc
      601 aattgatacc tgaagatgca gaaaagagtg aagaaagaga aggtaaagaa ggtaagtcca
      661 tactgtctcc actataaaaa aaccgccaat ttctggcggc ttatatatta atcatcatta
      721 atatctttca tattcaattt gcaattttcg taggtcactc ccgactcgc
//
LOCUS       DAJVQG010000335          768 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJVQG010000335 DAJVQG010000000
VERSION     DAJVQG010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.341"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..765)
                     /locus_tag="OQM83_004138"
     CDS             complement(<1..765)
                     /locus_tag="OQM83_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCS1522227.1"
                     /translation="MVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVRS
                     IDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTPP
                     GYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETDA
                     KGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQG
                     GYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAAR"
BASE COUNT          191 a          214 c          184 g          179 t
ORIGIN      
        1 ccgggccgcg acaccttcat ccggtacggt tgtcgtggta acaccactga atgtggccag
       61 ccagccaaag ctttccagat gattcattcc ggaaaccagc gcctggtacc cgccttgtga
      121 aagccctgcc agcgcgcgac catcggcatc tttacggaca ttaaaacgct tgctaatcag
      181 cgggataata tcgttcatca gttcgcgatc tgccgcttta gcattcagcg gataaaagac
      241 tttacgtctt tcctgaggca cgaaatcttc gggaataatg cccttcgcat cggtttcggt
      301 atccgggatc accaccagca tcggtttaat tttcccttca gccagcaggt tatccatgat
      361 ttgcgggata cgcccctgat cgatagcgga acgtccggta tcaccaaaac cgtgatagaa
      421 atagagcact ggcaaaggct cgcccatgcc ggtgtatcct ggcggggtcc agacatacat
      481 ctgacgttca gattgcaatg cgttggagtg gtaagttatg gcgatcaaat caccatgcgc
      541 aacagaacgc gtatccagat aactgcctgg caccagaacc atactggagt taacctggcg
      601 ctgaggctta gtcattgcgg tgcctatatc aatgctgcgt acaccatcaa cattgaaaaa
      661 atactcatac aggttgcctt tcaggacagg cgtgcgccac gaccagactc ctgcttcgtc
      721 tttgatcatc gggtgaatat tgtccggaac cggaacaccc accactaa
//
LOCUS       DAJVQG010000336          767 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJVQG010000336 DAJVQG010000000
VERSION     DAJVQG010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.342"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            89..>767
                     /gene="istA"
                     /locus_tag="OQM83_004139"
     CDS             89..>767
                     /gene="istA"
                     /locus_tag="OQM83_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HCS1522228.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQA
                     GDKAMGYSHFCRCYRKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVA
                     IFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRY
                     EPVINDSY"
BASE COUNT          197 a          201 c          186 g          183 t
ORIGIN      
        1 ggaattaact cttttcacca cagtctccat tgccgtgttt aaaccagtgg agacgtcaat
       61 gtccaaaaag agaaaatcca ggagcactat ggaaacttgt tttaagatcc ttcagctcaa
      121 gttcgataag aagctgacta accgttgtat cggactgacg ttacacatca gcgcctcaac
      181 cgtttttgag gttcttgccc gcttcaaagc ctcttctctg tcatggccat tacctgcgga
      241 tatatcccat gacacgctgg agaaactcat tttcccgccc aaagacacct ctgcgtcaga
      301 gcttgttatg cctgacatgc tgtacttcga tacggaaatg agaaaacctg gcgtgacgcg
      361 ccaactgttg tggatggaat acaaggcaca ggcgggtgac aaagcgatgg gatactcaca
      421 cttctgccgt tgctaccgga agtggaaaaa aacgcgacgc ctctccatgc gacaggagca
      481 tcgtgctggc gaaaagctct ttatcgactt ctgtggtcct acggtccctg ttattaatcc
      541 ggacaccgga gagatacgcc gcgtggcaat cttcgtggct gtaatggggg cgtccaacta
      601 tacctatgtt gaagcgtgcg aaggtcagga catgatgtca tggttaaatg cccacagccg
      661 ttgcctgacc ttcctgggcg gcgtaccgaa gctgctcatc cccgataacc tcagaagcgc
      721 agtgaaaaaa gccgatcgct atgagcctgt catcaacgac agttatc
//
LOCUS       DAJVQG010000337          748 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJVQG010000337 DAJVQG010000000
VERSION     DAJVQG010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.343"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..186
                     /locus_tag="OQM83_004140"
     CDS             <1..186
                     /locus_tag="OQM83_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522229.1"
                     /translation="ATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLA
                     QGKRRCQQQFTSQEVQN"
     gene            196..>748
                     /locus_tag="OQM83_004141"
     CDS             196..>748
                     /locus_tag="OQM83_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="catalyzes the transfer of alpha-xylosyl residue;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCS1522230.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNR"
BASE COUNT          192 a          169 c          197 g          190 t
ORIGIN      
        1 gccacgatta gcatcattat tgcgctattc acgattgttc cggcgatctg ttatttgctg
       61 agcgcgatta tcgctaaacg ctactactca ctcacgacgc acaatctgaa aaccgttatg
      121 gaacagctgg ctcagggtaa acgccgttgc cagcaacaat tcacctctca agaagtgcag
      181 aactaaggaa cggcaatgaa aattagcgat ggaaactggt tgattcaacc tggcctcaat
      241 ttgattcacc cgcttcaggt gttcgaggtt gaacagcagg ataatgaaat ggtggtctat
      301 gctgcccccc gtgatgtgcg tgaacgtacc tggcagcttg atacgccttt atttacgctg
      361 cgctttttct ccccacagga aggtattgtc ggtgtgcgga ttgagcattt tcagggggcg
      421 ctgaataacg gtcctcatta tccgctcaat attttgcagg acgtgaaggt cacaatcgaa
      481 aacacagaac gttatgctga gtttaaaagt ggcaacttaa gcgcgcgtgt cagcaaaggt
      541 gagttctggt cactggattt tctgcgcaac ggcgaacgta ttaccggtag tcaggtgaaa
      601 aataatggct acgtgcagga cacgaataat caacgcaatt atatgtttga gcggcttgat
      661 cttggcgttg gcgaaacagt ttacggtctg ggagagcgct ttactgccct ggtgcgcaat
      721 ggccagacgg tagagacctg gaaccggg
//
LOCUS       DAJVQG010000338          742 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJVQG010000338 DAJVQG010000000
VERSION     DAJVQG010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.344"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(1..345)
                     /locus_tag="OQM83_004142"
                     /pseudo
     CDS             complement(1..345)
                     /locus_tag="OQM83_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
BASE COUNT          188 a          207 c          161 g          186 t
ORIGIN      
        1 gaacaggtgg cagtttcgcc ccatcccgta atgtttatcg atgctacggc gcgctgaggc
       61 gctcaaatcg agataatcag ccagttcatc gagggtatag gcttcctctc gccactccat
      121 cacctgccat tccaggtgga acggattttt acaaatcagt ccgctgtagt tcacatcagc
      181 acaaagtttc tcacacagcg aacgttcaat cgcagcggcg tatttcagcg ccttaaccga
      241 gctgtccggc gcgggagcgt tccggtcgct ccagtcaatc gctgccccct cgcgatcaac
      301 gtcaaaaacc agccagaact gcgcatgagg ctggttctgc cggatctgga catgatttca
      361 ggggcgtttg tttttcagtc gcttacacta cacccaacgg caaatcctgt cgaaccttcc
      421 cgaaaacccg atccatttac cataactccc gttgtacgtg ccctctcaga cgctctctga
      481 cgcaaaaaac tcaaaaagac aagaaaaaac agggtaaaca taaaaaatgc gtcagaatcg
      541 tttttagcgc gttgcataac tatgcacgaa agcgacccgc gatcggtttt gtgtgttctg
      601 aataccgaac gggtcaaaat gacacgtttt ctctccctca atggagcgtt ctatacagag
      661 atttttgttc ccctatccat tattctgatt accgggtttc caagctgaaa acaggacttt
      721 tctgcgacca gtttttgcga at
//
LOCUS       DAJVQG010000339          740 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJVQG010000339 DAJVQG010000000
VERSION     DAJVQG010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.345"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..384
                     /locus_tag="OQM83_004143"
     CDS             <1..384
                     /locus_tag="OQM83_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1522231.1"
                     /translation="RLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQPAGKRLFA
                     LSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPESEQKALLSE
                     MRARVENFNKLFEELLKARKRSANK"
     gene            588..>740
                     /locus_tag="OQM83_004144"
     CDS             588..>740
                     /locus_tag="OQM83_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCS1522232.1"
                     /translation="MNTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
BASE COUNT          234 a          179 c          154 g          173 t
ORIGIN      
        1 cgcctcttac ataggcgtaa aaaattaaaa ctgtctcagg ccgcattagg taagctggtc
       61 aaagtggctc acgtaacaat ttctcaatgg gaaagagatg aaacacagcc ggcggggaaa
      121 agattattcg cactgagcca agcgcttcag tgctcaccga cttggcttct ttttggggat
      181 gaagataaac aaccaggcga accgatcccg gataatcagc cagccattct gacagaagat
      241 caaaaagagt tacttcaact gttcgacgca ctgcctgagt cagagcaaaa ggccctgttg
      301 tcagagatgc gtgctcgagt tgaaaatttc aacaaacttt ttgaagaact actcaaagct
      361 cgcaaaagaa gcgcaaacaa ataatcccct tttttctcca caccctgtaa taaaaagcac
      421 aaactttcaa acacttgtgt tttttacatc aaaaaaactt aggtttttct acacaaaaat
      481 cttgaccgcc caccttaggt tattctaaat ttcattcatc aagacaccgc acggtgttct
      541 cagcaaacag ttccgctacc ccggcgttaa ggggaaatgg ggtcaacatg aatactatcg
      601 atcttggcaa caacgaatct ctggtgtacg gcgtgttccc caaccaggac ggcacattca
      661 ccgccatgac gtataccaaa agcaaaacgt ttaaaaccga agctggcgca cgtcgttggt
      721 tgggaagaca ttcaggtgag
//
LOCUS       DAJVQG010000340          737 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJVQG010000340 DAJVQG010000000
VERSION     DAJVQG010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.346"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..232
                     /gene="crcB"
                     /locus_tag="OQM83_004145"
     CDS             <1..232
                     /gene="crcB"
                     /locus_tag="OQM83_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCS1522233.1"
                     /translation="FAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGR
                     FGWALLNVFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(286..495)
                     /gene="cspE"
                     /locus_tag="OQM83_004146"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(286..495)
                     /gene="cspE"
                     /locus_tag="OQM83_004146"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCS1522234.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(670..>737)
                     /locus_tag="OQM83_004147"
     CDS             complement(670..>737)
                     /locus_tag="OQM83_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522235.1"
                     /translation="TYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          200 a          157 c          155 g          225 t
ORIGIN      
        1 attcgcctgg ttcagcagga tgacgaacat tgatccagtg tggaaagtat taatcaccac
       61 cggattttgt ggcggtctaa caaccttctc aacattttcg gcagaagtgg tgtttttgtt
      121 acaagagggc cgctttggct gggcgttact gaacgttttc gtcaaccttc tggggtcttt
      181 tgccatgacc gcactggctt tctggctgtt ttcggcctca accgcacact aaagaccaaa
      241 aaaaacccgc tgattaagcg ggttttgaat tcttgctgac gtatcttaca gagcgattac
      301 gtttgcagca gaagggcctt tggcaccgtt agtgatttcg aactctacgc gctgaccttc
      361 agcaagagtt ttaaaaccat tagtctgtat tgcagagaag tgtacgaaca cgtctttgct
      421 gccgtcttcc ggagtaatga aaccgaatcc tttggactca ttaaaccact taacgttacc
      481 tttaatctta gacatcaaaa ttacctttac atgaaaaata gacacaaatg ctgtgtcggt
      541 taccagtaca ccaattgtgg tacgctttgt ccagtgcaac tttactaaaa agtgataaaa
      601 gtcgcgttat ttttgatgtt acttatttta gttattgatt ttaaagaagt tactaaaact
      661 tcatttgtct caaaactgaa agcgcatcca ggcaaagtac acattgccat tgttgtaggt
      721 acccggaatg taggtca
//
LOCUS       DAJVQG010000341          737 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJVQG010000341 DAJVQG010000000
VERSION     DAJVQG010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.347"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..472)
                     /locus_tag="OQM83_004148"
     CDS             complement(<1..472)
                     /locus_tag="OQM83_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1522236.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGK"
BASE COUNT          241 a          150 c          135 g          211 t
ORIGIN      
        1 ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta acctgcgaat
       61 tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct tgtcctgtac
      121 cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg tcgccgtcgc
      181 cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt gccgggcaat
      241 tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct gaaaatgcat
      301 ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta acttcacaag
      361 tagtgtcgac aatttcaccg acgaagtgaa caacaccatc ttctgccagt gcatgcccgg
      421 ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc ataaaaactc
      481 cattgatata atatatcctt taccatgaat catcacttat attaataagt gtatttttga
      541 ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta attacgcaaa
      601 aataaaatat aaattcattg tttaaatatt atttaaacag taaaaaatca agtttgttat
      661 cagacattaa tgataattca gaatatattt atatagaaag atagataaat ttattaatgt
      721 cagttataaa aaagggc
//
LOCUS       DAJVQG010000342          733 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJVQG010000342 DAJVQG010000000
VERSION     DAJVQG010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.348"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(12..87)
                     /locus_tag="OQM83_004149"
     tRNA            complement(12..87)
                     /locus_tag="OQM83_004149"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52..54),aa:Ile,seq:cat)
     gene            212..718
                     /gene="mug"
                     /locus_tag="OQM83_004150"
     CDS             212..718
                     /gene="mug"
                     /locus_tag="OQM83_004150"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCS1522237.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVTYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT          180 a          169 c          204 g          180 t
ORIGIN      
        1 tttgctatat ctggtggccc ctgctggact tgaaccagcg accaagcgat tatgagtcgc
       61 ctgctctaac cactgagcta aggggccatg gtagcggatt ataaagtaac tccgtgtcgc
      121 aatccagcca ttaccgtgcg cctgctgttt ttataaacaa tgtattttca atccgttata
      181 cttttcctgg tgatgttaga aaggagtaaa tatggttgag gatattttgg ctccagggtt
      241 acgggtcgta ttttgcggta tcaaccctgg gctttcatcc gccgggactg gttttccctt
      301 tgctcatcca gcaaatcgct tctggaaggt gacatatcag gccgggttta ccgaccgtca
      361 gttgaagccg caggaggcac agcatctgct ggattatcgt tgtggcgtca ccaaactggt
      421 agaccgtcca acggtgcaag ccaatgaagt ttcaaagcag gagctgcacg caggtgggcg
      481 taagctgatt gaaaaaattg aagattatca gccgcaggcg ttggcgattc tgggcaaaca
      541 agcatatgaa cagggattca gccagcgcgg tgcacagtgg gggaaacaaa cgctcaccat
      601 tggttcgacg cagatttggg tgctgccaaa tcccagcggt ttaagtcgcg tttcactaga
      661 gaaactggtt gaagcgtatc gcgagctgga ccaggcgctg gtagtgcgtg ggcgataaaa
      721 aacgccaccg aaa
//
LOCUS       DAJVQG010000343          731 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJVQG010000343 DAJVQG010000000
VERSION     DAJVQG010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.349"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..>731)
                     /locus_tag="OQM83_004151"
     CDS             complement(<1..>731)
                     /locus_tag="OQM83_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCS1522238.1"
                     /translation="KAIMVNGPQFGWYAPAYTYGIGLHGAGYDVTGNTPFAYPGLVFG
                     HNGVISWGSTAGFGDDVDIFAERLLAEKPGYYLHNGKWVKMLSREETITVKNGQAETF
                     TVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAKNWQEWTQQAAKQA
                     LTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKGLLPFEMNPKVYNP
                     LSGYIANWNNSPQKDYPASDLFAFL"
BASE COUNT          194 a          195 c          155 g          187 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctgcctgctg tgtccactcc tgccaatttt tggccttcat
      301 ctgatgagtc caggccagca aagacgccac ctctttacca tcccatgcgc gggatttagc
      361 gtaagccgtt tgtgtcgtct ggtcagtttg gagaatgttg ccatgcaccg tacgccagac
      421 agtaaaggtc tctgcctgac catttttcac cgtaatggtt tcctcacggc ttaacatttt
      481 cacccactta ccattatgca agtagtagcc tggtttctct gccaacagcc gttcagcaaa
      541 aatatcgaca tcatcgccga aacctgccgt tgatccccag gaaatcacac cattatgacc
      601 aaaaaccagc ccaggatagg caaatggtgt attgccagtg acatcataac cagcaccgtg
      661 cagaccaata ccataagtat acgcaggcgc ataccagcca aactgcggac catttaccat
      721 gattgctttc g
//
LOCUS       DAJVQG010000344          727 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJVQG010000344 DAJVQG010000000
VERSION     DAJVQG010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.350"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(158..>727)
                     /locus_tag="OQM83_004152"
     CDS             complement(158..>727)
                     /locus_tag="OQM83_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1522239.1"
                     /translation="FFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIV
                     IAGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFF
                     IAVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQ
                     IHLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT          226 a          179 c          157 g          165 t
ORIGIN      
        1 tggcttgata accggataac aacgtgcgct ttgttgatgc cggatgcggc gtgaattcct
       61 tatccggtct acaaaatttg caaatccaaa gcattacatt aagacgtggc ctgataaaca
      121 tatcgcatca ggccattttc gttagctatt gtccacgtca ggctttcaca tcactcactg
      181 caccgaccgg aatatcagca tgaacaggct cgggcggtac tttgcgatat ttatccagca
      241 gatgaatctg gatcctgcgc agcgtgtcgc cattgaggcg atagaaacgg aagtagagaa
      301 tcagcgttac catgaaaaac agagttggta gagcaatcat aataaactgc atacctaata
      361 gggcttgcgt agactgttca acattcggta catagccaat catccctaac acaaccgcaa
      421 tgaaaaaagc cgcaaaggct gaaccgccct tcaccaccat agtctgcacg gagtaagcga
      481 tactttcaca gcgtacgtgc agtttatatt caccgtaatc aacggtatct gccaccatga
      541 tgacctgtaa tacccagaaa agcgccgttc ccacattcag caaaataccc gcaatcacaa
      601 tgaggacgac gttgtgatag ctcattaatg ccattaacag gagaacacca cagcttaaca
      661 ccggaagaat agatgctccg gcccataaaa tgcgtcggga taatgattta actaagcgtg
      721 ggaagaa
//
LOCUS       DAJVQG010000345          726 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJVQG010000345 DAJVQG010000000
VERSION     DAJVQG010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.351"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            35..726
                     /locus_tag="OQM83_004153"
                     /pseudo
     CDS             35..726
                     /locus_tag="OQM83_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
BASE COUNT          167 a          183 c          205 g          171 t
ORIGIN      
        1 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
       61 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
      181 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
      241 tgcacgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
      361 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
      421 agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
      481 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
      541 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
      601 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
      661 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DAJVQG010000346          726 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJVQG010000346 DAJVQG010000000
VERSION     DAJVQG010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.352"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..>726)
                     /locus_tag="OQM83_004154"
     CDS             complement(<1..>726)
                     /locus_tag="OQM83_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001475310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HCS1522240.1"
                     /translation="ELNITTGGIVKARDTQIALNDKSKGDVRVDGQNSLLETFNMYVG
                     TSGTGTLTLTNSGTLNVEGGEVYLGVFEPAVGTLNIGAAHGEAAADAGYITNATKVEF
                     GSGEGVFVFNHTNNSDAGYQVDMLITGDDKDGKVIHDAGHTVFNAGNTYSGKTLVNDG
                     LLTIASHTADGVTGMGSSEVTIASPGTLDILASTNSAGDYTLTNALKGDGLMRVQLSS
                     YDKMFGFTHATGTEFAGVAQLKDR"
BASE COUNT          167 a          205 c          153 g          201 t
ORIGIN      
        1 tctgtctttc agttgggcaa caccggcgaa ttcagtccct gttgcatggg taaagccaaa
       61 catcttgtcg taggatgaca gctgcactcg catcaagcca tcgcctttga gcgcattggt
      121 cagcgtgtaa tctcctgcac tgttcgttga tgcgagaatg tcgagcgtac cggggcttgc
      181 aatggtgact tcactcgaac ccatgcccgt taccccatct gccgtatgag acgcaatggt
      241 caggaggccg tcattgacca gcgttttacc gctataagta ttccctgcat tgaacaccgt
      301 atggcctgca tcatggatca cttttccgtc tttatcgtca cctgtaatca gcatatcgac
      361 ctggtagccg gcatcactgt tattagtatg attaaagaca aaaacacctt cgccagaacc
      421 aaactctact ttcgtcgcgt tggttatata cccggcatct gccgccgcct caccgtgagc
      481 tgcaccaatg tttagcgttc ctacagcagg ttcaaaaaca cctaagtaaa cttctccacc
      541 ttcgacattc agcgtgccgc tattcgtcag ggttaacgta cccgtaccag atgtccctac
      601 gtacatattg aatgtttcaa gaagagagtt ctgcccatcc accctcacgt cgcccttact
      661 tttgtcattg agagctatct gtgtatcacg cgctttaact atacctccgg tggtaatatt
      721 cagttc
//
LOCUS       DAJVQG010000347          709 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJVQG010000347 DAJVQG010000000
VERSION     DAJVQG010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.353"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            131..>709
                     /locus_tag="OQM83_004155"
     CDS             131..>709
                     /locus_tag="OQM83_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCS1522241.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNY"
BASE COUNT          159 a          147 c          169 g          234 t
ORIGIN      
        1 gaaaacgata aaatagcatt cccagggtac ctttatttgc cgccgctgtt agcgaacatg
       61 ctatccttta tgagaattta cttattacgt tgagataatt gccacccgca attgtatgga
      121 cagataatta atgcatgtac accccatttc aactttccgt ttgttccagg aaggtcatct
      181 gctacgtaat agcatcgcta tttttgcgct aaccacgctg ttctatttta ttggtgcaga
      241 gttacggctg gttcacgaac tttctctttt ctggccgctg aatggcgtaa tggcgggggg
      301 gtttgcccgc tatgtctggc ttaatcgact gcattactat gcgatcagtt atgtggcgat
      361 gctggtttat gatgccataa ccaccgaatg gggactggtt tcactggtta tcaatttctc
      421 caatatgatg tttattgtta ccgtcgcctt actggtcgtg cgggataagc gtctggggaa
      481 aaataagtat gagccagtaa gtgccttacg gctatttaat tactgtctga ttgccgcatt
      541 attatgcgct attgtcgggg cgattggttc ggtcagtatt gatagtctgg atttctggcc
      601 tttgcttgcg gactggttca gtgagcaatt ctcaacgggc gtgttgatcg tgcctctgac
      661 ggtcatgctg cttgatatcg actatttcaa aagcattaac gataactat
//
LOCUS       DAJVQG010000348          691 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJVQG010000348 DAJVQG010000000
VERSION     DAJVQG010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.354"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            33..>691
                     /locus_tag="OQM83_004156"
     CDS             33..>691
                     /locus_tag="OQM83_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522242.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     G"
BASE COUNT          188 a          145 c          182 g          176 t
ORIGIN      
        1 cggttatagt aagtcacctg tgagaaaacg ctatgcgcaa atatcgtcta agtgaagaac
       61 agcgagcctt tagttatcag gaagatggca ctaaaaaaag tgtgttatta cggcagatta
      121 tcgccattag cgattttaac gatgtgatag caggaaccgc tggcggctgg atcgatcgcg
      181 aaacggtgtt agcacaggaa ggaaattgct ggatttacga ccagaatgct attgcgttcg
      241 gcggaacggt gatttctggt aatacgcgta tcaccggcac cagcgtgctg tggggagagg
      301 tttacgcaac ggataacgtc tggatcgata atagtgaaat cagccagggt gcatatataa
      361 gtgacagtgt caccattcgt gactcattag tttacgggca atgtagaata tttggtcacg
      421 ccttaattga tcaacattct atgatcgtcg ccgcacaagg cttaacgccc gaccatcaac
      481 tcctgctcca aatttatgat cgcgcaaggg ttagcgcctc gcgtattgtc caccaggcac
      541 aaatttatgg cgatgccgtc gtccggtatg cttttattga acatcgcgcc gaagtttttg
      601 attttgccag tgttgaaggt aatgaagaga acaacgtctg gctatgcgat tgcgcaaaag
      661 tctacggtca cgcgcaggtg aaagctggca t
//
LOCUS       DAJVQG010000349          683 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJVQG010000349 DAJVQG010000000
VERSION     DAJVQG010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.355"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..406
                     /locus_tag="OQM83_004157"
     CDS             <1..406
                     /locus_tag="OQM83_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCS1522243.1"
                     /translation="LDIQANSVIAAMKACDVKRLIFVLSLGIYDEVPGKFGEWNNAVI
                     GEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYELTSRNEPFKGTIVSRKSVAA
                     LITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          233 a          128 c          132 g          190 t
ORIGIN      
        1 tttagatatt caggcaaatt cagtgatcgc tgcgatgaaa gcctgtgatg ttaaacgtct
       61 gatctttgtt ctttccctcg gtatttatga tgaagttcca ggaaaatttg gcgaatggaa
      121 taatgcagtt attggtgagc ccctaaaacc attccgcagg gctgcggatg caattgaggc
      181 ttctggactg gaatatacaa ttcttcgccc tgcctggctg actgatgaag atattattga
      241 ttatgagctt acctcacgca atgaaccatt caaaggtacg atcgtatcac gtaagagtgt
      301 cgcagcatta atcacagata tcatcgataa acctgagaaa cacattggtg agaatatagg
      361 tatcaatcaa cccgtgactg atggtgacaa gccattattc atgtaaacca attaaatagt
      421 taccatcgta atgtactatg tgcagtacga tttattaaac ttattaaggt gaactgatca
      481 atccgatgcg attaacatgt gtagaaccag taagaataaa atacgccatg tatataacat
      541 tacgacttac atctaagtca gcataattct aatatctgca catcaataaa aatgcaaaca
      601 acttccggca aaaaaagtga tacaagatcg atgacatagc gttgaagaaa aggatttgat
      661 aatccgcaaa atttgcacaa cac
//
LOCUS       DAJVQG010000350          676 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJVQG010000350 DAJVQG010000000
VERSION     DAJVQG010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.356"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..543
                     /gene="ipaH9.8"
                     /locus_tag="OQM83_004158"
     CDS             <1..543
                     /gene="ipaH9.8"
                     /locus_tag="OQM83_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCS1522244.1"
                     /translation="TGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTML
                     AEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRT
                     EADRWALAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQ
                     LTDEVLALRLSENGSQLHHS"
BASE COUNT          163 a          186 c          196 g          131 t
ORIGIN      
        1 accggcgctc tgctctccct gggcagggaa atgttccgcc tcgaaattct ggaggacatt
       61 gcccgggata aagtcagaac tctccatttt gtggatgaga tagaagtcta cctggccttc
      121 cagaccatgc tcgcagagaa acttcagctc tccactgccg tgaaggaaat gcgtttctat
      181 ggcgtgtcgg gagtgacagc aaatgacctc cgcactgccg aagccatggt cagaagccgt
      241 gaagagaatg aatttacgga ctggttctcc ctctggggac catggcatgc tgtactgaag
      301 cgtacggaag ctgaccgctg ggcgctggca gaagagcaga aatatgagat gctggagaat
      361 gagtaccctc agagggtggc tgaccggctg aaagcatcag gtctgagcgg tgatgcggat
      421 gcggagaggg aagccggtgc acaggtgatg cgtgagactg aacagcagat ttaccgtcag
      481 ctgactgacg aggtactggc cctgcgattg tctgaaaacg gctcacaact gcaccattca
      541 taatcacatc gcataaacca cagaccggac tgactccgga aaaacagagg cccgcccccg
      601 ggcctccccg gattcatccg tttccctgtt cagcctgaca gcacgccccc gcggccggat
      661 gacagactcc gcttcg
//
LOCUS       DAJVQG010000351          675 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJVQG010000351 DAJVQG010000000
VERSION     DAJVQG010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.357"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>675
                     /locus_tag="OQM83_004159"
     CDS             <1..>675
                     /locus_tag="OQM83_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HCS1522245.1"
                     /translation="DVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYR
                     IMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDC
                     CDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQ
                     FARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYN
                     EWHPHSA"
BASE COUNT          166 a          162 c          208 g          139 t
ORIGIN      
        1 ggatgtgctt ctccgtatac accatgttat cggagagctg cccacgtatg gttatcgtcg
       61 ggtatgggcg ctgcttcgca gacaggcaga acttgatggt atgcctgcga tcaatgccaa
      121 acgtgtttac cggatcatgc gccagaatgc gctgttgctt gagcgaaaac ctgctgtacc
      181 gccatcgaaa cgggcacata caggcagagt ggccgtgaaa gaaagcaatc agcgatggtg
      241 ctctgacggg ttcgagttct gctgtgataa cggagagaga ctgcgtgtca cgttcgcgct
      301 ggactgctgt gatcgtgagg cactgcactg ggcggtgact accggcggct tcaacagtga
      361 aacagtacag gacgtcatgc tgggagcggt ggaacgccgc ttcggcaacg atcttccgtc
      421 gtctccagtg gagtggctga cggataatgg ttcatgctac cgggctaatg aaacacgcca
      481 gttcgcccgg atgttgggac ttgaaccgaa gaacacggcg gtgcggagtc cggagagtaa
      541 cggaatagca gagagcttcg tgaaaacgat aaagcgtgac tacatcagta tcatgcccaa
      601 accagacggg ttaacggcag caaagaacct tgcagaggcg ttcgagcatt ataacgaatg
      661 gcatccgcat agtgc
//
LOCUS       DAJVQG010000352          673 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJVQG010000352 DAJVQG010000000
VERSION     DAJVQG010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.358"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>673
                     /gene="rlhA"
                     /locus_tag="OQM83_004160"
     CDS             <1..>673
                     /gene="rlhA"
                     /locus_tag="OQM83_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HCS1522246.1"
                     /translation="EVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNRNLDH
                     NWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNAEKAM
                     NNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASYQRGS
                     RKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPVMITK
                     HCLRFA"
BASE COUNT          178 a          173 c          180 g          142 t
ORIGIN      
        1 gtgaagtcat cggttttcgt gccaatacgg tcgagaaaac cggagaaaat cagtaccgcg
       61 tctggcccaa tgaaatgcca gcagatttgc acaaaattcg tccacatcac ccactaaacc
      121 gtaatcttga tcataactgg cagcaggcac tgacaaaaac ctccagcgaa cgtcgggtgg
      181 cggtagacat tgaactgggc ggctggcagg aacaactgat tctgaccctc accagtgaag
      241 agggtgtcag catcacgcat acgctggacg ggcagttcga cgaagccaat aacgccgaaa
      301 aagcaatgaa caatctgaag gatggtctgg caaaactggg gcaaaccctc tattacgccc
      361 gcgatgtgca aattaatttg ccgggggcgc tgtttgtacc aaacagtctg ttaaaccagt
      421 tccgccgtga agctgctgac atgctggatg ctgcgcgtct tgccagttac cagcgcggca
      481 gccgtaaacc ggttgctgat cctgcgccgg tttttccgca aacgcatctg agtttcctcg
      541 cgaacgtata caaccagaaa gcgcgtgaat tttatcatcg ctatggtgtg cagctgattg
      601 acgcggcgta tgaggcacat gaagagaagg gcgaagtccc ggtgatgatc accaagcatt
      661 gtctgcgctt tgc
//
LOCUS       DAJVQG010000353          671 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJVQG010000353 DAJVQG010000000
VERSION     DAJVQG010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.359"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(58..261)
                     /gene="tatE"
                     /locus_tag="OQM83_004161"
     CDS             complement(58..261)
                     /gene="tatE"
                     /locus_tag="OQM83_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCS1522247.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(390..>671)
                     /locus_tag="OQM83_004162"
     CDS             complement(390..>671)
                     /locus_tag="OQM83_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1522248.1"
                     /translation="PAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSR
                     IIDPFGVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
BASE COUNT          170 a          194 c          156 g          151 t
ORIGIN      
        1 tttgaagcgg tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
       61 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
      121 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
      181 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
      241 ggtaatacta atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
      301 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
      361 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
      421 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
      481 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
      541 cggctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
      601 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccgcgaacc
      661 caggcggcag g
//
LOCUS       DAJVQG010000354          664 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJVQG010000354 DAJVQG010000000
VERSION     DAJVQG010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.360"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(1..202)
                     /locus_tag="OQM83_004163"
                     /pseudo
     CDS             complement(1..202)
                     /locus_tag="OQM83_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
BASE COUNT          171 a          177 c          146 g          170 t
ORIGIN      
        1 ctcgagaacc cggccagtct tgcgactgcc ctgcacatcc caccataatt tgaatggagc
       61 tgtgcattac gcataaaaaa accgcttcag cgcggctatg cgctcaagta acgttcgggt
      121 ttccacgccc ggcacccgtt ttataaggtg caggtgcact ataattccac ccgttctggt
      181 tttcaatagc tacattcaac attttctctt tcctttcatc accgaagtga actttgttga
      241 tgcggtgcct ggtgcctcca ggtgacgtta accagttaac aattaacgcc ggatacaggg
      301 aaacccataa ccacccgata cgggggaact aatcgctttt taactgtgcc gcgtgcgctg
      361 agccgcattc accgcatcac aaaattcact ttaaaaaggg cggacatcag ccagcaatga
      421 aactgatgcc gccaaaggta ccaatcaaca tggagtgttg tggcgtggtt gtcacttaag
      481 cgtatggtca acctgacaac ccggtgtcct caacggggaa ggaataactc cgccatactt
      541 accgccgcgt catttcgcgt tgtgtgcctg cttttaacca cgtcaggcga ggtggtatcc
      601 ttcttatccc gaataaccaa gaaggaaatc tatatgacta aagaagaatt tgtctcttat
      661 attt
//
LOCUS       DAJVQG010000355          661 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJVQG010000355 DAJVQG010000000
VERSION     DAJVQG010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.361"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(1..661)
                     /locus_tag="OQM83_004164"
                     /pseudo
     CDS             complement(1..661)
                     /locus_tag="OQM83_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
BASE COUNT          209 a           85 c          125 g          242 t
ORIGIN      
        1 atttcatcta aggtcatgtt tttaactttt atattcctct ggtttacaac ttcctctgtc
       61 ttgtaagtgt gtatattata tggggtatat gctattttta ataggatagt gaaacaattc
      121 gttatccaca tctattccgt cagagtgaac ttcaaatgca ctaggcgaaa gaattgccag
      181 gccagtatta agcttaagtg ttggggaaaa caaattaaat aacatgagct gcatagtagg
      241 gcaattgttc ttcccagaaa catttaaaaa atttaagttt ttgctctgtt ttattatggg
      301 aaataaagtt ccaatatcgg atgtattatt agtatcaatg tgggttattt tattgttgtc
      361 cagtcttaaa cttttaatag atggaagaca agtcaggaaa tcaagattta tatttttcaa
      421 ctggttgtta gcagcgttta atgtctgaac attgcagaag ttgctataga atattttaat
      481 ttcagaaagc aggtttttgt taataagtaa cgtattcaag ttgggaaata agacattgtg
      541 taaacttgtt agtttattat gggatagatc cagcagtaag agttaatttt tattacctgc
      601 aacaatatca gataattgat tatggctgag attcaggtat tgcagacatt cgcaagattc
      661 t
//
LOCUS       DAJVQG010000356          659 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJVQG010000356 DAJVQG010000000
VERSION     DAJVQG010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.362"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..190
                     /locus_tag="OQM83_004165"
     CDS             <1..190
                     /locus_tag="OQM83_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522249.1"
                     /translation="KKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHDMN
                     AGFDEKILFPSSVESTAG"
     gene            201..368
                     /locus_tag="OQM83_004166"
     CDS             201..368
                     /locus_tag="OQM83_004166"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522250.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            476..>659
                     /locus_tag="OQM83_004167"
     CDS             476..>659
                     /locus_tag="OQM83_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522251.1"
                     /translation="MDIAVLEIALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLT
                     WQLRKAKPDCKLPDKAM"
BASE COUNT          199 a          131 c          171 g          158 t
ORIGIN      
        1 gaaaaaaatt ggcttttaca ccattggcga taaaagcatt tacctgtatg acctacgccg
       61 catggatgaa atcatggagg ctcttgataa tcgttcgtcg atggattggt gtgttgctgt
      121 tcatgatatg aatgcagggt ttgatgaaaa gattttgttc ccctcatcag ttgaaagcac
      181 tgcgggttaa ggagtaacac atgaccacta ttaccaaaga acgtattgaa ttgttcatta
      241 aaaacccgct tgaaaacggg cttacccgtg gtgaacaaat ggaactggca cggattgcgc
      301 tggcatcgct ggaagcagag ccggttgtgt tctggtttga aaaatatcaa gaaggggcta
      361 cggcatgacg acttttacca gagagcagtt aatagctcac gcagaggaga ctattgaagc
      421 acagagactg tgcataccgg gcacaatcga ccatgacatc atccgcacat ataagatgga
      481 tattgctgtt ctggaaatcg cactggtatc gctggcagca gagccagccg gtaaattgca
      541 tgaatacaaa ccagtgggat atcagcgtct ggtcgatgag ttaaccatgc tggtaaagca
      601 gttaacctgg caactgagga aagcgaagcc agactgcaaa ttaccggata aggcgatga
//
LOCUS       DAJVQG010000357          656 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJVQG010000357 DAJVQG010000000
VERSION     DAJVQG010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.363"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(44..>656)
                     /gene="fabY"
                     /locus_tag="OQM83_004168"
                     /gene_synonym="yiiD"
     CDS             complement(44..>656)
                     /gene="fabY"
                     /locus_tag="OQM83_004168"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCS1522252.1"
                     /translation="EDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHR
                     GDWCAQLQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLA
                     TLTGWGLIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGR
                     KARVQMQVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT          146 a          190 c          169 g          151 t
ORIGIN      
        1 gaccggaatg aatgagagag gcagcaccgc ccctctccat aagctactct tcttcgttcc
       61 cgccctcttc atacgggcca aatggcttcg cgggcagaac gatatacgtg ccttcaaaca
      121 ctgcacccgg cgtctcgtcg ccaaagattt caacttgcat ctgcacccgt gcttttcgtc
      181 cgcgcgccag acggtcgaga tcgccgctta aggcaccaag gtcggctacc gcatgaggtt
      241 taccgctaat cggcttgctg tagcggatat gcgcatccgc cagaataatc gttccgccga
      301 ggtggcgttc acgcagcatc agccagataa gtccccaacc ggtgagcgtc gccagcgaga
      361 ataaactccc ggcaaacaac gtatggtgcg gattctgatt accggtttct ggcatggtgg
      421 tgataaattt ttgcccggta tattgctgaa tgcgcacgcc cattttttcg cttaacggga
      481 tatgttcgta ccacgcctgt tgcagctgcg cgcaccagtc accgcgatgc agaatgtcat
      541 ccagagtggc aacgggttta atcatcaaaa aatggcgaat cggcgtggta gttggggtgg
      601 tgatctcccc ctgattaatg aatcccagct tggcgaaaaa ctccaccgcg tcttca
//
LOCUS       DAJVQG010000358          645 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJVQG010000358 DAJVQG010000000
VERSION     DAJVQG010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.364"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
BASE COUNT          220 a          100 c          130 g          195 t
ORIGIN      
        1 gatgccattt tgcagtagtc tattttataa aaaaattaag taggctaaga agtaccttag
       61 aaatcaattt taaggggtct aaaacgctct gtaacgagct aaactgtata agtggcagta
      121 agatgccttt tagactgaca ctcgcttaga gagcttccta gagcttctgg gaggattcat
      181 tagatatcgt tgtatacaga gacacagtat ctataaaggg tattaagaga ttcttagaag
      241 ttactgtaaa gaaccactaa gataagaaga gagggacaaa gaaccgaagg aaagtccctt
      301 attaagtagg agtcttaata gactgtctta atagataact taaaagtctt aaaggatatc
      361 ttccaagaag agaggaacga agaaccagag gaagtgaccg ttgaccgttt gaacagactg
      421 tttaagtaaa gcctataaag tatctattaa ttaagggcta tgtctcaata ctagcagttt
      481 gtcaatacct ttgtctttag caattcgtgc tatttattaa atatagcact ttttgttaaa
      541 acagagtatg ctttttaacc taaaaccttg tcgcttcgct ctgggtcaca tgttcttggg
      601 ttcttagtga gagttacact atcttctaag gtactcaata aaaag
//
LOCUS       DAJVQG010000359          644 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJVQG010000359 DAJVQG010000000
VERSION     DAJVQG010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.365"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..109)
                     /locus_tag="OQM83_004169"
     CDS             complement(<1..109)
                     /locus_tag="OQM83_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCS1522253.1"
                     /translation="MASVSISYPSCSATDGVVRNCKSTAGHQRYLCSHCS"
     gene            165..644
                     /locus_tag="OQM83_004170"
                     /pseudo
     CDS             165..644
                     /locus_tag="OQM83_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT          158 a          141 c          152 g          193 t
ORIGIN      
        1 gagagcagtg agagcagaga tagcgctgat gtccggcagt gcttttgcag ttacgcacca
       61 ccccgtcagt agctgaacag gagggatagc tgatagaaac agaagccact ggagcgcctc
      121 aaaaacacca tcatacacta aatcagtaag ttggcagcat caccgccgtt gtctacacat
      181 ttattctctc ggtgctgatt taccgggaag taaaatggcg ccatttaccc aaactgattc
      241 tggagtcggt tgttaccact tcgattgtat tactgttgat cggtttttcc gtgggcatgt
      301 cctgggcaat gactaatgct gatattcctt atatgatcag cgacgcattg atggggattt
      361 ctgataaccc actgatcata ttgctgctga ttaatatcgt cttattaatt gtcggcatct
      421 ttatggacat gacaccggct gtactaatct ttacaccgat cttcttgccg attgcacaag
      481 agctgggaat ggacccagta catttcggta ttatgatggt ggccaacctt tgtattgggc
      541 tgttaacacc gccggtgggt agtgcgctat ttgttggttg ttctatctcg ggtgtgaaga
      601 ttcaacattt aatcaaacca ttgctgccat tttatgctag catt
//
LOCUS       DAJVQG010000360          643 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJVQG010000360 DAJVQG010000000
VERSION     DAJVQG010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.366"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            65..>643
                     /gene="tehB"
                     /locus_tag="OQM83_004171"
     CDS             65..>643
                     /gene="tehB"
                     /locus_tag="OQM83_004171"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCS1522254.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATML"
BASE COUNT          186 a          133 c          161 g          163 t
ORIGIN      
        1 tgatgcaggg aaaattgtta gtcagaaccg agcgcgccgt tttaatgaaa gcagaggaca
       61 aagaatgatc attcgtgacg aaaactattt tactgataaa tatgaattaa cccgcacaca
      121 ctctgaagta ctggaagcgg tgaaagtggt taaaccgggt aaaacgctgg atctgggctg
      181 tggcaatggt cgtaacagtc tttacctggc agccaatggt tatgatgttg acgcatggga
      241 taaaaatgcc atgagtatcg ccaatgtaga gcgcattaaa tccattgaaa atctggataa
      301 tttacacacc cgagtcgttg atctgaataa cctcacattt gatagacagt acgattttat
      361 tctttcgact gtggtactga tgttccttga ggctaaaacc atcccagggc tgattgccaa
      421 tatgcaacgt tgcactaaac ctggtggtta caacctgatt gtggcggcga tggataccgc
      481 tgattatcca tgtaccgtcg gcttcccgtt tgccttcaaa gagggagaat tacgtcgata
      541 ttacgaaggc tgggagaggg tgaaatacaa tgaagacgtc ggcgagctgc accgcaccga
      601 cgccaacggt aatcgtatta aactgcgttt cgccacgatg ctg
//
LOCUS       DAJVQG010000361          641 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJVQG010000361 DAJVQG010000000
VERSION     DAJVQG010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.367"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..396)
                     /locus_tag="OQM83_004172"
     CDS             complement(<1..396)
                     /locus_tag="OQM83_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS1522255.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRL"
BASE COUNT          194 a          151 c          102 g          194 t
ORIGIN      
        1 caacctggac catgctaaat tcacaccgtg ctgcgcggcc tgattaaata gtgcatacgc
       61 tttggcataa tccacccgca cctgctcacc gtcgtagtag atacccccca gcgctaacgg
      121 agcttctctg tcaccttgtt tagccgctaa ctcgaaccat ttaatcgctt cctgaacatc
      181 aggtttggta tcataacgag gctgataata ttcccagcct aacaggctct gcgcgtcgat
      241 atctcccgat tcagccattt tatgcagtgt atttataaca tcgggagcag gtttaccacc
      301 gtatatgctg tcatacaatt catgcgcttt ctcgctggcc accacgcttg gtgaaactat
      361 cagcattaca gaaaataata ttcttttagt taacatgtat acctttatgt cctcactgtc
      421 atatatttcc ataataatta accaccaaat atccatttaa tgaataaata cagtgccata
      481 aaggtccaag taataagcac acatttagcc tgaaacatta tcattcatgt caccaaatcg
      541 tttatttagt gagataaaac tagcatattc gtaagataaa aaatgcacat ggcaatcaag
      601 aaatgatatt attacttttt tgatgtaaaa ctatttagtt t
//
LOCUS       DAJVQG010000362          637 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJVQG010000362 DAJVQG010000000
VERSION     DAJVQG010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.368"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            450..602
                     /gene="mokC"
                     /locus_tag="OQM83_004173"
     CDS             450..602
                     /gene="mokC"
                     /locus_tag="OQM83_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCS1522256.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT          202 a          125 c          155 g          155 t
ORIGIN      
        1 acacggaaag tcatatcagc gctaacgggt gaccctaagc cctgataatc atagcttaag
       61 gtcaggattt ctgagggatt aattaggaaa agtaataatt aatttcttgt cagtcggcgt
      121 tatttgttag gacaatattt caaaaataat ccagcttgtc gtcaataatt gcgataaata
      181 gataaattaa agttaaataa ttaaatcatt aaaaaattac gccacaaaaa taggcgaggc
      241 gtcttcagga ttagaatcga taaaacagaa tgggttaaag agagcgatat aatagcggcg
      301 ggtgcttgag gctatctgtc tcaggcatta gctgaacggc agatagagaa aagccccgag
      361 tgatatttta ctatcaaccc gaggccccct atatgctgaa cacatgtaga gtgcctctta
      421 ctgaccgtaa ggtcaaggag aagagagcaa tgaagcagca taaggcgatg attgtcgccc
      481 tgatcgtcat ctgtatcacc gccgtagtgg cggcgctggt aacgagaaaa gacctctgtg
      541 aggttcacat ccgaactggc cagacggagg ttgctgtttt cacggcttac gaatccgagt
      601 aagagcaacg gcggggagtg atccccgcca cttttca
//
LOCUS       DAJVQG010000363          624 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJVQG010000363 DAJVQG010000000
VERSION     DAJVQG010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.369"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>624
                     /locus_tag="OQM83_004174"
     CDS             <1..>624
                     /locus_tag="OQM83_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1522257.1"
                     /translation="ITTPDGRASAFYYNHHSQLTSATGPDGLEMRRKYDESGRLIQET
                     APDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLTVTDCSGYVTRYDHDRF
                     GQVTAVHREEGLSQYRAYDSRGQLIAVKDTQGHEMRYEYNAAGDLTAVIAPDGSRNGT
                     QYDAWGKAICTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLD"
BASE COUNT          153 a          184 c          179 g          108 t
ORIGIN      
        1 gcatcaccac gccggatggc agggcatcgg cgttttacta taaccaccac agccagttaa
       61 cgtcagccac cgggcctgac gggctggaaa tgcgccgcaa atatgatgaa tccggccgtc
      121 tgattcagga aaccgcccct gacggcgata tcacccgcta ccgttatgat aatccacaca
      181 gtgacttacc ctgcgcaacg gaagatgcca ccggcagccg gaaaaccatg acatggagcc
      241 gttacggtca gttgctgacc gtcactgact gttccggtta tgtaacccgt tatgaccatg
      301 accgttttgg tcaggtgacg gcggtgcacc gcgaggaagg gctgagccag taccgcgcat
      361 acgacagccg tggacagtta attgcggtga aagacacgca gggccatgaa atgcggtatg
      421 aatacaacgc cgccggtgac ctgaccgccg tcattgcccc ggacggcagc agaaacggga
      481 cacagtacga tgcgtgggga aaagccatct gtaccacgca gggcgggctg acgcgcagta
      541 tggaatacga tgctgccgga cgggtcatcc gtctgaccag tgaaaacggc agccacacca
      601 ccttccgtta cgatgtactc gacc
//
LOCUS       DAJVQG010000364          607 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJVQG010000364 DAJVQG010000000
VERSION     DAJVQG010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.370"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..209)
                     /locus_tag="OQM83_004175"
     CDS             complement(<1..209)
                     /locus_tag="OQM83_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCS1522258.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEA"
     gene            complement(209..424)
                     /gene="essD"
                     /locus_tag="OQM83_004176"
     CDS             complement(209..424)
                     /gene="essD"
                     /locus_tag="OQM83_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HCS1522259.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          171 a          158 c          127 g          151 t
ORIGIN      
        1 tctgcttcgg tatacgtttt accgggaatg atgtcttttc ctgtatgccc gtaacataca
       61 gtccatacac caactatgtc tttgtaagga ttatgtctca caccttccag accatcgtta
      121 ccacctgggc cagtgattaa cacagatgct atagcaatag ccccgccacc aatagcagca
      181 gcaacagctt ttcgtaatga tggaggcatt atccacctct cgcagccttg cgcttatctt
      241 ctttaatctt gaaataaagg tttgtcaggt acgtcagcag gccaaatacc aggctaccca
      301 gcactccaat tgccgcccac tgtgagggcg tgactttatc tagcagctgt aaaaaccagt
      361 acccggcact acctgctgag gtgccatagg cgacacccgt tgttaactta tccatggatt
      421 tcataacccc acctcgcaga caaagcgggt gtaaattgag ggaatactac gaaacgtaac
      481 agactcggag tcagtgaata actcaggtat tgggttatca gctaatatcg agactcaaaa
      541 aatggaaaaa cccgctcgac ggcgggttta agctgtgtga cgaagtaacc actcttaaca
      601 gcataac
//
LOCUS       DAJVQG010000365          605 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJVQG010000365 DAJVQG010000000
VERSION     DAJVQG010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.371"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            309..>605
                     /locus_tag="OQM83_004177"
     CDS             309..>605
                     /locus_tag="OQM83_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCS1522260.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQG"
BASE COUNT          187 a          134 c          111 g          173 t
ORIGIN      
        1 cattagactt aataataaca ataattgacc gccatttaaa tcaagcatac taaatccaat
       61 aagtgacaaa tctttaattt atagatttca tgaatattta aatattaact gaacgagtaa
      121 ttcattgatt caacatctaa tgactatcca acaaataaat atgcgtgata tataaagccc
      181 tttctttagt ttattttcag aaaaagatac acaaaattta tatttggcat tttcttgtct
      241 ctccgacaga ctgaccgaat tgttatcaat gttaacaaaa aaagaacaat tggttataag
      301 gagagagtat gcgtgtcatc accctggcgg gtagtcctcg ctttccttct cgctccagct
      361 ccttgctgga atatgcgcgg gaaaaactaa atggcctgga tgtagaggtt tatcactgga
      421 atctgcaaaa cttcgccccg gaagatctgc tttacgctcg tttcgatagt ccggcactca
      481 agaccttcac cgaacagctg caacaggctg atggactgat agtcgctaca cctgtgtata
      541 aagccgccta ttccggcgcg ctgaaaaccc tgctcgacct gctgccagag cgcgctttgc
      601 aaggc
//
LOCUS       DAJVQG010000366          602 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJVQG010000366 DAJVQG010000000
VERSION     DAJVQG010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.372"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..>602)
                     /locus_tag="OQM83_004178"
     CDS             complement(<1..>602)
                     /locus_tag="OQM83_004178"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="catalyzes the interconversion of D-xylose to
                     D-xylulose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCS1522261.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTD"
BASE COUNT          148 a          175 c          140 g          139 t
ORIGIN      
        1 gtcggtattt aacagcgttt cgtaaccttc acgaccaccc cacaggacat agttttcacc
       61 gcccaattta tgggttgctt ccatcgctgt aacaacttgc gttgccgccc agctgaagac
      121 ttcaggatct gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa agcagttagc
      181 ggttccccac agcagcttca cgccgctctc ttcttgcttg gctgccagga catcaaccat
      241 ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc tcaggggaaa catccacatc
      301 gtggaagcaa taaaatggca catgtaactt gtggaaaaac tcaaatgcga catctgcttt
      361 acgcttcgcc aacgccagtg cctcaccagg ctgctgccac ggacgattaa acgcccccac
      421 accaaacatg tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg caaaacgcaa
      481 gtgctcttcc atacgcttac ccaacaccag ttcgtcggga ttgtagtgac ggaatgctaa
      541 cgggtttgag gattttgagc cttcataacg aacgcgatcg agctggtcaa aataggcttg
      601 ca
//
LOCUS       DAJVQG010000367          597 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJVQG010000367 DAJVQG010000000
VERSION     DAJVQG010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.373"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..345)
                     /locus_tag="OQM83_004179"
     CDS             complement(<1..345)
                     /locus_tag="OQM83_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1522262.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKF"
BASE COUNT          183 a          110 c          106 g          198 t
ORIGIN      
        1 aaatttaccg attgccagcg gtaaaaaata aaatactgcg gtcggaatac acgctaacgt
       61 caccatacca cgatgataag cattcatatc acgaatatta aaaagcgttt catcgaattt
      121 ctttattaat tcccgcgcct ccgggagcaa cctttttcct gcttttgtta acgtaacctt
      181 gcgagttgta cgctcaaata gttgaatatg taaatcttcc tccatctttt ttattcttcg
      241 cgttaatgca ggttgcgtaa tattgagtaa tttagcggca ttgttaaacg aactggactc
      301 tgccagtatg acaaatgcct tcatacttga taattcatgc ttcattatta ctccggaaaa
      361 tggaagcgac gattttgggt ggctggccgt taaaaatttt aactgcattt agccaactta
      421 aattaatgaa aaaatgttat taatcgttga gctaaagtca ttagagatgc tttgccctta
      481 atgtaaccat atcgcaataa gttatgtttt taaattgagg gcattattat gaaaaaaata
      541 ccctgggtaa tgactccaac ttattgatag tgttttatgt tcagataatg cccgatg
//
LOCUS       DAJVQG010000368          596 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJVQG010000368 DAJVQG010000000
VERSION     DAJVQG010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.374"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..181)
                     /locus_tag="OQM83_004180"
     CDS             complement(<1..181)
                     /locus_tag="OQM83_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HCS1522263.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            425..>596
                     /locus_tag="OQM83_004181"
     CDS             425..>596
                     /locus_tag="OQM83_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HCS1522264.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQS"
BASE COUNT          180 a          114 c          134 g          168 t
ORIGIN      
        1 taatgctacc aacagcactg ataattatgg tgtagattaa aaattgcacc gcatggaata
       61 aaaaaccttt tcttttacga tttccgcggc gaaaccaaaa gctcatcaga atcaatgctg
      121 cgcagctacc gaaaataatg accagattaa gcgcattatt tgtaaacagt tcagcgatca
      181 ttgttttatc aggctcctcc agataattgt cgtcatgccg gaaacccctg gcggggctat
      241 tttaccgcga caattcattc agatcatcaa tagtcaggga aggaagtagc aacattagct
      301 aactaatcaa tgcgttgatt gtaaatccag ctaagaggtg aggttttcag agcagacaac
      361 ggtgaaatgt catggtattg ttacgtttag gtaacaagaa atttgtctgc acaaggatta
      421 catcatgatt atggcgaaac tgaagtcagc gaaagggaaa aaatttctct ttggtttgtt
      481 ggcggttttc attattgcgg cgtcggttgt gactcgcgcg actatcggcg gcgttataga
      541 acagtacaat attccgctgt ctgagtggac gacatcaatg tatgtgattc agtcat
//
LOCUS       DAJVQG010000369          591 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJVQG010000369 DAJVQG010000000
VERSION     DAJVQG010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.375"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>591
                     /gene="dgt"
                     /locus_tag="OQM83_004182"
     CDS             <1..>591
                     /gene="dgt"
                     /locus_tag="OQM83_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HCS1522265.1"
                     /translation="IAKEILSRLKELKLLEAYGLDELTGPFESIVEMSCLMHDIGNPP
                     FGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAALRLRDGEEQLNELRRKIRQDLC
                     HFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWWRGETPETHHYLMKKPGYYLSE
                     EAYIARLRKELNLALYSRFPLTWIMEAADDISYCVA"
BASE COUNT          149 a          131 c          160 g          151 t
ORIGIN      
        1 catcgccaaa gaaattttaa gccgtctgaa agagcttaaa ttgctggaag catacggcct
       61 ggatgaactg accggcccct ttgaaagcat tgttgagatg tcatgcctga tgcacgatat
      121 cggcaatccg ccgtttggtc attttggcga agcagcgata aatgactggt ttcgccaacg
      181 tttgcacccg gaagatgccg aaagccagcc tctgactgac gatcgctgca gcgtggcggc
      241 actacgttta cgggacgggg aagaacagct taacgagctg cggcgcaaga ttcgtcagga
      301 cttatgtcat tttgagggga atgcacaagg cattcgtctg gtgcatacat tgatgcggat
      361 gaatctcact tgggcacagg ttggcggtat tttaaaatat acccgtccgg cgtggtggcg
      421 tggcgaaacg cctgagacac atcactattt aatgaaaaag ccgggttatt atctttctga
      481 agaagcctat attgcccggt tgcgtaaaga acttaatttg gcgctttaca gtcgttttcc
      541 attaacgtgg attatggaag ctgccgacga catctcctat tgtgtggcag a
//
LOCUS       DAJVQG010000370          584 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJVQG010000370 DAJVQG010000000
VERSION     DAJVQG010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.376"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..518)
                     /locus_tag="OQM83_004183"
     CDS             complement(<1..518)
                     /locus_tag="OQM83_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCS1522266.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSS"
BASE COUNT          233 a          130 c           85 g          136 t
ORIGIN      
        1 gaactattgg catgtagcat ctttgtcgag ataaagaaaa cagtccccat cccaagggcc
       61 attaaaatcg gcaagaaaaa ggtcacccac cagaaccctt tcatagagca attaaaacta
      121 aatcttacgc cgttgagact ggtcatcaag gcctgataac gcagtccttt tgctgccata
      181 aatataagca aaaccaacag tgacaaagtc aaaacacagc caataagcgg catatctgct
      241 gacactgtca ttaagattgc gaaatagaaa aaaacaaaaa caagacaact aacaaaaaca
      301 ttcccaccgg taatcccata agaaaatcgt tgtccgttaa cttccatatt agcatagaga
      361 taacgcttac atttcattaa tgcccatggt aaatagatac ctagcgttat aatcgttaac
      421 aacacattca ccacacaaat aagaaaatat ctctcacctg taccatgaaa aacaaacgaa
      481 tgggacggaa catccatatc attaataact tgagccataa aagttccctg aattaaaaaa
      541 tccattgata taatttatat atacagacag taaaatgttc tgcc
//
LOCUS       DAJVQG010000371          582 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJVQG010000371 DAJVQG010000000
VERSION     DAJVQG010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.377"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
BASE COUNT          210 a          110 c           93 g          169 t
ORIGIN      
        1 tttttatgcg gtttcaccgc accccgtgat gtcgccggga agccccggta aaaaataatt
       61 agcattagaa tagttgcgat aagctgcaat aagcagaacc acctttttgg tttaatatgt
      121 ccttacaaat agaaatgggt ctttacactt atctaagatt tttcctaaat cgacgcaact
      181 gtactcgtca ctacacgcac atacaacgga ggggggctgc gattttcaat aatgtgtgat
      241 gcagatcaca caaaacactc aattacttaa cataaatgta taagtcatac ttttgttttg
      301 ggtgtatttc aatatgttaa aaagtttttc gctacgctag caagcaaaaa tgaaacagga
      361 ataatcgaaa tgggatgttg cgcacagtta aaataaatca ccgtaaataa tcatctgcta
      421 taaataatca ctttcatgca ataccagata agctattttt aaacagacac ttaccacaca
      481 acaaactaat taaatacaaa gaaaggcaac aatcaactta atgttaatga aatgttttac
      541 atacattaac cacttgttta atgcggagaa tcagtacact at
//
LOCUS       DAJVQG010000372          573 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJVQG010000372 DAJVQG010000000
VERSION     DAJVQG010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.378"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            53..430
                     /locus_tag="OQM83_004184"
     CDS             53..430
                     /locus_tag="OQM83_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522267.1"
                     /translation="MTNKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKA
                     TLNRSGKRKSSIAGNFTKLGEAITSSGQRVFGGDLSNVNFYGTNTIFDGDTIFLIKSP
                     DSEEWSSQKASKDVEGIVFGGKK"
BASE COUNT          212 a           89 c          113 g          159 t
ORIGIN      
        1 aaaaaccttg tatactttca cctaatcaca gccagaacta tggatctaac caatgaccaa
       61 caaagaaaac aaagataaat atattaacaa gttcaagaaa gagctttcaa aatatataca
      121 accaactgct ggagtggaca ttcaattatt taactctaag gatggtggtg gtgtaattaa
      181 agccactttg aatagaagtg gaaaaagaaa atcatctatt gctggtaatt ttactaaatt
      241 aggtgaggca attacctcga gtgggcagag ggtttttggt ggtgatctat caaatgtgaa
      301 tttttacgga acaaatacga tttttgatgg agatacaata tttttaatta aatcacctga
      361 ttcagaggag tggtcatcac aaaaagcttc caaagacgta gagggcatcg tatttggagg
      421 gaaaaaatga ttaacattac tactaaggat gcactcaagt cagtggtgaa gtaagagatc
      481 tagtaattct tcttgaaact gatggaaaaa cggtgtctga ttatttgcag ccaatccctc
      541 ccaaaaaaga tacacactgg agcatgattt tca
//
LOCUS       DAJVQG010000373          568 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJVQG010000373 DAJVQG010000000
VERSION     DAJVQG010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.379"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
BASE COUNT          155 a          113 c           91 g          209 t
ORIGIN      
        1 acttatcggg tatcagtttt gataggggta tcagacttga taggggtaac agaattctgt
       61 gggggtagtt cactatgacc tacccctagt tcactgtgag ctaccgttac atccaatcat
      121 cttccgatgt aacgtatcgt tactaccgat gtgtaatatc gttacattca ataattagtc
      181 tatgtaatat atcgttacta ccaactttct tacagagagc atttcagata aatgaatgta
      241 acctctcgtt acatccattt gtcacatagc actctgtgta atgtctttct atgtgtgtgc
      301 cataatcccc ctatattccc tgtgtaaatc atagaataat gctctctatt ccctgtgtaa
      361 ttccctacta aaaaatccaa tgaaaagtag cttaaaagtt caattcttac tttcttcact
      421 tttacatcac tttcgcaaag ccttgtgtaa tgcggcttac agagcaccat cagaaattgt
      481 tggctaaaaa ttgttctgtt tgtttctgaa cgagtttctc gtttgtgcaa attttaaaat
      541 ttattttgtt tttatagcct tatttcgt
//
LOCUS       DAJVQG010000374          565 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJVQG010000374 DAJVQG010000000
VERSION     DAJVQG010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.380"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..169
                     /locus_tag="OQM83_004185"
     CDS             <1..169
                     /locus_tag="OQM83_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522268.1"
                     /translation="VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRP
                     AYAAFVQKLFK"
     gene            184..>565
                     /locus_tag="OQM83_004186"
     CDS             184..>565
                     /locus_tag="OQM83_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCS1522269.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLG"
BASE COUNT          166 a          119 c          143 g          137 t
ORIGIN      
        1 ggtcgttggt gataaagatg tcgtaaccgg caaggatatc gccgggctga aaactgagct
       61 tgagcagaaa aaaattaagt ttgattacca ggaatatccc ggcctgaacc atgaaatgga
      121 tgtctggcgg cctgcctatg cagccttcgt acagaaatta tttaaataaa ataaggcatt
      181 aagatgaaat taatcattac cgaagattac caggaaatga gccgtgtcgc ggcacaccat
      241 ctgcttggtt atatgtcgaa gacgcgtcgt gttaacctgg caattaccgc cggtagcacg
      301 cccaaaggca tgtatgaata cctcaccacc ctggttaaag gtaagccctg gtacgataac
      361 tgctatttct ataatttcga tgaaattccc tttcgcggca aagagggaga aggcgtaacg
      421 attaccaatc tgcgtaatct gtttttcacc cctgcgggga tcaaagaaga gaatatccag
      481 aagctcacta ttgataacta ccgcgagcat gatcagaaac tggcgcgtga aggcggcctg
      541 gatttggtgg tgctgggatt aggtg
//
LOCUS       DAJVQG010000375          564 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJVQG010000375 DAJVQG010000000
VERSION     DAJVQG010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.381"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>564
                     /gene="hipA"
                     /locus_tag="OQM83_004187"
     CDS             <1..>564
                     /gene="hipA"
                     /locus_tag="OQM83_004187"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HCS1522270.1"
                     /translation="LLRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNE
                     YYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGL
                     PSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVSQWLIGATDGHAKNFSVFI
                     QAGGSYRLTPFYDIISAFPVLGGTGITS"
BASE COUNT          144 a          123 c          155 g          142 t
ORIGIN      
        1 actgctcaga ataggcaatg actggtgcat tccgaaagga ataacgccga cgacgcacat
       61 cattaaatta ccgattggcg aaatcaggca gcccaatgcg acgctcgatc tcagccaaag
      121 cgttgataat gagtattact gtctgctgct ggcgaaagaa cttgggttga atgttccgga
      181 cgcagaaatc attaaagcgg gaagggtgcg cgcgttagcg gtcgaacgtt ttgacaggcg
      241 ttggaatact gagcgaacgg ttttacttcg cttgccacag gaggatatgt gtcagacatt
      301 cggtttacct tcatcggtga aatatgaatc agatggaggc ccaggcatcg cgcggatcat
      361 ggcgtttttg atggggtcca gcgaggcgct gaaagatcgc tatgatttta tgaagtttca
      421 agtgtcccag tggttgattg gcgcaacgga tggccatgca aaaaacttct ccgtatttat
      481 tcaggctggc ggcagttatc gactcacgcc attttacgac atcatttcag catttccggt
      541 ccttggcggt acgggaataa catc
//
LOCUS       DAJVQG010000376          561 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJVQG010000376 DAJVQG010000000
VERSION     DAJVQG010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.382"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..77
                     /locus_tag="OQM83_004188"
     CDS             <1..77
                     /locus_tag="OQM83_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="binds with UmuC protein to form functional DNA pol
                     V (UmuD'2UmuC); involved in translesion polymerization;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase V subunit UmuD"
                     /protein_id="HCS1522271.1"
                     /translation="PITISSEDTLDVFGVVIHVVKAMR"
     gene            77..>561
                     /gene="umuC"
                     /locus_tag="OQM83_004189"
     CDS             77..>561
                     /gene="umuC"
                     /locus_tag="OQM83_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCS1522272.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQT
                     GG"
BASE COUNT          129 a          119 c          166 g          147 t
ORIGIN      
        1 cgcccattac catcagtagc gaagatacgc tggatgtctt tggtgtggtg atccacgtcg
       61 ttaaggcgat gcgctgatgt ttgctctctg tgatgtaaac gcgttttatg ccagctgtga
      121 gacggtgttt cgccctgatt tatggggtaa accggtggtt gtgctatcga ataatgacgg
      181 ttgcgttatc gcccgaaacg ctgaggcaaa ggcgctgggt gttaaaatgg gcgatccctg
      241 gttcaaacaa aaagatctgt ttcgtcgctg tggtgtggtt tgctttagca gcaattatga
      301 gctttacgca gacatgagca atcgggtgat gtcgacgctg gaagaactat cgccccgcgt
      361 cgagatttac agtattgatg aggcattctg cgatctgaca ggtgtgcgta attgtcgcga
      421 tctgactgat tttggcagag aaattcgcgc aacggtgcta caacgtaccc atcttactgt
      481 tggtatgggg atcgcccaga ccaaaacgct ggctaagctt gccaatcatg cggcaaaaaa
      541 atggcagcgg cagacggggg g
//
LOCUS       DAJVQG010000377          557 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJVQG010000377 DAJVQG010000000
VERSION     DAJVQG010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.383"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>557
                     /locus_tag="OQM83_004190"
     CDS             <1..>557
                     /locus_tag="OQM83_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1522273.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICAGRPDEGIPAVCFKLK"
BASE COUNT          111 a          162 c          159 g          125 t
ORIGIN      
        1 gacgaaaata ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccgcaac cgctgcacga tgcactggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgccgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca caaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctcggtggt cagattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtgat tgcacagtac tatgaattcc tgcgccttgg tcgtgaaggc
      421 tataccaaag tacagaatgc ttcctaccag gtcgctgcct atctggcgga tgagatcgcc
      481 aaactggggc cgtatgagtt catctgtgcc ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaaga
//
LOCUS       DAJVQG010000378          553 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJVQG010000378 DAJVQG010000000
VERSION     DAJVQG010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.384"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..395)
                     /locus_tag="OQM83_004191"
     CDS             complement(<1..395)
                     /locus_tag="OQM83_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1522274.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVA"
     gene            complement(435..>553)
                     /locus_tag="OQM83_004192"
     CDS             complement(435..>553)
                     /locus_tag="OQM83_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCS1522275.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          124 a          143 c          145 g          141 t
ORIGIN      
        1 tgtgccacac gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg
       61 gcaacggctt ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa
      121 tcggcgactg cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca
      181 aaagcggtat tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag
      241 ccctgctcac tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt
      301 tgtgccagtt cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca
      361 tggcgtggat tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt
      421 agatgtttac cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga
      481 atgccggccc gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga
      541 atagcctctt ttg
//
LOCUS       DAJVQG010000379          553 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJVQG010000379 DAJVQG010000000
VERSION     DAJVQG010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.385"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..495)
                     /locus_tag="OQM83_004193"
     CDS             complement(<1..495)
                     /locus_tag="OQM83_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522276.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDSK
                     IYENAWWGFSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAK"
BASE COUNT          158 a          132 c          108 g          155 t
ORIGIN      
        1 tttcgcatca cccggacagt tagaagaggc tacccaccaa gcatttttat ataccgcacc
       61 attaaagacg acataccagg tctgaccgcc ctgctgttta ttccaggcaa ctacagaaga
      121 attagacgga acagtgccct gtgctggtgt agccgaaccg ccattagccg gcgtattgga
      181 tgcaggcgta tttgaatttg aagaagatga gctgccgctc ttttcgcagg aaagtgtatt
      241 gccaaactga ctaatttcag cagctgttgc ggtacgcttt aaacgccacg ggttagttgc
      301 atcatttgct ttcgcttttc ccgggcaatt tgtagaaaaa ccccaccagg cattttcata
      361 aattttgcta tcgaaaataa cctgatattt attaccacct tgttggttat tccatgcttc
      421 cattgccaat gctggcagag cggaacacac cagccccata ccaatcatag atttagtaaa
      481 tatatttaat ttcattataa tcccttgtga cgtaaaaact gcaaaacaaa agtcctacgc
      541 aggaattatt tac
//
LOCUS       DAJVQG010000380          550 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJVQG010000380 DAJVQG010000000
VERSION     DAJVQG010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.386"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..302
                     /locus_tag="OQM83_004194"
     CDS             <1..302
                     /locus_tag="OQM83_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1522277.1"
                     /translation="SQYVSLAYKARLKEAKLLASTGSTGDSYDNAMAEIIKGLYKAEV
                     IHRKSWKNRTEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNDDLAA"
     gene            437..>550
                     /locus_tag="OQM83_004195"
     CDS             437..>550
                     /locus_tag="OQM83_004195"
                     /inference="COORDINATES: protein motif:HMM:NF024418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HCS1522278.1"
                     /translation="MPCKHHGESIVNKVYSLKYCPVTGGLIAVSELARRVIK"
BASE COUNT          163 a          121 c          130 g          136 t
ORIGIN      
        1 gctctcagta tgtgtcactg gcctataagg cgcgactaaa agaagcaaaa ctgctggcat
       61 caacagggag tactggtgac tcgtatgaca acgcgatggc agagatcatc aagggccttt
      121 acaaagcgga ggtaatacac cgtaagagct ggaaaaaccg gacagaagtg gagctggcca
      181 cactcacgtg ggtggactgg tataacaatc gacgattgct ggaaaggctg ggccatatcc
      241 caccggcaga agcagaaaaa gcttattatg cttccatcgg aaatgatgat ctggcagcct
      301 gagttcacag ataaaacact ctccaggaaa cccggggcgg ttcagttcac aaaaacacat
      361 taatgcagta actatatttt cctttctggt gataacgtcg ggttatcatt agcttcttca
      421 gctattttac ttttatatcc cttgtaaaca tcatggagaa tccatagtga ataaagttta
      481 ttctcttaaa tattgccccg tcaccggggg gcttattgct gtctctgaac ttgcccgcag
      541 ggtaataaaa
//
LOCUS       DAJVQG010000381          549 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJVQG010000381 DAJVQG010000000
VERSION     DAJVQG010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.387"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            99..>549
                     /locus_tag="OQM83_004196"
     CDS             99..>549
                     /locus_tag="OQM83_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HCS1522279.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSSQVSIVYEAGPCGYGLYRRLVKSGFDCMVCAPSLIPKKPGERVKTDRRD
                     AIRLVRSLRAGDLSAVYVPGIEDEAFRDLARAWASARDDLRHARQRLK"
BASE COUNT          131 a          131 c          145 g          142 t
ORIGIN      
        1 ctggccaacg tgggatattt aacgcgggtt aacatcgttg tcagagccgc atgggtgagc
       61 tgttgctaca ccccggtatt tccaccacgg aggccagcat gaacaacaat aacacattgt
      121 acgtcgggtt agatgttcac aaagaatcga ttactgtcgc ttatgctatc aattcagaac
      181 ctgttgaact gatgggtaaa attggcacat cacctactga tattcagaat ctttgtaaac
      241 gtctcaggtc aaagtcatcg caggtcagta tcgtttatga agcggggccc tgtggttatg
      301 gactttatcg tcggctggtg aaatccggtt ttgactgcat ggtctgcgcc ccctcactta
      361 tcccgaaaaa accgggggag cgggttaaaa ccgatcgccg tgatgccatc agacttgtgc
      421 gttcactgcg tgcaggagat ctttctgctg tctacgtacc cggcattgaa gatgaggcat
      481 tccgggattt ggcccgggca tgggcatctg cccgcgatga tttacggcat gcaaggcaac
      541 gtctgaaat
//
LOCUS       DAJVQG010000382          542 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJVQG010000382 DAJVQG010000000
VERSION     DAJVQG010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.388"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..>542)
                     /locus_tag="OQM83_004197"
     CDS             complement(<1..>542)
                     /locus_tag="OQM83_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HCS1522280.1"
                     /translation="KIRLIILFLLAALSFIGLFFIINYQLVSERAVKRADSRFELIQK
                     NVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLF
                     SRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFD
                     RPGKKCISFTLRINGKDHDL"
BASE COUNT          168 a          118 c           99 g          157 t
ORIGIN      
        1 acaaatcgtg atctttgccg ttgatacgta gcgtaaaaga gatacatttt ttacccggac
       61 gatcaaagca attgtatgcc ggattccagc tgttgttaga gtcatctgaa gccaccgacc
      121 acggtcgttt cgatgggtta aaatcggcaa aaataacccg ccctgactcg tcgacaagcg
      181 gtccatttac ttgttcctga tgataaacaa cgattttatc attcgccctc cgcgaaaaga
      241 gataatattt attatcatca agtaccagtc ccaccgagtc taaaaatggc atcatttcca
      301 ttttcagaat cacggcgcgt tgaatctcct ctgtattttt taataaatac agtgagtcct
      361 ttaatgtcag ggccgaacgt tcaatatctt taaagaaata gccaacgttt ttctgaataa
      421 gttcaaagcg gctatcggca cgttttaccg cgcgttccga taccagttga tagttaatga
      481 tgaaaaaaag accaataaat gacagtgctg ccaataaaaa taaaattatc agccttatct
      541 tt
//
LOCUS       DAJVQG010000383          542 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJVQG010000383 DAJVQG010000000
VERSION     DAJVQG010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.389"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            56..391
                     /locus_tag="OQM83_004198"
     CDS             56..391
                     /locus_tag="OQM83_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCS1522281.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
BASE COUNT          157 a          119 c          136 g          130 t
ORIGIN      
        1 aggtgccgtg attggcggta cgggtggtgc aattctgggt aaaatgaaat aaggaatgat
       61 gatgaaaaaa tttgctttac tggcgggcct gtttgttttc gcaccaatga cctgggcgca
      121 ggactacaat attaaaaacg gtttgccgtc agaaacctat attacttgcg cagaagccaa
      181 cgaaatggca aaaactgaca gcgcacaggt tgcagaaatt gttgcggtga tgggtaatgc
      241 cagcgttgcc agccgtgatt taaaaattga gcaatctccg gaactgagcg ccaaagtggt
      301 agaaaaactg aatcaggttt gcgctaaaga cccgcaaatg ctgttgatca cggcaattga
      361 tgacaccatg cgtgctatcg gcaaaaaata atatttaata tgttaccgcg tcgatgaatt
      421 cgacgcggta ttttagctaa cggggatcag aacttatatt tcaggcccac catcactgcg
      481 gtgtcattgt agccattatc acccagctgc acgcccacat taccccagac actggccgcc
      541 gg
//
LOCUS       DAJVQG010000384          542 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJVQG010000384 DAJVQG010000000
VERSION     DAJVQG010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.390"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>542
                     /locus_tag="OQM83_004199"
     CDS             <1..>542
                     /locus_tag="OQM83_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="catalyzes the transfer of alpha-xylosyl residue;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCS1522282.1"
                     /translation="YMTNRGYGVLVNHPQCVSFEVGSEKVSKVQFSVESEYLEYFVID
                     GPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTSFTTNYDEATVNSFIDGMAERNLPLH
                     VFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRRLKAKGLKICVWINPYIGQKSPVFKE
                     LQEKGYLLKRPDGSLWQWDK"
BASE COUNT          126 a          137 c          140 g          139 t
ORIGIN      
        1 ctacatgact aaccgtggtt atggggtact ggtcaatcat ccccagtgtg tctcttttga
       61 agtgggatcg gagaaagtct ccaaagtgca gttcagcgtt gagagtgaat atctcgaata
      121 ctttgttatc gacggcccga cgccgaaagc ggtacttgat cgttataccc gctttactgg
      181 tcgtccggcg ctgccgcccg cgtggtcctt cggcctgtgg ctaaccactt catttaccac
      241 caactacgac gaagcgacgg taaacagctt tatcgatggt atggcggaac gcaatctgcc
      301 gctgcatgtt ttccactttg actgtttctg gatgaaagcc ttccagtggt gcgattttga
      361 gtgggacccg ctgactttcc ctgacccgga agggatgatc cgccgcctga aagcgaaagg
      421 gctgaaaatc tgcgtctgga ttaaccccta tatcggtcaa aaatcccccg tctttaaaga
      481 gttacaagag aaaggctatt tactcaaacg cccggacggt tcgctatggc agtgggataa
      541 at
//
LOCUS       DAJVQG010000385          538 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJVQG010000385 DAJVQG010000000
VERSION     DAJVQG010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.391"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            210..407
                     /locus_tag="OQM83_004200"
     CDS             210..407
                     /locus_tag="OQM83_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCS1522283.1"
                     /translation="MSPCGNDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIIPS"
BASE COUNT          135 a          145 c          118 g          140 t
ORIGIN      
        1 ttaaaacacg cccggaaggc gttccttcac gctattaaat ctcccctgcc ggatgcttca
       61 gaatggcatc cggcattacc acagcaaatc cccctgattt agcgataaaa gctctctgga
      121 ttgcgccccc tggaagtcgg gcgcataatt agtgtgctta tcttttcttc ttatgttcac
      181 cgcgcctggc gcaccaacag cggattgcta tgtcaccctg tggaaatgac acccctataa
      241 actggaaacg aaacctgatc gtcgcctggc taggctgttt tcttaccggt gccgccttca
      301 gtctggtaat gcccttctta cccctctacg ttgagcagct tggcgttacc ggtcactccg
      361 ccctgaatat gtggtccggt attgtcttca gtataatacc ttcctaaata cactacctga
      421 gagaagagat gaaaagagct atcagctaca tgaggttttc aactgagaag cagataaaag
      481 gggactctat acgaagacag tctaagctgg tcactgactg gttatcaaag aaccctga
//
LOCUS       DAJVQG010000386          537 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJVQG010000386 DAJVQG010000000
VERSION     DAJVQG010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.392"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(218..>537)
                     /gene="tabA"
                     /locus_tag="OQM83_004201"
     CDS             complement(218..>537)
                     /gene="tabA"
                     /locus_tag="OQM83_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCS1522284.1"
                     /translation="YLISEDMTEPHEARRTEYHARYLDIQIVLKGQEGMTFSTQPAGT
                     PDTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKM
                     LMA"
BASE COUNT          154 a          131 c          102 g          150 t
ORIGIN      
        1 ccaacacgcc aggtatttaa aacttcactt ttttgtttaa aattgtcggc taatgtaatg
       61 tgttgagaat aattcaaatc tgatattttg ctcatgacga actccttgag ttaataagaa
      121 cgtctaagca ttacaaattt tactcacaaa aaaatatcaa aaaacgctct tttcacaaac
      181 tcgccgtaaa cctggagtta tttacggcga aaagtcatca cgccatcagc atcttcacta
      241 ctgctttgcg aacctgggct ggtgcaccca ctgcgcacag cggtttatgc acttcccccg
      301 gataaaacac aacaaaatca ccttcattaa ggataactgt tttctcatca acgccttccg
      361 gcaaaaatgc gatgtcttta tcagctaacc agtcggtatc cggcgtgcct gcaggttgcg
      421 tgctgaaggt catgccttcc tgacctttta acacaatctg aatgtcgaga tagcgggcat
      481 ggtactccgt acggcgagct tcgtgcggct cggtcatatc ttccgagata agataaa
//
LOCUS       DAJVQG010000387          531 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJVQG010000387 DAJVQG010000000
VERSION     DAJVQG010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.393"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            47..>531
                     /locus_tag="OQM83_004202"
     CDS             47..>531
                     /locus_tag="OQM83_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCS1522285.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKLDLYGKVDARHTF
                     SDNPGDDGDETIIELGFKGETQITDQLTGYGQALTKTKASDTEGSDNTYVKLAFAGLK
                     FGEMGSFDYGRNYGVIYDVEAWTDMLPVFGGDSYTWTDNFMAGRANGVATYRNSDFFG
                     LV"
BASE COUNT          154 a          101 c          127 g          149 t
ORIGIN      
        1 acttattgat atattcatga agattataat cacaagggaa tacataatga aaagaaaagt
       61 tctggcaatg ctggtcccag cgttattagt tgctggcgca gcaaatgcgg ctgaaattta
      121 taataaaaat ggcaataaac tggatctgta cggaaaagtt gatgctcgtc atactttttc
      181 cgataaccct ggcgatgacg gcgatgaaac tatcatcgag ttaggcttca aaggtgagac
      241 ccaaattaca gatcagttaa ctggttatgg tcaagcactg accaaaacaa aggctagcga
      301 cactgaaggt tctgataata cttatgtcaa acttgctttt gcaggtctga agttcggcga
      361 gatgggctcc tttgactatg gtcgtaacta tggtgtgatt tacgacgttg aagcctggac
      421 agatatgctt cctgtattcg gcggcgactc ctatacctgg actgataact tcatggcagg
      481 ccgtgcaaat ggggtagcta cttaccgcaa tagtgatttc ttcggtctgg t
//
LOCUS       DAJVQG010000388          530 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJVQG010000388 DAJVQG010000000
VERSION     DAJVQG010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.394"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..>530)
                     /locus_tag="OQM83_004203"
     CDS             complement(<1..>530)
                     /locus_tag="OQM83_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1522286.1"
                     /translation="MEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWFGWSF
                     MIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGYISMA
                     TFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTTLLML
                     YTFISFIALFTVCLHP"
BASE COUNT          179 a          100 c          131 g          120 t
ORIGIN      
        1 tgggatgcag gcagacggta aacagagcaa taaatgagat aaaggtgtac agcatcagta
       61 atgttgttgg gcgaacggta ttacgaatga gtggagcggt aataaataca caaagcagcg
      121 aacccacggt gtagatactg agtagtttga tcgacatagt gtatgacatg cctgcaacaa
      181 attgtccgta ctgtgccagc cactggctaa ccagataaaa cgttgccatt gagatatagc
      241 catataaggt ataactggct aaatcgataa ttgaacagcg atgttccgta gagctggtgg
      301 ttttctttat gacaggtaag cgacgacccg gatggggtgg gaacgtacaa cggtataaaa
      361 acagagcgtt aataaacata atgcctgcag caatcataaa ggaccaaccg aaccacagtt
      421 cagcccacac taacaggcta atgattagcg gtaataaaaa ttgtccgctg gaaacaaatg
      481 ctttaattaa aatattggct gtcccaggtg agcgtggaaa agcttccatc
//
LOCUS       DAJVQG010000389          522 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJVQG010000389 DAJVQG010000000
VERSION     DAJVQG010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.395"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            1..292
                     /locus_tag="OQM83_004204"
                     /pseudo
     CDS             1..292
                     /locus_tag="OQM83_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079690.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
BASE COUNT          169 a           94 c          117 g          142 t
ORIGIN      
        1 tgaaggtgcg ctttctgaaa tcaacaataa cttacagcgt attcgtgaat tgtcagtaca
       61 ggccactaat ggtacaaact ctgactccga cctgaattca attcaggatg aaattacaca
      121 acgccttagt gaaattgatc gtgtttctaa ccagacacaa tttaatggtg taaaagttct
      181 ggcttctgat cagactatga aaattcaagt aggtgcgaac gatggtgaaa ccattgagat
      241 tgcccttgat aaaattgatg ctaaaacctt ggggcttgat aactttagcg tagcactagg
      301 aaaagttcca atgtcctctg cggttgcact taagagcgaa gccgctcctg acttaactaa
      361 ggtaaatgca actgatggta gtgtgggagg tgctaaagca ttcggtagca attataaaaa
      421 tgctgatgtt gaaacttatt ttggtaccgg taatgtacaa gatacaaagg atacaactga
      481 tgcgaccggt actgcaggaa caaaagttta tcaagtacag gt
//
LOCUS       DAJVQG010000390          521 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJVQG010000390 DAJVQG010000000
VERSION     DAJVQG010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.396"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..396)
                     /locus_tag="OQM83_004205"
     CDS             complement(<1..396)
                     /locus_tag="OQM83_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCS1522287.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADG"
BASE COUNT          148 a          131 c          115 g          127 t
ORIGIN      
        1 cccatctgct tttaaccgct ttcccagtgt cggatcaacg cggccttcta tgtcgtggat
       61 ctcatagcgc caaagacctt tatttgacac aaaacgcagc gccactgctt tatcggcttt
      121 accctttgga ggtgtcaata aacagtgtcc tgccgttaat gccagattgg gtgcaatcag
      181 cgtcgccgta cataaattgc cgctggccgt ttccagttgc ccaaccgcat cccacggtga
      241 ttgggtcgtg tcattcactg gcacacgatc atcatgacca aaaaacaggg tgctgacctc
      301 atcgtttgcc gatttggcaa cgtctggttt atctgcaaac acaaaagcag acgtcaaact
      361 aattgcaccc aacactacag caatggttgt acgcatatca cactctggtg ggtaattatg
      421 attattaaaa gcaaaccctc ataaatacta tagacgggac gggatgaaag tgggagtaaa
      481 atcagataac tacagtcagg aaagataaaa acgggcggca a
//
LOCUS       DAJVQG010000391          521 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJVQG010000391 DAJVQG010000000
VERSION     DAJVQG010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.397"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            30..>521
                     /gene="mpaA"
                     /locus_tag="OQM83_004206"
     CDS             30..>521
                     /gene="mpaA"
                     /locus_tag="OQM83_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCS1522288.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIE"
BASE COUNT          112 a          146 c          141 g          122 t
ORIGIN      
        1 ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg
       61 gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct
      121 ggtttcctgc ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg
      181 atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gacgttgaca ccttctttgc
      241 gccgccatca tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca
      301 atgctaatgg tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa
      361 cggtttatcg ctggaacagc gccgctgaag aacgtgatgt cgtattactg acaggtgaca
      421 aacctggctc agaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg
      481 cctgggtggt ctcattccac gatccactgg cctgtattga a
//
LOCUS       DAJVQG010000392          520 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJVQG010000392 DAJVQG010000000
VERSION     DAJVQG010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.398"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>520
                     /locus_tag="OQM83_004207"
     CDS             <1..>520
                     /locus_tag="OQM83_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS1522289.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLA"
BASE COUNT          138 a          121 c          117 g          144 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc
//
LOCUS       DAJVQG010000393          520 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJVQG010000393 DAJVQG010000000
VERSION     DAJVQG010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.399"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            284..388
                     /locus_tag="OQM83_004208"
                     /pseudo
     CDS             284..388
                     /locus_tag="OQM83_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          168 a          104 c          107 g          141 t
ORIGIN      
        1 atcatacaga taattactgg ttaaccctat atagggcatt ttaaagtgtt gtgatcttac
       61 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacaaga
      121 caaaacagtt tcaaccagta aaaaaacgta aacaccattc gcatgaattt cgtagttaag
      181 atctgaagct tgcagaatgc atcggtgtta ctgccggagt ccgtgaactt agtctgcatg
      241 aatcaccaca gctttacatc tgtcgcagta aacagcaaaa tcagcaaact tacttaatta
      301 tattgagtgc gattacaatc gctggcgatg gtacagttgg tgtggcggtc tcagtccgga
      361 acaatttgaa aatcagaatc tcacttaaag cggcatccat tttacaaaag atggccgtct
      421 gtgtatttgt aatgaatacc ttctacatgt ttagagtgca taatccacga caaggcgata
      481 tcctaaaaat tagctagcag gcaactttga tggcatagat
//
LOCUS       DAJVQG010000394          518 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJVQG010000394 DAJVQG010000000
VERSION     DAJVQG010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.400"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..>518)
                     /locus_tag="OQM83_004209"
     CDS             complement(<1..>518)
                     /locus_tag="OQM83_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCS1522290.1"
                     /translation="GMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVS
                     CLVTLELTGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMST
                     RDGMTGVYNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIV
                     ALTRQLQITLRG"
BASE COUNT          140 a          151 c          121 g          106 t
ORIGIN      
        1 accgcgcagg gtaatttgta actgtcgggt aagcgccaca atcgcttcat cgcccacatc
       61 atgaccccag gtatcgttga tgctcttgaa atggtcgata tcgataatca gtaacgttgc
      121 atcgcgatta tgccgccgac agttatcaaa ttcattgcgt aacatagttt cccaatgacg
      181 tcggttatac acgcccgtca tgccgtcgcg agtactcatg acctgcaacc tgcgtttatg
      241 ttccgccagt ttggttgccg tctggtagct gacccagcca aacagcagag gataaacgac
      301 aataatggga agggagagcc accattccag cggcgcacta ttgaacgaca cggtaatgcc
      361 cgtaagctcg agggtgacaa ggcaggaaac caccatcaac accagacccg cgacaaacag
      421 acgggggccg cctgccccca tcaaattcag acacataatc atcaacatag cggtggaagg
      481 cagcacgttt acgcccatta cgcctaccca cattcccg
//
LOCUS       DAJVQG010000395          514 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJVQG010000395 DAJVQG010000000
VERSION     DAJVQG010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.401"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..501)
                     /locus_tag="OQM83_004210"
     CDS             complement(<1..501)
                     /locus_tag="OQM83_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCS1522291.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLV"
BASE COUNT          143 a          163 c          115 g           93 t
ORIGIN      
        1 caccagaccg gcgtcggtgt agcccaacgc accacaggcc attgcgctga taaagttgtt
       61 ggcaactgtc ggcagataca gtccaggcgt ggtagcttct tcagggtgag atccgcgcca
      121 taatcccgca gtttgccagg cggcataagc caactgaacc acgacaccaa aactgaacag
      181 gcacaccgcc agtgggcgaa accacggaac aaaaccaatc gccaccagca tcgtcgttgc
      241 cggaaacaaa ctcacaaaac tgctcagcac tggatggcga acttccgcca gcacgctatg
      301 cggaaagcgt atgagtcggg taataaatgc gctagttaat aatccccaga tgatcatcgc
      361 cagaatcacc agcccatccc ctaaccagtg gctgacctgc caaacctggc tggcatagcg
      421 ccaggcaaat cccatcccta tcgtccccaa cacaatacca aagtagcctg ccggcaaatt
      481 gagcacttta tcgctctgca tcttcttact catt
//
LOCUS       DAJVQG010000396          509 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJVQG010000396 DAJVQG010000000
VERSION     DAJVQG010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.402"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>509
                     /locus_tag="OQM83_004211"
     CDS             <1..>509
                     /locus_tag="OQM83_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1522292.1"
                     /translation="DVPAKARNIAMVFQNYALYPHMTVYDNMAFGLKMQKIAKEVIDE
                     RVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDEPLSNLDAKLRV
                     QMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGAPKTVYNQPANM
                     FVAGFIGSS"
BASE COUNT          126 a          121 c          154 g          108 t
ORIGIN      
        1 tgacgttcca gccaaagcac gcaatatagc gatggtgttc cagaactacg cgttgtatcc
       61 gcatatgacg gtttacgaca acatggcgtt tggcctgaag atgcaaaaaa tcgccaaaga
      121 ggtgattgat gagcgggtga actgggcggc gcaaattctc ggcctgcgtg agtacctgaa
      181 acgcaagccg ggcgcgcttt ccggcgggca acgccagcgt gtggcgctcg ggcgggcgat
      241 tgtgcgcgaa gcgggcgtgt ttttaatgga tgaaccgctc tctaaccttg atgccaagct
      301 gcgcgtgcaa atgcgcgcag agatcagcaa gctgcaccag aagctgaata ccaccatgat
      361 ctacgtgacc cacgatcaga ccgaagcgat gaccatggcg acgcggattg tgattatgaa
      421 ggatgggatt gttcagcaag ttggtgcgcc gaaaacggtt tataaccaac cagcgaatat
      481 gtttgttgcc ggatttattg gatcatcgg
//
LOCUS       DAJVQG010000397          507 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJVQG010000397 DAJVQG010000000
VERSION     DAJVQG010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.403"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
BASE COUNT          168 a          108 c          125 g          106 t
ORIGIN      
        1 gatcatgcag gcattcatgg acatggcaaa agccgagagg aaagaagagc gccggcgtaa
       61 gcgagcggaa gcaaaagcga agtagccatt acaaagccca tttacgggtg ggcttgataa
      121 tgaaaccgga atttattctg ggccaccagt taacggcagt accacgaaac aacccaagcc
      181 agtaagtggg gaaataacac cggcagccac tgaaagatga acctcctgcc ttatggcaaa
      241 aaagattctt tgtggtggcg gactgatgga aagacatccc aatttcagcc aaacattgaa
      301 ggagttgtta tgtcagcaga aggtttcaat aacccatcaa aattccggga tgagtgggat
      361 agcagcgtaa agagtaagtg atgccatcac aaaagccatt ccctacagag tggctttgat
      421 aatggcttat accctacacg ggataactta actgatatcc cttttaaagg ataaaggtat
      481 tcaagcctga cacatcatgc gctgtat
//
LOCUS       DAJVQG010000398          506 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJVQG010000398 DAJVQG010000000
VERSION     DAJVQG010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.404"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            284..506
                     /locus_tag="OQM83_004212"
                     /pseudo
     CDS             284..506
                     /locus_tag="OQM83_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079803.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
BASE COUNT          155 a          119 c          110 g          122 t
ORIGIN      
        1 ccaccaagta caagataccc aagggaatcc tgtacctaat tcatttgctg ctaaaacatc
       61 agacggcacc tacattgcag taaatgtaga tgccgctaca ggtaacacgt ctgttattac
      121 tgatcctaat ggtaaggcag ttgaatgggc agtaaaaaat gatggttctg cacaggcaat
      181 tatgcgtgaa gatgataagg tttatacagc caatatcacg aataagacgg caaccaaagg
      241 tgctgaactg agtgcctcag atttgaaagc cttagcaacc acaaatccat tatccaaatt
      301 agacgaagct ttggcaaaag ttgataagtt gcgcagttct ttgggtgcag tacaaaaccg
      361 tttcgactct gccatcacca accttggcaa caccgtaaac gacctgtctt ctgcccgtag
      421 ccgtatagaa gatgctgact acgcaaccga agtgtctaac atgtctcgtg cgcagatcct
      481 gcaacaagcg ggtacctctg ttctgg
//
LOCUS       DAJVQG010000399          506 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJVQG010000399 DAJVQG010000000
VERSION     DAJVQG010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.405"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..188)
                     /locus_tag="OQM83_004213"
     CDS             complement(<1..188)
                     /locus_tag="OQM83_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1522293.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVRVRQHERDTGGGDG"
     gene            242..445
                     /locus_tag="OQM83_004214"
                     /pseudo
     CDS             242..445
                     /locus_tag="OQM83_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          126 a          134 c          117 g          129 t
ORIGIN      
        1 ccatcaccgc ccccggtatc ccgctcatgc tggcgaaccc ggacacgcag agtctccggc
       61 gtacagccaa tctttggggc aatggaacaa attgccgccc actgtgagtc atattcatcc
      121 tgactttcca gaaccatacg aatcgcccgc tgacggactt cgggggaaaa acgagtattt
      181 ttagtcatcc tgtttacctc tttctcaggg agtttagtct ccaggatttc cggggcggtt
      241 cagacatcta gaatttactg tcagcaccgg cgttaaagtt tacttctgcg atcctcagag
      301 tccttggcag cggggaacaa atgagaatac aaatgggcta attcggcagt actttcctaa
      361 aaagacatgt cttgcccaat atactcaaca tcgagtaggc agcctgacgg ctgcggcttg
      421 tcatggtctg caattaccgt tataaaaaca ggcaatgtca ttgtctttca ggtggttata
      481 tggcccgttc agctaaaccc cgtaaa
//
LOCUS       DAJVQG010000400          506 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJVQG010000400 DAJVQG010000000
VERSION     DAJVQG010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.406"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(251..463)
                     /locus_tag="OQM83_004215"
     CDS             complement(251..463)
                     /locus_tag="OQM83_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCS1522294.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT          128 a          143 c           94 g          141 t
ORIGIN      
        1 ctcatgttat tctccatatc agataaatag catctgtatt tcaggcatcc tgaatattgc
       61 agcacacact gaataacacc ttattttgtg aaattaatca caaaatataa aatcacgact
      121 taatacggaa aaaactctgt gctactcaga actgtccggt catactgccc gaccggatat
      181 taaaaaaagc aaatctcata aactcctttc agccttctga ctcctggagt aatgaaggct
      241 gcttttttcg ttacaccctt ccccggcagc gccggtggca gtgttcagcc ctttttcggg
      301 caaactgctg ccagtctgtc ctgggcgcaa cacaggcagc ccgtcgccat gcttctgccg
      361 cttccatcca gcgtccactg acttccagaa taacggcccg cccgccggca cgctggtaac
      421 gccagacctg gccgttccgt gaactgtttc tgaatccatt cattttcttg tcccccttta
      481 gttgtcagta ttattgtgat aaataa
//
LOCUS       DAJVQG010000401          506 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJVQG010000401 DAJVQG010000000
VERSION     DAJVQG010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.407"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            213..>506
                     /locus_tag="OQM83_004216"
     CDS             213..>506
                     /locus_tag="OQM83_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522295.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFY"
BASE COUNT          162 a           95 c           84 g          165 t
ORIGIN      
        1 tattaacttc gctctccact taacttttta gttaaggaga gcgaaataat atcaaagtag
       61 cagtaaaacc tataacgtaa attcaaattg ttaaattaac tccctccagt acacaatact
      121 tctcacgtta gttatgagcg atttctgata gtgcctggtt taatcagcgc tttattatct
      181 gcgacgttta tttttattta agagagatcg cgatgatatc attaaaagct ccgcacaata
      241 atttgatgcc atatacgcaa caaagcatac ttaatacggt taaaaacaat cagttaccag
      301 aggatatcaa aagctcccta gtttcctgtg tcgatatatt caaggttttg attaaacaat
      361 attatgatta tccttatgat tgtcgtgatg atttagtcga tgacgacaaa ctcattcatc
      421 tcatggctgc cgtacgagac tgtgagtggt cagacgataa cgcactcacc ataaatgtgc
      481 agtttaatga tttcccagga ttttat
//
LOCUS       DAJVQG010000402          504 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJVQG010000402 DAJVQG010000000
VERSION     DAJVQG010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.408"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..428)
                     /locus_tag="OQM83_004217"
     CDS             complement(<1..428)
                     /locus_tag="OQM83_004217"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCS1522296.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREG"
BASE COUNT          151 a          110 c          105 g          138 t
ORIGIN      
        1 ccttcacgaa catatttaaa cagtgcttta gtcagcggag gcagaatagt gttgatatat
       61 tcgtcagtgg tctgcttgat cttattagca atatcttcag cagcacccag cgcatattca
      121 aagaagctaa tctgtaaacg caggtcattc aggcttacat cgagagtgga atacgtatta
      181 gcgaacgcgt acaacggcag gttctcgttc attttgctaa tttcttcgca cagctcgaga
      241 ttatatttat cccagtcaaa aataacgccg cacagacgcg cattgttttc gatcagtttt
      301 aataagtcgt cacggtcgtt cgggtaaaca atctggaagt tcagacgttc aagcgcgcga
      361 tgaagttcac ggatgggttc ttctttaaaa taaaccccca tgtgattcaa tattgcaata
      421 acgttcatag tcatatctcc aggtaaaaaa ggcccctccc aacacatggg acaaaatgaa
      481 aggaggagcc tcggaaaata cttt
//
LOCUS       DAJVQG010000403          504 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJVQG010000403 DAJVQG010000000
VERSION     DAJVQG010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.409"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(1..500)
                     /locus_tag="OQM83_004218"
                     /pseudo
     CDS             complement(1..500)
                     /locus_tag="OQM83_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
BASE COUNT          190 a          128 c           97 g           89 t
ORIGIN      
        1 gcggttaatg ccaaaagaaa taacaccagt aaggccccgc cctggatatt catggatggc
       61 aacattaata actaggcggc atgactcaag gcgaaaacgg tcattatcca gccccaaaaa
      121 atggcgctca cggtatgcag aaaaccggag gggtcaccca caaaaactcg ccaggcggct
      181 aatatcagaa acccagccaa aggtataaat aacagaaaca gctcaaagca attaaaaccg
      241 ataagcaaat agttaagagg aataacccaa tataaccacc gcggaaaatg tacggatata
      301 agcatacaat attctttcaa tgccagaaaa cttatcagcg caaaaaacgc caacgtcatc
      361 cagcgtggac ctgaaatcac cagtgaaaaa caaatgacga tgccccacca ggtacgaata
      421 cgtaatgtaa cctcaccccc ttttctctcc ggtaaccgcc atagcagaaa acggtttatc
      481 agcgttgcta aaattaacaa cgcg
//
LOCUS       DAJVQG010000404          504 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJVQG010000404 DAJVQG010000000
VERSION     DAJVQG010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.410"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            224..>504
                     /locus_tag="OQM83_004219"
     CDS             224..>504
                     /locus_tag="OQM83_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1522297.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSL"
BASE COUNT          172 a           96 c          107 g          129 t
ORIGIN      
        1 gcttaaagat tttaacgatc gcttattgta aaaataatag caaagaaaat tgtaagatac
       61 agaccgtaaa taaaaacatc ttccttggtc aggaaagaaa tcttgaaagc atatctggct
      121 acgacaaaat cgttgtcaaa tataataact ggatcaccaa agataatggc gagtttgaac
      181 tggcggtcca ttaattatca ttgtaataaa cgaaggaata gtaatgaaaa aggtatttgc
      241 aaaatctctt ctggtcgcag cgatgttttc tgttgcaggc tccgcattgg cagtacaaaa
      301 ggatattact gtaacggcca acgttgatgc cgctctggat atgacacaga ccgataacac
      361 cgcgttgccg aaagcagtag aaatgcaata tctgccgggg cagggtctgc aatcttacca
      421 gttgatgacc aaaatctggt ctaacgacgt taccaaagat gtaaaaatgc agttagtttc
      481 cccggcgcaa ctggttcaaa gcct
//
LOCUS       DAJVQG010000405          500 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJVQG010000405 DAJVQG010000000
VERSION     DAJVQG010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.411"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>500
                     /locus_tag="OQM83_004220"
     CDS             <1..>500
                     /locus_tag="OQM83_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCS1522298.1"
                     /translation="RQIYQNYKSDASTPYFGETGERAITSARIQQALTLNDNLTLPGN
                     IGWLDYDPVCDCQDFGDLVLESVAITQTDADHADAVVRFRIFKDDKEKTTQTLKMVAE
                     NGRWVIDDIVSNHGSVLQAVNSENEKTLAALASLQKEQPEAFVAELFEHIADYSWPWT
                     WVVSDS"
BASE COUNT          134 a          106 c          131 g          129 t
ORIGIN      
        1 cggcagattt atcagaacta taaatcagat gccagtacac cttatttcgg tgaaaccgga
       61 gagcgggcga taacttcagc gcgtattcaa caggcgctta ccctgaacga caatcttacg
      121 ctgccgggca atattggctg gctggattat gatccggttt gtgattgtca ggattttggc
      181 gatctggtgc tagaaagcgt tgcgataacc caaactgacg ccgatcatgc cgatgccgtt
      241 gtgcgctttc gtatctttaa agatgataaa gaaaagacca cgcagacact gaaaatggtg
      301 gcggaaaatg gtcgttgggt cattgacgat attgtcagca atcatggcag cgtcttacaa
      361 gcagttaata gcgagaatga aaaaacgctg gccgctttag cttcgttgca aaaagaacag
      421 ccggaagcct ttgttgccga actctttgaa catattgctg attatagctg gccgtggacg
      481 tgggtggttt ccgactctta
//
LOCUS       DAJVQG010000406          499 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJVQG010000406 DAJVQG010000000
VERSION     DAJVQG010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.412"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..>499)
                     /locus_tag="OQM83_004221"
     CDS             complement(<1..>499)
                     /locus_tag="OQM83_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS1522299.1"
                     /translation="YDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSD
                     SRMLPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDL
                     IVTVEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYG
                     LNNYLT"
BASE COUNT          148 a          114 c          116 g          121 t
ORIGIN      
        1 gttagatagt tattcagacc gtagtagtag ctggcctgaa ataaattagg ctcatcctga
       61 atatcatctt ttaagacctg gccgccgcta atatcccaac gtccaacgcc agggcgtaac
      121 atttgaacga ccgatgaaaa aggttgcgag aatgtccgct ttgagccatc ggattcttcg
      181 acagtaacaa taagatcgct gccgtaacct gacggactca gatcatcaat gacgaaagcg
      241 cctggcggca cagtcgtttc ataaatctta tagccacctt gcgtaatagt gactttggcg
      301 ttggtattag caacgccatg aatgataggc gcaaaactcg ctaatgtcgg aggcaacatg
      361 cggctgtcgc tgtataaacg aatgccgcgg atactgacag aatcaaaggt ttcgcccgtt
      421 gtataagact caccaagaat gagttgagaa cgcagcgagg cgatatcacg ctgtatatac
      481 cgattcttaa aatcataat
//
LOCUS       DAJVQG010000407          493 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJVQG010000407 DAJVQG010000000
VERSION     DAJVQG010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.413"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            3..>493
                     /gene="bluF"
                     /locus_tag="OQM83_004222"
     CDS             3..>493
                     /gene="bluF"
                     /locus_tag="OQM83_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HCS1522300.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDS"
BASE COUNT          136 a          108 c          107 g          142 t
ORIGIN      
        1 taatgcttac cacccttatt tatcgtagcc atatacgtga cgacgaacct gtcaaaaaaa
       61 tcgaagaaat ggtttcgata gcaaatcgca ggaacatgca gtctgacgta acagggatct
      121 tactgtttaa tggttctcat tttttccagc ttctggaagg tccggaagaa caggttaaaa
      181 tgatatatcg ggctatatgc caggatccac ggcactataa tattgttgag ctgctgtgcg
      241 attacgcgcc tgctcgccgt tttggcaaag cgggaatgga attatttgat ttgcgcctgc
      301 acgagcgaga tgacgtttta caggccgtat tcgacaaagg cacatcaaaa tttcagctaa
      361 cttatgatga cagagcgcta caattttttc gtacttttgt ccttgcaacc gaacaatcaa
      421 cctatttcga gatccctgcc gaagactcct ggctttttat cgctgacgga tctgataaag
      481 aacttgattc ctg
//
LOCUS       DAJVQG010000408          493 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJVQG010000408 DAJVQG010000000
VERSION     DAJVQG010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.414"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..185)
                     /locus_tag="OQM83_004223"
     CDS             complement(<1..185)
                     /locus_tag="OQM83_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCS1522301.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALK"
     gene            complement(203..>493)
                     /locus_tag="OQM83_004224"
     CDS             complement(203..>493)
                     /locus_tag="OQM83_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1522302.1"
                     /translation="GATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAY
                     RDGLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
BASE COUNT          133 a          115 c          101 g          144 t
ORIGIN      
        1 tgcttcagtg ctgcatgatt cagtacatct cccataataa tcttgctatt agttggatag
       61 ggtttgtgga tttttgctgg ctgacgggca aataaagttt gtttaatggt ttgtttatct
      121 gccagttgat taatcacatg gcgggctatc tgaccgccag caccaagaat cagtacattt
      181 ttcataatat ttctcatgaa acttatccca gatgggtgtt gaaaaaagga ataacttttt
      241 ccagcgcctc ctgaactgct tccggtttgt catacagttc ataatgtgag aaatcgggta
      301 atgaaactaa ctgacgatcc tgcgaaacca tcgcccggcc atagacttcc agtccatcac
      361 gataagcacc gaaggcaccg actttttcac caactactac catcaccggc tgagtcagta
      421 acacttcagt aaatgcgaag gcatcccagg ccagcgtttt ctgtgcatgt gagaacaaca
      481 tttttgtcgc acc
//
LOCUS       DAJVQG010000409          491 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJVQG010000409 DAJVQG010000000
VERSION     DAJVQG010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.415"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>491
                     /locus_tag="OQM83_004225"
     CDS             <1..>491
                     /locus_tag="OQM83_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1522303.1"
                     /translation="KTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDG
                     SEIMKFVMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKS
                     FERKDESSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDST
                     ITD"
BASE COUNT          165 a           78 c          103 g          145 t
ORIGIN      
        1 ctaagacatt tttggaattt ttggtaaaaa tatttactct aggttatgtg gattttagca
       61 aacgctccaa cgaagcggga agaaatatga tggctcatat tgagtcctca tcttatatca
      121 aaaataatga tggcagtgag ataatgaagt ttgttatgaa taatcctgaa ggggaacgag
      181 cggatttatc taaggtggaa atagaaatca cactttccgc tttcactact atggggactc
      241 gtcaaggaca tacggctatt atatttcaac aacctgatgg ttcgactaat cgttatgaag
      301 gaaagtcctt tgaaagaaaa gatgagagtt cattacacct gattactaac aagattctgg
      361 catgttacca acgcgaagct aacaaggaaa tagctcgtct attaaatatt ccgcaggagt
      421 taaataattc tcaggacttg aataactcgc aggtgagttg taaagattca gttgattcta
      481 cgattacgga t
//
LOCUS       DAJVQG010000410          489 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.416, whole genome shotgun sequence.
ACCESSION   DAJVQG010000410 DAJVQG010000000
VERSION     DAJVQG010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.416"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            271..>489
                     /locus_tag="OQM83_004226"
     CDS             271..>489
                     /locus_tag="OQM83_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522304.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNAKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYISDQYDLVQHQLQQINAC"
BASE COUNT          187 a           90 c           70 g          142 t
ORIGIN      
        1 aaaaaaatca cttcacacac tatcagagaa cataataact ttttaaaatg agcgcatttt
       61 gatagttgtt aacgatttcc tttacgatac tatcactacc ctttttttat acacaaatat
      121 taccgggtgg agataaaagg gaaatcaaag gtaattatat caggaagata taacgcatta
      181 catttattgt gtgtagaaga aaatccattc ctgttctaat tatccaattt aaacatctta
      241 gcataaaaca aatgatgaat aaggaatttt atgtcaattg actttacccc aggtataata
      301 aatacatatc acggcgatat ttataactgc acaacaaata ccgataatgc taaaacgcca
      361 gacacaccga agtggccttg cgataattgg gaagaacaac aacccattaa ttccacattc
      421 tctggagaag gatatatctc tgatcaatat gatttagtgc aacaccaact tcaacaaatt
      481 aatgcgtgc
//
LOCUS       DAJVQG010000411          486 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.417, whole genome shotgun sequence.
ACCESSION   DAJVQG010000411 DAJVQG010000000
VERSION     DAJVQG010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.417"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>486
                     /locus_tag="OQM83_004227"
     CDS             <1..>486
                     /locus_tag="OQM83_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCS1522305.1"
                     /translation="DAEVYARVQAAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIK
                     RCPRKEAVAARGFVRWDGVEGTPVLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVA
                     DEPGSAGNTDDGIAMQLGTPVSGLPSTGYADTITDGEDIENLEIWRARVMERYYYIPQ
                     GG"
BASE COUNT          112 a          116 c          159 g           99 t
ORIGIN      
        1 ggatgcggaa gtgtatgccc gtgtacaggc tgcagccgta cacacgttgt acgggtatat
       61 cgattatctt gccagaaatc tgttaccgga cgtgtgtgat gaagactggc tgtaccggca
      121 cgccagaatc aaacgctgcc cgcgaaaaga agcggtggca gcccggggat ttgtgcgctg
      181 ggatggcgta gaggggacgc cggtattgcc agcgggaacg cagatccagc gtgataatca
      241 ggtgaccttt accacgacgg cgacggtgac cgcagccgat ggtcttctcc gggtgcctgt
      301 tgtggcagac gaaccgggat cggcggggaa tacggatgat ggtattgcca tgcaactggg
      361 aacacccgtc agtggtctgc cgtccacagg gtacgctgac accattacag acggtgaaga
      421 tattgaaaat ctggaaatat ggcgtgcccg cgttatggaa cgttattact acattccaca
      481 gggggg
//
LOCUS       DAJVQG010000412          485 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.418, whole genome shotgun sequence.
ACCESSION   DAJVQG010000412 DAJVQG010000000
VERSION     DAJVQG010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.418"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..190
                     /gene="bdm"
                     /locus_tag="OQM83_004228"
     CDS             <1..190
                     /gene="bdm"
                     /locus_tag="OQM83_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HCS1522306.1"
                     /translation="STAEPALVNAIEQGLRAEQGVVTEDDILMELTKWVEASDNDILS
                     DIYQQTINYVVSGQHPTL"
     gene            292..429
                     /gene="sra"
                     /locus_tag="OQM83_004229"
     CDS             292..429
                     /gene="sra"
                     /locus_tag="OQM83_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HCS1522307.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
BASE COUNT          145 a          113 c          110 g          117 t
ORIGIN      
        1 ttcaacagca gaacccgctc tggttaatgc cattgagcaa ggccttcggg cagagcaagg
       61 tgttgtcact gaagatgaca ttctcatgga gctgaccaaa tgggtggaag cttctgataa
      121 cgacatcctc agtgatatct accagcaaac gatcaattat gtggtcagtg gccagcaccc
      181 tacgctttaa ggtgctatgc ttgatcggca acctaattta ggggtttagc acgtgtttct
      241 tcgctacggc gatgttgtcc ttaaaactag caacaggatt gaggagttaa aatgaaatcg
      301 aaccgtcagg cacgtcatat tcttggactg gaccataaaa tttctaacca gcgcaaaata
      361 gttaccgaag gtgacaaatc cagcgtggta aataacccaa ccggcagaaa acgccccgct
      421 gaaaagtaat tcataaccat cagtcctcaa tgacgattaa acaccattgc ctgcgcaatg
      481 gtgtt
//
LOCUS       DAJVQG010000413          482 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.419, whole genome shotgun sequence.
ACCESSION   DAJVQG010000413 DAJVQG010000000
VERSION     DAJVQG010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.419"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..173
                     /locus_tag="OQM83_004230"
     CDS             <1..173
                     /locus_tag="OQM83_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522308.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            186..>482
                     /locus_tag="OQM83_004231"
     CDS             186..>482
                     /locus_tag="OQM83_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCS1522309.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGN"
BASE COUNT          120 a           92 c          139 g          131 t
ORIGIN      
        1 aggcgaaagc cttaatggcg caatatcggg taccggtagt cgtggaagtt attcttgagc
       61 gtgtgaccaa tatttcgatg ggcagcgaac tggataacgt catggaattt gaagatatcg
      121 ccgataacgc agtggacgca ccgactgaaa cctgcttcat gcactatgaa taagggagat
      181 aaataatgtt acgtttctct gctaatttat cgatgttatt tggtgaatat gattttctcg
      241 cccgttttga gaaagctgcg cagtgtggtt ttcgcggcgt tgaatttatg tttccttatg
      301 actacgacat tgaagaatta aaacaggtgc tggcgagtaa taaactcgaa catacgctgc
      361 acaatctacc ggcgggtgac tgggcggcgg gggagcgcgg tattgcctgt attcctggcc
      421 gtgaagaaga gtttcgggat ggcgtagcag cagcgattcg ttatgcccgt gcgctgggga
      481 at
//
LOCUS       DAJVQG010000414          480 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.420, whole genome shotgun sequence.
ACCESSION   DAJVQG010000414 DAJVQG010000000
VERSION     DAJVQG010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.420"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>480
                     /gene="hlyE"
                     /locus_tag="OQM83_004232"
     CDS             <1..>480
                     /gene="hlyE"
                     /locus_tag="OQM83_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HCS1522310.1"
                     /translation="IKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     "
BASE COUNT          156 a          100 c          106 g          118 t
ORIGIN      
        1 attaaggtac tggatgacgg catcacgaag ctgaatgaag cgcaaaaatc cctgctggta
       61 agctcacaaa gtttcaacaa cgcttccggg aaactgctgg cgttagatag ccagttaacc
      121 aatgattttt cagaaaaaag cagctatttc cagtcacagg tagataaaat caggaaggaa
      181 gcgtatgccg gtgccgcagc cggtgtcgtc gccggtccat ttggtttaat catttcctat
      241 tctattgctg cgggcgtagt tgaagggaaa ctgatcccag aattgaagaa caagttaaaa
      301 tctgtgcaga gtttctttac caccctgtct aacacggtta aacaagcgaa taaagatatc
      361 gatgccgcca aattgaaatt aaccaccgaa atagccgcca tcggagagat aaaaacggaa
      421 actgaaacca ccagattcta cgttgattat gatgatttaa tgctttcttt gctaaaagaa
//
LOCUS       DAJVQG010000415          478 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.421, whole genome shotgun sequence.
ACCESSION   DAJVQG010000415 DAJVQG010000000
VERSION     DAJVQG010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.421"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..109)
                     /locus_tag="OQM83_004233"
     CDS             complement(<1..109)
                     /locus_tag="OQM83_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="camphor resistance protein CrcB; may be involved in
                     chromosome condensation; overexpression in Escherichia
                     coli protects against decondensation by camphor;
                     overexpressing the protein results in an increase in
                     supercoiling; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCS1522311.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLG"
     gene            202..>478
                     /locus_tag="OQM83_004234"
     CDS             202..>478
                     /locus_tag="OQM83_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1522312.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRA"
BASE COUNT          124 a          113 c          126 g          115 t
ORIGIN      
        1 tccccaacgg aatcgcctga tgcagcgggt taaatcgcat acttaacagc catctcgcca
       61 cgcttcccgt accaccgcca ataaaaactg ctaaaagaag ttgtaacact gcaaaatcct
      121 gctatttgat ttgtatgagt gataagtgta acgccgaata atcgtcgttg gcgaatttta
      181 cgactctgac aggaggtggc aatgctggtt gccgcaggac agtttgctgt tacatctgtg
      241 tgggaaaaga acgctgagat ttgtgcctcg ttgatggcgc aggcggcgga aaacgacgta
      301 tcgctgttcg tcctgccgga agcattgctg gcgcgcgatg atcatgatgc agatctatcg
      361 gttaaatcag cacagctgct ggaaggcgaa ttcctcggac gtttacggcg agaaagtaaa
      421 cgtaacatga tgacgacaat tctgacgatt catgttcctt caacgccggg gcgcgcat
//
LOCUS       DAJVQG010000416          476 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.422, whole genome shotgun sequence.
ACCESSION   DAJVQG010000416 DAJVQG010000000
VERSION     DAJVQG010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.422"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>476
                     /locus_tag="OQM83_004235"
     rRNA            <1..>476
                     /locus_tag="OQM83_004235"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAJVQG010000417          475 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.423, whole genome shotgun sequence.
ACCESSION   DAJVQG010000417 DAJVQG010000000
VERSION     DAJVQG010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.423"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..>475)
                     /locus_tag="OQM83_004236"
     CDS             complement(<1..>475)
                     /locus_tag="OQM83_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCS1522313.1"
                     /translation="TRTEVSTLCISQFRYSAQIRPSSVVTKDYTFKRPGWPGRFDQEG
                     QYQDYQRTQYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIV
                     LTGHPQANLNREWQVVASDLHGEQPQAVPGRRGSGTTLENHFAVIPADRTWRPQPL"
BASE COUNT           78 a          150 c          137 g          110 t
ORIGIN      
        1 caacggctgt ggccgccatg ttctgtctgc cgggattacc gcaaaatggt tctccagcgt
       61 ggtgcctgaa cccctgcgtc ccggtaccgc ctgcggctgt tcgccgtgaa gatcgctcgc
      121 caccacctgc cattcccggt tcaggttcgc ctgcggatgc cccgtcagca caattcgccg
      181 tcccggccat atctccggcg aacggcttgt tccgcgcgcc acttctgcgt tgtttcgcca
      241 gccgtccatc tgccagcggg caaagttctg cccgtgggca cccttgaaac gtccggggta
      301 gtcatacact tcatactgcg tgcgctggta atcctggtac tggccttcct gatcaaaacg
      361 ccctggccag ccgggacgtt taaaggtgta gtctttggtc actacggaag aaggacggat
      421 ttgtgcgctg tagcgaaact ggctgatgca gagggtactt acctcggtac gggtg
//
LOCUS       DAJVQG010000418          474 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.424, whole genome shotgun sequence.
ACCESSION   DAJVQG010000418 DAJVQG010000000
VERSION     DAJVQG010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.424"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..>474)
                     /gene="fimZ"
                     /locus_tag="OQM83_004237"
     CDS             complement(<1..>474)
                     /gene="fimZ"
                     /locus_tag="OQM83_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HCS1522314.1"
                     /translation="TIDYLRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLS
                     SKSECFYAGRAIQAGANGFVSKCNDQNDIFHPVQMILSGYTFFPSETLNYIKSNKCST
                     NSSTVTVLSNREVTILRYLVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIV"
BASE COUNT          147 a           87 c           88 g          152 t
ORIGIN      
        1 tacaattgaa tgcaaaccta gtttgccata aatattagat ttatgcgcac taactgtttt
       61 attgctaagt aataacttat cggcaatttc tttattagat aatccgctaa ccagataacg
      121 taatatggtc acttcacgat tagatagcac agtgaccgtt gaactattcg tactacattt
      181 attgcttttt atatagttga gcgtttcgct aggaaaaaac gtgtatccgg agaggatcat
      241 ctgaacggga tgaaaaatat cattctgatc attgcattta ctgacaaaac cgttagcacc
      301 agcttgtatc gctctgccag cataaaagca ttctgatttc gatgataaaa ataacacttt
      361 cactgtgctc tggatttgtt tgatcctttt caggaaggta aaaccgtctg ttccgggcaa
      421 gtctatatcc ataatgatta aatcaacagg acgggttcgg agataatcga tggt
//
LOCUS       DAJVQG010000419          473 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.425, whole genome shotgun sequence.
ACCESSION   DAJVQG010000419 DAJVQG010000000
VERSION     DAJVQG010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.425"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            1..473
                     /locus_tag="OQM83_004238"
                     /pseudo
     CDS             1..473
                     /locus_tag="OQM83_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
BASE COUNT          141 a           95 c           99 g          138 t
ORIGIN      
        1 ttaactatat tgcatcgcaa aagctctcac aaaacaagat taataaattt aataattacc
       61 ttttccgctg gcagggcctg gatggctcag aaattctgat gcataacttt cctgaagaca
      121 cttatgatag ccgagcacgt gctcgctctt tagaatatat tgaacaaaac tataatgaaa
      181 aagagatctg tccctatgcg cttatggtgt atggtgtatg gtgtcggtga tggcggcgct
      241 ggccccggtg aggaacatat agaacgatta acacgtatac gcaatattga tggcttacct
      301 catgttgatt tcagccgtgt tgataagttc ttcacttacg ccgacgcctt tcgcgaaagc
      361 ttgcccatta tttcaggtga actttatttc gaggctcatc aagggtgctt tacatcagag
      421 tcagcaacta aagctcataa tcgaaatatg gaaaataaat tacatgatgc tga
//
LOCUS       DAJVQG010000420          469 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.426, whole genome shotgun sequence.
ACCESSION   DAJVQG010000420 DAJVQG010000000
VERSION     DAJVQG010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.426"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>469
                     /locus_tag="OQM83_004239"
     rRNA            <1..>469
                     /locus_tag="OQM83_004239"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          130 a           99 c          144 g           96 t
ORIGIN      
        1 gggcccatcc tccaaggcta aatactcctg actgaccgat agtgaaccag taccgtgagg
       61 gaaaggcgaa aagaaccccg gcgaggggag tgaaaaagaa cctgaaaccg tgtacgtaca
      121 agcagtggga gcacgcttag gcgtgtgact gcgtaccttt tgtataatgg gtcagcgact
      181 tatattctgt agcaaggtta accgaatagg ggagccgaag ggaaaccgag tcttaactgg
      241 gcgttaagtt gcagggtata gacccgaaac ccggtgatct agccatgggc aggttgaagg
      301 ttgggtaaca ctaactggag gaccgaaccg actaatgttg aaaaattagc ggatgacttg
      361 tggctggggg tgaaaggcca atcaaaccgg gagatagctg gttctccccg aaagctattt
      421 aggtagcgcc tcgtgaattc atctccgggg gtagagcact gtttcggca
//
LOCUS       DAJVQG010000421          469 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.427, whole genome shotgun sequence.
ACCESSION   DAJVQG010000421 DAJVQG010000000
VERSION     DAJVQG010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.427"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            1..469
                     /locus_tag="OQM83_004240"
                     /pseudo
     CDS             1..469
                     /locus_tag="OQM83_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT          165 a           99 c           77 g          128 t
ORIGIN      
        1 gtccggataa ttcagatgtt ctcctgttca aaaagctatt ctccccttcg aaattaatga
       61 caattatcgg tgatgaaatt cctcttatat ctgaaaaaca gtcgctttca aaggtgcttt
      121 taaatgatga gaataatgaa ctgagtgatg gtacaaactt ctgggataaa aatcgtcaat
      181 taaccacaga tgaaatagct tgctatcttc agaagatcgc cgctaatgca aaaaatactc
      241 aagtcaatta tcctactggt ctctacgtcc cctactccac cagaactcac ctggaagacg
      301 ctctcaatga aaatattaag agcgatccat catggccgaa tgaagtccag ttattcccca
      361 taaatactgg cggacactgg atattagttt cgctacagaa aatagtaaat aaaaaaataa
      421 taaactacaa ataaaatgcg tcatattcaa ctcattgcgt gcactaggc
//
LOCUS       DAJVQG010000422          468 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.428, whole genome shotgun sequence.
ACCESSION   DAJVQG010000422 DAJVQG010000000
VERSION     DAJVQG010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.428"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            44..>468
                     /locus_tag="OQM83_004241"
     CDS             44..>468
                     /locus_tag="OQM83_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005147412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCS1522315.1"
                     /translation="MNRAWTFRHWKGTGTVGVMVATNDPERPAPDESVINAVRDHILP
                     LAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNALMLRDGVPEGRMYLSR
                     ISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQA"
BASE COUNT          115 a          112 c          140 g          101 t
ORIGIN      
        1 cgcagaccct gattatgtta tctgggcgaa agaagttcag ggtattaacc gtgcatggac
       61 tttcagacac tggaaaggca ctggaacggt tggcgtgatg gtggcgacaa acgatccgga
      121 acgcccggcc ccggatgaaa gcgtgattaa cgcagtcagg gaccacatcc ttcctctggc
      181 acctgttgcc ggaagcggat tgtatgtatt cggtgccaca gaaaaagtca tcccgatgac
      241 gattgcatta tcgaaagaca caccgcagat caggactgca ataaaagcag agctgaatgc
      301 actgatgtta cgggatggtg tgccagaggg gcgtatgtat ctgtccagaa tcagtgaggc
      361 catcagtttg tctgctggtg aagtggccca ccggttaatc gtcccttcat cggatattga
      421 cctgggggag accgagcttc cggtgctcgg cgagatcacc tggcaggc
//
LOCUS       DAJVQG010000423          467 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.429, whole genome shotgun sequence.
ACCESSION   DAJVQG010000423 DAJVQG010000000
VERSION     DAJVQG010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.429"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..225
                     /locus_tag="OQM83_004242"
     CDS             <1..225
                     /locus_tag="OQM83_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1522316.1"
                     /translation="DSYDNAMAESINGLYKAEVIHRKSWKNRAEVELAILTWVDWYNN
                     RRLLERLGHTPPAEAEKAYYASIGNDDLAA"
     gene            267..467
                     /locus_tag="OQM83_004243"
                     /pseudo
     CDS             267..467
                     /locus_tag="OQM83_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          150 a          110 c          110 g           97 t
ORIGIN      
        1 gactcgtatg acaacgcgat ggcggagagc atcaatggtc tttacaaagc ggaggtaata
       61 caccgtaaga gctggaaaaa ccgtgcagaa gtggaactgg ccatactcac gtgggtggac
      121 tggtataaca atcgacgatt gctggaaagg ctgggccata ctcctccggc agaagcagaa
      181 aaagcttatt atgcttccat cggaaacgat gatctggcag cctgagttca cagataaaac
      241 actctccagg aaacccgggg cggttcaacc ttcacgataa aaaacttttc attaaaggta
      301 aagcggatca atatccggca cttcattctc tgatatctcc gacccgcatt aatctgaaag
      361 aaatagaaat tcactggcgt gaagtgctac gtctggcgac ctcaataaaa cagggaacag
      421 tgacagcatc cctgatgctg agaaaacttg ccagttaccc caaacaa
//
LOCUS       DAJVQG010000424          467 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.430, whole genome shotgun sequence.
ACCESSION   DAJVQG010000424 DAJVQG010000000
VERSION     DAJVQG010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.430"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..289)
                     /locus_tag="OQM83_004244"
     CDS             complement(<1..289)
                     /locus_tag="OQM83_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1522317.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNII"
     gene            complement(389..>467)
                     /locus_tag="OQM83_004245"
     CDS             complement(389..>467)
                     /locus_tag="OQM83_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1870 family protein"
                     /protein_id="HCS1522318.1"
                     /translation="GNFIDWKIYQSVAAELYAHDLERLC"
BASE COUNT          154 a          102 c           99 g          112 t
ORIGIN      
        1 taatgatgtt attggtctgc aggatgccaa aaaagaaggc catatagcag cacatcccga
       61 gcatgataaa agggcggcga ccaaagcgat cggataataa tcctgcaaaa agcaagacac
      121 ttaatcgacc aatgcccagc gatgagataa ctatcgagac acccgcggca ttagtctgcc
      181 aaagtgtctc cagcgaggcc atattcaggc tcatcaaaat gacgcccata ccatgcacca
      241 ggtaattaaa atacaaccca agtgcggtag ggtaataggg atttttcata ggttctactt
      301 agcaaaggtg ctttaaaaag tgtagcgatg ttgtgtcagt atccattcat tattgttgta
      361 acaacgacaa ttcctttctg tggaaaaatt aacaaagccg ctccagatcg tgtgcatata
      421 attcagcggc taccgattga tagatcttcc agtcaataaa attgcca
//
LOCUS       DAJVQG010000425          466 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.431, whole genome shotgun sequence.
ACCESSION   DAJVQG010000425 DAJVQG010000000
VERSION     DAJVQG010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.431"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>466
                     /locus_tag="OQM83_004246"
     CDS             <1..>466
                     /locus_tag="OQM83_004246"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCS1522319.1"
                     /translation="HGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSNFGIPASIVAKY
                     LDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRVKNMLPSLYR
                     EDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAFQKEL"
BASE COUNT          125 a          120 c          109 g          112 t
ORIGIN      
        1 cacggcttca aaaacatcga taacgagcac atgtatcttg acccgatcaa agtcaccctg
       61 ctgactccgg ggatggaaaa agacggcacc atgagcaact ttggtattcc ggccagcatc
      121 gtggcgaaat acctcgacga acatggcatc gttgttgaga aaaccggtcc gtataacctg
      181 ctgttcctgt tcagcatcgg tatcgataag accaaagcac tgagcctgct gcgtgctctg
      241 actgacttca aacgtgcgtt cgacctgaac ctgcgtgtga aaaacatgct gccgtctctg
      301 tatcgtgaag atcctgaatt ctatgaaaac atgcgtattc aggaactggc tcagaatatc
      361 cacaaactga ttgttcacca caatctgccg gatctgatgt atcgcgcatt tgaagtgctg
      421 ccgactatgg taatgactcc gtatgctgcg ttccagaaag agctgc
//
LOCUS       DAJVQG010000426          464 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.432, whole genome shotgun sequence.
ACCESSION   DAJVQG010000426 DAJVQG010000000
VERSION     DAJVQG010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.432"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(105..>464)
                     /locus_tag="OQM83_004247"
     CDS             complement(105..>464)
                     /locus_tag="OQM83_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HCS1522320.1"
                     /translation="TGRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLG
                     ERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTASGKIQKFLL
                     RKDIMRRLTQDVCEEIE"
BASE COUNT          127 a          111 c           93 g          133 t
ORIGIN      
        1 gacaccgtgg tgcaaacctg gttaagcttg gctgaactga agaaataaaa taaatccccg
       61 gcggcgttca gtcgccgggg ttatgtgatc cccgaagatg aaacttattc aatctcttca
      121 cagacatcct gagttaaccg ccgcataata tcttttctta acaaaaactt ttgtatttta
      181 cctgaggcag tacgcggtag tttttcgatt accacgatat gttcaggata tttatatttt
      241 gcgacccgtt tacggctaaa aaaagccacc acctcttcca gcgataatga atgatgcggc
      301 gctttcagca cgacataagc gcatgatcgt tcacctaaac gttcatcggg cattgcaacc
      361 acacaggcat cgtgaatttt aggatgctgc aataaaatat cttctacttc acggctgcta
      421 atattttcgc cgccgcggac aataatatct tttttgcgtc cggt
//
LOCUS       DAJVQG010000427          463 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.433, whole genome shotgun sequence.
ACCESSION   DAJVQG010000427 DAJVQG010000000
VERSION     DAJVQG010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.433"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>463
                     /locus_tag="OQM83_004248"
     CDS             <1..>463
                     /locus_tag="OQM83_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCS1522321.1"
                     /translation="YGNQTRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPDYHVEY
                     ARHWYSVPHELVGQRLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHMPERHR
                     RHGTWTPERLIEQGNRTGPSTGRVVESMLKAKPHPELAYRAVLGLLALQKKY"
BASE COUNT          115 a          139 c          124 g           85 t
ORIGIN      
        1 tacggtaatc aaacccgcgc agaacgcttc cgcatgctgg atgcaccagc actctctccg
       61 ctgcctctgg agccttatga atacacggaa tacaaagccg tgaaggttgg ccctgactac
      121 cacgttgaat atgcccgtca ctggtactcc gttccacacg aactggtcgg tcagcgtctg
      181 tcactgaagg tggggcagtc ggtggtacag ctctggcaca aggggcagtg tgtggcgcaa
      241 catccgcgca gcacacatga atataaacac acaaccaatc cgcttcacat gccagaacgc
      301 caccggcggc atgggacatg gacaccggaa cgccttatcg agcagggaaa ccgtacaggt
      361 ccgtccacag gccgggtggt tgaatccatg ctgaaagcca aaccccatcc ggagctggct
      421 taccgtgcag tcctgggtct gctggcactt cagaaaaaat acg
//
LOCUS       DAJVQG010000428          461 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.434, whole genome shotgun sequence.
ACCESSION   DAJVQG010000428 DAJVQG010000000
VERSION     DAJVQG010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.434"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>461
                     /locus_tag="OQM83_004249"
     CDS             <1..>461
                     /locus_tag="OQM83_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1522322.1"
                     /translation="LLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTL
                     RSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQ
                     GASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          134 a           94 c          143 g           90 t
ORIGIN      
        1 tctgctgacg tcgatgacgg acgccatgcg cttccccgga ggagaaatgg cggatctgta
       61 cagtcatcgc tgggaaatcg aactgggata cagggagata aaacagacga tgcaactgag
      121 caggctgacg ctgagaagta aaaagccgga gcttgtggag caagagctgt ggggtgtctt
      181 actggcttat aatctggtga gatatcagat gattaaaatg gcggaacatc tgaaaggtta
      241 ctggccgaat caactgagtt tctcagaatc atgcggaatg gtgatgagaa tgctgatgac
      301 attgcagggc gcttcaccgg gacgtatacc ggagctgatg cgcgatcttg caagtatggg
      361 acaacttgtg aaattaccga cgagaaggga aagggccttc ccgagagtgg taaaggagag
      421 gccctggaaa taccccacag ccccgaaaaa gagccagtca g
//
LOCUS       DAJVQG010000429          460 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.435, whole genome shotgun sequence.
ACCESSION   DAJVQG010000429 DAJVQG010000000
VERSION     DAJVQG010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.435"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(1..221)
                     /locus_tag="OQM83_004250"
                     /pseudo
     CDS             complement(1..221)
                     /locus_tag="OQM83_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          148 a           93 c           72 g          147 t
ORIGIN      
        1 atggtatcgt gaacaggaat accattttca aaatcaccat attgttttaa aaaatcgaga
       61 tgtgtttccc caaaatcctc tatatcttcc cagccttctg caccagaaat aacggcacaa
      121 atagtcaaca gtagaatatc cgataattta tgttccactt tccaggcttg tttttaatcg
      181 gggataatag aaatatgttc catcaatttt ttaagttcca ttttgttctc cttaattatg
      241 taaggagtat ttgatcatgt ataagcaata aaaaacagct tcaggtaata aggaatatct
      301 caatttttaa acataaaatg cgaattattt agtacaaaaa gtgaactcct cagggcaaga
      361 ttacgaaagt ccgctccccg caaggactga cgcgagatag tttctgtcca tggctgcttt
      421 tcgcatctta cgtcttaacc ctgccttgaa taccttatca
//
LOCUS       DAJVQG010000430          450 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.436, whole genome shotgun sequence.
ACCESSION   DAJVQG010000430 DAJVQG010000000
VERSION     DAJVQG010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.436"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..337)
                     /locus_tag="OQM83_004251"
     CDS             complement(<1..337)
                     /locus_tag="OQM83_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522323.1"
                     /translation="MRSVYEKIQSKLEDEIDEMVISMELQTNSYAYFSRLDRDGWGYD
                     PVEIGKVIADLPEGQRVVRTERAVRAPHRLSLIVADKSQAELEEIAKEKLMKARENEI
                     NLLKSRLAKL"
BASE COUNT          121 a          104 c           73 g          152 t
ORIGIN      
        1 tcagcttagc cagtcttgat ttcagcaaat taatctcatt ttctcttgct ttcatcagtt
       61 tctctttggc aatttcttca agctcagctt gtgatttatc agcaacaatt agagacagtc
      121 gatgtggtgc tctgacagct ctttctgtac gaacaactct ttgaccttct ggtaaatctg
      181 caatcacttt accaatctct acagggtcat acccccaacc gtcacggtct agtcgagaga
      241 aataagcata agaattggtt tgaagctcca tcgaaataac catttcgtca atttcgtctt
      301 caagcttgga ctgaattttc tcgtaaacac ttctcattgc ttcttgagag tctttaatag
      361 cttggaaata tcggcgttgt agcttcaact tcaaatcaag ttcacaccgt tcgttcatag
      421 aaaaatcaat ttcaactctt ggaatctcga
//
LOCUS       DAJVQG010000431          436 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.437, whole genome shotgun sequence.
ACCESSION   DAJVQG010000431 DAJVQG010000000
VERSION     DAJVQG010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.437"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>436
                     /locus_tag="OQM83_004252"
     CDS             <1..>436
                     /locus_tag="OQM83_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1522324.1"
                     /translation="TLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRH
                     WMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEV"
BASE COUNT          127 a          113 c          122 g           74 t
ORIGIN      
        1 accctgctgg ccatcgatgg tgtgttctgg cgcacaccgg atacaccaga gaacgatgca
       61 gccttccccc gccagacaca tgccgggaac ccggcgctct acccgcaggt caaaatggtc
      121 tgccagatgg aactgaccag ccatctgctg acggctgcag ccttcggcac gatgaagaac
      181 agcgaaaatg agcttgctga gcaacttata gaacaaaccg gcgataacac tctgacgtta
      241 atggataaag gttattactc actgggactg ttaaatgcct ggagcctggc gggagaacac
      301 cgccactgga tgatacctct cagaaaggga gcgcaatatg aagagctcag aaaactgggt
      361 aaaggcgatc atctggtgaa gctgaaaacc agcccgcagg cacgaaaaaa gtggccggga
      421 ctgggaaatg aagtga
//
LOCUS       DAJVQG010000432          434 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.438, whole genome shotgun sequence.
ACCESSION   DAJVQG010000432 DAJVQG010000000
VERSION     DAJVQG010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.438"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..108)
                     /locus_tag="OQM83_004253"
     CDS             complement(<1..108)
                     /locus_tag="OQM83_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCS1522325.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAF"
     gene            complement(120..>434)
                     /gene="rutB"
                     /locus_tag="OQM83_004254"
     CDS             complement(120..>434)
                     /gene="rutB"
                     /locus_tag="OQM83_004254"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCS1522326.1"
                     /translation="GDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTGIATNVCVEST
                     LRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSPTSFAR
                     IA"
BASE COUNT          114 a          119 c          123 g           78 t
ORIGIN      
        1 aaaagccagc gtaccggaga catacaccac gccatcagcc agcgtgccgg gaacgaaggg
       61 ggccagcggt gcgctgctgc cagcgggaat aattacggat tttggcatcg ttaaactcct
      121 taagcgatac gagcaaagga cgtgggagaa agcgcgtcgc agaacgtttc gacgtcgctg
      181 acccagccaa aaaaggtttc gatattgaac aacgcggctt tctgtgcaaa ttcaggcccc
      241 gcctggtgtg ttgcgtcttc cagcaccacg ccgaaatact ccagaaaaaa gccatcgcgt
      301 agcgtcgatt cgacgcagac gttggtagcg ataccggtga aaaccagatg gcgtattcca
      361 cggctgcgca aaatgctgtc cagcggcgta ttgaagaaac cgctgtagcg cggcttcggc
      421 agcacaatat cgcc
//
LOCUS       DAJVQG010000433          430 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.439, whole genome shotgun sequence.
ACCESSION   DAJVQG010000433 DAJVQG010000000
VERSION     DAJVQG010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.439"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..316)
                     /locus_tag="OQM83_004255"
     CDS             complement(<1..316)
                     /locus_tag="OQM83_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCS1522327.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVI"
BASE COUNT          109 a          115 c          100 g          106 t
ORIGIN      
        1 taatcacccc cagttgcatc cagagggttt tcgcgccaat ggcaatagct tcctgtgcca
       61 cgccccacgc cgcttctgaa ttacggaaaa catcgaccat atcgaccttt tccggaacgt
      121 ctgccagcgt gccatacccc tgctggccca gcagcgtttt gccggcaact tttggcgaaa
      181 ccggaatgac gtgatacccc tggtcgagta gatatttcat cacgcgatag ctgggacgat
      241 cgggtttgtc gcttgccccc accagcgcaa tggtgtgagt ggacgttaaa atgcctgcaa
      301 tatcggtttc tttcattatt tcctccagac tgtttaacaa agtgtacgac aaagccgaca
      361 tgccaaccat gcagctaata cgaatgaatg agagcaaaag cgggaaatat gtctatatgt
      421 tagtaagtat
//
LOCUS       DAJVQG010000434          428 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.440, whole genome shotgun sequence.
ACCESSION   DAJVQG010000434 DAJVQG010000000
VERSION     DAJVQG010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.440"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            111..320
                     /gene="asr"
                     /locus_tag="OQM83_004256"
     CDS             111..320
                     /gene="asr"
                     /locus_tag="OQM83_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HCS1522328.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHAGKHSHQQPAKPAAQPAA"
BASE COUNT          120 a          118 c          111 g           79 t
ORIGIN      
        1 gccattccca gggatatagt tatttcaacg gccccgcggt ggggttaaat gaaaaaacaa
       61 attgagggta tgacaatgaa aaagtattag ctctggttgt tgccgctgct atgggtctgt
      121 cttctgccgc ctttgctgca gagactgcga ccacacctgc tccgactgcg acgaccacca
      181 aagcagcgcc ggcgaaaacg acacatcata aaaaacagca taaagcagca cctgcccaga
      241 aagcgcaggc agcgaagaaa cacgccggga aacacagcca tcagcaaccg gcaaaacctg
      301 ctgcacaacc cgcagcgtaa gttttcaaca gtaatgctgg cgcgccccct cgcgcctgaa
      361 aattacggtg ctaagcgggt aacgtttagc accgccttta gcggagggta gtatggtggg
      421 ccgttatc
//
LOCUS       DAJVQG010000435          421 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.442, whole genome shotgun sequence.
ACCESSION   DAJVQG010000435 DAJVQG010000000
VERSION     DAJVQG010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.442"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            28..240
                     /locus_tag="OQM83_004257"
     CDS             28..240
                     /locus_tag="OQM83_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522329.1"
                     /translation="MSNTQKIINTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
     gene            273..>421
                     /locus_tag="OQM83_004258"
     CDS             273..>421
                     /locus_tag="OQM83_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCS1522330.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTE"
BASE COUNT          145 a           69 c           93 g          114 t
ORIGIN      
        1 atttaatcct taactggagg tatatctatg tcaaatacac agaaaattat taacactgaa
       61 aaatataacg agtgggtgaa aaaattctct gagcagattt ttaaaattac tggcgacgag
      121 aatgtggcaa aaaatgaatt agaaccgtgg acacctgaag gaaacgcacc aaattattgc
      181 tggtgggagg ttgatccggt tgatgctgca aatgaagcca tgagttacca caacgattaa
      241 tgtcgggagg ccgcccgaaa gggcggtaag aaatgactac attattcaga aaagaatatc
      301 cgcaaaaaag tagggcgaca gaatttttgt ttctcattct gtttatcgta ttgatgatac
      361 cgatatcccc tctaattttt gtctgggcaa tcgggaaaat aattgagcca gttactgaat
      421 t
//
LOCUS       DAJVQG010000436          421 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.443, whole genome shotgun sequence.
ACCESSION   DAJVQG010000436 DAJVQG010000000
VERSION     DAJVQG010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.443"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(50..193)
                     /locus_tag="OQM83_004259"
                     /pseudo
     CDS             complement(50..193)
                     /locus_tag="OQM83_004259"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(197..>421)
                     /locus_tag="OQM83_004260"
     CDS             complement(197..>421)
                     /locus_tag="OQM83_004260"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCS1522331.1"
                     /translation="TERPLASMVKSMLLDAVEQLNKDDKPLLHSDQGWQYQMPRWQRW
                     LSDNGITQSMSRRGNCLDNAAMESFFSTLK"
BASE COUNT          112 a           89 c           75 g          145 t
ORIGIN      
        1 cgtaggtgcc caccttttca aatgatctgc gccccaaatc ttggacatcc tatgaaaaat
       61 gtttaagtcg atattgaacc ggactcatgc catcgagttt ctctttaatg cgatggtgat
      121 tgtagtattc aatgtaatca attatatctt gctttagatg atgtaactta ttgtatttat
      181 ttaggtagta acattcttat tttaaagtac taaaaaagct ttccattgct gcattatcta
      241 agcagttacc acgtcgggac atgctttgtg ttatgccatt atcactcaac caacgttgcc
      301 aacgaggcat ctgatattgc cacccctgat cactatgcaa caagggctta tcgtctttat
      361 tgagttgttc taccgcatct aatagcatgc tctttaccat tgatgctaat gggcgctcag
      421 t
//
LOCUS       DAJVQG010000437          421 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.444, whole genome shotgun sequence.
ACCESSION   DAJVQG010000437 DAJVQG010000000
VERSION     DAJVQG010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.444"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>421
                     /locus_tag="OQM83_004261"
     CDS             <1..>421
                     /locus_tag="OQM83_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1522332.1"
                     /translation="IVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKA
                     PMADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHA
                     PRLNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVG"
BASE COUNT          117 a           89 c           97 g          118 t
ORIGIN      
        1 gtattgtccg ttccgatatt gcctatcaga tttatcgtga tttcgccgaa aacaaagggc
       61 tttttgtacc tggtgccaat gatattccgg tatatgataa ggacggaaaa cttgtgggaa
      121 gactgggtaa agccccaatg gccgatttca gcagtgtgag ctcaaatggc gttgctacgc
      181 ttgtatcgcc tcagtatatc gtcagcgtaa agcataacgg aggatatcgg agtgtgagct
      241 ttggtaatgg gaaaaataca tattcccttg ttgaccgtaa taaccaccct tctattgact
      301 tccatgctcc acgtctgaat aaactggtta cagaagttat tccctcagcg gtaacatcag
      361 aaggaaccaa agccaatgct tataaataca ctgaacgtta caccgctttt tatcgggtgg
      421 g
//
LOCUS       DAJVQG010000438          420 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.445, whole genome shotgun sequence.
ACCESSION   DAJVQG010000438 DAJVQG010000000
VERSION     DAJVQG010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.445"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            41..116
                     /locus_tag="OQM83_004262"
     tRNA            41..116
                     /locus_tag="OQM83_004262"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Ile,seq:cat)
BASE COUNT          117 a           79 c           98 g          126 t
ORIGIN      
        1 tttattgcgt ggtttataag tattgttatt gcacactcaa ggccctttag ctcagtggtt
       61 agagcaggcg actcataatc gcttggtcgc tggttcaagt ccagcaaggg ccaccaaatc
      121 attatttacg tatcaggaag agttaagggt acaaggggta ctcgatggcg gtctatattt
      181 catgactaac gccatttcaa ggaagaagtg cggtgggtgg tttcgaatga gggctggtgt
      241 tttggtacgt ggcacgccac tgcaatgtat ttatccatca gtactcattc aaaagtacct
      301 ggaaaaaatg cctgctatgc caagcgggca aattgcttac atgtggaatt aagtaattaa
      361 cgataaagcc agagtattat catttattat accccttcat ttaatcaggt taattactca
//
LOCUS       DAJVQG010000439          419 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.446, whole genome shotgun sequence.
ACCESSION   DAJVQG010000439 DAJVQG010000000
VERSION     DAJVQG010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.446"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>419
                     /locus_tag="OQM83_004263"
     CDS             <1..>419
                     /locus_tag="OQM83_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HCS1522333.1"
                     /translation="QFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNKSF
                     RVNPTPLNDSLKLMERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRISG
                     SRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKS"
BASE COUNT          155 a           72 c           81 g          111 t
ORIGIN      
        1 cacaatttaa agttgatgat tttctgattg agttaagttt taactctcag aaagttttaa
       61 aaggcttgga aaaagccgag aaacaaacga tgcaggtagc atcacgaatt gagaagcgat
      121 tgaataagtc gtttagagtt aatcctacgc ctttaaatga ttctctcaaa ctaatggaaa
      181 gaaatgtcga taaaactgtt tccaagattg agcagcgtct taaaaacact agagcgttta
      241 agattaaaac tgaaattgag gatactttaa agccacttag acaaccaaga cagccacgaa
      301 ttagcggaag cagagcaatc acagcagctt actcagcgaa tatgagcaag cttcgtgact
      361 ttaatcctat gcttgagaag tattttaaaa gccagtatta cagcttaagc agtaaatcc
//
LOCUS       DAJVQG010000440          416 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.447, whole genome shotgun sequence.
ACCESSION   DAJVQG010000440 DAJVQG010000000
VERSION     DAJVQG010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.447"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>416
                     /gene="gspA"
                     /locus_tag="OQM83_004264"
     CDS             <1..>416
                     /gene="gspA"
                     /locus_tag="OQM83_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HCS1522334.1"
                     /translation="IALLCRGDRRKLALAGETIRLLQQAEQTSVFTAKQWRMIYRILG
                     DNRPRKMQLAVVMSGTIIAITCGWLLLSSFTAPFPVPAWLVPVTPVVKQDMTKDIAHV
                     VMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLAC"
BASE COUNT           95 a           97 c          121 g          103 t
ORIGIN      
        1 gatagcttta ttatgtcggg gcgatcgcag aaagctggcg cttgccggcg aaacaattcg
       61 cttattacag caagccgagc agaccagcgt tttcactgca aaacaatggc gaatgattta
      121 ccgcatattg ggtgataacc gcccccggaa aatgcagctt gccgtggtaa tgagtggaac
      181 cattatcgct attacctgtg gttggctact gctgtcctct ttcacagctc cgttccccgt
      241 tcctgcgtgg ctggtacccg taacgccagt agttaaacaa gatatgacta aggatattgc
      301 tcatgtggtg atgcgcgata gtgaggcgct aagcgtgtta tacggcgtat gggggtacga
      361 ggtcccagct gattcggcct ggtgcgatca ggcagtaaga gcggggctgg catgta
//
LOCUS       DAJVQG010000441          416 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.448, whole genome shotgun sequence.
ACCESSION   DAJVQG010000441 DAJVQG010000000
VERSION     DAJVQG010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.448"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..339
                     /locus_tag="OQM83_004265"
     CDS             <1..339
                     /locus_tag="OQM83_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCS1522335.1"
                     /translation="AHNGGIIGPGAVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEA
                     LVARTMIKHARNTLLVADHTKFAASAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENV
                     ELVVAEQEVS"
BASE COUNT          110 a           86 c          105 g          115 t
ORIGIN      
        1 gctcataacg gcgggattat cggcccagga gccgtggatt ttattgaagg cttccgcgca
       61 gattatttaa tcaccagtat aggtgccatt gaacatgacg gcaccctact ggaatttgat
      121 gtaaatgaag cgttagtcgc gagaacgatg attaaacatg cgcggaatac attgttagtg
      181 gccgatcata cgaagtttgc cgcgtctgct gccgtttcaa ttggcaatgc acggaatgtc
      241 agggcttttt ttactgatgc cccgcctccc aattctttct gccagttgtt aagtgaagag
      301 aatgttgaac tggtggttgc cgagcaagaa gtatcctgaa attgaaattt cggaaaatcc
      361 gctgggaatt tcagcggatt ttgttttcca taagcaaaaa aaacctgcca gcgatg
//
LOCUS       DAJVQG010000442          412 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.449, whole genome shotgun sequence.
ACCESSION   DAJVQG010000442 DAJVQG010000000
VERSION     DAJVQG010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.449"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>412
                     /locus_tag="OQM83_004266"
     CDS             <1..>412
                     /locus_tag="OQM83_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCS1522336.1"
                     /translation="SNNLSWFIYKYDELAELAAVIDRLYEFHQLTEQRPTNKPKNCQH
                     AVQVANASIRTPDNKIILENLNFHVSPGKWLLLKGYSGAGKTTLLKTLSHCWPWFKGD
                     ISSPADSWYVSQTPLIKSGLLKEIICKALPLPVDD"
BASE COUNT          122 a           89 c           85 g          116 t
ORIGIN      
        1 tcgaacaatt taagctggtt tatttataaa tatgacgaac ttgctgaact ggctgcggtt
       61 atcgatcgct tgtatgagtt ccatcaactc actgaacagc gccctacgaa taagcctaaa
      121 aattgccaac atgcggtaca agtggctaat gcgagtattc gtacgcctga taataagatc
      181 atattagaga acttgaactt tcatgtttcg ccaggcaaat ggctattact gaaaggctac
      241 tctggtgcgg gaaaaaccac actgcttaaa acattatccc actgctggcc gtggtttaaa
      301 ggtgatattt cttctcctgc tgacagttgg tatgtgtcac aaacaccgtt aatcaaaagc
      361 ggcttactga aagagattat ttgtaaagca cttcccctgc ccgtagacga ta
//
LOCUS       DAJVQG010000443          408 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.450, whole genome shotgun sequence.
ACCESSION   DAJVQG010000443 DAJVQG010000000
VERSION     DAJVQG010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.450"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            54..130
                     /locus_tag="OQM83_004267"
     tRNA            54..130
                     /locus_tag="OQM83_004267"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="OQM83_004268"
     tRNA            173..248
                     /locus_tag="OQM83_004268"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
BASE COUNT          115 a           86 c           97 g          110 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcg
//
LOCUS       DAJVQG010000444          405 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.451, whole genome shotgun sequence.
ACCESSION   DAJVQG010000444 DAJVQG010000000
VERSION     DAJVQG010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.451"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..>405)
                     /gene="dgcZ"
                     /locus_tag="OQM83_004269"
     CDS             complement(<1..>405)
                     /gene="dgcZ"
                     /locus_tag="OQM83_004269"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HCS1522337.1"
                     /translation="DHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLA"
BASE COUNT          131 a           94 c           82 g           98 t
ORIGIN      
        1 gctaagtaag ttgccagggt gcgtaatact acatcgccga ttaaatgccc gtaggtatca
       61 ttaaccaatt taaatcggtc aatatccaac aacattaaat aaagattcag aggatcagtg
      121 ttgcgtaact gatgatcaaa ggattcatca agaacccgac gccccggcaa tcccgtcaaa
      181 acatccatat tgctacggat cgtcagcaaa taaattttgt aatcggttaa tgccgcagta
      241 aaagaaagca acccctcctg aaaggcgtcg aaatgcgcgt cctgccagtg attttcaaca
      301 atagccagca ttaattcccg accacagtta tgcatatgtt gatgggcaga atccattagc
      361 cgaacgtaag gtaattcatc gttatcgagt ggccccagat gatca
//
LOCUS       DAJVQG010000445          404 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.452, whole genome shotgun sequence.
ACCESSION   DAJVQG010000445 DAJVQG010000000
VERSION     DAJVQG010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.452"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>404
                     /locus_tag="OQM83_004270"
     CDS             <1..>404
                     /locus_tag="OQM83_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCS1522338.1"
                     /translation="REEAFKKIHQQRDEFNKQNDSEAFAVKYDKEVYQPAIAACHKQS
                     EECYEVPIQQKRDFDINEQRRQTFLQSQKLSRKLQDDWVTLEKGQYPLTMKVSEINSK
                     KVAILMKIDDINQANERWKKDTEQLRRNGVIK"
BASE COUNT          153 a           72 c           95 g           84 t
ORIGIN      
        1 cgcgaagaag cctttaaaaa aattcaccaa cagcgtgatg agtttaataa acagaacgat
       61 agcgaggcgt tcgcggttaa atatgacaaa gaggtttatc aaccggcgat tgcggcatgt
      121 cataaacaga gtgaagagtg ttatgaagtg ccgattcagc agaaacgaga tttcgatatc
      181 aacgaacaac ggcgacagac ttttctgcaa tcacaaaaac tcagccgtaa attgcaggat
      241 gactgggtaa cgctcgaaaa agggcaatat ccgttaacga tgaaagtttc agaaataaac
      301 agtaaaaaag tcgcgatttt gatgaaaatt gacgatatca accaagctaa tgaacgctgg
      361 aaaaaagata ccgaacaact gcggcgcaat ggggtgatta agta
//
LOCUS       DAJVQG010000446          402 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.453, whole genome shotgun sequence.
ACCESSION   DAJVQG010000446 DAJVQG010000000
VERSION     DAJVQG010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.453"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>402
                     /locus_tag="OQM83_004271"
     CDS             <1..>402
                     /locus_tag="OQM83_004271"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCS1522339.1"
                     /translation="EQYIARVFNADRSYMVTNGTSTANKIVGMYSAPAGSTILIDRNC
                     HKSLTHLMMMSDVTPIYFRPTRNAYGILGGIPQSEFQHAAIAKRVKETPNATWPVHAV
                     ITNSTYDGLLYNTDFIKKTLDVKSIHFDSAW"
BASE COUNT          107 a          116 c           86 g           93 t
ORIGIN      
        1 cagaacagta tatcgctcgc gtctttaacg cagaccgcag ctacatggtg accaacggta
       61 cttccactgc gaacaaaatt gttggcatgt actctgctcc ggcaggcagc accattctga
      121 ttgaccgtaa ctgccacaaa tcgctgaccc acctgatgat gatgagcgat gttacgccaa
      181 tctatttccg cccgacccgt aacgcttacg gtattcttgg tggtatccca cagagtgaat
      241 tccagcacgc tgccattgct aagcgcgtga aagaaacacc aaacgcaacc tggccggtac
      301 atgctgtaat taccaactct acctatgatg gtctgctgta caacaccgac ttcatcaaga
      361 aaacactgga tgtgaaatcc atccactttg actccgcgtg gg
//
LOCUS       DAJVQG010000447          391 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.454, whole genome shotgun sequence.
ACCESSION   DAJVQG010000447 DAJVQG010000000
VERSION     DAJVQG010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.454"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..>391)
                     /locus_tag="OQM83_004272"
     CDS             complement(<1..>391)
                     /locus_tag="OQM83_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCS1522340.1"
                     /translation="MPADIRLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWL
                     VRGGVAKLDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVL
                     PAPLPPYRVLTGLVDRFGRTQTFHREAA"
BASE COUNT           71 a          134 c          116 g           70 t
ORIGIN      
        1 ggcggcttcg cggtggaacg tctgtgtgcg cccgaagcgg tccaccagcc cggtcagtac
       61 ccggtacggc ggcagcggcg caggcagcac ctcatccgct tccggcaccc gctcacacca
      121 gccgagcagc caccacggcc cctgcggact gtttgtcgcc agataacgat gcggacttaa
      181 gcggagttct tccggcagcg cctgccagag tgcggccagc cggtgacctt catccagttt
      241 cgccacgccg ccgcgcacca gccagagtga ttcgctgcgg ctgtaaccgt cctcaccggg
      301 aaacaggtgc tcaaaataca ggcttctgcc gccgttatca ctgagtatca gtgtgttatc
      361 gcgcagctgt aagcggatat ccgcaggcat t
//
LOCUS       DAJVQG010000448          391 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.455, whole genome shotgun sequence.
ACCESSION   DAJVQG010000448 DAJVQG010000000
VERSION     DAJVQG010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.455"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>391
                     /locus_tag="OQM83_004273"
     CDS             <1..>391
                     /locus_tag="OQM83_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1522341.1"
                     /translation="WSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYD
                     SRGQLIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRS
                     MEYDAAGRVIRLTSENGSHTTFRYDVLD"
BASE COUNT           93 a          112 c          118 g           68 t
ORIGIN      
        1 tggagccgtt acggtcagtt gctgagcttc accgactgtt ccggttatgt aacccgttat
       61 gaccatgacc gcttcgggca gatgacggcg gtgcaccgcg aggaagggct gagtcagtac
      121 cgcgcatacg acagccgtgg acagttaatt gccgtgaaag acacgcaggg ccatgaaacg
      181 cggtatgaat acaacgccgc cggtgacctg accaccgtca ttgccccgga cggcagcaga
      241 aacgggacac agtacgatgc gtggggaaaa gctatctgta ccacgcaggg cggtctgacg
      301 cgcagtatgg aatacgatgc tgccggacgg gtcatccgcc tgaccagtga aaacggcagc
      361 cacaccacct tccgttacga tgtactcgac c
//
LOCUS       DAJVQG010000449          389 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.456, whole genome shotgun sequence.
ACCESSION   DAJVQG010000449 DAJVQG010000000
VERSION     DAJVQG010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.456"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..353)
                     /locus_tag="OQM83_004274"
     CDS             complement(<1..353)
                     /locus_tag="OQM83_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105657.1"
                     /note="phosphorylates aminoimidazole ribotide (AIR) which
                     forms 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate
                     (HMP-P); synthesis of HMP-P from AIR is complex and
                     requires the product of thiC gene at least; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HCS1522342.1"
                     /translation="MPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDDPFGAL
                     MQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETTDLPCW
                     RHGEWLHLCSIALSA"
BASE COUNT          102 a          117 c           85 g           85 t
ORIGIN      
        1 tcggcagaca acgcaattga acagagatgt aaccattcgc catgtcgcca gcagggcaag
       61 tctgtcgtct ctaaaaaaag atcggcactg gggcggacca taaacgtaaa tgaacgttcc
      121 ccttgatcgt tcagatcgac aagcaccgtg gatgtccggt gccattcatc ttgcttcaga
      181 tacgtgatat cgactccctc agttagcagc gttctttgca ttaacgcccc aaaaggatca
      241 tcaccgaccc gacctataaa tccacttatt ccgcctaatc tggcgattcc caccgcaacg
      301 ttagctggcg cgccgccagg acaaggcagt aggcgcccgt ctgattctgg caagagatct
      361 acgaccgcat cccctaaaac ccatacttt
//
LOCUS       DAJVQG010000450          386 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.457, whole genome shotgun sequence.
ACCESSION   DAJVQG010000450 DAJVQG010000000
VERSION     DAJVQG010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.457"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>386
                     /locus_tag="OQM83_004275"
     CDS             <1..>386
                     /locus_tag="OQM83_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522343.1"
                     /translation="LYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAYFGLPEALMNR
                     FVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPARAAWIQFLIV
                     IPLMIIPMLGSNTVQDLMNTIINMTA"
BASE COUNT           81 a           98 c          102 g          105 t
ORIGIN      
        1 actgtattcc gtctcctcgg tgctgattaa cgtcttcgtc agcagcaaag agttgaaatt
       61 taccggcgga tcggtgcagg tattccacgg catggcggcg tattttggtc taccggaagc
      121 gttgatgaat cgctttgtcg gtctggtgtc ctttaccgcg atgttcggtt ccctgctgat
      181 gtggaccgca acgccggtga aaattttctt ctccgaaatc ccggaaggca tctttggtaa
      241 gaaaaccgtc gaactgaacg aaaacggcgt tccggcgcgc gcagcgtgga tccagttcct
      301 gatcgtcatc ccgctgatga ttatcccgat gctcggttcc aataccgtgc aggatctgat
      361 gaatactatt attaatatga ccgccg
//
LOCUS       DAJVQG010000451          384 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.458, whole genome shotgun sequence.
ACCESSION   DAJVQG010000451 DAJVQG010000000
VERSION     DAJVQG010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.458"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..>384)
                     /locus_tag="OQM83_004276"
     CDS             complement(<1..>384)
                     /locus_tag="OQM83_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522344.1"
                     /translation="RPRYVLIEGPADFNDRVDELFLAHQLPVAIYSYCQYQDGAAPGR
                     GAWTPFAEFSPEWQALQAARRIQAQTYFIDLPCWAQSEEVDDSPDTQEESQALLLRAT
                     RMDNSDTLWDHLFEDESQQTALPSAL"
BASE COUNT           88 a          106 c          103 g           87 t
ORIGIN      
        1 agtgcagagg gtaatgcagt ttgctggctt tcatcttcga acaagtgatc ccacagggtg
       61 tcgctgttat ccatgcgggt ggcacgcagc agcaaggcct ggctttcctc ttgcgtatca
      121 ggcgaatcgt ccacttcttc actctgcgcc cagcaaggca aatcgatgaa gtaagtttgt
      181 gcctgaatac gacgtgcggc ttgtagcgcc tgccactccg gcgaaaattc agcaaatggc
      241 gtccaggcac cacgcccggg ggctgcaccg tcctgatact ggcaataact gtaaatagct
      301 accggaagct ggtgggctaa aaacagttcg tctacccggt cattaaaatc agccgggcct
      361 tcaatcaata cgtatcgtgg ccgc
//
LOCUS       DAJVQG010000452          382 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.459, whole genome shotgun sequence.
ACCESSION   DAJVQG010000452 DAJVQG010000000
VERSION     DAJVQG010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.459"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..>382)
                     /locus_tag="OQM83_004277"
     CDS             complement(<1..>382)
                     /locus_tag="OQM83_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HCS1522345.1"
                     /translation="VINAIVKPFYERQMSKSDNPIDKVVRSKVEPTDQALTGDMNSLA
                     AVLWAMVQGKSKDVPQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTVL
                     DGKVIDERTTSHINRMQEDTLISSA"
BASE COUNT          102 a           78 c           71 g          131 t
ORIGIN      
        1 agctgagcta atcaaggtat cttcctgcat acgattaata tgcgatgtgg ttctttcatc
       61 aataaccttt ccatcaagta ccgtctgagt tgtgatattg attgtctgag gctgaatatt
      121 tactgacggt ttgataacct ttaaatcttg tgtgaatgtt tccactcgac ctttcagatt
      181 atttaatgct tcaggtgaaa actgtggaac atcttttgat ttgccctgaa ccatagccca
      241 taaaacagcg gctaaagagt tcatgtcacc agtcaatgct tggtctgtgg gttccacctt
      301 tgaccttaca actttatcaa ttggattatc tgacttactc atctgacgtt catagaacgg
      361 tttgacaatg gcattaatca ca
//
LOCUS       DAJVQG010000453          381 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.460, whole genome shotgun sequence.
ACCESSION   DAJVQG010000453 DAJVQG010000000
VERSION     DAJVQG010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.460"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..>381)
                     /locus_tag="OQM83_004278"
     CDS             complement(<1..>381)
                     /locus_tag="OQM83_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS1522346.1"
                     /translation="GPTLTFLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLA
                     SIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCC
                     STKAVVSINPIKLATKDALQQFKDV"
BASE COUNT          114 a           79 c           88 g          100 t
ORIGIN      
        1 acatctttaa actgttgcaa cgcatctttg gtggctagtt ttatgggatt aatacttact
       61 acagctttgg tactgcaaca ggtaactacg gggttctcaa tggctttagc cgctggacta
      121 caacaactca cttttatggg cgtatatgtc gttcccggcg aagtttttgc tggagcacaa
      181 caatttgctg atgagctttt actggcaatg atatgacgtt caaaccccag agaatccagg
      241 ataatactgg caagaacgga gacgccggct gcgataatcg cgtacaacaa ggtgactttc
      301 caaccaaagg tcatccacat tagtccgacg ataatgggat taagtaatgg tgaaacaaat
      361 aaaaaagtga gagtcggacc a
//
LOCUS       DAJVQG010000454          381 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.461, whole genome shotgun sequence.
ACCESSION   DAJVQG010000454 DAJVQG010000000
VERSION     DAJVQG010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.461"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            241..>381
                     /locus_tag="OQM83_004279"
     CDS             241..>381
                     /locus_tag="OQM83_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCS1522347.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSL"
BASE COUNT          116 a           67 c           66 g          132 t
ORIGIN      
        1 taaaacctta agattaacat tttaatataa cgagttgcat ttagttacat gtttaacact
       61 tgatttaaga tttgtaatgg ctagattgaa atcagatgta atccattagt tttatatttt
      121 acccatttag ggctgattta ttactacaca cagcagtgca acatctgtca gtacttctgg
      181 tgcttctatt ttagaggcag ctgtcaggtg tgcgatcaat aaaaaaagcg gggtttcatc
      241 atgtttaatg aagtccatag tattcatggt catacattat tgttgatcac taaaccttct
      301 ttgcaggcga cggctctctt gcagcacctt aaacaatcgc tggcaattac aggaaaatta
      361 cataatattc aacgttctct g
//
LOCUS       DAJVQG010000455          378 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.462, whole genome shotgun sequence.
ACCESSION   DAJVQG010000455 DAJVQG010000000
VERSION     DAJVQG010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.462"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
BASE COUNT          136 a           84 c           48 g          110 t
ORIGIN      
        1 cgccttatat ccctgccctg aaggacgggg ttttacggcg caccggataa gcctggagat
       61 tatctacacc agcatggaca tcattgtact atgccccgta catgccccat cacatcctca
      121 acatcaaagt attagagcct tgcaaaccat tatgcgtctc agtttgtctc atctatctta
      181 cgcaaaaaaa aatagaacta atatcaagcg gcgctatatt gcgtattaaa taaaaccacc
      241 tcaattacat taataaatct aaacaaacat catgcaaata atttccatgg ttatttctag
      301 gctatttaaa ttaaataaat aaattatgag tcaatcataa ataacaacct ttaatataat
      361 ttaactacca aatttata
//
LOCUS       DAJVQG010000456          377 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.463, whole genome shotgun sequence.
ACCESSION   DAJVQG010000456 DAJVQG010000000
VERSION     DAJVQG010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.463"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..332)
                     /locus_tag="OQM83_004280"
     CDS             complement(<1..332)
                     /locus_tag="OQM83_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCS1522348.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVR"
BASE COUNT          102 a           95 c          110 g           70 t
ORIGIN      
        1 cggacagcct cacttcccag gcgctggcgg gcctgaataa cggcactggg ggcaacgaag
       61 gggcgattgc ccggcagcat gatgtccagg cgattcacaa tctggtgaag aggttcttta
      121 cgctcaagcg ccatgccaac aatacaccag accatcattt cgaggggaag acggcgcttg
      181 cgtagcgtta cagtacctga ttcggcaagg caacgagaga tgagttcggg gtcgaggtaa
      241 tcccccagag aagtcagtgg gttacgcaga gaatcgtaac gggataccag atcaagagcc
      301 tgtccaatgt gcataaaaaa atccggaaac aagtgagcgt ttccggattc ttacacagcc
      361 actggatcgg tcaactg
//
LOCUS       DAJVQG010000457          374 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.464, whole genome shotgun sequence.
ACCESSION   DAJVQG010000457 DAJVQG010000000
VERSION     DAJVQG010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.464"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            53..283
                     /locus_tag="OQM83_004281"
     CDS             53..283
                     /locus_tag="OQM83_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HCS1522349.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
BASE COUNT          104 a           71 c          116 g           83 t
ORIGIN      
        1 ctcaagaaac gatcggcatc atagcggcaa aaggtaagaa aaaggtggca ggatgtttac
       61 gaaggcgtta tcggttgtct tattaacgtg tgcgctgttc tcaggacaac tcatggcagg
      121 gcacaaagga catgaatttg tgtgggtaaa gaatgtggat catcagctgc gtcatgaagc
      181 ggacagcgat gaattgcgtg ctgtggcgga agagtcggcg gaaggtttgc gcgagcattt
      241 ttactggcaa aaatcgcgca aaccagaagc gggacaacgt tgagtcgttt gatgccggat
      301 gcggcgtgac gccttacccg gcaggcaata tcatgcagat tcaatgagtt gtgagtaacc
      361 agaaaactgg cgtt
//
LOCUS       DAJVQG010000458          373 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.465, whole genome shotgun sequence.
ACCESSION   DAJVQG010000458 DAJVQG010000000
VERSION     DAJVQG010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.465"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..>373)
                     /locus_tag="OQM83_004282"
     CDS             complement(<1..>373)
                     /locus_tag="OQM83_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HCS1522350.1"
                     /translation="RTVPWMPSEREHLALSALLEALPEPVLSVDMKSKVDMANPASCQ
                     LFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLMEITPVYLQDE
                     NDQHVLTGAVVMLRSTIRMGRQ"
BASE COUNT           98 a          103 c           85 g           87 t
ORIGIN      
        1 actggcggcc catacgaatc gttgatcgca acatcaccac cgcaccggtc aggacgtgtt
       61 gatcattttc atcctgaaga taaacaggcg taatctccat caggaaattc tgcccattaa
      121 taacgacatg ctcgttatgc gaatcttgcg gttcgctttc cagccaacgt aaaaaattaa
      181 agccgttaat caattgtgcg gcggtatggt tgcgcaggcg atccaatttt tgcccaaaaa
      241 gctgacagct cgccgggttc gccatatcca ctttgctttt catatcgaca gagagcacag
      301 gttcaggcaa cgcctccagt aacgcgctca acgccagatg ctcacgttcg gaaggcatcc
      361 acgggacagt acg
//
LOCUS       DAJVQG010000459          372 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.466, whole genome shotgun sequence.
ACCESSION   DAJVQG010000459 DAJVQG010000000
VERSION     DAJVQG010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.466"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>372
                     /locus_tag="OQM83_004283"
     CDS             <1..>372
                     /locus_tag="OQM83_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCS1522351.1"
                     /translation="IRGGVPVCWPWFGPAAQQGLPAHGFARNLPWTLKSHNEDADGVA
                     LTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETTSALHTYFNVGDIAK
                     VSVSGLGDRFIDKVNDAKEDVL"
BASE COUNT           91 a           86 c          104 g           91 t
ORIGIN      
        1 attcgcggtg gcgtaccggt ttgctggccg tggtttggtc cggcggcaca acaaggtctg
       61 cctgcgcacg gttttgcccg caacctgccg tggacgctga aatcacataa tgaagatgct
      121 gatggcgtag cgctgacttt tgaattgacg caaagcgaag agacgaaaaa attctgtccg
      181 cacgacttta cgctgttagc gcatttccgc gtgggtaaaa cctgtgaaat cgatcttgaa
      241 tcacatggcg aatttgaaac cacctctgcc ctgcatacct actttaacgt gggtgatatc
      301 gctaaggtaa gcgtcagtgg gctgggcgat cgcttcattg ataaagtgaa tgacgcgaaa
      361 gaagatgtac tg
//
LOCUS       DAJVQG010000460          367 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.467, whole genome shotgun sequence.
ACCESSION   DAJVQG010000460 DAJVQG010000000
VERSION     DAJVQG010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.467"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(169..300)
                     /locus_tag="OQM83_004284"
                     /pseudo
     CDS             complement(169..300)
                     /locus_tag="OQM83_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
BASE COUNT          114 a           77 c           91 g           85 t
ORIGIN      
        1 tttcggtgaa tatattgtta attaacatca atcatcagag agacggcatc gggaaaggac
       61 aattctgtga agggcttcat aaaaacaagt gtaaaaggaa taaaaggcag aaataaaagt
      121 caggagtcat gcccgtttgc aaacaagtgg ttcacaaacg ggcaattttt aacgtaacga
      181 gcgactttcc atcgcctcga tgagcaccgc atcatgtttc agcgtttgcc atgcatggat
      241 gacttctgcc ggaaaatcaa cgtgaacgat aaaatctcgc ccgcggggac cgtagccatt
      301 gatcagttcg tcaatcggct gggtgaagcg atgcaggcgg ttaaatttgg taaccccgct
      361 gaacgca
//
LOCUS       DAJVQG010000461          366 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.468, whole genome shotgun sequence.
ACCESSION   DAJVQG010000461 DAJVQG010000000
VERSION     DAJVQG010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.468"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..127)
                     /locus_tag="OQM83_004285"
     CDS             complement(<1..127)
                     /locus_tag="OQM83_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCS1522352.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHC"
BASE COUNT          134 a           71 c           70 g           91 t
ORIGIN      
        1 ggcaatgctt aacgcaaaaa aaaccgcaaa cgcggctatg cgttgtgatg ttatccgaga
       61 cgccaatccc gacaaccgat tttgcggttg tgtgaggaat atagccccgg agaacaaaaa
      121 ctgtcaacgg gcaaaaaaat ttccgttatc aggaatgtat aaaattaaag cgatcgagca
      181 aaaaatcctt tctgcaaaac taaaaaagat ttcaataaaa tcaggcagtt aaaaacacgg
      241 aaaacggtat aaaaaagcgc ctcaaaatcc tgttttttct ttcagataca atgtcttatt
      301 gcatttttta aaacatgtca gtgtggtaca tggatatcga taccatcgcc aattataaac
      361 tgggcg
//
LOCUS       DAJVQG010000462          365 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.469, whole genome shotgun sequence.
ACCESSION   DAJVQG010000462 DAJVQG010000000
VERSION     DAJVQG010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.469"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            16..>365
                     /locus_tag="OQM83_004286"
     CDS             16..>365
                     /locus_tag="OQM83_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1522353.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTL"
BASE COUNT           87 a           85 c          117 g           76 t
ORIGIN      
        1 taaggctaaa aaatcatgac gataagtttt aatactgttc cgtcgaatac gctggtgccg
       61 ttgttctacg ccgagatgaa caattctgcg gcaaatacag cggtgaccag cgcgcctgca
      121 ttactgatcg ggcatgccag caacgatgca gccattgagg ttaacagcct ggtgctgatg
      181 ccatcggcag attatgcccg ccagatttgt ggggcgggga gccagctggc gcgtatggtg
      241 gaagtctacc gtcagacaga tcctttcggt gaactgtatg ttattgcagt accggaagcc
      301 agaggggcgg cggcgacggt cagggtgacg gttgccggag aagcagagga aagcggcacc
      361 ctgag
//
LOCUS       DAJVQG010000463          363 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.470, whole genome shotgun sequence.
ACCESSION   DAJVQG010000463 DAJVQG010000000
VERSION     DAJVQG010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.470"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            10..>363
                     /locus_tag="OQM83_004287"
     CDS             10..>363
                     /locus_tag="OQM83_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1522354.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGG"
BASE COUNT          119 a           79 c           94 g           71 t
ORIGIN      
        1 ggcctttgca tggatcttgc actggtaaca ggacagcgcg aaggagatct ggcggctatg
       61 agatgggagg atataagaga tggcaggcta tacgtggaac aacaaaaaac aggggcaaaa
      121 attcgcattt ctctccccac cacgatttcc agattgaatt taacacttgc cgatgtactg
      181 gataacttga aaaaaataaa cggtaaaaac gaaaagttac tcggagggaa gacagccagg
      241 acaatcgcag cacagttcag gattgcccga gacacatccg gattgaaatg ggaaggtgac
      301 ccaccgccat ttcatgaaat ccgaagttta tccggtcgcc tgcactcagc agaaaaaggc
      361 ggt
//
LOCUS       DAJVQG010000464          360 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.471, whole genome shotgun sequence.
ACCESSION   DAJVQG010000464 DAJVQG010000000
VERSION     DAJVQG010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.471"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>360
                     /locus_tag="OQM83_004288"
     CDS             <1..>360
                     /locus_tag="OQM83_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCS1522355.1"
                     /translation="SILRSEFDEIWKAMLTLQFHDILPGSCISRVYHETEKEYLKLEA
                     KTEKIISDAQSTLLSKIDTSSYKDPHILFNTTCFARNEWININNNWLKARVNSYGYAV
                     IVPKNKIVNGLKAESQSI"
BASE COUNT          122 a           65 c           63 g          110 t
ORIGIN      
        1 tcaattcttc gcagtgagtt tgatgaaata tggaaagcga tgctgacctt gcaattccat
       61 gacatccttc ccggctcatg tatttcaaga gtttatcatg aaactgaaaa ggaatatctt
      121 aaactagaag caaaaaccga gaaaattatt tctgatgcgc agtctacttt attaagtaaa
      181 attgacactt cttcttataa agatccgcac attttgttta ataccacctg ttttgctcgt
      241 aacgaatgga tcaatataaa caacaactgg ctcaaagcac gagtaaactc ttatggttat
      301 gctgtgattg ttccaaagaa taagatcgta aatggtctga aggcagaatc tcaaagtatt
//
LOCUS       DAJVQG010000465          358 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.472, whole genome shotgun sequence.
ACCESSION   DAJVQG010000465 DAJVQG010000000
VERSION     DAJVQG010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.472"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>358
                     /gene="fliD"
                     /locus_tag="OQM83_004289"
     CDS             <1..>358
                     /gene="fliD"
                     /locus_tag="OQM83_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCS1522356.1"
                     /translation="YKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAF
                     KTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKG
                     YLADDGIIDSAQDSINA"
BASE COUNT          128 a           72 c           87 g           71 t
ORIGIN      
        1 tataaagaag tagacccagg tgctgaagca caagataaaa ataatggtgc acttctcgga
       61 gacagcgttg tacgtacaat tcaaagcggc atccgcgcac aatttgcaaa cggtgccagc
      121 gacagtgcct tcaaaacatt aaacgagatc gggattaagc aggacggcac gacgggtaag
      181 ctgaaaattg atgatgataa actcaaaaaa gtcctgaatg aaaatacagc atccgtacgt
      241 gaattgctgg taggcgatgg taaagaaaca ggaattacca ccaaaattgc caccgaggta
      301 aaaggttacc ttgccgatga tggcattatt gatagcgcac aggacagtat taacgcca
//
LOCUS       DAJVQG010000466          353 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.473, whole genome shotgun sequence.
ACCESSION   DAJVQG010000466 DAJVQG010000000
VERSION     DAJVQG010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.473"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
BASE COUNT          109 a           69 c           76 g           99 t
ORIGIN      
        1 atgcatagta atgccgatct gttatgaatg gtggtgtcat caatactctg aggctggtca
       61 atcatcacag aaaaccagcc tttgaatcaa agcagtactc accggatacg atcatagtca
      121 catcgaacgc aattctggcg gtgccgcatt tttatttatt cattttctgt gagagaaggg
      181 caagctctat agagggaatg taaaaaagag agctccgaat ggagcactct ttaagaaatt
      241 ataacggaaa atcagaggtt tttataacat gtcgcataag caaacccaag cttttcaaat
      301 acgctttgat cacctgttgc cagggaaact acgtattgtt cgcttgagtt tgc
//
LOCUS       DAJVQG010000467          346 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.476, whole genome shotgun sequence.
ACCESSION   DAJVQG010000467 DAJVQG010000000
VERSION     DAJVQG010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.476"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>346
                     /locus_tag="OQM83_004290"
     CDS             <1..>346
                     /locus_tag="OQM83_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCS1522357.1"
                     /translation="TLNDFLIAPDEGDLKPDVVKRFEEMVAQAQQSAGAAAGNAQQTA
                     QDVAAAAGYARAAEQAKNDIDAALTGTLKTANHLSEIAAAGEKAQQKSRDNLGLKSAA
                     TMEAQSDIYDRTK"
BASE COUNT           96 a           91 c          110 g           49 t
ORIGIN      
        1 cacgctgaat gactttctga ttgctcctga tgagggcgac ctgaaaccgg atgtcgtcaa
       61 acgctttgag gaaatggtgg cgcaggcgca gcagagcgcc ggggccgcag ccggaaacgc
      121 acagcagacg gcgcaggatg tggcggcagc cgcaggttat gcccgcgcag cagagcaggc
      181 caaaaatgac attgatgctg cgctgaccgg cactctgaaa acggctaacc atctgtctga
      241 aatcgcagca gcaggcgaaa aggcacaaca gaagtcccgg gataatctgg ggctgaaaag
      301 tgcggccacg atggaagcac agagcgacat ttacgaccgg acaaaa
//
LOCUS       DAJVQG010000468          343 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.477, whole genome shotgun sequence.
ACCESSION   DAJVQG010000468 DAJVQG010000000
VERSION     DAJVQG010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.477"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
BASE COUNT           67 a          123 c          106 g           47 t
ORIGIN      
        1 acctgagaag aggaaacaga acggggactg gccggggaag ataaggaaga ggcgcgctgt
       61 ttacgggacg cttccgcccg ctgcgcctgc gtgctcagcg ccatgtgaaa acggcggccc
      121 gcgccgtccg tcacccccgt caccgcgccc gccacgtcac cctccgccgc ccggtggaaa
      181 gtcagtgagc gcccgaagcc atccaccacg accgtcagta cccggtatgc gggcggctcc
      241 ggccagctga gtatccacca cggcccctga aggctgtttg tcgccaggta cacatgcgga
      301 ctcaggcgaa catcttccgg cagcacctgc cagagcgcgc tta
//
LOCUS       DAJVQG010000469          342 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.478, whole genome shotgun sequence.
ACCESSION   DAJVQG010000469 DAJVQG010000000
VERSION     DAJVQG010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.478"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..>342)
                     /locus_tag="OQM83_004291"
     CDS             complement(<1..>342)
                     /locus_tag="OQM83_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522358.1"
                     /translation="QYIDSMGLASKYGHLNNGGYGAKPSKPPTPDPSKLPDIAKQLRL
                     PYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCMPQDDPQYPGMETKTASD
                     YLPQTNWSTTQ"
BASE COUNT           77 a           72 c           80 g          113 t
ORIGIN      
        1 attgagttgt tgaccaattt gtctgtggca ggtaatcact tgctgtcttt gtctccatcc
       61 ctggatactg gggatcatcc tgcggcatac aagtcagatt gggttttacc cacttcctgc
      121 aatacagtgt gtaatcgtaa gggcttaatg ctctgaagaa tgttttgaaa acattaggcg
      181 cactactggc ctggtcaata ggatatggca gtcttaattg tttcgctatg tctggcaact
      241 tacttggatc gggcgtaggc ggtttgctgg gtttcgctcc atatccgcca ttatttaagt
      301 gtccatattt agatgccagc cccattgaat ctatatactg aa
//
LOCUS       DAJVQG010000470          339 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.481, whole genome shotgun sequence.
ACCESSION   DAJVQG010000470 DAJVQG010000000
VERSION     DAJVQG010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.481"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>339
                     /locus_tag="OQM83_004292"
     CDS             <1..>339
                     /locus_tag="OQM83_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1522359.1"
                     /translation="IIAERAHQESLVKQKRIAQVWNHKTQLARELKKPMGKQAPGWLE
                     LSEDGSYYIVDEDKASLVNIIYDKRLSGMSMFAICKWLNEQGYPTINQRKVRISKTKK
                     PDGNWSALSV"
BASE COUNT          113 a           68 c           77 g           81 t
ORIGIN      
        1 tcatcatagc tgagagagca caccaagaat ctcttgttaa gcaaaagcgt atagctcagg
       61 tctggaacca taaaacacag cttgcaagag aacttaagaa gcctatgggt aagcaagccc
      121 ccggatggtt agagctatca gaagatggtt catactacat cgttgatgaa gataaagcgt
      181 cactggtgaa tatcatctat gacaagcgtc tttctggtat gtcgatgttt gctatctgta
      241 aatggctaaa cgaacaaggt tacccgacca ttaatcagcg taaggtcaga atatccaaga
      301 ctaagaaacc ggatggcaac tggtcagcat taagcgtta
//
LOCUS       DAJVQG010000471          337 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.482, whole genome shotgun sequence.
ACCESSION   DAJVQG010000471 DAJVQG010000000
VERSION     DAJVQG010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.482"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>337
                     /locus_tag="OQM83_004293"
     CDS             <1..>337
                     /locus_tag="OQM83_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1522360.1"
                     /translation="KECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPE
                     LPASLEYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTML
                     PELPTSLEVL"
BASE COUNT          102 a           90 c           50 g           95 t
ORIGIN      
        1 aaagaatgtc tcatcaatca gttcagtgag cttcaactga atcgtttaaa tctgtcctcg
       61 ctacctgaca acttaccacc tcaaatcact gttctggaaa ttactcagaa tgccctaata
      121 tcattaccag aattgccagc atcgctggaa taccttgacg cctgtgacaa tcacctgtca
      181 acacttcctg aattacccgc atctctgaaa catcttgatg tagataacaa ccaactaacc
      241 atgcttcctg aattgcctgc attgctggaa tatattaatg cagataacaa tcagctaacc
      301 atgcttcctg aattacctac atcgctggaa gtgctct
//
LOCUS       DAJVQG010000472          334 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.484, whole genome shotgun sequence.
ACCESSION   DAJVQG010000472 DAJVQG010000000
VERSION     DAJVQG010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.484"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>334
                     /gene="hyaA"
                     /locus_tag="OQM83_004294"
     CDS             <1..>334
                     /gene="hyaA"
                     /locus_tag="OQM83_004294"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCS1522361.1"
                     /translation="SSGRPFIEKLKRAAAGASAIIAWGTCASWGCVQAAWPNPTQATP
                     IDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVDRMGRPLMFYGQRIHDKCY
                     RRAHFDAGE"
BASE COUNT           73 a          103 c           92 g           66 t
ORIGIN      
        1 agcagcggtc gaccgtttat tgagaaactc aaacgtgccg ctgccggagc cagcgcgatt
       61 atcgcctggg gaacctgcgc gtcctggggc tgcgtgcagg ccgcgtggcc caatccgacg
      121 caggcaacgc ctatcgacaa agtcatcacc gacaaaccca ttatcaaagt acctggctgc
      181 ccgccgatcc cggatgtgat gagcgccatc attacttaca tggtgacctt tgatcgcttg
      241 ccagatgtcg acagaatggg ccgtccgctg atgttctatg gtcagcgaat ccacgataaa
      301 tgctatcgcc gcgcccactt cgacgccgga gagt
//
LOCUS       DAJVQG010000473          331 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.485, whole genome shotgun sequence.
ACCESSION   DAJVQG010000473 DAJVQG010000000
VERSION     DAJVQG010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.485"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..157)
                     /locus_tag="OQM83_004295"
     CDS             complement(<1..157)
                     /locus_tag="OQM83_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HCS1522362.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRI"
BASE COUNT           77 a           95 c           85 g           74 t
ORIGIN      
        1 gaatgcgggg tttgttctcc agcgcccagg caatctttgg tgccattccc gcgcctaatc
       61 ccagcgacgt ggcagccaga ctacaatatt tgagaaagct gcgccgggta acgccctgac
      121 gccgcatggc ctggtaaaat gtttcctcgt tattcatatc gcacgtctct tctccttgcg
      181 acaccggcag gacaccgggc gcttatggtc ggcaagcgac gtccctgtag caaggcaaac
      241 catcgcttca cgtgagatgc tgaaaacgaa agctcatcct tctgcactgg cgcacgtcgc
      301 cagaaagtat tgttaataaa gcgtagtgaa a
//
LOCUS       DAJVQG010000474          326 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.487, whole genome shotgun sequence.
ACCESSION   DAJVQG010000474 DAJVQG010000000
VERSION     DAJVQG010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.487"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..>326)
                     /locus_tag="OQM83_004296"
     CDS             complement(<1..>326)
                     /locus_tag="OQM83_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCS1522363.1"
                     /translation="TGEWIHADFKRVRNYAFHKRFFKLLQLGFDYWTPVGGAITPRER
                     KLVSGFVDYLCESVGREHTPALSEAAEQYLNTVATRRTRDTALLKSFEAFREWVTIQA
                     GFYTEH"
BASE COUNT           79 a           89 c           78 g           80 t
ORIGIN      
        1 aaatgttcgg tgtaaaatcc agcctgaatg gttacccact cgcgaaaagc ctcaaacgac
       61 tttagcaatg ccgtatcccg ggttctgcgt gtcgcaactg tattcagata ttgctctgcg
      121 gcttcgctca gagctggcgt atgttcccgg cctactgatt cacacaggta atcaacgaaa
      181 ccggacacca gttttcgttc gcgaggcgtg atcgccccac cgaccggagt ccagtaatcg
      241 aatcccagtt gcaggagttt gaaaaaacgc ttgtggaatg cgtagttacg cacacgctta
      301 aagtctgcgt gtatccactc acctgt
//
LOCUS       DAJVQG010000475          322 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.488, whole genome shotgun sequence.
ACCESSION   DAJVQG010000475 DAJVQG010000000
VERSION     DAJVQG010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.488"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            16..123
                     /locus_tag="OQM83_004297"
     CDS             16..123
                     /locus_tag="OQM83_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCS1522364.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           84 a           73 c           98 g           67 t
ORIGIN      
        1 aatacaggcg gctatatgac gctcgcagaa ctgggcatgg ccttctggca tgatttagcg
       61 gctccggtca ttgctggtat tcttgccagt atgatcgtga actggctgaa caagcggaag
      121 taacgtgcca tgcgggcgtc aggctgccgt aatggcaatt tgcgcccgga tcaggccgca
      181 ggggtgaaac tctgcggcct ttttcgtaag aaaaatgcaa cggttgcaag cggataatat
      241 tctgcggaat gcgcagcaca gagtgaagac gtgacggatt ccggaacaga aaaacagtcg
      301 tagaatcagc gtcaagccgg aa
//
LOCUS       DAJVQG010000476          322 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.489, whole genome shotgun sequence.
ACCESSION   DAJVQG010000476 DAJVQG010000000
VERSION     DAJVQG010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.489"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>322
                     /gene="pagP"
                     /locus_tag="OQM83_004298"
     CDS             <1..>322
                     /gene="pagP"
                     /locus_tag="OQM83_004298"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCS1522365.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTAR"
BASE COUNT           90 a           69 c           90 g           73 t
ORIGIN      
        1 acgtttagag aaaatattgc acaaacctgg caacagcctg aacattatga tttatatatt
       61 cctgccatca cctggcatgc acgtttcgct tacgacaaag aaaaaaccga tcgctataac
      121 gagcgaccgt ggggtggcgg ttttggtcag tcgcgatggg atgaaaaagg aaactggcat
      181 ggcctgtatg ccatggcatt taaggactcg tggaacaaat gggaaccgat tgccggatac
      241 ggatgggaaa gtacctggcg accgctggcg gatgaaaatt ttcatttagg tctgggattc
      301 accgctggcg taacggcacg cg
//
LOCUS       DAJVQG010000477          322 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.490, whole genome shotgun sequence.
ACCESSION   DAJVQG010000477 DAJVQG010000000
VERSION     DAJVQG010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.490"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>322
                     /locus_tag="OQM83_004299"
     CDS             <1..>322
                     /locus_tag="OQM83_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="activator of genes involved in phenylacetic acid
                     catabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HCS1522366.1"
                     /translation="CAERLDADLPMVQLSHRLLQESMNNPALSETESEAALQAMVCLL
                     RPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEWIAGETGMSVRSLYRMFADKG
                     LVVA"
BASE COUNT           84 a           70 c           90 g           78 t
ORIGIN      
        1 tctgcgcaga aagactggac gctgacttac ccatggtgca actcagtcat cgcctgttac
       61 aggagagcat gaataatccg gcactttctg aaacagaaag tgaagctgcg ctacaggcga
      121 tggtgtgtct gctgcgcccg gtacttcatc agcgggaatc tgttcaacct cgccgtgaac
      181 gtcagtttca aaaagtggtt acgttgatag acgataatat tcgcgaagag atattacgcc
      241 cggagtggat agccggagag acaggtatgt cagtacgtag tttgtaccga atgtttgccg
      301 ataaaggttt ggtagtcgcg ca
//
LOCUS       DAJVQG010000478          321 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.491, whole genome shotgun sequence.
ACCESSION   DAJVQG010000478 DAJVQG010000000
VERSION     DAJVQG010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.491"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..206)
                     /locus_tag="OQM83_004300"
     CDS             complement(<1..206)
                     /locus_tag="OQM83_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1522367.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLV"
BASE COUNT           85 a           84 c           63 g           89 t
ORIGIN      
        1 atgaccagta atgctttgcc cggttcagtc atgcccagca gtcgcttacc tcgtcgaaca
       61 aatatttcga tgccaagttc atcctccagt tcccgaatat gacggctgac gccggactgt
      121 gaggtaaaaa gcatattcgc aacctctgtc aggttgtaat cctgacgtgc agcctcgcgg
      181 attatcttta gttgttggaa attcacggta aactccgggc agttcagatt tcccgttatt
      241 gttaaagtct aatgcccggc ataacaaata ataaaaaccc gcatcttatt ccatcccgat
      301 ataacgctta gctcaccaat t
//
LOCUS       DAJVQG010000479          317 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.492, whole genome shotgun sequence.
ACCESSION   DAJVQG010000479 DAJVQG010000000
VERSION     DAJVQG010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.492"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>317
                     /locus_tag="OQM83_004301"
     CDS             <1..>317
                     /locus_tag="OQM83_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCS1522368.1"
                     /translation="FADEGLLNKVKERGTLLVGLEGTYPPFSFQGDDGKLTGFEVEFA
                     QQLAKHLGVEASLKPTKWDGMLASLDSKRIDVVINQVTISDERKKKYDFSTPYTISGI
                     QAL"
BASE COUNT           83 a           70 c           85 g           79 t
ORIGIN      
        1 ttttgcagat gaaggtctgc ttaataaagt caaagagcgc ggcacgctgc tggtagggct
       61 ggaaggaact tatccgccgt tcagttttca gggggatgac ggcaaattaa ccggttttga
      121 agtggaattt gcccaacagc tggcaaaaca tcttggcgtt gaggcgtcac taaaaccgac
      181 caaatgggac ggtatgctgg cgtcgctgga ctctaaacgc attgatgtgg tgattaacca
      241 ggtcaccatt tctgacgagc gcaagaaaaa atacgatttt tctacccctt acaccatttc
      301 cggtattcag gcgctgg
//
LOCUS       DAJVQG010000480          316 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.494, whole genome shotgun sequence.
ACCESSION   DAJVQG010000480 DAJVQG010000000
VERSION     DAJVQG010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.494"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>316
                     /locus_tag="OQM83_004302"
     CDS             <1..>316
                     /locus_tag="OQM83_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCS1522369.1"
                     /translation="ADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGGDFSLV
                     PDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLIVIA
                     DKA"
BASE COUNT           75 a           68 c           94 g           79 t
ORIGIN      
        1 tgcagatggt catttttgtg gcaatttgcc gaatacgacc catttccatg agcaaaccgt
       61 ggagttcccg atccagggag agatggttga tattgtggcg catggtgagt tgggcggcga
      121 cttttcgtta gtgcctgaca gctacgtgac gatggggccg aaaagcatca tggcggcgaa
      181 aaacttgctg ataattgtca gtggcgcagg taaagcgcag gcactgaaaa atgtgctgca
      241 aggtccggtt accgaagatg ttccagcttc cgtgttgcag ctgcatcctt cgctgatagt
      301 aatcgctgat aaagcc
//
LOCUS       DAJVQG010000481          314 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.495, whole genome shotgun sequence.
ACCESSION   DAJVQG010000481 DAJVQG010000000
VERSION     DAJVQG010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.495"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(252..>314)
                     /locus_tag="OQM83_004303"
     CDS             complement(252..>314)
                     /locus_tag="OQM83_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCS1522370.1"
                     /translation="QLFGNPQSPRLQQFLKGSLK"
BASE COUNT           92 a           71 c           71 g           80 t
ORIGIN      
        1 gtaaaaaaga tttaaatacc gcatcatgcg gcgtggaagt tgtcgatatt gtcatggcgt
       61 tccgtcaccc ctcaaattta agatgacgcg acaataacca cgattaacac ccaatgcagc
      121 aggcagtttt tatctttgga cagcgaattc agaagtattt ttagcggact gcaactttta
      181 catttcagcg gaagctgact cgcaaattcc ccatgaaaat actccggcag cgttagccac
      241 cggagaggaa attatttcag cgatccctta aggaactgtt gcagacgagg gctttgcggg
      301 ttgccaaata actg
//
LOCUS       DAJVQG010000482          310 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.497, whole genome shotgun sequence.
ACCESSION   DAJVQG010000482 DAJVQG010000000
VERSION     DAJVQG010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.497"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..289
                     /gene="yahK"
                     /locus_tag="OQM83_004304"
     CDS             <1..289
                     /gene="yahK"
                     /locus_tag="OQM83_004304"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HCS1522371.1"
                     /translation="TTLLKRDGTMTLVGAPATPHKSPEVFNLIMKRRAIADSMIGGIP
                     ETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRGDVKYRFVIDNRTLTD"
BASE COUNT           86 a           66 c           78 g           80 t
ORIGIN      
        1 taccaccttg ctgaagcgtg atggcaccat gacgctggtt ggtgcgcctg cgacaccgca
       61 taaatcgccg gaagttttca acctgatcat gaaacgccgt gcgatagccg attctatgat
      121 tggcggcatt ccagaaactc aggagatgct cgatttttgc gccgaacatg gcatcgtggc
      181 tgatatagag atgattcggg ccgatcaaat taatgaagcc tatgagcgaa tgctgcgtgg
      241 tgatgtgaaa tatcgttttg ttatcgataa tcgcacacta acagattgaa aaaattaata
      301 aatacgctgt
//
LOCUS       DAJVQG010000483          308 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.498, whole genome shotgun sequence.
ACCESSION   DAJVQG010000483 DAJVQG010000000
VERSION     DAJVQG010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.498"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>308
                     /locus_tag="OQM83_004305"
     CDS             <1..>308
                     /locus_tag="OQM83_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1522372.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     P"
BASE COUNT           67 a           82 c           92 g           67 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggaccc
//
LOCUS       DAJVQG010000484          307 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.499, whole genome shotgun sequence.
ACCESSION   DAJVQG010000484 DAJVQG010000000
VERSION     DAJVQG010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.499"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>307
                     /locus_tag="OQM83_004306"
     CDS             <1..>307
                     /locus_tag="OQM83_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCS1522373.1"
                     /translation="STSNKLIAFRMMRYAIAAMQNHLDAGYKTLPMVVLLLFYHGIES
                     PYPYSLCWLDCFADPKLARQLYASAFPLIDVTVMPDDEIMQHRRMALLELIQKHIRQR
                     "
BASE COUNT           74 a           78 c           69 g           86 t
ORIGIN      
        1 agcacctcaa acaaactgat cgcatttcgc atgatgcgtt acgctattgc cgcaatgcaa
       61 aatcaccttg atgctggata caaaacgttg ccgatggtgg tgctattgtt gttttaccac
      121 ggtattgaaa gcccctatcc ctattcgctg tgttggctgg attgtttcgc cgatcccaaa
      181 ctggcaaggc agctttatgc ctccgcattt ccgctgattg atgtcaccgt catgcctgat
      241 gatgaaatca tgcagcaccg acgtatggcg ctgctggagt taattcaaaa acatattcgt
      301 caacgcg
//
LOCUS       DAJVQG010000485          307 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.500, whole genome shotgun sequence.
ACCESSION   DAJVQG010000485 DAJVQG010000000
VERSION     DAJVQG010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.500"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..261)
                     /locus_tag="OQM83_004307"
     CDS             complement(<1..261)
                     /locus_tag="OQM83_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HCS1522374.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQLAVLIVPTTKDETIEQYATRVFDNWRLGD"
BASE COUNT           92 a           50 c           62 g          103 t
ORIGIN      
        1 atctcctaaa cgccaattgt caaaaactct tgtagcatat tgttcaattg tctcgtcttt
       61 ggttgtggga acaattaata cagcaagttg cacttgggta ttattgaaaa tagattctaa
      121 cttgtgcgtc agttctttct tttcttgtga gtttaagcta ctcgtaagat ccgtaacttg
      181 ttgctgtaat tgtgggacaa tagttggttg ggctgcaaca aaggaacaca cgaaagtgaa
      241 cattagtatg aaatagcgca ttataaattt ctcattaaat ctggataaat aaattgacgg
      301 cggcatc
//
LOCUS       DAJVQG010000486          305 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.501, whole genome shotgun sequence.
ACCESSION   DAJVQG010000486 DAJVQG010000000
VERSION     DAJVQG010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.501"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..158
                     /locus_tag="OQM83_004308"
     CDS             <1..158
                     /locus_tag="OQM83_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCS1522375.1"
                     /translation="TIPCKSDPDLCMQLDAWDAETSIPALLNGEHSVLYRTRYDQQSD
                     AWIMRLA"
BASE COUNT           67 a           80 c           67 g           91 t
ORIGIN      
        1 taaccattcc ttgtaaatct gaccccgatt tatgtatgca actcgatgcc tgggatgctg
       61 aaaccagtat ccctgccctg ctaaatggcg aacactctgt cctttaccgt acccgttacg
      121 atcaacaatc tgatgcctgg attatgcgtc ttgcctgatc caaaaagaac ccgtcgggat
      181 ggcgggttat ttgtcctggt tattcccccg ttgtaaaatc tctcctaaac ttaacggtac
      241 ggcaccacac ttcggggatg aaatgttcgc gctggtactt tttgtttgct acctggatgg
      301 cggtt
//
LOCUS       DAJVQG010000487          304 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.502, whole genome shotgun sequence.
ACCESSION   DAJVQG010000487 DAJVQG010000000
VERSION     DAJVQG010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.502"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            55..273
                     /gene="ygdR"
                     /locus_tag="OQM83_004309"
     CDS             55..273
                     /gene="ygdR"
                     /locus_tag="OQM83_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCS1522376.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT           90 a           54 c           80 g           80 t
ORIGIN      
        1 acgtcggttt ataaacagac tattatcata ggtgggccaa atgaggataa ccggatgaaa
       61 aaatgggcag taataatttc tgcagtcgga ctggcgtttg ctgtttccgg gtgttccagt
      121 gattacgtca tggcgaccaa agatggccgt atgattttga ccgatggaaa acctgaaatt
      181 gatgatgata ccgggctggt gagttatcac gatcagcaag gtaacgcgat gcaaattaac
      241 cgtgatgatg tttcgcaaat tattgaacgt taacaaataa ggtcagcatc cggctgtaat
      301 gccg
//
LOCUS       DAJVQG010000488          304 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.503, whole genome shotgun sequence.
ACCESSION   DAJVQG010000488 DAJVQG010000000
VERSION     DAJVQG010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.503"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..>304
                     /locus_tag="OQM83_004310"
     CDS             <1..>304
                     /locus_tag="OQM83_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HCS1522377.1"
                     /translation="QSKSLGESLGGLMSDMSHGVDSLTDISYRVRAFFDEVTLAYRSL
                     NDTQKAVADGLANGLLSALKGLAGIVAVRSGIGAVGGIWNLIRAISTLGNVANTAAG"
BASE COUNT           76 a           52 c           85 g           91 t
ORIGIN      
        1 tcaatctaaa agtcttggtg aatctcttgg tggcttgatg agcgatatgt cgcatggggt
       61 cgattcacta accgatatta gttacagggt acgagcgttc tttgatgagg tgacattagc
      121 ttatcgttca ctaaatgaca ctcaaaaagc tgtagctgat ggtcttgcaa atggcctgtt
      181 aagtgcatta aaggggcttg ctggtatagt cgctgtacgg tctgggattg gtgctgtagg
      241 tggtatctgg aacttaatta gggccatttc cacgcttgga aatgtggcaa atacagcagc
      301 agga
//
LOCUS       DAJVQG010000489          301 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.denovo.506, whole genome shotgun sequence.
ACCESSION   DAJVQG010000489 DAJVQG010000000
VERSION     DAJVQG010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.denovo.506"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            118..>301
                     /locus_tag="OQM83_004311"
     CDS             118..>301
                     /locus_tag="OQM83_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HCS1522378.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLG"
BASE COUNT           72 a           68 c           70 g           91 t
ORIGIN      
        1 tctgtttgac gacttcaaaa aagatccaaa acaagctgct ttgttagaga agttcgacaa
       61 cgctgctcaa taatattccg ggccagtctc tggccctgat tttccaagat gcttacaatg
      121 atatttaacc gcctgaaact tgcggtagac cgcgtcattg ccgcgttttc tgtcgccgtc
      181 atgcttgctc tggttgtatg cgtagtctgg caagtattta gccgctacgt acttaaccag
      241 ccaagtacat taaccgatga gctggcacga tttttgatga tctgggtagg gttattaggt
      301 g
//
LOCUS       DAJVQG010000490         5262 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.guided.202, whole genome shotgun sequence.
ACCESSION   DAJVQG010000490 DAJVQG010000000
VERSION     DAJVQG010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.guided.202"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..389)
                     /locus_tag="OQM83_004312"
     CDS             complement(<1..389)
                     /locus_tag="OQM83_004312"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="poxA; regulates pyruvate oxidase poxB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P lysine(34) lysyltransferase"
                     /protein_id="HCS1522379.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMN"
     gene            714..2522
                     /gene="frdA"
                     /locus_tag="OQM83_004313"
     CDS             714..2522
                     /gene="frdA"
                     /locus_tag="OQM83_004313"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCS1522380.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            2515..3249
                     /gene="frdB"
                     /locus_tag="OQM83_004314"
     CDS             2515..3249
                     /gene="frdB"
                     /locus_tag="OQM83_004314"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCS1522381.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            3260..3655
                     /gene="frdC"
                     /locus_tag="OQM83_004315"
     CDS             3260..3655
                     /gene="frdC"
                     /locus_tag="OQM83_004315"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCS1522382.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            3666..4025
                     /gene="frdD"
                     /locus_tag="OQM83_004316"
     CDS             3666..4025
                     /gene="frdD"
                     /locus_tag="OQM83_004316"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCS1522383.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            4088..5221
                     /locus_tag="OQM83_004317"
     CDS             4088..5221
                     /locus_tag="OQM83_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCS1522384.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
BASE COUNT         1298 a         1375 c         1403 g         1186 t
ORIGIN      
        1 tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat agtgaactca
       61 gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg gaataccggc
      121 ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat taaccagaga
      181 ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac caaatgaata
      241 tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc acgatcggca
      301 aagaaacgac ggatctccgc cataatcgcc gcacgtttta ataagttagg aatggatgcg
      361 ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga caagggcacg
      421 aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac agcggtgggc
      481 agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc ttcagtgata
      541 atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact tatccatcag
      601 actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg taactttcag
      661 gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat gtcgtgcaaa
      721 cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt gctgcaattg
      781 ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta tacccgatgc
      841 gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat catgacagct
      901 tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag caggatgtcg
      961 tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg tgggggtgcc
     1021 catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc atgaaaatcg
     1081 agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg ctgttccaga
     1141 cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg gatattctgg
     1201 ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc acgctggtgc
     1261 agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt tatcgttaca
     1321 acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc cacggcgttc
     1381 cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt tccggtatcc
     1441 tgatgaccga aggctgccgt ggtgaaggcg gtattctggt caacaaaaat ggctaccgtt
     1501 atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa aacaaatata
     1561 tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg cgtaaaggca
     1621 acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cttgcgtcac ctcggcgaga
     1681 aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac gttggcgtcg
     1741 atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg ggcggtatcg
     1801 aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt gaatgttcct
     1861 ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa ctggtggtct
     1921 tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt aatggcaacg
     1981 aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat ctggttaacc
     2041 aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca atggaagaag
     2101 gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag ctggcagagc
     2161 tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc aacaccgacc
     2221 tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg gcgcactccg
     2281 caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt tgcaccgagc
     2341 gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat ggcacgactc
     2401 gcctagagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc gtttacggtg
     2461 gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag gcgaatggct
     2521 gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga taccgcaccg
     2581 catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga tgcgctggtc
     2641 tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg tatggcgatt
     2701 tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa aaccttcctg
     2761 cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat tgaacgcgat
     2821 ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc gtacatcatc
     2881 ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca gatggcaaag
     2941 tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg cccgcagttt
     3001 ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg ttataacgaa
     3061 gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca gaacggcgta
     3121 tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt cgatccggct
     3181 gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc gaccctgaaa
     3241 ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc caatgacgtc
     3301 cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag gcacggcggt
     3361 tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga aaaatggccc
     3421 ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga tcattaacct
     3481 gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg caccgaaagc
     3541 ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca aaagtctctg
     3601 ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact ggtaaggagc
     3661 ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg ggcctcttcg
     3721 gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg gtgggtattc
     3781 tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt ctggcgttcg
     3841 cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg ctgtggtgtg
     3901 gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct gcgggcaaat
     3961 gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt atcgttacaa
     4021 tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg tatggaaacc
     4081 agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt gctccacatt
     4141 tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc ttatagagca
     4201 acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt attactttac
     4261 ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt tgtttgagtt
     4321 aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg ctcgagggga
     4381 aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttactgcta aacagtggaa
     4441 tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat tgcaggtgcc
     4501 ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc agcctgcatg
     4561 ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg gcgcactggc
     4621 tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct tccagccact
     4681 caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt acgcctgggg
     4741 atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg aagcttatgg
     4801 tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa ccccccttga
     4861 tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct actggcaaac
     4921 cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca atcctgacag
     4981 catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa aagcgattaa
     5041 gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga ccggcggatt
     5101 tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc tggctaacaa
     5161 aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg ctctacagta
     5221 aaatttcatc gggtccgaat tttcggacct tttctgcgct tt
//
LOCUS       DAJVQG010000491         4845 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.guided.211, whole genome shotgun sequence.
ACCESSION   DAJVQG010000491 DAJVQG010000000
VERSION     DAJVQG010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.guided.211"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            complement(<1..345)
                     /locus_tag="OQM83_004318"
     CDS             complement(<1..345)
                     /locus_tag="OQM83_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="catalyzes the hydrolysis of Xaa-His dipeptides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCS1522385.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGAD"
     gene            606..1064
                     /gene="gpt"
                     /locus_tag="OQM83_004319"
     CDS             606..1064
                     /gene="gpt"
                     /locus_tag="OQM83_004319"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCS1522386.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1156..2400
                     /gene="frsA"
                     /locus_tag="OQM83_004320"
     CDS             1156..2400
                     /gene="frsA"
                     /locus_tag="OQM83_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCS1522387.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            2458..2859
                     /gene="crl"
                     /locus_tag="OQM83_004321"
     CDS             2458..2859
                     /gene="crl"
                     /locus_tag="OQM83_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCS1522388.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(2898..3953)
                     /gene="phoE"
                     /locus_tag="OQM83_004322"
     CDS             complement(2898..3953)
                     /gene="phoE"
                     /locus_tag="OQM83_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCS1522389.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            4241..>4845
                     /gene="proB"
                     /locus_tag="OQM83_004323"
     CDS             4241..>4845
                     /gene="proB"
                     /locus_tag="OQM83_004323"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCS1522390.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIA"
BASE COUNT         1198 a         1173 c         1210 g         1264 t
ORIGIN      
        1 atctgcaccc agcgtggtgc cgcgcgcttt aacccattcg ccatcaatat aaggctggat
       61 aggatctttc gtgaagtcat gcacggtgtc gttatttttc tgcggcacca tatcgaggtg
      121 ggcctgtaag acgaccgatt tacgattttc catacctgcg gtagcaggtt tacgaatcag
      181 gatattacct acctgatcgc gttcgacatg gaaacctttc tcttttgccc aaccaacaat
      241 gtattcagcg agttgctctt catgatagga cgggtgagga atagaacaga ttttggcaaa
      301 aatatcccac agcggctgtg gagataattg agacagttca gacacgttaa gtctccttgt
      361 cgatcacccg caaaacagta ttgcaggtca cagggttagc agaaaatgtt gtcaacacaa
      421 gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg
      481 cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc
      541 tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac
      601 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac
      661 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg
      721 gtctggtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt
      781 gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctaaaa cgcgcagaag
      841 gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg
      901 cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc
      961 gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatt gaacagccgt
     1021 gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg
     1081 cactgccggg cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt
     1141 ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt
     1201 aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg
     1261 cagtcggccc ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg
     1321 tggatctggc gcggcattga cccacgtgaa atcctcgacg tccaggcgcg tattgtgatg
     1381 agcgatgccg aacgtaccga cgatgattta tacgatacgg tgattggcta ccgtggtggc
     1441 aactggattt atgagtgggc catccaggcg atggtgtggc aacaaaaagc ctgtgcggaa
     1501 gaagatccgc aactcagtgg tcgtcactgg ctgcatgcgg ctacgttgta caacattgct
     1561 gcctatcctc atctgaaagg agatgacctg gccgagcaag cgcaggcttt gtcaaaccgt
     1621 gcctatgaag aagccgccca gcgtctgccg ggcacgatgc ggcagatgga gtttaccgta
     1681 cccggcggtg cgcccatcac cggctttttg catatgccga aaggcgatgg cccgttcccg
     1741 acagtattaa tgtgtggtgg tctggatgcg atgcagacgg actattacag cctgtatgaa
     1801 cgttattttg cgccgcgcgg cattgcgatg ctgactattg atatgccgtc ggtgggcttt
     1861 tcatcaaaat ggaagctcac ccaggactcc agcctgttgc atcagcacgt tttaaaggct
     1921 ctgcctaacg taccgtgggt ggatcacact cgcgtcgcgg cctttggttt ccgtttcggc
     1981 gctaacgttg ccgtgcgtct ggcatatctt gaatcgccgc gtctgaaagc ggttgcctgt
     2041 cttggtccgg tagttcatac cctgttgagt gattttaagt gccagcaaca ggtgccggaa
     2101 atgtatcttg acgttctggc gagtcgtttg gggatgcatg atgcttccga cgaagcgtta
     2161 cgcgtggagc tgaatcgcta ttcattaaaa gtgcaaggat tgctggggcg tcgctgccca
     2221 acgccaatgt tatcaggcta ctggaagaac gatccgttca gcccggaaga ggactcacgc
     2281 ttaatcacct catcatctgc tgacggtaaa ttattagaga tcccatttaa cccggtgtat
     2341 cggaattttg acaaaggtct tcaggaaatc accggctgga tcgaaaaacg cttgtgttaa
     2401 aaatttgcta aaatttgcca atttggtaaa acagttgcat cacaacagga gattgcaatg
     2461 acgttaccga gtggacaccc gaagagcaga ttgatcaaaa aatttaccgc actaggcccg
     2521 tatattcgtg aaggtaagtg cgaagataat cgattctttt tcgattgtct ggctgtatgc
     2581 gtcaacgtga aaccggcacc ggaagtgcgt gagttctggg gctggtggat ggagcttgaa
     2641 gcgcaggaat cccgttttac ctacagttac cagtttggtc tgttcgataa agcaggcgac
     2701 tggaagagtg ttccggtaaa agacactgaa gtggttgaac gactggagca caccctacgt
     2761 gagtttcacg agaagctgcg tgaactgctg acgacgctga atctgaagct ggaaccggcg
     2821 gatgattttc gtgatgaacc ggtgaagtta acggcgtgag tgaaatgtgc cggatgtatc
     2881 acatccggca atatccatta aaactgatag gtcatgccaa ccgcgacaat atcatcatta
     2941 ttaatattca atttgttatc gctatccagt tggttgattt tataatcaac aaacgctgac
     3001 atatttttgt tgaaataata cgtagcaccg acgtcgatat aattgaccag atcttcatca
     3061 ccgatacctt caatatcttt ccctttcgat aagacataac ccagcgatgg acgcagacca
     3121 aagtcaaact ggtattgagc gaccgcttca aagttctgtg tcttattggc aaagccgcca
     3181 gttattggcg tcattttgcg tgtttcagaa tagaaagttg ccagataaat attattggca
     3241 tcgtatttca gacctgttgc ccatgcttct gcacgcttgc ctgtgccacg gctttgcagg
     3301 ttctgctcgt tggtgcgatc tgagttggta taggccccac taatggcgaa atcgctgccg
     3361 ccaaagtcat atgtcaatga cgtgccgaag ccatcgccgt tttgcttttt aacgtcgcgg
     3421 ttttcgtttt tcccttgata ttgcagggtt aagttcaggc catcgataac gccgaagaag
     3481 tcggtgttcc gatacgtcgc cagaccgctg gcgcgtttgg tcataaagtt gtcggtctgc
     3541 gccgaggaat cgccaccaaa ttccgggaac atatcggtcc aggcttccac gtcatacaac
     3601 gcgcccaggt tacgaccata atcgaaagaa cccaaatctt tatatttcaa cccggcaaaa
     3661 gcgagacgcg ttttctgctg tgcagtatca ctctccgctt tatttccggc aaactccgct
     3721 tcccaccgac cataaccagt cagttgatcg ttaatttgtg tttcaccttt aaaaccaaaa
     3781 cggatataac tctggtcgcc atctttactg gcgttatcac tcatataatg catagcttta
     3841 actttgccat agacatccag tttattaccg tctttattat atatttctgc agcctgtaca
     3901 gatgcagatg ccacaatgcc catcaccact aatgccagag tgctcttttt cattttcatt
     3961 cctgatttta attaacgcgc gaatattcag cgggagagcc ccgttgaaaa caggaaagtt
     4021 tttaacctga gattgttaaa gatatattac agattaataa tattcttaaa atgtggtaat
     4081 ttattaaatc tgtaataaaa gcgtaaacaa ctgccgctag gcttgctgat cccgcgcaac
     4141 aaaacgccat gctttgctcg cagatggttg gcaaccgacg acagtcctgc taaaacgttc
     4201 gtttgatatc atttttccta aaattgaatg gcagagaatc atgagtgaca gccagacgct
     4261 ggtggtaaaa ctcggcacca gtgtgctaac aggcggatcg cgccgtctga accgtgccca
     4321 tattgttgaa cttgttcgcc agtgcgcgca gttacatgcc gccgggcatc ggattgttat
     4381 tgtgacgtcg ggcgcgatcg ccgccggacg tgagcacctg ggttacccgg aactgccagc
     4441 gactatcgcc tcgaaacaac tgctggcagc ggtagggcag agtcgactga ttcaactgtg
     4501 ggaacagctg ttttcgattt atggcattca cgtcgggcaa atgctgctga cccgtgctga
     4561 tatggaagac cgtgaacgct tcctgaacgc ccgcgacacc ctgcgtgcgt tgctcgataa
     4621 caatatcgtt ccggtaatca atgagaacga tgctgtcgct acggcagaga ttaaagtcgg
     4681 cgataacgat aacctttctg cgctggcggc gattctggcg ggtgccgata aactgttgct
     4741 gctgaccgat caaaaaggtt tgtataccgc tgacccgcgc agcaatccgc aggcagaact
     4801 gattaaagat gtttacggca ttgatgacgc actgcgcgcg attgc
//
LOCUS       DAJVQG010000492         4776 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.guided.213, whole genome shotgun sequence.
ACCESSION   DAJVQG010000492 DAJVQG010000000
VERSION     DAJVQG010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.guided.213"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            561..800
                     /locus_tag="OQM83_004324"
     CDS             561..800
                     /locus_tag="OQM83_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCS1522391.1"
                     /translation="MTPFRYNSDLTSGSLQTREYRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(988..1965)
                     /gene="intI2"
                     /locus_tag="OQM83_004325"
                     /pseudo
     CDS             complement(988..1965)
                     /gene="intI2"
                     /locus_tag="OQM83_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2297..2770
                     /locus_tag="OQM83_004326"
     CDS             2297..2770
                     /locus_tag="OQM83_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCS1522392.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2865..3389
                     /gene="sat2"
                     /locus_tag="OQM83_004327"
     CDS             2865..3389
                     /gene="sat2"
                     /locus_tag="OQM83_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCS1522393.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3458..3955
                     /locus_tag="OQM83_004328"
     CDS             3458..3955
                     /locus_tag="OQM83_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522394.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            4016..4387
                     /locus_tag="OQM83_004329"
     CDS             4016..4387
                     /locus_tag="OQM83_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522395.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
BASE COUNT         1354 a         1047 c         1102 g         1273 t
ORIGIN      
        1 ggatcggtca actgatcctt aactgatcgg cattacaccg gtcggcagcc ctgtgggcgg
       61 acaaaatagt tgggaactgg gaggggtgga aatggagttt ttaaggatta tttagggaag
      121 agtgacaaaa tagatgggaa ctgggtgtag cgtcgtaagc taatacgaaa attaaaaatg
      181 acaaaatagt ttggaactag atttcactta tctggttaac taatctagct aacctgccgc
      241 tcagcttagt acgatcctat atccaactga tcataatcct ttcatcagta tcccaaatta
      301 aatccccctc ctagactgcc tcaccaacac tttacagtga tcccgtattt ccggacaggt
      361 gattgatttt tttcgcttgt acaggtgtta agccacagtt atcttgatgt ggacattatg
      421 cattgtaata gcccattaac atgcgctaag agcataaatt aatatacagt aacaagcatt
      481 acgaggtgct aatgtttgga aagcagcctc ttccaactac aataataagt taactatcct
      541 ccacccacaa ataattacaa atgacccctt tccgctataa ctccgatctc accagcggct
      601 cgctacaaac ccgagagtat cgcattatta ctggcttgtt actccaagag cttgatgaag
      661 ctgcctggga taaagccatg tataaggaaa atgtgcttca gaaacgcacg caatctacgg
      721 taagacgtat ttcttcagcg cttagaaaac gcctagaaca cctaagctct gacttttggg
      781 cttttgcgtt tttatgttag gcattgtctg gcaactggag gaagtgtaat gaccatgaat
      841 ggtcactgtg attgacagta tcttgcttaa acttatctga acaatggtag aactggctca
      901 tatttatccc ctctatgatt gtttatcttt ttcaccataa aaagtatcag gggctactca
      961 gggctacata aagatattta tctactttta ctgattgata agtagcatca gtccatccgc
     1021 aggactggtg gtgccggcaa aatgctgacc caacacatgc gtatagattt gcgtggtctt
     1081 aacatcgtta tgccctaaga gttcttgcac agtgcgaata tcacgccccg cttgtaatag
     1141 atgcgtagca aacgagtgac gaaatgtatg acaagtgaca cgcttgctaa cgatgcctgc
     1201 tttttgtacg gctgccttca atgcctttcg cgcaacggag tcatgcagat gatggcggca
     1261 taatttgccg ttatacgggt ggttgcagag cgtgctggag ggaaagacaa acatccacgc
     1321 cgcttgtcga taagcagaag ggtatttgtg atctaaagca aaaggcagcg atggccctac
     1381 gccttgtaag ttgtcgtctt gctgaataag ccgcgcttgc tcaatgagtt attttattgc
     1441 tgggattagg cgcgtgggca gtaggctgtt tctgcttttc ccacccttac cgtcatgcac
     1501 agtgatgcag ccattatcaa aatcaaaatc tttaacccgc aaacgcaagc attcattaat
     1561 gcgcaaacct gcaccataca gcagcgtaaa aataacttgg ttgcgagtat ccataacctg
     1621 caaaatgcgt tgcacttcat ttgcagagat aacagagggt agccgtctag gcttgcttgc
     1681 agggatataa tcaatatcgc ccaacggctg ttgtaaaaac ctgttgtaca aaaaagctag
     1741 ggcatttaaa gcgattttct gcgtgtttat ggctacatgt ctgctgtttg ctaagctgga
     1801 taaaaacagc ctgacctctt cactgcccat ggtctgagga tgacgttttt tgtgaaacag
     1861 aataaaacgc ttaatccagt gcaggtaagt tttttcagtt ttcagcgcat aacctttttg
     1921 tcgcatatcc gtgcgtatag aatttaaaaa tggactgtta gacataaaac gctccttgtc
     1981 ttgcaactgt ctgcctatac agcctattct agctgggatt taaaaaagtg cctgtttttt
     2041 acgcctagag atgcttgttt accggtagag ttttaattta atgctaaata aattaaaatg
     2101 ttatgagttc tttgggtgag ataatgtgca tcgtgcaagc aggatagacg gcatgcacga
     2161 tttgtaataa cagagtgtct tgtattttta aagaaagtct atttaataca agtgattata
     2221 ttaattaacg gtaagcatca gcgggtgaca aaacgagcat gcttactaat aaaatgttaa
     2281 cctctgagga agaattgtga aactatcact aatggtagct atatcgaaga atggagttat
     2341 cgggaatggc cctgatattc catggagtgc caaaggtgaa cagctcctgt ttaaagctat
     2401 tacctataac caatggctgt tggttggacg caagactttt gaatcaatgg gagcattacc
     2461 caaccgaaag tatgcggtcg taacacgttc aagttttaca tctgacaatg agaacgtatt
     2521 gatctttcca tcaattaaag atgctttaac caacctaaag aaaataacgg atcatgtcat
     2581 tgtttcaggt ggtggggaga tatacaaaag cctgatcgat caagtagata cactacatat
     2641 atctacaata gacatcgagc cggaaggtga tgtttacttt cctgaaatcc ccagcaattt
     2701 taggccagtt tttacccaag acttcgcctc taacataaat tatagttacc aaatctggca
     2761 aaagggttaa caagtggcag caacggattc gcaaacctgt cacgcctttt gtaccaaaag
     2821 ccgcgccagg tttgcgatcc gctgtgccag gcgttaggcg tcatatgaag atttcggtga
     2881 tccctgagca ggtggcggaa acattggatg ctgagaacca tttcattgtt cgtgaagtgt
     2941 tcgatgtgca cctatccgac caaggctttg aactatctac cagaagtgtg agcccctacc
     3001 ggaaggatta catctcggat gatgactctg atgaagactc tgcttgctat ggcgcattca
     3061 tcgaccaaga gcttgtcggg aagattgaac tcaactcaac atggaacgat ctagcctcta
     3121 tcgaacacat tgttgtgtcg cacacgcacc gaggcaaagg agtcgcgcac agtctcatcg
     3181 aatttgcgaa aaagtgggca ctaagcagac agctccttgg catacgatta gagacacaaa
     3241 cgaacaatgt acctgcctgc aatttgtacg caaaatgtgg ctttactctc ggcggcattg
     3301 acctgttcac gtataaaact agacctcaag tctcgaacga aacagcgatg tactggtact
     3361 ggttctcggg agcacaggat gacgcctaac aattcattca agccgacacc gcttcgcggc
     3421 gcggcttaat tcaggagtta gagagctggg gaagactatg cgcgatctgt tgaaggtggt
     3481 tctaagcctc gtacttgcga tggcatcggg gcaggcactt gctgacctgc caattgtttt
     3541 agtggatgaa gctcgtcttc cctatgacta ctccccatcc aactacgaca tttctccaag
     3601 caactacgac aactccataa gcaattacga caatagtcca tcaaattacg acaactctga
     3661 gagcaactac gataatagtt catccaatta cgacaatagt cgcaacggaa atcgtaggct
     3721 tatatatagc gcaaatgggt ctcgcacttt cgccggctac tacgtcattg ccaacaatgg
     3781 gacaacgaac ttcttttcca catctggcaa aaggatgttc tacaccccaa aaggggggcg
     3841 cggcgtctat ggcggcaaag atgggagctt ctgcggggca ttggtcgtca taaatggcca
     3901 attttcgctt gccctgacag ataacggcct gaagatcatg tatctaagca actagcctgc
     3961 tctctaataa aatgttaggc ctcaacatct agtcgcaagc tgaggggaac cactagtgtc
     4021 atacgaacct ccaagagacg gttacacaaa cgggtacatt gttgatgtca tgtatgacaa
     4081 tcgcccaagt aagtatccag ctgtgttcag aacgtacgtc cgaattccag actatgtcga
     4141 actagataac gcggaggagc tggccgacgc aatcattcga gaggttgagt taaaggaggc
     4201 atggctcaca agggtcttgg ggaactcagc cgatcctgac gcttctgtgg gcaagtggga
     4261 ccggcgccta gcgcagtgcg gtggtattga tgagatgtca agtggggtgc aggcaacata
     4321 cttcagtcca aacggtcagc tggtcggctt agtggcatac attgatgccg aatggatgga
     4381 tgaatagctc tgacgttacg attcaggtga gacaaccaga tgggcattgc gtacaagctc
     4441 agttgccacc gatgtggcta cgagtctgat cttctttacc taggccaagg tatggcgatg
     4501 cttcctgagc aaatagtcgg aacgtgcaca tgctgcgaca atctaacgac gatttctata
     4561 aatgaaagct acggagtatg ctcactttgt ggggctaagg atcgtgtgat atttacaagc
     4621 tatcagcatg aggcaaggcc aagaaatccc tgctatctag agagaagttt caaatatcaa
     4681 tgcccaagat gccatgcctt cagtatggag cctccggatc taccggagat gctttgggac
     4741 taatgctccg ctttgcctcg ggtatccgtt cgacct
//
LOCUS       DAJVQG010000493         3871 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.guided.233, whole genome shotgun sequence.
ACCESSION   DAJVQG010000493 DAJVQG010000000
VERSION     DAJVQG010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.guided.233"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            996..1811
                     /gene="sul2"
                     /locus_tag="OQM83_004330"
     CDS             996..1811
                     /gene="sul2"
                     /locus_tag="OQM83_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS1522396.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1872..2675
                     /locus_tag="OQM83_004331"
                     /note="aph(3'')-Ib"
     CDS             1872..2675
                     /locus_tag="OQM83_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCS1522397.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCQFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2675..3511
                     /locus_tag="OQM83_004332"
     CDS             2675..3511
                     /locus_tag="OQM83_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCS1522398.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT          833 a         1088 c         1069 g          881 t
ORIGIN      
        1 ccgcttcctc gctcactgac tcgctacgct cggtcgttcg gctgcggcga gcggtaccgg
       61 cttacggacg gggacggaaa tttcctggaa gataccagga agacacttag caaagaagag
      121 ataaggccac ggcgaagccg tttttccata ggctccgccc ccctgacaag catcgcaaaa
      181 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatcc caggcgtttc
      241 cccctggtag ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
      301 cgctgttatg gccgcggttt ctcattccac gcctgacact cagttccgtg aaggcagttc
      361 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttgtccg
      421 gtaactatca acttgagtcc aacccggaaa gacacgacaa aacgccactg gcagcagcca
      481 ttagtaacag gagtgcacga gaagatagat taagaggtct tgaagtagag gcctgactgc
      541 ggctacactg aaagaacagt tttggtaatg gcggtctcgt gaaccctgtt accgggttaa
      601 gcagttcccc aactgactta accttcgaac aaaccgcctc cccaggcggt tttttcgttt
      661 acagagcaag agattacgat tcattgaaaa ggatctcaag aagatccttt gtagattact
      721 atcaattaat cttccgaaca tgacaccagt cactgttgat tacggttaaa ccgctccgta
      781 acaggtttcc ctcgtattgg tgacttgcag caccaaagaa gcaaaaaaga taaatatatc
      841 tacttattga gagatgatat atttatttcc aaaatgctac agatacatca tcatgaaacg
      901 gatcaccgca aacaggttac tcgcctgggg attccctttc gacccgagca tccgtatgag
      961 actcatgctc gattattatt attatagaag cccccatgaa taaatcgctc atcattttcg
     1021 gcatcgtcaa cataacctcg gacagtttct ccgatggagg ccggtatctg gcgccagacg
     1081 cagccattgc gcaggcgcgt aagctgatgg ccgagggggc agatgtgatc gacctcggtc
     1141 cggcatccag caatcccgac gccgcgcctg tttcgtccga cacagaaatc gcgcgtatcg
     1201 cgccggtgct ggacgcgctc aaggcagatg gcattcccgt ctcgctcgac agttatcaac
     1261 ccgcgacgca agcctatgcc ttgtcgcgtg gtgtggccta tctcaatgat attcgcggtt
     1321 ttccagacgc tgcgttctat ccgcaattgg cgaaatcatc tgccaaactc gtcgttatgc
     1381 attcggtgca agacgggcag gcagatcggc gcgaggcacc cgctggcgac atcatggatc
     1441 acattgcggc gttctttgac gcgcgcatcg cggcgctgac gggtgccggt atcaaacgca
     1501 accgccttgt ccttgatccc ggcatggggt tttttctggg ggctgctccc gaaacctcgc
     1561 tctcggtgct ggcgcggttc gatgaattgc ggctgcgctt cgatttgccg gtgcttctgt
     1621 ctgtttcgcg caaatccttt ctgcgcgcgc tcacaggccg tggtccgggg gatgtcgggg
     1681 ccgcgacact cgctgcagag cttgccgccg ccgcaggtgg agctgacttc atccgcacac
     1741 acgagccgcg ccccttgcgc gacgggctgg cggtattggc ggcgctgaaa gaaaccgcaa
     1801 gaattcgtta actgcacatt cgggatattt ctctatattc gcgcttcatc agaaaactga
     1861 aggaacctcc attgaatcga actaatattt tttttggtga atcgcattct gactggttgc
     1921 ctgtcagagg cggagaatct ggtgattttg tttttcgacg tggtgacggg catgccttcg
     1981 cgaaaatcgc acctgcttcc cgccgcggtg agctcgctgg agagcgtgac cgcctcattt
     2041 ggctcaaagg tcgaggtgtg gcttgccccg aggtcatcaa ctggcaggag gaacaggagg
     2101 gtgcatgctt ggtgataacg gcaattccgg gagtaccggc ggctgatctg tctggagcgg
     2161 atttgctcaa agcgtggccg tcaatggggc agcaacttgg cgctgttcac agcctatcgg
     2221 ttgatcaatg tcagtttgag cgcaggctgt cgcgaatgtt cggacgcgcc gttgatgtgg
     2281 tgtcccgcaa tgccgtcaat cccgacttct taccggacga ggacaagagt acgccgcagc
     2341 tcgatctttt ggctcgtgtc gaacgagagc taccggtgcg gctcgaccaa gagcgcaccg
     2401 atatggttgt ttgccatggt gatccctgca tgccgaactt catggtggac cctaaaactc
     2461 ttcaatgcac gggtctgatc gaccttgggc ggctcggaac agcagatcgc tatgccgatt
     2521 tggcactcat gattgctaac gccgaagaga actgggcagc gccagatgaa gcagagcgcg
     2581 ccttcgctgt cctattcaat gtattgggga tcgaagcccc cgaccgcgaa cgccttgcct
     2641 tctatctgcg attggaccct ctgacttggg gttgatgttc atgccgcctg tttttcctgc
     2701 tcattggcac gtttcgcaac ctgttctcat tgcggacacc ttttccagcc tcgtttggaa
     2761 agtttcattg ccagacggga ctcctgcaat cgtcaaggga ttgaaaccta tagaagacat
     2821 tgctgatgaa ctgcgcgggg ccgactatct ggtatggcgc aatgggaggg gagcagtccg
     2881 gttgctcggt cgtgagaaca atctgatgtt gctcgaatat gccggggagc gaatgctctc
     2941 tcacatcgtt gccgagcacg gcgactacca ggcgaccgaa attgcagcgg aactaatggc
     3001 gaagctgtat gccgcatctg aggaacccct gccttctgcc cttctcccga tccgggatcg
     3061 ctttgcagct ttgtttcagc gggcgcgcga tgatcaaaac gcaggttgtc aaactgacta
     3121 cgtccacgcg gcgattatag ccgatcaaat gatgagcaat gcctcggaac tgcgtgggct
     3181 acatggcgat ctgcatcatg aaaacatcat gttctccagt cgcggctggc tggtgataga
     3241 tcccgtcggt ctggtcggtg aagtgggctt tggcgccgcc aatatgttct acgatccggc
     3301 tgacagagac gacctttgtc tcgatcctag acgcattgca cagatggcgg acgcattctc
     3361 tcgtgcgctg gacgtcgatc cgcgtcgcct gctcgaccag gcgtacgctt atgggtgcct
     3421 ttccgcagct tggaacgcgg atggagaaga ggagcaacgc gatctagcta tcgcggccgc
     3481 gatcaagcag gtgcgacaga cgtcatacta gatatcaagc gacttctcct atcccctggg
     3541 aacacatcaa tctcaccgga gaatatcgct ggccaaagcc ttagcgtagg attccgcccc
     3601 ttcccgcaaa cgacccctgg aagccccgta gcgacgcgga gaggggcgag acaagccaag
     3661 ggcgcaggct cgatgcgcag cacgacatag ccggttctcg caaggacgag aatttccctg
     3721 cggtgcccct caagtgtcaa tgaaagtttc caacgcgagc cattcgcgag agccttgagt
     3781 ccacgctaga tctatctcat ctgcgcaagg cagaacgtga agacggccgc cctggacctc
     3841 gcccgcgagc gccaggcgca cgaggccggc g
//
LOCUS       DAJVQG010000494         3870 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.guided.234, whole genome shotgun sequence.
ACCESSION   DAJVQG010000494 DAJVQG010000000
VERSION     DAJVQG010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.guided.234"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            996..1811
                     /gene="sul2"
                     /locus_tag="OQM83_004333"
     CDS             996..1811
                     /gene="sul2"
                     /locus_tag="OQM83_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS1522399.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1916..2674
                     /locus_tag="OQM83_004334"
     CDS             1916..2674
                     /locus_tag="OQM83_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3'') family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCS1522400.1"
                     /translation="MPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGERDRLIWLK
                     GRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLGAVHSLSV
                     DQCQFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELPVRLDQER
                     TDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEENWAAPDE
                     AERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2674..3510
                     /locus_tag="OQM83_004335"
     CDS             2674..3510
                     /locus_tag="OQM83_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCS1522401.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT          833 a         1088 c         1069 g          880 t
ORIGIN      
        1 ccgcttcctc gctcactgac tcgctacgct cggtcgttcg gctgcggcga gcggtaccgg
       61 cttacggacg gggacggaaa tttcctggaa gataccagga agacacttag caaagaagag
      121 ataaggccac ggcgaagccg tttttccata ggctccgccc ccctgacaag catcgcaaaa
      181 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatcc caggcgtttc
      241 cccctggtag ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
      301 cgctgttatg gccgcggttt ctcattccac gcctgacact cagttccgtg aaggcagttc
      361 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttgtccg
      421 gtaactatca acttgagtcc aacccggaaa gacacgacaa aacgccactg gcagcagcca
      481 ttagtaacag gagtgcacga gaagatagat taagaggtct tgaagtagag gcctgactgc
      541 ggctacactg aaagaacagt tttggtaatg gcggtctcgt gaaccctgtt accgggttaa
      601 gcagttcccc aactgactta accttcgaac aaaccgcctc cccaggcggt tttttcgttt
      661 acagagcaag agattacgat tcattgaaaa ggatctcaag aagatccttt gtagattact
      721 atcaattaat cttccgaaca tgacaccagt cactgttgat tacggttaaa ccgctccgta
      781 acaggtttcc ctcgtattgg tgacttgcag caccaaagaa gcaaaaaaga taaatatatc
      841 tacttattga gagatgatat atttatttcc aaaatgctac agatacatca tcatgaaacg
      901 gatcaccgca aacaggttac tcgcctgggg attccctttc gacccgagca tccgtatgag
      961 actcatgctc gattattatt attatagaag cccccatgaa taaatcgctc atcattttcg
     1021 gcatcgtcaa cataacctcg gacagtttct ccgatggagg ccggtatctg gcgccagacg
     1081 cagccattgc gcaggcgcgt aagctgatgg ccgagggggc agatgtgatc gacctcggtc
     1141 cggcatccag caatcccgac gccgcgcctg tttcgtccga cacagaaatc gcgcgtatcg
     1201 cgccggtgct ggacgcgctc aaggcagatg gcattcccgt ctcgctcgac agttatcaac
     1261 ccgcgacgca agcctatgcc ttgtcgcgtg gtgtggccta tctcaatgat attcgcggtt
     1321 ttccagacgc tgcgttctat ccgcaattgg cgaaatcatc tgccaaactc gtcgttatgc
     1381 attcggtgca agacgggcag gcagatcggc gcgaggcacc cgctggcgac atcatggatc
     1441 acattgcggc gttctttgac gcgcgcatcg cggcgctgac gggtgccggt atcaaacgca
     1501 accgccttgt ccttgatccc ggcatggggt tttttctggg ggctgctccc gaaacctcgc
     1561 tctcggtgct ggcgcggttc gatgaattgc ggctgcgctt cgatttgccg gtgcttctgt
     1621 ctgtttcgcg caaatccttt ctgcgcgcgc tcacaggccg tggtccgggg gatgtcgggg
     1681 ccgcgacact cgctgcagag cttgccgccg ccgcaggtgg agctgacttc atccgcacac
     1741 acgagccgcg ccccttgcgc gacgggctgg cggtattggc ggcgctgaaa gaaaccgcaa
     1801 gaattcgtta actgcacatt cgggatattt ctctatattc gcgcttcatc agaaaactga
     1861 aggaacctcc attgaatcga actaatattt ttttggtgaa tcgcattctg actggttgcc
     1921 tgtcagaggc ggagaatctg gtgattttgt ttttcgacgt ggtgacgggc atgccttcgc
     1981 gaaaatcgca cctgcttccc gccgcggtga gctcgctgga gagcgtgacc gcctcatttg
     2041 gctcaaaggt cgaggtgtgg cttgccccga ggtcatcaac tggcaggagg aacaggaggg
     2101 tgcatgcttg gtgataacgg caattccggg agtaccggcg gctgatctgt ctggagcgga
     2161 tttgctcaaa gcgtggccgt caatggggca gcaacttggc gctgttcaca gcctatcggt
     2221 tgatcaatgt cagtttgagc gcaggctgtc gcgaatgttc ggacgcgccg ttgatgtggt
     2281 gtcccgcaat gccgtcaatc ccgacttctt accggacgag gacaagagta cgccgcagct
     2341 cgatcttttg gctcgtgtcg aacgagagct accggtgcgg ctcgaccaag agcgcaccga
     2401 tatggttgtt tgccatggtg atccctgcat gccgaacttc atggtggacc ctaaaactct
     2461 tcaatgcacg ggtctgatcg accttgggcg gctcggaaca gcagatcgct atgccgattt
     2521 ggcactcatg attgctaacg ccgaagagaa ctgggcagcg ccagatgaag cagagcgcgc
     2581 cttcgctgtc ctattcaatg tattggggat cgaagccccc gaccgcgaac gccttgcctt
     2641 ctatctgcga ttggaccctc tgacttgggg ttgatgttca tgccgcctgt ttttcctgct
     2701 cattggcacg tttcgcaacc tgttctcatt gcggacacct tttccagcct cgtttggaaa
     2761 gtttcattgc cagacgggac tcctgcaatc gtcaagggat tgaaacctat agaagacatt
     2821 gctgatgaac tgcgcggggc cgactatctg gtatggcgca atgggagggg agcagtccgg
     2881 ttgctcggtc gtgagaacaa tctgatgttg ctcgaatatg ccggggagcg aatgctctct
     2941 cacatcgttg ccgagcacgg cgactaccag gcgaccgaaa ttgcagcgga actaatggcg
     3001 aagctgtatg ccgcatctga ggaacccctg ccttctgccc ttctcccgat ccgggatcgc
     3061 tttgcagctt tgtttcagcg ggcgcgcgat gatcaaaacg caggttgtca aactgactac
     3121 gtccacgcgg cgattatagc cgatcaaatg atgagcaatg cctcggaact gcgtgggcta
     3181 catggcgatc tgcatcatga aaacatcatg ttctccagtc gcggctggct ggtgatagat
     3241 cccgtcggtc tggtcggtga agtgggcttt ggcgccgcca atatgttcta cgatccggct
     3301 gacagagacg acctttgtct cgatcctaga cgcattgcac agatggcgga cgcattctct
     3361 cgtgcgctgg acgtcgatcc gcgtcgcctg ctcgaccagg cgtacgctta tgggtgcctt
     3421 tccgcagctt ggaacgcgga tggagaagag gagcaacgcg atctagctat cgcggccgcg
     3481 atcaagcagg tgcgacagac gtcatactag atatcaagcg acttctccta tcccctggga
     3541 acacatcaat ctcaccggag aatatcgctg gccaaagcct tagcgtagga ttccgcccct
     3601 tcccgcaaac gacccctgga agccccgtag cgacgcggag aggggcgaga caagccaagg
     3661 gcgcaggctc gatgcgcagc acgacatagc cggttctcgc aaggacgaga atttccctgc
     3721 ggtgcccctc aagtgtcaat gaaagtttcc aacgcgagcc attcgcgaga gccttgagtc
     3781 cacgctagat ctatctcatc tgcgcaaggc agaacgtgaa gacggccgcc ctggacctcg
     3841 cccgcgagcg ccaggcgcac gaggccggcg
//
LOCUS       DAJVQG010000495         3817 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 602729
            SAMEA8900614-rid15854523.guided.236, whole genome shotgun sequence.
ACCESSION   DAJVQG010000495 DAJVQG010000000
VERSION     DAJVQG010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900614
            Sequence Read Archive: ERR6005554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478468.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478468.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,341
            CDSs (total)                      :: 4,269
            Genes (coding)                    :: 4,030
            CDSs (with protein)               :: 4,030
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 116 of 239
            Pseudo Genes (incomplete)         :: 59 of 239
            Pseudo Genes (internal stop)      :: 87 of 239
            Pseudo Genes (multiple problems)  :: 23 of 239
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900614-rid15854523.guided.236"
                     /serovar="S. sonnei"
                     /isolate="602729"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-06-18"
     gene            <1..171
                     /locus_tag="OQM83_004336"
     CDS             <1..171
                     /locus_tag="OQM83_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HCS1522402.1"
                     /translation="AHERTPQQERQKAAREAERGREAWTLGQGMKKPVAGCYGRLTRW
                     KGGGDVVYMALL"
     gene            complement(203..853)
                     /gene="tetR(A)"
                     /locus_tag="OQM83_004337"
     CDS             complement(203..853)
                     /gene="tetR(A)"
                     /locus_tag="OQM83_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCS1522403.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            959..2158
                     /gene="tet(A)"
                     /locus_tag="OQM83_004338"
     CDS             959..2158
                     /gene="tet(A)"
                     /locus_tag="OQM83_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCS1522404.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            complement(2125..2313)
                     /locus_tag="OQM83_004339"
                     /pseudo
     CDS             complement(2125..2313)
                     /locus_tag="OQM83_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(2357..2746)
                     /locus_tag="OQM83_004340"
     CDS             complement(2357..2746)
                     /locus_tag="OQM83_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1522405.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            2964..3404
                     /locus_tag="OQM83_004341"
     CDS             2964..3404
                     /locus_tag="OQM83_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCS1522406.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT          806 a         1090 c         1080 g          841 t
ORIGIN      
        1 gcccacgagc ggacgccgca gcaggagcgc cagaaggccg ccagagaggc cgagcgcggc
       61 cgtgaggctt ggacgctagg gcagggcatg aaaaagcccg tagcgggctg ctacgggcgt
      121 ctgacgcggt ggaaaggggg aggggatgtt gtctacatgg ctctgctgta gtgagtgggt
      181 tgcgctccgg cagcggtcct gatcaatcgt caccctttct cggtccttca acgttcctga
      241 caacgagcct ccttttcgcc aatccatcga caatcaccgc gagtccctgc tcgaacgctg
      301 cgtccggacc ggcttcgtcg aaggcgtcta tcgcggcccg caacagcggc gagagcggag
      361 cctgttcaac ggtgccgccg cgctcgccgg catcgctgtc gccggcctgc tcctcaagca
      421 cggccccaac agtgaagtag ctgattgtca tcagcgcatt gacggcgtcc ccggccgaaa
      481 aacccgcctc gcagaggaag cgaagctgcg cgtcggccgt ttccatctgc ggtgcgcccg
      541 gtcgcgtgcc ggcatggatg cgcgcgccat cgcggtaggc gagcagcgcc tgcctgaagc
      601 tgcgggcatt cccgatcaga aatgagcgcc agtcgtcgtc ggctctcggc accgaatgcg
      661 tatgattctc cgccagcatg gcttcggcca gtgcgtcgag cagcgcccgc ttgttcctga
      721 agtgccagta aagcgccggc tgctgaaccc ccaaccgttc cgccagtttg cgtgtcgtca
      781 gaccgtctac gccgacctcg ttcaacaggt ccagggcggc acggatcact gtattcggct
      841 gcaactttgt catgcttgac actttatcac tgataaacat aatatgtcca ccaacttatc
      901 agtgataaag aatccgcgcg ttcaatcgga ccagcggagg ctggtccgga ggccagacgt
      961 gaaacccaac agacccctga tcgtaattct gagcactgtc gcgctcgacg ctgtcggcat
     1021 cggcctgatt atgccggtgc tgccgggcct cctgcgcgat ctggttcact cgaacgacgt
     1081 caccgcccac tatggcattc tgctggcgct gtatgcgttg atgcaatttg cctgcgcacc
     1141 tgtgctgggc gcgctgtcgg atcgtttcgg gcggcggccg gtcttgctcg tctcgctggc
     1201 cggcgctgct gtcgactacg ccatcatggc gacggcgcct ttcctttggg ttctctatat
     1261 cgggcggatc gtggccggca tcaccggggc gactggggcg gtagccggcg cttatattgc
     1321 cgatatcact gatggcgatg agcgcgcgcg gcacttcggc ttcatgagcg cctgtttcgg
     1381 gttcgggatg gtcgcgggac ctgtgctcgg tgggctgatg ggcggtttct ccccccacgc
     1441 tccgttcttc gccgcggcag ccttgaacgg cctcaatttc ctgacgggct gtttcctttt
     1501 gccggagtcg cacaaaggcg aacgccggcc gttacgccgg gaggctctca acccgctcgc
     1561 ttcgttccgg tgggcccggg gcatgaccgt cgtcgccgcc ctgatggcgg tcttcttcat
     1621 catgcaactt gtcggacagg tgccggccgc gctttgggtc attttcggcg aggatcgctt
     1681 tcactgggac gcgaccacga tcggcatttc gcttgccgca tttggcattc tgcattcact
     1741 cgcccaggca atgatcaccg gccctgtagc cgcccggctc ggcgaaaggc gggcactcat
     1801 gctcggaatg attgccgacg gcacaggcta catcctgctt gccttcgcga cacggggatg
     1861 gatggcgttc ccgatcatgg tcctgcttgc ttcgggtggc atcggaatgc cggcgctgca
     1921 agcaatgttg tccaggcagg tggatgagga acgtcagggg cagctgcaag gctcactggc
     1981 ggcgctcacc agcctgacct cgatcgtcgg acccctcctc ttcacggcga tctatgcggc
     2041 ttctataaca acgtggaacg ggtgggcatg gattgcaggc gctgccctct acttgctctg
     2101 cctgccggcg ctgcgtcgcg ggctttggag aaattcttca aattcccgtt gcacatagcc
     2161 cggcaattcc tttccctgct ctgccataag cgcagcgaat gccgggtaat actcgtcaac
     2221 gatctgatag agaagggttt gctcgggtcg gtggctctgg taacgaccag tatcccgatc
     2281 ccggctggcc gtcctggccg ccacatgagg catgttccgc gtccttgcaa tactgtgttt
     2341 acatacagtc tatcgcttag cggaaagttc ttttaccctc agccgaaatg cctgccgttg
     2401 ctagacattg ccagccagtg cccgtcactc ccactcttac attgttcgca gtatctaggt
     2461 acatgttatt gcgtgcacct tcatgatgta taatgttatt aatgacagca gaaagatcga
     2521 cagaattaat attcttcgta cctgggacag ttatcgggtg tcggccagtg atgagaccaa
     2581 aggatgcagt ataagatgaa ccactactat tatcatggcc accattacca ccaccgttaa
     2641 aactacctcc cgaacttcca gagccgctat gaccatcatt gtaggggtca tttggtccca
     2701 tgtgaccaaa accaccgaca gtaccgctat ttaactctgt actcataaaa ttatcctctt
     2761 tgacttttaa aaacagcaaa aactgtattt ataacaatgt gtttatatac agtattcgaa
     2821 atactacgcc cgtcaaaacc acaccgtcaa caggtttttt gtgtattttt cacgcctcac
     2881 gggcagcctc agaccttttg tgttaaaaca gtaatatgtt ctgctgttac aatacggtac
     2941 acacaggaga cactgtcact tttatggcat ggcacacagt tcaagagaca ctgaaattaa
     3001 caggaaagtc gcgctcgcaa ttatatcgtg acatggctaa aggccttgtc agctatcgca
     3061 caggaaaaaa tgatcgccgg gagttcgaga ccagtgaact gattcgggcc tatggcgagc
     3121 tgcgcacttc tgagacacca aaaaggcaca aggagggaca ctccaaatct gtcgttagcc
     3181 aggcggaaaa tgctcagcta gacgtcattc agcagaaact ggacgctctg cagcagactg
     3241 ttaccctaat gcttgaagac aagatggcgc gggaagcaga aaaccgcctg cgaaatgagg
     3301 agagggaaca actacaggcc gaaattagcc gtctggctga cgcgctggca caggaaaaaa
     3361 agcggggatt ctggtcgcgg ctgtttcgct ccggttcaga gtaacagtgc aggtgactct
     3421 gatttaccat aacatctgtt atcagcaccg cctgagaggc cctcagggcg tttctgacgg
     3481 gaaaccggta aaacaggcaa caaaccaccc gaaaacccgc cagaaacgcg caggctctgt
     3541 ttttacggca tgcatgagta tgcgcacaag tgcatagcgc ggaaattcac accattcagc
     3601 cggggtaagt cctgtttccg gcgttcttcc catccgaaaa cggtgaacgg ggacgcaatt
     3661 tattgcgtgg ccgtgaagcg ttgccggcgc agccggaaaa cccgctgtca gcacgttcag
     3721 gaagggggtg atgtacaaat ggcacagagg atgaaagtga cgtatgagct gaaggaatga
     3781 tgtaaaagtg atgtataagt gatgtaaaag tgatgta
//
